LOCUS       DAJTYG010000001       139756 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTYG010000001 DAJTYG010000000
VERSION     DAJTYG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 139756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 139756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..139756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.001"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO568_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO568_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6639524.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO568_000002"
     CDS             complement(1672..2730)
                     /locus_tag="OO568_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6639525.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO568_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO568_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO568_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO568_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6639526.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO568_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO568_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6639527.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO568_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO568_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6639528.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO568_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO568_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6639529.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO568_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO568_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6639530.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO568_000009"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO568_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6639531.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO568_000010"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO568_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6639532.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO568_000011"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO568_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6639533.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIHYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRNILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO568_000012"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO568_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6639534.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO568_000013"
     CDS             complement(17699..18322)
                     /locus_tag="OO568_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6639535.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OO568_000014"
     CDS             18619..18894
                     /locus_tag="OO568_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639536.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO568_000015"
     CDS             complement(18971..19213)
                     /locus_tag="OO568_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6639537.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO568_000016"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO568_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6639538.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OO568_000017"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OO568_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6639539.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO568_000018"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO568_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6639540.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGETVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22592..24289
                     /gene="ypdA"
                     /locus_tag="OO568_000019"
     CDS             22592..24289
                     /gene="ypdA"
                     /locus_tag="OO568_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR6639541.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTPRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24304..25038
                     /gene="ypdB"
                     /locus_tag="OO568_000020"
     CDS             24304..25038
                     /gene="ypdB"
                     /locus_tag="OO568_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6639542.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            25051..25908
                     /locus_tag="OO568_000021"
     CDS             25051..25908
                     /locus_tag="OO568_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6639543.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25911..28412)
                     /gene="ptsP"
                     /locus_tag="OO568_000022"
     CDS             complement(25911..28412)
                     /gene="ptsP"
                     /locus_tag="OO568_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6639544.1"
                     /translation="MLTIQFLCPLPMPNGLHARPARELKEQCSQWQSEITFINHRQNA
                     KADAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAEL
                     TAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHS
                     LATLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNM
                     EQVCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTP
                     SQFLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQ
                     CGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEA
                     PGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGD
                     KSISYLNVPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLD
                     QILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSND
                     MTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLP
                     LLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPE
                     EDVRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTG
                     VGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVF
                     SQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28437..29474)
                     /gene="ypdE"
                     /locus_tag="OO568_000023"
     CDS             complement(28437..29474)
                     /gene="ypdE"
                     /locus_tag="OO568_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6639545.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKILGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29474..30557)
                     /gene="ypdF"
                     /locus_tag="OO568_000024"
                     /pseudo
     CDS             complement(29474..30557)
                     /gene="ypdF"
                     /locus_tag="OO568_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30572..31819)
                     /locus_tag="OO568_000025"
     CDS             complement(30572..31819)
                     /locus_tag="OO568_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6639546.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGENGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31841..32167)
                     /locus_tag="OO568_000026"
     CDS             complement(31841..32167)
                     /locus_tag="OO568_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6639547.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32386..33351)
                     /gene="glk"
                     /locus_tag="OO568_000027"
     CDS             complement(32386..33351)
                     /gene="glk"
                     /locus_tag="OO568_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6639548.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33555..34811
                     /locus_tag="OO568_000028"
     CDS             33555..34811
                     /locus_tag="OO568_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6639549.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAIMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34926..35252
                     /locus_tag="OO568_000029"
     CDS             34926..35252
                     /locus_tag="OO568_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6639550.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35299..36537)
                     /locus_tag="OO568_000030"
     CDS             complement(35299..36537)
                     /locus_tag="OO568_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6639551.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36873..38075
                     /gene="nupC"
                     /locus_tag="OO568_000031"
     CDS             36873..38075
                     /gene="nupC"
                     /locus_tag="OO568_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6639552.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38125..40314)
                     /locus_tag="OO568_000032"
                     /pseudo
     CDS             complement(38125..40314)
                     /locus_tag="OO568_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40522..40597)
                     /locus_tag="OO568_000033"
     tRNA            complement(40522..40597)
                     /locus_tag="OO568_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40562..40564),aa:Ala,seq:ggc)
     gene            complement(40637..40712)
                     /locus_tag="OO568_000034"
     tRNA            complement(40637..40712)
                     /locus_tag="OO568_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40677..40679),aa:Ala,seq:ggc)
     gene            40933..41292
                     /locus_tag="OO568_000035"
     CDS             40933..41292
                     /locus_tag="OO568_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6639553.1"
                     /translation="MSKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41294..41686
                     /locus_tag="OO568_000036"
     CDS             41294..41686
                     /locus_tag="OO568_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6639554.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41738..43153)
                     /gene="gltX"
                     /locus_tag="OO568_000037"
     CDS             complement(41738..43153)
                     /gene="gltX"
                     /locus_tag="OO568_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6639555.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43412..43487
                     /locus_tag="OO568_000038"
     tRNA            43412..43487
                     /locus_tag="OO568_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43445..43447,aa:Val,seq:tac)
     gene            43532..43607
                     /locus_tag="OO568_000039"
     tRNA            43532..43607
                     /locus_tag="OO568_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43565..43567,aa:Val,seq:tac)
     gene            43612..43687
                     /locus_tag="OO568_000040"
     tRNA            43612..43687
                     /locus_tag="OO568_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43645..43647,aa:Lys,seq:ttt)
     gene            43718..43843
                     /locus_tag="OO568_000041"
     ncRNA           43718..43843
                     /ncRNA_class="other"
                     /locus_tag="OO568_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43963..44304
                     /locus_tag="OO568_000042"
     CDS             43963..44304
                     /locus_tag="OO568_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6639556.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44295..45221)
                     /locus_tag="OO568_000043"
     CDS             complement(44295..45221)
                     /locus_tag="OO568_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6639557.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45311..46309
                     /locus_tag="OO568_000044"
     CDS             45311..46309
                     /locus_tag="OO568_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6639558.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46306..46524)
                     /locus_tag="OO568_000045"
     CDS             complement(46306..46524)
                     /locus_tag="OO568_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6639559.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46526..48541)
                     /gene="ligA"
                     /locus_tag="OO568_000046"
     CDS             complement(46526..48541)
                     /gene="ligA"
                     /locus_tag="OO568_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6639560.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGSTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48612..49499)
                     /gene="zipA"
                     /locus_tag="OO568_000047"
     CDS             complement(48612..49499)
                     /gene="zipA"
                     /locus_tag="OO568_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6639561.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEA
                     VIIMNVAAHHGSELNGELLLNSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKP
                     GTFDPEMKDFTTPGVTIFMQVPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMT
                     PQKLREYQDIIREVKDANA"
     gene            49729..50490
                     /gene="cysZ"
                     /locus_tag="OO568_000048"
     CDS             49729..50490
                     /gene="cysZ"
                     /locus_tag="OO568_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6639562.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50675..51646
                     /gene="cysK"
                     /locus_tag="OO568_000049"
     CDS             50675..51646
                     /gene="cysK"
                     /locus_tag="OO568_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6639563.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            52030..52287
                     /gene="ptsH"
                     /locus_tag="OO568_000050"
     CDS             52030..52287
                     /gene="ptsH"
                     /locus_tag="OO568_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6639564.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52332..54059
                     /gene="ptsI"
                     /locus_tag="OO568_000051"
     CDS             52332..54059
                     /gene="ptsI"
                     /locus_tag="OO568_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6639565.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVAQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            54100..54609
                     /gene="crr"
                     /locus_tag="OO568_000052"
     CDS             54100..54609
                     /gene="crr"
                     /locus_tag="OO568_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6639566.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54651..55502)
                     /gene="pdxK"
                     /locus_tag="OO568_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54651..55502)
                     /gene="pdxK"
                     /locus_tag="OO568_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6639567.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55607..55981
                     /locus_tag="OO568_000054"
     CDS             55607..55981
                     /locus_tag="OO568_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6639568.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            56014..56737
                     /locus_tag="OO568_000055"
                     /pseudo
     CDS             56014..56737
                     /locus_tag="OO568_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56941..57852)
                     /gene="cysM"
                     /locus_tag="OO568_000056"
     CDS             complement(56941..57852)
                     /gene="cysM"
                     /locus_tag="OO568_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6639569.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57986..59083)
                     /gene="cysA"
                     /locus_tag="OO568_000057"
     CDS             complement(57986..59083)
                     /gene="cysA"
                     /locus_tag="OO568_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6639570.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(59073..59948)
                     /gene="cysW"
                     /locus_tag="OO568_000058"
     CDS             complement(59073..59948)
                     /gene="cysW"
                     /locus_tag="OO568_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6639571.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59948..60781)
                     /gene="cysT"
                     /locus_tag="OO568_000059"
     CDS             complement(59948..60781)
                     /gene="cysT"
                     /locus_tag="OO568_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6639572.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60781..61797)
                     /gene="cysP"
                     /locus_tag="OO568_000060"
     CDS             complement(60781..61797)
                     /gene="cysP"
                     /locus_tag="OO568_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6639573.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDEAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62101..62892)
                     /gene="ucpA"
                     /locus_tag="OO568_000061"
     CDS             complement(62101..62892)
                     /gene="ucpA"
                     /locus_tag="OO568_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6639574.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(63079..63879)
                     /gene="murR"
                     /locus_tag="OO568_000062"
     CDS             complement(63079..63879)
                     /gene="murR"
                     /locus_tag="OO568_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6639575.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            64043..64939
                     /gene="murQ"
                     /locus_tag="OO568_000063"
     CDS             64043..64939
                     /gene="murQ"
                     /locus_tag="OO568_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6639576.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64943..66367
                     /gene="murP"
                     /locus_tag="OO568_000064"
     CDS             64943..66367
                     /gene="murP"
                     /locus_tag="OO568_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR6639577.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAARACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66547..67446)
                     /gene="yfeX"
                     /locus_tag="OO568_000065"
     CDS             complement(66547..67446)
                     /gene="yfeX"
                     /locus_tag="OO568_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6639578.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMVL"
     gene            complement(67542..68117)
                     /locus_tag="OO568_000066"
     CDS             complement(67542..68117)
                     /locus_tag="OO568_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6639579.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68178..68627)
                     /locus_tag="OO568_000067"
     CDS             complement(68178..68627)
                     /locus_tag="OO568_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6639580.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68614..69039)
                     /locus_tag="OO568_000068"
     CDS             complement(68614..69039)
                     /locus_tag="OO568_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6639581.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLSPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69253..70122
                     /gene="amiA"
                     /locus_tag="OO568_000069"
     CDS             69253..70122
                     /gene="amiA"
                     /locus_tag="OO568_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6639582.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70126..71025
                     /gene="hemF"
                     /locus_tag="OO568_000070"
     CDS             70126..71025
                     /gene="hemF"
                     /locus_tag="OO568_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6639583.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(71031..72084)
                     /gene="eutR"
                     /locus_tag="OO568_000071"
                     /pseudo
     CDS             complement(71031..72084)
                     /gene="eutR"
                     /locus_tag="OO568_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72169..72630)
                     /gene="eutK"
                     /locus_tag="OO568_000072"
     CDS             complement(72169..72630)
                     /gene="eutK"
                     /locus_tag="OO568_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6639584.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGT"
     gene            complement(72643..73302)
                     /gene="eutL"
                     /locus_tag="OO568_000073"
     CDS             complement(72643..73302)
                     /gene="eutL"
                     /locus_tag="OO568_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6639585.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(73312..74202)
                     /gene="eutC"
                     /locus_tag="OO568_000074"
     CDS             complement(73312..74202)
                     /gene="eutC"
                     /locus_tag="OO568_000074"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCR6639586.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVAANP
                     DVQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEIL
                     GAKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAV
                     IVDLAKRMLEQKASGINMTR"
     gene            complement(74223..75584)
                     /gene="eutB"
                     /locus_tag="OO568_000075"
     CDS             complement(74223..75584)
                     /gene="eutB"
                     /locus_tag="OO568_000075"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HCR6639587.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAIAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDGCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            75803..77023
                     /locus_tag="OO568_000076"
     CDS             75803..77023
                     /locus_tag="OO568_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6639588.1"
                     /translation="MDAAQPKDKQYKLTASLTPGLFLLVHPNGSKYWRFRYWIDKKER
                     LQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNMAAGFKTVAEDW
                     LATRVGGWSESYTKQVKSALEKDVYPVLGKRSIVDITARDVLALLQKKERTAPEQARK
                     LRQRIGEIFKFAVITELVTRNPVADLDTALKARRPGHNAWIPISEIPAFYKALERAGS
                     VQIQAAIRLLILTALRTAELRLCRWEWINLEDATITLPAEVMKARRPHVVPLSRQAVE
                     LLQDQFTRSGYSVFVFPGRFMDKPLSASAILKALERIGYKSIATGHGWRTTFSTALNE
                     SGRYNPDWIEIQLAHVPKGIRGVYNQAAYLKMRRQMLQDYAHCRSARSNPHDKAVRIV
                     TIMLKDSLNHSLTN"
     gene            77488..78240
                     /locus_tag="OO568_000077"
     CDS             77488..78240
                     /locus_tag="OO568_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639589.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIYTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCRE"
     gene            79000..79395
                     /locus_tag="OO568_000078"
     CDS             79000..79395
                     /locus_tag="OO568_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6639590.1"
                     /translation="MNNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLDKQDNRIKNIRLMPKEK
                     YRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            79413..79676
                     /locus_tag="OO568_000079"
     CDS             79413..79676
                     /locus_tag="OO568_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639591.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            79699..80241
                     /locus_tag="OO568_000080"
     CDS             79699..80241
                     /locus_tag="OO568_000080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639592.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            80706..80912
                     /locus_tag="OO568_000081"
     CDS             80706..80912
                     /locus_tag="OO568_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639593.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            81224..81709
                     /locus_tag="OO568_000082"
     CDS             81224..81709
                     /locus_tag="OO568_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6639594.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            81690..81968
                     /locus_tag="OO568_000083"
     CDS             81690..81968
                     /locus_tag="OO568_000083"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639595.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            81961..83421
                     /locus_tag="OO568_000084"
     CDS             81961..83421
                     /locus_tag="OO568_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6639596.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            83455..84264
                     /gene="murI"
                     /locus_tag="OO568_000085"
     CDS             83455..84264
                     /gene="murI"
                     /locus_tag="OO568_000085"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6639597.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEKYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(84383..85077)
                     /locus_tag="OO568_000086"
     CDS             complement(join(84383..84831,84831..85077))
                     /locus_tag="OO568_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6639598.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(85601..86974)
                     /gene="eutA"
                     /locus_tag="OO568_000087"
     CDS             complement(85601..86974)
                     /gene="eutA"
                     /locus_tag="OO568_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6639599.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVSDLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVDVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVCATVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(86949..87428)
                     /gene="eutP"
                     /locus_tag="OO568_000088"
     CDS             complement(86949..87428)
                     /gene="eutP"
                     /locus_tag="OO568_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6639600.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIVVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(87441..87776)
                     /gene="eutS"
                     /locus_tag="OO568_000089"
     CDS             complement(87441..87776)
                     /gene="eutS"
                     /locus_tag="OO568_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6639601.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(88069..90348)
                     /gene="maeB"
                     /locus_tag="OO568_000090"
     CDS             complement(88069..90348)
                     /gene="maeB"
                     /locus_tag="OO568_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6639602.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFVADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            90637..91587
                     /gene="tal"
                     /locus_tag="OO568_000091"
     CDS             90637..91587
                     /gene="tal"
                     /locus_tag="OO568_000091"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6639603.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPLDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            91607..93610
                     /gene="tkt"
                     /locus_tag="OO568_000092"
     CDS             91607..93610
                     /gene="tkt"
                     /locus_tag="OO568_000092"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6639604.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATCLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(93713..94780)
                     /locus_tag="OO568_000093"
     CDS             complement(93713..94780)
                     /locus_tag="OO568_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004985426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6639605.1"
                     /translation="MNFWGRTVHALSHFPSLFFALLPTSLVWAAPAQRAFSDWQVTCN
                     NQNFCVARNTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGE
                     PLALSGDKWRIPPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFI
                     DAQQKRVGSETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWR
                     MNGLRCSLDPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRL
                     RLPFNNGQETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYA
                     AEPTCDNWHGPDARPTLWITR"
     gene            complement(94881..95456)
                     /gene="nudK"
                     /locus_tag="OO568_000094"
     CDS             complement(94881..95456)
                     /gene="nudK"
                     /locus_tag="OO568_000094"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6639606.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(95524..97503)
                     /gene="aegA"
                     /locus_tag="OO568_000095"
     CDS             complement(95524..97503)
                     /gene="aegA"
                     /locus_tag="OO568_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6639607.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDTPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            97709..99426
                     /gene="narQ"
                     /locus_tag="OO568_000096"
                     /pseudo
     CDS             97709..99426
                     /gene="narQ"
                     /locus_tag="OO568_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            99628..102741
                     /gene="acrD"
                     /locus_tag="OO568_000097"
     CDS             99628..102741
                     /gene="acrD"
                     /locus_tag="OO568_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6639608.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFIHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSADFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNSYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGTQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(102840..102899)
                     /gene="ypfM"
                     /locus_tag="OO568_000098"
     CDS             complement(102840..102899)
                     /gene="ypfM"
                     /locus_tag="OO568_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6639609.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            103280..103636
                     /locus_tag="OO568_000099"
     CDS             103280..103636
                     /locus_tag="OO568_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6639610.1"
                     /translation="MVTLYGIKNCDTIKKTRRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLYAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            103640..104767
                     /gene="dapE"
                     /locus_tag="OO568_000100"
     CDS             103640..104767
                     /gene="dapE"
                     /locus_tag="OO568_000100"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6639611.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            104795..104995
                     /locus_tag="OO568_000101"
     CDS             104795..104995
                     /locus_tag="OO568_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6639612.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(105105..105803)
                     /gene="ypfH"
                     /locus_tag="OO568_000102"
     CDS             complement(105105..105803)
                     /gene="ypfH"
                     /locus_tag="OO568_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6639613.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(105877..107892)
                     /gene="tmcA"
                     /locus_tag="OO568_000103"
     CDS             complement(105877..107892)
                     /gene="tmcA"
                     /locus_tag="OO568_000103"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6639614.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRKLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(107907..108770)
                     /locus_tag="OO568_000104"
     CDS             complement(107907..108770)
                     /locus_tag="OO568_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6639615.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRCSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(108938..109651)
                     /gene="purC"
                     /locus_tag="OO568_000105"
     CDS             complement(108938..109651)
                     /gene="purC"
                     /locus_tag="OO568_000105"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6639616.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVAHRLGVQLD"
     gene            complement(109864..110898)
                     /gene="bamC"
                     /locus_tag="OO568_000106"
     CDS             complement(109864..110898)
                     /gene="bamC"
                     /locus_tag="OO568_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6639617.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPASLEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(110915..111793)
                     /gene="dapA"
                     /locus_tag="OO568_000107"
     CDS             complement(110915..111793)
                     /gene="dapA"
                     /locus_tag="OO568_000107"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6639618.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMARMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            111939..112511
                     /locus_tag="OO568_000108"
     CDS             111939..112511
                     /locus_tag="OO568_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6639619.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            112511..112981
                     /gene="bcp"
                     /locus_tag="OO568_000109"
     CDS             112511..112981
                     /gene="bcp"
                     /locus_tag="OO568_000109"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6639620.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            113234..113851
                     /gene="hyfA"
                     /locus_tag="OO568_000110"
     CDS             113234..113851
                     /gene="hyfA"
                     /locus_tag="OO568_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6639621.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            113851..115868
                     /gene="hyfB"
                     /locus_tag="OO568_000111"
                     /pseudo
     CDS             113851..115868
                     /gene="hyfB"
                     /locus_tag="OO568_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            115879..116826
                     /gene="hyfC"
                     /locus_tag="OO568_000112"
     CDS             115879..116826
                     /gene="hyfC"
                     /locus_tag="OO568_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6639622.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLLTLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGQGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            116843..118282
                     /gene="hyfD"
                     /locus_tag="OO568_000113"
     CDS             116843..118282
                     /gene="hyfD"
                     /locus_tag="OO568_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6639623.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISDYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            118294..118944
                     /gene="hyfE"
                     /locus_tag="OO568_000114"
                     /pseudo
     CDS             118294..118944
                     /gene="hyfE"
                     /locus_tag="OO568_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
     gene            118949..120529
                     /gene="hyfF"
                     /locus_tag="OO568_000115"
     CDS             118949..120529
                     /gene="hyfF"
                     /locus_tag="OO568_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6639624.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            120519..122233
                     /gene="hyfG"
                     /locus_tag="OO568_000116"
                     /pseudo
     CDS             120519..122233
                     /gene="hyfG"
                     /locus_tag="OO568_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            122243..122788
                     /gene="hyfH"
                     /locus_tag="OO568_000117"
     CDS             122243..122788
                     /gene="hyfH"
                     /locus_tag="OO568_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6639625.1"
                     /translation="MLKLLKTIMRAGTPTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            122785..123543
                     /gene="hyfI"
                     /locus_tag="OO568_000118"
     CDS             122785..123543
                     /gene="hyfI"
                     /locus_tag="OO568_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6639626.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            123536..123949
                     /gene="hyfJ"
                     /locus_tag="OO568_000119"
     CDS             123536..123949
                     /gene="hyfJ"
                     /locus_tag="OO568_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6639627.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            123979..125991
                     /gene="hyfR"
                     /locus_tag="OO568_000120"
                     /pseudo
     CDS             123979..125991
                     /gene="hyfR"
                     /locus_tag="OO568_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            126013..126861
                     /locus_tag="OO568_000121"
                     /pseudo
     CDS             126013..126861
                     /locus_tag="OO568_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(126899..127960)
                     /locus_tag="OO568_000122"
     CDS             complement(126899..127960)
                     /locus_tag="OO568_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6639628.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            128173..129636
                     /gene="bepA"
                     /locus_tag="OO568_000123"
     CDS             128173..129636
                     /gene="bepA"
                     /locus_tag="OO568_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6639629.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVLQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLEQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            129657..130016
                     /gene="arsC"
                     /locus_tag="OO568_000124"
     CDS             129657..130016
                     /gene="arsC"
                     /locus_tag="OO568_000124"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6639630.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(130149..130895)
                     /gene="hda"
                     /locus_tag="OO568_000125"
     CDS             complement(130149..130895)
                     /gene="hda"
                     /locus_tag="OO568_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6639631.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(130945..132234)
                     /gene="uraA"
                     /locus_tag="OO568_000126"
     CDS             complement(130945..132234)
                     /gene="uraA"
                     /locus_tag="OO568_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6639632.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(132320..132946)
                     /gene="upp"
                     /locus_tag="OO568_000127"
     CDS             complement(132320..132946)
                     /gene="upp"
                     /locus_tag="OO568_000127"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6639633.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            133272..134309
                     /gene="purM"
                     /locus_tag="OO568_000128"
     CDS             133272..134309
                     /gene="purM"
                     /locus_tag="OO568_000128"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6639634.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGENYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            134309..134947
                     /gene="purN"
                     /locus_tag="OO568_000129"
     CDS             134309..134947
                     /gene="purN"
                     /locus_tag="OO568_000129"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6639635.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQSYAADE"
     gene            135119..137185
                     /gene="ppk1"
                     /locus_tag="OO568_000130"
     CDS             135119..137185
                     /gene="ppk1"
                     /locus_tag="OO568_000130"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6639636.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFGHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            137190..138731
                     /gene="ppx"
                     /locus_tag="OO568_000131"
     CDS             137190..138731
                     /gene="ppx"
                     /locus_tag="OO568_000131"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6639637.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLLRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(138770..>139756)
                     /gene="pdeF"
                     /locus_tag="OO568_000132"
     CDS             complement(138770..>139756)
                     /gene="pdeF"
                     /locus_tag="OO568_000132"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6639638.1"
                     /translation="ALDSHLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELN
                     TVAELSIVTNAPENMQRCGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRG
                     DVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAH
                     RFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQ
                     ELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARM
                     KKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        34771 a        36067 c        34421 g        34497 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaactag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 gcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccaa
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat gcggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtcccgaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactacccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aaacgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagcagc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggtag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccctc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatattgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aatgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata ccccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ccgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgctacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aataagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgag atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatggcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggtgtgatg cccacggtaa aatacagtgc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccaaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag cgtttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtctca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaattacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgcgt cggtattctc ggcggcatgt tgtgtgagac
    23041 gttgaccatg attctggtaa ttgtctgggc accaactacc gcgttgggga tcgatatcgt
    23101 ctctaaaatc ggcattccaa tgatcctcgg tagcgtctgt atcggcttta ttgtgcttct
    23161 ggtgcaaagc gttgagggcg aaaaagaggc cagcgccgcg cggcaggcca agctggcgct
    23221 ggatatcgcc aacaaaacgc taccgctgtt tcgccatgtc aatagcgagt cattacgcaa
    23281 ggtctgcgaa attatccgcg atgacattca cgccgatgcg gtggcgatta ctaataccga
    23341 tcatgtgctg gcctatgttg gcgtgggtga acataactat cagaatggcg atgacttcat
    23401 tagcccgact ccccgtcagg cgatgaatta cggaaaaatc atcattaaaa acaatgatga
    23461 agcccaccgc acaccagaga ttcattccat gctggtgatc ccattgtggg agaaaggggt
    23521 cgtgaccgga acgctgaaaa tttactactg ccacgcgcat cagatcacct cgtcattaca
    23581 ggaaatggcg gtcggtctgt cgcaaatcat ctccacgcaa ctggaggttt cacgcgccga
    23641 gcagctacgt gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca aaattaatcc
    23701 ccattttctg tttaacgctc tgaacgctat ttcatcgtca atccgtctga atccggatac
    23761 cgctcgccag ttgatcttta atctgtcgcg ttatctgcgc tataacattg aattaaaaga
    23821 cgatgagcaa atcgatatca aaaaagagct gtatcaaatt aaagactata ttgccattga
    23881 gcaggcccgc tttggcgaca agctgacggt tatctatgat attgatgaag aggtgaattg
    23941 ctgcattccc agcctgctga tccagccgtt ggtggagaac gccattgtcc acggtattca
    24001 gccttgcaaa ggtaaaggcg ttgtcaccat cagcgttgca gagtgcggaa atcgggtacg
    24061 cattgcggtg cgagataccg ggcacggcat cgatccaaag gtgattgagc gggtcgaagc
    24121 gaatgaaatg ccgggcaata aaattggcct gctgaatgtc catcatcgcg tgaagttatt
    24181 gtatggcgag gggctgcata tccgccgcct ggagccgggg acggaaattg cgttttacat
    24241 tcctaaccaa cgcaccccag tcgcctcaca ggctacgtta ttgctttgag ccggagtgat
    24301 attgtgaaag tcatcattgt tgaagacgaa ttcctggcac aacaggaact gagctggcta
    24361 attaaagagc acagccagat ggagattgtc ggcacctttg acgacggtct ggacgtgttg
    24421 aagtttttgc agcataaccg cgtcgacgcc atttttctgg atatcaatat tccgtcgctg
    24481 gatggcgtgt tgctggcgca aaacatcagc cagttcgccc ataaaccgtt tattgtgttc
    24541 atcaccgcgt ggaaagaaca tgcggtagaa gcgtttgaac tggaggcgtt tgactacatt
    24601 ctcaaaccgt atcaggagtc acgtattacc gggatgctgc aaaaactgga agcggcctgg
    24661 caacaacagc agaccagcag tacgcctgcc gcgacggtaa cgcgtgagaa tgacaccatt
    24721 aatctggtga aagatgagcg aataatcgtc acgccaatta acgatatcta ttacgccgaa
    24781 gcgcacgaga aaatgacctt tgtctatacg cggcgtgaat cctacgtaat gccgatgaac
    24841 attaccgaat tttgcagcaa actgccgccg tcgcattttt tccgctgcca tcgctcattt
    24901 tgtgtcaatc tgaacaaaat acgcgaaatc gaaccgtggt ttaataacac ctacattctg
    24961 cgactgaaag atctggattt tgaagtgccg gtcagccgca gcaaagtgaa agaatttcgc
    25021 cagttaatgc atctttaatc gggaactttc atgaaagcac caggcttgcc cgccgatcaa
    25081 caatttttcg ccgatctgtt cagcggccta gtgcttaacc cgcaactact ggggcgcgtc
    25141 tggtttgcca gccagcctgc ctcgttaccg gtgggcagtt tatgtattga ttttccccgt
    25201 ctggatatcg tgctgcgcgg cgaatacggc aacctgctgg aagcaaagca gcagcgaatg
    25261 gtggaaggag aaatgctgtt tattccggca cgcgcggcta atttaccgat caacaacaaa
    25321 ccggtgatgc tgttaagcct ggtgttcgca ccgacctggc ttgggttatc gttttacgat
    25381 agccgcacca cgtcgttgtt gcatcctgct cgccagatcc agcttcccag cctgcaacgc
    25441 ggtgaaggtg aagcgatgct taccgccctc acccatctta gccgttcgcc gctggagcag
    25501 aatatcattc agccactggt gttaagtttg ctgcatcttt gccgcaacgt ggtgaatatg
    25561 ccgccgggta attcgcaacc gcgcggcgat tttctctatc acagcatttg taactgggtt
    25621 caggataatt atgcccagcc gctcacccgc gagagcgtgg cgcagttttt taatatcacg
    25681 cccaatcatc tgtcaaaact gtttgctcag catggaacga tgcgttttat cgagtatgtg
    25741 cgttgggtgc gaatggcgaa ggcgaggatg attttgcaga aatatcatct gtcaattcat
    25801 gaagtggcac agcgttgcgg ttttccggat agcgactatt tttgtcgcgt tttccggcgt
    25861 cagtttggtc tgacgccggg agagtacagc gcccgttttc agggctaacg tcagaaggtt
    25921 aattctgttt ccagcagcgt caggatactt tgtgcatctt gcgcggcaaa caacgactgg
    25981 cggaagtttt tattcaccag tttacgcgcc agctgcgaga agactttcac atgattcatc
    26041 ccttcgttag cacccagcgt cagcatgatc accagttcga cttcgcccat ttctgactgc
    26101 cagtcaaccg gtttcgccag ccgggcaatg ctgatactgg aatgacggat ccactgagat
    26161 ttggtgtgcg ggatcgctac gccaaaacca acgccggtgg taacaatctc ttcccgctgc
    26221 cagacatctt cttccagctc aaacggatgt tcagtgcgcc cgttaacgcc gaggttgccg
    26281 cacaggaact ggatcgcctg ctctttattg ctaaaatcct gatcaacaaa gatattctcc
    26341 agcgccagca gtgggcgaac gtcttcttcc ggcgtaaagg cggtgagtaa cgcttcaatt
    26401 tcctgcgcac tgcggcattc acatgcctga cgcgccagtt cccgacacgc ctcgctatcc
    26461 agttgacgaa gctggctttt caccgccgga atacgcgggc tactcatact cagctcatcc
    26521 aggcccagcc caagcagtag cggcagataa cggctttcac cgcccagttc accgcaaatg
    26581 cctacccatt tgccccgctg atgcgcagtg gtaactattt gctgcaacat gcgcaggaac
    26641 gatggcgtaa tcgggttata tagcggcgat acgcgcgggt tattacgatc gaccgcatac
    26701 agatactggg tcatatcgtt ggagccgata ctgaagaaat cgacctcatc gcagaagtgg
    26761 tcgatgatgt agcacaccga cggcacttcc accatgatcc caagcgtaat cgtctctgca
    26821 tgacgcaggc catcgcgctt aagctcaacg atcgcttttt ggatctcgcc tttcacccat
    26881 aagatctgat cgaggctgtg aaccatcggg atcatcaact gggcgttgcc gaaactggcg
    26941 gcgcgcaaaa tggcccgcag ttgagtgcgg aacaggccag caaattccgg gtaaatacgt
    27001 accgcgcgat agccgaggaa tgggttctct tcctggggaa cattcagata agaaatgctt
    27061 ttatcgccgc cgatgtccat cgtgcggaag ataatcggct tgtcgcccgc cgccagtagc
    27121 acctgctggt aggcttcaaa ttgctcctgc tcgtccggcg cgctgtcgcg atccatatag
    27181 agcatttcgg tacggaacag cccgacacct tccgcgccgt tggcaaacgc gcctggcgct
    27241 tccagagcgg tgccgatatt cgccgcaata tcaatacgct tgttatcacg ggaataggcc
    27301 agctgcgcgg cagcctgcgc ctgttgtttt tggcgtttat ccgccagcgt ctgcgcgacc
    27361 tgataataac cgctcaccgc gtcattcggg ttaatcgcca gcacgccgca ctgggcgtca
    27421 agcactgcag gttgcccggc ataacgggca atcgcatcca gcggcaagcc actcagtacc
    27481 gggatcgccg aggcacgggc cagaatcagt gtatgcgagg tgcgcccggt tttctccaga
    27541 atcatacccg caagattttt caaatcaagg ctcaaaaact ggcttggggt taaatcttca
    27601 gccaccagaa tggtcggttt ttccagcacc agattgttgc gcggcttcag ttccggccag
    27661 gtgatatgca gcaactgttc gctgatatcg cgaatgtcgc tgacccgctc gcgcagataa
    27721 tcgctggcag aagcagatag tttggcgcaa acctgctcca tattgctgat gatcgccgcc
    27781 cccagtccct gatgctgttc tgtcatcagg cgacggatat tgcctgcaaa ttcatcatcc
    27841 tgaatcagcg acaaatgagc gctgaggata gttttgcttt cgccgtcacg ctcacgcaat
    27901 tgctgattca gttgctcggc aagcgttgcc aggctgtgct ccagccgggt ggaatcttgc
    27961 gcactggcgg ggattgcccg ataactgtcg aggctgtcgc tttgtaacag ggtcagcgta
    28021 cccacgccga cgccgcttgc cagcacattg ccgtacagta aatccgggtt aaggcggctt
    28081 aatgaacgcg gcagcggatg cgccgtcagt tctgcctgcg tcggctgaac gctgtcgctg
    28141 tcgataaagc gcaccaggat gtactcttcc agcacgcgcc gcgcctgctc ttcatcgctg
    28201 ccgctaatgt tcaggctgca actgtcatta aataaggtgc cagttccaat caacgccagc
    28261 gagcttttag catcggcctt tgcgttctgg cgatggttga taaaggtgat ttcgctttgc
    28321 cactggctgc actgttcttt aagttcccgc gccggacgag catgtagacc gttaggcata
    28381 ggcaaaggac agagaaattg aatcgttaac ataaccgctc cgggttaatc aggagatcat
    28441 ctgaaatccg tcagttgaac aaccgtctca cgcgtaagac gttgaataag ggcagataaa
    28501 agttgctcca tctgcaaaat gtcgcggcaa tcggcaatcg atgccgcgca atgtccgtgg
    28561 cgggtggcag gccccattac caatgtgggt acgccagtac cggttaagtg caccgctcca
    28621 ccgtccgtgc cgccgttact gaacatatcc gcctgcaacg gaacgccaat ttctgccgcc
    28681 acggtttcaa tccacgcggt aagttttggc ggcgcaatca gcgatttatc gcttaacacc
    28741 agcatcggcc cgttaccaat ctgacgatgg ttagccgcgc cataatcaaa gtttttcgcc
    28801 cagcaggcgg tatcaagcac aatggcgacg tccggcgaca ccgcgcgggt agcagtttgc
    28861 ccgccgcgta atcccacctc ttcgctggaa cttgccacca gccacacttc cgcaggcagt
    28921 tcggcgtcgt gcagctcacg tagtaacgtc accagcagat agcaaccgag gcgatcatca
    28981 aaggctttgc ccatcacccg ctggtgaggg aggacctgaa aagtggtatc gaaggtgacg
    29041 cgatcgcctg gacgaattcc cgcctgcatc acttcgtcat aggtgcgcgc gccaatgtcc
    29101 acgcgcatgg cgctaacgtc attcccctgc cggtcgccgt caagcaggcc tagaattttg
    29161 cactcttcac gggtggtgat gcgcaccggc tgcagctggc gggcagccat gcgcacgttg
    29221 ccaaccggca gcacgtcaat agcgccttcg cgggagatgc tgcgcaccat aaatcccact
    29281 tcgtccatat gggcgcagat catcaccttc ggacctgtcg attcattgag gcggatcagc
    29341 accgatccca gaccatcaaa tcgcacttct ttttgcaggc gagccgcttc ttccagcagg
    29401 atctgccgca cttcctgttc cgaggaggcg atcgcatctg cctcgctcaa cgcttttaat
    29461 agcgataaat ccattatgcc tctcccgtga gcaacactgt tttcggcatg gcgtagagca
    29521 cttctgcgcc ttgcggggta accagcacaa catcttcgat gcgcacgccc ccttgcccta
    29581 gcaaataaat ccccggctcc acggtcagca acatgcctgg ctgtagcgtc gtggtgtccc
    29641 gcggtgaaaa acgcggatct tcatggactt caatgccgat agcgtgacca gtgttatgac
    29701 cgaaatagtc gccaaatcca gactcggtaa tgacccggcg cgcggcgtcg tcaatctgct
    29761 ggcagcgcac gccggggcga attgcagaga ttgccgcgag ctgtgcctgc aggacaatct
    29821 gatagacgtc aaacagcggg tgagattcgg cgctcacccc ttcgccattc accagcaagg
    29881 tgcgcgtcat atcagcagta gccctgatac agcgcaccaa aatcgagagt gacaaactcg
    29941 cccgctgcaa caatcttgtc gctggctttg ccgtgcggca gcgccccgcg ccagccgctg
    30001 gcgacaatgg tgtcaaaaga ggctttttct gcaccctgct ggcgcataaa ccactccagt
    30061 tcagcggcta tctcgcgctc gctcatcccc gcctgaataa agcggcgaat atgctccgct
    30121 ccgcgatcgg caatcccaca ggcgaggcgg attatctcca cctcctctgg cgttttgatt
    30181 tgccgcagca catccggcgt ggcgctaacc agtttcgcat tgagttcaga cttccagcgg
    30241 tgcgcggttt cccaactcac ctgctggcct tcaaaaccga gcgtttgcaa ctgctcatcg
    30301 gcaatgattt gattgacgat agtggtaagc gtgttcgtcg cgtcaagcaa atgcagctgg
    30361 tagccttgcg cacgggcttc cacctcaacg aaatagcgcg aatctaccag aatgtgcgca
    30421 ctttcacggc taataaccac atagcctgat ccggtggaga tccccagatg cggctgtttg
    30481 ttctgccgtg aggagagaag caccgcatcc agttgttgca ccttaagcca gttgcgcagc
    30541 gaagcgagta atgtcatctg gattttcctt cttacaggct atcgattaac aatttgcctt
    30601 tgcggaacat catcagacgc aggaacacca ccatcagcgc agtaatgatt gcccccagcg
    30661 cgatccccgc catataaacg cccaggttag tgaccagcgg ccatgcccag atagcggatt
    30721 ccgggaacca ttgcactgcg cccagccaga cagctgcggt tgagccgaca atcgcgccga
    30781 ccatatatga cggaatggcg gtgatggggc tttccagcgc aaacggaatc gcaccctcac
    30841 taatccccat aaaggccagg aacatcgcgg tcttaccctg cggataaagt tgagcgttga
    30901 acaggcgttt gccggttaaa cgacggtcaa taatggtcgc cagacccaga ccaatcggcg
    30961 gaataacgat agcgatagaa cgagcggtga ccggtaatac gtggtcagtg gtaaagctga
    31021 aggcaacgaa acctgcggct ttgttgatcg ggccaccaag gtcgattgct gtcgccgcgg
    31081 cgatccccat tgcgtacatc agcgcgccat tctcacctgc ggcagtcagc acagtacgga
    31141 taccgccgtt gatccagcca ccaaaggggg tgatgacgta gtacatcgcc agcatgacaa
    31201 aaatggcgga aagaatcggt agcaaaaatg tggttttgaa cgctaacagg aaatccggta
    31261 actggatttt ctggttcatc catttcacaa ggtaaccggc aacgatagaa ataatcagcg
    31321 caccaatgaa ggtggacggc accggcgaag aggtcgccca ttgcatagtg ctgggatcga
    31381 agttcagcag ttgcgtcggc tgggtagaca tcaacccgcc gataaaacca gccgggaacg
    31441 ccagtttgcc accgatagag ttcgccacaa atgcagcaaa catcggaatg gcaaaaccaa
    31501 acaacacgcc gccgaaggac tgcgacagcc aggcaaattt aagtaaggag aggtcgaaac
    31561 cggagaattt cccgctatta agagcatcca tgatgccgat ctcagcagga attttaagcc
    31621 agctataagc aatcagctgg ctgaaagcga ggatcacacc gcccataatt aaggtcggca
    31681 ccatgcgtga aatccctgac atcacatgct gcgggagttc accccagaag ctgcttttag
    31741 aggcctgcgg attcttaact gccgctgccg caccggatgc gccaggcaca acggttgcac
    31801 tgcgtttttt aatggccata acaattatcc ctgtcgaata ttactgttgt tcagaggcaa
    31861 tcatctcttc gatttctttg atgatgcccg cagcgttttt aattgcgtcc tgcaaagtga
    31921 tttcataaac gtcgcgtgat tcgaaacgtt cgttatcttc cggggtaact gccacggaat
    31981 ggatgatgat ggtcgcttcg gcgatatcct gcgccgtcag gcgattctgt atgccgtccg
    32041 cgccctgggt ttcaattttc acttcataac cggcttctac cgccgcttct tccagcgcct
    32101 gagcggccat aaaagtgtga gccaggccca ttgggcaggc acataaggca atcagtttct
    32161 tactcatcgt taattcctcg ttcagtgata gttgacgagt gaaattatga acgtgcaaaa
    32221 caaatcgccg ttaccagaca aataatgcat ttactggaga attaatcccg ctgtacggaa
    32281 gtgtgacgca ggtcggcctt gtgaggggac acaaagaaaa attgaattaa ctgtatgatt
    32341 taaaagatta tcgggagagt tacctcccga tataacagga aggatttaca gaatgtgacc
    32401 taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggatagtcat ggacgatgag
    32461 atacaccgga atatcatgga catattcttt aaagcgccct ttatcttcaa atgcggcacg
    32521 gaaaccggag gctttgaaga actcaaggaa gcgcggcacg ataccgcccg caataaacac
    32581 gccgccaaat gtcccgagat tgagcgccag attgccgcca aaacggccca taatgacgca
    32641 aaacagcgac aatgcgcggc ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat
    32701 atcttttggc ttgagatttt ctggcaggcg gttgtcagct ttcacaattg cgcgatacaa
    32761 attcaccagc ccagggccag aaagcacgcg ctccgccgaa acatgaccaa tttccgcacg
    32821 caatatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc
    32881 ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc
    32941 cgttccggca ccgtaaaccg caataggctt accttcgacc ggttctgcgc caccaaactg
    33001 aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa aatcgttaat
    33061 aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt
    33121 atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc
    33181 tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta
    33241 atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc
    33301 aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact
    33361 gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca
    33421 acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacaatta ctttatcgtt
    33481 tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc
    33541 ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg
    33601 ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg
    33661 tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc caggattccc
    33721 ccctctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt
    33781 tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccggttc
    33841 cgccctctgc gctacctgga cttatcgtag cattaattct cggtcttgct ggcggcgtca
    33901 gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgccgtc gccattggcg
    33961 ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca
    34021 tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg
    34081 gcagtagtga agttccgcta tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg
    34141 gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg
    34201 gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag
    34261 cagggatggt cgccgtatgg tgcttaccac ggttgcacgc gataatgaat caaatgaaaa
    34321 atccggtgct cgtgctgggt attggcggat ttattctcgg aattctgggg gttattggtg
    34381 gaccagtttc gctgtttaaa gggctggatg agatgcagca gatggtggca aatcaggctt
    34441 tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg gtcgttgctg
    34501 ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc gtggcattag
    34561 ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt tcctgcgcta
    34621 ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt atggcggcag
    34681 tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc
    34741 tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg
    34801 ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga tgatacgggg
    34861 ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa
    34921 taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc ccgcttgcag
    34981 caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc gatcgcgatc
    35041 attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg catcgcaatt
    35101 atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt ggctgggata
    35161 agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg
    35221 gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag cacaatgcct
    35281 gatgcgacgc tcattcaact acaatcccag cgccgtcccc accaacaacc agatattcag
    35341 cgccacgacc agcactacaa tcacccagcc tgtctgtttt acgcgtttgc tgttcaccag
    35401 atcgcccatc aacttgctgt cactggtgaa aatcagcagt ggaaccagcg ccagggcgat
    35461 accaaaactt aacagcacct gactcataac cagaatccgt gtcggatcta atcccatcag
    35521 aatgacaata aatgacggca acatggtgac tgtacgacgc acccacagcg ggatatggaa
    35581 gcgaatgaat ccctgcatca ccacctgccc cgccagtgtc cccaccaccg ttgaggacag
    35641 tccggcagca accagactta acccaaagac cgttgccgca gcatggctta acagcggttg
    35701 cagcgtcaga taagcctcat caagatcggc aacaccagta tgaccagaaa agtggaacgc
    35761 cgccgcagct gtagccatca tcgccagatt gacaaaaccg gcaatcgtca tggcgatagc
    35821 cacatcccat ttggtggcgg aataacgttg ttgacgcgaa ccgccatgta aatgctgagt
    35881 gagcgaagag tgcaaataaa tcacatgcgg cataatcgtc gcccctaaca cgcctgctgc
    35941 caggaagacc gcctccgaag taggtaaact cgggatcacc attcctttac ccagctgcgc
    36001 caggttaggc tgggagaaaa tcaactcgac aatgtaagcc gcggcaacaa acaacagtaa
    36061 cccgccaatc actttctcca gcggtttttg cccgcgacgt tgcagcatta aaatcaggaa
    36121 agtcgcgatc cccgtcagca ccgcgccctg caacaacgaa acaccaagaa tgagtttaaa
    36181 accgatcgcc gcaccaataa attccgccag gtcggttgcc atcgcaataa tttctgcctg
    36241 aacccaatag aaccacacta cgggacgcgg atagtgatca cgaatctgct ccgccagatt
    36301 tttaccggtg gcaatcccta gtttggcaga gaggatctga attagcatcg ccatcaggtt
    36361 ggcccaaacg acaacccaca gtagctgata gccgaagcta gcacccgcct gaatattggt
    36421 cgcaaagtta ccgggatcga tataaccaat cgccgcaatg aacgcaggtc ccattaatgc
    36481 gagcctcatc ttgcgcgccg cccgtccgct gctactctca acgcgatagt tcgtcatctt
    36541 gtgcctctaa aacatagcct ttgctatgtt tcatgctatg ctaaacgaga atgattatca
    36601 aattcattta aatggattgt ggtgatttct ctgatagacc aagattatga ttacgaaaag
    36661 attgatgact tgaatgttat ggttatgttt aatgttagca catttacata actttcagct
    36721 tccatacaca acatagcaga aatgtatgac agatcactat ttttgaagcc tgtcacagga
    36781 cgtcattata gtgtgtgtca gatctcgttt tccttaacca tgttacatag aatgtgcacg
    36841 gaaatttaac ctgcctcata tttggagcaa atatggaccg cgtccttcat tttgtactgg
    36901 cacttgccgt tgttgcgatt ctcgcactgc tggtaagcag cgaccgcaaa aaaattcgta
    36961 tccgttatgt tattcaactg cttgttatcg aagtgttact ggcgtggttc ttcctgaact
    37021 ccgacgttgg tttaggcttc gtgaaaggct tctccgaaat gttcgaaaaa ctactcggat
    37081 ttgccaacga agggactaac ttcgtctttg gtagcatgaa tgatcaaggc ctggcattct
    37141 tcttcctgaa agtgctgtgc ccaatcgtct ttatctctgc actgatcggt attctccagc
    37201 acattcgcgt gttgccggtg attatccgcg caattggttt cctgctctcc aaagtcaacg
    37261 gtatgggcaa actggaatcc tttaacgccg tcagctccct gattctgggt cagtcagaaa
    37321 actttattgc ctataaagat atcctcggca aaatctcccg taatcgtatg tacaccatgg
    37381 ctgccacggc aatgtccacc gtgtcgatgt ccatcgttgg tgcatacatg accatgctgg
    37441 aaccgaaata cgtcgttgct gcactggtac tgaacatgtt cagcaccttt atcgtgctgt
    37501 cgctgatcaa tccttaccgt gttgatgcca gtgaagaaaa catccagatg tccaacctgc
    37561 acgaaggtca gagcttcttc gaaatgctgg gtgaatacat tctggcaggt ttcaaagttg
    37621 ccattatcgt tgccgcgatg ctgatcggct ttatcgccct gatcgccgcg ctgaacgcac
    37681 tgtttgccac cgttactggc tggtttggct acagcatctc cttccagggc atcctgggct
    37741 acatcttcta tccgattgca tgggtgatgg gtgttccttc cagtgaagca ctgcaagtgg
    37801 gcagtatcat ggcgaccaaa ctggtttcca acgagttcgt tgcgatgatg gatctgcaga
    37861 aaattgcttc cacgctctct ccgcgtgctg aaggcatcat ctctgtgttc ctggtttcct
    37921 tcgctaactt ctcttcaatc gggattatcg caggtgcagt taaaggcctg aatgaagagc
    37981 aaggtaacgt ggtttctcgc ttcggtctga aactggttta cggctctacc ctggtgagtg
    38041 tgctgtctgc gtcaatcgca gcactggtgc tgtaaaacca tacataaaaa agccggggat
    38101 cattccccgg cttttttatg cctgttaatc agctaatggc tgcgggcgac caatcaaata
    38161 cccttgcaga tattgcaccc cgagcttatg caatagcgcc tgctgctgtt gcgtctcgac
    38221 aaactccgcg accacactca atgacttcgc tttcgccaga tcggtaattg atctcacaat
    38281 catcgcatcc agcgtattcg tgacaatatc tttcacaaag acgccatcaa ttttgatgat
    38341 atcagcctgc aaacgcttta accgttcgta gttggcatat ccggcgccaa agtcatcaat
    38401 cgcaatccgg aaaccaaact tatgcagctg ctcgatgttg tacatgctgc tttctgcgtt
    38461 agaaaacgcc tgctcctcgg tgatctcaag aatgaccgcc tgcggggaga tgtgataacg
    38521 cttaaacaga cgaataatcc gcccggcaat attcttttgc agcagcgtga gcggcattaa
    38581 attgactgaa aagcgcggtc cttttttgtc gcaagggtgt gttgccagcc acttcaacaa
    38641 ggattccagc acttgcaaat caaaacgcgc gctaaggtta aactgagcaa taaggggcag
    38701 aaacttatcc ggggtcataa tgccgccgtc atatttcagt cgcgcgagga tctcatcata
    38761 accttcaccc tctttgttgc gaattggctg ggcgtagagc agcaaatcac cctgatctaa
    38821 cgccgtgcga atggtattca gcaatagcac ctgtttagtg gtctgcccgg aaaccatctc
    38881 ctctctgcta tccagcgcca gcacatgatg atgtgcgcag gattgctccg ccagccagct
    38941 taactgcccc aacaagggtt gcagggtttc ctgatttcca tcaaaacgcc cccaggcagc
    39001 accatagccc atatccagcc cggtattgtt ccagtgaatt tgccgactat tcaggatgtt
    39061 aaccatatgc tggagtcgcc cttccgtttc cggcccgctc agcactaaca gcagttcact
    39121 acccggcaat tgatacaact tttcgttttc ctgcatcaac ggcagcagcg tacggcaaat
    39181 tgagcggata cagtgaacgc gcatcattaa gccgtaatga cgactcataa actcaagatt
    39241 atcaatgcgc aggcagcaaa aactcttgcc cgcctcttgc tccggcgctt gttccaacgc
    39301 acgaaagttg ggtaggagcg ttaacggatc tgttagcgcc tgcaaatgcc agcggcgatt
    39361 aagccattca ctgcgatgat aaatgcgcac catatagagc aggcaaacgc taaaggaaat
    39421 cagcaccgcg agaataaatg ccagcgaata ttcggtttcc accccttgca aaaagttctg
    39481 gttgtaattc agaaggcaaa gcgttgaaac agcctaggtg agatttaaaa acggatagcg
    39541 aagattaccg accccgaggg taaagatgat gaagaaaacg ggtaccaggt aaccggcaat
    39601 aaagtcgttt tcataaggtg tgcacaacag aagtaggagc acgcttaacg ctgccagcca
    39661 gcttaaggta aatgcgcgtt tctctttgcc caacgacgga gcgatatccc tgcgccacaa
    39721 tatctgcgca aagtggggac ttacaatcat gcgagtgaga tagtagaaaa gcatgttgta
    39781 aatcagcact gcggtgaaaa ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc
    39841 gccgaaaaag gtggatatct ttagcgggaa atcaaagaag ctccccacaa gatacatgct
    39901 gcatttgatg ccgatcggcg tcaccaaacc aagccagaac agacgttgcc agacatatcg
    39961 gctggtcaat ccataacgcc aacgcgtccc cagctgccag cgtaatattg cgcaggcaca
    40021 caacacgaca aacgtctggc tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa
    40081 gttccacaga ttggtgcaaa acatcccgca taaaacggga acgacaccgc gccagccaaa
    40141 gataaacaag attgacagca ttacgcacag tggcatccac gccagaaaga tataactgga
    40201 atggataatg gcaagtggcg aaataaaacg ggatagctga ataagtacca cggtgagcgt
    40261 aaacgctagt gtgaatatct tgatattttt tatcaggtta tgctccacaa acatgagtac
    40321 actactctca ctgatcgggg ttttcaggca cacaaataat aagtttattc gcatcgcgaa
    40381 gcaaacaggg gccagcctgt tgccgatttt tcatccaatg atgctttcac caggcggcgc
    40441 taacccggac gtaaactgta ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct
    40501 ttgcagcgag ggttggaaat ttggtggagc taagcgggat cgaaccgctg acctcttgca
    40561 tgccatgcaa gcgctctccc agctgagcta tagccccacg atgcgtttac gtaccaaatt
    40621 tgctgggtgc aaaatttggt ggagctaagc gggatcgaac cgctgacctc ttgcatgcca
    40681 tgcaagcgct ctcccagctg agctatagcc ccgtcacgta aagcttgtcg agttgacggg
    40741 cggcatcata tgaattccgc ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat
    40801 cgctgaaaaa tcaggcaaat gaacaatttt ggaaaaccgc tcgcattcag tagttattca
    40861 tgtcacggtt tcctgtaaag tggtgttata aaatgaacta ctaatagacc cacatacatt
    40921 cagggaattg ttatgtccaa ggagaggatg acaccagatg aacttgccag actgaccggc
    40981 tatagccgcc agaccattaa taaatgggta cgcaaggaag gctggacaac gtcaccaaaa
    41041 cctggcgtcc agggtggcaa agcccgactg gttcacgtca atgaacaggt tcgtgaatat
    41101 attcgcaatg ctgaacgtcc agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg
    41161 ccgcttgaag tattgctggt gacactggcg aaagagatga cgccagttga acaaaaacag
    41221 tttacatcct tgcttctgcg ggaagggatt atcggattgt tacaacgctt agggattcgc
    41281 gatagcaaat aatatgaaaa gattacgcaa taaaatgacc accgaagaac tggctgaatg
    41341 cctcggtgtg gctaaacaaa ctgttaaccg ttggatcaga gaaaaaggct ggaaaacgga
    41401 aaaatttccc ggcgtgaaag gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg
    41461 cgagtttatt cagaacacgc cagccttcca taacacacca atgttgatgg aagctgaaga
    41521 gagaattgct gaatacgctc ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc
    41581 gatagataat atgacagaca ttgagcagga aaaagtcgcg caatttttgt cacgtgaagg
    41641 aattcgcaat ttcctcgccc gtctcgatat tgacgaatca gcataaataa aaaacggcag
    41701 gatattatct cctgccgttt atctttttac acgctaatta ctgctgattt tcgcgttcag
    41761 caataaaatc cagcgctttg ttgatacgct cgatactgcg ggtcttacca attgcgtgaa
    41821 cggtaacatc cagtgctgga gactgccccg caccggttac ggcgacacgc agcggcatac
    41881 caactttacc catacccact tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt
    41941 tttcagcggt ccagtcagta atcgcggcca gtttgtcacg aaccacttcc agcggctgac
    42001 gcgctaccgg acgcagatgt tttttcgccg catcggcatc gaactcagca aaatcttcgt
    42061 agaaataacg gcagctctgt gccatctctt tcagcgtctt gcagcgttcg cccagcagtt
    42121 tcaccagatc agccagctgc gggccgttac gggtatcgat attttcctgc tcaatgtgcc
    42181 actgtaagtg agtagcaaca tactccggcg gcagcgcgtt aatgtagtga tggttcagcc
    42241 acagcagctt gtcggtgttg aacgcactgg cagatttgct gacggcattc aaagtgaagt
    42301 atttgatcat ctcttcacga gtgaagattt cctgatcgcc gtgggaccag cccagacgca
    42361 ccagatagtt cagtagtgct tctggcaaat aaccgtcatc acgatactgc attacgctga
    42421 ctgccccgtg acgtttggac agttttttac cgtcatcgcc attgatcata gaaacgtgcg
    42481 cgtaaaccgg caccggcgct ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt
    42541 tgatatggtc ttcgccacgg ataacgtggg tgatttccat atcccagtca tcgacaacca
    42601 cacagaagtt ataggttggg gaaccatcgg tacggcggat aataagatcg tccagttcct
    42661 ggttgctgaa ctcgatcgga ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg
    42721 ggttagcaaa acgtacaaca cacggttcat catcagcatg atgctcatgg ctgtggcggc
    42781 agcgaccgtc ataacgcggc ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca
    42841 ggcgctcttt agagcaatag catttataag cagtgccctc ttccagcatc tgatcgatca
    42901 ccgcgttgta gcgatcaaaa cgtttggtct ggtagtacgg accttcatcc cactccaggc
    42961 tcagccagtt catgccatcc ataatggctt cgatagcttc cggcgtggaa cgctcaagat
    43021 cggtgtcttc aatacgcagc acgaactcac cgccgtggtt acgtgcaaaa agccaggagt
    43081 aaagagcagt acgcgcgccg ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag
    43141 ttttgatttt catgaaatgg ccttacgttt agaaagatgc cgacaaccgg caaatcctgg
    43201 aaaaattgag tgggcgatat tctatcactc ccgcctgatt cctcaatgta gttcgggttt
    43261 ctaccttacg cattattgtt ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca
    43321 tcttgcttaa atttacgacg aacgaacaat ttctttagaa aatgcgttga ctcattttga
    43381 actctcccta taatgcgact ccacacagcg ggggtgatta gctcagctgg gagagcacct
    43441 cccttacaag gaggcggtcg gcggttcgat cccgtcatca cccaccaact actttatgta
    43501 gtctccgccg tgtagcaaga aattgagaag tgggtgatta gctcagctgg gagagcacct
    43561 cccttacaag gagggggtcg gcggttcgat cccgtcatca cccaccactt cgggtcgtta
    43621 gctcagttgg tagagcagtt gacttttaat caattggtcg caggttcgaa tcctgcacga
    43681 cccaccaatg taaaaaagcg ccctaaaggc gcttttttac tatctgcgat actcaaagat
    43741 tcgaacctgc agcaggtttg agttgagcgc agcgaaacaa cggagccgct cgcggcgacg
    43801 gcccgaaggg cgagcgaagc gagtcatcct gcacgaccca ccaatgcaaa aaagcgccct
    43861 aaaggcgctt ttttgctatc tgcgatttgt aagattgatt tttcttctca agttccttgt
    43921 tcttctaccg atattacctc gtacagtgac gaggactgaa caatggccac tatctcttca
    43981 acttcaattc cttccataca aacacagagt agtaacaggg catcacaagg tagcgatgtt
    44041 gcttcacaaa tcgcgcggat aagtcagcaa atcatcaaac tcactcaaca aattaaagag
    44101 attgtagaca ctagcggcag tgctgaagac aagcaaaaac aggccgaatt gattcaacag
    44161 caaatcaccc tactggaaac acaactggcg cagttgcaga aacagcaggc agaaaaggcg
    44221 caggagaaag agcagcgttt gtctctgaat gtatcccttc taaatcctgt tgaaaaaacg
    44281 actcatatcg atatctatat ctgatacaac ggatcccctt cccgaccagc cattagcgca
    44341 ttgccctggt ctcgtaccac gtcccataac gcttcagcgg cggtggaaag tgaacggttc
    44401 ttcctgcgca ccagcatcaa ctggcgttca accaccggcg taatgcgctt aaccaccagt
    44461 gggctacctt caggcaatgg tagcgccagt gcaggaagaa tactgatgcc gatgcctgcg
    44521 gctaccatcg ggaacagcgt cgccggatgt ccaatctcct gcacaatatt cgcctgaata
    44581 ccattgcgcg ccagcgctgc gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac
    44641 accaatttcg ctccctgtaa tgcctgccag ggtacataat cttccacggc caaagcgcta
    44701 tcgcgatggc aaagcagaaa gaaaggctcg gaaagaatcg cttcacattg cagatcgccc
    44761 actgggcctg gatcgatgac gatgccaaag tcgacatctc cctggcgaat actttccatc
    44821 acccactgct gcggacgatc atgcaggaca aactgaatat ctggatagcg gcgatggctt
    44881 tcagcaatgc attgcggaat aagatgcgcg gaaatggtct ggctggcagc gacccgaact
    44941 tttccgctca gttgttgccc catacgcccg gtatcgcgca acgtgctgtt aagttcatcc
    45001 agcagtcgct caagacgcaa agccaactgc tgccctgcat ctgtaagcac cacttcacgc
    45061 gtggttctgt ccagcaggcg aacaccagta tgattttcca gctccttcac actgtgactt
    45121 actgccgact ggctcaagcc gatacgctct cctgcacgac taaaactttt ctcctgcgct
    45181 actgtgacga aaacctttaa ttgttttaaa gaataattca tctgttttct tcatgaatgg
    45241 atgcaataaa tcaattttat ttctcaaact ggaagaagca caatagaacc atcgatcatc
    45301 tggagtcttt atgaaacttt ttcgtatcct cgatcctttc accttaaccc tgatcacggt
    45361 ggtgttgctg gcctctttct ttccggccag aggcgatttc gtccccttct ttgaaaatct
    45421 gaccaccgca gctattgccc tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc
    45481 gattattgct ggcggtggtc actggcgact gcatttgtgg gtaatgtgca gcaccttcgt
    45541 gctgtttccg attctgggtg tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat
    45601 gctctactcc ggttttctct acttgtgcat tctcccggct accgtgcagt ctgcaatcgc
    45661 cttcacgtca atggcgggcg gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag
    45721 cctgctgggg attttccttt caccattgct ggttggtctg gtgatgaacg ttcacggtgc
    45781 aaggggcagc cttgagcagg tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt
    45841 ggggcatctt tcccggccgt ggattggtga ctgggtgtcg cgcaataaaa aatggattgc
    45901 gaaaactgac cagacgtcca ttctgttggt ggtttataca gcgttcagcg aagccgtcgt
    45961 taatggtatc tggcataaag ttggctgggg atcattgctg tttatcgtgg tggtcagctg
    46021 cgttcttctg gctatcgtga ttgtagttaa cgtctttatg gcacgccgac tgggcttcaa
    46081 taaggcagat gaaattacta tcgtcttttg tggttcgaaa aagagtctgg caaatggcat
    46141 cccgatggca aacattctgt tccccacatc ggtgatcggt atgatggtgc tgcccctgat
    46201 gattttccat cagatccaat tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca
    46261 gaccgaacag ttacaggcgc agcaggaaag cagcgccgat aaagcttaaa gcggacgctt
    46321 caggggctgg accagttgcg tcagcccctc ggttttaatc agcagcgtga tttgcattag
    46381 ctcaccgagc ttccctgccg ggaactcatc tttgcgggca aaccacagca gatactcctc
    46441 cggcaggtca attaagcgac gccctttgta tttgccaaac ggcattatcg tattggctat
    46501 ttcaatcagc tgctcttttt ccatctcagc tacccagcaa acgcagcatt tccgcttcgt
    46561 cgatgacttc aatgcccagt tcctgcgcct tcgccagttt agatcctgca gcttcacccg
    46621 ctatcaccag atcggttttc ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca
    46681 gtcgagcttt agcgtcatca cgcgacatct ggcttaagct gcccgtaagc accacggttt
    46741 taccagcaaa cgggctgtca atctcttccg cgttgataac gatcggcgca ggccagtgaa
    46801 caccttccgc caacagctcg ctgatgacat tgcggttgct ttcttcggca aagaagttgt
    46861 gaacgtggga tgcaacgaca atgccaacat caggcacctt ttgcagctct tcaatcgaag
    46921 cggcttccag cgcttccagc gtgccgaaat atgccgccag acctgctgcg gtggcctcgc
    46981 cgacttcacg gatgccaagt gcatagagga agcgagcaaa ggtggtttct ttcgcttttt
    47041 ccagcgcgtt aaccacgttt tgtgccaatt ttggccccat acgctccagt ccggtcagtt
    47101 tgcctgcggt gagtttgaac agatctgccg gagtatggac atattctttt tcaaccagct
    47161 gatcgatgat tttgtcgccc attccgtcaa catccatcgc acggcgggaa acaaagtgtt
    47221 tcagcgactc tttacgctgc gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt
    47281 caccttccac acgctcaacg tcagaaccac ataccggaca atgcgtcggg aatacaacct
    47341 cacgggtatc ttccgggcgt tcagaaagca cgacgttaac cacctgcggg atcacgtcgc
    47401 cagcgcggcg aatcaccact ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg
    47461 cattgtgtaa ggttgcgtta ctcaccagca cgccagcaac atggacaggt tccagacgcg
    47521 caacaggcgt aatcgcgcca gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca
    47581 tctgctcctg cgccgggaat ttaaacgcta ccgcccagcg cggggcacgc gcgacaaagc
    47641 caagctgctc ctgctgttcc agtgagttga ccttaatcac cacgccgtcg atatcaaagc
    47701 ccagcgtcgg gcggtcttct tccactttgt gatagaacgc cagcacttct tccgccgatt
    47761 cacaaagcgt tacccgatcg ctgaccggca acccccactt tttaaattgc agtaaacggc
    47821 caagatgagt atccggcagc tcgccacctt ccagaacacc aacgccatag cagaaaaaag
    47881 tgagcggccg cttcgctgta atacgcggat caagctgacg cagtgaacca gctgccgcat
    47941 tacgtgggtt agcaaaaact ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga
    48001 accccgcctg cggcaggaac acttcaccac gcacttccag acgcgccggg atattctctc
    48061 cgtgcagctt cagcggaatg gcgcgaatag tacgcacatt agacgtgata tcttccccgg
    48121 tggagccatc gccacgggtc gcggcactga ctaaaacgcc attttcatac agaatactga
    48181 cggcaaggcc atccagcttc agctcacagc accaggtgac tttctcgttg tttttcagac
    48241 ggtcctgcac acgtttgttg aaagcaagaa agctttcttc atcaaaaacg ttatccagtg
    48301 acagcattgg tacttcatgg cgtatctggc tgaaagctgc cagcggcgca gcacctacac
    48361 gttgggtagg cgaatcaggc gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt
    48421 cgcgcatcag cctgtcgtat tcagcgtcgg gaatttccgg cgcatccatc acatgataaa
    48481 gatattcatg atggcaaagc gtcgttcgca gttctgtcag ttgttgttcg attgattcca
    48541 tatcgcacca tcaatgctaa aaacccccga caagcggggg ttcgaagagg agttaatttg
    48601 ccttaagtgt atcaggcgtt ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc
    48661 aatttctgcg gagtcatcat acggcgctga tcgtcaagca cgacaccgcc cacttcatcg
    48721 gcaatatgct gcgcagattg cagcatcagc ttgaagttct gcagctcgtc accgtaagac
    48781 ggtacctgca taaagatagt gacacccgga gtagtgaaat ccttcatttc aggatcaaag
    48841 gttcccggtt tcaccatatt cgccaggctg aataacgccg ggccgctgcc atccgggcta
    48901 agatgacgat ggtaaatatt catatcgcca aaaatgaagc ccgcttgttg aatgctgtta
    48961 agaagcagtt caccgtttag ctcgctaccg tgatgcgccg cgacgttcat gataatcacc
    49021 gcttctttgc gcttcggttt atccataact ggagccggtt ccgctacagg ctcaggctgt
    49081 ggtgccgcta cgggttctgc aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc
    49141 actggctgcg gcgctggcgc gacctgtggc gaaactggct gctggaccgg ttgacgcggc
    49201 tgcgcagacg cataaggcgg ttggtactgg tgttgcggcg acggacgagc agcctcatgc
    49261 tcctgagcgt tagccggggc atgattcacg cggtgaacac gaacctcacc aacgccctca
    49321 tcatcttcga catcctcgtc ataagaatcg tcgtcacgtt ttgacttcat tcgttttaat
    49381 ggccgatcgc ggaacataga agatcgttct ttacggctgg tccagaaacc atgtaccagt
    49441 aaagcgatta tggcgatcgc gccaacaatg attaatatca gacgcaaatc ctgcatcatt
    49501 atattctctg ttgttctaac accttgccac cacggcaaac atttactcac taagagtatt
    49561 tgccgattac ctcaagtgca agtgcactat taactttcac agcacaaaga tagatgaaat
    49621 cgtgcttttt gctgtttttt cgaacatatc ctaactgtcc attgcgcaat tacccggtaa
    49681 aatacgcaga attttcctgg gattggtcaa aaggagctca tcctgattat ggtttcatca
    49741 ttcacatctg ccccacgcag cggtttttac tattttgcgc aaggctggaa gctcgtctcg
    49801 caacctggga ttcggcgttt cgttatttta ccgctgctgg tcaatatttt gttgatgggg
    49861 ggcgcattct ggtggctctt tacacagctc gatgtctgga tcccgacttt catgagttac
    49921 gttccggact ggctacaatg gctgagttat ctgttgtggc ctctggctgt catctctgtg
    49981 ctgttagtgt ttggctattt cttctccacg attgctaact ggattgccgc tccgtttaac
    50041 ggtttattgg ctgaacaact ggaagcacga ttgactggcg ctacaccgcc agataccggg
    50101 attttcggta tcatgaaaga tgtgccgcga atcatgaaac gcgaatggca aaaatttgtc
    50161 tggtatctgc cgcgcgcaat tgtattacta attctttact tcatccctgg tattgggcaa
    50221 accgtcgcgc cggtactgtg gttcctgttt agcgcctgga tgttagccat ccagtattgc
    50281 gattacccct tcgataacca caaagtgccg tttaaagaga tgcgcaccgc cctgcgcaca
    50341 cgcaaaatca ccaatatgca gtttggtgct ttaaccagcc tgtttacgat gatcccgctg
    50401 cttaatctgt tcatcatgcc cgttgccgtt tgtggcgcga cggcgatgtg ggtcgattgc
    50461 tatcgcgata aacacgcgat gtggcggtaa caatctaccg gttattttgt aaaccgtttg
    50521 tgtgaaacag gggtggctta tgccgcccct tattccatct tgcatgtcat tatttccctt
    50581 ctgtatatag atatgctaaa tccttacttc cgcatattct ctgagcgggt atgctacctg
    50641 ttgtatccca atttcataca gttaaggaca ggccatgagt aagatttttg aagataactc
    50701 gctgactatc ggtcacacgc cgctggttcg cctgaatcgc atcggtaacg gacgcattct
    50761 ggcgaaggtg gaatctcgta accccagctt cagcgttaag tgccgtatcg gtgccaacat
    50821 gatttgggat gccgaaaagc gcggcgtgct gaaaccaggc gttgaactgg ttgaaccgac
    50881 cagcggtaat accgggattg cactggccta tgtagctgcc gctcgcggtt acaaactcac
    50941 cctgaccatg ccagaaacca tgagtattga acgccgcaag ctgctgaaag cgttaggtgc
    51001 aaacctggtg ctgacggaag gtgctaaagg catgaaaggc gcaatccaaa aagcagaaga
    51061 aattgtcgcc agcaatccag agaaatacct gctgctgcaa caattcagca atccggcaaa
    51121 ccctgaaatt cacgaaaaaa ccaccggtcc ggagatctgg gaagataccg acggtcaggt
    51181 tgatgtattt attgctggcg ttgggactgg cggtacgctg actggcgtca gccgctacat
    51241 taaaggcacc aaaggcaaga ccgatcttat ctctgtcgcc gttgagccaa ccgattctcc
    51301 agttatcgcc caggtgctgg caggtgaaga gattaaacct ggcccgcata aaattcaggg
    51361 tattggcgct ggttttatcc cggctaacct cgatctcaag ctggtcgata aagtcattgg
    51421 catcaccaat gaagaagcca tttctaccgc ccgtcgtcta atggaagaag aaggtattct
    51481 tgcaggtatt tcttctggag cagctgttgc cgcagcgttg aaactacaag aagatgaaag
    51541 ctttaccaac aagaatattg tggttattct accatcatcg ggtgagcgtt atttaagcac
    51601 cgcattgttt gccgatctct tcactgagaa agaattgcaa cagtaatgcc agcttgttaa
    51661 aaatgcgtaa aaaagcacct ttttaggtgc ttttttgtgg cctgcttcaa actttcgccc
    51721 ctcctggcat tgattcagcc tgtcggaact ggtatttaac cagactaatt attttgatgc
    51781 gcgaaattaa tcgttacagg aaaagccaaa gctgaatcga ttttatgatt tggttcaatt
    51841 cttcctttag cggcataatg tttaatgacg tacgaaacgt cagcggtcaa cacccgccag
    51901 caatggactg tattgcgctc ttcgtgcgtc gcgtctgtta aaaactggcg ctaacaatac
    51961 aggctaaagt cgaaccgcca ggctagactt tagttccaca acactaaacc tataagttgg
    52021 ggaaatacaa tgttccagca agaagttacc attaccgctc cgaacggtct gcacacccgc
    52081 cctgctgccc agtttgtaaa agaagctaag ggcttcactt ctgaaattac tgtgacttcc
    52141 aacggcaaaa gcgccagcgc gaaaagcctg tttaaactgc agactctggg cctgactcaa
    52201 ggtaccgttg tgactatctc cgcagaaggc gaagacgagc agaaagcggt tgaacatctg
    52261 gttaaactga tggcggaact cgagtaattt cccgggttct tttaaaaatc agtcacaagt
    52321 aaggtagggt tatgatttca ggcattttag catccccggg tatcgctttc ggtaaagctc
    52381 tgcttctgaa agaagacgaa attgtcattg accggaaaaa aatttctgcc gaccaggttg
    52441 ctcaggaagt tgaacgtttt ctgagcggtc gtgccaaggc atcagcccag ctggaaacga
    52501 tcaaaacgaa agctggtgaa acgttcggtg aagaaaaaga agccatcttt gaagggcata
    52561 ttatgctgct cgaagatgag gagctggagc aggaaatcat agccctgatt aaagataaac
    52621 acatgacagc tgacgcagct gctcatgaag ttatcgaagg tcaggcttct gccctggaag
    52681 agctggatga tgaatacctg aaagaacgtg cggctgacgt acgtgatatc ggtaagcgcc
    52741 tgctgcgcaa catcctgggc ctgaagatta tcgacctgag cgccattcag gatgaagtca
    52801 ttctggttgc cgctgacctg acgccgtccg aaaccgcaca gctgaacctg aagaaggtgc
    52861 tgggtttcat caccgacgcg ggtggccgta cttcccacac ctctatcatg gcgcgttctc
    52921 tggaactgcc tgctatcgtg ggtaccggta gcgtcacctc tcaggtgaaa aatgacgact
    52981 atctgattct ggatgccgta aataatcagg tttacgtcaa tccaaccaac gaagttattg
    53041 ataaaatgcg cgctgttcag gagcaagtgg cttctgaaaa agcagagctt gctaaactga
    53101 aagatctgcc agctattacg ctggacggtc accaggtaga agtatgcgct aacattggta
    53161 cggttcgtga cgttgaaggt gcagagcgta acggcgctga aggcgttggt ctgtatcgta
    53221 ctgagttcct gttcatggac cgcgacgcgc tgcccactga agaagaacag tttgctgctt
    53281 acaaagcagt ggctgaagcg tgtggctcgc aggcggttat cgttcgtacc atggacatcg
    53341 gcggcgacaa agagctgcca tacatgaact tcccgaaaga agagaacccg ttcctcggct
    53401 ggcgcgctat ccgtatcgcg atggatcgta aagagatcct gcgcgatcag ctccgcgcta
    53461 tcctgcgtgc ctcggctttc ggtaaattgc gcattatgtt cccgatgatc atctctgttg
    53521 aagaagtgcg tgcactgcgc aaagagatcg aaatctacaa acaggaactg cgcgacgaag
    53581 gtaaagcgtt tgacgagtca attgaaatcg gcgtaatggt ggaaacaccg gctgccgcaa
    53641 caattgcacg tcatttagcc aaagaagttg atttctttag tatcggcacc aatgatttaa
    53701 cgcagtacac tctggcagtt gaccgtggta atgatatgat ttcacacctt taccagccaa
    53761 tgtcaccgtc cgtgctgaac ttgatcaagc aagttattga tgcttctcat gctgaaggca
    53821 aatggactgg catgtgtggt gagcttgctg gcgatgaacg tgctacactt ctgttgctgg
    53881 ggatgggtct ggacgaattc tctatgagcg ccatttctat cccgcgcatt aagaagatta
    53941 tccgtaacac gaacttcgaa gatgcgaagg tgttagcaga gcaggctctt gctcaaccga
    54001 caacggacga gttaatgacg ctggttaaca agttcattga agaaaaaaca atctgctaat
    54061 ccacgagatg cggcccaatt tactgcttag gagaagatca tgggtttgtt cgataaactg
    54121 aaatctctgg tttccgacga caaaaaggat accggaacta ttgagatcat tgctccgctc
    54181 tctggcgaga tcgtcaatat cgaagacgtg ccggatgtcg tttttgcgga aaaaatcgtt
    54241 ggtgatggta ttgctatcaa accaacgggt aacaaaatgg tcgcgcctgt agacggtacc
    54301 attggtaaaa tctttgaaac caatcacgcg ttctctatcg aatctgatag cggcgttgaa
    54361 ctgttcgtcc acttcggtat cgacaccgtt gaactgaaag gcgaaggctt caagcgtatt
    54421 gctgaagaag gtcagcgcgt gaaagttggc gatactgtca ttgaatttga tctgccgctg
    54481 ctggaagaga aagccaagtc taccctgact ccggttgtta tctccaacat ggacgaaatc
    54541 aaagaactga tcaaactgtc cggtagcgta accgtgggtg aaaccccggt tatccgcatc
    54601 aagaagtaat tcttgccgca gtgaaaaatg gcgcccatcg gcgccatttt ttatgcttcc
    54661 gccagcggcg gcaaaatcaa ttcatcgctc tcatgctgct gggtgtagcg catcacttcc
    54721 agtacgcgca accccgcacg gtgcacagca tcgtttaacg ccttcccttt cagcaaacca
    54781 ctgataagct gagcacaaaa caggtcgcca gtccctttca ggtcggtttt tacccgtgaa
    54841 tgggaaatga cattcacgct gtcggcactg accaccacca cctgcatctc ctgattttct
    54901 tcattaccgg aggcgctggt aatcaccacc cattttaatg tgtctgaaag cagacttttt
    54961 gcggcagcaa tggcactgtc gagatcgcgg caatttttac cggtcaggat ttccagctca
    55021 aagatattgg gggtaattcc ctgcgccagc ggcagtaaat attgtcgata cgcctcggga
    55081 aggtcaggtt tgacataaat tccgctatca atatcgccaa tcaccggatc gaccatgatc
    55141 aataggtcag gatggtcttt gcgtagcgca gtcagccact cggcaaggat tttgatttgc
    55201 gatgccgttc ccatatagcc cgtggttaca gcacgaagtt ggcgcagcgc atcacgctcc
    55261 tgaagcgcac gcaaatagcc gctaaaccat tcgtccggaa tcgcaccacc gtagaaagtg
    55321 tcataatgcg gcgtattgct cagcaatacc gtcggcacgg caaagacatt caagccgttc
    55381 tgttttatag caggcaccgc aatgctgttg cctacgcttc cgtacactac ctgcgactgc
    55441 acggcgacga tatccgcctg cagcgcccta ctcttatcgt taaacaacaa caaactactc
    55501 atttaatttt ttctccttgc cgatgatcct catcgtattc caaccgaaac tttacctgat
    55561 tctggcagtc aaatcggcta ttacaaaaca aagataaggt aattcagtga agaaaatcat
    55621 ttgtctggcc attacgctgc taatgacact ccccgcttac gcgaagttaa ctgttcatga
    55681 agaagcccgc atcaacgcca tgctggaggg attagcacag aaaaaggatt tgatatttgt
    55741 gcgcaacggt gatgaacata cctgcgatga agcggtttcc cacctgcgtc tgaagctcgg
    55801 caatacccgt aaccgcattg acactgccga gcagtttatt gataaggtag cttcgtcgtc
    55861 atcgattacc gggaagccgt atatcgtgaa gatccccggt aagagcgatg agaacgcaca
    55921 gcctttttta catgcgttaa ttgcgcagac ggataaaacg gtgcctgcgg aaggaaatta
    55981 atccgctttg gtaaggcatt tacaggaggt aacatgaaaa aatgctttat ttatcacgat
    56041 gaaaaatcga ataaattttg gtggatagat tacgaagggg atagtttagc tgtcaactat
    56101 ggcaaggtag gtagtattgg taaattccat acaaaagaat tcgataatga agaacagtgt
    56161 ctgaaagaag ccagtaaatt gattgccgca aaaatgaaaa aaggatatca agaaactcca
    56221 gactttaact tcatggatct ctactatttt gatgatgaag agattgggtt acatcttaaa
    56281 acgtcacacc caaacttcca gtgccatttt actgatccac tttatatgtg ttgctgggat
    56341 gaagaatctc cttttggtag cgatgaaggt gctgatgctc taaacgaact tgaaaatagc
    56401 ctccgttctg gactgtgctg atttccctca aatgttaatt gaaactatat ggggtatgaa
    56461 atacatcgct atggacagta ttcttgaaga ggatgtgcgt gcgcaattac tagccgatga
    56521 aatgagctct atccagagca atatgattac ctacgcgact gcattcggtc agatcaaagt
    56581 catgggtaaa atctcccata aacttaaaaa gatgggactc aatgcactag cgcgtcatca
    56641 gcttaccgca aaaattcttc aatggggtga cggtcaggac tcaccaatac ttcaaaaaat
    56701 gattgatgac cttactgctt ttccacacga aaattaaaaa ctacatttgt cggcagcaac
    56761 aactgccgac aaatcagatt attgacaaag tgcactttgt ttatgccgga tgcggcgtaa
    56821 acgcctgatc cggcataaaa atattgctaa ttcaatatat tgcagaaaca ttgtaggcct
    56881 gataagcgga gcgcatcagg cagttttgcg tttgtcatca gtctccaatg ctattaatcc
    56941 ttaaatccct gccccctgac taaaatgctc ttccccaaac actccggtgg agaggtagcg
    57001 atcgccacga tcgcagatga tcgctaccac caccgcacca gggttagctt ttgccacccg
    57061 cagtgctcca gcaaccgcgc cgcctgagct gacgccacag aatattcctt cccgcaccgc
    57121 cagttcgcgc atggtgtttt ccgcatcgcg ctgatgaata tccagcacct catccaccag
    57181 cgcagcgttg aaaatccccg gcagatattc cgcaggccag cggcgaatgc ccggaatact
    57241 gctgccttct tccggttgca ggccaacaat ggtcaccggt ttggattgtt cgcgcataaa
    57301 gcgtgaaacg ccggtgatag tgccggtcgt ccccatgctg gagacaaaat gagtgatgcg
    57361 cccgccggtt tgctgccaga tttccggccc ggtggtggtg taatgcgcat aagggttatc
    57421 gggattattg aactgatcga gcagctttcc ttcgccacga ttcgccatct ccagcgccag
    57481 atcgcgcgca ccttccatgc cctgctcttt ggtgacaaga atcagttccg caccataagc
    57541 acgcatcgcc gcacggcgtt cctggctcat gttgtcgggc atcagcaatt tcatgcgata
    57601 gcctttcagc gcggcaatca ttgccagcgc aatgccggtg ttaccactgg tggcttcgat
    57661 taagacatca cccggtttaa tttccccgcg cttttccgcc tcgacgatca tcgaaagtgc
    57721 cgcacgatct ttcaccgaac ctgccgggtt attgccttcc agttttaacc acacttcact
    57781 gccgttatcc ggccccattc gctgcaactt caccagaggc gtattgccta ttgtttgttc
    57841 taatgtactc acgatctcta tccatacgtg gtgttgcctg atgcgacgct tgcgcgtctt
    57901 atcaggccta caggttacaa accttgccat taaaaagccc ggatcgcggg aagcgcctcc
    57961 gggcgtttaa cattcactca acctatcagg cgctttgtgc gagagcaagt tcctcatcgc
    58021 gggtttcaat acgctcgtcg ccgttataca gccgcgcatg ttgcagacca acgaataaac
    58081 gctcgccacg ctgcggggca tcgtcgccat gcatcacgac cgtcagcggt tcgttgtacc
    58141 accccagcgg ctgcaccact aattgggtgt agtgaccttt cgggctggct tccagtacct
    58201 gtaccggcag cggcgaatcg agactggtac ggcggctgat atccacttcc cacgggcgca
    58261 ggaagagatc caccggcccc tgatacgcag gtgtgtagcc cagcggccag cgatgcgcgc
    58321 caacatggaa ctgcccgccg cgaatggttc cctgcaggcg gttcacttcg cccataaatt
    58381 cgagtacaaa acgggtcgct ggttcgcgcc atacctgatt cggcgcatca gcctgttcaa
    58441 tattcccctg actcatcacc actacacgat cggctacttc ggtcgcctct tcctggtcgt
    58501 gggtcacaaa cacgctggtg aatttcagct cttcatgcag ttgacgcagc cagcgacgca
    58561 gctctttacg cacctgcgca tccagcgcgc caaacggttc atcaagcagc agaatttgcg
    58621 gttctacagc cagcgcgcgc gccagcgcca cacgctgttt ctggccgccg gaaagctgcg
    58681 ccggataacg atccgccaga tgcgcaagct ggaccatttc cagcaatttt gtcactttcg
    58741 ctttgatggc tgcggcattc gggcgctcgc gacgcggcag caccgtcagg ccaaaagtga
    58801 tattgtcgaa taccgtcata tggcggaaca gcgcgtaatg ctggaacacg aaaccgactt
    58861 tacgatcgcg cgcgtgcagg cgactcacgt cggtgccgtg gaagcgaata tgcccgctgg
    58921 tttgatgctc cagcccggcg ataatccgca gcagcgtggt tttcccggaa ccggacggcc
    58981 ccagcaacgc gaccatctga ccagaaggaa tatccagtga gatatcgttc agcacctggg
    59041 tgcgaccaaa agacttcttg atattggcaa tctcaatgct catgatgctc ctcctgctgc
    59101 gcgcgttttt cctgattctc caggcgccac tgcaacatac tctttaaaaa cagggtgata
    59161 atcgccatca gcgtcaacag cgcggcggcg gtaaaggagc cgacggtgtt gtagtcctgc
    59221 tccaacaatt caatctgtaa cggcagcgaa agtgtttcgc cgcgaatcga gccggaaacc
    59281 accgacacag caccaaactc gccaatcgcg cgggcgttgg tcagcaccac gccgtaaagc
    59341 agcgcccagc gaatattcgg caatgtgacg cgacggaaca tctgccagcc ggacgcgcca
    59401 agcaaaatcg ccgcttcgtc ttcctggctg ccctggctta acatcaccgg caccagctcg
    59461 cgcaccacaa acggacacgt cacaaagatg gtgaccagca ccattccggg ccaggagaac
    59521 ataatttgca gattatgttc atccagccag ccgccgagcg ggccgttgga gccgtagaac
    59581 agcagataca ccagaccggc aaccaccggc gatacggcaa acggaatgtc cagtagcgtc
    59641 agcagtaact ggcgtcccgg gaagttaaag cgcgtcacca gccaggccag cagaatgccg
    59701 aacaccaggt ttaccggtac ggcaatcagc gcgatcatca ccgtcagcca gatggcgtgc
    59761 agcatgtccg gatcggccag attctgtaaa accggcatca gccccttgct gaatgcctgc
    59821 acgaagatgt aaatcatcgg caccagcagg atgaacgccg aaaccagcat cccgatgcca
    59881 atcagaaacc atttgcccca gttaatcggg cgcgcgtcat aacgcttcaa ttgggtaact
    59941 tccgccatta atgacctacc acacgccgac caaagcgact ttgcagagtg ttaattgaga
    60001 acagcagcag cagagatgcc gcgaggatca ccgaagcaat cgcgctcgct gccgggtaat
    60061 caaactcctg taagcgcaca aaaatcatca gcgacgtcac ttccgtcttc cacgcgatat
    60121 ttccggcgat aaaaatcacc gcgccaaatt caccaagact acgggtaaac gacagcgcca
    60181 cgcccgccac cagcgccgga gaaagctccg gcagcaccac tttgcagaaa ctctgccagc
    60241 gcgttgcacc aagcgtttcc gccgcttctt catattccgg gcctaactct tccagcaccg
    60301 gctgcacggt acgcaccaca aacggaatgc tggtaaaggc catcgccacc gcaataccaa
    60361 gccaggtata ggtgactttg atatcaaact tcgccagcca ttcaccgtaa aaaccgttta
    60421 cggaaaagag cgaggccagc gttaaaccgg cgactgccgt tggcagcgca aagggtaaat
    60481 ccatcagcgc atcaagcagc gtgcggcccg gaaagcgata acgtgttaaa atccacgcca
    60541 tcagcagacc gaaaacgccg ttaaaaatcg atgccacaaa cgccgacagc agcgtcactt
    60601 tgtaggccgc gaccacctgc gggttggtga tcacctccca gtactgcgcc cagctcatct
    60661 cagaaagctg catcaccagc gcagagagcg gcagcagcaa tatcaggcac acaaacagca
    60721 gactggtgcc aaggcttaaa gtaaagcccg gcagcacgcg tcttgaagaa actgcaaaca
    60781 ttacttacgc cccgccgcta acagcttgtc taactcgccg ccgctggtga agtgggtttt
    60841 catcacttcc ggccatgagc caaatttgtc ttccacgcgg aacagttcgg tctgcgggaa
    60901 tttatctttc agtttgtcca tgacttccgg gttattcaca cggtagtaat agtcggtgat
    60961 gatggtctgc gcctgtgggc tgtagagcca gttcaggtag gcttttgcgg ctttttccgt
    61021 accgttggcc tgcacatttt tatcgaccca cgccaccggg aattccgcca gaatattggt
    61081 tttcgggatc accacttcaa agccctgtgc ttcatactgt ttacggatgt tgttcacttc
    61141 cgattcaaag ctaatcagca catcgcccaa gccacgctcg gcaaaagtgg tggttgcgcc
    61201 acgaccgccg gtatcgaaca cttcaacgtt tttcaggaac tgggtcataa actgttcggt
    61261 tttggcttca tcgccaccgt cagctttatc cgctgcgccc catgccgcca gataggtata
    61321 acgcgcgtta cccgacgttt tcgggttcgg gaaaatcagc ttcacgtcgg agcgcaccag
    61381 gtcgttccag tcgtggatat tcttcgggtt gcccttacgc accaggaagc ccatggtgga
    61441 gtagaacggc gagctattat tcggcaggcg cgactgccag tcagccggga tcagcttgcc
    61501 tttgtcgtgc aggatttgta cgtcggtcac ctggttataa gtgacaacgt ctgcttttaa
    61561 gccctgcaaa atcgccagcg cctgttttga tgacccggca tgagattgtt ttatcgtcag
    61621 tttgtcgccg ccgttatctt ttgcccattg ctgttcaaac ggcggattca gggcggcaaa
    61681 cagctcgcgg gagacgtcat aagaactgtt cagcagttcc gttgcctgta catggcccgc
    61741 cagcagcaga gaagcgacca gcgcgagtga gttctttttc agtaagttaa cggccattgc
    61801 gcacccttat aaatttaatg actttctaat agccatcata tttataacgg acgtgaaagg
    61861 agtaacggtt ttatataccg tttggtgatt tggaagttaa aaagggaata agaacttggt
    61921 tcaacaggcg taattgttgc aggcagtttg gacacagaca gcgcggagaa accggagcgt
    61981 acacatagta cgtgaggatt tcgagcactg cccggggcca aaatgacaaa tgaaatagcc
    62041 tggtgaactt agttcaagac gaaatcctcc ccacacatgc agggaggaaa cagaggtgaa
    62101 tcagataccg acgctaaccg tctccggcag tgtgctgccg ccatcaatca cattctgtgt
    62161 accggttaaa tagctggatt catccgatgc gaggaaggcc gccagttcgc cgacttccag
    62221 cggatcggcg aggcgacaca gcgggattgc ttttgccatt tcagtcagca ccgattctgg
    62281 atcttccggg ttcgactggc gggcaatgct ttccgccatt ggcgtgcgga cgtatcccgg
    62341 gcagatggcg ttaacgcgaa taccggattg cgcgtactcc accgccagcg atttagtcag
    62401 gccaacaatc gccgctttcg ttaaggcata cgccgtttcg ccaggatcgg ccaccatatc
    62461 accagtgact gaagacatca tcacaatgcg accatctttg cgcgcaatca tctccggcag
    62521 caccgccttc gtgacgttcc atacgccttt aatattgata tcaatatgga aatcgcgatc
    62581 ttcatcgctc atatcgagga aactgcccag acgacaaacg cctgcgttat tcactaggat
    62641 atcaatgcgc ccttcttttt ccttcgcgcg tttgatagct gcggctaccg acgccgggtc
    62701 acgcacatcg gcgacaaccg ccgtacagcg atgaccacga ccacacagtt cgtccgctag
    62761 cttttcgatc tcaggggaga tatccagcaa gattaggttc gcgccatgac gtgcaaaagt
    62821 tctggcaatt ccttcgccaa ttccctgcaa tgcgcccgta atcagtgctg tcttgcccgt
    62881 gagtttaccc attttaatgc tctccttgtt aatacagagc gttacactcc attccctctg
    62941 aaaatacagg ataaacaaaa tattgctgcg gatcagctca ctaattcagg aaaatctcaa
    63001 tccagtcgct gctttatgca tcatttcagg cgttgtgtta gctcactgct gcgctgaatc
    63061 attttcaatg acgccacatc attgatgttg cacaagacca acaaacagta aatcggtaac
    63121 agaattttgc gccgtgcggg tagacatcga cgagctacgc cattcggttt caccggaaac
    63181 ggtatcgagc gtgaaatgcg ccagccgccg cagcggagag tcagccagag aagtaatggc
    63241 aatcaccgtt gccccctgct ttcgtgccgc ttctgcgcac aagacaatct cttttttact
    63301 gccgctgtaa gagatagcga tttgtacatc gccttttttc aacgcctggg aaacggtggc
    63361 ttgtacgtgc gtgtcggctt cgcaggccac gcgatagcca attttcatca gtttgaacga
    63421 cagatcgcgc cccaccagcg ccgatccgcc caggcccgtt atctggataa acggtgcttt
    63481 actgatgacc tcaatgattt tttgcagccg cgcgtagtcg aacaacgcac aggtttgctc
    63541 cagcgccagc tctttttcgc gattcagttt gcgggcaatc acttccagcg aatcatcact
    63601 ggtaatcgaa ctgtgcaggt gcagcgccgt ggcattcgtt ttctcccggc tggcactgta
    63661 ttcgccaatt aacgccatac gcaattcagt aaatccttgc gcgccgagct tctgggcaaa
    63721 tttcacaatg ctcgactggc tgataccgag ctgtttcgcc atctgccgtg aagagacgga
    63781 tttcagctcg ctcactctgg cccgcagaaa gtcagcaatt ttttgctcgt tcccggtaaa
    63841 ttcactttcc gcattgcgta ttttcgtcag gtacaacatc ttccactctc ctgggctggc
    63901 agcaaaaatt ccgcttaagt tcttatgacg ctcttcacac tctgcgagtg tagactcaat
    63961 gattccttta agtctgtcaa ttcgggatat atgattccat acatctgcaa ttatttgatc
    64021 gtaaatagta aggtcaccac cgatgcaact agaaaagatg attactgaag gctcgaacac
    64081 cgcctcggct gaaattgacc gcgtatcgac gctggaaatg tgccggatta tcaacgatga
    64141 agataaaacc gtaccgcttg ccgttgatcg cgtcctgccg gatatcgccg cggcgatcga
    64201 tgttatccac gcccaggtca gcggcggcgg tcgtctgatt tatctcggtg cgggaacatc
    64261 cggtcgtctg gggattctgg atgccagcga atgtccgccc acctacggcg tgaaaccggg
    64321 tctggtggtt ggtttgattg ctggaggcga atatgccatt cagcacgcgg tggaaggcgc
    64381 tgaagatagc cgggaaggtg gtgttaacga tctgaaaaat attaatttaa cggcacagga
    64441 tgtggtggtt ggcattgctg ccagcggtcg cacgccgtat gtgattgccg gactggaata
    64501 cgcacgccag ctcggctgcc gcacagtggg aatttcctgt aatccgggga gcgccgtttc
    64561 aaccaccgct gagtttgcca ttacaccgat tgtaggtgcc gaagttgtta ccggttcttc
    64621 gcggatgaaa gcaggcacag cgcagaaact ggtgctcaat atgctttcca ccgggctgat
    64681 gattaaatcc ggcaaagtat tcggcaacct gatggtcgat gtggtcgcca ccaacgaaaa
    64741 actgcatgtg cgccaggtca atatcgttaa aaacgccacc ggatgtaatg ccgaacaagc
    64801 ggaagcggcg ttaattgcct gcgagcgcaa ctgtaaaacg gccattgtga tggtgctgaa
    64861 aaatctcgat gccgcagaag ctaaaaaacg cctggatcaa cacggcggct ttattcgtca
    64921 ggttttagac aaggaataac ccatggccaa agagatcagc agtgaacttc tgaacaccat
    64981 tcttacccgt gtcggcggac cgggaaatat cgccagttgt ggtaactgta tgacgcgcct
    65041 gcgtctgggt gtacatgaca gttcactggt tgatcccaat atcaaaacgc tggaaggcgt
    65101 gaagggcgtc attttgacca gcgaccaggt gcaggtcgtt tttgggcctg gtaaagcgca
    65161 tcgtgccgca aaagcgatga gcgagctgct gggcgaggca ccggtacagg atgccgcaga
    65221 aatcgccgcc cagaacaaac gtcagttaaa agccaaacaa acctccggcg tgcaacaatt
    65281 tctcgccaaa ttcgccacca tcttcacgcc gctgatcccc ggttttattg ccgccggtct
    65341 gctgctgggg atagcgacgt taattgccac ggtgatgcac gttccggcag atgctcaggg
    65401 cacgctaccc gacgcgctga attttatgaa ggtgttcagc aaaggtttgt tcaccttcct
    65461 agtgattctg gtgggctata acgccgccca ggcatttggc ggcacgggcg tcaatggcgc
    65521 aattattgcc gcactctttt tgctcggtta taaccccgct gcaaccaccg gttactacgc
    65581 cggttttcac gatttctttg gtctgcccat cgatccgcgc ggcaatatta tcggcgtgct
    65641 gattgccgcg agggcctgcg cccgcattga aggcatggtg cgccgcttta tgccggacga
    65701 tctcgacatg ctgctgacct cgttaatcac cctgctgatc accgctacgc ttgcgtacct
    65761 gatcattatg ccgctgggtg gctggttatt cgaaggtatg tcatggctgt ttatgcacct
    65821 gaacagtaat ccgttcggtt gtgcggtttt agccgggctg ttcctgatcg ccgtggtatt
    65881 tggcgtgcat cagggcttta ttcctgttta cctcgcgtta atggacagcc agggattcaa
    65941 cagcttattt cccatccttt caatggcagg cgcgggccag gtgggcgcgg cgctggcact
    66001 ctactggcgg gcgcaaccgc acagtgcgct acgcagtcag gtacgcgggg cgattattcc
    66061 cggcctgctg ggcgttggtg aaccgctgat ttacggtgtc accctgcccc gcatgaagcc
    66121 gtttgttacc gcctgtttag gcggcgcggc gggcggtttg tttatcggat tgatagcctg
    66181 gtggggtctg ccgatgggct taaacagcgc ctttgggccg tctggtctgg tggcgctgcc
    66241 gctgatgact tccgcacaag gcatccttcc ggcaatggcg gtttatgctg gcgggattct
    66301 ggtggcatgg gtttgcgggt ttattttcac cacgctcttt ggctgccgta acgtcaatct
    66361 ggactgatat tatgaaacgg acaatgctct atctttctct gctggctgtt agttgtagcg
    66421 tcagcgccgc aaaataccct gttctgacag aaggtagttg atcaagtata tgcggcgtta
    66481 aagcagtaat aaaaaggcca gtcagtaatg ttactgcctg gcctttaatc aatgaatcag
    66541 aaacgcttac agcaccatca acttgtccag cgacggtgcg aaataatagc cgccggttac
    66601 cggtttggtg aaacgcaaca tcgcatcacg tttgccatcg gtatcgccaa acatgctcag
    66661 cagttgctgc tcaatgttat gcagacgcgc gcagtaggcg cagaagtaca gaccgtgagt
    66721 gccactggcg gtgccgtacg gcaggctctg gcgaacaatc ttcagccctt tgccatcttc
    66781 tttcagatca acgcgggtga ggtgagaggt ttccggacgt tcgtcgccgt caatctcttc
    66841 gttggcctct ttggtgcgcc cgatcatcat ctcctgatcg tgaacgctca tccggttgag
    66901 ctgcttcagg ttgtgctccc aacgctgcac aaacacatag ctgccgccgg catccacgcc
    66961 gtctttgata accgccactt cgcggcgcgt ctcttcaccc gccgggtttt ctgtaccgtc
    67021 aacaaagccg ctcaggtcac gctcttccac ccaacggaag ccgtggatct cttctttcac
    67081 ttcaatgcag tcaccaaagg cttccatcgc cgcctgagcg acagagaagt ttacgtcgtg
    67141 acgcagagaa agaatgtgga tcaacacatc gaactgggtc gtcggcgcaa ggcctttacc
    67201 gtagcccgga aaatctttca gctcttctgc cccaacgccg ccgctcagag cgcgccaggt
    67261 gttgttacca aaggcaacca ccgcaccaag atgcgcgtcc gggaatttcg cttcaaaagt
    67321 tgccagtttg tcggcaaatg ttttactggc cgcacgcagg gcgtcaactt cccctttcac
    67381 gttggcttcg atccaaatcg ccgcgcggca atgttctggc aaaatgccac tctgaacctg
    67441 agacattgtt cctcctgaaa ataagaatgc cacgcaatcc gtggcattgt tgagcgctat
    67501 tttacgtgtt ttttcgcgac ggcggtttgt tcagacgcaa attaacgccg ccagataatt
    67561 ttactgactt tccagttttt cagggtatcg tccgaaggca ttaacccttc aggaccacgc
    67621 cattccccgc tgaactggta gctaatatgt tgactgcctt cggctttaca ttcgacggca
    67681 cgattatcat caccatcagc tttttggcaa ttgccaaatg ctttgctgta aaggtcgcta
    67741 aacggtgtac cgattttaac accggtgtca gcaggaatat cgctatccag cacgtcaatg
    67801 cgactgatcg tgccttgatc gccgttaatc accatcgcca cgttgtcgcc tttcatcact
    67861 tcaaaaaagc gcaccacgtt gccgttcgcg gttttcattc cgctgcgcag gcgataatcg
    67921 ccatcaagcg catcagcaat ggcttgttct tgcagtggtg tggacgccgt taattcaccc
    67981 acgccctgct cgctcacttt ggtggacgat ccaaaccagt tccacgggtt agcggcagac
    68041 cagttaactg acgacatcgt ggaacagccg gtcagcatca acggcatagc gcataacatt
    68101 aaacgcagcg atttcatgtc acttcctttg gttattcaat aacgttgctt ggagtgcaaa
    68161 ttcaccaaaa agtgccgtta ttctttttct tcattaaaac aagcgcgtaa acgtcgattg
    68221 gtcagcagcc agattaacgc cacaatatcc gccactacca gcgccagacc gataccgcta
    68281 acggattcac cgttcagcca cagccacggt tgccagcaaa gtaaaaccac ctgcgccaac
    68341 agcaacagaa aatagagcac atgccaggtg cgaggaaacg aagcccgccg accgctcaac
    68401 aaaaacgcca gcaccgccgg aataccggga atcagcccca gccagaaatt atcgtgatcg
    68461 ggataaaaca gatttagcag cgcagtaccc tgctcgcgcg acgcaccggc aatgacaaac
    68521 agcacccagg ttcgcgcctg aagcaatagc acaagccaga agagcaaggg taaacgcagg
    68581 cgtccgtgcg catcataatg gacaggatgg aactcagtac tcttcatctt caatcaaacg
    68641 cttacccaga ctcagcacgt cggcgtgttc atatcccagg cgttcataca ttccgagcac
    68701 catgtcgtta tcttccggca cgttgatctg aattttcggg cagccgcgag caatcagctt
    68761 tttctccagc cggttaagca acgcattggc aattccacgc ccacgaaact ctggatgcac
    68821 gccaagataa taagcagacc cgcgatgccc gtcataaccg cccatcaccg ttccgaccac
    68881 ttcaccgttt acctcagcga ccaaaaacaa actgacgtca tggttcatct tacgctcgat
    68941 gtccatttcc ggatcgttcc acggactcag caagtcgcaa cgctcccaaa gggtgatgac
    69001 ctcttcgaaa tcttcctggc gaaatacgcg tatctccatg gtattcgtta cctttttgcg
    69061 ggttaaaagg ctgattatgg cgtgaacggt cgaattagcc aatatctgac gaaaatcggt
    69121 tgaaaaagtg gcataatggg gagttgtcaa ctattgaaat gaaaagtaaa acaattctca
    69181 acagcaaacc gtcgtaacgg attacgcgat acgatataac atctggaact ttattattac
    69241 aactcaggcc gtatgagcac ttttaaacca ctaaaaacac tcacttcgcg ccgccaggtg
    69301 ctgaaagccg gtttagctgc cctgacgttg tcaggaatgt cgcaagccat tgccaaagac
    69361 gaacctttaa aaaccagcaa cggacacagc aagccgaaag ccaaaaaatc tggcggcaaa
    69421 cgtgtcgttg ttctcgatcc aggccacggc ggaattgata ccggagcgat cggacgcaac
    69481 ggttcgaaag aaaaacatgt ggtgctggcg attgctaaaa acgtccgttc cattttgcgt
    69541 aatcatggga ttgatgcgcg tttaacgcgt tctggcgata cgtttatccc actttacgat
    69601 cgcgttgaaa tcgcccataa acatggcgca gatctgttta tgtcaattca tgccgatggc
    69661 tttaccaacc cgaaagctgc cggtgcttcg gtatttgccc tctctaaccg tggggcaagt
    69721 agcgcaatgg cgaaatacct gtctgaacgc gaaaaccgcg ccgatgaagt tgccggtaaa
    69781 aaggcgactg acaaggatca cctattgcag caagtactgt ttgatctggt gcaaaccgat
    69841 actattaaaa acagcctgac gcttggctcg catattctga aaaagattaa gccggtgcat
    69901 aaactgcaca gccgcaacac cgaacaagcc gcgtttgtgg tgttgaaatc gccgtcggtt
    69961 ccttcggtgc tggtggaaac ctcgtttatc accaacccgg aagaagagcg gctgttaggc
    70021 acggcggcgt ttcgtcagaa aatcgccaca gcgattgctg aaggcgtgat cagttatttc
    70081 cactggttcg acaaccagaa agcacattcg aaaaagcgat aagttatgaa acccgacgca
    70141 caccaggtta aacagtttct gctcaacctt caggatacga tttgtcagca gctgaccgcc
    70201 gtcgatggcg cagaatttgt cgaagatagt tggcagcgcg aagctggcgg cggcgggcgc
    70261 agtcgggtgt tgcgtaatgg tggtgttttc gaacaggcgg gcgtcaactt ttctcacgtc
    70321 cacggtgagg cgatgcctgc ttccgccacc gctcatcgcc cggaacttgc cgggcgcagt
    70381 ttcgaggcga tgggcgtttc actggtagtg catccacata acccgtatgt tcccaccagc
    70441 cacgcgaatg tgcgtttttt tattgccgaa aaaccgggtg cagagcccgt ttggtggttt
    70501 ggcggcggct tcgatttaac ccctttctat ggttttgaag aagacgccat tcactggcac
    70561 cgcaccgccc gtgacctgtg ccagccattt ggtgaagacg tttatccccg ttacaaaaag
    70621 tggtgcgacg attacttcta cctcaaacat cgcaacgaac agcgcggcat tggcgggctg
    70681 ttctttgatg atctgaacac gccagatttc gaccactgtt ttgcctttat gcaggcggta
    70741 ggcaaaggct acaccgacgc ttatttacca attgtagagc gacgtaaagc gatggcctac
    70801 ggcgagcgcg agcgcaattt tcagctctac cgtcgcggtc gttatgtcga gttcaatctg
    70861 gtctgggatc gcggcacgct gtttggcctg caaactggcg ggcgcaccga gtcgatcctg
    70921 atgtcaatgc cgccactggt acgctgggag tatgattatc agccaaaaga tggcagccca
    70981 gaagcggcgt taagtgagtt tattaaggtc agggattggg tgtaactccc tcacccccat
    71041 tcccgcatcc gctgatgcaa cgtcaacgac ggcttctcgg caaacagctg ctggtaatcc
    71101 gtggcaaatt gccccagatg ccagaatccc cactgcatgg cggcgtcttt taccgttgtg
    71161 ctttgcgacc acgggcttat cagttcgcgg cgtacggcgt tcaggcgaat gcgtttcagc
    71221 cacgcgtttg ggccaatgcc taaaatagcg tgaaacgcgt tttgtagcgt gcggcgactg
    71281 acatgcagtt gattacacaa atccagcacc gtcaccggtt cggacatgtt ttccagcaca
    71341 tattcacggg cgcgggaaag caaccgacgg taactctgat gactgatgct ttccgccgtc
    71401 accattggtt gcgcttcttc cagcatcgcc cccatcgcca ttagcaaatt atcccccagc
    71461 acttttcgca ctgcaggctg atggagattt tccgggttct cgcaaaacgt cgccagcgcc
    71521 tgttggacaa agccccacag cgcggcttta tgctgcttct ttcacttcca gcgccgactg
    71581 gctacgcagc atatgtaata cccgatccgg attgtgcaaa aagttagcct gacaggtgat
    71641 gacatcttca gacagcacta cgcccaggat cgtataatcg tccggcgtac ttaattcaaa
    71701 ctcggttcca ccggggcgcg ttgctatttc tgcgcttccc agacattgcg aaccgataaa
    71761 tccctgctca ccgcgcgtcg ccggaatgcc aaaccagaac gagttcggcc agaccaggca
    71821 cgactgacgc agcgccagac cggtgtattc acgaaaaacc tgaatatcat cgagtagaat
    71881 ttccgtaaat tcaccatgaa acttgcccgg atgcagctga tcgtaaatct gctgccaggc
    71941 ggtaatcgtc aaagcatgtt catagacatc cgttgtctgt cgttgatgaa cattatccac
    72001 ttcgaccttc ggcgtgagct tcaggttttc gggtaagggt tcatgataaa gatggtgcaa
    72061 attggctgta cgggtctttt tcatgatgtt aatgccgggt gttgtaggac acccggcacc
    72121 tccgacaggt taatggggct tgagacgata acgactactg cgtttacgtt acgtcccggc
    72181 agaaaagagc tgttcgagcg catttctggc tttttccagc ggccagccaa agtgggcggc
    72241 cacttctcct gccgtcattc cctgacatac tgatgttaac agcgccaaca actcgtcagc
    72301 agattccgcg acgataactg gcgcgtcggg ttcctttacg ggttgcttcg gctggcggtt
    72361 aaagccagta accagccact gggtgtcatc gtctggccga ccgatctcct tacggctgat
    72421 gacacgcccg gtacgcatcg cggcagcaca acctgcgtcc agcgccgcac gacacgccgc
    72481 cagatcgcct tccaccacca gcgttaagcg accagggtca agcacttcgt gactgagcag
    72541 acgaacgtta gctgctttaa gcatggcatc cgctgcatct atcgcggcga ccatgccgtc
    72601 cacttccagc aatcccagtg cattgatcat cggcaacctc cgttacgcac gctggattgg
    72661 attacgcgcg atttccagca ctgcatcggt aaaggcgtta caggctgctt tacacgcggc
    72721 ctggctaccg gttaaaaatg ctgccgagta gttggtttca gacggtggcg ggacgtaggt
    72781 caccagctgt acgtcggcag atttcaacgc ggcatcaatg ccgtaggtcg cttccagcgg
    72841 cggtgccacc agatacgcca tcggatcgcc aagcgtgatc ccggcggttg atgaaagata
    72901 agaacctgta cgcgaaacca catgcgccag gaaggcggtg ttttctgcgt cattggccca
    72961 ctggaaagct gcgccatttt caatatgtgc aaccatcgca tccagaccgg cacgcacttc
    73021 cgccgggttt ggcccaccaa gcataatcag cacttcaccg gcagtcggtg acgggccgtg
    73081 tgccgctccc gcatacagcg agcgaccata taccacttcg accatcgctt gtttggtcgc
    73141 ttcgtcagcc gcaatgtagg ttacgtcgtc agaatcggca gaaatcagcc cgaggctacg
    73201 aatatgcggc ggcaatttta gttcacgcgc aaattcagcg ttaacggagg caatcacccg
    73261 catggcggtt accgacggtc gaatcaaatc taaagctggc atgatgcctc cttatcgggt
    73321 catgttgatg ccggatgctt tctgctccag catacgtttc gccaaatcca caatgacggc
    73381 tgcggcttca accggcggcg tgccgccctg gtgaatgtta gagatacagg tgcgatcggc
    73441 ctcaacggtg gtcgccatac gcggcgagta aacggcgtag caggagaggc tttctgactg
    73501 ccctaacccc ggacgttccc ccaccagcag gatcaccact ttcgcgccga ggatctcacc
    73561 gatctgatct tcaatcttca cgcgaccata acgaacaaag aatggcgtgc cgactttcag
    73621 cccggcctgt ttcaggcccg ccatcagcgg cggcaggatc tcttcgtagt tgacggtgat
    73681 ggcatcagtt gacaagccat cagaaataac gacctgtacg tccggattag cagcaacaca
    73741 ctgtgctttc agcgcttcaa cagcttctgc acacaggcgg cggcccatat ccgggcgagt
    73801 caggtagaga tttttgtcgc taatttcaga gcgtacttcc agcaagccct gcgctttcac
    73861 ccactcttcc ggcacttctt tcagtacggt atctttcgaa cgagagtgat cggccaagaa
    73921 acgcagcagc gcctgagtac gtggacgcgg accggcacga ccggtacaaa cgcgggccac
    73981 ggtgctgcgg cgcagttctg ttaatacgtc tgcgcgatgc gggttttcaa caccaatcca
    74041 cgcttttgct tcagcggaac ctaaatccag cgcacagctt tccgaggtca ccggtgccgc
    74101 acagttggtg gtggcgcact ttgcttctga cggcgcgggg gccgcttgtc ccattgacgc
    74161 catcacgctg cgtacaattt cttcaatctg tttttgatcc atgatgtgtt atctccgcgt
    74221 catcagaaga acagtgacgg atcgcccgcc cgtttggtca ggcgaccgtt tgccataatg
    74281 cccatgcttt ccagccagcg ttcaaactct ggtgacgggc gcaggttgag taactgacgc
    74341 acagtggcgg tatcgtggaa tgcggtggtc tggtagttga gcatgatgtc atcacccagc
    74401 ggcatcccca tgatgtagtt gcagcctgcg gtggcgagca ggatcatcag gttttcgttg
    74461 aggttctggt cagagtcagc gtggttggtg tagcagccgt cacagcccat agagatgccg
    74521 ctcagcttgc ccataaagtg atcttctaag ccagcacgga taatctggcg gtcgttgtag
    74581 agatactccg gcccaataaa gccgaccacg gtgttgacga taaacggatc gtaatgacgc
    74641 gccagcccat agttacgtgc ttccatcgtc acctggtcag cgccgaagtt agcgccagcg
    74701 gatagcgcag agccttgtcc ggtttcgaag tagaggcagt tttccccggc gatacgattg
    74761 aactctgcgc ccactgcgcg cgcttcgtcg agcatcgcca gttccacgcc aaactctttc
    74821 agcccttttt cgctgccaca gatactctgg aaaatcagtc cgccaggagc gccgcgacga
    74881 atcgcttcga tctgggtggt gacgtgcgcc agtacgcagc cctgagttgg gatgttgaat
    74941 ttgtcgatca cgccataaat ggtatccagc acgcggctta agttttccac gtcgtcagtt
    75001 accgggttaa cgccgatcac cgcatcgccc accccgaagg aaagcccttc atagatttgc
    75061 gcagcgatac tttgcacgtc gtcacgggta tcgttcggct gcaaacgggc gctaaaggtg
    75121 cccggaatac cgatggtggt attggccttt ttgattaccg gcattttctt cgcgccgtag
    75181 atcaggtccg cgttggagca aatcttcgct atcgccgcga ccacttccga ggtcagccct
    75241 ttgcgggtaa aggcaatgtc gtccacgctg gtttcatcgc tcagcacata ctcacgcagt
    75301 tcgctgatgc tccagttttt aatctggttg taggccgttt cgttaacgtc gtcctgaatc
    75361 agccgcgtca cgcagtcatc ttcataggca atcaccggat tattgcggat gtccgctacg
    75421 gtcatttccg acaacacctg ctttgccgcc acgcgctcct gtgagcttgc cgctgcaacg
    75481 cccgccagca catcccccga acgcagttcg ttggctttag ccagcacctc ttttacatcc
    75541 ttaaactgat atacattgcc gaacaatgtg gtctttagtt tcataagtcg ttccctcagg
    75601 aagaagtgat gagtaacatt tctcacagcc tgcaaacaat tctatttcat cgttagccct
    75661 tgtcatgtaa ggtgatttcc ttaacacgcc cgttacttat cctgccgaac gcaaccgaac
    75721 tcgccggata catgagcggg gttatcgagt cgttagatcg aggtatccgg ttttgagcaa
    75781 aacaccactc acagcaaagg ccatagacgc cgcacaacca aaggacaagc aatacaaact
    75841 caccgcttca ctgacgcccg gcctgttttt gctggtgcat cccaacggca gcaaatactg
    75901 gcgattccgg tactggatag acaagaaaga gcggttacag gctattgggg tatatccgct
    75961 gattagcctc aaggaagccc gcaaacgcgc cacagagagc cgtttactga tagcccaggg
    76021 aattgaccca atggatttgg cacgcaagga gaaagccatt gatgcactca acatggcggc
    76081 aggttttaag acggtagcgg aggactggct ggctaccagg gtaggcggct ggagtgagag
    76141 ctatacaaag caggtcaaat cagcactgga gaaagacgtt tatccggtac ttggcaagcg
    76201 ttcaatcgtt gatataaccg cccgtgacgt gctggcatta ctccagaaga aagaacgtac
    76261 cgcaccggag caggcccgca agctacgcca gcgcatcggt gagatcttca agtttgccgt
    76321 tatcaccgaa ctggtaacga ggaatccggt tgcagatctt gatacggcac tgaaggcccg
    76381 ccgacctggt cacaacgcgt ggataccgat tagtgaaatt ccggccttct acaaagccct
    76441 tgagagggcc gggagcgtcc agattcaggc ggcaatacgt ttgcttatat taacggcatt
    76501 aaggaccgca gaactgcgtt tgtgccgttg ggaatggata aacctggagg acgcaacaat
    76561 cacattgccc gctgaagtca tgaaggcccg ccgcccgcat gtggtcccgc tatcacggca
    76621 ggcggtcgag ctattgcagg accagttcac acgcagcgga tacagtgttt tcgtgttccc
    76681 gggccgattc atggataagc cattatcagc cagcgcgata ctcaaggcgc ttgagcgtat
    76741 cggatacaag tcaatagcca caggccacgg ctggcgcaca acgttcagca ccgcacttaa
    76801 cgaaagcggc aggtataacc ctgattggat tgagatccaa ctagcccacg tcccgaaggg
    76861 aattagggga gtttacaacc aagcggccta tctcaaaatg cgccgtcaaa tgcttcagga
    76921 ctacgctcat tgccgctcgg ctaggtcaaa tcctcacgat aaagctgttc ggattgtgac
    76981 cataatgtta aaggactctt taaatcattc cttgacgaat taactagctg gcttaagcgg
    77041 aatagcatgc aggataaaaa caaaaccagc ttagcagtgg ggagagcttc gcttcgctcg
    77101 tctcctgggg ccaatgctca atgaaacgac gtaaggcacg gagcgctcac cataggtaga
    77161 cacataaaca tctgagagcg caccttctat cgcgctacgc gctcaataaa aaccaacgac
    77221 accgccacac tgcaagacca atggttttta tcgcgtatac agaccagaca ggaaacagca
    77281 gcaaagcagc gtatagccag gtgcagacag cacactcaca cgacgcacag caggcttaag
    77341 tgatcactca ggaaacttta tccccgccgc cgatcaccgc gaactatcat gaccgcgcca
    77401 aattcgaaag gaatggccca tagaacgcga tgaacacaca taagcgctaa cttgcttacc
    77461 tgaaaactta acgtcgccac agagcgattg agagcgctta aaatcgatgc gatttcatta
    77521 aatttttctc actcgcctga atggtggcga tcaggactga tcgcaacatt gccccgcctc
    77581 gatattgagc gcataatata cacatacatt aagcggaaaa gagataagtt tattgtgaaa
    77641 atcagggggt tacacatcat gcgcatatca gaacaacaac ggcagatcct cgactatctg
    77701 gcaaaaaacg aatcaatcac cgcacgacaa gccgcagatc tcgtttatgg tggcaatgtt
    77761 acccgcgtcc aggttgacgc cgcccgccgc tcactcatga cgatggttag taatgggcta
    77821 atcaggaaac aaggccgcgc tttcgtagct gaaagacata tcaatcacgc ttatagccag
    77881 gacgttgaag aattgcgccg ccagatcctt tgggtattca gcaaacaggg tcaggagtat
    77941 ttcctttctg aacacccaaa tttcgccgcc aaagagttaa cagtaagcgg attgtcgtgc
    78001 gcactggatt atcttgtcgg tgaaggttgc gttacttaca agcagctatg gcacgccctg
    78061 aatacgcttg tagaagatgg tttactcatt gccacgcgtg agaacatcga atactggata
    78121 atgccgcgtt caggtcgccg caatcatgca cggtatattt caggcaacag atacagaacg
    78181 ccggacttta atccgacaca ggaagatcgg gaccgtgccg aaatgatgtg cagggaatag
    78241 gagaaaagaa ttatggataa gttattttcg atgcagtgaa cacaagcggg cgcaatgccc
    78301 gtttttattg ccttattcca cgcctgacga atcaatttga tcacacctga gcaaatgcat
    78361 agccagaagt taaggccgtt atattgcgat tgggagcgtt ttaaacggta tcaatttaat
    78421 taattctgat gtggtgattt gcaggcaaaa aaaacccgcc ggagaagcgg gaaatagaga
    78481 catatcgttg tagttattaa cagatcactt tgaggtcgag ggcggctaac cctcgctaat
    78541 attatcccgc cattaccttg cgggtgcaaa taatctttta ttgctttgat gatttaataa
    78601 atagcacttt ttgctaacaa aaaccgctgt caatatatgg ccaaaaatgt caacagaaaa
    78661 aataaggttt ataatattaa gtatcacatg ggtaatgggt tcagggaatc ggaatccacc
    78721 gcctcacgga aattcaaaat ttgcttcttc tttcctccgt ggggcggctt ctttttcgta
    78781 tcgaggtatt tatgaaagca ataacattag aacagttcgc agcaatggcg aatgtatcga
    78841 ccagaacgct gaggcgctgg ctaaaaaaac catcaaccgg agtccctttg ccatttaccc
    78901 cacgcggtac tcgccaatta ttctgggagc acgaatgcaa agaatggttg tacagaacag
    78961 aacgataata atcacgcatc acattttgag gtaaacatca tgaacaatat cgaaagatta
    79021 gaacgctgtg ccgatattat gcggcgcaga tggatttatg accctgaagc gggtttattg
    79081 tttagccgtg aaacaaaaga cgttatcaaa ggcagcatga ccaataaagg gcatttggtt
    79141 gtaacggtac atgaaggtaa attcaaagtg acactcacct atcagaaagc ctgctatgta
    79201 tacacatacg gcgcttatga cgagacatta tatgaagtgc accacgttaa tttggacaaa
    79261 caggataacc gcatcaagaa tattcgcctg atgccgaaag aaaagtaccg ccggatacat
    79321 aaaaacgtcc gcaaacttat tcggctgggc cacacacaat ggcttcaacc gcaattaatt
    79381 gaacaatcac tataaacaca gtagtaaata ccatgcattc attagacagt tattttcagc
    79441 gcacgacagc ccctaaatca gccgcccagg aacgccgtga ggaattccat gagaaagtga
    79501 tgcgttctgc tgattatatt gcggacaagt tcgtggaaac ggttcgtcca cttgttgatg
    79561 aagtagctga taaacttcag tctgaaatgc cggaagatat ggaaggcact gcaaagcggc
    79621 gactcatttg cgaattaagt cgtcgttttg gcgtgtcaat aagcgctttt aaataatcga
    79681 aaaaaggggg gaaagataat ggacgcattt gaggtactgg caaataaagg cgctgaactg
    79741 gtagcacagc gtgataaaac cgccaacgaa ggggaacgcg cactgctaaa taaacagatt
    79801 aaagcgatcc gtatggccca gtttaaatta atcagtaatg aagttatcga aacaattaaa
    79861 ccgcagatcc cacaaatcgt tgctgacgct attaaagccg ccggactgga gaaacgcatt
    79921 gccgggctaa aaacgaacgc agagaaaatg gagatgcaca aacagagcgg tgctccgaat
    79981 cccgatgagt acttctcacc ggatgttgag tttatggcgc aggttgagga acgtctgggt
    80041 agctgtctca ccgaggaaca gcgtcgctat tttgatggag tggatagttc ggcaggtatt
    80101 gacctgaact cgtatttcgg tcgtgaaatt gagcattttg atgccgcccc gtctatgccg
    80161 gagccagaac cagaaatggc caccgatcct gaagctgagc aacggaaacg cgtattcaat
    80221 gcgataggga ttcggtactg atcggataaa agaaagccgg gaattacccc ggctgactta
    80281 acaatgtgac caatgaacaa atcggacaag acaaacagga taagcagtct cgaaacaatg
    80341 ttcgggaact atattagcac aagtccggag aaaatcatca tgttggcact acgcatcatg
    80401 cagggcatcg ctaagaccct cgcagaacat gtcttagacc tgaaacattc gccactgagc
    80461 aaacaggcca tgaagcgcta aacgcttcgt ctgtgggctg aatattcgct tggaactatc
    80521 aataaaatca tcgacatgaa atcggggccg agcaaccaga gcgcggagga aatggagttc
    80581 atcaggcgtt taattctcat tcgcagagac attcatagcc agttacacag cgtgggaatc
    80641 gatattaatg atgggacggg cgattaattt tcggacaagt actatgcgcg cgcgaggagc
    80701 tgaacatgaa gcaagatatt gcagacaggc tggagatact cgaaggccag cgggcagaag
    80761 cgaaacaact acgtaagcag gccagaagag cgcacaggaa caacgaagca gagttactaa
    80821 ccaaatacat cagtttcacc aattactgca tctacgagtg ttacaaggaa gatgcagagg
    80881 actggctgga ttcattgcca gaacagtact gagcagaata acaccacgtc atgttgttct
    80941 gaggtgagca tatcacctct gttcaggtgg ccaaatttag tgtgccactt cagcatcacc
    81001 gttgaaatca caataacgat ttttttgtaa atatgctctc acatatgtag gaatagacca
    81061 ggttggcatt gaaacacatc taccatgacc ataataaagt aattttttga tatgtttatg
    81121 gttttatatt aattaaaata tatctaaaat tgatagtttt aaaattgcaa aatccagatt
    81181 gtgtttgtaa tgtttaatct gattgtattg agtggcgttt attatgaaaa ataaatcagg
    81241 taattcttgt tatagagaag cagtcgatat tcatagctca cttgagcttt catttatatt
    81301 gcataaatat catttgcgtg tagatgagaa gaaatctcga aatgtattca gcgccatacg
    81361 tgatgagctc atccttgatg gggattcacg acaaaactta gctactttct gtcaaacatg
    81421 gcttgatgat gaaatccgtg agttgcttaa tttagctatt gataaaaaca tgatttataa
    81481 agatgaatat ccacaaatgg cggaacctga aggtcgctgt gtgcgtatgc tttccgattc
    81541 gtggagtttc cccgattcgc gacacacctt aggttgctca accattggtt catctgaagc
    81601 tgctatgctc ggccgcttgg cgttaaaatg gcagtggtgt aaaaaacgcg aagtacaagg
    81661 taaatcaacc gaacctgatt tgcgatcctg tgcaaatatg ctggcataaa ttctgccgtt
    81721 actgtgatgt gaaattgtgt gaaattccac ttgatgatca tcacctgata atgaatgcta
    81781 aagaggtttt gaagcatgtc gatgaaaaca ctattggtgt cgttacgact ttaggtatta
    81841 cttatttttg taaagcagat tgcgttagtg tttttaagat aagtgttaaa gttaatatta
    81901 gtttttatat ttacatcgta attatatgtt tctattttaa aaatattcga gagtataaaa
    81961 atgtctaatg ctaatatcgc acccaaaagt aagaaaatga cagtggggac actcgctatc
    82021 atgaatataa ctgctgtagt aagtctacga tttatacctt ctgaagcaga atacggctta
    82081 ggtgccattt tttactatac ctttgctgca attatgtttt tagtacctgt tgcctttgtt
    82141 gcggctgagc tagctacaac atatccacaa aaaggggggg catttcgatg gataggagaa
    82201 gcatttgggg ctcgctgggg ttttgtagga atgcttatga cctggctaca agtcatcccc
    82261 tactttccaa cagtactgac atttggtgca gtctcggttg catttattga tgttgatatg
    82321 ggggtggcag aatcaattgc cagtaataaa tggtatattt tatttttcgt actatttgtt
    82381 tactggggag ctatttgtgt tgcattacgt ggcgttggta ttttctcgca ggtatctaag
    82441 tggtgcggta ttatagggac aattattcca gctattatcc ttgttattct tggtttctcc
    82501 tatctgtggt tcagcggtaa accagcggtg atcccattag gttggggtga tcttttccct
    82561 aatatagcta acttccagaa tattgttctt gccgccagca tatttcttgc ttatgcaggg
    82621 atggaaatga atgcggtcca tgttaacgat ttagataatc caactaaaaa atacccaatt
    82681 gcaattacca tcgcttctat aggtactatt gcaatattcc tgctcagtac tttaggggtc
    82741 gcctttatta ttcctacaga taaaattagt ctaactcaaa gtttgttggt tgcctacgat
    82801 atgctgttcg aatgggctgg agtgccatgg cttggctctg tgatggcatt tatgctagca
    82861 attggcgtac tgggtggggt ggttacttgg attgcagggc ctaatacagg tgtattagcg
    82921 atcgcgaaag ctggttattt acctaagttt ttccaaaaaa ccaatcgtca tggaatgggg
    82981 caccatctaa tgtttgtaca aggtataatt gtctcagttc tttctgttac ttttgtgata
    83041 atgccttcag ttcaagcagc ttttcagatt ttatcacagt taacagttat gctctattta
    83101 gttatgtata tgctcatgtt cgctagtgct atctatctac gacattctca acctgataca
    83161 aaacgcccat atcgagcccc tgctttattt ttctgggcaa cacttggatt cttagggtct
    83221 tttctggctt ttgtattaag ttttattccg cccgagcaaa tcccaactgg tagtccaacc
    83281 agctatgttc tttatttgtt agtattagtt gccctatttt tattaattcc aatattaatt
    83341 tacgccttcc gaaaatctga ctggaaagat ccaaactctg atttcgaacc attttcttgg
    83401 gaaaaaagaa ctagtaatta aattttccat aagctacaaa agatgaggtt aaatatgttt
    83461 agtgctgaat ctccaattgg tgtaatagac tcaggtgtag gtggattgac atcggtaaag
    83521 gaaataatta atttgcttcc tggtgaagat attatctatt gtggagataa tatgaatgca
    83581 ccttatggca accgttctgc cgatgatatc atcagtttga ctaaaaaaat gctgactttt
    83641 ttacagtcaa ggaatgtcaa actagttgct gtcgcatgta atactatatc ctcaactttg
    83701 gaatccgaaa aatacagcgg atatgctaag agctttccgt ttcctatctt atccatcatt
    83761 gaacctgcag tagaagatgt gattcgccag caatataaaa atgttggtat tatcgctacc
    83821 gagtttacta ttaaaacggg gtgtcacaaa gagctaataa aaaaattgaa ttcaacaata
    83881 aatgtatttg gagaaccaag caaaaatctt gcaatgttaa ttgaggaagg taatcttaat
    83941 gctcctgcaa tcttgaatga catcaaaaaa catgttaacc atttaatttc cctccatcca
    84001 gtaaatgaaa ttattctcgg ctgtactcac tatccaattg ttcagaattt ttttgaagca
    84061 gttgcaccgg atataaaatt tattaatcct gcacatgatc aggctatctc tattaaaaat
    84121 cacttgggac agctaaattt attaaactcc tcaaatatag gaactttgca aataaatact
    84181 agtggaagta tggagatcta caaaacggta ctaggggaat tgagcattac gaaacctcat
    84241 acattcagta tcaggcagtt ttaaatttat ccggtgcacc gtaaaatccc gtccttctga
    84301 actgcacccc aaaagttgga caccaactga gtaaggtgca ttttatggac aaacccagtt
    84361 acatactggg tagtgcatcc aattagtaga acatgtgttt ttcgataaac gctccgatca
    84421 ctttttcgtg gatctcaact gagcgtgaga agcagattgt tttacgaccc aaccgcttaa
    84481 tgcgggtgcg tagcgtcaga ttattacgct caatgcgttg ggtgaatatt ttgccggtca
    84541 gatgcttatt cttcggcacc tcccggccat agctgcccca gtcatcgctg gtcagcatgc
    84601 cgatgttgaa gggtgtaagc agtgccagta gctcccggca cgtttgatcg gttcggggac
    84661 caaaagtgta ggccagtaca ccgcctgttt tggtgttgta cgcgtacccg agccaatgtt
    84721 gccgggcttt actgccaacg tagctccatt gctcatcaag ctcgcagata aacgccacat
    84781 cagcatgggc aacaggcgaa gaagttattc gctttggcgc gagtttttta aagtccggat
    84841 gacggtgtta ctaccaattt tcagtgtcct ggcggtatcg cgaaccccgg caccattaaa
    84901 ggccatttca gttatcagct ctttcatacc cggcttacgt gcttgataag tataagtgaa
    84961 ctgaaacaca cggtggcagt cacggcagcg aagtctgtca cggcctttag ggttctgacc
    85021 atggcggtaa acctgagctg actgacaacg gggacaatga atattaacgc tggtcatgag
    85081 ataacctcaa aagcccgtat tatacatcag attcaactaa ttagaggcat cacccatcca
    85141 gcattataaa acatactaac aataactaaa ttataataat cacaacttac aatcaaataa
    85201 taaatgcata agattaacaa tgattttttc gggttatatt tatctaaaaa agcagaatat
    85261 tatgtaactt attattttct aagcgaaaat ttattgtgcg gtcagactaa caaacttgcg
    85321 taccacccga agaacatgcg aagatttaag tattctttct tttttggttt ttatttttca
    85381 ttttctcttt tcttctcttt ccggttctct tctgttctgg ttttggtttt ttccttttta
    85441 tttttattct ttcttcaggg ttatgctaaa acaccaaaac atttttcacc acaaaagcat
    85501 ccaatcatct tgccagacca atcaccttaa ccggatacac tgccggatat aatcccacta
    85561 acacccagcc accaagcacc atacctgata actaccgtat tcaggaagga aatgcgagtg
    85621 atttcaccgt caccggcaca accgatccgc caaaaagagg cgtaccaatg tcgatatagt
    85681 cccccgcgcg gacaatcact tcgtcaatga ctgccaacgg gagttgttgt agctgtgggc
    85741 gcaacaacat gcccagagct ttaccaaagt cctgcccggc caccaccagc aggggatgcg
    85801 gattcggaaa acgcgcgacg aaatcgacca gcgcgttgat gaccgtcagt accgcggcgt
    85861 aacgcacagg cagcgaggcg ggaagcgcca gcacgtacac gtcagttttg ggatcaagat
    85921 ccagctgaag cagcgcctgt tgccaggcac tcaccagatc cgtttcatca atcgggatcg
    85981 ccaccggcaa attgcgcagc ggcagttgta cgccctccag ccagattgtg ctgccagaga
    86041 gcgaaagggt atgtgcaccc gcgccaatca ccgtggcgca tacggtttgc gccggaaact
    86101 gcacattcat ctcacgcagg cgcggatggt catgcagcgc cgttgccaga agtgggccaa
    86161 tatcggcaaa acagaacggg tcggcgggct ggtggcgata acattcgccc acgcctccag
    86221 aaagcgtaat gatttcgggc gtaacacctg cgggcagcaa accggtttgc atcaatgcct
    86281 gcgcgagcgg cgaaagcgtt ccgtcaatca cgtcgacaat cagcgccgcc atccgccggg
    86341 taacctgcac cagctgcgcg ccggtcagcg aacgggcgtc agtgcctgca ccgaagcact
    86401 catccacaat catctgcccc ggtttatgag cgtaaaccac gcgcccctgg ctgtcggttt
    86461 ccagcaggcg accaccgacg ttgagacagg cagtgccgct gatttttccg gcatcgaaca
    86521 gggcgtagtt cgcggtgcca ccgccgatat cgatattcag tacccgacac agccgttgtt
    86581 cagaaagggt ttgcgccccg gctccgtgac cggcgatcac ggattcgagg tgcggcccgg
    86641 cgctggcgac gacaaaatcg cccagcgact gtttttcctt catcactttt tccatcagct
    86701 gtgcgaccag gctttcggta aactggcctt ccggcagctg ggcaatgatg gtttcgcgga
    86761 tgcgctggct ttcggttttg tcggctggca cgctggcggg cacggacgcc gttaccggga
    86821 tcgattcatc acattcggtg atggtaaaac ccagtagatc cgacacttca cgggcttccg
    86881 gggtaataat gctggcgcgc agaaccacgg acattgcctg ttcgccgcgc gcgtgtgctt
    86941 cacgaatatc attcgctgtg atgagttttt tcacctgctt cctcctgttt ggtgagagat
    87001 gccagataat ccaccagttg ctgcacgctt tgcgggtcgt ggctgtttag ctcgaaaatc
    87061 ggctcttcga aacccgtctc aagcaataac tttcgcgttg cggcgacgtc agcatctggc
    87121 atgtccgttt tactgatgac gacgatttgc cgtttgctga cgccaatatc cagcaacccg
    87181 gcaggcaacc gactttccgg gtcatttgcg ccatggacat agatcagcat gtctacatcc
    87241 tgtagcgtgg taattaaggc gtgataccag cggggatggc taaaatattc acccggcgta
    87301 tcaatatcgc ccttatcatt aaattccacg gcctgtgttt ttctggcgag ggtataattc
    87361 ccctgcaacg cattaaataa cgttgttttt cctgccccga cgctacccac aaacgcaatt
    87421 cgtttcatga atgcctcgta ttaacttttg gtcatttcac aaagcgtata atttaataag
    87481 cgacctaaac cgctgacggt ttgtgataac gcttcttcta ctgcgcccac cgagccataa
    87541 atcaccaatg caccgctgaa gcggtcgaga aaaccgatat gcacatcagc ggctttcagc
    87601 gctaaatcac cggcgatcat cgccgtttcg ccgggagtta gcgtcataat gccaattgcg
    87661 cccgcatccg gaacgccgat tttcttcgcc agttcctcac cgggatgcgc gatgagatgc
    87721 gccagtgtga cctgtttgcc gggcacaaat tcctgaatga tgcgttcttt atccatgagt
    87781 cgccgcctgt gaaaaacttt cgtgtatcgt gacaattttt cgtcagagta aataacaaaa
    87841 ttcggcaagc gcgtttaaaa ggtgaggtag atcactaaga atggagagat atttactgtt
    87901 atgaataaac tttcatatgc gacgcatgaa tataaataaa ataatatcga gatattttat
    87961 atactgattt ttttattatt cgcaaactca gtaataagct ttgctaatat atttcaggaa
    88021 aaccgcttac cctgaatatt cagggtaagc gtgagagtta agaatgaatt acagcggttg
    88081 ggtttgcgct tcaaccacgg ccagcgccac catattgacg atacgacgca ccgatgcgat
    88141 cggcgttaac acgtgaaccg gtttcgccac gcccatcagc accggaccga cagtcacacc
    88201 ttccgagctg gaaacgcgca gtaagttgta actaatgcgg gcagcttcca tgttcggcat
    88261 caccagaata ttggcagaac ctttcaaagg gctgtccggc atacggtcgt tgcgaatcgc
    88321 ttccaccagc gctgcatcgc cgtgcatttc accatcaatc atcagatctg gcgcacgatc
    88381 cctgaccagt tccagcgcct gacgcatttt gctcgacgac gggcagtcag aagaaccaaa
    88441 gttggagtgc gacaacagag caacgcgcgg ctcaatacca aaacgacgga cagtttctgc
    88501 cgccatcaag gtgatctccg ccagctcttc tgcgtctggt tcgtcattga cgtaggtatc
    88561 ggcaacaaag gtgttaccac tcggcagcag cagcgcgttc attgcacctg cggtgtgaac
    88621 gccatcgcga taaccaaaga catttttcac cacgctaaaa tgttcatgat aatcgcccac
    88681 cgtaccgcag atcattgcat cggcttcccc acgctgaacc atgatcgcgc cgatcactgt
    88741 cgggttactg atcagcgcac gctgcgcctg ttcctgagtg acaccgcgac gcttcatgat
    88801 ctggaagtat tcggtccagt actctttaaa gcgcggatcg gattcgttat tgacgatttc
    88861 aaaatcaacg cccgctttga tctgcaagcc cagtttctga atgcgcattt cgatcacgtt
    88921 cggacgaccg ataaggatcg gtttcgccag tcccagcgtt accagttcct gagtcgcatg
    88981 cagaacgcgc gcctcttccc cttccggcag aactacgcgc ttcggcgctt tgcgagcctg
    89041 ggagaaaatc ggcttcataa acaggttggt tttgtaaacg aactcagtca gcttgtcgat
    89101 gtagacgtcg aaatcagcaa tcggacgcgt cgccacgccc gactccatcg ccgcttttgc
    89161 gaccgcagga gcgatcttaa cgatcaagcg cggatcaaac ggttttggaa tgatgtattc
    89221 cggaccaaag ctcagatcct gaacgccata cgctgaagcc accacttcac tctgttctgc
    89281 atgggccagt tctgcaatcg cgcgtaccgc cgccagtttc atctcttcgt tgatggcggt
    89341 tgccccaacg tcaagcgcgc cacggaagat gaacgggaag caaagaacgt tgttcacctg
    89401 gttcgggtag tcggaacggc cggtacagat gatggcatcc ggacgcactt ctttcgccag
    89461 cggcggcaga atttccggtt ccgggttcgc cagcgccagg atcattggtg cccgagccat
    89521 tttcttcacc atttcctggg tcagcacttt cgggccggaa cagcccagga aaatatccgc
    89581 accttcaatc acatcatcga gggtacgttt gccgtcatcc accaccgcat atgcggcttt
    89641 ggtttccgcc atatttggct cacgcccctg atagataacg ccttttgaat cgcaaaccac
    89701 gatgttatgt ttttgcagcc ccagcgctac cagcaggttc atacaggcga ttgctgcggc
    89761 acccgcgccg gaaaccacca tccgcacgtc ggagatgttt ttctccacca cgcgcaagcc
    89821 gttgaggatg gcggcagtgc tgatgattgc cgtgccgtgc tgatcgtcgt ggaataccgg
    89881 aatattcatc cgctcgcgca gtttctgttc aatatagaaa cattctggcg ctttaatgtc
    89941 ttcgaggttg atgccgccga aggtcggttc gagcgcggcg acgacttcaa taaatttgtc
    90001 cgggtcgagt tcatcaactt caatgtcaaa tacatcaatc ccagcgaatt tcttaaatag
    90061 aacgcccttg ccttccatca ccggtttgcc tgccagcgcg ccaatgttgc ctaaccccag
    90121 caccgccgta ccgttagaga tcaccgcaac caggttaccg cgcgcggtat atttgtaggc
    90181 ttttagcggg tctttttcga tttcaagaca aggtgcggca acgcctggtg agtaggccag
    90241 cgccagatcg cgctgcgttg ccagaggctt ggttggagaa acctggattt tccctggaac
    90301 tggaaattca tggaaatcaa gtgcactttg ttttaactgg tcatccattt ggttgttcct
    90361 ttcacgtaac gttcacaaat aaagtgtgtg ggcaacagcc cctgcccaca acgtggcgca
    90421 cattattacc ctgccggagt ctacagactt tgagcaagtc caaactctca ccattaatat
    90481 aatgttttgg taacaatcct ataacactga tgttacctgc ttaatccagc aataccatgc
    90541 ctgtctgcta tgcttttttg atgcgtttag cgaaatttct cagaagtgtg aattaacgca
    90601 ctcatctaac actttacttt tcaaggagta tttcctatga acgagttaga cggcatcaaa
    90661 cagttcacca ctgtcgtggc agacagcggc gatattgagt ccattcgcca ttatcatccc
    90721 ctggatgcca ccaccaatcc ttcgctgtta ctcaaggctg ccggattatc acaatatgag
    90781 catttaatag acgatgctat cgcctggggt aaaaaaaatg gcaagaccca ggaacaacag
    90841 gtggtcgcag cgtgtgacaa actggcggtc aatttcggtg ctgaaattct caaaatcgta
    90901 cccggttgcg tgtcaacaga agttgatgca cgcctctctt ttgataaaga aaagagtatt
    90961 gagaaggcgc gccatctggt ggacttgtat cagcaacaag gcgttgagaa atcacgcatt
    91021 ttgatcaaac tggcttcgac ctgggaagga attcgcgcgg ccgaagagct ggaaaaagaa
    91081 ggtattaact gcaacctgac gctgctgttt tcttttgccc aggcgcgggc ctgtgcggaa
    91141 gcaggcgttt ttctgatttc gccgtttgta gggcgtattt atgactggta tcaggcacgc
    91201 aagccgatgg acccgtatgt ggtggaagaa gatccgggcg ttaaatcggt gcgcaatatc
    91261 tacgactact ataagcaaca ccactatgaa accattgtga tgggcgcgag cttccgtcgc
    91321 accgaacaaa tcctcgcctt aaccggctgc gatcgactga ctatcgcacc gaatttgctg
    91381 aaggagctgc aggaaaaagt ttctccagtg gtacgtaaat taatcccgcc ttctcagacg
    91441 ttcccacgcc cagcacccat gagcgaagcg gagttccgtt gggagcacaa tcaggatgcg
    91501 atggcggtag aaaaactgtc tgaaggcatt cgtctgttcg ctgttgatca acgcaaactg
    91561 gaagatcttc ttgccgccaa actataaacc agccacggag tgttatatgt cccgaaaaga
    91621 ccttgccaat gcgattcgcg cactcagtat ggatgcggta caaaaagcca attctggtca
    91681 tcccggcgcg ccgatgggca tggctgatat tgccgaagtg ctgtggaacg attttcttaa
    91741 acataatcct accgacccaa cctggtatga tcgcgaccgc tttattcttt ccaacggtca
    91801 cgcgtcgatg ctgctctaca gtttgctaca tctgaccggt tacgacctgc cgctggaaga
    91861 actgaagaac ttccgtcagt tgcattcgaa aaccccaggc cacccggaga ttggctatac
    91921 gccaggcgtt gaaaccacca ccggcccgct tggacaaggt ttggcgaacg ccgtcgggct
    91981 ggcgatagca gagcgtacac tggcggcgca gtttaaccag ccagaccatg agatcgtcga
    92041 tcacttcacc tatgtgttta tgggcgacgg ctgcctgatg gaaggtattt cccacgaagt
    92101 ctgttcgctg gcaggcacgc tgggactagg caagctgatt ggtttttacg atcacaacgg
    92161 tatttccatc gacggtgaaa cagaaggctg gtttaccgac gatacggcaa aacgttttga
    92221 agcctatcac tggcatgtga tccatgaaat cgacggtcac gatccgcagg cggtgaagga
    92281 agcgatcctt gaagcgcaaa gcgtgaaaga taagccgtcg ctgattatct gccgtacggt
    92341 gattggcttt ggttcgccga ataaagcagg taaggaagag gcgcacggcg caccactggg
    92401 ggaagaagaa gtggcgctgg cacggcaaaa actgggctgg caccatccgc catttgagat
    92461 ccctaaagag atttatcacg cctgggatgc ccgtgaaaaa ggcgaaaaag cgcagcagag
    92521 ctggaatgag aagtttgccg cctataaaaa ggctcatccg caactggcag aagagtttac
    92581 ccgacggatg agcggtggtt taccgaagga ctgggagaaa acgactcaga aatatatcaa
    92641 tgagttacag gcaaatccgg cgaaaatcgc tacccgtaag gcttcgcaaa atacgcttaa
    92701 cgcttacggg ccgatgctgc ctgagttgct cggcggttcg gcggatctgg ctcccagcaa
    92761 cctgaccatc tggaaaggtt ctgtttcgct gaaggaagat ccggcgggca actacattca
    92821 ctacggggtg cgtgaatttg gcatgaccgc tatcgccaac ggcatcgcgc accacggcgg
    92881 ctttgtgccg tataccgcga cgtgcctgat gtttgttgaa tacgcccgta acgccgcgcg
    92941 gatggcggca ctgatgaaag cgcggcagat tatggtttat acccacgact caattggcct
    93001 gggcgaagat ggtccgacgc accaggctgt tgagcaactg gccagcctgc gcttaacgcc
    93061 aaatttcagc acctggcgac cgtgcgatca ggtggaagcg gcggtgggct ggaagctggc
    93121 ggttgagcgc cacaacggac cgacggcact gatcctctca aggcagaatc tggcccaggt
    93181 ggaacgtacg ccggatcagg ttaaagagat tgctcgtggc ggttatgtgc tgaaagacag
    93241 cggcggtaag ccagatatta ttctgattgc caccggttca gagatggaaa ttaccctgca
    93301 agcggcagag aaattagcag gagaaggtcg caatgtacgc gtagtttccc tgccctcgac
    93361 cgatattttc gacgcccagg atgaggaata tcgggagtcg gtgttgcctt ctaacgttgc
    93421 ggctcgcgtg gcggtggaag caggtattgc cgattactgg tacaagtatg ttggtctgaa
    93481 aggggcaatt gtcgggatga cgggttacgg ggaatctgct ccggcggata agctgttccc
    93541 gttctttggc tttaccgccg agaatattgt ggcaaaagcg cataaggtgc tgggagtgaa
    93601 aggtgcctga tggtgatcgc cggatgctgt atgttcccct caccctaacc ctctccccag
    93661 aagggcgagg ggacggtccg tgcgcttttt agggccgcat ctgcgatttt gtttaacggg
    93721 tgatccacaa cgtgggcctg gcgtctggcc catgccagtt atcgcaggta ggttctgcgg
    93781 cgtaacgcac caggcgaaaa cgctgaccgt caaagcgcca gcgcgcctga atgccacaat
    93841 cgcttaatcc gcgccctttc gctaaggtca ccagttcacg cgatttctca tcaaatgttg
    93901 cgttcatcag ttccagttca ttcgtctcct gaccgttgtt gaacggcaaa cgcaaccgaa
    93961 ccgggcgcga ggctagcggc tttttacgcg acacaatcca tgccaaatca atggtgttat
    94021 aggcccctgc ctcacagcta atcatcatca gtgctttatc atcggtcagc gcagtgacat
    94081 tcacctcacg acgtaacgga tcaagcgagc agcgcagacc attcatccgc cagtttccat
    94141 aatccagcaa atcgttacgt tcttcgagtg agagtggcgt cggcgttggg ttaaccaccg
    94201 cgacctcttt cagcgcaggc gcgggcggta cgctgagagg cggttcgtcc cctttcttga
    94261 tccacgcggt ttcactgcca acgcgctttt gctgagcatc aataaacaac aacgccgctt
    94321 ttaagccgct cagagaaatg gtctgattgc cgtcgcgtaa ggtgatcgcc ttcccttcct
    94381 gaattatctg caaaaacgcg gtgatggttg ccgtatcatc ggttactaat aaccatggtg
    94441 gaatccgcca cttgtcacca cttaatgcca acggctcacc atctaatagc agccgtggcg
    94501 ctatctcccc ttcagacgca tccggcgact tcaacccacc gcgctcaata cgtaaaacgg
    94561 catcggtatg cgccccagcg ctgcggctca gggtcatcac cagtccatta tgatcgcccg
    94621 tattgcgcgc cacacaaaaa ttttgattat tacaggtgac ctgccagtcg gaaaacgccc
    94681 gttgcgctgg tgccgcccac accaaagacg tcggcaacaa agcgaaaaaa agagaaggaa
    94741 aatgcgatag cgcatggacg gtacgacccc agaagttcaa acaaacagca caagtcgtat
    94801 cttcgttgcg caggtagcgc ggctcaatcg gatttgtcgg attgagccaa taataaacaa
    94861 cgaaatatta ttgctatttt tcagtccatt aaatgtgaca tttgcaaata gttaagcaat
    94921 aacaccgtct taccgtcacg tatctcgccg gttttgatca tctccagcgc ctggctgaac
    94981 ggcagctcga gcacttcaat atcttcatct tcaacaccgc caccggcgtt agcgcgctga
    95041 ttgtcactgt attcggcgat aaaaaagtgg attagctcag tcacaccgcc aggcgacata
    95101 tacagttcaa ataatttgcg cacttcgccc acttcatagc ccgtctcttc aatcgcttct
    95161 ttacgaatgc acacttccgg ttcgtcgtta tccagcagcc cggcgcaggt ttcaatcagc
    95221 tgcccgcttt cattgccatt aacccaggta gcgacacgga actgacgaat cagaaccacg
    95281 ctctttttct tcgcgttgta caggaggatc gtcgcaccat taccgcgatc gtaaacttca
    95341 cgtttatggc ggataacttc accatctttg cgcgtgagat cgtaagtaat gttgtgcagg
    95401 gtgaaatagt tatcggagag aattttgtct ttaatgaggg tgatttgttg cgtcataccg
    95461 actccacagc gcgaaatgaa caattatatt acgctgtgaa gtcgggtttg tctgcgcagg
    95521 ctatcagtga gatttgactg attttacccc cagccagtca ataatcccct gtgccgcatg
    95581 acgtccttct gccattgcgg taaccaccag atccgcacca cgcacggcgt cgccaccggc
    95641 gaagattttc ggattggtgg tctggtaacg gtactggctt tccacatccg cgatgatgcg
    95701 gccccatttg tctaccgtta caccgtgcga ctccagccac ggcatcccgt gtggattgaa
    95761 gccaaacgcc ataatcaccg catcggctgg catgacaaat tcactgccct caatcggtac
    95821 aggtcgccga cgcccctggg catccggctc gccaagacgc gtgcgcagga aacgaattcc
    95881 gcaaacgtga ccttgctcat tcagctcaag cgccaccggc tggacgttaa attcgaagtt
    95941 ggccccctct tcgcgggcgt tcttcacctc tttcttcgag cctggcatgt tagcttcatc
    96001 acgacgataa gcgcaggtga cgttactcgc gccgtggcgc agcgcagtac gcacacagtc
    96061 catcgcggtg tcgccgccgc ccagtaccac gacgttaagt ccggcggtat tgataaacgg
    96121 ctcttccggt agctcttcga gccccatcac ctgtttagtg ttggcaatga ggaacggcag
    96181 cgcgtcataa acgcccggcg tatcttcatt gggtaaaccc gctttcatgg aacggtaagt
    96241 gcctacgcca acgaagaccg cgtcgtattg ttccaaaagc gaatccaaag agacatcttt
    96301 acccacttca caattgagtt cgaagtgaat ccccatcgcg ctgaagattt cccgacggcg
    96361 tgccagcagg gatttatcca gtttgaaaga aggaatgccg aaagtgagca agccaccgat
    96421 ttctggatgg cgatcgtaca ccgtcaccgc cacgccattg cgggtcagaa catccgcaca
    96481 ggccagccct gccggacctg ccccgataat cgccacccgc ttgtccactt tggtgacatg
    96541 acttaagtca ggacgccaac ctttcgccaa cgcctgatct gaaatatagc gttcaatgtt
    96601 gccgatagtt accgcgccgt gctcatcgcg aatagtacag gcaccttcac acaaacggtc
    96661 ttgcggacaa acgcgtccgg taatttccgg cagggtgttg gtctggtgag aaagctcgac
    96721 ggcggcgtcg atgtttccgg ctttcaccag ttcaatccac tgcggaatat ggttatgcag
    96781 cgggcaggtc cattcacaaa cgctgtgctc gccgaactta aggcagcgcg aggcttcccg
    96841 ttgtgcctgg tcggcgcgaa atggcagata aatttcatca aaaccggttt tgcgcgcttc
    96901 aatcgccagt ttatccggct cgccacgcgc gggcgttgcc tgcatttgtt cgactttact
    96961 cattaccggc atttcttgtg cagcggtact ggcatgccac ggttgatgct cctgacgtgc
    97021 ggtgcgcaag cggcgggatt tcgccatgcc ggagagtgcg acgtcagtga ccagttgcag
    97081 cgcgtccgcc gggcaattct cgacacaggc aggaccgttt tcgcgccctg cacaaaggtc
    97141 gcatttatgc gccgtggctt ttacttttcc tgccgcgacg ggtgtcagga cgatttgcat
    97201 cgtaccaaac ggacaggcca ccacgcagga tttacagcca atacactttt gctgattgac
    97261 ctgaatgctg tcatcaacgt gactgattgc accattaggg cagctacggg cgcagggcgc
    97321 atcttcacaa tggtgacagg tcactgcact acgttgctgc tgatgtttga taaccgtaat
    97381 tcggggatga aaatggtgtt ggctcaggac atgttgctca tcattgtgag ccatgacaca
    97441 ggcgatttca caagcatgac aacccagaca ttgctgactg ttggccataa taaaacgatt
    97501 cataacgacc ttcttttttg gttgtaaaaa ccttattctt tatatgagtg ttgttattac
    97561 ccgacttaca ggggaaccgg caatgttcat gtgcccagaa taagtaacta tttcgctata
    97621 aactgtggca gatcaaataa tcccatcact gactaaattg cgtttcagcg aactggaaca
    97681 ttaatgattt tttgtggaga agacgcgtgt gattgttaaa cgccccgtct cggccagtct
    97741 ggcccgggcc tttttttaca ttgtgctgct gtcgattctt tccacgggta tcgctctgct
    97801 aactctggcg agcagtttgc gcgacgctga ggctatcaat attgccggat cgctgcgtat
    97861 gcagagttac cgcctgggct acgacttgca aagtggcagt ccacaactca atgcacatcg
    97921 ccagttgttt cagcaggcac tgcattcacc ggtattaacc aacctcaacg tctggtatgt
    97981 gccagaagca gtaaaaactc gctatgcgca tctgaatgcc aactggctgg agatgaataa
    98041 tcggctcagc aagggcgatt tgccgtggta tcaggccaat attaataatt atgttaatca
    98101 gatagacctg ttcgtgctgg ctttacagca ctacgctgaa cgcaaaatgc tgctggtggt
    98161 ggcgatttcc ctggctggcg gtatcggtat tttcacgctg gtctttttta ctttgcgccg
    98221 catacgccat caggtggttg ccccgctgaa tcagctggtt accgccagtc agcgtattga
    98281 acacgggcag ttcgactcgc cgccgctgga taccagtctg ccgaatgagc ttggtctgct
    98341 tgcaaaaacc tttaaccaaa tgtcgagcga gctgcataaa ttgtaccttt cgctggaggc
    98401 gtcagtagaa gaaaagaccc gcgatctcca cgaggccaag cgtcgtctgg aggtgttgta
    98461 tcagtgcagt gttcccaggc gctgaacacc agccagattg atgtgcattg tttccgccat
    98521 attttgcaga ttgttcgcga caatgaagca gctgaatatc tggagctgaa cgttggggat
    98581 aactggcgga ttagcgaagg gaagccaaac cctgagcagc caatgcagat tttaccggtg
    98641 acaatgcaag agacggttta cggcgaactg cactggcaaa atagtcacgt ttcatcatca
    98701 gaaccgctgc ttaacagtgt ttcatcgatg ctgggacgcg gtttgtactt taatcaggcg
    98761 cagaagcatt ttcagcagtt attgttgatg gaagaacgtg cgaccatcgc ccgcgaattg
    98821 cacgactcgc tggctcaggt gctttcttac ttacgtatcc agttgacgtt actgaagcgt
    98881 tcgataccgg aagataacgc caccgcacaa agtatcatgg ccgatttttc ccaggcgttg
    98941 aatgatgctt atcggcagtt acgcgagctg ttgactactt tccgcctgac gctgcagcag
    99001 gcggatctcc cctccgcgtt gagggaaatg ctggatacgt tacaaaatca aaccagcgct
    99061 aaactgaccc tcgactgccg tctgccaacc ctggcactgg atgcgcaaat gcaggtgcat
    99121 ttgttgcaaa ttattcgcga agcggggctg aatgcgatga agcacgccaa cgccagcgaa
    99181 atcgccatca gttgcgtcac cgcgccggac ggcaatcaca cggtttatat ccgtgataac
    99241 gggattggta tcggtgaacc gaaagaaccc gaaggtcatt atggactgaa tatcatgcgc
    99301 gaacgcgcgg aacggctggg cgggacgctg actttttcac aaccttccgg cggcggcacg
    99361 ttagtgagta ttagctttcg ctccgcagag ggtgagggga aggggaacac gccggctgag
    99421 gtgtaaataa ttaccctcac accggccctc tccctttaaa ggcgagaggg aaaaacgtgc
    99481 ccatattgac ctgtaagcga gtgtgtcagc aatctcatga taatttacat taactccttt
    99541 ttttctccac gattggctcg taccttgccg ctacagtgaa gcaagtcaag cctacaacaa
    99601 tacgcagaaa cacgaggtcc tcttttaatg gcgaatttct ttattgatcg ccccattttt
    99661 gcctgggtgc tggcaatcct gttgtgtctg acaggtaccc tggcgatttt ttcattgccc
    99721 gttgaacaat accccgatct cgcgccaccg aatgtgcgag tgaccgctaa ctatcccggc
    99781 gcatcggccc agacgctgga aaacaccgtg acccaggtta tcgagcaaaa tatgaccggc
    99841 ctcgataatc tgatgtatat gtcatctcag agcagtggca ccggtcaggc atctgtcact
    99901 ttaagtttta aagcaggcac cgatccggac gaagccgtgc agcaagtaca aaaccagctg
    99961 caatcagcca tgcgaaagtt accgcaggcg gtgcaaaatc agggcgtgac ggtgcgtaaa
   100021 accggcgata ccaacattct gaccattgcc ttcgtctcta ccgatggctc gatggataaa
   100081 caggatattg ctgattatgt tgccagtaat attcaggacc cgttaagccg cgtgaatggc
   100141 gtcggggata tcgatgccta tggttcgcaa tattccatgc gtatctggct ggacccggcg
   100201 aaactcaaca gtttccagat gacggctaaa gatgtcactg atgccattga gtcacagaac
   100261 gcgcagattg cggttgggca acttggtggt acaccttccg tcgataagca ggcgctcaac
   100321 gccaccatta acgcccagtc actgctgcaa acaccagaac agttccgcga tatcaccttg
   100381 cgggtgaatc aggacggctc agaggtaagg ctgggcgatg tcgccaccgt cgaaatgggg
   100441 gcggagaaat acgattatct tagccgcttc aatggtaagc cagcctccgg gttgggggta
   100501 aaactggcct ccggcgcaaa cgaaatggcg acagcggagc tggtgctcaa tcgtctcgac
   100561 gagctggcgc agtatttccc gcacggactg gaatacaagg tggcgtatga aaccacctcg
   100621 tttgttaaag cctccattga agacgtggtg aaaacgctgc tggaagctat cgctctggtt
   100681 ttcctcgtta tgtatctgtt cctgcaaaac ttccgcgcca cgctgatacc cactatcgcc
   100741 gtgccggtgg tgttgatggg aaccttctcc gtactttacg ccttcggtta cagcgtcaac
   100801 accttaacca tgttcgcgat ggtgctggcg atcggtctgc tggtggatga cgccatcgtg
   100861 gtggtggaaa acgtcgaacg tattatgagt gaggaaggac tcactcctcg cgaagccaca
   100921 cgtaaatcga tggggcagat ccagggggca ctggttggga ttgcgatggt gctttcggcg
   100981 gtatttgtac caatggcctt cttcggcggc accaccggtg ccatctatcg ccagttctct
   101041 attaccattg ttgcggcgat ggtgctgtca gtactggtag cgatgatcct cactccggct
   101101 ctgtgtgcca cgctacttaa gccactgaaa aaaggtgagc atcacgggca aaaaggtttt
   101161 tttgcctggt ttaaccagat gtttaaccgc aacgccgaac gctacgaaaa aggggtggcg
   101221 aaaattctcc accgtagcct gcgctggatt gtgatttatg tcctgctgct tggcggcatg
   101281 gtgttcctgt tcctgcgttt gccgacgtcg ttcttaccgc tggaagaccg gggcatgttt
   101341 actacctcgg tacaattgcc cagcggttca acccaacaac agaccctgaa agtcgttgag
   101401 caaatcgaga aatattactt catccatgaa aaagacaaca tcatgtcggt gtttgccacc
   101461 gttggttctg gccctggggg taacgggcaa aacgtggcga gaatgtttat ccgcctgaaa
   101521 gactggagcg aacgcgacag taagaccggc acctcgtttg ccattatcga gcgtgcaacg
   101581 aaggcgttta accaaattaa agaagctcgc gttatcgcca gcagcccacc agcaattagc
   101641 ggtcttggta gttctgcaga ttttgatatg gagttgcagg accacgctgg agcgggtcac
   101701 gatgcgctga tggcagcacg taatcagttg ctggcgctgg cggcggaaaa cccggagcta
   101761 acccgtgtgc gccataacgg cctcgatgac agtccgcagt tgcagattga tatcgaccag
   101821 cgtaaagctc aggcgctggg cgttgatatc gacgatatta acgacacact gcaaaccgcc
   101881 tggggttcga gctatgtgaa tgactttatg gatcgcggtc gcgtgaagaa agtctatgtg
   101941 caggcagctg cgccgtatcg catgctgcca gatgacatca atctctggta tgtccgaaat
   102001 aaagatggcg gcatggtgcc cttctctgct ttcgcgacct cacgctggga aacaggctcg
   102061 ccgcgtctgg aacgctataa cagttattct gcggttgaga ttgttgggga agccgcaccg
   102121 ggggtcagta ccggtacggc gatggatatt atggaatcgt tagtgaagca gctgccaaac
   102181 ggctttggtc tggagtggac ggcgatgtcg tatcaggagc ggctttccgg cacgcaggct
   102241 ccggcgctgt acgccatttc cttgctggtg gtattcctgt gtctggcggc attgtatgaa
   102301 agctggtcgg taccgttctc ggtaatgctg gtcgtgccgc tgggggtaat cggcgcgctg
   102361 ctggcaacct ggatgcgcgg gctggaaaac gacgtttact tccaggtggg cctgttaacg
   102421 gtcattggtt tatcggcgaa aaacgccatc ctgatcgtcg agtttgctaa cgagatgaac
   102481 caaaaaggcc acgacctgtt tgaagcgacg ctccacgcct gccgtcagcg tttacgcccg
   102541 attctgatga cctcgctggc atttatcttc ggcgtattgc caatggcaac cagcacgggt
   102601 gccggttccg gtggtcagca tgcggtgggt actggcgtaa tgggcgggat gatttcagct
   102661 actattctgg ctatttactt cgtgccgctg ttctttgtgc tggtgcgccg ccgcttcccg
   102721 ctgaagccgc gcccggaata agcaataaaa aaggcgacat gccaatgtgt cgcctttttc
   102781 aactttccga ttaagaacct gctcagcggg ttcttgctgt ttatactttg tctcaggaat
   102841 tacttacgaa gcataacttc gataaagtct ttccagttcc ccagttcacg ttcaatcata
   102901 acaacctctc ttataattat gggtattcta cggaaaaaat ataccgtggt gaagctaatt
   102961 tactcgattg ctgcgatgac tacctccggg ggacaaacct tatgtaaata ctatggtcct
   103021 gcggtgataa tttgtatgtg atacacagca acatttcgag atattcatac ggcatctaat
   103081 acttatttaa ttctggttaa aatacagaca gataacaaga tgaatattct taatgtttac
   103141 gttaaaaatg tttaatatta tttaatagtt gttaatttga atacttcgat aatgttatat
   103201 tttctgataa tcatttgcag gcaaaatgtt ttcaccctta aatgagtatt tattctcata
   103261 aatcgaaaaa ggattcatta tggttacact ttacggcatc aaaaattgtg acaccattaa
   103321 aaagactcgc cgttggctgg aagccaataa catcgactat cgttttcatg attaccgcgt
   103381 cgatgggctg gacagcgaat tattgaacgg ttttatcaac gaattaggct gggaagcgtt
   103441 actcaacacc cgtggtacaa cctggcgtaa actggacgaa actacccgca ataaaatcac
   103501 cgatgcggcc tctgcggcgg cattaatgac tgaaatgcct gcaattatca aacgtccatt
   103561 gctctacgcg cctggtaagc ctatgctgct gggtttcagt gattccagtt atcagcaatt
   103621 tttccatgag gtgtagtcta tgtcgtgccc ggttattgag ctgacacaac agcttattcg
   103681 ccgcccttcc ctgagtcctg atgacgcagg atgtcaggct ttgttgattg aacgtttgca
   103741 ggcgatcggt tttaccgttg aacgcatgga ttttgccgat acgcagaatt tttgggcatg
   103801 gcgtgggcaa ggtgaaacgt tagcctttgc cgggcatacc gacgtggtgc cgcctggcga
   103861 cgccgatcgt tggatcaatc cgccgtttga acccaccatt cgtgacggca tgttattcgg
   103921 gcgcggtgcg gcagatatga aaggctcgct ggcggcgatg gtggtagcag cagaacgttt
   103981 tgtcgcacaa catcccaacc atacggggcg actggcattt ctgatcactt ctgatgaaga
   104041 agccagtgcc cacaacggta cggtaaaagt cgtcgaggcg ttaatggcac gtaatgagcg
   104101 tctcgattac tgcctggtcg gcgaaccgtc gagtatcgaa gtggtaggtg atgtggtgaa
   104161 aaatggtcgt cgcggatcgt taacctgcaa cctcaccatt catggcgttc aggggcatgt
   104221 tgcctaccca catctggctg acaatccggt acatcgcgca gcacctttcc ttaatgaatt
   104281 agtggctatt gagtgggatc agggcaatga attcttcccg gcgaccagta tgcagattgc
   104341 caatattcag gcgggaacgg gcagtaacaa tgttattccg ggtgaactgt ttgtgcagtt
   104401 taacttccgc ttcagcaccg aactgactga tgagatgatc aaagcgcagg tacttgccct
   104461 gcttgaaaaa catcaactgc gctatacggt ggattggtgg ctttccgggc agccattttt
   104521 gaccgcgcgc ggtaaactgg tggatgcggt cgttaacgcg gttgagcact ataatgaaat
   104581 taaaccgcag ctactgacca caggcggaac gtccgacggg cgctttattg ctcgcatggg
   104641 ggcgcaggtg gtggaactcg ggccggtcaa tgccactatt cataaaatta atgaatgtgt
   104701 gaacgctgcc gacctgcagc tacttgcccg tatgtatcaa cgtatcatgg aacagctcgt
   104761 cgcgtgatga gtggttctgc aagaggaaat aagcatggac tggctggcta aatattggtg
   104821 gattctggtg attgtctttt tggtcggcgt cctgctgaac gtgattaaag atctcaagcg
   104881 cgtcgaccat aagaaatttc tcgccaacaa gccggagctt cccccgcatc gtgatttcaa
   104941 cgataagtgg gacgatgacg acgactggcc gaaaaaggat caaccgaaga agtaagctga
   105001 gtgctcaacc gtaggccgga taaggcgtta acgccgcatc cggcaatggt gaacgatgcc
   105061 tgatgcgacg ctcacgcgtc ttatcaggcc aggtaatctg tcttttacat catttcaatc
   105121 acatcatcat cgccaggctt accgccactt agcgcttggt cgaaataatg tttcggaatg
   105181 gtataacgca agtgatccag ggcaaattgc atactgcgat tgtcgatcgc atggcctaaa
   105241 tcctccacga tatccagcgt cacgtcacca ccggcactga ttaacgcttc ctgcgccgca
   105301 actgcatgcg ccagatcgat aaccggatct tcaccaccat gaatcaagtg aattgtcgtg
   105361 gctgtagacg cggtctccgg caggctcgca tagcgtccgt taaaggcaat gacgcgcgaa
   105421 gcaaggcccg gttcggcttt aatgctctcc agcgccataa tcgcgccttg tgagaaacca
   105481 attagcgccg tggcgttagc ccctactccg ctctgtttct gccagtagcg taccgtttca
   105541 ataaacgtcg gcatgatggc atccacacgc gcctggcgat tatcttccgt aataccctgc
   105601 actgaaaacc actgacgccc cgccggatta ccgctcggct ccgcgccacc gacactcacc
   105661 accagcgcat caggaaacag cggtgcaaac cagctgccta tttcccccat tgccaccggg
   105721 ttatccccga caccgtggaa aagcagtaac aactgttgtg caggtttatc cgggctttga
   105781 acaacaaaat ggtcatgttt catggcgaac tccttaactg atgtcattaa ttttacgccg
   105841 ctgactcaca atgaccatgg gagaaaattg aaggagtcag tgaaaaaatt gccattgcgt
   105901 tatgcgatcg cgcagacgct cagtgcgaac ctcatttagt gcgaacagcg cctgcgcggc
   105961 ctcttcccgc tggcgaacca gtaacatctt gcggcctgaa agtttaagtg tggtacataa
   106021 ctgcgcgtca ctggcgtttt tctgtaaacg cccacgcagc gccggtaatg ccagttcact
   106081 agtttgtagc agacgcaata agcaacctaa cgatgttaat agcggacgat gagcgaaagc
   106141 aaaaccggcc agttcaagcc agtcgtcgtc agaaaggatg gcatcgttta gtggatcaac
   106201 aggaagcatt tcgccattcc actgcgcgag agcttgcgca tcgcgacgta aacggtaatg
   106261 ctcacgttca gccagctgtt tacccgcatc actcatcggt aacagcgcca tcgccgtata
   106321 gcaaccgctg ctggcttccc gatgattacc catccgcacc agcacaaaac cgcagcgttg
   106381 ccagaaacgc cataactccc cagtgtaacc aaaactcacc gaaagatagt cgaggtcatg
   106441 cgtatattgc aaagctccag caataagttt ccgccctgtg ccttcccgct gacgagccgg
   106501 atgaactgct atccggctaa cccgccttcc gcgcaatgtc gccgccagtg gattgctgcc
   106561 gtgcgccgcc agcgactggg ccaccagatt accccgcggg cgacgaaaac ctgcccatac
   106621 cgcctgactg agttcttgag ataatccgcc ctcatccacc agccacaacg ccccggcaat
   106681 ctcgttttcg ccagccgcct gtaaaaaatg ttgccctggt gcatccatca tccggcgtaa
   106741 atccagcggc gaggtccggt agtgcgcacc agacaataac tgataaacct ttaacggcgt
   106801 ttctggctcg cttcgccata acgtttgttc aaatgcggaa atgacgatat tgccttgtgg
   106861 tgtatgggtg aagttttcat cgttaaaaac cagtgcctca ctaaccattt tttccagcgg
   106921 gcatccctgt gcccagcgga tcggctgttg cagttcaaaa cggtgtaaat gcggaaagcg
   106981 agcgcaaaat ttcagcaaaa aaccacgtcc ggtaccttcg tagccctgca ccgtagtggt
   107041 taacaacgtt cgaggaaaac gcgataccag ttgatgcaac aacggcgcag gtatggctgc
   107101 ggcttcgtcg accaccagcc agtcggcttg ctcatcgctg gctaacaagg catccggtgc
   107161 aataaagcga aacttctcgc ccgcaaattg tgccagtaca tccgttgccg cttttgcggg
   107221 cgcggtgaca atcgcactgc ccgcaatacg agaaatgagt tgccctgcca acgccgactt
   107281 accgcgccca cgcgcagccg ttaccgctgc cacgcccggc ggcatggtca gtagctgctg
   107341 taagagttgc tgttgttctg gttgtggtgc gccggtcgcg gggtgccagt cagtacgggg
   107401 agtaaaatgc gccaacgaga acggctggtt ttgccgccag aggatagcgt cgttatccgc
   107461 cgtaagtacg cgtttgagat gctggacaaa atgcggcgtc gcaatagggt cagggcaatc
   107521 actccagcgc agcgagtcgg catcaggttg gttttcccac tcttcccata cagggagtaa
   107581 caaaaccagc cagcttcccg ctttcaacgt tccgctaagt gctgcaaagg cagcggcatc
   107641 aaagccgtgg cgggcgtcga ataccgcatg ccggaactcg cgcccaagta aagtttgtag
   107701 cgccgagggt gaacagtggt tttcagcatc tagctgcggc gaaatccaca gccagtcgcc
   107761 aggtaaggca tcacgcaact taagagcatg atcaaaacac caaccctctt ctccgctcaa
   107821 caccagcaag cggcagatcc cttcacgttt catttgcgct gttaatgtgt aaagcgcagt
   107881 cagttcagcc atccctgccc cggaaattaa atgcttttac caaaagtatt gcattgtgcc
   107941 ggatcgccgc tgtcgaaacc acgtttaaac cagctgcagc gttgctgaga agtgccatgt
   108001 gtgaaactgt ctggtactac tcgcccctga ctttgctgtt gtaaacggtc atcgccgatg
   108061 gcctgcgccg cattcagcgc ctcttccaga tcgccagttt ccagaacgcc ttgctgctgc
   108121 atactatgcc cccagacacc ggcaaaacag tcggcctgga gttccatacg cacagataag
   108181 cggtttactt ccgcctgcgt cgcgttttgt tgcagttgac gaactttcgg ctcgatgcct
   108241 aacagtttct gcacatgatg accgacttca tgggcgataa cgtacccctg ggcaaaatcg
   108301 ccatccgcgc caagtttgtc tttcatgtca tcatagaagg agagatcgat ataaactgtg
   108361 ccatccgccg ggcaatagaa cggccccata atggactggc ccgccccgca gccggtgcgc
   108421 gtcattccac ggtacatgac cagcttcggt tgctgatagg tcttacccat cttttcgaac
   108481 tgttgtcccc aggtgtcttc cgtggttgcc agaatcaccg aggtgaattt tgcggcttca
   108541 tcttcattag ggctaattga ccgcgttgat tgttgttgag aaaccggctg cccggtcatc
   108601 aacccggtta aatcaacgcc atagtagcct gcaaccagca cgactatcag taaaatcagc
   108661 ccgcctttac cgctcggcag acggaaaccg ggaccgccca ttgacggacc gccagagctg
   108721 ttgcgcctgt cttcaacatt gtcactttca cgtcgccctt gccaacgcat agatacctca
   108781 acaatatatt cattatagaa atgatcgtag gtggttcaga ggaagattac cacaggaaag
   108841 cgggatgaaa tggcggagga atgagcgttc aatgcatata ttccgagagc ggttgtcttg
   108901 ccgtgccagc tgcacggcaa gatgatgaac agaaaaatca gtccagctgt acacccaggc
   108961 ggtgggcgac ggcttcatag gcttcgatca ggccaccgag actctggcgg aaacggtctt
   109021 tgtccatttt ctccagtgtt tctttgtccc acaggcggct gccgtccggg gagaactcat
   109081 cgcccagtac cacttcacct ttgtacagac caaactccag cttgaagtcg accagaatca
   109141 gaccagcatc atcgaacagt tttttcagca cgtcgttcgc tttgtaggtc agctctttca
   109201 tacgcgccag gttctctttg ctcacccagc caaaggtttc gcagtaagat tcgttgacca
   109261 tcggatcgtg catggcatcg tttttcagga acagatcgaa cagcggcggg ttcagctcaa
   109321 taccttcttc gattccaaga cgtttcacca gagagccagc agcacggtta cgcacgacac
   109381 actcaaccgg caccatatcc agctttttca ccagacattc ggtatcggag agcagacgct
   109441 ccatttgagt cgggataccc gcttcagcca gtttgctcat aatgaagtag ttgaacttgt
   109501 tgttcaccat acctttgcga tcaaactgct caatgcgcgc gccatcccct gctgacgtat
   109561 cattgcggaa ttcgagcacc aacaggtccg ggttttccgt gctgtatacg gttttcgctt
   109621 taccacgata caactcagct tgcttttgca tctttatcac tcctgggtgt gaattaacgt
   109681 tttaaaatct tttgctgtct ggtgtgccgg atgttttttc ggatgcggcg tgaacgcctt
   109741 atccgaacta cgtccgacaa acaaatttcg tgcgaattac cgctaaaatc gcgttttcct
   109801 gccgacgcac acgtttgcgt atcatatcag aaaaaagggc cggatgattc cagccctgta
   109861 tttttacttg ctaaacgcag cctggaagac agctaccagc gcgtcgttct gactctgagt
   109921 cagagtatga cctttcggat cgatgaactg taggctgctg cggttatcta aatcgccaac
   109981 ctgcagttta tagtcaccgg atgccaggcc tggatcgctc gcgcccagtt cctgccagtc
   110041 gctgtcagac agcggcttat aagttacggc catgttgccc tgcgaacggg tgctgtcggt
   110101 cactttcatg cccacttttt ccagcgacgc tggcagacgt tgccaaacca cattgaacgg
   110161 cccgcgtacg accagcattg gtaaaccggt gtcatcagct gcactttgta cgtccatagt
   110221 ggtggaggca cgattttgcg cagcgttcgc ggcgtcagtg gcagatttat ccagaccggc
   110281 ggaaataacg ttcatcatct ccgtgctgta acgctgcatg gaagccgcgt ctgcaaccgg
   110341 tttgcccgcc tgttccaggt tcagcagttt aaccgtaacc gcctgctgat aaccctgcgg
   110401 cttaacagag atttgataac gaccacgata ctgctcgtct tcgtccagac ggttccattg
   110461 tacccaatcg gtggtcagtg tctgaccagc atcatcacgt tgggtgatgg tgtagttttt
   110521 cgcctgcagc acgctaacca cctgcggcca cagagtattg ccacgaccat tttccaccag
   110581 caatgaagcg gtatcgcccg tgaactgggt acgcgcgcca gaaaccagtg ccagcggctg
   110641 ggctggtgga cgaatgtcca gcgccttacc gacagcacca ctaccgttgg tcaccgggat
   110701 tgcataatca ccggaggtca ccggcaaaat cattccagcc ggggcatgaa gctccgcaag
   110761 cggtgccgct tccaggtagg cttcatcacc actgacctga cgcttatagc gtgagtcaga
   110821 actacaggca gcgagtaata aaacaagcga aacacccgca acctttgcca ggcgcgactt
   110881 ttgaacagag taagccatca aatctcccta aactttacag caaaccggca tgcttaagcg
   110941 ccgctctgac cgtctcacga ccactgtcgg tgattggtgt cattggcagg cgcagcgtat
   111001 cggtcgccac aagacccagt tccttacatg cccatttcac cgggattgga ttgggttcga
   111061 caaatagttt gttgtgtaat ggcatcagac gctgattaat aacgcgtgcc tcggcaaaat
   111121 gcccttctgc tgccagtttg cacatccggg ccatatcacg cgctgcgacg ttagccgtaa
   111181 cggaaataac cccatgaccg cccaattgca tgaagtccag cgcgctcgca tcatcgccgc
   111241 tcagcagaac aaaatcatct gaaaccagct ctttgatctg gtttacacgc gttaagttcc
   111301 ctgttgcctc tttgattccg ataatatttt ttactttcgc cagacggccc accgtttccg
   111361 ggagcagatc gcagccagta cgggacggca cattatacag aatttgcggc aggtcagtat
   111421 gctcagcgat ggctttgaaa tgctgataca aaccttcttg cgacggacga ttgtagtaag
   111481 gggttaccgt caggcagccg acgataccac tgtcattgaa gcgctgcgtc aggctaatgg
   111541 cttccgcagt agcgttagcg ccggtcccgg caattaccgg aatgcgccca tcagccagat
   111601 ccagcgtcat catcaccaca tcagcatgtt cgtcatgatt taaggtagcg gactcgccag
   111661 tggtgccaac agaaacgatc gccgaagtac cgctggcgac atgataatca atcagttttt
   111721 tcaagctagc ccgacagaca ttaccttttt catccatcgg agtaacaatc gcgacaatac
   111781 ttcccgtgaa catgggccat cctctgtgca aacaagtgtc tcaatggtac gtttggtatg
   111841 gcattaaaag caagcagaca gaaccgttct gattgttgta tgcatgattt ttttatgctt
   111901 tccttaagaa caactcaccc cttaaaggaa taaccagttt gacactgtca tcgcaacatt
   111961 atctggtgat cactgcgttg ggtgccgatc gccctggaat tgtgaacacc atcacccgtc
   112021 atgtcagtag ttgcggctgt aatattgaag acagtcgcct ggcgatgctg ggagaagagt
   112081 tcacgtttat catgctgctt tccggttcat ggaatgccat cactctgatt gaatcaacgt
   112141 taccgttgaa aggtgccgaa ctggatcttt taatcgtgat gaagcgcacg acggcgcgtc
   112201 cgcgtccgcc aatgccagca tctgtctggg ttcaggtcga tgttgcagac tccccgcatt
   112261 taattgaacg cttcacagca cttttcgacg cgcatcatat gaacattgcg gagctggtgt
   112321 cgcgcacaca acctgctgaa aatgaacggg ctgcgcagtt gcatattcag ataaccgccc
   112381 acagccccgc atctgcggac gcagcaaata ttgagcaagc gttcaaagcc ctatgtacag
   112441 aactcaatgc acaaggcagt attaacgtcg tcaattattc ccaacatgat gaacaggatg
   112501 gagttaagta atgaatccac tgaaagccgg tgatatcgca ccgaaattta gcttgccgga
   112561 tcaagacgga gaacaagtta atttgaccga cttccaggga cagcgtgttc tggtttattt
   112621 ctacccgaaa gccatgaccc ccggctgtac cgtacaggcc tgcggcttac gcgataacat
   112681 ggatgagttg aaaaaagcgg gcgttgatgt gctgggtatc agcaccgata aacccgaaaa
   112741 actctcccgt tttgcggaaa aagagctgct taactttacg ctcctgtctg atgaggacca
   112801 ccaggtgtgc gaacaattcg gcgtctgggg tgaaaagtcc ttcatgggca aaacctacga
   112861 tggtattcat cgcatcagct tcctgattga cgctgatggc aaaatcgaac atgtctttga
   112921 cgacttcaaa accagcaatc accacgacgt tgtgctgaac tggctgaaag aacacgcctg
   112981 attactttgc tccattccgt gctggctgcg cttgcggcca gcatacctca cttctcgtga
   113041 tcaagatcac attctcgctt tcccctgcga cacgggtgtc gaatccattt tttgctgaac
   113101 gttaatgacc atcatttttg taccgttcag aatccagtta atacataact tattgaatag
   113161 attgagttaa tcagaatggc accctttatg caatatgaaa tgcaatgttt catatcattt
   113221 tcaaggagcc gacatgaacc gctttgtggt ggccgaacca ctgtggtgta caggatgtaa
   113281 tacctgtctc gctgcctgtt cggacgtgca taaaacgcaa ggtttacagc aacacccgcg
   113341 cctggccctg gcgaagacgt caacaatcac tgcccctgtc gtgtgtcatc actgtgagga
   113401 agccccttgc ctgcaggtct gcccggtcaa tgccatctct cagagggatg atgcgatcca
   113461 actcaacgaa agcctctgta ttggctgcaa gctttgcgcc gtggtctgcc catttggcgc
   113521 aatcagcgct tcaggaagcc gtccggtgaa tgcccatgcg caatatgttt ttcaggctga
   113581 aggctcactc aaagacggcg aagaaaacgt gccgccccaa catgcgttgc tgcgctggga
   113641 accgggtgtc cagaccgtcg cggtgaaatg cgacctgtgt gatttcttgc cagaaggtcc
   113701 ggcctgcgtt cgcgcttgcc cgaatcaggc gttacggctg atcaccgacg atagcctgca
   113761 acgtcagatg aaagaaaaac agcgccttgc cgcaagctgg tttgccaatg gcggggagga
   113821 tcccctttcc ctcactcagg agcaacacta atggatgccc tgcaattatt aacctggtcg
   113881 ctgatcctct atctgtttgc cagtctggct tcgctgtttt tactcggtct ggacagactg
   113941 gctattaagc tttccggcat cacatcgctg gtgggcggcg tgattggcat catcagcgga
   114001 attacgcaat tacatgctgg tgtaacttta gtcgcccatt ttgccacccc ttttgacttt
   114061 gccgatttaa ccctgcgaat ggatagcctc tcggcattta tggtgctggt tatctccttg
   114121 ctggtggtgg tttgttcgct ctattcattg acttatatgc gcgaatacga gggcaaaggc
   114181 gcggcggcga tgggcttctt tatgaatctt ttcatcgcat cgatggttgc cctgctggtg
   114241 atggacaacg ctttttggtt catcgtgctg tttgaaatga tgtcgctgtc ttcctggttt
   114301 ctggtcattg ccaggcagga taaaacgtcg atcaacgctg gcatgctcta cttttttatc
   114361 gcccacgccg gatcggtgct gattatgatc gccttcttgc tgatggggcg cgaaagcggc
   114421 agcctcgatt ttgccagttt cgcacgcttt cactttctcc ggggctggcg tcggcggtgt
   114481 ttctgctggc ctttttcggt tttggcgcga aagccgggat gatgccgttg cacagctggt
   114541 tgccgcgcgc tcaccctgcc gcaccatcgc acgcttcggc gttgatgtct ggcgtaatgg
   114601 tcaaaatcgg tattttcggc atcctgaaag tagcgatgga tctgctggcg caaacgggtt
   114661 tgcctctgtg gtggggcatt ctggtgatgg cgatcggcgc aatctccgcg ctcctgggcg
   114721 tgctatatgc gctggcggaa caggatatca aacggctgct ggcctggagt accgtcgaaa
   114781 acgtcggcat tattttgctg gcagtcggtg tggcgatgga cggtctgtca ctgcacgacc
   114841 cgctgctcac cgtggttgga ctgctcggcg cactgtttca tctgctcaac catgcgctgt
   114901 tcaaagggct gctatttctc ggcgcggacg cgattatttc gcgtttgcat acccacgaca
   114961 tggaaaaaat gggggcactg gcgaaacgga tgccgtggac agccgcagca tgcctgattg
   115021 gttgcctcgc gatatcagcc attcctccgc tgaatggttt tatcagcgaa tggtacacct
   115081 ggcagtcgct gttctcacta agtcgtgtgg aagccgtagc gctacaactt gcgggtccta
   115141 ttgctatggt aatgctggca gtcactggtg ggctggcagt aatgtgcttc gtcaaaatgt
   115201 acggtattac tttctgtggt gcgccgcgca gtacacacgc tgaagaggca caggaagtgc
   115261 caaatacgat gatcgtcgcc atgctactgc tcgcggcact ctgcgtatta attgcgctta
   115321 gtgccagttg gctggcaccg aagataatgc atattgccca tgcgtttacc aatacccctc
   115381 ccgtcactgt cgccagcgga atagcacttg tacccggcac gtttcataca cgggtcactc
   115441 cttcattact gttgctgtta ctactggcga tgcctttgct gccaggcctt tactggctgt
   115501 ggtgtcgttc gcgccgcgca gcgtttcgtc gcacaggaga tgcctaggca tgcggctacg
   115561 gctgggaaaa cgcgatggct ccgtcaggca atggcgtgat gcagccgctg cgtgtggtct
   115621 tctctgcgtt atttcgtcta cgacaacagc tcgaccctac gctgaggcta aataaaggtc
   115681 ttgcgcacgt caccgccagg gctcagagca cagaaccctt ctgggatgag cgggtgatcc
   115741 gccccatcgt gagcgccacc caacggctgg tcaaagaaat acagcatctg caaagcggcg
   115801 actttcgtct ctattgcctg tatgtggtcg ccgcactggt tgtgctgcta atcgctattg
   115861 ccgtctaagg aaatcaccat gagacaaact ctttgcgacg gatatctggt catttttgcg
   115921 ttagcacagg ccgtgattct gctgatgcta accccacttt ttacgggtat ttcccggcag
   115981 atacgcgcgc gtatgcactc ccgccgcggg ccggggatct ggcaggatta tcgcgatatc
   116041 cacaaactgt ttaaacgcca ggaagttgcg ccgacatctt caggtctgat gttccgcctg
   116101 atgccgtggg tattaatcag cagcatgctg gtgctggcga tggccttacc actatttatt
   116161 accgtttccc cttttgcggg cggcggcgat ctgctcaccc ttatctatct tcttgccctg
   116221 tttcgttttt tctttgctct ttccgggctg gataccggaa gtccgtttgc gggagtcggt
   116281 gccagtcgcg agttgacgct cggcattctg gtcgaaccaa tgcttattct ctcactgctg
   116341 gtattggcgc tgatagcagg ttccacgcat atcgagatga tcagcaatac gctggcgatg
   116401 ggctggaact cgccgctaac caccgtactg gcgttactgg cctgtggttt tgcctgcttc
   116461 attgagatgg gaaaaattcc ctttgatgtt gctgaagcag aacaggaatt acaggaaggc
   116521 ccgctgaccg aatattccgg tgccgggctg gcgctagcga aatgggggca ggggctgaaa
   116581 caggtcgtga tggcagcact gtttgtggcc ctgtttctgc cctttgggcg cgcgcaagaa
   116641 ctttctctcg cctgcctgct gacttcactt gtcgttacgc tgctcaaggt tttgctgatt
   116701 tttgtactgg cctcaatcgc agaaaacacg ctggcacgcg ggcgtttttt actcattcac
   116761 catgtgacct ggcttggctt cagccttgct gcgcttgcat gggtcttctg gttaaccggt
   116821 ctgtaaggag cactgacgga atatggaaaa tcttgctctg acgacgttaa tgctgccttt
   116881 tatcggcgca ctggtcgttt cgttttcgcc acaacgtcgg gccgccgaat ggggggtttt
   116941 gttcgccgcg ctgaccacgc tgtgcatgtt gtcgctgatc tccgcgtttt atcaggccga
   117001 taaagttgcc gtcacgttga cgttggtcaa cgtgggggat gtggcattgt ttggcctggt
   117061 cattgatcgc gtgagtacgc tgattctgtt tgtggtggtg tttctcggtt tgctggtcac
   117121 gatctactcc acgggttatc tgacggataa aaatcgcgaa cacccgcata acggcacgaa
   117181 tcgttattac gcatttttgc tggtgtttat cggcgcgatg gcgggactgg tactctcctc
   117241 gacgctgctc ggtcagttgt tgttttttga aattacgggc ggctgctcct gggcgttgat
   117301 cagttattac cagagcgata aagcgcagcg ttcagcacta aaagcgttgc ttatcaccca
   117361 tatcggttcg ttggggttgt atcttgccgc cgccacgctg tttttgcaga ccggaacgtt
   117421 tgtgcttagc gcgatgagcg agttacatgg cgacgcacgt tatctggttt atggcggcat
   117481 cctgtttgcc gcgtggggga aatcggccca gctaccgatg caagcgtggc taccggacgc
   117541 aatggaagcg ccaacaccga tcagcgacta tctccacgcc gcatcgatgg tgaaagtggg
   117601 cgtttacatt tttgcccgcg ccattatcga cggcggcaat atcccgcatg tgattggcgg
   117661 cgttggcatg gtcatggcac tggtcaccat tctttatggc tttctgatgt atttgccaca
   117721 gcaggatatg aagcggttgc tggcctggtc gaccatcact caacttggct ggatgttctt
   117781 cggcttgtcg ctctccatct tcggctcgcg gctggcgctg gagggtagca tcgcctacat
   117841 cgtcaaccac gcgttcgcta aaagcctgtt tttccttgta gcaggtgcgc tgagttacag
   117901 ctgcggcacg cgcttgttgc cgcgtctgcg tggcgtattg cacaccctgc cgttgccagg
   117961 cgtgggtttc tgcgtggcag cgctggcaat taccggtgtg ccgccgttca acggcttctt
   118021 cagtaaattc ccgctgtttg ccgccggttt tgcgttgtca gtggagtact ggatcctgct
   118081 gcccgccatg attctgctga tgattgaatc ggtcgccagt ttcgcctggt ttattcgctg
   118141 gtttggtcgc gtcgtgccgg gcaaaccgag cgaggccgtc gccgatgccg caccgctgcc
   118201 agggtcaatg cgcctggtgt tgattgtact gattgtgatg tcgctgattt ccagcgtaat
   118261 cgccgcgacc tggttgcagt aaggagatga tgaatgaccg gttctatgat cgtaaataat
   118321 ctggcgggac tgatgatgct gacatcgctg tttgtgatta gcgtcaaaag ctatcgcctg
   118381 tcatgcggat tttaggcctg ccagtcgctg gtgctggtgt ctattttcgc cactctctcg
   118441 tgcctgttcg ccgcagagca actgctgatc tggtccgcca gcgcctttat caccaaagtg
   118501 ctgctggtac cgttaatcat gacttacgct gcacgaaata ttccccagaa catcccggaa
   118561 aaagcgttat tcggtccggc aatgatggca ctgctcgcgg cgttaattgt cctgctttgc
   118621 gcatttgtcg ttcagcccgt gaagctaccg atggctaccg ggctgaaacc ggcgctggcg
   118681 gtagcgttag gtcattttct gcttggcctg ctgtgcattg tcagccagcg caatatcctg
   118741 cggcaaattt ttggttactg cctgatggaa aacggctccc atctggtgct ggcgcttctt
   118801 gcctggcgag caccggaact ggtggaaata ggtatcgcta ccgacgccat cttcgccgtc
   118861 attgtgatgg tgttactggc aagaaaaata tggcgtaccc acggcacgct ggacgtgaac
   118921 aacttgaccg cgctgaaggg ataatgagat gagttattct gtgatgttcg ctttactcct
   118981 gctcacgccg ctgctttttt cgctgctctg ttttgcctgc cggaaacggg ggctctctgc
   119041 gacttgtacg gtgactgtat tacatagctt agggatcaca ctgctgctga ttctggcact
   119101 ctgggtggtc caaactgccg ctgatgcagg agaaatattc gctgcgggac tgtggcttca
   119161 tattgatggt ctgggcggtt tgttcctcgc cattcttggt gtgattggct ttctcaccgg
   119221 tgtttactcg attggctaca tgcgtcatga agtggcacac ggcgagcttt cacccgttac
   119281 actgtgcgat tactacggtt tcttccatct gtttttgttc accatgctgc tggttgttac
   119341 cagcaataac ctgattgtga tgtgggcggc gatcgaagcc accaccttaa gctcggcgtt
   119401 tctggtaggc atttacggtc agcgttcatc gctggaatca gcatggaagt acatcattat
   119461 ttgtactgtt ggtgtcgctt ttggtctgtt cggtaccgtg ctggtatacg ccaacgccgc
   119521 cagcgttatg ccgcaggcag aaatggcgat attctggagc gaggttctta agcaatcgtc
   119581 cttgcttgac ccaacattaa tgctgttggc ctttgtgttt gtgctaattg gctttggtac
   119641 caaaaccggg ctatttccca tgcacgcctg gctgccggat gctcacagtg aagcgccgag
   119701 tccggtcagc gccctgctct ccgccgtatt gctgaactgc gcgctgttgg tgctgattcg
   119761 ctattacatc attatttgcc aggccatcgg cagcgatttc cccaaccggt tgttgctcat
   119821 cttcggcatg ttgtcggttg ccgtggcggc atttttcatt ctggtacagc gggacattaa
   119881 gcgtctgctg gcgtactcca gcgtggagaa catggggctg gtcgcggtgg cgctaggcat
   119941 tggcgggccg ctgggaattt ttgccgcgct gctgcacacc ttaaaccaca gtctggcaaa
   120001 aacgctgctg ttctgcggtt ccggcaatgt actgctcaag tacggcacgc gcgatctcaa
   120061 cgtcgtctgt gggatgctca aaatcatgcc atttaccgcc gtgctgtttg gcggcggtgc
   120121 gctggcgctg gcagggatgc cgcccttcaa catttttctt agcgaattta tgaccgttac
   120181 cgcaggactg gcacgtaatc acctgctgat tatcgtcctg ctgttattgc tgttaacgct
   120241 ggtgctcgcg ggcctggtac ggatggctgc gcgggtgtta atggcgaaac cgccgcaggc
   120301 cgttaaccgg ggtgatctcg gctggttgac cacctcgcca atggtgattc tgctagtcat
   120361 gatgctggcg atgggaacgc atattccaca acctgtcatc aggatcctgg cgggcgcttc
   120421 cactatagtc ctctcaggga cgcacgacct gcctgcacaa cgtagcacct ggcatgattt
   120481 tttgccttca ggcaccgcat ctgtttcgga gaaacacagt gaacgttaat tcatcgtcaa
   120541 atcgtggcga agcgattctc gccgccctga aaacgcagtt ccccggcgcg gtgctggatg
   120601 aagagcgaca aacgcctgaa caggtcacca ttacggtgaa aatcaatctg ctgcctgacg
   120661 ttgtacatta tctttattat caacatgatg gctggcttcc agtcctgttt ggcaacgacg
   120721 agcggacact taacggtcat tacgcggttt attatgccct ttcaatggaa ggggccgaaa
   120781 aatgctggat cgtggtgaag gcactggtcg atgccgacag tcgggagttt ccgtcagtca
   120841 caccgcgcgt ccctgccgcg gtctggggcg agcgagaaat tcgcgatatg tacgggctga
   120901 ttccggttgg cctgccggat cagcgtcgcc tggtgttgcc cgatgactgg ccggaagata
   120961 tgcatccgct gcgcaaagat gcgatggatt atcgactgcg ccctgaaccg acgacagatt
   121021 ccgaaacgta tgcgtttatc aatgagggca acagcgatgc gcgggtgatc cctgtcggcc
   121081 cgctgcatat cacctcctat gaaccgggtc acttccgctt gtttgtggat ggcgagcaaa
   121141 ttgtcgatgc tgattaccgc ctgttttatg tccatcgcgg catggagaaa ctggcagaaa
   121201 cgcggatggg ctacaacgaa gtgaccttct tatcggaccg cgtgtgtggg atttgcggtt
   121261 ttgcccacag tgtggcctat accaactcgg ttgaaaatgc actggggatt gaggtgccgc
   121321 aacgagcaca tactattcgc tcgattctgc tggaagtcga gcggctacac agtcatttgc
   121381 tcaaccttgg cctctcctgc cattttgttg gttttgatac cggctttatg caatttttcc
   121441 gcgtgcggga aaagtcgatg acgatggcgg aattgctgac cgggtcgcgt aaaacctacg
   121501 gtctgaatct gattggtggt gttcgccgcg atattctcaa agagcaacgt ctgcaaacgc
   121561 tgaaactggt gcgcgagatg cgcgccgacg tgtcggagct ggtagagatg ctgcttgcta
   121621 cgccgaatat ggaacaacgc actcagggca ttggcattct cgaccgacaa atcgcccgtg
   121681 attatagccc tgtagggccg ctgatccgcg cagtggtttt gcccgtgatt tgcgctttga
   121741 tcacccctac gccgactacg gcaatattcc aaaaacactg tttaccttta ccggcggcga
   121801 tgttttctcc cgcgtgatgg tccgtgtcaa agagacgttt gattcgctgg caatgctgga
   121861 atttgccctc gacaacatgc cggatacccc actgctgacc gaaggcttta gctataaacc
   121921 tcacgcattc gcgctgggct ttgttgaagc gccacgcggt gaagacgtgc actggagcat
   121981 gctcggtgat aaccaaaaat tgttccgctg gcgctgtcgt gccgccacct acgccaactg
   122041 gccggtgttg cgttacatgc tgcgcggcaa taccgtttct gacgcaccgc tgattatcgg
   122101 tagccttgat ccctgctact cctgtaccga ccgtgtgacg ctggtagatg tgcgcaagcg
   122161 ccagtcaaaa accgtgctgt ataaagagat cgaacgctac ggcattgatc gtaaccgttc
   122221 gccgctgaag taaggacaga agatgctgaa gttactgaaa actattatgc gcgccggaac
   122281 cccgacggtg aaatatccct tcgcgccact ggaggtcagc ccaggctttc gcgggaaacc
   122341 ggacctgatg cccagccaat gtattgcctg cggtgcctgc gcctgtgctt gtccggcaaa
   122401 tgcgctgact atccagaccg acgaccagca aaattcgcgc acctggcagc tctatctggg
   122461 gcgttgtatt tactgcggac gttgtgaaga agtgtgcccg accagagcca tccagcttac
   122521 caataacttt gaactgaccg tcacccataa agccgatctc tatacccacg cgacgttcca
   122581 tctacaacgt tgcagccgtt gcgaacgccc gtttgccccg caaaaaaccg tcgcactggc
   122641 tgctgaattg ttggcacagc aacaaaatgc tccacaaaac cgcgaaatgt tatgggcgca
   122701 agcgagcgtc tgcccggagt gcaaacaacg cgcgacgctg atcaacgacg atacagatgt
   122761 accgctggtg gctaaggagc agctatgagt ccagtgctta cgcaatatgt cagccagccc
   122821 atcacgctgg acgagcaaac gcaaaagatg aagcggcatt tgctacagga tatccgtcgc
   122881 tcggcttacg tttatcgcat cgattgcggc ggctgcaacg cctgtgaaat cgaaattttt
   122941 gctgccatca cccccgtatt cgacgcagaa cgttttggca ttaaggttgt ttcatcaccg
   123001 cgtcacgccg atattttgtt atttactggc gcagtcaccc gggcgatgcg tatgcctgca
   123061 cttcgggcgt atgagtctgc ccccgatcat aaaatctgtg tttcctacgg cgcgtgcggt
   123121 gtcggcggcg gtattttcca cgatctctac agcgtctggg gcggcagcga caccattgtc
   123181 cccattgatg tttggatccc cggctgcccg ccaacaccgg ccgccaccat tcacggtttc
   123241 gccgtggcgc tcggtttgct gcaacagaag attcacgcgg tggattatcg cgatcccacc
   123301 ggggtgacta tgcaaccgtt gtggccgcag atcccgccat cacagcgtat cgccattgag
   123361 cgagaagcgc ggcggctggc gggctatcgt caggggcgag aaatttgcga tcggctcctg
   123421 cgccatttaa gcgacgatcc tacaggaaat cgggttaaca cctggttgcg cgatgccgac
   123481 gatccacgtc tcaatagtat cgttcagcaa ctctttcgcg tactccgggg gttacatgac
   123541 tgaagagtgc ggggaaattg ttttctggac gctgcgaaaa aagtttgtcg ccagtagcga
   123601 cgagatgccg gaacacagct ctcaggtaat gtattactcg ctggctatcg gtcatcacgt
   123661 tggcgtgatt gattgtctga atgtcgcctt ccgctgccca ctgacggaat acgaagattg
   123721 gcttgcactg gtcgaagagg agcaagcccg acgtaagatg ctgggggtga tgacttttgg
   123781 tgagattgtt attgacgcca gccacaccgc cctgttgacc cgggcattcg cgccactggc
   123841 agatgacgcg acgtctgtgt ggcaagcgcg tagcattcaa ttcattcatc tgttggatga
   123901 aattgtgctg gaaccggcta tctatctgat ggccagaaaa attgcgtgag aaggatttct
   123961 cattaataag gactgttgat ggctatgtca gacgaagcga tgtttgcccc gccgcaagga
   124021 ataacaattg aagcggtaaa cggaatgctc gcggagcggt tagcgcagaa acacggcaag
   124081 gcgtctttat tacgcgcctt catcccgctg ccgccgccgt tcagcccggt acaacttatt
   124141 gaactgcatg ttctcaaaag caacttctat taccgctacc atgatgatgg cagcgatgtg
   124201 acggcaacaa cagagtatca gggcgagatg gtcgattatt cgcgtcacgc cgtccttctc
   124261 ggcagtagtt gaatggcgga gctacgcttt attcgtaccc acggcagccg ttttactccc
   124321 caggattgca cactgtttaa ctggctggca cgcattatca ccccggttct gcaatcatgg
   124381 ctcaatgatg aagaacagca ggtggcgctg cgtttgctgg agaaagatcg cgatcatcat
   124441 cgggtactgg tggatatcac taatgcagtg ctgtcacatc ttgatctcga cgatctgatc
   124501 gctgacgtcg ctcgtgagat ccatcatttt ttcggtctgg cttcagtcag tatggtactg
   124561 ggcgatcatc gaaagaacga gaagttcagc ctgtggtgca gcgatctttc tgcctcacat
   124621 tgtgcgtgtc tgccacacaa tatgcctggc gacagtgtat tgctgacaca aacgctacaa
   124681 acccgacaac cgaccttgac gcaccgtgca gatgatctgt ttctctggca acgcgacccg
   124741 ttattactct tacttgcatc taacggctgc gaatctgcgc tccttatacc gcttaccttt
   124801 ggcaaccata caccgggtgc attgttgctg gcgcatacct cttccactct ctttagtgag
   124861 gaaaactgcc agctactaca acacatagcc gatcgcatcg ctattgccgt tggcaatgcc
   124921 gatgcctgcc gtagaatgac cgatttgcag gaaagtttgc agcaagaaaa ccaccagctt
   124981 agcgagcagc tcctttcgaa tctgggcgtc ggtgacatta tctatcaaag ccaggcaatg
   125041 gaagacctgc tccagcaggt agatattgtg gcgaagagcg acagtacggt gttgatttgc
   125101 ggtgaaaccg gaaccggcaa agaggtgatc gccagagcga tccatcaact tagcccgcga
   125161 cacgacaagc cgctggtcaa aatcaactgc gctgccatcc ccgccagtct tctggaaagt
   125221 gagttattcg gtcatgacaa aggggcgttt actggtgcga ttaataccca tcgtggtcgt
   125281 tttgaaattg ccgatggcgg cacgttgttt ctcgatgaaa ttggcgatct gccgttagaa
   125341 cttcagccta aactgctgcg cgtattgcag gaacgggaga ttgagcgtct cggcgggagt
   125401 agaacgatcc cggtaaatgt cagagtcatt gccgccacca accgtgattt gtggcaaatg
   125461 gttgaagatc gccagtttcg cagcgatctc ttttatcgcc tgaatgtctt cccactggaa
   125521 ttgcctccgc tgcgcgaccg tccggaagat atccctcttt tagcaaagca tttcacgcaa
   125581 aaaatggcgc gccatatgaa tcgttcaatt gacgccatcc cgaccgaggc gctacgccag
   125641 ttgatgtcgt gggactggcc gggcaacgtg cgcgagctgg aaaacgtgat tgagcgagcg
   125701 gtactgctga ctcgcggtaa cagtctgaat ttacatctta atgtccgaca aagccgttta
   125761 ctgccgacgc taaatgaaga ttcagcgctt cgcagttcaa tggcgcagtt gctgcacccg
   125821 acgacgccag agaatgacga agaagaacgt cagcgcattg ttcaggtatt gcgagaaacc
   125881 aatggcattg ttgccgggcc ccgtggcgcg gcgacacgat tagggatgaa gcgcaccacg
   125941 ctgctgtcac gaatgcagcg tctgggaatc tcggttcgcg aggtgttgta atctgctttt
   126001 gcaggagtat gcatgagaaa caaactctct ttcgacttgc agttgagcgc cagaaaagcg
   126061 gcaatcgctg aacggattgc cgcccataaa attgcccgca gtaaagtgtc ggtcttttta
   126121 atggcgatgt ccgctggcgt gtttatggcg atcggattta ctttttacct ttccgttatc
   126181 gccgatgccc cgtcttcata ggcattaacc catctggtgg gcggcctttg ctttacactt
   126241 ggctttattt tgctggcggt ttgcggcacc agcctgttca cctcgtcggt aatgacggta
   126301 atggcaaaaa gtcggggcgt tattagttgg cgaacttggc tgattaacgc acttctggtg
   126361 gcctgcggta atctggcagg tattgcctgt ttcagtttgt taatctggtt ttccgggctg
   126421 gtgatgagtg aaaacgcgat gtggggagtc gcgattttac actgcgccga gggcaaaatg
   126481 catcatacat ttactgaatc tgtcagcctc ggcattatgt gcaatctgat ggtttgcctg
   126541 gcgctgtgga tgagttattg cggacgttcg ttatgcgaca aaatcgtcgc catgattttg
   126601 tccatcaccc tgtttgtcgc cagtggcttt gagcactgta tcgccaattt gtttgtgatt
   126661 ccgttcgcca ttgccattcg ccatttcgcc cctacctcct tctggcaact ggcgcacagt
   126721 agcgcagaca attttccggc actgacggtc agccatttta ttaccgccaa tctgctcccg
   126781 gtgatgctgg gtaatattat cggcggtgca gtgctggtga gtatttgtta tcgggctatt
   126841 tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac agttcgtcgg gctgaagatt
   126901 attcttgcgc gatttgccca tcgggccagg cgtggatcac ggctttgatc agcgtcgcca
   126961 atggaatggc gaagaatacg ccccagaatc cccacaaacc accgaagatc accaccgata
   127021 aaataatcac cagcggatgc aggttaaccg cttcggagaa caacaccggt actagcaggt
   127081 tgccgtccag cgcctgaata atcagataca ccgcgaagca gctccagaat tcagtacctg
   127141 cgccaaactg gaatagcgcc acgccaacca ccggaatggt caccacaaat gcgccaatgt
   127201 acggaataag aaccgagaag ccgaccagca ctgccagcag cagcgaatag ttcagcccga
   127261 agagcaagaa ccccagccag gtggcgatcc ccaccacgat catctccagc actttgccgc
   127321 ggatatagtt ggtgatttgt tgattcatct ccttccacac ctgtcctgcc agcccacggt
   127381 tacgcggcag cacccggcga acggcgttca gcatctgctc tttgtctttc agcaggaaga
   127441 agaccatcaa tggcaccagc accagataga cggctatggt cagcaaaccg accagcgagg
   127501 cgagggaaat tttcaccacc gaatcgccca tggtcagcat ccgactgcgc atattttcgg
   127561 ccattgcatc aataatgccc gcgtccatta acaccggata gcggcgcggc aacgtggcgg
   127621 caaagtcaga aagcttattg agcatccccg gcatatcgcg gattaagtag atgccctgtt
   127681 gccaggcgat aggcagtacc acgaacgcca tcagtagcaa tataccgaca aaaaccacca
   127741 atacaatcga cgtcgcccag cggcgggagc agccaataga ttgcaggcgc acggttggcc
   127801 attccagcaa ataagccagc acaatagcca ccagcaacgg agcaagcagg ccactaaaga
   127861 aaaagataat gccaaatccg gcaactaaaa taaccagcaa ggcaatcgct tccgggtcgc
   127921 taaaacggcg gcgataccat tgcatcaaca tttcgagcat aaagaccttc cctgaacctc
   127981 aagagcggga ttgcgatccg caattgtatc gaaatgtcac aaaaaagact tcgcttttta
   128041 tgacggattc aggaaactga aaagtcattt gagtgggcta atcttcgccg ttacactcaa
   128101 aggcggcgag gtgggaacga tatttcacag tatcggtcaa atgactatct tcagaaatac
   128161 aggatagagg ttatgttcag gcagttgaaa aaaaacctgg ttgcaaccct cattgctgct
   128221 atgaccattg gtcaggtagc cccggcgttt gccgacagcg cagacacctt gccggatatg
   128281 ggaacctccg caggaagcac gctttccatt ggtcaggaaa tgcagatggg cgactattat
   128341 gtccgccagc tacgcggcag cgcgccgtta attaatgacc cgctgttaac gcaatatatt
   128401 aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg ttaagacacc gtttcatttt
   128461 tttctgatca acaacgacga aattaacgcc tttgctttct ttggcggcaa cgtggtgctg
   128521 cactctgccc tgttccgtta ttccgataac gaaagtcaac tggcttcagt tatggcgcac
   128581 gaaatctccc acgtcaccca acgtcacctg gcgcgagcga tggaagatca acaacgcagc
   128641 gcgccgctga cctgggtcgg cgcgttaggt tctattttac tggcgatggc cagtccgcag
   128701 gcggggatgg cggcgctgac cggtacactg gcgggaacgc gccaggggat gatcagtttc
   128761 acccagcaaa atgaacagga agcggaccgc attggtattc aggtgctgca acgctcggga
   128821 ttcgatccac aggcgatgcc gaccttcctg gaaaaattgc tcgatcaggc gcgttactcc
   128881 tcgcgcccgc cggaaatttt attgactcac ccgttgccgg aaagtcgtct ggcagatgcc
   128941 cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt cgtcggaaga tttctatctg
   129001 gcgaaagcgc gcacactggg gatgtataat tccggacgta accagctcac cagtgatttg
   129061 ctggatgaat gggcgaaagg aaacgttctt cagcaacgag cggcgcaata tggtcgtgct
   129121 ttacaggcga tggaagccaa taaatacgac gaggcgcgta aaacgctgca accgttactg
   129181 gcggcagaac ctggcaacgc atggtatctc gatctggcta ccgatatcga tcttgagcaa
   129241 aacaaagcca atgaggcaat caatcgcctg aaaaatgccc gcgatttacg caccaatccg
   129301 gtgttgcagc tcaacctggc gaacgcttat ctgcaaggcg gtcaaccaca agaagcggcc
   129361 aatattctga atcgctacac ctttaataat aaagatgaca gcaacggctg ggatttgctg
   129421 gcacaggcgg aagccgcgct aaataaccgc gatcaggagc tggctgcgcg agcagaaggt
   129481 tatgcgctcg ccggacgact cgatcaggcc atttcgctgt tgagtagcgc cagttcgcag
   129541 gtgaaattag gcagcctgca acaagcgcgt tacgatgcgc gcatcgacca gttgcgccag
   129601 ctgcaggaac gctttaagcc ttataccaag atgtaattcg cattaaagga agaacaatga
   129661 ccaaacaggt aaaaatctac cataacccac gctgttcaaa gagccgggaa acgctgaacc
   129721 tgctgaaaga aaacggcgtg gagccagaag tggtgctcta ccttgagaca cccgccgatg
   129781 cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa tagcgcccgt gaattgatgc
   129841 gccagaaaga ggatctttat aaagaactaa acctggctga cagttcgctt agcgaagagg
   129901 cgctgattca ggcaatggtg gataatccga agctgatgga gcgcccgatt atggtcgcga
   129961 atggcaaagc gcggattggt cgtccaccgg agcaggtact ggaaatcgtg ggttaagatg
   130021 tgctccatcg ctagttgaag cacattgccg gatgcgacgc ttgccacgtc ttatccggcc
   130081 tacgaatact atggattaac ttcgcgtagg tcaggcgttc gcgccgcatc cgacaataaa
   130141 caccttatct acaacttcag aatttctttc acaaacggaa tggtcagctt acgttgcgcg
   130201 gtaatcgacg cacgatccaa ctgatccaac gtcataaata gcgtgcgcat ttctctgtcg
   130261 agccgcttca gcaagaaacg ccccacatct tccggcagtt caaaaccacg caaacgcgcg
   130321 cgtaactgta gcgcctgcaa cttatcttca tcagaaagtg gctgcaattt gtagatctgc
   130381 ccccagtcga gacgcgacgc gagatccggt aatcccagat tcaactgccg cggtggacga
   130441 tcgccggtga tcaacaaccg tgttttgccc gattccagaa ttcgattgta gagatcgaaa
   130501 atcgccatct cccacaactc atcgcctgca atacactcaa tgttgtcgat acagaccagc
   130561 gacaaatgct ccataccgtc gagcacttcc ggaacaaacc aggtgcgttt atccagcggg
   130621 acatagccca ccgcatcgcc acgctgcgac aattccgcgc aagccgcgtg cagcagatgg
   130681 ctgcgccccg cgccttcgcg tgcccagaga tagatgtaac cgctatgttc ctgacgcagc
   130741 acgttttgca gcgcggccag taaagaggag ttatcccccg gccagaaact tgcaaaggtt
   130801 tcgtcgtcag gaagataaag tggcaaagag agctgtgccg gtgtgttcag agatacctca
   130861 accaggattt cacaaaatcg cgagaagttt accacagaat cccatgatgt ttgaaccggg
   130921 cagcaacact gcccggtcgg tacattattt gtctgttata tccgcgtctt ctgcgtccag
   130981 caccacttct tcagggcgca gcacactgat cagtttgaaa atcaaactta agccgatacc
   131041 gacgatggtc gccagcgcca taccttttaa ctccgccgcg ccgatgttta ccttcgcgcc
   131101 actgacgccg atgatcaaaa tcacggaagt caggatcagg ttctgtgctt tgttgtaatc
   131161 cactttcgat tcgatcaaaa cacgaatacc ggaagcacca atgacaccat aaagcagcag
   131221 cgaaacgccg cccatcaccg gcaatgggat catctggata gcggcagcca gtttaccgac
   131281 gcaggaaagc aggatagcga aaatcgccgc cccgccgata acccaggtac tgtaaacacg
   131341 ggtgatcgcc atcacgccaa tgttttctcc gtaagtggta tttggcgtag agccaaagaa
   131401 gccggagatc acggtcgaca ggccgttagc aaacatcgaa cggtgcagac ctggatcgcg
   131461 cagcagatct tttttgacga tattagccgt tactaccagg tgccctacgt gttcggcaat
   131521 aaccactaac gccgctggca gaatagtcag aatggcaaac cactcgaagc gcggcgtata
   131581 gagggttggc agtgcaaacc agtgagcatt aataatcggc gtggtatcga caattcccat
   131641 tgcgaaagag agcgcgtacc ccaccagcac gccaattaaa atcgggataa ttgccaggaa
   131701 accacgaaac agcacggaac ctaaaaccgt gaccgccagg gtggtaatag agatgatgat
   131761 ggttttggag tttggcgttt gcccttcagc cgggagtaaa cccgccatac cggcagctac
   131821 gcccgccagc tccagaccga tgacggcaac gattgcgccc attgccgcag gtggaaacag
   131881 cacgtccaac cagccggtcc ccgctttctt cacgataaaa gaaaccaggc agaacagcac
   131941 gccgcacata ataaagccgc ccagcgcgac ttcataccct aacggcaaca gtaacaatac
   132001 cggtgaaata aaggcaaagc tggaaccaag ataagccgga attttccctt tacagatgaa
   132061 gagatacagc agcgttccaa taccgttaaa taacagtaca gtcgccgggt taatatgaaa
   132121 taagacgggc accaggacgg ttgcaccaaa catggcgaac aaatgttgca aactaagcgg
   132181 gattgtctgt aaaagtggcg gtctttcact caccccgata gcacggcgcg tcatagtatt
   132241 atcctctgta ttatgtgtta taggcgcttt actcaaaaaa aagccgactc ttaaagtcgg
   132301 ctttaattat ttttattctt tatttcgtac caaagatttt gtcaccggca tcgccgaggc
   132361 ccggaataat gtatccgtgc tcgttcagtc cctggtcaat cgatgcagta tacagttcga
   132421 cgtccgggtg cgctttttcc agcgcagcga taccttctgg cgcagcgacc agcaccagaa
   132481 ctttgatgct gctgcagccc gcttttttca gcaggtcgat ggtcgcgata acggaaccac
   132541 cggttgccag cattgggtca acgatcagcg ccatacgctc atcgatgtta gaaaccagtt
   132601 tctggaagta cggtaccggc tccagcgttt cttcattacg gtacataccg acaacgctga
   132661 tgcgcgcgct cggaacgttt tccagcacac cgtccatcat accaagaccc gcacgcagaa
   132721 ttggcacaac ggtaattttc ttacctttga tctggtcgat ttctaccggg ccgttccagc
   132781 cttcgatagt tactttttcc gtttcgaggt cggcggtcgc ttcgtaagtc agcaggctac
   132841 ccacttcgga agcgagttcg cgaaagcgct tggtgctgat atcttgctca cgcatcagtc
   132901 ccagcttgtg tttgacgagt gggtgtttga cttccacgat cttcatactc tttctccttt
   132961 gaggtgcagc cacaaaaaaa aatcgacgga ttatacctcc tttcttcaag gcggcaatat
   133021 tattttcgtt gactttagtc aaaatgataa cagtttgaga taaagttatt ttatattcaa
   133081 atggttatga aagaagatta ttccatccga aaactaacct ttgccctggc acaagtcttc
   133141 tttcgccgcg cgcccgggga aaagacgtgc aaaaaggttg tgtaaaacag tctcgcaaac
   133201 gtttgctttc cctgttagaa ttgcgccgaa ttttattttt ctaccgcaag taacgcgtgg
   133261 ggacccaagc agtgaccgat aaaacctctc ttagctacaa agatgccggt gttgatattg
   133321 acgcgggtaa tgctctggtt ggaagaatca aaggcgtagt gaagaaaacg cgtcgtccgg
   133381 aagtgatggg cggtctgggc ggcttcggtg cgctgtgtgc attgccgcaa aaatatcgtg
   133441 aacccgtgct ggtttccggc actgacggcg taggtaccaa gctgcgtctg gcaatggact
   133501 taaaacgtca cgacaccatt ggtattgatc tggtcgccat gtgcgttaat gacctggtgg
   133561 tgcaaggtgc ggaaccgctg tttttcctcg actattacgc aaccggaaaa ctggatgttg
   133621 ataccgcttc agcagtgatc agcggcatcg cggaaggttg tctgcaatcg ggctgttctc
   133681 tggtgggtgg cgaaacggca gaaatgccgg ggatgtatca cggtgaaaat tacgatgtcg
   133741 cgggtttctg cgtgggcgtg gtagaaaaat cagaaatcat cgacggctct aaagtcagcg
   133801 acggcgatgt gctgattgca ctcggttcca gcggtccgca ctcgaacggc tattcgctgg
   133861 tgcgcaaaat tcttgaagtc agcggttgtg atccgcaaac caccgaactt gatggtaagc
   133921 cattagccga tcatctgctg gcaccgaccc gcatttacgt gaagtcagtg ctggagttga
   133981 ttgaaaaggt cgatgtgcat gccattgcgc acctgaccgg cggcggcttc tgggaaaaca
   134041 ttccgcgcgt attgccagat aatacccagg cagtgattga tgaatcttcc tggcagtggc
   134101 cggaagtgtt caactggctg caaacggcag gtaacgttga gcgccatgaa atgtatcgca
   134161 ccttcaactg cggcgtcggg atgattatcg ccctgcctgc tccggaagtg gacaaagccc
   134221 tcgccctgct aaatgccaac ggtgaaaacg cgtggaaaat cggtatcatc aaagcctctg
   134281 attccgaaca acgcgtggtt atcgaataat gaatattgtg gtgcttattt ccggcaacgg
   134341 aagtaattta caggcaatta ttgacgcctg taaaaccaac aaaattaaag gcaccgtacg
   134401 ggcagttttc agcaataagg ccgacgcgtt cggccttgaa cgcgcccgcc aggcgggtat
   134461 tgcaacgcat acgctcatcg ccagcgcgtt tgacagtcgt gaagcctatg accgggagtt
   134521 gattcatgaa atcgacatgt acgcacccga tgtggtcgtg ctggctggtt ttatgcgcat
   134581 tctcagcccg gcgtttgtct cccactatgc cgggcgtttg ctgaacattc acccttctct
   134641 gctgccgaaa tatcccggat tacacaccca tcgtcaggcg ctggaaaatg gcgatgaaga
   134701 gcacggtaca tcggtgcatt tcgtcaccga tgaactggac ggtggcccgg ttattttaca
   134761 ggcgaaagtc ccggtatttg ctggtgatac ggaagatgac atcaccgccc gcgtgcaaac
   134821 ccaggaacac gctatttatc cactggtgat tagctggttt gccgatggtc gtctgaaaat
   134881 gcacgaaaac gccgcgtggc tggatggtca acgtctgccg ccgcagagct acgctgccga
   134941 cgagtaatgc ccccgtagtt aaagcgccag ctctgccgct ggcgtttttc aattcacctg
   135001 taaatcgcaa gctccagcag tttttttccc ccttttctgg catagttgga catctgccaa
   135061 tattgctcgc cataatatcc aggcagtgtc ccgtgaataa aacggagtaa aagtggtaat
   135121 gggtcaggaa aagctataca tcgaaaaaga gctcagttgg ttatcgttca atgaacgcgt
   135181 gcttcaggaa gcggcggaca aatctaaccc gctgattgaa aggatgcgtt tcctggggat
   135241 ctattccaat aaccttgatg agttctataa agtccgcttc gctgaactga agcgacgcat
   135301 cattattagc gaagaacaag gctccaacgc tcattcccgc catttactgg gcaaaattca
   135361 gtcccgggtg ctgaaagccg atcaggaatt cgacggcctc tacaacgagc tattgctgga
   135421 gatggcgcgc aaccagatct tcctgattaa tgaacgccag ctctccgtca atcaacaaaa
   135481 ctggctgcgt cattatttta agcagtatct gcgtcagcac attacgccga ttttaatcaa
   135541 tcctgacact gacttagtgc agttcctgaa agatgattac acctatctgg cggtggaaat
   135601 tatccgtagc gatagcatcc gttacgcgct gctggagatc ccatcagata aagtgccgcg
   135661 ctttgtgaat ttaccgccag aagcgccgcg tcgacgcaag ccgatgattc ttctggataa
   135721 cattctgcgt tactgccttg atgatatttt caaaggcttc tttgattatg acgcgctgaa
   135781 tgcctattca atgaagatga cccgcgatgc cgaatacgat ttagtgcatg agatggaagc
   135841 cagcctgatg gagttgatgt cttccagtct caagcagcgt ttaactgctg agccggtgcg
   135901 ttttgtttat cagcgcgata tgcccaatgc gctggttgaa gtgttacgcg aaaaactgac
   135961 tatttcccgc tacgactcca tcgtccccgg cggtcgttat cataatttta aagactttat
   136021 taattttccc aatgtcggca aagccaatct ggtgaacaaa ccactgccgc gtttacgcca
   136081 tatttggttt gataaagccc agttccgcaa tggttttgat gccattcgcg aacgcgatgt
   136141 gttgctctat tatccttatc acacctttgg gcatgtgctg gaactgctgc gtcaggcttc
   136201 gttcgacccg agcgtactgg cgattaaaat taacatttac cgcgtggcga aagattcacg
   136261 catcatcgac tcgatgatcc acgccgcaca taacggtaag aaagtcactg tggtggttga
   136321 gttacaggcg cgtttcgacg aagaagccaa cattcactgg gcgaagcgcc tgaccgaagc
   136381 aggcgtgcac gttatcttct ctgcgccggg gctgaaaatt cacgccaaac tgttcctgat
   136441 ttcacgtaaa gaaaacggtg aagtggtccg ttacgcacac atcgggaccg ggaactttaa
   136501 cgaaaaaacc gcgcgtcttt atactgacta ttcgttgctg accgcagatg cgcgtatcac
   136561 caacgaagta cggcgggtat ttaactttat tgaaaaccca taccgcccgg tgacatttga
   136621 ttatttaatg gtgtcaccgc aaaactctcg ccgtctgtta tatgaaatgg tagaccgcga
   136681 aatcgccaac gcgcagcaag ggctgcccag tggtatcacc ctgaagctaa ataaccttgt
   136741 cgataaaggc ctggttgatc gtctgtatgc ggcctccagc tccggcgtac cggttaatct
   136801 gctggttcgc ggaatgtgtt cgctgatccc caatctggaa ggcattagcg acaacattcg
   136861 tgccatcagt attgttgacc gttaccttga acatgaccgg gtttatattt ttgaaaatgg
   136921 cggcgataaa aaggtctacc tttcttccgc cgactggatg acgcgcaata ttgattatcg
   136981 tattgaagtg gcgacaccgc tgctcgatcc gcgcctgaag cagcgggtgc tggacatcat
   137041 cgacatattg ttcagcgata cggtcaaagc acgttatatc gataaagaac tcagtaatcg
   137101 ctacgttccc cgcggcaatc gccgcaaagt acgggcgcag ttggcgattt acgactacat
   137161 caaatcactc gaacaacctg aataacccta tgccaataca cgataaatcc cctcgtccgc
   137221 aggagtttgc tgcggtcgat cttggttcaa acagttttca catggtcata gcccgtgtgg
   137281 tagatggtgc catgcagatt attggccgcc tgaaacagcg ggtgcatctg gcggacggcc
   137341 tggggccaga taatatgttg agtgaagagg caatgacgcg cggtttgaac tgtctgtcgc
   137401 tgtttgccga acggctacaa gggttttctc ctgccagcgt ctgtatagtc ggcacccata
   137461 cgctgcgtca ggcgctgaac gccactgact ttctgaaacg cgcggaaaag gtcattccct
   137521 acccgattga aattatttcc ggtaatgaag aagcccgtct gatttttatg ggcgtggaac
   137581 atacccaacc ggaaaaaggt cgcaaactgg ttattgatat tggcggcgga tctacggaac
   137641 tggtgattgg tgaaaatttc gaacctattc tcgttgaaag ccgccggatg ggttgtgtca
   137701 gctttgccca gctttatttt cctggcgggg tcatcaataa agagaatttt cagcgcgctc
   137761 gcatggcggc agcacaaaaa ctggaaactt taacctggca attccgtatt cagggctgga
   137821 acgttgcaat gggcgcttcc ggtaccataa aagccgccca tgaagtgtta atggaaatgg
   137881 gcgagaaaga cgggataatt accccggaac gtctggaaaa actggtaaaa gaaattttac
   137941 gtcaccgtaa tttcgcatcg ctgagtttac cgggtctttc cgaagagcgg aaaacagtct
   138001 tcgttcctgg actggcgatt ttatgtggtg tgtttgatgc tttagccatc cgtgaactgc
   138061 gcctttctga cggggcgtta cgcgaaggcg tactgtatga aatggaagga cgtttccgtc
   138121 atcaggatgt gcgtagtcgc accgccagca gcctcgccaa ccagtatcac atagacagcg
   138181 aacaggcccg acgagtgctg gataccacta tgcaaatgta cgaacagtgg cgggaacagc
   138241 aaccgaagct ggcgcatccg caactggagg cgctactgcg atgggccgcc atgctgcatg
   138301 aggtcgggtt gaatatcaac cacagcggtt tgcatcgcca ctccgcttat attctgcaaa
   138361 acagtgactt gccgggtttt aatcaggaac agcagctgat gatggcgaca ctgctgcgct
   138421 atcaccgtaa agcgattaag ctcgacgatc tgccgcgctt taccttgttt aagaagaaac
   138481 agttcctgcc actgatacag ctattgcgcc ttggcgtatt actaaacaat caacgtcagg
   138541 caaccaccac accgccaaca ttgacactga ttactgatga cagtcactgg acactgcgtt
   138601 tcccgcatga ctggtttagt cagaatgcgc tggtactgct tgatctggaa aaggagcaag
   138661 aatactggga aggcgtggct ggctggcggt tgaaaattga agaagaaagt accccagaaa
   138721 tcgccgctta atgctttgcg ggccgacgag aaatgtcggc ccgcattatt caggcacttt
   138781 cgcgaatggg ttcgatttca ttcagcgtat caattaacgg ttgcggctta ccaataagat
   138841 aaccctgcat ataatcgatc cccaaagaga gcaccgcctc gcggatctct tcgttttcaa
   138901 cgtactctgc cactacccgc attttcttca ttcgcgccag atggcaaatc gacgccacaa
   138961 tctgataatc cagactattt gacacaatat tgcggataaa gctgccgtca attttaagca
   139021 gatcggcatt cacattttta agccgtgcat agctggcgta gccggtgccg aaatcatcaa
   139081 tcgcaatctg gcagcctaat tcctgaagat gctgcaaggt gatttgcgcc tgcttaacat
   139141 tggtcagagc attactttcg gtgacttcaa aaataagttg ccacgcttca atctgatatt
   139201 tagccagcaa ctgactgact tcaacaggaa aacgagcctg acataccgag gttggagaca
   139261 gattaatagc aaaacggtga gcgggcatct tcgctctgtt ttcagccata aattgcagcg
   139321 tatgctcaat gacccacatg tcgatactcg acgataaacc aaattcgtgc gcgaccggca
   139381 agaagctatc ggggccgatc agttcatcat tctcaccttt catgcgcaga agaatttcat
   139441 ggtaaacatc accacgcata ccggtaatcg gctgggccat caggaaaaaa tggttgtgct
   139501 ccagcgcctg ctgtagtcga ttcatcatcg cgactttatc tttcaattcg cgttgcaaat
   139561 acattgcccc gcaacgctgc atattttccg gggcgttggt cacgatggaa agttcggcga
   139621 ccgtatttag ctctcccagc agcaggtaga tatgattcac tggcgagcgc acatagcagt
   139681 aactgacgcc aatctgcggt tgcatcggca tcccatccca aaagaaacga aattgcttga
   139741 gatgactatc cagtgc
//
LOCUS       DAJTYG010000002        85586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTYG010000002 DAJTYG010000000
VERSION     DAJTYG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 85586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.002"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..1582)
                     /locus_tag="OO568_000133"
     CDS             complement(<1..1582)
                     /locus_tag="OO568_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6639639.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYMFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGT"
     gene            complement(1566..1922)
                     /locus_tag="OO568_000134"
                     /pseudo
     CDS             complement(1566..1922)
                     /locus_tag="OO568_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(2045..2227)
                     /locus_tag="OO568_000135"
     CDS             complement(2045..2227)
                     /locus_tag="OO568_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639640.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(2211..2651)
                     /locus_tag="OO568_000136"
     CDS             complement(2211..2651)
                     /locus_tag="OO568_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6639641.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(2651..2947)
                     /locus_tag="OO568_000137"
     CDS             complement(2651..2947)
                     /locus_tag="OO568_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR6639642.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(2944..3282)
                     /locus_tag="OO568_000138"
     CDS             complement(2944..3282)
                     /locus_tag="OO568_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6639643.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(3279..4490)
                     /locus_tag="OO568_000139"
     CDS             complement(3279..4490)
                     /locus_tag="OO568_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6639644.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLSAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(4492..5064)
                     /locus_tag="OO568_000140"
     CDS             complement(4492..5064)
                     /locus_tag="OO568_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6639645.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFSFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPREDNRRWWADLAGV"
     gene            complement(5104..6261)
                     /locus_tag="OO568_000141"
     CDS             complement(5104..6261)
                     /locus_tag="OO568_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR6639646.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGNTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            complement(6564..6725)
                     /locus_tag="OO568_000142"
     CDS             complement(6564..6725)
                     /locus_tag="OO568_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6639647.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(6735..7040)
                     /locus_tag="OO568_000143"
     CDS             complement(6735..7040)
                     /locus_tag="OO568_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639648.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(7037..7315)
                     /locus_tag="OO568_000144"
     CDS             complement(7037..7315)
                     /locus_tag="OO568_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639649.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(7507..9255)
                     /locus_tag="OO568_000145"
     CDS             complement(7507..9255)
                     /locus_tag="OO568_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6639650.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRPQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(9252..9551)
                     /locus_tag="OO568_000146"
     CDS             complement(9252..9551)
                     /locus_tag="OO568_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6639651.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(9557..9790)
                     /locus_tag="OO568_000147"
     CDS             complement(9557..9790)
                     /locus_tag="OO568_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639652.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(9792..10012)
                     /locus_tag="OO568_000148"
                     /pseudo
     CDS             complement(9792..10012)
                     /locus_tag="OO568_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10005..10226)
                     /locus_tag="OO568_000149"
     CDS             complement(10005..10226)
                     /locus_tag="OO568_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639653.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(10216..10416)
                     /locus_tag="OO568_000150"
     CDS             complement(10216..10416)
                     /locus_tag="OO568_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6639654.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSTGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            complement(10425..11171)
                     /locus_tag="OO568_000151"
     CDS             complement(10425..11171)
                     /locus_tag="OO568_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639655.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWNGGLPRLSFLCVDTLITPHVMYL
                     CVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            complement(11229..11417)
                     /locus_tag="OO568_000152"
     CDS             complement(11229..11417)
                     /locus_tag="OO568_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6639656.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(11792..13021)
                     /locus_tag="OO568_000153"
     CDS             complement(11792..13021)
                     /locus_tag="OO568_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6639657.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            13270..14391
                     /gene="roxA"
                     /locus_tag="OO568_000154"
                     /gene_synonym="ycfD"
     CDS             13270..14391
                     /gene="roxA"
                     /locus_tag="OO568_000154"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6639658.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(14440..15666)
                     /gene="pepT"
                     /locus_tag="OO568_000155"
     CDS             complement(14440..15666)
                     /gene="pepT"
                     /locus_tag="OO568_000155"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6639659.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVAEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTNGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            15916..17052
                     /gene="potA"
                     /locus_tag="OO568_000156"
     CDS             15916..17052
                     /gene="potA"
                     /locus_tag="OO568_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6639660.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17036..17893
                     /gene="potB"
                     /locus_tag="OO568_000157"
     CDS             17036..17893
                     /gene="potB"
                     /locus_tag="OO568_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6639661.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            17890..18684
                     /gene="potC"
                     /locus_tag="OO568_000158"
     CDS             17890..18684
                     /gene="potC"
                     /locus_tag="OO568_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6639662.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            18681..19727
                     /gene="potD"
                     /locus_tag="OO568_000159"
     CDS             18681..19727
                     /gene="potD"
                     /locus_tag="OO568_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6639663.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSQLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(19883..20704)
                     /gene="cobB"
                     /locus_tag="OO568_000160"
     CDS             complement(19883..20704)
                     /gene="cobB"
                     /locus_tag="OO568_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6639664.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(20720..21631)
                     /gene="nagK"
                     /locus_tag="OO568_000161"
     CDS             complement(20720..21631)
                     /gene="nagK"
                     /locus_tag="OO568_000161"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6639665.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWEDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(21660..22904)
                     /gene="lolE"
                     /locus_tag="OO568_000162"
     CDS             complement(21660..22904)
                     /gene="lolE"
                     /locus_tag="OO568_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6639666.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(22904..23605)
                     /gene="lolD"
                     /locus_tag="OO568_000163"
     CDS             complement(22904..23605)
                     /gene="lolD"
                     /locus_tag="OO568_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6639667.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(23598..24797)
                     /gene="lolC"
                     /locus_tag="OO568_000164"
     CDS             complement(23598..24797)
                     /gene="lolC"
                     /locus_tag="OO568_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6639668.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            25059..26105
                     /locus_tag="OO568_000165"
     CDS             25059..26105
                     /locus_tag="OO568_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6639669.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIFSRLALPLFA
                     LFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLRRHLLMASLL
                     TVLAVGAWLFTISLLLSLVSTVVIMKLFYQYEQRFGMRSTSLLNVIGSNTIAIYTTHR
                     ILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLL
                     FSPPSLPAAVSYSR"
     gene            26233..29679
                     /gene="mfd"
                     /locus_tag="OO568_000166"
     CDS             26233..29679
                     /gene="mfd"
                     /locus_tag="OO568_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6639670.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRYVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     SERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLNDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGMIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            29822..30784
                     /gene="ldtC"
                     /locus_tag="OO568_000167"
                     /pseudo
     CDS             29822..30784
                     /gene="ldtC"
                     /locus_tag="OO568_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(30867..31124)
                     /gene="bhsA"
                     /locus_tag="OO568_000168"
     CDS             complement(30867..31124)
                     /gene="bhsA"
                     /locus_tag="OO568_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR6639671.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            31365..31997
                     /gene="comR"
                     /locus_tag="OO568_000169"
     CDS             31365..31997
                     /gene="comR"
                     /locus_tag="OO568_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6639672.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(32059..32598)
                     /locus_tag="OO568_000170"
     CDS             complement(32059..32598)
                     /locus_tag="OO568_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6639673.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(32825..34129)
                     /gene="ndh"
                     /locus_tag="OO568_000171"
     CDS             complement(32825..34129)
                     /gene="ndh"
                     /locus_tag="OO568_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6639674.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(34530..35072)
                     /locus_tag="OO568_000172"
     CDS             complement(34530..35072)
                     /locus_tag="OO568_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6639675.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(35095..36120)
                     /gene="nagZ"
                     /locus_tag="OO568_000173"
     CDS             complement(35095..36120)
                     /gene="nagZ"
                     /locus_tag="OO568_000173"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6639676.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(36131..36955)
                     /gene="thiK"
                     /locus_tag="OO568_000174"
     CDS             complement(36131..36955)
                     /gene="thiK"
                     /locus_tag="OO568_000174"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6639677.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(36936..37577)
                     /gene="lpoB"
                     /locus_tag="OO568_000175"
     CDS             complement(36936..37577)
                     /gene="lpoB"
                     /locus_tag="OO568_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6639678.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(37592..37969)
                     /locus_tag="OO568_000176"
     CDS             complement(37592..37969)
                     /locus_tag="OO568_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6639679.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(37972..38331)
                     /gene="hinT"
                     /locus_tag="OO568_000177"
     CDS             complement(37972..38331)
                     /gene="hinT"
                     /locus_tag="OO568_000177"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6639680.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            38665..39489
                     /locus_tag="OO568_000178"
     CDS             38665..39489
                     /locus_tag="OO568_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6639681.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPT"
     gene            40018..40854
                     /locus_tag="OO568_000179"
                     /pseudo
     CDS             40018..40854
                     /locus_tag="OO568_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563795.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            complement(40914..42347)
                     /gene="ptsG"
                     /locus_tag="OO568_000180"
     CDS             complement(40914..42347)
                     /gene="ptsG"
                     /locus_tag="OO568_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6639682.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(42642..43439)
                     /locus_tag="OO568_000181"
     CDS             complement(42642..43439)
                     /locus_tag="OO568_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6639683.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLCDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(43450..44454)
                     /gene="holB"
                     /locus_tag="OO568_000182"
     CDS             complement(43450..44454)
                     /gene="holB"
                     /locus_tag="OO568_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6639684.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWVATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(44451..45092)
                     /locus_tag="OO568_000183"
     CDS             complement(44451..45092)
                     /locus_tag="OO568_000183"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6639685.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARTRGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(45082..46104)
                     /gene="yceG"
                     /locus_tag="OO568_000184"
     CDS             complement(45082..46104)
                     /gene="yceG"
                     /locus_tag="OO568_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6639686.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(46107..46916)
                     /gene="pabC"
                     /locus_tag="OO568_000185"
     CDS             complement(46107..46916)
                     /gene="pabC"
                     /locus_tag="OO568_000185"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6639687.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(47036..48277)
                     /gene="fabF"
                     /locus_tag="OO568_000186"
     CDS             complement(47036..48277)
                     /gene="fabF"
                     /locus_tag="OO568_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6639688.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(48364..48600)
                     /gene="acpP"
                     /locus_tag="OO568_000187"
     CDS             complement(48364..48600)
                     /gene="acpP"
                     /locus_tag="OO568_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6639689.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(48811..49545)
                     /gene="fabG"
                     /locus_tag="OO568_000188"
     CDS             complement(48811..49545)
                     /gene="fabG"
                     /locus_tag="OO568_000188"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6639690.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(49558..50487)
                     /gene="fabD"
                     /locus_tag="OO568_000189"
     CDS             complement(49558..50487)
                     /gene="fabD"
                     /locus_tag="OO568_000189"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6639691.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(50503..51456)
                     /gene="fabH"
                     /locus_tag="OO568_000190"
     CDS             complement(50503..51456)
                     /gene="fabH"
                     /locus_tag="OO568_000190"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6639692.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(51524..52594)
                     /gene="plsX"
                     /locus_tag="OO568_000191"
     CDS             complement(51524..52594)
                     /gene="plsX"
                     /locus_tag="OO568_000191"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6639693.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(52675..52848)
                     /gene="rpmF"
                     /locus_tag="OO568_000192"
     CDS             complement(52675..52848)
                     /gene="rpmF"
                     /locus_tag="OO568_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6639694.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(52900..53421)
                     /gene="yceD"
                     /locus_tag="OO568_000193"
     CDS             complement(52900..53421)
                     /gene="yceD"
                     /locus_tag="OO568_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6639695.1"
                     /translation="MQKVKFPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            53620..54204
                     /gene="yceF"
                     /locus_tag="OO568_000194"
     CDS             53620..54204
                     /gene="yceF"
                     /locus_tag="OO568_000194"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6639696.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(54316..55275)
                     /gene="rluC"
                     /locus_tag="OO568_000195"
     CDS             complement(54316..55275)
                     /gene="rluC"
                     /locus_tag="OO568_000195"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6639697.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            55848..59033
                     /gene="rne"
                     /locus_tag="OO568_000196"
     CDS             55848..59033
                     /gene="rne"
                     /locus_tag="OO568_000196"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6639698.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGNDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(59230..60183)
                     /gene="flgL"
                     /locus_tag="OO568_000197"
     CDS             complement(59230..60183)
                     /gene="flgL"
                     /locus_tag="OO568_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR6639699.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMLTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRTELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(60195..61837)
                     /gene="flgK"
                     /locus_tag="OO568_000198"
                     /pseudo
     CDS             complement(60195..61837)
                     /gene="flgK"
                     /locus_tag="OO568_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(61903..62844)
                     /gene="flgJ"
                     /locus_tag="OO568_000199"
     CDS             complement(61903..62844)
                     /gene="flgJ"
                     /locus_tag="OO568_000199"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6639700.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDQVSKTYSMNIDNLF"
     gene            complement(62844..63941)
                     /gene="flgI"
                     /locus_tag="OO568_000200"
     CDS             complement(62844..63941)
                     /gene="flgI"
                     /locus_tag="OO568_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6639701.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAVVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(63953..64651)
                     /gene="flgH"
                     /locus_tag="OO568_000201"
     CDS             complement(63953..64651)
                     /gene="flgH"
                     /locus_tag="OO568_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6639702.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(64704..65486)
                     /gene="flgG"
                     /locus_tag="OO568_000202"
     CDS             complement(64704..65486)
                     /gene="flgG"
                     /locus_tag="OO568_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR6639703.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(65624..66375)
                     /gene="flgF"
                     /locus_tag="OO568_000203"
                     /pseudo
     CDS             complement(65624..66375)
                     /gene="flgF"
                     /locus_tag="OO568_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(66395..67603)
                     /gene="flgE"
                     /locus_tag="OO568_000204"
     CDS             complement(66395..67603)
                     /gene="flgE"
                     /locus_tag="OO568_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6639704.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNVDSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(67628..68323)
                     /gene="flgD"
                     /locus_tag="OO568_000205"
     CDS             complement(67628..68323)
                     /gene="flgD"
                     /locus_tag="OO568_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6639705.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(68335..68738)
                     /gene="flgC"
                     /locus_tag="OO568_000206"
                     /pseudo
     CDS             complement(68335..68738)
                     /gene="flgC"
                     /locus_tag="OO568_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(68742..68915)
                     /locus_tag="OO568_000207"
     CDS             complement(68742..68915)
                     /locus_tag="OO568_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HCR6639706.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVTQFADNSLQYQMSLSALSG
                     QIKGMMNVLQSGN"
     gene            69070..69729
                     /gene="flgA"
                     /locus_tag="OO568_000208"
     CDS             69070..69729
                     /gene="flgA"
                     /locus_tag="OO568_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6639707.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            69805..70098
                     /gene="flgM"
                     /locus_tag="OO568_000209"
     CDS             69805..70098
                     /gene="flgM"
                     /locus_tag="OO568_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6639708.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            70103..70519
                     /gene="flgN"
                     /locus_tag="OO568_000210"
     CDS             70103..70519
                     /gene="flgN"
                     /locus_tag="OO568_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6639709.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDHEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(70559..72094)
                     /gene="murJ"
                     /locus_tag="OO568_000211"
     CDS             complement(70559..72094)
                     /gene="murJ"
                     /locus_tag="OO568_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6639710.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(72261..73127)
                     /locus_tag="OO568_000212"
     CDS             complement(72261..73127)
                     /locus_tag="OO568_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6639711.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(73129..73776)
                     /locus_tag="OO568_000213"
     CDS             complement(73129..73776)
                     /locus_tag="OO568_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6639712.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTLGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(73787..74371)
                     /gene="rimJ"
                     /locus_tag="OO568_000214"
     CDS             complement(73787..74371)
                     /gene="rimJ"
                     /locus_tag="OO568_000214"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6639713.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HTCYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            74607..75815
                     /gene="mdtH"
                     /locus_tag="OO568_000215"
     CDS             74607..75815
                     /gene="mdtH"
                     /locus_tag="OO568_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6639714.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSTHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            75879..76526
                     /gene="grxB"
                     /locus_tag="OO568_000216"
     CDS             75879..76526
                     /gene="grxB"
                     /locus_tag="OO568_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6639715.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            76660..77220
                     /locus_tag="OO568_000217"
     CDS             76660..77220
                     /locus_tag="OO568_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6639716.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            77326..78372
                     /gene="pyrC"
                     /locus_tag="OO568_000218"
     CDS             77326..78372
                     /gene="pyrC"
                     /locus_tag="OO568_000218"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6639717.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLQTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHTRHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            78446..78691
                     /gene="dinI"
                     /locus_tag="OO568_000219"
     CDS             78446..78691
                     /gene="dinI"
                     /locus_tag="OO568_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6639718.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            78981..79235
                     /gene="bssS"
                     /locus_tag="OO568_000220"
     CDS             78981..79235
                     /gene="bssS"
                     /locus_tag="OO568_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6639719.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            79350..80468
                     /gene="solA"
                     /locus_tag="OO568_000221"
     CDS             79350..80468
                     /gene="solA"
                     /locus_tag="OO568_000221"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6639720.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            80516..80629
                     /locus_tag="OO568_000222"
     CDS             80516..80629
                     /locus_tag="OO568_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR6639721.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            80890..81456
                     /locus_tag="OO568_000223"
     CDS             80890..81456
                     /locus_tag="OO568_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6639722.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            81460..82035
                     /locus_tag="OO568_000224"
     CDS             81460..82035
                     /locus_tag="OO568_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6639723.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(82077..83129)
                     /locus_tag="OO568_000225"
     CDS             complement(82077..83129)
                     /locus_tag="OO568_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6639724.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            83354..84274
                     /gene="lpxL"
                     /locus_tag="OO568_000226"
     CDS             83354..84274
                     /gene="lpxL"
                     /locus_tag="OO568_000226"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6639725.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGSWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            84446..84643
                     /locus_tag="OO568_000227"
     CDS             84446..84643
                     /locus_tag="OO568_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6639726.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            84667..>85586
                     /locus_tag="OO568_000228"
     CDS             84667..>85586
                     /locus_tag="OO568_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6639727.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYWDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEEILRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIKCS"
BASE COUNT        20528 a        22801 c        21085 g        21172 t
ORIGIN      
        1 atgtaccaaa cgccatcagc gccgccacgc tcgggtctat cttgttcggt gattttttct
       61 tgttcggctt gatattggcg ttcgcgtcac tctgcatcac gacgttactc atcgaccagg
      121 acaacaccgg atcgccacga tgcacaatca ccctgcggtt aacaaaaact tcgaacgatt
      181 tcgccgccgg actgaatctg aggtaggttt gcgggaacgg ctccacctca aaaccagccc
      241 cctgtaattg cgtcctgaga tgcgtggcgt tccacgtatc aaagcccacc agcctgatat
      301 taaatttctc cgcatcctgc atgatgtcat ctctgatccg gtcgtaatca atgcagtcac
      361 ctggcgttgt gcgtatccag cccgctttag cccactggcg atagacagcg cggtttttat
      421 tggcggggtt ctgtagctgg aactccggca gataatgacg ggaaatcagc ataatggttt
      481 taccgaccgg aaaggcatag catacgctgg aaatatcgct ggttgatgat aagtccagcc
      541 ccgcgtaaca ctcctgaccg tgtaaatctt cctccgtgaa cgttccggca cactcagccc
      601 atgcaccatt acccatccac ggggtagtcc cctgacacca gatattaaat cgctttgtca
      661 tcatctccac ccactgcgac ggaatacccc gcgctttctg gatggttgag gccagttttt
      721 cacgatccac ggaaacatca atattagggt tcgcctttat ccacatcgcc ggatcatcaa
      781 cctcgttttc gtcgtccagt tcgtaaatca gtacaaaaat tgaatcgttg acctcttcgc
      841 cgtccaggat ctggcagcaa taatcatagt gctgtttaca ggctgaaacg acgttactgc
      901 ccgatgtggt aatggcaaat aacagccctt cgggacgtgc gcccatcccc agctcaagcg
      961 cggaataaac gccgttatcg gggtgtaggt ggtattcgtc cacgatggaa agactggggt
     1021 ttgtcccctc gatggttgcc gcttttgctg ccagcggctt taacaggctg ttgcttttcg
     1081 ggtgtatcac cttatgcgcc tgaatattca cccgcctgcg taacggtcgg gataaaaggc
     1141 acatctgacg cgcatcatca aacacgatcc gcgcctggtc acggctcacc gctgcggtgt
     1201 agatatcctg ctgaccgttc tccataatca gaaaccagtt agccagaatc gcggcggtcg
     1261 tggatttcgc atttttgcgc ggcacttcga taaaggcgct cgtgtatttg cgccgtccgg
     1321 tggccttaac cttaaagccg aggatgcacg caaaggcgaa ctgctgccac ggctccagct
     1381 caatgggtct gccacgcatc ggtcctttta cgtgcgggca caccctggaa aaggcaataa
     1441 accgctccac aacctcacga tcgaacatgt aaagggggct tttaaggtcc gaaaagtacc
     1501 gtttaacagc ctgttttaac cgtttacagg ccggaatttt gcccgttttt acgtcttctg
     1561 cgtacttatt ccaggcggtc aagctcgtcc tcttcttctg tttccggtgg atttttacgg
     1621 cggcttatcg ggtcaaaacc gagcaaggag gccattttta tcatcactct ttcagcgtcg
     1681 gattttgcgc ttaatgcggg gtttctgctc tcgccgccct gactgttaac aatgctgaac
     1741 ccgcgcgccg caaggtctgc aacggctttc cggtaaatgg attagttgac gcaatacagt
     1801 tccagattgc tccagtcggc gggggtcagg tctccccgtt ccgcaagctg ccgcgatttt
     1861 tcccgccact gcttcacggc gatatcatcc aggtaggcgg gggctttcgg tggtcttgcc
     1921 atgcttattt ttcgtcagat tatttttcaa aaaattcccg tgcataaaaa tttggggagg
     1981 cgttcggtgc ccggcggggt cgggtttgtc ctgaaaacgc cccccacccc gtcatacagc
     2041 ctcatcagcg attgcggaaa cattccataa cctcgcggtc acggtcggtt aatcgcttcg
     2101 ctgtgatgcg ttctgcgcgt cctgacgctt tatctttatg ccctgtttcc tgtgtcttcc
     2161 atgcgtcacg ctgccttata agtccacgga taaggcggtt ttgttcccgc tcattcatca
     2221 gcgtcataca tccagttatt gcggttagca gcccgttctt cctcttcacg aaatccacct
     2281 gcggcacgct tgctttttgt tgccggatca agccatttcg ttttctggtt atgacacgcc
     2341 tggcacaatg gttgatggtt ccattcgggc cagaagagaa catcatcacc gccattaatc
     2401 gggataatgt gatccaccac cacggcgggc gtatatatcc ccttctcaag gcatcgcacg
     2461 cataacgggt ttttactcag atacatggcg cggtatttgt cccactgtct ggaatatcca
     2521 cgcgcgcggc ggtgtcctcg tctggcatcc tctgcacgcc atgccgcccg cctgtgctcc
     2581 tcacacttgc cggatttaac gcgcctgtta cagcctggtt ctgtgcatcg tcttaatggt
     2641 tgccacggca tcagtacacc cccacatcac ggtaagccgt ccagagtgcg cctatcgtca
     2701 tgggtacgcg tgtttttgcg ttatccgcga caatctggcg attttcatac aggtgagcga
     2761 taaacatcat gcagccaatc tttatggctg gcgtgaacgc cagcccgtca tcaaagcgcc
     2821 tgcctatgtg tatctggcac gcctcaagcg atgcggcaat gtatccgctg attaactggt
     2881 cttcatcgtc gccatcgatg cggcaatgga gtttcacttc ttccagggtg ataagttctt
     2941 ctgtcatttt tccgcgccct cacgacaaag aatttcaagg cgtgtcctgg cggcatccgg
     3001 cagcggctgc ccgatgatat tcagtacacg ccccgccagc ggccccgtac tgacctttat
     3061 ccggctggtg gcgttgatgt ccttccggta ccgtatccag atccttacgg taccaatcgc
     3121 cagctctgcg cccgatgaaa tggcctcctt gctgctgacc atgttcacgc ttgcccagag
     3181 tgtgtgaccg tcctcccacg tttcgagtat ttcgccggtt atatccctgg tctgtttcag
     3241 ggtctgaatc gttatcctgt cacgcagtcg ccctatgttc attccggttt ttctccctcg
     3301 ctgattttta cttcctgttt ccatgcctgg ctgaactcat caccaccatc acgcggtgac
     3361 agcccttcac gttcgcgggc ttcgttcgga cacataacgc cggattttat gccgcgctca
     3421 taggtggcaa agcgttcgcc aggtgtggcc cgtaacaggt ccgcgctgtc aaactccacc
     3481 tgataccgga taccaggtac aggcgaggcc accagcaagg cattttttat ctgctgctca
     3541 aagttcgcca gccacgggcg catcgtcatg gtgagaaatg cgcggcttgc ctcgctgaag
     3601 ttgctgtagg tgctgttgct gtattcctgc aaaaaaatcg gcgagacgtt gaacattcgg
     3661 gcgatgtctt caatggtgaa gcgacgggag gccagccatt cggcatcctg attactcatg
     3721 cccagctgct gatagctcat ccccccttca aggatgggcg tttttccggc gtttctggcc
     3781 cctttgtagc gttccagtgc ggctaatgcc tgtttgccct tcacgccgtc cagccattcg
     3841 cctgacgtga taacccctgc cgccatcatg ccatcacgca tcacgctcgc gccgtggcgt
     3901 tgttgggcca gcccaagccc cagcgattcg cggcagatgg ttacaggtga gcgccccata
     3961 aaaccgtcat ccgtggagta acgaaggtgg agaacctccc acggtaaata gttgcgggtg
     4021 tttccggtgt aggcgtcagt gatgcagtag cgccagttat gatcacctgt ctgctccacg
     4081 ttcaccgact gcggcgggta aggatgtaaa gccgccggaa aaccatcacg cccccactga
     4141 ataaccgcat aagcattacc acttaacagg cagtggcgga tcatcattcg cttaaactgg
     4201 tagggtgtct gccacgcgtt cgggcgctcg ttgaggatgt gatcgaccgg atgagaatca
     4261 agccattcgc gggcctcctt ccccttctca ttgcgtacca ggtacaggta acacggcatg
     4321 gtagccaccg cctcagagat gaccgtgacg gcgttcatga cggcggagag tgattccgct
     4381 gtcccagatg atacgtactc acccgccccc gtgttcgatg tgccggccag cgccataaat
     4441 tcatcaagcg tcatgctgcg ctgctctttt tttcgtctga aaggccacat atcacacccc
     4501 cgctaaatcc gcccaccagc ggcgattatc ctcacgcggc attttttcgg ggtgctgctc
     4561 atacagggaa cggcgggcca gctccacgcc ggaatcgggg taagccggta ccgatgtaac
     4621 ggtaatttcg tacagttccg ccaccagcac ggtgcgcacg catggatctg ttgtggtatc
     4681 ccatacatcc ttacgggagc gaaagctaaa gctcatcccc gagatgtccc cacgcttaac
     4741 cagttcgata acgtcgcgcc ctgtactggt atccggtggg gtcagttcaa agcgtaaccc
     4801 tgtttcgtcc tcttccagtt tcagcgtgcc ggaacgggtg cgccccagta acatgctgtg
     4861 gtcatgctca tacaggccgc gaacgtcatt acccgccgca agccattcag taaacgcccc
     4921 ccgctggaat ttttcgtaaa actcacccca taacagttct gaaagcttat cccagcgaac
     4981 aacgtagccc gtcagcgtac cggctccgct ggtggtgatt tccgatgacc ggatttccat
     5041 actcttcata attttttcac ccataaaaca ctaaaggggc tttttagccc cttctgtatg
     5101 ctgttaatcg tcgtcttccg gcagttccag aatcttgatc gcgttcgaat ccaccacgcc
     5161 accgcctaaa tatttttgcg tgaaaatttt gatgaatcct ggttctgtca ggttgtccgg
     5221 tctggtgcga acacctgttt cgtgatcaac aatgtagtaa ccgcgtttga agtcacccag
     5281 ggcaataacg ttatcaggca taaactccag atattcgacc ggaaggccca gcaacgtatc
     5341 aggatcaccc gcctgtaaac ggtcgcgcca gatataatca ccgttcgcgt tcttcacctt
     5401 ctgaagtttt gccgccgtcg tggagttaac cacccagacc gcgtttttgc ggtatttttt
     5461 acgtaatgca aatttcaggt cgatcagcgg gtccgcagat gtccacgcca gagattcgga
     5521 aggtttaatt acctgcaacg taccaaaatc acgctctttg tcgttcttct ctgcacgggg
     5581 tacggataaa aaaccttttg cttttttgtc accgtcgccc acaaccagat cgctttcttc
     5641 ggtttccgtg aaggtgttgc caatctcacc cgtcagccat gaaaggatgt ccacatcgga
     5701 aaaatccacg atttcctgtg tggtgcgcgg gtacgcatag accggataca gcttaatgct
     5761 cacctcgtta atctgcgggg tgctggtctg ttcgcgtgcc ttaccctctt caccgtggtt
     5821 aacggtcgca ccgccagcgg aaacaagctg cttaaactcg ttgctgctga ttttcttcac
     5881 ggtacagatg cggcgcatgg tggattcatc cgccagcatt cgcatgattt cggtgttcag
     5941 ttcagggata acggtataac caccatcagc gggaacgcct gtacttaatg cgcgggtttc
     6001 accggtcaga atgtagttgc gtagttctgc ggggtcagtg gtctggctgc ttttacctgg
     6061 cttgctgcgc tcttcgtctg caatggcttc aaggcgggaa atgtctttat cgagggattc
     6121 agctttagcg cgtaattcgt caaattttgc gccctcagca tcgttaagac tgcggttttc
     6181 tttttcagcg ttctccagca tgtcgcgcat ctgattttta atggcggttt tctgctggcg
     6241 taattcgatt attctctgca taaaaaagtc ctggggttaa gtaaggaact ccaggacgcg
     6301 gcaaacactc aaccgttttt cataaggaaa tcagtaatcg caccgatcgt tttcccgcct
     6361 ggtaatgaat attggcgagc acattaacag gcgggaaagt ggccccagcg tcctggcacc
     6421 acgggcgaga ataatcatga ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa
     6481 cgtggaacaa ccacgaacaa ataatttaca aaaaataaca aaaagccgga ttgctccggc
     6541 tgcgtggtgt ggttctgata ttcctactct gctaatttgc tcagccccat gtctgcgtaa
     6601 gtgcgattta ctgcatttct caggtctgcg tagttctccg ttggcggctc cggtgtgccc
     6661 ttgctgctct gttttaccag cgggtcgccc accagccgcc cgtaagctgc aatttgtgct
     6721 gtcatattgc cacctcagtg aagcgatccg gcattgttct ttttcatctc ttccatcatt
     6781 ttttcagtga gcattacgca ctgaaacccc atgtttttga tgtctacggg tttcatgcct
     6841 ttgtcagtgg atatcacagg cagaatatcc tgatacacat cgcggtcagg gtaactaagc
     6901 acacaaagcg cccatgatgc cacgggtgag gatccttcgt ctgtttcaac ccaccagcct
     6961 ggtgtagctg cgcttatttg cagaatcgtg atccgtccgt tgtgcttgta aaatttttca
     7021 ccaaattttg gttcgctcat gattccacct ctccggtttt aacgttgatt gttgttacct
     7081 gttccgcttc ggcaatctcc cgctctgtca gcgtggcaaa atttgccgcc gtcgtggtca
     7141 tgaatgcgct tatcagttcg ggatgtgctt tcgcgtatcc ttcccccgcg tggcggtcta
     7201 tcgttctgat tgccaccttt aaagcgtgct ctgtcatgtc taacgcgcga tatttcggtt
     7261 ctgttctgtc tctgcgtttt ttgagtgatt tattaaattt ccctgaagtg tgcatattta
     7321 tttttacccc ctcgtttaaa aagttttgag ttgtgcctcc ccttgtctac cttagtggcc
     7381 ctcatgccag taatggcgct gctttcagcg gggtagagtg cttttatcca ctatctaccc
     7441 cgtgtctacc tccctgtctg attcaggtaa aatcaggtag acagggtaga cagcaattac
     7501 aaaaaattat aaaaacgcgt cgcactcgtc tgttgttatt gcgttagtct gcgttactcc
     7561 cttaactttc cgcgtaatat attcatgtcc gtaaactttc gcggctggct tcatagcctt
     7621 gccaaagtca tttacgttta gcggtttgct cctgcctgcg tacgccataa acgccagata
     7681 gacgcggtaa aggctgtttc tggtcgtgta cttcactgaa tcgccaccgc cgcccatcat
     7741 caggccgcgt gcttcctcca gaaaattcag gaactggcaa aactcaataa ccggatccgt
     7801 ctgttgcttt attgccagag cttcatcacc gtcacgctgt tccagtagta aagcccgtgc
     7861 tttctcagag tcggtaaagt tcgccagcaa tcggcggata atgacaggga tttcagccgc
     7921 aatcttttcc ggtagctccc tgtctttttc ggcctcacta acgatattgt cgaaacggaa
     7981 aatcacgcga cgacgtgcca cacctccggc ccgttcggtg aatatcatcg ggttattgtt
     8041 ggtcgccagc accaccgccc tgattacagc cgtgaaacgc ttttcgtatt tcgggttaat
     8101 ttccacgggg tcaccgcccg tgattttctt gatgcccgtt ccttcgcctg tatatttcgg
     8161 ctggtcagcc aggacgataa gacgactccc gacaacctgc ggacgtccac cagcatcatc
     8221 aagcgatgtc atttcagcgc ttaccgtgtt ctgtttccct gccagaaggc tggctatgtg
     8281 tgtgaatgta cttttaccgc tcccgccgtc tccggtggcc tcaataaaca tctgccagtc
     8341 gtaccggttc gccataatca tgtacagcgc ggcacatata cgcatcatct tgcgctggtc
     8401 ttttccggct gcgtgctcaa gccatttatg aaagtttagc gcgttatcgc ggatgttctc
     8461 ccctggtgct ggtggcgtgt actcaatgcc gttgtgcgtg gtgatccagt tctccggcgt
     8521 gtgcggggaa aattcccccg ttttcaggtc aagcgcacca ttggcgaacg gcagcaaatc
     8581 gccggacggc tcgcccattg gttcggcaat aacttttaac gcttccacgg cgttattgat
     8641 tacgcgcttg ctgaaagtgg ccctgtgctc tgaatagatc gccaccattt cgcggctaag
     8701 ttccattgtg ctgaccggac accatacccc gccgcgccat acgtgaacga tttcactttc
     8761 ctgatgcaca caaacgccat caaagcgctc ggcaagcagc tgcgcgcgct cactgtctgc
     8821 catttgtgcc agttgcgtat ttttctgctc cggcatggta agccctgcgg caatattttc
     8881 acgctcagca ttcagatagc gccgccagtt ttcacacttc tggacgtgca ttccttcggg
     8941 gtaaaaattt gcgtcctgaa tatctgccgc cgccagcttc tgaccaatct ttttggtctc
     9001 caccaaatcc agttctccgg cctggtacag ccttacgcgt ttttttccat ccggaacaat
     9061 tttcagtgca tcaagttcgg caagctgatt tggcccaagc cacacaggcg gcacattatc
     9121 gccggatgcg gggccatcct gctcctgcca ttgtttcgcg tgcgaccagg catcactacc
     9181 cgcaaagata attacctctg tgtctttgtg ttttattccg cgtggctgtt tttttacgtt
     9241 cggtgccagt ttcatttttt acccctgaat acgttaagca tcttttttat ttcctgaata
     9301 ttggcgcgtg ctttctccct gctggttggt ttactgcggg gcgctgcctg taccagcgtg
     9361 aaatcacgcc ggaattgata aacaggcatc acgcaatcat attcgtaacc ttcacggcgg
     9421 taggtgatgc gccgttctgc cacgccttta atcgttacca tgccgccgta ctggtcgcgg
     9481 taaatatcac cgcgcgtaaa tttagggtga gtgttgccac tggcagttaa gccagaatat
     9541 ttaagtttca ttatttttat tctccggtgt gctgttcttt atatctgtcg tgcaatagat
     9601 ctatttcttg cagttccatt attacaggct caagaagcgt tattaatgcc gtggcaattc
     9661 ttgatttttg tttgtcgcgt tcattgtcgc caagtgtttc aagccatatg cgcaatattt
     9721 ccagcatgtt ttcactgtga gaaagtgcaa gaaatgcgcg gtctattgtt tcgtggtaaa
     9781 tatcacgcat attaatcccc gtccgtcgtt tttcttaaaa taacctctgt cacgaaatca
     9841 gcataatcgg cggcgatatt cagtacatca agcccgttga tttatattct ctcgtggaaa
     9901 gtaagaaaaa agccgctcta ataagttctg gcattgacga aagcgcatca gccgcatcat
     9961 caggaacgcc ggaaaattcc tgtttcaggg aattaaaacg atcatcacgc ataacccccc
    10021 ccattttcac aatcggcaac aagaatattt ttagcgtcat tcagtgaccg cgttgcgatg
    10081 tatcggatac attccagggc gaactgtgtc cggtctttct gttcttctcc ctgcgcaagc
    10141 tctgccgtgc attcaatatc aataagcgcg tgcatcagcg tagtgagtgc ggcggctgct
    10201 gcgttcggtg tggttttatt gtgcataggt ttccccctga cgaatacggg cggcgaatac
    10261 agcaacacaa ccggacgggc aacggctacg cgcttcgcgt tccgtccaag cggttacgtg
    10321 gatgatttgg gattctccgg tactcagtgc cagaaaacgc cacacaaagg ccgtttgtgt
    10381 gtgtacaagg tgtggtatat gatttacggc aaccataacg gctcctagtt tacgttgttg
    10441 gttagaagcc ccgttactgc tcctacagtg cggggctttg ctttttgtgt tgattgtgtg
    10501 taatgtgtca ttacacataa atacattaca tgaggtgtaa ttaacgtgtc aacacacaaa
    10561 aacgaaaggc gtggtaatcc tccgttccag ttccgtttag atcctgactt acgggaaaag
    10621 atggaaaagg cacaacaaca ggacggcgat gattctcttg ctgcctggat aaagcgaata
    10681 atccgcaaag aattacagca acgcggcata gaacccaaag gataaaaact atcagcgccg
    10741 tggtgtgagg tactacggcg cattgcttta caggcgcaca caatgaccaa cgaagaatca
    10801 accgaaaaaa catcacctgc acgaaaaaga cgacgcagaa agaaagagca tgaatcagaa
    10861 cgattcgcgc cgtgttcttt tgcccttgag aagtacctta aagaatactc atcaaaggag
    10921 agaacttcgc agttatggca acgcactgaa ccagactgat tgcattgccc accagcctga
    10981 tagcggctat catccccgtg cttatgtttg ggatcacaca cacaaacggc gcagcgggtt
    11041 atctgttcag aaaggcggct ccatttcggg gccgcttttt ttatgcctga aaaaccccaa
    11101 ttttgtggtt ttccagtttc accagggcga acgaatcccc gcccacgttc tggcgtatat
    11161 tcaatcttca tggttatagc tctgtgttca gatgattgat gtgtggcggc tgcgtgccgc
    11221 cagcgtgatt aatgaactgc cttgcagcta tccttccagg ccagaacctc ggataaagac
    11281 cagccaacgg aacgaccgcc aagtttacga cgtgatggga attgtccggc cttttccagg
    11341 cggtagcggc atgagcggct aaggcctgtt agcttttcgc attctttttc acgtataaac
    11401 cgatcagtgc ttaacactat tgcccccttt cgtttcttaa agagttattt cgtgttctat
    11461 tgcgttggga tgtgtctgat tgtgtcagga tgattcagag ttggcaaatg ttgaggccgt
    11521 atggtttaca gaaagaggaa caatcaggat aaaatcattt aaattcatgt taatacaaag
    11581 gcataaaata ttgtttcatg ccttttttct cgctatttaa agagtgattc gctagtgtat
    11641 aaaaaaccag taacacgtta aaaatcagct acttataaat ccgtacactt tttcacctct
    11701 tgttcgtagt tgttccgcat tgttgctcat tgttgcacgt tgtatctgtt cgcatatcca
    11761 gtatgcgcat actgaaaaaa cacgaaaaaa attattttct tctggctact ggtagcgtgg
    11821 ttacgttttc atgtgttccc gccagtatcc ctaaccgctc cgtccacata tccagcgcat
    11881 tgcgtttagc atccagataa cgggaatgat tataaactcg ctgcattcct ggcatctggt
    11941 ggcctgtaag ctgctccacg acgtgaggat ccacgcctaa atcgttcagc atcgttgtaa
    12001 aggtgcgccg gatgtcatgt agtgaccagt gagggtgatt tagcctcctg tgcgctaatc
    12061 tgccgtactg cgacacgctt gtttcctgtt tcacttcccc cagcaataag cccgtgtgcc
    12121 tgttctgctc caccagctgc gtgacgaacg gcagtattgc ttccggtatg ggccggaata
    12181 ttgccacctt cgttttgctg tgttctttcg gcacggtcca gagcatttcg gtaaaatccc
    12241 actcgctgat ctccgataac ctcagttcta ccgtccggca tccgaacaca atcaggaggc
    12301 ggattaacgc gatgtagtag ggggagaata tttttttgtc cagtgcctgc aataattcgc
    12361 ccagttcttt ggtgcttaag acacgctcgc ttatatccgg ttttttccca acgtccgcca
    12421 cactcatatc atcaagaacg ttgctgattg catagcgccg cctccggcag aacttaagcg
    12481 cctgtttgca cgtctgtagc aagaatccgg cagtaacagg cgttcgcttt gccacctggt
    12541 caaaacaggc cagccagtgc cgtagctcgc atttatccag cggcatagca ccaatgtgct
    12601 gtattacgtg attattaagg cgctttttca gggcggcata atccacgcgg ttttcctttg
    12661 cgtacgactc aagccagtag gtgagcgcat cgccaaccgt taccggcttt aacgcttcct
    12721 gtacggtgta attaagctca tgacgtggat ttttcccctc tgccagccat gcgcgacact
    12781 gggcggcttt ttccctggct gatttcaggc tcagatcagg ataatttccc agcttaatgc
    12841 gttccggtgg tgcccccctt cccgttccgg ccctgtaagt gaaataccag gttaaaaggc
    12901 cactggttga atgcctgacg ctcaggtttc caccgtcatt aagaaaggct gttttttggg
    12961 cgggtgtgcc gttgattttc ctcagctgtg tatcgctcag tttgttaagt gctctgctca
    13021 taattttgat tccggtcatc acaattaact acaccattaa ctacaccgat cgttgcacaa
    13081 agggctacaa cgtgaaacaa gctggaacaa ggaaaatcac aaatctgttt ataatcaaaa
    13141 acataatgaa caatctgaaa cattatgaaa cggcaaaaaa cactaattga taaaatatgt
    13201 ccatacttca cgcattacgt taagcatccg ttataatcgg ttgcagatac cagcctgtgg
    13261 atgcttaaca tggaatacca actcactctt aactggcccg attttcttga acgtcactgg
    13321 cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt ttattgaccc gatctctcca
    13381 gacgagttgg caggtctggc gatggaaagc gaagttgaca gtcgactggt cagtcaccag
    13441 gatggcaaat ggcaggtcag ccacggcccg ttcgaaagct acgatcatct cggtgaaacc
    13501 aactggtcat tactggtaca ggcagtaaac cactggcatg agccgaccgc cgcgctgatg
    13561 cgaccgttcc gtgaactacc ggactggcgt attgatgatc tgatgatttc tttttctgta
    13621 cctggcggcg gcgtcggccc gcatctcgat cagtacgacg tgtttatcat tcagggtacc
    13681 ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga aacagcactg cccacatcca
    13741 gatctgttac aggtcgatcc gttcgaagcc atcatcgatg aagagctgga gcctggtgat
    13801 attctttata ttccgccagg attcccgcat gaaggctacg cgctggaaaa tgcgatgaac
    13861 tattccgtgg gttttcgcgc gccaaatacg cgggaattaa ttagcggatt tgccgattat
    13921 gtgctgcaac gtgaactggg cggcaactac tacagcgatc cggatgttcc acctcgcgct
    13981 catcctgcgg atgttctgcc gcaagagatg gataaactgc gtgagatgat gctcgaattg
    14041 atcaaccagc cggaacactt taagcaatgg tttggcgagt ttatatccca gtcacgtcat
    14101 gaactggata tcgcgccgcc ggaaccgcct tatcagccag atgaaatcta cgatgcactg
    14161 aaacaaggtg atgtgctggt gcgcctgggt ggtctgcgcg tattgcgcat tggcgacgac
    14221 gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc cggcactgga tgcactcgcc
    14281 agcaacattg cgctgactgc ggagaatttt ggcgatgcgc tggaagatcc gtcattcctc
    14341 gcgatgctcg cggcgctggt caatagcggg tattggttct tcgaagggta agtttggatt
    14401 taagccggat gcagcatcac atgccgcatc ccttttcgct tacttccgtt gcgccgttaa
    14461 ctcggcaata cggacgatca cctgcaccgc tttttccata ccttccagaa tcacaaactc
    14521 atgcttacca tgatagttgt aaccgccagt gaacaggttc gggcaaggta atcccataaa
    14581 cgacaactgc gcgccgttgg taccgccgcg gatcggtttc agttccggtt caatatcgca
    14641 gtcacgcatc gcctgctggg cgatatcgag aatatgcgga tgctcagcca ctttctcgcg
    14701 catattgtag taactgtctt caatcaccaa ttcaatgtag caatcaggat gtaacccttt
    14761 gcccactttt ttggcgatct ccatcatttt acgtttacgc gcttcaaact gtttacggtc
    14821 gaaatcacgg atgatgtagt gcatatcggc ccgttcaacg gtgcctttca tgctcgccag
    14881 gtgatagaaa ccttcatagc cttctgtcat ttccgggctt tcatccgccg gaacttccgc
    14941 atgaatacgt gccgccagcg acagcgcatt taccatcact cctttcgccg tgcccggatg
    15001 aacattgtta ccgacaattt tgatattgac cgatgcggcg ttgaagtttt caaactccag
    15061 ttcgcctacg ccaccaccgt caacagtgta agcccagcgg gcatcgaagg catcaacatc
    15121 aaaatgtttc gcccctttgc ccacttcttc atccggggta aaggcgacgc gaatatcacc
    15181 atgcggaatg tttttctgtt gcaataccgc cagcgcggtc atgatttctg caatacctgc
    15241 tttgtcatcg gcacctaaca aggttttacc atcggtggta atcaacgtct gacccagtag
    15301 ctgatgcagc accgggaaca taaccggtga taaaacttca tcgccgatac ccagcgcaat
    15361 atcgccaccg cgatagtttt caacaatttg cggattaaca tttttgccgc tgcaatccgg
    15421 tgaggtatcc acatgagaaa taaagccaat cgccgggata tcgccaggga cgttagccgg
    15481 taacgtcgcc atcaaagtgc ccttctcact taaggtcaca ttgataagcc ccatttcttc
    15541 gagctgctct ttcagcagat gcaataactt ccattggcct tccgtgctgg gaacctgtct
    15601 cacccctgct tttgattggg tatccagaga cacgtagttc aaaaatcgct caagtagttt
    15661 atccatgtag tcaccctcac tttttgtgac aacattatta agaagcaaga aaagacaaat
    15721 attgcgtcag gtcactttta cccctgcaag cgggaatatt tatcagcatt accttaatga
    15781 atataaagct aagccagtaa ttcacaacaa agattggcga ttcaagcggt ttgccgtaga
    15841 attaccgccc tcattaagag tcaccaaggt ggttaaccac aaaccccgca tcggtaagcc
    15901 atccgttgcg tttacatggg acagagtaaa aaattgaata aacaaccgag ttcgctttca
    15961 ccgctggtgc aattggcggg aattcgcaaa tgctttgatg gtaaagaggt cattccccag
    16021 ctggatctga ctatcaacaa tggcgagttc ctcacgctgc ttggcccttc tggctgcggt
    16081 aaaacaaccg ttcttcgcct gattgcaggt ctggaaactg ttgattccgg acgcatcatg
    16141 ctggataacg aggacatcac ccacgttccg gcggaaaacc gctatgtgaa cactgttttc
    16201 caaagctatg cacttttccc ccacatgacc gtgttcgaaa atgtggcctt tgggttgcgc
    16261 atgcaaaaaa cccccgctgc tgaaattacg ccccgcgtga tggaagccct gcggatggtg
    16321 cagctggaaa ccttcgctca acgtaaaccg catcagctct ctggtggtca acagcaacgc
    16381 gtcgccattg ctcgcgcggt ggttaacaag cctcgtctgt tgttgctgga tgagtcgctc
    16441 tcagcgctgg attacaaact gcgtaagcaa atgcagaacg agctgaaagc gttacagcgt
    16501 aagcttggca ttacattcgt tttcgtgact cacgaccagg aagaagcact caccatgtcg
    16561 gacaggattg tggtgatgcg cgatggtcgc attgagcaag acggcacgcc gcgtgaaatc
    16621 tacgaagagc cgaaaaacct gtttgttgcc gggttcattg gcgaaattaa tatgtttaac
    16681 gccacggtga tcgagcgact cgacgagcag cgcgtacgcg ccaacgttga aggccgcgaa
    16741 tgtaatatct acgttaactt cgccgttgaa ccggggcaaa aactgcatgt tctgctgcgc
    16801 ccggaagact tacgtgttga agagattaac gacgacaacc acgctgaagg gctgattggt
    16861 tacgttcgtg agcgtaacta caaaggcatg acgctggagt cggttgttga actggaaaat
    16921 ggcaagatgg tgatggtcag cgaattcttc aatgaagacg atcctgactt tgaccactct
    16981 ctcgaccaaa aaatggccat taattgggta gaaagctggg aggtcgtact ggctgatgaa
    17041 gaacacaagt aagttccaga atgtagtgat tgtcactatt gtcggttggc ttgtgttgtt
    17101 tgtctttctg cccaacctga tgatcattgg caccagcttt ttgacccgcg acgacgccag
    17161 tttcgtcaaa atggtcttta cgctggataa ctacacgcgt ctgctcgatc cgctctattt
    17221 tgaagtgcta ttgcactcgc tgaatatggc gctgatcgcc accctcgcct gcctggtgct
    17281 gggctatcca tttgcctggt ttctggcgaa gctgccacac aaggtgcgtc cgctgctgct
    17341 gtttctgctg attgttccgt tctggaccaa ctcattgatt cgtatctacg ggctgaaaat
    17401 tttcctcagc accaaaggct atctcaacga gtttttgctc tggctgggcg ttatcgacac
    17461 accaatccgt atcatgttca cgcccagtgc ggtgattatc ggtctggttt acattctgct
    17521 gccgtttatg gtgatgccgc tgtactccag tatcgaaaaa ctggataagc cgttgcttga
    17581 ggcggcgcgc gatctcggtg ccagcaagtt acagactttt atccgcatca ttattccact
    17641 gacgatgccg gggattattg ccggatgtct gctggtgatg ctgccagcga tgggcctgtt
    17701 ctatgtgtcc gacctgatgg gcggtgcgaa aaacctgctg atcggtaacg tcatcaaggt
    17761 acagttcctc aatattcgtg actggccgtt tggtgcagcc accagcatta cgctgactat
    17821 cgtaatgggc ctgatgttgc tggtttactg gcgcgcttct cgtttgctga ataagaaggt
    17881 ggaactcgaa tgatcggtcg actgcttcgc ggcggtttta tgaccgctat ctacgcgtac
    17941 ctgtatatcc caatcattat tttgattgtg aactccttta acagctcgcg ctttggcatc
    18001 aactggcagg gttttaccac caaatggtat agcctgctga tgaacaacga cagcctgtta
    18061 caggcagcgc agcattcact aacaatggcg gtgttttcgg cgacgtttgc tacgcttatc
    18121 ggttcactga cggcagttgc gctgtaccgt tatcgctttc gcggtaagcc gttcgttagc
    18181 ggaatgctgt ttgtggtaat gatgtcacca gatatcgtga tggcaatttc gctgctggtg
    18241 ctgtttatgc tgctgggtat tcagcttggc ttctggtcgc tgctgttctc gcatatcacc
    18301 ttctgcctgc catttgtggt ggtgacggtg tattcgcgcc tgaaaggttt tgacgtgcgg
    18361 atgctggaag cggcgaaaga tctcggtgcc agcgaattta ccattctgcg aaaaatcatt
    18421 ctgccactgg caatgccagc ggtggcggcg ggctgggtgt taagctttac cctgtcgatg
    18481 gacgatgtgg tagtttcttc attcgtcacc ggaccaagtt atgaaattct gccgttaaaa
    18541 atttattcga tggtcaaagt cggcgtatcg ccggaagtta acgcgctggc aaccatatta
    18601 ctggtgctgt cgctggtgat ggttattgcc agccagctta ttgctcgtga taaaacgaaa
    18661 ggtaacacag gggacgttaa atgaaaaaat ggtcacgcca cctgctcgcg gcgggtgctc
    18721 tggcactggg catgagcgcc gctcacgccg atgacaacaa cacgctgtat ttctacaact
    18781 ggaccgagta cgtgccgcca ggactgcttg aacagttcac caaagaaacc ggtattaagg
    18841 ttatctattc gacttacgag tcgaacgaaa ccatgtacgc caagctgaaa acgtacaaag
    18901 acggtgccta tgacctggtg gttccttcaa cctattacgt cgataaaatg cgtaaagagg
    18961 ggatgatcca gaagatcgac aagtcgaagt taacgaactt cagcaatctc gatccagaca
    19021 tgctcaacaa gccttttgac ccgaataacg actactccat tccgtatatc tggggtgcga
    19081 cggcgattgg tgttaacggt gatgcagtgg atccaaaatc tgtcaccagc tgggccgatc
    19141 tgtggaagcc agagtacaaa ggcagccagc tgttgaccga cgatgcccgt gaagtgttcc
    19201 agatggcgct gcgtaagctg ggctactccg gtaacaccac cgatccgaaa gagattgaag
    19261 ctgcatataa cgagctgaaa aaactgatgc caaacgtcgc agcgtttaac tccgataacc
    19321 cggctaaccc gtacatggaa ggcgaagtta acctcggcat gatctggaac ggttctgctt
    19381 tcgttgcacg ccaggcgggt acgcctattg acgtggtgtg gccgaaagaa ggcggcatat
    19441 tctggatgga cagcctggcg atcccggcaa atgccaaaaa caaagaaggc gcgctgaaat
    19501 tgatcaactt cctgctgcgc ccggatgtgg caaaacaggt tgctgaaact atcggttatc
    19561 caacgccaaa ccttgcggcg cgtaagctgt taagtccaga agtggcaaac gataagacgc
    19621 tctacccgga tgctgaaacc attaaaaatg gcgaatggca gaatgacgtt ggcgcagcca
    19681 gcagcattta tgaagagtat tatcagaagc tgaaagcagg acgttaattc agcaagcggg
    19741 cggtggtatt accgcccgtt ttttagttag caaggatgtg gctaatgagt caggacagta
    19801 aagttttatc ccgctctttc gtcggtattg tggtccgctt gtaggcctga taagcgtagc
    19861 gcatcaggca atgcttcctg ctttataatc cctccagcaa cttttcaaca aattctggca
    19921 ccacctggct tgccgggccg taatatttct cggcaaattc attaccaacc tgactcggtt
    19981 caagattcag ttcaacggtg tgagcaccat gtagtttggc ttcgtgtaca aacccagcag
    20041 ccggatagac atgcccggaa gtaccaatgg caataaagac atccgccatc gacaacgcca
    20101 tataaatttc atccatgccg agtggcattt cgccaaacca aacgacgtgc ggacgcaagg
    20161 gtgccgggaa ctggcaacaa tggcatttat cttctggcgt aacgtctccg gtccagtcga
    20221 gaacctgacc actttgcgaa caacgaactt tcagcaattc cccatgcata tgaatgacat
    20281 tggtattacc tgcgcgttca tgcaggttgt ctatattctg cgttaccagc aaaaagcgat
    20341 cgccgagagc gtcttgcagt ttagccagcg caagatgcgc ggcgttaggc tgaatttctg
    20401 gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg caccagttca ggatcgcgat
    20461 cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc ttcccacagg ccatctgcgg
    20521 cgcgaaaggt acgaatacct gattccgcag aaattcctgc ccctgtcagt acgagtactc
    20581 ttggtttttc cattgcttcc ggcaccactt tatctctgaa aaaaatacgc tgacgcaaac
    20641 gctcgcgcag gcgacgttta tttttacgaa aacgacttaa ccgatgaccc cgacgcgaca
    20701 gcatagcaac ctctgttgtt taatcggtta gatgtaggaa ggccgcacca cgcattcctc
    20761 ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt aagagatgac
    20821 aaggcaacct gtccgccagt tgcgttgtga ttgccgggaa attcgataag ccaccaccaa
    20881 tgacgaccag gtcagggtca acaatggtca ggatatttcc cagagaaacc gctaataaat
    20941 ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc ttgatcataa agcgcaataa
    21001 tttcgggagc ctgcaacggt tgatgataat agtgttgata cagccacgca aaaccgcgac
    21061 cagacagata attttcaatg cagccatgct gaccacagcc gcagcggcgt aacgggaaat
    21121 ccaaccccat catggttaac gcatcaaccg gcagacgcat atggccaaac tcgccggtaa
    21181 tgtagctttt accagtaatc ggtttgccgt tgaaaaccag cccgccgcca acgccggtgc
    21241 cgagaattaa ccccatcacc agtggatatt gcgtaaattc atcctcccag gcttctgaaa
    21301 gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg atcaagacgt gcgctcaggt
    21361 cggcacgcag cggtttgccg ctggcagcgg ggacattagc ggcatacagc ttaccatctt
    21421 ctgtttccgg cattcccgga ataccgatgc cgacagaacc tttacagcca aaacgttggt
    21481 cagcttcagc taccagctca cacactgcat ctaaaaatgc gtcatagctg tcacgcggtg
    21541 tcggcacccg cttttcccac tgcaactgcc gaccgctatc aaacacgcca agcgcaattt
    21601 ttgttccacc aatatcaaac ccgtaataca ttgccgctcc ttgttttaat gtactgcctt
    21661 tactggccgc taaggactcg cgcagggtca atattactgg cgcgccgcgc cggataccaa
    21721 cttgccaaaa gactcagcaa caatgctgtg accagtacgt agaagacgtc cagccagtgc
    21781 aattccgatg gcaggaagtc aataaaatag atatcgctgg agaggaactg atgaccgatc
    21841 agcttttcaa tccactcaat aatcggggta agttgcagtg aaacaactac gccgataacc
    21901 accccacaca ggctgccgaa cagccctgcc agcaatccat accagacaaa gatggcgcga
    21961 attaaaccat ctttcgcccc cagcgttctt aatactgcga tatcgccact cttgtctttc
    22021 accgccatca ctaaggtgga gacgatgttg aaacaggcca cgccaatcac cagtaccatc
    22081 gccagataca taatggcgcg gatcatctgg atatcgcgat acatatagcc gtaagtacca
    22141 atccaacttt taatataaac atagctgttg gtcacttcac ccgcatcgcg taccagctta
    22201 ttggcgttga aaacatccgt cattttaagg gcaatacctg acacgctgga acccatatca
    22261 agatattgtt gggcatccgc cagcgggatc atggcaaaac tgtgatcgag ttgaccactc
    22321 aactgcaaaa taccggcaat gtgcaaacgc acacgttttg gctgcatcag tttatgctcg
    22381 ggattcgagt tggggatcat gatcgacacc caatcgccct gcttcacttt cagcgcatcc
    22441 gccacgcctt tgccgatgat aatttgctgt tcgcccgctt taaaattgcg ccacgcatcc
    22501 ccctgaacaa acgagggtaa tgcgctcaga cgctgttcct gttgtgggtt aacgcccttc
    22561 acctggattg cgcgcagatt cgctccactt tccaccagac cggtgaaatt gatatacggc
    22621 gcagcggcgg caatacccgg cactttctgc acgttatcca gtgcttcctg ccagttagtc
    22681 catggctgat ttaccgcttc gatttcgcca tgcggcacca ccgccagaat gcggttattc
    22741 agttcgcgtt caaagccgtt catcgcgctt aagccgacga tcaataccgc cacgccgagg
    22801 gcaatgccaa tggtagaaat gacagagatt agcgacacca tgccgccgcg tcgccgtccg
    22861 cgactaaaac gcaggccaat caataacgat aaaggcatcg ccattactcc gcccccatca
    22921 ggctcagttc cgccgtcaga cgtccatcac gcatttccag ttggcggctc atacgtttcg
    22981 ccagttgcag gtcgtgagtg accaccagga aagcggtgcc ctgcaagcga ttcaactctc
    23041 caagcaactg gaagatgctg tcggcgttac gcgcatcgag gttaccggta ggttcatccg
    23101 ccagtaccag acgcggatta ttgaccagcg cacgggcaat cgctacacgc tggcgttcgc
    23161 cgccagaaag ttcagatggg cggtgattcg cacgatgctc cagccccacc gcttttaaca
    23221 tctccagtgc acggctgttg atttcagcgg gctttttctt gccaatcagc agtggcatag
    23281 ccacgttttc cagggcagta aaatccggca gcaggtggtg aaactgataa ataaagccca
    23341 gcttctggtt gcgcagttct gctttcgccg ctgaagacag tttgctcatc ggctgaccgt
    23401 taaagatcac atcgccggag gttggcgtat ccagcccgcc taacaggtgc agcaaggtac
    23461 ttttaccgga accagagcta ccgacaattg ccatcatttc accttcgccc acgctgaaac
    23521 tgacattgtg cagcacatcg gtttgcacac tgccttcctg atagcgtttg cacaggttgt
    23581 cgcattgcaa caggatctta ttcataacgt aaagcctcag cgggttgtgt ggcggcagcg
    23641 cgccatgaag ggtaaagcgt agacagcagc gcgatagcca tcgccaccag cgcaataaca
    23701 atgacctgta aaggttcgat agccaccggc agcgccgcgc catcaagcag gacgccgatt
    23761 atcggcatca gattatttaa ctggctggca agcagtgcgc caagcgccgc tccgaggatt
    23821 gcaccgataa tcccggcgct ggccccttgc accataaaga ccatcatgat ttgtcgcgga
    23881 gttaagcctt gcgtttgcag gatcgcgact tcgccctgct tctccatcac catcagcccc
    23941 agcgaggtaa taatgttaaa cgccgcaaca gcgacaatca ggctcagcag taagcccatc
    24001 atattttttt ccatgcgtac ggcctggaac agctcgcctt tacggtcacg ccagtcctgc
    24061 catttgctgc cttcaggcag tttttgctga cttaaagagt caactttcag cggctcatcc
    24121 agccacaaac gccagccggt aatattggct gccggataac gcatcagacg cgaggcatcc
    24181 tcaatattca ccagcatttc atagccatcg acttcactgt tggcggcgaa agtgccaatc
    24241 acattgaaca ggcgctgact tggaatacgc cccatcggcg tgaactggct ggcagatggc
    24301 accatcacgc ggatttgatc accgcgattg acgcccaact gtgaggcaag ttgttcgccg
    24361 aggatgacat tatatttccc cggctcgagg tcagtttgtt tcacattgac cagatacggc
    24421 gttagtggat ctttttgcgc cgggtcgata ccgagcatca ccccgaccgc cacgctgcgc
    24481 gcgctttgca gtaccacatc accggtagta ataggtgcga cgcgattaac gccgtccagt
    24541 ttgactgccg tttccgggag ttgctgcggg ttaagagagc catgctcaga agagagaatt
    24601 gcctgtggca tcaggccaag gatgttgttt tgcagctcgc gctcaaagcc gttcatcact
    24661 gacaatactg tgaccagcgc catcaccccg agggtaatgc cgatggtaga aagccaggag
    24721 acgaaacgac cgaagcgatc cgctgcacgc ccacgcatgt aacgcaagcc aataaataga
    24781 gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc aaagacacga gtatataagc
    24841 gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt cagggctaat ctgaaaagcc
    24901 cccgtaaatc aatcggctaa attgccatgt tttatcatca atcttgccgc ctgatcttaa
    24961 tctgaaaaat catccaatac agacagatac ccattacacc tcgggtcata accagcagga
    25021 tcaaccgcat gtaaattaaa aaagagcgtg ttgttctgat gaaacaaaaa gagctatgga
    25081 ttaaccagat caaagggtta tgtatttgtc tggtggtgat ttatcactcg gtcattacct
    25141 tttatccgca tatgaccact tttcagcatc cgttatcgga agtcctgagc aaatgctgga
    25201 tctatttcaa tctttacctt gccccctttc gtatgccggt ttttttcttt atatccggct
    25261 atttgattcg ccgctatatc gacagcgtac cgtggggaaa ttgtctcgat aaacggatct
    25321 ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca gtggctggca cctgagcgcg
    25381 atttaagcaa tgcctccaat gccgcttatg ccgattccac cggtgagttc ctgcacggga
    25441 tgatcaccgc cagcaccagc ttgtggtatc tgtatgcgtt aattgtctat ttcgtggtat
    25501 gtaaaatttt tagccgtctg gcgctgccac tattcgcctt gtttgtactg ctgagtgtgg
    25561 cggttaattt cgttcccacg ccgtggtggg gaatgaacag tgtgatacgc aatttgcctt
    25621 attacagcct tggcgcatgg tttggcgcaa caataatgac ctgtgttaaa gaggtgccgt
    25681 tgcgccgcca tctgctgatg gcttctttgc ttaccgttct ggcggtcggt gcctggttgt
    25741 ttactatctc gctgctgttg tcgctggtat cgactgtggt gatcatgaag ctgttttatc
    25801 agtacgagca acgattcggt atgcgctcca ccagcctgct gaatgtgatt ggctccaaca
    25861 ccattgctat ctacaccacc catcgcattc tggttgaaat attcagctta actctgcttg
    25921 cgcaaatgaa cgcagcacgc tggtcgccgc aagtcgaact gacactcctg ctggtttatc
    25981 cctttgtcag tttgttcatc tgtactgttg cgggcttgct ggtaagaaaa ctttcacagc
    26041 gtgcattcag cgatctgttg ttctccccgc cttcactgcc cgctgccgtc agttactctc
    26101 gttaaaccat caccccagcc ggggcgattc aatttgctaa accatgtcga ttacggataa
    26161 tgaacagcat tgcttatcag agatatgccc ccatatgttg aggcatatcc taacgagaat
    26221 ctgacaaccg ttatgcctga acaatatcgt tatacgctgc ccgtcaaagc gggtgagcag
    26281 cgtctgctgg gcgagttaac cggcgcagcc tgtgcaacgc tggtagcgga aattgccgaa
    26341 cgtcacgccg gtccggtggt actcattgca ccagatatgc aaaatgctct gcgtttgcat
    26401 gatgaaatca gccagttcac cgatcagatg gtgatgaatc tggcggactg ggaaactctt
    26461 ccctacgaca gtttttcgcc tcatcaggac attatctcct cgcgcctttc caccctttac
    26521 cagctaccga cgatgcagcg tggcgtactg attgttccgg tgaatacgct tatgcagcgc
    26581 gtttgcccac acagttttct ccacggtcat gcgctggtga tgaaaaaagg tcagcgcctg
    26641 tcacgagatg cattacgaac ccaactggac agcgccggtt atcgctatgt tgaccaggtg
    26701 atggagcacg gcgaatacgc cacgcgcggc gcgttgctgg atctcttccc gatggggagt
    26761 gagctgcctt atcgtcttga tttctttgat gatgaaatcg acagcctgcg ggtgtttgac
    26821 gtcgacagcc agcgcacgct ggaggaagta gaagcgatca atctgctgcc cgcacacgaa
    26881 tttccgaccg ataaagcggc aattgaactg ttccgcagcc agtggcgcga taccttcgaa
    26941 gtgaagcgcg atccggaaca tatttaccag caagtgagta aaggcacatt acctgccggg
    27001 atcgagtact ggcagccatt gttcttcagc gaaccactgc cgccgctgtt cagttatttc
    27061 cctgccaata ccttgctcgt gaatactggc gatctggaaa ccagtgccga acgtttccag
    27121 gctgacacgc tggcgcgttt tgagaatcgc ggcgtcgatc cgatgcgccc gctgttgcca
    27181 ccacaatcgc tctggctgcg ggtggacgag ctcttctcag agctgaaaaa ctggccccga
    27241 gtgcagctaa aaactgaaca tttaccgaca aaagccgcga atgccaattt aggtttccag
    27301 aaactgccag acctggccgt tcaggcgcaa caaaaagcgc cgctggatgc gctgcgtaag
    27361 ttcctcgaga ctttcgacgg tccggtggtg ttctcggtag aaagtgaagg tcgccgtgaa
    27421 gcgctgggtg aactgctcgc acgaattaaa attgctccgc aacgcattat gcgtcttgat
    27481 gaagccagcg accgtgggcg ttatctgatg attggcgctg ccgaacatgg ttttgtcgat
    27541 aaggtgcgta atctggcgct gatctgcgaa agcgatctgc tcagtgaacg tgttgcccgc
    27601 cgtcgtcagg attctcgccg caccatcaac cccgatacac tgatccgtaa ccttgcggag
    27661 ctgcatattg gtcagccggt ggtccatctg gagcacggcg tcggtcgcta tgccggaatg
    27721 accacgctgg aagctggcgg cattactggc gagtacctga tgctcaccta tgccaacgac
    27781 gccaaactgt atgttccggt gtcgtcactg catctgatta gccgttacgc aggtggcgcg
    27841 gaagaaaacg ccccgctgca taaacttggc ggcgatgcgt ggtcacgcgc gcggcagaaa
    27901 gcggcggaaa aagtgcgtga tgtggcggcg gaattgctgg atatctacgc gcaacgcgcc
    27961 gccaaagagg gcttcgcgtt taaacacgat cgtgagcagt atcagttgtt ctgcgacagc
    28021 ttcccgtttg aaaccacgcc ggatcaggcg caggccatta atgcggtact taacgacatg
    28081 tgtcagccgc tggcaatgga tcgtctggtg tgcggcgatg ttggctttgg taaaacagaa
    28141 gtggcgatgc gcgccgcttt cctggcagta gataaccaca agcaggtagc ggtgttggtg
    28201 cctaccaccc ttcttgcgca gcagcattac gacaacttcc gcgaccgttt cgccaactgg
    28261 ccggtacgta tcgaaatgat ctcccgtttc cgcagcgcca aagagcagac gcaaatcctt
    28321 gcggaagtgg cggaagggaa aatcgatatt ctgatcggta cgcacaaact gctgcaaagt
    28381 gacgtcaagt ttaaagattt aggcctgctg attgtcgatg aagaacaccg cttcggggtg
    28441 cgtcataaag agcgcattaa agcgatgcgc gcgaacgtgg atattctgac gcttactgca
    28501 acgccgatcc cacgtacgct gaatatggca atgagcggaa tgcgtgacct gtcgattatc
    28561 gccacgccgc ccgcccgtcg tctggcagtt aaaacctttg tccgtgagta tgacagcctg
    28621 gtggtccggg aggcgatcct gcgtgaaatt ttgcgcggag gacaggttta ttatctctac
    28681 aatgatgtgg aaaacattca gaaagccgcc gaacggctgg cagaactggt gcctgaagca
    28741 cggattgcca tcggtcacgg gcagatgcgc gagcgcgaac tggaacgggt gatgaatgat
    28801 ttccatcatc aacgtttcaa cgtgctggtt tgtaccacca ttatcgaaac cgggatcgac
    28861 atcccgacag ccaacaccat tatcattgaa cgtgcggatc acttcggtct ggcgcagctg
    28921 caccagttac gcggtcgcgt cggacgttca catcatcagg catatgcatg gctgctgacg
    28981 ccgcatccaa aagcgatgac taccgatgca caaaaacgtc ttgaagcgat tgcctcgctg
    29041 gaagatctcg gggcaggttt tgcgctggca acgcacgatc tggagattcg cggcgcgggt
    29101 gaactgcttg gcgaagaaca aagcggctca atggaaacca tcggtttctc gctgtatatg
    29161 gagttgctgg aaaacgccgt cgatgcactg aaagccggac gcgagccgtc gctggaagat
    29221 ctcaccagcc agcaaacaga agtcgagctg cggatgccgt cgctattgcc agatgatttc
    29281 atccctgacg tgaacacgcg tctgtcgttc tacaaacgta ttgccagcgc caaaacggaa
    29341 aacgaactgg aagagatcaa agtcgagctt atcgatcgct tcggcctgct gccagatccg
    29401 gcgcgtaccc tgctggatat tgcccgtctg cgccagcaag cgcagaaact ggggatcagg
    29461 aagctggaag gtaatgagaa aggcgggatg atcgaatttg ccgagaagaa tcacgttaat
    29521 ccggcctggt tgattggttt gctgcaaaaa cagccgcagc attaccgcct tgatggtccg
    29581 acgcgcctga aatttattca ggatttgagt gagcggaaaa cgcgtatcga atgggtacgc
    29641 cagtttatgc gtgaactgga agagaacgcg atcgcttaat gtaaatcggg ggcgtttctg
    29701 cgcccccatc tgcaacattt acaaattctt tgcactttcc tgcactatcc gacactgtca
    29761 ccatccataa ttcaggctta tctgtttatt acaataacct tatatttatt atggattttt
    29821 ggtgatgatc aaaacgcgtt tttctcgctg gctaacgttt tttacgttcg ccgctgccgt
    29881 ggcgctggcg ctaccggcaa aagccaacac ctggccgctg ccgccagcga gcagtcgtct
    29941 ggttggcgaa aacaaatttc atgtggtgga aaatgacggt ggttctctgg aagccatcgc
    30001 caaaaaatat aacgtcggct ttctcgctct gttacaggct aaccccggcg ttgatcctta
    30061 cgtaccgcgc gcgggcagcg tgttaacgat cccgttgcca accctacttc cagacgcgcc
    30121 gcgcgaaggc attgtgatca acattgcgga gctgcgtctc tattactacc cgccgggtaa
    30181 aaattcggta accgtgtatc caataggtat tggtcagtta ggtggtgaca cgctgacacc
    30241 gacaatggtg accaccgttt cagacaaacg tgcaaaccca acctggacgc caacggcaaa
    30301 catccgcgcc cgttataaag cacagggaat tgagttgcct gcggtagtgc cggctggacc
    30361 ggataaccca atgggccatc atgcgattcg tctggcggcc tatggcggcg tttatttgct
    30421 tcacggtacg aacgccgatt tcggcattgg catgcgggta agttctggct gtattcgtct
    30481 gcgggatgac gatatcaaaa cactctttag ctaggtcacc ccaggcacca aagtgaatat
    30541 catcaacact ccgataaaag tctctgccga accaaacggt gcgcgtctgg ttgaagtaca
    30601 tcagccgctg tcagagaaga ttgatgacga tccgcagctg ctgccaatta cgctgaatag
    30661 cgcaatgcaa tcatttaaag atgcagcaca aactgacgct gaagtgatgc aacatgtgat
    30721 ggatgtccgt tccgggatgc cggtggatgt ccgccgtcat caagtgagcc cacaaactct
    30781 gtaaatcctg gataaaaaaa atccccgcga cacggcatgt cgcggggaaa aaatcagcag
    30841 tagcaggcat taatgagggt taatgcttat ttataaatta ctgctgttcc atggagggta
    30901 ttcggaccgg ttacagaagt aatacggaaa gattttgcgc ccatctcatc cgctttttgc
    30961 gccagctgct cttccagcga tcccagattt gtccccgcgt tagcactgat agtaccgact
    31021 ttttgttggc cttctggcgt tgactgaact tcgacagccg caaagttggc aaatgacatg
    31081 gagcttaaaa tcgccgcagc gatgagggtt tttacgtttt tcataatagt ggccttatgc
    31141 agatgaatga cgtcgcaaga aattacttaa cgatcgataa ctaaatgata gatgtgatct
    31201 ggatcacata caagatattt tttataacaa tcattaatta atttaaaaat ctttaaattt
    31261 cagtcaaata gaaagcaatt atttttatgg aatctggact ggtgaacgga ggtgattatt
    31321 tttataataa cctttcattc acagtcacca ggtacaacgg catcatggca actgactcaa
    31381 cacaatgtgt aaaaaaaagc cgtggccgcc caaaagtgtt cgacagggat gccgcgcttg
    31441 ataaggccat gaaattgttc tggcaacacg gttatgaagc gacttctctt gcggacctcg
    31501 tcgaagcgac cggagccaaa gcgcccacgc tatacgcgga atttaccaac aaagaggggt
    31561 tatttcgtgc cgttctcgac cgctatatcg atcgttttgc cgctaagcat gaagcacagc
    31621 tgttttgtga agagaaaagc gtggagtctg cgctggctga ctattttgct gccatcgcca
    31681 actgctttac cagcaaagac accccggcag gctgcttcat gatcaacaac tgcaccaccc
    31741 tctccccaga ttcaggagat atcgccaata cgttgaaatc acgccatgcg atgcaagagc
    31801 gcactttgca gcagttttta tgtcaacgac aagcgcgcgg ggaaatcccg acccactgtg
    31861 acgtgacaca tctggcagaa ttccttaatt gtattattca ggggatgtcg atcagcgcac
    31921 gcgaaggtgc atcgctggaa aaactgatgc agattgccgg aacgactttg cgtttatggc
    31981 ccgaactggt gaaataagtc gcaaaaaaag cccctcagcc tgagcgaatg aggggccaaa
    32041 ttgcagagta cagccttgtt atactttgtt attcaaaatc agttgcccgt tactatctag
    32101 cgggatctgc gtacccggat cgcggtccat gcggattttg ccctgctgat cgccaatctt
    32161 ataggtcaca tcataaccga gcattttttc tgacttgtca tacaccgttt tacaacgctg
    32221 ttgcgtagtc gtgtaagtat cgctttcctg gagagagccc tggatctggt taccggcata
    32281 tccaccaccc agcgccccca caacagtggc gacatcttta ccgcgaccac caccaaactg
    32341 atgccctatc acgccaccag caacagcgcc gagcaccgac ccggtaatgc gattttcatc
    32401 ctgcaccggt cgacgatggg tcactgtgac gttgcgacac tcctgacgcg gtgttttaac
    32461 cgtttccttg attggggttg cagaaacaac ctgagcgtat tgcgggcccc gttcaaacac
    32521 gttcagactg gccactgccg ctacgcccaa cgcagctgcg acaccaatcc cgatacccgc
    32581 caacattgat ttattcacgg gaacacctcc ttctttgcag ttatgccaat catgcaagcg
    32641 aaggagaaaa gcagctatca gacagaagat gaaaagcgcg ggaatgactg gaaatactta
    32701 aggattcgga ggagtctgaa ggaatgcctg atgcgacgct taacgcgtct tatcaggcct
    32761 accgctcgta ccatttgtcc agggcggata aggcgtttat gccacatcag ccagtgtacg
    32821 tcgattaatg caacttcaaa cgcggacgga taacgcggtt aatactcccc accagcatca
    32881 ttaatccggt tttaaagtaa ccatgcagcg caatctgatg cattcggtat agcgagatat
    32941 atacaaagcg cgcaattcgt ccttcaatca tcattgagcc gcgcgtcagg ttacccatca
    33001 ggctaccaac ggtggagaag ttcgacagcg ataccagcga accatgatct ttatactgat
    33061 aatttttcag cggcttaccg ttcatctgcg ccagaatgtt gttcattgcg caagtcgcca
    33121 tctggtgtgc agcctgagca cgcggcggaa caaagccccc ttccggacgc gggcatgacg
    33181 cgcagtcgcc aatagcgtaa atgtctggat cgcgagtggt ttgcagcgtc ggttccacca
    33241 ccagctggtt gatacggtta gtttcaagac caccgatatc tttcaggaag tctggcgctt
    33301 tgatcccggc tgcccatacc atcagatcag cctcaatata ttcgccatct ttagtgtgca
    33361 ggccgccctc atcagcactg gtgaccatgg tttgcgtcag cacgcgaacg ccaagtttcg
    33421 ttagctcgtt gtgggccgca gcagagatac gtggcggtaa tgcaggcaaa atacgttctc
    33481 ccgcttctac cagcgttacg ttcagggctt cgttggtcag gcctttgtaa ccgtagctgt
    33541 gcagttgctt gatcgcgttg tgcaattcag cggagagttc tacacccgtc gcgccgccgc
    33601 cgacaatcgc aatgttcact ttgccatttg cgcccaggtt ggcagagtat ttcaggaaca
    33661 gattcagcat ctcctggtgg aagcgacgcg cctggtgcgg gttatcgagg aaaatgcagt
    33721 tctctttgac gcctggcgta ttgaaatcgt tagaggtgct acccagcgcc atgaccaggg
    33781 tgtcataggc gattttacgt tccggaacca acagttcacc tttctcgtcg cgtagttctg
    33841 caatagtgat tgttttcgct tcacgatcaa tatcaatgac ggaacccaac tggaactgga
    33901 aaccatgatt gcgcgcatgg gccagatagc tcaacgcatc gacgccttca tcaagcgagc
    33961 cagtcgccac ttcgtgcagc agtggtttcc acaggtggct gtggttacga tcgaccagcg
    34021 taattttggc ttttttctta cgtcccagct tatgccccag ctgtgttgcc atttccagcc
    34081 caccagcacc gccgccgaca atcacaatct ttttcaatgg cgtagtcaac gtgaccccct
    34141 taaatttatt aaccaattgt taattaatgg ttattaacat agctcttaat taacaacagg
    34201 ttacagcaat gaaaatgttc ttcgctattg agaataacat gaatggtgca ttggtcatac
    34261 caaaattgat gttaatcaag ttttgttgct taaaagatag gcaactttac cgggggagat
    34321 caaaaaatag cgtttattat ttgagcaaat tcggaagagt aatatagggt gaagcgtata
    34381 tcgagggcgt ttggtcgtcc tgataagatg cgcagcatcg catcaggcat tgatgctcga
    34441 ctgtcggatg cggcgtggac gccttatccg acttacagaa ttcggagcgc atcaggtacc
    34501 ggcaaggtta cctgatgcga cgagggcatt tacccgaggg ttttgaacgc tttaatgcgc
    34561 tgtaaatgcg gggagatatt cttgaatttg tgcgtctgct cttcgtccca gacaatctcg
    34621 taataatgat gcaactcttc agatgtccgc tggctgttaa gtgcttcatc attacgcgac
    34681 aaaataacca gacaacgatc gcgattcttc tcgcggaagt tggtcacaca cttagtggca
    34741 atatcggcat actcttccgg gcgatcaatc ttgccttcca tgttctcata agggaacaaa
    34801 ttagggttga agatcacctg gcggatatcg caaagaaaac caatccgttc cgcccagtat
    34861 ccgcccaggc caacgccgca aattagcgga cgctcgtcaa cgttcagttg caacattttg
    34921 tccacttctt taagcagatg ctgcatatca tgtttcggat gccgcgtact gtagcttatc
    34981 aagcgtacat ccgggtcaat aaactgcaat tgtaagactt tctcgtggtt acccggactg
    35041 ttagagtcaa aaccgtgtaa atagataatc atcgcttcct cacataagcc agggttagtg
    35101 acctgctttc tcttcctgcc agcgttcatg taactgattc agacgggtgc tgatcgcttt
    35161 ccagcgagcc gagtccatca gttcctgtcg cgaaaatgaa cctttatgat acaaacgtgt
    35221 aacacgttct gccttgatcg gtgacagatt atctaacacg ctgacggccc ctttacgatt
    35281 attgcagacc aggatcatat cgcaacccgc atccagtgat gcctgcccgc gttcggcata
    35341 actgcccata atcgcggcac cttccatcga taaatcgtca gagaaaatca cgccgtcaaa
    35401 acccagttcc tgacgcaaaa cggttttcag ccagtaggga gaaccgctcg ccggacgcgg
    35461 atcaacatca ctgtagatca catgcgcagg cataatggcg tcgagtttat tttcgcggat
    35521 taaggaactg aagaccgaca tatctttagc gcgaatctcc gcttgtggac gtggatcgca
    35581 cggtgtttct ttgtgtgagt ctgccgttac tgcaccgtgt cctgggaagt gtttcccggt
    35641 cgttttcatt ccggcttcat gcataccatc aataaaccgg ctggcaattg ccagggcttt
    35701 ttgtggatcg gcatgataag aacgctcgcc aatcgccgcg ctgatatgcc cgacatccag
    35761 cactggcgca aagctgatat caatatccat agcgatcatt tcgctggcca tcagccaacc
    35821 cgcctcttgc gccagtttgc cgccctcttc cattcccaac agcgcagcaa aggattgtgc
    35881 tgccggtaag cgggtaaaac cttcgcgaaa acgctgcacg cgtccacctt cctgatctac
    35941 cgccaccacc agatgattgc gcgatgctgc gcggatctgg cgcaccagtt cacgtaactg
    36001 ggcaggatca tgatagttac gcgtaaagag aatcagccct cccaccagcg gatgcgccag
    36061 tatttcacgc tcttccgcgt ccagttcgta accttcgaca tccaacatta ctggacccac
    36121 actgctctcc ttattgtttt attaatagct gccgccaggt gtcatcggcc agcctgataa
    36181 attgttgatc gccggtttgt cgccagcggt actcaaacca ccctcctttg agcatcagca
    36241 gccagggaaa ccatcgcctg acctgacgcc ataattgcgc cggataaatc ttcgcgcgag
    36301 tggcatagtc attgaccaat tgccggtgct gttcagtatt ttccacccac accgccgcca
    36361 gttccagcgc gatatcacca tctccagcat actcccagtc gatgagtttt aaccctgatg
    36421 cgctatgcac taaatttccg gcgtggacat ccatatgcaa tggacttaag cgtaaaggtc
    36481 gtggttcccg cgctttgcgc agacgtttta acattcgcag ccaacccaca gtccgccgcg
    36541 ccggatcgct ttgctgccag tacagttcca gtaagggcaa cagcgttatt cgccagccaa
    36601 aacgtggttg ttgatgtaga taatacagca agcctgccag ttcgttggta tccggcaaat
    36661 acgtttttac cgcgccgggc agatagtcga ctaccatcca gtcacggaga tataaatgcg
    36721 gcttcggtgc aatgcttgcg ggtagttgtg ataaagcccg atactggcgc aagaacgcgg
    36781 actgcggcgc atcaggatcg tgcggctgac gcacaacaaa acgctggccc tgatgttcaa
    36841 tgagaaaact accgccacta agcccactat taaacgtcgt gacgggatga aactgcggga
    36901 aaaagcgcga cagcaattcg tcgcgcgtga tgggattatt gctgcgaaac ggcaccttta
    36961 cctgaccaga taatttcgcc cgtctgcacc agcatcagct gcatttgtag ggtcggagcg
    37021 ttaacgttgc cagaggcgct ggagtacagc acgtaatgag cgccgacatt gcgggcaatg
    37081 cctatggctt tactacgggt gcctaaactg tcctgcggcg acaaacctaa ctgttgctta
    37141 gccatcgaca gttgctgggc ggaaaccagg gtaaatttcc cgttattagc cagcgcattt
    37201 cgcagcgttt cggtcgcttc tgcggcattc agcgaaccgt tagtacggtt gttaacgcta
    37261 tcaaccagca ggacgctacc cgcagtcacc ccgtcagccc caagcatctt actgaccatc
    37321 ggctgcattg cgccattcca gtcataatgg cgaatatgcg gcgcaggcgg tgccgttcga
    37381 tcttcgtgct caattgggcc tggctgctgc gggatcgtcg gcaccgaggg cactgtgggg
    37441 acaggctgtt gtggctcggc tggttgttcc ggcgctggtt tcacttcttc taccggtgca
    37501 ggttcacgtt gccccacaca cccggcgaga aacatcgcca gcgcggtaat caaggcgtag
    37561 cgactcattt ttgtcatcaa gattcacccc tttacaaata tagataaagt ctgactttgt
    37621 gcgcccccag aaaattggcg ctgccgtaca gcgttaccgc cgaatgtgcg ggaatggtaa
    37681 cgctgcgtgg cctttccaga ggatgcatct ccagccctct ggcgtcatac cagtaaaaac
    37741 gataatgaac ggtaacgggt tcttgccttt cgttataaag cgttgaggat gctgaaggtt
    37801 gaatatcaga cgtcgaaagg acgggctttt ctgcactgat gcccgcagcc aataaagatg
    37861 actccatcac cagcgattgc tcatcattca ccggaatttc cggatgtgaa cgacagccca
    37921 ccagcagtaa caacaccaga gacaccagcc caaagcatcc ttttctcatc gttacagacc
    37981 tttatgcgcc agcattggtc ccagcggacg gccacccaac aagtgcatat ggatgtggta
    38041 aacctcttgt ccgccatggc ggttggtatt catgatcaga cgatagccat cttcggcaat
    38101 accttcttgc tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg cctgctcatg
    38161 ctcagctgac acgtcgttca cagtcggaat gaggatattc ggaatgatca gaatatgcgt
    38221 tggcgcttgc ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt agacgatatc
    38281 ggaggggatc tcacgacgaa taattttgct gaatatagtt tcttctgcca cgacattttc
    38341 ctttttcaaa tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg agtatagagt
    38401 atggtcgagt taaacctgct ctttcaacct taactcacga tttcttaagc aaaaaatcgt
    38461 tttgctgccg tatatatcgc cattattccc atttctgcct gcgaatgcgc tgaaaagaca
    38521 ttctctgttt ggtttacacg acaaatatga atgcgtatat ttctcatttg catttgcaag
    38581 caaaattatt cgcacactaa atataaccgc tcatcaccct cctccggcat gagcctgacg
    38641 acaacataaa ccaagagatt tcaaatgctt tcaacacaat ttaacaggga taatcaatat
    38701 caagccatca ccaaaccgtc actacttgcc ggttgcatag cactggcact attaccttct
    38761 gccgcttttg ctgcaccagc cactgaagaa acggtgattg ttgagggttc agccacagct
    38821 ccagatgatg gcgaaaatga ttacagcgta acgtctacct ctgcgggtac caaaatgcag
    38881 atgactcaac gtgatattcc tcagtcggtc actattgtta gccagcagcg gatggaagat
    38941 cagcagttac aaacgctggg cgaagtgatg gaaaacacgc tggggatcag caaaagtcag
    39001 gcggattccg atcgtgctct ttattattcc cgcggattcc agatcgataa ctatatggtt
    39061 gatggtatcc ccacctattt tgaatcgcgc tggaatctgg gcgacgcact ttctgatatg
    39121 gcactgtttg aacgcgtaga agtagtgcgt ggcgcgacag gactcatgac cgggacgggt
    39181 aatccatctg cggcaattaa tatggttcga aaacacgcga ccagtcgtga atttaaaggc
    39241 gatgtctcgg cggaatacgg tagctggaac aaagaacggt atgtggcgga tttacaaagc
    39301 ccactcaccg aagacggtaa aatccgcgcg cgaattgtcg gcggctacca gaataacgac
    39361 tcatggctgg accgctacaa tagtgaaaag accttcttct cgggcattgt cgatgctgat
    39421 ttaggcgatc ttacgacgct ttcagccggt tacgaatatc agcgcattga tgttaatagc
    39481 ccaacctagg gcggtttacc gcgctggaat actgatggca gcagcaacag ttacgatcgc
    39541 gcacacagta ccgcacctga ctgggcgtac aacgataaag agatcaacaa ggtctttatg
    39601 accctgaagc agcggtttgc tgatacctgg caagcgacac tgaatgccac ccactctgaa
    39661 gtcgaatttg acagcaaaat gatgtatgtc gatgcctatg taaacaaagc ggatggtatg
    39721 ctggttgggc catacagtaa ttatggacct ggctttgatc atgtcggcgg caccggttgg
    39781 aacagtggca aacgtaaagt tgatgcgctg gatttgttcg ctgacggtag ttatgaattg
    39841 tttggtcgtc agcacaatct aatgtttggt ggcagttaca gcaaacaaaa caatcgttac
    39901 ttcagttcat gggccaacat cttcccggat gaaattggca gtttctacaa ctttaatggc
    39961 aatttcccac aaaccgactg gtcaccacag agcctggcgc aggacgatac cacacatatg
    40021 aaatcgttat atgctgccac tcgtgtcacc cttgccgatc cgctgcatct gatcctcggc
    40081 gcacgttata ccaactggcg ggttgatacg ctgacttaca gcatggagaa aaaccacacc
    40141 acgccttacg ctggtctggt gtttgacatc aatgacaact ggtcgaccta cgccagctat
    40201 acctctattt tccagccgca aaatgatcgt gacagttcag gcaaatatct ggctccaatc
    40261 accggtaaca actacgagct gggtctgaaa tcggactgga tgaatagccg tctgaccacc
    40321 acgttagcca tcttccgtat tgagcaggat aatgtcgctc agtccaccgg tacacctatc
    40381 cccggcagca acggcgaaac cgcctataaa gcggtggatg ggacagtcag taaaggggtg
    40441 gaatttgaac tcaacggcgc aattaccgac aactagcagc tgacatttgg cgcaacgcgc
    40501 tatcttgcag aggataacga aggaaacgcc gttaatccta atctgccacg caccacggtt
    40561 aaaatgttca ccagctatcg gttgcctgtc atgccagagt tgacagtcgg cggtggtgtt
    40621 aactggcaaa atcgcgtgta taccgacacc gtgacaccgt atggcacctt ccgcgccgag
    40681 caaggtagct acgcgctggt ggatctcttc acccgctacc aggtgacgaa aaacttctcg
    40741 ttacagggga acgtcaataa cctgttcgac aaaacctacg ataccaacat ggaaggttct
    40801 atcgtctacg gcgcaccgcg taatttcagc attaccggca cgtatcaatt ctgaataaca
    40861 cctgtaaaaa aggcagccat ctggctgcct tagtctcccc aacgtcttac ggattagtgg
    40921 ttacggatgt actcatccat ctcggttttc aggttatcgg atttagtacc gaaaatcgcc
    40981 tgaacaccag aaccagcaac cactacgccc gctgcgccca gtttcttcag gccagcctga
    41041 tccactttag acacatcagc aacgctgacg cgcagacggg taatacatgc gtcgaggtta
    41101 gtaatgtttt ctttaccacc aaatgcagca accagagccg gtgccatttc gctggtacct
    41161 gttgcttttg catcttcagt cgcgtcttca cgacccggcg ttttcagatc cagtgcttta
    41221 atcagcacgc ggaagatggt gtagtaaaca atcgcataac cgataccgac gatcgggaac
    41281 agccacagtt tgctgctgtt accagacaga acgatgaagt cgatcagacc gtgcgagaac
    41341 gacgtaccgt cacgcatccc cagaagaata cagattggga atgccaggcc tgccagaatc
    41401 gcgtggatga tgtacaggat cggcgcaacg aacatgaaga agaactcgat cggctcggtg
    41461 ataccggtca ggaacgaggt cagcgccgcg gagatcataa taccgcccac tttcgcgcgg
    41521 ttttctggtt tagcagagtg ccagatagca attgcggcag ctggcagacc gtacattttg
    41581 aacaggaagc caccagacag tttacccgca gtcgggtcac ccgccatata acgcggaatg
    41641 tcgccgtgga aaacctgacc tgctgcgttg gtgtattcac caatctgcat ctggaaaggt
    41701 acgttccaga tgtggtgcag accaaacggt accagacaac gttcgatgaa accgtaaatg
    41761 ccaaacgcaa ctaccgggtt ctggtaagca gcccactgag agaaggtctg gattgcagaa
    41821 ccaatcggcg gccaaatgaa ggacagcaca acgccagtaa agatggcagc caggccagaa
    41881 atgatcggca caaagcgttt accggcaaag aagccaagat actcaggcag cttaatacgg
    41941 tagaaacggt taaacatgta cgctgcaatc gcaccggaga taatccctcc gagtacgcca
    42001 gtatccgcca agtgtttaga ggcgatttct tcagcaggta aatgcagtac cagtggcgca
    42061 accacggcca tggttttaac catgatgcca taggcaacaa ctgcggccag cgcggatacg
    42121 ccatcgttat tggtaaagcc gagggcgaca ccgatcgcaa aaatcagtgg catgtttgca
    42181 aagacggaac cgcctgcttc tgccataaca tgcgatacaa cggcgggcag ccagctgaaa
    42241 ttcgcggaac cgacgcccag cagaatacct gcgataggta gtacggatac cggcagcatc
    42301 agcgatttac cgaccttttg caggttagca aatgcattct taaacataat tgagagtgct
    42361 cctgagtata ggtgcttttt ttacgttctc acgcgtggca aggggggaga gcctcgccgt
    42421 gtacagggca tctaagcgcc ctttatttat tacacagagt aaaataattc agtgccaatt
    42481 tgtttgacgg ctatcacgtt tcaacttcag atcattggag aagttgcaca ttttgacaaa
    42541 acatatgtca caatatttca aacgcggtta ttcaccgccc aattttcggc ggtgaacttt
    42601 actcgtttta gccattaatt acgagcttta aaaaaactca ttcaacggat ggattgaagg
    42661 cgggaagcgt cgatgtgaaa cagacgggcg aagttatcgg tggttacctg cgccagttct
    42721 tcaacggcaa cacctttcaa cacagccatg tattctgcaa cgtcacgaac catcgcaggt
    42781 tgattctctt ttcctcgatg cggtaccggc gcaaggtaag gtgagtcagt ttccaccagt
    42841 aatcgatcca gggggacata acgcgcagca tcgcacagtt gctccgcatt acggaaggtc
    42901 acaatgccgg aaaaggagat gtaaaatccg agatccagta atttacccgc cgtttctctg
    42961 tcctctgtaa aacagtgtag tacgccaccg caatccgtca ctttttcttc gcgcagaatt
    43021 gccagcgtat cggcacgggc gtcacgggta tggacgataa ccggcttgtt cagttcacga
    43081 ccaatctgga tatgatggat gaaggactct tgctgacgta ctttagtttc cggtgtgtaa
    43141 taataatcca gcccggtttc acccagcgct acaacaccct cttctgccgc cagacggcgt
    43201 aaatcttcta catcgtaggg atcattctgg ttgagtggat gcacgccaca agaaaatacg
    43261 acgttgtcac gttcgcctac cagatcccgc atatgtaaat aacccggtaa tgttgtggcg
    43321 actgccagac aaaatttcac atcgcgtgcg gcggctttcg ccagaacgtc atccacgtcc
    43381 ttatgcaaag attcataatc cagaccatcg agatggcagt gtgagtcgac taaaaacatg
    43441 atgtctctct taaagatgag gaaccggtag cacaacgccc ggttgcaggt aatgctcaat
    43501 acgcagtaaa agatcggtga tgagaagctc gcggttgatg cctgtgacag acattaactg
    43561 ttcacgaatg tggcaaacat cccccagtat agcctgcagg cgcgagggag aaagatggtt
    43621 tgccagttcg gcgaccaggc ccggcacatc aacattggtc acctgcgcag cgccatgatg
    43681 gcgttttagc gcatccatca gcaacgttgc cacccagtgt aaacgcgccg gagcttgttc
    43741 atgattaagg gccgccagca gcgaatacca gtcgcccgat ggcacgctat atgccaacgc
    43801 ctgacacaat gtttcacgag cctgccagtt atctccctga aacaacgcca gtgccgcgcc
    43861 aggcgaaccg gcgcttaagc gcaatgcggc aagtaatgca tcctgtgaca ttgtcacttc
    43921 gcgtgaaagc caagtcacgg cgtactgttc tggaggcggc gcaaggtaat gtaaccgaca
    43981 acgactacgt aatgttgcca gtaaacgttc aggctcgcgg gtagccagga aaaaccaggt
    44041 ttctgctggt ggctcttcaa gcgttttcag caatgcgtta gccgcggcgt cggtcagtaa
    44101 ggcagcatcg gttacccaga caactttcgc accacctagg cgtgcgtgct cattcagctt
    44161 ttcggtgacc tcacgtaccg catcaatgcc cagcgtattt ttcccttttt cgggagccag
    44221 ggtgtagtaa tcgggatgcg tgccagcctg catcaactga catccacgac agtgaccgca
    44281 acttttgtgg ccctgcggtt gttggcagag taaataacgg ctcagggcgt agattaaagc
    44341 atcatcgccc atgcccggta acgcctgaat gagtagcgca tggtgacctc ttccggcctg
    44401 atagctggct accagttttt cgaaatcagg tcgtaaccat ggataccatc tcatgcgtcc
    44461 aactccttca cccagtgggt cacggtagtg cggattgcat ccatcacggc ctccagcggc
    44521 tgggtagcat caatggtatg aatactttta tcttgtgcag ccagttccag atagcgggcg
    44581 cgggtgcgat taaagaaatc gaaagactct tgctcaatac gatccagctc gccgcgcgta
    44641 cgcgcgcgtt ttaagccaac ttccggggta acatcgagat agagcgttaa gtcggggcga
    44701 aaatccccga gaacagcatc acgcagtgtt gccagcatat gttggtcaat accacgtcct
    44761 ccgccctgat acgcctgagt ggagagatcg tggcgatcgc caatcaccca ggtgccgtta
    44821 gccagcgcag gtttgatgac cgtttctacc agttgaacgc gcgcggcata aaacatcaga
    44881 acttcggctt tatcggtaat gacttcatcg cctaccgatt tgatatccag caccaggctt
    44941 cttaactttt cggcaagttg cgtaccgcca ggttcccgag tgaaaaccat gtcgcggata
    45001 cccagttgct cgagcgtctc aaccaccaca ttacgcgcgg tagttttgcc tgcgccttcc
    45061 agcccctcaa tgacgatata cttactgcgc atttttttcc ttaagcactt tcagataatc
    45121 ctgcacagac ttgttatgac tggcaagatt ggtattaaac gtgtgaccac ctttaccatc
    45181 ggccacaaaa tagagatacg gcgtttttgc cggatgcgca gcagccttca gcgaatccgc
    45241 ccccggcgta gctatcgcac ccggcggcaa accggtaatg gtataggtgt tatacgctgt
    45301 cggcgtttcc aggtctgcac gagaaagttt gccattataa cgctctccca tcccgtaaat
    45361 cacggttggg tcggtctgca ggcgcatacc aatgcgtaaa cggttgataa ataccgaggc
    45421 aacctgatcg cgttcactgg caacggcggt ttctttttcg ataattgatg ccatcgtcac
    45481 cagctgattt ttatctttat aaggcagacc gtccgcacgc ccttcccagg cgctatcgac
    45541 cgctttcacc attttcttgt gagctcgctt gagtaacgcg acatcggtgg tattggcggt
    45601 atacatcaag gtgtctggcc agaaccaacc ttcaatccac tccgggtttt ccagttcaag
    45661 tgcctgcgct acggtggcgt acttatcgtc actcagcgtg tgcttgatat acggagcctc
    45721 acgcaattgc ttgaggtagt cgctcagacg catcccttct accagtcgca gcgggaactg
    45781 tgcttcttta ccgctttcca acaatttcag catctcgcgc acggtcatct gcggtgtaaa
    45841 gcggtaagtc ccggctttaa agtgagaaag atccggttcg atacgcagca gccattgaaa
    45901 aacccgtggg cgattgatga tcttatcagc ataaagctgt tcaccgagcg ccagacgtcc
    45961 ggtccctggc ttcagggtaa atatcgtctc ttctttgata agcaatttgc tgtcggcaag
    46021 atggcgaacc ttccagacgc ccacaccagc ggcgataccc agtaccacca gcaataacaa
    46081 gataatcaat aacacttttt tcatgactaa ttcgggcgct cacaaagtgg ggctaaatat
    46141 tcatataacg ttgctgacga aaaggtgaca tcgccacagg cacgtacggg catcactggc
    46201 attaacgcat tacaaataac catctcatct gcctgcaacg cctcctccag cgaggcttgc
    46261 acttcgacaa gctgataaga ggattgtgcc agtaaacgga tacagaattg tcgcataatg
    46321 ccgttaacac ctgcctgatc cagtcgcggc gtataaacta cattgccctt ccgccagaac
    46381 aaattagccg cacagcattc cgtaacccac ccttcgctgt caaggaccag cgcctcatca
    46441 gcgtttgtct gctcaagatg agagcgaatc aatacttgct caagccgatt aagatgttta
    46501 atacctgcaa gatgaggatt gcgccccagc cgcaccgggc ttagcgccaa cgtcatccct
    46561 tcgttacgca aacggtcgta atgtgcagga taagccgtaa cggagagaat ccgcgttgct
    46621 ggtccgctgt tcaatgtgct gtaccctcgc ccgccactac cgcgactgat cacgactttc
    46681 agcacaccat tttgctgttc tgctgccagc gttttcatct cttgttcaag ctgaggccag
    46741 aagtcacagg aaatcatcaa ccgctgacaa gcatcctgta gtcgctggat atgcgccgat
    46801 aacaaactga ctttaccgtc gataactctg gcggtggtaa aacaaccatc accaaactgc
    46861 gttgcccgat cgcttactgc cagcgattcc tgcttataac cgttaattaa gaacataccg
    46921 gctccttatc agagaattgt tagtgtggca gcatgttcac tacggaacaa gtcggaataa
    46981 aagctaagaa aaaaggcccg caagcggacc ttttatatgg gtggaaaatg acaacttaga
    47041 tctttttaaa gatcaaagaa ccattagtgc caccgaagcc gaaggagtta cacagcgtgt
    47101 attccattcc gctgacctga cgtgcttcat gcggtacgaa atccagatcg caaccttcat
    47161 ccgggttatc caggttgatg gttggcggaa cagcctgatc gcgcagcgcc aggatggagt
    47221 agatagattc tactgcaccc gccgcaccta acaggtgacc ggtcatagat ttcgtggagc
    47281 ttaccaacac acggcttgca gcttcaccga agatggtttt caccgcctgc gcttcagctt
    47341 tatcgccagc cggcgtagaa gtaccgtgcg cgttaacgta gccaatctga ctcgcttcaa
    47401 tgcctgcatc acgcagagca tttgccattg ccagagctgc gcctgcgcca ttttctggcg
    47461 gtgacgtcat atgataagca tcgctgctca taccaaagcc gacgagttca gcgtaaattt
    47521 tcgcaccgcg ttttttcgcg tgttcgtact cttcaagtac cagcataccg gcaccatcgc
    47581 ccagtacgaa gccatcacgc tctttatccc acgggcggct cgccgcttgc gggttatcat
    47641 tgcgggtaga taatgcacgt gccgcgccaa aaccaccaac gcccagcggc gtactggctt
    47701 tctctgcgcc acctgcaacc atcacgtcag catcgccata cgcgataata cgcgcagcat
    47761 ggccaatgtt gtgcacgccg gaagtacagg cggtcgcgat agagatgctt gggccacgca
    47821 ggccatacat gatagtcaga tgacctgcca ccatgttcac aatcgttgac ggaacgaaga
    47881 atgggctgat cttacgtgga ccaccgttca tcagagatgt gtggttttct tcgatcagtc
    47941 cgaggccgcc aatcccggag ccaattgcgg caccaatgcg ggttgcgttc tcttccgtta
    48001 tttcaaggcc agaatcctgc atggcctgaa cgccagcgac aattccatat tgaatgaagg
    48061 catccatctt gcgctgttct ttgcgcgaga taatgtcctc acagttaaaa tcctttacta
    48121 agccagcaaa tttcgttgca taggcgctag tatcgaaatg gtcgattagg ctgatgccac
    48181 tctgaccggc aagcagagct ttccaggtag actctacggt attgccgaca ggagacaaca
    48241 tgcccagtcc ggtcacaact acacgacgct tagacacgtt tgtcctccaa ggagggaaaa
    48301 atgattctag tgggacaaaa agataaaact caggcggtcg aacgaccgcc tggagatgtt
    48361 cacttacgcc tggtggccgt tgatgtaatc aatggcagcc tgaacggtgg tgattttctc
    48421 agcttcttcg tccggaatct cagtatcaaa ctcttcttcc agagccatta ccagctcaac
    48481 ggtgtcaaga gaatccgcgc ccaggtcttc aacgaaagaa gcattgttgg taacttcttc
    48541 ctgcttaacg cccagctgtt cgccgataat tttcttaacg cgttcttcga tagtgctcat
    48601 actcttaaat ttcctatcaa aactcgcttt cgcgatggtt ttcgtagtgt ataaaatgtt
    48661 gaaaaatttg caactaaatc ccggcaggtc ttaccacgat tttacgttat tttgaggcct
    48721 taccccccaa taacgcaaat cattttgcac taattattac tcataaccac gcagactgcg
    48781 cgcaacatga gcattttgtg caaatcgcgg tcagaccatg tacatcccgc cgttcacatg
    48841 caaagtttca cccgtgatgt aagctgcttc gtcggatgcc aggaatgcaa ccgcgttggc
    48901 gatttcctgt gcgccgccga ggcgacccgc aggaacctgc gccaggatac ccgcacgctg
    48961 gtcatcgctc agcgcacgtg tcatgtccgt ttcaataaag cccggagcaa caacgtttac
    49021 agtaataccg cgtgacgcaa cttcgcgcgc cagtgattta ctgaagccga tcaagcccgc
    49081 tttcgccgca gcgtagttgg cctgaccgcc atttcccatg gtaccaacca cagaaccgat
    49141 agtgataata cgaccatgac gctttttcat catagcgcgc attaccgctt ttgacagacg
    49201 gaaaacagat gaaaggttag tttcgataat atcgttccac tcttcatctt tcattcgcat
    49261 taacaggtta tcacgagtga taccggcatt attgaccagg atatccactt caccaaattc
    49321 tgcgcgaatt ttttccagaa cagattcgat agatgccggg tcggtcacat tcaacatcag
    49381 acctttgccg ttggcaccta aataatcact gatcgcctga gcgccatttt cactggtcgc
    49441 agtgccaata actttcgcgc cacgggctgc gagcgtttca gcaattgcgc ggccaattcc
    49501 gcggcttgca ccggttacca gtgcgatttt tccttcaaaa ttcatgattt tcctctttta
    49561 aagctcgagc gccgctgcca tcgctgaagg ttcgttcagc gccgaggcgg tcagggtgtc
    49621 gacaatgcgt ttcgtcaggc cagtaagcac cttgcccggg ccgacttcat agagatgttc
    49681 tacgccttgc gctgccatgt actcaacaga cttcgtccac tgaaccgggt tatacaactg
    49741 acgtaccagt gcgtcacgga tggcatcacc attggtttcg catttcacat caacgttatt
    49801 cacaacagaa actgttggtg cgttaaaggt gattttcgct aattctactg ccagtttgtc
    49861 ggctgctggt ttcatcagcg cacagtgaga cggtacgctc actggtaacg gcagcgcgcg
    49921 ttttgcgcca gcagctttac aggcagcgcc agcacgctca accgcttctt tatgaccggc
    49981 aataaccacc tgtcccggag agttaaagtt taccggagaa acgacctgac cttctgcagc
    50041 ttcttcacac gctttcgcaa tagacgcatc atccagaccg atgattgccg ccatagcgcc
    50101 cgtgccttcc ggtacggctt cttgcatgaa cttaccgcgc atctcaacca aacgcaccgc
    50161 atcagcgaaa tcaatcacgc cagcgcaaac cagcgcggag tattccccca ggctgtgacc
    50221 ggccatcatt gccggtgctt taccgccctg ctgctgccat acgcgataca gcgcaacaga
    50281 tgcagtcaac agcgctggct gggtttgcca ggttttattc agttcttcag ctggcccctg
    50341 ctgggtcagc gcccacaggt cgtagcccag cgccgcagaa gcttcagcaa acgtttcttc
    50401 gacaattgga tagctcgccg ccatatcagc cagcattcca acggtttgag aaccctgtcc
    50461 agggaacaca aatgcaaatt gcgtcatgtt ttaatcctta tcctagaaac gaaccagcgc
    50521 ggagccccaa gtgaatccac cgccaaaggc ttcaagcaga accaactgcc ccggcttaat
    50581 gcgcccgtcg cgtacagctt catccagcgc gcacgggaca gaggccgcag aggtattacc
    50641 gtggcgatcc agcgtcacca cgacattgtc catcgacata ccgagttttt tcgccgttgc
    50701 actgataata cgcaggttag cctgatgcgg aaccagccag tccagttgag aacggtcaag
    50761 attattcgcc gccagcgtct catcaacgat gtgcgccagt tccgttaccg caaccttgaa
    50821 gacttcgttg cccgccatcg tcagatgaat tgaattctct ggattcacgc ggtcggcgtt
    50881 tggcagcgtc agcaattcac cataactacc gtcggcatgc agatgggtgg aaatgattcc
    50941 cggctcttca gaggcagcca gcaccgcagc gcccgcgcca tcgccaaaaa taataatagt
    51001 cccacgatct gttggatcgc aggtgcgcgc cagtacatcg gaaccgacga ccagagcata
    51061 cttcaccgcc ccagatttca cgtattgatc ggctacgctt aatgcatagg tgaaacctgc
    51121 gcaggctgct gcaacgtcaa atgccgggca acctttaatg cccaacatgc tttgaatctg
    51181 acaagctgcg ctcgggaaag cgtgcgtagc agaagtcgtt gccacaacga tcaggccaat
    51241 ctggtctttc tcaatgcccg ccatctcaat tgcgcgtgtc gccgcttcaa agcccatggt
    51301 tgaaacggtt tcgtttggcg cggcaatgtg gcgttcgcgg ataccggtac gagtgacaat
    51361 ccactcgtca gaggtgtcca ccattttttc caaatcggcg tttgtccgca cttgttcggg
    51421 cagatagctg ccagtaccaa taatcttcgt atacatgtac gctcagtcac ttttcggtta
    51481 tataccgtca cttgcaaact gcgagttcgc tggcagcgtc ctgctaccgc agagttccgc
    51541 ttttgccacc gtccagcagc tcaaaaccag ctgggtatac agattccagg cgagcggcaa
    51601 ttcgctgagg aacttgtcgc tgcaccgcct gcactgcctg ttcaatcgcg accgcaaaag
    51661 ctcgctgatt ggctgcacca tgacttttta tcaccgtgcc gcgcaatcct aacagacagg
    51721 cgccgttata ctggtcgggg ttgaggtgac tgaatcgcct cgtcaggctc ttttgtagcc
    51781 aacgctttaa taacagtagc caccacgacc gttttttccc ttcaccctga gatttcagca
    51841 gagaaaggaa catcctgaca acaccttcca tcgtctttaa cgtgacattt cctgtaaagc
    51901 cgtcacaaac cagcacatct gtcttgccag ttaacaactc attggcttca agatagccga
    51961 tataattgat agaagggatt gttttaagca ccgctgaggc atcccgaata ctgtcaagac
    52021 cctttacttc ttcttcacca atattgagca acgccacgcg aggattggga atttccacca
    52081 cctcttcagc cagaactgag cccataatgg caaattgcac caacattgtg ctgtcacaat
    52141 cgacgttggc ccctaaatcg aggaccaccg ttttgccctt ttgctgatgt ggtaataccg
    52201 tcaccagcgc cggacgctca atcccctcca ggggcttgag taataatttt gccagcccca
    52261 tcagtgcccc ggtattaccg gcactgacac aggcttgcgc tcgaccttct ttcaccagct
    52321 ccagggccat acgcattgaa ctcccacgac tggcgcggat agcttgcgaa ggccgggcat
    52381 cactggcgat aactgactgc gcaggaataa tctgcagacg cgaacgttgt tcaaagtcag
    52441 ctttagcaag taatggcgtg atggcgtcgg gattgccgac taaaagaaga gtgagttgcg
    52501 aattagaatt cagtgcctgc aatgctgcag gcactgtcac ggaagggcca aaatcccctc
    52561 ccatgacatc taacgccagg gttagacgtg tcaaggtatc gtcgccgccc ggtttggtct
    52621 ttccccagtt gcctggggaa aatcctcact aagcttcatc acgcagacgc gtgattactt
    52681 agcgatgacc ttgcggccgc ggtagtaacc gtcggcagtg atgtggtgac gcaggtgttt
    52741 ttcaccagaa gttttgtcta cagacaggct ggtgactgcg gtcagcgcgt catgggaacg
    52801 acgcatgcca cgtttggaac gggttggttt attctgttgt acggccatgg accttactcc
    52861 tcaattacgg tttatccccg atccaacggg gagcaccaat tacttacgct ttaagctggc
    52921 taatacggca aatgggtttg gcttttgcac ttcttcaggc agttcaccaa agaccatgtc
    52981 cgcttcggac acttcacagt gttcagaatc atgcaccgga actaccggca aggcgaggat
    53041 gatttcatct tcaaccattg caagcagatc gatttcaccg aattcgttaa cctcaatcgg
    53101 ttcatacgct tccggcagtg cttcagcctg ttcgtctgaa cgcacaggac taaaacaata
    53161 cgttgtgtag acctgatgag taaacggctt cccgcaacgc tgacactcga gcgttaccgt
    53221 caccttcgca tcgccgttta acactgcgag acgttggtta tcgatagcga acgacatgga
    53281 gcattccaca tcactgtcca cactgactac ggattcggcg acgcgctcaa cctgatcagg
    53341 ggtatagata ccctggtaat caaggcgttt ttgagccgta cgaaccggat cgagagtcag
    53401 ggggaatttt accttttgca tagggcgcgc atattaactt tgtaacgtca tagagtcaaa
    53461 gaaaaaggca gcctgtggtt gccttttgcc aataattcgc acacattgcg ggttactgct
    53521 ttattttcgt tcagtgctga taacgaacat caacctgcaa aacgaagcgc atagtttaaa
    53581 atggctatca tcaatacgct atatctgatg gaaaaaaata tgcctaaact tattttagcc
    53641 tccacctcgc catggcgtcg cgctcttctg gaaaaactgc aaatctcttt tgaatgtgca
    53701 gcacctgagg tcgacgaaac cccacgcagc gacgaatcac cgcgacagtt ggtgcttcga
    53761 ctggcacaag aaaaagcgca atctctggcg tcacgttatc cggatcattt aattattggt
    53821 tcagaccagg tatgtgttct tgatggcgaa atcaccggta aaccgttaac ggaagaaaat
    53881 gcccgtctgc aattacgcaa agccagcggc aatatcgtca ccttctatac tggactggcg
    53941 ttgtttaatt cggcgaatgg gcatctacaa acagaagtgg agccttttga cgtccatttc
    54001 cgtcacctaa gcgaggcgga gattgataat tacgtgcgta aggagcatcc cctgcactgc
    54061 gcgggtagct ttaagagtga aggatttggc attacgctgt ttgagcgttt agagggacgt
    54121 gaccctaaca cgctggttgg tttaccgcta atcgcgctgt gtcagatgtt acgtcgggaa
    54181 gggaaaaacc cgctgatggg atagttgttt gcgccggatg cggttacgtc gcatccggca
    54241 atcggtgcag gcggcctgat gcggcgtaaa cgccttatca ggccaaccgc ccattatcag
    54301 gcttatatgc ttgtattagc gcgcgttacg cagtttttgc aaacaacgct tcaaaccttc
    54361 atccatcggc gcttcgatac gcatcacctc accggtcccc ggatgggtaa acttcaacgc
    54421 tgcggcgtgc aggaacagac gatttaatcc cgtgcctgct tcagtgagct gtctgtcaaa
    54481 ttcacggtca ccgtagcgat cgtcaaaggc aatcggatga cccgcatact gtgtatgcac
    54541 acggatctga tgagtacgtc cggttaccgg gctacaacgc accagggtgg caaatgcata
    54601 gcgttcttcc actttaaagc gtgtttccga cggtttgcct tcctgactga cacgcacgat
    54661 acgttcgccg ctttgcagaa tatttttcaa taacggcgct tgaacgctct tcacatgcga
    54721 ctgccactga ccgcgcacca gcgccaggta atctttttgc attccttttt cacgtaattg
    54781 ctcatgcaga gaacgcaacg ccgagcgttt tttcgctacc agcaacacac ctgaagtgtc
    54841 ccggtcgaga cgatgaacca gttcaaggaa ccgcgcttcc gggcgcaacg cccgcaaacc
    54901 ttcgataacg ccgaagctta aaccactgcc gccatgtacc gccgtaccgg aaggtttatt
    54961 cagcaccagg atatgatcat cttcatataa gatgacgtcc gccagcgccg ccaccttttg
    55021 cagatgtggc gaaaccgcct cttcttcccg ctcagcaacg cgaaccggtg ggatacgcac
    55081 ctcatcaccc gcttcgagtt tatattcagg cttaatacgt tttttgttca cccgcacttc
    55141 acctttacgc aaaatacggt aaatcatact ttttggtacg cctttcaatt gggtacgcaa
    55201 aaagttatcg atacgttgcc ccgcttcgtc agcggtgata gcaacaattt ttacggatgg
    55261 agtctctgtt ttcatggtgg gcgattctaa ttagccaaca ggattcgcgc cactcatttt
    55321 tctatgctta tatttagttt tgcaccttat tacttcacta cgtgatcact ttattgatgg
    55381 ttattaaacc aatcaccagc aagaagtgaa aaaactgtga gtaagcgggt gataaatggt
    55441 aaaagtcatc ttgctataac aaggcttgca gtggaataat gaggccgttt ccgtgtccat
    55501 ccttgttaaa acaagaaatt ttacggaata acccattttg cccgaccgat catccacgca
    55561 gcaatggcgt aagacgtatt gatctttcag gcagttagcg ggctgcgggt tgcagtcctt
    55621 accggtagat ggaaatattt ctggagagta atacccagtc tgtttctttg ataattgcgc
    55681 tgtttttccg catgaaaaac gggcaaccga cactctgcgc ctctttgagc tgacgataac
    55741 cgtgaggttg gcgacgcgac tagacacgag gccatcggtt cacacccgga aaggcgttac
    55801 tttgcccgca gcttagtcgt caatgtaaga ataatgagta agttacgatg aaaagaatgt
    55861 taatcaacgc aactcagcag gaagagttgc gcgttgccct tgtagatggg cagcgtctgt
    55921 atgacctgga tatcgaaagt ccagggcacg agcagaaaaa ggcaaacatc tacaaaggta
    55981 aaatcacccg cattgaaccg agtctggaag ctgcttttgt tgattacggc gctgaacgtc
    56041 acggtttcct cccactaaaa gaaattgccc gcgaatattt ccctgctaac tacagtgctc
    56101 atggtcgtcc caacattaaa gatgtgttgc gtgaaggtca ggaagtcatt gttcagatcg
    56161 ataaagaaga gcgcggcaac aaaggcgcgg cattaaccac ctttatcagt ctggcgggta
    56221 gctacctggt tctgatgcca aataacccgc gcgcaggtgg catttctcgc cgtatcgaag
    56281 gcgatgaccg taccgaatta aaagaagcac tggcaagcct tgaactgccg gaaggcatgg
    56341 ggcttatcgt gcgcaccgct ggcgtaggca aatctgctga ggcgctgcaa tgggatttaa
    56401 gcttccgcct gaaacactgg gaagccatca aaaaagccgc tgaaagccgc ccggctccgt
    56461 tcctgattca tcaggagagc aacgtaatcg ttcgcgcctt ccgcgattat ttacgtcagg
    56521 atatcggcga aatccttatc gataacccga aagtcctcga actggcacgt cagcatatcg
    56581 ctgcattagg tcgcccggat ttcagcagca aaatcaaact gtacaccggc gagatcccgc
    56641 tgttcagcca ctaccagatc gagtcgcaga tcgagtccgc cttccagcgt gaagtccgtc
    56701 tgccatccgg tggttccatt gttatcgaca gcaccgaagc gttaacagcc atcgacatca
    56761 actccgcacg cgcgacccgc ggcggcgata tcgaagaaac tgcgtttaac accaacctcg
    56821 aagccgccga tgagattgct cgtcagctgc gcctgcgtga cctcggcggc ctgattgtta
    56881 tcgacttcat cgacatgacg ccagtacgcc accagcgtgc ggtagaaaac cgtctgcgtg
    56941 aagcggtgcg tcaggaccgt gcgcgtattc aaatcagcca tatttctcgc tttggcctgc
    57001 tggaaatgtc ccgccagcgc ctgagcccat cactgggtga atccagccat cacgtctgcc
    57061 cgcgctgctc cggtaccggt accgtgcgtg acaacgaatc gctgtcgctc tctattctgc
    57121 gtttgatcga agaagaagcg ctgaaagaga acacccagga agttcacgcc attgttcctg
    57181 tgccaatcgc ttcttatctg ctgaatgaaa aacgttctgc ggtgaatgcg attgaaacgc
    57241 gtcaggacgg tgttcgctgc gtgattgtgc caaacgatca gatggaaacc ccgcactacc
    57301 acgtgttgcg cgtgcgcaaa ggggaagaaa cgccgacctt aagctacatg ctgccgaagc
    57361 tgcatgaaga agcgatggcg ctgccgtctg aagaagagtt cgctgaacgt aagcgtcctg
    57421 aacaacctgc actggcaacc tttgccatgc cggatgtgcc gccagcgcca accccagctg
    57481 aacctgccgc gactgtcgta gccccagcac cgaaagctgc aacggcaaca ccagcagctc
    57541 ctgcacaacc tgggctgtta agccgcttct tcggcgcact gaaagcgctg ttcagcggtg
    57601 gtgaagaagc caaaccgacc gagcaaccaa caccgaaagc agaagcgaaa ccggaacgtc
    57661 aacaggatcg tcgcaaacct cgtcagagca accgccgtga ccgtaatgag cgccgcgaca
    57721 cccgtagtga acgtactgaa ggcaacgata atcgcgaaga aaaccgtcgt aatcgtcgcc
    57781 aggcacagca gcaaactgcc gagacgcgtg agagccgtca gcaggctgag gtaacggaaa
    57841 aagcacgtac caccgacgag cagcaagcgc cgcgtcgtga acgtagccgc cgccgtaacg
    57901 atgataaacg tcaggcgcaa caagaagcga aggcgctgaa tgttgaagag caatctgttc
    57961 aggaaaccga acaggaagaa cgtgtacgtc cggttcagcc gcgtcgtaaa cagcgtcagc
    58021 tcaatcagaa agtgcgttac gagcaaagcg tagccgaaga agcggtagtc gcaccggtgg
    58081 ttgaagaaac tgccgctgcc gaaccaattg ttcaggaagc tccagctcca cgcacagaac
    58141 tggtgaaagt cccgctgcca gtcgtagcgc aaactgcacc agaacagcaa gaagagaaca
    58201 atgctgataa ccgtgacaac ggtggcatgc cgcgtcgttc tcgccgctcg cctcgtcacc
    58261 tgcgcgtgag tggtcagcgt cgtcgtcgct atcgtgacga gcgttatcca acccagtcgc
    58321 caatgccgtt gaccgtagcg tgcgcgtctc cggaactggc ctctggcaaa gtctggatcc
    58381 gctatccaat tgtacgtccg caagatgtac aagttgaaga gcagcgcgaa caggaagaag
    58441 tacaagtgca gccgatggtg actgaggtcc ctgtcgccgc cgctgtcgaa cctgttgtta
    58501 gcgcgccagt tgttgaagaa atggctgaag tcgtagaagc ccccgtaccg gttgccgaac
    58561 cgcaaccgga agtggttgaa acgacgcatc ctgaagtgat tgccgccgcg gtaactgaac
    58621 agccgcaggt gattaccgag tctgatgttg ccgtagccca ggaagtcgca gaacacgcag
    58681 aaccggtggt tgaaccgcag gaagagacgg cagatattga agaagttgcc gaaactgctg
    58741 aggttgtggt tgctgaacct gaagttgttg ctcaacctgc cgcgccagtc gtcgctgaag
    58801 tcgcagcaga agttgaaacg gtagccgcgg ttgaaccaga gatcaccgtt gagcataacc
    58861 acgctaccgc gccaatgacg cgcgctccgg caccggaata tgttccggag gcaccgcgtc
    58921 acagtgactg gcagcgccct acttttgcct tcgaaggtaa aggtgccgca ggtggtcata
    58981 cggcaacgca tcatgcctct gccgctcctg cgcgtccgca acctgttgag taataattag
    59041 ctcaaagtaa tcaagccctg gtaactgcca gggctttttt atttcatgtt tgaatcaaca
    59101 tattaaaatt catcaaatgg catccttgct aaccaacaat gcaaaatagg caaaaaaaat
    59161 gcccgccccg gcgatcccag agcgggcaga caaaacatac ccagtttcat gatatgtttt
    59221 aaagcgagct tatttgttga gctggaagag cgacaatccc tgcatatcgg taaatgcttt
    59281 atacgatgcc tgcaatgccg tttgctgcat gatgtaagat gaaatagttg cattccagtc
    59341 cacatcaacc agatcgctca tctgctgcgt ttgccctaaa gcgcgatcgc tacctaagga
    59401 atccagcgac tccagtttgt tcagctgcgt gcctaattcc gtgcgaacgg taaggacatt
    59461 attcagcgag tttttcagtc cacggttggt tttatccaat gccgcagcgg cgatttcttt
    59521 atccgcttcg ctatccgcga ccggcgtttt cagggctgcg atggcgctat ccagcatggc
    59581 aaaaagattg gtttcagaag cgctaccgtc tggttccgct accgcgttac tggtaatact
    59641 gtcgaaaatt ttgtcgcccg tatgtcctat caccatcgaa cgcgaagcat cgacctgttg
    59701 cttaatactt tctgttccgc cgacataatc accgttagcc tcactaaacg gcgcagtctc
    59761 tgttttataa ccggcaaaaa tgtagcgccc attaccgtca gtggtgttcg ccagattcag
    59821 caactggtca cgaagcccct gaatatccgt agccagcgag gcccggtcat cgtcactcaa
    59881 ggtgccattg ctggcgtaga caattttttc ctgagcattc tggatagcag tggtgacctg
    59941 gctaagtaca ctctcttcca gtgacacttt ttgagtggcg aaagtacgtg ccagcgtgta
    60001 ctggctgttt tgcgcctgtg cctgggagag aacaacagct tgtgatgcag caatgggatc
    60061 gtctgaaggg ttaacgactc gcttacccgt caacatctgc tcgccgtact tcatccattc
    60121 tgcctgagaa ttggtgatgc cgcgcatgtt ttgctggtac atcatctggg tactgaaacg
    60181 cattttatct ccccttagcg aatgttaatc agcgcatcaa aaatcgcgtt tgccgtctgc
    60241 agaacctgcg cattcgccag gtaatactgc tgaaaacgtt gcagatttcc gtactcctca
    60301 tcgagattga caccggaaat cgactgctgc tgattggaaa gctgcgtcac cacattacct
    60361 tgcgtggtgc tactggtttt caacgtcgcg gttttattac cgatatcact cactaacgag
    60421 gcataagcgt cgttaaagga tttcgcaccg cccaccgttt tactgttgct ttgcagatcc
    60481 agcagggcct gaccgttgcg gttgtcgcta tcacccgcat cttcttcgct cgccatcgcg
    60541 attttcgctt cgtcggtgat taatacatcc atgttgacga tggcgtcact tactggtttc
    60601 agcgtgaagc tgtcgttaac ggcaggcgtt cctgtaaacg tcaactccag accatcaaat
    60661 gccactttac cgttggcatc tggtgtcgcc gtaaaagtgg tattgctggc aaggcgggtg
    60721 acctgccact gattattatc gaacgagatt ttgtaatctg tcgccagtac cacggaggca
    60781 tcagttaccg tggcaccgat cgcaacgtca cctttgtttt tcgtgttttg ctgaaccgcg
    60841 ggcttaccga tagtaaagaa atcttcacca gcatcgccgt tagcatcaaa tccggctttg
    60901 tgttggctgt tgaaagcctc ggcaaatgcc agcgccagtt gtccaagcgt attacgcgtc
    60961 tggtccagat cctgagaacg gaatgtcaga atgccgccca gcgacccggt attcagtaat
    61021 ttctccggga tctcaatatt gcctgccgtc ccatcaacgt aagcgacagt cgtacgagaa
    61081 gggtcggcgc tggaaggaac tgccgccagt tgccgcgccg tacttccctg aaccagtgag
    61141 taaccattgg catcgtgatg ttataagtac cgccatcctg aacgctgact tctacgccaa
    61201 caatctggtt taattcgctc accagttgat cgcgttgatc cagcagattg ttaggtgacg
    61261 cccctgcccc cacgcctgtc aggcgcgaga tttgatcgtt caggctggca atttgtttag
    61321 cgtaattgtt gatctgatca acgctggcac ctatcgcgat attgacctgt ttgtcctggt
    61381 cgcgcagata ttgatcggtg gttttaaact gattcaccaa cccttctgat ttcccaatca
    61441 gcgcctggcg cgccgccggg tcttccgcgt tactcaccag cgtttgcagg ctggtgaaga
    61501 aatcctgcat ctgtgttgcc agcgaagagg tactggtgga gagcatattg tcgattttcg
    61561 acatctgctc atagcgggcg gtcaggccgc tactttgcgt ctgcacagca cgtaactggt
    61621 tggtaataaa cgtatcatac tcacgctgca caccagaaac gtagacgcca ttgccaaccc
    61681 agccgccagc gcccaacgtg ctattggcct gcgccataat agtggtttgg cgggtatatc
    61741 cggcaacgtt atagctggag atattattac ttgccgtatt taacgccgcc tgggccgcgt
    61801 tcagtccgct catggcgtta ttgatcaagc tggacatgga ggttccttat aagccttcaa
    61861 ttacagagta ttatcggcag cgacccgccg gacttgagtt attcagaaca gattatcaat
    61921 gttcatgctg taggttttgc tcacctggtc gcttatcgat ttcatctgct ggatcatgtt
    61981 ggtgagttta cgggcatagt gaggatcggt agcatagccc gcgtcctgta ggacctgcgc
    62041 cccctgttcc gcactcgcgg cggtcgtcac ggcggcgtag cgcgggttac gtgttaacag
    62101 cccaacgtaa tccgacaatg cttccagata cgagctgtag acccgaaact tcgcttttac
    62161 tttcttcgct tcgccgtttt catattcagt cgtggtgatt tcagtgactt gccctttcca
    62221 gttgccagag gctttgacac caaacaggtt atagcttggc tcgccgtttt cgcggcggat
    62281 ttgccgttgc ccccaaccag attcaagcgc agcctgagcg aggatcaaat gatgtggcac
    62341 accgctttgc tggcttgcca gttgggcggg cagcgagagt tgcgcgagga atgctctact
    62401 gtcacccggc agcgaatcat cgtagttacg tggtacggcc ttttgcacca gctgcgaaag
    62461 cgtctggttt tgataacgca ccacggtttc gagcgggaat ttcatcggtg ctgctggcgt
    62521 ggactcctct ggcaatggtt gttctggcgt catctgttta accatcatct ctgcaagccc
    62581 cagacctttg cccgccgtca tctgttgggc aatttgctgg tcatacatac tggtatacag
    62641 acgagtgtgc tcgctgctga acaggccatc ttttggtaaa gcgtcgcgca tgcttttcaa
    62701 catcatctgc acgaacatcc cttccacctg acgggccacc ggacggatat ttgccgccgg
    62761 atcttcgctc gctttcgcct ttagttcgtt gagtgattgc gcatcccagg ccgcacttgc
    62821 cagtagtttg ctgtcgctga tcatcagatg atttccagtt ttgcccgcag acatcccgca
    62881 ctttgcattg attgcaaaat agacatcaga tccatcggcg tagcgcccag cgcattgagc
    62941 gcacgcacca cgttattgag gctggcgctg gaacgtacgc tttgcagcga accgccgctc
    63001 tggcgtaaat cgatctgcgt ttgtggagta acaacagtct gtccaccacc aaacggtgta
    63061 tctggctggc tgacattggc ctgacgatta actgttactg agagatttcc ctgcgctacc
    63121 gcgcagctgt cgagggtcac ttctcgattc atcaccaccg aaccggtgcg cgagttaatc
    63181 actactttag cgtcctgcgg ggtgacatta acctgcatat tctggatatc ggcaaggaaa
    63241 cggacctggg aactgttgcc acttggtacg cgcacctgaa tagtccgcgc atctaacgcg
    63301 gtggcgctgc catatccacg cacgcggttg atggtgtcag cgatttgctg cgccatgctg
    63361 aaatcttcgt cgttaagttg caaattaagg gtattcccga cgccaaactg gctgggcaat
    63421 tcacgttcaa taaccgcacc attggtgatc cgtccaccgt tcagttggtt aacctgaaca
    63481 ctgctaccgc ccgcggaggc tcctgcgccg ccaaccagaa tattgccctg cgccagcgca
    63541 tacacctgac tgtcaacgcc cttaagcggt gtcatcaaca acgtacctcc acgcaagctt
    63601 ttggcattac ccatggaaga aaccaccaca tcgatggttt gcccctgacg tccaaacgga
    63661 ggaagtgacg ctgtcaccat taccacagcg acgtttttta gctgcatatt ggtgcccgtc
    63721 ggaacggtaa ttcccagctg tgagagcatg ttattaagcg tttgtgtggt aaacggcgtc
    63781 tgggttgtct ggtcaccagt gccatccagc cccactacca ggccatagcc aatcagtgag
    63841 ttttgcctta ccccctgaac actggtgaga tcgcgaatac gctcagcctg agccgccgtc
    63901 gtaaccagta gaagaattaa tgcagagaga aatttaatca ccacagcctc acttacattg
    63961 gcgacaggtt aaggaagaaa cgctgcaacc agcccatatt ttgcgcttcg ttaatgtagc
    64021 cattgcctac gtattcaata cgcgcatccg ccacctgagt agacggtacg gtattgctgc
    64081 cgctgatagt gcgtggatta accacgcccg agaagcgaat aaattcggta ccctgattaa
    64141 tggcaatctg tttttcaccc accacatgca ggttgccgtt gaccagtacc tggtcaaccg
    64201 tcaccgtcaa cgtgccgcta aaggtattgc tggcattggc cccgcccttt ccgttgaacg
    64261 tgttaccacc ggaggcttcg acatcggcac gagcgttacc aaacagcccc tgcaaatagc
    64321 gcggcacagt atcaaagcca aaattagttt taccgtcacg gctggcattc gcagaggagc
    64381 ttttgctggc gctgacgttc tcctgcaaca cgatggtcag cgtatcgcca atattgcgtg
    64441 gtcgacgatc ttcaaacagc ggttgatagc catagttaat cggctgagca gactggaaaa
    64501 tagaaccgtt ggcgacgggc gtcggaccgg gaaccggttg tgcactggtc gccccctgca
    64561 ccagcggcgt ggagggtatc caggcgcagc cggttagtga aagcaccaac aagctggaaa
    64621 tggcataagt atgcgcagcg tttttttgca tggctatcat cttcaaaaat cagtaaaccg
    64681 gtgaacctgc caccggttat gccttagagt tgcgtcagtt tttgcagcat ctgatcggtg
    64741 gtggacaccg ctttactgtt gatttcgtaa gcgcgttgca cctgaatcat attgaccagt
    64801 tcttccgcca cgttgacgtt agacgtttca acataccctt gatacagcag tcccgcgccg
    64861 ttcaggcccg gcgtgctttc gttcggtgca ccggaggatt gcgtttcggt gtagaggttt
    64921 tcgccaatgc tctccagtcc ggtatcattc ataaaggtgg tgagattgag ctgcccaacc
    64981 tgaaccggag ctgcctggcc ttgttgggtt acgctgacca cgccatcacg accgatggtg
    65041 atacttaacg cattcgccgg aatggtgatc gctggctgca cctgaaaacc accagccgtc
    65101 accagctgcc cgttctgatc cacctggaaa gagccgtcac gggtataggc tgatgagcca
    65161 tctggcaaca ttacctggaa aaagccctgc cctttaatcg cgacatcttt gctgttattg
    65221 gtctgcgaca gattgccctg gctgtgtaag cgttcagtgg cgaccgggcg tacccccgtg
    65281 ccgatttgta atccggaggg taaggtggtt tgttcggaag actgtgcccc cggctggcga
    65341 atggtttgat aaagcagatc ttcaaacacc gcgcgctgac gcttaaaacc gttagtactg
    65401 acgtttgcca ggttgttggc aatgacgtcc atattggttt gctgggcgtc aaggcccgtt
    65461 ttggcgatcc ataatgaact gatcatataa tgtcctgtag tggattgctg acaagcagag
    65521 cagaagcacg cgacagtgtg aatttcccct caccctaacc ctctccccaa aggggcgagg
    65581 gaatagtccg cgcgctttta ttgcaatgtc atcaatcgtt caattaactc atcgacagca
    65641 gttggttggc acggcctgcg ttatcatcga cgctgctgat caccttcatc tgcatttcaa
    65701 aacgccgcgc gctggcaatc atgtcgctca ttgccgcaac ggcattgacg ttactgcctt
    65761 ccagaacccc cgacatcaca cgcaaggttg gatctgcctg cagtaccggc cctcgcgtgg
    65821 cctgggtttc tgcgcttaaa cgaaaaatgc cgtcgtcacc gcgctgcact tcgctgcccg
    65881 tggctttcac cagtttcaga cgccctactg gcgcaactgt atttgccgga tcgcccggat
    65941 tgagcgccga gattgtgcca tcggcagcaa tcgtgatttc cgccccttcc ggcacagcaa
    66001 ttggccccgc ctcgcctatc accggatgcc cctgaattgt cagttgcccg gtgggatcaa
    66061 cctgaatgct gccattacgc gtataccctt cgctgccgtc agcggtctgc acggccagcc
    66121 atcctgctgc aacgcaacgt ccagcgggcg cgaggtgtaa tccatcttgc cgggcgtcat
    66181 gtctgcaccc ggtgttgacg ccgttaccag cgtgcgcgtg ggcagtgaaa gcccttccac
    66241 cggcactgcg cgcaaggcgt ttaactgcgc gcgaaaaccg ggcgtagagg cattggccag
    66301 attactggcg gttaccgcct gttgattcag cgtctggctg gctgctccca tcgcagtata
    66361 aattgcgtga tccattgagc tatcccgtca gcgattagcg taagttaacc cgcgtgttga
    66421 ggatctggtc ctgggttttg atggtctggg cgttagactg atagttacgc tgggcaacga
    66481 tcatattgac cagttcttta ctgagatcga cgttggacgc ttccagcgca ccgttggtca
    66541 gggtgccaaa gtttcccgtc ccggctgtcc ccaacagcgc cacgccagaa gattgcgtcg
    66601 cagaccagac gttgtcgcct tcggatgcca gaccttcgtt gttggcaaag ttcgccagta
    66661 caatctgccc cagcagttgg gtttgttcgt tggaatagtt gccgacaacc gtaccgtcat
    66721 cattgatttg ataactcacc agatcgcccg gtttgtagcc attctgggtg gttgccacaa
    66781 tgttgttagc gccggtattt tgctgcatgg agttgaggaa gctcagacta aacgtggcgg
    66841 gttctgcacc gttaattgcg ccggttgcga tattattcgc catcgcacca tccactaatg
    66901 tgccattagc attaaattcc agtgttgtcg ctgtcttcgc aatgctgttt ggatcactgc
    66961 tatcctgggt gtagacctgc cagttattat ccccggtctt cacaaaatag acgctcatgt
    67021 catgagcatt accctgactg tcgaaaacag tcaccgaacc ttttttgtta tagctatcca
    67081 cattgctggc gctgaatggc gtaacagtag gaagcggatc actggaattc aggttgatct
    67141 gcatcgacgc cgtggtggta gttttcgctg ccatcagggt attcgggatc gaaatattgg
    67201 tcggattcgc cccttgctga atagtcggcg gcgtaccggt tgccgggtaa cccgtcagct
    67261 gtaaaccttg catattcaac aggttacggt tttcatccag cttaaattgt ccgttacggc
    67321 tgtagaacac cgaaccgttg ctgtctacca gacggaaaaa accgttctgg ctgatagcaa
    67381 cgtccagacc tcgcccggtg ttggtggtcg tgccatcggt aaagtcctga gtgataccgg
    67441 caacttttac ccccagtccc actttcgaac cggcaaacat atcggcaaaa gaggccgtgc
    67501 ctgatttaaa gccgtaggtg gcggagttgg cgatattgtt gccaataaca tcgaggttgg
    67561 tggcggcagc gtttaatccg ctaaccgctt gagaaaaggc catgactgac tcctgaaatg
    67621 tgaaggctca aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat
    67681 ccagcgtatt accgctgttg ccgcggatca caccctgcac cagagcaaac tgcagcggct
    67741 gggcaaccag ttgtgtacca ccgttactgg cgctaatcgc tacattgtaa gaaccgttcg
    67801 gcgcagtgct gccatcagtc aacgtaccgt cccaggtgaa actgtgaact ccggcggtca
    67861 gttcaccaat atcaatggtg cgcacaaccg cgccattttt atcggtgatg gtggccgtaa
    67921 ctttgtctgc cgcctgttgc agctcaacac caaacggcgt ggtcgtggtc acagcccctt
    67981 cttcactgcc ggttcccgca agaacagttg tgccggggat catcacgccg tgaccgatca
    68041 ggttactggc ctgtaacgac tggctgttat caatctgtcc ggaaatagat ccgagcgtgg
    68101 tattgagttt ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac gtcagctcgt
    68161 tgttttccat tggattggtc gggtcctggt ttttcagctg cgccaccagc aaagtcagaa
    68221 aactgctttg taaatctgcg gcgttgctgc ccgtgagcga actactgctg gtggtactga
    68281 cgccggtatt tgtcggatcg gtggtggtta ccgcaatgga catagctttc tcctttattg
    68341 accgagcgta agggttttca gcatcatgct tttcaccgtg ttgagcactt caacattggc
    68401 ctgatagctg cgtgacgccg acatggtgtt aaccatctcc ccgacgacat caacgttcgg
    68461 catttttacg tagccctttg catctgccag cggattacct ggttcataaa ccagtttgtc
    68521 cggggcctga ctttcttaac atcggcaacc tttacgccgc ctgtcgcagc acctggtgca
    68581 gcgttaacct ggaataccac ctgttttgcc cgatatggct gtccatcagg accggtcacg
    68641 ctatcagcat ttgccagatt actggccgcc acgttcaggc gctgggactg ggcagttaac
    68701 gccgacccgg cgatatcaaa aatattcagc agtgccatcc gttaatttcc gctctgtaaa
    68761 acgttcatca tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg gtattgcagg
    68821 ctgttatcgg caaactgggt gacttcctga cgctgggcgc gcagattgag cgcctcttgt
    68881 tgaaaacgta aggcggcgtc gagcttatcg agcatatctc ctccgcaggt atcaaaattc
    68941 tgccatcagc ttaaatgcct ttacgcgcgc gttatcggcg gaataaacgc aaaatgggtc
    69001 gctatttatg ccgttgatgg tcattgcgga caggtacaac tcacgttgta gaaatggctg
    69061 ggggcgaaaa tgctgacaat aaaacgtagc gtggcgatca tcgcgatact gttcagtccg
    69121 ttaagtacgg cgagcaatct cacatcgcaa ttgcacaact tttttagcgc ccaactcgcg
    69181 ggggtaagtg atgaggttcg tgtttctatt cgtacagcgc ccaatctact accgccatgc
    69241 gagcagccat tgctttcgat gagcaataat tcccgcctgt ggggcaatgt gaatgtgttg
    69301 gcacgctgcg gtaacgacaa acgatattta caggttaatg tgcaggccac aggaaattat
    69361 gtggttgccg cgatgcctat tgtgcgggga ggaaagctgg aagctggcaa tgtcaaactg
    69421 aaacgtggac gcctggatac cctgccaccg cgtacggtgc tggatatcaa tcaacttgtt
    69481 gatgccgtta gcctgcgcga tctatcaccc gatcaaccta tccagttaac ccagtttcgc
    69541 caggcatggc gggtaaaagc gggacaacgc gtcaatgtga tcgccagcgg tgatgggttt
    69601 agcgccaacg cagaaggtca ggcgctgaac aatgcagccg tcgcacagaa tgcgcgggtg
    69661 cgcatggtat cgggacaggt agtcagcggc gttgttgatg cagatgggaa tattcttata
    69721 aacctgtaag ctgttaaaga ttacccgtcc cttgccgata aataagcaac acatgataaa
    69781 agcgccctca atgaggaata aaccatgagt attgatcgca cttcgcctct gaagcctgta
    69841 agcaccgttc aaccgcgcga aaccactgac gcgccggtaa cgaacagccg ggcggcaaaa
    69901 acaaccgcct ccaccagcac cagtgtgacg ttaagcgacg cgcaagcaaa actgatgcaa
    69961 cccggcagca gtgatatcaa tcttgaacgt gtcgaagcgt taaaactggc gattcgtaac
    70021 ggtgaactaa aaatggacac cggcaaaatt gccgatgcgc tgatcaacga agcgcagcaa
    70081 gacttgcaga gtaactgatc gtatgacacg tcttgcagag atcctcgacc agatgtccgc
    70141 tgtgcttaac gatctcaaaa cggtaatgga tcatgagcag caacatctct ctatggggca
    70201 gatcaacggc agccagttgc aatggattac agaacaaaaa agctcactgc tggcgacgct
    70261 ggattacctc gaacagttac gcaagaaaga acccaataca gcaaatagcg ttgatattag
    70321 tcaacgctgg caggaaatta ctgtgaaaac gcagcaacta cgccaaatga atcaacataa
    70381 cggctggtta ctggaaggac agattgagcg caatcaacag gcgctggaaa tgttgaaacc
    70441 gcatcaggaa ccgacgctat atggggcgaa cggtcagacc tcaacacccc atcgcggcag
    70501 taaaaagatt tcgatctgaa tattatctcc ggcctgcact gcaggccgga atgcattgtt
    70561 acaccgtccg gcgggcaaat tctttaactt tgaagcccag taccgccagt gcagcgaagt
    70621 acgcggcaat ccccgccagc acgaccgcca ttaaacgcag taagcgccag ggcatggtac
    70681 ccaatgacca ctccggcatg atatgtaaca tacctaaaag cacgccagac atcaccagta
    70741 ccgccaccac cagacgcaac agaaacgcca tccagccggg ttgcggagta aagattttct
    70801 gcttacgcaa ctgccagtaa agcagcgaag cattcagaca cgccgccaga ccaatagaaa
    70861 gtgacagccc ggcatgtttc aacggaccaa taaacgccag gttcatcaat tgcgttaaaa
    70921 tcagcgtaac gatggcaatt ttcactggcg ttttaatgtc ttggcgggaa taaaagccag
    70981 gagccaacac tttcactaca atcaggccaa tcaaacccac cgagtaggca attaacgccc
    71041 gctgggtcat cagcgcatca aacgcggtaa atttaccgta ctggaacagc gaaacggtta
    71101 acggacctga aagaatgccc aacgcaaccg cactcggcag tgccaacagg aaacaaagac
    71161 gcaaccccca gtccatcaaa cggttgtatt catcatgatt gccactggca aaacttttcg
    71221 acagcgacgg cagcaaaatg gtgccaagcg ccacgcccag cacaccggag ggaaactcca
    71281 ttaagcggtc ggcgtaatac atccaagaca ccgaaccgga agcaagaaac gaggcaaaaa
    71341 tggtgttgat gattaaggag atctggctca cagagacgcc aaggatcgcc ggtcccatct
    71401 gtttcaccac gcgcattgct ccagcatcgt ggaagttaat gcgcggcagg accagcatgc
    71461 cgatcttctt caggtgcggt agctgataca ccagctgcag gatgccgccg accgtaacag
    71521 cccacgccag cgccagcacc ggtgggttaa agtacggtgc ggcaaacagc gcgaaaccaa
    71581 tcatgctgat gttaagcagt gttggcgcaa acgccggaat cgagaagcgg ttccacgtat
    71641 tcagaatcgc tcccaccagc gacgccaggg agatcagcaa gatataggga aaggtaatct
    71701 ttagtagctg gctggtcagg gcaaatttgt cagctgtgtc agcgaagcct ggcgcggtca
    71761 ccatgatcac ccacggtgcg gcgagcatgc cagcgaccgt cacaaccgcc agcgcaagtg
    71821 tcagcaggcc agaaacataa gagacaaaga cccgcgtggc gtcttcaccc tgcttacttt
    71881 tatattccgc cagaatcggt acaaatgcct gggaaaatgc cccttcggca aagatacggc
    71941 gtaacaagtt aggaagttta aaagcgacga aaaaggcgtc ggttgccatc cctgcgccaa
    72001 agattctggc gacaattgcg tctcgtgcga agccaagcac acgcgaaaac atggtcatcg
    72061 agctgacggc ggccagcgat tttaataaat tcatcggtgt tctaatccgg acccacgggc
    72121 aaaacgcctg caaggcaggc gctgaaagag gcgaatagtc tacctagatt atggtgaatt
    72181 gctaccgcca gatgttacag ggtttattca ctcatcgcat cgcgccagat cttgtcaacg
    72241 atacgttgcg ccagcactgc ctattcgccg gcggtttgcg gaactgtctg attttgcacg
    72301 cattcaatga agtgccgcgc acagccgaca aatccacgtt gctcaagagt gctctgccaa
    72361 ccaggaatcg gtttattcac tacaccctgc ccgcgctcct cacgccattc gcgcatatcc
    72421 gtaatgtcga tgagcgcacc gtcagtcacg gcctgcacgg tttcacgctg acttccggca
    72481 cggcgatgca tacaagtggt gatctgcaaa ggaccggccg aaaaatggtg ctcggcaaac
    72541 agcatttcgc cagcgtcgtt agtcagtagc gtaccgccat ccagagaggc tttgccgccc
    72601 gacaaccaca gcgcggtatc caccacatgc agataatcat ccagcaacgt gaaataaaga
    72661 tcgtgtggcc cgacgctatt gctacgatgt ttatccattc ttagcgaggc tgcggtggcg
    72721 agttgcgttt ttaactcacc gtagagtggt gcgaaacgac ggttaaaacc gaccatcaac
    72781 gtcagttttt tccgcgccgc cagttccacc agcctttcag catcgcgcag attttctgcc
    72841 agcggtttat cgacacagac atgtaccccc gcattgagta acgtactgac cacgtcaaag
    72901 tggctggcgg tgctggagtg cacaaaaacc gcatcacaac tggcggcaag gctggataac
    72961 gaatcggcat aaggaatgcg ccagctgtca caaattggca gggctttggc gcgcgtaggc
    73021 gaccaggctc cttgtaacgt ccagtcagac gctgccgcca gcaccggtaa ccacgctttt
    73081 tgcgcaatgc cgcctaatcc cactacgccg atacgtaatt ttttcacttt aatctcccag
    73141 atgggccagc aacgaatcaa gacgttgttt cagttctgcc acttcgattt ccagggcttc
    73201 gacgcgggtc tgtaaatcac cgtcaaccat gttcgacata gccgttaccg ccggctgatc
    73261 ttcaacctca ccactgaaaa gatgcatgta gcggctttcg cgtttacccg gttcgcgggc
    73321 cagacgcacc acaaaaggac catcttcgcg attcgccagt tgttccagcg tcgactccac
    73381 ttccgccata tcgctgaatt catacattcg cgcggcgcgg ctgcgcagtt cacccagcgt
    73441 ctgggcacca cgcaataaca acgtggtgat taacgccact tctgcggcgc tcagtttcag
    73501 atcgccaaat tctgaattac aaaaacgttg ctcatatttg gtgacccgat taccaaaacc
    73561 gctcactgtg cgtagataat gacgtttgac cagattatcc agctgttcct gcacttcgga
    73621 ttcactcaga ttcatgaccg gttcacggtt cgttttctga ttacaggccg tgactacacc
    73681 attgactgag agcgggtatt gctccggcgt ggtcacctgc ttttccagca agcaaccaat
    73741 tacgcgagct tccagggcag taagttgata tttcatgaga ttctccttag cggccgggcg
    73801 tccagtctgg ggtagttaat gccgtcagta cgtgatcgcg ccattgtcca tcaatcaaca
    73861 gatagtcttt cgcatagcct tctttttcaa aacccagtcg cgccagtaaa tcaccgctgc
    73921 gtttattgtg cggcatataa ttagccataa tgcgatgaat atgttgggtg cgctgcatat
    73981 aacgaatggc tgcggtcagg gcttcaaaca tgagtccttt gccctgccat ttttgcccaa
    74041 tcgaataacc gagatagcag gtatgaaaag agccgcgaac aacattggaa aaattggcaa
    74101 cgccaataat ctctttttcg tccgggtcga ataagccaaa gtagaaagct gaaccttgtt
    74161 tatgaaattc gttaatcatc cctagcctgg cctgccagcc tgatggataa cagtggcttt
    74221 cgtcgcgcac tggctcccag ggcttgagga aatggcgatt ctctgcgtaa taatccgcca
    74281 gacgccaggc atcacgatca tgcaccagac gcacgaccag tcggtctgtg gttaagcgca
    74341 cttttggcac gttactgcga tagccaaaca ttctatacct actccttccc gtaacgtctt
    74401 taatacgctg taagtattac tatacctgcg caaccagacc ctgtgaaaag tccaaaatgc
    74461 cattttctgc attattcaca tatttgataa aaactctcta tcaaagcgac gttttgataa
    74521 aaaaatattg tccccgaagt gatgggaaaa gtcttagcgg caatgcagaa tagaaagtgc
    74581 tcatctttta tttcccggga ggggaaatgt cccgcgtatc gcaggcgagg aacctgggta
    74641 aatatttcct gctcatcgat aatatgctgg tcgtgctggg gttctttgtt gtcttcccgc
    74701 tgatctctat ccgcttcgtt gatcaaatgg gctgggccgc cgtcatggtc ggtattgctc
    74761 tcggtctacg ccagtttatt cagcaaggtc tgggtatttt cggcggcgca attgccgacc
    74821 gctttggtgc caaaccgatg attgttaccg gtatgctgat gcgcgccgcc ggattcgcca
    74881 caatgggtat cgcccacgaa ccgtggctat tgtggttttc atgcctgctc tcgggactcg
    74941 gcggcacgtt gtttgatccg ccgcgttcgg cgctggtggt gaaactaatc cgtccacaac
    75001 aacgtggtcg ttttttctcg ctgttgatga tgcaggacag tgccagtgcg gtcattggcg
    75061 cattgttggg gagctggctg ttgcaatacg actttcgcct ggtctgcgcc acaggggccg
    75121 ttttgtttgt gctatgtgcg gcgttcaatg cgtggttgtt accggcatgg aaactctcca
    75181 ccgtacgcac gcccgttcgc gaaggcatga cccgcgtgat gcgtgacaag cgttttgtca
    75241 cctatgtcct gacgctggcg ggttactaca tgctggctgt acaagtgatg ctgatgctgc
    75301 caattatggt caacgacgtg gctggcgcgc cctctgccgt taaatggatg tatgccattg
    75361 aagcgtgtct gtcgttaacg ttgctctacc ctatcgcccg ctggagtgaa aagcattttc
    75421 gtctggaaca ccggttgatg gctgggctgt tgataatgtc attaagcatg atgccggtgg
    75481 gcatggtcag cggcctacaa caacttttca ccctgatttg tctgttttat atcgggtcga
    75541 tcattgccga gcctgcgcgt gaaaccttaa gtgcttcgct ggcggacgca agagctcgcg
    75601 gcagctatat ggggtttagc cgtctgggtc tggcgattgg cggcgctatt ggttatatcg
    75661 gtggtggctg gctgtttgac ctgggcaaat cgacgcacca gccagagctt ccgtggatga
    75721 tgctgggaat tattggcatc ttcactttcc ttgcgctggg ttggcagttt agtcagaaac
    75781 gcgccgcgcg tcgtttgctg gaacgcgacg cctgatttga tacacccccc tgactcctcc
    75841 atactgaaag tgaactgaca gccaaatgga ggagtcacgt gaagctatac atttacgatc
    75901 actgccctta ctgcctcaaa gcccgcatga ttttcggcct gaaaaatatc cccgtcgaat
    75961 tacatgttct gctcaacgac gacgcagaaa cacccacccg gatggtcggt caaaaacagg
    76021 ttcccattct gcaaaaagat gacagccgct atatgccaga aagcatggat atcgttcact
    76081 atgtcgataa actcgacggc aaaccgttac tgaccggcaa acgttcccct gccattgaag
    76141 agtggctgcg caaggtcaat ggctacgcca acaaactgct gttgccgcgt tttgccaaat
    76201 cggcatttga tgagttttct actcccgccg cgcgcaaata tttcgtcgac aagaaagagg
    76261 ccagcgcggg taattttgcc gacctgctgg cccactctga cggtctgatt aagaatatca
    76321 gcgatgattt gcgtgcgctg gacaaactga tcgtcaaacc gaacgccgtg aatggcgaac
    76381 tttcggaaga tgatattcag ctatttccgc tactgcgtaa tctgacgctg gtagccggaa
    76441 tcaactggcc aagccgcgtt gctgattacc gcgataatat ggcgaaacag acacaaatca
    76501 atttgttatc atcaatggcg atttaatcct gacccgccgc ggcagcctgt attcgcgggt
    76561 tgccgccgtt ttcaattttt tctgctggcg agtttatcgc tggtgacaca tgctgcgttg
    76621 tgtcaggata ttgacgcaga tttcatgtcg aggactacca tgaacaagtt tttatttgcc
    76681 gctgcattga tcgtcagtgg cctgctcgtt ggctgtaatc aactcaccca atacaccatc
    76741 accgaacaag aaattaacca gtcgcttgcg aaacataata atttctcaaa agatatcggt
    76801 ttacccggcg tggctgacgc ccatattgtt ctgacaaact tgaccagcca aattggtcgc
    76861 gaagagccga ataaggttac cctaaccgga gacgccaatc tggacatgaa ctccctgttc
    76921 ggtagtcaga aagcgaccat gaaactgaag ctgaaagcgc tgcccgtgtt tgataaagaa
    76981 aaaggtgcga tcttcctgaa agagatggaa gtggtcgatg cgacggtaca accggaaaaa
    77041 atgcaaacgg tgatgcaaac gttgcttccc tatttgaacc aggcattacg caattacttt
    77101 aaccagcaac ctgcttacgt cctgcgcgaa gatggcagtc agggcgaagc aatggcgaaa
    77161 aaactggcga aaggcattga agtgaagcca ggcgaaattg tcattccgtt tacggattaa
    77221 tgacgagggc gcattcgcgc cctttatttt tcgtgcaaag gaaaacattt ccgcttatcc
    77281 tttgtgtccg gcaaaaacat cccttcagcc ggagcataga gattaatgac tgcaccatcc
    77341 caggtattaa agatccgccg tccagacgac tggcaccttc acctccgcga tggcgacatg
    77401 ttacaaactg tcgtgccgta taccagcgaa atttatggac gggctatcgt aatgcccaat
    77461 ctggctccgc ccgtgaccac agttgaggct gccgtggcgt atcgccaacg tattcttgac
    77521 gccgtacctg ccgggcacga tttcacccca ttgatgacct gttatttaac agattcgctg
    77581 gatcctaatg agctggagcg cggatttaat gaaggcgtgt tcaccgctgc aaaactttac
    77641 ccggcaaacg caaccactaa ctccagccac ggcgtgacgt caattgacgc aatcatgccg
    77701 gtacttgagc gcatggaaaa aatcggtatg ccgctactgg tgcatggtga agtgacacat
    77761 gcagatatcg acatttttga tcgtgaagcg cgctttatag aaagcgtgat ggaacctctg
    77821 cgccagcgcc tgactgcgct gaaagtcgtt tttgagcaca tcaccaccaa agatgctgcc
    77881 gactatgtcc gtgacggaaa tgaacggctg gctgccacca tcactccgca gcatctgatg
    77941 tttaaccgca accatatgct ggttggtggc gtgcgtccgc acctgtattg tttacccatc
    78001 ctcaaacgca atattcacca acaggcattg cgtgaactgg tcgccagcgg ttttaatcga
    78061 gtattcctcg gtacggattc tgcgccacat acacgtcatc gcaaagagag cagttgcggc
    78121 tgcgcgggct gcttcaacgc cccaaccgcg ctgggcagtt acgctaccgt ctttgaagag
    78181 atgaatgctc tgcagcactt tgaagcattc tgttctgtaa acggcccgca gttctatggc
    78241 ttgccggtca acgacacatt catcgaactg gtacgtgaag agcaacaggt tgctgaaagc
    78301 atcgcactga ctgatgacac cctggtgcca ttcctcgccg gggaaacggt acgctggtcc
    78361 gttaaacaat aaaaatcgta gcttcctgtt gtcattaggt tattttacct gtataaataa
    78421 ccagtatatt caacaggggg ctattatgcg aattgaagtc accatagcga aaacttctcc
    78481 attgccagct ggggctattg acgccctggc tggcgaactt tcccgccgta ttcagtatgc
    78541 gtttcctgat aatgaaggcc acgtatcggt acgttatgcc gcagcgaata atttatcggt
    78601 tattggcgca acaaaagaag ataaacagcg cattagcgaa attctccagg aaacgtggga
    78661 aagcgccgat gactggtttg tcagcgaata atatgcagtg atttttgttt ttgccgggtc
    78721 gccccggctt ttttatttta cttacgaatt ttgagtacaa aatttctaca cttcctcaaa
    78781 ataattgcac aatatgctaa aaaaatatga atgacatgct gttttattgt tcatataaat
    78841 cttaaaagca gaaatttatc tgttcccccc tttaaaaatt cggtgaatac ccttacttat
    78901 tggtatactg aagttgcttc agataaaaac gcattgaacc tcgaataacg ttgtctagta
    78961 acacgaatta gggggccatg atggaaaaaa ataatgaagt cattcagact catccgctcg
    79021 tagggtggga catcagcacc gttgatagct atgatgcgct gatgttgcgt ttgcactacc
    79081 agaccccaaa taagtccgag caggaaggga ctgaagttgg tcagacgctc tggttaacca
    79141 ctgatgttgc cagacaattt atttcgatat tagaagcagg aatcgccaaa attgaatccg
    79201 gtgatttccc ggtaaacgag tatcggcgtc attaacatga catgaccatc cacccaaaag
    79261 gcacctcacc ggtgccttta ccatttctgg gttgtatatc tgcattcagt taattactct
    79321 gcttaaacca tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt attggcagcg
    79381 gttccgtagg cgctgccgcc gggtattatg caacccgcgc cggtttaaac gtgctaatga
    79441 ccgacgccca tatgccaccg catcaacacg gcagccacca cggcgatacg cgattaattc
    79501 gccatgctta tggtgaaggc gaaaagtatg tcccgttagt cctccgcgcg caaacgctgt
    79561 gggatgaact ttcccgccac aacgaagatg atcccatttt tgtacgctct ggtgtcatta
    79621 acctcggccc ggctgactcc gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc
    79681 aactcaacgt tgaaaagctc gatgcgcaag ggattatggc ccgctggcca gaaatacgcg
    79741 tcccggacaa ctacatcggc ttatttgaga ctgattccgg ttttttgcgc agcgaactgg
    79801 cgattaaaac ctggatccaa ctggcgaagg aagcgggctg tgcgcaactg ttcaactgcc
    79861 cggtcaccgc aattcgtcat gacgatgatg gcgtaactat tgaaacggtt gacggtgagt
    79921 atcaggcgaa aaaagcgatt gtctgcgcgg gaacatgggt aaaagacctg ctcccggagc
    79981 tgcctgtcca gcccgtacgt aaagtatttg cctggtatca ggccgatggc cgctatagcg
    80041 tgaagaataa attcccggcg tttaccggtg aactgcccaa tggcgatcaa tattatggtt
    80101 ttccggcaga aaacgacgcg ttgaagattg gcaaacataa cggaggccag gttatccatt
    80161 cagcggatga acgtgttccg tttgcggaag tggccagtga tgggtcagaa gccttcccgt
    80221 tcttgcgcaa tgtattgccg ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg
    80281 ataattcgcc tgacgaagat tttattatcg ataccctacc cggccacgat aatacactgc
    80341 tcattaccgg cctgagtggg cacggtttta aatttgcgtc agttttaggg gaaatagctg
    80401 ccgattttgc gcaagaccaa aaaagcgatt ttgatttgac gccattcagg ctttcccgct
    80461 tccaataatc atcattacgg cctccagaat gaggccgttt ttacaggagt atttaatgcg
    80521 tcggctgttg cactatctca tcaataatat tcgcgagcat ctgatgcttt atctttttct
    80581 ctggggattg ttggcaatta tggatctgat ctatgtgttt tatttttagt tttccgcact
    80641 gacataaact gacaaatatt taatctccct ttcatttagc gtgaaaaatt gaatgagatt
    80701 attcctgctt ctacagcact caccagacat ctctgatgct gaacccacta aacttatgaa
    80761 gattctttca ttattaattc aaaattattg aaataaaaat aaatatcatt caatttgtta
    80821 tttttgtgtt gcaattcatt ctccgacacg ccattttctt tctctcgcaa attcgtattg
    80881 gatgcactta tgtcattcac aaatacccct gaacgctatg gtgttatatc tgcggccttc
    80941 cattggctaa gcgcaattat tgtctacggc atgtttgctt taggtctgtg gatggtcacg
    81001 ctcagttatt acgatggctg gtatcacaaa gcacccgaac tgcataaaag catcggtatt
    81061 ttgttaatga tggggctggt tattcgcgtt ctgtggcgtg tcatatctcc accgccgggt
    81121 ccgctgccga gttattcgcc catgactcgt cttgccgcaa aagctggaca tctcgcattg
    81181 tacctattgc tgttcgccat cggcatcagc ggctatctga tctcaactgc cgatggtaaa
    81241 ccgatcagcg tttttggctg gtttgacgtc cccgcgaccc ttgccgacgc tggcgcacag
    81301 gcagactttg ccggtgccct gcatttttgg ctcgcgtgga gcgtcgtagt actgtctgtt
    81361 atgcacggat ttatggccct gaagcatcat ttcatcgata aagacgacac tctgaagcgc
    81421 atgctgggaa agtcgtcatc tgactatgga gtataaaaaa tgaaaaaaag cctgcttggt
    81481 ttaaccttcg cgtccctgat gttctctgcc ggttcagcgg ttgccgccga ttacaaaatt
    81541 gacaaagaag gtcagcacgc ctttgttaac ttccgcatac agcatcttgg ctatagctgg
    81601 ttatatggca cctttaaaga tttcgacggt acttttacct ttgatgaaaa aaatccggct
    81661 gccgataaag tgaatgtgac aattaacacc accagcgtcg atactaacca cgccgaacgc
    81721 gataaacatc ttcgcagtgc ggattttctc aataccacaa aatatccaca ggcaacattc
    81781 acctccacca gcgtgaagaa agacggtgac gaactggata ttaccggcga tctgacgctg
    81841 aatggcgtaa ccaaacccgt cacgctggaa gcgaaattaa ttggtcaggg cgacgaccca
    81901 tggggcggta aacgtgcagg ctttgaggcc gaaggcaaaa ttaagctcaa agacttcaat
    81961 atcaagacag atttaggtcc agcttctcag gaagtggatc tgattatttc agtggaaggc
    82021 gtacagcaga agtaagagtg aaatgttgcc ggatggcacg catccggcaa tgatatttat
    82081 tctgttggat caggaatacc cagtgttgta ttcagacgtc cacgagactt attaaagatc
    82141 ttattgccat tttcacgtcc tgcccgacgg cgtcgctgtt cttctggcgg taacgcgctt
    82201 tcttcgcagc aaatctcact acaacaacct ttgtattttt ccgcgcatac tggacactga
    82261 ataaacagca ggtggcagcc atcattttta cagttggtat ggctgtcgca cggcgcgccg
    82321 cactggtggc aatgcgcgat aatctcatcc gatatacgtt cgcccatccg ctcgtcaaaa
    82381 acaaaatttt tgccaataaa acgcaccggc aagccctgct cgcgcgcctt acgggcgtat
    82441 tcaataattc cgccctcgat atgccagact ttgttgaatc cgttatgttt catccaggca
    82501 ctggcctttt cacaacgaat gccgccggtg cagtacatga cgattttttt atctttatgt
    82561 gcctgcatca tctcgactgc ttttggcagc tgctcacgga aggtatctgc cggaatttcg
    82621 agcgcgtttt caaagtgccc cacttcatac tcatagtggt tacgcatgtc gataaatagt
    82681 gcatcgggat cgtcaagcat ggcgttcact tccgccgctt gcagatactc accaacattg
    82741 ctggcatcaa agtgaggatc gtcaataccg tcggcaacaa tgcgatcgcg taccttcatg
    82801 cgcagtaccc agaaggattt cccgtcatca tccaacgcga tattcaggcg taaaccctct
    82861 aaagccgggt cgaaggcata gagctgcgcg cgaaatgttt caacattgct cgcaggtacg
    82921 ctgatttgcg cgttaatgcc ctcatgcgcc agatacactc gcccaaaaac attcagcgcg
    82981 gtaaacagct gatataaagc gtcacgggtc gccttaggat cggcgatgtg gaaatacttg
    83041 taaaacgaaa tggtggttcg cggttcgctc tcagccaaca ttttggcttt taacgcgtcg
    83101 ttggaaatgc ggttgtgtaa cactggcatg gtgtacggtt cctgcgagat gggaaagtaa
    83161 aaatccgcgg catgatatag caattatcga taattaacat ccacacattt tacgctacat
    83221 ttgcgcatta aaaattattt gttatttaca agcgcggcaa tttcgcccag tcttcagcca
    83281 caaattttgg ttgcgggcga aaaaatgcga caatacatac aattgcccgt ataggttgaa
    83341 aaacaggatt gatatgacga atctacccaa gttctccacc gcactgcttc atccgcgtta
    83401 ttggttaacc tggttgggta ttggcgtact ttggttggtc gtgcaattgc cctacccgat
    83461 tatctaccgc ctcggttgtg gattaggaaa actggcgtta cgttttatga aacgacgcgc
    83521 aaaaattgtg catcgcaacc tggaactgtg cttcccggaa atgagcgaac aagaacgccg
    83581 taaaatggtg gtgaagaatt tcgaatccgt tggcatgggc ctgatggaaa ccggtatggc
    83641 gtggttctgg ccggaccgcc gaatcgcccg ctggacggaa gtgatcggca tggaacacat
    83701 tcgtgacgtg caggcgcaaa aacgcggcat cctgttagtt ggcatccatt ttctgacact
    83761 ggagctgggc gcgcggcagt ttggtatgca ggaaccgggg attggcgttt accgcccgaa
    83821 cgataatcca ctgattgact ggctacaaac ctggggccgt ttgcgctcaa ataaatcgat
    83881 gctcgaccgc aaagatttaa aaggcatgat taaagccctg aaaaaaggcg aagtggtctg
    83941 gtacgcacca gatcatgatt acggcccgcg ctcaagcgtt ttcgtcccgt tatttgccgt
    84001 tgagcaggct gcgaccacga ccggaagctg gatgctggca cggatgtccg gcgcatgtct
    84061 ggtgcccttc gttccacgcc gtaagccaga tggcaaaggg tatcaattga ttatgctgcc
    84121 gccagagtgt tctccgccac tggatgatgc cgaaactacc gccgcgtgga tgaacaaagt
    84181 ggtcgaaaaa tgcatcatga tggcaccaga gcagtatatg tggttacacc gtcgctttaa
    84241 aacacgcccg gaaggcgttc cttcacgcta ttaaatctcc catgccggat gcttcagaat
    84301 ggcatccggc attaccacag caaatccccc tgatttagcg ataaaagctc tctggattgc
    84361 gccccctgga agtcgggcgc ataattagtg tgtttatctt ttcttcttat gttcaccgcg
    84421 cctggcgcac caacagcgga ttgctatgtc accctgtgaa aatgacaccc ctataaactg
    84481 gaaacgaaac ctgatcgtcg cctggctagg ctgttttctt accggggccg ccttcagtct
    84541 ggtaatgccc ttcttacccc tctacgttga gcagcttggt gttaccggcc actccgccct
    84601 gaatatgtgg tccggtattg tcttcagtat aatgggttcc taaacacatc attttagata
    84661 ttgaatatga gacgggcaat cagctacatc agattttcaa gtgaacgaca actgaaaggg
    84721 gactctgtac gccgacaatc taagctggtt actgactggc ttgataagaa ccctgagttt
    84781 tactgggatt catccttgag ctttaaagat ttggggaaaa gtgcttttag tggtaagcac
    84841 ctgaaaggtg gtctaggtga ctttttaacc gctatcgaga aagggcttgt taaagctggt
    84901 gatacgctac taatcgagag tttagaccgt ctgtcacgtc aggatattga tattgcttct
    84961 gagctattac gccgtatctt aagggctggt gtagatgtag tcacactgag cgatggagaa
    85021 cattacacac gagaaagttt aaaagacccg ttagccctga ttaaaagcat tctgataatg
    85081 cagcgggcgc atgaggaaat tttgaggaag tctgagcgtg tccaagctgc atggaaccgt
    85141 aaaaaagaac tcatctccga aggtatcaag gtatctaggc gttgtcctgc atggttaagg
    85201 ctgaatgatg accgtcgaac attcacaatc attcctgata aagtggaagt tgttaaacga
    85261 gcatttgacc tgagattaca gggtttaagc ttttgggcta tcaccagaac tttaaacgat
    85321 gaaggtcact tgtcattgaa tcaatacaca cccaaacaaa aggggtggtc tgatactgct
    85381 gtaaagaaac tactcagaaa tcgtgctgtg attggatgtt tcacacctgc tggacgtgaa
    85441 gaagttcagg gttactaccc cgcaataatc agtgagtcac tattctatcg agtacagcaa
    85501 ctgaacacag ggcaatatgg tagggcttct gtatcaagta atccgctatc agtcaacttg
    85561 ttcaggggta tcatcaaatg ttctga
//
LOCUS       DAJTYG010000003        82537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTYG010000003 DAJTYG010000000
VERSION     DAJTYG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 82537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.003"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..368)
                     /locus_tag="OO568_000229"
     CDS             complement(<1..368)
                     /locus_tag="OO568_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCR6639728.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLG"
     gene            complement(439..2553)
                     /gene="fusA"
                     /locus_tag="OO568_000230"
     CDS             complement(439..2553)
                     /gene="fusA"
                     /locus_tag="OO568_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6639729.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRIGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2650..3120)
                     /gene="rpsG"
                     /locus_tag="OO568_000231"
     CDS             complement(2650..3120)
                     /gene="rpsG"
                     /locus_tag="OO568_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6639730.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3217..3591)
                     /gene="rpsL"
                     /locus_tag="OO568_000232"
     CDS             complement(3217..3591)
                     /gene="rpsL"
                     /locus_tag="OO568_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6639731.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3717..4004)
                     /gene="tusB"
                     /locus_tag="OO568_000233"
     CDS             complement(3717..4004)
                     /gene="tusB"
                     /locus_tag="OO568_000233"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6639732.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4012..4371)
                     /gene="tusC"
                     /locus_tag="OO568_000234"
     CDS             complement(4012..4371)
                     /gene="tusC"
                     /locus_tag="OO568_000234"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6639733.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4371..4757)
                     /gene="tusD"
                     /locus_tag="OO568_000235"
     CDS             complement(4371..4757)
                     /gene="tusD"
                     /locus_tag="OO568_000235"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6639734.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4757..5479)
                     /locus_tag="OO568_000236"
     CDS             complement(4757..5479)
                     /locus_tag="OO568_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6639735.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5646..6458)
                     /gene="fkpA"
                     /locus_tag="OO568_000237"
     CDS             complement(5646..6458)
                     /gene="fkpA"
                     /locus_tag="OO568_000237"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6639736.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6679..6897
                     /locus_tag="OO568_000238"
     CDS             6679..6897
                     /locus_tag="OO568_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6639737.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6946..7536)
                     /gene="slyD"
                     /locus_tag="OO568_000239"
     CDS             complement(6946..7536)
                     /gene="slyD"
                     /locus_tag="OO568_000239"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6639738.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7631..7831)
                     /locus_tag="OO568_000240"
     CDS             complement(7631..7831)
                     /locus_tag="OO568_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6639739.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7841..9617)
                     /gene="kefB"
                     /locus_tag="OO568_000241"
                     /pseudo
     CDS             complement(7841..9617)
                     /gene="kefB"
                     /locus_tag="OO568_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9617..10168)
                     /gene="kefG"
                     /locus_tag="OO568_000242"
     CDS             complement(9617..10168)
                     /gene="kefG"
                     /locus_tag="OO568_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6639740.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSSIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10299..12212
                     /locus_tag="OO568_000243"
     CDS             10299..12212
                     /locus_tag="OO568_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6639741.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIQ
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQAVTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANNAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12212..13234
                     /locus_tag="OO568_000244"
     CDS             12212..13234
                     /locus_tag="OO568_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6639742.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYLLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13228..13446
                     /locus_tag="OO568_000245"
     CDS             13228..13446
                     /locus_tag="OO568_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6639743.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13500..14369
                     /locus_tag="OO568_000246"
     CDS             13500..14369
                     /locus_tag="OO568_000246"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6639744.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     LLVQRLMEGKKIE"
     gene            complement(14424..14828)
                     /locus_tag="OO568_000247"
     CDS             complement(14424..14828)
                     /locus_tag="OO568_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6639745.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15130..15762
                     /gene="crp"
                     /locus_tag="OO568_000248"
     CDS             15130..15762
                     /gene="crp"
                     /locus_tag="OO568_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6639746.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15813..17903
                     /locus_tag="OO568_000249"
     CDS             15813..17903
                     /locus_tag="OO568_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6639747.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17970..19190)
                     /gene="argD/dapC"
                     /locus_tag="OO568_000250"
     CDS             complement(17970..19190)
                     /gene="argD/dapC"
                     /locus_tag="OO568_000250"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6639748.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPQPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQVLLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19276..19839)
                     /gene="pabA"
                     /locus_tag="OO568_000251"
     CDS             complement(19276..19839)
                     /gene="pabA"
                     /locus_tag="OO568_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6639749.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19871..20473)
                     /locus_tag="OO568_000252"
     CDS             complement(19871..20473)
                     /locus_tag="OO568_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6639750.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEIDVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20463..20630)
                     /locus_tag="OO568_000253"
     CDS             complement(20463..20630)
                     /locus_tag="OO568_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6639751.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20735..21307)
                     /gene="ppiA"
                     /locus_tag="OO568_000254"
                     /gene_synonym="rotA"
     CDS             complement(20735..21307)
                     /gene="ppiA"
                     /locus_tag="OO568_000254"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6639752.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21578..22759
                     /gene="tsgA"
                     /locus_tag="OO568_000255"
     CDS             21578..22759
                     /gene="tsgA"
                     /locus_tag="OO568_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6639753.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFSCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23021..25564
                     /gene="nirB"
                     /locus_tag="OO568_000256"
     CDS             23021..25564
                     /gene="nirB"
                     /locus_tag="OO568_000256"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6639754.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHSSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIARKFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25561..25887
                     /gene="nirD"
                     /locus_tag="OO568_000257"
     CDS             25561..25887
                     /gene="nirD"
                     /locus_tag="OO568_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6639755.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26013..26819
                     /gene="nirC"
                     /locus_tag="OO568_000258"
     CDS             26013..26819
                     /gene="nirC"
                     /locus_tag="OO568_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6639756.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26838..28211
                     /gene="cobA"
                     /locus_tag="OO568_000259"
     CDS             26838..28211
                     /gene="cobA"
                     /locus_tag="OO568_000259"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6639757.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28458..28625
                     /locus_tag="OO568_000260"
     CDS             28458..28625
                     /locus_tag="OO568_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6639758.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28920..30257
                     /gene="frlA"
                     /locus_tag="OO568_000261"
                     /pseudo
     CDS             28920..30257
                     /gene="frlA"
                     /locus_tag="OO568_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30278..31300
                     /gene="frlB"
                     /locus_tag="OO568_000262"
     CDS             30278..31300
                     /gene="frlB"
                     /locus_tag="OO568_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6639759.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31350..32179
                     /gene="frlC"
                     /locus_tag="OO568_000263"
                     /pseudo
     CDS             31350..32179
                     /gene="frlC"
                     /locus_tag="OO568_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            32176..32961
                     /gene="frlD"
                     /locus_tag="OO568_000264"
     CDS             32176..32961
                     /gene="frlD"
                     /locus_tag="OO568_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6639760.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33061..33792
                     /gene="phoB"
                     /locus_tag="OO568_000265"
     CDS             33061..33792
                     /gene="phoB"
                     /locus_tag="OO568_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6639761.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33952..35037)
                     /locus_tag="OO568_000266"
     CDS             complement(33952..35037)
                     /locus_tag="OO568_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6639762.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35049..36353)
                     /locus_tag="OO568_000267"
     CDS             complement(35049..36353)
                     /locus_tag="OO568_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6639763.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHLILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTWLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36365..36718)
                     /locus_tag="OO568_000268"
     CDS             complement(36365..36718)
                     /locus_tag="OO568_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6639764.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36729..37607)
                     /locus_tag="OO568_000269"
     CDS             complement(36729..37607)
                     /locus_tag="OO568_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6639765.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLCQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37604..38830)
                     /locus_tag="OO568_000270"
     CDS             complement(37604..38830)
                     /locus_tag="OO568_000270"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6639766.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38831..39994)
                     /locus_tag="OO568_000271"
     CDS             complement(38831..39994)
                     /locus_tag="OO568_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR6639767.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLKA"
     gene            complement(40078..40440)
                     /locus_tag="OO568_000272"
     CDS             complement(40078..40440)
                     /locus_tag="OO568_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6639768.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40457..41362)
                     /locus_tag="OO568_000273"
     CDS             complement(40457..41362)
                     /locus_tag="OO568_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639769.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLLATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41632..42636)
                     /gene="trpS"
                     /locus_tag="OO568_000274"
     CDS             complement(41632..42636)
                     /gene="trpS"
                     /locus_tag="OO568_000274"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6639770.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42629..43387)
                     /gene="gph"
                     /locus_tag="OO568_000275"
     CDS             complement(42629..43387)
                     /gene="gph"
                     /locus_tag="OO568_000275"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6639771.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43380..44057)
                     /gene="rpe"
                     /locus_tag="OO568_000276"
     CDS             complement(43380..44057)
                     /gene="rpe"
                     /locus_tag="OO568_000276"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6639772.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44075..44911)
                     /gene="dam"
                     /locus_tag="OO568_000277"
     CDS             complement(44075..44911)
                     /gene="dam"
                     /locus_tag="OO568_000277"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6639773.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(45018..46304)
                     /gene="damX"
                     /locus_tag="OO568_000278"
     CDS             complement(45018..46304)
                     /gene="damX"
                     /locus_tag="OO568_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6639774.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46396..47484)
                     /gene="aroB"
                     /locus_tag="OO568_000279"
     CDS             complement(46396..47484)
                     /gene="aroB"
                     /locus_tag="OO568_000279"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6639775.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47541..48062)
                     /gene="aroK"
                     /locus_tag="OO568_000280"
     CDS             complement(47541..48062)
                     /gene="aroK"
                     /locus_tag="OO568_000280"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6639776.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQKLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48463..49701)
                     /gene="hofQ"
                     /locus_tag="OO568_000281"
     CDS             complement(48463..49701)
                     /gene="hofQ"
                     /locus_tag="OO568_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6639777.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQASSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49613..50017)
                     /gene="hofP"
                     /locus_tag="OO568_000282"
     CDS             complement(49613..50017)
                     /gene="hofP"
                     /locus_tag="OO568_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR6639778.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(50007..50447)
                     /gene="hofO"
                     /locus_tag="OO568_000283"
     CDS             complement(50007..50447)
                     /gene="hofO"
                     /locus_tag="OO568_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6639779.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50431..50970)
                     /gene="hofN"
                     /locus_tag="OO568_000284"
     CDS             complement(50431..50970)
                     /gene="hofN"
                     /locus_tag="OO568_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR6639780.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50970..51749)
                     /gene="hofM"
                     /locus_tag="OO568_000285"
     CDS             complement(50970..51749)
                     /gene="hofM"
                     /locus_tag="OO568_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6639781.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51869..54421
                     /gene="mrcA"
                     /locus_tag="OO568_000286"
     CDS             51869..54421
                     /gene="mrcA"
                     /locus_tag="OO568_000286"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6639782.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54586..55146)
                     /gene="nudE"
                     /locus_tag="OO568_000287"
     CDS             complement(54586..55146)
                     /gene="nudE"
                     /locus_tag="OO568_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6639783.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55466..57601
                     /gene="igaA"
                     /locus_tag="OO568_000288"
     CDS             55466..57601
                     /gene="igaA"
                     /locus_tag="OO568_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6639784.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRQRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLVLNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57666..58334
                     /gene="yrfG"
                     /locus_tag="OO568_000289"
     CDS             57666..58334
                     /gene="yrfG"
                     /locus_tag="OO568_000289"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6639785.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58345..58746
                     /gene="hslR"
                     /locus_tag="OO568_000290"
     CDS             58345..58746
                     /gene="hslR"
                     /locus_tag="OO568_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6639786.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIKGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58771..59649
                     /gene="hslO"
                     /locus_tag="OO568_000291"
     CDS             58771..59649
                     /gene="hslO"
                     /locus_tag="OO568_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6639787.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59784..61508)
                     /locus_tag="OO568_000292"
     CDS             complement(59784..61508)
                     /locus_tag="OO568_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6639788.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61887..63509
                     /gene="pckA"
                     /locus_tag="OO568_000293"
     CDS             61887..63509
                     /gene="pckA"
                     /locus_tag="OO568_000293"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6639789.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63585..64937)
                     /gene="envZ"
                     /locus_tag="OO568_000294"
     CDS             complement(63585..64937)
                     /gene="envZ"
                     /locus_tag="OO568_000294"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6639790.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64934..65653)
                     /gene="ompR"
                     /locus_tag="OO568_000295"
     CDS             complement(64934..65653)
                     /gene="ompR"
                     /locus_tag="OO568_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6639791.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65881..66357
                     /gene="greB"
                     /locus_tag="OO568_000296"
     CDS             65881..66357
                     /gene="greB"
                     /locus_tag="OO568_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6639792.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66454..68775
                     /locus_tag="OO568_000297"
     CDS             66454..68775
                     /locus_tag="OO568_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6639793.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNGVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69213..69440
                     /gene="feoA"
                     /locus_tag="OO568_000298"
     CDS             69213..69440
                     /gene="feoA"
                     /locus_tag="OO568_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6639794.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69457..71778
                     /gene="feoB"
                     /locus_tag="OO568_000299"
     CDS             69457..71778
                     /gene="feoB"
                     /locus_tag="OO568_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6639795.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71778..72014
                     /gene="feoC"
                     /locus_tag="OO568_000300"
     CDS             71778..72014
                     /gene="feoC"
                     /locus_tag="OO568_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6639796.1"
                     /translation="MASLIQVRDLLALRCRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72217..73095
                     /gene="rpnA"
                     /locus_tag="OO568_000301"
     CDS             72217..73095
                     /gene="rpnA"
                     /locus_tag="OO568_000301"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR6639797.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            73168..73437
                     /locus_tag="OO568_000302"
     CDS             73168..73437
                     /locus_tag="OO568_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6639798.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            73431..73796
                     /locus_tag="OO568_000303"
     CDS             73431..73796
                     /locus_tag="OO568_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6639799.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPCDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(73797..74585)
                     /gene="bioH"
                     /locus_tag="OO568_000304"
     CDS             complement(73797..74585)
                     /gene="bioH"
                     /locus_tag="OO568_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6639800.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPYFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            74623..75306
                     /gene="gntX"
                     /locus_tag="OO568_000305"
     CDS             74623..75306
                     /gene="gntX"
                     /locus_tag="OO568_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6639801.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            75365..75940
                     /gene="nfuA"
                     /locus_tag="OO568_000306"
     CDS             75365..75940
                     /gene="nfuA"
                     /locus_tag="OO568_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6639802.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            76301..77605
                     /gene="gntT"
                     /locus_tag="OO568_000307"
     CDS             76301..77605
                     /gene="gntT"
                     /locus_tag="OO568_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6639803.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLANCGGAQRIATTLIAKFGKKHIQWAVVLTGF
                     TVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIA
                     TIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSF
                     GVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLN
                     RGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLL
                     MAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDP
                     GFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77650..79734)
                     /gene="malQ"
                     /locus_tag="OO568_000308"
     CDS             complement(77650..79734)
                     /gene="malQ"
                     /locus_tag="OO568_000308"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6639804.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79744..82137)
                     /gene="malP"
                     /locus_tag="OO568_000309"
     CDS             complement(79744..82137)
                     /gene="malP"
                     /locus_tag="OO568_000309"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6639805.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        19785 a        22411 c        20831 g        19510 t
ORIGIN      
        1 cccagcagga tgtgctcacg agtctgcggc atcgggccgt cagtcgcagc aactaccagg
       61 atcgcgccgt ccatctgagc agcaccggtg atcatgtttt taacatagtc ggcgtgcccc
      121 gggcagtcta cgtgtgcgta gtgacgggtc ggggtgtcat attcaacgtg agaagtgttg
      181 atggtgatac cacgagcttt ttcttccggc gcgttatcga tctggtcgaa tgcacgagca
      241 gcaccgccgt aggttttagc caatacggtg gtgattgcag cggtcagagt agttttaccg
      301 tggtcaacgt ggccgatagt accaacgtta acgtgcggtt ttgtacgttc aaatttttct
      361 ttagacacgg ctatattcct tactatagtg ctctcccctt caggagagag cacgggactt
      421 tggtattaac ccttaggctt atttaccacg ggcttcaatt acggcctgag caacgttact
      481 cggcgcttca tcatacttca ggaattccat agtgtatgat gcacgacctt tggtcagaga
      541 acgcagctga gttgcgtatc cgaacatttc agacagcggt acttcagcgt ggatcttaac
      601 gccagtaact tcagattcct gacctttgag cataccacga cgacggctca agtcaccgat
      661 aacgtcaccg gtgttctctt ccggagtttc tacttcaacc ttcatgatcg gctcaagcag
      721 aactggtttc gctttcttaa agccttcttt aaaggcgata gaagcagcca gtttaaacgc
      781 cagttcagag gagtcaacgt catggtaaga accgaagtgc agacgaatac ccatgtctac
      841 taccgggtag cctgccagcg gacctgcttt cagctgttcc tggatacctt tatcaacggc
      901 cgggatgtat tcgccaggga ttacaccacc tttaatgtcg ttgatgaact cgtagccttt
      961 cgggtttgaa cccggctcca gcgggtacat gtcgataaca acatgaccat actgaccacg
     1021 accaccagac tgtttcgcgt gtttaccttc aacatcggta actttctggc ggatagtttc
     1081 acggtaagca acctgcggtt tacctacgtt cgcttcaacg ttgaattcac gcttcatacg
     1141 gtcaacgatg atgtcgaggt gcagttcgcc catacccgcg atgatggtct ggttagattc
     1201 ttcgtcagtc catacacgga aagacgggtc ttctttagcc agacggccca gagccagacc
     1261 cattttttcc tggtcagctt tggttttcgg ttcaactgcg atggagatta ccggctcagg
     1321 gaattccata cgttccagaa tgatcggcgc atccgggtca cacagggtgt caccagtggt
     1381 tacgtctttc agaccgatag cagcagcgat gtcgcccgcg cgaacttctt tgatctcttc
     1441 acgtttgtta gcgtgcatct gaacgatacg accgaaacgc tcacgtgcag ctttcacgga
     1501 gttcagtacg gtatcaccag agttaaccac accggagtaa acacggaaga aggtcaggtt
     1561 accaacaaac gggtcggtag cgattttgaa cgccagtgca gagaacggct cgtcatcact
     1621 tgcgtgacgt tcagccggag tgtctttacc gtcgtccagg ataccgttga tcgcaggtac
     1681 gtcaaccggg gatggcaggt aatcaattac cgcatccagc atcgcctgaa cacctttgtt
     1741 cttgaacgca gaaccacagg ttaccaggat gatttcgttg ttcagaacgc gctgacgcag
     1801 agcacctttg atttctgctt cagtcagttc ttcaccaccc aggtattttt ccatcagctc
     1861 ttcagaagct tcagctgcgg attcgatcag gttctggtgc cattcgttag ccagttcaac
     1921 catgtctgcc gggatatctt cgtattcgaa ggttacgccc tggtcagcgt cgttccagtt
     1981 gatagctttc attttcacca ggtcaacaac accggtgaaa tgttcttcag caccaatcgc
     2041 cagctgcagc ggaaccgggt tcgcgcccag acgggttttg atctggttaa caactttcag
     2101 gaagttcgca cctatgcggt ccattttgtt aacgaacgca atgcgcggaa ctttatattt
     2161 gtttgcctga cgccatacgg tttcagactg cggctgaaca ccaccaactg cgcagtaaac
     2221 cattaccgca ccatcgagaa cacgcatgga acgttctact tcgattgtga agtcaacgtg
     2281 ccccggggtg tcgatgatgt tgatgcgatg cggctcatac tgcttagcca taccagacca
     2341 gaatgcagta gtcgcagcgg aagtgatggt aataccacgt tcctgctcct gctccatcca
     2401 gtccatggtt gcagcgccgt catgaacttc accgatttta tggtttacac cggtgtagaa
     2461 cagaatacgt tcggtagtag tggttttacc ggcgtcgatg tgcgcactga taccgatgtt
     2521 acggtagcgt gcgatgggtg ttgtacgagc catttgtttc ctcgtttatc ttttaggcgt
     2581 tcaatttaag tagcccaaag cgggctgctt actggaagcg cccgcctggt gactaaaact
     2641 ccgaagggat taccaacggt agtgtgcgaa cgccttgttg gcttcggcca tacggtgaac
     2701 gtcttcacgt ttcttaactg cagtaccttt gttttctgca gcatcagaaa gttcgttcgc
     2761 caggcgcaga gccatggatt tatcaccgcg tttacgagca gcttcaacga tccaacgcat
     2821 tgccagagca ttacgacgaa ccggacggac ttcaactggt acctgataag tagaaccacc
     2881 aacgcggcga gacttaactt ctacagtcgg gcgcacgttt tcgagagcta cttcgaatgc
     2941 ttccagttca gatttaccag agcgctgagc cagggtctcc agcgcgctgt atacgataga
     3001 ttcagcagta gattttttac catctaccat caggatattt acaaatttag ccagcagttc
     3061 tgatccgaac ttcggatccg gcagaatttt acgctgacca atgacgcgac gacgtggcat
     3121 ggaaatactc cgttgttaat tcaggattgt ccaaaactct acgagtttag tttgacattt
     3181 aagttaaaac gtttggcctt acttaacgga gaaccattaa gccttaggac gcttcacgcc
     3241 atacttggaa cgagcctgct tacggtcttt aacgccggag cagtcaagcg caccacgtac
     3301 ggtgtggtaa cgaacacccg ggaggtcttt aacacgaccg ccacggatca ggatcacgga
     3361 gtgctcctgc aggttgtgac cttcaccacc gatgtaggaa gtcacttcga aaccgttagt
     3421 cagacgaaca cggcatactt tacgcagcgc ggagttcggt tttttaggag tggtagtata
     3481 tacacgagta catacgccac gtttttgcgg gcatgcttcc agcgcaggca cgttgctttt
     3541 cgcaactttg cgagcacgtg gtttgcgtac cagctggtta actgttgcca ttaaatagct
     3601 cctggtttta gcttttgctt cgtaaacacg taataaaacg tcctcacaca atatgaggac
     3661 gccgaatttt agagcgatgc cgaaaaggtg tcaagaaata tacaacgatc ccgccatcac
     3721 caggccatct ggctggagtg cttaaccgta agtctgacga aatcagtata gtcaatggga
     3781 atgatgtcgt tcaaaatttg accagtcaaa ccgcgggcaa taaggtcttc gttcagggca
     3841 tagaccttaa tgggggcatt acgcagactt tcaaggtagc ggttaccgtc aactgcggca
     3901 gttacgccat cttgcaataa tagcagttcg tctccttcac tgagcagacg cagcagcgca
     3961 gcaaaatccg tcagccaggg tgaacgatgt aacgtgtgca gcataaacgc ctcaaaacct
     4021 caaaataaca tcgtagttgg cgagttcgcg gcgtaaggca tctgcttcga gcggcgtggc
     4081 ttcgacaaca aagggtgtct gcggatctaa cccgcgttcg cgcagtgaag ccgcacaaac
     4141 ccagcactgt tcaatgtcgt acagactcaa caatttaaaa gtggcaatgt aatcacgcgc
     4201 cagcactgca tcgggctttt gtcctgacag cagctgaaaa acgccatcag caataaagaa
     4261 gacggccaga tcgtcagtta atgcggaagt tgccagtaaa gcatctaaac cttcccgacc
     4321 agcagctgta ccatgaggtg cagtagaaaa aacaaacgca attcgtttca tcagaactgt
     4381 accaccctgt cacaggtcag cgaggcttcc gccagcgcgc caagtccgct taaggtaaat
     4441 cccggctgaa ggtttgacga agccagcccc agccttcctg cttcagtttc atcgacgata
     4501 ccgcggcgta acgctgccgc tacgcagata ttcagcgcca caccatgttg cgcattcaac
     4561 tgttgccagc cgcgcacaag gtcaaattcg tcgcttgccg gtgaggtcag ttggttagcg
     4621 ttatagaccc cttcccgata gaaaaagacg ctgcttaact tatggccttc tgctatcagc
     4681 gcctgcgcaa actgaaaagc actactcgcc tgttgcgtac cgtatgctgg cccggtcacc
     4741 acgatggcaa aacgcattac ttatcttgcc cctgaaaatc accgctcttg aactggcgga
     4801 tgtagagata gacagtgtgt ttggagatgt tcaggcggtc agcaacctgg ttgatcgcat
     4861 ctttaatatc gaagatccct ttctcgtaga gattcagcac gatctgacga tttttggcgt
     4921 tattagaaac attgcgatcg gcattcactt cttcgatggt gaactccagc gtttgggtaa
     4981 ccagatcttc aacagaagag gcaaagttga cgcttgaacc gacatccggg gtttctggcg
     5041 gcacaaaagt gctcataatc tgcgagaagg gaacatcaag attcatattg atgcacagca
     5101 gaccaattac acgctgttcg cggttacgaa tcgcgatagt cagggacttc attaatacgc
     5161 cgcttttggc gcgagtaaag tagcatttag aaacgctgct atccgctccc gtcatatcgt
     5221 gcagcatacg tagcgccagg tcagtaattg gcgaaccaat cttccggcct gtatgttcac
     5281 cgttagcaat gcgaatggct gaacatttta gatcctgcaa agagtgcaaa acgatttcac
     5341 agtgggagcc aataagcatc gctaacccgt ccaccaccgc ttcgtaggat ttcagaatat
     5401 caaaatcggt ctggtcgaaa ggacgttgat ccagtaaatc caactcactg gtttcgttgg
     5461 ttaaaagcga cctggacatg aaaaaaaaca ctccttttca ggagcctgtc gttaactttt
     5521 cagggcaggc tcattaatga tgcgggtaac taaattaata cagcggaggt tccgctttcc
     5581 agcactaatt atatccggcc tgtaataaaa aaaccgccgc ctggtcaggc ggcggttctt
     5641 aatgcttatt ttttagcaga atctgcggct ttcgcatcag cttccggctt tgcatcagcc
     5701 ttcggcgctg gtttcacatc cagcagctct acgtcaaaca ctagggtaga attcggtggg
     5761 atccccggaa cacccgcttt gccgtaagcc agttctggtg gaataaccag tttgatctta
     5821 ccgcctttct tgatgttctt cagaccttct gtccaacccg ggataacacc gtccagacgg
     5881 aaagaaagcg gttcaccacg ggtgtaagag ttgtcgaact ctttaccgtc gatcagcgta
     5941 cctttgtagt tcactacaac agtatcgctg tctttcggtg cttcgccttt accggcttct
     6001 actacctgat aaaccagacc agttgaagag gttttcacac ctttctcttt ggcaaatttc
     6061 tcgcggtact ctttaccttt tgcttcgtta tcagccgcgt ctttttccat cttcgcctga
     6121 gcagaagact tcacgcgagc ttcgaatgct tgcagagtct gttcgatctc ttggtcggag
     6181 agtttgctct tatcagcaaa tgcatcctga acaccagcga tcagctgatc tttatccagt
     6241 ttgatgccca gtttttcttg ttctttaaga gagttttcca tgtaacgacc cagcgaagca
     6301 cccagtgcat aagctgattt ctgatcgtca tttttgaacg ctgctttgct gtcagcagtt
     6361 gtagcaggtt ttgcagcttc agcagcaaaa gtgattggtg catgcagggc aacggccatt
     6421 gtggtcgcca gtagcgttac tttaaacagt gatttcatcc atatctccag gatcggggca
     6481 tctcacccca gggttaacta ttatcagaag ggtactataa agcgttgtcg aacaaatcta
     6541 catacagaca cgccctatta tcatctattt tcagactctt tttgtttaaa ttagtttcga
     6601 tgaccgcgaa atgagtgctg tctcgggcag caaagttaag tagaatccgc ggcgaccatt
     6661 cgacaaaaga ggtgaatcat gcaggattta tcattggaag cacgcctggc agagctggag
     6721 agccgactgg cttttcagga aatcaccatt gaagaactga acgtcacggt gaccgctcat
     6781 gaaatggaga tggcgaaact gcgcgatcat ctgcgtctgc tgaccgagaa gttaaaagcc
     6841 agccagccgt cgaacatcgc gtcgcaggct gaagaaacgc caccgccaca ttattgaggc
     6901 gtaaaaaaag cggggattcc ccgctttttt gtcacctttt cggtattagt ggcaaccaca
     6961 accgccgttg cctttaccgc cacagcagcc ttcgccaccg tgttcatgac cgtgatcgtg
     7021 gccatgaccg ccgcagcaac cgtcgtggtc gtgatcgtgg tgatgatcgt gcgcgccgtg
     7081 aacgtggcca tgagccagtt cttcttcggt cgcttcgcga atcgccacaa cttcaacgtt
     7141 gaatttcagg ttctgaccgg ccagcatgtg gttaccatca accactacgt gatcgtcttc
     7201 aaccgcagtg atttcaaccg gtaccggacc ctggtcagtt tcagccagga aacgcatacc
     7261 tacctgcagt tcatcaacgc ccataaatac gtctttagga acacgttgca ctaggttttc
     7321 gtcgtactga ccgtaagcgt cgttcgcgcc aacagcgaca tcaaatttgt cgccaacttc
     7381 atgaccttcc agcgccgttt ccaggccaga gatcagggaa ccgtgaccat gcaggtagtc
     7441 cagcggcgca ctcaccggag actcatcaac caacacaccg tcttctgtac gtacctgata
     7501 ggccaggctg accaccaggt cttttgctac tttcatgata tctcctgagc atgggaagaa
     7561 tagtggcgca gattgtagcg gaattctgca gccgcgtact cactagctta aaaaaactcg
     7621 gcgcatatcg ctagtccgga tgaaaaatcc cgatcacttg ctcatctttg tgaacgtgat
     7681 cgcgggcttc tttgtctgct tctcgcatct gatgtccgca cttaacacat tcaacaatat
     7741 caatattatt ttcgcgccac atcgccattg aatcctgcgc ctgacaggcc gggcattttg
     7801 cgcccgctat aaaacgtttt cggattgcca tccttaccct ctactcaaat tcatcccagc
     7861 cgtccagctg gcgtcgttct tgttgcattt cacgctggaa aatctcttcc agttcgcgtc
     7921 gggcttccct ggcgcgagaa atttgtacgg tatcagcatg catcgggatt aactcccgca
     7981 gcattcgcat atccaggcgg cgaaaatgca gttgcgcccg ctgtgcctga tgcggatgca
     8041 tgccaagcgt gaccagcgtc ttgcgcccca gctctaacgc actggagaat gtttcacggg
     8101 aaaactgcgt cacccctgcc tgtaataact catgcgcttc cacacgtccg cgcactcgcg
     8161 caagaatatg caaatgcgga aagtgctgct ggcatatttc caccagcttc atggtgtctt
     8221 ccggctcgtt acaggtaatg acgatagact cagcggcctc tgcacccgca gaacgtaaaa
     8281 gatcgacctg cgtggcgtcg ccgtaataaa ctttgtagcc atatttacgc atcaggttaa
     8341 cggcgctgat atcccgctcc agcacggtaa tgcgcatttt atttgccatc agcaaacgac
     8401 caatcacctg accaaaacgc ccgaagccca cgacaatgac ctggggttta tcatcgttga
     8461 cccacggttt ttcgtcttct tcttccggtc cgttaaactg gcgggatagc catttatcca
     8521 ccagcttcat cagcaacggc gtggtcatca tggaaagcgt caccgtcacc agcaacagcg
     8581 ccatctggtc gccctggaat aaacgttgtg aagaagcggt agaaaagagg acaaaggcaa
     8641 actcaccgcc ctgactcaac acgccagcaa actgcatccg ctctgagcta cgcacgccat
     8701 acaatcgcgc cagcagatac agcacgagaa ttttcaccgc caccagcaca accacgctta
     8761 tcactaccca caacagatgg gtataaagca ccccgaggtt gagcgacatg ccgacagaga
     8821 taaagaacaa accgagcagc aagcctttga agggatcgat agccgtttcc agttcatggc
     8881 gatattcact ttccgccagc agcacgcccg caataaatgt accgagcgcc atcgacagcc
     8941 ccagcgcatc cataaacaat gcggaaccca acaccagcag cagcgtcgcg gcggtgaaca
     9001 cttcccgcac gccagaagct gcaataaagc ggaataccgg acgcagtaaa tagcgcccac
     9061 caatcagcat gccgacaaac gccagcacct tcatgccgat cttcatccag tcgaaatgtt
     9121 cgtctgccga ccccgccaac aacggcacta acgccagtgc tgggatcact gccagatcct
     9181 gaaacagcag aaccgaaaac ccgagctggc cggattcgct gcgattcatc cctttctcac
     9241 gcatcaattg caacgccatt gcagttgaag acatcgcaag gccaatgcca ccgaccaccg
     9301 ccgcctgcca ggcgaaatcc gtcagcatca ataatcccgc cagcaacgcc gcgcttaaca
     9361 gcacctgtgc cacgcctacg ccaaaaatcg aacgccgcag ttgccaaagt ttggagggat
     9421 tcaactcaag gccgatgata aacatcagga ataccacgcc gagttccgaa aagtggagga
     9481 tctcatcgac gtcgctaata aaccccagcc cccacgggcc aattgcaatc cctgccagca
     9541 aatatcccaa cacagcgcca atccccagcc gcgaagagaa acagcactcc tgcgagtaaa
     9601 aaatcggaac cttccatcag cggcctcctg gagacagcgg atttgccaac cagtcaccgt
     9661 aggctctggc gtggctcgcc agctcctttg cgctttgccg tctcgcccag taaataatga
     9721 tggaacttaa ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat
     9781 cgctcatcgg gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg
     9841 taatcacgtt acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac
     9901 cacgacttaa tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa
     9961 gaggatgctg aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat
    10021 caataaaaaa atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg
    10081 tggccggttt aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca
    10141 gcagcaaaac tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag
    10201 tcgctgtttt gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca
    10261 taatgataag ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac
    10321 aaattcgtcg cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc
    10381 agaaagtcgg cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga
    10441 aaaatgaaat cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt
    10501 gggtgaatca ggaaacgcca gcgttaccgc aagcggcgct ggaatatgtc attgacggcg
    10561 accgtgaata tcgtcaactg gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc
    10621 acgccattgc gaccattcat agcaagctgg atgctattga cgcatggagt attcgctccc
    10681 gtgccgccag cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa
    10741 gtgatttctc cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt
    10801 cagacttgct gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc
    10861 tggaaaaatg gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact
    10921 tcctcgatcc gatcgttgat aaaattattc agatcgaaca acaaagcatg ttcgagtaca
    10981 ccggcaacta cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga
    11041 tgtacgaaag ccagcaggaa cgcgtggcac atctgcaaag ttatatcgat cgtttccgtg
    11101 ccaaagccac caaagcgaag caggcccaga gccgcattaa gatgctggaa cgtatggagc
    11161 tgattgcccc cgcacacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc
    11221 tgccaaatcc gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc
    11281 tcgactcgat taaattgaat ctggtccccg gctcgcgcat tggtctgtta ggccgcaacg
    11341 gcgcaggtaa atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg
    11401 aaattggtct ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc
    11461 tgcgcgccga cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac
    11521 aaaaactgcg tgactacctc ggcggctttg gtttcctggg cgataaagta accgaagaaa
    11581 cgcgccgctt ctccggtggg gaaaaggccc gcctggtgct ggcattaatt gtctggcagc
    11641 gtccgaatct gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg
    11701 cagtcaccga agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc
    11761 atttgctgcg ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt
    11821 tcgacggcga tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc
    11881 agaccgacga agcgccaaaa gagaacgcga acaacgccca ggcacgtaaa gatcagaagc
    11941 gtcgggaagc ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg
    12001 aaaaagagat ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca
    12061 gcgaactgta tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca
    12121 gcgccaaatc cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg
    12181 agcagatgct gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa
    12241 tgaatttagc agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca
    12301 aactatgctg ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct
    12361 ggagttgccc gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa
    12421 acataaaccg cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc
    12481 ccacggtctg gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg
    12541 cggatgcagc ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga
    12601 cgccagttgg tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt
    12661 cggctattcg ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct
    12721 cccggttgat gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca
    12781 tatggaaaag ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa
    12841 tgccgcgcgc aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa
    12901 atcggtacgt cgcatccgtg aatttgacga tctaatcacc gccagaattc acggctacgc
    12961 cgacgctatc gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa
    13021 accgacgctg attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccaaa
    13081 accggaaagt ctcccctcgc aggtggagta tctactgact gaacatggcg gtcatgttgg
    13141 ctttattggc ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg
    13201 gttaacaacg tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa
    13261 acgctggaaa atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat
    13321 gagcgtacac ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg
    13381 gtgctggtat ggtcggaact gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc
    13441 gaataacctg caaccgtcgg cggacgagat aatagtgata gctaagtcat ggagttacta
    13501 tgtctgccaa acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca
    13561 ccagcctcgc gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag
    13621 gcgacagttt tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg
    13681 acgccgggcg gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac
    13741 aaaccttcat tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct
    13801 acgacgaagc cgtaccctgg aatcaggtac cgggaacatt caccccctgg caacctttac
    13861 cagaacccac tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc
    13921 ataacgttgc gcagcatgtg gacttactgg tcggcgtagt gcctatcgtt aaccttgagt
    13981 ggattcaaaa actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg
    14041 actcggtagt gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca
    14101 cccatcttaa cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag
    14161 gtatcccgtc gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg
    14221 atttcccttg gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag
    14281 tggtacctgg cggcaaaatg gggctggcaa tggaattaat tatgctgctg ctggtgcaac
    14341 gattgatgga aggaaagaaa atcgagtaac tttgctatta tgccggataa aatactatcc
    14401 ggcttcacaa cgggatagtt aagtcacgcg gcaaccactt catacgagtg agtaatattc
    14461 accgcttttt ccagcatcaa cgccactgaa caatatttct cggcagagag atcaaccgca
    14521 cgcgcaaccg ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc
    14581 gtaaacaggc gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc
    14641 acatcctgac gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc
    14701 gccatcaaca ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa
    14761 atctggtgac cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc
    14821 gcttgcattt ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa
    14881 atcgcgcaac ggaaggcgac ctaggtcatg ctgaagcgag acaccaggag acacaaagcg
    14941 aaagctatgc taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca
    15001 aaggacgtca cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat
    15061 atttcggcaa tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga
    15121 taaccgcgca tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct
    15181 cattgccaca ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg
    15241 gaaacgctgt actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt
    15301 aaagaaatga tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt
    15361 gaagagggcc aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa
    15421 atttcgtaca aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg
    15481 tctgcacaga tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc
    15541 ctcgacgtga cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct
    15601 atgactcacc cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc
    15661 ggctgttctc gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc
    15721 tccgcacacg gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg
    15781 ttacctggta gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg
    15841 atatcaacta tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa
    15901 tgcttggcga attacgattc ggtctgctct tctccctcgt tcctgcctgt tgcaatattg
    15961 cgggccttga tacgcctcat aaacgttttt tcaaacactt aatcattggt gcgtcgctgt
    16021 ttgccacctg tagcttactg acacagctgc tactggtaaa agatgttccc ctgccctttt
    16081 tgctgaccgg attaacgctg gtacttggcg tcactgctga gctggggcca ttgcacgcaa
    16141 aattgcttcc tgcatcgctg ctcgccgcca tttttaccct cagtctggcg ggatacatgc
    16201 cggtctggga accattgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact
    16261 ggttttggtt ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc
    16321 gtgaactggc agattattgt gaagccaaat acagcctgct tacccagcac accgatcctg
    16381 aaaaagcgct gccaccgctg ctggtgcgcc agcaaaaagc ggtcgattta attactcagt
    16441 gctatcagca aatgcatatg ctttccgcgc aaaacaatac cgattacaag cggatgctgc
    16501 gtattttcca ggaggcgctg gatttgcagg aacatatttc ggtcagtttg catcagccgg
    16561 aagaggtgca aaaactggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc
    16621 aaaccgtcgc cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa
    16681 cgcgttttac gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg
    16741 ataatccggt tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca
    16801 cccaaaaacc gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttgc
    16861 ttccggcact gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac
    16921 tcagtgtgat gttaagcgtt gccagcctga tgggcaccgt gctgcatctg ccgaagtcgt
    16981 actggatcct gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga
    17041 ggattgtgaa tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtagcgctgc
    17101 actttaaaat tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct
    17161 acctgatatt gcgcaaaaac tacggctggg caacggtcgg ttttactatt accgcagtgt
    17221 ataccctgca actgttgtgg ctgaacggtg agcagtacat ccttccacgt cttatcgata
    17281 ccattattgg ttgtttgatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga
    17341 gcgggttatt gcgtaaaaac gcccatgatg ctttagaagc ctatcaggaa gcgattcgct
    17401 tgattctgag cgaggacccg caaccaacgc cactggcctg gcagagaatg cgggtaaatc
    17461 aggcgcataa cactctgtat aactcattga atcaggcgat gcaggaaccg gcattcaaca
    17521 gccattatct ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata
    17581 ttaatgccat gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag
    17641 agtatttaca gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg
    17701 aaccgggtag ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag
    17761 gcgcggcagg tacgctggag cagcatttac agcgggttat tggccatctg aacaccatgc
    17821 acaccatttc gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca
    17881 agttgcggga ttcgaaggcg taatacaggc taaaccatcg gataaggcat tcacgccgca
    17941 tccgacatat tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg
    18001 gcgaaacgtt gcatcccctc atcgatatcc gcatcttcca ccaccagcga cggtgctaaa
    18061 cgcatcacat ccggtccggc attcagcacc attacgccaa cctctgcacc cgcatacagg
    18121 aaatcacgcg cccgaccttt gtagtgtggt ttcagctccg cgccaattaa cagccccata
    18181 ccgcgaatat cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa
    18241 cgctggcgtt tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc
    18301 gcgcccgcta ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga
    18361 tgaaacgcag aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct
    18421 aacgctttcg cagaagtcag aatatccggc gtcacaccgt agtacctgta agcaaacaaa
    18481 tcgccagtcc gccccatccc gcactgcact tcatcaaaca ccaataatac ctgatgttga
    18541 tcgcacagct cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg
    18601 ccctggatcg gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgca
    18661 tggagatcgt taaagggaac gtggatgatg tctgccggtt gcggcccaaa gccgtcggaa
    18721 tattttggct gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg
    18781 aaggcaataa ttttggtttt gaacgggcta tgacgcacac aggcgtaatg gcgtgccagt
    18841 ttaaaggcgg tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac
    18901 gtcgcctcaa tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg
    18961 atatgccaca gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg
    19021 cccaacgccg taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc
    19081 cagattcggc tgccctgacc ttttaccgga ataaactctg ccggagcata aatcggcagg
    19141 atcacttcat cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca
    19201 ccctgttacg cataaataaa tatgaaatta taatcacaaa atatgcataa aaaatcactg
    19261 aatggcaatc ggaaatcaga gatgcaggaa attagccagc agttgatgtc cttgttcgct
    19321 aagaatactt tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc
    19381 cataatctcg cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg
    19441 ttccaccacc agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac
    19501 gccctcaccg ctatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac
    19561 aactttgccg ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg
    19621 caagcgcccg gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca
    19681 ggggccaggt gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa
    19741 cgcatcgttg cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt
    19801 ccaggtaaaa gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa
    19861 ccgcgctatt ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca
    19921 gtgcggtgag atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga
    19981 taccctgcca gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct
    20041 gtgccagacc acttcccacc cggaagggat gcagcacgtc gatttcacaa tagtaatgcg
    20101 ccagccgctc gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat
    20161 cctgcatcag ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc
    20221 cctgataaat atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tggtacagct
    20281 ggcgatggat agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg
    20341 cagcacgcag cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggcggatgt
    20401 tcagccggtt acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga
    20461 atttatcgct catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta
    20521 agagtgacta aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta
    20581 cgctgaagct cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc
    20641 cctgaacgtg tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca
    20701 gaagcgcggg caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga
    20761 taactaccgg ttttgacggc acattctggt acggaccaac atcatgagtc ggcacctggg
    20821 aaatcttatc ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat
    20881 cacgctgacc atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg
    20941 cgctgtcttt gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt
    21001 tatcggcttc atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac
    21061 cgccgccctg aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac
    21121 cgctgttcac ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct
    21181 ccagttcgat gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca
    21241 ttgctgcggg agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt
    21301 tgaacatgag atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat
    21361 agccttcaag aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc
    21421 tgacgatcac tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga
    21481 ttgcaataaa acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat
    21541 cccgctatgc cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa
    21601 gctcacatgg attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg
    21661 gatggtgatg ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac
    21721 cttcaccttc ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga
    21781 aatcgtcccg ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc
    21841 cggtttgatg ttcagccaca gcctggcgct gttctcgacg gcgatgttca ttctcggggt
    21901 ggtcagcggc attactatgt cgattggtac attcctgata acacaaatgt atgaagggcg
    21961 tcagcgcggt tcccgcctgt tatttactga ctccttcttc agtatggctg ggatgatttt
    22021 cccaatgatc gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg
    22081 catcgggctg gtgtatgtcg ctatttttat tctgaccttc agctgtgagt tcccggcact
    22141 gggcaaacgt gcaccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt
    22201 actgtttctc tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg
    22261 ggtgcctgag tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag
    22321 taacttctgg atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt
    22381 tgatttgcaa cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt
    22441 taacaccgga acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag
    22501 cgcgatctat accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa
    22561 actggttaac tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac
    22621 cggcccgatt gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta
    22681 cgctgtcgtc tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa
    22741 caccctgacc tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc
    22801 tctcttcccc cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc
    22861 acaaacatga aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaacat
    22921 accccttaag gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat
    22981 cgttaaggta ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc
    23041 aattatcggt aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga
    23101 tgcggccaac tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt
    23161 acacctctcg tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg
    23221 cttctacgag aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg
    23281 tcaggagaag gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat
    23341 ggcaaccggt tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt
    23401 tgtctatcgc accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg
    23461 cggtgccgtt gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt
    23521 aggtattgaa acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca
    23581 gatgggtggc gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcactccag
    23641 caaaaacacc cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc
    23701 cgacggcagc gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga
    23761 taagctggca acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga
    23821 ttcctgccag acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa
    23881 ccgtgtattt ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat
    23941 tctgggtagc gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg
    24001 cgtagacgta ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt
    24061 ttacctcgac gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac
    24121 cctgctcggt gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt
    24181 gctgaacgct atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg
    24241 tagcggtaag ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg
    24301 cttcgacgtc actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc
    24361 ggcgctgaaa gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac
    24421 tcaggtactg aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga
    24481 acactttgct tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac
    24541 cttcgaagaa ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac
    24601 cgtcggttcg ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc
    24661 gctgcaggat tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt
    24721 gatcccgcgt tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat
    24781 cgcgcgtaaa tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg
    24841 cgcacagaaa gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac
    24901 cggtcatgcc tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg
    24961 ccgctacggt gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg
    25021 catccgtacg ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga
    25081 agctcagggt aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg
    25141 cggtaacggc ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga
    25201 aacgctgatc aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact
    25261 gacgcgtacc gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt
    25321 gatcattgac gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg
    25381 tgaagcggta gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt
    25441 caaacacttc atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga
    25501 acagcaccgt ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc
    25561 atgagccagt ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc
    25621 gcgctgttag gtgacgagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt
    25681 gcgatcagca acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg
    25741 gaacaccagg gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt
    25801 gatggcttgt gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa
    25861 gacggcgtgg tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt
    25921 tgcatggtcc tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta
    25981 ttttttgttt ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg
    26041 cggctaatgc tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca
    26101 gctccgccat ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta
    26161 atttgctcga cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa
    26221 cgctggtgat tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg
    26281 gggtaaaagc gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc
    26341 tgggtaacct ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc
    26401 tgctgccggt agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg
    26461 caatggtact gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga
    26521 tggcgctgcg cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat
    26581 ttatcgcgtc cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt
    26641 tcggcaacca cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga
    26701 cgctgggtaa tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc
    26761 cgaaagcgaa tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat
    26821 tactaagggg tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact
    26881 gtctgattgt cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag
    26941 gcgctcgctt aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg
    27001 caggcatgtt aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc
    27061 tggcgattgc agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag
    27121 ctcgtcgcat cttctgtaac gtagtcgatg cgccgaaagc cgccagcttt attatgccgt
    27181 cgattattga ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc
    27241 tggctcgcct gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg
    27301 cgaaatatgc cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc
    27361 gtcgccgttt ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca
    27421 acgatcagaa agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc
    27481 gcggtgaagt ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag
    27541 gactgcaaca aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata
    27601 ttatgaatct ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc
    27661 actgtgtacc gcaggaagag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac
    27721 gcgtggtgcg gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg
    27781 aaacactgtg caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg
    27841 gttgctctgc ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct
    27901 taattaccgg acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa
    27961 aacagacgct ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga
    28021 ttgaacacgg tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca
    28081 cgcagcgcgt gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca
    28141 gtccatcgct aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct
    28201 ccaatcatta atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat
    28261 attcagcaaa taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc
    28321 gttacagcga cttacaaaat taagtcattc gttcactgac gccaattaaa tattcctgta
    28381 tcgttcttaa cgagcagtct gcttatattc atataatcaa tgaatattaa ttaatatata
    28441 atacataggg aatgtaaatg aacaaattta ttaaagttgc actggtaggt gcagtactgg
    28501 ctacgttaac tgcatgtact ggtcatattg aaaaccgtga taagaactgc tcttacgact
    28561 acctgctgca cccggcaatt tctatttcta aaatcattgg cggttgcggt cctactgcac
    28621 agtaagtctc tgacagaaat cggctaacac cggtttaatt gcccgacgcc ctctggattt
    28681 tccgagggcg tatttgtttc tatgtccttc ttgcccccgc ccgtaaaaat attttcactt
    28741 taaattcaat tttataacta catccattag ccacagcgcg atcctgtgcg aaattttgtg
    28801 atcttcctcc acattacaca acatcatatg ttgttatatt catcatgcat tgtcatgttg
    28861 ccttttaaat gactgcaaac tctcccctac aacgtattgg acaagaaaaa ggtatcgcta
    28921 tgggaagcca ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg
    28981 ggacaaccgt cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcagcgggca
    29041 cgccgtggct tacggtgctc gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt
    29101 gcgtctatgc ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga
    29161 aaaatgccgg aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg
    29221 atgcgccgtc attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc
    29281 ctatcgatcc gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgttgc
    29341 tacacctgcg ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa
    29401 ttatcccgtt cactatcgtc attggccttg ggatcttctg gtttaaagcg gagaattttg
    29461 ccgcccttac caccactgcg attggcgcaa cgggcagctt tatggcgctg ctggcgggga
    29521 tctctgccac cagttggtcg tataccggca tggcctctat ctgttatatg accggcgaaa
    29581 ttaaaaaccc cggaaaaacc atgccacgag cgctgattgg ttcctgtctg ctggttctgg
    29641 tgctctacac cctgctggcg ctggtgattt ccggcctgat gcccttcgac aaactcgcca
    29701 attctgaaac gccgatttcc gacgccctga cctagatccc cgcactcggc agcaccgctg
    29761 ggatctttgt tgccatcacg gcgatgatcg tcattcttgg ttcgctttcc agctgcgtga
    29821 tgtaccagcc gcggctggaa tacgcgatgg cgaaagacaa cctgttcttt aaatgcttcg
    29881 gccatgtgca tccgaaatac aacacgccgg atgtctccat catcctgcaa ggggcgctgg
    29941 ggatcttctt catcttcgtt tccgatctca ccagcctgct gggttatttc accctggtga
    30001 tgtgtttcaa aaataccctc accttcggct ccatcatctg gtgtcgtaaa cgcgacgatt
    30061 acaaaccgct gtggcgtact ccggctttcg ggctgatgac caccctcgcc attgcgtcaa
    30121 gcctcattct ggtcgcctca acctttgtct gggcaccgat tcccggcctt atctgcgccg
    30181 tcatcgttat tgctactggt ctgcctgctt acgccttctg ggcgaagcgt agccgccagc
    30241 tcaacgcttt gtcgtaatgt tatctgaaga aaataaaatg ttggatattg ataaaagcac
    30301 cgtggacttt ctggtcaccg aaaatatggt tcaggaagtg gaaaaagttc tcagccatga
    30361 cgttccgctg gtgcacgcca tcgtggaaga gatggtgaag cgcgacattg atcgtattta
    30421 tttcgttgcc tgcggctcgc cactcaacgc ggcgcaaacg gcgaaacatc tggcggatcg
    30481 cttttccgat cttcaggtct acgccatttc cggctgggag ttctgcgata acaccccgta
    30541 tcgcctcgac gatcgttgcg cggtgattgg cgtttctgac tacggtaaaa cggaagaggt
    30601 aatcaaagcg ctggagctgg gccgggcctg cggcgcactc actgcggcgt tcaccaaacg
    30661 cgcggacagc ccgattacct cggcggcgga atttagcatt gattatcagg ccgactgtat
    30721 ctgggaaatt cacctgctgc tctgctacag cgtggtgctg gagatgatca cccgcctcgc
    30781 gccgaacgcg gaaatcggca agatcaaaaa cgatctcaag cagttgccga atgcgctcgg
    30841 tcatctggta cgcacctggg aagaaaaagg ccgccagctt ggtgaactgg ccagccagtg
    30901 gccgatgatt tataccgttg ctgcgggtcc gctgcgtccg ctgggttaca aagaaggcat
    30961 tgtaacgctg atggaattta cctggacgca cggctgcgtg attgagagcg gagagttccg
    31021 ccatggcccg ctggagattg tcgaaccggg cgttccgttc ctgttcctgc tcggcaatga
    31081 tgaaagtcgc cacaccaccg aacgcgccat taactttgtt aaacagcgta ctgacaacgt
    31141 gatcgtcatc gattacgccg aaatttcgca agggctgcac ccgtggctgg caccgttcct
    31201 gatgttcgtg ccaatggagt ggctctgcta ctacctgtct atttacaaag atcacaaccc
    31261 ggatgaacgc cgctattacg gtggtctggt ggaatattaa tccctctccc ggcccggcaa
    31321 cgggccggat ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc
    31381 agcggctgcc tattgaacac gcatttcgtg atgcaagcga gctgggttac gacggcatcg
    31441 aaatttgggg cggtcgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac
    31501 aaatcaaggc gctggcgcag acgtatcaga tgccgattat cggttacacg ccagaaacca
    31561 acggctatcc gtataacatg atgctgggcg atgaacatat gcgtcgtgaa agcctggaca
    31621 tgatcaagct ggcgatatat atggcaaaag agatgaacgc gggttatacg ctgatttctg
    31681 cggcccacgc gggctatctc acgccgccta acgttatctg gggcggctgg cagagaactt
    31741 aagcgaactg tgtgagtacg cggaaaacat cggcatggat ctgatcctcg aaccgttaac
    31801 gccgtatgaa tcgaacgtcg tgtgtaatgc taatgatgtg cttcatgcgc tggcgctggt
    31861 gccttcgccg cgcctgttca gcatggtcga catttgcgcg ccgtatgtcc aggcggaacc
    31921 ggtgatgagt tatttcgaca aactgggcga taaattacgt catctgcata ttgtcgacag
    31981 cgacggggcc agcgacacgc attacattcc tggagaaggc aaaatgccgc tgcgggaact
    32041 gatgcgcgat attattgatc ggggctatga gggttactgt acggtggagc tggtgacgat
    32101 gtatatgaac gagcccagac tctatgcccg ccaggcgctg gaacgctttc gcgcgctgct
    32161 gccggaggat gagagatgaa aaccctagcg acaatcggcg ataactgcgt cgatatctac
    32221 ccgcaactga ataaagcgtt ttctggcggt aatgcggtca atgtggcggt gtactgcact
    32281 cgctacggca tacagccggg atgcattacc tgggtgggtg acgatgacta cggcacaaag
    32341 ctgaagcagg atctcgcccg catgggcgtc gatatcagcc atgtccatac gaaacacggc
    32401 gttaccgcac aaactcaggt ggaactgcac gacaatgatc gcgtttttgg cgactacacc
    32461 gaaggcgtga tggccgactt tgccctgagt gaagaggatt acgcctggct ggcgcagtat
    32521 gacattgtgc acgcggcaat ctggggacat gcggaagacg cattcccaca gctgcacgct
    32581 gcgggcaaac ttaccgcttt cgacttctcc gacaagtggg acagcccgct ctggcagaca
    32641 ctggtgccgc atctcgattt tgcctttgcc tccgcaccgc aagaagacga agcgctgcgt
    32701 ctgaagatga aagcgattgt tgcccgtggc gcaggaacag tgattgtcac gctgggtgaa
    32761 aacggcagca ttgcctggga tggcgcgcag ttctggcgtc aggctcctga accggtgacg
    32821 gttatcgaca ccatgggtgc cggagattcg ttcattgccg gattcctttg cggctggtct
    32881 gcggggatga cattaccgca ggcgatggcg cagggaacgg cgtgcgcggc gaaaaccatt
    32941 cagtaccacg gtgcctggta ggtataacgt tggcgtgagc atcttcacgc caacgtgctg
    33001 ttacttgccg gaaaacgacc ctataatccg agtaattcat tctttatttc agggtcgatt
    33061 atgtcagcta cggaccgcta ctctcatcaa ctcctctacg ctaccgtccg ccagcggctg
    33121 ctggatgata tcgcgcaggg ggtttaccag gccgggcaac agatccctac cgaaaacgag
    33181 ctttgtacac aatataacgt cagccgcatt accattcgca aagccatcag cgacttagtg
    33241 gcagacggcg tactgatccg ctggcaggga aaaggcacct ttgtacaaag ccagaaagtt
    33301 gaaaacgccc tgcttactgt cagtggtttt accgattttg gcgtctcaca aggcaaggcg
    33361 acgaaagaga aagtgatcga acaggaacgg gtcagcgccg cgccgttttg cgaaaagctg
    33421 aacatccccg gaaacagcga agtgttccat ctctgccggg tgatgtatct cgataaagag
    33481 ccgctgttta ttgatagttc atggatcccg ctgtcgcgtt atcctgactt tgatgagatt
    33541 tacgtcgaag gaagctccac ctatcagtta tttcaggagc gttttgacac gcgagtggtc
    33601 agcgacaaaa agaccatcga tatctttgcc gccacccgcc cgcaggcaaa atggctgaaa
    33661 tgcgaactgg gcgaaccgtt gtttcgcatc agcaaaatcg cctttgatca gaatgacaaa
    33721 ccggtgcacg tctccgaact cttctgccgc gccaatcgca tcaccttaac gattgataat
    33781 aaaagacatt aaccgtaggc cggataagat gcgccagcat cgcatccggc gatgctggcg
    33841 cactgaattt tacatcccgc acgttcccct caccctaacc ctctccccaa aggggcgagg
    33901 ggagcgttca gtgcggaatt tagcttgatt gccgaacggt gctatccagc attaaatact
    33961 cgcaatactc tcacgcaaaa tccgcagcac cgtctcttca ccgctgcgat tcgggttaat
    34021 gcgaatcgca caatgttcga gttgtggatt cgcctggcga aacgttcctg aaagtcgata
    34081 gaagagcggc gggatttcat atttcgactc tgcaccgacc gggtaaggca acgcgccacg
    34141 cttttgcgct tcttccagca ccctggcggc aatcagctga tgaaactcga caatcaacac
    34201 cttcgactgc gcattggcaa tgacggcgct tttcacttcc gccaccgcgc cgccgttaag
    34261 caaagccagc aaccgttcgg atactcccgc ctgcaccgca tgcatcactg gcgcgaacac
    34321 cagaccacgc aagacttcca gcgcctgtga gccctggatc tggctaccgc cggagtaaag
    34381 cgtggcgcga atccgactaa taacatcggc atcgccaacc actgcaccaa cgccctccgg
    34441 cccaaacagc ttgaagcagg agaatgtcga gacattcgcg ccgcattcac agccgattcg
    34501 cgctaccttc atcaccgcat agttatcatc ggttaacgct ggaacacctg ccgcgcgcaa
    34561 cgttgccagc acatctgcca gcacatagct gtcctgcggc tgctggcgcg tatgctgcac
    34621 cagcgcggca tcaggttgtt gctcgtcgac gacctgcttc agtgccaaca ggtcattgaa
    34681 atcagcagta ataagcgtca gccccatctg ctcaataata acccgtgtcg tcgggtaaac
    34741 aggcgcgtca tgcaccagaa gacgctgccc cggtttgagc aaagccgcca gcccggcgcg
    34801 aatcgcgcca gtccccgcgc cctgcaccag cgccgcggcc tgtgcgtgaa atgcatcagc
    34861 cagcacctgc tccacccgtt gggtaatacg cggttgattc agacctggag ttaaccctaa
    34921 atcaccgcca gcaagaaact cgctgcctgg aaaatggcgg caaatagtgt ccaccagcgc
    34981 aaactgcttt tgctgcgctt cagcaagagt caggctttgc aaaggaaacg tcttcatagg
    35041 agccttcctt aagccggaac gaacaggcca agccagtaaa gaacattcag cagaataccg
    35101 gtgatcatta ctgccacgac cggtgccgcc attttctgta ccggacggcc cagggattcg
    35161 ttgaggaagt aaatggcaac cgcgatagag aatccggtat aacccgccat cttaattgct
    35221 gcgaaaatcg aaccgaccag cagcgccact tccatcagca tattcatggc gttacggatg
    35281 ttatccgacg cattacgcac cgacgggtag cgtcccagcc atttgccgat cgaacgaagc
    35341 agcaagactt ccgccgaaat aaccactgcg cctaataccg ctgcaaccat cggattcggc
    35401 gagagatagc ccaccgcata aacaaaggta aagcccgcaa ctgcatacac acccgttgct
    35461 aaggcggtgg tggcaatcaa cggcacaaac cccagtccgc gcataaattc tgccagagcc
    35521 gcctgattaa tcagcgtttg cgattgttcc ggcgtcacgc ctgcggaata cgctttctcc
    35581 agtgtgaaga tcgaaacttc gctaccagca aaaatcttca tactggcaac ggcggcaatc
    35641 aatgctccca cgatggcgat ataaggtaag tttttgataa tccgcgacgt gcgctcttca
    35701 aacaccgaaa gcccgctggc atcgatgtca ttttcatcac gatgacgcag gtcgtgagtt
    35761 atcgcgatcc ccagcagcat caccatgcca ataaagattt caatggattc agggttaaga
    35821 tgtgggaaat agcgcacgac taccacacgg gtcatcagaa caaccacggc ggcgaccaga
    35881 ctttgcttcc agccaaactg gtagaaaatc gccaccagcg ggaacagtgc aaaagcagaa
    35941 accaccggcg agcttaattc cccgaggcta cctaatacat ccaccggcag cgcggtcagc
    36001 agctggttaa ctggcaacag gcaagtaagg atcaacacgc cccagatagc gccaagacca
    36061 aacgccatca ggctgtttat cgccagtacg ccgaggatat cggtaggaag aaagagtaac
    36121 catgcgttga gcagccaggt tttcagggta aaagagatcc ccaccgaggc cacaaaacca
    36181 atgctcagac caaaagcgat actccccgcc tcgcgacggt tcatatagcc ttcaatcagt
    36241 tgcggcagga tcaggtggat gccgtcatga aaaacagccg ccgagcgatg cgccagaagc
    36301 gatgtcatac ccgtcaggca cgccaccacg ataatctgaa tatacagatc catagtcgtg
    36361 ccttttattt cagatggtta atcaacatcg gaatcgcgtg ttcgacgtgc tcaacggaaa
    36421 ggccaaacgc cactttccct tcagcgatca ttttggcgat atgctcgtct ttcgctttaa
    36481 tgcctggttt ggcaatggtg caacttttgt tatagccaat caccgcgatg gcaatcgaca
    36541 atgccgcgcc cgcaccggta ttacacgcgc caatgtaata atctagttgc ccggatttca
    36601 ccttcattgc cgcttccatg tcgttgtgaa tgaaaacttc aaaacagcca ggagccgtcg
    36661 cttcaatggt ttttttaatc tgctcacgct gtaagcctgc aacgccaatc tttttcatga
    36721 gtctgtcctt attggaaaaa ttgagaggga ttttcacgta acatcacatc cacatcggcc
    36781 tgactgaatc ctgactggca caattgcgga ataaaggtgg tcaataaata gtcatagcca
    36841 taaccaccat tggcttttaa atgggagcgg cgcgtaatat ccatcgacag catgacgcgg
    36901 ttcagcaacc cacggtcacg tagcgcgtgg agcatcgcaa tgcgcttttc gtccgggtag
    36961 taactgttct taccaatggt gtcgaactgc acgtacgcgc cgagatcgat catcttcaaa
    37021 atgttgtcga ggttgtcttt cagatcgcag tgaccaacgg tgacgcgcga aagatcaact
    37081 ccgtgggctt gcaacagagc cagttgttcc agccccatgg tgctgaacga cgtatgcgtg
    37141 gagatcgggc gtccggtctg gttatgcgcc agcgcagcgg caataaatac cttctcttcc
    37201 agcggcgtaa tctttccttc gctggagccg atctccgcga tgatcccggc tttcagctcc
    37261 gtgccatcga taccctgttc aatttcatcg accatctcct gcgccagttc ctgcacgctg
    37321 cgggtcacca catgttccgg gaaaaacgca tcctggtaat aaccagtaca ggccaccacg
    37381 ttgatcccgg tttcgcgcat tacatcgagc ataaattgcg cattgcgccc catgtaacgg
    37441 ttggtcatct caatcacatt acgtacaccc cgggtcatca ggtcgttcat ctcctggcaa
    37501 atgaacgcat actgatcaag gcggcagtcc acgttgtttt taaagccgga gagatcaata
    37561 tgcagatgct catgggccag ggtgtaaccc gtcggatcaa aactcatagg gtgtctcctg
    37621 caaaccggag ggaggaaaga aaagagcgac cattttgtgg cggtggcgca cggaaaaatt
    37681 cacacacggt agctcccaca tcagaaagcg tggtgcgcac accgagctgc gtggcgacca
    37741 gcccttgctg ataaaccagc actggcacca cttcacgggt atggtggctg tgaccaatgg
    37801 tcggatcgtt accgtgatcc gccatcacga ccaggcaatc atctggctgc atcgcctcaa
    37861 caagccgggc aaggttacgg tcaacgacct gcaaacgttc ggcataacgt gcgacgtctt
    37921 ctgcatgacc agcgaggtcg gtttcctgga tattgatgca aataaacgcc gtcggatggg
    37981 tgttaaattc gtcgagggtg atatccataa tccgctggct atccaccagg ttttgccagc
    38041 tcacaccata aggattgctg acgatatctg ccaccttacc caccagcacg gttggcacgc
    38101 ccgcttcata cagtttttgc ggcacctgca ctttttcatc aacaccgtag cccatatgca
    38161 cgacctggaa gccgttgtca taagcgccag aacgcggcgc attaatacca ataaagcgcc
    38221 cttctttgct ttctgcggca tcgagaatac gctgactgtt ggttaacagg ccgccaaatg
    38281 taatgacccg accgacctgt acctggtcac gcacgatacg accaattttg attgcgtcgt
    38341 caaaagagat cacagagaga ttggcggtaa tgttatagac ttggcctaaa tccgcctcga
    38401 gattatcgcc aatcgcaacc gcctgattga cccacagaaa ttgcagatca tcgccacggc
    38461 gctccacctg ccagccagcg gaaactaatg cctgctcaac acggtcaatc acatcgcgaa
    38521 aaggcatccg cagcggcggt aacgggcgcg tgcctaaaat ttcctgatgc cccataaagg
    38581 tatcgccacc ttcatgttgc agctctgcca cgccccaggt tgcggaatct gacggctgca
    38641 tatcgcctgg cgcataaccc aatgtgttga ttagccccag cttctccaac gttggtagct
    38701 gcaaatgcgg caactggctc aggatgtgac cacaggtatt cgccccggca tcttgcggac
    38761 gcaccagcgt gacatctttc attgccccta cgccaaagct atcaatcact aacaccacaa
    38821 atcgcgccat ttatgccttc agagggttgc cgaggctgtc ataacgacca actatttctg
    38881 gttcgccgcg ctgaataccg gacaccagca ccacatcgct acgggtgaca aaaatctgcg
    38941 tgcgaaaaca gagcaccact gcactgctca ccggatactc gcctgccagc ggcagggtgt
    39001 agtcgatact gctgtcatcc acagttttga gattggtttc agtaatcttt tgattttctg
    39061 gcgtaaacac cagcgcatgt tgcgcatgac cacgacggta gtaaccgccg ccgtagcagt
    39121 agctgtcgcc acggaaatga tgggagattt cacttaacca gagcatcgcg atacgttcag
    39181 gctgatcgcc ctgctggttt gccggaatag taccggtcag cgcatgaccg ggttcggcat
    39241 gagtcgcacc gtattccgcc agcaatggca gcgaagtgca gctggtcgct gaaggtacgt
    39301 tcagttgctc aatcgcaata ccagatttcg ccagttgatc ccgtgcttgt accagcgtgt
    39361 gaagattcgg tgttggcaaa actttcccgg cagcctcatc ccaaagcagg caagggaaat
    39421 gggttagtcc ggctaaatgc agccccggca gtttcttaat ttcagcaatc acctcgtgca
    39481 gcgaatgctg tacaaaaccg ctctcctgcc ccggataaag aaaatcatcg tcgctataaa
    39541 ctttgagcag cacaggctgg acccgtccgg ccttaaccgc tgccgcagaa acttcccgcg
    39601 ctttgtcgag agtaaacacg gtgatgatgt cggtgccctg ttcaacggcg tcagcaacct
    39661 gatgacaagg gatttgtacc agatgcccct gatgcgccac tggcaaacca gcgcggcgca
    39721 tgactcgcgc ctctttgtaa tccaccgcca caatgccgct gtagcctaat gccagcaatt
    39781 tttccgccag ccacggatta cgaccaaatt gcttggtcat cagatacagt tcaatgccgt
    39841 aaagccgcgc cgtctcaatg agccgcttac cgttttccag tatctgatcc acgtcgatca
    39901 cccagctgtc cggggcgatt ttgccctgct gccacaaact tagtgcggcg gaaatcagcg
    39961 ccgggttctg gcgtttcaat gcttctacaa acatcttgtt gcctcgtttc tgttccttga
    40021 attaaatatt cattttttta aatatttagg ttgtgcgacg tggatttgtc gggagcttca
    40081 aacctcttcg ttagcagcca tccataatcc ataaagattg gccagaaaat agccttcttc
    40141 acacgggtta acttccagag cgaattcctt caacaacacc tgatgcaatt gcacaacggc
    40201 ttgccagtgg ctggattgcg ccagttccgc cagcaactcg ttatccagcg gctctatttc
    40261 ttcaccacgg cgactgcgca tcagtgcact cgccatatgt gtcatcgcca tcgttccttg
    40321 ctcactgcgc accggcagat ggcactcttt ttccagtacg ttgacgacct gcatcatgcc
    40381 tttgcagacg tccttatcaa taacgcctgc ctcgcaaagc aggttgagtc tggtttccat
    40441 atcagatgcc tttcgtttaa taactgggtt cctgttcccc actcaccacc cgttgttgat
    40501 gggcaagcag ggcgtgttta tctacaacgg cacgcagcaa ttgttgcatt gggtaatcat
    40561 cgactcgcgt caggaccacg gcttcggtgg ccagtaaaaa tcgcggatcg tctgttagcg
    40621 gcgtcgcctc taatcccagc atggtcagtt cgttttccgc caccacgttc cagatcaccg
    40681 catcgacatc gcctttaaca atgcgttgta aactctcgtg ataagagaga tcgactcgtt
    40741 ccacatcact atcgccaaaa aagacatcgg tcatgatttt ctgatccgcc gaacgactat
    40801 cgagacctac tcgcttaaca tttccagact cacctttgcg gcatatcagt tgatgttcac
    40861 caacatacgt atgcgggcca agctcgaggg cgatgcacaa attgttttgg ctgagataac
    40921 tttccgccgc caggcgggaa actactgcca tgtcatagac accgttaagt aaacactcca
    40981 cgcgaatatc cgcgccgcgc atatgcgcat agtaaaaagg aatgccatca aactgggctt
    41041 tcaatccgct cgctaaacct tcgtacaagc gggtataggg caaaggcatc gcacatacca
    41101 cgttgttgat atccacatga ctcagcaatg ctttgttatc catctcgaca agataactgc
    41161 cattgcgccc acggcgttca atccgtatcg ctccgcttga ttccagcgtt ttcaacgcgg
    41221 cctgcgtcag gccaaccgat gaacggcatt cagttgccag ctcatctatg gttttcagtc
    41281 gattaccgca tttttcaccc aacaaataac gggccagtgt cgtgatgacg acgccttctt
    41341 ttttgataaa cgttcgacgc ataatatatt ttcaataaaa tgaatattta tatcttcatt
    41401 aaaatgaaga taagtggcga cgacgagatg tggaaacggc gaggcgcttc acattttttc
    41461 gggttgttgc gaaaagagga taaaaaaacc gggtttcccc ggtttcagag tgatgacgaa
    41521 gtgatttatt cgacaatgcg agcaaagggt aattcccact ggcgcttgcc tgctcccctc
    41581 accctaaccc tctccccaaa ggggcgaggg gaccgtccga gcgttttgat attacggctt
    41641 cgccacaaaa ccaatcgctt cgtacaccgc tttcagcgta cgggaagcgt gtacactggc
    41701 tttttctgcg ccgtctttca tcacctgttg caggaaggct tcatcgttgc ggaaacggtg
    41761 atagcgttcc tgcaattcag tcagcatacc ggaaacggca tcagccactt cacctttcag
    41821 atgaccatac atcttgcctt cgaactgttt ttccagttct gggatgctct ggcccgttac
    41881 cgcagaaagg atatccagca ggttggaaac gcccgctttg ttctgcacat cgtagcgaac
    41941 taccggcggc tcgtcggagt cagtgaccgc gcgtttgatt ttcttcacta ccgatttcgg
    42001 atcttccagc aggccgataa cgttattgcg gttatcgtca gacttggaca tcttcttggt
    42061 cggctccagc agcgacatta cgcgcgcgcc agatttcgga ataaacggct ccggcacctt
    42121 aaagatctcg ccatacagcg cgttgaaacg ctgggcgata tcgcgactca gttccaggtg
    42181 ctgtttctgg tcttcaccca ccggtaccag attagtttga tacagcagga tgtccgcagc
    42241 catcagcacc ggatagtcaa acagaccagc gttgatgttc tcggcataac gcgcagattt
    42301 atctttaaac tgcgtcatgc gactcagttc gccgaagtag gtatagcagt tcagtgccca
    42361 gcctaactgc gcatgttctg gtacgtggga ctgaacaaaa atggtgcttt tctcaggatc
    42421 gataccacaa gccagataca aggccagcgt atccaacgtc gctttacgca gcttctgtgc
    42481 atcctggcgc acggtgatcg cgtgttggtc aacgatacag taaatgcaat ggtagtcatc
    42541 ctgcatgttt acccactgac gcagcgcacc catgtagtta ccaatggtca attcacctga
    42601 gggctgtgcg ccactaaaaa cgatgggctt agtcattttt cgattcctga ttttcgctat
    42661 gcggaagccc taatgcgggc agaaggtcat ttatagactg ataaattaca tcaggctggc
    42721 tgagatcgat agcctcgccg tagttatatc cgtaggttaa gccaactgat gggcaacctg
    42781 ccgcttttgc cgcctgaata tcattgcgtg agtcgccgac aaacagcatc tgttgtgggg
    42841 caattcccat ccgctcagcc accagtaaca gcgggtccgg atgcggtttt ttgttttgca
    42901 catcatcgcc gccgataacc acgctaaagt atttggcaat atctaaggct tcgagcagcg
    42961 gcgcgacgaa cggcgtcggt ttgttggtga ccaggcctag cggcaggcct ttagcctgca
    43021 acgcgcccaa cgtatcggca acgtgcggga acaaaaacgt cccctcttcg gcaacctcgc
    43081 catagtagcg atcgaacagt ttacgcagaa tacgtacctg ttcttctgcc ggaatgtcgt
    43141 catcaacggg cggtttcccc attgttttac gcagagtcgc acgttcctga cgcgcccagg
    43201 tcaatgcgcg ctccatcaga acatctgcgc cgttaccaat ccaggtaata acgcgttctt
    43261 cacctgcgac gggcaactcc agcgcataca gcgccatatc taccgcagca gcaagaccag
    43321 gagcactgtc gaccagcgta ccatcaagat caaaagcgac gccgcgaata tcttcaaact
    43381 tattcatgac ttacctttgc cagttcactg cgcatttcat caatgacttt tttatagtct
    43441 ggctggtcga agattgccga accggcgacg aacatatccg cgcccgccgc agcgatttcg
    43501 ccaatgttgt tcaccttcac gccaccgtcc acttccagac gaatgtcaaa gccagactcg
    43561 tcgatacggc gacgtacttc gcgcagttta tccagtgttt gaggaatgaa agactgaccg
    43621 ccgaaaccag ggttgacgga catcagcagg atcacatcca gcttatccat cacgtaatcc
    43681 agatagctca gaggtgtcgc cgggttaaat accagacccg ctttacagcc attttcttta
    43741 atcagttgca gcgtgcggtc aacatgctcg gaggcttctg gatgaaaggt aatgatgctg
    43801 gcaccagcgg cagcgaaatc aggcacaatg cgatcgacgg gtttcaccat cagatgtacg
    43861 tcgatagggg cggtaatgcc gtagttacgc aaggatttca gcaccattgg cccaatcgtc
    43921 agattgggaa catagtggtt atccatgacg tcaaaatgca cgacatcagc gccagctgcc
    43981 agggcttttg cggtatcttc acccaggcgg gcaaaatcag ccgacagaat tgagggggca
    44041 atcaaatact gtttcatccg cttctccttg agaattattt tttcgcgggt gaaacgactc
    44101 ctggtttgta caaagccagc agttcgtcca cctttttacg tgtgccgccg ttgctgctta
    44161 tactgcgtcg aactttgacg acatgcaatt ttgcgcgctg ataccactca cgcgttaaca
    44221 tcgtatcgtg attggagatc agcactggaa tatggcgctc aaccagacct tcggcgatct
    44281 ccgccagatg cgcttgttgt tcaagcgtaa aactgtttgt gtgatacgcc gtaaagttgg
    44341 cggtcgcaga cagcggtgca taaggcggat cgcaatagac gacggatgca tcatctgcgc
    44401 gctccatgct atcggcgtaa gactcacaat agaaaaaggc attctgcgct ttttcagcga
    44461 agtgatacaa ctctgcttcc gggaaatagg gttttttgta gcggccaaac ggcacgttaa
    44521 actcaccgcg cagattgtaa cgacacaggc cgttgtaacc gtagcggttc aaatataaaa
    44581 acagtaccgc ccgacggaac ggatcctggc ttttgttgaa ctcttcgcgg aactgatagt
    44641 aaacctcggc gcaatttgtt tcgggaacaa acagctcgcg tgcggcctgt acgtactcat
    44701 cagtacgcat cttcacaatg ttatagagac tgatcaggtc gctattgata tcggcaagga
    44761 tataacgaga aaagtcggtg ttgagaaaca ccgacccggc acctacaaaa ggctcaacca
    44821 gacattcgcc cttgggcaaa tgccgtttaa tatcatcaag caggggatac ttgccccctg
    44881 cccacttcaa aaaagcgcga tttttcttca tgccgactaa ctaattacac cttctccggc
    44941 tgtggagaaa gctccgacag catccagcgc ttcaggcagc tcccgcaacg ataaaccatt
    45001 gcgggagata accttgatta cttcagatcg gcctgtacct gacgcagcgg tttcgcccac
    45061 gggtttttgg cctgaacatc tgctggcaat gtagataccg cttttttcgc ctcttctttc
    45121 gaagcgtaca cgccagaaac caggacatac cacggctgac cattacgcgt cgtttcatag
    45181 acaacgtagt ttttcagatt ctctttcttc gcccaaccgt tcaggttgtc gtagttagag
    45241 gaactgctca gctgcagagt gtaatggctg gacggtgccg atttcaacga accaacatta
    45301 cctgcggtct tccctccagc ggctggtgtt gccgtggttt gcgccgggga tgccgtctgt
    45361 actggagccg tagtcgccgt ctcttttggt gctggcgtag aagtcgcagc cggtgccttc
    45421 gtgctcgcca caggggcagc tggttcagta cgcttcggcg tctgtgctac cggcttcggc
    45481 tccgttttca cggttgcttg cggttttttc ggttcaatca ccgcctgctg acgaactggg
    45541 cgcgtagtgg acggacgttc agcggtttgc gttttcgccg tgtcacgcga tgcattgcca
    45601 ttgcgaacag gcgcaaccgt tgcgggttca gtcggcaacg tggaattgac cgccacattg
    45661 ttcagctgtt gctgattttg tggctgggtc agcgcattgt tcaggtcacc ctgcacttca
    45721 acacgttgtt gaccatccgt tgccaccggg gtttgccctt gagtcggcgt agaagagatc
    45781 ggtggcagag aaacatcctg ctgagtattt tccgcagacg tggttcccgg cgctggctgc
    45841 acgccattcg cctgatcggt cgcattgcca gcaagatcaa tactcttctc gccagacgcg
    45901 gtttgatcgc tggaagtggt cgagggggct tttagcgcag aaccgatacc gatgatcaac
    45961 agcagtagaa ccagaatgcc gacgcccatc atcatatact gacgagaagc gggtttactg
    46021 gctgcttttt tgcgcttacg cggacgacgc tctacgcgct cttcatccac ggtttcatct
    46081 tcggattcgt caatttcttc ttcgatttcc ggttcctcat tgcgctcttt ttgcgcacga
    46141 gtcggacggc gatcgtcagt gtcatcaagt tcaatatcat caaaattgat ctgcggttcg
    46201 ccacgttcag tacgctcaga acgttcagaa gattgacgag aacgaccagt acgacgatcg
    46261 ctgggatcgg gtttcagctc gtcttctggt ttgaattcat ccatttaaca ccccactaaa
    46321 aggttaatgc ttaccacgtt gcaattaacc tgaagctaat agaccgcttg ataagcggcc
    46381 tgacctttct tgttgttacg ctgattgaca atcggcaatg gcgttaagaa caagctcgtg
    46441 cgaaacgccg ctgcgaactt cactcttacc aattgccaac ggaagaatta agcgcatctc
    46501 tcccgcaagg actttcttgt cacgcagcat atgcggtaaa tacgcctgcg cggacatttc
    46561 gcgcggccca ttgaccggta acccagcccg cgtgagcagg gttataatac gctgcgtttc
    46621 ggcagaacta aactgcccga gacgttccga cgtccgcgcc gccatcacca tacccgcagc
    46681 gaccgcttca ccatgtaacc aattgccata ccccatttca gcttcaatgg catgaccaaa
    46741 ggtgtgtccc agattcagta aagcacgtaa cccggtttcg cgctcgtcgg cggcgacaac
    46801 ttctgccttc agttcacaac aacggcgaat acagtacgcc attgccggac cgtccagacg
    46861 caacaacgca tccagattct cttccagcca gttaaaaaac gcaccgtcaa gaataatgcc
    46921 gtatttgatg acttctgcca gccccgacgc taactcacgc gggggaagcg ttttcagaca
    46981 gtcgagatcc accaccactg aagcaggctg gtagaacgcg ccaatcatgt ttttaccgag
    47041 gggatggttg accgcagttt tgccgccaac ggaggaatcg acctgcgaca gtaacgtcgt
    47101 cgggacttga atgaaacgaa caccgcgctg ataactcgcc gccgcgaagc cggtcagatc
    47161 gcccactacg ccgccgccaa gcgccaccag cgtagtatcg cgaccatgcg gcttttgtaa
    47221 caacgccgta aagacggtat cgagtacagc taggctttta tactgctcgc cgtcagggag
    47281 gataacgcta tcgacgttaa cacccgcctg ttcaagtacg ccgcggacct tatcgagata
    47341 cagaggagcc agggtttcgc tggtgaccaa catgacctgc tcgcccgatt tcagcggtaa
    47401 gaatgaagct ggttcattaa acaaaccaga tgcgatggta attgggtaac tacgttcccc
    47461 gagagtaacg acaatcctct ccataacgcg acatccacct taattactgt acccgcagac
    47521 gagtgtatat aaagccagaa ttagttgctt tccagcatgt gaataatctg gtttgcaacc
    47581 actttagcgc tttgatcatc agtacgaatg gtcacgtcgg caatctcttc atacagcgga
    47641 ttgcgttcat tggccaacgc ttccagaact tcacgcggcg gtgtttcaac gtgcagcaac
    47701 gggcgttttt tatcacgctg cgtgcgtgca agttgctttt cgatggtcgt ttcaagataa
    47761 acgacaacgc cacgagcgga aagacggtta cgcgtttcac gggatttcac agagccgccg
    47821 ccagtagcca gcacaatacc ctgtttctcg gtcaactcat tgatgacctt ttcttcgcga
    47881 tcgcggaagc cttcttcgcc ttctaaatcg aaaacccagc ccacatcagc tccggttcgt
    47941 ttctcaatct cttgatcgga atcgtaaaat tccatattga gtttttgagc taactggcgc
    48001 ccaatagtgc tttttccggc acccataggc ccaaccagaa agatattgcg tttctctgcc
    48061 attttttcgg tactactaag actattcgtt aatgataaac ccgcttcgct cagagagcgc
    48121 cgcaggacat gaactgaaac ctcataagat attgcgagag tcagactgaa aattatctca
    48181 atactccagc gggtttggca actgaataaa tcacccatcc ctctgcatat ctggtcgctg
    48241 caagcgcact gccttgctac caccgctctg gcgataaatc accgggtaag attagcgtaa
    48301 aaaagacagc aaaatgccgc ctgaatgata aatcatcatc atggaacgca ggcagcgaag
    48361 agcaccaact ctcaaatcgg tactccttgt atgctaaata cctgcgcgcg tcaaatagat
    48421 gaaacacgtt cagcataaaa acattaccgc ttttacggct gtttactcac tggaaaccag
    48481 tcgtggcgtg ataaacacca ctaactcgcg tcgttcatct tcttttccgt catgacgaaa
    48541 taattgcccg aaccagggaa tgtcgccaag caacggtacg ctatcctgac ccgatttatt
    48601 tttacgggta aaaatgccgc ccagcgccaa cgtttctccg ctttttacct cgacctgcgt
    48661 ttcgatctcc tgcttatcaa tcgccagcac ttcgccatcg gcctgctgta gcacctgccc
    48721 cggaacgttc tggctgatgt gtaatttcag ccggatgcga cctttttgta acaccgtggg
    48781 cgtgacctcc atccccagga cggcctcttt aaattccacc gacgtcgcgc cactttcccc
    48841 gctggaagcc tgatatggaa tttcgctccc ctgtttaatg ctggcaggct gaagatgtga
    48901 ggccagcaga cgcggactgg cgataatatc cagctgctgt ttttgttcga gcgcggaaag
    48961 ctcaagatcc agcaagcgtc cgttgatgcg cccaatgtta aaaccgacat gcgttgtcgc
    49021 cgtcgccacg gagaggtcgc taccaagcgt ggtgacttgc ccaacgccac cagcgtgttg
    49081 cgcatcggcc agcgtccatt tcacgcctaa ctcacgcaaa cttttttcat taatggtgac
    49141 aatatgcgcc gacagctcaa cctgcccgac cggcagatcc atttgcgcta cccactgttc
    49201 aagcgcgctt aacgccgttt tgttatctcg tagcaaaagg cgattggtgc gtttatcgac
    49261 ggtcatactc cctttggcac tcagtagctt ctcccccgct ttcgccagtt ctcccgcgtc
    49321 ggcgtattgc agggttatac tgcgattttc cagcggcaga tttgcctgcg cccgcgcctg
    49381 ctccgcctcc tggcgggcga tattgtcatt ctgccaggca atggaatgca ctgagagaat
    49441 gttgccttcc tgccgcgtta tcagtccggc gcttttcact acagtttgta gtgcctgctt
    49501 ccagggaaca tctgttagat gtaacgacac cgtaccgctg acgtctggcg acacgaccag
    49561 gttcaacttc tcctgttcag ccagcgcctg caacacctga gctaccggaa cgtcatccac
    49621 catcagcgtc actttttgcg gctttgccgc ctgtacgccg ggtatcagca tcaacagtag
    49681 tgcggctatc cattgcttca tttgtatctc cttgccgttg ccacaaccat tgtggcggtt
    49741 cgcagtttgt cccggtaccc agcgttagta cgtctggcgt cagctgtaaa attgtccagc
    49801 cgttttccag cacatcgttt tgctgcaccc gtcgccattt cttttgcccg tcttttatta
    49861 caccgatgat gcgctcgcct cgccctacca tcccctgata gcgccactgg ctaagttcgc
    49921 taatccggca tagatcttcc ggcgatttaa aagggtcacg cataccggtt aaaaggcaca
    49981 atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg gcatctccag ttgtagcgtg
    50041 aacaaaagat catcaccttc cacgcttaac gaaaaacggc tcacgctgac gttgcgctct
    50101 gccagccgtg taaatgccga cggcactgct tcccacagcg ttttcaacgc caactcgcct
    50161 ccctgcgcgg atggatgcca ggaaaccagt tgcgcgccgg ataactgaaa atccagtggc
    50221 gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt ctaccaggcg atacagtgcg
    50281 gcccactgct ggtgatgact tgcccgtagt cgaattaatg cgtcgcgctc ttcatggtgt
    50341 gtcgatgata gaaaaatgag cgtaactaac atcagcaacc agaatgccca gcaaaactgg
    50401 cggaggcggg gtgatgtggc gaaccaccag tcaaagaaca tgttcatcgc taaccttcct
    50461 tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc tgctgcgtgg ctccccgctg
    50521 attgagatga aaagacgcat cctggcggag tgacgtttct agtgcgttta acgcggtaat
    50581 gcttgttgtc agccccttga tctccagcgt tccctgctgc cagcttatcg ttgtcagcca
    50641 ggcgtgctcg ggtaaaagcg ccgccagtgc ttccagcgca gattgccagt cgcgggtaaa
    50701 ttgtcgctgg cgctgccgtt gcgaacgctg ttcgcgtaac tgctgttgct ccagcaaacg
    50761 tggcttcgtt atctgtaagc tgcgggcgag ttgttgttcc gcctgaagca aaactgcgtc
    50821 tatgcgcgct tcggcgctgc ctgtcagacg cagtattagc gttatcccga cggccagcag
    50881 cagaggcgca acgaacatca gcaaccagaa acgcagaaaa gcggtccggc gttgctgtcg
    50941 ccagggcaaa aaattaattg gcgggttcat cagtacgcct tcccaagcgc cagccccagc
    51001 gcgatggcaa agtctccacc aggcggcggt agcggcggct gacgaacaga aacggcctcc
    51061 caggggtcaa atccgccttc gccacatatc gcgacgctgt cgggatcaac ggataacgct
    51121 gccgccagct ccttcgcgct agtcatcccc accgccagtt tgcgccccca gctatagcgc
    51181 gtcgcccaca gccactgttc gttatcacgc caggccagac attgctgatg agaaggtaaa
    51241 aaaggcagga atcgctgtaa tgcactggca tccggggtga tcgcactaac atgaacacgc
    51301 aacctttctg ccagcgtaag cagcgttgcc agctctttgc tttgcgcggc agtcacgtta
    51361 taagcggggc tgagtgagtc ttcgctgtaa tcgaagcgca gggagtccgg atccatatcc
    51421 agctcgcggg ccatcgtccc tgacagccag gccgtttgct cccgctcacc aagggacatc
    51481 gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg gaaacgccaa cataatgtga
    51541 tgacgctgcg gcagttcgcg actccacggt aacaacgttt tagccagctg ctgcgcatca
    51601 acaatccgcc catctttgat aatgtcgtgc gccagcggca accgccacca gcgttgcaaa
    51661 aagcattctt ttgcgccccg cacgatcgca accgctgccg cttcttgctg ttgtaaatgc
    51721 aaaccaattt gccagatctt aaatgtcatt gtgatgatct ccttatcact cgtcactctg
    51781 acgggtatat caatgcgtct ggcttgcctt tatactaccg cgcgtttgtt tataaactgc
    51841 ccaaatgaaa ctaaatggga aatttccagt gaagttcgta aagtattttt tgatccttgc
    51901 agtctgttgc attctgctgg gagcaggctc gatttatggc ctataccgct acatcgagcc
    51961 acaactgccg gatgtggtga cattaaaaga tgttcgcctg caaattccga tgcagattta
    52021 cagcgccgat ggcgagctga ttgctcaata cggtgagaaa cgtcgtattc cggttacgtt
    52081 ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg acggaagaca gccgcttcta
    52141 cgagcatcac ggcgttgacc cggtggggat cttccgtgcg gcaagtgtgg cgctgttctc
    52201 cggccacgcg tcacaagggg cgagtaccat tacccagcag ctggcgagaa atttcttcct
    52261 cagtccagaa cgcacgctga tgcgtaagat taaggaagtc ttcctcgcga ttcgcattga
    52321 acagttgctg acgaaagacg aaatcctcga gctttatctg aacaagattt accttggtta
    52381 ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc ggtaaaacgg tcgaccaact
    52441 gacgctgaac gaaatggcgg tgatagccgg gttgccgaaa gcgccttcca cctttaatcc
    52501 gctctactca atggatcgtg ccgtcgcgcg gcgtaacgtc gtactgtcgc ggatgctgga
    52561 tgaagggtat atcacccagc aacagttcga tcagacacgc actgaggcga ttaacgctaa
    52621 ttatcacgcg ccggagattg ctttctctgc gccgtacctg agcgaaatgg tgcgccagga
    52681 gatgtataac cgttatggcg aaagtgccta tgaagacggt tatcgcattt acaccaccat
    52741 cacccgcaaa gtgcagcagg ccgcgcagca ggcggtacgt aataacgtgc tggactacga
    52801 catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg aaagtgggcg agtcggcgtg
    52861 ggataacaac aagattaccg atacgctgaa ggcgctgccg acctatggtc cgctgctgcc
    52921 tgccgcagtc accagcgcca atcctcaggg agcgacggcg atgctggcgg acgggtcgac
    52981 cgtcgcattg agtatggaag gcgttcgctg ggcgcgtcct taccgttcgg atactcagca
    53041 gggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg ggtcagcaaa tctgggttcg
    53101 tcaggttggc gatgcatggt ggctggcaca agtgccggaa gtgaactcgg cgctggtgtc
    53161 gatcaatccg caaaatggtg ccgttatggc gctggtcggt ggctttgatt tcaatcagag
    53221 caagtttaac cgcgccaccc aggcactgcg tcaggtgggt tccaacatca aaccgttcct
    53281 ctacaccgcg gcgatggata aaggtctgac gctggcaagt atgttgaacg atgtgccaat
    53341 ttctcgctgg gatgcaggtg ccggttctga ctggcagccg aagaactcac caccgcagta
    53401 tgctggtcca attcgcttac gtcaggggct gggtcagtcg aaaaacgtgg tgatggtacg
    53461 tgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat ctgcaacgct tcggcttccc
    53521 ggcgcaaaac attgtccaca ccgaatcgct ggcgctgggt tcagcgtcct tcaccccaat
    53581 gcaggtggcg cgcggctacg cggtcatggc gaacggcggc ttcctagtgg acccgtggtt
    53641 tatcagcaaa attgaaaacg atcagggcgg cgtgattttc gaagcgaaac cgaaagtagc
    53701 ctgcccggaa tgcgatattc cagtgattta cggtgatacg cagaaatcga acgtgctgga
    53761 aaataacgat gttgaagatg tcgctatctc ccgcgagcag cagaatgttt ctgtaccaat
    53821 gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag actggcgcgc aggagtacgc
    53881 accgcacgtc atcaacactc cgctggcatt cctgattaag agtgctttga acaccaatat
    53941 ctttggtgaa ccaggctggc agggtaccgg ctggcgtgca ggtcgtgatt tgcagcgtcg
    54001 cgatatcggc gggaaaaccg gtaccactaa cagttcgaaa gatgcgtggt tctcgggtta
    54061 cggtccgggc gttgtgacct cggtctggat tggctttgat gatcaccgtc gtaatctcgg
    54121 tcatacaacg gcttccggag cgattaaaga tcagatctca ggttacgaag gcggtgccaa
    54181 gagtgcccag cctgcatggg acgcttatat gaaagccgtt cttgaaggtg tgccggagca
    54241 gccgctgacg ccgcctccgg gtattgtgac ggtgaatatc gatcgcagca ccgggcagtt
    54301 agctaatggt ggcaacagcc gcgaagagta tttcatcgaa ggtacgcagc cgacacaaca
    54361 ggcggtgcac gaggtgggaa cgaccattat cgataatggt gaggcacagg aattgttctg
    54421 attaaaaagg cgcttcggcg ccttttcagt tggctgacaa agtgcatttg tttatgccgg
    54481 atgcgggtga acgcgttatc cggctaacaa aatcgtgaaa aatcaatata ttgcaggaac
    54541 cccataagcc tgataagcgt tgtgcatcag gcaaacttca cgcatttaca ctcgcccctg
    54601 ccctttcaac cattcgcgca cgaggaacag cgcgctgaca ttacgcgctt cgttgaagtc
    54661 agggtcttcc agcaaatcca tcatatgcgc cagcggccag cgcacctgtg gtagcggctc
    54721 tggctcatcg ccttccagcg attccgggta gagatcttgc gcgaccacga tattcatttt
    54781 actggaaaag taagacggtg ccatgctgag cttcttcaaa aaagtcagat cgttcgcacc
    54841 aaatccaacc tcttctttta gctcgcggtt agcggcttcg tagacgcttt cacccggatc
    54901 aattaatcct ttcgaaaaac ctaattcgta ggattcagtt cccactgcgt attcgcggat
    54961 caggatcagg tgatcgtcca caatcggcac aatcatcact gcttcccggt tggttggacg
    55021 cattcgttca taaacacgcc gcacgccatt gctgaactcc agatccacac tctcgacggt
    55081 aaacagtcgg gaacgggcta cagtttcaac attcagaatg gtgggttttt gtaatgattt
    55141 gttcatcgtg ggatctatgc tgtgaaatca gcggttattg tgcgatatcg gacacgcttt
    55201 cggcaatgtg aattgcatgt tatttacatt tatgtaactt aataaataat cgtcctcaaa
    55261 tcaaattaaa agtcaatagg ttgaaataac tccaggaatt tgctgatatt ccgccttcag
    55321 agggtttgct atgatcagcg gttactgtga tgtgcttaat gatgctcaag ttaaactcca
    55381 cgcttgccga tagccaaccg cagaatcatg tattgtgtcc ggtgcgactg accacgcctg
    55441 acagactaag taagatgggg aaagcatgag caccattgtg atttttttag ctgctttgct
    55501 ggcctgctca ctacttgcgg gatggctgat aaaagtgcga tccagacagc gtcagctgcc
    55561 ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc acacctgaag aacgtagcgc
    55621 cgttgaaaat tatcttgaga gcctgacgca ggtattacag gtgcctggcc caacgggagc
    55681 tagcgcggca ccgatctctc tggtgctgaa tgccgaaagc aacaacgtca tgatgctgac
    55741 acacgctatc acacgttacg gcatctctac cgacgatccg aataaatggc gttactacct
    55801 cgattcggta gaagtccacc tgcccccctt ctgggaacag tacatcaacg atgagaatac
    55861 cgttgaactg attcataccg attcgctgcc gttggttatt tcactcaacg gtcatacgct
    55921 gcaggagtac atgcaggaaa cgcgcggcta tgccttgcaa cctgttccgt ccacgcaggc
    55981 gtcgattcgc ggggaagaga gtgagcaaat cgagctactc aatattcgca aagaaacgca
    56041 tgaagaatat gcgctgagtc gtccgcgcgg gctgcgtgaa gcgttgctga tcgtcgcctc
    56101 cttcctgatg ttctttttct gcctgattac cccggatgta tttgttccgt ggctggcagg
    56161 cggcgcatta ctgctgctgg gcgcaggtct gtgggggcta ttcgcgcccc cggcaaaatc
    56221 ctccctgcga gaaattcatt gtctgcgcgg tacaccccgt cgttggggat tgtttggcga
    56281 aaacgatcag gaacagatca acaatatttc actcggtatt atcgacctgg tctatcccgc
    56341 acactggcag ccatacattg ctcaggatct cggtcaacaa accgatattg atatctatct
    56401 cgaccgccac gtagtgcgtc aggggcgata tctttcgctg catgatgaag taaaaaactt
    56461 tccgttacag cactggctgc gcagtacgat tatcgctgcg ggctcgctgc tggtgctgtt
    56521 tatgctgtta ttctggatcc cgctggatat gccgctgaaa ttcactctct catggatgaa
    56581 aggcgcgcag accattgaag ccaccagcgt aaaacaactg gctgacgctg gcgtgcgggt
    56641 aggcgataca ttgcgtatta gcggtacggg aatgtgtaat attcgcactt ccggaacctg
    56701 gagcgcgaaa accaattcac cttttttacc atttgactgc tcgcagatca tctggaatga
    56761 cgcccgctca ttaccattac cagaatctga actggtcaac aaagcgacgg cattgactga
    56821 agcagttaat cgccagctgc accctaaacc ggaagatgaa tctcgcgtca gtgcctcatt
    56881 acgttcagcg attcaaaaat ccggcatggt attgcttgat gattttggcg acattgtact
    56941 gaagacagcg gatttatgtt ctgccaaaga tgactgtgtg cgactgaaaa atgcgctggt
    57001 caatctcggc aacagtaaag actgggacgc gctggtaaaa cgcgccaacg ctgggaagct
    57061 cgatggcgtg aatgtgttat tacgcccggt gagtgcggaa tcgctggata acctggtggc
    57121 aacctccacc gcgccgttta tcacgcatga aacggcgcga gcggcacaat cactaaacag
    57181 tccggccccc ggcggattcc tgattgtcag cgacgaaggc agcgattttg ttgatcagcc
    57241 ctggccttcg gcatcacttt acgactaccc gccgcaagaa cagtggaacg ctttccagaa
    57301 actggcacaa atgctgatgc atacgccgtt taacgccgaa ggtatcgtca caaaaatctt
    57361 cactgacgcc aatggtacgc agcatattgg ccttcatccg atcccggatc gttccggcct
    57421 gtggcgctat ctcagcacca cattgctgct actgacgatg ctgggtagcg ccatttacaa
    57481 tggcgtacag gcctggcgtc gttaccagcg tcatcgcact cgcatgatgg agattcaggc
    57541 ctattatgaa agctgcctga acccgcaact gatcacccct tcagaaagcc ttatcgaata
    57601 acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact ctggagattt
    57661 ccctcatgca tatcaacatt gcctggcagg acgtagatac cgttctgctg gatatggacg
    57721 gcacgttgct cgacctcgcc ttcgataact atttctggca aaagctggtg cctgaaacat
    57781 ggggcgcgaa aaacggggtt acgccacagg aagcgatgga atatatgcgc cagcaatatc
    57841 acgacgtaca gcatacgcta aactggtact gtcttgatta ctggagtgag caactgggtc
    57901 tggatatctg tgcgatgacc accgagatgg gaccgcgcgc cgtactgcgt gaagatacca
    57961 ttccgtttct tgaggcactg aaagccagcg gtaagcagcg aattttgctc accaatgctc
    58021 atccgcacaa cctggcggta aaacttgagc atacgggtct ggacgcacac cttgatttat
    58081 tactttccac ccacacattt ggttatccga aagaggatca gcggttatgg catgcggtgg
    58141 ccgaagctac gggtctgaaa gctgaaagaa cgctgtttat tgatgacagc gaagcgattc
    58201 tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg cgtgactaat cctgattccg
    58261 ggattgccga gaaacagtat caacgccatc cgtcactgaa tgactaccgc cgcctgatcc
    58321 cctcgctaat gtgaaggaga cgccatgaaa gagaaacctg ctgttgaggt tcgactggat
    58381 aaatggctat gggctgcccg tttttataaa acccgcgcgc tggcccgtga aatgattaaa
    58441 ggcggtaagg tgcattacaa cgggcagcgc agcaagccga gcaaaatcgt cgagctgaat
    58501 gccacgctca ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa ggcgattact
    58561 gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg aagagactgc ggaaagtgta
    58621 gagaaacgcg aaaaaatggc gatggcacgt aaacttaatg ccttaaccat gccgcacccg
    58681 gaccgacgcc cggacaaaaa agagcgccgc gacctgttac gatttaaaca cggcgacagt
    58741 gaataactgt cacctgcaag agagatgatt atgccgcaac atgaccaatt acatcgctat
    58801 ctgtttgaaa attttgccgt tcgcggcgaa ctggtaaccg tttcggaaac cctgcaacag
    58861 atccttgaga accacgatta tccgcagccc gttaaaaacg tgctggcaga actgctggtt
    58921 gcgaccagcc tgttaaccgc tacgctgaag tttgatggtg atatcaccgt acagctgcag
    58981 ggcgatggtc cgatgaatct ggcggttatt aacggtaaca ataaccagca aatgcgcggt
    59041 gtggcgcgcg tgcagggcga aattccagaa aatgccgacc tgaaaacgct ggtcggcaat
    59101 ggttacgtgg tgatcaccat taccccgagc gaaggcgaac gctatcaggg cgtagttggt
    59161 ctggaaggtg ataccctggc ggcctgccta gaagattact ttatgcgttc tgaacagctg
    59221 ccgacgcgcc tgtttattcg caccggcgac gtagacggca aaccggctgc aggcggtatg
    59281 ttgttgcagg taatgcctgc gcaaaatacc cagcaggacg actttgacca cctggcgacg
    59341 ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac cggcaaacga agtgttgtgg
    59401 cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc aggatgtgga gttcaaatgc
    59461 acctgctcgc gtgaacgttg cgccgatgcg ctgaaaacgc tgcctgatga agaagttgat
    59521 agcatcctgg cggaagatgg cgaaattgac atgcattgtg attactgcgg taaccactat
    59581 ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg cgtctccggc agatccgcaa
    59641 gttcattaat gagtatgtcc ggcagagagg gctctgccgg attttacgac ttaatggcct
    59701 gatgtgctgc gcttaccagc gcctatgaaa taaatcgcaa attattttgg tcagatacag
    59761 cgtcgtatcc gataaaagcg ttgtcactta cttaagttta tttccagcgt ttcaccatct
    59821 accgtcagat cgcgccacgc tttcggcctc gtccccagtt ttccatcctt cgcagctgtg
    59881 agtaattttt ctttcgttat ttcgttcgga agtaaactca tatcaagcgc gtcccattct
    59941 tttctgtctg agtcaaagcc atagacaatg actctgtcat cattaaaagc aaacaggatc
    60001 cgctccgcct gaccatcact attcagatct tgctcgacca gtacgcaggc gtctttttca
    60061 atacaggtca tcacgttata gcgatcctgg attaaggccg accagaatgt cgcatccggt
    60121 ttaacagaac caggggcaat taacacattt tcggctaata ctttttccga tacctgttcc
    60181 tgaagatgtt gctctccatc taacgccatc agcagatccc gcgcgcgttt cgggtctttc
    60241 ataaacccgg catcgctttt cagcgactca agcgccgcac gtccatagcg accgctctgt
    60301 tcgagcatgt agatagttac ctggtctggc gtgtttttgc cgctctgata acgcgccata
    60361 tgactgttca cgctaatacg catactgtcc agcaccggcg aattaagaag caccagtatc
    60421 accaacacca ataacgaaac cgcaaggttc actttgccct gaagaacaag gggattttgc
    60481 ccattacgcc agacgatact gacaaaatac cccagcgacc acaccagtaa caccagctcc
    60541 gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact gagcgacccg cagccataac
    60601 ccccaggtgg cgacgaacac atacagcgga gcaaccagca aagcggtttt aatcaggcaa
    60661 cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat cgcggacaat agccattaag
    60721 atcaattgca aaaaggccag cgttaacagc aacccggcgg cggagatgtg gcgagaaatc
    60781 gcgctcaggc ccgtaaacgg cagggtgatg ataaacatca gggttagcaa tgatactaac
    60841 ggcagcaacc ccgtggcgat tagcgtgaac aacttttgaa tagagtcgat taaacgtgac
    60901 tgtgtacgcg cgaggatcac cgccagtgcg gtaaccagac caaatgtaag atacataaac
    60961 cagtcggttg caaaaaagag cgtattgaaa aacgtgatgc caacaagttt aaacaactca
    61021 ccccacagta aaagcaccag ccacgtcagg ccattggaga gaaaaatcac cagtaatata
    61081 agtacgttat gccatactga ctggtaaaag tagctatagc ggctactgcc attgcggata
    61141 cgaaggcttt gttgtatcca cggtagcaac aacattgcca tcaacagcag atagcagcca
    61201 aaatcccaaa tcgccttttc agctctccag gggttcatgc catcagtttg ccacttcagc
    61261 caaccgctca tccctgtcgt ggcaataaac accagcgcca gccatcccca aaggcgcttc
    61321 tgtttaaaag agatcacgga gaagagtaaa acggatgaaa acgcgacggt cgcaggcacg
    61381 ccataaacaa tccaactgtg attttttccg gcaagccagg cgataagcag gtagcaaacc
    61441 agcccctgaa gtaatccggt agcaatcatc ccccagcgtg ttgccggtga aagttcaacg
    61501 ttatccatgt tggttatcca gaatcaaaag gtgggttaat tatcgcatcc gggcagtagt
    61561 attttgcttt tttcagaaaa taatcaaaaa aagttagcgt ggtgaatcga tactttaccg
    61621 gttgaatttg catcaatttc attcaggaat gcgattccac tcacaatatt cccgccatat
    61681 aaaccaagat ttaacctttt gagaacattt tccacaccta aaatgctatt tctgcgataa
    61741 tagcaaccgt ttcgtgacag gaatcacgga gttttttgtc aaatatgaat ttctccagat
    61801 acgtaaatct atgagccttg tcgcggttaa cacccccaaa aagactttac tattcaggca
    61861 atacatattg gctaaggagc agtgaaatgc gcgttaacaa tggtttgacc ccgcaagaac
    61921 tcgaggctta tggtatcagt gacgtacatg atatcgttta caacccaagc tacgacctgc
    61981 tgtatcagga agagctcgat ccgagcctga caggttatga gcgcggggtg ttaactaatc
    62041 tgggtgccgt tgccgtcgat accgggatct tcaccggtcg ttcaccaaaa gataagtata
    62101 tcgtccgtga cgataccact cgcgatactt tctggtgggc agacaaaggc aaaggtaaga
    62161 acgacaacaa acctctctct ccggaaacct ggcagcatct gaaaggcctg gtgaccaggc
    62221 agctttccgg caaacgtctg ttcgttgtcg acgctttctg tggtgcgaac ccggatactc
    62281 gtctttccgt ccgtttcatc accgaagtgg cctggcaggc gcattttgtc aaaaacatgt
    62341 ttattcgccc gagcgatgaa gagctggcag gtttcaaacc agactttatc gttatgaacg
    62401 gcgcgaagtg cactaacccg cagtggaaag aacagggtct gaactccgaa aacttcgtgg
    62461 cgtttaacct gaccgagcgc atgcagctga ttggcggcac ctggtacggc ggcgaaatga
    62521 agaaagggat gttctcgatg atgaactacc tgctgccgct gaaaggtatc gcttctatgc
    62581 actgctccgc caacgttggc gagaaaggcg atgttgcggt gttcttcggc ctttccggca
    62641 ccggtaaaac caccctttcc accgacccga aacgccgcct gattggcgat gacgaacacg
    62701 gctgggacga tgacggcgtg tttaatttcg aaggcggctg ctacgcaaaa actatcaagc
    62761 tgtcgaaaga agcggaacct gaaatctaca acgctatccg tcgtgatgcg ctgctggaaa
    62821 acgtcaccgt gcgtgaagat ggcactatcg actttgatga tggttcaaaa accgagaaca
    62881 cccgcgtttc ttatccaatc tatcacatcg ataacatcgt taagccggtt tccaaagcgg
    62941 gccacgcgac taaggttatc ttcctgactg ctgatgcttt cggcgtgttg ccgccggttt
    63001 ctcgcctgac tgccgatcaa acccagtatc acttcctctc tggcttcacc gccaaactgg
    63061 ccggtactga gcgtggcatc accgaaccga cgccaacctt ctccgcttgc ttcggcgcgg
    63121 cgttcctgtc gctgcacccg actcagtacg cagaagtgct ggtgaaacgt atgcaggcgg
    63181 cgggcgcgca ggcttatctg gttaacactg gctggaacgg cactggcaaa cgtatctcga
    63241 ttaaagatac ccgcgccatt atcgacgcca tcctcaacgg ttcactggat aatgcagaaa
    63301 ccttcactct gccgatgttt aacctggcga tcccaaccga actgccgggc gtagacacga
    63361 agattctcga tccgcgtaac acctacgctt ctccggaaca gtggcaggaa aaagccgaaa
    63421 ccctggcgaa actgtttatc gacaacttcg ataaatacac cgacacccct gcgggtgccg
    63481 cgctggtagc ggctggtccg aaactgtaat gatttgaagc tggagaatat ctatccagta
    63541 tcttatagaa agcaaaacgg gaggcacctt cgcctcccgt ttatttaccc ttcttttgtc
    63601 gtgccctgcg cccgcgttac cggcactggc agccaggcgc gaatggaaag cccgccccgc
    63661 tcgctggtgc caagctccag catcccgtta tggttatcca cgatacgctg cacaattgcc
    63721 agccctaatc ccgtgccgct aatggtgcgc gcactgtcgc cgcggacaaa cggctggaac
    63781 aggtgcttac gttgttccgg cgcaattccc ggaccgtcat cttccacctg gaaccaggcg
    63841 cgattcggct ccgttccgct gctgactttg atccagccat tgccataacg ggcggcgttg
    63901 accaccatat tcgccaccgc gcgtttgatc gacagcgggt gcattttcac ttcaatgctg
    63961 ccggggtaaa gcgcggtttc aatttcccgc tcatagccac tttcggcagc aatcacctca
    64021 ccgagcactg cattaagatc cgccatttcc atcggcatct cctgcccggt gcgcaggtag
    64081 tcgataaact gctcaatgat ggcgttgcac tcttcgatat ctttattgat cgattctgcc
    64141 agatagccat cctgctcgct catcatctca gtcgccaggc gaatacgcgt cagcggcgtg
    64201 cgcaagtcgt gacttacccc cgccatcagc agcgtgcggt catccgccag ttgcttaaca
    64261 ccagccgcca tatggttaaa ggcacgggta acggaacgca cctccgaagc gccatattca
    64321 cgcagcggcg gcggaataat ccctttacca acctgcaagg ctgcgtgttc gagatcgacc
    64381 aacggtcggt tctggatacg aataaacagc cacgccccgc ctatcgccaa tagcataatc
    64441 gccagcgtat agcggaacag cggagagaaa tcgccctgat gaatttcggt cagcggcacg
    64501 cgtacccaga tattgggcga cagccaggtt ttcagccaga cgacaggcga acttttgttg
    64561 acctcaacgc gcacttccgt cgggccgccc agttgctgtg ccatctgatg gcttaagaat
    64621 tcatagtgtt gcgcccaacg cagacctgcc tcttcggcag cctcgttgga gtagagagag
    64681 atccccagct cacggtagat ctcccgacgg aaagcgggag gcacaaccaa ctgcgtgccg
    64741 tcctccagtt gcagtttatc ggtcatcaac atacgcactt cgtacgcgag gactttatta
    64801 aactgctgga ggctcggcaa aatcgcgaag ttcagcacca ccagataagt cgtcaccagg
    64861 ctggcgaaca gcaaggtgac gatgagcaat aacgtacggg caaatgaact tcgtggcgag
    64921 aagcgcaatc gcctcatgct ttagagccgt ccggtacaaa gacgtagccc aggccccaaa
    64981 cggtctgaat gtaacgcgga tgcgctggat cttcttctac catgcggcgc agacgcgaaa
    65041 tctgcacgtc gatggagcgt tccattgcgg aatattcacg accacgggca aggttcatca
    65101 gcttatcgcg ggagagcggc tcacgcggat ggctgaccag tgccttcagt accgcaaact
    65161 caccgctggt gagcggcatc ggctcatctt cgcggaacat ttcgcgcgta ccgaggttaa
    65221 gtttgaactt accgaaagca attaccgcct cttcctgtga cggtgcgcct ggcagttcgt
    65281 tcgcctgacg acgcagcacc gcacgaatac gggccagcag ttcacgcggg ttaaacggtt
    65341 ttggaatgta gtcgtcagcg ccaatctcca ggcctacgat acggtccact tcttcccctt
    65401 tcgccgtcac cataatgatc ggcatcgggt tgctctgact acgaagacgt cggcaaatcg
    65461 acaagccatc ttcaccaggt aacattaaat ccagtaccat aagatggaaa gattcacgag
    65521 tcagcaggcg atccatctgt tctgcattag cgacgcttcg aacctggaag ccttgttcgg
    65581 tgagataacg ttccagcagc gcacgcaggc gcatgtcgtc atcgaccacc agaatcttgt
    65641 agttctcttg cattgtttgt actcccaaag gttcgcaaca atttgtaagc gtgtattctt
    65701 aaaaaagctc acgttcgtca ccagctaaat ctggtatgaa tttcagccta aattgttaca
    65761 aagcatatta aacagcagct taagtataca atttattcgg cgaaacatta ttgattctgt
    65821 tgatatgatc acgttatacc caatgtgcgc attatcaaac agacaaaggg aatcaacgag
    65881 atgaaaacgc ccctggttac ccgggaaggg tatgaaaaac tcaaacaaga gcttaattat
    65941 ctctggcgtg aagaacgccc ggaggtcaca aaaaaggtga cctgggccgc aagtctgggc
    66001 gaccgcagcg aaaatgctga ctatcagtat aataaaaagc gtctgcgtga aatcgaccgt
    66061 cgcgtgcgct atctcactaa atgcctggaa aatctcaaaa ttgtcgatta ctcccctcag
    66121 caggaaggca aagtcttttt tggcgcgtgg gtggagattg aaaacgacga tggcgtgact
    66181 caccgtttcc gtattgtcgg ctacgatgaa atttttggcc gtaaagatta catctctatc
    66241 gattccccga tggcccgcgc attgctgaaa aaagaagtcg gcgatctggc ggtggtgaat
    66301 acccctgccg gggaagcgaa ctggtatgtt aatgctatcg agtacgtgaa accgtaagga
    66361 agagtcttaa cctcctgccg atggctggca tttttgccag ccagtccgta taactatccc
    66421 ctgatttttg atccgaaaag atgaactcaa accatgatga atgattcgtt ctgccgcatt
    66481 attgcgggtg aaattcaggc gcgcccggaa caggttgacg ctgccgttcg cctgcttgat
    66541 gaagggaata ccgtgccgtt tatcgcacgt tatcgtaagg aaatcaccgg cggtctggat
    66601 gacacgcagc tgcgtaatct ggaaacgcgt ctgagctatc tgcgcgagct ggaagagaga
    66661 cgtcaggcga tcctcaaatc catttccgag caaggcaaac tcaccgatga tctggcgaag
    66721 gccatcaacg ccaccctaag caaaaccgaa ctcgaagacc tctacctgcc ctacaaacct
    66781 aaacgccgca cccgcgggca aatcgccatt gaagcagggc ttgagccgtt ggctgacctg
    66841 ctgtggagcg atccgtcaca cacgccagaa gtcgccgctg cacaatatgt tgatgccgat
    66901 aaaggcgtgg cagataccaa agccgcgctg gatggcgcgc gctatatcct gatggaacgg
    66961 tttgccgaag atgccgcgct gctggcgaaa gtgcgtgatt atctgtggaa gaacgcgcat
    67021 ttggtttcta cggtggtgag cggtaaagaa gaggaagggg cgaaattccg cgactatttc
    67081 gatcatcacg aaccgttgtc cacggtgcct tctcaccgcg cgctggcgat gttccgtggg
    67141 cgtaacgaag gcgtactcca gctttcgctg aatgccgatc cgcagttcga tgagccgccc
    67201 aaagagagct attgcgagca aatcatcatg gatcaccttg gcctgcgcct gaacaatgcc
    67261 ccggcggata gctggcgcaa aagcgtagtg agctggacct ggcgcatcaa ggtgctgatg
    67321 catctggaaa ccgaactgat gggtaccgtg cgcgaacgcg cggaagatga agcaatcaac
    67381 gtctttgccc gtaacctgca cgatctgctg atggcggccc ctgccggact gcgtgcaacg
    67441 atgggcctcg atccgggtct gcgtaccggg gtaaaagtgg cggtggtcga tgccactggc
    67501 aaactggtgg cgaccgatac catttacccg cacaccggac aagccgcaaa agcagcgatg
    67561 accgttgctg ccttgtgtga aaaacataac gttgaactgg tggcgatcgg caacggtaca
    67621 gcttcccgcg aaactgaacg tttctatctc gacgtgcaga agcagttccc gaaagtgacc
    67681 gcacagaaag tgatcgtcag cgaagctggc gcgtcggttt actcagcttc cgagctggca
    67741 gcgcaggagt tcccggatct cgacgtttcg ctgcgtggtg cggtatctat cgcccgccgt
    67801 ttgcaggatc cgctggcgga gctggtgaaa atcgatccga aatctatcgg cgtaggtcag
    67861 tatcagcatg acgtcagcca gacgcaactg gcccgcaaac tggatgcagt agtagaagac
    67921 tgcgtaaacg gcgttggcgt cgatctcaac accgcttctg ttccgctatt aacccgcgtg
    67981 gcgggcctga cgcgcatgat ggcgcaaaac atcgtggcct ggcgcgatga gaacggccag
    68041 ttccagaacc gtcagcaact gctgaaagtc agccgtctgg ggccgaaagc cttcgagcag
    68101 tgcgcgggct tcctgcgcat taaccacggt gataacccgc tggacgcctc taccgttcat
    68161 ccggaagcct atccggtggt ggaacgcatt ctggcagcaa cacagcaggc actgaaagat
    68221 ctgatgggta acagcagcga actgcgtaac ctgaaagcgt ctgactttac tgatgaaaaa
    68281 ttcggtgtgc cgacagtaac tgacatcatc aaagagctgg aaaaaccggg tcgcgatccg
    68341 cgtccggaat ttaaaaccgc tcagtttgcc gatggcgtcg agacaatgaa cgacctgcaa
    68401 ccgggtatga tcctcgaagg cgcagtgacc aacgtcacca actttggcgc gtttgtcgat
    68461 attggcgtgc atcaggacgg cctggttcac atctcttcat tgtcgaacaa gtttgtggaa
    68521 gatccgcata ccgtggtgaa agcgggcgac attgtgaagg tgaaagtgct ggaagtggat
    68581 cttcagcgta aacgtatcgc cctgactatg cgtctggacg agcagcctgg cgaaaccaac
    68641 tctcgtcgcg gcggcggtaa tgaacgcccg caaaacaacc gcccggcagc caaaccacgc
    68701 ggtcgtgaag cgcagcctgc cggtaatagc gcgatgatgg atgcgctggc ggcagcgatg
    68761 ggtaaaaaac gttaaacgcc cggactggcc cctaaacctg cgcgataagg cgttcacgcc
    68821 gcatctggca acgaacgctt tgcctgacat aaaagtgccg gagaatatct ccggcacttt
    68881 tattccacag ccaaactcac aatatactcc gttaatattt atcacttcat taacaactga
    68941 aaccttaatt aaacattagc cagtccgggt aactcactat tcgaattata ttttcgctgc
    69001 gatataacct tgagccacat caacattgag tcagattatt attcaaacca acattcgtac
    69061 acattttaag tattgctgat agaaaccatt ctcattatca ttgtgttgtt gattatttaa
    69121 tctctccttt gttggcaaat catctggtct catgtcgcgg tcaaacgccc catgaggtag
    69181 ttatccagtt aatgagaaac aagtaggcac ctatgcaata cactccagat actgcgtgga
    69241 aaatcactgg cttttcccgt gaaatcagcc cggcatatcg ccaaaaactg ctttctctag
    69301 gcatgttacc tggctcctct tttgatgtgg tgcgcgtcgc tccactcggc gaccccattc
    69361 atatcgaaac ccgtcgtgtg agcctggtat tacgcaaaaa agatctggcc ttattagaag
    69421 tggaagcggt ttcctgttaa tacggtgata acaacaatga aaaaattaac cattggctta
    69481 attggtaatc caaattctgg caagacaacc ttatttaacc agctcactgg cgcacgtcag
    69541 cgtgtaggta actgggctgg cgttaccgtc gaacgtaaag aagggcaatt ctccaccacc
    69601 gatcatcagg tcacgctggt ggacctgccc ggcacctatt ctctgaccac catctcatcg
    69661 cagacctcgc tcgatgagca aatcgcctgt cactacattt tgagtggcga cgccgacctg
    69721 ctgattaacg tggtggatgc gtctaacctt gagcgtaacc tgtacctgac gctgcaactg
    69781 ctggaactcg gcattccctg catcgtggcg ctgaacatgc tcgacattgc cgagaagcaa
    69841 aatattcgta ttgaaattga tgctctatcg gcacgtcttg gctgcccggt gatcccgctg
    69901 gtttcaaccc gtggtcgcgg tattgaagcg ctcaagctgg cgattgatcg ctataaagct
    69961 aacgagaatg tggaactggt gcattacgca cagccgctac tcaacgaagc agattcactg
    70021 gcaaaagtga tgccttccga catcccgctg aaacaacgtc gctggctggg cctgcaaatg
    70081 ctggaaggcg atatctacag ccgcgcctac gccggtgaag cgtcgcagca tctggatgcc
    70141 accctcgccc gtctgcgtaa tgaaatggat gatccggcgc tgcacattgc cgatgcgcgt
    70201 taccagtgca ttgctgccat ctgtgatgtg gtaagcaaca ccctgacggc agaacccagc
    70261 cgtttcacca ctgcggtaga taaaatcgtg ctcaaccgtt tcctcggtct gccgattttc
    70321 ctctttgtga tgtacctgat gttcctgctg gctatcaaca tcggcggggc gttacagccg
    70381 ctgtttgacg tcggctccgt ggcgctattt gtgcatggta ttcaatggat tggctacacg
    70441 ctccacttcc cggactggct gactatcttc ctcgcccagg gcctgggtgg cggtattaac
    70501 accgtgctgc cactggtgcc gcagatcggc atgatgtacc tgttcctctc cttccttgag
    70561 gactccggct atatggcgcg tgcagcgttt gtgatggacc gcctgatgca ggcgctgggc
    70621 ttgccgggga aatcctttgt gccgctgatc gtcggtttcg gttgtaacgt accgtcggta
    70681 atgggcgcac gtacgcttga tgcaccgcgt gaacgtctga tgaccatcat gatggcaccg
    70741 tttatgtcct gcggcgcgcg tctggctatc ttcgcagtat tcgcggctgc cttcttcggg
    70801 cagaacggtg ccctggcggt tttctcgctg tatatgctcg gtatcgtgat ggcggtgctg
    70861 actggcctga tgctcaagta caccatcatg cgcggtgaag caacgccgtt tgtcatggag
    70921 ctgccggtct accatgtacc acacgttaaa agcctgatta tccagacctg gcagcgtctg
    70981 aagggcttcg ttctgcgtgc tggtaaagtg attatcatcg tcagcatttt cctgagcgct
    71041 ttcaacagct tctcgttgag cgggaaaatc gtcgataaca tcaacgactc ggcgctggct
    71101 tccgtcagcc gggtgataac ccctgtcttc aagccgattg gcgtacatga agataactgg
    71161 caggcaacgg ttggcctgtt tacgggcgca atggcaaaag aagtggtggt gggtacgctc
    71221 aacaccctct acaccgcaga aaatattcag gacgaagagt tcaatccggc ggaattcaac
    71281 ctcggtgaag agctgttcag tgcggtagat gaaacctggc agagcctgaa agacaccttc
    71341 agccttagcg tactgatgaa ccccattgaa gctagcaaag gcgacggtga aatgggtacc
    71401 ggggcgatgg gcgtgatgga tcagaaattc ggtagcgcag ctgccgcata cagctacctg
    71461 attttcgtcc tgctgtatgt accatgtatc tcggtgatgg gagccatcgc ccgtgaatca
    71521 agccgtggct ggatgggctt ctccatcctg tgggggctga atatcgctta ctcactggca
    71581 acattgttct atcaggtcgc cagctacagt cagcacccaa cttacagcct ggtgtgcatt
    71641 ctggcggtaa tcctgtttaa catcgtagtt atcggtctgc tgcgccgcgc gcgtagccgg
    71701 gtggatatcg aactgctggc aacccgcaag tcggtaagca gttgctgcgc agccagcacc
    71761 accggtgatt gccattaatg gcttcactta ttcaggtacg tgatttgctg gcgttacggt
    71821 gccgtatgga agcgacccag ataagccaga cattgaacac tccgcagcca atgattaacg
    71881 ccatgctgca acagctggaa agcatgggca aagccgtgcg gattcaggaa gaacctgacg
    71941 gctgcctctc tggcagttgt aaaagctgcc cggaaggcaa agcctgtctg cgcgagtggt
    72001 gggcgctgcg ttaaccatac tccatcgcct gttttgaaaa gcccggtatg cgtttgcatc
    72061 cgggcttttt tgcgtgcggc tttccataaa aatgcaattc ttgcagcgca acgtaagttc
    72121 ctctgaaagc gtctcgcagg gatgaaaact cgctaatgca cagatgtgga gtggcgcgta
    72181 gagtcgcggc attcaaacaa caggtgaagg aacgccatga gtaaaaagca gagttccacc
    72241 ccacatgatg cgctgttcaa actcttttta cgccaaccgg agacggcacg cgattttctt
    72301 gcgtttcatt taccggcacc cattcacgcg ctctgtgata tgaaaaccct caagctggag
    72361 tcgagcagct ttattgatga cgatctgcgt gaaagctatt ccgatgtgct gtggtcggtg
    72421 aaaacggaac aaggaccagg atacatctat tgtctgattg aacatcaaag cacctcaaac
    72481 aaactgatcg catttcgcat gatgcgttac gctattgccg caatgcaaaa tcaccttgat
    72541 gctggataca aaacgttgcc gatggtggtg ccattgttgt tttaccacgg tattgaaagc
    72601 ccctatccct attcgctgtg ttggctggat tgtttcgccg atcccaaact ggcaaggcag
    72661 ctttatgcct ccgcatttcc gctgattgat gtcaccgtca tgcctgatga tgaaatcatg
    72721 cagcaccgac gtatggcgct gctggagtta attcaaaaac atattcgtca acgcgatctg
    72781 atggggctgg tagagcaaat ggcctgctta ttaagtagtg gatacgctaa tgacagacaa
    72841 atcaaagggc tgtttaatta catactgcaa actggcgacg ctgtacgttt taacgatttt
    72901 atcgacggcg ttgccgaacg ttcaccgaaa cacaaggaga gtttaatgac tattgcggaa
    72961 agattgcggc aggaggggga acaatccaaa gccctgcata tagccaaaat aatgcttgaa
    73021 tccggagtcc ctcttgcaga catcatgcga tttaccgggc tgtcagaaga agagttggct
    73081 gcggcgagtc agtagatact ctaccctgct taagtgctac tttgtgctat cttgcatgta
    73141 acacaaagta gcacttaggg aactgatatg cagacagtga aattacggca acaaggagga
    73201 gccatgatcg ttactatccc acgagatctt gccatcgatc ttggctggtc accgggcaca
    73261 gagcttaccg tggagaaaaa aggcgatagc gttaatttgc gcgccacaga gcacaaaccg
    73321 cgtgggcgtc ttaccgtcgc gcagttgttg agccaaatcg atgaaagcga aattacggag
    73381 ctaaatcagt ccacagaagg atgggctgaa gggaaaaaag gtaatgaagc atggtaaagg
    73441 cacggacgcc acatcgtggt gagatctggt attttaaccc tgatccggtt gccgggcatg
    73501 aacttcaggg gccacattat tgcattgtgg taacggacaa aaaactcaac aatgttttaa
    73561 aagttgctat gtgctgcccg atttcaacag gggcaaatgc agcacgttcc acaggggtga
    73621 cggtgaacgt cctcccctgt gatacgcaaa ccggtaacct gcatggcgtt gtactttgtc
    73681 accagctaaa agccgtcgat cttattgccc gtggcgctaa atttcatacc gttgccgatg
    73741 aaaaattgat tagtgaagtt atcagtaaac tggtgaattt aatcgaccca caataattag
    73801 ctctcactta ccaccaacac cctctgcttc aacgccacca gcaggtgaca aaactcgacc
    73861 ggatgcgaaa taaacggcgc atgggccgct ttggcgaaga tatatgattc gctgtgaggc
    73921 caaagtttat ccagcatcgg caccactttg cgcggcacca gaccgtcgag atagccatac
    73981 aatcgcaaaa acggcatgga cacgttttgc agcggctgac ggagatcgac cgttttcagg
    74041 atttccagcc cgccattaag cacgtcaacc tccggcatcg gtaacgccag aacggttttc
    74101 ttcaacgccc gcgcatcctg gcgcgccgtt tcagtcccca tggtttgtaa cgccaggaac
    74161 cgctccactg tacgctgaaa atcatcactg agttgctgct gaaatcccgc cagcacgtcc
    74221 ggttttatcc ccggccactc gtcacgagca ctaaaataag gtgacgacgc cacggtgacc
    74281 agcgcctgaa cacgctcggg atgggttaac gcaatctggc ttgccaccag cccgcccaga
    74341 ctccagccta accaaatggc tttatcaggt gcctgttgca gcacggcttc ggccatatca
    74401 gcaagtgaca gcgcaccaaa tccccggcta cgcccgaagc cgggcaggtc aacaaggtgc
    74461 agcgtaaaat gcgagctaag ttcctcgtca atgcaacgcc acacttcggc attcagtccc
    74521 catccgtgca gcagcacaag atgaacattc ccctgacctt tggtctgcca ccagatgtta
    74581 ttcatccgct attgttctct tttgacttac aaggatgaac atatgctaac agtaccggga
    74641 ttatgctggc tatgccgaat gccactggcg ttaggtcatt gggggatttg ttcggtctgt
    74701 tcacgcgcca cccgcacaga taaaacgtta tgcccacaat gtggattacc cgccacacac
    74761 tcccatcttc cctgcggtcg ctgcctgcaa aaaccgccgc cctggcaaag actggtcacg
    74821 gttgccgact atgcgccgcc gttaagtccg cttatccacc agcttaaatt ttcccggcgc
    74881 agtgaaatcg ccagcgccct gtcacgtctg ttactattgg aagtcttaca cgctcgtcgc
    74941 accaccgggt tgcaattgcc ggatcgcatc atcagcgttc cgttatggca gcggcgtcac
    75001 tggcgtcggg gatttaatca gagcgatttg ctgtgtcagc cgttatcacg ctggttgcac
    75061 tgccaatggg atagcgaagc cgtcacacgt acacgggcca ctgcgaccca gcattttctc
    75121 agtgcccggc tgcgcaagcg caacctgaaa aatgcctttc gtcttgaatt gcccgtgcaa
    75181 ggtcgccata tggtgattgt ggatgatgtc gttaccaccg gaagtaccgt cgcagagatt
    75241 gcgcagttgc ttttacgcaa tggtgcggcg actgtccagg tctggtgcct ttgtcgaacc
    75301 ttgtagagcc tcgatgatgg gcgtattata accaactaaa atagtcaact attaggccat
    75361 tactatgatc cgtatttccg atgctgcaca agcgcacttt gccaaactgc tggcaaatca
    75421 ggaagaaggg acacaaatcc gcgtatttgt gattaaccct ggcacgccta acgctgaatg
    75481 tggtgtttct tattgtccgc cggatgccgt ggaagccacc gacactgccc tgaaatttga
    75541 cctgctgacc gcgtatgttg atgagttaag cgcaccatac ctggaagatg cagagatcga
    75601 ctttgttacc gaccagttgg gttctcagtt aacgctgaaa gccccgaacg ccaaaatgcg
    75661 taaagtggca gacgatgcac cgctgatgga gcgcgtggag tatatgctgc agtcgcagat
    75721 caacccacag cttgctggtc acggtggtcg cgtttcgctg atggaaatca ccgaagacgg
    75781 ttacgccatt ctgcaatttg gcggcggctg taacggttgt tccatggtcg atgtgacgct
    75841 gaaagaaggg atcgagaagc agctgctgaa cgaattcccg gagctgaaag gtgtgcgcga
    75901 tctcaccgaa caccagcgcg gcgaacactc ctactactaa gttatcccct cttttgtaga
    75961 ttgcccgatg cgacgctaaa gcgtcatttc gggcctacgt tgaacatgtg ccagacgttg
    76021 gcacagcatt gctttggcaa atccctccct tcgtttcccc gcgataatat gaccaacctc
    76081 tcataattta aatttacccc gctctggtga ttctcaaacg ccagatgtta cccgtatcat
    76141 tcacatgggt accaaacata ctcctgacat ctgactacaa taattagttt tagtgggtat
    76201 cagtcgtggt gccgcaatat ctctgttccc gattgggata attagagttt gtcgtcagaa
    76261 aattgacgtt acccataaca aatgaaaggc caggtaaatc atgccattag tcattgttgc
    76321 tatcggtgta atcttgttgt tgctcctgat gatccgcttc aaaatgaacg gcttcatcgc
    76381 tctcgtcctc gtggcgcttg ctgttggatt aatgcaagga atgccgctgg ataaagttat
    76441 tggctccatc aaagccggtg tcggcgggac gctcggtagc cttgccctga tcatgggttt
    76501 tggcgcaatg ctggcaaact gcggtggcgc acaacgtatc gccaccacgc tgattgccaa
    76561 atttggtaaa aagcacatcc agtgggcggt ggtgttaacc ggttttaccg ttggttttgc
    76621 cctgttctat gaagtgggct tcgtgctgat gctgccgctg gtgtttacca tcgcggcttc
    76681 cgcgaatatt ccattgctgt atgttggtgt accaatggcg gctgcactgt ctgtgaccca
    76741 cggcttcctg ccaccgcatc cgggcccgac tgcgattgcc accattttca atgccgatat
    76801 gggtaaaacc ctgctgtacg gtactattct ggcaatcccg accgtgattc tcgccggtcc
    76861 ggtttacgct cgcgtgttga aaggtatcga taagccaatc ccggaaggtc tctacagcgc
    76921 gaaaaccttc agcgaagaag agatgccgag ctttggcgtc agcgtctgga cctctctggt
    76981 gccggttgtg ctgatggcga tgcgtgcgat tgccgaaatg atcctgccga aaggtcacgc
    77041 tttcctgccg gtagcggagt tcctcggtga cccggtaatg gcaacgctga ttgccgtgct
    77101 gattgcgatg ttcacctttg gtctgaaccg tggtcgttca atggatcaga ttaacgacac
    77161 gctggtttct tccatcaaaa tcattgcgat gatgctgttg atcatcggtg gtggcggtgc
    77221 gttcaagcag gtgctggtag acagcggcgt ggacaaatac attgcttcca tgatgcacga
    77281 aaccaacatt tctccgctgc tgatggcctg gtcgattgct gccgtactgc gtatcgcgct
    77341 gggttccgca accgttgcgg caatcactgc gggtggtatc gcggcaccgc tgattgcaac
    77401 gacgggtgtt agcccagagc tgatggttat tgcggtgggt tccggtagtg tgattttctc
    77461 tcatgtgaac gatccgggct tctggctgtt caaagagtac tttaacctga ctatcggcga
    77521 gaccatcaaa tcctggtcga tgctggaaac cattatctcg gtgtgcggtc tggtaggctg
    77581 tctgctgctg aatatggtga tttgataaca ctgactgccg gatgcatcgc atccgacatc
    77641 aaccgcactc tacttcttct tcgctgcagc tctgcgccgt ctgtccaaat ccttcagcaa
    77701 cttgttcacg ccatcatcgg caaacatcga ctcaagcgtt gcggaaagct tgcgtcgcca
    77761 gtttttatac tggtaactgg tgccaggaat attcaccggt tcggccatat ccagccagtc
    77821 ttccggctgt agtcctaaca gagcactgtt actgtcggca atgtagcgct gcaaaccacg
    77881 gttcagcgtc ggcgtcatcg acatcaacga tgccttatgc ccggcacgtt ttggcagaca
    77941 accatattta tgcagtgcat ccagcagccc ttgcttcgcc agttcgcgat cctgatacag
    78001 accgcgcagt accacttcat ccggatacag ccccagggtt ttgcccagcg ttagatcccc
    78061 gctctcccag taaccacgca gcgttggcag gtcatgtgtc gccgcaaccg ccatcgactg
    78121 ctccggatac gctttcggtg cacggaacgt cttctcgtgg tcgttttcga aatagagcac
    78181 tttgtaagag tacacaccgc tgctgcgcag cttaccgaca atctctaccg gtacggtacc
    78241 gagatcttca ccaatcacca tacagcgatg acgtttactt tcgagtgcca gaatcgagag
    78301 cagatcatcc accggatagt gaacatacgc gccctgatct gccgtctcgc catacggtat
    78361 ccaccacaaa cgcagcatcg acatcacatg gtcaattcgt aatgcgccgc agttttgcat
    78421 attggcacgc aacagctcga taaacggttc ataggcacgc gcggtgatga tatgcgggtc
    78481 cattggcggt aatccccagt tctgccccaa cgggccgagg atatccggcg gcgcgccaac
    78541 cgatgctttc aggcaatata gttcacggtc acaccaggtt tccgccccac cttccgctac
    78601 gccaaccgcc agatcacgat acaagccaat cggcatttca tagccctggc ttatctccca
    78661 gcaggcggca aactggctgt aagccagcca ctgcaaccag agataaaaat cgacgtcatc
    78721 acgatgttct tcgcagaact gacgcacttc tggtgaatcc acgttcttat acatctctgg
    78781 ccatgcaggc cagccccagc gcatttcgtc ctctttcact tgctgggcat gtagcgcatc
    78841 aaaggctgcc tgccagaaca ggctgtcgcc ctgctctgca acaaactggc gaaacgcggc
    78901 catctgctca tcatcacgtt gcgcgaaacc tttccacgcc attcgtaatg ctgtcatttt
    78961 tagggcggta accgtggagt aatcgaccca gtcggcatcg cgcgcctgtt gcagcgtctg
    79021 ttgcgtggtc ggcaactgcc accaggcctg agcctcttcg ctaagatgga aatcttcaac
    79081 ggcgttaacg tcgatataaa tcacattcag ccaacggcga gaagacgggc tgtatgggct
    79141 ggcgctctcc ggatttgccg gatagagcgc atgaatcggg ttcaggccaa tgaacgaccc
    79201 gccacgtttt gccacatcca ccagcatcgc tttgagatcg ccaaaatccc caatacccca
    79261 gtttttttcc gatcgcagcg tataaagctg aacgcaggca ccccacagct tttgtttatt
    79321 cagcaaagcc tgcggttcgt aacaacgttt cggggcgaca atcacccggc aatgcgcgcg
    79381 ctggtcgtcc tgggtgagtg ttagcgtgtg ataaccttcc ggcagcttcg tcggtagatt
    79441 gaacgctttg ccccccgtta catggccttt gtactgcgtt ccttcttcgg tggtcagctg
    79501 ccagctatat tcgccgctgc cctccaccac catcggcatt tttttgccgc tggtataaac
    79561 catgacattc gggactggcg ttaccgccac tttcgtggcg gtacgttgat gcatcgcgtc
    79621 aagcaaacgc cgtttggttt cggcgctaat cgactgcggt ttaccgtggg cattgatgta
    79681 attggggcta atccccgccg ccagcgcggc attatccaga cgtttgcttt ccatcgagct
    79741 tccttagcgt tttgcctgcc agatacgagc ctgataatcg cgaatagagc gatccgagct
    79801 aaacataccg cagcgggcgg tattgaggat cgccgcgcga gtccaggcct cctggtcgcg
    79861 gtacagcaca tccacctgct tttgtgcctc aacataggct gcgaaatccg ccatcaccag
    79921 atacggatcg ccgccctgtt tgccgatact gtgcagcatc tggtcgaagg catgcttatc
    79981 gccgtcgctg tatttaccgc tttccagctc tttcaatact gcgtccagca ccttatcttt
    80041 cttccgccat ttcaccgggt cgtagccttt ggccagaata gccttcactt gttccacggt
    80101 atgaccaaaa ataaagatat tttcttcacc gactttctcg gcgatttcaa cgttcgcacc
    80161 atccagcgta ccgacagtaa gcgcaccatt gagcgccagt ttcatattgc cggtaccgga
    80221 agcttcttta cctgcagtcg aaatttgttc ggagatatcc gccgccggga tcagtttttc
    80281 cgccgccgaa acgcaataat ccggcaggaa caccaccttc aacttatcgc caaccagtgg
    80341 atcgttgttg atcacgtcag ccactttgtt gatcgcaaag ataatattct tcgccaggta
    80401 gtagcccggt gccgctttcg cgccgaagag gaagacgcgc ggtacgcgat cagcctgcag
    80461 gttttcacga atttctttgt acaacgccag aatatgcagc agattcaggt gctggcgttt
    80521 gtactcatgc agacgtttga tctgaatatc gaaaatcgcc tgtggattga tctcaatacc
    80581 ggtacgaact ttcacaaact ccgccagacg gactttattc gcctgcttga tctcgcgata
    80641 ttgctgacgg aatttcgcat catcagcgaa tttttccaga ttgatcagct gatcgagatc
    80701 gttagcccac tctttttgca gtgatttatc caacagagcc gccagtgcag ggttgcactg
    80761 tttgatccag cgacgtgggg taataccgtt ggtgacgtta tggaatttgt tcggccatag
    80821 ctggtgatat tccgggaaca gatctttcac caccagatcc gagtgcagcg ccgcaacacc
    80881 gttcaccgcg aaaccgccaa ccacacacag gttcgccata tgcacttgtt tatcgtgcac
    80941 caccgccagt ttggcccaca ctttttcatc gcccggccag gttttctcca ccagcgtttt
    81001 aaagcgagta ttaatttcgt taataatctg catatggcgc ggcagtaagc ctttcaccag
    81061 tttcacatcc cagcgttcca gcgcttctgg catcagggta tggttggtgt aagcgaaagt
    81121 tttgctggtg atggcccagg cgtcatccca gctcatctgg tgctcatcgg tcagcacgcg
    81181 cagcagttct ggaatcgcga tagttggatg ggtatcgttc agctgaataa cttcgtaatc
    81241 cgccagttcg tgcagtttac gccccgccag atgatggcga cgcaaaatat ccgctaccga
    81301 acaggcacac tggaagtatt gctgcatcag gcgcagcttt ttaccggcag tatggttgtc
    81361 gtttggatag agaactttgg tcagtttttc cgcattgatg ccttgctgtt cggcgcgcag
    81421 gaaatcaccg tcgttaaatt tagttagatc aaacggatgc gcatgcgtcg cctgccacag
    81481 acgcagcggc tgcgccacgc cattacgata gccgacaacg gggagatccc acgcttgacc
    81541 ggtaatggta aactccggct cccagcgtcc gtctttcatc actttaccgc caatccctac
    81601 ctgcacatcc agtgcttcgt tgtggcggaa ccacgggtag ttactgcgat gccagtcatc
    81661 cggcgcttca acctgtttgc catcgacaaa agactggcgg aacaaaccat attgatagtt
    81721 cagaccgtaa cccgtcgcag actgaccgac agttgccatt gagtcgagga agcacgccgc
    81781 cagacgtccc agaccaccgt tacccagcgc cgggtcgatc tcttcttcca gcaggtcagt
    81841 caggttgatg tcataagcct tcaacgaatc ctgtacatcc tgataccagc cgagattcaa
    81901 caggttgttg cccgtcagac gaccaatcaa aaactccatt gagatgtagt taacatgtcg
    81961 ctgattcgcc accggtttgg cgaatggctg agcacgcagc atttcggcca gtgcttcact
    82021 cactgccagc caccactggc gaggagtcat ttcagccgca gaatttaagc cataacgctg
    82081 ccactgacgt gaaagcgctt cctgaaattg cttatcgtta aaaataggtt gtgacatagg
    82141 agttccactt ttcttagatt ttcaacacaa cgttatcgct agtttgccag gctcgatgtt
    82201 gaccttcctc atcctgcggg ggattaggca gggaggagtt gcggggatga gcaaggaaat
    82261 gtgatctcga ccacttaaag ctagtgcaaa ccacaggatt agcagcaaat caatgccata
    82321 cagcgcagaa aatctgtatc taagtgcaaa aaatggccgt tgcgtatttt caaaaagcgg
    82381 aaggtaactc tataaattaa gtaaaggagt gaaacagttt cacaagtaaa atatccagtg
    82441 tgctccatct cattcttaat agatttatta agatcatctt tttagatggc actttcatca
    82501 ggaatgaaga agaaaccctt gcttaaatga atctgat
//
LOCUS       DAJTYG010000004        76398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTYG010000004 DAJTYG010000000
VERSION     DAJTYG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 76398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.004"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..535
                     /locus_tag="OO568_000310"
     CDS             <1..535
                     /locus_tag="OO568_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR6639806.1"
                     /translation="NTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLK
                     RVINSFNSELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQR
                     ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQL
                     EIKHSHPKNRASGKRVS"
     gene            955..1570
                     /locus_tag="OO568_000311"
                     /pseudo
     CDS             955..1570
                     /locus_tag="OO568_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1673..2176
                     /locus_tag="OO568_000312"
     CDS             1673..2176
                     /locus_tag="OO568_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCR6639807.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(2243..3700)
                     /gene="nuoN"
                     /locus_tag="OO568_000313"
     CDS             complement(2243..3700)
                     /gene="nuoN"
                     /locus_tag="OO568_000313"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6639808.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3707..5236)
                     /gene="nuoM"
                     /locus_tag="OO568_000314"
     CDS             complement(3707..5236)
                     /gene="nuoM"
                     /locus_tag="OO568_000314"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6639809.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITVATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(5400..7241)
                     /gene="nuoL"
                     /locus_tag="OO568_000315"
     CDS             complement(5400..7241)
                     /gene="nuoL"
                     /locus_tag="OO568_000315"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6639810.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(7238..7540)
                     /gene="nuoK"
                     /locus_tag="OO568_000316"
     CDS             complement(7238..7540)
                     /gene="nuoK"
                     /locus_tag="OO568_000316"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6639811.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7537..8091)
                     /gene="nuoJ"
                     /locus_tag="OO568_000317"
     CDS             complement(7537..8091)
                     /gene="nuoJ"
                     /locus_tag="OO568_000317"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6639812.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(8103..8645)
                     /gene="nuoI"
                     /locus_tag="OO568_000318"
     CDS             complement(8103..8645)
                     /gene="nuoI"
                     /locus_tag="OO568_000318"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6639813.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8660..9637)
                     /gene="nuoH"
                     /locus_tag="OO568_000319"
     CDS             complement(8660..9637)
                     /gene="nuoH"
                     /locus_tag="OO568_000319"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6639814.1"
                     /translation="MSWISPELIEILLTILKAVGILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9634..12360)
                     /gene="nuoG"
                     /locus_tag="OO568_000320"
     CDS             complement(9634..12360)
                     /gene="nuoG"
                     /locus_tag="OO568_000320"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6639815.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(12413..13750)
                     /gene="nuoF"
                     /locus_tag="OO568_000321"
     CDS             complement(12413..13750)
                     /gene="nuoF"
                     /locus_tag="OO568_000321"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6639816.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13747..14247)
                     /gene="nuoE"
                     /locus_tag="OO568_000322"
     CDS             complement(13747..14247)
                     /gene="nuoE"
                     /locus_tag="OO568_000322"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6639817.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(14250..16052)
                     /gene="nuoC"
                     /locus_tag="OO568_000323"
     CDS             complement(14250..16052)
                     /gene="nuoC"
                     /locus_tag="OO568_000323"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6639818.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRTQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(16146..16808)
                     /gene="nuoB"
                     /locus_tag="OO568_000324"
     CDS             complement(16146..16808)
                     /gene="nuoB"
                     /locus_tag="OO568_000324"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6639819.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16824..17267)
                     /gene="nuoA"
                     /locus_tag="OO568_000325"
     CDS             complement(16824..17267)
                     /gene="nuoA"
                     /locus_tag="OO568_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6639820.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRECMNPETNSIANRQR"
     gene            complement(17898..18836)
                     /gene="lrhA"
                     /locus_tag="OO568_000326"
     CDS             complement(17898..18836)
                     /gene="lrhA"
                     /locus_tag="OO568_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6639821.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19761..20978
                     /gene="alaA"
                     /locus_tag="OO568_000327"
     CDS             19761..20978
                     /gene="alaA"
                     /locus_tag="OO568_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6639822.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            21062..21661
                     /gene="yfbR"
                     /locus_tag="OO568_000328"
     CDS             21062..21661
                     /gene="yfbR"
                     /locus_tag="OO568_000328"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6639823.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21720..23552)
                     /locus_tag="OO568_000329"
     CDS             complement(21720..23552)
                     /locus_tag="OO568_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6639824.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23639..24289)
                     /gene="hxpA"
                     /locus_tag="OO568_000330"
     CDS             complement(23639..24289)
                     /gene="hxpA"
                     /locus_tag="OO568_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR6639825.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(24300..24794)
                     /locus_tag="OO568_000331"
     CDS             complement(24300..24794)
                     /locus_tag="OO568_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6639826.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24877..25332)
                     /locus_tag="OO568_000332"
     CDS             complement(24877..25332)
                     /locus_tag="OO568_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6639827.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25670..26872
                     /gene="ackA"
                     /locus_tag="OO568_000333"
     CDS             25670..26872
                     /gene="ackA"
                     /locus_tag="OO568_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6639828.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26947..29091
                     /gene="pta"
                     /locus_tag="OO568_000334"
     CDS             26947..29091
                     /gene="pta"
                     /locus_tag="OO568_000334"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6639829.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            29281..30801
                     /gene="yfcC"
                     /locus_tag="OO568_000335"
     CDS             29281..30801
                     /gene="yfcC"
                     /locus_tag="OO568_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6639830.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30834..31376)
                     /gene="yfcD"
                     /locus_tag="OO568_000336"
     CDS             complement(30834..31376)
                     /gene="yfcD"
                     /locus_tag="OO568_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6639831.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(31434..31985)
                     /gene="yfcE"
                     /locus_tag="OO568_000337"
     CDS             complement(31434..31985)
                     /gene="yfcE"
                     /locus_tag="OO568_000337"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6639832.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(32041..32685)
                     /gene="yfcF"
                     /locus_tag="OO568_000338"
     CDS             complement(32041..32685)
                     /gene="yfcF"
                     /locus_tag="OO568_000338"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6639833.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32821..33468
                     /gene="yfcG"
                     /locus_tag="OO568_000339"
     CDS             32821..33468
                     /gene="yfcG"
                     /locus_tag="OO568_000339"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6639834.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33525..33887
                     /gene="folX"
                     /locus_tag="OO568_000340"
     CDS             33525..33887
                     /gene="folX"
                     /locus_tag="OO568_000340"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6639835.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIVREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33908..34801
                     /locus_tag="OO568_000341"
     CDS             33908..34801
                     /locus_tag="OO568_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6639836.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSHWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34849..35739)
                     /gene="rpnB"
                     /locus_tag="OO568_000342"
     CDS             complement(34849..35739)
                     /gene="rpnB"
                     /locus_tag="OO568_000342"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6639837.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35936..36709)
                     /gene="hisP"
                     /locus_tag="OO568_000343"
     CDS             complement(35936..36709)
                     /gene="hisP"
                     /locus_tag="OO568_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6639838.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36717..37433)
                     /locus_tag="OO568_000344"
     CDS             complement(36717..37433)
                     /locus_tag="OO568_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6639839.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(37430..38116)
                     /gene="hisQ"
                     /locus_tag="OO568_000345"
     CDS             complement(37430..38116)
                     /gene="hisQ"
                     /locus_tag="OO568_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6639840.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     LGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(38206..38988)
                     /gene="hisJ"
                     /locus_tag="OO568_000346"
     CDS             complement(38206..38988)
                     /gene="hisJ"
                     /locus_tag="OO568_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6639841.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(39209..39991)
                     /gene="argT"
                     /locus_tag="OO568_000347"
     CDS             complement(39209..39991)
                     /gene="argT"
                     /locus_tag="OO568_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6639842.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALLPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(40257..40826)
                     /gene="ubiX"
                     /locus_tag="OO568_000348"
     CDS             complement(40257..40826)
                     /gene="ubiX"
                     /locus_tag="OO568_000348"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6639843.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40921..42438)
                     /gene="purF"
                     /locus_tag="OO568_000349"
     CDS             complement(40921..42438)
                     /gene="purF"
                     /locus_tag="OO568_000349"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6639844.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(42475..42963)
                     /gene="cvpA"
                     /locus_tag="OO568_000350"
     CDS             complement(42475..42963)
                     /gene="cvpA"
                     /locus_tag="OO568_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6639845.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(43222..43884)
                     /gene="dedD"
                     /locus_tag="OO568_000351"
     CDS             complement(43222..43884)
                     /gene="dedD"
                     /locus_tag="OO568_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6639846.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43874..45142)
                     /gene="folC"
                     /locus_tag="OO568_000352"
     CDS             complement(43874..45142)
                     /gene="folC"
                     /locus_tag="OO568_000352"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6639847.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIELGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGRELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADAAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(45212..46126)
                     /gene="accD"
                     /locus_tag="OO568_000353"
     CDS             complement(45212..46126)
                     /gene="accD"
                     /locus_tag="OO568_000353"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6639848.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(46282..46941)
                     /locus_tag="OO568_000354"
     CDS             complement(46282..46941)
                     /locus_tag="OO568_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6639849.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(47024..47836)
                     /gene="truA"
                     /locus_tag="OO568_000355"
     CDS             complement(47024..47836)
                     /gene="truA"
                     /locus_tag="OO568_000355"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6639850.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47836..48849)
                     /locus_tag="OO568_000356"
     CDS             complement(47836..48849)
                     /locus_tag="OO568_000356"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6639851.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQLWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48915..50051)
                     /gene="pdxB"
                     /locus_tag="OO568_000357"
     CDS             complement(48915..50051)
                     /gene="pdxB"
                     /locus_tag="OO568_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6639852.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            50150..51145
                     /gene="flk"
                     /locus_tag="OO568_000358"
     CDS             50150..51145
                     /gene="flk"
                     /locus_tag="OO568_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6639853.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMPTRPALWILLVAIILMLVWLVR"
     gene            complement(51142..52320)
                     /locus_tag="OO568_000359"
     CDS             complement(51142..52320)
                     /locus_tag="OO568_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HCR6639854.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52595..53815)
                     /gene="fabB"
                     /locus_tag="OO568_000360"
     CDS             complement(52595..53815)
                     /gene="fabB"
                     /locus_tag="OO568_000360"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6639855.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDITGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53974..55980
                     /gene="mnmC"
                     /locus_tag="OO568_000361"
     CDS             53974..55980
                     /gene="mnmC"
                     /locus_tag="OO568_000361"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6639856.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAR"
     gene            complement(56101..56379)
                     /locus_tag="OO568_000362"
     CDS             complement(56101..56379)
                     /locus_tag="OO568_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6639857.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(56413..56961)
                     /gene="epmC"
                     /locus_tag="OO568_000363"
     CDS             complement(56413..56961)
                     /gene="epmC"
                     /locus_tag="OO568_000363"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6639858.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56961..57770)
                     /locus_tag="OO568_000364"
     CDS             complement(56961..57770)
                     /locus_tag="OO568_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6639859.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57770..58594)
                     /gene="mepA"
                     /locus_tag="OO568_000365"
     CDS             complement(57770..58594)
                     /gene="mepA"
                     /locus_tag="OO568_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6639860.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58598..59683)
                     /gene="aroC"
                     /locus_tag="OO568_000366"
     CDS             complement(58598..59683)
                     /gene="aroC"
                     /locus_tag="OO568_000366"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6639861.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKEGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59718..60650)
                     /gene="prmB"
                     /locus_tag="OO568_000367"
     CDS             complement(59718..60650)
                     /gene="prmB"
                     /locus_tag="OO568_000367"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6639862.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            60816..61367
                     /gene="smrB"
                     /locus_tag="OO568_000368"
     CDS             60816..61367
                     /gene="smrB"
                     /locus_tag="OO568_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6639863.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(61471..62361)
                     /locus_tag="OO568_000369"
     CDS             complement(61471..62361)
                     /locus_tag="OO568_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6639864.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(62348..62872)
                     /locus_tag="OO568_000370"
     CDS             complement(62348..62872)
                     /locus_tag="OO568_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6639865.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62869..63339)
                     /locus_tag="OO568_000371"
     CDS             complement(62869..63339)
                     /locus_tag="OO568_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6639866.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDIAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(63336..63884)
                     /locus_tag="OO568_000372"
     CDS             complement(63336..63884)
                     /locus_tag="OO568_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6639867.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63889..64611)
                     /locus_tag="OO568_000373"
                     /pseudo
     CDS             complement(63889..64611)
                     /locus_tag="OO568_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64631..65353)
                     /locus_tag="OO568_000374"
     CDS             complement(64631..65353)
                     /locus_tag="OO568_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6639868.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(65413..67272)
                     /locus_tag="OO568_000375"
     CDS             complement(65413..67272)
                     /locus_tag="OO568_000375"
                     /inference="COORDINATES: protein motif:HMM:NF011812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HCR6639869.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGRTAVRSVISAVSMTRLTIS"
     gene            complement(67354..67917)
                     /locus_tag="OO568_000376"
     CDS             complement(67354..67917)
                     /locus_tag="OO568_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6639870.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(68601..69086)
                     /gene="sixA"
                     /locus_tag="OO568_000377"
     CDS             complement(68601..69086)
                     /gene="sixA"
                     /locus_tag="OO568_000377"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6639871.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(69289..71433)
                     /gene="fadJ"
                     /locus_tag="OO568_000378"
     CDS             complement(69289..71433)
                     /gene="fadJ"
                     /locus_tag="OO568_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6639872.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(71433..72743)
                     /gene="fadI"
                     /locus_tag="OO568_000379"
     CDS             complement(71433..72743)
                     /gene="fadI"
                     /locus_tag="OO568_000379"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6639873.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72923..73207)
                     /locus_tag="OO568_000380"
     CDS             complement(72923..73207)
                     /locus_tag="OO568_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6639874.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            73579..74919
                     /gene="fadL"
                     /locus_tag="OO568_000381"
     CDS             73579..74919
                     /gene="fadL"
                     /locus_tag="OO568_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6639875.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLQADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            75285..75569
                     /locus_tag="OO568_000382"
     CDS             75285..75569
                     /locus_tag="OO568_000382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639876.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQ"
     gene            75662..76336
                     /locus_tag="OO568_000383"
     CDS             75662..76336
                     /locus_tag="OO568_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639877.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNCQAKYQNEINQLSALG
                     VNFDDI"
BASE COUNT        19317 a        20448 c        18739 g        17894 t
ORIGIN      
        1 aaatactggc ggacactgga tattagtttc gctacagaaa atagtaaata aaaaaaataa
       61 taaactacaa ataaaatgcg tcatattcaa ctcattgcgt gcactaggcc atgataaaga
      121 aaattcactt aagcgtgtca ttaatagttt taattctgaa ctcatgggag aaatgtcgaa
      181 taataatata aaagttcatt taaatgaacc agagataata tttttacatg ccgatcttca
      241 gcaatactta agccaaagtt gcggtgcgtt tgtgtgcatg gcagcccagg aagtgattga
      301 acaaagggaa agcaattctg acagcgcccc ctatacgtta ttaaaaaact atgctgacag
      361 atttaaaaaa tattcagcag aagagcagta cgaaattgat tttcaacatc gactggtaaa
      421 cagaaattgt tatttagata aatatggcga tgcacgaatc aatgcttctt atacacaatt
      481 agaaataaaa cactcacacc ccaaaaatag agcatccggc aaaagagtga gttaacatca
      541 ttgttaatca attcgaccag tcagtaaata tgctgactgg tcgaacataa tttgctaaaa
      601 agtcatcact gactgatgtc agtcccacca tctgccagtt caatctacgg gtatttccag
      661 cgtatcgcgg cacgcgattg ctggtctgtt gatgtagtgg attgtagttg ctgacttttc
      721 ttaccctcag atttctgccc cacttgtcaa ttcaatctgc caataaataa cataattata
      781 tttttattcc tcacagaaat tcctttttaa aaatagcgaa cccactgtaa aaaacacttt
      841 ttgtatacct ctaccaagcg taataaaata aacgctcata attcattata aataatgatg
      901 aaataacgcc ttttattgat aatttacata tgaaaaaatt tttccttttg ttatattatt
      961 tctgttttca ctccctctga ttatttttac caaccctggg tcactgcatt atcaccaacg
     1021 ccccgacacg taagtcctga gcaattagaa aaaacagttc gttatcttac acaaactgtg
     1081 catccacgta atgccggcaa tattgataat ctgaataggt ccgcagagta tataaaagag
     1141 ttctttgtca gtagcggtgc cagagttacc gcgcagggcg tccccgattg gctattacga
     1201 ctctgcgcct ggaagccaga attaccctta cccggcaatg tcctggcttt atcccgatcg
     1261 gggagacttt attgctgtgg tcggcagaat gcaggatatc aacgccgttc gtcaggtaaa
     1321 agcggcattg ttgtcatccc gggatttatc tgtttattct atgaatgccc cagggtttat
     1381 tcccggtatt gatttttctg atcaccttaa ttattggcaa cacgatattc ccgccgtaat
     1441 gattactgac accgcttttt atcgtaataa acaataccac ttgcccggtg ataccgcaga
     1501 cagattgaat tatcagaaaa tggctcaggt ggtagatggt gttataacct tgttatacaa
     1561 cagtaaataa taaaaatttc tcgaggcggg tccagaaaca gcgacatgta cattttattt
     1621 tccctcgtct gttttaagat gcgaaacata aaattcaatg gaggaaaaat aaatgggaat
     1681 gattggctat tttgcagaaa ttgactcaga aaagattaac caactgcttg agtctactga
     1741 aaaacctttg atggataata tacatgacac gctttcagga ttacgccgac tggatattga
     1801 taaacgctgg gattttttac actttgggct gacaggcacc tctgcttttg atcccgcgaa
     1861 gaatgatccg ctgagtcgtg ccgtgcttga tgaacacagc ctcgaagatg gcatagatgg
     1921 cttcctggga ttgacctgga atcaggaatt agccgcgaca atcgaccgac tggaatcact
     1981 ggatcgaagt gagctgcgta aacagttttc cattaagcga ctcaatgaaa tggagattta
     2041 tccgggagtg accttttcag aagagttaga ggggcaactc tttgcctcga tcatgcttga
     2101 tatggaaaag ctgattagcg cgtatcgacg tatgctgcga caagggaatc atgcgctgac
     2161 ggttattgtt gggtgatggt gtcagagcgc caaaaaaaac cgccgaacat gtcggcggct
     2221 ttctgactta caaactaaca gtttacatca gcggcattgc taaccgcaca atactaatca
     2281 gcggttgtgg ccatacaccc agcaccagta ccaacagtgc agaaatcagc accacgatac
     2341 cgcccgcgct gtactgccag tttgacggtg catcgcgacc cggttgttcc ggtgcgtgca
     2401 ggtacaggct caccgccacg cgcaggtagt agtagaggcc gattgccgaa ccgacaacca
     2461 cggcacccac cagccaccac aagtgtgcct ggacaccgac cgccagcacg tagaacttac
     2521 cgataaagcc cagcgtcatc gggataccgg ccagagacag catcatcacc gtcatcactg
     2581 ccgcgaggat cggacgatgc cagaacagac cgcggtaaga gaacagggaa tcagcatccg
     2641 ggccacgata cgggctggac atcaggctga ccacgccgaa cgcgccgagg ctgctgaaca
     2701 gataaccggc caggtaaacc cctaccgctt ccatcgacat ctcgccggtt tgcagcgcaa
     2761 tcagcgccac cagcagatag ccgaggtgag agatagatga gtaaccgagc agacgtttga
     2821 tgttggtctg gctcaacgcc atcaggttac cgaagatgat ggaggcaaag gcgataatcg
     2881 ccagtactac gcgaatcgct tcgctgtcac ccaccggtgc gtagaggaac agacgcatca
     2941 ccacaccgaa gatagcgatt ttgctcgccg tcgccaggaa agtggaaacc ggcgcaggcg
     3001 cgccctggta tacgtctggc gtccacaggt ggaacggcac cagagagagt ttgaagccga
     3061 ggccaacaat catcatgccg aaacctgcca gcaacagcgg ctcgttgagc ataccgtcgc
     3121 cgaggttttt acccagcgcg acaaacgaca ggtcgccaga ctgcgcatac accagcgcca
     3181 taccgaacag caggaaagaa gacgctgcgg cagaaaggat ggtgtatttg atactggctt
     3241 ccagcgaacg tttctggcgg aaagcgtaac cgaccaggcc aaacagcggc aaagagatca
     3301 gttcaatacc gaggaacaga gacgccagat ggtttgcatt cgccagcagg atcccgccca
     3361 gcgcggcaat taacaccaac aggtagaact catccttgtt gtcgttatag ccttcaagcc
     3421 acgggtaggc gaaagtacag gtggcgaggc tcgccaacaa taccagcccg gtgtaaagca
     3481 tggcgaaacc atcaacgcgc atcagcggcg taacgtccat agcgcccgcc tggccaacaa
     3541 accagagcga aaccagcgcc gcgttaagcc caataaccga gagcgtagcg ttgaggaaat
     3601 gattgcgtcg ccacgcaatg gagagcatca caaccaccac cgtcaagccg acgatcagca
     3661 acggtagcag tgcgatcagg ttttgtggag ttattgtcat ggcgatttac ggccttgtag
     3721 tagtaacgga attaacaaac cactgctgga tattgccaat cgcggagtgc gaggtatcca
     3781 gaatcggctg cggatagaag cccagcagta ccagcagcac caccagcaac aggatcataa
     3841 acagctcacg cagcgacatc cctggcagtt cctggctggc aatctggctt ttcgctttac
     3901 cgaagtaagc gcgatgcaac atcgccagcg aataaacaga tgcaaagacc agcccaaagg
     3961 tagagataac ggtaatcacc gggacaacct ggaagctgcc gaacagaatc ataaattcgc
     4021 cgacgaagtt accggtgcca ggcatcccaa gcgttgccac cgcaaagaac agcgacagtg
     4081 ctggcagcca tttcatcttg ctccacagac cgcccatcat gcgcatgtcg cgggtatgga
     4141 tacgttcata aagctgaccg cagagaataa acagacccgc cgccgacaag ccgtgcgcaa
     4201 tcatctggat taccgcgccc tggtaggcca actggctgcc ggtgtagatt gcaatcagca
     4261 cgaagcccat gtgggaaacc gaggtgtagg cgatcagacg tttgatatcg gtctgagcga
     4321 aggccatcca cgcaccgtag aagatgccga taacacccag ccacatagcg attggcgcga
     4381 actctgccga cgcgttcggg aacagcggca gggagaaacg caacaaaccg taagcggcgg
     4441 ttttcagcaa gatccccgcg aggtccacgg aaccggcggt cggagcctgg gagtgcgcat
     4501 ccggcagcca gccatgcagc ggaaccaccg gcattttgac tgcgaaggcg atgaagaagc
     4561 ccagcatcaa cagatattcc acaccactgg acattggcgt attcagcagc tcttcatagt
     4621 tgaaggtcca gacgccggtc gcattgtagt gaacaaaaac cagcgccagg atggcgatca
     4681 acatcaccag accactcgcc tgggtgtaaa tgaagaactt ggttgccacc gtgatacgcg
     4741 ttttaccgtc agaggcttta tgcccccaca gtgcgatcag gaagtacatc ggcaccagca
     4801 tcatttccca gaagaagaag aacaggaaca tgtcgatggc aaggaacacg ccgataacgc
     4861 cgcccaggat ccacatcagg ttgaggtgga agaagccctg atatttttcg atctctttcc
     4921 acgaacatag taccgccagc acaccgagca gaccggtcag cacgaccatc agcagcgaca
     4981 gaccgtcaat ggcgagatga atagagatac caaaacgcgg gatccacggc atgtcgaatt
     5041 cagactgcca ctgcggaatt ccggcggatt gcgtcagtga ataaccgccc tgcaaccaca
     5101 gttgcagcga cagcgccagc gtcaatccca tggtgatcag cgcgatccag cgtggcacct
     5161 tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc aataaaggga attaatatta
     5221 gccagggtag taacatggcg atctttattc cttgtaaaag tcccgtcagg accggatttt
     5281 caacgaattc tcacgacaga aaattctctt cggactgggg gtagtgtcgg atgcgccgcg
     5341 aaggcagctt atccgaccta cgttcttata tgcccagggg caatcccaca atccttaact
     5401 caacgcagta ccatcaacag tgccagcacc acgaccgcac cgatgctcat ggatgccaca
     5461 taccagcgca gataaccgtt ctcgcttaac agcagacctt tacctgcaaa gcgggaaaga
     5521 accgccggga tgttcatcat tgagttcagc ggatcgcgtt tcagcaacca ggcaataccc
     5581 aggaacggct tgacgaacac tttgtcgtac agccagtcaa agccccaggc gttgtaccac
     5641 caggtactca gcaggcggcc cggcgcactg ttggcgatgg aggtcaccag agtacgttta
     5701 cccagccaca gccaggctgc cagcagaatg ccgaccaccg cgaccacgcc agaggtaatt
     5761 tccagggtca acatgctgcc gtgcgccagt tccgtcgttt gcggaagcac gccctgcagc
     5821 ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca gcagcacaat cagcggcagg
     5881 ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt cttttccgtg gaagacgatg
     5941 aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac cgaccagacc tgccaccatc
     6001 agattgatat gaccattcgc catcgcaccc gcgaggatct catccttact gaagaagccc
     6061 gcagtgacca gcggcagtgc cgacagtgct gcgccgccca ccaggaagca gagataaacc
     6121 agcggaatag atttacgcag accgcccatc ttgaagatgt tctgttcgtg atggcaggcc
     6181 agaatgacgg aaccggatgc caggaacagc agcgctttaa agaacgcgtg agtcatcaag
     6241 tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga ggaacatgta gccaatctgg
     6301 ctcatggtag agtaagcgag aacacgtttg atgtcggtct gtaccagcgc ggcaaaaccg
     6361 gccagcagca gcgtaaccgc cccgacaata cccaccagat gcagaacttc cggcgtcatc
     6421 aggaacaggc cgtgggtacg ggcgatcagg tagacacccg cggttaccat cgttgcggcg
     6481 tggatcagcg cggagacagg cgtcgggccc gccatcgcgt cagcaagcca tgtctgcaac
     6541 ggcaactgcg cagatttacc gaccgcaccg cccagcagca tcagcgtcgc ccacatcagc
     6601 atgttattgc cgtcagcaaa gtgcgctggt gccagttcca ccatttcgcg gaagttcagg
     6661 gtgcccagtt cgttgtaaag aatgaacagt gcgaaagcga ggaaaacgtc acccacacgg
     6721 gtcacgacga acgctttcat tgctgctgcg ccattcttcg gatcggtgta atagaaaccg
     6781 atcagcagat aggagcacag gcccacgcct tcccagccga ggtacatcag cagcaggtta
     6841 tcggcgagaa ccagaactac catgctggcg atgaacaggt tggtgtaggc gaagaagcga
     6901 gagtagccct cttcaccgcg catgtaccag gaggcgtaca tgtgaataag gaaacccaca
     6961 ccagtgacca ccgagagcat ggtcagcgac aggccgtcca gcaccaggtt aaaaccgatg
     7021 ttaaagtcgc ctaccgacat ccacgtccac agcggctggc tgtatgcctg ctcgccgtta
     7081 gcgaagaaat caacgccgat aaaggcggtt accagcgccg ccaggcccac agagccaacg
     7141 ccgacgatcg ccgaaacgtt ttcagaccag cgcccacggg agaacgccag caggacgaag
     7201 ccaatcaatg gcaaaataat ggttaaggca agcatgttca tccgcgcatc tcacttactg
     7261 aatcgatgtt caggttctgg cgacgacggt gaagttgcag cagcagcgca aggccgatac
     7321 tcgcttctgc cgccgcgagg ctgatggcga gaatgtacat cacctgaccg tcggtctgac
     7381 cccagtagct tccggcgacc acaaaggcca gcgcggaggc gttaatcatg atttccagac
     7441 caatcagcat aaacagcaga ttgcgacgga taaccagacc ggttaagcca agaacgaata
     7501 agattgccgc gaggatcagt ccatgttgta aggggatcat gcgtgctcct ccgtttttct
     7561 tttcgcgctg tcgtctttac gattgctcag cacttcaccc gcacgctctt cacgaccgac
     7621 gtggaaggcc acaaccagac ccgcgagcag cagcatagaa gccagttcca ccgccagtac
     7681 gtaaggcccg aacagcgtaa tacccactgc tttagcactg attggcgtac cgtcgatacc
     7741 ctgatcgtta acaccgagga tggcgtaaac aatcaccacc agcatgatgg ccgacaaaat
     7801 tgccgggcca atccacacct gcggtttcag ccactggcgt tcctgttcga tttctgaacc
     7861 gcccaggttg agcatcatca ccacgaacac gaacagcacc ataatggcac ccgcgtagac
     7921 gataatttcc agcgcaccgg cgaagtaagc gcctagtgag aagaacaccc cggaaatcgc
     7981 cagcagcgaa ataatcaggt acagcagtgc gtgtaccgga ttggtatggg tgatcactcg
     8041 caaggtcgca agtatggcta tcaggccaca gatataaaaa gcgaactcca ttgcccctct
     8101 ccttacggta acaggctctt gacgtcgata ggcttggctt cgttctctgc ttcgccctta
     8161 tctttgccgt cgattgccat acctgccatc cggtagaagt tatattccgg gtatttgccc
     8221 ggaccggaga tcagcagatc ctctttctcg taaaccagat cctggcgctt gtattccccc
     8281 atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac aggcttcttc gcacagacca
     8341 cagaaaatgc agcgtgagaa gttaatgcgg aaaaattccg gataccagcg accgtctttg
     8401 gtttctgctt tttgcagcga gatacagccg accgggcagg ctaccgcaca gaggttacag
     8461 gctacgcaac gctcttcgcc gtccgggtcg cgggtcagaa cgatacgacc acgataacgg
     8521 ggcggcagat agaccggctc ttccgggtac attcgcgttt cgcgtttggc gaacgcgtgc
     8581 aggccgatca tccagatact acgaacctgg gtgccgaaac ctactaacaa ttcttttaag
     8641 gtcatggtct tattgcccct tattgcgcct gccagagaat gacagccgcc gttaccagca
     8701 agttgatcag cgtcagcggc aggcagattt tccagccgaa ggacattacc tggtcataac
     8761 gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat cataaagaac gcggttttca
     8821 gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca gccaccgaag aacagcgtca
     8881 ccatcaatgc agagatggtc acaatcccga tgtactcacc cacgaagaac agaccgaact
     8941 tcataccgga atattcaatg tggtaaccat ccgccagttc ctgctcggct tccggctggt
     9001 caaacgggtg acggtgacac accgccacgc ccgcgatggc aaaggtaata aaaccaaaga
     9061 attgcgggat gacgttccac acatgcgcct ggctgttgac gatgtcggtc atgttgaatg
     9121 aaccggcctg cgccaccacg cccatcaggg aaagcccgag gaacacttcg tagctcaggg
     9181 tctgcgcaga agcacgcatc gcacccagca gcgagtattt gttgttactt gaccagcccg
     9241 caaacagcac cgcgtaaacc gccagacctg ccatcatcag gaagaacaaa atcccgatgt
     9301 tcaggtcggc aaccacccaa cccggactga ctggcacaat tgcaaaggcc agcagcagcg
     9361 aggtaaaggc aatcatcggt gccagggtaa agatgacgcg atccgagaat ttcgggatcc
     9421 agtcttcttt aaagaacatt ttgatcatgt ccgcaaccag ctggagcgaa ccgccccagc
     9481 caacacggtt aggtccataa cggttctgga acagacccag caggcgacgt tcgccaaagc
     9541 tcatgaatgc cccacaggtg acaaccacca gcaggatccc caccgctttg aggatggtca
     9601 gcaggatctc aatcagttcc ggtgatatcc aactcattgt tgtgcctcct tgagatcctc
     9661 aagatgcgcg ccagccagca ccggagcaat gccggacata cccatcggca agcccacctg
     9721 ccctgccgtc agtccttcgg cgatttcaac cggcagcgtg accgtgttgc catcgtaact
     9781 aaaggagacg cgtgtacctg cgttcacacc caacttcgcg gcatccgctg ggttgagttt
     9841 gatgtacggc tgcggcatac ggctctggaa gaccggagca cgctgtgaca attcatcgct
     9901 gccaaacagg tggtagtacg gcgcgatacg ccatttcccg tcctgcggct ggaagcgtgc
     9961 cggtacgctg gtgaagtaat ccagaccgtt ttcgctggtt tcaaacagac gcacgcccgg
    10021 atcgccaaag cgcagtttgc cgcccacttc gtcctggaat ttgttccacg cctgcgggga
    10081 gttccagccc ggcgcccagg caaacggcac ttgcgaacgg tgcgcggtcg gctggttgtt
    10141 accttccatc gagaaggtga acatggtgtc aatatcctgc ggctgacgcg gctcatgaac
    10201 gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg tgcggttcac gggccagttt
    10261 ctgcccacga atacggaatg tcgcatccgg cgcagcatct ttgatacccg ccagttccgg
    10321 gattttcgcc acaacagcgt caattacatg gtcgagctgc gtccagtcca cttcacggct
    10381 cagcaaggtg ctgtgcagcg agtgcagcca gcgccagctt tccagcatga cagttttgct
    10441 gtcgtaataa gccggatcgt aaacctggaa gaaacgttgg gcgcgacctt cattattaat
    10501 taccgtaccg tcgctttcag caaagctggc agcggaaagc accagatggg cgttttccat
    10561 gatcgctgtg cgttgatgat caaccaccat caccagcggc gctttagcca gcgcagcatt
    10621 cacgcgggta gcagaagcgt aacgatgcag atcgttttcc aacaccacca ccgcgtcggc
    10681 gcgtccggtt tccagttcgg ttaacgcttc ttcaagcgaa ccgccaccca taatgcccag
    10741 ccccatgctg ttgacggaac gggcaatcat ggtgatacca acgtcagcgc cgcgaccttt
    10801 cagggctttc gcgacgttag ccgcagcctg aatcacctct atgctaccgg cgttcgtccc
    10861 ggagataatc aacggtttct tcgcacctgc cagtgcctgc acgatgacgt cgattttgtt
    10921 ttgcagctca ggttcgatac cgtcaaccgc tggtgcggag ttatccagcg catgggcgat
    10981 ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg taagtccacg ccgcgatatc
    11041 atccagacga gtgtcatcaa cgttggtaac aaacagcgga tgcttcgcac gttgaccgat
    11101 gttgaggatt gccgcaatct gccagtcagc cactttctgt gctgcagcca tttcgcgcgc
    11161 tttacctttc accgcctgac gcactgccag tgcgacgcgc gcgccggtct gggtaacgtc
    11221 ttcgcccagc accagtaccg catcgtaaga ttcgatttcg cgcagagccg gagtataaat
    11281 gccgccttca cgcagcactt tcagcgccag ttgcagacgt tcctgctcac cgtgagcgat
    11341 accggtgtag aagttttctt cgcccaccag ttcacgcagc gcaaagttgc tttccacgct
    11401 ggcacgcgga gaaccaatac cgatcacttt cttcgactga cgcagaatat ctgccgcgcc
    11461 ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg ccacgacgct gtactggctg
    11521 acgcggacga tccttcaggt tgacgtaacc gtaaccgaaa cgaccacggt cgcagaggaa
    11581 gtagtggttt accgtaccgt tgtaacggtt ttcgatacga cgcagttcgc cgtaacgttc
    11641 accggggctg atgttacagc cgatggaaca ttgctggcag atgctcggcg caaactgcat
    11701 atcccattta cggttgtaac gctcggagtg cgttttgtcg gtaaatacgc cggtcgggca
    11761 aatttcgacc aggttaccgg aaaattcgct ttccagcgtg ccgtcttccg ggcgaccgaa
    11821 gtagacgttg tcgtgcgcgc cataaacgcc cagatctgta ccgtcagcgt aatctttgta
    11881 gtaacgcaca cagcggtagc aggcgatgca gcggttcatt tcgtgagaga tgaatggccc
    11941 caaatcctga ttacggtggg tacgtttggt gaaacggtag cgacggaagc tgtgtccggt
    12001 catcacggtc atatcctgaa gatggcagtt accgccttct tcacataccg gacagtcgtg
    12061 cgggtggttg gtcattaacc actcgaccac actttcacgg aactgtttcg cttcttcgtc
    12121 gtcaatggaa ataaaggtgc catcggaagc cggtgtcata caggacatca ccaggcgacc
    12181 acgcgtgtct tccgcgtttt ggtattgctt caccgcacac tggcggcaag caccgacgct
    12241 tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg cccagagaca gacaagcttc
    12301 cagcaggttg tccgctccgt tgacctcgta ttctttgccg tctacatgaa ttgtagccat
    12361 tagcatgctt ccagttttct cggtcaggga ccgagcgtta atcgaaattc ggttaccagc
    12421 gctctttcag caggttcggc tgaatcccat taatcaaatg ggtattgctg aacggctgtt
    12481 tgattcccgc ctcaaattct tcgcggaaat atttgatggc gctctgtaac ggctccactg
    12541 caccaggtgc gtgggcacag aaagttttac ccgggcctaa gaatcgacac agttgctcaa
    12601 gtgtttcgat atcgcccggc tggccttcgc cacgctccag cgcacgcaga attttcacgc
    12661 tccacggcag accgtcgcgg cacggcgtac accagccgca ggactcacgg gcgaaaaact
    12721 cttccaggtt acgcaccagc gacaccatgt tgatctcatg gtcaaccgcc atcgccagcg
    12781 ccgtacccag acggctgccc gctttaccga tactttcgaa ttccatcggc agatcaaggt
    12841 gcgcttcggt caggaagtca gtccccgcgc cgcctggctg ccaggcttta aatttcagac
    12901 catcacgcat accaccggcg taatcttcga ggatctcgcg tgcggtggtg ccgaacggca
    12961 gttcccacag tcccggattt ttcacccgac cggagaagcc catcagcttg gtgccagcat
    13021 ctttactttt cgagatgttc tgataccact ccacgccgtt agcgaggatc gccggaacgt
    13081 tacacagggt ttcgacgttg ttgacacagg tcggtttgcc ccatacgccg gaggttgccg
    13141 ggaagggggg tttcgagcgt gggttagcac gacgcccttc cagggagttg attaacgctg
    13201 tttcttcccc gcagatgtag cgccctgccc cggtatggac gaacagttcg aagtcgaaac
    13261 ctgttcccat aatgtttttg ccaagcaagc ccgcttcagt ggcttcggca atagcgcggc
    13321 gcagatttac tgccgcttcg atatattcgc cacgcaggaa gatgtagcca cggtaagctt
    13381 tcagcgcaaa cgcggagatg agcatacctt ccaccagcag gtgcggcagt tgctccatca
    13441 acaggcggtc tttataggtg cccggctcca tttcatcggc gttacacagc aggtaacgga
    13501 tgttcatgga ttcgtctttc ggcatcaggc tccacttcag gccagtcgaa aagcccgcgc
    13561 cgccgcgccc tttcagacca gcgtctttta cctgattaac gatttcgtcc ggagacagcc
    13621 cggtcagcgc cttacgcgcc ccttcgtaac cgtttttgct gcggtattcg tccagccaca
    13681 ctggctgttt gtcatcgcgc agacgccagg tcagcggatg cgtttcggga gtacggataa
    13741 tgtttttcat ttataccgct ccagcagttc agggatcgct tccggggtca gatgcgcgtg
    13801 agtgtcctca tcgatcatca tgtttggccc tttatcacag ttccccaggc agcaagttgg
    13861 cagcagcgta aagcggccat caaatgtcgt ttgccctggt ttgatgttca gctttttctc
    13921 gagcgccgcc tgaataccct gatacccgtt gatatgacag accacgctgt cgcaataacg
    13981 gatcacatgg cgaccaaccg gctggcggaa gatctgactg tagaacgttg ccacaccttc
    14041 gacgtcgctt gccggaatac ccagcacatc ggcgatcgcg tggatcgcac catccggcac
    14101 ccagccacgc tgcttctgaa cgattttcag cgcttcaatg gacgccgcac gcgggtcttc
    14161 gtagtggtgc atctcgtgct caatcgcttc acgctctgcc gcactcagct caaaagcctc
    14221 ggtttgtggt tgttgattct cgtgcataat tagcggtcca catctgacat aacaaaatcg
    14281 atactgccca gataaacaat caggtcagac accaagctgc cgcggatcgc cgccggaatt
    14341 tgctgcaaat gcgcataact cggcgtgcgg atacgagtgc gataactcat ggtgctaccg
    14401 tcgctggtca ggtagtaact gttgatcccc ttggtcgcct caatcatctg gaaagattca
    14461 ttggcaggca tcaccggccc ccacgacact tgcaggaagt gggtgatcag ggtttcgata
    14521 tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg ggtgatccgc tttgaacggg
    14581 ccttccggca tgttgttgag gcactgctca agaatgcgca gactctggcg cagctcttcc
    14641 actttcagca ttacgcgggt gtagcagtca gaaacaccac caccaaccgg gatttcaaag
    14701 tcgaagtttt catagccaga ataaggacgc gccttacgca cgtcgaagtc gatcccggta
    14761 gcacgcaggc ccgcgccagt ggtgccccac tccagcgcct ctttcgcgcc ataggcggca
    14821 acgccctggg tacgaccttt cagaatggta ttttgcagcg ccgctttctc gtaagacgcc
    14881 agacgtttcg gcatccagtc gaggaactca cgcagcagac gatcccagcc gcgcggcagg
    14941 tcgtgcgcta cgccgccaat acggaaccac gccgggtgca tacggaaacc cgtgatcgct
    15001 tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga agaacacggg cgtcattgcg
    15061 ccgacgtcct gaataaaggt cgagatgtac agcaggtggc tgttgatacg gaacagttcg
    15121 gagagcataa cgcgaatgac gttaacgcga tccggcacgg tgatcccggc cagtttctct
    15181 accgccagca cgtaaggcat ttcgttaacg cagccgccga ggtattcaat acggtcggta
    15241 tacggaatgt agctgtgcca ggactggcgc tcgcccattt tctccgcacc acggtggtgg
    15301 tagccgatgt ctggtacgca gtcgacaatc tcttcgccgt cgagttgtaa aacgatacgg
    15361 aaagccccgt gcgccgacgg gtggttcgga ccgaggttga ggaacatgaa gtcctcgttt
    15421 tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg tcagggcttc catctccaga
    15481 tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg tagcgcgcgc cgggtaatct
    15541 ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga tgcggcgcag gttcgggtga
    15601 ccgtcgaaag taatgccaaa cagatcccag gtttcacgct cataccagtt agcgttcggg
    15661 aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg ccagcgccac cttcagcatg
    15721 atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa cggaaaaatc cgcggcaggt
    15781 aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt gtaagtcaaa cagcatgacg
    15841 taaggtttcg gcagtttctt taagaaatcg ccaacttcca gtaattgttc acgcttgatc
    15901 cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa aggcatccgg cccaaaacgg
    15961 ttgcgcagtt cgccaattac cggatcatca agatgatcgc gggtctgcca ggcgggttct
    16021 tgcgcggtta agtcggtcat attgttcacc attgcaaatg gtccgtggtg actgtcggcg
    16081 cagtaagctt cgcgaaatag atttaggaat aagcgaagaa aaatcccttt gccgacaggc
    16141 gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg caatgcgttc accgcgcttg
    16201 cgttcgcgct ctgattgcat attggcgcga taaacgccct gatcgccaac cacccaggag
    16261 agcggacgac gttctttgcc gatagattcc tgcaacagca tcagtgcctg catgtacgct
    16321 tcaggacgcg gcgggcagcc cgggatatac acatcaaccg ggatgaattt atcgacgccc
    16381 tgcacaacgg aataaatatc gtacatacca ccagagttgg cacaggcccc cattgagata
    16441 acccattttg gttccagcat ctggtcatac agacgctgaa taaccggtgc cattttggta
    16501 aagcaggttc ctgcaaccac catcaggtca gcctgacgcg gcgaagcacg caatacttct
    16561 gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag tcaccatctc aacgtaacag
    16621 caggaaagac cgaagttata cggccaaatt gagtttttac gaccccagtt aaccatgtca
    16681 ttgagcttgc ccataaacac gtttttgtta acttcttgct ccagagggtc ggttacgatc
    16741 tcctgctttt gcagggggta acggtcgttc tcaccgttgg gatctatgcg ggtgagcgta
    16801 taatccatct taatgcctcg cggttagcgt tgacgattag cgatactgtt cgtttccggg
    16861 ttcatacact cgcggcgtga acgcgcgggc gtccagtcca gcgcgccaat acgcaccaga
    16921 taaaccagac ctgccagtaa cacaaaaata aaaattgcag cttccacaaa gcctacccag
    16981 ccgctttcgc ggatagaggt tgaccatgcg aacagataca gcgcttcaac gtcgaagata
    17041 acgaagaaca tggccaccag ataaaacttg gcggacaggc gtaagcgggc ggagccgacc
    17101 gaatcgatac cggattcaaa cggcacgttt ttcgacctcg cgcgtgcgcg accgcctaaa
    17161 aaccaaccgc ctaccagcat caggcaacac aggccaatgg caacgataag aaagatagcg
    17221 aatgcccagt gatgagcgat gacttcagtg gatgttgaca tactcattgc ttactcatca
    17281 aaagtagcgc cagattcact gctcttcacg gcagatggac gccacatcga ttcatgggga
    17341 ggaataaaaa aaaccttaca atcactgtag aaattctttt atacagctaa ttgatgtggt
    17401 cttttactcc tttctataac cttttgtcaa ctttaacaaa agtttcttca cattagttta
    17461 cataatatca acaccattag catttaatgc cctttcaccc cagatccttg acgactccag
    17521 gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa tggaaaaaat acgggtctat
    17581 tttgacagga atttgtgtcg attcctcccc ccaaaagaga gtattttctt gatctgtgac
    17641 acgcttttgt tattccataa caaaaacgca gcaacaaatt tacgtatttt ttaacatcat
    17701 tgtagcaggt gatttttttc aggcgattat ttgtgcgttc gggacgtgaa tctctggtga
    17761 ttgaaaaata aacagttttg tacgttctgc actatgcgag gaaggatttt tactaaaaaa
    17821 aagccgctgg ggtttaaaac acccccagcg gctcgttttt tacactattg tctcaggaat
    17881 tatctatcgt ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc atccccttcc
    17941 ggagcagaca ttgggctgta ttgccacgga ttatggtagc tctccatcgc ctgatagatc
    18001 acctgggcca gctcattatt actggacggg tcatagcaca gcaaatattc ggtatcaggt
    18061 agcggcggta agccatctac tccactcagc acacgcaggt ccgggctcat catctcaacc
    18121 ggccttgccg tcacgccaag accggctttc actgccgcac gaacggccgg aagcgtcgag
    18181 gcgacataag ccagtcgcca tggaatatct gctttattaa gcgtcgccag caccatatcg
    18241 cgaaacggac ttggatcatc cagcaataca agagggatcg gctcgccttt ttgcaatacg
    18301 tattccgctg cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc cttaaacgct
    18361 gaaggacgat gggtggttac catcaaatcc acttcctggg attccagcat ttctgccata
    18421 taggcattac gtttaacgcg gacatccagc gctaatttcg gataaaccga actcacgcga
    18481 tttaacagga aaggtaagat cgtatcggca gattcatctg aagcaccgat agttaacacg
    18541 ccctgaagat tactgaacat taatgatgag caggcctcat cattaaaacg caggattttc
    18601 ctggcgtaac caagaagttg aatgccatgt tcagttaaca gtttgttgcg accgtgacga
    18661 gcgaacagtt ctttcccaac gagttgttcc agacgctgca tttgctgact tacggcggac
    18721 tgagtacgac acacagcggc agctgcggca gcaaaagtgt tcagatcggc aacagcaaca
    18781 aatgttctca gcagatcgag gtcgaggtta attatcggac gatttgcact tatcatatat
    18841 tatcacttac tggcggctca tactgagctg gttaatgctg tgcacacaca aacaagcaat
    18901 tccatttgta atgtgcctcc ctggcagttt catccggaac ccggacgaaa gtaaaaatgc
    18961 atatgagttg cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg aagtagatca
    19021 cagaatatat cttcagggat cgcatatcta ttaagttact cactcttttc tatttatgac
    19081 atgcgcgtgt ttgtataaat gtaaatgtga gtccttgttc cactctcgtg cagcatcgct
    19141 ggtcatacgc gaacacgtac aaacagcaat ggtgaagcgg catcaagagc agggatccgt
    19201 catttatgcg agcattttac cccaaaaacc ttttatttat aagggtcatt gcgaattatc
    19261 tgatgcaaag ttatgttatg ttatgttagg caaagtaatc ttctattatt aataagcaca
    19321 tcaaaacctt tttgaatatt aaataataat taattagcat catcctcatt cattaattcc
    19381 gcttaacaat agtttcacac cttaccccca ttgcgacacc tcggaaatca tcacgctgtt
    19441 aaactctccg taatgcggaa atatcatcat cgcgtttcat tagtgaattc ttaaatgagg
    19501 cattttcaca caattatctt acagataaaa aaaccatact tgcaattaag aattagaaca
    19561 attcactata taacattaca tgtaaagcat atacacctaa ttattttgtc attattaagt
    19621 tattaacagc acaatcgagc cttcccctct ggcaaaatct tattctgcag accttcaaaa
    19681 caccgtcctg ggggagtaca ttgttctaag ctgacttcca cggcagggag tggcgataac
    19741 agcaaaaaag gtcaagattc atgtccccca ttgaaaaatc cagcaaatta gagaatgtct
    19801 gttatgacat ccgtggtccg gtgctgaaag aagcaaaacg cctggaagaa gaaggtaaca
    19861 aggtactgaa actgaacatc ggcaacccag ccccattcgg ttttgacgcg ccagatgaaa
    19921 tcctcgttga cgtgatacgc aacctgccta ccgctcaagg gtattgcgat tccaaagggc
    19981 tttactccgc gcgtaaagcc atcatgcagc actaccaggc tcgtggcatg cgtgatgtta
    20041 ccgtggaaga tatttacatc ggcaatggtg tatcggagct tatcgttcag gcaatgcagg
    20101 cattgctgaa cagcggggac gaaatgttgg ttcctgcacc agattaccca ctctggaccg
    20161 cggcggtttc gctttccagc ggtaaagcgg tgcattatct ttgcgatgaa tcctctgact
    20221 ggttcccgga cctcgatgat attcgcgcta aaattacgcc tcgtacgcgt gggattgtta
    20281 ttatcaaccc aaataaccca accggcgcgg tatattccaa agagctttta atggagattg
    20341 tggagattgc acgtcagcat aatctcatta tcttcgccga tgaaatttat gacaaaattc
    20401 tctacgacga tgctgagcat cactcaattg cgccgctggc acctgacctg ctgaccatta
    20461 cctttaacgg actgtcgaaa acgtaccgcg ttgcaggctt ccgtcagggg tggatggtgt
    20521 tgaacgggcc gaaaaaacac gccaaaggct acatcgaagg tctggaaatg ctggcttcaa
    20581 tgcgcctgtg tgctaacgtt cctgcgcaac acgccattca gaccgcgctg ggtggttatc
    20641 agagcatcag tgaatttatt acccctggcg gtcgtcttta tgagcagcgt aaccgcgcgt
    20701 gggaactgat caacgatatt ccgggcgttt cctgcgtgaa acctcgtggt gcgctgtata
    20761 tgttcccgaa aatcgacgcc aaacgcttta acattcatga cgatcagaaa atggtgctgg
    20821 atttcctgtt gcaggaaaaa gttctgttgg tgcaagggac ggcattcaac tggccgtggc
    20881 cggatcactt ccgcattgtc acgctaccgc gtgtcgatga tatcgagctg tctttgagca
    20941 agttcgcgcg tttcctttct ggttatcatc agctgtaatc ttaatttcac tgccggagat
    21001 tgcatccggc agtgttatcc cgccacaatg acctgatgat gtcatcatac gtaaggtcac
    21061 tatgaaacag agccattttt ttgcccatct ctcccgcctg aaactcatta accgctggcc
    21121 gctaatgcgc aacgtgcgga cggaaaatgt gtccgaacac agtttgcagg tagcgatggt
    21181 cgcccatgcg ctggcagcta tcaaaaatcg gaaatttggc ggtaatgtca acgccgaacg
    21241 tatcgcttta ctggcgatgt accacgatgc ctcagaagtg ctcaccggcg atctccctac
    21301 tccggtgaaa tacttcaatg cgcaaatcgc tcaggagtac aaggctattg aaaaaatcgc
    21361 tcagcagaaa ctggtcgata tggttccgga agaactgcgg gatatctttg cgccgttaat
    21421 tgacgagcat gcatatagcg atgaagaaaa atcgctggtg aaacaggcag atgcactgtg
    21481 tgcatatctg aaatgtctgg aagaactcgc ggccggaaat aatgaattct tgctggcaaa
    21541 aacgcgactg gaagcgacgc ttgaagcgcg tcgcagccag gagatggact acttcatgga
    21601 agtatttgtt cccagcttcc atctttcgct cgatgagatt agccaggatt caccgctgta
    21661 agcagccgga gtctgcgtcg catcaggcaa taagcgccgg atgcgacatc aggctcttgt
    21721 caaaacggaa acagcatcgg gatcatcacc acacaaaccg ccatcacgat aatggtgaac
    21781 ggtaccccca acttcacaaa gtcactgaag ctgtaatttc ccggacctaa aaccagggtg
    21841 ttaacaggtg aagacaccgg tgtcataaag gcggcggatg ctgccatcgc cacgaccatc
    21901 gcaaatggat aaggcgacac tcccatcgtt ttggcagcag ccagcgcaat cggagccatc
    21961 aacaccgccg tcgcggtatt agagataaac agcccaataa ccgccgacaa gacaaacaaa
    22021 caccccagca tcatatgtgg cccataaccg ccgccaatat ccatcagccc tttcaccgcc
    22081 agcgcgacac ctcccgtttt ctgtaatgcc acagcaaacg gcatcatccc aacgatcaaa
    22141 ataatgctcg gccagtgaat ggatttatag gcgctttcag catctataca gcggaatttc
    22201 cccatcagca ggcaggcgat gatagcggca acaggattag gaatttcatt tgtcagcatt
    22261 aacgccacca ttagcaccag acagaaaatg gcatggggtg cctggctgtg cgcgggcgat
    22321 gcttcactca cctcttccgg taagttcagc gctacgaagt cgcggccctg tttggccagc
    22381 ataccgatca gtttccagtt acccacaacc aggatgatat cgcccagcag cagaggctca
    22441 tccgccagcg aaccttccag cgccacgcca ttgcgcttga gccccaccac attcagtccg
    22501 tagcgggtac gaaaaccaat ttcgcgcacc gatttaccaa tcagttctga ctcaggaatt
    22561 aatgaaatct ctgccatgcc cacatcaagg gcctggtcag aaaaatactc gccgcgcagt
    22621 accatcggct ccagcaattg ctcactacaa aattgccgga gatcgacatc agccgcagac
    22681 atatcaataa gcaaaacgtc acgcgcgcga aattcagaaa ccccattaac gttcacgata
    22741 acgcgacgaa aacgccgcca gcgttcaaca ccgatgacgt tagcgccata acgctcacgt
    22801 aatttgagat catccagccg ttgacctatc attggcgatc cggggcgaat agccagacgt
    22861 cgcgcacgcc cggtcagtcg atattcacgg ataagatcgc gaaaggttcg acgcgtccag
    22921 ccttcgcgct gcggggtctg ggtatcccct ttcagcatga aacgcatcac taacatatac
    22981 aaaataccca gcaccagcac gaccaggcca ataggtgtta cgctaaagaa actgaagccg
    23041 tgatagcctt cacgcagcaa ttcactgttg actaccaggt tcggcggcgt cgccaccagc
    23101 gtcatcatgc cgctaatcag cccggcaaaa cttaacggca tcatcagacg tgacggcgaa
    23161 gtttgcatac gcatggcaac gcttaacacc acgggaataa agatagcgac aacgctggtt
    23221 gaactcataa acgcgccaag ccccgcgacg gtcagcatca acaaaaccaa cattttgatt
    23281 tcactgttgc ccgccacttt gaccagccat gttcccatta cggtggcaac accggtacgg
    23341 accaaaccat cgccaataat aaacaaggcg gcaatcagga caacgttagg atcagaaaag
    23401 ccggaaaata cttcggggac cgtcagcgtt ccgcttaatg caaacgcgac aataacaaac
    23461 aaagcgaccg catccatacg cactctgccc gtcgcaaaca agacgatggc aacggccagt
    23521 aatgaaagaa cccaaatcaa ttcaccgttc acaacttatc cttgttaatt gagggggatg
    23581 acttgattct gccataaaaa agccccgacg agacggggct aaatcatgat caggtgtttc
    23641 actgaataat aacatcgcca tttggctgtt tggtcacagt aatttgctcc agactgtgga
    23701 ggaccaaatc gacctcattc aggcgcgggg tatctgccgg agcgttaacc gcaatgacat
    23761 ggcaacccgc cgctaggcca gaaagcacgc cagcgggagc atcttccacc accacacact
    23821 cctgcggcgc aagccccagc agctgcgcac ctaacagata cgcatcaggt tctggttttc
    23881 cgcgcttcac tcgctcagcg gttacaaaca cctctggtgc gggaagcccc gctattttat
    23941 ggcgcgctcg cgctaccggc atggagccag aagtcacaat ggcccacgga atacctgctt
    24001 tattcaaatg attgagtaag gcgattgccc caggaagcgc ggtaatacct tcggtttccg
    24061 tggcctcgat ttgctccaaa cgcgtaaact cggcggcaat atcagcctcg gatttgcccg
    24121 ccataaaatg gcgcaaagag gtgatcgcct gtttaccgtg aatgaaagcc agcacctctt
    24181 ccggcgctaa cccatgacgt ctggcccagt tgctccacgc ccgttctacc gcaggcaggg
    24241 aatccaccag cgttccatca agatcaaaca gaaaaccttt gcaccgcacg cgggcctcct
    24301 caggcgttga taatttgatt aatttcgttg gcgctcaaat ggtactgacg cgggcaggca
    24361 tgccacacat taagcatgcg ctgatatttt tcccacattg gcgtctgggc gttaaaacca
    24421 tgagtcccag cgtcaaaatg ggtatagcgc ccttccacat taaccataaa gcggacataa
    24481 ccgaggtaac gtgcttcagt ggcggcgtca aagccgagga aggtgacacg acgttcatcg
    24541 atggattgct ggtcctgcaa attagaccag gaaacatgca acgcatgata catctccata
    24601 atgtcgatga tagtgcggca ggtttcttct ttcagctcgc caaactcgcg atccaattca
    24661 cgcatctgta atccgtaacc acgctcaata attgtttgca ggcgacggta acgttcagca
    24721 tttgccggat cgagcatagt catcatcttg tactgattag acaaaataag acgttgcgcg
    24781 ttggtcattt ccattgttga ctcctgtatc actctactac ggtgaaaaaa aagaaggctg
    24841 agtatgcctt cttttatatg cgtaatcagg ggtcaattac aaatcatcaa ggaaagtttt
    24901 atccagttgt ttgaaggcgc gcttaagcgt gtcagctaat gcctggtaat caggcttgcc
    24961 ttcaacgggt gccaacacct gtccagactc ctgcaattta ccgcgaactt cataaaacca
    25021 gttaaggatt gcagggggta atggcgttac agaacgcttg cccagccacc acaatccctg
    25081 catgggtaaa cttaaggcga acagtgcagt ggcaactgcc ggcccaagca gaccgcccag
    25141 ggcaatctgc cagcagagag taaatacggc gatcggcggc ataaaacgga tcgcataacg
    25201 cgtcatcttg ataacgcgat tttcgacaaa gaccggggca aggcgttttt ccagtggcca
    25261 cgtctttgag taatgctgtc cgcggcgaaa caagctaaaa aaattaacag aacgattatc
    25321 cggcgttgac atgcttcacc tcaacttcac atataaagat tcaaaaattt gtgcaaattc
    25381 acaactcagc gggacaacgt tcaaaacatt ttgtcttcca tacccactat caggtatcct
    25441 ttagcagcct gaaggcctaa atagtacata ttcattgagt cgtcaaattc atatacatta
    25501 tgccattggc tgaaaattac gcaaaatggc atagactcaa gatatttctt ccatcatgca
    25561 aaaaaaattt gcagtgcatg atgttaatca taaatgtcgg tgtcatcatg cgctacgctc
    25621 tatggctccc tgacgttttt ttagccacgt atcaattata ggtacttcca tgtcgagtaa
    25681 gttagtactg gttctgaact gcggtagttc ttcactgaaa tttgccatca tcgatgcagt
    25741 aaatggtgaa gagtaccttt ctggtttagc cgaatgtttc cacctgcccg aagcacgtat
    25801 caaatggaaa atggacggca ataaacagga agcggcttta ggtgcaggcg ccgctcacag
    25861 cgaagcgctc aactttatcg ttaatactat tctggcacaa aaaccagaac tgtctgcgca
    25921 gctgactgct atcggtcacc gtatcgtaca cggcggcgaa aagtatacca gctccgtagt
    25981 gatcgatgag tctgttattc agggtatcaa agatgcagct tcttttgcac cgctgcacaa
    26041 cccggctcac ctgatcggta tcgaagaagc tctgaaatct ttcccacagc tgaaagacaa
    26101 aaacgttgct gtatttgaca ccgcgttcca ccagactatg ccggaagagt cttacctcta
    26161 cgccctgccg tacaacctgt acaaagagca cggcatccgt cgttacggcg cgcacggcac
    26221 cagccacttc tatgtaaccc aggaagcggc aaaaatgctg aacaaaccgg tagaagaact
    26281 gaacatcatc acctgccacc tgggcaacgg tggttccgtt tctgctatcc gcaacggtaa
    26341 atgcgttgac acctctatgg gtctgacccc gctggaaggt ctggtcatgg gtacccgttc
    26401 tggtgatatc gatccggcga tcatcttcca cctgcacgac accctgggca tgagcgttga
    26461 cgcaatcaac aaactgctga ccaaagagtc tggcctgctg ggtctgaccg aagtgaccag
    26521 cgactgccgc tatgttgaag acaactacgc gacgaaagaa gacgcgaagc gcgcaatgga
    26581 cgtttactgc caccgcctgg cgaaatacat cggtgcctac actgcgctga tggatggtcg
    26641 tctggacgct gttgtattca ctggtggtat cggtgaaaat gccgcgatgg ttcgtgaact
    26701 gtctctgggc aaactgggcg tgctgggctt tgaagttgat catgaacgca acctggctgc
    26761 acgtttcggc aaatctggtt tcatcaacaa agaaggtacc cgtcctgcgg tggttatccc
    26821 aaccaacgaa gaactggtta tcgcgcaaga cgcgagccgc ctgactgcct gatttcacac
    26881 cgccagctca gctggcggtg ctgttttgta acccgccaaa tcggcggtaa cgaaagagga
    26941 taaaccgtgt cccgtattat tatgctgatc cctaccggaa ccagcgtcgg tctgaccagc
    27001 gtcagccttg gcgtgatccg tgcaatggaa cgcaaaggcg ttcgtctgag cgttttcaaa
    27061 cctatcgctc agccgcgtac cggtggcgat gcgcccgatc agactacgac tatcgtgcgt
    27121 gcgaactctt ccaccacgac ggccgctgaa ccgctgaaaa tgagctacgt tgaaggtctg
    27181 ctttccagca atcagaaaga tgtgctgatg gaagagatcg tcgcaaacta ccacgctaac
    27241 accaaagacg ctgaagtcgt tctggttgaa ggtctggtcc cgacacgtaa gcaccagttt
    27301 gcccagtctc tgaactacga aatcgctaaa acgctgaatg cggaaatcgt cttcgttatg
    27361 tctcagggca ctgacacccc ggaacagctg aaagagcgta tcgaactgac ccgcaacagc
    27421 ttcggtggtg ccaaaaacac caacatcacc ggcgttatcg ttaacaaact gaacgcaccg
    27481 gttgatgaac agggtcgtac tcgcccggat ctgtccgaga ttttcgacga ctcttccaaa
    27541 gctaaagtaa acaatgttga tccggcgaag ctgcaagaat ccagcccgct gccggttctc
    27601 ggcgctgtgc cgtggagctt tgacctgatc gcgactcgtg cgatcgatat ggctcgccac
    27661 ctgaatgcga ccatcatcaa cgaaggcgac atcaatactc gccgcgttaa atccgtcact
    27721 ttctgcgcac gcagcattcc gcacatgctg gagcacttcc gtgccggttc tctgctggtg
    27781 acttccgcag accgtcctga cgtgctggtg gccgcttgcc tggcagccat gaacggcgta
    27841 gaaatcggtg ccctgctgct gactggcggc tacgaaatgg acgcgcgcat ttctaaactg
    27901 tgcgaacgtg ctttcgctac cggcctgccg gtatttatgg tgaacaccaa cacctggcag
    27961 acctctctga gcctgcagag cttcaacctg gaagttccgg ttgacgatca cgaacgtatc
    28021 gagaaagttc aggaatacgt tgctaactac atcaacgctg actggatcga atctctgact
    28081 gccacttctg agcgcagccg tcgtctgtct ccgccagcgt tccgttatca gctgactgaa
    28141 ctggcgcgca aagcgggcaa acgtatcgtt ctgccggaag gtgacgaacc gcgtaccgtt
    28201 aaagcagccg ctatctgtgc tgaacgtggt atcgcaactt gcgtactgct gggtaatccg
    28261 gcagagatca accgtgttgc agcgtctcag ggtgtagaac taggtgcagg cattgaaatc
    28321 gttgatccag aagtggttcg cgaaagctat gttggtcgtc tggtcgagct gcgtaagaac
    28381 aaaggcatga ccgaaaccgt tgcccgcgaa cagctggaag acaacgtggt gctcggtacg
    28441 ctgatgctgg aacaggatga agttgatggt ctggtttccg gtgctgttca cactaccgca
    28501 aacaccatcc gtccgccgct gcagctgatc aaaactgcac cgggcagctc cctggtatct
    28561 tccgtgttct tcatgctgct gccggaacag gtttacgttt acggtgactg tgcgatcaac
    28621 ccggatccga ccgctgaaca gctggcagaa atcgcgattc agtccgctga ttccgctgcg
    28681 gccttcggta tcgaaccgcg cgttgctatg ctctcctact ccaccggtac ttctggtgca
    28741 ggtagcgacg tagaaaaagt tcgcgaagca actcgtctgg cgcaggaaaa acgtcctgac
    28801 ctgatgatcg acggtccgct gcagtacgac gctgcggtaa tggctgacgt tgcgaaatcc
    28861 aaagcgccga actctccggt tgcaggtcgc gctaccgtgt tcatcttccc ggatctgaac
    28921 accggtaaca ccacctacaa agcggtacag cgttctgccg acctgatctc catcgggccg
    28981 atgctgcagg gtatgcgcaa accggttaac gacctgtccc gtggcgcact ggttgacgat
    29041 atcgtctaca ccatcgcgct gactgcgatt cagtctgcac agcagcagta atctcgtcat
    29101 catccacagc tttgcgctgc ggatatctga accggaaata atcactattt ccggtttttt
    29161 attctcttaa tttgcattaa tcctttctga ttatcttgct taactgcgct gcatcaatga
    29221 attgcgccat ctcactttgc atacttacca ctttgttttg tgcaagggaa tatttgcgct
    29281 atgtccgcaa tcactgaatc caaaccaaca agaagatggg caatgcccga tacgttggtg
    29341 attatctttt ttgttgctat tttaaccagt cttgccacct gggtagttcc ggtggggatg
    29401 tttgacagtc aggaagtgca gtatcaggtt gatggtcaaa caaaaacacg caaagtcgta
    29461 gatccacact catttcgcat tctgactaac gaagcaggcg aacctgagta tcaccgcgta
    29521 cagctgttca cgacgggcga tgaacgcccg ggcctgatga acttcccgtt tgaagggtta
    29581 acctcaggat cgaaatacgg gacagccgtt ggcatcatca tgtttatgct ggtgattggc
    29641 ggcgcgtttg gcattgtgat gcgtacagga accattgata acggtatcct ggcgcttatt
    29701 cgccataccc gcgggaatga aattctcttt attcctgcgc tgtttattct gttttcactt
    29761 ggcggcgcgg tatttggtat gggagaagag gccgtcgcct ttgccattat catcgcaccg
    29821 ctaatggtcc ggctgggcta tgacagtatt accaccgtcc tggtgactta tattgccacg
    29881 caaatcggtt ttgccagttc gtggatgaac ccgttttgtg tggttgttgc tcaggggatt
    29941 gccggcgttc cggtgctttc tggctccggg ttgcgcatcg tggtgtgggt tatcgccact
    30001 ctgattggcc tgatctttac catggtgtac gcctcacgag tgaaaaagaa tcctcttctg
    30061 tcacgcgtgc atgagtccga ccgcttcttt cgtgaaaagc aggcggatgt tgaacaacgt
    30121 ccgtttacct ttggtgactg gctggtattg attgtcctga cagccgtaat ggtctgggtg
    30181 atttggggcg tgatcgttaa tgcctggttt attccagaaa ttgccagcca gttcttcacc
    30241 atgggtctgg tgattggcat catcggcgtt gttttccgcc ttaacggcat gacggttaat
    30301 accatggctt catcctttac cgaaggggcg cgaatgatga tcgcccctgc cctgctggtg
    30361 ggtttcgcca aagggatttt gctgctggtt ggtaatggtg aagcaggtaa tgccagcgtg
    30421 ttaaatacta tcctcaacag cattgccaat gccattagcg gtctggacaa cgcggtcgcg
    30481 gcctggttta tgttgctctt ccaggctgta tttaatttct tcgtgacgtc cggttctggt
    30541 caggcggcat taactatgcc gttactggca ccgcttggcg atctggtcgg tgttaaccgt
    30601 caggttaccg tgctggcgtt ccagtttggt gatggcttca gccacatcat ttacccaacc
    30661 tcagcttcgt taatggcaac gctcggtgtg tgccgggtgg acttccgtaa ctggctgaag
    30721 gtgggcgcga ccctgcttgg actgctgttt attatgtcca gcgtcgtagt gatcggcgct
    30781 cagttgatgg gctaccacta aaaatgttaa gagccgcaat tgcggctctt ttttcattct
    30841 gccgtttcag tctctacggc ttcatttttg gcattgcgct tcatccacaa cgccagcgct
    30901 ttcagcgagt ctggagtgaa ctcatcgcag cgtgcggtga tttcttccgg cgtcagccag
    30961 caaacttcac tgacttcatc ttcctgtagg gcgaagggac cgtgagagac gcaactgaac
    31021 aacgcgcccc agacacggca atttttatct tcgaaataga actgcccgtg ctcggcaaag
    31081 gggacaccgg caatgcccaa ctcttcttcc gcttcgcgac gcgcggattc cagcagttgc
    31141 tcatcggcct gaactacacc gcctgcggtc gcatctaaca tgccgggtaa aaagtctttt
    31201 gtctcggtac gacgctggac cagaattttg cccatgccat catgcacgac gatgtaagtt
    31261 gcacgatgac gcagacactg tgcccgcatt tgttcccggc tggcttgtgc aatgacttcg
    31321 ttctcttcat tgacaatatc cacccattca gtacttgcca aacgacgctg ttccattatc
    31381 aggaaacctt ctttttctgg cgctcttacg gcgcattttg agttgtgggt aaattacgga
    31441 ttaatcgcga cctgcgcaat gatactttga tcattgagtg cgattacact aagtacgtca
    31501 ttatccagca tgccataact cgccggatta ccgcctttcg gaatactcac cgagccgggg
    31561 ttgaagtgga aaatctcccc ccgctgttct gccactggca gatgggtatg accgtacacc
    31621 agcacatcgt tctggtttaa agcaggtaga ttttccgggc caaaaagatg accatgcgtc
    31681 aaaaacagac gttgtttttc cagtaatacc tgttgccacg gcgcggttat cgggaaatgc
    31741 agcatcattt gatccacttc gctgtcgcag ttgccacgca cagcgataac cttatgtgcc
    31801 acttcattaa gccgttcagc aactttggct ggcgcgtaac cctccggtaa agcattacgc
    31861 gggccgtgat tcaacacgtc gccaaggatc accagccact gggcaccgct ttggacaaac
    31921 aactccacaa cacgttccgt cgccggtaac gacccatgaa tgtccgatgc aaacatcagt
    31981 ttcatcactc actcctcgtc gaaaaaacgt aagctatgat actggattct gtcgttgcta
    32041 tcagccagat tgcttcgccg aaagtgcaat aaaacgctgg acagacgctc gctgccactg
    32101 gaatgtcgca taatccacca ggcgttctgg cacctcatcg ccatgtagta ccaggcggtt
    32161 aatcattagc gccagatcag tatcagcaat gcaccattca ccaaataaat tcggctgacc
    32221 cagtgctaac aaatgttctg ccatcgcgaa cagtttctct gcactggctt ttccctcggc
    32281 cgttagtggc gcttttttcg cccccgcaaa gacaacatcc gtcggacgct cttcgcggat
    32341 gggcatcaga tcgctgcgca gccaggcctg aatctgtcgt gcacgcgcac gattttctaa
    32401 atcaagcgga taaatacgtt cccaggtcgg tggcgcaaat cgatcttcca gatactccgc
    32461 aatggcagaa gattcactca actcaaaatc atcgatttgt aataacggca cacggcgtgt
    32521 ctgaccgtaa ccttgccacg tcggctgcaa atgttcaccg ctgtcgaggt cgatggtctt
    32581 gatatgaaac gacaggcctt tttcctgcaa cgccacccag gcggataaca cataagggga
    32641 gaaaaagtgg gcatctgacc aaagcgtgat agcgggttta ctcattatgt cctcggcaat
    32701 aaagggggct ggctatcaaa ctatagagcc tttttgtcac tgtcacgtga cgaatactca
    32761 cgtaagtcgc cgcgctttct atacttgtgg tgttcagatg attaaacaac cggagctgca
    32821 atgatcgatc tctatttcgc cccgacaccc aatggtcaca aaattacgct gtttctcgaa
    32881 gaagcagagc ttgattatcg cttgattaag gtagacctgg ggaaaggcgg tcagtttcgt
    32941 ccggaatttt tgcgcatttc gcccaacaac aaaattccgg caattgttga tcattctcct
    33001 gccgatggcg gcgaaccgtt aagcctcttt gagtctggtg ccattttgtt gtatctggct
    33061 gagaaaacag gactcttttt gagtcatgaa acgcgtgagc gcgccgccac attacagtgg
    33121 ttattctggc aggtaggcgg actggggccg atgcttgggc aaaatcatca ttttaatcac
    33181 gcagcccccc aaaccattcc ttacgctatt gaacgttatc aggttgaaac tcagcgtctt
    33241 taccatgtac taaacaagcg gctggaaaac tcgccctggc tgggaggcga gaactacagc
    33301 attgcggata ttgcctgctg gccgtgggtt aatgcctgga ctcgcctgcg aattgacctc
    33361 gcaatgtatc cggcagtcaa gaactggcat gagcggatcc gttcgcgccc tgccaccggg
    33421 caggcactgc taaaagcaca actcggtgat gagcgttcgg atagttaaca gaaacaggtt
    33481 ctcgtgtatt atttcatcct aagtaaaaca acggagaacc tgcaatggca caacctgccg
    33541 ctattattcg tataaagaac cttcgtttgc gtacgtttat cggaattaag gaagaagaaa
    33601 ttaacaaccg tcaggatatt gttatcaatg tgacgatcca ctaccccgcc gataaagcgc
    33661 gcactagcga agatatcaac gatgcgctga attatcgcac cgtaacgaaa aacattattc
    33721 agcatgtaga gaataaccgt ttctctttgc tggaaaaatt aactcaggat gtgctcgata
    33781 tcgtacgtga acatcactgg gtgacgtatg ctgaagtgga gatcgataaa ctgcacgcgc
    33841 tgcgctacgc cgattcggta tccatgacct taagctggca gcgttaatcg ccatatcggg
    33901 aggctgcatg aacatagtga tcaccggagg gacgggatta attggtcgcc atttgattcc
    33961 gcgtttgctg gagctgggcc atcaaattac ggtagtgacg cgtaacccgc agaaagccag
    34021 ttccgttctc ggccctcggg tgacactatg gcaagggctt gccgatcaaa gcaacctcaa
    34081 cggcgttgat gcggtaatca acctggccgg agaaccgatt gctgataaac gctggactca
    34141 caagcaaaaa gagcgtctct gccaaagcca ctggaatatc acgcaaaaac tggtcgattt
    34201 gattaatgcc agcgacacgc cgccgtcggt actcatttcc ggctcggcaa cgggctatta
    34261 tggcgactta ggtgaagtgg tggttaccga agaggaaccg ccgcataacg aatttactca
    34321 taaactctgc gcccgctggg aagagattgc ctgccgggcg caaagtgaca aaacgcgagt
    34381 gtgcctgctg cgaaccggcg tggtactggc accggacggc ggtattctcg gtaaaatgct
    34441 gccaccgttt cgtcttggcc tgggcgggcc gattggttcc ggtcggcagt atctggcctg
    34501 gattcatatt gatgatatgg tcaacggcat tctctggctg ctggataacg agctgcgcgg
    34561 gccatttaat atggtttcgc cctacccggt acgcaatgaa caatttgccc atgcgctcgg
    34621 tcatgcgctg catcgcccgg ctattttgcg cgtccccaca accgccattc ggctgttaat
    34681 gggcgaatct tcagtactgg tattaggtgg acaacgcgcg ctgcctaaac ggctggaaga
    34741 agcgggtttt gcgtttcgct ggtacgattt agaagaggcg ctggcggatg tcgttcgctg
    34801 atgtggttta cagcaaacat ccgccagtta actccctgtg ttacaggatt agtggctttg
    34861 cgcgataaga tcgtctggtg aaagtcgggt caccatcata actaactctc tgtctaaacc
    34921 tctatccagc atctcctgag caatacgcag ggcttcttcg tgtttgccct gcattgcgcc
    34981 ttcttcacgt aatctgtcag caatggtcat cagtttctcc ttttcttgtg gtgcgcgttc
    35041 tgttatctca ccaataaatg cacggaaacg ctgggcatcc cctgtttgta atacgtaatt
    35101 aaacagggct tttagctgtc tgtcattagt gttccctgta actagcagcg aaacaatttg
    35161 gtcgactaat cccaacagat cgcgctgacg aatatgtttc tgaattaact ccaacagcgc
    35221 cattttgcgg tgttgcataa tctcgtcatc cggcaccacg gtaatatcca ccaacggaaa
    35281 cgctgatgaa tatattttgc gggctatagc aggctcggca aattcatcaa gccagcagag
    35341 tgaataagga taaggacttc tgcaaccatg ataaaacagc atcgggatca ccaatggaag
    35401 ctctttatag cccgcatcaa gatggttttg cattgccgca atggaataac gcatcatgcg
    35461 aaaagccatt aattcttccg gcttactttg gtgctctatc actacataaa tataacccac
    35521 tccctcctgc gttttcacag accacaagag gtcggaataa tattgccgca ggtcttcatc
    35581 aataaaactg tttggttcta gtttaagcgt cgttaaatca cacagtttgc gcagcggcgc
    35641 gggaagatga atattaataa aattccgcgc ggtgtctgga tggcgtaaaa aagatttaaa
    35701 taccgcatca tgcggcgtgg aagttgtcga tattgtcatg gcgttccgtc acccctcaaa
    35761 tttaagatga cgcgacaata accacgatta acacccaatg cagcaggcag tttttatctt
    35821 tggacagcga attcagaagt atttttagcg gactgcaact tttacatttc agcggaagct
    35881 gactcgcaaa ttccccttga aaatactccg gcagcgttag ccaccggaga ggaaattatt
    35941 tcagcgatcc cttaaggaac tgttgcagac gagggctttg cgggttgcca aataactgct
    36001 ccggcgcgcc ctcttcttct attttcccct gatggaggaa aatgacgtga gtagaaacat
    36061 ggcgagcaaa gcccatttcg tgagtcacta ccaccatggt tttcccctct tctgccagtt
    36121 gctgcataat acgtaacact tcgcctacca gttcaggatc gagcgccgag gtaggttcat
    36181 caaacagtaa aacttctggt tccatcgcca gcgcccgcgc gatagaaaca cgctgttgct
    36241 gaccgccgga aagatgcacc ggatatttcc cctgcgcacg ttcgtctatc ccgactttcg
    36301 ccagatactt caccgcccgc tcgcgcgctt cctgcttgct caggcccaac acctgaatcg
    36361 gcgcttccat gacgttttcc aacaccgtca tatggctcca gagattgaag tgctggaata
    36421 ccatcgtcag gcgtgtgcgc agtaagcgca gttgattttt atcggcgact ttgagttgac
    36481 cgtctttgtc gcgcaccaga ttgatcgtct ggccattgac cacgatcgat ccttcactcg
    36541 gtttttcgag gaagttaatg cagcgcagaa aggtactttt ccccgatccc gacgatccga
    36601 tgatgcttat tacatctccg gcattcgctt gcagtgagac ccctttcagc acttcgtgtt
    36661 cgccatagcg tttgtgcaaa tcgataacgt ttaatttatt ctcggacatc gtattctcag
    36721 tgcgttgaag aaggtttcac atgctgcaac cagcgttttt ccgctctgcg aaagaggctg
    36781 atcaggacat aagagatgat taaatagagc accgcggcaa tgccgaaggc ggtaaaaggt
    36841 tgatacgtgg cggcgttaat atcgcgggct attttcagca aatccggcac agtggccgta
    36901 aacgccagcg cagtagagtg cagcatcagg atcacttcgt tgctgtatgc cggtaacgca
    36961 atacgcagcg cagaaggcaa aataatgcag cgatacattt taaaggtcga gaagccatag
    37021 gctctggcgg cttcaatttc cccatgcgga accgaacgga ttgccccagc aaaaatctcg
    37081 gtagtgtaag cgcaggtgtt aagcgtcagc gccagcacgg tacagttcag gccactgcgg
    37141 aagaaagcgt taaggaattc ggttccctta acaatctcaa gcgtgtacat gccggaatag
    37201 aacaccagca actgaacata cagcggcgta ccgcgaaaaa tataggtaaa taaccagatt
    37261 ggaaactgga tgtatttatt actggagaca cgaccaatcg ccagaaacag cgccaggact
    37321 ccgcctatca ctaccgacaa aataagcagc cacagagtga tcgccacacc agtaaagcga
    37381 taaccgtcgg tccacagcag cggtttccag tattcatgta agatttcgat cacaggtcag
    37441 ccctcttcac acccacggag tagcggcgct caaggaacag cagcacacca ttggaaacgg
    37501 tggtgaaaac caggtaaatc acgccacaga cgatggcgaa atagaacggt tcccaggtac
    37561 ttttgcctgc cagctgcgtg gctttgacca catcttccag gccgagtaac gaaaccagtg
    37621 cggtagattt gaggatcacc tgccagttgt tgccaatgcc tggcagggcg taacgcatca
    37681 tcgccggaaa catgatcctc cgaaacactt gcccgcgagt aaaaccgaac gccgtcgccg
    37741 cctctatatg ccctttcggc actgccataa acgcgccacg gaacgtttcg gtaaaataag
    37801 caccgtagat aaaaccgaga gtgataatac cagcgaccat cggatcgata tcaatctgcc
    37861 cgacgcccat cgcctccgtc accatgttta gcgcaatctg caaaccatag aaaatcagca
    37921 gcatcaacac caagtccggc acgcctcgaa tcagcgtggt gtacccttcg aaaatcagcc
    37981 ccaaaagccg attttgcgag agcttaccgc cagcgccaat taaaccgatg attacagcga
    38041 gcactacaga gctgatagcc agctccagcg tgacgagcgc accctgtaaa ataacacctg
    38101 aaaacccata caacatgctg cctgtcctgt cgtgtgtggt gaattaccgc ctcttgtctc
    38161 cctccggtta cccgaaggga gcgggctttc tttcaccgat ggcgattaac caccataaac
    38221 atcaaaatcg aagtactttt tcgctaattt ctcgtaagta ccgtcagcgc gcatttcggc
    38281 aaaggctttg ttcagtgcct cgcgcagttc gttatcttct ttacgcaggc ccatgccggt
    38341 ccctacgcca aatagttttt catctttaac agacggacca ccgaatttgt aatctttacc
    38401 gacgggttgt ttgaggaaac cttcgctggc agcgacctca tcctggaacg cggcatcaat
    38461 acgtccggca gtcaggtcag aataaatgtt gtcctgcccc tgatacgaga cgatttcaat
    38521 gccttttggt gcccagtgtt cattaccgaa cgtctcctgg gtggtgccct gcaatacgcc
    38581 tacccgtttg cctttcagcg actcgactgt cggctgaatg tcagaatttt tcgccaccac
    38641 cagacgagaa tcggcagcgt acagtttgtc ggtgaaggca atttcttgct gacgtttttc
    38701 cgtaatggaa agcgatgaca tgatggcgtc aatcttcttc gcttttaagg acgggattaa
    38761 cgcatccagc gggttttcga caaacgtaca ttgcgtattg atgcgcttgc acaactcctt
    38821 cgccagctcg atatcgaaac caaccagttc gccttgtgaa ttttttgatt caaatggcgc
    38881 ataggtcggg tcggtaccga tgcggatgtt ttgcggaatt gcagcaagtg ccgcagttgc
    38941 gctggagaag gccagaacca gagagagcga tagcaccagt tttttcataa tgtcctcaac
    39001 tgacagtctt tttataggga atatttacag gtttgttaca cttatcgtgc cataaaattg
    39061 accatcaagg caaaatatcc cgccccgtta gacgtattat ttttcattaa aaatgcttaa
    39121 gtatgcattt caatgcacca cgatagtgca acaaacctgg cgtgcaccat gatgatcatt
    39181 ccatcaggta cagcttccca gcgacgtatc agtcaccgta gacattaaag tcgaaatact
    39241 ttttcgccat cttgtcgtag gtgccgtcct gacgcagctc gccaagcgcc ttattgaagg
    39301 cagccgtcag ttcagcatca tctttacgta gccctacacc ggtgccatca ccgaagtatt
    39361 ttttgtcttt tactgatgag ccagcaaagg cgaaatcttt accagcaggt tgcttgagga
    39421 atccttcgct ggcagcaact tcatcttgta acgcagcatc cagacgtcct gcagccagat
    39481 cggaatagac caaatcctgg ttggcatagg ccaccacatc cacgccttta ctacgccagg
    39541 tctcgttagc gtaagcttcc tgggttgatc cctgcagcac accaacatgt ttacctttca
    39601 gtgaatccag cgttggctga atcggtgaac ctttggcagc aatcaaacga gaatctgcgg
    39661 cgtacagctt gtcggagaag gcaatctcct gctgacgttt atcggtaatg gaaagcgacg
    39721 aaataatagc gtcgattttt ttcgctttca gtgaggggag cagcgcgtca aagtcactgg
    39781 caacccaggt acatttcacc tgcatccgtt tgcacatctc gttaccgaga tcgatatcaa
    39841 agccaacaaa atcaccttta gcatctttcg acgagaacgg cgcataggtg gtatcggttc
    39901 cgatacgtac cgtctccggt agcgccgcat agctggaagc cgctgcggag agaccgacta
    39961 ataaagacag agcgagaatc gacttcttca tacataaccc tcaagtgaaa atggcttttt
    40021 tatgttgtgt attgtgttgt gtgtttgcag gctctttcat gcaggtctta tgccatcttg
    40081 acgacaacag taacattcaa cgttaaatat gttaataaga cgttgcatta ttgtcctgaa
    40141 gttgaagata gcaggtatag cggttgaatc gcagcgtttc gattgaatgg cagaaacaaa
    40201 atgtcgagga tttgatcgcg gttgcaaaat tgccctgaaa cagggcaaca gcggagttat
    40261 gcgccctgcc agcgggcaaa gagatcttca ggaagggtta ttgcaaactg gtcaagaaca
    40321 cgattaaccg tctgatttat cacatcatca agggattgcg ggcgatgata aaacgccgga
    40381 acgggaggca taatcaccgc accgatttct gccgcctgag tcattaaacg cagatggcct
    40441 aagtgcaatg gtgtttcacg cacgcagagc accaacgggc gacgctcttt cagcaccaca
    40501 tctgccgcac gggtcagtaa gccatcagta tagctatgga caatgccgga cagggttttg
    40561 attgaacagg gtaaaatcac catccccagc gtctggaaag aaccggaaga gatgctggcg
    40621 gaaatatcgc gcgcatcgag cgtgacatca gctaatgcct gcacttcgcg cagggaaaaa
    40681 tccgtttcga gggataaggt ctggcgcgcc gcctggctca tcaccagatg cgtttcgata
    40741 tctgtgacat cgcgcagaac ctgtaataag cgcacgccat aaatcgcgcc gctggcaccg
    40801 ctgatgccta caatgagtcg tttcataaat agttgccctg tcagacttgc aggcagactt
    40861 tgcagtattt cggcgggagt tgcaagtcag ggtgccagac cggcaccctc agcgaaggca
    40921 tcatccttcg ttatgcattt cgagattttc cacttcgttc tgacgttgca ctgctttggc
    40981 gtcatcatta cgtaacgtat cgaggaaatc gaggtagccc tgatcaacat ctttggtgac
    41041 gtagacgccg ttgaacaccg agcattcaaa ctgctggata tccggatttt cagcgcgaac
    41101 ggcgtcgatc agatcgttca gatcctggaa aatcaacccg tcagcaccga tgatctggcg
    41161 aatttcatca acttcgcgac cgtgagcgat cagttccgtg gcgctcggca tatcaatacc
    41221 ataaacgttc gggaagcgaa tttccggtgc cgcagaagcg aggtacactt tcttcgctcc
    41281 ggcttcgcgt gccatctcga taatctgctc agaagtggtg ccacggacga tggagtcgtc
    41341 gaccagcagg acgtttttat cgcggaactc ggcgcggttg gcattcagtt tacggcgcac
    41401 ggacttacga cgcagctgct ggcccggcat gataaaggtg cggccaacat agcggttttt
    41461 aacgaagccc tggcggtacg gtttgcccag aatacgagca atttccagcg cgatatcaca
    41521 cgaggtttct gggatcggga tcaccacgtc gatatccaga tcttcccatt cgcgggcaat
    41581 tttctcgccc agtttcgtgc ccatattcac acgcgcgctg taaacggaaa ttttgtcgat
    41641 aaacgagtcc gggcgggcaa agtatacata ctcaaacagg cacggattgc tgaccggatt
    41701 gtcagcacat tgacgggtaa acaactgccc ttcttcagtg atgtaaatcg cttcgcccgg
    41761 cgcgacgtca cgcaggaaat caaagcccag cgtatcgagc gctacgcttt cggaagcgac
    41821 catatattct gtacggttct cgtcaatatc acgttttccc agtaccagcg gacgaatccc
    41881 gtttggatcg cggaaagcaa ccataccgtg gccgataatc atcgccacac aggcatacgc
    41941 gccgcggatt aagcggtttg tggcagcaat ggcagcgaaa atattgtcgg cttccagcgg
    42001 gtagtggcgg aagttgtcca gctcgctggc gaagatatta agcagaattt ccgagtcgga
    42061 agtggtgttg atgtggcggc gtttttcttc aaacagtttt ttacgcaact cgtgagcgtt
    42121 ggtcagattg ccgttgtggg caagcgtaat gccatacggg gagttaacgt aaaacggctg
    42181 cgcttcagag gcgctggagc tgccagccgt agggtaacgc acatgaccaa tgcccatatt
    42241 gccctgcaaa cgctgcatat ggcgagcttc aaatacatcg ctcaccagcc cgttcgcttt
    42301 acgcaaacgg aagcagttat tggcatctat ggtgatgatg ccggcggcat cctgaccgcg
    42361 atgctgaagc accgttaagg catcataaat cgactggtta accggcataa caccggcgat
    42421 accgacaata ccgcacatac gtctttttcc tcgttaagcc acatctcaga gcacttacgc
    42481 tctgggcaag aaacttgacg agctttgcag ataatcaaaa aaccatctga tgataaaact
    42541 gaattgcggg atcagctgtg atttgctcca gtcttcactt ttcgacaccc cggtaaagga
    42601 gtcgagaaag aagagaatgg cggcaacaat caacacaccg cgcaacgcac cgaaacagac
    42661 gcccagcacc cgatcggtgc ctgacaaccc cgttttctcc accaactggc ctatcacgaa
    42721 gttcacgata gcaccaacga tcagggtagc gataaacagt accgcgatgg caatcccatt
    42781 tcgaaccagt tcgtcttcaa agcccgtaaa ccagactgac aggtaagtgt agtaatgact
    42841 ggcaacaaag aaagcacaac cccatgtcac cagcgataac gcttcacgaa caaagccgcg
    42901 gatcaggcta accagagagg aaaaagcaat caccgcgatt atggcgtaat caatccagac
    42961 catatgtgtc ccacgatttt acgccctgtc atcctgttcg gggcgcattc taacagaaaa
    43021 agaaaacgtt tgcgtaggga tttccttccc gcgcatcaat aaaaatggcg ctgaaaaaat
    43081 attcaacgcc atcgactttt tatgcctttg cggcatcgtg caatgcgtgt cggatgcggc
    43141 gtaaacgcct tatccgacct acggttctaa cactgcccaa gcctgataag acgcgccagc
    43201 gtcgcatcag gcaagactgt gttaattcgg cgtatagccc attaccacgc cacttaagcc
    43261 agaaagttgc ttcaactcac ccagcgaacc tttcagctta tctttcgagg catccggccc
    43321 aaccagaata cgggtaattt taccctgcac tggcgtggat ggcgacgtat aaacccgata
    43381 accggcaccg cgcagcttac cgacaatctc attcacttta tcggcatttt tcagcgcacc
    43441 cagttgcaca acataggctt tacccgttgg cgctgctttt tcttccacga ccggcttcgg
    43501 ttctggcttc ggcgcaggtg gtgcttcaac ctttggttta ggcggctcca ccggtttcgg
    43561 cttcggtggg gcgaccggtg caggttccgg ttcaaactcg gtgttattgg ctgcaatagt
    43621 ggccggatcg agcgacggtg ctgcggcatc acctgcccgc acctcttccg ctgcgccttc
    43681 cggcggctgc gtcggcaacg cctgggtggc ggctggcatc atatcaggct catcacgatc
    43741 gcccgctttc ggcaccagcg ggatagccgc gaactcatcc tgataatgtt ttttctgccc
    43801 gtccagcagc cctggaagta caatcacccc cagcgccacc agcacgatcg tgcccactaa
    43861 ccgattctga aacttacttg ccaccgcttc tcctcgcgtc aatcacttcc atgacatgtg
    43921 cgaccgtgtg gaaagaacca cacaccagca cggtgtcttc cgctttagcg tccgccattg
    43981 cggcatccca tgcctgcgca acgctatcaa atgatttgcc gttacccaaa tgctcaagca
    44041 gttgttctgc cgtggcaccg cgcggccctt ccagtggcgc acaataccag tcatcaacca
    44101 cgcttttcaa ccaggccaga gttccggcaa tatctttatc atgtagcata ccgataaccg
    44161 ccagcacgcg cccgtttttc ggtagcgctt tcatacgccc ggtgagatat tccgccgcat
    44221 gtggattatg cgcgacatca aaaataacgc gtggcgactc gctcacaatc tggaaacgtc
    44281 ccggcaaaat tgcgctggca atcccgtcgc gaatggcatt ttcactgact tccagcccgc
    44341 tggcacgcag tgccgccagc gccgttgcgg cattcggttg cgggacaagc ggcaacggca
    44401 gattttccag cgtgccgtga gcatcgctaa acgcccagtc atgatcggtg acggaatagt
    44461 tccactcaac gccccgacgt tgtaacagtg cacctttttc ctgcgccaca tcagcaatgg
    44521 tagaaggcat ttccggctca ccgacaattg ccggtttttc gctgcggaag atgcctgctt
    44581 tctcgcgacc aatactttcg cgatctggac ccagccagtc ggtatgatcc agcgcaatac
    44641 tggttactac cgcggcatcg gcatcgacaa tattggttgc gtccagacga ccgcccagcc
    44701 ctacttccag aatcaccacg tcaagttgtg cctgcttgaa cagccacaac gccgacagcg
    44761 taccgtactc gaaataggtc agggaaatat caccgcgtgc cgattcaatc tccgcaaaag
    44821 aggcggtgtg ggccgattcc ggcaattccc ggccctgcac acgtacgcgc tcggtataac
    44881 gcaccagatg aggcgaactg tagacgccca ctttgtaccc tgccgccatc agaatcgact
    44941 ccagcgtacg gcaggtggtg cctttgccat tcgtacccgc aacggtaaac acaaatggcg
    45001 ctggtttcag gacgccaaga cgcgccgcga cctggctcac gcgctcaagg ccgagctcga
    45061 tagttttact gtgcaggttt tccagataag aaagccacga agccagaggc gacgcggctt
    45121 gaggagtgcg tttgataatc atggtatccg ctgattcgtt acggtgagaa tagcaaaagg
    45181 gcagagccag tggccctgcc cttatcagtt atcaggcctc aggttcctga tccggtaccg
    45241 ggggtaccac tacgccttca cgcggcgctt caggattcgg cgctggcaga ttcatcaact
    45301 tcgccagaat gctcgccagt ttcaggcgca tttccggacg acggacgatc atgtcgatcg
    45361 cgcctttctc gatcaggaat tcactgcgct ggaatctagg cggcagtttt tcgcgaacgg
    45421 tctgttcgat aacacgcgga ccggcaaagc cgattaacgc tttaggttca gcgatgttga
    45481 gatcgcccag catggcgaaa cttgcagaaa caccgcccat cgtcgggtcg gtcagcacgg
    45541 agatgtacgg caagccgcgc tcctgcattt ttgccagtgc cgcagaggtt ttcgccatct
    45601 gcatcagcga catcagtgct tcctgcatac gtgcgccacc agaggcggag aagcagatca
    45661 gcgggcagtt atcttcctgc gcctgctcaa cggcacgcac gaaacgtgca cccacaacag
    45721 accccattga accgcccata aaggcgaact cgaatgccgc agcgacaacc ggcattccat
    45781 acagagtgcc tttcatcacc accagcgcat ctttttcgcc ggtttctttc tgcgcagatg
    45841 ccagacggtc tttatacttc ttggagtcac gaaacttcag ctcatctttc ggctcaagct
    45901 cgctacccag ctccacaagg cttccttcat ctaacaggct atgcaggcga ttacgcgctg
    45961 tcatacgcat gtgatggtca cacttcggac agacctcaag attacgttcc agctcagcgc
    46021 ggtataaaac ctgaccgcag ctatcacact tagtccacac cccttcagga atgctcgcct
    46081 tgcgggtggg agtaatgttg cttttaattc gttcaatcca gctcattagg gacctttctg
    46141 tctgaacctg gttcgatgcc agttttatct ttggggacgc ataatgccat ttttgccccc
    46201 aacagaccat gaatgttgca cattaaaaca taacagcccg aaactttgga taaaaaagtg
    46261 gtcgaaccgc ggagttactt tttattttgc ggcgcgtgcc gcagcgcgtt tgtgacggat
    46321 tatttcgatg acgcccggca aaatagaaac cacaataatc ccgacgatca gcagcttaag
    46381 gttatcctga accatcggga ttgtaccgaa gaaatagcct gcgtaggtaa aaagcagtac
    46441 ccacaacagt gcgccgatca cgttataagc agcgaaatga cggtacgaca tgtggcccat
    46501 tcccgcaaca aacggggcga acgttctgac gatcggcaca aaacgggcga gaataatcgt
    46561 tttgccgcca tgtttctcat aaaactgatg ggttttgtcg agataactgc gacggaaaat
    46621 tttcgaattg ggattactga ataacttttc accgaacaac cgcccgatag tgtaattgac
    46681 cgcgtcaccc acaatcgcgg cgatcagcat cagcactacc atcatatgga cgttgagatc
    46741 gttggtttcc agtgatgcca acgctccggc aacaaacagc aatgaatcac ccggtaaaaa
    46801 cggcattacc accagaccgg tttcacagaa caaaattaag aacaaaatgg cataaaccca
    46861 gacgccgtac tccgcgacta gctctgccag atgcacgtca atgtgcagga taaaatcgat
    46921 gaggaaataa atcaggtcca tatttgccta tgccttgtac tcgtcatctt tcaggctgta
    46981 actacgttgg ctacagcctg aaatgttccg agcattattc tcgttagtcc gccagaaata
    47041 gcgggcccat tggcggtttt ggaagatcat accggtcagg gtaatccacc gcgaccagat
    47101 acagcccttc cgcttttgcc gttgctgccg ccagcgttct gtcctttgcc gccagcaact
    47161 ctgctatcca gctctccggc tggttgtggg caccgacttc catcaggctg ccgacaatat
    47221 tcctgaccat atgatgtaca aaggcattcg ctttgatatc aaccaccaca taaggaccgt
    47281 gacgcgtgac attaatatgc ataacgttgc gccacggggt tcgggactgg cactgcaccg
    47341 cacggaacga ggtgaaatca ttctcgccca gcaagcattg cgcagcccga tgcatccgtt
    47401 cagcgtccag cggttcgtaa aaatgggtta cccctttact cagtaccgcc gggcgcagcc
    47461 gatgattgta gatgatgtag cgataacggc gagccgtggc gctaaatcgg gcatgaaaat
    47521 catcaggtac agctttaacc caacgcacgg cgatgtcacc aggtaaattc gcatttacgc
    47581 ccaaggtcca cgccgcgtct ttgcgctgcg cggttgtttc gaaatgcacc acttgcccgg
    47641 taccgtgtac ccctgcgtca gtacgcccgg cgcagaagac ggtgatgggt tcgttcgcca
    47701 cctgggagag cgccttttcc agcttctcct gcacactgcg gacttcattc tgccgttgcc
    47761 agccgtaata cttactgccg tcgtactcaa tgcccagcgc aattttataa actggcagtt
    47821 gttgctggtc ggacattagt acagatactc ctgcaccagt ttctcggcga tttttactgc
    47881 catcagcgcg ccgccaaagc gaacgttatc ggccaccgac cagagctgga cttgctccgg
    47941 cataccgtag tcattacgca cgcagccaac agaaagatgc ggcgtacccg aagcatcccc
    48001 tacctgagtc gggaattcgt tctcttcaga gagcacaata tcttcgccct gagcaaacgc
    48061 atcacgcgct tcttctgccg ccagcggacg cagtgcttca aagttgacca tctgggcatg
    48121 accgtagaat accggtgcct ggacgacgct tgccgaaatc atcagccctt cgtcctgcag
    48181 gattttgcgt acttcgtcaa cgatacgacg ttcttcacgc acgctacctt cgctatccgg
    48241 caacaacggc agcatattga acgccagttg acgaccgaag aaatcttctt cgtcaatcgg
    48301 aatgccgttg agcaatttcg cactctgccc cgctaacgca tcgaccgctt ttttgccctg
    48361 ggcggaggct gaaatcaggc tggtaacact gatacgcgac aaaccgccct gatcgattaa
    48421 cggtttcagt gccgccagca gctggctggt cagactgtct ggtacggcga tgacattccg
    48481 gttgcggtaa tcggtcagta caaacgggtt tacttccggc accaccagcg gtacgtcggg
    48541 ttcgagagca aacagtccgc tgctgtcgat caccaggcaa cctgagttgg tcgcttcttc
    48601 aacccaggca gcggtagctt ctttgcctgc gacaaaaaat gccagctgcg cctgcgtcca
    48661 gtcgaattca gcggcatcct gcacggtgat tgttttacca ccaaagcgca gttgttcgcc
    48721 tgcgctttcg ttacgtgcca gtgcataaat ttccccaacc gggaactgac gttcagccag
    48781 cgtttcaagc agggcttcgc ccatagcgcc agttgcgccc aggacggcaa tgttccagcc
    48841 ttcagacatg gtggtttact ccagaaatag caaagctccc tgccaatgtt acagcagaga
    48901 gcatgaagaa gagattaacg tgccggatga tgaacggcgt taaaacccag tttacacagc
    48961 aatgatgccg cactggcgtc atcacaaatt acatacagag atgaccattc acggcgctca
    49021 agatagtttt tgcgcagttt atcgaactca cccggtatcc cggcgacttt acgcagcggt
    49081 gcgtcatcgc ggcgcacatc atacaccaaa tgcaccagcc ttttcagcgt cggttgatcg
    49141 agcggaccat gcagcgtaat gcggccaaac tcaggcgcgg gcagcaacgt atccagcgca
    49201 acgtgttgtt catgcccaat aaacttgcta taggcttcaa acacttgcgt cgtgccacgc
    49261 gctttaccct ccagggtata gcctgcgata tgcggcgtgc cgatatccac ttttgtcagc
    49321 aattccacat taagttccgg ctcgccttcc cagacatcca gcaccacgct taacttctgg
    49381 ccttcattca ggcaggtcag cagcgcggta ttatcgacga ctgcgccacg gcaggcgtta
    49441 atcagaatcg ctccgggctt caggctacgg atcagttttt catccgccag atgtagcgtt
    49501 ttgtacggac catctttaaa gagtggcgta tggaaagtca gaatatccgc gcgctggact
    49561 aactcatcca gcgagcggaa atcaccctca tccccacggt cggcgcgagg cggatcgcaa
    49621 agtaaggttt taatccctaa cgcttccagt cgcgcctgca atcgacgtcc aacgttacca
    49681 acgcccacga tccccaccgt acggtcgtgc agtgaaaatc catcgcgttc ggcaagcatc
    49741 agcagggagg agaaaacata ttccaccacc gcaatcgcat tacagccagg tgcagcggaa
    49801 aaaccaattc ccgcctgctt caaccatgct tcatcgacat ggtcggtccc cgcagtggca
    49861 gttccaacaa atttaatggg ttttcctgcc agcaaagatt cattcacttt cgtgaccgaa
    49921 cgcaccatca gcgcatccgc gtctgccagt tgagcgacgg ggattggacg cccgggaacc
    49981 gcgatcacct cacccaaacg gctaaataag tcgcgggcat aaggcatatt ttcatcaaca
    50041 aggattttca cgtttgtgtt acctgtatga gacgagagtt aaccggacaa gtgtgccata
    50101 atctcgcggc caggcatact tgcgaagatt tcaggtataa ggatacgtaa tgatacaacc
    50161 tatttccggc cctcctcctg ggcagccacc aggtcaggga gataacctgc cgtctggcgc
    50221 gggcaatcag cctttatcca gtcagcaacg tacttcgctg gaaagcttaa tgacgaaagt
    50281 gacctcactg acgcaacaac aaagagcaga actgtgggcg ggtatcaggc acgatattgg
    50341 tctgtcggga gattcaccgc tgctttcgcg tcacttccct gccgctgagc ataatctggc
    50401 gcaacgtctg ctggccgcgc aaaaaagcca ttctgcccgc cagcttttag cgcaattagg
    50461 ggagtattta cgtctgggga ataatcgtca ggcggtcacg gattatatcc gtcataactt
    50521 tggtcagacg ccgctgaatc agctctcacc ggagcaatta aaaaccattc tcaccctgtt
    50581 gcaggaaggg aagatggtta ttccgcaacc acagcagcgc gaggcgaccg accgtccttt
    50641 attaccggcg gagcacaatg cgctaaaaca gctggtgacc aaacttgcgg cggcaacggg
    50701 ggaacccagc aaacagatct ggcaatcgat gctggaactt tccggggtga aagatggcga
    50761 gttaattcca gcgaaactgt ttaaccatct ggtgacctgg ctacaggcgc gtcagacgct
    50821 aagccagcaa aatacgccga cgctggaatc actacagatg gcgctaaaac aacctttaga
    50881 tgccagtgaa ctggcggcgt tatcggcata tatccagcaa aaatatggcc tttctgcgca
    50941 atcatcgctt tcttctgccc aggccgagga tattcttaat cagctttatc aacggcgggt
    51001 taaagggatt gatccgcgtg atatgcaacc gctgcttaat ccttttccac cgatgatgga
    51061 cacgttgcaa aatatgccaa cgcgtcccgc gctgtggata ctgttagtcg cgattatcct
    51121 gatgctggtc tggctggttc gttaaccccg acgaaatgac agtatcgtga caataatacc
    51181 cagcaccgca gagatcgccc cggcaagaaa taccgaagag taaccaaacg tggtcgccag
    51241 cattcccgcc agcggcccgg agacgccgag ggcgatatcc tgaaacgcgg cgtaaccgcc
    51301 cagtgcggtg ccgcgaactt gtgaggggac gcgtttaacc acctccacgc ccagcgcagg
    51361 aaagataagc gaacatccgg ctccggttaa cgccgcgccc gctaatgcga cccatgcacc
    51421 tggggcttgc cagagcagca acaagcccac cgtttctaca agcagagaga caatcgccac
    51481 tttcacgccg ccaaaacggt ccggcatcca gccaaacatg acgcgcatca cgacaaatgc
    51541 gccgccaaac gcggtaagag taaagcccgc catcgcccat cctttgctgg caaagtagag
    51601 cgaaacgaaa gtcccgataa ccgcaaaacc aacgccttgt agtgccagcc ctaaccctgg
    51661 tttccagata agcccgacaa cgctccacag cgatggacgt tctcccgcca gggccggtac
    51721 tttgcgcact gtgccgttac aggcccacgc cagtaagggt aataccattg tggtgatcgc
    51781 cagtgcggca aaaccgtaat ggctatgaat caacaggcca agcggcgcac caacagcgag
    51841 ggcaccgtaa atcgccattc cgttccatga catcactttg ccagagtgtt ttggccctac
    51901 gatgcctaac ccccaggtca gagcgcctgt cagtaactgg ctttcaccaa acccaagaat
    51961 caaacgcccg atgaccaaca gggcaaattt gaacggtgcg gagacaggca aaatcgccgc
    52021 cagcagcaac gcgccgccag ccagaccaca agctaacatc ccctgaagcg ccgaacgttt
    52081 tgcgccatat tgatcggcca gtcgcccggc gtaaccacgc gtcagcaccg tagccagaaa
    52141 ctgaatcccg acggcaatgc cgaccatggt attgccatag cccagatcat gatgaacaaa
    52201 cagcgggata accggcaacg gcaaccctac ggtcatgtag gtgagaaaaa ccgcaaaagc
    52261 gatgcggaag agcgaaaaac tggcagaaga tcgtgtttcg gtttggctta cagcagtcat
    52321 gcattactcc agaatgcagc gcaaggcgag gagtatcccc gtctcatctc tctggtttca
    52381 gggttacggt gcgttggcag gatttaacgc gtacgtcttt tcagaaggaa atcgacaaag
    52441 cgggaagttt gcctagaact ggtggcgatt gtcaatgatg tgaaaaaggg aaccatcagg
    52501 ttcccttttg cgttagtgcc ggaggccgca ttggcgcgta acgtcggatg cgacgctggc
    52561 gcgtcttatc cgacctactc cggcctactg cgaattaatc tttcagcttg cgcattacca
    52621 gcgtggcgtt ggtgccgccg aagccgaagc tgttagacat aacagtggtc agttcgcgat
    52681 cggtcgtttc ggtcacgatg ttcagacccg cagcctgctc gtccagctct tcaatgttga
    52741 tgctcggggc gataaagccg tgttccagca tcagcagaga gtagatagct tcctgcacac
    52801 ccgcagcgcc cagtgagtga ccggtcatgg ctttggttgc agaaatcgcc gggctcttat
    52861 cgccgaacac ttcacggata gctgccagct ctttcacgtc gccaaccgga gtcgaagtac
    52921 cgtgagagtt caggtaatcg attggggtat caacgccatg catcgccatc tgcatacaac
    52981 gtactgcgcc ttcgccagac ggagcaacca tgtctgcacc atcagaggtt gcgccgtagc
    53041 caacgatttc agcatagatg taagcaccac gcgccagcgc gtgttccagc tcttcaacca
    53101 ctaccatacc gccgccgcca gcgataacga aaccgtcacg gtgagcgtcg taagtacggg
    53161 aggctttttc cggggtgtcg ttgtatttag tagacagcgc acccatcgcg tcgaattcgc
    53221 aggccatttc ccagcacagc tcttcgccgc cgccagcaaa cacgatgtcc tgtttgccca
    53281 gttggatctg ctctactgcg ttaccgatac agtgtgcgga agtcgcacac gcggagctga
    53341 tggagtagtt aacgccatga attttaaacg gcgtggcgag acaggcagaa acgccggatg
    53401 ccatcgcttt agtgaccaca tacgggccaa ccgctttcag gccgcgcggg ccgcgcattg
    53461 cgtcagcgcc gaacacctgg aaacgcgggg aaccgccgcc ggaacctgca atcaggccaa
    53521 cgcgcgggtt attctggtaa gcttccggag agaggcccgc atcagcgatt gcctgctcca
    53581 tagaaaggaa tgcataaatg gatgcgtcgc tcataaagcg cacaactttg cggtcaatga
    53641 ggccagtgat atccagtttt atgttgcccc agacgtggct acgcatgccg gaatccttca
    53701 gctcctgaga gaaagtgatc cctgaacgtc cttcacgcag agatgccagg acttcctgct
    53761 ggttattacc gatgctggaa acaatgccca ggccagtaat cactgcacgt ttcattcaat
    53821 acctctgtaa gtcgcacata gagtaagttt cgaatgcaca atagcgtaca cttgtacgcc
    53881 gaacaagtcc gatcagccat ttaatagaga aatttgcgca gccttacaca catcgctaag
    53941 atcgagccac cgcctgtaag acgagtaact tacgtgaaac actactccat acaacctgcc
    54001 aacctcgaat ttaatgctga gggtacacct gtttcccgag attttgacga tgtctatttt
    54061 tccaacgata acgggctgga agagacgcgt tatgtttttc tgggaggtaa ccaattagag
    54121 gtacgctttc ctgagcatcc acatcctctg tttgtggtag cagagagcgg cttcggcacc
    54181 ggattaaact tcctgacgct atggcaggca tttgatcagt ttcgcgaagc gcatccgcaa
    54241 gcgcaattac aacgcttaca tttcattagt tttgagaaat ttcccctcac ccgtgcggat
    54301 ttagccttag cgcatcaaca ctggccggaa ctggctccgt gggcagaaca acttcaggcg
    54361 cagtggccaa tgcccttgcc cggttgccat cgtttattgc tcgatgaagg ccgcgtgacg
    54421 ctggatttat ggtttggcga tattaacgaa ctgaccagcc aactggacga ttcgctaaat
    54481 caaaaagtag atgcctggtt tctggacggc tttgcgccag ctaaaaaccc ggatatgtgg
    54541 acgcaaaatc tgtttaacgc catggcaagg ttggcgcgtc caggcggcac gctggcgaca
    54601 tttacgtctg ccggttttgt ccgccgcggt ttgcaggaag ccggattcac gatgcaaaaa
    54661 cgtaagggct ttgggcgcaa acgggaaatg ctttgtgggg tgatggaaca gacattacca
    54721 ctcccctgct ccacgccgtg gtttaatcgt acgggcagca gcaaacggga agtagcgatt
    54781 atcggcggtg gtattgccag cgcgttgttg tcgctggcgt tattacggcg cggctggcag
    54841 gtagcgcttt attgcgcgga tgaagccccc gcgctgggtg cttccggcaa tcgccagggg
    54901 gcgctgtatc cgttattaag caaacacgat gaggcgctaa accgcttttt ctctaatgcg
    54961 tttacttttg ctcgtcggtt ttacgaccaa ttacccgtta aatttgatca tgactggtgc
    55021 ggcgtcacgc agttaggctg ggatgagaaa agccagcata aaatcgcaca gatgttgtca
    55081 atggatttac ccgcagaact ggctgtagcc gttgaggcaa atgcggttga acaaattacg
    55141 ggcgttgcga caaattgcag cggcattact tatccgcaag gtggttggct gtgcccagca
    55201 gaactgaccc gtaatgtgct ggaactggcg caacagcagg gtttgcagat ttattatcaa
    55261 tatcagttac agaatttatc ccgtaaggat gactgttggt tgttgaattt tgcaggagat
    55321 cagcaagcaa cacacagcgt agtggtactg gcgaacgggc atcaaatcag ccgtttcagc
    55381 caaacgtcga ctctcccggt gtattcggtt gccgggcagg tcagccatat tccgacaacg
    55441 ccggaactgg cagagctgaa gcaggtgctg tgctatgacg gttatctcac gccacaaaat
    55501 ccggcgaatc aacatcattg tattggtgcc agttatcatc gcggcagcga agatacggcg
    55561 tacagtgagg acgatcagca gcagaatcgc cagcggttga ttgattgttt cccgcaggca
    55621 cagtgggcaa aagaggttga tgtcagtgat aaagaggcgc gctgcggtgt gcgttgtgcc
    55681 acccgcgatc atctgccaat ggtaggcaat gttcccgatt atgaggcaac actcgtggaa
    55741 tatgcgtcgt tggcggagca aaaagatgag gcggtaagcg cgccggtttt tgacgatctc
    55801 tttatgtttg cggctttagg ttctcgcggt ttgtgttctg ccccgctgtg tgccgagatt
    55861 ctggcggcac agatgagcga cgaaccgatt ccgatggatg ccagtacgct ggcggcgtta
    55921 aacccgaatc ggttatgggt gcggaaattg ttgaagggta aagcggttaa ggcgcggtaa
    55981 tctgctctcg cagtgtttgc cggatgcggc gtgaacgcct tatccaacct acgtgtgacg
    56041 tgtaggcctg ataagacgcg ataagcgtcg catcaggcat ggtgctccag acgccgcaac
    56101 ttacagctgt gacgcctgct gaaacaggtt ttcccacata tcggtcacca gcgcctggtc
    56161 acgcggcgac aattcaccgg caccaatggc tttttccaga ctctggctaa cggtcgtatg
    56221 caccgcctga gcggagtggt catcaccact ttccagttct gcgatggcta atgtcaggtg
    56281 gccacgcaaa tacccactgg caaacaactc atcatcactg gcatggtcaa ccatgccgtc
    56341 gattaatgcc agaatgcgtg attcaaactc cgcgatcatc ttctttcctc attgtaaaac
    56401 gtggcgctgg cttcagttga gcgcttccgg ccacgggaac tgttccgccg taagttccgg
    56461 cgtgtgataa taattttgta gtgctttaat gaagcgtgcc gggcgttccg gaatgccatt
    56521 tgtcagataa tccatcacct gcgcatgtac ccgccgttga aacaccacac gatccggttc
    56581 gaaatcccct tccagattgt cgcaactgac gttaaacgga tatccagccg ccacgcagaa
    56641 taaccagtcg aacgcctgcg gcttcacttc aacatcttca aactggcttt gcgtctgggc
    56701 atcgcgtccg tccgggcaat accagtagcc gaaatcaacc agctcacggc gcgcttttcc
    56761 ggcaatacac cagtgcgaaa tctcatgaat tgcgctggca taaaagccat gagcaaagac
    56821 gattcggtta tacggtactt ccgcatcagc aggaagatag atcggttcgt cgtcgccttt
    56881 aatcagacgg gtattaaatt catcggcaaa gcagctatta aaaatttcaa tcaactgctc
    56941 gtagtggtgt gtactgttca ttagttcatc cccaaccagt ggaggatctc ctgtccgtgg
    57001 ctatcgtaaa gtaatttggc actcatcacc gccgagacga taacaatcat cgggcggatc
    57061 agcttttgtc ctttgctcaa caccaaccgt gaacccatgc gcgcgccgag gaactgcccc
    57121 accagcatga caaagcccgt cgcccaaatc actttgccgc cgagaataaa cagcagcaga
    57181 ccgccaatgt ttgacgttgc gttgagtaat ttggcgtgag ccgtggcttt ggcgaggttg
    57241 aatccgcaca gcataacgaa ggccagcgcg taaaacgatc cggcagccgg gccaaagaat
    57301 ccgtcataaa aaccgacgca gccaccggca attaacgcga agggtaaacc gtacatccgg
    57361 cgctggcgat cttcttcacc cagctttggc atcagcaaga aatagaggcc gatacaaatc
    57421 accagaatgg gcaaaatctg ccgtaagaca tcagcctgaa cgtactgtac caacagtgcg
    57481 ccgctcattg agccgacaaa ggtcatggca atattgagtt tctgatcgct taaactaacc
    57541 actttgcggc gaataaagta gatagtggcg gaaatagagc cgccgcaggc ttgcagttta
    57601 ttggttgcca gcgcgttagc gggagacatt cccgcagcca tcaatgccgg aatggtgagt
    57661 aacccaccgc caccggcaat cgagtcgata aatcccgcca gcatggcgac aaaaaagaga
    57721 actcccagca acagcggaga aaccataaac aggctattaa acgtttccat tagatcacgt
    57781 gctcatccag tagcgcctgg caggaaggcg gcaacggagg cggtgtcttc ttctcaggct
    57841 ttgttgttcc cggttttgga ggttcaaacc agctttgcag ttctgccccg caaccatcgc
    57901 ctgatggcgg taaaggttga tcttcacact ccagactatc ggcagggcaa cgtaatcgta
    57961 catgcatatg cgcgcgatgc tggaaccagg gtcgcacttt gcgcaaccag tcgcgatcgg
    58021 tgcccgcatc aaggcaaagt tgttgtttaa tcgccggatt aacaaaaatg cgcgtgacgt
    58081 ctttgtcctg ggcggcgagt ttgatcaagc tgaaaatttc tggcttccac agcgtggaga
    58141 caacgtgttt accgtcgcgg gaaactaagt ccagtgcttg cgggcgcaag agctgcgcgg
    58201 aggtccagcg agttttcggc agttgcagaa agatatcgac atccagtccg gtctggtggc
    58261 tggcatgacc gccgttgaaa cgcccaccag cgggcatccc catatcgcca atcagcaccg
    58321 tacccatgcc cagattgctc acctggctac tcagacgctg gataaacatc accagatccg
    58381 ggtgaccgaa atagcgacgc tgatcggtac gcatgacctg ataatgttcg gactgtatcg
    58441 gcaacgtgtc agcgccgaca atacagccat tagaaaaact gcctatcgat tgtgcgctac
    58501 ccggcacagg ttgggttatt ttttgccacg gcgtcgctgc caggctagcg ctactggcaa
    58561 gcagagccag cagcgcaatc gcggttttgt tcatttttta ccagcgtgga atatcagtct
    58621 tcacatcggc attttgcgcc cgctgccgta acaggtgatc cattaaaacg atcgccagca
    58681 tcgcttcagc gatcggcact gcacggatcc cgacacaggg atcgtgacgg cctttggtga
    58741 tcatctcaac ttcttcgcca aagcggttaa tagtgcgccc cggcacggta atgctggagg
    58801 tcggtttcag cgccatatga gcaatgattt gctgcccgct gctgataccg ccgaaaatgc
    58861 cgcccgcatg gttgctctgg aaaccctctt tggtgatttc gtcgcggttc tggctgccgc
    58921 gcagcgccac tacgtcaaaa ccgtcgccaa tttccacgcc tttcaccgcg ttgatgctca
    58981 tcagcgcatg ggcgatgtcg gcatccaggc ggtcaaagac cggctcgcca agtccggcgg
    59041 ggacaccact ggcaacaacg gtgactttcg cgccgatgga gtcgccctct tttttcagcg
    59101 cgcgcatcag ttcatctaac gcgtcgattt tgtcggggtc cgggcagaag aacggatttt
    59161 gttcaaccag cgaccagtct ttgatttcca gcggaatgtc gcccatctgg gtcaggcagc
    59221 cgcggatttc aataccaaat ttctcggcga gatatttttt ggcaatcgcc cctgccgcca
    59281 cgcgcatggc ggtttcgcgg gcggaagaac gtccaccgcc gcgataatcg cgcagaccgt
    59341 atttttgttc gtaggtgtaa tcagcatggc ctggacggaa gacgtcttta attgcgctgt
    59401 aatcctgaga acgctggtcg gtattttcga tcaacaagcc aatgctggtg ccagtagtaa
    59461 cgccttcaaa aacaccggag agtattttga cctgatccgg ctcgcggcgc tgggtggtat
    59521 agcgcgatgt cccagggcga cgacgatcaa ggtcatgttg caggtccgct tccgtcagcg
    59581 gaatgcctgg cggaacacca tcgacgatgc agccgagcgc cagcccgtgc gattcgccga
    59641 aggtggttac gcgaaagagt tgtccaattg tgtttccagc catcacggct ccgttattgt
    59701 tgtgtttgcg tgtttactta atctttataa atcgcgaaat gttctcgtgc ggcaagaagc
    59761 tgctctttag tgagcataaa cacaccatcg ccgccgttat caaactccag ccaggtgaac
    59821 ggaacatcgg gatattgttc cataagatgt accatgctgt tgcccacttc acaaatcaac
    59881 acgccatcat cagcaaggta atctgccgcg ttaccgagaa tgcgacgcgt cagtttcagg
    59941 ccgtcggtgc cagatgccag gcccagttcc ggctcgtgac ggtattcgtt tggcaggtcg
    60001 gacatatctt ccgcgtcgac atacggcggg ttagtgacaa tcagatcgta ttgcactttc
    60061 ggcaaatcac ggaacagatc ggaacgaatc ggaatgacgt tgtggatcag accgtgttct
    60121 tcgatgttct gttcggcaac cgccagcgcg tcgggagaga tatctaccgc gtcgacttcc
    60181 gcttccggga aggcataagc acaggcaatg gcgatgcagc cgctaccggt acacatatct
    60241 aaaatatgct gcggttgctt gctgatgagt ccggcaaatt tattgttgat cagttcacca
    60301 atcggcgagc gcggcaccag cacgcgttca tcgacgtaaa attcatgacc gcagaaccag
    60361 gctttgttgg tcaggtaagc caccggaatg cgctcgttga cgcggcggat cacgcgttca
    60421 acaatacggt gtttttcgct ggaggtcaga cgcgcggtgc gcatatcttc cgggatatcc
    60481 agcggcaggt agagcgatgg taacaccagc tgtacggctt catcccacgg gttatcggtg
    60541 ccgtgaccgt accagatatt tgccgcgctg aagcggctta ccgaccagcg caacatgtcc
    60601 tgaatggttt gcagctcatt tactgcttca tcaacgaaaa ttttatccac gtattcctcc
    60661 agggcatgat cgcaataatt tcggcggcta gtttgccatg aagatgacga taaatcagca
    60721 ttcacgcgcg gtgagtgagg aaaaatacgt ttaaaacgat cgattgtgct acgagtcggg
    60781 taaactgtag gaaaattaga aatagagaca gataaatgaa aaagaaaaca acacttagcg
    60841 aggaggacca ggctctgttt cgccagttga tggcggggac tcgcaagatt aagcaggaca
    60901 cgattgtcca ccgaccgcag cgtaaaaaaa tcagcgaagt gccggtgaaa cgcttgatcc
    60961 aggagcaggc tgatgccagc cattatttct ccgatgagtt tcagccgtta ttaaataccg
    61021 aaggtccggt gaaatatgtt cgcccggatg tcagccattt tgaggcgaag aaactgcgcc
    61081 gcggcgatta ttcaccggaa ttgtttttgg atttacacgg tctgacgcag ctgcaggcca
    61141 agcaggaact gggcgcgttg attgccgcct gtcgccgcga acatgtgttt tgtgcctgcg
    61201 tgatgcatgg gcacgggaag catattttga agcaacaaac accgctgtgg ctggcacaac
    61261 atccgcatgt gatggcattt caccaggcac cgaaagagta cggcggtgat gcggctttgt
    61321 tggtgttgat tgaagtggaa gagtggttgc cgccggagtt gccctgaatg ctggcagcaa
    61381 atgccggagc agcgtaaacg ccttatccgg cctacaaata tgtgataatt caataaattg
    61441 caattcactt cgtaggcctg ataagacgca tcagagcaat actacgaact tcagttcatt
    61501 caatacatac tgaaaacgac acgtcgaatc aatcggttgc ccactactgg tattcacatt
    61561 caccacaata tcgtgtagta ctttatccgt taccggatga cgagttggcg taaccgttac
    61621 cgttgattta tcacaaccgt aagaggtaaa ggttaacaat tctgataccg cgcggcagta
    61681 tgctcctctg ccatcattat cagaaaaatt actgccatca cagagtctgc cgccttccat
    61741 caggtaggtc gaaagcgtgg tggtcactgt tgaccccgtg gactgaattc gtaacgcctt
    61801 attatggctg acataatttt cactccagga gatatcgtat gaagcgaccg gtatcgttgg
    61861 gcatattgtg ttatgcactg taggggccgt atagcttcca aatccgggta aatctgtata
    61921 ggaataaaag gatgcgatcc aagggcaacc ataatcatca gatgcaacgg taattttaaa
    61981 cgtcatatat gcacaacgaa aaattttcgt atctatgcca gggcaatttg agttattaat
    62041 tgtatcaaag tcagtcagta ccaaagttgg agctgaaccc gacccagacc aggaaataac
    62101 ccgcaacgta tcgcctttat acgcaaaaga atcatatttc ccatatgcaa cctcctttgg
    62161 cgttatcacc gtaacattga aaacactgct cgctacagca ccggttacct tgtccatggc
    62221 ataataaact ctcacactgt caaaactcgc actcataagt ttatctccag cataactctt
    62281 agatgcgccg acaacactca gaacgagcag cattaggctc gcgaaaatta tggtccattt
    62341 tttcatttca gaaatactca aaggttgccg tcactgtggc actaaagtct ccggacgtta
    62401 catctcgccc gctctttgcc tgtaaccagg tattaaaatt taggctgtta tttccctgat
    62461 tcagcgggag cgtgacgcct gttgtattgt tgattggcac cgatgtgcct gccgcagttt
    62521 caataccaat cccagcgccg gatgctgatg acgaggagtc aaaggccaga aagccgggta
    62581 gagcactatc ttctgttcct gtcaacgtta ctttgacatt aaacaatgta gaactatgac
    62641 aatctttcaa ttgaaacacc aatggcactc gctctgactg cccaaggttc attaaatcgc
    62701 tggcggcaat ggcaggaaaa tgtacttctg ccaactggct accattaatc actggcgtac
    62761 acgatttact gattaaattg ccggtaaagt gcaaattatc tcctgctatt gtggttccac
    62821 tgcccagcaa catcaggcta ctaataaaaa acaacgtacg ctttttcttc attggtaatc
    62881 tacctgtaac gtggcccagg cctcaaagtc cgcctctggc agaatgacac cactcttttt
    62941 tactggcaca gccgttaaca ccggtaaatc ggtttcattc accacaagtg gcgtattgat
    63001 cgtaaaactc tgccctgcct gtttgagctg aatgcctaaa ccagtgatat tgctgcttac
    63061 tgcagcaata tcaaaatcgc ttggtgtgcc actccagctc aaggtcattt ccattaatgg
    63121 gtcgcgaacc gccgtatcgc aggtaatgga atacggcacg gtttgctcat aattgctgcc
    63181 attaatgtca tcaataagca catcctgaaa agttatttca atggtttgag cgttgctaat
    63241 cgtgcagtta ggcggctgca ctaaataacc gtgaaaagag acgtttgaga gtgccatcga
    63301 gcaaaagccc cacagcaaaa taagcgagat ccgtttcatt ggtaatccac cacaattgtg
    63361 gcactggcgt taaattcagc tgccgctaac gttgtaccac tctgtttcac cggcacagct
    63421 tccagaacgg gaactcccga ggaaaaatta aatggcaacc atgagccaga ggttaagtcg
    63481 agtattgtgt gatcactact tttctgaacc cgaataccaa acccaggaat acccgtgctg
    63541 ataactgttt caccgttaat cacgaccgtt gtcgcctgca tctgtagacg caggtcatcc
    63601 attgccagat tgttacaaac aagcgagtag tcaatgggac gtttataatc aaccctattg
    63661 atcttattaa tgaatacatt accaaactca actgccgcac catttaccgt acaaggagga
    63721 ggggcatcga caagcacccg taaattcata cttactgact tactcgctgc aactgcaaag
    63781 ccgctaccaa cacaacatat cagtaaccca gttaccgccc agacagaaca agatcctttc
    63841 atactggctc tcccggcctc agcctttgcg gctctgctct tcatcgacct agcaactgtt
    63901 accgttgcaa gtgaaaaaga gcggcatccg agcgccatag tcgttgacat aggtcagtac
    63961 cggcacgcta cccatcttca cattcagcgg tgcataggtt ttcggcgaca tcactattgg
    64021 cgagaaaccg gctgccggat tgccattttt ctgtgtgctg gcattactga aaatgacgta
    64081 atacgacgtc gggttattga cggtaaattc ctgaccgttg cgggttaaag taaccttata
    64141 ttgctagggg tttttcatgc tgacgttctc cagcgccttc ggtcgccaga acaacttaat
    64201 acgcgtttgt agtgctattt gcagcgtgtt ggctttgttt gattttggcg ggatctcacg
    64261 cacgttgaag tagaacaggc tttcacgatc ggcaggcaat ttgttgatat ccggcattcc
    64321 ctgcactttt acctgcccgt tcatcattga atcgatgcgt tgcaccggtg gcagaacagt
    64381 taacggtgaa ctgatcttgt tacccttttc atcttcgatc cagctttggg ccagataagg
    64441 cagtttcgga tcgttattgc gcaacgtcac gctgattgat ttgtcgcttt cattaaatat
    64501 caaacgggta cgatctggcg ttacagaagc ctgggcagac aaactggcag cgctaagcag
    64561 taacgctagc gtcgtagcac ccaatttggc agagcaaagt aaatcagaca tggatcattc
    64621 cttaatcacg ttattggtta gctgaaacag aaacaggggg attggtgggt gtcacctgtt
    64681 gcgtctgctc ctgaatgaca ggctttatgt catcaggaac aaccggcgcg acgttgcctt
    64741 tatgctggca tggcagtaac aagccgttaa acagatcggc aggcagcggg tccggcaggt
    64801 tgatatcgca atgcgcaaca ccactccaga acacactcat gtgttcgcca ggtttcaccc
    64861 cagccagata aacattgccg tcatcatcga caaggcccac tgtctgctcg ttatcatttt
    64921 ttacttctgc gccaaacgga ggatggctgc cgtcttgtaa acgcagcacc gccatcgctt
    64981 tttgaccact gatgacggca aatttgcggt agccaatcgc cccttcagtt agcgtggctt
    65041 gtaccaccga ctgggttgct tcggcgtttt ccggcagttt gttgaggtcg atatacgcct
    65101 gattgcggta atagttattg acgtcagaaa cgacggcttt accaaacata ttggtataaa
    65161 cagcagcacc gttaccttct accggaacat cggcaacgcc atcggcatca atcaacaagc
    65221 gtgtcccgcc catattctgg gtacggtgaa gtgcgccacc gtgggcagta agcgttgcgc
    65281 cgccctgtaa cgacaagccc gcagaggtgt attgcccttc atggtagttc gcactgaggt
    65341 caacctgcgc cagcgaacca tcatggctgt aatagccgtc aacgctggtg tgtttgtcac
    65401 tggtgccgac gttcaactga tagtgagtcg cgtcatcgac acggctgaaa taaccgacct
    65461 gactgctgtc cgtcccactg ccatagtacc gttatagcta acggtactgt tgtcgcccca
    65521 cggcatactg agcgaaatgt acatgccttt gtcggcctgg ttgtcatact cgtaacggta
    65581 gccagtcatc gagatgctga cattacgaat actgcccata ttgaaatagt gcgagagcat
    65641 gatgttgtag tttgtctgct cctcgcgatc ccagtaggta tggcgggtat agttgagata
    65701 aaccgaaaca cccgcatcgc ggaagttctg gttataagtg gcggtgtaca tctctttgtc
    65761 gttgcccgtg cgtaccattc cgctgtcact ggcatccaga tactcgctca tggtcataaa
    65821 gttctcttcc gagaagcgat atccagcaaa agtaacgcgg ctgttgagct ggtcaaagtc
    65881 tttggaataa ctcacacgga aggagttacc gtccagcgaa cctttgccat aagcggtgtc
    65941 tttatccagt ttggtgtgcg agtgagtaac atcaaacgcg accgcgccga atgtagacaa
    66001 atcgcgaccg acaccaagcg ccgcagactg atagttttca tctcccagtg cgccgccata
    66061 taacgaccag ccgttagcga tcccccagga agcttcagca ccagaaaaaa attcaccctc
    66121 gacatggtgt ccccactctt gcggacggcc catcatgatc ttatagcgaa cctgacctgg
    66181 acgagtgagg tatggcatcg aggcggtgct gatgtcatat tcctgcacct ggccgttctg
    66241 ttcttcaatg cgaatatgca acgtaccgga gacagaatca ccaagatcct gaatacgaaa
    66301 cggtccagcc ggaacctgcg tttcgtaaat cacacgcccc atctggctga cggtcacttt
    66361 tgctgtggtg tgtgccacgc cggaaatgtc tggcgcgtag ccgcgcagat tgggaggcaa
    66421 catttgatcg tcagtactga cactgccacc aacatagtta aaaccatcaa aaatatcgga
    66481 tctgaggtaa tcctcgccca gcgccagttt ggcttttaat gatggtaacg cccgcctggc
    66541 atagtagcga ctccactccc attttttttg agtttcatcg ccgctgaatt catcgtcatc
    66601 attactgcga gtatgttgat agttagtctg ccagtcagca cgcatacgcc acggccctag
    66661 gttaacccca accgtcccgt tgccgctgat ctcgttacta tcatcaccgc cattttcttc
    66721 gtgccgggtt tgtgcgttga tgctgtagtc cgcgacgatc ccggagatgc cgtcatccca
    66781 acgtgaaggc ggatcccagt cgggataagt atattcgagg taagcctgtg gcagtgaaat
    66841 gactaatgcg gactggctta aatcagcctt aatttccatg ccttccagtt gaccggattt
    66901 caggcattta gcatcgtggc tccattgcag atttttcgcc acgtcttctt ttaaaccaaa
    66961 ctgcgctacc agttccggtg tcagacaagc atagcttttg ctcgcgtcat cttcaccagc
    67021 ataccagtaa atatcgtact cttccgccaa tggctgttta tttagttgaa cctgtaaatt
    67081 gtattttccg ggctcaacat atccctggct ggaaaaacgc ttcagatcaa tttttgtgtc
    67141 gccttttaat tccagaaaac gggaatcaaa ctgaatgtcg tcttcagccc agacactgct
    67201 ataactccct gacattgcca gcgcaatgca ccacggaaga atccgtaatc gaaaaagaga
    67261 atggtcaggc atacacgttt ctatccgttt ttattcttgt tcggtcaaaa tccatttaaa
    67321 tcccccatag cagtgtcgct atgggggtat gccttacagg taagtaatct ggaaggtggt
    67381 atttgcttca aaattaccca gatctggcgc atcagctgcg ccgaccagcc aggctttaaa
    67441 gttcagtgtc tgcttcgctt tgcctttgtt ggtagaagtg tctttcacta ttttctggtc
    67501 aatacccatg ccgcttttgt aagaggtacc cagagagtca ccgatcgcca ggctaacatt
    67561 gttaaatgcc gcaccggtat cggtattgaa aatggtgtaa taattgccgc tatttgcaga
    67621 agaaacggta ccagtaaagg tagtggtcat tgtttcctga gtatcaaata cgcaatcttg
    67681 cagacgaatc tggaaacttt tcggcgtggt ggtgccgtta tttttcagtt tttcggcacc
    67741 gatatcaccc atatcaactt ccagtttgtg atcgtccgga acgatagaac acactgagtc
    67801 ttcaatggtg ccgtaaaaac gtgcagtacc gttgttaccc gcagcgattg ttgccgtaga
    67861 ggtgaacacg cccatcacca ttgccgcagc aatagctgtt ttaacaaact tactcattat
    67921 ttgtccttaa actataaata attaaaaata taaacattgc aatacattga ttcagtcaat
    67981 agccaatgtt tatacccagc cattgaatac ggcatgcgac ccaaaaagaa caggtcgtag
    68041 aaaattgtat tcattggtgt ggggccgcat agtgatgttt ccacacccct gatccaattt
    68101 aattttacta aagagtaaat gtagatttaa ttaatatatt gacagaagaa aggttatctt
    68161 aaggacatcc ttaaaaaata cggaataagt atttagaaaa aatagaaagt atatcaataa
    68221 tgaatatcaa cagcttaaaa aacaaacaat gaacatataa tgcgattggc atttatccac
    68281 ttttgacaat ataatatata aacactatag ccagaattta atcaagtttc aattacatcc
    68341 tgcctgatta ttcaactcta ttttctctat tatctgtatt aataaatgac actgagtgtc
    68401 aaacagacat tattcatttc aacatgtaac tttgggcaaa atcaagatac gcgcagaaga
    68461 ataatttccg aatgaatgta gaaagtgttt tatttataga taaggagagt cttcgatagt
    68521 agtgaggttg acagatatcc gctgcctaat gagcaattat gcccaatgaa tctacctcat
    68581 tgggcataat ttggaacaca tcagatagct tttgccatct tcagattaca cgggctcatc
    68641 tgccagttga atgtaccgtt tccgctttca tcaagcgtta cgctggcaat ggccgaggtg
    68701 gtaaacatag gcggcgtttc gcccggacat aactcggcaa ccagataccc gactaacggt
    68761 aagtgagaga tcaccagtac cgaagcaaca ccttcattgg tcaacgcctg caaataggca
    68821 ctgaccagac caacatcgcc gcagggcgtt aactccggca gaacttctgc actggaaggc
    68881 aggttcagac aatcccctac ttcttccagt gtttgctcgg ctcgcaggaa cgggctcacc
    68941 agaacgcgtt cgatttccac tttttgacct ttcagccagt tcgccatcag gcgagattcg
    69001 tcacaaccat tagtggtcag aggacgaacc gaatcactgg cggcatcgag ggctgcgtcg
    69061 ccgtgacgca tgataaaaac ttgcatattg caccgctttt gttaaccaga ttcgcccgtt
    69121 ttctttcatc tgaaaccaga gaaagaaaac ggtggccggc attgtgcctt atccattcac
    69181 cgaatgaaac gctgtttttt acctcaatgg cgtaagtata gtcaatatgc gtttacattt
    69241 tgaccaacac tccgccattc agcgcggatt catatagctt tgaccttctt attgcaggtc
    69301 agttgcagtt gttttccaaa aactttcccc acgcgcgccc atctcgacca aacgctcgca
    69361 aggggtaaaa cgggaaccat actgcgtggc cagtcgttgc attattgcaa ccacttcgcc
    69421 cgcgccgaga gaatcgatat agcggaacgg tccaccgaga aatggcggaa aaccaatgcc
    69481 aaataccgca ccaatatccc cgtcacgcac gctacggata acctgctcat caacacaacg
    69541 tactgcttca ttcagcatca acatcacaca ccgttcagca acctgcggtg cggagagtcg
    69601 cccctgccct tgtgcgccaa tgagcgggta aatggcagga tcgacctgtt ttttgctttt
    69661 acgccctttc tgaccataaa gatagaaacc ccgaccattt tttctgcctt tgcgatcgtc
    69721 gttcaaaatt gaagaaacaa catttgcagg cgcgctaaaa cgttctccat aagcggcttc
    69781 cagtacagga ataattttag tcccggtgtc gattcctacc tcatccaaaa gttggattgg
    69841 gcctaccgga aaaccaaatt tcactagcgc ggcatcaatg tgctctaccc gttcaccttc
    69901 ggtcagcatg cggatagctt cattaatgta aggcgctaag atgcgattga cgtaaaaccc
    69961 ggctttgtca cgcacgacaa tgggcgtttt gccctgtttt ttcgccagtt ttaccgtagt
    70021 ggcgatggtt tgcgccgatg tacccgcatg aggaataatc tctaccagcg gcattttttc
    70081 caccggactg aagaaatgca ggccaataac ttgctcaggt cgcgcggcgt gagcggcgat
    70141 atcaccaatc ggtaaagatg acgtgttcga agcaaaaatg gtatgagcgg cgcaattttg
    70201 ctccacttcc gccaccatct gttgttttaa ttcgagattt tcaaacaccg cttcaataat
    70261 cagatcgcga tgggcaaagc cgcgatagtc cgtcgttccg gagattaatg ccagctgttt
    70321 gtcacgttcg ctggctttga gatgacgacg gcgaactttg ccctccagct gatcccaact
    70381 gtacttcagc gcatgattta tgccctgcgg gttgatatct ttaattctga ccggaagccc
    70441 cgctttacaa gcagtgacat aagcaatacc gccgcccatc aagccaccac ctaaaattcc
    70501 cacgctgttt aatggcgcag gcggtgcatc actaccggga tctttcttca cgtccgtact
    70561 ggcaaaaaag atactacgca aagcttgcga ttgcggcgtc atcgccagtt cgccaaaggc
    70621 gcgagcttca gcgtcatagc cgctgctggt gccctgcgct aatcccgttt caacaacctc
    70681 caggatgcgt tctgtcgccg gataattgcc ttgagttttg tgttctgttt tcttgccgac
    70741 cattttgaac agcagcgcac gacctaacgg ccccgccaga atacgctcgc gtacaggtag
    70801 agggcgggaa gatgggcgat ccttcttcgc cagctcaaca gcggcttcca gcaaaatgga
    70861 gtgcggaaca acgtcatcca ccagccccag ctttaatgcc tgtttcgccc gaagttgttt
    70921 tccggtgaga atcatctcta atgctgtact gacgcctatc agacgcggta aacgctgggt
    70981 gccgcctgaa ccgggtaaca atccaagttg tacttcaggc aaaccgagca ccgttttagg
    71041 atcgtcagta caaacgcgac cgtggcacgc cagcgccagc tccagcccac cgcccaggca
    71101 agcgccatga atagccgcga taaccggaat gggcaaagca tgaatctccg ccatcaactg
    71161 ttggccctgc cgcgccagtg cttccgcttc ttgcgccgtt ttgcagttgc caatcatgtt
    71221 gatgtctgct ccggcgataa aattgtccgg tttcgcggag ataaacacca cgccgcgcaa
    71281 ctctttgttt tcacggagtt gcttaataat ggcgcgcacc tgcgaggcaa attccgcctt
    71341 cagggtattc attttctcat ccggtacgtc gatggtgatg atagcaatgt tgtccagacg
    71401 aacattaaga gtaaacgctg atgccatttc cattattccg cctccagaac cattgccgcg
    71461 ccaagcccac cggcagcaca ggcggtaact aaaccaaatc caccgccgcg acggcgaagt
    71521 tcatgcaatg tctgggtaat catccgcgcg ccggtcgccg cgaagggatg tccgtaagca
    71581 atcgaaccgc caagcacgtt aaatttgcta tcgtccactt cgccagtggc atgtgcacgc
    71641 cccagtactt cacgagcaaa acgttcacta cccagcaact gaatattcgc cagtgtctga
    71701 gctgcaaagg cttcgtgcat atcgatcaat gtcagttcgc tcatcgtcaa accggcacgt
    71761 tccagcgcca gcggtgttga ccaggctgga ccgagcaaca tgtcctgcca gacatcaatc
    71821 gcagtaaatg cgtagctgcg cagatacccc agcggcacca gccctaattc tttcgcccgg
    71881 gattcagtca tcaggatcac cgctgccgcg ccatcggtca gcggcgtact gtttgccgcc
    71941 gttaccgttc cgtgtttgcg atcaaacgcc gggcgcagct ttgcgtaatc ggcaagcgag
    72001 gaattaccgc gaatattgtt gtcttcgaca agcggttgtt tataaggagg gataaaggca
    72061 gtcatcacct cttctttgag ttttccgtct gaccatgcct gagcggcacg ctgatgcgaa
    72121 cggtgcgcta atgcatcttg ctgttctcgg gtgatgccgt aggttttcgc catttgttcg
    72181 gcggtgtcgc ccatccgcaa gccagtagaa tattctgcta ccgcaggcgg tacgggcatt
    72241 aagtcgcgca aacgcaggcg agagaagagt ttcaggcgct ggctcatggt tcgagctttg
    72301 ttgacatcaa ccagcacgtg cgccagtttt ttactgacgc caattggcaa tacggaagag
    72361 gaatctgccc caccggcaat ccccgctcgg atagttcccg ccatcaggct ttctgcgacg
    72421 tttgcaaccg cctggaaact ggtagcgcaa gcgcggctga cgctgtaagc atcggtatgc
    72481 acattcattc cggtaccgag aacaatttca cgcgcaatgt tgggggcttc aggcatttgt
    72541 acgacctgac caaagaccag ctgttcaatc acttcagcgg ggatctcgct gcgtgccagc
    72601 agttcgccta ccaccatctt ccctaaatca accgcgggaa tgccatgaaa agccgtcgcc
    72661 tggcgggcaa aaggcgtacg taaaccgcta acaatggcga tacgatcgcc ctggcgggta
    72721 accagtggta aaacctgacc cataacactc ccctgtaaaa aaataaataa agtggtctga
    72781 cctgatcata gtattaacca tttttttaca tttagccaag cggagaaaaa ggaaagtggg
    72841 agctatgaca cagtgaagac agaagaaaga aaagaaaacg cccctgccag caactgacag
    72901 gggcgtacgt acccgagagg aattaacgca gacccagctg gaagatcagc atttcagctt
    72961 cgcaggcaaa agtgaaatcg atatccaggc gcacaccgtc agactcttca gtgaaagtcg
    73021 gggagatttt gcacggttcg gattccacgc tacgggattt ttcagtcagc gccgccagcg
    73081 tttgttctgc ttctgcgcgg tttgcaaaca cgcggctgta agacgcggtg cagtcggagt
    73141 tgtccataat ggtgccaaca tccatacagc agcaaaccgg ggtttcatca gcactacatt
    73201 tactcatcgt tgatttcctc tgtatgtgca cccaaggtgc cagataaacg ttgtggatat
    73261 tttacgcttc cggaaagtgc tgctccagtt gttaattctg caaaatcgga taagtgaccg
    73321 aaatcacact taaaaatgat ctaaaacaaa attcacccga atccgcgagt gcgcaacctc
    73381 caaattttgc cagctggatc gcgtttctta gatcatattt gaaaaaagat ggaaacatac
    73441 ttgcaacatt ctagctggtc cgacctatac tctcgccact ggtctgattt ctaagatgta
    73501 cctcagaccc tacacttcgc gctcctgtta cagcacgtaa catagtttgt ataaaaataa
    73561 atcattgagg ttatggtcat gagccagaaa accctgttta caaagtctgc tctcgcagtc
    73621 gcagtggcac ttatctccac ccaggcctgg tcggcaggct ttcagttaaa cgaattttct
    73681 tcctctggcc tgggccgggc ttattcaggg gaaggcgcaa ttgccgatga tgcaggtaac
    73741 gttagccgta accccgcatt gattaccatg tttgaccgcc cgacattttc tgcgggtgcg
    73801 gtttatattg acccggatgt gaatatcagc ggaacgtctc cgtctggtcg tagcctgcaa
    73861 gccgataaca tcgcgcctac ggcatgggtt ccgaacatgc actttgttgc accgattaac
    73921 gaccaatttg gttggggcgc ttctattacc tctaactatg gtctggcaac agagtttaat
    73981 gatacttatg caggcggctc tgtcgggggt acaactgacc ttgaaaccat gaacctgaac
    74041 ttaagcggtg cgtatcgctt aaataatgca tggagctttg gtcttggttt caacgccatc
    74101 tacgctcgcg cgaaaattga acgtttcgca ggcgatctgg ggcagttggt tgctggtcag
    74161 attatgcaat ctcctgccgg gcaaactcct caagggcaag cattggcagc taccgccaac
    74221 ggtatcgaca gtaataccaa aatcgctcat ctgaacggta accagtgggg ctttggctgg
    74281 aacgccggaa tcctgtatga actggataaa aataaccgct atgcactgac ctaccgttct
    74341 gaagtgaaaa ttgacttcaa aggtaactac agcagcgatc ttaatcgtgc gtttaataac
    74401 tacggtttgc caattcctac cgcgacaggt ggcgcaacgc aatcgggtta tctgacgctg
    74461 aacctgcctg aaatgtggga agtgtcaggt tataaccgtg ttgatccaca gtgggcgatt
    74521 cactatagcc tggcttacac cagctggagt cagttccagc agctgaaagc gacctcaacc
    74581 agtggcgaca cgctgttcca gaaacatgaa ggctttaaag atgcttaccg catcgcgttg
    74641 ggtaccactt attactacga tgataactgg accttccgta ccggtatcgc ctttgatgac
    74701 agcccagttc cggctcagaa tcgttctatc tccattccgg accaggaccg tttctggctg
    74761 agtgcaggta cgacttacgc atttaataaa gatgcttcag tcgacgttgg tgtttcttat
    74821 atgcacggtc agagcgtgaa aattaacgaa ggcccatacc agttcgagtc tgaaggtaaa
    74881 gcctggctgt tcggtactaa ctttaactac gcgttctgat aacgcgttcg cctggataaa
    74941 gtcacctgct atgcaggtga ctttaactcc cccacttcac cgaaagtagt ttcctcattg
    75001 cttgcctcct gagttttgca aacagcctga tggcagcttg ccctttcaaa tcagtaagtg
    75061 gtatccacag acaaacctga agggaaatgc atattttcaa gcgttctgct cgtccttctc
    75121 aaagagttta cttttctgca tttccaggat actcaacccc cctggctatt gtgcgcttat
    75181 acactcaaat taaagatagg ttctaaataa atgagcgttt tttgatagtc tatttcatta
    75241 ggtaatatat atttgtaaca aatcaatcaa catagaataa aatcatgcta ccatctattt
    75301 caatcaacaa taccagcgca gcttacccag aatccatcaa tgaaaataac aatgatgaaa
    75361 ttaatggatt agtacaagag ttcaaaaacc tttttaatgg taaggaagga ataagcacct
    75421 gtattaaaca tctacttgag cttataaaaa acgccatacg agtaaacgac gatccttata
    75481 gatttaatat taataattcc tcagttactt atattgatat tggctccaat gatacagacc
    75541 atattactat tggtatcgac aaccaataga attacctgcg aactataaag acaaagaact
    75601 cgtccgtact atcattaatg acaacatagt tgagaagact catgatatca ataacaagga
    75661 aatgatcttc agcgcattaa aagaaatata tgatggagat cctggtttta tcttcgataa
    75721 gatatcacac aaactcagac atacggtaac ggaatttgat gagagcggga aaagcgaacc
    75781 aacggactta tttacctggt acggtaaaga taaaaaaggc gactctctcg ctattgtaat
    75841 taaaaataaa aacggaaatg attacttatc tctcggttac tacgatcagg acgactacca
    75901 cattcaaaga ggaattcgta ttaatggtga tagtctcacc caatattgta gtgaaaacgc
    75961 caggagtgct tcagcgtggt ttgaaagcag taaagctatc atggcagaat catttgcaac
    76021 tggttccgat catcaggttg taaacgagct caacggggaa agactgagag aacctaacga
    76081 agtttttaaa cgtttaggtc gagcaataag atataatttt caagtggacg atgcaaaatt
    76141 tagacgcgat aatgtaaaag aaataatttc tactttattc gctaacaaag ttgacgttga
    76201 ccatcctgaa aataaataca aagactttaa ggatctcgaa gataaagttg aaaaaagact
    76261 tcaaaattgc caggcgaaat atcaaaatga aattaatcaa ctatctgcac taggtgttaa
    76321 ttttgatgat atttaaaatt atttttccag ttttttgatt tttcaacgcc tgaatatcgg
    76381 aaatagtcag tgacaaac
//
LOCUS       DAJTYG010000005        68998 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTYG010000005 DAJTYG010000000
VERSION     DAJTYG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.005"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..190
                     /locus_tag="OO568_000384"
     CDS             <1..190
                     /locus_tag="OO568_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6639878.1"
                     /translation="VGIRIENLGGGVANIPFQNGILPVDPSGHGTVNMRAWPVNLVGG
                     ELETGKFQGTATITVMVR"
     gene            complement(233..1093)
                     /gene="rsmI"
                     /locus_tag="OO568_000385"
     CDS             complement(233..1093)
                     /gene="rsmI"
                     /locus_tag="OO568_000385"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6639879.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1158..3194
                     /gene="lpoA"
                     /locus_tag="OO568_000386"
     CDS             1158..3194
                     /gene="lpoA"
                     /locus_tag="OO568_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6639880.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNISTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLSVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3152..3547
                     /locus_tag="OO568_000387"
     CDS             3152..3547
                     /locus_tag="OO568_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6639881.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            3567..4157
                     /gene="diaA"
                     /locus_tag="OO568_000388"
     CDS             3567..4157
                     /gene="diaA"
                     /locus_tag="OO568_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6639882.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4167..4742
                     /gene="yraP"
                     /locus_tag="OO568_000389"
     CDS             4167..4742
                     /gene="yraP"
                     /locus_tag="OO568_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6639883.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(4856..5896)
                     /locus_tag="OO568_000390"
     CDS             complement(4856..5896)
                     /locus_tag="OO568_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6639884.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNGLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(5969..6604)
                     /locus_tag="OO568_000391"
     CDS             complement(5969..6604)
                     /locus_tag="OO568_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6639885.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6732..7250
                     /gene="yhbO"
                     /locus_tag="OO568_000392"
     CDS             6732..7250
                     /gene="yhbO"
                     /locus_tag="OO568_000392"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6639886.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTSAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7230..7673)
                     /locus_tag="OO568_000393"
     CDS             complement(7230..7673)
                     /locus_tag="OO568_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6639887.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7724..8026
                     /gene="yhbQ"
                     /locus_tag="OO568_000394"
     CDS             7724..8026
                     /gene="yhbQ"
                     /locus_tag="OO568_000394"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6639888.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8013..8516)
                     /locus_tag="OO568_000395"
     CDS             complement(8013..8516)
                     /locus_tag="OO568_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6639889.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8510..9034)
                     /locus_tag="OO568_000396"
     CDS             complement(8510..9034)
                     /locus_tag="OO568_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6639890.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9243..10238
                     /locus_tag="OO568_000397"
     CDS             9243..10238
                     /locus_tag="OO568_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6639891.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHISINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10247..11125
                     /locus_tag="OO568_000398"
     CDS             10247..11125
                     /locus_tag="OO568_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6639892.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11206..12213
                     /locus_tag="OO568_000399"
     CDS             11206..12213
                     /locus_tag="OO568_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6639893.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSIQQAFVKLRRGEIGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12331..13575)
                     /gene="mtr"
                     /locus_tag="OO568_000400"
     CDS             complement(12331..13575)
                     /gene="mtr"
                     /locus_tag="OO568_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6639894.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13729..15618)
                     /gene="deaD"
                     /locus_tag="OO568_000401"
     CDS             complement(13729..15618)
                     /gene="deaD"
                     /locus_tag="OO568_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6639895.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGELRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15611..15691)
                     /gene="yrbN"
                     /locus_tag="OO568_000402"
     CDS             complement(15611..15691)
                     /gene="yrbN"
                     /locus_tag="OO568_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6639896.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(15798..16682)
                     /gene="nlpI"
                     /locus_tag="OO568_000403"
     CDS             complement(15798..16682)
                     /gene="nlpI"
                     /locus_tag="OO568_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6639897.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(16791..18926)
                     /gene="pnp"
                     /locus_tag="OO568_000404"
     CDS             complement(16791..18926)
                     /gene="pnp"
                     /locus_tag="OO568_000404"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6639898.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTSVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19173..19442)
                     /gene="rpsO"
                     /locus_tag="OO568_000405"
     CDS             complement(19173..19442)
                     /gene="rpsO"
                     /locus_tag="OO568_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6639899.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19591..20535)
                     /gene="truB"
                     /locus_tag="OO568_000406"
     CDS             complement(19591..20535)
                     /gene="truB"
                     /locus_tag="OO568_000406"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6639900.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20535..20936)
                     /gene="rbfA"
                     /locus_tag="OO568_000407"
     CDS             complement(20535..20936)
                     /gene="rbfA"
                     /locus_tag="OO568_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6639901.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21100..23748)
                     /gene="infB"
                     /locus_tag="OO568_000408"
     CDS             complement(21100..23748)
                     /gene="infB"
                     /locus_tag="OO568_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6639902.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(23773..25260)
                     /gene="nusA"
                     /locus_tag="OO568_000409"
     CDS             complement(23773..25260)
                     /gene="nusA"
                     /locus_tag="OO568_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6639903.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25288..25740)
                     /gene="rimP"
                     /locus_tag="OO568_000410"
     CDS             complement(25288..25740)
                     /gene="rimP"
                     /locus_tag="OO568_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6639904.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(25947..26023)
                     /locus_tag="OO568_000411"
     tRNA            complement(25947..26023)
                     /locus_tag="OO568_000411"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25987..25989),aa:Met,seq:cat)
     gene            26371..27714
                     /gene="argG"
                     /locus_tag="OO568_000412"
     CDS             26371..27714
                     /gene="argG"
                     /locus_tag="OO568_000412"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6639905.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(27722..29329)
                     /locus_tag="OO568_000413"
                     /pseudo
     CDS             complement(27722..29329)
                     /locus_tag="OO568_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(29807..29893)
                     /locus_tag="OO568_000414"
     tRNA            complement(29807..29893)
                     /locus_tag="OO568_000414"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29857..29859),aa:Leu,seq:gag)
     gene            complement(29908..30240)
                     /gene="secG"
                     /locus_tag="OO568_000415"
     CDS             complement(29908..30240)
                     /gene="secG"
                     /locus_tag="OO568_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6639906.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30468..31805)
                     /gene="glmM"
                     /locus_tag="OO568_000416"
     CDS             complement(30468..31805)
                     /gene="glmM"
                     /locus_tag="OO568_000416"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6639907.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(31798..32646)
                     /gene="folP"
                     /locus_tag="OO568_000417"
     CDS             complement(31798..32646)
                     /gene="folP"
                     /locus_tag="OO568_000417"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6639908.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32736..34670)
                     /gene="ftsH"
                     /locus_tag="OO568_000418"
     CDS             complement(32736..34670)
                     /gene="ftsH"
                     /locus_tag="OO568_000418"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6639909.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(34770..35399)
                     /gene="rlmE"
                     /locus_tag="OO568_000419"
     CDS             complement(34770..35399)
                     /gene="rlmE"
                     /locus_tag="OO568_000419"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6639910.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35525..35818
                     /gene="yhbY"
                     /locus_tag="OO568_000420"
     CDS             35525..35818
                     /gene="yhbY"
                     /locus_tag="OO568_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6639911.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(35974..36450)
                     /gene="greA"
                     /locus_tag="OO568_000421"
     CDS             complement(35974..36450)
                     /gene="greA"
                     /locus_tag="OO568_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6639912.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36698..38131
                     /gene="dacB"
                     /locus_tag="OO568_000422"
     CDS             36698..38131
                     /gene="dacB"
                     /locus_tag="OO568_000422"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6639913.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGILKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFGVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38317..39489)
                     /gene="cgtA"
                     /locus_tag="OO568_000423"
     CDS             complement(38317..39489)
                     /gene="cgtA"
                     /locus_tag="OO568_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6639914.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39505..40470)
                     /locus_tag="OO568_000424"
     CDS             complement(39505..40470)
                     /locus_tag="OO568_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6639915.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLSPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40597..40854)
                     /gene="rpmA"
                     /locus_tag="OO568_000425"
     CDS             complement(40597..40854)
                     /gene="rpmA"
                     /locus_tag="OO568_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6639916.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40875..41186)
                     /gene="rplU"
                     /locus_tag="OO568_000426"
     CDS             complement(40875..41186)
                     /gene="rplU"
                     /locus_tag="OO568_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6639917.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41445..42416
                     /gene="ispB"
                     /locus_tag="OO568_000427"
     CDS             41445..42416
                     /gene="ispB"
                     /locus_tag="OO568_000427"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6639918.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42644..42922
                     /gene="sfsB"
                     /locus_tag="OO568_000428"
     CDS             42644..42922
                     /gene="sfsB"
                     /locus_tag="OO568_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6639919.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPRTHEFIDRTQLMRSYTKPKK"
     gene            complement(42970..44229)
                     /gene="murA"
                     /locus_tag="OO568_000429"
     CDS             complement(42970..44229)
                     /gene="murA"
                     /locus_tag="OO568_000429"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6639920.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(44284..44538)
                     /gene="ibaG"
                     /locus_tag="OO568_000430"
     CDS             complement(44284..44538)
                     /gene="ibaG"
                     /locus_tag="OO568_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6639921.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44698..44991)
                     /gene="mlaB"
                     /locus_tag="OO568_000431"
     CDS             complement(44698..44991)
                     /gene="mlaB"
                     /locus_tag="OO568_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6639922.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(44991..45626)
                     /gene="mlaC"
                     /locus_tag="OO568_000432"
     CDS             complement(44991..45626)
                     /gene="mlaC"
                     /locus_tag="OO568_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6639923.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45645..46196)
                     /gene="mlaD"
                     /locus_tag="OO568_000433"
     CDS             complement(45645..46196)
                     /gene="mlaD"
                     /locus_tag="OO568_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6639924.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46201..46983)
                     /gene="mlaE"
                     /locus_tag="OO568_000434"
     CDS             complement(46201..46983)
                     /gene="mlaE"
                     /locus_tag="OO568_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6639925.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHVPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(46991..47800)
                     /gene="mlaF"
                     /locus_tag="OO568_000435"
     CDS             complement(46991..47800)
                     /gene="mlaF"
                     /locus_tag="OO568_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6639926.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48010..48975
                     /locus_tag="OO568_000436"
     CDS             48010..48975
                     /locus_tag="OO568_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6639927.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLLLGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            48989..49975
                     /gene="kdsD"
                     /locus_tag="OO568_000437"
     CDS             48989..49975
                     /gene="kdsD"
                     /locus_tag="OO568_000437"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6639928.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            49996..50562
                     /gene="kdsC"
                     /locus_tag="OO568_000438"
     CDS             49996..50562
                     /gene="kdsC"
                     /locus_tag="OO568_000438"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6639929.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDCPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50559..51134
                     /gene="lptC"
                     /locus_tag="OO568_000439"
     CDS             50559..51134
                     /gene="lptC"
                     /locus_tag="OO568_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6639930.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51103..51660
                     /gene="lptA"
                     /locus_tag="OO568_000440"
     CDS             51103..51660
                     /gene="lptA"
                     /locus_tag="OO568_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6639931.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51667..52392
                     /gene="lptB"
                     /locus_tag="OO568_000441"
     CDS             51667..52392
                     /gene="lptB"
                     /locus_tag="OO568_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6639932.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52440..53873
                     /gene="rpoN"
                     /locus_tag="OO568_000442"
     CDS             52440..53873
                     /gene="rpoN"
                     /locus_tag="OO568_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6639933.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            53896..54183
                     /gene="hpf"
                     /locus_tag="OO568_000443"
     CDS             53896..54183
                     /gene="hpf"
                     /locus_tag="OO568_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6639934.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54301..54792
                     /gene="ptsN"
                     /locus_tag="OO568_000444"
     CDS             54301..54792
                     /gene="ptsN"
                     /locus_tag="OO568_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6639935.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54838..55692
                     /gene="rapZ"
                     /locus_tag="OO568_000445"
     CDS             54838..55692
                     /gene="rapZ"
                     /locus_tag="OO568_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6639936.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55689..55961
                     /gene="npr"
                     /locus_tag="OO568_000446"
     CDS             55689..55961
                     /gene="npr"
                     /locus_tag="OO568_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6639937.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56174..56806
                     /gene="yrbL"
                     /locus_tag="OO568_000447"
     CDS             56174..56806
                     /gene="yrbL"
                     /locus_tag="OO568_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6639938.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56803..57531)
                     /gene="mtgA"
                     /locus_tag="OO568_000448"
     CDS             complement(56803..57531)
                     /gene="mtgA"
                     /locus_tag="OO568_000448"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR6639939.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPDGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57528..58181)
                     /gene="elbB"
                     /locus_tag="OO568_000449"
     CDS             complement(57528..58181)
                     /gene="elbB"
                     /locus_tag="OO568_000449"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6639940.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58411..60747)
                     /gene="arcB"
                     /locus_tag="OO568_000450"
     CDS             complement(58411..60747)
                     /gene="arcB"
                     /locus_tag="OO568_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6639941.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSHRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60843..61772)
                     /locus_tag="OO568_000451"
     CDS             complement(60843..61772)
                     /locus_tag="OO568_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6639942.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62447..66907
                     /gene="gltB"
                     /locus_tag="OO568_000452"
     CDS             62447..66907
                     /gene="gltB"
                     /locus_tag="OO568_000452"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6639943.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIHPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            66920..68338
                     /gene="gltD"
                     /locus_tag="OO568_000453"
     CDS             66920..68338
                     /gene="gltD"
                     /locus_tag="OO568_000453"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6639944.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68522..68926
                     /locus_tag="OO568_000454"
                     /pseudo
     CDS             68522..68926
                     /locus_tag="OO568_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
BASE COUNT        16841 a        18751 c        17153 g        16253 t
ORIGIN      
        1 tgtcggtatt cgtattgaaa atctcggcgg cggagttgca aatattcctt ttcagaacgg
       61 tatccttccc gttgatcctt ccgggcatgg caccgtcaat atgcgcgcct ggccagttaa
      121 tctggtcggt ggtgagctgg aaacaggaaa atttcagggc acagccacca ttaccgtcat
      181 ggtgcggtaa aagaagacgt tgcgttttga ttaacgcaac gcttcggcac tgttacccct
      241 gctgctccag cgcatactta tacagcgcat ttttcttcac gccatgaatt tctgcggcca
      301 gcgccgccgc ttttttcagc ggcagttctg cctgtagcag cgccagcgtg cgcagggcat
      361 cggcgggtaa gtcttcttcc tgtgctttat gaccttcgac aatcagcacc atttcgcctt
      421 tgcgacggtt ttcatcttcc tttacccacg ccagcagctc gccaacgggc gcgccgtgaa
      481 tggtttccca ggttttggtc agctcacgcg ccagaaccac gtagcgggat tcgcctaata
      541 ccgcaacgat atcttccagg ctatctaaca gacggtgggt agattcataa aaaatcagcg
      601 tgcgcggctc cgcttcaatg gcttttagcg catcacggcg gccttttgat ttggcaggta
      661 aaaagccttc gtaacagaaa cggtcagatg gtaaacccgc ggcgcttaac gcagtgatag
      721 cagcacacgg tccgggtagc ggcaccacac gaatccccgc ttcacggcag gtacgcacca
      781 gatggtagcc aggatcgtta attagcggcg ttccggcatc ggaaaccagc gcaatgtttt
      841 gcccctcttg cagcttcgcc agcagcgttt cggctttttg ttgttcgtta tggtcgtgca
      901 gcgcaaacaa ccgggcatta atcccaaaat gttgcagcaa taaacctgtg tgacgagtat
      961 cctcggcggc aatcagatca acggcctgta atacctctaa cgtacgctgg gtgatatccg
     1021 ccagattgcc gattggcgtc ggtacaatgt aaagctggcc ctgagaatta tccgccgatt
     1081 ggtgttgttt cattgtgtcg tccgtattgc cgatttaata ttgagcattg cgtaaaaaaa
     1141 atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc gcgcgttgtc
     1201 tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact cccgatcagt
     1261 ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt cagcagatgc
     1321 agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt gcactggtga
     1381 aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa gaactgaacg
     1441 attctcagcg tcgcgagaaa acactgctgg cggcagagat taaactggcg cagaaagatt
     1501 ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa caaaaccagc
     1561 aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc attgatttac
     1621 tgcgcgcgtt aattgctcag gaaccgctgc ttggcgcgaa agaaaaacag cagaatattg
     1681 atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg ctggtgatca
     1741 acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg tttgataacc
     1801 gtaacgatcc cgacatgatg aaagccggga tcgccgactg gcagaaacgt tatccgaaca
     1861 atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt aaaccagcct
     1921 cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta tttggtcgca
     1981 ctattcagca aggctttgaa gccgcgaaaa atatcagcac tcagccagtg gcggctcagg
     2041 tagctgccgc acctgccgca gacgtagcag aacaacctca gccgcaaact gcggatggcg
     2101 ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgatcagcct gcggcccagc
     2161 cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc gcaaatccat
     2221 ccgcagagct gaaaatctac gatacctcat cacaaccact tagccagatc ttaagccagg
     2281 ttcagcagga tggcgcgagt attgtggttg gtccgttgct gaaaaataac gttgaagagt
     2341 tgctgaagag caacaccccg ctgagcgtac tggcactgaa ccagccggag aatatcgaaa
     2401 accgcgtcaa tatttgttac ttcgcgcttt caccggaaga cgaagcgcgc gatgcagcgc
     2461 gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc agtgcattgg
     2521 gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa actgggcggc ggcaccgttc
     2581 tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc ggttctggta
     2641 ttgctttaac gggtagcccg attactccca gagcgacaac cgactccggc atgacgacca
     2701 acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat ggcggtcgtg
     2761 tcgatgcggt ctacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa ccgatgatcg
     2821 ccatgcgtaa cggtagccag agcggtgcaa cgctgtacgc cagttcccgc agcgcacaag
     2881 gcaccgcagg cccggatttc cgactggaga tggaaggctt gcagtacagc gaaatcccga
     2941 tgctggcagg cggtaatctg ccgttaatgc agcaggcgct cagcgcggtg aacaacgatt
     3001 attcgctggc tcgcatgtat gcgatgggcg ttgatgcctg gtcgttggca aatcatttct
     3061 cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagt ctgacggcta
     3121 acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa ggtcaggtag
     3181 tccccgccag ttaaccacca aacagaccgg cgatgcgtgg gaagcacaag cgcgtcgctg
     3241 gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg aacgagcgtg gcggcgagat
     3301 cgatctgata atgcgtgaag gcctgaccac cgtttttgtc gaggtacgct atcgccgctc
     3361 tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca aattattaca
     3421 gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg attgccggtt
     3481 cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct ttaatgacca
     3541 ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc ttcactgaaa
     3601 gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc cgtgcagcca
     3661 tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt aatggaactt
     3721 ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa acggagcggc
     3781 ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg attgccaacg
     3841 atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat gcgggagatg
     3901 tattgttagc catttccacc cgtggcaaca gccgcgatat tgttaaagca gttgaagccg
     3961 ccgttacgcg tgatatgacc attgtggcat tgaccggcta tgacggcggc gaacttgcag
     4021 gtttgttagg gccacaggat gtggagatcc gcattccttc gcatcgtagt gctcgcattc
     4081 aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac acgcttttcc
     4141 ctcaccagga tgattaagga gaatacatga aggcattatc gccaatcgca gtccttattt
     4201 ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct gctgtgggta
     4261 ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt accctggaag
     4321 tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaaacc cgtattaatg
     4381 tcacggccta tcagggcaaa gtgctgctgg ttgggcagtc accaaatgct gaactttcgg
     4441 ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat aacgagattc
     4501 gtcagggcca gccgattggt ctgggcgaag catctaacga tacgtggatc accaccaaag
     4561 tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa gtgaccaccg
     4621 aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa gcggcggcag
     4681 atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg tttattaaat
     4741 agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc tacaacgatg
     4801 caaaccgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc tcaaatcaga
     4861 acaacagcgc cagcccgccg acaatcacac cagacactgc caccatcgcc cctgtcagcc
     4921 ataaggcttt cgccgggaac gctttgcgca gcataatcag tgacggcaaa ctcactgccg
     4981 ggagcgtcat caacaatgcc agcgccggag cggttcccat acctgccagc atcatcgttt
     5041 gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct accgccatcg
     5101 ccaccaccca catcaggccg ttatcgacag caccatcggc atgggggaat aaccagacgc
     5161 gtgcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc cagaaaagtg
     5221 tccatagcgc cctgccccag cggctaaaaa aaccgctctg tgcttccggt atgtcaattt
     5281 cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc gtcgcaatca
     5341 gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag ccgaggacaa
     5401 accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag aatgccagcg
     5461 caccgcccat cgatacctgt tggcgacgca ttcccgccgc gaccggagcc gcacagcagg
     5521 tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga aagcgcgatt
     5581 gcccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac gaaccgagga
     5641 tcacccccag caccgccgct ttccatactg cgaggaaata gatcatcgcg taatccaacg
     5701 ccgcttgcca cggattagca tccgcctgcg caaggataga tttaccgata ctgtgggttt
     5761 cggctgcagt aaacgctttg ccgtagtaag gttgccattt cacataccag agaccggcaa
     5821 tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc gcctgagatg
     5881 aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc gcgtgaatat
     5941 tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat tctgaagagc
     6001 ttaaaatcgt cacgccttca tgctcaggcc gcatcgcctc tgccagcatg acccgcgcca
     6061 cgtctctcgc atcaatggat ttccagttac ctggtaacaa acggaacagc ggcgcaaaaa
     6121 gcgtttcgtt catccgctgt ttgctacgat cgcccagtaa catcgacggg cgagcaatgg
     6181 tcaatttcgg ccagttctgg gcaattaatg cttcttccat ctcccctttg acgcggttat
     6241 agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata tgctgcgcgc
     6301 ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca tgaataaacg
     6361 cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca atgtcgatgg
     6421 gttcggtgac ctgcgccaac gcgtcactca gttgcggatc atggggatta aacaccccgg
     6481 gcatatcgcc caacggacgt cgcgtcggcg cagcaatggc gttaactttc ggttcattaa
     6541 tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc agtacctgac
     6601 tcatcttcgc tcctttacag aattgtctgc cttgcgctcc ccggctcatg caccagtctt
     6661 aatagaccat gaggtaatta tccccgattg tggaaaattc gcctcatcca atgcaacaac
     6721 gcggaggaag catgagtaag aaaattgccg ttttaatcac tgatgaattt gaagattcag
     6781 aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt actattgaaa
     6841 aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc atcgataaat
     6901 ccatcgatga agtgacgtca gcggagtttg atgccctgtt gctaccgggt ggtcattcac
     6961 cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt gtgaatagcg
     7021 gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc gatgtgattc
     7081 gggggcgcaa actgaccgca gttaaaccga tcattattga tgttaaaaat gcgggcgtgg
     7141 aattttacga tcaggaagtc gtggttgata aagatcagct ggttaccagc cggacaccgg
     7201 acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga gtcgcgtagc
     7261 caaatcattt ttttaccaaa gcccagcgtg ttgtcggtga atttgatttc atcgagccgg
     7321 atttcccaca ccggggccga cagcattctg gcaaccggaa agcgacgatt gtacgccttg
     7381 cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa ctgcacaccg
     7441 cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc agcctgcggc
     7501 ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc gactttctgc
     7561 gcatcaaaaa gataaaaggc attagcgcac cagagttccc cttcctgctg cacacaccag
     7621 gtgacaacat gttgttttgc cagccaacgg ctgatggcga tgagtgtttc cattgctgtt
     7681 ctcttttata ctgtgggcct gaccttaaca tgcgatgcag acgatgaccc cctggtttct
     7741 ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg atgtcgaacg
     7801 ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga aaggagaact
     7861 gacgctggcg ttttctgcgc cagtgggcga ccgttcgctg gcactacggg cggaatatcg
     7921 cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg cagggtttgc
     7981 agaactgttg agcagcctgc aaaccccgga gattaaaagc gattgaaatg ctcgtgatac
     8041 tcaaccagac cggttacgcc attcagcgcg tcatccgcta aacgatgtac ctggaaggcg
     8101 ctttcagtgc ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc aaagccgaaa
     8161 cgactgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa ctcattaagc
     8221 gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta tttttcatca
     8281 accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc aaccggacta
     8341 aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc cagcgtcaga
     8401 aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc gaatgagcga
     8461 cgcagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat tagcatggtt
     8521 cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca acaaaatccg
     8581 ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca atggcgtcca
     8641 tcaggttttt cacatacacc cccagctccg tatcgccttc aatcaccagc cgacgctgga
     8701 agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg ctggcgtcgg
     8761 cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca ttcaccaccg
     8821 aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg ccttcaagaa
     8881 actccagctc gccatcatcc agcgcctgac ggaattgcca gcttaagacc tgctcaagaa
     8941 cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga gatggcccca
     9001 aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt ttcaacaatc
     9061 atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat tccatatgaa
     9121 attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc gcagcaaggt
     9181 taactaaaat cccagttcgt taacattttt gcgttttgat agcgcaacct tcaggaaaaa
     9241 ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc atcgaaaacg
     9301 gcgcagatgc tgtttatatc gggctaaaag atgataccaa tgcccgtcac ttcgccggcc
     9361 ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa catcgccgca
     9421 aacttcacat ctcgattaac acttttgcgc atccggacgg ttacgcccgt tggcagcgtg
     9481 ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac ctcgccatgc
     9541 tggagtacgc cgccgagcgt tacccgcata ttgaacgtca tgtgtcagtg caggcttcgg
     9601 cgaccaatga agaggcgatt aacttttatc atcgccattt tgacgttgcc cgcgtggtgc
     9661 tgccgcgcgt gttgtcgatt catcaggtga aacagctggc acgggttact cctgtcccgc
     9721 tggaagtgtt tgctttcggc agcctgtgca ttatgtcgga aggccgttgc tatctgtcgt
     9781 cgtatctgac gggtgagtcg cccaacactg tgggtgcgtg ttctccggcc cgtttcgtgc
     9841 gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg atcgaccgtt
     9901 atcaggacgg cgaaaacgca ggttatccga cgctgtgtaa agggcgttat ctggtggacg
     9961 gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa ctgctgccgg
    10021 agttaatggc tgcgaatatt gcttcggtga aaattgaagg ccgccagcgt agcccggcgt
    10081 atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag gccgatccgc
    10141 aaaacttcgt accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc gaaggcacgc
    10201 aaaccaccct tggcgcgtat caccgtaaat ggcagtgaga agagcaatga aatattcctt
    10261 agggccagtg ctgtggtact ggccaaaaga gacgctggaa gatttttatc agcaagccgc
    10321 cgccagcagc gccgacgtga tttatcttgg tgaagcggta tgcagcaagc gccgggcaac
    10381 caaagttggc gactgtctgg agatggcaaa atcgctcgcc ggaagtggca agcagattgt
    10441 gctctccacg ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga aacgctatgt
    10501 tgagaacggt gagtttctga ttgaagccag cgatctcggc gtggtgaata tgtgcgctga
    10561 acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg tgacgctgaa
    10621 aatattgctc aaacagggca tgatgcgctg gtgtatgccg gtggagcttt cccgcgactg
    10681 gctggtgaat ctgcttaatc agtgcgatga gctgggcatt cgcaaccagt ttgaagtgga
    10741 agtcctgagc tacggtcatc taccgctggc ctactccgcc cgctgcttta ccgcgcgttc
    10801 ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga acgggcgcaa
    10861 cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat ggcattcaga ccatgagcgg
    10921 ctacgtttac aacctcggta acgagctggc atccatgcag ggattggttg atgtagtccg
    10981 cctgtcaccg cagggcactg acactttcgc gatgctcgac gccttccgcg ctaatgaaaa
    11041 tggcgctgtg ccactgccgt tgacggcaaa cagcgactgt aacggctact ggcggcggct
    11101 ggcaggactg gagctgcaag cctaagtaaa tagctcactt tgttaacaac tttaactatt
    11161 ctttaatgca gtattaaaga ttaatcggta acaaagtgag ctgttatgac tgataaaacc
    11221 attgcgtttt cgctactcga tctggccccc atacccgaag gctcttcagc gcgggaagcg
    11281 ttctcccact ctctcgatct cgcccgtctg gctgaaaagc gcggctatca tcgctactgg
    11341 ctggcagaac accacaatat gaccggcatt gccagtgctg caacatcggt attgattggc
    11401 tatctggcgg cgaataccac cacgctgcat ctggggtctg gcggcgtgat gttgcctaac
    11461 cactcaccgt tggtcattgc cgaacagttc ggcacactca atacgctcta tccggggcga
    11521 atcgatttgg ggctgggtcg tgctccgggt agtgaccaac ggacaatgat ggcgctacgt
    11581 cgtcatatga gcggcgatat tgataatttc ccccgcgatg tcgcggagct ggtggactgg
    11641 tttgacgccc gcgatcccaa tccgcatgtg cgcccggtac caggctatgg cgagaaaatc
    11701 cccgtgtggt tgttaggctc cagcctttac agtgcacaac tggcggcgca gcttggtctg
    11761 ccatttgcgt ttgcctcaca cttcgcgccg gatatgttgt tccaggcgct gcatctttat
    11821 cgcagtaact tcaaaccgtc ggctcggctg gaaaaaccat acgcgatggt gtgcatcaat
    11881 attatcgccg ccgacagcaa ccgcgacgct gaatttctgt ttacctcaat acagcaagcc
    11941 tttgtgaagc tgcgccgtgg cgaaatcggg caactgccgc cgccgattca aaatatggat
    12001 cagttctggt caccgtctga gcagtatgac gtgcagcagg cgctgagtat gtcgttggta
    12061 ggcgataaag cgaaagtgcg tcatggtttg cagtcgatcc tgcgcgaaac cgacgctgat
    12121 gagattatgg ttaacgggca gattttcgac caccaggcgc gcctgcattc gtttgagctg
    12181 gcgatggatg ttaaggaaga gttgttggga tagtgtgtct taacgcggga agccttatcc
    12241 gagctggcaa cgctgtccta cgtaggcctg ataagcgaag cgcatcaggc attgtgtagg
    12301 cagcagaaat gtcggataag gcaccgctga ttactgatac accggcagta aattaaagct
    12361 cgataaaata tgcaccagtg cgttgccgac gccaaacacc agaatcagcg caatcatcgg
    12421 cttgccaccc cagacgcgga atttcgggct gccaaagcgt ttacgcgatg cgcgggccag
    12481 cagtgccgga acaattgccg cccagatggt agccgctaaa ccagcataac caatggcata
    12541 caggaatccg ttcgggaaca gcagaccacc cacaacaggc ggggcaaagg tcagcaatgc
    12601 cgttttcaag cggcccacag ccgagtcgtc gaaaccaaac agatctgcca gatagtcaaa
    12661 caaacccagc gttacgccaa ggaacgaact cgctaccgca aagtttgaga acacgaccag
    12721 cagaaggtcc aggctacggc tgttcagcac gccgcttaat gcttgtacca gcacatcaat
    12781 attaccgccc ttctcggcga tgccgataaa ctccggacga gggatgttgc ccattgtcgc
    12841 cagcaaccag atggtataca gcgccagtgc catcagcgta ccgtacacca ggcatttcac
    12901 gatggttttc ggatctttgc cgtaatactt catcaggctt ggcacgttac cgtgataacc
    12961 aaacgacgcc aagcagaacg gcagggtcat cagcaaatac ggcgcataag atgcattgct
    13021 ttcggcaacg ttgaacaatg tcgcaggctg cacatgcccc agcaggctac cgaaggtgag
    13081 gaagaaggta atgactttcg cccccagcac aatcgccgtc atgcggctga cggctttagt
    13141 actcaaccac accacaaacg ctaccagcag tgcaaaacca aaacccgccg cccgtgccgg
    13201 gacgtttagc gacatctctg cgaaggtgtg atgcagaatc gaaccactgg cagaaatata
    13261 ggcataggtc aggatataga gcacaaaggc aatggaaatg ccgttgacca cgttccagcc
    13321 tttgcccagc aaatctttgg tgatggtgtc aaaactcgaa ccgattcggt aattcaggtt
    13381 agcttccaga atcatcaagc cggaatgcag catacagaac caggtaaaga tcagcgctgc
    13441 cattgaccag aaaaaccacg ccccggacat gaccactggc agagaaaaca tccctgcgcc
    13501 aataatggtg ccgccgataa tcaccacgcc gccaagcagc gacggtgacg tttgggtggt
    13561 ggttagtgtt gccatgaggg cttctctcca gtgaaaaata gtgcgactgc gttgttatgc
    13621 attgcactgt accagtacat gagtacaaaa gacagaaaaa aagccccgat ggttaaaatc
    13681 ggggctgtat atattatttt acagattgtg ttcgctgttc agcgatgatt acgcatcacc
    13741 accgaaacga cgacgaccgg tagagtcatc acgacgcggt gcgcggcctt cacgacgttc
    13801 gccgctaaaa cgacgaccat caccacgacc accttcacgg cgttcaccgc tgaagttacg
    13861 accgccttca cgacgctcgc caccgaaacc acgaccaccg ccacgaagct caccgccagt
    13921 atgcggctgt gcatcgccca gtaactgcat gttcatcggc ttgttgagaa tgcgagtgcg
    13981 cgtaaagtgt tgcagcactt cacccggcat acctttcggc agttcgatgg tggagtgaga
    14041 agcaaacagc ttgatgttac cgatgtaacg gctgctgatg tcgccttcgt tagcaatcgc
    14101 gccaacgata tgacgaactt caacaccatc atcgcggccc acttcaatgc ggtacagctg
    14161 catatcgcca acatcacgac gttcacgacg cggacgatct tcacggtcgc cacgcgggcc
    14221 acggtcgtta cgatcgcgcg gaccacggtc atcacggtca cggaattcac gtttcggacg
    14281 catcggcgca tctggcggaa caatcagagt acgttcaccc tgtgccattt tcagcagtgc
    14341 cgcagccaga gtttcgagat ccagctcttc accttctgca gtcggctgaa ttttgctcag
    14401 cagtgcgcgg tattgatcca gatcgctgct ttccagctgc tgctgtactt tagcggcgaa
    14461 tttttccaga cggcgtttgc ccagcagttc tgcgttcggc agttctactt ccggaatagt
    14521 cagcttcata gtacgttcaa tgttgcgcag cagacgacgc tcgcggttct caacgaacag
    14581 cagcgcgcgg ccagcacgac ccgcacgacc ggtacgaccg atacggtgaa cgtaagactc
    14641 agaatccatc gggatatcgt agttaaccac caggctgata cgctcaacgt ccaggccacg
    14701 ggccgcaacg tcggtcgcaa tcaggatgtc cagacgaccg tctttaaggc gttccagtgt
    14761 ctgctcacgc agcgcctggt tcatgtcacc gttcagcgcg gcgctattgt agccgttacg
    14821 ctcaagagct tcagccactt ccagagtcgc gtttttggta cgaacgaaga taatcgccgc
    14881 atcaaaatct tccgcttcca ggaaacgtac cagtgcttcg tttttacgca taccccacac
    14941 agtccagtag ctctggctga tgtcaggacg ggtagtcacg ctggactgaa tacgcacttc
    15001 ctgcggctct ttcataaagc ggcgggtaat gcgacgaatc gcttccggca tggttgcaga
    15061 gaacagagcg gtctgatgac cttccgggat ctgcgccata atggtttcaa cgtcttcgat
    15121 gaagcccatg cgcagcattt cgtcagcttc atccagaacc agaccgctca gtttagagag
    15181 gtccagagtg ccacgtttca ggtggtccag cagacggccc ggagtaccga caacgatctg
    15241 cggcccctga cgcagggcgc gtaattgcac gtcataacgc tggccgccgt acagggcaac
    15301 cacatttacg ccgcgcatgt gtttagagaa atccgtcatt gcttcagcaa cctgtaccgc
    15361 cagttcgcgg gtcggtgcca gcaccagaat ctgtggtgct ttcagctcag gatcaagatt
    15421 ctgcagcaga ggtaaagaga atgctgcagt ttttccgctc cccgtctggg ccatacccag
    15481 aacgtcgcgg ccattcagca gatgtggaat acactctgcc tgaattggag atggtttttc
    15541 gtaacccaga tcgttaaggg cttcaaggat aggagccttc aggcccagat ctgcaaaagt
    15601 ggtttcgaat tcagccatgt agtacgtgtg cctcaaaatt aatggcggcc agtctacata
    15661 actcatcatg aaattgatca gcaattttca ttgaaaagtg tgaaccggct caaagtaggt
    15721 gtattaacga acaacaacgc cctcacccgt taaggtgatg gcaatcaaaa aagattacgg
    15781 gctgatgtgt acgtcagcta ttgctggtcc gattctgcca ggtcatcttg gtcctggccc
    15841 aggagcgata attccaacaa tgcgtatcgg tgctcaacaa agttatgaac gttgttggca
    15901 accgccagtt tgaacagtgc cgtggcgctg tccaaatccc ccagacttag gtagtactta
    15961 cctaaataga agttggtttc actgagatgc tcagcgagcg aggtgttatc ggttgcgtcc
    16021 gccttgagcc tttccattaa cgtttgttcg ctaatgttgc ccaggtagaa ctcgacaatg
    16081 ttccatcccc actgttcctt atccgatttt tcgaagtgct gtttcaacac ttctttagcc
    16141 tgcttctcat cgagcttctg ctcggcgaga taaagccaca gactacggaa aggatcattg
    16201 ggatcgtctt gataaaacgc cagcagatca tcttgcgcta acttgtcacg accgccgtaa
    16261 tataatgcaa tcccgcgatt caagtgcgcg tagttgtaag ttggatcaag ctcaagtaca
    16321 gaatcaaacg cttcataggc agcatcaaaa ttgcctgcct gcgttaaata tatgcctaag
    16381 taattgaata cttcaggcat atccggtcgg attgccagcg cttgcgaaaa atcgttacgc
    16441 gctaatgccc tcagaccgag actatcatac aacactccgc gctcatataa aagctgtgcg
    16501 cgttcgtcat cggttaaagc ccgactggca aggatttgtt ccatacgtgc cagaatcact
    16561 tcctgctgta aagtcggttg caatggtacc gcgaggactt cacttttacg ccaggaagta
    16621 ttactgcatc ctgcaagcgt aagtgctgtc gcaacgaaac accagcgcaa aaaaggcttc
    16681 atttcccact cccgaagacc acggttgaat gaacgtcctg ttcccggttg ctaacaaggc
    16741 gtcctgcccg gttaaaagcc ccccgccgca gcggagggca aatggcaacc ttactcgccc
    16801 tgttcagcag ccggagcttc cggtgctgca gcaggttgag actgctcagt cgcttcttta
    16861 atgctcagac ggatacggcc ctggcgatca acttccagaa ctttcaccgg tacttcctga
    16921 cccatctgca ggtaatcggt cactttctca acgcgtttgt cagcgatttg agagatgtgt
    16981 accagacctt ctttaccgcc gccgatggca acaaatgcgc caaagtcaac gatacgggtc
    17041 actttaccag tgtagacgcg gcccacttcg atttctgcag tgatctcttc gatacgacga
    17101 atagcatgtt tcgctttctc gccgtcggtc gctgcaatct tcacagtacc gtcatcttcg
    17161 atttcgatgg tggtgccagt ttcttcggtc agggcacgga taacagagcc gcctttaccg
    17221 ataacgtctt tgatcttgtc cgggttgatc ttgatggtat ggatacgcgg tgcgaactca
    17281 gagatatcgc cacgcggcgc gttgatcgcc tgttccatta cgcccaggat atgcagacgc
    17341 gcacctttag cctggttcag cgcaacctgc atgatctctt tggtgatacc ttcaattttg
    17401 atatccatct gcaatgcaga gataccctcg cgggaacccg caactttgaa gtccatatcg
    17461 cccaggtgat cttcgtcgcc caaaatgtca gacagtacaa cgtagttgtc gccttctttc
    17521 accagaccca ttgcgatacc cgcaacggca gctttgatcg gcacacctgc gtccatcagc
    17581 gccagagacg cgccgcacac ggaagccata gaagaggaac cgttggattc agtgatttca
    17641 gacacaacac gtacggtgta cgggaatttg tccatatccg gcatgactgc cagcacgccg
    17701 cgcttcgcca gacgaccgtg accaatttca cgacgcttcg gagaaccgac catgccggtt
    17761 tcgcctacgg agtacggagg gaagttgtag tggaacagga aggtatcggt acgttcgccc
    17821 atcagttcat caagaacctg cgcgtcacga gcagtaccca gcgttgcggt aaccagcgcc
    17881 tgcgtttcac cacgggtgaa cagcgcagaa ccgtgagtac gcggcagcac gccagtacgc
    17941 acatccagac cacggatcat atctttttca cgaccgtcga tacgcggttc gcctgccagt
    18001 acgcggctac gaacaacgtt tttctcgatc gcgtgcagaa tttcacccag ttcgttttcg
    18061 tccagggttt cgtcttcagc aagcagcgtc gcgatggttt cagatttgat gacatcaacc
    18121 tgcgcataac gctcttgttt gtcggtgatg cggtaagcat cgctcaggcg agcttcagcc
    18181 agtgcagcaa cgcgcgcgtt cagcgcttcg tttaccggct ccggctgcca gtcccaacgt
    18241 ggtttaccgg cttctttcac cagttcattg atgttctgaa taacaacctg ctgttgttca
    18301 tgaccgaaca ctactgcgcc cagcatctgg tcttcgctca gcagttcagc ttcagattca
    18361 accatcagta cagcggcttc agtaccggca acaaccagat ccagtttgct ctctttcaac
    18421 tcgtcctgag tcgggttcag tacgtactgg tcattgatgt aacctacgcg agcagcacca
    18481 atcggaccat tgaacggaat accagacaga gacagtgctg cggaagcacc aatcatcgcg
    18541 acgatatccg ggttaacttg cgggttaaca gaaaccacgg tggcgataac ctgaacttcg
    18601 ttgacgaagc cttccgggaa cagcgggcga atcgggcggt caatcagacg cgcgatcagg
    18661 gtttcgcctt cgcttgggcg gccttcacga cggaagaagc tacccgggat acgaccagca
    18721 gcgtaggtac gctcctgata gttaacggtc agtgggaaga agtcctgacc tggtttggct
    18781 tttttctggc caacaacggt aacaaatacc gaggtgtcat ccatgctaac cataacagcg
    18841 gcagtagcct gacgagccat catgccggtt tccagagtca cggtgtgttg gccgtactgg
    18901 aatttacgaa cgatcggatt aagcaatgta atatcctttc tctttcttag acagtacctt
    18961 acggcactgg tgttaatacc cgatcttctg cgcatcctcg cgactaatga caaccctaac
    19021 ccagctctat gtgggtaaag cctctcatta gccgcgcgaa cctctgcaac ggaagatcat
    19081 tcatagcaac aatacattag tttccagtga attgctgccg tcagcttgaa aaaaggggcc
    19141 actcaggccc ccttttctga aactcgcaag aattagcgac gcagacccag gcgctcgatg
    19201 agctgggtgt aacgtgctac gtctttacgt ttcaggtagt cgagcagttt acgacgctga
    19261 gaaaccatgc gcagcagacc acgacggctg tggtgatctt ttttgtgctc tgcaaagtgg
    19321 ccctgcaggt ggttgatctg tgcagtcagc agtgctacct gaacttcagt agaaccggtg
    19381 tcgtttgcgt cacgaccaaa ctcagaaacg attttagctg ttgcttcagt acttagagac
    19441 attttaaaac tccaaagtat atagaatgaa aggacgccga tctctaattc agcgatccca
    19501 gtgtacgtta cgcaaagtgt taaataattt acgcgacgtt aagcggcagt attctactcg
    19561 tagcgacctg ttatcgcaag acggttaaca ttacgccggg tattcaacca ccaggcgacg
    19621 aggcgcaacg cggccttcat cgtcaatttc gcccataccg ataaatttgc cgttctcacc
    19681 ttctgtaacg cgaaccagtc cttccagcgg cgcaccggat gtacgaactg ggttaccatt
    19741 tttgaagtaa acagaagacg ttaacggaag attcaccacc gggtagtccg aagctggact
    19801 gtccattggc atcagtaatg gatcaagtaa ctccgcagcc ggaatatcct gctgttcggc
    19861 ttgttcgacc agttcacgca gatgttccag ggtcacaatc cgttcaaccg gatatttact
    19921 taccgccaga cgacgcaggt aaataacatg cgcgccacaa ccgagttttt cacccaggtc
    19981 atcaatgatg gtgcggatat aagtgccttt tgagcagtga atttccagct ccagctcatt
    20041 gccttcgtgg cgaataaaca gcaattcata aacggtaatc gggcgcgctt cacgcggtac
    20101 ttcaatgccc tgacgcgcat attcgtacag ttttttaccc tgatatttca gtgctgaata
    20161 catcgaaggg atctgttcga tatcgccacg gaaagtatcc agcgccgccg ccagttgctc
    20221 tgcgctaaag gttaccggac gttcttcaac gatttgtccg tcggcatcag aagtatcggt
    20281 acgctgtcca agacgcgcaa tgacccgata gcgtttgtcg gagtccagca gatactggga
    20341 aaacttcgtc gcttccccga ggcaaattgg caacatgcca gttgccagcg ggtccagcgc
    20401 accggtatgc ccggcacggt tggcgttata aatccgtttc actttttgca gcgcatcatt
    20461 gctggacata ccctgcggtt tatccagcaa caaaacgccg ttaatgtcgc gaccgcgacg
    20521 acgaggacga ctcattagtc ctccttgctg tcgtccgggt taacacgacg ttcttcgtca
    20581 tgtttgacca cgctggtcac caggtttgac atgcgcatcc cttcaaccag agagttgtcg
    20641 tagaagaagg tcagttccgg cacgatacgc aggcgcatcg ctttccccag caggctgcgg
    20701 atgaaaccag aagcttcttg caacgctttg atgcccgctt taaccgcgtc ttcatctttg
    20761 tcgttgagga acgtcacata tactttggca tacgccaggt cgcgagacat ttcgacaccg
    20821 gaaacggtgg tcatcatgcc caggcgagga tctttaattt cacgctgcag gatgagagcg
    20881 atctcttttt gcatttcctg cgctacgcgc tgcgggcgac caaattcttt cgccataata
    20941 aattctcctg acaaaaaagg ggctgttagc ccctttttaa aattaatttc aggtggaagg
    21001 gctgttcacg ttggcctgat aagacgcgcc agcgtcacat caggcaatcc atgccggatg
    21061 cggcgtaaac gccttatccc gcatggaacc ctaaaaacct taagcgatgg tacgttggat
    21121 ctcgatgatt tcgaatactt cgatcacatc gccagtgcgg acgtcgttgt agttcttaac
    21181 gccgatacca cattccatac cgttacggac ttcgttaacg tcatctttga agcggcgcag
    21241 ggactccagc tcgccttcgt agataaccac gttgtcacgc agaacgcgga tcgggttgtg
    21301 acgtttaacc acaccttcgg taaccataca gcctgcgatg gcaccaaatt tcggcgattt
    21361 gaacacgtca cgaacttccg ccagaccgat aatctgctgt ttcagttccg gagacagcat
    21421 accgctcatc gccgctttca cttcgtcaat caggttatag atgacggagt agtaacgcag
    21481 atccaggctt tccgcttcaa tcactttacg tgcagaggca tcagcacgta cgttgaagcc
    21541 aaccaggatg gcgttggacg ccgcagccag ggtggcgtcg gtttcggtga taccacctac
    21601 gccagaaccg atgatcttca ctttaacttc gtcagtagac agtttcagca aggagtcgga
    21661 gatcgcttcc acagaaccct gtacgtctgc cttcaggacg atgttcactt cgtgaacttc
    21721 gccttcggtc atgttggcga acatgttctc gagtttagat ttctgctgac gcgccagttt
    21781 aacttcgcgg aatttaccct gacgatacag tgcaacttca cgcgctttct tctcgtcacg
    21841 tacaacggta acttcatcac ccgcagccgg tacgccggac aggccgagga tttccaccgg
    21901 aatggacgga cccgcttcca gcacttcctg acccagttcg ttacgcatcg cacgaacacg
    21961 accgtattcg aagccacaca gaacgatatc gcccttgtgc agagtacctt cacgtaccag
    22021 aacggtagca accggaccac gacctttatc gaggaaggat tcgataaccg caccgctcgc
    22081 cataccttta cgtaccgctt tcagctccag aacttccgcc tgcagcagga tagcgtccag
    22141 cagttcatcg ataccagtac ccgctttcgc agacacatgt acgaactggc tttcaccgcc
    22201 ccactcttcc ggcaggatgc cgtactggga gagttcgttc ttaacgcgat ccggatcggc
    22261 ttctggttta tcgatcttgt tcactgcaac gaccaccgga acctgcgccg ctttcgcgtg
    22321 ctggattgct tcgatcgtct gcggcattac gccgtcgtcg gcagcaacaa ccaggactac
    22381 gatgtccgtt gcctgcgcac cacgagcacg cattgaagta aacgcggcgt gccccggggt
    22441 gtccaggaag gtgatcatgc cgttttcagt ttcaacgtgg tatgcaccaa tgtgctgagt
    22501 aatgccgccc gcttcgccag aggccacttt cgttgaacga atgtagtcca gcagagaggt
    22561 tttaccgtgg tcaacgtgac ccatgatggt cacaaccggc gcgcgcggtt cagccgcagc
    22621 acccgtgtca cggtcgctca ttaccgcctc ttccagctcg ttttcacgac gcaggataac
    22681 tttatggccc atctcttcag caaccagctg tgcggtttcc tgatcgataa cctggttgat
    22741 ggttgccatt gcgcccagtt tcatcatcgc tttgatgacc tgagagcctt taaccgccat
    22801 cttgttcgcc agttcgccaa cggtgatagt ttcgccgatc acaacgtcac ggttaacggc
    22861 ctgagcaggc ttctggaagc cttgctgcag cgaagaacct ttacgttttc cgcctttacc
    22921 gccacgtact gctgcgcgtg cttcttcacg atcagctttt gattcagcgt gtttgttgcc
    22981 tttcttcgga cgcgctgctt tcgcgttacg accacggcca cggccgcctt cgacttcacg
    23041 atcgctttcg tcttctgcct ggcgagcatg ttgagaagta gtgacgtgat aatcgctgga
    23101 atcttcagtc ggttccgcgt tatcagtcca tttgttttct tccgccatac gacgtgcttc
    23161 ttcagcaacg cgacgtgctt cttcttcgag tttacgacgc gcttcttctt cagctttacg
    23221 cttgagctct gcagcttcct gctcacggcg ggctttttca gcctgggcgt ttttagtcat
    23281 atcgtcttgt tgattgctca ctttgtcttt ttccgcagct tcacgtttcg cttgttcagc
    23341 agcttcacgc ttagcttgtt ctgcggcctc acgttcagct ttttgttgcg cctcgcgttt
    23401 agccgattct tctgcctcac gacgggcttg ctcttccgct gcaaggcgtt cagcctcttg
    23461 cggatcgcgt ttcacaaagg tgcgtttctt gcggacttcg atttgtaccg atttgctttt
    23521 tccaccggta ccaggaatgt taagggtgct gcgtgtttta cgttgcagcg tcaatttgtc
    23581 cgggcctgaa tttttctgat tcaggtggtc aatcaaagtc tgtttctctt gtgcagacac
    23641 agagtcgtca gcagacttcc ggatacctgc atcagcaaat tgctgtacca ggcgttccac
    23701 ggaggtctgt cgctctgcgg ccagcgtttt aatcgttaca tctgtcatgc tgttccttcc
    23761 tgctacagtt tattacgctt cgtcaccgaa ccagcaaata ttacgggcag ccataatcag
    23821 tgctccggct ttttcgtcgg tcaacccttc gatatcagcc agatcatcaa tgccctgttc
    23881 ggcgagatct tccagcgtac aaacgccacg ggcggccagt ttgaatgcca aatcacgatc
    23941 taccccttca aggttcagca gatcgtcagc cggtttgtta tcaccgaggc tttcttcctg
    24001 ggcctgtgca atggtggcca gtgcattttt agcacgctcg cgcagtgctt caacggtcgg
    24061 ctcatcaagg ccttcgattt ccaacagctc tttcatcggc acataggcca gttcttccag
    24121 cgtcgagaaa ccttcttcta ccagaacagt cgcgaagtct tcgtcgatgt cgagatattt
    24181 ggtgaaggtg tcgatcgctg cgtgcgcttc cgcctgatgc ttagcctgca ggtcgtcaac
    24241 ggtcattacg ttgagttccc aaccgctcag ctgcgaagcc agacgcacgt tctgaccgtt
    24301 acggccaatc gcctgcgcca ggttaccggc ttcaacggcg atatccatgg tgtgtttatc
    24361 ttcatccacc acgatagaag caacgtctgc cggtgccatt gcgttaatca caaactgcgc
    24421 cgggttatca tcccacagga cgatatcgat acgctcgccg cccagttcag tagaaaccgc
    24481 ctgaacacgc gcgccacgca tacctacgca agcacctacc ggatcgatac gtttatcgtt
    24541 ggttttcacc gcgattttcg cacgagaacc cgggtcgcga gccgctgctt taatttcaat
    24601 cacttcttcg ccgatttccg gcacttcaat acggaacagt tcgatcagca tttccggctt
    24661 ggaacgagtg acgaacagtt gcgcgccacg cgcttccggg cgaacggaat aaagcacgcc
    24721 acgaacgcgg tcgccagggc ggaagttttc acgcggcagc atatcttcgc gcaggatcac
    24781 ggcttcagca ttgttgccca gatccagaga gatgttgtcg cggtttactt ttttcaccac
    24841 gccggtgatg atttcacctt cgtgttcacg gaactgatca accaccatcg cacgttcggc
    24901 ttcacgcact ttctgcacga taacctgttt tgccgtctgg gtggtgatac ggtcaaaggt
    24961 aacagactca atctgatctt caacgtaatc gcccaagttc aggctttcat cttcataacg
    25021 tgcggcttca agggtgattt ccttggtcgg ctgggtgact tcatcaacaa ctaaccagcg
    25081 acggaaggtg tcaaaatcac cgcttttgcg atcgatctgt acacggacgt cgatctcttg
    25141 ctcatatttt ttctttgttg ctgtcgccag cgcgctttcc aatgcttcga aaatcttctc
    25201 gcgaggtagc gccttttcat tggatacggc ttcaactaca gccaaaattt ctttgttcat
    25261 cgcgggcttt tcacctcatc cagactatta aaagtgggga accaggttcg ccttctggat
    25321 attactcagc gcgaacactt catctttacc ttcgacggta actgtgatca tttcaccgtc
    25381 taccgctttg ataacgccct gccatttacg acggttttgt accgccatac ggagaaccag
    25441 agtcacctct tctccgacaa aacgggcgta gtgttcagcc gtgaacagtg ggcgatcgag
    25501 acccggtgag gagacttcca ggttataagc aacggtgatg ggatcttcaa catccagcac
    25561 agcacttacc tggtggctca catcagcaca atcatcaaca ttgatgccat cttcactatc
    25621 aatatagatg cgcagtgtgg atgtgcgacc gcgaataaat tcgatgccta ccagttcaaa
    25681 acccagggcc tcaactggcg cagtaatcat ctctgttaat ttttgctcta atgtggacaa
    25741 gcccaccccc aagacataaa aaaagggcct aaagcccagt tattctgtag tcagataaca
    25801 aaaaaccccg ataaatcggg gctttatata actgaaccct ataaccgcaa ctgcggtctg
    25861 gagcactttc cagaaggatt ttttcaaatc ccactacgaa ggccgaagtc ttcacagtat
    25921 atttgaaaaa ggactctaag ggaaagtggt tgcgggggcc ggatttgaac cgacgacctt
    25981 cgggttatga gcccgacgag ctaccaggct gctccacccc gcgcctgaaa cgtggcaaat
    26041 tctactcgtt ttgggtaaaa aatgcaaata ctgctgggat ttggtgtacc gagacgggac
    26101 gtaaaatctg caggcattat agtgatccat gccacatttt gtcaacgttt attgctaatc
    26161 acgtgaatga atatccagtt cactttcatt tgttgaatac ttttgccttc ccctgctctc
    26221 ccttaagcgc attattttac aaaaaacaca ctaaactctt cctgtctccg ataaaagatg
    26281 attaaatgaa aactcattta ttttgcataa aaattcagtg agagcggaaa tccaggctca
    26341 tcatcagtta attaagcagg gtgttatttt atgacgacga ttctcaagca tctcccggta
    26401 ggtcaacgta ttggtatcgc tttttccggc ggtctggaca ccagtgccgc actgctgtgg
    26461 atgcgacaaa agggagcggt tccttatgca tatactgcaa acctaggcca gccagacgaa
    26521 gaggattatg atgcgatccc tcgtcgtgcc atggaatacg gcgcggagaa cgcacgtctg
    26581 atcgactgcc gcaaacaact ggtggccgaa ggtattgccg ctattcagtg tggcgcattt
    26641 cataacacca ccggcggcct gacctatttc aacacgacgc cgctgggccg cgccgtgact
    26701 ggtaccatgc tggttgctgc gatgaaagaa gatggcgtga atatctgggg tgacggtagc
    26761 acctataaag gaaacgatat cgaacgtttc tatcgttatg gtctgctgac caatgctgaa
    26821 ctgcagattt acaaaccgtg gcttgatact gactttattg atgaactggg cggccgtcat
    26881 gagatgtctg aatttatgat tgcctgcggt ttcgactaca aaatgtctgt cgaaaaagcc
    26941 tactccacag actccaacat gcttggtgca acgcatgaag cgaaggatct ggaatacctc
    27001 aactccagcg tcaaaatcgt caacccgatt atgggcgtga aattctggga tgagagcgtg
    27061 aagatcccgg cagaagaagt cacagtacgc tttgagcaag gtcatccggt tgcgctgaac
    27121 ggtaaaacct ttagcgacga cgtagaaatg atgctggaag ctaaccgcat cggcggtcgt
    27181 cacggcctgg gcatgagcga ccagattgag aaccgtatca tcgaagcgaa aagccgtggt
    27241 atttacgaag ctccggggat ggcactgctg cacattgcgt atgaacgtct gttgaccggt
    27301 attcacaacg aagacaccat tgagcagtat cacgcgcatg gtcgtcagtt gggccgtctg
    27361 ctgtaccagg ggcgttggtt tgactcccag gcgctgatgc tgcgtgactc tctgcaacgc
    27421 tgggttgcca gccagatcac tggtgaagtt accctggagc tgcgccgtgg gaacgattat
    27481 tcaatcctga ataccgtctc agagaacctg acctacaagc cagagcgtct gacgatggaa
    27541 aaaggcgact cggtgttctc gccagatgat cgtattggtc aattgaccat gcgtaatctg
    27601 gatatcactg atacccgaga gaaacttttt ggttatgcca aaactggcct gctttcctcc
    27661 tctgccactt caggcctgcc gcaggtggag aatatggaaa acaaaggcca gtaattcgac
    27721 atcaaccctg cccttcaggg gtggggttac cttgcttatc ctctgtaaac tcctttctga
    27781 gcatcacata atcgaaaaca tcatggtttg catccacaac ccgtgagtct cctttatagt
    27841 gtgcaatcac ttcctcaagc gtttgcggct gttccggctt tgttaccccc atccacgcat
    27901 taataaggta attattgtaa gctgtcgaaa agatattgtt ttccgttgtg cgatccacgc
    27961 catccccaag aacggggcta taccagataa acatcgggac atgatatgcc tgctggctag
    28021 cctccctgcc tccatgaaaa tagacgttct tcttcgtagg gtcacgttcc aggccgtggt
    28081 cggcaaaata catgaccgag gcgcggcgat cttttaataa ttcaaaaacc tgacctagca
    28141 aactatctgt gtaatgaatg gagttgtcat agcaggcatc ctgatcgtcc tgcggttgaa
    28201 acacggcgct ggattgcgga taggcgctac aagccggttc atggcttcca tttaaatgaa
    28261 gaacaatcag tttcttttgc tgcgtatttt gctgtaatgc ttgcgataaa tgcggcaaca
    28321 acaattcatc aaatcctctg acataaactg tttccatggc gcgcatggcg atactggtaa
    28381 ctgctgtacc attctgccga aaagcggatt gcgagcttag ccagaaagtc tgaaatcctg
    28441 cttgattagc catattaata atgttgtcgg ggtaattatg aatgtcatga ctcaaaacag
    28501 aatcagcagt taaagaaagg ggaaccgaca gcgcagtgta aggtgcgccg cttattgctt
    28561 gattaaacag tttgatctgt tttctttgtg cttcaacttg cggtgtcgta gagcgtgtat
    28621 atccatacaa agacatattg tcgacacgta cagactcccc cacaatcaac acgtaggtat
    28681 caatacttgt atccctgact gataattgaa aatacggcac cgtgtttgca attgagagta
    28741 atctttgatg ctctttcgct gctaaagcaa aatagttgag attgaaaaac ggcgtatagg
    28801 tagcaaatca cgacgctagt atatatggac tgaacgcttt tttatttttt gcatctttat
    28861 aagcaaattg acaagcggaa aataaactgc ccgaaatgac aatcagcaat aatattcctg
    28921 tcactttttt tgtcggcaag gaaacatcat attttattat tactgccaaa aaaagaaggg
    28981 ataaaaaagc aaaagcacat agataaggga tatacatccc cagcattttc acaacttcat
    29041 ccggatcact ctgcagcaca ctaatcgcaa atccatcatt gaaagttgta ccaaaggtac
    29101 accatgaata gaggctaata ctcatgtctg ccgcagtcag taacacaaat ggtgcggcaa
    29161 caagcaggcg aagcggaagg gatgatatac gtttaacaac caggatggtc aggacgaaga
    29221 acagcaatcg ctgaaccata tgcgctccgg aagaggcgac taagttcgta ataccaaaaa
    29281 caataacaca aagatacaac aaccaatgag atttaaaatc ttctagcaaa tttgttgaat
    29341 actgtcatcc agaaatccaa gttcacaacc aatataaaca attatcaaca atgttcaatt
    29401 ttatatcaga catgtttttc aactctgaac catatcagac cgaaacactg ttagtttttt
    29461 ctttttaaat gatgcgacca ttgcgttctc agtaattaaa taacattatc aatgcgtctc
    29521 taaacattac gcagaacgta gccatcagta gttatactga caggagcagg atggtttatc
    29581 gagaagtaaa catatccgta ttcgtaccgg aactggagtt gacacaataa agtgccaatt
    29641 atgtcagcag tagggaaaag tttataacta aggcgtaccg gcaccatcgt ttcaaggtac
    29701 cagctacgag taaagcaact ggacgagata cagatacctg acaaccattc atcagaccag
    29761 gaccaaaacg aaaagagacg cttttcagcg tctcttttct ggaatttggt accgaggacg
    29821 ggacttgaac ccgtaagccc tattgggcac taccacctca aggtagcgtg tctaccaatt
    29881 ccaccacctc ggcacggata ctacttttta gttcgggata tcgctggtcg gcttagccgg
    29941 agcagctggc tgagtttgtt cggttttcgc cggtgcactc agattttccc attcgctacc
    30001 tctattggtt ttgttgctat tgatgttacc cagcaccaga ctgatgatga agaataacgt
    30061 tgccagcagc gccgtcatgc gggtcatgaa gttaccagaa ccacttgaac caaacagcgt
    30121 agcggaagcg cctgctccga aggaggctcc catatcagcg cctttacctt gctgcagcat
    30181 gatcagacca acaaggccaa ttgccacaat aaggaaaact actaaaagag cttcgtacat
    30241 aatcaacctg ttccttgcgg agttgccgcg taccaatgct tcaaccaata aagcgggagt
    30301 ttttaatctt tcccactgaa gcgggtgtga atactaacca aagcgaatga ccttcgcaag
    30361 ggcaatttta tcgcattgta tcaactgcgg aaaaaaacag caaaaccgat gtgttcgccg
    30421 agaaaaaagg cgacaatcgc cgccttttta gccagttatc taacgcttta aacggctttt
    30481 actgcatcgg cgatgcggtg tgcaaattca gtcacctgcg cttcgtcttc gccttccacc
    30541 atcacgcgaa ttaacggttc ggtgccggat ttacgcagca acacgcgtcc acggttgccc
    30601 agcgcagctt caacctctgc ggtcacggct ttaactgact catgctcaag cggatcgccg
    30661 ctacctgcgg tgtaacgtac gttaaccaga atctgcggga acattttcat gccgctgcaa
    30721 aggtcgtgca ggctcatatg gttacgtgcc atcgccgcca gcacctgcaa gccagcaacg
    30781 atgccgtcac cggtagtggt tttatccagc aggatcacat gaccggaatt ctctgcaccg
    30841 atacgccagc ctttctcctg cattttttcc agtacgtagc ggtcacccac tttcgcgcgc
    30901 gcaaatggaa ttcctaactg tttcagcgcc agttcaagcc ccatgttgct catcaatgta
    30961 cccacagcgc caccacgcag ctggccctga cgaagacctt cacgcgcgat gatatacatg
    31021 atctgatcgc catcgacttt attgccttca tggtcaacca taatcacgcg atcgccgtcg
    31081 ccgtcgaagg caataccgag atccgctttt tcagccagca cacgagcctg gagcgcgcga
    31141 acgtcggtag cccccacttc ggcattgatg tttacaccgt ttggctcaca accgatagcg
    31201 ataacgttcg cccccagttc gcgcagcacg ttcggcgcga tgtgataagt cgcaccgttt
    31261 gcacaatcca ccacaatctt cagttcactg aggctaagtt cgttcgggaa cgtggctttg
    31321 caaaactcga tatagcgacc cgcggcatca acgatacggc tggctttacc cagttctgcc
    31381 gaatcaacgc agctgatctc cttttccatt tccgcttcga tggcctcttc taccgcatcc
    31441 ggcagtttag tgccgtcgat agagaagaat ttaatcccat tatcgtagaa cgggttatgc
    31501 gatgcagata tcacaattcc ggcctctgcg cggaaggtac gcgtcagata agccacggcc
    31561 ggtgttggca tcgggccagt gaagagtgcg gaaaggcccg ctgccgccag acccgcttcc
    31621 agtgctgact ccagcatata gccagaaata cgcgtgtctt taccaataat aatcttacgg
    31681 gagccgtggc gcgccagcac tttacccgcg gcccaaccca gcttaagcac aaaatcaggt
    31741 gtgatcggcg catcccctac acgaccacga atcccatcgg taccgaaata tttacgatta
    31801 ctcatagcgt ttgttttcct ttgcagacag agtggcttcc accacccgca tcgcttctac
    31861 ggtttctttg acgtcatgaa cacgaatgat gtgcgcgcct tgcattgcgg caatgaccgc
    31921 acaggccaga ctaccgctca ggcgctcgga cggccccacg ttcagcagct gcccaatcat
    31981 cgattttcgt gacataccca ccaacagcgg caggttgaaa tggtgaaatt cagccaggcg
    32041 cgccagtaat gaatagttat gggagagatt tttaccgaaa ccgaatccgg ggtcgagcaa
    32101 caatttctct tttgcgatac ccgcctgctc gcaacgtgct atttgctcaa taaagtagcg
    32161 attcacttct gcaaagacat cgtcatactt cggagcttcc tgcatggttt ttggatttcc
    32221 ctgcatatgc atcagacaaa ccggtaaacc ggtttctgca gccgcctcca gagcgccagg
    32281 ttcggaaagg gagcggatat cattaataat gtgagcgcca actttcgctg actcacggat
    32341 gacttctggt ttggatgtat cgaccgagat ccagacttcg aagcgttgag caattgcctc
    32401 aaccacagga ataacacgtt gcaactcttc ttcaacgcta acttccgccg cccctgggcg
    32461 cgtggactcg ccaccaacgt caatgatcgt cgcgccagca ttgatcatca gattcgcatg
    32521 tttcaccgca tctatcagcg agttatgcgt gccaccatcc gaaaaggaat caggcgtgac
    32581 gttgaggatc cccattacgt gaggatggct aaggtccagt gaagtaccct gggcaaagag
    32641 tttcatgatg ttatccctgg tatgaattga taagaaaaaa ccccggagca cgccccgggg
    32701 ttttcggtac aaatacagtc atctgatgcg ggaacttact tgtcgcctaa ctgctctgac
    32761 atggtgttac ccgggttcgg cgtacgcggt tcatcaaccg gacgaggagc ctttggacta
    32821 ccattgtcgc cagaattgtt agaagcgcct ggttcttccc agcccgctgg cggacgtaca
    32881 tcgcgacgtg ccatcaggtc atcaatctgc ggtgcgtcga tagtctcata tttcatgaga
    32941 gcatctttca tcgcatgcag aatatccata ttgtcggtca gaagctgacg cgcacgatta
    33001 tagttacgct caatcagtgc tttcacttcc tggtcgatga tacgtgcagt ttcatcggac
    33061 atatgtttcg ctttcgctac gctacggccg aggaatactt caccttcttc ttccgcgtac
    33121 agcagtggac ccaatttctc agagaagccc cactgagtca ccatgttacg tgccaggttg
    33181 gtcgcaactt taatatcgtt ggacgcaccg gtagaaacat gttccggccc gtagatgatc
    33241 tcttctgcca gacgaccacc gtacagcgta gaaatctggc tttccagttt ctgacggctg
    33301 gcgctgattg cgtcgccctc aggcaagaag aaagtcacac ccagcgcacg accgcgtggg
    33361 ataatcgtca ctttatgcac cggatcgtgt tccggcacca ggcgaccgat aatcgcatga
    33421 cccgcttcgt ggtaagccgt tgattctttc tgcgcttccg tcatcaccat ggagcgacgt
    33481 tccgcaccca tcatgatttt gtctttcgct ttctcgaact caaccatcga cacaacgcgt
    33541 ttgttgccac gagcagcgaa cagtgccgct tcgttcacca ggttcgccag gtcagcaccg
    33601 gagaaaccag gagtaccacg ggcaatgatt gccgcgtcga tatcgggtgc caatggtacg
    33661 cgacgcatgt gaactttcag gatctgctca cgaccgcgaa catctggcaa gccgaccaca
    33721 acctggcggt cgaaacggcc aggacgcagc agggccgggt cgagaacgtc cggacggtta
    33781 gtcgcggcga taacgatgat accttcgtta ccttcgaagc catccatctc aaccagcatc
    33841 tggttcagag tctgttcacg ttcatcgtga ccaccgccca gaccagcgcc acgctggcgg
    33901 cctacggcgt cgatttcatc gataaagatg atgcacggtg ccgctttctt cgcctgttcg
    33961 aacatgtcac gaacacggga tgcacccaca ccgacgaaca tttctacgaa gtcagaaccg
    34021 gagatagtaa agaacggaac tttcgcttcg cctgcaatcg ctttcgccag caacgtttta
    34081 ccggtacccg gaggaccgac catcaggacg cctttcggga tcttaccgcc gagtttctgg
    34141 aagcggctcg gctcgcgcag atactctacc agttcagcaa cttcttcttt tgcttcgtcg
    34201 cagcccgcaa cgtcagcaaa ggtcgttttg atctgatctt ccgtcagcat gcgcgctttg
    34261 ctcttaccaa acgacatggc acctttgcca ccgccgccct gcatttgacg catgaagaag
    34321 atccagacac caatcagcaa cagcatcggg aaccaggaga tgaagataga agccagcagg
    34381 cttggttctt caggcggttc accgacaacc ttgacgttct tggtcaacag gttatccagt
    34441 aatttcggat cctgaaccgg aatgtaagtg gtataacggt tactatcttt cttggtaacg
    34501 ttgatttcac gtccgttgat acgcgcttca cgaacctggt cgttattgac ctcttgtagg
    34561 aaggtagagt aatccacctt acggccatta gactcgctgg gcccaaagct ctggaatact
    34621 gacatcagca caacggcaat gaccagccag agtattaggt ttttcgccat gtcactcaag
    34681 ggattaacct cttattacaa ctgtgttaaa aacagcgtca ggatactcta tatccagcat
    34741 ctttcaaact ttcgtctgaa atctcccggt tagggtttac gcccggtcgc tacaatatac
    34801 acttctcgcg aacgtgcacg agaagagtcc ggcttacgaa ctttgacctt cgtaaacagg
    34861 gagcgaattt cccttagata ctcatcgaaa ccttcgccct ggaacacctt cactacaaaa
    34921 ctgccacctg gcgctaatac atcacgacac atttctagcg ccagttccac cagatacatg
    34981 gcacggggga tatccaccgc cggtgttccg ctcatgtttg gtgccatatc ggacatgaca
    35041 acctggactt tgctgtcgcc aacgcgctcc agcagtgctt tcatcaccag ttcatcacga
    35101 aaatcgccct gaagaaagtc cacaccaacg ataggatcca taggtaaaag atcgcaagcg
    35161 atgatgcggc ctttgccgcc aatttgggtg accacatatt gtgaccaacc acccggagca
    35221 gcaccaaggt cgacaaccgt cattcccggt ttaaagagtt tgtcactttg ctgtatttca
    35281 tcaagtttaa accaggcacg ggaacgtaat ccctttttct gtgcctgttg aacatattta
    35341 tcgctaaagt gttcctgaag ccagcggctg gagctggcag aacgcttctt acctgtcatt
    35401 taactttccc atggggataa ctcatcgtaa ccaattgcgt aaatttttac gcgcctattt
    35461 ggtgatatat gggagatggc ggtagaatga cccgttttca atcccaacgt aagcaaaaaa
    35521 tacgatgaat ctgagtacta aacaaaaaca gcacctgaaa ggtctggcac atccgctcaa
    35581 gccagttgtt ctgcttggca gtaatggttt gaccgaaggg gtgctggccg agattgaaca
    35641 agcgttagag caccatgaac tcatcaaggt gaaaatcgcc accgaagatc gcgaaacaaa
    35701 aaccttgatc gtggaagcta tcgtgcgcga aaccggcgcc tgtaatgtac aggtcatcgg
    35761 taaaacgctg gtgctttatc gcccaactaa agaacgtaaa atctcgctgc cacgctaaga
    35821 ttatcctaaa gttacacaca tttgctgtgt aaaacgaggg gttttccgca ggcaggagag
    35881 caaaatgcca cgctctgttc gttgataaaa ggccgcagag cggccttttt cctttcttta
    35941 caatacatca acatcttgag tattgggtaa ttcttacagg tattccacct taattacttc
    36001 aaattctact tcgccgcccg gcgttttgat gaccacaaca tcatcttctt ctttgccgat
    36061 caggccacga gcaataggcg agtttacaga aatcaggttt tgtttaaagt cagcttcgtc
    36121 atcgccaacg atgcgataag tctgttcttc gtcagaatcc agattcagca ccgttacggt
    36181 agcaccaaaa ataacgcgcc cattgttggg cattttggtg acatcaatca cctgcgcgtt
    36241 tgacagcttg gcttcgatgt ctttgatacg gccttcgcag aagccctgct gttcacgagc
    36301 tgcgtggtat tcggcgtttt ctttcaggtc gccatgctca cgcgcttccg cgatagcagc
    36361 aatgatttca ggacggcgca cagatttcag aaaatccagc tcttcgcgta atttttcagc
    36421 gccgcgtaag gtcatcggaa tagcttgcat ttgttatacc tcttgaatat tcctgatggg
    36481 gcaagtcttc acccaccccg gctgttcggc ctgcccggca taactcctca ccgggaccag
    36541 aagcaaaaaa ataccgaccc gggtacaagt cccaggtcag ctacaattca cattttgatg
    36601 gtcattttac cctgaagttc ccgaagggtc atcgtttact ttatagggcg ttgcgccgta
    36661 gtatgacggc tcgattccag gttgttagcg cgagattatg cgattttcca gatttatcat
    36721 cggattgacc agctgtatag cgttcagtgt tcaggccgcg aatgttgatg agtacgttac
    36781 tcaactcccc gcaggtgcca accttgccct gatggtgcaa aaagtcggtg cgtcggcccc
    36841 cgctattgat taccacagtc agcagatggc gctgcccgcc agtacccaga aagtgattac
    36901 tgcgctggcg gcgttgattc aactcggccc cgattttcgt ttcaccacga cgcttgaaac
    36961 caaaggcaat gtggaaaacg gcatacttaa gggtgactta gtggcgcgat ttggtgccga
    37021 tccgacgtta aaacgtcagg atattcgcaa tatggtcgcg actttgaaaa aatctggcgt
    37081 caaccaaatc gatggcaatg tgttgataga tacctccatt ttcgccagcc acgataaagc
    37141 ccccggctgg ccatggaatg acatgacaca atgctttagc gctccgcctg ctgccgccat
    37201 agttgaccgc aactgtttct ccgtctcgct ctacagtgcc ccaaagcctg gtgatatggc
    37261 ttttatacgc gtggcatctt attaccccgt tacgatgttc agccaggtac gcaccctccc
    37321 ccgtggttct gccgaagcgc aatactgcga actggatgtg gtgccaggcg acctgaaccg
    37381 ctttacgctg acgggatgcc tgccacaacg ttctgagccg ctcccgttgg cttttggcgt
    37441 gcaggatgga gccagctatg ccggtgcaat tctgaaagat gagttaaaac aggcgggtat
    37501 cacctggagc ggaacactgc tgcgccagac tcaggttaac gaacctggaa cggtagttgc
    37561 cagtaaacag tcggccccgc tgcacgatct gcttaagatt atgctgaaaa agtcggacaa
    37621 catgattgcc gatacggttt tccgcatgat cggccatgcg cgcttcaatg tgcctggaac
    37681 atggcgggct gggtcggacg ccgtgcgtca gatcctgcgc cagcaagccg gtgtcgatat
    37741 tggtaacacc attattgccg atggttcagg gctttcgcgg cataacctga ttgcccccgc
    37801 caccatgatg caggtgctgc aatacattgc ccaacacgac aatgaactta actttatctc
    37861 catgctgccg cttgcgggct atgacggctc tttgcagtac cgtgcaggtc tgcatcaggc
    37921 gggcgtggat ggcaaagtct cagcgaaaac cggttcgttg cagggggtat ataacctggc
    37981 gggattcatt accacagcga gcgggcaacg aatggcattt gtacaatatc tttctggcta
    38041 tgcagtaaaa cccgcggatc agcgtaatcg ccgtattccg ttagtgcgtt ttgaaagccg
    38101 tttgtacaaa gatatttatc agaacaatta gtcaaaaaga aaccccggcg catggccggg
    38161 gtttcagatt attgacaaag tgcgctttgt ttatgccgga tgcggcgtga acgccttatc
    38221 cggcctacaa aatcgtgcaa attcaatata ttgcaatcat cttgtaggcc tgataagtgt
    38281 agcgcatcag gcttatttgg cattggtcat tagtgattaa cgcttgtaaa tgaactcaac
    38341 gccttcttcg tcgtcttcgt cccagtcgtc atcccagtct tcatcatcct cttcagcaat
    38401 ctcttcaagc tgctgacggt gataatcatc ccacatgaat tcgactttct ctggctgttt
    38461 cgcttcttca gcctgcacga ccgggttttc aatgataaag gtcatcacat cccagcagag
    38521 atctttcacg cccagtccgc tcgccgcaga gatcagataa tatttatcct cccagcccag
    38581 cgcttcagcg atcgctttcg ctttctcttc ggcttctgcc ttatccagca gatcgatctt
    38641 gttgaagact aaccaacgcg gtttcgccgc cagatcctgg ctgtattttt ccagctcgct
    38701 gataataata cgcgcgtttt caaccggatc ggtgccgtca atcggatcga tatcgatgag
    38761 gtgcaacagg acgcggcaac gttccaggtg cttcaggaag cgaatgccca gacctgcgcc
    38821 ttccgcagcg ccttcaatca gtcctggaat atcggcaaca acgaagctct tttcgttgtc
    38881 cattcgtacc acacccagac ttggcaccag agtggtaaac ggataatccg ccactttcgg
    38941 tttagccgcc gataccgcac gaataaaggt cgatttaccc gcgtttggca tccccaacat
    39001 accgacgtca gccagcagca tcagctccag cagcagctca cgcttatcgc ccggcgtacc
    39061 gttagttttc tgccgtggtg tacggttaac tgacgatttg aaacgggtat tgcccagacc
    39121 gtgccagccg cccttagcaa ccagcagacg ctgaccgtgt ttagtcatat cacccatggt
    39181 ttcaccagta ccctggtcga ttacacgcgt acctaccggc actttaatcg tcacgtcttt
    39241 accgcgctta ccggtacaat cgcggcttgc gccgttctga ccgcgctctg cacggaaaga
    39301 tttttcaaaa cgataatcga taagcgtgtt caggttctcg tcggcttcca tccatacgtc
    39361 accaccatca ccgccgtcgc cgccatccgg gccgcctttc ggaatatact tttcgcggcg
    39421 gaagctcacg caaccattac cgccatcacc tgcaacgacc agaatcgatg cttcatcaac
    39481 aaacttcatt ttattctccg taaatcattc gcctgcgcgg ggttgcgata ccaccgttgt
    39541 atgcttacgt aatccgcccc aaatacgatg accaatggcg gaatacatcg cgcccgcaac
    39601 cacgacaaac gcaccgagat aacctaaaag gtttaacatc ggtctggcga agaaatcggg
    39661 ccaggccagt gataaaagat ctgaaaaaaa cagcgtaaac agtggggtga gcgtgatgat
    39721 cgcgctcacc tgcgctgcct gccagcgagc catcgcttcc gccaaggcgc catatcctac
    39781 caaggtattc agtccgcaaa aaattaaaca tgcgagctgc cagtggctaa gctgcgctat
    39841 cactccaggc tttgccagag ggaagagcgc gattgtacat aaagtgtaca gtaaaaacag
    39901 gatctgcggt gaggccagcc gacgcaataa aaccttttgc gccacgccat aactcaccca
    39961 aaccgtcgcc gcaccgaccc caaagataac tccccaggtg taatcggtga gctttgtaaa
    40021 tatctcgacc agactggtgt taaaaaacat caccagaccg ctcaggagca tcaatgcccc
    40081 tacaacctga gtgctgcgca ttttctcttt caggataaat acgctggcaa ccatcatgcc
    40141 aactggcgag agttgcccaa tcacctgcga agcggtcgga ctcaggtatt gcaaggatga
    40201 gctgaacagg atgaagttcc caaacagccc ggcggtcgcc actgccaaca aaatcaacca
    40261 gcgtggctta cgaaacacgc gtaatggcga caacctcttc ttcaccgcaa gaatggcacc
    40321 aaggccaata ctcgccatca agaaacggta aaacacgatt gtcggaggtt ccatcacctc
    40381 cagcacctgc ttcattgcga ttggcaacgc cccccagcaa attgctgtgg taagcgccaa
    40441 aagaatgcca atgcctgcct gctgcttcat accgtattcc ctgcaaagcg catttgcgga
    40501 ttaacgtcgc accatgcgca tttttaccgg ttatcgaatg taaaaagccc cgcaacgtgt
    40561 tgcggggctt tcatccgtta ccgggacgcg aaaaacttat tcagcttcga tgctgataaa
    40621 tttacggttt ttcgggcctt taacttcgaa tttcacttta ccgtctgctt tggcaaacag
    40681 agtgtggtca cgaccgcaac ctacgttagc gccagcgtgg aatttggtac cacgttgacg
    40741 aacgatgatg ctacccgcca gaacggattc gccaccgaaa cgcttaacgc ccaggcgttt
    40801 agcttctgaa tcgcgaccgt tacgtgtgga gccgccagcc tttttatgtg ccatttgaaa
    40861 tctctcctca ggtcttaggc gctgatgcca gtaattttca catcagtgaa ccactgacga
    40921 tggccctgct gcttacgata gtgtttacga cgacgaaact taacgatttt aactttctcg
    40981 ccacgaccgt gagcaacaac ttcagctttg attacgccgc catcaacgaa aggaacgccg
    41041 attttgactt cttcaccgtt tgcgatcatc agcacttcag cgaactcaac agtttcgcca
    41101 gttgcgatgt ccagcttttc caggcgaacg gtctgacctt cgcttactcg gtgttgttta
    41161 ccaccacttt ggaaaaccgc gtacatataa aactccgctt ccgcgcacac cttttcaatg
    41221 attcagagtg cgctataaat attcacaata gggcgcgaat attacgcaaa acgcacgcct
    41281 ttgacaagtg ctacagtcaa tacacgaaga aaaaaaacac aacttgtacg gtaacgttta
    41341 tctgtgccat tttttcagta caattaccct atattcctaa ccataaaccc taagttgcct
    41401 ttgttcacag taaggtaatc ggggcgaaaa gcccggcttt tgcgatgaat ttagaaaaaa
    41461 tcaatgagtt aaccgcgcaa gatatggcgg gtgttaatgc ggcaatcctt gagcagctta
    41521 attccgacgt ccaactgatc aatcagttag gctattacat cgtcagcggc ggcggtaaac
    41581 gtattcgtcc gatgattgct gtactggctg cacgagctgt tggctatgag ggaaatgcgc
    41641 atgtcaccat tgctgccctg atcgagttta tccacacggc gactctgcta cacgacgacg
    41701 ttgtggatga atcagatatg cgcaggggta aagctaccgc caacgccgca tttggcaatg
    41761 ccgccagcgt gctggtaggc gattttattt atacccgcgc gttccagatg atgaccagcc
    41821 tcggttcact caaagtgctg gaagtcatgt cagaagctgt aaacgtcatc gcagaaggtg
    41881 aagttctgca actgatgaac gttaacgatc cggacatcac tgaagaaaac tacatgcgcg
    41941 ttatctatag caaaaccgcg cgtctgtttg aggctgccgc gcagtgttcc gggattctgg
    42001 ctggctgtac gccggaggag gagaaaggcc tgcaggatta tgggcgctat ctcggcactg
    42061 ctttccagtt gatcgacgat ttactcgatt acaatgccga tggcgaacag ttaggtaaaa
    42121 acgtcggcga cgatctgaac gaaggtaaac cgacgctgcc gctgctgcat gcgatgcatc
    42181 atggcacacc agaacaggca cagatgatcc gtaccgccat cgaacaaggt aacggtcgcc
    42241 atcttctgga accggttctg gaagcaatga acgcttgtgg atctcttgaa tggacgcgtc
    42301 agcgcgccga ggaagaagca gacaaagcca tcgcagcgtt acaggtgctc ccggacaccc
    42361 cttggcgaga agcactcatc ggcctcgcgc acatcgctgt tcaacgcgat cgttaatccc
    42421 ctcccttcat cccgcgcagt gcgcgggatg attccagtaa attccataaa aacactaatt
    42481 cagcgctata acacctgaaa aaaagggcga tgaatcttta tatattctga atattcacac
    42541 tctttacagg aactttttag cgcaataggc catcaggagt atagtgatgc ccgacagaag
    42601 aagtgttctg aatgaaagcg actaacttaa ggagtgagga aagatggaaa gtaatttcat
    42661 tgactggcat cccgctgaca tcattgcagg tttgcgcaag aaaggaacgt caatggcggc
    42721 ggaatctcgc agaaatggtt tgagttcctc aacgctggca aacgcattat cgcgcccatg
    42781 gccgaaagga gagatgatta ttgcgaaagc cctgggaact gacccctggg ttatctggcc
    42841 atcacgctac catgatccgc ggacccatga gtttatcgac agaacgcagt tgatgcgtag
    42901 ctacactaaa ccgaaaaaat gagtggtctg gcggtagccc cgcgaacggg gctgccagct
    42961 ctcagacgat tactcgcctt tcacacgctc aatatttgca cctaaagcgc gcagtttgtc
    43021 ttcaatgcgt tcgtagccac gatcgatgtg ataaatacga tcaaccaccg tcgtcccttc
    43081 cgcaatacag ccagccagca ccaggcttgc tgatgcacgc agatcggttg ccataacctg
    43141 tgcaccagaa agtttttcaa caccatgaca aataacggta ttgctttcga tttcggcgtg
    43201 cgcgcccata cggctcagct ctggcacatg cataaagcgg ttttcaaaga ccgtttcggt
    43261 gatgaacccg gtcccttcag ccaccaggtt caacagcgtg aactgggcct gcatatcggt
    43321 cgggaatgcc ggatgcggcg cggtacgtac gttaacagcc ttcggacgtt tgccatgcat
    43381 atccaggcta atccagtctt cgccgacttc gatgtccgct ccagcatcac gcagtttcgc
    43441 cagcacggcg tccagagtat ctggctgcgc gttacggcag ataattttgc cacgagagat
    43501 cgccgccgcc accaggaaag taccggtttc gatacgatcc ggcagcacgc gatagacacc
    43561 gccgcctaaa cgttccacac cttcgatgac gatacgatcg gtgccctgac cgctaatttt
    43621 cgcacccagc gtaatcagga agttcgcggt atcgacgatt tccggttcac gcgctgcgtt
    43681 ttcaataatc gtggtgcctt ccgccagggt tgcagcacac atgatggtca ccgttgcgcc
    43741 aacgctgact ttatccatca cgatatgtgc gcctttcaga cgaccatcga cggaagcttt
    43801 aacgtaacct tcttccagtt tgatggtcgc gcctaattgt tcgaggccag aaatgtgtag
    43861 atcaaccgga cgagcaccga tcgtacaacc gccaggtagt gaaacttgcc cctgaccaaa
    43921 gcgcgctacc agcggcccca gcgcccagat agaagcacgc atggttttaa ccagatcgta
    43981 aggtgcgcag aatacattaa cgtcgcgggc atcaatatgc acagaaccat tacgttctac
    44041 tttcgcaccc agctggctta gcagtttcat tgatgtatcg acgtctttca gtttcgggac
    44101 gttctggatc tctaccggtt cttccgccag tagtgcggca aaaagaatag gcagagcagc
    44161 atttttagcg ccggaaattg tgacttcgcc ctggagcttc gttggcccct gaacacgaaa
    44221 tttatccatt tagtttgttc tcagttaaca attcatatcc gctaccggcg aatcgcccat
    44281 agctcaaaag ccgttcagtt tgcgatcgcg cgcccactcc gcaggggtat acgctttgat
    44341 cgacacagca tgaatgcggt tatccgcaat atattccatc agcgggccat agaccgtctg
    44401 ctgtttttta acccgactca taccgtcaaa caactcaccc acggcaataa cctgaaagtg
    44461 actgccatcg ccggaaacgt ggacttcctg gagggagaga gcgttcatca acacgctctg
    44521 aatttcatta ttttccatgg gatcttcaat catcagttaa taaaccagcg aaacatctta
    44581 gagcaaagtt gcgctggcat aaataagcaa aaagcctcgc tgataaatca gacaaggctc
    44641 gacttgcagg caggtttgcc ggacaggcgg ttaacgccat atccggcctg aaaaaattta
    44701 acgaggcaga acatcagcag gcaaattata caatttcgcg agggtataca ctttgtcgtt
    44761 taccccctga agcgtcacgt tgttgccctg ctttttcgcc agatcgataa gatggagcag
    44821 cagtgccagt ccccccgtat ccacgcggga cacacggcta agatcgatgc aggtaatccc
    44881 cttcaccgct tcctcacgca tttcccaaag cggtagcaga acgtcctgat ccagctctcc
    44941 ggataacgcc agcgtgtcac ccgtctgcat ccagctcagt gactcgctca ttattttttc
    45001 tcttccagag tgattttctg ttgagaaatc gatttcagtt gcgcagtcag gccgtcgata
    45061 cctttggtac gcagcagcgt tccccactcg ttttgtttgg tggtgatcat actgacgcct
    45121 tcagcaatca tgtcgtaagc ttgccagttt cccgtctggg agtttttacg ccactggaag
    45181 tccagacgca ccggcggacg gccattcggg tcaataatgg taacgcgaat aggcacaatg
    45241 gttttatcgc ccagcggctg ttctggcgca atctgatagg tttgaccgtg atacatcgcc
    45301 agcgcctgac cgtaagcctg cttcaggtac tcacggaaag cggcaaagta ggcttcacgt
    45361 tgagcagggg tcgcactctt gtaatactgg cccagcacca gcgcaccggc gtatttcacc
    45421 tgtacgtatg gcagcagttc ctgatcaaca atggtacgca gataatccgg gttggcccga
    45481 atttgcggtt gctcattctt caggcgatcg aacgttttct gcgccgcctc gtccatcagc
    45541 ttataaggat tggtctggtc tgccgcggtt gccgcactca gaggtgcaat caccagcaaa
    45601 gcgaccatca ttaaacgttt aaacatgcgt cggttctcct gaaattattt cgttgtaccc
    45661 acaggttcag tggtttcatt attacctggc gcagcagctg gcgcatcgcc actattctta
    45721 ttgtcatcgc ctttactacc gtaaaggaac tgaccaatga gatcttccag caccatcgca
    45781 gacttggtgt cctgaattgt atcgccatcc ttcaggatag cagtccccag ttccgggtct
    45841 tcaaaaccga cgtttaatgc cagatattgt tctcccagca ggccggaagt acgaatgctc
    45901 agcgaactgg tatcaggaat gtggttataa cgttgttcaa tttccagcgt tacgcgcggc
    45961 agataggttt tcgggtccag cgtaatatcc gccacccggc ccacaacaac accaccaatg
    46021 ctgaccggag agcgcgcttt caggccgcca atgttatcga acgtcgcata aagcgtgtag
    46081 gtcgattcag tacgtatgga cgtcacgttc gccgccttca ggcaaacaaa cagcgccgcc
    46141 agcagtgctg ctaataaaaa gatacccacc caaatttcat tttttttcgt ttgcatgaac
    46201 tcaattccca aacatcaatg cggtcagcac aaaatccagc cccagaacag ccagagacga
    46261 gtggacaacg gtgcgagtgg ttgcccggct aatcccggca gacgtcggga tggcgtcgta
    46321 gccgttaaac aacgaaatcc acgtcaccgt gatggcgaac accacgctct taatcagaca
    46381 gttgaccaga tccatacgcc agtcgacggc attttgcatt gccgaccaga agaacccgct
    46441 atcaatgcct ttccaactga cgccgaccag cgatccgccc cagatcccca cggcgacgaa
    46501 gataaccgtc aacagtggta atgaaataac cccagcccag aaacggggag aaataacccg
    46561 acgcagcgga tccaccgcca tcatctccat actggagagt tgctctgtag cgcgcatcag
    46621 gccgatttct gcggttaacg ccgaaccagc acgcccggca aacaacagcg cggcaaccac
    46681 cggccccagt tcacgcagta gcgataacgc caccagcata cccagactgg tttccgcgct
    46741 ataagtggtc agaaccagat aaccttgcag ccccaacacc attccgatga acacgccaga
    46801 aactacaata atcagcatcg acaggacgcc gacattatag agctggcgca ccagcagcgg
    46861 cacatgtttg cgaaattccg gtttgccgac cagcgcattg aataacatta acccggcccg
    46921 cccgaacgtt ctcagggttt taatcccttt atgtccgagc gacgccagcg catttaacag
    46981 catgagtggc ttaactccct ggtaaaagat cagcgtgata atcgccggca ggatagcgga
    47041 acggaacagg cccgtcagct atcccgtcca gaaactgacg tacgcgcgga tcaggattcg
    47101 tctgcaacgc ctgggcactg ccatgagcga caattttttt gtccgccagg atccaggcgt
    47161 gatccgcaat acttaacact tccggcacat cgtgagaaac caccacacaa gtcacgccca
    47221 gcgcgctgtt cagctcagaa atcagcttca ccagtacgcc catggtgatg ggatcttgcc
    47281 caacaaaagg ttcatcaaac atgatgagat ccggctccag cgcaatcgca cgcgccagcg
    47341 ctgcacgccg cgccatccca ccggaaagtt cagaaggcat tagtttagcc gctccacgca
    47401 gccccacggc ctccagcttc atcatcaccg tactatgcaa caatggcgcg ggaagttggg
    47461 tatgttcgcg cagtggatag gcgacgttgt caaatacgtt catatcagtg aacaacgccc
    47521 cggactgaaa taacatgctc atccgtttac gcactgtata caggcgcgaa cgagacatcg
    47581 ccggaatatt ctcaccatcg aaaaggatct caccatgatc tggtgcgatt tgcccgccaa
    47641 tcagacggag tagcgtcgtt ttaccgatgc ccgatggccc catgatcgcc gtgatcttcc
    47701 ctcgcggcac ggtcagggaa atattatcga agatgcagcg attgccacgc gtaaaactga
    47761 catcgcgcat atcgactaaa ttcgccacag actgctccat aattcaccct tcgtcttgcg
    47821 ttgattttct aagcatggcg ctcaatttaa ccttgaaccc aacatattta cagaatatta
    47881 cccgccgtgg ttagcgaaag ctggcatttg ttttactttt tagccgcata aagtcaaaat
    47941 taagaatccg ttacggcttt ctgaaaatct tcagcggacc ggcgagtata cctgaagaaa
    48001 ggacgttaga tgcttttagc tacggcactg ttaattgttg gtttactttt ggtcgtttac
    48061 agtgccgacc gcctggtttt tgccgcgtct attctttgcc gaacctttgg catcccgccg
    48121 ctgatcatcg gcatgacggt ggtcagtatt ggtacatcgt taccagaaat catcgtctcg
    48181 cttgccgcgt ctctgcacga acaacgcgat ttagccgttg gtacagccct cggctcaaac
    48241 attatcaata tattgctgct cctcggtctg gctgcgctgg ttcgtccttt taccgtccat
    48301 tccgatgttc tgcgccgtga attaccctta atgttgttgg tcagcgtggt ggccggttcc
    48361 gtactctatg acggacaact tagtcgaagc gatggtatct ttctcctctt tctggctgtg
    48421 ctatggctgc tgttcattgt taaacttgca cgtcagggga ctgacagcct gaccagagag
    48481 cagcttgcag agctgccgcg tgacggcgga ttgcccgtcg cgtttttatg gctcggcatt
    48541 gcgcttatca tcatgccagt ggccacgcgg atggtggttg ataacgccac ggtgctggcg
    48601 aattactttg ccatcagcga gttgacgatg ggtctgacgg caattgctat cggaaccagt
    48661 ctgccggaac tggcaaccgc aatagcgggg gttcgcaaag gtgaaaacga cattgctgtc
    48721 ggaaatatca ttggcgcaaa catttttaat attgtcatcg tgttgggttt acccgcgctg
    48781 ataacgccag gagagattga tccactggcg tacagtcgtg actacagcgt gatgttgctg
    48841 gtgagcatta tttttgcgtt gctgtgctgg cggcgctccc cgcaaccggg ccgtggtgta
    48901 ggggtattat taactggcgg atttatcata tggctggcga tgttgtactg gttatcgcca
    48961 atactcgttg aataactgga aacgcattat gtcgcacgta gagttacaac cgggttttga
    49021 ctttcagcaa gcaggtaaag aagtcctggc gattgaacgt gaatgcctgg cggagcttga
    49081 tcaatacatc aatcagaatt tcacgcttgc ctgtgaaaag atgttctggt gtaaagggaa
    49141 agttgtcgtc atggggatgg ggaaatcggg gcacatcggg cgcaaaatgg ccgcaacgtt
    49201 tgccagcacc ggtacacctt catttttcgt ccatcctagt gaagccgcgc atggtgattt
    49261 aggcatggtc accccacagg atgtggtgat tgctatctct aactcaggtg aatccagcga
    49321 aatcacggcc ttaattccag tgcttaagcg tcttcacgta ccgttaatct gcatcaccgg
    49381 tcgcccggag agcagcatgg cgcgcgccgc agatgtgcat ctgtgtgtta aagtagcgaa
    49441 agaagcctgt ccgttagggc tggcaccgac cagcagcacc accgccacgc tggttatggg
    49501 cgatgccctc gctgtcgcgc tgttaaaagc acgcggcttt actgctgaag attttgcgct
    49561 ctcacaccca ggcggcgcac tgggtcgtaa acttctgctg cgcgtaaacg atattatgca
    49621 tacgggcgat gagatcccgc atgttaagaa aacggccagt ctgcgtgacg cattgctgga
    49681 agttacccgc aaaaatcttg gtatgactgt catttgcgat gacaatatga tgattgaagg
    49741 catctttacc gacggtgatt tacgccgtgt cttcgatatg ggcgtggatg ttcgtcagtt
    49801 aagtattgcc gatgtgatga cgccgggggg aatacgtgtg cgccctggca ttctggccgt
    49861 tgaggcactg aacttaatgc agtcccgcca tatcacctcc gtgatggttg ccgatggcga
    49921 ccatttactc ggtgtgttac atatgcatga tttactgcgt gcaggcgtag tgtaaagatt
    49981 caaggataaa caacaatgag caaagcaggt gcgtcgcttg cgacctgtta cggccctgtc
    50041 agcgccgacg ttatggcaaa agcagagaac attcgtctgc tgatcctcga cgttgatggc
    50101 gtactgtcag atggcctgat ttatatgggc aataacggcg aagagctgaa agcgttcaat
    50161 gttcgtgacg gttatggcat tcgttgtgcg ctcacctctg atattgaagt cgctatcatt
    50221 accgggcgaa aggctaaact ggtagaagat cgttgtgcca cattggggat cactcacttg
    50281 tatcaggggc agtcaaacaa actgatcgcc tttagcgatt tgctggaaaa attggcgatt
    50341 gccccggaaa atgtggctta tgttggcgat gatctcatcg actgcccggt aatggaaaaa
    50401 gtgggtttaa gcgtcgccgt ggccgatgcg catccactgt tgatcccgcg cgccgattac
    50461 gtgacgcgca ttgctggcgg tcgtggcgca gtgcgcgaag tttgcgactt attactcctg
    50521 gcgcagggca aactggatga agccaaaggg caatcgatat gagtaaagcc agacgttggg
    50581 ttatcattgt gctatcactg gcggttctgg tgatgatcgg cattaatatg gccgaaaaag
    50641 acgataccgc ccaggtggtc gtcaacaaca atgatcccac ctataaaagc gagcatacgg
    50701 acacgctcgt ctataaccca gaaggggcac taagctatcg attgattgct caacacgttg
    50761 aatattattc cgatcaggcc gtttcgtggt ttacgcagcc ggtacttacc acgtttgata
    50821 aggataaaat cccgacatgg tccgtaaaag cagataaagc caagctgacc aatgaccgga
    50881 tgctctattt atatggacac gttgaagtca acgcacttgt gccagactct caacttcgca
    50941 gaatcacgac ggataacgcg cagatcaatc tggtgacgca ggatgttacc tctgaagacc
    51001 tcgtcacgtt atacggaaca acatttaact ccagcggtct gaaaatgcgc ggcaacttac
    51061 gcagcaagaa cgccgagctg attgaaaagg ttagaacatc ctatgaaatt caaaacaaac
    51121 aaactcagcc ttaatcttgt gcttgccagc tcacttctgg ccgccagcat tccggcattt
    51181 gccgtaaccg gagacactga tcagccgatc cacattgaat cggaccagca atctcttgat
    51241 atgcaaggca acgtggttac ctttaccggt aatgtcatcg tcacccaggg caccatcaaa
    51301 attaatgccg acaaagtggt cgttacccgt ccgggcggcg aacaaggtaa agaagtgatt
    51361 gacggatacg gtaaaccggc aacgttctac cagatgcagg acaacggtaa acccgttgaa
    51421 ggtcacgctt cccagatgca ctacgaactg gcaaaagatt ttgtcgttct gacgggtaat
    51481 gcttatctgc agcaggtcga tagcaacatt aagggcgata agatcactta cctggtgaaa
    51541 gagcagaaaa tgcaggcttt cagcgacaaa ggcaagcgcg taaccaccgt tctggtgccg
    51601 tcgcagctgc aggacaaaaa caacaaaggc cagaccccgg cacagaagaa gggtaattaa
    51661 ttcgttatgg caacattaac tgcaaagaac cttgcaaaag cctataaagg ccgtcgcgtg
    51721 gtagaagacg tcagcctgac cgtcaactcc ggggaaattg tcggtctgct ggggccaaac
    51781 ggtgccggta agaccaccac tttctacatg gttgtaggca ttgtgccgcg cgatgcgggc
    51841 aacatcatta ttgatgatga cgatatcagt ctgctgcctc tgcatgcacg cgcgcgccgc
    51901 ggtatcggct atctgccaca ggaagcctcc attttccgtc gcctcagcgt ttacgataac
    51961 ctgatggcgg tactgcaaat tcgtgacgac ttgtctgctg aacaacgtga agaccgcgcg
    52021 aacgagctga tggaagagtt tcacattgag cacctgcgtg acagcatggg gcagtcactc
    52081 tccgggggtg aacgtcgccg tgtagaaatt gcccgcgcac tggctgcgaa tccgaaattt
    52141 attctgctcg acgaaccgtt tgccggggtt gacccgatct cggttatcga tattaaacgc
    52201 atcattgaac acctgcgcga cagcggcctg ggcgtgctga tcactgacca caacgtgcgt
    52261 gaaacactgg cggtttgtga acgcgcttat atcgtcagtc aggggcattt gatcgcccac
    52321 ggcacgccta cagaaatctt acaagacgaa cacgttaagc gtgtatacct tggggaagac
    52381 ttcagactct gatagggtag aagtttgcga cgttttagca ggagagtacg attctgaaca
    52441 tgaagcaagg tttgcaactc aggcttagcc aacaactggc gatgacgcca cagctccaac
    52501 aggcaattcg tctgttgcag ttgtcgacgc tggaacttca gcaggagcta cagcaggcgc
    52561 tggagagtaa tccgctgctt gagcaaatcg acactcatga agaaatcgac acccgcgaaa
    52621 cgcaagacag tgaaacgctg gacaccgccg acgcgctcga acaaaaagag atgccggaag
    52681 agctgccgct cgatgccagt tgggacacca tttacaccgc tggtacacca tccggcacca
    52741 gcggtgacta cattgacgac gagctgccgg tctaccaggg cgaaacgacg cagaccttgc
    52801 aggattacct gatgtggcag gtcgagctga caccgttttc cgacactgac cgcgctattg
    52861 ctacctctat cgtcgatgcc gttgatgaaa ccggttatct gactgtcccg ctggaagata
    52921 ttctcgaaag tatgggcgat gaagagatcg acatcgacga ggttgaagcc gtccttaagc
    52981 ggatccaacg gtttgatccg gtcggtgtag cggcaaaaga tctgcgtgac tgcctgctaa
    53041 tccaactctc ccaattcgat aagaccacgc cgtggctgga agaggccaga ttgatcatta
    53101 gcgatcatct cgatctgtta gccaatcacg acttccgcac tttaatgcgc gtcacgcgtc
    53161 tgaaagaaga tgtgctgaaa gaagccgtca atctgatcca gtcgctcgat ccgcgccccg
    53221 ggcagtcgat ccagactggc gaacctgagt atgtcattcc agatgtgctg gtgcgtaagc
    53281 ataacggtca ctggacggta gaactcaaca gtgacagcat tccgcgtctg caaatcaacc
    53341 agcactacgc ctcgatgtgc aataacgcgc gcaacgatgg tgacagccag tttatccgca
    53401 gcaatctgca ggatgccaaa tggttgatca agagtctgga aagccgtaac gatacgctac
    53461 tgcgcgtgag tcgctgtatc gttgaacagc agcaagcctt ctttgagcaa ggtgaagaat
    53521 atatgaaacc gatggtactg gccgatatcg cccaggctgt cgaaatgcat gaatcgacga
    53581 tatctcgcgt gaccacgcaa aaatacctgc atagtccacg aggcattttt gaactgaagt
    53641 atttcttttc cagtcacgtc aataccgagg gcggcggcga agcttcctcc actgcgattc
    53701 gtgcactggt gaagaaatta atcgcggcgg aaaacccagc gaaaccgttg agcgacagca
    53761 agttaacctc tttgctgtcg gaacaaggta tcatggtggc acgccgcact gttgcgaagt
    53821 accgagagtc tttatccatt ccgccgtcaa accagcgtaa acagctcgtt tgacccaacc
    53881 gataaggaag acactatgca gctcaacatt accggaaata acgtcgagat caccgaggca
    53941 ctgcgcgaat ttgttacagc taaatttgcc aaacttgagc aatattttga ccgaatcaac
    54001 caggtctatg ttgttctgaa agtggagaaa gtcacccaca cctcagatgc aacactgcat
    54061 gtaaacggtg gtgaaattca tgccagcgcg gaaggtcagg acatgtacgc tgccattgat
    54121 ggcttaattg ataagctggc acgccagttg accaaacata aagataaact gaaacaacac
    54181 taattgtccg ggcaattagc atgtgcatgg cggcctgttg tgcggcacaa cgggccattt
    54241 gtacggttaa tgctccgagc ctgttccact gtttgagtgg gcaggttctt aggtgaaatt
    54301 atgacaaata atgatacaac tctacagctt agcagtgttc ttaacaggga atgtacgcga
    54361 agccgcgtcc actgtcagag caaaaaacgc gccctggaaa tcatcagcga gctggcggcg
    54421 aaacaactta gcctaccgcc tcaggtggtt tttgaagcta tcctgacgcg ggaaaaaatg
    54481 ggcagtaccg gtatcggcaa tggtattgcc attccgcatg gcaaacttga agaagatact
    54541 ctgcgtgccg ttggcgtttt cgttcagctc gaaacgccta tagctttcga tgccatcgac
    54601 aaccaaccgg tggacttact ttttgccctg ctggtgccgg cagaccaaac taaaacgcac
    54661 ctgcatactc tgtcgctggt ggcgaaacgt ctggcggaca aaaccatctg ccgccgttta
    54721 cgcgcagccc agagcgatga agagctgtat caaatcatta cggataccga aggtactccg
    54781 gatgaagcgt agttattcgg taatgtctct tttagacgtt gtgaggagaa acagtacatg
    54841 gtactgatga tcgtcagcgg acgttcaggt tcaggtaaat ctgtcgccct gcgtgcgctg
    54901 gaagatatgg gtttttactg cgtggataac cttcccgtag tgttgttacc cgatctggct
    54961 cgaactctgg ccgatcgcga gatttctgcc gccgtcagca ttgatgttcg taatatgccg
    55021 gagtcaccag aaatattcga acaggcgatg agtaacctgc ctgacgcttt ctcaccgcaa
    55081 ctactgttcc tggatgccga ccgtaatacc ttaattcgtc gttacagtga cacgcgccga
    55141 ctgcatccgc tttccagcaa aaacctgtcg ctggaaagtg ctatcgacaa agaaagcgat
    55201 ttgctggagc ctctgcgttc gcgagcggat ctgattgtcg atacctcaga aatgtccgtt
    55261 cacgagctgg ctgaaatgct gcgtacccgt ctgctgggta aacgtgaacg cgaactgacc
    55321 atggtctttg agtctttcgg cttcaaacac ggtatcccta tcgatgcaga ttacgtcttt
    55381 gacgtgcgct tcttgccgaa cccgcactgg gatccgaaac tgcgtccaat gacaggtctt
    55441 gataaacctg tcgccgcgtt cctcgaccgc cacacagaag tacacaattt tatctaccaa
    55501 acgcgaagct atcttgagct atggttacct atgctggaaa ccaacaaccg tagctacctg
    55561 acggtcgcca ttggttgtac cggcgggaag caccgttcgg tgtatattgc agagcaactg
    55621 gcagactact tccgctcgcg cggtaaaaac gtccagtcac gccatcgtac gctggaaaaa
    55681 cgtaaaccat gaccgtcaag caaactgttg aaatcacaaa caagctgggc atgcatgccc
    55741 ggcctgcaat gaagctgttt gaattaatgc agggttttga cgctgaagtg ctgttacgta
    55801 atgacgaagg caccgaggct gaagccaaca gcgttattgc gctgctgatg ttggattctg
    55861 ccaaaggacg gcagattgaa gttgaagcga ccggtccaca ggaagaggaa gcactggccg
    55921 ccgttatcgc cctctttaat tctggttttg atgaagatta atcttcatca ctttgacata
    55981 caactccctt caagctcccc cgacaataag aaaatcacgt actgaaatcg ttctcaatca
    56041 acgtcatttg tacattttgt gcgcttttca ctttcagaag aaccttaaga aaaccttaag
    56101 aggcattgtt taggttttgt ttaagttaat cgaccatact ggagatcgtc agaaaatatt
    56161 tccaggagat ggcatgattc gcttatctga acaaagtccc ctgggcaccg gaagacatcg
    56221 gaaatgttat gcgcatccgg aagatgccca acgctgtatc aagattgtct accatcgtgg
    56281 cgatggtggc gataaagaga tccgccgtga gttaaagtac tacgcgcatc ttggtcgccg
    56341 gttaaaagac tggagtggaa taccgcgcta tcacggtacc gttgagacgg attgcggaac
    56401 cggttatgtc tacgatgtga ttgccgattt tgacggcaag ccttccatta cgctgaccga
    56461 atttgctgaa caatgccgtt acgaagaaga cattgcacaa ctgcgccagc tactgaaaca
    56521 gctgaagcgt tatttgcagg ataaccgtat cgtgacgatg tcgctgaagc cgcagaatat
    56581 cctctgtcac cgcatcagtg agtcagaggt tatcccggtc gtctgcgaca acattggtga
    56641 aagcacattg attccactgg cgacctggtc gaagtggtgt tgtctgcgta agcaagagcg
    56701 gttatggaaa cgatttatcg cccaaccggc gctggctatt gccctgcaga aagatctgca
    56761 acctcgtgag agcaaaacgc tggccctcac ttcgcgcgaa gcttaatcca gctggtgttg
    56821 ttgcataaac ggctcaccgc ctaactgata catctgccgt aaaatccacg cctgacggct
    56881 acgcacgtag ccgtcaggcg tggagacttt gaaacgaagt ggattaggta atacagctgc
    56941 cagtaatgca gcttccgacc gggtaagttt gctcgcgggt ttgtggaaat aacgttgtgc
    57001 cgcagcttcg acgccaaaca caccgtcgcc aaattcggcg atattcaggt aaaccgtcag
    57061 gatacgcttt ttgctccaga ctgtttctat ccccagcgtt aatccagctt ccagcccttt
    57121 tcgtacccag ctacgcccat cccataaaaa gagatttttg gccgtctgct gagaaatcgt
    57181 tgaagcaccg cgaatacggt tttcattgcg ctcgttgtgc gccagggctt tctcaatgga
    57241 agcgacatca aagccccagt gctcaggaaa tttctgatct tctgcggcaa tcaccgccag
    57301 tcccatccac ggcgagattt gatccatact gacccagtca gaatgtgcca cgtaacgaaa
    57361 attgccatgc agccaggcgc tgacctgtcg ctcgaccatt accgctgaga agggaacagg
    57421 cgcaacgcta aacaacgcga tgcccccgcc ccagaaaacg gcgaggacaa ccattaaccg
    57481 caaaagaaaa cggcgaacga aactaaacac cgttaagcgg cttttactca ttcagccaga
    57541 accagcacgc gggaaaccag cttatcaatg ccgctcgccg cttctgcaat gttctgcgcc
    57601 agcatatatg ctggggtggt gacaatctta ttgtcttcat caaccacgat atcatcgaca
    57661 ggacacggca catgctccgc gcccatctct tccagtactt ctgcggtatc gatatcagta
    57721 ccgatggtca aacgcagcgg gaaatcgaaa attttcggca gcatcgccgg ggcaatacac
    57781 ataaaaccaa gcggttttcc ggcctgatgc atcgcttgtg ccagcgcctt taattcacgg
    57841 tcaacggtgc attcgctacc aagactggca aaattgctta aattcttcgc cgcgccaacc
    57901 ccccccggca caatcaacgc atccagttca gcggcatcgg cctgggccag aggacggatt
    57961 tcaccgcgcg ttattcgtgc cgcttcaatc agcacattgc gcgtttccgt catcgcttcg
    58021 ccagttaaat ggttgataac atcaacctgc tgcttatccg gtgcaaagca gaccgcctgc
    58081 gcaccgctgc gtgaaatagc taacaacgtc aacaccgctt catgaatttc agaaccgtca
    58141 tagacgccgc atccgctcag aattacgcca attttcttca ttgtgatcat cctttcgcaa
    58201 ttgactgaaa cacattaatt tttttaataa aaatggtacg catcacacat ttaactgatt
    58261 catgtaacaa atcatttaag ttttgctatc ttaactgcgt gcggcctgaa aaacagtgct
    58321 gtgcccttgt aactcatcat aataatttac ggcgcagcca agatttccct ggtgttggcg
    58381 cagtattcgc gcaccccggt ctagccgggg tcatttttta gtggcttttg ccacccacgc
    58441 tttcagcact tctacgtcgt gacgccactc ttctttcatc tcttcaatcc attcaccgac
    58501 gttatcttcc caggcgggaa ggtcaggaga ctgaatttgc tgacccagct gttgcagatg
    58561 gcgtaacccc actgaccccg ccgcaccttt aattttatgt ccttcctcaa caatgccttt
    58621 tttatcctgc gccgtcagat tcgactccag cacgctgaca tagcccggca tcatcttctc
    58681 aaacaccgct aacccgtcgg tgatcagctt cggtcctaca agttcgagat actgttccag
    58741 catgggaata tcgagcaatg cttctgattt actgttctct tctgtcgtca ccgtactctc
    58801 ctcatcatcc tgggtatccc agaatttctt gatcatcgcg gttagcgccg gaaccgaaag
    58861 cggcttactc agcacatcat ccattccagc attgaggtac tcttgtttgt ctttcagcac
    58921 gttagcggtt aaggccacca gcggcggtaa atcctcgcgc ggataacgct tcgtcagttc
    58981 acgagagata tccagcccgg tcatatctgg caactgaata tccagcaaca ccaggtcgta
    59041 ttcgcccggt ttaaacatct ccagcgccgc cttgccggtc atggcgacat caacgctgtt
    59101 acctaatttt tccagcacag aacgcgcgac aatcacgttc agttcaatgt cttccaccag
    59161 cagcacattc agcgccggta aaggcatatc gtcttcatca aacgcatcat cgacctcttc
    59221 tgccaccgac ggtgcgtgga tcgtcaacgt aaaggttgaa cctttgccct gttcgctggt
    59281 aaccgtaata tcgccgccca tatttttcgc cagacgatga gaaacggcca gaccaatacc
    59341 ggtgccggtg gcaggtttac cgccatgact gtctttcacc tggtaataca tggcgaagat
    59401 tttatccagc tcatcctgcg gaatgccgat accagaatct tccacttcaa aatgcagcat
    59461 atcgccttca tcgtagcgca cgcgcacggt aacctggcct tgctgggtga atttgacggc
    59521 gttactgatg aggttccaca ggatctgccg taaacgcgtc ccgtcggtaa tgacctgatg
    59581 cggtaatggc agcgtaggct ccaggttaaa gcgcaatcct ttttgttgcg cctgcaatgc
    59641 ggagagattt tccagatcgg caaggaagct ggtgaaatca accggctgat tatcaagctg
    59701 gaccttgcgc cgttccatct tatccatgtc gataatatcg ttaaagatat tccccagcgt
    59761 gacggccgaa acatggatag ttttgagata tttttcctgc tcggcggtga gttcggtatc
    59821 cagcagaatg cggctcaggc cgacgatacc gttcagcggt gtacgcaatt cgtgactgat
    59881 ggtggaaata aacgtcgttt tgtcgcggct ggcgcgttca agcgcatcct gataccgctt
    59941 acgctcggta atgtcgcgac caaagcccat caaaccgtga cgtttaccca cgcggtcgta
    60001 gtacggcact ttacggattt caaagcaggc tttgcgcccg tccgggtaat ccagccactg
    60061 ttcataggtc agtgacacat tatgacggaa cactttttca tcggtttcaa tgacttttgc
    60121 ggcggcttcc ggtgagtaaa catcagcagg tttcagatga accagttgtt tttcgctttt
    60181 tccggtcagc agctccatcg cgcggttaca gccggaaaac tctttatctt cgttacgata
    60241 aaaaaccagg tcgggtgaag catcaaggaa ggaacgtaag aatgaggatt gctgctcgag
    60301 ctgaatttgt gtctcttcgc gctctttgat ttcaattttc agttggccga aggtttcctg
    60361 cagttctgct tccgcttttt cacgaacggc aatttcctga tttagctggg caatattatc
    60421 ttttaactga acgttgaggc tcaaatcgcg ctcgcgcatc tcctccagtt tttgcaccag
    60481 ccgtgacaga cgttgtcgtg actcctccag ttgctcgacg accaccgata gaaagtagac
    60541 cgcccacggc gtaatcagca aaccaaagaa gatagaacga ataacatcaa tgctttcgac
    60601 ctgaccatgc agcaccatgg ttaccgccat ttgtaccaca atggcaagaa cgacgagggc
    60661 cagcgccagc aacattgaga agcgcaccag acctaacttc atcatcaggt caacataata
    60721 ctgcgccagc agacgaattt gcttcattag ggaattcctt cacgacaacc tgccccaata
    60781 atacccaatt ctgacagtta cgttataaat tgtgtgagaa atgcggaatt cccctcaacg
    60841 acctactccg ttggaggtag ccacggacgt cccagcgccg atccctgtac gccatgttca
    60901 ttcagatagc gatccagctc gaccattccc gtccatcgat tttcgcacca cagcggagca
    60961 agcagcgtcg ggcgacgggc gctggcggaa atgcggtggt agatcacttc cggtggcgta
    61021 tggcgaatca tttctccggc agtgagcgta taatcctcca gttcaatacc gttcaaacgc
    61081 cccgcttccc aggctttcgc cataatgctg cctttcacaa tatgcagcgg atgcagcttt
    61141 atgccgtcca cgctggtttc aaccactcgc tccagcgttt gcaaacattc agcctgccct
    61201 tcgccaggca ggccgacaat taagtgggaa cacaccttca gcccgcgctg acgtgccagc
    61261 tgggttgtac gctgataaca ggcaaaatca tggccgcggt tgatgcgatg cagtgttttg
    61321 tcgtgggcgg tttgtagccc cagctccagc cacacttcgt aaccctggtc cttatattcg
    61381 caaagcagat ccagcaccgc atccggcacg cagtccgggc gggtaccaac acacaaaccg
    61441 acaatattgg cctggctcac cgcctgctga tacatagaac gcagcacctg aacttccgca
    61501 aaggtgctgg tatacgcctg aaagtaggcc agatagcgtt tagcgcggtt aactaaattc
    61561 gcctggtgcg ccagttgctc ggcaatggaa cgatgctgct gcgcttcatc ggcaaacgag
    61621 gcaacattac agaatgtgca gccaccacgc ccgatggtac cgtcacggtt agggcagcta
    61681 aaaccgccgt gcagcgtcag cttatgcacc ttttgcccat aacgacgggt gagatcacca
    61741 ccaaacatat tgactaattt ctgtaactgc ataatctgat agaccgcgcc ttgaaaagag
    61801 gccaaagcct gccattttta gctcaattcg gcgatgacct agatcaatcg tcccggcctg
    61861 cttttatcaa ctgcataatc aatcaaaatt accgaaattt catgcataat cacataaatc
    61921 acttttgctt atcttgtgtc agattttttt atctccttat ggattttagg caaaaacagt
    61981 agcatgaaac gtcattacca attaaggcag tataaaatgc tggttttctc gtcagttcaa
    62041 ggcaggataa gggttaacac gcctttatga cagtcaggaa ttgactgttt ctctaacgac
    62101 tctcctttta gccttaaaga taaaatctat tttaatttca gccatttaat aaagaatttt
    62161 gcgctaaagc acatttctgt accaataagc ttgccatttg acctgtatca gctttcccga
    62221 taagttggaa atccgctgga agctttctgg atgagcagcc tgctcatcat atttatgcag
    62281 taattgagat cccctcttca ccgtattaac cgatgcgaaa aggacaacaa gggggcgaat
    62341 gcgaggcgcg cgtatgacac gcaaaccccg tcgccacgct ctttctgtgc ccgtgcgcag
    62401 cggttcggaa gtggggttcc cgcagagcct gggggaggtt cacgatatgt tgtacgataa
    62461 atcccttgag agggataact gtggtttcgg cctgatcgcc cacatagaag gcgaacctag
    62521 ccacaaggta gtgcgtactg caatacacgc actggcccgc atgcagcacc gtggcgcgat
    62581 tctcgccgat ggtaaaaccg gcgacggttg cggcttgctg ttacaaaaac cggatcgctt
    62641 ttttcgcatc gttgcgcagg agcgcggctg gcgtttagca aaaaactacg ctgtcgggat
    62701 gctcttcctg aataaagatc ctgaactcgc cgctgccgca cgccgcatcg ttgaagaaga
    62761 actgcaacgc gaaaccttgt cgattgtggg ctggcgtgat gtccccacta acgaaggcgt
    62821 gctgggtgaa atcgccctct cctctctgcc acgcattgag caaatttttg tgaacgcccc
    62881 ggcaggctgg cgtccacgcg atatggagcg ccgtctgttt atcgcccgcc gccgcattga
    62941 aaagcgtctc gaagccgaca aagacttcta cgtctgtagc ctgtcgaatc tggtgaacat
    63001 ctataaaggt ctgtgtatgc cggcggatct gccgcgcttt tatctggatc ttgcggacct
    63061 gcgtctggaa tcggccattt gcctgttcca ccagcgcttc tccactaaca ccgtaccgcg
    63121 ctggccgctg gcgcaaccgt tccgctatct ggcgcataac ggtgaaatca acaccatcac
    63181 cggtaaccgc caatgggcgc gtgcgcgtac ctataaattc cagacgccgc tgatccctga
    63241 cctgcacgac gccgcgccat tcgtcaacga aaccggttct gactccagtt ccatggataa
    63301 catgctggaa ctgctgctgg caggcgggat ggatatcatc cgcgccatgc gcctgttagt
    63361 gccacccgcc tggcagaaca acccggatat ggacccggaa ctgcgtgcct tctttgactt
    63421 taactccatg catatggagc cgtgggatgg tccggcgggc atcgtgatgt ccgatggtcg
    63481 cttcgctgcc tgtaacctcg accgtaacgg tctgcgtccg gcacgctacg tcattaccaa
    63541 agacaagctc atcacctgcg cctctgaagt cggtatctgg gattaccagc ctgacgaagt
    63601 ggtcgaaaaa ggccgcgtcg ggccaggcga actgatggtg atcgacactc gcagtgggcg
    63661 tattctgcac tcggcagaaa ccgatgacga tctgaaaagc cgccaccctt ataaagagtg
    63721 gatggagaaa aacgtccgcc gactggtacc gtttgaagat ctgcccgatg aagaagtggg
    63781 tagccgcgaa ctggacgacg acacgcttgc cagctaccag aaacagttta actacagcgc
    63841 ggaagagctg gactccgtaa ttcgcgtact gggcgaaaac ggtcaggaag cggtcggttc
    63901 gatgggcgat gataccccat tcgccgtgct ctccagtcag ccgcgcatta tttacgacta
    63961 cttccgccag cagtttgccc aggtgactaa cccgccaatc gacccgctgc gtgaagcgca
    64021 tgttatgtcg ctcgccacca gtatcggtcg tgaaatgaac gtcttttgcg aagcagaggg
    64081 ccaggcgcac cgtttaagct ttaaatcgcc gattctgctc tactccgatt tcaaacagct
    64141 cacgacgatg aaagaggagc actaccgcgc agatacgctg gatatcacct ttgacgtcac
    64201 taaaaccacg ctcgaagcga cagtcaaaga gctatgcgac aaagccgaaa aaatggtacg
    64261 tagcggcacc gtgttgctgg tgctctccga ccggaatatc gctaaagatc gcctgccggt
    64321 tccggctccg atggcggttg gcgcgatcca gacccgtctg gtcgatcaaa gcctgcgttg
    64381 cgatgccaac atcatcgtcg aaaccgccag cgcccgcgat ccgcaccact tcgccgtgtt
    64441 gctgggtttc ggcgcgacgg ctatttatcc gtaccttgcc tatgaaacgc tgggccgcct
    64501 ggtagacacc cacgcgattg ccaaagatta tcgtaccgtg atgctcaact accgtaacgg
    64561 catcaacaaa ggcttgtaca aaatcatgtc caaaatgggc atctccacca tcgcctctta
    64621 ccgctgctcg aaactgtttg aagcggtcgg tctacacgat gatgtagtgg gcctgtgctt
    64681 ccagggcgcg gtcagccgca ttggtggggc aagctttgaa gacttccagc aggatctgct
    64741 gaatctgtcg aaacgtgcct ggctggcgcg caagccgatt agccagggcg gcctgctgaa
    64801 atacgtccac ggcggcgaat accacgccta caacccggac gtggtgcgca cgctgcaaca
    64861 ggcggtacaa agcggcgagt acagcgacta tcaggaatac gcgaagctgg ttaatgagcg
    64921 tccggcaacc acgctgcgcg atctgctggc aattacgccg ggtgaaaacg cggtcaacat
    64981 tgctgatgtt gaaccggcaa gcgaactgtt taaacgcttt gataccgccg cgatgtctat
    65041 cggcgcgtta agcccggaag cccacgaggc gctggcggaa gcgatgaata gcatcggcgg
    65101 taactcgaac tccggtgaag gcggcgaaga cccggcgcgc tatggcacta acaaagtgtc
    65161 gcgcatcaag caggtggctt ctggtcgctt cggcgttact ccggcgtatc tggtcaatgc
    65221 cgacgtcatt cagattaaag tcgcccaggg cgcgaagccg ggcgaaggcg gtcagttgcc
    65281 gggcgacaaa gtcactcctt acatcgccaa actgcgctat tcggtgcccg gagtgacgct
    65341 gatctccccg ccgccgcacc acgatatcta ctctatcgaa gacttagcgc agcttatttt
    65401 cgacctcaag caggttaacc cgaaagcgat gatctccgtt aagctggtgt ccgaaccagg
    65461 cgttggcacc atcgcgactg gcgtggcgaa agcttatgcg gacttgatca ccatcgcggg
    65521 ctatgacggc ggcaccggcg caagcccgct gtcgtcagtg aaatatgcag gctgcccgtg
    65581 ggaactcggt ctggtggaaa cccaacaggc gctggttgcc aacggcttgc gtcataaaat
    65641 tcgtttgcag gttgatggcg gcctgaaaac gggtgtcgat atcatcaaag cggcgattct
    65701 cggcgcagaa agcttcggct tcggcactgg cccaatggtg gcgctcggct gtaaatatct
    65761 gcgtatttgc cacctgaaca actgcgccac gggtgtagca actcaggatg acaaactgcg
    65821 taagaatcac tatcacggcc tgccgttcaa ggtgactaat tactttgagt ttatcgcccg
    65881 tgaaacccgc gagctgatgg cacagctggg agtaacgcgt ctggtggatc tgattggtcg
    65941 caccgacctg ctgaaagagc tggacggttt caccgccaaa cagcagaaac tggcgctgtc
    66001 gaagctgctg gagactgccg agccacatgc aggtaagtca ctctactgca ctgaaaacaa
    66061 cccgccgttt gataacggcc tactgaacgc gcagttgctg caacaggcga aaccgtttgt
    66121 cgatgaacgc cagagcaaaa ccttctggtt cgatatccgc aacaccgacc gttctgtcgg
    66181 cgcgtcgctt tcaggctata tcgcccagac gcacggcgat caggggctgg cagccgatcc
    66241 tatcaaagcg tacttcaacg gcaccgcagg ccagagtttc ggtgtgtgga acgcgggcgg
    66301 cgtggaactg tacctgaccg gggatgccaa cgactatgtc ggtaaaggca tggcgggcgg
    66361 cttaatcgcc attcatcctc cggttggttc cgccttccgc agccatgaag caagcattat
    66421 cggcaacacc tgcctgtatg gcgcgaccgg tggtcgtctg tatgccgcag gccgcgcggg
    66481 tgaacgtttc ggcgtgcgga actccggtgc tatcaccgtg gtagaaggca ttggcgacaa
    66541 cggctgtgaa tatatgacgg gcggtattgt ctgtattctg ggtaaaaccg gcgttaactt
    66601 cggcgcgggc atgaccggcg gcttcgctta cgttctcgat gaaagcggtg atttccgcaa
    66661 acgcgttaac ccggaactgg tcgaagtctt aagcgtcgac gatctggcga tccatgaaga
    66721 gcatctgcgc ggtcttatca ccgagcatgt tcagcatacc ggttctcagc gcggtgaaga
    66781 gattctggcg aactggtcaa ccttcgccac gaaattcgcg ctggttaaac cgaagtccag
    66841 tgatgtaaaa gcactgctgg gtcaccgtag tcgtagcgca gcagagttgc gcgtgcaggc
    66901 gcagtaaggg gtagcaacaa tgagtcagaa tgtttatcaa tttatcgacc tgcagcgcgt
    66961 tgatccgcca aagaaaccgc tgaagatccg taaaattgag tttgttgaaa tttacgagcc
    67021 gttttccgaa ggccaggcca aagcgcaggc tgaccgctgt ctgtcgtgcg gcaacccata
    67081 ctgcgagtgg aaatgcccgg tacacaacta catcccaaac tggctgaagc tcgccaacga
    67141 ggggcgtatt tttgaagcgg cagaactgtc gcatcagacc aacacactgc cggaagtatg
    67201 cggacgtgtc tgcccgcaag accgtctgtg cgaaggttcc tgcaccctga acgatgagtt
    67261 cggtgcggtg accatcggca acattgagcg ttatatcaat gataaagcgt tcgaaatggg
    67321 ctggcgtccg gatatgtccg gcgtgaagca aaccggtaaa aaagtggcga ttatcggtgc
    67381 aggcccggca ggtctggcgt gtgcggatgt cctgacgcgt aacggcgtaa aagccgttgt
    67441 cttcgaccgt catccagaaa ttggcgggct gctgaccttc ggtattccgg ccttcaagct
    67501 ggaaaaagag gtaatgacgc gtcgccgtga aatcttcacc ggcatgggta ttgaattcaa
    67561 actcaatacc gaagtgggcc gcgacgtaca gctggacgat ctgctgagtg attacgatgc
    67621 cgtgttcctt ggcgttggga cttatcagtc aatgcgcggc gggctggaaa acgaagacgc
    67681 cgatggcgtg tacgcagcgc tgccgttcct tatcgccaac accaaacagt taatgggctt
    67741 tggtgaaacc cgcgacgaac cgttcgtcag catggaaggc aaacgcgtgg tagtacttgg
    67801 cggtggcgac actgcgatgg actgcgtgcg cacgtctgta cgccaggggg caaagcacgt
    67861 tacctgtgcc tatcgtcgtg atgaagagaa catgccgggt tcccgccgcg aagtgaaaaa
    67921 cgcgcgggaa gaaggcgtag agttcaaatt caacgtccag ccactgggga ttgaagtgaa
    67981 cggtaacggc aaagtcagcg gcgtaaaaat ggtacgtacc gaaatgggcg aaccggatgc
    68041 caaaggccgt cgccgcgcgg agatcgttgc aggttccgaa catatcgttc cggcagatgc
    68101 ggtgatcatg gcgtttggtt tccgtccaca caacatggaa tggctggcaa aacacagcgt
    68161 cgagctggat tcacaaggcc gcatcatcgc tccggaaggc aacgacaacg ctttccagac
    68221 cagcaacccg aaaatctttg ctggcggcga tatcgtccgt ggttccgatc tggtcgttac
    68281 cgccatcgcc gaaggtcgca aagcggcaga cggcattatg aactggctgg aagtttaagc
    68341 tctgtcccag cgccgcctcc tcagaaaaga gtcggcgctg aaattctcct ttctttctgg
    68401 aaatcgcctc gcataatctg aagaaaacca atgtaatacg ctcatctgga ttgttaccgg
    68461 tagatgcccg gcgtatagct gattccatta ttcaacgcag cgccatataa agggacagat
    68521 aatggaatcg ctctcagaga gaacctcaac aggctaccag caaatccacg acggcattat
    68581 tcatctggtc gatagcgccc gaacggaaac ggtacgtagc gttaacgcgc tgatgaccgc
    68641 gacgtactgg gaaattggcc gacggattgt cgaatttgaa caaggtggtg aggccagagc
    68701 agcttatggt gcacagttga ttgatcggct atcgaaggat ttaagtcgac ggtataaacg
    68761 tggggtctct gcaaaaaact tacgccaaat gaggcttttt tacctctttt ttcaacatgt
    68821 tgaaattcgc cagacagtgt ctggcgaatt aacaccattg ggaattccgc agacaccatc
    68881 tgcagaattc ccatcggcaa agatttggca gacgctgtct gccaaattga gaggctgttt
    68941 gatatttcca cagcttttgt attgcatctc ctattttcgg tttgtatatt catgcaaa
//
LOCUS       DAJTYG010000006        67702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTYG010000006 DAJTYG010000000
VERSION     DAJTYG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.006"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..247
                     /locus_tag="OO568_000455"
     CDS             <1..247
                     /locus_tag="OO568_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6639945.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="OO568_000456"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="OO568_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6639946.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="OO568_000457"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="OO568_000457"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6639947.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="OO568_000458"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="OO568_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR6639948.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTMLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMVNVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="OO568_000459"
     CDS             complement(3258..3542)
                     /locus_tag="OO568_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639949.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="OO568_000460"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="OO568_000460"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6639950.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMKNAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="OO568_000461"
     CDS             complement(4443..5651)
                     /locus_tag="OO568_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6639951.1"
                     /translation="MTVNSSRNALKRRTRALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAAAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /gene="rcdA"
                     /locus_tag="OO568_000462"
     CDS             5735..6271
                     /gene="rcdA"
                     /locus_tag="OO568_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6639952.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="OO568_000463"
     CDS             complement(6446..8131)
                     /locus_tag="OO568_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6639953.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAITNVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="OO568_000464"
     CDS             8401..8778
                     /locus_tag="OO568_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6639954.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="OO568_000465"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="OO568_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6639955.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="OO568_000466"
     CDS             9225..9512
                     /locus_tag="OO568_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6639956.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="OO568_000467"
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="OO568_000467"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR6639957.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="OO568_000468"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="OO568_000468"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6639958.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="OO568_000469"
     CDS             11269..11745
                     /locus_tag="OO568_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6639959.1"
                     /translation="MTSLVVPGLDTLRQWLDGLGMSFFECNNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="OO568_000470"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="OO568_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6639960.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14380
                     /gene="potG"
                     /locus_tag="OO568_000471"
     CDS             13304..14380
                     /gene="potG"
                     /locus_tag="OO568_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6639961.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPT"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="OO568_000472"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="OO568_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6639962.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="OO568_000473"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="OO568_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6639963.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="OO568_000474"
     CDS             16302..16790
                     /locus_tag="OO568_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6639964.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="OO568_000475"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="OO568_000475"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6639965.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="OO568_000476"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="OO568_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6639966.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="OO568_000477"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="OO568_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6639967.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="OO568_000478"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="OO568_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6639968.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="OO568_000479"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="OO568_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6639969.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="OO568_000480"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="OO568_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6639970.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="OO568_000481"
     CDS             complement(22242..22757)
                     /locus_tag="OO568_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6639971.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="OO568_000482"
     CDS             23406..25175
                     /locus_tag="OO568_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6639972.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSIGARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="OO568_000483"
     CDS             25386..25709
                     /locus_tag="OO568_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6639973.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /gene="amiD"
                     /locus_tag="OO568_000484"
     CDS             25706..26536
                     /gene="amiD"
                     /locus_tag="OO568_000484"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6639974.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="OO568_000485"
     CDS             complement(26533..27546)
                     /locus_tag="OO568_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6639975.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIAHSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="OO568_000486"
     CDS             complement(27645..29075)
                     /locus_tag="OO568_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6639976.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="OO568_000487"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="OO568_000487"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6639977.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGAHIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="OO568_000488"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="OO568_000488"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6639978.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="OO568_000489"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="OO568_000489"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6639979.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="OO568_000490"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="OO568_000490"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6639980.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPSFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="OO568_000491"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="OO568_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6639981.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="OO568_000492"
     CDS             complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="OO568_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR6639982.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            37227..38885
                     /locus_tag="OO568_000493"
     CDS             37227..38885
                     /locus_tag="OO568_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6639983.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(38882..39838)
                     /locus_tag="OO568_000494"
     CDS             complement(38882..39838)
                     /locus_tag="OO568_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6639984.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            39989..41104
                     /gene="macA"
                     /locus_tag="OO568_000495"
     CDS             39989..41104
                     /gene="macA"
                     /locus_tag="OO568_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6639985.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMGGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            41101..43047
                     /gene="macB"
                     /locus_tag="OO568_000496"
     CDS             41101..43047
                     /gene="macB"
                     /locus_tag="OO568_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6639986.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(43120..43344)
                     /gene="cspD"
                     /locus_tag="OO568_000497"
     CDS             complement(43120..43344)
                     /gene="cspD"
                     /locus_tag="OO568_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6639987.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            43667..43987
                     /gene="clpS"
                     /locus_tag="OO568_000498"
     CDS             43667..43987
                     /gene="clpS"
                     /locus_tag="OO568_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6639988.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            44018..46294
                     /gene="clpA"
                     /locus_tag="OO568_000499"
     CDS             44018..46294
                     /gene="clpA"
                     /locus_tag="OO568_000499"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6639989.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(46697..46784)
                     /locus_tag="OO568_000500"
     tRNA            complement(46697..46784)
                     /locus_tag="OO568_000500"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46748..46750),aa:Ser,seq:gga)
     gene            complement(47038..47256)
                     /gene="infA"
                     /locus_tag="OO568_000501"
     CDS             complement(47038..47256)
                     /gene="infA"
                     /locus_tag="OO568_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6639990.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(47541..48245)
                     /gene="aat"
                     /locus_tag="OO568_000502"
     CDS             complement(47541..48245)
                     /gene="aat"
                     /locus_tag="OO568_000502"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6639991.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVCCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(48287..50008)
                     /gene="cydC"
                     /locus_tag="OO568_000503"
     CDS             complement(48287..50008)
                     /gene="cydC"
                     /locus_tag="OO568_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6639992.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(50009..51775)
                     /gene="cydD"
                     /locus_tag="OO568_000504"
     CDS             complement(50009..51775)
                     /gene="cydD"
                     /locus_tag="OO568_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6639993.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(51898..52863)
                     /gene="trxB"
                     /locus_tag="OO568_000505"
     CDS             complement(51898..52863)
                     /gene="trxB"
                     /locus_tag="OO568_000505"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6639994.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            53408..53902
                     /gene="lrp"
                     /locus_tag="OO568_000506"
     CDS             53408..53902
                     /gene="lrp"
                     /locus_tag="OO568_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6639995.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            54037..58143
                     /gene="ftsK"
                     /locus_tag="OO568_000507"
     CDS             54037..58143
                     /gene="ftsK"
                     /locus_tag="OO568_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6639996.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAVDPDDVLFLGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSIFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFTQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            58298..58909
                     /gene="lolA"
                     /locus_tag="OO568_000508"
     CDS             58298..58909
                     /gene="lolA"
                     /locus_tag="OO568_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6639997.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDEQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            58920..60263
                     /gene="rarA"
                     /locus_tag="OO568_000509"
                     /gene_synonym="mgsA"
     CDS             58920..60263
                     /gene="rarA"
                     /locus_tag="OO568_000509"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6639998.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            60354..61646
                     /gene="serS"
                     /locus_tag="OO568_000510"
     CDS             60354..61646
                     /gene="serS"
                     /locus_tag="OO568_000510"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6639999.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            61886..64330
                     /gene="dmsA"
                     /locus_tag="OO568_000511"
                     /pseudo
     CDS             61886..64330
                     /gene="dmsA"
                     /locus_tag="OO568_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            64341..64958
                     /gene="dmsB"
                     /locus_tag="OO568_000512"
     CDS             64341..64958
                     /gene="dmsB"
                     /locus_tag="OO568_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6640000.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            64960..65823
                     /gene="dmsC"
                     /locus_tag="OO568_000513"
     CDS             64960..65823
                     /gene="dmsC"
                     /locus_tag="OO568_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCR6640001.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(65859..66485)
                     /locus_tag="OO568_000514"
     CDS             complement(65859..66485)
                     /locus_tag="OO568_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6640002.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            66800..>67702
                     /locus_tag="OO568_000515"
     CDS             66800..>67702
                     /locus_tag="OO568_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6640003.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGSVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFT"
BASE COUNT        16824 a        16760 c        17761 g        16357 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttacactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccatg ttgcgcttct tgcagggcat
     2341 aagcctctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggt gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccac
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattggag cctgcccggt attttgccgc
     3721 tgcgacgacc gcgctgccgg catttttcat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcactac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatact
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcggc ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta acggtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttt gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgccctg gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaagc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggtga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg tgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tctgccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggtaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgttacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg gcctggggat gagttttttt
    11341 gaatgtaata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 cttgaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggtgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gcgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctag ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagcagtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg atccgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcctggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 agacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggcaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca ccaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaactggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg aggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tatgaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tggatgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatgctttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg ttttattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atcggtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata atcgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcca gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gtgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat cgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccacct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgccagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gtgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcgt cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaaca caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    32401 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgcat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gctcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaactaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 tttcctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggt taagacgctt aacgcgggga taaaagggat
    34681 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gatctaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaataact tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aaggacgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg
    35761 atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat
    35821 aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc
    35881 cgggctctgg cgagcaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac
    35941 caggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct
    36001 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt
    36061 aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa tcacgctttt
    36121 ccagcagagt ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga
    36181 agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg
    36241 ccgggttaac agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca
    36301 gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca
    36361 ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac
    36421 cgcctggtga atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac
    36481 ccgttttacc actggcaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct
    36541 gggcaatcac gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa
    36601 tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca
    36661 gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc
    36721 ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt
    36781 cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt gaaaaatatg
    36841 gtcgttttat agggtcgtcg taaaaaatga cgacggaaat aatgcgcggc tattttaaaa
    36901 acgaaggcga gtcattcacc agataaataa atccagtaaa tttgatttag ggcaacagcg
    36961 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcaagcaaaa gatttaatcc
    37021 tttaggcgta ataaaaaata atttatcatg ctaattattt gattttgttg tttttgcggg
    37081 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca attgccatgc
    37141 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
    37201 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
    37261 gcggtatcaa ccgtttgtcg ttgatgctcg aacaaaacaa cgtcctgatt ggggagaacg
    37321 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
    37381 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
    37441 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
    37501 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
    37561 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
    37621 ataaagacgg acatccgatt gatgttgagg atattaacga tcaggcacgc catctggtgc
    37681 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
    37741 cggtgccaaa tgtccccaat gtggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
    37801 agttgtcctc acatccgcaa aatctctctg atggacagat tcgtcaggga ctttccgcaa
    37861 tggtgcagtt gcttgagcat tatttctctg agcagggggc tggacaggcg cgatatcgtt
    37921 taatgcggcg gcgggccagc aatgagcaac gaagctggcg ctatctggac atcatcaacc
    37981 ggatgattga ccgacctgga gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
    38041 ctttattgca ggcaaaaggc acattgcgac tggataaaga cgcgcgtccg ttattgctaa
    38101 ttgaagatcc agaaacccgc ctgcatccga ttatgctttc cgttgcctgg catctgttga
    38161 atcttttgcc tttacagcgt attgctacca ccaactctgg cgagctgctt tcgttaactc
    38221 cagtggaaca tgtttgccgc ctggtgcgtg aatcttctcg tgttgccgcc tggcgtctgg
    38281 ggccaagtgg cttgagcacc gaagacagcc gccgcatctc ttttcacatt cgttttaatc
    38341 gtccgtcatc gctgtttgct cgctgctggt tgctggtgga aggggaaacg gaaacctggg
    38401 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
    38461 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
    38521 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
    38581 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
    38641 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
    38701 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
    38761 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
    38821 taccaacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
    38881 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
    38941 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
    39001 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgactcc cagaacgcgt
    39061 tgtaatcagc atggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
    39121 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
    39181 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
    39241 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
    39301 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgga
    39361 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
    39421 actgctcacc gtttttgcct tccagtttag ccagttgcag cccgggggta ttcaaatata
    39481 agctgaattc ttccgccgac atacacccgc ggagtaacgc ataatggtaa cgtaacgcct
    39541 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
    39601 gtcgcggctg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
    39661 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
    39721 agcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
    39781 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
    39841 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
    39901 tgtgattact aacgtaaatt ttaggatttt gttgatattt cgttgaagtt aatgacccgg
    39961 attggcatgt ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
    40021 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
    40081 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
    40141 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgta ggcgcgcagg tcagcggtca
    40201 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
    40261 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
    40321 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
    40381 tcagcaacgt ctggcacaaa cgcaagcggt ttcactgcag gatctcgata ccgccgcgac
    40441 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
    40501 ggcctctctc gatacggcta aaaccaatct cgattacacc cgtatcgttg ccccgatggg
    40561 cggggaagtg acgcaaatca ccactctgca aggccagacg gtgattgccg cacagcaagc
    40621 accgaacatt ctgacgctgg cagatatgag cactatgctg gtaaaagcgc aggtttctga
    40681 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtgc ttggcgatcc
    40741 actgacgcgc tacgaggggc aaatcaagga tgtgctaccg acgccggaaa aggtaaacga
    40801 cgctattttc tattacgccc gttttgaagt acccaacccc aatggtttgc tgcggctgga
    40861 tatgaccgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgttga cgatccctct
    40921 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
    40981 tgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
    41041 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
    41101 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg tgatgagcag
    41161 gttgaggtgc tgaagggcat cagcctcaat atttatgcgg gtgagatggt cgcgattgtt
    41221 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
    41281 accagcggca cctatcgcgt agccggtcag gatattgcca cgctggacgc cgatgcgctg
    41341 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
    41401 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ccggtcttga gcggaaacag
    41461 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
    41521 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
    41581 aacggcggtc aggtaattct tgccgatgaa ccgaccggcg cactggacag ccattctggc
    41641 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
    41701 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
    41761 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
    41821 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
    41881 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
    41941 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
    42001 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
    42061 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
    42121 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
    42181 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
    42241 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
    42301 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
    42361 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
    42421 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
    42481 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
    42541 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
    42601 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
    42661 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggcgg tattggtgta
    42721 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
    42781 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
    42841 ctggtcggtg gcgcgttggg aataacactg tcactgttaa ttgctttcac cttgcagctt
    42901 ttcttacccg gctgggagat tggtttttca ccgttggcgc tgctgctggc gtttctctgc
    42961 tcgacggcca ccgggatttt atttggctgg ttacccgcac gaaatgcggc acgactggat
    43021 ccagtagatg ctctggcacg ggagtaattt ttgagataaa aatgccagcc gatcgggctg
    43081 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
    43141 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
    43201 aacggattgt ccagctttta gcgttctgta accatccatc tgaatggtgg aataatgagc
    43261 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaacctttgg cattgttgaa
    43321 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
    43381 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
    43441 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
    43501 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
    43561 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
    43621 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
    43681 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
    43741 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
    43801 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
    43861 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
    43921 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
    43981 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
    44041 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
    44101 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
    44161 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
    44221 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
    44281 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
    44341 tctggtcgct atcttcagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
    44401 agtcagccgt ctcgatgtgg tgaactttat ctctcatggc acgcgtaaag acgagccgac
    44461 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
    44521 tatggagaat ttcacgacga acctgaacca gcttgcgcgt gtgggcggaa tcgacccact
    44581 aattggtcgt gagaaagagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
    44641 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcag aaggtcttgc
    44701 ctggcgaatt gttcagggtg atgtgccgga agtgatggct gactgtacga tttactctct
    44761 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
    44821 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
    44881 caccattatc ggtgcgggtg cagcgtctgg tggtcaggtc gatgcggcta acctaatcaa
    44941 accgttgctc tccagcggta aaattcgcgt aattggttcg acaacctatc aggagttcag
    45001 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
    45061 accgtcgatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
    45121 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
    45181 cattaacgat cgtcatctgc cggataaagc cattgatgtt atcgacgaag cgggcgctcg
    45241 cgcacgtctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
    45301 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
    45361 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggccaggata aagccattga
    45421 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
    45481 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
    45541 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
    45601 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
    45661 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
    45721 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
    45781 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ctaacgccgg
    45841 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
    45901 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
    45961 ctggttcgat catctgtcaa ctgacgtgat ccatcaggtg gtggataaat tcatcgtcga
    46021 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
    46081 ctggctggcc gagaaaggtt acgaccgggc aatgggcgca cgtccgatgg cgcgtgtcat
    46141 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
    46201 cggtcaggtg acggttgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
    46261 tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat tgtcaggtag gttggtgaag
    46321 tccgtaatct cgtcaggggt tacggacttt ttatttatgg ggtggaacgg tgaaaaccct
    46381 atttttggag gtgaaggtaa gttgttgata attagtgctg ctggaaggta aggataaaaa
    46441 agggtgctgc aggagaatgg gatggttttg ctttattcac aacgggctaa acgtgtagta
    46501 tttgagttca ctgccgtaca ggcagcttag aaatcgacgg ggtgcggtaa aacctttgcg
    46561 aacgcgttca ctgccgtaca ggcagcttag aaattcacag gtaacatact ccacccgccc
    46621 accatgttca ctgccgtaca gacagataaa atgcgaaaaa aaagctcgca ctttcgtacg
    46681 agctcttctt taaatatggc ggtgaggggg ggattcgaac ccccgatacg ttgccgtata
    46741 cacactttcc aggcgtgctc cttcagccac tcggacacct caccaaattg ttttgctacc
    46801 aaacctcatg ggtggcaacg gggcgctact atagggagtt ggagtaaaac ggtcaagaag
    46861 aattttaatg ataattattg tttgctcata ctgtaaacaa cttgtgcagt atatctacat
    46921 cgagacaagt tatggactta tacttccaaa gtacttcata catatcacaa aataaaaagg
    46981 ccggttaaac cgacctttta ctcgttcttt ctcttcgccc atcaggcggt aaaacaatca
    47041 gcgactacgg aagacaatgc ggcctttgct caggtcgtac ggggtcagtt caacagtcac
    47101 tttgtcgccc gtcaggatgc ggatgtagtt tttgcgcatt ttaccggaga tgtgtgcagt
    47161 aaccacgtga ccgttttcta actctacgcg gaacatggta ttaggcaacg tttcaagaac
    47221 ggtaccttgc atttcaatat tgtcttcttt ggccatctaa tcctctgggg tatcactacc
    47281 gtaatttgaa ccggcaagat aatgccgaag ttctgtaaat aagtaaagat ttgcgcgcta
    47341 aatcgcaaca aacaggttcg gcacattact ccgaaaacac acggctaagc cgcaccaaaa
    47401 gcgcaacgta taagggagcg gtgagataaa cgatgggcgt tacctgacgc gaaaaattcc
    47461 ttatcggcag cggggtaatg agcgtaacca actctgcgac cgcaattata acactctggg
    47521 gagaaatgtg ccgaaaacat tcattcttgt ggtgaaaaca agcaccgtgg tacccagaaa
    47581 ttattcggca atcgtccgag gcgcatttga ttgagataat taaggtaatc ccggcgggga
    47641 atttcgcagg caccaagcga tgctgtgtga tcgttaagga cctggcagtc gataagctta
    47701 ccgccatgac cgataaattc ctcacagcat accagaagcg ccgttttaga cgcattttcc
    47761 atccggctga acatggactc gccacaaaat agcgttccct gggccacgcc gtacataccg
    47821 ccgacaagct catcttcacg ccagacttca atggagtggg catgaccgag ttcgtgaagg
    47881 cgatggtagg cttcgaccac gccacgcgtg atccaggttc cttcttcgcg atcgctggca
    47941 cagccttcaa tgacctgacc aaaagcgtaa ttcatcgtga cacgataggg cgagcgttta
    48001 tgaaatcgct tcatactacg gctgatatgc agtgattctg gccatagcac cgcgcgggga
    48061 tcgggcgacc accagaggat ggggtcgcct ggagaaaacc acggaaaaat accacgctgg
    48121 taagccatta acaggcgcgc agggctaaga tcgcccccaa gtgccagcag gccgttaggc
    48181 tcacgtaatg cgccttccgg ggaagggaag gctattgaat ggcgagaaag ctgaaccagg
    48241 cgcatgaccg caaaactcca cgcaagtcgg atcgttcaat aatagcttac aaaccctgct
    48301 tgaactggta ataacgcccc tgtctggcaa gcagttctgc gtgagtacct tgctcaataa
    48361 tttgcccgtt gtccatcact attatttgtt ggaaacgaga gagtccgcga agtcgatggg
    48421 tgaccattaa caccgttttc tcacgcatca tttttgcaag caattcaagg atctggcttt
    48481 cggttgtggc atctaagcct tcggtaggtt catccagcaa caccagtggc gcatcatgta
    48541 acagcgcacg ggcgatagcc agacggcgca gttcaccacc ggagagctgg cgtccgcctt
    48601 cacctaacca actgttgaga cctgcatcct cgagcagctt ctccagacca acgcgacgca
    48661 agatctccga cagagcctca tcactactgc caggcgaggc gagtaacaga ttatcacgca
    48721 gcgtggcgct aaacagatgc actcgctgag gaacaacgct gatggtctgt cgtagagccg
    48781 cttcattcag gctggctatg gggctatcgt taagcaaaat ctcgccctgt tgcgggtccc
    48841 atgcgcgggt cagcagttgt aacagtgttg atttgccgca tccggttcgc ccgagaatcg
    48901 ctatatgttc cccggcgttt acctgaagag aaatcccttt aagtgcctgt tgggattgct
    48961 ccggataagt gaactgaaca tcccgtaatg tcagcgaaac gcgatcggca acacgagttt
    49021 gggtatcagg gaaggtgacc tccggttttt gatccgttaa gtcagagata cgtacggcag
    49081 aggcaatgac ttgccccaga tgctgaaatg cacccgttac tggtgccagt gcttcaaacg
    49141 cggctaacgc acaaaagaca aacagggcaa ttaatgcgcc gggttgagca ttgccgccaa
    49201 cgccgccaga cgccatccac agcatcagga tcaccgctaa cgcgccaatg agcagcatta
    49261 tcgcttgcga caatgcggtc agttcagatt gacggcgttg cgcttccagc cattgaattt
    49321 ctgtattctc tagttgcgtg cgataacgat cgctggcacc aaaaatggtc agctcagctt
    49381 gcccttgcag ccaggccgtc agttgttggc gatactgtcc gcgaagatga gtcagatttt
    49441 gcccggtgct ttttcccgca cgataaaaca gcggtggcat caggaaaagc gtcagtaaca
    49501 taacgctgcc cagcgtaaag gcgagggtga aatcaaggaa acttaacccg attgtcacca
    49561 ccataatcac cacaaaagcg cccaccagcg gcgagataac gcgcaggtaa agatgatcga
    49621 gcgtatcaac atccgccacc acgcggttga gcaattcgcc ctgacgatag cgcgccagtc
    49681 cggcagggga gaggggcaac aatttgctga aggtgtaaat gcgcagatgc tgcaacacgc
    49741 ggaaagtcgc gtcgtgactt accagacgtt caaaataacg cccggcagta cgggtgattg
    49801 cggcaccacg cacgcccgca gcgggtagca tatagttgaa gctgtacagt ccggcaaccc
    49861 ccgcaaccgc tgaggccgag aggaaccagc cggaaagtgt caacagaccg atactggcga
    49921 gcagcgtcac aattgccagc acaataccaa gacttaacat ccatttatga cgtttataca
    49981 gtgccagata gggtagcaaa gcgcgcattt aaatctcctc ctgacgatgg gccagtaatg
    50041 tggcgaatgg gccaccagcc acacttaatt ccgcgtaacg tccttgctca ataatccggc
    50101 catcctgcat aacccaaatg acatcccagt cagcaagatc ttctaactgg tgggtgacca
    50161 ttaacgttgt ctggcgcaga gaggcggcat tcagcgcctc cattacgcgc tgttcactgt
    50221 gagcatcaag gctggcagcg ggttcatcca acagtaatag cgaacaggga tttagtaacg
    50281 cacgggccac cgccacgcgc tgcgcctgcc ccacggaaag gcgggcagcc tggtcgccaa
    50341 caggtgtatc aacgccttgt ggcaggagcg gtagaaactc gctgacccag gcgttatcca
    50401 gcgctgcttg taattcttgt tcgctggcat caggtcgcgc cagtagtacg ttatcccgca
    50461 attttgctgc cggtaattgt gggttttgcc caacccagga gagatgttta cgccatgatt
    50521 ctggtgataa atcacgtaat tctatcccgt tgattcgtaa tgatccctga tatgagagaa
    50581 aaccagaaag cgcgttcagc agtgagcttt tacctgaacc gctgcggcca accaacactg
    50641 cacgttggcc tgctggcaaa gtaaagttca gcggtccggc cagcgtttta ccttccggcg
    50701 acgtgataaa cagatcctcg gcctcaatgg tcagcggatc ggtcaatgct aattccgcct
    50761 caccgcgctg cggatgggcg agcggggttt ccataaacgt tttcagactg tcagctgcac
    50821 caacagcctg ggctttagca tgataaaacg taccgagatc gcgtaatggc tggaaaaact
    50881 ctggcgcaag gatcagggcc agaaaacctg cagccagcgt caccccagta tcgtagtgac
    50941 caaaatccag ctcgccgaga taggaaaaac caaagtagac cgccaccaga gcaattgaca
    51001 gcgaggtaaa aaattcgaga atgccggagg ataaaaacgc cagtcgtagc acttccattg
    51061 tccgttggcg gaaatcttcc gaagcagaac gaatactttc aatttcagct tcaccacgac
    51121 caaaaatacg caatgtttcc atgccgcgca ggcgatcgag gaaatgccca cttaagcgag
    51181 caagagcgag aaagttacgc cggttagcat cggcagcccc cattccaacc agcgccataa
    51241 acaacggaat tagcggtgct gtgcccagca gaatgagcgc cgcagcccag ttagagggga
    51301 agatcgccac cacaatcagc aacggcaccg acactgccag cgccatttgc ggcagatagc
    51361 gtgcatagta atcatgcata tcgtcaattt gctcgagtac cagcgtcgcc cagctccccg
    51421 caggtttacc ctgaatccac gctggccctg cttgttgcag acggtcgaga acctgacggc
    51481 ggatggcaaa gcggatatgc tgcccggcgt gataacccac ccgttcgcgt aaccagacca
    51541 cccatgcgcg caatacaaag gtcagaacca gtaacgtaaa gggaagcagc agggcttcac
    51601 ggggaatatt ctccataatc atatgttgca gaattcgcgc catgaaccag gcctgggcaa
    51661 tgatcaatat gccgcttaca aagcccagca gacgagaaat attcagccaa cgttgggaga
    51721 tgacgctttg ctgttttaac cagcgggtta actctttttg acgagattta ttcattgcac
    51781 gcttagcagg tgagttatca gaattatttg cagagcaatg ttacaacggg gaaaaaataa
    51841 aggcgaccca tagtcgcaag gtgtcgcctt ctttactttt gttactgatt tgtaaaatta
    51901 ttttgcgtca gctaaaccat cgaggtagcg ttccgcatca agtgctgcca tgcagccagt
    51961 accggccgaa gtaatggcct ggcgataaat gtgatccatc acgtcgcctg cggcaaagac
    52021 gccaggaatg ctggtctggg tggcattacc atgaataccc gactgtactt tgatgtagcc
    52081 gttttccagt tccagctgcc cttcgaaaat cgcagtattc gggctgtgac cgatagcaac
    52141 aaacagaccg gcaacgtcga gtgactcgat gttatcgctg ttttgcgtat cgcgcagacg
    52201 aacgccagtg acacccattt gatcgccggt cacttcttcc agcgtacggt tggtgtgcag
    52261 aatgatgttg ccgttctcca ctttatccat cagacgctta ataaggattt tttccgcgcg
    52321 gaaaccgtca cggcggtgaa tcagatgcac ttccgaagcg atgttagaca gatacagcgc
    52381 ctcttcaacc gcggtattgc cgccgccgat gaccgcaact ttctggttgc gatagaagaa
    52441 accgtcgcag gttgcacaag cagaaacccc acggccttta aaggcttctt cagagggcag
    52501 gccgagatag cgtgcagaag ctccggtggc aataatcagc gcgtcgcaag tgtattcgcc
    52561 gttatcgcca ttcagacgga acggacggtt ttgcagatcc accttgttga tatgatcaaa
    52621 aatgatctca gtttcaaact tggtggcatg ttcgtgcatg cgcgccatta ataacggacc
    52681 ggtcagatcg tttggatcac caggccagtt ttccacttcc gtggtggtgg tcagttggcc
    52741 gcctttttcc atgccggtaa tcagcacagg ttgcaggttg gcgcgcgccg cgtagacagc
    52801 agcggtgtat cccgccgggc ctgaacccag gataagcagt ttactgtgtt tggtcgtgcc
    52861 catgagatcc ccatagttgt tggcagacaa tgggcaggat tgtagggaat ttacagacgt
    52921 aaaaaaagag tatgacgatt ttgttaacaa tttgtgcaat cggcagcatc gataagcagg
    52981 tcaaattctc ccgtcattat cacctctgct acttaaattt cccgctttat aagccgatta
    53041 aatgatgaat aaacgcccct gttaatgaat atctggcatg ttgtactaaa aatcgatgtt
    53101 ttgctttgac aatcccctgg tgttttgcga aaacattcga ggaagaaaaa aaacagtatt
    53161 cttatatgcg cataaccatg tatgtaaata ccatgtttac cgtgctagtg aaatctacgt
    53221 atggcgtgga cagacgccat tcgtgatgtc gatagctgcc acaaggcaac ggtcttctca
    53281 ccgtagaccc aggcattgcg cgccgtgaat cttcatgatt tcggtctatc gtgacgggta
    53341 gcgactctga acagtgatgt ttcagggtca gacaggagta gggaaggaat acagagagac
    53401 aataataatg gtagatagca agaagcgccc tggcaaagat ctcgaccgta tcgatcgtaa
    53461 cattcttaat gagttgcaaa aggatgggcg tatttctaac gtcgagcttt ctaaacgtgt
    53521 gggactttcc ccaacgccgt gccttgagcg tgtgcgtcgg ctggaaagac aagggtttat
    53581 tcagggctat acggcgctgc ttaaccccca ttatctggat gcatcacttc tggtattcgt
    53641 tgagattact ctgaatcgtg gcgcaccgga tgtgtttgaa caattcaata ccgctgtaca
    53701 aaaacttgaa gaaattcagg agtgtcattt agtatctggt gatttcgact acctgttgaa
    53761 aacacgcgtg ccggatatgt cagcttaccg taagttgctg ggggaaaccc tgctgcgtct
    53821 gcctggcgtc aatgacacac ggacatacgt tgttatggaa gaagtcaagc agagtaatcg
    53881 tctggttatt aagacgcgct aacacggaac aggtgcaaaa tcggcgtatt ttgattacac
    53941 tcctgttaat ccatacagca acagtactgg ggtaacctgg tactgttgtc cgttttagca
    54001 tcgggcagga aaagcctgta acctggagag cctttcttga gccaggaata cactgaagac
    54061 aaagaagtca cattgacaaa gttaagtagc ggccgccgcc ttctggaggc gttgctgatc
    54121 cttattgtcc tgtttgccgt ctggttgatg gctgccttac taagctttaa cccttcggac
    54181 cccagctggt cgcaaacggc ctggcatgaa cctatccata atttaggtgg gatgcccggt
    54241 gcgtggttgg cagatacgct gttctttatt tttggcgtga tggcttacac cattcccgtc
    54301 attattgtcg gcggttgttg gtttgcctgg cgtcatcagt ctagcgacga atacattgat
    54361 tattttgccg tttcgctacg catcattggc gttttggcgc tcatccttac ctcctgtggt
    54421 ctggcggcaa tcaacgctga cgatatctgg tattttgcct ccggtggcgt cattggcagc
    54481 ttactaagca ctacgctaca accactgcta cacagtagcg ggggaactat tgcgctgctc
    54541 tgcgtttggg cagcgggcct gacgttgttc accggttggt catgggtgac cattgctgaa
    54601 aaactcggcg gctggatttt aaacattctc accttcgcca gtaatcgtac ccgtcgcgat
    54661 gatacctggg tcgatgaaga tgagtatgaa gacgacgaag agtatgaaga tgaaaatcac
    54721 ggcaaacagc atgaatcacg ccgtgcccgt attcttcgcg gcgcgctagc gcgtcgtaaa
    54781 cggttggcgg aaaaattcat taatccgatg gggcggcaaa cagacgctgc gttgttctcc
    54841 ggtaagcgga tggatgatga cgaagagatt acctacactg cacgcggtgt ggctgtcgac
    54901 ccggacgacg tcctattttt gggcaatcgt gcaacgcagc cagaatatga cgaatacgat
    54961 ccattattaa acggtgcgcc aattaccgaa cctgtcgctg tagcagctgc tgctaccacg
    55021 gcgacacaaa gctgggctgc gccggttgaa cctgtgactc agacgccgcc tgttgcctct
    55081 gttgatgttc caccttcgca acctacagta gcctggcagc ctgtaccggg tccacaaacg
    55141 ggagagccgg ttattgctcc tgcaccggaa ggttacccac agcagtcaca atatgcgcag
    55201 cctgcagtgc aatataatga gccgctgcaa caaccagtac agccgcagca gccgtattat
    55261 gcacctgcag ctgaacaacc tgcgcaacaa ccttactacg ctcctgcggc ggaacaacct
    55321 gttcagcaac cgtattacgc aactgcgccg gaacagcctg cgcaacagcc gtattatgcc
    55381 cctgcgccag aacaaccggt ggcaggtaac gcctggcaag ccgaagagca gcaatccatt
    55441 tttgctccac agtctacata ccagactgag caaacttatc agcagccagc cgctcaggag
    55501 ccgttgtacc aacagccgca acccgttgaa cagcagcctg ttgtggagcc tgaacccgtt
    55561 gtagaagaga caaaacccgc gcgtccgccg ctttactatt ttgaagaagt ggaagagaag
    55621 cgagcccgtg aacgtgaaca acttgcggcc tggtatcaac cgattccaga accggttaaa
    55681 gaaccagaac cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt acctccagta
    55741 gaagccgctg ccgctgtttc tccgctggca tctggcgtga aaaaagcgac actggcgacg
    55801 ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt
    55861 ccgcaggtca aagaggggat tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca
    55921 actcgtcgtg aattggcgtc ttacggtatt aagctgccct cacagcgtgc ggcggaagaa
    55981 aaagcccgtg aagcccagcg caatcagtat gattctggcg atcagtataa cgatgatgaa
    56041 atcgatgcga tgcagcagga tgaactggca cgtcagttcg cccagacaca gcagcaacgc
    56101 tatggcgaac agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga tgctgcggca
    56161 gaggctgaac tggctcgtca gtttacgcaa actcaacaac aacgttattc cggcgaacaa
    56221 ccggctgggg cgaatccgtt ctcgctggat gattttgaat tttcgccaat gaaagcgttg
    56281 ctggatgatg gtccacacga accgctgttt acgccaattg ttgaacctgt acagcagccg
    56341 caacaaccgg ttgcaccaca gcagcagtat cagcagccac aacagccggt tgcgccacag
    56401 ccgcagtatc agcagccaca acagcaggtt gcgccgcagc cacaatatca gcagccgcaa
    56461 caaccggttg cgccacagca gcaatatcag cagccgcaac aaccagttgc gccgcagccg
    56521 cagtatcaac agccgcaaca accggttgca ccgcagccgc agtatcagca gccacaacag
    56581 ccagttgcgc cacagccgca ggataccctg cttcatccgc tgttgatgcg taatggcgac
    56641 agccgtccgt tgcacaaacc gacgacgccg ctgccttctc tggatttgct gacaccgccg
    56701 ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggcgcg tctggtggaa
    56761 gcgcgtctgg ctgatttccg tattaaagcc gatgtcgtta attactctcc ggggccagtt
    56821 atcactcgct ttgaacttaa cctggctcct ggcgtaaaag cggcgcgcat ttctaacttg
    56881 tcacgggacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga agttattcct
    56941 ggcaaaccct atgtaggtct ggagttacca aataaaaaac gacaaaccgt ttatctgcgc
    57001 gaagttttgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt ggtgctgggt
    57061 aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca cttgttggtt
    57121 gcggggacta ccggttccgg taaatctgtc ggtgtgaacg cgatgatcct gagcatgctt
    57181 tataaagcac agccagaaga tgtgcgtttc atcatgatcg acccgaaaat gctggagctt
    57241 tcggtttatg aaggcattcc gcacctgtta acggaagtcg ttactgatat gaaagatgcc
    57301 gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc ggtataaact gatgtctgcg
    57361 ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga tcgcatgatg
    57421 cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca gcatccggtg
    57481 ctgaaaaaag aaccatacat tgtcgtgttg gttgacgaat ttgccgacct gatgatgacg
    57541 gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg tgccgcgggt
    57601 atccacctcg tactggcaac tcagcgtcca tcggttgatg ttattactgg tctgattaaa
    57661 gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc acgtaccatt
    57721 cttgatcagg ctggcgcgga atcactgctg ggtatggggg atatgctcta ctctgggccg
    57781 aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga agttcatgcc
    57841 gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat cacctccgac
    57901 agcgaaagcg aaggtggtgc gggtggtttc gatggcgctg aagaactgga tccgttgttc
    57961 gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg cgtacagcgt
    58021 cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga agcgcagggg
    58081 attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggccccacc gccgtttgac
    58141 taattaatgc attgccggat aaggcgcggt agcgtcgcat ccggcactct atcaactgaa
    58201 aattcagtat tttcttcttt cctcaagctg attattagcc tggaatagag agtagaggga
    58261 actcccgatc gggagtgacg taatttgagg aataatgatg aaaaaaattg ctatcacctg
    58321 tgcattactc tcaagcttag tagcaagcag cgtttgggct gatgccgcaa gcgatctgaa
    58381 aagccgcctg gataaagtca gcagcttcca cgccagcttc acacaaaaag tgactgacgg
    58441 tagcggcgcg gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc caaacttatt
    58501 caactggcat atgacacaac ctgatgaaag cattctggtt tctgacggta aaacactgtg
    58561 gttctataac ccgttcgttg agcaagctac ggcaacctgg ctgaaagatg ccaccggtaa
    58621 tacgccgttt atgctgattg cccgcaacca gtccagcgac tggcagcagt acaatatcaa
    58681 acagaatggc gatgactttg tcctgacgcc gaaagccagc aatggcaatc tgaagcagtt
    58741 caccattaac gtgggacgtg atggcacaat ccatcagttt agcgcggtgg agcaggacga
    58801 gcagcgcagc agttatcagc tgaaatccca gcaaaatggg gctgtggatg cagcgaaatt
    58861 taccttcacc ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga ggcacctgag
    58921 tgagcaatct gtcgctcgat ttttcggata atacttttca acctctggcc gcgcgtatgc
    58981 ggccagaaaa tttagcacag tatatcggtc agcaacattt gctggctgcg gggaagccgt
    59041 tgccgcgcgc tatcgaagcc gggcatttgc attctatgat cctctggggg ccaccgggta
    59101 ccggcaaaac aaccctcgct gaagtgattg cccgctatgc gaacgctgat gtggaacgta
    59161 tttctgccgt cacctctggc gtgaaagaga ttcgcgaggc gatcgagcgc gcccggcaaa
    59221 accgcaatgc aggtcgccgc actattcttt ttgttgacga agttcaccgt ttcaacaaaa
    59281 gccagcagga tgcatttctg ccacatattg aagacggcac catcactttt attggcgcaa
    59341 ccactgaaaa cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc cgtgtttatc
    59401 tgttgaaatc cctgagtaca gaggatattg agcaagtact aactcaggcg atggaagaca
    59461 aaacccgtgg ctatggtggt caggatattg ttctgccaga tgatacacga cgtgccattg
    59521 cagaactggt gaatggcgac gcgcgccggg cgttaaatac gctggaaatg atggcggata
    59581 tggccgaagt ctatgatagc ggtaagcggg tcctgaagcc tgaattactg accgaaatcg
    59641 ccggtgaacg tagcgcccgc tttgataaca aaggcgatcg cttttacgat ctaatttccg
    59701 cactgcataa gtcggtacgt ggtagcgcac ccgatgcggc gctgtactgg tatgcgcgaa
    59761 ttattaccgc tggtggcgat ccgttatatg tcgcgcgtcg ctgtctggcg attgcgtctg
    59821 aagacgtcgg taatgccgat ccacgggcga tgcaggtggc aattgcggcc tgggattgct
    59881 ttactcgcgt tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt gtttacctgg
    59941 cctgcgcgcc aaaaagcaac gctgtctaca ctgcgtttaa agccgcgctg gccgatgctc
    60001 gcgaacgccc ggattatgac gtgccggttc atttgcgtaa tgcgccgacg aaattaatga
    60061 aggaaatggg ctacgggcag gaatatcgtt acgctcatga tgaagcaaac gcttatgctg
    60121 ccggtgaggt ttacttcccg ccggaaatag cacaaacacg ctattatttc ccgacaaaca
    60181 ggggccttga aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag gatcaaaata
    60241 gccccataaa acgctaccgt taatgttatc gttgcggtaa tgttgttact gtatccctgt
    60301 ggtcgcaggc tgtggccaca tctcccattt aattcgataa gcacaggata agcatgctcg
    60361 atcccaatct gctgcgtaat gagccagacg cagtcgctga aaaactggca cgccggggct
    60421 ttaagctgga tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta ttgcaggtca
    60481 aaacggaaaa cctgcaagcg gagcgtaact cccgatcgaa atccattggc caggcgaaag
    60541 cgcgcgggga agatatcgag cctttacgtc tggaagtgaa caaactgggc gaagagctgg
    60601 atgcagcaaa agccgagcta gatgctttac aggctgaaat tcgcgatatc gcgctgacca
    60661 tccctaacct gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac aacgttgaag
    60721 tcagccgctg gggtaccccg cgtgagtttg actttgaagt tcgtgaccac gtgacgctgg
    60781 gtgaaatgca ctctggcctc gactttgcag ctgcagttaa gctgactggt tcccgctttg
    60841 tggtaatgaa agggcagatt gctcgcatgc accgcgcact gtcgcagttt atgctggatc
    60901 tgcataccga acagcatggc tacagtgaga actatgttcc gtacctggtt aaccaggaca
    60961 cgctgtacgg tacgggtcag ctgccgaaat ttgctggcga tctgttccat actcgtccgc
    61021 tggaagaaga agcagacacc agtaactatg cgctgatccc aacggcagaa gttccgctga
    61081 ctaacctggt gcgcggtgaa atcatcgatg aagatgatct gccaattaag atgaccgccc
    61141 acaccccatg cttccgttct gaagccggtt catatggtcg tgacacccgt ggtctgatcc
    61201 gtatgcacca gttcgacaaa gttgaaatgg tgcagatcgt gcgcccagaa gactcaatgg
    61261 cggcgctgga agagatgact ggtcatgcag aaaaagtcct gcagttgctg ggcctgccgt
    61321 accgtaaaat catcctttgc actggcgaca tgggctttgg cgcttgcaaa acttacgacc
    61381 tggaagtatg gatcccggca cagaacacct accgtgagat ctcttcctgc tccaacgttt
    61441 gggatttcca ggcacgtcgt atgcaggcac gttgccgcag caagtcggac aagaaaaccc
    61501 gtctggttca taccctgaac ggttctggtc tggctgttgg tcgtacgctg gttgcagtaa
    61561 tggaaaacta tcagcaggct gatggtcgta ttgaagtacc agaagttctg cgtccgtata
    61621 tgaacggact ggaatatatt ggctaatacc caatttttct gaatctaaaa agcgccttta
    61681 gggcgctttt tttgtctccc tttgataccg aacaataatt actcctcact tacacgtaat
    61741 actactttcg agtgaaaatc tacctatctc tttgattttc aaattattcg atgtatacaa
    61801 gcctatatag cgagctgcta tagaaataat tacacaatac ggtttgttac tggaatcaat
    61861 cgtgatcaag cttgagtgag ccattatgaa aacgaaaatc cctgatgcgg tattggctgc
    61921 tgaggagagt cgccgtggtt tggtaaaaac gacagcgatc ggcggcctgg caatggccag
    61981 cagcgcatta acattacctt ttagtcggat tgcgcacgct gtcgatagcg ccattccaac
    62041 aaaatcagac gaaaaggtta tctggagcgc ctgtacagtt aactgtggta gtcgctgccc
    62101 gctacgtatg cacgtcgtgg acggtgaaat caaatatgtc gaaacggaca ataccggcga
    62161 tgacaattac gacggcctgc actaggttcg cgcctgcctg cgtgggcgtt ccatgcgtcg
    62221 ccgtgtctac aatccggacc gcctgaaata tccgatgaaa cgagtcgggg cgcgcggtga
    62281 aggcaaattc gagcgcatta gctgggaaga agcctacgac atcatcgcga ccaatatgca
    62341 gcgcctgatc aaagagtacg gcaacgagtc tatctatctg aactatggca ccggtacgct
    62401 gggcggcacc atgacccgct cctggccgcc gggaaatacc ctggtcgcgc gcctgatgaa
    62461 ctgctgcggc ggttatctta accattacgg tgactactct tccgcgcaaa ttgcggaagg
    62521 tttgaaccat acctacggcg gctgggcaga tggtaacagc ccgtcggata tcgaaaacag
    62581 taaattggta gtgctgtttg gtaataaccc aggcgaaacg cgaatgagtg gcggtggggt
    62641 gacttactat cttgaacagg cacgccagaa atctaatgct cgcataatca tcatcgatcc
    62701 gcgctatacc gacaccggtg ccgggcgcga agatgagtgg atccctattc gtccaggaac
    62761 agatgccgca ctggttaacg gtctggcgta cgtcatgatc actgaaaacc tggtggatca
    62821 ggcattcctc gataaatatt gcgttggcta tgacgagaaa accctgcccg ccagtgcgcc
    62881 gaaaaatggt cattataaag cttatattct gggtgaaggg ccagatggcg tggctaaaac
    62941 accggaatag gcctcgcaaa tcaccggtgt tccggcagac aaaatcatca aactggctcg
    63001 tgaaatcggc agtaccaaac cggcgtttat cagccaggga tggggcccgc agcgtcacgc
    63061 taacggtgaa atcgcaaccc gtgctatctc gatgctggcg attctgaccg gtaacgttgg
    63121 tattaatgga gggaacagcg gcgcgcgtga aggttcatac agcttaccgt ttgtccgtat
    63181 gccgaccttg gaaaacccga tccagaccag catttcgatg tttatgtgga ccgatgccat
    63241 tgaacgtggc ccggaaatga cggcgctgcg tgatggtgtg cgcgggaaag ataagctgga
    63301 tgtgccgatc aaaatgatct ggaactatgc cggtaactgc ctgattaacc agcattctga
    63361 aatcaaccgt acccatgaaa tcctccagga tgataagaag tgcgagctga ttgtggttat
    63421 cgactgccac atgacctcat cggcgaaata tgctgacatc ctgctgcctg actgcaccgc
    63481 ttccgaacag atggactttg cactggatgc atcctgcggg aatatgtctt acgtgatttt
    63541 caacgatcag gtgattaaac cgcgctttga atgtaagacc atctatgaaa tgaccagcga
    63601 actggcaaaa cgtcttggcg ttgagcaaca gtttactgaa ggccgtaccc aggaagagtg
    63661 gatgcggcat ctgtatgccc agtcgcggga agcgattcct gaactgccaa cgtttgaaga
    63721 gttccgcaag caggggatct ttaaaaagcg cgacccacaa gggcatcacg ttgcttataa
    63781 agccttccgt gaagatccgc aggcaaaccc actgactacg ccatcgggca aaattgagat
    63841 ttattcgcag gcgctggctg acattgccgc tacctgggaa ttgccagaag gcgatgtgat
    63901 cgatccactg ccgatctaca cgccgggctt tgaaagttat caggatccgc tgaacaaaca
    63961 gtatccgctg cagcttacag gtttccacta taaatctcgc gttcactcaa cttacggcaa
    64021 cgttgatgtg ctgaaagcgg cttgccgtca ggaaatgtgg atcaacccgc ttgatgccca
    64081 aaaacgcggt atccacaacg gcgataaagt caggatcttt aacgatcgtg gtgaggttca
    64141 tattgaggcg aaagtgacgc cacgaatgat gccgggcgtg gtcgcactgg gtgaaggcgc
    64201 ctggtatgac ccggatgcaa aacgtgtcga taagggcggt tgtatcaacg tactgaccac
    64261 tcaacgtccg tctcctctcg ctaaggggaa tccgtcacat acaaaccttg ttcaggttga
    64321 aaaggtgtaa ggagtaaccg atgacaaccc agtatggatt ttttattgat tccagccgtt
    64381 gcaccggttg caaaacctgc gagctggctt gtaaagacta caaagatttg acgccagaag
    64441 tcagtttccg ccgcatttat gaatatgctg gcggcgactg gcaggaagat aacggtgtct
    64501 ggcaccagaa cgtgtttgcc tactatctgt cgatttcgtg caaccactgc gaagatccgg
    64561 cttgtactaa agtctgcccg agcggtgcga tgcataaacg cgaagatggt tttgttgtgg
    64621 tcgatgaaga tgtatgcatt ggctgccgtt actgtcatat ggcttgcccg tatggcgcac
    64681 cgcaatataa cgaaacgaaa ggccatatga ccaaatgcga tggttgttat gaccgtgttg
    64741 cggagggtaa aaagccgatc tgtgttgaat cttgcccgct gcgggcgctg gatttcggcc
    64801 ctatcgacga gctgcgtaaa aaacatggcg atctggcggc cgttgcgccg ttgccgcgag
    64861 ctcactttac caaaccgaat attgtgatca aacccaatgc caatagccgc ccgacagggg
    64921 ataccactgg ctatctggca aacccgaagg aggtgtgaga tgggaagtgg atggcatgaa
    64981 tggccgctga tgatcttcac ggtcttcggg caatgtgtgg cgggtggttt tatcgttctg
    65041 gctttggcgc tgctcaaagg cgacctgcga gcagaagccc agcagcgtgt tatcgcctgc
    65101 atgtttggct tatgggtact gatgggcatt ggctttatcg cctctatgct ccatcttggt
    65161 tcaccgatgc gcgcctttaa ctcgctcaac cgggtagggg cttcagcact cagtaacgaa
    65221 atcgccagcg gttcgatctt ttttgctgta ggcggcatcg gctggttgct ggcaatgctg
    65281 aaaaagctgt caccggcatt gcgtacgcta tggctggtgg tgacgatggt tcttggcgtc
    65341 gtctttgtct ggatgatggt gcgtgtgtat aacagcattg ataccgttcc gacctggtac
    65401 agcatctgga cgccgatggg cttcttcctg acgatgttca tgggcggccc gttactgggt
    65461 tacctgttgc tgagtctggc tggcgtcgat ggctgggcga tgcgtctgct gccagccatt
    65521 tctgtactgg cactggtggt gagtggcgtg atgtcagtga tgcagggcac agagctggca
    65581 actattcata gttctgtgca gcaggccgca gcgctggtgc cggactatgg tgcgctgatg
    65641 tcctggcgga tcgtgctttt ggccgttgcc ctgtgcttgt ggattgcacc acagctaaaa
    65701 ggttatcagc ctgcggttcc gctactatcc gtctcattca ttctgcttct ggcaggggaa
    65761 ttaatcggtc gcggcgtatt ctatggtttg catatgaccg tggggatggc cgtcgcaagc
    65821 taatcagtaa caaccggggt ttcggccccg gttctctttt atttctgctt cgttaacgtg
    65881 tcataactgg tcatcaaatt acgataatca ggaatatggt tagagaacaa cgtcgccagt
    65941 ccttcaatat cattacgcca gtcacggtgc agctcgcaag ccacgccgaa ccatgtcatc
    66001 aattgcgcac cagcttgcga catgcgatcc cacgccgaat gccgggtaat ttcattaaag
    66061 gtccctgatg catcagtcac gacaaagaca tcaaaccctt cttcaatggc tgaaagcgcc
    66121 gggaatgcca cgcaaacttc ggttaccaca ccggcaataa ttaactgttt tttacctgtc
    66181 gctttgactg cttttacaaa atcttcgtta tcccaggcgt taatattgcc cgggcgagca
    66241 atgtaaggtg catcggggaa ttgtgctttc agttctagaa ctaatgggcc gttaggaccg
    66301 gtttcaaaac tggtggtaag aatagttggt aagttgaaat acttagccag gtcacccagc
    66361 gccagcacgt tatttttaaa cttatcgggt tcgatatccc gtacaaggga aagtaaacca
    66421 gcctggtgat caacaagcaa aacagcagca tcatttttat caagacgaac atacggtttg
    66481 gtcatcctct tctcctttcg agagtcagtg gtacgtctga aatcggtcag acgcgatgca
    66541 ttgctctgaa agcatagacg ggaaatatga gtttgctgtg accatgaaat ttttcgactg
    66601 aacgaagcgt atgaaatttg tgttagatca ataaaaacaa tcagatgaga ttaatcatct
    66661 cttcagcatt agcgttatga atgagtaata taaatcgata ataccgccgc tgcgatatgc
    66721 gcgtattttc acccattgac aatgtttttg gcggtggcat gatgcgcatg aaatttgaac
    66781 ttcctcacgg ttttaattca tgtccacgta tacccggcct gtcatgcttt tgctgtctgg
    66841 cctgcttttg ttgactctgg cgatcgcggt gttaaataca ctcgtgccgc tttggctcgc
    66901 ccaggaacac atgtccacat ggcaggtagg cgttgtcagc tcatcctatt ttaccggcaa
    66961 ccttgtcggt acattgctga cagggtatgt cattaaacgc attggcttta accgcagcta
    67021 ttatctgtcc tccttcattt ttgctgctgg ctgtgccggc cttggcctga tgattggttt
    67081 ctggagctgg ttggcgtggc gttttgtcgc gggcgtcggc tgtgccatga tttgggtggt
    67141 tgttgagagc gcgctgatgt gcagtgggac gtcacgtaac cgtgggcgtt tgcttgctgc
    67201 gtatatgatg gtttattacg tgggaacgtt tttaggccag ttactggtca gcaaagtctc
    67261 aaccgagctg atgagcgtat tgccgtgggt tacaggtttg acattggcag ggatcttacc
    67321 gctgttgttt acgcgtgtcc tgaatcagca ggctgaaaac catgattcga cgtcaattac
    67381 ggcaatgcta aaactccgtc aggcgcggct tggcgtgaat ggctgcatta tctcaggaag
    67441 cgttctggga tctctatatg gcctgatgcc tctgtacctc aatcacaaag gggtgagcaa
    67501 tgccagtatt ggtttctgga tggcggtact ggtcagtgcg ggtatcctcg gacaatggcc
    67561 gattggacgt ctggcggata agtttggtcg actgctggtt ttgcgtgttc aggtctttgt
    67621 cgtcattctc ggcagtattg cgatgcttag ccaggcggcg atggccccag cgttattcat
    67681 cctcggtgcc gctggcttta cg
//
LOCUS       DAJTYG010000007        67082 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTYG010000007 DAJTYG010000000
VERSION     DAJTYG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.007"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(64..1068)
                     /gene="cra"
                     /locus_tag="OO568_000516"
     CDS             complement(64..1068)
                     /gene="cra"
                     /locus_tag="OO568_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6640004.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1150..1236)
                     /locus_tag="OO568_000517"
     CDS             complement(1150..1236)
                     /locus_tag="OO568_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6640005.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="OO568_000518"
     CDS             complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="OO568_000518"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6640006.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1742..3459)
                     /gene="ilvI"
                     /locus_tag="OO568_000519"
                     /pseudo
     CDS             complement(1742..3459)
                     /gene="ilvI"
                     /locus_tag="OO568_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
     gene            3578..3622
                     /locus_tag="OO568_000520"
     CDS             3578..3622
                     /locus_tag="OO568_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640007.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3777..4721)
                     /gene="leuO"
                     /locus_tag="OO568_000521"
     CDS             complement(3777..4721)
                     /gene="leuO"
                     /locus_tag="OO568_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6640008.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5382..5468
                     /gene="leuL"
                     /locus_tag="OO568_000522"
     CDS             5382..5468
                     /gene="leuL"
                     /locus_tag="OO568_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6640009.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5561..7132
                     /gene="leuA"
                     /locus_tag="OO568_000523"
     CDS             5561..7132
                     /gene="leuA"
                     /locus_tag="OO568_000523"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6640010.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPGHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7132..8223
                     /gene="leuB"
                     /locus_tag="OO568_000524"
     CDS             7132..8223
                     /gene="leuB"
                     /locus_tag="OO568_000524"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6640011.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNKIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8226..9626
                     /gene="leuC"
                     /locus_tag="OO568_000525"
     CDS             8226..9626
                     /gene="leuC"
                     /locus_tag="OO568_000525"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6640012.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKSGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9637..10242
                     /gene="leuD"
                     /locus_tag="OO568_000526"
     CDS             9637..10242
                     /gene="leuD"
                     /locus_tag="OO568_000526"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6640013.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10291..11469)
                     /gene="setA"
                     /locus_tag="OO568_000527"
     CDS             complement(10291..11469)
                     /gene="setA"
                     /locus_tag="OO568_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6640014.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11571..11701)
                     /gene="sgrT"
                     /locus_tag="OO568_000528"
                     /pseudo
     CDS             complement(11571..11701)
                     /gene="sgrT"
                     /locus_tag="OO568_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
     gene            11790..13445
                     /gene="sgrR"
                     /locus_tag="OO568_000529"
     CDS             11790..13445
                     /gene="sgrR"
                     /locus_tag="OO568_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6640015.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGDLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13479..13578
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13609..14592
                     /gene="thiB"
                     /locus_tag="OO568_000530"
     CDS             13609..14592
                     /gene="thiB"
                     /locus_tag="OO568_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6640016.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14568..16178
                     /gene="thiP"
                     /locus_tag="OO568_000531"
     CDS             14568..16178
                     /gene="thiP"
                     /locus_tag="OO568_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6640017.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16162..16860
                     /gene="thiQ"
                     /locus_tag="OO568_000532"
     CDS             16162..16860
                     /gene="thiQ"
                     /locus_tag="OO568_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6640018.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIVWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16974..17738)
                     /locus_tag="OO568_000533"
     CDS             complement(16974..17738)
                     /locus_tag="OO568_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6640019.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17824..18702)
                     /gene="araC"
                     /locus_tag="OO568_000534"
     CDS             complement(17824..18702)
                     /gene="araC"
                     /locus_tag="OO568_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6640020.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19041..20740
                     /gene="araB"
                     /locus_tag="OO568_000535"
                     /pseudo
     CDS             19041..20740
                     /gene="araB"
                     /locus_tag="OO568_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            20751..22253
                     /gene="araA"
                     /locus_tag="OO568_000536"
                     /pseudo
     CDS             20751..22253
                     /gene="araA"
                     /locus_tag="OO568_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            22453..23148
                     /gene="araD"
                     /locus_tag="OO568_000537"
     CDS             22453..23148
                     /gene="araD"
                     /locus_tag="OO568_000537"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6640021.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSDVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23223..25574
                     /gene="polB"
                     /locus_tag="OO568_000538"
     CDS             23223..25574
                     /gene="polB"
                     /locus_tag="OO568_000538"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6640022.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWVRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25739..28645
                     /gene="rapA"
                     /locus_tag="OO568_000539"
     CDS             25739..28645
                     /gene="rapA"
                     /locus_tag="OO568_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6640023.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNTVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28657..29316
                     /gene="rluA"
                     /locus_tag="OO568_000540"
     CDS             28657..29316
                     /gene="rluA"
                     /locus_tag="OO568_000540"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6640024.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARAVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29433..30248)
                     /gene="djlA"
                     /locus_tag="OO568_000541"
     CDS             complement(29433..30248)
                     /gene="djlA"
                     /locus_tag="OO568_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6640025.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30503..32857
                     /gene="lptD"
                     /locus_tag="OO568_000542"
     CDS             30503..32857
                     /gene="lptD"
                     /locus_tag="OO568_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6640026.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32910..34196
                     /gene="surA"
                     /locus_tag="OO568_000543"
     CDS             32910..34196
                     /gene="surA"
                     /locus_tag="OO568_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6640027.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKLSEEAASWMQEQRASAYVKILSN"
     gene            34196..35185
                     /gene="pdxA"
                     /locus_tag="OO568_000544"
     CDS             34196..35185
                     /gene="pdxA"
                     /locus_tag="OO568_000544"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6640028.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGITEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35182..36003
                     /gene="rsmA"
                     /locus_tag="OO568_000545"
     CDS             35182..36003
                     /gene="rsmA"
                     /locus_tag="OO568_000545"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6640029.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36006..36383
                     /gene="apaG"
                     /locus_tag="OO568_000546"
     CDS             36006..36383
                     /gene="apaG"
                     /locus_tag="OO568_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6640030.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36390..37232
                     /gene="apaH"
                     /locus_tag="OO568_000547"
     CDS             36390..37232
                     /gene="apaH"
                     /locus_tag="OO568_000547"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6640031.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGMPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37310..37789)
                     /gene="folA"
                     /locus_tag="OO568_000548"
     CDS             complement(37310..37789)
                     /gene="folA"
                     /locus_tag="OO568_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6640032.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37980..39842)
                     /gene="kefC"
                     /locus_tag="OO568_000549"
     CDS             complement(37980..39842)
                     /gene="kefC"
                     /locus_tag="OO568_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6640033.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39835..40365)
                     /gene="kefF"
                     /locus_tag="OO568_000550"
     CDS             complement(39835..40365)
                     /gene="kefF"
                     /locus_tag="OO568_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6640034.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40413..41807)
                     /locus_tag="OO568_000551"
     CDS             complement(40413..41807)
                     /locus_tag="OO568_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6640035.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41866..42153)
                     /gene="fixX"
                     /locus_tag="OO568_000552"
     CDS             complement(41866..42153)
                     /gene="fixX"
                     /locus_tag="OO568_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6640036.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42150..43436)
                     /locus_tag="OO568_000553"
     CDS             complement(42150..43436)
                     /locus_tag="OO568_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6640037.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADFAPVERLITHEKLAFMTEKSAMTMDYCNGDETSLSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIESPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESSPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43487..44428)
                     /locus_tag="OO568_000554"
     CDS             complement(43487..44428)
                     /locus_tag="OO568_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6640038.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQTRQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVRISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(44443..45213)
                     /locus_tag="OO568_000555"
     CDS             complement(44443..45213)
                     /locus_tag="OO568_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6640039.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45684..47198
                     /gene="caiT"
                     /locus_tag="OO568_000556"
     CDS             45684..47198
                     /gene="caiT"
                     /locus_tag="OO568_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6640040.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47229..48371
                     /gene="caiA"
                     /locus_tag="OO568_000557"
     CDS             47229..48371
                     /gene="caiA"
                     /locus_tag="OO568_000557"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6640041.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48500..49717
                     /gene="caiB"
                     /locus_tag="OO568_000558"
     CDS             48500..49717
                     /gene="caiB"
                     /locus_tag="OO568_000558"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR6640042.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49791..51344
                     /gene="caiC"
                     /locus_tag="OO568_000559"
     CDS             49791..51344
                     /gene="caiC"
                     /locus_tag="OO568_000559"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6640043.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51453..52238
                     /gene="caiD"
                     /locus_tag="OO568_000560"
     CDS             51453..52238
                     /gene="caiD"
                     /locus_tag="OO568_000560"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6640044.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52244..52834
                     /gene="caiE"
                     /locus_tag="OO568_000561"
     CDS             52244..52834
                     /gene="caiE"
                     /locus_tag="OO568_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6640045.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52920..53315)
                     /gene="caiF"
                     /locus_tag="OO568_000562"
     CDS             complement(52920..53315)
                     /gene="caiF"
                     /locus_tag="OO568_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6640046.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53577..56798)
                     /gene="carB"
                     /locus_tag="OO568_000563"
     CDS             complement(53577..56798)
                     /gene="carB"
                     /locus_tag="OO568_000563"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6640047.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56816..57964)
                     /gene="carA"
                     /locus_tag="OO568_000564"
     CDS             complement(56816..57964)
                     /gene="carA"
                     /locus_tag="OO568_000564"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6640048.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58420..59241)
                     /gene="dapB"
                     /locus_tag="OO568_000565"
     CDS             complement(58420..59241)
                     /gene="dapB"
                     /locus_tag="OO568_000565"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6640049.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59408..60322)
                     /gene="rihC"
                     /locus_tag="OO568_000566"
     CDS             complement(59408..60322)
                     /gene="rihC"
                     /locus_tag="OO568_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6640050.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60388..61338)
                     /gene="ispH"
                     /locus_tag="OO568_000567"
                     /gene_synonym="lytB"
     CDS             complement(60388..61338)
                     /gene="ispH"
                     /locus_tag="OO568_000567"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6640051.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61340..61789)
                     /gene="fkpB"
                     /locus_tag="OO568_000568"
     CDS             complement(61340..61789)
                     /gene="fkpB"
                     /locus_tag="OO568_000568"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6640052.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61876..62370)
                     /gene="lspA"
                     /locus_tag="OO568_000569"
     CDS             complement(61876..62370)
                     /gene="lspA"
                     /locus_tag="OO568_000569"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6640053.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62370..65186)
                     /gene="ileS"
                     /locus_tag="OO568_000570"
     CDS             complement(62370..65186)
                     /gene="ileS"
                     /locus_tag="OO568_000570"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6640054.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAAILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65229..66170)
                     /gene="ribF"
                     /locus_tag="OO568_000571"
     CDS             complement(65229..66170)
                     /gene="ribF"
                     /locus_tag="OO568_000571"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6640055.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66178..66396)
                     /locus_tag="OO568_000572"
     CDS             complement(66178..66396)
                     /locus_tag="OO568_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6640056.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66499..66762
                     /gene="rpsT"
                     /locus_tag="OO568_000573"
     CDS             66499..66762
                     /gene="rpsT"
                     /locus_tag="OO568_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6640057.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16279 a        18249 c        17268 g        15286 t
ORIGIN      
        1 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
       61 ggcttagcta cggctgagca cgccgcggcg atagagatta cgtttaatgc gcgttaaacc
      121 aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc
      181 gacatcgcgg tgacgttgag ccactgccag caccggacac tgtaagaagt cgagcagttc
      241 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttgccgtcgc gacgcaacgt
      301 gacatccatc actccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg
      361 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
      421 atacaggaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      481 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      541 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      601 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      661 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      721 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      781 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      841 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      901 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      961 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
     1021 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
     1081 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
     1141 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
     1201 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
     1261 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
     1321 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1381 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1441 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1501 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1561 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1621 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1681 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1741 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1801 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1861 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1921 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1981 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     2041 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     2101 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     2161 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     2221 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     2281 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     2341 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2401 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2461 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2521 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2581 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2641 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2701 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
     2761 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2821 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2881 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2941 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     3001 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     3061 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     3121 actcctgaaa agcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     3181 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
     3241 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     3301 ggcctgctca tgacgaacta atacatgatc aataccaccc acggtatgca atgcatcgta
     3361 aatatcaagg actgcgcctc cgggataacc gaatacttgt ttaacgccct gatcgataag
     3421 cgatcggacg accatctcgg ctccagacaa catctccatg gcctgcctca ctgtttgacg
     3481 gaaaaaatgt gtaaaacaca ttaaccgtcc gatcttgctt aagcaattgg cagccagaga
     3541 cataaatgca gggtaataag aataaacagg aggaaaggtg aaaagaaacg ataaaatcct
     3601 ccattacgaa ggattaaatt gataataaat cggctgaatc ccacaactta cagcaatttc
     3661 tctatttttt taattaatcc taaaatagag agtccacaca ttcagaataa aattctacat
     3721 tcaataaaaa atgcagaata aaccagacat tcatgtctgg cctattctgc attcagttag
     3781 cgtttgcaaa ttgagactaa ttgttcttcc atccactgat ggcctttatc gcgcccggca
     3841 gcttcatgcc aagagagata acaggttctg ctgttttgtt ttaacggcag cggtaatacc
     3901 tgtaattcta aggattcagc gaactcttca gccagccaac gcggcgcaat agcgaccaaa
     3961 tgcgtttgcg acaccacgct aagtacgctc atcattgcca tgccctgata cgcgatactg
     4021 gcttgcttat ctaccgtgtc ataccaaggt tgactaaatg acgcgaaacg atcgagcgaa
     4081 accgccgcat gttgttcgtt ataaacatca tgtttcagta acgggccttt aattgttgga
     4141 tgatttttgc tggctaccag caccatttca tctttaaata atggcacgct ggtaaattca
     4201 ggacgatgga agtcttcata actaatcaca aactccgttt cctgataacg cagctgatgt
     4261 tcagtgttct gatttaatga agacttgaac ataacatgta tatttggcgc aatctgctca
     4321 atgtgattat aaatctgcga ggtcagaatg ctgtctaacg ggctgcaaac acaaagatga
     4381 aatacacgtt cactgctcgc gggttcaaaa cctgaaccag gcaattcatt ttgtactagt
     4441 tgcaatgcct ggcgaactga accaaaaagt tgaaatgcgc gagcagtcgg ttgaatacca
     4501 cggccataac gaacaaaaag ctcgtcatta aacatcacct tcaggcgtgc aacagcgtta
     4561 ctgaccgcag gttgcgacat acccagaaca tgagcggcac gggtaatgtt ttgctcctgc
     4621 atcacggcat cgaaaacggt taataagttg agatcgacca tgcgtagctg tggcttgctt
     4681 aactccgccg tctctgaatg atctgtttgt acctctggca tacttaactc cactgtcaca
     4741 cttaactccc tttcccttat tggaatgcag agaaataatc ctgaagaata tgatttatca
     4801 tgcatataaa ataagaaaaa gcaaaatgag taaaattcgg aaaacataaa gatacgggtt
     4861 ctgataaaaa cagaatccat tgccgtggaa tgagtcattt accacagcat aataatccat
     4921 aatgtttttg ttgcgaaaac aatctaatca taactaccgc gaatactcaa tcatctacaa
     4981 aatggattaa atgtgcttat ttaataatta ttttatatat ttaatgcatt aattcttaac
     5041 attaattgat caataatatt caccaaatca atatcaaaaa aaaatcgcaa aacatataat
     5101 tcaatacaaa tcatcaggat aggttttgca acgcgtgcat tttgtcccct ttttcctcgt
     5161 tgattagatg caaaaattta tgctgaaata tgtcaaccga tgaaaagcgt cggtagttaa
     5221 gcagaaatta atatcgctta ctttaaccac cgtagcacaa ttagctaatt ttacggatgc
     5281 agaactcacg ctggcgggac gtttttattg cgtcagggtt gacatccgtt tttgtatcca
     5341 gtaactctaa aagcatatcg cattcatctg gagctgattt aatgactcac atcgttcgct
     5401 ttatcggtct actactacta aacgcatctt ctttgcgcgg tagacgagtg agcggcatcc
     5461 agcattaagc cagcacgcag tcaaacaaaa aacccgcgcc attgcgcggg tttttttatg
     5521 cccgaagcga ggcgctctaa aagagacaag gacccaaacc atgagccagc aagtcattat
     5581 tttcgatacc acattgcgcg acggtgaaca ggcgttacag gcaagcttga gtgtgaaaga
     5641 aaaactgcaa attgcgctgg cccttgagcg tatgggtgtt gacgtgatgg aagtcggttt
     5701 ccccgtctct tcgccgggcg attttgaatc ggtgcaaacc atcgcccgcc aggttaaaaa
     5761 cagccgcgta tgtgcgttag ctcgctgcgt ggaaaaagat atcgacgtgg cggccgaatc
     5821 cctgaaagtc gccgaagcct tccgtattca tacctttatt gctacctccc caatgcacat
     5881 cgccaccaag ctgcgcagca ccctggacga ggtgatcgaa cgcgctatct atatggtgaa
     5941 acgcgcccgt aattacaccg atgatgttga attttcttgc gaagatgccg ggcgtacacc
     6001 cattgccgat ctggcgcgag tggtcgaagc ggcgattaat gccggtgcca ccaccatcaa
     6061 cattccggac accgtgggct acaccatgcc gtttgagttc gccggaatca tcagcggcct
     6121 gtatgaacgc gtacctaaca tcgacaaagc cattatttcc gtacataccc acgacgattt
     6181 aggcctggcg gtcggcaact cactggcggc ggtacatgcc ggtgcacgcc aggtggaagg
     6241 tgcaatgaac gggatcggcg agcgtgccgg taactgttcg ctggaagaag tcatcatggc
     6301 gattaaagtt cgtaaggata ttctcaacgt ccacaccgcc attaatcacc aggagatatg
     6361 gcgcaccagc cagttagtta gccagatttg taatatgccg atcccggcaa acaaagccat
     6421 tgttggcagc ggcgcattcg ctcactcctc cggtatccac caggatggcg tgctgaaaaa
     6481 ccgcgaaaac tacgaaatca tgacaccaga atctattggt ctgaaccaaa tccagctgaa
     6541 tctgacctct cgttcggggc gtgcggcggt gaaacatcgc atggatgaga tggggtataa
     6601 agaaagtgaa tataatttag acaatttgta cgatgctttc ctgaagctgg cggacaaaaa
     6661 aggtcaggtg tttgattacg atctggaggc gctggccttc atcggtaagc agcaagaaga
     6721 gccggggcat ttccgtctgg attacttcag cgtgcagtct ggctctaacg atatcgccac
     6781 cgccgccgtc aaactggcct gtggcgaaga agtcaaagca gaagccgcca acggtaacgg
     6841 tccggtcgat gccgtctatc aggcaattaa ccgcatcact gactataacg tcgaactggt
     6901 gaaatacagc ctgaccgcca aaggtcacgg taaagatgcg ctgggtcagg tggatattgt
     6961 cgccaactac aacggtcgcc gcttccacgg cgtcggcctg gccaccgata ttgtcgagtc
     7021 ctccgccaaa gccatggtgc acgtactgaa caatatctgg cgtgccgcag aagtcgaaaa
     7081 agagttgcaa cgcaaagctc aacacaacga aaacaacaag gaaaccgtgt gatgtcgaag
     7141 aattaccata ttgccgtatt gccgggagac ggtattggtc cggaagtgat gacccaggcg
     7201 ctgaaagtgc tgaatgccgt gcgcaaccgc tttgcgatgc gcatcaccac cagccattac
     7261 gatgtaggcg gcgcagccat tgataaccac gggcaaccac tgccgcctgc gacggttgaa
     7321 ggttgtgagc aagccgatgc cgtgctgttt ggctcggtag gcggcccgaa gtgggaacat
     7381 ttaccaccag accagcaacc agaacgcggc gcgctgctgc cgctgcgtaa gcacttcaaa
     7441 ttattcagca acctgcgccc ggcaaaactg tatcaggggc tggaagcatt ctgtccgctg
     7501 cgtgcagaca ttgccgctaa cggcttcgac atcctgtgcg tgcgcgaact gaccggcggc
     7561 atctatttcg gtcagccaaa aggccgcgaa ggtagcggac aatatgaaaa agcgtttgat
     7621 accgaggtgt atcaccgttt tgagatcgag cgtattgccc gcatcgcgtt tgaatctgct
     7681 cgtaaacgtc gccacaaagt cacctcaatc gacaaagcta acgtgctgca atcctctatt
     7741 ttatggcgtg aaatcgtcaa caagatcgcc acggaatacc cggatgtcga actggcgcat
     7801 atgtacatcg acaacgccac catgcagctg attaaagatc catcacagtt tgacgttctg
     7861 ctgtgctcca acctgtttgg cgacattctg tctgacgagt gcgcaatgat cactggctcg
     7921 atgggcatgt tgccttccgc cagcctgaac gagcaaggtt ttggactgta tgaaccggcg
     7981 ggcggctcgg caccagatat cgcaggcaaa aacatcgcca acccgattgc acaaatcctg
     8041 tcgctggcac tgctgctgcg ttacagcctg gatgccgatg atgcggcttc cgccattgaa
     8101 cgcgccatta accgtgcatt agaagaaggc attcgcaccg gggatttagc ccgtggcgct
     8161 gccgccgtta gtaccgatga aatgggcgat atcattgccc gctatgtggc agaaggggtg
     8221 taatcatggc taaaacgtta tacgaaaaat tattcgacgc tcacgtagtg tacgaagccg
     8281 aaaacgaaac cccgctgtta tatatcgacc gccacctggt gcatgaagtg acctcaccgc
     8341 aggcgttcga tggtctgcgc gcccacggtc gcccggtacg tcagccgggc aaaaccttcg
     8401 ccactatgga tcacaacgtc tctacccaga ccaaagacat taatgcctgt ggtgaaatgg
     8461 cgcgcatcca gatgcaggag ctgattaaaa actgcaaaga atttggcgtc gagctgtatg
     8521 acctgaatca cccgtatcag gggatcgtcc acgtaatggg gccggaacag ggcgtcacct
     8581 tgccggggat gaccattgtc tgcggcgact cgcataccgc cacccacggc gcgtttggcg
     8641 cactggcctt tggtatcggc acttccgaag ttgaacacgt actggcaaca caaaccctga
     8701 aacagggccg tgcgaagacc atgaaaattg aagtccaggg caaagccgcg ccgggcatta
     8761 ccgcaaaaga tatcgtgctg gcaattatcg gtaaaactgg cagcgcaggc ggcaccgggc
     8821 atgtggtgga gttttgcggc gaagcaattc gtgatttaag catggaaggt cgtatgaccc
     8881 tgtgcaatat ggcgatcgaa atgggcgcaa aatccggtct ggttgctcct gacgaaacca
     8941 cctttaacta tgtcaaaggc cgtctgcatg cgccgaaagg caaagatttc gacgacgccg
     9001 ttgcctactg gaaaaccctg caaaccgacg aaggcgcaac tttcgatacc gttgtcactc
     9061 tgcaagcaga agaaatttca ccgcaggtca cctggggcac caatcccggc caggtgattt
     9121 ccgtgaacga caatattccc gatccggctt cgtttgccga tccggttgaa cgtgcgtcgg
     9181 cagaaaaagc gctggcctat atggggctga aaccgggtat tccgctgacc gaagtggcta
     9241 tcgacaaagt gtttatcggt tcctgtacca actcgcgcat tgaagattta cgcgcggcag
     9301 cggagatcgc caaagggcga aaagtcgcgc caggcgtgca ggcactggtg gttcccggct
     9361 ctggcccggt aaaagcccag gcggaagcgg aaggtctgga taaaatattt attgaagccg
     9421 gttttgaatg gcgcttgcct ggctgctcaa tgtgtctggc gatgaacaac gaccgtctga
     9481 atccgggcga acgttgtgca tccaccagca accgtaactt tgaaggccgc caggggcgcg
     9541 gcgggcgcac gcatctggtc agcccggcaa tggctgccgc tgctgctgtg accggacatt
     9601 tcgccgacat tcgcaacatt aaataaggag cacaccatgg cagagaaatt tatcaaacac
     9661 acaggcctgg tggttccgct ggatgccgcc aatgtcgata ccgatgcaat catcccgaaa
     9721 cagtttttgc agaaagtgac ccgtacgggt tttggcgcgc atctgtttaa cgactggcgt
     9781 tttctcgatg aaaaaggcca acagccaaac ccggacttcg tgctgaactt cccgcagtat
     9841 cagggcgctt ccattttgct ggcacgagaa aacttcggct gtggctcctc gcgtgagcac
     9901 gcgccctggg cattgaccga ctacggtttt aaagtggtga ttgcgccgag ttttgctgac
     9961 atcttctacg gcaatagctt taacaaccag ctgctgccgg tgaaattaag cgatgcagaa
    10021 gtggacgaac tgtttgcgct ggtgaaagcc aatccgggga tccatttcga cgtggatctg
    10081 gaagcgcaag aggtgaaagc gggagagaaa acctatcgct ttaccatcga tgccttccgc
    10141 cgccactgca tgatgaatgg tctggacagc atcggcctga ccctgcagca cgacgacgcg
    10201 attgcgtctt atgaagcaaa acaacctgcg tttatgaatt agcccccttg cccggtcaaa
    10261 tgaccgggct ttccgctatc gtccacgtca tcaaacgtct ttaacctttg cggttaaaaa
    10321 taatgcgaca acagaaataa tcgcaattac ccagtagaca gcaaagtgcc cccaactttg
    10381 tgcaattgct ccctgaataa caccagccag aattaccccg gtagagatag tgttggtaaa
    10441 taaggtggta gctgcccccg ctcttccagg cattaaatcc tgaaaccata gcatcccaat
    10501 acccgcaaca atgccgataa atacagcgtt aaaaagttgc agcgtcatca acgccatacg
    10561 gctatgaaag aaaatcaatc cagtgtaaaa cagcactcct gccaccactg caatgaccat
    10621 cattcgccgc ttaccataac gtttgacata gtagccagcc agaatcattg ctggtatttc
    10681 cagtccagct gccgtcccca tcaggaaacc cgccagtttg tctggcaatc ctaaatcgct
    10741 actgatccac aacggcatat caataatgta catagtgttg caggtccaca ttaacgtcga
    10801 ggcgacaaat aacatccgta cgttactatc ctgccagtca ccttgcattg ataaagcatt
    10861 ttccgacggc agttctaccc gcgccacaga cggaagcata aatgcaatca ataccagact
    10921 gagtgtgaat atcccggcgg caatcgaaaa catcactgta aagccgtaat tcaacgccag
    10981 cataaaggcc agcggtggac cgattaccca tgccagagaa agctgcgcac gcatcaccga
    11041 gctaaacatc accacttctc gcgccgagtt atccgcgtat tcccgcgcca gagcaaataa
    11101 ctgtggcatt gccgtattgg ccagagatgc cagaagcaca ccacaggtga taagcgtcag
    11161 ataatgacga ttaaatgcaa acagtagcgc attgccaata gccatcaaac agcaaaatat
    11221 aatcagtttt cgccgatcgc cctgactgtc agaacgtttt gccaaccaga ggcttacgcc
    11281 gatcccagca atagcattca ccgtataaaa gagtccgatc cagaaaggtt gcgcgccaac
    11341 ctcacgactc agaaataagc tcaatgtagg agcctgtagc gccccggcca cccccatcat
    11401 aaaagcgacc agcataaatg ccgcgtaaac accgttcata cggcgagcca tcgtcattat
    11461 ccagatcata cgttcccttt ttagcgcggc gagaataaaa aaaaccagca ggtataatct
    11521 gctggcgggt gattttacac caatactcag tcacacatga tgcaggcaag tcaactttca
    11581 gaattgcggt catcccactg catcagtcct tcaagcatgg ttaatcgttg tggacgctta
    11641 accaacgcaa ccagcacaac ttcgctgtcg cggtaaaata gtgctgataa aactgacgca
    11701 tggggcatct ccttgcttca tcgttaggaa ttattgactt aatataggga aaataaaatt
    11761 gctgtctttt gcgcaggagt tcccctttta tgccatctgc tcgtctgcaa caacagttca
    11821 tccgcctgtg gcaatgctgc gagggtaaat cgcaggacac aacgctcaac gaactggcag
    11881 cgttattgag ctgctcgcgt cgtcatatgc gtaccctgct caacaccatg caggatcgcg
    11941 gctggctgac gtgggaagcg gaagtcgggc gcggtaaacg ctcgcgtctg acattcctct
    12001 ataccgggct ggcgcttcag caacagcggg cggaagacct gctggagcag gatcgtatcg
    12061 accaactagt gcagttggtt ggcgacaaag cgactgtgcg gcaaatgctg gtttctcatc
    12121 tgggccgcag cttccgccag gggcggcaca tcctgcgcgt gctctactat cgtccgttgc
    12181 gtaatctgct acctggcagc gcattgcgcc gttccgaaac ccatattgcc cggcaaatct
    12241 tcagttcgct aacgcgcata aatgaggaaa atggggaact ggaagcagac atcgcccacc
    12301 actggcagca aatatcaccg cttcactggc gtttcttttt gcgtccagga gtccattttc
    12361 atcatggtcg tgaactggaa atggacgacg tgatcgcctc tttaaaacga atcaatacgc
    12421 tgccgctcta ttcgcatatt acggacattg tgtcgccgac gccctggacg ctggatatcc
    12481 atctcacgca gccggaccgc tggttgccgt tactgctggg gcaagttccg gcgatgatcc
    12541 tgccgtgcga atgggaaacc ctcagtaact ttgccagcca tcctattggc accggtccgt
    12601 atgcggtgat tcgcaacagc accaatcaac tgaaaattca ggcattcgat gacttcttcg
    12661 gttaccgggc attaatcgac gaagttaacg tctgggttct gccggaaatt gccgacgagc
    12721 cagccggtga tctgatgcta aaaggtccac agggcgagga aaaagagatt gaaagccgcc
    12781 tggaggaagg ttgctactat ttactgttcg acagccgcac ccatcgcggg gcgaatcagc
    12841 aagtcaggga ctgggtaagc tatgtgcttt ctccaactaa tctggtctat ttcgctgagg
    12901 aacagtacca gcaactgtgg ttcccggctt atggactgct cccccgttgg caccatgccc
    12961 gcaccataaa gagcgaaaaa ccggctggcc tggaaagcct caccctgacc ttttatcagg
    13021 atcacagtga gcatcgggtg attgccggga tcatgcagca gattctggca agtcaccagg
    13081 tcacgctgga aatcaaagag atcagctacg atcagtggca tgaaggagag atcgagagcg
    13141 atatctggct taatagcgcc aactttacgc tgccgctgga tttttcgctg ttcgcgcact
    13201 tgtgcgaggt gccgctgctc caacactgta ttccgatcga ctggcaagcc gacgccgccc
    13261 gctggcgcaa tggcgaaatg aacctggcga actggtgcca gcaactggtc gccagtaaag
    13321 caatggtgcc gcttatccac cactggctga tcattcaggg acaacgcagt atgcgcggcc
    13381 tgcgtatgaa taccctcggc tggttcgatt ttaaatcagc gtggtttgcg ccaccggatc
    13441 catgattgct ggtagataac aaaatcacta cactaacgcc gttctcaacg gggtgccacg
    13501 caaacgcgtg cgctgagaaa atacccgtcg aacctgatcc ggataacgcc ggcgaaggga
    13561 tttgaggctc cttctcaagt cctttgccac tcttttttga ggtgcaaagt gttaaaaaaa
    13621 tgtcttcccc tgctgttgct gtgcacagcg cccgttttcg ctaaacccgt tctgactgtt
    13681 tatacctacg attccttcgc cgccgactgg gggcctggtc caaagatcaa aaaagctttt
    13741 gaagccgact gtaactgcga actgaaactg gtggcgctgg aagatggcgt ttcgcttctc
    13801 aaccgtctac ggatggaagg caaaaatagt aaagccgatg tggtgctggg gctggataac
    13861 aatctgttag acgccgccag taaaaccgga ctatttgcca aaagcggtgt ggcagcggat
    13921 gccgttaacg ttcccggcgg ctggaataat gacactttcg tcccgtttga ttacggctat
    13981 ttcgccttcg tctatgacaa gaacaaactg aaaaacccgc cgcaaagcct gaaagaattg
    14041 gttgagagcg atcagaactg gcgggtgatt tatcaggatc cgcgtaccag cacgccgggg
    14101 ctgggtctgc tgttgtggat gcaaaaagtc tatggcgatg acgccccaca agcctggcaa
    14161 aaactggcga agaaaaccgt cacggtcacc aaaggctgga gcgaagccta cggcctgttt
    14221 ttaaaaggtg aaagcgatct ggtactgagt tacaccacct ctccggctta tcacattctc
    14281 gaagagaaaa aagataacta cgccgccgcg aactttagcg aaggtcacta tctgcaggtg
    14341 gaagtcgccg cccgcaccgc tgccagcaag cagccggagc tggcgcaaaa attcctccag
    14401 tttatggttt ctccggcttt tcagaatgcg atcccaaccg gtaactggat gtatccggtg
    14461 gcaaatgtca cgctgcctgc cgggtttgaa caattgacca aaccggccac cacgctggag
    14521 ttcacaccag ctgaagtggc ggcacaacgt caggcatgga ttagcgaatg gcaacgcgcc
    14581 gtcagccgtt aattcccggc tggttaattc caggtgtaag cgccgccacg ctggtggtag
    14641 cggttgcgct cgcggcgttt ctcgccctgt ggtggaacgc gccgcagggt aactgggtgg
    14701 cagtctggca ggacagctac ctgtggcatg tggtgcgctt ctccttctgg caggcgtttc
    14761 tctcggcgct actctctgtc gtacccgcga tattcctcgc ccgcgccctc tatcgcaggc
    14821 gctttccggg tcggctggcg ctgttgcgtc tgtgcgcaat gaccttgatc ctcccggtgc
    14881 tagttgctgt tttcggcatt cttagcgtct atggtcgtca gggctggctg gcatcgctct
    14941 gccaatcgct cggtctggag tggacctttt cgccctacgg cctgcaaggc attttgctgg
    15001 cgcacgtatt ttttaatctg ccgatggcga gccgcttatt actccaggca ctggaaaaca
    15061 ttcccggcga acaacgtcag cttgccgccc agcttgggat gcgcggctgg cattttttcc
    15121 gcttcgtcga atggccgtgg ctacggcgac aaatcccgcc ggttgccgcg cttatcttta
    15181 tgctctgttt cgccagcttc gccaccgtgc tatcgctggg cggcggtccg caggcaacca
    15241 ctatcgagct ggctatttat caggcgctga gttacgacta cgatcctgcc cgcgcggcga
    15301 tgctggcgct gctccagatg gtgtgttgcc ttgggctggt gctgttgagt cagcgactaa
    15361 gtaaggccat tgcgcccggc accacgctgc tgcaaggctg gcgcgatccg gacgatcgtc
    15421 tgcatagccg catttgcgac acggtgttaa tagtgctggc gctgctgctg ttgctgccac
    15481 cgttgctggc ggtgatcgtc gatggggtaa atcgccagtt gccggaagtg ctggcacaac
    15541 cggtgctgtg gcaggcgctg tggacctcgt tgcgtattgc gctggcggca ggtgtattgt
    15601 gcgtagtgct gaccatgatg ctgctatgga gcagtcgcga actgcgggcg cggcagaaaa
    15661 tgctggcggg tcaggcgctg gagatgagcg gcatgttgat cctcgccatg ccggggattg
    15721 tgctggctac cggcttcttt ttactgctca acaacactat cggcctgcca caatctgctg
    15781 acggcattgt gattttcacc aatgcgttaa tggcgatccc ttatgcgctg aaagtactgg
    15841 aaaacccgat gcgcgatatc accgcccgct acagtatgtt gtgtcagtcg ctgggcattg
    15901 aaggctggtc gcgcttaaaa gtggtcgagc tgcgcgccct gaaacgtcca ctggcgcagg
    15961 cgctggcttt tgcctgcgtg ctgtcgattg gtgattttgg cgtagtggcg ttgttcggta
    16021 acgatgattt ccgtaccctg ccgttttatc tctaccagca aattggctcc tatcgcagcc
    16081 aggacggcgc ggtcaccgcg ttaattctgc tactactctg ttttctgctg tttaccgtga
    16141 ttgaaaaact accggggcga aatgttaaaa ctgactgata tcacctggct ttatcaccat
    16201 ttgccgatgc gttttagctt aacggtagaa cgcggcgagc aggtggcaat cctcgggcca
    16261 agcggcgcgg gtaaaagtac cctgctgaat ttgattgccg gttttctgac gccagccagc
    16321 ggttcgctga ctatcgatgg cgttgatcac accacgacgc cgccgtcacg ccgtccggtg
    16381 tcgatgctgt ttcaggagaa caacctgttc agccacctga cggtcgcgca gaacatcggg
    16441 ctggggctaa atccggggtt gaaactgaac gcggcacagc aggagaaaat gcacgcgatt
    16501 gcccgtcaga tggggattga taatttaatg gcgcggttac cgggcgagct ttccggcggt
    16561 cagcgacagc gcgtggcgtt agcgcgttgt ctggtgcgcg aacagccgat tttattgctc
    16621 gatgaaccgt tctctgcgct cgatccggcg ttacgtcagg agatgctgac gctggtgagc
    16681 acgagctgcc agcagcaaaa aatgacgcta ttgatggtgt cgcacagtgt ggaagatgcg
    16741 gcgcggatcg ccacacgctc ggtggtggtc gccgacgggc gcatcgtctg gcagggtaag
    16801 actgaagaat tgttgagcgg taaggcgagt gcttcggcaa tattggggat tacgggttag
    16861 tatgcactgt cagatgcggc gtgaacacct tatccgacct acggacagtg cgttttgtag
    16921 gcctgataag acgcggtcag cgtcgcatga ggcaacccgc acaagacaat cccctaaacc
    16981 ccaaccactt tacgcaaaat atcgatatac accggcatca acgggtggcg aattaacacc
    17041 accagcgcca ccacgccgat ggcagaaatc aacggcgtca gccacaacaa acggccgcgc
    17101 gacaaataat gacttaaacg atcggttgct ttaccgctgc gccataaccg ccagcacagc
    17161 cagccaccaa cccacaaaaa taccgccgtc gccagcagca accatttaaa ctcgccgctc
    17221 tgcattccgg ctggaatatc gatcgccgcg cccgccagaa tcccaggcag gaagtaaaac
    17281 ggtggccaca gcaggcagcc gataatattc ggcgtaatga attttgccac cggcagatcc
    17341 agcattcccg ccaccattgg caccagcgga cgcgtcgggc caacaaagcg accgaccaga
    17401 atggtgaaca tgctgtgttg atgcaacgca tgttcagttt tatcgagtaa tgctttgttt
    17461 ttcttcagaa atgaccagcg atgcaacggc tttttaaaac gccagcccag ccagaaggag
    17521 atccagtcgc ccagcagaca accgacaatc cctgccagcc aagcgtgcca gaaacttaac
    17581 tcgccgctgc caatcagcgc tcccagcccc gccatcagca ccgtaccggg tagaatcaaa
    17641 ccgaccagcg ccagcgactc cagaaaggcc accaacacca ccgccatcaa tgaatacacg
    17701 gtagattggg taataaagtg ttccagcaat gcttgcataa tgtgcctgtc aaatggacga
    17761 agcagggatt ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct gattcgttac
    17821 caattatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg cacggaactc
    17881 gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt gatcgtcaaa
    17941 accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc
    18001 ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa
    18061 aagatgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa
    18121 attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtacac gattatccat
    18181 cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt gctcaagcag
    18241 atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa tgatttgccc
    18301 aaacaggtcg ctgaaatacg gctggtgcgc ttcatccggg cgaaagaaac ccgtattggc
    18361 aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa agtaaaccca
    18421 ctggtgatac cattcgcgag cctccggatg acaaccgtag tgatgaatct ctcctggcgg
    18481 gaacagcaaa atatcacccg gtcggcagac aaattctcgt ccctgatttt tcaccacccc
    18541 ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc ggtcgataaa
    18601 aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat gggcattaaa
    18661 cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca tactcccgcc
    18721 attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta
    18781 ctggctcttc tcgctaaccc aaccggtaac cccgcttatt aaaagcattc tgtaacaaag
    18841 cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt
    18901 ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag
    18961 attagcggat cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt
    19021 tttttggatg gagtgaaacg atggcgattg caattggcct cgattttggc agtgattctg
    19081 tgcgagcttt ggcggtggac tgcgccagcg gtgaagagat cgccaccagc gtagagtggt
    19141 atccccgttg gcaaaaaggg caattttgtg atgccccgaa taaccagttc cgtcatcatc
    19201 cgcgtgacta cattgagtca atggaagcgg cactgaaaac cgtgcttgca gagcttagcg
    19261 tcgaacagcg cgcagctgtg gtcgggattg gcgttgacag taccggctcg acgcccgcac
    19321 cgattgatgc cgacggtaac gtgctggcgc tgcgcccgga gtttgccgaa aacccgaacg
    19381 cgatgttcgt attgtggaaa gaccacactg cggttgaaga agcggaagag attacccgtt
    19441 tgtgccacgc gccgggcaac gttgactact cccgctatat tggcggtatt tattccagcg
    19501 aaggttctgg gcaaaaatcc tgcatgtgac tcgccaggac agcgccgtgg cgcaatctgc
    19561 cgcatcgtgg attgagctgt gcgactgggt gccagctctg ctttccggta ccacccgccc
    19621 gcaggatatt cgtcgcggac gttgcagcgc cgggcataaa tctctgtggc acgaaagctg
    19681 gggcggcttg ccgccagcca gtttctttga tgagctggac ccgatcctca atcgccattt
    19741 gccttccccg ctgttcactg acacctggac tgccgatatt ccggtgggca ccttatgccc
    19801 ggaatgggcg cagcgtctcg gcctgcctga aagcgtggtg atttccggcg gcgcgtttga
    19861 ctgccatatg ggcgcagttg gcgcaggcgc acagcctaac gcactggtaa aagttatcgg
    19921 tacttccacc tgcgacattc tgattgccga caaacagagc gttggcgagc gggcagttaa
    19981 aggtatttgc ggtcaggttg atggcagcgt ggtgcctgga tttatcggtc tggaagcagg
    20041 ccaatcggcg tttggtgata tctacgcctg gtttggtcgc gtactcggct ggccgctgga
    20101 acagcttgcc gcccagcatg cggaactgaa agcgcaaatc aacgccagcc agaaacaact
    20161 gcttccggcg ctgaccgaag catgggccaa aaatccgtct ctggatcacc tgccggtggt
    20221 gctcgactgg tttaacggtc gtcgcacgcc aaacgctaac caacgcctga aaggggtgat
    20281 taccgatctt aacctcgcta ccgacactcc gctgctgttc ggcggtttga ttgctgccac
    20341 cgcctttggc gcacgcgcaa tcatggagtg ctttaccgat caggggatcg ccgtcaataa
    20401 cgtgatggcg ctgggcggca tcgcgcggaa aaaccaggtc attatgcagg cctgctgcga
    20461 cgtgctgaat cgcccgctgc aaattgttgc ctctgaccag tgctgtgcgc tcggtgcggc
    20521 gatttttgct gccgtcgccg cgaaagtgca cgcagacatc ccatcagccc agcaaaaaat
    20581 ggccagtgcg gtagagaaaa ccctgcaacc gcgcagcgaa caggcacaac gctttgaaca
    20641 gctttatcgc cgctatcagc aatgggcgat gagcgccgaa caacactatc ttccaacttc
    20701 cgccccggca caggctgccc aggccgttgc gactctataa ggacacgata atgacgattt
    20761 ttgataatta tgaagtgtgg tttgtcattg gcagccagca tctgtatggc ccggaaaccc
    20821 tgcgtcaggt cacccaacat gccgagcacg ttgttaatgc gctgaatacg gaagcgaaac
    20881 tgccctgcaa actggtgttg aaaccgctgg gcaccacgcc ggatgaaatc accgctattt
    20941 gccgcgacgc gaattacgac gatcgttgcg ctggtctggt ggtgtggctg cacaccttct
    21001 ccccggccaa aatgtggatc aacggcctga ccatgctcaa caaaccgttg ctgcaattcc
    21061 acacccagtt caacgcggcg ctgccgtggg acagtatcga tatggacttt atgaacctga
    21121 accagactgc acatggcggt cgcgagttcg gcttcattgg cgcgcgtatg cgtcagcaac
    21181 atgccgtcgt taccggtcac tggcaggata aacaagccca tgagcgtatc ggctcctgga
    21241 tgcgtcaggc ggtctctaaa caggataccc gtcatctgaa agtctgccga tttggcgata
    21301 acatgcgtga agtggcggtc accgatggcg ataaagttgc cgcacagatc aagttcggtt
    21361 tctccgtcaa tacctaggcg gttggcgatc tggtgcaggt ggtgaactcc atcagcgacg
    21421 gcgatgttaa cgcgctggtc gatgagtacg aaagctgcta caccatgacg cctgccacac
    21481 aaatccacgg cgaaaaacga cagaacgtgc tggaagcggc gcgtattgag ctggggatga
    21541 agcgtttcct ggaacaaggt ggcttccacg cgttcaccac cacctttgaa gatttgcacg
    21601 gtctgaaaca gcttccaggt ctgcccgtac agcgtctgat gcagcagggt tacggctttg
    21661 cgggcgaagg cgactggaaa accgccgccc tgcttcgcat catgaaggtg atgtcaaccg
    21721 gtctgcaggg cggcacctcc tttatggagg actacaccta tcacttcgag aaaggtaatg
    21781 acctggtgct cggctcccat atgctggaag tctgcccgtc gattgccgta gaagagaaac
    21841 cgatcctcga cgttcagcat ctcggtattg gtggtaagga cgatcctgcc cgactgatct
    21901 tcaataccca aaccggtcca gcgattgtcg ccagcctgat tgatctcggc gatcgttacc
    21961 gtctgctggt taactgtatc gacacggtga aaacaccgca ctccctgccg aaactgccgg
    22021 tggcgaatgc actgtggaaa gcgcaaccgg atctgccaac tgcttccgaa gcgtggatcc
    22081 tcgctggtgg cgcgcaccat accgtcttca gccatgcgct gaacctcaac gatatgcgcc
    22141 agttcgccga gatgcacgac attgaaatca cggtgattga taacgacacc cgcctgccag
    22201 cgtttaaaga cgcactgcgc tggaacgaag tgtattacgg atttagtcgc taagtagcca
    22261 catcaggtat gtaacgcctg atgcgacgct gactcgtctt atctggccta cacgctgcga
    22321 ttttgtaggc cggataagca aagcgcatcc ggcattcaac gcctgatgcg acgctggcgc
    22381 gtcttatcag gcctacgcgc tgcgattttg tagaccggat aagcgaagcg catccggcac
    22441 gaaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa cctggcgctg
    22501 ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgacgttga tcgcgagcgc
    22561 ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc tgacgatatg
    22621 gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc ctcctccgac
    22681 acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat tgtgcatacg
    22741 cactcgcgcc acgccaccat ctgggcgcag gcgggtcagt cgattccagc aaccggcacc
    22801 acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac cgacgcagaa
    22861 atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt tgaaaaacag
    22921 ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc gtttgcatgg
    22981 ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt cgcttatatg
    23041 gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac gctgctggat
    23101 aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg actgtataaa
    23161 accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga tggattttca
    23221 gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg caagggacag
    23281 aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt gcaccgcaag
    23341 agtccgtggc gtttattcac gccgatcagg ttccccgcgc tcagcatatt ttgcagggtg
    23401 aacaaggctt tcgtctgaca ccgctggcgt taaaggattt tcaccgccag ccggtgtatg
    23461 gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg cgtgaaggtg
    23521 gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg gagcggttta
    23581 tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt aatgcccgtc
    23641 tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat attgaaacca
    23701 cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc atcgtttata
    23761 tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa tacgtcgcca
    23821 gccgcccgct gctgctggaa aaactcaacg cctggtttgc aaaccacgat cccgatgtga
    23881 tcatcggctg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat gccgagcgtt
    23941 accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc gagcacggct
    24001 ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac ggtatcgagg
    24061 cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc gctcaggagc
    24121 tattaggcga aggaaaatct atcgataacc cgtgggttcg aatggacgaa attgaccgcc
    24181 gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc gagctggtga
    24241 cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca acggtgaacg
    24301 gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc tattttccgc
    24361 gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg cacgccagcc
    24421 ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg gtgctggact
    24481 ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc gggctggttg
    24541 aaggcatggc gcaacctgat ccagagcaca gtaccgaagg ttttctcgat gcctggttct
    24601 cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg cgcgatgaag
    24661 ccaaacgtca gggtaataag ccgctttcgc aggcgctgaa gatcattatg aatgcctttt
    24721 atggcgtgct cggcaccacc gcctgccgct tcttcgatcc gcgcctggca tcgtcgatca
    24781 ccatgcgtgg tcatcagatc atgcggcaga ccaaagcatt gattgaagcg cagggctacg
    24841 acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca cattcggaag
    24901 aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg tgggcggaaa
    24961 cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact catttctgcc
    25021 gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc tacgccgggc
    25081 tgattcagga gggcgacaag cagcggatgg tgtttaaagg gctggaaacc gtgcgcaccg
    25141 actggacgcc gctggcccag cagtttcagc aggagctgta cctgcgtatc ttccgcaacg
    25201 agccatatca ggagtacgtc cgccaaacca tcgacaaact gatggcgggt gaactggatg
    25261 cgcggctggt ttaccgtaaa cgccttcgcc gtccgctgag cgaatatcag cgtaatgttc
    25321 cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa tcaaaagcgc ggtcgcccct
    25381 tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc ccggagccgc
    25441 tggactacca acgttcacca ctggattacg aacactatct gacccgccag ctacaacccg
    25501 tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg accgggcaac
    25561 ttgggctatt ttgagcaaaa aaaagagttc gccagatacc attttgatgc gtgacgaatg
    25621 ctttgccatc cagtaccata gcgccctttc cattcctgga cctgaataac accactacct
    25681 cataagcacg gtagcgggtg gttattgcct gcaattaaag atatagagcc gaacacatat
    25741 gccttttaca cttggtcaac gctggatcag cgatacagaa agcgaattgg gacttggaac
    25801 cgttgtcgcg gtggatgcgc gaactgtcac tttacttttc ccatctactg gtgaaaaccg
    25861 tctgtacgca cgcagtgatt cccccgtgac ccgcgtgatg ttcaaccctg gtgataccat
    25921 taccagccat gacggctggc agatgcaagt cgaagaagta aaagaagaaa atggcttgct
    25981 gacctatatc ggtactcgcc tggatactga agagtccggc gtagccctgc gtgaagtttt
    26041 ccttgatagc aaactggtgt tcagcaaacc gcaggaccgt ctgtttgccg ggcagattga
    26101 ccgtatggac cgctttgcgc tgcgttatcg cgcgcgtaag tattccagcg aacagttccg
    26161 tatgccgtac agcggcctgc gcggtcagcg taccagcctg atcccgcatc agctcaacat
    26221 cgctcatgat gttggtcgcc gccacgcgcc gcgcgtcctg ctggctgacg aagtgggttt
    26281 agggaaaacc attgaagccg ggatgatcct gcatcagcaa ctgctctctg gcgctgctga
    26341 acgtgtgctg attatcgtcc cggaaacctt acagcatcag tggctggtag aaatgctgcg
    26401 ccgtttcaac ctgcgctttg ctctgtttga tgatgagcgt tatgccgaag ctcagcacga
    26461 tgcttacaac ccgtttgaca ccgaacagct ggtgatttgc tcgctggatt ttgcccgtcg
    26521 tagcaaacag cgcctggaac atctctgtga agccgaatgg gacctgctgg tggtcgatga
    26581 agcgcatcac ctggtgtgga gcgaagatgc gccaagccgt gaatatcagg ccattgaaca
    26641 actggcagag cacgtgccgg gcgttctgct gctgaccgcg accccggaac agctggggat
    26701 ggaaagccac ttcgcccgtc tgcgtctgct ggacccgaac cgtttccacg attttgcgca
    26761 gttcgttgaa gagcagaaaa attatcgtcc ggttgcggac gccgttgcca tgctgctggc
    26821 aggtaacaaa ctgagcaatg acgaactgaa catgctcggc gagatgatcg gcgagcagga
    26881 tatcgagccg ctgttgcagg cagcaaacag cgacagcgaa gatgcccaga gcgcccgtca
    26941 ggagctggtt tcgatgctga tggatcgcca cggcaccagc cgcgtgctgt tccgtaacac
    27001 gcgtaacggt gtgaaaggat tcccgaaacg cgagctgcac accattaagc tgccgctacc
    27061 gacgcagtat cagacggcta ttaaagtctc cggcattatg ggcgcacgta aaagtgcgga
    27121 agatcgtgct cgcgatatgc tctacccgga gcgtatttat caggaatttg aaggtgataa
    27181 cgccacctgg tggaacttcg atccgcgcgt tgagtggctg atgggctacc tgaccagcca
    27241 tcgctctcag aaagtgctgg tgatctgcgc caaagctgcc actgcgctgc aactggagca
    27301 ggtactgcgc gaacgtgaag gtattcgcgc tgcggtgttc cacgaaggta tgtcgattat
    27361 cgaacgtgac cgcgctgccg cctggtttgc cgaagaagac accggcgcac aggtactgct
    27421 gtgctcagaa atcggttctg aaggacgtaa ctttcagttc gccagccaca tggtgatgtt
    27481 tgacctgcca ttcaacccgg atctactgga gcagcgtatt ggtcgtctgg atcgtatcgg
    27541 ccaggcgcac gatattcaga tccatgtgcc ttatctggag aaaaccgctc agtcggtgct
    27601 ggtgcgctgg tatcacgaag gtctggatgc atttgagcac acctgcccga ccggacgcac
    27661 tatttacgat agcgtataca acgatctgat taactatctg gcttcaccgg atcaaaccga
    27721 aggctttgac gatctgatca aaagctgccg cgagcaacat gaagcgctga aagcacagct
    27781 ggaacagggt cgtgaccgcc tgctggaaat ccactccaac ggtggcgaaa aagcccaggc
    27841 actggcagaa agcattgaag agcaggatga cgataccaac ctgatcgcct tcgccatgaa
    27901 cctgttcgat attatcggta tcaatcagga cgatcgcggc gacaacatga tcgtgctgac
    27961 gccgtccgat catatgctgg tgccggactt ccctggcctg tcggaagatg gcatcaccat
    28021 cacctttgat cgtgaagtgg cgctggcgcg tgaagatgca cagtttatta cctgggagca
    28081 tccgctgatc cgcaacggtc tggatctgat cctttctggc gataccggta gcagcacgat
    28141 ttcactgtta aaaaacaaag cgttgccggt aggtacgctg ttggtggaac tgatttatgt
    28201 ggttgaagcc caggctccga agcagttgca gctcaaccgc ttcctgccac cgacgccggt
    28261 acgtatgctg ctggataaaa acggcaacaa cctggcggcg caggtagagt ttgaaacctt
    28321 taaccgccag cttaacgcgg ttaaccgtca caccggcagc aaactggtta acaccgtgca
    28381 gcaggatgtt cacgctatcc ttcaactggg tgaagcgcag atcgagaaat ctgcccgtgc
    28441 attgattgat gcagcgcgta acgaagccga cgaaaaactg tctgccgagc tgtctcgtct
    28501 ggaagctctg cgtgcagtga acccgaacat tcgtgacgac gaactgaccg ccattgagag
    28561 caaccgtcag caggtaatgg aaagcctgga tcaggcaggt tggcgtctgg atgccctgcg
    28621 tttgatcgtt gtaacgcatc agtaacggag ccgaaaatgg ggatggaaaa ctacaatcca
    28681 ccgcaggaac cctggttggt tatcctgtat caggatgacc atattatggt ggtcaacaag
    28741 ccgagcggtt tgttgtcagt gccgggtcgt ctggaagagc acaaagacag cgtgatgacg
    28801 cgcattcagc gtgattatcc gcaggcagaa tcggtgcatc gtctggatat ggctaccagc
    28861 ggcgtgattg tagtggcgct gaccaaagcc gcggagcggg agttaaaacg ccagttccgc
    28921 gagcgcgagc cgaaaaagca gtatgtggcc cgcgtctggg ggcatccatc ccccgcagaa
    28981 ggtctggtgg atctgccgct gatttgcgac tggccaaacc gcccgaaaca gaaagtctgt
    29041 tacgaaacgg gtaaacctgc gcagacggaa tatgaagtgg tggagtatgc ggcggataac
    29101 acggcaagag cggtgttaaa accgattacc gggcgttcgc atcaattgcg tgtgcatatg
    29161 ctggcgctgg ggcatccgat tctcggcgat cgtttttatg catcaccaga agcgagagcg
    29221 atggcaccgc gtttgttgct acatgcagag atgttgacga ttacccatcc ggcgtatggc
    29281 aatagtatga cgtttaaagc gccagcggat ttttaatcgt cacgctggcg cagttcggta
    29341 ggcggataag gcgttcacgc cgcatccggc aattgtgcgc cgattgcctg atgcgacgcg
    29401 ggccgcgtct tatcaggcct acagtttaaa acttatttaa accctttctg ctgctttatc
    29461 agctcatatg cctgctgaat ttcctgcgct ttctgcttcg ccatctccat catctcaggc
    29521 ggcaaacctt tcgccaccag cttatcggga tggtgttcac tcatcagctt acggtaggca
    29581 cgtttgatgg tggtcgcatc atccgtcggc ttcacgccca gcacattaca ggcatcttcc
    29641 agcgtcgggc cacgctgcgc ttgctgccag ttaccaccgc cagattgctg ctgataaccg
    29701 ccgccaaact gtgcaccgcc ctgcatcatg cgcaaaaact ggtcaaactg ggcgcgggag
    29761 atccctaatt cttcagcaat gacatacagc accgcccgtt cattcgggtg cagtgaacca
    29821 tcagcaaacg ccgcctgaat ctggatctcc agaaacatac gaattaagtc aaaacgacca
    29881 aagcagacac tgcgaaactg gcgcatcttt tcgcgcagcg ggtaattatc tgattttccc
    29941 acccggaacg cgttttgcgc cgcagtacgg gaagcgccat gaaggttcat tcggtccatc
    30001 aactggctgg cgatatgaat atcagcctcc gtgacgcgac ctttggattt ggttaaatgc
    30061 cccatcactt caaaagtggt ggcaaaaaac agcgcctgac gctcacgctg gttggcgaac
    30121 cacgccattt tacggctacg ggctttatca aacatatggc caattaacag gcctaacact
    30181 acgccccaaa agccgccgcc catcagtaag gccacggcca cgccaatgat ttttccccaa
    30241 tactgcatat attccccaaa tcgacacacg gatatcaggg ctatctccca caatataaag
    30301 gtgcttttac cgttttccgg ctgcggtcaa ttgtgggaca tcgcctataa tttgcattat
    30361 catacctgtc attcaatgcc gtgcctaaca ccactgacgc tattcggaca ggattaacac
    30421 tagcgtagag atgacgagta cgttagtctc tgaccgtttg tcacgcgcaa cgttaccgat
    30481 gatggaacaa taaaatcaac gtatgaaaaa acgtatcccc actctcctgg ccaccatgat
    30541 tgccaccgcc ctttatagtc aacagggact ggcagccgac ctcgcctcac agtgcatgtt
    30601 gggcgtgcca agctatgacc gtcctctggt acagggcgat accaatgact tacccgtgac
    30661 tatcaatgct gaccacgcga aaggggacta cccggatgac gccgtgttta ctggcagcgt
    30721 ggatatcatg cagggtaaca gccgtctgca ggccgacgaa gtgcagctcc atcaaaaaga
    30781 ggcaccagga caaccggagc cggtacgtac cgttgatgcg ctcggtaatg tccattacga
    30841 cgataaccag gtgatcctca aagggccgaa aggctgggcg aatctgaaca ccaaagatac
    30901 caacgtctgg gaaggtgatt accagatggt gggtcgccag ggtcgcggta aagcggacct
    30961 gatgaaacaa cgtggcgaaa accgctatac cattctggat aacggtagct ttacctcctg
    31021 tctgccgggt tctgacacct ggagcgtggt aggtagcgaa attattcatg accgcgaaga
    31081 acaagttgcg gagatctgga acgcccgctt taaggtgggt ccggtaccga tcttttatag
    31141 cccctatttg cagttgccgg tgggtgacaa acgtcgctct ggtttcttga tcccgaacgc
    31201 caagtacacc accaccaact actttgagtt ctacctgcca tattactgga acatcgcgcc
    31261 aaatatggat gccaccatca cgccgcatta tatgcatcgt cgtggcaaca tcatgtggga
    31321 gaacgaattc cgctacctct cccaggcggg cgctggcttg atggaactgg actatctgcc
    31381 ttcagataaa gtctatgaag atgaacaccc gaacgatgac agttcacgtc gttggttatt
    31441 ctactggaac cactccgggg tcatggatca ggtgtggcgt ttcaacgtcg actacaccaa
    31501 ggtcagcgat cctagctact tcaatgattt cgataacaag tacggttcca gtactgacgg
    31561 ctacgcaacg caaaaattca gcgttggcta tgcggtgcaa aacttcaatg ccaccgtttc
    31621 aaccaagcag ttccaggttt tcagcgaaca gaacaccagt agctactcgg cagagccgca
    31681 gttagacgtt aattactacc agaatgatgt tggtccgttt gatacgcgta tttacggcca
    31741 ggcagtgcac tttgttaaca ccagagacga catgcctgaa gcaacccgtg ttcacctgga
    31801 accgaccatc aatttgccgc tctctaataa ctggggcagc atcaataccg aagcgaagtt
    31861 tctggcaacc cattatcagc aaaccaatct tgactggtat aactccagaa acacgaccaa
    31921 gctggacgaa tccgttaacc gcgtaatgcc gcaattcaaa gttgacggca aaatggtctt
    31981 tgaacgcgat atggaaatgc tggctccggg ttatacccaa acgctggaac cgcgcgcgca
    32041 gtatttgtac gtgccgtatc gcgatcagag cgacatctat aactacgact cgtctctgct
    32101 gcaatctgac tactctggcc tgttccggga ccggacttac ggcggtcttg accgtattgc
    32161 ctccgctaac caggtgacga ccggtgtcac atctcgcata tatgatgatg ctgccgttga
    32221 acgttttaat atttccgttg gtcaaatcta ctatttcacg gagtctcgca ctggcgatga
    32281 caacataaca tgggagaatg acgacaaaac gggctcactg gtgtgggcag gcgacaccta
    32341 ctggcgtatc tccgagcgtt ggggattgcg tggcgggatt cagtacgata cacgtctgga
    32401 taacgtagcg accagtaact ccagcattga ataccgtcgg gatgaagacc gtctggtaca
    32461 gctgaattac cattacgcca gcccggaata tattcaggct acgctgccta agtactattc
    32521 cactgctgag caatataaga atggtatttc gcaggtaggt gctgtcgcca gcaggccaat
    32581 tgccgatcgt tggtccattg ttggggccta ctactacgac accaatgcga acaagcaagc
    32641 cgactctatg ttaggtgtgc aatacagctc ctgctgctat gcaattcgcg tcggttacga
    32701 gcggaagctg aacggttggg ataacgataa acaacatgcg gtatatgaca acgcaatcgg
    32761 ctttaacatc gaacttcgcg gcctgagctc caactacggt ctgggtacgc aagagatgct
    32821 gcgttcgaac attctgccgt atcaaaacac tttgtgattt gttgatttac cacgtaatcc
    32881 gcagtgcggt taattgaaat ggaaaaagta tgaagaactg gaaaacgctg cttctcggta
    32941 tcgccatgat cgcgaatacc agtttcgctg ccccccaggt agtcgataaa gtcgcagccg
    33001 tcgtcaataa cggcgtcgtg ctggaaagcg acgttgatgg attaatgcag tcggtaaaac
    33061 tgaacgctgc gcaggcaagg cagcagcttc ctgatgacgc gacgctgcgc caccaaatca
    33121 tggaacgttt gatcatggat caaatcatcc tgcagatggg gcagaaaatg ggagtgaaaa
    33181 tctccgatga gcagctggat caggcgattg ctaacatcgc gaaacagaac aacatgacgc
    33241 tggatcagat gcgcagccgt ctggcttacg atggtctgaa ctacaacact tatcgtaacc
    33301 agatccgcaa agagatgatt atctctgaag tgcgtaacaa cgaggtgcgt cgtcgcatca
    33361 ccatcctgcc gcaggaagtc gaatccctgg cgcagcaggt gggtaaccaa aacgacgcca
    33421 gcactgagct gaacctgagc cacatcctga tcccgctgcc ggaaaacccg acctctgatc
    33481 aggtgaacga agcggaaagc caggcgcgcg ccattgtcga tcaggcgcgt aacggcgctg
    33541 atttcggtaa gctggcgatt gctcattctg ccgaccagca ggcgctgaac ggcggccaga
    33601 tggggtgggg ccgtattcag gagttgccgg ggatcttcgc ccaggcatta agcaccgcga
    33661 agaaaggcga cattgttggc ccgattcgtt ccggcgttgg cttccatatt ctgaaagtta
    33721 acgacctgcg cggcgaaagc aaaaatatct cggtgaccga agttcatgct cgccatattc
    33781 tgctgaaacc gtcgccgatc atgactgacg aacaggcccg cgtgaaactg gagcaaattg
    33841 ctgctgatat caagagtggt aaaacgactt ttgctgccgc agcgaaagag ttctcgcagg
    33901 atccaggttc tgctaaccag ggcggtgatc tcggctgggc tacaccagat attttcgatc
    33961 cagccttccg tgacgccctg acccgcctga acaaaggtca aatgagtgca ccggttcact
    34021 cttcattcgg ctggcattta atcgaactgc tggatacccg taatgtcgat aaaaccgacg
    34081 ctgcgcagaa agatcgtgca taccgcatgc tgatgaaccg taagctctcg gaagaagcag
    34141 caagctggat gcaggaacaa cgtgccagcg cctacgttaa aatcctgagc aactaatggt
    34201 taaaacccaa cgtgttgtga tcactcccgg cgagcccgcc gggattggcc cggacttagt
    34261 tgtccagctt gcacagcgtg agtggccggt cgaactggtt gtttgtgccg atgccactct
    34321 ccttaccgac cgggcagcga tgctcggttt gccgctcacc ctccgccctt attcccccaa
    34381 ctcccctgca caaccgcaaa ctacgggcac attaacgcta cttcctgtcg cgctacgtga
    34441 atctgtcact gcggggcagt tagcgattga aaatgggcat tacgtggtgg agacgctggc
    34501 gcgagcgtgt gatggctgtc tgaacggtga atttgctgcg ctgatcacag gccccgtgca
    34561 taaaggcgtc attaacgacg caagcattcc gtttaccggt cataccgagt ttttcgaaga
    34621 gcgttcgcag gcgaaaaaag tggtgatgat gctggcgacc gaagaacttc gcgtggcgct
    34681 ggcaacgacg catttaccgc tgcgcgatat cgcagatgct atcacccctg cacttttgca
    34741 cgaagtgatt gctattttgc atcacgattt gcgtacaaaa tttggtatta ccgaaccgcg
    34801 cattctggtc tgcgggctga atccgcacgc gggcgaaggc ggtcatatgg gtacggaaga
    34861 gatagacacc attattccgg tgctcgacga gctgcgggcg caggggatga aactcaacgg
    34921 gccgctgcct gccgataccc tgtttcagcc gaaatatctc gataacgccg acgccgtgct
    34981 ggcgatgtac cacgatcagg gtcttcccgt gctaaaatac cagggcttcg ggcgcggtgt
    35041 gaacattacg ctgggcctgc cctttattcg cacatcagtg gaccacggca ccgcgcttga
    35101 actggcggga cgtggcgaag ccgatgtcgg cagttttatt acggcgctta atctcgccat
    35161 caaaatgatt gttaacaccc aatgaataat cgagtccacc agggccactt agcccgtaaa
    35221 cgcttcgggc aaaactttct caacgatcag ttcgtgatcg acagcattgt ttctgccatt
    35281 aacccgcaaa agggccaggc aatggtcgaa atcggccccg gtcttgcggc attgaccgag
    35341 ccggtcggcg aacgtctgga ccagctgacg gtcatcgaac ttgaccgcga tctggcggca
    35401 cgtctgcaaa cgcatccatt cttaggcccg aaactgacga tttatcagca ggatgcgatg
    35461 acctttaact ttggcgaact ggccgagaaa atgggtcagc cgctgcgtgt tttcggcaac
    35521 ctgccttata acatctccac gccgttgatg ttccatctgt ttagctatac tgatgccatt
    35581 gccgacatgc actttatgtt gcaaaaagag gtggtgaatc gtctggttgc aggaccgaac
    35641 agcaaagcgt atggtcgatt aagcgtcatg gcgcaatact attgcaacgt gatcccggta
    35701 ctggaagttc cgccgtcagc ctttacacca ccacccaaag tggattccgc cgtcgtgcgc
    35761 ctggtccctc atgcaacgat gcctcacccg gttaaagatg ttcgcgtgtt gagccgcatc
    35821 accaccgaag cctttaacca gcgtcgtaaa accattcgta acagcctcgg caacctgttt
    35881 agcgtcgagg tgttaaccgg aatggggatc gacccggcga tgcgagcgga aaatatctct
    35941 gtcgcgcaat attgccagat ggcgaactat ctggcggaga acgcgccttt gcaggagagt
    36001 taacgatgat caattcgccc cgagtgtgta ttcaggttca aagcgtctac attgaggcac
    36061 aatcttcacc tgataatgaa cgttacgttt ttgcttatac cgtaaccata cgcaatctgg
    36121 ggcgagcgcc agtgcagttg ttggggcgtt actggctgat caccaatggc aatggccgtg
    36181 aaaccgaagt ccagggcgaa ggagtggttg gcgtccagcc acttatcgcg cctggcgaag
    36241 agtaccagta caccagcggt gcaatcattg aaaccccgct gggcaccatg cagggtcact
    36301 acgaaatgat cgatgaaaat ggcgtccctt tcagcatcga cattcccgta ttccgactcg
    36361 ccgttcccac actcattcat taaaagaata tggcgacata ccttattggc gacgttcatg
    36421 gttgttacga tgaactgatc gcattgctgc ataaagtaga atttaccccc gggaaagata
    36481 ccctctggct gacgggcgat ctggtcgcgc gcggcccggg ttcgctggat gttctgcgct
    36541 atgtgaaatc cttaggcgac agcgtacgtc tggtgctggg caatcacgat ctgcatctgc
    36601 tggcggtatt tgccgggatc agccgcaata aaccgaaaga tcgcctgaca ccgctgctgg
    36661 aagcgccgga tgccgacgag ctgcttaact ggctgcgtcg ccagcctctg ctgcaaatcg
    36721 acgaagagaa aaagctggtg atggcccacg cagggatcac gccgcagtgg gatctgcaga
    36781 ccgccaaaga gtgcgcgcgc gatgtagaag cggtgctgtc gagtgactcc tatcccttct
    36841 ttcttgacgc tatgtacggc gatatgccaa ataactggtc accggaattg cgggggctgg
    36901 gaagactgcg ttttatcacc aacgccttta cccgtatgcg tttttgcttc ccgaacggtc
    36961 aactggatat gtacagcaaa gaatcgccgg aagaagcccc tgccccactg aaaccgtggt
    37021 ttgcgattcc tggccctgtc gctgaagaat acagcatcgc ctttggtcac tgggcatcgc
    37081 tggaaggcaa aggtatgccg gaaggtattt acgcgctgga taccggctgc tgctggggtg
    37141 ggtcattaac ctgcctgcgc tgggaagata aacagtattt tgtccagccg tcgaaccggc
    37201 ataaggattt gggcgaagcg gcggcgtctt aaacacagcc tgatacagga aggccggata
    37261 agacgcgacc ggcgtcgcat ccggcactag ccgtaaattc tatacaaact taccgccgct
    37321 ccagaatctc aaagcaatag ctgtgagagt tctgcgcatc ggcgtcgtgg aattcgctga
    37381 ataccgattc ccagtcatcc ggctcgtaat ccgggaaatg ggtgtcgcct tccacttctg
    37441 cgtcgatatg cgtcagatac agtttttgcg cttttggcag gaactgctca taaacgcgac
    37501 cgccgccaat caccatgatt tctggtacgt caccacacgc cgcaatggct tcatccaccg
    37561 atttcaccca cgttacgcga tcgtccgtac ccggttgact gctgaggata atatttttgc
    37621 gtcctggcaa cggacgaccg attgattccc aagtatggcg gcccataatc acgggtttat
    37681 ttaaggtgtt gcgtttaaac caggcgagat cggcaggcag gttccacggc atggcgtttt
    37741 ccatgccgat aacgcgatct accgctaacg ccgcaatcag actgatcatt gagatttccc
    37801 gataaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg gtgcgaggat
    37861 gatgttgagg aaaattttat attctgctgg cgagtccacg ctctccccct ggactcgccg
    37921 cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt cactctttat
    37981 taagatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt accttcttct
    38041 gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc ggtgatgatc
    38101 tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt gtcattctca
    38161 accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc ggcacgttct
    38221 cgcgcttcat acggccccag acctaaactt tccagcgcca gacgcccggt tttcagcgca
    38281 ccttcaaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg aatgtagtga
    38341 tcgacatcgc gggcgcgggc aataatctgc aaatgcggga aatgttcttt caccatctcc
    38401 gtcagttgca ggttggtttg cggatcgtca atagcgttaa tcagcacttc cgctttcgcc
    38461 gctccggcag attccagtaa atccatccgc gtggcatcgc cataaaacac tttcatacca
    38521 aatttacgca gcgtttcgat atggtccgga tcgtgatcga gcaccaccat tttcaccccg
    38581 ctggagagca gtaaacgtcc ggtaatctgt ccaaaacgac cgaatccggc gataatcacg
    38641 cgcggctgtt cttcatcgat ctcatcggct tcacgcgcct cctccgtaga agattgctca
    38701 aggcgattga ggatcaccag cagaatcggc gttgctgcca tcgacagcgc caccgccagg
    38761 gtcagcgatt tcgcccactc cggctccagc acattcgcca tctgcgccgc accaaatacc
    38821 acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accagcgacg ctgtttattt
    38881 ggcacctgca acggtcgggc aatcagccac agcatggcga ttttgatgat gaggaaaccg
    38941 agcagcaaaa tgacaatgcg caatgggttt tcaatcagcg tgccaaagtc tatcgacatg
    39001 ccaacaccga tgaaaaacag ccccaacagc aaacctttaa acggttcgat atcgctttcc
    39061 aacgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc gcccatcgcc
    39121 atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacaaggaa taacgccacg
    39181 gcactaaaca cttcccgcaa gccagagcgg gcaacaaaac gcagcgccgg acgcgtaaca
    39241 tagcgcccca gcaataccac cagtaccagc gcacccgcca cttttaacgc cgagagagca
    39301 aatgcgccca tcgtcgtcga ggcactgctc gccgccagta gcgggatcat cgccaccagc
    39361 gggattgccg cgatatcctg gaacagcagc actgcaaagg cactgcggcc catttgcgtc
    39421 accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgttgatga gagcgccagc
    39481 gtcataccga tcaattccgc gacctgccag cgcagcccaa gtaacataca gaacagcccc
    39541 agcaggccac cgcaaatcac catctgcaat gcgccaccgc cgaacactgc cgcacgcagc
    39601 ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag caccacccca
    39661 atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc ccacgggcca
    39721 ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg taccgcaatg
    39781 ggtacaatca gcgccgccga tccgaggtag atcagcgcct gaaccagcgt atgactatcc
    39841 atgatgggcc tcctgccatt ccagcagacg ttgcttatag tggcgcgcct gcccttcgag
    39901 ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc agttcagccc
    39961 gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg gatgcgcacc
    40021 aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat gtttgccatg
    40081 cagcgccgtg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat cgatccaaag
    40141 tttaagaagc ggaggaatgc tgtaccactg catcgggtgc tgccagacga tcagatcggc
    40201 gcgagagagc gcctcctgct cggcggcaat atcgatattg aagtcaggat agagttgata
    40261 aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc gtttattcgc
    40321 atgggaatga tgcggatacg gatgcgcata aatgatgagg atcatagtta ttagcccgcg
    40381 tgtttacgcc tttgttttcc cacaaagtgt agtcatatat tcacgcggct tatagtcaat
    40441 aatattgatt cagaaaagag agaaatctga acaaataatc ccattctctg cccgcggatc
    40501 gtcatattgc tggtctgcgc cagtgacatt cctcgagtct ccggggcaaa cgctacggaa
    40561 atcaacaagc caaacagcga gatacccgcc cccattagca tcgtgttact gataccgtaa
    40621 ttattgataa atatcggcag tgcccaggtc gaaacaatgg tgccaatacg acttaaggac
    40681 ataatcacgc ccacggcaga ggcgcggata tccgtcggga agagttcatt aggatagagc
    40741 cactgcaaat tacccggccc gccagagaaa aaggcataca ccgcaaaggc catcactacc
    40801 agccagatcc ccatatccgg gattagcccc aaaaccgcca gcgccagcgt catcatggca
    40861 aagctgccaa tcaacaatgg acgccgtccg gcagtgttta gccacagcat cggcggaata
    40921 cagccgagca taaagaacag gctaatcacc acattcccta gtgccgcgtt tttgccaacc
    40981 cccaatccca acgaaccaac gatttgcggg ccaaaggtgt aaatggcgaa cattgggatc
    41041 acctggcagg tccagatggc ggcaacaaac agaacaaaag gaaaatggcg gcgattaaac
    41101 agatcgcgaa aacgggtttg ctgcggctgc tcttcatcga aagccaccgg ttcgccaaac
    41161 agttttatca tcatctcttc gcactctttt actcgccctt tgcgtaataa ccagcgggga
    41221 gattctggca gttcgaatcg accaatcaaa atcaacaaac aagggatcgc cgcgctaccc
    41281 agcatccagc gccaaccacc ttccacatca taaagccagt agccgaccag atcggcgcag
    41341 gtcgcgccga cataccacat cgcggcaata aagctgatgg aaaacgcccg ctgacgggta
    41401 ctggagaact cggtgatcat cgaagtggcg atgggataat ctgcaccgat gacaatgccg
    41461 atgagtaccc gcatcaccaa cagttcgacg ggggatgaaa cacacatcgt cgccaccgat
    41521 atcacgccga tggcgatgat atcaatgagg aacattttgc gccgtccgac tttatcggag
    41581 atatagccaa acagcgatgt gccaacgaac agcccggcga gcgttcccgc gcccagcaag
    41641 ccaatccagt cagcgtccag tttcagcgcc ggagtcagtt gctccagcgc cacgccaatc
    41701 attaccagta cataaccatc cagaaacgga ccaccgcttc cccacagcaa aatgcggcgg
    41761 tgaatagagg agaatttgag atcgtcaaag tttctggacg gttgcatggg ttattcctgt
    41821 ttttttgcaa tgagagtcag tacggcgtta cgtatcaaac caacatcagc cgtaacggaa
    41881 ctccacacca aaggtgccgc gcgggtattc ccactgttcc agcgccgacc ccagccccag
    41941 aatgcgacag gtgccgcact ccagacatcc ggcgtaatcg aagcgcacac tgccgtcatc
    42001 ctgcttcttg tacagacctg cggggcacgc tttcaccagc agctccagca cctgtttatc
    42061 agcatcggcc ttcacaacaa tgtgcggatg ctcttcatcg acattgaatt tattgacgcc
    42121 cagtttgacg tccacattga cgggagaagt cataaaacgg tcactccttt catgccatcc
    42181 ttgatcagat tgatgaagcc cactttcttg ccgtggcgga ggattttctt gcgcatcagt
    42241 tccggcgcac tgccgtcaat ggtgaacagg tcacgcgcca cgcccaccgc cagttccggg
    42301 tagccgctaa acatgcgtgg gttatcaagg aacgccggta gtttctggta catgcgcata
    42361 tcgcgcagcg ggctgctctc cagatgctga cgatattccg cgagtttttg cttactgaaa
    42421 tcgtcgcttt tcatcgctga aagcacggtt tttgctgcag cttccccggc ggcaatcgcc
    42481 agatccatac cgcgaatggt aaaaccgagg ttcatacaca ttccggcggc atcaccggca
    42541 atcaatacgc cgtcaccgac cagctccggc agcatgttga tgcctgcttc cggcactacg
    42601 tgagcggaat attccaccag cttgccgccc gcgatcagcg gtgcaacggc cggatgctgt
    42661 ttgaaatctt ccagcatttg cggcaccgat tttttcgcgt catgcagatg atgcagacca
    42721 caaaccagcc ccagcgacag ggtattttca ttggtataaa ggaagccgcc gcccatcagg
    42781 ccatcggtgg gtgatcccgc aaacagacaa gccgcgccct gattaccctg caactgaaaa
    42841 cggtcttcga ttaccgactt cggtgactcg atcagttcct tcacgccaac cgccacatcc
    42901 gtcggtttaa cgcgttttgc catccccaac ttttcggcaa ggatggagtt caccccatca
    42961 gcaaggagca ccgttttcgc ttcaatcaca tcgccatcgg cttctacacc gacgactttg
    43021 ccatcgcgct gtacgaggtt atcgacgcgg atcccggtaa ttaactgcgc gcccgcttct
    43081 tcggcctgct ccatcaacca ggcatcaaat ttactgcgca aaacggagta agaacgctgg
    43141 gatagcgagg tttcgtcacc gttgcagtag tccatagtca tcgctgactt ttccgtcata
    43201 aacgcgagtt tttcatgggt gatcaggcgt tctacggggg cgaaatcggc gaaacctgga
    43261 ataatgtgtt ccagactgtg ggcatagaga cgcccgccag taacgttctt ggcacccgcg
    43321 gaattgccac gctcgataac taacacttgc gccccttcgc gggcgagcac cagtgcggca
    43381 accgaaccgg caagccctgc accgacgatg atggcgtcaa agatatcttc ggacataaga
    43441 gctccctgtc agcggcagag ccgtaatgca gccctgccag agtggatcaa cgcgctaaag
    43501 ctgcggtcag tgccggaagg atcttcacgg catcgccaac aatgccgtaa tccgcgtact
    43561 ggaagatcgg cgcattttta tctttattga tggcgaaaat ggtttgcgac gcgttagcgc
    43621 caaccatgtg ctggatctgc ccggagatcc tcaccgccag gtacagttca ggtttcagca
    43681 tcaggttgga gataccgaca tagcgttcgt gctccatcca tttttcgttt tctgccaccg
    43741 gacgagaaca ggccaactcc gcacctatcg ccttgcaaag ctgttctgcc agtgcaatgt
    43801 tctctttgct gccaatgccg cgaccgacgc tgaccaccag acgggctttg tcgagatcga
    43861 cgctgttgct ctggcgcgtc tgggttgccg tgcgggtaat cgccacagcc ggagcctgcc
    43921 actccacggt gtgcgtttcg ccagtgcgtg acgcgtctgg ctgagccgca tcgaacgtgc
    43981 cgctgctgat ggtcagtacc gcatacggcg tggcaatgcg ttcttcgcca atcgccagac
    44041 cgccgtaaac catgtgtttc actgttgctt taccgtcctg tacgctgacg gtgctggcat
    44101 cgttagacac cgccgcttta aggcgataac ccagttttgc cgccagtaat ttgccgcgac
    44161 gggtgtttgg caacagcacc agaccgtcag cgccgtgctg gcgaatagtg tcagccatga
    44221 caccggcgta atcttcgatc atccgatcgt ccggtttgcc gtttaatttc cagacatgat
    44281 tagcgccgag ctggattgcc tgtgcgccgt cggcatcatt gaggacaaag gcattgattt
    44341 gattagctaa agcctgcgca ccgttcatca gttccggcag acgagagggg gtatcgctga
    44401 atacccagac ttgagaaaac gtgttcatag catcccctgt aattaaatga ctttgcgaag
    44461 attttcagca aatgcggcga tctgttcttc gccgtcgcct tcaatcacga tgcgctgacg
    44521 ctcgcgctgt ttcggcgcgg caacctgttg ttcagaccag gcgtccacgg cgttaaaacc
    44581 aatatccgcc gccgaccata cctggactgg ctttttcgcc gcgccgagaa tggctttcat
    44641 cgaaggaatt tgtggggagt tgatatcagt ggaaacagca acaaccgcag gcagcggaat
    44701 gcttaaggtt tcggtttcat cttccagttc gcgctcaacg gtgagggtat ctgccgtcag
    44761 ggagataatt ttgctgacgc cgttaactgc cggaatattg aggatttcgc ccaccagcag
    44821 accaacctgc tgggcataaa ggtcggaaga accatcgcca cagaggatca gatcaaagcc
    44881 tgctttctgg gcggctgcag ccagtgcgct cgccgtttgt tgcggcagtg cctgctcgaa
    44941 ctggtcatca atcaccacaa tcagttcatc cgggccgcgc gatagcacat ctttacgccc
    45001 tttggcgttg gtcagggctt taccgcccac acttaaggct gtcacctgcg cctctgctgc
    45061 ctgttgcttt agctggcaag ccgcttcaat agcgttgaga tcgtattggc ttattttggc
    45121 atcggctttg ctgaagtcta atgaaccatc agcattattg accgcaatat cctgttcatc
    45181 aggcacgcac ttatagcaag taataatctt cattgcatct ccagaaatca tgaaggaaac
    45241 gtcgttcata tcggcggtgt taattacaga tatcacgccc tttaatttgg aaataaaact
    45301 ttaatcacca atattgaaaa tgtcacacgc agataaatcc aactttcaat attgttaagt
    45361 tcctcaccaa tattgaaaac atggcgtagc aaaaagaaat tttcaatatt gttttataga
    45421 tcaccaatat tgaaagtcag atgctatttt ttacccacaa aagaacataa caaactgatt
    45481 aataaccaaa attaacgaaa aacatcctct attcactcga aacaaaaatg tgacaccaat
    45541 cacagaatac agcttattga atacccatta tgagttagcc attaacgcgt ccacgaggtt
    45601 aataataatt atattaaatg ttaacaaaaa taaaacaaac gggaacgcaa aataaatatt
    45661 cgttttcaca gtggaattaa ctcatgaaga atgaaaagag aaaaacggga atagaaccga
    45721 aggttttctt tccgccatta ataatcgtcg gcatactttg ttggcttaca gtcagagatc
    45781 tggatgcggc gaatgtcgtt attaatgctg tattcagtta cgtcaccaat gtatggggat
    45841 gggcatttga atggtatatg gtggtgatgc ttttcggttg gttctggctg gtgtttggcc
    45901 cgtatgccaa aaagcgttta ggtaacgaac cgccagaatt tagcaccgcc agttggatct
    45961 ttatgatgtt cgcctcctgt acgtctgctg ccgtactgtt ctggggatcg attgagatct
    46021 actactacat ctccaccccg ccgtttggct tagaaccgaa ctcgacagga gcgaaagagt
    46081 tggggctggc ttacagcttg ttccactggg gacctctgcc gtgggccact tacagcttcc
    46141 tttcagtcgc cttcgcttac ttcttctttg tccgcaaaat ggaagtgatt cgccccagct
    46201 cgacactggt gccgctggta ggtgaaaaac acgccaaagg gttgttcggt accatcgtcg
    46261 acaacttcta tctcgtcgcc ttgatcttcg cgatgggtac cagtctgggc cttgccacgc
    46321 cgctggtgac cgagtgtatg caatggttgt ttggcattcc gcataccctg caactggacg
    46381 ctatcatcat tacctgctgg attatcctca acgccatttg cgtcgcttgc ggtctgcaaa
    46441 aaggggtacg tatcgccagt gacgtgcgta gttacctgag cttcctgatg ctgggttggg
    46501 tgttcatcgt cagcggtgcc agcttcatca tgaactactt caccgattcg gtggggatgt
    46561 tgctgatgta tctgccgcgc atgttgttct ataccgatcc catcgctaaa ggcggcttcc
    46621 cgcagggctg gaccgtgttc tactgggcat ggtgggtgat ttacgccatc cagatgagta
    46681 tcttcctcgc ccgcatctcc cgtggtcgta ctgtgcgtga actgtgcttc ggcatggtga
    46741 tggggctgac agcatccacc tggatcctgt ggaccgtgct cggtagtaac actctgctgt
    46801 tgatggataa aaacatcatc aacattccaa atctgatcga acagtacggt gtggcgcgcg
    46861 ccatcatcga aacctgggcc gctctgccac tcagcaccgc caccatgtgg ggcttcttca
    46921 tcctctgctt tattgccacc gttacgctgg ttaacgcctg ctcttatacc ctggcgatgt
    46981 ctacttgccg cgaagtacgc gatggtgaag aaccaccgct gctggtgcgt atcggttggt
    47041 caattctggt tggcattatc ggtattgttc tgctggcgct cggcggcctg aaaccgattc
    47101 aaaccgccat tatcgccgga ggatgcccgc tgttcttcgt caacattatg gtgacgctct
    47161 cctttattaa agacgcgaaa cagaactgga aagattaatt aacccccaaa atatcaagag
    47221 gttgaaagat ggattttaat ttaaatgatg agcaggaact gtttgtcgcc ggtatccgcg
    47281 aactgatggc cagcgaaaac tgggaggcct attttgccga gtgcgaccgt gacagcgtct
    47341 acccggaacg ttttgtcaaa gcactggcgg atatgggtat cgacagtctg ctgatccctg
    47401 aagagcacgg tggtctggac gcggggtttg ttactctcgc cgccgtgtgg atggagctgg
    47461 gacgtctggg ggcaccaacc tatgtactgt accagctgcc gggcgggttc aataccttcc
    47521 tgcgcgaagg cacacaagag cagatcgaca agattatggc tttccgcggc accggtaagc
    47581 agatgtggaa ctcagcgatt accgaaccgg gcgcgggctc cgacgtgggt agcctgaaaa
    47641 cgacttatac ccgtagaaat ggtaagattt atcttaatgg tagtaagtgt tttattacca
    47701 gtagcgccta caccccgtac atcgtggtga tggcgcgcga cggggcttct ccggacaaac
    47761 ctgtctacac cgaatggttt gttgatatga gcaaaccggg catcaaagtg accaaacttg
    47821 agaagctcgg tctgcgtatg gatagctgct gtgaaatcac ctttgacgat gtggaactgg
    47881 acgagaaaga catgttcggt cgggaaggta acggctttaa ccgcgtcaaa gaagagttcg
    47941 accatgaacg tttcctggta gccctgacca actacggtac ggcgatgtgc gcctttgaag
    48001 atgcggcgcg ctacgccaac cagcgcgtgc agtttggcga ggctattggt cgtttccagt
    48061 tgattcagga aaaattcgcc cacatggcga tcaaattaaa ctccatgaaa aacatgctgt
    48121 atgaagcagc gtggaaagca gacaacggca ccatcacctc tggcgatgca gcgatgtgca
    48181 aatacttctg cgccaatgcg gcatttgaag tggtggatag cgcaatgcag gtgctgggcg
    48241 gtgtcgggat tgcgggcaac caccgcatca gccgcttctg gcgtgacctg cgtgtagacc
    48301 gcgtttccgg aggatctgac gaaatgcaga tcctgacgct gggtcgtgcg gtgctgaagc
    48361 aataccgcta agtccgtgcg ctgcctgatg cgacgctgac gcgtcttatc aggcctacgg
    48421 gagcacgaat gtaagccgga taaggcgttt acgccgcatc cggcagtcat gcgctacata
    48481 aatttttcag gagctaataa tggatcatct acccatgccg aaattcggac cgctggccgg
    48541 attgcgcgtt gtcttctccg gtatcgaaat cgccggaccg tttgccggac aaatgttcgc
    48601 agaatggggc gcggaagtta tttggatcga gaacgtcgcc tgggccgaca ccattcgcgt
    48661 tcaaccgaac tacccgcagc tctcccgccg caatttgcac gcgctgtcgt taaatatttt
    48721 caaagatgaa ggccgcgaag cgtttctgaa attaatggaa accaccgata tcttcatcga
    48781 agccagtaaa ggtccggcct ttgcccgtcg tggcattacc gatgaagtac tgtggcagca
    48841 caacccgaaa ctggttatcg ctcacctgtc cggttttggt cagtacggca ccgaggagta
    48901 caccaatctt ccggcctata acaccatcgc ccaggccttc agtggttacc tgattcagaa
    48961 cggtgatgtt gaccagccaa tgcctgcctt cccgtatacc gccgattact tttctggcct
    49021 gaccgccacc acggcggcgc tggcagcact gcataaagtg cgtgaaaccg gtaaaggcga
    49081 aagtatcgac atcgccatgt atgaagtgat gctgcgtatg ggccagtact tcatgatgga
    49141 ttacttcaac ggcggcgaaa tgtgcccgcg catgagcaaa ggtaaagatc cctactacgc
    49201 cggttgcggt ctgtataaat gtgccgacgg ctacatcgtg atggaactgg tgggcattac
    49261 ccaaattgaa gagtgcttta aagatattgg cctcgcacat ctactcggta cgccggaaat
    49321 cccggaaggc actcagctta tccaccgtat cgaatgccct tacggcccac tggtagaaga
    49381 aaaactcgat gcctggctgg cggcacatac catcgcagaa gttaaagaac gctttgccga
    49441 actgaatatc gcctgtgcca aagtactgac cgtaccggaa ctggaaagca atccacagta
    49501 tgttgcccgt gaatcaatca ctcagtggca aacgatggat ggtcgcacct gcaaagggcc
    49561 gaacatcatg ccgaaattca aaaataaccc cggacaaatc tggcgcggaa tgccctcaca
    49621 tggcatggac acggctgcca ttttgaaaaa tatcggctac agcgaaaacg acattcagga
    49681 gttggtcagc aaaggtctgg ccaaagttga ggactaaacg cgtggcgtca gtcggcttgc
    49741 ctgactagcg cccgcccaat aacaaacgaa caaaaatgga tagaggtgca atggatatca
    49801 ttggcggaca acatctacgt caaatgtggg acgatctggc ggacgtttac ggtcataaaa
    49861 cggcgctgat ttgtgaatcc agcggcggag tcgttaaccg gtatagttat cttgagttaa
    49921 atcaggagat taaccgcacg gcaaacctgt tttatacgct ggggattcgc aaaggcaaca
    49981 aggttgcact acatctcgac aactgcccgg aatttatctt ttgctggttc gggctggcaa
    50041 aaattggcgc gattatggtg ccgattaacg cccgcctgtt acgcgaggaa agcgagtgga
    50101 tcttgcaaaa tagccaggcg tgcctgctgg tgaccagtgc gcaattttat cctatgtatc
    50161 aacagattca gcaggaagat gccactcaat tgcggcacat ttgcctgaca gatgtggcac
    50221 ttcccgctga tgatggagtg agttcattta ctcaactgaa aaatcaacaa cctgccacct
    50281 tgtgctatgc accgccgcta tcgactgacg atacggcgga aattctcttc acctccggca
    50341 ccacctcccg accgaaaggt gtggtgatta cccattacaa cctgcgcttc gctggatatt
    50401 actccgcctg gcagtgtgca ctgcgtgacg atgacgtcta catgacggta atgcctgcgt
    50461 ttcatatcga ttgccagtgt actgcggcga tggcggcgtt ttctgccggg gccacctttg
    50521 tgctggtcga gaaatacagc gcccgcgcct tctggggaca ggcgcagaag taccgcgcca
    50581 ccattaccga atgtattccg atgatgatcc gtacgttgat ggtgcagccg ccttcagcga
    50641 acgatcggca acaccgcctg cgggaagtga tgttttatct caacttgtcg gagcaggaaa
    50701 aagacgcatt ttgtgaacgc ttcggcgttc gcttgctgac gtcttatggg atgacggaaa
    50761 ccattgtggg cattatcggc gatcgcccta gcgataaacg acgctggccg tcgattggtc
    50821 gggcggggtt ttgctacgaa gcggagatcc gcgacgatca caatcgcccg ctcccggcag
    50881 gtgagatcgg tgaaatctgt attaaaggcg taccagggaa aaccatcttc aaagagtatt
    50941 ttctcaaccc gaaagccact gcgaaagtgc tggaagccga cggctggcta cataccggcg
    51001 ataccggata ccgcgacgaa gaaggctttt tttatttcgt cgatcgccgc tgcaatatga
    51061 tcaaacgcgg tggcgagaat gtctcctgcg tggagctgga aaatatcatc gccacccatc
    51121 cgaaaattca ggacatcgtg gttgtgggta ttaaagattc gattcgcgat gaagccatca
    51181 aagcatttgt ggtgctgaat gaaggtgaaa cattgagcga agaggaattt tttcgcttct
    51241 gcgaacaaaa tatggcgaaa tttaaagtgc cctcttatct ggagatcaga aaagatctgc
    51301 cacgtaattg ctcggggaaa ataattagaa agaatctgaa ataaatgaaa cgtcagggaa
    51361 ctaccctgcc agctaataat cacgccctaa aacaatatgc attttttgca gggatgttgt
    51421 cgtccctaaa aaagcaaaaa tggagaaaag gaatgagtga atcattacat ctgacccgca
    51481 atggatcaat tctggaaatt acccttgatc gcccaaaagc gaatgctatt gatgcaaaaa
    51541 ccagctttga aatgggcgaa gtatttctaa atttccgtga cgatccgcaa ttacgtgtcg
    51601 ccattattac cggtgccgga gagaaattct tttccgcggg ctgggattta aaagcggcag
    51661 cagaaggcga agcaccggat gctgactttg gcccgggtgg ttttgcggga ttaaccgaaa
    51721 ttttcaatct cgacaaacca gttatcgcag ctgtgaacgg ctacgccttt ggcggcggtt
    51781 tcgaactggc gctggcggca gattttattg tttgtgccga taacgccagc ttcgccctgc
    51841 cggaagccaa actgggcatc gttcctgaca gcggcggtgt gctgcgtctg ccgaagatcc
    51901 tgccgcctac catcgtcaat gaaatggtga tgaccggcag acgaatgggc gcagaagagg
    51961 cgctgcgttg ggggatagtc aaccgcgtgg ttagccaggc ggaactgatg gataacgccc
    52021 gcgaactggc tcagcagctg gttaacagcg ccccgctggc gattgcggcg ctgaaagaga
    52081 tctaccgcac caccagcgaa atgccggtag aagaagcgta tcgctatatt cgcagcggcg
    52141 tgttgaaaca ctatccatcg gttctgcatt cggaagatgc cattgaaggg ccgctggcgt
    52201 ttgccgagaa gcgcgatccg gtgtggaaag gacgttaaca accgtgagct attacgcctt
    52261 tgaggggtta attccggtgg ttcacccgac ggcgtttgtc catcccagtg ccgtcttgat
    52321 tggcgatgtg attgtgggag ccggtgtcta catcggccca ctcgcctcac tgcgtggtga
    52381 ctacgggcgg ttgatcgtgc aagcgggagc caatattcag gatggctgca ttatgcatgg
    52441 ctactgcgac actgacacca tcgttgggga aaacggccat atcgggcatg gagcgatcct
    52501 gcatggttgt gtgattggtc gcgatgcatt ggtcgggatg aacagcgtaa ttatggatgg
    52561 cgcggtcatt ggcgaagaga gcattgttgc cgcaatgagc tttgtcaaag caggctttag
    52621 tggcgagaaa cgccagttgt tgatgggtac gccagcccgc gctgtacgca gtgttagtga
    52681 cgacgagtta cactggaaac ggttgaatac caaagagtat caggatcttg tcgggcgctg
    52741 tcatgcatcg ttacatgaaa cgcagccact gaggcaaatg gaggaaaatc gcccccgttt
    52801 gcaggggacg acggatgtga cgcctaaacg gtgatcttgc cggatgcgac ctggttgctg
    52861 gataagatgc tacaacatcg tatctggcaa gcaggacgcc cggtatcaac gagataaaat
    52921 taacgacgca tactctttga caacatcatc tgccacttct gctcgcggtt aagttccgtg
    52981 agcggaacgg caggaatacg cggtgtcttt cttacgccga ctaatttttc ccgactgtta
    53041 ttgcgcaggc gcgcgtaaat ttgctcatcg atatcgacca ctttaaccag tcgctgacac
    53101 tggcatcccc gcccttccag cttattaggg atcatcttaa cttcgcagct tatttctgtg
    53161 acttccgaca gaatataagt cagggtatta accgccttgc tgtgttcaat atcgaaatga
    53221 atagagatct ctcttgctat cacccaccga ttttcagcca tcatccattc ggcgattaac
    53281 aagtagagtg gtttttcaac atatccttca cacatacgaa ctccagtttc acaaattaaa
    53341 agtctggcta tgactcgttt tgaagcaatt aaacaagacc atattaaaat tgtggatata
    53401 tcgatagtat gcaacttcac cgtcgataag tgtgagctaa atcctgctta tgtcatcaaa
    53461 aatatcgatt ccccaaaaga tttacttaat gggcaaaaaa aaacggtcct catcagagaa
    53521 ccgtattagt tattcgatca aattagcgga tgaaaaatat ccgccacgac acgctattat
    53581 ttgatctgtg cgtgcatttc ctgcaccgaa attacttttt cagtcgcatc ggcattcagc
    53641 gccatcgcgg tagcgaaacc accgttcagg gtggtgtcat aatgcacttt atattgcagc
    53701 gcactgcgac ggatcacgcg ggagtcttca atcgcacgac ggcctgaggt ggtgttgatg
    53761 atgtaggtat attcgccatt cttgatacgg tcctgaatgt gcggacggcc ttcatgcacc
    53821 ttgtttacca gacgcggatt gatacccgct tcgcccagca caatcgccgt gccgtgggtc
    53881 gcatccagct cgaagccctg tttcagcagt tttgccgcca ggtccaccac gcgttcttta
    53941 tcgccttcgc gcacggaaag cagcgcacga ccgtgtttct tcatggtgga gttgctgccc
    54001 agctgcgctt tggcgaacgc ttcagcgaag gtgcggccca cgcccatgac ttccccggta
    54061 gagcgcattt ctggccctaa cagcgggtca acgcctggga atttattgaa cggcagcacc
    54121 acttctttca ccgagtagta cggcggaata acttctttgg ttacgccctg ctcagccagc
    54181 gatttgccag ccatcacacg cgccgccact tttgccagcg gtacgccggt ggctttggag
    54241 acgaacggaa cagtacgcgc cgcacgcggg tttacttcaa tcaggtaaac ttcgttgttt
    54301 ttcaccgcaa actgcacgtt catcagaccg cgtacctgca attcgaaggc cagtttctgc
    54361 acctgctggc gcatcacatc ctgaatttcc tgacttaagg tgtacgctgg cagcgaacat
    54421 gcggagtcac cggagtgcac gcccgcctgc tcgatgtgct ccatgatgcc gccaatcagc
    54481 accatttcgc cgtcgcagat ggcatccacg tcaacttcta ctgcgtcatc aaggaaatga
    54541 tccagcaaca ctggcgcatc gttagacacg ctaaccgccg tctggaagta gcgacgcagg
    54601 tcagcttcgt catagacgat ttccatcgcc cgaccgccga gaacgtaaga cggacgcacc
    54661 accagcgggt agccaatctc ttttgccttc tcaactgcca tttcaatagc agtaacggtg
    54721 gcgttcgccg gttgtttcag tttcagacgg tcaaccgcat gctggaagcg ttcacggtct
    54781 tccgcacggt cgatagcatc cgggctggtg ccgataaccg gtacgccagc ggcttccagt
    54841 gcacgcgcca gtttcagcgg ggtttgaccg ccgtactgaa cgataacgcc tttcggcttc
    54901 tcaatacgca cgatttccag cacatcttcc agagttaccg gctcgaagta gaggcgatcg
    54961 gaagtgtcgt agtcggtaga gacggtttcc gggttacagt taaccataat ggtttcgtaa
    55021 ccgtcttcac gcagcgccag cgaggcgtgt acgcagcagt agtcgaattc gataccctga
    55081 ccgatacggt ttggaccgcc gccaagcacc atgatttttt cacggtcggt agacggattc
    55141 gcttcgcact cttcttcata agtggagtac atgtaagcgg tgtcggtggc aaactctgcc
    55201 gcacaggtat ccacgcgctt gtagaccggg tgcaggtcat attggtcacg cagcttacgg
    55261 atttccgctt cgcgtacgcc cgccagtttt gccaagcgcg catcggcaaa gcctttgcgt
    55321 ttcagctggc gcaggaattc agcgttcagg ccagtgatgc ccacttccgc cactttctct
    55381 tccagacgca ccagttcttc aatctgtacc aggaaccagc ggtcaatgtt agtcaggttg
    55441 aagacgccgt ccacagacag gcccgcgcgg aaagcatcgg cgatgtacca gatacgctct
    55501 gcgcctgcgt ctttcagttc gcgacggatt ttggttaacg cttccgggtc atccaggctc
    55561 actttcgggt cgaagccagt tgcgcccact tccaggccgc gcagcgcttt ttgcagggat
    55621 tcctgctgtg tgcgaccaat cgccatcact tcgccaaccg atttcatctg agtggtcaga
    55681 cggtcgttag caccggcgaa tttttcgaag ttgaagcgag gaattttagt aaccacatag
    55741 tcgatggacg gctcgaagga ggccggagta cgtccgccag tgatgtcgtt catcagttcg
    55801 tctagggtgt aacccaccgc cagtttcgcc gccactttag caatcgggaa accggtcgct
    55861 ttcgacgcca gcgccgaaga acgggacacg cgtgggttca tttcgataac aatcaggcga
    55921 ccgtttttcg ggttcaccgc aaactggaca ttggaaccac cggtttcaac gccgatttca
    55981 cgcagcaccg ccatcgaggc gttacgcatg atttgatatt ctttgtcagt cagcgtttgg
    56041 gctggcgcga cagtgatgga gtcaccggtg tggatgccca tcgcatcaaa gttttcgata
    56101 gagcagacga tgatgcagtt gtcgttttta tcacgcacca cttccatctc gtactctttc
    56161 cagccgatca gcgactcatc aatcagcaac tctttggtcg gagagagatc cagaccgcgg
    56221 gcgcaaattt cttcaaactc ttcacggtta taagcgatac cgccgccgct accgcccatg
    56281 gtaaaggatg ggcgaataat gcacgggaag cccacgtcag cggcaacagc cagcgcttct
    56341 tccatcgtgt gtgcgatacc ggaacgcgcg gtttccagac caattttctt catcgctacg
    56401 tcgaaacgac ggcggtcttc tgctttatca atcgcatcgg cagtggcacc aatcatggtg
    56461 acaccgaact cttccaacac gccctgacgt tccagctcca gcgcgcagtt cagcgccgtc
    56521 tgaccgccca tggttggcag caccgcgtcc gggcgctctt tttcaataat cttgcgtacc
    56581 acttcccagt gaatcggctc gatgtaggtc gcatcggcca tttccgggtc ggtcatgatg
    56641 gtcgccgggt tggagttcac cagaatgacg cggtaaccct cttcgcgcag ggctttacat
    56701 gcttgcgcgc cagagtagtc aaactcacac gcctgaccga taacaatcgg gcccgcaccc
    56761 agaatcagga tactttttat atctgtacgt tttggcatgg ctcttttact cctgattact
    56821 tagcggtttt acggtactgc tcaattaact tgataaagtg gtcgaacaac ggcgcggcgt
    56881 cgtgtggacc agggctggct tcagggtgac cctggaagct gaacgccggt ttatcgatac
    56941 gatgaatgcc ctgtaacgta ccgtcgaaca gggatttatg cgtgacacgc aggtttgcag
    57001 gtaatgttgc ttcatccacc gcaaaaccgt ggttctgggc ggtgatcatc accacgtttt
    57061 tctccacatc tttaaccgga tggttgccgc cgtggtgacc aaatttcatt ttgacagtct
    57121 tcgcaccgct cgccagcgcc agcagctgat gaccgagaca gatgccgaat accggaatat
    57181 cggtttcgag gaacttctgg atggcggtaa tggcgtaatc gcacggggcc gggtcgccag
    57241 gaccgttgga gaggaagatg ccgtctggat tcattttcag cacatcttcc gcagaagttt
    57301 gcgccggaac gatggtcagg cgacagcctc tatccaccag catccgcagg atgttgcgct
    57361 tggcaccaaa atcataagcc acgacgtgga acggcagctc gtcttctttt ttcgcttctg
    57421 gcaggccgcc ggtcaacgtc cagctccctt gtgtccagct ataggcttct gcggtggtca
    57481 cttcttttgc cagatccatg ccgttcagac ctgggaacgc gcgggctttt tctaacgcca
    57541 gcgccgcatc cgggttatcg cctgcgataa tgcagccatt ctgtgcgcct ttctcgcgca
    57601 gtaaacgcgt cagcttacgg gtatcgatat cggcaatcgc cacgatgtta tggcgcttca
    57661 ggtaagaaga gaggtcttcg gtattacgga agttgctggc aatcagcggc aggtcgcgaa
    57721 tcaccagacc ttgtgcatgt acctgagaag attcttcatc ggcgtcatta gtgccgacat
    57781 tgccaatatg gggataagta agagtaacga tttgacgaga ataggaagga tcagtgagga
    57841 tttcttgata accggtcatt gaagtattga aaacgacttc cccaaccgcc gaacctgttg
    57901 cccctatggc ccgaccgtga aactgggttc cgtcttccag aaccaatagc gctgacttaa
    57961 tcaaaacacc ctccatagaa tattcactca ctttatttgc atattaattc acagtgatga
    58021 cgtaaatcaa tgcaaatctg cttaccgacg aatttctggc aaacggcggc attctggaga
    58081 tatgggcgct aaaagtcaac ttaatggcag gattttttat attattttgt ggctcatgca
    58141 tgttttacag gcattaagcg acaaaaacat catctaacct gttctggaaa acgcttgcgc
    58201 agcaagaaga ttgcatgaaa taaaaacgag caggaaaaag tggaccaaat ggtcaaaatt
    58261 tacattaaaa caacaaaaat agaagacgaa acatgtaatt tttggcagtt atagaaaatt
    58321 taaaagcata acaccaaaaa taaaagggct aaaaattaaa gccctttata attaataaat
    58381 taaatgtgtt acttttgcat cataataaac attttgtggt tacaaattat tgagatcaag
    58441 cacatctcgc atatcaaaaa gaccactttc cttaccactc aaccacaaag ccgatcttac
    58501 cgcaccgtta gcaaatgtca tgcggctgga cgccttatgg gtgatctcca gacgctcgcc
    58561 aatatcggca aacatcgcgg tatgttcgcc aacgatgtca cctgcacgca cggtggcaaa
    58621 accaatggta ccaggcacac gttcaccggt atggccttca cgactgtaga ccgcgcaatc
    58681 tttcagatct ttatcaaggg cgtgggcgat cgcctctccc attgccagtg cggtgcctga
    58741 cggcgcatca actttatgtc tatgatgtgc ttcaataatt tcgatatcgg tgtagtcacc
    58801 catcactttg gctgctttct ccagcagctt aagcatgacg ttaacgccaa cgctaaaatt
    58861 ggcggcaaag acaatcgcaa tatcggcggc ggcgtcacga attgcctgtt taccggcttc
    58921 gtcaaacccc gtagtgccga tcaccatccc tttgccatgc tgccgacaaa aagcgagatg
    58981 gttcagcgta ccttccggac gggtaaaatc gataaacaca tcaaaatcat cttttatcgc
    59041 atcaagactg ctttgcacgg taacgcctgt tttccctgca ccagccagct caccggcgtc
    59101 gctgcccagt aaagaagatc cttcgcgctc cagcgccgcg cccagctgca cgccttctaa
    59161 tgccagtgcc gcctgaatca actggcggcc catacgcccc ccggctcccg cgatggcaac
    59221 gcggatgttt gcatcatgca tagctattct cttttgttaa tttgcataga ccgttttcag
    59281 agtaaccagc gcatcgctac gccgccagcc aaaaacgctg ataattagaa attaatgata
    59341 aacagggagg gatatcagtg aaaggcatga gtcgatgaat gactgcatgc cgataacatg
    59401 tgacaggtta cggcaccaga gccagcacct cagccaccca ctgttggaag ccttttacat
    59461 tcagatccaa tgccacctgt acattggctg gtttgcccag gcaaccgtcg atatcaacca
    59521 ccgtcgtgcc tgaggtaaat tcgccttgag tttctaccgc gacaaaacac ggcttgaggg
    59581 tgaataagtc cgggcgcacc agccaggcga tggcgcagag atcgtgcatt cgcagaccgc
    59641 tttgcatact gccgctacgg tagtggctaa acagggcgtg gagcattttt ccggtgcgat
    59701 tcaactccgg taaagtagcg agatagtcag gggttaatat tgcctgattg gtgacatcca
    59761 gaccgcacat gacaatttcg atgccgctac ggaagacaca ggcagccgct tctggatcgg
    59821 cagcgatatt aaactcggca tttggtgtac agttgccgcg accagcagaa ccgcccatga
    59881 tcaccagacg acgaatatac ggtttacatt ccgggcattg taaaagtaac agcgcaatat
    59941 tggttaacgg gccgatggcc accagggtaa cgggctctgg tgcacgcatc agggcatccc
    60001 gaatcgccag aaacgccggt atcccgagtg gctttcggtt gtgctcaaca aagtcgtagc
    60061 cagccattcc cgattcgccg tgcacagatg ctgcatcacg cggtgcgcgt accagcggca
    60121 cagcggctcc ctgggcgagc ggaatctccg cattccagaa atgcagcagt tgcagggcat
    60181 tgcgggtagt tttctcaacc gagacattac ccgcgacggt ggtcatcagt tgcaggtcga
    60241 gttcgggggc aaaaatcgcg gcggcaatgg cgacggcatc gtcaatgccg gggtcagtat
    60301 cgaggaatat aggtaaacgc atgttttctt cataaaaaat gccggtaaca agaccggcat
    60361 tttcgcataa cttaggctgc taatgactta atcgacttca cgaatatcga cacgcagctc
    60421 tttcggcact tcgaaaacaa tattttcttc acggccttcc agcggaatgg cttcaccacc
    60481 acccaactgc tgcaaacgtg ccaccacatt ctgcaccaga atatccggag ccgatgcgcc
    60541 cgcagtcacg ccgacgcatt taacctcttt cacccactct tcctggatat ctttcgcatc
    60601 gtcaatcaaa aacgcgtgtt tgcccatacg ctgggccagc tccgccagac ggttggagtt
    60661 ggaggagttt ttcgaaccga ccaccaacac aacttccgcc tgttctgcca gggcgcgtac
    60721 cgcttcctga cggttagtcg tggcgtagca gatgtcatct ttgcgcggac cgacaatttt
    60781 cgggaagcgt ttacgcagcg cgtcgatcac atcagacgta tcatccaccg acagcgtggt
    60841 ttgggtcata aaggagagct tctcttcgtt tttgaccgtc agtttccaca catcgtctgg
    60901 cgattcgacc agatacattc ccccttcggg gttgctgtac tgccccatcg tcccttccac
    60961 ttccgggtgc ccggcgtggc cgatgagaat agattcttcg ccacggcgac tggcgcgggc
    61021 aacttccata tgcactttgg tcaccagcgg acaggtggca tcaaacaccg tcaaatcgcg
    61081 gcttttcgct tcgttacgaa ccgcctgaga aacaccgtgt gcggagaaaa tcaggatcgc
    61141 gccgtccggc acttcgctaa tctgctcaat aaagatagcc ccacgctcgc gcaagctatc
    61201 gaccacgtag cggttatgca ccacttcgtg acggacatat atcggtgcgc cgtaaatggc
    61261 cagcgcgttt tcaacaatgc tgatagcgcg gtctaccccg gcacaaaaac cacgcgggtt
    61321 ggccaacagg atctgcatgt tacgcctcca gtgccggatc gatttccagc acttcaatat
    61381 caaaatgaac ggtctgcccg gccagcggat ggttgaaatc aacggtaatg gagtcgccgt
    61441 taatttcgcg gatcacacca ggcatctcac tgccatccat tgcggtaaaa agcatgattg
    61501 cgccaatttc tggctcgcct gcatccataa attcacggcg ggagaagtac tgaatcaggt
    61561 ccggtgacgg cacgccaaac gccgcatctg gctccagcga gaaggtggtt ttgtcgccca
    61621 ctttcagccc cagcaggtgt tgctccagcc cttcagaaag agaagcatcg cccaggcgga
    61681 acagcgccgg tttaccgttg ttgcgagtag actcggcggt ggtgccatcg tcaagtttta
    61741 gcgtgaagtg caccaggacg gcgctattgc tctgtacaga ttcagacatg caggtttgct
    61801 cttatttttg aggatttttg ccggatgcgg cctacgattt cgtagcaatc tgtaggccgc
    61861 atccggcagg gtttattatt gttttttcgc tttggaaggt aaaaaccctt ccagcacgat
    61921 cagtgccgca ccgacacaaa tggcagtatc ggcaaggttg aaggtggcga agtgccagtc
    61981 gccgacgtag aagtcgatca tatcgacaac gaagccgtgc cacaggcggt cgaacaggtt
    62041 gcccagcgcg ccgccaataa tcagcgcgta agcgatattg tttagcttct gcgtggcctt
    62101 cgagcgatac atcatcactg ccaggagcac gctaatacca atcgcaatac cggcaaagaa
    62161 ccaacgctgc cagccgccgc tatcggcaag gaaactaaac gccgcgccat agttacgcgc
    62221 ataatgcaga ttaagcgacg ggaacagcgg gaccgtatcc cccagagcaa agttctggag
    62281 gatcaggtat ttgctgccca gatcgataat cagcacgact accaccagcc acagccagcg
    62341 tagccctgtt gaacagatcg attgactcat caggcaaact tacgtttttc accgtcaccg
    62401 gcgacgttgc tgacacagcg gccgcagatt tctgcgtgtt ccgccacctt gccgacatcc
    62461 tgggtgtagt gccagcagcg tgggcacttc tcaccttcgg ctttactcaa cgcgactttc
    62521 agccctttga gtacttcgct ctgctgagca tcagcaggtg cgtcgttata gtctgcaacg
    62581 gtagcgccgg aggttaacag gacaaatcgt aattcatcgc ccagcgcggt cagtttcgcc
    62641 gccagttccg gttctgcata caaggttact gccgcttcca gcgagccacc cactttcttg
    62701 tcggcacgcg cttgctcaat gactttgttc acttcgccac gcactttcaa cagctcgtcc
    62761 cagaacgcat cgttcattgc ttcactgtct gccagaccaa acaggccttc gtaccactca
    62821 ccggtgaaga cgtatttttc acgttcgccc ggcagatagc cccacacttc atcagcggtg
    62881 aaggagagga ttggtgccat ccagcgcacc agcgcttcag cgatgtgata cagcgcagtc
    62941 tggcagctac gacgcgccac gctatccgct ttggcggtgt actgacggtc tttgatgatg
    63001 tcgaggtaga aggaacccat ctcaacggag cagaagcgca tcagacgctg taccacttcg
    63061 tggaaatcgt atgcttcgta cgccttgagg atgtcttcct gtgccgcttt cgcacaacct
    63121 acggcccagc gatccagtac caccatctct tccggtttca ccatatcttt tgctggatca
    63181 aaaccattca ggtttgccag caggaagcgc gcggtgttac ggatacgacg atagctatcg
    63241 gcagcacgtt tcaggatctc gtcagaaacg gccatttcac cggtgtagtc ggttgatgcc
    63301 acccacagac gcagaatatc cgcgcccagt ttgttcatca catcctgcgg cgaaacggtg
    63361 ttgccgatgg atttagacat cttgcgaccc tgaccatcca cggtaaagcc gtgggtcagt
    63421 acctgacgat acggcgcttt gcctttcatc gcggtggaga tcattaggga agacatgaac
    63481 caaccacggt gctggtcaga accttccaga tacatgtccg ctgcgtgacc ggcaaattcc
    63541 ggacgcacgt caacaacaga agagtgggta gatccggagt caaaccatac gtcgagagta
    63601 tccggcactt tcacatactg gtcagcttcg tcgccgagga tctctttcgc atcgagatcc
    63661 caccacgcct ggatgccgtc aacttcaacg cgtttagcca cttcttccat cagttcgagg
    63721 gtacgcggat gcagctcttc cgtgtctttg tgcacgaaca gtgacatcgg tacgccccag
    63781 gtgcgctgac gggagataca ccagtcagga cggttagcaa ccatcgactc gatacgcgcc
    63841 tggccccagt ccgggatcca ctgcacgcct ttgatctctt tcagtgactg cgcacgcaga
    63901 cctttctgat ccatgctgac gaaccactgc ggtgtcgcgc ggaagatgat cggcgttttg
    63961 tgacgccagc agcacggata gctgtgctgc attttctcaa cgtgcagcag cgcgcctttt
    64021 tcctgcagca gcgcaacgac gatgtcgttc gctttgaaga cgttcacgcc atccagcgtc
    64081 ggataagtgc ccggcagata agtgccgtcc gggccaaccg ggttagcggt ttccaggccg
    64141 tatttctgac cgatcacata gtcgtccggg ccgtggccag gcgcggtgtg aacggcaccg
    64201 gtaccggcat ccagggtaac gtgatcgccg aggattgccg gaacgtcgaa gcccataaac
    64261 ggatgggtaa agcgcagcag ctcaagctct gcacctttta ccgtgccgag aatggtgtaa
    64321 tcggtcacgc cgatacgctg cattacgctt tcaaccagat ctttcgccag aatcgcggcc
    64381 tgaccgtcga tctgcaccag cgcatagtcg aaatcaggtg caatagagat tgcgcggttc
    64441 gcaggcagag tccacggcgt agtggtccag atcaccagcg agattgggcc gttaacgttg
    64501 cttacgccaa atttcgcttt cagcgcatcc tgatcgaccg cctggaaagc gacgtcaatg
    64561 gacggagaag ttttgtcgta atactcaact tccgcttctg ccagtgcaga acggcagtct
    64621 acgcaccagt gcaccggctt cgcgcctttg tgcaggtgac cattgccgat gattttgccc
    64681 agcgcgcgga tgatgttggc ttcagttttg aagtccatag tcagatacgg gtgcgaccag
    64741 tcgcccagca cgcccagacg gataaagtct ttgcgttgac cgtcaacctg ggtcgccgcg
    64801 tattcgcggc acttggcgcg gaactcggcg gcggtgaatt tctcacccgg cttaccgtat
    64861 tcttgctcta ctttcagctc gatcggcaga ccgtggcagt cccagccagg cacatacggc
    64921 gagtcataac cggaaagccc tttggacttc acgataatgt ctttcagaat cttgttaacc
    64981 gagtgaccaa tatgaatgct gccattcgca taaggagggc catcatgcag aatgaaggtt
    65041 tttttgcctt ttttagccgc acggatgatg ccgtacagat catcatcagt ccaacgcgcc
    65101 agcattcccg gttcgcgctt ggcgagatcg ccacgcatcg ggaaccctgt ttccggcaaa
    65161 ttcagggttg atttatagtc actcatcaga ttctcggttc cgtgtttcgg tttgattaca
    65221 taacaggctt aagccggttt tgttagccca aaaaattcgc gggcggttaa ttcatcacgc
    65281 gcaatctgcg ctttcagttc gtccagcgac gcaaatcgct gctcattgcg tattttttta
    65341 cgcagcacta cttgtatatg gcgaccgtaa aggtccattg caacatctaa caaatgcact
    65401 tccagctgct ggcgaatacc ggcaaccgtt gggcgtgttc cgatgtttgc cacgccgggt
    65461 aacggctttt caccgaggcc cagcacttct accgcataaa cccctttcac cggggaaacc
    65521 tgacggcgta gcggtacatt cgccgtcggg aaacctatag tgcgccctaa ttcatcaccg
    65581 tggactacac gcccggagat ggcaaacggg tgccccagta aactttctgc cagagccaga
    65641 ttgtcatcag ccagcgcctg acgcacggcg gtgctgctga tacgcacacc accttcgcaa
    65701 aaagtttgcg tactggtgat atcgaagccg tattccatgc cagctttctg taataacaag
    65761 aaatcgcctt cacgaccagc gccaaagcgg aaatcatcac ctacggcaag aaattttacg
    65821 cgcaaatgct tcaccaaaag atcgctgatg aaattttgcg cggttaacgc cgcaaaacgc
    65881 ctgtcgaaac gcacgcacag cacgtaatca acgccacact ctgcaaggta acgcagtttt
    65941 tcccgcagcc gggtcagtct tgccggggct ttatcggtag caaacagttc cagtggttga
    66001 ggttcaaaaa gcatcaccat caccggtaag ttgcgcttgc gcccttcttc ctgcaagccc
    66061 tgtaacagcg cgcgatgacc gcgatgcacg ccgtcgaaat taccaatagt cagcacacac
    66121 ccttcttgcg gggcctggct gagattatgt atgccgcgta tcagcttcat gtctggctca
    66181 aaacagtgaa aatcgtccga gtataccttg tacagcggtc aaggttaacc ggcgattgag
    66241 taccgaaatc agaaagaagt ggcttttcat tgccatggcg caaatcacgg gaagaaactg
    66301 accgcctgct gcaattttta tcgcggaaaa gctgtattca caccccgcaa gctggtagaa
    66361 tcctgcgcca tcactacgta acgagtgccg gcacattaac ggcgcttatt tgcacaaatc
    66421 cattgacaaa agaaggctaa aagggcatat tcctcggcct ttgaattgtc catatagaac
    66481 acatttggga gttggacctt ggctaatatc aaatcagcta agaagcgcgc cattcagtct
    66541 gaaaaggctc gtaagcacaa cgcaagccgt cgctctatga tgcgtacttt catcaagaaa
    66601 gtatacgcag ctatcgaagc tggcgacaaa gctgctgcac agaaagcatt taacgaaatg
    66661 caaccgatcg tggaccgtca ggctgctaaa ggtctgatcc acaaaaacaa agctgcacgt
    66721 cataaggcta acctgactgc acagatcaac aaactggctt aatcgccaat ttgctgaagc
    66781 tttgtgaaaa agcccgcgca agcgggtttt tttatgcctg ctgcttttgt gatgcgtgag
    66841 agaacacact gccggttgcc agatagtcaa aagagacatg ccgttgtact atctgaactc
    66901 ttcgtggctt aagaatcgta gaaccatcgg ctgatacatt ctgaacaaga atatctactc
    66961 aatctccaga ctcccacaca aagaatatgg atattgtgat acacattgag gtattaaact
    67021 taagaccact ctatttttga attaaattcc tatatacttt aaccattcat aaatatctcc
    67081 tc
//
LOCUS       DAJTYG010000008        65460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTYG010000008 DAJTYG010000000
VERSION     DAJTYG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.008"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(70..549)
                     /gene="folK"
                     /locus_tag="OO568_000574"
     CDS             complement(70..549)
                     /gene="folK"
                     /locus_tag="OO568_000574"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6640058.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(546..1964)
                     /gene="pcnB"
                     /locus_tag="OO568_000575"
     CDS             complement(546..1964)
                     /gene="pcnB"
                     /locus_tag="OO568_000575"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6640059.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2003..2929)
                     /gene="gluQRS"
                     /locus_tag="OO568_000576"
     CDS             complement(2003..2929)
                     /gene="gluQRS"
                     /locus_tag="OO568_000576"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6640060.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLITALGSYLQGR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(2966..3421)
                     /gene="dksA"
                     /locus_tag="OO568_000577"
     CDS             complement(2966..3421)
                     /gene="dksA"
                     /locus_tag="OO568_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6640061.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3599..4303)
                     /gene="sfsA"
                     /locus_tag="OO568_000578"
     CDS             complement(3599..4303)
                     /gene="sfsA"
                     /locus_tag="OO568_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6640062.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4318..4848)
                     /gene="thpR"
                     /locus_tag="OO568_000579"
     CDS             complement(4318..4848)
                     /gene="thpR"
                     /locus_tag="OO568_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6640063.1"
                     /translation="MSEPQRLFFAIDLPAETREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4922..7351
                     /gene="hrpB"
                     /locus_tag="OO568_000580"
     CDS             4922..7351
                     /gene="hrpB"
                     /locus_tag="OO568_000580"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6640064.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAQLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPNDPAN
                     TAPTRRTKKYS"
     gene            7547..10081
                     /gene="mrcB"
                     /locus_tag="OO568_000581"
     CDS             7547..10081
                     /gene="mrcB"
                     /locus_tag="OO568_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6640065.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10301..12544
                     /gene="fhuA"
                     /locus_tag="OO568_000582"
     CDS             10301..12544
                     /gene="fhuA"
                     /locus_tag="OO568_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6640066.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12595..13392
                     /gene="fhuC"
                     /locus_tag="OO568_000583"
     CDS             12595..13392
                     /gene="fhuC"
                     /locus_tag="OO568_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6640067.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13392..14282
                     /gene="fhuD"
                     /locus_tag="OO568_000584"
     CDS             13392..14282
                     /gene="fhuD"
                     /locus_tag="OO568_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6640068.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14279..16261
                     /gene="fhuB"
                     /locus_tag="OO568_000585"
     CDS             14279..16261
                     /gene="fhuB"
                     /locus_tag="OO568_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6640069.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVMLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLIMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16296..17576)
                     /gene="hemL"
                     /locus_tag="OO568_000586"
     CDS             complement(16296..17576)
                     /gene="hemL"
                     /locus_tag="OO568_000586"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6640070.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17801..19222
                     /gene="clcA"
                     /locus_tag="OO568_000587"
     CDS             17801..19222
                     /gene="clcA"
                     /locus_tag="OO568_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6640071.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LIGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19246..19311
                     /locus_tag="OO568_000588"
     CDS             19246..19311
                     /locus_tag="OO568_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640072.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19304..19648
                     /gene="erpA"
                     /locus_tag="OO568_000589"
     CDS             19304..19648
                     /gene="erpA"
                     /locus_tag="OO568_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6640073.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19695..20318)
                     /locus_tag="OO568_000590"
     CDS             complement(19695..20318)
                     /locus_tag="OO568_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6640074.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREISMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20356..21156)
                     /gene="btuF"
                     /locus_tag="OO568_000591"
     CDS             complement(20356..21156)
                     /gene="btuF"
                     /locus_tag="OO568_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6640075.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21149..21847)
                     /gene="mtnN"
                     /locus_tag="OO568_000592"
     CDS             complement(21149..21847)
                     /gene="mtnN"
                     /locus_tag="OO568_000592"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6640076.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPTGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21931..23448
                     /gene="dgt"
                     /locus_tag="OO568_000593"
     CDS             21931..23448
                     /gene="dgt"
                     /locus_tag="OO568_000593"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6640077.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23578..25002
                     /gene="degP"
                     /locus_tag="OO568_000594"
     CDS             23578..25002
                     /gene="degP"
                     /locus_tag="OO568_000594"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6640078.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25157..26313
                     /gene="cdaR"
                     /locus_tag="OO568_000595"
                     /pseudo
     CDS             25157..26313
                     /gene="cdaR"
                     /locus_tag="OO568_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26402..26788)
                     /locus_tag="OO568_000596"
     CDS             complement(26402..26788)
                     /locus_tag="OO568_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6640079.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27102..27926)
                     /gene="dapD"
                     /locus_tag="OO568_000597"
     CDS             complement(27102..27926)
                     /gene="dapD"
                     /locus_tag="OO568_000597"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6640080.1"
                     /translation="MQQLQNIIETAFERRADITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAIIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27957..30629)
                     /gene="glnD"
                     /locus_tag="OO568_000598"
     CDS             complement(27957..30629)
                     /gene="glnD"
                     /locus_tag="OO568_000598"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6640081.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWHIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30691..31485)
                     /gene="map"
                     /locus_tag="OO568_000599"
     CDS             complement(30691..31485)
                     /gene="map"
                     /locus_tag="OO568_000599"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6640082.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31853..32578
                     /gene="rpsB"
                     /locus_tag="OO568_000600"
     CDS             31853..32578
                     /gene="rpsB"
                     /locus_tag="OO568_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6640083.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32836..33687
                     /gene="tsf"
                     /locus_tag="OO568_000601"
     CDS             32836..33687
                     /gene="tsf"
                     /locus_tag="OO568_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6640084.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33834..34559
                     /gene="pyrH"
                     /locus_tag="OO568_000602"
     CDS             33834..34559
                     /gene="pyrH"
                     /locus_tag="OO568_000602"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6640085.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34851..35408
                     /gene="frr"
                     /locus_tag="OO568_000603"
     CDS             34851..35408
                     /gene="frr"
                     /locus_tag="OO568_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6640086.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35500..36696
                     /gene="ispC"
                     /locus_tag="OO568_000604"
     CDS             35500..36696
                     /gene="ispC"
                     /locus_tag="OO568_000604"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6640087.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36885..37643
                     /gene="ispU"
                     /locus_tag="OO568_000605"
                     /gene_synonym="uppS"
     CDS             36885..37643
                     /gene="ispU"
                     /locus_tag="OO568_000605"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6640088.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37656..38513
                     /gene="cdsA"
                     /locus_tag="OO568_000606"
     CDS             37656..38513
                     /gene="cdsA"
                     /locus_tag="OO568_000606"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6640089.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38525..39877
                     /gene="rseP"
                     /locus_tag="OO568_000607"
     CDS             38525..39877
                     /gene="rseP"
                     /locus_tag="OO568_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6640090.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRVAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39907..42339
                     /gene="bamA"
                     /locus_tag="OO568_000608"
     CDS             39907..42339
                     /gene="bamA"
                     /locus_tag="OO568_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6640091.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFREGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42461..42946
                     /gene="skp"
                     /locus_tag="OO568_000609"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42461..42946
                     /gene="skp"
                     /locus_tag="OO568_000609"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6640092.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42950..43975
                     /gene="lpxD"
                     /locus_tag="OO568_000610"
     CDS             42950..43975
                     /gene="lpxD"
                     /locus_tag="OO568_000610"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6640093.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44080..44535
                     /gene="fabZ"
                     /locus_tag="OO568_000611"
     CDS             44080..44535
                     /gene="fabZ"
                     /locus_tag="OO568_000611"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6640094.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44539..45327
                     /gene="lpxA"
                     /locus_tag="OO568_000612"
     CDS             44539..45327
                     /gene="lpxA"
                     /locus_tag="OO568_000612"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6640095.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45327..46475
                     /gene="lpxB"
                     /locus_tag="OO568_000613"
     CDS             45327..46475
                     /gene="lpxB"
                     /locus_tag="OO568_000613"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6640096.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46472..47068
                     /gene="rnhB"
                     /locus_tag="OO568_000614"
     CDS             46472..47068
                     /gene="rnhB"
                     /locus_tag="OO568_000614"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6640097.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47105..50587
                     /gene="dnaE"
                     /locus_tag="OO568_000615"
     CDS             47105..50587
                     /gene="dnaE"
                     /locus_tag="OO568_000615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6640098.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50600..51559
                     /gene="accA"
                     /locus_tag="OO568_000616"
     CDS             50600..51559
                     /gene="accA"
                     /locus_tag="OO568_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6640099.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51658..53799
                     /gene="ldcC"
                     /locus_tag="OO568_000617"
     CDS             51658..53799
                     /gene="ldcC"
                     /locus_tag="OO568_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6640100.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53856..54245
                     /locus_tag="OO568_000618"
     CDS             53856..54245
                     /locus_tag="OO568_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6640101.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54310..55608
                     /gene="tilS"
                     /locus_tag="OO568_000619"
     CDS             54310..55608
                     /gene="tilS"
                     /locus_tag="OO568_000619"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6640102.1"
                     /translation="MTLTLNRPLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55657..55917)
                     /gene="rof"
                     /locus_tag="OO568_000620"
     CDS             complement(55657..55917)
                     /gene="rof"
                     /locus_tag="OO568_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6640103.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55904..56104)
                     /locus_tag="OO568_000621"
     CDS             complement(55904..56104)
                     /locus_tag="OO568_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6640104.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56270..56815
                     /locus_tag="OO568_000622"
     CDS             56270..56815
                     /locus_tag="OO568_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6640105.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56812..57234
                     /gene="arfB"
                     /locus_tag="OO568_000623"
     CDS             56812..57234
                     /gene="arfB"
                     /locus_tag="OO568_000623"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6640106.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     APSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57248..57958
                     /gene="nlpE"
                     /locus_tag="OO568_000624"
                     /gene_synonym="cutF"
     CDS             57248..57958
                     /gene="nlpE"
                     /locus_tag="OO568_000624"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6640107.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58113..58937)
                     /locus_tag="OO568_000625"
     CDS             complement(58113..58937)
                     /locus_tag="OO568_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6640108.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRSIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58990..60708)
                     /gene="proS"
                     /locus_tag="OO568_000626"
     CDS             complement(58990..60708)
                     /gene="proS"
                     /locus_tag="OO568_000626"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6640109.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60819..61526)
                     /gene="tsaA"
                     /locus_tag="OO568_000627"
     CDS             complement(60819..61526)
                     /gene="tsaA"
                     /locus_tag="OO568_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6640110.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61523..61927)
                     /gene="rcsF"
                     /locus_tag="OO568_000628"
     CDS             complement(61523..61927)
                     /gene="rcsF"
                     /locus_tag="OO568_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6640111.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62045..62860)
                     /gene="metQ"
                     /locus_tag="OO568_000629"
     CDS             complement(62045..62860)
                     /gene="metQ"
                     /locus_tag="OO568_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6640112.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62900..63553)
                     /gene="metI"
                     /locus_tag="OO568_000630"
     CDS             complement(62900..63553)
                     /gene="metI"
                     /locus_tag="OO568_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6640113.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63546..64577)
                     /gene="metN"
                     /locus_tag="OO568_000631"
     CDS             complement(63546..64577)
                     /gene="metN"
                     /locus_tag="OO568_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6640114.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64765..65340
                     /gene="gmhB"
                     /locus_tag="OO568_000632"
     CDS             64765..65340
                     /gene="gmhB"
                     /locus_tag="OO568_000632"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6640115.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15460 a        16227 c        18044 g        15729 t
ORIGIN      
        1 aaaattttta ttaaggaaag agaggatgta tgattttcag aaaatattta tattcgtaat
       61 ataaataaat taccatttgc ttaatttgtc aaatgctctt gtatgtaaga cttcacgcaa
      121 cgtctcccca tcaggaaacg ccagctccgg tgcaatctca aacagcggcc acagcataaa
      181 tccacgattc ttcatatcgt agtgcggaac ggtcaggcgt tcggtattta tcacttcatt
      241 accaaacagc atgatatcga gatccagcgt gcgtggtccc cagcgttcag ctttacgaac
      301 acgaccctgt tgcagttcta tgcgctgtgt atggttgagt aactcttccg gggcgggcga
      361 agtttcaagc gccacggcgg cgttgaggta atcgggttga tcttgcggcc ccagcggtgg
      421 ggtgcggtaa aacgaagaaa cggcaagaat gcggctttca gggatatcgc ctaatgcttt
      481 cagggcagca ttgacctgct ccagcggaga ggccagattg ctgcctatgg caatatacgc
      541 cactgtcatg tggtaccctc acgacgcggt gcgcgtttgc gtggacgacg agtacgacga
      601 cgcggtgacg gttcttcatc cagctcgtta agcatccctt tttgatctgg tggtgcggaa
      661 acctggaact caccccacca tttcaccaga cgctgcagtt cagcattacg ttcaacttca
      721 gctcgcaagg ccaacaggtc ataagccgca cggaacttag gatgctccag cagtttccat
      781 gcgcgtttac cctgacgacg ggacatacgc aactgcaact gccagatatc gcgggttaac
      841 gtcgtcagac gtttcgggat tgccagtgaa cggcaggctt cgtccagcac gtcgttcatc
      901 gccagcgcga aagcgtcgtg ataggtcagg ccgctttcct gggcgatctt ctgtgccgtc
      961 tccagcagtg ggtaccagaa catggcggca aacaagaacg ccgggttcac gcgcatatcg
     1021 ttatggatac gcgtatcggt attcttcagc acctgttcaa tgatccgctc catcgggctg
     1081 tcgccatttt ccgtgaagta gcgggtaatg gtcgggaaca gcggctggaa cagatgatat
     1141 tcacacaaca gcttataggt ttcgtaaccg tagcccgctt gtagcagttt aagcgattct
     1201 tcaaacaggc gtgccggtgg gatatcgttc agcagggtag cgaggcgagg gatcggttct
     1261 gcggtttccg ggctgatgcg catacccaat ttggcggcaa aacgtaccgc gcgcagcata
     1321 cgtaccggat cttcacggta gcgcgtttcc gggttaccaa tcagacggat aacgccgtcc
     1381 ttcagatcct tcataccgcc aacgtaatca cggacggtaa aatccgctac gctgtaatac
     1441 aggctgttga tagtgaaatc gcggcgctgg gcgtcttctt cgatggagcc gaaaatgttg
     1501 tcgcgcagca acatgccgtt ttgcccgcgt tgggaggtcg tgcggtcgct gacgttacct
     1561 tcgtggtgtc cacggaaggt cgcaacttcg ataatctccg ggccaaacat tacatgagcc
     1621 agacggaaac ggcgacccac caggcggcag ttacggaaca gtttgcgcac ctgctcaggc
     1681 gtggcgttag tggttacgtc aaaatctttc ggctttttgc caagtaacag gtcgcgcacg
     1741 ccgccgccaa ccagccaggc ttcgtatccc gctttgttga gcctgtacat taccttcagg
     1801 gcattttcac tgatatcttt gcgggaaata gcatgctgct cacgcgggat cactgtcacc
     1861 tgtggacggg cgactgcctg ttcagcctcg ctttcctcgc ggcttagcac cttgcggcaa
     1921 aaattagcga ctcgggtaaa aatagtacac ctcggtagtg tcaaacatca ttcaggacaa
     1981 aaaaatagcg gctaatcata gctcagcatg acgcatttga gaatgttgaa tttacaattg
     2041 ccgactcggg cacggcggtc agcatccagt ttttgacggc tgactgaagg atttgctcga
     2101 cgctgaaatc ctgccagtgt gcttctgcct gctgccccag aaattgaagt gccgcgatta
     2161 gtaccgggcg tggatcgcct ttcggcaacg caggcgcatg attctgcttg gaaagtttag
     2221 cgccttgtgg attaagcgcc agcggcagat gaatgtaatc tggcactttc cagccaaaaa
     2281 gctggtacag cgagatttgc cttactgttg gttcaatcag atcagcccca cgcactattt
     2341 ctgtaacgcc ctggaaatga tcatcaacca caacagccag gttgtaggcg aacaacccat
     2401 cacggcgatg aatgataaaa tcttcccgtg ccagtttttc gtcggcgtga ataatgcctc
     2461 gcagcaggtc agtaaattgc gtgaccggat gctgctggcg gatacgcact gcggcgttgt
     2521 ctggtccatg atgcaacacc cggcaatgac cgtcgtaaat accgccaatg ctttgaatac
     2581 gcgcacgcgt acaggtgcag taataactta gtccttgttc atgtaaccag gcgagtgctt
     2641 cacgataggc gtcgtgacgt tgcgattgcc agagaacatc gccgtcccag tgcagaccgt
     2701 aatgttccag ctggcgcagg atagtttctg cggcaccggg aacttcacga ggcgggtcga
     2761 tatcttctat gcgtaccagc cagcgacctt gccgggcgcg accctgcaaa tagctgccga
     2821 gcgcggtgat cagagagcca aaatgaagct cgccggaagg agagggggcg aagcggccaa
     2881 tatactgtgt gtctgtcatc tctttgaaca aaaaataagg cgggagcatt tcccgcctgt
     2941 ggtaaacgtg atggaacggc tgtaattagc cagccatctg tttttcgcga atttcagcca
     3001 gcgttttgca gtcgatgcac agatcggctg tcgggcgcgc ttccagacgg cgaataccaa
     3061 tttcaacacc gcaggattcg cagtagccga aatcttcgtc ttccactttt ttcagcgtct
     3121 tctcgatctt tttgatcagc ttacgctcgc gatcgcggtt acgcagttcg aggctgaact
     3181 cttcttcctg ggctgcacgg tctaccgggt ccgggaagtt ggctgcttca tcctgcatat
     3241 gtgtaacggt gcgatcgact tcatccctga gttgattacg ccatgcttcc agaatacgac
     3301 ggaagtgcgc cagctgggct tcattcatat actcttcgcc cggcttctcc tgatatggtt
     3361 ccaccccagc gatggcgaga atactcaggg acgatgtttt acggttttgc ccttcttgca
     3421 tgttgcttct ccttaacacg cactatcgat ccccatgttc gggggaaaaa tgaggccgct
     3481 ataaatagca gatgcttttc cggatagcaa ttatctaaac gtaacacttg acaactgtgt
     3541 gaggaaaagc gtattcgcgc acgcgaccag aatgtaaatt aaccagttac ttactttact
     3601 acaatgtaac cggcagtgat tttttaagag ccatgccttc agcagaaatt tccgctttgt
     3661 aagccagaat ttctaccccc ctctgttgag cttctgacaa tagttgcgcg tatttctcat
     3721 cgatgtggcg cgcgggtgaa aaccgtgtaa tggctgaatg cagcacggcg aaaaagataa
     3781 ccgcacgctg gccttcagcc gctacgctca tcaactcccg aaggtgtttc tgacctcgtt
     3841 cagtgaccgc atcgggaaaa tatccctgtt cgttctccgc taacgtaacc gatttcactt
     3901 caatatagca gtctggacgc gaatccgcct gcaacataaa gtcaatacgg ctgcgttcgg
     3961 agccgtattt tacttcgctt ttcagcgagc tatagcctga cagttctgaa attgattcat
     4021 taaggatagc ctctttcgtc aacctgttag cccaaagcgt attgacgcaa ataaatgcgc
     4081 cgctctggct ttgagttaat tcccaggtgt gtgggtattt ccgtttggtg ttgtctgaag
     4141 tcgaatacca gacggtatcg ccaggcgttg cacaaccggt catcgcaccc gtattcgggc
     4201 agtgtagcgt taattcgcga ccatcgggtg tgatcacatc ggctaaaaaa cgtttgtaac
     4261 gctgaattag cgtcgcgcgc tgtagagggg gagaaaattc cattgcgaca atccttgtta
     4321 ttgcgttagc gcccagcgtt ttagcggcgt gtagcgtgtg cgtccacggg caaacgagga
     4381 ggcgtaaagg gtgaactccg tcaccgcata agaccagtta aaacccggcg gcgggaatgt
     4441 caccgcctcg ctggcgtcgc gcaataaggt aatatgtgga tgaaacggac gattgctttg
     4501 aaaacaaccg ctgcgggcag cctgtgaacg gagcatattc gccagctgga ttaagccgcg
     4561 tggaggctga cgcatcccta accacaccac acgcgaacgc agccattgtc cggcgtcatc
     4621 aagcgtgagt gtgaaacctg gttgacgaat ccgtccggct aaaagagaaa gcgccttctc
     4681 tttctctgcg ctcacttcgc ctaaaaatgc cagagtcaga tgcaaattat cggcggcgac
     4741 cggacgtccc gcctcaggtg ggaagtgtgt ggcgcgccaa tggataatct gttcgcgggt
     4801 ttctgcaggt aagtcgatag caaagaacag acgttgcggt tcagacatgt gaggcactcg
     4861 gttatgaatt accgcgatgc tacaatgtgg cgcgaagaat gttaaccctc tggagcgttt
     4921 tgtgtcgtcg ttgcccgttg ctgccgtctt acctgaatta cttaccgctc tcgattgtgc
     4981 gccgcaggta ttattaagtg cgccgaccgg ggccgggaaa tcaacctggc tgccgctgca
     5041 actgctggcg catcccggca ttaacgggaa aattatcctg ctggagccgc gtcgtctggc
     5101 ggcgcgtaac gtcgcgcaac ggctggcgga gctgcttaac gaaaagccag gcgataccgt
     5161 tggctaccgg atgcgtgcgc aaaactgcgt cgggccgaat acccgcctgg aagtggttac
     5221 cgaaggcgtg ctgacgcgga tgatccagcg tgacccggaa ctgagcggtg ttggattggt
     5281 gatccttgat gagtttcatg agcgcagttt gcaggcggat ttggcgttgg cgctgttact
     5341 cgatgtgcaa caaggtctgc gtgatgacct taaactgctg attatgtcgg ctacgctgga
     5401 caatgaccgc ttgcagcaaa tgctgccgga agcgcccgtc gtcatctcag aagggcgctc
     5461 gtttccggtt gaacgccgtt atttaccgct gcccgcgcat cagcgttttg acgaagccgt
     5521 tgcggtagcc accgccgaaa tggtgcgtca ggaaagtgga tcattactgt tatttttacc
     5581 tggcgtcgga gaaattcagc gcgtgcagga acaactggct tcgcgcatcg gcagtgatgt
     5641 ggtgctctgc ccgctgtatg gcgcgttgtc actgaacgat cagcgaaaag cgatcctccc
     5701 ggcaccgcaa gggatgcgca aagtggtgct ggcgaccaat attgctgaaa ccagtttaac
     5761 catcgaaggt attcgtttgg tggtggattg tgcccaggag cgtgtggcgc gctttgatcc
     5821 gcgcacgggg cttacgcggt tgattactca acgcgttagt caggcatcca tgacgcagcg
     5881 tgccgggcgc gccgggcgtc tggagccggg tatcagcctg catttaatcg ccaaagaaca
     5941 agcagaacgc gccgcggcgc aaagtgagcc ggagatctta caaagcgatc tttccggttt
     6001 gctgatggaa ttactgcaat ggggatgcag cgatccggcg cagatgagct ggctggatca
     6061 accgccaacg gtgaatctac tggccgcgaa acgcctgtta caaatgctgg gggcgctgga
     6121 gggtgaacgg cttagtgcgc aagggcaaaa aatggcagcg ctgggtaacg atccgcgttt
     6181 agcggcaatg ctggttaacg caaagagcga tgacgaagct gctaccgcgg caaaaattgc
     6241 cgccattctc gaagagccgc cacggatggg caatagtgac ctgggcgtgg cgttttcgcg
     6301 caatcaacca gcctggcagc aacgtagtca gcaactgtta aaacgcttaa acgtacgcgg
     6361 cggtgaggca gacagttcgc ttatcgcgcc gctacttgcc ggggcgtttg ccgatcgcat
     6421 tgctcatcgc cgtgggcaag atggacgcta tcaactggca aacggcatgg gagcgatgct
     6481 cgatgccgac gacgcgctaa gccgccacga atggttgatc gcacagttat tattgcaggg
     6541 cagcgcctcg ccggatgcgc ggattttact ggcgctaccg gtcgatattg atgaattagt
     6601 acaacgctgt ccgcagctgg tgcagcaatc tgacaccgtg gaatgggatg acgcgcaagg
     6661 tacgctgaaa gcctggcgtc ggctacaaat cggtcagttg acggtgaaag tgcagccgct
     6721 ggcgaaaccg tcagaagacg agttgcatca ggcgatgctt aatggcatcc gtgataaagg
     6781 tttaagcgtg ctcaactgga cggcggaagc ggaacagcta cgcttgcgtt tgttatgcgc
     6841 cgcaaagtgg ttgccggaat atgactggcc agcggttgat gatgaaagtt tgttggcgac
     6901 gctggaaacg tggctgctgc cacatatgac cggcgtacat tcactacgcg gcctgaaatc
     6961 actcgacatt tatcaggcac tacgcggatt acttgattgg ggaatgcagc aacgtctgga
     7021 tagtgaattg cctgcgcatt acactgtgcc gacgggaagc cggatcgcca ttcgttatca
     7081 tgaagataac ccgcccgcgc tggcggtgag aatgcaggag atgtttggtg aggccactaa
     7141 tccgacgatc gcccaggggc gcgtgccgct ggtgctgaag ttgctttccc ctgcccaaag
     7201 gccgctgcaa atcacgcgtg atttgggggc cttctggaaa ggggcgtatc gtgaggtgca
     7261 aaaggagatg aaagggcgtt atcccaaaca cgtctggccg aacgacccgg caaacacggc
     7321 accgacgcga cggacgaaaa agtattcgta aggttggttt tctccctctc cctgtgggag
     7381 agggccgggg tgagggcatc agcgcgcacg ttcaccctca ccctaaccct ctccctcaag
     7441 ggagagggga ccgatcgagc acaaattttg agagatatct tcttctgtct tgtaacagaa
     7501 gaacagaaaa tcgggctttt gcgcctgaat attgcggaga aaaagcatgg ccgggaatga
     7561 ccgcgagcca attggacgca aagggaaacc gacgcgtccg gtcaaacaaa aggtaagccg
     7621 tcgtcgttac gaagatgacg atgattacga cgattatgat gactatgagg atgaagaacc
     7681 gatgccgcgc aaaggtaagg gcaaaggcaa agggcgtaag cctcgtggca aacgcggctg
     7741 gctatggcta ctgctaaaac tggctatcgt ttttgccgtg ctgatcgcca tttacggcgt
     7801 ttatctcgat caaaaaattc gtagccgtat tgatggcaag gtctggcaac tgcccgcggc
     7861 agtttatggc cgaatggtca accttgaacc agatatgacc atcagcaaga acgaaatggt
     7921 gaagctgctg gaggcaacac agtatcgtca ggtgtcgaaa atgacccgtc ctggcgaatt
     7981 taccgtgcag gccaacagca ttgagatgat ccgccgtccg tttgatttcc cggacagtaa
     8041 agaagggcag gtgcgtgcgc gtctgacctt tgatggtgat catctggcga ccatcgtcaa
     8101 tatggagaac aaccgtcagt tcggtttctt ccgtcttgat ccgcgtctga tcaccatgat
     8161 ctcctcgcca aacggtgagc agcgtttgtt tgtgccgcgc agtggtttcc ctgatttgct
     8221 ggtggatact ttgctggcga cagaagaccg tcatttttac gagcatgatg gaatcagtct
     8281 ctactccatt gggcgtgcgg tgctggcaaa cctgaccgca ggtcgcacgg tgcagggtgc
     8341 gagtacgctg acgcaacagc tggtgaaaaa cctgttcctc tccagcgagc gttcttactg
     8401 gcgtaaagcg aacgaagctt acatggcgct gatcatggac gcgcgttaca gcaaagaccg
     8461 tattcttgag ctgtatatga acgaggtgta tctcggtcag agcggcgaca acgaaatccg
     8521 cggcttcccg ctggcaagct tgtattactt tggtcgcccg gtagaagagc taagcctcga
     8581 ccagcaggcg ctgttagtcg gtatggtgaa aggggcgtcc atctacaacc cgtggcgtaa
     8641 cccgaaactg gcgctggagc gacgtaatct ggtgctgcgt ctgctgcaac agcaacagat
     8701 tattgatcaa gaactctatg acatgttgag tgcccgtccg ctgggggttc agccgcgtgg
     8761 tggggtgatc tctcctcagc cagcctttat gcagttggtg cgtcaggagt tgcaggcaaa
     8821 actgggcgat aaggtaaaag atctctccgg cgtgaagatc ttcactacct ttgactcggt
     8881 ggcccaggac gcggcagaaa aagccgccgt ggaaggcatt ccggcactga agaaacagcg
     8941 taagttgagc gatcttgaaa ctgcgattgt ggtcgtcgac cgctttagtg gtgaagttcg
     9001 tgcgatggtc ggaggttctg agccgcagtt tgcgggctac aaccgtgcga tgcaggcgcg
     9061 tcgttcgatt ggttcccttg caaaaccagc gacttatctg acggctttaa gccagccgaa
     9121 aatctatcgc ctgaacacgt ggattgccga tgcgccaatc gcgctgcgtc agccgaatgg
     9181 ccaggtctgg tcaccgcaga acgatgaccg tcgttatagc gaaagcggca gagtgatgct
     9241 ggtggatgcg ttgacacgtt cgatgaacgt gccgacggta aatctgggga tggcgctggg
     9301 gctgcctgcg gttacggaga cctggatcaa actgggcgta ccgaaagatc agttgcatcc
     9361 ggttccggca atgctgctgg gggcgttgaa cttaacgcca atcgaagtgg cgcaggcatt
     9421 ccagaccatc gccagcggtg gtaaccgtgc accgctttct gcactgcgtt cggtaatcgc
     9481 ggaagatggt aaggtgctgt atcagagctt cccgcaggca gaacgcgctg ttccggcgca
     9541 ggcggcgtat ctgacactat ggaccatgca gcaggtggta caacgtggta cgggtcgtca
     9601 gcttggggcg aaatacccga acctgcatct ggcagggaaa acagggacta ccaacaataa
     9661 cgtagatacc tggtttgcag gcattgacgg cagcacggtg accatcacct gggtaggccg
     9721 tgataacaac cagccgacca aactgtatgg agccagcggg gcaatgtcga tttatcagcg
     9781 ttatctggct aaccagacgc caacgccgct gaatcttgtt ccgccagaag atattgcaga
     9841 tatgggcgtg gactacgacg gcaactttgt ttgcagcggt ggcatgcgtg tcttgccggt
     9901 ctggaccagc gatccgcaat cgctgtgcca gcagagcgag atgcagcagc agccgtcagg
     9961 caatccgttt gatcagtctt ctcagccgca gcaacagccg caacagcaac ctgctcagca
    10021 agagcagaaa gacagcgacg gtgtagccgg ttggatcaag gatatgtttg gtagtaatta
    10081 acatctaagc gtgaaatacc ggatggcgag ttgccatccg gtaaaataac atcccatcta
    10141 agatattaac cctttctttt catctggttg tttattaacc cttcaggaac gctcagattg
    10201 cgtaccgctt gcgaacccgc cagcgtttcg aatattatct tatctttata ataatcattc
    10261 tcgtttacgt tatcattcac tttacatcag agatatacca atggcgcgtt ccaaaactgc
    10321 tcagccaaaa cactcactgc gtaaaatcgc agttgtagta gccacagcgg ttagcggcat
    10381 gtctgtttat gcacaggcag cggttgaacc gaaagaagac actatcaccg ttaccgctgc
    10441 acctgcgccg caagaaagcg catgggggcc tgctgcaact attgcggcgc ggcagtcagc
    10501 taccggcact aaaaccgata cgccgattca aaaagtgcca cagtctattt ctgttgtgac
    10561 cgccgaagag atggcgctgc atcagccgaa gtcggtaaaa gaagcgctta gctacacgcc
    10621 gggtgtctct gttggtacgc gtggcgcatc caacacctat gaccatctga tcattcgcgg
    10681 ttttgcggca gaaggccaaa gccaaaataa ctatctgaat ggcctgaagt tgcagggcaa
    10741 cttctataac gatgcggtca ttgacccgta tatgctggaa cgcgctgaaa ttatgcgtgg
    10801 cccggtttcc gtgctttacg gtaaaagcag tcctggcggt ctgttgaata tggtcagcaa
    10861 gcgtccgacc accgaaccgc tgaaagaagt tcagtttaaa gccggtactg acagcctgtt
    10921 ccagactggt tttgacttta gcgatgcgtt ggatgatgac ggtgtttact cttatcgcct
    10981 gaccggtctt gcgcgttctg ccaatgccca gcagaaaggg tcagaagagc agcgttatgc
    11041 tattgcaccg gcgttcacct ggcgtccgga tgataaaacc aatttcacct tcctttctta
    11101 cttccagaac gagccggaaa ccggttatta cggctggttg ccgaaagagg gaaccgttga
    11161 gccgctgccg aacggtaagc gtctgccgac agactttaat gaaggggcga agaacaacac
    11221 ctattctcgt aatgagaaga tggtcggcta cagcttcgat cacgaattta acgacacctt
    11281 tactgtgcgt cagaacctgc gctttgctga aaacaaaacc tcgcaaaaca gcgtttatgg
    11341 ttacggcgtc tgctccgatc cggcgaatgc ttacagcaaa cagtgtgcgg cattagcgcc
    11401 agcggataaa ggccattatc tggcacgtaa atacgtcgtt gatgatgaga agctgcaaaa
    11461 cttctccgtt gatacccagt tgcagagcaa gtttgccact ggcgatatcg accacaccct
    11521 gctgaccggt gtcgacttta tgcgtatgcg taatgacatc aacgcctggt ttggttacga
    11581 cgactctgtg ccactgctca atctgtacaa tccggtgaat cccgatttcg acttcaatgc
    11641 caaagatccg gcaaactccg gcccttaccg cattctgaat aaacagaaac aaacgggcgt
    11701 ttatgttcag gatcaggcgc agtgggataa agtgctggtc accctaggcg gtcgttatga
    11761 ctgggcagat caagaatctc ttaaccgcgt tgctgggacg accgataaac gtgatgacaa
    11821 acagtttacc tggcgtggtg gtgttaacta cctgtttgat aatggtgtaa caccttactt
    11881 cagctatagc gaatcgtttg aaccttcttc gcaagttggg aaggatggta atattttcgc
    11941 accgtctaaa ggtaagcagt atgaagtcgg cgtgaaatat gtaccggaag atcgtccgat
    12001 tgtagttact ggtgccgtgt ataatctcac taaaaccaac aacctgatgg cggaccctga
    12061 gggttccttc ttctcggttg aaggtggcga gatccgcgca cgtggcgtag aaatcgaagc
    12121 gaaagcggcg ctgtcggcga gtgttaacgt agtcggttct tatacttaca ccgatgcgga
    12181 atacaccacc gatactacct ataaaggcaa tacgcctgca caggtgccaa aacacatggc
    12241 ttcgctgtgg gctgactata ccttctttga cggtccgctt tcaggtttga cgctgggcac
    12301 cggtggtcgt tatactggct ccagctatgg tgatccggct aactccttta aagtgggaag
    12361 ttatacggtc gtggatgcgt tagtgcgtta tgatctggcg cgagtcggca tggcgggctc
    12421 caacgtggcg ctgcatgtta acaacctgtt cgatcgtgaa tacgtcgcca gctgctttaa
    12481 cacttatggc tgcttctggg gcgcagaacg tcaggtcgtt gcaaccgcaa cctttcgttt
    12541 ctaatttctc ttttggggca cggatttccg tgcccatttc acaagttggc tgttatgcag
    12601 gaatacacga atcattccga taccactttt gcactgcgta atatctcctt tcgtgtgccc
    12661 gggcgcacgc ttttgcatcc gctgtcgtta acctttcctg ccgggaaagt gaccggtctg
    12721 attggtcaca acggttctgg taaatccact ctgctcaaaa tgcttggtcg tcatcagcct
    12781 ccgtcagaag gggagattct tcttgatgcc cagccgctgg aaagctggag cagcaaagcg
    12841 tttgcccgca aagtggctta tttgccgcag cagcttcctc cggcagaagg gatgaccgtg
    12901 cgtgaactgg tggcgattgg tcgttacccg tggcatggcg cgctggggcg ctttggtgcg
    12961 gcagatcgcg aaaaggtcga ggaagctatc tcgctggttg gcttaaaacc gctggcgcat
    13021 cggctggtcg atagtctctc tggcggcgaa cgtcagcggg cgtggatcgc catgctggtg
    13081 gcgcaggata gccgttgtct gttgctcgac gaaccgactt cggcgctgga tatcgcccac
    13141 caggttgatg tgctggcgct ggtgcaccgt ttaagtcagg aacgtggcct gacggtcatt
    13201 gccgtgttgc acgatatcaa tatggcggca cgctactgtg attatctggt cgccctgcgc
    13261 agtggtgaaa tgattgctca gggaacgcct gcggaaatta tgcgcggcga aaccctcgaa
    13321 atgatttatg gcatcccgat gggtattttg ccgcatccgg cgggtgctgc acctgtgagt
    13381 tttgtttatt gatgagcggc ttacctctta tttcgcgccg tcgactgtta acggcgatgg
    13441 cgctttctcc gttgttatgg cagatgaata ccgcccacgc ggcggctatt gaccccaatc
    13501 gtattgtggc gctggagtgg ttgccggtgg aattactgct ggcgctcggc atcgtgcctt
    13561 acggcgtggc ggataccatc aactatcgcc tgtgggtcag cgaaccacca ttgccggact
    13621 cagtgattga cgtcggtttg cgtacagaac ctaaccttga actgctgacc gaaatgaaac
    13681 cgtcgtttat ggtctggtcg gcaggatatg gcccttcatc agaaatgctg gcacgtattg
    13741 cgccgggtcg cggatttaac ttcagtgacg gcaaacatcc gttggcgatg gcgcgtaaat
    13801 cgctgacgga aatggcagat ttacttaacc tgcaaagcgc agcggaaacg catttagcgc
    13861 aatacgaaga ctttatccgc agcatgaaac cccgctttgt gaagcgtggt gcacgcccgt
    13921 tactgctgac gacgcttatc gatccgcgcc atatgctggt cttcggtcca aacagcttgt
    13981 tccaggaaat tctcgatgag tacggcatcc caaatgcctg gcaaggggaa accaacttct
    14041 ggggcagtac cgccgtcagt atcgatcgtc tggcagcgta taaagacgtt gatgtgctct
    14101 gttttgatca cgacaacagc aaagacatgg atgcgctaat ggcaaccccg ctgtggcagg
    14161 ccatgccgtt tgtccgtaca gggcgctttc agcgcgtacc cgcagtctgg ttttatggtg
    14221 caacgctttc ggcaatgcac tttgtgcgtg ttctggataa cgccatcgga ggtaaagcgt
    14281 gagtaaacga attgcgcttt tcccggcgtt attgctggcg ctgttagtga ttgtcgctac
    14341 ggcgctcacc tggatgaact tctcacaggc gctgccgcgc agtcagtggg cgcaggccgc
    14401 ctggtcgccg gatattgacg tcatcgagca gatgattttt cactacagct tgttgccgcg
    14461 tctggcgatt tcgctgctgg tgggcgcggg cctggggctg gtgggcgtgc tgtttcagca
    14521 agtactgcgt aacccgctgg cggaaccgac gacgttgggc gttgcaacag gcgcgcaact
    14581 ggggattacc gtcaccacgc tctgggcgat ccctagcgcg atggcgagcc agtttgctgc
    14641 gctggcaggg gcttgcgttg ttggcttaat tgtctttggc gtcgcgtggg ggaaacggct
    14701 gtcgccggta acgctgattc tcgcggggtt ggtagtgagc ctttattgcg gcgcaatcaa
    14761 tcagttactg gttatcttcc atcatgacca gttgcaaagc atgtttctgt ggagcactgg
    14821 aacgctgacg caaaccgact ggggcggtgt tgagcgttta tggccgcagt tgctgggcgg
    14881 tgtgatgctg acgttgctgc tacttcgtcc gttaaccctg atggggcttg atgatggcgt
    14941 ggcgcgcaat ctcgggctgg ccttgtcgct tgcgcgtctg gcggcgctgt cgctggcgat
    15001 tgtcatcagt gcgctgctgg tgaacgctgt ggggattatc ggctttatcg ggttgttcgc
    15061 gccgctgctg gcaaaaatgc tgggggcgcg gcgtctgctg ccacgactga tgctggcgtc
    15121 gttgattggt gcgctgatcc tctggctttc cgatcaaatc atcctctggc tgactcgcgt
    15181 gtggatggaa gtgtccaccg gttcggtcac tgcgttgatc ggtgcgccgc tgctactgtg
    15241 gctgctgccg cgtttacgca gcattagcgc gccggatatg aaggttaacg atcgtgtcgc
    15301 gactgaacgc caacatgtgc tggcgtttgc cctcgcgggc ggcgtgctgt tgttgatggc
    15361 tgtggtggtg gcgctgtcgt ttggtcgtga tgcgcacggc tggacgtggg cgagcggggc
    15421 gttgctcgag gatttaatgc cctggcgctg gccgcgaatt atggcggcgc tgtttgcggg
    15481 cgtcatgctg gcggtagcgg gctgtattat tcagcgactg accggcaacc cgatggcaag
    15541 cccggaagtg ctggggatta gctccggcgc ggcgtttggt gtggtgttaa tgctgtttct
    15601 ggtaccgggt aatgcctttg gctggttgtt acccgcaggc agtctcgggg cggcggtgat
    15661 gctgttgatc attatgatcg ccgccggacg cggtggattt tccccacacc gcatgttact
    15721 ggcggggatg gcgttaagta ccgcgttcac catgcttttg ataatgttgc aggcaagtgg
    15781 tgacccgcga atggcgcaag tgctgacctg gatttccggt tcgacctaca acgcgaccga
    15841 tgctcaggtc tggcgcaccg gaattgtgat ggtgattttg ctggcgatta ctccgctgtg
    15901 ccgccgctgg ctgaccattt taccgctggg tggtgatacc gcgcgggcgg tagggatggc
    15961 gctgacgccg acgcgaattg cgctgctgct gttagcggct tgcctgacgg cgaccgcgac
    16021 gatgactatt ggaccgttga gttttgttgg tttaatggca ccgcatattg cgcggatgat
    16081 gggctttcga cggacgatgc cacatatcgt aatttcggcg ctggtgggtg gtttactgct
    16141 ggtgttcgct gactggtgtg ggcggatggt gctgtttcca ttccagatcc cggcggggct
    16201 gctgtcgacc tttatcggtg cgccatattt tatctatttg ctgagaaagc agagccgtta
    16261 atagaatgcc tcgcccggtg ggcgaggcaa aattatcaca acttcgcgaa cacccgacgt
    16321 gcagcatcga tggtgttatt gatatcttcc atgctgtgcg ccacagacat aaagcccgct
    16381 tcaaacgctg acggtgccag gtaaacacct tcatccagca tcatatggaa gaaacgctta
    16441 aaacgctcga cgtcacaggc catcacatcc tgatagcacg tcacggactc ggcgtcggta
    16501 aagaagatac cgaacatacc accgacgtgg ttaacgacca gcggaattcc cgcttcttct
    16561 gccgcttcca gcagaccttc tgccagacgt gttgtcagct catccagcgt ttcgtgaatg
    16621 cccggctgcg cgacttcatt cagacaggcg aaacccgctg ccatcgcaat cgggttaccg
    16681 gaaagcgtac ccgcctgata gaccggacct gtcggggcca gcgcatccat tacatcacga
    16741 cgaccaccga atgcgcctac cggcattcca ccgccgatga tttttccgag gcaggttaaa
    16801 tctggcacta cgccgaaata atcctgtgcg ccagctagcg ctacgcggaa accggtcatc
    16861 acttcatcga tgatcagcaa cgcgccaaat tcgtcgcaca gcgcacgcag acctggcagg
    16921 aactctggca gcggcggaac acagttcata ttgcctgcca ccggctcgac gataatacag
    16981 gcaatctctt gcgggtattg ctcaaacgcg gcgcgtacag aggccagatc gttataagta
    17041 caggttaagg tatgtttggc gaaatctgcc ggaacgcccg gcgagtttgg ctggcctaac
    17101 gtgagtgcgc cagaaccggc tttcaccagc aggcagtcag cgtgaccgtg gtaacaacct
    17161 tcaaatttaa tgattttgtc gcgaccggta aaaccacggg ccaggcggat ggcgctcatc
    17221 gtcgcctcgg tgccggagtt caccatgcgc accatatcca tggtcgggac cagttcggtc
    17281 accagttgcg ccattttcac ttccatttcg gtcggtgcac caaagcttaa accacgctcg
    17341 gcggcttcaa tcacggcatt gcggatcgcc ggatggttat ggcccagcac catcggcccc
    17401 caggaaccga cataatcgat ataggctttg ccatcaacat cgtacagata agcgccgtcc
    17461 gctttttcga taaacagtgg agtgccgccc acgccagtaa aggcgcgaac aggggagttc
    17521 acaccgccag ggatcagctc gcgcgctgcg ctgtaaagat tttcagactt actcatggag
    17581 gggtcctgat tcgtagaaaa agtgaatggc tgctattcta tgttattcat aacaagttaa
    17641 atactcgtca aacatcaggc tgcttgtact ggtgcaatcc tgatttctta gagtataaaa
    17701 gttttgtgca tttgaaacat tacgctttgc aaaggatttt catggaacgt gcgagtaaaa
    17761 tgccgtcatc ttatttgtat gaccaataag tgatcattgg atgaaaactg atactccctc
    17821 tttagaaaca ccgcaggccg cgcgcctgcg acgcagacaa ctgattcgcc aacttcttga
    17881 gcgcgataaa accccgttag ccattttgtt tatggcggca gtcgtcggca cgcttatcgg
    17941 gctggctgcg gttgcttttg acaaaggtgt cgcctggttg cagaaccaac gtatgggggc
    18001 gctggtacat actgctgata attatccgct actgttaacc gtcgcttttc tctgttcggc
    18061 ggtgctggcg atgttcggct actttttggt gcgcaaatac gcgcccgaag cagggggatc
    18121 ggggatcccg gaaattgaag gggcgctgga agatcaacgt cctgttcgct ggtggcgtgt
    18181 attgccggtg aaattctttg gcgggctggg gacactcggc ggaggcatgg tgctagggcg
    18241 cgaaggacca accgtgcaga tcggcggcaa tatcggtcgc atggtgcttg atattttccg
    18301 cctgaaaggt gatgaagctc gccatacgct gctggcaacc ggtgctgctg cggggctggc
    18361 tgcggccttt aacgcgccgc tggcgggtat tttgtttatt atcgaagaga tgcgtccgca
    18421 gtttcgctat acgttaattt cgattaaagc ggtatttatt ggcgtcatta tgtcgaccat
    18481 tatgtaccgg atttttaatc atgaagttgc gttgattgac gtcggtaaac tttctgatgc
    18541 gccgcttaat acgctgtggc tttatctgat cctcggtatt atttttggca ttttcggccc
    18601 tatttttaat aaatgggtgc tggggatgca ggatttgctg caccgtgtgc acggcggcaa
    18661 tattaccaaa tgggtactga tggggggggc gattggcggt ctgtgtggat tgctggggtt
    18721 tgtggcacca gcaacgtcgg gcggcggttt taacctgatt cctatcgcta ccgcggggaa
    18781 tttcagcatg ggaatgctgg tgtttatctt cgtcgctcgg gtcattacca ccttactctg
    18841 cttctcttcc ggcgcgccgg gcggtatttt tgccccgatg ctggcgctgg gtactgtgct
    18901 gggaaccgct ttcggaatgg ttgccgttga gctgtttccg caatatcacc tggaggcggg
    18961 gacgtttgct attgccggaa tgggggcatt actggcggca tctattcgcg cgccgttaac
    19021 ggggatcatt ctggttctgg agatgaccga taactaccag ctcattttgc caatgattat
    19081 taccggtctt ggcgcaacac tattagcgca atttaccggc gggaaacctc tatactcggc
    19141 gattcttgcg cgcacgctgg caaaacagga agctgagcaa ctggcgcgaa gcaaggccgc
    19201 atcagccagc gagaatactt gaacgaaata ccagggtatt agataatggc gattattatt
    19261 gggttagaat ttgcccgatt gccgatgtcg tttggagcaa aatatgagtg atgacgtagc
    19321 actgccgctg gagtttaccg acgcagcagc caacaaagtt aaaagcctga tcgctgacga
    19381 agataacccg aatctgaaat tacgcgtgta tatcaccggt ggcggttgca gcggcttcca
    19441 gtatggtttc acctttgatg atcaggtgaa cgaaggcgat atgaccatcg aaaaacaggg
    19501 cgttggcctg gtggttgatc cgatgagcct gcaatatctg gtcggcggtt ccgttgacta
    19561 caccgaagga ctggaaggtt ctcgtttcat cgtgaccaac ccgaacgcga aaagcacctg
    19621 cggttgcggt tcttccttta gcatctaatc tgttgttcat gtgccggata aactatccgg
    19681 cacatcttct ctcttcaacg cccattctca tccagcgcaa acgtcggtag cttaagatgc
    19741 caacgaatag ccgccagccg aatcaatagc gtcacgacca tgcccatcat actggctgtt
    19801 tccagtggta cggaaaatgt gtaataagcc gtagcgtgga caataccgcc gataatacag
    19861 gcagttgcgt agatttctgt acgtaaaatc atggagattt cgcgggccag tacatcacga
    19921 atgatcccgc cgccaacgcc agtaatgacg cccatacaaa ccgcgattaa cggaccggct
    19981 tccgcattaa aggctttatt cacgccaatg ccgacaaaca ccgccagacc aacggcgtcc
    20041 agcaccggca acatccattt tggtaagcgt cttggctggc gcaccagcac gatggtcagc
    20101 atgctggtga ccatggcaac gaccagatcg gtgggatctt tcacccaaaa taccgggccg
    20161 tgatccagcg ccatgtcgcg aattgttccg tcgcctactg cggtaaccac gcccagtacc
    20221 agaacaccaa aagggtccat acgcaatttt ccggctaaca aaacgccgga gatggcaaat
    20281 accgctgtgc cgactatatc cagccaatag acgagcattg ttcaatcccc actgagcacc
    20341 tgaaaaggtc aggcactaat ctacctgtga aagcgcatta cagagctgtt gtgctgcgag
    20401 gataatacgt gggcttgcac gttcaaacca gtcactcgtg agaggaataa cgggaatttt
    20461 gagctgttca ccccagtatt gtttgatttt aggaatttgg tccggtccgc ctgtaatgac
    20521 aatcgcctgt ggcgagcgtg ctaacacctg ttcgcggcta acttgcggcc agggaacccg
    20581 gctgtcttta aagatgtttt ctccgccaca aacttcgagt acctggttct gaatcgactc
    20641 ttttccactg gtaaatggcg gattaatgcc gaattgcaga aaaacacgtt ttttaggttt
    20701 atcagcatat tgcgctttca attgcacgta ctgatccagc agggattgcg cggcttgttc
    20761 ggccttgtct ggttgcggac tccagggggc cagttgacgt aacgcattgg caatttgttc
    20821 aatgcttgtc gcatcgaccc acatcacttt tattcccagc gaagccagct ggtcaacctg
    20881 ccgctcggca ttacctccac gccaggcaat caccagatcg ggtttcagcg cgacaatgcg
    20941 ttccagattc atcccctgcc aggtggaaac ctgctcaatc ttttgcgctt gtggaggata
    21001 gtcggaatag ctgctgaccc caaccggcgt gatcccggcg gcaaaggcaa gttcagtgtt
    21061 ggcgggagaa agcgtgatga cgcgcggcgc ggcgttgagc cacagtggcg cgagaaaaga
    21121 cagggcgacc agcgccctga acagtgattt agccatgtgc cagtttctgc accagtgact
    21181 caaccatcag gctggactgt ttagcggcaa cagccaggaa ctcatcgaag ctaagatgag
    21241 actgttgatc ggccacgtcg gagatggcgc gtacgacaac aaacgggacg ttgaaattgt
    21301 ggcagacatg ggcgattgcc gtcgcttcca tctctacagc aatggcctgt gggaagttgt
    21361 ggcggatttt cgccagacca acagaaccgt tgatgaaagc gtcgccgcta acaatcaggc
    21421 cacgtacagc gttaagattc agttcggcaa tgcaggcctc agcggcagcg atcagtttat
    21481 cgtcagcttt aaagcctgtc ggacagcctg gtaactgacc gtattcataa ccaaatgccg
    21541 tgacatccgc gtcgtgataa cgcgcttcgt ccgagacaac gatatcgccc actttcagcg
    21601 ttggtgccag gccaccggca gaaccggtgt taataatcac atctggcttg cagtgttcca
    21661 acagcagagt ggcacccagc gccgcagcga ctttaccgat gcccgatttc agaagcgcaa
    21721 cctcggttcc attcagttgg ccggtataga tttcgcaacc gccgagactg atagtttgac
    21781 ggttttcgat tttgtcacgc agcagcgtaa cttcttcttc cattgcacca atgatgccga
    21841 ttttcataga tttactcgcg ataagcccga tttgaaggca tagtttacca tgcgcttacg
    21901 gggaagcgta tttctcacgc gggagaggac atggcacaga ttgatttccg aaaaaaaata
    21961 aactggcatc gtcgttaccg ttcaccgcag ggcgttaaaa ccgaacatga gatcctgcgg
    22021 atcttcgaga gcgatcgcgg gcgtatcatc aactctccgg caattcgtcg tctgcaacaa
    22081 aagacccagg tttttccact ggagcgcaat gccgccgtgc gcacgcgtct tacccactcg
    22141 atggaagtcc agcaggtggg acgctacatc gccaaagaaa ttttaagccg tctgaaagag
    22201 cttaaattgc tggaagcata cggcctggat gaactgaccg gcccctttga aagcattgtt
    22261 gagatgtcat gcctgatgca cgatatcggc aatccgccgt ttggtcattt tggcgaagca
    22321 gcgataaatg actggtttcg ccaacgtttg cacccggaag atgccgaaag ccagcctctg
    22381 actgacgatc gctgcagcgt ggcggcacta cgtttacggg acggggaaga accgcttaac
    22441 gagctgcggc gtaagattcg tcaggactta tgtcattttg aggggaatgc acaaggcatt
    22501 cgtctggtgc atacattgat gcggatgaat ctcacttggg cacaggttgg cggtatttta
    22561 aaatataccc gtccggcgtg gtggcgtggc gaaacgcctg agacacatca ctatttaatg
    22621 aaaaagccgg gttattatct ttctgaagaa gcctatattg cccggttgcg taaagaactt
    22681 aatttggcgc tttacagtcg ttttccatta acgtggatta tggaagctgc cgacgacatc
    22741 tcctattgtg tggcagacct tgaagatgcg gtagagaaaa gaatatttac cgttgagcag
    22801 ctttatcatc atttgcacga agcgtggggc cagcatgaga aaggttcgct cttttcgctg
    22861 gtggttgaaa atgcctggga aaaatcacgc tcaaatagtt taagccgcag tacggaagat
    22921 cagtttttta tgtatttacg ggtaaacacc ctaaataaac tggtacccta cgcggcacaa
    22981 cgatttattg ataatctgcc tgcgattttc gccggaacgt ttaatcatgc attattggaa
    23041 gatgccagcg aatgcagcga tcttcttaag ctatataaaa atgtcgctgt aaaacatgta
    23101 tttagccatc cggatgtcga gcagcttgaa ttgcagggct atcgggtcat tagcggatta
    23161 ttagagattt atcgtccttt attaagcctg tcgttatcag actttactga actggtagaa
    23221 aaagaacggg tgaaacgttt ccctattgag tcgcgcttat tccacaaact ctcgacgcgc
    23281 catcggctgg cctatgtcga ggctgtcagt aaattaccgt cagattctcc tgagtttccg
    23341 ctatgggaat attattaccg ttgccgcctg ctgcaggatt atatcagcgg tatgaccgac
    23401 ctctatgcgt gggatgaata ccgacgtctg atggccgtag aacaataacc aggcttttgt
    23461 aaagacgaac aataaatttt taccttttgc agaaacttta gttcggaact tcgggctata
    23521 aaacgaatct gaagaacaca gcaattttgc gttatctgtt aatcgagact gaaatacatg
    23581 aaaaaaacca cattagcact gagtgcactg gctctgagtt taggtttggc gttatctccg
    23641 ctctctgcaa cggcggctga gacttcttca gcaacgacag cccagcagat gccaagcctt
    23701 gcaccgatgc tcgaaaaggt gatgccttca gtggtcagca ttaacgtaga aggtagcaca
    23761 accgttaata cgccgcgtat gccgcgtaat ttccagcagt tcttcggtga tgattctccg
    23821 ttctgccagg aaggttctcc gttccagagc tctccgttct gccagggggg ccagggcggt
    23881 aatggcggcg gccagcaaca gaaattcatg gcgctgggtt ccggcgtcat cattgatgcc
    23941 gataaaggct atgtcgtcac caacaaccac gttgttgata acgcgacggt gattaaagtc
    24001 cagctgagcg atggtcgtaa gttcgacgcg aagatggttg gcaaagatcc gcgctctgat
    24061 atcgcgctga tccaaatcca gaacccgaaa aaactgaccg caattaagat ggcggattct
    24121 gatgcactgc gcgtgggtga ttacaccgta gcgattggta acccgtttgg tctgggcgag
    24181 acggtaactt ccgggattgt ctctgcgctg gggcgtagcg gcctgaatgc tgaaaactac
    24241 gaaaacttca tccagaccga tgcagcgatc aaccgtggta actccggtgg tgcgctggtt
    24301 aacctgaacg gcgaactgat cggtatcaac accgcgatcc tcgcaccgga cggcggcaac
    24361 atcggtatcg gttttgctat cccgagtaac atggtgaaaa acctgacctc gcagatggtg
    24421 gaatacgggc aggtgaaacg cggtgagctg ggtattatgg ggactgagct gaattccgaa
    24481 ctggcgaaag cgatgaaagt tgacgcccag cgcggtgctt tcgtaagcca ggttctgcct
    24541 aattcctcgg ctgcaaaagc gggcattaaa gcgggtgatg tgatcacctc actgaacggt
    24601 aagccgatca gcagctttgc cgcactgcgt gctcaggtgg gtactatgcc ggtgggcagc
    24661 aaactgaccc tgggcttact gcgcgacggt aagcaggtta acgtgaacct ggaactgcag
    24721 cagagcagcc agaatcaggt tgattccagc tccatcttca acggcattga aggcgctgag
    24781 atgagcaaca aaggtaaaga tcagggcgtg gtagtgaaca acgtgaagac gggcactccg
    24841 gctgcgcaga tcggcctgaa gaaaggtgat gtgattattg gtgcgaacca gcaggcagtg
    24901 aaaaacatcg ctgaactgcg taaagttctc gacagcaaac cgtctgtgct ggcactgaac
    24961 attcagcgcg gcgacagcac catctacctg ttaatgcagt aatctccctc aaccccttcc
    25021 tgaatacggg aaggggttct ccttacaatc tgtgaacttc gccacaactc catacatctt
    25081 catcatcctt tgggcatttg cacaatgccg tacgttacgt acttccttat gctaagccgt
    25141 gcataacgga ggacttatgg ctggctggca tcttgatacc aaaatggcgc aggatatcgt
    25201 ggcacgtacc atgcgcatca tcgataccaa tatcaacgta atggatgccc gcgggcgaat
    25261 tatcggcagc ggcgatcgtg agcgtattgg tgaattgcac gaaggtgcat tgctggtact
    25321 ttcacaggga cgagtcgtcg atatcgatga cgcggtagca cgtcatctgc acggtgtgcg
    25381 gcaggggatt aatctaccgt tacggctgga aggtgaaatt gtcggcgtaa ttggcctgac
    25441 aggtgaacca gagaatctgc gtaaatatgg cgaactggtc tgcatgacgg ctgaaatgat
    25501 gctggaacag tcgcggttga tgcacttgtt ggcgcaggat agccgtttgc gggaagaact
    25561 ggtgatgaac ctgattcagg cagaggagaa tactcccgca cttctgaatg ggcgcaacgg
    25621 ctggggatcg atctcaatca accgcgagtg gtggctattg ttgaggtcga cagcggtcag
    25681 cttggcgtgg acagcgcaat ggcggagtta caacaactgc aaaacgcgct gactacgccc
    25741 gagcgtaata atctggtggc gattgtctcg ctaaccgaaa tggtggtgtt gaaaccggcg
    25801 ttgaactctt ttgggcgctg ggatgcagaa gatcatcgta agcgagttga acaactgatt
    25861 acccgcatga aagagtacgg ccagctgcgt tttcgcgttt cactgggcaa ctattttacc
    25921 ggtcctagca gtattgcccg atcctatcgt acggcgaaaa cgacgatggt ggtgggtaaa
    25981 cagcggatgc cagaaagtcg ctgctatttt tatcaggatc tgatgttacc tgtgttactc
    26041 gacagtttgc gtggcgactg gcaggccaac gaactggcgc gaccgctggc gcggctgaaa
    26101 gcgatggaca ataacggctt gctgcgacga acgctggcgg cgtggtttcg ccacaatgtg
    26161 caaccgctgg caacgtcaaa ggcgttgttt attcatcgta ataccctgga gtatcggctt
    26221 aatcgtatat cggaactgac cgggcttgat ttgggcaatt ttgatgacag gttgctgctg
    26281 tatgtggcgt tacaactgga tgaagagcgg tagattatgc gttaaggttg aggcggtgct
    26341 ggctcattcc cctcagcccg aaggggagag gggaatgccc gagttaaccg tcaattcggg
    26401 attatttacg cgttagtttt tccagatcgg cttcaatctc gctgatctta ttggttacga
    26461 ctgactccag gtgacgtaag tcgtcgagga tcttacgctt aagatcaact tcgctgcggt
    26521 cacgctgaca gatttgatca agctcatcaa tgatataccg tagattcggg ctgatttcct
    26581 ggacttcttt ataaccctga cccacaccat cagcgacgac cgttttacgc tgacgcggat
    26641 atttaaactt aacgctcttg gcgaaaaact cgcctttgtc tttctggaaa tagattttca
    26701 gaatatcgtt gttggcttcc tgccggaggc tgtaacgatc aatttcttca ggattggtaa
    26761 tacccagact tttcagattg tcgtacatag cgttacctca aaatgagtca gtaaattgtg
    26821 cttattttag catttggctt ggcccgcccg gctcgactgt gatcgcagaa agcctttttc
    26881 ttccagactt ctatcatggc gtaaaaatca aaaaattacc tgctttattg tggcgatatt
    26941 ccatgggatg gattagcaca tttatcttcg ttttgcgaat tacatgacag tccgaaacaa
    27001 gatgaaaaag tatttaaaaa agggggagga agaatgagta ggacgaatta ttctgagcca
    27061 gaaagcatac gggcagcatc atgctgcccg tatatgtact cttaatcgat ggtacgcagc
    27121 agttcgttaa tgccgacttt gccgcgagtt ttcgcgtcaa ctttcttaac gataatcgca
    27181 cagtagaggc tgtatttgcc atcttttgac ggcagattac ctgaaacaac cacagacccc
    27241 gccggaacgc gaccgtagtg gatttcgccg gtttcacggt cgtaaatacg ggtgctctga
    27301 ccaatgtata cgcccataga gatgacggaa ccttcttcga caatcacccc ttcaaccact
    27361 tcagagcgtg cgccaatgaa gcagttatct tcaataatgg tcgggttagc ctgcagcggc
    27421 tccagcacgc cgccgatgcc cacgccaccg gaaaggtgga cgtttttacc aatctgcgca
    27481 caagaaccga cggtcgccca ggtatcaacc atggtgcctt catcaacata tgcgccgatg
    27541 ttaacgtaag acggcatcag cacggtgtta cgggcaataa acgcaccctg acgtaccgcc
    27601 gctggtggca caacgcggaa gccttctttc tggaaacgtg cttcgtcgta gtcggcgaat
    27661 ttcatcggca ctttgtcgaa gtagcggctt tctgcccctt cgatcacctg attatcatta
    27721 atacggaaag agagcagcac cgcttttttc aaccactgat gcgtcaccca ctgaccgtca
    27781 attttttccg ctacacgcag tgcgccggaa tccagcaggg cgatcacctg atttaccgct
    27841 tcgcgggtaa cggtgtctgc attggctggc gtgatatcgg cacggcgttc aaaagcggtt
    27901 tcaataatgt tctgtaactg ctgcattgtt aaactctttt catatcagta aacacatcac
    27961 cctttatcgt ttggattgag ggcctctgtc aaccgctgat gcacttcctg ctgcaactcg
    28021 ttattaagcg cacgccggtc agcggtggca attatgaata aatcttctac tcgctcgcca
    28081 atggttgtaa ttcgggcacc atgaagcgaa attcccagat cggcaaaaat tttcccgact
    28141 cgcgccagca gtccaggttg gtcgagggcg atcagttcga ggaacgattt gcggtcggta
    28201 tgggtcggca aaaacgttac ttcggtttca acagtaaaat ggcgtaattt ggcgggttgg
    28261 cgacggggct gcggtggctg ccagctactt tgcgtcagca cctgctccag accaaaccga
    28321 ataacctcat ggcgatctgc ggacagcggg ctgccatcgg gttccagcac gataaaggta
    28381 tccatcgcca taccgtcgcg agtggtgaaa atttgtgcgt cgtgaacact taaattgcgg
    28441 cggtctaatt cggcacagac ggcggcaaac agataggggc ggtccgggct ccagataaaa
    28501 atctcggtgc ctccacgcgt agcctgcggg ctaagcaata ccagcggttt gcttaaatca
    28561 tgctgtaata aatggcgggc atgccaggcc agttgatttg ggctatggcg gacaaaatag
    28621 ttagcacgac agcgtgacca aatttggtgc agcgcctctt cgtcgatgtt atccatgcgc
    28681 agtagtgcca gtgcctggag ttggtgatgg cgaacccgtt cgcgcatatc cggcgtgttt
    28741 tgcattccgc gtcgtagctg cttttcggtg gcaaaataga gttcgcgcaa cagactttgc
    28801 ttccagctat tccacagcgt ttcgttggtg gcgcaaatgt cagccactgt caggcatacc
    28861 agatagcgca gacgattttc cgtttgaact tcttcggcaa actgcttgat gacttcaggg
    28921 tcctgaatat cgcggcgttg tgctgtcacc gacatcagca agtgctggcg aaccagccag
    28981 gcaaccagct gtgtttcgcg tgagttcagc ccgtggagtt ctgcaaaatg cactacatcc
    29041 tgagcaccga gaatggagtg gtcgccgccg cgtcctttgg cgatatcgtg aaacagcgcg
    29101 gcgatgaaaa tcagctcagt tgacggcagg cgcggccaga cgtccacaca caacggatgg
    29161 cgctggcgcg tttcttcact ggcaaaactc tccagtttca gcatcacgcg gatagtatgt
    29221 tcatccaccg tgtaggcgtg gaacagatca aactgcatct gcccgacgat atgcgaccat
    29281 tgcggcatat acgcgccgag cacgctatgg cgatgcattg gcaatagccc gcgccgcacc
    29341 gctccggggt gacgcagaat gctcaaaaac agttttcgtg cttgcggaat attacacagc
    29401 ggttgttgca gatggcgacg ggcatggcgt aactggcgca gcgtggtgga gtaaataccg
    29461 gtgatcgcac tgttgcgcac catggtgtag aacatacgca agatggcttc cggctggcgc
    29521 ataaatagtg tttcatcacg caggtcgatt agcgtaccgc gtagctgaaa ctcatcgtcg
    29581 agtggacgtg gtttttcgtc ggcgggaagg gcgaggatgg cttcatcgaa cagttgcagc
    29641 agcatctggt tgagttcact gacgcggcgt gtaacgcgga agtaatcctt catcatccgc
    29701 tcgaccggtt cgttgccttc accactgtaa ttcagacgct gagcgacgct aagttggcga
    29761 tcgaataaca gacgattatc gtaacggctg acgaccagat gcagggcaaa gcgaatatgc
    29821 cacagtatat gcagacattc gtttaattcc gcccgctccg ctgaggttaa gaagccaaac
    29881 ccgaccattt catccagcga tgttgcgcca aaatgacggc gggccaccca ttgcagagtg
    29941 tgaatatcgc gcaagccgcc agggctactt ttgatgtctg gttcaaggtt gtagctggtg
    30001 ccatggtaac gctgatggcg ctggttctgt tcttcaactt tcgctgcgta gaacttgtcg
    30061 gaaggccaga atccttcgct aaaaatatgt ttttgcagtt cgaggaagag cgcaacatcg
    30121 ccaattaata agcgggattc gattaaattg gtggcgacgg ttaaatccga taacccttcc
    30181 aacatgcact cttcaagcgt gcgcacacta tgaccgactt ccagctttac atcccagagc
    30241 agcgttaaca gctcgcccac tttttgcgcc tgatcgtccg ggagcttttt acggcttaaa
    30301 atcagtaaat cgatgtcaga aagtggatgc agctcgccac gaccgtagcc accgacggca
    30361 accaatgcca ggtcggcaat ctggctgaat cccgcttcaa tccataatcg ttgcaggagc
    30421 tggtctatga actcggtacg tgcctcaatc aactgttctg cagagatccc attgtcaaag
    30481 gcatcaccca gccaacgctg gaaagtatcg atatgggctt ttatcccaca gacggttaat
    30541 tcatcacggg gccaggcgca tggattttgc ggttgaccgg gcagggtgga gagagcggtg
    30601 tttgcgtact gttctggaag ggtattcatt gtgcgccacc cataagatta aattatcgca
    30661 ttaaaaaagc cggcattcgc cggcttcatc ttattcgtcg tgcgagatta tcgccgggat
    30721 ggtgtcatcc ttgcgtagcg tcaaaatttc gcagccgtta tcagtcacca caatagtatg
    30781 ctcatattgt gcagacaagc tgcgatcttt ggtttttacc gtccagccat ctttcatggt
    30841 gcggatctct tttttacccg cgttgaccat tggctcaatg gtgaacgtca tccccggttt
    30901 cagcacgacg ttggtttcac gggagtcata gtgcagcacc tgcggttctt catggaagcc
    30961 gcgaccaata ccgtgtccgc aatattcacg aacgacggag aagccttctg cttcgacaaa
    31021 tttctgaatc gccgcaccga tttcgcgcag attaatgcct ggttttacca tgcgtagcgc
    31081 caggtacagg ctttcttgcg tgatgcggca caggcgttcg cccatgatgg tcggcttacc
    31141 gacgataaac attttcgagg tatcgccgtg gaaaccatct ttgattacgg tgacatcaat
    31201 gttaacgata tcgccatctt tcagcagctt agcatcgtcc gggataccgt ggcacaccac
    31261 ttcattaata gagatgcaaa cggatttcgg atagccgtga tagccgaggc aggcagaaac
    31321 cgcgtgttgt tcattaacaa tgtaatcatt acagatgcga tccagctcgc cggtgctgac
    31381 gcccggttta acatacggtt cgatcatctc cagcacttcg gcagccagtc ggccagcgac
    31441 gcgcattttt tcgatatctt ctggggtctt gattgagata gccattaatt ctgtccatca
    31501 gcgtcggtga taccgacaat atatatgtaa gtgccgtcaa tggtatcaca cccgggcaaa
    31561 ttgagaatca ttctgaattt cgccaaacgt gccactgaag gttttctata atagaaaatt
    31621 cgacgtctga tgctgtacac agcgccaaca attattggtg tccacgacgt atttgtggta
    31681 taaagcgcgc cggacttccg atccatttcg tatacacaga ctggacggaa gcgacaatct
    31741 cactttgtgt aacaacacac acgtatcggc acatattccg gggtgccctt tggggtcggt
    31801 aatatgggat acgtggaggc ataaccccaa cttttatata gaggttttaa tcatggcaac
    31861 tgtttccatg cgcgacatgc tcaaggctgg tgttcacttc ggtcaccaga cccgttactg
    31921 gaacccgaaa atgaagccgt tcatcttcgg tgcgcgtaac aaagttcaca tcatcaacct
    31981 tgagaaaact gtaccgatgt tcaacgaagc tctggctgaa ctgaacaaga ttgtttctcg
    32041 caaaggtaaa atccttttcg ttggtactaa acgcgctgca agcgaagcgg tgaaagacgc
    32101 tgctctgagc tgcgaccagt tcttcgtgaa ccatcgctgg ctgggcggta tgctgactaa
    32161 ctggaaaacc gttcgtcagt ccatcaaacg tctgaaagac ctggaaactc agtctcagga
    32221 cggtactttc gacaagctga ccaagaaaga agcgctgatg cgcactcgtg agctggagaa
    32281 actggaaaac agcctgggcg gtatcaaaga catgggcggt ctgccggacg ctctgtttgt
    32341 aatcgatgct gaccacgaac acattgctat caaagaagca aacaacttgg gtattccggt
    32401 atttgctatc gttgatacca actctgatcc ggacggtgtt gacttcgtta tcccgggtaa
    32461 cgacgacgca atccgtgctg tgaccctgta cctgggcgct gttgctgcaa ccgtacgtga
    32521 aggccgttct caggatctgg cttcccaggc ggaagaaagc ttcgtagaag ctgagtaata
    32581 aggcttgata actcccccaa aatagttcga gttgcagaaa ggcggcaagc tcgagaattc
    32641 ccgggagctt acatcagtaa gtgaccggga tgagcgagcg aagacaacgc atctgcggcg
    32701 cgaaatatga agggggagag cccttataga ccaggtagta cacgtttggt tagggggcct
    32761 gcatatggcc ccctttttca cttttatatc tgtgcggttt aatgccgggc agatcacatc
    32821 tccgaggatt ttagaatggc tgaaattacc gcatccctgg taaaagagct gcgtgagcgt
    32881 actggcgcag gcatgatgga ttgcaaaaaa gcactgactg aagctaacgg cgacatcgag
    32941 ctggcaatcg aaaacatgcg taagtccggt gctattaaag cagcgaaaaa agcaggcaac
    33001 gttgctgctg acggcgtgat caaaaccaaa atcgacggca actacggcat cattctggaa
    33061 gttaactgcc agactgactt cgttgcaaaa gacgctggtt tccaggcgtt cgcagacaaa
    33121 gttctggacg cagctgttgc tggcaaaatc actgacgttg aagttctgaa agcacagttc
    33181 gaagaagaac gtgttgcgct ggtagcgaaa attggtgaaa acatcaacat tcgccgcgtt
    33241 gctgcgctgg aaggcgacgt tctgggttct tatcagcacg gtgcgcgtat cggcgttctg
    33301 gttgctgcta aaggcgctga cgaagagctg gttaaacaca tcgctatgca cgttgctgca
    33361 agcaagccag aattcatcaa accagaagac gtatccgctg aagtggtaga aaaagaatac
    33421 caggtacagc tggatatcgc gatgcagtct ggtaagccga aagaaatcgc agagaaaatg
    33481 gttgaaggcc gcatgaagaa attcaccggc gaagtttctc tgaccggtca gccgttcgtt
    33541 atggaaccaa gcaaaactgt tggtcagctg ctgaaagagc ataacgctga agtgactggc
    33601 ttcatccgtt tcgaagtggg tgaaggcatc gagaaagttg agactgactt tgcagcagaa
    33661 gttgctgcga tgtccaagca gtcttaatta tcaaaaagga gccgcctgag ggcggcttct
    33721 ttttgtgccc atcttgtaaa ttcagctaac ccttgtgggg ctgcgctgaa aagcgacgta
    33781 caatgtcgct agtattaatt catttcaatc gttgacagtc tcaggaaaga aacatggcta
    33841 ccaatgcaaa acccgtctat aaacgcattc tgcttaagtt gagtggcgaa gctctgcagg
    33901 gcactgaagg cttcggtatt gatgcaagca tactggatcg tatggctcag gaaatcaaag
    33961 aactggttga actgggtatt caggttggtg tggtgattgg tgggggtaac ctgttccgtg
    34021 gcgctggtct ggcgaaagcg ggtatgaacc gcgttgtggg cgaccacatg gggatgctgg
    34081 cgaccgtaat gaacggcttg gcaatgcgtg atgcactgca ccgcgcctat gtgaacgctc
    34141 gtctgatgtc cgctattcca ttgaatggcg tgtgcgacag ctacagctgg gcagaagcta
    34201 tcagcctgtt gcgcaacaac cgtgtggtga tcctctccgc cggtacaggt aacccgttct
    34261 ttaccaccga ctcagcagct tgcctgcgtg gtatcgaaat tgaagccgat gtggtgctga
    34321 aagcaaccaa agttgacggc gtgtttaccg ctgatccggc gaaagatcca accgcaacca
    34381 tgtacgagca attgacttac agcgaagtgc tggaaaaaga gctgaaagtc atggacctgg
    34441 cggccttcac gctggctcgt gaccataaat taccgattcg tgttttcaat atgaacaaac
    34501 cgggtgcgct gcgccgtgtg gtaatgggtg aaaaagaagg gactttaatc acggaataat
    34561 tcccgtgatg gataaataag ggtaagattc cgcgtaagta tcgcgggggc gtaagtctgg
    34621 ttataaggcg ttattgttgc aggcagtttg gtcacggcca gcgcgcagca accggagcgt
    34681 acaaaagtac gtgaggatgg cgagcactgc tcggggccaa aatggcaaat aaaatagcct
    34741 aataatccag acgattaccc gtaatatgtt taatcagggc tatacttagc acacttccac
    34801 tgtgtgtgac tgtctggtct gactgagaca agttttcaag gattcgtaac gtgattagcg
    34861 atatcagaaa agatgctgaa gtacgcatgg acaaatgcgt agaagcgttc aaaacccaaa
    34921 tcagcaaaat acgcacgggt cgtgcttctc ccagcctgct ggatggcatt gtcgtggaat
    34981 attacggcac gccgacgccg ctgcgtcagc tggcaagcgt aacggtagaa gattcccgta
    35041 ctctgaaaat caacgtgttt gatcgttcaa tgtctccggc cgttgaaaaa gcgattatgg
    35101 cctccgatct cggcctgaac ccgaactctg cgggtagcga catccgtgtt ccgctgccgc
    35161 cgctgacgga agaacgtcgt aaagatctga ccaaaatcgt tcgtggtgaa gcagaacaag
    35221 cgcgtgttgc agtacgtaat gtgcgtcgtg acgcgaacga caaagtgaaa gcactgttga
    35281 aagataaaga gatcagcgaa gacgacgatc gccgttctca ggacgatgta cagaaactga
    35341 ctgatgctgc aatcaagaaa attgaagcgg cgctggcaga caaagaagca gaactgatgc
    35401 agttctgatt tcttgaacga caaaaacgcc gctcagtaga tccttgcgga tcggctggcg
    35461 gcgttttgct ttttattctg tctcaactct ggatgtttca tgaagcaact caccattctg
    35521 ggctcgaccg gctcgattgg ttgcagcacg ctggacgtgg tgcgccataa tcccgaacac
    35581 ttccgcgtag ttgcgctggt ggcaggcaaa aatgtcactc gcatggtaga acagtgcctg
    35641 gaattctctc cccgctatgc cgtaatggac gatgaagcga gtgcgaaact tcttaaaacg
    35701 atgctacagc aacaggggag ccgcaccgaa gtcttaagtg ggcaacaagc cgcttgcgat
    35761 atggcagcgc ttgaggaggt tgatcaggtg atggcagcca ttgttggtgc tgctgggctg
    35821 ttacctacgc ttgctgcgat ccgcgcgggt aaaaccattt tgctggccaa taaagaatca
    35881 ctggttacct gcggacgtct gtttatggac gccgtaaagc agagcaaagc gcaattgtta
    35941 ccggtcgata gcgaacataa cgctattttt cagagtttac cgcaacctat ccagcataat
    36001 ctgggatacg ctgaccttga gcaaaatggc gtggtgtcca ttttacttac cgggtctggt
    36061 ggccctttcc gtgagacgcc attgcgcgat ttggcaacaa tgacgcccga tcaagcttgc
    36121 cgtcatccga actggtcgat gggacgtaaa atttctgtcg attcggctac catgatgaac
    36181 aaaggtctgg aatacattga agcgcgttgg ctgtttaacg ccagcgccag ccagatggaa
    36241 gtgctgattc acccgcagtc agtgattcac tcaatggtgc gctatcagga cggcagtgtt
    36301 ctggcgcagc tgggggaacc ggatatgcgt acgccaattg cccacaccat ggcatggccg
    36361 aatcgcgtga actctggcgt gaagccgctc gatttttgca aactaagtgc gttgacattt
    36421 gccgcaccgg attatgagcg ttatccatgc ctgaaactgg cgatggaggc gttcgaacaa
    36481 ggccaggcag cgacgacagc attgaatgcc gcaaacgaaa tcaccgttgc tgcttttctt
    36541 gcgcaacaaa tccgctttac ggatatcgct gcgttgaatt tatccgtact ggaaaaaatg
    36601 gatatgcgcg aaccacaatg tgtggacgat gtgttatctg ttgatgcgaa cgcgagtgaa
    36661 gtcgccagaa aagaggtgat gcgtctcgca agctgaggat aatccggcta cagagagtcg
    36721 cgctatttgt tagcgtaggg cttcaatgat atagtctgcg ccatctgatc gtaagtagtt
    36781 ggctttataa ggtcaggtat gccgtggttt tacacggctt ttttttgtat aggcttcagt
    36841 attcctgagt accgtaaacc ctgtcaggga ataaaaaacg cgtgatgttg tctgctactc
    36901 aaccacttag cgaaaaattg ccagcgcatg gctgccgtca tgttgcgatc attatggacg
    36961 gcaatggtcg ctgggcaaaa aagcaaggga agattcgtgc ctttgggcat aaagccgggg
    37021 caaaatccgt ccgccgggct gtctcttttg cggccaacaa cggtattgaa gcgttaacgc
    37081 tgtatgcctt tagtagtgaa aactggaacc gaccagcgca ggaagtcagt gcgttaatgg
    37141 aactgtttgt gtgggcgctc gatagcgaag taaaaagtct gcaccgacat aacgtgcgtc
    37201 tgcgtattat tggcgatacc agtcgtttta actcgcgttt gcaagaacgt attcgtaaat
    37261 ctgaagcgct aacagccgga aataccggtc tgacgctgaa tattgcggcg aactacggtg
    37321 gacgttggga tatagtccag ggagtcaggc aactggctga aaaggtgcag caaggaaacc
    37381 tgcaaccaga tcagatagat gaagagatgt taaaccagca tgtctgtatg catgaactgg
    37441 cccctgtaga tttagtaatt aggactgggg gagagcatcg cattagtaac tttttgcttt
    37501 ggcaaattgc ctatgccgaa ctttacttta cagatgttct ctggcccgat ttcgatgaac
    37561 aagactttga aggggcgtta aatgcctttg ctaatcgaga gcgtcgtttc ggcggcaccg
    37621 agcccggtga tgaaacagcc tgatgggggt cgcttttgct gaagtatcgc ctgatatctg
    37681 cttttgtgtt aatacccgtc gtcatcgcgg cgttgtttct gttgccgccg gtggggttcg
    37741 ccattgtaac gctggtggtc tgcatgctgg cagcgtggga atggggacag cttagcggtt
    37801 ttaccactcg ttcgcagcga gtatggttgg cggtgttatg cgggttattg ttggcgctga
    37861 tgctttttct gttgccggaa tatcaccgaa atattcatca accgctggtt gaaatctcac
    37921 tttgggcttc gttgggttgg tggattgtcg cgctattgct ggtgctgttt tacccaggtt
    37981 ccgcagcaat ctggcgtaac tctaaaacat tgcgccttat ttttggcgtg ctaaccattg
    38041 ttcccttctt ctggggcatg ctggcgttac gggcctggca ctatgacgag aatcattaca
    38101 gtggcgcaat atggctgctt tatgtcatga tcctggtatg gggcgctgac tccggcgcat
    38161 atatgtttgg caaattgttt ggtaaacata agctggcacc gaaggtttct ccgggtaaaa
    38221 cctggcaagg ctttataggt ggactcgcta ctgcagcggt aatctcatgg ggttatggca
    38281 tgtgggcgaa tctcgacgtc gctcccgtca ccttactcat ttgctctatt gtcgcagcgt
    38341 tagcctcagt gctcggcgat ctgaccgaga gtatgtttaa gcgtgaagca ggaattaagg
    38401 acagcggtca tttaattcca ggacacggtg gtattttaga tcgtattgat agcctgacgg
    38461 ctgcggtacc ggtctttgct tgcttgttgt tactggtatt caggacgctt taacggaagg
    38521 taatatgctg agttttctct gggatttggc ttcgttcatc gttgcactgg gtgtacttat
    38581 caccgtgcat gaatttggtc atttctgggt tgcccggcgt tgtggtgttc gcgttgagcg
    38641 tttctcaata gggtttggta aggcgctctg gcggcgaact gataagctcg gcaccgaata
    38701 tgttatcgcc ctgatcccgt tgggcggtta tgtcaaaatg ctggatgagc gcgcagaacc
    38761 ggtcgttccg gaactccgcc accatgcctt caataataaa tctgtcggcc aacgagtggc
    38821 gattattgcc gcaggtccgg ttgcaaactt catttttgct atctttgcct actggctggt
    38881 ttttattatt ggtgtgcctg gcgtacgtcc ggtggttggc gaaatagcag ccaattcgat
    38941 agctgcggaa gcacaaattg caccaggtac ggaactaaaa gccgtagatg gtatcgaaac
    39001 gcctgattgg gatgccgtgc gtttgcagtt ggtcgataaa attggcgatg aaagcaccac
    39061 cattacagta gcgccatttg gcagcgacca acggcgggat gtaaagctcg atttacgtca
    39121 ctgggcgttt gagcctgata aagaagatcc ggtatcttcg ctggggattc gtcctcgtgg
    39181 gccgcaaatt gaacctgtac tggaaaatgt gcagccaaac tcggcggcaa gcaaggcagg
    39241 tttgcaagca ggcgacagga tcgttaaagt cgatggtcag cccttaacgc agtgggtgac
    39301 ctttgtgatg cttgtccggg ataacccggg taaatcctta gcgttagaaa tcgaaaggca
    39361 ggggagtccc ttgtctttga cattaatccc ggagagtaaa ccgggtaatg gtaaagcgat
    39421 tggttttgtc ggtattgagc cgaaagtcat tcctttgcca gatgagtata aagttgtacg
    39481 ccagtatggg ccgttcaacg ccatcgtcga agccacggac aaaacgtggc agctgatgaa
    39541 gctgacggtc agtatgctgg gaaaattgat caccggtgat gtgaaactga acaacctcag
    39601 tgggccgatt tctatcgcca agggggctgg gatgacagcg gaactcgggg tagtttatta
    39661 cctgccgttt cttgcgctta ttagcgtgaa cttagggata attaacctgt ttccgttgcc
    39721 cgtacttgac ggggggcatc tgctgttcct tgcgatcgaa aagatcaagg gcggaccggt
    39781 atccgagcgg gttcaagact tttgttatcg cattggctcg attctgctgg tgctgttaat
    39841 ggggcttgca cttttcaatg atttctctcg gttatgagag ttagttagga agaacgcata
    39901 ataacgatgg cgatgaaaaa gttgctcata gcgtcgctgc tgtttagcag cgccaccgta
    39961 tacggtgctg aagggttcgt agtgaaagat attcatttcg aaggccttca gcgtgtcgcc
    40021 gttggtgcgg ccctcctcag tatgccggtg cgtacaggcg acacggttaa tgatgaagat
    40081 atcagtaata ccattcgcgc tctgtttgct accggcaact ttgaggatgt tcgcgtcctt
    40141 cgtgatggtg atacccttct ggttcaggta aaagaacgtc cgaccattgc cagcattact
    40201 ttctccggta acaaatcggt gaaagatgac atgctaaagc aaaacctcga ggcttctggt
    40261 gtgcgtgtgg gcgaatccct cgatcgcacc accattgccg atatcgagaa aggtctggaa
    40321 gacttctact acagcgtcgg taaatatagc gccagcgtta aagctgtcgt gaccccgctg
    40381 ccgcgcaacc gtgttgacct aaaactggtg ttccgggaag gtgtgtcagc tgaaatccag
    40441 caaattaaca ttgttggtaa ccatgctttc accaccgacg aactgatctc tcatttccaa
    40501 ctgcgtgacg aagtgccgtg gtggaatgtg gtaggcgatc gtaaatacca gaaacagaaa
    40561 ctggcgggcg accttgaaac cctgcgcagc tactatctgg accgcggtta tgcccgtttc
    40621 aacatcgact ctacccaggt cagtctgacg ccagataaaa aaggtattta cgtcacggtg
    40681 aacatcaccg aaggcgatca gtacaagctt tctggcgttg aagtgagcgg caaccttgcc
    40741 gggcactccg ctgaaattga gcagctgact aagatcgagc cgggcgagct gtataacggc
    40801 actaaagtga ccaaaatgga agatgacatt aaaaagcttc tcggtcgcta tggttatgcc
    40861 tatccgcgcg tacagtcgat gcctgaaatt aacgatgccg acaaaaccgt taaattacgc
    40921 gtgaacgttg atgcgggtaa ccgtttctac gtgcgtaaga tccgttttga aggtaacgat
    40981 acctcgaaag atgccgtcct gcgtcgcgaa atgcgtcaga tggaaggtgc atggttgggg
    41041 agcgatctgg tcgatcaggg taaggagcgt ctgaatcgtc tgggcttctt tgaaactgtc
    41101 gatactgata cccaacgtgt tccgggtagc ccggaccagg ttgatgtagt ctacaaggtg
    41161 aaagaacgta acactggtag cttcaacttc gggattggtt acggtactga aagtggcgtg
    41221 agcttccagg ctggcgtgca acaggataac tggttaggta caggttatgc tgttggtatc
    41281 aacgggacca aaaacgatta ccagacctat gctgaactgt cggtaaccaa cccgtacttc
    41341 accgtagatg gcgtaagcct cggtggtcgt ctcttctata atgacttcca ggcagatgac
    41401 gccgacctgt ccgactatac caacaagagt tatggtacag acgtgacgtt gggcttcccg
    41461 attaacgaat ataactcgct gcgtgcaggt ctgggttatg tacataactc cctgtccaac
    41521 atgcagcctc aggttgcgat gtggcgttat ctgtactcta tgggtgaaca tccgagcact
    41581 tctgatcagg ataacagctt caaaacggac gacttcacgt tcaactatgg ttggacctat
    41641 aacaagcttg accgtggtta cttcccgaca gatggttcac gtgtcaacct gaccggtaaa
    41701 gtgaccattc ctggatcgga taacgaatac tacaaagtga cgttagacac ggcgacttat
    41761 gtgccgatcg atgacgatca caaatgggtt gttctgggtc gtacccgctg gggttatggt
    41821 gatggtttag gcggcaaaga gatgccgttc tacgagaact tctatgccgg tggttccagc
    41881 accgtgcgtg gcttccagtc caataccatt ggtccgaaag cagtttactt cccgcatcag
    41941 gccagtaatt atgatccgga ctatgattac gaatgtgcga ctcaggacgg cgcgaaagac
    42001 ctgtgtaaat cggatgatgc tgtaggcggt aacgccatgg cggttgccag cctcgagttc
    42061 atcaccccga cgccgtttat tagcgataag tatgctaact cggttcgtac ttcattcttc
    42121 tgggatatgg gtaccgtttg ggatacaaac tgggattcca gccaatattc tggatatccg
    42181 gactatagtg atccaagcaa tatccgtatg tctgcgggta tcgcattaca atggatgtcc
    42241 ccattggggc cgttggtgtt ctcctacgcc cagccgttca aaaagtacga tggagacaag
    42301 gcagaacagt tccagtttaa catcggtaaa acctggtaag tgttctccac aaaggaatgt
    42361 agtggtagtg tagcgatgac tttaggcgat caatataaga tcgccgggcc acgcaaagaa
    42421 ctgcaccctc cggtgcaaat gggatggtaa ggagtttatt gtgaaaaagt ggttattagc
    42481 tgcaggtctt ggtttagcac tggcaacttc tgctcaggcg gctgacaaaa ttgcaatcgt
    42541 caatatgggc agcctgttcc agcaggtagc gcagaaaacc ggtgtttcta acacgctgga
    42601 aaatgagttc aaaggccgtg ccagcgaact gcagcgtatg gaaaccgatc tgcaggctaa
    42661 aatgaaaaaa ctgcagtcca tgaaagcggg cagcgatcgc accaaactgg aaaaagacgt
    42721 gatggctcag cgccagactt ttgctcagaa agcgcaggct tttgagcagg atcgcgcacg
    42781 tcgttccaac gaagaacgcg gcaaactggt tactcgtatc cagactgctg tgaaatccgt
    42841 tgccaacagc caggatatcg atctggttgt tgatgcaaac gctgttgctt acaacagcag
    42901 cgatgtaaaa gacatcactg ccgacgtact gaaacaggtt aaataagtaa tgccttcaat
    42961 tcgactggct gatttagcgc agcagttgga tgcagaacta cacggtgatg gcgatatcgt
    43021 catcaccggc gttgcgtcca tgcaatctgc acaaacaggt cacattacgt tcatggttaa
    43081 cccaaaatac cgtgagcatt taggcttgtg ccaagcgtcc gcggttgtca tgacccagga
    43141 cgatcttcct ttcgcgaaaa gtgccgcgct ggtagtgaag aatccctacc tgacttacgc
    43201 gcgcatggcg caaattttag ataccacgcc gcagcccgcg cagaacattg cacccagtgc
    43261 ggtgatcgac gcgacggcga agctgggtaa caacgtatcg attggcgcta acgcggtgat
    43321 tgagtccggc gttgaactgg gcgataacgt gattatcggt gccggttgct tcgtaggtaa
    43381 aaacagcaaa atcggtgcag gttcgcgtct ctgggcgaac gtaaccattt accatgagat
    43441 ccagatcggt cagaattgcc tgatccagtc cggaacagtg gtaggcgcag acggctttgg
    43501 ttatgccaac gatcgtggta actgggtgaa gatcccacag attggtcgcg taattattgg
    43561 cgatcgcgtg gagatcggtg cctgcacaac catcgatcgc ggcgcgctgg atgacactgt
    43621 tattggcaat ggcgtgatca ttgataacca gtgccagatt gcacataacg tcgtgattgg
    43681 cgacaatacg gcggttgccg gtggcgtcat tatggcgggc agcctgaaaa ttggtcgtta
    43741 ctgcatgatc ggcggagcca gcgtaatcaa cgggcatatg gaaatatgcg acaaagtgac
    43801 ggttacgggc atgggtatgg tgatgcgtcc catcactgaa ccaggcgtct attcctcagg
    43861 cattccgctg caacccaaca aagtctggcg caaaaccgct gcactggtga tgaacattga
    43921 tgacatgagc aagcgtctga aatcgcttga gcgcaaggtt aatcaacaag actaacgttc
    43981 catcttttgt tcgccaaact ttacggcctg tctcattctt acgattgcgg caggccgtgt
    44041 tattattgtc gtttcttata ttttgacagg aagagtatct tgactactaa cactcatact
    44101 ctgcagattg aagagatttt agaacttctg ccgcaccgtt tcccgttctt actggtggat
    44161 cgcgtgctgg attttgaaga aggtcgtttt ctgcgcgcag taaaaaatgt ctctgtcaat
    44221 gagccattct tccagggcca tttccctgga aaaccgattt tcccgggtgt gctgattctg
    44281 gaagcaatgg cacaggcaac aggtattctg gcgtttaaaa gcgtaggaaa actggaaccg
    44341 ggtgagctgt actacttcgc tggtattgac gaagcgcgct tcaagcgccc ggtcgtgcct
    44401 ggcgatcaaa tgatcatgga agtcactttc gaaaaaacgc gccgcggcct gacccgtttt
    44461 aaaggggttg ctctggtcga tggtaaagta gtttgcgaag caacgatgat gtgtgctcgt
    44521 agccgggagg cctgatacgt gattgataaa tccgcctttg tgcatccaac cgccattgtg
    44581 gaagagggcg cgtcaattgg cgcgaacgca cacattggtc ctttttgtat cgttggaccc
    44641 catgtcgaaa ttggtgaggg taccgtactg aaatctcacg ttgtcgtgaa tggtcatact
    44701 aaaattggcc gcgataatga gatttatcag tttgcctcca tcggcgaagt taaccaggat
    44761 ttgaaatatg ctggcgaacc gacccgtgtg gaaatcggcg atcgtaaccg cattcgcgaa
    44821 agcgtcacca ttcatcgtgg cacagtccag ggcggtggat tgacgaaggt gggcagcgac
    44881 aacttactga tgatcaacgc gcacattgcg cacgattgta cggtaggtaa ccgctgtatt
    44941 ctcgccaaca acgcaacgct ggcgggtcac gtatcggttg acgacttcgc gatcatcggc
    45001 ggcatgaccg cagtccatca gttctgcatc attggtgcgc atgtgatggt tggcggctgc
    45061 tccggtgtgg cgcaggacgt ccctccttat gtcattgcgc agggtaacca cgcaacgccg
    45121 ttcggtgtca atatcgaagg gctgaagcgc cgcggattca gccgtgaggc gattaccgct
    45181 atccgcaatg cgtataagct aatttatcgt agcggtaaaa cgctcgatga agtgaaaccg
    45241 gaaattgctg aactggcgga aacatatccg gaagtgaaag cctttaccga tttctttgca
    45301 cgctcaacgc gcggtctgat tcgttaatga ctgaccagcg tccattaacg attgccctgg
    45361 tcgccggaga aacctccggc gatatcctgg gggccggttt aatccgcgct ctgaaagaac
    45421 gtgtgcccaa cgcccgcttt gttggtgttg ccgggccacg aatgcaggct gaaggctgcg
    45481 aagcctggta cgaaatggaa gaactggcgg tgatgggcat tgttgaagtg ctcggtcgtc
    45541 tgcgtcgctt actgcatatt cgtgccgatc tgacaaagcg ttttggcgaa ctgaagccag
    45601 atgtttttgt tggtattgat gcgcccgact tcaatattac tcttgaaggt aacctcaaaa
    45661 agcagggtat caaaaccatt cattacgtca gtccatccgt ctgggcgtgg cgacagaaac
    45721 gcgttttcaa aataggcaga gccaccgatc tggtgctcgc atttctgcct ttcgaaaaag
    45781 cgttttatga caaatacaat gtaccgtgcc gctttatcgg tcataccatg gctgatgcca
    45841 tgccgttaga tccagataaa aatggtgccc gtgatgtgct ggggatccct tacgatgccc
    45901 actgtctggc attgttgccg ggcagccgtg gtgcagaagt cgaaatgctt agtgccgatt
    45961 tcttgaaaac tgcccagctt ttgcgccaga catatccgga tctcgaaatc gtggtgccgc
    46021 tggtgaatgc caaacgccgc gagcagtttg aacgcatcaa agctgaagtc gcgccagacc
    46081 tgtcggttca tttgctggat ggaatgggcc gtgaggcgat ggtcgccagc gatgcggcac
    46141 tactggcgtc ggggacggca gccctggagt gtatgctggc gaaatgcccg atggtggtgg
    46201 gatatcgcat gaagcctttt accttctggt tggcgaagcg gctggtgaaa actgattatg
    46261 tctcgctgcc aaatctgctg gcgggcagag agttagtcaa agagttattg caggaagagt
    46321 gtgagccgca aaaactggct gcggcgctgt taccgctgtt ggcgaacggg aaaaccagcc
    46381 acgcgatgca cgataccttc cgtgaactgc atcagcagat ccgctgcaat gccgatgagc
    46441 aggcggcaca agccgttctg gagttagcac aatgatcgaa tttgtttatc cgcacacgca
    46501 gctggttgcg ggtgtggatg aagtcggacg cgggccgtta gttggcgcgg tcgtcaccgc
    46561 tgcggtgatc cttgacccgg cgcgcccgat tgccgggctg aatgattcca aaaagctgag
    46621 cgaaaaacgc cgtctggcgc tctatgaaga gatcaaagag aaagcgttga gctggagtct
    46681 gggccgcgcg gaaccccacg aaatcgacga gctgaacatt cttcatgcga ccatgctggc
    46741 gatgcagcgt gccgtcgctg ggctgcatat tgcgccggaa tatgtgttga ttgatggtaa
    46801 ccgctgcccg aaattaccga tgcctgcgat ggctgtggtg aaaggggata gccgcgtacc
    46861 agaaatcagt gccgcgtcta tcctagcgaa agtgacgcgt gacgccgaaa tggcggcgct
    46921 ggatattgtt ttcccgcaat atggttttgc ccaacataaa gggtatccaa ccgcttttca
    46981 tctggaaaaa ctggctgaat atggcgcgac tgaacatcat cgtcgcagct ttgggcctgt
    47041 caaacgcgca ctgggacttg cgtcctgatt cttgtgtcga gattaagtaa accggaatct
    47101 gaagatgtct gaaccacgtt tcgtacacct gcgggtgcac agcgactact cgatgatcga
    47161 tggcttggcc aaaaccgcac cgctggtaaa aaaggcggcg gcgttgggta tgccagcact
    47221 ggcgatcacc gatttcacca acctttgcgg tctggtgaag ttctacggag cgggacatgg
    47281 cgcagggatt aagcctatcg tcggggcaga ttttaatgtc cagtgcgacc tgctgggtga
    47341 tgagttaacc cacctgacgg tactggcggc gaacaatacc ggctatcaga atctggcgtt
    47401 gctgatctca aaagcgtatc agcgcgggta cggtgccgcc gggccgatca tcgatcgcga
    47461 ctggcttatc gaattaaacg aagggttgat ccttctttcc ggcgggcgca tgggcgacgt
    47521 cggacgcagt cttttgcgtg gtaacagcgc gctggtagat gagtgtgtcg cgttttatga
    47581 agaacacttc ccggatcgct attttctcga gctgatccgc accggcaggc cggatgaaga
    47641 aagctatctg cacgcggcgg tggaactggc ggaagcgcgc ggtttgcccg tcgtggcgac
    47701 taacgatgtg cgctttatcg acagcagcga ctttgacgca cacgaaatcc gcgtcgcgat
    47761 ccacgacggc tttaccctcg acgatcctaa acgcccgcgt aactattcgc cgcagcaata
    47821 tatgcgtagc gaagaggaga tgtgtgagct gtttgccgac atcccagaag cccttgccaa
    47881 caccgttgag atcgccaaac gctgtaacgt aactgtgcgt cttggtgaat acttcctgcc
    47941 gcagttcccg accggggaca tgagcaccga agattatctg gtcaagcgtg caaaagaagg
    48001 cctggaagag cgtctggcat ttttattccc tgacgaggaa gaacgtctta aacgccgccc
    48061 ggaatatgac gaacgtctgg agactgaact tcaggttatc aaccagatgg gtttcccggg
    48121 ctacttcctc atcgttatgg aatttatcca gtggtcgaaa gataacggcg taccggtagg
    48181 accaggccgt ggctccggtg cgggttcact ggtggcctac gcgctgaaaa tcaccgacct
    48241 cgatccgctg gaatttgacc tgctgttcga acgtttcctt aacccggaac gtgtctccat
    48301 gcctgacttc gacgttgact tctgtatgga gaaacgcgat caggttatcg aacacgtggc
    48361 ggacatgtac ggtcgtgatg cggtatcgca gattatcacc ttcggtacga tggcggcgaa
    48421 agcggtgatc cgcgacgtag gccgcgtgct ggggcatccg tacggctttg tcgaccgtat
    48481 ctcgaaactg atcccgcccg atccggggat gacgctggcg aaagcgtttg aagccgagcc
    48541 gcagctgccg gaaatctacg aagcggatga agaagttaag gcgctgatcg acatggcgcg
    48601 caaactggaa ggggtcaccc gtaacgccgg taagcacgcc ggtggggtgg ttatcgcgcc
    48661 gaccaaaatt accgattttg cgccgcttta ttgcgatgaa gagggcaaac atccggtcac
    48721 ccagtttgat aaaagcgacg ttgaatacgc cggactggtg aagttcgact tccttggttt
    48781 gcgtacgctc accatcatca actgggcgct ggagatgatc aacaagcggc gggcgaagaa
    48841 tggcgagcca ccgctggata tcgccgcgat cccgctggat gacaagaaaa gcttcgacat
    48901 gctgcaacgc tcggaaacca cggcggtatt ccagcttgaa tcgcgcggca tgaaggacct
    48961 gatcaagcgt ctgcaacctg actgcttcga agatatgatc gcactggtgg cactgttccg
    49021 ccctggtccg ttgcagtcag ggatggtgga taactttatc gaccgtaaac atggtcgtga
    49081 agagatctcc tatccggacg tgcagtggca gcatgaaagc ctgaaatcgg tactggagcc
    49141 aacctacggc attatcttgt atcaggaaca ggtcatgcag attgcccagg tgctttctgg
    49201 ttataccctc ggtggcgcgg atatgctgcg ccgtgcgatg ggtaagaaaa agccggaaga
    49261 gatggctaag cagcgttctg tatttgctga aggtgcagaa aagaacggaa tcaacgccga
    49321 actggcgatg aaaatcttcg acctggtgga gaaattcgcg ggttacggat ttaacaaatc
    49381 gcactctgcg gcctatgctt tggtgtcata tcaaacgtta tggctgaaag cgcactatcc
    49441 ggcggagttt atggcggcgg taatgaccgc cgatatggac aacaccgaga aggtggtggg
    49501 cctggtggat gagtgctggc ggatggggct gaaaatcctg ccaccagata taaactccgg
    49561 tctttaccat ttccacgtca acgacgacgg cgaaatcgtg tatggtattg gcgccatcaa
    49621 aggggtaggt gaaggtccga ttgaggccat catcgaagcc cgtaataaag gcggctactt
    49681 ccgcgaactg tttgatctct gcgcccgtac cgacaccaaa aagttaaacc ggcgggtgct
    49741 ggaaaaactg atcatgtccg gggcgtttga ccgtcttggg ccacaccgcg cggcgctgat
    49801 gaactcgctg ggtgatgcgt taaaagcggc agatcaacac gcgaaagcgg aagctatcgg
    49861 tcaggccgat atgttcggcg tgctggccga agagccggaa caaattgaac aatcctacgc
    49921 cagctgccaa ccgtggccgg agcaggtggt attagatggg gaacgtgaaa cgttaggcct
    49981 gtacctgacc ggacacccta tcaaccagta tttaaaagag attgagcgtt atgtcggagg
    50041 cgtaaggctg aaagacatgc acccgacaga acgtggtaaa gtcatcacag ctgcggggct
    50101 cgttgttgcc gcgcgggtta tggtcaccaa gcgcggcaat cgtatcggta tctgcacgct
    50161 ggatgaccgt tccgggcggc tggaagtgat gttgtttact gacgccctgg ataaatacca
    50221 gcaattgctg gaaaaagacc gcatacttat cgtcagcgga caggtcagct ttgatgactt
    50281 cagcggtggg cttaaaatga ccgctcgcga agtgatggat attgacgaag cccgggaaaa
    50341 atatgctcgc gggcttgcta tctcgctgac ggacaggcaa attgatgacc agcttttaaa
    50401 ccgactccgt cagtctctgg aaccccaccg ctctgggaca attccagtac atctctacta
    50461 tcagagggcg gatgcacgcg cgcggttgcg ttttggcgcg acgtggcgtg tctctccgag
    50521 cgatcgttta ttaaacgatc tccgtggcct cattggttcg gagcaggtgg aactggagtt
    50581 tgactaatac aggaatacta tgagtctgaa tttccttgat tttgaacagc cgattgcaga
    50641 gctggaagcg aaaatcgatt ctctgactgc ggttagccgt caggatgaga aactggatat
    50701 taacatcgat gaagaagtgc atcgtctgcg tgaaaaaagc gtagaactga cgcgtaaaat
    50761 cttcgccgat ctcggtgcat ggcagattgc gcaactggca cgccatccgc agcgtcctta
    50821 taccctagat tacgttcgcc tggcatttga tgaatttgac gaactggctg gcgaccgtgc
    50881 gtatgcagac gataaagcta tcgtcggtgg tatcgcccgt ctcgatggtc gtccggtgat
    50941 gatcattggt catcaaaaag gtcgtgaaac caaagaaaaa attcgccgta actttggtat
    51001 gccagcgcca gaaggttacc gcaaagcact gcgtctgatg caaatggctg aacgctttaa
    51061 gatgcctatc atcaccttta tcgacacccc gggggcttat cctggcgtgg gcgcagaaga
    51121 gcgtggtcag tctgaagcca ttgcacgcaa cctgcgtgaa atgtctcgcc ttggcgtacc
    51181 ggtagtttgt acggttatcg gtgaaggtgg ttctggcggt gcgctggcga ttggcgtggg
    51241 cgataaagtg aatatgctgc aatacagcac ctattccgtt atctcgccgg aaggttgtgc
    51301 gtccattctg tggaagagcg ccgacaaagc gccgctggcg gctgaagcga tgggtatcat
    51361 cgctccgcgt ctgaaagagc tgaaactgat cgactccatc atcccggaac cgctgggcgg
    51421 cgctcaccgt aacccggaag cgatggcggc atcgttgaaa gcgcaactgc tggcggatct
    51481 ggccgatctc gatgtgttaa gcactgaaga tttaaaaaat cgtcgttatc agcgcctgat
    51541 gagctacggt tacgcgtaat tcgcaaaagt tctgaaaaag ggtcacttcg gtggcccttt
    51601 tttatcgcca cggtttgagc aggctatgat taaggaagga ttttccagga ggaacacatg
    51661 aacatcattg ccattatggg accgcatggc gtcttttata aagatgagcc catcaaagaa
    51721 ctggagtcgg cgctggtggc gcaaggcttt cagattatct ggccacaaaa cagcgttgat
    51781 ttgctgaaat ttatcgaaca taaccctcga atttgcggcg tgatttttga ctgggatgag
    51841 tacagtctcg atttatgcag cgatatcaat caacttaatg aatatctccc gctttatgcc
    51901 ttcatcaaca cccactcgac gatggatgtc agcgtgcagg atatgcggat ggcgctctgg
    51961 ttttttgaat atgcgctggg gcaggcggaa gatatcgcca ttcgtatgcg tcagtacacc
    52021 aacgaatatc ttgataacat tacgccgccg ttcacgaaag ccttgtttac ctacgtcaaa
    52081 gagcggaagt acaccttttg tacgccgggg catatgggcg gcaccgcata tcaaaaaagc
    52141 ccggttggct gtctgtttta tgattttttc ggcgggaata cccttaaggc tgatgtctct
    52201 atttcggtca ccgagcttgg ttcgttgctt gaccacaccg ggccacacct ggaagcggaa
    52261 gagtacatcg cgcggacttt tggcgcggaa cagagttata tcgttaccaa cggaacatcg
    52321 acgtcgaaca aaattgtggg tatgtacgcc gcgccatccg gcagtacgct gttgatcgac
    52381 cgcaactgcc ataaatcact ggcgcatctg ttgatgatga acgatgtagt gccagtctgg
    52441 ctgaaaccga cgcgtaatgc gttggggatt ctggggggga tcccgcgccg tgaatttact
    52501 cgcgacagca tcgaagagaa agtcgctgcc actacgcaag cacaatggcc ggttcatgcg
    52561 gtgatcacca actccaccta tgatggtttg ctctacaaca ccgactggat caaacagacg
    52621 ctggatgtcc cgtcgatcca cttcgattcc gcctgggtgc cgtatactca ttttcatcca
    52681 atctaccagg gtaaaagtgg tatgagcggc gagcgtgttg cgggaaaagt tttcttcgaa
    52741 acgcaatcga cccacaaaat gctggcggcg ttatcgcagg cttcactgat ccacattaaa
    52801 ggcgagtatg acgaagaggc gtttaacgaa gcctttatga tgcataccac cacctcgccc
    52861 agttatccca ttgttgcttc ggttgagacg gcggcggcga tgctgcgtgg taatccgggc
    52921 aaacggctga ttaaccgttc ggtggagcgg gcgctgcatt ttcgcaaaga ggtccagcgg
    52981 ctgcgggaag agtctgacgg ttggtttttc gatatctggc aaccgccgca ggtggatgaa
    53041 gccgaatgct ggcccgttgc gcctggcgaa caatggcacg gctttaacga tgcggatgcc
    53101 gatcatatgt ttctcgatcc ggttaaagtc actattttga caccggggat ggacgaacag
    53161 ggcaatatga gtgaggaggg gatcccggcg gcgctggtag caaaattcct cgacgaacgt
    53221 gggatcgtag tagagaaaac cggtccttat aacctgctgt ttctctttag tattggcatc
    53281 gataaaacca aagcaatggg attattgcgt gggttgacgg aatttaagcg ctcttacgat
    53341 ctcaacctgc ggatcaaaaa tatgctgccc gatctctatg ctgaagatcc cgatttctac
    53401 cgcaatatgc gtattcagga tctggcgcaa gggatccata agctgattcg taaacacgat
    53461 ctttccggtt tgatgttgcg ggcattcgat actttgccgg aaatgatcat gacgccacat
    53521 caggcatggc agagacagat taaaggtgaa gtagaaacca ttgcgctgga acaactggtc
    53581 ggtagagtat cggcaaatat gatcctgcct tatccaccgg gcgtaccgct gttgatgcca
    53641 ggagaaatgc tgaccaaaga gagccgcacg gtactcgatt ttctactgat gctttgttcc
    53701 gtagggcaac attaccccgg ttttgaaacg gatattcacg gcgcgaaaca ggacgaagac
    53761 ggcgtttacc gcgtacgagt cctaaaaatg gcgggataac ttgccagagc ggcttccggg
    53821 cgagtaacgt gctgttaaca aataaaggag acgttatgct gggtttaaaa caggttcacc
    53881 atattgcgat tattgcgacg gattatgcgg tgagcaaagc tttctactgc gatattcttg
    53941 gtttcacgct gcaaagcgaa gtctatcgcg aagcgcgcga ctcgtggaaa ggggatttgg
    54001 cgcttaatgg gcaatatgtg attgagcttt tctcttttcc gttcccgccg gagcgcccca
    54061 gccgaccgga agcttgcggc ctgcgtcatc tggcttttag cgttgatgac atcgatgcgg
    54121 cagtggcgca ccttgaaagc cataacgtga agtgtgaagc catccgtgtc gatccataca
    54181 cgcaaaaacg cttcaccttc tttaacgatc cggacgggct gccgttggaa ctgtatgagc
    54241 agtaaggctt gtcatcgccg catttgcccg gtaacgtgcc gggcattgct actgtaaaat
    54301 cgcaccatca tgacactcac gctcaataga ccacttctca cctcacgcca gattctggtg
    54361 gcctttagcg gcgggcttga ctccaccgtt ctgctgcatc agttggtgca gtggcggacg
    54421 gaaaatccgg gcgtcactct gcgcgctatt catgtgcatc acggtttaag tgccaatgcc
    54481 gatgcctggg ttacgcattg cgaaaacgtc tgccaacagt ggcaggtgcc gctggtggtc
    54541 gaacgcgtac aacttgcgca agaggggctg gggattgagg cccaggcgcg gcaggcgcgt
    54601 tatcaggcat ttgcccgtac cctattgccc ggcgaagtgt tggtcaccgc gcaacatctc
    54661 gacgatcaat gtgaaacctt tctgctggcg ctaaaacgcg gtagcggccc tgccgggctt
    54721 tcggctatgg cggaagtttc ggagtttgct ggaacacggc ttatccgccc gttgcttgcc
    54781 cgcacgcggg gggaactgga gcagtgggca ttggcgcatg gtttacgctg gattgaagac
    54841 gaaagtaatc aggacgactc atacgatcgt aactttctgc gcctgcgcgt agtgccgtta
    54901 ttgcagcagc gttggccgca ttttgcggaa gcaacggccc gcagcgccac actttgtgct
    54961 gaacaagaga gtctgttgga tgaactgctg gcggatgatt tagcacactg ccaaacgtcg
    55021 caggggacgc tgcagattgc gccaatgctg gcgatgagtg atgcccgtcg cgcggcgatt
    55081 attcgccgct ggctggcagg gcagaatgca ccgatgcctt cccgcgacgc gctggtgcgc
    55141 atctggcagg aagtggcgct ggcgcgggaa gatgcttccc cctgtttacg tttgggcgcc
    55201 tttgaaatcc gccgctatca gtcgcaactg tggtggatta aatccgtcac cgggcaaagc
    55261 gaaaccattg tgctgtggca gacgtggcta caaccgctgg aattaccggc ggggctggga
    55321 accgtacagc ttactgcggg aggcgatatt cgccctccgc gtgcagacga agcggtcagc
    55381 gtgcgtttca aagcgccagg attgctgcat attgtcgggc gcaacggcgg acgtaagctg
    55441 aagaaaatct ggcaagagtt aggcgtgccg ccgtggctac gtgacaccac gccactgctg
    55501 ttttatggag aaacgctgat tgcggcggca ggggtatttg tgacgcaaga aggtgtggct
    55561 gaaggtgaga atggcgtaag ttttgtctgg cagaaaacgc ttagttaagt gaaagccgga
    55621 taagacgcat caaacgtcgc atccggcgaa agtcaatcag gattcgctta ccaccaccgt
    55681 accgatttca gggtggctaa agctggtaat tttatccaga cgcagctcgc gggtttcgcc
    55741 agcggcctcg acgaccagat actccacatt tttgcgggag actaaatcac tggcttttgc
    55801 ctgcaatttt tcgccatctt tcagctcaag tgtcagcatt aaatgatgct ggcaggcgag
    55861 ctcaagatta tcgtaatcat cacaattaat tggttgatac gtatcattca ttgacataat
    55921 cgctcaccag taagtttgcc gcagcgtatg ctgctttttc cctgacagcc tcagaaaggg
    55981 cgtcgtcggc agccatttca ttcagcactt tcaaaacgca gcccagcgcg tccggaacgt
    56041 atcctaagtc tccgctggcg atttccgcgt accgcttgcg tattaactca caatattttt
    56101 ccacatgccc tcctgtcagc actctgactt aaccgtggat gcaagtctaa gcctacgaag
    56161 ataaactctg tttcgcaagg tgactatacc acactcattt ctgcaatatc agcgccgcaa
    56221 ctgcacgtat tccgttacaa tggcctcctg attcgaaagg agttttctta tggcgcttaa
    56281 agcgacaatt tataaagcga cggttaatgt ggccgatctc gaccgcaacc agtttctcga
    56341 tgcctctctg acgctggcgc gccacccttc agaaacccag gagcgtatga tgctgcgctt
    56401 gctggcgtgg ctgaaatatg ctgatgaacg tctgcaattt acccgtggtt tgtgtgccga
    56461 tgatgagccg gaagcgtggc tgcgtaacga tcacctgggc attgatttgt ggattgagct
    56521 ggggctgccg gatgagcgac gaattaagaa agcctgcacc caggccgcag aagtggcgct
    56581 gtttacctat aatagtcggg cggcgcaaat ctggtggcag caaaatcaga gcaaatgtgt
    56641 gcagtttgcc aatctttccg tctggtatct ggacgatgaa caactggcga aagtaagcgc
    56701 ctttgccgat cgtaccatga cgctgcaggc aacgattcag gatggtgtta tctggttatc
    56761 ggatgataag aataatctgg aagttaactt aaccgcctgg caacaacctt catgattgtg
    56821 atttcccgac atgttgctat tcccgatggt gagcttgaga tcaccgccat tcgtgcgcag
    56881 ggcgctggcg ggcagcatgt taataagacc tcaacggcta ttcatctgcg ttttgacatt
    56941 cgggcgccca gcctgccaga gtattacaaa gagcgtctgc tcgccgccag ccatcatttg
    57001 atcagcagtg atggcgtgat tgtcattaag gcacaggaat accgcagtca ggaactgaac
    57061 cgcgaagccg ctctggcccg gctggtggca gtgattaaag atttaacaaa agaacaaaaa
    57121 gcccgacgac ccacgcggcc cacccgtgca tcgaaagagc gcaggctggc atcgaaagca
    57181 caaaaatcaa gcgtgaaggc gatgcgcggc aaagtacgca gcggtcggga ataaaaagaa
    57241 ggaatggatg gtgaaaaaag cgatagtgac agcgatggct gtaatcagcc tctttactct
    57301 gatgggatgt aataatcggg ccgaagtcga tacgctttct ccggcgcagg ctgccgaact
    57361 gaaaccgatg ccgcaaagtt ggcgcggcgt gctaccgtgt gccgattgcg aaggaatcga
    57421 aatctctctg ttcctcgaaa aagacggaac atgggtgatg aatgagcgtt atctcggtgc
    57481 tcgtgaagaa ccttcctcct tcgcttccta cggtacatgg gcgcgaaccg ctgacaagct
    57541 ggtattaacc gacagcaaag gtgaaaagtc atattatcgg gcgaaaggcg atgcgctgga
    57601 gatgctcgat cgtgaaggca atccaattga atcgcagttc aactatacgc tggaaccggc
    57661 acaatccagt ttacctatga cgccgatgac cctgcgaggc atgtattttt atatggctga
    57721 tgcggcgacc ttcactgatt gcgcgaccgg aaaacgtttc atggtagcga ataacgcaga
    57781 gctggagcgt agctacctgg ctgcgcgcgg tcacagtgaa aaaccggtgt tactgtcagt
    57841 agaaggtcac tttacgcttg aggctaatcc ggatactggt gcgccaacca aagtattagc
    57901 gcccgatacg gcgggtaaat tttacccgaa taaggattgc agtagtttgg ggcagtaaac
    57961 ctgcttaagg cagaaacaaa cgcaaaattg cctgatgcgc tgcgcttatc aggcctacag
    58021 catataatgc aacattttga attagcacga ttttgtaggc cggataaggc gtttacgccg
    58081 catccggcat tttgtgcacg aggctggctt tatcactgag tcagcccaac aaagcgaccc
    58141 gccttgatat aaatcccagg cttcaggtgc ccaacatact gtaactgcgt ttctggctta
    58201 aacgcagaga catcaccaat acgcatatgc atcagatcgg cagggctaat ccagcccgat
    58261 tgcgccagtg taagtggatg cccggctttg gcaaatgcat cagtgacaaa ttccgaacaa
    58321 aaccacgact ttttgtctcc ttcgcacaca ctgcttaact gcgctttcgc caggtcgctg
    58381 acgcactgtt ggcgaaaatc ctcggagaac ggattcagtg agcacatctg gcgagtcacc
    58441 ataaagggaa taaattcgac aatgctgcga tagttataac cgctatcttt gattttattg
    58501 gcaaacgcgg tgatatctgt ggcttgttgc ggggtaagat ccgggactcg taagacgaaa
    58561 agcttatcac tatgcttcat cgctttttta agggaaacaa tctggacgcc agcgcctgtc
    58621 gcttctgcaa cgttattatc acccagaaag attgcaacgt gactcacaga ggaagtgctg
    58681 aagacgcgga ttccaaatga ggttacccca aggcttgagg agaacagcaa atcgccgggt
    58741 ttgagatcag gtgccgttat ttcttttatt gattgttcgg tgaaagagct ttgatgctgg
    58801 aatttgacag cccatgtttt tgcctcggca tctactgctg tggctgatgg gtctggctgg
    58861 ctgatatcaa cggtacaagc cgaaagtaaa agaaagctgg ggagaagcag gcggcagtac
    58921 gcctttggtt tatccatttt atacaatcca tgtaaaaaag ggccctgaaa ttcaggaccc
    58981 tttctggcat cagcctttaa tctgtttcac cagatattcg acgatgtcac cagtcttaat
    59041 taactgtttc tcgccgttgc gacgatattt atattcgata tcgtcgttgt cgaggttacg
    59101 gtcgcccagc acaatagtgt gcggaatacc gatcagttcc atatcagcaa acatcacgcc
    59161 cggacgctct ttgcggtcat ccagcagcac ttcgatacct tgtgcacgca gttcgctgta
    59221 cagtttctca gcaagctctt gtacgcggaa ggatttgtgc atgttcatcg gcagaatcgc
    59281 cacctggaac ggcgcgatag cgtcaggcca tacgatgccg cgttcgtcgt agttctgctc
    59341 aatcgccgca gctaccacac gcgttacccc gataccgtag caacccatcg tcaggatttg
    59401 gttacggcca tcttcaccct gtacggaggc tttcagtgct tcggagtact tggtacccag
    59461 ctggaagatg tgaccaactt cgataccacg tttgatcagc agcgtaccct ggccatccgg
    59521 gcttggatcg ccagccacca cgttacggat atctgcaact tccggggtag cgacatcgcg
    59581 atcccagttg ataccgaagt agtgtttacc atcgatgtta gcaccagcag cgaaatcact
    59641 catcgccgca acggtacggt caatcaccac cggaatcggc atgtttaccg gacccagtga
    59701 acccggaccg gctttaacca cagcacgaat ttcttcttcg gtcgcgaaag tcagcgggct
    59761 tgcaacctgc ggcagttttt ctgctttaac ttcgttcagc tcgtgatcac cgcgcaccag
    59821 cagcgcaacc agcgggaagc tactgccttc aaccgcttta accagcagag tcttaaccgt
    59881 tttctcaatc ggcagattga actgttcaac cagttccgcg atggttttcg cgttcggcgt
    59941 atcaaccagc gtcatttcct gggtagcagc agcgcgcggt tctttcggcg cgatagcttc
    60001 tgccagttca atgttcgctg catagtcaga ggtgtcggag aagaccacat cgtcttcacc
    60061 gctctgcgcc agcacctgga attcgtgaga ggcgctgccg ccgatagaac cggtgtcggc
    60121 ctgtacggcg cggaaatcca gccccatgcg gctgaagatt ttgctgtagg ccgcatacat
    60181 tgcatcgtag gtttcctgca gggattcctg agaagtatgg aaagagtaag catctttcat
    60241 caggaattcg cgggaacgca tgacgccgaa acgcggacgc acttcgtcgc ggaacttggt
    60301 ctggatctga tagaagttca gcggcagctg tttgtaagag ctaagctcgt tacgaatcag
    60361 gtcagtgata acttcttcat gagttgggcc gagtacgaac ggacgctcgc cacggtcaac
    60421 aaaacgcagc agttccggac cgtactgttc ccaacgacca ctctcttgcc acaaatcggc
    60481 tggctgaacc accggcatcg acacctcgat cgcaccggcg ttgttcatct cttcacgcac
    60541 gatgttttcg acttttttca gaacgcgcac gccggtcggc agccaggtat ataacccgga
    60601 ggccagcttg cggatcatcc cggcgcgcag catcagctga tggctgatca cctcggcgtc
    60661 ggcaggtgtc tccttgagag tggagagcag gtattggcta gtacgcatgt tgttacggtt
    60721 ccagttggaa ggtagaacag gctcaaggcg agcctgggac aaaaaaagtg atttagttta
    60781 ccagtgcaaa agaaatgtca aaagagaagg gcgtgaattt aacgcggttc cagcgcaaag
    60841 acttcaaaac ctgcgtcggt gacgcgccag cgaacgttaa aatcatgtag ccagacggca
    60901 taggttttgc ccgtttcctc acctttacga taggccgggc gcgggtcctg cgccagtact
    60961 tcgcggataa acagcgttaa ctgcggataa cgcttctcca gcgtcaaaag ctgcttttcg
    61021 acctctgcgg taaaactcac cgccatctct gcagctggcg cactttgcgc atagctggca
    61081 ctggcatcgg gaagcgattc ggcaaagggg agatacggtt tgatatccac taccggcgta
    61141 ccatcgacca gatccagact gccgagcttc agaatcacgc tgtctttatg gcaaacaacc
    61201 tctttcagct ctaccagcga catgccaatt gggttagggc ggaaagtaga gcgtgttgcg
    61261 aaaaccccca ttctggcgtt accgccgagg cgcggtggac gcacagtcgg acgccagccg
    61321 ccttccatcg tttgatgaaa gacgaaaagg atccataaat ggctgaatgc ttccaggccg
    61381 cgaacggcgt cggcctggtt gtaggaagca atgagatgca gttcgccgtt ggcgcttttt
    61441 accagacctg gctggcgcgg aacggcgaat ttttctttat agggcgagcg aataacgcct
    61501 atttgctcga actggaaact gctcatttcg ccgtaatgtt aagcgcagag ccgatacata
    61561 cagcctgacg atagcagccc ggcgtaccgc tggtgacttc gcagctatgc agtaatacag
    61621 cattggcttt cattttagag gcattgattt gcatctgctt acgtgcggtt ggaatgctcg
    61681 gcggagagtc ctgattagag gcctggcaag agtcgccact gacttcaccg agatcgcgga
    61741 acggtttgcc gactaattct tctgcattgg tataaattcg gaccggcgtg gcgcgcggcg
    61801 ctttcggttt tgcaggctcc gctttcggct ggggtgcagt gctttgaacg ggttcgacag
    61861 gggatctgct taacatggaa cagccgctta gcatgagtgc tactaaacag atcggtaaag
    61921 cacgcatagt atttcctcaa tgtatgatca aaacgtcaat attgaatcag gagcttgtaa
    61981 aaatgacaag acgggcaagc gcccgtcctg aatgatatta caaattgtgg aaacagccta
    62041 aaaattacca gcctttaaca gcgccgccgt taaacacttt gtttgctgct tcgtaaactt
    62101 catcagactg ataagcctgg acgaatttct tcacgttctc ggcgtctttg ttatcttcac
    62161 gcgttacgat caggtttacg tatggggagt ctttatcttc gacaaagata ccgtctttcg
    62221 ccggagtcag gccaatctgg ctggcatagg tggtattgat aactgccaga gcgatttgcg
    62281 cgtcgtccag agaacgcggc agttgcggtg cttccagttc aacaattttc agattttttg
    62341 ggttctcaac aacatcaaga actgtcggca gcaggccaac gccatctttc agtttgatca
    62401 agcccacttt ttgcagcagc agcagtgaac gaccaagatt agttggatcg tttggcacgg
    62461 caacctgcga accatcctgc agttcatcca gtgatttgat tttcttggag taaccagcaa
    62521 tcggataaac aaaagtgttg cctactgcga ccagtttgta gccacgatct ttcagttgct
    62581 gatcaaggta cggtttatgc tggaaggcgt tggcgtcgat atcgcctttg ctcaatgctt
    62641 cgtttggcag aacatagtcg ttgaaggtta ccagctcaac gtccaggcca tatttgtctt
    62701 tcgcaacttt ctgcgcgact tctgctacct gctgttcggc accaacaatc acgccgactt
    62761 taatgtggtt tggatctttt tcatcctgac cgcagcctac cagtgccagt gatccaatca
    62821 gggctcccac tgccgcaaag gttttgaatt tgaacgccat accttattcc ttcttcaatt
    62881 atttatgttg tgttgaacgt tacttgcgag tgacagcccg gacgatgcgg tcgcctgcga
    62941 actgaattaa ataaaccaga atgaccagca ataccagtac cgtattcatc accgtcgcgt
    63001 tgtagccgat gtagccatac tgatagccaa tctgacctaa accaccggca ccgactgcac
    63061 cacccatcgc ggaataaccg accagggtaa tcagggtgat agttgccgca ttcaccagac
    63121 ccggcaacgc ttccggtaac agcactttac ggacgatctg cattggcgtg gcacccattg
    63181 cgcgggaagc ttcaattaac ccggttggga tctccagcag agcgttctcg accatacggg
    63241 caataaacgg tgctgcacca acggttaacg gaacaattgc tgcctgcaaa ccaatcgatg
    63301 taccgacaat aacgcgggta aacggaatca tccataccag caagataatg aacgggatgg
    63361 aacggaaaat gttcacaatc gcagaaacgg tacgatacag cttcgcgtta gcaataattt
    63421 gccccggacg cgtgacataa agcagaacgc caaccggcag accaatcaca aacccaaaaa
    63481 atccggatac gaaggtcatt gccagcgttt cccatacgcc acgaaccagc agccacatca
    63541 tcggctcaga cataacccag tacctctact tttacatggt gttcctgcag ccaggcaatg
    63601 gcggcttgcg tatcttgttg tgtgccgtgc atttcagtca gcatgatgcc gaatttaacg
    63661 ccaccggcgt agtccatctg cgcgctaata atgttgttgt tgacgttgaa acgacgcgcg
    63721 gtttcagaaa gcagtggggc atcgaccgat tgaccggtaa actccagacg cagcatcggc
    63781 acgcagtcag taaatggctc cgtttgcaga cgttcctggt aatcttccgg gatatccaga
    63841 tgcagggtcg actgaataaa cttctgcgcc agcggcgttt tcggatgcga gaacacttca
    63901 cttaccgtgt cctgctcgat cagttcgcca ttgctgatga ccgccacgca atcacaaata
    63961 cgcttcacaa cgtccatttc gtgggtgatc aacagaatcg tcaaacccag acggcggtta
    64021 atgtctttca gcagttcgag aatagaacgt gtcgttgccg ggtccagcgc gctggtggct
    64081 tcatcacaca gcaatacttt gggattgctg gctaatgcac gggcaatcgc cacacgttgt
    64141 ttctgcccac cggaaagatt cgacgggtag ctatcatgct tatcgccaag accaaccaat
    64201 gacagcaatt ccgtcacgcg acgtttgatc tcgtctttcg gtgtgttgtc cagctccagc
    64261 ggcagagcca cgttgccaaa aacagtacgc gaagagagca ggttaaaatg ctggaaaatc
    64321 ataccaatct ggcggcgagc tttggtcaac tcggattctg acagcgtggt caattcctgg
    64381 ccatcgacca gcacgctacc ctcggttggg cgctccagca ggtttacaca acgtataagc
    64441 gtactcttac ccgcgcctga ggcaccgata acgccataaa tttgtccagc tggcacatgc
    64501 aggctgacgt tgttcaacgc ctggatggtg cgggtgccct ggtggaacac tttggtgata
    64561 ttcgaaagtt ttatcattga ttatttatta tcgtcattaa gttagtcgta gcatctcgaa
    64621 tgcctgaaac gggcaacgcc gtcaatgaaa tggatgttaa ggcatccaga cgtctaaatc
    64681 aatcaggttt atgcgaagag cactttcttg caggtcgaaa catgcgatac tagcgtccca
    64741 tgccttatta aggagctata aaaggtggcg aagagcgtac ccgcaatttt tcttgaccgt
    64801 gatggcacca ttaatgtcga tcacggctat gtccatgaga tcgacaactt tgaatttatc
    64861 gacggtgtta ttgacgccat gcgcgagcta aaaaaaatgg gctttgcgct ggtggtagta
    64921 accaaccagt ctggcattgc tcgcggtaaa tttaccgaag cacagtttga aacgctgacc
    64981 gagtggatgg actggtcgct ggcggaccga gatgtcgatc tggatggtat ctattattgc
    65041 ccgcatcatc cgcaggggag tgttgaagag tttcgccagg tctgcgattg ccgcaaacca
    65101 catccgggga tgcttttgtc agcacgcgat tatttgcata ttgatatggc cgcttcttat
    65161 atggtgggcg ataaattaga agatatgcag gcagcggttg cggcgaacgt gggaacaaaa
    65221 gtgctggtgc gtacgggtaa acctattaca cctgaagcag aaaacgcggc agattgggtg
    65281 ttaaatagcc tggcagacct gccgcaagcg ataaaaaagc agcaaaaacc ggcgcaatga
    65341 ttaaaagatg aacggttgaa ataaaaatgc atttttccgc ttgtcttcct gagccgactc
    65401 cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc
//
LOCUS       DAJTYG010000009        65347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTYG010000009 DAJTYG010000000
VERSION     DAJTYG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.009"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            144..473
                     /locus_tag="OO568_000633"
     CDS             144..473
                     /locus_tag="OO568_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6640116.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            721..966
                     /gene="nrdH"
                     /locus_tag="OO568_000634"
     CDS             721..966
                     /gene="nrdH"
                     /locus_tag="OO568_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6640117.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            963..1373
                     /gene="nrdI"
                     /locus_tag="OO568_000635"
     CDS             963..1373
                     /gene="nrdI"
                     /locus_tag="OO568_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR6640118.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            1346..3490
                     /gene="nrdE"
                     /locus_tag="OO568_000636"
     CDS             1346..3490
                     /gene="nrdE"
                     /locus_tag="OO568_000636"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6640119.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3500..4459
                     /gene="nrdF"
                     /locus_tag="OO568_000637"
     CDS             3500..4459
                     /gene="nrdF"
                     /locus_tag="OO568_000637"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6640120.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            4814..6016
                     /gene="proV"
                     /locus_tag="OO568_000638"
     CDS             4814..6016
                     /gene="proV"
                     /locus_tag="OO568_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6640121.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6009..7073
                     /gene="proW"
                     /locus_tag="OO568_000639"
     CDS             6009..7073
                     /gene="proW"
                     /locus_tag="OO568_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6640122.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGACSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            7131..8123
                     /gene="proX"
                     /locus_tag="OO568_000640"
     CDS             7131..8123
                     /gene="proX"
                     /locus_tag="OO568_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6640123.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            8315..9494
                     /locus_tag="OO568_000641"
                     /pseudo
     CDS             8315..9494
                     /locus_tag="OO568_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9618..10355
                     /gene="ygaZ"
                     /locus_tag="OO568_000642"
     CDS             9618..10355
                     /gene="ygaZ"
                     /locus_tag="OO568_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6640124.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            10345..10680
                     /gene="ygaH"
                     /locus_tag="OO568_000643"
     CDS             10345..10680
                     /gene="ygaH"
                     /locus_tag="OO568_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6640125.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            10771..11301
                     /gene="emrR"
                     /locus_tag="OO568_000644"
     CDS             10771..11301
                     /gene="emrR"
                     /locus_tag="OO568_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6640126.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPNQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            11428..12600
                     /gene="emrA"
                     /locus_tag="OO568_000645"
                     /pseudo
     CDS             11428..12600
                     /gene="emrA"
                     /locus_tag="OO568_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            12617..14155
                     /gene="emrB"
                     /locus_tag="OO568_000646"
     CDS             12617..14155
                     /gene="emrB"
                     /locus_tag="OO568_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6640127.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(14219..14734)
                     /gene="luxS"
                     /locus_tag="OO568_000647"
     CDS             complement(14219..14734)
                     /gene="luxS"
                     /locus_tag="OO568_000647"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6640128.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGSHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(14884..16440)
                     /gene="gshA"
                     /locus_tag="OO568_000648"
     CDS             complement(14884..16440)
                     /gene="gshA"
                     /locus_tag="OO568_000648"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6640129.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFHDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(16513..16941)
                     /locus_tag="OO568_000649"
     CDS             complement(16513..16941)
                     /locus_tag="OO568_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6640130.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(16938..17504)
                     /gene="yqaB"
                     /locus_tag="OO568_000650"
     CDS             complement(16938..17504)
                     /gene="yqaB"
                     /locus_tag="OO568_000650"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6640131.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(17652..17728)
                     /locus_tag="OO568_000651"
     tRNA            complement(17652..17728)
                     /locus_tag="OO568_000651"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17692..17694),aa:Arg,seq:acg)
     gene            complement(17793..17869)
                     /locus_tag="OO568_000652"
     tRNA            complement(17793..17869)
                     /locus_tag="OO568_000652"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17833..17835),aa:Arg,seq:acg)
     gene            complement(17873..17965)
                     /locus_tag="OO568_000653"
     tRNA            complement(17873..17965)
                     /locus_tag="OO568_000653"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17929..17931),aa:Ser,seq:gct)
     gene            complement(18281..18466)
                     /gene="csrA"
                     /locus_tag="OO568_000654"
     CDS             complement(18281..18466)
                     /gene="csrA"
                     /locus_tag="OO568_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6640132.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(18701..21331)
                     /gene="alaS"
                     /locus_tag="OO568_000655"
     CDS             complement(18701..21331)
                     /gene="alaS"
                     /locus_tag="OO568_000655"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6640133.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(21459..21959)
                     /gene="recX"
                     /locus_tag="OO568_000656"
     CDS             complement(21459..21959)
                     /gene="recX"
                     /locus_tag="OO568_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6640134.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(22202..23263)
                     /gene="recA"
                     /locus_tag="OO568_000657"
     CDS             complement(22202..23263)
                     /gene="recA"
                     /locus_tag="OO568_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6640135.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(23343..23840)
                     /gene="pncC"
                     /locus_tag="OO568_000658"
     CDS             complement(23343..23840)
                     /gene="pncC"
                     /locus_tag="OO568_000658"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6640136.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYVLQTLWQQ
                     FLQNT"
     gene            complement(23985..25070)
                     /gene="mltB"
                     /locus_tag="OO568_000659"
     CDS             complement(23985..25070)
                     /gene="mltB"
                     /locus_tag="OO568_000659"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6640137.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            25326..25889
                     /gene="srlA"
                     /locus_tag="OO568_000660"
                     /gene_synonym="gutA"
     CDS             25326..25889
                     /gene="srlA"
                     /locus_tag="OO568_000660"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR6640138.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            25886..26845
                     /gene="srlE"
                     /locus_tag="OO568_000661"
                     /gene_synonym="gutE"
     CDS             25886..26845
                     /gene="srlE"
                     /locus_tag="OO568_000661"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6640139.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            26856..27227
                     /gene="srlB"
                     /locus_tag="OO568_000662"
                     /gene_synonym="gutB"
     CDS             26856..27227
                     /gene="srlB"
                     /locus_tag="OO568_000662"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6640140.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSMAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            27231..28010
                     /gene="srlD"
                     /locus_tag="OO568_000663"
     CDS             27231..28010
                     /gene="srlD"
                     /locus_tag="OO568_000663"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR6640141.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            28115..28474
                     /gene="gutM"
                     /locus_tag="OO568_000664"
     CDS             28115..28474
                     /gene="gutM"
                     /locus_tag="OO568_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6640142.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGHFKPRVVVAIALDDQQCIVDILFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            28541..29314
                     /gene="srlR"
                     /locus_tag="OO568_000665"
                     /gene_synonym="gutR"
     CDS             28541..29314
                     /gene="srlR"
                     /locus_tag="OO568_000665"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6640143.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            29307..30272
                     /gene="gutQ"
                     /locus_tag="OO568_000666"
     CDS             29307..30272
                     /gene="gutQ"
                     /locus_tag="OO568_000666"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6640144.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(30269..31783)
                     /gene="norR"
                     /locus_tag="OO568_000667"
     CDS             complement(30269..31783)
                     /gene="norR"
                     /locus_tag="OO568_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6640145.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRVVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            31970..33409
                     /gene="norV"
                     /locus_tag="OO568_000668"
     CDS             31970..33409
                     /gene="norV"
                     /locus_tag="OO568_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR6640146.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHCREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            33406..34539
                     /gene="norW"
                     /locus_tag="OO568_000669"
     CDS             33406..34539
                     /gene="norW"
                     /locus_tag="OO568_000669"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6640147.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(34667..36919)
                     /gene="hypF"
                     /locus_tag="OO568_000670"
     CDS             complement(34667..36919)
                     /gene="hypF"
                     /locus_tag="OO568_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6640148.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(37072..37599)
                     /gene="hydN"
                     /locus_tag="OO568_000671"
     CDS             complement(37072..37599)
                     /gene="hydN"
                     /locus_tag="OO568_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6640149.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(37748..38758)
                     /gene="ascG"
                     /locus_tag="OO568_000672"
     CDS             complement(37748..38758)
                     /gene="ascG"
                     /locus_tag="OO568_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6640150.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            39018..40475
                     /gene="ascF"
                     /locus_tag="OO568_000673"
     CDS             39018..40475
                     /gene="ascF"
                     /locus_tag="OO568_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR6640151.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            40484..41908
                     /gene="ascB"
                     /locus_tag="OO568_000674"
     CDS             40484..41908
                     /gene="ascB"
                     /locus_tag="OO568_000674"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR6640152.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQDD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYTSRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(42023..42442)
                     /locus_tag="OO568_000675"
     CDS             complement(42023..42442)
                     /locus_tag="OO568_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6640153.1"
                     /translation="MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(42439..42750)
                     /locus_tag="OO568_000676"
     CDS             complement(42439..42750)
                     /locus_tag="OO568_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCR6640154.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(42923..43666)
                     /locus_tag="OO568_000677"
     CDS             complement(42923..43666)
                     /locus_tag="OO568_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640155.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFDKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(43718..44188)
                     /gene="hycI"
                     /locus_tag="OO568_000678"
     CDS             complement(43718..44188)
                     /gene="hycI"
                     /locus_tag="OO568_000678"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR6640156.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(44181..44591)
                     /gene="hycH"
                     /locus_tag="OO568_000679"
     CDS             complement(44181..44591)
                     /gene="hycH"
                     /locus_tag="OO568_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR6640157.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(44588..45355)
                     /gene="hycG"
                     /locus_tag="OO568_000680"
     CDS             complement(44588..45355)
                     /gene="hycG"
                     /locus_tag="OO568_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR6640158.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGILHDLYCVWGGTDKIVPVDVYIPSCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(45355..45897)
                     /gene="hycF"
                     /locus_tag="OO568_000681"
     CDS             complement(45355..45897)
                     /gene="hycF"
                     /locus_tag="OO568_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR6640159.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(45907..47591)
                     /gene="hycE"
                     /locus_tag="OO568_000682"
                     /pseudo
     CDS             complement(45907..47591)
                     /gene="hycE"
                     /locus_tag="OO568_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
     gene            complement(47609..48505)
                     /gene="hycD"
                     /locus_tag="OO568_000683"
     CDS             complement(47609..48505)
                     /gene="hycD"
                     /locus_tag="OO568_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6640160.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIGSPLPQLGDLITLLYLFAIARFFF
                     AISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPL
                     SQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLK
                     QLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDI
                     TPRITWAGFGFAFLAFVSLLAA"
     gene            complement(48508..50385)
                     /gene="hycC"
                     /locus_tag="OO568_000684"
     CDS             complement(48508..50385)
                     /gene="hycC"
                     /locus_tag="OO568_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6640161.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGSVGVSGAMSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGSDIWLLGVIGFGLLAGI
                     IPLHGWVPQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMI
                     TAFVGGLYALMEHNIQRLLAYHTLENIGIILLGLGLGLGLGLGLGAGVTGIALEQPAL
                     IALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVG
                     LMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAK
                     VYGVTFLGAPRTKEAENATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPL
                     EPANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITA
                     HGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRG
                     A"
     gene            complement(50382..50993)
                     /gene="hycB"
                     /locus_tag="OO568_000685"
     CDS             complement(50382..50993)
                     /gene="hycB"
                     /locus_tag="OO568_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6640162.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPLLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVSGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(51118..51579)
                     /gene="hycA"
                     /locus_tag="OO568_000686"
     CDS             complement(51118..51579)
                     /gene="hycA"
                     /locus_tag="OO568_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6640163.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            51791..52141
                     /gene="hypA"
                     /locus_tag="OO568_000687"
     CDS             51791..52141
                     /gene="hypA"
                     /locus_tag="OO568_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6640164.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            52145..53017
                     /gene="hypB"
                     /locus_tag="OO568_000688"
     CDS             52145..53017
                     /gene="hypB"
                     /locus_tag="OO568_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6640165.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            53008..53280
                     /gene="hypC"
                     /locus_tag="OO568_000689"
     CDS             53008..53280
                     /gene="hypC"
                     /locus_tag="OO568_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6640166.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            53280..54401
                     /gene="hypD"
                     /locus_tag="OO568_000690"
     CDS             53280..54401
                     /gene="hypD"
                     /locus_tag="OO568_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6640167.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            54398..55408
                     /gene="hypE"
                     /locus_tag="OO568_000691"
     CDS             54398..55408
                     /gene="hypE"
                     /locus_tag="OO568_000691"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6640168.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDCLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAAVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            55482..57560
                     /gene="flhA"
                     /locus_tag="OO568_000692"
     CDS             55482..57560
                     /gene="flhA"
                     /locus_tag="OO568_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR6640169.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(57597..57950)
                     /locus_tag="OO568_000693"
     CDS             complement(57597..57950)
                     /locus_tag="OO568_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6640170.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(57982..58161)
                     /locus_tag="OO568_000694"
     CDS             complement(57982..58161)
                     /locus_tag="OO568_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640171.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMFMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            58237..60798
                     /gene="mutS"
                     /locus_tag="OO568_000695"
     CDS             58237..60798
                     /gene="mutS"
                     /locus_tag="OO568_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6640172.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            60904..61556
                     /gene="pphB"
                     /locus_tag="OO568_000696"
                     /pseudo
     CDS             60904..61556
                     /gene="pphB"
                     /locus_tag="OO568_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(61607..62374)
                     /locus_tag="OO568_000697"
     CDS             complement(61607..62374)
                     /locus_tag="OO568_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6640173.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            62570..63478
                     /locus_tag="OO568_000698"
     CDS             62570..63478
                     /locus_tag="OO568_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6640174.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            63475..64737
                     /locus_tag="OO568_000699"
                     /pseudo
     CDS             63475..64737
                     /locus_tag="OO568_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            64734..>65347
                     /locus_tag="OO568_000700"
     CDS             64734..>65347
                     /locus_tag="OO568_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6640175.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAG"
BASE COUNT        15462 a        17611 c        16920 g        15354 t
ORIGIN      
        1 ctcgttattt tcaaccgcgg gcctacctta cctgattgcg cattcacaac gctttcagaa
       61 aagtccataa agccgcgatg aacctcagtt aagaaaatta tggtctacac tgaaaattac
      121 atcgaattct aatgggggat catatgttta accgaccgaa ccgcaacgac gttgatgatg
      181 gcgtgcagga tatccagaat gatgtcaatc aattagctga cagcctcgaa tctgtattga
      241 agtcctgggg cagcgacgcc aaaggggaag ctgaagccgc acgcagtaaa gctcaggcgt
      301 tgctgaaaga aaccagggcg cgaatgcatg gtcgtactcg cgtccagcaa gccgcgcgcg
      361 atgccgttgg ctgcgcggat tcttttgttc gtgaaagacc ctggtgtagc gtgggtacag
      421 cagctgcggt aggtattttt atcggcgcac tgttaagcat gcgcaaatcg tagtgcaaaa
      481 atgataataa atacgcgtct ttgaccccga agcctgtctt cggggtttct ttttgcctgg
      541 tgaatcacaa aaatccccct cccccgtcac gctcatatcc agggtaattt cgatcactat
      601 ttgctatata ttgtgtggtt gaatcttttt tcaactacat ctagtatctc tgtatcaaca
      661 gagagacaac ccgacgcgta tcatcgcgcc gtatcttcat tttaaacaga aatacgaatc
      721 atgcgcataa ctatttatac tcgtaacgat tgcgttcagt gccacgccac caaacgggcg
      781 atggaaaacc ggggctttga ttttgaaatg attaatgtcg atcgcgttcc tgaagcagcg
      841 gaagcgttgc gtgctcaggg ctttcgtcag ttgccggtag tgattgctgg cgatcttagc
      901 tggtctggtt tccgtccgga catgattaac cgtctgcatc cagcgccaca cgcggccagt
      961 gcatgagcca gctcgtctac ttctccagca gctccgaaaa cacgcagcgt tttatcgaac
     1021 gtttaggtct gcccgcggtg cgcatcccgc tcaatgagcg ggaacggatt caggtagacg
     1081 agccttacat cctgatcgtg ccctcttacg gcggcggcgg tacggctggc gcggtgccac
     1141 gacaggtaat tcgcttttta aacgacgagc acaaccgggc gttgcttcgc ggcgttattg
     1201 cttctggtaa tcgcaacttt ggtgaggcgt atggccgcgc cggagatgtg attgcccgga
     1261 aatgcggcgt gccgtggctg taccgttttg aactcatggg tacgcaaagc gatatcgaaa
     1321 acgttcgtaa aggagtaacc gaattttggc aacgacaacc gcagaatgcc tgacgcagga
     1381 aacgatggat taccacgcgc tgaatgcgat gcttaatctt tacgatagcg caggtcgcat
     1441 tcagttcgat aaagaccgcc aggccgttga cgcctttatt gcgacgcatg tgcgtccgaa
     1501 cagtgtgacc ttcagtagcc agcagcagcg cctgaactgg ctggtcaacg aaggttacta
     1561 tgatgaaagc gttcttaatc gctactctcg cgactttgtc attacgctgt ttgcccacgc
     1621 acacaccagc ggttttcgtt tccagacatt cctcggggca tggaagtttt acaccagcta
     1681 tacgttgaag acattcgacg gtaaacgtta tctggaagat tttgccgatc gagtcacgat
     1741 ggtggcgctg acgctggcac aaggcgatga gacgctggcg ttgcaactga ccgatgaaat
     1801 gctgtcagga cgctttcagc cagccacgcc aacattcctc aactgcggta agcagcagcg
     1861 cggcgaactg gtttcctgtt ttttgctgcg tattgaagac aatatggagt cgattggtcg
     1921 ggcggtaaat tccgcactgc agctgtcgaa acgcggcggc ggcgtagcat ttttgctgtc
     1981 gaatctgcgg gaagcgggcg cgccaattaa acgtattgaa aatcaatctt ctggcgtaat
     2041 tccggtgatg aaaatgttgg aagacgcatt ttcctatgcc aaccaactcg gcgcgcgtca
     2101 gggggcaggt gcagtctatt tacatgctca tcatcccgat attctgcgtt ttctcgacac
     2161 gaaacgggaa aatgccgacg aaaaaatccg cattaaaaca ctgtcgctgg gcgtggtgat
     2221 cccggatatc actttccatc tggcaaaaga gaatgcgcag atggcgctgt tttcgcccta
     2281 tgacgttgag cgagtttatg gcaagccgtt tgccgatatc gccattagcg aacactatga
     2341 cgaactggtt gccgatgaac gcatccgcaa aaaatacctc aacgcccgtg atttcttcca
     2401 gcgactggca gaaatccagt ttgagtccgg ctatccctac atcatgtatg aagacacagt
     2461 aaaccgtgct aaccctatcg ccgggcgcat aaatatgagt aatctctgct cagaaatttt
     2521 gcaggttaac agcgcctcag agtatgacga gaatctcgac tatgcccgca caggccatga
     2581 tatttcctgc aatttaggtt cgttgaatat tgcgcacacc atggattccc ccgatttttc
     2641 ccgcacggta gagactgccg tgcgcggttt aacggcggtg tcagatatga gtcatatccg
     2701 cagcgtgccg tccatcgaag ccggaaatgc cgcctcgcac gccatcggac tggggcagat
     2761 gaatttacat ggttatctgg cgagggaagg catcgcttat ggttcgccgg aagccctgga
     2821 tttcaccaat ctctatttct ataccatcac ctggcacgca ctgcgtacct cgatgttgct
     2881 ggcgcgcgaa cgcggtgaaa ccttcgccgg gttcaaacag tcacgctatg ccagtggtga
     2941 atatttcagc caatatctgc aaggtaactg gcagccgaaa acggcgaaag ttggcgaact
     3001 gtttgcccgc agcggtatta cgttacctac ccgtgagatg tgggcgcaac tgcgcgacga
     3061 cgtgatgcgc tacggcatat acaaccagaa tctccaggcg gtgccgccaa ccgggtctat
     3121 ctcttatatc aaccatgcta cgtcgagtat tcatccgatt gtggcgaaag tagagatacg
     3181 caaagagggc aaaacaggac gcgtttacta ccctgccccg tttatgacta acgaaaatct
     3241 ggcgctgtat caggacgctt acgaaattgg cgcagaaaag atcatcgaca cctacgcgga
     3301 agcgactcgc catgtcgatc aggggctgtc gctgacgctg tttttccccg ataccgccac
     3361 cactcgcgat atcaacaaag cgcagattta cgcctggcgc aaaggtatca aaacgctcta
     3421 ttacatccgc ctgcgtcaga tggcgctgga aggcactgaa attgaaggct gcgtctcctg
     3481 tgcactttaa ggaatatcta tgaaactctc acgtatcagc gccatcaact ggaacaagat
     3541 atctgacgat aaagatctgg aggtgtggaa tcgcctgacc agcaatttct ggctaccaga
     3601 aaaggtgccg ctgtcgaacg atattcccgc ctggcagaca ttaactgtcg tagaacaaca
     3661 actgacgatg cgcgttttta ctggcctgac gctgctcgac acgctgcaaa atgttatcgg
     3721 cgcgccttcg ctgatgcccg atgcgctcac gcctcatgaa gaggcggtat tatcgaatat
     3781 cagctttatg gaagcggttc atgcccgctc ttacagttcg attttctcga cgctgtgcca
     3841 gaccaaagat gtcgatgccg cctacgcctg gagtgaagaa aacgcaccgt tgcagcgaaa
     3901 agctcagatt attcagcaac attatcgcgg tgatgatccg ctgaaaaaga aaatcgccag
     3961 tgtgtttctt gaatcttttt tgttctattc cggtttctgg ctgccgatgt atttttccag
     4021 ccgcggaaag ctgaccaata ccgcggacct gatccgtctg attatccgcg atgaagcagt
     4081 ccacggttac tacataggct ataaatatca gaaaaacatg gaaaagatat ctctgggaca
     4141 acgtgaagag ttgaagagtt tcgccttcga tttgttgctg gaactctacg acaacgagtt
     4201 gcaatacacc gatgagctgt acgccgaaac cccgtgggct gacgatgtga aagcgtttct
     4261 ctgttacaac accaataagg ctttgatgaa tctgggctac gaaccgttat ttcccgcaga
     4321 aatggcggaa gtgaatccgg caatcctcgc cgcgctttcg ccgaatgccg atgaaaatca
     4381 cgatttcttt tccggttcag gctcctctta tgtgatgggg aaagcggttg aaacagaaga
     4441 tgaagactgg aatttctgag ggtgttattt tcaaaaatat cactacccgc agcagggaaa
     4501 taattcccgc caaatagctt tttatcacgc aaatattctg tagtgatcta cactgatact
     4561 ctgttgcatt attcgcctga aactacaata ttcaggcgtt ttttcgctat ctatgacaaa
     4621 aaatatcaac ttttctcgat ttgctctcag cccttatatc acggaaaatt ccggcgattt
     4681 gctcgcatca atattcatgc cacatttgcc ctcaggggtt gcctcagatt ctcagtatgt
     4741 tagggtagaa aaaagtgact attcccattg ggtaatatat cgacataaac aaataaagga
     4801 atcattctat tgcatggcca ttaaattaga aattaaaaat ctttataaaa tatttggtga
     4861 gcatccacag cgagcgttca aatatatcga acaaggactt tcaaaagaac aaattctgga
     4921 aaaaactggg ctatcgcttg gcgtaaaaga cgccagtctg gccattgaag aaggcgagat
     4981 atttgtcatc atgggattat ccggctcggg taaatccaca atggtacgcc ttctcaatcg
     5041 cctgattgaa cccacccgcg ggcaagtgct gattgatggt gtggatattg ccaaaatatc
     5101 cgacgccgaa ctccgtgagg tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc
     5161 cttaatgccg catatgaccg tgctggacaa tactgcgttc ggtatggaat tggccggaat
     5221 taatgccgaa gaacgccggg aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa
     5281 ttatgcccac agctacccgg atgaactctc tggcgggatg cgtcaacgtg tgggattagc
     5341 ccgcgcgtta gcgattaatc cggatatatt attaatggac gaagccttct cggcgctcga
     5401 tccattaatt cgcaccgaga tgcaggatga gctggtaaaa ttacaggcga aacatcagcg
     5461 caccattgtc tttatttccc acgatcttga tgaagccatg cgtattggcg accgaattgc
     5521 cattatgcaa aatggtgaag tggtacaggt cggcacaccg gatgaaattc tcaataatcc
     5581 ggcgaatgat tatgtccgta ccttcttccg tggcgttgat attagtcagg tgttcagtgc
     5641 gaaagatatt gcccgccgga caccgaatgg cttaattcgt aaaactcctg gcttcggccc
     5701 acgttcggca ctgaaattat tgcaggatga agatcgcgaa tatggctacg ttatcgaacg
     5761 cggtaataag tttgtcggcg cagtctccat cgattcgctt aaaaccgcgt taacgcagca
     5821 gcaaggtctt gatgcggcgc tgattgatgc gccgttagca gtcgatgcac aaacgcctct
     5881 tagcgagttg ctctctcatg tcggacaggc accctgtgcg gtgcccgtgg tcgacgagga
     5941 ccaacagtat gtcggcatca tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg
     6001 ggtaaataat ggctgatcaa aataatccgt gggataccac gccagcggcg gacagtgccg
     6061 cgcaatccgc agacgcctgg gggacaccga cgactgcacc ggctgacggc ggtggtgctg
     6121 actggctgac cagtacgcct gcgccaaacg tcgagcattt taatattctc gatccgttcc
     6181 ataaaacgct gatcccgctc gacagttggg tcactgaagg gatcgactgg gttgttaccc
     6241 atttccgtcc cgtcttccag ggcgtgcgcg ttccggttga ttatatcctc aacggtttcc
     6301 agcaattgct gctgggtatg ccagcgccag tggcgattat cgttttcgct ctcatcgcct
     6361 ggcagatttc cggggtcgga atgggcgtgg cgacgctggt ctcgctgatt gccatcggcg
     6421 caatcggtgc ctgttcgcag gcaatggtga ctctggcgct ggtgttaact gccctgctgt
     6481 tctgtatcgt catcggtttg ccgttgggga tatggctggc gagaagtccg cgagcggcga
     6541 aaattattcg tccactgctt gatgccatgc agaccacgcc agcgtttgtt tatctggtgc
     6601 caatcgtcat gctatttggt atcggtaacg tgccgggcgt ggtggtgacg atcatctttg
     6661 cgctgccgcc gattatccgt ctgaccattc tggggattaa ccaggttccg gcggatctga
     6721 ttgaagcctc gcgctcattc ggtgccagcc cgcgccagat gctgttcaaa gttcagttac
     6781 cgctggcgat gccgaccatt atggcgggcg ttaaccagac gctgatgctg gccctttcta
     6841 tggtggtcat tgcctcgatg attgccgtcg gcgggctggg tcagatggta cttcgcggta
     6901 tcggtcgtct ggatatgggg cttgccaccg ttggcggcgt cgggattgtg atcctcgcca
     6961 ttatcctcga tcgcctgacg caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc
     7021 gctggtacac cactggccct gttggtctgc tgacccgccc attcattaag taactctgca
     7081 cttgcccggt gacgccgggc attatcaccc tgccaaaaaa aggaataaca atgcgacata
     7141 gcgtactttt tgcgacagcg tttgccacgc ttatctctac acaaactttt gctgccgatc
     7201 tgccgggcaa aggcattact gttaatccag ttcagagcac catcactgaa gaaaccttcc
     7261 agacgctgct ggtcagtcgt gcgctggaga aattaggtta taccgtcaac aaacccagcg
     7321 aagtggatta caacgttggc tacacctcgc ttgcttccgg cgatgcaacc ttcaccgccg
     7381 tgaactggac gccactccat gacaacatgt acgaagctgc cggtggcgat aagaaatttt
     7441 atcgtgaagg ggtatttgtt aacggcgcag cacagggtta cctgatcgat aagaaaaccg
     7501 ccgaccaata caaaatcacc aacatcgcac aactgaaaga tccgaagatc gccaaactgt
     7561 tcgataccaa cggcgacggt aaagcggatt taaccggttg taaccctggc tggggctgcg
     7621 aaggtgcgat caaccaccag cttgccgcgt atgaactgac caacaccgtg acgcataatc
     7681 aggggaacta cgcagcgatg atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg
     7741 tgttttatta cacctggacg ccgtactggg tgagtaatga gctgaagcca gggaaagatg
     7801 tggtctggtt gcaggtgccg ttctccgcac tgccgggcga taaaaatgcc gataccaaac
     7861 tgccgaatgg cgcgaattac ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag
     7921 cctggaccga gaaaaatccg gcggcagcca aactgtttgc cattatgcag ttgccagtgg
     7981 ccgatattaa cgcccagaac gccattatgc atgacggcaa agcctcagaa ggcgatattc
     8041 agggccacgt tgatggctgg atcaaagccc accagcagca gttcgatggc tgggtgaatg
     8101 aggcgctggc agcgcagaag taatttttat tcgggcggat aaggcgtttt cgcctcatcc
     8161 gccgctctgt acacaatgcc tgatgcgacg ctgacgcgtc ctatcatgcc tacaaaccac
     8221 atcgcacatt tcaacaagct atctttcatc ccatattcat caacatccgc tattattgat
     8281 ttccagctta atcatcacct gatgaacaaa aataatgact aaacctaatc atgagcttag
     8341 cccggcgctg atcgtgctga tgtctatcgc caccggtctg gcggtagcca gtaactatta
     8401 cgcccagcca ttgctcgaca ccatcgcgcg taacttttcc ctttccgcca gttcggcagg
     8461 ctttattgtt accgccgcgc agttgggcta tgccgcaggt ctactgtttc ttgttcccct
     8521 cggtgatatg tttgaacgcc gccgcctgat tgtctcgatg accttactgg cagcgggcgg
     8581 tatgttaatc actgccagca gtcagtcact ggcgatgatg atcctcggta cggcattaac
     8641 cggtttattc tcagtcgtgg cacaaattct ggttccgctg gcagcgacgc tggcttcacc
     8701 ggataaacgc ggcaaagtgg ttggcaccat tatgagcggt ctgctgttgg ggatattgct
     8761 ggcacgaacc gttgccgggt tactggcaaa cctcggcggc tggcgcaccg tcttttgggt
     8821 tgcttcggtg ttaatggcac tgatggcgct ggcattatgg cgtggcctgc cacaaatgaa
     8881 atcagaaacc cacctcaact acccacagtt gttgggttcc gttttcagca tgttcatcag
     8941 cgataagatc ctgcgcaccc gcgcgttgct gggctgcatg acctttgcca acttcagcat
     9001 tctctggacc tcaatggcct ttttgcttgc cgctccacct tttaactaca gcgatggtga
     9061 cattggtctg tttgggcttg cgggagctgc cggagcgttg ggcgctcgtc cggcgggcgg
     9121 ttttgccgac aagggcaaat cgcaccacaa ctttcggtct gcttctgctg ttactttcat
     9181 ggctggcaat ctggtttggt cacacttccg tactggcgtt gattatcggc attctggtgc
     9241 tggacctcac cgtacagggc gtgcatatca ctaaccagac ggtaatttat cgaatacatc
     9301 ctgatgcgcg taatcgcctg accgcaggtt acatgaccag ctactttatt ggcggtgccg
     9361 ccggttcgct aatttcagcc tcagcctggc aacatggcgg ttgggctggc gtttgtctgg
     9421 ctggcgcgac gattgccctg gttaacttac tggtctggtg gcgaggtttt catcgtcagg
     9481 aagccgcaaa ttaagcaatc agcaaccgtt ttcgtggctt tacacttata agggtgttaa
     9541 gaagcccatc agtctgataa ggttaagata ttcattcagt ctatttataa tattaacaat
     9601 cgttaagcgt acactctatg gaaagcccta ctccacagcc tgctcctggt tcggcgacct
     9661 tcatggaagg atgcaaagac agtttaccga ttgttattag ttatattccg gtggcctttg
     9721 cgttcggtct gaatgcgacc cgtctgggat tctctcctct cgaaagcgtt tttttctcct
     9781 gcatcattta tgcaggcgcg agccagttcg tcattaccgc gatgctggca gccgggagta
     9841 gtttgtgggt tgctgcactg accgtcatgg caatggatgt tcgccatgtt ttgtatggcc
     9901 cgtcactgcg tagccgtatt attcagcgtc tgcaaaaatc gaaaaccgcc ctatgggcgt
     9961 ttggcctgac ggatgaggtt tttgccgccg ccaccgcaaa actggtacgc aataatcgcc
    10021 gctggagcga gaactggatg atcggcattg ccttcagttc atggtcatcg tgggtatttg
    10081 gtacggtaat aggggcattc tccggcagcg gcttgctgca aggttatccc gccgttgaag
    10141 ctgcattagg ttttatgctt ccggcactct ttatgagttt cctgctcgcc tctttccagc
    10201 gcaaacaatc tctttgcgtt accgcagctt tagttggtgc ccttgcaggc gtaacgctat
    10261 tttctattcc cgtcgccatt ctggcaggca ttgtctgtgg ctgcctcact gcgttaatcc
    10321 aggcattctg gcaaggagcg cccgatgagc tatgaggttc tgctgcttgg gttactggtt
    10381 ggcgcggcga attattgctt ccgctatttg ccgctgcgcc tgcgtgtggg taatgcccgc
    10441 ccaaccaaac gtggcgcggt aggtattttg ctcgacacca ttggcatcgc ctcgatatgc
    10501 gctctgctgg ttgtctctac cgcaccagaa gtgatgcacg atacacgccg tttcgtgccc
    10561 acgctggtcg gcttcgcggt actgggtgcc agtttctata aaacacgcag cattatcatc
    10621 ccaacactgc ttagtgcgct ggcctatggg ctcgcctgga aagtgatggc gattatataa
    10681 cccacaagaa tcatttttct aaaacaatac atttacttta tttgtcactg tcgttactat
    10741 atcgactgaa attaatgagg tcatacccaa atggatagtt cgtttacgcc cattgaacaa
    10801 atgctaaaat ttcgcgccag ccgccacgaa gattttccta atcaggagat ccttctgact
    10861 cgtctttgca tgcacatgca aagcaagctg ctggagaacc gcaataaaat gctgaaggct
    10921 caggggatta acgagacgtt gtttatggcg ttgattacgc tggagtctca ggaaaaccac
    10981 agtattcagc cttctgaatt aagttgtgct cttggatcat cccgtaccaa cgcgacgcgt
    11041 attgccgatg aactggaaaa acgcggttgg atcgaacgtc gtgaaagcga taacgatcgc
    11101 cgctgcctgc atctgcaatt aacggaaaaa ggtcacgagt ttttgcgcga ggttttacca
    11161 ccgcagcata actgcctgca tcaactctgg tccgcgctca gcacaacaga aaaagatcag
    11221 ctcgagcaaa tcacccgcaa attgctctcc cgtctcgacc agatggaaca agacggtgtg
    11281 gttctcgaag cgatgagcta acgcgtcatc tcgctcaaaa atccagattt ataaaagaaa
    11341 aaatgaccgg ccagcatcgc aacatgctgg cctttttggc aagcaggtcg gctcagccga
    11401 tgagttaaga agatcgtgga gaacaatatg agcgcaaatg cggagactca aaccccgcag
    11461 caaccggtaa agaagagcgg caaacgtaag cgtctgctcc tccttctcac cttgctcttt
    11521 ataattattg ccgtagcgat agggatttat tggtttttgg tactgcgtca tttcgaagaa
    11581 accgatgacg catacgtggc agggaatcag attcaaatta tgtctcaggt gtctggcagc
    11641 gtgacgaaag tctgggccga taacaccgat tttgtaaaag aaggcgatgt tctggtcact
    11701 ctcgacccga cagatgctcg ccaggcgttt gaaaaagcca aaactgcact ggcttccagc
    11761 gttcgccaaa cccaccagct gatgattaac agcaaacagt tgcaggcgaa tattgaggtg
    11821 caaaaaatcg ccctcgcgaa agcacaaagc gactacaacc gccgtgtgcc gctgggcaat
    11881 gccaacctga ttggtcgcga agagctgcaa cacgcccgcg acgccgtcac cagtgcccag
    11941 gcgcaactgg acgtcgctat tcaacaatac aatgccaatc aggcgatgat tctggggact
    12001 aaactggaag atcagcctgc cgtgcaacag gctgccaccg aagtacgtaa cgcctagctg
    12061 gcgctggagc gtacccgtat tgtcagtccg atgaccggtt atgtttcccg tcgtgccgtg
    12121 caaccgggtg cgcaaattag cccaacgacg ccgctgatgg cggtcgttcc agccaccaat
    12181 atgtgggtgg atgccaactt taaagagacg cagattgcca atatgcgcat cggtcagccg
    12241 gtcaccatca ccaccgatat ttacggcgat gatgtgaaat acaccggtaa agtggttggt
    12301 ctggatatgg gcacaggtag cgcgttctca ctgcttccgg cgcaaaatgc gaccggtaac
    12361 tggatcaaag tcgttcagcg tctacctgtg cgtatcgaac tggaccagaa acagctggag
    12421 caatacccgc tgcgcattgg tttgtccacg ctggtaagcg tcaataccac taaccgtgac
    12481 ggtcaggtac tggcaaataa agtacgttca acaccggtcg cggtaagtac cgcgcgtgaa
    12541 atcagcctag cacctgtcaa taaactgatc gacgatatcg taaaagctaa cgctggctaa
    12601 tccagaggtg cgtgtgatgc aacagcaaaa accgctggaa ggcgcgcaac tggtcattat
    12661 gacgattgcg ctgtcactgg cgacattcat gcaggtgctg gactccacca ttgctaacgt
    12721 ggcgatcccc actatcgccg ggaatctggg ctcatcgctc agccaaggaa cgtgggtaat
    12781 cacttctttc ggggtggcga atgccatctc gatcccgctt accggctggc tggcaaagcg
    12841 cgtcggggaa gtgaaactgt tcctttggtc caccatcgcc tttgctattg cgtcgtgggc
    12901 gtgtggtgtc tccagcagcc tgaatatgct gatcttcttc cgcgtgattc aggggattgt
    12961 cgccgggccg ttgatcccgc tttcgcaaag tctattgctg aataactacc cgccagccaa
    13021 acgctcgatc gcgctggcgt tgtggtcgat gacggtgatt gtcgcgccaa tttgcggccc
    13081 gatcctcggc ggttatatca gcgataatta ccactggggc tggatattct tcatcaacgt
    13141 gccgattggc gtggcggtgg tgttgatgac actgcaaact ctgcgcggac gtgaaacccg
    13201 caccgaacgg cggcggattg atgccgtggg gctggcactg ctggttattg gtatcggcag
    13261 cctgcagatt atgctcgacc gcggtaaaga gctggactgt ttttcatcac aggaaattat
    13321 cattcttacc gtggtggcgg tggtggctat ctgcttcctg attgtctggg agctgaccga
    13381 cgataacccg atagtcgatc tgtcgttgtt taagtcgcgc aacttcacca tcggctgctt
    13441 gtgtatcagc ctcgcgtata tgctctactt cggcgctatt gttctgctgc cgcagttgtt
    13501 gcaggaggtc tacggttaca cggcgacctg ggcaggtttg gcctctgcgc cggtagggat
    13561 tattccggtg attctgtcgc cgattatcgg ccgcttcgcg cataaactgg atatgcggcg
    13621 gctggtaacc ttcagcttta ttatgtatgc cgtctgcttc tactggcgtg cctatacctt
    13681 tgaaccaggt atggattttg gcgcgtcggc ctggccgcag tttatccagg ggtttgcggt
    13741 ggcctgcttc tttatgccgc tgaccaccat tacgctgtct ggtttgccac cggaacgact
    13801 ggcggcggca tcgagcctct ctaactttac gcgaacgctg gcggggtcta tcggcacgtc
    13861 gataaccacg accatgtgga ccaaccgcga gtcgatgcac catgcgcagt tgactgagtc
    13921 ggtaaacccg ttcaacccga atgcccaggc gatgtacagt caactggaag ggcttgggat
    13981 gacgcaacag caggcatcag gctggattgc ccagcagatc accaatcagg ggctgattat
    14041 ttccgccaat gagatcttct ggatgtcagc cgggatattc ctcgtactgc tggggctggt
    14101 gtggtttgct aaaccgccat ttggcgcagg tggcggcgga ggcggtgcgc actaagtaca
    14161 actaagccag ttcatttgaa ctggcttttt tcaattaatt gtgaagatag tttactgact
    14221 agatgtgcag ttcctgcaac ttctctttcg gcagtgccag ttcttcgttg ctgttgatgc
    14281 gtacgtcacg ttccagaatg ctacgcgcaa tatcctgcgc ttcctgcaac gagtgcatct
    14341 ggtaagtgcc acactggtag acgttcagtt ccgggatctg attctgatcc tgcactttca
    14401 gcacgtcttc cattgccgct ttccaggcat cagcaacacg ctgctcatct ggcgtaccaa
    14461 tcagactcat ataaaaaccg gtgcggcagc ccattggcga gatatcgata atctctacac
    14521 cattaccgtt aagatggtta cgcataaaac cagcaaacag gtgctccagg gtatggctcc
    14581 ctctttctgg catcacttct ttgttcggca cgcagaagcg cagatcgaac acggtgattg
    14641 cgtcgccatg cggggtgttc attgttttcg ccacccgaac tgcaggcgct tccatccggg
    14701 tatgatcgac tgtgaagcta tctaacaacg gcatttagcc acctccggta atttttttaa
    14761 aaattttctg aactctttct tcccaggcga gtctgagtat atgaaagacg cgcatttgtt
    14821 atcatcatcc ctgaattcag agatgaaatt ttggccactc acgagtggcc tttttctttt
    14881 ctgtcaggcg tgtttttcca gccacaccgc aaacggttcg gtatcagcgg tttccatttc
    14941 ctgctgacgg cgttcagatg cctcgcgctc ggctacaaaa tcctcttcgc gcagaatttc
    15001 cagcggctct tcacgcagca gattacggta ggcttctgca aacgctttgc ctgttccgcc
    15061 aataccagta tcaatcatag accttaagat acgggcagag aaagtcagat cgggattatc
    15121 gaagcaggca accagttcat cacacacttt ctgatacgct tcgccgccgt taatgctatc
    15181 cagcgtttgc gcgacgcgtt tcagatcgtg gaacagatct ttacccacct gcagtaacgg
    15241 gaactgcgcg gtttcgcagc cgatacccag cgtcaggccc ggtttgcgac cttcaaggat
    15301 cacccggttc cagttaacgc gcgtacaggc aagttcgcta ctgctcattt ccggtgcatc
    15361 agccagcgca caccagacca taaacaggtc gaggaatcgc acctgctgtt catctacacc
    15421 aatcggcgag aacgggttga tgtccagcga acgcacttca atatattcaa tgccgccacg
    15481 taacagcgca tcagaaggtg actcgccgct gcgggtaacg cgttttggac gaatcggcgc
    15541 atacagttcg ttttcaatct gcagcacgtt gctattgatt tgcagcctct taccgtcttt
    15601 ctcaatacca atcttcgcgt actcttccga tggcgttttg attgcctgtt taaggcccgc
    15661 tacgtactcg taaagatcgt tgaaggtaat accaagattg ctttgcgatt tattggtata
    15721 gccgagatcg ctcaaacgaa gagaggtcgc atacggcagg taatacatac cgcactcggt
    15781 tttctcaaac ggcagcgacg ttggttttcc ttgcaggaaa gaagaacaaa tcgccggaga
    15841 tgcaccaaac agataaggaa tgacccaacc gaaacgatag taattgcgga taacgcggaa
    15901 atagcccgca gaaattttct ctttggcatc agcgcccgag atatcaccgc acttcgcttg
    15961 ccagaatgcc attggcaaag agaaattgta gtgcacgccg gaaatggttt gcatcagcgc
    16021 gccgtagcga tttttcagcc cttcacgata cagcgtttta aagcgtccgg tgttagaagt
    16081 gccgtactgt gccagttcga tgtcctgacc ttctgcgatg tagcatggca tacttaacgg
    16141 ccacatccgc tcatcgccca tattgcgcgc cgtataacga tgcagatcgc gcataaaggt
    16201 cagcatatgt tcaatatcac catccactgg tgtaatgaat tccagcaatg cttccgcaaa
    16261 atcggtagta atccatttgt gcgtcagtgc ggaacctaat gcttcaggat gacctgttgt
    16321 tgccagtgtg ccatcagcat taacacgcaa agtttcgcgc tccagcccac gctgtatccc
    16381 ctttaacgcc tgaggatgtt tttccagcca ggccagcgcc tgtgatacgt ccgggatcaa
    16441 attgacctcc cgcctgtcaa aatcgtttta attagcataa ctgtaatggt gaccatatgt
    16501 gcaggcctac aattagtgcc accacatcat gccctgaacg gtcgctgctg caactgcaac
    16561 atagcgtaac gctttaccaa ggcacaaaaa aaagattact ggcccccagg agatgcgcat
    16621 ccatcccgct aacagacaca gtaaatcgcc aaccacgggc atccagctta ataatagcgt
    16681 gactgcacca tagcgtttca gccagccggt agctttctct tgccagcgcg atgttttacg
    16741 caatggaaag aaacgcccaa ggataacgtt agttaaccct ccaaggctat tacccattgt
    16801 tgctgttaaa actaaaaccc agggatgact gatcccggaa agcaacattg ccaccagcac
    16861 gacttcggag ttgccgggta atagtgtagc gctgagaaaa ctactggcaa acaacgagaa
    16921 aagcgataac gcttcactca cagcaagcga acatccacag cgtccatgcc tgctgcacgg
    16981 gccgcctgaa taccgaaatc ggcatcttca aagaccacac actgcgtcgg ttgcacgccc
    17041 atacgctgcg cgcacaacaa aaatgtgtct ggcgcgggtt tatggtgttt gacgtgatca
    17101 gcagcgacga cggcgtcaaa ataacggcgt aatcccaggt gcgccagcaa tgcctctgcg
    17161 atggcgcttt cactccccgt tcctacagcc attgggcgac gaccatgcca acttttcacc
    17221 acttcaacaa gaggaagcgg ctcgacacta tccagcagca tacttcttac tgcttctgtt
    17281 ttttcacgcg ctaacgcatg cgggtcgaga tcggcctgat tcagctcaat aattgcctga
    17341 gcaatacgcc aggtgggcga tccattaagc gcaatcatcg cctgaatatc gtactgaaga
    17401 ccgtagtgcc ctaatacttc gcgccacgct ttacggtgcg taggctccgt atccaggatt
    17461 gtgccatcca tatcaaaaat taaacctgca taacgctcgt acatggtctt ctcgcaagtc
    17521 gaaaaatatg acgctacttt agcgtaattg cttgattttg tcgctgatgg cgaaggagaa
    17581 gaaaattcag gaaatggata aagtagttat ttagaaatga gatatttttg aaggaaattt
    17641 tttggagaat atggtgcatc cgggaggatt cgaacctccg accgctcggt tcgtagccga
    17701 gtactctatc cagctgagct acggatgcat cgggaaactt actttactgc aggttttttg
    17761 ataccgctac taaagccgta tcaagtaaga gatggtgcat ccgggaggat tcgaacctcc
    17821 gaccgctcgg ttcgtagccg agtactctat ccagctgagc tacggatgca aatggcggtg
    17881 aggcggggat tcgaaccccg gatgcagctt ttgaccgcat actcccttag caggggagcg
    17941 ccttcagcct ctcggccacc tcaccacacg cctcttacga gtgcttcgaa gaacttgttt
    18001 atcgctcatc gtcgctgcgt ggcgcacata ttactttctg agacttataa gtcaaacaat
    18061 ttttcccaca cttttatcgt ttgcacactt cacgctcaat tagtctgtaa aaacgtcaaa
    18121 aagagtgttt tatctacaga aaaacggagg tctggcagat agcagtaatg caaaaaaatg
    18181 gagacttaag ttgaatgaac gggagtaaag cgaaaagact atagagtgaa ggagaaattt
    18241 tgagggtgcg tctcaccgat aaagatgaga cgcggaaaga ttagtaactg gactgctggg
    18301 atttttcagc ctggatacgc tggtagatct cttcacggtg aacagaaact tccttcgggg
    18361 catttacgcc aatacgtacc tggttgccct ttacccctaa aactgtcacg gtgacctcat
    18421 ccccaatcat gagggtctca ccaactcgac gagtcagaat cagcattctt tgctccttga
    18481 aagattaaaa gagtcgggtc tctctgtatc ccggcattat ccatcatata acgccaaaaa
    18541 gtaagcaacg acaaacacat tacatgtaag cagtcatggc attacattct gttaaaccta
    18601 agtttagccg atatacacaa cttcaacctg actttatcgt tgtcgatagc gttaatgcga
    18661 atgccgtgaa gcgagtccac ggcattgcct gacgcttata ttattgcaat ttcgcgctga
    18721 cccagccttt cacactggct aacgctgcag gtaaggccgc agcatccgta ccaccggctt
    18781 gcgccatgtc aggacgtcca ccacccttgc cgcccacctg ctgagcgacc ataccaatca
    18841 gttcccctgc tttcacacga tctgtgacgt ccttagatac gcctgcaatc agagaaacct
    18901 taccttcggc taccgttgcc agcacgataa ttgtcgaccc cagctgattt tttaaatcgt
    18961 caaccatggt acgcaacatt ttcggctcaa caccgctaag ctcgctaacc aacagcttaa
    19021 caccattaac atcaattgcc ttactggaaa gatttgcgct ctcctgtgcg gcagcttgtt
    19081 ctttaagctg ttgtagttct ttttctaact gacgcgtacg ttccagtact gagcgcactt
    19141 tatccgccag attattgcta tcgcctttca gcagatgcgc gacttcgctt aagcgatcgc
    19201 tgtctgcatg aacggtggtg atagcgcctt ctccggttac cgcttcgata cgacgaacgc
    19261 ctgcagcagt acccgattca gagatgatgc ggaacagacc aatatcacca gtgcggctgg
    19321 cgtgagtacc gccacacagc tcggtggaga aatcgcccat gctcagcacg cgtacacgct
    19381 catcatactt ctcgccgaac agcgccatcg cacctttcgc tttcgccgct tcgagattca
    19441 tgatgttggt ttcgatcggc aaattgcgac gaatctgtgc gttcaccagg tcttcgaccg
    19501 cacgaatctc ttccggtttc atcgcttcgt tgtgtgagaa gtcgaagcgc agcaccttgt
    19561 cgttaaccag tgaacctttc tgcgatacat gagtccccag aacctggcgc agcgcagcgt
    19621 gcatcaggtg cgttgcggag tgattcagac gaatacgggc gcgacgagcc tcatcaatat
    19681 cagcctgcac agcgtcgccc actttcagag aacccgcagc aagtttaccg atgtgaccaa
    19741 tcgcctggcc gtatttctgc gtatcttcca ccgcgaagga gaagttagcg cctttcagtt
    19801 cgcctttatc accaacctga ccgccggatt ccgcatagaa tggcgtttga tccagcacga
    19861 ccacagcttc ctggcctgca ttgatggcat caaccgcttt accatcaaca aacagcgcgg
    19921 tcactttgcc gttcagttcc agatggtcat agcctttaaa ttcagatgca ctatcaacac
    19981 ggatcattgc gttgtaatcg gcaccaaagc cgctggcttc gcgcgcgcga cgacgctgct
    20041 cttccattgc tgcttcaaaa ccagcttcgt caactttgat gttgcgctca cgacaaacat
    20101 cagccgtcag gtcaaccggg aagccatagg tgtcgtacag acggaaagcc gtttcaccat
    20161 ccagcgtatc accagaaagt tttgccagct cttcatccag caacgccaga ccgcgctcca
    20221 gagtacgggc aaactgctct tcttcagtct tcagcacctg ctcaacctgc gcctgctggc
    20281 gtttcaggtc ttcacccgca gagcccataa cgtcgatcag cggaccaacc agtttgtaga
    20341 agaaggtttc tttcgcgccg agcatattgc cgtgacgcac tgcgcgacga atgatacgac
    20401 gcagtacata accacggttt tcattggacg gcattacgcc atccgcgatc aggaacgcac
    20461 aagaacgaat gtggtcagcg attacgcgca gcgatttatt gctcagatcg gtcgcgccag
    20521 tgactttcgc taccgcctgg atcaacgtgc ggaacaggtc gatgtcatag ttagagttaa
    20581 cgtgttgcag caccgcagca atacgctcca gacccatacc ggtatctaca gacggcttcg
    20641 gcagcggttc catcgtgcca tctgcctggc ggttgaactg catgaagacg atgttccaga
    20701 tctcaatgta gcggtcgccg tcttcttccg gacttcccgg agggccaccc caaatgtggt
    20761 cgccgtgatc gtagaagatt tcggtgcacg ggccgcacgg accagtgtca cccatctgcc
    20821 agaagttgtc agatgcgtat ggcgcacctt tgttatcgcc gatgcgaata atacgttcgc
    20881 gcgggatccc gacttctttt tcccagattt cgtaggcttc gtcgtcgctt tcatagacgg
    20941 taacccacag acgctctttc ggcagggcaa accatttttc gctggtcagc agttcccatg
    21001 caaactgaat ggcatcgtgt ttgaaatagt cgccgaagct gaagttgccc agcatttcga
    21061 agaaggtatg gtgacgcgcg gtgtaaccga cgttttccag gtcgttgtgt ttaccacccg
    21121 cacgcacgca gcgttgggaa gtggtagcgc gggaataatt acgcttgtcg agcccaagga
    21181 acacatcctt gaactggttc atcccggcgt tggtaaacaa caaagttggg tcgttatggg
    21241 gtaccaggga gctgctggca actacctgat gtcccttact atggaaaaag tcgagaaacg
    21301 cctgacggat ctcagcggtg ctcttgctca taattatcct gaaatcaagc taacgaaata
    21361 tcgccaccag ctccagcgtg tcttaaccgc cgggctggta actgaaaagt gggaataaga
    21421 taagttttct tgactgggaa gtaaaatacc gtatgcgttc agtcggcaaa atttcgccaa
    21481 atatcctgga tatcttccat cagatagcca cgatagagca gaaaacgctg gatcttaact
    21541 ttttctgaaa agacagttgg caaaggttca ccatattttc gcgtcgcctg atcgcgcgcc
    21601 agtgcgcacc agtcgatgtc acattcacgc atcgcttttt ctgtcgcttc gcgggaaata
    21661 cctttctgat tcagttcctg acgaatacgt gcaggtccat aacctttgcg gctacggctg
    21721 gcgataaagc gcgcaacaaa tcggctgtca tcgagatagc catgttcatg gcaccaggca
    21781 ataacgcgct cgtaatcttc tgccgtagca tcaatctctt ctgggccatt tttgcccata
    21841 atcggtgccg cgagtttacg tcgcagttct tgctcactgt gatcgcgcac cgccagaatg
    21901 cgtaccgcac gatccaacag gcgagcatat gccgggcgac gggatgttga ttctgtcatg
    21961 gcatatcctt acaacttaaa aaagcaaaag ggccgcagat gcgacccagg ttttgctgat
    22021 aaacagattt aatagtcaat gagtatgcag aatgttcccg caggcccaat tcgttagagg
    22081 atcgaggtga gagtaatttt tcacgccagt gctggcctgt tcccctcacc ctaaccctct
    22141 ccccaaaggg gcgaggggac cgtccgtgca cacattatct attatcatca ataacgaacg
    22201 attaaaaatc ttcgttagtt tctgctacgc cttcgctgtc atctacagag aaatccggcg
    22261 ttgagttcgg gttgctcagc agcaactcac gtactttctt ctcgatctct ttcgcggttt
    22321 ccgggttatc tttcagccag gcagttgcat tcgctttacc ctgaccgatc ttctcacctt
    22381 tgtagctgta ccacgcgcct gctttctcga tcagcttctc ttttacgccc aggtcaacca
    22441 gttcgccgta gaagttgata ccttcgccgt agaggatctg aaattcagcc tgtttaaacg
    22501 gtgcagcgat tttgttcttc accactttca cgcgggtttc gctacccacc acgttttcgc
    22561 cctctttcac cgcgccgata cgacggatgt cgagacgaac agaggcgtag aatttcagcg
    22621 cgttaccacc ggtagtggtt tccgggttac cgaacatcac accaattttc atacggatct
    22681 ggttgatgaa gatcagcagc gtgttggact gcttcaggtt acccgccagc ttacgcatcg
    22741 cctggctcat catacgtgcc gcaaggccca tgtgagagtc gccgatttcg ccttcgattt
    22801 ccgctttcgg cgtcagtgcc gccacggagt caacgacgat aacgtctact gcgccagaac
    22861 gcgccagggc gtcacagatt tccagtgcct gctcgccggt atccggctgg gagcacagca
    22921 ggttatcgat atcgacgccc agtttacgtg cgtagattgg gtccagcgcg tgttcagcat
    22981 cgataaacgc acaggtttta ccttcacgct gcgctgcggc gatcacctgc agcgtcagcg
    23041 tggttttacc ggaagattcc ggtccgtaga tttcgacgat acggcccatc ggcagaccac
    23101 ctgccccaag cgcgatatcc agtgaaagcg aaccggtaga gatggtttcc acatccatgg
    23161 aacggtcttc acccaggcgc atgatggagc ctttaccaaa ttgtttctca atctggccca
    23221 gtgctgccgc caacgctttc tgtttgtttt cgtcgatagc catttttact cctgtcatgc
    23281 cgggtaatac cggatagtca atatgttctg ttgaaacaat tatactgtat gctcatacag
    23341 tatcaagtgt tttgtagaaa ttgttgccac aaggtctgca atacatacgc agtagcctga
    23401 cgacgcaccg catcacggtc gccgctgaag cattcccgcc gggtaatgcc ttcaccgcgg
    23461 gcagtggcaa aagcaaacca gacggtgccg acaggcttct cttcactgcc gccatccggc
    23521 ccggcgatac cactaataga cacggcataa tcagcacgag ccgctttcag tgcacctatc
    23581 gccatttcca ccacgacggg ttcactcacc gcgccatgct gcgccagcgt ctcttcgcgt
    23641 acgccgatca tctgcgcttt ggcttcgtta ctgtaggtga caaatccgcg ttcaaaccag
    23701 gcggagctac cggcaatatc ggtaatcact ttcgctaccc aaccaccggt acaagactcg
    23761 gcagttgtta cggttgcgcc acgggctttc agcgcctgcc caacctgttc gcttaactgc
    23821 atcagttcac tgtcagtcat cacaacctct gtcagtcaaa aatttatgcc ggacaagata
    23881 gcactttcgt caggaagatg gtgttggcgt tgcaatttta cgaggaggat tcagaaaaaa
    23941 gctgattagc cagagggaag ctcacgcccc cctcttgtaa atagctactg tactcgcgcc
    24001 agcgccacgg cttgtcctaa ctgccagacc gccattgcgt aatgggtgct gtggttgtaa
    24061 cgggtgatgg tgtagaagtt cggcagaccg taccagtact ggtagccggt gccaacatcc
    24121 agacgcagca ggctggcttg ttgatggttg cccagcggct gctgtggcgt taaacctgcg
    24181 gcggcaagct gcgaaatgct gtacttagtt ttgaaaccat ttggcaagcc tggagcctga
    24241 ccgtttgcca ttatcgcgac ctgatcgcct ttcacccagc cgtgcgcttt gaaatagttc
    24301 gccacgctac cgatcgcatc aaccggatcc cacaagttga tatgcccgtc gccgctgaaa
    24361 tctaccgcat attgtttgta agacgacggc ataaactgtc cgtagcccat cgccccggca
    24421 aaggaacctt tcagattgag cggatcgtcc tgctcgtcgc gcgccatcag caggaaggtt
    24481 tccagctcgc cagagaaata ctccgcgcgg cgtgggtagt taaatgacag cgttgccagc
    24541 gcatcgagga tgcgagtttt ccccatcacg cgcccccagc gggtttcaac gccgataatc
    24601 ccgacgataa tttccggcgg tacgccatac acctgccacg cgcgattcaa cgcatcttca
    24661 tactgattcc agaaaaccac accgttctgc acgttgtccg gcgtaataaa ttttttgcga
    24721 taacgcagcc atgcgccgtt cggacctgat ggcggtttca ccgatgtggt tggtgcctgg
    24781 ttatccatca gccgcagtac cgaatccaga cgcttcgcct gggagagaat ttcctgcaac
    24841 tgctgacgat cgaaaccgtg tttgttcacc attttgtcga tgaactgctg ggcattcggg
    24901 ttattagcga aatcgccgcc catctgcatc acattgtgct gcggctcaag caggaagccg
    24961 ccagacggcg ttccggtggt cgtttcagtc tcagtaggtt ttggcttgct gctacaggcg
    25021 gcaagcaaca caaaaagggg aagcaatgtt acataacgac gcttgaacat gaggggtcca
    25081 tttaacagat tcaaccaggg gcaagtatgg taaagcatca cgccccgcac aaggaagcgg
    25141 tagtcactgc ccgatacgga ctttacataa ctcaactcat tcccctcgct atacttttat
    25201 tcaaactttc aaattaaaat atttatcttt cattttgcga tcaaaataac acttttaaac
    25261 ctttcaacct gattagatta ggttgccgtt tggtaataaa acaataaatc ctgaaggaga
    25321 gaacaatgat agaaaccatt actcatggtg cagagtggtt tatcgggctg ttccaaaagg
    25381 gcggagaggt gtttaccgga atggtgaccg gcattcttcc gctgttgatt agcctgctgg
    25441 ttatcatgaa cgcactgatt aattttatcg gtcagcatcg tattgaacgt tttgctcaac
    25501 gttgcgccgg taaccctgtt tcccgttacc tactgttacc gtgcattggc acgtttgtct
    25561 tttgcaatcc gatgacctta agcctaggtc gctttatgcc ggaaaagtac aaacccagct
    25621 actacgcggc ggcctcttat agctgccact caatgaatgg cctcttcccc catatcaacc
    25681 ctggcgaact gtttgtttat cttggcattg ccagcggtct gacaacgctg aacctgccac
    25741 ttggcccact ggcggtgagt tatctgctgg ttggtctggt caccaatttc ttccgcggct
    25801 gggtgaccga tctgaccacc gccatttttg agaaaaagat gggcattcaa cttgaacaaa
    25861 aagttcacct ggcaggagca acatcatgac gcgtattcgg atcgaaaaag gaacgggtgg
    25921 ctggggcggc ccgcttgagc tggaagctac gccgggcaaa aaaatcgtct atatcaccgc
    25981 cggtacccgg cctgcgattg ttgacaaact ggcacaactt actggctggc aggctattga
    26041 cggatttaaa gaaggtgaac ccgcggaggc ggaaattggt gtcgcggtaa tcgactgtgg
    26101 cggcacatta cgctgcggca tctatccgaa acgacgtatt cccaccatta atatccactc
    26161 gacgggcaag tccggtccgc tggcgcagta cattgtggaa gatatttatg tctctggcgt
    26221 aaaagaagaa aacatcactg tagtgggtga tgcgacacca caaccctctt ccgtgggccg
    26281 tgactatgac accagtaaga aaatcaccga acaaagcgat ggtttactgg cgaaggtggg
    26341 aatgggcatg gggtccgccg ttgccgtgtt gtttcagtct ggtcgtgaca ccatcgacac
    26401 tgtattaaaa accattctgc cgtttatggc attcgtctcg gcgctcattg gcatcattat
    26461 ggcttctggc cttggtgact ggattgccca cggtcttgct ccgctggcga gccatccact
    26521 gggtctggtc atgctggcgc tcatctgctc cttcccactg ctttcacctt tcctcggccc
    26581 aggcgcagtt atcgcacagg ttatcggcgt attgattggc gtgcagattg gtctcggcaa
    26641 tattccgccg catctggctt taccggcact gtttgccatc aacgcgcagg cggcctgcga
    26701 cttcatcccg gtcggtttgt cgctggcgga agcccgtcag gacacggttc gcgtcggtgt
    26761 cccttctgta ctggtgagcc gctttttaac cggcgcgccg actgtactga tcgcctggtt
    26821 tgtctccggt tttatctatc aatagaggct gaaacatgac cgttatttat cagaccacca
    26881 tcacccgtat cggcgcgagt gccactgacg ccctcagcga ccagatgctc atcacctttc
    26941 gtgaaggcgc gccagcggac ctcgaagagt attgcttcat tcattgccac ggcgagttga
    27001 aaggtgcact ccatcccggt ttgcaatttt cactcgggca gcatcgctat ccggtgaccg
    27061 ctgttggcag catggcggaa gacaaccttc gcgaactggg tcatgtcacc ctgcgcttcg
    27121 atggtttaaa cgaagcggaa tttccgggca ctgtccatgt ggcaggccct gtacccgacg
    27181 atatcgcgcc gggatcggtt ttgaagtttg aatctgttaa ggagtaaaaa atgaatcagg
    27241 ttgccgttgt catcggtggt gggcaaacct taggcgcgtt cctgtgccac ggtctggctg
    27301 ccgaggggta tcgcgtcgcg gttgtcgata ttcagagcga caaagccgca aatgtggcac
    27361 aagaaattaa cgccgaatat ggtgaaggta tggcgtacgg ttttggtgct gacgccacta
    27421 gcgagcaaag cgttctggcg ctctctcgtg gggtagatga aatctttggt cgcgtggatt
    27481 tgctggtcta tagcgccgga atagcaaaag cagcctttat cagcgacttc cagctcggcg
    27541 attttgaccg ttcgctacaa gtgaatctgg tgggttattt cctgtgtgcc cgtgaatttt
    27601 cgcgtttgat gatccgcgac gggattcagg gtcgcattat tcagatcaac tcgaaatccg
    27661 gcaaagtggg cagcaaacac aactctggct acagcgcagc gaaatttggt ggcgtcgggc
    27721 tgactcaatc actggcgctg gatctggcgg agtacggcat tacggtgcat tcactgatgc
    27781 tcggtaacct gctgaaatcg ccgatgttcc agtcactgtt gccacaatac gcgaccaagc
    27841 tgggtatcaa accggatcaa gtcgagcagt attacatcga caaagtaccg ctcaaacgcg
    27901 gctgcgatta tcaagatgtg ctgaatatgc tgctgttcta cgccagtcct aaggcgtcgt
    27961 actgcaccgg acagtcgatc aatgtcaccg gcggtcaggt gatgttctga tcaacagcgg
    28021 agatccgtta aggatctccg tgagactata gaatgcctga tgcgctacgc tcatcaggca
    28081 tacaggactt ccgccactac attaaggaaa agttatggta tccgcactca tcaccgtcgc
    28141 cgttatcgcc tggtgtgcgc aactggcctt aggcggctgg caaatttctc gttttaaccg
    28201 tgccttcgac acactatgcc agcaagggag ggttggcgtg ggccgttcca gcgggcactt
    28261 taaaccgcgg gtcgtggtcg ccatcgcgct ggacgatcag cagtgcatcg tcgacatctt
    28321 gtttatgaaa ggactgactg tcttcgcccg accgcaaaaa attcccgcaa ttaccggcat
    28381 gcatgcgggt gatttacagc ccgatgtgat ctttccccat gatccactat cacagaatgc
    28441 tctatcattg gcgcttaaac tgaaacgtgg ataatttcgt tgtgaatgtt acttgcttgc
    28501 gaagttatca ttttgaaacc taaatcaggt aatcacgccc atgaaacctc gtcagcgtca
    28561 ggccgccatt ctggagtatc tgcaaaagca gggtaaatgc tcggttgaag aattggcgca
    28621 atactttgac accacaggca caaccattcg caaagatctg gtcattctgg aacatgccgg
    28681 aaccgtcatt cgtacttatg gcggagtggt gttgaataaa gaggaatccg atccgcctat
    28741 cgatcataaa acgctcatca acacccacaa gaaagagctg attgcagaag ctgccgttag
    28801 ttttatccat gatggcgatt cgatcattct tgatgctggc agtaccgttt tgcagatggt
    28861 tcccctgctc tcgcgcttta ataacatcac ggtgatgacc aacagcctgc atatcgtcaa
    28921 tgcgctatcc gaactggata acgaacaaac tatcctgatg ccaggcggaa catttcgcaa
    28981 aaaatcggcc tcatttcacg ggcaactggc agagaatgcc ttcgagcatt tcaccttcga
    29041 taaattgttt atgggcaccg acggcatcga tctcaatgcg ggcgtaacca cgttcaacga
    29101 ggtttatacc gtcagtaagg caatgtgcaa tgccgcgcgc gaagtgattt tgatggcgga
    29161 ctcatcaaag tttggccgta aaagccccaa cgtagtttgc agtcttgaaa gcgtcgataa
    29221 gctaattacc gacgcaggta tcgatccggc gtttcgtcag gcgctggaag agaaagggat
    29281 cgatgtgatc ataaccggag agagcaatga gtgaagcact actgaacgcg ggacgtcaga
    29341 cgttaatgct ggagttgcag gaagcaagcc gtttaccgga acgtctgggc gatgattttg
    29401 ttcgcgccgc caatatcatc ctgcactgcg aaggcaaagt ggtggtttcg ggaattggca
    29461 aatcgggcca cattggtaag aaaatcgccg caacacttgc cagtaccggc acaccggctt
    29521 tttttgtcca tccggcagaa gcactgcacg gcgatctggg gatgattgaa agccgcgatg
    29581 tgatgctgtt tatctcttac tccggtggcg cgaaggaact ggatctgatt attccgcgtc
    29641 tggaagataa atctatcgcg ctgctggcga tgaccggcaa accgacgtca ccgctgggcc
    29701 tggcggcgaa agcggtgctg gatatctccg tagaacgcga agcctgcccg atgcaccttg
    29761 cgccgacctc cagcaccgtc aataccctga tgatgggtga cgcgctggcg atggcggtca
    29821 tgcaggcgcg cggttttaat gaagaagatt ttgcccgctc ccacccagct ggggcactgg
    29881 gcgctcgctt gctgaataaa gtgcatcatc tgatgcgtcg tgatgacgct atcccacagg
    29941 tagcgttaac cgccagcgtg atggatgcga tgctggaact cagccgcacc ggtctggggc
    30001 tggtggcggt atgtgacgct caacaacagg tacaaggcgt ctttaccgac ggcgatttac
    30061 gtcgctggct ggtaggcggc ggcgcactca ccacgccagt taatgaagcg atgacgacag
    30121 gcggcaccac attacaggca caaagtcgcg ccatcgacgc caaagaagtt ctgatgaaac
    30181 gcaaaatcac tgccgcacca gtggtagatg aaaacggcaa actcaccggc gcaattaacc
    30241 tgcaggattt ctatcaggcc gggattattt aatccttcaa tcccagacgt ttcgccagcc
    30301 gatgcaggtt ggcgacgtca gtttccagca ttcgcgcaca ggctgcccag ttgtgatgat
    30361 tttgtgccag tgcctggcga atagtttcac gctggaacgc ttctgtcgct tcacgcaggt
    30421 tttgcttaac aacgggcacc gccgccgctt ctggcggcgg caacgtcacc tcaggaaaag
    30481 caaaatgttg cgcctcaaga atcacttcat cgccgttgcg ggtggctctc gccagcacta
    30541 ccacccgatg aatagcatgt tccagttcgc gcacgtttcc cggaaaactg tagtgttgca
    30601 gtaaatttcg cgccccggca cttaatacca cgcgggagag cccctgccgc aaacgacact
    30661 gctcgcagaa ataccccgcc agcagaatga catcatcgcc ccgctcacgc agcggcggca
    30721 ccgaaagtgg aaacacgctc aggcgatgaa acaaatcggc gcggaatcgt cctgccagca
    30781 cctcttcgcg taagtcgcgg ttagtcgccg ccagcacgcg cacatcgacc cgcaaactac
    30841 ggtcatcgcc aacgcgctga atatcgccat actgcaacac cctcagcagc ttggcctgca
    30901 atgccaacga caactcgccg atctcatcga gaaacagagt accgttatcc gccatttcaa
    30961 acttcccgct gcggttactg atagcgccag taaacgctcc tttcacatgc ccgaacaact
    31021 cactttccgc cacactttcc ggcagtgcgg cacagttgag atagaccagc ggattcaccg
    31081 cccgtggcga ggcttcatga atcgctttcg ccaccagctc cttaccggtt cccgtctcac
    31141 cgctgatcag aacgttgaga tcggacgccg ccacaatctc aatctctttt ttcagttgcg
    31201 tcatgccagg ggacaagcca atcatctgcg tctgtttcac cgcttcaaac ggcgtggcat
    31261 cgcctggcag catattctgg ctttccagtt gttcaatcag caacgcattg cttaacgctc
    31321 ccgccgccag cgcggcaatc agccgtaact cttcgtcgct gaaaacatcg aactgatcgg
    31381 gctgcatccc gtcgagcgtc agtgcgccga tcaggttttg tccgacaaac aatggcagac
    31441 caacgcaggc gtgaaccttc agactctcct gcccaggaat caaaccgtca tagggatcgg
    31501 gcaattcgct gtcagcggga aagcgcacca catccccggc gcgggcaatc gcttccagcc
    31561 gtggatgccc ttccagcgca aagcgtctac cgagtacatc ctttgccaga ccgtcgatgg
    31621 caagcggaat aaactgccgc gaatcgtaac gtagcaacgc agacgcatcg cactccagca
    31681 cctgacgtag cgtggtgatc aggcgctgaa aacgatcctg atgaccaatc ccacgctgca
    31741 attcgatggc gatattcgcc agcacatcaa cggaaaaact catctttgcc tcactgtcaa
    31801 tttgactata gatattgtca tatcgaccat ttgattgata gtcattttga ctactcatta
    31861 atgagcataa ttttatttat agagtaaaaa caatcagata agaaactggc acgcaatctg
    31921 caattagcaa gacatctttt tagaacacgc tgaataaatt gaggttgcta tgtctattgt
    31981 ggtgaaaaat aacattcatt gggtgggtca acgtgactgg gaagtgcgtg attttcacgg
    32041 cacggaatat aaaacgctgc gcggcagcag ctacaacagc tacctcatcc gcgaagaaaa
    32101 aaacgtgctg atcgacaccg tcgaccataa attcagccgc gaatttgtgc agaacctgcg
    32161 taatgaaatc gatctggcgg atatcgatta catcgtgatt aaccatgcag aagaggacca
    32221 cgccggggcg ctgaccgaac tgatggcgca aattcccgat acgccgatct actgtactgc
    32281 caacgctatc gactcgataa atggtcatca ccatcatccg gagtggaatt ttaatgtggt
    32341 gaaaactggc gacacgctgg atatcggcaa cggcaaacag ctcatttttg tcgaaacacc
    32401 aatgctgcac tggccggaca gcatgatgac ttacctgaca ggcgacgcgg tgctgttcag
    32461 taacgatgct ttcggtcagc actactgcga cgagcatctg ttcaacgatg aagtggatca
    32521 gacggagctt ttcgagcagt gccagcgtta ctacgccaat atcctgacgc cgttcagccg
    32581 cctggtaacg ccgaaaatta ccgagatcct gggctttaac ttaccagtcg atatgatagc
    32641 gacctcccac ggcgtggtct ggcgcgataa cccgacgcaa attgtcgagc tgtacctgaa
    32701 gtgggccgct gattatcagg aagacagaat caccattttc tacgacacca tgtcgaataa
    32761 cacccgcatg atggctgacg ctatcgccca gggaattgcg gaaaccgacc cacgcgtggc
    32821 ggtgaaaatt ttcaacgtcg cccgaagcga taaaaacgaa atcctgacca atgtcttccg
    32881 ctcaaaaggc gtgctggtcg gcacttcgac gatgaataac gtgatgatgc cgaaaatcgc
    32941 cgggctggtg gaggagatga ccggattacg cttccgtaac aaacgcgcca gtgctttcgg
    33001 atctcacggc tggagcggcg gtgcggtgga tcgcctttcc acgcgcctgc aggatgcggg
    33061 tttcgaaatg tcgctcagcc tgaaagccaa atggcgacca gaccaggacg ctctggagtt
    33121 atgccgtgaa cactgtcgcg aaatcgcccg tcagtgggcg ctcgcgccgc tgccgcagag
    33181 cacggtgaat acggtagtta aagaagaaac ctctgccacg acgacggctg acctcggccc
    33241 acgaatgcag tgcagcgtct gccagtggat ttacgatccg gcaaaaggcg agccaatgca
    33301 ggacgttgcg ccaggaacgc cgtggagtga agtcccggat aacttcctct gcccggaatg
    33361 ctccctcggc aaagacgtct ttgacgaact ggcatcggag gcaaaatgag caacggcatt
    33421 gtgatcatcg gttcgggctt cgccgcccgc caactggtga aaaatattcg caaacaggac
    33481 gccactattc cattaaccct gattgccgcc gacagcatag atgagtacaa caaacccgac
    33541 ctcagccatg ttatcagtca ggggcaacgt gccgacgacc ttacccgcca gacggcgggt
    33601 gaatttgccg ggcagtttaa tctgcgcctg tttccgcaca cctgggtgac ggatatcgat
    33661 gccgaagccc atgtggtgaa aagccagaat aatcagtggc aatacgacaa gttagtgctg
    33721 gcaaccggtg ccagcgcctt tgtcccgcct gtgcctgggc gtgagttaat gctgacgtta
    33781 aatagtcagc aagagtatcg cgcctgtgaa acgcaactgc gggatgcccg acgcgtgttg
    33841 attgtcggcg gtggtttgat tggtagcgaa ctggcgatgg atttttgtcg tgcaggcaaa
    33901 gcggtcacgc taatcgacaa cgctgccagt attctggcgt cattaatgcc accagaagta
    33961 agcagccgct tgcagcatcg gttgacggag atgggcgttc atctgctgtt gaaatctcag
    34021 ttgcaggggc tggaaaaaac ggattctggc attctggcaa cgctggacca ccagcgtagt
    34081 atcgaagtgg atgcggtaat tgccgccacc ggactgcgcc cggaaacagc cctggcacga
    34141 cgcgccgggc tgacgattaa tcgcggcgtt tgcgtcgata gttatctgca aaccagtaat
    34201 gccgatattt acgcgctggg cgattgcgcg gaaattaacg gtcaggtatt gccgttcctg
    34261 cagccgattc aacttagcgg gatggtgctg gcaaaaaatc ttctcggcaa taacacgccg
    34321 ctgaaactac cggcgatgct ggtgaaaatc aaaacgccgg aattaccgct gcatctggca
    34381 ggcgaaaccc agcgtcagga tttacgctgg cagattaata ccgaacgcca gggaatggtg
    34441 gcgcgcggcg ttgacgatgc tgaccagctt cgcgcctttg tggtcagtga ggatcggatg
    34501 aaagaggcat ttgtattgtt gaaaacgttg ccggtgtagg tgggctactg tgcctaaaat
    34561 gtcggatgcg acgctggcgc gtcttatccg acctacgggg acgcatgtgt aggccggata
    34621 aggcatttac gccgcatccg gcaagggtgt ccaaatgcaa cacgctttat ccgttctgga
    34681 cttcacccgc taaccaacgc gccgcagcaa taaccccctg ccccagagac aaaccgccat
    34741 cacccgccgg taaactctgt ggaaagagca atgtgaaatc agcgagataa tgcgccagac
    34801 gtgcacgcag caaacggtta tgaataaccc cgccgctaaa taccagcgta gtgataccac
    34861 gcatcgtggc ctgctcacgc atcaacgcgg caaaaccctg cgccagcgca tcatgaaacg
    34921 cccacgcgcg ttgattaacc ggtgcctgcc agttcagcca ctgctgccag aaagtggcga
    34981 gatccagttg attgtccacc cgcggcatcg tcaccggatg cgtcactccg tcgcatgagg
    35041 ctgcgagcgc ctccagagca caagccgctt caccttcata acttaacgtg gctggcgcac
    35101 agcccagtgc cgccgccact gcatcgaaca accgcccaca cgatgatgcc agcggcgcgt
    35161 taattccacg ctcaatggcc cgcgccagta cgctccagtt ttgctgttgc acacttgccg
    35221 tttcagggta attctgccac tccggcacaa agcgcaggca ctgcgccagc aggtttcgcc
    35281 acggctgctt cgccgccaaa tcgccacccg gaagcgccac tgcaggcaag ccgcccaggt
    35341 gctcacattc gcgatagttc acccgcaggc actcgccgcc ccacaaagcg ccattctccc
    35401 ccataccgat accgtcgagc gtcaaagcaa tgacatcacc gccatccagc ggccactgat
    35461 gctctgccag acacgccgct gcatgggcat gatgatgcag taccgtttgc gtcggcagat
    35521 tcatttcgct cgcccactgg ctggagacat agcccggatg cacatcatgc acaatgtatt
    35581 gcggagtgaa atcgtagatg ttttgcatca ggcgtaacgc ttcgcgccac tgcatctgga
    35641 tgccatcgtc acttaaatcg cccagatgct gactcaacac cgcttgttca ccgcgcacca
    35701 ggcagaaggt atttttcaga tccgcgccaa gacacagcac aggcggaaca tttttaaagc
    35761 ccggaggcaa aaccagcgca tccggcacat acccccgcga acggcgcagc atttcgccgc
    35821 tttcgcgcac cactgaatca tccatccgct gcacgatgtc gcggttatgt agcaagaatc
    35881 cgtcggcaat gccctgcaaa tccgccagcg cctgttcgtt gctgatagct ggtggtttac
    35941 cgctcaggtt gccggaggtc atcaccagcg ggcattgcag ttcctgtaac agcaaatgct
    36001 ggagcgggtt cgcaggcaac atcaccccga cttcgttaag gccaggggcg atatcatcac
    36061 aaagctcagg tacgtatttt ttatccacca gcacaatcgg cgcagcgggc gtggtaagca
    36121 actggcgcgc agcgtctggt aaactttctg ccactggcaa catgactgcc agcggtttcg
    36181 ccgggcgatg tttgcgctcc cgcagtgtcg ccaccgcgtt actgttacgt gcatcgcagg
    36241 caagatgaaa tccgccaatc cctttgatgg cgacaatgtt gcccattttt aactgtgcga
    36301 tagctgcctg taatgccgcc tcttgttctg catgttcacc atgacttacc cattcaagat
    36361 gcgggccaca ctccgggcag gccaccggct gggcgtggaa gcgacgatcg agcgggtcgc
    36421 ggtactcttt gtcacaggcc ggacatagcg gaaacgccgc catcacggta aacgggcggt
    36481 cgtaaggcat cgcgcgaata atggtgaaac gcgggccgca gtgggtacag ttgataaacg
    36541 gataacgata acgccgctcg cctggggtat tcatttcggc aaggcaagca gggcaagtgg
    36601 cggcatccgg gacaatttgc gtattcatgg tgccgccggt gctctggcgg atggtgaact
    36661 cggtgggcag ttgtgaccag ataaacggct cacgctcgac gctatcaata cgcgccagtg
    36721 gcggacagtg ctgatgcaat tgaacaagaa acgtttccgg atcttccagc agccggactt
    36781 ctacgccatc gccgtcatta catacatcgc cgtgaagatt taattgctgt gccagctgcc
    36841 agacaaacgg acgaaaaccg acgccctgca ctttgccacg aatacgcagt tggacaccgc
    36901 aagatgtgtt ttttgccatt gagttattcc cgccatcatg aattgcgtaa cccgccctgc
    36961 cggacacgac agcgtcgtat ccggcagtct caggtcggcg ataccgcccg ctccgtattc
    37021 tacgaatatt tccgggaatt cctttgatgc cagaacagtt ctgtaagatt tttagaacat
    37081 cagcgccgta cggcggcgtt tttctgcgct cagttgttca agtttattac gatcgacaca
    37141 aatcagcgca tgggtcgggc aagccgccat acacgccggg ccgtcttcac gatggttgca
    37201 caggtcgcat ttattggctt cggctttgtc agcccgtaca ttcagacccg cgccgctgtt
    37261 gcgaatcaca ggacgtacca ccacttccat cgcgccatac gggcaagcca ccacgcaggt
    37321 tttgcaacca atgcaacgtt cctgcatcac atgaacaaac cctttatcac ggctgatagc
    37381 accattcggg cagacgttag cgcacggtgc atcttcacac tgacggcaaa ctgtcgccgt
    37441 ggaaatgttc acacctttaa tgacatggat acgcggtaaa aatgtttccg gggtcagcga
    37501 tgcacagtcc tgattttcct gatgagaaac cacgcacgcc acttcacagg tacggcaacc
    37561 aatacattta ctcgcgtcag caatgatgaa acggttcatc aaattctcca gcaatgacag
    37621 ttaatgcgcc gatacattca caaatcatgc cagtttttaa tttgctgtta tttaaggaaa
    37681 ttaatttctg taatgcagga aaaaagatgt catcgacact agtgacgatg acatgtgatg
    37741 acaatgttta tcgcgaaagg gcaatgagtg agtcgcggcg gatcagtttt ccgctaaagg
    37801 ttttcggcgg tgagaaatcc ccgccatcga gcataaaaat cagccgtcca ataatttcct
    37861 gaatcatctc agtcaccgga atttttacgc tggagagcgc cggaacgata tagggggcaa
    37921 ttgcgatatc atcgaatccg ataactgaca cctgctctgg caccgctacg ccgcgctcgt
    37981 gcaacgcttt gatcgcacct atcgccatat cgtcgttact ggcaactaac gcgctaaatt
    38041 tagccccacg ttcgagcaac gtttctaccc cttcggcccc gctggcgggc gtccatttac
    38101 cgttagcgat aagtttttca ttgagcgcaa taccatgctg cgacagcgcg tctttatacc
    38161 cggcaagacg ttcaatgctg gtgggggaat ccatcgagcc ggtaaggaaa gcaatctcct
    38221 gatgcccggc gtttatcaac tctgccacgg cgttaaagct ggtctgttta tgatcgcacc
    38281 agacgctatg gctgctatta ttgcgcaggc ggcgattaag caccattatc ggctgactgt
    38341 gcgcgtcaat gatgtcatca atctcatcca cgcttaaaaa acgcgggtaa atcataatgg
    38401 cgtcgcagcg caaatccagc agatactgaa tcgcctggcg ctcttcttcg gcgctgtgtt
    38461 taccatctgc caatagcaac tgtcgccctt tctcttccgc cattcgcgcg gcatgaaaca
    38521 gtaattcact aaaataaatg ccgtgataaa gcgtgttggt cactaccagc cccagcgtct
    38581 gagtactctt cgccgacaga ttgcgggcca gcaaatttgg gcggtaactg ctctcttcta
    38641 ccgcctgaaa cacgcgatct ttagtctcct ggctgacgta gccattacct gaaagcacgc
    38701 gggaaacggt cgcttttgaa accccggcgc gcttcgccac ttccagcatc gtcgtcatca
    38761 ttttcatccc tttacacgca atcaacgcag tgtactgcac cgtttgccga ttgtccttgc
    38821 gcaatcggcg ggaaaaatat tcaggtgacc ggtttcacaa atataaaaaa tgaacaatta
    38881 actctcttgc ttatttagtg acaactattc atgattttgt gaaaccggtt tcttaattcc
    38941 gtttcagcat cggtattttt ccgtcacgtc gactgataac aactacatct accctactga
    39001 taacaggata aaatccgatg gccaaaaatt atgcggcgct ggcacgctcg gtgatagcgg
    39061 cactgggcgg cgttgataac atctcggcag tcacgcactg tatgacgcgt ttgcgctttg
    39121 ttatcaaaga tgaccagctt atcgacagcc cgacgttaaa aaccatcccc ggcgtgctcg
    39181 gcgtggtacg tagtgacaac cagtgtcagg taattatcgg caatacggtt tcgcaggcct
    39241 ttcaggaagt cgtcagcctg ctgccgggag atatgcagcc cgcacagccc gtgggtaaac
    39301 ccaaactcac gctgcgtcgc attggtgcgg ggatcctcga tgcgctgatc ggcaccatgt
    39361 caccgctgat cccggcgatt atcggcggat cgatggtcaa actgctggcg atgatcctcg
    39421 agatgagcgg cgtgctgaca aaaggatcgc cgaccttaac cattctgaat gtgattggtg
    39481 acggtgcttt cttcttcctg ccgctaatgg tcgcggcatc tgccgccatc aaatttaaaa
    39541 ccaatatgtc gctggcgatt gccatcgcgg gtgtgctggt acatccgagt tttattgaat
    39601 taatggcgaa agcggcccag ggtgaacatg tcgaatttgc cctgatcccg gtcaccgcgg
    39661 tgaaatacac ctacacggtg atcccggcgc tggtgatgac ctggtgcctg tcatatatcg
    39721 aacgctgggt ggatcgcatt acaccggcgg tgaccaaaaa cttcctcaag ccaatgctga
    39781 ttgtgttgat tgccgcaccg ctggcaatcc tgctgattgg cccgattggt atctggatcg
    39841 gtagcgccat ttcggcgctg gtttacacca ttcatggtta tctgggctgg ctttcagtcg
    39901 ccattatggg cgcgctgtgg cctctgctgg taatgaccgg gatgcaccgc gtctttacgc
    39961 caaccatcat tcagaccatt gccgaaaccg gcaaagaagg aatggtcatg ccatcagaga
    40021 tcggcgctaa cctgtcgctg ggcggttcat cactggcggt gccgtggaaa acgaaaaacc
    40081 cggaactgcg ccagacggcg ctggctgcgg cggcatcagc cattatggcg gggatttccg
    40141 aaccggcgtt atacggcgtg gcgatccgcc tgaaacgtcc gcttatcgcc agtcttatca
    40201 gcggttttat ttgcggcgcg gttgccggta tggcggggct tgccagccac tcaatggcag
    40261 cgccggggct atttaccagc gtgcaattct tcgatccggc gaatccaatg agcatcgtct
    40321 gggtgttcgc ggtcatggcg ctggcggtgg tgctgtcgtt tatcctcaca ctgttgctcg
    40381 gctttgagga tattcctgtt gaggaagcgg ctgcccaggc gcgaaagcat cagagcgtac
    40441 aaccgaccgt cgccaaagaa gtaagtctta attgaggatg aaaatgtcag tatttccaga
    40501 aggtttttta tggggcggcg cgcttgccgc caaccagtct gaaggtgcgt tccgtgaagg
    40561 cggaaaaggg ctgaccaccg tcgatatgat cccacacggc gagcatcgaa tggcggtgaa
    40621 actggggctg gaaaaacgtt ttcagttgcg cgatgacgag ttttatccca gccatgaggc
    40681 gacggatttt tatcatcgtt ataaagaaga tatcgccctg atggcagaga tgggattcaa
    40741 ggttttccgt acctcaattg cctggagccg tctctttccg caggacgatg aaatcacgcc
    40801 caatcagcag ggcattgctt tttatcgtcc tgtctttgaa gagtgtaaaa agtacggtat
    40861 cgaaccgctg gtcacgttgt gccacttcga tgtgccgatg catctggtca ccgaatatgg
    40921 ctcctggcgt aaccgcaagc tggtggagtt tttcagccgc tacgccagaa cctgctttga
    40981 agcatttgat ggtctggtga aatactggct aaccttcaat gaaatcaaca ttatgttgca
    41041 tagcccgttc tccggcgcgg gtctggtgtt tgaagaaggt gaaaatcagg atcaggtgaa
    41101 atatcaggcc gcgcatcacc agctggttgc cagtgcgcta gccaccaaaa tcgcccatga
    41161 ggttaacccg caaaatcagg tgggctgtat gctggcgggc ggtaacttct acccttacag
    41221 ctgcaaaccg gaagatgtct gggcggcgct ggagaaagat cgggaaaacc tgttttttat
    41281 cgatgtgcag gcgcgcggcg cgtatccggc ttactctgcc cgcgtatttc gcgaaaaagg
    41341 ggtaaccata gataaagcgc tgggcgatga tgagatcctg aaaaacaccg tcgattttgt
    41401 ctctttcagc tattacacct cgcgctgcgc ctcggcggag atgaacgcca acaacagcag
    41461 tgcggcgaac gtggtgaaat cgctgcgtaa cccgtatctg caagtgagcg actggggctg
    41521 gggaattgat ccactcggtc tgcgtatcac catgaatatg atgtacgatc gttatcagaa
    41581 gccgctgttt ctggtggaaa acggcctcgg cgcaaaagat gaacttgctg ccaatggcga
    41641 gattaacgat gactatcgca tcagctattt acgcgaacat atccgcgcaa tgggcgaagc
    41701 gattgcagat ggcattccgc tgatgggcta caccacatgg ggctgtattg atttagtttc
    41761 cgcctctacg ggtgaaatga gcaaacgcta cggttttgtc tacgtagacc gtgacgacgc
    41821 aggcaacggc acgctgacgc gcacacgtaa gaaatcgttc tggtggtata aaaaagtgat
    41881 tgccagtaat ggggaagatt tagagtaagt aacagtgccg gatgcggcgt gaacgcctta
    41941 tccggcctac atagttcgtg cgacatgtag gcccgataag cgcagcgcat cgggcaattt
    42001 agcatttggc attagtcaaa aatcaaagaa accattccgg cttaacgcca aatcgggctg
    42061 aaagtgcctt aatgtgggat atcgtcaggg atcgttgtcc agacaaaatc tgactgacca
    42121 gggatttaga gccgatttcg ttcttcaaat ccgcataggt cagattatgt tgatcaatca
    42181 gcgtgcgaag taacgccacg ccaacaggca ttgccgcaac ggctttgtcg aactctgcaa
    42241 actcttggtt gttgttttca tattctgcaa tgcggctcgc cagaaagtcg ataagtggat
    42301 tggtgtcatc ttcttcaatc aaatattcga ccagttccag tgcttcacga taatcatctt
    42361 ctgagtcact acctcccaga aaggggactg cattgaccag ttctcttgtt gctttaacag
    42421 cgtgtgcagc attagcagtc attctttgtt ctctctgtag taacgtgtca gtttgtcata
    42481 ctcggcgtga ttagttatgt gcttcacaaa gaaccgctta ttaacaaagt ttatgtaggc
    42541 aatcacccct aggttgttcc cacctacatc cagaacgtac catttgttgc gatatttaaa
    42601 attgtcgagg ttgggaaacg ccgttcgcat ctcttctggc gtagaaaaat ccgtttcctt
    42661 aataacccga tacaatgcct gaagagccag agcgtcgtta ggatatttac gcgcagactc
    42721 ttcaaatggc gctttagaaa tgatgtgcat ttttaagttt taacttcctt atagtaagta
    42781 aacagccaca tctgcgagtt ctatccttat gtgttcggaa actatagctc atcaaatcca
    42841 agttcacaat atgtaaacac ttgtgcgcga tataattacc cccaaaatcc tcatagccac
    42901 tctgcaaaaa gtcagttctg gttcacccct caagcacatc cccctttatc aagcaacgct
    42961 gcgtctgcgc cagatcttcc gtttgcgggt cgcgcccggc gatgggtttg aggacgagtt
    43021 tacggttggg ggtataagta aaacgcgtga gcgcgggata gcttaacttg tcaaaccagc
    43081 cgtgaacacg ctgccatttg gcgtctttta cctgatacag actggttatc cagtaaccag
    43141 aattaaactg catatccagc aattgcgtgt gaccattgcc ttgcaaatcc agcaaatcat
    43201 ctacgccagt tttgcttgcg gtgtagaatc ctctgggttc aaagacacag ggacggcctt
    43261 cgcgcgtgaa cgtcataaga attaaatgtg cataaggagc cagaccatta ccggtacttg
    43321 gtaaccagat aaccagatcc tgaatgccgt tacgatcaag atcggcgcga tagagttgcc
    43381 ctttgcccgc tatgtttgcc agcataccca caggcacagt ccaggattta ccggcgcggt
    43441 ctttagcggt taattgccag taatcttctt catttggcat cttctcaagc gtcacgccag
    43501 caacgcccac tttcctaaaa tgccaggttt gctcggcatc ataaggcatg gtcaggagag
    43561 gtttcagcgc ccgcttttgc gcgtattcgc tgtcactcat tggcagatat tcggtaaagc
    43621 tggtggcctg tgcaggcagc actaagctca ataaaaaagc gcacatccct gttctgatgt
    43681 tcaattccct gaattccctg aattccctga tggagaatta ttcttcttcc accgctaact
    43741 gcgcaaatcc accatttcct tcccagcctt ccagtcgttg ataaacggtt tctaccgcat
    43801 ctttaatcgg ctgggtcatc gggtagtaaa agccgacgat atccggctga atgccgagga
    43861 aaatcacttc accaatgtct tcttttaact ggtcgataag gtaattcaac ggcatgttat
    43921 gggtagtcat cataaacatc tcggcgatat catccgggtc gatgatgcgg atttcgccgg
    43981 ggtttagccc catatccgtg gcgtcgacaa tcagcagtcg tgtcgggcgc agttcacgga
    44041 tggcgacgat gtcgttttcc ggtgcactac cgccgtcaat caccacccag ttacctttcg
    44101 gcgcggcggc gcacttttcc gccagcagcg ggccagcgcc atcatcgccc atcatgctat
    44161 tgccaacaca gagtaaaacg tcagtcacgc agtctcctca ccatcaaata gatggcgttt
    44221 tcctgatgaa tatcatgcag catacttaac agcattgtac tccattcctg ctgctgcgaa
    44281 gtttgcgctg cccgggcttt atcgaatgcg ttggccagca tcggcacatg gttaatgtcg
    44341 atgacgatct cgccatattt cggtacgcct tccattttgt ggcgggcttc gctgcccgcc
    44401 tccagagtgg caatccatgc cagatattca tcccatgggc aggtgagcgc cgcttccagg
    44461 caatcgataa ccccaaggtg gtgaccaatc gccaggctgt aatagaccac ctgctgcgcc
    44521 tcggcgggcg tggcatcgtt ctcatcaata aatttacggc tcagttgact gaacaccacc
    44581 ttttcactca tcggatacgc gcctcttcaa caacatgatt cagatggctg acaatctcgt
    44641 tcagacgcgg atcgttttcc gcttccagcc agcgtgcaac ctgttcttcg ccctgcccta
    44701 actgtgtaag gtaatcatcg gcaatctgac gaccgtaacg ataacccgcc aggcgacgtg
    44761 cttcgcgatc cactttcacg cgcagcggct gcaccatatc gccatgcagg atctccgccg
    44821 gttgttcatc cagttcaccc ggcccacggg cgtgaatttt ctgctccagc aggccgagcg
    44881 ccattgcaaa gccgtacagc gtggcggcag gcgttggcgg gcagctaggg atataaacat
    44941 ccacagggac aattttatcc gtaccgcccc acacgcagta gagatcgtgt aagatcccgc
    45001 cgctgttacc gcaggcaccg tatgagatac agattttcgg gtccggcgcg gactgccacg
    45061 cacgcagcgc aggggatcgc attgcacggg tgaccgcgcc ggtaaacagt aaaatatccg
    45121 catgacgcgg tgaaggaacg actttaatgc cgaagcgttc tgcatcgaac agcggagaaa
    45181 gcgtggcgaa aatttcgatt tcgcagccgt tgcagccgcc gcagtccacg cggtaaacat
    45241 aggcagaacg tttgattttt ttcagtaacg acgccttcat gctggcgatg gattcatcca
    45301 ccgtcatcgg gaccggaatg ccgttggcgt cacgggggcc taataaattg ctcatcagat
    45361 ggcctctttc atatggcgag tcagttcaat acggtcggac ggcaccaggc atttctggcg
    45421 cttacattcc gggcaagtct caaagctttc gcggtggttt tccgcgcggc tgtcaccatt
    45481 gtgcttaagc agcgcaatgg cgtagtcgat ctctttctgt acggcgaaag gacgattgca
    45541 gacgcggcag ttgcacagcg cgaagcggga ctgttgcagg aagtcttctt tcttccacac
    45601 cgccagttcg tactcttgcg acagtttgat cgccgccgtc gggcaaactt cttcgcagcg
    45661 tccacagaag atgcagcgcc cgagattaaa ctcccacgcc agctctcctg tggcgaggtc
    45721 agtttcaacc gttaaggcgt ttgacgggca ggcattgacg caagccgcgc agccgatgca
    45781 ctgctgcggg ttctgctctg gcttaccacg gaagttttta tcaaccgcaa tcggctccag
    45841 cggataagac gaggtcgccg tgccggtttt gatgactttt ttgataaagg taaacatggc
    45901 gattccttat ttcagcggcg agtttttacg ctcaatgctg taacgctcga gttctttgta
    45961 cggcaccact ttgctcttct tcttacgcac atcgactacg gtcatgcggt cggtacagga
    46021 gtagcaaggg tccaggctac cgataatcag cggcgcatcg gaaacggtat tgccacgcag
    46081 catgtagcgc agggtcggcc agttcgcgta ggtcgcggca cggcagcgcc agcggtacag
    46141 cttctggttg tcgccggtca tgctccagtg gatatcgtcg ccgcgcggcg cttcggcaaa
    46201 gcccagcgcg aaacggtgcg gaatgtaggt aaagccttcc accatcagcg gaccgcccgc
    46261 aggttatcca gaccgtagtc gatcatattc agcgcggtat agacctcgtt gattcgcact
    46321 ttcagacggg aaataacgtc gcagccctgc tcgctgtgga cttccattgg cagcaggcca
    46381 taaccgacaa acgggtgatc ggcgcgagta tcgcgggcga gaccgctggc gcgaaccatc
    46441 gggccaacgt tactgaaatc gcaggcgatt tccgggtcca gacgaccaat gccgacggtg
    46501 cgttgttcca tgttcggcgt actcagcagc acatccacca gctcctgcac ttcacgacgc
    46561 atttgttgcg ccagctggcg ggtctggatc atgtcatctt tcagcagatc gtgacgaatc
    46621 ccgccgatca ggttcaggcc gtaggtttta cgcgcaccgg taaggatctc tgccattttc
    46681 atggaggttt cacgcacgcg gaagaactgc ataaagccgg agtcgaagcc ggtaaagtga
    46741 caggcgaggc cgaggttgag cagatgcgag tgcaggcgtt ccacctccag cagaatggcg
    46801 cggatcatct gcgcgcgttc tggcacctga atacccatcg cgttttccac cgacgtggtg
    46861 taggcggtgc tgtgggcaaa gccgcagatc ccgcacacac ggtcagagag gaaggtcact
    46921 tcgttataac ccatgcgggt ttccgccagt ttttccatac cgcgatggac gtagaacaga
    46981 cggtagtcgg cgtcgataat gttttcgcca tcgacgaaca gacggaagtg acccggttcg
    47041 tcagaagtga cgtgcagcgg accaatcggc acgacgttgt ttttcttgtc gcccagttcg
    47101 ttgatgaact cgtaggtttc agcatcggta gtcggtgccg gacgctgacg ataatccatg
    47161 ctgtctttac gcagcggata aagttcatcc ggccagtcat ccggcagacc aaccggaatc
    47221 aaaccgtaca tatcgcgcac ttcgcgctcg ccccacaccg ccgccggaac gcgcggcgtc
    47281 acggacggat actccggttt gttggcgtca acttcgacgc gaaccgttac ccaacacttg
    47341 gtgcccttct ccatcgacag cacgtaataa acggcgtaat gaccattcag tttgcgttcg
    47401 tcgttaccaa acagcaccga cagccagcca ccctgcttgt agtagagaaa ctccaccact
    47461 tccggcagat agttcacctt tacggtgaca gtcagctgat ctttggtctg ccaggcgtgg
    47521 tccagcacga cgcctggaaa tgcctcattc agcgcggcga gataatgttg acctaatttt
    47581 tcttcagaca tgctcaaact ctctttaatc acgccgccag caaggagacg aacgctaaaa
    47641 atgcaaagcc aaacccagcc caggtaatgc gcggagtaat atcaagacgc agacgggcca
    47701 tactgttttc gaacagcgcg ataaccagga cgccgaccac cagtttgacg atggcaatca
    47761 ccagcgccag cagcagtcca ccgacggtga aggtttccat ttgtccccac ggaataaaca
    47821 ccccgacgaa catctgcaac accaccagct gtttcaggct gataccccat ttcatgacgc
    47881 caaagccgct accgctgtat tcggagagcg gaccttcctg caactcctgc tcggcttccg
    47941 ccaggtcgaa cggcagtttg cccatttcga taaaagtggc gaacgcacag gcgcagagcg
    48001 ccagcaccag cgggatgctt tggctcagcg gccagtgata aacggtgtcg gtaatgttgc
    48061 tgatgttggt ggaaccggca acctgtgcgg caacccacag accaagcagc aacatcggtt
    48121 cgaccagcac gccaagcatc gcttcacggc tcgcaccgat agcggtaaac gggctaccgg
    48181 tatccagacc ggaaatggca aagaagaaac gcgcgatggc gaagagatac agtaaggtga
    48241 tcaaatcgcc cagttgcggc agcggagaac cgatagtcag catgacgccc accatcacat
    48301 acggcgtcag gcggaacatc cagccagagg catccgggcc aacgctctgg cggcccagca
    48361 gtttgataat gtcgcgatac tcctgcaaca cgcccggccc gcgacggtta tgcagacggg
    48421 cgcgcgccac gcgggtaata ccggagagca gcggcgcaac ggcaaataac accagcgcct
    48481 gaattaacgg atataaaaca ctcattctca ggctcctcgt gaaacaataa tcaccaccag
    48541 taccgccagt tcaaccagcg ccatccggcg gaacagcaac gcactacctt cgcactgcca
    48601 gcccggcacc agcgacaccg gattcagcca tttgcgcagt ttcagcactg gcgcaaacgc
    48661 ctgtttaacc ggcatggcaa aaccgtgagc ggtaatcacc attgattttt catggtcgta
    48721 gccgcacacc caggccgcac cgcgggaacg tgacggcagg cggtcgcctt tgcaaatcgc
    48781 cataatgatg aatggcagca gcgggcaggc aatcagcagc aatgtgatca ttggttgaga
    48841 aacggtggtg ttagcaggct ccagaggcag aggtacagca gcagagagca tcggcagcag
    48901 ccacggcgca gcaacaccgc caattacgca gcaaatcgcc agtgccacta cgcttacgct
    48961 catcaggagc ggcgcacagg tggcgttttc ggcttctttg gtacgcggcg cgccgaggaa
    49021 cgtgacgcca tagactttcg ccatacacat caccgccagc gcaccggtaa ttgccagacc
    49081 cacagcgagc agcggcccca gcagacgggc aacaaacgcg ccgctattgc tcagtttgaa
    49141 gaaggattga tagataaccc attccccggc aaacccgttc agtggcggca gcgcagccat
    49201 tgccatcaac ccgactaaca tggcgatgga gataaccggc attttcttgc caataccacc
    49261 gagtttttcg atatcgcgat gaccggtacg gaaccagacg ctccccgccc cgaggaacag
    49321 tacgcttttg aacaggctat ggttaagcag atggtacaga ccaccgacca ggccaagagc
    49381 aatcagcgcc ggttgttcga gcgcgatacc cgttacgcca gcgcccagcc ccagccctag
    49441 ccctagccct agccctagcc ctagcaggat aatgccgata ttttccaggg tgtggtaagc
    49501 cagcaggcgc tggatgttgt gttccatcaa cgcatacaga ccaccgacga acgcggtgat
    49561 catgccgagc accagcagcg cgatccccca ccacagcggt gcattaccgc ccagcagtga
    49621 cagggttaac atgcccagca ggccaatttt catgactacc gtagaaaaca acgcggcagc
    49681 tggcgcagag gcgttagcat gtgcctgcgg cacccagccg tgcagcggaa taatcccagc
    49741 cagcaggcca aagccaatca caccgagcag ccagatatcg gaaccgagca gccagatatc
    49801 ggaaccgagc ggcagctgtt gcatacgcat atccagcagg cgcagatcca gcgtgccata
    49861 acgctgccac agcagccagc aagcaatcac cagcagcaga gtgccaagac gccccagcgc
    49921 aaaccacagt ttgccctctt tgctgttgct ggtgaggaac accgcacaca aggccatgac
    49981 ttcggccatt accacgaaca tgccgaggtt gctggcgatg acggcgcaga cggcggcagc
    50041 catcaacata ttgatctgca agccgttgca cttcacctgc gcgtggcgat gccagtcaat
    50101 gttatagagg ctgacaaaca gaccgcaaag gccgagggta atcagccaaa tcgcgttaag
    50161 cggggagatt tgcacatcgt agcttaccag cgacatcgca ccgctcacgc caaccgagcc
    50221 agtcagtaca gtgaagcccg cggctgccgt atacagacta ccaaccgcgc cgccaattcc
    50281 ggctatccag ccacttagcg ctttttgaaa agaaaagaga aatgccagaa cagcggcggc
    50341 gacaaaccat gccacgccgc tattgatcag ggaaattgcg ctcatttagc ctctccactt
    50401 tgagcctgct gaaacaaggt gagatcgcca aagtccgtgt taaaagtcag ctcacgctta
    50461 cgtttgctga cgcgggcgat atcggtgtta tccaccagat gcagggcttt agtcgggcac
    50521 atccgcacac aggccggacc ttgttcatca aagctacaaa ggtcacattt gacggcgatc
    50581 gcgcgaatac ctgacaccca gtcaagcaat gtgctgacac gcgccggagc aggcggtgcc
    50641 ggtggcgctt tcggggtatt ggcgtttgcc ggaatatcca gcggacggct gccggaaaat
    50701 tcaattgcgc caaacgggca ggcgatgccg cacagtttgc agcttacgca cagactttca
    50761 ttcaactgca cggccccatc aacgcgggtg atggcgttaa ccgggcagac caccgcgcag
    50821 ggtgcatctt cacagtggtg acagagcagc ggcgcagatt ctttttcatt gagcatcact
    50881 ctcaggcgcg gcattgattg caggccgtgc tggcgatgcg tctctgaaca ggcggcctca
    50941 caggtgtggc agccgataca gagcgtggag tcggcaatta caaaacgatt caccaggcat
    51001 tcctcaggtg attgtcattt ttgacgaaaa catgccgatg aaatgtcatt ttcgacactc
    51061 atcgacacgc ccatccccaa acaggcgtaa cgcctgcaaa acgggcaaag cctcagctca
    51121 tgctgccggg ctttgtccct ttaccagttg gcttaaattc accggcacat tgttttcaat
    51181 ggcggtgtat aagctgtcgt aaacgccagc gattttttcg agatagtgtt ccagaacctg
    51241 ttcgcgatcg cggatgttga ccttgccatc aatcatgccg tcaatgctca gttgcgcctg
    51301 cgcaatccgc tgtttgtctt cgccatcttt tgtttcgacg taatactcga tggtgtggct
    51361 gttaaagcca tccgccaggg tgacgtaaat acgaaaatgt tcaaaaagga cgaaacagag
    51421 ttctttgtcc ggcgcgcagc tcatggcgtg atgcaggcgc gctttcgata acgtgatccc
    51481 ctgaaccagc gaattgcagt agatgtgcca ctggtcctgt aggcgacgat gccgctgtgc
    51541 gatgtaatcg gctttctcgc ttatttccca aatagtcatt gtcaggttac tcgtttaaca
    51601 gagatgccag ctttaagcat tttttgtacc aacttttaat ttattgttat taaagagatt
    51661 tttaagctaa agatgaattt cgtcgccgtg tcgacgtgtc atttcgacat catcgacatt
    51721 attcaccgca gggataatca acactggcgc aattattgct tgtagctggc aatagttaat
    51781 gggaggcgat atgcacgaaa taaccctctg ccaacgggca ctggaattga tcgaacagca
    51841 ggccgcaaaa cacggcgcaa aacgcgtaac tggggtctgg ctcaaaattg gcgcattttc
    51901 ttgtgtcgaa accagctctc ttgccttttg ttttgatctg gtttgccgcg gcagcgtggc
    51961 ggaaggttgt aaactgcacc tcgaagaaca agaggccgaa tgctggtgtg aaacatgcca
    52021 acagtatgtg acgctactga cccagcgcgt ccgccgctgt ccacagtgtc atggtgacat
    52081 gctacagatt gtggcagacg acggtttaca gattcggcgg atagaaatag accaggagtg
    52141 agcgatgtgt acaacatgcg gttgcggtga aggcaacctg tatatcgagg gtgatgaaca
    52201 taaccctcat tccgcgtttc gtagcgcgcc atttgccccg gcggcacgcc cgcagatgaa
    52261 aatcaccggc attaaagcgc ctgaatttac ccccagccag actgaagaag gcgacctgca
    52321 ttacggtcat ggcgaagcgg gcactcacac accgggcatg agccagcgtc ggatgctgga
    52381 agtcgaaatt gacgtgctgg acaaaaacaa ccgtctggct gaacgcaacc gcgcgcgctt
    52441 tgctgcccgc aagcaactgg tgctcaacct ggtttccagc cctggttccg gtaaaaccac
    52501 cctgctgacg gaaaccttaa tgcgcctgaa agacagcgtt ccgtgcgcgg ttattgaagg
    52561 cgaccagcaa accgtgaacg atgccgcacg cattcgcgct accggtacac cagcgattca
    52621 ggtgaacacc ggtaaaggct gccatcttga cgcacagatg attgccgacg ccgcaccgcg
    52681 tctgccactg gacgataacg gtattctgtt tatcgaaaac gttggcaacc tcgtatgccc
    52741 ggccagcttc gatctcggtg aaaaacacaa agtagcggtg ctttccgtta ccgaaggtga
    52801 agacaaaccg ctgaaatatc cgcatatgtt tgccgccgcc tcgctgatgc tgctcaacaa
    52861 agttgacctg ttgccgtatc tcaactttga cgttgagaag tgcatcgcct gcgcccgcga
    52921 agtcaatcca gaaattgaaa tcatccttat ttccgccacc agcggcgaag ggatggacca
    52981 gtggctgaac tggctggaga cacagcgatg tgcataggcg ttcccggcca gatccgcacc
    53041 attgacggca accaggcgaa agtcgacgtc tgcggcattc agcgcgatgt cgatttaacg
    53101 ttagtcggca gctgcgatga aaacggtcag ccgcgcgtgg gccagtgggt actggttcac
    53161 gttggctttg ccatgagcgt aattaatgaa gccgaagcac gcgacactct cgacgcctta
    53221 caaaacatgt ttgacgttga gccggatgtc ggtgcgctgt tgtatggcga ggaaaaataa
    53281 tgcgttttgt tgatgaatat cgcgcgccgg aacaggtgat gcagttaatt gagcatctgc
    53341 gcgaacgtgc ttcacatctc tcttacaccg ccgaacgccc tctgcggatt atggaagtgt
    53401 gtggcggtca tacccacgcc atttttaaat tcggcctcga ccagttactg cctgaaaacg
    53461 ttgagtttat ccacggtccg ggttgcccgg tgtgcgtact gccgatgggt agaatcgaca
    53521 cctgcgtgga gattgccagc catccggaag tcatcttctg tacctttggc gacgccatgc
    53581 gcgtaccagg gaaacaggga tcgctgttgc aggcaaaagc acgcggtgcc gatgtgcgca
    53641 tcgtttactc gccgatggat gcgttgaaac tggcgcagga gaatccaacc cgcaaagtgg
    53701 tgttcttcgg cttaggtttt gaaaccacca tgccgaccac cgccatcact ctgcaacagg
    53761 cgaaagcgcg tgatgtgcag aatttttact tcttctgcca gcacattacg cttatcccga
    53821 cgctgcgcag tttgctggaa cagccggata acggtatcga cgcgttcctc gcgccgggcc
    53881 acgttagtat ggttatcggc actgatgcct ataattttat cgccagcgat tttcatcgtc
    53941 cgctggtggt cgctggtttc gaaccccttg atctactgca aggcgtggtc atgctggtgg
    54001 agcagaaaat agcggcccac agcaaggtag agaatcagta tcgtcgggtg gtaccggatg
    54061 ccggtaacct gctggcgcaa caggcgattg ccgatgtgtt ctgtgtcaac ggcgacagcg
    54121 aatggcgcgg cttaggcgtg attgaatctt ctggcgtgca cctgacgccg gattatcaac
    54181 gattcgatgc cgaagcacat ttccgcccgg caccgcagca ggtctgcgat gacccgcgcg
    54241 cgcgttgtgg tgaggtatta acgggcaaat gtaagccgca tcaatgcccg ctgtttggta
    54301 acacctgtaa tcctcaaacc gcatttggtg cactgatggt ctcctccgaa ggagcgtgcg
    54361 ccgcgtggta tcagtatcgt cagcaggaga gtgaagcgtg aataatatcc aactcgccca
    54421 cggcagcggc ggccaggcga tgcagcaatt aatcaacagc ctgtttatgg aagcctttgc
    54481 caacccgtgg ctggcagagc aggaagatca ggcacgtctt gatctggcgc agctggtagc
    54541 ggaaggcgac tgtctggcgt tctccaccga cagttacgtc attgacccgc tgttctttcc
    54601 tggcggtaat atcggcaagc tggcgatttg cggcaccgcg aatgacgttg cggtcagtgg
    54661 cgctattccg cgctatctct cctgtggctt tatcctcgaa gaaggattgc cgatggagac
    54721 actgaaagcc gcagtgacca gcatggcaga aaccgcccgc acggcaggca ttgccatcgt
    54781 tactggcgat actaaagtgg tgcagcgcgg cgcggcagat aaactgttta tcaacaccgc
    54841 tggcatgggc gcaattccgg cgaatattca ctggggcgca cagacgctaa ccgcaggcga
    54901 tgtattgctg gttagcggta cactcggcga ccacggggcg actatcctta acctgcgtga
    54961 gcagctgggg ctggatggcg aactggtcag cgactgcgcg gtgctgacgc cgcttattca
    55021 gacgctgcgt gacattcccg gcgtgaaagc gctgcgtgat gccacccgtg gtggtgtaaa
    55081 cgcggtggtt catgagttcg cggcagcctg cggttgtggt attgaaattt ctgaatcagc
    55141 gctgccggtt aaacctgccg tgcgcggcgt ttgcgaattg ctgggactgg acgccctgaa
    55201 ctttgccaac gaaggtaaac tggtgatcgc cgttgaacgc aacgcggcag agcaagtgct
    55261 ggcagcgtta cattcccatc cactggggaa agacgcggcg ctgattggtg aagtggtgga
    55321 acgtaaaggt gttcgtcttg ccggtctgta tggcgtgaaa cgaaccctcg atttaccaca
    55381 cgccgaaccg cttccgcgta tatgctaata aaattctaaa tctcctatag ttagtcaatg
    55441 accttttgca ccgctttgcg gtgctttcct ggaagaacaa aatgtcatat acaccgatga
    55501 gtgatctcgg acagcaaggg ttgttcgaca tcactcggac actattgcag cagcccgatc
    55561 tggcctcgct gtgtgaggct ctttcgcaac tggtaaagcg ttctgcgctc gccgacaacg
    55621 cggctattgt gttgtggcaa gcgcagactc aacgtgcgtc ttattacgcg tcgcgtgaaa
    55681 aagacacccc cattaaatat gaagacgaaa ctgttctggc acacggtccg gtacgcagca
    55741 ttttgtcgcg ccctgatacg ctgcattgca gttacgaaga attttgtgaa acctggccgc
    55801 agctggccgc aggtgggcta taccccaaat ttggtcacta ttgcctgatg ccactggcgg
    55861 cgggagggca tatttttggt ggctgtgaat ttattcgtta tgacgatcgt ccctggagcg
    55921 aaaaagagtt caatcgtctg caaacattta cgcagatcgt ttctgtcgtc accgaacaaa
    55981 tccagagccg cgtcgtcaac aatgtcgact atgagttgtt atgccgggag cgcgataact
    56041 tccgcatcct ggttgccatt accaacgcgg tgctttcccg cctggatatg gacgaactgg
    56101 tcagcgaagt cgccaaagaa atccattact atttcgacat tgacgatatc agcattgtct
    56161 tacgcagcca ccgtaaaaac aaactcaaca tctactccac tcactatctt gataaacaac
    56221 atcccgccca cgaacagagc gaagtcgatg aagccggaac cctcaccgaa cgcgtgttca
    56281 aaagtaaaga gatgctgctg atcaatctct acgagcggga cgatttagcc ccctatgaac
    56341 gcatgttgtt cgacacctgg ggcaaccaga ttcaaacctt gtgcctgtta ccgctgatgt
    56401 ctggcgacac catgctgggc gtgctgaaac tggcgcaatg cgaagagaaa gtgtttacca
    56461 ctaccaatct gaatttgctg cgccagattg ccgaacgtgt ggcaatcgct gtcgataacg
    56521 ccctcgccta tcaggaaatc catcgtctga aagaacggct ggttgatgaa aacctcgccc
    56581 tgaccgagca gctcaacaat gttgatagtg aatttggcga gattattggc cgcagcgaag
    56641 ccatgtacag cgtgcttaaa caagttgaaa tggtggcgca aagtgacagt accgtgctga
    56701 tcctcggtga aactggcacg ggtaaagagc tgattgcccg tgcgatccat aatctcagtg
    56761 ggcgtaataa tcgccgcatg gtcaaaatga actgcgcggc gatgcctgcc ggattgctgg
    56821 aaagcgatct gtttggtcat gagcgtgggg cttttaccgg tgccagcgcc cagcgcattg
    56881 gtcgttttga actggcggat aaaagctctc tgttcctcga cgaagtgggc gatatgccac
    56941 tggagttaca gccgaagttg ctgcgtgtat tgcaggaaca ggagtttgaa cgtctcggca
    57001 gcaacaaaat cattcagacg gacgtacgtt taattgccgc gactaaccgc gatctgaaaa
    57061 aaatggtcgc cgaccgagag ttccgtagcg atctctatta ccgcctgaac gtattcccga
    57121 ttcacctgcc gccactacgc gagcgtccgg aagatattcc gctgctggcg aaagccttta
    57181 ccttcaaaat tgcccgtcgt ctggggcgca atatcgacag cattcctgcc gagacgctgc
    57241 gcaccttgag caacatggag tggccgggta acgtacgcga actggaaaac gtcattgagc
    57301 gcgcagtatt gctaacacgc ggcaacgtgc tgcagctgtc attgccagat attgctttac
    57361 cggagcctga aacgccgcct gccgcaacgg ttgtcgccca ggagggcgaa gatgaatatc
    57421 agttgattgt gcgcgtgctg aaagaaacca acagcgtggt tgccgggcct aaaggcgctg
    57481 cgcaacgtct ggggctgaaa cgcacgaccc tgctgtcacg gatgaagcgg ctgggaattg
    57541 ataaatcggc attgatttaa ctgcaaattg ccggacagat ctgcctgtcc ggcatactat
    57601 tcacgaggtt ttttcggacg atatttttcc ggcagttctg gcaccggacg cttgtcatcg
    57661 atgagatgac gcacggttaa gatcggatga cgccacagca ttctcggccc ggcccaacgc
    57721 ataatctgtt tcatctcttc acgctttgca ggctggtaac agtgcaccgg acactgctta
    57781 caggctggtt tctcttcgcc gaacacacat ttatccagcc gcttttgcgc gtaaacaaac
    57841 aacgcctcgt aatgctccgg ctccgctgac gcctgcgggc atttcgcttg ataaagatcg
    57901 atcatttttt taatcgtcag tttttcacga gagatacgct tgccggacat gctgcctcca
    57961 cctcattaag atgcatttat attacatctt aatcttaaag ggcactatga ctctaaagaa
    58021 gaagggttcg ccaatcgata caattttgcg tacttgcttc ataaacatca cgcaaaagct
    58081 gcaaaacagc atctttcccg gaaccagcat caagaactcg ccgttcgctt cttcccctga
    58141 aatgattaac tccggtatca tgtgcgcctt atgtgattac aacgaaaata aaaaccatca
    58201 caccccattt aatatcaggg aaccggacat aaccccatga gtgcaataga aaatttcgac
    58261 gcccatacgc ccatgatgca gcagtatctc aagctgaaag cccagcatcc cgagatcctg
    58321 ctgttttacc ggatgggtga tttttatgaa ctgttttatg acgacgcaaa acgcgcgtcg
    58381 caactgctgg atatttcact gaccaaacgc ggtgcttcgg cgggagagcc gatcccgatg
    58441 gcggggattc cctaccatgc ggtggaaaac tatctcgcca aactggtgaa tcagggagag
    58501 tccgttgcca tctgcgaaca aattggcgat ccggcgacca gcaaaggtcc ggttgagcgc
    58561 aaagttgtgc gtatcgttac gccaggcacc atcagcgatg aagccctgtt acaggagcgt
    58621 caggacaacc tgctggcggc tatctggcag gacagcaaag gtttcggcta cgcgacgctg
    58681 gatatcagct ccgggcgttt tcgcctgagc gaaccggccg accgcgaaac gatggcggca
    58741 gagctgcaac gcactaatcc ggcggaactg ctgtatgcag aagatttcgc cgagatgtcg
    58801 ctgattgaag gccgtcgcgg cctgcgccgt cgcccgctgt gggagtttga aatcgacacc
    58861 gcgcgccagc agttgaatct gcaatttggc acccgcgatc tggtcggttt tggcgtcgag
    58921 aacgcgccgc gcggactttg tgctgccggt tgtctgttgc agtatgcgaa agatacccaa
    58981 cgcacgaccc tgccgcatat tcgttctatc accatggaac gtgagcagga cagcatcatt
    59041 atggatgccg cgacacgtcg taacctggaa attactcaga acctggcggg cggtgcggaa
    59101 aatacgctgg cttctgtgct cgactgcacc gtcacgccga tgggtagtcg tatgctgaaa
    59161 cgctggctgc atatgccagt gcgcgatact cgcgtgttgc ttgagcgcca gcaaactatt
    59221 ggcgcattgc aggatttcac cgccgagttg cagccggtac tgcgtcaggt cggcgacctg
    59281 gaacgtattc tggcacgtct ggctttacga actgctcgcc cacgcgatct ggcccgtatg
    59341 cgccacgctt tccagcaact gccggagctg cgtgcgcagt tagaaactgt cgatagtgca
    59401 ccggtacagg cgctacgtga gaagatggga gagtttgccg agctgcgcga tctgctggag
    59461 cgagcaatca tcgacacacc gccggtgctg gtacgcgacg gtggtgttat cgcatcgggc
    59521 tataacgaag agctggatga gtggcgcgcg ctggctgacg gcgcgaccga ttatctggag
    59581 cgtctggaag tccgcgagcg tgaacgtacc ggcctggaca cgctaaaagt tggctttaat
    59641 gcggtgcacg gctactacat tcaaatcagc cgtgggcaaa gccatctggc acccatcaac
    59701 tacatgcgtc gccagacgct gaaaaacgcc gagcgctaca tcattccaga gctaaaagag
    59761 tacgaagata aagttctcac ctcaaaaggc aaagcactgg cactggaaaa acagctttat
    59821 gaagagctgt tcgacctgct gttgccgcat ctggaagcgt tgcaacagag cgcgagcgcg
    59881 ctggcggaac tcgacgtgct ggttaacctg gcggaacggg cctataccct gaactacacc
    59941 tgcccgacct tcattgataa accgggcatt cgcattaccg aaggtcgcca tccggtagtt
    60001 gaacaagtac tgaatgagcc atttatcgct aacccgctga atctgtcgcc gcagcgccgt
    60061 atgttgatta ttaccggccc gaacatgggc ggtaaaagta cctatatgcg ccagaccgca
    60121 ctgattgcgc tgatggccta catcggcagc tatgtaccgg cacaaaaagt cgagattgga
    60181 cctatcgatc gcatctttac ccgcgtaggc gcggcagatg acctggcttc cgggcgctca
    60241 acctttatgg tggagatgac tgaaaccgcc aatattttac ataacgccac cgaatacagt
    60301 ctggtgttaa tggatgagat cgggcgtgga acgtccacct acgatggtct gtcgctggcg
    60361 tgggcgtgcg cagaaaatct ggcgaataag attaaggcat tgacgctgtt tgccacccac
    60421 tatttcgagc tgacccagct accggagaaa atggaaggcg tcgccaacgt acatctcgat
    60481 gcgctggagc acggcgacac tatcgccttt atgcacagcg tgcaggacgg cgcggcgagc
    60541 aaaagctacg gtctggcggt tgctgctctg gcgggcgtac cgaaagaggt tattaagcgc
    60601 gcacggcaaa aactgcgtga gctggaaagc atttcgccga acgccgccgc gacgcaagtg
    60661 gatggtacgc agatgtcttt gctgtcagtg ccagaagaaa cttcgcctgc ggtcgaagca
    60721 ctggaaaatc tcgacccgga ttcactgact ccgcgtcagg cgctggagtg gatttatcgc
    60781 ttgaagagtc tggtgtaata acaattcccg atagtctttt gctatcggga atattaacga
    60841 caactgacga atcaaataaa aataccctgt ataataggaa agcttatttt acagggtaaa
    60901 accatgccat ctacacgcta tcaaaaaatc aatgcccatc actatcgcca tatatgggtc
    60961 gttggtgata ttcatggtga atatcagtta ttacaatccc gcttacatca actctctttt
    61021 ttccccgaaa ccgacttact tatttctgtc ggcgataata ttgatcgtgg gccggagagt
    61081 cttgacgtcc tgcgcctgct aaaccagccc tggtttatct ccgttaaagg caaccacgaa
    61141 gcaatggcgc tggatgcgtt cgcgaccggc gatggcaata tgtggcttgc cagcggtggt
    61201 gactagtttt tcgatttaaa tgattgcaac aagaggcaat agatctgttg ctgaaattcc
    61261 atcaccttcc acatattatt gaaatcacta acgacataat aaaatatgtc atcgcacatg
    61321 cagattatcc gggtgatgaa tatctctttg gcaaagaaat agcggagagc gaattactct
    61381 ggcctgttta tcgtgtgcag aaatcgctta atggcgagtt acaacaaata aacggcgctg
    61441 attattttat atttggacat atgatgtttg ataacattca gacgttcgct aaccagattt
    61501 atattgatac cggatcgccg aaaagtgggc ggctgtcatt ttataaaata aggtagtctc
    61561 atgcttcttc tgtgaagcat gagtaaccag atgttattgc aggccattat tcatttttcg
    61621 ctaccagcaa agagagatcc tgcttcgcca gcgcgcgact ggcactctcc ggcaaaccgt
    61681 cgtctgtaat aatctgatca aactcgctta atggtaacgc cagccatgtc gccacctgac
    61741 catatttcgt cgcatcacag accaaaactc gctggcggct agcactggca atcgcccgtt
    61801 tcaccgtgac tttatcttcc gctggcgtag aaatcccccg cacactccat gacgatgcag
    61861 aaataaaagc ctgatcaatc atcaggctgc gcagcatggt cgcagcggct tccccgacac
    61921 aggaacggtt ttcccgacac actgcaccgc cagtgtgaat aattgtgcaa ttactgttgt
    61981 cgagcaagta gttcgcaata acgaaatcgt ttgtgaccac agtcagtgac tccatgtgaa
    62041 tcagatgctg tgctatcgct aacgtggtcg ttcccgcatc cagatagata caacttcccg
    62101 gctgaacaag acttgccgcc agcttgccaa tagccgcttt ttgcgtcatt gccagcgcag
    62161 tttttacctg atgagaaggt tcatgcgcca cgcgtcccgg agactggacg cctccggaca
    62221 ccagcacaac ggctccctgc tgctccagtt tttgtaaatc ccgacgaatg gtcatatgtg
    62281 acacattaat tctgtccgtt agttcagcaa tactgacaat gcctttttca gctaccatct
    62341 caaggatgat ttggcgacgc tctacgggta tcaacttttg ctccttcctt tgtcctgctg
    62401 acattctacg ctatttgcct gcgaaacgtg cgcggcgcaa ctaacgctta gttcacataa
    62461 aataacacaa aatgttaatt tatgtgaatc agctcaccat atcgttatct cccagcgctt
    62521 atattcacaa tgtcaaacaa aatatcactt aaattaacaa ggagagcaga tgaaaacggg
    62581 atctgagttt catgtcggta tcgttggctt agggtcaatg ggaatgggag cagcactgtc
    62641 atgtgtccgc gcaggttttt ctacctgggg cgcagacctg aacagcaatg cctgcgctac
    62701 gttgaaagag gcaggtgctt gcggggtttc tgataacgcc gcgacgtttg ccgaaaaact
    62761 ggacgctctg ctggtgctgg tggtcaatgc ggcccaggtt aaacaggtgc tgtttggtga
    62821 aacaggcgtt gcacaacatc tgaaacccgg tacggcagta atggtttctt ccactatcgc
    62881 tagtgctgat gcgcaagaaa ttgctaccgc tctggctgga ttcgatctgg aaatgctgga
    62941 tgcgccagtt tctggtggtg cagtaaaagc cgctaacggt gaaatgactg tcatggcctc
    63001 cggtagcgat attgcctttg aacgactggc acccgtgctg gaagccgttg ccggaaaagt
    63061 ttatcgcata ggtgcagaac cgggactagg ttcgaccgta aaaattattc accagttgtt
    63121 agcgggcgta catattgctg ccggagccga agcgatggca cttgcagccc gtgcggggat
    63181 cccgctggat gtgatgtatg acgtcgtgac caatgccgcc ggaaattcct ggatgttcga
    63241 aaaccggatg cgtcatgtgg tggatggcga ttacaccccg cattcagccg tcgatatttt
    63301 tgttaaggat cttggtctgg ttgccgatac agccaaagcc ctgcacttcc cgctgccatt
    63361 ggcctcaaca gcattgaata tgttcaccag cgccagtaac gcgggttacg ggaaagaaga
    63421 cgatagcgca gttatcaaga ttttctctgg catcactcta ccgggagcga aatcatgatc
    63481 aagattggcg ttatcgccga tgattttacc ggcgcgacgg atatcgccag ttttctggtg
    63541 gaaaacggtc taccaacggt acaaattaac ggtgttccaa caggtaaaat gccggaagca
    63601 atcgacgcac tggtgatcag cctgaaaacg cgctcctgtc cggtggttga agccacacag
    63661 caatcgctgg cggctctgag ctggttgcaa cagcaaggtt gcaaacagat ctatttcaaa
    63721 tacagctcta ctttcgacag tacggcgaaa ggtaatattg gcccggttac cgatgcatta
    63781 atggatgctc tcgacacgcc gtttacggtc ttctctccgg ccctgccggt caacggacgt
    63841 acggtttatc aggggtattt gttcgtaatg aatcaactgc tggccgaatc cgggatgcgc
    63901 catcacccgg taaatcccat gaccaacagc tatcttcccc gtctggttga agcgcaatcc
    63961 acagggcgct gcggcgtcgt ttcggcacat gttttcgaac aaggtgtgga tgccgttcgt
    64021 caagagctgg ctcgcttaca gcaagagggc taccgctacg cggtgcttga tgcgctgacc
    64081 gaacaccatc tggaaattca gggagaagcc ttgcgcgatg ccccactggt aacgggcggt
    64141 tctggtctgg cgattggcct ggcccggcag tgggcacaag aaaacggtaa ccaggctcgc
    64201 gaagcagggc gtccgctcgc tgggcgcggc gtagtgctct ccggttcatg ctctcaaatg
    64261 accaaccgct aggtggcaca ttaccgtcaa attgcaccag cccgtgaagt tgatgtggca
    64321 cgctgcctct caaccgaaac tctggccgct tatgcacacg aactggcaga gtgggttctg
    64381 ggccaggaaa gtgtacttgc tccactggtt tttgccaccg ccagcactga cgcattggca
    64441 gcaattcaac agcaatacgg tgcacaaaaa gccagtcagg cagtagaaac tctgttttct
    64501 caactagcgg cgcggttagc agcggaaggc atgacacgct ttattgtcgc aggtggtgag
    64561 acctccggcg tagtcacaca gagcctgggc ataaaagggt ttcatattgg cccaaccatt
    64621 tcccccggcg tgccgtgggt aaacgcactg gataagcctg tctcactcgc ccttaaatct
    64681 ggcaacttcg gtgatgaagc ctttttttca cgagcccaaa gagagttttt atcatgagcg
    64741 atttcgcaaa agtagagcag tctttgcgag aggagatgac gcggattgcc agttcattct
    64801 ttcagcgcgg ctatgcaacc ggttcggctg gcaatctgtc gctgctttta cctgacggga
    64861 atttactggc gacaccgaca ggttcatgcc tgggcaatct cgatccgcag cggctttcca
    64921 aagtcgccgc ggatggcgaa tggttaagtg gtgacaaacc ctcgaaagag gtgctctttc
    64981 atctggcgct gtatcgcaac aatccgcgct gtaaagcggt ggtgcatttg cacagcacat
    65041 ggtcgacggc gctttcctgc ctgcaagggc tggacagcag caacgttatt cgtccgttca
    65101 caccatacgt ggtgatgcgg atgggaaatg tcccgctggt gccctattac cgaccgggcg
    65161 ataaacgcat cgcacaggat ctggcggaac tggcagcaga caatcaggct tttttactgg
    65221 caaatcatgg cccagtggtt tgcggtgaaa gcctgcaaga agccgccaac aatatggaag
    65281 agctggagga aacggcaaag ctgattttta ttctcggtga ccgcccgatc cgttatctga
    65341 ccgcagg
//
LOCUS       DAJTYG010000010        65142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTYG010000010 DAJTYG010000000
VERSION     DAJTYG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.010"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO568_000701"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO568_000701"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6640176.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO568_000702"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO568_000702"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6640177.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO568_000703"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO568_000703"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6640178.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="OO568_000704"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="OO568_000704"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6640179.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPTRGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="OO568_000705"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="OO568_000705"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6640180.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLHQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="OO568_000706"
     CDS             7826..9559
                     /locus_tag="OO568_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6640181.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="OO568_000707"
     CDS             9708..10559
                     /locus_tag="OO568_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6640182.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLRGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="OO568_000708"
     CDS             complement(10546..10887)
                     /locus_tag="OO568_000708"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6640183.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="OO568_000709"
     CDS             complement(10889..11767)
                     /locus_tag="OO568_000709"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6640184.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCSWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="OO568_000710"
     CDS             complement(11733..14030)
                     /locus_tag="OO568_000710"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6640185.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQGRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGHDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="OO568_000711"
     CDS             complement(14081..14401)
                     /locus_tag="OO568_000711"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6640186.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="OO568_000712"
     CDS             complement(14416..15495)
                     /locus_tag="OO568_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6640187.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="OO568_000713"
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="OO568_000713"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6640188.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="OO568_000714"
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="OO568_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6640189.1"
                     /translation="MELYLDTANVTEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAEGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPCQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            18990..20082
                     /gene="gldA"
                     /locus_tag="OO568_000715"
                     /pseudo
     CDS             18990..20082
                     /gene="gldA"
                     /locus_tag="OO568_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20357..20974
                     /locus_tag="OO568_000716"
     CDS             20357..20974
                     /locus_tag="OO568_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR6640190.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQECGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21001..21906)
                     /gene="yijE"
                     /locus_tag="OO568_000717"
     CDS             complement(21001..21906)
                     /gene="yijE"
                     /locus_tag="OO568_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6640191.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22000..24180)
                     /gene="katG"
                     /locus_tag="OO568_000718"
     CDS             complement(22000..24180)
                     /gene="katG"
                     /locus_tag="OO568_000718"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6640192.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGVNGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24509..25399)
                     /gene="metF"
                     /locus_tag="OO568_000719"
     CDS             complement(24509..25399)
                     /gene="metF"
                     /locus_tag="OO568_000719"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6640193.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGICHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25748..28180)
                     /gene="metL"
                     /locus_tag="OO568_000720"
     CDS             complement(25748..28180)
                     /gene="metL"
                     /locus_tag="OO568_000720"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6640194.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28183..29343)
                     /gene="metB"
                     /locus_tag="OO568_000721"
     CDS             complement(28183..29343)
                     /gene="metB"
                     /locus_tag="OO568_000721"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6640195.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29620..29937
                     /gene="metJ"
                     /locus_tag="OO568_000722"
     CDS             29620..29937
                     /gene="metJ"
                     /locus_tag="OO568_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6640196.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30121..30729
                     /locus_tag="OO568_000723"
                     /pseudo
     CDS             30121..30729
                     /locus_tag="OO568_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
     gene            complement(30790..31002)
                     /gene="rpmE"
                     /locus_tag="OO568_000724"
     CDS             complement(30790..31002)
                     /gene="rpmE"
                     /locus_tag="OO568_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6640197.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31205..33403
                     /gene="priA"
                     /locus_tag="OO568_000725"
     CDS             31205..33403
                     /gene="priA"
                     /locus_tag="OO568_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6640198.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33559..34584
                     /gene="cytR"
                     /locus_tag="OO568_000726"
     CDS             33559..34584
                     /gene="cytR"
                     /locus_tag="OO568_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6640199.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34676..35635
                     /gene="ftsN"
                     /locus_tag="OO568_000727"
     CDS             34676..35635
                     /gene="ftsN"
                     /locus_tag="OO568_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6640200.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35728..36258
                     /gene="hslV"
                     /locus_tag="OO568_000728"
     CDS             35728..36258
                     /gene="hslV"
                     /locus_tag="OO568_000728"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6640201.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36268..37599
                     /gene="hslU"
                     /locus_tag="OO568_000729"
     CDS             36268..37599
                     /gene="hslU"
                     /locus_tag="OO568_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6640202.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37666..38592
                     /gene="menA"
                     /locus_tag="OO568_000730"
     CDS             37666..38592
                     /gene="menA"
                     /locus_tag="OO568_000730"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6640203.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38685..39170
                     /gene="rraA"
                     /locus_tag="OO568_000731"
     CDS             38685..39170
                     /gene="rraA"
                     /locus_tag="OO568_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6640204.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39255..39500)
                     /gene="zapB"
                     /locus_tag="OO568_000732"
     CDS             complement(39255..39500)
                     /gene="zapB"
                     /locus_tag="OO568_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6640205.1"
                     /translation="MTMSLEVFDKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39926..40771
                     /gene="glpF"
                     /locus_tag="OO568_000733"
                     /pseudo
     CDS             39926..40771
                     /gene="glpF"
                     /locus_tag="OO568_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40811..42301
                     /gene="glpK"
                     /locus_tag="OO568_000734"
     CDS             40811..42301
                     /gene="glpK"
                     /locus_tag="OO568_000734"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6640206.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYISSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEENYALEGAVFMAGTSIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLSAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42531..43541
                     /gene="glpX"
                     /locus_tag="OO568_000735"
     CDS             42531..43541
                     /gene="glpX"
                     /locus_tag="OO568_000735"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6640207.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43638..44384
                     /gene="fpr"
                     /locus_tag="OO568_000736"
     CDS             43638..44384
                     /gene="fpr"
                     /locus_tag="OO568_000736"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6640208.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44389..44817)
                     /gene="uspD"
                     /locus_tag="OO568_000737"
     CDS             complement(44389..44817)
                     /gene="uspD"
                     /locus_tag="OO568_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6640209.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44844..45143)
                     /locus_tag="OO568_000738"
     CDS             complement(44844..45143)
                     /locus_tag="OO568_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6640210.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAAFSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45355..45795)
                     /locus_tag="OO568_000739"
                     /pseudo
     CDS             complement(45355..45795)
                     /locus_tag="OO568_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45896..46495
                     /locus_tag="OO568_000740"
     CDS             45896..46495
                     /locus_tag="OO568_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6640211.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46603..47370
                     /gene="tpiA"
                     /locus_tag="OO568_000741"
     CDS             46603..47370
                     /gene="tpiA"
                     /locus_tag="OO568_000741"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6640212.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47425..48180)
                     /gene="cdh"
                     /locus_tag="OO568_000742"
     CDS             complement(47425..48180)
                     /gene="cdh"
                     /locus_tag="OO568_000742"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6640213.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMSTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48287..49276)
                     /gene="sbp"
                     /locus_tag="OO568_000743"
     CDS             complement(48287..49276)
                     /gene="sbp"
                     /locus_tag="OO568_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6640214.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49596..50558)
                     /gene="pfkA"
                     /locus_tag="OO568_000744"
     CDS             complement(49596..50558)
                     /gene="pfkA"
                     /locus_tag="OO568_000744"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6640215.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50739..51641)
                     /gene="fieF"
                     /locus_tag="OO568_000745"
     CDS             complement(50739..51641)
                     /gene="fieF"
                     /locus_tag="OO568_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6640216.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51789..52289)
                     /gene="cpxP"
                     /locus_tag="OO568_000746"
     CDS             complement(51789..52289)
                     /gene="cpxP"
                     /locus_tag="OO568_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6640217.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52439..53137
                     /gene="cpxR"
                     /locus_tag="OO568_000747"
     CDS             52439..53137
                     /gene="cpxR"
                     /locus_tag="OO568_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6640218.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53134..54507
                     /gene="cpxA"
                     /locus_tag="OO568_000748"
     CDS             53134..54507
                     /gene="cpxA"
                     /locus_tag="OO568_000748"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6640219.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTKLLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54613..55287)
                     /gene="yiiM"
                     /locus_tag="OO568_000749"
     CDS             complement(54613..55287)
                     /gene="yiiM"
                     /locus_tag="OO568_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6640220.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(55436..56419)
                     /gene="kdgT"
                     /locus_tag="OO568_000750"
     CDS             complement(55436..56419)
                     /gene="kdgT"
                     /locus_tag="OO568_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR6640221.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAIIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(56679..57299)
                     /gene="sodA"
                     /locus_tag="OO568_000751"
     CDS             complement(56679..57299)
                     /gene="sodA"
                     /locus_tag="OO568_000751"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6640222.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            57584..58618
                     /gene="rhaT"
                     /locus_tag="OO568_000752"
                     /pseudo
     CDS             57584..58618
                     /gene="rhaT"
                     /locus_tag="OO568_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(58587..59480)
                     /gene="rhaR"
                     /locus_tag="OO568_000753"
     CDS             complement(58587..59480)
                     /gene="rhaR"
                     /locus_tag="OO568_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR6640223.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRISIRRKINSPCRCRRC"
     gene            complement(59536..60372)
                     /gene="rhaS"
                     /locus_tag="OO568_000754"
                     /pseudo
     CDS             complement(59536..60372)
                     /gene="rhaS"
                     /locus_tag="OO568_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
     gene            60660..62129
                     /gene="rhaB"
                     /locus_tag="OO568_000755"
     CDS             60660..62129
                     /gene="rhaB"
                     /locus_tag="OO568_000755"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6640224.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELITLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            62126..63385
                     /gene="rhaA"
                     /locus_tag="OO568_000756"
     CDS             62126..63385
                     /gene="rhaA"
                     /locus_tag="OO568_000756"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6640225.1"
                     /translation="MTTQLEQAWELAKQRFAAVRIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            63627..64451
                     /gene="rhaD"
                     /locus_tag="OO568_000757"
     CDS             63627..64451
                     /gene="rhaD"
                     /locus_tag="OO568_000757"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR6640226.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLAVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            64461..64775
                     /gene="rhaM"
                     /locus_tag="OO568_000758"
     CDS             64461..64775
                     /gene="rhaM"
                     /locus_tag="OO568_000758"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6640227.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            65076..>65142
                     /locus_tag="OO568_000759"
     CDS             65076..>65142
                     /locus_tag="OO568_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="with FrvBRX is part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system involved in the transport and phosphorylation of
                     sugars; possibly responsible for the sugar specificity of
                     the system; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6640228.1"
                     /translation="MAALTASCIDLNIQGNGAYSVL"
BASE COUNT        15885 a        17549 c        16663 g        15045 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccaacacg cggagttaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acacaaccag ctgatgcgtc
     5521 gcctgcacca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctaggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttcct ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atagcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgcgcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac attttgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgagcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc agcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cgtgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggccctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccaatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggagtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt cgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctggctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gccgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcacag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaag agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgtgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcagtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctctaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctcac
    19741 gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca cggtgaaaaa
    19801 gtggcatttg gtacgctgac gcagctggta ctggaaaacg cgccggtgga ggaaatcgaa
    19861 accgtagctg cacttagcca tgcggtaggt ttgccaataa ctctcgctca actggatatt
    19921 aaagaagatg tcccggcgaa aatgcgcatt gttgcaaaag catcttgtgc agaaggtgaa
    19981 accatccaca acatgcctgg cggcgcgacg ccagatcagg tttacgccgc actgctggta
    20041 gctgaccagt acggtcagcg tttcctgcaa gagtgggaat aacctactcc aaactcccgg
    20101 cttgtccggg agtttgaacg caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa
    20161 tctctgcaat atattgaatt tgcgtgcttt tgtaggccgg ataaggcgtt cactccgcat
    20221 ccggcactct gtgccagtaa tctgaaactc ccacgttgtt caggagtttt ttatccgctt
    20281 ctggacattt tctccacaga acaatcgtta gcccggaagg tcgaatctgt gactcttatt
    20341 ttcacggacg aaccagatga aagcttcact ggcgctgctc agcctgctaa cggcatttac
    20401 cagccactcc ttaaaatctc ccgccgttcc accaaccgtg gtacaaatcc aggccaatac
    20461 caaccttgcc atcgcagacg gtgccagaca gcaaattggc agcacgctat tttacgatcc
    20521 ggcgtatatg cagcttacct atcctggcgg tgatgttccg caagaatgcg gtgtatgttc
    20581 cgatgtggtg atccgcgcat tgcgcagcca gaaggtcgat ttgcagaaac tggttcacga
    20641 agatatggcg aagaattttg ctgagtaccc gcaaaaatgg aagttaaagc gcccggacag
    20701 caacatcgat caccgtcggg tgcctaatct ggaaacctgg tttagccgcc acaacaaaac
    20761 acgccccatc agtaaaaatg ccggtgacta tcaagcgggc gatattgtct cctggcgatt
    20821 agataacggg ctggcgcata ttggggtggc gtcagatggc ttcgcccgcg acggtacgcc
    20881 gctggtgata cataacattg gcgcaggcgc gcaggaagaa gatgtgctgt ttagctggcg
    20941 gatggtgggg cattatcgtt attttgttaa ataagagatc gccccacatg aagaaaaaat
    21001 tcagatgctt tttacactga cagcttcttt tttcttacgg ctcaccagcg ccagtgccag
    21061 cacaatcagc acaataccgc taccttcaac ggcccccgga ttctcaccga gcagccacca
    21121 ggaaaagagt acgccacaaa cgggaacggc cagtgtgctt aagctggcaa tactggcagg
    21181 caagtttttc agcacaaaca gccataagct ccacgctagc gccgtcgcca gaatcgcact
    21241 gtaggccagc gcccagaaca cggtgggctg ccagtcaatt tcacgttgcg gcaccagtaa
    21301 agcgaccaca ctcatcacca gcgccgcata cagcatctgc caggatgtta acgataataa
    21361 atccacgcgc ggatgacggg cgtataggcg tttagcaaca atcgcgctcg ccccccaact
    21421 aacaccggag aggattgcca gcatggcact tttcatcgaa gagaaatcga gttgccacgg
    21481 ctgcaacacc aaaaataagc cgaaagcggc aatcagaatc gcgaaatatt gcccacgtcg
    21541 caggcgttca ccgagaaaca acgcggcgaa aatcaccacc cagaacggca tggtatagct
    21601 caggatcgcc accttccccg cacctccgct gaccaacgcc cactgcgcca gaccaaccat
    21661 cccgcaggtt tgtaacaggg caatggctaa ggtgtattta aacggcgtcg ggcgcattcc
    21721 gcgaccacgt aataaaagga cgatgaataa aacgagagcg ccgaaaatgc agcgtaaggc
    21781 ggtaaagtcg aaggcaccga tgtaactggt gacttgcttc atgaaaatcc agctataact
    21841 ccagataagt gtgagcacaa ccaggccact gattgccagt gggttgctct ttcctgcggc
    21901 agacatgaga gatccagtgt gtagttttcc tgtggacact atacgccggt aaagaaactt
    21961 cagcgactgc ctgaaagaca gtcgctgaag aaggtcagat tacagcaggt cgaaacggtc
    22021 gaggttcatc actttcaccc atgccgctac gaagtcttta acaaacttct cgtgggcatc
    22081 gctgctggcg taaacttccg ctactgcacg caggacagag ttagaaccaa ataccagatc
    22141 cgcacggctg gctgtgtatt tcacttcgcc ggtttcgcgg tcacgtcctt cgaacagctc
    22201 tttcgattcg tcagtcgctt tccactcgta acgcatatcc agcaagttca cgaagaagtc
    22261 attgctcaat acgccaacgc ggtcagtgaa gacgccgttt ttgctgccat cgaagttggc
    22321 acccagtaca cgcatcccgc ccaccagcgc agtcatttcc ggcgcggtca gcgtcagttg
    22381 ctgtgctttg tcgatcagca gtgactcggt ggtggaaacg tccagacgag cgcgatagtt
    22441 acggaaaccg tcagcaattg gctccagcag ctcaaacatc tcaatgtcag tctgatcctg
    22501 acgcgcatca acgcgacccg gcgcaaacgg tacatgaatg ctcaaacctg cggcgcttgc
    22561 ggctttctca acaccaacca caccagccag cacgatgata tccgccagcg aggctttacc
    22621 agactctttc tggattttct ccagaacagg cagagcacga acggctgcgg cgttcacatc
    22681 ccagtcgcgc tgcggcatta atgccagacg cgcaccgttg acaccaccgc gtttgtcgcc
    22741 accacggaag gtagaagcag atgcccaggc caccgatacc agctcactaa cagacagacc
    22801 agaatccgca atcgcgaatt tcagatcgat aatgtcctgc tcggtcgggt tgtagatcgg
    22861 ctgcggcagc ggatcttgcc agatcagatc ttctttcggc acttccggcc cgatgtagcg
    22921 agatttcggc cccatatccc tgtgcgtcag tttgaaccag gcacgggcaa aggcttcgtt
    22981 gaacgcctgc ggatcgttga ggaaacgacg agagatcttc tcgaactcag gatcaaaacg
    23041 cagcgtcagg tcggtcacca gcattgtcgg tttacgtttc ttcgacggat cgaacggatc
    23101 cgggataatt tccggtgcgt ctaccgcttc gaactggatt gctccagccg gactgcgggt
    23161 ctgtacccac tcatacttga acagattctc gaagaaatag ttgctccact gggtcggcgt
    23221 ctgggtccag actacttcca gaccagaggt aatggcatct gcgccaacgc cgctgccgta
    23281 agtgctcgcc cagcctaaac cttgttcttc aatcggtgca gcttctggat caggacctac
    23341 gtttgatgtc ggaccggcac cgtgggtttt acccagcgta tgaccacccg caatcagcgc
    23401 cacggtttct tcgtcgttca tgcccatgtt gccgaaggtc gcgcggatag ctgctgccgc
    23461 agaaagcggt tcgccgctgt gatccgggcc ttccgggtta acgtaaatca ggcccatctc
    23521 ggttgcaccc agcggtgctt tcgccagcgc ttccggatga cggtgagtca gccaggcttt
    23581 ttcatcaccc cagttaacat ccagatccgg ttcccagacg tcttcacgac cggcaccaaa
    23641 accgaaggta cggaagccgg agttttccag cgccacgtta cctgcgagga taaacaggtc
    23701 ggcccaggag attttctgac catatttctg tttgattggc cacaacaggc gacgcgcttt
    23761 atcgaggctt acgttatccg gccaggagtt cagcggtgca aaacgttgct gaccacgacc
    23821 cgcgccgccg cgtccatcga ttgaacggta agtacctgcg ccgtgccaag ccatacgaat
    23881 aaacagaccg gcgtagctgc cccagtcggc tggccaccac ggctgagatt ctgtcagcag
    23941 ggctttcaga tcttttttca ggccgtagta atctaatttg ctgaattctt tgcggtagtc
    24001 aaagtcctca cccagcgggt tagaacgatt agaatgttgg tttaacaggt caacacggag
    24061 ttgatttggc caccagtcgc gagtggttgt gcccgccccc gcgctctggt cgtgaccgcc
    24121 ctgatggaac gggcatttgc cagtggctgt ggtgttatgg atatcgtctg acgtgctcat
    24181 caatgtgctc ccctctacag tgttaccgtt acgatacaca gcgttagaga gaagttataa
    24241 ttgaattaat ccacggatgc gatagttgag atcttacata ttgttggtta aagagatgta
    24301 gatcaaattg atcgtaatta agcttttttg attatgtccg aattcggaca aatgctttat
    24361 aaaaaagggt tttagcttca tatccttcaa gtaatgaggg agaaaacaag gctgacaacc
    24421 ttattttccc gccctcattt cgaggcagca ttttgtgctc tgtttaaaat ttgtgatcac
    24481 tgtgtgattt tcacaaaagc cacactattt ataaaccagg tcgaacaccc agcgtatggc
    24541 aaatcgcgta actcatttca gcgcggttaa gcgtatagaa gtggaaatct ttcactcctt
    24601 cacggcttaa aatcttcacc atatccatgg caatattcgc gcccaccagt ttgcgggttt
    24661 cggcatcgtc atccagcccg tcaaacattt gcgccatcca cgccggaata cgcacgttgg
    24721 tcatgtcggc aaatttcttc gcctgtttaa agttagatac cggcaaaatg cccgggatga
    24781 tttccacatc aatgcccgcc gatacacagc ggtcacgaaa acgcaggtag ctttcgacat
    24841 cgaagaagaa ctgagtaatc gcgcggttgg ctccggcatc cactttgcgt ttcagattaa
    24901 gcaaatccgc ctgagcgctt tttgcttccg ggtgaacttc cggatacgcc gccacggata
    24961 tatcgaaatc tgccacttct tttaacagcg tcaccaggtc agaagcatac atttctggct
    25021 taccacttcc cggcggcaga tcgccacgca gtgccacgat atgacaaata ccgttattcc
    25081 agtagtcgcg tgcaatggtg cgcagctcgt cgggcgtcgc atcaatgcaa gtaagatgcg
    25141 gtgccgcttc cagaccagtg cgatctttaa tgcctttaat aatgctgtgc gtacggtcgc
    25201 gctcgccgga gttcgcgcca taggtcaccg atacaaactt cggtttcagg ctgctaaggc
    25261 gatcgatgga gttccacagg gtctgctcca tttcactggt acgcggcggg aaaaactcga
    25321 acgaaacgtt aatctgcccc tggacttctg ccaggctctg attcagggca tcccgctggc
    25381 tggcgtgaaa aaagctcata ccttacctca tcaatcgctt gtagttatat gttgtgttgt
    25441 gcacatctat ccatttagac gtccagatgt aaagatgaag gaaaagccga agggcgtcaa
    25501 ccgaaaaatg accaatgatg gtgaggaaaa atcagggaag atgaaaaaac ttcaggatgg
    25561 taagaaaaag aaaatccgat atgcaaagca aaccggactg caaatataaa ctaaccatgt
    25621 acttattgct tcttgccgga ttcgtcgtaa acgcttcatc cggcctaatt ctatgataat
    25681 tcaatatatt tcattagaaa ccatagaccc gaaaacatga gtaccgggca ttattaaatt
    25741 tctgaaatta caacaactgt gccagccggt tgatatccga ctgaatcgcc ccggcggtga
    25801 cgtcgcgccc agcgccaggc ccacggatca ccagcggatt atcgcggtac cagcggcttt
    25861 cgatggcaaa gacgttatcg catggcagca gtgatgccag cggatggtct tcacgcaccg
    25921 cctccacgcc cacacgcgct ttgccgttgg catcgaaacg cgccacgtag cgcagcacca
    25981 gccccatttc gcgggccgct tccagccgtt gcaccatctg ctcgttaagt tcatcgccat
    26041 tttcaaagaa atggtcgatg ctgccacctt cgcaatgagc aggcaccagc gattccacac
    26101 gtacctggtc cggttcaatg ttgtaacccg cttcacgcgc cagaatcacc agcttgcgca
    26161 ttacgtcttt gccggagagg tcgtcacgcg gatcaggctc ggttaagccc tgctgccacg
    26221 cctgatccac cagctcggta aacggcacgc taccgtcgaa ttgcaggaac agccaggaga
    26281 gcgtgccgga gaagatccca ctgatcgaca aaatggtatc gccgctgtcg atcagatcgc
    26341 gcacggtatg gttgatcggc aagcccgcac caacggtggc gttgtatagc cagtgacgcc
    26401 cggttttttc gaaggcgtcg tggatctggc gatatttatt gctgtcgctt gctcccgcca
    26461 gtttgttggc actgatgacg tggaaaccgt ggctggcgaa atcgagatac tgatcagcaa
    26521 gctgctggct ggcggtaacg tccaacacca ctaaatcatc atacggatgg gcgcgcatcc
    26581 acaggaaaag cgactcttca tcctgctcga ccgcttcatc gttgaagaag gctaacgcgc
    26641 ggctggcgtc cagcccgtca taactcaaca gactgcggcg gctgtccacc acgcctgcca
    26701 gcacaaattc aaagccagta cgcgccgaaa gcgtgctctg ctcgcgggcg aacagttcca
    26761 gccagcgaga gccgatattg cctttgccga acaataccag gccgatgcgt ttttctgcgc
    26821 ggaagacgga ctgatgcagc ccctgaatca ggctttcggt cgggccggtg cgcagtactg
    26881 ccaccagact gatgccgtca tcggactgcc acgtaaactc gaccggctgg cctttcagtt
    26941 gctgccagaa gcggtggcaa tgcagcgggt tacgggtgac acccgccccg accatcgcca
    27001 ccagcgccag cccctgacgc aggcgcagtt ctccaggtaa tcccgcttcg tcgaggattt
    27061 tcagcgcact gtcggccact tctgaggtgt agcaaaattg cagcaactgg cgatcgttat
    27121 gcacgccaac cgccagtgga cgcacctggg cacgtttcag gatctggtcg atctctttat
    27181 gcgccagttt gaaatcctga ctggcgggca cctgaaactc aatcaaacag acatcatcgt
    27241 ggctggtgac aatacgcgca ccagtaccgg aggccagcac gcgttcaatg cgcgtggaac
    27301 cttgatccgg cgtgtagcta cagcgcagct gcaggtcgat ttcgctgcca gaaactggct
    27361 gtaaagtacg ggcgtgaaga acgggagccg ccagacgcgc cagttcgctg gcctcatcca
    27421 gacgcagcaa cggcagcagg caggcatctt tcactttacg cgggtcggca ctgtataccc
    27481 cggcgacgtc gctccagatg gttacacgag aaacacccgc cagcgcaccg atttgtgtcg
    27541 cggaatagtc ggaaccgtta cgccccagca acaccgtttc accggcgttg ttacggctga
    27601 taaacccggt caccaccaga cgtttgctcg gatgttgtac cagtagctgt tgcagcaacg
    27661 ggtaagaaag cccttcatca acctgcggtt gcgcagcgcg ttcagcgcgt aaaaactcac
    27721 gggcatcaag ccaggccgct ggcagccctt gttgattaag taccgcagac atcaggcgcg
    27781 ccgaccacac ttcaccgtgg cccaccactt ccgcatacac cgcatcgtta ataccactgt
    27841 cgagcaacgc cgccaggcgc tcaagatcgc tgacaaaagc gctaatgagg ctatcggctt
    27901 cttcagcggg tagcagaccg ctaatcagat cgcactgata gcgacgcagc gtttgttgaa
    27961 cctgatgcgc agagagacga tcggtctggc ttagtttcaa ccagttaatc aactggttag
    28021 tggtgctacc ggcggcggaa accaccatca tatcgtcagg ctgagagaac tccgccataa
    28081 tgcccgcgac acgcaaataa cacttcacat cagccagact actgccacca aatttatgca
    28141 gctgacgacc tttcgcccct gcctgcgcaa tcacactcat ttttacccct tgtttgcagc
    28201 ccggaagcca ttttccaggt cggcaattaa atcttcgcca tcttcaatac cggtggagat
    28261 acgcagcagc gtctcggaga tcccggcggc agcacgcgct tctggtgcca tgcctgcatg
    28321 tgtcatggtt gcggcgtgag agattaaact ttccactccc cctaatgatt ccgccagcgt
    28381 aaacaacgac agcccgccca ggaaacgaca cagcgtctgc tcatcgccat ccagttcaaa
    28441 actcaacatt gcgccaaagc ctttttgctg acgcgcggca atttcatgcc cctgattttc
    28501 cggcagcgac gggtgataca gttttttcac caacggctgg gtttgcagat atttcacaat
    28561 cgcctgcgcg ttgcgctgcg ccagctccat acgcggcacc agcgttcgca acccgcgtag
    28621 cagcagatag ctgtcaaacg cgccgcccgt cacgccaata ttgtttgccc accaggcaag
    28681 ttcagtgaca acgtccgggt ctttagcaat caccacgcca gccaccacgt cagagtgacc
    28741 gttcagatat ttcgtacatg aatgcaacac cagatcggca cccaatgcca gcggattttg
    28801 taatgccggg cttaagaagg tgttatccac cacgctcacc gccccgactt cccttgccag
    28861 atggcagatt ttcgcaatat ccacgacgcg taacaatgga ttacttgggc tttctaccag
    28921 taccagtttg ggtttttctg ccagcgctgc ccgtaatgcc tgttcatcgc cttgatcaac
    28981 aaacaacacg cgatagcaac cgcgtttcgc cagactgtcg aacaggcgat agctaccgcc
    29041 gtagcagtcg tgcggcgcaa ccagcagatc gccaggtttc aaaaagacgg tcgttaccag
    29101 gtgaatcgcg gacatgccgg tattagtaag tactgcgcca gcaccgcctt ccagttctgc
    29161 cagcgcacgc tgaaccacat cgcgcgttgg gttgccgcga cgcgagtaat catgcgcgcg
    29221 cggttcatta aatccggtaa agttataggt gctggaaaga tggatgggag ggacaacgca
    29281 accatactgt tcgtcgtcat ttaacccgct acgcactgcg atggtggcct gtttacgcgt
    29341 catgtgatga agtttcctgg gctttgtcgg tgaaatgtca ggcaccagag taaacattgt
    29401 gttaatggac gtcaatacat ctggacatct aaacttcttt gcgtatagat tgagcaaatc
    29461 ccaaatagcc gttaaaatta tatgcattat cacgccgaca ggtgcattac acgatgtcac
    29521 ggtaacgcct gtacggtaaa ctatgcgggt ttacggtcag taccaacata aattgtgggg
    29581 tctggtctca atttattgac gaagaggatt aagtatctca tggctgaatg gagcggcgaa
    29641 tatatcagcc catacgctga gcacggcaag aagagtgaac aagtcaaaaa gattacggtt
    29701 tccattcctc ttaaggtgtt aaaaatcctc accgatgaac gcacgcgtcg tcaggtgaac
    29761 aacctgcgtc acgctaccaa cagcgagctg ctgtgcgaag cgtttctgca tgcctttacc
    29821 gggcaacctt tgccggatga tgccgatctg cgtaaagagc gcagcgacga aatcccggaa
    29881 gcggcaaaag agatcatgcg tgagatgggg attaacccgg agacgtggga atactaaagc
    29941 ggaaaagagc ggcgcggagt ggaatcgcct gatgcactac gcttatcagg cctacgtcat
    30001 attgcaattt attgaatttg cacgaacttg taggccggat aaggcgttca cgccgcatcc
    30061 ggcataaaca acgagcacgt tgtctgcgac ccaccgcttt ttatacatgg acgtttaact
    30121 atgaaaaaca ggctgctgat cctcagcctg ctggtttctg tacctgcctt tgcctagcag
    30181 ccgcaaactg gcgacatcat ctttcaaatc tctcgctcat cgcaaagtaa agcgatccaa
    30241 ctggcaaccc attccgatta cagccacacc ggtatgctgg tgatgcgtaa caaaaagccc
    30301 tacgtttttg aagcggtcgg cccggtgaaa tacaccccgc tcaagcagtg gattgcccat
    30361 ggtgaaaagg gcaactacgt tgttcgccgc gttgaaggcg gactgagtgt tgaacaacag
    30421 caaaaactga cgcaaacggc aaaacgttat ctcggtaaac cgtatgattt cagcttctca
    30481 tggagcgacg atcgccagta ctgttcggaa gtggtgtgga aggtttacca gaacgcgctg
    30541 ggaatgcgcg tgggcgaaca gcagaagttg aaagagtttg atctcagcaa cccgctggtc
    30601 caggcgaagc tcaaagaacg ctacggtaaa aatattccgc ttgaggaaac ggtagtttca
    30661 ccacaagccg ttttcgacgc gccacaactc accacggttg ccaaagagtg gccgctgttt
    30721 tcgtggtgat acgaccaaaa ttcaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga
    30781 aatccggtct tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc
    30841 accggtagca acatcacgct gtttgccagt gaagaacggg tggcacttgc tgcacacgtc
    30901 gaggttcagg tcatgaccaa cggtggagcg gattttcatt acgttaccgc aagagcagct
    30961 agcagtaatt tcttcgtatt tcgggtgaat atcttttttc atgggaaaac ctcggtttaa
    31021 ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc gatgttaaaa gtatagcttc
    31081 aatacgatca tttcgtacga aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac
    31141 tgatccgcac tcttctacgg caatgtgtat actaacccac cgaatttcaa gtcaggatga
    31201 tgctatgccc gttgcccacg ttgccttgcc cgttccgctt cctcgtacct ttgactatct
    31261 gctgccagaa ggcatgacgg ttaaagctgg gtgtcgcgtg cgcgtgccgt ttggcaaaca
    31321 gcaggagcgc atcgggattg tggtatcagt tagcgatgcc agcgaactgc cgctcactga
    31381 gctaaaagcg gtagtcgaag tgctggatgg tgagccggtg tttactcact ccgtctggcg
    31441 attgctgcta tgggcggcag attactatca tcatccgatt ggcgatgtgc tgtttcatgc
    31501 cttgccgatt ttactacgcc aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt
    31561 tgccactgaa caaggtcagg cggtggatct gaacagcctg aaacgctcac ccaagcaaca
    31621 acaggcgctg gcagcgttac ggcaaggcaa aatctggcgc gaccaggttg ccacgctcga
    31681 atttaatgat gccgcgttgc aggcgctgcg caaaaaaggt ctgtgtgatt tagcaagtga
    31741 aacaccagag tttagcgact ggcgaacgaa ctatgccgtt tctggtgagc ggttgcgatt
    31801 gaataccgaa caggccaccg ccgttggcgc aattcatagc gcggcagata ctttttctgc
    31861 ctggctgctg gcgggcgtta ccggttccgg taaaacggag gtttatctca gtgtactgga
    31921 aaacgtgctc gctcagggca aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc
    31981 gcaaactatc gcccgttttc gtgaacggtt taatgcgccc gtggaagttc tgcattccgg
    32041 tctgaacgac agcgagcgtc tttcggcgtg gctgaaagcg aaaaatggcg aggcggcgat
    32101 tgtgatcggc acccgctccg cgctgtttac gccgtttaaa aatctcggcg tgattgtcat
    32161 tgacgaagag cacgacagtt cctacaagca gcaggaaggc tggcgctatc atgcccgcga
    32221 cctggcggtg tatcgtgcgc atagcgagca aatcccgatt attcttggct ccgcaacgcc
    32281 cgcgctggaa acgttgtgca acgtccagca gaaaaaatac cgcctgctgc gcctgacgcg
    32341 ccgggcgggg aatgcgcgtc cggcaattca acacgtgctg gatttaaaag gtcagaaggt
    32401 gcaggcaggt ctggctccgg cgttaatcac ccgtatgcgc cagcatttac aggccaacaa
    32461 tcaggttatc ctctttctta accgccgtgg ctttgcgcct gcgctactgt gccacgactg
    32521 cggctggatt gccgaatgcc cacgttgcga tcactactac acgctgcatc aggcgcaaca
    32581 gcatttgcgc tgtcaccact gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg
    32641 cggttccacg cacctggtac ccgtggggct gggcaccgaa cagcttgaac agacgcttgc
    32701 gccgctgttc cctgatgtgc ccatttctcg tatcgaccgc gataccacca gccgcaaagg
    32761 ggcgctggaa cagcaactgg cggaagtaca tcgcggcggt gcgaggattt tgatcggcac
    32821 acaaatgctg gcgaaaggtc accactttcc ggatgtgacg ctggtggcgt tactggatgt
    32881 ggacggcgcg ctgttttctg ccgattttcg ctcggcagag cgtttcgctc agctttacac
    32941 ccaggtcgct ggtcgtgccg ggcgtgcggg taaacaaggc gaagtggtgc tgcaaacgca
    33001 ccatccggaa catcctctgt tgcaaacgtt gctctataaa ggctacgacg cctttgccga
    33061 acaggcgctg gctgagcgac ggatgatgca actgccgcca tggaccagcc atgtgattgt
    33121 gcgtgcggaa gatcataaca atcagcatgc gccgctgttc ctgcaacaac tgcgtaatct
    33181 gatcctctcc agtccgctgg cagacgacaa actgtgggtt ctcggtccgg ttccggcact
    33241 ggcacctaaa cgtggcggtc gctggcgctg gcagatattg ttgcagcacc cttcccgcgt
    33301 gcgcttgcaa cacatcatta gtggtacgct ggcgctcatc aatacaatac cggattcccg
    33361 caaggtgaaa tgggtactgg atgttgatcc gattgagggt taaaccgctt acgatgcgag
    33421 gcggatcgaa aaattcaata ttcatcacac ttttcatgaa aattctgtaa ccgttttcac
    33481 gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt
    33541 accaggcgag gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac
    33601 gttgccctca aggcaaaagt ctctacagcg actgtctccc gagcattaat gaatcccgat
    33661 aaagtctccc aggccacccg taatcgggtt gaaaaagcgg cccgggaagt gggttattta
    33721 ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc
    33781 ccggatatct gcgatccctt ctttagcgaa attattcgcg gtatcgaagt tacggcggca
    33841 aatcacggat atctggtgct gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc
    33901 tttatcgatt tgatcatcac caagcaaatt gatggcatgt tgctgctggg ttcaaggctg
    33961 ccgtttgatg ccagcattga ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa
    34021 tttgcaccgg aactggagct gcctaccgtt catatcgaca atctgaccgc cgcatttgat
    34081 gcagtaaatt atttatatga gcaagggcat aaacggattg gctgtatagc cggtcccgaa
    34141 gagatgccac tgtgtcacta ccgcctgcaa ggctatgttc aggcgctgcg tcgctgcgga
    34201 attatggttg atccacaata catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa
    34261 gcaatgcaac agctgcttga tcttccacaa ccgcctactg cagtcttctg ccatagcgat
    34321 gtgatggcgc tcggcgcact ttctcaggca aaacgccagg ggctgaaagt cccggaagac
    34381 ctttccataa tcggttttga taacatcgac ctgacgcaat tttgtgatcc gccgctgaca
    34441 accatcgcgc agccgcgtta cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa
    34501 atgcaggggc aacacgttgg cagtggctct cgtttaatgg actgcgaact tatcatccgg
    34561 ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc
    34621 ccgccgctta agtaacatgg cgggctgacg aacgaataaa tacagcgaaa cgatagtggc
    34681 acaacgagat tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc
    34741 acggaaaaag caacgaaatc tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc
    34801 cgttcttgtg acctttatcg gtggtctgta cttcattacg catcacaaga aagaagagtc
    34861 cgagacgctg caaagccaga aagtgaccgg aaacggacta ccaccaaaac cagaagaacg
    34921 ctggcgctac attaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc
    34981 ttctgccggt ggtgaagtga aaacgccgga gcaactgaca ccagaacaac gtcagcttct
    35041 tgaacaaatg caggctgata tgcgccagca gccaacgcag ctggttgagg ttccgtggaa
    35101 tgaacagacg ccggaacagc gtcagcaaac gctacaacgc cagcgtcagg cgcagcaatt
    35161 agcggagcag caacgcctgg ctcagcagtc gcgtacgact gaacaaagct ggcagcagca
    35221 gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta aaccagcttc
    35281 tactcagcag ccgtaccagg atctgctgca aacccctgcg cacacgaccg cgcagtcgaa
    35341 gccgcagcaa gctgcgccgg ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa
    35401 aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga
    35461 gacggtacgt gctcagctgg cgttcgaagg atttgactcg aaaatcacca ccaacaatgg
    35521 ctggaatcgt gtggtcattg gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa
    35581 tcggttgaag atggcgggtc atacaaactg cattcggctc gccgccgggg gttgaaaccc
    35641 tcaaaatccc ccccatctat aattgcatta tgccccgtac ttttgtacgg ggtttgtact
    35701 ctgtattcgt aaccaagggg tcagctcgtg acaactatag taagcgtacg ccgtaacggc
    35761 catgtggtca tcgctggtga tggtcaggcc acgttgggca ataccgtaat gaaaggcaac
    35821 gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg cgggctttgc gggcggtact
    35881 gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca tcagggccat
    35941 ctggttaaag ccgccgttga gctggcaaaa gactggcgta ccgatcgcat gctgcgcaaa
    36001 cttgaagcac tgctggcagt cgctgatgaa actgcatcgc ttatcatcac cggtaacggt
    36061 gacgtggtgc agccagaaaa cgatcttatt gctatcggct ccggcggccc ttacgcccag
    36121 gctgcggcgc gcgcgctgtt agaaaacact gaacttagcg cccgtgaaat tgctgaaaag
    36181 gcgttggata ttgcaggcga catttgcatc tataccaacc atttccacac catcgaagaa
    36241 ttaagctaca aagcgtaagg atctcccatg tctgaaatga ccccacgcga aatcgtcagc
    36301 gaactggata agcacatcat cggccaggac aacgccaagc gttctgtggc gattgctctg
    36361 cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt gaccccgaaa
    36421 aatatcctga tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct
    36481 aagctggcga atgcgccgtt catcaaagtt gaagcgacca aatttaccga agtgggctac
    36541 gtcggtaagg aagttgattc tattattcgc gatctgaccg atgccgccgt gaaaatggta
    36601 cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag aactggcaga agaacgtatt
    36661 ctcgacgtgc tgatcccacc tgctaaaaac aactggggac agactgaaca gcagcaggaa
    36721 ccttccgctg ctcgtcaggc attccgcaaa aaactgcgtg aaggccagct tgatgacaaa
    36781 gaaatcgaga tcgatcttgc cgcagcaccg atgggcgttg aaattatggc tcctccgggc
    36841 atggaagaga tgaccagcca gctgcagtcc atgttccaga acctgggcgg ccagaagcaa
    36901 aaagcgcgta agctgaaaat caaagacgcc atgaagctgc tgattgaaga agaagcggcg
    36961 aaactggtga acccggaaga gctgaagcaa gacgctatcg acgctgttga gcagcacggg
    37021 atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat
    37081 gtttctcgtg aaggcgttca gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc
    37141 accaaacacg ggatggtcaa aactgaccac attctgttta tcgcttctgg cgcgttccag
    37201 attgcgaaac cgtctgacct gatcccggaa ctgcaaggtc ggctgccaat ccgcgttgaa
    37261 ctgcaggcac tgaccaccag cgacttcgag cgtattctga ccgagccgaa tgcctctatc
    37321 accgtgcagt acaaagcact gatggcgact gaaggcgtaa atatcgagtt taccgactcc
    37381 ggtattaaac gcatcgcgga agcggcatgg caggtgaacg aatctaccga aaacatcggt
    37441 gctcgtcgtt tacacactgt tctggagcgt ttaatggaag agatttccta cgacgccagc
    37501 gatttaagcg gtcaaaatat cactattgac gcagattatg tgagcaaaca tctggatgcg
    37561 ttggtggcag atgaagatct gagccgtttt atcctataat cgcgttcaat cattttcatc
    37621 attgtttgat ggggctgaaa ggccccattt ttattggcgc gtattatgac tgaacaacaa
    37681 attagccgaa ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc cctcgccttt
    37741 gctgcaatta tcgtcgggac ggcgctggca tggtggcaag gtcacttcga tccgctggtc
    37801 gccctgctgg cactaattac cgccgggcta ttacagatcc tttctaacct cgccaatgat
    37861 tacggcgatg cggtaaaagg cagcgataaa cctgaccgca ttgggccgct acgcggcatg
    37921 caaaaagggg tcattaccca gcaaaagatg aaacgggcgc tcattattac cgtcgtgctc
    37981 atctgtctct ccgggctggc actggttgca gtggcatgcc atacgctggc cgattttgtc
    38041 ggtttcctga ttcttggcgg gttgtcgatc attgccgcta tcacctacac cgtgggcaat
    38101 cgtccttatg gttatatcgg tctgggtgat atttccgtac tggttttctt tggctggttg
    38161 agtgtcatgg ggagctggta tttacaggct catacattga ttccggcact gatccttccg
    38221 gcgaccgcat gcggcctgct ggcaacggca gtactgaata ttaataacct gcgtgatatc
    38281 aatagcgacc gcgaaaatgg caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg
    38341 cgtcgttatc atgcctgcct gctgatgggc tcgctggtgt gtctggcgct gtttaatctc
    38401 ttttcgctgc atagcctgtg gggctggctg ttcctgctgg cggcaccatt actggtgaag
    38461 caagcccgtt atgtgatgcg ggaaatggac ccggtggcga tgcgaccaat gctggaacgt
    38521 actgtcaagg gagcgttact gactaacctg ctgtttgttt tagggatatt cctaagccag
    38581 tgggcagcat aactgacaaa tatcaattaa caattgatga ttttgccaac agcccacata
    38641 gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt
    38701 ccgagctttg tgacatctat caagaagatg ttaacgtcgt ggaaccgctg ttctccaact
    38761 ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc gaggacaacg
    38821 ggttgctgta cgatctgctc gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg
    38881 gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg
    38941 aatgggaagg tctggtcatt tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg
    39001 atatcggcat ccaggcgatg gcggcaattc cggttggtgc cgctggcgaa ggcattggcg
    39061 aaagcgatgt ccgcgtcaat tttggcggtg tcaccttctt ctccggcgac catctttatg
    39121 ccgacaatac cgggattatt ctttcagaag atccgctgga tattgaatga taagaaaggc
    39181 accgcaaggt gccttttttc tgcgttacct gttggcctac acagtaaaga aattacgcga
    39241 aagatgaagc gtaatcagac ctcttccatg cgacccagca gggcctgcag acgttcctgc
    39301 cagccgttct gctgttcttt cagatggttg ttctcacgct ccagctcttc gcgctgatgc
    39361 tgggcatttt gaacttcctg cgacagtgag ttgttttttt ctttcagctc ttcgatttcc
    39421 atctgcaaca gagtgatggt atcaatcgcc tgctgtactt ttgcttccag tttatcaaac
    39481 acttctaatg acattgtcat acctctcctg aattgcaagg cgttgatgga taaaaaatcc
    39541 tcgtcccgat taccggtgac gccttaataa atacgagcgc actttagtta gctccgattg
    39601 tatgaagccg cgccatcgct gtccagcggc acgccttgca gattacggtt tgccacactt
    39661 ttcatccttc tcctagtgac ataatccaca tcaatcgaaa atgttaataa atttgttgcg
    39721 cgaatgatct aacaaacatg catcatgtac aatcagatgg aataaatggc gcgataacgc
    39781 tcattttatg acgaagcaca cacattttaa gttcgatatt tctcgttttt gctcgttaac
    39841 gataagttaa cagcatgcct acaagcatcg tggaggtccg tgactttcac gcatacaaca
    39901 aacattaact cttcaggatc cgattatgag tcaaacatca accttgaaag gccagtgcat
    39961 tgctgaattc ctcggtaccg ggttgttgat ttttttcggt gtgggttgcg ttgcagcact
    40021 aaaagtcgct ggtgcgtctt ttggtcagtg ggaaatcagt gtcatttggg gactgggggt
    40081 ggcaatggcc atctacctga ccgcaggggt ttccggcgcg catcttaatc ccgctgttac
    40141 cattgcattg tggctgtttg cctgtttcga caagcgcaaa gttattcctt ttatcgtttc
    40201 acaagttgcc ggcgctttct gcgctgcggc tttagtttac gggctttact acaatttatt
    40261 tttcgacttc gagcagactc atcacattgt tcgcggcagc gttgaaagtg ttgatctggc
    40321 tggcactttc tctacttacc ctaatcctca tatcaatttt gtgcaggctt tcgcagttga
    40381 gatggtgatt accgctattc tgatggggct tatcctggcg ttaacggacg atggcaacgg
    40441 tgtaccacgc ggccctttgg ctcccttgct gattggtcta ctgattgcgg tcattggcgc
    40501 atctatgggc ccattgacag gttttgccat gaacccagcg cgtgacttcg gtccgaaagt
    40561 ctttgcctag ctggcgggct ggggcaatgt cgcctttacc ggcggcagag acattcctta
    40621 cttcctggtg ccgcttttcg gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg
    40681 caaactgatt ggtcgccatt tgccttgcga tatctgtgtt gtggaagaaa aggaaaccac
    40741 aactccttca gaacaaaaag cttcgctgta atatgactac gggacaatta aacatgactg
    40801 aaaaaaatat atcgttgcgc tcgaccaggg caccaccagc tcccgcgcgg tcgtaatgga
    40861 tcacgatgcc aatatcatta gcgtgtcgca gcgcgaattt gagcaaatct acccaaaacc
    40921 aggttgggta gaacacgacc caatggaaat ctgggccacc caaagctcca cgctggtaga
    40981 agtgctggcg aaagccgata tcagttccga tcaaattgca gctatcggta ttacgaacca
    41041 gcgtgaaacc actattgtct gggaaaaaga aaccggcaag cctatctata acgccattgt
    41101 ctggcagtgc cgtcgtaccg cagaaatctg cgagcattta aaacgtgacg gtttagaaga
    41161 ttatatcagc agcaataccg gtctggtgat tgacccgtac ttttctggca ccaaagtgaa
    41221 gtggatcctc gaccatgtgg aaggctctcg cgagcgtgca cgtcgtggtg aattgctgtt
    41281 tggtacggtt gatacgtggc ttatctggaa aatgactcag ggccgtgtcc atgtgaccga
    41341 ttacaccaac gcctctcgta ccatgttgtt caacatccat accctggact gggacgacaa
    41401 aatgctggaa gtgctggata ttccgcgcga gatgctgcca gaagtgcgtc gttcttccga
    41461 agtatacggt cagactaaca ttggcggcaa aggcggcacg cgtattccaa tctccgggat
    41521 cgccggtgac cagcaggccg cgctgtttgg tcagttgtgc gtgaaagaag ggatggcgaa
    41581 gaacacctat ggcactggct gctttatgct gatgaacact ggcgagaaag cggtgaaatc
    41641 agaaaatggc ctgctgacca ccatcgcctg cggcccgact ggcgaagaga actatgcgtt
    41701 ggaaggtgcg gtgtttatgg caggcacatc cattcagtgg ctgcgcgatg agatgaagtt
    41761 gattaacgac gcctacgatt ccgaatattt cgccaccaaa gtgcaaaaca ccaatggtgt
    41821 gtatgtggtt ccggcattta ccgggctgag tgcgccgtac tgggacccgt atgcgcgcgg
    41881 ggcgattttc ggtctgactc gtggggtgaa cgctaaccac attatacgcg cgacgctgga
    41941 gtctattgct tatcagacgc gtgacgtgct ggaagcgatg caggccgact ctggtatccg
    42001 tctgcacgcc ctgcgcgtgg atggtggcgc agtagcaaac aatttcctga tgcagttcca
    42061 gtccgatatt ctcggcaccc gcgttgagcg cccggaagtg cgcgaagtca ccgcattggg
    42121 tgcggcctat ctcgcaggcc tggcggttgg cttctggcag aacctcgacg agctgcaaga
    42181 gaaagcggtg attgagcgcg agttccgtcc aggcatcgaa accactgagc gtaattaccg
    42241 ttacgcaggc tggaaaaaag cggtcaaacg cgcgatggcg tgggaagaac acgacgagta
    42301 atgtcaggca cctgaatcga ttgcctgatg ctgatcgcct gatgcgacgc ttacgcgtct
    42361 tatcaggcct acgagccaat gccattttgt gtaggcggga taaggcgttc acgccgcatc
    42421 cggcatcacg cagactctga aaccgtgcgg gggcaacccc gcacacatca ataatcccac
    42481 ccttcccctg tgctacactt cgcgccattc cttactgctt agagtttgct atgagacgag
    42541 aacttgccat cgaattttcc cgcgtcaccg aatctgcggc gctggctggc tacaaatggt
    42601 taggacgcgg cgataaaaac accgcggacg gcgcggcggt aaacgccatg cgtattatgc
    42661 tcaaccaggt caacattgac ggcaccatcg tcattggtga aggtgaaatc gacgaagcac
    42721 cgatgctcta cattggtgaa aaagtcggta ctggtcgcgg cgacgcggta gatattgctg
    42781 ttgatccgat tgaaggcacg cgcatgacgg cgatgggcca ggctaacgcg ctggcggtgc
    42841 tggcagtagg cgataaaggc tgcttcctca atgcgccgga tatgtatatg gagaagctga
    42901 ttgtcgggcc gggagccaaa ggcaccattg atctgaacct gccgctggcg gataacctgc
    42961 gcaatgtagc ggcggcgctc ggcaaaccgt tgagcgaact gacggtaacg attctggcta
    43021 aaccacgcca cgatgccgtt atcgctgaaa tgcagcaact cggcgtacgc gtatttgcta
    43081 ttccggacgg cgacgttgcg gcctcaattc tcacctgtat gccagacagc gaagttgacg
    43141 tgctgtacgg tattggtggc gcgccggaag gcgtggtttc tgcggcggtg atccgcgcat
    43201 tagatggcga catgaacggt cgtctgctgg cgcgtcatga cgtcaaaggc gacaacgaag
    43261 agaatcgtcg cattggcgag caggagctgg cacgctgcaa agcgatgggc atcgaagccg
    43321 gtaaagtatt gcgtctgggc gatatggcgc gcagcgataa cgtcatcttc tctgccaccg
    43381 gtattaccaa aggcgatctg ctggaaggta ttagccgcaa aggcaatatc gcgactaccg
    43441 aaacgctgct gatccgcggc aagtcacgca ccattcgccg cattcagtcc atccactatc
    43501 tggatcgcaa agacccggaa atgcaggtgc acattctctg agtgatttga tcgattgagc
    43561 cttccagtcc ttcgggactg gaattttttt gttcggagaa cgaagataag gcaagtcaat
    43621 caaaacagga gaaaaacatg gctgattggg taacaggcaa agtcactaaa gtgcagaact
    43681 ggaccgacgc cctgtttagt ctcaccgttc acgcccccgt gcttccgttt accgccgggc
    43741 aatttaccaa gcttggcctt gaaatcgacg gcgaacgcgt ccagcgcgcc tactcctatg
    43801 ttaactcgcc cgataatccc gatctggagt tttacctggt caccgtcccc gatggcaaat
    43861 taagcccacg attggcggca ctgaaaccgg gcgatgaagt gcaggtggtt agcgaagcag
    43921 ctggcttctt tgttctggat gaagtaccgg actgcgaaac gctatggatg ctggcaaccg
    43981 gtacagcgat tggcccttat ttatcgattc tgcaactagg caaagattta gatcgcttca
    44041 aaaatctggt cctggttcac gccgcacgtt atgccgccga cttaagctat ttgccactga
    44101 tgcaagaact ggaaaaacgc tacgaaggca aactgcgcat tcagacggtg gtcagtcggg
    44161 aaacggcagc ggggtcgctc accggacgga taccggcatt aattgaaagt ggggaactgg
    44221 aaagcgcgat tggcctgccg atgaataaag aaaccagcca tgtgatgctg tgcggcaatc
    44281 cacagatggt gcgcgataca caacagttgc tgaaagagac ccggcagatg acaaaacatt
    44341 tacgtcgccg accgggccat atgacagcgg agcattactg gtaagcggtt acttatcgat
    44401 aaacggcacg atgagcaaat ccgcactcat cttattgatc atcccgcgat atgccggcat
    44461 caaacggttg ataaatgagt gatgatgacc acagacaagg aggtcgcact gctctttttg
    44521 cataatttcc agcagtgttt ccggcatttc tccgcgttca atacgcagtt ttgtcttcgg
    44581 ccattgaata tttttcgtca gtttatacag cttgttatcc gacttattct tcaacaattg
    44641 aagaatatct tctgttgcag ggaagtagat acccggatac aactcgctta agccatcatc
    44701 aatatgaatt aacgtcaggt gagcgtcatt atgtctggcg agctccaggg ctttattcac
    44761 cagtaaggca tcttcttcat tcccggaaat tgccacgcca atgtgtttat aagccatgtt
    44821 taactccttc taaagcaact ccatcaagct aacgaacgca gtgataactc aaaaataatc
    44881 atctcggcct ggcatgagaa agtgaaggcc gcatcaagct caacttgccc ctccagatcc
    44941 ttaatcttat aggccacgct ggccccttca tctgcagaga atgcagcggc ggcaagctct
    45001 ttcagtttgg tcataaaaga ctctgcttct tcgcgggtgg caaaaaagcg cgaaaactta
    45061 ctggtacagt tgtcgttatc aatcaccgta ccgatatcaa tcgcacatcc tttagtactg
    45121 catttatcta cgacatcttt catagggcac ctctgtatat tcgcctctct gtcgattacg
    45181 acttaagaat aatgcgccgt taagggaaga acaaacaagt aatgatctta tggattacca
    45241 ttttttttga tattgcttaa ggtgaagagt tcagaaggat tttccgggta ttgtcatttg
    45301 catcgtggaa ataacaatcc cggaaaaaaa tagcagtaag agatatatta aaggttaatt
    45361 acttgattta ttgtcggctt tatacttcac atcctgagta tctttaccat atttattttc
    45421 gccttgtgtg ccaacaaacg caccaagatc gataagcatc atcaccagaa tcaacgtcgg
    45481 gacaaaacgc cccaccgccc attgccagac acccggtaaa atcgctcagt tacccgccag
    45541 cagcatccac gccacaatca tcagaaatgc ccatgcgccg gaacgcccgc gatcatgcaa
    45601 gcgcttaaca gttactgccg ctgttggcca gagcaagcac acaaggcaaa acgccgcggt
    45661 ctgaatatcg agtagattct tacccgccag tgagaaaagc accagcatgc ctgcgaacca
    45721 caggcctatc caaatccaga aatcacggcg tccaatacgc cctttaaatg agaataacca
    45781 ttgctgtatg gtcatgtaag ttccttgatg gttgtctttt ccaggattct acccttttga
    45841 caagggcgac agatatcgtt ttaatcggag ccagtcataa caaaaggtac tgtcaatgaa
    45901 gccagggtgt acgctgtttt ttctcttatg ttctgcatta accgttacaa caacggcgca
    45961 tgcacaaaca ccagatacgg caacgaccgc gccttatctg ctggctggag cccctacttt
    46021 cgatctctcc atcagccagt ttcgagaaga ctttaacagc cagaatccca gcctgccact
    46081 gaacgaattt cgtgccatcg acagcagtcc cgacaaagcc aatctcactc gtgctgcaag
    46141 taaaattaac gagaacttgt atgcttctac agcgctggag cgcggtacct taaaaatcaa
    46201 aagcattcaa atgacctggc tacccatcca ggggccagag caaaaagcag cgaaagcgaa
    46261 agctcaggaa tacatggcag cagtgatccg cacactcacc ccattaatga ccaaaacaca
    46321 aagccagaaa aaactgcagt cgctactaac ggcggggaaa aacaaacgtt attacaccga
    46381 gacagaaggt gcactgcgtt atgttgtcgc ggacaacggc gaaaaggggc tgaccttcgc
    46441 tgttgaaccg attaagctgg cgctatctga atcgcttgaa ggtttgaata aatgacaaaa
    46501 agcaaagcct ttgtgccgat gaatctctat actgtttcac agacctgctg ccctgcgggg
    46561 cggccatctt cctttattcg cttataagcg tggagaatta aaatgcgaca tcctttagtg
    46621 atgggtaact ggaaactgaa cggcagccgc cacatggttc acgagctggt ttctaacctg
    46681 cgtaaagagc tggcaggtgt tgctggctgt gcggttgcaa tcgcaccacc ggaaatgtac
    46741 atcgatatgg cgaagcgcga agctgaaggc agccacatca tgctgggtgc gcaaaacgtg
    46801 gacctgaacc tgtccggcgc attcaccggt gaaacttctg ccgctatgct gaaagacatc
    46861 ggcgctcagt acatcatcat cggccactct gaacgtcgta cttaccacaa agagtctgac
    46921 gaactgattg cgaaaaaatt cgcggtgctg aaagagcagg gtctgactcc ggttctgtgc
    46981 atcggtgaaa ccgaagctga aaacgaagcg ggaaaaactg aagaagtttg cgcacgtcag
    47041 atcgacgcgg tactgaaaac tcagggtgct gcggcattcg aaggtgcggt tatcgcttat
    47101 gaacctgtat gggcaatcgg tactggcaaa tctgcaactc cggctcaggc acaggctgtt
    47161 cacaaattca tccgtgacca catcgctaaa gttgacgcta acatcgctga acaagtgatc
    47221 attcagtacg gcggctctgt aaacgcgtct aacgctgcag aactgtttgc tcagccagac
    47281 atcgacggcg cgctggttgg cggtgcttct ctgaaagctg acgctttcgc agtgatcgtt
    47341 aaagctgcag aagcggctaa acaggcttaa gtctgacagg tgccggattt catatccggc
    47401 acttactttc cttaactcct cgccttaacg caaaatctca cactcatgat cctggatttc
    47461 ctcggctgaa gcacggttca gcgtcagtag atttcgttgt gtcgccagca acacaaacga
    47521 attatcactc tgccgtacca tcgccagccc gtagcttccc atatgttccc gcgcctcagg
    47581 cacttcttct gcaagcatca taaatgggct gcgttgtacc agttcgcttt ccgttacccg
    47641 acgcgccagg tattcatgcc cgcgcaaacc acctggcagt ggcaaccagc ggctgctgat
    47701 gttcgccaga tttttatcca gttgttcgcg cacatcagga cgaatacaag agatatgaat
    47761 atgaaaatgg ttttgcgtac gcccggtgcg ggagttgatc gccaaagaaa ccgcgcgatc
    47821 gggaaccggc tggccgtatt ttttgctcat aaaatcacgc gcctgccagg ccaaccaaaa
    47881 gaagttcggc gttgaaggat cggtcaacaa aggactttca gtaccgttaa tacgatacgt
    47941 tgacatcaac agatattgca gtggaccatt aagatctttt aaaaccacgt atccggcatt
    48001 gggtttgact tccgcacatg gcgaaggatt ttgattttgc tgctgattgg gcaaacattc
    48061 ctcaaggaca atcttacgta atgtatccga ctcttcaccg gttaatttcc agtaaccaat
    48121 accggcagcc acaacggcga taactatcat caccaaaaaa agaagacccg cttttttcat
    48181 ctttttttcc ctgtacctca aagagatgca agggtaacgc aaaatcgtga caaataaaaa
    48241 acccggtcgc gagaaaccac cgggtttgat aattatccta gggaaatcag cgtttgctga
    48301 tctgatcgaa cgtaccgccg ttagcaaaat gctctttttg cgctttcgtc cagccgccga
    48361 actcttcatc aatggtgaat aacttcagct ttggaaacgc attttcgtac tttttcgcta
    48421 cttcagcgtc gcgcggacgg tagtagtttt tcgcggcaat ttcctgacct tctggcgagt
    48481 agagatattt caggtaggct tccgccacct ctttggtgcc ttttttctcg accactttat
    48541 cgaccaccga caccgttggc tctgcgagga tagactcact cggcgtgacg atttcgaatt
    48601 tatctttccc cagttcattc gctgccagca gagcttcgtt ttcccaggcg atcagtacat
    48661 cgccaatccc gcgctcgaca aaggtgttag tggagccgcg cgcgccagaa tccagaactt
    48721 cgacgttttt atacagtgcc cgaacaaaat cctgtgcttt tgcctgatcg ttgttgttgt
    48781 gatgcagcgc gtagccccag gcagccaggt agttccagcg tgcgccacca gagcttttcg
    48841 gattaggcgt gatcaccgaa acacccggtt taatcagatc gttccagtca tggatctgct
    48901 tcggattgcc cttacgcacc aggaaaacaa tggtggaagt gtacggtgcg gagttatccg
    48961 gcagacgttt gatccactct ttatcaatcc gcccgcgttc cgcaatcgcg tccacgtcat
    49021 aggccagagc cagcgtgaca acatcagctt caataccgtt gattaccgac gtcgcttgtt
    49081 tacctgaacc accgtgtgac tgacgaatca ccacgttatc accagtttgc tgtttccagt
    49141 gggcgctgaa tgccttgttg tactgttcgt acaattcgcg cgttggatca tatgaaacgt
    49201 taagaagctg aatatccttt gccataacgc tggttgccgc cagcaaaaat gttaacccta
    49261 cgccccactt gttcatcgcc cgactctctt atgttgtgtt gtgatgagca aagcgtgcca
    49321 gaaggttaac caaacattaa agaataaaaa aagattggct ataacttgcg ggtatatgtt
    49381 gagggattaa aaaggcggaa gaaatccgcc tcatattgct gacaaagtgc gctttgtcca
    49441 tgccggatgc ggcgcgaacg ccttatccgg cctacaaaag tttgcaaatt caataaattg
    49501 cagaattcat gtaggcctga taagcgaagc gcatcaggca tttttgcttc tgtcatcggt
    49561 ttcaggctaa aggaatctgc ctttttccga aatcattaat acagtttttt cgcgcagtcc
    49621 agccagtcac ctttgaacgg acgcttcatg ttttcgatag cgtcgatgat gtcgtggtga
    49681 accagctgtt cgttctggat acctacacaa cgaccgccgt aacctgccag cagcagatcg
    49741 atagcgtaag cgcccatacg ggaagccaga atacggtcgt aaggcaccgg agaaccaccg
    49801 cgctggatgt ggcccagcac agttgcgcgg gtttcacgac cggtttcttt ctcgatgaaa
    49861 tgcgccagtt cgtcaacatc acacatatgt tcggtaatcg ccacgatcgc gtgtttttta
    49921 cctttcgcga tacccgcttt gatttcgttt accaggtctt cacggctgaa ttcaacttcc
    49981 ggaactacaa cgaattcaca gccaccggca atggccgcag ccagggtcag atcgccacaa
    50041 taacggccca tcacttccac cacggaaata cgctggtgag aagaagaggt gtcacgcaga
    50101 cggtcgatcg cttctacaac ggtgctcagc gcagtgaaga aaccgatagt gtagtcagtg
    50161 cctttgatgt cgttgtcgat agtgcccggc agaccgatgc acgggaagcc catttcggtc
    50221 agacgcattg cacccatgta ggaaccgtca ccgccgataa ccaccagcgc gtcgatacca
    50281 cgttttttca ggttttcgat agccacggcg cggatgttct cgtcgcggaa ttccgggaaa
    50341 cgcgcagaac cgaggaacgt accgccacgg ttgatcatgt cagacacgct gtaacggtct
    50401 agctgtacca tacggtcttc atacagaccc agatagccgt cataaatacc cattacttcc
    50461 agaccttctg tcagcgcaga acgaacaacc ccgcgaattg cggcgttcat gcctggcgca
    50521 tcaccgccgc ttgtcaacac acctattttc ttaatcatga ctacctctga actttggaat
    50581 gcaaaatgaa atctgttgcc ggaagtcttc ttgcacatcg aagtgatcca acgaatgtgc
    50641 aaatagtata acaatcactt cctgctgaat tgattcaggt caggccaaat ggcggtattt
    50701 tatacacaaa atgcgggtct ggctctcttt tatactgatt atgaaagcat agaccgttta
    50761 ccctccctgg gtacgacgga acaggggtcc tgatggataa tcacatccga tcccggaaaa
    50821 cgccgtaaaa tagcctgctc tacctgatcc gccaccatat gtgcctgaac caaaggcaga
    50881 gagtcttcca tttccaaatg aatctgaata aagcgggtcg gccctgactg ccgcgtgcga
    50941 agatcgtgag cgccgctaac acccggccag gaagtcacga tatcaataat ttcttgccgt
    51001 tcttcatcag gcaatgcgcg atccagtaat gactgtaccg cctcatatcc catgcgtaac
    51061 gcgctatata aaatatagat gccgattccc aatgcaaaca gagcatcggc gcgatgccag
    51121 ccgtaccaag acaaccccag cgccagcaga attgcgccgt tcatcataac atcagactgg
    51181 taatgtagca tatcagcccg caccgcctgg ctttgcgtcc ggcgcaccac ccaccgctga
    51241 aacgagacaa ggataatcgt acaaattagc gccacaattg tcacgataac cccgacgcct
    51301 ggatctgtca tcggtgttgg agataccaga tgttgaatac ccgtcaaaaa caggaatagt
    51361 gccgaaccgg agataaacat actttgcgcc agcgccgcga gggactcagc tttaccgtga
    51421 ccaaacgagt gattatcgtc ggcaggttgc agggaatatc gcaccaccag taaattcgtc
    51481 aacgacgcgc cgatatccac cagcgaatcc accagcgcgg cgagaatact caccgacccg
    51541 gtataccacc atgcaaaaat tttaatcagc aatagcaacg aagccatcgc cgtcgcagca
    51601 atcgccgccc gactgaccag ccgtccataa gattgattca taaatactcc cgctatcaac
    51661 tgacgctagt ataacggaag caaatcatct gcaatgcatt aagcagcagg caaattgagg
    51721 ataaaaaaaa ccccacatca tgtgggggaa gacagggatg gtgtctatgg caaggaaaac
    51781 agggtttact actgggaacg tgagttgcta ctactcaata gcttcaacga tgaacttttt
    51841 tgccattgcg tcacgtcacg caactgctcc attcgttgtt gatgtttctc gtttaaaacc
    51901 gcttgctgct ccggcgttaa caggcgatac atttggttgc ggacttttgc catctcaacc
    51961 tgacgagcaa tttgctcatt cgccattttt tctgcctgtg cgcgcacagc gttttcatca
    52021 aaattttctg cggtgacaag gcgatgcatt gtctccagtt cgctaacatt aacaggaggc
    52081 tgttcgtgcc gggcctgttg cataagatct cgcatctgct gacgctgatg ttcggttaaa
    52141 cttatgccgt cgaacatatg gctctgcgta ctgcgctgcg taagttcttc acccggatgc
    52201 cagttatcgc ctgaaccgac ttcagcagcg tggcttaatg aactgactgc cagcgttgag
    52261 gccatgacgg cagcggtaac tatgcgcatc atttgctccc aaaatctttc tgtcgcgatt
    52321 caacgataga gagtttacga ttcaggctgc aaacatgcgt cagggggtgt aaaacaacgt
    52381 aaagtcatgg attagcgacg tctgatgacg taatttctgc ctcggaggta tttaaacaat
    52441 gaataaaatc ctgttagttg atgatgaccg agagctgact tccctattaa aggagctgct
    52501 cgagatggaa ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc ttgatcttct
    52561 ggacgacagc attgatttac ttttgcttga cgtaatgatg ccgaagaaaa atggtatcga
    52621 cacattaaaa gcacttcgcc agacacacca gacgcctgtc attatgttga cggcgcgcgg
    52681 cagtgaactt gatcgcgttc tcggccttga gctgggcgca gatgactatc tcccgaaacc
    52741 gtttaatgat cgtgagctgg tggcacgtat tcgcgcgatc ctgcgccgtt cgcactggag
    52801 cgagcaacag caaaacaacg acaacggttc accgacactg gaagttgatg ccttagtgct
    52861 gaatccaggc cgtcaggaag ccagcttcga cgggcaaacg ctggagttaa ccggtactga
    52921 gtttaccctg ctctatttgc tggcacagca tctgggtcag gtggtttccc gtgaacattt
    52981 aagccaggaa gtgttgggca aacgcctgac gcctttcgac cgcgctattg atatgcacat
    53041 ttccaacctg cgtcgtaaac tgccggatcg taaagatggt cacccgtggt ttaaaacctt
    53101 gcgtggtcgc ggctatctga tggtttctgc ttcatgatag gcagcttaac cgcgcgcatc
    53161 ttcgccatct tctggctgac gctggcgctg gtgttgatgt tggttttgat gttacccaag
    53221 ctcgattcac gccagatgac caagcttctg gatagcgaac agcgtcaggg gctgatgatt
    53281 gagcagcatg tcgaagcgga gctggcgaac gatccgccca acgatttaat gtggtggcgg
    53341 cgtctgttcc gggcgattga taagtgggca ccgccaggac agcgtttgtt attggtgacc
    53401 accgaaggcc gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg taactttatt
    53461 ggtcaggccg ataacgccga tcatccgcag aagaaaaagt atggccgcgt ggaactggtc
    53521 ggtccgttct ccgtgcgtga tggcgaagat aattaccaac tttatctgat tcgtccggcc
    53581 agcagttctc aatccgattt cattaactta ctgtttgacc gcccgctatt actgctgatt
    53641 gtcaccatgt tggtcagtac gccgctgctg ttgtggttgg cctggagtct ggcaaaaccg
    53701 gcgcgtaagc tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg ccagcacccg
    53761 gaactggaag cggggccaca ggaattcctt gccgcaggtg ccagttttaa ccagatggtc
    53821 accgcgctgg agcgcatgat gacctctcag cagcgtctgc tttctgatat ctctcacgag
    53881 ctgcgcaccc cgctgacgcg tctgcaactg ggtacggcgt tactgcgccg tcgtagcggt
    53941 gaaagcaagg aactcgagcg tattgaaacc gaagcgcaac gtctggacag catgatcaac
    54001 gatctgttgg tgatgtcacg taatcagcaa aaaaacgcgc tggttagcga aaccatcaaa
    54061 gccaaccagt tgtggagtga agtgctggat aacgcggcgt tcgaagccga gcaaatgggc
    54121 aagtcgttga cagttaactt cccgcctggg ccgtggccgc tgtacggcaa tccgaacgcc
    54181 ctggaaagtg cgctggaaaa cattgttcgt aatgctctgc gttattccca tacgaagatt
    54241 gaagtgggct ttgcggtaga taaagacggt atcaccatta cggtggacga cgatggtcct
    54301 ggcgttagcc cggaagatcg cgaacagatt ttccgtccgt tctatcggac cgatgaagca
    54361 cgcgatcgtg aatctggcgg tacaggattg gggctggcga ttgttgaaac cgccattcag
    54421 cagcatcgtg gctgggtgaa ggcagaagac agcccgctgg gcggtttacg gctggtgatt
    54481 tggttgccgc tgtataagcg gagttaaact ccgcatttgt aggcaggata aggcgtttac
    54541 gccgcatccg gcatttgagc aggatgcctg atgcgacgct gatagcgtct tatcaggcct
    54601 acactccggg ggttattttc cccacaaccg gcgggaaaaa tcttcgatct tgccgctcag
    54661 tcggcgcttt tgcatcgtcc gcgtccagct tttcgataac ccggcggcgg agagtaaacg
    54721 gtgatactga tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct cctgcacggt
    54781 cacatcgctg acacgggaaa ccaactccag cggcgcgtcc gcagacactt tccctggcgc
    54841 tatcacgcta tacaaccacc ccaccttacc tgtgttttgc atcaactgcg ctatatcgct
    54901 gatatcaaaa tggtaattga gtttatagca aggcgagcgc ggctggctga cctgaattaa
    54961 tgcctctccc cagcggaaaa tatcgcccat atgaacatta ctttccgtga gaccgtcggt
    55021 tgagagattt tcgccaaacg caggcgcaac aaacaactcc gcctgttccg gaaattcccg
    55081 cacccagtag agatagtgct cacgaggata atgacacagc gctctgtctg gcccgccgtg
    55141 aacctttttc tccgcctgct cgtcaccttc cagccccagc tccgtcagca tcaactcacc
    55201 atcaacctgg attttggcaa ttgcgctggg tttgccttcg ggataagcct gaatcttgcc
    55261 tgtgtataca tcaaccggat atcgcatctg ttccctctcc acgagaaatt tccccattag
    55321 accatattta ttttatgtca gccgtgctgt tttcgcggta agtatccagg caggaactca
    55381 atagcaaacc ggacgaacgt tagttcatcc ggctgtgggg attaaggaag caggtttatt
    55441 tcaccgtacc taaaatttcg attttcgctg ctctggcttt gactttacgt gaccagatag
    55501 aggtaagaat tggcaccaga atcgaagtca caatgaccgc cgtcgctacc agcgaagttg
    55561 ctgccggagc catcggttta aacgcaggca ccatttctgc aatcagcaca ggtgtcgcta
    55621 ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt gccatcgcca ccgccaatca
    55681 atttatcggc gataatcaac ggaataccgg tcacgataat tattgccaca cccaacagga
    55741 tccccagcaa accagtctgg gcaatcacag tcaaatcaat ggtattgccc agcgcgaagg
    55801 caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa ttctcgtaat tcagggtcaa
    55861 gattccccag ggcaaagccc accaggaacg gtaatactgc gccgacgaaa acatgcggtt
    55921 caaacgaggc aatcccggca gtgcccagaa taatcatcgt catgagcggc ccggactcca
    55981 acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc gtactgctgc atgatggaag
    56041 cgtaaagtcc gccgttggtc atatccatcg ccgccaccag cgccagcgtt gaaagtccgg
    56101 caaagaatcc aacttcaaca ccatgttccg gaataatgcg cgaggcaatt gccgcaacca
    56161 cccacgcgac ggcaatttta gttaccacca gcgtaccgga tttacgcagt accgttcccg
    56221 tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca caccgccaga atgggcaccg
    56281 taccggtaat catcccgttg gtgaatgatc caaaatattt ccccgccccc ggcgagaagg
    56341 tgtggcacag tgcgccaagg aatagcggga cgagcatcat cccccccggg attttctcaa
    56401 tcgagcgttt tatctgcatt tccatcacct gtcggcatat ttttaagggc tgatgcgtaa
    56461 ttcctcaatg gagcatcagt gttgatggaa acgaagatag acattcattt aatcaataaa
    56521 agtgatcaaa accacatcaa ttgaaacgct gttttatttt tataggatca ttgtttttat
    56581 tttggaattg atcacaaaaa aaccaccgcc gttggcgatg gttcattttt taagctgata
    56641 tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt attttttcgc cgcaaaacgt
    56701 gccgctgctt cgtcccagtt caccacgttc cagaactctt tgatgtagtc cggacggcgg
    56761 ttctggaatt tcaggtagta agcatgttcc cacacatcca ggcccagaat cgggaagccg
    56821 gaagcgccag aaatagcttc acccatcagc ggagaatcct ggttagcagt agaaaccacc
    56881 gccagtttat cgcctttcag caccagccat gcccagccgg aaccaaagcg ggaagctgcc
    56941 gctttttcaa attctgcttt gaagttatca acggagccga agtcacgttc gatagccgct
    57001 ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt tccagaacag gctgtggtta
    57061 gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt ctgctggcag ctggtccagt
    57121 ttggtgatca gctcttcaac cggcaggttg gcaaattctg gcaggctttc cagcgccgcg
    57181 ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt ggatttccat ggtctgctta
    57241 tcgaagtgcg gttccagggc atcgtaagca tacggcaggg atggcagggt atagctcata
    57301 ttcatctcca gtattgtcgg gcggccgatt gttaatgccg cgtaagcagt tggtttatta
    57361 tagttaatta aatgatattg aaaatgatta tcaatgccgc acttttcgta agggtatggt
    57421 tttgcaggaa aatgcccgag atgtgaagca aatcacccac ttaatgccgt gattgccagt
    57481 aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca gcacgatttt tacactcatc
    57541 tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag aagatgagta acgcgattac
    57601 gatggggata ttttggcatt tgatcggcgc ggccagtgca gcctgttttt acgctccgtt
    57661 caaaaaagta aaaaaatggt catgggaaac catgtggtca gtcggtggga ttgtttcgtg
    57721 gattattctg ccgtgggcca tcagcgccct gttactaccg gatttctggg cgtattacag
    57781 ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc ggcgctatgt gggggatcgg
    57841 taatatcaac tatggcctga ccatgcgtta tctcggcatg tcgatgggaa ttggcatcgc
    57901 cattggcatt acgttgattg tcggtacgct gatgacgcca attatcaacg gcaatttcga
    57961 tgtgctgatt aacaccgaag gcggacgcat gacgttactc ggcgttctgg tggcgctgat
    58021 tggcgtaggg attgtaactc gagccgggca gttgaaagag cgcaagatgg gcattaaagc
    58081 cgaagagttc aatctgaaaa aagggctggt gctggcggtg atgtgcggca ttttctctgc
    58141 cgggatgtcc tttgcgatga acgccgcaaa accgatgcat gaagccgctg ccgcacttgg
    58201 cgtcgatcca ctgtatgtcg ctctgccaag ctatgttgtc atcatgggcg gcggcgcgat
    58261 cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg aaggatttgt cgctaaaagc
    58321 cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg ttactctcgg cactgggcgg
    58381 gttgatgtgg tatctgcaat tctttttcta tgcctagggc cacgcccgca ttccggcgca
    58441 gtatgactac atcagttgga tgctgcatat gagtttctat gtattgtgcg gcggtatcgt
    58501 cgggctggtg ctgaaagagt ggaacaatac aggacgccgt ccggtaacgg tgttgagcct
    58561 cggttgtgtg gtgattattg tcgccgctaa catcgtcggc atcggcatgg cgaattaatc
    58621 tttctgcgaa ttgagatgcg ccactggctg ggcgtcatcc cggtttcccg ggtaaacacc
    58681 accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact gattaacagg
    58741 cggctatgct ggagaagata ttgcgcatga cacactctga cctgccgcag atattgattg
    58801 atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact gcacgatgcc
    58861 tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag ccgggtaatc
    58921 agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt gtaacgatgg
    58981 cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt agcaaacggc
    59041 acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg cgccatcccc
    59101 acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg aatcgctccc
    59161 tgccagtcaa gattcagctt cagccgctcc gggcaataaa taatattctg caaaaccaga
    59221 tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tataaaagag atcgccacgg
    59281 gtaatgcgat aaggacgatc gttgagtaca tgcagaccat taccgcgcca gacaatcacc
    59341 agctcacaaa aatcatgtgt atgttcggca aagacatctt gcggataacg gtcagccaca
    59401 gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa ctgatgcgcc
    59461 accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg tacaaatacg
    59521 ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat cacgcggtga
    59581 ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga atccacagcg
    59641 ataggcgatg tcagtgacgc tggcctcgct gtggcgtagc agatgtcagg ctttcatcag
    59701 tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa gctgccgatg
    59761 tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca cctcatcggc
    59821 aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt tttccaggtt
    59881 ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga tctcgcgact
    59941 ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa ccagctgtcg
    60001 cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc catccagctc
    60061 ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg agcgatacag
    60121 cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat gatcgcgtac
    60181 gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat gaatacccgt
    60241 gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa aatccgcctg
    60301 cgggagccgg ggttctatcg ccacggacgc gttacaagac ggaaaaaaat ccacactatg
    60361 taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa tcacgaggtc
    60421 aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt tttcaagata
    60481 cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaattcag caaattgtga
    60541 acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc agtaacgaga
    60601 aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag gatcacatta
    60661 tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc cagtgggcgc gtgatgctgg
    60721 cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt tttaacaatg
    60781 ggctgcatag ccaaaacggt tatgtcacct gggatgtgga tagcctggaa agtgccattc
    60841 gccttggatt aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt gggattgata
    60901 cctggggcgt ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc ctgcccgttg
    60961 cttatcgcga tagccgcacc aatggcctga tgccgcaggc acaacagcaa ctcggcaaac
    61021 gcgatattta tcagcgtagc ggcatccagt ttctaccctt caatacgctt tatcagttgc
    61081 gtgcgctgac ggagcaacaa cctgaactta ttccacacat tgctcacgct ctgctgatgc
    61141 cggattactt cagttatcgc ctgaccggca agatgaactg ggaatatacc aatgccacca
    61201 ccacacaact ggtcaatatc aatagcgacg actgggacga gtcgctactg gcgtggagcg
    61261 gggccaacaa agcctggttt ggtcgcccga cgcatccggg taatgtcatt gggcactgga
    61321 tttgcccgca gggtaatgag attccggtgg tcgccgttgc cagccatgat accgccagcg
    61381 cggttatcgc ctcgccgtta aacggttcac gcgccgctta tctctcttct ggcacctggt
    61441 cattgatggg cttcgaaagc cagacgccat ttaccaatga cacggcgctg gcagccaaca
    61501 tcaccaatga aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt atgggcttat
    61561 ggctgcttca gcgagtgctt caggaacggc aaatcaacga tctcccggcg cttatcgccg
    61621 cgacacaggc acttccggcc tgccgcttca tcatcaatcc caatgacgat cgctttatta
    61681 atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg ggaaacggcg caaccgatcc
    61741 cgggaagtga tgctgaactg gcgcgctgta ttttcgacag tctggcgctg ctgtatgccg
    61801 atgtgttgca tgagctggcg cagctgcgcg gtgaagattt ctcgcaactg catattgtcg
    61861 gcggcggctg ccagaacacg ctgctcaacc agctatgcgc cgatgcctgc ggtattcggg
    61921 tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat cggcatcgag ttaattacgc
    61981 tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc gcgaatctga
    62041 ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag attcactcta
    62101 cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct gggagctggc
    62161 gaaacagcgt ttcgcggcgg tgaggattga tgtcgaggag gcgctgcgcc aacttgatcg
    62221 tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg aaaacccgga
    62281 aggttcgctg accgggggga ttcaggccac aggcaattat ccgggcaaag cgcgtaatgc
    62341 cagtgagcta cgtgccgatc tggaacaggc tatgcggctg attccggggc cgaaacggct
    62401 taatttacat gccatctatc tggaatcaga tacgccagtc tcgcgcgacc agatcaaacc
    62461 agagcacttc aaaaactggg ttgaatgggc gaaagccaat cagctcggtc tggattttaa
    62521 cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt acgctctcgc atcccgacga
    62581 cagcattcgc cagttctgga ttgatcactg caaagccagc cgtcgcgttt cggcctattt
    62641 tggcgagcaa ctcggcacac catcggtgat gaacatctgg atcccggatg gtatgaaaga
    62701 tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg atgaggtgat
    62761 cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt gagagcaaat tgtttggcat
    62821 tggcgcagag agctacacgg ttggctccaa tgagttttac atggggtatg ccaccagccg
    62881 ccagactgcg ctgtgcctgg acgccgggca cttccacccg actgaagtga tttccgacaa
    62941 gatttccgcc gccatgctgt atgtgccgca gttgctgctg cacgtcagcc gtccggttcg
    63001 ctgggacagc gatcacgtag tgctgctgga tgatgaaacc caggcaattg ccagtgagat
    63061 tgtgcgtcac gatctgtttg accgggtgca tatcggcctt gacttcttcg atgcctctat
    63121 caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc tgctgcgtgc
    63181 gttgctggaa cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt acactgcgcg
    63241 tctggcactg ctggaagagc agaaatcgtt gccgtggcag gcggtctggg aaatgtattg
    63301 ccaacgtcac gatacgccag caggtagcga atggctggag agcgtgcggg cttatgagaa
    63361 agcgattttg agccagcgtg ggtaaacact gccggatgcg gcacgagcgc cttatccggc
    63421 ctacgggtcg aatgtccatg cgggctgtca ccctcacccc ggccctctcc ctgaaagggc
    63481 gagggagaaa aacatagcct cgtgcggcaa cggagctgaa caccgctccg gacagtcccc
    63541 tcgcccctac ggggagaggg ttagggtgag gggaacaagc cgcaccatgt ctaaacaagg
    63601 caacctaatt tacaggaaca cagaacatgc aaaacattac ccagtcctgg tttgtccagg
    63661 gaatgatcaa agccaccacc gatgcctggc tgaaaggctg ggacgagcgc aacggcggca
    63721 acctgacgct gcgcctggat gacgccgata tcgcgcttta tcacgacaac ttccacccac
    63781 aaccgcgcta tattccgctc agccaaccca tgcctttact ggcaaatacg ccgtttattg
    63841 tcaccggctc cggcaaattc ttccgtaacg tccagctcga tcctgcagct aacttaggcg
    63901 tcgtaaaagt cgacagcgat ggcgcgggct accatattct ctgggggtta accaacgaag
    63961 ccgttccaac ttctgaactt ccggctcact ttctctccca ctgcgagcgc attaaagcca
    64021 ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc gccctcacct
    64081 atgtactgga aaacgacacc gcggtcttta ctcgccaact gtgggaaggc agcaccgaat
    64141 gtctggcagt attcccggat ggcgttggca ttctgccgtg gatggtgccc ggcacggacg
    64201 aaatcggcca ggcgaccgca caagagatgc aaaaacattc gctggtgttg tggcccttcc
    64261 acggtgtgtt cggttccgga ccgacgctgg atgaagcctt tggtttaatc gacaccgcag
    64321 aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa cagaccatca
    64381 gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt tacaccacta gccagtgcgc
    64441 tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggta aaccccgacg
    64501 cccacgaaga gtatcagcgt cggcataatc ccatctggcc ggaactggaa gcagtgttga
    64561 aatctcacgg tgtgcataac tacgccattt acctcgacaa agcgcgtaat ctgctgtttg
    64621 ccatggtaga gattgaatct gaagaacggt ggaatgcggt agccagtact gatgtttgcc
    64681 aacgttggtg gaaatatatg accgatgtta tgcccgctaa cccagataac agcccggtga
    64741 gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc cattgtgccg gagatgcctc
    64801 cggcacaata ttttaaattt cgatttgaaa aattaaacat attacatctc ccggtaagtt
    64861 atatttccct gatacattgt gagtaaatca caaaaataat gaataaccca ttaatgattc
    64921 atgtggttta tttaaataac cattatgtgc attactccgc aaatctgacc tttcactctg
    64981 tctcaaattg tcattgttta ccgacatatc gcctctcatc acttaatcgc tccaccatta
    65041 caattgatta aaatatattt tatggagtcc gattaatggc agctcttact gcaagctgta
    65101 ttgacctgaa tattcagggc aatggcgctt attccgttct ga
//
LOCUS       DAJTYG010000011        60669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTYG010000011 DAJTYG010000000
VERSION     DAJTYG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.011"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..320
                     /locus_tag="OO568_000760"
     CDS             <1..320
                     /locus_tag="OO568_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6640229.1"
                     /translation="VGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDS
                     NSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQV
                     TYP"
     gene            433..1227
                     /gene="panB"
                     /locus_tag="OO568_000761"
     CDS             433..1227
                     /gene="panB"
                     /locus_tag="OO568_000761"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6640230.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1239..2090
                     /gene="panC"
                     /locus_tag="OO568_000762"
     CDS             1239..2090
                     /gene="panC"
                     /locus_tag="OO568_000762"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6640231.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSTLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVGLA"
     gene            complement(2164..3066)
                     /gene="rpnC"
                     /locus_tag="OO568_000763"
     CDS             complement(2164..3066)
                     /gene="rpnC"
                     /locus_tag="OO568_000763"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6640232.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIPQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3340..3720
                     /gene="panD"
                     /locus_tag="OO568_000764"
     CDS             3340..3720
                     /gene="panD"
                     /locus_tag="OO568_000764"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6640233.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3724..4953)
                     /locus_tag="OO568_000765"
                     /pseudo
     CDS             complement(3724..4953)
                     /locus_tag="OO568_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(5017..5457)
                     /locus_tag="OO568_000766"
     CDS             complement(5017..5457)
                     /locus_tag="OO568_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6640234.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5562..6332)
                     /locus_tag="OO568_000767"
     CDS             complement(5562..6332)
                     /locus_tag="OO568_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6640235.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6329..7255)
                     /locus_tag="OO568_000768"
     CDS             complement(6329..7255)
                     /locus_tag="OO568_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6640236.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCLNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRQVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7364..8026
                     /gene="can"
                     /locus_tag="OO568_000769"
     CDS             7364..8026
                     /gene="can"
                     /locus_tag="OO568_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6640237.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="OO568_000770"
     CDS             complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="OO568_000770"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6640238.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8809..11199
                     /gene="gcd"
                     /locus_tag="OO568_000771"
     CDS             8809..11199
                     /gene="gcd"
                     /locus_tag="OO568_000771"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6640239.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="OO568_000772"
                     /pseudo
     CDS             complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="OO568_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
     gene            12961..13308
                     /locus_tag="OO568_000773"
     CDS             12961..13308
                     /locus_tag="OO568_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6640240.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13414..14280
                     /gene="speE"
                     /locus_tag="OO568_000774"
     CDS             13414..14280
                     /gene="speE"
                     /locus_tag="OO568_000774"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6640241.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQQS"
     gene            14296..15090
                     /gene="speD"
                     /locus_tag="OO568_000775"
     CDS             14296..15090
                     /gene="speD"
                     /locus_tag="OO568_000775"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6640242.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15128..15490)
                     /locus_tag="OO568_000776"
     CDS             complement(15128..15490)
                     /locus_tag="OO568_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6640243.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="OO568_000777"
     CDS             complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="OO568_000777"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6640244.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIINRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18618..20471
                     /locus_tag="OO568_000778"
     CDS             18618..20471
                     /locus_tag="OO568_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR6640245.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSTVRERLSERGARRLER"
     gene            complement(20542..21978)
                     /gene="lpdA"
                     /locus_tag="OO568_000779"
     CDS             complement(20542..21978)
                     /gene="lpdA"
                     /locus_tag="OO568_000779"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6640246.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPNPNPKAKKK"
     gene            complement(22303..24195)
                     /gene="aceF"
                     /locus_tag="OO568_000780"
     CDS             complement(22303..24195)
                     /gene="aceF"
                     /locus_tag="OO568_000780"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6640247.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24210..26873)
                     /gene="aceE"
                     /locus_tag="OO568_000781"
     CDS             complement(24210..26873)
                     /gene="aceE"
                     /locus_tag="OO568_000781"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6640248.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27034..27798)
                     /gene="pdhR"
                     /locus_tag="OO568_000782"
     CDS             complement(27034..27798)
                     /gene="pdhR"
                     /locus_tag="OO568_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6640249.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28339..29712
                     /gene="aroP"
                     /locus_tag="OO568_000783"
     CDS             28339..29712
                     /gene="aroP"
                     /locus_tag="OO568_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6640250.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29755..30609)
                     /gene="ampE"
                     /locus_tag="OO568_000784"
     CDS             complement(29755..30609)
                     /gene="ampE"
                     /locus_tag="OO568_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6640251.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30606..31157)
                     /gene="ampD"
                     /locus_tag="OO568_000785"
     CDS             complement(30606..31157)
                     /gene="ampD"
                     /locus_tag="OO568_000785"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6640252.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31245..32138
                     /gene="nadC"
                     /locus_tag="OO568_000786"
     CDS             31245..32138
                     /gene="nadC"
                     /locus_tag="OO568_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6640253.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32341..32781
                     /gene="ppdD"
                     /locus_tag="OO568_000787"
     CDS             32341..32781
                     /gene="ppdD"
                     /locus_tag="OO568_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6640254.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32791..34176
                     /gene="gspE"
                     /locus_tag="OO568_000788"
     CDS             32791..34176
                     /gene="gspE"
                     /locus_tag="OO568_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HCR6640255.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            34166..35368
                     /gene="hofC"
                     /locus_tag="OO568_000789"
     CDS             34166..35368
                     /gene="hofC"
                     /locus_tag="OO568_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR6640256.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALHYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35403..36446)
                     /gene="guaC"
                     /locus_tag="OO568_000790"
     CDS             complement(35403..36446)
                     /gene="guaC"
                     /locus_tag="OO568_000790"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6640257.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36608..36646)
                     /locus_tag="OO568_000791"
     CDS             complement(36608..36646)
                     /locus_tag="OO568_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640258.1"
                     /translation="MIIKTLKMSARK"
     gene            36671..37291
                     /gene="coaE"
                     /locus_tag="OO568_000792"
     CDS             36671..37291
                     /gene="coaE"
                     /locus_tag="OO568_000792"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6640259.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37291..38034
                     /gene="zapD"
                     /locus_tag="OO568_000793"
     CDS             37291..38034
                     /gene="zapD"
                     /locus_tag="OO568_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6640260.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38044..38241
                     /gene="yacG"
                     /locus_tag="OO568_000794"
     CDS             38044..38241
                     /gene="yacG"
                     /locus_tag="OO568_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6640261.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38341..38730)
                     /gene="mutT"
                     /locus_tag="OO568_000795"
     CDS             complement(38341..38730)
                     /gene="mutT"
                     /locus_tag="OO568_000795"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6640262.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38790..41495)
                     /gene="secA"
                     /locus_tag="OO568_000796"
     CDS             complement(38790..41495)
                     /gene="secA"
                     /locus_tag="OO568_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6640263.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41557..42069)
                     /gene="secM"
                     /locus_tag="OO568_000797"
     CDS             complement(41557..42069)
                     /gene="secM"
                     /locus_tag="OO568_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6640264.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42300..43217)
                     /gene="lpxC"
                     /locus_tag="OO568_000798"
     CDS             complement(42300..43217)
                     /gene="lpxC"
                     /locus_tag="OO568_000798"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6640265.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVCVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43318..44469)
                     /gene="ftsZ"
                     /locus_tag="OO568_000799"
     CDS             complement(43318..44469)
                     /gene="ftsZ"
                     /locus_tag="OO568_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6640266.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44530..45792)
                     /gene="ftsA"
                     /locus_tag="OO568_000800"
     CDS             complement(44530..45792)
                     /gene="ftsA"
                     /locus_tag="OO568_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6640267.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45789..46619)
                     /gene="ftsQ"
                     /locus_tag="OO568_000801"
     CDS             complement(45789..46619)
                     /gene="ftsQ"
                     /locus_tag="OO568_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6640268.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46621..47541)
                     /gene="ddlB"
                     /locus_tag="OO568_000802"
     CDS             complement(46621..47541)
                     /gene="ddlB"
                     /locus_tag="OO568_000802"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6640269.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLKASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47534..49009)
                     /gene="murC"
                     /locus_tag="OO568_000803"
     CDS             complement(47534..49009)
                     /gene="murC"
                     /locus_tag="OO568_000803"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6640270.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49063..50130)
                     /gene="murG"
                     /locus_tag="OO568_000804"
     CDS             complement(49063..50130)
                     /gene="murG"
                     /locus_tag="OO568_000804"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6640271.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50127..51371)
                     /gene="ftsW"
                     /locus_tag="OO568_000805"
     CDS             complement(50127..51371)
                     /gene="ftsW"
                     /locus_tag="OO568_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6640272.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51371..52687)
                     /gene="murD"
                     /locus_tag="OO568_000806"
     CDS             complement(51371..52687)
                     /gene="murD"
                     /locus_tag="OO568_000806"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6640273.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52690..53772)
                     /gene="mraY"
                     /locus_tag="OO568_000807"
     CDS             complement(52690..53772)
                     /gene="mraY"
                     /locus_tag="OO568_000807"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6640274.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53766..55124)
                     /gene="murF"
                     /locus_tag="OO568_000808"
     CDS             complement(53766..55124)
                     /gene="murF"
                     /locus_tag="OO568_000808"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6640275.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55121..56608)
                     /gene="murE"
                     /locus_tag="OO568_000809"
     CDS             complement(55121..56608)
                     /gene="murE"
                     /locus_tag="OO568_000809"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6640276.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56595..58361)
                     /gene="ftsI"
                     /locus_tag="OO568_000810"
     CDS             complement(56595..58361)
                     /gene="ftsI"
                     /locus_tag="OO568_000810"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6640277.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58377..58742)
                     /gene="ftsL"
                     /locus_tag="OO568_000811"
     CDS             complement(58377..58742)
                     /gene="ftsL"
                     /locus_tag="OO568_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6640278.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58739..59680)
                     /gene="rsmH"
                     /locus_tag="OO568_000812"
     CDS             complement(58739..59680)
                     /gene="rsmH"
                     /locus_tag="OO568_000812"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6640279.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59682..60140)
                     /gene="mraZ"
                     /locus_tag="OO568_000813"
     CDS             complement(59682..60140)
                     /gene="mraZ"
                     /locus_tag="OO568_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6640280.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCAIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14529 a        16807 c        14920 g        14413 t
ORIGIN      
        1 aggttggtac tgaaaacggg caactccttg gtaatactct cacaggtaat gacgcagcaa
       61 aaggagtcgg tgtactcata gaaggtttag caactagtaa aaatcctcta atgacattga
      121 aacctaatga ttcaaattct gtttataaag attacgaccc gagaggcaaa gacgatacaa
      181 caggaggagt ttacccggat caagatacag gtataacata ccctctccat ttccaggcca
      241 cgctacaaca ggatggaact ataccaatag aagctggtga gtttaaagcc accagtactt
      301 tccaggtaac ctacccttaa taagtccacc gcaccgccat catctggcgg tgcggtaatt
      361 gataaatcat tgcccccacc aatgtgactg ataaaatgcc aggcaacacg acatcattta
      421 tcaggatacg ttatgaaacc gaccaccatt gcttcacttc agaaatataa gcaggataaa
      481 aagcgtttcg cgaccatcac cgcttacgac tacagcttcg cgaaactatt tgctgatgaa
      541 gggcttaacg tcatgctggt aggcgactcg ctgggcatga ccgttcaggg ccacgattcc
      601 actctgccag taaccgttgc tgatatcgcc taccacactg ccgccgtacg tcgcggcgca
      661 ccaaactgcc tgctattggc tgacctgccg tttatggcgt atgccacgcc ggaacaagcc
      721 tttgaaaacg ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat tgagggcggt
      781 gagtggctgg tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt atgtggtcac
      841 ttaggtttaa ccccacagtc agtgaatatt ttcggtggct acaaagtaca ggggcgcggc
      901 aatgaagcga gcgatcgact actcagcgat gcattagcct tagaagccgc gggggcacag
      961 ctgctggtgc tggaatgcgt gccggttgag ctggcaaaac gtattaccga agcactggcg
     1021 atcccggtta ttggtattgg tgcaggcaac gtcactgacg ggcaaatcct cgtgatgcac
     1081 gacgcctttg gtattaccgg cggtcacatt cctaaattcg ctaaaaattt cctcgccgaa
     1141 acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc cggcgtttat
     1201 ccgggcgaag aacacagttt ccattaagga gtcacgttgt gttaattatc gaaaccctgc
     1261 cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg gcgctggtgc
     1321 ctactatggg taatctgcac aatggccata tgaagctggt cgacgaagcc aaagcccgcg
     1381 ccgatgtggt cgtcgtcagt attttcgtta acccgatgca gttcgaccgc ccggaagatc
     1441 tggcacgtta tccacggacc ttgcaggagg actgcgagaa gctaaacaaa cgtaaagtgg
     1501 atttagtttt cgccccttcg gtaaaagaga tctacccgaa cggtactgaa acccacactt
     1561 acgttgacgt tcctggcctt tcaaccatgc tggaaggtgc cagccgtccg ggacattttc
     1621 gcggcgtttc gactattgtc agcacgctgt tcaacctggt ccagccggac atcgcctgct
     1681 tcggtgaaaa agattttcag caactggcgt tgatccgcaa aatggttgcc gatatgggct
     1741 tcgatattga gattgtcggt gtgccaatta tgcgcgccaa agacggtctg gcgctaagtt
     1801 cccgtaacgg ttatctgacg gcggaacaac gcaaaattgc gcctggtctg tacaaagttt
     1861 taagttcgat tgctgacaaa ttgcaggctg gggaacggga tctcgatgaa attattacca
     1921 ttgcggggca agaactgaat gaaaaaggct tccgcgccga tgatattcag attcgcgatg
     1981 ccgacacatt gctggaagtt tctgaaacca gcaaacgggc agtaattctg gtagccgcct
     2041 ggcttggcga tgctcgcctg atcgacaaca aaatggtcgg gctggcgtaa tacttaactg
     2101 gcgctacggc tgatggcgcc agttattaat ttaccccacg tcatctgtca taacacaggt
     2161 aacttaaata aggttaatta ccttatcaat ctcagcaaga ggtaaatttg ccatctctgc
     2221 aacgtcttcc cgagacattc ctttacttag aagacgcagg gcgaattctt gcctttcctc
     2281 ctgtcttccc tgcggaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2341 catcatagac ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2401 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2461 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2521 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2581 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc
     2641 cggcgagtaa aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     2701 ataaaacagt atcggcacca ccagcggcag cttatcgtgg ccagcctcca gatgccggtg
     2761 catggcagca atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg
     2821 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     2881 gtccgtgctg tgacctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     2941 gttgagatca caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3001 cgtctccgca tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc
     3061 atccatcttc cctgtcttcc gtgtggcctt gcgtgaaggc agtatccagt caaggcgtgc
     3121 tatcgtcagc cacctcttta ttgtttaccg agcagcattc ataaaaatat caattcgtta
     3181 caggcgttac atggcacgct tcggcgcggt acgcaaactg ctgacgcaaa tgccatcaga
     3241 tgagtagaca ctcatcaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac
     3301 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3361 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3421 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat cgatatctgg
     3481 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3541 attatttctg ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc
     3601 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa tgtcgcctat
     3661 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     3721 ttgttatccc tgcggctggt tactcaccag ccgcgacatc ttctccagcg aatccgttct
     3781 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     3841 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     3901 gccgccaaac ggatacgaaa gataccagac atgtggatta aattgcgcca gagcgcggcg
     3961 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4021 ataaccatct acccgatgca aaaaatgggt atgtgactgg aaatcaaaaa catcgcgaat
     4081 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggctccact tctgcgggtg
     4141 acgtttgatg cgtgaggtaa caataaacac cgtcgccttc atgccatatt gtttcaacac
     4201 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4261 cgcagggaga ttgatcgtat tcttcacgta accttccagc tgagccatgc tcagtgtcgc
     4321 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4381 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4441 cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4501 acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4561 caaattgtcc gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tgtagaccgg
     4621 cgtatctttc taggtaatta agttctgatt actcagcggc ttgttaaggt cacccaaacc
     4681 gtcttcaact ttttgtcgcc cctgaactgg ctcgagatgc cctttatcga taaacccttt
     4741 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     4801 tccggcccga atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc
     4861 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     4921 catcagcagg agaataacag cttgtttata catgaaaaaa taaccgaggg tgaaacaacg
     4981 cttttattgt agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt
     5041 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5101 cagttcgaca atacactcac gaaactctga actggtcatg gtttcacggc aagccatcat
     5161 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5221 taataatgaa gccacgcgat acggtggcgc tccggctata tcggttaaga agatgacacc
     5281 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5341 taatccgcgc cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag
     5401 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5461 cttcctcctt agccgaaaat cagcctaccc tgaccattgt tgataaaggg tgatagccgt
     5521 caaaactcct ctcccccgaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5581 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     5641 ccaaaggtag tgaccagcgg cacatcattt ataccgagga agccgtagcg gaaaccactg
     5701 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     5761 agtgagtaaa agaccccgcc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     5821 ctgatgtcat cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaatagc
     5881 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     5941 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6001 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6061 ttacgctgga acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc
     6121 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6181 ccaatacgcg aacctatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6241 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6301 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6361 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6421 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgtagc acttcaactt
     6481 ccagcgtcgc agtatcgacc tgtcgatact gatagccatc gagcttcggt aacggacttt
     6541 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6601 aggtattttc caccagctca ccatgttgaa taatgccgat attgaggcac agcatttctg
     6661 cttcttccag gtagtgtgtg gtaagaatga tggtggtgcc tttgtcgttt aaatccttca
     6721 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     6781 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     6841 cagataacat acgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt
     6901 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     6961 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7021 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7081 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7141 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7201 gaacgccgcc tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7261 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7321 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7381 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7441 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7501 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7561 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7621 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     7681 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     7741 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     7801 gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc
     7861 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     7921 cttgctgcgt gatctggatg ttaccgccac caaccgcgaa acccttgagc aacgttaccg
     7981 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8041 atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt gccaatatac
     8101 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8161 atcgagaaac cgataaattc aaccgggacg ttcacttcac gacgggacgg tttatccagc
     8221 agcgtacaaa tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8281 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8341 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8401 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8461 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8521 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8581 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     8641 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     8701 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     8761 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     8821 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     8881 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     8941 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg
     9001 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9061 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9121 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9181 gtggtcgcac tgctgattag cggtggtatc ctgacctggg ccggatttaa cgatccgcag
     9241 gagatcaacg gcaccttaag cgccgatgcc acacctgccg aagctatctc ccccgttgcc
     9301 gatcaggact ggcctgcgta tggccgcaat caggaaggtc aacgcttttc accactgaag
     9361 caaattcacg ccgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat
     9421 gtgaagcagc cgaacgatcc gggtgaaatc accaatgaag tgacgccgat taaagtgggc
     9481 gatacccttt acctgtgtac cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc
     9541 aaagagaaat ggcattacga tcctgagctg aaaaccaacg agtctttcca gcacgtaacc
     9601 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
     9661 gattgcccgc gtcgtatcat tcttccagtg aatgatggtc gcctgattgc gattaacgct
     9721 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
     9781 atgccagaca ccaaaccggg tctgtatgag ccgacttctc cgccaattat caccgataaa
     9841 accatcgtga tggccggttc agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg
     9901 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa
     9961 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcacc aaactcgtgg
    10021 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10081 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10141 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10201 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10261 gtgccgatta tttacgctcc ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat
    10321 ggcgaactgg tggttccggc cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat
    10381 tacgttaccc ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc
    10441 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10501 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggtacgct ggtcttcccg
    10561 ggtaacctgg ggatgttcga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10621 attgccaacc caatggcact gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac
    10681 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    10741 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt
    10801 aaacagccag catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg
    10861 aagaaacgta ttggtacgcc gcaggacagt atgccgttcc cgatgccggt tccggtgccg
    10921 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    10981 atcgccgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11041 tggcagggtc gtttaccggc gggtggtcag gcaacaccga tgacctatga agtgaatggc
    11101 aagcagtacg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11161 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagaaatcc
    11221 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11281 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11341 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11401 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcgtg ccgtgaatat
    11461 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11521 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11581 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccatgc cccatatggc
    11641 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    11701 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    11761 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    11821 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg
    11881 ccatccccat ctggctgacc ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct
    11941 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12001 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12061 aattgagcga gcgggcatta cagccattga gcaaacgcag gcgcagccaa ccacgcgggg
    12121 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12181 cggcggtcat cacatccagt tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct
    12241 gaacgatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12301 cgtcatcttc aatcaccacc agaccagcca gccccatcgc cacctgtcgc cggtcttgcc
    12361 atgttgatgt ggatggaacc agcaggtagc ggcaggttga tcaacgttca acgtcaccga
    12421 gcgcttgcca cctggcggaa taattccctg tgggccgccg tcgacgtcac ccggtacttc
    12481 cagcccgtgc cagtgcaacg tcgtctcttc cgccagttgg ttgtagatat caaccgttac
    12541 cgctttgccg cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt
    12601 agttgcagtt ttctcgccaa aggtggactg acctgcgcca atagttaact gaatgcgatt
    12661 acgggcatcg gtcgtgagca aatcagggat cggtaacgtt gggcgttccg ccgcaaagac
    12721 tgcacggctc cacagtggca aggctgaagc cacacccagt gcgacggaat atttcaagaa
    12781 atcacgacgt tgcatagtta tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg
    12841 agcatagtcc ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat
    12901 ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca
    12961 atgaagacgt ttttcagaac agtgttattc ggcagcctga tcgccgtttg cgcaaacagt
    13021 tacgcgctca gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt
    13081 ctgaagaacg attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcactggtc
    13141 tttttcgccc agcaaaacca gtgggatctc agtaattacg acaccttcga catgaaagct
    13201 ctcggtgaag acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc
    13261 aaagccctgg cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct
    13321 ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt
    13381 ttttacgggt gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa
    13441 acgctacacg accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag
    13501 accgatcacc aggatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg
    13561 gatggcgtag tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat
    13621 gttccgctac tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt
    13681 gccatgctgc gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc
    13741 gatgcgggtg tcgtgtcgtt ctgccgtcag tatctgccca accataacgc cggtagctac
    13801 gacgatccgc gctttcaact agtgatcgac gatggcgtca atttcgttaa tcaaaccagc
    13861 cagacctttg atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagcctg
    13921 ttcacctcag cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc
    13981 gcacaaaacg gcgtctgctt tttacagcag gaagaagcca tcgacagcca tcgcaaactc
    14041 agccattact tcagcgacgt tggcttttat caggcggcga tcccgaccta ttacggcggt
    14101 atcatgactt ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt
    14161 attcaggcgc gttttctcgc ttctggcctg aaatgccgtt attacaatcc ggcaatccat
    14221 acggcagctt ttgccttacc tcagtatctg caagacgcac tggcttcaca gcagtcctaa
    14281 ggagaagata agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt
    14341 ctgagttttt gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat
    14401 attgcttata tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc
    14461 tgttccatta tcggggccaa tattcttaac atcgcccgcc aggattacga accacagggt
    14521 gccagcgtca ctattctggt gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca
    14581 gaacaccccg gcccactgcc agaaacggtg gttgctcatc tcgataaaag tcatatttgc
    14641 gtacatacct acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt
    14701 gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag
    14761 cttgagtccg atatcgtaac cattgattat cgcgtgcgcg gttttactcg cgacattaac
    14821 ggtatgaagc actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat
    14881 atgaaggcgc tgtatgacat ggtggatgta aacgtctatc aggaaaatat cttccatacc
    14941 aagatgttgc ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat
    15001 ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt
    15061 tattacgggc gcaatatgcc agctgtttaa cggctctggc ggagctccca ggctccgcca
    15121 gatttattta cttctgctgc acgaaattgc ggtaagccgc cacgacctgc agaaaatcct
    15181 caacgccgca tagcgacagg ctttcttcgt cgtagtagtt catcccctct tccatttcat
    15241 cgccagcgaa ttccagttga ttggcgcgaa ccatcacctc ttcagcgtcc atccacaggg
    15301 tgtattcatg ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt
    15361 gttccacttc atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga
    15421 ccacttcatg ccccatggac atacgcacct ttaccactcc ggtaatatcg cgcagaaatt
    15481 cgtaatccat agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat cgcgccattg
    15541 atgtaattat cgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa
    15601 aaatgcccgg gaatctcatt caccgggcat tggtgtcgtt tatgcgcagc gcgtgcgctt
    15661 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    15721 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    15781 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    15841 gcagaagcca ggaagacatt cgcgccagta cccaaacggt tcgggaagtt acgggtagag
    15901 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagggaacag
    15961 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc
    16021 agttgcgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg caactgacct
    16081 ttgtgcgcat ccagcagttt accagcggca cggaagtgac cgatgttagt catgcaagaa
    16141 ccgataaata cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc
    16201 ggatcgttcg gagcacacag gattggctct ttaatctccg ccagatcgat gtcgatcact
    16261 gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16321 atgccctgaa tacggcgttc cagggtacga cgatcgccgt aaccttccgc gatcatccac
    16381 ttcagcagga cgatgttgga gctcaggtat tcgatgatcg gttctttgtt cagcttgata
    16441 gtacaaccag cggcagaacg ctcggcggac gcatcggtta gctcaaaggc ctgctcaact
    16501 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16561 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcggaatggc gtgtaccaga
    16621 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    16681 atatccagcg gcattacgcc agttgcggca gcaaacgcca ccagaccaga acccgccggg
    16741 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    16801 agcagcatac ggttcagcca ggagtgaatg acgccgtcac ccggacgcag cgacacaccg
    16861 ccacggttaa taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    16921 tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    16981 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc
    17041 attttcggtt cgcagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc
    17101 attttctgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17161 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17221 atcagcggaa tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17281 aaggtcgcca gcagttcgcc tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17341 tcaatcacgt cgcccatgtt caggttagag acgttgactt cgattggcag tgcacccgcg
    17401 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17461 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17521 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17581 tgttgcagag cttcaatctg tttgatcggg ccaacaacac ctggctggtc tggctcaata
    17641 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac
    17701 gcatccggtg ccggagaaag gtcatcggtg ttggtttcgc cagtgacttt aaagacggta
    17761 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    17821 tgcattacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac gtcatagaag
    17881 ttatcgaaca tcagcagcgt gtgagaaagt gctttggcag caataggtgc cagtttggca
    17941 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt gcccagcagt
    18001 tcgatagctt tttccggagt cagcagaggg gatttagctt cgcctttagc gatagcagcc
    18061 aggaagcctg cttttacata ggcggcttca tcgacgcctg ggggaacacg gttggttaac
    18121 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg
    18181 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18241 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18301 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18361 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18421 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18481 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18541 cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt
    18601 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa
    18661 tttgcagtgc cgggctttgt gctgcctctg ccgggctata tataaaaagc cgcacagtgg
    18721 aagcgcctgt agaaccgcaa tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc
    18781 ttcctgcaac ggtttccgca cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca
    18841 ctgcacaaat agatcaatgg gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc
    18901 aggtgctgat ggcatcaacc tatccgacaa acgttgctca agcagtgcaa tggtcgcacg
    18961 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19021 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19081 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19141 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19201 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19261 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19321 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19381 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19441 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19501 acgcactatt cagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19561 atgatgatta tcatcaccac gatggcggtc atcgtgacag taatggctgg caacacaacg
    19621 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    19681 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    19741 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    19801 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    19861 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    19921 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    19981 tgacccagca gcaaaaggac gccgctcgtc agcgttatca gtcggcctct cctgagcagc
    20041 gccaggcagt ccgcgagaaa atgcagacta acccgcagat ccagcagcga agggaggcag
    20101 cgcgccagcg tattcagtcc gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc
    20161 agactaaccc gcagaaccag cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa
    20221 ctcccgaaca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aaccaacagc
    20281 aacgtgataa cgcccgccag cgtatacaat cggcatcacc tgaacaacgt caggtttttc
    20341 gggagaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat actgtcaggc
    20401 tgaataacga gcaacggtca acagtacgcg aacgtctctc tgaacgtgga gcaagaagac
    20461 tggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg
    20521 gatgttccgg caaacgaaaa attacttctt cttcgctttc gggttagggt tcgggttcgg
    20581 caggtcggta atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt
    20641 cgggtgcgcg tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag
    20701 cccgatttca cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacgcg
    20761 gtgagattct ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc
    20821 acgaccagaa gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc
    20881 ttctttctca gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat
    20941 aactttcgga tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc
    21001 gtgaacacct ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg
    21061 cggtacgttg gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac
    21121 gccagctttg cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac
    21181 ggcgtcgtaa cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc
    21241 gtcttctttc gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct
    21301 gatacgcttg gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa
    21361 catttcaacc acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc
    21421 gatgataccg ccacccatta ccagcaggcg ttctggcact tctttcagtt ccagcgcgtc
    21481 agtggagtcc cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga
    21541 acccgctgca atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac
    21601 ttccagggtg ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc
    21661 tttcgccata ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg
    21721 aatcttgtca atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt
    21781 ggcttcttcg ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt
    21841 caggcaaaca ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc
    21901 agcgcaacgg aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt
    21961 tttgatttca gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt
    22021 ctttcaggcc gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc
    22081 tcctggggat tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag
    22141 taatacccta accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa
    22201 tttttaaaca acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg
    22261 agattaccag aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg
    22321 aatgtcagac agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat
    22381 cacgcggtgg tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc
    22441 attccacacc ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt
    22501 cacaatcggc gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc
    22561 gccctgcatt tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag
    22621 ctcgcgagac agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag
    22681 accgttcggg gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg
    22741 accgtcttcc gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc
    22801 tttcatgatg aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc
    22861 gttctgctgt ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac
    22921 atgcgggatc atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc
    22981 cagttccact tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat
    23041 gccagggata ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt
    23101 cacgtaagcc tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt
    23161 cgccaggtta acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta
    23221 agcgtcgttt tcagcaaatt cagatttgcc ttccgctttc gcagccggag ctgctgccgg
    23281 agcttcagct tttgctgccg gagccggcgc tgccgcttcc tgtttcgcag gagctgccgc
    23341 aggcgctgcg ccttcaactt cgaagatcat aatcagcgaa ccagttttca ctttatcgcc
    23401 aacgttgact ttcagttcct tcacgacgcc tgcaaacgga gccggaactt ccatagaagc
    23461 tttatcgcct tctacggtaa tcagtgactg ttcagcggca actttgtcgc ccactttcac
    23521 catcacttcg gtcacttcaa cttcgtcacc gccgatatcc ggaacgttaa cttctttcac
    23581 gccagctgct ggtgcggggg tcgctgccgg agtcgcttcc tgtttagccg ccggagctgc
    23641 cgcgcctgct tcacccgcga cttcgaagac cataatcagc gagccggtag acactttgtc
    23701 acccacgttc actttgatct ctttcacggt acctgcaaac ggagccggaa cttccataga
    23761 agccttgtcg ccttctacgg tgatcagcga ctgttcagct tcaactttat cgccaacttt
    23821 caccaggatt tcggtcactt caacttcgtc gctgccgata tccggaacgt taacgtcttt
    23881 tgccgccgca gccgcgggtg ctgctgccgg agctgcttct ttcttctctt ctgcctgagc
    23941 aggtgcagcg tctgctgcac cgtcggcgga atcgaaaatc ataatcagtg cgccggtctg
    24001 ggttttatcg ccaacagaga ctttgatctc tttaacgata cccgcctgcg gagatggaac
    24061 ttccatagag gctttgtcgc cttctacggt gatcagcgac tgttcggctt caactttgtc
    24121 gcccactttg accaggatct cggtgatttc aacttcatca gccccgatgt ccggtacttt
    24181 gatttcgata gccattattc ttttacctct tacgccagac gcgggttaac tttatctgca
    24241 tcgatgttga atttggcgat tgcgtcagca accactttct tatcgatttc gccacgttta
    24301 gccagttcgc ccagcgccgc aaccacgaca taagaagcat caacttcgaa gtggtgacgc
    24361 aggttctcac ggctgtcgga acgaccgaag ccatcagtac ccagtacgcg atagtcgtca
    24421 gccggtacgt aagtacggac ctgctcagcg aacagtttca tatagtcggt agatgccact
    24481 gccggcgcgt cgttcatcac ctgagcgata tacggaacgc gcggagtttc cagcggatgc
    24541 agcatgttcc agcgttcaca atcctgacca tcacgcgcca gctcggtgaa ggaggtcacg
    24601 ctataaacgt cagaacctac gccgtaatct ttcgccagga tctctgccgc ttcacggacg
    24661 tgacgcagga tggaaccgga gcccagcagc tgaactttac ctttgctacc ttcaatggtt
    24721 tcgagtttgt agataccttt acggatacct tcctcagcac cttccggcat tgccggcatg
    24781 tggtagtttt cgttcagcgt agtgatgtag tagtaaacgt tctcttgttt ttcaccgtac
    24841 atacgctcca gaccgtcatg cataatgaca gcaacttcgt aagcgtaagc cgggtcgtaa
    24901 gagatacagt tcgggatagt cagcgactga atgtggctgt gaccatcttc gtgctgcaga
    24961 ccttcaccgt tcagggtggt acgaccggaa gtaccgccga tcaggaagcc acgcgcttgc
    25021 tggtctccag ccgcccagca cagatcgccg atacgctgga agccgaacat cgagtaatag
    25081 atgtagaatg ggatcatcgg cagattgttc gtgctgtaag aggtcgccgc tgccagccag
    25141 gaacaccctg cgcccagctc gttgatccct tcctgcagaa tctgaccttt ctcgtcttct
    25201 ttatagtaag caacctgctc gcggtcctgc ggggtgtact gctgaccgtt cgggctgtaa
    25261 ataccaatct gacggaacag accttccata ccgaaagtac gcgcttcgtc ggcgatgatc
    25321 ggtaccagac gatctttgat cgacttgttc ttcagcatca cgttcagagc acgaacaaaa
    25381 gcgatagtgg tagagagctc tttgctctgc tcttccagca gcgcgccgaa gtcttgcagg
    25441 ctcggcagct caagcttctc ggtgaagttc ggctgacggc ttggcagata accgtgcagt
    25501 ttctgacgct gagcgtgcag ataggtatgc tcttcagaac cttccgggaa ggtgatgtac
    25561 ggcagttttt cgatatctgc atcagacacc ggcacattga aacggtcgcg gatgtgacgc
    25621 acgccgtcca tgttcatttt cttaacctgg tgcgcgatgt ttttaccttc agccgcgtcg
    25681 cccatgccgt aacctttaat ggtatgagca aggattactg tcgctttgcc tttggtttcc
    25741 tgcgctttct tgaatgcagc gtagattttc ttcggatcgt gaccaccacg gttcagtgcc
    25801 cagatctgct cgtcagtcca gtctgcaacc agtgctgcgg tttcaggata tttaccgaag
    25861 aagtgttcac gaacgtacgc accatctttc gatttgaagg tctggtagtc gccgtcaacg
    25921 gtttcgttca tcagctggat cagtttaccg ctggtatctt tacgcagcag ttcatcccaa
    25981 cggctacccc acatcacttt gatcacgttc cagccagcac cttcgaagat gccttccagt
    26041 tcgttgatga tcttgccgtt accggtgacc gggccgtcaa gacgctgcag gttacagttg
    26101 ataacgaaaa ccaggttatc cagtttttca cgggtagcga tggtgatcgc acctttggat
    26161 tccggttcgt ccatttcacc gtcgccgagg aacgcgtaaa cggtctgttt agaggtatct
    26221 ttcaggccac ggtgttccag atatttcagg aatttagcct ggtaaatagc accaatcgga
    26281 cccagaccca tagatacggt cgggaactgc cagaattccg gcatcagttt cgggtgcgga
    26341 taggaagaga ggccattgcc gtgaacttcc tgacggaagt tatccagctg ctcctgagtc
    26401 agacgacctt ccaggaaagc acgggcgtaa acgcccgggg agatgtggcc ctggaagtaa
    26461 accaggtcgc cgccatcctg ctcgttgcgt gcacggaaga agtggttaaa gcacacatca
    26521 taaatggttg cggaagactg gaaggacgcc atgtggccgc ccagttcgag gtcttttttc
    26581 gacgcacgca gcaccgtcat gatggcgttc cagcggatag ctgaacgaat acggcgttcc
    26641 agttccagat tacccggata ctccggttgt tcttcaacgg ggatggtgtt gatgtagttg
    26701 ctgatacctg tgcctgcggc tacgtttaca ccgcctttgc gggcttcagc aagcagttgg
    26761 tcgatcagat actgagcacg ctcaacacct tcttcacgga tgaccgattc gatcgcctgg
    26821 agccagtcgc gagtttcgat cggatccacg tcatttggga aacgttctga catgggttat
    26881 tccttatcta tctaataacg ttgagttttc tggaacctgt cccattgaac tctcgccaga
    26941 aagctcaata agacaggttc tacgtttagt taccgcgctt ttatatgcgc ttgatttaca
    27001 acatcttctg gataattttt accagaaaaa tcactaattc tttcgttgct ccagacgacg
    27061 cagagaacgc tcacggcggc tctcttcacg gcttctgtcg agcaaaattt cttcgataaa
    27121 ggccagatgg cgatgcgatg cttcgcgcgc ttcttccggc ttaccggcca taatcgcttc
    27181 aaatatgcgg gtgcggtgac tactcaccag cggcagcatc tcgcgacgcg aatagagcaa
    27241 ttcgaagttc tggcggacat tctgggccaa catcggctcc atacacctta gcagatgaag
    27301 cagaaccaca ttgtgggccg cttcggtgac ggcaatctga tactggagta cggcgtttga
    27361 ttccgcgtcc agatcgccag actgctgcgc cagctctatg gcgtggtgga gttcacggat
    27421 gcgttccttg tcttcatcgg tactacgcag cgcggcgtaa taagcggcga taccttccag
    27481 ggcgtgtcgt gtttcgagca agtcatactg tgattcagga tggtcggaga gcagctccac
    27541 cagcggatcg ctgaagcttt gccataggct gctctggaca aaagtgccgc caccctggcg
    27601 acgaagcaac aagcccttcg cttcgagacg ttgaatcgcc tcacgcaagg agggacggga
    27661 gacgtcgaac tgttttgcca gttcgcgttc cggtgggagt ttttcgcccg ggcggagagt
    27721 gccttcgagg atcaaaaact ccagttgctg ctcaatcaca tcggagagtt ttggttggcg
    27781 gattttgctg taggccatga gttcctgtct taagccactt gccgaagtca attggtctta
    27841 ccaatttcat gtctgtgacg ctaaagtaac aaagtattca ccttatgtcc atacaggttt
    27901 tgattgaaat catgaaactg tgcacatttt aacaacttga catatataac gtttcaaagt
    27961 tgtaactatg cacaaatgta gactttacgt aggaaaggga ttttttaacg attattaaac
    28021 tataaaaatc cgaattgaac cgattcactt accaattttt tgatttttaa gtcaataaaa
    28081 acgaatttaa attcactctg catattgaga ggggttgagg ctgagcttta caaacggttt
    28141 ctttttaagc aactcatctt caaccatgca taaagcgggt gcattcgctg ccgcatacca
    28201 ttattcttga tctgacagaa gtctttttgt aacaattcaa acttccttga tgtaaataaa
    28261 tcaatacaac aaacggaatt gcaaacttac acacgcatca ctgcgtagat caaaaaaaca
    28321 atcaccgcac gaggtttcat gatggaaggt caacagcacg gcgagcagct aaagcgcggc
    28381 cttaaaaacc gccatattca gcttatcgcg ctgggtggcg cgatagggac agggctattc
    28441 ctgggtagcg cctccgtaat acagtccgca gggccaggga ttatcctggg ttacgccatt
    28501 gctggtttta tcgcctttct gatcatgcgt cagctgggtg aaatggtggt cgaagaacct
    28561 gtcgcaggct cctttagcca ctttgcttat aaatactggg gcagttttgc cggtttcgcc
    28621 tctggctgga actactgggt actgtacgtt ttagttgcca tggcagagct gactgccgtg
    28681 ggtaaataca ttcagttctg gtatccggaa atcccaacct gggtttctgc cgccgtattc
    28741 tttgtggtga ttaacgccat caacctgacc aacgttaaag tgtttggcga gatggagttc
    28801 tggtttgcca ttatcaaagt tattgcggta gtagcgatga tcatcttcgg cggctggctg
    28861 ctgttcagtg gcaacggcgg cccgcaggcg agcgttagca acctgtggga tcaaggtggt
    28921 ttcctgccgc acggcttcac cggactggtg atgatgatgg cgattatcat gttctcgttc
    28981 ggtggtctgg aactggtggg gatcaccgca gcagaagctg ataacccgga gcaaagtatc
    29041 ccgaaagcaa ctaaccaggt tatctaccgc atcctgattt tctatattgg ttcgttagcc
    29101 gttctgctct cactgatgcc gtggacccgc gttaccgccg ataccagtcc gtttgtgctg
    29161 atcttccacg agttaggcga tacctttgtg gcgaatgcgc tgaacatcgt ggtactgact
    29221 gcggcgctct ccgtgtacaa cagctgcgta tattgcaaca gccgtatgct gtttggtctg
    29281 gcacaacagg ggaacgcgcc aaaagcgctg gcgtctgtcg ataaacgcgg cgtaccggtt
    29341 aacaccattc tggtgtctgc gctggttaca gcattgtgcg tattgattaa ctatcttgct
    29401 ccggaatccg catttggcct gttaatggca ctggtggtat ccgcactggt gatcaactgg
    29461 gcgatgatca gtctggcgca tatgaagttc cgtcgcgcca agcaggaaca aggcgtggta
    29521 actcgcttcc ctgctctgct ttatccgctg ggtaactgga tctgcctgct gtttatggcg
    29581 gcggtactgg tgattatgct gatgacccca ggaatggcga tttcggtata cctgatcccg
    29641 gtatggctga tcgtgttagg tatcggctat ctgtttaaag agaaaaccgc caaagccgta
    29701 aaagcgcatt aatctctcta cgccctcacc cgtacagggt gagggcaata atctctacac
    29761 caacgccccg taaatcgtca gtagcgcaat caccaccacg accacgaacg aggttttctt
    29821 cgccattgaa accgctgcct tcggcgtctc caccttatcg acatgcggtt cacgcgccag
    29881 agagaactgc gccagacgcg ttaatacctg atattgcgaa gtatggaaat cacccagcga
    29941 agcaaaccag gccggtaacg ctttctcacc atgaccgatc aaggcatata ccacacccgc
    30001 aagacgaacc ggcacccaat ccagtacatg aagcactgca tcaatgccgg actgtaaacg
    30061 atgatgcggc gtctggtatc tcgccagcca gtattgccat gcacgcaaga acgcataccc
    30121 catcagcgta acgggtcccc aggttccccc cacaatcagc cagaacagcg gtgcaagata
    30181 aaaacgaaag ttaatccaca gcaatgcatt ttgcagctca cgcaaatact cacgttcgtc
    30241 gcagcctgcc gggacgccgt gaatcatggt gagttcgcca gccatcgtgg cacgggcatg
    30301 gctatcatta cgtgaagcag ctgtcagata agcatgataa tgaagacgaa ctttacctgc
    30361 gccaatacac agcaaaccaa tcagcagcca caccagcagc gtgggaacgt tgaacaatac
    30421 tccctgcaat gcgcgtaaca gtaaaaaagt cacacccatc gcaataatgg tcatgcctaa
    30481 cgtgcgcccg agagaaaaat gtttcacccg ccgaaagaac gcttcaagac gatgatcaag
    30541 ctgccagtgc tcgcccaact taaacaggcg ctcgaaaatt aacaccagta aggttgtaaa
    30601 tagcgtcatg ttgtctcctt gctgaccagc gcacgaaacc gtgcccaatc aaatgcagga
    30661 ccgggatcgg ttttccgatc cggcgcaata tcacaatggc ccgtcatgtt attagcgata
    30721 tcgggatagc gatctatcag tgcccgcgta accgccgcaa gctgttgata ctgcgcatcg
    30781 gtatacgcca gagtatcggt gccttcaagc tcaatcccaa tagaaaaatc attgcagcgt
    30841 tcacgcccct gatactgaga gactcccgca tgccaggcac gtttatcgaa aggaacatac
    30901 tggactattt caccatcacg gcgaatcaaa cagtgagcgg agacgcgcaa atgggcgatc
    30961 tcagcaaaga aaggatgtgc ctgcggatca atagttccag tgaataatgc gtcgatccac
    31021 ggaccgccaa actcgcctgg cggcaggcta atattgtgca ccaccagcag ggtgggtgtt
    31081 tcgtcatcca ggcggcaatc gtaatgtggt gagggaacgc ggcgcgcgcc aaccagccac
    31141 ccctgttcta acaacatgca ggatctcctt atatgtggtg ctaatacccg gttcagagta
    31201 gcatgtttct accttatgat tcgttagcta tctggagttt taacatgccg cctcgccgct
    31261 acaaccctga cacccgacgt gacgagctgc tggaacgcat taatctcgat atccccggcg
    31321 cggtggccca ggcgctgcgg gaagatttag gcggaacagt cgatgccaac aatgacatta
    31381 cggcaaaact tttaccggaa aattctcgct ctcatgccac ggtgatcacc cgcgagaatg
    31441 gcgtattttg tggcaaacgc tgggttgaag aggtgtttat tcaactggca ggcgacgatg
    31501 tcaccataat ctggcatgtg gatgacggcg atgtcatcaa cgccaatcaa cccttgttcg
    31561 aacttgaagg cccatcccgc gtgctgttaa cgggcgaacg cactgcgctt aattttgtgc
    31621 aaaccctttc aggagttgcc agtaaggtac gccactatgt cgaattactg gaaggcacca
    31681 acacgcagtt gttggatacg cgcaaaacct tacctggcct gcgttcagct ctgaaatacg
    31741 cggtactttg cggcggcgga gcgaatcacc gtctggggct ttctgatgcc ttcctgatca
    31801 aagaaaacca tattattgcc tccggctcag tgcgccaggc ggtcgaaaaa gcgtcctggc
    31861 tgcatccgga tgcgccagta gaagtcgaag tagagaatct ggaagaactt gatgaggccc
    31921 tgaaagccgg agccgatatc atcatgctgg ataacttcga aacagaacag atgcgcgaag
    31981 ccgtcaaacg caccaacggc aaggcgctac tggaagtgtc tggcaacgtc actgacaaaa
    32041 cactgcgcga atttgccgaa acgggcgtgg actttatctc cgtcggcgcg ctaactaaac
    32101 acgtacaagc actcgacctt tcaatgcgtt ttcgctaatt gctgacagat ttatccgctc
    32161 catcgcgggc ggataatctc cccttcccca actttcttcg taacgcctcg caaatttctt
    32221 gtctttcgct gcaacgaaat aaaaaacctc ggaatctgct ttcccgtttc gttttttgcc
    32281 cctcgcctgt gctgatggcg tttgccaaag tagcaccaac caaatcaagg agcgaaacag
    32341 atggacaagc aacgcggttt tacacttatc gaactgatgg tggttattgg catcattgcc
    32401 attttaagcg ccattggtat tcccgcttat caaaactacc tgcgcaaagc cgcactcacc
    32461 gacatgctac aaacctttgt gccttaccgt accgccgtag agttgtgcgc gctggaacat
    32521 ggtggattag atacctgcga cggtggcagc aatggcattc cctcgcctac caccacccgc
    32581 tatgtttcag ccatgagtgt ggcaaagggc gtggtgtcgc tgaccgggca agaaagtctc
    32641 aatgggctaa gcgtcgtcat gacgccaggt tgggataacg caaacggcgt caccggctgg
    32701 gcgcgcaact gcaatattca aagtgacagc gcattgcagc aagcctgcga agatgtcttc
    32761 cgctttgatg acgccaacta aggagcggca atgaatattc cacagctcac ggccctgtgc
    32821 ctgcgttatc agggagtctt gctggatgcc agcgaagaag tggttcatgt tgcggtggtc
    32881 gatgccccct cacatgagtt gctggacgca ttgcatttcg ctaccaccaa acgtattgag
    32941 atcacctgct ggacgcgcca acaaatggaa ggtcacgcca gtcgcacaca acagacattg
    33001 cccgtagctg ttcaggagaa gcatcagccc aaagcagagt tgctgactcg aacgttacaa
    33061 tctgcgctgg aacaacgcgc gtctgatatt catatcgaac cagcggacaa tgcctaccgc
    33121 atccgcttgc gtatcgacgg cgtattgcat cctttaccgg atgtttcacc ggatgccgga
    33181 gtcgcattaa cagccagatt aaaagtgctg ggaaacctgg atattgcgga acatcgcctg
    33241 ccgcaggacg ggcaattcac tgtcgaactg gcaggaaaca ccgtctcatt tcgtattgcg
    33301 accttaccat gtcggggtgg tgaaaaggtg gtattaaggt tgttacagca ggtgagccaa
    33361 gcactggatg ttaacacgct gggaatgcag ccgttacaac tggcggactt tgctcatgcc
    33421 ttgcaacaac cacagggact ggtgctggta actggcccta caggcagcgg caaaacggtc
    33481 acgctttata gtgccctgca aacgctgaat accgctgaca ttaatatttg tagcgtcgaa
    33541 gatccggttg agatccccat agccggacta aaccagacgc aaatccatcc gcgtgccggg
    33601 ctcacctttc agggtgtttt gcgtgcgtta ttgcgccagg atcctgacgt catcatgatc
    33661 ggagagatcc gcgatggcga aacggcagaa attgccatta aagccgcgca aaccggtcac
    33721 ctggtgttgt ccacgctaca caccaattcc accactgaaa cgctggtacg tttacagcaa
    33781 atgggggtcg cccgctggat gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg
    33841 gtacgtaaac tttgcccaca ttgtcgccga cagcaagggg agcccatcca tattccagac
    33901 actgtatggc cgtcgccgct gccccactgg caggcacccg gttgtgtaca ttgctaccac
    33961 ggtttttatg gtcgtaccgc cttgtttgaa gtcctgccca ttacaccagc catacgccag
    34021 cttatttccg ctaatactga agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg
    34081 cgtacgcttt ttgaaaacgg ctgcctggcc gtggagcaag gcttaaccac ctttgaagag
    34141 ttaatccgcg tactggggat gccgcatggc aagtaagcaa ctctggcgct ggcatggcat
    34201 aaccggcgac ggcaatgcgc aagatgggat gctatgggca gagagccgta ctttactgct
    34261 tatggcacta cagcaacaga tggttacccc actaagcctg aagcgaatcg ccatcaattc
    34321 tgcgcagtgg cgaggagata aaagcgcgga agtcattcat caactggcga cgctacttaa
    34381 agccgggtta acgctttctg aagggctggc tctgctggcg gaacagcatc ccagtaaaca
    34441 atggcaagcg ttgctgcaat cgctggcgca cgatctcgaa cagggcattg ctttttccaa
    34501 tgccttatta ccctggtcag aggcatttcc gccgctctat caggcgatga tccgcacggg
    34561 tgaactgacc ggtaagctgg atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca
    34621 gcgtcagttg accgacaaag tgaaatcagc gttgcattat cccatcatca ttttagcgat
    34681 ggcaatcatg gtggttgtgg caatgctgca ttttgttctg ccggagtttg ccgctatcta
    34741 taagaccttc aacaccccac taccggcact aacgcagggg atcatgacgc tggcagactt
    34801 tagtggcgaa tggggctggc tgctggtgtt gttcggcttt ctgctggcga tagccaataa
    34861 gttgctgatg cgccgcccga cctggcttat cgcgcggcag aaattgctgt tacgcatccc
    34921 gattatgggt tcactgatga ggggacaaaa actcacgcag atctttacga ttctggcgct
    34981 gacacaaagt gcaggcatta cttttttaca gggcgtagag agcgtcagag aaacaatgcg
    35041 ctgcccgtac tgggtgcaac ttctgacaca aatccagcac gatatcagta acggtcatcc
    35101 catctggctg gcgctaaaaa atgccggtga gtttagcccg ctctgtttgc aattagtgag
    35161 aacaggagag gcatccggct cgctggacct catgttagac aacctcgccc atcatcatcg
    35221 ggataacaca atggcgctgg cggataacct cgcagcctta ctggaaccgg cgttgctgat
    35281 cataacggga ggaattatcg gtacgctggt ggtggcaatg tatctgccaa ttttccattt
    35341 aggcgatgcg atgagtggga tgggataacg ctggcgtgtt gctccacgcc agcgttggga
    35401 gattacaggt tgttgaagat gcggttttct tgttccagca cacgaataaa cgtggtgcgc
    35461 ttggtcagct ctttcaggcg tgaagcccca acgtatgtac aagctgaacg caggccgccc
    35521 aaaatatcac gcgcggtatt ttcaaccggg cctcgcagcg gcagcttgac ggttttacct
    35581 tctgctgcgc ggtattccgc aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc
    35641 atgccgtaga acagcataaa tttctcgccg ttctcctcaa cgatgcgacc gccgctctct
    35701 tcgtggcccg ccagcatgcc gccaagcatg acgaaatcgg caccgccgcc aaaggctttc
    35761 gccacatcgc ccggcgtggt acagccaccg tcgctgacga tcattccgcc cagaccgtgc
    35821 gccgcatcgg cacattcgat taccgcagaa agctgcggat aaccgacgcc tgttttgacg
    35881 cgagttgtac aaacagaacc tgggccaatg ccaactttaa cgatatcagc accggagagg
    35941 ataagctcct cacacatttc accagtcact acgttgccag cacaaatggt tttggtcggc
    36001 cacgcttcac gcgctttcgc aacgaactgc acgaagtgtt cggaataacc attcgccaca
    36061 tcaatacaaa cgaagtttaa tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa
    36121 tccgcatcag acgtgccggt agaaaccatc acatgtttca gcacatcagc ggaagaattg
    36181 ttgataaacg cttgccactc ttcgacagaa aagtgtttat gcacagcagt cagaatatca
    36241 aaagaagcca gcgcagaggc catagaaaat gtgcctacgg tgtccatatt tgcggcgata
    36301 atcggcacgc cggaccagct ctgacctgaa tgtttgaagg tgaattgacg ttccagttca
    36361 acatcggaac ggcttttaag agtggagcgt ttagggcgga tgagaacgtc tttaaaacct
    36421 aacttcagat cttcttcaat acgcatgtgc ggattcctgg ggttaatggc tataagggta
    36481 agagcgcaac tccagtgacg ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa
    36541 ttgtcttttt ttacgctaca atcccataaa tttacctggt aaatagtgag cgaacgtttc
    36601 atcaatttca ctttcgcgcg ctcattttta acgttttgat aatcatgatg aattcccggg
    36661 aatataaatt atggggtata tagttgcctt aacgggaggc attggcagtg gtaagagtac
    36721 cgttgccaat gcgtttgctg atctcggaat taacgtcatt gatgccgata ttattgcgcg
    36781 tcaggtggtt gaaccaggtg cacctgcgct acatgccatt gctgatcact ttggcgaaaa
    36841 catgattgct gctgatggaa cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa
    36901 cccggaagag aaaaactggc ttaacgacct gctgcatccg ctgattcagc aagagacgca
    36961 acaccagatc cagcaagcaa cttcccccta tgtactgtgg gttgtgccat tgctggtaga
    37021 aaactcactg tataaaaaag cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca
    37081 acttaagcgc accatgcagc gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc
    37141 tgctcaggca acgcgcgaag cccgccttgc cgtggcagat gacgtcattg ataataacgg
    37201 cgcaccggat gctatcgcat cggatgttgc ccgcctgcac gcacactatt tgcagcttgc
    37261 gtcgcagttt gtctcacagg aaaaaccgta atgcagaccc aggtcctttt tgaacatcca
    37321 ctaaatgaaa aaatgcgtac atggctgcgc attgagtttt tgattcagca actcaccgtt
    37381 aatttaccca tcgttgacca cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta
    37441 ctggatgttt tcgagcgcgg cgaagtccgc actgagctgt tgaaagaact taaccggcag
    37501 caacgtaaac tccagacctg gattggcgtg cctggcgtgg accagagccg tattgaagca
    37561 ttaattcagc agttaaaagc ggcggggagc gtattaattt ccgcgccgcg tatcgggcaa
    37621 tttctgcgtg aagatcgttt gattgctctg gtgcgtcagc gactgagcat cccaggcggc
    37681 tgttgcagct ttgatttacc tacattgcac atttggctgc atctacccca ggcgcagcgc
    37741 gacagccagg tagaaacctg gattgccagc ctgaacccgc tcacccaggc acttaccatg
    37801 gtgctggatt taattcgcca gtctgccccc ttccgtaaac aaaccagcct gaatggtttt
    37861 tatcaggata acggtggcga tgccgacttg ctgcgcctga atctgtcgct cgattcacag
    37921 ctttatccgc aaatttccgg acataagagc cgttttgcca ttcgttttat gccgctggac
    37981 actgaaaacg gacaggtacc ggaacgtctg gatttcgaac tggcctgttg ctaaggagta
    38041 aaaatgtcag aaactattac ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt
    38101 gaaatcagcc cgtttcggcc attttgctcc aaacgttgtc agctgatcga cctcggagaa
    38161 tgggctgctg aagaaaaacg aatccccagc agtggcgatc tttccgaaag cgatgactgg
    38221 agcgaagagc caaagcagtg acatttgtct gatgcctcac gtaggcctga taagacgcgg
    38281 acagcgtcgc atcaggcatc ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccaac
    38341 ctacagacgc ttaagcttcg caattaccgg ttcattggct ggcggaaaat catcggcatt
    38401 aagaccgacc agcgacatcc actcaccggg ttgcccttct ttaccccacg gcaccccttc
    38461 ccaactttcg accagccaga accacagagt tatatgcctg tccgggaatt catattccag
    38521 tttttcaaat agcgaaaaat gttgtggggt aatcccgacc tcttcctgaa gttcacgcac
    38581 caccgcctgt tccggcgttt cgcccatttc aattttaccg ccgggaaact ccagtttatt
    38641 cgccatgtgc gcatctgctg cgcgacgtgt tataaagatt tcattgttct cgttgcgaat
    38701 aatacctacc gcaatttgca gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat
    38761 cctgcgcctt ttacttcaac tgttagcttt tattgcaggc gaccatggca ctgcttgtat
    38821 tttttaccag aaccgcacgg gcaaggatcg ttacgtccta ctttgcgttc accggtttgc
    38881 gccgccagtg cagctgcggc tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt
    38941 gctaaacgct cggcttccat acgacgctgt tgttccagct cctcaacctc ttcaggcata
    39001 cgtacctgaa ctttgctcag cgtactgata acttcatatt tcaacgactc cagcatcgct
    39061 gcaaacatgg agaacgattc acgtttgtat tcctgcttcg gatctttctg tgcatagcca
    39121 cgcaggtgga taccctgacg cagatagtcc atcgctgcca ggtgctcttt ccacagggag
    39181 tcaagcgttt gcagcatgac gcctttctcg aagtgacgca tcatctcagc accaaccact
    39241 tcttctttac gctgatacac ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct
    39301 tcatgcagtt ctggttcttt atccagccac tcggcaattg gtaaatcgag gtcgaaatcg
    39361 ttcttcagac gttcctgcag ccccggaata tcccacattt cttccagcga ctgtggcgga
    39421 atgtaggcat caatggtggc tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc
    39481 acatcgctga catccagcag ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta
    39541 gccacgtcat catattccag cagttgctta cgaatgtcga agttacgact ttcaacttta
    39601 cgctgggcgt tggcaatcgc tttagtcacc cacgggtgtt caatggcttc gcctggcttc
    39661 atacccagtt tacgcatcat gccggatact cggtcggaag caaaaatacg catcagcgca
    39721 tcttccatcg acaggtagaa acgggaagaa ccagcatccc cctgacgacc agaacgaccg
    39781 cgcaactggt tatcgatacg acgggattcg tgacgctcgg taccgatgat atgcaggcca
    39841 cctgcttcca gtaccgcatc gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc
    39901 tctgcggtcg gattttccag cgcggcaact tctgcctgcc agctacctcc gagcacaata
    39961 tctgtaccac gacccgccat gttggtcgcg atagtcaccg cagccggata acctgcctga
    40021 gcaacaatcg ccgcttcgtt ggcgtggaat ttggcgttca ggacattgtg cttaataccg
    40081 gctttggtca gttcgtttga caccagctcc gatttttcga tggagatagt acccaccagc
    40141 accggctggc ctttcgcagt acgttctttg atatcttcaa tgatcgcctg aattttttcc
    40201 gcttcagtca tgtagaccag gtccggcaga tctttacgaa tcattggacg gttggtcgga
    40261 acaacgacgg tatccagttt gtagatggag ctaaattcga aagcttcggt atcagcagta
    40321 ccggtcatcc ccgccagttt ttcatacaga cggaagtagt tctggaaggt gatcgaagcc
    40381 agcgtttggt tttcgttctg gatctgcaca ccttctttcg cttccacagc ctggtgcaga
    40441 ccatcggacc agcgtcggcc ctgcatggta cgaccggtgt gttcgtcaac gatgataact
    40501 tcaccatctt taacgatgta gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc
    40561 gccgttacgt ggtgcatcag catgatgttg gccggagagt acagagactc cccttcatcc
    40621 atgatgccct ctttcaccag cagttcttca atcagcacca gaccacgttc ggtcaggttc
    40681 acctggcgag atttttcgtc caccgagaag tggccttcgc cctggaaggt ttcggagtct
    40741 tctttttcct gacggatcag gtgcggaata attttattca cgcgtttata catttccgag
    40801 ctgtcttctg ccgggccgga aatgatcagc ggtgtacgcg cttcatcgat caggatggag
    40861 tccacttcgt ccaccagcgc atagtgcagt ttacgctgta cacgttcttc agggctgaac
    40921 gccatgttgt cgcgcaggta gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca
    40981 gctgcgtaag cttcgcgctt tgccggtgct ggcatgcccg gcaggttgat accaacagtc
    41041 aggccaagga attcaaacag cggacggttg ttttcggcgt cacgttgcgc caggtagtcg
    41101 ttgacggtaa ctacgtgcac gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt
    41161 gcggtcaggg ttttaccttc accggtacgc atttcggcga tgcagcgttc gttaagaacc
    41221 ataccgccga gtaactgaac gtcgaagtga cgcataccaa agacgcgctt acttgcctca
    41281 cgcaccacgg cgaaagcttc cgggatcaga ttttccagca cttcgccttt ttccagacgc
    41341 gcacgaaact ctgcggtttt ccctttcagt tcttcatcgg agagtttttc catctccggt
    41401 tccatggcat tgatgatgtt gaccactttg cgcatccggc gcagggtgcg atcgttacga
    41461 ctaccgaaaa ctttagttaa caatttgatt agcataataa aatctcaaac gccccgcgtt
    41521 acggagttaa taaaatgaag taaaggttta ttgttgttag gtgaggcgtt gagggccagc
    41581 acggatgcct tgcgcctggc ttatccagac gggcgtgctg aattttgctt gtggggtaaa
    41641 atgcgcataa tcaatgcgat aacccttttc agacggcgtg ccttcctggg tcagcagcgc
    41701 gctgagcgta tccagtaatg caagatgttg cgcctgaaga ggcaaagatt cttcagcaac
    41761 gggcagtgtt tgcggtgcca ttgcgaaaga aagatgacgg attaccgtgc gaatagcatg
    41821 ttgatgccag taatcaacgg aatagttcga attcgggcgg cgcgtgttcg cttccagcaa
    41881 ggccaattga ccaaagttaa ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc
    41941 gggcgcgttt ggttcggcgg cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat
    42001 ccctaataag agatgcggcc agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat
    42061 tccactcacg ttattgccat cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc
    42121 attaaagcct gaagtccaca gcacgaatga agataagttg tgcgtaaaag tgcttaagaa
    42181 aacagcgttc gcaccgtttt ttatagctct tataaaattc aaaaaaaaac gactggttca
    42241 cctggccgga gagagtgcca gatttgccag tcgaatttta tacgacagta taaatgtcgt
    42301 tatgccagta cagctgaagg cgctttgaag gccaacggca gttctgcgtc gtcctggaag
    42361 gtcacatatt cccaggcttc ctgtttcgcc aggacagcct gcagcagttt gttattcagt
    42421 gcatgaccgg atttataagc ggtaaatgca ccaataatat tgtgaccaca catgaacaag
    42481 tcaccgatcg catcgagcat tttgtgacgc acaaattcgt cttcaaaacg caggccgtct
    42541 tcgttcagta cgcgataatc gtcaacaacg atggcacaat cgaagctgcc gcccaggcac
    42601 aaaccacggg actgcagata ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg
    42661 atctggcgca taaacgcatc agcggagaag ttcatcgcat agcgctggtt gctggaatca
    42721 atagccggat ggttaaaatc gatggtgaaa tccagcgaaa aaccattgta cggcttaaat
    42781 tcagcccact tatcgccatc ttcgacacaa acagtctctt tgatgcgaac aaattttttg
    42841 gcgcagttca actcgtcgat accggcgtca agcagcaggt atacaaacgg agcggcgctg
    42901 ccgtccatga tcgggatttc cggcgcgtta acttcgataa caatgttatc gatgcccagg
    42961 cccgcgagag cagcattgag gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc
    43021 agacacgtac agagcatggt atcacgcaca gatttggcat cggccgggaa atctaccggt
    43081 ggattcaagt cggtgcgacg atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc
    43141 agggtgactt tcttgccggt atgtaaaccg acacccgtcg cctgaacgat acgtttaagt
    43201 gtcctttgtt tgatcatcgt attatctcgc caaattacct atccaaccga agtgtactat
    43261 acattcggcg ggccagttta gcacaaagag cctcgaaacc caaattccag tcaattctta
    43321 atcagcttgc ttacgtagga atgctgggat atccagataa tccggctctt tcgcagtttg
    43381 cggcgcattg tcattcacta ctttagcaac cggcttctgc tcctgggtca gcggagccat
    43441 cccatgctgc tggtagcgat ccatcactgg ctgctgaacc tgcttattgg tcaccagagt
    43501 gatttcagga cgtttgtcca tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc
    43561 attcatatcc gggtcaagag aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc
    43621 acggatggtg ttacctaccg tttcgaactc atccagacgc aggtcgaagc ccgccgtgat
    43681 gttaaccagc acgccgcgcg cgccagacag gtcgatatct tccagcagcg gagaagagat
    43741 agccatttca gcagcttctt ccgcacggtc ttcaccgctc gccacgccag aacccatcat
    43801 tgcgtagccc atttcagaca ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc
    43861 cggacgagta atcagttcgg cgataccttg cacagcgcct ttcagtacat cgttcgctgc
    43921 gccaaacgca tccagcaggg agataccgcg gcccagaact ttcagcagtt tgtcgttcgg
    43981 gatagtgatc agagagtcca catgcttgga cagttcagtg atcccctgct cggcgaatgc
    44041 catacgcttc ttgccttcaa agttgaaagg cttagtgacg acagcaacgg tcaggatacc
    44101 caaatctttt gccacttcag cgacgactgg tgctgcacct gtaccggtac caccacccat
    44161 acccgcagca ataaagacca tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc
    44221 ctcatcagcc gcattgcggc caacttctgg attagcgcca gcgcccagtc ctttggtgat
    44281 accgctaccg atttgaatcg tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt
    44341 atttaccgcg aagaattcaa caccttcaat acgctcgcgc accatgtgtt caacagcatt
    44401 accgccgccg ccgccgacgc cgatgacttt aatcaccgcg tcattggtaa gttccattgg
    44461 ttcaaacata gtttctctcc gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc
    44521 tcataaaaat taaaactctt ttcgcagcca actattgagt cgcttgatcc acgagccaac
    44581 tgatgctgta acacgttttt ctacttcagc ttcaccgtta agatgtgact ctttcccata
    44641 gtgaagcaat cccaccgccg tcgaataata cggctcctga gcataatccg ttaaaccggt
    44701 aatgttcagc ggcgcgccga tacgcacttg cgtatgaaac acgcgctgag cacaggctgc
    44761 aagaccttcg atctgcgctg cgccaccggt taatacaatg cctgccgcca ggtgatgttt
    44821 aaccccttgt tggcgaagct tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag
    44881 caactcggta tagcgcggct cgatcacctc tgccagggtc tgacgttgca gactccgcgg
    44941 cggacgaccg cctacgctcg gcacttccac gctctcatct tttccaacga tggaacccag
    45001 cgcacaaccg tggcgaactt taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta
    45061 agcgatatca ctggtcacga cattgccagc ataaggaatt accttagtgt ggcgcaatgc
    45121 cccaccggta taaacggcga tatccattgt accaccaccg atatcgacga cgcagacacc
    45181 cagttcacgt tcatcttccg tcaataccga ataacttgat gccagtccgg caaatatcag
    45241 ttggtcaact ttcagcccac aacgttcaac cgctttgacg atgtttttcg ccatatcgtt
    45301 gtgacatgtg atcaggtgca cttttgcctg catccgcaca cccgaaagtc ctaccggatt
    45361 cttgatccct tcctgatagt caatcgcata ctcttgcggg atcacatgca gcacacgatg
    45421 ctcatcgcgc acacgcaccg atttcgcggt atggacgacg ttttccacat cttcttgcgt
    45481 cacttcttct tcagaaatag gcaccatacc aatttcattc tggcagctga tgtgcttacc
    45541 agaaagcgcc agatataccg aagagatctg acaatctgcc atcaattctg cctggtcaat
    45601 ggcgcgttgt acgcacttga ccacggattc gaggtcgttc accccgcctt tatccatacc
    45661 acgtgacggg cagctgccca cgccaatgat attgaccata ccgtcgggca gaacttcccc
    45721 tactaaagcg gcaaccttcg cggtaccaat ttccagtcct actaccagtt ttctgtccgt
    45781 cgccttgatc attgttgttc tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc
    45841 ggcaagggcg cccagcctac tgccgctcca gagtcataac gcaaatcaac gtagctaatc
    45901 cgtttgccat cggtttgcgc ctgctgctgt aaaaccggat aaagttctac aaagcgagcc
    45961 aaacgtttca tcgtatcgcc ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac
    46021 tgccaggaac gccgcgcggt catcgccgct tccttcagag taaatctgtc ctttgccagc
    46081 atctgcccca tttcgcgata gccctgcaac acttcattag cgctgccttc cgggccatac
    46141 agcattggaa gcacctgctt gctggtgcga tctggcggca cgctgaaggt gtttccttcc
    46201 gcgtctacca tgtgttgatc attccaccgc gcaatcggca catattcaac cagatgaatc
    46261 ttcaattcat caggccactg ctttctgacg ctcacctgct taatccacgg caggcgttgt
    46321 tctatttgcg tctggatgat gttgacatcc tgggtcataa aggtacccgg ctcacccaat
    46381 gccaggatcg actgccggat atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc
    46441 agctttgaga gcggcaggcg ttgcgcatct tccatccagc tcaacacgac ccagccgctc
    46501 accaacactg tcgttaaaac ggtcagcagg aaaaggatcc ccgccagacg cgttccatta
    46561 ttgcggcgag aagaaacctc ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata
    46621 ttagtccgcc agttccagaa ttcgtactac caactgcgag aagctcatac ctgcctgacg
    46681 tgccgccatc ggcaccaggc tgtggctggt catacccggt gaggtattgg cttccagcag
    46741 ataaaactgt ccatcactgt ccagcataac gtcaatacgt ccccatcctt tgcaacctaa
    46801 cgtcgtccat gctttcagca ctaatgcctg caaattggcc tcttgtgacg ctttcagacc
    46861 tgcggggcag aaatactgtg tctcatcaga gagatacttc gcctcataat catagaaggt
    46921 tccggacggt tgaatacgta ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa
    46981 ctccggccca cttagccatt tttcaatcaa tacttcttca tcgtgctgaa atgccaatct
    47041 taatgcatct tgtagagcat tttctgctac tacttttgac attcccacac tggaaccttc
    47101 gcggctcggc ttaacgataa ccggcaaacc cagagcagaa atttctgcta actgcttatc
    47161 gctcaggcct ttttcaaact ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc
    47221 ggcaccttgc cacagaagtt tgctgcgtag tttatctatt gaaagcgcag atgccatcac
    47281 tccgctgccg gtataaggca agcccatcag ctcgagcatg ccctgcagcg taccatcttc
    47341 accgccgcga ccgtgtagcg cgataaacac tttctgaaaa cccatcgact tcagttgcgt
    47401 cacgtcgact tctttcgggt cgacaggata cgcatcaata ccgccttcac gcagcccggc
    47461 taacaccgct gcgccagaat tcagagaaac ttcccgctca gcggaagtcc cgcccaacag
    47521 gaccgcgatt ttatcagtca tgttgttctt cctccggagt ttgcggcttc agtttgattt
    47581 cagctaaaga acgggcaatt tttccgatat taccagcccc ctgaacgaga atcaggtcgt
    47641 taccggttaa taccggtgcc agcatctcgg ctacctgcgc agggtccggc accagaatgg
    47701 gatcaatttt cccacgtcca cgaattgtgc gacacagcga acggctgtcc gctcccggaa
    47761 ttggcgcttc gccagccgga tacacttcca gcatcaacag ggtatcaacc tgcgtcagca
    47821 cattggcgaa atcatcatac aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca
    47881 ttaccaggtt tttatccggc cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg
    47941 tcgggtggtg gccgtagtca tcgaccagca ttgccgtacc gcttttacca ttcactggct
    48001 ccagcgggaa ttcaccgagg aaatcaaaac ggcgaccagt cccctggaag ctctccagcg
    48061 cacgcaaaat agcctcgtcg tcaattccct cttccgtagc aaccgcaacc gcagctgcgg
    48121 cgttcagcgc gttatgacgg cctggcgcat tcagggtgac gcgcatcggc tctttgtcct
    48181 gacgcagcag cgtaaagtgc ccctgcgggc caatctgctg ataatcttct acacgcacgt
    48241 cggcatcttc gctgaagccg taagttgtgg tctgacgtcc cacacgcggt aacaattcgc
    48301 ggatcaccgg atcatcaaca cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa
    48361 aattaataaa ggtctgtttt aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg
    48421 cttcgatatt ggtgacaatc gccaccatcg gttgcagatg caggaacgat gcatcactct
    48481 catctgcttc ggcaatcagg taccgaccat gccccaaacg cgcatgaacc cccgccgctt
    48541 ttaccagccc gccgttaacg aaggttgggt cgagccccgc ttctgcgtag atgctggaaa
    48601 ccatcgcggt agttgtcgtt ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac
    48661 gcattaactc agccagcatt tcggcacgac ggatcaccgg aatacgcgct tcatgagctg
    48721 cgacaatttc cgggttgtcg gcagaaatgg cgctggaaac aacgaccacg ctggcatcac
    48781 gtacgttttc cgggcgatgg ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg
    48841 tgaccggatt tggcgctaaa tcggaaccac tgatctgata accttcattg gccagaactt
    48901 cggcaatacc gcccataccg gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac
    48961 gcatttcggg cacgatggaa cgcagttttg ccaattgttg tgtattcatt ctttacgcca
    49021 ttaacttctt aaattcatac gatgcaaaag gcatcgctac aattacgccc gggcagcccg
    49081 gctcacttca tttgccactc gctcggtggc atccggaatg gatgcagcgc gggcgcgttc
    49141 tgccatggtt aataaggttt ctcgcgacca cccggccagg gtgttggcga cagcatccac
    49201 gctaagctgt ggctgctcga taattttggc tgcgcccgct ttttccagcg gtagcgcatt
    49261 ccagtattgc tggcggtctt tatgttgaaa cggcacaaac aacgccggta gtcctgccgc
    49321 ggcgatttca ctcacagtta aagcaccgga gcggcaaacg acgacatccg cccacgcata
    49381 cgccgccgcc atatcatcaa taaattcagt cactttatgc tgcggttgcc ccgcttcggc
    49441 atacgcctgt tcaacggatt gttgcgaacc tttgccgctc tgatgccaga tagtgactga
    49501 atcacccagt ttcgcagcaa cctgcggcat tgtctggtta agaatgcgtg cgccctggga
    49561 accacccact accaacacac gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa
    49621 cggcagcgcc agcacatcgg tacgcaccgg gttacccacc acttccgcat tagggaaagc
    49681 gcctggaaac gcctgcatca ctttggtagc aatcttcgcc agccatttat tggttaagcc
    49741 cgcaataccg ttttgttcat gaagtacaac cggaatgcct aacgaccacg cggccagacc
    49801 acctggacct gatacatagc cgcccatacc gagtaccacg tcaggtttgt acgctttcat
    49861 aatcgcccgc gcctgacgcc aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt
    49921 tatacctttt ccacgcaaac cagagatacg aatgaaatca atttcgatgc catgttttgg
    49981 cactaagtcc gcttccatac ggtcggcagt ccccagccag cgaacttgcc aaccctgagc
    50041 cattagatag tgcgcaaccg ccagtcccgg gaatacatgt ccaccggttc cgcctgccat
    50101 caccattaat cgctttcctt gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc
    50161 tccagacgcg tttcataatc aatacgcaac agcatcatga tggctgtcga cataatcagt
    50221 aagctcgaac caccgtaact gattagcggc aatgtcagac ctttggtcgg taacatcccc
    50281 gccgccgcgc ctacgttaac cagcgcctgg aagctaaacc agatgccaat agaacaggcg
    50341 agaaaaccgg aaaaacggtg gtcaatttct aatgctttgc ggccaatcga catcgcacga
    50401 aaagcgacga agaataccat taaaagtgct agtaccacac cgacataacc taattcttcg
    50461 ccgataatgg cgaaaataaa gtcagtgtgc gcttccggca gatactccag tttttgtacc
    50521 gaattaccta aaccttgccc ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt
    50581 aactgatagc cgctgccaaa gggatcttcc cacgggttcc agaatgcggt aacacggcgg
    50641 atacggtacg gttcggcgag gatcagcaac acaaccgctg aaatgcccat accgataatg
    50701 gcaatgaact gccacaattt cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac
    50761 aacaccacca ccgtaccaag gtctggctgt gccagcagta acactgccaa caccagaatc
    50821 acgcccatcg gtttcaggaa gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc
    50881 agatagttgg cgatatagca aaacaacgac agttttgtca gctccgcagg ctggatacgc
    50941 agcaaaccga gatcgatcca acgcgatgcc cctttaacag cgctacctac taccagcacg
    51001 atcatcagca ggatgataga tccgagcagc atcgtggcac tgtagcgttg ccagaactcc
    51061 atcggcagac gcagcgtaat gatcgccaga ataaacgcca aaatcagata gacaccatca
    51121 cgcttcgcga agaagaacgg atcgttggtt aagcgttgcc ctatgggcat tgacgccgag
    51181 gtcaccatga taaagccaat cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg
    51241 tacatgatca ggctgtcggt atctttttcc cgcgagccca tcacccagcc ctttagcgcc
    51301 gtggagatcc agaccaagat actgaatcct ggcaggcgcg gcattttcag gcgagggaga
    51361 gataaacgca tcaacctaac tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa
    51421 agttcttgaa ctgatcaagg ctggcacagg ccggggagag cagaaccata tcgcccggct
    51481 gaacacgcgg agccagcaag cgcatagcct gttccatagt ttcggtttgt tctgccactt
    51541 ccgggcgtag cgccgccagc tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat
    51601 cgccattcag gtaacgcgcc agtgggctaa agtccgccga tttaccatcg ccgcccagca
    51661 acaaatgcag cgtgccgtct acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt
    51721 tggtcgcttt tgaatcgtta atccagcgca caccgttatg ctccagcacc acttcaaagc
    51781 gatgcggcag accggtgaag gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg
    51841 cggcatctgc cagcgccagc gccgccagcg cattggtgta gttatgctgc ccggaaagtt
    51901 tcatctcttt cacattcagc actttctcgc ctttaacccg cagccaggtt tcgccctgct
    51961 gatgattcag gtgatagtca cccatgttga cgccaaagct gacgcagcgt tcatccgcac
    52021 cgcgaatcgg cattgttaag gcatcatcag cattaaccac gcaaactttc gcgttttcgt
    52081 aaatgcgcag ttttgctgca cgatactgtt gtaaaccaaa cgggtagcga tccatatgat
    52141 cttcagtcac gttcagaatg gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct
    52201 ggaagctcga cagttccagc acgtacagtt cacactcatc atccagtagc atcaacgcag
    52261 gcaggccaat attgccaccc acaccaacgt taacccccgc cgctttcgcc atttcaccca
    52321 ctagcgtggt gaccgtgctt ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg
    52381 cttcgcgaca aaacagctcg atatcgccaa cgatttcgat tccggcatca gcggcagcgc
    52441 ttaaggatgg atgcgccagt gcaataccgg gactggcgac aatcagatct gccgccatca
    52501 gccattcatc attcagactg cccgtgtggc gttctacggc ttcgggtaat ttatccaggc
    52561 taggcggtgt catacgcgta tccataacgc gcggcgtcac accgcgagcg aggaaaaagt
    52621 ccacgcagga aagcccggtg aggcccaggc cgataatgac aacattttta ccttggtaat
    52681 cagccatgat taacgtacct tcagcgttgc cagaccaatc aggaccagca tcagcgaaat
    52741 aatccagaaa cgcacaatga cgcgcggttc cggccagcct ttcagttcat agtggtgatg
    52801 aatcggtgcc atgcggaaaa tacgttgtcc gcgcagttta aaggagccga cctgcaggat
    52861 gacagaaagc gtttctacca cgaacacgcc ccccataatc accagcagga attcctgacg
    52921 tagcagtacg gcgataatgc ctaacgcacc acctaacgcc agcgaaccga catcgcccat
    52981 aaagacctgc gccggatagg tgttaaacca caggaagccc agtcctgccc cgactatcgc
    53041 ggtacagaca ataaccagtt ccccggcgtg tcgcagatac ggtatgtgta aatagctggc
    53101 aaagttcatg ttgccggtcg cccacgccac cagcgcaaaa ccaccggcga caaatacggt
    53161 cggcataatt gccagaccgt caagaccatc ggtcaggttt accgcgttgc cagtacccac
    53221 aatgacgaag taagccagca gaatgtagaa cagccccagt tgcggcatca catctttaaa
    53281 gaatgggacc accagctgcg ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc
    53341 aacacccagc gcaatgaccg acatccagaa atatttccaa cgagcgatca accctttggt
    53401 atctttacgc accactttgc gataatcatc aacaaagcca ataacaccgt aacctaccag
    53461 caccaccaac acgcaccaga cgtacggatt ggacgggtaa gcccacagca gcacggagat
    53521 cacaatcgct gtcaggatca taatcccgcc catggtcggc gtaccgcgct tgctgaagtg
    53581 tgattcagga ccgtcgttac gcactacctg accaaaggaa agtttttgca aatgagcaat
    53641 catgcgcggg cccatccaca atgagatgaa cagcgcggtc agcaggctga cgatggcgcg
    53701 aaacgtcaga taggaaaaga cgttaaagcc ggaataatat ttgaccaaat gttcggccag
    53761 ccaaactaac atgtcccatt ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta
    53821 cgtgaaccct taactaaaat cgtaattacc tgttgctcag caatcagtga tttaagacgc
    53881 gtaattaacg cagttttatc agcaaaatgt tcgccaacgc cgctggcggt gctgatagca
    53941 tggctttgtt tacccatgct taacacgcgg tcaataccag cagcttttgc cgcctcgccc
    54001 acctgtacat ggcaggcttc gctttcagcg cccagttccg ccatatcgcc caccaccagc
    54061 acgcggtagc ccggcatttc agccagtacc tgaacagctg cggtcattga accgacattg
    54121 gcgttgtagg agtcgtcgag cagcaactgg ttttctgcca gttggatggg gaacagacgg
    54181 ccaggaacag ctttcagatt tgccagcccc gctttgatag catcaagcgt tgcgcccacg
    54241 gacatggaga gcgcagcggc tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc
    54301 agcagaacat cgacgctacc ggttggggtt tgtagggtaa attccatacc gtgcgaggtc
    54361 acatggatat tggtggcggt gaaatcgctg ttggcggcat tgggtgagaa acgccacact
    54421 ttgcgtgagc caattacgct ctgccagttc agccagtcgt tgttgtcggc gttcataatg
    54481 gcgataccgt tttccggcag gccgctaaag atttcacctt tcgctttcgc gacacccgca
    54541 agcgagccaa aaccttccag atgcgccgct gccaggttgt tgaccagcgc cgcttccggg
    54601 cgagtcagac tcacagtcca ggcaatctcg ccctgatggt tcgcgccaag ttcaataact
    54661 gcgtaatcgt attccggcgt taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg
    54721 agattgcctg ccgtataaag cgtgttgccg cactggctta aaatcgccgc cgtcatctct
    54781 ttaacggagg ttttgccgga ggaccccgtc agagcaacca cgcgcgccgg aacttgctgg
    54841 cgaacccatg cagccagttc accaaacgcc agacgcgtat ccttgacgat taactgcggc
    54901 aggtcgatgt ccagcggacg gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg
    54961 tcggcaaaat cgtgggcatc aaaacgttca cctttcaggg caacaaacag gcagcccggc
    55021 gtcagttttc gggtatcagt ggttacagca tcaagggtga tatctgcacc ttgcagttca
    55081 ccgttgagaa tatcggtaag ttggctaagg gttacgctaa tcatgcaatc ccccccagca
    55141 gacgcgccac cgtgacgcga tcggagtagt ccagacgctg attgccaaca atctggtaat
    55201 cctcatggcc tttgcccgcg accagtacca catcattctc tttagcctgc ataacggcgc
    55261 aagtcaccgc ttcagcacgg ccttccatca ctttggcatg tccggcatct aacattcccg
    55321 ccagaatatc gttgatgatg gcacgcggtt cttcggtacg cgggttatcg tccgtcacca
    55381 ccgccacgtc agcaaactct tcggcaattg cgcccatcag tggacgctta cctttatcgc
    55441 gatcgccacc acagccaaag acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct
    55501 gtaaggcttt ttccagtgca tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc
    55561 ctggcgcagt gaacacttcc atacgtccgc aaaccggttg cagacgcgcg gcggttttca
    55621 gcagatcagc cagtggatag cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc
    55681 tgacgttaaa agcgcccatc agatggcttt caatttcgcc atcgccccag cttgaactaa
    55741 agcgaatggt cgcgccgctg tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt
    55801 gacagttcgg attgatatga tcttccattg ataccgcaac cgcgtccggc agttttgcca
    55861 gccagcggcg gcccacttca tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag
    55921 aataaagcag ccatttcgca gcttcgtagt gttccatatc accatgataa tcaaggtgat
    55981 cgcggcttaa gttggtaaag accgacgccg gaaatttcaa tgccgccaca cggtgctgta
    56041 ccagcccgtg ggaggaaact tccattgcgc aaaaagtcgc gccctgatcc accagccccg
    56101 ccagctcatg ctgaacatca actgccgaac cggtagtatt ttctgtcggg atcactttcc
    56161 ccagcagacc attaccaacg gtgcccatta ccgcgctggt ttcgccaagc agttggctcc
    56221 actgcgccag aagctgggtc gtcgtggttt tgccgttggt gcccgttacg cctacgagac
    56281 gtaaattgtc agagggttca tggtaaaagc ggcccgccag tgcagataaa cgctcgttga
    56341 gctggctgag atagatgacc ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct
    56401 catctttcgc ctctgcaata atggcagcca caccttgcgc tatcgcctgc gggatatatc
    56461 gacgcccgtc cgcctgatga cctactacag ctacaaagag atcgcccgcc gcagccacac
    56521 ggctgtcgag tgtcatctct cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa
    56581 gaaggtcgcg caaattacga tctgccacct gtcccctcgc cttgattaat cacaaattca
    56641 tttttatcgc ccgttgtcag cgcatccggc tcgatgttca tggtacgcaa tacgccgccc
    56701 atgatggcac caaagaccgg cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga
    56761 tcgttgataa caacaaccag cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta
    56821 taagcaatat atttattgat gtagcgaccg tccggcccga cctttttcgc ggtaccggtt
    56881 ttaatggcga tacgatagcc tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc
    56941 acgctttcca tcatatgcac cacggtgcga acaatggatt ccgggaagac acgttcaccg
    57001 ggaaccgggg ggtcaacttt ggtaatcgac agtgggcgat aaatgccgta gctgccgata
    57061 gttgcgtaga ctcgcgctaa ctgtaacggt gttaccatta gcccgtagcc gaaagagaag
    57121 gtggccctct ctatgtcaga ccaccgttgt ttttgaggat ataagccact gcgttctccg
    57181 accaacccca aattggtcgc ttttcccagt ccaaaacgtg agtaagtatc tactaacgct
    57241 gaggacggca tcgctaacgc cagcttggaa acaccgacgt tactcgactt ctgtaatacc
    57301 ccggtcaggg ttaattcgct gtagcgtgcc acgtctttga tttcgtggcc gttaattcga
    57361 taaggaatgg tattgagtac cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc
    57421 accaccatcg gtttaaccgt tgagcccggt tcaaacacgt cggtgatggt acggttacgc
    57481 atcgcctctt tcggcgtgcc gctcagattg ttagggttgt atgacgggct gttagccatc
    57541 gccagcactt cacctgtgtt gacatccacc agcacggcgc taccagattc agccttgtta
    57601 aaggccaccg cgttgttcag ttcgcgataa accagcgcct gcaggcgttc atcaatactc
    57661 agcgccaggt tgtgcgctgc ctggctgtca gtagaagaaa tatcttcaat tacgcgacca
    57721 tagcggtctt tacgcacaat gcgctcaccc ggctgcccgg taagccattt atcgaaactc
    57781 ttctcaacgc cctcaatccc ttgactatcg acgttagtaa agccgatgag gtgagcagtc
    57841 acttcgccgg acggatagta acggcgagac tcttcacgca gatgaatccc cggcagtttc
    57901 agttttttga tgtagtccgc catgtcagag tttacctgac gcgccagata aataaagcgc
    57961 cctttcgggt tggcgttaat gcgggcagaa agctgatcca gcggaatatt gagcgcgtta
    58021 gccagcgcct tccagcggtc accgacgctg ataccgccag cgtcatgcac ttctttcggg
    58081 tcagcccaaa tcgcttttac cggcacgctc accgctaacg ggcgaccaga acggtcagta
    58141 atcatgccgc gggaggtgga aacttgctga acgcgaagag aacgcatgtc gccctctttc
    58201 accagcatat ccggggagat aacttgtaac cacgctacgc gtccgagcag aaaagccagc
    58261 gccaggagaa tacagccgca taacaacgca aaacgccaac tgataaagtt ggcatgttct
    58321 tcctgacgtt ttggtttctg cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat
    58381 ttttgcacta cgatattttc ttgtgacgga tcaacatgct gcatttgcag cttttccgtg
    58441 gcgatccttt ccacccggct atggtcgccg agcgcattct cttcaaggat caggttgcgc
    58501 cattcaatgt ctaaagcatc tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa
    58561 cgggtatggt gcgccgtggt taccacggtc accgccgtca aaataatgca aatgaacagg
    58621 cagagtggca gcttcccaaa tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg
    58681 cgctcgtggc ttcccatcga tcctttaact ttgcttagag cttctgtcac tctgctgatc
    58741 atgcgttcgt cctctctgca atacgcagaa ctgaactacg ggcacgaggg ttctcagcca
    58801 cctcttcttc gcccggcatt aacttgccta gtgctcgcag ctgacggcca cccagttttt
    58861 tgagctgctc ttcagtcatc ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac
    58921 gcataaaacg tttcacaata cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc
    58981 cacccggggc cagcacgttg agcgagcttt ttagcgcctg ctctatctcc tccagttcac
    59041 tgtttaccca aatgcgcacc gcctggaagg tacgggtcgc gggatgttta aacttatctt
    59101 tcaccggcgt tgcagcagcc acgacttccg ccagttcttt ggtgcgggtc atcggctgtt
    59161 cgcggttacg ctcgacaatg gcgcgggcaa tgcgtttggc aaaacgctct tcaccatagg
    59221 ttttcaatac ccaggcgata tcggcttctt ctgcggtttg tagccattca gcggctgact
    59281 gcccacgggt tgggtccata cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc
    59341 gttcagcatc atcaagttgc ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct
    59401 tgccaataag atcgcgctcg gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga
    59461 tggagaagcg cggatcatca atagtcttcg caacggcgat agcctgcggg tcgcgatcga
    59521 tcgccagcaa acgcccctct tcgccaagct gcgagaggat cagacgtgag tgaccaccgc
    59581 gaccaaaagt cccatcaatg tagatgccat caggacggat attgaggcca ttaacggctt
    59641 catccagcag caccgtagta tgtttatagt tttccatcat tttatagaga caagtcctgc
    59701 agtcgctccg ataagtctcc ggtagccaac tgctctgcgt cgatatcttc cttgacctgt
    59761 tgatgccagg ttgtttcatc ccacagctca aacttgttga actgtccaac cagcatcact
    59821 tcttttgtca gcccggcatg ttgccgcagt actggcgcga ttaacaatcg acctgcgcca
    59881 tccatctgac attcgctggc atgacctaac agtaggcgct gcacacggcg ctcaaccggg
    59941 ttcatgctcg acagacgcga taatttttgc tcgataattt cccattcagg cagggggtaa
    60001 agcagcaggc acgggtgatg aatgtcaatg gcgcaaacca tttgaccggc agcgttctca
    60061 agcagctgtt cccgataacg ggtaggcact gataagcgcc ctttgctgtc gagattgact
    60121 aacgttgctc cccggaacat gccagcctca ccccttattc ccactttgcc ccacaatttc
    60181 ccactgaaag gagtttacgg agcggaggaa aagcttgtca agccagtcac aaggcatacc
    60241 gaggtaaaaa acccgatgtt tacggctaat atcagcgttg tttattttac gtccaacgtg
    60301 cgaagcaaat taacgttatg aatagctgta agaaaaaatc cctcatcgtc atcactgttt
    60361 taaaatgact cactattttc cacagcaaat ataaagtgtc agtttgcgac gcgagcggca
    60421 ttttaggaca tatcttcccc ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc
    60481 agcgggtttg cgcaaaagca aagaaaagtg tttgttaaat cggcaattca tcgccgcatg
    60541 taacaaaata atgcaaaaac gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg
    60601 tgagttaatt aacgggcggg tttaaaacca tttatcaata atagtcagga atagtcttat
    60661 ttactttaa
//
LOCUS       DAJTYG010000012        59523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTYG010000012 DAJTYG010000000
VERSION     DAJTYG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.012"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO568_000814"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO568_000814"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6640281.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO568_000815"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO568_000815"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6640282.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO568_000816"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO568_000816"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6640283.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO568_000817"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO568_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6640284.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO568_000818"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO568_000818"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6640285.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO568_000819"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO568_000819"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6640286.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO568_000820"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO568_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6640287.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO568_000821"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO568_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6640288.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO568_000822"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO568_000822"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6640289.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO568_000823"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO568_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6640290.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO568_000824"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO568_000824"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6640291.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO568_000825"
     CDS             complement(15665..16702)
                     /locus_tag="OO568_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6640292.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17034..17522
                     /gene="yhhY"
                     /locus_tag="OO568_000826"
     CDS             17034..17522
                     /gene="yhhY"
                     /locus_tag="OO568_000826"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6640293.1"
                     /translation="MSEIVIRHAETRDYETIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17581..19303)
                     /gene="ggt"
                     /locus_tag="OO568_000827"
                     /pseudo
     CDS             complement(17581..19303)
                     /gene="ggt"
                     /locus_tag="OO568_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            19423..19863
                     /locus_tag="OO568_000828"
     CDS             19423..19863
                     /locus_tag="OO568_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6640294.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19850..20593)
                     /gene="ugpQ"
                     /locus_tag="OO568_000829"
     CDS             complement(19850..20593)
                     /gene="ugpQ"
                     /locus_tag="OO568_000829"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6640295.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20590..21580)
                     /locus_tag="OO568_000830"
                     /pseudo
     CDS             complement(20590..21580)
                     /locus_tag="OO568_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21582..22427)
                     /gene="ugpE"
                     /locus_tag="OO568_000831"
     CDS             complement(21582..22427)
                     /gene="ugpE"
                     /locus_tag="OO568_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6640296.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22424..23311)
                     /gene="ugpA"
                     /locus_tag="OO568_000832"
     CDS             complement(22424..23311)
                     /gene="ugpA"
                     /locus_tag="OO568_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6640297.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23409..24725)
                     /gene="ugpB"
                     /locus_tag="OO568_000833"
     CDS             complement(23409..24725)
                     /gene="ugpB"
                     /locus_tag="OO568_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR6640298.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25122..25835)
                     /gene="livF"
                     /locus_tag="OO568_000834"
     CDS             complement(25122..25835)
                     /gene="livF"
                     /locus_tag="OO568_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6640299.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25837..26604)
                     /gene="livG"
                     /locus_tag="OO568_000835"
     CDS             complement(25837..26604)
                     /gene="livG"
                     /locus_tag="OO568_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6640300.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26601..27878)
                     /gene="livM"
                     /locus_tag="OO568_000836"
     CDS             complement(26601..27878)
                     /gene="livM"
                     /locus_tag="OO568_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6640301.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27875..28801)
                     /gene="livH"
                     /locus_tag="OO568_000837"
     CDS             complement(27875..28801)
                     /gene="livH"
                     /locus_tag="OO568_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6640302.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28849..29958)
                     /gene="livK"
                     /locus_tag="OO568_000838"
     CDS             complement(28849..29958)
                     /gene="livK"
                     /locus_tag="OO568_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6640303.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLACSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30382..30765
                     /gene="panM"
                     /locus_tag="OO568_000839"
     CDS             30382..30765
                     /gene="panM"
                     /locus_tag="OO568_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6640304.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30964..32067)
                     /gene="livJ"
                     /locus_tag="OO568_000840"
     CDS             complement(30964..32067)
                     /gene="livJ"
                     /locus_tag="OO568_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6640305.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32338..33192)
                     /gene="rpoH"
                     /locus_tag="OO568_000841"
     CDS             complement(32338..33192)
                     /gene="rpoH"
                     /locus_tag="OO568_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6640306.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33437..34495)
                     /gene="ftsX"
                     /locus_tag="OO568_000842"
     CDS             complement(33437..34495)
                     /gene="ftsX"
                     /locus_tag="OO568_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6640307.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIGEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34488..35156)
                     /gene="ftsE"
                     /locus_tag="OO568_000843"
     CDS             complement(34488..35156)
                     /gene="ftsE"
                     /locus_tag="OO568_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6640308.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35159..36655)
                     /gene="ftsY"
                     /locus_tag="OO568_000844"
     CDS             complement(35159..36655)
                     /gene="ftsY"
                     /locus_tag="OO568_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6640309.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36805..37401
                     /gene="rsmD"
                     /locus_tag="OO568_000845"
     CDS             36805..37401
                     /gene="rsmD"
                     /locus_tag="OO568_000845"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6640310.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37391..37660
                     /locus_tag="OO568_000846"
     CDS             37391..37660
                     /locus_tag="OO568_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6640311.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37663..38022)
                     /locus_tag="OO568_000847"
     CDS             complement(37663..38022)
                     /locus_tag="OO568_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6640312.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38163..38789
                     /locus_tag="OO568_000848"
     CDS             38163..38789
                     /locus_tag="OO568_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6640313.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38863..41061
                     /gene="zntA"
                     /locus_tag="OO568_000849"
     CDS             38863..41061
                     /gene="zntA"
                     /locus_tag="OO568_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6640314.1"
                     /translation="MSTPDNHGKKAPQFAAFKPITTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41163..41408)
                     /gene="tusA"
                     /locus_tag="OO568_000850"
     CDS             complement(41163..41408)
                     /gene="tusA"
                     /locus_tag="OO568_000850"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6640315.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41629..42294
                     /locus_tag="OO568_000851"
     CDS             41629..42294
                     /locus_tag="OO568_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6640316.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42367..42924
                     /gene="dcrB"
                     /locus_tag="OO568_000852"
     CDS             42367..42924
                     /gene="dcrB"
                     /locus_tag="OO568_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6640317.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42928..44145)
                     /locus_tag="OO568_000853"
                     /pseudo
     CDS             complement(42928..44145)
                     /locus_tag="OO568_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            44277..45326
                     /locus_tag="OO568_000854"
     CDS             44277..45326
                     /locus_tag="OO568_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6640318.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTRVCKIWMETTKGGSKL
                     AILLGPDRPKSRLPG"
     gene            45381..45968
                     /gene="acpT"
                     /locus_tag="OO568_000855"
     CDS             45381..45968
                     /gene="acpT"
                     /locus_tag="OO568_000855"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6640319.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46079..47653
                     /gene="nikA"
                     /locus_tag="OO568_000856"
     CDS             46079..47653
                     /gene="nikA"
                     /locus_tag="OO568_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6640320.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPLETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47653..48597
                     /gene="nikB"
                     /locus_tag="OO568_000857"
     CDS             47653..48597
                     /gene="nikB"
                     /locus_tag="OO568_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6640321.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPTTL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48594..49427
                     /gene="nikC"
                     /locus_tag="OO568_000858"
     CDS             48594..49427
                     /gene="nikC"
                     /locus_tag="OO568_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6640322.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49427..50191
                     /gene="nikD"
                     /locus_tag="OO568_000859"
     CDS             49427..50191
                     /gene="nikD"
                     /locus_tag="OO568_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6640323.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50188..50994
                     /gene="nikE"
                     /locus_tag="OO568_000860"
     CDS             50188..50994
                     /gene="nikE"
                     /locus_tag="OO568_000860"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6640324.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKNLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51000..51401
                     /gene="nikR"
                     /locus_tag="OO568_000861"
     CDS             51000..51401
                     /gene="nikR"
                     /locus_tag="OO568_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6640325.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51521..51880)
                     /locus_tag="OO568_000862"
     CDS             complement(51521..51880)
                     /locus_tag="OO568_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6640326.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51877..52152)
                     /locus_tag="OO568_000863"
     CDS             complement(51877..52152)
                     /locus_tag="OO568_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR6640327.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(52211..53335)
                     /locus_tag="OO568_000864"
     CDS             complement(52211..53335)
                     /locus_tag="OO568_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6640328.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53335..56070)
                     /gene="rbbA"
                     /locus_tag="OO568_000865"
     CDS             complement(53335..56070)
                     /gene="rbbA"
                     /locus_tag="OO568_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6640329.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQSFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQLTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56067..57134)
                     /locus_tag="OO568_000866"
     CDS             complement(56067..57134)
                     /locus_tag="OO568_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6640330.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKTAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57500..59122)
                     /locus_tag="OO568_000867"
     CDS             complement(57500..59122)
                     /locus_tag="OO568_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6640331.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNHGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDSSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDANVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGVQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINLGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
BASE COUNT        14066 a        16427 c        15077 g        13953 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggaatgg tttttatctt ccactgcagc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cgaatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccacacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgtttgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tgcctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcaga cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tattacgggt aactgttatc ggtaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctggat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tagcaaaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc
    16861 aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat
    16921 aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc
    16981 atttgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg
    17041 agatagtaat acgccacgca gaaacacggg attacgagac catcaggcag attcacgccc
    17101 agccggaggt gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg
    17161 agcgactcgc cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg
    17221 tgggccatct caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg
    17281 gtatctgtgt cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga
    17341 tgattgaaat gtgcgacaac tggttacggg tagatcgcat tgaactaacc gtgtttgtcg
    17401 ataacgcgcc agcaattaag gtctataaaa aattcggctt tgagattgaa gggaccggta
    17461 agaagtacgc attgcgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaaat
    17521 agagaatttg caatctaagt ttgtcattta agccctcacc taccgggagg gcttaagata
    17581 ttaatacccc gccgttaaat catccaccga gcgcgggtcg gatacgccgt acaactcacc
    17641 gtccggccca accataatgc tttgtgtgct gcccatcgcc tctttcagcg ccactttctg
    17701 accttttgct tccagcagct tgagcgtatc cgggctaaac cctttttcga cacgcagctc
    17761 gtccggcaac cactgatggt ggaaacgcgg cgcattggtc gcttcggcga cgttcatgcc
    17821 ataatcgatg ctattcacca ccatttgcag caccgtggtg ataatccggc taccgcctgg
    17881 gctaccggta accagccagg ttttaccgtc tttcaccaca atggtcggcg acatcgacga
    17941 cagcgggcgt ttgttcggcc cgacggcgtt ggcatcaccg cccaccagcc cgtaaacgtt
    18001 cggtacgccc ggtttggcgg agaaatcatc catctggtta ttaagcagaa taccgctctc
    18061 gcccgcgaca atacccgtac cgaaggtggt gttcagcgta taggtcaccg ccaccgcgtt
    18121 accgtcttta tccactacag agtaatgggt agtttgatta ctctcataag gcgcaagctt
    18181 gccagggcga atctcactgg acggcttcgc ttttttgata tcaatttgat cggcaatgga
    18241 tttggcatag gctttattgg tcagtgcctg ccacgggact ttgacaaaat ccgggtcgcc
    18301 aagatattcc gagcggtcgg cgtaggcgta tttctccgct tctgccatga tttgcatcgc
    18361 atcggcgctg ccaaagccgt atttcttcat atcgaagttt tccagaatat tgaggatttg
    18421 tacgatatgg atcccgccgg aggatggcgg tggcatggag taaacctgat accagcgata
    18481 atcgccgctt atcggagtgc gttcgaccgc tttataggcc gctaaatctt ctttagtgat
    18541 caagccaccg tttttctgca tctcctgggc gatctgttcc gcaatcgtgc ctttatagaa
    18601 ttcgtccggg ccgttttcag caatcatctc caggctcttt gccaggttcg cctgcaccag
    18661 cgtgccgctc tttttcagcg gctcgccctc tttccagaag atagctttac tgttttcgtg
    18721 attcggcaac acttcgctac cgtaggtttt gagatcgtca gccagcgcgt cgttaacgat
    18781 aaaaccatcg cgtgccagtt taaacgcggg ctgcacgact ttgttcagcg gcatggtgcc
    18841 gtatttatcc agcgccagcg agaaacctgc taccgtaccc ggtgtgccgg aagccagatg
    18901 cgaagtgagt gattttttgc tgtccgggtt gccctgatca tcgaggaaca tatcgcgggt
    18961 cgctttggcg ggtgccattt cgcggaaatc gatagccgtg gtattgccat ttttcgagcg
    19021 gattaacata aaaccaccac cgcccagatt ccctgcctgc ggatgcgtta ccgccagcgc
    19081 gtagcccacc gccacggcgg catcaacggc attcccgccc tccttgagga tatccacccc
    19141 cacctgagtg gcagtggcgt ccacagacgc taccattccc tgtttcgcgc gtaccggatg
    19201 gaagacatct tcctccacac catacgagac gggcggcgct aaaacaactt cctgagagca
    19261 gagcagcaat ggccacccgg cgtaaaaacg tcggttttat catcgttatt ctccagagat
    19321 taaggggcaa ccccagctaa gcctggtata taactctgaa ttaatcatcg ttttgtcggg
    19381 aagcgagtaa acttaaagga tatcttcaga ggaggatacg cgatgaaacg acttctgatt
    19441 cttacggcac tcctgccgtt tgtcggcttt gcacagccca ttaatactct gaacaaccct
    19501 aaccagccgg ggtatcagat acccagccag cagcggatgc aaacccagat gcagactcag
    19561 caaatccagc aaaaagggat gctgaatcag caactgaaaa cgcaaactca gttgcaacag
    19621 cagcatttag aaaaccagat aaacaataat tctcagcggg tgttgcagtc acagccgggg
    19681 gagcgaaatc ccgcccggca gcaaatgctg cccaacacca acggcgggat gttaaacagc
    19741 aaccgtaatc cggatagttc gttgaatcag cagcatatgc tgccggagag gagaaacggc
    19801 gacatgctga atcagcccag cacgccgcag cctgacattc cgttgaaaac tattgggccg
    19861 taaagttcgg accaatcacg tcaatcgcat cggtacagat gcaatccacg ccccagcgca
    19921 gcaactctgc cgcgcgctgg ggtttattga cggtataaac cagaatccgt aatccggcgt
    19981 ctttcaactg catcactcgc gctttatcga gtaacttatg attgagatga atagagacgc
    20041 agcccagccg cgcggtcagt tcgcgccagt cgtcgcgcca ctcatcaagc aataaaccac
    20101 gcggcagttc cggtgccgcc tgttgtgcag cttctaaagc atcaatctca aacgatgaca
    20161 gcagcggcgg cgtcataccg gcccacagtt ggcgtgccgc cagcgccacc attttgcccg
    20221 ttaatggtcc agtgccagtg gtgggtttga tttcgatatt cgccatcatc ccgtgttcgc
    20281 gacagcgttc cgccacctgc gaaagcaacg gcagcggctc acctttaaac gctttgctgt
    20341 accaactgcc cgcatccacg cgcagtaaat cctgccagtt cagttcaccc gcgacgcccc
    20401 agccgttgct ggtgcgttcg agattgtcgt catggagcag gaagatctcg ccatctttcg
    20461 ataacttcgc gtcaaattcg atcatcttat gaccgtattt tgccccgacg tcgattgccg
    20521 ccagggtgtt ttccggggcc agcttaccgc cgccacgatg agcgacgatg cggggataag
    20581 gccagttact catactcgtt gtcctgtttc accatcaaaa agatgcagct gattttccgc
    20641 cagatgcagc cacagcgtgc tgcctgccgt cgggcgctcc tgatgcgcca gtcgcaccac
    20701 cagcttctgc ttgcgagctt agcgcaatat gttccgggcg aatgccgagg gtcattttac
    20761 gcccggcata ctgacggtag ccaccgttta gcggtagcac gataccgccg tccagttcga
    20821 aatgcgtgcc ttcattattc atgcggcctg tcagcaggtt catcgccggg ctgccgataa
    20881 aactcgccac aaacaggctg gcgggctttt cgtagacttc caccggcgtg ccaatctgtt
    20941 cggcaacgcc gccgttcatc accattactc gctgggcgag cgtcatcgcc tcaacctgat
    21001 cgtgagtaac gtagagtgaa gtcgttttca ggcgacggtg cagctgttgc agttcaagac
    21061 gcatctgtac gcgcagcttg gcatcgaggt tagagagtgg ctcatcaaac aagaacaccg
    21121 ccggatcacg cacaatcgcg cgtcccatcg ccacacgctg gcgctgaccg ccggaaagct
    21181 cgcgcgggcg acgtttgagc agaccgtcca gctccagaat gcgcgccgct tctttaacgc
    21241 gctcggctat ttgctgcttg cccatgccgc gaattttcag cccccacgcc atgttttctt
    21301 cgacgctcat atgcggataa agcgcgtaat tctggaacac catcgcaatc ccgcgatctt
    21361 tcggctccat ttcggtcacg cgctggtcgt tgatccagat atcgccttcg gtcaccctct
    21421 ccagcccggc aaccatacgc agcagcgtcg atttcccgca gccagacggc ccgaccatca
    21481 cgataaattc gccatccgcc acatcaaggg tcagcggttt aatgacctag gttttgccat
    21541 cccagctttt ggttactgcc tgtaatttca gtcctgccat cttatttctc actatcgacc
    21601 aggccgcgca cgaaggcacg ctgcatcact aaaacaatca ctactggtgg gatcagggtt
    21661 aacaacatcg ccgccatcac tgagttccat tccgtggtgc cttcgccagt ggcgatcatc
    21721 cctttgatcc ctgccacggt ggtgccgaga tccacgtcgg taataatcaa caacggccac
    21781 aaatactggt tccagccgta aataaaggtg atcacaaaca gcgccgccag attagttttg
    21841 gagagcggaa aaacgatgtc gcaaaagaag cgcattggtg acgcaccgtc gatccgcgcc
    21901 gcttccacca gttcatccgg cagcgtcata aagaactggc gaaacagaaa cgtggcggtc
    21961 gccgaggcca tcagcggcag cgttaaaccg gcgtagctgt cgagcatctt caggttggcg
    22021 atgacttcca ccgtcgggaa gatacgtact tcaaccggca gcatcagggt gataaaaatc
    22081 atccagaaga agaggttacg tagcggaaaa cgaaaccaga caatggcaaa tgccgagagc
    22141 atcgagacag taattttgcc gagcgtaatg ctgaacgcca tcacaaagct gttaagcaac
    22201 atccgccaga acggcgcgct atttgtgcct accccgttca cccagatgtt gtggatgttt
    22261 tccagcagat gcgtgccggg gatgagcgtc atcggcgcgg cgtagacggc ctgtttatcc
    22321 agcgtcgccg cgacaaacgc cacgtacagc gggaagagga tcaccgcgat cccgaggatc
    22381 agcatggtat ggctgaatat cgtcagccac ggacggttct caatcattgg taacgcacct
    22441 tgctttcaac atagcggaac tgcaccaccg tcagcacgat gacgaggaac atcaacacca
    22501 ccgactgtgc ggcagacgaa gccagatcca gtccggtaaa accttcgcgg tagatcttat
    22561 aaatcagcgt cgtggtggcc tgaaccggcc cgccggacgt ggcggcgtcg atcaccggga
    22621 aggtgtcgaa gaaggcatac accaggttca ctaccagcag gaagaaactc accggggcga
    22681 taagcggcag cgcaatctta aagaagcggc gaatcggccc tgcaccgtcg atggctgcgg
    22741 cttcgatcaa cgaacgggga atggattgca gcgcggcata gaagaacaga aagttgtagc
    22801 tgatttgctt ccacaccgag gcaaacacca ccagaaacat cgcctgaccg ctattttgcg
    22861 cgtggttcca gtcgtagccg aactcagcga gaaaatgggt gatcagcccg cgaccggggt
    22921 taaacaggaa gatccacaat acggcggcaa cggcgggagc cacggcatac ggcagcaaca
    22981 ttaaggtttg gtagaaacga ctgccgcgca cgatgtactc caccagcgcc gcaaagaaca
    23041 gcgataccag caaaccgctg acggtgacaa aggtgctgaa ttttatcgtc gtccagaagg
    23101 agtcgagata atagctgtca tgaaacagcg tgacgaagtt atccagaccg acaaactggc
    23161 tggagaaacc aaacggatcg acgctttgta gcgagtacca caacgcttcg cccgcaggcc
    23221 agataaaaaa gataacggtg atgatgagct gcggcgcgac cagcagataa ggcagccagc
    23281 gcgagcggaa caccggacgg gatgatgaca ttacggttaa ttcctgaact gtgccggatg
    23341 cgcttcgctt atccggccta cacgcgatgc caggtcagat aagcgaaacg catccggcat
    23401 tacactgatt aagacttcgt cgatttctca aagcggcgca gtaactggtt cccacgctca
    23461 actgcggtat ccagcgcctg ctgtggcgtc ttcttaccgg tccacacgct ctcaagctct
    23521 tcatccacaa tcacgcggat ctgcggcatg ttgcccagac gaagcccttt ggtgaacggc
    23581 aacggcggct tgttcagcat ctgacgcgtc gcggtatccg cacctgggtt tttctcgtaa
    23641 aagccctgct cacgggtcag gtcatacgcc gctttggtaa ttggcagata accggttttc
    23701 tgatgccact cagcagcgtt ttctggcttc gcgaggaagt cgagaaactt cgccacgccg
    23761 gtatacgttt ctttatcttt gccctgcatc acccacaggc tggctccgcc gataatggcg
    23821 ttttgtggcg catctttcgc atcggcgtcg taaggcatca tacctacgcc gtagttaaat
    23881 ttggcgtact cgcgaatgtt ggcaagagaa ccggaagagg cggtggtcat cgcgcaatca
    23941 ccgttataga acttctcggt ggattcatcc ttacgaccga cgtagctgaa gtcgcccttc
    24001 ttgttcatct cctcgagcat ggcgatgtgt ttcacctgct ccggcttgtt gaactccagc
    24061 accgcgtccg tgccgtcaaa gccgttgttt ttgctggcaa acggcagacc gttccaggcg
    24121 ctaaagtttt ccagttggat ccagccctgc cagccgctgg cgtagccgca cttaataccg
    24181 gaggctttca gttttgcgga atagtccgcc agatcctgcc aggttttcgg cggctgttcc
    24241 gggtctaacc ccgctttctt gaaggcgtct ttgttgtaat agagaacggg agtcgagctg
    24301 ttgaacggct gggagagtaa gtggccggtt ttgctgtcgg agtagtaacc ggaaaccgtc
    24361 ggcacaaact gcgactcatc gaactgaatc cctgcctctt taaacacgtc atacaccggt
    24421 ttaatggctt tcgacgccat catggtggcg gtgccaactt cataaacctg caaaatagcc
    24481 ggagcgttgc cggtacgaaa tgcggcaatc cccgcgctta aattctgttc gtagttgcct
    24541 ttataggtcg gtacaatttt gtaatccgga ttttcggcgt taaaacgttg ggccagagaa
    24601 tccacctctt tacccagttc cccttccata gaatgccaga acggaatggt cgtcactgcc
    24661 tgtgcattcc ccattaacgc cagtccgagc gccagtgctg aagctgtata atgtaacggt
    24721 ttcatcgttt atctctcttg ttgtaccgaa tgcgcgaatt cacgcgtttt atgctcgcgg
    24781 ggtaacatga catgctcgaa ttacagaaaa ataacttttt tgttacattt gtaagatagt
    24841 aaggtgtcag aaagatgaca aggcggtgac ggcgtgggtg agggaaaatg ggagatgggg
    24901 cacggataag cggaaaaata gaaggtctga atcaaactct acagattact catcgtttca
    24961 tgccggatgc ggcgtaaacg ccttatccgg cctacaagat cgtgcaaatt caatatattg
    25021 caactcaccc agtaggcctg ataagcgcag cgcatcaggc aattttgcat ttgtcacctg
    25081 tctcaaagga gtcttttgac tccttatcaa tcaacgtgtt attacccgcc taaatacgca
    25141 cttctcactg cttcgttcgc cagcagcgcg tcgccggtat cggagagcac cacatggccg
    25201 ttttccagca cgtagccgcg atccgccagc tttagcgcct ggttggcgtt ctgctcgacg
    25261 aggaagatag tcatcccctg ctcgcgcagc tgctcgatgg tgtcgaaaat ttgctggatg
    25321 ataatcggcg caagaccgag cgatggctca tcaagtaaca acaaacgcgg attgctcatc
    25381 agcgcgcgac caatcgccag catctgctgt tcaccgccgg acatggtgcc cgcccgctga
    25441 acacggcgct catgcagacg tggaaacagt tcatacaccc actttatgcg ctcctgaaac
    25501 tggtcacgtt cggcaaagaa gccgcccatc gccaggttct cttccaccgt catccgcgag
    25561 aagacgcgac gcccttccgg gacaatcgct accgcttcgc gcatgatttt cgccgtctgc
    25621 cagtcggtaa tgtctttatc atcaaacaca attcgcccgc tggtggcgcg cggatcgccg
    25681 cataacgtgc cgagcaaggt ggttttcccc gccccgttcg cgccaatgag agtgacaatc
    25741 tcgccctgat tgatatgcag gctcacctca tgcagcgcct ggattttgcc gtagtgggcg
    25801 ctgactttgt caaaggacaa catgactttt tccatcttat gcctcaccta aataggcacg
    25861 gatcacgtcc gggttattac ggatttgctc cggcgtaccg tttgccagtg gcgtcccctg
    25921 attgaccaca taaattcggt ctgaaatccc catcaccagc ttcatatcgt gttcaatcaa
    25981 caagatagtg gtgttgtgat gattgcgcag ttcggcaatc agctcatcca gctctttggt
    26041 ttcttttggg ttaagacctg ccgccggttc gtcgagcatt aaaatctccg gctgcgtcac
    26101 catgcagcgg gcaatctcca gacggcgttg gtcaccatag gccaggttac tcgcctgacg
    26161 gttggcgtgt tccagcaaac caatgcgctc aagccaggtg gcggcgcggt cgagcgcttc
    26221 gctctgggcg cgacggaaag aaggcgtttt caacaggcca gagaacagcc cggttttcag
    26281 ttgctgatgc tgcgccacca gcaggttttc aattaccgtc atttcacgga acagacgcac
    26341 atgctggaag gtgcgcacca cgcccatgcg agcaatttgc tgccccggta aaccttcaag
    26401 atgctgatcg cgcagtaaaa tggtgccgcc ggtgggtttg tagaaaccgg tcaggcagtt
    26461 aaaaaccgtg gtttttccgg caccgttagg gccgattaac gagacgattt cctgcgggta
    26521 cagttcaaga ttgacgttgt tcaccgccag caggccgccg aagcgcatca tcagaccgtt
    26581 aacagataat aatggctgac tcatgcctgc tctcctttcg ctgcgccgtt tttcagcttc
    26641 agttgcgggc gcgtcatggg cagcaaaccc tgcggacgcc agatcatcat cagcaccatc
    26701 aaaccaccga gcattaacat gctgtattcg ttgaaatcac gcatcaattc gcgcgacacc
    26761 accagcagaa cagccgccag aatcactgca aattgcgagc ccataccgcc gagcaccact
    26821 atcgccagca caaaggccga ttcagcaaag gtgaaggatt ccgggctgac aaagccctga
    26881 cgcgccgcaa acagcgttcc ggcaaaaccg gcaaatgcgg cgctgatggt aaaagcggtc
    26941 agcttgatac ggcgcgggct taagcccagc gaacgacagg cgatctcgtc ttcacgcaac
    27001 gcttcccacg cgcggcccag cggcatccgc agcaggcggt taatgacaaa caggcttaac
    27061 accaccagca gcaacgccac caggtagagg aagataacgc gatcggacgg atcgtatttc
    27121 aggccaaaga aattactgaa cgtgtcccag ccgccttcac gagcggtacg gctgaactcg
    27181 agtccgaaga gtgtcggttt cgggatctga ctgattccgt tcgggccgcc ggtgatttca
    27241 gtgttattga gcagcaatat gcgcacaatt tcgccgaaac caagggtaac gatcgccaga
    27301 tagtcaccgc gcagacgcag caccggaaaa ccgagcagga aacccgccgc cgctgccatc
    27361 agtccggcaa tcggcaaaca ggtccagaac cccagaccgt aatagtgatt gagcagcgca
    27421 aaagtgtaag cgccgatggc gtaaaaaccg ccgtacccca gcaccagcag accagaaaga
    27481 ccaacaacca cattcagccc gagaccgagg ataatgtaga tcatggtcag agtggcgata
    27541 tccaccgtcc cacgtgacac cataaacggc cacgccacca caagcaccag cagcgccacg
    27601 aggaacagct tttgcttcac cgtggaccca tcaatcgcgg gcagaataaa cttcggcccg
    27661 gaaacgcttt tcaacccttt ctggaaagcc ggtcgcaaaa gctggaagaa aaagaccacc
    27721 gccgtgccga taaacatcca ctgccaacgg atgtctgaag ccgtgtcgac caccagtttg
    27781 gtgccatcca gctccagttg tacgcccata aagacgcccg ccaggacaaa gaacatcgcg
    27841 gcagagagca gtgccattgc aatatgcatc ggtttcatac tttctctacc tccgggcgac
    27901 ccagaatacc ggtcggcatc accagcagca ccagaatcag cagggcgaat gagaccacat
    27961 ctttatattc cgtactcaga taggcagaag agagcgcctc cgcaatcccc aggatcagcc
    28021 cgccaatcat cgcccctgga atgctgccaa tcccaccgag caccgcagcg gtaaaggctt
    28081 tcatcccggc cataaagccg atataggggt taatgacgca gtagaactga ccgagcagta
    28141 cgccagccac tgccgccatc gccgcgccaa taacaaaggt caaagcaatc acccggtcgg
    28201 tgttgatgcc aagcaggctc gccattttca gatcttccgc gcaggcacga caggcgcgcc
    28261 ccatacggga atagcgaatg aaaatcgtca gcgccagcat ggcgaggaag gtgacaatcc
    28321 agatcaccgc ctgcatggtg gtaatagagg cagagaagtt ttcgctatgg cccaccaccc
    28381 actgaccgtt aaacaggctc ggcagcgcca cgtcgcgcga accttcggtc aggctgacgt
    28441 agttttgcag gaagatggac ataccgattg cggaaataag cgcaatcagg cgcttagagt
    28501 tacgcaccgg gcggtaagct acccgttcga tactccagcc gtaggcgctg gcgatgacga
    28561 ttgcgccgac gaatcccgcc gccaccagca gccagccggt atcaatgccc atcatcatca
    28621 gcgcggcgat gatcataaat gagacgtagc tgccaatcat ataaacctcg ccgtgggcga
    28681 agttgatcat gccgataatg ccgtaaacca tggtgtagcc gatggctatc agcgcgtagg
    28741 tactgcccag cgtgacgccg ttaaacatct gctgcaagaa atacaaaaac tgctcagaca
    28801 taaggtaacc tttctaaacc cgcccgcatt ttacgggcgg tgggatgatc acttggctgc
    28861 cgtggatgaa ccgtcggcgt gccactggaa gacaccaaaa tcaaatccct taagatcgcc
    28921 tttttcatcc cagttcagcg gcccaatcac ggtgtttgca ccgttagctt ttaaatcttt
    28981 caccagcgcc agcggctcat cgctgccggt acgctcaagg gcagttgcca gagattgcac
    29041 tgccgcgtaa gtgatccaga cataaggccc ggacggatct ttcttgtctg ctttcagcgc
    29101 atcaacgatg ccctggtttg ccggatcctg gtcatagcgt tttggcatag tgaccaacat
    29161 gccttcggcg gcatcaccgg caatgttcga cagcgatgcg ttaccgacac cttccggccc
    29221 cataaactgg gttttcaggc caacggaacg ggcttggcgc agcatctgcc ccatttccgg
    29281 gtagtaaccg ccgtagtaaa cgaagtcgat attttctttt ttcaggcggg cgatcagcgc
    29341 ggagaaatct ttctccccgg cggtaatacc gtcgaagaag acgacgttgg cgttagccgc
    29401 tttcagcccg tcctgcaccg aacacgccag cccttcgcca tactgttgtt tgtcgtgaat
    29461 gatggcgatg cgctggggct tcaccgtctc aagaatgtat tttgccgccg taggcccctg
    29521 ggaagagtcc agcccggcag tacgcataat gtgttgataa ccgcgttggg tcagctccgg
    29581 gttggtcgct cccggcgaga tcatcagaat accttcgtct tcgtagatat ccgacgctgg
    29641 ctgggtagag gaagaacaca gatgaccaat aacgtattta atgccgtcgt taacgatttt
    29701 gttggcgacc gcaacggctt gtttcgggtc gcaggcgtcg tcatattcca cgccaaccag
    29761 cttgtcgccc ttaattcccc ctttgacatt aatgtcttta atcgcctgac gcgcgccgtt
    29821 aaattccata tcgccccact gggcaatcgg gccggacatc gcgccgacaa cggcgacttt
    29881 aatatcgtca gccatagcgg tgtgtgaaat tgccagtgca atcatccctg cgatgatagt
    29941 tttcgcattc cgtttcatag tcaaaaatcc ccattcgtga tgttgtgttg ctttgttttt
    30001 atgtgttaac aaatcagact gttctttttt tatactgcac tgtttttgcc tgtctgatta
    30061 aggggttagc gcagtatttt gtgatgatag cgattaaaat ccctattttt cagtcgatta
    30121 agaacagata atattctgaa tttattgata gataaacaga aaaaagtgcc tttgtcagca
    30181 taaaataacg gcacaaaggg cggaataatt cactatcatt caggggatta tgctggacat
    30241 ttttcattct ctaatgtttt aactttgtaa ttattgctgt taaaaaatta atcacctgcc
    30301 aaaagaaata aaaaagagaa agcctccgat taaattattt cgctacactg gttccacttt
    30361 tgtgatttac acgggttacc catgaagctg accatcattc gattagaaaa ctttagcgac
    30421 caggaccgga ttgacctgca aaagatctgg ccggagtatt ccccttcctc tttacaggtt
    30481 gacgataacc accgtatcta cgccgcgcgt tttaacgagc gtctgcttgc ggccgtgcgg
    30541 gtaaccttaa gcggcaccga gggagcactg gattctctac gcgtgcggga agtcacccgc
    30601 cgtcgcggtg tggggcaata tctgctggaa gaggttttgc gtaacaatcc tggcgtttca
    30661 tgctggtgga tggcggatgc aggcgtggaa gatcgcggtg tgatgacggc gtttatgcag
    30721 gcactgggat ttacggcaca gcagggcggc tgggagaagc gttaatcgtc aagttgattt
    30781 caaaagtgat gaccaacgag aaattgcctg atgcgctacg cttatcaggc ctataatgtg
    30841 tgctgcaatt tactgatttc tttggttctt gtaggccgga taaggcgttt acgccgcatc
    30901 cggcatgaag caacgtattc gatattagca atttggcggc aacccaaagt tgccgattaa
    30961 caattacttc gcatcggtcg ccgtgccgtt ggcgtgccag tcaaatacgc cgaactcaaa
    31021 gccctttaga tcgcctttct catcccaggt cagtggcccc atcacggtat ccacggagtt
    31081 cgcttttagg tatttggcga tttcagccgg atcgtcagac tgattcaggc ccgcctgcaa
    31141 agattgcagc gcggcgtagg tggtccagac gaatgcgccg cttgggtcct gttttttcgc
    31201 tttgatcgca tcaacaatgg gtttgttcgc cggaacctga tcgtagttct tcggcttggt
    31261 caccagcagc ccttccgctg attcgcccgc aatgttagac agcgaaacgt tagccacacc
    31321 ttccggcccc ataaactgag ttttcagccc tgccgcgcgt gcctgacgca ggatttgccc
    31381 catttccggg tgataaccgc cgtagtaaac gaagtcgata ttctcttttt tcagacgcgc
    31441 caccagcgtt gagaaatctt tttccccggc ggtgatacca tcaaagaaca ccacgttagc
    31501 attgcctttc ttcaggccgt cctgcaccgc tcgtgccaga ccttcgccgt attgctgttt
    31561 gtcgtgaacg atagcaatac gctgcggttt cactttctca agaatatatt tcgccgccgt
    31621 cggcccctgg tcggagtcca ggccggtggt gcgcaggatc agctgatagc cacgggcggt
    31681 cagctccggc gcggttgccg ctggggtgat cattaaaatg ccttcgtctt cgtagatgtc
    31741 agacgcaggc tgcgttgatg aagaacagag gtgaccaatc acatatttaa tgccgtcgtt
    31801 aacgactttg ttcgccaccg caaccgcctg tttcgggtca caggcatcgt catattttac
    31861 gatttgcagt ttgttgcctt taatgccgcc tttagcgttg atatccgcaa ccgcctgctc
    31921 tgcgccggta aactcctggt caccgtactg cgcaaccgga ccggacattg cacccacgac
    31981 cgcgacttta atatcttctg ccagagccat attgctgaat gccagcgcga tacatcctgc
    32041 cagtaacgct ttacccttta tgttcatcct gagaatcccc attcttctgg ttattacgtg
    32101 tgttgtgatg ttgttgttca gcactttatt tcgttttatg catgactacc cgtgctttag
    32161 cagcatactc tgctaaaaca tacccgattt ttatgatatt ggaatagcta ttttgacagt
    32221 ttattaacaa tctgcgtggg gattggcgtt ttgccggagg gggaattgat tattgtagag
    32281 gcccaaaaaa caaaaacccc ggactctcat ccagggttct ctgcttaata gcggaaatta
    32341 cgcttcaata gcggcgcgaa gttttttcat cgcgttcttt tccagttggc gcacacgttc
    32401 agcggaaaca ccgtaacggt cagccagttc ctgcaacgtg gacttgttgt cttcgtccag
    32461 ccagcgcgca cggatgatgt cctggctgcg ttcgtccaga ccatgcatcg cgtcggtcag
    32521 acggtttgcc gcctgctctt cccagttatc atcttcgatg ccgtcggcaa agttagatga
    32581 tttatcctgc agatagagca ccggtgccat cggctggctg tcggaatcgt cgtcggaaga
    32641 caggtcaaag gtcatgtcct gtgccgccat acgtgattcc atctcacgta cgtctttgct
    32701 ggttacgccc agttcacggg ccaccatttc gacttcatcc tggttaaacc agcccagacg
    32761 ctgcttggtt ttacgcaggt tgaagaacag tttgcgctgc gctttggtgg tcgcaacttt
    32821 gacgatacgc cagttacgca gaacgtattc gtggatctct gctttgatcc agtggacggc
    32881 gaaggagacc aggcgcacac ccacttccgg gttaaaacgg cgcactgctt tcatcaggcc
    32941 gatgttacct tcctgaatca aatccgcctg tggcaggcca tagcccgcat aattacgagc
    33001 aatatgaaca acaaaccgca ggtgagacag gatcagcgtt ttagctgctt ccagatcgcc
    33061 atggtaatgc agcttttcag ccagcgcccg ctcctcgtca gccgacaaca tcggccacgc
    33121 gttagctgcc cggatgtagg aatccaggtt gccaactggg gctaaagcta aactttgcat
    33181 tttgtcagtc attcaaatcc tctcaatcga tatcttctgg cgcttcagtg gtagcaacaa
    33241 ctgtgccaaa gcttaagagc aacgaggtta tcattcactg ttttatcaga ccgtgatttt
    33301 atccacaagt tcaatgcaag cttgtgaata aattacgcac aaaatgtgac atagagatga
    33361 aataccggga agagacaacg gggtctcttt ccctgctacg gaacccattg cagggaaaga
    33421 gtataacacg cttttattat tcaggcgtaa agtggcgtaa atgttgtacc gtggcaagcc
    33481 acgctgccac ccagccaatc atcgagcata ccagcagcaa tagcaggcat tcatcgaacg
    33541 ataaaccatt gatatcaaac ttcgttccga aaacctgtgc cacttccgcc accgctgacg
    33601 acaaacgcag taccagaatt tctgacaaaa ttaatgacaa caatgcgcca gaaaatccca
    33661 gcagcgcacc accgtacagg aacgggcgaa ggatgaaacc atccgtcgca ccaatcagtt
    33721 tctgcacgtt gatggagtca cggcgagcaa agatgctcag acgtacgctg ttgccgatga
    33781 cgaggaacac tgccgccacc atcaacacgc cgatcatcgc cgaaacgcgc ccaaccagcc
    33841 cggtcaacgc cgccagacgg gcaaaccagc tgtcatccat ccgcacttcg ccaatgccgt
    33901 taatctgcgt gatacgatca cgcagcgtat tgagtgattc cgtcccctga aaatcgagtt
    33961 tcgggatcac caccgccact gccggaagcg ggttttcttc cagcatatcc agcgcaccac
    34021 caaaaccaga ccagttacgg aactcgccca gtgcgtcttc acgagaaaga tagttcactt
    34081 tctccacgcc ttgctcggcc tgcaactgtg ccaccacgcc cgcagcggcg tcatcgtcca
    34141 gcgttttttg caagtaaaca gtgatttgcg gtgacggata atactgcgtc gccgcctgat
    34201 taacgttttt gtacaccata taacagacgc tgggcagcgt cagggagatg gcgataacca
    34261 tcaccgttaa aaacgtggcg aacggtttgc ttttcagatc ctgcaatgcg ccgtggaagg
    34321 catagcgcac ctgttcgttg aaaacgttgg ttttgcgatt taccggtttt ggcgaggatt
    34381 tcgcgcgttt cggtgcgtta cgaccgccgt cgcctgagcc gccgaccgat ttacggaagc
    34441 gatccagacg cccaccaaac tgccgaatat gattgattgc atcgcgctta ttcatggccc
    34501 acgcctccat gcaagtgacc atcgctcagg gtgagcatgc gataggaacg ccgtgaaatc
    34561 aggttaatgt cgtgcgttgc catcaatacg gttaccccaa cgcggttaaa ctcttcaaac
    34621 agacgtagaa tcccttccga caacgcgtcg tccaggttac cggtcggttc gtccgccagc
    34681 agtaccgcgg gcttattcac caccgcgcgg gcaatgccaa cacgctgttg ttcaccgccg
    34741 gaaagctgaa tagggaagtt cttcgctttg tccagtagcc cgactttatc cagtgccgcc
    34801 gacacccggc gacgaatatc gtcaccgctg gcaccagcga taatcagcgg gatcgccacg
    34861 ttatcgtaga cagtacggtc catcagtaga tggtgatcct ggaaaatcat gccaatctgg
    34921 cggcgcagaa acggaacttc acggtttttc agacgcgtga tgtcatggcc gctaaaccag
    34981 attttcccgg cgctgggccg ctcaatccca cagatcagct tcaggagggt acttttccct
    35041 gcgccggaat gaccggtcag aaacgccatc tcacccggct gcatatggaa cgtaacgccc
    35101 tgcagcgcct gtctcccacc gagataagcc ttgctgacat gttcaaagcg aatcattgtt
    35161 aatcctctcg ggcaaaaagt gcctctataa agtcgtccgc cttaaacgga cgcaaatcct
    35221 caatacgttc gccgacacca atgtagcgga tagggatacc aaactggtca gccaccgaga
    35281 aaattacccc gcctttcgcc gtgccgtcca gtttcgttag cgtgatgccg gttaagccaa
    35341 cagcttcgtg gaacagtttg gcctggctta ccgcgttctg cccggtgctg gcatcaatag
    35401 tcagcataac ttcatgcggc gcttcaacgt cgagtttctt catcacgcgg acgattttct
    35461 tcaactcttc catcagatgc gatttgttct gcagacgtcc ggcggtatcg gcaatcagga
    35521 cgtcgatatt acgcgcttta gctgcctgaa tggcgtcgaa gataacagag gcggaatccg
    35581 caccggtatg ctgggcaatc accggaatat tgttgcgctg accccagacc tgaagctgtt
    35641 caaccgcagc tgcacggaaa gtatcgcccg ccgccagcat caccgattta ccctgctgct
    35701 caaactgacg cgccagctta ccaatcgtcg tggttttacc cacaccgttg acgcccacca
    35761 tcaggatcac gaacggcgtt ttatcttcaa cattcagcgg ctcatcgact ttcgccagaa
    35821 tctcacccat ctcttctttc agcaggccat agagcgcctc ggcatcacga agctgcttgc
    35881 gggatgcgcc ttccgtcaga ttggtgataa ttttacgtgt ggtttccacg cccacatcgg
    35941 cgatcaacag ctgttcttcc agctcttcaa acagatcatc gtcgattttt ttaccgcgga
    36001 acaggctgat aaatccggaa ccgagatttt ctttggtttt taacaggctg cgtttcaggc
    36061 gcgcgaagaa gccttctttg gtcggttttt cttgctcctg ggcaatttct tcaaccggct
    36121 gttcttcttc tgccggagga actaccatca ccgcctcttc tgccgcttcg gcagccagcg
    36181 cctgtgcttc cagctcttcg tcggtgattt cttctttagc cgcttcttct tctgccgctt
    36241 cgacaatctc tacggtttcc gcttcagcct gccactcttc tggcgaaacc tcttcggcgt
    36301 tgacgtcttc cggcaacggc agctcttcac gttcgatagc cactggctcc ggcgtttctt
    36361 ctacgaccgg ttccggctgt gcaacaactt ccgcttcagg ctgcgctttt tcactttcaa
    36421 caacctgttc agtgacttcc acaacgtcgg cagcaaaagt ttccgcctcg gcttcagtat
    36481 gcgcctgcgg ctgctcttca acggcttgtt cagaagcctt cgcaggctct tgcgcctgaa
    36541 cgatttcttc tacaaccggt tgttcattct gaacttctgt ctctttttcc ggggtctgct
    36601 ctttttgacc aaagcccagc caggaaaaaa agccacgtct tttttctttc gccatttgcg
    36661 actacactcc tcgctgttgc ttcatggcac agcgttaacg ctatgtacat agcagctaaa
    36721 aaaatgatga aatagtctat cacttaactt aattcacatc accgcctgca agtatgtgtt
    36781 atctggcgga ttgagcaatt tatcatgaaa aaaccgaatc attccggcag cggccaaatc
    36841 cgcattattg gcgggcagtg gcgaggccgt aaactcccgg ttcctgatag cccaggtctg
    36901 cgccccacca ccgaccgcgt acgcgaaacg ttgtttaact ggctggctcc ggtcattgtt
    36961 gacgcccaat gtctggattg cttcgccggg agcggcgcgc tggggctgga agcgttatcg
    37021 cgctacgctg cgggggcaac gttgattgag atggatcgcg tggtttctca gcagttaatt
    37081 aagaatctgg cgacactaaa agcaggcaat gcacgcgtgg tgaacagcaa cgcaatgtca
    37141 ttcctggcgc aaaaaggcac accgcataat atcgtgtttg tcgatccacc gttccgccgt
    37201 ggcttgttag aagagacgat aaatttactg gaagataacg gctggctggc tgacgaagcc
    37261 ctgatttatg tcgaaagcga agtcgaaaac ggtctgccca ctgttccggc aaactggtca
    37321 ttacatcggg aaaaagtggc gggtcaggtg gcttatcggc tgtatcaaca cgaagcacaa
    37381 ggagaaagtg atgctgatta atattggtcg tttgttaatg ctctgcgttt ggggattttt
    37441 aatcctcaat ctggtgcatc ccttcccgcg cccgctaaat attttcgtta acgtggcgct
    37501 gatttttacc gtgctgatgc atggtatgca gctggcgcta ttgaaatcca ctttaccgaa
    37561 agatggcccg cagatgacca ccgccgaaaa ggtgcggatt ttccttttcg gcgtgtttga
    37621 actgctggtc tggcagaaga aatttaaagt taaaaaataa ccttattgtt cgccaacaaa
    37681 gctaacaaag cgcgttcctt tatagctcag cgtaccttta tcgcccacgg tcagggcgtg
    37741 gtactgctgg gcgtcgagac gaaacgtctg ctccattcct ccgctttgcg gtttgaagct
    37801 tgcctcatag cgcatactgg tgcctgccgg agtcacttcc tgctggcgcg aacggcgatc
    37861 gttaatcggt ttttcccgct tgttgctcac caccaccagc ttttgctgga gcggagccat
    37921 atcattatca gctttttccc gtcgctgttg cataaaacga aacgatgcgg cgacgacaat
    37981 taagccaatg ataacaataa agaaaagagg tggtttactc atctttatcc ctcatcggaa
    38041 aatgcggaat taagcatacc ctgccagtta tggtgttgtc atccgtccac cctcgccact
    38101 aaactggaag caagaccgta ggcattccgc ttacgaaaaa ataacgaatt caaggaacta
    38161 agatgctttg gtcgtttatc gctgtctgtc tttccgcatg gctatctgtg gatgcatcgt
    38221 atcgtgggcc aacctggcaa cgctgggtgt ttaaaccgtt aacccttctt ctcctgctgt
    38281 tactggcctg gcaagcaccg atgttcgacg ccattagcta tctggtgctg gcgggactgt
    38341 gcgcctcact gctgggcgat gcgctaaccc tgttgccacg tcaacgtctg atgtacgcca
    38401 tcggcgcgtt tttcctctcg cacctgctgt acaccatcta tttcgccagt cagatgacgc
    38461 tctctttctt ctggcctctg ccactggtgt tgctggttct gggtgcgctg ctactggcga
    38521 ttatctggac gcggctggaa gagtaccgtt ggcctatctg cacgtttatc ggcatgacgc
    38581 tggtgatggt gtggctggca ggtgaactgt ggttcttccg tccgaccgct ccggcgctct
    38641 ctgcgtttgt tggcgcttcg ttgctgttta tcagtaactt tgtctggctg gggagccact
    38701 atcgccgacg cttccgtgcg gataacgcga ttgctgcggc ctgctacttt gccggtcact
    38761 tcctcatcgt ccgctcactg tatctctgat aaaacttgac tctggagtcg actccagagt
    38821 gtatccttcg gttaatgaga aaaaacttaa ccggaggatg ccatgtcgac acctgacaat
    38881 cacggcaaga aagcccctca atttgctgcg ttcaaaccga taaccacggt acagaacgcc
    38941 aacgactgtt gctgcgacgg cgcatgctcc agctcgccaa cgctctctga aaacgtctcc
    39001 ggcacccgct atagctggaa agtcagcggc atggactgcg ccgcctgtgc gcgcaaggta
    39061 gaaaatgccg tgcgccagct tgcaggcgtg aatcaggtgc aggtgttgtt cgccaccgaa
    39121 aaactggtgg ttgatgccga caatgacatc cgtgcccagg ttgaatctgc ggtgcaaaaa
    39181 gcgggctatt ccctgcgcga tgagcaggcc gccgacgagc cacaagcatc gcgcctgaaa
    39241 gagaatctgc cgctgattac gcttatcgtg atgatggcaa tcagctgggg tctggagcag
    39301 ttcaatcatc cgttcgggca actggcgttt atcgcgacca cgctagttgg gctgtacccg
    39361 attgctcatc aggcattacg gctgatcaaa tccggcagct acttcgccat tgaaacctta
    39421 atgagcgtag ccgctattgg tgcactgttt attggcgcaa cggctgaagc tgcgatggtg
    39481 ttgctgctgt ttttgattgg tgaacgactg gaaggctggg ccgccagccg cgcgcgtcag
    39541 ggcgttagcg cgttaatggc gctgaaacca gaaaccgcca cgcgcctgcg taacggtgag
    39601 cgggaagagg tggcgattaa cagcctgcgg cctggcgatg tgattgaagt cgccgcaggt
    39661 gggcgtttgc ctgccgacgg taaactgctc tcaccgtttg ccagttttga tgaaagcgcc
    39721 ctaaccggag aatccattcc ggtggagcgc gcgacgggcg ataaagttcc tgcaggagcc
    39781 accagcgtag accgtctggt aacgctggaa gtgctgtcag aaccgggtgc cagcgccatt
    39841 gaccggattc tgaaactgat cgaagaagcc gaagagcgcc gcgcccccat tgagcggttt
    39901 atcgaccgtt tcagccgtat ttatacgcca gcgattatgg ccgtcgccct gctggtaacg
    39961 ctagtgccgc cgctgctgtt tgccgccagc tggcaggagt ggatttataa aggtctgacg
    40021 ctgctgctga ttggttgccc gtgtgcgtta gttatctcaa cgcctgcggc gataacctcc
    40081 gggctggcag cagcagcgcg tcgtggggcg ttaattaaag gcggtgcggc gctggaacaa
    40141 ctaggtcgtg ttacccaagt ggcgtttgat aaaaccggta cgctgaccgt cggtaaaccg
    40201 cgcgttaccg cgattcatcc ggcaacgggt attagtgaat ctgaactgct gacactggcg
    40261 gcggcggtcg agcaaggcgc gacgcatcca ctggcgcaag ccatcgtacg cgaagcacag
    40321 gttgctgaac tcgccattcc caccgccgaa tcacagcggg cactggtcgg gtctggcatt
    40381 gaagcgcagg ttaatggtga gcgcgtgttg atatgcgctg ccgggaaaca tcccgctgat
    40441 gcatttgctg gtttgattaa tgaactggaa agcgccgggc aaacggttgt gctggtagta
    40501 cgtaatgatg acgtgctggg tatcattgca ttgcaggata ccctgcgcgc cgacgctaca
    40561 actgccatca gtgaactgaa cgcgctgggg gttaaaggag tgatcctcac cggcgataat
    40621 ccacgcgcag cggcggcaat tgccggggag ctggggctgg agtttaaagc gggcctattg
    40681 ccggaagata aagtcaaagc ggtgaccaag ctgaatcaac atgcgcccct ggcgatggtc
    40741 ggtgatggta ttaacgacgc gccagcgatg aaagctgccg ccatcgggat tgctatgggt
    40801 agcggcacag acgtggcgct ggaaaccgcc gacgcagcat taacccataa ccacctgcgc
    40861 ggcctggtgc aaatgattga actggcacgc gccactcacg ccaatatccg ccagaacatc
    40921 accattgcgc tgggactgaa agggatcttc ctcgtcacca cgcttttagg gatgaccggg
    40981 ttgtggctgg cagtgctggc agatacgggt gcgacggtgc tggtgacggc gaatgcgtta
    41041 aggttgttgc gcaggagata aggcaaaccg atcgcaacat tgagcgcgat cggtcccctc
    41101 gcccctctgg ggagagggtt agggggtgag gaaaaggcgg catcgaagcc aatcagcccc
    41161 tatcaaccgc ctttacgaat caaataacga taaggcagtc catccgtctc tttcgcaacc
    41221 agttcgtgtt ccataaaggt acaaaaccca ggaatatcgc gggtagtggc cggatcgtcg
    41281 gcgataatca gcaacgtttc gccaggctgc atattgcgca cggttttgcg caccatcatc
    41341 accggttccg ggcagcgcag gccaagcgcg tcgagtgtgt ggtcagggct ggaaaagaga
    41401 tcggtcattt tcttctcatc acttaaaaaa acggcgctag tttacgccct gtgagtccgt
    41461 aagcaaacca ggttaacgat tgcgtgaaaa ttaaccattg cattgtcaac gtaaagcagt
    41521 atcatgcggc ggctcgaaaa aagggtaagc acgttattat gttaaggtaa cagacgtgtc
    41581 gtacgtattg ggttccctca ccccaatggt taatcaaaaa ggtacaatat gaacgttttc
    41641 tcgcaaactc aacgctataa ggccttgttc tggttatcgt tatttcatct tctggtgatc
    41701 acctccagta actatctggt tcagcttccc gtctccattt ttggttttca taccacctgg
    41761 ggcgcgttta gctttccgtt tatttttctt gctaccgacc tgaccgtgcg tatttttggc
    41821 gcaccgctgg cccgacgcat tatcttcgcg gtaatgatcc ctgcgttatt aatctcctac
    41881 gtcatctcgt cgctattcta tatgggttcc tggcagggat tcggcgcact cgcccacttc
    41941 aacctgtttg tcgcccgtat cgccaccgcc agtttcatgg cctacgcgct ggggcaaatc
    42001 ctcgacgtgc acgtttttaa ccgcctgcgt cagagtcgcc gctggtggct ggcaccgaca
    42061 gcgtccacac tgttcggtaa cgtcagcgac acgctggcct ttttcttcat tgccttctgg
    42121 cgtagcccgg atgcctttat ggctgaacac tggatggaaa tcgcgctggt cgattactgt
    42181 ttcaaagtgt taatcagtat cgttttcttc ctgccaatgt atggcgtatt actcaatatg
    42241 ctgttgaaaa gactggcaga taaatccgaa atcaacgctt tgcaggcgag ttaaaggttc
    42301 gttatcagag ttgtgataag atggatgaat gagccgttat ggccgtttat cgaaaggaag
    42361 aagtcaatgc gcaatctggt taaatatgtc ggaattggcc tgctggttat ggggcttgcg
    42421 gcctgtgatg ataaagacac taacgctacg gcgcagggtt cggtcgcgga aagtaacgct
    42481 accgggaatc ccgtcaacct gcttgatggc aagttaagtt tctcgctgcc agcggatatg
    42541 accgaccaga gcggtaagct gggaacgcag gccaataaca tgcatgtctg gtccgacgcc
    42601 accgggcaga aagcagtcat cgtcatcatg ggcgatgatc cgaaagaaga tctggcggtg
    42661 ttggcgaagc gtctggaaga tcagcaacgt agccgcgatc cgcagctgca agtggtaacc
    42721 aataaagcca ttgagctgaa aggtcacaaa atgcagcagt tagacagtat tatctccgcg
    42781 aaaggccaga cggcgtactc ttccgttatt ctgggtaacg tgggtaatca actgctgacc
    42841 atgcaaatta cgctgccagc tgacgatcag caaaaagcgc aaaccaccgc agaaaacatc
    42901 attaatacgc tggttattca gtaagtttta agatgatgag gcggcctcag ggacgtgttc
    42961 cggaggccgt ttttttaatc gccacgtcag taataacgcg attgcgacca gtcccgccgc
    43021 cgccagataa atcaccggta cgcccgccca gctcatcacc agcccagcca gtggtccagt
    43081 cacgccaagc gataaatcca taaatacggt gtaagttgcc agcgccgccc cctaattttg
    43141 ctgcggaacc gcttttaccg ccactacacc caatgccggg aacaccagcg aaaacccggc
    43201 ccccgccagt aagacgccga ttttcgccat ccacggcata gtcgccacgc caaccagtag
    43261 caggccgatt atctcaacgc taaagcaaat catcgctacg tttaagccac cgatacggtt
    43321 aatgccgtta gggaataaca aacgcgtacc gacaaacgca cagctaaaca gcgtcagcgc
    43381 gaaagccgca ccgtcccaac ctttagcgtc ataaaacagc gtgataaagg tggcgatgac
    43441 gccaaatccg gcggaagcca gtgccagcgc cataccgtac agccagacgc gcccaagcac
    43501 cgcgcgaaac ggcagcggtt tgcctttact ggcttttacc gtcggacgcg ggatcgccaa
    43561 caaaatggcc accagcgcca cgcccataat gattaacgct aacgcctgca agccgcccca
    43621 gtgataaaac acgacgccta acggcgcacc catcgccatc gccccgtaag tgacaatgcc
    43681 gttccacgaa atcacccgcc cgatatgcag cgagccaacc actccaacgc cccatagggt
    43741 cgatcccgtt ccggcaaaac tttgcccaat cccaaggatg acgcgcccca ggcaaagtaa
    43801 taacaggctg atgacaggca gactggcggt taatcctgcc gtcagatacc ccagaccgct
    43861 caaaaagcag ccgcataaac cgaagacgac aatctttttg ggtcccagca aatcggcgta
    43921 acgtccggca tgagggcggc tcagcaaggt ggcgaaatat tgcaggctga taaccaatcc
    43981 tgcccagaag gcgctaaagc ccatcacatc atggacatag cccggtaata cagcgagcgg
    44041 caacccgatg gtgaggtagc tggcgaagtt aaacatgaca atggagacaa tgcgcaaatt
    44101 caggcgcaat ccgtttagcg cgggttcggc tacgggttcg ggcatgagga tcaccacatt
    44161 tttacaacag tgtttcattt ttaccatgtg ctgacgtgaa aatcagcagt aagaatcaga
    44221 atattgctgg cgtgactccc gctacactta acacaaaaag tcacaaggaa gccccaatgg
    44281 aaacccctca acccgataaa acgggcatgc acattctgct caagttggcc tcgctggtag
    44341 tgatcctcgc gggcattcac gcagcggcag atatcattgt gcagctgtta ctggcgctgt
    44401 tttttgccat cgtcctcaac ccgctcgtta cctggtttat tcgtcgggga gtacaacgcc
    44461 ccgttgccat tacgattgta gtggtggtga tgctgatcgc actaaccgcg ctggtcggcg
    44521 tactggcggc atcgtttaac gaatttatct ctatgctgcc gaagtttaat aaggagctga
    44581 cgcgcaaact ttttaaattg caggagatgt tgccttttct taatttgcat atgtcgccgg
    44641 agcgaatgct gcagcggatg gactcggaaa aagtggttac cttcaccaca gcgctaatga
    44701 ccgggctttc cggggcaatg gcgagcgtgc ttttgctggt gatgaccgta gtttttatgc
    44761 tgtttgaagt gcgccacgtc ccttacaaaa tgcgttttgc gctgaataac ccacagattc
    44821 acatcgcagg attacaccgc gcacttaaag gcgtttcgca ttatctggcg ttaaagacgc
    44881 tactcagttt atggacaggt gtcatcgtct ggctggggct ggcgctaatg ggcgtacagt
    44941 ttgcgctgat gtgggcagta ctggcgtttt tgctcaacta cgtgcccaat atcggcgcgg
    45001 taatttccgc cgtaccgcca atgattcagg tgctgctgtt taatggtgtt tacgaatgta
    45061 ttctggtcgg cgcattgttt ttagtggtcc atatggtcat cggcaatatt ttagaaccac
    45121 ggatgatggg ccatcgcctg gggatgtcca ccatggtggt atttctttca ttgttaatct
    45181 ggggatggct gctcggcccg gtagggatgc tactttcggt gccattaacc agagtgtgta
    45241 aaatctggat ggaaacgacg aaaggtggca gcaaactggc gattttactg gggccggaca
    45301 gaccgaaaag tcggttaccg ggatgaggcg acaagtgata cgatacgcac tttcattttc
    45361 cattaaacgt tggccctgat atgtatcgga tagttctggg gaaagtttcg accttaagcg
    45421 cagctccact gccaccgggt ttacgcgagc aagcaccgca aggtccacga cgcgaacgct
    45481 ggctggcggg gcgtgcattg ctttcgcaca cgctttcccc gctaccggag atcatctatg
    45541 gagaacaagg caaacctgca ttcgcgccgg aaacgccgct atggttcaac ttaagccata
    45601 gcggtgacga tatcgctctg ctgttgagcg atgaaggcga agtcggctgc gatatcgaag
    45661 tgattcgccc gcgcgccaac tggcgctggc tggcgaacgc cgtgttcagc ctcggggaac
    45721 acgctgaaat ggacgccgtg catcctgatc agcaactgga aatgttctgg cgcatctgga
    45781 cgcgcaaaga agccatcgtt aaacagcgtg gcggcagcgc ctggcaaatc gtcagcgtag
    45841 atagcaccta tcactcctcg ctgtcagtca gccattgtca gctagaaaat ttaagccttg
    45901 cgatctgcac ccccaccccc tttacgctca ccgccgacag tgtgcaatgg atcgattcag
    45961 ttaactgatc cgcccacccg actgcccatc tattgatcca gaacaggtaa tcagtatgac
    46021 gaatacttaa aatcgtcata cttatttccg ccatctattt taatccattg gggttaccat
    46081 gctctccaca ctccgccgca ctctatttgc gctgctggct tgtgcgtctt ttatcgtcca
    46141 tgccgctgca ccagatgaaa tcaccaccgc ctggccggtg aatgtcgggc cactaaaccc
    46201 gcacctttac acgcctaacc agatgttcgc ccagagcatg gtttatgaac cattggtgaa
    46261 atatcaggca gacggttcgg tgatcccgtg gctggcaaaa agctggactc attcagaaga
    46321 tggtaaaacc tggaccttca ccctgcgtga tgacgtgaaa ttctccaacg gtgaaccgtt
    46381 cgatgccgag gcggcggcag aaaacttccg cgcagtgctc gataaccgtc aacgtcacgc
    46441 ctggctggag ctggcaaacc agattgttga tgttaaagca ctcaataaaa cagagctgca
    46501 aattaccctg aaaagcgcct actatccttt cctgcaagaa ctggccctgc cccgtccttt
    46561 ccgttttatc gctccctcgc agtttaaaaa ccatgaaacc atgaacggaa ttaaagcgcc
    46621 gattggcacc ggaccgtgga ttttgcagga atcgaaactg aatcagtacg atgtcttcgt
    46681 ccgtaacgaa aactactggg gcgaaaagcc agcgattaaa aagatcacct ttaacgtcat
    46741 cccggacccg actacccgcg cggtggcgtt tgaaactggc gatatcgacc tgctgtacgg
    46801 aaacgaaggg ttattaccgc tcgatacctt cgcccgcttt agccagaatc cggcttacca
    46861 cacccaactg tcacagccgc tcgaaaccgt gatgctggcg ctcaataccg ccaaagcccc
    46921 caccaacgag ctggcagtac gtgaagctct taattacgcg gtaaacaaaa aatcgctgat
    46981 tgataacgcg ttatatggca cccagcaggt cgccgacacc ctgtttgccc cttctgtgcc
    47041 ttacgctaat ctcggcctga aaccgcgcca gtacgatccg cagaaagcga aagagttgct
    47101 ggaaaaagcc ggttggacgc tgcctgcggg caaagacatc cgcgagaaaa atggtcagcc
    47161 gctgcgcatt gaactgtcgt tcatcggcac cgatgcgtta agcaaatcga tggcggaaat
    47221 cattcaggct gatatgcgcc agattggcgc agatgtctcg ctgattggcg aagaagagag
    47281 cagtatctat gctcgtcagc gcgacggtcg ttttggcatg attttccacc gcacctgggg
    47341 cgcgccatac gatccacacg ccttcctgag ttcaatgcgc gtaccgtcgc acgctgactt
    47401 ccaggcacag caaggattag ccgacaagcc gctgattgat aaagagatcg gcgaagtgct
    47461 ggcgacccat gacgaaacgc aacgtcaggc gctgtatcgc gacattctga cccgtctgca
    47521 tgacgaggcg gtttatctgc ctatcagcta catctcgatg atggtggtat caaaaccgga
    47581 gctgggtaat atcccctacg cgccgatcgc caccgaaatt ccgttcgaac agattaaacc
    47641 ggtgaaacct taatgttgcg ttacgtatta cgccgctttc tgctgctgat cccgatggtg
    47701 ctcgccgcct cggtgatcat ttttctgatg ctgcgcctcg gtaccggcga cccggcgctc
    47761 gattatctgc gtttatctaa cctgccgccg acgccggaga tgctggcctc tacccgcacc
    47821 atgctcgggc tggatcagcc gctgtacgtc cagtacggta cctggttgtg gaaggcgctg
    47881 catcttgact ttggtatctc attcgccagc caacgcccgg tactggacga tatgctgaac
    47941 ttcctgccca ccacgctgga acttgcaggt gcggcgctgg tattaattct gctcacttcc
    48001 gtaccgctcg gtatctgggc ggcgcgccac cgcgatcgtt tgccggattt cgccgtgcgc
    48061 tttatcgcgt ttcttggcgt gtcgatgccc aacttctggc tggcattttt gctagtgatg
    48121 gcgttttcgg tgtatctgca atggctaccc gcgatgggct acggcagctg gcagcacatc
    48181 attttacctg cggtttccat cgcctttatg tcgctggcga ttaacgcgcg actgctgcgc
    48241 gccagtatgc tggacgtcgc cggtcagcgt cacgtcacct gggcgcggct acgcggcctg
    48301 aatgacaaac agaccgaacg ccgccatatt ctgcgcaatg cctcgctgcc gatgatcacc
    48361 gccgtgggga tgcacattgg cgaattgatt ggcgggacga tgattatcga aaacatcttt
    48421 gcctggcctg gcgtcgggcg ctatgcggtg tcggcgattt ttaaccgtga ctatccggtg
    48481 attcagtgct ttacgctgat gatggtggtg gtttttgtgg tctgtaattt gattgtcgat
    48541 ttgctcaacg ccgcgctgga cccgcgcatt cgtcgtcatg aaggagcgca cgcgtgaact
    48601 ttttcctctc ttcccgctgg tcggtacgcc tggcgctgat cattatcgcc ctgctggcgc
    48661 tgattgcgct caccagccag tggtggctgc cgtacgatcc acaggcaatt gatttgccgt
    48721 cgtgcctgct ttcgccggat gcgcagcact ggctgggcac cgatcactta ggtcgcgata
    48781 ttttctcgcg gctgatggca gcgacccgcg tgtcgctcgg ttcggtaatg gcctgcctgc
    48841 tgctggtgct gacattaggg ctggttattg gcggcagcgc cgggttgatt ggcgggcgcg
    48901 ttgatcaggc caccatgcgc gtcgccgata tgtttatgac cttcccgacc tcgattctgt
    48961 cgttctttat ggttggcgtg ctcggcaccg ggctgaccaa cgtaattatc gccatcgccc
    49021 tgtcgcactg ggcgtggtat gcacgcatgg tgcgcagctt ggtgatttca ctacgccaac
    49081 gcgagtttgt gctggcgtca cggctttccg gtgcgggcca tgtgcgggtg tttgtcgatc
    49141 atctggcagg cgcggtgatc ccttcgctgc tggtgctggc aacgctggat atcggccata
    49201 tgatgctgca cgtcgcgggg atgtctttcc ttggcctcgg tgtgaccgcg ccgaccgccg
    49261 aatggggcgt gatgattaac gacgcgcgcc agtatatctg gacccagccg ctgcaaatgt
    49321 tctggccggg gctggcgctg tttatcagcg tgatggcctt taacctggtg ggtgacgcac
    49381 tgcgcgatca tctggatcct catctggtga cggagcacgc acactaatgc cgcaacagat
    49441 tgaactacgt aatatcgcgc tacaggccgc gcagccgctg gtacacggtg tatcgttaac
    49501 cctgcaacgc gggcgcgtgc tggcgttagt cggcggtagc ggcagcggga aatcattaac
    49561 ctgcgccgcg acgctgggca ttttgcccgc tggcgttcgc cagacggcgg gggaaatttt
    49621 agccgatggc aaaccggttt cgccttacgc cctgcgcggc atcaaaattg ccaccatcat
    49681 gcagaacccg cgcagcgcct ttaatccact gcacaccatg cacacccacg cgcgggaaac
    49741 ctgcctggcg ttagggaaac ccgccgatga cgctacgctt accgctgcca tagaagcggt
    49801 ggggctggaa aacgccgcgc gcgtgctgaa gctgtacccg ttcgagatga gcggcggcat
    49861 gttgcagcgc atgatgattg cgatggcggt gctgtgtgaa tcaccgttta tcatcgccga
    49921 tgaaccgacc accgacctcg acgtggtagc acaggcgcgc atcctcgatc tgctggaaag
    49981 cattatgcaa aaacaagcgc cgggaatgct gctggtcacc catgatatgg gcgttgtggc
    50041 gcgtctggcg gatgacgtgg cggtgatgtc acaaggtaaa attgtcgaac agggcgatgt
    50101 agaaacgctg tttaacgccc ccaaacatac ggtgacgcgc agcctggttt ccgctcatct
    50161 cgccctctac ggtatggagc tggcatcatg accttactta acgtctgcgg cctttcccat
    50221 cactatgccc acggtggatt tagcggaaaa catcaacatc aggcggtgct gaataacgtt
    50281 tccctgaccc tgaaaagcgg cgaaaccgtc gccctgctgg gacgcagcgg ctgcggaaaa
    50341 agcaccctcg cgcggctgct ggtaggctta gagtctccct cacaagggaa tattagctgg
    50401 cgcggcgaat cgctggcgaa acttaatcgc gcccaacgta aagccttccg ccgtgatatt
    50461 cagatggtgt ttcaggactc catcagtgcc gtgaatccgc gcaaaaccgt gcgcgagatc
    50521 ctgcgtgaac cgatgcgcca cctgctatca ctgaaaaaat ccgaacaact ggcgcgcgcc
    50581 agcgaaatgc tgaaggcggt cgatctcgat gacagcgttc tcgacaaacg cccaccgcag
    50641 ttaagcggcg gccagctcca gctcgtctgc ctggcacgcg cgctggcggt cgaaccgaaa
    50701 ttgctgattc tggatgaagc cgtttctaac cttgatctcg tgttacaggc gggcgtcatt
    50761 cgcctgctga aaaatctaca acaacagttt ggcactgcct gcctgttcat cactcatgac
    50821 ttacgcctgg tggaacgctt ttgccagcga gtgatggtga tggacaacgg acaaatcgcc
    50881 gaaacccagg cggtgggaga taaattaacc ttttcctctg acgccggacg tgtgctacaa
    50941 aacgcggtat tacccgcatt ccccgtgcgc cgtcgcacct cagaaaaggt ttaacgccaa
    51001 tgcaacgagt caccatcacg cttgatgacg atttactgga gacgctggac agcctgagcc
    51061 agcgtcgtgg ttataacaac cgttccgaag ctatccgcga tattctgcgt agtgccctgg
    51121 cgcaggaagc tacccagcag cacggcacac aaggtttcgc ggtgctgtcg tatgtgtatg
    51181 aacacgaaaa acgcgactta gccagccgta ttgtttccac ccagcatcat caccacgacc
    51241 tctccgtcgc cacgctacat gtgcacatca accacgacga ctgtctggaa atcgccgtgt
    51301 tgaaaggtga catgggtgac gtgcagcatt ttgccgatga cgttatcgcc cagcgcggcg
    51361 tgcggcacgg gcatttgcag tgcctgccga aggaagattg agttatttct aaaacaagga
    51421 aagaaaagat attaactgaa cttaatttat aaaattaagt tcagttgcat agatagatcc
    51481 tgtaaaggca gatattctgc gtaatgtacg tcgctaaaat ttataaatga ttcaaacgct
    51541 cattcagtgt ttcaataata taggtattaa cagagacttg ttgctgcgca gccgcattat
    51601 taaggcgttc actcagcgat tctggatagc gtaacgtaaa ggtcttaatc ttttctgtac
    51661 gcgcatacgg ctcaataccc gcagctttac aatcctcaag atactctcgc agggaaagct
    51721 ctccttcttt ttgcagcccc tgaatactgt cagagacaaa atcacaataa cctgacaagc
    51781 ccaggaattt gccacgaaat gcattcagtt ctggcacgta ggtaataaca gctggttgcc
    51841 cggcaatctc catagagttt ggcgtcttca gtttgatcat ggttttaccc ctatacttag
    51901 taaccagtca cgcacgcttt ctacagcgcc tttatcggta tctggcgacg gatgaggccg
    51961 atggaaacac gcaacgctca tatttagtat gaacttgcaa cgcgaacctc ttccttcctt
    52021 aatttctccg cctaatgctt taaccagtga ctctatatca gaccacttga tcccttgagg
    52081 aacaggtgtt ttaaatattt gatccaatgt atttttttgt ttcttccgta aagcctgtac
    52141 cttggttctc atggcatgtc cttatgactt cactttatga catcacagta tgaagtcaag
    52201 tttcccttcc ttacgccatt gtcccaatcg tcttcctgaa tcgcagcagt gcgatggtga
    52261 aaaacgcgcc gccaattgcc atcagcgtca ggaactgcgg ccagacgatt tcgaatccgg
    52321 caccccggta gaggatggcc tgcgcgaggc taacaaagtg tgtcgtcggc atggtcagca
    52381 taatgtcctg caccatctgc ggcatacttt cgcgcggcgt ggatccaccg gaaagcattt
    52441 gcagcggcag cagcaccaga atcaccagca gccccagttg cggcattgaa cgcgctatcg
    52501 tccccataaa aatgccgatt gacgtggtgg caaacagact gagcgccacg cccagcataa
    52561 acagcgggat cgagccttca atcggtacgc ccagtacacc tttcaccatc agcaccagcg
    52621 ataatcccga taccaccagc accaccagcc ccatcgacca gatcttcgcc atcatgatct
    52681 caaacggcgt tatcggcatc accagtaaat gttccaccgt gccgtgttca cgctcgcgga
    52741 tcagcgccga tccggtcaat acaatcgcca gcatggtaat gttgttgatg atcgccatca
    52801 ccccgccaaa ccacgcggga tcaaggttcg ggttaaagcg catccgggtt tccagcgata
    52861 ccaacggttc gctgttatca cggtagcgcg cgacaaagct gttcacttca ccgttgataa
    52921 tattctggat atacccattg ccggtaaatg cctggctcat gcgcgtggca tcgacgttca
    52981 cctgaatatc cggctggcgt ccggcgagga catcacgctg aaaattaggc ggaatattta
    53041 tcgcgaaggt atagcgtccg gcgtccagtc cggcatccat ctcatcggcg gtgatcatct
    53101 ccggtggcaa aaaccacgga cgatagaagc tgttaacgat ccggttcgat aactgcgatt
    53161 gatccatatc ggcaatggcg atcggcgcga ggttcaacga tcctggcgta acggtcgctg
    53221 acgaatacac cgacaccgta aacgagaaga caatcagcgt cagcatcgct ttatcaccga
    53281 gcagactgcg caactctttg atacccagat taaaaatatt gcgtaaatgg cgcatcatcc
    53341 ctcctgtttt ttcagcagca ggatgcttaa gcccatcacc agcgggatgg ctatcagcaa
    53401 cgggataaaa agttgccaca aatcagtcag atccagcgct ttcgagaacg ttccgcgggc
    53461 gatagtcaga aaatgactgg tcgggtaaac ctcgccgatc caacgtccag gcccttccag
    53521 cgaagctacc ggatcgatca tcccggaaaa ctgtgtcgcc gggatcaatg tgataatcgc
    53581 cgttccgaaa atggcggcaa tctggctttt cataaaggtg gagatcagca gccccattcc
    53641 agtggcaatg atgatataca gcagcgccgc caaggtgagc gtcaggaaac tgcctttatg
    53701 cggtacgcca aacacaaaca ccgacaggcc gcagagcagg aaaaagttca gcatccccag
    53761 cgcgatgtac ggcagctgtt tgccaagcaa aaattcgcta cgggtggtgg gggtcacgta
    53821 aaggttgata atcgacccta gctctttttc ccgcaccacg ctaagggcgc ttagcattga
    53881 cgggatcatc atcagcagaa gcgggatcac cgccggaaca atcgctggca ggctttttac
    53941 gtccgggtta tagcgataac gcgtctcaat gttcatcagc ccgctttggc tggcgggtgt
    54001 caattgtcgg ctcgccacat cctgaagcca actctggtgc attgcctgca cgtaaccttt
    54061 taccgtttcg gcacgactgg gcatcgctcc gtcgatccag acgccgagtt ccacaggcgt
    54121 accacgcgcg atatcgcgcc cgaaattggg cgggatctca atggccaccg tgatatcgcc
    54181 cgcacgcatc cgacgatcaa gctcgtcata actggtgagc ggcggctgtt cgataaagta
    54241 acgggaaccg gagaggttga gtgtccacgc ctggctactg acggtctggt cgcggtcgag
    54301 caccgcaaag cgcaggtttt ccacatccat actgatgccg taacccatta tcagcatcag
    54361 gatcaccgtt cccatcagcg ccagcgtcga acgtactgga tcgcgtcgca gttccagcgc
    54421 ttcgcggcgg ctgtagctaa acagacggcg caggctaaat ccctgacgcg gcgcgtgggt
    54481 ggtgtcgtgt accacgggcg gcgcttcggc ttcgttgctc tgccctgccg cttcctgcaa
    54541 ataggcgata aatgcctctt ccagactggc ggctccgcgt ttctcaacca gttcctgcgg
    54601 tgtaccgctg gcaagcactt ttccggcgtg catcagtgag atgcggtcgc aacgttccgc
    54661 ttcgttcata aagtgggtgg agatgaagat agtcactttg tcctggcgcg agagatcgac
    54721 catcaactgc cagaacatat ccctcgccac cggatcgaca ccagaagtag gctcatcgag
    54781 gattaacatc tccgggcgat gaatcaccgc caccgccagc gaaagccgct ggcgaatgcc
    54841 gagcggcaat gactccggca gaacatcttc aacgtcgttg agcttaaaac gctcgctcat
    54901 ttcagccact cgcgcgggaa tttccgcttc cgggatgtga aacaaacggg catgtaactc
    54961 caggttttgc cgcacggtga gttcgttata gagcgaaaac gactgcgaca tatagcccac
    55021 acgacggcgg gtatcgatat cttttggatc aaccggttgc ccaaatagcc acgcctcacc
    55081 ttcgctggcg ggcagcagtc cggtaagcat tttcatggtg gtggatttac cgcagccgtt
    55141 cgaaccaagg aatccaaaaa tctccccgcg tggaatgcgg aaattgacgt gatcaacggc
    55201 aacgaaggaa ccaaaacgca tggtcagatc gcgcgcttcg atggcaatct ctgcgttttc
    55261 aggttgatac ggtgggatca ctaccgcctg atgcgcctgg cgttgcgctt gcggtaacag
    55321 attaataaat gcttcttcca gcgtggcgct ttgcgtttgc tgccgcagct cttcggcgct
    55381 gccggttgcc agcacttctc cggcattcat cgctaccagc cagtcgaagc gttcggcctc
    55441 ttccatatag gcggtggcga ccagcacgct catattgctc tgccgctggc gaatactgtc
    55501 gatcagatcc cagaactggg cgcgggagag cgggtcaacc cccgttgttg gctcatcaag
    55561 gatcaacagt tccgggtcgt ggattaacgc gcagcacagc ccaagttttt gcttcatccc
    55621 gccggagagt ttacctgccg gacgatcgcg aaacggtgct aacccggtgc tggtcagcag
    55681 ctcattaatt cgcacttccc gctccgcttt atcgtgaccg aacaggcgag cgaaaaaatc
    55741 gacattttca tacaccgaca aggtgtggta gaggtttttg cccagcccct gcggcatcca
    55801 ggcgatgcgc gggcagacgt ctcggcgatg cttcgggtcg tgcatatcgc cacccagcac
    55861 caccacattg ccctgctcaa tgacgcgggc accggaaatc agtgacaata aactcgactt
    55921 cccgacgcca tccggaccaa tcagcccgac catgcaacgg gccggaatat cgagagtgat
    55981 attgttcagc gcaacggttt ttccataatg ctggctcacg cccgccagtt gcgcgacagg
    56041 cgggacagga accagttcca gatgcgtcat tgcggcaacc tcaccacgag gtcgtcaggc
    56101 cacggaagtt cttcattcac ccgcacccac gctacgcccg gtaaaccggt tttgacatat
    56161 tccagatgct gctggagtaa ttccggtggg atacgcgctt tgacgcggaa catcagtttc
    56221 agccgttcat cgctggtttc gacggttttt ggcgtgaact gggcgacact ggcgacaaaa
    56281 ctgatggttg caggaatacg cagatctggc gcggcatcga ggatcagccg ggcttcaccg
    56341 cccagtttca gcgtgcccgc ctgttcggtt ggcaggaaga aggtcatata gacgtcgctg
    56401 agatcgacca tattcagcac ccgaccgcct gccgccagca cttcccctgg ctcggcaacc
    56461 cgatactgca cgcgtccgtc acgcggggct ttcagttcac tgtcatcgat atctgcggca
    56521 atgcgccgtt cagtggcttg cgctgcttcg acccgagttt gcgcctgaat gatattggtg
    56581 cgtgctgctt ctatagccgc tttagaagcc gaaacctggg ctttcgctga ttccagcgca
    56641 gctcgggcgc tctcagcggc ggcgcggtcg tcatccagct gttgcgcaga aatcgcccct
    56701 cgttgggcga gcgagcggga acgcgtatga cgtttggcaa cagagtccag ttcggcctgg
    56761 cgttgattga ccagcgactg tgcggcacga gtttcacttt gtcgttgctc cagcaaagcc
    56821 tgcgcggcgg caacggcgct ttgtgcctct ttgatttgcg cgatggcttc cagtcgctgt
    56881 tcctgcaaca cgcgagtatc catcttcgcc agcacttcgc cttcgcgaac aaactggcct
    56941 tctttcacca gaatggtgtc gatacgcccg gcagttttgc tggcgatatc cacttccgtc
    57001 gcttcaatgc gcccattgct gacagcaaag ccttccggca cacccgccgg gcgcaacagc
    57061 caccacgcca cgacggcagc caccgccagt gccccgacaa cccaccacgc cagatggcgc
    57121 ttactcttat ccataatcga cccgccataa tccctgtaag caaacgacgc gtgttacaac
    57181 tgccgccgaa cgacgttcag cggattcttc catccgggat aagaaggaaa cgacaaatac
    57241 gaccagcgcg cagagcattg agcgccaacg gggaaggctt agcgaaaacg ggtagtaatg
    57301 ttgtcatcat cagcattcct ggccgtaaat gaaacacgca tactcataag tgtgatctat
    57361 aaaactggct gacaaaacgg tatccatcct gttggatcgc gtttcacgcc tgttcagttg
    57421 caggaacaga gcattccttt ttacgtttta tcaacagtct caaccagata gtaacattaa
    57481 tattcatgtt gtctatggtt cagagctgta acattgtgcg ccttctaatc aaaggcataa
    57541 gtccatttct gtttttttca tgctggtaag acttccaggg agtggttatc tctggccctg
    57601 caccaaggtt aatttggcaa ttatgcatgc tgaacattct tccagcatgt ttactgtcac
    57661 gggcgttaag acctaaaact gtccccgttt cacctatgcg ttctctccca ccatttccgt
    57721 tatgtgcaac aacttgattt atacctaaca tttgtgaaca tgctaatagt gcttcaagtt
    57781 taggtctgta atctgtcgaa acagaaaaat aacgattaaa ggatcgacaa gagatagccc
    57841 ggctagtttt aaactttttg ccattaatta tagcatgatg ctttttatta ataatttccg
    57901 ctaattcgac cggatcgaga gcattggtca tttcatcaat agagacagtt atcggtccta
    57961 taatgtaacg ttttcgctga tcatcaaaag ttatcccatg atgatttgcc attattttgt
    58021 atttggggtc atagaaacat acaggataat cttttatccc gtcgtaggta tcctcatcaa
    58081 gtggtttcat gcgcgtgaag ttttgcatat agttaccatt gcaattcgtt tcatgatttc
    58141 cagctaatac atgaacattt tcattgatac tcattatgcg ttttaacaaa tcgatgataa
    58201 attgaacgcc acagatatta ctaaatcgat cgccggtttg gtcgccaaga aacgtacatg
    58261 gcgtgactgc gtctttatta ataaccaaat aaggctgaag actttctaaa acattatctc
    58321 ttctgttcag tatactgatt attgtattaa cattagcgtc aagttgctca ttaaagttct
    58381 cccttagcaa tacgttgaga acttcatctt ctatattaag caactggcta aggaaatttt
    58441 ctccttcagc catgattccc aaatgtcctg ttgcaaaaag cgcgtacaaa actgcaccga
    58501 ctgagctatc ggtatcgcca aaataggtcg gcctggtact tgtgggatca acaggtgaac
    58561 aattggagaa ataggttctg tcagcgaact ggggttttat aattgtttcg aatttaatat
    58621 caaatacatt gtattgcgat atttcccagt tccagatggg tttgtacgac tcagtaactg
    58681 caacatcatt agcattatca tttactgtca tattattaag taaataaaca atgctgcacc
    58741 aattaatatc ggtattatca agagggcaac cgtgattttt actattaatt gtcacaataa
    58801 actgataaca gttattccta cgtagaatat gagcgcgcat gcccgatagc atactatatt
    58861 tttctgaatg attcgtttgt atggtaaatg aaattttgtc atcttgaata gattgttgga
    58921 aacgttctat ttgccaatca tcaatttttt ccacaacttc accgagcatt gtcctggcaa
    58981 tacttgatat atcatcacct ataccatttg caatataccc aaacagttct ttagtgaagt
    59041 tggtaacatg ctcttgcacc atatcatttg ttgcgattgt cgttgtactg ccattagtgc
    59101 ctgccacagt tccaattttc atcttttacc tcatattatt caccgcgagg aatacatagt
    59161 taacatttta tcttatgaca tacacctcat attcgatggg aaaataatta tattttgcac
    59221 aaatcattca gccttaaagc ataatatgaa atttcttatc tcatagcaga gaaagtccct
    59281 aaagattgta gaggcgtcat cagtacatac ctctacaatc gattaagaaa acctatgaaa
    59341 tactggcaaa attaaaaaga gataccacag cactcatgaa gttttattga ggttgaaaac
    59401 aactaagccc tgggacacct tttttatcac cacgttcgtt aggtgctatc ttccaagaat
    59461 ttagccccat ggtataatta tcaatttttt gatgctggtc tacccctaat ccataatggc
    59521 caa
//
LOCUS       DAJTYG010000013        58365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTYG010000013 DAJTYG010000000
VERSION     DAJTYG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.013"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            545..1834
                     /gene="sdaC"
                     /locus_tag="OO568_000868"
     CDS             545..1834
                     /gene="sdaC"
                     /locus_tag="OO568_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6640332.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLSMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1892..3259
                     /gene="sdaB"
                     /locus_tag="OO568_000869"
     CDS             1892..3259
                     /gene="sdaB"
                     /locus_tag="OO568_000869"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6640333.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDISIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3370..4125
                     /gene="xni"
                     /locus_tag="OO568_000870"
     CDS             3370..4125
                     /gene="xni"
                     /locus_tag="OO568_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6640334.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTLAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4178..5326)
                     /gene="fucO"
                     /locus_tag="OO568_000871"
     CDS             complement(4178..5326)
                     /gene="fucO"
                     /locus_tag="OO568_000871"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6640335.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5354..6001)
                     /gene="fucA"
                     /locus_tag="OO568_000872"
     CDS             complement(5354..6001)
                     /gene="fucA"
                     /locus_tag="OO568_000872"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6640336.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6548..7864
                     /gene="fucP"
                     /locus_tag="OO568_000873"
     CDS             6548..7864
                     /gene="fucP"
                     /locus_tag="OO568_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6640337.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRTYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRITGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7897..9672
                     /gene="fucI"
                     /locus_tag="OO568_000874"
                     /pseudo
     CDS             7897..9672
                     /gene="fucI"
                     /locus_tag="OO568_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            9781..11200
                     /gene="fucK"
                     /locus_tag="OO568_000875"
                     /pseudo
     CDS             9781..11200
                     /gene="fucK"
                     /locus_tag="OO568_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11202..11624
                     /gene="fucU"
                     /locus_tag="OO568_000876"
     CDS             11202..11624
                     /gene="fucU"
                     /locus_tag="OO568_000876"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6640338.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11682..12413
                     /gene="fucR"
                     /locus_tag="OO568_000877"
     CDS             11682..12413
                     /gene="fucR"
                     /locus_tag="OO568_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6640339.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGAPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGYLDEVTHIISDERQVATSLVTA"
     gene            complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="OO568_000878"
     CDS             complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="OO568_000878"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6640340.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13550..13945)
                     /locus_tag="OO568_000879"
     CDS             complement(13550..13945)
                     /locus_tag="OO568_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6640341.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13964..14881)
                     /gene="gcvA"
                     /locus_tag="OO568_000880"
     CDS             complement(13964..14881)
                     /gene="gcvA"
                     /locus_tag="OO568_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6640342.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKIPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15240..15467)
                     /locus_tag="OO568_000881"
     CDS             complement(15240..15467)
                     /locus_tag="OO568_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6640343.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15659..16864
                     /gene="csdA"
                     /locus_tag="OO568_000882"
     CDS             15659..16864
                     /gene="csdA"
                     /locus_tag="OO568_000882"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6640344.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16864..17307
                     /gene="csdE"
                     /locus_tag="OO568_000883"
     CDS             16864..17307
                     /gene="csdE"
                     /locus_tag="OO568_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6640345.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17358..18164)
                     /gene="tcdA"
                     /locus_tag="OO568_000884"
     CDS             complement(17358..18164)
                     /gene="tcdA"
                     /locus_tag="OO568_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6640346.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18403..19500)
                     /gene="mltA"
                     /locus_tag="OO568_000885"
     CDS             complement(18403..19500)
                     /gene="mltA"
                     /locus_tag="OO568_000885"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6640347.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGKTHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19708..19784
                     /locus_tag="OO568_000886"
     tRNA            19708..19784
                     /locus_tag="OO568_000886"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19742..19744,aa:Met,seq:cat)
     gene            19818..19894
                     /locus_tag="OO568_000887"
     tRNA            19818..19894
                     /locus_tag="OO568_000887"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19852..19854,aa:Met,seq:cat)
     gene            complement(19969..21222)
                     /gene="amiC"
                     /locus_tag="OO568_000888"
     CDS             complement(19969..21222)
                     /gene="amiC"
                     /locus_tag="OO568_000888"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6640348.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21454..22785
                     /gene="argA"
                     /locus_tag="OO568_000889"
     CDS             21454..22785
                     /gene="argA"
                     /locus_tag="OO568_000889"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6640349.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22847..24673)
                     /gene="recD"
                     /locus_tag="OO568_000890"
     CDS             complement(22847..24673)
                     /gene="recD"
                     /locus_tag="OO568_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6640350.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMLL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24673..28215)
                     /gene="recB"
                     /locus_tag="OO568_000891"
     CDS             complement(24673..28215)
                     /gene="recB"
                     /locus_tag="OO568_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6640351.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEVIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNDDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28208..31096)
                     /gene="ptrA"
                     /locus_tag="OO568_000892"
     CDS             complement(28208..31096)
                     /gene="ptrA"
                     /locus_tag="OO568_000892"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6640352.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKTEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31272..34640)
                     /gene="recC"
                     /locus_tag="OO568_000893"
     CDS             complement(31272..34640)
                     /gene="recC"
                     /locus_tag="OO568_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6640353.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34653..34976)
                     /locus_tag="OO568_000894"
     CDS             complement(34653..34976)
                     /locus_tag="OO568_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6640354.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(34961..35368)
                     /locus_tag="OO568_000895"
     CDS             complement(34961..35368)
                     /locus_tag="OO568_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6640355.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35365..35928)
                     /locus_tag="OO568_000896"
     CDS             complement(35365..35928)
                     /locus_tag="OO568_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6640356.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35919..36389)
                     /locus_tag="OO568_000897"
                     /pseudo
     CDS             complement(35919..36389)
                     /locus_tag="OO568_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36573..37367)
                     /gene="thyA"
                     /locus_tag="OO568_000898"
     CDS             complement(36573..37367)
                     /gene="thyA"
                     /locus_tag="OO568_000898"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6640357.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(37374..38249)
                     /gene="lgt"
                     /locus_tag="OO568_000899"
     CDS             complement(37374..38249)
                     /gene="lgt"
                     /locus_tag="OO568_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6640358.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38400..40646)
                     /gene="ptsP"
                     /locus_tag="OO568_000900"
     CDS             complement(38400..40646)
                     /gene="ptsP"
                     /locus_tag="OO568_000900"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6640359.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSI
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKEMAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40659..41189)
                     /gene="rppH"
                     /locus_tag="OO568_000901"
     CDS             complement(40659..41189)
                     /gene="rppH"
                     /locus_tag="OO568_000901"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6640360.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41874..42563
                     /gene="mutH"
                     /locus_tag="OO568_000902"
     CDS             41874..42563
                     /gene="mutH"
                     /locus_tag="OO568_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6640361.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42632..43345
                     /locus_tag="OO568_000903"
     CDS             42632..43345
                     /locus_tag="OO568_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6640362.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43483..43701
                     /gene="ygdR"
                     /locus_tag="OO568_000904"
     CDS             43483..43701
                     /gene="ygdR"
                     /locus_tag="OO568_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6640363.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43809..44849
                     /locus_tag="OO568_000905"
     CDS             43809..44849
                     /locus_tag="OO568_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6640364.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44882..46075)
                     /gene="lplT"
                     /locus_tag="OO568_000906"
     CDS             complement(44882..46075)
                     /gene="lplT"
                     /locus_tag="OO568_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6640365.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FCPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACVLAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(46068..48227)
                     /gene="aas"
                     /locus_tag="OO568_000907"
     CDS             complement(46068..48227)
                     /gene="aas"
                     /locus_tag="OO568_000907"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6640366.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSVITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48616..49647
                     /gene="galR"
                     /locus_tag="OO568_000908"
     CDS             48616..49647
                     /gene="galR"
                     /locus_tag="OO568_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6640367.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDENGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49654..50916)
                     /gene="lysA"
                     /locus_tag="OO568_000909"
     CDS             complement(49654..50916)
                     /gene="lysA"
                     /locus_tag="OO568_000909"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6640368.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51038..51973
                     /gene="lysR"
                     /locus_tag="OO568_000910"
     CDS             51038..51973
                     /gene="lysR"
                     /locus_tag="OO568_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6640369.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(51960..52652)
                     /gene="ygeA"
                     /locus_tag="OO568_000911"
     CDS             complement(51960..52652)
                     /gene="ygeA"
                     /locus_tag="OO568_000911"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR6640370.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGKFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52781..54194)
                     /gene="araE"
                     /locus_tag="OO568_000912"
                     /pseudo
     CDS             complement(52781..54194)
                     /gene="araE"
                     /locus_tag="OO568_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(54509..55270)
                     /gene="kduD"
                     /locus_tag="OO568_000913"
     CDS             complement(54509..55270)
                     /gene="kduD"
                     /locus_tag="OO568_000913"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6640371.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55300..56136)
                     /gene="kduI"
                     /locus_tag="OO568_000914"
     CDS             complement(55300..56136)
                     /gene="kduI"
                     /locus_tag="OO568_000914"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6640372.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(56456..57604)
                     /locus_tag="OO568_000915"
     CDS             complement(56456..57604)
                     /locus_tag="OO568_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6640373.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKAQALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGG"
     gene            57859..58365
                     /locus_tag="OO568_000916"
                     /pseudo
     CDS             57859..58365
                     /locus_tag="OO568_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
BASE COUNT        14346 a        15739 c        14288 g        13992 t
ORIGIN      
        1 cggtgacgtt tgttgccggg ttttctcgtt tttggtcact tactcatcaa ctcatttcat
       61 ttgttatatg aatgtttctt accaccctca cgcgacaaat atcatcacag ttaatatgcc
      121 atacaattta tgttgcaacg caaacgtttc cctattttca taaaccgtat tttatcgcta
      181 tgaaaaagaa attatcgcca tgattaacta aaagtattga ttttttcagt tcaacctaca
      241 tatattgcgc gccccggaag aagtcagatg tcgtttaatg ggcaaatatt gcccttaaat
      301 tctcttttac ttttgattta cagagtaaag cgttgggata atctatcttc caagcagatt
      361 attgtatttg ggatcaagat cactgataga tacataactt gtgtgtatct ttccgccctc
      421 aaattattac agcggtaaat gattaagcca tcgccgatag acagatttca tttttacggt
      481 caggcacctt cccgggctga actggctaaa agctgaatta tttgcattcc tccaggagaa
      541 atagatggaa acgactcaaa ccagcacgat tgcgtcgaaa gactctcgta gtgcctggcg
      601 caagacagac accatgtgga tgctgggcct ttacggcacg gcaatcggcg cgggcgtgct
      661 gttcctgcca atcaacgccg gtgttggcgg tatgatcccg ctgatcatca tggctatcct
      721 tgcgttcccg atgacgtttt ttgctcaccg cggcctgact cgcttcgtac tgtctggtaa
      781 aaacccgggc gaagacatca ccgaggttgt agaagaacac tttggtattg gcgcaggtaa
      841 actgattacc ctgctctact tcttcgctat ctacccgatc ctgctggttt atagcgtggc
      901 aatcaccaat accgttgaaa gcttcatgtc tcaccagctg agtatgacgc caccgccgcg
      961 tgcgattctg tcgctgatcc tgatcgtggg tatgatgacc atcgttcgct ttggtgagca
     1021 gatgatcgtt aaagcgatga gtattctggt attcccgttt gttggcgtac tgatgctgct
     1081 ggctctgtac ctgatcccgc agtggaacgg cgcagcactg gaaacgctgt ctctggacac
     1141 tgcatctgca accggaaacg gtctgtggat gaccctgtgg ctggcaattc cggtaatggt
     1201 gttctcgttc aaccactctc cgatcatctc ttctttcgcc gttgcgaagc gtgaagagta
     1261 cggcgatatg gcagaacaga aatgctcgaa gatcctggca ttcgcacaca tcatgatggt
     1321 gctgaccgta atgttcttcg tcttcagctg cgtactgagc ctgactccgg cagacctggc
     1381 tgcggctaaa gagcagaaca tctcgattct gtcttacctg gctaaccact ttaacgcacc
     1441 ggttatcgcg tggatggctc cgattatcgc gattatcgct atcaccaaat ccttcctcgg
     1501 ccactacctg ggcgcacgtg aaggcttcaa cggtatggtg attaaatctc tgcgtggtaa
     1561 aggtaagtct atcgaaatca acaagctgaa ccgtatcact gcgctgttca tgctggtaac
     1621 gacctggatt gttgccaccc tgaacccgag catcctgggt atgattgaaa ccctgggcgg
     1681 cccaatcatc gcgatgatcc tgttcctgat gccgatgtac gcaattcaga aagtaccggc
     1741 aatgcgtaag tacagcggtc acatcagcaa cgtattcgtt gtcgtgatgg ggctgattgc
     1801 aatctccgca atcttctact ctctgttcag ctaagtcctt tcgcgccgct ttcgggcggc
     1861 gcttcctccg ttttaacgcg atgtatttcc tatgattagc gtattcgata ttttcaaaat
     1921 cggcattggc ccttccagtt ctcataccgt tggaccaatg aaagcgggta aacaatttac
     1981 cgacgatctg attgcccgta acctgcttaa agacgtgacc cgcgtggtgg ttgacgtgta
     2041 cggctcgctc tctctgactg gtaaaggcca ccacacagat atctccatta ttatgggcct
     2101 ggcgggtaac ctgccggata ccgtggatat cgattccatc cccggtttta ttcaggatgt
     2161 gaatactcat ggtcgcctga tgctggcaaa cggtcagcat gaagtggagt tcccggttga
     2221 tcagtgcatg aacttccatg cagacaacct ttctctgcat gaaaacggta tgcgcattac
     2281 cgcgctggcg ggcgataaag tcgtttacag ccagacttac tactctattg gcggtggctt
     2341 tatcgttgat gaagagcatt ttggccagca ggatagcgca ccggttgaag ttccttatcc
     2401 gtacagttca gcagccgatc tgcaaaaaca ttgtcaggaa accgggctgt cactctctgg
     2461 cctgatgatg aaaaacgagc tggcgctgca cagtaaagaa gagctggaac agcacctggc
     2521 gaacgtctgg gaagtcatgc gtggcggtat tgagcgcggt atttccaccg aaggcgtgtt
     2581 gcctggcaaa ctgcgcgttc cacgccgtgc tgcggcacta cgccggatgc tggtcagcca
     2641 ggataaaacc accactgacc cgatggcggt tgttgactgg atcaacatgt ttgcactggc
     2701 agtgaacgaa gagaacgctg ctggcggtcg cgtggtgact gcgccgacta acggtgcgtg
     2761 cgggattatc ccggctgtgc tggcgtacta cgataagttt atccgcgaag tgaacgctaa
     2821 ctcactggct cgttacctgc tggtagccag cgccattggt tctctttata agatgaacgc
     2881 gtcgatttct ggtgccgaag tgggttgcca gggtgaagtt ggcgtggcgt gctctatggc
     2941 ggcagctggt ctggcagaac tgttaggcgc aagcccggcg caggtttgca tcgcagcgga
     3001 aatcgcgatg gagcacaacc tcggtctgac ttgtgacccg gtcgcggggc aggttcaggt
     3061 accgtgcatt gagcgtaacg ccattgcagc agtgaaagcg gtgaacgccg cgcgtatggc
     3121 actgcgccgt accagcgaac cgcgcgtctg cctcgataaa gttatcgaaa ccatgtacga
     3181 aacaggtaaa gacatgaacg ccaagtaccg cgaaacctct cgcggcggcc tggcgatgaa
     3241 gatcgttgcc tgcgattaat ctctcccaga ggcctcgttt tgcgaagcct cttcctgttt
     3301 tctcaaccag ccatagcctg ttccggcatc gaatgttacc cttatcgcct gatctttaag
     3361 ggggttatcg tggctgttca tttgcttatt gtcgatgcac tgaatcttat ccgtcgcatt
     3421 catgccgttc agggttcgcc ctgtgtcgaa acctgccagc atgcgctcga tcagctcatt
     3481 atgcacagcc agccaaccca cgcggtcgcc gtttttgatg atgaaaaccg cagtagcggc
     3541 tggcgtcatc agcgtttacc agattacaaa gcggatcgac cgccaatgcc ggaagagttg
     3601 cacgacgaga tgcctgcatt acgcgccgcc tttgagcaac gcggcgtccc gtgctggtca
     3661 gccagcggca acgaagccga tgacttagcc gccacactgg cggtcaaagt gacactggcc
     3721 gggcatcagg caacgattgt ttcgacagat aaaggctact gtcagttact ttcaccgaca
     3781 ttacgtattc gtgattactt ccagaaacgt tggctggatg cgccatttat cgataaagaa
     3841 tttggcgttc aaccgcagca gttgcccgat tactggggac ttgcggggat cagcagttca
     3901 aaggtaccgg gtgttgcggg aatcggacca aaaagcgcca cgcagctgct ggtcgagttt
     3961 cagagtctgg aagggatata tgagaatctg gatgcggttg ccgaaaagtg gcgcaaaaaa
     4021 ttagaaaccc ataaagagat ggcgtttctg tgccgcgata ttgcccgctt acaaaccgat
     4081 ttgcatatcg acggcaattt acagcaattg cggttggtac ggtaacggcg agccggatac
     4141 gccgcaaatc gtatccggca ttatcacatc agcgcattta ccaggcggta tggtaaagct
     4201 ctacaatatc ctcaagcgtt gcttcacgcg ggttgccacc ggtacaaaca tcatccagtg
     4261 ccgcctgagc cagtgccgaa atgtcttcct tgcgtacacc aacatcacgc aaatgtggcg
     4321 gaataccgac atcacggttg agggcaaaca ccgcttcaac agcggcatta cgcgcccctt
     4381 ccaggctcat accttccact ttcacgccca taacgcgcgc gatatcgcgg tacttctcac
     4441 cggtaaagtc agcgttatag cgcatgacat gcggtaacag gatggcgttc gcaacaccgt
     4501 gtggagtgtt ataaaacgcg cccagtggat gcgccatacc atgcaccaac cctaacccaa
     4561 cattcgagaa gcccataccc gcaacatact gcccgagcgc catttcttct ccggcatcct
     4621 tatcaccagc aaccgatcct cgcagcgccc cagcaatgat ttcaatcgct ttaatgtgca
     4681 gtgcatcggt tagtgcccac gcgccacggg taatataccc ctcaatagca tgagtgagcg
     4741 catcgacacc cgtcgcggct ttcagcgccg gaggcatacc atccatcatg tcagcatcaa
     4801 taaacgccac ctgcgggata tcatgcggat caacgcaaac aaacttgcgc cgtttttctt
     4861 cgtcagtgat cacgtagtta atggtcactt ctgccgcagt acctgctgtg gtggggattg
     4921 ccagaatcgg tacactgggt ttattggtcg gggaaagccc ttccaggcta cgcacatcgg
     4981 caaactccgg gttgttgctg ataatgccaa ttgctttaca agtatcctgt ggagaaccac
     5041 caccaatagc gatcaggtaa tccgcgccgc tattctggaa tacaccgagc ccttctttga
     5101 cgacagtaat tgttgggttg ggcactacgc cgtcgtaaat cgcccatgcc agccctgcag
     5161 catccatctt atcggtcact ttcgccacca cgccgcattg caccagcgtt ttatcggtga
     5221 cgatcagcgc cttctgataa ccacggcgtt tcacctcatc ggttaaagcc ccaacagcac
     5281 cccgaccaaa ccatgccgtt tcgttcagaa tcattctgtt agccatcatc cttctccttg
     5341 ttgctttacg aaattactct tcaatccgta acccataggt tttgaatttc tccagcacta
     5401 cggcaatctc ttcatcgctc agcactggca ccgggtccgt aatcgccagg gtcgtcaggt
     5461 aaagttgcgc cagcacttca acttcatgcg ccagccataa cgctttttcc agattcacct
     5521 cacaagcgat aagcccatga tgttgtaaca aagttgcctt acgatttttg agagccagcg
     5581 caacatgttc agaaagttcg cgtgttccaa aggtcgcata aggcgcgcaa ggaatagaat
     5641 taccgccagc cgccgcaatc atgtagtgaa tagcggggat cgatcggtta agaatggaaa
     5701 ctgccgtgca atgaacggca tgattgtgaa caaccgcgtt ggcatccggt ctgctttgat
     5761 aggctgccat atggaaacgc cattcgcttg aggggagctt tccttcctca tgtttaccgt
     5821 tgccatcaat aaagacaata tgcgactccg tcagtttttc atatggaatg cctgtaggcg
     5881 taatcagcat cccatcctga taacgtacac tgacgttccc cgctgtcccc tggttcagtc
     5941 ccaggcgggt catttccagg caagtgtcaa taatctgacg agcaagttta tttcgttcca
     6001 ttagctacct ctctctgatt caaaacaggg caataatgtt gttcctttca cactattgaa
     6061 ttagccgttt aattacccac catcttcttc ctgattaaca agaaagaaat tcacaagctt
     6121 atattttgtg acctagctca actaatcaca gtaaataact gcaagttctc tttttataac
     6181 cccgttaaaa atgaccgctc ttaaaaatat ttatcaaaac ggtcattttt ccatttcccc
     6241 tcgccagaaa tgcccgtttt cggcacaaac aattaatacg gtcatctggt ttgtgttttt
     6301 tatgatttat tttctgaaac gggcatgaaa tttcgattat taaagtgatg gtagtcacat
     6361 aaagtcacct tccagctaat aagtgtgacc gccgtcatat tacagagcgt tttttatttg
     6421 aaaatgaatc catgagttca tttcagacag gcaaatattc accgatatga agcccgaact
     6481 cgctggtttt gcacttttga aaacataacc gattacgtgc ttaagcttct gaacctaaga
     6541 ggatgctatg ggaaacacat caatacaaac gcagagttac cgtgcggtag ataaagatgc
     6601 agggcaaagc agaacttaca ttattccatt cgcgctgctg tgctcactgt tttttctttg
     6661 ggcggtagcc aataacctta acgacatttt attacctcaa ttccagcagg cttttacgct
     6721 gacaaatttc caggctggcc tgatccaatc ggccttttac tttggttatt tcattatccc
     6781 aatccctgct gggatattga tgaaaaaact cagttataaa gcagggatta ttaccggatt
     6841 atttttatat gccttgggcg ctgcattatt ctggcccgct gcagaaataa tgaactacac
     6901 cttattttta gttggcctat ttattattgc agccggatta ggttgtctgg aaactgccgc
     6961 aaaccctttt gttacggtat tagggccgga aagcagtagt cacttccgct taaatcttgc
     7021 gcaaacattt aactcgtttg gcgcaattat cgcggttgtc tttgggcaaa gtcttatttt
     7081 gtctaacgtg ccacatcaat cgcaagacgt tctcgataaa atgtctccag agcaattgag
     7141 tgcgtataaa cacagcttgg tattatcggt acagacacct tatatgatca tcgtggctat
     7201 cgtgttactg gtcgccctgc tgatcatgct gacgaaattc ccggcattgc agagtgataa
     7261 tcacagtgac gccaaacaag gatcgttctc cgcatcgctt tctcgtctgg cgcgtattcg
     7321 tcactggcgc tgggcggtat tagcgcaatt ctgctatgtc ggcgcacaaa cggcctgctg
     7381 gagctatttg attcgctacg ctgtagaaga aattccaggt atgactgcag gctttgccgc
     7441 taactattta accggaacca tggtgtgctt ctttattggt cgtatcaccg gtacctggct
     7501 catcagtcgc ttcgcaccac acaaagtcct ggccgcctac gcattaatcg ctatggcact
     7561 gtgcctgatc tcagccttcg ctggcggtca tgtgggctta atagccctga ctttatgcag
     7621 cgcctttatg tcgattcagt acccaacaat cttctcgctg ggcattaaga atctcggcca
     7681 ggacaccaaa tacggttcgt ccttcatcgt tatgaccatt attggcggcg gtattgtcac
     7741 tccggtcatg ggttttgtca gtgacgcggc gggcaacatc cccactgctg aactgatccc
     7801 cgcactctgc ttcgcggtca tctttatctt tgcccgtttc cgttctcaaa cggcaactaa
     7861 ctgaacatat tttccgaata aagtgaggaa tctgtaatga aaaaaatcag cttaccgaaa
     7921 attggtatcc gcccggttat tgacggtcgt cgcatgggtg ttcgtgagtc gcttgaagaa
     7981 caaacaatga atatggcgaa agctacggcc gcactgctga ccgagaaact gcgccatacc
     8041 tgcggagctg ccgtcgagtg tgtcatttcc gatacctgta tcgcgggtat ggctgaagcc
     8101 gctgcttgcg aagaaaaatt cagcagccag aatgtaggcc tcaccattac ggtaacgcct
     8161 tgctggtgct atggcagtga aaccatcgac atggatccaa cccgcccgaa agccatttgg
     8221 ggctttaacg gcactgaacg ccccggcgct gtttacctgg cagcggctct ggcagctcac
     8281 agccagaaag gcatcccggc attctccatt tacggtcatg acgttcagga tgccgatgac
     8341 acatcgattc ctgccgatgt tgaagaaaaa ctgctgcgct ttgcccgcgc cggtttggcc
     8401 gtcgccagca tgaaaggtaa aagctatctg tcgctgggcg gcgtttcgat gggtatcgcc
     8461 ggttctattg ttgatcacaa cttctttgaa tcctggctgg gaatgaaagt ccaggcggtg
     8521 gatatgaccg aactgcgtcg ccgtatcgat cagaagattt acgacgaagc cgaactggaa
     8581 atggcactgg cctgggctga taaaaacttc cgctatggcg aagatgaaaa taacaaacag
     8641 tatcaacgca atgccgagca aagccgcgca gttctgcgcg aaagtttact gatggcgatg
     8701 tgtatccgcg acatgatgca aggcaacagc aaactggccg atattggtcg cgtggaagaa
     8761 tcacttggct acaacgccat cgctgcgggc ttccaggggc aacgtcactg gaccgatcaa
     8821 tatcccaatg gtgacaccgc cgaagcgatc ctcaacagtt catttgactg gaatggcgtg
     8881 cgcgaaccct ttgtcgtggc gaccgaaaac gacagtctta acggcgtggc aatgctaatg
     8941 ggtcaccagc tcaccggcac cgctcaggta tttgccgatg tgcgtaccta ctggtcacca
     9001 gaagcaattg agcgtgtaac ggggcataaa ctggacggac tggcagaaca cggcatcatc
     9061 catttgatca actcaggttc tgctgcgctg gacggttcct gtaaacaacg cgacagcgaa
     9121 ggtaacccga cgatgaagcc gcactgggaa atctctcagc aagaggctga cgcttgcctc
     9181 gccgctaccg aatggtgccc ggcgatccac gaatacttcc gtggcggcgg ttactcttcc
     9241 cgcttcctta ccgaaggcgg cgtcccgttc accatgactc gtgtcaacat catcaaaggc
     9301 ctgggaccgg tactgcaaat cgcggaaggc tggagcgtgg aattgccgaa ggatgtgcat
     9361 gacatcctca acaaacgcac caactcaacc tggccaacca cctggtttgc accgcgcctc
     9421 actggtaaag ggccgtttac ggatgtgtat tcggtaatgg cgaactgggg tgccaaccac
     9481 ggagttctga ccatcggcca cgttggcgca gactttatca cccttgcctc catgctgcgt
     9541 atcccggtat gtatgcacaa cgttgaagag accaaagtgt atcgtccttc tgcctaggct
     9601 gcgcacggca tggatattga aggccaggat taccgcgctt gccagaacta cggtccgttg
     9661 tacaagcgtt aatacattac accctgatgt gatgcccggt cgctccggct accgggcctg
     9721 aacaagcaag agtggttagc cggataaaca atgttatccg gctatattgc aggagcgatt
     9781 atgaaacaag aagttatcct ggtactcgac tgtggcgcga ccaatgtcag ggccatcgcg
     9841 gttaatcggc agggaaaaat tgttgcccgc gcctcaacgc ctaatgccag cgatatcgcg
     9901 atggaaaaca acacctggca ccagtggtct ttagactgcc attttgcaac gctttgctga
     9961 ttgctgtcgg caaatcaata gtgaactgac tgaatgccac atccgcggta tcgccgtcac
    10021 cacctttggt gtggatggcg ctctggtaga taagcaaggc aatctgctct atccgatcat
    10081 tagctggaaa tgtccgcgaa cagcagcggt tatggacaat attgaacggt taatctccgc
    10141 acagcggttg caggctattt ctggcgtcgg agcctttagt ttcaatacgt tatataagtt
    10201 ggtgtggttg aaagaaaatc atccacaact gctggaacgc gcgcacgcct ggctcttcat
    10261 ttcgtcgctg attaaccacc gtttaaccgg cgaattcact actgatatca cgatagccgg
    10321 aaccagccag atgctggata tccagcaacg cgatttcagt ccgcaaattt tacaagccac
    10381 cggtattcca cgccgactct tccctcgtct ggtggaagcg ggtgaacaga ttggtacgct
    10441 acagaacagc gccgcagcaa tgctcggctt acccgttggc ataccggtga tttccgcagg
    10501 tcacgatacc cagttcgccc tttttggcgc tggtgccgaa caaaatgaac ccgtgctctc
    10561 ctccggtaca tgggaaattt taatggttcg cagcgcctag gttgatactt cgctgttaag
    10621 tcagtacgcc ggttccacct gcgaactaga tagccaggca tggttgtata acccaggtat
    10681 gcaatggctg gcatccggcg tgctggaatg ggtgagaaaa ctgttctgga cggctgaaac
    10741 accctggcaa atgttgattg aagaagctcg tctgatcgca cctggcgcgg atggtgtgaa
    10801 aatgcagtgt gatttattgt cgtgtcagaa cgctggctgg caaggagtga cgcttaatac
    10861 cacgcggggg catttctatc gcgcggcgct ggaagggtta actgcgcaat tacagcgcaa
    10921 tctacagatg ctggaaaaaa tcgggcactt taaagcctat gaattattgt tagtcggtgg
    10981 aggaagtcgc aacacattgt ggaatcagat taaagccaat atgcttgata ttccggtaaa
    11041 agttctcgac gatgccgaaa cgaccgtcgc aggagctgcg ctgttcggtt ggtatggcgt
    11101 aggggaattt aacagcccgg aagaagcccg tgcacagatt cattatcagt accgttattt
    11161 ctacccgcaa actgaacctg aatttataga ggaagtgtga aatgctgaaa acaatttcgc
    11221 cgttaatttc tcccgaacta ttgaaagtgc tggcagagat gggacatgga gatgaaatta
    11281 ttttttccga tgctcacttt cccgcccatt cgatgggacc gcaggtgatc cgcgctgatg
    11341 gcctgttggt gagcgacttg ctccaggcga ttatcccgtt atttgagctg gacagttatg
    11401 ccccgccgct ggtgatgatg gcggcggtag agggtgacac tctcgatcct gaagtagaac
    11461 gacgttaccg taatgcgctt tcactacaag ccccgtgtcc tgacatcatc cgcatcaatc
    11521 gttttgcgtt ttatgaacgg gcgcaaaaag cctttgcgat cgttatcaca ggcgaacgag
    11581 cgaagtacgg gaatattctt ttaaaaaaag gggtaacacc gtaatctcat accggtacgc
    11641 ccgcatgacg cgggcggtta tcgaatgatg gggtgaaaaa tatgaaagcg gcacgccagc
    11701 aagcgatagt cgacctgctg ctgaaccata ccagtctgac cacggaagct ctctctgaac
    11761 agctaaaggt cagtaaagaa accattcgtc gcgatctcaa tgaattacag acgcagggta
    11821 aaattctgcg caatcatgga cgcgctaaat atatccaccg tcaaaatcaa gacagtggcg
    11881 ctccctttca catcaggctg aaaagccatt atgcgcataa agcagatatc gcgcgcgagg
    11941 cgctcgcgtg gattgaagaa gggatggtga tagccttaga cgccagttca acttgctggt
    12001 atctggcacg ccagttgcct gacatcaaca ttcaggtctt caccaatagc catccgattt
    12061 gccatgaact cggtaaacgc gaacgcattc aactgatcag ttccggcggc acacttgagc
    12121 gcaaatatgg ctgttacgtc aatccctcgc tgatttccca acttaaatcg ctggaaatcg
    12181 atctgtttat tttttcttgt gaagggatcg atagcagcgg cgcactgtgg gactccaatg
    12241 cgatcaacgc tgattacaaa tcgatgctat taaaacgtgc cgcgcaatcg ttgttattga
    12301 ttgataaaag taaatttaat cgttcagggg aagcccgcat cgggtatctg gatgaggtaa
    12361 cgcacattat ttctgatgag cgccaggttg caacttcttt ggtaacagcc tgacggaaag
    12421 ggtagcaggc cggagacgac gccccggcct tgcctgttat cgctcgtcgc gacgaccacc
    12481 caccgcagcc cagatgcggc ggacgtgcac cgtcacttct tcgcgatcgt gatacaactg
    12541 ccgcgcctga atctgagcat ttatgccatg ttcatcaagc tgtgcctgaa tatacgccag
    12601 attgtgtgat acttcttcgt agcgtttttt catcggcagt ttgagattga aaatggtttc
    12661 acggcaccag ccattaacca gccactgcgc catcaatgcc gcaactttag ccggtttttc
    12721 aaccatatcg cataccatcc aggagatatt gctgcgtgtc ggacggaatt tgaaaccgtc
    12781 ttcccgcagc cacgtcacct gcccggtatc catcagactt tgcgccatcg ggccgttgtc
    12841 gacggaataa acccacatgt tgcgcttcac cagttggtag gtccagccgc cagggcaagc
    12901 gcctaaatcc accgcccaca tcccgttcgc caggcgttca tcccactcat ccgcaggaat
    12961 aaacacatga aatgcctctt ccagtttgag cgtggaacga ctcggcgcct ctgccggaaa
    13021 tttcaggcgc ggaatgccca tatagaacgg cgaattattg ttgctgtatg agtaaccggt
    13081 atagcagcat cctggtgcaa taaagaatac atgcacaacc ggacgcttcg gcgtttcata
    13141 gttcgccagc accccagcat cgcgcaaggc agcgcgtagc ggaacagtga acttacggca
    13201 gaatttcagc aactctttgc tttcgttggt atcggcaact tcaacacgca gttcaccgcc
    13261 cttctctact acaccctgta acatgccgac aatgggggta atacgatctt ctggcggcaa
    13321 atgctgcagg agttccccca ccacaaacca ctggcgggca aaaattaatg aactgaacgg
    13381 cagctcacgg attaacttat cgccatcatc aggttgataa cattcataaa tgacataacc
    13441 cgcattctct ttcacgcggg caaaaccgaa aatttcccgc tggccggctt tatcggtaat
    13501 ttctgcggcg cactcttttt caaagcccgg acggcacagc aatacaacct tattcatgac
    13561 ttacgccctt gcgctttaaa cggatagcac caactaacat taacgcccag cccgcaagga
    13621 agctcacgcc gccaaccggt gtgacaaacg cccacaaacg cagatgggac agcgccaggc
    13681 aataaaggct gccgctgaac agcaccgtgc ctaacgcgag gaaaacgcta ctccagtaaa
    13741 accagatact gatgcgacgc tgcattgcca ccgccagacc taagatcgcc agcgtatgaa
    13801 acgcctggta ttcgagaccg gtttggatcc agcccatctc aacggccccc atggttttac
    13861 ttaacacatg cgcaccaaaa gcgcccagtg ccacaaaaat gaagccgcta atggcggcga
    13921 aaatcagcat aaaacggctg gtcatggtcg taccctacgt aaattattgt tcataacgaa
    13981 agcggaattt ttcttgttca gcagcggctt tcgccaggat ccattggcga aaggcggcta
    14041 ttttacccag ttctgcctgg ctgtcatgac aaaccagata aaaagcattt ttactgacca
    14101 gaacatcatt aaacgggcaa acaagacgtc cggcctcgat ttcagattgc gccatcacgt
    14161 tatttgccag cgccactccc tgcccgtgaa tagccgcttg cagcaccatg gcgctatgac
    14221 taaaaattgg cccttgctga acgttgatat gatttaaccc caactgtcgg gtatatgtct
    14281 gccagtcacg gcgtgacgca tcatgtaata acgtatgttt agccagatct tccgggatct
    14341 tcaagggttt ttcgcctgtc agcagtagcg gcgaacacac cggcaataaa tattcggcgt
    14401 acagtttttc cacccgtagc cccggccagt tgccccgacc ataaaatatc gccacatcaa
    14461 catcatccgc cagcttatct tcctgacgat caaccgcctg gattcgaacg tcaattcccg
    14521 gataagctga attaaagctg gaaagtcgcg gaaccaacca atgaatggcg aaactgggga
    14581 gtaaactgac cgtcaacgcc cccttggcgc tacgggcctg gagtttacgc gtcgcttcgg
    14641 ttaattgcga aaatatctct ttgatatcga ggaaatagct ttgcccttcc tcggtcagca
    14701 ggagtgaacg attacggcgg cggaacagtt ttagccccaa aaaatcctca agagacttga
    14761 tttgatgact tactgcggcc tgggtcacaa aaagctcttc tgctgcgcga gtaaaactta
    14821 aatggcgtgc tgcggcatca aaaactcgta aggcatttag cggtggtaat cgtttagaca
    14881 tggcttttaa actttgatgt taaatgaatt taacaattag atcacactgt gtaacctatt
    14941 agttttttta atctgagcca ttataaattg tccgttgagc ttataccagc aaatacctat
    15001 agtggcggca cttcctgagc cggaacgaaa agttttatcg gaatgcgtat tctggtgaac
    15061 ttttggctta cggttgtgat gttgtgttgt tgtgttgttg tgtttgcaat tggtctgcga
    15121 ttcagaccat ggtagcaaag ctaccttttt tcacttcctg tacatttacc ctgtctgtcc
    15181 atagtgatta atgtagcacc gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat
    15241 tactgttcca gttcgaccat ctctttcacg tcagtacggt tgatctgctg tttgttgcca
    15301 ttagcgtctt tatacgaaat cataccggta tcgttatcag tctgtggttt gccgtcagag
    15361 acgatggtac gtccgtcatt ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg
    15421 gcaaaagtca gcatacaggc agaaataatt gcggcagtct ttttcatcat cttctcctta
    15481 aagcgattaa taaatcttaa tagtattgat taacaggcta aaattaacgc ctaacactat
    15541 tcagcatatg ttacttacgc ggcttcgcca ggatatccag ataattctga tggttagcac
    15601 tctccttgta tcaaagtgaa ttttgcgtca cgatcggtgc atcaagccga ggagtaccat
    15661 gaacgttttt aatcccgcgc agtttcgcgc ccagtttccc gcactacagg atgcgggcgt
    15721 ctatctcgac agcgccgcga ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca
    15781 gttttacagc ctgagcgccg gaaacgtcca tcgcagccag tttgccgaag cccaacgcct
    15841 gaccgcgcgt tatgaagctg cacgagagaa agtggcgcaa ttactgaatg caccggatga
    15901 taaaactatc gtctggacgc gcggcaccac tgaatccatc aacatggtgg cacaatgcta
    15961 tgcgcgtccg cgtctgcaac cgggcgatga gattattgtc agcgtggcag aacaccacgc
    16021 taacctcgtc ccctggctga tggtcgccca acaaactaga gccaaagtgg tgaaattgcc
    16081 gcttaatgcg cagcgactgc cggatgtcga tttgttgcca gaactgatta ctccccgtag
    16141 tcggattctg gcgttgggtc agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg
    16201 agcgattacc tttgctcatt cagccgggat ggtggtgatg gttgatggtg ctcagggggc
    16261 ggtgcatttc cccgcggatg ttcagcaact ggatatcgat ttctatgctt tctctggtca
    16321 caaactgtat ggcccgacgg gtatcggcgt gctgtatggc aaatcagaac tgctggaagc
    16381 gatgtcgccc tggctgggtg gcggcaaaat gattcacgaa gtgagttttg acggtttcac
    16441 cactcaatct gcgccgtgga aactggaagc aggaacgcca aatgtcgctg gcgtcatagg
    16501 attaagcgcg gcgctggaat ggctggcaga ttacgatatc aaccaggccg aaagctggag
    16561 ccgtagctta gcaacgcttg cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt
    16621 ccgctgccag gattccagcc tgctggcctt tgattttgct ggcgttcatc acagcgatat
    16681 ggtgacgcta ctggcggagt acggtattgc cttgcgggcc gggcaacatt gcgctcagcc
    16741 gctactggca gaattaggcg taaccggcac actgcgcgcc tcatttgcgc catataatac
    16801 aaagagtgat gtggatgcgc tggtgaatgc cgttgaccgc gcgctggaat tattggtgga
    16861 ttaatgacaa acccgcaatt cgccggacat ccgttcggca caaccgtaac cgcagaaacg
    16921 ttacgcaata ccttcgcacc gttgtcgcaa tgggaagata aatatcgcca gttgatcatg
    16981 ctggggaaac agcttccggc tttgccagac gagttaaaag cgcaggctaa agagattgcc
    17041 ggatgcgaaa accgcgtctg gctgggatat accgtggctg aaaacggcaa aatgcacttc
    17101 tttggcgaca gcgaagggcg cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt
    17161 gaggggaaaa ccgccgccga attacaggca cagtcgccgc tggcattgtt tgatgagctg
    17221 ggattacgtg cgcagcttag cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg
    17281 attatcgctg cgacgaagca ggtttaagca cagaaccggg tcggataaga cgttcgcgtc
    17341 tcatccgacc tgattgttta accctgacgc gccgctttcg ccatcatctt cttcagcgca
    17401 tgagaaaccg caacaaaccc aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat
    17461 gcacaatcca tccgcttcgg cccttctgcc gtggctttca tcgcacacac cgtaccgtct
    17521 gactgcgggt acaccagcgc ttcagtagaa aacacgcaat ccacgccgag cttaccttta
    17581 ctgtttttca ctacgccaaa atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga
    17641 tcctgaatcg ttttcgccag atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc
    17701 gcgccaccgg tcgtgaccag cgggatttta ttgcgccgac aataagcaat cagcgccgct
    17761 ttgggccgta cactatcaat ggcatcaatc acgtacgaat aaccggcact catatattgc
    17821 gctacgttat ccggcgtcac gaaatcatcc accaccgtta cacggcactc cgggttaatc
    17881 tggcgaatgc gctccgccat gacttccgct tttgccagcc cgacgttatc gcgcagagcg
    17941 tgaatttgcc gattggtatt ggtgacgcac acatcatcca tatcgataag cgtgattgcg
    18001 ccaatccccg tgcgcgccag cgcttccgcc gcccaggaac cgacaccacc gataccaacc
    18061 acacaaatat gcgcgtcagc aaacagttgc aacgcttttt caccatacag acgcgctgtg
    18121 ccaccaaaac gctgacgcca tgcatcacta attaccacag acatagcacc tcagaattaa
    18181 aaagggcgag gttatccctc accctaaacg cataaatgcc tgatgcgcta cgcttatcag
    18241 gcctacataa ctcctgcaat attttggatt tgcatgattt gtaggccgga taaggcgttc
    18301 acgccgcatc cggtatgaac aacgcgcact tagtcaataa tcaggatgtg aggttacccc
    18361 tcaccctgtc atatccgtaa aaacggcata cagaatatca catcagccgc taaagacgtt
    18421 acctgcgccc ggagccgttt tcagcaccca aacccgtcca tagtggttgt accaacctgc
    18481 gcggtgtccg gcttccggcc cgatcccttg atagatatcg aagtgttggc ctttgattgc
    18541 gcccccgaca tccaacgcca ccatcagacg caattcgtac tgaccgttaa atttgccgtt
    18601 attatccagc agcggcactt ctgccagcaa ggtagtacct ggcggaataa ttgaacgatc
    18661 ggaggcaaca gaagcacggc caaccagcgg caccgcactt gcccctttca ccggtgcaaa
    18721 agattgcggt ttaaagaaga cgaaagacgg gttctgttcc agcagctcgc gaacctcggc
    18781 ttcactgtgt gttttgcccc aatgacgaat cgcctgcatc gacatatctt cttttttcac
    18841 ttcgccacgg tcgatcagca ccttaccaat gctgcgatag gcatgaccgt ttttccctgc
    18901 atagctgaaa aagttaagcg gactgccatc accaaagtcg atatacccac taccctgcac
    18961 atccataatg aagttatcca tcagagagtt gctgtaagcg aggatgtatt tatcgcccaa
    19021 tgccccggcg tagatctccg cacgagacgg cagacgacca cgttttggcg gcatacggta
    19081 aataggatac tggaactcgc cctggcgggt atggcgcgcc tgaattaccg gcgtgtaata
    19141 gcctgtaaac tgcacgttac catagttgtc ggcaccttcc atctgccagg catcaatgcc
    19201 gaactggcgc atattgcggg tatcaccgcc tgcgcgcagc cactcttgca ccgcgttata
    19261 aacattactc tggttgccat acagacgcgg tgacgaatta cggatatggt taatttgctc
    19321 ggcaaaatca ccggcgttaa tcggcgcgcc aacggcatct ggctggttca ccagagagaa
    19381 aggctgggta aatttcccgt ctttatattg ctgtccgcga tcggttggtt tggaagagca
    19441 ggcggcaagc attgccacaa ccgtgcccat aagaaggtac tttacccaac gtcctttcat
    19501 tgttctcttc ttcaggttaa aaaataaggc gcaacgaaga taacaaaccg ccgcgtttaa
    19561 tgaaatgagc agcctctccc tgacgcaaat tttgcacaaa aaataagctt agtggtttgt
    19621 ttttgttcaa aatcatgcta aatccgtgat cggggtaaaa aaaaggttgc atgaaaacgc
    19681 gagcggagta tagtgcgcat ccacggacgc ggggtggagc agcctggtag ctcgtcgggc
    19741 tcataacccg aaggtcgtcg gttcaaatcc ggcccccgca accaatcaaa tttgatgaag
    19801 taaaagcagt acggtgacgc ggggtggagc agcctggtag ctcgtcgggc tcataacccg
    19861 aaggtcgtcg gttcaaatcc ggcccccgca accaattatt gaacacccta acgggtgttt
    19921 ttttgtttct gatctcccat aaaaaaagcg ccattcagcg cctttttatc atccccttct
    19981 cgccagcgtt gccccatcgg caaaatacgc tttaatcccc gcaagaatag actccgcaac
    20041 ttcctgctgg aaagtcgccg ttttcagttt acgctcttcc tcaacgttac tgataaacgc
    20101 cgtttcgacc agaatggagg gaatatctgg tgcctttagt acggcaaacc cggcctgttc
    20161 aacttgattt ttatgcagct tgttgatttt tccgagctta ttcagtaccg ctttaccaaa
    20221 cttaaggctg tcagcaatag tcagcgactg caccatatcg aacatggtgt ggtcgacata
    20281 gcggtcaccg cttttgctca cgccaccaat caagtccgag gcgttctggg tttgtgccag
    20341 atattttgcc gcagtactgg ttgcgccttt ggttgagagc gcaaacaccg aggaaccgct
    20401 cggctgacga ctggtaaagg cgtcggcatg gatagagaca aacaggtcag cacgctgttt
    20461 ctgggctttt gctacgcgca cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta
    20521 caccttcata ttgccctctt tctcgatcag agagcgcaaa cggcgagcta tttgcaatac
    20581 cacatctttt tcgcgcgttt tgtatttccc caccgcaccg gaatcttcgc caccgtggcc
    20641 agggtcaagc ataatgacaa tcggacgatc gcgccctgct ttacccggtt gtggaccact
    20701 ttgcgctggc ggcacctgct tttcgaggtc gcctttgttg taatcctcca gcagcgccag
    20761 cagcgggtcc tgcatatcct gtgcattggc cggatagagg tccatcacca gacgctcttt
    20821 aaacccggcg accggcgcaa gggcaaacag ctgcggtttt acgttttgct ttaattcaaa
    20881 aaccatgcgt acggtttgcg ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc
    20941 gtctgcacga atttgtgccg ccatcccctt gagtaccgag ttcaggttta catcttcgat
    21001 atccaccacc acgcgttcag ggttactcaa cgcgaactgc ttatatttca gctgacgatt
    21061 tgattctacc gtcacgcggg tgtagctgga cgcaggccag acgcgcaccg ccacgacctg
    21121 gctaaccgca gccaggctga cctgacttac gctcaatagc cacatagcgc ccgcgccttg
    21181 cagtaaacga cgacggctga ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag
    21241 aaatctaagc tgtgtaacaa gtaaacgact aatttgaccg gaaactttag cgaaagacgc
    21301 ataatctgtc atctaataaa cggtaaacat tctttttata ttcacggcat tactgataaa
    21361 aaagtcgctc tcgcataaaa tttacacttg caccctgcga aaaaacagaa taaaaataca
    21421 ataatttcaa ataatcatgc aaaggggtgt gccgtggtaa aggaacgtaa aaccgagctg
    21481 gtcgagggat tccgccattc ggttccctat atcaataccc accggggaaa aacgtttgtc
    21541 atcatgctcg gcggtgaagc cattgagcat gagaatttct ccagtatcgt taatgatatc
    21601 gggttgttgc acagcctcgg catccgtctg gtggtggtct atggcgcacg tccgcagatc
    21661 gacgcaaatc tggctgcgca tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc
    21721 gacgccaaaa cactagaact ggtgaagcag gctgcgggaa cattgcaact ggatattact
    21781 gctcgcctgt caatgagtct caataacacg ccgctgcagg gcgcgcatat caacgtcgtt
    21841 agtggcaatt ttattattgc ccagccgctg ggcgtcgatg acggcgtgga ttactgccat
    21901 agcgggcgta tccggcggat tgatgaagac gcgatccatc gtcaactgga aagcggtgca
    21961 atagtgctaa tggggccggt cgctgtttca gtcactggcg agagctttaa tctgacctcg
    22021 gaagagattg ccactcaact agccatcaaa ctgaaagctg aaaagatgat tggtttttgc
    22081 tcttcccagg gcgttactaa tgacgacggt gatattgtct ccgaactttt ccctaacgaa
    22141 gcgcaagcac gggtagaagc ccaggaagag aaaggcgatt acaactccgg tacggtgcgc
    22201 tttttgcgtg gcgcagtgaa agcctgccgc agcggcgtgc gccgctgtca tttaatcagt
    22261 tatcaggaag atggcgcgct gttgcaagag ttgttctcac gcgacggtat cggtacgcag
    22321 attgtgatgg aaagcgccga gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt
    22381 ctggagttga ttcgcccgct ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag
    22441 ctggagatgg aaatcgacaa attcaccatt attcagcgcg ataacacgac tattgcctgc
    22501 gccgcgctct atccgttccc ggaagagaag attggggaaa tggcctgtgt ggcagttcac
    22561 ccggattacc gcagttcatc acggggcgag gttctgctgg aacgcattgc cgctcaggcg
    22621 aagcagagcg gcttaagcaa attgtttgtg ctgaccacgc gcagtattca ctggttccag
    22681 gaacgtggat ttaccccggt ggatattgat ttactgcccg agagcaaaaa gcagttgtac
    22741 aactaccagc gtaaatccaa agtgttgatg gcggatttag ggtaagcgat gaaaatcacc
    22801 ggatgcggca ggcgtaacac tcgtgcgtcg catccggcaa ttacgtttat tcccgcgaac
    22861 taaacaacgc cgccagacca ctgcgccgct cagtacgagt ggcgattgcc gcacttaata
    22921 tacgctcatc ggcatacagc gacagacgac gacgcgcgcg ggtcaccgcg gtgtatacca
    22981 gctctcgcgt tactaccggc gtgcgttggc tcggtaaaat caacgccgca tggtcgaact
    23041 ccgatccctg cgatttatgt accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac
    23101 tcggttgcac agacttaata ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc
    23161 cgcgatccag cgcaataccg atatcgccat taaacaaccc aagcgcgctg tcattacggg
    23221 caatcatcac cggtcggcct tcgtaccaac gagagtgcgg atgacgatga attttgcgct
    23281 tctgttgcat aaactgctca attcgctcat tcagtccagc cacgccaaac ggcccttccc
    23341 gcagggcgca caaaagctgg tactcattga acgcctgaat gattaaatcc ggctcggcac
    23401 gcgcttgcag cagatccaga taacgtccgt aacccgcaag agcttcctca agcatcgcaa
    23461 tataatcttc gccgctctgt aaaagtcgtt tttcgatatc agtaaaatcc tgctgaaaaa
    23521 cggttttcac tgccgtttta tcaccacggt tgatcgccgc agctaactga ccaatgccag
    23581 aatcgctgcc gaaacgatag cttttttgca gcaggcagag actgtcgcgc aaagatgccg
    23641 cttctgtgcc ggttcctgcc ggaacgtgag tccccgtcag gcggcttagc tgcctggcac
    23701 gctcggcggt aaagcccgcg ttggcataag cgcagatatc gcccagcaca gccccagcct
    23761 caaccgaggc cagttgatca cgatcgccga gaaagatcac tcgcgcatga tctggcaagg
    23821 cgtcgatcag tctcgacatc ataggcagat cgatcattga cgcttcatct accaccagca
    23881 catcaagatg cagcgggtta ccggcatgat gacgtaaacg ctggctaccc ggctgcgcgc
    23941 ccagcaatcg gtgcaaagtg ctggcatctt ccggaatgcg tttcttttgt tcatcggtca
    24001 gcggtaactg tcgcaaagcc ttgccgagag attcggttaa gcgcgcggca gctttacccg
    24061 ttggtgcagc cagacggata cggcagcgtt cgccgtcggc catttgaatt aacgctgcca
    24121 gcaacttcgc cacggtggtc gttttaccgg tgcctgggcc gccggaaatc accgagatcc
    24181 gccgcgtcag cgccactgcc gctgcaactt tttgccagtt aatttcatcg cttactggaa
    24241 aaagtttgtc cagggtttgc gccagtagag cttcatcaac ctcaatggta tgattcacct
    24301 cattgaagaa gcgcgctacc gtccgctcgt tacaccacat gcgattcaaa taaagacgat
    24361 cgccacagag gagcatcggc gtgggttcat ctcccctgct gaccgcttga gaagccagca
    24421 agcattcttc ccaattttgt agctcaccga tttcactgac acaggtcgcc aacagcggat
    24481 gcgacgcctc gttattttcc agtcgtgaaa gcggcaaaca gacgtgtccc tctccggcat
    24541 catgacttaa cagtgccgcc gcgagggtga cagcaggatg ttcatctccc gccacggtca
    24601 gggcaaattg cacatccagc gggcgtagct gtttgtgctc cacagcttcc agtaattgct
    24661 tttgcaattt cattacgcct cctccagggt cataccggca aacatctcat ccatcagggc
    24721 aatcaacccg gcgttgggtc gggttgtgta aatcccttgt tgcggatgtt ctttatcaac
    24781 gccacgcagg aacagataga taacgccgcc aaagtgaaga tcatagtcgt aatcagcaat
    24841 gcgatggcgc agataacgat gcagtgccag ggtataaagc tgatattgca gatcatagcg
    24901 gtgtgcctgc atcgctgctg ccatagcctg ttgggtgtaa gccgaactgt cttcacccag
    24961 ccagttggat ttatagtcga gcaggtaata acgcccttcg tggcggaata ccaggtcgat
    25021 aaagcctttt aacatgccgc gtacctgcat gaactccagc ggcgggcagc cagcggatag
    25081 cgggtcaaac tggcgaatta gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg
    25141 caggtaaaac tccatctcca cctgtttatc gcggtcggaa agctgactca ggctaacacc
    25201 cgtttcattg agaggtgcct ggaggacagc cgtgatccac tcggtcaaca ccggttccca
    25261 ctgcgattca aagccgccga gttccagttt ttcctgtacc cagttcgggt caaccggctg
    25321 ggtaaaatcg aggtcttcaa acaaactgtg caagaatgtc cccggtgacg caccgcgcgg
    25381 gaactgatgc ggtgttaacg tcggttcttc aacgacgctg accacgcctg cggcatcaac
    25441 atccagccgt ggcatcagat cctgagcgat accgtgacca cgctgctgca agccggagta
    25501 gctggtgaca cgccagttat cgccgggcaa tcgttgtagt gtcctcgcat tcagttctgc
    25561 agttaaagca tcattaacct gccagggctg gttatcacca gtttgtgccg tttgccaggc
    25621 aatatcatca tcgcataacg cttcaataca ggtgcgaagc cctgccgcat cttgcggttc
    25681 ccctttttgc agcaaacgcc cgagcgcact ttggtggacg tcggtgtcac cttttttatc
    25741 gccacgacgg cgcaccagcg gtgcaacgcc gagactgcaa tgccaaaccg aacgcgtcag
    25801 cgccacgtaa agcaaacgca gatcttccgc cagacgttcg acctccgcga ggtcgacgct
    25861 ttctggcgca gcattaagat ccaaaactgc ctcaaacgag tggcgatcgt gataaaacgc
    25921 ctgatcctgg acgcggaaat tggtaataaa tggcaaccag accagcggat attccagccc
    25981 tttcgattta tggatcgtga caatctgcac cagatgttta tcactttcga ggcgcatttg
    26041 ttggctggag gcattactgt ctggctcgag gatatgttgc gataaccagc gtaccagcgc
    26101 atgttcactt tccagctgcg ttccggcttc ttgaagcagt tcgctgatat gcaagatatc
    26161 ggtaagacgc cgctcaccgc ctgccgttgc cagcaagttt tctgcaatgt tacgcgccga
    26221 catcagcgcc cgcagcatcg gcataacgcc acgtttgcgc cagatttgcc gataaccatc
    26281 gaactcttcg actaccgcat cccacgcatg ttcgtcatta ttcagcgttt caatatccag
    26341 cgcattcagc cccatcattg acgttgccag cgcactgcgc agggtgttct cacgttcggg
    26401 cgtcatcacc gcctgcaaca accaaagcat ttcctgcgct tccagagttt caaaaacact
    26461 gtcgcggttc gaaaggtaaa cggaagggat ttccagcaac gttaaggcat cgcgcacctg
    26521 ggcggcctcc tggcggctgc gcaccagcac gctgatgtcc gaagcacgca ccggacgcgc
    26581 gtcgtcgtcg ttcatcagca acgcttcgcc ccgctgtccg gcttgtagcc agtcgcggat
    26641 ttgcgcagca catacctgcg ccatggtact ttgatagtcg ccaacgccgc agctttcgcc
    26701 ttccatcagc cacattttca tcgcaggctg cgtttcacct ttaaatacaa aacgtaacgc
    26761 ctgatttttc ccggctgatt tcactggaat aaacggtatt tcgcgaaaca tgaacgcgtc
    26821 atcagtctgg ctgaaaagct tgttcacact gttcaccatt ccaggtgcgg agcgccagtt
    26881 ggtgtctaaa gtgtagtggg cgtgaacttc actacgcgcc ttcatataag tgaagatatc
    26941 cgcaccccgg aatgcatata tggcctgctt cgggtcgcca atcagcaaca atgcggtttc
    27001 cggctgatgg tgccagatac ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa
    27061 ttcatcgatc attgccaccg ggaatcgcgt acggatcgcc gctgccaaca cctcgccgct
    27121 ttcgctacgc agcgcggaat cgagccgact taacatgtca tcaaaaccca attcgccacg
    27181 gcggcgtttt tcacgcgcta ctgtttcgcg gatctcagcc aatgctcggg tgatcaccag
    27241 atcgcggatc gacaatggtt ctgcaagcag ttgatcgatc acctcaaaca gtggatgtcg
    27301 cggggtttcc cccccggcct tcgtgcgatc ttctaagaaa cgctgggaga atttttccag
    27361 cgactccggc aactgataac tgtttgtctc ttcttctgcc caagcgctga tcttttcgat
    27421 ccatttagcc tgattgctac ggttaaactt gcgtcgatca ataccagaag attcgatcag
    27481 cgcatccagt tcacccactg cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac
    27541 aatttgcgcg tggcgggaag ccagcgtttc atcatcgggc ggcggtgctt tgataaccgg
    27601 cgcttcgcct tgcagataac gattaatatc gcgcagcaac gcctgcggcc ctttccaggt
    27661 ttcaaagacg acctgggcta tttcacgcgg cagcgggtag cagtggcgac gccagaaatc
    27721 ggcgcaggcc tggtagcgta gcagagactc atcttcaatc agctgctgct caaacagcat
    27781 gccggattca aaggcattca ggttgagcat gcgctggcaa aagccgtgaa tagtaaagac
    27841 tgccgcttca tccatctgcc gttcggctaa caacaaccac tgcgcggctt gcgctttatc
    27901 gtcgatctct tccagcaggc gttcgtacag tggattgtcg gtggtttcac gcagacaggc
    27961 gatgcgcaac tcgtggatat tgctacggat acgaccgcgc aattctgccg tggcagcctc
    28021 ggtaaaggtg accaccagca gttcttcaac gatcagcggg cggggaaagg cggcggaacc
    28081 gcctagtcca agtaacaggc gcaaatagag cgccgcaatc gtaaaggttt tgcctgtgcc
    28141 ggcagaggct tcaatcaggc gctcgccctg taagggcaag cgcaaaggat ctagtgtctc
    28201 ggcgacatca ctcattcttt tcactcatca ggggcattgt ttgctgcaac gcgctgacgt
    28261 tctcccacac tttccagcct tcaggatgca catattcggc tttcccgttc tggctgccgg
    28321 aaatctgcga cagaatagcc atgccttgcg gctcgaccac cgcctgatgg aagaaatcag
    28381 caagtttttg cggcgtcagc agttttatct gggccacgat tttatcacgc gaatcgaagc
    28441 gcatattgcc gcgatcgaaa tctttactta acttcgatgc ttcttcgccg agcgtttgcg
    28501 gtgcctgcag catctgggta attaccgcct gctggatttg cgcaaactca tctggcttca
    28561 tcgctcgcaa ttttgcctct gcggttggga aaaacgcctt gtaacgctcc cacaagaatg
    28621 aaggctgttt atcattgctt tgcaaaagga agcccatgcc ccactgacgc cccacgctca
    28681 ttggaaacgc aaacacggca tagcccaatt gttcttcggt acgcaactga ttgtagaacc
    28741 acggctgtac gatctgcccc aacagagagc tataggctga gctggtgtat tcatcgtagc
    28801 cagtcggtac aaataccgct gccagtgcgg agtcggtgct gttaccggct ttttcaaaga
    28861 tgacggattg ttttttatcg accactacat ctttgtttcg acaccactct gaaccatcag
    28921 cgcccaactg tttttgcaca tcgcgtgcca gcgttgttgc ctgggcctcg gtcatgttgc
    28981 cgataaccat aaactctggt cgagcccctg attttaaggc gtcgcgatag gccagcacct
    29041 ctttcaacgt aatggagggc aaaattttac gccgttcatc tcgcgagaag tacggcactt
    29101 gcgagagcat ctgcgcgggc ataatcgcct gctcaaacgc tttacccttt tctgcggaat
    29161 ccatcatctg gttataccag gacttcgcct gctcaagctg ctcttccgta gcggtatagc
    29221 taaagtaccc ctcgagcaat gcctggaaca gctgcggcag acgctgggtg taaccattag
    29281 cattaaccat aaggccgttg ttagcgttgg tggaaaaact tatgccacca accgacgcct
    29341 ggttgcttaa ctgatcaagc gccagccctg cgagataatc attgagcgca aacatcacct
    29401 gattgcgggc gctgtccatg gctttcggat tacgcaaaat caggctgaca tcagctttgg
    29461 gctcgctggc aaaataacgg cttggcgcat acaccacgcg cagattcgac tcatcaacaa
    29521 tcagctctgg atggtcgtat ttcttctctg acttaatcag cgagaaatca tcaggaatat
    29581 aagggttaag ctctggcaaa gagagcgcaa tgtcggcggc ttttttctgc cagtcggcga
    29641 aagtttgtgc gctgatttta tcgacctgat acggcgcatc gacaaagtaa gccgttttgt
    29701 tgtgcggctc tttcgggctg atataccaga tacgcgcatt ctgcggcgtc atcatcgcca
    29761 gacgttcctt tactgcttta gcatcgtacc gatcggcaat attgactgca tccagcgtat
    29821 gctcaacagg aacgcgaatc atggtatctg ccagccattc gacgtaatcc atatcacggg
    29881 tgatcgacgg ataacggaag tcgatatcca gcacattcgc cagttcatcg aagtattgtt
    29941 tatcaatgcc tttttcacgt aacagattga gatagctaaa aattgccgcc acaacctgat
    30001 cgcgattagc caggccttta tcagttaaag acgcagagat cgctaatacg ccgctgttgc
    30061 cgttgacgat aggatcggag ttggcgctaa tgccctcaac taatccctgc ttttgcagcc
    30121 agtcagaaag tgtacctggg ctgcgattgc caatcagata ggtaatcaat tcatcggttt
    30181 tactacggaa cttcgctgag ttgttatcga tgcgaaactc aacgcgcaac actttacgcg
    30241 gcagcgcagg gacgtaatga atgataatgc ccttttgcgc gtcggtgact accggcacgg
    30301 tgatttccgt ttttttgctc tctttgttcg gcacgcgacc aaaggtgtcc gccgccattt
    30361 ttgccaactc cggcagcggt ttattactgt aaataaccgc cttcatcaaa ttggcggaat
    30421 agtacttctc gtggaaatct ttcagcgcct gctgcaccgg attaccaggt ttgtcgctta
    30481 aagtttcgag gttaccacca gaaaactttg aaccggggtg tgccgggtta atggtttctg
    30541 cactgacctg tgccatgcgc atcccgtcac gcgtacgcgc catggttaat tcagcgttca
    30601 ctgcattacg ctcacgttcg gcgtatttct tgtcgagcaa gggttctgca atagcatcgg
    30661 ccaggcggtc taccgcaccg ggcaaggcgt cgttctcaac ttccagatag aaagccgtgc
    30721 gatacggcgc agtgctggca ttgtgactac cgccgtgcat tttgagatat tcggccagac
    30781 tgtcagcctg cgggtacttt ttcgacccca tcagactcat atgttcaagg taatgtgcca
    30841 gcccctggta cgcctcggga tcttccagcg acccaacggg cactaccagc gccgagagcg
    30901 atttaactgc ctgcggatca gaaaccagca agaccaccat accgttatcc agacgtatag
    30961 cctgatactg gcggttatct ttatcacttt tacggatggt ttcctgaatc ggctgccatc
    31021 ccgtttctgc ctgacttaat ggtgcccaga gggcaacaaa caacaataat gctttgaacc
    31081 aggtgctgcg gggcattcac ggacctcata atcaacttta ttttctggac agattcaaca
    31141 ggttaacgca aatcatctgc cagaatttaa tcttgtgctg cacgagtcag cctatgttta
    31201 tataaccatc agtccgtgac tggtgcgcat cataaagtaa gcggatagat tgcgcaattt
    31261 ttatacaaca ctcatgactg attaaagcga aacagcggta acaggaaacg ttgcgactgt
    31321 tcaacgatgg cctccattgt ctctggtgtt aattgccgcc agagccgttg ataccagata
    31381 tcatcacctt cgccacgcac catcatgttg ccttcgtaag cctgaaggaa tttcgtacgg
    31441 gctttttgca acgtggaatc gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt
    31501 tttagccacg cgccgccact ttcaggtaac accagcaatg gcgcggacat tccttcacga
    31561 tacccctcaa tcagttgtga gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa
    31621 cgccactcac cgtctttgcg tagaaaaagg cgactttcgc cattaccacc gctggcacag
    31681 tagacaaggt gttccagcca aagttgcatt ccctgcgcca cacttaataa agagggacgc
    31741 cagcgcaaca ggccatccgg ctgcacctgc ggcaaccagc cagttatctg cacaccgttg
    31801 caggcgagat caatttccat actctgcccc ggctggcgac aggcaatgac tctgtcggca
    31861 agctgctgca tctcctggca ctgtgtttcc cagaaaattt caccaaaagc gccatacggt
    31921 aaatcccctg ccgcccggaa gcggcggaac aagcgttcgg catcatcctg ctcaaccagt
    31981 gcattcaata actgctgatt gatttgataa cggctaagtc cttccagaat aaatggctcg
    32041 gtgtctggga tttcgctgtc ttcagtacgg aagttcacct gcaaacgcat ctggaagaac
    32101 gcccgcaccg gatgtgccca gaatcgttgt agcgtttcca gcggcacggt ttccggtaag
    32161 gtaaacggca gcggctgaac aaattcagaa tgtgctttac cagcctggct ggccgcaggt
    32221 agccattcac gagcatagct ttgtcgttcg cctggctggt agttttgcgg atcaaacggc
    32281 atccgggtat ggaggcaagt aagatgcgct tttacccttg cctcgctttc atcacagttg
    32341 agcgcttcat cgcccggtag ataatgactt tgcccgatgt agtcgatcag ttcctgcacc
    32401 agtaccgacg ggaaacgttc actgttatcc tgaatggaac gcccgatata gctgatatag
    32461 agtttttgct gcgcggaaat taacgcttcc aggaacaggt agcggtcgtc atcgcgacgg
    32521 ctacggtcgc cacgcttcgg tttctggctc atcaggtcaa agcccaatgg cgcaagctga
    32581 cgtggataaa cgccgtcgtt cattcccagc aggcaaacca ctttgaacgg aattgaacgc
    32641 attggcatca gagtacaaat attaaccggt ccggcgagaa aacgctggct gatacgttct
    32701 tgatccagac gctgtgccag ttcatcacgc aatagtgaca gcggcaccgc gtcgccatac
    32761 tgcgcaccta aaccttcggc gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc
    32821 gcttcggttt ccgcatccgg caggaagaag gcgttgagca tatcgcgaca aaccggcaac
    32881 cactcttcta gcggacgctc ctgtgccagc ccgcgacgcc agatgtttag ctgcattagc
    32941 agtgaagcca gatgccccac cagttctgca attaagccgc tcgattcatc ataaggtaga
    33001 accgattgcc actcgccctg cgcgctctcc atcgcgtagc ccagcaacat acgcgtcagg
    33061 ccaaatcgcc aggtgtgttg tccggtggcg gggagttcca gctcgcgaac gttgtcgtca
    33121 tctatccccc aacgaatgcc ggattcgttg acccactggc gtaaataacg cagcccttct
    33181 tcggtgatgt caaaccgcgc cgccagcacc ggcacatcca gcagcgccag cacatcctct
    33241 gacacaaagc gactgtcagg cagtgataac aggctgataa acgcttccag taccggatgt
    33301 gactgccgcg cacgacggtc ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta
    33361 ccaaacactg cctgaataaa cggactgtag ctgtcgatat cagccaccat cacgatgatg
    33421 tcgcgcggag taagtgtcgg ggcttcctcc agcatcgcca gcaagcgatc gtgtaaaact
    33481 tcaacttcac gctgcgggct atggcaaacg tggaatgtga tactgctatc cagtggatca
    33541 agcgggcgtt tgttatcgct acgggaaaac tcttcgatgt tcacacccgc aacggcgcgg
    33601 ttttccagtt ccagaatgtc agactgaatg ttatgcagca ggttatctgg cgtcacatcg
    33661 acaaaagcgt ccagctcctg gctgctctcc aggtcagaaa ggagataaat gtagtcgcgc
    33721 ccaagcttcc cccatgaggc cagcagcggg ttgccgacat cctgttcacc atcgctgtta
    33781 aagagctgtc cggcattttc gctatcgcga aatagcggca attcgcgatc ttcaaaactg
    33841 tgtcgacgct gacgagtcag cagtttcgcc agataagcag gatctttaat atcgccccag
    33901 taataacggc aggggttggt aaacaggaga tggatttcaa tatgtttacc cagcgcctgg
    33961 agcgcctgga gataaacagg cggtaacgcg gaaataccgc atataaagac gcgcgaaggt
    34021 aaccccggcg ggcaggtcgt cgcggactcc agcgtttcga taaagcgctg atagagattc
    34081 gcgcggtgcc agcgcggttg tccgagttca tgggtatatt ccaccagcgc cttccacaac
    34141 ggggcttgcc aggcctgtgc ttctcccagc ccttcaacca aatgtcctgt ttcccactgt
    34201 gccagccagt ccggacgata gaccagatac tggtcaaaca ggtccgccgc ttttgaggaa
    34261 agctggaaca gttttcgctt gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag
    34321 tcttcgcgct ccagcaattg cggcagcaga gtcatcagtt tccagctcat gctctgtttg
    34381 ttaaaggcgc tctctttggg gatttccggt aacacccgga cgaacatatc ccagataaag
    34441 ctcgctggca gcggaaaatc aatgtttgcc gcaataccaa acttttgcga cagggtcatt
    34501 tgcagccact gcgccatacc ggtactttgc accagaatca tctctggttc gaaaggatcg
    34561 tccagccgtt cgcgttcgac aataaactcc atcaacgctt ccagcacgtc cagacgattg
    34621 gaatggtaga cccttaacat agcggctcct gactactgac gattcgggca atgcaggcgg
    34681 gtcatctcgc cttctctgcc cccgggtgaa actagcgtaa cgctgatgct gacacatccc
    34741 gcctgcgatg tctgcattcg gttgacctgc cagttggcag gtggcgaaat cgcgcgcagt
    34801 tgcgtttgct gccaaccatg ccgccagagc tgttggtact ggtttctgct ggcgagactg
    34861 ttcattaatg ttcgctggat acccgataac gcagtgacaa tcatcaccat cagcaccatc
    34921 gcaaccatta cctccggcag gctaaagcct tgttgattct tcagggaagc tgacataatg
    34981 ccccctcttt cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca atttttccat
    35041 caatgacttc gcctgttcgc cacaacgaaa cgccttcata accggcaatc aataaggctt
    35101 cattatctgc cagtaaacgc aaacaaacct gggcatcggt tccagcgtac tgcgagcact
    35161 gaactgctgg ctgcgtctgc caggagtgca tttttcccca cgccagcgcc gactgaacga
    35221 tggcctggcg gcgcaatgac tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct
    35281 gctgactcat tccttgcaat agcaagctac ccaaaaccag cagcatcagg accagagcca
    35341 gtgacgaaac acctttttcg cggttcacag gttgaatcct gtcacgctat agctggcatc
    35401 caccacggtt tgcggttcag acttactggc agcacgcata ttaaccgtca acaccggcga
    35461 gaagccgctg acatcctgac gtacgacctg aaaagtgtcg ataatgatgg catccggatt
    35521 agtgacttta tcccagccct taccttcaca ggatgtcgca ccgcgtagcg tttccagcac
    35581 atgctccttc agacgaaatc caatctggtc ggactctttt accggttcgc gatcccagat
    35641 accgttactg ttcgcatccc actgcacaat gatacagtca ccctgtccga caatttccag
    35701 cccttcgccg gtacagctgc catgacaata acccgccctc tggagatgct tcgcgacggt
    35761 aaatacccgc agccagattt catcttccag tgccagctta cgagtgctcg ttaaactttc
    35821 acgctgtaac gcaggcagaa agcgtgcagc ccccagcaac aatacgctac tgatcgccat
    35881 agcaatcaac acttccagca gagaaaaacc ttgctctttt acaggcatcc ttctgtttct
    35941 ccttgctgat aaagccggag tcttccccac ggcgaaacca ccagccacca ctcgcccgtt
    36001 gagtttttga agcgaatatg cccggcccat gcggtattgc gcaggccaaa gaaagcaagc
    36061 gaaggtgtca ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa
    36121 ctgccatgac aggtattggc cccagcagcg gaactcacaa ggcaccataa cgtcccctcc
    36181 ctgataacgc tgatactgtg gtcgcggtta tgccagttgg catcttcacg taaatagagc
    36241 aaatagtccc gcgcctagct ggcggtttgc caaagccgtt gcgactgctg ccagtattgc
    36301 cagccataga gtccacttgc gcttagcatg accaaaatca gcatcgcgac cagcgtttca
    36361 atcagcgtat aaccacgttg tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga
    36421 cgagggccag tttctatttc ttcggcgcat cttccggact atttacgccg ttgcaggacg
    36481 ttgcaaaatt tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa accgacgcac
    36541 actggcgtcg gctctggcag tatgtttcgt aattagatag ccaccggcgc tttaatgccc
    36601 ggatgtggat cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg
    36661 ggtttacgtt tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga
    36721 gtttgatcca tatggttgct gtacaggtgc gtatcgccac cggtccagac aaaatcaccc
    36781 acttccagat cgcactgctg cgccatcata tgcaccagta acgcgtagct ggcaatgttg
    36841 aacggcaggc cgaggaagac gtcacaggag cgctgataaa gctggcaaga gagtttgccg
    36901 tctgccacat agaactggaa gaatgcatgg cacggtgcca gcgccatttt atccagttcg
    36961 cctacgttcc acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt cagctggttc
    37021 agtaccgtag taatctggtc aatatgacga ccatctggcg ttggccaggc gcgccactgt
    37081 ttaccataca ctggcccgag gtcgccattt tcatcggccc attcgtccca gatggtgaca
    37141 ttgttttcgt gtagataagc aatgttagta tcgccctgca ggaaccacag cagttcgtgg
    37201 atgatggaac gcaggtggca acgtttggtt gtcaccagcg ggaatccatc ctgcaggtta
    37261 aaacgcatct gatgaccaaa aatggaaagc gttccggttc cggtacggtc gtttttctgt
    37321 gtgccttcgt cgagcacttt ttgcatcagt tctaaatact gtttcatggt tcctcaggaa
    37381 acgtgttgct gtgggctgcg acgatatgcc cagaccatca tgatcacacc cgcgacaatc
    37441 atcgggatgg aaagaatttg ccccatgctg atgtactgca cccaggcacc ggtaaactgc
    37501 gcgtcgggct ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac
    37561 aagcctgaga cagctcccat tgggcgcggt ttacgaatat acaggttgag gataataaac
    37621 agcaccacac cttccagcag cagttcgtaa agctgtgatg ggtggcgcgg cagcacaccg
    37681 taagtgtcga aaatggattg ccactgcggg ttggtttgca gcagcaaaat atcttctgta
    37741 cgggagcctg ggaacagcat ggcaaacggg aagttcgggt caacgcggcc ccacaattca
    37801 ccgttaataa agttgcccag acgcccggca ccaagaccaa acggaatgag tggtgcgata
    37861 aaatcagaga cctggaagaa ggaacgttta gtacggcggg cgaagataat catcaccacg
    37921 ataacgccaa tcaggccgcc gtggaaagac atgccgccgt cccagacacg gaacagatac
    37981 agcggatcgg ccataaactg cgggaaattg tagaacagaa cataaccaat acgtcccccg
    38041 aggaagacgc cgaggaagcc cgcatagagt aagttttcaa cttcattttt ggtccagcca
    38101 ctgcccggac gattcgcccg acgtgttgcc agccacattg caaaaatgaa acccaccaga
    38161 tacatcaggc cgtaccagtg aagcgccacg ggtcctattg agaaaatgac cggatcaaac
    38221 tccggaaaat gcagatagct actggtcatc tgtcaccaca agttcttgtt atttcgctga
    38281 aagagaacag cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat
    38341 agcacaaggg gacctggctg gttgccggat accgttaaaa gatatgtata tgatccgcgc
    38401 tataaccctc cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc tgcaacctga
    38461 tgacgaactt cggtcgccag ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg
    38521 gcaaaatcaa tgcgccgcag caggtatttt acccgcgcta cagaacgtcc gttcatagac
    38581 agatggcgat acccaagccc aatgaggatt gccacgcaca tgggatcgcc cgccatttca
    38641 ccgcacaaac ggagatcgat accatgtatt tccgcttccc gggcgatcat cgccagagct
    38701 cgtaacattg caggatgaag actgtcgtaa atgttcgcca cccgggtatt gttgcgatca
    38761 acggccagaa tgtattgagt cagatcgttg gtgccaacag agatgaaatc gacccgcttt
    38821 gccagatgcg gcagcataaa taccattgac ggcacttcca gcatgatgcc gatacgtggt
    38881 ttgggaattt cgtaaccgat catctcctcg acttcacgtc cggcacgttc aatcaggcgg
    38941 cgtgcttcgt caacttcatc gaggctggtg accatcggca acagaatatt caggttgccc
    39001 gtagcggcat tagcacgcag catcgcccgc acctggatca agaagatctc cggctgatcg
    39061 agcgtaatgc gaataccacg ccaacccagg catggattct cttcgctgat cggcatgtaa
    39121 ggcagctgct tatctgctcc gacatccagc gtacgcaagg tgacgggttt atcattgaac
    39181 atttgcagca tcccctgata ctgcgccacc tgttcttctt ccgacgggaa accactttgc
    39241 agcatgaatg ggatttcagt gcgataaaga ccgatgccat caatacggct gcccagtttt
    39301 tcttcatgtt ccgggcttaa accagcattg agcatgactt ttatacgctc accgcttttt
    39361 aactgcgcgg gtaaattgac gtcatcttcc gccagacggc taagctcaat ctcttcacta
    39421 attagccgct gatattcttg cagcagtacc ggctccggat cgaccagcaa ttcaccgcga
    39481 tagccatcaa cgatcagcgt cctacgatgc agcaccgaag gctgaatatc cgcgcccatc
    39541 acggtaggga tccccagcgc acgtaccatg atcgcagcat gggagttggc tgcgccatct
    39601 cgcacgacaa caccgactaa gcgatcctgg ggcagctcag caagagttgt cgctgacagt
    39661 tcatctgcca ccagaatgaa acgttccggc caggcgttcg gcccttgatt agcgtcatca
    39721 agatgaaaca gcaatcgctg acccagcgca cgtaaatcgc cagcccgctc tttgagatag
    39781 ttatcgctta gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt taccgcccac
    39841 tctgccaccg agcctttatc aacctcggca aacaattcgc gacgcagccg ggtgtccgaa
    39901 agcaggtgcg agtaaagatc gaaaatagcc gccatttctt tttgtgctcc ggcggcaaag
    39961 cgtttgctgt agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg
    40021 cgttccagag ccggatccag cgttgatgcc tgatacacct gttccattaa aggtaacgtg
    40081 gcatcctgcc agccttcggc aatcgccaca ccaggtgctg ccggtaatgc gcggattcgc
    40141 gtctggcgat attgcccaaa cagggcagtc aactgcgact gagaaagaat agctgccatc
    40201 tgggtggcaa gcgtcaccag gaaggattct tcactttcgt catactggcg caactctcgt
    40261 tgctgtacca ccagtacacc aagcaactgg cgacgttgaa taattggtac gcctaaaaac
    40321 gcgcggaaac gttcttcttt tatggagggg atgtatttga agctggggtg cttttgcgca
    40381 tctgcaaggt ttatcggttc cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac
    40441 gcgagcgtta cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat caggtagtaa
    40501 caacgtcgat catgatcggc caggtagacc gaacagacct cggtatccat cgcaagacag
    40561 atgtcggtaa ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc taccttttcg
    40621 actatttcgc gcaggcgagt gagcataatt ggcgtgactt aacctctttt acgtcgataa
    40681 gcagatgcgt tttgtggttt tggcgtattt tcctgcagtg acatcaccac actcgcgaac
    40741 tctttcatta ccctacggta gacatcacgt ttaaatgaca ccacctgtct gaccggatac
    40801 cagtaactta cccatcgcca gccgtcaaac tctggtgtac tgctggtttg catattgatt
    40861 tctgcatcgc cgcttaccag ctgcaagaga aaccattttt gtttttggcc gatacaaacc
    40921 ggcttcgtgt cccaacgcac caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt
    40981 gaagcaagga ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa ttcacggtac
    41041 atcgcctgct ctgcggattc tccggggttg attccgcctt gcggaaattg ccaggagtgc
    41101 tgaccaaatc gccgggccca cattacctgc ccctggcgat tacaaatcac gatacctacg
    41161 tttgggcggt agccatcgtc atcaatcacc ggactacctc aaaataaagc tttatatacg
    41221 aatgattgtt tcatactcca ggaagacggt aaaccactct ctgcagggca ttacacacta
    41281 ataacaattg aataactcac agttatgtgc agagttataa acagaggaag gggtggatag
    41341 ccgtttttat tcaccttttc tgtggataga gttgtgaaga actacggaat tactacggga
    41401 aaacccggag cattctgaat aagcccgata tagacatttt aaaatattca ttattagtca
    41461 atgcattaca tgtaatttga gtaatgaaaa agagtaattc gtgacccagg tcacacctct
    41521 catttacggg ttggcgaaag atcaaccaat gccgtattta tccacagaat gtgccactaa
    41581 gttaagcact gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg taaatgaaaa
    41641 cagtagtgga ggtttttcac agttatccca gctttctgtg gataacatgg tgtaagatcc
    41701 tgtttatttt cagtgaccag atttggaaaa cctattgcag tgttgcgcaa ctcgattacc
    41761 ggcagcctaa aaagcaatat aaatcagaga attgaacaac acatgtggaa aaagttacac
    41821 tgcgaatatt cggcacataa ttgctgtttg ttttttaacc aaggcatcat gacatgtccc
    41881 aacctcgccc actgctctct cctcccgaaa ctgaagaaca gttgttagcg caagcacagc
    41941 aactttctgg ttatacattg ggagaactgg cggcacttgc cgggctggtt acgccagaga
    42001 atttaaaacg cgataagggc tggattggcg tgttactgga gatctggcta ggtgccagcg
    42061 cagggagtaa acctgagcaa gattttgctg ctctgggcgt ggaacttaaa actatccctg
    42121 tggatagtct tggtcgtccg ctggaaacaa cattcgtttg tgttgccccg ttaacgggta
    42181 atagcggggt gacctgggaa accagccatg tgcgccacaa gctcaaacgc gtactgtgga
    42241 taccggttga aggcgagcgc agcatcccgc tggcgcagcg tcgcgtagga tcaccgttgc
    42301 tgtggagccc gaatgaagag gaagaccgac agctacgcga agactgggaa gaattaatgg
    42361 atatgattgt tctcggtcag gttgagcgga tcaccgctcg tcacggggaa tatttacaga
    42421 taagaccgaa agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc cggggcgaac
    42481 ggattctgac gctgccacgc ggcttttatt tgaagaagaa tttcaccagt gcactactgg
    42541 cccgtcattt tctgatccag tagccatcgc tttgacctgc cgctttccgg gcatataatt
    42601 accgcttcat ttttttggca gggcttttta gatgttattt gcatggataa ccgatcctaa
    42661 cgcctggctt gcgctcggta cgctgacgct gctggagatc gttcttggga tcgacaatat
    42721 tattttcctt tctctggtgg tggcaaagct tcccacagca caacgtgctc atgcgcgccg
    42781 tctggggttg gcgggagcca tggttatgcg tctggcgctg ctggcatcaa tctcttgggt
    42841 tacgcgcctg acaaatccgc tttttacaat attcagtcag gaaatttctg cccgtgattt
    42901 gattctgctt ctgggtggct tgttccttat ctggaaagcc agcaaggaaa tccacgaatc
    42961 cattgaaggt gaagaagaag ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt
    43021 gcagattatg ctgctggata ttatcttcag cctcgactcg gtgattaccg ctgtgggtct
    43081 gtcagatcac ctgtttatta tgatggcagc cgtggtgatt gccgtaggcg tgatgatgtt
    43141 cgccgcgcgc tcgattggtg attttgtcga acgccatcct tcggtaaaaa tgctggcgct
    43201 ctctttcctg attctggtgg gctttaccct gattctggaa agtttcgaca tccacgtacc
    43261 gaaaggttac atctacttcg cgatgttctt ctctattgcg gttgaaagcc tcaacttgat
    43321 tcgcaacaaa aagaatccgc tctgatactc cgtacgctct cctgcgggag ggcgtactca
    43381 ctccttttac cctcacagtt taagattttc ctgctttcaa gatatataac gtcgatttat
    43441 aaacagacta ttatcatagg taggccaaat gaggataacc ggatgaaaaa atgggcagta
    43501 ataatttctg cagtcggact ggcgtttgct gtttccgggt gttccagtga ttacgtcatg
    43561 gcgaccaaag atggccgtat gattttgacc gatggaaaac ctgaaattga tgatgatacc
    43621 gggctggtga gttatcacga tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt
    43681 tcgcaaatta ttgaacgtta acaaataagg tcagcatccg gctggcctta agatttttct
    43741 ctttcccttt tcccttccct ctgccatttt tatattcctt atgtcgtgat tataaaaagg
    43801 aaacggctat gcaatatcac cgtatacccc acagttcgct ggaagtcagc acgctggggc
    43861 ttggcacgat gacgtttggt gaacagaaca gcgaagccga cgcccacgca caactcgact
    43921 atgccgtcgc tcagggcatt aaccttatcg acgttgccga aatgtaccca gtacctccgc
    43981 gccccgaaac gcaagggtta accgaaacct acgtcggcaa ctggctggcg aaacatggca
    44041 gccgcgaaaa gttaattatc gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg
    44101 gcatccgccc ggatcaggcg ctggatcgga agaatatccg cgaagcgctg catgacagcc
    44161 tcaagcgcct acagactgat tacctcgatc tttatcaggt gcactggccg cagcgcccga
    44221 ccaactgctt cggcaaactc ggttatagct ggacggattc tgcgcctgcg gtttcgctgc
    44281 tggatacgct ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg
    44341 tgtcgaacga aactgcattt ggcgtaatgc gctacctgca tctggcggac aaacacgatc
    44401 tgccgcgtat tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt tttgaagtag
    44461 gtctggcaga agtcagccag tatgaagggg tcgaactgct ggcctattcg tgcctgggtt
    44521 tcggcacgct gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc
    44581 tctttagtcg gttcacccgc tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg
    44641 ttgatatcgc cagacgtcat ggccttgatc ctgctcagat ggcgctcgcg tttgttcgcc
    44701 gtcaaccgtt tgttgccagc actctgctgg gcgcaaccac gatggatcag ctgaaaacta
    44761 acatcgaaag tttgcatctg gagttaagcg aagacgtatt agctgaaatt gaagcggtac
    44821 atcaggttta tacttatcct gcaccataaa gattaacgcc ggttaaaacc ggcgttaaat
    44881 attaatgacg gcgctgccag atccacagcg ccgttattgc cagcgcaaat agcgcaccga
    44941 agccaatgcc aatgggcacg accgggatgc ctaccattac cgccagcgag taaatgccca
    45001 gcatcaacaa catggcgctg ttttcgccaa ggttttgtac tgcaatcgca ttccccgccc
    45061 cgacgctttt tttaccccgc tcctgtagca acgcattgag cggaacgaca aaaaagcccc
    45121 ccagcacgcc aatcagcatc aacaaggcat aggctggcag cagctcgtgt tgcagggaga
    45181 aaatcagtac caccacgcca atcaaaatcc cggctggcat acagcgtgac acggtttcca
    45241 gcgtaaccag cttcgctgcc gcacctgcgc caaccacgat accaatcgct accatcgcgt
    45301 tgagataggt cggcgtagcg ttatcggtaa tgcccagcgc caccggtacc cacagcacca
    45361 acaggaaacg cagcgtgaca cccgctcccc agaataaact ggtgcccacc agcgaaaaac
    45421 gcgtttcacc attgcaccat agcgaggtgc aggcattcag gaaactgcgg gtcatgttga
    45481 tgagattcca ggactgcccc ggacgcgccg ccgccagttt gggaatgtag atattggcaa
    45541 cgaccgcacc accgtaggcc agtacgcatg cggccagggc gaccaggaca tgccagtcag
    45601 ccagcacacc accggctacg gaaccgagta aaatcgccgc tatggcagaa gcttccatta
    45661 aaccgttagc ttttactaac ttactacccg tggttaattc gccgagaata ccgtatttcg
    45721 ccggtgaata ggctgcagca ccaacaccca ccagcgtata gccgagaaac ggattgatac
    45781 caaagcagat actggctgcg cccagcagct tcaggccgtt ggcaaacatc atcacccggc
    45841 ctttggcgaa gctatccgcc acctgcccga caaacggcgc caaaagaatg taagcaccta
    45901 caaacaccat ttgcaggatg ggctggctcc actccggaca gaactgcgct ttcagtaacg
    45961 ccagagtggc aaacagtagc gcgttatcgc caaacgcaga gagaaactgc gccacgataa
    46021 ccgctttcat ccccttcgac cacaacgaag tgttagtgtg cactgactca ctcatcgtgt
    46081 tgttccgctt cgtctaccca gcttttcaac gtgacaaagt caggtttgcc gctgccaagt
    46141 aatggcatct gtttcagata gcgaatatcg cgcggtacag caagctccgg cacgccgtgc
    46201 tcgcgggcat actgttgcaa cttatcgcgc gtcagttcgt tatctgtggt gaaaagcacc
    46261 agtgcctcgc ctttgctggc atcgctctta atcgcagtgg catggacttt atctggcgaa
    46321 acaccaagtg ccagttgttc caccatttcc agcgacacca tttcgcctgc aattttggca
    46381 aagcgttttg cgcggccctg aatctgcaca aagccctgct cgtcaaaacg cacaatatcg
    46441 ccagtgtcat accagccgcg ctccatttcg ccgcgaacat tctcggcggt gggcacttcc
    46501 agtacacctg gcttctccac ccgcagatag ccgttcatta tgttcggccc tttcagttgc
    46561 aggcgtccgc cctcttcgat accagggacc gacaacaggc gtgcatccat tcctggtaga
    46621 atacgcccta ccgtaccggg tttcgccgcc atcggtacgt tgatagaaac gacaggcgcg
    46681 cattcggtca caccgtagcc ttcaaggatg cgcaggccaa atttatcctg ccaaagctgt
    46741 ttggtacttt cttgtagttt ttctgcgcct gccaccacat agcgtagacg atagaagtca
    46801 tacgggttgg cgaaacgcgc gtagtgaccg aggaaagtcg aggtaccgaa caacacggtg
    46861 caactgcggt catacaccaa gtccggcaca atgcggtaat gcagcgggct tggataaagg
    46921 aacacttctg cacctgtaag cagtggcgta aacaggccta ccgtcagccc aaaggagtga
    46981 aacagcggta atgccgacat aaagcgatcg ttggtggtga agtcggcaat cgttttaatc
    47041 tgctcgacgt tcgccagaat gcttttatgg ctatggacga cgccttttgg atggccttca
    47101 gaaccggagg taaagaggat cagcgcctct tcttccggct gctgttttac ctgcgccagg
    47161 cgcggcatca gcaaatgagc gaagatccat actttgtcgg cagtggtgac atctgctttt
    47221 aaatcttcca gatagaccca gcgcacctga gtaagttgct ccggcagatg ccagagtttg
    47281 cctttatcga gaaactggcg ggaagtgaag atggttttga tttcagccgc cgtaataaca
    47341 ctggtcagcc cttttacccc ggcagtgtag ttcatcattg cgggaatgcg gcgacgcgcg
    47401 atggccccaa aaatcactgc cgcactgatg cctgcattgg gcagcattaa gccaatgcgt
    47461 tcgccttcaa cactgtattt ttcaaggatg cgtccaacaa acagcgtttt cgtaagcaat
    47521 ttgcgatagg agtctggggt aaagttgacg tcttcgacac atttcttccc ggctccgaag
    47581 cggtacattg cactcagtaa agattcgtac agagtttcac gcgggcgcac cgccatacgc
    47641 gcttccatca ttatttgatg cagcatttcg ccagcgattt tgcgacggtc acgggcacgc
    47701 ggcgcatccg gcatcgccac ctgcgttgga ggcaaaatat gcagagtaat ttgcgggaac
    47761 aagcgacgtt taaccagacc tttcaggcgg ctgaagtgcg taagttccgc cccttcaata
    47821 cgcacaggaa taaccgttgc accagacttc gccgcgacaa aacccgcgcc atcgtagatt
    47881 ttcatcagcg agcctgtagt ggtgatgcgt ccttcaggga aaatcaccac tggtcggccc
    47941 tgttccacca gacgtaccag atgtttaata gccataggtt gcgtcgggtc gagaggaaca
    48001 aagtcgataa atgatttcag ccaacgcata taccactgtt ggcttattga ggtgtaaacg
    48061 gcaaacactg gacgcacagg taaaaacagt ccaagcaaaa tgccatcaat aaaagagacg
    48121 tgattaggcg taattagaac gcgctcgccc ttcagtgcct gggtgtcacc cgtaacgcga
    48181 acgcgataca aaacacggca caaatttcga aaaaagctaa aaagcattcc aactcccttt
    48241 gctctgattc agtaaaagcg aatggaggga gattacacga gataaagaac gcgagcgaca
    48301 gtaaattagg tgcgaaaaaa aacctgcgca tccgcgcagg ttggtgcaag agacagggta
    48361 cgaagagcgt accgaataat ctcaccaatc aatacctctg ggatcaccac tttagcaacc
    48421 tgaagccaaa cgccaccagc ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt
    48481 ttgcaatctc tttttttgtc ttacttattt gcgtttggct cacattccca cgatgaaaac
    48541 acgccacccc ttgaaccaac gggcgttttc cgtaacactg aaacaatgta agcgtttacc
    48601 cactaaggta ttttcatggc gaccataaag gatgtagccc gactggcagg cgtttcagtc
    48661 gccaccgttt cccgcgtcat taataattca cccaaagcca gcgaagcttc ccggctggct
    48721 gtgcatagtg caatggagtc tcttagctat cacccgaacg ccaacgcccg tgcgctggcg
    48781 cagcagacca ctgaaacggt cggtctgatc gttggtgatg tttccgatcc gtttttcggt
    48841 gcaatggtga aagcggtcga acaggtggct tatcacaccg gtaatttctt attgattggc
    48901 aacggttacc acaacgaaca aaaagagcgt caggctattg agcaactgat ccgccatcgc
    48961 tgtgctgcgt tggtcgtcca tgccaaaatg atcccggatg ccgatttagc ctcattaatg
    49021 aaacaaatgc ccggtatggt gctgatcaac cgtatcctgc ctggctttga aaaccgttgt
    49081 attgctcttg acgatcgtta cggtgcctgg ctggcaacgc gtcatttaat tcagcaaggt
    49141 catacccgca ttggttatct gtgctctaac cactctattt ctgacgccga agatcgtctg
    49201 caagggtatt acgatgccct tgctgaaagt ggtattccgg ccaatgatcg gctggtgaca
    49261 tttggcgaac cagacgaaaa cggcggcgaa caggcaatga ccgagctttt gggacgagga
    49321 agaaatttca ctgcggtagc ctgttataac gattcaatgg cggcgggcgc gatgggcgtt
    49381 ctcaatgata atggtattga tgtaccgggt gagatttcgt taattggctt tgatgatgtg
    49441 ctggtgtcac gctatgtgcg tccgcgcctg accaccgtgc gttacccaat cgtaacgatg
    49501 gcgacccagg ctgccgaact ggctttggca ctggcggata atcgccctct cccggaaatt
    49561 actaatgtat ttagtccgac gctggtacgt cgtcattcag tgtcaactcc gtcgctggag
    49621 gcaagtcatc atgcaaccag cgactaaccg cagttaaagc aattccagcg ccagtaattc
    49681 ttcgatggtc tggcgacggc gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg
    49741 taacagcgga cggctattgt agttggatga cattgatgcg ccatatgccc ctgtatcatg
    49801 cagtaccaga taatcacctg ccttcacttc cggcaaggcg cgggtttcaa catttccccc
    49861 ttcctgctgg gtaaagacat cgcccgattc acataacggc ccggcgacga cggtttccac
    49921 cgttggcgcg tgttccagag aacgaccatc agctgccagg gcactgatat ggtggtaact
    49981 accgtacatt gccgggcgca tcagatcgtt gaacccggca tcaaccagca caaagtggcg
    50041 gctacccatt tgtttgacgc tccgcacctg agtaattaat acgccagact gcgctaccag
    50101 gaagcgaccc ggttcaattt ccagtttcac agggtggccc aaatggcgag cgatttgctc
    50161 acgcgcggca ttccacagac cataataatg ttcggtatca accgcctctt caccctgttg
    50221 ataaggaata gaaagcccac cgcccgcaga aatagcctgt aaatcctgac cgaattcgat
    50281 gacctgacgc accatagcac cacacacctg ctccagatgg gcataatcaa cgccagaacc
    50341 aatgtgcatg tgaatgccga ctagctgcag atgatgacgt tgtatcacgt ccagtgcggc
    50401 gggcagatcg gtgtaccaga taccgtgctt gctgttttcg ccaccggtat tggttttttg
    50461 gctatgtccg tgaccaaacc ccggattaac gcgcagccat acccgatgcc ctggcgaaac
    50521 ctggcccagt tggtcgagca tatcaacaga acccgcattc accggaattt gcaattcact
    50581 gacgcgttca agcgtcgcct gatcgataac atctgccgta aaaacaatat catcggggtg
    50641 cgtttgcgga ttgtaacccg ccgccaacgc acgctctatt tcgcctaacg agacggaatc
    50701 cactttcacg ccctgctcac gcattaagcg caaaatatga atattggaac aggctttctg
    50761 tgcaaagcgc accacttcaa actgtttcag cgctgcaatc tgccgacgaa taatttgcgc
    50821 atcgtagacc cacaccgggc agccaaattc agcgggcaaa cgcagcagat tttcggcggt
    50881 gagatcggta tcggtgctga acagtgaatg tggcataaca aactccagat gagtgctttt
    50941 ttatgattac gccacatcat aaaaagaata aaaaatatcg atttatgtcg aatctatgca
    51001 aaaatgatat ggattaccgg attgcgagag agcgctaatg gccgccgtta acttacgtca
    51061 tattgaaatt tttcatgcgg taatgaccgc cggaagcctg actgaggcag cacacctgct
    51121 acacacctca cagccaaccg tcagccgcga actggcgcgc tttgagaagg tgatcgggct
    51181 gaaattgttt gagcgcgtac gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt
    51241 tgaagaagtg caacgatcct ggtacggact ggatcgcatt gtcagcgccg cagaaagtct
    51301 gcgcgagttt cgccagggag aactgtctat tgcctgcctg ccggtctttt cgcaatcttt
    51361 tttaccgcag ctcctgcaac cctttctggc acgttatccc gatgtcagct taaatatcgt
    51421 gccccaggaa tcaccgctac ttgaagagtg gctctcggcc cagcgtcatg atttaggact
    51481 cactgagaca ctccatacgc ctgcgggaac agaacgtacc gaattactct ctttagatga
    51541 agtgtgtgtg ttacctccgg gtcatccgct ggcggtaaaa aaggtattaa cgccggatga
    51601 ttttcagggt gagaattaca tcagcctttc ccgtactgac agctatcgcc agttgctgga
    51661 tcagctattt actgaacatc aggttaaacg acgcatgatc gtagaaaccc acagcgccgc
    51721 gtcagtctgc gcaatggtac gggcgggggt aggtgtttcg gtggttaacc cgctcaccgc
    51781 actagattat gcggcaagcg gtttagtggt gcggcggttc agtattgcgg ttccgttcac
    51841 cgtcagcctg atccgccccc tgcaccgccc gtcatcagcg ctggttcagg cgtttagtgg
    51901 gcatttacaa gcggggttac cgaaactggt cacttctctt gacgctattt tgtcgtcagc
    51961 tacgacagca taaaagcgac agcatcctcg gcatggatcg ccgcggtatc aaacacaggc
    52021 aaaacactgc gctcttctgg caccagtaaa ccaatttctg tgcagccaaa aatgacgccc
    52081 tgtgcgccct gttctgcaag gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg
    52141 gtaaatttcc ccagacacag ttcttcaaaa ataatctgat taattttcgc ccgttcatcc
    52201 gcttcaggaa taagacaatt gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc
    52261 tgttccatgg tgtaacgcgt acccagcagc gccacacgag tcattccggc cccggtaatt
    52321 gcacgtccgg tggcatccgc aatgtgtaag aaaggcagag tgcaacgtga ctcaatggca
    52381 tccgccactt tatgcatcgt attggtacat aacacaatac cttctgcgcc cgcccgctgt
    52441 aagccaagcg ccgcctcagc cagaatgtcc ccggttttat cccattcccc gcgacgctgg
    52501 cactcttcta tttcatgaaa atcgacgcta tgtagcagca tttgcgcaga gtgaagccca
    52561 ccaagccgct gtttaatgcc ttcatttatc agacgatagt aaggaatggt ggattcccag
    52621 ctcattcctc ccagcaaacc aattgttttc attcctctct cctgatgtgt gttaccgcaa
    52681 caaggctgaa taaggaacga gacaaacgcc tcaacggcca ggtgcccaat ctctattaac
    52741 gaaaaaaggg ccggatgtac agcacatccg gcccgtgaaa tcagacgccg atatttctca
    52801 acttctcgcc tgccatcagt ttgcgttcga tatgttccag cgtgacattt ttggtttccg
    52861 gaatgagcca gaaagtaatg cccacaaacg caatgttcaa cgcagtgtag agccagaacg
    52921 taccggcagc gccaatgcta tcaagcagtg tcaggaaggc cgcgccgata atcatattcg
    52981 acacccagtt tgtcgtcgtc gaacaggtaa taccgaaatc gcggcatttc agcggctgaa
    53041 tttcagagca caggatccac accactggcg cggcgctcat cgcataaccg gcaatacaca
    53101 tcatcgtcat gccaacagag agccaggaca agccactgga agccgtaccg ttatcaaact
    53161 gcatcaggca atagcccagc accagagtgc ctaacgccat cacgctgaaa ccaattttca
    53221 gagccggttt acgacctgct ttatctaccg taaagaccgc aatgaaggtc gcgaacataa
    53281 aggtcagtcc cacgaccaga gtcgcaatca tctgttgttc tgtggtcgta aagcccgcca
    53341 ttttgaagat acgcggcgcg tagtacatga tgatgttcat accggtaaac tgctgcatcg
    53401 cctgcaacaa cataccgaga aacacagcac gacggacgtt acggttgatc ttaaacagtg
    53461 cccaaccgcc ctgttttaac ttcaggcttt cacgaatttc gttgagttct tctcgcgctt
    53521 tttccgacgt atcgcgcagc atacgcaaca cttcttccgc ctcaatatga cgcccctttt
    53581 ccgccagcca gcgcgggcta tttggcagga agaccaccag aataatcagc agaactgctg
    53641 gtaaagcaag aaccccaaca ttgcgcgcca gttaccgcta taactgaacg ctgtatcgga
    53701 taaaaacgcc agcacgatgc cgagtgtgac catcaactgg tacatactga tcatcttacc
    53761 gcgaacgtac ttgccatttc agaaagatac agaggagcgg tgtaagacgc gatcccgaca
    53821 gcaatgccca gcaccacacg agcggcgatt aacatctcta cgctggtcgc aaaagcggac
    53881 cctatagaac cgagtacaaa caggatggcc cccgccatca ggctgtattt acgccccagg
    53941 cggaacgaca gccaaccatt aaacagcgca ccaattgctg cgccgagcat catgctacta
    54001 accacccatt cctgcaaacg actggtcagc acaaagtgat cggtaatgaa cggcaacgct
    54061 ccggcgatta cgccgatatc aagaccaaat aacaatcctg cgaccgcagc agctaccgaa
    54121 acaaacatat tcatacgccg cgtatcacgc aaagaacgtg gcgttaaagc agattccgta
    54181 ttgatagtaa ccattttttc ctgccagcag agagtaagac attgtgaaaa aatacgtgaa
    54241 caattcacac aggtgtcagg tcggaaacag cataaatatg gattaaattg ctgcgacatg
    54301 ccgttatgtg atggatattc caattttaaa attaagttga attattgaga ttattattaa
    54361 ccacctaatt ttaaagtaga taaaattcat aaagttcatt aattgataat tgatatggat
    54421 tatttcataa ccatgatatg gattatgatg atctagagat ataaaaaacc ctgccatgcg
    54481 gcagggtcat aaaagtaaga ggaatgaatt aacgcgccag ccaaccgcca tccacagcaa
    54541 tggtataacc atttacataa tctgaagcgc tggaggcaag aaacactacc ggccccatca
    54601 ggtcactcgg cagtccccaa cggccagctg gaatgcgatc gagaatttcc gcgctacgtt
    54661 gttcatctgc ccgcagttgt tgggtattgt tggtcgccat gtaacccgga gctatcgcat
    54721 taacattaat gttgtgttta gcccattcgt ttgccatcaa tcgcgtcaca cccatcactc
    54781 cgctttttga tgcggtataa gaaggcacac ggatcccgcc ctggaaggag agcattgacg
    54841 cgatattgat aatcttgccg ccattgcctt gcgcgataaa gtgtttcgcc gctgcctgag
    54901 acatgaagaa tacgctcttg atattcaggt tcatgacatc gtcccagtct ttttcgctga
    54961 actcgagagc gtcttcgcgg cgaatcaatc cggcgttatt caccaggata tcaatatgac
    55021 caaattcagc taccgcgcga tccaacagtc caggaatgcc atcaatcttt cgcagatcgg
    55081 cggtcaggct taaaaaacga cgccccagcg ccgtgacctg ctcgatggtt tcagtcggtt
    55141 caacgatgtt aatgccaaca atgtcacagc ccgcttgcgc cagccccaac gccatcccct
    55201 ggcccagccc agtatcacaa ccagtgacga ccgcaacttt accttggaga gaaaatgcac
    55261 ttaaaatcat aacaacacct tattcgttta tgcccacaac tagcgcaaat ctttaacggc
    55321 cacatggtcc atatcattaa agacctggtt ttcaccgacc atgccccaga taaaggtata
    55381 agctttggtt ccgacgccgg aatggatcga ccagctcggg gagatcaccg cctgctcgtt
    55441 atgcatcaca atgtgacgcg tttcttgcgg ctgccccatc atgtggaaaa cgcaggcgtc
    55501 atcatccata ttgaaataga aataaacttc catccggcgc tcgtgggtgt gacacggcat
    55561 ggtgttccac aagttacctg gagccagctc cgtcagcccc atactcaatt gacaggtttc
    55621 cagtacatcc gggacaaaat atttgttaat cgtgcgacgg ttactggtga ggttatcgcc
    55681 taacgtgact ggagatactt cgtccggtgt gacttttttg gtgggatacg tcgtatgcgc
    55741 gggtgcgcaa ttgtaataaa acttcgccgg agtggcggta tcaatactgg caaagacaac
    55801 ttcttttgca cctttaccaa catacagggc gtcgcggtga ccgatttcat agcattgacc
    55861 atcgacagta atcgtaccgg caccgccaat attgataaca cctaactcgc gacgttcaag
    55921 gaaatagctt acgccgagtt gtttaccaac ttccccgcca acggaaaccg ttttagttat
    55981 cggcataatg ccgccaacaa taattcggtc aatgtggctg taaaccatgg tgtactcatc
    56041 ggcgacaaat accttttcaa ccaaaaattc attacgcagc ccttgggtat ccagcgtttt
    56101 tgcgtgcgca ccgtggatgc tctgtcttac gtccacatca acctccgata atcagtgaag
    56161 cgtgaaaata gaaaacgata gtgaaacact gtttcgtttt ctctataaag ggatgatatg
    56221 cccatgggct agggttttca attaaaaata aaacaatgtt ttatttttaa tttaaggatt
    56281 aaaaaaaatg cagtgtcgat cacgaatatg tcatgagaat ggaaaaatga gggagcccag
    56341 aaaatgtatg tcgacttgcc agccatcatt attcaacaga ttaatgacgg cagatattta
    56401 tcggtaagga ggaaatgcag agctactcgt cacgttcaat ggtcaatgcc acgccctagc
    56461 ccccgccgat acaaagcgtt gccagccctt tgcgggcatt acgtttcacc atttcatgaa
    56521 ccagagaaac caggattcgg caaccggaag cgcctatcgg gtgaccgagt gcgatcgcgc
    56581 caccattgac attgacccga cgctcatccc actcaagcat cttgccaacc gaaagcgcct
    56641 gtgcagcaaa cgcttcatta gcctcgataa gatcgacatc agccaactgc cagcctacac
    56701 gctccaggca acggcgggtc gcatacaccg gcgcaattcc catcaatgcc ggatctacac
    56761 caacgctggc aaatgcgcgg atgcgggcca gcacgggtaa attcaacgct tgtgctttgg
    56821 cttcgctcat catcattacc gcagctgcgc catcgtttat ggatgatgca ttccccgcgg
    56881 tcacagaacc gagactatca aatgaaggat ttaaacgggc taaaccttct gcactggcgt
    56941 cagtgcgtgg ctgttcatcg gtatcaacaa ccaacgtctg accgttactt tggatcatta
    57001 ccgggacgat ctcatcttta aatcgtccgg cgtcaatcgc cgctcgcgct ttttgttgcg
    57061 agctaagtgc gtaagcatcc tgcaactgac ggctgatgcc atattcgcga gccagatttt
    57121 cggcggtgac accaatatga taatcattga aggcatccca caacccatca tgcaccagac
    57181 tgtcaaccaa ctggctattg ccaagctgtg caccggtgcg gctatcagtc agaacatgtg
    57241 gtgcgcggct catgttttcc tggccaccgg cgatgacaat atcagcctcg ccacactgta
    57301 tcgcctgagt agccagatgc agtgctttaa gaccggaacc gcaaacgtca ttaatcgtga
    57361 ttgcagaaac gctgttagga agaccacctt taatagccga ttgccttgcc ggattctgcc
    57421 ctgcccctgc agtcaacacc tgaccaagaa ttacttcatc caccgcatat gcaggaacgc
    57481 cggtacgttc tattaacgct ttcacgacca gactaccaag ttccacggcg gaatgacccg
    57541 ctaacgcacc acgaaagcag ccgataggtg tccgtaacgc cccgacaatc acaacgtctt
    57601 tcatcacaac ctcgcaccaa ttaacagtgc aatagtaaat gattgttaat tacagttatc
    57661 ttaattgttt aaaaaaagtg atttttatca caaaggaaat atgcctgagc agtagtcaga
    57721 gtcataactg gcacgtaagg tttgcaacca ctaacccacc aatagatggg tagatagggc
    57781 gttaatctcc catacttaac ttggtttatg gtaaattgcc ctccattttg tttaatttgt
    57841 agatgatacg ttcagataat gtctaatatt tggtctaaag aagaaactct gtggagtttc
    57901 gcgctctacg gcacagccgt tggtgcaggc acgctattcc ttcctattca gttaggttcg
    57961 gcaggggctg tggtcctgtt tattactgct ctggtcgcct ggcctttaac atattagcta
    58021 cataaagcct tatgccagtt catcctctca tcaaaaacat cagcaggtga agggataacg
    58081 ggcgcggtaa cacactacta tggcaagaag attggtaatc tgattaccac gctgtacttc
    58141 atcgcctttt ttgtcgtcgt gttgatatat gcagtggcaa ttaccaactc acttacggaa
    58201 cagctggcaa agcatatggt tattgatctt cgcatccgta tgttggtgag tctgggtgtt
    58261 gtattaattc tgaatctcat ttttctgatg ggacgtcatg ccactattcg ggtaatggga
    58321 tttttggtat tcccattgat tgcctatttc ttatttcttt ccatt
//
LOCUS       DAJTYG010000014        57916 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTYG010000014 DAJTYG010000000
VERSION     DAJTYG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.014"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..160)
                     /locus_tag="OO568_000917"
     CDS             complement(<1..160)
                     /locus_tag="OO568_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6640374.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKV"
     gene            315..887
                     /gene="yeiP"
                     /locus_tag="OO568_000918"
     CDS             315..887
                     /gene="yeiP"
                     /locus_tag="OO568_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6640375.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1110..2576
                     /locus_tag="OO568_000919"
     CDS             1110..2576
                     /locus_tag="OO568_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6640376.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2694..3680
                     /gene="yeiR"
                     /locus_tag="OO568_000920"
     CDS             2694..3680
                     /gene="yeiR"
                     /locus_tag="OO568_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6640377.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQLL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3719..4432
                     /gene="lpxT"
                     /locus_tag="OO568_000921"
     CDS             3719..4432
                     /gene="lpxT"
                     /locus_tag="OO568_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6640378.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4844..5410
                     /gene="mepS"
                     /locus_tag="OO568_000922"
     CDS             4844..5410
                     /gene="mepS"
                     /locus_tag="OO568_000922"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6640379.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAMG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5591..7147
                     /locus_tag="OO568_000923"
     CDS             5591..7147
                     /locus_tag="OO568_000923"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6640380.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVARHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHLVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            7229..9043
                     /locus_tag="OO568_000924"
     CDS             7229..9043
                     /locus_tag="OO568_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6640381.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWHFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9044..10138
                     /locus_tag="OO568_000925"
     CDS             9044..10138
                     /locus_tag="OO568_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6640382.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10138..11163
                     /locus_tag="OO568_000926"
     CDS             10138..11163
                     /locus_tag="OO568_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6640383.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11165..12754
                     /gene="yejF"
                     /locus_tag="OO568_000927"
     CDS             11165..12754
                     /gene="yejF"
                     /locus_tag="OO568_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6640384.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12758..13102)
                     /locus_tag="OO568_000928"
     CDS             complement(12758..13102)
                     /locus_tag="OO568_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640385.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13435..14625)
                     /gene="bcr"
                     /locus_tag="OO568_000929"
     CDS             complement(13435..14625)
                     /gene="bcr"
                     /locus_tag="OO568_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR6640386.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAVFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14653..15348)
                     /gene="rsuA"
                     /locus_tag="OO568_000930"
     CDS             complement(14653..15348)
                     /gene="rsuA"
                     /locus_tag="OO568_000930"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6640387.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15498..17258
                     /locus_tag="OO568_000931"
     CDS             15498..17258
                     /locus_tag="OO568_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6640388.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDPPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17383..17667
                     /gene="rplY"
                     /locus_tag="OO568_000932"
     CDS             17383..17667
                     /gene="rplY"
                     /locus_tag="OO568_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6640389.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEINVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17806..18813)
                     /gene="yejK"
                     /locus_tag="OO568_000933"
     CDS             complement(17806..18813)
                     /gene="yejK"
                     /locus_tag="OO568_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6640390.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            18995..19222
                     /locus_tag="OO568_000934"
     CDS             18995..19222
                     /locus_tag="OO568_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6640391.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            19242..21002
                     /gene="pbgA"
                     /locus_tag="OO568_000935"
     CDS             19242..21002
                     /gene="pbgA"
                     /locus_tag="OO568_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6640392.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDTVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21077..21153
                     /locus_tag="OO568_000936"
     tRNA            21077..21153
                     /locus_tag="OO568_000936"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21111..21113,aa:Pro,seq:ggg)
     gene            complement(21256..23847)
                     /gene="yejO"
                     /locus_tag="OO568_000937"
     CDS             complement(21256..23847)
                     /gene="yejO"
                     /locus_tag="OO568_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR6640393.1"
                     /translation="MHQSCSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24167..24814
                     /gene="narP"
                     /locus_tag="OO568_000938"
     CDS             24167..24814
                     /gene="narP"
                     /locus_tag="OO568_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6640394.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGNKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25025..26077)
                     /gene="ccmH"
                     /locus_tag="OO568_000939"
     CDS             complement(25025..26077)
                     /gene="ccmH"
                     /locus_tag="OO568_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6640395.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDTNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26074..26631)
                     /gene="dsbE"
                     /locus_tag="OO568_000940"
     CDS             complement(26074..26631)
                     /gene="dsbE"
                     /locus_tag="OO568_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6640396.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26628..28571)
                     /gene="ccmF"
                     /locus_tag="OO568_000941"
     CDS             complement(26628..28571)
                     /gene="ccmF"
                     /locus_tag="OO568_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6640397.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28568..29047)
                     /gene="ccmE"
                     /locus_tag="OO568_000942"
     CDS             complement(28568..29047)
                     /gene="ccmE"
                     /locus_tag="OO568_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6640398.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29044..29253)
                     /gene="ccmD"
                     /locus_tag="OO568_000943"
     CDS             complement(29044..29253)
                     /gene="ccmD"
                     /locus_tag="OO568_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6640399.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29250..29987)
                     /gene="ccmC"
                     /locus_tag="OO568_000944"
     CDS             complement(29250..29987)
                     /gene="ccmC"
                     /locus_tag="OO568_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6640400.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30029..30691)
                     /gene="ccmB"
                     /locus_tag="OO568_000945"
     CDS             complement(30029..30691)
                     /gene="ccmB"
                     /locus_tag="OO568_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6640401.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30688..31305)
                     /gene="ccmA"
                     /locus_tag="OO568_000946"
     CDS             complement(30688..31305)
                     /gene="ccmA"
                     /locus_tag="OO568_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6640402.1"
                     /translation="MLEAREVLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31324..31926)
                     /gene="napC"
                     /locus_tag="OO568_000947"
     CDS             complement(31324..31926)
                     /gene="napC"
                     /locus_tag="OO568_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6640403.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(31936..32385)
                     /gene="napB"
                     /locus_tag="OO568_000948"
     CDS             complement(31936..32385)
                     /gene="napB"
                     /locus_tag="OO568_000948"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6640404.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32382..33245)
                     /gene="napH"
                     /locus_tag="OO568_000949"
     CDS             complement(32382..33245)
                     /gene="napH"
                     /locus_tag="OO568_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6640405.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33232..33927)
                     /gene="napG"
                     /locus_tag="OO568_000950"
     CDS             complement(33232..33927)
                     /gene="napG"
                     /locus_tag="OO568_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6640406.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(33934..36420)
                     /gene="napA"
                     /locus_tag="OO568_000951"
     CDS             complement(33934..36420)
                     /gene="napA"
                     /locus_tag="OO568_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6640407.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36417..36680)
                     /gene="napD"
                     /locus_tag="OO568_000952"
     CDS             complement(36417..36680)
                     /gene="napD"
                     /locus_tag="OO568_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6640408.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36670..37164)
                     /gene="napF"
                     /locus_tag="OO568_000953"
     CDS             complement(36670..37164)
                     /gene="napF"
                     /locus_tag="OO568_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6640409.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37572..38060
                     /gene="eco"
                     /locus_tag="OO568_000954"
     CDS             37572..38060
                     /gene="eco"
                     /locus_tag="OO568_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6640410.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38210..39241)
                     /locus_tag="OO568_000955"
     CDS             complement(38210..39241)
                     /locus_tag="OO568_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR6640411.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRTKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFELVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39475..39834)
                     /locus_tag="OO568_000956"
                     /pseudo
     CDS             complement(39475..39834)
                     /locus_tag="OO568_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40052..41695)
                     /gene="yojI"
                     /locus_tag="OO568_000957"
     CDS             complement(40052..41695)
                     /gene="yojI"
                     /locus_tag="OO568_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6640412.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPHAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVPLLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41771..42365)
                     /gene="alkB"
                     /locus_tag="OO568_000958"
                     /pseudo
     CDS             complement(41771..42365)
                     /gene="alkB"
                     /locus_tag="OO568_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            complement(42365..43429)
                     /gene="ada"
                     /locus_tag="OO568_000959"
     CDS             complement(42365..43429)
                     /gene="ada"
                     /locus_tag="OO568_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6640413.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43503..44558)
                     /gene="apbE"
                     /locus_tag="OO568_000960"
     CDS             complement(43503..44558)
                     /gene="apbE"
                     /locus_tag="OO568_000960"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6640414.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44670..45791)
                     /gene="ompC"
                     /locus_tag="OO568_000961"
     CDS             complement(44670..45791)
                     /gene="ompC"
                     /locus_tag="OO568_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6640415.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46529..49201
                     /gene="rcsD"
                     /locus_tag="OO568_000962"
     CDS             46529..49201
                     /gene="rcsD"
                     /locus_tag="OO568_000962"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6640416.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPRIEKYISDIDSYVKSLL"
     gene            49218..49868
                     /gene="rcsB"
                     /locus_tag="OO568_000963"
     CDS             49218..49868
                     /gene="rcsB"
                     /locus_tag="OO568_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6640417.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGLLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50068..52917)
                     /gene="rcsC"
                     /locus_tag="OO568_000964"
     CDS             complement(50068..52917)
                     /gene="rcsC"
                     /locus_tag="OO568_000964"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6640418.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53192..53968)
                     /locus_tag="OO568_000965"
     CDS             complement(53192..53968)
                     /locus_tag="OO568_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6640419.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQISGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(53973..55622)
                     /locus_tag="OO568_000966"
     CDS             complement(53973..55622)
                     /locus_tag="OO568_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6640420.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLAFLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVSSMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVGIRTFLQQNANREGDCLTIPGSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55623..57916)
                     /locus_tag="OO568_000967"
                     /pseudo
     CDS             complement(55623..57916)
                     /locus_tag="OO568_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
BASE COUNT        14151 a        15634 c        14239 g        13892 t
ORIGIN      
        1 agactttcgg tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa
       61 tgcagggcgt attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag
      121 gggcagtgca acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta
      181 accaccggat tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata
      241 ccacttgccc gaaaggccca tcgcgagtac tttgtcgcga tatttttgac attttcgact
      301 acaggaattt ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca
      361 acggcaaact gctgctggct aaggatattg atattcagtc gcccactgcc cgcggtgccg
      421 ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt
      481 tcaaaggtga tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct
      541 atgtcgatgg caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca
      601 ccaaagatca gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc
      661 aggtgctgac ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg
      721 aaatcgttga aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg
      781 cgacattgag cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa
      841 ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg
      901 cagaaaaggg atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg
      961 cgcttcctga acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc
     1021 atcgcattaa caatccagac caatttcaat tgctgtcata taactttaca ctgtcattgt
     1081 taatttatcg ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc
     1141 atcatgtaca cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg
     1201 gctttggcgc ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc
     1261 agggcggcga ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc
     1321 agctccgcgc acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg
     1381 tgataattgt cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa
     1441 ttattgagaa attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag
     1501 gctattgtat tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg
     1561 atctacaaac ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac
     1621 gccgccgtga gcgcggcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca
     1681 atggccatgt ggtgaaaaac gcggtgctga gaatggcaga aaaacgttcg ccagaactcg
     1741 ccgggtggat aaaagaacac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg
     1801 ctgcaaccga cgaatcactg gcagaaatca gccagcatct gggggtaaat gatccctgcg
     1861 cgattagctg cgaaccgttt atccagtggg tggttgaaga taacttcatc gctgggcgtc
     1921 ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga
     1981 aactgcgtat gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat
     2041 tcgcctatat cagtgattgc atgcaggatc gcgtatttcg ctatgctgcc agaacattaa
     2101 tgctggatga gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg
     2161 ataagttaat tgcacgtttt gctaatccgt cgctgaaaca taagacctgg caaatcgcga
     2221 tggatggcag ccagaaatta ccgcaacgta tgctggcagg tattcgcata catctggggc
     2281 gtgaaacgga ctggtcgttg ctggcattag gcgttgctgg ctggatgcgt tacgtcagcg
     2341 gcgttgatga tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg
     2401 aacttgttgc gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag
     2461 ttttcggtga tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc
     2521 aacaaatcgt acaattcggc gcacatcagg cgctattaaa caccctcaaa atttaacgat
     2581 ttctgcggtt aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgcct
     2641 tttttagcca ggattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca
     2701 ggaccaacct catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt
     2761 tagcccataa agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg
     2821 gaattgatgg tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct
     2881 gcatgtgctg cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg
     2941 gaaaaccaga ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagctcctcg
     3001 atctgttaac cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc ttgtgcattc
     3061 tcgatccacg cctgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc
     3121 tggctgccgc agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc
     3181 aagcgctaca gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc
     3241 atgggaaagt tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg
     3301 ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc
     3361 cagagcatca acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct
     3421 ggatattcga cgctgatacg gtattcgaca ccattggcat tctggaatgg gcgcgacttg
     3481 caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc
     3541 gtcagggcga tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg
     3601 agctgatttc cagcagcgaa gctgactgga atgccttgca gagcgcgctg ttgaagcttc
     3661 gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat
     3721 gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc ggccccgcgc tgtttctttc
     3781 ctggtatatt cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt
     3841 ctttaatcag aaactggtcg aaagtaaggc ctttttatgg ctggttgcat tgactaacaa
     3901 tcgcgccttc gacggttgtt cactgctggc gatgggtatg ttgatgctga gtttctggct
     3961 gaaagaaaac gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac
     4021 tgcagtggta ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac
     4081 attgactttt accgatatta accgcgtcag cgaactgctc tctgttccca cgaaagatgc
     4141 ctcacgagat agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat
     4201 gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt attatttttg tggtttttgc
     4261 atttcccaga gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac
     4321 cgtgatattg atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac
     4381 cttttttgac aaatcactac caggaaaaaa caaacatttc caaaacaaat aactcacagt
     4441 aattaacatc atcagggtta tttttatagt gaggataatc ctgatgatgc gcaccgtgct
     4501 ttcatctatc gaacgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac
     4561 tcaattgtac tttaattgac caaccccgct tattaacttt ctgtatcact ttttcttata
     4621 aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt
     4681 gcctcggcgg tagattatcc tcacagcata taattttgtg cgttagtcca cagatttggc
     4741 cttaaggaat tgtttcaaca tgcccaggta attagtctcg tgtcgcttgg cattttttta
     4801 taacgatatt tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc
     4861 gattttgaga tatatcttgc gcgggattcc cgcgattgca gtagcggttc tgctttctgc
     4921 atgtagtgca aataacaccg caaagaatat gcatcctgag acacgtgcaa tgggtagtga
     4981 aacatcatca ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt
     5041 aaaatcgcga attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg
     5101 cagcactaaa aaaggtatcg attgttctgg tttcgtacag cgtacattcc gtgagcaatt
     5161 tggcttagaa cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtttcccg
     5221 cagtaatttg cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt
     5281 cggtatttat atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat
     5341 ttccagcatg aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag
     5401 ccgcagctaa taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag
     5461 cactgcttag gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct
     5521 tgatccaggc tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat
     5581 taacggaatc atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat
     5641 tgtagcaggc gtgatgattg cgatactggt tagctgcctt cagtttttag tggccaggca
     5701 taagcacgaa gtcaaatacg acacactgat tatcgacgta caaaagtatc tcgataccta
     5761 ttttgccgac ctgaaatcca ctactgaccg gcttcagccg ctgaccttag atacctgcca
     5821 gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt
     5881 gctggtgaaa gataaaaaaa cattctgttc atctgcgact ggtgagatgg acattccact
     5941 caatgaattg attccggcgc tcgacattaa taaaaacgtc gatatggcga tcttacccgg
     6001 cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa
     6061 aaatagcggt gtgtttgccg ctctgaatct caacctgacg ccttctctct tttatagttc
     6121 acggcaggaa gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt
     6181 ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg ccggtccgtg aaactaaaat
     6241 tgcgggcatt cctctgaccg ttcggcttta tgcggatgac tggacatgga acgatgtgtg
     6301 gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt ggcctgcttt gctattacct
     6361 gatgagcgta cgtatgcgcc ccggcagaga aatcatgacc gccatcaagc gcgaacaatt
     6421 ttacgtggcg tatcaaccgg tggtggatac acaagctttg cgggtaacgg gcctggaagt
     6481 actgctacgc tggcggcatc ctgtagcggg agaaattccc ccggatgcct tcattaactt
     6541 tgccgaatcg caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg
     6601 cgatgccgca gaattagaaa aagtactgcc ggtaggcgtc aaatttggca ttaacattgc
     6661 gccggaccat ctgcacagcg aaagctttaa agcagatatc cagaaactgc tcacttccct
     6721 gcccgcacac catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca
     6781 agaagccaca caactcttcg cctggctgca cttggtcggc gtagaaattg ctattgatga
     6841 cttcggcacc gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa
     6901 aattgaccgt ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga
     6961 cgcggtgctg acgctggcga aacgtctcaa tatgctgaca gttactgaag gggtcgaaac
     7021 gccggaacaa gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat
     7081 tagtcgcccg ttaccgctgg acgattttgt tcgctggctg aagaaaccgt atacgccgca
     7141 gtggtaaggt gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta
     7201 atgatgagta aaaggaaatc cgttgcagat gattgtgcgc atactgctgc tgtttatcgc
     7261 tctgttcacc ttcggtgcgc aggcgcaggc tatcaaggaa agctatacct ttgccgtgct
     7321 gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc
     7381 aaaaggtggg cagataacgt tgtcagccct cggtaccttc gataatttca accgctatgc
     7441 actgcgcggc aacccgggcg cacgcaccga gcagctgtac gacacgttat ttacgacttc
     7501 cgatgacgaa ccaggcagtt attacccgct gattgctgaa agtgcacgct atgctgacga
     7561 ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttctcccat
     7621 tactgcccgc gatgtagagt ttacttttca aaaatttctg accgaaggcg tgccgcaatt
     7681 tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga
     7741 gttagctaaa cccggcaaag aagatatgtt gagtctgttt tcgctgccgg tatttccaga
     7801 aaagtactgg aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg
     7861 tccgtaccac gttacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga
     7921 ttactgggca gcaaacttac cggtaaaccg tggacgctgg catttcgaca ccattcgcta
     7981 cgattattac ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt
     8041 gcgtatggaa aacgacgcta aaaactgggc cacgcgttat accggtaaaa atttcgataa
     8101 aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc
     8161 gtttaatatc caacgtgcgg tattcagcga tcgccgggtg cgggaagcaa tcactctcgc
     8221 ctttgacttt gaatggatga acaaggcgtt gttttacaac gcctggagtc gcacgaacag
     8281 ttattttcaa aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct
     8341 tctggcacca atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc
     8401 ggtatctaaa ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa
     8461 cgaagcgggc tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccgct
     8521 cagctttgaa ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca
     8581 cagcctgcaa cggctgggta tcaatatgga cattcgcaag gtggataact cgcaaatcac
     8641 taaccgcatg cgcaatcgcg actatgacat gatgccgcgc ctatggcggg cgatgccgtg
     8701 gcccagttcc gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc
     8761 ccccggcgtg caaagcccgg ttatcgactc gctgatcaac caaattattg ccgcgcaggg
     8821 aaataaagaa aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta
     8881 ttacatgctg ccaatgtggt acatggcgga agaccgtctc gcctggtggg ataaattctc
     8941 ccaacccgct gtacgccctg tttacagcct aggtatcgat acctggtggt atgacgttaa
     9001 taaagcggca aaactgccgt cagccagaca acagggagag tagatgggcg cttacctgat
     9061 tcgccgtctg ttgctggtga tcccaacgtt atgggctatt atcactatca actttttcat
     9121 cgtgcaaatt gcgcctggcg gtccggtcga tcaggccatc gccgccattg agtttggtaa
     9181 tgccggagta ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg
     9241 tgtcggcaat atcagcgaca gtaattaccg tggcggacgc ggattagatc cagaagtgat
     9301 cgctgagatc actcatcgct acggtttcga taagccgatc cacgaacgtt acttcaaaat
     9361 gctctgggac tacatccgct ttgattttgg tgatagcctg tttcgcagcg cgtcggtgct
     9421 gacgctgatt aaagacagtc tgccggtttc catcaccctc ggattgtgga gtacgctgat
     9481 tatctatctg gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagtcgctt
     9541 tgacgtctgg agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc
     9601 catcctgctg attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg
     9661 cctggtttcc gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg
     9721 gcatatcacg ctaccggtgc tggcgacagt gattggtggc tttgcggcgc tgaccatgct
     9781 gaccaaaaac tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa
     9841 aggggtaagt gagaaaaaca ttctctggaa acatatgttc cgcaacgcca tgctgctggt
     9901 gattgccggt tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga
     9961 ggtgatgttt tcactcaatg gtcttggctt actgggctac gaagcgaccg tctcgcgcga
    10021 ttatcctgta atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat
    10081 tgtcagtgat atcagctata cgctggttga tccgcgtata gattttgagg gacgttaatg
    10141 tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt ttcgtcataa ccgtcgcggc
    10201 tactggtcgt tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc
    10261 gctaacgata aaccgttgct ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa
    10321 aactacagcg aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg
    10381 tggctgaaac aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt
    10441 gctaccagta tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac
    10501 tggctgggaa cggatgcaaa cggcggcgat gtgctggcac gcattctcta tggcacgcgg
    10561 atctcggttc tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg
    10621 ggggcgctac aaggctatta cggcggtaaa gtcgatctct gggggcaacg ctttattgaa
    10681 gtatggtcgg ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct
    10741 aacttctggt ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg
    10801 gtgcgggcgg agtttttacg tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt
    10861 ggcgtcagcg atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc
    10921 ctcacctttt taccgtttat tttatgtagt tcgataacca ccctgacctc gctcgatttc
    10981 ctcggcttcg gtctaccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa
    11041 aataaccttc aggccccgtg gcttgggatc accgccttct tgtcggtggc gatattattg
    11101 tctttgctga tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg
    11161 tagcatgacg caaactctgt tagcgattga aaatttgtcg gtgggttttc gccatcagca
    11221 aaccgtacgt acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc
    11281 gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct
    11341 cccttccccg ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct
    11401 tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca
    11461 ggaaccgatg gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct
    11521 ttcactccac cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta actgccttga
    11581 tcgcgtcggt atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg
    11641 cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat
    11701 tgccgatgaa ccgaccaccg cactggacgt ctctgtccag gcgcagattt tacagctgtt
    11761 gcgcgaactg caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat
    11821 tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa
    11881 taacgccgct acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag
    11941 tgaaccgtca ggcgatccgg taccgttgcc agaacctgcc tcaacgttgc tggatgttga
    12001 acagcttcag gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa
    12061 tgtggtggtg aaaaacatca gttttacgct acgggcgggt gaaacactgg gtttagtggg
    12121 cgagtctggt tccgggaaaa gcacgacggg actggcgctg ctgcgactga ttacctctca
    12181 gggcagcatc gtctttgacg gtcagccact gcaagattta aatcgccgcc agctgttacc
    12241 tactcgtcat cgtattcagg tggtatttca ggatccaaac tcctcgctca acccacgact
    12301 caacgttttg cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc
    12361 acaacgcgaa caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg
    12421 ccaccgttat ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc
    12481 attaattctt aagccctcgc tgatcatact tgatgaaccg acatcatcac tcgacaaaac
    12541 ggtacaggcg caaatattga cgcttttgaa atcattgcaa caaaagcatc aactggccta
    12601 tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact
    12661 gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca caccgcagca
    12721 ggagtatacg cgtcagttac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa
    12781 agggttcggc aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct
    12841 ggcgatttac aaacaggcac ggctcacctt cacactccaa caccgaacat ggaacttcga
    12901 tgtcgcttaa cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc
    12961 taagcaattt aagcgccacc aggctgttgt caccgcacgg tccattttgc ggaatcggtt
    13021 caaccttcga acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt cgatggacga
    13081 ttgagagttg taatgaagtc acttattttc cccggaagta cattcacttc acaatttgtt
    13141 tacatcaatt ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc
    13201 ctcgcctgcg tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt
    13261 gcggggctat atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg
    13321 actttttaca taaattgatt ttacataaaa taaacatata tcggggaaat gatagatttg
    13381 tggttgacat gcatcaacaa aggaagcctt ttaccttcct cgttgtgcaa tagatcaccg
    13441 ttttttcggc cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat
    13501 tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat
    13561 tgcccctatg ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg
    13621 aaactcatca agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa
    13681 caccgcaacg ccaaccacca gcgaccagaa tcccagcccc agcagcgcac tgatgaccat
    13741 ccacgctgcc ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat
    13801 gcggcggacg aagcggctgt taaagatggt catcacgaac agaaaaacaa tgtttagcgc
    13861 aaagtaataa ccaaagtttt ccggcgcgac gtggttaatt tcaatataaa caaacggtcc
    13921 ggcgcttaag aatgagaaca tcccggcaaa gctgaaacca ctggcaagca tgtagctcag
    13981 gacacgttta tggcggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaatgg
    14041 ctgacgacgc tccggtggta aggtttcttt aatcaggaag aaaatcattg ccgaagccag
    14101 aatcgccgct aatgccagga tccagaagat gtaatgccag ctcagccaca ccagcaccca
    14161 gccgccaact atcggtgcca tcagcagtgc aatggtcgtc accagcatga caaacgacat
    14221 catccgcgag aactcttctt tcgggtaaat atcacgcatc aaagcgttga taaccacgct
    14281 ggccgcagcc gcagccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtttg
    14341 tgccaacgca cacgccaccg cggcggcggc aaacaccagc gtcccgccaa gaaccaccgg
    14401 cttacgcccg aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag
    14461 aatataagta ctgagggtca tctgcgtact gcccgccgat acgccaaact gcgctgaaat
    14521 taccggtagc gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc
    14581 aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa
    14641 gtcattgaaa agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc
    14701 gggggctaaa tcagcatcca gcgtaatacc gccaatacgt tcacgatgca gctcaaccac
    14761 gtggttaccc acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt
    14821 cagacgaacc tgcgttgggg taatcacttc aagcaccgcc ggcttagtga gatctttttc
    14881 gttatgcagc tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta caggtgattc
    14941 cagtgtcacc agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca
    15001 ctgaccatca tcagtcatca gcaccagacc ggtggtatcg atatccagac gtcctgcggc
    15061 gtgcagtttc cacgctaccg gttcatccag aaaatagagc accgtcgggt gatccgggtc
    15121 gtccgtggag caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccgtgttg
    15181 ctgcgccagc gggttgccat cgtaagcgac atcatgttca gggagcagtt tgaacgctgc
    15241 attacgaacg atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat
    15301 agcacggcta acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt
    15361 gcctgtaaaa aacggaggtc gggtaatgac ccgaaaatct gaactgttgt ctgcccagta
    15421 tagcggtcta ataacttccc tcaagggaaa aagaaccgtg gcatactatt agcagaataa
    15481 tctacctacg cgaaaccatg atttttacac tccgcccata tcagcaagaa gccgtggatg
    15541 ccacgctcaa ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg cccaccggcg
    15601 caggtaaaag cctggtgata gcggaactgg cacggctggc gcgtggtcgc gtactggtgc
    15661 tggcacacgt taaagaactg gtggcgcaaa accatgcaaa gtatcaggcg ctggggctgg
    15721 aagccgatat ttttgccgcc gggctaaagc gcaaagagag ccacggtaaa gtggtatttg
    15781 gcagcgttca gtcggtcgcc cgtaatcttg atgcctttca gggtgaattt tcgctgttga
    15841 ttgtcgatga atgccaccgt attggtgacg atgaagagag ccagtatcag caaatcctca
    15901 ctcacctgac taaagtgaat ccccacttac gcctgctggg gctgactgcc acgccttttc
    15961 gattgggcaa aggctggatc taccagttcc attatcacgg catggtacgc ggcgatgaga
    16021 aagccctttt ccgtgactgc atttatgagc tgccgctgcg ttatatgatt aaacacggct
    16081 atctgacgcc gccagaacga ctggatatgc cagtagtgca atacgatttc agccgcttgc
    16141 aggcacagag taacgggctg ttcagcgaag ctgatctcaa ccgtgagctg aaaaaacagc
    16201 aacgcattac gccacatatc atcagccaga tcatggagtt cgccgaaaag cgcaaagggg
    16261 taatgatttt cgccgccacc gttgaacacg caaaagagat tgtgggatta ctgcctgccg
    16321 aagatgctgc actgattact ggcgaccccc ccggcgctga gcgcgatgtg ttaattgaag
    16381 attttaaagc ccagcgtttt cgctatctgg tcaacgtcgc agtactgacc accggatttg
    16441 acgccccgca cgtcgatctt atcgccattc tgcgccctac cgaatcagtg agtctttacc
    16501 aacaaattgt cgggcgcggt ctgcgtctcg ctccgggcaa gactgattgt ttaattcttg
    16561 attatgcggg taatcctcac gatctctacg ctccggaagt tggtacaccg aaaggcaaaa
    16621 gtgacaacgt tccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg
    16681 ggaaaacgac cgccgacggg acattgattg aacactttgg tcgccgctgt cagggatggt
    16741 ttgaagatga cgacggtcat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc
    16801 cgcaatgtaa cgcagaaaat gatattgccg ctcgccgctg ccgcgaatgt gacacggtac
    16861 tggttgatcc ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg ctggtattac
    16921 gctgtagcgg catgtctttg cagcatgggc acgacgagaa aggcgaatgg ttgaaaatca
    16981 cctattacga tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa acgcccgccc
    17041 agcgtaccgc cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatccctc
    17101 tgcgctggat caccgccgcc gatatcctcg cccagcaagc cttactgcga catccggatt
    17161 ttgtcgtcgc ccgcatgaaa ggtcagtact ggcaagtgcg tgaaaaagtg ttcgattacg
    17221 aaggtcgttt tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata
    17281 aggcgattga gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc acttacctgt
    17341 tgctgggtcg cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca
    17401 gaagtacgta aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc
    17461 ccggcaatca tctacggtgg caaagaagca ccgctggcta tcgagctgga tcacgacaaa
    17521 gtcatgaaca tgcaagctaa agctgaattc tacagcgaag ttctgaccat cgttgttgac
    17581 ggtaaagaaa tcaacgttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg
    17641 cagcacatcg acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg
    17701 cggggttttt tgtatctgca gattatgcct gattacggta ttgctatttt ttgcaggcca
    17761 gataaggcgt tcacgccgca tctggcattc agaacacaaa accgattaat tgccgccaga
    17821 cgtccggcgc tgcaattggt cgcgcaaatt cggcggcgtg cctttaatgg tcaaggtatc
    17881 ggttgctgga tcccagaaga tgcgttcacc taacagcatc gcatcaaagt taatagtcaa
    17941 gccaccgccg ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt ctgccgggaa
    18001 gctctcttcc agttcgtagc ctttttcagc cgcaaactcc gtgaagctca cttcgcttac
    18061 gcctgccagc tctttcgaca gcgattccag ttcgatctct tcgcctgcct gcagctgctc
    18121 attacagtag ctataaacct gctggcgcac gttctgtcgc tctgctttat ccagttgtgc
    18181 ttcggcagtg aaatcatcaa ccgcttgcaa cagcccacgg ttttgcgctt tcgcgttcaa
    18241 tccttcgctg gccccgagga aatccataaa gaaatccgcc actttgcgcc ctactcgccc
    18301 tttcaggaaa gtgagatagc gggtggactc tggattggtt tcccattcgg ttaaatcgat
    18361 acgcgcaaca atatccgcat gattgatatc aagataatgg gttgggttga tgtcgagatt
    18421 ttcgttaacg cgcatactgc tcaggttgct cagaactgcc accagcagat actccaccgc
    18481 cagataacgg tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa
    18541 ttcgtcacgc aggcgaccgg ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg
    18601 acgctgtaag cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt
    18661 atttttggcg ctatagaccc gatgcagttc tgccaccatc tcaacgacgg tttctgtcgg
    18721 ttccagcaat gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg
    18781 gtgcagagca atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg
    18841 tattcaacca ccgccggtta agcgacgcaa taaaagatgg cggtcatttt acagaaggca
    18901 gcgtaacgag aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata
    18961 tgttgccctt tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtaa
    19021 tgaacaggtt gaacaattgc tcgcagagct gctcaacgta ctggaaaaac ataaggctcc
    19081 gaccgacctt tccctgatgg tgttaggcaa catggtcact aaccttatca acaccagcat
    19141 tgccccggcc caacgccagg caattgccaa ctcttttgcc cgcgccttac agtcctctat
    19201 caacgaagac aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc
    19261 gctaccgtga aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata
    19321 ttctgctttc gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgacaacgc
    19381 ttgctggtcg catttattcc tacgtaagca ttatcggcca tttcagcttc ctggtgttcg
    19441 ccacctactt gctgatcctc ttcccgctga cctttatcgt cggctcccag aggctgatga
    19501 ggtttttgtc cgtcattctg gcgacggcgg gaatgacgct attactgatc gatagcgaag
    19561 tctttactcg tttccatctc catcttaatc ccatcgtctg gcagttggtt atcaacccag
    19621 acgaaaatga gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat
    19681 tgcttgaact ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc
    19741 gacgcttcgc gcgcccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg
    19801 tgtatatctg ggccgatgcc aacttctatc gcccgatcac catgcagcgc gctaacctgc
    19861 cgctttcgta cccgatgacg gcgcgacgtt tccttgagaa gcatggcctg cttgatgcgc
    19921 aggagtatca acgccgtctt attgagcaag gtaatccaga caccgtttcc gtacagtatc
    19981 cgttaagcga actgcgctat cgcgatatgg gcaccgggca gaatgtgctg ttgattactg
    20041 tcgatggcct gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg
    20101 agcaaaatat ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct
    20161 ttggcctgtt ctatggcatc tcgccgagct atatggacgg cattctgtcg acccgtacgc
    20221 ctgcggcatt aattactgcg cttaatcagc aaggctatca gctggggtta ttctcatcag
    20281 atggctttac cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagcg
    20341 tacgcaccca atctgacgat cagaccgcca cgcagtggat caactggctg ggccgctacg
    20401 cacaagaaga taaccgctgg ttctcgtggg tttctttcaa tggcactaac attgacgaca
    20461 gcaatcagca ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga
    20521 tcaaacgcgt gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gtggtgatta
    20581 tcactgccgg tcggggtatt ccactgagcg aagaggaaga aacctttgac tggtcccacg
    20641 gtcatctgca ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg
    20701 ctctgactga tcataccgat ctgatgacga cgctgatgca acgcctgctg catgtcagca
    20761 cacctgccag cgaatattcg caaggtcagg atttgttcaa ccctcaacgc cgtcattact
    20821 gggttactgc tgcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc
    20881 tgaacaataa cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa
    20941 aaccacagtt aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt atcgctaact
    21001 gattaattat aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt
    21061 attagcagcc acgagtcggc acgtagcgca gcttggtagc gcaccgtcat ggggtgtcgg
    21121 gggtcggagg ttcaaatcct ctcgtgccga ccaaaaatcc caagaaaaaa ccaaccctta
    21181 cggttggttt ttttatatct gcaattaatt cgataaacag accgtgacac atcacagcct
    21241 gtttattttc tgttatcaga acgtccagac cacacccgcc tgagtattcc acggcgattc
    21301 tacccctgcg ccattgccat agctgactga caaatgaccg cttaacgtcg gggtaaacga
    21361 tgaccttatc ccagcctgat aaacgccact ggttccagcc acatcattat taaatttgcc
    21421 atcgtcatta actttcacct ggttagaatc ggcgtattcc tgacgcacgg cggctttcag
    21481 ccagggttcc agcgtcgtac cgttttgcag gtccatgtga tagcttaccg ccgttcccgc
    21541 ttcagcgcgt aatatccggg tatttcccac atccgcgcgc atgccgtttg ataacgtgta
    21601 gtcctgacca tctgtggtaa agccggtaaa ggccagatag ggtctaacac tccacaatcc
    21661 gtcaacccaa cggaacccgc tctcaacatg agcacccgcg ccgttactat tgtaatcgcc
    21721 aaacgctgtt gccccattac tcatcttgcc atggatggtg ttggcaaaac ggtcaacttt
    21781 caccacccca tcaacatagg caccgttctg atgctcccaa ccggcataag cccccagggt
    21841 atagctatcg atattacctt ttccgccgcg atcaaaacca atatcagaat gagagtaacc
    21901 aaagaacaag ccgcgaattg tactgctttc ttcacgggag aaacggctat cgataccgag
    21961 cgtcaggccc gtcaatgttt gctcaaaacc agctcccgca tcagtggtca cgttgttgcg
    22021 ggtgttaatt gccgaactcc acatcaccgt atcgtaacta acgcctttta cgctaccaag
    22081 acgctcacgc acggtgtcca gttctgcatc aaataccagc ggttgtgcgg ctgccatgtt
    22141 cagcacatca gtggttgaag gggtaatttg cgcgcgattc tctgcgaggc tccagctatg
    22201 gttgccatta tccagcaagg tatattcata cgtaccgata tcaacaacgc ctccggcatt
    22261 gcccaacgta aatgcagcat caccgccacc cgttgttacc agtgtaaggc tatctcctgc
    22321 tgccgggctg gcaccggtgt ccgtcacgaa tattttgaaa tcacctgttg cctgaccggt
    22381 gacgttgagc tgatcgctct gatgattggc catatcggta cgcatataaa aattgccgtt
    22441 tcccgacagg gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga
    22501 acgagtcact ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa
    22561 gttgaggcga gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt
    22621 ttcgagcgaa acgtccgctt tgctggtatc cacggcacca tgcgttctaa aagaagcgcc
    22681 ctcagaaacc actaccttac ccaccaacat agcgggtgca taatttattt gcggcttcat
    22741 gacctcaaga atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac
    22801 attagcgcgg ccattgttga ttaccatatt ttcagagatg gcttttgagg aatacgtgat
    22861 acttccattc tggtctgatc gaccgagatc atataccgca tttgcctgca ctttcgtacc
    22921 agtatccttt cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg
    22981 cgtgtcctcc ataacaatga gcccggaacc atcatccagt tcataatttt ttgacacacc
    23041 atcctttata ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga
    23101 gacaattaat ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac
    23161 ctccagtaag ccgccactat ccacagtagt attataagcg aagtcatttt cttcaactcg
    23221 caaacttccg ccgttttcca acaacatatt attagcaata ccattcttga tatcgaactg
    23281 accgagacgg tttgttccga atacctccat ggcccgcgtg gttgttttaa tagcaccgcc
    23341 tgctttctga tctaccgcaa gcgcaaatcc cccctcttta acctccagta gtcctccctt
    23401 ttcgatagcg gcaaaaacgg tccccccatc tttttcaacg attaagcgcc cgcctttaat
    23461 cgtggtatca ttcgctgggc cataaacctg tagcacacca ttattgaggg tagtctggtt
    23521 tgccgcgcca cccatttgaa caatttgata accatcattt aatactgagt attctgcggt
    23581 accattcatt tcaatgtatt gataccctcc gttaatttca gtattactac ttacaccaaa
    23641 ttcttttata tgctgttctc cgccaggatt gattttcgta ttattggcgg tcccataaac
    23701 cagctggata tctttttcca ggataacacc atcaactgtc tcaccatact caactgttga
    23761 tgccaatgct atgggtgaat ataacgctgt agccaccaga acagatattg cggaacgaca
    23821 aagagaaaca gaacaagatt gatgcattga gctttcatcc tatgaaatta attgcagtta
    23881 aaagcattgg gtacagaaaa tacccatagc tccatacccg gagtcagttt ttaaaaactg
    23941 tttaaagaaa tgcacaagta ttgtgattga ttttttagtt gtttttcttg atgagaagct
    24001 gatgcaaaat tccgtcttta taatgaaaat gatgccaaag cgaacgacaa ggttgtagtt
    24061 ttcactacat gtccatacat aaaatggggt aacattcacg cgcctggtag cgttaccaac
    24121 gctacgctca aacataatga ttctaataaa acctcaggag actactatgc ctgaagcaac
    24181 gccttttcag gtgatgattg tggatgatca tccacttatg cgacgcggtg ttcgtcagtt
    24241 actggagctt gatcctggct ttgaagtggt cgccgaagcg ggcgacggcg cgagcgcgat
    24301 cgatctggcg aatagactgg atatcgacgt gatcttgctg gatctcaata tgaaaggtat
    24361 gagtggcctg gataccctca atgccttgcg cagggatggc gttaccgcgc aaattattat
    24421 cctgaccgta tccgatgcct ccagcgatgt ctttgcgctg atagacgcag gcgcagacgg
    24481 ttatctgttg aaagacagcg acccggaagt attgctggaa gcgattcgta ccggagcgaa
    24541 aggcaacaaa gtctttagcg aacgcgtcaa tcagtactta cgtgaacgtg aaatgtttgg
    24601 cgcggaagaa gatcccttca gcgtgctgac ggagcgcgag ctggatgttc tgcacgagct
    24661 ggcacagggg ctgtcaaata aacagattgc atcggtgttg aatatttccg agcagacagt
    24721 aaaagtacat attcgcaatc tgctgcgtaa actcaatgtc cgctcacgcg tggcggccac
    24781 cattctgttc ctgcaacaac gcggggcaca ataaaaatag cccgatggat ttatcatcgg
    24841 gctgagattt atgacaaacg caaaactgcc tgatgcgcta cgcttatcag gcctacgtgg
    24901 atcgatcaat ttattgaatt tacacaattt tgtaggccgg ataaggcgtt cacgccgcat
    24961 ccggcataaa ctaagcgcac tttgtcatca gtctagcccg atggcatcac catcgggcct
    25021 ctttttattt actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcaa
    25081 tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa catcatctcc cacgcggcaa
    25141 cggcttcgcc aaatcgctgc tgctcaaagg cattaaacgc atacatgctt agcacacgga
    25201 tattgctatg gtccgttctc accagctgac gtagcagttc accgccgagg cggttgtcgt
    25261 tggtatcaga tgaacgagtc aacgcttcag cgtatcccag tgcggcatcg ctgttcttcg
    25321 gatcgaggcg atacgcagta gcgtatgcat cggtggcgat actggcgtta cccagcgcca
    25381 tgccaacgcg gcccaacata atccagcctt ctatatctcc cggatttttt tgcagttgag
    25441 tacgcatccc cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg gctttcgggt
    25501 ccagcgccct gtccagcaac gccggagcct gtgccgtggc ctgctgccag attttcacct
    25561 gctgataatt gccagtctgg tagtagctga cgccagccac aattaacgcc accacaatac
    25621 ccggcagata aacaacatat ccggcacgct taccttccgg cacgctttgt tcaggaaacg
    25681 cttccggcac cacgcgtacc cgacgccgcg agcgggcgta tatgacccaa ccgccaatgc
    25741 caatagccac tactggcagc acccacagca gcacggtcag cggcgttaac ggcggatcgt
    25801 aagtgacgaa gttgccgtaa cgcgccacca tataatcgac aatctctttt ttacttttac
    25861 cttcctgcat cagttcatac actttctgac gcaggtcggt ggcaatcatc gagttggaat
    25921 cggcaatgct gttgttctgg catttcgggc agcgcagttc ttcagtgagc tgacggaact
    25981 gttgttcctg tgcttcatct ttaaactgca acacgtcgat ggtcgccagc gctgagccgg
    26041 agatcatcag catcagcacg cccaataaaa acctcattgt gcggcctcct tactgtattt
    26101 ctcccacagc ggcttgatct cttcttccca gacgcgagga ttgagatcgc ccgcatggcg
    26161 atagcgaatg ataccgttgc cgtcaataag gaaggtttct ggcgcgccat agacaccgag
    26221 atccagccct aacatgccgt cgccatcaaa caggcttagc gcgtaaggat tgcccagctc
    26281 tttcagccag ctgattgcct tctggcgatc gtctttatag ttcatgccga ccacgcgaat
    26341 gccctgcgct gaaagctgat tcagatattg atgttccgca cggcaggtcg gacaccaagt
    26401 cgcccagacg ttaagcagta ctggtttgcc ctgagtcagc acatccgcct gataaaactg
    26461 ccccggattg tccagtgatt cgagacgaaa cttcggcaca ggcttgccaa tgagcgccga
    26521 ttccagattg gtcggatcat ccccttcggc attacgcgcc agctgccaca gcagcgccgc
    26581 ggcaatcgcc aggaagataa tcaacggaat taacaatact ttgcgcttca tacggcctcc
    26641 ggcgcagttt tttgcggact cacgcgctta cgatagcgag gatcaaacag acacagcagt
    26701 ccgcccaacg ccatcatcag cccgcccgcc caaatccagc gaacaaatgg tttgtagtaa
    26761 agacgcacgg cccacgcgcc gttttccagc tcttcaccga gcgcggcgta caggtcacgc
    26821 gtgatgccgc cgtcaattgc cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt
    26881 ttttccgcat acagcaccgt ttccggttta ccctctcgcg ttacgccaat agtcgccaca
    26941 ccgccgcgcc agttcgggcc agtcacctct ttgacatcac ggaaggtgaa gcgatattca
    27001 tgaatatcga cgctatcgcc ggacttcatg cgcacatcac gctcaacgct ataattctgg
    27061 ctaaaggcaa tgccaacaat tgtcactgcc agccccaggt gagccgccac catcccccaa
    27121 taactgaagg tggttttcgt gccgcgtgaa atacgcagcg cagcttccgc agttgccagc
    27181 accgcaatcc agcaggccat tgccaggccg agcaccgtca tcgccacaac tttgctttcg
    27241 aacagccacg gcaacagcag agacagcacc agcgtagaga tgaaggcgat aatcaataaa
    27301 ttgcggattt tacgtgggcg atcgcgcccc cagcgcacca gaggaccgac accaagcagt
    27361 agcgcaaacg gcaccatcag ccaggtaaac atggtgttga agaacggttc gccaatcgaa
    27421 atactgccca gtccaagttg cttgtgcacc agcggcagca gcgtccccag caacaccacc
    27481 agcatcgcgg cgaccagcaa aacgttgttc gctaacagca aagattcccg cgaccacagc
    27541 gcattgttta cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag cagcgaaccg
    27601 ccaatcacca gcaccataaa ggcgaggata aacataccgc gcgccggatc agacgcaaac
    27661 gcgtgtaccg ataccagcac gccggaacgc acgaggaaag tccccaacag acacaacgag
    27721 aaggcactga ttgccagcag taatgtccac gctttgaagc tggcgcgttg ttcagtgacc
    27781 gccagtgagt gcatcagtgc agtccccacc agccacggca taaacgaggc gttttctacc
    27841 ggatcccaga accaccagcc accccagccg agttcgtaat aggcccatgc ggaaccgagc
    27901 acaatgccga gcgtcaggaa aatccacgcc gccagcgtcc acgggcgagt aaaacgcgca
    27961 taagtgctgt ccagacgccc gctcagcaaa gaagcaatgg caaaagcaaa cgccaccgag
    28021 aaacccacgt accccatata aagcagaggc ggatggaaga tcagccccgg atcctgtaac
    28081 agcgggttaa gatcgcgccc ttcaatcggg aagttcggca gcgtgcgtga gaacgggtta
    28141 gaggtaaaga gaatgaacag caaaaagccg acgctgacca tccccattat cgccagtaca
    28201 cgggccacaa tatccagcgg aatacgctga ctaaaaatcg ccaccgcaaa ggtccagccg
    28261 ctcatcagca gcacccacag cagaagcgaa ccttcatgcg cgccccaggt agccgccacg
    28321 cgataccaca ccggaagctg ggtattggag ttgctggcaa cataggtgac ggtaaagtcg
    28381 ttgaccacga aagcattgac cagcaccaga aatgcgccag ccacagacat aaacagcagc
    28441 caggcaaaca agcgggaaga cgccatcatg cgcgcatctc cgcgcgccac gccccatagc
    28501 ggatacacgg acagcagcag cgcaatccct aacgccaagc acagcagccc gtttccaatt
    28561 tccggcatca tgatgctggg tccttataaa cactcgccgg gcgacggtgg ttagcttcca
    28621 tcgctttctc aacttctggc ggcgtgtagt tttcgtcgtg tttcgccagc acttctttcg
    28681 cgaggatatg attgcctttt tccagctcgc cctgcaccac aacgccctgc ccttcacgga
    28741 acagatccgg caaaatgcct tcgtaagaga catccactga gccttcagca tcgtaaatgg
    28801 tgaaggtcac tttcagcgaa ttgggatcgc gctgcacgct acccggcatc accatcccgc
    28861 caacgcgcag acgctgaccg acttccggca tctgctgagt ttcacgcttg ccgtagagaa
    28921 tttcccccgg cgtataaaag agatcgatat tcgagcgcag cgcatatagc accagaccga
    28981 tagtcagcgc cagccctgcc aacacggcac aggcaatcca caagcggttt ttacggcgaa
    29041 tattcatgca gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg cccgctgttg
    29101 cgccacacca cgcagaattg cgcgatgttg catcaccgag tgcacgacca aaaccaccag
    29161 cggaataacg gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc ccattgcgaa
    29221 aaattcattc caggaagcaa atgcaggggt catttacggc ctcttttcag tatcagttca
    29281 ctcacccacg gacggcgttt ttccatcagc aaaatcaaat tacgcatccg catcagcgtc
    29341 agcgtggcag acaggagcag gaagccaaaa atcgaccagc gcagcggcga acgcatcgcc
    29401 ggatcgatac tttgctgcat ccgcgttgat ccctgatgca gggtgttcca ccactccacg
    29461 gagtaatgaa taatcggcag attcaccacg ccaatcagca ccaggatacc tgccgcacgg
    29521 cccgccagac ggcggtcgtc gaaggcgtgc cacagggcaa tcacacccac atacaaaaac
    29581 agcagcacca gttcagaagt cagacgtgca tcccataccc accaggtgcc ccacatcggt
    29641 tttccccatg cagagccggt aaccagggca ataaaggtaa acacggcacc aatgggggcc
    29701 atcgccgcca ccgccaggtt ggccattttc atctgccaga caaggccaat aaacgccgcc
    29761 accgccattg atgcataaat gcccatcgac cagatcgccg caggcacatg caggtagata
    29821 atgcggtagc tatttccctg ctgataatca gccggagcaa agccgaatcc ccagatccag
    29881 ccgacggtaa gcacgaccac actggcaatt gccagccacg gtataaacca gccacagatt
    29941 tgatacagcc gtggtgggat cgccagttga tgcagtgttt tccacatagt ttcgatacca
    30001 gactcgaaca aaaatcagta atccagcgtt attgaatgct gattcgtaac gctgccgccg
    30061 tcgcaaaagg acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc
    30121 catcaacggg caaatgcata gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg
    30181 ggatagtcag cggtaacacc agtatgctga gcagcacacc accgcgctta agtcccactg
    30241 tcagcgccac gcccggtgca ccgagaaagc caagcgtagg cgttcccagc agcagcgtca
    30301 gcgccatcac ttgccagcca taaacatcca ttcccagtag cattgctacc agtggcgaaa
    30361 ggatgagtaa cggcagaccg gttaccatcc agtgcgccat caccttcgcc agcacaacgg
    30421 cgggcaaggg taacggcaac aacatcaatt gttcaagact gccgtcctgc aaatcgtcac
    30481 ggaacagtcg ttccagcgcc agcaaggatg aaagcagcgc agcaacccag ataatgcccg
    30541 gtgcaatacg cgccagcagt tgcggctccg gaccgatact gagcggaaaa agggtaatta
    30601 caatcaggaa gaaccacagc gggttggcga tttcggcgct atggcgaaac gctacgcgca
    30661 gttcaagacg gaaaatacgc cagaacatca tgcggccctc gtttgcgtca gtgaaatgcg
    30721 gcgaatttta ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca caatcccccc
    30781 ctgctccgta tgctgcgcca tacgctgggt cagacgatcg acgccgttaa cgtcaatcgc
    30841 ggtaaaaggc tcgtcgagga tccataatgt ggcacgggtc aaccacagac gcgctaaagc
    30901 gacgcggcgt tgttgcccgg cagaaagttg atttaccgga atatcttcga atccggcaag
    30961 cccggcctgc gccaaggctt ccagacattg tgcggcatcg ccatcgcgat gataaaagtg
    31021 cagattttct aacgccgtca gccgggtttt gatccccggc tgatggccta tccataacag
    31081 gttttgatgg tagctgtcgc gtacctgatg caagggctgc ccttgccaga gaacctcgcc
    31141 agcgtcaggg cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt
    31201 gctaccggtg atttgtaccc actctcctgc gttcagcgta aatgacaagc cactaaataa
    31261 ggttcgttca tcccgctcac aaagtacctc tctggcttca agcataccca cgcaataacc
    31321 ctgttaaaaa cctggctcga cttcacgcat atcgggcagc ttgtgcgcga tccctttatg
    31381 gcaatcaata caggtttgcc catctttcac cgcctggtca tgcatcttcg cggcaaccga
    31441 tttctgggcg gttgtatcca tatactcgaa gttgtgacag ttacggcact cctgcgagtt
    31501 attgtccttc atgcgccgcc actcattctg tgccatcgtc agacgatgag cttcaaattt
    31561 ctgcggcgtg tcaataacgc caaaaatttt accatacagc tctttacttg ctttgagctt
    31621 gcgtatcatc ttcggcacaa actcgtgcgg aacgtgacaa tccggacagg tcgcacggac
    31681 gccgctacgg ttgttgtagt gcacggaatc catgtattcc tgatacaccg tgttgcgcat
    31741 ttcgtggcag ctaatgcaga actcttcggt attggctttt tccatcccgg tgttaaagcc
    31801 cccccagaag acgatgccgc caacaaaacc gatcaacagc agcgtcccca gcgccagatg
    31861 gctgggggta cgccaccatt tccacaggcg cttaatcaga ccaggcttac ggtcagaatt
    31921 tcccataata acctcttatt tcccgtaacc ttttgatggg gtaaaggtat tccccacgat
    31981 tggcgcggta tcggcctgcg gtacgtgaca ttgcagacag aaataacgac gcggagccac
    32041 ttccgcgccc actttgccgt cgctgtccat aaagtgagta ggactgatac gcggcgcgcc
    32101 agtggtgcga tagctttcga caccgtggca ctgcaagcag cgattggtat tggtcgttac
    32161 ctgataacct tcaacgctat gcgggatcat tggcggctga ttcacatagt tcagcggcat
    32221 ccgatcctgc tcttttggca tccgaatggc cccttcctgc gtcccggata cttccggcga
    32281 ctggctaaaa tcgacgccat tagccgccca aaccgcgccg cttaccacca gggccagcat
    32341 cgccgtccat tgacacagcg ctttcttcag gtcatggctt ttcatgattt cgctcccgaa
    32401 ctccatcgtg tagttattgt aaatacatcc tcagaacaga catccacgca gcgaccgcac
    32461 gtcatgcaat cgcggctggt gacctgcacc gggctttgtt catccagcac cggggcacgt
    32521 agcacatgcg gttccgggca aacatgaaaa caatccatac agcggttaca tttctgccga
    32581 tcgctggccg caacggtaat cacacctttg ctacccagca cgccatacag cgcgccaacc
    32641 ggacaaatgt gcccgcacca gccgtgttca acgaccagta gatcaaataa aaacagcgcg
    32701 agaataagca gcgcgccgct gccgaagccc attaccaggc tacgccccat cagagaaacg
    32761 gggttgatcc attcccaaat gagcgtgcca gttaaggctg atcccaccag aatgaccacc
    32821 aacagcacgt agcgaatgtg gcgagggatc gtcgcagact gattgaggtc aaagcgcctg
    32881 cgtaaccagt tcgctaagtc ggtaatcggg ttcagcgggc agacccagct gcaaaataat
    32941 cgctttcccg ccaaggcgta gagcacggtg ataatcaccg caccggtcaa cgccaccgtg
    33001 gcaggcagat gaccactggc aagactttgc agcgtcatca gcggatcggt cagcggaacg
    33061 gtgtcgaata acaggctact gctgtagttg ccgtgcaaga tccacacacc aaaccatgga
    33121 ccgctcaaaa acatccccag cacgaagaac tgacaaaggc gacgtaacac cagccaacgg
    33181 tgactgcgcc accagccttt tttctccagc gcctcgcgcc cggcgtcacg tttacgattt
    33241 gccattgttc ccctccagcc agccgaagcg gtaatggtga cctaactccc ctttcgccag
    33301 tgacagcggt aacaccttga ttgccggttg ttccagcacg catacttttt cgcacttacc
    33361 gcaaccggta caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct taccggtacg
    33421 cgtgttgcgc tccagctcca aggtgatggc ctcatcaatt ttcgggcatt cgcgataaca
    33481 aacatcgcag cgcagcccct gaaagttgag acagttttcc tggtccacca gtaccgccag
    33541 ccccatccgc gcgtcgtcga tcgattcaat ctcacgatcc agcgcaccgc ttgggcacac
    33601 tttggcgcac ggaatgtcct cacacatttc gcaaggaata tcccgtgcga caaaatatgg
    33661 cgtgcccgcc gacagaccag aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc
    33721 ctgaacacac tgaccgcaac gcacacaggc actggcaaag gcgttctcgt ttatggctcc
    33781 gggcgggcgc aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac
    33841 acccacggca gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc
    33901 attttgaggt ttcgctgacc gggacataat ggcttacacc ttctccagtt tgaccgcgca
    33961 cttcttgaag tccgtctctt tcgagagcgg atcggtcgca tccagcgtca gtttgttaac
    34021 cagctgtgcg gcgtcgaaga acggcatgta caccaggccc tgtggcggac ggttacgacc
    34081 gcgcgtttca acaatcgaga tcacttcgcc acggcgagaa accactttca ctttgtcgcc
    34141 acggcgcaga tcgcgcgctt tcgcatccag cgggtgaata aacaagaccg cttccgggaa
    34201 ggcgcggtgc agttccggca cacggcgagt catactgccg gtgtgccagt gctccagaac
    34261 gcgtccggta gagagccaca ggtcgtactc ttcatccggt gcttccgccg ccggttcgaa
    34321 cggcagtgcg aagatcaccg ctttgccatc cggtttaccg tagaacttat agccttcgcc
    34381 cgctttcacg tacgggtcgt taccttcgct gtaacgccac tgtgtttctt taccattcac
    34441 caccggccag cgcagaccgc gcgctttgtg gtagtcatcg aacggtgcca gatcgtgacc
    34501 gtgaccgcga ccaaaccatg cgtactcttc gaacagccct ttttgcagat agaagcccag
    34561 ctcgcgggat tcatcgttca gctgatcttc cgccagttcg gataccggga atttgctcac
    34621 ttcgggggtg gcatacagaa cttcgtacag cgttttgcca cgcagttccg gtttcttcgc
    34681 cagcagctct tccggccata cttcttcagt tttgaagcgg cgggagaact ggactaactg
    34741 ccagagatcc gatttcgctt cgcctggcgc ctgtacctgc tgacgccaga actgagtacg
    34801 gcgttcggcg ttaccgtaag cgccctcttt ctctacccac attgcggtcg gcaggatcaa
    34861 gtcggcggcc agcgcactga ctgtcggata cggatcggag acgatgatga agttgcgcgg
    34921 atcgcgccag cccggcatac gctcttcatt gatgttcggc ccggcctgca tgttgttggt
    34981 acacatggtc cagtaaacat tcagcttgcc gtctttcagc gcacggtctt gtgccaccgc
    35041 atgaagaccg attttcgccg gaatggtgcc gctcgggata ttccacttct tctcacagat
    35101 atcacgatgt ttctcgttag ttaccaccat gtccgcaggc agacggtgag caaaggtgcc
    35161 cacttcacgc gcagtaccac acgcagaagg ttgcccggtc agggagaacg gaccgcaacc
    35221 cggctgggaa attttgccgg tcagcaggtg caggttgtag accaggttgt tggcccacac
    35281 accacgggta tgctggttga agcccatcgt ccagtaggag atgactttct tgttcggatc
    35341 ggcatacagc tgcgccagtt gttctaactg gtctttcggc acgccggtca tttcagcggt
    35401 tttttccagc gtatactcag caacgaaggc tttgtaatct tcaaagctca tcggttcgga
    35461 ggcgtcagaa cccggattct tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta
    35521 gccgatgtcc gtcgcccctt tgcgcaggtt aacgtgcttg ctgaagaagt cctgatttat
    35581 cgcattgttt tgaatgatat agttggcgat gtagttcagg atcaccaggt cagattgcgg
    35641 cgtaaagatg atgccgttat ccgccagctc gaagctacga tgctggtagg tagaaagcac
    35701 cgccacggtg acgttctggt tagagagacg acggttagtg atgcgtgacc agaggatcgg
    35761 gtgcatctcc gccatgttag cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc
    35821 atagcagccc atcggctcat ccataccaaa ggtacgcata aagccaacta ctgccgacgc
    35881 catacagtga cgtgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt tgaacagctt
    35941 ggacgcggca taaccttccc agatagtcca ctgaccagaa ccgaacatac cgatcgattc
    36001 cggccctttt tctttcaggg cggttttgaa tttctcttcc atcacatcga aggcctgatc
    36061 ccaggtgatt ggggtaaatt cgccttcttt gtcatattta ccgtttttca tacgcagcag
    36121 cggctgcgtc aaacggtctt taccgtacat gatcttgggc aggaaatagc ccttaatgca
    36181 gttcaggcca cggttaaccg gtgcgtccgg gtcgccctga caggccacca cacgcccctg
    36241 ctgcgttccg accagaacgc cgcaaccagt accgcagaaa cggcacggcg ctttatccca
    36301 tttaatggct tcctgctgac caacaacggc gcgggcaacg cccggcacgc tgagaccggc
    36361 agccgccgca gcggccgcaa cggcgttagc tttcataaag ctacgacgac tgagtttcat
    36421 ggtgtttcct caccttgctc ttcctgctgg tgataaacca gcgacaccgc cagcacgccc
    36481 tctacgttgc gtactgactc aatggtttgg atcagcgttt cgctgtcttc tgcttccacc
    36541 accacaatca actgaccgct cggcgcgtcg ctgacagcaa cttcacagcc gggaaaggcg
    36601 ttcagttggg tgctgatgtc tgaaattcgt tcgcttttgg cctgcacgac caggctgcaa
    36661 acttgccagt tagtgtgcat ggagatactc cgcagttatg gctgataccg gacagctggc
    36721 tgcacatgct ccacatccgt tgcaaagttg gctattaagt tgcgcctggt agatcccgga
    36781 gagcgtcggg cgaaagataa ttgccattgg ctcacagcta tcctgacagc ggcggcattc
    36841 aactgactga tacgccaggc acgcgtcccc gatggtgaac tgcaaatccc aggccctggt
    36901 gtggcgcgga gaaaatagcg attcggggca agcctgcgcg caggcgtaac agaagctgca
    36961 ctcgttattt ttgaaattaa cgctcggata gccacccgtg ccgcgttgca gaatgttgtt
    37021 ttcacaagca ttaatacagg cgtcgcagcg ggtgcaatgg gtcagaaaat gagattcatc
    37081 accggaccag ggcgggcgga taccgttact ggctttgcgc cagcgaccag tgagtatgcc
    37141 ccgacgggat gcatcaatct tcacattgac cttccatcat taacgcgctc tgaaaattga
    37201 gagcgaccaa atagaccgca taattaataa gccattttta tagccgctaa gatattaaag
    37261 gatgtgtcaa agatgcatac cccgatcggg gtaaaacctg cagcaggatc aaaagagtgg
    37321 cgggaagcgt ggcaaaaacg ggcttttgct cacatttcaa atgtttataa acatatttat
    37381 atagcgatta attcaccaga gatatttctg ctggtttgct ccctcatcag aatttaacac
    37441 taaaagagca ggtaaaattg tctgaatgtt ctttaagtta ttcataaagc aaattaataa
    37501 atctgatgaa tatgttaacc ttcagcgaca tcatcggtga aaacctataa atgaagaagg
    37561 aaagcaaaaa aatgaagacc attctacctg cagtattgtt tgccgctttc gctaccactt
    37621 ccgcctgggc ggcagaaagc gtccagccac tggaaaaaat cgcgccttat ccacaagctg
    37681 aaacaggcat gaagcgtcag gtgattcagt taaccccgca agaagatgaa tctaccctga
    37741 aagtagaact gttaatcggt cagacgctgg aagtcgattg caatttgcat cgtctcggcg
    37801 ggaagctgga aagcaaaacg ctggaaggct ggggctatga ttattatgtc tttgataaag
    37861 ttagctcgcc ggtttccacg atgatggcct gcccggatgg caagaaagag aagaaatttg
    37921 tcaccgcgta tctgggcgat gctggaatgc tgcgttacaa cagcaagctg ccgatcgtgg
    37981 tgtatacgcc agacaatgta gatgtgaaat accgcgtctg gaaggcggaa gagaaaattg
    38041 acaacgcgga agttcgctaa actgttgtga agtacggcac cccgttggtt ggataaggcg
    38101 gttacgccgc atccaacatc taacgcccga gccggttgcc tgatgcgacg ctggcgcgtc
    38161 ttatcagtat acgccacatc cggcatacca tgccggatgt ggcacatcat tacaacgcaa
    38221 tatccgccac ttctttttga acgggttgcg gtttcaactg cggtttcggc gtactatctg
    38281 ctgcttgcgg tttgtcgtat ttcagcccca gcacttcgct ggtgtactgc aactcgcgtt
    38341 ctgttgccgc tacatcaccg ttcagcttgc gtccataaga cggaacgatc gctttcaacg
    38401 tagcctgcca ttgcgggctg gaaacgcgat cgccaaatac tttttccagc agattcaaca
    38461 taatcggcgc ggcggttgac gcccctggcg atgcccccag cagcgccgca atggtccctt
    38521 gctggtcact gaccacttcg gtacccagac gcagtacgcc acctttctcg gcatcacgct
    38581 tgataatctg cacacgctgc cccgcttgcc acaaacgcca gtcctctttt ttcgcttgcg
    38641 gatagtactc tttcaacgct tcaaaacgat cctcttcact caacatcacc tgactcacca
    38701 gatatttcac cagctcgaaa ttatccagcc cgacgtgcat catcggcatc acgttagagg
    38761 tggtggtgga actcatcaga tcccacaatg aaccgttttt gaggaatttg gttgagaagg
    38821 tggcaaatgg cccaaacagc actacgcgtt taccgtccag aacacgggta tcgatatgcg
    38881 gaaccgacat cggtggtgcg ccaacggatg ctttaccgta aactttcgcc agatggcgat
    38941 taaccacgtc tggattttcc gaaacaagga actgtccgcc caccgggaaa ccggcgtagt
    39001 ctttcgcttc cggaatcccc gattcctgta acagcttcag cgccgcaccg cccgcgccga
    39061 taaagacaaa tttggtgcgg atgttctgtg cagtgccgtt tttcagatcg gcaacggtaa
    39121 cggtccaggt gttatcgtca ttacgcttta gggcgcggac ttcgctgctg agttgcagcg
    39181 agaagttaga tttcttctgt aaggaagcaa ttaactggcg ggtgatctca ccatagttca
    39241 catcagtgcc aatttctgtt gcggatcgcg cccttccatc actaacggtg cccactcttt
    39301 gatctgcgcg tgatcttcag agtaacgcat accgcgaaac agcgagcttt gttgcaacgc
    39361 ggcgtaacgg gcgcgcagga aattaacgtt atcctcgccc cagacaaagc tcatatgcgg
    39421 aacggtattg ataaatgaac gcggagtacg cagcacgccg cgctcgacct ggtgcgccca
    39481 gaactggcgg gaaatctgaa atgcttcgtt aatggcgact gctttttcaa tactgatgct
    39541 gccatcggcg ttttgcgggg tgtagttcag ttccatcagt gcagaatgcc cggttccggc
    39601 gttgttccag ccgttcgaac tctcctgcgc gcccccctcc aggcgctcca ccatggtcat
    39661 cgaccattca ggctccagct cgcgtaaata ggtccccaac gtggcgctca taatgccgcc
    39721 gccaatcaac agtacatcag tttcctgctc ctcggacgct ttcgcttttg ccgccatcga
    39781 aacggcatta agccccacgg ccatcgagaa gagcatggca gtcacttttt tcatcttgtt
    39841 aatgccttac ttttagtcgc tttattgcag gtgagatttg cgcggcatca acggtaacac
    39901 ttaagtaaca acatttaaat aatgtttata aattatattc taattttaga aacattataa
    39961 tttcgttgat taattatagg gttattagaa atgaagggat ttttagcata ttgacctgac
    40021 ggcagcagtc cgccaggtca gcgtcgtaat attatgccgt ccgggcaacg gcatcacgcg
    40081 aagcggcatc gcgctcttcg cccgtcagct cgctaagttg cccattgcgc atttccagca
    40141 ggcggtcggc gtggataaag taatgatcat catgactgat agcgaaaata gttttaccca
    40201 tctcctgcat cagcggcagc aacacctgat aaaactcacg acggaagtgt ggatcctgat
    40261 ccgccgccca ttcatccagc aggataatat cgcgttcttc tgccagcgcc agcaacagcg
    40321 gcacgcgttt tttctgccct tttgataact tcaggttaac aatacgcccg ttgcttaact
    40381 caagcttatg agccattttc agctgcgcca gccacttctc aaccagttgc gggttagcgg
    40441 gttgcccctc cggccccagc agttgatcaa acagccagac atcggtaaac actgccgaaa
    40501 acagtttgcg ataatcttcc ggttgttcgc cgctgacagg tttgccatcc agcaagattt
    40561 cgccgctttg tggctgatac aagcccgtca acaacatcgc cagcgtcgat tttccgctac
    40621 cgttgccgcc aatcagaaac agcagctcgc cacgtttgat ggtgagatta atcggaccaa
    40681 cggaaaacgc gttatcctga taagcaaacg tcacgttacg cagctccagc gtttgccagt
    40741 tgggaaacgc atgcgggcgc ggaaactcag ctttgaaagg cgcgagcgcg aatttgttca
    40801 gcttgttaaa cgccacctgc gccgtcagca gcgtcggcaa tgcgccaacc gccgaaagca
    40861 gcggcgtacg caggaataac agcgtcaacg aataggtcgc agcaacgttg gtatcagccc
    40921 aaccgagact gttcgccatc cagaacacca ggccgatagc gcccagcatc atgatgtttg
    40981 accagttcac ggcactaaga tggaaggtgt ctgcgcgaat aatatgatgg cgatactctt
    41041 gcgcatcggg gatgtagagg ttgttaaaca catactcggc gcgttcccgg ttcagagtca
    41101 gctctttgcg cccttcaagt acagtttgaa aatccgtgta cagcttgtct tcggtttcac
    41161 gtagggtcgc catatgtttg tacacccgcg ccaccagcac aaaaccgccc cagatggtga
    41221 tcgccatcca gatagccgtt accagcaaca ttttgcccga cagcatccac agatacgccg
    41281 atgaaccgat agtgagaatg atcccctgcc ccagttccgg cagacgcaca aaagcaatgg
    41341 tgatattgcg cacatcgctg gttaatcccg ccagcaacga ggcgctaccg agttgttcaa
    41401 tgcgctcgac gtgggtatcc agaatccgct tgataaattc actacgcagt cggtaaacga
    41461 agtgatgccc caaagtggtg agcgccagtt gcgatccgag agtgactgcc atcaacagca
    41521 gcaataatcc cagaaactcc ggtaacacca gcagactggt atccgccgtt tcgataaggc
    41581 gctgattgat aaaagcaatt aagccaatgc ctaatgccgc actggcgagg cttagcgcca
    41641 tcacactgat aaatggccag cgatactgcc gccagacaag tacaagaagt tccatgcaga
    41701 aaacccggac aatgaattac agcccgcagt ttaaacatct tcgcgcgcac agcaataata
    41761 attcttattt ttattctttt ttacctgcct gacggaatgt caggttgtag cggcagtcgg
    41821 tggtgagtgg atgaaacccc gctttcaacg gttgaatacc gtgataaaac agccgcgatt
    41881 caccgcccca ggccaccaaa ttgaaaaatc gcgggtaagc ccagagaaac agaaacaatt
    41941 ggcgcgcgca gatccggttc gtctttatcc tgatgcagcg acagtttcgc gccaggagcg
    42001 tagcggttga taagacaagc atctggctgg aaatctggat agcccgccgc cgtagccgca
    42061 cgttgacata aatcatgaaa gctttgtggc atggcgggcc acggtttatt tgtttgcgga
    42121 tcaatgggcg aatagagata accttgccga tgggtcgtcc agcccagatg cccacagttg
    42181 gtcatcgcca ccgacatggt atatccgcct ggagcgacca tctggcgaaa cggcgactgg
    42241 ctggcaacgt cattaatatc gcggattagt tgctccgcag cattaaaagc aaaacgccgt
    42301 aaaattaccg caccagccgc cagtggctct tgccacggtt cagcatcggc aaacagatcc
    42361 aacattacct ctcctcattt tcagcttcgc ggcgcagcag ttgcgcttta cgcgacacgc
    42421 cccagcggta accggaaagt gtgccatcac cacggaccac tcgatgacag ggtattacga
    42481 tagccagctt gttggcggca caggcgctgg caacggcccg taccgctttc ggtttgccga
    42541 tggcgttagc cagttgctga taactgacgg tttcaccaca aggtatcgtg cgcagtgcct
    42601 gccagacttg ttgctgaaaa gcagtgccgc gaatatccag cggtaacgtc agcggcgtat
    42661 cgcgttgatt gaggctggcg atcacttcac gcacatgttg ctgaaacgtc agatcggcag
    42721 gcgcgttgtc ggcagcggga aacatctgct gcaactcgct gattaatgtc gcgtcatcat
    42781 cgcccagcaa tatcgcgcaa atcccccgct cgctttctgc caccaggcaa cgacccagct
    42841 cacaatcagc cagcgcgtaa cgcaccgcca gattttcgcc accgtgacgg aattgtttag
    42901 ccgtcatgcc cagcgtttca tcggctttgc ggtagtaact gctgctgtcg gggaatccgg
    42961 cgttaagaat agacgtcgtc acgctctccc ctttcgccag cgattcgcgc aaacggcgag
    43021 cgcgccaggc ctgttgccag gctttaggcg tcattccggt agtagcttta aacaaccgat
    43081 gtagatgaaa tggactcatc gccacctggt cggctaaggc ttccagcgtt acaggcgttt
    43141 cctgttccag cagtcgacac gcgtgggtga ttttatccaa ccgatgttgc cggggattgg
    43201 ctttgtctgg ctgacaacgt ttgcaggggc gaaatccggc ggcgagtgcc tcgctggcat
    43261 ttgcgtagaa ggagacattt tcccgcaagg catgtctggc gcggcaagac ggacggcaaa
    43321 agatacccgt ggtgcgcacg gcgaaaacga attcgccgtc ggcattcggg tcgcgggcta
    43381 agacagattg ccagcgttga tcgtcagtta agcatgtggc gtttttcata atcagctccc
    43441 tggttaagga tagcctttag tctgcccggt caccatcacg caaaaaccaa caatcttgcg
    43501 ctttaatttt tttcgctgac aaggaagctt ttaaactgtg gtgacatcca ggttttaaag
    43561 ctatcgcctt ctttggtgat catataaacc gccagccctt cccggcgaac aacctctttg
    43621 gctttctccg gcccgagtac catcaagcca gtatcccagg catcggcttc cagcgccgtc
    43681 ggagcaatca ccgtcacgga taccagattg tgttcgatgg gacgcccggt ttgcggatcg
    43741 ataacatggg aaagacgttt gccgtccagt tcgtaatagt tacggtagct gccagaggtg
    43801 ctaatcccat gaccgttgat atccaccaca gcctgaaccg cgttttcttt atcggttggt
    43861 ttttgaatcg ctacccgcca cggctggcct tcaccgttca taccacggct gttcagcgcg
    43921 ccgcccaccg acaccagata gcgggaaatc ccttcctgct ccatcaagcg tgccaggtga
    43981 tccgccgcat aaccttcacc gacggtggag agatcgacat ataaatccgg caggtctttt
    44041 tgcagatatt gctgatgcga ctgattgatg accgtcaggt gctgtaagcc ggttttggct
    44101 ttcatcgcat cgatctgttc ctggctcgga atttgaaccg gctgttgttc cgggccaaag
    44161 ccccacagat tcaccagcgg cccgacggta atatccatcg caccatcggt ccttgcccca
    44221 atgcgcagcg aggtggtgac gatatcggcc atcgcttcac ttaccggcca cggcgacaaa
    44281 ctttgcgagt cgttaaagcg catcagcgcg gaatcttttt tataggtcga aagcagctga
    44341 tcgtcggcgt ccagctgggt ctgaatcttt tctttaagtt cggcactgcg tttggcatca
    44401 atgcccggga tgctggcacg ccagaaggta cccatggttt tgccttcaag aacggtaact
    44461 tcagtagcgt gggttttggc gggttgtggt ttttgatcgc aaccaacaaa gaagagcgcg
    44521 gcaaccagca gtgccacgcg ggtaaagctt atttccatac gtgattatcc tcatgcgaac
    44581 ggtcgcaaga gtacaccaaa aaactgtgtt tgtacgctga aaacaatgaa aaaagggccc
    44641 gcaggccctt tgttcgatat ccatcgagat tagaactggt aaaccagacc cagagctacg
    44701 atgttatcag tgttgatgcc agcgtcacga gtgaactggt tgtcgtccag caggttgatt
    44761 ttgtagtcaa cgtaggtgga catgtttttg ttgaagtagt aggtcgcacc aacatcaaca
    44821 tatttcagga tatcttcgtc gtcgtagtta cgaccattga tgacacccag gtttttacct
    44881 ttagactgca ggtaagctag ggacggacgc agaccgaagt cgaactggta ctgagcaaca
    44941 gcttcgaagt tctgtgcttt gttcgcccaa cccaagttgc ctacgcgagt tgcgttgtag
    45001 gtctgggtgt actgagcagc taggtagatg ttgttcgcgt cgtatttcag accaccggtg
    45061 taggtttcag cgcggtcgcc attaccgatg taacgctcat cataacggtt cagaccgaag
    45121 ttctggtcat cagtacgttt ggagctggaa actgcagcac cgataccgaa gccttcgtaa
    45181 tcataagtaa tagaaccgcc aacgccgtca ccgttctgac gcagagcttc acgaccgttg
    45241 ttggtcatgc cttcaccttc cgggctaccg tttttgccct gatactgaac agcgaagttc
    45301 agaccgtcaa ccagaccaaa gaagtcggtg ctacggtagg ttgcgaagcc gttaccacgc
    45361 tgctgcatga agttgtcaga accgtaagtg tcaccaccga attctggcag tacgtcggtc
    45421 caggaagtta cgtcgtaaac tacgccgtag ttacgaccgt agtcgaaaga acctacatcc
    45481 tggaatttca gacctgcgaa tgccacacgg gtccaggagt tgttttcgtt ttcagcgctg
    45541 ttgccctgga tctgatattc ccactggccg taaccggtca gctggtcagt aacctgagtt
    45601 tcacctttga agccaagacg catgtaggtc tggtcgccat ctacagactt gtcgtcagag
    45661 aaatagtgca ggccgtctac tttaccgtac agatctaatt tgttgccgtc tttgttgtaa
    45721 acttcagcag cgtttgctgc tcctgctacc agcagagctg ggaccaggag ggacagtact
    45781 ttaactttca tgttattaac cctctgttat atgcctttat ttgctttttt atgccactgc
    45841 atactgatta accctcatta atcagtcggc aagtccattc tccccaaaaa tgcagaataa
    45901 tccaacacga atatgatact aaaactttta agatgtttca tttatcgcta tagatgtttc
    45961 aaaatgtaaa tacaagggaa ctttttaaga ttattgcgga atggcgaaat aagcacctaa
    46021 catcaagcaa taataattca aggttaaaat caataactta ttcttaagta tttgacagca
    46081 ctgaatgtca aaacaaaacc ttcactcgca actagaataa ctcccgctat catcattaac
    46141 tttatttatt accgtcattc atttctgaat gtctgtttac ccctatttca accggatgcc
    46201 tcgcattcgg tttttttacc cttctttaca cacttttcat tattctgtgc taccacagaa
    46261 aaactataac gcttgttaac tatttcacaa ataattaaca tcagcataat ttccagcaat
    46321 ctttgtttat ttacaattaa tttcgttggg ctttttgtag gttatttgta cagcaaaatg
    46381 gcgcttgtac atctatttcc ccccatacag gatgataaat atcacgggag aatagagaat
    46441 catcaatcag gtaagagtct ggaatttcac actgtaccct ttatactgcc ctatcacttc
    46501 gcgaagtttt aacaggtcat aaacacgaat gcgtcagaaa gagacaacgg ccacgacccg
    46561 cttttcactc ctaccgggga gcattacccg cttcttttta ctgttgatca ttgtgttact
    46621 ggtgacgatg ggggtaatgg tacaaagtgc cgttaacgcc tggctgaaag ataaaagtta
    46681 ccagattgtc gacattaccc acgcaatcca aaagcgcgtc gatacctggc gttacgtgac
    46741 caggcagatc tacgacaaca ttgccgcgac gacctccccc tcctccggcg aaggtttaca
    46801 agagacgcgc ctgaaacagg atgtctacta tctggagaaa ccacgccgca aaacggaagc
    46861 gttaatcttt ggctctcacg acaactcaac gcttgagatg actcagcgga tgtccactta
    46921 tctggataca ttgtggggtg cagaaaatgt accgtggtcg atgtattacc tgaatggtca
    46981 ggataacagt ctggtgctga tctcaaccct accccttaaa gatctcacct ccggatttaa
    47041 agaatcgacc gtcagtgaca ttgttgattc acgtcgtgca gaaatgttgc aacaggccaa
    47101 cgccctcgat gaacgcgaaa gtttttctaa catgcgccgc ctggcctggc agaacggtca
    47161 ttactttacc ttgcgtacca cgttcaacca gccgggacat ctggcaacgg tcgtggcttt
    47221 tgatctgccg attaatgatt tgatcccacc gggtatgccg ctggacagtt tccgccttga
    47281 gccagacgcg acggcaacgg gaaacaatga taatgagaaa gaagggacgg atagcgtcag
    47341 tatccacttt aacagtacga agattgaaat ctcctcggca ctcaactcta ccgatatgcg
    47401 cctggtctgg caggttcctt atggcacctt attgctggat acgttgcaaa acattctgct
    47461 gccactgctg ctgaacatcg gtttgctggc gctggcgtta tttggctata ccacattccg
    47521 ccatttctcc agccgcagta cagaaagcgt ccccagcacg gcggtcaata acgaattgcg
    47581 cattttacgg gcaattaatg aagagatagt ctcactgttg ccgctcggcc tgctggttca
    47641 cgatcaggaa tcgaaccgca ctgtcataag taacaaaatt gccgatcatt tgctgccgca
    47701 tctgaatctg caaaacatca ccaccatggc ggaacagcat caggggatta ttcaggcgac
    47761 gatcaataac gagctgtatg agatccgcat gttccgcagc caggtcgcgc cgcgcacaca
    47821 aattttcatt attcgcgatc aggatcgcga agtgctggta aacaagaaac tcaagcaggc
    47881 gcagcgtctg tatgagaaaa accagcaggg gcggatgacc tttatgaaaa acattggcga
    47941 tgcgctgaaa gaacccgcac agtccctggc ggagagtgcg gctaaactca acgccccgga
    48001 aagcaaacaa ctggcgaatc aggcagatgt gctggtgcgg ctggtcgaag aaatacagtt
    48061 agcgaacatg cttgcggatg atagctggaa aagtgagacg gtgctgttct ccgtgcagga
    48121 tttaattgat gaagttgtgc cttcagtgtt gcctgccatc aagcgtaaag gtctgcaact
    48181 gctgattaac aatcatctga aagcacacga tatgcgccgc ggcgatcgcg atgccttacg
    48241 acgtattttg ctgctactga tgcaatatgc cgtgacctca acgcaattgg gaaaaatcac
    48301 ccttgaggtt gatcaggatg agtcctctga agaccgcctg acattccgca ttctggacac
    48361 gggagaaggc gtaagtattc atgaaatgga taatttgcac ttcccgttta tcaaccagac
    48421 ccaaaacgat cgttatggaa aggcggaccc gctggcattc tggctgagcg atcaactggc
    48481 acgtaaactg ggcggtcatt taaacatcaa aacgcgggat gggcttggta cacgctactc
    48541 tgtgcatatc aaaatgctcg ccgctgaccc ggaagttgaa gaggaagaag agcgtttact
    48601 ggatgatgtc tgcgtaatgg tggatgttac ttcggcagaa attcggaata ttgtcactcg
    48661 cctgttagaa aattggggtg caacctgtat cacacccgat gaaagattaa ttagtcaaga
    48721 ttatgatatc tttttaacgg ataatccgtc taatcttact gcctctggct tgcttttaag
    48781 cgatgatgag tctggcgtac gagaaattgg gcctggtcaa ttgtgcgtca acttcaatat
    48841 gagcaacgct atgcaggaag cggtcttaca attaattgaa gtgcaactgg cgcaggaaga
    48901 ggtgacagaa tcgcctctgg gcggagatga aaatgcgcaa ctccatgcca gcggctatta
    48961 tgcgctcttt gtagacacag taccggatga tgttaagagg ctgtatactg aagcagcaac
    49021 cagtgacttt gctgcgttag cccaaacggc tcatcgtctt aaaggcgtat ttgccatgct
    49081 aaatctggta cccggcaagc agttatgtga aacgctggaa catctgattc gtgagaagga
    49141 tgttccaaga atagaaaaat acatcagcga cattgacagt tatgtcaaga gcttgctgta
    49201 gcaaggtagc ctattacatg aacaatatga acgtaattat tgccgatgac catccgatag
    49261 tcttgttcgg tattcgcaaa tcacttgagc aaattgagtg ggtgaatgtt gtcggcgaat
    49321 ttgaagactc tacagcactg atcaacaacc tgccgaaact ggatgcgcat gtgttgatta
    49381 ccgatctctc catgcctggc gataagtacg gcgatggcat taccttaatc aagtacatca
    49441 agcgccattt cccaagcctg tcgatcattg ttctgactat gaacaacaac ccggcgattc
    49501 ttagtgcggt attggatctg gatatcgaag ggatcgtgct gaaacaaggt gcaccgaccg
    49561 atctgccgaa agctctcgcc gcgctgcaga aagggaagaa atttaccccg gaaagcgttt
    49621 ctcgcctgtt ggaaaaaatc agtgctggtg gttacggtga caagcgtctc tcgccaaaag
    49681 agagtgaagt tctgcgcctg tttgcggaag gcctcctagt gaccgagatc gctaaaaagc
    49741 tgaaccgcag tattaaaacc atcagtagcc agaagaaatc tgcgatgatg aagctgggcg
    49801 tcgagaacga tatcgccctg ctgaattatc tctcttctgt gaccttaagt ccggcagata
    49861 aagactaatc atctgtaggc cagataagac gcgttagtgt cttatctggc atttgcaccg
    49921 attgccggat gcggcgtaaa cgccttatcc ggcctacgat tcccattatt tcaacagatt
    49981 acattaaagt aggccagata agacgcgtca gtgtcttatc tggcatttgc actgaatgcc
    50041 ggatgcggcg taaacgcctt atccgtccta cgaatcccgc gatttcctga ccctctcggc
    50101 atataccgtc agcgtctgtt ttatcacatc cagcgttacc ggcttcgaga ggcagctgtc
    50161 cataccggac tccagacacc gctgcttctc ttcagccaac gcattagcag ttactccgat
    50221 taccggcaac gtcagtccca actgacgaat gcgttgcgtc aagcggtaac catccatatt
    50281 tggcatgttg acgtcgctaa gcacgatatc aatatgattc ttactaagta cattaagcgc
    50341 atcgacgcca tcattcgcgg ttttacattg atagcccaac gatcccaact gatctgccag
    50401 cagacgtcgg ttaatcggat gatcatccac gaccagaatc atcatatcgt cgttatcgct
    50461 gaccgctttg tccgtcgacg gcagagcgtt agcaggatcg tcgctctcca tctcgatcaa
    50521 ataaatacgc gccaacaatg ccggtagctc atgcggagca gccacactgt gtacccactc
    50581 ccctggcgct ttctccagcg gaataccaat atggcgacga cagaaggtca ctaccgctct
    50641 gccctgccat tttttactca ctacctcgtc agtgattaac acatcttcgg gagtcggttc
    50701 ctgcccttcg tatgttgtaa cgacgatgcc gctgcgctgc aaactggttt ccaggaactg
    50761 acagagcgac gcattgcgga ccgccagcca gcagcgttta ccactcaacc cttccacgcc
    50821 ttttttctgc gggtactgag cgccgtacaa cggaatacgc acggtaaact ggctgcccat
    50881 tcccggttct gaatctaccg agatatcgcc gtccatcatg ctgatcagtt tttcacaaat
    50941 cgccagaccc agaccggtcc cctggaagtt tcgctgtacg cccgttccaa cctggaagaa
    51001 gggatcaaac aagcgcacca cttctttcgc cggaatcccc acgccggtat cgcgaacacg
    51061 gatagagaga taatcgccat cagcgcgaac atgcaaaact atacagccgg tatcggtgaa
    51121 ttttatggcg ttactcaaca ggttggagat gacctgctgt aaacgcatcg ggtcgccatt
    51181 taaggccact ggcacatccg gttcaataaa gcagtacaag cctaactgct tgcgtaccac
    51241 cagcggtaaa tagttggcgg tgatgtggtt catcacttca cgcggtgaaa actcacgcgg
    51301 ttcgatcttc aactgttccg attcaatctt cgagaaatcg agaatatcgc tgataatttt
    51361 caacaacagg ctggaagagt tgttcattgc cgtcaccaga cgatcgacgc ctttcggtaa
    51421 ctctttggtt tgcaacagat ccaggttacc gataatgcca tacagcggcg ttcgcagctc
    51481 atgactgacg gtggcaagga acatcgattt tgactggctc gcctgttccg ctgcttgtgc
    51541 catctcctgc aacgactctt ccatcttcac gcgcgaagaa acatccacca gcacacaaat
    51601 ggccacgttt tcattacgat agcgcgaatg gacgaagctg atttgcagat tggtattgtt
    51661 gctggtcagg acatcaacaa aattgacctg ctgcccacag ataatttgcg tcagtcgttg
    51721 gcggtcctca tgcgtaagca tattgagata ggtatgcgcc agttcgttac ttaaaatatt
    51781 gacgccatca gcggtacgca aaatgcagat acccactggc gcggaggcga caatcttgcg
    51841 attgaactgc tcatgttctt ccagtcgcag ggcgtcgctt tccgccggaa tgaaaatacg
    51901 tcgctcgtac atccgcgcga gagtaaacaa tgcagctccg gcaagcacat tcagcaaaat
    51961 tgcgttaagg atcaacatgc gaatgcgttc cagcacctta tcaaccggca ccgaatacac
    52021 gatgcttagc gatgagggtg gcagattttt cttcagcacc agctcccgga acccttccgt
    52081 atagccaaac caggagcgtt cctgcatcca gcgaggatcg cccttaattt tactttctgg
    52141 tccggtaagc gaaatcaggg tatgaccatt ttcatcaaga atggtaaccc ccatcggcaa
    52201 cgtacccggt aagaaaaagt tctccatccg gatggtctgc tcgacaccca aaagcgcctg
    52261 caaccggttc gccagataaa ctggcgtcaa cgcgtaaaaa tacccgacgc cagggcgcgg
    52321 accttcgctg atccagtaga ggttactgcc gctatcatct tgtggtgcat ttcgatattt
    52381 attgatgcgt tcatgcaacg ctttcaacgc ggtatcgcgt tccactggca tatcacgcag
    52441 accgaaattg gccatgcaga ggttatcact gccgattaaa aatacccggt tgagatcgta
    52501 agccgcagaa aaattatcgc gccagtagcg cataaaccac gccaatgact ccagagaacc
    52561 tcgccaggtg ttactcattg cggaacaatc ggagtcggca aacagcggtt caaacgcagg
    52621 cacatccgcc tgcgtttctc gtccacgcgg ggaaagcaca ccgttttctg ccgataagcg
    52681 attttcggcg atgtacttaa gctctttcat cacatcagag gtgcgttgaa taaagcgctg
    52741 agcctgatcg gaactcagat taaattcctg acgaatttcc gattctcgct gatgtaacgc
    52801 attaacgatg taaaaaacgg atgaaaaagc aatcaacagc cagagcacta acgccaatgc
    52861 tctgaacatg tagcgcgagg ctttcagggt tgtacgaaaa gaagcaaggt atttcaaagg
    52921 ggcgaagctc cgcctcaggt gaccgatgga gtgtggttaa ggtagcggta aaagcgtgtt
    52981 accgcaatgt tctgtcacca ttgcaataat gcgtatcgct tagaaaccat tgccttaacc
    53041 aggaaagccg gagtagatgg acagttctca gtacgaaaaa taagaaatga ccaatctgcc
    53101 gacgcttgtt aatggttata tcctggcgtc ggcagatcgc ccagcagtct tatctgaaaa
    53161 aagagagagc caggttgtgt gaagggatgg atcaccagtc aaaacttttc accagcgtca
    53221 gctcgccagc attacgcatc ggtacaataa atgtttcctg tttctcattg accgatcctt
    53281 catcggtgat cagcgtcagt tgggcggtcg tcaattccgt ttcgctgcgc ccaccatagt
    53341 agttgatata cacctgatag cgcccgtgaa ctggtgctgg catggcgaaa atctcgggtc
    53401 cgtaccccgt cgtgacatcc atatccagtg caccactgtt tttcagcacg gtgttaccgt
    53461 accaggcgtg ttcgccatcg ggcgtgacaa catggagatc gagatcggta ttgtccgtat
    53521 cccacgagag aaccagccgt aaacgtgcac gaatagttcc tgtacccggc gttgagtaaa
    53581 actgcatttt ttgccggctt tgcccatccg ggctgatgac ctgcacgctg ttgctgcctt
    53641 cagtgaaaat ataagggcgt gcaaatgaac catcggattc aattctctgc ggcatactgg
    53701 caccgttgac caccagccgg ccctgctgaa ctttacccgc cgcagcataa ttctttattt
    53761 tgccgctgat ctgcgctgag atattttgat catctgccat gttgacggac gacgccgggt
    53821 aattgatcga ttgtgaaaaa ctggcatctt cgccctctgc cggatgccag ccagagagcg
    53881 gtgcgtcaat ctcgacttcc tgcacacctt cactgtgagc gacaggcgaa agtgccacca
    53941 gtaacaacgg aagaaaaatc tttcgcatag cattagtcca ttaataattg tcgggtcagc
    54001 gtttcgatat agttctcatc aagcccggtg ggataaccat caaaggccaa gtgcagatac
    54061 tcatgggtta aatccagccg atcctgaagc gtaaagaagt tacgaataaa cagccgtttc
    54121 tgctgccgat cggtataggg gaaacctgag actaaacgac agaccgcaaa cacgtctggt
    54181 tcgttgtacc ccgtctcgcc ttgtaatata cgccgccact gcggcatttt tttcgccagc
    54241 caggctttcg ctttgggtaa taactggcag gttgagcgtg gagctcccca gcggctaagg
    54301 ctgttgtcgg gataagcaaa cgcgagtatc tggtcgtaac gctccccctg ccccgcctgg
    54361 gccgtggctt gccgccagga aagtgtccct tcggtagcgc gactgccatg ataatgaacc
    54421 ggatcacctg catagatgag ctcctgcgtc caggcggtca tcgttcgcgc ccccgtggtc
    54481 gctggcgaag cagaaacacg ctgcgtggcg ctgctgccgg ggatggtcag gcaatcacct
    54541 tcccgattgg cgttttgctg taaaaaagta cgtatcccca cagtcatggc tttcgctgcc
    54601 tcaggtggcg tacttttcgc ctcgcgatcc agaactctgg caacgtactc ttcacgatcg
    54661 agatgcgact gcaatttcag tttgcccttt tcgctcagta aggtcgtctc accgtggctg
    54721 acaaacgtaa tgtggttacc attagtaaac gtcacacgat agcgcccatt aagtacgctg
    54781 ggttttactg ccgtcgtact cttttccgct gtgattttct tcagcggata gcgggcaaac
    54841 agctccacct ctacacattg cccactggca acctgcgtcg gcactggcaa aacccgatta
    54901 agcaccgtcg cataacgggt tagtaccgtt ttgctggtgc cactgccagt aacccacagc
    54961 ggtgtaccat cggttaacca gccagcaaaa ccaccctgac ggatttcctg tttatcatcg
    55021 gcaaaccagc tccaggtttt cacccgtacc cgactaccga gcatggatga gacaccgatt
    55081 ttcgcctcgt ccagcaccac atccagtaag acttcctgag ctttgttttg cgcaggtaac
    55141 gtcgctaatg aatcaagcag cgatttaacc gttaccgagg tttctggctt cagcgttgta
    55201 agaaacgcta accacgcagg tgcctgacgc tgttgccagt attgccccca cacatctgcg
    55261 ccaatatgca accgctgcgg cgcaaaatag agcccacagg aacgtaccag cgcggtatcg
    55321 cgcgttatgc tctccccagc ctgacaacaa taaacctctt ccggggagtt tccgcgacac
    55381 tggtagggct gctccggttg atgggtatcc tccagccagg cgtaaacata cagtttccac
    55441 aaactcccta acggcgtggt gagcgagtca ggtaatgctg agactttggt cacgcctgat
    55501 gacgacagct gatacagttg atcgtgctgc gcaccgcgca gcaccagttg taacggcgtc
    55561 tcctccgcaa gcgttggcag agtaaccaac gccaatagcc agacgattct gcgccagttc
    55621 acttatttca cctgcatccc ggtccattca ctccctgccg cgacactttg ctgcgcaggt
    55681 gcataggaac gcacataacg agcaggaggc agaacgaatt gccctttttg cgagaagcgc
    55741 agcaaatggc ggaaagtgac cgttcccgtc agttctttca ccggcaccat ataggccagt
    55801 tcgcccattt cattacgcgc tttttccagc aattgcccct gctgtttcgc ggcgttgggt
    55861 ttattgactg aaatgcccca tgttgtgcgc tcaacgtcgg ctcccggcgg gagcggtact
    55921 tccacctgac catagcgcag aactgcatcc tgctcgcagg taagcgtaat ttcatcgaga
    55981 tacagcgcat cgctgtcaat ctcattgctg gtcaccggct gcagagtaaa gctcatctct
    56041 tcttcaccgg ggataagccg atacaactgg cgttcaacgg tcaccggaat gttactttgt
    56101 tgagccgttt ttgccggttc acgccagcgg acctgcacat tttgcggcga taattcgtca
    56161 ccaaaagaga gaatgtccgg cacaccctga ccaacccaac gccagtcttc gccccctcca
    56221 gttaacttat gtttagccca tgcgcccgca ggcgcaggca aaacaactgg aggcattgtc
    56281 gccatatatt tcgccagcca gttcatggcc agcgcgcgct caatagtgga ttgctcagcg
    56341 gtcaaaccat taaaatagcg gccgtctggg tagcgtcacc accgctattg agcagcacaa
    56401 ccgtgtgcgc cagcggctga ttgctgtttg ccgctttatc ccacgcatat tgctgaacct
    56461 gtggcatcac cgtggacttc aactgcgctt ttttcagcaa cgtcatggtt aagacgtttg
    56521 ccaccgcatc tgccagtgga gattccggtg tatcgaggat caggctgtca ttgatatcgc
    56581 ggatgtcttc ctcagagaaa tcttcatctt tagttccgcg ccgggcgata gcttcatccc
    56641 cccctttcaa caacgttttg cacggcagat tcatctcctg tgcccacgcc agcaccagcg
    56701 cccgatgcaa taacggcatg ttatccgcct gctcggcata actgtcgagc atatgctgcc
    56761 agtattccgg ttgttgcgtt acgccgagcg cctggctggc ctgccaatcg gcgtaccatg
    56821 cccatgccgt aaggaaggcg tcaccattgc catcttcacc ccaccaggta aagcgcgctc
    56881 cgggccccgc cagttgcatc agccgcagac ggttatcctg aatcatctga cgaatgtcgt
    56941 tagcggcggc actttgatga tcggcaagcg aacgccaggc gagactgagc gggatcagac
    57001 ggctaccggt gttgatcacc ccaccccacg gttcatcgac taacgcatca agattgttgc
    57061 ggaaaatctc ctgcggcgtt tcactacttt gtagccggat attactcgcc tgctcgggca
    57121 acatcagcgc gttctcgcca ccgccgagca tgacattttt ctgctgttca acgggccagc
    57181 tgttgtccac aaaagacagt tttgtgctaa tgctgtcctg cacttgccca ttttgttgca
    57241 gttctgcact taacaagcca gattgctgaa tattctgcgt cagcgaaata taattcgccc
    57301 ctttgtgcag cgtcagcgtc tggcgcatct cagcgcctgc aaatttagtc accagcgcca
    57361 ccggttcgtt atcctgctga ctgaagataa acagtcctgc cgccggtttg tcgcccacgc
    57421 gatacaccgt tggcatactc cacttcatgt agagattttt ttccgaacgc agataagcac
    57481 gcccctgccc gaccagcccg tcgccgttca tcccacgcgc ggtgatacgc cagcgggtta
    57541 acgaatcagg catcaggaac gtgaaatacg ctttgccttg tttatcggtt gtgagtgacg
    57601 gcatccatgc cgcggtatcc acctcttcac gccgtggacg ttcaagcatt tttactcgcc
    57661 gctcgctgcg gttagtcgcg ccaggcgcaa ccggctcgct ggagagcgcc tggtcatagc
    57721 tgataaacga cagactggag ctggtacgca cattgttacg ccccagcgga tagaaaaatt
    57781 tgccgatatt cggcgcgatt tctggttgca gcgcgtagat catttcatcg accacgccta
    57841 ccgttagctg cgcagaaaca ggtttacctt tcagcgacga ggttaattct gaagtggtca
    57901 acaaaaactg gccacc
//
LOCUS       DAJTYG010000015        55768 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTYG010000015 DAJTYG010000000
VERSION     DAJTYG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.015"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            79..867
                     /gene="modE"
                     /locus_tag="OO568_000968"
     CDS             79..867
                     /gene="modE"
                     /locus_tag="OO568_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6640421.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIAALC"
     gene            935..2407
                     /gene="modF"
                     /locus_tag="OO568_000969"
     CDS             935..2407
                     /gene="modF"
                     /locus_tag="OO568_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6640422.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            2668..3684
                     /gene="galE"
                     /locus_tag="OO568_000970"
     CDS             2668..3684
                     /gene="galE"
                     /locus_tag="OO568_000970"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6640423.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            3694..4740
                     /gene="galT"
                     /locus_tag="OO568_000971"
     CDS             3694..4740
                     /gene="galT"
                     /locus_tag="OO568_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6640424.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNETEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            4744..5892
                     /gene="galK"
                     /locus_tag="OO568_000972"
     CDS             4744..5892
                     /gene="galK"
                     /locus_tag="OO568_000972"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6640425.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            5886..6926
                     /gene="galM"
                     /locus_tag="OO568_000973"
     CDS             5886..6926
                     /gene="galM"
                     /locus_tag="OO568_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6640426.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYTNRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            7129..7881
                     /gene="gpmA"
                     /locus_tag="OO568_000974"
     CDS             7129..7881
                     /gene="gpmA"
                     /locus_tag="OO568_000974"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6640427.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(8048..9100)
                     /gene="aroG"
                     /locus_tag="OO568_000975"
     CDS             complement(8048..9100)
                     /gene="aroG"
                     /locus_tag="OO568_000975"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6640428.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            9416..9796
                     /locus_tag="OO568_000976"
     CDS             9416..9796
                     /locus_tag="OO568_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6640429.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            9910..10851
                     /gene="zitB"
                     /locus_tag="OO568_000977"
     CDS             9910..10851
                     /gene="zitB"
                     /locus_tag="OO568_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6640430.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(10848..11567)
                     /gene="pnuC"
                     /locus_tag="OO568_000978"
     CDS             complement(10848..11567)
                     /gene="pnuC"
                     /locus_tag="OO568_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6640431.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(11605..12648)
                     /gene="nadA"
                     /locus_tag="OO568_000979"
     CDS             complement(11605..12648)
                     /gene="nadA"
                     /locus_tag="OO568_000979"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6640432.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKTRADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAVTLRG"
     gene            complement(12897..12972)
                     /locus_tag="OO568_000980"
     tRNA            complement(12897..12972)
                     /locus_tag="OO568_000980"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12937..12939),aa:Lys,seq:ttt)
     gene            complement(12976..13051)
                     /locus_tag="OO568_000981"
     tRNA            complement(12976..13051)
                     /locus_tag="OO568_000981"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13016..13018),aa:Val,seq:tac)
     gene            complement(13103..13178)
                     /locus_tag="OO568_000982"
     tRNA            complement(13103..13178)
                     /locus_tag="OO568_000982"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13143..13145),aa:Lys,seq:ttt)
     gene            complement(13181..13256)
                     /locus_tag="OO568_000983"
     tRNA            complement(13181..13256)
                     /locus_tag="OO568_000983"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13221..13223),aa:Val,seq:tac)
     gene            complement(13393..13468)
                     /locus_tag="OO568_000984"
     tRNA            complement(13393..13468)
                     /locus_tag="OO568_000984"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13433..13435),aa:Lys,seq:ttt)
     gene            complement(13633..14424)
                     /gene="cpoB"
                     /locus_tag="OO568_000985"
     CDS             complement(13633..14424)
                     /gene="cpoB"
                     /locus_tag="OO568_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6640433.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(14434..14955)
                     /gene="pal"
                     /locus_tag="OO568_000986"
     CDS             complement(14434..14955)
                     /gene="pal"
                     /locus_tag="OO568_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6640434.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(14990..16282)
                     /gene="tolB"
                     /locus_tag="OO568_000987"
     CDS             complement(14990..16282)
                     /gene="tolB"
                     /locus_tag="OO568_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6640435.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(16415..17656)
                     /gene="tolA"
                     /locus_tag="OO568_000988"
     CDS             complement(16415..17656)
                     /gene="tolA"
                     /locus_tag="OO568_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6640436.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(17721..18149)
                     /gene="tolR"
                     /locus_tag="OO568_000989"
     CDS             complement(17721..18149)
                     /gene="tolR"
                     /locus_tag="OO568_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6640437.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(18153..18845)
                     /gene="tolQ"
                     /locus_tag="OO568_000990"
     CDS             complement(18153..18845)
                     /gene="tolQ"
                     /locus_tag="OO568_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6640438.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(18842..19246)
                     /gene="ybgC"
                     /locus_tag="OO568_000991"
     CDS             complement(18842..19246)
                     /gene="ybgC"
                     /locus_tag="OO568_000991"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6640439.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQHIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(19396..19689)
                     /gene="ybgE"
                     /locus_tag="OO568_000992"
     CDS             complement(19396..19689)
                     /gene="ybgE"
                     /locus_tag="OO568_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6640440.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(19689..19802)
                     /gene="cydX"
                     /locus_tag="OO568_000993"
     CDS             complement(19689..19802)
                     /gene="cydX"
                     /locus_tag="OO568_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6640441.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(19817..20956)
                     /gene="cydB"
                     /locus_tag="OO568_000994"
     CDS             complement(19817..20956)
                     /gene="cydB"
                     /locus_tag="OO568_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6640442.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTGGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(20972..22540)
                     /gene="cydA"
                     /locus_tag="OO568_000995"
     CDS             complement(20972..22540)
                     /gene="cydA"
                     /locus_tag="OO568_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6640443.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILIANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(23389..26094)
                     /locus_tag="OO568_000996"
     CDS             complement(23389..26094)
                     /locus_tag="OO568_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR6640444.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGFRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLEPLEPLEPLEPLEPLEPLEPLEPLA
                     TLAWTLGFEYHHGLLEKIWKEILKNHAHDSIGCCCSDKVHREIVARFELAEDMADNLL
                     RFYMRKIADNMPQSDADKLVLFNLMPWPREEVINTTVRLRASQFNLRDDRGQPVPYFI
                     RHAREIDPGLIDRQIVHYGNYDPFMEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKS
                     DAEGILENAFWQIALNEDGTLQLVDKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWV
                     ITSANAKPQCEIIHEAWQSRAVIRYEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNR
                     RIDVDINLDNQADDHRLRVLIPTSFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKE
                     APVPVWNMLNYAALQEGRNGMAVFSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLL
                     LRPRRPSGIKMPVPDSQLRGLLSCRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIP
                     WDAMKLNKAGFNVPESYSLLKMPPVGCLISALKKAEDRQEVILRLFNPAESATCDATV
                     AFSREVISCSETMMDEHITTEENQGSNLSGPFLPGQSRTFSYRLA"
     gene            complement(26112..28028)
                     /gene="mngA"
                     /locus_tag="OO568_000997"
     CDS             complement(26112..28028)
                     /gene="mngA"
                     /locus_tag="OO568_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6640445.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWESVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYLVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMQ"
     gene            28198..28920
                     /gene="mngR"
                     /locus_tag="OO568_000998"
     CDS             28198..28920
                     /gene="mngR"
                     /locus_tag="OO568_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6640446.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(29024..29893)
                     /gene="sucD"
                     /locus_tag="OO568_000999"
     CDS             complement(29024..29893)
                     /gene="sucD"
                     /locus_tag="OO568_000999"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6640447.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(29893..31059)
                     /gene="sucC"
                     /locus_tag="OO568_001000"
     CDS             complement(29893..31059)
                     /gene="sucC"
                     /locus_tag="OO568_001000"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6640448.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(31153..32370)
                     /gene="odhB"
                     /locus_tag="OO568_001001"
     CDS             complement(31153..32370)
                     /gene="odhB"
                     /locus_tag="OO568_001001"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6640449.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(32385..35186)
                     /gene="sucA"
                     /locus_tag="OO568_001002"
     CDS             complement(32385..35186)
                     /gene="sucA"
                     /locus_tag="OO568_001002"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6640450.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(35487..36203)
                     /gene="sdhB"
                     /locus_tag="OO568_001003"
     CDS             complement(35487..36203)
                     /gene="sdhB"
                     /locus_tag="OO568_001003"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6640451.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(36219..37985)
                     /gene="sdhA"
                     /locus_tag="OO568_001004"
     CDS             complement(36219..37985)
                     /gene="sdhA"
                     /locus_tag="OO568_001004"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6640452.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(37985..38332)
                     /gene="sdhD"
                     /locus_tag="OO568_001005"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(37985..38332)
                     /gene="sdhD"
                     /locus_tag="OO568_001005"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6640453.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(38326..38730)
                     /gene="sdhC"
                     /locus_tag="OO568_001006"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(38326..38730)
                     /gene="sdhC"
                     /locus_tag="OO568_001006"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6640454.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            39111..39218
                     /locus_tag="OO568_001007"
     CDS             39111..39218
                     /locus_tag="OO568_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640455.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            39424..40707
                     /gene="gltA"
                     /locus_tag="OO568_001008"
     CDS             39424..40707
                     /gene="gltA"
                     /locus_tag="OO568_001008"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6640456.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            41098..41663
                     /locus_tag="OO568_001009"
                     /pseudo
     CDS             41098..41663
                     /locus_tag="OO568_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            41714..44173
                     /locus_tag="OO568_001010"
     CDS             41714..44173
                     /locus_tag="OO568_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6640457.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWYSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            44188..44919
                     /locus_tag="OO568_001011"
     CDS             44188..44919
                     /locus_tag="OO568_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6640458.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            44916..45977
                     /locus_tag="OO568_001012"
     CDS             44916..45977
                     /locus_tag="OO568_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6640459.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIHVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            46129..47175
                     /locus_tag="OO568_001013"
     CDS             46129..47175
                     /locus_tag="OO568_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6640460.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(47172..47963)
                     /gene="nei"
                     /locus_tag="OO568_001014"
     CDS             complement(47172..47963)
                     /gene="nei"
                     /locus_tag="OO568_001014"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6640461.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(47999..48733)
                     /gene="pxpA"
                     /locus_tag="OO568_001015"
     CDS             complement(47999..48733)
                     /gene="pxpA"
                     /locus_tag="OO568_001015"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6640462.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKETQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(48723..49655)
                     /gene="pxpC"
                     /locus_tag="OO568_001016"
     CDS             complement(48723..49655)
                     /gene="pxpC"
                     /locus_tag="OO568_001016"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR6640463.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(49649..50305)
                     /gene="pxpB"
                     /locus_tag="OO568_001017"
     CDS             complement(49649..50305)
                     /gene="pxpB"
                     /locus_tag="OO568_001017"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6640464.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(50328..51071)
                     /gene="ybgI"
                     /locus_tag="OO568_001018"
     CDS             complement(50328..51071)
                     /gene="ybgI"
                     /locus_tag="OO568_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6640465.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLENDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            51342..52823
                     /gene="dtpD"
                     /locus_tag="OO568_001019"
     CDS             51342..52823
                     /gene="dtpD"
                     /locus_tag="OO568_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6640466.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTLF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(52973..54391)
                     /gene="phrB"
                     /locus_tag="OO568_001020"
     CDS             complement(52973..54391)
                     /gene="phrB"
                     /locus_tag="OO568_001020"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6640467.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(54388..54897)
                     /locus_tag="OO568_001021"
     CDS             complement(54388..54897)
                     /locus_tag="OO568_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6640468.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            55026..55343
                     /locus_tag="OO568_001022"
     CDS             55026..55343
                     /locus_tag="OO568_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR6640469.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSLHLK
                     GDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELREELYQVKQVVKGMRDGMFSVL
                     NES"
     gene            complement(55398..55718)
                     /locus_tag="OO568_001023"
     CDS             complement(55398..55718)
                     /locus_tag="OO568_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640470.1"
                     /translation="MPGYRTWIEISLIDELKPSYPFRLEEYAMIGHSPYANECNKDEA
                     KFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELKTRLSTLHVNLEQFINSSEDD
                     EYTL"
BASE COUNT        13642 a        14869 c        13652 g        13605 t
ORIGIN      
        1 aagcactccc cttttgtgcg gtcagctttg cgtggcgttc gttttccgtc acaataagac
       61 ttttgccagg acattgttat gcaggccgaa atccttctca cccttaagct ccaacaaaaa
      121 ttattcgccg acccgcgccg catttcgcta ctaaaacaca ttgcgctttc cggttccatt
      181 agccagggag cgaaagatgc cggtatcagc tataaaagcg cctgggatgc cattaacgag
      241 atgaatcagt taagtgagca tattctggtc gagcgcgcaa caggcggtaa aggtggcgga
      301 ggcgcagtac tgacccgcta tggtcagcga ctgattcagc tctatgactt actggcgcaa
      361 atccagcaaa aagcctttga tgtgttaagt gatgatgacg ccctgccgct gaacagcctg
      421 ctggccgcga tctcacgttt ttcactgcaa accagcgccc gtaaccagtg gttcggtacc
      481 atcaccgccc gcgatcatga tgacgttcaa cagcatgttg atgtcttact ggctgacgga
      541 aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg cacgtctggg gctggatgaa
      601 ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag gcattactca ggacgaagcg
      661 gtcgcacaaa acgccgacaa ccaattaccg ggtattatta gtcatattga gcgcggcgca
      721 gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa cactgtgcgc cacagtgccg
      781 gtaaatgaag cgacttctct tcagcaaggg cagaatgtca cggcctactt taatgccgac
      841 agcgtgatta tcgccgcgct gtgctaagcg tgttgacaat ttgttatgaa acacgtatcc
      901 ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca tcgttgcaaa ttttgcaagg
      961 cacgtttcgt cttagcgaca caaaaacgct gcaattgcct cagctaacgt taaacgcggg
     1021 tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag tcggcgctgg cccgcgcgct
     1081 ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc cagttttccc acatcactcg
     1141 tctctccttc gagcaattgc aaaagctcgt cagcgacgaa tggcaacgga ataacaccga
     1201 tatgctcggc cctagcgaag atgacaccgg acgcactacg gctgagatta ttcaggatga
     1261 agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag ttcggtatta ccgccctcct
     1321 cgaccgacgc tttaaatacc tttccactgg cgagacgcga aaaaccctgc tgtgtcaggc
     1381 gctgatgtcg gagcctgact tgttgattct tgatgagccg ttcgatggcc tggatgttgc
     1441 ctcacgtcag cagctggctg agcgactcgc ctcgttacat cagtccggta ttactttggt
     1501 actggtgctc aatcgcttcg ataagatccc ggaatttgtc cagtttgctg gcgtgctggc
     1561 ggattgtacg ttagcggaaa ctggcgctaa agaggaactg ctccagcaag cactcgtcgc
     1621 gcaacttgcg catagtgaac agcttgaagg tgtgcaactg ccggagccgg atgaaccttc
     1681 agcacgtcac gccttacccg ccaacgaatc gcgcattgtg ctgaacaatg gcgtggtttc
     1741 ttataatgat cgccccattc ttaataacct tagctggcag gtgaatccag gcgaacactg
     1801 gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta ttaagcctga ttactggcga
     1861 tcatccgcaa ggttacagca acgatttgac gcttttcggc cgacgtcgcg gcagcagcga
     1921 aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc agtagtttgc atctggatta
     1981 ccgggtcagc actaccgtgc gtaatgtgat tctttctggc tattttgatt cgattggcat
     2041 ttatcaggcc gtttcggatc gccagcaaaa actggtgcag cagtggctgg atattctcgg
     2101 cattgataaa cacacggctg acgctccgtt ccatagtctt tcctggggac agcagcgtct
     2161 ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg cttattctcg atgaaccact
     2221 acaggggctt gatccgctca atcgccaact tatccgccgt tttgttgatg tgctgattag
     2281 cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct gaagatgcgc ctgcctgtat
     2341 tacccatcgc cttgagttcg tgccggacgg tggactctat cgctatgtgc tgacaaaaat
     2401 atattgagtc ggtagtgctg accttgccgg aggcggccta agcgccctct ccggccaacg
     2461 gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga taaaaagcgc aatcattcat
     2521 aaaccctctg ttttataatc acttaatcgc gcataaaaaa cggctaaatt cttgtgtaaa
     2581 cgattccact aatttattcc atgtcacact tttcgcatct ttgttatgct atggttattt
     2641 cataccataa gcctaatgga gcgaattatg agagttctgg ttaccggtgg tagcggttac
     2701 attggaagtc atacctgcgt gcaattactg caaaacggtc atgatgtcat cattcttgat
     2761 aacctctgta acagtaagcg cagcgtactg cctgttatcg agcgtttagg cggcaaacat
     2821 ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga tgaccgagat cctgcacgat
     2881 cacgctatcg acaccgtgat ccacttcgcc gggctgaaag ccgtcgccga atcggtacaa
     2941 aaaccgctgg aatattacga caacaatgtc aacggcactc tgcgcctgat tagcgccatg
     3001 cgcgccgcta acgtcaaaaa ctttattttt agctcctccg ccaccgttta tggcgatcag
     3061 ccccaaattc catacgttga aagcttcccg accggcacac cgcaaagccc ttatggcaaa
     3121 agcaaattga tggtggaaca gatcctcacc gacctgcaaa aagcccagcc ggactggagc
     3181 attgccctgc tgcgctactt caacccggtt ggcgcgcatc cgtcgggcga tatgggcgaa
     3241 gatccgcaag gcattccgaa taacctgatg ccatacatcg cccaagttgc tgtaggccgt
     3301 cgcgactcgc tggcgatttt tggtaacgat tatccgaccg aagatggtac tggcgtacgc
     3361 gattacatcc acgtaatgga tctggcggac ggtcacgtcg tggcgatgga aaaactggcg
     3421 aacaagccag gcgtacacat ctacaacctc ggtgctggcg taggcagcag cgtgctggac
     3481 gtggttaatg ccttcagcaa agcctgcggc aaaccggtta actatcattt tgcaccgcgt
     3541 cgcgaaggcg accttccggc ctactgggcg gacgccagca aagccgaccg tgaactgaac
     3601 tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca cctggcactg gcagtcacgc
     3661 catccacagg gatatcccga ttaaggaacg accatgacgc aatttaatcc cgttgatcat
     3721 ccacatcgcc gctacaaccc gctcaccggg caatggattc tggtttcacc gcaccgcgct
     3781 aagcgcccct ggcagggggc gcaggaaacg ccagccaaac aggtgttacc tgcgcacgat
     3841 ccagattgct tcctctgcgc aggtaatgtg cgggtgacag gcgataaaaa ccccgattac
     3901 accgggactt acgttttcac taatgacttt gcggccttga tgtctgacac gccagatgcg
     3961 ccagaaagca acgatccttt gatgcgttgc cagagcgcgc gcggtaccag ccgggtgatc
     4021 tgcttttcac cggatcacag taaaacgttg ccagaactga gcgttgcggc attgacggaa
     4081 atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa aaacgtaccc gtgggtgcag
     4141 gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc cgcatccgca cggacagatt
     4201 tgggcaaata gcttcctgcc taacgaaact gagcgcgaag accgcctgca aaaagaatat
     4261 tttgccgaac agaaatcacc aatgctggtg gattatgttc agcgcgagct ggcagacggt
     4321 agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg tgccttactg ggctgcctgg
     4381 ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc ggatcaccga tttgaccgac
     4441 gcccagcgca gcgatttggc gctggcgttg aaaaagctga ccagtcgtta tgacaacctc
     4501 ttccagtgct ccttccccta ctctatgggc tggcacggcg caccatttaa tggcgaagag
     4561 aatcaacact ggcagctgca cgcgcacttt tatccgcctc tgctgcgctc cgccaccgta
     4621 cgtaaattta tggttggtta tgaaatgctg gcagagaccc agcgagacct gaccgcagaa
     4681 caggcagcag agcgtttgcg cgcagtcagc gatatccatt ttcgcgaatc cggagtgtaa
     4741 gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca acgcatttgg ctaccctgcc
     4801 actcacacca ttcaggcgcc tggccgcgtg aatttgattg gtgaacacac cgactacaac
     4861 gacggtttcg ttctgccctg cgcgattgat tatcaaaccg tgatcagctg tgcaccacgc
     4921 gatgaccgta aagttcgcgt gatggcagcc gattatgaaa atcagctcga cgagttttcc
     4981 ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg cgaactacgt tcgtggcgtg
     5041 gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg tggacatggt gatcagcggc
     5101 aatgtgccgc agggtgccgg gttaagttct tccgcttcac tggaagtcgc ggtcggaacc
     5161 gtattgcagc agctttatca tctgccgctg gacggcgcac aaatcgcgct taacggtcag
     5221 gaagcagaaa accagtttgt tggctgtaac tgcgggatca tggatcagct aatttccgca
     5281 ctcggcaaga aagatcatgc cttgctgatt gactgccgct cactggggac taaagcagtt
     5341 tccatgccga aaggtgtggc tgtcgtcatc atcaacagta acttcaaacg taccctggtt
     5401 ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg gtgcgcgttt cttccagcag
     5461 ccagctctgc gcgatgtcac cattgaagag ttcaacgctg ttgcacatga gctggaccca
     5521 atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg cccgcaccgt tgaagctgcc
     5581 agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt tgatggcgga gtctcatgcc
     5641 tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg acactctggt agaaatcgtc
     5701 aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg gcggcggatt tggcggctgt
     5761 atcgtcgcgc tgatcccgga agagctggtg cctgccgtac agcaagctgt cgctgaacaa
     5821 tatgaagcaa aaacaggtat taaagagact ttttacgttt gtaaaccatc acaaggagca
     5881 ggacagtgct gaacgaaact cccgcactgg cacccgatgg tcagccgtac cgactgttaa
     5941 ctttgcgtaa caacgcaggg atggtagtca cgctgatgga ctggggtgcg actttacttt
     6001 ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc gctgctcggc tgtgccagtc
     6061 cggaatgcta tcaggatcag gctgcgtttc tgggggcctc tattggtcgt tataccaacc
     6121 gcatcgccaa tagtcgttat acctttgacg gtgaaaccgt gacgctttcg ccaagccagg
     6181 gcgttaacca gctgcacggc ggcccggaag ggttcgacaa acgtcgctgg cagattgtga
     6241 accagaacga tcgtcaggtg ctgtttgccc tgagttcaga tgatggcgat cagggcttcc
     6301 cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga cgataaccgt atctccatta
     6361 cttatcgcgc cacagttgat aaaccttgcc cggtgaatat gactaatcac gtctatttca
     6421 atcttgacgg cgagcagtct gacgtgcgca atcacaagtt gcagattctg gcggacgaat
     6481 atcttccggt tgatgaaggc ggcattccgc acgacggctt gaaatctgtc gccggaacgt
     6541 cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt tcttgccgac gacgatcagc
     6601 gcaaagtgaa aggttacgat cacgcattct tgttacaggc caaaggcgat ggcaagaaag
     6661 tggcggcgca tgtctggtca gcagatgaaa aattgcagct gaaggtctac accaccgctc
     6721 cggctcttca attctactcc ggcaacttcc tcggcggcac accgtcgcgg ggaaccgaac
     6781 cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt tctgccggac agcccgaacc
     6841 accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg cgaagagtat tccagcctga
     6901 cggaatatca gtttattgct gagtaatgtt aagccctcgt catgagggct ttatcttcat
     6961 attgttcaaa tcaccagcaa acaccgacat atttgcaact caatattcac aacaacctta
     7021 cactgcgcca ctattttcgc tatggttatg cgtaagcatt gctgttgctt cgtcacggca
     7081 atataatgag aattattatc attaaaagat gatttgagga gtaagtatat ggctgtaact
     7141 aagctggttc tggttcgtca tggcgaaagt cagtggaaca aagaaaaccg tttcaccggt
     7201 tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag caaaagcagc aggtaagctg
     7261 ctgaaagagg aaggttacag ctttgacttt gcttacactt ctgtgctgaa acgcgctatc
     7321 cataccctgt ggaatgtgct ggacgaactg gatcaggcat ggctgcccgt tgagaaatcc
     7381 tggaaactga acgaacgtca ctacggtgcg ttgcagggtc tgaacaaagc ggaaactgct
     7441 gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg gttttgcagt gactccgccg
     7501 gaactgacta aagatgatga gcgttatccg ggtcacgatc cgcgttacgc gaaactgagc
     7561 gagaaagaat tgccgctgac ggaaagcctg gcgctgacca ttgaccgcgt gatcccttac
     7621 tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc gcgtaatcat cgccgcacac
     7681 ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca tgagcgaaga agagattctt
     7741 gagctgaata tcccgactgg cgtgccgctg gtgtatgagt tcgacgagaa tttcaaaccg
     7801 ctgaaacgct attatctggg taatgctgac gagatcgcgg cgaaagcagc agccgttgcg
     7861 aaccagggta aagcgaagta aacgtcattc gtttaaaaaa tgagaaagcc gactgcaagt
     7921 gagtcggctt ttttgtttgt cgttgctaac aatggagcac attgcctgat gcgacgctgc
     7981 gcgtcttatc aggcctgtgg tgattcatcg gatacgccac tctgacggcg catccgacaa
     8041 ttaaacctta cccgcgacgc gcttttactg cattcgccag ttgacgtaac agagcatcgg
     8101 tatcttccca gccaatgcag gcatcggtga tgctcttacc gtaggccagc ggctccccgc
     8161 tatcgaggct ctgattgcct tccaccagat ggctttccac catcacgcca ataatggcct
     8221 tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac atccatctgc tttttgaatt
     8281 gtttggacga attagcatgg ctgaaatcga tcatcacctg agctggcaga cctgctttgt
     8341 tcagcccttc tttcacttca gcaacgtgct tcgcgctgta gttaggctct ttaccgccgc
     8401 gcagaatgat atggcaatcg ccgttaccac tggtattcac aatcgccgaa tgcccccatt
     8461 tagttacgga caggaagcag tgcggcgcac cggcggcatt aatggcatcg atagccactt
     8521 taatcgtacc gtctgtgcca tttttaaaac ctaccggaca agaaagacca gacgccagtt
     8581 cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc gccccagctc atcaggtcag
     8641 cgagatactg aggagtgatc atatcgagaa actcacccgc cgctggcaga ccgctgtcgt
     8701 taatatcaag cagcaattta cgggctatac gcagaccgtc gttgatctgg aagctgttat
     8761 ccatatgcgg atcgttaatc aaccctttcc agcccaccgt ggtacgcggc ttttcaaaat
     8821 agacgcgcat tacgatttcc agctcatctt tcagctcttc acgcagcgcc agcaagcgag
     8881 tggcatactc tttagccgcg acaggatcat gaattgagca cgggccaatc accaccaaca
     8941 ggcgatcatc attacctttc aggatcttat ggatcgcttt tcgggcatga gcaaccgtat
     9001 tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc gacaggagga agtaactctt
     9061 tgatttcttt gatgcgtaaa tcgtcgttct gataattcat gtctgttcca gtgtcgccat
     9121 acttatctta gtgaatgcaa tacttccaat ctatatcttc cgtcagaatg tgtaaacggg
     9181 gttttacact atgaacggat taatcctaga attagcaaga aaaacgccag attgtcgcga
     9241 aaaacgagat ctttcctaca atttctaact gtaactcctt tgtttgttaa ttatttcaag
     9301 attctctgct gcgtttcata aactggctga aatcttaaac caatgcctta tattcacctg
     9361 caaatgcact gttggaagag gttatccgac ataacgacca taacaggagc atcctatgaa
     9421 aatgacaaaa ctggccacat tatttctgac tgccactcta agccttgcca gcggtgccgc
     9481 actggctgcc gatagcggag cgcaaactaa taacggccag gcaaacgccg cagctgatgc
     9541 gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca aataacgtcg acaataacgg
     9601 ggtaaatacc ggttctggcg gcacaatgct gcattcggat ggttcttcaa tgaacaatga
     9661 cggaatgacc aaagatgaag agcacaagaa caccatgtgc aaagatggtc gatgtccgga
     9721 tatcaataaa aaggtacaaa ccggtgatgg catcaataat gatgtcgata ccaaaaccga
     9781 cggtaccacg cagtaaccct gcaaaatagc caataatggg agagcggagt ctctcccttt
     9841 taatttttca cttctaaaag cagtatgatg tcataaagtt aagtgagaat aattaaggga
     9901 tgactcgtca tggcgcactc acactcacac acgtcctcac acctgccaga agataataat
     9961 gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt ttatgctggt agaagtcgtt
    10021 ggtggttttc tttctggttc tctggcattg ctggccgatg cgggtcatat gttgaccgat
    10081 actgccgccc tgcttttcgc cctactcgcc gtgcaatttt cacgtcgtcc tcccaccatt
    10141 cgccacactt tcggttggct aagactgacc acgctcgcgg cgtttgtgaa tgccatcgcc
    10201 ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag aacgcttccg cacgccgcgt
    10261 ccggtcgagg gaggcatgat gatggcaatt gccgtggctg ggttgctggc aaatatactt
    10321 tctttctggt tacttcatca cggcagcgaa gagaaaaacc tcaacgtacg agcagcggca
    10381 ctgcatgtgc tcggggacct gctgggttcg gttggggcta ttattgccgc gttgattatt
    10441 atctggactg gctggacacc tgctgacccc attctctcga tactggtgtc gcttctggtt
    10501 ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg aattacttga aggtgcaccg
    10561 gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc gggaaatccc ggaagtccgc
    10621 aatgtgcacc atgtacatgt atggatggta ggcgagaagc cggtgatgac gttgcatgtg
    10681 caggtgatcc cgccacacga tcacgatgcc ttgttggatc agatccaaca ctacctgatg
    10741 gatcactatc agattgagca cgccaccatt cagatggaat atcaaccttg tcatgggcca
    10801 gactgccacc tgaatgaagg ggtgtctggc cattcacatc atcaccatta atgggacagc
    10861 gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc ggctgccgtt gagcgcaata
    10921 aaggtcagga tgatgtactc cagagacatg gcgtaaacgc cctgaagtgc aaaaataacg
    10981 acgctaatca cgttaataat cacccacaac agccagtttt ccacatactt acgcgtcatc
    11041 agaatcattg ccacgataga taacaccatc atgcatgaat cccagaacgg gaaagcgtcc
    11101 ggttgcagtt caggcatcac aacctgtaat cctaatgctt gcatgatcat gactgccacg
    11161 cgggtcaaaa atgcaaacac cggattgata aataccgtca tcagaccaat cgaaacaacg
    11221 caaaccgcca accagctgag tgccttcggc aatggcaacc agcgaatttt caactccgcc
    11281 tcgttctgac tggtttgtcg cgaccacgca taccagccgt aaatattcgc ggcaaagaaa
    11341 aacacctgta atagcaggct ggcatacagc tgaatctgaa agaaaataat gccaaacaag
    11401 gtaacgttga tcaggccaaa gaagtagttg ctgatcttct ccagactggc aaggccaata
    11461 cacagcaacc cggcgatcgt gcctaccgct tcgatccatg agagatcata accgcccgcc
    11521 cctattggta tatgtaccag gatattctgc acactaaaaa aatccatctt tcccccaaag
    11581 cgtaacgtta cgccttatta ttcgttatcc acgtagtgta accgcaaaat ccagcatacg
    11641 attgagcggc accagcgccc tctctcgcag cctctcatcg acatgaacct cgtgattact
    11701 tccttcctgt tctaatgcct ctgcgatagc ctgaaggcca ttcatggcca tccacggaca
    11761 atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt ggtgcttcca gtaactcttt
    11821 atctggcacc gcctgctgca ttttgtagaa aataccccga tcggttgcca caataagcct
    11881 ctgatgaggc aatgcttttg cagcagcgat cagttgactg gtggaaccga ccgcatccgc
    11941 catatcgaca atagcttgtg gtgattctgg atgcaccagt atggcagcat ccgggtattc
    12001 ttcttgcaag cgggttaacg cctgagtctt aaattcatca tgcacaatgc aggcaccctg
    12061 ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa cgccccagat gtttgtcggg
    12121 tgcccagatg attttttcac ccaaactatc aagatgatca ataagttcga cggcaatgct
    12181 tgaagttacc acccaatctg cgcgcgtttt taccgcagca gaagtgttgg cgtagacgac
    12241 gacagtacga tcgggatggg catcgcaaaa tgcgttaaat tcttcaacag ggcagccgag
    12301 atccagtgaa cactcaacct gaagtgtcgg catcagaatt gttttttccg gactgagaat
    12361 tttggcggtt tctcccataa atctcacccc agcgactaac aaagtagaag cgggatgctt
    12421 tgcaccgaag cgcgccattt ccagagaatc agaaatacag ccaccggttt cttctgccag
    12481 ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc accgcattac gttcttttag
    12541 cagacgtttt atcttctcgc ggtaatacgc tttttcatca atgcttaacg gcgtcggctt
    12601 cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt acgctcatct taccatctcg
    12661 ttttacaggc ttaacgttaa aaccgacatt agcgtaacat tcgcttttat cggtgtaatt
    12721 tgttttatat gctaaacaag atagtcgaat ggtggggaaa agtcactcga attacgccta
    12781 attccaccga cgtagagttg cttgcacagt aagctctgac gatgtcatca ggtttacaaa
    12841 gtgaaattga ggatgcctga atagcaaaaa agcgccttta gggcgctttt ttacattggt
    12901 gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg
    12961 agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag
    13021 ggaggtgctc tcccagctga gctaatcacc cgatactgcg ctggatacta caaactactt
    13081 ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat tcgaacctgc gaccaattga
    13141 ttaaaagtca actgctctac caactgagct aacgacccgg tggtgggtga tgacgggatc
    13201 gaaccgccga ccccctcctt gtaagggagg tgctctccca gctgagctaa tcacccgaca
    13261 ctacgctgga tactacaact aactatgtta gtggtgggtc gtgcaggatg actcgacttc
    13321 gtctcgccct tcggcgccgt tgctgatgca acgttatcct tcacatttta ctaccgcttc
    13381 caccttagcg attggtgggt cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca
    13441 actgctctac caactgagct aacgacccac tttttcgttg ctttcggttt gtttgatatc
    13501 ccgtggcaac ggcggcatat attactgatt tcagacttga gcgcaacaaa aatttcgatg
    13561 cagatcactc aactgcttat gattcgcacg acacgaccag aaataatgcg acttctggtc
    13621 gtgtgttatg cattacatcg cgttcagacg tttttgtgcc tgtttagcgc catcagtacc
    13681 agggtattta ctgataacct gctggtatac ggctttcgct tttgcggtgt cacctttgtc
    13741 ctgcatgatg acgccgactt taaacatcgc atcagcagcc tttggtgact tcggatagtt
    13801 tttcactacc gaagcaaaat agtacgccgc atcatctttt ttacccttgt tgtagtttaa
    13861 ctgacccagc caataattgg cgtttggcag gtaagttgaa tcagggtaat ttttgatgaa
    13921 attctgaaat gccaccattg cgtcatcctg gcgggattta tcctgcacca gcgcaatagc
    13981 tgcattgtaa tccgtgtttg catcgccgct tttcaccggc gcgccagcat tcgcagtacc
    14041 agcatcagct gtcggcgtcg ttgatgccgc cgcaccgctt tgatcgctgc tggttgattg
    14101 cgccgctgca ccaccgctgc tgaggctgtc gatctgcaac aggatctgct tttgccgctc
    14161 cacgacctga ttcagttgat actgattttc ctgaatctga ccacgcaggg aatcaatatc
    14221 ggattgatta tcagaaagtt gttgctggag ttgggttaaa agctggctgt gagcattaga
    14281 aatacgctca agttgagtga cgcggtcttc gaccgagcct gagccgacac tactgattgg
    14341 tgcctgagca aaagcggccc agggggccgc tataccaacc agtaacgaca gactcaatag
    14401 ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa accagtaccg cacgacggtt
    14461 tttggagtat gctgcttcgt catgacccag tactgcaggt ttttctttac cgtaagaaac
    14521 gatggagatc tggtctgcag aaacgccttt accctgcagg tacatcttaa cggcgttcgc
    14581 acgacgttca cccagggaga tgttgtattc cggagtacca cgttcgtccg cgtgaccttc
    14641 tacggtgact ttgtaagacg ggttgctacg caggaagttt gcatgtgcat ccagcatttg
    14701 agcgaagtca gaacggatat cgtacttgtc cagatcgaag taaacgatgt tgttctgctg
    14761 cagctgttgc atttgcagac gagcctgctc ttcggaagac atgttgccgt tgccgccgtt
    14821 cgcatccata ccagtgccag cacccagcat gccttcgctg ccgtcattgc tggcgttctt
    14881 gttggaagaa catgccgcaa ttgccataac aggcagagca atcatcagcc ctttcagcac
    14941 tttgttcagt tgcatttcaa tgattccttt actattcaat taattattat cacagatacg
    15001 gcgaccaggc agggaatttg acctgtccat cagttgccgg aagacgcgct ttgaaacgcc
    15061 catctgtaga aaccaaattc agcacggatc ccatcccctg agaagagctg tagattacca
    15121 tagtgccgtt aggtgccaga cttggcgttt catccaggaa cgtggacgac agaacttgta
    15181 cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg cccaccattg gagctgacca
    15241 ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg gttctgcgaa ccttcccagg
    15301 taatacgttg tggcgcaccg ccgttgatat tcactttata aacctgcgga cgaccggcct
    15361 ggtcagaagt aaatgccagg ttctggctat ccgggaacca ggtcggttcg gtattgttac
    15421 tgcgaccatc agtcacctgg cggatctgac cagaagccaa atccattacg tacaggttca
    15481 gactaccggt tttcgacaag gcgaatgcca gtttgctgcc gtctggcgag aatgcaggtg
    15541 caccgttgtg acgcgggaat gaagccacct gacgtacagc gccatttgcc agcgtctgaa
    15601 taaccagcgc ggaacgaccg ctttcgaagg tcacataagc cagtttagaa ccgtctggtg
    15661 accacgccgg agacatcagc ggctgcggtg aacggtgaac gacaaactgg ttgtaaccgt
    15721 catagtcaga tacgcgcagt tcatacggga actgaccgcc gttggtctga acaacgtagg
    15781 caatacgggt acggaacgca cctttaatgc cggtcagttt ttcaaacact tcatcactgg
    15841 cggtatgacc agcataacgc agccactgct tgttcacttt gtacgagttc tgagcaagta
    15901 cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc aacattgtaa gaaccatccg
    15961 gattcggagt gacctgaccg acaactacag cgtcaatgcc cagtgcggac catgcagctg
    16021 gttgtacttc ctgcgcacta cccggctgct gtggcagacg agcgcgatct aacggattaa
    16081 atttaccgct gttacgcaag tcagcagcaa cgatgccgcc aatatcttca ggtgccgcac
    16141 caggccccgc ccactggaaa ggaacaacac caataggacg accggaatct acaccgctgt
    16201 cgatcacaat gcggacttca gcatgcagaa ctgatgccca cagtatgaga aaaccaaatg
    16261 ctactcgtaa tgcctgcttc atcatatctc ccttatctgg accgatggcc cacgataatt
    16321 tagcagaatg ttaacaaact aaaatacaca aaactaccag aaccccgtgg caaccggtgc
    16381 ctgatgttga ccgtccgaac agtcaacatc gcgattacgg tttgaagtcc aatggcgcgt
    16441 ttttgaacac ttcatatact gcctggcttg gtggtttcgg gatcttcgca agtttagctg
    16501 ctgccaacgc agcctgacaa agtgcgggat cgccaccttc aggtttgata tccagtaaca
    16561 taccatcggg tgccagtttt atgcgcagcg tacaggtttt gcctgcatag gacgatgcgt
    16621 catagaactt actttcgata gcagatttaa tctgcccggc atagttattg atatcggccc
    16681 ctgatgcgcc attgttttta gtattaccac tcccggcagg cgaagcattg ttccctttcg
    16741 cccctccccc cgttttcggt gcattcttac cagagcttag ctcaccgaaa atatcatctg
    16801 cctctgcggc agcttttgct gcagcggctt tttcagctgc tgcttttgcc gctgccgctt
    16861 ttttgtctgc ggcggctttt tctgccgctg ctttcttatc agctgctgcc ttatcagcag
    16921 ccgctttttc tgctgctttt ttgtcggctg cagctttctc tgctgccgct ttcttatcag
    16981 ctgcagcctt ttcagcagcc gctttcttct cagcttctgc cttggctttt tcagcagctt
    17041 cagttgccgc tttctttctt gcttcagctg cagctgcttc tgccgcttcc gctttcttct
    17101 tcagtgccgc agcggctacc tcggcttttt tctgcgcttc ggctgcggct ttggcggctt
    17161 ctgcttctgc ttttttcttc gcgtctgcag ccgctttctt cgcagcttct tccgcagctt
    17221 tagcatctgc cgccgctttc gctgccgcca cttcagcttg cttctgcttt aactcggcct
    17281 gttttgcggc ttcttcagcc tgctttttct gctcctgagc cgctaaccgc tctttctcaa
    17341 gttgcttcag gcgttcctgt tcagccgctt gtttctcacg cagttcttca gcagcctgct
    17401 gttccttcat cttgcgctgt tcatcagaac gcttcgcgct tgattcctgg ctttgcatgc
    17461 gtttgtactg ctcaactacc gcacctgaat caaccatgac agcgtcgatg gacgaaccac
    17521 cgccgcctcc ggctgaagct tctatattct catcgaacga actccagatc agcgccgcaa
    17581 ataagatgac atgcagcact gctgaaataa ttatcgcccg cttgagcttg tcgttttgtt
    17641 cggttgcctt tgacactctc ggtttccaaa aactgttcgc ctgttacccg ctctctttca
    17701 agcaagggaa acgcagatgt ttagataggc tgcgtcatta aaccaaccga tttcacaccc
    17761 gcactatgta acaagttcag tgctttaatt atttcatcgt aaggcacatc ttttgcgcca
    17821 ccgatcagaa agaccgtttt cgggttggcc ttgaaacggc tggacacttc cgccaccacc
    17881 tgctctggtg gtaaacgctc caggcgatct ttctcaacca ccacggtgta ctgaccaata
    17941 ccagacactt caacaatcac tggcggatta tcgttactgc tcaccgcctg tgattcagta
    18001 gcgtctggca gatcgacctc cacgctctgg gtgatgatgg gcgctgtcgc cataaagatc
    18061 agcaacagca ccagcagtac gtccagcaac ggtacaatgt tgatttcgga cttgagatcg
    18121 cgacgacctc gtccacgcgc tctggccatg gcttacccct tgttgctctc gctaacggta
    18181 aacgcctggc ggtgcagaat cgcggtaaac tcttccataa agttgtcgta attcagttcc
    18241 agtttgttta cgcgctggtt gagacggtta taagccatta ccgccggaat tgcggcgaac
    18301 agaccaattg ccgtcgcaat caacgcttct gcgataccgg gcgcaaccat ttgcagtgtt
    18361 gcttgtttta ccgccccgag ggcgataaag gcgtgcatga tcccccagac cgtaccaaac
    18421 agaccaatat acgggctgat ggagccaacc gtaccgagga acggaatgtg cgtttccaga
    18481 ttttcaagtt cacggttcat ggagatacgc atagcacgcg acgccccttc cactacggct
    18541 tccggcgcat ggctattggc acgatgcagg cgcacaaact ctttgaaccc gctgtaaaag
    18601 atttgttccg aaccagtcag attatcccgt ttcccctgac tctcttgata gaggcgagag
    18661 agttcgattc cagaccagaa tttatcttca aacgcttcgg cttcacgcgc cgctgcgtta
    18721 agaatacggg ttcgctggat aataatggcc caggatgcga ttgaaaaacc aatcaaaatc
    18781 aacatgataa gtttaaccag aaggctagcc ttcaggaaca aatcaaggat attcatgtca
    18841 gtcactgctt aaactccgcg acaatagact tgggaagcgc acgaggcttc attttgagtg
    18901 ggtcaacgca aacaaccaga acctctgctt cattcagcaa agtattctcg gcgttgacaa
    18961 tatgttgcgt gaaaaccaaa gaggtgccac gcattgatgt tatttcagtc tgtatttcga
    19021 gcatatcgtc gagccgcgca ggtgcgtaat attccaccgt cattttacgt accacaaagg
    19081 caacgcgttc agccatcagc gcctgctgac tgaagtgatg atgacgcagc atctctgtgc
    19141 gtgctctttc ataaaaagcg acgtaactgg cgtggtacac cacaccaccg gcatcggtat
    19201 cttcatagta gacgcgaacc ggccatcgaa acagcgttgt attcacttta catcccggta
    19261 atgcaacaaa agttagagct tttaaacgct gctactatac gcgcgcgatt gaggtttggg
    19321 aatgagggca agttaaggga gcgtaaaaat ttatgggcct ggagcggccc ataatttttt
    19381 aaagagaatt ctgatttaaa agaagaagaa aatcagcccg acaatgagaa caatatcggc
    19441 aagcaatggg caaaaaatcc cttgccaaag aaccttctgc ggacgaaagc ccacgccgtg
    19501 aatcacacca gcacagacgg cccacatcag caataaacca tgccagattt ccagttcact
    19561 ggtcttcgcg gcaaaacgag atgggtccca aaacatacat cctgctaaca gaagcgccat
    19621 cacgaaggaa agcgcccgca gggggcgctt gtccattacc gcatacaagg tcgcgataat
    19681 cttactcatc agatgtcttc ttgaccggct ttgcctgatt cgacgtgttc aagcgccagc
    19741 gcggtgatta ccccaaacga acaggcaaga agcgttccca gaatccatgc gaaataccac
    19801 atttttagct ccttacttag tacagagagt gggtgttacg ttcaatatct tctttggtga
    19861 tacgaccgaa cattttccag taacaccagg cggtgtagag cagaatgatc ggtaccagaa
    19921 ccaccgcaac ccaggtcatg acgttaagcg tcagctggct ggaagttgca tcccacattg
    19981 tcagacttgc gttcatcatg gtgctggacg gcatcacaaa cgggaacatt gcgataccgg
    20041 ctgtcaggat gatgcaggcc agcgtcaggg aggagaacac aaacgcccac gcggctttat
    20101 ccatacgtgc agtcaggatg gtcagcagcg gcagaaccac acccagtgcc ggaatagccc
    20161 acagaattgg cgtgttgttg aagttaacca gccatgcgcc agcttcacga accacttctt
    20221 tattcagtgg gttagaggct gcgtaatggt ccattgtcga tttcacgaca taaccatcga
    20281 taccgtacat cacccatacg ccagccagtg cgaaacagac cagtgtcacc agcgcagcca
    20341 cctgagccgt tgcacgggta cgcaggtgca gttcgccccc ggtacgcatt tgcagatagg
    20401 ttgcgccctg agtaatgatc atccctacgc tcaccacgcc tgccagcagg ccgaacgggt
    20461 taagcaactg gaagaagtta ccggtgtagt acagacgcag atattcatca acgttgaacg
    20521 gtacgccctg caacaggtta ccgaacgcta caccaattac cagcggcgga acgaagctac
    20581 caatgaagat gccccagtcc cacatgttac gccagcgggt ttcttcaatc ttggagcggt
    20641 agtcaaaacc gaccggacgg aagaacaaag acgccagcac gaggatcatc gccacataga
    20701 agccggagaa cgcagcggca tagaccatcg gccaggcagc aaagagtgcg ccgcccgcgg
    20761 tgatcagcca aacctggtta ccgtcccagt gtggtgcaat ggagttaatc ataattcgac
    20821 gctcggtgtc gttacgaccg aggaaacggg tgagcatgcc cacccccatg tcgaaaccgt
    20881 cagtgactgc aaaaccaatc agcagaacgc caaccagcag ccaccagata aaacgcaata
    20941 cttcataatc gatcatttga cgactcctgt cttagcgtgc cggctgagta gtcgtggaag
    21001 actgctcaaa gtgatagcga ccggttttca ggctgcttgg gccgaggcgt gcaaacttga
    21061 acattaagaa caattctgcc accaggaaca gggtatacag gccgcaaatc agcaccattg
    21121 agaagatgag atcgcctgcg gtcagtgacg aattcgccac agctgtcggc agcacttcac
    21181 cgatagccca cggttggcgg ccatattcag ccacgaacca gcccgcttct acagcaatcc
    21241 acggcagcgg aataccgtac agcgcggcgc gcagaagcca ttttttctcg ccaatgcggt
    21301 tgcggatgac actccagaaa gagagcgcga tgattgccag cagcaggaag ccacacgcca
    21361 ccatgatacg gaacgcaaag tacagcggcg ctacacgcgg gatggagtct ttggttgcct
    21421 gttgaatctg cgcttcagtc gcatcagcca cgtttggcgt atagcgtttc agcagcagac
    21481 cgtaaccgag gtctttcttc atgctattga actggtcacg aaccgcctgg tcggtagaac
    21541 cagaacgcag ttgttcgagc agagagtacg ccttcatccc gttacgaatg cgttcttcat
    21601 gctgcaccat cagctctttc aggccgataa ccggggtatc cacggaacgc gttgcaatga
    21661 tgcccagtgc gtaagggatc tgaatcgcaa atttgttcgt ctcctcttcc tgatcaggaa
    21721 tgccgaacaa agtaaaggca gcaggcgcag gttgcgtttc ccactcggct tcaatagcag
    21781 ccagtttggt tttctgcacg tcgcccattt cgtagccgga ttcatcaccc agaacaataa
    21841 cagacagaac agcagccata ccgaagctgg cagcgatagc aaaggagcgt ttagcgaagg
    21901 cgaagtcacg acctttcagc atataccatg cgctgatacc gaggatgaac atcgcgccag
    21961 tcacataacc agacgctaca gtgtgaacga atttcacctg agcaaccggg ttaagcacca
    22021 gctcggagaa gctcaccatc tccatacgca tagtttcaaa gttgaaatcg gacgcgattg
    22081 ggttttgcat ccagccgttc gcaatcagaa tccacagtgc ggacaggttt gaaccgagcg
    22141 ccaccagcca ggtgacacac atatgctgaa ctttacccag acgatcccaa ccgaagaaga
    22201 acagacctac aaaggtggat tcgaggaaga aggccatcag accttcgatt gccagcggcg
    22261 caccgaagat atcccctaca tagtgggaat agtaagacca gttagtcccg aactggaact
    22321 ccatggtcag accggtagcc acacccagag cgaagttgat accaaacaac ttgccccaga
    22381 acttggtcat atctttataa atctgtttgc cggagaggac gtagaccgtt tccataatgg
    22441 ccaggaggaa cgccataccg agcgtcagtg gcacaaaaag gaagtggtac atcgcggtca
    22501 aggcaaactg taagcgcgac agttcgacta tatctaacat catgactcct tgctcatcgc
    22561 atgaagactc cgagagtgaa ccccgttaga aagggtcaca cgcatgcccc aatagaaatt
    22621 tgtttgaaca ctttcatcat accatgtgaa tgcacacaaa aacggcgtaa agaaaggttg
    22681 caaacatgtt aataaaaact caaattgatc ccacgtatat attacgccgc aaaatcctta
    22741 caataaacag gtttttgttg aagcaaattt gcatttttcg acggtgatca atttatagct
    22801 aaattgccgc ctttcagcca atttgatcga gaacaattta tctctttttg atgcccattt
    22861 ccaagactta tacattgata aatatcaatt tctacaaatt atgttaatca tgtttcaaca
    22921 aggtaacttt agtgacattt atgtttaaaa tgtgtgaatt ataagactga aattagtttc
    22981 ggttggcaat taaagataaa aagagacgaa gcaaaaacag cacggttgac cttaattctt
    23041 tttttggtca ctggctaata cgacgcagag tttcaggatt attaataaag gcataaatct
    23101 cctccccgcc gatattaaca tttaattaac ttataattat attataatta actcacccgg
    23161 ggttattatt ttgttaaaaa taaagccaca tatacgtagc ttgagagtga agataaacgc
    23221 aaaattacct gatgcgctac gcttgccagt gtctacatca tctctgcaat atattgaatt
    23281 tgcctgcttt tgtagaccga ataaagcgtt catgccgtat ccggcatgaa caaagcgccc
    23341 tttgtcaaca atctggccgc gcatatgtgg cttcggtttg attgctattc aggcaagccg
    23401 gtaactgaac gtccgtgact ggccgggtaa aaaaggcccc gatagatttg aaccttgatt
    23461 ttcctcggtg gtaatgtgtt catccatcat cgtttctgag caagaaatca cctcgcgact
    23521 gaaagcaaca gtcgcatcac aggttgctga ttcagccgga ttaaacagcc gtaaaatcac
    23581 ttcttgtcgg tcttcagctt tcttaagtgc gcttatcagg catcccactg ggggcatttt
    23641 caacaaacta taactttccg gcacgttgaa tccggctttg ttgagcttca tcgcatccca
    23701 tgggattttg ttgtaacact gtactggagt cagccatgct cgcgcctgct gagctacacc
    23761 agcggcggtt ggcgtaccgg tataactcaa taaacttagg cgacaagaaa gcagaccacg
    23821 tagttgtgag tccgggactg gcattttaat tcccgaaggc cgcctaggcc ttaaaagcag
    23881 atcttctttc cccagtaagc ccacgccgcg cagcaacgta atggcaaagg ttttcttctc
    23941 ttcaccgatg acttcaaatt cacgtagccc ttcgctaaag acagccatgc cgttacgccc
    24001 ttcctgtaag gctgcatagt tgagcatatt ccataccgga accggcgctt ctttccagcc
    24061 ttcttgctgc cagttgttca ttgcactgtc gttcacgggg cgcgttagcg aaccaaactg
    24121 ggtatctgcc agaacactat cggtgttaaa agatgtaggg atcaggacgc gaaggcgatg
    24181 atcgtcagcc tggttatcaa ggttgatatc cacatcaatg cgcctgttgt tatgactaag
    24241 agtgacaacc aactcgacac ctactctgcc agtggattgt cttgcgctgc gttgttgcag
    24301 attacgcggc accgccattt catagcggat aacagccctg ctctgccagg cctcatgaat
    24361 aatctcgcat tgcggtttcg cattcgccga agtaattacc cactcttctt ttgcgggtga
    24421 atagtcatat tcatcaccat catcagagct ttcttcaatt tgcaacaccc ggtcatagcg
    24481 cacaccgcta tctttatcta ccagttgtag cgttccatcc tcattgagcg caatttgcca
    24541 gaaagcattt tccagtatcc cttcagcgtc actttttgcc gcaatcacgt tgccaggctg
    24601 attcgcttcg atataaagcg tgcgatagcc catagaaggg acaatctggt tgatctgtat
    24661 atcaaactcc ataaagggat cgtaattacc gtaatgaact atttgccgat cgattaggcc
    24721 tggatcgatc tcacgggcat ggcgaataaa atacggtaca ggctgaccgc gatcgtctcg
    24781 tagattaaac tggctggcgc gcagccgcac agtggtgttg atcacttctt cccgcggcca
    24841 gggcatcagg ttaaacagga cgagtttgtc ggcgtcgctc tgcggcatgt tgtcggcgat
    24901 tttacgcata tagaaacgca gcagattatc cgccatgtct tcagccagtt cgaagcgggc
    24961 gacgatttcg cgatgaactt tgtcactaca gcagcagccg atgctgtcgt gggcatgatt
    25021 ttttaagatc tctttccata ttttctccag caagccgtgg tggtattcaa aacccaacgt
    25081 ccaggccagc gttgccagcg gttccagcgg ttccagcggt tccagcggtt ccagcggttc
    25141 cagcggttcc agcggttcca gcggttccag cggttccagc agattaacaa tcttattttc
    25201 aatacgtgcg tgggcaactt tgatatccat gcgcgtggaa ccgatggtgc gatgcacgcg
    25261 catatatttg ccatcaataa attccccttt cagggttgcc agattatctc gctgcgcttc
    25321 gatcttctca aatacctctt caaagcggct catcacaaac tttcgttgag gataaatttc
    25381 gcgcagtttc tccatcactt cgaagatatt ttgctgcaat ggcatctgat catgcccatt
    25441 cggcagcaaa atctctttgg ttacagacgc tttttccagc acgtcaaaat aactgtcgag
    25501 gcgtttgcgg aatccgtttt cgtccgcagg taagtactta ccgatggcat aacccaacgg
    25561 catcacctgc gccgtcactt cgctaccgtc actgctttgc cacaaaaact cggttttatc
    25621 cgtaccgtgg cgctcagaac atccacgcca gaacatggtg caggtaatgc caaatccatt
    25681 gtagatatgc ggcagttgcc cggacatgcc aaaggaatcg ggtaaataac ctattttcat
    25741 cggctcgcca aacgcgaggc agtcacgcat tccgtacatc agattacgga cgatggattc
    25801 cgcagaaaca atcgtggtat cggtctgggt ataccagggg ccgataatca acttgccggc
    25861 ttctaccagt ttcttcacgc ggtctttgtt ttccggtttc accgcgaaat aatcttcgag
    25921 gatcgccgtt tgcccgtcga gtacgtaata tttgtattcg ttgtcctgtt ccagtcggca
    25981 caggatctct tccatattat tgaccagcag aatgcgtgac tcttcggtgg tgaaatacca
    26041 ctcccgatcc cagtgcatat gcggggtgat gtgaacgcga gataccgctt tcatcgtcgt
    26101 ttcctttttg tttattgcat tacgccatca gtcagatagt tgccatgctt aaccgcgtga
    26161 cgccgccaga tcaggagaat tgcagtcgag attgcagccc ccaccaatgc cgcaccaaac
    26221 cagccaattg ccgccataac accgcccgcg ccattatcat gaagtaaaaa gagcgagaaa
    26281 atgccagcac cgggtgtcga aaggccgata ttcatcgcac cgacaatagc gcccgttacc
    26341 atagagccca gcacaaacga accaataacc cgcagcggat cttcaatcgc catcgggatc
    26401 gccccttcgg taatacctgc cagccctaac agccaggtgg atttcccggt ctcaatttca
    26461 aactctttaa acaggcgcgg tgcgagcatc gtggaagcgg ttacggtaaa tgccgaaacc
    26521 attttgacgg aggcgaaaat ggcatacggg ccgtaaacgc cgttcgccat tgcgcccagg
    26581 cagaatgcat aagcggcttt attcactggc cctccaaggt caaaggaaca cataaaaccg
    26641 agaatggcac ccagcaacag cgcgttactt cctgacagac cgttcagcca ggcggtaagc
    26701 gagttattga tccaggcgac aggttccccc accacaaaca gcatcagact gcccgctccc
    26761 aatgtaccga gcaccaggta gagataaaaa gtcaggaagc cattgaattt actgctaaga
    26821 cgcaagtgat ttttcatcca gcgtatcaag taaccggcta tcaatccgcc aacgaccgcg
    26881 ccgagaaacc cggagccgat catgttggcg gcaagtccag ccgcaaagcc tggcgctaac
    26941 gccggtttat ctgccagcga ataggcggta taggctgcca gcaccggtac catcaaaatt
    27001 ccgagcagcc cgccgcccag tttgcggtac atccacagcc aggagttttc ttcattaaac
    27061 agatcttgta gcccgaagat ttgcgacagt aataccgcga ccgccagcac cgtccccccc
    27121 gcgacaatca acggtacggc aaaagagatc ccgctcaaca gtgcctgttt cagctccgtt
    27181 ttgacgcttt tcaccggttg cgtatcttgc tgtacggtac gcgtctcatc gccacgctcg
    27241 agggtaagcg attgttggat caacgcttct gcatggcgaa tcggctcggc aacaggcaca
    27301 gaaagcgcag gaatgccgtt aaaacgctca ctctccttga tggcgacttc agccgcaaaa
    27361 atacaggcag ttgcactatt gagttgatcc gccgttaaac gcccttcaat accgttagca
    27421 ccttgctttt caacgtagac attcacgccg agtttgcgtc cggctttttc cagatactcc
    27481 gcagccatgt aggtgtgagc aatacccgcc gggcaggccg ttacgcaaac gatagttggc
    27541 gcgttggaaa aagaggcaga aggttgcgtg cctcccttgt catccagcgc cgagagcaat
    27601 tcatcaggcg tcgtcgccga ctgtatacgc gcccgaatct catcatccgc caggcgcgtg
    27661 gtcagcgctg tcagcagttg catgtgcgta gtacccgctt cattgggggg aatcgccagc
    27721 aacaccacta aatcaaccgc ttccgggcca tccacgcttt cccactgaag cggctcgctg
    27781 agtgtggcga ccgcaaacgc cgcttctttt accgcagcag ttttgccatg cggcacagcc
    27841 aacccttcac ctaaggcggt cgggccaagg ctttcacggc gatacacttc tttcagaaat
    27901 tgctcagtac tggaaatttt ccccagagca gcaagacgtt gagtcaacgc gtgaatggcc
    27961 tcttcacggc tggtaaagcg cgcattcaga cacaacgcat cgcggtgggt cagagtcgtc
    28021 aggttcatca tgatcctcac ttgatcgttt ttatagtcgc gccagtgtgc ccgataaaac
    28081 aataccattg cgtaatcaat ttcacttgat tgaaattatt tacattgtat ttgtattatt
    28141 tacaggatga atttatcggg cataatggct aaattcgtat taatgagatg ctaaataatg
    28201 ggacacaaac ccttataccg acagattgct gatcgcattc gtgagcaaat tgcgcgtggc
    28261 gagcttaaac ctggcgatgc gttacctacc gaatcggcat tacaaacaga gtttggcgtc
    28321 agccgggtta cggtgcgtca ggctttgcgc cagttagtcg agcaacagat cctcgaaagc
    28381 attcagggca gcgggactta cgtcaaagaa gagcgggtca attacgatat ttttcagtta
    28441 accagttttg atgaaaaact gtcggaccgt cacgtcgata cacacagtga agttctgata
    28501 tttgaagtga ttccggctga cgattttctt cagcaacagc tacaaatcac cgtgcaggat
    28561 cgtgtgtggc atgtgaagcg cgtgcgctat cgcaagcaaa agccaatggc gctggaagaa
    28621 acctggatgc cgcttgcgtt gttcccggat ctcacctggc aggtcatgga aaattcgaaa
    28681 tatcacttta ttgaagaagt gaagaagatg gttatcgatc gtagcgaaca ggaaatcatt
    28741 cctctgatgc caaccgaaga gatgagccgt ctgcttaata tcagccagac aaagccgatt
    28801 cttgaaaaag tatcgcgtgg atatttagtt gatggtcggg tgtttgaata tagccgcaac
    28861 gcttttaata ccgatgacta taaatttacc ctgatagcgc agcgaaaatc atcgcgataa
    28921 gcacaaaaaa ggccatcata cgatggccaa ccatgtcgaa accgacagcc cttcgccatt
    28981 cctgtagagg gaagcggcga gggctatttc ttattacaga tatttatttc agaacagttt
    29041 tcagtgcttc accgatatcc gccaggctgc gaacggtttt cacgcctgcg gcttccagag
    29101 cagcgaattt ctcatccgca gtccctttcc caccggcaat gatcgcaccc gcgtggccca
    29161 tacgtttgcc tttcggcgca gtcacaccag cgatgtaacc cacaactggc ttggtaacat
    29221 gctctttgat gtacgctgcc gcttcttctt cagcgctacc gccgatctca ccgatcatca
    29281 cgatcgcttc ggtctgcgga tctttttcga acatttcgag aatgtcgata aagttagagc
    29341 cagggatcgg gtcaccgccg ataccgacac aggtcgactg accgaaaccg taatccgtgg
    29401 tctgtttaac cgcttcatag gtcagtgtac cggaacggga aacgataccc actttacccg
    29461 gtttgtgaat gtgacccggc tggataccga ttttgcattc acccggggtg ataacgcctg
    29521 ggcagttcgg gccgatcata cgaacgcctg cttcgtccag tttcactttc acggtcagca
    29581 tatccagcgt cgggatgcct tcagtgatgg tgataatcag tttgatgcct gcatcgatag
    29641 cttccagaat ggagtctttg cagaacggtg ctggtacgta aataacagac gcggaagcgc
    29701 cagtagcagc aacagcttca cgcacggtgt tgaacaccgg caggccgagg tgggtagtgc
    29761 cgcctttacc tggagttacg ccaccaacca ttttagtgcc gtatgcaatg gcctgttctg
    29821 agtggaaagt cccctggcta ccggtaaagc cctggcagat aaccttggtg tttttatcaa
    29881 ttaaaatgga cattatttcc cctccactgc ggcaacaacc tgctgagctg catccgtcag
    29941 accttttgct gcaataatat tcaggccgct gtcagccagt ttcttcgcgc cgagttcggc
    30001 gttgttacct tccagacgta ccacgaccgg tacgttaaca cccacttccg ctaccgcgcc
    30061 gatgataccg tcagcgatca ggtcgcaacg aactataccg ccgaagatgt taaccagaac
    30121 ggctttcact ttgtcgtcag agaggatgat tttgaacgct tcggttacac gttctttggt
    30181 tgcgccgccg ccaacgtcca ggaagttagc cggttcgccg ccgtgcagtt taactatgtc
    30241 catcgtaccc atcgccagac ctgcgccgtt aaccatacaa ccgatgttac cgtccagcgc
    30301 aacgtagttc agttcccact gtgcagcctg tgcttcacgc ggatcttcct gcgactggtc
    30361 acgcatttcg cgcagatcag gctggcggaa cagggcgttg ccatcagcgc ccagtttgcc
    30421 gtcgaggcaa atcagatcgc cctgtttggt gatgaccagc gggttgattt cgatcagcgc
    30481 caggtcgcgc tccaggaaaa tggtcgccag gcccatgaag attttggtga actgctgaac
    30541 cagtttacct tccagaccca gtttgaacgc cagctcgcgt ccctgatacg gcatcgggcc
    30601 agtcagcgga tcaagcgcaa ctttatggat caggtgcgga gtttcttccg ccactttttc
    30661 gatttccacg ccgccttcgg tggaggccat aaagaccacg cgacgggaac tacggtctac
    30721 aacggcaccg agatacagct ctttagcgat atcggtcgcc gcttcaacca gaatctggtt
    30781 aaccggttgg ccattggcat ctgtttgata cgttaccaga cgcttgccca gccagttttc
    30841 tgcaaaagca cggatgtctt ctttgctgtt tacaactttc acaccgcccg ctttaccgcg
    30901 gccaccagcg tgaacctgac atttcactac ccacggaccg gcaccgattt ttgaagcggc
    30961 ttcttctgct tcgcgcggag tagtacaggc ataacccacc ggtgctggta agccatagcg
    31021 ggcaaacagt tgttttgcct gatattcatg taagttcatg tgttctgtcc atccttcagt
    31081 aatcgttatc ttttaaaccg tagaccgaca ccctatccac gtcggtctac agtgcaggtg
    31141 aagcttaaac tactacacgt ccagcagcag acgcgtcggc tcttccagca actctttgat
    31201 cgttaccagg aagcccacgg attcgcgacc atcgatcaga cggtgatcgt aggacagcgc
    31261 caggtacatc atcggcagga tctcaacctg accattcacc gccatcggac gatctttgat
    31321 agcgtgcata cccagaattg cgctctgcgg cgggttgatg atcggcgtag acatcaggga
    31381 accgaacaca ccaccgttgg tgatggtgaa gttaccaccg gtcagatctt caaccgtcag
    31441 cttgccatca cggcctttga ctgccagctc tttgattttc ttctcgatgt ctgccatgcc
    31501 gagggtatcg acatcacgca gaaccggcgt caccaggccg cgcggcgtag aaaccgccat
    31561 gctgacgtcg aaatagttgt ggtaaaccac gtcatcgccg tcgatagacg cgttcacttc
    31621 cgggtaacgt ttcagggctt caaccaccgc tttcacgtag aaggacataa agcccagacg
    31681 gatgccgtgg cgtttttcaa acgcttcacc gtactgctta cgcagatcca taatcggctt
    31741 catgttgact tcgttgaacg tggtcagcat ggcggtggag tttttcgctt ccagcagacg
    31801 ctctgccaca cgcttacgca ggcgagtcat cgggacacgt ttttcactac gtgcagccag
    31861 agccggttgc gccgccggag cagccgctgc cggagcagac tctttcgccg gggctttcgc
    31921 cagatgtttt tccacatctt cacgagtcag acgaccaccc acaccggtgc ctttaatggc
    31981 gctggcgtcg agattgtgtt cagccagcag gcgacggatc gccgggctta acgcatcgtt
    32041 gttttgctct tccagagacg cctgctggcg ttgcgccgga gtggacgctt tctcttcaga
    32101 tttggcgctg gtttctttac cggcgctgtt gccttcacgc aggcgaccaa ggatctgacg
    32161 agacgttacc gttgtacctt catcttccag aaccgcatcc agaatgccgt ctgctgatgc
    32221 cggtacttcc agtaccactt tgtcagtttc gatttctacc agcacttcat cacgtacgac
    32281 tgcgtcgccg ggttttttat gccaggttgc gacggtggca tcggctacgg attcaggcag
    32341 gtcagggacc agaatatcta cgctactcat tgtgtatcct ttatttattc gacgttcagc
    32401 gcgtcattaa ccagatcttg ttgctgtttc tggtgaacgg acatataccc taccgccgga
    32461 gaggcggagg ccgggcggcc tgcataacgc agagaagccc caaacggaat cacttcacgg
    32521 aaatgatgct ggctgcagta ccatgcgccc tggttgagcg gctcttcctg gcaccagaca
    32581 aaatccttga cgtgagcaaa ctgctgcaac acttcctgca tcgctttatg cgggaacggg
    32641 tagagttgct cgatacgcac aatggcgaca tcgtgttgat tgttcttacg acgctgttcc
    32701 agcaggtcgt aataaacctt accagaacac atcactacgc gcttcacgcc cttcggatca
    32761 agttcgtcga tttcaccgat ggctggcagg aaggtgccgt tcgccagttc ttcgaggctg
    32821 gaaaccgcca gcggatgacg cagcagggat ttcggcgaca tcacgaccag cggacgacgc
    32881 atcccgcgca gcgcctgacg acgcagcatg tggtaaacct gtgccggggt agacggtacg
    32941 caaacctgca tgttttgctc agcacaaagt tgcagataac gttccagacg cgcggaggag
    33001 tgctccggcc cctgcccttc gtaaccgtgc ggcagcaaca tcaccagacc acacatccgg
    33061 ccccatttct gctcgccaga ggagatgaac tggtcgataa ccacctgcgc accgttggcg
    33121 aagtcaccga actgcgcttc ccagatggtc agggtgcgtg gttctgcggt ggcataacca
    33181 tattcaaacg ccagcacggc ttcttctgac agtacggagt cccagacacg gaacgcgccc
    33241 tgaccgttat ggatgtgttg cagcggcgtg taagtggaac cgttagactg gttgtggatc
    33301 accgcgtggc ggtggaagaa ggtaccgcga ccggagtctt cacccgacag gcgaaccgga
    33361 atgccttcat caaccagcgt ggcgtaagcg aggttttccg caccgcccca gtcgaacagt
    33421 ttctcaccgg ctgccatcgc ctggcgatcg ccataaatct tggcaacgcg agactgcatt
    33481 tcaactgctt ctggtaccgt actgatacgt ttagccagtt cctgcaggcg cttcatctca
    33541 actttgttcg ggtactcttc gtcccattcg tggttgaggt acggcgacca ggtgaaagag
    33601 tgcatgttca tcggacgcca ctctgccact acgcaatcgc cagcatccag cgcatcgcgg
    33661 tacaggttaa ccatctcggt ggcatcttcc agcgtcgcca ctttttcctg ctccagcttg
    33721 tcagcgtaga ttttgcgcgg tgtcggatgt tttttgattt tctgatacat cagtggctgg
    33781 gttgcgctcg gctcgtcggc ttcgttgtgg ccgtgacggc ggtagcatac caggtcgatg
    33841 aagacatcac gtttaaaggt gttacggaaa tcgagcgcca gacgggtcac aaaggcaacg
    33901 gcttccggat catccgcgtt aacgtggaaa atcggtgcct gaaccatctt accgatatca
    33961 gtacagtacg gtgtagaacg ggcatccagc gggttagagg tggtgaaacc aacctggttg
    34021 ttgataacga tacgtaccgt accgccaact tcataaccac gcgctttcga catgttcaga
    34081 gtttcctgaa ccacgccctg cccggtcact gcggcgtcac cgtggatggt gattggcagc
    34141 actttgttgc tgctcggctc atcaagtctg tccagacggg cacgaacaga gccgataacg
    34201 accgggctta caatctcaag gtgagacggg ttaaacgcca gcgccaggtg caccaggccg
    34261 ccatcggtct ggaagtcaga cgagaagccc atgtggtatt tcacgtcgcc tgtgccgagg
    34321 tgttctttat gtttaccggc gaactcgtcg aacaagtctt gcggtttttt acccagcacg
    34381 ttcaccagca cgttcagacg accacggtgc gccatcccga gaaccacttc gcgggtgccg
    34441 ctgttgccag cgtggcggat catctcttta agcatcggga ttaacgcgtc accgccttcc
    34501 agcgagaagc gttttgcgcc agggaatttt gcgccgaggt aacgctcaag gccttcagcg
    34561 gcggtcagtt cgcttaagaa gcgttttttc tcttcgctat tgaaagtcgc gcgaccagac
    34621 tcgatacgct gttggatcca gcgtttttct tcggtgctgg tgatgtgcat atactcggca
    34681 ccaatcgggc cgcagtaggt ttgcttgagg gcggaaataa gctcgccgag tttcatggtt
    34741 tctttgccgc tggcaaatga accgacgttg aaggtttcct ggaagtctgc ttcggtcaga
    34801 tcgtggaaag acggatccag atcggccact ttatcttgct gccacagtcc cagcggatct
    34861 agattcgcat gctggtgacc acggaagcgg tatgcgttaa tgagctgcag gactttaacc
    34921 tgcttcacat tggtgtcagg gtcggagatc gttgaagagt aacgtgaagc gtctttcgcc
    34981 aggcggcgga aatattcacg cgtttgagag tggaattgat ccggtttgac tcccgtacca
    35041 ggtaactgct ggaacgtcga acgccagtta gcgtcaaccg agtcaggatc ggttaagaag
    35101 tcttcataga gctgttctat ccagctctgg tttgcgccag agaggtaaga agagtccaac
    35161 caggctttca aagcgctgtt ctgcatcgtg atcccttaag catctttttt atgcttactt
    35221 cgccgtggat actaccacgc acagtgatgt gcgtgccggg gttcacttgc gagctcttgt
    35281 ctacgtagtg gctcgcgaag gaacctttaa aaactgtctt agtgtcaggg gcagttttta
    35341 aagatttcct gcatttcttt gttacgcctg atgcgctgcg cttatcaggc ctgcgggtaa
    35401 tgacttgtag gccggataag gcgttcacgc cgcatccggc actggttgcc tgatgcaacg
    35461 cttgcgcgtc ttatcaggcc tacagtttac gcattacgtt gcaacaacat cgacttgata
    35521 tggccgatgg cgcgcgtcgg gttcagcccc ttcggacata cactgacgca gttcatgatg
    35581 ctgtgacagc ggaatacgct gaatgcatcg ctcaaaccgt cgaggcggct gtcagtctcg
    35641 gtatcacggc tatcgatcag gaaacgatat gccgctaaca agcctgccgg gccgataaac
    35701 ttatcgggat tccaccagaa agacggacaa gaggttgaac aacatgcgca gagaatacat
    35761 tcatacagcc cgtcgagttt ttcgcgctgc tctggcatct gtaaatgctc gcgagctggc
    35821 ggattttgtc cattattcaa caggtaaggc ttaattttct catattgcgc atagaattgt
    35881 cccatgtcta ccaccaaatc gcggatcacc ggtaaacctg gcagcgggcg aatcacaatc
    35941 ttcttgcccg gctggttgag tgccgaaatc ggggtaatac aggccagacc attcttgccg
    36001 ttcatgttca gaccgtcgga accgcacaca ccttcacggc aggagcggcg gaacgacagg
    36061 ctgggatctt tctctttcag ctggataagc gcatccagca gcatcatgtc gcgaccttct
    36121 tccgcttcca gggtgtaatc ctgcatacgc ggagcatcat caacatccgg gttatagcga
    36181 taaattgaaa actcgagtct cattttcctg tctccgcatt agtaagtacg aatcttcggc
    36241 gggaatgccg ggcgcagttt cggttccatg ttgacgcttc ggcgcgtcat ggattccgac
    36301 tcaggcagat acagggagtg gcacagccag ttttcatcat cacgatccgg gaagtcgaag
    36361 cggctatgtg cgccacggct ttcggtacgg aagttggcag aaacagccgt tgcatacgcc
    36421 gtttccatca ggttatccag ttccaggcac tcaacgcgct gggtgttgaa ctcgctggaa
    36481 gtgtcatcca gacgggcatt tttcagacgc tcgcggatca ctttcaactg ctcaagccct
    36541 ttcgccatcg catcaccttc acggaagacc gagaagttat gctgcataca ttcttgcagc
    36601 gctttacgga tcgccaccgg atcttcaccg ttacgattat tgttccaccg gttcaggcga
    36661 tccagagacg cttcaacatc agactcgctg gcatcgcgca gtgcgccctg ctcggcgata
    36721 gactcttgca gatgcagacc tgccgcgcga ccaaagacca ccaggtccag cagcgagttg
    36781 ccgcccagac ggttagcgcc gtgtaccgat acacaagcga tttcaccaac ggcaaacagt
    36841 cccggaacaa ccacatcttc gcctttctca ttcacagtca gcgcctgacc ggtaactttg
    36901 gtcggaatac cgcccatcat gtagtgacag gttgggataa ccggaatcgg ctctttcacc
    36961 ggatcgacgt gagcgaaggt acgggaaagc tccaggatac ccggcagacg ggattcgaga
    37021 acttctttac ccaggtgatc gagtttcagt ttcgcgtgtg gcccccacgg accatcacag
    37081 ccgcgacctt cacggatttc aatcatgatg gaacgcgcca ccacatcacg gcccgctagg
    37141 tctttggcgt tcggcgcata acgctccata aagcgttcgc catgtttgtt cagcagataa
    37201 ccgccttcac cacggcaacc ttcggtgacc agtacgcccg caccggcaat gccggtcggg
    37261 tggaattgcc acatttccat atcctgcacc ggtacgcctg cgcggatagc catgccgaca
    37321 ccgtcgccag tgttaatgtg ggcgttagtg gtggactgat aaatacgccc tgccccgcca
    37381 gtcgccagca cggtagcgcg ggctttgaaa taaaccactt caccggtttc gatgcacagt
    37441 gcggtacaac ccaccaccgc gccatcctgg tttttcacca gatccagcgc ataccactcg
    37501 gagaaaatgg tggtgtggtt tttcaggttc tgctgataaa gcgtgtgcaa cagtgcgtga
    37561 ccggtacggt cagcagccgc cgcagtgcgt gccgcctgct cgccgccgaa gtttttcgac
    37621 tgaccgccaa acggacgttg atagatacga ccatcatcga gacgcgagaa cggcaggccc
    37681 atgtgttcga gttccagaat cgcttccggc ccggttttac acatatattc aatcgcgtcc
    37741 tggtcaccga tatagtccga cccttttacg gtgtcgtaca tatgccattc ccagttatct
    37801 tcatgggtat tacccagcgc aacggtaatg ccgccttgcg cagaaacggt atgggaacgg
    37861 gtcgggaaga ctttagagag cagcgcacag gtctggccgc tctgggaaat ttgcagcgcc
    37921 gcgcgcatac ctgcgccacc ggcaccaatc acaactgcat caaattctct gactggcaat
    37981 ttcatcacac cccccacacc acaacgaatc cataaatcac gtaaaccacc agtgcaacga
    38041 caatcaccag ttgcagcatc aggcgcaagg ccagcggttt aacgtagtcg gtcaacacct
    38101 gccacatgcc gatccaggca tgaatcaaga tagaaaacag cgccagcagg gtgaacactt
    38161 tggtgaacgc agaggcgaag aaaccgatcc agacttcata tgtcagttcg ccactggtgg
    38221 cgaaaaaacc gaccatataa atgatgtaga gcgtcaggac gatagcggta gcgcgaacga
    38281 ggatgaaatc atgtacgcca ttgcgtccta atgcggaggc gttgcttacc atacgaggac
    38341 tcctgcgaga agtgaaagca cgacagtaat aacaaaggag attttggcgg agcgtttacc
    38401 cgcttcgaat gtttcttcca gatagccaaa atccatcatc atgtggcgaa tacctacgac
    38461 gacgtgatac gccagagcgg taaggatgcc ccacatgata aatttgacga agaagctgcc
    38521 cataatcgcg gaagcttgct cgaaaccttc aggggaagag aggctggtac ccagaagcca
    38581 cagcaggatg cccactgcaa caaaggtgat cacaccggaa acgcgatgga gaatggacgc
    38641 tatcgccgtg atggggaacc ggatggtctg taggtccaga ttaacaggtc tttgtttttt
    38701 cacatttctt atcatgaata acgcccacat gctgttctta ttattccctg gggactacgg
    38761 gcacagaggt taactttctg ttacctggag acgtcgggat ttccttcctc cggtctgctt
    38821 gcgggtcaga cagcgtcctt tccataactg cgcgtcatgc aaaacactgc ttccagatgc
    38881 gaaaacgaca cgttacaacg ctgggtggct cgggattgca gggtgttccg gagacctggc
    38941 ggcagtatag gctgttcaca aaatcattac aattaaccta catatagttt gtcgggtttt
    39001 atcctgaaca gtgatccagg tcacgataac aacatttatt taatttttaa tcatctaatt
    39061 tgacaatcat tcaacaaagt tgttacaaac attaccagga aaagcatata atgcgtaaaa
    39121 gttatgaagt cggtatttca cctaagatta acttatgtaa cagtgtggaa gtattgacca
    39181 attcattcgg gacagttatt agtggtagac aagtttaata attcggattg ctaagtactt
    39241 gattcgccat ttattcgtca tcaatggatt ctttacctgc aagcgcccag agctctgtac
    39301 ccaggttttc ccctctttca cagagcggcg agccaaataa caaacgggta aagccaggtt
    39361 gatgtgcgaa ggcaaattta agttccggca gtcttacgca ataaggcgct aaggagacct
    39421 taaatggctg atacaaaagc aaaactcacc ctcaacgggg acacagctgt tgaactggat
    39481 gtgctgaaag gcacgctggg tcaagatgtt attgatatcc gtactctcgg ttcaaaaggt
    39541 gtgttcacct ttgacccagg cttcacttca accgcatcct gcgaatctaa aattactttt
    39601 attgatggtg atgaaggtat tttgctgcac cgcggtttcc cgatcgatca gctggcgacc
    39661 gattctaact acctagaagt ttgttacatc ctgctgaatg gtgaaaaacc gactcaggaa
    39721 cagtatgacg aatttaaaac tacggtgacc cgtcatacca tgatccacga gcagattacc
    39781 cgtctgttcc acgctttccg tcgcgactca catccaatgg cagtcatgtg tggtattacc
    39841 ggcgcgctgg cggcgttcta tcacgactcg ctggatgtta acaatcctcg tcatcgtgaa
    39901 attgccgcgt tccgcctgct gtcgaaaatg ccgaccatgg ccgcgatgtg ttacaagtat
    39961 tccattggtc agccatttgt ttatccgcgc aacgatctct cctatgccgg taacttcctg
    40021 aatatgatgt tctccacgcc gtgcgaaccg tatgaagtta atccgattct ggaacgtgct
    40081 atggaccgta ttctgatcct gcacgctgac catgaacaga acgcctctac ctccaccgtg
    40141 cgtaccgctg gctcttcggg tgcgaacccg tttgcctgta tcgcagcagg tattgcttca
    40201 ctgtggggac ctgcgcacgg tggtgctaac gaagcggcgc tgaaaatgct ggaagaaatt
    40261 agctccgtta aacacattcc ggaatttgtt cgtcgtgcga aagataaaaa tgattctttc
    40321 cgcctgatgg gcttcggtca ccgcgtgtac aaaaattacg acccgcgcgc caccgtaatg
    40381 cgtgaaacct gccatgaagt tctgaaagag ctgggcacca aagatgacct gctggaagtg
    40441 gctatggagc tggaaaacat cgcgctgaac gacccgtact ttatcgagaa gaaactgtac
    40501 ccgaacgtcg atttctactc tggtatcatc ctgaaagcga tgggtattcc gtcttccatg
    40561 ttcaccgtca ttttcgcaat ggcacgtacc gttggctgga tcgcccactg gagcgaaatg
    40621 cacagttacg gtatgaagat tgcccgtccg cgtcagctgt atacaggata tgaaaaacgc
    40681 gactttaaaa gcgatatcaa gcgttaatgg ttgattgcta agttgtaaat attttaaccc
    40741 gccgttcaaa tggcgggtta atttttatat gcctaaacac aaaaaattgt aaaaataaaa
    40801 tccactatcg gacattcata tattctaaaa aaaaatatat cggtataaat tataaacaaa
    40861 tcgatgattt ttaatcatct atcttcctct cagttacatg aataaaccac caaatatagc
    40921 gcacccctat aatttccaat actttattca tcaaacattt aattcatacc atcaattaat
    40981 tacttttaca tttaataaaa ttaataatat ttttaagaaa aactaaaaac attcgacaat
    41041 caaataagat attcttgcac cgatatactt ttttcttttc tgttaaagga ctcagttatg
    41101 tttaaaggac aaaaaacatt ggccgcactg gccgtatctc tgctgttcac agcacctgtt
    41161 tatgcggctg atgaaggatc cggtgaaatt cactttaaag gtgaagttat tgaagcaccg
    41221 tgtgaaatac atcaggatga tattgataaa gaggttgaac tcggtcaggt gaccaccagc
    41281 cacattaatc agtcacatca cagcgatgcc gttgctgtcg acctgctctt agtcaactgt
    41341 gatctggaaa actccagcaa cggttccggt ggcaagattt ctaaagttgc agtcaccttt
    41401 gatagctcag cgaaaaccac cggcgcagat ccgattctca acaacaccag cacgggtgaa
    41461 gccaccggcg tcggcgtacg tttaatgaat aaagatcaaa gcaatatcgt tttaggcacc
    41521 gctactccag atatcgacct gctccgacct ccagcgaaca aacgctgaat ttctttgcct
    41581 ggatggaaca aattgatcag gctacacctg taacgccagg cgcagttacg gcgaatgcaa
    41641 cctacgtgct ggattataaa taagtattac tttggcggcg ctatcgccgc ctttcataaa
    41701 atatttatca ggtatggaca ccgtgaatat ttatcgactc tcttttgtat cctgcctggt
    41761 cgtggcgatg ccttgcgcat tggcggtcga attcaatctg aatgttctcg ataaatcaat
    41821 gcgcgaccgc attgatattt cattattaaa ggaaaaagga gtcattgctc ccggtgagta
    41881 ttttgttagc gttgcggtaa ataataacca aatcagtaac gagcaaaaga ttaactggca
    41941 caaaaatgac gataaaacca ttccgtgcat caatgattta ctggtcgata aatttggctt
    42001 aaaacctgaa gtccgtcagt cattaccatt gataaatcag tgcgtcgatt ttagctcccg
    42061 acctgaaatg ctcttcaatt tcgatcaagc caatcagcaa ctgaatatta gtattccgca
    42121 agcctggctg gcgtggtact cagaaaactg gaccccaccc tccacatgga aagaaggtgt
    42181 cgccggtgtc ctgatggatt acaacttgtt tgccagcagc taccgcccac aggatggcag
    42241 cagcagcact aatcttaacg cctatggaac agccggaatc aacacggggg catggcgctt
    42301 acgcagtgat tatcagttga atcatactga tagcgatgat aaccatgaac agtcaggcga
    42361 aatatcacgt acctatcttt ttcgcccgtt accgcaatta ggttcaaaat taaccctcgg
    42421 cgaaacggat tttagttcca atattttcga cggtttttct tataccggcg cggcactggc
    42481 aagtgacgat cgaatgttgc catgggaact acgcggctac gccccacaaa ttagcggtat
    42541 tgcacagacc aatgccacgg tgacgatcag tcaatcaggc cgcgtcattt accagaaaaa
    42601 agtcccacca ggcccattta tcattgacga ccttaatcag tctgttcagg gcacactgga
    42661 tgtcaaagtg acggaagaag atggtcgggt gaacaatttc caggtttcag cagcatcgac
    42721 gcccttcctg actcgtcagg gacaggttcg ctataaactg gccgcgggtc agccacggcc
    42781 ctccatgtca catcaaactg aaaatgaaac cttttttagc aatgaagttt cctgggggat
    42841 gctgtcaaac acctcgctgt acagcggcct gctgctttct ggtgatgact accattctgc
    42901 cgcaatgggt attgggcaaa atatgctgtg gcttggtgcg ctgtcgtttg atgtcacgtg
    42961 ggccagtagc cattttgata ctcagcagga cgagcggggc ttaagctacc gttttaatta
    43021 cagcaaacaa gtggatgcca ctaacagcac gatttccctc gccgcttatc gtttctccga
    43081 tcgtcatttt cacagctacg ccaactatct ggatcacaaa tacaacgaca gcgatgcgca
    43141 ggacgaaaaa cagacgatca gcttatctgt gggccaaccg attaccccac taaacctcaa
    43201 tctttacgcc aacctgctac atcaaacctg gtggaatgca gacgcctcca cgaccgccaa
    43261 catcacggca ggttttaatg ttgatattgg tgactggaga gatatctcta tttctacgtc
    43321 attcaataca acccattacg aagataaaga tcgcgacaac cagatttacc tgtcgatttc
    43381 gctccccttc ggtaacggtg gtcgggttgg ctatgacatg caaaacagta gccacagcac
    43441 cacacaccgc atgtcgtgga acgatacgct ggatgaacgt aatagctggg gcatgtctgc
    43501 cggactgcaa tccgaccgtc ctgacaatgg agcccaggtg agcggtaact atcagcacct
    43561 gagttcagcg ggtgagtggg atatttctgg tacctatgcc gccaatgatt acagttccgt
    43621 cagcagcagc tggagcggtt ctttcaccgc aacccaatat ggtgcagcgt ttcatcgccg
    43681 cagctccacc aatgaaccac gcctgatggt cagcaccgat ggcgtggcag atattccggt
    43741 tcagggcaat ctcgactaca ccaaccattt tggcattgcg gtggtgccgt tgatttccag
    43801 ttaccagcct tccaccgtgg cggtgaacat gaatgactta cccgacggcg taacagttgc
    43861 agaaaacgtt atcaaagaaa cgtggattga aggcgcgata ggttacaaat cactggcttc
    43921 ccgttccggt aaagacgtta acgtcatcat tcgcaacgcc agcggtcagt ttcctcccct
    43981 cggagcggat atccgccagg atgacagcgg cattagcgtg gggatggttg gcgaggaagg
    44041 acatgcctgg ttaagcggag tcgctgaaaa tcaaaagttt accgtggtct ggggtgatag
    44101 ccagcattgc tcgctccatc ttcctgaaca tatggaagac accgcaaatc gcctgatttt
    44161 accttgtcat taattaagga aatgaaaatg acatttatga aaggactacc ccttttgttg
    44221 ttggttgcca gtctgtgcag ccacgctgca ctacaacccg atcgcactcg tattgtgttt
    44281 aacgctaatg ataaagctac cagtctgcgg gtggataatc gtagcgataa actcccctac
    44341 ctcgcttatt cctggctcga aaatgaaaaa ggggagaaaa gcgatgatct tctggtcgcc
    44401 ctgccgccga tacaacgtct ggaaccgaaa gcaacaacgc aggtacggat agtaaagcaa
    44461 gcctcgacca caaaattacc gggcgatcgc gaaacgctat ttttctacaa tatgcgtgaa
    44521 attcctcccg ccccggaaaa aaacagcgac catgccgttc tgcaagtcgc cattcagagt
    44581 cgtatcaagg tgttctggcg accggctgca ttacgcaaga aagcaggaga aaaggtcgaa
    44641 ctgcagttac aggtcagcca gcagggcaac caactgacac tgaaaaatcc taccgcgtat
    44701 tatctgacca ttgcctacct gggacgaaat gaaaaaggcg ttctccccgg cttcaaaacc
    44761 gtgatggttg cgccttttag tacggtcaac acaaacaccg gaaattatag cggcagccag
    44821 ttttatctcg gttatatgga tgattacggc gcgttacgca tgacgacgct caactgcagc
    44881 ggacaatgcc atttacaagc cgtggaggcg aagaaatgag tgctggcaag ggagtgttgc
    44941 tcgtcatctg tctgttattt ctgccgttga agtctgcgct tgccctgaac tgctactttg
    45001 gttcatccgg tggctcggta gaaaaatcag aagcgattca accttttgct gtaccaggca
    45061 atgccaaact cggcgataag atctgggaat ctgacgatat taaaattccc gtctattgtg
    45121 acaacaatac caacggcaac tttgaaagcg aacatgttta cgcatgggta aacccctacc
    45181 cgggggtgca ggatcgttat tatcaactcg gagtaactta caatggcgtt gattatgacg
    45241 ccaaccaggg aaaaagccgc atcgacacta accagtgtat cgacagtaag aacattgaca
    45301 tttatacccc tgagcagatc atcgcgatgg gatggcagaa caaaatttgc tcgggtgatc
    45361 ccgctaatat tcacatgtcg cgtacctttc tcgctcgcat gcggttatac gtcaaaatac
    45421 gagaaatgcc gccgcatgat tatcaaagta cgcttagcga ctatatcgtc gtgcaatttg
    45481 acggtgccgg tagcgttaac gaagacccta ctgcccaaaa cctgaaatat catattactg
    45541 gtctggaaaa cattcacgtg ctggattgca gcgtcaattt ttccatttca ccggaaacac
    45601 aggtgattga ttttggtaaa tttaatttgc tggatattcg tcgccacaca atgtcgaaaa
    45661 cattcagtat taagacgaca aaatcacaaa atgaccaatg caccgacgga tttaaagtca
    45721 gttcctcctt ttataccgaa gaaacgttgg ttgaagaaga taaagcattg ttgattggta
    45781 atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc ttataccttc aataaatatg
    45841 ctgaatacgc cgatttcacc agcgacatgt tggtctacga aaaaacctat acagctgaac
    45901 tttcgtccat tgcaggtacc cccatcgagg ctgggccctt cgatacagtg gtgcttttta
    45961 aaattaacta taactgattt gccatgatgc cctttgcagc cggaaagggc atctctttca
    46021 gaaaattgct gattttcccc ttttccttga ttgctgttat tttttcctag cccggctttc
    46081 tttatgctgt atcgatacac caaagagaaa ataatgaggg agcgaaggat gccagttttg
    46141 cagtggggaa tgttatgtgt gctatcactt ctcctttcta ttggtttcct cgcggtacat
    46201 ctcccggcgg cgctattact tgggccgatg atcgccggga tcatcttcag tatgcgcgga
    46261 attactctgc aacttccccg ctccgctttt ctcgccgcgc aggccattct tggctgcatg
    46321 attgcgcaaa acctcactgg ttcgattctc accacgctgg ccgccaactg gccgatcgtg
    46381 cttgctattt tactggtgac gctgctttcc agcgccatcg taggttggtt attggtgcgc
    46441 tatagctcac tgcctggaaa taccggtgcc tggggttctt ctccaggcgg cgcagcggca
    46501 atggtcgcta tggcgcaaga ttacggcgca gatattcgcc tggtggcgtt tatgcaatat
    46561 ctgcgagtgc tgttcgtcgc gggggccgct gttctggtca cccgcatgat gctgggtgat
    46621 aacgctgaag cggttaacca gcaaatcgtc tggttcccgc cagtgagcat taatctcctg
    46681 cttacgattt tgctcgcagt cgttgccggt acggcgggat gcatgctgcg tcttccttcc
    46741 ggcacgatgc tcatcccgat gctggcgggc gcagtgctcc agtctggtca gctcatcacc
    46801 atcgaactac cggaatggct gctggcgatg gcgtatatgg caattggctg gcggattggt
    46861 cttggtttcg ataaacaaat cttactgcgg gcactacgcc cgctaccgca aatcctgctg
    46921 tcgatttttg ctctgctggc tatttgtgca ggtatggcgt ggggactgac ccggtttatg
    46981 catattgatt ttatgactgc ctacctcgcc accagtcccg gcgggcttga taccgtggcg
    47041 gtcatcgccg cagggagcaa tgccgatatg gcgcttatta tggcgatgca aaccctgcgc
    47101 ctgttcagta ttttgctgac agggccagcc attgcgcggt ttatttcaac ctatgcgccg
    47161 aagcggtcgg cctagtgctg gcagcctgga caccagtaaa acgggcgcga ggacaacgtg
    47221 gttttctcaa tgatgccgcc acagcgttcg cacagttcgc catcacgatg aaaaacctta
    47281 aagcgaaaca gcgccccatg atgcttattc tcatccacct gcccccgcgt agcgtaagaa
    47341 aaccgaggaa tctccagtaa cgcgtgtgcg agtgcatcca gttgcgccgc attgagatct
    47401 ttcgctttat gatttccagt caacccaacc tgccagagga tctccacccg caaataattg
    47461 ccaagcccag ccagaaacgc ctgatcgagc agtaatcctg caaactgacg gttacgaaag
    47521 cgcggcgaca ataaccgttc ctttaccacc tccggcgtca gattcggatc cagcacatcg
    47581 ggaccaacgc gttgtaaaaa cggatgcgtg gtcagttgtt ccggcctcaa catctcaata
    47641 tccgaggcgc tataaagcag aatggttttg tcagccgttt gcagttttac ccgcaatact
    47701 cgcgtggtct gcggctcttc gccggtatca accacgcgcc agacgccgta aagctgatta
    47761 tggctgtaga gcgttaaacc gttgggaaaa tgagttaaca acgccttacc acgcgtttcc
    47821 acatgggtaa cgtgttgacc gataagttgt gattgataag tttttaactg cgggaaggca
    47881 aaccagacat cagttagtgg tttgcctttg atcgccgcct ccaggttatc cgctgcacgg
    47941 cggatctccg ggccttcagg catggtgata tccttttttc attctttatt aagcggggtt
    48001 atgctgcgac aacaatcccc tttttggcaa atgtagctcg taatcggcgg gcgaacgcca
    48061 gagcgtgctc gccgtcgcca tgcaggcaga ccgtttgcgc cgcgaccgtt gcccattcgc
    48121 cggtgatact tttgactctg ccgtgttgca ccatctccag cgtttgcgcc aacgcctgtt
    48181 cttcgttttc aatcaacgcg cctggctgac ttcgcggcac cagcgagccg tcagcctgat
    48241 aaccgcgatc ggcaaacact tcctcgcgcg ttgtcaggcc atattgcttg cctgcacgaa
    48301 tcagctcgct tcctgccagc ccaacgagaa ccaatgccgg atcgcaagcg tataccgctc
    48361 tggcgatggc gtctgccagt tgtgtttctt tcgccgcctg gttgtacaac atgccgtgcg
    48421 gtttgacatg acgcattacg ccgccttgcg cacgggcaat cgttgccagc gcgccaattt
    48481 gatacagcgt ctgggcgtaa acggtttccg gcggcagctg catggcgctg cgaccaaaat
    48541 tttccctgtc gggaaaactc gggtgagcgc caatcgcgac accatttttt attgcttcac
    48601 gcacgcaagc ctgcatggtt tgcgcatcgc ctgcatgaaa tccacaagca atattggcag
    48661 aggaaaccag cgttaatagc tctgcgtcgc tggcgcagcc ttcgcccaga tcggcgttca
    48721 ggtcaatttt cattgtgcag ccgccacgct aattgttcaa aataacgttg ctgatcttgc
    48781 cgcgctttca gtgcctcttc cagtgaacac tggacaaaat gaatcggctg accgagcgga
    48841 atttgcgcca gatggtacat atcagcctca atgatacagg caatacgtgg ataaccaccg
    48901 gtggtctgtg cgtcgttcat caacacaatc ggctgcccgt tatgcggcac ctgcaccacg
    48961 cccggtaaca aaccgtgaga taacagttcg cgatcggtga tgcgttttaa aatttgcccc
    49021 tgtaaacgat agcccattcg gttactttgc gagctaagct gccagggcga acgccagaat
    49081 gcatcctgcg aggcgcgatc gaactcatga tattccggcc ccggcaaggc gcgaatgcgg
    49141 ttgccccaca gcagctgttt aacgccctgc gcttccataa aatcacgctt cgatttgcca
    49201 atcgggagtc ggtcaccatc cttcagtaaa cggccttcca gcccgccaat ccccactttg
    49261 agatcggtgc tgcatgaccc cattaccggc ggaacatcaa taccacccgc gaccgccaga
    49321 taactgcgca tcccgtgctg cgggcgttta agcgttaaac gctggcctgc tttcatcggc
    49381 aatcgccagc cggtccagac ggcgttatca tccaaccgcg cttcgcaacc tgcacccgtc
    49441 agggcaaacc acccatcagt ttcgaactca actgttaact gaccgagcgt gatctccagc
    49501 gcgggggcat tggcgtcatt acccaccagc aggttagcaa tgcgcagcgc aggcatatcc
    49561 agtgcgccac agtggctgat acccgactgg cgaaaaccgt gacgaccgcc atcctgcaca
    49621 gtggtgtaca tgcccgcacg aataatcttc agcatattcc ctccttctgc ggtacaaatc
    49681 gcacgctgtc tcccggacgt aataagatgg gttcgtcacg cgccggatca aacaggctga
    49741 gtgaggtatg accaatcaac tgccagccac ccggcgttgc cagcggataa acaccagtct
    49801 gcggcccgcc gatcccgaca gaacctgccg gaacgagcaa gcgcggttca gcgcgccgtg
    49861 gcgtgtgtag ttgttccggc aaactcccga gatacgggaa gcccggttga aaacctaaaa
    49921 accagaccac gtattccacg gaggagtgca attcaacaac ctgtttttcg ctcaacccgc
    49981 aatacgccgc gaccaccgcc aaatccggtc cgcctgcacc accgtaaacc accggaattt
    50041 caataaagcg agactcaggt tccagggcct cgctctcctc ccaccagcgt tgcaaacgct
    50101 ctatggcatc cagcgccagc gactcaggat tacgcaaaat caccgtgata ttgttcatgc
    50161 cgggaatggc ttcaaccaca ttcggcatat ccaccagacg ctgcgccagt cgccagatcc
    50221 gcttctggct agccagcgtc accggcggtt ccagttccag cactaccgcc gtttcaccta
    50281 tcagataaca acgcgctcgt tgcactttcg atccctctga ttattcgtta tgcaggatta
    50341 ggaatatcaa taaaggtcac atcaagatcg gtattttcat tcagccactc gctcaatgcg
    50401 cgaataccac cgcgttcagt ggcatggtga cctgcagcat aaaaatgcaa tccctgctcg
    50461 cgggctgaat gaatggtctg ttcagaaact tcgccagtaa taaaagcatc cacgccaaaa
    50521 cgcgcggcgc tatcgataaa actttgcccg ccgcccgtgc accaggcgac gcgctggacc
    50581 acctcaggtc cggtatcgcc acaccataac ggcttacgtc ccagacgcgc ttcaatccag
    50641 gaggctagtt ccattcccgg cacaggcatg gtcagctctc cccacggcac caacggctca
    50701 atttcgccca tgaccgtgat ccccagtaac gccgccagct gtgcgttatt gcccagctca
    50761 ggatgtgcgt caagcggcaa atgccagcca tacaggttga tatcattctc cagcaacgtt
    50821 tttaaacggt tacgcttcat tccgcgaatg accggagact ctcctttcca gaagtagcca
    50881 tgatgcacaa tgaccgcatc cgcgcccaga cgcactgcct catcgagcaa agcctggctg
    50941 gcggtgacac cggtaacaat tttttgcacc gtctctttgc cttccacctg caaaccattc
    51001 ggcgcgtaat cactaatcgc cgcgctgttc agtttttcgt taatcagttg ttccagttcg
    51061 gtgtttttca tcttcgcgcc ttccgtattc tgatagctac accgttaaca tagcgttacc
    51121 tcgcctgttc gtcgatagcc aatcttttga catattaaga aacaaacccg acgccaaaag
    51181 acgcaaattg tttatatcat cacgcttcag ctaatgtata tttatgcaat cagacagcat
    51241 attgcataaa taatcagatc tgtatcaaag ttttaacttt aaggaaacac aagcacgccg
    51301 cgcaactatt acgtgacgtt gccgtcaatt gtggaattat tatgaataaa cacgcatcac
    51361 aaccgcgcgc tatttactat gtcgttgcgc tgcaaatctg ggaatatttt agcttttacg
    51421 gcatgcgtgc cctgctgatt ctctatctca ccaatcaact aaaatacaac gatactcacg
    51481 cctacgagtt atttagcgcc tactgttcgc tggtgtatgt cacgccaatc ctcggtggct
    51541 ttttggcgga taaagttctc ggcaatcgca tggcggtgat gctgggggcg ttgttgatgg
    51601 cgatcggtca tgtggtgctg ggtgccagtg agatccatcc gtcattcctc tatctgtccc
    51661 tggcgattat cgtctgcggc tatggcctgt ttaaatctaa cgtaagctgt ctgctcggcg
    51721 agctgtatga gccaaccgat ccgcgtcgtg atggcggttt ctcgctgatg tatgcggcgg
    51781 gtaacgtggg gtctattatc gcacctatcg cctgtggtta cgcccaggaa gagtacagct
    51841 gggcgatggg ctttggcctg gcggcggttg gcatgatcgc gggtctggtc attttcttat
    51901 gtggcaatcg tcatttcact catacccgcg gcgttaacaa aaaagtactg cgtgcgacaa
    51961 actttctcct gccgaactgg ggatggctgc tggttctgct ggtggcaacg cctgcactga
    52021 ttaccgtgct gttctggaaa gagtggtcgg tatacgcctt aattgtcgcg actattattg
    52081 gcctgggtgt actggcaaaa atttatcgca aagcagaaaa ccagaaacag cggaaggagc
    52141 tggggctgat tgtgacgctc accttattca gtatgttgtt ctgggccttc gcacaacagg
    52201 gcggtagctc gattagcctt tatatcgacc gcttcgttaa ccgcgatatg tttggttata
    52261 ccgttccgac cgcgatgttc cagtcgatta atgccttcgc agttatgctg tgcggtgtgt
    52321 tcctggcgtg ggtggttaaa gaaagcgtcg caggtaatcg taccgtgcgc atctggggaa
    52381 aatttgctct tggccttggc ctgatgagcg ccggattctg cattctgacc ttaagcgccc
    52441 gctggtccgc aatgtatggt cactcttctc tgccactgat ggtgttaggc ctcgcggtga
    52501 tgggctttgc ggaactgttt atcgacccgg ttgccatgtc gcaaattacg cgcattgaaa
    52561 ttccgggcgt gaccggcgta ttaaccggca tctacatgct gctttctggc gcgatcgcga
    52621 actatctggc gggcgtgatt gccgatcaga catcgcaggc ttcgtttgat gcttccgggg
    52681 cgatcaacta ctccatcaat gcatatattg aagtatttga tcaaattacc tggggcgcac
    52741 tggcgtgtgt aggactggta ctgatgattt ggctgtatca ggcgctgaaa ttcagaaacc
    52801 gcgcgctggc gctggagtca taataggcca gcaccgatgc ctgatgcgac gcttgacgcg
    52861 tcttatcagg cctacgtatt tcctgcaatt tattgaattt gcacaaattt gtaggctgga
    52921 ttaggcgttc acgccgcatc cggcatctgg gctcgattgc ctgacgcgtc tgttatttcc
    52981 ccttccgcgc cgcctcatac gctgccaacg tttgtactct cgcctctttg tgctcgacta
    53041 tcggttgcgg ataatccagc ttcacacctg ctttctgcgc ccacttccac ggctcatgca
    53101 ccgctttccc tggtacatcg cgcagctccg gtaaccatcg acgaataaac tcgccctcac
    53161 ggtcaaattt ctcgccctga gttataggat tgaaaatacg aaaatacggc gcagcatcag
    53221 tacccgtaga agccgcccac tgccagccac cgttattagc tgccaaatca ccatcaatca
    53281 gctgcgacat gaaatatcgc tcgccttcgc gccagtcgat caacaaatct ttcaccagaa
    53341 aactggctgt aatcatccgt agccggttat gcatccagcc agtgctgtta agctgacgca
    53401 tagcggcatc aataatcggg tatcccgttt tgcctttctg ccaggcctgt aaatgtgcgg
    53461 gattgctttg ccactgcaca cgatccgtcc aggcaataaa tggacgatgt ttacacaacg
    53521 aggggtaata cgtcatcaga tgacggtaga actcgcgcca gatcagctca ctaagccaga
    53581 cactaccggc cccaccgtcc agcgcctgcg gctgttccgc caacaagcga tgcaaacact
    53641 ggcgaggcga taacccgccc gttgccaagc tggcggacaa acggctggtg ccttccactg
    53701 ccggaaaatc tcgttgttgc tcatactctc cagcaccgtt ctcgcaaaac tggcgtaatt
    53761 gcgcaatcgc cgctttttct tccaccggga aatgcgcagt atcgaaagac tgacgaggat
    53821 aattcagcgt aatggatggc gcgggcttta tcgatccgct actacgaact tttggtgcag
    53881 cgacgcactc cggcatccct tcccgcagcc gtttcagcca ggcattctta aaaggcgtaa
    53941 agactttgta catctcgtga ttgccggtca tcaccgcccc cggcggcagg atcacgctgt
    54001 catcaaatcc ttcacacacc acgttacgca gcgctctttc aacgtccaca tcccgcgctc
    54061 gctcattcac ctcatactga tagttataaa aaagatgagt aacgctgttt tccgcgcaca
    54121 cctgtttaac tatttcgaca ctggcgacaa agtcatccac ttcacggaac aataaaggaa
    54181 tacctttttc cgccagcgcg atttgtagcc cattcagttg agcattgata agttcagcct
    54241 gacgcggcga catgttatgc gtctcccact ggcgtggtgt agcgatatac agcgccagca
    54301 cgcgtgcaga cgaattgcgg caggcggcag ccagtgcgag attatcgtgc agacgtaaat
    54361 cctggcgaaa ccaggccaga tgggtagtca taaaactcct gaatggcgca agttaatcac
    54421 acgcggccaa agttcgaaat aacgctgccc tgaaagccag gcgtcaggat aaagcgccat
    54481 atagtgtttg atacgcatca gcggcgcaag aagtggttgc tcgccacggc gataactgtc
    54541 gataagcgca gccagctgct ggcgttctgt ggaatcaata tgcgggcgaa aataaccctg
    54601 aacgtgcatc aaaacgttgg tgtgatcgcg cgggttggcg ggatgagaaa gcaaaacaat
    54661 tacgcgttgg cggtactggt tgtaaaaatc atccagattt tgccactgat gaatatcagc
    54721 cacaaacggg ccaagtttgc ggtactcggg ctgagaatgg gccagaaaga cgagtttata
    54781 gcggctgtga taatcgagaa gcgccccgcg cgttaagcca tgttctttca gtcgatgtaa
    54841 ctcatgaagg gcgaagatac ggattagggt gctgtcatca aatcgttgta gattcattac
    54901 tgctcctgaa cggtatacag gagtaagtgt agacgttggt aaatgatgtt tgtgaagtta
    54961 atccgctagc agggcaagat tacgaaagcg cgctccccgc aagcaactaa tcaaggtagt
    55021 taatcatggc tgaacacgat attacgccag aagtcaccat ttccaaaaca cagcgccgct
    55081 ataaagtcgg ctatgtcaga gcccgccacc aggaccgcaa aaccggtata gcccggtatt
    55141 atagcctgca ccttaagggc gactggctga aagaggccgg atttgatacc ggacggggtg
    55201 tcacggtgaa gatttcggag gggggcctga ccatcatcgc tgacagtgac gaggtgcagg
    55261 aactgcggga ggaactttat caggttaaac aggtagttaa ggggatgcgg gatgggatgt
    55321 ttagtgtgct gaacgaaagt taaatattaa aaaagctggg aagatttaaa accttcctag
    55381 ctttttattc tgtcttatta taatgtatac tcatcatctt ccgatgaatt tataaattgc
    55441 tccaagttta catgaagagt acttaaccta gtttttaact cattaagtga agcgataatt
    55501 tcatattttc caacataatc atatgatgga tcgtattcat tatctatgtc agaagctaaa
    55561 aatgatgata ttcgtagttt aaatttagct tcatctttat tacattcatt ggcataaggt
    55621 gaatgcccta tcatagcata ttcctctaat ctaaaaggat aagatggttt taattcatcg
    55681 atcaatgaaa tttcaatcca tgttctatat ccaggcatta atggactaat ttctttgagc
    55741 aaaacctttc cacttaataa tgtattga
//
LOCUS       DAJTYG010000016        55333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTYG010000016 DAJTYG010000000
VERSION     DAJTYG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.016"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..356
                     /locus_tag="OO568_001024"
     CDS             <1..356
                     /locus_tag="OO568_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR6640471.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="OO568_001025"
     CDS             complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="OO568_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6640472.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="OO568_001026"
     CDS             complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="OO568_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6640473.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLPFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFSNAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3111..3263
                     /gene="mokC"
                     /locus_tag="OO568_001027"
     CDS             3111..3263
                     /gene="mokC"
                     /locus_tag="OO568_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6640474.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="OO568_001028"
     CDS             complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="OO568_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6640475.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="OO568_001029"
     CDS             complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="OO568_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6640476.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRATD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSSRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6879..7283
                     /locus_tag="OO568_001030"
     CDS             6879..7283
                     /locus_tag="OO568_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6640477.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7309..8022
                     /gene="msyB"
                     /locus_tag="OO568_001031"
     CDS             7309..8022
                     /gene="msyB"
                     /locus_tag="OO568_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6640478.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8171..8737
                     /gene="satP"
                     /locus_tag="OO568_001032"
     CDS             8171..8737
                     /gene="satP"
                     /locus_tag="OO568_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6640479.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8772..9359)
                     /gene="mog"
                     /locus_tag="OO568_001033"
     CDS             complement(8772..9359)
                     /gene="mog"
                     /locus_tag="OO568_001033"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6640480.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9474..10427)
                     /gene="tal"
                     /locus_tag="OO568_001034"
     CDS             complement(9474..10427)
                     /gene="tal"
                     /locus_tag="OO568_001034"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6640481.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10706..12166
                     /locus_tag="OO568_001035"
                     /pseudo
     CDS             10706..12166
                     /locus_tag="OO568_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12236..13012
                     /gene="yaaA"
                     /locus_tag="OO568_001036"
     CDS             12236..13012
                     /gene="yaaA"
                     /locus_tag="OO568_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6640482.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13165..13461)
                     /locus_tag="OO568_001037"
     CDS             complement(13165..13461)
                     /locus_tag="OO568_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6640483.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13675..14961)
                     /gene="thrC"
                     /locus_tag="OO568_001038"
     CDS             complement(13675..14961)
                     /gene="thrC"
                     /locus_tag="OO568_001038"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6640484.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14962..15894)
                     /gene="thrB"
                     /locus_tag="OO568_001039"
     CDS             complement(14962..15894)
                     /gene="thrB"
                     /locus_tag="OO568_001039"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6640485.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15896..18358)
                     /gene="thrA"
                     /locus_tag="OO568_001040"
     CDS             complement(15896..18358)
                     /gene="thrA"
                     /locus_tag="OO568_001040"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6640486.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18384..18503)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18439..18504)
                     /gene="thrL"
                     /locus_tag="OO568_001041"
     CDS             complement(18439..18504)
                     /gene="thrL"
                     /locus_tag="OO568_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6640487.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18718..19404)
                     /locus_tag="OO568_001042"
     CDS             complement(18718..19404)
                     /locus_tag="OO568_001042"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6640488.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19804..19944)
                     /locus_tag="OO568_001043"
     CDS             complement(19804..19944)
                     /locus_tag="OO568_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640489.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20040..20756
                     /gene="arcA"
                     /locus_tag="OO568_001044"
     CDS             20040..20756
                     /gene="arcA"
                     /locus_tag="OO568_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6640490.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20815..22167)
                     /gene="creD"
                     /locus_tag="OO568_001045"
     CDS             complement(20815..22167)
                     /gene="creD"
                     /locus_tag="OO568_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6640491.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22225..23649)
                     /gene="creC"
                     /locus_tag="OO568_001046"
     CDS             complement(22225..23649)
                     /gene="creC"
                     /locus_tag="OO568_001046"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6640492.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNTIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRNFT"
     gene            complement(23649..24338)
                     /gene="creB"
                     /locus_tag="OO568_001047"
     CDS             complement(23649..24338)
                     /gene="creB"
                     /locus_tag="OO568_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6640493.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGVDDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24351..24824)
                     /gene="creA"
                     /locus_tag="OO568_001048"
     CDS             complement(24351..24824)
                     /gene="creA"
                     /locus_tag="OO568_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6640494.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25035..25904
                     /gene="robA"
                     /locus_tag="OO568_001049"
     CDS             25035..25904
                     /gene="robA"
                     /locus_tag="OO568_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6640495.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGKFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25901..26548)
                     /gene="gpmB"
                     /locus_tag="OO568_001050"
     CDS             complement(25901..26548)
                     /gene="gpmB"
                     /locus_tag="OO568_001050"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6640496.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26600..27121
                     /gene="yjjX"
                     /locus_tag="OO568_001051"
     CDS             26600..27121
                     /gene="yjjX"
                     /locus_tag="OO568_001051"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR6640497.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(27206..27532)
                     /gene="trpR"
                     /locus_tag="OO568_001052"
     CDS             complement(27206..27532)
                     /gene="trpR"
                     /locus_tag="OO568_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6640498.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27622..29559)
                     /gene="sltY"
                     /locus_tag="OO568_001053"
     CDS             complement(27622..29559)
                     /gene="sltY"
                     /locus_tag="OO568_001053"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6640499.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTHQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMLGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29770..31437
                     /gene="ettA"
                     /locus_tag="OO568_001054"
     CDS             29770..31437
                     /gene="ettA"
                     /locus_tag="OO568_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6640500.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLDQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGSQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31544..31711)
                     /locus_tag="OO568_001055"
     CDS             complement(31544..31711)
                     /locus_tag="OO568_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640501.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31745..32977)
                     /gene="nadR"
                     /locus_tag="OO568_001056"
     CDS             complement(31745..32977)
                     /gene="nadR"
                     /locus_tag="OO568_001056"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6640502.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32998..34380)
                     /gene="radA"
                     /locus_tag="OO568_001057"
     CDS             complement(32998..34380)
                     /gene="radA"
                     /locus_tag="OO568_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6640503.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34429..35397)
                     /gene="serB"
                     /locus_tag="OO568_001058"
     CDS             complement(34429..35397)
                     /gene="serB"
                     /locus_tag="OO568_001058"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6640504.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAARSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVLANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35503..36147
                     /locus_tag="OO568_001059"
     CDS             35503..36147
                     /locus_tag="OO568_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6640505.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTQLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36175..37191
                     /gene="lplA"
                     /locus_tag="OO568_001060"
                     /pseudo
     CDS             36175..37191
                     /gene="lplA"
                     /locus_tag="OO568_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37223..37486
                     /locus_tag="OO568_001061"
     CDS             37223..37486
                     /locus_tag="OO568_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6640506.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37647..38366)
                     /gene="deoD"
                     /locus_tag="OO568_001062"
     CDS             complement(37647..38366)
                     /gene="deoD"
                     /locus_tag="OO568_001062"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6640507.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38423..39646)
                     /gene="deoB"
                     /locus_tag="OO568_001063"
     CDS             complement(38423..39646)
                     /gene="deoB"
                     /locus_tag="OO568_001063"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6640508.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLIRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39698..41020)
                     /gene="deoA"
                     /locus_tag="OO568_001064"
     CDS             complement(39698..41020)
                     /gene="deoA"
                     /locus_tag="OO568_001064"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6640509.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MVLCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41147..41926)
                     /gene="deoC"
                     /locus_tag="OO568_001065"
     CDS             complement(41147..41926)
                     /gene="deoC"
                     /locus_tag="OO568_001065"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6640510.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRYMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42184..43725
                     /locus_tag="OO568_001066"
     CDS             42184..43725
                     /locus_tag="OO568_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6640511.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43697..44560
                     /locus_tag="OO568_001067"
     CDS             43697..44560
                     /locus_tag="OO568_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6640512.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEVTTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44774..45556)
                     /locus_tag="OO568_001068"
     CDS             complement(44774..45556)
                     /locus_tag="OO568_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6640513.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45553..46626)
                     /locus_tag="OO568_001069"
     CDS             complement(45553..46626)
                     /locus_tag="OO568_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6640514.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGGSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46748..46909)
                     /locus_tag="OO568_001070"
     CDS             complement(46748..46909)
                     /locus_tag="OO568_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6640515.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(47036..47641)
                     /gene="osmY"
                     /locus_tag="OO568_001071"
     CDS             complement(47036..47641)
                     /gene="osmY"
                     /locus_tag="OO568_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6640516.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKDYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(48034..49623)
                     /gene="prfC"
                     /locus_tag="OO568_001072"
     CDS             complement(48034..49623)
                     /gene="prfC"
                     /locus_tag="OO568_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6640517.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49714..50391)
                     /gene="yjjG"
                     /locus_tag="OO568_001073"
     CDS             complement(49714..50391)
                     /gene="yjjG"
                     /locus_tag="OO568_001073"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6640518.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50406..50852)
                     /gene="rimI"
                     /locus_tag="OO568_001074"
     CDS             complement(50406..50852)
                     /gene="rimI"
                     /locus_tag="OO568_001074"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6640519.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50821..51234)
                     /gene="holD"
                     /locus_tag="OO568_001075"
     CDS             complement(50821..51234)
                     /gene="holD"
                     /locus_tag="OO568_001075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6640520.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51337..52368
                     /gene="rsmC"
                     /locus_tag="OO568_001076"
     CDS             51337..52368
                     /gene="rsmC"
                     /locus_tag="OO568_001076"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6640521.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRT
                     IMTRQAKKG"
     gene            52567..52653
                     /locus_tag="OO568_001077"
     tRNA            52567..52653
                     /locus_tag="OO568_001077"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52601..52603,aa:Leu,seq:cag)
     gene            52682..52768
                     /locus_tag="OO568_001078"
     tRNA            52682..52768
                     /locus_tag="OO568_001078"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52716..52718,aa:Leu,seq:cag)
     gene            52803..52889
                     /locus_tag="OO568_001079"
     tRNA            52803..52889
                     /locus_tag="OO568_001079"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52837..52839,aa:Leu,seq:cag)
     gene            complement(52928..53164)
                     /locus_tag="OO568_001080"
     CDS             complement(52928..53164)
                     /locus_tag="OO568_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6640522.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            53305..54093
                     /gene="fhuF"
                     /locus_tag="OO568_001081"
     CDS             53305..54093
                     /gene="fhuF"
                     /locus_tag="OO568_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6640523.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(54131..54808)
                     /gene="bglJ"
                     /locus_tag="OO568_001082"
     CDS             complement(54131..54808)
                     /gene="bglJ"
                     /locus_tag="OO568_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6640524.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(54766..>55333)
                     /locus_tag="OO568_001083"
     CDS             complement(54766..>55333)
                     /locus_tag="OO568_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6640525.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        13256 a        14915 c        13815 g        13347 t
ORIGIN      
        1 attatcagtt tcatcatata agatttgact gcatccaggg cgagcaaggt gaattagttt
       61 ttgaacccag tgacgttgaa tattactttg aaccagtgac gatacaggag tcaggaacca
      121 gcatattaaa aaatgagtta gaaaacagtg aggacggtgc gggtcaaata ggctttcaac
      181 tctccaatga tggtacgaat gaaatccagt atggtaaaag caattactac tcttttcagc
      241 atccccatga aggaagtaat cagattcctc tatttattcg cccgcgtaca tatggaaaca
      301 atgtctcaag cgggcaaata atgagccgag tcaaaatcgt ggtaatgtac aactaatcta
      361 tctcccccac cttaactttg ggggagctgc cgattaacgc accgctggac taaaaagcgc
      421 agaataatcc gtattgcaga ttcgctgtac cgccgggtgc tgaatcatcc gctcagcaaa
      481 aatagcatgg tactcttcca tcacattctc gacgcgacca atttctacga cagttttatc
      541 ggcataaaaa tcatatgcat aaagcgttgg ggcaacgaag attgcattgt gcatcgcacc
      601 aaaagctttc atcaaagcgg catcatcaaa ctcgccgagg atttctacgt ttaatccctg
      661 ggagttaaac cagttaagca atttgcgccc taacattgaa cgtcgcccag gaatcaaaag
      721 tcgccgttct tccagacaag ccgggaacgg tttttctggt ggtggatttg tacaccagaa
      781 actcacgcca cattcgccaa ttctcacgga gaacaggcct tcctgctgcg tagagtctat
      841 cggacagtca gaaatgatca tatccagttt atgctgactt aattgctcca gcagcatttc
      901 gtgggtggat tcgaagcagc gaagatgaat gggctcgcct tctaccactg cggcgttaag
      961 tacgctactg accaggcgtt tggaaagtgc atcagccacg ccaacgtcaa acaataaatt
     1021 ggattctttg cgatagttca caatatccag catttcctgg cttaaggtga acattttatc
     1081 ggcatagcga tagaccagtt ctcccagctc gctgggttcg agaccacgtc ccttgcgttt
     1141 aaataatttg ccttgcaggc gctcttccag cgctcgaatc tgtccggtaa tggtttgtgg
     1201 agttaaataa agcgcctccg ctgcgccaac cacggaacct tctttataga catgccagaa
     1261 gtaatacaag tggttgtaat tgatatgaga catgctcatt tctctccctg ataacaatga
     1321 aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc aaactgatgg acgcaaacga
     1381 acgcgtaacc agctgtatcc aattaccgcc gaagagatag aaccgaccag gataccgagt
     1441 ttcgcccagt taatcagttc tggatctacg ctaccaaagg ccaggctggc aataaagata
     1501 gacatagtaa aaccgatacc gcacaggatc cccaccacca taatttgctg ataagtcgtt
     1561 ccctcaggca gatgcgccag tttcaaacgc agcgccaacc agcagaacag actaatcccc
     1621 agcggtttgc caatcagcaa gccagcgatg atccccaatg gcagaatgga ggtcaagcca
     1681 tccagcgtga cgccttgcag tgaaacgcca gcattagaaa atgcaaacag cggcaaaatc
     1741 agatacgcca cccacgggtg caacacatgc tccagtcgct tcgctggaga acgcccatgc
     1801 ttctctttca aaggaataaa gaagccgaca attacccccg ccagagttgc gtgaaccccc
     1861 gatttcaaca ccgcagtcca caacaccacg ccaacaagaa tatagacgcc cgtgcggcgt
     1921 acaccacaca gattcaatac cgcgagtacc gcaattgcta cagccgcgac gccaagagag
     1981 gccatcgata agtcattagt gtagaacaat gcgatgataa tgatggcccc aagatcgtcg
     2041 ataatagcca gagccatcaa aaagatcttc agcgctaacg gaacacgact tcccaacagc
     2101 gccagtacac caagtgcaaa agcaatgtca gtagccgccg ggatcgccca cccttcgcgg
     2161 gtaatcggat cggcatagtt aaaaggcaga tagagtaatg ccggcacaat catcccacca
     2221 atagcggcga taactggaaa tgcggcctgg cgtaagctgg ctagcgatcc ttgcatcagt
     2281 tcacgtttaa cttccagacc gactaacagg aaaaataccg ccatcagcgc gtcatttatc
     2341 cataacagca tgtttttgtt gatttcgagt gaaccaaccc ggagctgaac cggcgtctcc
     2401 agaaagtcgt gataccatcc actggttgcg ccgctgttgg ccataatcat cgccaggata
     2461 gcggcaatga taagtataat gcctcccgag gcatcactgc taaagaatcg atgcagatgt
     2521 ttcacttttt atttctcttt caggtgaata gatcgttttt actattttac cccgcacgaa
     2581 tcatcgttaa aatcagatta tagtgtagga ataattcgtt ttttacgagc tattgaagaa
     2641 tttgcgccat cagcggtgag atgcgttttt gacggctgta agcgctcacg agtgacccta
     2701 agcccagata atcatagctt aaggtgagga ttcctgaggg attaattagg aaaagtaata
     2761 attaatttgt tgtcagtcgg cgatgcttgt taggccaata tttcaaaaat aatccagctt
     2821 gtcgtcaata attgcgatga agagataaat taaagttaaa taattaaatc attaaaaaat
     2881 tacgccacaa aaatgcgagg cgtcttcagg attagaatcg ataaaacaaa atgggttaaa
     2941 gagagcgata taatagcggc gggtgcctga ggctgtctgt ctcaggcatt agctgaacgg
     3001 cagatagaga aaagccccga gtgatatttt accatcaacc cgaggcctcc tatatgctga
     3061 acacatgtag agtgcctctt actgaccgta aggtcaagga gaagagagca atgaagcagc
     3121 ataaggcgat gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg gcggcgctgg
     3181 tgacgagaaa agacctctgt gaggttcaca tccgaactgg ccagacggag gttgctgttt
     3241 tcacggctta cgaatccgag taagagcaac ggcggggagt gatccccgcc actttacagg
     3301 tgctcgcata tcttcaacgc accctatttt tacccaggcc tgcccacggg caggcttttg
     3361 gggaggttag cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga
     3421 gcgcgggctg ttgtgctcgc cggttgggcc accgaagctt tcttgcagct cttgcagcag
     3481 ctgtttctgc ttttcgttca ggcctaccgg tgtttcgacg acaacgcggc acagcaaatc
     3541 accctgtgca ccaccgcgga cagacttgac gcctttaccg cgcatacgga atagcttacc
     3601 ggtctgggtt tcgccaggca ctttcagttt gacgcgacca tcaagggtcg gtacttcgat
     3661 ttcgccaccc agcgccgcca tagcgaagtt gatcgggact tcgcaataca ggttgttgcc
     3721 ttcacgctcg aaaatcgggt gctgtttaac ctgaacctga acgtacagat cgcctgccgg
     3781 tgcgccatgc tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc cagtgtccac
     3841 ccctgccggg attttaacgg acagcgtttt gctgcgctca acacgaccat gaccatgaca
     3901 tttgttgcac ggatctttga tcagcgtacc gcggccctga cagtgtggac aggtctgctg
     3961 tacagcgaag aatccctggc gcatctgcac ctgaccagaa ccatgacagg tcggacaggt
     4021 ctgcggctgt gtacctggtt ttgcaccgct accgtggcaa acgtcacact cttccagagt
     4081 cggaatgcgg atttctttgg tcacgccacg tacagcttct tcgagggtga gctccatgtt
     4141 atagcgtaaa tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc
     4201 gccgaaaacg tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac caccgccgcc
     4261 catgcctcct tgctcaaacg cagcatgacc atactgatcg tatgccgcac gtttttgcga
     4321 gtcggtcaga acttcataag cttccttgat ctctttaaat ttcgcctcgg cctctttgtc
     4381 accctggtta cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct ttctgatttc
     4441 acgctcttcc gctgttttgg aaacgcctaa aatctcgtaa taatcttgct tagccatctt
     4501 ttttaaattg cccctagatg aatgcacggg cggagaggaa attccccttc gcccgtgtca
     4561 gtataattac ccgtttatag ggcgattatt ttttgtcttt gacttcttca aattcagcgt
     4621 cgacaacatc gtcatctttc gcgttgtttg cagaagcatc agcaccggca gtctgctgct
     4681 gggcatgttg ctgctgggcg atttccatca gtttctggga aacctgtgcc agttcctgca
     4741 ttttcgcttc gatagcggct ttgtcttcac ctttcagagc agtttccagt gcagtcagcg
     4801 cagactcgat agcagttttg tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct
     4861 tacgggagct gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact
     4921 tacggtcagc ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt tcatcttcgt
     4981 tcagaccaga agaagccttg atggtgatct tctgctcttt accgctgttt ttatctttcg
     5041 cggaaacgtg caggataccg tcagcatcga tatcgaaggt aacttcgatc tgcggcatgc
     5101 cgcgcggtgc cgggttgata ccatctaggt tgaactgacc cagagatttg ttatcagtcg
     5161 cacgtttacg ttcaccctgc agcacatgga tggttaccgc agactggttg tcttcagcgg
     5221 tagagaacac ctggctgtgc ttggtcggga tagtggtgtt tttcgcgatc agcgtcgtca
     5281 tcacaccgcc catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt
     5341 cttttacgtc accagtcaga acaccaccct gaacagcagc accgattgct acagcttcgt
     5401 ccgggttaac gtctttacgc ggctctttac caaagaactc agcaactttc ttctgaacca
     5461 ttggcatacg agtctgacca ccaacgagga taacgtcgtc gatatcagat acggacaggc
     5521 cagcatcctg cagtgcaact ttcagcggct caatggaacg gtttaccaga tcttcaacca
     5581 ggctttccag tttcgcacga gtcactttga tgttcatgtg tttcggaccg gtcgcgtctg
     5641 cagtgatgta tggcaggtta acgtcggtct gctgagcgga agacagttcg attttcgctt
     5701 tttctgccgc ttctttcagg cgctgcattg ccagcggatc gttgcgcagg tcaatgccct
     5761 gatctttctt gaattcttca accagatagt tgatcagacg gctgtcgaag tcttcaccac
     5821 ccaggtgggt atcaccgttg gttgccagaa cttcgaaggt tttttcgccg tcaacttcgt
     5881 cgatttcgat aatagaaata tcgaaagtac caccccccag gtcataaacc gcgatagtac
     5941 ggttgccagt gcctttgtcc agaccgtaag ccagcgcagc tgcggtcggt tcgttgatga
     6001 tacgttttac ttccagacca gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat
     6061 cgttaaagta tgccggtacg gtgataacag cttcagttac cggttcaccc aggtaatctt
     6121 cagcggtttt cttcattttt ttcagcactt cagcagaaat ctgcggcggt gccattttct
     6181 ggcctttaac ttcgacccat gcgtcgccgt tatcagcagc aataattttg aacggcatga
     6241 tggaaacatc acgctgtact tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg
     6301 caaacagggt gttttgcggg ttcgtcactg cctgacgttt agccggctga ccaaccagag
     6361 tttcaccatc ctgggtatag gcaatgatag aaggcgtggt gcgatcgcct tcggcgttct
     6421 ccagcacgcg aggaatggtg ccatccataa tcgctacaca agagttggta gtacccaggt
     6481 cgataccaat tattttaccc atctaaacgt ctccactata tattcggtca tcatgtggtt
     6541 gtgagtctgt aataggggcg aaacgtctgg tttcaactgc ccaatttcat ttttttgcag
     6601 actcactcac tgcggttgac tactaaatgg ggtcgtaacc cacgtcatca agggggagat
     6661 gcaaaaattt tttgtgcttt tgcgcagaaa tttcgcgata tggcaggaca agacgtaaaa
     6721 ttgacagcaa ttttattcat ataaagaatg aatcgcagcc aaggcattca tcaaaaaatt
     6781 gtaataaaaa gaaaagatta cgtgcctgaa tcttctcttt atcagcagta aactagtggg
     6841 tattcatccc cctacctctt cccactaaga gaatccttat gaaatccgtt tttacgattt
     6901 ccgccagcct ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaac
     6961 tcctcggcgt cattgcaatg ccgcgtaacg aaaccaacga tctggcgctg aaacttcctg
     7021 tttgtcgcat tgtgaaacgc atacaactct ctgccgacca tggcgattta cagttaagcg
     7081 gtgcatcggt ttatttcaaa gccgcccgta gcgccagtca gagcctgaat attccttcag
     7141 aaataaaaga agggcaaacc actgactgga tcaacattaa cagcgataac gacaataaac
     7201 gctgcgtctc aaaaatcacc ttttccggtc atacggtgaa ctcatcggat atggccacgc
     7261 tgaaaattat cggcgacgac taacgccaga atatagaagc cacaaaaaat gaatgttaat
     7321 tacctgaatg attcagatct ggattttctt cagcattgta gtgaggaaca gttggcaaat
     7381 ttcgcccgat tgctcaccca taatgaaaaa ggcaaaactc gcctctccag cgtactgatg
     7441 cgcaacgaac tgtttaaatc gatggaaggg catcccgagc aacatcgccg caactggcag
     7501 ctgattgccg gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga
     7561 cacggtaaat tgtatcgggc cattttgctc gatgtttcaa agcgattgaa gctgaaagcc
     7621 gacaaagaga tgtctacgtt tgaaattgag caacagttac tggaacaatt tctgcgtaat
     7681 acctggaaga aaatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg
     7741 gtgaatgagc tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggt
     7801 gtgtcgcatt tgctttccag ccaactgacc cgcattttac gcacccacgc agcaatgagc
     7861 gtacttgggc atggtttgct gcgcggagcg gggctgggag gccctgtggg cgcagcatta
     7921 aatggggtta aagcggtcag cggcagcgcc tatcgcgtga cgattccagc cgtactgcaa
     7981 atcgcctgcc tgcgccggat ggttagcgcc actcaggtct gataccggaa accggggaaa
     8041 attttcattt tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc
     8101 gccctgaaaa cactacagtt attcagggaa attatttcac cattcattcg atgatgattt
     8161 ttgaggaatt atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt
     8221 cggcatgacc accattctgc ttaacctgca caacgtgggt tatttcgctc tggacggtat
     8281 tattcttgcc atgggcattt tctacggcgg catcgcgcaa atttttgctg gtctgctgga
     8341 gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct
     8401 gacgctggtt gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca
     8461 gttccttggt gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac
     8521 tctgaaaggc gcacgcgtcc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct
     8581 gctggcgatc ggtaacattg ccggtaatgc cgcgatcatc cactttgccg gctggattgg
     8641 tctgatctgc ggtgccagcg caatctatct ggcgatgggt gaagtgctga acgagcagtt
     8701 tggtcgcacc gttctgccga ttggtgaatc ccactaattg tcttgagatc cccccgctcg
     8761 gggggatttt tttattcgct aacgtcgcgt cttgcactct tcggtctgaa tgcagcaacc
     8821 acttctggtg ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg
     8881 gcaaaaatac catgcaccac aacgttaccc tcagcgtcct tcacaccttc cagcgtctct
     8941 ttaatagact tcggctgacc gggtaagtta aggatcagcg cctgtttacg aatcacgccc
     9001 acctgacgcg aaaggatcgc agttggtaca aaatgcaggc tgatctggcg catctgttca
     9061 ccaaagccag gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga
     9121 cgcgccgggc cagttccgcc cgtggtgagc accagatggc aactcatttc atccaccagc
     9181 tcacacaacg tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt ttccagttca
     9241 aacggcgtgg ttagcgccga tgtcagccat tcttccagcg ctggaatgcc tttatcctga
     9301 taaacgccgc tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc
     9361 aagatttttc cgtttaaaca gtctcgttaa acagaatgat acactgcgaa gggagtgaca
     9421 gacaggcttc agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc
     9481 agatcgccga tcattttttc cagtttttcc tggtcaatag caaacttacg gataccttcc
     9541 gccagtttat ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg
     9601 atacgcgccg gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc gatagcccct
     9661 tcgctctccg ccagctcttt cagcagtgcc ggtgcgatgg tcagacggtc acagcctgcc
     9721 agttccagaa tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg
     9781 tgctctttgt agtactggta gatttcagat acagaaacca cgcccggatc ttctgccgga
     9841 gcgtactctt tcttatcggt attcgctttg taccagtcaa gaatacggcc aacaaacggc
     9901 gagatcagga acacgcccgc ctccgcacaa gcacgagcct gagcgaagga gaacagcagg
     9961 gtcaggttac agttgatacc ttctttttcc agctgttctg cagcacggat accctgccag
    10021 gtagaagcca gtttgatcag aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg
    10081 atcaggcgtt ttgctttcgc aattgacgct tcggtgtcat aggaaagacg cgcatcaact
    10141 tcagttgaga tacggcccgg aaccagtttc aggatttcca gaccaatatt tactgccagt
    10201 ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg
    10261 acagcatcat caatcaactt acggtattcc ggaatctgcg ctgcgttaag aatgagagaa
    10321 gggttggttg tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg
    10381 gccactacgg tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc
    10441 tttaaacagc ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt
    10501 cagcgcaacc gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact
    10561 gcgttgatat cgcccgccat ttttatacaa aacctcatgt atgctacgca gaagttatca
    10621 agtacctcgt agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc
    10681 gcgaccggca ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct
    10741 ttggggatcg gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac
    10801 cggatttgtg cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca
    10861 tccacagcca ggcggtttaa cctcatttca gtcattgtgt accagtcttg cggcgcgcgt
    10921 gggtagcggc aacctggccg ccgttgcgct ggcaattacc gccggtggac caggtgccgt
    10981 cttttggatg tgggttgctg cgtttatcgg catggcgacc tcgtttgccg aatgttccct
    11041 tgcacaactt tataaagaac gtgacgtcaa tgggcagttt cgtggcggac cggcatggta
    11101 tatggcgcgc gggctgggga tgcgctggat gggcgttctg ttcgccgtct ttttgctcat
    11161 cgcctacggc ataattttca gcggagttca ggcgaacgcc gttgcccgag ccctgagttt
    11221 ttcttttgat tttcctccgc tggtgacggg cattattctc gctgtctttg ctctgctggc
    11281 aatcactcgc ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc
    11341 gataatctgg gtactgacca gcctggtgat ttgcgtaata aatatcgggc aacttcccca
    11401 cgtcatttgg tctatttttg agagtgcttt tggctggcag gaagcggcag gcggcgcggc
    11461 gggatatacc ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga
    11521 ggcgggaatg ggatcgacgc caaacgcggc agcggcagcg gcagcggcag cggcagcggc
    11581 agcggcagct gcatcctggc ctccgcatcc ggcagcacaa gggattgtcc agatgattgg
    11641 catttttatc gacaccctgg tcatctgtac ggcaagcgcc atgctgatat tactggcggg
    11701 taacggcaca acctacatgc cgctggaagg tattcagctt atccagaagg cgatgcgggt
    11761 gctaatgggt tcctagggtg ctgaatttgt taccctcgtg gttattctgt ttgccttcag
    11821 ctccatcgtt gccaactaca tttatgccga aaacaatctc ttctttttac gcctgaacaa
    11881 ccctaaagcg atctggtgtt tgcagatctg caccttcgca acggtcatcg gcggcacctt
    11941 gctaagcctt ccgctgatgt ggcaactggc agatatcata atggcctgca tggctattac
    12001 caatttgacc gccattttac tgctctcgcc tgtggttcat accattgcca gtgattatct
    12061 acgccagcgt aaactcggcg tgcgcccggt gtttgatccg ttgcgttatc cggatatcgg
    12121 tcgccagctt tctccggacg cgtgggatga tgtttcgcag gagtaatcac aactatcgat
    12181 caactcattc tctttttttg ctaaagtcgg cataaatttc ctgcaaggac tggatatgct
    12241 gattcttatt tcacctgcga aaacgcttga ttaccaaagc ccgttgacca ccacgcgcta
    12301 tacgctgccg gagctgttag acaattccca gcagttgatc catgaagcgc ggaaactgac
    12361 gcctccgcag attagcacgc tgatgcgcat cagcgacaaa ctggcgggta tcaacgccgc
    12421 tcgctttcat gactggcagc cagatttcac gccggcgaat gcccgccagg cgattctggc
    12481 gtttaaaggt gatgtctaca ccggcttgca ggccgaaacc ttcagcgaag acgatttcga
    12541 ttttgcccaa cagcatttgc gaatgctttc cggcttgtat ggcgtactgc gcccgctcga
    12601 tttaatgcag ccttatcgtc tggaaatggg gatccgtctt gagaatgccc gagggaaaga
    12661 tctgtatcaa ttctggggag atatcatcac caacaagctg aacgaggcgc tcgcagcaca
    12721 aggcgataat gtggtgatta atctggcgtc agatgagtat tttaaatcgg tgaagccgaa
    12781 gaaattgaat gccgagatta tcaagccggt gttcctcgat gagaagaacg gcaaatttaa
    12841 gatcatcagc ttctacgcta agaaagcacg cggtctgatg agtcgtttca ttattgaaaa
    12901 tcggctgacc aaaccagagc aactgactgg ttttaatagc gaaggttact tctttgatga
    12961 agattcctcc agcaatggcg aactggtgtt taaacgctac gagcagcgtt aagtttgtag
    13021 tcaatgccgg atgcggcgtg aacgccttat ccggcctaca aaagcatgca gattcaatat
    13081 attgcagaat taacgtaggc ctgataagac gcgccagcgt cgcatcaggc gctgaatgcc
    13141 ggattgttac ccggcatttg tcatttagcg gtgatgtttg cctggaccat gaccgccgtg
    13201 atgatcatga ggagctttct tatggtggcg cggcggtggc ggcggtccgt gtgggtgcca
    13261 gcgattgcct cgccattcat aatgttgttt ccaccagccg tggtcgcgcc agtgacctcc
    13321 atcccagtaa tagccacgat tatcacgatc gcctatctgt aattttactg acgggactaa
    13381 cgtaatttcc gcagcctgtg ctgccatggg agcgaccaga accagggaaa gtgcgagtac
    13441 gatagattgc atctttttca caggttactc cttttatcat tgcccgttat gggtcgatat
    13501 ccggagatta cgcgatgaga acgacggttg ttattctccg caatcctaat ctctaagtta
    13561 ccgcatttat tgataatttc tcctttttcc ctgtcgtttt tctccataat ttcttcataa
    13621 aaaagccggg ctgcataaaa gcaaacccgg cctgattgag ataatgaata gattttactg
    13681 atgattcatc atcaatttac gcaacgcaga aaaatcggcg ggcagattat gtgaaagcaa
    13741 gggtaaatca gcacgttctg ccagctcttt tggcagatcc aacgtttcac cgagaatcgc
    13801 ttccacgctc tctttaaatt tcgccggatg cgcggtgccg aggaacaagc catattcgcc
    13861 tggattcaac tggtcacgca gcgcacgata cgctacggca gcgtgcggct ccgaggtgta
    13921 gcccagttct tttaactcac gcattgtctg ttgcgtggtt tcatcatcca cggctgcata
    13981 acccagctct ttcagttgcc agattttgcg gcggaacaac tcttccacac gcggccagtt
    14041 gttcggctga ctcacgtcca tcgcgttgga taacgtcgcc tgagtcgctt tgggtgacca
    14101 ctgaccgtcg tgcaggaaac gtggcacggt atcgttcacg ttggtcgcag caataaaacg
    14161 tttcaccggc agaccgaatg acttcgccag cagacccgcc gtcaaatcgc cgaagtttcc
    14221 gcttggcacc gagacaacca gctggttgcg cgcctcctgc ggcagctgcg caacagcttc
    14281 aaagtagtag caaatctgcg ccagcaaacg gctgatgtta atcgagttag ccgagtttaa
    14341 ccccagcgcc actttcagtt cttcatcatc aaacgcctgc ttcaccagcg cctgacaggc
    14401 atcgaaatcg ccgtcgatgg caacagtttc gatattgccg cccaacgtac agaacagttt
    14461 ttcttgcagt ggactgattt tgcctcgtgg atagaggata accactttca cattcggcaa
    14521 accgtagaaa gcatgagcca ctgcagctcc ggtatcaccg gaggtcgcgg tcagaatggt
    14581 cactggctta tcatccgcaa tatgggtcag catttgtgcc ataaagcgac cgccgaaatc
    14641 tttaaatgcc agcgttggcc cgtggaacaa ttccagacaa ccgacatcgc tttcaacatt
    14701 ggcgaccgga gccgggaagg caaacgccgc gcgcacgcgc tcttccagga tttcctgtgg
    14761 gatttcatca ccaataaacg ccgagaggat cttcgcactg cgggtgacaa aatccagctt
    14821 cagcatctca tcaatttcag tcaggctgaa ttccggcagg tcgtgcggaa aaaacagccc
    14881 ctgatttttg cccaacccct gggttacggc ttgcgcaaag ctgacctgct cgttgtgatc
    14941 tttcagattg tagagtttca tttagttttc cagtactcgt gcgcccgccg tatccagccg
    15001 gcaaatatga acaaaacctt cctgattttg caggtagttc ttacccaacc agtcggcaac
    15061 gcgctgggcg gtatccggct tgtcacatag agcgaacaag gtcgggccgg agccggagat
    15121 accgctcgct accgcgccga tttccgcgac cgcctgccgc gcctgccgga agccaggcag
    15181 taaccgttca cggtagggtt cagcgataac atctttcatc agcttcgcgg caagctcagg
    15241 ctgacgggaa tagcaggcgt gaatgaagcc agccagatgt cgcccgtgcg caatgcaatc
    15301 ctggcggcga tactgcgccg gtaaaatagc ccgggcttct gccgtcgaga ctttaattcc
    15361 cggatacgcc agcacccaca gccactcatc aaaccctggc acttgctggc tgatgatgtc
    15421 gttttcttcg atcatcaact gcataccacc aagaaaacac ggtgccacgt tgtcgtaatg
    15481 aatgctgccg gagatacgtc cttccagctc gcccatcaaa gccagcaaac gagtgtcatt
    15541 aagcggcttg ccgcagtgtt cattcatcgc catcagcgcc gcgaccaccg aacaggcgct
    15601 ggagcctaag cccgaaccga tcggcatatt cttttccaga gtcatcgcca ctggaatttg
    15661 cttgccaagc tcctggcaaa aacgctccca gcactgataa acgatatttt cccgtggttc
    15721 tgacggcagc ttatcggcaa agcgtccgag gttgttgaga ctgaatgtct ctgccgcctc
    15781 aaccgtgact acatctccga gcaatgcacc atcaacgggt gccaccgccg ccccgagcac
    15841 atcaaacccg acgctcatat tggcactgga agccggggca taaactttaa ccatgtcaga
    15901 ctcctaactt ccatgagagg gtacgtagca gatcggcaaa gacaccggca gcggtaacgt
    15961 cattgcccgc accatatccg cgcagcacca acggcagcgg ctgataatag tggctataaa
    16021 aggccagggc gttttcgcca tttttcactt tgaacagcgg atcattccca tccacttcgg
    16081 caatcttcac gcggcagaca ccatcttcat caatattgcc aacatagcgc aaaacttttc
    16141 cttcatcacg ggccttcgcc acgcgcgcgg caaagagatc gtcgagctgt gacagattcg
    16201 ccataaaagc ggcaacatca ccctcggcgt taaactctgc gggcagcaca ggttcaattt
    16261 caatatccgc cagctccagt tcacgtcccg tttcacgagc gagaatcaat agtttacgcg
    16321 ccacatccat accagaaaga tcatctcgcg ggtccggttc ggtataaccc atttcccgcg
    16381 ccagcgtggt cgcctcggag aaactcatgc cttcgtctaa cttgccgaag atataagaaa
    16441 gcgaacctga aagaatgccg gagaacttca ccaattcatc acctgcattg agcagatttt
    16501 gcaggttctc aataaccggt aatccagccc caacgttggt gtcatagagg aatttacgcc
    16561 gcgatttttc cgccgcataa cgcaactgat gatagtaatc catcgacgag gtgttggcct
    16621 ttttgttcgg cgtgacaacg tggaaacctt cgcgcaggaa gtcggcatat tgatccgcca
    16681 ctgcctggct ggaggtgcag tcaacaatga ccgggttcag cagatgatat tctttcacga
    16741 ggcgaattaa gcgcccgaga ttaaacggct ctttggcttg tgcaagttct tcctgccagt
    16801 tttccagatt aaggccatgc acactagtga gcagagcctt cgagttggca acgccgcaga
    16861 cacgtaagtc gatatgttta ttcttcagcc agctttgctg acgcttcagt tgctccagca
    16921 gcgcaccgcc aacgccaccg acgccaatca caaacacttc gataacctga tcggtattga
    16981 acagcatctg atgagtaacg cgcacgccag tggtcgcatc atcgttattt accacgacag
    17041 agattgagcg ttcagaagat ccctgagcaa tggcgacaat gttgatattg gcgcgggcca
    17101 gcgcggcaaa gaatttcgcc gagatcccac gcaaggtgcg cataccatca cctacaaccg
    17161 agataatggc cagccgttcc gtcaccgcca gcggctccag taagccttct ttcagttcca
    17221 ggtagaactc ttcctgcatt gcccgttcag ctcgcacaca gtcgctttgt ggaacgcaga
    17281 aactgatact gtattcggaa gatgattgcg taatcagcac cacggaaata cgggcgcgtg
    17341 acattgcagc aaagacgcgc gccgccatgc caaccatccc tttcatcccc gggccggaaa
    17401 cgctgaacat tgccatgtta ttcagattgg aaatgccctt gaccggtaat tcgtcttcat
    17461 cacggctggc accaatgagc gtacctggtg cttgaggatt tccggtattt ttaatcaggc
    17521 aagggatctg gaactgggcg atgggggtaa tggtgcgggg gtgaagaact ttagcgccga
    17581 agtaggaaag ctccatcgct tcctggtagg acatcgactt caacaacctc gcatcgggca
    17641 cctgacgcgg gtcgcaggta tagaccccgt caacgtccgt ccaaatctcg caacaatcgg
    17701 cgcgtaaaca ggcagccagc accgccgcgg agtagtcgga accgttacgt ccaagcacca
    17761 ccagttcgcc tttttcatta ccggcggtga aacctgccat caacaccatg tgatcagccg
    17821 gaatgcggct tgccgcaata cggcgggtgg actcagcaat atcgacggta gattcgaggt
    17881 aatgccccac tgccagcagt ttttcgaccg gatcgataac ggtaacgttg tgaccacgcg
    17941 cttctaacac gccggccata atggcgatcg acattttctc gccacggcaa atcagcgcag
    18001 cgttgatgct atccgggcac tgccccaaca aactaatgcc atgcaggaca tgttttattt
    18061 gggcaaattc ctgatcgacg aaagttttca attgcgccag cgggaacccc ggctgggcgg
    18121 cggcgagtcc cgtcaaaagt tcggcaaaaa tacgttcggc atcgctgata ttgggtaaag
    18181 catcctggcc gctaatggtt ttttcaatca tcgccaccag gtggttggtg attttggcgg
    18241 gggcagagag gacggtggcc acctgcccct gcctggcatt gctttccaga atatcggcaa
    18301 cacgcagaaa acgttctgca tttgccactg atgtaccgcc gaacttcaac actcgcatgg
    18361 ttgttacctc gttacctttg gtcgaaaaaa aagcccgcac tgtcaggtgc gggctttttt
    18421 ctgtgtttcc tgtacgcgtc agcccgcacc gttacctgtg gtaatggtga tggtggtggt
    18481 aatggtggtg ctaatgcgtt tcatggatgt tgtgtactct gtaattttta tctgtctgtg
    18541 cgctatgcct atattggtta aagtatttag tgacctaagt caataaaatt ttaatttact
    18601 cacggcaggt aaccagttca gaagctgcta tcagacactc tttttttaat ccacacagag
    18661 acatattgcc cgttgcagtc agaatgaaaa gctgaaaaat acttactaag gcgtttttta
    18721 tttggtgata tttttttcaa tatcatgcag caaacggtgc aacattgccg tgtctcgttg
    18781 ctctaaaagc cccaggcgtt gctgtaacca gtcgaccagt tttatgtcat ctgccactgc
    18841 cagagtcgtc agcaatgcca tgactcgttc gcgtaaagct tgcagttgat gttggtctgc
    18901 cgttgtgtca cttttcgccg gttgttgtat taatgttgct aattgatagc aatagaccat
    18961 caccgcctgc cccagattga gcgaaggata atccgccacc atcggcacac cagtaagcac
    19021 gtcagccaac gctaactctt cgttagtcag cccggaatct tcgcgaccaa acaccagcgc
    19081 ggcatggctc atccatgaag atttttcctc taacagcggc accagttcta ctggcgtggc
    19141 gtagtaatga tatttcgccc gactacgcgc agtggtggcg acagtgaaat cgacatcgtg
    19201 taacgattca gccagtgtcg ggaaaacttt aatattatca ataatatcac tagatccatg
    19261 tgcgacccag cgggtggctg gctccaggtg tgcctgacta tcgacaatcc gcagttcgct
    19321 aaaacccatc gttttcattg cccgcgccgc tgccccaata ttttctgctc tggcgggtgc
    19381 gaccaggata atcgttatac gcatattgcc actcttcttg accaaataac cgcgaacagg
    19441 gtgatcactg tcaacttatt acgcggtgcg aatttacaaa ttcttaacgt aagccgcaga
    19501 aaaagccctt tacttagctt aaaaaaggct aaactatttt ctgactgcac taacggttta
    19561 attgttaaaa aatgctacat atccttctgt ttacttagga taattttata aaaaataaat
    19621 ctcgacaatt ggattcacca cgtttattag ttgtatgatg caactagttg gattattaaa
    19681 ataatgtgac gaaagctagc atttagatac gatgatttca tcaaactgtt aacgtgctac
    19741 aattgaactt gatatatgtc aacgaagcgt agttttattg ggtgtccggc ccctcttagc
    19801 ctgttatgtt gctgttaaaa tggttaggat gacagccgtt tttgacactg tcgggtcctg
    19861 agggaaagta cccacgacca agctaatgat gttgttgacg ttgatggaaa gtgcatcaag
    19921 aacgcaatta cgtactttag tcatgttacg ccgatcatgt taatttgcag catgcatcag
    19981 gcaggtcagg gacttttgta cttcctgttt cgatttagtt ggcaatttag gtagcaaaca
    20041 tgcagacccc gcacattctt atcgttgaag acgagttggt aacacgcaac acgttgaaaa
    20101 gtattttcga agcggaaggc tatgatgttt tcgaagcgac agatggcgcg gaaatgcatc
    20161 agatcctctc tgaatatgac atcaacctgg tgatcatgga tatcaatctg ccgggtaaga
    20221 acggtcttct gttagcgcgt gaactgcgcg agcaggcgaa tgttgcgttg atgttcctga
    20281 ctggccgtga caacgaagtc gataaaattc tcggcctcga aatcggtgca gatgactaca
    20341 tcaccaaacc gttcaacccg cgtgaactga cgattcgtgc acgcaacctg ctgtcccgta
    20401 ccatgaatct gggtactgtc agcgaagaac gtcgtagcgt tgaaagctac aagttcaatg
    20461 gttgggaact ggacatcaac agccgttcgt tgatcggccc tgatggcgag cagtacaagc
    20521 tgccgcgcag cgagttccgc gccatgcttc acttctgtga aaacccaggc aaaattcagt
    20581 ctcgtgctga actgctgaag aaaatgaccg gccgtgagct gaaaccgcac gaccgtactg
    20641 tagacgtgac gatccgccgt attcgtaaac atttcgaatc tacgccggat acgccggaaa
    20701 tcatcgccac cattcacggt gaaggttatc gcttctgcgg tgatctggaa gattaatcgg
    20761 ctttaccacc gtcaaaaaaa cggcgctttt tagcgccgtt tttatttttc aaccttattt
    20821 ccagatacgt aattcatcgt ccgttgtaac ttctttactg gctttcattt tcggcagtga
    20881 aaacgcatac cagtcgatat tacgagtcac aaacatcatg ccggccagcg ccaccaccag
    20941 cacactggtt cccaacaaca gcgcgctatc ggcagagttg agcagtcccc acatcactcc
    21001 atccagcaac aacagcgcga gggtaaacaa catgctattg cgccagcctt tcaataccgc
    21061 ctgcaaataa ataccgttca ttaacgcccc aatcagactg gcgattatcc atgccacggt
    21121 aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa aacatcacca atgaaagccc
    21181 caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg agcgtttcaa aaacaaagaa
    21241 cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta gtcgcccggt ccgttaattg
    21301 gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg gggaagtttt cccagccggt
    21361 atcattgcct gaagcaaaac gctcaccgag attattagca aaccagctgc tttgccattg
    21421 cgcctgaaaa cctgactcgc tagcttcccg tttggctggt agaaaatcac ctaaaaaact
    21481 gggatgcggc cagttgctgg ttaaggtcat ttcgctatta cgcccggcag gcaccacaga
    21541 aagatcgccg gtaccgctta aattcagtgc catattcagc tgcaggttct gcttccgcca
    21601 gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct tgctctaacc cggtaccggg
    21661 ttcaatggtc agtgccgttc cgttaacttc aggcgctttc accacaccaa taccacgcgc
    21721 atccccgacg ctaatcacaa taaagggctt gcctaagatg atatttggcg cgttgagctc
    21781 gctaagacgc gaaacatcga aatcggcttt tagcgttaaa tcactgtgcc agacctgacc
    21841 ggtataaatc cctatcttgc gttcttccac gttctgattg ccatcaacca tcaatgactc
    21901 aggtaaccaa aaatggataa aacttcgttt ccgctccacg gttttatcct cttcctgcac
    21961 cgtataaagc tcggtcacag gaatagcgat aagcggccca acgagttttt gcggcccgct
    22021 ggtactttgg cgaatcgcat cttccacatc gctacggtaa tcggcacgtt cgacaatcac
    22081 ttgccgaatc agcattatcg gaatcaacaa cagcaatact gcaccaaaca aggtggtcat
    22141 tttccagaac aggggggatt tcaacatagt cttctccttt gcaatggcag caggatacga
    22201 aggctatgtg ggaagaattt gaagctatgt gaagttacgg tgaagtcgta gcgaggccag
    22261 cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg ttaaacaaac gggcgacctc
    22321 actgacgaac gccaacccca gaccgctgct tttttgccca tttgcacgcg gcaaagagta
    22381 aaagcgttca aaaatacgtg acagcgcgta gtcaggaata ccactaccgg tatccagcac
    22441 cttaagcgtg acgtgttcct gatccacttc ggcgcttagc gttatgcaac cgctctcggg
    22501 agtaaaatcg atggtgttat ccagtaaatt ccccagcgcc tgatccagta acgccggttc
    22561 agcagcaaca ttaacctcgg tgggcataac atgcaaagtg atgttttttt ctgccaactg
    22621 cacggtgcgc gcttcgctga cgcggcgaaa taatgccgcc acatcaacag cagtcagaac
    22681 gacttcctga cgattctcca gtcttgcctg gcgtagtaac gtttccacca gcgcctgcat
    22741 tcgcgcattt tgtgtcagaa tgttgtcagt aaaacgagcc actacttcag gcggcggacc
    22801 ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt gggcttttta gctcatgggt
    22861 taacgcataa acatactgct caatatagtt tttcccttcc agcttcacgc gcatactttc
    22921 cagcgcctgc gcgagtttac gcagctcgct actaccgaga tcgggaagag gaacgggctt
    22981 attgtcagtg acggaatcag catagcgagt gagccgggca atggagcggt tgatccacca
    23041 aaccatgcct gcgccaatca ccagtgcaat ccccaacaaa atggcgctgg cccataaaat
    23101 tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg ttcggtttgc ctacgctcaa
    23161 aacgccaata agccgcgagc cgcccataat cggcgcggca acatacatca ccgaactttc
    23221 gggatcggca ggattttgca acgtgctgcg cgcaccatac tgaccacgca acgttagcca
    23281 gacgtcattc cagcgcgaat aatcctgtcc aacggcttta tttgccgaat cgaacaatac
    23341 tttgccatgc gcatcggtca tatagacatg atattcattg cgcactttgt taatgccacc
    23401 aatattggcg cgaaacgggc gatgttgtag ctgattaaac gcctgcgcca gttgcccatg
    23461 cgttgggtcc ccagagagca aatcgggacg cgccagctcc gcaagtaacg ttgccgtgtc
    23521 gatcaacgtc ccctccgttg ctcttcgcac gcccggttta acttctttga caaaaatagc
    23581 cagtacgaac caggccgcca ccgccaccag taaaaaatag cccagcagca accgcatgcc
    23641 gatacgcatt acaagcccct caggctatat cccatgccgc gatgagtatt aatcggtgaa
    23701 agatcagggt tgatggcgcg cagcttggca cgcagcgttt taatgtggat atcgacggtg
    23761 cgatcgtagg tgtcctgcgc atcttcccac acgctatcca ttagttgctg gcgggaccag
    23821 acgcggcctg gcgacttgag taacgtcttc agcaataaaa actcataccg cgtcagcgtt
    23881 aatggcgtgt caaaccagct gatctgcgcc gcgggttcat tcaattcaaa atgaccaata
    23941 cggatgacgg gagacggcgt cgagaacttc ttcacccgac gcagtaaggt gcgcaccctg
    24001 gcgcacactt cgcggggtga aaacggttta gccacatagt cgtcaacgcc aatttccagc
    24061 ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga acagtacagg taacgccgga
    24121 tggagcgcca gtaactggcg gcacaattca aagccactga tatcaggcaa accaacgtcg
    24181 agaatcatga cgtcgggaac ctgctggcga gctttatcca gcaccggcaa acctcgctca
    24241 aagacctcga cggcaaaacc ttcctgctgc aacatgtaga ccagcgtgtc ggctatccct
    24301 tgctcatctt ccactaacca gaccgtttcc cgttgcataa atcgcctctg ttattgccgc
    24361 cacggcatga caggaaccgc gctaatcgcg tttttcggcg aaccttctac aactttgtcg
    24421 gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat aaaagcgcac gacctgtaac
    24481 gacttaaaga ccagggacgt gcgttttttg aatactacct cgccctgagc tttgccgttt
    24541 ttaatacgat ccgacagttc aatcggcccg acttgctgac aagaaatggc cgcatcggag
    24601 gtatcttctg ccaggcccaa tcccccttta ataccaccgg ttttcgcccg gctcacataa
    24661 caggtgacat ttttcacatc gggatcgtca aaggcttcca caacaatttt gtgatccggg
    24721 ccgatcattt taaatacggt gtcgaccgaa ccaatctctt ctgcatgagc caatggcccc
    24781 agcattatca ggcttaaaga caggatcaaa tgcttgtatt tcatattgtt accattttta
    24841 tctattacac gagcaattag ttcgtcacgg taaataacat attgattaag aacaaaatct
    24901 caattctttt atttccgtca ggccctaaaa catactctac taagaaaaaa acactgaatg
    24961 ctaaaacagc aaaaaatgct attatccaat tacctgatgt caggtgctcg ttgttgaaag
    25021 gatgaggata ttttatggat caggccggca ttattcgcga ccttttaatc tggctggaag
    25081 gtcatctgga tcagcccctg tcgctcgaca atgtagcggc gaaagcaggt tattccaagt
    25141 ggcacttaca gagaatgttt aaagatgtca ctggccatgc tattggcgcg tatattcgtg
    25201 ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac tgcgcgtccg attctagaca
    25261 tcgcgctgca ataccgcttc gactctcaac agacatttac ccgcgcattc aagaagcagt
    25321 ttgcccagac tcctgcactt taccgccgtt ctcctgaatg gagcgccttt ggtattcgcc
    25381 cgccgctacg tctgggtaaa ttcacaatgc cagagcacaa atttgtcacc ctggaagata
    25441 cgccgctgat tggtgttacc cagagctact cctgttcgct ggagcaaatc tctgatttcc
    25501 gccatgaaat gcgttatcag ttctggcacg attttctcgg caacgcgccg accattccgc
    25561 cggtgctcta cggcctgaat gaaacgcgtc cgagtcagga taaagacgac gaacaagagg
    25621 tattctatac caccgcgtta gcccaggatc aggcagatgg ctatgtactg acggggcatc
    25681 cggtgatgct gcagggcggc gaatatgtga tgtttaccta tgaaggtctg ggaaccggcg
    25741 tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc aatgctcaat ctgacgcgcc
    25801 gtaaaggtca ggatattgag cgatactacc cggcagaaga cgccaaagcg ggagatcgcc
    25861 caattaatct gcgctgtgaa ctgctgattc ctatccgccg ttaacgctgc agctcatcta
    25921 atgcaggggc gtccagatgc gagatgtccc ctgcggtttc aacgacccag ccagacgcca
    25981 gccacaggct ttcctgataa tccacgcgcg aaatagaaca gttacgcaga cgtaagcggc
    26041 gctctgccca tgctggtaat ccgagaatcg tactcaccag acatcccagt gcaataccat
    26101 ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg gcaagattcc agtgccgcat
    26161 tgacgcgatc gctgagctcc tgcattgact cgccttcagg aatacgcccg tcaacggtgc
    26221 cattgaccag ctgccgacgc cagttctctt cttcttcggt cagagaatcg atatgtcttt
    26281 tttccagcac acccatgttt aattcacgca ggcgagaatc aaagatgatg tcacagccgc
    26341 aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc taaatcgcta ctgataatat
    26401 gcgtaatgcc aagctctttg gcacgggttg ccacctgcat cgcctgttgc tcacctttgg
    26461 cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc ggcgttccac tgcgtttcac
    26521 cgtggcggac taggtatacc tgtaacatgc tttttttccg tatactgctg taacgttaat
    26581 tttctgagta atgctgatta tgcaccaagt tgtctgtgcg accaccaatc ccgctaaaat
    26641 tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa ggatcctgcc atattgcatc
    26701 cgttgccgtc gagagcggtg taccggaaca accctttggc agtgaggaaa cgcgcgctgg
    26761 cgcacgaaat cgggtagcaa atgcccgccg tttacttcca gaggctgatt tttgggtggc
    26821 gattgaagct ggcattgatg gcgacagcac tttcagttgg gtggtgattg aaaacgccag
    26881 ccagcgcggc gaggcgcgtt cggcaacttt gccgttacct gcggtgattc tggaaaaagt
    26941 tcgtgaaggc gaggcgcttg gtcccgtgat gtcgcgttat accggtattg atgagattgg
    27001 tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa ctcactcgcg ccagcgtcta
    27061 tcatcaggcg gtgatcctgg cgctgagtcc gtttcacaat gccgtgtatc aggcattgta
    27121 ggacggataa ggcgttcacg ccgcatccga cattcggtgc acgatgcctg atgcgacgct
    27181 gccgcgtctt atcaggccta caaaatcaat cgcttttcag caacacctct tccagccact
    27241 gacgcagctc cacgggcgcg gctttcaggc tgttagatcc acgcgtaatc gtcgcgatgc
    27301 ccgcgccgag ttcatttttt aactcacgct gactcatttc gccacgcaac agctcttcga
    27361 caatacgcac gcgagtcccc aacgcttcgc gctcatctgg cgtcagcatc aggtttaaca
    27421 acggtaaatg gagatcgttt tggtaggcat tcttaagcag gtcgacaaaa cgtaaccact
    27481 cctggtgacg ctgttctgcc atcgctgctg aatagggtga ttgttgggcc ataatatgtc
    27541 gccattgtta gcgggggaag caaaatgact cccccggttg tactcgctaa agagtacgat
    27601 agcatatcat aaacgtgcgg atcagtaacg acgtccccat tccgtggcgc tcatcaacgt
    27661 cggtttatcc cccatgaaat agcggtagta agcatcataa gccagcacgt tcttcacata
    27721 accgcgcgtc tcggagaacg gaatactctc gacaaatgcc acagcgtcga tacgcccggc
    27781 gctgttgcca agccaagttc gcacccgtcc aggtccggcg ttataagctg ctgaggagaa
    27841 aatacggtta ttgccaaact gctgataaac atattgcagg taactggtgc caatgttgat
    27901 gttcgtttcc ggatccagca attgcccagg gctgctataa ccaggaatag agaacatctt
    27961 caccgtatgg gtcgctgtac caagcataat ctgcatcagg ccgctggccc ctaccggtga
    28021 tttcactttc ggattccagg cgctctcctg acgggcaatc gccatcgcat agctttgcgg
    28081 gatctctttc ccactggtgt aacgtttgaa aagatcgttg taagccagcg ggaatcgctc
    28141 ttccagatga tcccaaagct tcccggcgat cgttgcctga acgctaagat cccaccattg
    28201 gttattgaaa gcataccgag ccagttgagc ctgctctgtt tttgacttgc tcttcaccag
    28261 attggcccac tcgctacgcg cggtattatc gagattccag tacatcaact cgcgcacgcg
    28321 cgccatctcc ggcccctgag tcagggtgct gtcaacattc tgcggcgctt tatcaatctt
    28381 cagctcatac tcttcaccga tgcgttgtgc tgcaaccatc gggtagaaac cacgctgttg
    28441 catgagttga tgcaaaatct ctttcgcttc agcttcacgt ccgcgttcca gcaataaatc
    28501 cgcctgccag taacgccatt catctttctc ttttgcttcc atcggcagac gcgccagcca
    28561 ggtattcagg ccgcgacgat cgccggtgcc aagcgccatt cgcacacggc gttcaatcaa
    28621 cgacgtggat tgcgagcgca taatggcgtc atcgcgccat ttcgcctgct catcggtgac
    28681 atcattgccc atcaaacgcc aggcgacgat atcgcgcagc tcctgaatct gatcttcatt
    28741 aagctgctgc gcctgggcaa gcgacgggat catcagccgt gcattttcgg catcctgccg
    28801 cgccacactg gcaaacgcca ccgccgccat ttgatgggta aaatcggtcg cgccggttgt
    28861 acgcgcgaag gtcagtaccg tattagggtt gttcgccagt gaaatgattg ccgaggcgat
    28921 agtctggtaa tcggcaggca tctgccctgc cagcactgtt accaggcctg tgttacccgc
    28981 tttcatcgcc agacggatac gctctaaata cgccagcgga tcttgtttac ctgacgcacg
    29041 ccagacgcta aataacttgt cacaggcgtt aggctggctc ttgccggtta gccacagctc
    29101 tttcgcccct tgccaggctt cttcactctg cccggtgttc catttcgcat agtagtaatt
    29161 acattgcgct tcggtagttc cgggcttttc cgggctaaag gctaacaagc cacgccagtc
    29221 ttcacgccgc gccagttcat tgacgaaacg agattgcagc gtgcgagcgg gaggaagcgt
    29281 ggggttagcg cgaacaaagt tagtgaccgt caccgccggt tgattcatca ggtcatcggt
    29341 gatctggcgg tattccaggt agggataaag cggataatcc ttcagtccag gcatcatttg
    29401 ttcgaccaca tccatctgtc gattatccca ggcctgcttg atttgcgcgt aacggctacg
    29461 ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc gtcagcagac agacaccggc
    29521 agccaacagc cgccaggtaa cttgtttggc tttttccaca agcgcatcct ctaagtgtaa
    29581 atacatcaat gcagcgtcat accgcgtaat agatttatgc taaccagtca ttgccgttta
    29641 cgccacgtta cggacacttt tttactttta ctgcgaaggt gatcgaccaa ctgggattag
    29701 gcggggaaac aggctacact tcgcctttga aaactcatca tcacgataaa catagaggcg
    29761 aagtccaacg tggctcaatt cgtttatacc atgcatcgtg tcggcaaagt tgttccgccg
    29821 aaacgtcata ttttgaaaaa catctctctg agtttcttcc ctggggcaaa aattggtgtc
    29881 ctgggtctga acggcgcggg taagtccacc ctgctgcgca ttatggcggg cattgataaa
    29941 gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga ttggttatct gccgcaggaa
    30001 ccgcagctga acccggaaca caccgtgcgt gagtccattg aagaagcggt ttcagaagtg
    30061 gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt acgccgatcc ggatgccgat
    30121 tttgacaagc tggccgctga acaaggccgt ctggaagaga tcattcaggc tcacgacggt
    30181 cataacctga acgtacagct ggagcgtgcg gcggatgcgc tgcgtctgcc agattgggac
    30241 gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg tggcgttgtg ccgcctgctg
    30301 ctggaaaaac cagacatgct gctgctcgac gaaccgacca accacctgga tgccgaatcc
    30361 gtggcgtggc tggaacgctt cctgcacgac ttcgaaggca ccgtggtggc gattactcac
    30421 gaccgttact tcctcgataa cgttgcaggc tggatcctcg aactggaccg cggtgaaggt
    30481 attccgtggg aaggtaacta ctcctcctgg ctggatcaga aagatcagcg tctggcgcag
    30541 gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga aagagctgga gtgggtacgt
    30601 cagggaacta aaggcagcca gtcgaaaggt aaagcacgtc tggcacgctt tgaagagctg
    30661 aacagcaccg aataccagaa acgtaacgaa accaacgaac tgtttattcc acctggaccg
    30721 cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta aatcctacgg cgatcgcctg
    30781 ctgattgatg acctgagctt ctcgatcccg aaaggggcga tcgtcgggat catcggtccg
    30841 aacggcgcgg gtaaatcgac cctgttccgt atgatctccg gtcaggaaca gccggacagc
    30901 ggcaccatca ctttgggtga aacggtgaaa ctggcatcgg ttgatcagtt ccgtgactca
    30961 atggataaca gcaaaaccgt ttgggaagaa gtttccggcg ggctggatat tatgaagatc
    31021 ggcaacaccg agatgccaag ccgcgcctac gttggccgct ttaactttaa aggggttgat
    31081 cagggtaaac gtgttggcga actttccggt ggtgagcgcg gtcgtctgca tctggcgaag
    31141 ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac caaccaacga cctggatatc
    31201 gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc cgggctgtgc gatggttatc
    31261 tcgcacgacc gttggttcct cgaccgtatc gccacgcaca tcctggacta ccaggatgaa
    31321 ggtaaagttg agttcttcga aggtaacttt actgagtacg aagagtacaa aaaacgcacg
    31381 ctgggcgcag acgcgctgga gccgaagcgt atcaagtaca agcgtattgc gaagtaatgc
    31441 gtaaaatgcc ggatgcggcg cgaacgcctt atccggccta caaaaccagc aatttcaata
    31501 cgttgtaaaa ctgtaggcct gataagacgc ggtaagcgtc gcatcaggca ttgagcaccg
    31561 aatgccggat gcggcgtgaa caccttttcc ggtctacaac agccttagca tcctcaattt
    31621 cgtggcgggt cacccgtgca ggttttttca aaccttgttt aatctcgaca gcctcttcca
    31681 gagaggtttt caactcatca aaaaaactca ttttatacct cgcctttgag cagtttcgtc
    31741 acggttatcc ttgctccccc atcatctccc gcaccagctc cacgcagcgc aggaaacggc
    31801 tgtcgtaatc gtcctcttca acccgcacga attcgatatt gttctcttcc agcatctcca
    31861 ccagcaagtt ctggaactct ttgcgatcca ccgaactgcc gaggctgcgt aaaccatccg
    31921 ccacccacgg cgtgttgttc tccagcagga tcaccagatc gaaacggtat tcatcaatca
    31981 gcgcctgcac gaacggatgc tcacgccctt cgtacttttt gcagaacgcc tgggtagtga
    32041 caaaatcggt gtcaataaac gccactttat tggcgtattt cactgcaaaa tcaatgtatt
    32101 gtgcgtggcc caacgcgatt ttatcgtagt cggaatactg caatgcgatc tcatcaccgc
    32161 cgaggtgtga aaagacataa tcgcggccat attcccacgc actggtggtg ttgaagatat
    32221 tggcaagttt gtttaccagg gtggatttac cgctcgactc gccgccaagg atcgccacgg
    32281 tacgcacaaa aaacggcttc acttcggtag gaatatattc ccagtagcgg aacgggtttt
    32341 cgcggatctg cgcaccgctg atactcataa aggtacgttt cggatcaacc agcaccgtct
    32401 cgatccccag atgttccata tactgtggcg catcggcttc ttccgaggtg tagatcaggt
    32461 ccggctggat ccccttttca gccataaact ttttgatgcc gttgctccac acatcccagc
    32521 catgcggata cggctccatg ccctcttcgt tgaaagcatg aatacggata tttttctgat
    32581 atttaaaggt ttgcaatagc cagcgcagac gatccggcac cgtgggctgc tgcgacatgg
    32641 cgctgtcttc aaacaacgcg cggtcgcggg tatcgtcaaa acccataatg atatgcagct
    32701 cgtcaacctg gctacaggcg cgctggataa ggtagatatg tccggtatgc agcgggtaga
    32761 acttaccgaa tacgacacca atggttttct tctgccgggg aaattcaagc cccaaaaaac
    32821 ggtgcaatgc ctccagcttt tgcgcgctgg ggcttttaat tttggcattc agtaactggc
    32881 ttaaataccc tttggtcata ccgctggcat cagctacctg ctgtagcgtg cagccctgtt
    32941 gcttgatggc agttttcagg taatcaaatg acgacatatc tccctccgta tctctcatta
    33001 taagtcgtcg aacacgctaa gcgcgtcgga gagtttttta acgccaaaaa tctgcatccc
    33061 ttccggcgct tttttcggta cgttagccgc cggaacaatc gcccggcgaa aaccgtgttt
    33121 cgccgcttca gaaatccgct cctggccact gggcaccggg cggatctccc ctgccagccc
    33181 gacttcacca aacaccacca gatcctgtgg cagcggtctg tcacgcaggc tggaaaccat
    33241 cgccagcagt aacgctaagt cggcgctggt ttcggttacc ttcacgccgc cgaccacgtt
    33301 cacaaacaca tcctgatcgg ccatttgcag accaccgtga cggtgcaaga cagccagcag
    33361 gattgccaga cggttttgtt ccagccccac tgccacgcgg cgcggattcg ccattatcga
    33421 gtgatcgacc agcgcctgaa tctccaccag caacggacgc gttccttccc acaccaccat
    33481 cacggagctg ccggaagtca cttcatctcc gcgacttaag aaaattgccg aagggttgct
    33541 gacttcacgc agcccctgtt cggtcatcgc gaagacgccc agctcattca ccgcgccgaa
    33601 gcggttttta tggctgcgta aggtgcgaaa acgggagtcg gcatcgccat ccagaagcac
    33661 cgaacagtcg atacagtgtt ccagcacttt cgggccagcc agcgaaccat ctttggttac
    33721 gtgtccaacc atgacaatcg ccacaccgcg cgttttggcg aaacgcgtca gataagccgc
    33781 cgtttcacgc acctgcgcca cgctaccagg cgatgattgt acatccgcca tatgcatcac
    33841 ctggatcgag tcaattacca tcagcttcgg ttgctcttct tcggcaatca ggcagatctg
    33901 ctcgatgctg gtttccgaca acatattgag attgtcagtc ggcaggccaa ggcgatgagc
    33961 gcgcattgcc acctgttgca gcgactcttc gccggtgaca tacagcgttt tcatctgctg
    34021 ggccagtttg cacagcgttt gcagcaacag cgtggatttc cccgcaccag ggttaccgcc
    34081 aatcagaatg gcacttcctg gcaccacgcc gccgcctagt acgcggtcga actctttaaa
    34141 tccggtggaa aaacgcggca gctcttcaag gctgatatcg gagagtttct ggacttttgc
    34201 caccccggcg ctaccggcat agccgctgag acgctcgtta cgcgccaccg ttggcgacgc
    34261 agcaagacgc acctcggtga tggtgttcca ggcatgacag gcactgcact gcccctgcca
    34321 gcgcggataa tcggccccgc attcattaca aacaaaggcg cgttttggag cttttgccac
    34381 ggtgtacctc gttaatgctg tgccgcccgc aggatgacgg gcaggcaatt acttctgatt
    34441 caggctgcca gagaggatgc agaatacccc catcaggtca gcgtgacgga tggtgacttc
    34501 cgccttttca ttcacttttg gcttggcatg gtaggcaatc cccagccctg ctgctttgat
    34561 catcggcagg tcattagctc catcgccaat cgccacggtc tgcgccagcg ggatttcata
    34621 ctcctgcgcg aggcgagtca gagttttcgc tttgtactgt gcgtctacga tgtcgccgat
    34681 cacattgccg gtaaatttac cgtccatgat ctccagttca ttggctagca cggctgtcag
    34741 gcgcagcttg tcgcgcaggt attcagcaaa gaaagtaaag ccgccggagg caatcgccac
    34801 tttccagccc agcgtttcca gcttgagcac cagctgcgtt aagcctggca tcagcggcag
    34861 attttcacgc acctgttgca gaatattggc gtcagcgcct ttcagcgtcg ccacgcggct
    34921 gcgcaggctg gcggtaaaat cgagttcgcc gcgcatcgcc cgttcggtta cctccgctac
    34981 catctcgccc gttccggcca gcttggcaat ttcatcaata cattcaatct ggatggcggt
    35041 ggaatccatg tccatcacca gcaaacccgg cgtgcgcagg tgcgggattt tccccagcgg
    35101 ggcgacatcc agctgcgctt cgtgggccag gcgtgtagcc cgtgcggtga gtgaacctgc
    35161 cagacgaatc acctgataat cttccacgca ccaggcggca acaatcacca tcgccgcacc
    35221 cagtttgctc tggtattggg tcagacgctg tttatccagc ccacgaccat acagcagcca
    35281 gccgctacga gctgcgtggt aatccagtgg catcacttca tcaccactta atgaaagagg
    35341 cagacccggc cataaagaga catcttcagg caggtcgcac caggtaatgt taggcattaa
    35401 ggctcctgta aaatcgttcg aagcagggaa aataacgcat gaggctacct tgtatccatt
    35461 gcttctggca acattaagtc tcaaattttc aaagggtgga agatggctcg cacaaaactg
    35521 aaattccggc tacatcgggc agtgattgtc ctgttctgtc ttgccttgtt agtggcactg
    35581 atgcagggag cgtcatggtt tagtcaaaac caccagcgac agcgtaatcc acagctggaa
    35641 gaactggccc gcaccctggc gcgtcaggtg acgctgaacg ttgcaccgct gatgcgtacc
    35701 gactcaccgg atgaaaaacg cattcaggcg atcctcgatc agttaacgga tgaaagccgt
    35761 atcctcgacg cgggtgtgta tgacgaacaa ggcgatctta tcgcacgttc tggcgaaagc
    35821 gtcgaagtgc gcgatcggct ggcgctcgac ggtaaaaaag caggcggcta ttttaaccag
    35881 cagattgtcg agccaattgc gggtaaaaac ggaccgctcg gctatctgcg cctgacactc
    35941 gacacccata cgctcgccac cgaagcccaa caggtggata acaccactaa cattttacgc
    36001 ctgatgttgc tgctctcact ggcaatcggc gtagtgctga cccgcacgca gctacagggt
    36061 aaacgcaccc gctggcagca atcgcccttc ctgttaaccg ccagcaaacc ggtgccggaa
    36121 gaggaagaaa gcgagaaaaa agagtgaccc attactacaa gaaaggaaat cgttatgtcc
    36181 acattacgcc tgctcatctc tgactcttac gacccgtggt ttaacctggc ggtggaagag
    36241 tgtatttttc gccaaatgcc cgccacgcag cgcgttctgt ttctctggcg caatgccgac
    36301 acggtagtaa ttggtcgcgc gcagaacccg tggaaagagt gtaatacctg gcggatggaa
    36361 gaagataacg tccgcctggc gcggcgcagt agcggtggcg gcgcggtgtt ccacgatctc
    36421 ggcaatacct gctttacctt tatggctggc aagccggagt acgataaaac tatctccacg
    36481 tcgattgtgc tcaatgcgct gaacgcgctc ggcgtcagcg ccgaagcgtc cggacgtaac
    36541 gatctggtgg tgaaaaccgt cgaaggcgac cgcaaagtct caggctcggc ctatcgcgaa
    36601 accaaagatc gcggcttcca ccacggcacc ttgctactca atgccgacct cagccgcctg
    36661 gcaaactatc tcaatccgga taaaaagaaa ctggcggcga aaggcattac ttcagtgcgt
    36721 tcccgcgtga ccaacctcac cgagctgctg ccggggctca cccatgagca ggtttgcgag
    36781 gccataaccg aggccttttt cgcccattat ggcgagcacg tggaagcgga aatcatctcc
    36841 ccggacaaaa cgccagactt gccaaacttc gccgaaacct ttgcccgtca gagtagctga
    36901 gagtggaact tcggtcaggc tccggcattc tcgcatctgc tggatgaacg ctttagctgg
    36961 ggcggcgtgg aactgcattt cgacgttgaa aaaggccata tcacccgcgc ccaggtattt
    37021 accgacagcc tcaaccccgc gccgctggaa gccctcgccg ggcgactgca aggctgcctg
    37081 taccgcgcgg atatgctgca acaagagtgc gaagcgctgt tggttgactt cccggaccag
    37141 gaaaaagagc tacgggagtt gtcgacgtgg atagcggggg cggtaaggta actcgctgat
    37201 taaaatatcc aaggaaggaa atatgatcga tctggaaaac caggaacggg agattatcaa
    37261 tttaatgttc agccagcgta tttcctggct agccgccgtg cgtatcaggc ataaattatc
    37321 gcttgctgaa gtaagtaaaa tgttaggtat ctcgattaat tctctaaaac agatcgaaaa
    37381 gacggaacgg cttagctcaa acatcaaaag caaaatggca gaaatttatg gttgtccacc
    37441 agagttgctg atatgccctt cctggatgac ggctgaacat aaatgaatag attcgtgaat
    37501 agatacttga atgtatttta ttttctatcc atatgaaaaa taatagtaaa tctataaatc
    37561 agttgctatt gatgaataga tactaaaaag ccggagcagt cccccactcc ggcttaacac
    37621 gacaatcgcc tttcagcgaa acacaattac tctttatcgc ccagcagaac ggattccagt
    37681 gcgattttga tcatgtcgtt gaaggtggtc tgacgctcag cggcagtggt ctgctcgtga
    37741 gtgcggatgt ggtcagacac ggtgcagatg gtcagggctt tcgcgccaaa ctctgcagcc
    37801 acgccgtaga taccagccgc ttccatttcc acgccgagga tgccgtattt ttccatcacg
    37861 tcgaacattt cgccgtccgg agagtagaac aggtcagcgg agaacaggtt acccacgcga
    37921 gcatcaacgc ccagtgcttt agctgcatct actgcgttac gcaccatgtc aaagtcagcg
    37981 atagcggcaa agtcatggtc tttaaaacgg atgcggttaa ctttggaatc ggtgcaggcc
    38041 cccataccga taacgacgtc gcgcagtttt acgtgcggca gaactgcgcc acaggaacca
    38101 acgcggataa ttttcttcac gccgaaatcg gtgatcagtt ctttggtgta gatggagcag
    38161 gacgggatac ccataccgtg acccattacg gaaattttgc ggcctttgta agtaccagtg
    38221 aagcccagca taccgcgaac gttgttcact tcacgggcat cttcaaggaa agtttcagca
    38281 atatacttcg cacgcagcgg gtcgcctggc atcaaaacta cgtcagcgaa atcgcccatt
    38341 tctgcattaa tgtgtggggt agccattgtt ttatcctttt tgtgacataa caaaggcgga
    38401 gtcaacgctc cgcccaaatc catcagaaca tggctttgcc atattccata tcagaagtac
    38461 caaaatattt tgccagagtc tggccgatat ccgcgaaggt ttcacggtga cccagtgagc
    38521 ccggttttac tttcgggcca tataccagta ccggaatgtg ttcacgcgtg tggtcagtac
    38581 cggtccaggt cggatcgcaa ccgtggtcag cggtgaggat caggatgtcg tcatcgcgca
    38641 gcagagacat cagctccggc agacggcggt cgaacagttc cagacccgca gcataaccgg
    38701 cgacgtcgcg acggtggccc caggaagagt cgaagtcaac gaagttggtg aagacgatgg
    38761 tgttatcacc cgcttctttc atctctttga tggtggcgtc aaacagcgcg tccaagccag
    38821 tcgctttcac ttttttggtg ataccgcagt tggcgtagat gtctgcaatt ttaccgacag
    38881 aaaccacctg gccgtgtttt tcatcaacca gtttctgcag cacggtcggt gctggcggct
    38941 caacagccag gtcgtgacgg ttaccggtac gctggaagtt accagctttg tcaccgataa
    39001 acggacgagc gataacacga ccgatattgt agccgccgtt ggtcagctct tcacgggcga
    39061 tttcgcacag ttcgtagagt ttatccagac cgaaagtttc ttcatggcag gcaatctgga
    39121 acacggagtc agcggaggta tagaaaatcg gcttgccggt tttcatgtgc tcttcgccca
    39181 gttgatccag aatgaccgta ccggaagagt ggcagttacc gaggtaaccc ggcagattag
    39241 cgcgttcgac cagtttatcc agcagctctt gcgggaagct gttttcgtga tcggagaaat
    39301 atccccactc aaacagaacc gggacaccgg cgatttccca gtgaccagac ggggtatctt
    39361 taccggatga catttcgtgc gcccatgcgt acgcgccgat aacttcagcg ttgccgtcca
    39421 ttcccgccgg aatgaaaccg gtagaacctt cgtgtgcttt cgccagcccc agacggatca
    39481 gatttggcag attgagcggc cctttacgac cattatcagc ttcgcctttg gcacaagctt
    39541 ctgcgatatg acccagggtg tcagccccga cgtcaccaaa gcgttctgca tcttctgtag
    39601 cgccgatgcc gaatgagtcc agcaccataa taaatgcacg tttcatatgt tctccgtact
    39661 cagtgcttca aatttaaatg atcagatcag tataccgtta ttcgctgata cggcgataga
    39721 cagttggtgt gctttccggt gcgttatcgg caagtttaat tgccgctttc accgctttcg
    39781 ccgcttcctg ccagctgttt tcgtctttcg cgaggataac cgccagcgga cgctgaccgt
    39841 ctacctggtc gcccagacgc gccatatcag taaagccgac gctgtaatcg atggtgtcag
    39901 atgcctgacg acgaccgccg cccattgcaa ccactgccat ccccagcgcg cgggtatcca
    39961 tttcactgac aaaaccttcg gtatcagcat agactgcttt cgtcagcatc gctgtcggca
    40021 gatacttcgc gtagttctca acgaagtcgg tcgggccttt ttgtgccgct accatacgac
    40081 caaagacttc tgccgcttta ccgttgtcca gcaccgcctg caatttcgcg cgcgcttcgg
    40141 cgtcatcttt cgccagtttg ccggagatca gcatctccac gcacagcacc atcgtgacat
    40201 caaacagacg cgggttacga tactcacccg tcaggaactg caccgcttca cgaacttcaa
    40261 ccgcgttacc tgcactggag gccagtacct gattcatgtc ggtgagcagc gcggtggtgc
    40321 gcacgccagc gccgttagcc acgccaacaa tcgcttcggc aagggcttca gagagttcgt
    40381 aggtcggcat aaacgcgccg ctacccactt tcacgtccat caccagcgcg tccagacctt
    40441 ccgccagttt cttcgccagg atcgaggcag tgatcagcgg gatggagtcc acggttgcgg
    40501 taatatcacg ggtcgcgtag aaacgtttat cagccggagc cagtgaactg gtctgaccga
    40561 taatcgccac gccgacgtct ttaataattt cgcggaaacg gttgtcatcc gggaaaatgt
    40621 cgaagccagg gatggattcc agtttgtcga gcgtaccgcc agtatgaccg aggccgcgac
    40681 cggagatcat cggaatatag ccgccgcagg ctgcgaccat cggccccaac atcagcgaag
    40741 tgacatcgcc gacgccgccg gtcgagtgtt tatcaacaat cgggccattc agatgcaggc
    40801 ttttccagtc gagaacggtt cctgaatctc gcatcgccat ggtcagcgag acacgctcag
    40861 gcattgtcat atcgtggaag aaaatggtca tcgcgagggc ggcaatctgc ccttcggaaa
    40921 tagtgttgtc gcgaataccg ttgataaaga aacgaatttc ttcatcgctc agcgcatgac
    40981 catcacgttt tttacgaata atttcttgtg cgagaaacaa ggtaacctcc cgaggaaaag
    41041 agtatgaggc ggcttttgcc ggatgcgtct acgccttatc cggcctacgg gtagatacgt
    41101 ctcgtaggcc tgataagcgc agcgcatcag gcgtaaagca tcttacttag tagctgctgg
    41161 cgctcttacc gtcgccgtgg cccagcgctt tcagcaggct tgccagcagg ctggaagcac
    41221 caaagcggta gtgacgcgca tcagcccagt cagcaccgaa cagttcatct gcgatggcga
    41281 gatatttctg cgcatcttcc gcagtacgca cgccgcccgc cggtttgaaa ccaacggttt
    41341 tttctacgcc catataacgg atcacttcca tcatgatgcg agcgctttcc ggcgtcgcgt
    41401 tcacagccac tttaccggta gaggttttga tgaagtccgc acccgctttg atggagattt
    41461 cagacgcttt acggatcagc gcttcgtctt tcagttcgcc ggtttcgatg atcactttca
    41521 gcagtacatt cgctgccgcg caagcctctt tacaggcttt caccaggtca aaaccaacct
    41581 gctcgtcacc cgccatcagc gcgcggtacg ggaacaccac gtcaacttca tcagcaccgt
    41641 aggcgattgc cgcacgggtt tctgccagcg cgatttcgat gtcgtcgtta ccgtgtggga
    41701 agttggttac cgtagcgata cggatttccg gggtgccctg ctctttcaga gttttgcgag
    41761 caatcgggat aaaacgagga tagatacaga tagcggcggt attgccgacc ggagttttgg
    41821 cctgatgaca cagagcaatt actttctcgt cggtgtcgtc gtcattcagg gtggtcaggt
    41881 ccatcaattt cagtgcacgc aggctgcttg ctttcagatc agtcatttca ttctcctggc
    41941 ttgtcgccaa taaaattcac cttgcgagtt tgttagtatt ctaacatcta ctccgcaaca
    42001 cacttcgata cacatcacaa ttaaggaaat ctacttacaa gtttgcatca ctgtaatgcg
    42061 atctggttca aataattcac tttcaaatga atgcgtcagt ggtggcaaac gcatcaggat
    42121 caaagtgaac atcacgaaac ttcttacaat ggcgcatcac cttcggcata aaaggaaaag
    42181 aagatgccca cttctcatga aaatgcactg caacaacgtt gccagcaaat tgtcaccagc
    42241 ccagtgctta gcccggagca gaagcgccat tttctggcac tggaagcaga aaacaatctg
    42301 ccttacccac agcttcctgc cgaagcccgc cgcgcgctgg atgaaggtgt aatctgcgat
    42361 atgtttgaag gtcatgcgcc gtacaaaccg cgctatgtct tacccgatta cgctcgtttt
    42421 ctggcgaacg gttccgaatg gctggagctg gaaggcgcga aagatcttga tgacgcactc
    42481 tctctgctga ccattcttta ccaccacgta ccgtcggtca catcgatgcc ggtctacctg
    42541 gggcaactgg atgcgttgtt gcaaccgtat gttagaattc taacacaaaa cgagatcgat
    42601 gttcgaataa aacgtttctg gcgttacctc gacagaaccc tgccagatgc ctttatgcac
    42661 gccaatatcg gcccgtctga ttcgcccatt acccgcgcga tcttacgtgc agacgcgcag
    42721 gtttcaccga acctgacctt tatctacgat cctgaaatca cccctgatga cctgctgctg
    42781 gaagtggcga agaacatctg tgaatgtagc aaaccgcaca tcgccaacgg tccggtgcat
    42841 gataaaattt tcacaaaagg gggctacggg attgtgagct gttacaactc actgccgctg
    42901 gcgggtggtg gcagcacgct ggtacgcctt aacctgaaag ccattgccga gcgcagcgaa
    42961 tcgctggatg acttctttac gcgcactcta ccgcactact gccagcagca gatcgccatc
    43021 atcgatgcgc ggtgtgaatt cctctatcaa caatcacact tctttgagaa tagcttcctg
    43081 gtgaaagaag ggctgattaa ccctgaacgt tttgtgccaa tgtttggcat gtatgggctg
    43141 gcggaagcgg ttaacttgct gtgtgagaaa gaagggattg ccgcgcgcta cggtaaagaa
    43201 gccgccgcaa atgaagtggg ttatcgcatc agcgcgcaac tggcggagtt tgtcgccaat
    43261 acccccgtga aatatggctg gcaaaaacgc gccatgttac acgcacagtc ggggatcagt
    43321 tccgatatcg gcaccacgcc gggcgcgcgt ttgccgtatg gcgatgagcc agatccgatc
    43381 acccatctgc aaactgtcgc cccgcatcat gcttattatt attccggcat cagcgacatt
    43441 ctgacgctcg acgaaaccat caaacgtaac ccgcaggcac tggtacagct ttgcctcggt
    43501 gcctttaaag ccggaatgcg tgaatttacc gccaatgtca gcggtaacga tctggttcgc
    43561 gttaccggtt atatggtgcg tttgtcggat ttagaaaaat atcgcgccga aggttcacgc
    43621 accaacacca cctggctggg cgaagaagcc gcacgcaaca ctcgtattct ggaacgccag
    43681 ccgcgcgtga taagccatga acagcagatg cgctttagtc agtaagatta tccccttctc
    43741 ctgcgttgac gggccaggca gtcgtctggc tctgtttttg cagggctgca atctgcgctg
    43801 caaaaactgt cacaatccgt ggacgatggg acgttgcaat gactgtgggg agtgcgtgcc
    43861 acagtgtccg catcaggcgt tgcagattgt tgacggcaaa gtggtgtgga acgctgtggt
    43921 ctgcgagcaa tgtgatacct gcctgaagat gtgtccgcaa catgccacac caatggcgca
    43981 atccatgagc gtggacgaag tgcttagcca tgtgcgcaaa gcggtgctgt ttattgaagg
    44041 gataacggta agcggcggcg aagtcacgac ccaactgccg tttgtagtgg cgctgtttac
    44101 tgctatcaaa aacgatccgc aactgcgcca tctcacctgt ctggtggaca gtaacggtat
    44161 gttgagcgaa accggctggg aaaaattact cccggtgtgt gacggcgcaa tgctcgatct
    44221 caaagcatgg ggtagcgaat gtcatcaaca attaaccgga cgcgataatc aacagattaa
    44281 gcgcagcatc tatttgctgg cagagcgcgg caagctggcg gaactgcgtt tgctgatgat
    44341 cccaggccag gtggattatt tgcaacacat cgaagaactg gcggcgttta tcaagggact
    44401 tggagatgtt ccggttcgcc tgaacgcgtt tcatgcccac ggcgtgtatg gcgaggcgca
    44461 aagctgggcg agcgccacgc cggaagacgt tgagccgttg gctgatgcat taaaggtgcg
    44521 cggggtgagc cggttgatat ttccggcgct ctatttgtga catgccggat acggcctacg
    44581 gttcggcaca gacttgtagg cctgataaga cgcgtcaagc gtcgcatcag gcattgtgcg
    44641 ccaactgccg gatgcggcgt gaacgcctta tccggcctac ggttcggcac aaacctgtag
    44701 gcctgataag acgcgttaag cgtcgcatca ggcattatgc gccgactgcc ggatgctgcc
    44761 tacgaagggc aaactaccca cgaacgttaa acaccgcata cgtgttatta agcaacactt
    44821 cggcaatctc atccgcgggt tccgggcgta actcgcaaag cacggcaaac acacgggcag
    44881 cctgttccgg gcggttaggc tgcccctgaa aaccgttgag cggcatatcc ggcgcatcgg
    44941 tttccagcaa caacgatgcc agcggtaatt ttgcgatgac atcgcgggtt ttactggcgc
    45001 gtggataggt gatagtaccg cctacgccga ttttgtagcc cagctgtaca aaccgttcag
    45061 cctgttgctg gctgccagaa aatccgtgga caacaccagc acgcggcaaa tcgtggcgtt
    45121 taagatgcat cgccagtttg tcgtgcgtgc gccgtgaatg caggatcacc ggcagatcgt
    45181 agcgtttcgc cagtttcagt tgttcgtcga gtaaccactg ctgcctctca aattgcggat
    45241 cgtcgccgaa gagatccaga ccgatctccc ccaccgccac caccttcgcc ggacgccttt
    45301 ccagcgcctg ctgtagctgc tcaagagaca catcgctatg tttttccaac ataccaggat
    45361 gcaagcccaa tgcggcatac agcggttgat aattttccgc taacgcctgc acgcgggcaa
    45421 aattttccgc ctctgtcgcc ggaacaatga tcttgcctac gcccgcttgt gccgcgcgtt
    45481 gcaggctggc ctcttcatcg ccaataaaag gcgggaaatc aaaatggcag tgagtgtcga
    45541 taaaacggca aatcaagcca gatcctcatt gttaaatgtc gggtcgttcg cctgcggagc
    45601 gtcgctcact tcggcaacca gcgtatcgtt ggcgactggc gcaggcggaa tgacaatcga
    45661 ttccggcgtc accactggca ccagatggcg ggtaagcggc gctttttcag ttaatagctt
    45721 gccaaccgtg gcgaggaaat aacgaccgca taaacgccca agtttatagt cttcacgcag
    45781 cgccggaatc cgactgccaa gcgcgatact atgtaatggc ttcggcggat aaatttcgga
    45841 tatccgcagc ttgcccggtg gtttctcaat aaattgctga atgtcacgat agctggtttc
    45901 atgatgctgc accagattga ccagcggctg caggctactg ccacccagcc agcgttccat
    45961 gcgtttgaac cactgcggcg tgtagtacat ttgtgacggc acagtgcgga tgacgaccaa
    46021 cgttttagcg ccctgccttg ccgcctcttt aaccggaatc gcatcactga tcccgccatc
    46081 caggtagtta atgccttcca gcgacactcc gctacgataa aagccaggta tcgcactgga
    46141 ggcgcgaatc acatccagcc agttttgttt ggttggtaaa aagtaattcg gcgcgtagtc
    46201 atcctgacga caggcgcaca tataaaacga tttgccgctg tcaaacaacc gcgcggcggt
    46261 atccatttgc aacggcatct ggcttgcggt ggcctccacc agccagtcga gatcgataag
    46321 atttcctcca cggacaaagc gcaatggatc gaaaaattcg cgttttgtgg tatagcgcat
    46381 gatgactttg cgcgcgtaac cgggctgatt gcagataaac gccgagaggt tctgcgcccc
    46441 ggcagatgtg ccgagataaa gatcgaaagg attaaactgc gcgcgcataa actcatccag
    46501 cacgccagcc gtgaaaattc cacgctgtcc gccgccttcg cacaccagag ctattcgtcc
    46561 aggctggaac ggccttagcg acaacggcgc aatattacca agcgttacag gtattcgctg
    46621 ccccacttct gccttcctgt ttttgttgtg aaagaaagac aaagtaacgc aatttattat
    46681 tcgctaaaac cgcaaaagcc agtccgctgg actggctttg gataaaattt tcttaatggt
    46741 ctgaaatcta gggtcgtttt cggcccatga acaaactcac taggaacaga ataatcccga
    46801 cgacaaagac aattttagct gcgcctgcag cggtaccggc cagaccacca aacccaagtg
    46861 cggcggcgat taacgcgata accagaaata tgatgcccca acgaaacata agcgtctcct
    46921 ttaccatagt taatgtcacc gcttgatatg agcgtgaaaa tcactcaatg cgatattttt
    46981 agtgtggtgc acatcgcgcc tcccgacaaa gttcgggagg acgaattacg acgaattact
    47041 tagttttcag atcattttta acgcttttca caccatctac cgctttggcg atactttcag
    47101 cacggtcact ttgtgcctga gaatctacgg taccggagag ctgaaccacg ccatcggtgg
    47161 tttcaacttt cactttacgt gaagggacga tatcgtccgc cagcagtttg gctttgattt
    47221 cactggtggt ggcggtgtca cccgcgtagt ccttcaccga gccttcttta gcgtcgcgaa
    47281 cgtgcagttt gtcgctgaca gaggtcacgc cttcaacgcc tttcgccact ttcactgcct
    47341 cttcggcctg ggcctgactt tcaacgaaac cgctcagggt cacgactttt tgatcggttt
    47401 tgacagagat atcggtgctc ttgatgttgt catgatccac cagcgccgcc ttcactttcg
    47461 cggtgatggc gctgtcatcc atgaaattac cgactttatt catagagcta tcgacttttt
    47521 gccctgcgct ttcattggta gtctgcgcgt tgttttccgc gtaggcagag ccggtcgcga
    47581 cggcagaggt caacattaca gccagcagag ttttcgaaat cttcagtctt gtcatagtca
    47641 tcgatttatt cctgtatgtt tgctcgtaat ttgagcctag caacacgagg ttgcattgct
    47701 gaatagggag agacttcacc ctctacagaa gtcaatggtc gccatcacaa aagcgatggg
    47761 tgatgaataa cgaccattac agtctctgaa tcagttatta atatcggtag aatgacaatc
    47821 gacggcttta gatactgata tctacgcatt gaacggtatt taacgccatc agaaatgtca
    47881 tcactttgtt aaatatagat cacaattttg aaaccgctcg ggatatcagc gaaaacataa
    47941 gcaaaagtga atgttttaag aacattccgt aagcggctaa taaggaaggg aaattgacag
    48001 ggcgcagcgg ctaccgcgcc ctggaggcaa gaattaatgc tcgcgggtct ggtggaactg
    48061 aacgtccgga taacgttcct gtgccaggcg caggttgacc atgctggtag cgatgtaagc
    48121 gaggttatcg ccgccatcaa gcgccagttg gctttcgttc ttacgcttga actcttcgaa
    48181 tttcttcgcg tctgcacatt ctacccagcg ggcagtggca acgttgactg actcatacac
    48241 tgcttcaacg ttgtattcgc tcttcaggcg cgctaccacc acatcaaact gcagcacacc
    48301 aaccgcacca acgatcagat cgttgttgga gattggacgg aacacctgca ccgcgccctc
    48361 ttcggaaagc tgtaccagcc ctttgagcag ctgtttttgc ttcagcggat ctttcaggcg
    48421 gatacgacgg aacagttctg gtgcgaagtt cggaataccg gtgaacttca tcatctcacc
    48481 ctgggtaaag gtgtcgccga tctgaatggt gccgtggttg tgcaggccga ggatatcgcc
    48541 cggatacgct tcttcaacgt gcgaacggtc acccgccata aaggtcagcg cgtcggagat
    48601 caccacatct ttcgcagtgc gcacctggcg cagtttcatg cctttttcat atttaccgga
    48661 caccacacgc ataaacgcca cgcggtcgcg gtgtttcggg tccatgttgg cctgaatttt
    48721 aaatacgaag ccggtaaatt tatcttcgct cgcttctacg gtacgggtat cggtctgacg
    48781 cggcatcggc gcaggcgccc actccaccag gccattcagc atatgatcaa cgccgaagtt
    48841 acccagcgca gtaccgaaga ataccggagt gatttcgccc gcaaggaaca gctctttgtc
    48901 gaactcgtta gacgcgcctt taaccagttc cagttcgtca cgcagctgct gtgccagatc
    48961 ttcaccaacc gcagcatcga gatccgggtt attcagccct ttaacaatgc ggacttcctg
    49021 aatggtgtgc cctttaccgc tctgatagag ataggtttca tctttataaa ggtggtaaac
    49081 gcctttaaac agcttgccgc agccaattgg ccaggtgatc ggtgcgcagc caattttcag
    49141 ctcgttctca acttcatcga gaagttccat cgggtcgcgg atatcacggt caagtttgtt
    49201 cataaaggtg aggatcggcg tgtcgcgcag acgggtaact tccatcagct tacgggtacg
    49261 atcttcaaca ccttttgcgg cgtcgataac catcaggcag cagtccaccg ccgtcagggt
    49321 acgataggta tcttccgaga agtcttcgtg ccccggggtg tcgagcaggt taaccaggca
    49381 atcgtgatac ggaaactgca tcacagacgt agtaatggag atcccacgct gcttttccat
    49441 ctccatccag tccgacttag cgtgctggtt ggaaccacgg ccttttactg taccggcggt
    49501 ctgaatggcc tgtccgaaca gcagcacctt ctcggtgatg gtagttttac cggcgtccgg
    49561 gtgagaaata atggcaaaag tgcggcgctt cgccacctct tgcaaataag gagacaacgt
    49621 cataattctt tcttcttgag taagcggcat cgcgccgcgc ttgttgaaaa cgaaaaattg
    49681 cggctatttt acccatcaac gggggggagg caatcagtgt ttacacagga gctgctccag
    49741 ttcgtgcaac gaagaaacgg tccaggtggg cgcgatgcct tctggttgct cgcgattgtg
    49801 cgcattcagc cagcaggtcg caagcccggc gttgatgcca ccgagaatat cggactcggc
    49861 agtgtcgcca accatcagca cgcgtgaacg gtcaggattg cccgcctgtt ccagcgcata
    49921 atcgaaaatt ttcttattcg gtttggcaac gccaacttct tcggaaatca ccagcaaatc
    49981 gaagtaatca cgcaggcccg tccgttccag acgcacttgc tgcaaggcgc taaaaccgtt
    50041 ggtgatgatg ccgattttag cgttgccacg aatggcgtta agcagagaaa ccgcgcccgg
    50101 caacggcgta cagatttccg ccatcgcatt aataaaggca tcgttaagca agcctgcttc
    50161 aactttcagc cgttcggccc agctctcgaa acgcccgtgc tgaagctgta atgaagtgat
    50221 cgcgccgttt tgataatcca cccacagtgg cttgttaacg gcctgatagt cctgaaaatc
    50281 ttcagcggta aaggtgacgc tgtaatcaag aaacatccgc tgcaggccgg tgaatgagtc
    50341 aaaggtaaac agcgtttcat cggcatcaaa gaaaatccag tcccacttca ttattccacc
    50401 ttgtcttaca tactgattgg caacgccatg atgatggcgt cttcgcgacc gtccgtggtg
    50461 gggtagtaat tgcggcgaat cgtcgcctcg ttaaagccta aactttcgta cagggcaatg
    50521 gcggcagcat ttgaggcacg gacttccagc catagtgtcg ccacgccgcg tttttccagt
    50581 tcgtcgatca gatgttccag cagcgcccta cccagcccct gacgctgata gtcgggatcg
    50641 accgcaatat tgaacaatgt agcttcatcc aacaccactt gcgtaatcgc aaacgccgcc
    50701 attttgccgt tttgcgttaa ctgaaagttg agataacgct cgccctggtt gctggcaaag
    50761 gttttttcac tccacggaaa ggcgtgggcg cgttgttcaa tgtggtaagc cgctggtaaa
    50821 tcagtcgttt cgagggaaga aatcgtgttc atatgtgcaa atttgttgcc ataacgcggc
    50881 gcgtgccgtt gggtttgccc gtaattcggt gagcgccggt gatgccacct gagcgccttc
    50941 caatgatagc ggttcgtcag tacccaaccg ccagctgttg cagcgactgc cttgcggcag
    51001 catcgcgatt ttttctggcg tcagttgcag cacttgatcg gggctgacgg ttaatgcgcg
    51061 cagaacatcg ctcactaaag gatcagtcag ggcgggaaga tcgtttgcca ccatcaccag
    51121 acggacgtgt gccgggctcg caatggcaat ctccccctgc aacgcgccag ggcgacgcag
    51181 cgaccactgg gtaatgccca gttgctgtaa ctgccagtct cgtcgggatg tcatagcgaa
    51241 tcgctcctgt tgtcaggggc gcaaatatag caaattcgtc gataccgcgc caacatatgg
    51301 ctataatcgc cgccagtatc aattgaggag cattccatgt ctgcatttac cccggcaagt
    51361 gaagtcttgc tgcgtcacag tgatgatttc gaacaaagcc gtattctgtt tgccggagac
    51421 ttacaggatg acctgcccgc gcgtttagat accgcggcca gccgtgctca tacccagcaa
    51481 ttccaccatt ggcaggtgtt aagctgccag atgggggata acgcccgttt tagtctggtc
    51541 gccacggcga atgatgtcgc agattgcgat acgctgattt actactggcc gaagaacaaa
    51601 ccggaagccc agttccagtt gatgaattta ctttctctgc tgccagtggg gacagatatt
    51661 tttgtcgttg gcgagaaccg cagcggcgtg cgcagcgctg agcagatgct ggcagattat
    51721 gcgccgttga ataaagtcga cagcgctcgt cgctgtggcc tctattttgg tcgtctggaa
    51781 aaacagccag tatttgatgc ggataaattc tggggcgaat acagcgtcga tggcctgacg
    51841 gtcaaaacgc tgcctggcgt gtttagccgc gacggtctgg atgtcggtag ccagttgctg
    51901 ctctcgacgt taactccgca cacgaaaggt aaagtgctgg atgtcggctg tggcgcgggt
    51961 gtactttcag ttgcctttgc gcgccattcg ccgaaaattc gtctcacctt gtgcgatgtc
    52021 tctgccccgg cggtagaagc cagccgcgca acacttgcag ccaacggtgt tgaaggtgaa
    52081 gtctttgcca gcaacgtctt ttccgaggtg aaaggttgtt ttgatatgat catctccaac
    52141 ccgccgttcc acgatgggat gcaaaccagc ctggatgcgg cgcaaacgct gattcgtggc
    52201 gcggtgcgtc atcttaatag cggcggcgag ctgcgaattg tagcgaacgc cttcctgcct
    52261 tacccggacg tgctggatga gacatttggc ttccatgaag ttatcgcgca aaccgggcgc
    52321 ttcaaggtat atcgcaccat tatgacccgc caggcgaaga aaggttgagt atctgatatc
    52381 tgtcgccgca taccgtgtcg gatgcggcgt gaacgcctta tccgacctac aaaacctgca
    52441 cgctagtttc ctgatggaca tttttccagc aattacacat caggcgataa ttaactattg
    52501 acgaaaagct gaaaaccact agaatgcgcc tccgtggtag caattctttt taagaattga
    52561 tggtatgcga aggtggcgga attggtagac gcgctagctt caggtgttag tgtccttacg
    52621 gacgtggggg ttcaagtccc ccccctcgca ccaaaaacca cgttgatatt gctcgcactg
    52681 ggcgaaggtg gcggaattgg tagacgcgct agcttcaggt gttagtgttc ttacggacgt
    52741 gggggttcaa gtcccccccc tcgcaccaaa cgaggcgata tcaaaaaaag taagatgact
    52801 gtgcgaaggt ggcggaattg gtagacgcgc tagcttcagg tgttagtgtc cttacggacg
    52861 tgggggttca agtccccccc ctcgcaccaa ttatctttac ttcctttctt gtttcttccc
    52921 tgttttgtca tccctgattc aaattcatta tcatcagcat gatgccagca ataagcgcca
    52981 ggcacgatgg tagcaaaatg taatgccgca actgtttgtg atacagcatc accggcgtca
    53041 atactaatcc catcagaatg acaatccgcc actgatccgg cgataaatcc gcatacatca
    53101 gccctgcaat cagcaatccc ggcagcaaca ctccccagcc actgcccagc gtacgttgca
    53161 acatggtttc atctctttca ttgataatga aaaccaatat catatgataa tttttatcat
    53221 ttgcaagcca gagaaatccc ttgctatcga gtaaacctat cgataggatt agcaatcatt
    53281 atcatttaga ttactatcac gattatggcc tatcgttccg caccgctcta tgaagatgtc
    53341 atctggcgaa cgcatctcca gccgcaggat gctgggcttg cacaagccgt acgggcgatg
    53401 atcgccaaac atcgtgaaca tttgctggag tttatccgcc tggatgaacc cgctccgctt
    53461 aacgccatga cgctggcgca atggtcatca ccgaatgcgc taagttctct actggcggtc
    53521 tattccgatc atatctatcg caaccaacca acgatgatcc gcgagaacaa accgctgatc
    53581 tcactatggg cacaatggta tatcggcctg atggtgccac cattaatgct ggcgctactg
    53641 acgcaggaaa aagcattaga tgtgtcgccg gaacatttcc atgctgagtt tcacgaaacc
    53701 ggacgcgtcg cctgtttctg ggtcgatgtg tgtgaagata aaaacgcaac accacattcg
    53761 ccgcagcagc gaatggaaac gttaatcagc caggcgctgg ttccggttgt gcaagcacta
    53821 gaagcgaccg gagagatcaa cggcaaactt atctggagta ataccggtta tttgatcaac
    53881 tggtatctca ctgagatgaa gcaactgctc ggcgaggcta ctgttgaatc gctgcgccat
    53941 gccctctttt ttgagaaaac gctcacgaac ggtgaagata atccactctg gcgtaccgtg
    54001 gtactgcgcg acggcctgct ggtgcgccgc acttgctgcc agcgttatcg cttaccggat
    54061 gtgcagcaat gtggctattg tacgctgaaa tgaaagcact gccggggaag taaacccggc
    54121 atcatgcgga ttaataggga tgcaacacat tacttgcttc gcaatgccgc atacataaca
    54181 gaatatctgc ggcctccaac aaccctgcat cagaactgac gcccagtttc gacatcacat
    54241 taaatttatg cgctcggatc gttttgatat tacgtttaag ctgctcggca atttgtgtca
    54301 ttgagtagcc acgcgacata aagcgcaata tttctctctc cgtcgggctt aacgtccggc
    54361 tttggttaat gtaccactga ttgttcagtc ggtcggtcgc ctgacgtaca ccatttaatg
    54421 acaaaaagag ttcctgatga aaaatctcca gcgttgacgc tttacttaat acaccgtcca
    54481 gcggtgatgg cgacaatgaa ccaatcagcc gagcttcaat atcatcatcc acaataacta
    54541 aacgccgggt acgcggaaac ttaatcgcca gctcagtcag gcaagataat ccctctctgc
    54601 gctcacttct catggcagaa aaagaaaaaa tgaccgccgc aaacgagatc cgcgacatgg
    54661 catcctgaaa tgactcctga ctggtatagg tatggagctt ataagggttg cccgcaaact
    54721 ttctgaataa actctcaata ccgatactac tcatgacgca tttttctatg agtgcgacat
    54781 ttctcttctt aattcggctg tgttccattc tgcggcatct ccataaacag aaggcaagtt
    54841 aagttctctt gcaccctgcg cgctatttat ccatgcatac atttcagcgt tgctgtgaat
    54901 tgccagtcga cgcatcgcac tgtttttttg ggcgctgata gttttattac ttttcttaag
    54961 caatgaagct atctggttga tgccccatcc tttacctaat agccgcagta ccttgcgctc
    55021 tgaaagagtg agaatgacgg agcgatagct aaaatcatga gtttcagtca ttgccggaga
    55081 ggtcagcatg gcgctgatac gctctgcatg cgtattgccg gaacgtacgg tcttgaccag
    55141 attctcaatg ggttcaacat cagacaataa cgtcgccgta gggcacatga gcagttctac
    55201 agcctgggaa taccaggagc gtgacaccat gaaaacccag tgaatttccc gatattgtga
    55261 gattaaagaa taataatgtt cacaaacaga acgtggatct tcactttcac cggctaaatc
    55321 agcaatgact aat
//
LOCUS       DAJTYG010000017        52952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTYG010000017 DAJTYG010000000
VERSION     DAJTYG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.017"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..374
                     /locus_tag="OO568_001084"
     CDS             <1..374
                     /locus_tag="OO568_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6640526.1"
                     /translation="IAGYYMKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQIL
                     NAIFLGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHAL
                     FWLAIGMLGIAMICLLFIKDI"
     gene            485..1408
                     /locus_tag="OO568_001085"
     CDS             485..1408
                     /locus_tag="OO568_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6640527.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVKGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1412..2230)
                     /gene="nlpA"
                     /locus_tag="OO568_001086"
     CDS             complement(1412..2230)
                     /gene="nlpA"
                     /locus_tag="OO568_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR6640528.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2452..2745
                     /locus_tag="OO568_001087"
     CDS             2452..2745
                     /locus_tag="OO568_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640529.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2786..3976)
                     /gene="nepI"
                     /locus_tag="OO568_001088"
     CDS             complement(2786..3976)
                     /gene="nepI"
                     /locus_tag="OO568_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR6640530.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4187..4639)
                     /locus_tag="OO568_001089"
     CDS             complement(4187..4639)
                     /locus_tag="OO568_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6640531.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4692..6026)
                     /gene="adeQ"
                     /locus_tag="OO568_001090"
                     /gene_synonym="yicO"
     CDS             complement(4692..6026)
                     /gene="adeQ"
                     /locus_tag="OO568_001090"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6640532.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVVLAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6201..7967
                     /gene="adeD"
                     /locus_tag="OO568_001091"
     CDS             6201..7967
                     /gene="adeD"
                     /locus_tag="OO568_001091"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6640533.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8013..9404)
                     /gene="uhpT"
                     /locus_tag="OO568_001092"
     CDS             complement(8013..9404)
                     /gene="uhpT"
                     /locus_tag="OO568_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6640534.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEVSEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9542..10861)
                     /gene="uhpC"
                     /locus_tag="OO568_001093"
     CDS             complement(9542..10861)
                     /gene="uhpC"
                     /locus_tag="OO568_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6640535.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(10871..12373)
                     /gene="uhpB"
                     /locus_tag="OO568_001094"
     CDS             complement(10871..12373)
                     /gene="uhpB"
                     /locus_tag="OO568_001094"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6640536.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12373..12963)
                     /gene="uhpA"
                     /locus_tag="OO568_001095"
     CDS             complement(12373..12963)
                     /gene="uhpA"
                     /locus_tag="OO568_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6640537.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13038..13328)
                     /gene="ilvN"
                     /locus_tag="OO568_001096"
     CDS             complement(13038..13328)
                     /gene="ilvN"
                     /locus_tag="OO568_001096"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6640538.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13332..15020)
                     /gene="ilvB"
                     /locus_tag="OO568_001097"
     CDS             complement(13332..15020)
                     /gene="ilvB"
                     /locus_tag="OO568_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6640539.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPSFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQLRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPVAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPLGAANTEMVGE"
     gene            complement(15126..15224)
                     /gene="ivbL"
                     /locus_tag="OO568_001098"
     CDS             complement(15126..15224)
                     /gene="ivbL"
                     /locus_tag="OO568_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6640540.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            15789..15878
                     /gene="tisB"
                     /locus_tag="OO568_001099"
     CDS             15789..15878
                     /gene="tisB"
                     /locus_tag="OO568_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6640541.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            16158..17342
                     /gene="emrD"
                     /locus_tag="OO568_001100"
     CDS             16158..17342
                     /gene="emrD"
                     /locus_tag="OO568_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6640542.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYKRTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17350..17847)
                     /locus_tag="OO568_001101"
     CDS             complement(17350..17847)
                     /locus_tag="OO568_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6640543.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMWVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17844..18206)
                     /locus_tag="OO568_001102"
     CDS             complement(17844..18206)
                     /locus_tag="OO568_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6640544.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18196..18543)
                     /locus_tag="OO568_001103"
     CDS             complement(18196..18543)
                     /locus_tag="OO568_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6640545.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            18652..19065
                     /locus_tag="OO568_001104"
     CDS             18652..19065
                     /locus_tag="OO568_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640546.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(19112..20554)
                     /locus_tag="OO568_001105"
     CDS             complement(19112..20554)
                     /locus_tag="OO568_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR6640547.1"
                     /translation="MVGCYSGKPLNTQNIDSLAAEGIRFNSAYTCSPVCTPARAGLFT
                     GIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWHLDGHDYFGTGECPPEWD
                     ADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETFTWAHRISNRAVDFLQQP
                     VRAEEPFLMVVSYDEPNHPFTCPVEYLEKYADFYYELGEKSQDDLANKPEHHRLWAQA
                     MLSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQRENTWVIYTSDHGEMMGAHK
                     LISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPTMMALADIEKPEILPGEN
                     ILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLVLNLFTSDELYDRRNDPN
                     EMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLRPWRKDARPRWMGAFRPR
                     PQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(20589..22304)
                     /locus_tag="OO568_001106"
                     /pseudo
     CDS             complement(20589..22304)
                     /locus_tag="OO568_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
     gene            22471..23364
                     /locus_tag="OO568_001107"
     CDS             22471..23364
                     /locus_tag="OO568_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6640548.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVLEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(23361..24175)
                     /locus_tag="OO568_001108"
                     /pseudo
     CDS             complement(23361..24175)
                     /locus_tag="OO568_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24175..25791)
                     /locus_tag="OO568_001109"
     CDS             complement(24175..25791)
                     /locus_tag="OO568_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6640549.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26086..26760
                     /locus_tag="OO568_001110"
     CDS             26086..26760
                     /locus_tag="OO568_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6640550.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNGLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(26799..28460)
                     /locus_tag="OO568_001111"
     CDS             complement(26799..28460)
                     /locus_tag="OO568_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6640551.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(28656..29084)
                     /gene="ibpB"
                     /locus_tag="OO568_001112"
     CDS             complement(28656..29084)
                     /gene="ibpB"
                     /locus_tag="OO568_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6640552.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(29196..29609)
                     /gene="ibpA"
                     /locus_tag="OO568_001113"
     CDS             complement(29196..29609)
                     /gene="ibpA"
                     /locus_tag="OO568_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6640553.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(29603..29675)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            29914..30246
                     /locus_tag="OO568_001114"
     CDS             29914..30246
                     /locus_tag="OO568_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6640554.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(30248..31385)
                     /locus_tag="OO568_001115"
                     /pseudo
     CDS             complement(30248..31385)
                     /locus_tag="OO568_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
     gene            31486..32504
                     /locus_tag="OO568_001116"
                     /pseudo
     CDS             31486..32504
                     /locus_tag="OO568_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(32465..33802)
                     /locus_tag="OO568_001117"
     CDS             complement(32465..33802)
                     /locus_tag="OO568_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6640555.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(33877..35025)
                     /gene="dgoD"
                     /locus_tag="OO568_001118"
                     /pseudo
     CDS             complement(33877..35025)
                     /gene="dgoD"
                     /locus_tag="OO568_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(35022..35639)
                     /gene="dgoA"
                     /locus_tag="OO568_001119"
     CDS             complement(35022..35639)
                     /gene="dgoA"
                     /locus_tag="OO568_001119"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6640556.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCAMATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(35623..36490)
                     /gene="dgoK"
                     /locus_tag="OO568_001120"
                     /pseudo
     CDS             complement(35623..36490)
                     /gene="dgoK"
                     /locus_tag="OO568_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
     gene            complement(36487..37176)
                     /gene="dgoR"
                     /locus_tag="OO568_001121"
     CDS             complement(36487..37176)
                     /gene="dgoR"
                     /locus_tag="OO568_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6640557.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVAPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            37454..38110
                     /locus_tag="OO568_001122"
     CDS             37454..38110
                     /locus_tag="OO568_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640558.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(38156..38968)
                     /gene="yidA"
                     /locus_tag="OO568_001123"
     CDS             complement(38156..38968)
                     /gene="yidA"
                     /locus_tag="OO568_001123"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6640559.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMGNAIPSVKEMANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(39082..39480)
                     /locus_tag="OO568_001124"
     CDS             complement(39082..39480)
                     /locus_tag="OO568_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6640560.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASCLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(39720..42134)
                     /gene="gyrB"
                     /locus_tag="OO568_001125"
     CDS             complement(39720..42134)
                     /gene="gyrB"
                     /locus_tag="OO568_001125"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6640561.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(42163..43236)
                     /gene="recF"
                     /locus_tag="OO568_001126"
     CDS             complement(42163..43236)
                     /gene="recF"
                     /locus_tag="OO568_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6640562.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(43236..44336)
                     /gene="dnaN"
                     /locus_tag="OO568_001127"
     CDS             complement(43236..44336)
                     /gene="dnaN"
                     /locus_tag="OO568_001127"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6640563.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(44341..45744)
                     /gene="dnaA"
                     /locus_tag="OO568_001128"
     CDS             complement(44341..45744)
                     /gene="dnaA"
                     /locus_tag="OO568_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6640564.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLSLYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            46041..46121
                     /locus_tag="OO568_001129"
     CDS             46041..46121
                     /locus_tag="OO568_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640565.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            46351..46491
                     /gene="rpmH"
                     /locus_tag="OO568_001130"
     CDS             46351..46491
                     /gene="rpmH"
                     /locus_tag="OO568_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6640566.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            46508..46867
                     /gene="rnpA"
                     /locus_tag="OO568_001131"
     CDS             46508..46867
                     /gene="rnpA"
                     /locus_tag="OO568_001131"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6640567.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            46831..47088
                     /gene="yidD"
                     /locus_tag="OO568_001132"
     CDS             46831..47088
                     /gene="yidD"
                     /locus_tag="OO568_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6640568.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            47091..48737
                     /gene="yidC"
                     /locus_tag="OO568_001133"
     CDS             47091..48737
                     /gene="yidC"
                     /locus_tag="OO568_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6640569.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHRFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            48843..50207
                     /gene="mnmE"
                     /locus_tag="OO568_001134"
     CDS             48843..50207
                     /gene="mnmE"
                     /locus_tag="OO568_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6640570.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            50449..50523
                     /gene="tnaC"
                     /locus_tag="OO568_001135"
     CDS             50449..50523
                     /gene="tnaC"
                     /locus_tag="OO568_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR6640571.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            50744..52159
                     /gene="tnaA"
                     /locus_tag="OO568_001136"
     CDS             50744..52159
                     /gene="tnaA"
                     /locus_tag="OO568_001136"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR6640572.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            52250..>52952
                     /gene="tnaB"
                     /locus_tag="OO568_001137"
     CDS             52250..>52952
                     /gene="tnaB"
                     /locus_tag="OO568_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR6640573.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWSAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGS"
BASE COUNT        13135 a        14120 c        13279 g        12418 t
ORIGIN      
        1 taatcgcagg ctattacatg aaacgtattg gcaagcgact attaatgctc attgctatcg
       61 tgagtggtat gtgtttttac gccagcgtac tcatggcgac gactccggcg gttgagctgg
      121 aattgcaaat tctaaatgcc atcttccttg gtattctctg tggtatcggc atgctttatt
      181 ttcaggactt gatgcctgaa aaaataggct ctgcgacaac gttatatgca aatacttcac
      241 gcgtcggctg gattatcgcc ggctctgttg acggaattat ggttgaaatc tggagctacc
      301 atgcgttgtt ctggctggcg atagggatgt tgggtattgc gatgatttgc ctgctgttta
      361 ttaaagatat ttagctcacc agtctgggct aagaaaaatc ggcgtggcgc aaacagagcc
      421 atcctcgcca tttttgctat catgcctgca tacataaacg acaaaacagt atgcagaggg
      481 aaaaatgggt tccaccagaa aggggatgct gaacgttctg attgccgccg tgttgtgggg
      541 aagttcaggg gtctgtgcgc aatacatcat ggagcaaagc cagatgtcgt cgcagttttt
      601 gactatgacg cgtttgatat tcgccggttt gattctgctg acgctgtcat ttgttcatgg
      661 cgataaaatc ttttctatta ttaacaatca caaagatgcc attagtctgc tgattttttc
      721 cgtggttggc gcgctaaccg tacagctcac ttttttgcta accatcgaaa aatcgaacgc
      781 agccacggca acggtgctgc aattcctctc accgacgatt atcgtcgcct ggttctcact
      841 ggtacgtaaa tcgcgcccgg gcattctggt tttctgcgct attttgacat cgctggtcgg
      901 gactttttta ttggtgacac acggtaatcc gacgtcatta tcgatctctc ctgccgcgtt
      961 attctggggc attgcctcgg catttgctgc tgcattctat accacctatc cctcaacgct
     1021 gattgcccgc tatggcacgt tgcctgtcgt cggctggagt atgctgatcg gcggtctgat
     1081 tctattgcct ttttatgcca gacaagggac aaactttgtc gttaaaggca gtttgatttt
     1141 ggcgtttttt tatttgatcg tcattggtac gtccctgaca tttagtctgt acctgaaagg
     1201 agcacaatta attggcggtc caaaagccag cattttgagc tgtgcagaac cattaagtag
     1261 cgcgctactc tctttgctgt tgctggggat cacgttcaca ttaccggact ggctgggaac
     1321 gctgctgatt ctgtcatcgg taattttgat ttcaatggat tcccgtcgcc gcgccagaaa
     1381 aataaatcgt ccggcgcggg atgagtgaga attaccagcc aggcaccgcg ccaccgttaa
     1441 agatggtttc tgccgcttta gcgacttcgg atgattgata agattgcaga aattccttca
     1501 cgttttctgc attcttatta tcttcccgtg ccaccaaaat attcacatac ggcgaatttt
     1561 tatcttcaat aaatacactg tcgtgcaccg gagaaagccc ggtctgctga atgtaagtgg
     1621 tgctgataat cgccacatca actttgggat catccagtac gcgcgggagc tgcgccccct
     1681 cgagttccat aatctgcaaa tgacgaggat tatcggttat atccagcgcg gtaggtaata
     1741 acccttttcc ctctttcaga gtaattagct tctctttttg caacagtaaa agcgcacgac
     1801 caaggttggt cgggtcgttg gggatcgcca ctgtcgcgcc ttcttttatt tgcgccacgg
     1861 ttttgatttt tttggaataa cccgccatgg ggaagacaaa cgtatttccc accgctacca
     1921 gtttataacc atgcgcctga ttatcttgtt caagaaaggg tcgatgctgg aagacgttcg
     1981 catcaagttc gccatgattt gtcgcatcgt tcggtagcaa cgaaccgcta aatcctacca
     2041 gttgaacatc gaggccatat ttctcttttg ccaccttttt agcgacttcc gcgacatctt
     2101 gttctgcgcc atttataacg ccaactttaa tgtgttttgc atcgctgcta ctctggtcgc
     2161 aacctgccag cagaattccg gccagcaata atgcggcccc tgtccgtaga tgatgtgttg
     2221 tcagtttcat ttttttatcc ttttattaat gattacctga ctatagcggt aaggtcgctg
     2281 cggtttaaaa aacgaaacgc tatcgataag aataaaaagg aataaaagtg gaatataaag
     2341 tctggcactt cttactgacc acgcaagcgc gtttcgtaca gcatgatgag agcgatgaaa
     2401 gcaaactaca cttatgcttc attcgataca catttgtaaa aggttgaatc catgaaacca
     2461 acgacactac tgcttatttt cacttttttc gctatgccag gcatcgtata cgccgagtcg
     2521 ccttttagct cactgcagtc cgcaaaagag aaaaccacgg tattacaaga tttacgtaaa
     2581 atttgcacgc cacaggcgtc attatcagat gaagcgtggg aaaagttaat gttgtcagat
     2641 gaaaacaata aacaacatat acgcgaggcc atcgtggcga tggaacgtaa taatcagagc
     2701 aattattggg aagcgttggg taaggtagaa tgcccggata tgtaagaaca gcaacaacta
     2761 ctgaccaacg cttctttacc tgatatcagg atttcttcat tttcaccttt gcagtaacca
     2821 acaatgcagt cagcaacatc aatgtgccgg acaacatcaa cggcgaagtc agaccaatat
     2881 tatccagcgc ataaccgccg attgccgcgc cacaggtatt agcaagctga ataaccgcca
     2941 cctgaataga cccggctttt tctgcctgat cggccagcga gcgggtgatc cacgttgacc
     3001 agccgacggg aaccaatgca aaagttagcc cccagataat cgccacgccg gtagcaacga
     3061 ttttatcgct tccccacaac gtcagtacca acgcactcac agccagtatt aacggcgcgc
     3121 ctgctaaggc cagttttacc gaacgtttaa gaatgaacga cgaaagcgac gtaccaataa
     3181 agctggcgat accaaaactc aacagcacca gcgttaagcc atccacgccg aatcccgcca
     3241 ggttcatata caccgggcga atatacgtga agaaagcaaa ctgcccggcg aaagacatga
     3301 agatggcgat catccctgcc atcacacccg gacgttgtaa taagcggaaa gtattttgtt
     3361 tctgatgcga gggttcgcct ggcagtgaag gcaatgattt gataatccag aaaatacaca
     3421 gcacgcccat caccgccgcc gcattaaaga cattgcgcca accgataagc tcgcctaaaa
     3481 aactgcccaa cggcgcggca atcaccagcg caatagaaac cgcgccgaag atcaccgaca
     3541 gcgccttcgg caccgtacgc ggcggtacca gacgcatggt cagcgacgcc gacatcgccc
     3601 agaacccgcc cagcgccagc cccagacagg cacgaccgat taaaagcaaa ctgaatgagt
     3661 tagcaaagga aaccagcaag caggagagcg tcagcaaaac ggcaaacaaa ataacaacgt
     3721 agcggcggtc agtggcctga attgtctggg taataaacaa actggcaaac attgccacaa
     3781 aggcggtcac ggtcaccgat tgcccggcaa ccccttccga aatgcctaaa tcctgggcca
     3841 ttggcgtcaa caaactgacg ggcaaaaact caacgataat cagacaggcg acacaaaacg
     3901 ccaccgagaa aacggctgac cagttcggtc gggtgatggc atccgcgccg cggttttcgg
     3961 caataaattc actcatggtg ttacccgtgg catgtttcag gggaaaagcc gaccacttta
     4021 acattgaaat gtgtgacgca tttaacgttt ttgcaacttt tacggtgttg cttgagctgg
     4081 agttaagatc gaaacggaac agggcaaaga ttaccggatg cggcgtgaac gtcttatcca
     4141 cccgacaggt caggcgcggt ctgtaggctg ataagacgaa acggtttcaa tgcaaccagt
     4201 gaacgccctc ggcaggctga aagaacgcgt tatagcgcat ctgaaaagca ttaatatcct
     4261 gcatttcagg ttcggtttca cgcagaaaac ccagcgccag cctgacctga gcatcggtaa
     4321 tcggcgcgga aaggcgggct ttttgtaaaa tccggtgcca ttgcagcagg ttctgttcgc
     4381 tatcatcgac aatgacaatt tgccagataa gcaacacctg cgcggcgttt tgcaggtgcg
     4441 actcatccgg gcgatgcaaa taacgtaaaa attcgtcacc ggctgacttt cccatttgat
     4501 cgaccatcga ctccacgggt acgacatcga tattcagccg atcgctgaga cggcgaatcg
     4561 cttttctggc cccggatgtc agcacccgaa aaccaaccac aaacactacc gccagcgtgg
     4621 cgagcattat ccagatcatg agttctcctg cgtaatcaga actcatgata tctggaaacc
     4681 ctcgccagtt actaatccac cagaataatc ttcaatgcga acagagctgc gaccaccacc
     4741 acacacaggt tcagatcgcg ccagcgcccg gtgcacactt tcatgatgca gtacgacata
     4801 aagccgagtg caatcccttc ggtgatcgag aaagtaaagg gcatcatcac cgtggtaata
     4861 aacgcaggca ccgattcggt aaaatcatcc cagttaacgc gcgccaggct cgaagtcatc
     4921 agcacgccaa caaagattaa cgctccagcg gttgcgtaag gaggaaccat cgccaccagc
     4981 ggtgagaaga acataactaa caggaacata acgccgacca cgacagccgt tagccccgtg
     5041 cgaccaccga ctgccacacc agaagtactt tcaatatagg cagtaacaga cgaggtaccg
     5101 ataaatgcgc ctgccaccga actgacgcta tcaacataca gcgccttatt catattgggg
     5161 aatttaccgt tgctatctat taagcccgct ttatcagtta caccaattaa tgttcctgat
     5221 gaatcaaata ggttgatcag cataaaggag aaaatgatac cggcgagttc aagtgttaac
     5281 gcgccgctca aatctacttc accaatgacg ccgctaatat caggcggaat ggaatagacg
     5341 ccgctaaaat gaacatcacc gaaaaataat ccacagcaag acgtcaccac aatagaaacc
     5401 agcaccgcgg catgaaaatg acgtgatgac aacacggtta taataaaaaa ccctaaaata
     5461 cctaacaaca cgccgtgaga acttaaatcg ccaatcatca ccagcgtgtc tttattggcg
     5521 acaataacac cagtgttttt taatcccatt aaggcaataa ataatccgat tccactggtg
     5581 ataccaatac gtaaacttaa tggaatgttg gagatcatcc agtaccggat acgaaacagc
     5641 gtgagcaaaa atagcccaat tgcgccccag aatatcgcgc ccatcccggt ctgccaggag
     5701 atccccatcg cccccacgac cacgaacgca aagaaggcgt tcagccccat tgccggagcc
     5761 agcaccacgg gtaagttagc aaatatcccc atcgcaatac tgccgatacc ggcaatcaaa
     5821 caggtggtaa caaacaccac tttcgggtcc atttgtgccg cgccgaggat ttgcgggttc
     5881 acaaaaacaa tgtacaccat ggttaaaaaa gtggtcatcc ccgcaatcaa ttctgtgcgg
     5941 acggtggtcc catgctgagg tagtttaaat aatcgcgaaa gcgtccctga ttcattactc
     6001 acgtaatcgg tattgtcatt attcattttt ttgtccattg gaggagattt aatcccttgc
     6061 ttttgtaaaa caaatgacaa caacggttca gtgataatta tcacatttca attgcacatt
     6121 aatagatatt ctttaataat ctcgcgacgt ttctttatga taaataataa tcaaattgat
     6181 aaaatcaaaa tgagaaaaat atgaataatt ccattaacca taaatttcat cacattagcc
     6241 gggctgaata ccaggaattg ttagccgttt cccgtggcga cgctgttgcc gattatatta
     6301 ttgataatgt ctctattctc gacctgatca atgccggaga aatttccggc ccaattgtga
     6361 ttaaaggacg ttacattgcc ggtgttggcg cagaatacgc tgatactccg gctttgcagc
     6421 ggattgatgc tcgcggcgca acggcggtgc cagggtttat tgatgctcac ctgcatattg
     6481 aatccagcat gatgacgccg gtcacttttg aaaccgctac cctgccgcgc ggcctgacga
     6541 ccgttatttg cgaccctcat gaaatcgtca acgtgatggg agaagccgga ttcgcctggt
     6601 ttgcccgctg tgccgaacag gcaaggcaaa accagtactt acaggtcagc tcttgcgtgc
     6661 ccgctctgga aggctgcgat gttaacggtg ccagttttac ccttgaacag atgctcgcct
     6721 ggcgggacca tccgcaggtt accggccttg cagaaatgat ggactaccct ggcgtaatta
     6781 acgggcagaa tgcgctgctc gataaactcg atgcatttcg ctacctgacg ctggacggtc
     6841 actgtccggg tttgggtggc aaagaactta acgcctatat tgctgcgggt attgaaaact
     6901 gccacgaaag ttatcagctg gaagaaggac gccggaaatt acaactcggc atgtcgttga
     6961 tgatccgcga agggtccgct gcccgcaatc tcaacgcact ggcaccgttg atcaacgaat
     7021 ttaacagccc gcaatgcatg ctctgtaccg atgaccgtaa cccgtgggag atcgcccatg
     7081 aagggcatat cgatgcctta attcgccgcc tgatcgaaca acacaatgtg ccgctgcatg
     7141 tggcatatcg cgtcgccagc tggtcgacgg cgcgccactt tggtctgaat cacctcggct
     7201 tactggcacc cggcaagcag gccgatatcg tcctgttgag cgatgcgcgt aaggtcacgg
     7261 tgcagcaggt actggtgaaa ggcgaaccga ttgatgcgca aaccttacag gcggaagagt
     7321 cggcgagact ggcgctgtct gccccgccat atggcaatac cattgcccgc cagccagttt
     7381 ccgccagcga ctttgccctg caattcacgc ccggaaaacg ctatcgggtc attgacgtca
     7441 tccataacga attgattacg cactcccgct ccagcgtcta cagcgaaaat ggttttgatc
     7501 gcgatgatgt gtgctttatt gccgtacttg agcgttacgg gcaacggctg gctccggcct
     7561 gtggtttgct cggcggcttt ggcctgaatg aaggcgcgct ggctgcgaca gtcagccatg
     7621 acagccataa tattgtggtg atcggtcgca gtgccgaaga gatggcgctg gcggtcaatc
     7681 aggtgattca ggatggcggc gggctgtgcg tggtacgtaa cggccaagta caaagtcatc
     7741 tgccgttacc cattgccggg ctgatgagca ccgacacggc gcagtcgctg gcggaacaaa
     7801 ttgacgcctt gaaagccgcc gcccgtgaat gcggtccgtt acccgatgag ccgtttattc
     7861 agatggcgtt tctttctctg ccagtgatcc ccgcgctaaa actaaccagt caggggctat
     7921 ttgatggcga gaagtttgcc ttcactacgc tggaagtcac ggaataataa aaaaagcccg
     7981 gcgtcatgcc gggcaaaagt caccagttac gtttatgcca ctgtcaactg ctgaattttt
     8041 ttctcgcggc ggattttgcg ttcttccatt accgctacta tcgccatcag gcagatacaa
     8101 ccaatcgcgg cgatatccag cgcggcgaag gtgcctgccc agccggtaag gccgaatacc
     8161 ggcgtcccat cggcaatcat tcccagacct aacttggcaa agctgtcacc aatcaggtaa
     8221 gcaaaggtgc ctttaatacc atcggcagcg ccaatcgctt ttttaggtac aaagccaaca
     8281 gcagccacac caatcaacaa ttgcgggcca aagaccagga aacccaacgc aaagagagaa
     8341 gccagataga tatattcgtt actggcatgt tgatacacac cgagcgtggc gataatcagc
     8401 gccagcgcga tgcaggccac caggccacgg cgaccgttcg ccaggtcaga gagccagccc
     8461 cacagcagcg taccgaccag cgcaccagct tcaaacagcg taaagccctg aatcgccacc
     8521 gctttagaga gtttcagttc ctggaacgcg tatacggttg accactggtc gataccaata
     8581 cgtaccacat agaggaaaat gttggcgaag cacagcagcc agatcacttt gtttttcagc
     8641 acatactcaa caaagatctg ccacttggtc atatcggtag attctgtctc tttgtcctct
     8701 tcgctgacct cctcgccgaa cagttcttca gctttgccga ggccataaga ttccggggag
     8761 tcgctaccgt aacgcaggcc gataaaaccg acaatcagcg cgataatcga cgggaagata
     8821 aacatgccga tgacatggcc atcgaacagg taatttgccc cgaacagcgc cacacctgct
     8881 gcgcctgcac cgccaaggtt gtgagaaata ttccagaaac cgaggaatgt cccgcgttta
     8941 cgacgcggcg tccatttggt gatggtggag taactgcacg aaccgccggt actctggaaa
     9001 aagccgctta aggcgtagaa ggcaatcatc aggaacaggc taaccgagcc gctgcccata
     9061 ctggcactga agcccagcat acaaatggca gagaggataa gcatgaacgg caggaattgt
     9121 ttggtgtttt tgccgtcggc gtagtatgaa accaacgttt tacccacgcc ataagtgatg
     9181 gagaaaccca ggccgatcat ccccagctgc gtcatgctca acccgtaggt cgaaatcata
     9241 tcgttctgcg cgatgttaaa gttcttgcga atcaggtaca tcgtcaggta gccgataaag
     9301 accaccaggt aggattgcat gaacggtttg aaccacattt tgcgccgcac ttcgagcgga
     9361 aggtccaggg tcggcttgcg aacctggttt aggaaagcca gcatgggtta ctcctgaaat
     9421 gaatacctgc gtgaggcatg cattgtaaaa atcagcagca aatcaggcct gagatagcgt
     9481 ccaagaaaaa cctggaaaat ttcttagttt tgccggatat gaagtgaaaa ggtgagatgc
     9541 atcacgcttc acgcggtgtc tgggcgttca aaaagggcag taacagcagt gcggaaatcc
     9601 cggcggcgat agagataacc acaaaaaatc cgctccagtg ccaggtatcg agcactttcg
     9661 ccagcggcca accagcaagc gacgccccca gataagcaaa caagccgaca aaccccgtcg
     9721 ccgcccctgc cgcctctttg tgggaacact ctgccgccgc cataccaatt aacatctgcg
     9781 ggccaaagac aaaaaaacca atggtgaaga agcaggttgc ctgcatcacg tagctggcaa
     9841 atggcatcag ccacagcgag ccgactgaaa gcaaaattcc ggcggcgaaa atcaaattca
     9901 tcggcccacg gttgccgtta aacaatttgt ccgagcccca accggctacc agcgcaccga
     9961 taaatccgcc cagttcaaac atcgtcactg ccgtattcgc cgtgaccaga tcgacgccca
    10021 gcgtctcgga catatacaaa ttgccccagt cgttgatcgc cgcccggacc acatagacca
    10081 gcacatagca aaacgaaagc agccagatat acggattcag caacacatat ttggtgagga
    10141 tctctttacg cgtcaaccct gccccttctt gttgttgagc aatttccagc gcgtcgtgcc
    10201 gccattcacc gaccgccggt aaacctaacg cctgcgggcg atcgcgtagc cgccagcaga
    10261 gaaaaatccc cacgactatc gccatacaac cagcaatcat catcccggca cgccagccgt
    10321 aatgcagcgc gctcgctgcc atcacaatgg gaatgagtgc gccgccgacg ttatgcgccg
    10381 tgttccataa tgcccaccaa ccgccgcgct cggtacgtga ataccaggcc gttaacagac
    10441 gcgcacacac cggtgaaccc cagccctgga aaaaggcgtt cagcacccag agcacggcaa
    10501 acgcccatag cgacgtcgag aagccaaaca gaatgttcat gatgcccgtg gcgataagcc
    10561 ctatccccat aaaataacgg gcatttgagc gatcgctgac aatgccggag acaaacttcg
    10621 acacgccgta agtaatgtaa aacagggtcg ctaacaggcc gatatcgcta cggctgagca
    10681 cgccgttagc aaggatttct ggtaccgcgg cgttaaaact tttccgcgtg aagtaaaaca
    10741 gcgcgtaacc cagccagatg gtcagcagaa tatgccgacg ccagtagcga taacgggcat
    10801 caatttcatg tttatcagtc attaatggcg catcggcagg cgctttcaga aacggcaaca
    10861 tcatcgaacc ttagacataa cgttgaggta gagaaacgct gacacgcgtg ccgtgcagac
    10921 aggaaatgtg taatgtgccg cccagcgccg ttacgcgctc gcgcattccg gtgaggccaa
    10981 aaccttgttg cccggaaccc ggcggcaaac cgctgccatc gtcttcaatc accagcatca
    11041 accgttcatc ctgctgccag ccttgcagcg taaccgcgct ggcatcggca tgtttcacaa
    11101 tgttgttcag cccttcctgg cagacacgaa acagcgtcac gcgctggttt tcgcttaatg
    11161 ctgattcatc gatacgccat tcgagatggc tgacaatgcc gcgcccttcc agctccattt
    11221 cccgcatcag tgagcggatg gcctgctcca gggtgagatc atccagctgg cgcggacgta
    11281 accgtcctaa caaacggcgc accgcgtcgt aaacgcccag cgacagttgt tcgatgagct
    11341 gcccgctctg cttcacactg gcgttatctg ccgccagccg ctgaacaatg cccgcctgag
    11401 tacgaatagc agtgatggtc tgaccgatat cgtcgtgcag ctcacgcgcc acgtcgcggc
    11461 gcacgctctc ttcggtttct aacaaacgtt cagccagatg ctgattgcgc gccagttcct
    11521 tttgtagcga ctggttaagt tcacgcaacc gctggatgcc tgcgcccagt aacaaccctg
    11581 tcagactttg caccagcaac gagagtaata aatccaccag atgatcgcgc caggtttgac
    11641 tggcgatcag cgcgatggcg ttcatcaacg tcgcaatcag cgctccttgc cagccatagt
    11701 gccaggccag cgcgataatc ggcagcgcca gacagaatgg cgtaaagcgc gacagttcgt
    11761 ccggcaatcc caactggagc cagagactga taacaaacag cagcaagtac cagaccagat
    11821 gccgcccgcg ccagttgatg ggctgagaaa ccagtgacgg gccgagcggc agccaggtgt
    11881 tattggcgag atagtgccag aacaccagac atatcggggc cagcgtcaga ccgccagtta
    11941 aagtcagcaa cagcgcattc cacgactctt tgccgtgcca aagccagggc agcgactgca
    12001 acaacgccgc cgccgttaac gccgcaccct gtaacagcaa ggtgcgccag tcacgctgat
    12061 ggcgatagcg cgagatcagc gctacgggca gtaacgtcag taaactaccg atcatcaata
    12121 acggaaaatg ggttaaaccg accgcctgcg ttagccagta aatcagcagc cactccgcac
    12181 ccagcaatac gggccagtat ccgcgcgggc attgcagcat tagccccaga cgcagaccaa
    12241 acggaaataa cagcaccgcc atatcagggc gctcaaccag atgcaggctg atactccata
    12301 ggcaaaacca tgcggcagag aagataaaaa agcaggcaat aacggtaatt aagcgggaga
    12361 acaacgtctt catcaccagc catcaaacat gcggcgcgcc agctctacgt cgttactgac
    12421 gcccagtttt tccatcagat tggcgcgatg gacgtgtacc gttttcggtg acaagcccag
    12481 ttcggcggca atctctttca ccgccattcc ttgcgccagt ttttccgcca cctggcgttc
    12541 acgtttggtc agcgggtcct gacgaccgga tgccagttta atggcaatat ccggcgtcag
    12601 ataacagccg cccgtggcaa ccgtatgcac cgcagcaatg agttcatccg ggctacagcg
    12661 tttggaaaga aagccgcgtg cccccgcgtt aagcgcctgc tcaaccagcg caggactgtc
    12721 gtgaacggag agcataatcg tcgccatacc tttcggcagc tggcttagca gctccagacc
    12781 ggagatatcg ggcatggaga tatcgcaaat acacacctgc acaccgcgcc ccggcagccc
    12841 cgccagcgcc tcgcgccccg aaccaaactc ggcaactacc tgcaaatcag gttccagccc
    12901 cagcagctgc gcaaagccgg agcggacgat gaggtgatcg tctataaggg caacggtgat
    12961 catggtcttg tcctggcggg taaaaaaaaa cgcgcttacc ttaacgataa gcgcgatgtt
    13021 gttcaagcct tgagcggtta ctgaaaaaac accgcgatct tgttaaacat cgtcggatcg
    13081 gactgattac gctgcacttt cacgacatct tccagcttat cgatttggct tatcatctgc
    13141 tccagacgct ggtcgtcatt gaccagtagc cagatatggc ttttgtcgct gtcctgaatc
    13201 ggcagacaaa gaatgccttc aacgttaaaa gcgcggcggg caaaaaggcc acaaacgtgg
    13261 gtcattacgc ccggatggtt gcgaacggtg agctccagaa ttacgttgtt atgagttgtg
    13321 ttttgcatgg cttattcccc caccatttca gtattcgccg cacctagcgg caccatcggg
    13381 taaacttttt cttcggcatc aatgcgcaca tggatcagcg ccgggccagg gcgattgatt
    13441 atttcctgca atgcagcctg cggatcggct tcgttattca aatcacaggt ttcgaggccg
    13501 aatccggcgg caatctgcat aaagttgatt ttgcccggat aggtggcggc aaaaacgcct
    13561 tgctcgtaga acagactctg ttgctgatgc accagcccca gcgcttcgtt gttcatcaga
    13621 atgattttga catccagctg attttcactg gcggtcgcca tctcctgaat attcatcatc
    13681 aggctgccgt cgccggagaa acacaacact ttgcgatccg ggttcgccag cgccgcgcca
    13741 atcgccacag gcaggccaaa acccatcgtg cccagcccac cggaggtcag ccactggcgt
    13801 gggcgattga gcggataagc ttgcgcggtc cacatctgat gctgacccac atcggtggtg
    13861 ataatcgcat tgtcatcgac acaggcggca acggcgttga tcaggccgta atggcttaat
    13921 ggatcgcacg ctttcgggat tggacacggg aattcgcgct gcaaatccgc taccaactgg
    13981 tgccactctg cacgcagttg cgcttccacc tgcgggatca attgcgccag cacgtcatca
    14041 acatccgcct gaatcgccac atgcggctgc ttgattttac ccagctctgc gcgatcgata
    14101 tcgacatgaa taattttggc attcggacag aactgctcgg ttttgccaat cgcccggtca
    14161 tcaaaacgcg caccgagcac aatcaacaaa tccgcctcct gcaaaatata gttggtactg
    14221 cgcacgccgt gcatccccag catacccagc gacaacggat gcgcttttgg cagcatgccc
    14281 agcgccatta aagtcatggt ggtaggcagt tgcgctttct ccgccagttc acgcacccgc
    14341 gcaggcgcat tgatcacacc accacccaga taaagcaccg ggcgtttggc agcgttaatc
    14401 attgtagctg cgtcacgaat gctttcttcg ctaaaggagg gtgcagcggc tttttctgcc
    14461 accgcgggct gagcttcaat ctcaaaaacc gccgtttgta catccttagg aatgtctatc
    14521 cacaccgggc tagggcggcc tgattgcgca atgcggaagg catcgctcat gacctgtggg
    14581 agctcttcga tatgtctgac cagatagttg tgtttggtga tggggataga gatgccgtag
    14641 gtgtccactt cctggaaggc gtcggtgccg atcatcgagg cgggaacctg accagtgatg
    14701 caaatcagcg ggatggagtc cagccgcgca tcagcaatgg cggtcaccag gttagtcgca
    14761 cccggcccgc tacaggccat acagaccgcc ggtttaccgt cggtgcgcgc cattccctga
    14821 gcgataaacc ccgcgccctg ttcatggcga gccagaatat ggcggatttg cgtgctttgg
    14881 cttaaggcgt cgtaaacagg caggatagaa ccgcccggaa tgcccgtcac aatcttaatg
    14941 ccctgctgtt ccaggaaatg aacgataaat tctgcgccgg taaagcgctt acgcgtcgat
    15001 gttgtgcccg aacttgccat gctccagtcc ttttcttctg ggccgacttt ccgggaggtg
    15061 cttaaacgaa aaaccccgcc cggtttgcgc cggcggggtt ttggaatcgt gtgttgttcc
    15121 agtccctacg gcgcattgcc gacgaccacc accacacgca cgacgaccac tgcggcggat
    15181 ggcgcagttg gtagtagttt tgcgttgaac atggaagtag tcattgggga ccttgttggt
    15241 tttgtgttta acaatattta tacaagcaca gctttacagg ggagacaatg gaaaattttt
    15301 cagcaaggga aaattgaggg gttgatcacg ttttgtactg aattgcagat aacaaaaaac
    15361 cccgccggag cgaggtttcg tcagccgcct gcggctggta accgcaaagc acactgtatt
    15421 atgtcaacac tgaaagtata cgtgttccgc gcagaacgcg caatttcggc acgaattttg
    15481 acgtatttag tgcatagttg agtatcgttc acagtttgcg ttttgtccaa atattactgt
    15541 ttatttatac agtaaacttc tataatatca ctgttcgcaa tgtgttatgc gggggccgca
    15601 tcgttacccg gcgcactaag tcctggctga aacgggtggt gccgtcagcg ccttaacccc
    15661 gcgtgagcac actgtgttat gtcaacaagc acaacgtttc tccttgagat accgcgtgca
    15721 caacagctgg caacaggcag cggaaaggta cgtcagctgg cagtgctcct gaaccacagg
    15781 agacgcgtat gagcctggtg gatatcgcca ttcttatcct caaactcatt gttgcagcac
    15841 tgcaactgct tgatgctgtt ctgaaatacc tgaagtaatt cagattcaag tcgcaccaaa
    15901 ggggagcggg taaccgctcc ccttttatat ttagcgtgcg ggttggagtc ggatgcgatg
    15961 ctgacgcatc ttatccgccc taccatccct cccggcaaca tttattgccg cttttgttta
    16021 catattctgc cgctaaacaa ttccccattc ctggcgtata tctggctaac attcatcaat
    16081 gtgatagatt cctctcccgc atttatggga atgcgtagtg acttattcta attattttta
    16141 taaaagcatc cgtgataatg aaaaggcaaa gaaacgtcaa tttgttattg atgttggtat
    16201 tactcgtggc cgtcggtcag atggcgcaaa ccatttatat tccagctatt gccgatatgg
    16261 cgcgcgatct caacgtccgt gaaggggcgg tgcagagcgt aatgggcgct tatctgctga
    16321 cttacggtgt ctcacagctg ttttatggcc cgatatccga ccgtgtcggt cgccgaccag
    16381 tgatcctcgt cggaatgtcc atttttatgc tggcaacgct ggtcgcggtc acgacctcca
    16441 gtttgacggt attgattgcc gccagcgcga tgcaggggat gggcaccggc gttggcggcg
    16501 taatggcgcg tactttgccg cgtgatttat ataaacggac acagttgcgc catgctaaca
    16561 gcctgttaaa catggggatt ctcgtcagtc cgttgctcgc accgctaatc ggcggtctgc
    16621 tggatacgat gtggaactgg cgcgcctgtt atctcttttt gttggttctt tgtgctggtg
    16681 tgaccttcag tatggcccgc tggatgccgg aaacgcgtcc ggtcgatgca ccgcgcacgc
    16741 gcctgcttac cagttataaa acgcttttcg gtaacagcgg ttttaactgt tatttgctga
    16801 tgctgattgg cggtctggcc gggattgctg cctttgaagc ctgctccggc gtgctgatgg
    16861 gcgcggtgtt agggctgagc agtatgacgg tcagtatttt gtttattctg ccgattccgg
    16921 cggcattttt tggggcatgg tttgccgggc gtcctaataa acgcttctca acgttgatgt
    16981 ggcagtcggt tatctgctgc ctgctggctg gcttgctgat gtggatcccc gactggtttg
    17041 gcgtgatgaa tgtctggacg ctgctcgttc ccgccgcgct gttctttttc ggtgccggga
    17101 tgctgtttcc gctggcgacc agcggcgcga tggagccgtt ccccttcctg gcgggcacgg
    17161 ctggtgcgct ggtcggcggt ctgcaaaaca ttggttccgg cgtgctggcg tcgctctctg
    17221 cgatgttgcc gcaaaccggt cagggcagcc tggggttgtt gatgacctta atgggattgt
    17281 tgatcgtgct gtgctggctg ccgctggcga cgcggatgtc gcatcagggg cagcccgttt
    17341 aagcgcacgt caccgcagca tcgtcatcag ctccatggga gaacgatgct gctttatcag
    17401 atcacgcatc acccacatat gcggtgcgga gtaagaataa aacgcctgat agcccgcaca
    17461 aagcacgctg tctggcgtct cctgccgagc gtgcgggcac aggcgatgcc agtcgcacgc
    17521 ctggcagtca cggcgcggtt gcgggttttc gctgccacac catttttgta aggtggcgtc
    17581 aaaaagctca atcgagatac gactgatatc tgcccgcacc caacactcaa acagggcaat
    17641 taaaaatttc ccccacagct ctgctgtcac cgaagcggcc gtcagatcgc cccggtcatc
    17701 aaattcaaga tggggaatga gctgtacgga gcggatgccc cgctgtttga aattgtgata
    17761 aaaatcggca ggttgcagag catccttgta ctctacaacc agcttatgcc tgtcctcttc
    17821 agcatcaatg acctgacttc ctgtcatgcg actctctcaa taaatacgat aagttgatgt
    17881 atatgcgtta cagcaaacag tattgcgaga gataacaccg cgagggagat caaaaatttg
    17941 tcacggacta cgtgaaattg ggaaaaatcg ctattcgtga catccattaa attgcgatta
    18001 cgggtgtagt gccagaggat cagcgccacg atggcgagga tgccaatcga aatccagaat
    18061 aacatgcccg cctggtgcca gttgtgtttg atagccaacg ccatcaacgc gccgtagccc
    18121 agcatggtac gaaaccacgc cagtgatgtg cgctccggct gcagccctgg gtcggcaatg
    18181 cgtctggctt tacggctatc cggcatacaa caccagtccc ataacgatca ccgccacaac
    18241 catcaatatt aagctgatga ttaataagct gttggtgtag ggcaggtcct ctttcagacg
    18301 catcgctttt tcattgcgta accagcgcag gtagccatac attgctaacc cgccggaaaa
    18361 cagacacaac aacaacgcca gcagttcacg aatgacgggc gtggcgaaat ctggcgcaag
    18421 ttgatcaagc ccaacccccg ccgccagaaa tcctagtgcg gtacggatcc acgccagaaa
    18481 agtacgctca ttagccagcg agaagcggta atcgggcgct tctccaaggc gggaaatttt
    18541 cattgttgct cctttgcaat ttcactaacg ctctatcttt agcggttttt tttaagaatt
    18601 tcgtccgccc gtttcctgat atgcttttgc catccgctac aaggaggtca gatgggtatc
    18661 atagcgcaaa ataaaatcag ttcattgggc atgctgttcg gtgccatcgc tttaatgatg
    18721 gggattatcc acttttcatt cggccctttt tccgcaccac cgccaacgct tgaaagcatc
    18781 gtggcggaca aaacggctga aattaaaaga ggtcttctcg ccggaataaa aggcgaaaaa
    18841 attaccactg tggagaaaaa agaggacatg gatatcgata aaatcctcga tcaaagcggt
    18901 atcgcactgg ccattgctgc gctactttgc gcatttattg gcggtatgcg taaggaaaat
    18961 cgctggggca ttcgcggcgc gctggttttt ggtatcggca tcgtgtgcag cattttgctg
    19021 atattcctga ttttttcctt cctgaccggc ggttcgctgg tttaagcagc ctgaataatg
    19081 cccgccgggt gatcatcagc gggcattatc gtcagaattt ctgttttttc tcctccacct
    19141 tcactccttg cgtcggtagg ccggtgtcat agtcgcgtac cactggcgaa tagccatctt
    19201 gtgggcgtgg gcgaaacgcc cccatccagc gcggtcgtgc atctttacgc cacggacgca
    19261 gactccattg gtaactgcgg aacggatcgc gaattttgtc catgtaatcc aataaggcgt
    19321 catgcatttt gctgcgaacg tctgcaaaac ggatatcatc gatcaggtta tgcatttcat
    19381 ttgggtcatt acggcgatcg taaagttcat cactggtgaa gaggttgagc accagtttaa
    19441 agtcatccgt cacccagcaa cgcaccggaa taaaaccgcc aaagctgtca tgctcaatct
    19501 cgtagcggtt aaattccacc atcacgccgc gtggctcttt cacggcaagg atattttccc
    19561 ccggcagaat ctctggtttt tcaatatctg ccagcgccat cattgtcggc agtaaatcaa
    19621 tatgactgac tggcgtatcg acctgtcgcc gctccccttg cggcgaacgg atgatcagcg
    19681 gaatacgggt gatgtcgtca tacatcgccg ctcctttact gatcagctta tgtgcgccca
    19741 tcatttcacc gtgatcggag gtataaatga cccacgtatt ttcacgttgc tctggcgtta
    19801 aggcattgat gacacgtccg atttggtcat caacaaagtc attgcaggca aaatagagcg
    19861 gatggtgata aagcccgtca tcaccgactg gcgatagcat cgcctgcgcc cataagcggt
    19921 gatgttccgg tttgttcgcc aggtcatcct gtgatttctc gccaagttcg tagtaaaaat
    19981 cagcgtattt ctctaaatac tccaccggac aggtgaacgg gtgattcggc tcatcatacg
    20041 aaaccaccat caggaagggt tcctcggcgc gcacgggctg ttgcagaaaa tctaccgctc
    20101 gattgctgat gcggtgcgcc caggtgaagg tttcgtcaat atggttcgcc tgtaaatctt
    20161 cgacgctgtt taggccattg cgccacaggc tgatctcttt ttccgtcagt tcgctaagat
    20221 agttcgcccc atcaaaccag taatcagcat cccactccgg cggacactcg ccagtgccga
    20281 aatagtcatg accgtcgaga tgccatttgc cgatgtaaca ggtgtgatag ccggcatcct
    20341 taaagtagcg ccccatagtg gagatgtttt tgcctggcgc gacgttgttg gtccacgggc
    20401 cggactggtt agcgtagata ccggtgaata gtccggcgcg tgcaggcgta caaaccggtg
    20461 aacaagtgta ggcggaatta aagcgaatac cttccgccgc cagactatca atattttgcg
    20521 tattcagcgg tttaccgcta tagcaaccga ccatattggt ggcctgggta tcggtcaatt
    20581 ggggcgtttc ataggctatc cttcgcgtcg ggagtaaagg ttacggctgg gtcgtgacga
    20641 tgccgccagc tgtcaaaaat caggtagaca atcactacgg cagcgatgaa ataactgatc
    20701 atcgctaacc agcgcgtacc gtagccgccg aattcagcca acccgacata gacgccaatc
    20761 atcgcgaaca ggataccaat tgacgcgatc ttgacgtttt tccacggttt catgtccacc
    20821 gcaaacgcat ctttgaaggt gaacggcgtg gcgcgcgctt tgataaaacc gatcaccagc
    20881 atcacgacca cgttgatgca gaacgtacag gccagcacat agaggaaatg gaagtcgaac
    20941 ttcaccagat agttgatggt gatgtagctg attatgccaa tccccatcgc cacttttgcc
    21001 gccagcgccg ggatgcgcgg gaagaaaaag cccataatga tgatggtaac cagcggcacg
    21061 ttgtaaatgc cgttgagctg tttcatccag ctatacagcc cctgcggcgc gttggcgatc
    21121 cacggcgcta ccaaaaccga aacgatagcg ataaagaaac caaatttgcg cccgacggtg
    21181 accagctgct gcggctcggc attctggtta atgatgcgac ggtaaatacc catactgaat
    21241 aatgtactgg cgctattcag aaagccgttg aaggtactga tcaccgcacc aaataacacc
    21301 gcgccgaaga aacccaccag tggcactggc agaacgttat tgaccagcgt cgggtaggcc
    21361 atgtctgctt tcggtagatc ctgatacaga tgaaacgcaa tcaaccctgg cagcaccagt
    21421 accagcgggt cgagcatttt cagcaccgcc gttaacagcg cccccttttg cccttccgcc
    21481 aggctttttg acgccagcgt gcgttgcacg atgccctgat ttgtacacca gtaaaaggtg
    21541 ttcaccagaa ttaaaccggt aaatgccgcg ccaatcggca agggatcggt cgggccaccg
    21601 actgagttta atttctcggc gtgaacggtg gtaatttgct caatgccctg cataaagctg
    21661 cccttgccca tcgcgatcag gccaaatacc ggcaccatca acccgccgat aaccagccca
    21721 ataccgttga tggagtccgc cactgccatt gcgcgcagtc cgccgatcac cgcatacaaa
    21781 attcccgcca gaccaagcaa aattaccagt agccagatag ccgcaccgtg agaaatctgt
    21841 agcgattccc cgacgtgaaa caggctgttg agcgccaacg cgccggagta gagaacaatc
    21901 ggcagaaagc agacgccggt ggcaatgagg aagcagaagt cgatgataat acgcgtcgtt
    21961 ttatcataac gttcctccag aaaatcgggg atggtggcaa tgccgcgctt cagatagcgc
    22021 ggtaaaaaga ttagcgcgag gaagatcagc gtcaccgctg aagtcacttc ccagcccatc
    22081 accgacatgc cgcttttgta ggcctgcccg gaaagaccaa ccagttgttc cgtggaaagg
    22141 ttggttaaca ttaacgaagc ggcaataacc ggcgctttta gtgaacgtcc ggcaagaaaa
    22201 tagccttgtt gcgatccggt atctgttttg cgaaccttcc actaggtgat caccgccacc
    22261 agcagcgtaa aaccgacaaa actcaagatt tgtaacgaat tcatctcata gcccttattt
    22321 atgtttatgt cccggacgca tgaatatagg gagaggtaaa atgtgtcggg tcaggcagtc
    22381 agattcttaa aatacaaacg tcgtatccct gaacggattg ttttctgtta agttcaggtt
    22441 gtgagcataa ttcgccggag ggatattaaa atgaatggaa aattgcaaag ttcagatgta
    22501 aaaaacgaaa ctccgtacaa tattccctta ttgattaacg aaaatgtgat ctccagcgga
    22561 atttctctga tctcgctctg gcatacctac gccgacgagc attaccgggt gatctggccg
    22621 cgggacaaga agaaaccgct tatcgccaac tcatgggttg cggtgtatac cgtacaagga
    22681 tgtgggaaaa ttcttttaaa gaatggcgaa caaataactc tgcatggcaa ctgtattata
    22741 tttttaaagc caatggatat tcactcttat cactgtgaag gtttagtctt ggaacagtac
    22801 tggatggaat ttacccccac cagtatgatg gatattcccg ttggtcagca aagcgttatt
    22861 tataatggcg aaatttataa tcaggaactc accgaagttg ctgagttaat aacttcacca
    22921 gaagcaataa aaaataatct ggcagtcgct tttctgacga aaattattta tcagtggatt
    22981 tgtcttatgt ccgcagatgg taaaaaagat ccacaacggc ggcaaattga aaaattaatt
    23041 gccactttac atgccagtct gcaacaacgc tggagcgtag ctgatatggc tgccacgatc
    23101 ccctgtagcg aagcctggtt gcgtcgtctg tttttacgct ataccggcaa gacgccgaaa
    23161 gaatattacc tcgatgcgcg tctggatctg gcgctatcgc tattaaagca acaaggaaac
    23221 tcggttggcg aagtcgctga tacgctcaac ttcttcgact cctttcattt cagcaaagcc
    23281 tttaaacata aatttggtta tgcgccgtca gccgtgctaa agaatacgga ccagcaccca
    23341 acggatgcca gtccacacaa ttaacgtaac tctggccagt acgctttgtt ggcctccagc
    23401 aactcatcca gaatagcttt ggcgaccgag gcgctcggta ccgttttcga caacgtaatc
    23461 gcctgccaca ggtgctgata tgaacgctgt tcccaggcgt ccacgaccag tttttccacc
    23521 gccacttgct gactcattaa ccctttctga aactgcggga tatcgccgac caccagtggt
    23581 tcaggccgta gtagcgcacg cgcatctgtt tgcgatcttg cagcccaaca atttgcgcca
    23641 tccggctttc aataccgatt ggcatgtcac agatgttgag gattttcgca ttcgggcgca
    23701 gacgacgcgt ggcttctgcg acaattgctg ccgggttgga gtagttgagc atctaggcgt
    23761 ttggtgaata tttttccata taatccacca gttccaggac gccgccaatg gaacgcatgc
    23821 cgtacgctat tccgccgggt ccgcaagttt cctgaccaac aacaccgtgg cgcagtggga
    23881 ttttttcatc cagttcgcgc atcgggtact tgccgacgcg gatgtgcgcc atgacaaaat
    23941 caacgtcgct gaacgccact tcaggatcgg tggtgtaact aaacgcaatg tccggcgctt
    24001 tttctttaag gatgacttta caggcttcgg caatcacttc ctgccgcgcg ccatcgttat
    24061 cataaaattt cagtgcacga agcgggaaac ggtcctgatt cgccaggagc atcaacacga
    24121 tgcctggcgt aaaggtgctt ccaccgcctg cgacaaccac tgagaatttg gtcattatac
    24181 tgcctccgta atggcaacat tttctgctga ttgatgagaa ttaattaagc tatcgagctg
    24241 ttcacgcaat tgggatacat gcagaccaat gatcacctga atggcatcgc cactacggaa
    24301 gacgccgtgc gctcccagct ttttaaagac ttcgtcatcc agcgtttgtg acatgtcatg
    24361 cagtgcaata cgtagacgcg tcgcgcaatt gttaatgctg gagatattgc cgaccccgcc
    24421 cagggcttgc aggataccgg cagcctgatc cagctctttt tttggctctg cagcggtggt
    24481 ttggcctcgc gaggctttgt attcggcttt tgagtagagt ttcacttccg catcttcacg
    24541 tcccggcgtg cacatgttga actgcagaat cagggtgcgg aaaaccacga agtacagcag
    24601 ggtaaagcac aacccaatgg cgatttgggt cagcatcata tccgcgtggt tgctgaacat
    24661 cgggatccag ttttgcggta aaacctggtc aatcagacct ccgcccatgt tgcccaccac
    24721 accaaagaga tacattacgg tcgacattga ggccgccagc acggcgtgta ccgcaaacag
    24781 caacggtgaa atgaacagga aggtaaattc cagcggttcg gtaattccca ccagcatggc
    24841 ggttaaggtt gcgggaatca gcaagcccgc cacttttacc cgattttccg gtgcggcagt
    24901 gaagtacatc gctaaagaaa tgcccacggc accaaagatt tttgagttac cgtgcagggc
    24961 aaaaccgcct tccgggaaca acgatttcag cggctcggcg ctcagactga actcttgcag
    25021 atgctgcgcc cagtacatct gaatgccgcc ttcaacagct gccggaccaa agataaacgg
    25081 tccgtagatg aagtggtgta aaccggttgg gatcagaata cgttcgagga aggtgtaaac
    25141 ccacacccca agcgcacccg ccgaacgcag gaacgcttgc agagattcaa tccccatttg
    25201 tacttttggc cagccgagca atgtcagcca ggcacagggg atcatcacca agaaggcgat
    25261 aatcaccaca taagacgtcc cctggaaaat gccgagaaaa accggcagtt ttttatcgaa
    25321 cagacggtta tgcagcgccg tcacaatgcc ggaaatgata attgcgccga taatgctggt
    25381 atcgagggtt ttaatcccgg ccatcattgt cagaccacta cctgccaccg cgtcctgagt
    25441 gaaatcgacg ccgaagtagc ttccccaggt cattcccatc gcattgatga aatagttcca
    25501 ggtcaggaaa ctcaccatca ccgccagaca agcacgtccc tgcgcttgct tagcaaggcc
    25561 aatgggtaaa ccgacagcaa aaatcagcgg catattacgg aataccgtcc aaccgccctc
    25621 ttcaataatg tgtacgattt gcgcgaataa actgttcgga tcggtcagtg attccccgac
    25681 aaacatcggg ttttgcagca agatggcaag acccaccaca atcccggcga agggaaacag
    25741 cagcactggc gtgaacatcg cgccgccaaa gcgttgaatt tgactgagca tttccacatc
    25801 ctcttttctc aattctgtaa gtaggaagcc tgattggtta tgcggcagac ctggagcgag
    25861 gataggaaga tgcgatcggc agaacatgac gacccaatgg gattcgtgat cgctttcatg
    25921 cttttcgttg ggcggtagag atctacgttt tcgcgaaaaa gtagacatct gtgagcacag
    25981 gtacacagca tcgaacacat ctttaaaaaa aagatttttt tcaatcgatt aagcagaact
    26041 tgtgggcgca ttacccgtgc ttgcaggcaa aaaagagagc tagagatgat ctacaaaagc
    26101 attgcggagc ggttaagaat tcgacttaat tccgcggatt tcacgctaaa cggccttctt
    26161 cccggtgaaa aaaagctggc ggaagagttt gcggtatcgc ggatgactat ccgtaaagcc
    26221 attgacctgc tggtagcgtg ggggctggtg gtccgccgcc acggcagcgg cacttacctg
    26281 gtgcgcaaag atgtgctgca tcaaaccgcc agcctgaccg gactggtgga ggtgttaaaa
    26341 cggcagggaa aaacggtcac cagccaggtg ctgatttttg aaatcatgcc tgcgcctccg
    26401 gccattgcca gccagttacg gattcaaatc aacgagcaga tctacttctc ccgtcgcgtt
    26461 cgttttgtgg aagggaaacc gctaatgctg gaagacagct atatgccggt aaaactgttc
    26521 cgtaaccttt cactgcaaca tctggaaggg tcgaagtttg aatatattga acaagagtgc
    26581 gggattttga ttggcggtaa ttatgaaagc ctgatgccgg tgctcgccga tagactgctg
    26641 gcgcggcaaa tgaaggtagc ggaacacacg ccactgctgc ggatcacctc gttgtcatat
    26701 agcgagagcg gggagttttt gaattattca gtgatgttca gaaatgccag cgaatactag
    26761 gtggagtacc atttacggcg cccccacccg gaaaagagtt aaccgatact ccagaagagc
    26821 accgccagta attggggggt gataatgcgc aggaacatca ctaacggata gacagtggcg
    26881 taagagagcg ccgccgcacc actggttgga tgaagattat tagcaaacgc cagcgccgga
    26941 ggatcggtca tggaacctgc cagcatcccg cacatggtca ggtaattcat tttggctaac
    27001 atccgcgcca gaatgccaac agtaatcagc ggaacggcgg tgatcagggc accataacca
    27061 atccagctta gcccttcgcc attgaccagg gtattcacaa aatccccacc agatttcaga
    27121 ccaacgaccg agaggaacag cacgatcccc agctcccgca gcgcgaggtt ggcacttggc
    27181 ggcataaacc agtacagctt gccgatactg ccgatacgcc cgaggatcaa cgccataatc
    27241 agcggaccgc ccgccagccc cagtttcaac gcggccggga atcctggcac aaagacggga
    27301 atagaaccta acaatacgcc aagcccgatg ccaataaaca ctggcagcat ctgaacctgt
    27361 tgcagttttt gctgcgcatt ccccagcaca ttggcaacgg catcaattgc ggacggacgc
    27421 cccaccaggt tgaggatatc gccgaactgc aggctgatat cgccactggc gaccagttcg
    27481 accccggcac ggttcaggcg cgagataaca acgtcatagc gttctttaaa gtgcaggtcg
    27541 cgaatacgtt ttccgagcac gttttcattg gtgaccacca cacgctcgac gcgcaaatca
    27601 gtgcctttcg tagacagcga ggtatcgacc tcctgaccaa tcaccagttg cgcattatgt
    27661 aaatccgctg gctgccccac cagatgcagc aaatcgccca gttggataat ggtatctggc
    27721 gaaggtactt ttagggtttc ttctcgtttc agacgcgagc agataatttt gtcgccgttg
    27781 agaatcggta catctttaat ggctaaatca tgcaggttag ggttctcaac gcgaatattg
    27841 atagtcttga tcagcgcgcc gccattggtg cgtgaagact cgtgctgctg agcttctgtc
    27901 tcgacattga cgcggaaaat aacccgcaac atccacatgg tgaacaaaat cccgcaaatg
    27961 ccgaatggat acgccatcgc gtaactcatc cccatctgat cgaccatttc cattggtgta
    28021 cccaggtcgc gcaagatctg ctgccctgcc cccagcgctg gcgtattggt taccgcaccg
    28081 gagaaaatcc ccagcactac cggcagtgga atatcaaaca gtttatgcag gatggcggta
    28141 accagaccac cgatgatgac gatcagaaca gcaaacaggt tgaggcgtaa tccggagacg
    28201 cgcaatgagg caaagaagcc cggccctacc tgaatcccga tggtataaac gaacaggatc
    28261 aggccaaatt cctgaataac atgcagcata tcgctactta atgtcatccc cgcctgagaa
    28321 acaaaatggc cgacgatgat cccaccaaac agcacgccgc caatacctaa tcctatgccg
    28381 cgaaatttga cgttgccgat aaacaaaccg acgactgcca ccaaagccag aatactgacc
    28441 gttaatgcta tatcactcat cgtccatttc tcttgagata acattttaat tgctacggat
    28501 tatggctgaa tcggtgaagc agatttgcct tatagcgcac attatcacgg gaaagaacgt
    28561 gccgaaatat cttaaacagt cgaaatttaa cgcattgttg aagaagaaag acaaaaaaag
    28621 ccccaccaga atggcggggc aaagagaata gctagttagc tatttaacgc gggacgttcg
    28681 ctgatagcga tacgctgcgc tgcgatgggt tcaggctcat tacgaattaa atcaatatgc
    28741 agtaaaccgt ttacgaaggt tgcgccagag acttccatat tttcagccag cgtaaaactc
    28801 aggctaaatg gctgattcat aagcccttga tgcagccatt ttttctcttc ttttggctgc
    28861 tccggcgtgc cttttacgct caggcgcgta ccttccagtt gaatctctaa atcttcctga
    28921 cggaaacctg ccagcgcaag ggtaatgcgg tagtggttat cgtcgctttt ctcaatgttg
    28981 tacggcggga agctctggct ttcaccggcg ttttgcagtg cgttggccag tttgtcaaaa
    29041 ccgatccatt gacgcatcag tggggataaa tcgaagttac gcatagtcat ttctccttct
    29101 aagaagcgag taagtacctg caaatccgaa gattcgcata tgctccctga cggcgagcat
    29161 ggagatgtca ggccgcgcca ggcggcctta gggaattagt tgatttcgat acggcgcggt
    29221 tttttcgctt ccggaatcac gcgttcgaga tcgatataca gcaaaccatt taccaggtta
    29281 gccccacgaa catgaatgtt ctcagctaac tggaatttgc gttcaaagtt gcgttcagcg
    29341 atgccctggt acagataggt gcgctctttt tgttcgtcgg cgtgagcacc tttcaccacc
    29401 agcagattat cctgggcggt aatttccagt tcgctctcag caaaaccagc cacagcgata
    29461 gcaatgcggt aatggttttc gtctaccagt tcaacgttat acggagggta gccgccatta
    29521 ctctggctct ggttgttttc taagtggtta aacaaacggt caaatccaat agcagaacgg
    29581 taaagcgggg ataaatcaaa gttacgcata atcaatagct cctgaaatca gcgagaatgt
    29641 aagaccttcc acaatggaca ggtcaggtag ccagaacacc catcaggcgc gtcctcatcg
    29701 actacgatgt aaaatgggtc tggaaatgaa cttttcaagc cttatcagcg gacttttttt
    29761 tgcagtttat ggtctattgc atagactgag ggggcagcac gaaacgttaa agtgcgatag
    29821 ccgccacagt gcgacgtaat gatgatgtta ttttcagcaa gggatcgcta taactcatca
    29881 ggccaacacc cacgatcaac agatgaattt attatgataa gaaatgtgtt gttagcgttc
    29941 atgatatgca gcggaatgac attactcgga ggatgctcca gcgtgatgtc ccataccggg
    30001 gggaaagaag gaacgtatcc cggcacccgc gccagcgcca ccatgattgg cgacgatgaa
    30061 accaactggg gcactaagtc gctggcgatt ctcgatatgc cgtttaccgc tgtgatggac
    30121 acgcttttac tgccgtggga tgtgttccgc aaagacagct cggtgcgctc gcgcgttgaa
    30181 aaaagcgaag ccaacgcgca agccaccaat gcggttattc caccggccag aatgcctgat
    30241 aactgattta gcgatcggtt tcggtgatcc acagctggcc accttccatc cacgccagcc
    30301 attgaccatc cggcgagaag accacggcgt ccgcagaagg tggattggcg tggttttcgg
    30361 ttaaatactc aacctcgccg ctttgcgcat gggcacaaac aattcgatta tccagcacaa
    30421 agcccaacca ttctcctgac ggatgccagt taaatgcaga ctgaatatcc gttttgttat
    30481 gggttaactt gcgcggttcg ccgccctgtg gcgagataag ccacagttgc acaatgccgt
    30541 tatcatcacg cattaaaaac gcgatttgcg taccctgcgg attacagcgc acccagtggc
    30601 gcgggtaggg ttgtttccgt tccacttaac ggcgcatcac ctgccgattt ccagctagct
    30661 tcatcttgcg gtaactcaac gataaatagc tccggcactt tctcgccctt tggcgaaagt
    30721 gtgtcgccaa taaacgccag cgcgtgattt cctacccatc cttcttcata agcacgattg
    30781 atttcatcac tgccaggctg cggcgtgggc gtggttttac tcaccagcac acaccagtgg
    30841 ctaccgctgt attcacgcgg atgttgcttt tgtacgttga ccgggccaaa cggtgcagca
    30901 accccgacgt ttcgcaaatc cagcgccgga tagagttgat gcattacatg gtcgttatag
    30961 gtaaagctcc ccctttcacc gttcgggcta aagacatgca catggctgcc accgcgcagc
    31021 gctcctggcg tatacggcgc ggtaatatcc attgcatcga gattgctcac cttgccgcct
    31081 tcagcaatga ctccgcgtcg atgatggaag tcgtaatgcc atgtttcatc aggattttca
    31141 gggccgtgaa taaagacata tttctctgac ttcggatgaa cggtcaccac gccgacgtgt
    31201 gcgccctgtg acgcgcgata gataacctcg acctcgcctg tatggatatt cacacgctca
    31261 atggtctcgc cggtaaacga cgccccagaa ggacgcacgt caaataccag ccactggctg
    31321 tcgggcgtcc aggtattggt attggtgagc aggtgattac ggggagcaaa ggttatctgt
    31381 ttcatggcgc ggtcctgata caaaacggtt cccgccatca tacttcacaa ggacttcact
    31441 ttcagacaat agtcttttgc catcgtgatg aatgtgagtg aaaagatgga acatttcgac
    31501 gtggcgatta ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt agccggcaaa
    31561 atgcaggtga tcgcgctgga taaaaagcac cagtgcggta ctgaaggttt cagcaaacct
    31621 tgcagatttt cagcgtcaaa actgtcgacg tcgccgcatc gctcacgcgt aactaccagc
    31681 gaagctatat caatattaat cgccatgctt tcgacttgtg gatgaaatcg ctgatccccg
    31741 ccagcgttga ggtttatcac gatagcctgt gccgaaaaat ctggcgtgag gatgataaat
    31801 ggcatgtcat ttttcgtgca gacggctggg agcaacatat taccgcccgc tatctggtcg
    31861 gtgccgatgg cgcaaactcg atggtgcggc gacatctcta cccggatcat caaatccgta
    31921 aatatgtcgc tatccagcag tggttcgcag agaaacatcc ggtgccgttc tactcctgca
    31981 tctttgataa tgcgataact gactgttact catggagtat cagcaaagat ggttatttta
    32041 tctttggcgg tgcctatcca atgaaagacg gtcagacgcg tttcacgacg ctgaaagaga
    32101 aaatgagcgc ctttcagttc cagtttggca aagcggtgaa aagtgagaaa tgcacggtgt
    32161 tgtttccctc acgctggcag gattttgtct gtggtaagga caacgccttt ctgattggtg
    32221 aagcggcggg atttatcagc gccagctcgc tggaggggat tagctatgcg ctggatagcg
    32281 cagagattct gcgtgcggtg ttcctgaaac agccggagaa gagcaacgcc gcttactggc
    32341 gcgccacacg caaactgcgt ttaaaactct tcggcaagat agtaaaaagc cgatgcctga
    32401 ccgcaccggc tttaagaaag tggattatgc gcagtggtgt ggcgcatatt ccacagttga
    32461 aagattatcc aacgcgcttc acatcaccca ccagcaggat gtaagagagc gcgccaatca
    32521 acgcgacggc ggagatataa accagtgcag gcgcgaaacc gtaaccctgc gccaggtacc
    32581 ccaccaccag cggaacggtg atgccgccca gaccaccggc gaagttaaac acgccaccgg
    32641 ttaaaccaat caggcgcatc ggtgccagag aagagaccag cgaccaggta atcgaagcaa
    32701 aaccgttgcc gaagaatgcc agcgccatca ggcacataat catcatcgga tcgttggtgt
    32761 agttagcgcc cataatgcag gtggagatca gcaagccgca gataatcggc gttttacgcg
    32821 caaagcccag tgaaaagccc ttacgtacca gcagatccgc gacccagcca gagagcagga
    32881 cgccgacaaa cgccgcgagg aatggcaccg tggtcataaa gcctgctttc agcgccgtga
    32941 ttcctttttc ctgggttaaa tagttcggga accaggttaa gaaaaaccac agtgcagaag
    33001 ccaccgcaaa ttgcccaaga taaacgccga tcagtttacg atggaacacc agtttccagt
    33061 ctttggctgt taacggctga cgcgcctctt tcttcaccgg cgcatcacca tccaccagac
    33121 cgccgccatc acgaatgtaa tccagttcag ctttgctgat acctttggtc aggcgcggcg
    33181 gctgataaac cttaaaccaa atcagcgacc agataatgcc gataccacca gtgacaatga
    33241 acacccagtg ccagctcaac atctcctgaa tccagatcag cagcggagtc aaaaacgcca
    33301 gaccgacaaa ctgaccagac gtataaaaac caacggcgga agcgcgttca tgttccggaa
    33361 accagctggt caccatccgg ttattggtcg ggaaagcggg cgcttcgaaa ataccggtta
    33421 tcgcacgcag accaattaat gacattaatc ccgtggcaaa gccctggaat aaagtcgcca
    33481 ctgaccaacc aaatatcgca ataaaataag tcacgcgaga acctacgcga tctaaaaacc
    33541 aaccgccggg gatctggcat agcgtataaa gccaggcgaa ggccgaaaat acatagccca
    33601 tttccgcttt cgtaatgccg aactcttcct gaatatgggc ggaagccacg gccaggttag
    33661 cgcggtcaac ataacaaatg accaccgtaa taaagatcat caccagcgtc agataacgcc
    33721 gacgccccgg ctttgctgca ttaacgggaa tatccatagc gagctttctc cagattttgg
    33781 gcatagcgaa gccgctcacc atgccctgta atttacagag ggttatattt ttgtattgct
    33841 gttttagtgc ccgatgaggg gcttacgtgg caggaattac cactctgcta cgctgttatc
    33901 ttcatgacgc cagagcggat tacgccagtc cggggcattt ttactgaact caataacttt
    33961 agcttcgtcg atttccacac ctaagcccgg tttcgttaac ggtttaaaga agccgccaac
    34021 catgctgaag tcttctttgt ttttcacaaa gtcgagtaac tccgcgcctt tgttgtaatg
    34081 aatgcccata ctttgttact gaagtaccgc gttataggaa acaaagtcga tatgcaggca
    34141 agccgccagt gcaatcggtc cgaacggaca gtgcggcgca agggtcacat cataggcttc
    34201 tgccattcca gcaattttgt agcattcggt aataccgcct gcatgggaga gatccggttg
    34261 cagaatcgaa ataccgcctg cctccagcac gcgtttaaaa tcgaaacgtg agaacatgcg
    34321 ctcacccgcc gccagtggaa tatgcgtttg tgccgccaat ttcgggtagt attcggcttg
    34381 ttccgccagc accggctctt caataaacag cgggcgatac ggctccagtt ctttaatcag
    34441 cactttcgcc attggcgcgc tgacgcgacc gtggaaatca agaccaaact caatctgatt
    34501 gccaaaagct tcacgaattt gtgccacggt gttgactgcc gcatctaccg cgcgggagtt
    34561 atcaattagc cccagttctt cacaaccgtt cagtttgaag gtatcgaagc cgatttcgcg
    34621 cagggtttta atgccgtcga taacatccgc cggacgatcg ccgccgaccc aactgtaggc
    34681 tttaattttg tcgcgaacca ggccgcccat cagttgccag actggcgcat tcagcacttt
    34741 gcctttgata tcccataacg cctggtcgat cccggcgatg gcgctcatca ggattggacc
    34801 gccacgataa aatccggcgc gatacatcac ttgccataag tcgttgatgc gcgaaggatc
    34861 ctgaccaatc aaatagtcac ccagctcgtg aactgccgct tccaccgtac gggcgcggcc
    34921 ttcaatcacg ggctcgcccc aaccgaccac gccttcatcg gtttcaattt tcaggaacat
    34981 ccagcgggga ggtaaacgat acgtggtaat tttggtgatt ttcattgcac tgcctctcga
    35041 tacgccttaa caaatgctgc tgcctgctgc gcggtgcgtt ctacagactg cccggcgcga
    35101 taaagatcgc tgcctaagcc cgccccaaca caacctgcgt ctatccactg cgccaggttt
    35161 tctggcgtca cgccgccaac ggcaaagact gcgatgtccg gtggtaatac cgcttttaac
    35221 gctttgatgt attgcggacc aaaagccgat gacggaaata ttttcagcgc ctgcgcgccc
    35281 gcttcgagcg cggtaaaggc ttcggtcgcc atcgcgcagc cggggcaaac ggtcatgccg
    35341 tagcccaccg cgcggcggat cacttcacta tggatattgg gcgtaacgat gagctgacag
    35401 cccatcctgg cgagcgcatc gacctgttca ggtttcagta ccgtacctgc gccaatcaac
    35461 gccttgtcgc catacgcatc aacgatggcg ggaatgctct gctcccattg tggggaattc
    35521 agcgggattt caaccgcgtc gaacccggcg tcaatcaccg cgccaacatg cgccagcgcc
    35581 tcgtcgggcg taatgccgcg caaaatggcg atcagcggga gtttagtttg ccactgcatg
    35641 agcgatgctc cttataccag cctgaaatgc cgtgtcgccc gccaccgccg ccacgtcgca
    35701 acccatcgcc tgaaaggctt gctggtagcg cgcggtcagc gatgttccgg cgacaagggt
    35761 gatggcgtgt tgatgggtca catagtcgcg catactggca acctctgcgc caatcaacaa
    35821 accagagaga atgttcgcgg agaagtgttc ccagcacatg cgaggcgcga acttcaaaaa
    35881 gctgcggcaa tatggcgggc gcattaaggc cgcgctcaag gccagccgcg aaggcatcgg
    35941 cagagttttc ctgcggcggc aaacctgcgc caatcaatga gtgatttaac agtaaatgat
    36001 gtaattcacc ggtcatcacg gtgcgaaaat cgttgatttg ctggctatcg gcctgcaccc
    36061 atttgcaatg cgtaccgggc ataacataaa gagaggaagg agccagagct cgcgcgccga
    36121 tcaattgtgt ttcttcgccg cgcatcacat tgtggttatc gtcatgagag acgcataatc
    36181 cgggaataat ccagatattg tcgccaacag acgttaattg ttcgccaata gacgaaaaac
    36241 gggcaggaac agataaatac ggagcaactt tccagccgac attgctgccg accattcctg
    36301 ccatcaccac tggcgtattt tcttcacgcc agtcggtcgt gacttctgct aacaccgcag
    36361 ccggagattt tccgttcagg cgcgtgacgc ctgcttctga ttgcctgctc tccaggcagt
    36421 ggtcgccctg ataaagccag gcgcgcagat tggtcgatcc ccagtcaatt gcgatgtagc
    36481 gagctgtcat gtgatttcct ttaaccttcg tgtcgagctg gcaatcatgg taagcgccgc
    36541 ctgctctgcc gcatcgccgt cctgatgccg tatcgcatcg aacagcgcct tatgttcctg
    36601 gagcgtttgc ggcatgttgg cctcatcgcc catccaggtt cgttcaaaaa ccgcccgctg
    36661 cagcgaactg atcgcaatgc taagttgctg taacaccggg ttatgcaccg actgcagcac
    36721 cgcctcgtgg tagcgaatat ccgcttcgtt aaacgcttcg cggtcctgat tgttggcaat
    36781 catctcgttc agcgccgatt caatctgcgc cagatcgctg gaggtcgcgc gctctgctgc
    36841 ccaacgggca atcgccggtg ccaccagatt tcgcacttcg ctcatggcac tgataagccg
    36901 tgggtcgtag tcgttttcca gcacccattg cagtacgtca gtgtcgaggt aattccactg
    36961 gttacgcggt gccacaaacg ccccgcgata acgtttcatt tcaatcagcc gcttcgccat
    37021 cagcgaacgg aacacctcac ggatgatgtt gcgcgaggtt gcaaactctt cacagagctc
    37081 cgcctcagcc ggaagtggcg agccaggtac gtatttgccg tgaacgatct gtttacccag
    37141 cgtaatgaca atgcgatcgg ttttattgag agtcatggag agtccttgtg ctctgtatgt
    37201 tcttctctac tttaccccga tcgatgcata acgcggcaac tttgtagtac cagcgtgatg
    37261 acgttcgcgt ttgccgtgcg tgtaatgtag tacaaactta tattgttgtg ctacaattta
    37321 gatcaaaaaa agaacaatgc ataaaaaaat gacatgcgtc gggcagaaat ctgaaaaggg
    37381 atatcaggcg ctaaacagga gggaaagaag agtatgcttt caacggctta gctactcgtt
    37441 taaaagatta atcatgaagt tgaattttaa gggatttttt aaggctgccg gtttattccc
    37501 actggcgctg atgctttcag gctgtatctc gtatgctctg gtttcccata ccgcaaaggg
    37561 tagttcagga aagtatcaat cgcagtcaga caccatcact gggctatcgc aggcaaaaga
    37621 tagtaatgga acaaaaggct atgtttttgt aggggaatcg ctggattacc ttatcactga
    37681 tggtgccgat gacatcgtta agatgctcaa tgatccagca cttaaccggc acaatattca
    37741 ggttgccgat gacgcaagat ttgttttaaa tgcggggaaa aagaaattta ccggcacaat
    37801 atcgctttac tactactgga ataacgaaga agaaaaggca ctggcaacgc attatggttt
    37861 tgcctgtggt gttcaacact gtaccaggtc actggaaaac ctaaaaggca caatccatga
    37921 gaaaaataaa aacatggatt actcaaaggt gatggcgttc taccatccat ttaaagtgcg
    37981 attttatgaa tactattcac ccagaggcat tccggatggt gtttccgcag cattactgcc
    38041 agtgactgtt acgctggaca tcattactgc accgctgcaa tttctggttg tatatgcagt
    38101 aaaccaataa tcagtaagcg ggcaaacgcg tttatgctgt ttgcccgccc acagattaat
    38161 tcagcacata cttctcaata gcaaacgcca cgccatcttc aaggttggat tttgtgacaa
    38221 agttcgccat ttctttcacc gaaggaatag cgttacccat cgccacaccg acgcctgcat
    38281 attcaatcat tgcgatgtcg ttttcctgat cgccaatcgc catgatttct tccggtttaa
    38341 tacctaacac gtcggccagt gatttcacac ccgtaccttt gttaacgcgt ttatcgagga
    38401 tttcgaggaa gtacggcgca cttttcagca cggtatattt ctctttcact tcctgcggaa
    38461 tacgcgcgat agcctggtcg aggatggcgg gttcatcaat catcatcact ttcaggaact
    38521 gggtattggg gtccattttc tccgcttcgc agaacaccag cggaattgtg gcaacgaagg
    38581 attcatgcac cgtgtagtag ctgatatcgc ggttagcggt gtacagcgtg gtgcggtcca
    38641 gggcgtggaa atgagaaccg acttcgcgag agagtttttc caggaaacga tagtcgtcat
    38701 agctgagagc ggtttgcgcc acggtgctac catcagcggc cttctgtacc agcgcgccgt
    38761 tataagtaat gcagtagtcg cccggctgtt ccatatgcag ctctttcagg tagttgtgca
    38821 cacctgcata cggacgaccc gtcgttagca cgacattcac gccacgggcg cgagctgcgg
    38881 caatcgcatt tttaacggcg ggtgaaatgg tgtgatcggg cagcagaagg gtgccatcca
    38941 tatcgatagc aatgagtttg atagccatga gttcccaagg tagattagtt cctgacccat
    39001 gctaacgcga ttccgctcaa aaatcagtac aacacccgag ggaaaagggg atgcaacgcg
    39061 ggttgcatgc tccccatttg cttagcggaa gagtttccct ttcagcagtt ccatgcctgc
    39121 ggaaagcaga tcgttattgg cttgtgctga cacttcacct tgcggtgaga gcgcatcaat
    39181 aatcttcggc aattgctctg ccagtaaaca ggaagccgta ctggtatcca cgccaagttt
    39241 ttgcccgaga tcggacaccg catttgtgcc gagcgccgat tccagttgct cgccgctaac
    39301 tgattgattt ctctgttgat tactcaacca agttgagaga atggccccta agccgccact
    39361 ttgcagtttt tccagcagca cctgaatgcc accctgctcc tcaacccaac ttaaaatcgc
    39421 ctgatatttc cccgcatcgc ctttcagaaa ggcaccgaca acttcatcaa aaagccccat
    39481 gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt atagatctgc gggatgataa
    39541 ttgcggattg cagaaataaa aagggcggag atgatctccg cccttttctt atagcttctt
    39601 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc atgcaaaatc aatatattgc
    39661 aggagtctcg taggactaat aagcgtagcg catcaggcac gctcgcatgg ttaacgccat
    39721 taaatatcaa tattcgccgc tttcagggcg ttctcttcaa taaacgcacg acgcggttct
    39781 acggcgtcgc ccatcagcgt ggtgaacaac tggtcagcgg caatcgcatc cttaacggta
    39841 acgcgcagca tacgacggct ttccgggtcc atggtggttt cccacagctg ttccgggttc
    39901 atctcgccca gacctttata acgctggatg gagaggccac gacgggactc tttcaccagc
    39961 cagtccagcg cctgctcgaa gctggctacc ggctgacgac gctcgccacg ttcgataaac
    40021 gcatcttctt ccagcaagcc acgcagtttc tcacccagcg tgcagatacg acgatattcg
    40081 ccaccggtga taaactcgtg atccagcgga tagtcagtat ccacaccgtg ggtacgcacg
    40141 cgaacaatcg gctcgaacag gttttgttcg gcattggtgt ggacatcaaa cttccactgg
    40201 ctgccgtgct gttctttgtc gttcagttcg ctgaccagcg cgttcaccca gcgggtaacg
    40261 gtctgctcat cagaaaggtc agcttccgtc aacgtcggct gatagataag ctctttcagc
    40321 attgctttcg gataacgacg ctccatgcgg ttgatcattt tctgcgtcgc gttgtactca
    40381 gataccagtt tctctaacgc ttcgccagcc aatgccggtg cactggcgtt ggtgtgcagc
    40441 gttgcgccgt ccagcgcgat agagatctgg tactgatcca tcgcttcgtc gtctttaatg
    40501 tactgttcct gcttgccttt cttcactttg tacagcggcg gctgagcgat gtagacgtga
    40561 ccgcgttcaa cgatttccgg catctgacga tagaagaagg tcaacagcag cgtacgaatg
    40621 tgcgagccgt cgacgtccgc atcggtcatg atgatgatgc tgtgataacg cagtttgtcc
    40681 gggttgtact cgtcacgacc gataccacag ccaagcgcgg tgataagcgt cgccacttcc
    40741 tgagaagaga gcatcttatc gaagcgcgct ttctcgacgt taaggatttt acccttcagc
    40801 ggcagaatcg cctggttctt gcggttacgc ccctgcttcg cagagccgcc cgcggagtcc
    40861 ccttccacca ggtacagttc ggaaagcgcc ggatcgcgtt cctggcagtc tgccagtttg
    40921 cccggcaggc cagctaagtc gagcgcacct ttacggcggg tcatttcacg cgcgcgacgc
    40981 gccgcttcac gggcacgggc agcatcgata attttgccaa ccacgatttt cgcatcagtt
    41041 gggttttcca gcaggtattc cgccagcagt tcgttcatct gctgttcaac cgccgatttc
    41101 acctcagaag aaaccagttt gtctttggtc tgggaggaga atttcgggtc cggcactttt
    41161 acggaaacga ccgcaatcag gccttcacgc gcatcgtcac cggtggcgct gactttggct
    41221 tttttgctgt agccttcttt gtccatgtag gcattcaggg tacgggtcat cgccgcacgg
    41281 aagcctgcca ggtgagtacc gccgtcacgc tgcggaatgt tgttggtaaa gcagtagatg
    41341 ttttcctgga agccatcgtt ccactgcagc gccacttcaa cgccaatacc gtctttttcg
    41401 gtggagaagt agaagatatt tgggtggatc ggcgttttgt tcttgttcag atattcaacg
    41461 aacgctttga tgccgccttc atagtggaag tggtcttctt tgccgtcacg cttgtcgcgc
    41521 agacgaatgg aaacgccgga gttgaggaac gacaactcac gcagacgttt cgccagaatt
    41581 tcatattcga actcggtcac attggtgaag gtttcgaggc tgggccagaa acgcaccatg
    41641 gtgccggttt tttcagtctc gccggtaacc gccagcggag cctgcggtac accgtgttcg
    41701 tagatctgac ggtgaatttt accctcgcgc tgaataacca gctccagttt ttgcgacagg
    41761 gcgtttacta ccgaaacacc aacgccgtgc agaccgccgg acactttata ggagttatcg
    41821 tcaaatttac cgcctgcgtg cagaacggtc atgatcactt ccgccgccga tacgccctct
    41881 tccgggtgaa taccggtcgg aatgccgcgc ccgtcatcct gtacagagac agagttatcg
    41941 gcgtgaatgg tgacgataat ttctttacag tgacccgcga gcgcttcgtc gatagcgtta
    42001 tctaccacct cgaataccat gtggtgcaga ccggtgccgt catccgtgtc gccgatatac
    42061 atacccgggc gcttacgcac cgcatccagc cctttcagga ctttgatact ggaggagtca
    42121 taagaattcg acatcaacgt ttctcgctca tttatacttg ggttaatccg ttattttacc
    42181 cttttccacg gtaaacatct tcgaattttc gtccgacatg tctataacgt gttcagcact
    42241 gatcgcgctg acaaagacct gtgattgcgt cgcttttaag cggctggcaa gcaacccgcg
    42301 acgctcatca tcaagctcag aggcaaaatc atctatcagg tagagacacc gccgcccgct
    42361 ttcacgagtg aggaactctc cttgcgccag acgtaaggcg cacatcaaca gcttaagctg
    42421 cccacgcgat aaggtatctt ccaccggcgc accgtcggcg cgaatgcgta agtccgcttt
    42481 atgcgggcca tgcgcggtgt aggttagctg gcgatcgcgt tcaaaattac gttccagcac
    42541 ctcagcgtac tctgtctctt tctcccagcc gcgctggaaa gagaaagtca gagaaaactc
    42601 agggagaaat tgcttacagg tatcatccat atcggccgcg ataccggcgc tatactccgc
    42661 gcgccaggtg ctgatttgct ccgccagcgg gatcagttct ttatcccacg ggcgtagctg
    42721 ttcgtaacga gtcacctggc gcagcgccgc attgcgctgc ttgagcaatc gcttgagatt
    42781 gctccaggcg gtgaaaaatc caggttcgtt gtgaaagcat ccccagtcga ggaatgctct
    42841 tctgtatttg gggccgccgt tgagtaaagt aaacccttct ggcgttatca actgcatcgg
    42901 catcaggtgc gccagttccg cgactttatg cccgtccgta ccgtcaatac ggactttgct
    42961 gtcgccctgt ttgtctttgg ttaagccaat cgccgtctcg cgctcttcgc cctgtaatcg
    43021 cccgtggaga acaaatgcct cctgctcatg gcgaatgacg cgaccaatct gcaaactgcg
    43081 aaacgcccga ccatggccga gcgtatagat ggcttccagc acgctggttt tgccactgcc
    43141 gttggcacct accagaaagt taaagccggg agataaagcg agatccgcgg tttcaatatt
    43201 gcggaaatcg cggatcaaca agcgggtgag ggacattaca gtctcattgg catgacaaca
    43261 taggcagccg actgtgatgc ggcatcttca atctgcacgc tggaaaccga atcggtcagc
    43321 atcatgcgga cgttttcgca tttcagcgcg ttcagaacat ccagcacata gctgacgttg
    43381 aagccgattt ccatctccgc accgctatag gtaacgtcga ggatctcttc cgcttcttcc
    43441 tgttccgggt tgttggcagt aattttcagc tggttttcgc tgacatacag gcgcacaccg
    43501 cggaatttct cgttagagag aatcgccgcg cgagcaaacg cctgcttgag cagatcgcag
    43561 cctgcttcca gatgtttgtc cgggttcttc ggcagaacgc gacggtaatc cgggaagcga
    43621 ccatccacca gtttcgaggt gaaaataaag tcgccaacgt gggcacgaat gttattgctg
    43681 ccaatctgca cacgcagcag attgtcgccg ccgtcgagca tacgcatcag ttcaatcacg
    43741 cctttacgcg gtacgatcac cgaatggctt ggcaaagatt gaccaattgg cattgaacag
    43801 accgccagac ggtggccgtc ggttgccacg gtgcgcagtt cttcaccttc ggtttcaaac
    43861 agcataccgt ttaagtaata gcgaacatcc tgatgcgcca tcgaaaactg ggttgcttca
    43921 atcagacgct tcatcgtcgc ctgcggcagg gtaaattcga cttcgctctg ccagtcatcg
    43981 aggttcggga aatccgccgc tggcagggta gacagcgaaa aacggctacg cccggagcgc
    44041 accagcatcc gttcaccttc cagctgtacg gcaatttccg cgccttcagg cagaccacgg
    44101 cagatatcaa agaatttgcg cgccggaacg gtcgtcgctc ctggctcgtg tggctgaacc
    44161 agcgcaacgc gtgccaccat ttccatctcg agatcagtac cggtcagcga caacgtaccg
    44221 tcagcaacct gtaacagcag attaccgaga atcggtagcg taggacgacc acctaacgga
    44281 ccgctcacct gttgtagcgg ttttaataaa tgctcacgtt ctacggtaaa tttcataggt
    44341 ttacgatgac aatgttctga ttaaatttga aaaatcttct ttgatatcgt ggctctcttc
    44401 acgcaactgc tcgatcttac ggcaggcatg aagcaccgtc gtgtggtcac gcccaccaaa
    44461 cgcatcgcca atctctggca gactgtggtt agtcagctct ttcgctagcg ccatcgccat
    44521 ctggcgcgga cgcgccaccg agcgggatcg acgcttggaa aggagatccg cgactttgat
    44581 cttgtagtac tccgccaccg tcttctgaat attgtcgatg gtgaccagtt tttcctgcaa
    44641 tgccagcaag tcgcgcagcg cctcacgcac gaagtcgatg gtgatcgccc gtccggtaaa
    44701 gttggcgtta gcaataacgc ggttcagcgc cccttccagc tcacgtacgt tagatcgtag
    44761 acgcttggcg ataaagaacg ccacttcacc cggcaaacga atgtcatttt cgtcggcctt
    44821 tttcatcagg atcgccacac gggtttccag ctctggcggt tcgatcgcca cagtcagtcc
    44881 ccaaccgaag cgggatttca aacgatcctc aacgccgttg atctctttcg gatagcgatc
    44941 cgaggtgaga atgatctgtt gattaccttc cagcagggcg ttgaaggtgt ggaaaaactc
    45001 ttcctgagat cgttctttat tagcaaaaaa ctgaatatcg tcgatcagca gtgcatctac
    45061 ggaacggtag tagcgtttaa actcttcgat cgcgttgttt tgcagggctt taaccatgtc
    45121 ctgaacaaag cgctcggagt gcatataaac cactttggca ttcggcttgc gcgccataat
    45181 gccgttaccc accgcatgca gcaggtgagt tttacccaga cccgtgccgc cataaaggaa
    45241 caacgggtta taggcaccgc cagggttatc cgccacctgg cgagccgccg cgcgcgccag
    45301 ttggttagat ttaccttcaa cgaagttatc aaacgtgtgt ttgacgttta cgttagaacg
    45361 ataggtcggt tctgccgggg ccgggacgtt atcccaacct gagcgcgtag aaggcgcagc
    45421 acgttgcggc tgcgtttgcg ccacctgtgc aggggccgcg acgttgctcg tcactgccgc
    45481 ttgtggcgtt tgcgtcaccg gtttggtgcc gacttcaaaa cgcagctgtg gggcatccgc
    45541 tccgcagaaa ctggttagca gtccattgat attattaagg tacttgtccc gtacccaatc
    45601 gaggacaaaa cggtttggcg cgtacaggga cagcgtgtta tcgctcagtt ccgcctgcaa
    45661 tgggcgtatc cacatactga attctgtggc tggtaactca tcctgcaatc gggcaagaca
    45721 ctgctgccaa agcgaaagtg acacggcgga ctccactcga acaaaagtcg ataatgacta
    45781 aggctgaaac attcatgatt gttgacgtac gtcgaaaaga ccctgcttga gggtgacgca
    45841 cgaaccgctg tctgcggtta tatgcccgat caagatcctg caaaacgatc gggaccgcgg
    45901 atcatagcct aaactgcgca agagatcttc tgtttctcac agattttccc tatttatcca
    45961 caggactttc cagaactcgc taagtgtaaa cgatcctgcc gcgaggcggg cacgatttac
    46021 gccgcatatt ggaaaattta atgaccatag acaaaaattg gcttaatcga tctaataaag
    46081 atccaggacg atccttgcgc tttacccatc agcccgtata atcctccacc cggcgcgcca
    46141 tgctggtttc cactggtgtg aggtcgtaca ttttccctgc gaaaaggtgc ggaaaagcgc
    46201 ggtaaataag gaaagagaat tgactccggt gtgtacaatt attacaatcc ggcctcttta
    46261 atcacccatg gcttcggtgt ccatcgtttc atttttcggc ggatatccaa taaagccatt
    46321 gaatttattc aagtttaggt agaaatcgcc atgaaacgca cttttcaacc gtctgtactg
    46381 aagcgcaacc gttctcacgg cttccgtgct cgtatggcta ctaaaaatgg tcgtcaggtt
    46441 ctggcacgtc gtcgtgctaa aggccgcgct cgtctgaccg tttctaagta ataaagctaa
    46501 cccctgagtg gttaagctcg catttcccag ggagttacgc ttgttaactc ccagtcaatt
    46561 cacattcgtc ttccagcagc cacaacgggc tggcacgccg caaattacca ttctcggccg
    46621 cctgaattcg ctggggcatc cccgtatcgg tcttacagtc gccaagaaaa acgttcgacg
    46681 cgcccatgaa cgcaatcgga ttaaacgtct gacgcgtgaa agcttccgtc tgcgccaaca
    46741 tgaactcccg gctatggatt tcgtggtggt ggcgaaaaaa ggggttgccg acctcgataa
    46801 ccgtgctctc tcggaagcgt tggaaaaatt atggcgccgc cactgtcgcc tggctcgcgg
    46861 gtcctgatag ccctcattcg ggtctatcaa cgcctgatta gtccgctact cgggccgcat
    46921 tgtcgtttca ctccaacctg ttcaagctac ggaattgagg cattgcgcag gtttggagtg
    46981 ataaaaggca gttggttgac ggtgaaacgc gtattaaaat gccacccttt acaccctggt
    47041 ggtgacgatc ccgtcccgcc cggaccattt gataccagag aacactaacg atggattcgc
    47101 aacgcaatct tttagtcatc gctttgctgt tcgtgtcttt catgatctgg caagcctggg
    47161 agcaggataa aaacccgcaa cctcaggccc aacagaccac gcagacaacg accaccgcag
    47221 cgggtagcgc cgccgaccag ggcgtaccgg ccagtggcca ggggaaactg atctcggtta
    47281 agaccgacgt gcttgatctg accatcaaca cccgtggtgg tgatgttgag caagctctgc
    47341 tgcctgctta cccgaaagag ctgaactcta cccagccgtt ccagctgttg gaaacttcac
    47401 cgcagtttat ttatcaggca cagagcggtc tgaccggtcg tgatggcccg gataacccgg
    47461 ctaacggccc gcgtccgctg tataacgttg aaaaagacgc ttatgtgctg gctgaaggtc
    47521 aaaacgaact gcaggtgccg atgacgtata ccgacgcggc aggcaacacg tttaccaaaa
    47581 cgtttgtcct gaaacgtggt gattacgctg tcaacgtcaa ctacaacgtg cagaacgctg
    47641 gcgagaaacc gctggaaatc tcctcgtttg gtcagttgaa gcaatccatc actctgccac
    47701 cgcatctcga taccggaagc agcaacttcg cactgcacac cttccgtggc gcggcgtact
    47761 ccacgcctga cgagaagtat gagaaataca agttcgatac cattgccgat aacgaaaacc
    47821 tgaacatctc ttcgaaaggt ggttgggtgg cgatgctgca acagtatttc gcgacggcgt
    47881 ggatcccgca taacgacggt accaacaact tctataccgc taatctgggt aacggcatcg
    47941 tcgctatcgg ctataaatct cagccggtac tggttcagcc tggtcagact ggcgcgatga
    48001 acagcaccct gtgggttggc ccggaaatcc aggacaaaat ggcagctgtt gctccgcacc
    48061 tggatctgac cgttgattac ggttggttgt ggttcatctc tcagccgctg ttcaaactgc
    48121 tgaaatggat ccatagattt gtgggtaact ggggcttctc cattatcatc atcaccttta
    48181 tcgttcgtgg catcatgtac ccgctgacca aagcgcagta cacctccatg gcgaagatgc
    48241 gtatgctgca gccgaagatt caggcaatgc gtgagcgtct gggcgatgac aaacagcgta
    48301 tcagtcagga aatgatggcg ctgtacaaag ctgagaaggt taacccgctg ggcggctgct
    48361 tcccgctgct gatccagatg ccaatcttcc tggcgttgta ctacatgctg atgggttccg
    48421 ttgaactgcg tcaggcaccg tttgcactgt ggatccacga cctgtcggca caggacccgt
    48481 actacatcct gccgatcctg atgggcgtaa cgatgttctt cattcagaag atgtcgccga
    48541 ccactgtgac cgacccgatg cagcagaaga tcatgacctt tatgccggtc atcttcaccg
    48601 tgttcttcct gtggttcccg tcaggtctgg tactgtacta tatcgtcagc aacctggtaa
    48661 ccattattca gcagcagctg atttaccgtg gtctggaaaa acgtggcctg catagccgcg
    48721 agaagaaaaa atcctgattc ggtgagtttt cgctaaaata agggcggtca gttgaccgcc
    48781 ttttttcttt tcgtagggcg gataagcacc gcgcatccgc cacacaaagc aacaggaaca
    48841 tcatgagcga taatgacact atcgtagccc aggccacacc tccgggacgt ggcggcgttg
    48901 gcatcctgcg catctccggc ttcaaagccc gtgaagttgc cgaaaccgtg ctgggtaaac
    48961 tgcctaagcc gcgctacgcc gattatcttc cgtttaaaga cgccgacggc agcgtgctcg
    49021 atcaggggat tgcgctatgg ttccctggcc cgaactcgtt caccggcgaa gatgtgctgg
    49081 aactgcaagg tcatggcggt ccggtgatcc tcgacctgct gttaaaacgc attctgacca
    49141 ttcccggcct gcggattgct cgccctggtg agttttccga acgcgcgttt cttaacgata
    49201 aacttgactt agcccaggcc gaggcgattg ccgatcttat cgacgccagt tcggaacagg
    49261 cggcccgttc ggcacttaac tcgctgcaag gcgcattctc cgcacgggtt aatcatctgg
    49321 tagaagccct cacccacttg cgcatttacg tcgaagcggc aattgatttc cctgatgaag
    49381 agatcgattt cctctccgac ggcaaaattg aagcccagct taatgacgtg atggccgatc
    49441 tcgatgcagt gcgtgctgaa gcacgtcagg gtagtttgtt gcgcgaaggg atgaaagtag
    49501 tgattgccgg acgtcctaat gccggtaaat cgagcctgtt aaacgcgctg gcgggccgtg
    49561 aagcggcaat cgtaaccgat atcgccggaa ccacgcgtga cgtgctgcgt gagcatatcc
    49621 acattgacgg aatgccgctg catatcatcg acactgccgg gctacgtgag gccagtgacg
    49681 aagtggaacg tattggtatc gagcgcgcgt ggcaggaaat tgaacaggcc gaccgcgtgc
    49741 tgtttatggt cgatggcacc acgacagacg ccgttgatcc ggcagagatc tggccggaat
    49801 ttatcgcccg tctgcccgcg aaactgccga tcaccgtggt acgcaataaa gccgatatca
    49861 ccggcgaaac gctgggaatg agtgaagtga acggtcacgc gttaattcgt ctctcggcaa
    49921 ggactggtga aggcgtggac gtgctgcgta accatctcaa acagagcatg ggctttgaca
    49981 ccaatatgga aggcggcttc ctggcgcgcc gtcgccactt acaggcgctg gaacaggcag
    50041 cggagcattt gcagcaaggt aaagcgcaac tgttgggagc ctgggcaggt gaactgctgg
    50101 cggaagagtt gcgcctggca cagcagaact taagcgaaat caccggggaa tttacttcag
    50161 acgacctgct ggggcggatt ttctccagct tctgtattgg taagtaaccg cgcttacgaa
    50221 gccgtattct gactgtcaga tgcggcttcg cttcattgtt agcactcctg ttatgcctca
    50281 accctttttt aaacattaaa attcttacgt aatttataat ctttaaaaaa agcatttaat
    50341 actgctcccc gaacgattgt gattcgattc acatttaaac aatttcagaa tagacaaaaa
    50401 ttctgagtgt aataatgtag cggcgtgtct ttcgaggata agtgcattat gaatatctta
    50461 catgtatgtg tgacctcaaa atggttcaat atcgacaata agattgtcga tcaccgccct
    50521 taatttaccc ttctgtagcc atcaccagag ccaaaccgat tagattcaat gtgatcgatt
    50581 agtttgctat atcttaattt tgccttttgc aaaggccatc tctcgtttat ttacttattt
    50641 tagtaaatga tggtgcttgt atatatacat ggtgaattaa tggatattgc agatgtaata
    50701 ttcacaggga tcactgtaat taaaataaat gaaggattat gtaatggaaa actttaaaca
    50761 tctccctgaa ccgttccgca ttcgtgttat tgagccagta aaacgtacta ctcgcgctta
    50821 tcgtgaagaa gcaattatta aatccggtat gaacccgttc ctgctggata gcgaagatgt
    50881 gtttatcgat ttactgaccg acagcggcac cggggcggta acccaaagta tgcaggcagc
    50941 gatgatgcgc ggcgacgaag cctacagcgg cagccgcagc tactatgcgt tagccgagtc
    51001 agtgaaaaat atctttggtt atcaatatac tattccgact caccagggcc gtggcgcaga
    51061 gcaaatctat attccggtac tgattaaaaa acgcgagcag gaaaaaggcc tggatcgcag
    51121 caaaatggtg gcattctcta actatttctt tgataccacg cagggccata gccagattaa
    51181 cggctgtacc gtgcgtaacg tctatatcaa agaagccttc gatacgggcg tgcgttacga
    51241 ctttaaaggc aactttgacc tcgaaggatt agaacgcggt attgaagaag ttggcccgaa
    51301 taacgtgccg tatatcgttg caaccatcac cagtaactct gcaggtggtc agccggtttc
    51361 actggcaaac ttaaaagcga tgtacagcat cgcgaagaaa tacgatattc cggtggtaat
    51421 ggactccgca cgctttgctg aaaacgccta tttcatcaag cagcgtgaag cagaatacaa
    51481 agactggacc atcgagcaga tcacccgcga aacctacaaa tatgccgata tgctggcgat
    51541 gtccgccaag aaagatgcga tggtgccgat gggcggcttg ctgtgcatga aagacgacag
    51601 cttctttgat gtgtacaccg agtgcagaac cctttgcgtg gtgcaggaag gcttcccgac
    51661 atatggcggc ctggaaggcg gcgcgatgga gcgtctggcg gtaggtctgt atgacggcat
    51721 gaatctcgac tggctggctt atcgtatcgc gcaggtacag tatctggtcg atggtctgga
    51781 agagattggc gttgtctgcc agcaggcggg cggtcacgcg gcattcgttg atgccggtaa
    51841 actgctgccg catatcccgg cagaccagtt cccggcacag gcgctggcgt gcgagctgta
    51901 taaagtcgcc ggtatccgtg cggtagaaat tggctctttc ctgttaggcc gcgatccgaa
    51961 aaccggtaaa caactgccat gcccggctga actgctgcgt ttaaccattc cgcgcgcaac
    52021 atatactcaa acacatatgg acttcattat tgaagccttt aaacatgtaa aagagaacgc
    52081 ggcgaatatt aaaggattaa cctttaccta cgaaccaaaa gtattgcgtc acttcaccgc
    52141 aaaactgaaa gaagtttaat taatactaca gagtggctat aaggatgtta gccactctct
    52201 taccctacat cctcaataac aaaaatagcc ttcctctaaa ggtggcatca tgactgatca
    52261 agctgaaaaa aagcactctg cattttgggg tgttatggtt atagcaggta cagtaattgg
    52321 tggaggtatg tttgctttac ctgttgatct tgccggtgcc tggtttttct ggagtgcctt
    52381 tatccttatc attgcctggt tttcaatgct tcattccggg ttattgttat tagaagcaaa
    52441 tttaaattat cctgtcggct ccagttttaa caccatcacc aaagatttaa taggtaacac
    52501 ctggaacatt atcagcggta ttaccgttgc cttcgttctc tatatcctca cttatgccta
    52561 tatctctgct aatggtgcga tcattagtga aacgatatca atgaatttgg gctatcacgc
    52621 taatccacgt attgtcggga tctgcacagc cattttcgtt gccagcgtat tgtggataag
    52681 ttcgttagcc gccagtcgca ttacctcatt gttcctcggg ctgaagatta tctcctttgt
    52741 gatcgtgttt ggttctttct tcttccaggt cgattactcc atcctgcgcg atgccaccag
    52801 caccactgcg ggaacgtctt acttcccgta tatctttatg gctttgccgg tgtgtctggc
    52861 gtcatttggt ttccacggca atattcccag cctgattatt tgctatggca aacgcaaaga
    52921 taagttaatc aaaagcgtgg tatttggttc gc
//
LOCUS       DAJTYG010000018        49571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTYG010000018 DAJTYG010000000
VERSION     DAJTYG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.018"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..1224
                     /locus_tag="OO568_001138"
     CDS             <1..1224
                     /locus_tag="OO568_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6640574.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGIIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="OO568_001139"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="OO568_001139"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6640575.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVICEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="OO568_001140"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="OO568_001140"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR6640576.1"
                     /translation="MSTLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="OO568_001141"
     CDS             2714..3427
                     /locus_tag="OO568_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6640577.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="OO568_001142"
     CDS             complement(3399..4076)
                     /locus_tag="OO568_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6640578.1"
                     /translation="MLTLNKISYRWPGAATDCLCGISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="OO568_001143"
     CDS             complement(4070..4747)
                     /locus_tag="OO568_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6640579.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5118)
                     /locus_tag="OO568_001144"
     CDS             complement(4735..5118)
                     /locus_tag="OO568_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6640580.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5134..5442)
                     /locus_tag="OO568_001145"
     CDS             complement(5134..5442)
                     /locus_tag="OO568_001145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640581.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5443..6021)
                     /locus_tag="OO568_001146"
     CDS             complement(5443..6021)
                     /locus_tag="OO568_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6640582.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="OO568_001147"
     CDS             complement(6044..6475)
                     /locus_tag="OO568_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6640583.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="OO568_001148"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="OO568_001148"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6640584.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="OO568_001149"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="OO568_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6640585.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="OO568_001150"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="OO568_001150"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6640586.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="OO568_001151"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="OO568_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6640587.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="OO568_001152"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="OO568_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6640588.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQHIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="OO568_001153"
     CDS             12291..13031
                     /locus_tag="OO568_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6640589.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="OO568_001154"
                     /pseudo
     CDS             13198..14148
                     /locus_tag="OO568_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="OO568_001155"
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="OO568_001155"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCR6640590.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="OO568_001156"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="OO568_001156"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6640591.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="OO568_001157"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="OO568_001157"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6640592.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="OO568_001158"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="OO568_001158"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6640593.1"
                     /translation="MPNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLGKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKMDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMTEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRAARDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVAGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="OO568_001159"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="OO568_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6640594.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="OO568_001160"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="OO568_001160"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6640595.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="OO568_001161"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="OO568_001161"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6640596.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="OO568_001162"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="OO568_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6640597.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="OO568_001163"
     CDS             23768..23827
                     /locus_tag="OO568_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6640598.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23924..24526)
                     /locus_tag="OO568_001164"
     CDS             complement(23924..24526)
                     /locus_tag="OO568_001164"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6640599.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24547..24730)
                     /gene="ssrS"
                     /locus_tag="OO568_001165"
     ncRNA           complement(24547..24730)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO568_001165"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24772..25101)
                     /gene="zapA"
                     /locus_tag="OO568_001166"
     CDS             complement(24772..25101)
                     /gene="zapA"
                     /locus_tag="OO568_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6640600.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25269..25847
                     /gene="ygfB"
                     /locus_tag="OO568_001167"
     CDS             25269..25847
                     /gene="ygfB"
                     /locus_tag="OO568_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6640601.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25873..27198
                     /gene="pepP"
                     /locus_tag="OO568_001168"
     CDS             25873..27198
                     /gene="pepP"
                     /locus_tag="OO568_001168"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6640602.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHARGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27195..28373
                     /gene="ubiH"
                     /locus_tag="OO568_001169"
                     /gene_synonym="visB"
     CDS             27195..28373
                     /gene="ubiH"
                     /locus_tag="OO568_001169"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6640603.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28396..29598
                     /gene="ubiI"
                     /locus_tag="OO568_001170"
                     /gene_synonym="visC"
     CDS             28396..29598
                     /gene="ubiI"
                     /locus_tag="OO568_001170"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6640604.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLSEWLR"
     gene            complement(29672..29755)
                     /locus_tag="OO568_001171"
     CDS             complement(29672..29755)
                     /locus_tag="OO568_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640605.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30046..31140
                     /gene="gcvT"
                     /locus_tag="OO568_001172"
     CDS             30046..31140
                     /gene="gcvT"
                     /locus_tag="OO568_001172"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6640606.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31164..31553
                     /gene="gcvH"
                     /locus_tag="OO568_001173"
     CDS             31164..31553
                     /gene="gcvH"
                     /locus_tag="OO568_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6640607.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31672..34545
                     /gene="gcvP"
                     /locus_tag="OO568_001174"
     CDS             31672..34545
                     /gene="gcvP"
                     /locus_tag="OO568_001174"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6640608.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEEIGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34812..35555
                     /locus_tag="OO568_001175"
     CDS             34812..35555
                     /locus_tag="OO568_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6640609.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGRSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35612..37045)
                     /gene="bglA"
                     /locus_tag="OO568_001176"
     CDS             complement(35612..37045)
                     /gene="bglA"
                     /locus_tag="OO568_001176"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6640610.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37090..37401
                     /locus_tag="OO568_001177"
     CDS             37090..37401
                     /locus_tag="OO568_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR6640611.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            37565..38224
                     /locus_tag="OO568_001178"
     CDS             37565..38224
                     /locus_tag="OO568_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6640612.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38420..39400)
                     /gene="ygfZ"
                     /locus_tag="OO568_001179"
     CDS             complement(38420..39400)
                     /gene="ygfZ"
                     /locus_tag="OO568_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6640613.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39643..39909
                     /gene="sdhE"
                     /locus_tag="OO568_001180"
     CDS             39643..39909
                     /gene="sdhE"
                     /locus_tag="OO568_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6640614.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39890..40297
                     /locus_tag="OO568_001181"
     CDS             39890..40297
                     /locus_tag="OO568_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR6640615.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40337..40858)
                     /gene="fldB"
                     /locus_tag="OO568_001182"
     CDS             complement(40337..40858)
                     /gene="fldB"
                     /locus_tag="OO568_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6640616.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40970..41866
                     /gene="xerD"
                     /locus_tag="OO568_001183"
     CDS             40970..41866
                     /gene="xerD"
                     /locus_tag="OO568_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6640617.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLLMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41891..42601
                     /gene="dsbC"
                     /locus_tag="OO568_001184"
     CDS             41891..42601
                     /gene="dsbC"
                     /locus_tag="OO568_001184"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6640618.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42607..44340
                     /gene="recJ"
                     /locus_tag="OO568_001185"
     CDS             42607..44340
                     /gene="recJ"
                     /locus_tag="OO568_001185"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6640619.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44431..45529
                     /gene="prfB"
                     /locus_tag="OO568_001186"
     CDS             join(44431..44505,44507..45529)
                     /gene="prfB"
                     /locus_tag="OO568_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6640620.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45539..47056
                     /gene="lysS"
                     /locus_tag="OO568_001187"
     CDS             45539..47056
                     /gene="lysS"
                     /locus_tag="OO568_001187"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6640621.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47099..47647)
                     /gene="idi"
                     /locus_tag="OO568_001188"
     CDS             complement(47099..47647)
                     /gene="idi"
                     /locus_tag="OO568_001188"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6640622.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARILLSAFTQLK"
     gene            47702..47773
                     /locus_tag="OO568_001189"
     CDS             47702..47773
                     /locus_tag="OO568_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640623.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47770..47895)
                     /locus_tag="OO568_001190"
     CDS             complement(47770..47895)
                     /locus_tag="OO568_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640624.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47897..49345)
                     /gene="uacT"
                     /locus_tag="OO568_001191"
     CDS             complement(47897..49345)
                     /gene="uacT"
                     /locus_tag="OO568_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6640625.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11966 a        12405 c        13349 g        11851 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg ggattattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggccgcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tctgcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg tcgactctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gagtgcataa accgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcagacgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggtgctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agtcgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ctatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgccgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggcgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgaccacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc caggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgggta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagcca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctagat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 tgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcccttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcatt gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ttggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcacattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgtgattg ggctttctcg tttctcgccg ttataaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggtgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgcg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagcc
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgtaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ctaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttattgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgacgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccgctacg tgaacgtcgt tttctacggc caggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggc ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcggtca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tccattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttaccc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttaggcat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gttaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcacagggga ggagcaatcc tcccttaccc ttactcacta aattaggtta
    23881 aagaatcaac gatgtcaatc agggcgatgc aggtgtatcg cccttaccac tcccagactt
    23941 tcgacggtgt aaccaccgca ggaagaggga tatcccactc ttcaacgggg agtttttcca
    24001 ccaactgaca atcatgcgca taacccaccg gttgcgtttt atagtgctgc cagttttgta
    24061 aggtccgatc ataaaaacca ccgcccattc ccaggcgctg accgtactca tcaaaggcga
    24121 ccagcggtgt gatcagcacg tctaatcggg aaaggggtag cacgtcacgc acatccagtt
    24181 ttggctcatg gatcttcaac ctgttcatca ccagttcgct ttgcggatgg taattcagga
    24241 acagcaaatt accggcacta aagggatgca aaactggaag atatacgcgc ttaccggcgc
    24301 gccagagttg ttctattagc ggctgggtgt cgagttcacc atcaaaagag aggaatacag
    24361 cgaccgtatg cgccatcacc accgggggat aagtcatcat ccgggtagcg gcttgttgac
    24421 ccatttccag ctgttgttcc ggcgttaacg cacgacgacg ttgccgaatc attttgcgga
    24481 tttcttgtcg ggataatgtc agtgggagct ctggtagttg cgtcatggct ggtgccagat
    24541 aagaagggaa tctccgagat gccgccgcag gctgtaaccc ttgaaccctt ggttcaaggt
    24601 gaatgtgtcg tcgcagtttt aaggcttctc ggacgaaccg agcatgctca ccaaccgcgg
    24661 agcgccacat tcttgtggta tgaaatatcg gctcagggga ctggcccgct tgcgaacatc
    24721 tcagagaaat tttgtcttca cggttactct accacagtaa accgaaaagt gtcattcaaa
    24781 gttttggtta gttttttcgg tgatgcgacc ttgttcaagt aacgcttgtt ctatggtctg
    24841 ctgcagcatc cgaatacgct gttccatact tgccgcgtag tcacgagtct ttgctttttc
    24901 ttgcgctaac tcatagctga tattcaatgc ggcaatgaag accaactgtt cagtatttgt
    24961 gactctagtg cgttctttca gatcttgcaa ccgttggttc agatcgtccg ctgcctgatt
    25021 caacgcatcc ctttggtcag gcgggcagtt cacacgcagt gaacggccaa aaatttggat
    25081 atcgacgggt tgtgcagaca tgccaccttc ctgctgattg actgcgctgc cttcgtcttt
    25141 agacccatgg tctgcgaagg ggcgacgcta tagctaccct gatgagaaga gacaagccct
    25201 tttctggtcc accagggccc aaagtggtag catatcatga atattcctcc ctttgacgac
    25261 gaatgcttat gtctatacag aacgaaatgc ctggttacaa cgaaatgaac cagtatctga
    25321 accaacaagg gacgggtctg acccctgccg agatgcatgg tttaatcagt gggatgatat
    25381 gtggcggtaa cgatgacagc tcatggctgc cgctacttca cgacctgacg aacgaaggca
    25441 tggctttcgg tcatgagctg gcacaggcac tgcgtaaaat gcactctgcc accagcgatg
    25501 ccctgcagga tgacggcttc ctttttcagc tttatctgcc tgatggcgat gatgtcagcg
    25561 ttttcgatcg ggctgatgca ctggcaggtt gggtcaatca cttcctgctt ggtcttggcg
    25621 ttacgcaacc gaagctggac aaagtgaccg gcgaaaccgg tgaagccatc gacgatctgc
    25681 gtaacatcgc gcagctgggc tacgacgaag acgaagatca ggaagagctt gaaatgtcgc
    25741 ttgaagagat catcgaatac gttcgtgttg ccgcgctgtt atgccacgac acctttactc
    25801 atccgcaacc gaccgcgcca gaagtacaaa aaccgactct acactaaaaa caaaaaacgt
    25861 aaggagagtg ttatgagtga gatatcccgg caagagtttc agcgtcgccg tcaggccctg
    25921 gtggagcaaa tgcaacccgg cagcgccgcg ctgatttttg ctgcaccaga agtaacacgt
    25981 agcgccgaca gcgaataccc ctatcgtcag aacagtgact tctggtactt caccggcttt
    26041 aacgaaccgg aagcggtgct ggtgctgatt aaaagcgatg acactcataa ccacagcgtt
    26101 ctgtttaacc gcgttcgcga cctgacggcg gagatctggt ttggccgtcg cttaggccag
    26161 gatgccgcgc cagagaaact gggcgttgac cgcgcactgg cattcagcga aatcaatcag
    26221 caactttatc aactacttaa cggcctggat gtggtttacc atgcccgggg cgaatatgca
    26281 tatgctgatg aaatcgtgaa cagtgcgctg gaaaaactgc gtaaaggttc gcggcaaaat
    26341 ctcaccgcac cggcaacgat gatcgactgg cgtcctgttg ttcatgaaat gcgcctgttc
    26401 aaatcgccag aagagattgc cgtactccgc cgcgcgggag aaatcaccgc catggcacat
    26461 acacgggcga tggaaaaatg ccgtccggga atgttcgagt accatctgga aggcgaaatt
    26521 caccacgaat ttaaccgcca cggtgcgcgc tatccttcct ataacaccat tgtaggcagc
    26581 ggtgaaaacg gctgcattct gcactacacc gaaaacgaga gtgaactgcg cgacggtgac
    26641 ctggtgttga ttgacgcggg ttgtgaatac aaaggttacg cgggcgatat tacccgcacc
    26701 ttcccggtca acggcaaatt cacccaggcc cagcgtgaaa tctacgacat tgtgctggag
    26761 tctctcgaaa ccagcctgcg cctgtatcgt ccgggaactt ccattctgga agtcactggt
    26821 gaagtggtgc gcatcatggt tagcggcctg gtaaaactcg gcatcctgaa aggtaatgtt
    26881 gatgaactga tcgctcagaa cgcccatcgt cctttcttta tgcatggcct tagccactgg
    26941 ttaggactgg atgtccatga cgttggcgtt tatggtcagg atcgctcgcg cattctggaa
    27001 ccggggatgg tactgaccgt agagcctggt ctgtatatcg cgccggatgc ggatgtgcca
    27061 gaacaatatc gcggtatcgg cattcgtatt gaagacgaca ttgtgattac cgaaaccggc
    27121 aacgaaaacc tcaccgccag cgtggtgaaa aagccggaag aaatcgaagc gttgatggct
    27181 gctgcgagaa agcaatgagc gtaatcatcg tcggtggcgg catggcgggc gcgacgctgg
    27241 cgctggctat ttcccggtta agtcacgggg cgctgccggt acatttgatt gaagcgactg
    27301 cgccagagtc acatgctcat ccgggctttg atggacgagc gatagcgctg gcggcgggta
    27361 cctgtcagca actggcgcgc atcggcgtct ggcaatctct ggctgattgc gcaaccgcca
    27421 tcactaccgt gcatgtcagc gatcgtggtc atgctggatt cgtcaccctc gccgcagaag
    27481 attaccaact ggcggcgctg gggcaggttg tcgaattgca taatgttggg caacggctgt
    27541 ttgcattgct gcgaaaagca cctggcgtaa tgctgcattg ccccgatcgc gtagctaacg
    27601 ttgcccgtac tcagagtcac gttgaagtga cgctggagag cggcgagatg ctgacgggcc
    27661 gcgtgctgat agccgctgat ggcacccatt cagcgttagc caccgcgtgc ggcgttgatt
    27721 ggcagcagga gccttacgaa caactggcag tgattgccaa cgttgctact tctgttgcgc
    27781 acgaaggccg cgcttttgaa cgcttcacgc aacatggccc gctggcgatg ttgccgatgt
    27841 ctgacggacg ctgttcgctg gtctggtgtc atccactgga acggcgcgaa gaggtgctgt
    27901 cgtggagtga cgagaagttt tgccgtgaac tccagtctgc ctttggctgg cgactggggc
    27961 aaattaccca cgccggtaaa cgcagtgctt atccgctggc attaacccgc gccgccagac
    28021 cgattaccca tcgcactgtg ctggtgggca atgcggcgca aactctgcac ccgattgccg
    28081 ggcaagggtt taacctcggt atgcgagatg tcatgagcct tgcggaaacc ctgactcagg
    28141 cgcatgagcg cggagaagac atcggtgatt acggcatatt gtgccgctat cagcagcgtc
    28201 gacagagcga tcgcgaagca accatcggcg tcacggacag cctggtacat ctttttgcca
    28261 accgttgggc accgctggtt gtcgggcgca acatcggact gatgacgatg gaattattca
    28321 ccccggcacg cgatgtgctg gcgcagcgca ccctcggttg ggtggcgcgt tgaaggcggt
    28381 tttaaggaat aaacaatgca aagtgttgat gtagccattg ttggcggcgg catggtgggg
    28441 ctggcggttg cctgcggctt acaggggagc ggcttacgcg ttgccgtact ggagcagcgc
    28501 gtaccggaac ctctggcggt agatgcacta ccacaactgc gcgtttcggc tatcaatgcc
    28561 gccagcgaaa aattactcac ccgtcttggc gtctggcagg acattctcgc tcgtagggcc
    28621 agttgctatc acggcatgga agtgtgggac aaagacagtt ttggtcacat ctcgtttgac
    28681 gatcaaagca tgggctatag ccatcttggg cacatcgttg aaaactcagt gattcactac
    28741 gcgctgtgga acaaagcgca gcagtcgtca gatattactc tgttggcccc cacagaatta
    28801 cagcaggtcg cctggggaga aaatgaaacc ttcctgacgc tgaaagatgg cagcatgtta
    28861 acggcgcgtc tggtgattgg cgcggacggt gctaattcct ggttgcgcaa caaagcagat
    28921 attccgctga ctttctggga ttatcagcat cacgcgctgg tagcgaccat tcgcacggaa
    28981 gaaccgcatg atgcggtggc gcggcaggtt ttccatggcg aaggcattct ggccttttta
    29041 ccgcttagcg atccgcatct ttgctcgatt gtctggtcac tgtcgccaga ggaagcgcag
    29101 cggatgcagc aggcaagtga agacgaattt aatcgcgcgt taaatatcgc ttttgataat
    29161 cgccttggct tatgcaaggt tgagagcgcg cgtcaggtgt tcccgctgac ggggcgttat
    29221 gcgcgccagt ttgccgcaca ccgtctggcg ctggtgggcg acgccgcgca taccattcac
    29281 ccactggcgg ggcagggcgt taatctcgga tttatggacg ccgcagagct gattgctgaa
    29341 ctgaaacggt tgcatcgtca gggtaaagac atcgggcagt acatttacct gcgccgctat
    29401 gaacgtagcc gcaagcacag cgcggcgctg atgctggctg gtatgcaggg ttttcgcgat
    29461 ctcttttccg gcgctaatcc ggcgaaaaaa ctgctgcgtg atattggttt gaaactggcc
    29521 gacacgcttc ctggcgttaa gccgcaactt atccgccagg caatgggatt aaacgatttg
    29581 tctgaatggc tgcgttaaaa atttctcctc tgttgtttat ttgataccca tcacactttc
    29641 atctcccggt tttttcgccg ggagattttc ctcatttgaa ataaactaat ttcacctccg
    29701 ttttcgcatt atattttcta atgccatgat tttttgattt agtgtttttt gacatttttt
    29761 tagctcttaa tattgtctta ttcaaattga ctttctcatc acatcatctt tgtatagaaa
    29821 ttggtgtatt ttttggtttt ttattctgtc gcgatttttg cattttttaa ccataagcta
    29881 atgtgatgat caattttacc ttatggttaa cagtctgttt cggtggtaag ttcaggcaaa
    29941 agagaacgat tgcgttgggg accgggagtg gctccgatgc tgggttttgt ggtgataatt
    30001 tcaccatgaa aaagttgtca gccccgctta ttcaatgagg acaagatggc acaacagact
    30061 cctttgtacg aacaacacac gctttgcggc gctcgcatgg tggatttcca cggctggatg
    30121 atgccgctgc attacggttc gcaaatcgac gaacatcatg cggtacgtac cgatgccgga
    30181 atgtttgatg tgtcgcatat gaccatcgtc gatctccgcg gcagccgcac ccgggagttt
    30241 ctgcgttatc tgctggcgaa cgatgtggcg aagctcacca aaagcggcaa agccctttac
    30301 tcggggatgt tgaatgcctc tggcggtgtg atagatgatc tcatcgtcta ctactttact
    30361 gaagatttct tccgcctcgt tgttaactcc gccacccgcg aaaaagacct ctcctggatt
    30421 acccaacacg ctgaaccttt cggcatcgaa attaccgttc gtgatgacct ttccatgatt
    30481 gccgtgcaag ggccgaatgc tcaggcaaaa gctgccacac tgtttaatga tgctcagcgt
    30541 caggcggtgg aagggatgaa accgttcttt ggcgtgcagg tgggcgatct gtttattgcc
    30601 accactggtt ataccggtga agcgggctat gaaattgcgc tgcccaatga aaaagcggcc
    30661 gatttctggc gtgcgctggt ggaagcgggc gttaagccct gcggtctggg ggcgcgtgac
    30721 acgctgcgtc tggaagcggg catgaatctt tatggtcagg agatggacga aactatttct
    30781 cctttagccg ccaacatggg ctggactatc gcctgggaac cgacagatcg tgactttatc
    30841 ggtcgtgaag ccctggaagt gcagcgtgag catggtacag aaaaactggt tggtctggtg
    30901 atgaccgaaa aaggcgtgct gcgtaatgaa ctgccggtac gctttaccga tgcgcagggc
    30961 aaccagcatg aaggcattat caccagcggt actttctccc cgacgctggg ttacagcatt
    31021 gcgctggcgc gcgtgccgga aggtattggc gaaacggcga ttgtgcaaat tcgcaaccgt
    31081 gaaatgccgg ttaaagtgac aaaacctgtt tttgtgcgta acggcaaagc cgtcgcgtga
    31141 tttacttttt tggagattga ttgatgagca acgtaccagc agaactgaaa tacagcaaag
    31201 aacacgaatg gctgcgtaaa gaagccgacg gcacttacac cgttggtatt accgaacatg
    31261 ctcaggagct gttaggcgat atggtgtttg ttgacctgcc ggaagtgggc gcaacggtta
    31321 gcgcgggcga tgactgcgcg gttgccgaat cggtaaaagc ggcgtcagac atttatgcgc
    31381 cagtaagcgg tgaaatcgtg gcggtaaacg acgcactgag cgattccccg gaactggtga
    31441 acagcgaacc gtatgcaggc ggctggatct ttaaaatcaa agctagcgat gaaagcgaac
    31501 tggaatcact gctggatgcg accgcatacg aagcattgtt agaagatgag taacggcttt
    31561 attcctcttc tgcgggagag gatcagggtg aggaaaattt atgcctcacc ctcactctct
    31621 tcgtaaggag agaggttcac aattcactgc acgtttcagg aaccatcgct catgacacag
    31681 acgttaagcc agcttgaaaa cagcggcgct tttattgaac gccatatcgg accggacgcc
    31741 gcgcaacagc aagaaatgct gaatgccgtt ggtgcacaat cgttaaacgc gctgaccggc
    31801 cagattgtgc cgaaagatat tcagcttgcg acaccaccgc aggttggcgc accggcgacc
    31861 gaatacgccg cactggcaga actcaaggct attgccagtc gcaataaacg cttcacgtct
    31921 tacatcggca tgggttacac cgccgtgcag ctaccgccgg ttatcctgcg taacatgctg
    31981 gaaaatccgg gctggtatac cgcgtacact ccgtatcaac ctgaagtctc ccagggccgc
    32041 cttgaagcac tgctcaactt ccagcaggta acgctggatt tgactggact ggatatggcc
    32101 tctgcttctc ttctggacga ggccaccgct gccgccgaag caatggcgat ggcgaaacgc
    32161 gtcagcaaac tgaaaaatgc caaccgcttc ttcgtggctt ccaatgtgca tccgcaaacg
    32221 ctggatgtgg tccgtactcg tgccgaaacc tttggttttg aagtgattgt cgatgacgcg
    32281 caaaaagtgc tcgaccatca ggacgtcttc ggcgtgctgt tacagcaggt aggcactacc
    32341 ggtgaaattc acgactacac tgcgcttatt agcgaactga aatcacgcaa aattgtggtc
    32401 agcgttgccg ccgatattat ggcgctggtg ctgttaactg cgccgggtaa acagggcgcg
    32461 gatattgttt ttggttcggc gcaacgcttc ggcgtgccga tgggctacgg tggcccacac
    32521 gcggcattct ttgcggcgaa agatgaatac aaacgctcaa tgccgggccg tattatcggt
    32581 gtatcgaaag atgcagctgg caataccgcg ctgcgcatgg cgatgcagac tcgcgagcaa
    32641 catattcgtc gtgagaaagc gaactccaac atttgtactt cccaggtact gctggcaaac
    32701 atcgccagcc tgtatgccgt ttatcacggc ccggttggcc tgaaacgtat cgctaaccgc
    32761 attcaccgtc tgaccgatat tctggcggcg ggcctgcaac aaaaaggtct gaaactgcgc
    32821 catgcgcact atttcgacac cttgtgtgtg gaagtggccg acaaagcggg cgtactgacg
    32881 cgtgccgaag cggctgaaat caacctgcgt agcgatatcc tgaacgccgt ggggatcacc
    32941 cttgatgaaa ccaccacgcg tgaaaacgta atgcagcttt tcagtgtgct gctgggcgat
    33001 aaccacgggc tggagatcga cacgctggac aaagacgtgg ctcacgacag ccgctctatc
    33061 cagcctgcga tgctgcgcga cgacgaaatc ctcacccatc cggtgtttaa tcgctatcac
    33121 agcgaaaccg aaatgatgcg ctatatgcac tcgctggagc gtaaagatct ggcgctgaat
    33181 caggcgatga tcccgctggg ttcctgcacc atgaaactga acgccgccgc cgagatgatc
    33241 ccaatcactt ggacggaatt tgccgaactg cacccgtttt gcccgccgga acaggctgaa
    33301 ggttatcagc agatgattgc gcagctggct gactggctgg tgaaactgac cggttacgac
    33361 gccgtttgta tgcagccgaa ctccggcgca cagggcgaat acgcgggcct gctggcgatt
    33421 cgtcattatc atgaaagccg caacgaaggg catcgcgata tctgcctgat cccggcttct
    33481 gcgcacggaa ctaaccccgc ttctgcacat atggcaggaa tgcaggtggt ggttgtggcg
    33541 tgtgataaaa acggcaacat cgatctgact gatctgcgcg cgaaagcgga acaggcgggc
    33601 gataacctct cctgtatcat ggtgacttat ccttctaccc acggcgtgta tgaagaaacg
    33661 atccgtgaag tgtgtgaagt cgtgcatcag ttcggcggtc aggtttacct tgatggcgcg
    33721 aacatgaacg cccaggttgg catcacctcg ccgggcttta ttggcgcgga cgtttcgcac
    33781 ctcaacctgc ataaaacttt ctgcattccg cacggcggtg gtggtccggg tatgggaccg
    33841 atcggtgtga aagcgcatct ggcaccgttt gtaccgggtc atagcgtggt gcaaatcgaa
    33901 ggcatgttaa cccgtcaggg cgcagtttct gcggcaccgt tcggtagcgc ctctatcctg
    33961 ccaatcagct ggatgtacat ccgcatgatg ggcgcagaag ggctgaaaaa agcaagccag
    34021 gtggcaatcc tcaacgccaa ctatattgcc agccgcctgc aggatgcctt cccggtgctg
    34081 tataccggtc gcgacggtcg cgtggcgcac gaatgtattc tcgatattcg cccgctgaaa
    34141 gaagaaatcg gcatcagcga gctggatatt gccaagcgcc tgatcgacta cggtttccac
    34201 gcgccgacga tgtcgttccc ggtggcgggt acgctgatgg ttgaaccgac tgaatctgaa
    34261 agcaaagtgg aactggatcg ctttatcgac gcgatgctgg ctatccgcgc agaaattgac
    34321 caggtgaaag ccggtgtctg gccgctggaa gataacccgc tggtgaacgc gccgcacatt
    34381 cagagcgaac tggtcgccga gtgggcgcat ccgtacagcc gtgaagttgc ggtattcccg
    34441 gcaggcgtgg cagacaaata ctggccgacg gtgaaacgtc tggatgatgt ttacggcgac
    34501 cgtaacctgt tctgctcctg cgtaccgatt agcgaatacc agtaattcac tgattcgagt
    34561 attttctaaa ggcgcttcgg cgccttttta gtcagatgac aaagtacaaa agtgctcaga
    34621 cagtcccctc gcccctttgg ggagagggtt agggtgaggg gaacaggccg gcactggcgc
    34681 gaatatttac cctcaccccg accatctccc tgaaagggcg agggggaaaa gcgtgccaac
    34741 attgaagatt gagccagttt gttagcaatc tcaaagatac gtcaacgaat taatttttct
    34801 cggaaaaaca aatggctata gcacttgtga ccggtggcag tcgcggtatc gggcgggcaa
    34861 ctgcattact gttggcgcaa gaagggtata cggtggcggt taattatcag caaaacctcc
    34921 acgcggcgca ggaagtgatg aacttaataa tgcaagccgg tggcaaagca ttcgtgctcc
    34981 aggcggatat cagcgacgaa aaccaggtcg ttgcgatgtt tacagcaatc gatcagcacg
    35041 atgaaccgct agcagcgctg gtcaataacg ccgggatctt gtttacccag tgcaccgttg
    35101 aaaaccttac cgcagagcga atcaaccgag tactttccac caacgtgacg ggatattttc
    35161 tctgctgccg cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt ggcggcgcta
    35221 tcgtcaatgt ctcttcggtg gcctcacggt tgggttcgcc aggggaatat gttgattacg
    35281 cggcatcgaa aggggcgatt gatacgttaa ccaccggacg atcgctggaa gtcgccgcgc
    35341 aggggatccg cgttaactgc gtgcggccag ggtttattta taccgaaatg cacgccagcg
    35401 gcggcgagcc tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag cgtggtggac
    35461 aggcagaaga ggtcgcgcag gccattgtct ggctactaag tgataaagcc tcttacgtca
    35521 cgggaagttt tatcgatttg gcgggcggga aataaaacag ggaagttgtc tgaccggatg
    35581 caacaagtat tgcatccggt acttcatcga cttaaagctt ctcgccgttg ctggcaatca
    35641 cctctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca gtaccgtcgt
    35701 catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggta gtgaatgaca
    35761 cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc cacaccatcg taagtcactg
    35821 cttttttcat ctcttcaata tgggcgcgca ggtagtcaat gcggtagtcg tcgttgatgc
    35881 tgccatcttc ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata aacagcggcc
    35941 tctgataacg ctcatacagt tcgcaaaggg catagcgcag acctactgga tcaatctgcc
    36001 agccccagtc agacgcttta acatacgggt tcggtacgct gccttcaaaa ccagagatcg
    36061 catcgccggt gccgccttcg gccttcactg cgttggtcat gtaatagctg aaaccaagat
    36121 aatcgcaggt gccttcgcgc agtacatcga gatcgccgtc ttccattttg atattaaatc
    36181 cgcgacgttc ccactcgttc aatacatagg acgggtaata gccgcatagc tgcacatcgg
    36241 taaagacgta gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc gggttacatg
    36301 agtagggata gagcggcacc atcgccagca tacagccgac tttcatctcc gggttaatgc
    36361 gacgcgcggc tttcactgcc agggcgctgg cgacaaactg gtgatgcagc acctggtaca
    36421 tcgtctcttc cgggttgtca tgctcggtat acaccacgcc ggagcagcag taaccaaaca
    36481 gcggcgcacg ccagttacgc tggttgttta tttcgttgaa ggtcatccag tatttgactt
    36541 tatgcttgta gcgttcgaat accacttccg cgaaacggac aaagaaatca acgaatttac
    36601 ggttggtcca gctaccgtat tgctgcacca gatgcagcgg catttcaaag tgggagaggg
    36661 ttatcaccgg ttcgatgttg tatttcagca gttcatcgaa cagagagtcg taaaacttca
    36721 gcccttcttc gtttggctga gcttcatcac cttttggaaa aatacgcgtc caggcgatgg
    36781 aagtccgaaa acatttgaag cccatttcgg caaatagctt gatgtcttcc ttgtagtgac
    36841 cataaaaatc aacggcttca tggtttggat agtattttcc tggcaagact tctttggtga
    36901 tttcgcgcgg cacgccgtgt gcgccaccgg tcagaacgtc acaaatgctc ggtcctttgc
    36961 cgcctttgtt ccagccgcct tcgacctgat gagcggcaac ggcgccaccc cataagaaat
    37021 ctttcggtaa ggtgagtttt ttcactatca taggctccag atcagtcaat tcttacagga
    37081 aatagtacca tgcagccaaa cgacatcact ttttttcaac gtttccagga tgacattctg
    37141 gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa aacgggtgat
    37201 gtgcttcgtg tcggacgttt tgaagatgac ggttattttt gcacgattga agtcaccgca
    37261 acctcaaccg taacgctgga tacactgacg gaaaaacatg cagagcagga aaatatgacc
    37321 ctgcctgaac tgaaaaaggt cattgctgac atctatcccg gtcagacaca attttatatg
    37381 attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt cgcatttaat
    37441 ttcatgattt ttaggagatt agtctcaaat cagaatttat tttagctaac aggtgttcac
    37501 tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt ctctggagta
    37561 agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg aaattgccaa
    37621 cagcgtcagt cacggcattg ggctggtgtt tggtatcgtt gggctggtgt tgctactggt
    37681 tcaggcggtg gatctcaatg ccagcgccac agcgataacc agttacagcc tctatggcgg
    37741 cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc atcaacgggc
    37801 aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctgttgattg ccggaaccta
    37861 cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga tgattgttat
    37921 ctggagccta gcattgctgg ggattctgtt caaactgacc atcgcgcacc gattcaaaat
    37981 tttatctctg gtgacttatc tggcgatggg ctggctgtcg ctggtggtaa tttatgaaat
    38041 ggcagttaag ctcgcggcgg gcagcgttac cttactggcg gtaggcggcg tggtttattc
    38101 gctcggggtg attttctacg tctgcaaacg cattccatac aaccatgcca tctggcacgg
    38161 cttcgtgctc ggcggtagtg tgtgccactt tctggcgatc tatttgtata ttgggcaggc
    38221 gtaatgggta tgcctttgta ggcctgataa gacgcgtcag cgtcgcatca ggcatcggag
    38281 cgcaaccgcc ggatgcggcg tgaacgcctt atccgatcta cgggcctcgt ccctgttgtg
    38341 ataaaggcat caaatcccag acatgataag acgcgtcagc gtcgcatcag gcgataaatg
    38401 cctgatgcga tacagagatt tactcttcga gcgaatacgg cagcggctcg atatgcagtg
    38461 tattcgcatc gtcgcgtacg cggaagatgc tgtccggttc catatcgtta ttcatgacga
    38521 cctgtaccac gacctgacca tcttccagtt ttaccgcagc cagcaccgta ccggtacgac
    38581 gccagttctc gcccattttc agctctaagt cttcaccagc ttccggcagt cggctggcac
    38641 tacctgccag caaccagagc gcgcgtttgt tggcaccacg gaattttgct cgcgccacca
    38701 tctcttgtcc ggtataacag cctttcttaa agctgatacc gcccagcgcc tggagattgg
    38761 tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat caccgggaaa ccagcttcaa
    38821 tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc agtttatcgg
    38881 tcagcatatt agcagtagct tcatcggtta cgatcaggaa acgttctgcc gggtgttcaa
    38941 accatagcaa agtggtcgcg ccttctttga ctacctgttt ttctttcgaa ggcagttcgc
    39001 taaagagatt tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc agcacacgct
    39061 cgtcgtctgg cgcgatggtc actttagaga ataccgcata ttttttcagt tcagtcagct
    39121 gcggttcacg cacgctgcgc cgttcaatcc atgcaaagcc atcgccgtcg cggaacagac
    39181 gtaaattgct ccacatttta cctttggcgt cgcaatgggc ggcgagcagg tgctgatctt
    39241 ctgccatctg gctgacatct gctgtcacct gaccctgcat atatttttcg ctgtccgcgc
    39301 cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagcggt aaacgggcag
    39361 aagccgtagg ctgacgtgga ggaaaaggtg taaaagccat aatagagtcc tgagttgctt
    39421 aacggcgtat taatgactaa tggtaaaaga gctatgaggc aatgcaagtg ctttcactac
    39481 gcgatctgtg ccttctgcga ggcgactgcc aaaaaaatga agcccttgaa ttgttgtgaa
    39541 ttaatttcgc gatcgcgatg gtgtttactg atattcccgt aaaagaagtc gaaaaacacg
    39601 ttacaatagc cgggtactgg attttcgtga gacaggaaga agatggacat taacaacaaa
    39661 gcccgcattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc aatcatgccg
    39721 tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt tattcgtctg
    39781 ctggaatgtg acgatccgga cctgtttaac tggctgatga atcacggtaa accggccgat
    39841 gcagaactgg aaataatggt ccgactcatc cagacacgga accgggaacg tggtcctgtg
    39901 gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga ttcatgggct
    39961 ggttgccgct gttattttac tcatgccctg gccgctcagt tacaccccgt tatggatggt
    40021 gttactttcg ctggtggtat ttgattgcgt tcgcagccag cggtgtatta atgctcgcca
    40081 gggggaaatt cgcttgttga tggacgggcg tttgcgttgg caagggcagg agtggagcat
    40141 cgtcaaagca ccgtggatga ttaagagcgg catgatgctg cgtttacgtt ctgatggcgg
    40201 taaacggcaa catttatggc tggcagccga cagcatggac gaagccgaat ggcgggattt
    40261 acggcggatt ttgttgcaac aagagacgca aagataaaga tgacggcaaa gcatatgcgc
    40321 taagccgtca gtgagatcag gcgtaatgct ctgccatttc gttgaggatt tgctcgcacc
    40381 agctctgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc gccagaccca
    40441 cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat ccttccgttg
    40501 gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc ataccgagcg
    40561 catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc gcaataattt
    40621 taccttcaag gttcaggtcg tcgagctgat cccagacggc ttcccagtct tcttggattt
    40681 caccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc attaatttcg
    40741 gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata tcgcggattt
    40801 tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc atattcataa
    40861 cgtaagcttc tcgattctgc tgtggctttt gcagctagtg tatcagaatc gctttagtca
    40921 cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag tgaaacagga
    40981 tctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc tggctgaaaa
    41041 tacgttgaac gcttaccgtc gcgatctgtt aatgatggtg gagtggttgc atcaccgcgg
    41101 gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag aacggctgga
    41161 gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc gattgttcca
    41221 gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt gcgcatctcg cttcaccgaa
    41281 attgccccag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt tattacaggc
    41341 gccattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag tgttgtatgc
    41401 taccgggctg cgtgtctctg aactggtcgg gctgacaatg agtgatatca gcctgcgtca
    41461 gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc ctggtgccgt taggggaaga
    41521 ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgc tgaatggtgt
    41581 gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac agaccttctg
    41641 gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc tgtcaccgca
    41701 tgtgttgcgt cacgcttttg ccactcattt attaaatcat ggtgcggatt tacgcgtggt
    41761 gcagatgcta ctgggccaca gcgatctttc caccacgcaa atttatacgc atgtcgctac
    41821 cgagcgtctg cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc tgaaaagaac
    41881 gggaagattt atgaagaaag gttttatgtt gtttactttg ttagcggcgt tttcaggctt
    41941 tgctcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca tcaaaagcag
    42001 cgatattcag cccgcgcccg tagctggcat gaagacagtt ctgactaaca gcggtgtgtt
    42061 gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg ttagtggcac
    42121 ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc ttgaaaaaga
    42181 gatgatcgtt tataaagcgc cgcaggaaaa acacgtcatc accgtgttta ctgatattac
    42241 ctgtggttac tgccacaaac tgcatgagca aatggcagac tataacgcgc tggggatcac
    42301 cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga aagaaatgaa
    42361 agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg caggtaaaag
    42421 cgtcgcacca gccagctgcg acgtggatat tgccgaccat tacgcacttg gcgtccagct
    42481 tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttc cgggttacca
    42541 gccgccgaaa gacatgaaag aattcctcga cgaacaccaa aaaatgacca gcggtaaata
    42601 attcgcgtga aacaacagat acaacttcgt cgccgtgaag tcgatgaaac ggcagacttg
    42661 cccgctgaat tgcctccctt gctgcgccgt ttatacgcca gccggggagt acgcagtgcg
    42721 caagaactgg aacgcagtgt taaaggtatg ttgccctggc agcaactaag cggtgtcgaa
    42781 aaggccgttg agatccttta caacgctttt cgcgaaggaa cgcggattat tgtggtcggt
    42841 gatttcgacg ccgacggcgc gaccagcacg gctctaagcg tgctggcgat gcgctcgctt
    42901 ggttgcagca atatcgacta cctggtacca aaccgtttcg aagacggtta cggcttaagc
    42961 ccggaagtgg tcgatcaggc ccatgcccgt ggcgcgcagt taattgtcac ggtggataac
    43021 ggtatttcct cccatgcggg agttgagcac gctcgctcgt tgggcatccc ggttattgtt
    43081 accgatcacc atttgccggg cgacacatta cccgcagcgg aagcgatcat taaccctaac
    43141 ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc gttttatctg
    43201 atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg cggcatcgca
    43261 attcctaacc tggcagaact gctggatctg gtagcgctgg ggacagtagc ggacgtcgtg
    43321 ccgctggacg ctaataatcg cattctgacc tggcagggga tgagtcgcat ccgtgccgga
    43381 aagtgccgtc cagggattaa agcgctgctg gaagtggcaa atcgcgatgc acaaaaacta
    43441 gccgccagcg atttaggttt tgcgctggga ccgcgtctca atgctgccgg gcgactggat
    43501 gatatgtccg ttggtgtggc gctgttgctg tgcgacaaca tcggagaagc gcgcgtgctg
    43561 gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg aatgcaggtt
    43621 gaagccctga ccctgtgcga gaaactggag cgcagccgtg acacgctacc cggcgggctg
    43681 gcaatgtatc accccgaatg gcatcagggc gttgtcggta ttctggcttc gcgtatcaaa
    43741 gagcgttttc accgtccggt tatcgccttt gcgccagcag gtgacggtac gctgaaaggt
    43801 tccggtcgct ccattcaggg gctgcatatg cgtgatgcgc tggagcgatt agacacactc
    43861 taccccggca tgatgctcaa gtttggcggt catgcgatgg cggcgggttt gtcgctggaa
    43921 gaggataaat tcgaactctt tcaacaacgg tttggcgaac tggttactga gtggctggcc
    43981 ccttcgctat tgcaaggcga agtggtgtca gacggtccgt taagcccggc cgaaatgacc
    44041 atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt cccggagccg
    44101 ctgtttgacg gtcatttccg tctgctgcaa cagcggctgg tgggcgaacg tcatttgaag
    44161 gtgatggtcg aaccggtcgg cggcggtcca ctgctggatg gtattgcttt taatgtcgat
    44221 accgctctct ggccggataa cggcgtgcgc gaagtgcaac tggcttataa gctcgatatc
    44281 aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg gccaatttag
    44341 cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt atcaccgcat
    44401 tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt aaataatcgc
    44461 attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact acgacgccaa
    44521 gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct ggaacgaacc
    44581 cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg tcgacaccct
    44641 cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg ctgtagaagc
    44701 tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg aagaaaaact
    44761 ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg actgttacct
    44821 cgatattcag gcggggtctg gcggtacgga agcacaggac tgggcgagca tgcttgagcg
    44881 tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact gaaatcatcg aagagtcgga
    44941 aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt acgcttacgg
    45001 ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt aaaagcccgt ttgactccgg
    45061 cggtcgtcgc cacacgtcgt tcagctctgc gtttgtttat ccggaagttg atgatgatat
    45121 tgatatcgaa atcaacccgg cggatctgcg cattgacgtt tatcgcgcgt ccggcgcggg
    45181 cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt acccacatcc cgaccgggat
    45241 cgtgacccag tgccagaacg accgttccca gcacaagaac aaagatcagg ccatgaagca
    45301 gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga aacaggcgat
    45361 ggaagataac aaatctgaca tcggctgggg cagccagatt cgttcttatg tccttgatga
    45421 ctcccgcatt aaagatctgc gcaccggggt agaaacccgc aacacgcagg ccgtgctgga
    45481 cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag gaaccaacat
    45541 gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg aactgaaaac
    45601 gcgtcgtgag aagctggcga acctgcgtga gcagggaatt gccttcccga acgatttccg
    45661 tcgcgatcat acctctgacc aattgcacgc agaattcgac ggtaaagaga acgaagaact
    45721 ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg atgacccgcc gtattatggg
    45781 taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc attcagctgt acgttgcccg
    45841 tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc tcggcgacat
    45901 cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta tccactgtac
    45961 cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc acggcttgca
    46021 ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg atgaatcccg
    46081 taacaccttt aaagtacgct cgcagatcct ctctggtatt cgccagttca tggtgaaccg
    46141 tggctttatg gaagttgaaa cgccgatgat gcaggtgatc ccaggcggtg ccgctgcgcg
    46201 tccgtttatc acccaccata acgcgctgga tctcgacatg tacctgcgta tcgcgccgga
    46261 actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa tcaaccgtaa
    46321 cttccgtaac gaaggtattt ccgtacgtca taacccagag ttcaccatga tggaactcta
    46381 catggcttac gcagattaca aagatctgat cgagctgacc gaatcgctgt tccgtactct
    46441 ggcacaggat attctcggta agacggaagt gacctacggc gacgtgacgc tggacttcgg
    46501 taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc cggaaaccga
    46561 catggcggat ctggataact tcgactctgc gaaagcgatt gctgaatcta tcggtatcca
    46621 cgttgagaag agctggggtc tgggccgtat cgttaccgag atcttcgaag aagtggcaga
    46681 agcacatctg attcagccga ccttcattac tgaatatccg gcagaagttt ctccgctggc
    46741 gcgtcgtaac gacattaacc cggaaatcac agaccgcttt gagttcttca tcggtggtcg
    46801 tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa gatcaggcgc aacgcttcct
    46861 ggatcaggtt gccgcgaaag acgcaggtga cgacgaagcg atgttctacg atgaagatta
    46921 cgttaccgca ctggaacatg gcttaccgcc gacagcaggt ctggggattg gcatcgaccg
    46981 tatggtaatg ctgttcacca acagccatac catccgcgac gttattctgt tcccggcgat
    47041 gcgtccggtg aaataagcat tacgttatgc tcacaacccc ggcaaatgtc ggggtttttt
    47101 atttaagctg ggtaaatgca gataatagta ttctggcttc gcgatttgtc gcctgcatca
    47161 ccatccacgg actgaacgcc cacggcgtgg catcaatacc gcgtaataca tcagctaaat
    47221 cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg gtggtgcgtg
    47281 cggcaaatac cggacacact tcattttcca caatgccact cggatcggtg gcgcggtagc
    47341 gaaagtcagg atagatagat tcaggaggcg taatttccac gccaagctca taacggcaac
    47401 ggcggatcac cgcgtcttcg ctgctttctc ccagttgtgg gtgcccacaa accgagttag
    47461 tccacacgcc aggccacgct tttttgctca gtgcgcggcg ggtaactaat aattgcccct
    47521 tggcattaaa cagccaactg gagaacgcga gatgtaagag ggtgtctgcc gtgtgtgcgg
    47581 catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg acgtgttccg
    47641 tttgcataat ttctcacatg taattctgat cactcatcat tatagataat tgaagagtga
    47701 aatgattaac caagagagcg tttatcgaca accgcccgtt ttgagcggat gccgacaggt
    47761 aaaaaccatt taaaaaagta gcatcatata tttgaagcga ctttcaatgg ccattgcaac
    47821 acagtcagaa atgacaggaa tagagagagt aaaaagcaac aacagactga atatagcgcg
    47881 cattaaaaaa ttcatattat tctccatgct catttttctt cagcttgcgc atcagaagcc
    47941 acatacgtac tgtcctgact tttaaatctt tatcagagac ggattccttc accaggtcag
    48001 catgatgctg atagccatta aagaagacgt tcagcacaac ggcgctaagg gttgcgagca
    48061 taatgccgct atgtagcagc ggttgcagta cggccggtaa cttagaaaag aaatcgtgag
    48121 agagcgtcgg agtcatgcca acgccgagac tgatcgccac aatatagagg ttgtaacggt
    48181 tggtggtgta gttacagcgc gacagaattc gaatccctgt cgccagtacc atgccgaaca
    48241 tcactagacc agcgccgccc agcacaaatt gcggaatgga ggctaccagc accgccattt
    48301 ttggcaccat gccaaacagg attaatataa ttcccgaaga aatacacacc cagcggctat
    48361 gaacgcgcat cacgctaacc aggccaacgt tttgagaaaa tgacgtgtgg gggaagctgt
    48421 taaacgtgcc gcctatcatt gtccctacgc catcgacacg cagcccgcga ataatatcgt
    48481 gtgaagagag tttacgaccg actatttcac ccagtgccag gaacatcccc atcgactcga
    48541 taaacacgat gattaacacg gcagtcatgg tcagaatgga aacgggatcg aaaatcggca
    48601 taccaaacga catcggggtg acaatcgcaa accacgaggc atcatgtagc ccggataaat
    48661 tgacttcatt catcatccac gaaagtaaaa agccaaatac aatccccagt aatacggcga
    48721 cgttggacat aaatcctttc gcatagcgag taatgagcaa gataaaaatt aagacggcaa
    48781 aggagatacc taaataaacg ggattaccat attgcggatt ccctttacct ccagcggccc
    48841 agtcaatacc cacctgaatg atgctaagcc caatagaagt aataaccaca ccggtaacca
    48901 gtggcgggaa taaaggcatc aagcgaccaa taagtggcgc taataatgtg gtgataaaac
    48961 ccgcggcgat agtggcacca aatatcccca gcaggccgat atccgggttc atcccaatgg
    49021 ctatcattgg tgttacagca gcaaaggtca ccgacataat caccggcagg cggatcccca
    49081 taaagcggcc gataccgata cattgcaata atgtgacgat cccgcagcaa aagagatccg
    49141 agctaatgag catcgcaata gcatcttttg agaggcccag tcggtcacca atcataagag
    49201 gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt atgatcagct
    49261 ttcctggtga taatatgcgg tcaacctcat cagttgggcg gtcttgccca gaagatgagg
    49321 gaagttggga atctatggcg ctcatcagtg tttctccttt cgcccgccag aataaaaagc
    49381 aaccgtttac gttaatgaaa ctgtctaaac acatgctcgt ggagtgccag tttttgcgag
    49441 gaaggggagt tagagacagg aattgcggag gcaatcacat aatcgtgctt aaaccacgaa
    49501 aatgacaaga gaacctgata atggaattat ctgtaatttt ctcatattga tatttcatta
    49561 atcattctgg c
//
LOCUS       DAJTYG010000019        49132 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTYG010000019 DAJTYG010000000
VERSION     DAJTYG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49132
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.019"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(12..323)
                     /locus_tag="OO568_001192"
     CDS             complement(12..323)
                     /locus_tag="OO568_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR6640626.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            486..582
                     /gene="ffs"
                     /locus_tag="OO568_001193"
     ncRNA           486..582
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO568_001193"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            702..1055
                     /locus_tag="OO568_001194"
     CDS             702..1055
                     /locus_tag="OO568_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6640627.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="OO568_001195"
     CDS             complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="OO568_001195"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR6640628.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FINVIPYSSWQIDAAIIGNAHNVVITSSDKIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2811..3281)
                     /locus_tag="OO568_001196"
     CDS             complement(2811..3281)
                     /locus_tag="OO568_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6640629.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3397..3948)
                     /gene="maa"
                     /locus_tag="OO568_001197"
     CDS             complement(3397..3948)
                     /gene="maa"
                     /locus_tag="OO568_001197"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR6640630.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4120..4338)
                     /gene="hha"
                     /locus_tag="OO568_001198"
     CDS             complement(4120..4338)
                     /gene="hha"
                     /locus_tag="OO568_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6640631.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="OO568_001199"
     CDS             complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="OO568_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6640632.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5284..8433)
                     /locus_tag="OO568_001200"
     CDS             complement(5284..8433)
                     /locus_tag="OO568_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6640633.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAVSLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="OO568_001201"
     CDS             complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="OO568_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6640634.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9791..10438
                     /gene="acrR"
                     /locus_tag="OO568_001202"
     CDS             9791..10438
                     /gene="acrR"
                     /locus_tag="OO568_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6640635.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10566..13928
                     /gene="mscK"
                     /locus_tag="OO568_001203"
     CDS             10566..13928
                     /gene="mscK"
                     /locus_tag="OO568_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6640636.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQVQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="OO568_001204"
     CDS             complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="OO568_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6640637.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14315..14842)
                     /gene="priC"
                     /locus_tag="OO568_001205"
     CDS             complement(14315..14842)
                     /gene="priC"
                     /locus_tag="OO568_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6640638.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14912..15289
                     /locus_tag="OO568_001206"
     CDS             14912..15289
                     /locus_tag="OO568_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6640639.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15442..15993
                     /gene="apt"
                     /locus_tag="OO568_001207"
     CDS             15442..15993
                     /gene="apt"
                     /locus_tag="OO568_001207"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6640640.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16122..18047
                     /gene="dnaX"
                     /locus_tag="OO568_001208"
     CDS             16122..18047
                     /gene="dnaX"
                     /locus_tag="OO568_001208"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6640641.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWTAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17380..17444
                     /gene="dnaX"
                     /locus_tag="OO568_001208"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18100..18429
                     /locus_tag="OO568_001209"
     CDS             18100..18429
                     /locus_tag="OO568_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6640642.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18429..19034
                     /gene="recR"
                     /locus_tag="OO568_001210"
     CDS             18429..19034
                     /gene="recR"
                     /locus_tag="OO568_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6640643.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19144..21018
                     /gene="htpG"
                     /locus_tag="OO568_001211"
     CDS             19144..21018
                     /gene="htpG"
                     /locus_tag="OO568_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6640644.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21199..21843
                     /gene="adk"
                     /locus_tag="OO568_001212"
     CDS             21199..21843
                     /gene="adk"
                     /locus_tag="OO568_001212"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6640645.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            21975..22937
                     /gene="hemH"
                     /locus_tag="OO568_001213"
     CDS             21975..22937
                     /gene="hemH"
                     /locus_tag="OO568_001213"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6640646.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22934..23894)
                     /gene="aes"
                     /locus_tag="OO568_001214"
                     /pseudo
     CDS             complement(22934..23894)
                     /gene="aes"
                     /locus_tag="OO568_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24046..25350
                     /gene="gsk"
                     /locus_tag="OO568_001215"
     CDS             24046..25350
                     /gene="gsk"
                     /locus_tag="OO568_001215"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6640647.1"
                     /translation="MKFPGKRKSKNYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(25483..27159)
                     /locus_tag="OO568_001216"
     CDS             complement(25483..27159)
                     /locus_tag="OO568_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6640648.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27397..28617)
                     /gene="fsr"
                     /locus_tag="OO568_001217"
     CDS             complement(27397..28617)
                     /gene="fsr"
                     /locus_tag="OO568_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6640649.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALPGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMISGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28835..30487
                     /gene="ushA"
                     /locus_tag="OO568_001218"
     CDS             28835..30487
                     /gene="ushA"
                     /locus_tag="OO568_001218"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6640650.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSIGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30524..31003)
                     /gene="ybaK"
                     /locus_tag="OO568_001219"
     CDS             complement(30524..31003)
                     /gene="ybaK"
                     /locus_tag="OO568_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6640651.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31207..32001)
                     /locus_tag="OO568_001220"
     CDS             complement(31207..32001)
                     /locus_tag="OO568_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6640652.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALLVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32139..32480
                     /locus_tag="OO568_001221"
     CDS             32139..32480
                     /locus_tag="OO568_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6640653.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32695..35199)
                     /gene="copA"
                     /locus_tag="OO568_001222"
     CDS             complement(32695..35199)
                     /gene="copA"
                     /locus_tag="OO568_001222"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6640654.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35461..36393
                     /gene="glsA"
                     /locus_tag="OO568_001223"
     CDS             35461..36393
                     /gene="glsA"
                     /locus_tag="OO568_001223"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6640655.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36396..37688
                     /locus_tag="OO568_001224"
     CDS             36396..37688
                     /locus_tag="OO568_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6640656.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37813..38220
                     /gene="cueR"
                     /locus_tag="OO568_001225"
     CDS             37813..38220
                     /gene="cueR"
                     /locus_tag="OO568_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6640657.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLMTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38221..38679)
                     /locus_tag="OO568_001226"
     CDS             complement(38221..38679)
                     /locus_tag="OO568_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6640658.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTYVEVIAIEGITLHIRAVSS"
     gene            complement(38676..39593)
                     /locus_tag="OO568_001227"
     CDS             complement(38676..39593)
                     /locus_tag="OO568_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6640659.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39739..40416
                     /gene="fetA"
                     /locus_tag="OO568_001228"
     CDS             39739..40416
                     /gene="fetA"
                     /locus_tag="OO568_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6640660.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EVRYELA"
     gene            40403..41182
                     /gene="fetB"
                     /locus_tag="OO568_001229"
     CDS             40403..41182
                     /gene="fetB"
                     /locus_tag="OO568_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6640661.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41245..42099)
                     /gene="cnoX"
                     /locus_tag="OO568_001230"
     CDS             complement(41245..42099)
                     /gene="cnoX"
                     /locus_tag="OO568_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6640662.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42160..42969)
                     /gene="ybbO"
                     /locus_tag="OO568_001231"
     CDS             complement(42160..42969)
                     /gene="ybbO"
                     /locus_tag="OO568_001231"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6640663.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42983..43581)
                     /gene="tesA"
                     /locus_tag="OO568_001232"
                     /pseudo
     CDS             complement(42983..43581)
                     /gene="tesA"
                     /locus_tag="OO568_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43552..44238
                     /locus_tag="OO568_001233"
     CDS             43552..44238
                     /locus_tag="OO568_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6640664.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44235..46649
                     /locus_tag="OO568_001234"
     CDS             44235..46649
                     /locus_tag="OO568_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6640665.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="OO568_001235"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="OO568_001235"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6640666.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQATIAVVEDPFEIRLERLNEEYFFRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..>49132)
                     /gene="allS"
                     /locus_tag="OO568_001236"
     CDS             complement(48524..>49132)
                     /gene="allS"
                     /locus_tag="OO568_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR6640667.1"
                     /translation="AVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVT
                     GTEALANTFSLDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTK
                     RVAWRLPGQKEIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPP
                     SPLSLAWRKFGSGKAVEDIVTLFTQHRPEISGFLEIFGNPRS"
BASE COUNT        11872 a        12759 c        12898 g        11603 t
ORIGIN      
        1 cccggagagg gttagtagtt ccagcgataa cgctgcaagt cgatttgccc gcttcccgat
       61 accatcacac cttctgccag taatgcctgt cgctgacgct gtaaatccgg cccggttagc
      121 gaaattgtgc catggcgatt aaccacccgg tgccagggta aggtgctgcc ttcagggaga
      181 cgctttaaca caccgcccac ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca
      241 tcaccgtaag tggtgacata gccttcggga atagcggcga cgatttgcca gacgcgttgg
      301 ggaaatgaat cttctttttc catcttttct tcctgaggta atttttcagc ataatctgga
      361 aaaacgcccg agtgaagtcg cattgcgcaa gaaaccagca tctggcacgc aatgggttgc
      421 aattagccgg ggcagcagtg ataatgcgcc tgcgcgttgg ttttcaacgc tctcaatggg
      481 ggctctgttg gttctcccgc aacgctactc tgtttaccag gtcaggtccg gaaggaagca
      541 gccaaggcag atgacgcgtg tgccgggatg tagctggcag ggcccccacc catttctgcc
      601 tcccaccgtt tcgtcaaaaa atcccagcat ggctaaactt taaccacaac tgacgtcgca
      661 agaattgtct ggctgcgcag tacgcttcgg aggtatgtct gatgaagtat gttgatggtt
      721 ttgtggttgc cgttcctgcc gataaaaagg atgcctatcg ggaaatggcc gctaaggccg
      781 cgccattgtt taaagagttt ggcgcgcttc gtattgtcga atgctgggcc agcgatgtac
      841 cggatggcaa agtgaccgat tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg
      901 tctttagctg gattgaatac ccttcaaaag aggtccgcga cgcagctaat caaaagatga
      961 tgtcggaccc acggatgaaa gagttcggcg agtccatgcc gtttgacggc aagcgaatga
     1021 tctatggcgg attcgagtca atcatcgacg aataggtagc gtgacgggct gtgcggcgtg
     1081 ccgtcagccc aaaaattcac aaatgttgct cggcccagcg taagaaatct tctttcggta
     1141 atgccttgct gtagagccag ccctgaccgt agtgcacgcc atgctggcgt agccactctt
     1201 cttgtttact ggtttcgatt ccctccgcta ccattttcag tttcagtgtt tttgccattt
     1261 cgatgatatg cggcgtgaca tttttatatt ccagcgcatc aacgaaagat ttatcgatct
     1321 tcagaatgtc gacatccaga tcctgtaaat aacttaaact tgaatacccc gtaccaaaat
     1381 catcaagata aatttcatgg cccgcctccc ggtagcgaga aattatcggg gcgctggttt
     1441 tcggatcggc aaactcgcgt tcagtgagtt caagcgcgat ctgtctggga ttaacctgat
     1501 agtgattgat catttcacgc agcaattgcg ggattttttc cgaggtgagc acggtggatt
     1561 caagattgat cgaaatatgc tgctgtggat gctgacgcaa gcagtcgccc atatcttcaa
     1621 agacgcttct tataatcagt agcgtcaatg gctcagaaag gcccgtttgc tgtgccagcg
     1681 gaataaaact atctggtgac aaccaactac cgtctgtctg cggccagcgc gccagtgcct
     1741 cagcaccgac aattttgcca ttggctaagg agacaatcgg ctgatagtgc acgcaaatat
     1801 cacgattttc gatagcatcc tgcagccgat gatgcggtga ctggatacgg cgcaaaatac
     1861 gcagcacaaa catcgctgcc agcaggccaa tcaccaaccc gagcggtaac caaataaaga
     1921 cttgccgatg ccagcctttc tgcaacattt tcgttgaagc ccaggtgatg atcgaaatat
     1981 tcatctccgg gaagggcagg atatcgtaaa tgatcccatt attttcgata tgctcaccgg
     2041 gtgttttttg tagcctggta ataattccct gagcaatttt atcgctgctg gttatgacaa
     2101 cgttatgggc attgccaata atggcggcat caatttgcca tgagctatag ggaatgacat
     2161 taataaagga agcggggtcg atcatgacga cataatgtgc cgttcccatg gcgaccatgt
     2221 aacggataat gcctaaatcg ttatgcgatg ttaaccagac acgataacca tctttcgaaa
     2281 ttttacctgg ctcgggaaag gtatcgggcg gactttcatg ctccagagac gaacactggg
     2341 gaacgttgtt atcgatataa accacttcct gaatatagcg ataactgtaa gatacccgac
     2401 gcatttccat gagatgggct tcgctacagg cagcgccttg ccatctttcc agctcctgca
     2461 gcgcatcttt cccttgtgtc gccaccttat tggctcgaat agcgacgcgg gaggaatagg
     2521 tatccagctc ttcaataaac gatgtttcta cctgctgatg ggccagccag atgcttaagc
     2581 cgacaggcag caatactgaa agaatcagta ctcccgaaat aaggccgacc agatgtcgtg
     2641 ttctcaccag catatcctta ttaacacagt gtagggaaag tatatctgat tgtggaaaaa
     2701 ggctggcagg agataaggag gtagagctgg tatttcatga agtgatgggt ggaaacgatc
     2761 attgtcattt gaccaggcgc agagctttac gcctggtatg taaccctaac ttaagatgtt
     2821 gattcggcgc gagcgagggt aaaaatatcg taaaaagaca gttcaccttt acggacgatc
     2881 attttttgca gttgttcctt atgaacatcc gcgacacgcg gagagttcaa gatggcttca
     2941 accagcgcgg caggtacaaa gcgcgtgttg taccattcgc cgttatagca aacgcggaaa
     3001 tccagcacgt cgatatcttc atagcggtag cgtgggtaga catcgaaaaa gaagaaacca
     3061 ttaagcagga taaataatac aaccagtagc cagacagagc ctgacagcgt ttcggactgc
     3121 aacatcaccg ccagggtggc aaaaaaagca acgtacatac cgataaacag ccccggatgt
     3181 ttacggataa aactgatact aaagcggggt ttgttgtcgc gtttttcgcg ggtattcaga
     3241 gactcaatcg tttcggtcag caggcgttgt atttcggtca tcgtttgcct tcgtggtatc
     3301 tgcaactttc cagggaacac cctattttaa cgtgggaata gtagagggga agtaacagat
     3361 ctaccacaat tttgcataac agttgcgaaa aaccgattac aattttttaa ttattctggc
     3421 tggattaccg cccacgacaa cgttgtccgg gacacctttt gtgacaactg cacctgaagc
     3481 taccacgacg ttatcaccaa tggtcacacc agggttaatg accgcgcgtc cgccaatcca
     3541 gacgttatta ccgatggtga cgggtttccc cagttcagca ccgctattac gtgctacagg
     3601 gtcgatggga tgtgttgccg tgtagatatg aacgcctggt gccaacatac agttatcacc
     3661 gatgcgaata gggcagacat caagcatcac gcaatcgaag ttggcgaaaa aattattgcc
     3721 gagaaaaatg ttatagccat aatcacagcg aaacgttggc tcaatataag cctctgtcac
     3781 ctgaccgaat aaatcagcga gaatttgctg gcgtaatgtg tgctcttccg ctagggaatg
     3841 attgtatcgg tgaataagct gacgagcgcg caggcgatcg cgagataacg tctcatctgc
     3901 cgagcgatac aactcaccag caatcatctt ttctttttct gtgctcatta ctgacctccg
     3961 gttgctgaaa atgcggcaac acagtaagac gaaaagtcag tgaagagaac gttctcatta
     4021 catctgtgtg ataaagatca catagggtat gtcttcatgg cgaaaaagta taaaattctt
     4081 aataaacagc cggttatagc tccgaaagcg aattatttat tagcgaataa atttccatac
     4141 tgaggaaggg atcttgtcgt acagtttatt catggtcaat tcggcgaggc ggtgatctgc
     4201 ggctgagtaa aataccgcca gttcattatc tgataattcg tatttatttt tctcgataac
     4261 acgctccagc gtgtcaattg tctggcaacg acgtaaacgc attaaataat cggtttttgt
     4321 taaaggtttt tcggacatac ttctacccat ggttgtaata attctaacaa gataaactcg
     4381 caggattctc tttcgtcgca ttgacaaaac aacggaatag tcgattacct gacttccgcc
     4441 atttctgaag agcctgcata ttaataccat aactgctgaa caacataaag gtgtcatcca
     4501 gatattcgtc gatctgctca atgagcttat tgtcttcatt atacttaatt ttgtaattaa
     4561 gtgcgaaggt cgcaatatgc tcaatcagtt cattcaactg gaggttgatc gccgaggttg
     4621 ggtcgttaac ccagccatga ttgctttctt caaggtttgc aagacagtca tgatacaggg
     4681 tttcacagag aaacttaagc tgtgcgatat catgtctttt gggtgagtat tcatccataa
     4741 cgcgtcccct tcttagtggt tgaactaacg gacacctttc gggatggaaa aaacttactg
     4801 acctagactt gccctctcgt ttgttagctt aactataagc cactctttgc aggttttcat
     4861 cgcattatta cgaaacatta caattaggaa ggaatcggga caggactcac tttttcatac
     4921 tagcactaac ccgcagcagg taaaagcagt ttgctcgaac tgttcataac atttcaggat
     4981 tatcgccaga tgaaattggt gacactaata ccaggattgc tctgaatatg acgtaataac
     5041 cgaggaatga ataaagaatt accgcaaata attaagaata gcctctaaat gattatggat
     5101 tattaaatct attagcgcta cttaatataa attaactaac aatcagtatt ttttatgaat
     5161 tttatacgtg gttaatactg gttttcgtat gagatcctga gttggtggtt caattactcc
     5221 ttaatgttcg taggttatgc ataaaaaagg ccgcttacgc ggccttagtg attacatgtt
     5281 gtatcaatga tgatcgacag tatggctgtg ctcgatatct tcattcttgc ggctaaagcg
     5341 gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg ttgcggtcac
     5401 catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag caccagtact
     5461 gataaccagc ggcataacgc cgaggataaa cgccagcgag gtcatcagga tcggacgtaa
     5521 acgcatccgc accgcatcaa gcgtcgcttc aatcagacct ttaccttctt tatccatcaa
     5581 gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaacccaa tggttgtgag
     5641 cagtcctact tggaagtaaa cgtcattggt caggccacgg aaggtggcag ccagcaacgc
     5701 accgataacc cccagcggaa cgaccagcat aacggagaac ggagtcgacc agctctcgta
     5761 cagcgccgcc agacacagga atacgacaat caacgaaacc gcgtacagtg aaggtgcctg
     5821 gttgccggag agacgttcct gataggacat ccccgtccag tcatagccaa caccggtagg
     5881 cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt tacccggtgc
     5941 cgcctggcct aagatttcca tggatggcag gccgttgtaa cgttccagac gcggcgaacc
     6001 gtactcccaa cgagaagagg agaacgccga gaatggcacc atctgaccat cagcagcacg
     6061 aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg acatgacgta
     6121 aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc cccatgcagc
     6181 gcccagagtg gtgttaatgt cgttgataga aacacccagc gcctgcgctt tttcctggtc
     6241 gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc tggtcagcat
     6301 atcagggtgc ttcgctgctt ctgcaagcag ctggttacgc gcctgagtca gtttttcgtg
     6361 accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcagtac ccagttccac
     6421 gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga aagcgcgtgt
     6481 tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg cccagtcctt
     6541 caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga agccgttaac
     6601 ggcgaacacc gactcaacgt tgtttttttc tttggtcaga tagtaatgcg ttacctcatt
     6661 gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca tggtcataaa
     6721 tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca gataggccat
     6781 gccgaccacg atgatcagat acagcactag gtaacgcccc gtactgcgca gaataccgcc
     6841 tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttaaacc agccgaagaa
     6901 gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg tggcacaaag
     6961 agccggagtc aggatcaacg ctaccagtac cgacagcgcc attgctgaaa caatggtaat
     7021 agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg gtacgaatac
     7081 cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca tcgacttacg
     7141 ggtagcttct tttggcggca aaccttcttc cgccataaca cgctccacgt tttctaccac
     7201 aacgatagcg tcatccacca acaagccgat ggcgagcacc atcccgaaca ttgttagcgt
     7261 gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata ccaccggtac
     7321 ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcagg aacagataca taaccaggaa
     7381 cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga ttttcacgaa
     7441 cggcgtggtg tcgtacgggt aaacaatttt cagacccgac gggaagaacg gttccatctt
     7501 cgccagttca gcacggattg ccgcagcggt atccagcgcg tttgcaccgg tcgccagctt
     7561 gatccccaga ccggaagccg gttggccgtt aaactctgcg atgatgtcgt agttctcacc
     7621 acccagctca atcttcgcca catcacgcag cagtacgcgg gaaccatcct gattcacttt
     7681 cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa taatagaggc
     7741 gttaagctgt tggcctttca ccggcggcgt accaccgagc tgacccgccg caacctgggc
     7801 gttctgcgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt tgttcagctc
     7861 attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa catcacccac
     7921 gcccgacgta cggctgatgg catctttcat attcgccgct acgtagtcag agatatcctc
     7981 ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc tgctggatga
     8041 tttctcaacg ctcacccctt gctgctgaac ttcttgcggc agcaacggca tcgccagctg
     8101 cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact caaaggtcag
     8161 ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca ggttatcgat
     8221 accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt tcgcatcagc
     8281 gccggggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat attgcgccac
     8341 cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga tcacccacgc
     8401 aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt ttaagttaag
     8461 acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca gcggtaactt
     8521 cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt actacgcgat
     8581 cgcctgcttt cagaccttct gtcaccagcc acttatcgcc aatagcctgg cttgcaacga
     8641 tcggacgggt ttccactttg tcatccgcgc caactaccag tacggtggca tcaccacgcg
     8701 gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc ccttcttcca
     8761 gacgtgcgcg cacgaacata cccggcagca gagtgtgatc cgggttcggg aagatagcgc
     8821 gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc agcgtaccgt
     8881 cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg ccgttctctt
     8941 gtttcagcgt gccattcgcc agttcctgtt tcaggcgcag gaagtcgttg ctggactggg
     9001 tcacatcaac gtagatcgga tcaagttgct gcacggttgc cagcgcagtc gcctgaccgt
     9061 tctgtaccaa tgcgccttcc gtcacgttcg acttaccaat gcgaccgcta atcggagagg
     9121 tgactttggt gtaagccaga ttgatccgcg cagtttcaac ggcagctttc gccgcagtta
     9181 ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtacccttgc ttactgatgt
     9241 actgagtacc gagcagtttc tgataacgat tcaccgtcaa ttgcgcgata ttggctgcag
     9301 cctgggcttt cgccagatca cctttcgcac tgtcgtatgt cgcctgatag gtcgcaggat
     9361 caatctgata gagagagaca ccagcttcga tgtcgctacc ttctttgaaa ttacgcttca
     9421 ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg gtgcgacccg
     9481 gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca acggcgggca
     9541 tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct aagctgcctg
     9601 agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata tgtaaacctc
     9661 gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg ctgcgtttat
     9721 attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac gcctgtaaat
     9781 tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc aacacatcct
     9841 cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc tgggcgagat
     9901 tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag acaagtcgga
     9961 tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactag agcttgagta
    10021 tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa ttcatgttct
    10081 tgaatccacg gtgacagaag aacgacgtcg attattgatg gagattatat tccacaaatg
    10141 cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct gtctggaaag
    10201 ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt tgcctgcgga
    10261 tttaatgacg cgtcgggcag caattattat gcgcggctat atttccggcc tgatggaaaa
    10321 ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt acgtcgccat
    10381 cttactggag atgtatctcc tgtgccccac gcttcgtaat cctgctacta acgaataacc
    10441 ctgaatctga ctccaggatt tttcctagac attttcgtcg ttgctattct ggttcactgc
    10501 gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag aaaccttcat
    10561 tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt tcagcattta
    10621 ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcgcgggcg tcatcgaatg
    10681 gtgatctgcc gacaaaagcg gacctgcagg cgcaacttga ctcactaaat aaacaaaaag
    10741 atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta gccaccctcg
    10801 ataaaatcga tcgcataaaa gaagagacag ttcagctacg gcaaaaagtc gctgaagcgc
    10861 cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagcgatgtc gataacgacg
    10921 aagaaacgcg caaaattctg agtacgcttt cgttgcgcca gctggaaact cgcgttgccc
    10981 aggcgctgga cgatttgcaa aacgcacaaa acgatctggc gtcttataac agccagctgg
    11041 tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct tcgcagcagc
    11101 tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc ttacgtccca
    11161 gccagaaagt gttaatgcag gttcagcagg cgttgctgaa tgcggagatt gaccagcagc
    11221 gtaaaagcct ggaagggaac accgtcttgc aggatacctt gcaaaagcaa cgtgattacg
    11281 tgacggcgaa cagcgcccgt ctggagcacc agttgcagct gttgcaagaa gcggtaaaca
    11341 gcaagcgcct gactttaact gaaaaaacgg cgcaggaagc cgtctccccg gatgaagccg
    11401 cgcgtattca ggctaatccg ctggtgaaac aggaactgga aattaaccag cagttaagtc
    11461 agcgtctgat taccgcgact gaaaacggta atcagttgat gcagcaaaac attaaagtca
    11521 aaaactggct ggagcgggcg ctgcaatcgg aacgcaatat taaagagcag attgccgtcc
    11581 tgaagggcag cctgctgttg tctcgtatcc tttaccagca acaacaaacg ctgccctcgg
    11641 cggatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa cagtttgaag
    11701 ttaaccagca gcgtgatgca ctcttccaga gcgatgcgtt cgtcaacaaa ctggaagaag
    11761 gtcacaccaa cgaagtcaac agcgaagttc acgatgcgtt attgcaagtg gttgatatgc
    11821 gtcgcgaatt gctggatcaa ctcaacaaac agttgggtaa ccagctgatg atggccatta
    11881 acctgcaaat caaccagcag cagttaatga gcgtatcgaa aaacctgaaa tccatcctga
    11941 ctcagcaaat cttttgggtg aacagtaacc gtccaatgga ctgggactgg atcaaagcgt
    12001 tcccgcaaag cctgaaagat gaatttaagt cgatgaaaat cacggtgaac tgggaaaaag
    12061 cctggcctgc cgtttttatc gctttcctcg ccggtttgcc gctgctgttg attgccgggc
    12121 tgatccactg gcgtctgggc tggctgaaag cgtatcaaca gaaactggct tccgctgtgg
    12181 gttccctgcg taacgatagc cagctcaaca caccaaaagc gatccttatc gacctgatcc
    12241 gcgcgctgcc ggtgtgcctg attattctcg cggttggcct gattctgttg accatgcagc
    12301 tcaacatcag cgaactgcta tggtcgttca gtaaaaaact ggcgattttc tggctggtgt
    12361 ttggcctgtg ctggaaggta ctggagaaaa atggcgttgc cgtgcgtcac ttcggcatgc
    12421 cggagcagca gaccagccac tggcgtcggc aaattgtccg catcagtctc gcgttgctgc
    12481 ctatccattt ctggtctgtg gtggcagaac tttccccgct gcatctgatg gatgatgtgc
    12541 tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggta tggccaatgt
    12601 gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actagtcacc attaccgtgc
    12661 tgtcgattat cccgattgca ttgatggtgc tgactgccac gggttatttc tacaccacgc
    12721 tgcgcctctc cgggcgttgg attgaaaccg tttatctggt gatcatctgg aacttgctct
    12781 accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg cgtcgtgcgc
    12841 tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg ccggaagaac
    12901 ccaccattgc actggagcaa gttaaccagc agacgctgcg tattaccatg ttgctgatgt
    12961 ttgcgctgtt cggtgtcatg ttctgggcaa tttggtccga tttgatcact gtgttcagct
    13021 atctcgacag catcacgctc tggcattaca acggcactga agctggcgct gcggtggtga
    13081 aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg gcctgggcgt
    13141 tgattcgcaa cctgcctggt ttactggaag tgctggtgct ctcgcgactg aatatgcgcc
    13201 agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc gttggtgcga
    13261 tgacggtgtt cggatcgctg ggcgtctctt gggataaact ccagtggctg gccgcagcat
    13321 tatccgtagg tcttggtttt ggtttacaag aaattttcgg taacttcgtc tccggtttga
    13381 tcattctgtt cgaacgtccg gtgcgtattg gcgatacggt aaccattggt agcttctcgg
    13441 ggacggtaag taagatccgt attcgtgcga caacgattac cgatttcgat cgcaaagaag
    13501 tgatcatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg ttgactgaca
    13561 ctactacgcg tctggtgatc cgtctcggcg tggcctatgg ctccgatctg gaaaaagtgc
    13621 gtaaagtgtt actgaaggcg gcgactgagc acccaagggt gatgcacgaa ccaatgccgg
    13681 aagtcttctt tacggcattt ggtgccagca cgttggatca tgagctgcgt ctgtatgtgc
    13741 gtgaactgcg tgaccgtagc cgtactgtcg atgagctgaa ccgtactatc gatcagctgt
    13801 gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg cacaacgaga
    13861 agggcgatga ggtgacggaa gtaaaacgcg actacaaagg cgacgatccg acaccagcgg
    13921 ttgggtaata accaaagggg gcgacattta gttgccccga gattactgac aaagtgcgcg
    13981 ttgttcatgc cggatgcggc gtgaccgcct tatccggcct acgaaaccgc aagaattcaa
    14041 tatattgcag gagcggtgta ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgc
    14101 ccgcaatctt aggggacatt tagcggcccc atttatttct cacttttccg cctcatcatc
    14161 gtgcgttaat ttcttttcat aatcacgctt tacaatatcc agcgcgcgca gcacggtact
    14221 ggcagggatc tgattttcct ccagcagcac aatcaaatcg acagccagtt tgacatcgtc
    14281 aggggcattt tcaagtgaca tactctttcc attgctagcg ggttaagcgc gctagcctgt
    14341 tttcgatttt ttccagcgca tggcggcagc gtgccaggcg cgcttcatag gcttccactt
    14401 cacgatgcag cgtttgctgt tccacgagat cggtcacccg cgccaggcgg gctctgcgtt
    14461 cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt ttacgctgcc
    14521 agcgggcaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg gcggcttcac
    14581 gcgcgatggc ttccagttgt gccgccagat gttccgccag ccaggccact tgcggcagct
    14641 gttgctgctc aactgcatga cgaagcgcag ccagattatc gcccgcctcg tcgagacaag
    14701 cctgtagtgt tgtcgcacga gtctgaaaaa gatgcctgtc gaaacgagca cttagcgtgg
    14761 cgaactgtga caccggggca caacgctgac gcagcgtagc gagctgtcct tccagttttt
    14821 ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg ttatgctaaa
    14881 gtagccactc tgtaagctga caacactcaa tatgcaacga atcattttaa tcatcattgg
    14941 ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac cgacgacgcc
    15001 gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc acgcctggtt
    15061 gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc atgcgatgcc
    15121 gcgcggcgtc aaaccgcggg cgattttgct tattttgctc acgtttgcca tttctctgtg
    15181 gttcgtccag atgccatggg tgcgcatcat gttgctggta attctcgcct gtttgctttt
    15241 ctatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa gcacaacaat
    15301 cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca caggcgcagg
    15361 cggtcaaagg ttaaacaaca gttacttttg atacgtttaa aacgcgccgt gagtaccacc
    15421 gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta tctcaaaaat
    15481 agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga tgtcaccagc
    15541 ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga gcgttacaaa
    15601 aatgcgggca ttaccaaagt tgtcggcacc gaagcgcgtg gcttcttgtt tggcgctccg
    15661 gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa actgccgcgt
    15721 gaaaccatca gtgaaaatta cgacctggaa tacggcaccg atcagctgga aatccacgtt
    15781 gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc aaccggcggc
    15841 actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtggc tgacgctgcg
    15901 ttcattatca acctgttcga tctcggcggc gaacagcgtc tcgaaaaaca gggcattacc
    15961 agctacagcc ttgtcccgtt cccgggccat taattatcgc cagtcttgtg ctgcccacgc
    16021 tacggacagc acaagatgtg cattcagcct cgccgttctg acggggctgt gttagcatta
    16081 ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat caggttttag
    16141 cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat gtgctgaccg
    16201 cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt tccggcaccc
    16261 gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac tgcgaaaccg
    16321 gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag caggggcgct
    16381 ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagat acccgcgacc
    16441 tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat ctgatcgacg
    16501 aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaaccctt gaagagccgc
    16561 cggagcacgt taagtttctg ctggcgacga ccgatccaca gaaattgccg gtgacgattt
    16621 tgtcacgctg tctgcaattt catctcaagg cgctggatgt cgagcaaatt cgccatcagc
    16681 ttgagcacat cctcaatgaa gaacatatcg ctcacgagcc gcgggcgctg caattgttgg
    16741 cgcgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag gcgattgcca
    16801 gcggtgacgg ccaggtttca acccaggcgg tcagtgcgat gctgggtacg cttgacgacg
    16861 atcaggcgct gtcgctggtt gaagcgatgg tcgagggcaa cggcgagcgc gtaatggcac
    16921 tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg gaaatgctcg
    16981 gcttgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc aacgacatgg
    17041 ccgccatcga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg gatattcagc
    17101 tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg gaccgccgca
    17161 tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccgcgtatg ccgctgcctg
    17221 agccagaagt gccacgccag tcctttgcac ccgtcgcgcc aacggcagta atgacgccaa
    17281 ccctggtgcc gccgcaatca gcgccgcagc aggcaccgac tgtaccgctc ccggaaacca
    17341 ccagccaggt gctggcggcg cgccagcagt tgcagcgcgt gcagggagca accaaagcaa
    17401 aaaagagtga accggcagcc gctacccgcg cgcggccggt gaataacgct gcgctggaaa
    17461 gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg ctggaaaaag
    17521 cgccagccaa aaaagaagcg tatcgctgga aggcgaccac tccggtgatg cagcaaaaag
    17581 aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa acgccggaac
    17641 tggcggcgaa gctagcggca gaagccattg agcgcgaccc gtggacggca caggtgagcc
    17701 aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa gaggagagcg
    17761 acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac cgcggcgcac
    17821 agcaaaaact ggctgaagcg ttgagcatgt taaaaggttc aacggttgaa ctgactatcg
    17881 ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg atatacgaag
    17941 aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag accctgcgtc
    18001 ggtttttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg taagcacagc
    18061 ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa aggcggtctg
    18121 ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat gcaggaagag
    18181 atcgcgcagc tggaagttac cggcgaatct ggcgcaggtc tggtaaaagt gaccatcaac
    18241 ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga cgacaaagag
    18301 atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat tgaagaaacg
    18361 cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg ctttaagatg
    18421 ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg cgctgtctgc
    18481 cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag cgcgatcgta
    18541 gcggcgggat gcgtctggcg caggcgctca cccgggcgat gtcggaaatc ggccactgcg
    18601 ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat ccgcgtcgtc
    18661 aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catctacgcc attgagcaga
    18721 cggggcagtt ttctggtcgt tattttgtgt tgatggggca tctgtcaccg ctggacggca
    18781 tcggtccgga tgatatcggg cttgatcgtc tggaacagcg tctggcagag gaaaaaatca
    18841 ctgaagtgat cctcgccacc aatcccacgg ttgaaggtga agctaccgct aactacattg
    18901 ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc gtaccggttg
    18961 gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ctccctcgcc gggcgtcata
    19021 agattcgttt ttaagcaaac gagagcagga gcacctgctc tcgcttgaaa ttattctccc
    19081 ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt gaggtagacc
    19141 tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct tctgcacctg
    19201 atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat ctctaacgcc
    19261 tccgatgcgg cggacaagct gcgtttccgt gcgctatcta acccggacct gtacgaaggt
    19321 gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgcacgct gaccatctcc
    19381 gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac tatcgctaaa
    19441 tccggtacca aatcattcct cgaatccctg ggttctgacc aggcgaaaga cagccagctg
    19501 atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa agtgaccgtg
    19561 cgtactcgcg cggcaggcga aaaaccagaa aacggcgtct tctgggaatc ggctggcgaa
    19621 ggtgaatata ccgttgccga catcaccaaa gaagatcgtg gtactgaaat caccctgcat
    19681 ctgcgtgaag gcgaagacga gttcctcgat gactggcgcg tgcgttccat catcagcaaa
    19741 tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa agatggcgaa
    19801 accgttatct cctgggcaaa aatcaacaaa gcgcaggcgc tgtggactcg taacaagtcg
    19861 gaaatcaccg atgaagagta taaagagttc tacaaacaca tcgcccacga ctttaacgat
    19921 ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag cctgctgtac
    19981 atcccgtccc aggctccgtg ggatatgtgg aaccgtgatc ataaacacgg cctgaaactg
    20041 tacgttcaac gcgtgtttat catggacgac gctgaacagt tcatgccgaa ctatctgcgc
    20101 ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg tgaaatcctc
    20161 caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt gctgcaaatg
    20221 ctggaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttctggca acagtttggc
    20281 ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat cgccaaactg
    20341 ctgcgttttg cttctactta taccgattct tctgcgcaga ccgtgtctct ggaagactac
    20401 gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcactgcaga cagctatgcg
    20461 gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga agttctgctg
    20521 ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga cggtaaaccg
    20581 ttccagtcgg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga agttgatgag
    20641 agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgcgtgaa agctctgctc
    20701 ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc agcgatcgtt
    20761 tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc ggcgggccag
    20821 aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact ggtgaaacgt
    20881 gcggcagata ctgaagatga agccaagttc agcgagtggg tagaactgct gctggatcag
    20941 gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg tcgtatgaac
    21001 cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc atttttcttt
    21061 tcatcatctg tactttccgc aaattatctc gccattaacc gtttcagcct caggtgcctt
    21121 tcttgaggca atcgcctgtt ggtggtattg tttatcgctt tttcaaaaaa atcgacacat
    21181 tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc ggggaaaggg
    21241 actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac tggcgatatg
    21301 ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga cattatggat
    21361 gctggcaaac tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg cattgctcag
    21421 gaagactgcc gtaatggttt cctgttggac ggcttcccgc gtaccattcc gcaggcagac
    21481 gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt accggacgaa
    21541 ctgattgttg atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg tgtttatcac
    21601 gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga agaactgact
    21661 acccgtaaag acgatcagga agaaaccgtg cgtaaacgtc tggttgaata ccatcagatg
    21721 actgcaccgc tgatcggcta ctactccaaa gaagcggaag cgggtaacac caaatacgcg
    21781 aaagttgacg gtaccaagcc ggttgctgaa gttcgcgctg atctggaaaa aatcctcggc
    21841 taatcaagcc gcagcagatc tgctttcggg caggtctgct catccacctc aggcaatacc
    21901 tctcacagca attagttctt cttcctcact tttccgctac aattatcaac aagttgaatc
    21961 gataagaggc ggtaatgcgt cagactaaaa ccggtatcct gctggcaaac ctgggtacgc
    22021 ccgatgcccc cacacctgaa gcggtaaaac gctatctgaa acaattttta agcgacagac
    22081 gcgtggttga tacctcacgg ttgttatggt ggccgttgct gcgcggcgtg attttgccgc
    22141 tgcgctcgcc gcgtgtggcg aagctgtacg cctctgtctg gatggaagac ggctcgccgc
    22201 tgatggttta cagccgccag caacagcagg cgttggcaca acgtttaccg gatacgccag
    22261 tggcgctggg catgagctac ggctcgccat cactggaaag cgccgtcgat gaactcctgg
    22321 cagagcatgt agatcatatt gtggtgctgc cgctttatcc gcaattctcc tgttctacgg
    22381 tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg caaacgtagc attccgggga
    22441 tatcgtttat tcgtgattac gccgataacc acgattacat taatgcactg gcgaacagcg
    22501 tacgcgcttc ttttgccaaa catggcgaac cggatctgct gctgctctct tatcatggca
    22561 ttccccagcg ttatgcagat gaaggcgatg attacccgca acgttgccgc acaacgactc
    22621 gcgaactggc ttccgcactg gggatggtac cggaaaaagt gatgatgacc tttcagtcgc
    22681 gctttggccg ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa atgctcggag
    22741 aaaaaggcgt aggtcatata caggtgatgt gcccgggctt tgctgcggat tgtctggaga
    22801 cgctggaaga gattgccgag caaaaccgtg aggtcttcct cggtgcgggc gggaaaaaat
    22861 atgaatatat tccagcgctt aatgccacgc cggaacatat cgaaatgatg gctaatcttg
    22921 ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga agagcctcgt
    22981 cggcggtttt catcatccgt gaataatgca aaaaggcatg cagcgtacct gggtagagtt
    23041 tgaactcaca gggctgctga tgcgccgcta acgtctggta aagcagacgg ctgtcatcca
    23101 gcagcggatc gaactcctcc ccggcaataa aacagggcgg aacttcgcga gtgagatcat
    23161 tattaaacag acagtaatac ggtgactcgc ggtccgcgtc gttgcttaaa tacgcctctt
    23221 catacatctg caaatcctgt tgcgttaagc catcccagac accgcccaac agacgacgag
    23281 tcacggaatc ccgtaatccg taaagcccat accacagcaa aacacccgca attttaccgc
    23341 aatcgatctg tttatcacgc aaccacaacg cactggcgag cgccagcatg gcacctgcgg
    23401 aatcaccggc aaagccaatg cgggacatat tgatttgata atcctccgcc tgctggtgga
    23461 aataacaaca agcagccaca atttcctcta tcgcttgtgg aaaacgcgct tcaggtgaaa
    23521 gggtgtaatc aataccaatc accgtacatt ggctgtagct tgccagcagg cgcatgatgc
    23581 gatcgtgggt atcgagattg ccgagaataa aaccgccttc catgcaaata aaatagcgtc
    23641 gccgggctat ctggctgcgg acaaaagaga cgtgtttcca cctgcccata ttttgttgga
    23701 accatgtaag ctctggttgc catttctgga gcgcccgcat tccagaatcg gcgctcaagc
    23761 gtgtaatact gtcgttgctc agcaatcgtt cccgttgcgg gccaggacgg taaatccggc
    23821 tgaagagtat tcacaacggt cttcatttca gcagaaataa ggtccagaac aggtagtttg
    23881 ttttccggct tcatacaaaa actcctttca aattacgtca ttgtaaggaa ccactgccat
    23941 gaaaatgcga tcccgcctgc tgatattgaa actggctgcg tctcgcgcgc tcccgtcaga
    24001 ttgtgttaac attcgccgct cagttaacca cccgtaaaaa caaccatgaa atttcccggt
    24061 aaacgtaaat ccaaaaatta cttccctgta aatgcacgcg atccgctgct tcagcaattc
    24121 cagccagaaa acgaaaccag cgccgcctgg gtcgtaggta ttgaccagac gctggtcgat
    24181 attgaagcga aagtggatga tgaattcatt gagcgttatg gattaagcgc cgggcattca
    24241 ctggtgattg aggatgacgt agccgaagtg ctttatcagg agctaaaaca gaaaaacctg
    24301 attacccatc agtttgcggg tggcaccatt ggtaacacca tgcacaacta ctcggtgctc
    24361 gcggacgacc gttcggtgct gctgggcgtc atgtgcagca atattgaaat tggcagttat
    24421 gcctatcgtt acctgtgtaa cacttccagc cgtaccgatc ttaactatct acaaggcgtg
    24481 gatggtccga ttggtcgttg ctttacgctg attggcgagt ccggggaacg tacctttgct
    24541 atcagtccag gccacatgaa ccagctgcgg gctgaaagca ttccggaaga tgtgattgcc
    24601 ggagcctcgg cactggttct cacctcatat ctggtgcgtt gcaagccggg tgaacccatg
    24661 ccggaagcaa ccatgaaagc cattgagtac gcgaagaaat ataacgtacc ggtggtgctg
    24721 acgctgggca ccaagtttgt cattgccgag aatccgcagt ggtggcagca attcctcaaa
    24781 gaccacgttt ctatccttgc gatgaacgaa gatgaagccg aagcgttgac cggagaaagc
    24841 gatccgttgt tggcatctga caaggcgctg gactgggtag atctggtgct gtgcaccgcc
    24901 gggccaatcg gcttgtatat ggcgggcttt accgaagacg aagcgaaacg taaaacccag
    24961 catccgctgc tgccgggcgc tatagcggaa ttcaaccagt atgagtttag ccgcgccatg
    25021 cgccacaagg attgccagaa tccgctgcgt gtctattcgc acattgcgcc gtacatgggc
    25081 gggccggaaa aaatcatgaa cactaatgga gcgggggatg gcgcattggc agcgttgctg
    25141 catgacatta ccgccaacag ctaccatcgt agcaacgtac caaactccag caaacataaa
    25201 ttcacctggt taacttattc atcgttagcg caggtgtgta aatatgctaa ccgtgtgagc
    25261 tatcaggtac tgaaccagca ttcacctcgt ttaacgcgcg gcttgccgga gcgtgaagac
    25321 agcctggaag agtcttactg ggatcgttaa gttatcgtcg gttcgtaggc cagataaggc
    25381 gttcacgccg catctggcat ttggctctcg atgcctgatg cgacgctggc gcgtcttatc
    25441 atgcctacat atttttcata ttttacatcc ggcaaccacc gtttaccccg tcaccacttc
    25501 acccgccggt ggcgtttcca gcagttccag catagtacgg gcaatttcac gttcacccat
    25561 cactacctga ttcgcgccac gttcggtgat atacgtcact tcatcgtcat aatgggcgcg
    25621 ggcgataatc tcaatatccg ggtttttcgc gcgggcagac gccacaatct cacccgcttc
    25681 ataaccgttg gggatcgtca ggatcagcca ttttgcacat tccagatgtg ccagttgcat
    25741 aatttcttcg ttcgctgcat tgcccaatac agcgcggacc ccacgctctc gcagctcatc
    25801 aacacgggtt cgtgacgtct caatcaccac cagcggaata tcagaggcga gcaatttctc
    25861 ccccagcagg ctgcctacac gaccgtaacc caccaatagc gcatggttgc aaatatccac
    25921 tgggatctgt ttctcttctt cgattgcctc ttccagcgtc tgctcttcca gcgtttcggt
    25981 cttcgccaga tatttctcca gtagtgcgaa cagtaccggg ttgagcataa tcgacaggat
    26041 cgcccctgcc agtaccaggt tttgtccggc ctgcggcagt aaattcaatg ccattcccag
    26101 tcccgccagg ataaacgcga actcaccaat ctgcgccagg ctggcggcga tggttaatgc
    26161 cgtacgttgg gagtgaccaa acagacggac aaggaacagg gccgcgaccg atttaccaaa
    26221 cagaataatt gccagcgtcg ccagcactgc cagcggttgc tgaatcagaa ttaacggatc
    26281 aaacaacatc ccgactgaga caaaaaacag caccgcaaac gcgtcgcgca atggcaacgt
    26341 atcgtgggcg gcacggtgac tcaattcaga ctcgttcagt accatcccgg caaagaacgc
    26401 accgagtgca aaggagacat caaacagctc taccgcacca aaggcaatcc ctaacgccag
    26461 cgccagcact gacagggtaa acagctcgcg agaaccggtt gccgcgctgc gtgccataat
    26521 ccacggcacc agacggcgac ctaccagcat cataatggcg ataaatgcga tcaccttgcc
    26581 gatggtgatc cccatatcga ctgcaagagt ggcaaagccc acatcgccct gttccatcat
    26641 tcctgccact gcgggcagca acaccagcgt cagaaccatc accaggtctt ccacaatcaa
    26701 ccaaccgatg gcgatttgcc cacgctgact gtctattaat tgccgttctt caagtgcgcg
    26761 cagtaacacc acggtactgg cggtggaaag acataagccg aacacgatac cggtcattaa
    26821 cgaccagccc agcacggcgg agagcgccat acccagcagc gtcgccacgg ctatctgggc
    26881 gatcgcgccg ggaatggcga tggactttac cgccatcaaa tccttgagcg aaaaatgcaa
    26941 accgacgccg aacatcagca aaataacgcc cagttcagcc agttccggcg caagcttggt
    27001 atcggcaaca aagcccggag tgaatggtcc tgccagcaca cccgctaaca gatatcccac
    27061 cagaggagaa atacgtagtt tattggccag catgccgagg ataaaggcga gcacaaggcc
    27121 gccaacaatg gtggtgataa gcggggtggc gtgatgcatt ccgtctcctt ttcctggtgg
    27181 ttattgtcca tttttggccg ggaaaaccaa aattacaggt aatagtttat gacaatttca
    27241 ttgatgatgt ttatgaataa ttgttgaatt ttgcagaaaa atggaattag ctgcaaaaaa
    27301 agcacggatc ggaaaacaga aggcggttta acagaggaaa ggcttatggc gactgtaaaa
    27361 cgatgcagcc aaagtttgcc tttggctgca atgaaatcag tctttatgcc ggttatcagg
    27421 caggaatatg gtcaacatcc ccaatagtgg caggaaagca cagattttat agactaactc
    27481 gatgctggtg tgatcggcga taagccccag aactgccgct cccagacctc ccatgccgaa
    27541 agcaaaaccg aaaaagagtc cagaaatcat accgatacgt cccggaagta gctcctgagc
    27601 gtagaccaga atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac
    27661 ccccgtccag tgcaggcggg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat
    27721 agagccccaa atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt
    27781 acctgccgca acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa
    27841 tccgaatttt tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata
    27901 tttcgagaaa atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag
    27961 tggattgatt atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca
    28021 acggctaatt tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac
    28081 gttgcctttg ccataaggcg cgataatcac cgccgccagc aaggggccca gggaactgcc
    28141 aaagttgccg ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat
    28201 acgggctacg cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc
    28261 cgccagcaga actgcgccaa aactgcccgg cagcgcaagc agcaccagac cacttaaggt
    28321 aaagcacatg ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac
    28381 cactggttgc agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat
    28441 aaatgtcaga gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat
    28501 catgtcgttc agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc
    28561 cttggtcgtt gacgcagccg cgcccgccac aggctgggtt tgttcactca ttgccatagg
    28621 aaagtcactt tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac
    28681 taacatacct gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc
    28741 tggatgcaga tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt
    28801 tttaacacgt tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc
    28861 gttagcgctg ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga
    28921 taaaacctac aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa
    28981 tgaatatggc gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga
    29041 ggttgcggct gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt
    29101 gcccgagtct gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta
    29161 tgacgcgatg gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca
    29221 ggaaaagtgg gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtattggcga
    29281 gcgcctgttt aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg
    29341 gctgacaacc gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt
    29401 tcgtaagccc gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc
    29461 agacattatt atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa
    29521 cgcaccgggc gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt
    29581 cggtggtcac tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta
    29641 cgtgccgggt acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca
    29701 tgagtggggc aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat
    29761 ggttaactac cagctgattc cggtgaacct gaagaagaaa gtgacctggg aagacgggaa
    29821 aagcgagcgc gtgctttaca ctcctgaaat cgctgaaaac cagcaaatga tctcgctgtt
    29881 atcaccgttc cagaacaaag gcaaagcgca gctggaagtg aaaataggca aaaccaatgg
    29941 tcgtctggaa ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat
    30001 tctggcagcc caaatggatc gcactggtgc cgactttgcg gtgatgagcg gaggcggaat
    30061 tcgtgattct atcgaagcag gcgatatcag ctataaaaac gtgctgaaag tgcagccatt
    30121 cggcaatgtg gtggtgtatg ccgacatgac cggtaaagag gtgattgatt acctgaccgc
    30181 cgtcgcgcag atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt
    30241 ggcgaaagac ggcaaactga acgaccttaa aatcaaaggc gaaccggtcg atccggcgaa
    30301 aacttaccgt atggcgacat taaacttcaa tgccaccggc ggtgatggat atccgcgcct
    30361 tgataacaaa ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta
    30421 tatccagaaa agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg
    30481 gcagtaatcc gaaagtgccg gatgtttgca tccggcacaa tgcttaatcg cggcgggcga
    30541 tatcagcaaa tttggcatcg aggatctttg ccagatcgcc tgccgccagt tcgatatcca
    30601 gtccgcgctt gccgccggaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa
    30661 tcgttggcag acgttttttc tgccccagtg ggctaatccc cccaaccagg tatcccgtcg
    30721 aacgctgcgc gaccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta
    30781 cttttttaag atccagttga cctgcgaccg gcgtaacggc cacggcaagg tgtttcatat
    30841 caccgttcac tgccaccagc agcgttttgt agacctgatc cggattcaaa cctaattttt
    30901 tgacgacttc atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa
    30961 acgaaatctt gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt
    31021 aaacaaaaaa acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc
    31081 ctaagagtat tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag
    31141 tgacggcata ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc
    31201 atttttttag cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac
    31261 cgcgaccaca tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg
    31321 ctgatgcatc agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt
    31381 attttgcggc ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt
    31441 atgccagtgg gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa
    31501 catggcaatc tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc
    31561 cgcctgcaat agctggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg
    31621 cgtcgcctga agaaccatcg cgatttgcca cagcggttgg gtagaaaaga gtgagggaga
    31681 aatgcccatc tcctggctaa tgtgctgcag gttttggagt tgctcctcgc taatgcgctc
    31741 ttccagcgcc tcgcaggcag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc
    31801 ctcgacgagc agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc
    31861 catatcgtgg ctacccatat gaatactgcc aatcagatga aaatggcgat tgccggggag
    31921 ggtgatatcg atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt
    31981 tacccggtac aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg
    32041 agggatggcg caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc
    32101 ttgcatcgct attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac
    32161 cgactacacc tggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca
    32221 atgagttagc agagttgctg catgttcatc gtaatcgcgt cagtgcactg atcaataaca
    32281 atcgtaaact caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg
    32341 attttaggct aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca
    32401 cccaggaaga attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc
    32461 gtgcaaaaaa ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt
    32521 tcacgccgca tccggcatcc gagcaccatt gcctgatgcg acgcttacgc gtcttatcag
    32581 gcctacaaac ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt
    32641 catgcgtcga tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc
    32701 cttcggttta aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc
    32761 cattgccgct ccggcaacta ccgggttaag cagtgttcca gtaaacggcc acaaaatacc
    32821 ggcggcgacc ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat
    32881 gttgcgcagc gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg
    32941 catcagggta atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc
    33001 gacatccgcc tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg
    33061 acgtccttca ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc
    33121 aatcacttca tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc
    33181 ggtcaacatc accagacgat atcccgcttt atgcaggcgt tgcagcgccg ccacgctatc
    33241 actacgcaac ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag
    33301 cacaggcgtt gctccttgcg atgcctgagc tgaaatatcg gcttcaatcg ctttagtatc
    33361 gacttgctga tcatttaaca acgcctgatt acccagcaat aacgcatgac cttctgcttc
    33421 accgctcacg cccagcccgc gcaatgtgcg gaaaccgttg acctgcggta gctgcatatc
    33481 gcctgcttta tcaaggatcg ctcgtgccaa tggatggctg gaaccttgct ccagcgccgc
    33541 cgccagacgc agcgcctgcg cttcgtcaac atcagcaaat gttttcactg cgacaacctg
    33601 cggcttccct tcggtcagcg tcccggtttt atcgaacacg acggtatcga gtgtactggc
    33661 gcgttgcagc gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc
    33721 ggaaataatc gacatcggcg tcgccagtcc cagcgcacac gggcaggcga taatcagcac
    33781 cgtagtggca atcaccaggg tatagacaat ctgcggtgcc ggaccaaaga aataccagat
    33841 tgccgcactg acaagcgcaa taaccactac taccggcaca aatacggctg agattttatc
    33901 cgccagctga ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatcct
    33961 tgatagcgtg gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac
    34021 cactgtcccg gcatggacgc tatcgccttc gcctttttgc tgcgggattg gttcgcccgt
    34081 cagcatcgct tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg
    34141 atcgccggtc gtcaggcgca gcaacatacc tggctgcact tctgccagag gcacgctttt
    34201 ttcaccttcg tcagtaacca ggcgcgccgt cggtggggtt aaatcgagta atttctccag
    34261 tgccttagaa gaacgctgac gtgcgcgtgc ttccagcata tggccgagat tgatcagacc
    34321 gataatcatc gcactggctt catagtaaag atgtcgcgct tccatcggga accactgcgg
    34381 ccacaggttg acgctcatcg aatagagcca cgccacgccc gttcccagcg ccaccagcgt
    34441 atccatcgtc gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc
    34501 ggcgaaaacc atcaccgcca gggttatcag gccgataacc aaccacaggc tgcggttgtc
    34561 ggcggtgacc atcatgttat cgccgatcat cccccagacc atcaccggga tacccaccgc
    34621 cagtgcgaca attgcctgcc agcggaagcg cttcatcgta gcgacggcgg tttcttgctg
    34681 gcgttcgcgg cgtttcgcgt catcttcaat cgcttccgcg ccgtagcccg ctttttccac
    34741 cgcctgcact aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag
    34801 gtttacccgt gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac
    34861 acagctggcg cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt
    34921 cgctgccgga agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc
    34981 cagcggttta gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc
    35041 gatcagctgt tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc
    35101 ttgttccaca tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca
    35161 ggacaggccg tccaggttca ggtcgatagt ttgtgacata aaacactcct ttaagacagt
    35221 tttgactggc tgtgataaag gttaaacctt ccagaaaggg gaaggtcaag aaattaataa
    35281 accaggcggg taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga
    35341 catttatttc tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct
    35401 gaaaatcggc acaggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag
    35461 atgttagatg caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca
    35521 cttaacggcg gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg
    35581 gcggcagtgg ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac
    35641 cgctttgcac tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc
    35701 ggcccgcagg cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca
    35761 gttatcgcct tagagttgca tggcggcaaa ccgctttcgc cactggtaaa tgctggcgct
    35821 attgccacca ccagcctgat taacgctgaa aacgccgaac aacgctggca gcgaatttta
    35881 catatccaac agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg
    35941 gaacaaacaa ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat
    36001 ctctattgtg atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc
    36061 aatactgttg aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg
    36121 acgcataaac gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg
    36181 gaagggctgt atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc
    36241 ggtgtaggtg gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca
    36301 ccaccgctgg acgaagaagg caatagtgtt cgcggtcaaa aaatggtggc atcggtcgct
    36361 aagcaactcg gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg
    36421 gtaacaaacc cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg
    36481 cggggatctt cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctggggcg
    36541 cctttgcttt tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg
    36601 cgagctatcc cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg
    36661 tcttttcgct agcgctctcg ttgctatacc tgttgacgct ggcggtgagc atcgccatgg
    36721 ttgcccgtgc ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc
    36781 accttatttt gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa
    36841 gcaaccatgc ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt
    36901 tattgctgat tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc
    36961 cccccagctc cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct
    37021 ttggcatgat ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg
    37081 cgtttctggt ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc
    37141 ttagcgatgt atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg
    37201 cttctccgct gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga
    37261 cggcttcagg cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca
    37321 gcgaacgcga actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca
    37381 acatcatcgt cgtggtgttg ataatgctga tgacggcggc gctaaattta gactcactcg
    37441 ccagcgttgc cagcgccacc tttttgatct gctatctggc ggtgtttgtg gtggcgatcc
    37501 gcctgcgtca tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt
    37561 tgttggtgat cgttggcttt atctacagcc tgtggtccca gggcagccgt gcgttgatat
    37621 ggattattgg tgcaatctta ctcagcctta ttgtggcaat ggtcatgaag cgtaataaaa
    37681 ccgtataaca cctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc
    37741 gcactggcaa aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc
    37801 gttgcgcgaa cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc
    37861 attcgcttct atgaagagaa ggggctgatg acgccgccga tgcgcagcga aaacggttat
    37921 cgcacctaca cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg
    37981 ggctttaacc tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac
    38041 agcgccgacg tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag
    38101 gagctgcaat ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac
    38161 agcgccgact gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga
    38221 ttaagacgag acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacata
    38281 cgtacctgca ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat
    38341 atgaccgcgc ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg
    38401 cccgcgctgg tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca
    38461 tttccaccac agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc
    38521 ccaacccagc ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca
    38581 caacaaataa ccatttccgc ccagcatctc ggctgccagc agcaacccgc cgagactgag
    38641 ccagaaaata tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg
    38701 ctgtctttca ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat
    38761 ggcatcatca ctactttgct gttactggag gaaccgatct gctgtaacgc ttcggtgtat
    38821 ttttgcgcta cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc
    38881 attttggtgg cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac
    38941 gccgactgac gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg
    39001 aggatttccg cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc
    39061 gctttcatct gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt
    39121 tcaatacggg tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg
    39181 cgtgagttga tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg
    39241 gtacggatgt tagtcatggt caggttgatg atcgccagct ccagattgct gacttcatag
    39301 gcagcgcgtg gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg
    39361 ttatctttcg agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc
    39421 ttgcgaccaa tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg
    39481 gtatagcgac caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc
    39541 gcgccgacaa tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa
    39601 acctcctgtt gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct
    39661 gattactgaa agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat
    39721 cagaaaaagg gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga
    39781 tatctggcgg gtgatacgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa
    39841 tttaagttaa ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct
    39901 tcattgatca gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta
    39961 aaaccagaaa tctaccgcca gcaagtctct tactgcgccc agacgccgac gctgtttggc
    40021 gatacggtat acgataatct gatctttccc tggcagatcc gtgaccagca gcctgacccg
    40081 gccatttttc tcgattttct cgaacgcttc gctttgccgg acagcatttt gacgaagaat
    40141 atcgccgagc tatctggtgg tgaaaaacaa cgcatctcat tgattcgtaa cctgcaattt
    40201 atgccgaagg ttttattgct ggatgaaata accagtgcgc tggatgaaag taataaacat
    40261 aacgtcaatg agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg
    40321 acacacgata aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc
    40381 ggagaaatgc aggaagtacg ctatgaactc gcataatatt actaacgaat cattagcact
    40441 ggcattaatg ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga
    40501 gaaagatatt ctctggagcg tcgggcgagc gataattcag ctgattattg tcggctatgt
    40561 gctgaagtat attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat
    40621 ctgctttaat gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat
    40681 ctcatcgttt attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc
    40741 agggtcgatt gaatttatcc cgatgcaggt gatccctgtc gccgggatga ttgccggtaa
    40801 cgccatggta gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca
    40861 gcaacagatt caggagaaac tgagtcttgg cgcgacgccg aagcaggctt cagcgatatt
    40921 gattcgcgac agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg
    40981 cttagtgagt ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa
    41041 ggcgattaaa tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac
    41101 cattattgcc tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt
    41161 gactcaattg aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg
    41221 gttcatgtgc cgaacaacgg aatatcaata caacaatgca tacagctggc ggcgatactt
    41281 cgacgccagt gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt
    41341 ctgaccgtct gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc
    41401 ttcattgcgc ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc
    41461 tggattctcc gccacctgct gttgcaactg ttgaatttcc ggcttatcag ccgcctgctt
    41521 cagcagttcg atttgcgcta ccagcccctg gtagcgggtg tcctgatcct gcaacggaat
    41581 ggttttcagc accgcttccg catcttcaga acggttcagc gcaatcagcg tttctgccag
    41641 cagcaggccg atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag
    41701 ggcatcggtg taattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc
    41761 ttcgcgcggc agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg
    41821 gaagccatct accggttgcc cgttctggaa cagatacacg gtcggaatcg cgcgcagacc
    41881 aaactgggcg gcaatcatct gctccgcgtc gcagtccagc ttcgccagaa taaattgccc
    41941 gttgtactgc gccgcgaggc tttccaggat cggggttaac tgcaaacagt gctggctacg
    42001 ttcagaccaa aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag
    42061 gttagattcg ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga
    42121 tattttcttt gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc
    42181 catcacgcgc ccaggcagca ggcgctcgag caccattact gcccaggtca ccagcgttac
    42241 cggatagcgc atcttcggct tctcgctaat aaaagcatgg cgcactttgt cgaccaccgc
    42301 ttccggtccc aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg
    42361 cgtctggttg acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac
    42421 tttaattccg ctgtggcgta gctccatgcg cagcgcgtct gaccacgcct ccagcgcata
    42481 tttactggcc gcgtaagcgc cacgacccgg cgtggagatt aatcccatca ccgatgatgt
    42541 catcacaata cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg
    42601 gtgtgcgccg aaaaagttgg cggaaaactg ctgttccatc tgcgcacggc tgatggtgga
    42661 aagggggcca tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt
    42721 cagggcgatc acctcgtctg ctgcgcgatt aacactttct ggtgaatcca gatcgatcaa
    42781 cacgccggta aatcccatgt tgttcatacg ctcaacatca ttcggttttc ggcaacctgc
    42841 cagcacatga aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga
    42901 acatccggta attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt
    42961 atgagtcatg atttactaaa ggctacaact gcttcgccat ccagtcggca ataaacggct
    43021 gggcgtcgcg gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct
    43081 cttccataaa aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg
    43141 cgctaaaggc ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca
    43201 atggttcagc gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt
    43261 gctgtggctg aaaaccacgc aaaccatcat tgcgcccagt tcaaccagca cccaacgcgg
    43321 ctgatgctgt ttgagcagag ccggaaggcg cgccagccct tgttgcgagg tgtcgccgct
    43381 gatgctggca ttaactaccg acgttttact ctgccactta tcattcaaca aggcaggcca
    43441 ggccgcgctg gcagacattc gatatccggc gctcaggcta tcacccagaa tcaataacgt
    43501 gtccgctgcg gcggcacgga aggttaacag gaccaggaac aggaagggca aatgccagcg
    43561 gaaaacattg ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc
    43621 tccatcctca ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc
    43681 gagtcgggat cgggtaagtc aaccttgctg gcgatcctcg ccgggcttga tgacggcagc
    43741 agtggcgaag tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca
    43801 aagttgcgcg cgaagcacgt cggctttgtt tttcagtcat ttatgttaat tcctaccctt
    43861 aacgcgctgg aaaacgtcga gcttccggct ctgctgcgcg gtgagagtag cgcggaaagt
    43921 cgtaacgggg cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct ggatcatctt
    43981 ccggcacagc tttccggcgg tgaacagcaa cgagtggcgc tggcacgagc ctttaatggt
    44041 cgacctgatg tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca gacgggcgat
    44101 aaaattgccg acctgctgtt ttccctcaac cgtgaacatg gcaccacgtt gattatggtg
    44161 acccacgacc tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag
    44221 ttgcaggagg aagcatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat
    44281 taattgtctg gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca
    44341 gcgatcgcat ggagaagggc ttaagccagc aaagccgtga gtttatggcg ggcgatcggg
    44401 cgttgcgcag ttcacgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc
    44461 tgaaagtcgg caagcagctg actttcgcca caatgacctt tgcaggcgac acaccgcagc
    44521 tggcgaacgt caaagcggtg gatgatatct acccgatgta tggcgatctg caaactaatc
    44581 cccctggcct gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc
    44641 ttaacctgaa aacggacgac accattgacg tgggcgatgc caccttgcgg attgccggag
    44701 aagtgattca ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga
    44761 tgaatctggc ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc
    44821 gttataaatt cggcggcaac gagaaccagc tcgacggcta tgagaaatgg ttgttacctc
    44881 agcttaaacc cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat
    44941 cgatggaacg ctcgcaacag ttcctgctgc tttcggcgct tctgaccttg ctgctggcag
    45001 tggcagcggt ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtggcga
    45061 tcctcaaaac gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt
    45121 tgatggtgct gacgctttca gccgttaccg gtggggccat aggcctgttg ttcgaaaacg
    45181 tgttgatggt gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc
    45241 cgtggctgtg ggcgcttggc accatgacgg tcatctcgct gctggtgggg ctacgaccat
    45301 accgcttgtt gctggcaacg cagcctttac gcgtattacg taatgatgtg gtcgcgaacg
    45361 tctggccgct gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg
    45421 gattaatggg tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt
    45481 tgctgtgcgg tgtgctgggc tggatgctgc tgaatgtact tcgccgcatg acgctgaaat
    45541 cgctgcctct gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc
    45601 agctttcggc attttcgctc tcctttatgc tgctggcact gctgctggtg ctgcgtggcg
    45661 atctgctcga ccgctggcag cagcagctac cgccagaaag cccgaactac tttttaatta
    45721 acatcgccac agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc
    45781 cggaatcatt ttatccggta gtgcgggcgc ggctgacggc gattaacgat aagccgacag
    45841 aaggtaatga agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc
    45901 ccgatcataa tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg
    45961 aagaggggct ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg
    46021 atacccagga gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc
    46081 ggcctaattt ctattttatt ttccctgaag gggcattaga cgggcaaccg cagagctggc
    46141 ttaccagttt ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc
    46201 cgaccattag cctgttagac attggcgcga ttttaaaaca ggtcggtcag gtgctggagc
    46261 aggtaagtcg ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt
    46321 tgctggcaca ggtgcaggtg ggaatgcgcc agcgtcatca ggagctggtg gtatggcgca
    46381 cactcggtgc ggggaaaaaa ctgctgcgca cgacattgtg gtgtgagttc gccatgcttg
    46441 ggtttgtttc cggcctggtg gccgcaattg gtgcggaaac ggcactggca gtgttgcagg
    46501 cgaaagtgtt tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca
    46561 gcggtgcgct gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta
    46621 aggcgctgtt caggcagttt gtggggtgat gacaggtgtt agattgcctg atgcgctacg
    46681 cttatcaggc ctacgaagag cgtgcaatat attgattttg caagattgtg taggccggat
    46741 aaggcgttta cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca
    46801 actgtcatgt cggttttttt aaaggccgaa ggaggtaaac cttcggcctt agctacgttt
    46861 cctcagttat tcaataaact gacggatcag ttccagcagc gcgattagcg ccttaagaac
    46921 gatgagaact ttatcgagaa aagttttcat ccgttgctcc cttaagggag aaagacaatc
    46981 acagcttacc cttacgttgc ctgtaactat gattttcttc atgtgctaga tgaggactgc
    47041 ctctctccaa attagagcac cagaatcagc accacctgta tcctgaccag gacttaaaag
    47101 tacttttccg cactgcggta atcccttgag gcagtatcac tactagcgtc gggattgtat
    47161 cgccaacata gctgtttgaa tatgtaggca tcctgactta taattcactt gctagatgag
    47221 gactgtttaa tttctgcccg gaacaaccaa gtcttgggcg gaaaaaataa aaaaaccgac
    47281 tgtcatgtcg gttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgaa agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgtcgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gccagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tgcagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggcgg cgcgttcatc gttattcatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtgggttgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgccgctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg gattacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 gggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gc
//
LOCUS       DAJTYG010000020        45514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTYG010000020 DAJTYG010000000
VERSION     DAJTYG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.020"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            52..1353
                     /locus_tag="OO568_001237"
     CDS             52..1353
                     /locus_tag="OO568_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6640668.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTLPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(1409..2416)
                     /locus_tag="OO568_001238"
     CDS             complement(1409..2416)
                     /locus_tag="OO568_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6640669.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(2533..3579)
                     /gene="ansB"
                     /locus_tag="OO568_001239"
     CDS             complement(2533..3579)
                     /gene="ansB"
                     /locus_tag="OO568_001239"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6640670.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(3755..4474)
                     /locus_tag="OO568_001240"
     CDS             complement(3755..4474)
                     /locus_tag="OO568_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6640671.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(4658..4984)
                     /locus_tag="OO568_001241"
     CDS             complement(4658..4984)
                     /locus_tag="OO568_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6640672.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            complement(4984..5703)
                     /gene="trmB"
                     /locus_tag="OO568_001242"
     CDS             complement(4984..5703)
                     /gene="trmB"
                     /locus_tag="OO568_001242"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6640673.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5864..6916
                     /gene="mutY"
                     /locus_tag="OO568_001243"
     CDS             5864..6916
                     /gene="mutY"
                     /locus_tag="OO568_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6640674.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAPLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            6944..7219
                     /locus_tag="OO568_001244"
     CDS             6944..7219
                     /locus_tag="OO568_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6640675.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            7284..8363
                     /gene="mltC"
                     /locus_tag="OO568_001245"
     CDS             7284..8363
                     /gene="mltC"
                     /locus_tag="OO568_001245"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6640676.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            8565..9821
                     /gene="nupG"
                     /locus_tag="OO568_001246"
     CDS             8565..9821
                     /gene="nupG"
                     /locus_tag="OO568_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6640677.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGSELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGTFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(9870..12005)
                     /gene="speC"
                     /locus_tag="OO568_001247"
     CDS             complement(9870..12005)
                     /gene="speC"
                     /locus_tag="OO568_001247"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6640678.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            12403..13110
                     /locus_tag="OO568_001248"
     CDS             12403..13110
                     /locus_tag="OO568_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6640679.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAIPLSAAWTAWFA"
     gene            13216..13291
                     /locus_tag="OO568_001249"
     tRNA            13216..13291
                     /locus_tag="OO568_001249"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13249..13251,aa:Phe,seq:gaa)
     gene            13936..14181
                     /locus_tag="OO568_001250"
     CDS             13936..14181
                     /locus_tag="OO568_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640680.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            14584..15746
                     /locus_tag="OO568_001251"
     CDS             join(14584..14845,14845..15746)
                     /locus_tag="OO568_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HCR6640681.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            complement(15743..15883)
                     /locus_tag="OO568_001252"
     CDS             complement(15743..15883)
                     /locus_tag="OO568_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR6640682.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSDPTHVIWT
                     QA"
     gene            16125..16418
                     /locus_tag="OO568_001253"
     CDS             16125..16418
                     /locus_tag="OO568_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6640683.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            16490..17149
                     /locus_tag="OO568_001254"
     CDS             16490..17149
                     /locus_tag="OO568_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6640684.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            17241..17687
                     /locus_tag="OO568_001255"
     CDS             17241..17687
                     /locus_tag="OO568_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6640685.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(17894..18794)
                     /gene="hlyE"
                     /locus_tag="OO568_001256"
                     /pseudo
     CDS             complement(17894..18794)
                     /gene="hlyE"
                     /locus_tag="OO568_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            19167..19586
                     /locus_tag="OO568_001257"
     CDS             19167..19586
                     /locus_tag="OO568_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6640686.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            19586..20853
                     /gene="umuC"
                     /locus_tag="OO568_001258"
                     /pseudo
     CDS             19586..20853
                     /gene="umuC"
                     /locus_tag="OO568_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(20899..21429)
                     /gene="dsbB"
                     /locus_tag="OO568_001259"
     CDS             complement(20899..21429)
                     /gene="dsbB"
                     /locus_tag="OO568_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6640687.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(21575..23116)
                     /gene="nhaB"
                     /locus_tag="OO568_001260"
     CDS             complement(21575..23116)
                     /gene="nhaB"
                     /locus_tag="OO568_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6640688.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREGVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            23337..24056
                     /gene="fadR"
                     /locus_tag="OO568_001261"
     CDS             23337..24056
                     /gene="fadR"
                     /locus_tag="OO568_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6640689.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(24108..25640)
                     /locus_tag="OO568_001262"
     CDS             complement(24108..25640)
                     /locus_tag="OO568_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6640690.1"
                     /translation="MATIDSINKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARHYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            25970..27268
                     /gene="dadA"
                     /locus_tag="OO568_001263"
     CDS             25970..27268
                     /gene="dadA"
                     /locus_tag="OO568_001263"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6640691.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            27278..28348
                     /gene="dadX"
                     /locus_tag="OO568_001264"
     CDS             27278..28348
                     /gene="dadX"
                     /locus_tag="OO568_001264"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6640692.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(28516..29942)
                     /locus_tag="OO568_001265"
                     /pseudo
     CDS             complement(28516..29942)
                     /locus_tag="OO568_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(30552..32288)
                     /locus_tag="OO568_001266"
     CDS             complement(30552..32288)
                     /locus_tag="OO568_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6640693.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYS
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(32383..33297)
                     /gene="ldcA"
                     /locus_tag="OO568_001267"
     CDS             complement(32383..33297)
                     /gene="ldcA"
                     /locus_tag="OO568_001267"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6640694.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            33397..34008
                     /gene="emtA"
                     /locus_tag="OO568_001268"
     CDS             33397..34008
                     /gene="emtA"
                     /locus_tag="OO568_001268"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6640695.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLRYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(34010..34744)
                     /locus_tag="OO568_001269"
     CDS             complement(34010..34744)
                     /locus_tag="OO568_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6640696.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            34945..35199
                     /locus_tag="OO568_001270"
     CDS             34945..35199
                     /locus_tag="OO568_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6640697.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(35249..36709)
                     /locus_tag="OO568_001271"
     CDS             complement(35249..36709)
                     /locus_tag="OO568_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6640698.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNHIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(36646..37203)
                     /locus_tag="OO568_001272"
     CDS             complement(36646..37203)
                     /locus_tag="OO568_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR6640699.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARVLTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(37229..38083)
                     /locus_tag="OO568_001273"
     CDS             complement(37229..38083)
                     /locus_tag="OO568_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR6640700.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(38083..38892)
                     /locus_tag="OO568_001274"
     CDS             complement(38083..38892)
                     /locus_tag="OO568_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR6640701.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(38902..39660)
                     /locus_tag="OO568_001275"
     CDS             complement(38902..39660)
                     /locus_tag="OO568_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6640702.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APIIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(39657..40637)
                     /locus_tag="OO568_001276"
     CDS             complement(39657..40637)
                     /locus_tag="OO568_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR6640703.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGELMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(40637..41659)
                     /locus_tag="OO568_001277"
     CDS             complement(40637..41659)
                     /locus_tag="OO568_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR6640704.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            42725..43165
                     /locus_tag="OO568_001278"
     CDS             42725..43165
                     /locus_tag="OO568_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640705.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(43244..44941)
                     /gene="treA"
                     /locus_tag="OO568_001279"
     CDS             complement(43244..44941)
                     /gene="treA"
                     /locus_tag="OO568_001279"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6640706.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(45261..>45514)
                     /locus_tag="OO568_001280"
     CDS             complement(45261..>45514)
                     /locus_tag="OO568_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="phosphotransferaese subunit; phosphorylates
                     dihydroxyacetone along with DhaK/DhaL; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR6640707.1"
                     /translation="LPQFNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSAL
                     IARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG"
BASE COUNT        11285 a        11281 c        11467 g        11481 t
ORIGIN      
        1 tattaatcaa agcattcaac aactgaaaca acctgcgcag gaggccagtt aatggatagt
       61 tatatcgcga ttacactgtt tggctgtttt tttgtgctgg tctttattgg tgtgcccatt
      121 tcgttctcaa tcggtattgc cactgtagct tcaatgcttt tgatgtttcc ctgggatatc
      181 gcggcaatca ctgtttcgca acgactggct aatggactgg ataactttgc cctgttggca
      241 atcccctttt tcatctttgc cggaacactg atgaacagcg gcggtattgc gatacgtttg
      301 attaacctcg cccaggtaat ggttggccgc gttcctggtt cgcttggtca tgtgaacgtc
      361 ctggcgaata tgatgtttgg ttccatttct ggttcagccg ttgcggcagc tgctgccgtt
      421 ggtggcacac tgaatcccat tcagaccaaa gaaggttatg atccggcatt ttctaccgct
      481 gtaaacgtct catcttgtat tactggatta ctgataccgc cctcaaacgt gttaattgtg
      541 ttttccctga cagctggcgg cgtttctgta gcatcgctgt ttatggcggg atatttgcca
      601 gggatcttaa tgggtcttgc ggtaatgata gtctgcggca ttattgctaa acgccgtggc
      661 tacccgcttt cagaaagagc aacctttgct caggcctgta aagcgtttct ggatgcctta
      721 ccaagtttgc ttctggtgtt cattgtgatg ggcggcattc tgggcggcat ttttaccgca
      781 actgaagcgt cagcaattgc cgttgtctac acatttattc tctcggtgct gatttaccgg
      841 gaagtaaaat ggcgccattt acccaaactg attctggagt cggttgttac cacttcgatt
      901 gtattactgt tgatcggttt ttccgtgggc atgtcctggg caatgactaa tgctgatatt
      961 ccttatatga tcagcgacgc attgatgggg atttctgata acccactgat catattgctg
     1021 ctgattaata tcgtcttatt aattgtcggc atctttatgg acatgacacc ggctgtacta
     1081 atctttacac cgatcttctt gccgattgca caagagctgg gaatggaccc agtacatttc
     1141 ggtattatga tggtggccaa cctttgtatt gggctgttaa cactgccggt gggtagtgcg
     1201 ctatttgttg gttgttctat ctcgggtgtg aagattcaac atttaatcaa accattgctg
     1261 ccattttatg ctgcattgct agtagcgttg atgatgatca cttacattcc gcaaatatcc
     1321 ttgttcattc cacagctact agggctaatg taaaaaataa tttgccggat aactttttat
     1381 attatccggc aaaggcactt aacatgagtt acttaatcac cccattgcgc cgcagttgtt
     1441 cggtatcttt tttccagcgt tcattagctt ggttgatatc gtcaattttc atcaaaatcg
     1501 cgactttttt actgtttatt tctgaaactt tcatcgttaa cggatattgc cctttttcga
     1561 gcgttaccca gtcatcctgc aatttacggc tgagtttttg tgattgcaga aaagtctgtc
     1621 gccgttgttc gttgatatcg aaatctcgtt tctgctgaat cggcacttca taacactctt
     1681 cactctgttt atgacatgcc gcaatcgccg gttgataaac ctctttgtca tatttaaccg
     1741 cgaacgcctc gctatcgttc tgtttattaa actcatcacg ctgttggtga atttttttaa
     1801 aggcttcttc gcgggtcatt tgcttaccgt ttgcatcttt tccccaatat gcatccgctg
     1861 ctttctgcgc cgccaccatt tgcttatcaa gcgctttcac ttccttttcc agcgcaggaa
     1921 tcgagttctt ctgggtattc aacgcagatt caagctgact aaagcgataa ttgaaatctt
     1981 cgctattcac gatcagatat ttattattcg ttttgatatc gtcgaccacc cgcccacgat
     2041 cgctggctgc cgcctgaaaa gagtaaaagt tcaccgacca gccagacgcg ggcgttcctt
     2101 tactggttaa catcgccgag aattttaccg gtttatcttt cgtccaggtc tgttcgagca
     2161 gttcgtaatc tgccaactga ccgacccagg tatagagatc gtcactggaa gagacatcgc
     2221 cttccgctga ccaggtggcc tgattaccct tagcatcaag attttttaag ctgatggcat
     2281 caagcttgag aatgccgtga tactgctttt tgaattgatc ttttaagata ttttcagttg
     2341 gcggttcgcc atctgcccag gcattaccag tcatcaacat aagcagcgcc gtgccaacaa
     2401 tccattgctt tttcacccga aaaccctcgc gcggaataat ttattccggt gattatcatt
     2461 aggataatga aaattggcaa tggacgtaag tctgaagtga aaaagccccg gcacgatgcc
     2521 gggacgaggt gattagtact gattgaaaat ctgctggatc tgctgcggat ctttggtttg
     2581 cgtcagagcc agctgcagca ggacgcgcgc tttttgcggg ttcagcgtgc cagaggcgac
     2641 gaagccgtat ttcgcatcat ccacttcagc atcctgagtg gtagcacccg tcggtacgcg
     2701 ggaagaacgc actactgcag tgccgttttt cgcggcggtt gccagggtgt cgaacacgga
     2761 tttatacagg ttaccattac ccacaccagc gctaacgatg ccatcatagc ccgcatctac
     2821 cagtgcttta gccggaagat cggatgcgtt agcgtagtta taaacgatgc cgactttcgg
     2881 cagctcattc agcttagaga catcgaatgg cgtatcgctg gtgtgcttac gtgccggggt
     2941 acgttggtag tcaatcttac cgttgtgaat gtatcccaga ggaccgtagt taacagactt
     3001 gaaggtcgct acgtcggtgg tgttggtttt ggtgacatcg cgaccgtcca gtacggtgtc
     3061 gttcatcacc accagcacgc cacgattagc ggaggcttta tcagctgcgg tcactaccgc
     3121 gttatacagg ttgaatggac cgtctgcgct catggacgtg gacgggcgca ttgcgccgac
     3181 catcaccacc ggtttgtcgc atttcaccgt caggtcgagg aagtaagcag tttcttccat
     3241 cgtgtcggta ccgtgggtaa tgacgaagcc gtcggtttta tcgcagtcgg cgttaatttt
     3301 tttcgccagt gtcagccaga cattatcgtt catgtcctgg gagccgatat tcactacctg
     3361 ctcgccttta acgttcgcaa tgtcttttag ttgcggcacc gcattaacca gattttctac
     3421 gccaacttta cccgctgtgt agttagattt ggttgcggag tcaccaccac cggcaatggt
     3481 cccgccggtt gctaaaatgg tgatattggg taatgccaat gctgcaccac taaaacccat
     3541 aaccagtgcg gcaagtgccg tctttttgaa aaactccatt tcattcctcc agttacgtga
     3601 acgctacgca ttatccctta gctttgtatg ggaaatttga cgttaaacaa tttacaacgt
     3661 gaatatattt tggagatcta caaagttaga ggcaggtaac aaaacgaaga attaaacggc
     3721 ataaaaaagt attatgccgt cttaaaatag aggattattt taaattcccg accagggctt
     3781 tgcggctatc ttccagagtc acaacgcggc tacaaacatc tttgccaaac tgctggaaat
     3841 ctttttcctg ctttttccac tcggtttgga ttgaggattg cagaccgccc aagcttccca
     3901 gcacattctg taatgggtta ccgccgcttt tcagcaccgc tttcgcgccc atttcattaa
     3961 tgctgtcctg taaaattccg cccattgcct gattcactag ttgctggcct tcggcgcgaa
     4021 cctgatcaat ggctttatag tggaacgtca ggccatcgct gcgcgtttcg ataatgcgat
     4081 tcatctgctc tttaagctgc gcatcaagtt tggtcagacg gctgcgcatt ttgctgctgt
     4141 cgcccatctc ctgaacgata attttatcca gcgcaatacg ggctttctcg acgcggcttt
     4201 tcgcgccttc atcaatccac ggcagcgtgc tacgtagttc agcctgataa tccttcgcct
     4261 gctcgcgctg ggcggcattc agggaatatt gcttaccgtt atacatcacg ttgccgtctg
     4321 gcgtgatcac cagattgccg ttttcgccct tcacctgcac ggtttgcggg ctgacaatca
     4381 catcgtcacg cggcgtgacg ctgcactggt agtcggcgtg agcggtcatt gccgtcactg
     4441 aaagtgctgc cgccagcagc attttgcgca tcatagtctt ccctcaagaa caaatcaggc
     4501 cagcatatgc tggccccaga ttgataacaa agtacgcgtt ttccatgccg gatgcggcgt
     4561 aaacgcctta tcccgcctac tcgatcgtgc atctctgtag gcctgataag cgtagcgcat
     4621 caggcagttt tgcatttgtc atcgcccgta tgctttctta gtcccaccaa acgtcgaaaa
     4681 gttcgctggt gcgtacctca tccagtttgc actcttccag ccacttacgc acaatcgcct
     4741 gatgttcttc ggtgcatttg ccgatttcct gcatgcagat cagaccttcc caggccagat
     4801 aaccgctgcc gtcaaaggcc agtttgttcg gttcgataac ctcgttaata aagtcatcaa
     4861 cgattttatc aatctgttct tccgatgtac cttccgggaa tcgccatgcc accgaaaatc
     4921 ctaattcctg gaattcgtcg atgtgcattt ttttacgcag acgacggcta cggttctttg
     4981 ccattatttc accctctcga acattaagtc ccatactccg tgaccaagac gatgaccacg
     5041 ttgttcaaat ttcgtcaccg gacgtgatgc cggacgcggt acgtaatcat tgctctctga
     5101 caggttttta taaccgtcaa tagaagacat cacttcaagc atatgttccg cataaggttc
     5161 ccagtcggtc gccatatgga atacgccccc cagctgcagt ttgcttttta ccagttcggc
     5221 aaacggcacc tgaacgatac ggcgtttatt atggcgcgct ttgtgccacg ggtcagggaa
     5281 aaagagctgc accatgcgca atgaattgtc aggaatcatt ttatgcagca cttcaaccgc
     5341 atcgtgacac atcacgcgca ggttgcttaa accttcttca tgcgcagaag ccaggcacgc
     5401 accaacgccc ggtgaatgca cttcaatgcc gaggaagtcc tgctcggggc gatctttagc
     5461 cattgccacc agcgacgccc ccatgccaaa accaatctca agcgtcaccg gcgcttcacg
     5521 gccaaaaagc gcggggaaat ccagcatatc ttcactgaac tcaacgccca tcaccggcca
     5581 gtaattttcc agcgcatgtt cctggccttt ggtcagtcgc ccctggcggc gcacaaaact
     5641 acggatacgg cgcagtgggc ggccgttttc atcaaattcc ggtgaaatga cgtcgttttt
     5701 cataaagatt tagtcgcttg tgaaagtgtt ctgaaaacgg gcattatcca aagttagttg
     5761 ccggatgcaa gcatgataag gccgtggctg cggaaagttc cggtttacac cctgccgtcg
     5821 ctgtgctgca atcttgcccc caacaacagt gaagtcggtg accatgcaag cgtcgcaatt
     5881 ttcagcccag gttctggact ggtacgataa atacgggcgg aaaacgctgc cctggcaaat
     5941 tgacaagacg ccctacaaag tatggctctc agaagtgatg ttgcaacaaa ctcaggttgc
     6001 gaccgttatc ccctattttg aacgctttat ggcgcacttc ccgacggtga ccgatctcgc
     6061 caatgcgccg ctcgacgaag ttctccactt gtggaccggg cttggctatt acgcccgcgc
     6121 gcgcaacctg cataaagcgg cacaacaagt ggcgacctta cacggtggta aattcccgga
     6181 aacctttgaa gaagtcgcgc cgttaccggg cgtcgggcgt tccaccgcag gcgcgattct
     6241 ctcgctttct ctgggcaagc actttccgat tctcgacggt aacgtcaaac gcgtgctggc
     6301 gcgctgctat gctgtaagcg gctggcctgg gaaaaaagag gtcgagaata aactatggag
     6361 tttgagcgag caggtgacgc ccgcggtcgg cgtggaacgg tttaatcagg cgatgatgga
     6421 tttgggcgcg atgatttgca cgcgttcgaa gccgaaatgt tcgctctgtc cgctacaaaa
     6481 cggatgtatt gccgccacca acaatagctg gtcgctttat ccgggcaaaa aaccgaaaca
     6541 gacgctgccg gagcgcaccg gctactttct cctgttacag cacgaagatg aagtattgct
     6601 ggcgcagcgt ccgccgagcg gattgtgggg cggtttatac tgtttcccgc agtttgccga
     6661 cgaagaaagt ttgcggcatt ggctggcaca acggcagatt gctgccgata acctgacgca
     6721 actgaccgcg tttcgccata ccttcagcca tttccactta gatattgtgc ctatgtggct
     6781 tcccgtgtcg tcattcaccg gctgcatgga tgaaggcaat gcgctctggt ataacttagc
     6841 gcaaccgccg tcagttggcc tggcggctcc cgtggagcgt ttgttacagc agttacgcac
     6901 tggcgcgccg gtttagcgcg tgagtcgata aagaggatga tttatgagca gaacgatttt
     6961 ttgtactttc ctgcaacgtg aagcagaagg tcaggatttt cagctgtacc ccggcgagct
     7021 gggaaaacgc atctataacg agatctccaa agaagcctgg gcgcagtggc agcacaaaca
     7081 aaccatgctg attaatgaaa agaaactcaa catgatgaat gccgagcacc gcaagctgct
     7141 ggagcaggag atggtcaact tcctgttcga gggtaaagag gtgcatatcg agggctatac
     7201 gccggaagat aaaaaataaa aacagtgccg gagcacgcct ccggcagctt gcataaaaac
     7261 aaacacaaca cgcacccgga atgatgaaaa aatatctcgc gctggctttg attgcgccgt
     7321 tgctcatctc ctgttcgacg accaaaaaag gcgataccta taacgaagcc tgggtcaaag
     7381 ataccaacgg ttttgatatt ctgatggggc aatttgccca caatattgag aacatctggg
     7441 gcttcaaaga ggtggtgatc gccggtccta aggactacgt gaaatacacc gatcaatatc
     7501 agacccgcag ccacatcaac ttcgatgacg gtacgattac tatcgaaacc atcgccggga
     7561 cagaacctgc cgcgcatttg cgccgggcaa ttatcaaaac gttgctgatg ggtgacgatc
     7621 cgagttcggt cgatctctat tccgacgttg atgatattac aatttcgaaa gaacctttcc
     7681 tttacggtca ggtggtggac aacaccgggc agccgattcg ctgggaaggt cgcgcaagca
     7741 acttcgcgga ttatctgctg aaaaaccgtc tgaaaagccg cagcaacggg ctacgtatca
     7801 tctacagcgt caccattaac atggtgccga accaccttga taaacgtgcg cacaaatatc
     7861 tcggcatggt ccgccaggcg tcacggaaat atggcgttga tgagtcgctg attctggcaa
     7921 ttatgcagac tgaatcttcc tttaacccgt atgcggtcag ccgttccgat gcgctgggat
     7981 taatgcaggt ggtacaaaat actgccggga aagatgtgtt ccgctcgcag gggaaatccg
     8041 gcacgccgag ccgcagtttc ttgtttgatc ctgccagcaa tattgatacc ggcaccgcgt
     8101 atctggcgat gctgaacaat gtttatctcg gcggaattga taacccaaca tcgcggcgtt
     8161 atgccgtcat caccgcctat aacggcggcg caggcagcgt gctgcgagtc ttttcgaatg
     8221 ataagattca ggctgccaat attattaaca ccatgacgcc gggcgatgtt tatcagacgc
     8281 tgacgacccg ccatccctct gcggaatctc gccgttatct ttataaagtg aataccgcgc
     8341 aaaaatccta ccgccgccga taattccatt aaccgcccct gacgatgctc aggggcaaaa
     8401 atgttatcca catcacaatt tcgttttgca aattgggaat gtttgcaatt atttgccaca
     8461 ggtaacaaaa aaccagtccg cgaagttgat agaatcccat catctcgcac ggtcaaatgt
     8521 gctttttcaa acactcatcc gaatcacgat gtgaggaaat taacatgaat cttaagctgc
     8581 agctgaaaat cctctctttt ctgcagttct gtctgtgggg aagttggctg acgaccctcg
     8641 gctcctatat gtttgttacc ctgaagtttg acggtgcttc tattggcgca gtttatagct
     8701 cactgggtat cgccgcggta tttatgcctg cgctactggg gattgtggcc gacaaatggt
     8761 taagtgcgaa atgggtatat gccatttgcc acaccattgg cgctatcacg ctgttcatgg
     8821 cggcacaggt cacgacgccg gaagcgatgt tccttgtgat attgattaac tcgtttgctt
     8881 atatgccaac gcttgggtta atcaacacca tctcttacta tcgcctgcaa aatgccggga
     8941 tggatatcgt tactgacttc ccgccaatcc gtatctgggg caccatcggc tttatcatgg
     9001 caatgtgggt ggtgagcctg tctggcttcg aattaagcca catgcagctg tatattggcg
     9061 cagcactttc cgccattctg gttctgttta ccctgactct gccgcatatt ccggttgcta
     9121 aacagcaagc gaatcagagc tggacaaccc tgctgggcct cgatgcattc gcgctgttta
     9181 aaaacaagcg tatggcaatc ttcttcatct tctcaatgct gctgggctcg gaactgcaga
     9241 ttaccaacat gttcggtaat accttcctgc acagcttcga caaagatccg atgtttgcca
     9301 gcagcttcat cgtgcagcat gcgtcaatca tcatgtcgat ttcgcagatc tctgaaaccc
     9361 tgttcattct gaccatcccg ttcttcttaa gccgctacgg tattaagaac gtaatgatga
     9421 tcagtattgt ggcgtggatc ctgcgttttg cgctgtttgc ttacggcgac ccgactccgt
     9481 tcggtactgt actgctggta ctgtcgatga tcgtttacgg ttgcgcattc gacttcttca
     9541 acatctctgg ttcggtgttt gtcgaaaaag aagttagccc ggcaattcgc gccagtgcac
     9601 aagggacgtt cctgatgatg actaacggct tcggctgtat cctcggcggc atcgtgagcg
     9661 gtaaagttgt tgagatgtac acccaaaacg gcattaccga ctggcagacc gtatggttga
     9721 ttttcgctgg ttattccgtg gttctggcct tcgcgttcat ggcgatgttc aaatataaac
     9781 acgttcgtgt cccgacaggc acacagacgg ttagccacta attacgcaaa gaaaaacggg
     9841 tcgccagaag gtgacccgtt tttttattct tacttcaaca cataaccgta caatcgtttc
     9901 atgccatccg catcggtttc gctatagaca ccttgcaact ccggcgaaaa tcccggcaac
     9961 aaattcacgc cttcttccag cgcaaggaaa taacgttgaa ccgccccacc ccagacttcc
    10021 ccgggtacca cgcaaagcac gccaggtgga taaggcaacg ccccttctgc cgcaattcgc
    10081 ccttcggcat cacgaatccg caccaactcc acttcaccgc gaatataagc gctatgcgca
    10141 tcctgggggt tcatcaccac tgacgggaaa ctctgctggc ggaacatcgc tttttgtagg
    10201 tctttgacgt cgaaactgac atacagatcg tgcatctcct gacacaactg gcgcagggtg
    10261 tagtcgcgat agcgcaccgg atatttgtta taaacgctcg gcaacacctc agccagcgac
    10321 gagtcatcct caatatgctg ttcaaattgc gccagcatcg ccaccagttg tgccagcttc
    10381 tcgtggcttt ccgccggagt taataagaac agaatggagt tgagatcgca cttctccggc
    10441 acaatgccgt tctcacgcag atagtgcgcc agaatcgtcg ccggaacgcc aaagtcgcta
    10501 tattcgccgg tttcggcatt gatacctggt gtagtgagta acagcttgca cggatcaaca
    10561 aaatactgat ccgcggcata tccttcaaag ccgtgccact tcgcccccgg ctcaaaactg
    10621 aaaaaacggc ggtcgctggc taacactgat gtcggataat cctgccacaa tttgccatca
    10681 acaacgggcg ggataaacgg gcggaacagc ttacagcgcg caagaatagc cttgcgcgac
    10741 tcaataccca actcaacgca ctcagcccac agccgacgcc cactctcccc ttcatgaatt
    10801 ttggcgttaa catccagtgc agcaaacagc ggatagaaag ggctggtaga agcatggagc
    10861 ataaaggcgt tattcaaccg cttatgcggg caaaaacgcg cctgtccgcg gatatggtta
    10921 tcttttttat ggatctgcga cgtctgtgag aatccagcct gctgtttgtg caccaactgg
    10981 gtcacaaaaa tccccggatc gttttcgtta agttctaaca gcagcggcga gctatccgcc
    11041 atcatcggga taaactgttc gtaaccgacc cacgcggaat caaacagaat gtaatcacag
    11101 agatgcccaa tggtatcgat cacctgacgg gcgttataga cagtgccgtc ataggttccc
    11161 agctgaataa tcgccaggcg aaacgggcgc ggcaggtcgg ctttttctgg cgccacgtcg
    11221 cgaatttgct ggcgcagata ctcttcatta aaacagtgcg catcaatacc gccaatgaaa
    11281 ccaaacgggt tacgtgaagc ttccagatag accggcgtcg tccccgcctg aatcagcgcg
    11341 ccgtgatgat tcgacttatg gttgttacgg tcgaagagca ccagatcgcc acgcgttaac
    11401 agcgcattcg tcaccacttt attcgctgcc gatgtgccgt tcagcacaaa ataggtttta
    11461 tcggcatgaa agactttggc tgcgaatttc tgcgcatctt tcgccgatcc ttcatgaata
    11521 agcagatcgc ccaattttac gtcagcgtta cacatatcgg cgcgaaagac gttctcacca
    11581 aagaaatcgt aaaaatggcg tccggcagga tgctttttaa agaacgcacc atgttgatgt
    11641 ccagggcaag caaaggtgct gttgcccatc tcaacgtact gcgtcagcgt gtcataaaac
    11701 ggtggcagca aattctcttc atactgacag gctgcggatt ccagctccag ccactgctgc
    11761 tcgttgccgt tgattaccgc cgtaacgccc gcaggtaatt caacagcatg ttcggaatac
    11821 aaaaacaccg gtagatgaaa accggtgcgc ttaagcaacg caagaatgcc actgcgacta
    11881 tccgcagcgg taatgacgac tgccgcgacg tccgtaaaat cagtatctcc caacgccacc
    11941 acgcggcgat gagaagaaag tcgggatacc agttcactac tggcggcaat attcattgat
    12001 ttcataagcg caaacccgtt tcggggaagt aagaataccg gacaaggtgg aaaaccctgc
    12061 cccatgagat atgggtcaaa ctggtcacca gctccgaccg ccagacatca gtaaaagcag
    12121 aaacgctctg attttactgt tgtcctgcag tgagcgtgcg ttaacttcac cgcatgagca
    12181 gtaacataga aagggaaacg tttcgcgcga aacggcgata agcgagagaa tgtaaggaga
    12241 tggcgtgcat cgggcaaact ccgtacagag aggagaaaat tcgcgcaatc atggcacctt
    12301 tcgctaaggc gtgcaagcca ggactttgcg accagacaag ccatcaacaa ggcaaaaagt
    12361 tcataataag aaaatcaaac aatacagcta acaggaactc ttgtggtcat cggccctttt
    12421 atcaacgcta gtgcagtctt actgggtggc gttctcggcg cactgctcag ccaacgctta
    12481 ccggaacgta tccgcgtctc catgacatct atttttggtc tggcatcgct ggggattggt
    12541 attttactgg ttgtgaaatg cgccaacctt ccagcgatgg ttttagctac gctacttggc
    12601 gctctaattg gcgaaatttg tttgctggag aaaggtgtca atacagcagt cgccaaagca
    12661 caaaatctgt ttcgtcactc acgtaagaag ccagcgcatg aatcttttat tcagaattat
    12721 gtcgcgatta ttgtcctgtt ttgcgccagc ggcaccggga tcttcggggc gatgaacgaa
    12781 gggatgaccg gcgatccgag tattttaatc gccaagtcat ttcttgattt ctttacggcg
    12841 atgatcttcg cctgctcgct gggtattgcg gtgtcggtaa ttagtatccc attactgatc
    12901 atccagttaa cgctggcgtg ggctgccgcg ctgatattac cgctgaccac accgtcgatg
    12961 atggcagact tcagcgccgt aggcggttta ttgctgctgg caaccggatt acgcatctgt
    13021 ggcattaaaa tgttcccggt ggtcaacatg cttccagcac tccttctggc aataccgctt
    13081 tccgccgcct ggaccgcctg gtttgcctga caatgcgtgc aatagcggca aagtgatgat
    13141 agattgtgca gtctgcagta aattgaagaa atttgattga cgagacgagg cgaatcaggt
    13201 ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc aggggattga aaatccccgt
    13261 gtccttggtt cgattccgag tccgggcacc actatttaga agaaccagcc tacgggctgg
    13321 ttttttgctt tctggggttc tggtagatat ccggtcactt tgaatgctga tgtcactgct
    13381 ttacctcttc catccagcct gaacatttct tctaagtagc ccccttctgg tcactgtggc
    13441 cttttctgtc aaacttcaaa tccccaatag acactatttc cacatgagca cctggctaac
    13501 ggattcatta ctgcacggat acatccggtc tttgtccggt ctgaagtaaa acgcatacca
    13561 cctgcgttgg cccctcctca gtccccccct acatcaaacc gaacattttt cactctgatt
    13621 gatttagctt tagtcaactt ttgggtgtta tgcctactat atcaatatga tccatagggg
    13681 attatggcca gactaccggt aataccgaga gacaggatct caatcggaga tagaacgttc
    13741 tgcaaaacgg ggcttacaga aaatccctaa atgacctgca acctccagcc cattgcatat
    13801 tatgataaca gtgaatattt tggttaagtc gcgccccgca aaaggtggaa agcaatttaa
    13861 caaattgaat taaaagggtt taaccgcaaa atggacgcaa caggcaacac gacagtcacc
    13921 tgcctgagtc acaaaatgaa gtacaaagaa gtcgcctgcg gtaagcagcg gatgcactgg
    13981 ctggcgctgc agttcgtgga tgagaacaac aacccagttt ccggtctcaa cgtgcagctg
    14041 gagtatcatc cacgggcaat ggcggcagag ctggctctgt gggccagagg cagacacact
    14101 cagtttgacc ccacaccgcc gcccccacct gtcggtgtca ctgacagtca gggtctggta
    14161 cggtttgatg acttgtactg aattaaagca cctaaaacaa acatttatat cgcaacagtt
    14221 aaatagaatg gataatcggt aataaaatgg tccttgagtg cgttagatca acatcaaaat
    14281 aattaaaata cccatgcatt tttttaagat atggttttag atcagaatga gattaaaagg
    14341 gagagaaagt catagcctta tttgatgtca gaaaccaatg cgtggaagtt agagcattac
    14401 ggaaaaaagc ctgacatact taacgggcag gcatagaggt gatctcattg aatttgttag
    14461 cgcaatcagt agatcacaaa actctatgcc tgtcttcttt tctggggact cctcagcctg
    14521 atcttaccca gcaatagtgg acacgcggct aagtgagtaa actctcagtc agaggtgact
    14581 cacatgacaa aaacagtatc aaccagtaaa aaaccccgta aacagcattc gcctgaattt
    14641 cgcagtgaag ccctgaagct tgctgaacgc atcggtgtta ctgccgcagc ccgtgaactc
    14701 agcctgtatg aatcacagct ctacaactgg cgcagtaaac agcaaaatca gcagacgtct
    14761 tctgaacgtg aactggagat gtctaccgag attgcacgtc tcaaacgcca gctggcagaa
    14821 cgggatgaag agctggctat cctccaaaag gccgcgacat acttcgcgaa gcgcctgaaa
    14881 tgaagtatgt ctttattgaa aaacatcagg ctgagttcag catcaaagca atgtgccgcg
    14941 tgctccgggt ggcccgcagc ggctggtata cgtggtgtca gcggcggaca aggataagca
    15001 cgcgtcagca gttccgccaa cactgcgaca gcgttgtcct cgcggctttt acccggtcaa
    15061 aacagcgtta cggtgcccca cgcctgacgg atgaactgcg tgctcagggt taccccttta
    15121 acgtaaaaac cgtggcggca agcctgcgcc gtcagggact gagggcaaag gcctcccgga
    15181 agttcagccc ggtcagctac cgcgcacacg gcctgcctgt gtcagaaaat ctgttggagc
    15241 aggattttta cgccagtggc ccgaaccaga agtggccagg agacatcacg tacttacgta
    15301 cagatgaagg ctggctgtat ctggcagtgg tcattgacct gtggtcacgt gccgttattg
    15361 gctggtcaat gtcgccacgc atgacggcgc aactggcctg cgatgccctg cagatggcgc
    15421 tgtggcggcg taagaggccc cggaacgtta tcgttcacac ggaccgtgga ggccagtact
    15481 gttcagcaga ttatcaggcg caactgaaac ggcataatct gcgtggaagt atgagcgcaa
    15541 aaggttgctg ctacgataat gcctgcgtgg aaagcttctt tcattcgctg aaagtggaat
    15601 gtatccatgg agaacacttt atcagccggg aaataatgcg ggcaacggtg tttaattata
    15661 tcgaatgtga ttacaatcgg tggcggcggc acagttggtg tggcggcctc agtccggaac
    15721 aatttgaaaa ccagaacctc gcttaggcct gtgtccatat tacgtgggta ggatcactat
    15781 ggcctttgct gaactcaaca ttgacattct tacccgcagc aaagctcgtg gaggttagca
    15841 gtgcaagcag caatacagcc ttactgatgc tcttgatttt cattggtagt ctcctgttca
    15901 gaatgagtac gatgaactgt tataatataa caatccctta cgggagaatt ccagtggctt
    15961 ttctgcgttc ggtaaaatcc cggcgcggca ttttttgccg gggggattac ttataaaggg
    16021 gcatggactc aacaaaccgc agaaatctac agctggctgg cttgtggcgc gggtttcatt
    16081 gatgttagct tatgccaaaa cctggcatct taaagagtaa aagtatgttt tgtgtgattt
    16141 atcgaagcag caagcgtgac cagacctatt tatatgtcga aaaaaaagac gatttttcgc
    16201 gtgttcctga ggaactgatg aaaggttttg gtcagcctca gttagcgatg attctgccgt
    16261 tggatgggcg gaagaaactg gtcaatgctg atattgaaaa ggttaaactg gcattaaccg
    16321 agcaaggtta ctatttgcag ttaccgccac cacccgaaga tttgctgaag caacatcttt
    16381 ccgtcatggg gcagaaaaca gacgacacta acaaataacc gatatccggc ggtggcatta
    16441 tctttgtcgg cgcgggtttt catatccacg ataaggtgag gggaacgtta tgtatcaaca
    16501 tcacaactgg caaggtgcgc tgctggatta tccggtgagt aaagtagtct gtgttggcag
    16561 taactatgcc aaacatatta aagagatggg cagcgcagtg cccgaagagc cagtgctgtt
    16621 tattaaacca gaaacggcac tgtgcgatct gcggcagcca ttggcgatcc catccgattt
    16681 cggttcagtt catcatgaag tcgaactggc ggtgttgatt ggcgcgacgc tgcgtcaggc
    16741 aacggaagag catgtccgca aagccattgc cggttacggc gtggcgcttg atctgacatt
    16801 gcgtgatgtt cagggaaaaa tgaagaaagc cgggcagccg tgggaaaagg ctaaagcgtt
    16861 tgataactct tgtccgcttt ccgggtttat ccccgcggcg gaatttaccg gcgatccaca
    16921 aaatacaacg ctgggcctga gcgtaaacgg cgaaaaacgc cagcaaggta cgactgcgga
    16981 catgatccat aaaatcgttc cgttgatcgc ttatatgagc aagtttttta ccctcaaggc
    17041 cggtgacgtt gtgttgacag gcacgcctga tggcgtcggc ccgttgcaaa gcggtgatga
    17101 gctgacagtc actttcgatg ggcattcttt gacaactcgc gttttgtaat actttttgcc
    17161 gccagaaagc ggcggcaaaa cttgcatcgc tgtgccagac tggttataag gtgcgtttta
    17221 acgtaatggc ggaacacctg atgagcgatg tacctttctg gcaaagtaaa accctggacg
    17281 aaatgagcga tgcggaatgg gagtcgttgt gtgatggttg cggtcagtgt tgcctgcata
    17341 aactgatgga tgaagacacc gacgaaatct acttcactaa cgtcgcctgt cgccagctca
    17401 atattaaaac ctgtcaatgt cggaactacg aacgtcgttt cgagtttgaa cccgactgca
    17461 ttaaattaac ccgtgaaaat ctgccaacat tcgaatggct gccaatgacc tgcgcttatc
    17521 ggttgctggc ggaaggtaaa gatttacctg cgtggcatcc gctacttact ggttcgaaag
    17581 cggcaatgca tggtgaacgt atctctgtgc gtcatatcgc agtgaaagaa tcagaagtga
    17641 ttgactggca ggatcatatc ttaaataaac ctgactgggc gcagtgataa atttaataga
    17701 tgctgatggg ggctttctta atatacgtct ggatgctcgc catttcgtag ccctgttttt
    17761 gaaagcgctc tgtcataatg agagttaccc gataccttat tatcaacagg taactctcaa
    17821 tctttaaaaa acgtctgtgt ttttaaatgt gataaacctt ataccttgag tacatggaga
    17881 gaataatcgc ttatcagact tcaggtactt caaagagtgt ctttttaccg tgtcttttct
    17941 gatactcatt acaggtgtta atcatttttt tggccgcttc ttttagcaaa gaaagcatta
    18001 aatcatcata atcaacatag aatctggttg tttcagtttc cgtttttatc tccccgatgg
    18061 cggctatttc ggtggttaat ttcaatttgg cggcatcgat atctttattc gcttgtttaa
    18121 ccgtgttaga cagggtggta aagaaactct gcacagattt taacttgttc ttcaattctg
    18181 gaatcagttt cccttcaact acgcccgcag caatagaata ggaaatgatt aaaccaaatg
    18241 gaccggcgac gacatcggct gcggcaccgg catacgcttc cttcctgatt ttatctacct
    18301 gtgactggaa atagctgctt ttttctgaaa aatcattggt taactggcta tctaacgcca
    18361 gcagtttccc ggaagcgttg ttgaaacttt gtgagcttac cagcagggat ttttgcgctt
    18421 cattcagctt cgtgatgccg tcatccagta ccttaatgag gggcggatgc tttcttctca
    18481 ttgtactcat caaatagcaa aatatacgct gcgagcaatt gcgtcgcaac accacaccat
    18541 tcatacactg tttgggttgc ttcaaaatac ttatcctggc tatccataag taaggtttta
    18601 atatcgccga ctaaaacgga ggctgcctgt gaatactcct gtttaaagcg acttaactct
    18661 tttatggttt catcaaaggt ctgccagggg atgacctgat cgagatattt attataaaga
    18721 tctaatgctc catctgcggt ttcgattgcg tttttaacta cttctaccgt tttatctgca
    18781 acgatttcag tcataatcat tcgcctcttt aaatatataa attgtaatga aactcctgtt
    18841 ttacaactat taataaattt tacttcatct aattcatagt tagccgggcg ggatgcgtca
    18901 atgtctttat ttctattaat atgataaata tcaaacaatg tttaatgtca ttatggcgaa
    18961 tgcttctatt ctattttttt gccgggtgat atttttcatt tctgctggat gagcgtcgtc
    19021 gccagaaggc cacgtgagca caggataaga gaacgaaaaa tcagcagtct atgcagcgac
    19081 aaatattgat agcctgaatc agtattgatc tgctggcaag aacagactac tgtatataaa
    19141 aacagtatag cttcaggcag attattatgt tgtttatcaa gcctgcggat ctccgcgaaa
    19201 ttgtgacttt tccgctattt agcgatcttg ttcagtgtgg ctttccttca ccggcagcag
    19261 attacgttga acagcgcatc gatctgaatc aactgttgat ccagcatccc agcgcgactt
    19321 acttcgtcaa agcaagtggt gattctatga ttgatggtgg aattagtgac ggtgatttac
    19381 tgattgtcga tagcgctatt accgccagcc atggtgatat tgtcatcgct gctgttgacg
    19441 gcgagtttac ggtgaaaaaa ttgcaactac gcccgacggt acagcttatt cccatgaaca
    19501 gtgcgtactc gcccattacc atcagtagcg aagatacgct ggatgtcttt ggtgtggtga
    19561 tccacgtcgt taaggcgatg cgctgatgtt tgccctctgt gatgtaaacg cgttttatgc
    19621 cagctgtgag acggtgtttc gccctgattt atggggtaaa ccggtggttg tgctatcgaa
    19681 taatgacggt tgcgttatcg cccgaaacgc tgaggcaaag gcgctgggcg ttaaaatggg
    19741 cgatccctgg ttcaaacaaa aagatctgtt tcgtcgctgt ggtgtggttt gctttagcag
    19801 caattatgag ctttacgcag acatgagcaa tcgggtgatg tcgacgctgg aagaactatc
    19861 gccccgcgtc gagatttaca gtattgatga agcattctgc gatctgacag gtgtgcgtaa
    19921 ttgtcgcgat ctgactgatt ttggcagaga aattcgcgca acggtgctac aacgtaccca
    19981 tcttactgtt ggtgtgggga tcgcccagac caaaacgttg gctaagcttg ccaatcatgc
    20041 ggcaaaaaaa tggcagcggc agacgggtgg ggtggtggat ttatcaaatc tggaacgcca
    20101 gcgtaaatta atgtctgctc tccccgtgga tgacgtctgg gggattggac ggcggatcag
    20161 caaaaaactg gacgcgatgg ggatcaaaac cgttctcgat ttggcggata cagatatccg
    20221 gtttatccgt aaacatttta atgtcatgct cgaaagaacg gtgcgtgaac tgcgcggcga
    20281 accctgtttc aactggaaga gtttgcaccg acgaagcagg aaattatctg ttcccgctcg
    20341 tttggtgaac gcatcacgga ttatacgtcg atgcggcagg ccatttgtag ttacgctgcc
    20401 cgggcggcgg aaaaacttcg cagtgagcat caatattgtc ggtttatctc cacatttatt
    20461 aagacgtcac catttgcgct caatgaacct tattacggca atagcacgtc ggtaaaactg
    20521 ctgacgccca ctcaggacag cagggatatc attaacgctg ctacgcgatc tctggatgcc
    20581 atctggcaag cgggccatcg ctaccaaaaa gcgggcgtga tgctggggga tttcttcagt
    20641 cagggagtcg cgcagctcaa tttattcgat gacaatgcac cgcgccccgg gagtgagcaa
    20701 ttgatggcgg taatggatac tctgaatgct aaagagggca gaggaacact ctattttgcc
    20761 gggcagggga tccaacagca atggcagatg aagcgagcta tgctttcacc acgttataca
    20821 acgcgaagtt ctgatttact gcgggtcaaa taaatatagc ggcaggaaaa aagcgttccc
    20881 gcaggagcgc cgaatggatt agcgaccgaa cagatcacgt tttttcgctt taaacggctg
    20941 ggaaatcacc accagcaccg cgacaatcag gtaagcgata aaaataccga gcagccactg
    21001 cggcatttcc atacctaaaa aatcccactg acgctcggcg caatcgccag aggcgacaaa
    21061 cacttgcggc acccatttat ccagcggcag ccattccggg aaacgaacca taaaatcaca
    21121 ggtggcaaat ggcgaaggat agagctgaag catggtgtgc tcgtaagtta actgcacacc
    21181 gcggaacgca ctatacaacc agataaccat cgctacataa cgcagcggag ttttcggggc
    21241 gatcgcgcca atcagcgcag cacccagaac gccgaataac gcgcagcgtt cataaataca
    21301 gagcacgcaa ggtttcagta acatcacatg ctggaaccac agcgccgtca gttccagtgc
    21361 cagagcagta aacgccatca acagccacgc gccccggcct tgtgaacatt ggttcaaaaa
    21421 tcgcaacata atcatttccc tgcaatatgc atagagtgcg cagtttaaac caattcattc
    21481 gctgcgccac caggaggcag caaaaatagg acgcaattgg acaattatcg ggcgaaaagg
    21541 caaaccgggc atttatatgc ccggtaagtt gttatcaaag cgttgctatc cagcccattt
    21601 gcataaacca ttcggttaca ggggcaagcg taaactcgac gcagagcaag ccgacgagtg
    21661 tcagtacgag ggtgtaaggc agggccatcc acaccatgcg gccataagag aggcgaatca
    21721 atggcgcgag tgcagaggtc agcaggaaca ggaacgcagc ctgaccgttc ggcgtagcga
    21781 cagagggcag attggtaccg gtattaatgg cgaccgccag cagctcgtat tgcttcaacg
    21841 tgatagcgcc actttccatt gccgctttcg cttcgttgat ataaatcgtc cccacgaaga
    21901 cgttatccga aatggatgac agcagaccgt tgaaaatata gaacagcgac agctgagcat
    21961 gttcagatgc ctgcaacaca aactgaataa tcggcgaaaa cagttgttgg tcgataatca
    22021 ccgcgactac cgagaaaaac accgtcaaca gtgcggtgaa tggcagagat tcggtgaagg
    22081 ctttaccgat agcatgttca tcggtgacac cggtcaatga tgttgccaga ataatgactg
    22141 acaaaccaat caagccaact tccgccagat gcaacgccag cgcagtcacc agccagacgc
    22201 caataatcgc ctggacaatc agacggattt tatcctgacg ggtacgctgg tggcggcttt
    22261 gatcgtcaaa ctgttgcaac acttcgcgga ctttctccgg cagcgtttca ccgtagccaa
    22321 accaacgcag cttctctacc agcaggcagg ttaacaggcc acaaatcaga accggaacgg
    22381 tcaccggcga catgcgcagg aagaaatcgc caaaatgcca gccagccgct ttagcgatga
    22441 tcaggttctg tggttcgccc accatggtca ttacgccgcc taatgcggtg ccgacaccgg
    22501 catgcatcat caggctacgc agaaagccac ggaactgttc cagaaccacg ttgtaatgct
    22561 tgtcgatatg actatcgtct tgcaggtcgg tgtcttcggt acgggaagag gctacgcgat
    22621 gataaatacc ataaaaaccg actgcaacgc tgatcaccac cgccacgacg gttaaggcat
    22681 cgaggaacgc ggagaggaac gctgccgcca cgcaaaaaga gagcgacagc agcattttgg
    22741 agcgaatgct taacagcaaa cgggtaaata tgaacagcaa cagctgtttc ataaaataga
    22801 tacccgccac cataaacatc aataacagca agacttcaag atttgccgcc accccttcac
    22861 ggacgtgttc cgcgctggtc atgccgatga ataccgcttc gatagccaac agaccaccgg
    22921 ggagtagcgg gtagcatttc agggccatcg ccagagtgaa aataaattcc gctaccagca
    22981 accagcccgc gacgaaaggg ctgatgagga aaattaacgg gtttacgatt aagaaaatga
    23041 tgagggcgag tttgtaccag tcgggggact ggcccaaaaa attgcgccat agcgcgcggc
    23101 cccaggagat ctccatgatg gtttccctta ccttacaaat aatcaatgat gtttttgtgt
    23161 ttaaacgcaa agcttaacgg tcaggcagga gtgaggcaag tcttgatagt caaggggaaa
    23221 gagatgcgga aaatgaagcc ttgatccctt tttcttcttt ttgtctgcta tcagcgtagt
    23281 tagccctctg gtatgatgag tccaactttg ttttgctgtg ttatggaaat ctcactatgg
    23341 tcattaaggc gcaaagcccg gcgggtttcg cggaagagta cattattgaa agtatctgga
    23401 ataaccgctt ccctcccggg actattttgc ccgcagaacg tgaactttca gaattaattg
    23461 gcgtaacgcg tactacgtta cgtgaagtgt tacagcgtct ggcacgagat ggctggttga
    23521 ccattcaaca tggcaagccg acgaaggtga ataatttctg ggaaacttcc ggtttaaata
    23581 tccttgaaac actggcgcga ctggatcacg aaagtgtgcc gcagcttatt gataatttgc
    23641 tgtcggtgcg taccaatatt tccactattt ttattcgcac cgcgtttcgt cagcatcccg
    23701 ataaagcgca ggaagtgctg gctaccgcta atgaagtggc cgatcacgcc gatgcctttg
    23761 ccgagctgga ttacaacata ttccgcggcc tggcgtttgc ttccggcaac ccgatttacg
    23821 gtctgatcct taacgggatg aaagggctgt atacgcgcat tggtcgtcac tatttcgcca
    23881 atccggaagc gcgcagtctg gcgctgggct tctaccacaa actgtcggcg ttgtgcagtg
    23941 aaggcgcgca cgatcaggtg tacgaaacag tgcgtcgcta tgggcatgag agtggcgaga
    24001 tttggcaccg gatgcagaaa aatctgccgg gtgatttagc cattcagggg cgataatccc
    24061 ttccgtttaa agagcaaacc cctcaaacga ggggtttttt gttgttttta cagatttccc
    24121 attcttggcg ggcaacgttc cagcaactcg atgctgccgt cttcgttttg ctgttcgagc
    24181 atcacatcaa atccccacag gcgatgcaca tgcttcagga cttctttgcg cccccgatcc
    24241 agcggtgcgc gattatgtgg aatataacgc agcgtcagcg aacggtcgcc gcgcaaatcc
    24301 acgttccaga tctgaatatt cggctccaga ttacttaagt tatattgcga cgataaccgg
    24361 ttacggatct cccgataacc ttcttcatta tgaatagcgg aaatctccag ataattatgc
    24421 cgatcatcat ccagcacggt gaagaagcgg aaatcacgca tcactttcgg tgacagaaac
    24481 tggctgataa aactctcatc tttgaaatca cgcatcgcga aatgcagcgt ttccagccag
    24541 tcggaaccgg cgatatccgg gaaccagtat ttgtcttctt ccgttggcga ctgacaaatc
    24601 cgtttaatat cctggaacat ggcgaacccg agggcatacg ggttgatgcc gctgtaccac
    24661 gggctgttat aggggggctg gaagaccaca ttggtgtggc tgtgcaaaaa ctccagcata
    24721 aaacgttccg ttactttccc ttcatcatac agatggttaa ggatggtata gtgccagaag
    24781 gtcgcacagc cttcgttcat cacctgagtc tgtttttgcg gataaaaata ctggctcacc
    24841 ttacgcacaa tacgcaggat ttcacgctgc catgattcca gcagtggcgc atttttctcc
    24901 ataaaataga gcaggttttc ttgtggttcg gacggatagt ggcgcgcttc agcaaccgtt
    24961 ttctcttcct cgcgcttcgg cagggtacgc cagagcatat tgacctgact ttgtagatac
    25021 tcttcgcgac ttttctgccg ggctttctct tcttgcagcg agattttttg cgggcgtttg
    25081 taccggtcca cgccgtagtt catcagcgcg tggcacgagt ccagaagccg ttctacttca
    25141 tcaacgccat aacgttcttc gcactcggta atatatttac gggcaaaaat cagataatcg
    25201 acaatcgaac tggcgtcggt ccaactgcgg aataagcaat tgtttttaaa gaaagagtta
    25261 tgcccatagc aggcgtgtgc catcaccagc gcctgcatag taatggtgtt ctcttccatc
    25321 aagtaagcga tacacgggtt agagttaatg acgatttcat aggccagccc ttgctgaccg
    25381 tgcttataca gccgttcagt ctcgataaac tttttaccga atgaccagtg cggatagtta
    25441 attggcatgc cgacgctgga ataggcatcc atcatctgtt ctgaggttat cacttcaatc
    25501 tggtgcgggt aggtatccag ccggtagagt ttcgccaccc ggtctatctc tgccagataa
    25561 acatccagca ggtcgaacgt ccagtcgggt ccatcgctca aacgtgtggt gtccttatta
    25621 atagaatcga tcgtcgccat acgcgcacct cattgttgtc ggcgctctct gtgtggagca
    25681 cctcatttca agcatagaac acctgttaaa aaccgcgtcg ccggagaatt tttttctttg
    25741 cgatttctta ttatcagagt gccactaatc cgcttctgaa cggaatttta tgctggataa
    25801 aaagggcgtt cagcaggaga tactaaagac gccatattgc cgcagagtca gggagatgtg
    25861 agtcagctca ccataaaaaa gccgtatgtt gaataatatt ttcaactgag ttatcaagat
    25921 gtaattagat tattattctt ttactgtatc taccgttatc ggagtggcta tgcgagttgt
    25981 catactggga agtggtgtgg taggcgttgc cagcgcctgg tacttaaatc aggcaggaca
    26041 tgaggtcacc gtcattgatc gggagccggg ggccgccctg gaaaccagtg ccgcgaatgc
    26101 cgggcaaatc tcccccggat atgctgcacc gtgggcagca ccaggtgtgc ctttaaaagc
    26161 gattaaatgg atgttccagc gccatgcgcc gctggcggtt cgtctcgacg gtacgcagtt
    26221 tcagttgaaa tggatgtggc aaatgttacg taactgcgac accagccact acatggaaaa
    26281 caaagggcgg atggtgcgtc tggcggaata cagccgtgat tgcctgaaag cattgcgcgc
    26341 cgaaaccaat attcagtatg aagggcgtca gggtgggacg ctgcaactgt tccgtaccga
    26401 acaacagtat gaaaatgcga cccgcgatat cgccgtgctg gaagatgccg gcgtaccgta
    26461 tcagctgctg gaatccagcc gcctggcgga agtggatccc gcgctggcag aagtagcgca
    26521 caaactgacg ggcggcctgc agttacccaa tgatgaaacc ggagactgcc agctgtttac
    26581 ccagaatctg gcgcggatgg cagagcaggc gggggttaaa ttccgcttta atacgcccgt
    26641 tgaccaactg ctttgcgacg gcgagcaaat ctacggcgtg aagtttggcg atgaagtgat
    26701 taaggccgat gcgtatgtga tggcgtttgg ttcttactcg acggcgatgc tcaaaggcat
    26761 tgttgatatt ccggtgtatc cgctgaaagg ctactcgctg actattccaa ttgcgcagga
    26821 agatggtgcg ccagtatcca ccattcttga tgaaacctac aaaatcgcca ttacccgttt
    26881 cgataaccgc attcgtgttg gcggaatggc ggaaattgtt ggttttaaca ccgagctgtt
    26941 gcaaccgcgt cgtgaaacgc tggaaatggt ggttcgcgat ctctatccac gcggcggtca
    27001 tgtcgagcag gcgactttct ggacgggttt gcgcccgatg acgccagacg gtacgccggt
    27061 tgtcgggcgt acacgcttta aaaatctgtg gctgaatacc ggtcacggca cgctcggctg
    27121 gacgatggct tgcggttccg gtcagttgtt aagcgatctg ctctctggtc gcacgccagc
    27181 gatcccatat gaggatctaa gcgtagcgcg ctacagccgt ggatttacgc catcacgtcc
    27241 gggccattta catggcgcac acagctaagg aaacgagatg acccgtccga tacaggccag
    27301 cctcgatctg caggcattaa aacagaatct gtccattgtc cgccaggccg cgccgcacgc
    27361 gcgcgtctgg tcggtggtaa aagcgaacgc ttacgggcat ggtattgagc gtatctggag
    27421 cgcgctcggg gccaccgatg gctttgcatt acttaacctg gaagaggcaa taacgttacg
    27481 tgagcgcggc tggaaggggc cgatcctgat gctggaagga tttttccatg ctcaggatct
    27541 ggagatttat gaccagcacc gcctgaccac ctgcgtacac agcaactggc agctcaaagc
    27601 actgcaaaat gcgcggctaa aagcaccgtt ggatatttat cttaaagtga acagtgggat
    27661 gaatcggttg ggcttccagc ccgatcgcgt gcttaccgtc tggcagcagt tgcgggcaat
    27721 ggcgaatgtt ggcgaaatga ccctgatgtc gcattttgct gaagcggaac atcctgatgg
    27781 aatttccggc gcgatggcgc gtattgagca ggcggcggag gggctggagt gtcggcgttc
    27841 gttgtccaat tcggcggcga ctctgtggca cccggaagcg cattttgact gggttcggcc
    27901 tggcattatt ttgtatggcg cttcgccgtc cggtcagtgg cgtgatatcg ccaataccgg
    27961 attacgtccg gtgatgacgc taagcagtga gattattggt gtccagacgc taaaagcggg
    28021 cgagcgtgtg ggctacggcg gtcgctatac tgcgcgcgat gaacagcgaa tcggcattgt
    28081 cgccgcagga tacgccgacg gttatccgcg ccatgcgcct accggtactc ctgttttagt
    28141 ggacggcgtg cgcaccatga cggtggggac cgtctcgatg gatatgctgg cggttgattt
    28201 aacgccttgc ccgcaggcgg ggattggtac gccggttgag ctgtggggca aggagatcaa
    28261 aattgatgat gtcgccgccg ctgccggaac ggtgggctat gagttgatgt gcgcgctggc
    28321 gttacgcgtc ccggttgtga cagtgtaact tattgtaagc cggatcggag gcagcgtctt
    28381 ctggttgcga aaaaatcatc catccggctg gtcagcaact gtagttgtta atgtgacaga
    28441 gccattgccc atgatagtgt ccattaaaag aatggacact atttccccgg aacctgaact
    28501 cgccgcacag gcgttctaca taaaacgctt acgcttcatt attgactcgg agtcgactta
    28561 gataaatcac gcttcaccct tgatgcagat tcgggttcat gttcagattg ttgattaaca
    28621 ggctgcgagt cctgttgtct ttccagactc aacaggccca actgttgatc caggagctcc
    28681 aggtcctcct gtaccttcac gcgctccagg agttccgggt catccaggaa ctccagatcc
    28741 ataaggtcct ccagctcctc gagtccttcc aggtcaacct agccttcctt gtgcaaaaag
    28801 tccaaaaggt ccttcaggcc ctgctgtttt ttcaggccct cgacggcttt gagcagcgtc
    28861 ggtaaattgt ctttgtccca cgggttacca ttacagtcaa acaccttcag gctctggggc
    28921 tgcggcacct gtaccgtttt aaacttgttt tctccacagt agtaaattat tagtttgggc
    28981 ggcagccgtg gtgaagagat caaatcgtta ctagaacagt caaatacttt caggcgagtc
    29041 aagttctcca gcatctcatc aggtattgag ttaatattgt tgccggcaca ggatagttta
    29101 tacaggtact ggggcatcga cggcggtaaa aattgcaaga agttttcaga acagttaatc
    29161 actctcaggt tagtcgacag ctcaggcagc cagataatac agttttcata gcacttcagt
    29221 accatcagtt ttgagggctg caccttcggt aacaagatca agacgttatc tgtacagtca
    29281 agcaccagca gccactttgg catcaccttc ggtaacgcgg tcaacacgtt tttactgcag
    29341 taaaattcaa tcagatgtgg aggcaaattc tcagggagtg aagtcaatcc caggttgcta
    29401 agattgagca tactccgtct tgtaatgaga cattccttca ttctgcgcaa tgcttctgcc
    29461 cggttttcaa tccggcttgc tggcaggttt tcttccagga tattttcctt gatttcttcc
    29521 agactttctt tcaggtcttc ttggggaatt tctgactgtt tgttttgctg gttttcttcc
    29581 aggatttcgt acaggttttt ttgcaggttt tcttgcaaac catcctgctg tatagcccat
    29641 tcgcaccatt tggcgaccat catgaactcg ttatgttgct gattcaggcg agtccccaac
    29701 gtgatgatgg tattgttgtc cacctgtatg ttcactttgc ccgcgatact gaggtcaatg
    29761 atctgccagg gggcatcacc ccagctgaat ttttgcgggc tgagatactt catgttctgc
    29821 aaaatttgat tcttgagttc caccgacagg aagagccgcc ctggcgggcg tcgtacgtct
    29881 gggttaacgg aagatggggg aatggcgggg gcgaatgggc agatgtcttt aaagctagcc
    29941 ataatgctac gtttccttga aataaatatc ctctggcagc gccggagacg tgaatgtggt
    30001 caccgcaaag gagccacagg acgtcatttg cgctgatatc actactcatc gttgactttt
    30061 tccctggtca gaaatgccgt gtcgggtctg gtacatgctg tattacggcc taggtagctc
    30121 tgacgtatta aagaaaatac gagagtgaac gacccggaaa taatcgttat taagcaaagg
    30181 aataactata agaaatcgct catgcatccg gtgctacagg cggatgaaac gcctattgtg
    30241 tgggctatgt cagtgcggca ggttgcgtgg ggaatatgtt caggcgatgt caggcagggg
    30301 cccctgatgg ttgaccggta atgcggggct ggtttttatt acctgttaca gtcggaaaaa
    30361 gcccttacgc tgactttctt gagccggtgt tttgagttca ttagccagag cctgcaatgg
    30421 tttgtcatcc ataatttaac cttcttctga tgttgggatg agcatatcaa aatcaggaaa
    30481 tgacaagttt attacaggct caggctgcgt tcagtaaagc atcgcatccg gcgatagatg
    30541 ctgcattaaa tttaagattc agcttcctct tcagccaccc gaacgccaat cttcaacact
    30601 tcattatctt ctttctcggc caccgtccag atcatcccgg caaactctac ctggtcaccg
    30661 acaaccggtg ccgcgcctag caactgctgg acaatttcac ccagcgtttg ctgtttatca
    30721 cgatattctc gcccgtcttc caggccatat atcagcgcaa catcagaata tttggcgctg
    30781 gcttcgagaa tgaagtcacc aaagaagcgt tgatccagcg cgaccggtgg cgactggctg
    30841 aacagtttac cgagcgccgg gagatcgcgt tcccgaccaa ttacacacaa tacatcgcct
    30901 tcgcgcagtc gggtgctgcc ggtaggatga agcaactggt tatcacgaaa cagggctgca
    30961 atacgcgtct cttttggcat atgcaaatcg cgcagtgccg cacctacaca ccatttatca
    31021 gcactcaatt gataaacaaa ctgctcccac ggattttccg gatgaatatc caggccaacg
    31081 cgtgacaccg gacgtccgac gggcggaacc accactttgg cttttttcgc cgcccacgag
    31141 agtgatgttc cctgcaagag cagtgaaacc agaaccacaa agaaggcgac attaaagaac
    31201 agtcgcgcat tctccagccc cgccatcatc gggaacacgg ccaggatgat tggcaccgcg
    31261 ccgcgtaatc ccacccagct gataaacacg cgctcacgca gattgaaacc gcggaaaggg
    31321 agcaatccag caaataccga aaggggacgg gcgaagaata tcatccatgc cgacaaaatg
    31381 agcgccggaa tggcaatagg cagcagatcg cttgggttaa ccaatagccc cagcaccagg
    31441 aacatggcga tttgcgccag ccaggcgagg ccgtcgaaat tttgcaggat gccgtagcgg
    31501 ttgcgaatcg gccgattacc cagcagaaaa ccgcacagat aaacagccag aataccgctg
    31561 ccttccagcg ccgtggttaa agcaaaaatt aaaataccgc cgcttaatgc cagcaatgga
    31621 tataatccgg caggcagggc gatgcgatta atcatttgca gcagtaaata accaccgcca
    31681 agaccgatga caatcccgag gccaaattgc tgcagaatat cgaccacgaa catccaactg
    31741 acactgcttt catgctgctg gatcattgca atcagggtaa tcgtcagaaa gaccgccatt
    31801 ggatcattac tgccggattc aatttccagc gtcgagccaa cacgttcgtt aagcccctta
    31861 ccacccagca gagaaaagac cgctgcagca tcggtggagc cgacgatagc gccgattaat
    31921 aagccttcaa tcaaatcaag attaaacagc cacgccgcca tcatgccggt taaaccagag
    31981 gtgataagta cgcccagcgt cgccagcgac agcgccggtc ctaacgccac acgaaaggaa
    32041 ctggcctgag tgcgcatccc gccgtcgagc aaaataatcg ccagcgccag gttacttacc
    32101 atgtaggcga aggggtaatt atcaaacggg ataccgccga cgccatcgac tcccgccagc
    32161 atgccgatcg ccagaaaaat aaccagaata ggaatgccaa gacgggaaga aaatgaacta
    32221 agtaatatac tgctggtgac aaggatggat cctaagatga aaaggctaat tattgttgtg
    32281 gcatccaacg gtcggttact cctgattacg ctgtctctta tataaaccct accatattag
    32341 cggcagagac agcgttttac ttagccctga agcgtgattt ttttacattt taagaacagg
    32401 atgaccgcta atcgtcaact gagtgccttc ccgggtgtta ttcagaatgg catgtgcgcc
    32461 caacggcagc gtaaccgtgc gttgttcatg accaaaatcg aggccggtaa tgagtggaat
    32521 cgacaggcgg gaacgcaaaa acgcgtacac tgactccagg ttgtaacctg cgtcataatc
    32581 attgggcgtg ctgccgctaa agctaccgag aataatcgcc ttctgacgtg gcaaaattcc
    32641 cgcatgatag agctgcaaca gcatacgttc gacgcggaaa gggtgctcgt taatatcttc
    32701 cagcaccaga ataccgttct caatttttgg catccacggt gtaccaatca gtgaaatcag
    32761 catcgcaaga ttgcctcccc acaacgtgcc ttcggtctga catgtcgggc cttccccttg
    32821 ccattcaatg gtgaaggttt cattgcgtaa cgccagccag aaatggtgct cggtaaaggc
    32881 gttcagttca tccgcaccaa agtttgctac cagcatgggg ccgctaaagg tgatgacatt
    32941 gccttgcgcc agaagaccgc actgaatggc ggtaaaatcg ctatgtccgc aaatgagtaa
    33001 cgggtcatgt tgttggcgag ccaccagtgc ctgccagtca atatctgcca gtaaacgact
    33061 ggcaccgtaa ccgccgcgta cagccagcac gatggtgttg ggggtagtca gtctggcaag
    33121 ggaattgaga tcttccagac gctccgtttc cgtacccgca aaacgctcac aacgacgggc
    33181 aataacctcg acgttattga cttgatgccc cgcgtcggta aggcgctgga taccacgcag
    33241 cgcggcgtgc tgtttaatgc tgtaacccga tggggcaatt aagtgaaaca gagacatggc
    33301 aattccttgc tgacaacaga aacgaaatgt atatcatgcc gcttaggtgt gccgttgtca
    33361 cctcaacggc gattccaggc tataaggata gaagaagtga aattgagatg gtttgccttt
    33421 ttgattgtgt tattagcggg ttgttcatca aagcatgact atacgaaccc gccgtggaac
    33481 gcgaaagttc cggtgcaacg tgcgatgcag tggatgccaa taagccagaa agccggtgca
    33541 gcctggggcg tcgatccaca attgatcacg gcgattatcg ctatcgaatc gggtggtaat
    33601 ccgaacgcgg tgagtaaatc gaatgccatt ggtttgatgc agttaaaagc ttcaacctcc
    33661 ggacgtgatg tttatcgccg tatgggctgg agtggtgagc cgacgaccag cgagctgaaa
    33721 aatccagagc gtaatatttc aatgggggcg gcttacctga atattctgga aaccggcccg
    33781 ctggcaggca ttgaagatcc gaaggtactg cgatatgcgc tggtggtgtc atacgctaac
    33841 ggggcaggtg cgctgctacg gactttctcg tcagatcgga aaaaggcgat cagcaaaatc
    33901 aacgatttgg atgctgacga gttcctcgat cacgtagcgc gaaatcatcc tgcgccgcag
    33961 gctccgcgct atatctacaa acttgagcag gcactggacg cgatgtaaat cagtcgcgca
    34021 ctttgtccgc tttttcccgg gcttctcgct cgagagagaa aataatccgc tgtaattgcc
    34081 gctccaccgt cgggctgacg ttaagaaaac ggaagctcag acggggagtg gtgatggttt
    34141 cattcttgcc atcaatcact ttgcgctcgc tggtggagat taactgggcg tcaaagtgaa
    34201 aaacacccca ttgccccatg ttgacttcaa tttgagcgaa gcgcatacct tcttgtaatt
    34261 cggcaggctt tgctgtttcc agtaatgcgc ccatgccgcc taacgacaaa tcatacaagc
    34321 ggaaacgtaa cgtactgtta tccgccagtt tggtctggca aaaataaggc ggatggagtg
    34381 gggcggagat gcggaaatat cggcgtcgtt gtacaaacca taaggtggga ggcggtacgg
    34441 taataaatgc cggaagctgc aagtattcac tctgctgtag ttgttcaaca gtaaactcga
    34501 ctttcgcacc ctgagtttcg gcggtaatgg taatgtgctg tgcctttagc acggcgatgt
    34561 tgtcttcggc ttgactgccg aaatccagca ccagtttatc cggggttatt gccagtattt
    34621 tgctgatcag ctgcccgcca ttccaactga gacgcaaagg aattgcggct ttgtgcaaat
    34681 cgcgtaacac gcccaggacg gctaacggat tttgtttcag gaactgctca tggtaatgac
    34741 tcacgcggaa aaactcctga ctgacaaact gtctttggtt tatcggtcat agttaacaaa
    34801 acttaataca aatgcgtgaa tattttttac atgttgttct taaatcagcc gacaaacgcc
    34861 cagcatcgat cccgcccttg cctatactta gagcgttgac gtaagcattt cttgcgaccg
    34921 attcatcgaa acgagggcct gaacatggga attattgcct ggattatttt tggcctgata
    34981 gccggcatta tcgccaagct aatcatgccg gggcgtgatg gtggtggatt tttcctgacc
    35041 tgtattctcg ggatagtcgg tgcggtggtc ggcggctggc tggcgaccat gtttggcatt
    35101 ggcggctcca tcagcggttt taatttgcac agcttcctgg tggcggtggt gggcgctatc
    35161 ctggtgctgg gcgtattccg cctcctgcga agagaataag attttcataa ggcggatagc
    35221 gatacagatg ccgctatccg ctttcacatc agaacgtata ctcgacacct gcccagaaat
    35281 tgcgaccttc ttcaataaac ccttcactgt aggcatattg ggtatcaaac aggttattga
    35341 ccgacgcatt gacgctgaag ccatgaccta aggtgtaatc ggctcgaatg tgggtcaccg
    35401 caaaaccggc ggctttttgt gaaccgtcac tgttgctgta gctggaggaa cgcgcctctt
    35461 ccgacagcgt tacgcttaac ggctcccatg gtttgagtgt catccatgcg gtcattgtct
    35521 gcgttggaag atcggttatc ttgccgatgt ctttgcgttt ggcgtcagcg tggatcagag
    35581 cgtagctcaa tcctacatcc agtatattgc tgattttccc cttaataccg gcatccagac
    35641 cgctgtaatc caccgtgccg ctgttctgat tttgaatggt atcggcattg atattgtgcg
    35701 agaggatggc atcactcacc cggttatagt aaacgctgac ctcaaatccc cagtcgcgcg
    35761 tgaaggcacc attccaggtt aaatccaccc cgcgagcgcg ttccggcttg agctgtgggt
    35821 taacaatcgc tatatggtta tacgcaggtt tggatgtggt atagcgttct ttcagcgtcg
    35881 gaaagcgtgt gcggtcatag tacgaaagcg ccagagtgtc ttcattggca aagtggtatt
    35941 tccccatcac ctgccagtta aaagctgact gattgttgtc gtcataatgg gtgatgctgc
    36001 catctttctc gtgttttttc gcttctacgc tatcgcgcca gtcatagctg attcccgcca
    36061 cgacatcgac attatcagca gcagcccatt gatattcact ggcgagcgac caggtacgat
    36121 cttcatagcg atcgtaagcg gcgtgcggcg cacctttttc ccggtgtacg tcatctttcc
    36181 agttaacggc aaacgacagc agatcgtttt cacgcacatc ggctgccagt tgtaatccgg
    36241 caccgtcgct gtaatcggaa taatggctgt agctgccttt tttatttttc aaatcagcca
    36301 gcgagttgta catcatcagc gtattttcaa aggtgtcgcg atacagccga cttttcaggg
    36361 taaaacggtc gtttagttgg gtcgctccct gataataaaa actttctttg tcatactctg
    36421 gccactgcca gtagcgtgat ttttgaccac aatttccgct gtatggcggg ttatcttttt
    36481 caccatcctg cttaatgtaa gtcagcgtgt attcatcgtt ttcgcgcggt gtaaaaccga
    36541 gcttcacaat gccgcgttta tcatcagccg atgaattaat catcttgccg tgtttgcctg
    36601 caatatcatt atttacacca tgcggcaggc cgagaaaatc ctgctttagc tggctaccgc
    36661 tgacttgcaa atacccaaga tcgctgctgg cggcaaatga agcatgcata tcgtaggcat
    36721 tgtcctgact acggctccat ccctggcgat atcccagact tgcttcttca gggtggtgat
    36781 attaatggct ccgcccatct gattaggccc atgaagcagc gacgaatacc ctttggaaac
    36841 ttcgactgcc cccagattgt tggtcagaac ccgcgccaga tcgaggttgc cgtcataggg
    36901 aacataaatg ggcacaccgt cgaaatagac cggcacctgc cgactatcaa agccacgaac
    36961 ttttacctgt tcttcgttgc ggctgcctga cttttgcagc accacgccgg ggacgacact
    37021 taatgcctga gcgacattct gtttatccaa ggctttcatg gtgggttgat cgagaacggt
    37081 ggtcgtcgtc gatgataccg gactggacca tacggtcagc gtgtcgctct cctgagccgc
    37141 tacggcctgc tgggtaaagg caagtgataa tgctgtgcag agataacgtt ttttgagtct
    37201 catagaataa attccgttta acaaagaatt aaatagaggc tgtgggttgc agactgactt
    37261 taatgtcagc tggtgccgcg taataaggcg cggaggtaat aaaaaggcga atgccgcagt
    37321 cgaggtaatt tttgagtgtc gtcagattaa ttccgccggt cagagccagc gtgcagtgag
    37381 gagccagcga cggcgctatt tgcgcaattt cagttgcctg ttgcggacta aatttgtcga
    37441 gctgaagtac gtctggttgt gcgcgtaacg cggcgatagc ctctttcggc gtgtcggctt
    37501 caacaacaat tttcttctcg ggtgcgtggc gacgtaactg agcgattgcg cctgaccagc
    37561 cctgattgtc atgaagaaaa tggcggtggt tggcaaacaa taatatggtt tccgcacatc
    37621 cggcacgatg aattagtcct ccggcagcca gaattgcctg cgaagccagt aagcgggtgc
    37681 ccggaattgc ttttcgggtg caggcgatat tgccgtcagg ataacgttca cgaagcaatg
    37741 ccagcatttg agcgagataa tcagaaacac cgcaactcca ctccagcaca ttctggactg
    37801 ccttccatcc ctgatgaagt gctgcggcat taccctgcgc acggattagc cgttgacccg
    37861 cgttcgcttg tgaaccgtcg ctgaccgcgt catcaatagt taatcccagc gtagttaaca
    37921 tcttacacgc gacagaaacg ccgctgacgc agccgccctg acggtgaaaa aacgctatat
    37981 agccaggctg gtgtccaata tttaatgctc gcgtggtcag gtcacctccc tggatatctt
    38041 ccagcagtaa cgcatcgatt tgtgcctgag aaaggaaaat catcacagtg cctcctgcgg
    38101 tacgctatcc caccagatga gcgcccagtc gcggctggca ggggcgaggg cacgagcatc
    38161 ttgttgttga taccagtgat ataaccgctc acgctcatcg tcgttaagcg cgccaagact
    38221 ccagctgacg ttctgctcaa aacgttccca ggtgctgtta tcctgttggc aattttgtcc
    38281 acggataaaa tcaacgtggg cataaattcc catctgatac aggacgttga gcgcaaagat
    38341 atagttgggt aattcgatca cttcccgacc agccgcccgc tgaatggctg gagagacgaa
    38401 cgaggtgctg accagatgag ttgtatagac gcgaagccgt gcctgattat tcagtttgct
    38461 cattgcatgg cgcatatcag ccaccagagt ggagcgggaa gccacggcaa tatcgcagcg
    38521 tggcaggtca ctccagtcct cttcccaggc gcgctggatc cagtggacat tgtcagcctt
    38581 cagtgcggcg gcgcggcggg cggcaacgtt cagcatcccc tgactgtaat caacgccata
    38641 aaccgtagtg agtttgtcag ccagcgccag cgatacggtg cctggtccgc agcccatatc
    38701 gaataaggtc tgagcgcctt gcaggtcaat tttagcgatc agttgttgca ggtagctgtc
    38761 cgttggcgag gcgcaatttt ccgccatttt ctccgcccgt tgatcccagt gatcgggcgt
    38821 tttttctgtg cggtgagcca gtttgagttg ctgcagataa aggtctgcaa aatcaatatc
    38881 atcaatgagc atgattgttc cttagcggct aattgcagag aggtgatgat gaatttggtc
    38941 ggcagaaacc cgatacaggg ccgccagttt gttcgttgtt aactgttcgg tcggtaagcc
    39001 ttgtgttact cgtccatctg gctctacctg gataatgctg tcggcaatgg cgttggcatg
    39061 gagcggatgg tgagtcgaca ttaacattgt catgccgtta tttttgagct gcgccagcgt
    39121 gtccagaagt tggatctgat gaccgaaatc gaggctggag gcgggctcat ccagtaacaa
    39181 caagcgcggt tgttggacga gtgcacgggc gattaaagcc agctgacgtt caccgccgct
    39241 aagcgtattc cagcgtcgtg tcgccagatg caaaatgccc agcttttcca gttgctccgt
    39301 ggctttcagg cgttcttgct taccgggaac ggaaaaagcg cctatgatgg gggccaggcc
    39361 catcaacacc atatccagca cactgaaagc aaaaataccg tcgtgcgcct gcggcaccca
    39421 ggcaatagcc tgtgcgcgct ggcgatggct gagtgtctgc acagggaggc tatccagtag
    39481 gatctcgcct tttaataacg gtataacgcc aagaatggag cgcatgagtg tggttttgcc
    39541 gcaaccattc gcgccaagca gacaacaaat tgttccttgc gacagggtga aggacacgtc
    39601 gcgtagcact ggatggcggg agtaacccag agacgcatga cgaacggcaa gcaaagtcac
    39661 tggcgacctc cgcgtaacaa caaaagcagg aaaaacggtg cgccaacaaa agcggtcaga
    39721 atgccaagtg ggatttctgt tgttccaatg ctgcgcgcca gcgtatcggt aagcaggagc
    39781 agaatagcgc cagtacacat tgccataggc aacagctgct gatggttatg accggtcaac
    39841 aagcgggcaa tgtgcggaac gaccaggccg atccaaccaa ttatgcccgc tattgctacc
    39901 gtgctggcgg tgattagtgt ggcgcagaca atcaatccaa agcgcagacg cgtcacgttc
    39961 agtcccagcg aacgggcttc gtcgtcggaa agcgtgagta aattcatgcg ccagcggaga
    40021 aaaaacaggg gcaaggagcc tatgagaatg atcggtgcag catagcagag atcgcgtaag
    40081 gtcacggtgg acagtccccc cagcaaccag aaggtaattg acggtaactg ggtgtaagga
    40141 tcggccagcg ttttaattag cgatatcccc gctccgcaca acgttcctaa cgcaatgcct
    40201 accagcacca gggttaagat cggatcgtgg cgggtcaccc gacgcgtaat caaccagacg
    40261 cccgcgacca ccatcagctc gccgcaaaat gcgtacagct ggatgtaaac aatgggtaat
    40321 ccccacagga ttgccgcgca ggcaccaagt ccggcaccgg cggagacgcc taaaatatcc
    40381 ggtgaaacta agggattacg caacattccc tgataggtgg ttccggctcc tgccagcgct
    40441 gcgcccagta acagcgcagc aagaatacgc ggcacgcgga tttgccagaa gacaatttgt
    40501 tcctgcacag acacattttc ttgacgaaga attagcgcca gcagttgctg catatcgaga
    40561 tgataagccc cacttacggc ggcaaaagtg atactgatag ctgccagcag caaaatacat
    40621 ccgtttacga ttctcattag ctcgccacca atttttgata gtcggcatca ctgagtgaac
    40681 aatgccagaa cagttgggcg taatgctgca tgtcactttt aaactggcga ttgatggcgg
    40741 gatccagcca ggcgtgaagt ctgcgtaatc ccagaagacg gttgattccc ggcggggcat
    40801 cgagccagcc aaagggcaag ccacttaaaa ataagatgcg ttgttccgca acggctttca
    40861 cgccctgcca gagaggatca cgacgaataa aatctgcagt aacggcctcc tgaaccagaa
    40921 taatatccgg ctgccagcgc aggagatttt ccatggaaac ctgtgtcagg ccgtggcgat
    40981 cggctatttg cgcgacgttg tgcaaaccta ataattccgc cgcctcggta tgcaacgaac
    41041 cctgtaagcc cgtttccagc cctcggggac cgcgcgcagc ataaaagcgg aggttagcgg
    41101 cgggtgaggt ggcgaatgcc tgagcttcac caacgaagtg gctggcgaga ttggcttgtt
    41161 ctgcggcgcg gtgctcttcg cctaacgttt tgccaagcgt tgttaactgt tctgctgatt
    41221 gcgccagttt tccgttaagc aataaccagg ggatttgtgt ctgttcgcta acctgccgtg
    41281 cctgggagat caaggtttca tccgtattgc cgcaatcaac gaccaaatcg ggatgtaacg
    41341 ccataagccc ttccagcgat agtgtgctgg cgcgcccggc gagtcgtccc agcctgggta
    41401 actggcgaat gtgctctggc aatggaatta atgcctgacg ggcaaaatca aaagaggaaa
    41461 aaccgaccat tttttctggc gcgacagcca gcaatagcaa atcggcgggc gcgcctgcgc
    41521 tgacgatgcg tgtaatgttt tgggcttcgg gaagagattg cgaagcaaaa cgattaactg
    41581 tttgtgaaaa ggacggtaga gactgcagga gcagggtaga tgcaagtgcc tgcgcaaacg
    41641 tccgtctggt tatgggcatg gtgaacatcc tgaaaagcaa aatcgatata tactttcata
    41701 tatatcgata aaaatcatgt tcaatatcaa agtttagcta ttgaaaacat gaagatgtga
    41761 cgacttccgc aagcctgttg accctctcta tcattaaaaa tgtatttatc aggtggctaa
    41821 tattatttaa atcatttaag gctaaatcat ttaaatatga atgacattat tgttttttat
    41881 cgtccacggt tacgcgacgt tgaagttact tgttttacaa tgagcgattt ttgatatctc
    41941 gccgagaaaa ttatcagcat tctctcgttt cagtgtcgtg aatatactgt taacggaggt
    42001 gaaaagaatg ctgggggtta tacaaaacct atgttgttgt cgtccggatt caccgcttgc
    42061 tgatagtaag cagcgtaacc agaagttacc gcaacaagac tttgaggatt ttaagaacga
    42121 cgttattaat gtcattaaca acaatgaaga tatttatttt cttagagaga gtaatttact
    42181 gaatgcgttt attcgaacgt gcaaaaaaag agatgataac agttacggaa atatgatatt
    42241 tacgctttat tttgataacg atatctatgg gaacagaatt atcaaagcat cgctgaacgg
    42301 cggagagttg cagttattaa gcacagggtg tagcaatgat aacattctcc attttattaa
    42361 tgcatttctc attcgggtga aatttccgct acctggacgc ataaaaccgg aactctcgcc
    42421 aaaagccggg cactccgaag gaattgacgc gttgcgctgc tctgtaacgg catttaccgc
    42481 aagccattta tttcgccagc gacaatccgc agagaaccaa acgcatcagg aaacgactga
    42541 acacaataaa tactgtattg atcaacatat tatactggca cctccagcac gcagtgagga
    42601 tacactgtat catcagcgaa tcttatcact gcggagctgg ttgaacgact ttctgagcga
    42661 aataagtcgg ttcccggtaa caacaacaga atcctttttt aattattgtt tcgttgtttt
    42721 tggtgtgatt cagaaaataa atattttaga cagtagccag tcaggcgtta aaaatggggc
    42781 ggatagtgag gcggttttgg ctgcgcttat tgaacattta gaattgatca atccttctgg
    42841 cagattaagc caaaataccc gttcggcaat gcttcagtta cgcgaagagg aatggtctga
    42901 attctttttt tggttgctaa attctctgga gtgtctggat tatgtgatta ttaatttaac
    42961 tccagaaagc aaaaaaacgt tgatgagtga acaccgtaac aatattcagg tagcaattga
    43021 tgctttatat agccaacgtc ggcgtaaatc gccgggtgat gaaagtgaaa cacttacccg
    43081 tagaaatgac gctatcttcg gcaaccacgt atggcaaaca tttgcgcaat acttcccgcc
    43141 aggacttgaa aaaccgtctg tgtagaaaaa accatagata tggtgttatc tgccgacaac
    43201 gccatccgga gaagccgaat gacgttgtcg gagtaagcgc tggttaaggt gtgggttgtg
    43261 cctctttggt tgagggttgc gtcgttgctg acttaacggt cggacgcgtc gccggaacat
    43321 tgtcacacgg ttgctctttc gggcagatca aatccagcat tttcagcgtc acgccattgg
    43381 tccagccaaa gccatcctgt aatggatatt cgccaccgcc gccccccgtt ccggtggtgc
    43441 tgacatcata tttttccacc agctttttct cccggtcata ggtgtgctga acattggtca
    43501 ggaagtgcca gctaatgtcc atcgccacct ctttttgccc gtagttttgt aatccttctg
    43561 ttgcgaccca ctgtaacggt gcccagccat ttggcgcatc ccattgttgc ccacttttca
    43621 ccgacgtggt gttcaggccg ccgggttgca gcagatgtgt tttcgtcgcc gtcgccattt
    43681 tgttggcgcg atctttcgct gccgcattga cgtacagcgg gaacagggcg gccgcggtta
    43741 actgattgcg cactttatga cttttcaggt cgtaatcggc ataccagcct tgttgatcgt
    43801 tccacaggta tttttcgatc cctttttgac gggcattcgc cagcgtttcg tactggtttg
    43861 ccatcgcgtt atctcctgca gctttgctgg cgcgggcgag gattttttcc attttaaaca
    43921 tcaggctgtt cagatcgacc ggtacgatgc tggtggtgcg taaggtattt aactgctgcg
    43981 ggttgtccat ccagcgcgag ctgaaatccc agccagacgc agcggcagag cgcaggtcgc
    44041 ggtaaatttc agtggcaggt cgattcggat tgcttttggc ggtggcaata tcttccaccc
    44101 atgactctgg tcgtggcgta tcgcgatcgt cccagtagcg gttgagaagg gtaccatcct
    44161 gaagttttac aacgcgtttt tcctgttgtc cggcttgcag gttttcaaca ccgtccatcc
    44221 agtaagcata ttctttttgc atttgcggca ggtattgctt caacgcggca tcgccttcat
    44281 gctgcgccag taactctacc atcagggcaa agaagggcgg ttgcgagcgg cttaaatagt
    44341 aactgcggtt gccgttggga atatgaccgt aagtgtctat ttcatgagca aaattggcca
    44401 ccatatccgc gactttatcc cagtgaccgc tttcggcaag tcctaacatg gtgaagtaac
    44461 tgtcccagta atatacctcg cgaaaacgtc cgcccggcac gacataaggt tccggcagcg
    44521 gtaacagaga atcccatttt tcggtgtttt cggtagaacg cgttaatacc ggccaaagtc
    44581 cgtcaatatg ttcgcgcagt gactgcccct ctggcggaac atatttctcg ccttctttcg
    44641 gcagggtgaa attgacgtta acgaaatggc gcagatcaaa tccgctctgg ttttgctgca
    44701 tccgataatc agcaaggatc atcagcggat cgctgttcgg cacggcatcg gcaaaggttt
    44761 tttggtccgg aaaaagtttg gcgttttgca catcattaaa cagcggccct aataaaatat
    44821 caggcggctg tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca gcgaaacaca
    44881 aaaagataca ggctggaatt aacgccattt tttgcgggcg agaaggtgcg ggggatttca
    44941 tcaatcattc tcctttggcg aaaccgaata aaacggtatt caagtatcag aaaaccttag
    45001 ttcaggatcg cactagctgc atgatcgaaa tctcattctg cgaacggtca ggcattttag
    45061 aaaagggaat taaagggata aataacggcg aaatagagtg atcaaataac gtctgtttat
    45121 tagccagcca gcgacatttg gttcgcgatt agcgaagaaa aatcatcata aattggtaaa
    45181 aaatataaca ggtagttaaa aaacatcagt gctgggtaaa ttgccggatg acatcagaac
    45241 gatgccatcc gaacggtggc ttaaccctga cggttgaaac gttgcgtttt aacgtccagc
    45301 gttagcgttt cttctggttg tatcgcatac agtttctcac cctgctggca aatccagcca
    45361 atccccagtt cacgggcgat tagagcgctg tgggataccg gacttccggc gctaaggcag
    45421 atacctttta caaccgccgg atccagttgc agtactgtgg aaggataaat gttctccgcc
    45481 agtagaatag ttggcgagtt aaactgcggg agtt
//
LOCUS       DAJTYG010000021        44090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTYG010000021 DAJTYG010000000
VERSION     DAJTYG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.021"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(1296..2978)
                     /gene="glcA"
                     /locus_tag="OO568_001281"
     CDS             complement(1296..2978)
                     /gene="glcA"
                     /locus_tag="OO568_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HCR6640708.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFEPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(3333..5504)
                     /gene="glcB"
                     /locus_tag="OO568_001282"
     CDS             complement(3333..5504)
                     /gene="glcB"
                     /locus_tag="OO568_001282"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR6640709.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLCELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMVPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5526..5930)
                     /locus_tag="OO568_001283"
     CDS             complement(5526..5930)
                     /locus_tag="OO568_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HCR6640710.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(5956..7158)
                     /gene="glcF"
                     /locus_tag="OO568_001284"
     CDS             complement(5956..7158)
                     /gene="glcF"
                     /locus_tag="OO568_001284"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HCR6640711.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVENVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIV"
     gene            complement(7169..8221)
                     /gene="glcE"
                     /locus_tag="OO568_001285"
     CDS             complement(7169..8221)
                     /gene="glcE"
                     /locus_tag="OO568_001285"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR6640712.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8221..9720)
                     /gene="glcD"
                     /locus_tag="OO568_001286"
     CDS             complement(8221..9720)
                     /gene="glcD"
                     /locus_tag="OO568_001286"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HCR6640713.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKESLDINPVGRRARVQPGVCNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNYILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            9966..10736
                     /gene="glcC"
                     /locus_tag="OO568_001287"
     CDS             9966..10736
                     /gene="glcC"
                     /locus_tag="OO568_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR6640714.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10860..12002)
                     /locus_tag="OO568_001288"
                     /pseudo
     CDS             complement(10860..12002)
                     /locus_tag="OO568_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12406..14094
                     /locus_tag="OO568_001289"
                     /pseudo
     CDS             12406..14094
                     /locus_tag="OO568_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            14091..15005
                     /locus_tag="OO568_001290"
     CDS             14091..15005
                     /locus_tag="OO568_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR6640715.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            15038..15286
                     /locus_tag="OO568_001291"
     CDS             15038..15286
                     /locus_tag="OO568_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6640716.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLNVKPPAQLMETLLPWLENK"
     gene            15286..16458
                     /locus_tag="OO568_001292"
     CDS             15286..16458
                     /locus_tag="OO568_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6640717.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTDTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFVVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISPGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(16494..17573)
                     /gene="lptG"
                     /locus_tag="OO568_001293"
     CDS             complement(16494..17573)
                     /gene="lptG"
                     /locus_tag="OO568_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6640718.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17570..18640)
                     /gene="lptF"
                     /locus_tag="OO568_001294"
     CDS             complement(17570..18640)
                     /gene="lptF"
                     /locus_tag="OO568_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6640719.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18671..19231)
                     /locus_tag="OO568_001295"
     CDS             complement(18671..19231)
                     /locus_tag="OO568_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6640720.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19243..20067)
                     /locus_tag="OO568_001296"
     CDS             complement(19243..20067)
                     /locus_tag="OO568_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640721.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20067..21416)
                     /locus_tag="OO568_001297"
     CDS             complement(20067..21416)
                     /locus_tag="OO568_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6640722.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21462..22220)
                     /locus_tag="OO568_001298"
     CDS             complement(21462..22220)
                     /locus_tag="OO568_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640723.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22252..22965)
                     /locus_tag="OO568_001299"
     CDS             complement(22252..22965)
                     /locus_tag="OO568_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6640724.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23139..23831
                     /locus_tag="OO568_001300"
     CDS             23139..23831
                     /locus_tag="OO568_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6640725.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23880..25379)
                     /gene="pitB"
                     /locus_tag="OO568_001301"
     CDS             complement(23880..25379)
                     /gene="pitB"
                     /locus_tag="OO568_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6640726.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGVNDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25671..27530)
                     /gene="gss"
                     /locus_tag="OO568_001302"
     CDS             complement(25671..27530)
                     /gene="gss"
                     /locus_tag="OO568_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6640727.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVGHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27735..28601
                     /gene="yghU"
                     /locus_tag="OO568_001303"
     CDS             27735..28601
                     /gene="yghU"
                     /locus_tag="OO568_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6640728.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGNEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28724..28972)
                     /gene="hybG"
                     /locus_tag="OO568_001304"
     CDS             complement(28724..28972)
                     /gene="hybG"
                     /locus_tag="OO568_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6640729.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29106..29447)
                     /gene="hypA"
                     /locus_tag="OO568_001305"
     CDS             complement(29106..29447)
                     /gene="hypA"
                     /locus_tag="OO568_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6640730.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29440..29928)
                     /gene="hybE"
                     /locus_tag="OO568_001306"
     CDS             complement(29440..29928)
                     /gene="hybE"
                     /locus_tag="OO568_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6640731.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29921..30415)
                     /locus_tag="OO568_001307"
     CDS             complement(29921..30415)
                     /locus_tag="OO568_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6640732.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALELVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30415..32118)
                     /gene="hybC"
                     /locus_tag="OO568_001308"
     CDS             complement(30415..32118)
                     /gene="hybC"
                     /locus_tag="OO568_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6640733.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVSGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32115..33293)
                     /gene="hybB"
                     /locus_tag="OO568_001309"
     CDS             complement(32115..33293)
                     /gene="hybB"
                     /locus_tag="OO568_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6640734.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33283..34269)
                     /gene="hybA"
                     /locus_tag="OO568_001310"
     CDS             complement(33283..34269)
                     /gene="hybA"
                     /locus_tag="OO568_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6640735.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34272..35390)
                     /gene="hybO"
                     /locus_tag="OO568_001311"
     CDS             complement(34272..35390)
                     /gene="hybO"
                     /locus_tag="OO568_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6640736.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35579..35866)
                     /locus_tag="OO568_001312"
     CDS             complement(35579..35866)
                     /locus_tag="OO568_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6640737.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35985..36872)
                     /locus_tag="OO568_001313"
     CDS             complement(35985..36872)
                     /locus_tag="OO568_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6640738.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            37029..38069
                     /gene="gpr"
                     /locus_tag="OO568_001314"
     CDS             37029..38069
                     /gene="gpr"
                     /locus_tag="OO568_001314"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6640739.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPVLSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKIRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38109..38603)
                     /locus_tag="OO568_001315"
     CDS             complement(38109..38603)
                     /locus_tag="OO568_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6640740.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38794..39678
                     /gene="yghA"
                     /locus_tag="OO568_001316"
     CDS             38794..39678
                     /gene="yghA"
                     /locus_tag="OO568_001316"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6640741.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     ARLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39950..40375)
                     /gene="exbD"
                     /locus_tag="OO568_001317"
     CDS             complement(39950..40375)
                     /gene="exbD"
                     /locus_tag="OO568_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6640742.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40382..41116)
                     /gene="exbB"
                     /locus_tag="OO568_001318"
     CDS             complement(40382..41116)
                     /gene="exbB"
                     /locus_tag="OO568_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6640743.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41368..42555
                     /gene="metC"
                     /locus_tag="OO568_001319"
     CDS             41368..42555
                     /gene="metC"
                     /locus_tag="OO568_001319"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6640744.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVVRVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42695..43354
                     /locus_tag="OO568_001320"
     CDS             42695..43354
                     /locus_tag="OO568_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6640745.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43394..>44090)
                     /gene="yqhC"
                     /locus_tag="OO568_001321"
     CDS             complement(43394..>44090)
                     /gene="yqhC"
                     /locus_tag="OO568_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6640746.1"
                     /translation="RYDANEYLLLTVPLPFECETYATSEVPLAGLRLNVDILQLQELL
                     MDIGEDEHFQPSMAASGINSATLSEEILFAAERLLDVMERPLDARILGKQIIREILYY
                     VLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEANMSVSAFHHNF
                     KSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSREFKRYFGVTP
                     GEDAARMRAMQGN"
BASE COUNT        10938 a        11755 c        10838 g        10559 t
ORIGIN      
        1 cgtaccgttt aaacaaagac aatacgtctc tttatttctc ataaaacccc aatcccttac
       61 agggcttatt gaaaaattta aaaatcacaa atttctcaat actcgaaatg agatcacata
      121 agaaatcaaa aagccttctc atctatttta agttttccca acggaaaata agagctttca
      181 ttaatactga accactttac tgttctattt gttcaggtat tgcattacgg tgtgctgaga
      241 tccatcggtg catccctgta tcccgcgatg agactaaaat agtcgtgatg taagcttgta
      301 tcagattctt agtgaatacc acatttgtta taattaagtg aaaagataca ttgctgattt
      361 acattatttg gtttacaaaa tgtcatcact tgcagagtta tcgctgacag atacctttaa
      421 tctcttacta ctttcgtgtt attgcaatac aaccatccgt aaagcagggc tatttttagc
      481 aggaaatact caggtgtcaa cattcgaact ccaactctga aatattcata taaattaatg
      541 attttttcat ttcttcgcgc ctaataaaag tattcaaatt cagatgattt ttctgctcaa
      601 cagacacaaa caggcaataa tactctccaa agcgaacaat atttggcatt acgatttaat
      661 gcttcataat caccaaatta tttaaccata aattttctta tttctaacaa aatgattggt
      721 gtgttgttgg agttaaaata catacataat attaacatct gccaacattt cactttccgc
      781 aagacggatg atttcgaatc ctcatccacg ttaccctatc ccaaatatct ttattaatag
      841 gcagtaacga acactgttat agcaagatta acttattaac ctaacggcat cttttatgta
      901 acaagtggag ataaattgct taaaattgat cctcgacaaa tcttaaaagt tctcattatt
      961 ctgcaaaatc agctttgcga ataatcttat gtttttggga gttttgggaa ttttgatcta
     1021 ctcattgttc tagtgaaaaa tataaataag agaaaaaacg ccattcgtca tgaaagcaac
     1081 agatgaagca atgtaataac atactaaaat tgatctcatg aaagtgggta ctttataacc
     1141 agcacagaag ccaagttgta taaatattaa acaattcgca tcgtgaaacg gagactacgt
     1201 ataaagaagc caatcgtaaa aacggttccc tggcgagata aacgacagac aaatggaaag
     1261 ccgggtgata tttaccttca tccggcttta ttgatttacg agactaacat cccggtaaac
     1321 acatacgcct gcagcagggt gataacgccg ataacgctgg caaaaatcag actgtgcttc
     1381 acggtgtagc ggaacagttc agattctcgg cccaccatgc ccgtcgcggc gcaggccacg
     1441 gcgatagatt gcggggagat catcttgcca gttacgccgc cgctggtatt tgctgccacc
     1501 agcagagtgt cagagacgtt gatttgctgc gccgtggtcg attgcagtga accaaacagg
     1561 gcgtttgagg aagtgtccga cccggtaagg aatacgccca gccagccgag aaacggtgag
     1621 aaaaacggga acatcacgcc tgtacctgcc agtaccagcg ccagcgtggt ggacatgccg
     1681 gaatagttgg tgacgaaggc gaatgccagc accatgccaa tcgacaatat cggccacttc
     1741 agactaatta gcgtttcggc aaagacgcca atccctttct tgatgcccac gccgaggatg
     1801 aagatagaga taatcgccgc aataaaaata gcggtgccgc cagccgagag aggttcgaat
     1861 ttaaacaccg catccattgg cgttggttgg gcgacaatgg gtgccgcttt caacacttgt
     1921 tgatgcaaat gagggatctg gaaattaatc accagtgaat aaaacgcgcc gcccggagca
     1981 aataacgctt taaacggctt catggtccag atggtgacca gcaccgttaa gattaaaaac
     2041 ggtgaccacg cacgaatgat ttgccccaga ctatattctg aaggcacggg accgccagaa
     2101 gatggcttat ttaccaccat cgcacctgcg gattgcccca tgctgattgc cgtttcggta
     2161 tttttcggcc gccagacttt aaggaataaa gcgagtgaga cgatactcac cagcgccgaa
     2221 gtaatatccg gcagttccgg accaatatag ttagaggtaa agaactgagt gacagcgaag
     2281 cttcccccag caaccagcgc cgctggccac gtctctttca cccctttcca gccgtccatc
     2341 attgctacca gccagaacgg cacaagaacc gacaggaacg gtaactgacg tcccgccatt
     2401 gcgccaatgt ggaacggatc gattcccgtt acctgaccgg cgaccagaat cggcacgccc
     2461 aacgcaccaa acgccaccgg cgcagtattg gcaatcagac acagccccgc cgcgtataac
     2521 ggtttgaagc ccaggcccac cagcagcgca ccggtaatcg ccaccggcgc accaaagcca
     2581 gccgctcctt ccagcaacgc accaaaggag aaaccaatca gtaacacctg caaacgctga
     2641 tcgtcggtga tggagataac cgagctgcgg ataatatcga actgcccgct ggcaacggtt
     2701 aatttataca ggaacaccgc cgcgacaata atccacgcaa ttggccataa tccataaata
     2761 aagccatagc ccgcagcagc aaatgccata tcaatcggca ttttaaaggc gaatatcgca
     2821 atcaggatag ataatataag ggttattgct ccagcgacat gtcctttcag acgtaatacc
     2881 gcgagtgcaa cgaagaagaa tattatcggg atcagggcga ccagagcgga tagccccagt
     2941 cctcccatcg gcatatacat ttgggtccag gtaaccatat tgttcagtct cttattatct
     3001 ttatatgctt gatatactta aggttgtaat aagcaaaaga ggactgaact gtaaaatata
     3061 ggcgttatac tttacagcaa cagtacgccg ctaacgcaat tgctacctct ggcataacaa
     3121 gtatatcggg taagggtttc tgttccgcac acgcaggcgc agagtatcgt taagatgtcc
     3181 atattgttgt tttaggcccg ctagtaatgc gctacgggta tttaatattg ttaaaccctg
     3241 ataatcgctc cggttatttc cgggataaat gtactaccgc agttactatc atagccccga
     3301 caataaaact tgccggggct tttttgacgc tattaatgac tttctttttc gcgtaaacgc
     3361 caggcgtgta ataacggttc ggtataaccg tttggctgtt tcacgccgag gaagattaaa
     3421 tcgctggcag ctttaaaagc acacgagtta gcgaaattcc ccgccatcgg acgataagcc
     3481 ggatcgccag cattttgctg atcaaccact ttcgccatat tctccagcga cgcctgcacc
     3541 tgttctttgg tcagaatacc gtgacgtaac cagttggcga tatgctggct ggagatacgc
     3601 agcgttgcgc ggtcttccat cagcgccacg ttgtgaatat ccggcacttt tgaacaacca
     3661 atcccctgct ccacccaacg caccacgtac cccagaatcc cctgcacgtt gttatccagc
     3721 tcttgttgga tctcttgcgc cgaccagtta gcgttttcag ccaccggaat agtcagcaga
     3781 tcgtccagca gcggttcaaa ttcagcattg aactcggtct gggcaatgtt ggcttgcacg
     3841 ctctgtacgt tggtttggtg gtagtgcagc gcatggagcg tagcagcggt tggtgacgga
     3901 acccaggctg tgtttgcccc ggcacgcagt tggtcgccct tctggctgta catgtctgcc
     3961 atcaggtccg gcattgccca catgccttta ccgatttgcg ctttaccgcg cagcccacag
     4021 aacagaccgg aaagcacgtt attacgttcg taggctttga tccacggcgt cgatttcatc
     4081 tggtttttac gcagcatcgg gccagcttcc atcaccgaat gcatttcatc gccggtacgg
     4141 tcgaggaaac cggtattgat gaacgccacg cggttgcgcg cctgagcgat acagctccgc
     4201 aagttcagcg aggtccggcg ttcttcatcc ataatgccca ttttcagggt attcggtacc
     4261 ataccgagca ttgtctcaat gcgggtaaac agtttgttgg cgaacgccac ttcctgcgga
     4321 ccgtgcattt tcggtttcac aatatagacg ctgccagtgc gcgagttttt ctgcactttt
     4381 aaatcataga gggcaatcgc gccagtcatg acgccatcaa gaataccttc cgggatttca
     4441 ttgccttcgc tgtcccaaat cacaggaatg gtcatcaaat gacccacgtt gcggataaac
     4501 agcaacgagc gtccgtgcag agaaatttca gagccatcgg cggcggtgta ctgacgatcg
     4561 tcattcagtt tacgtacgat ttgccgaccg tttttctcca ttttctcttg cagagtcccc
     4621 tgcatcaggc ccagcagatt gcggtacagc aggattttat cttccgcatc aaccgccgcg
     4681 accgaatctt cgcagtcgag aatggtactg atagcagctt cgacgataac atcgttgatg
     4741 tgcgccggat cgtctttgcc aatccgcccg ttggcatcaa tttgcagttc aatatgcagg
     4801 ccgttatttt tcagcaaaat gcaggtcggc gcagcggcat cgccacggta accgacaaac
     4861 tgtgctggag tacgtaacgt ggtttcttta ccatttttca actggatgcg taattgttta
     4921 tcgaccacct taaacgccac cacatcctga tagctgccgt tttccagcgg tagtgattca
     4981 tcgaggaaac gtcgaaccca ggcgataacc tgctcaccgc gctgcggatc gtagccactg
     5041 accatcgccc cttcctgcgg gatgatgtcg ctgccgtata acgcatcgta cagtgagccc
     5101 cagcgagcgt tcgccgcgtt cagcgcgtag cgggcgttca ttgccggaac caccagctgt
     5161 ggccccgcct gactggtgat ttcgctgtca atgcccgtgg tttccaccgt cacgcgctcc
     5221 ggttgcggca ccaggtagcc cagttcacaa aggaaagatt tataggccgc tttatctttt
     5281 accggccccg gattgctgcg atgccactca tcaagcgccg cctgaatgcg atcgcgttct
     5341 gccagcaact gacgattttc tggtgccaga tcgtgaacga tctcatcaac attgtgccag
     5401 aatgcagcag cgtccagttc cacgcccggc aaaacttctc catccacaaa acgtttaaaa
     5461 ttggcgtcaa tgcgtaaacg gccctgggtt atggtttgac tcattgtttc tctcctcgtt
     5521 ttcgcttatt tcgccaacac cgctgctgcc gctttcgcga cctgcgcatc ctgtgctccg
     5581 gttaaaccag aaacgcccac ggcaccaata atttgcccat caacaacaac cggtacgccg
     5641 ccttccagcg acgtcagtaa cggcgcagtc acgaacgcgg tacgtccgtt gttcaccatc
     5701 tcttcatagc ccttagtttc acgacgcccc agcgcggcgg tacgcgcttt ctcctgggag
     5761 atgtaagccg caatcggcgc gcaatcgtcc atgcgactta acgccagcag atgaccgccg
     5821 tcatcggcaa cagcaatgga aacagaccag ttatttttct gcgcctcttc ctgacctgcg
     5881 gcaataattg cactcgccat ttgctggcta agaatgactt tagttttcat ttttttattc
     5941 cttttcaagg gcttgttata caatttcaat ccagtgacgc acagaggtac gaccggcgct
     6001 cgccagatgc gtctggcaac caatgttggc ggtgacgatc atttccggtt tgccgctttc
     6061 cagcgcattc attttgttat cccgcagctg gcgtgccaga tcgggatgcg ttaacgcata
     6121 tgttcccgct gaaccgcagc acagatggct gtcgggaacg tccgttaagg taaatccaag
     6181 acgaagtaac acgttttcca cttcgccgtt cagcttttgc gcatgttgta gggtacacgg
     6241 gcagtggaag gccagttttt tatcgccgcg aattgccagt ttttccagcg gttcctcgcg
     6301 cagaagttcg actaaatcga ccgccagttc actgacctga cgcgctttat cggcatataa
     6361 cgcatcgttt ttcagcatct gcccatactc tttgacgaac gcgccgcaac cgctggcggt
     6421 ttgcaaaatt gcctcggcac ctgcttcaat cgcgggccac caggcatcaa tattattgcg
     6481 cgcccgtgcc aaccctttct cctgcgcatt aagatgatag tccaccgcgc cacaacagcc
     6541 tgcttcgtta gctgacatga cgctgatccc cagacgatcc agcactcgcg cagttgccgc
     6601 gttggtgttg ggcgaaagcg taggctgggc gcagccttcc agcattaaaa cccgacgctt
     6661 atggcgcagc ggcggacgcg gtttagcttt caccgtttca gcaggcagtt ttgctctgac
     6721 ctgttccggt aaaaacggtc gcagcaccag ccctacctgc gtcagcgcac ggaagaccgc
     6781 cggacgcggc actacctggc gcaatccttc gcgcagcatt cgctccggca gtgggcgttt
     6841 cactttctgc tcgacaatat cacgcccgat atccagcaaa ttgtgatagc gcacaccaga
     6901 aggacaggtg gtttcacaat tgcggcaagt gaggcagcga tcgagatgct cctgtgtttt
     6961 aagcgtgact tcgttgcctt ccagcacctg tttaatcaga tagatgcgcc cgcgcggccc
     7021 gtccagttca tcgcccagaa gctgataggt tgggcaggtt gcggtacaaa atccgcagtg
     7081 aacacaggcg cgcaggatgc tgtcggcttc cagcgcgcgc gcgttctgcc gcatctcttc
     7141 ggttaatcgg gtttgcatag cctgctcctc aaagttccgc gtacatgcga ccggggttaa
     7201 acacgccgca agggtcgagc tgctgtttaa gctgctggtg atagcggaat aaaggagccg
     7261 atagcggggc aaagccacca tctccggcac taaagcgggt cgcatgaccg ccagcgttgc
     7321 gggcgatgcg atggatttga ttgtcatcgg ctgtcgattt cagccagcgt aacgccccgc
     7381 cccagtcgat cagttgctcg ccgggtaaat ccatcatcgg cgcatcgctg ggtaatgaaa
     7441 tgcgccataa ggtacctggt aacgagaaga acggcagttg ttgttcacgc aattgctgcc
     7501 agaactgacc cgcaacctct tcgccaccca gcagttcacg cgctgctttt accgatcctt
     7561 cgccgccctc aaggcggatc cacaacgcat tgtcgaagta acataagcca ctaatgggta
     7621 atggctggag ttgccactcg gcgatttcac tcatggcttc ttgcaggctg atttcccgac
     7681 gcaggctcag agaggcacgc ggtcgcggta acactttcat tgagatttca gtgagcacgc
     7741 caagacaacc gtagcttccg gccattaacc gtgagagatc gtatccggca acgtttttca
     7801 tcacttcgcc accaaaacgc agatgttttc cagtgccggt aatgatgcgc gtgccgagga
     7861 caaaatcgcg gaccgaaccg ctccacgggc gacgcggccc cgccagcccg caggcgacca
     7921 tcccgcccca ggtggcttct tcaccataat gcggcggctc acagggaagc atttgcccgg
     7981 cgctctccag cgccgcttca atcgccacca gcggcgttcc ggcacgagcg gttatcacca
     8041 gctcggtcgg gtcgtaatta acaatgccgc gatgacaacg cacatccagc gtttgcccgg
     8101 tgactgggcg acctaaaaag gctttgctat tgctgccctg aatcaccagc ggcgttttat
     8161 cgctaattgc ctgattcacc tgctccagca gcgcctgact gtaatcacac tcgcgtagca
     8221 tcaaaagcgc tccagttcag gaaaaggtaa atgaccgtga tgcacatgca tggcaccaaa
     8281 ttcagcacag cggtgtagcg tgggaatgtt tttcccaggg ttcagcaaac catcggggtc
     8341 aaacgccgcc ttgaccgcat ggaaggtcgt gatttcatcg ctgttgaact gggcgcacat
     8401 ttgattgatt ttttctcgcc cgatgccatg ttccccactg atgctgccgc caacttcaac
     8461 gcagagttcg aggatcttcc cgcccagctc ttccgcgcgg gcaaattcac caggttcgtt
     8521 ggcatcgaaa aggattaacg ggtgcatgtt gccatctccg gcatgaaaga cgttggcaac
     8581 acgtaaatca tattgctgcg ataaacgggc aatgccttcc agtacgccag gcagggcgcg
     8641 acgcgggatg gtgccatcca tgcagtagta atccggggag atacgtccta ccgccgggaa
     8701 cgcatttttg cgaccggccc agaaacgtac gcgctctgct tcgtcctgtg ccaaacggac
     8761 gtcagtcgcg cccgctttca acaagatgta gttaacccgc tcgcagtctt cctgtacgtc
     8821 agactccacg ccgtccagtt cgcataacaa aatcgcttcg gcgtcgacgg gataaccggc
     8881 atgaataaaa tcttccgccg cgcggatcga caggttatcc atcatctcca gcccgccggg
     8941 gataatgcca ttggcgatga tgtcaccaac cgcaagtccg gctttttcta ccgagtcaaa
     9001 gctggctaac agaacccgcg ccacgggcgg cttcggcagc agttttaccg tcacttcggt
     9061 ggtcacgccg agcatacctt ccgatccggt gaacagcgcc agcaggtcaa aaccaggtga
     9121 atccagcgcg tccgatccca gcgtcagcgc ctcgccgtcc agcgtttgca cttcaatttt
     9181 cagcaggtta tgtacggtca gaccatattt caggcagtgg acgccaccgg cattttccgc
     9241 cacattgccg ccaattgaac aggcgatttg tgaggaagga tccggcgcgt aatagagatt
     9301 atgcggcgca acggcctggg agatcgccag gttacatacg cccggctgca cgcgcgcgcg
     9361 gcgaccaacg gggttaatgt cgaggctctc tttaaagcgc gccatcacca acaacacacc
     9421 tttttccagc ggcagcgcgc caccagaaag cccggtgcct gcaccacggg tcaccaccgg
     9481 tacacgcagg cgatggcaga cagccagaat cgctgtcacc tgttccattt gcttaggcag
     9541 aacaaccagt aatggacgcg tgcgatacgc gctcaacccg tcacactcgt aaggaatgat
     9601 ctcctcatcg gtatgcagga tctcaagtcc agggacatgc tcacgcagtg ccatcagtac
     9661 cgatgtgcgg tcgacatcgg gtaaagcgcc atcaagacgc tcttcgtaca agatgctcat
     9721 gagtaggctt cgctttgttg tgtggcagct gatttttgcg cgctgcttct gtgaacagtt
     9781 attaagcggg cttttcgttt tcgtctatct ctttagctac cggtcagacc attttttttc
     9841 cagctctgtg accttgtctt ggttaactta atgttaaatt gatgtaacat aatcacttgc
     9901 gtgatgtgcg tgttttgcga gttaagaaca gaaaaattgg tcctacctgt gcacgaggtc
     9961 cgggaatgaa agatgaacgt cgccctattt gcgaagtggt tgcagagagt atcgaacggt
    10021 taattatcga cggcgtactg aaggtcggtc agccgcttcc ctcggaacgt cgactgtgtg
    10081 aaaagctcgg cttctcacgc tccgcactgc gtgaagggct gaccgtgctg cgcgggcgcg
    10141 ggattattga aacggcgcag ggtcgcgatt ctcatgtcgc acggcttaat cgggtgcagg
    10201 acaccagccc gctgatccat ctgttcagta cgcagccgcg aacgctgtac gatctgctcg
    10261 acgttcgcgc attactggag ggcgaatcgg caaggctggc ggcaacgctg ggaacgcagg
    10321 ctgattttgt tgtgataacc cgctgttatg aaaaaatgct cgccgccagt gagaaccaca
    10381 aagagatttc gctgatcgaa catgcgcagt tggatcacgc tttccatctc gccatttgtc
    10441 aggcttctca caatcaggtg ctggtgttta cgctgcaatc attgaccgat ctgatgttta
    10501 attcagtgtt tgccagcgta aataatctct accatcgacc acagcaaaaa aagcagatcg
    10561 atcgccagca tgcgcggatc tacaacgcgg tgttgcagcg gctgccgcac gtcgcgcagc
    10621 gcgcagcgcg cgatcatgta cggaccgtga aaaagaatct ccacgatatc gagctggaag
    10681 gccaccattt gattcgctcg gcggtgccgc tggagatgaa caaggtggga atgtgagcag
    10741 agttttaccc tatccccaag gcgaggggac cgcccctgca cttaacagga tctggtgtta
    10801 aatctgttca tctataccct ctcccgaatg accgcccctg cgctaaacag gatctggtat
    10861 taaatttgct tttcatacaa tcggtaacgc ttgtacggct ccgccccaat gcgttccagc
    10921 atgttattca tgcctgtatt ggtttcgagg atccattaca tctccagcgc atcgatcttc
    10981 cggcgggcaa acggatcgcg taaggcttca atcaataaca gcgcaatcac cgggccgatg
    11041 cggctgaact gatactcgtc gcgcacgccg atcagcggta ctcgcgcagt tcgcacaccg
    11101 ctgactttca aacgccacag caattttgcc cagccgaagg gaaagagcga tccgttcaga
    11161 tcggcaatcg cctcgttgat gttaggcaag ccgacaataa acgcgcaggg tgcagaatca
    11221 atctcagcga tgtagatcat atcgtccggc accagatatt taagttggtc gcccatggtc
    11281 gcgaattcat gttcggtaaa cggcacaaat ccccagttgt gctgccagcc agagttgaaa
    11341 atctcacgca ggatctgcat ctcttcggca aaccgctgac gattgatgca gcgaatggtc
    11401 acctttttgc gcacctgatc catccgtttt tttagcgccg gagagaaagt gagatcggtt
    11461 cgctgcatcc accacgccag taaatcaatg cctttgtgat aacccagttg ttcaatatgc
    11521 gcggcatacc acggtttgcc gtgtggcatc atcgcacagg gtggtgtgtc aaaaccttca
    11581 atcagtaatc cgctttcctg attgatattc aggctgaaag gcccgcagat cttacttgca
    11641 ccttgtgact tcaaccacgc ttccgctgcg ccaaacaacg cggcaaaatc ctgcggatca
    11701 tcaatggcgt caatcatgcc gaaatgaccg gtatcttcac cgtaacgctc acggtgcaag
    11761 gtatctattt gcgcggtaat acgcccaact atctgccccg ctttttttgc cacccacgcc
    11821 tgccagatga tatggtccgt ccccggattt ttcgcagaca aatgctcgtt gcgttcaatg
    11881 aataaagggg gtatccagtt tggatcgtcg ggataaagtg atgacggaaa agcgataaat
    11941 gccttaaggt catttttatt aattactttt tcaattttaa tcatcagcag atcgcattcc
    12001 atatctctgt ggataacaaa taattaatgg tgaaaaacag gttgcaacag caacgagcgg
    12061 catcttaata atcaactcaa tgaatacaaa cgacgaataa atagtattaa ttaatgatta
    12121 tgataaggtc agaggctaca cagaaacgcc tcaatgcctt ttgtcgattt aaggcgaccg
    12181 gacaaggctt cttcttaata tattcataaa ttaaatatca ttcataataa aaataaaaaa
    12241 ctcaaactca ttatttaaat caatgttcat tttcacaccg atgaaaatac tatctcattt
    12301 atcttttatg cctatataat ccaccgcatt attgtcattt tatttttgat atccaatttt
    12361 ttcgtggtgt atatgtctaa taaaatcttt acgcattccc tacctatgcg ctatgccgat
    12421 tttccaacgc tggttgatgc tttggactac gccgctctga gtagagccgg aatgaatttt
    12481 tatgacagac gttgccaact tgaagatcaa ctggaatatc agacattaaa aacgcgtgcc
    12541 gaagctggtg cgaagcggtt gttatcgctg aacctgaaaa aaggcgaacg cgtggcgctg
    12601 attgccgaaa cgagtagcgg gtttgtagag gctttttttg cctgtcagta tgctggctta
    12661 gtcgccttcc cgttggcgat tccaatgggc gtcggtcagc gggattcctg gagcgccaaa
    12721 ctccagggtt tactggcaag ttgccagccc gcagccatta tcactggtga tgagtagttg
    12781 ccactggtca atgccgcgac gcataacaac cccgaattac atgttttaag ccacgcttgg
    12841 tttaaggcat taccggaagc cgatgttgcg ctccagcgtc cagttccgaa cgatatcgcc
    12901 tacctccagt acacctccgg cagcacccgt tttccccgtg gcgtcattat cacccatcgc
    12961 gaagtgatgg ctaatctacg tgctataagc cacgatggca ttaaattacg ccctagcgac
    13021 cgctgcgtct cctggcagcc tttctaccat gatatgggac tggtcggctt tctcctgccc
    13081 cccgtcgcca cgcaactttc agtagattat ttgcgcactc aggattttgc catgcgtcct
    13141 ttgcaatggc ttaaattgat cagtaaaaat cgcggcaccg tttccgttgc gccgccgttt
    13201 ggctatgaat tgtgccaacg ccgcgtgaat gaaaaagatc tcgctgaact ggatctttcc
    13261 tactggcgcg tcgctggtat tggtgctgag ccgatctccg cagaacaact ccatcaattc
    13321 gctgaatgtt tccgtcaggt taactttgac aataaaactt tcatgccgtg ctacggactg
    13381 gcagaaaatg cgctggctgt cagcttctct gatgaagcct ccggggttgt ggttaacgaa
    13441 gtggatcgcg acatcctcga atatcagggt aaagccgtcg cgccgggtgc agagacacgc
    13501 gccgtatcga ctttcgtcaa ctgcggcaaa gcgttgccgg aacatggtat tgaaatccgc
    13561 aatgaagcag gtatgccggt cgcggaacgt gtggtaggcc atatttgcat ctccggtccc
    13621 agtctgatga gcggttactt tggcgaccag gtttcgcaag acgagattgc cgcgacgggc
    13681 tggttagaca ccggcgacct cggttatctg ctggacggtt atctgtatgt caccggacgc
    13741 attaaagatc tgattattat tcgtggccgt aatatctggc cgcaggatat tgaatatata
    13801 gcggaacagg aaccggaaat tcattctggc gatgcgattg cttttgttac cgcccaggaa
    13861 aaaatcattt tgcagatcca gtgtcggatc agcgacgaag aacgtcgcgg gcagcttatc
    13921 cacgcgctgg cggcacggat ccaaagcgaa tttggcgtga ccgcggctat cgatctgttg
    13981 ccgccccaca gtattccccg aacgtcctcc ggcaagcctg cccgtgcgga agcgaaaaaa
    14041 cgttatcaga aggcttatgc tgccagtctt aatgtgcagg aatccctggc atgaatcaaa
    14101 ctgtcgcggt gacgggcgct accgggttta tcggtaaata tattatcgat aacctgctcg
    14161 cccgcggctt tcacgttcgc gcattgacgc gtactgcccg cgctcacgtc aatgataatc
    14221 tcacctgggt gcgcggttcg ctggaagata ctcattcact tagcgagctg gttgccggag
    14281 ccagcgtagt ggtccattgc gccgggcaag tgcgcgggca caaagaagag attttcaccc
    14341 gctgtaacgt tgacggcagc ctgcgcctga tgcaagcagc aaaagagagc ggcttttgcc
    14401 aacgttttct gtttatctct tcgctggcgg cgcgccatcc cgagctctcc tggtatgcaa
    14461 aatccaaata cgtcgccgag caacgactgg cagcaatggc ggacgaaatt acgctgggcg
    14521 tttttcgccc gacagccgtg tatggtcccg gcgataaaga gttaaaaccg ctgtttgact
    14581 ggatgctacg cggcctgctg ccacgacttg gtgcgccaga tacacaactc tcttttcttc
    14641 acgtcaccga ttttgcgcaa gcagtgggtc agtggttaag cgccgaaact gtacagacgc
    14701 aaacctatga attatgcgat ggcgtcgccg gtggctatga ctggcaacgc gtacgacagc
    14761 ttgccgccaa agcccgttgt ggttccgtgc gaatggttga cattcctctg ccggtactca
    14821 caggccttgc ggatatcagt accgcgttga gtcgcctggc aggtaaagaa cctatgctga
    14881 cccgctcgaa aattcgtgaa ttaacccacg ccgactggtc ggcaagtaat aaccgtattt
    14941 ctgaagatat caattggttt cccgggatta gcctggaaca cgcattacgc aacgggctat
    15001 tttgattgtc ttgttttaaa ggtgtgttaa cgtttctatg gtaaatcgtg aaatagtaat
    15061 ggattacatc ctttcctgtt tacaggattt ggtcgaaaac ggtgtggaaa ttaagcccga
    15121 cagcgatctc gtcaacgatc ttggcctgga gtcgatcaaa gtgatggatc tcttaatgat
    15181 gctggaagac agattcgata tttccattcc tattaatatt ctgctgaatg tcaaaccccc
    15241 tgcgcaatta atggaaactc tacttccatg gctggagaat aaataatggg gctatacgat
    15301 aaatatgcgc gccttgctgg cgagcgtctg caatttagtg acaacgggtt aacaccgttt
    15361 ggcacctgta ttgatgaagt gtattccgcc accgaaggcc ggatcggcaa taaaaaagtg
    15421 attctggccg ggaccaataa ttatctcggt ttaaccttta atcatgatgc catcagcgaa
    15481 ggtcaggccg cgttagcggc ccaaggcacc gacaccaccg gttctcgtat ggcaaacggc
    15541 agttatgcgc cgcacctggc gttagagaaa gagattgccg agtttttcaa tcgccctacc
    15601 gctatcgttt tttcaaccgg ttataccgct aatttaggcg tcatcagcgc acttgccgac
    15661 cataacgccg tggtgttact ggatgccgac agccacgcca gtatttatga cgcctgttct
    15721 ctcggtggcg cggaaattat tcgtttccgc cataacgatg ctaaagatct ggaacggcgc
    15781 atggttcgcc tcggtgaacg ggcgaaagag gcgattatca tcgttgaggg catctacagc
    15841 atgctcggtg acgttgcgcc gctggcggaa attgttgata tcaaacgtcg tctcggtggt
    15901 tatttgattg tcgatgaagc gcattcgttt gtcgtgttag gcgcaacggg gcgcggactg
    15961 gctgaagctg tcggcgttga agatgatgtg gatattatcg ttggtacttt cagtaaaagt
    16021 ctggcctcga ttggcggttt tgctgtcggt tcggaagcga tggaagtgct gcgctacggc
    16081 agccgcccgt atatttttac cgcctctcct tctccgtcct gcattgcgac cgtgcgctcg
    16141 tcgttaagaa ctattgccac acaaccagag ctgcggcaaa agctgatgga taacgccaac
    16201 catctgtatg acggtctgca aaaactgggt tatgagttaa gttcacatat tagcccggta
    16261 gtgccagtaa taatcggttc gaaagaggaa ggtttacgca tctggcgcga gcttatctcg
    16321 ccgggcgtgt atgtgaatct tatcctgccg cctgccgcgc ctgcaggcat cacgctgctg
    16381 cgctgtagcg taaatgctgc ccacagtcgt gagcaaatcg atgccatcat tcaggcattt
    16441 gctactctga agcagtaatg tgagcccgcc gtggtgtttg cgccacggcg ggtttaatgt
    16501 tgtctgtcat accaatacac caggctcaac gccaccaaca caaacgctac cggtaaaccg
    16561 agcgcggtaa caggtgcgct taacgcaaat agcaatccca gattaaccat gatttgatag
    16621 ctgatccagg tgagtaagcc cacgatgacg cctacagcga gacggctacc catcccaggc
    16681 gaacgcgggg cactaaaggt aaacggcaca gccagcaaaa tcatcgccag ggtcaggatg
    16741 ggttgcccca gtttttccca cagagcaagg cggtattcgc tgctgggttg cccggtttcc
    16801 tgcaaataat ggatgtaatg attaagctga cggacagaaa aagtattccc cggcattgat
    16861 aactcttcaa gattcatact ggtgaaggcc gattgccagg caagattatc taatgtttcc
    16921 agcgtttctt tgccattcag ccatttttta tgattcacac catgcaggac ccacgttttg
    16981 tcgtcttcaa tggtggcact gcgtgcataa atgtaggatt caagcgagag atcgtcgcga
    17041 taatgaaata tctccacgcc caccggctgg ccctgctcgt tcagcgattt cactgtcaca
    17101 aattcattac cacgcctggc ccacagcata ttgccggtaa tgtcatcgtc ctcccccaac
    17161 gcggtggcgg tcgatttgat ttgcaacgcc tgttgctgca atggcgacgc cacccactca
    17221 tctatcgcgc ctaacgaaac agtccacaat atcccggcaa ccagcgccac cagtgcaata
    17281 cggaagatag aaaaccccgt gctgcgaatg gcggtaagct cactgttttt cgataactgc
    17341 cccagtccaa caataccacc caataacgcg ataaacggcg aaagttcgac cagtgtgcgc
    17401 ggtaaggtca ttagcaccac cagcaccgcc tgagtccagc gataaccgcc cgggctgaca
    17461 ccatccagtt cgttaatcag gttgaaggtg gtaaaaagcg gcagcaatag ccctgcagcg
    17521 gcggcaaaac cgagaaagag atgacggatt aaatagcgac tgaaaacatt catcgggaaa
    17581 atttctgcag caaagagaag tcacgtgcga ccagtatcag caggcccatt agcatgagtc
    17641 cgggcactaa ccacaaacca ggaatgaggg gaatcgcgcc gttagccacc agtgtacggc
    17701 agacgtcgcc gccataaaaa atggcaataa acagcaacgt cagtggcaat aacgttgaaa
    17761 atcgcccttg ccgcggtttt acccgactta atgaaatggc cagcagcgcc atcaacaatg
    17821 cggtcaggcc acggctttgt cgccattgca gttcggcatg atcggcagga aaggcggagc
    17881 gcgccagctc cgtgaccgat ttcgctttgc gtttgacgtt cgggctttga tccagcggat
    17941 tcagatgtaa ttgcagatta cggtagatct gttcgttgtc gtcacgcccc tgatgatcga
    18001 gaagataggc agtgccgttg tgcaacataa cggtcggttt ctcgggtgat gggtcaacca
    18061 catcaaccga acgggcgcgg aaaatacggg ttcgattggc agtagaagta taaatcagcg
    18121 cgtcagtcag gcgattatta tcctgatcaa ccgtctgcga aaggatcatt cgtccgttat
    18181 cgttagtgtt aaatttcttt gcccgcaact ggcgaacatc cagctccgac tgcgactgct
    18241 gctccagttg ataaatctgc gcataagccc acggacgacc atacatcgac agaagggtga
    18301 caaaaatact tagcggtatc gccagataaa gaacggcttt gtacaagcgt cccggactgc
    18361 cccccgccgc agagatagcg gtaatttccg agtcggtata catctgccct agcgtcacgc
    18421 caactgacac atacagacca acaggtaaca acatctccag tgcaatcagt actttgtaga
    18481 aaacgatatc cagcacaaca tcaagagcta atgtcccatt tgccgcttcg gtcagataac
    18541 gctgtgcgga gtagctggcg aaaataaaga tcagaaaacc gacgataatc agcaccagac
    18601 ggcgcgtacc acgcatgatg tagtgttcaa ccagcttcat ctcactaacg ccctcaatcc
    18661 tttgagtttt ttattcattg atcagtttct gcgccaacgc aatgacatga tctacctctt
    18721 ccggcgtcag gctgccgtcc tttgcccact gaatttgccc ggctttattc agcaccagaa
    18781 tggttgaact ctgctccggc aagcgccagg caacgcgtcc ctgtccattg ccgtcaataa
    18841 cgaattgcgc ccacggatag cggcgtttat ttttttcgat tttgccaaca acaaaatagc
    18901 cagtaccaaa gatggcatca tcggtgttca caatggtggt gggctgaaaa cgatcctgcg
    18961 gaaagtttgc cgcttcaacc gctttaatta acagtgagtt ctttttcttc gccgacttac
    19021 gcccggcgat atactgaata atgcggactt ttcccgctaa ttgtgtgctg ttccactttt
    19081 ggtagtgaat ttcgtcgtcg ctattcagaa gcagctcgcc gccttcctgg atataaacag
    19141 gggtaaccgt tttgccagtg acgaaattgt gtgcctgcac acccggggct aagagcatga
    19201 taatcaacgc aataatcaaa cgagatgaca tcattgctcc ggctatagcg gaaaacgcga
    19261 aatcagcagc tctaagcgct gacaaatatc gctatccagc gccaacggtg cataatacga
    19321 ctggcggttt tccatatcat ccgccaccat gtcgtcgtgt ttatctccca tcaggccatt
    19381 aatggcggcg aggaaacggg aaatctgtaa cgcctcttta ttttcaggat caagcgatct
    19441 taaggtgtcg taataagaaa cctgctggct gatgattttt cgcacatcat gaaattccct
    19501 gccggttagt cgcggtttgg caataatctc tttcagggta tgcatttgct tttgccgata
    19561 ggcaataaaa ctgcttacgc tttctaactg gctatggcgt aatgaatagt tcaccaacga
    19621 ccagaccgga gagcttaaat aaatccgtaa caggttgccg taatacgaca caatattttt
    19681 cttaccgtta cgaaagccgt cctgaaaacg ccccagaaaa acggtcgttt tgccgaataa
    19741 aaatccggcc tgatgcttct ttaaatacag ccgttgcgca aaacgcaggt gcttgtagcg
    19801 cgctcgcatg tatttaaatt gtttgcgctc atcagcagtc agctcatcgc ctttggcttg
    19861 tttgcgaact aaacgcagta attcctgacg atcaacgcca ttgagccaga attgcaggca
    19921 taacgcgtaa ttatcatgaa tgatttcggt ggtacaactt agtgcaatag tcggcggcaa
    19981 actaacctgt gcatcaacga catcatcttc ttcaatcgca acaaatagac gctcgctcgc
    20041 ctcaagcggg aaatattttc tcatcatcag gatttctctc cggctgcgtt ataaagcaag
    20101 gaatggcgct gaaaaaatgc ctttaaggtt ggaattaacg agagcgccac gtccagacat
    20161 tgcccaccga agtaagcaaa cgctgctccc agcacaccaa acagatgaca gaacatgaac
    20221 agcaatacga tgtagccgat tgaggcgatg gtttgcgcca cgagaaaagc gcgctgtttt
    20281 cccgccatca atagcaatga ctcctgcggg aagcccagca tcgaaatcac aattgcgccc
    20341 agcatcacct gaatcaggtc atacgcttcg agatacttaa cgccaaacac cagcgaaatg
    20401 agcggcttgc cgacgatcaa caccgcgagc gccaccagaa taccgattcc gcccgccagt
    20461 aaaccggact tcacacctaa caaccacggt ctggtggtgc gcggatctaa acgcatcacc
    20521 tctgggtaaa aacttttacc cagcaaaccc gctggcgttc cggcggcgtc gaagaatgtc
    20581 atggcgattt taaataaccc agcggcagcg ggtcctaaca cgatccccac caacactgtg
    20641 ctgcacgagt tacgcgccga ccagatggag tgggcaatgt tggttgacca gacaaaactc
    20701 cacgcgcctt taatgtgtcg ggcagactca aacagattca atttgaaggc gttatggata
    20761 tttctacggc gtaattcgcg cgcggcaaac caccagtaca tggtgccgcc aaccagattc
    20821 gacacgtacc aggcaataac aaaacccgca aaaccaaagt caaaatacca ggctgcgacg
    20881 ctccccgctg cgcgcagaaa aggtttcgtc gcctgctgta cagcaatcaa atcaaagcga
    20941 tctaccgcac gcagaatgcc ggtcggcgtg gaggaagcca ttgaaggaat gagcgtgcaa
    21001 tacagcgccg ccagccaaaa gctttggtca tccagaccta atgaatggga aaggaatggc
    21061 agtaaggcaa tgccgccgac aatcgccacc gcgccgctga cgatatccag cgagaaggaa
    21121 aatgagacga cattgcgaaa ttgctgcgga ttattgttgg tcaaggctgg cgttccgtac
    21181 tgaaccacca gctgccatgt ctgaaactta ataaaatcgc tgatcgactt ggcgtacgat
    21241 tgcacaatca ccagtacacc aaacatggcg ggcgtcatcc ctttaccggc acacgagagc
    21301 gccaacagcc ccagcaaggc gctctcgaca ttactggagc ctaaccaggc gctattgcga
    21361 ataatggtgc gaaacgcgcc atctgcaaac caatgtttga tgttaaaacc cgccaattca
    21421 gcctgacctt atcgttgata gtaaaaaata tcccgccagc cttaagttaa acttcggcgg
    21481 tcagaaacga tggcaaccag agaaaccgcc ttctgtacct gttccagcac ttcgctgtag
    21541 ggcgctctgg aatcaatctc aagaattttt gtgccgttat agccaatcgt cgacatgacg
    21601 ccgattttgt cctgcagctc ggcatactca tggtcaggct tgcgggagat ggcagtatca
    21661 atatcaatcc ccaaacgaat gattaattca ggacggtatt gcgccatttt ttggtataaa
    21721 ctccgttcgc gctgcgccag aaacatgctg attttcccgg tcgcacgttc gacgccgatc
    21781 cccggtccgt cataataaaa gcccgaaatt tcagcctgcg ggaagcgatc gctgaccacc
    21841 agaacgccac tttgcgccag tcgctgaacc ttgcgtagat tcgccattct tcgcagcgag
    21901 aagcagtaca taatcaccgc cgcccatagc gccggagatt tggttttcat gctttgggtt
    21961 ttcgaggatt tggccgccag tcgccgttcc agccagacgc caaccaacgg caatcgtttg
    22021 attttgtcgc cgtcttcgcc ggagagcagc cccagatagc ggcgctcggt ttgccagtgt
    22081 tgttgcagcg atttcaccag gtcggtggtc agcgtggatt taccggtgcc atcacacccg
    22141 accaccgcaa tcagccccgg aatgtagttg ggctgcggcg cggttgtact attaacagtt
    22201 tgtgtttgta gtgcatccat tcgttaaatc atcccctatc cattttggtg atcaggaaag
    22261 cgcggcgtga attgcgcgta gtgacgcttg cataatttca tcggctggtt gccgaccatc
    22321 cagttcaagg atcttggcgc cattgaatgt cagttgcggc gtaacggcga ttttttcctg
    22381 taacgctgcc agttggtggt caggtttacg cgcaaacgcg gtttgttcat caatgccaag
    22441 acgaatcaac aatacgggta aataagacgc catccattgg tacagcttca actcgcgctg
    22501 ccttaacatt tttatccaac cgttaccgcc cgtggttttt gccaattgcg ggccatcaaa
    22561 gcgaaaaccc ggcacttcaa cctgcgggta gcggtcggtg atgagcagat agccttgctg
    22621 gcttttacac aacattttgc gaaacttgta cgcccgccag caggaaagca gatagataac
    22681 cagcgcagta atattgccag gcggtgttga gggcttttcg tgtacatgtg ccgctttact
    22741 tcgcagataa cacccacaag gtgcgccaat aacagggagc tgtgaaatcc attcgccaat
    22801 tcgcccggac gattgcccga gataaatgtg ttctgttggc attcttgctg ccagttcatt
    22861 taccaggctt gccgtgaggg tcgatttacc tgaaccatca catcctacaa tggcaataac
    22921 acgcactggc gtggaattaa ttattgacat attacgttga ttcacgaaaa acccggcagt
    22981 aaataatgta ttgaatatta cgtggtccgt gctcagaata tccgttcaac cttattgtga
    23041 caatggttaa ttgtgacatt gacctgaatt acgctttcat aaaaacatat taaccaaata
    23101 aatattttta atggatattt aaattaaagg atatattcat gcagtcaata acacctccat
    23161 taattgccgt tattggtagc gatggttcag gcaagtcaac ggtgtgtgaa catcttatta
    23221 ccgttgtcga aaaatatggt gctgccgaaa gagttcattt aggaaaacag gccggaaatg
    23281 tcggtcgtgc agtgacaaaa ttacctttga tgggaaaatc cttacataaa acaattgaac
    23341 gaaatcaggt gaaaacagca aaaaaattgc ctggaccagt tccggcgctg gtaattacag
    23401 cgtttgtcgc ccgtcgctta ctgcgctttc gtcatatgct tgcctgtcgt cgtcgcgggt
    23461 taattgttct aaccgaccgt tatcctcagg accaaattcc tggcgcttac gatggtacgg
    23521 tgttcccacc taacgttgaa ggtggtcgtt ttgtctcatg gctggcaagc caggaacgta
    23581 aagcgtttca ctggatggcg agccataagc ctgatctggt catcaaactc aatgttgacc
    23641 ttgaagttgc ctgtgcacgt aaacccgacc ataaacggga atcgctggcg aggaagattg
    23701 ccataacgcc acagttaacc tttggtggtg cacaactggt tgatatcgat gccaatcagc
    23761 cactggaaca ggtgttggtt gatgcagaaa aagcgattac ggattttatg accgcgcgtg
    23821 gttatcacta gtcaaaaatg gaaatgcccg atcgccagga ccgggcattt tcaggaaggt
    23881 taaatcaact gcaatgctat ccagtacagc ccaccagaaa gaaaaattgc cgccggtaaa
    23941 gtaaacaccc acgccatcag gatgctggtt accgttttac gctgtaaccc accgccgtcc
    24001 accaccatcg tccctgctac cgcagacgag aggacgtgtg ttgtggagac aggcatccca
    24061 atataactgg caaggccgat agacactgcc gccgtcattt gtgccgccat gccttgcgca
    24121 tacgtcatgc cgcgcttacc aatcttctca ccgatggtca tcgctacacg acgccagcca
    24181 atcatggtgc caatgccgag cgccagtgct accgccatga tgatccacac cggagcgtac
    24241 tcaatggtgc ttaacatatc gctgcgcagt tttttcagca ggttctggtc ttctttactg
    24301 acgcctggca gtttcgccag cttcgcggag gtatcagaga tgcacagcat aatgcggcgc
    24361 agctggctgc gctgactcac gcttaacggc tcgtaacttt ccatattgcc tggcagcatc
    24421 gttttaacgc gcgcaatcgc atcaaaggta tttgccggat gacagtgaaa ctctgttact
    24481 tgcgtgccat cagtcgatgc tgcaggcaat ggaggttcca tcgcaatcaa cttctgcggc
    24541 agttcaggat gctgttgcag gtagtgttcg aagttggtaa cggcatcgcg ggtacgggta
    24601 atttcatagc cggacgcatt catattgacg acgaagccag caggggcaat ccccaccagt
    24661 accagcatta ccaggccgat ccctttctgc ccgtcgttca cgccgtgcga aaacgccacg
    24721 cctgcagcgg aaacaatcag cgcaatacgc gtccagaatg gcggtttacg tttgcccttt
    24781 ttctttttgc gatcttccgg aatgcggtga atacggtcac gctttttcgt cccactccag
    24841 aaacggcgca gcaggaaaat cagaccaccc gcaatgacca ggccgacgat aggggaaaca
    24901 atcagcgagg agaaaatttt ggttacttca cgcaggttta acgcatccat caccgatgag
    24961 ccggttaaca gcgcgttggt taaaccgatg ccgataatcg caccaatcaa ggtgtgcgaa
    25021 ctggaggccg gtaaaccaaa gaaccacgtt cccaggttcc agataatcgc cgccagcagc
    25081 atggaaaaga ccatcgccag accgtgagtt gaccccatat tcagcaacaa atcggttggt
    25141 aacatatgga caatggcata ggcaacgcta agtccgccca ataacacgcc aaaaaagtta
    25201 aaaaatgccg ccatcaccac agcaagttgt ggctgcatgg cacgagtata aataacggtt
    25261 gccaccgcat tcgccgtgtc atgaaaacca ttgattgctt cgtagaacaa cacaaatgcc
    25321 agagcaagta ataacaaaag ccccgtatat atatcaaggc caacaaataa atttagcata
    25381 tattagatta cgccattttg aatttacgaa cggacgcatt atcagtgact ttaacggcat
    25441 gggcaaagtg aaatatcatt ttttaacgat aaaaagagtg ttatttcttg tgtattagac
    25501 aataagcatt cattcaaaac ctttagaaat aatcaaaaca caggtattcc actggttaat
    25561 atattcacat atgaaatgaa taaatattgg aatatataaa tattgaatat tttgattaat
    25621 ccccgtacta attattcttc ataatcagta cggggttgct acaacatgta ttactttttc
    25681 accacaatta acggttcaat atcactctct tttttgatga ccagtgattc atcaccgcgc
    25741 aaacacgtcc caccgtagtt gccgccaacg gtgaaggtac atacctgaat gtatttaccg
    25801 tccactttcg gcaaacacca cagttgctga tagatgtttt tctgctcggc aaatttaccg
    25861 ctggttttgt ccagcacctc ttcatgatgg ccgaccaggt cgatattgct accacagcga
    25921 ccggcgatcg gtttcactgc gtaacctgtt ttcaccaatt catcattaac agtgaaatcg
    25981 gtatccagca ggtaacgatg gtgcgggaac agcgaccaga ggatcggcag aatcgctttg
    26041 ttgccgggga tcaccgtcca cagcggctca aagaccagca cttccgggcg cagcaatacg
    26101 tcgataagac gcacttcgtt ttgcggatga ccggtacgga ttggcaccgc agcaaactca
    26161 cggtcgctaa cttcacgaat ctgatcaaac gcggtttccc acgcccaggt tttccacacg
    26221 cagttaacca gtcgcccttc cccatcaatc agttgcccgg cagcatccca gcccagttca
    26281 tccagcccgc gcaagatacg cgtttcaaag cccgcctggt gcagcgcctg ctccataaac
    26341 tgcgcgtgat agttttcctc gatatctttg tcctgcatga tatggacaaa cggacgtgca
    26401 cgactgtgtt tccaggcacc agccagttcg ttaatcagcc cttccgccgg attgaagccg
    26461 ttgcctttat agccctgctc cgcccaacgt tcgaggatca agcccgcttc ggtatgacag
    26521 gaggcggagt cggcgttgta ttcgtaaacc ttcaggccac gctcatccat gcagaaatcc
    26581 atacgaccag tgatcatatg gtgacggcga cgctgccagg agagacgcaa acgtggccag
    26641 aggattttcg gaatgtcgaa cagcgccagc aggttgtcat ctttcagcac cttgtcggtt
    26701 gcgtgaagat acatcagatg cagctcgttg gtggctttaa ttagctcctg ctcggcactc
    26761 tcggtaatgg tgtagtaatg atacggatcc tgattgatca cctgaccgtt ggcctgcaca
    26821 taggcgtttt gtagcggatc tttttcatcc agccatttac cgtcaaactg gcctttgttt
    26881 tccagacgcg ctccactgat tttcagcagc tcgcctgcaa tttccggctg cggtaagctg
    26941 tattcagtat cttccgtctg gatcatccag cccagaatgg tggtgtcatc aaaagtgtct
    27001 ttaagggtat agcagccgtt ttcgaccacc atctccagct cgcgcgtcca ctgttgccct
    27061 tgcggcaacg gggaatgaat cacgttctgt tccgcaatac ggactttgtt gccatgcaat
    27121 tgggtaatga tggcgacatg gccagtgtct ttaaattcac cgcctttatc ccagataaga
    27181 agcgcacccg cgaccggcgc acgcggcgag ccgttaggaa atgcctgcaa tggcaggatg
    27241 ttgtcattaa ccacttctcg caggaagcgc agcgagaaaa tctcccacgc catacccacg
    27301 tcagtaaaga ccacaccgta attcagaaag agaaaacggc gagcaaattc aacgcattgc
    27361 cacttgtggc ccatatattc gtcgtcgata tagctacgga atacggcgtc atcttcgtat
    27421 tcctgcggat cgagagaact gtaatctgaa gagtagattg ctaccccacc tggggcgtag
    27481 cccaataatg tcccgaacgg ggcatcctgg ctggtcgttc ctttgctcat cactttacct
    27541 ttaacaatac aacctaagca gttggcgtaa ttgttgtgct ctgattacct gctcagcgac
    27601 attaaccgga cagaggtcag actaatcata cacctcatcg cagcggctgg cgcaaagggt
    27661 taaaaaattc acattctgta cagcaagtga cctgctacac tgcttcaaca ctaccactca
    27721 gaaggcaact cactatgaca gacaatactt atcagcccgc gaaagtctgg acgtgggata
    27781 aatccgctgg cggcgcgttc gccaatatca atcgcccgtt ttctggtccg acgcatgaaa
    27841 aaacgctgcc cgttggcaaa catccattgc aactttattc gctgggaacg ccgaacggtc
    27901 agaaagtaac gattatgctt gaggagctac tggcgctggg cgttactggt gcagagtacg
    27961 acgcctggct gattcgtatt ggcgatggcg atcaattctc cagcggcttt gtcgaagtga
    28021 acccaaactc gaagatcccg gcgctgcgcg atcatacgca taatccgccg atccgcgtgt
    28081 ttgaatctgg ttcgatcctg ctttatctgg cggagaaatt tggctacttc ctgccgcagg
    28141 atctggcaaa acggactgaa acgttgaact ggctgttctg gttacagggt gccgcgccgt
    28201 tcctcggcgg tggttttggt cacttttacc attacgcgcc ggtgaaaatt gagtacgcca
    28261 tcaaccgctt taccatggaa gccaagcgcc tgctcgacgt gctggataag caactggcgc
    28321 agcataagtt tgttgcgggc aatgagtaca ccattgcgga tatggcgatt tggccgtggt
    28381 ttggcaacgt ggtgctcggc ggcgtgtatg atgccgcaga gtttctggat gcgggaagtt
    28441 ataaacatgt gcaacgctgg gcgaaagaag tgggcgaacg tccggcagtg aaacgtgggc
    28501 gtattgttaa ccgcaccaac gggccgctga acgagcagtt gcatgagcgc catgacgcca
    28561 gtgattttga gacaaataca gaagataagc gtcaggggta agggttggtg ttcgtcgcag
    28621 caagccatcc aggccggata aggcgttcac gccgcatccg gcaatcgtgc acaatgcctg
    28681 atgcgacgct gccgcgtctt atcaggccta caaaagctaa tcatcacgcg ctggtaatgt
    28741 cgtaatccat ttggcgcagt gcgtctaatg tggctttggc ttcatcttcg tcgatgatgc
    28801 tcatggcaaa tccgacgtgt accagcaccc actggcccag cagatcggca gggttacctt
    28861 cgcaaatcag ggcgatattc acgtcgcgct tgataccaca tacttcaacc tgcgcaagct
    28921 ggtgaatatc ttcaccgaca gccagcacct ggcctggaac gccaatacac attgttaact
    28981 ccggttattt cgattgccgg acaaacgcga aaaagaacct ttagcgggtg ttccctctca
    29041 ccctaaccct ctccccggag gggcgagggg accgtccgtg cgctttttag gcgttgtcct
    29101 cagtcttatt caacttcaat acttttgacg atcagcgaat cgccggtatc gacacgcaac
    29161 cgctcgccgt gacagagcgg acactgcgca tcgtgctgat gaatctccac cacctggctg
    29221 caatcccagc accaggcctg ggcgggttta tagacgatat gtaaatcgca cccttgcgcc
    29281 accgttccgt ggcagacaat ttcaaaacta aaacggacgg cgctctcctc aacgcaggag
    29341 agcgcgccaa tttccagcca cacggcggtg acgcgcttaa catcgtgctg ctccgcctgc
    29401 cgttggataa tttcaacggc gctctggcaa agcgacaact catgcatttt cgcccctcct
    29461 gcggccaaac agcagggcgc gacgccctgc gtgtggtaca tccggattag tgactggcaa
    29521 cgaaaggatc attcgtgcgc agtcatccgt caggcgttgg ccttcttcaa tcgacatgct
    29581 gtgcgaaagc ggcgacatca gcgaacagga gaggtattgc gaaacaccgt ccagttcgcc
    29641 aacggtaaag gtcatggtgc catacggcag ttgcagacca attttttcac tcactttgcg
    29701 cagcggccag agttgatccg ggccggggaa gataactgca ctcagcatcc acggggtgat
    29761 cacacacccc gtccactgac cttcgaacag cgtaaaatca gaaacataca ccggcattga
    29821 aggatgcaga aaagagagat cgtgcatcga acgccgggca atttcttcaa acgctgcctg
    29881 tacttgcgcc ttcggggagg tctggaaacc tgctatctct tcagtcatga atcgcctccc
    29941 gtgggatggc ttccacgcct gattcgcgca gcgcagccag aaccagctca agcgcaggtt
    30001 caatcattgc ttcaaccgtc ggcgttaagc cgatgtgtgg ctccagcgat tccgggatca
    30061 cgccgaccag ggtcagcttt ttcggaaact caccggtgaa gcgcagggcc gacaatacgt
    30121 cggccaggcc aagctgatgc ggagagattt tgttggtaaa caacgccgga acttcttcat
    30181 cccgcaggat catcatcgtt cccggcgtgt tctttttcga cacaatggca tccgcaataa
    30241 tcagatgatc gcgatttgcc atgtcgccaa gcagctccat tcccgccgtg ccgccatcga
    30301 ggatctcaac ataatccggc agaatgtatc gttgctctaa cgcttcgaca atccgcacgc
    30361 cgatggcttc atcggtcagc aaaatattgc cgacccctaa gactaaaata cgcattacag
    30421 aaccttcact gaaaccactt cgttgccgtc ggcatccact acgtgtaccg cacaggccat
    30481 gcacgggtcg aaggagtgaa tggtacgcac cacttccagc ggtttattcg gatcggcaac
    30541 cggtgtaccc accagcgact gctcgtaagg accgacgtca tcattgaagt tacgcggacc
    30601 agagttccag gttgatggaa caaccgcctg gtagttgctg atgataccgt ctttaataac
    30661 catccagtga gagagcatac cgcgcggcgc ttcgaggaag ccaacacctt tgaactcacc
    30721 cgttgccgga atgttcggct tcacaaaggt ggtgtgatcg cctttgccga tattggtgat
    30781 cagtgcactg tattggtttt gcaggatatc ctgcaattca cagcagtgaa cggtacgacc
    30841 aataatacgg cccagcgtgg agtgcagctg tgccacttcc agcgtgttgc cagtcagttt
    30901 ctgataaatc gcaacgattt cattcagttt gttctgggta gattcgcgac ctgccgccag
    30961 tttcaccagc atattagcca gcggacccac ttctaccgtt ttgccgtaga aagtcggtga
    31021 tttcacccag gaatatttcc cgtcgtcaga ccaaccatca taagccggaa tggtggtacc
    31081 ttcccatggc gcctgtggcg cttcgtcttt ataccaggag tgcttcgcgc tttcctgaat
    31141 gcctttgatc aggtattcat cggaatgaga agtgatcgga cgatacgagg acagatccgc
    31201 attctcaatg tagccgcccg ggaacaggaa gctgccgttt ttgctgtcgg tcgggaattc
    31261 cggcacgctc aggtagttca ccgcaccttt accgcgcgtc agccattccg ggtagaacgc
    31321 ggcgataacc gcggtatcaa ccttataaac ctgctcaaca aagtcgctca gtttgtcgat
    31381 gaaagacttg atgtacatca ggcgctcaag gttcaacacg cccaacccgt cgaggttgat
    31441 tgggttcgcg acaccactca ctgccaggtt ctgaatgtgc ggcgttttac cgcccagcag
    31501 cgccacgaca cggttagcgt cacgctggca ctccagcgcc tgcaggtagt gcgctaccgc
    31561 aatcaggttc acttccggcg gcagtttcat cgccgggtga ccccagtagc cattggcgaa
    31621 aatacccaac tgaccgctgg caaccagatc tttgatcttg ttctgaactt tagtgaactc
    31681 ttccggactg ttcagatgcc aggtcgaaac gcctttcagc atttcggagg ctttggttgg
    31741 gtcagcttgc agtgcagaag tgatgtccac ccagtccagc gccgaaagct gatagaaatg
    31801 aacgatatgg tcatgcgtgg tgtgcgcagc cagaatgatg ttacggatgt attgcgcgtt
    31861 aaccggaacg tcgatattca gcgcactttc tgccgcacga acggaagaca gcgcgtgagt
    31921 ggtagtacat acgccacaga tacgttgcac aatcatccat gcatcgcgcg gatcgcggtt
    31981 tttcacgatc tcttccatgc cgcgccacat ggtaccggaa gcccatgctt tcgaaacgac
    32041 gccattttcg atttcgcaat cgatgcgtaa atgcccctca atacgggtta ccggatcaat
    32101 agtaattctc tggctcatgc tttgctcgcc tcatgacgat tatgatcgtt ttgttttaaa
    32161 ggaggaagta tcggcagtag acgaatgagt acgatgtaag cgcaaatctc aatagccaca
    32221 aaaccaatag aaatcaacag ttcttcccag gtcgggaagt aggcgtaacc gccgcccggg
    32281 ttgaatgcca ccaacgaata ggtcagacgc caggttgcac aacctaacag tgcgctcagt
    32341 gctgacagga acagcatgcg ggaatcatta cgcagcttcg ccacacgcag aacgaccagc
    32401 gggaagagca tcagcaggac ttcaatccag aacatcacgg agtagaagtc accggcaaac
    32461 gctaacgaca gcttgtcgcg atagataagc tcgccaaagc gcagcccgat gaaaatcgcc
    32521 agcaacacac tgatggtgtt ggtcagcttg acgaacaggc tcttttcatc cggaccgttg
    32581 ccacgcagac ccgcctgcac cagtgaacct tcaaagatga caatcgagaa gcccatgatg
    32641 aacgccgtca gcagcgagaa cagcggcaac atttcatagc tctgccagag cggatgcacc
    32701 ttgtagcccg ccgagatcat cagcgacccc attgaagact ggtgcatggt cggcagcagc
    32761 gcaccgagcg cgatgatgaa gaacatcacc ttgttcagac gttgtagcga caccttccag
    32821 cccagacgtt caaacagtgc cggagcaaac tccagagcca tcacgccgat gtagatggtc
    32881 atacagaccg ccgtctcgaa cagtaccgag ttcacgttga agtgacccgg aatgtagaag
    32941 tacggcaggt tccagtagcg acccacgtcg atagtgatcg acaagccacc cagtgagtaa
    33001 ccaaacagac tcgccaacag cgccggacgc accagcggat ggtattgccc acggttaaag
    33061 acgtataccg cccacgccag cgcccagccg ccacaggcaa agccggtgcc aatcagcagg
    33121 tcaaacgcga tccacacgcc ccacgggaag ccgccgttca ggtcagagac agagcccaga
    33181 ccgaacacca gacgcttcac aatcaggagc atacagatga cgattaacgg tccaaaaatc
    33241 atgaccggtt tactgatgat tttgccgccc agcggttgtg gatcatgact catgatcgtc
    33301 tcctccgtcg tgatggtcgt ttttggtgtt gcgacgaacc agcacggtta agcccgccag
    33361 cacagccagt ggtagcatca tgcctttata cagggtgtgt tgaatatttt cggaacgcgc
    33421 accggtagaa agatcgtcca gtttcggcag gtcgagattt tcataaggca cacccgtcag
    33481 taccagaacc tgagtaccgc cgccctcttt ctcgccgtac agatgcggat aatatttcgg
    33541 caccgtatgc aggtaagtgt cgccagattt cagcgtctga cgcggatagt ggtattcgct
    33601 gccaggcttc agcgccagac gttttttcgc ctccgccatc agctcttcac gcgtaccgaa
    33661 aatcaccgcg cccgccgggc acacttctac gcagccaggc agaccgcctt tatcgagacg
    33721 ttccacacct ttctggttgc acagctcgca cttatgcagc gcaccaaacg ggttgttgta
    33781 gtcgtacttc ggcacgttgt acggacaggc gaccatgcag taacggcaac cggtgcacac
    33841 gtctttgtcg taatggacaa tgccggtttt cggatctttt ttcagtgcag agaccgggca
    33901 cacagagaca cagttcggat cgacgcagtg catacactgt ttcttaatgt acgcgtagcc
    33961 gttctcctcc tggtctttgt tgacccctgt gccgctggtc cacacctgaa tgatgttatt
    34021 ggtatacggc gacagtttgt cgttgttcga ccaggtctgt tccccttgcg ggttacgttc
    34081 agggaagttg atatcctgac acttggtgac gcaagcctgg cagcctacgc acaaggtcga
    34141 gtcgtacaac atccccagcg atcccggaat tggcgggcgg ttttcagcag ccgcatgact
    34201 gacagacggc agcgcgcccg tcagcaatgc cccgcaggag gctgctttaa taaaattacg
    34261 tctgttcacg gttattctcc ccgtgagtca gcgttatctt tcttttgctg acgacccagt
    34321 tcacgcaccg ccatcacgct gacaccggca accagcccaa ccacaccgcc gagcaaacca
    34381 atagcgcctg cagagacgtt gccgccctct ttagcgttaa catccggttt ctgtgaacgc
    34441 ggagtctgat tttcgacgtt ggcaagctga tggatgcctt tatggaagcc gatacctttt
    34501 tcgttacagc catagcacgg gtgaccaatc gccaccggcc acacaccgcc aacatcgcag
    34561 aattgcagcg ttgagcagtt gccgtaagtt tctggccctt tacagccgag gtggtacagg
    34621 caccagcctt cgcggtggcc ttcatcaccg aactctttgg caaaacgacc agcatcgaag
    34681 tgcgggcgac gttcgcagtg ttcgtgaatc agacggccat aggcgaaggt cggacggttt
    34741 ttgtcatcca gtttcggcgg tttgccgtaa gtgatgatgt gcgcaacggt cgcgaggaag
    34801 ttgtgcgggt tcggcgggca gcccggaata ttgataacgg ttttgcctgg cagaacttct
    34861 tgcaagctga ctgcgccagt tgggttaact ccagctgcgg caacaccgcc ccacgcggag
    34921 caggaaccaa tagcgataat ggctgctgcg ccttccgccg ctttgcggat gtgatccaca
    34981 atcggctcac cggcaaccat gcaataaata ccgttatctt ttaatgggat ggaaccatcc
    35041 accactaaca catactgccc tttgtacttc tcgagagcgt tatgtttgtt ctcttcgacc
    35101 tgatgaccga aggcggcgga aagcacttcg tgatattcca gagagatagt ctccagcacg
    35161 aggttttcta ccgttggatg cgttgcacga agcagagatt ccgtacaacc ggtgcactcc
    35221 tgcgcgccaa tccagataac tggcggacgc tgcgggttag taaccgattc ggccatctct
    35281 gcagcggctt tgctacttaa ccccatggtg gcggctaatg ctgcacaaag cttcatgaaa
    35341 tcacgacggt taatgccgtg agaatggatg agggtgttat ctccagtcat ttatagttat
    35401 tccgttgcga ggacctggca tatattttgc ctcaatcgca aaatcaataa tgcgatcgat
    35461 gcgccattta ccacacattt attatggtta tcgatatcat gatactgcgg cggggcgcta
    35521 aaacgaaggg aagaagcatt agtgtaatta attaaagcaa gataatacgt atgtttgatc
    35581 aattttcgag gcgataaccg gccataaaaa agcgcaacgt agaacaggaa ttattctcac
    35641 gaaagaaatc cattaccatc tctttatcca gtccatagcg gcgcgtcaga atacccgctt
    35701 cccaggcgct aagctggcga tctccggtct tttcgtacat ccggtgtgag tagcctaata
    35761 caaaaccgcg tttataatcg gcacagaatt ttgtcacatt aaccgcactg tcggcatgcg
    35821 ttgcttttaa tcccgccatt aagcctttac caaaatggtt attcatcgtt cacctcattc
    35881 gttatatatc gccatatata gcgatatttt aggcaaagaa cgacttttgc gcacgctatt
    35941 ttttcatact tgtttaagac gtaatcccgt aagagataaa cctatcagga gagatattta
    36001 tcgaaccatt tcagtgtcct ttgccaggca agatcggcgg cagatttgtc ataacggggc
    36061 gtggaatcat tatggaatcc gtgattaacc cccggataga tataagcctc ataaacctta
    36121 ttattgtctt tcaacgccgc ctcgtaagga ggccagccct cgttgattcg ggtgtccagt
    36181 tccgcgaagt ggagtagtaa aggcgcttca atcttcgcca catcggcagt gggtgcctga
    36241 cgaccataaa acggcaccgc gcaggccagt tccggatacg cgacagccgc cgcgttcgat
    36301 acgccaccgc catagcaaaa tccggtaata cccactttac ctgtcgcttg cggatagcgt
    36361 tgcataaact caattgcggc aaagaaatca ttcatcagtt tggttggatc aacctgctgt
    36421 tgcagctcac gacctttatc atcatttccc ggataacctc caacggaact taatccgtca
    36481 ggtgccaggg cgatataccc cgccttcgcc actcgccgtg ccacatcttc gatatacgga
    36541 ttcagtccac gattctcatg caccaccccc acggctggcg ttttgccgct catttttgcg
    36601 ggtttcacca gataaccccg tacctcgccg tgaccatttg gcgaaggata cgtgatgtac
    36661 tcagcaacaa tctccgggtc ggtaaactct acctgagtcg ccagcgcata atttggcttg
    36721 agcaaatcaa acaacgctaa tgccgtcatc ccgcccaccg catacttcgc cgcaaggttg
    36781 aggaactcac gtttcgaaat tttcccgtga acgtaatagt cgtagtaatc caacaactct
    36841 tgtgggaaat ctttggcggt taaacgcggc atcgttgcat tcctcaactg gtgttttttt
    36901 aagcaaagca taagcgcgtg tttttgccca gtttttcgtc actctgtgag ccagactacg
    36961 ggatacgcgc tggcgaatcg ctaaactaga aacattgttt cgaaattgaa cggtggaaag
    37021 gagaggtcat ggtctggtta gcgaatcccg aacgttacgg gcagatgcag taccgctatt
    37081 gcggaaaaag tggtttacgc ctgcccgtgt tatcgctcgg tttatggcac aatttcggtc
    37141 acgttaacgc gctggaatca cagcgtgcaa tcctgcgtaa agcgtttgat ttaggcatta
    37201 cgcactttga tttagccaac aattacgggc cgcctccagg aagcgcagaa gagaactttg
    37261 gtcgcctgct gcgcgaggat tttgctgctt atcgcgatga actgattatc tctaccaagg
    37321 ctggctacga tatgtggccc ggcccgtacg gctctggcgg ttcacgtaaa tacctgctcg
    37381 ccagcctcga ccaaagccta aagcgtatgg ggcttgagta tgtcgatatc ttttactctc
    37441 atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc tgcgctggct catgcggtac
    37501 aaagcggtaa ggcgctgtat gtcgggatct cctcttactc gccagagcgg acgcaaaaaa
    37561 tggtcgagtt gctgcacgag tggaaaattc cgcttttaat tcatcaacct tcgtacaatt
    37621 tactgaaccg ctgggtggat aaaagcggcc tgctggatac cctgcaaaat aacggcgtgg
    37681 gctgtattgc ctttactcct ctggctcagg gattgctgac cggaaaatat ctcaacggca
    37741 ttccgcaaga ttcacggatg catcgtgaag ggaataaaat tcgtggtctg acgccgaaaa
    37801 tgcttaccga agccaacctc aacagcctgc gcttattgaa tgaaatggca cagcagcgtg
    37861 gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa agatgatcgc gtgacgtcgg
    37921 tattgattgg tgccagccgc gcggagcaac tagaggagaa cgtgcaggcg ctgaataatc
    37981 tgacatttag caccgaggag ctggcgcaga ttgatcagca tatcgccgat ggcgaactga
    38041 atctgtggca ggcgtcgtcc gataaatgac ctgttaataa cgggcgcgcg aaccgcgccc
    38101 gaatcagatc agtgattatg acgagtcagt cggtcaagat agcccatcac aaatgcagaa
    38161 agcacaaacg tcagatggat aatgacgtac cacatcagtt tgttatccgg gacatttttc
    38221 gcatccataa agacgcgcag taagtgaatg gaagaaattg ccacaatcga cgctgctact
    38281 ttgtttttca gcgacgttgc gtccattttc cccagccagt tcagcttctc tttgttctcg
    38341 gagatatcca gctgtgagac gaaattctca taaccggaaa acatcaccat caccagcaaa
    38401 ccgccaacca gcgtcatatc caccagcgac agcaacacga ggatcaaatc tgattccgcc
    38461 atcgagaaga tattcggcag tacgtgaata atctcctgga agaacttcag cgccagggca
    38521 actaacgcca gcgaaaggcc aaagtacacg ggggcaagca gccagcgaga agcatacatt
    38581 gcattttcaa gaaaacgttc catacattcc tgtcgtgtag ttaaaacggg caacagtata
    38641 tcgcaattga gtaaacagca cgcaacagca aatcaacata tacgttaaac ctttgtcgca
    38701 taattctgag gtttagggat cttcctggtt cccttatcaa cgcatcgaga gcgtctaccc
    38761 ttaaaggacg ttgaacctga agggagaaaa acgatgtctc atttaaaaga cccgaccacg
    38821 cagtattaca ctggtgaata tcccaaacag aaacaaccga cgccaggcat ccaggcgaag
    38881 atgacaccga taccggattg cggcgagaaa acctatgttg gtagcgctcg cctgaaagat
    38941 cgtaaagcac tggtgacagg gggcgattcc ggaataggtc gcgctgccgc catcgcttac
    39001 gcgcgagaag gggctgacgt ggcgatcagt tatcttcccg tggaagaaga agacgctcag
    39061 gatgtgaaaa agatcattga agaatgcgga cgcaaagccg ttctgctgcc aggcgattta
    39121 agcgatgaga aattcgcccg ttcgctggtt cacgaagcgc acaaggcgtt aggcgggctg
    39181 gatattatgg cgctggtcgc cgggaaacag gttgccattc cggatattgc agacctcacc
    39241 agcgaacagt ttcaaaagac ctttgccatt aacgttttcg cgctgttctg gctaacccag
    39301 gaagcgatcc ccctgctacc gaaaggtgca agtatcatca ccacttcgtc aatccaggca
    39361 taccagccaa gtccgcattt actggactat gcggctacga aggcggcgat tctgaactac
    39421 agccgtggcc tggcaaaaca ggtcgcggag aaaggtattc gggtgaatat tgtcgcgcca
    39481 ggccctatct ggacggcact gcaaatttcc ggcggacaaa cgcaggataa gatcccgcag
    39541 tttggtcaga aaacgccgat gaagcgtgcg gggcaaccgg cggaactggc ccctgtatat
    39601 gtttatctgg caagtcagga gtcgagctac gtcaccgcag aagtgcacgg cgtgtgcggc
    39661 ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag caaccgggca tcattgtgaa
    39721 ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct acaaacgcat gaagattcaa
    39781 catgttgcaa tagattttgt aggcctgata agcgtagcgc atcaggcatt gagcacctaa
    39841 tgccggatgc gtcgtgaacg ccttatccgg cctacaaacg cacgaagatt caacatgttg
    39901 caatagattt tgtaggcctg atgagcgtag cgtatcaggc aattctactt tacttcgctt
    39961 tggcggtttc ttcgccgacc agacctatct tcaggtaacc cgcctgatgc agcgtatcca
    40021 ttaccttcat caacgtctcg taatcgacgg ttttgtccgc tcggaagaag atggtggtgt
    40081 ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat tgtttcatcg gtgaccggat
    40141 cgttaccgat aaacatcgag ttgtctgcct tcaccgacag ataaaccggt ttttccgggc
    40201 gcggctgcgg cgtgctggta gaagcaggca agttcacctt cacatctacc gtcgctaacg
    40261 gtgccgccac cataaagata atcagcagaa ccaacatcac gtcgataaac ggcgtcacgt
    40321 tgatatcatg catttcgccg ttatcgtcga ggttttcgtt aagatgcatt gccatcggac
    40381 attatcctgc gcgtaatttt tgtgcgacac gaaccggatg cgcagcggcg ctggcttcca
    40441 gatccaggtc acggctttgc agcaacaata cctgcgctgc aacatcaccc agcatcgctt
    40501 taaagccgcc aatctggcgt gcaaatacgt tatagataac gaccgcagga atcgctgcca
    40561 cgaggccgat tgccgttgct aacagagctt ctgcgatacc cggcgcaacg actgccaggt
    40621 tagtggtctg cgtttgcgcg ataccaataa agctgttcat gatgccccag accgtaccaa
    40681 acagaccaac gaacggagaa atcgcgccga tggttgccag atagccgtta ccgcggccca
    40741 tttgacgacc cactgcggcg acccgacgct ccagacggaa gctggtacgt tctttaatac
    40801 cttcgttatc gtcgctgcct tctgacagtt ccagctcatt ctgcgcttca ttgagcaaat
    40861 gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc gttggcctgg tttaaggaac
    40921 gtgcttcagc cagcagttgc tgctcgcgct taaggcgacg cttctgattg aagaactcta
    40981 cgctcttact gaagaagatt gcccaggtga ctacggaggc caaaataagc ccaatcatca
    41041 cgcacttaac gacaatatcg gcgtgctgat acatacccca gacggaaagg tccgtctgca
    41101 ttaaattatt acccactgtg tatctccagg acgcaagtca caaaatctgc gcataataat
    41161 atcaaaacga cgtcgaattg atagtcgttc tcattactat ttgcatgctg ccgtaccttt
    41221 gctttctttt ccttgcgttt acgcagtaaa aaagtcacca gcacgccatt tgcgaaaatt
    41281 ttctgcttta tgccaattct tcaggatgcg cccgcgaata ttcatgctag tttagacatc
    41341 cagacgtata aaaacaggaa tcccgacatg gcggacaaaa agcttgatac tcaactggtg
    41401 aatgcaggac gcagcaaaaa atacactctt ggcgcggtaa atagcgtgat tcagcgcgct
    41461 tcttcgctgg tatttgagag tatggaagcc aaaaagcacg cgacgcgcaa tcgcgccaat
    41521 ggcgagttgt tctatggacg gcgcggaacg ttaacccatt tttccttaca acaagcgatg
    41581 tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct gcggggcggc agcggttgct
    41641 aattcgattc ttgcttttgt cgaacagggc gatcatgtgt tgatgaccaa caccgcctat
    41701 gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac tgggcgtaac gacatcgtgg
    41761 tttgatccgc tgattggtgc cgatatcgtt aagcatctgc agccaaacac taaaatcgtg
    41821 tttctggaat cgccgggttc catcactatg gaagtccacg acgttccggc gattgttgcc
    41881 gccgtgcgca gtgtggtgcc ggatgccatc attatgatcg acaacacctg ggcagccggt
    41941 gtgctgttta aggcgctgga ttttggcatc gatgtttcta ttcaggccgc caccaaatat
    42001 ctggttgggc attcagatgc gatgattggc actgccgtgt gcaatgcccg ttgctgggag
    42061 cagctacggg aaaatgccta tctgatgggc cagatggtcg atgccgatac cgcctatata
    42121 accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc aacatcatga aagcagtctg
    42181 aaagtggccg aatggctggc agaacatccg caagttgtgc gagttaacca ccctgccctg
    42241 cctggcagta aaggacacga attctggaaa cgagacttta caggcagcag cgggctattt
    42301 tcctttgtgc ttaagaaaaa actcaatgat gaagagctgg cgaactatct ggataacttc
    42361 agtttattca gcatggccta ctcgtggggc gggtatgaat cgttgatcct ggcaaatcaa
    42421 ccagaacata tcgccgccat tcgcccacaa ggcgagatcg attttagcgg gaccttgatt
    42481 cgcctgcata ttggtctgga agatgtcgac gatctgattg ccgatctgga cgccggtttt
    42541 gcacggattg tataacattg ccacttttgg acaattttgc agacatttta ttgtgaaaag
    42601 tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga ttcaggagta caataggcag
    42661 ataaaggctt aaacgctgtt ccacaggaaa gtccatggct gttattcaag atatcatcgc
    42721 tgcgctctgg caacacgact ttgccgcgct ggcggatcct catattgtta gcgttgttta
    42781 ctttgtcatg tttgccacgc tgtttttaga aaacggcctg ctgcccgcct catttttgcc
    42841 aggcgacagc ttgttgatac tggcaggcgc attgattgcc caaggggtta tggattttct
    42901 gcctacgatt gcgattctga ccgccgcagc aagtctgggc tgctggctaa gttatattca
    42961 ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg ctggcacagc ttcctgctaa
    43021 atatcaccag cgcgccacct gcatgtttga ccgccacggt ctgctggcgc tgctggctgg
    43081 acgttttctt gcatttgtcc gtacgctgct gccaaccatg gcgggaattt ccggtctgcc
    43141 aaaccgccgc ttccagtttt tcaactggtt aagtggattg ctgtgggtca gcgtggtaac
    43201 cagttttggc tatgccttaa gtatgattcc gttcgttaaa cgccatgaag atcaggtaat
    43261 gacgttcctg atgatcctgc caattgcctt gttaaccgct ggcttgttag gcacgctgtt
    43321 tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt tccccgttcc cggctgctgt
    43381 accgggaacg tatttaattc ccctgcatcg cccgcattct tgccgcatct tcccccggcg
    43441 tcacaccgaa gtaacgttta aactcacggc taaattgcga tgcgctttca tagccgacgc
    43501 gcatcgctgc tgcgctggcc ttcatgccgt catggatgat catcatccgc gccttatgca
    43561 gacggtaatt cttcaaatac tgcaacggcg aggtgctggt gacagactta aaattatggt
    43621 ggaacgccga tacgctcatg ttggcttctg ccgccagttg ctcgacgctc aggttttcgg
    43681 tgtatttatt ctcaattcgt ttcagcacgc ggctaatcag actgaagtga gtctggcgac
    43741 tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag cacgtagtac agaatttcgc
    43801 ggatgatctg tttgccgaga atacgcgcat ccagtggtcg ctccatcacg tcgagtaacc
    43861 gctccgccgc gaataaaatc tcttctgata acgtggcgga gttaatcccg ctggctgcca
    43921 tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa cagttcctgt aactgcaaaa
    43981 tatcgacatt gagacgcaac cctgccagcg gcacctctga cgtggcatag gtttcgcact
    44041 caaacggcaa cggcaccgtc agcagcaggt attcattggc atcataacga
//
LOCUS       DAJTYG010000022        42812 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTYG010000022 DAJTYG010000000
VERSION     DAJTYG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.022"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..520
                     /locus_tag="OO568_001322"
                     /pseudo
     CDS             1..520
                     /locus_tag="OO568_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
     gene            524..1336
                     /locus_tag="OO568_001323"
     CDS             524..1336
                     /locus_tag="OO568_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640747.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(1348..2952)
                     /locus_tag="OO568_001324"
     CDS             complement(1348..2952)
                     /locus_tag="OO568_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR6640748.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPCEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(3078..3383)
                     /locus_tag="OO568_001325"
     CDS             complement(3078..3383)
                     /locus_tag="OO568_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6640749.1"
                     /translation="MATLLQFHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(3491..3567)
                     /locus_tag="OO568_001326"
     tRNA            complement(3491..3567)
                     /locus_tag="OO568_001326"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3531..3533),aa:Val,seq:gac)
     gene            complement(3572..3648)
                     /locus_tag="OO568_001327"
     tRNA            complement(3572..3648)
                     /locus_tag="OO568_001327"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3612..3614),aa:Val,seq:gac)
     gene            3956..5212
                     /locus_tag="OO568_001328"
     CDS             3956..5212
                     /locus_tag="OO568_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6640750.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYAYGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYCLGT
                     DGLQLYSSGKTQNLSVNVGDRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(5253..6626)
                     /gene="mdtK"
                     /locus_tag="OO568_001329"
     CDS             complement(5253..6626)
                     /gene="mdtK"
                     /locus_tag="OO568_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6640751.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFISLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            6841..7482
                     /gene="ribE"
                     /locus_tag="OO568_001330"
     CDS             6841..7482
                     /gene="ribE"
                     /locus_tag="OO568_001330"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6640752.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(7522..8670)
                     /gene="cfa"
                     /locus_tag="OO568_001331"
     CDS             complement(7522..8670)
                     /gene="cfa"
                     /locus_tag="OO568_001331"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6640753.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8961..10172)
                     /locus_tag="OO568_001332"
     CDS             complement(8961..10172)
                     /locus_tag="OO568_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6640754.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRTALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            10285..11217
                     /locus_tag="OO568_001333"
     CDS             10285..11217
                     /locus_tag="OO568_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6640755.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(11214..12239)
                     /gene="purR"
                     /locus_tag="OO568_001334"
     CDS             complement(11214..12239)
                     /gene="purR"
                     /locus_tag="OO568_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6640756.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            12538..12627
                     /gene="ynhF"
                     /locus_tag="OO568_001335"
     CDS             12538..12627
                     /gene="ynhF"
                     /locus_tag="OO568_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6640757.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            12793..13944
                     /locus_tag="OO568_001336"
                     /pseudo
     CDS             12793..13944
                     /locus_tag="OO568_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(14090..14671)
                     /gene="sodB"
                     /locus_tag="OO568_001337"
     CDS             complement(14090..14671)
                     /gene="sodB"
                     /locus_tag="OO568_001337"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6640758.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(14799..15626)
                     /gene="mepH"
                     /locus_tag="OO568_001338"
     CDS             complement(14799..15626)
                     /gene="mepH"
                     /locus_tag="OO568_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6640759.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATAHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            15961..16308
                     /gene="grxD"
                     /locus_tag="OO568_001339"
     CDS             15961..16308
                     /gene="grxD"
                     /locus_tag="OO568_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6640760.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(16428..16847)
                     /gene="tnpA"
                     /locus_tag="OO568_001340"
                     /pseudo
     CDS             complement(16428..16847)
                     /gene="tnpA"
                     /locus_tag="OO568_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            16855..18063
                     /locus_tag="OO568_001341"
     CDS             16855..18063
                     /locus_tag="OO568_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6640761.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNAAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            complement(18107..22724)
                     /locus_tag="OO568_001342"
                     /pseudo
     CDS             complement(18107..22724)
                     /locus_tag="OO568_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(22817..23464)
                     /gene="rnt"
                     /locus_tag="OO568_001343"
     CDS             complement(22817..23464)
                     /gene="rnt"
                     /locus_tag="OO568_001343"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6640762.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(23567..23974)
                     /gene="gloA"
                     /locus_tag="OO568_001344"
     CDS             complement(23567..23974)
                     /gene="gloA"
                     /locus_tag="OO568_001344"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6640763.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(24055..25152)
                     /gene="nemA"
                     /locus_tag="OO568_001345"
     CDS             complement(24055..25152)
                     /gene="nemA"
                     /locus_tag="OO568_001345"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6640764.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGRAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(25189..25788)
                     /gene="nemR"
                     /locus_tag="OO568_001346"
     CDS             complement(25189..25788)
                     /gene="nemR"
                     /locus_tag="OO568_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6640765.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            25891..26130
                     /locus_tag="OO568_001347"
     CDS             25891..26130
                     /locus_tag="OO568_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6640766.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            26179..27075
                     /locus_tag="OO568_001348"
     CDS             26179..27075
                     /locus_tag="OO568_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6640767.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGMLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            27156..27677
                     /gene="sodC"
                     /locus_tag="OO568_001349"
     CDS             27156..27677
                     /gene="sodC"
                     /locus_tag="OO568_001349"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6640768.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(27678..29660)
                     /locus_tag="OO568_001350"
                     /pseudo
     CDS             complement(27678..29660)
                     /locus_tag="OO568_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(29653..30444)
                     /locus_tag="OO568_001351"
                     /pseudo
     CDS             complement(29653..30444)
                     /locus_tag="OO568_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(30447..30683)
                     /locus_tag="OO568_001352"
     CDS             complement(30447..30683)
                     /locus_tag="OO568_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6640769.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            30884..31318
                     /gene="slyA"
                     /locus_tag="OO568_001353"
     CDS             30884..31318
                     /gene="slyA"
                     /locus_tag="OO568_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6640770.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(31365..31832)
                     /gene="slyB"
                     /locus_tag="OO568_001354"
     CDS             complement(31365..31832)
                     /gene="slyB"
                     /locus_tag="OO568_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6640771.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            32106..33215
                     /gene="anmK"
                     /locus_tag="OO568_001355"
     CDS             32106..33215
                     /gene="anmK"
                     /locus_tag="OO568_001355"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6640772.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            33313..33642
                     /gene="mliC"
                     /locus_tag="OO568_001356"
     CDS             33313..33642
                     /gene="mliC"
                     /locus_tag="OO568_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6640773.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGFSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            33701..34357
                     /gene="pdxH"
                     /locus_tag="OO568_001357"
     CDS             33701..34357
                     /gene="pdxH"
                     /locus_tag="OO568_001357"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6640774.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            34486..35760
                     /gene="tyrS"
                     /locus_tag="OO568_001358"
     CDS             34486..35760
                     /gene="tyrS"
                     /locus_tag="OO568_001358"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6640775.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            35819..36688
                     /gene="pdxY"
                     /locus_tag="OO568_001359"
     CDS             35819..36688
                     /gene="pdxY"
                     /locus_tag="OO568_001359"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6640776.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQV
                     KAANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAV
                     NNVEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGM
                     RQPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDR
                     IAKPEHYFSATKL"
     gene            complement(36732..37337)
                     /gene="gstA"
                     /locus_tag="OO568_001360"
     CDS             complement(36732..37337)
                     /gene="gstA"
                     /locus_tag="OO568_001360"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6640777.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(37443..38945)
                     /gene="dtpA"
                     /locus_tag="OO568_001361"
     CDS             complement(37443..38945)
                     /gene="dtpA"
                     /locus_tag="OO568_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6640778.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(39556..40191)
                     /gene="nth"
                     /locus_tag="OO568_001362"
     CDS             complement(39556..40191)
                     /gene="nth"
                     /locus_tag="OO568_001362"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6640779.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(40191..40886)
                     /gene="rsxE"
                     /locus_tag="OO568_001363"
     CDS             complement(40191..40886)
                     /gene="rsxE"
                     /locus_tag="OO568_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6640780.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(40890..41510)
                     /gene="rsxG"
                     /locus_tag="OO568_001364"
     CDS             complement(40890..41510)
                     /gene="rsxG"
                     /locus_tag="OO568_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6640781.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFGQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(41514..42572)
                     /gene="rsxD"
                     /locus_tag="OO568_001365"
     CDS             complement(41514..42572)
                     /gene="rsxD"
                     /locus_tag="OO568_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6640782.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(42573..>42812)
                     /locus_tag="OO568_001366"
     CDS             complement(42573..>42812)
                     /locus_tag="OO568_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6640783.1"
                     /translation="AKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQAN
                     AEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
BASE COUNT        10773 a        10705 c        11123 g        10211 t
ORIGIN      
        1 aagtgggctg ataaatcact ttgcgatggt gggcgcaact gcggttaaaa gtctggttgc
       61 ggtgcatgaa gtttgctgat ttttgttact ggcatgctgg ctcggctttg tgctgatcaa
      121 tgccgttggg gataatggtc accactatcg cattcgtcgt caggggtggc tggaacgagc
      181 ggcaaaacaa acgcgatttt atttgtttgg cattatgcag ggggaagaac atcctttccc
      241 ggcaacaaca cagtctaaat ttaatccctt acagcaggtc gcctatgttg gtgtcatgta
      301 tggattgctg ccgttgttac tattgacggg gctgttgtgt ctctatccgc aagccgtggg
      361 agatgtgttt cctggcgtaa gatactggtt attgcaggcg cattttgctc tggcatttat
      421 aagcctcttt tttatcttcg gtcatcttta tctttgcacc acggggcgta cgccacacga
      481 aacctttaaa agcatggtcg atggctatca ccggcactaa cgaatgatca ttacacgagc
      541 agacctgcga gaatggcgta tcggagcggt gatgtatcgc tggttcctgc gccatttccc
      601 acgcggcggt agttatgccg atattcgtca tgcactgatt gaggaaggat atacggactg
      661 ggcggagagt ctggttgagt atgcgtggaa aaaatggctg gcggatgaaa acttcgcgca
      721 tcaggaagtt tcctctatgc aaaaactggc cacagacccc ggagagatac cattttgcag
      781 tcagtttgcc cgcagcgacg atcatgctcg catcggctgt tgtgaggata acgcccgaat
      841 cgcaaccgct gggtatgccg cgcaaattgc cagtatgggt tatagcgtgc ggattggaag
      901 tgtcggcttt aacagtcaca ttggcagctc cggtgaacgc gcacgggttg ctgtaaccgg
      961 taattcttcg cggataagca gcgctggaga tagtagtcgg attgcgaata ccggaatgcg
     1021 agtacgcgtt tgtacattgg gtgaacggtg tcatgtcgcc agtaatggtg acctggtgca
     1081 aatcgccagc tttggcgcaa acgccagaat tgccaacagc ggcgacaatg tacacatcat
     1141 tgccagcggt gaaaattcaa cggtagtgag cacgggcgtg gtcgactcaa ttattctcgg
     1201 gctaggggga agcgccgcac tggcatatca tgacggcgaa agagttcgct tcgccgtcgc
     1261 cattgaaggt gaaaacaata ttcgaaccgg agtccgctat cgtcttaatg aacagcatca
     1321 gtttgttgag tgctgatagc tttaccttta atcaaacgaa agacgccgac gagcacggga
     1381 tgcaggcttt acgaccgccc gagccatcgc ctcaccaatt tctgcacatg ccgtaagtcc
     1441 ctgaacgaaa ggctggtcgt gcatcagcca gggcaacgcg ccgaacgcta cgcgtccacg
     1501 aatatcttcg ggttgctgta acgtataatc ttcgccaaca tcagggattt catcccctgt
     1561 tttctgtaat tgctcgcgta gcccagggaa aggaatatct ttcactttaa gcggacgctg
     1621 tccgcgggca tcaataaaaa cgtcaaacga gtagctgttg tcttccgttt tcaggacggt
     1681 gcgcgactca ttaatttcca tttcgtagtc ttcaccgagg gcgagaatat gaatgatccc
     1741 cgcttcacgt aaggccagta gtcgacgaat agactctgaa gggattgccg cataattatc
     1801 gatgaaaacc ctggcaaggc ctttactgaa ccgtttatgg tcgtgttcat tcagatgagg
     1861 aacaatttcc tgtacggctt catgcaggcg cagaatgaca taacgccagg gaacagtatg
     1921 tttttctcgt ttattgcgtt cgacttcctg gagatttttt tctgcccagt caaatgggtc
     1981 gcgttgtttg cgctcggcaa accaggcttg agcaaaggaa tcgacattca ggctctctaa
     2041 ggctatgcgt tggctccagt ctggatcagc aaacttgatt tcctctacta tcaatctaaa
     2101 tacccgatcc aaaaggccct cttcaccttt ttgaatctca gcatttaatg cctgatcggt
     2161 gacgatgtgt aagggctcgc agggaatagg gcaatagaaa tcggcttcgg gtaaaatacc
     2221 cgtgcgcgac atcaacgtaa tatttagctt ttcacttgcg ttatcgcggt gaaaaacgac
     2281 gtgttgttta tcatcttcaa tgaacgaacc atgctgaata gccactgcca ttgccgcatc
     2341 cagtccgctc aaggatgttc ccataatacc cacgttacac gcatcgacct ttgcttccat
     2401 caggcctgac cacgggctgg gaaaatacgt tctggttgct tcttcttcat caggccagac
     2461 gtgacccgtg gcaatcaccg ctaaatcaaa cgtctcgctg ggtaaatcct gatttgtagc
     2521 gagcatgacg ccagcatttg taatttgcag atcggtaacc tggcatgatt cataaacagc
     2581 cactgcaaat ttttgctgtc gtgcctggtc tactaatcgt aaaaattgat cgcggaaata
     2641 ttcgcccagc agaattcgcg gtaaaaactg acgatcgtgc aaggtttctt ttttaacgcc
     2701 ataacgctgg agatggctgg cttcttgctt ttgtagccat tcgagatacg tacaattaat
     2761 cggcggtatt tcaatactgg caatatttgc cagcatcatt tttgagtttt cctcatcact
     2821 gtatggcatc ccgacaccgg cctcgtcagc ctgctcgaag atagaaatag aaagtggagt
     2881 ttgttgctgt agaagcgaga ataaggtgta gatccccgta ggcccggcac ccacaatagc
     2941 aatttttttc attgttactt acctgtacgc attctttcga atttagtgtt atcagataag
     3001 tttggttgag atttttagaa tttgagaaat ttcagacagg gatgaaaagg gcgctaaccg
     3061 cccttgatta aattctgtca ggcgagtttt gcctgattga tttgactaag tggctcattg
     3121 acatcgaaaa ctttggcgac aacttcctca acgccgagat ttttcagtcg ggcagtatgt
     3181 ttttccagat aggcaagggc gcttttttca tcagtgaaaa ggtagatccc accggcttcg
     3241 tggttctttt cactttctgt ccataccttc cacagaaaac caggttcctg attaatcgac
     3301 tcagcaagtg gtttaagctg ctcagccatt gcgtcgccaa atgggccatt aaaagcaaaa
     3361 tgaaattgta acaaagttgc catagcgatt ctccttctga taacgcagac gaagacagtg
     3421 taaatctttt aacgtattgg atacagagaa gaaacagagg gaaaaaggaa aagaccagat
     3481 taagaaaatc tggtgcgtcc gagtggactc gaaccaccga cccccaccat gtcaaggtgg
     3541 tgctctaacc aactgagcta cggacgcagg atggtgcgtt caattggact cgaaccaacg
     3601 acccccacca tgtcaaggtg gtgctctaac caactgagct atgaacgcaa cgttgtaggt
     3661 gacaacgggg acgaatatta gcggcagagt ggaaaggtgg caagaggcaa aacgtaattt
     3721 tctgcgctat ttcgaccgtt tgcagagctt ttaagcaaat tggctatatt ttgttgattt
     3781 gcaagggcga tttttattca ggatcgcatt tacatctgat acaacccaat aattcgtgta
     3841 tcagagttta aacagagttg tttgccacca tctcaaatct gtcgtggata aatcctccgt
     3901 agcctttaca cttaggtagc aataaaggca cacagatgca gcaaaggaat aaatcatggg
     3961 atctgatgcg aaaaacttga tgagcgacgg gaatgtgcaa attgttaaga ccggcgaggt
     4021 cattggcgcg acgcaactta ctgaaggcga gttgattgtt gaagctggcg gaagagccga
     4081 aaataccgtg gtcacggggg ctggctggtt gaaagtggca accggtggga tcgccaaatg
     4141 cacacagtac ggtaacaatg gcacgctatc ggtcagcgac ggtgccattg ccacagatat
     4201 tgttcagtcc gagggaggcg caattagtct ctctacgctc gctacggtta atggccgcca
     4261 tcccgaaggt gaattcagcg ttgatcaggg ttatgcctac ggtttgttgc tggaaaatgg
     4321 cggtaacctg cgtgtactgg aaggacatcg cgcggaaaaa atcattctcg atcaagaggg
     4381 cggcctgttg gttaatggga caacctcagc ggtcgtggta gatgaaggtg gtgaattgtt
     4441 ggtgtatcca agtggagaag ccagcaattg tgagattaat cagggcggcg tttttatgct
     4501 ggccgggaaa gccagtgata cgttgcttgc tggtggcacc atgaataatc tcggtggtga
     4561 agactctgac actattgttg agaatggatc catttattgt ctggggacgg atggccttca
     4621 gctctacagt tccggtaaga cgcaaaacct gtcggtgaat gtgggggatc gggctgaagt
     4681 gcatgccggt acgctggaaa atgcggtaat acaaggtgga acagtgatcc tgttgtcacc
     4741 caccagcgcg gacgaaaatt ttgtcgtaga ggaagatcgc gcaccggttg aactgaccgg
     4801 gagtgttgca ttactggacg gcgcttcaat gattattggt tatggcgcag agctgcaaca
     4861 atcaacgatt actgtacagc agggcggtgt gttgattctc gacggcagta cggtaaaagg
     4921 tgacagcgtc actttcattg ttggtaacat caatctgaat ggcggaaaac tgtggctgat
     4981 cactggtgcg gcaacgcatg tgcaactgaa agtgaaacgc ctgcgcggag agggagcgat
     5041 ttgcctgcaa accagtgcga aagaaatttc acctgacttc atcaatgtga aaggggaagt
     5101 taacggggat atacgcgttg agataacaga tgccagtcgg caaactctgt gtaacgctct
     5161 gaaattacag ccagacgaag acgggattgg cgcaacgctc cagcctgcgt aaaaacacag
     5221 gcgcaacccg tgaaggttgc gccttgtctt tattagcggg acgctcgttg cagaatgatg
     5281 gctgacggca tacgttgcag gaaccgcata cgcagcatca tcataatggc tgccgatgtc
     5341 aggccgataa taaagcctat ccagaagcct gctggcccca taggttcaac gaccagatcg
     5401 gtcagtgcca gaatatagcc gcttggcaag cccagcaccc agtaagctgt aaaggtgata
     5461 tagaaaatgg aacgcgtatc tttataacca cgcaaaatcc cactgccaat cacctggatt
     5521 gagtcagaaa tctgatatac cgccgccagc aacatcaaat gcgcagccag cgttacaacc
     5581 tcgggattgt cgttgtacaa cagggcgatt tgctcccgca gtgaaaccgt gaaaatggcc
     5641 gtcagggttg ccatacagac acccaccata agcccggtcc gcgcagcggt ttgcgcatcc
     5701 agcgttgagc cctgacccag acgataacct acgcggatag ttaccgctgc cgccagcgac
     5761 attggaagca caaacatcag tgaactaaag ttcagggcaa tctggtgtcc tgcgacatca
     5821 acaataccga gcggagacac taacagagcc acgacggcaa acagtgtcac ttcaaagaac
     5881 agtgccagcg caatcggcaa accgagttga atcagtcgtt tcataaccgc aggatcgggc
     5941 ttagcggtgc ctttttcgtt acgaatatcg cgcatggagc gggcgcgttt aatgtaagaa
     6001 accatggcaa ggaacatgac ccaatacacc gccgcagtag ccacgccaca accaacgcca
     6061 ccgagctcag gcataccgaa atgaccataa ataaagatat agttcaccgg gatgttcacc
     6121 agcaggctga taaagcccat taccataccc ggcttggttt ttgccagacc ttcacattgg
     6181 ttacgggcaa cctggaagaa cagatatccc ggcgcgcccc acaacaacgc acgcagataa
     6241 cccacggctt tgtccgccag agccggatcg atgttttcca tggagcggat aatgtaacct
     6301 gcattccaca gcaccagcat aatgagaacg gaaacaaaac ctgccagcca gaaaccttgt
     6361 cgcacctgat gcgcaatgcg ctcacgtcga ccggaaccat ttaattgcgc gataaccggc
     6421 gttaatgcca gcagcagtcc gtgaccaaag aggatcgccg gaagccagat agaagtaccg
     6481 atagcgaccg ccgccatgtc ggtggcacta tagccgcccg ccatcacggt atcgacaaaa
     6541 cccatcgcag tttgggcgat ttgcgcgaga atcaccggga ttgctaatgc taataacaga
     6601 cgcgcttcac tgatatactt ctgcacgtga acacctttta tttgtagtta tatgagagac
     6661 taaaaagccg cctgaatggg cagcaaaaag aagaagcagg ggaaattcag tctattgtag
     6721 cgaggattta ttaattctcc agtgaaaaaa tagccattct taccccttca ctggtaaggg
     6781 cgttttccac ctgctattgt gctgaacaga atgtcttaac tgatttcagg agttgtaagt
     6841 atgtttacgg ggattgtaca gggcaccgca aaactggtgt cgattgacga gaaaccaaat
     6901 tttcgtacgc atgtggtgga gttacccgac cacatgctgg acggcctgga aaccggtgct
     6961 tccgtggcgc ataacggttg ctgcctgacc gtgacggaaa ttaacggcaa ccatgtcagt
     7021 tttgacctga tgaaagaaac gttacgcatt accaatcttg gcgatttaaa agtgggggat
     7081 tgggtaaacg ttgagcgtgc ggcgaaattc agtgatgaaa ttggcggaca cttaatgtca
     7141 ggtcatatta tgaccaccgc tgaagtggcg aaaatattaa cctcggaaaa taatcgccag
     7201 atctggttta aagtccagga tagtcagttg atgaaatata tcctgtacaa aggatttatt
     7261 ggcatcgacg gtattagcct gaccgtcggc gaagtcacgc caacgcgttt ttgcgtccat
     7321 ttaattccgg aaacactgga acgcacgact cttgggaaga aaaaacttgg cgcacgcgtc
     7381 aacattgaaa tcgatccaca aactcaggca gtggtagata cggtagaacg tgtgctggcg
     7441 gcacgagaaa atgccatgaa tcaaccaggt acagaagcct gatctgaact cgcccccgaa
     7501 gagggggcga tagaatagcc tttagcgagc gactcgaagg ccgttttcaa caccgcgtga
     7561 gaacaccacc tgccagagct gaatatcacg ggcgcggaaa gcacctgcgc aggcattcag
     7621 ataataggta aacatccgtt taaagcgttc actgtagtta tccgcaattt ctggccatgc
     7681 ggcgaggaat cgttcatacc acgccatcaa cgtagtatcg taatcagcac cgaagttatg
     7741 ccagtcttcc atcacaaagt ggggttcgct ggattgagca atctggcgta cagagggcag
     7801 gcaaccgttc ggaaaaatat atttattaat ccagggatca acattcagat cggttttttt
     7861 cgaaccgata gtatggagca ggaatatacc ttccggtttc aaattacgat ccaccaccgc
     7921 aaaataggta tcgtaatttt tcggtccgac gtgctcgaac atccccacag aaacaatacg
     7981 atcaaactgg tcgttcaggt cacgataatc ttgcagcaaa atggtgacat ccaggccttt
     8041 acagcgttcc tgagccattt tttgctgttc ggcagaaatg gtgacgccga ccacgcttac
     8101 gtcatagtta gatgccatgt agtgcgccag accgccccag ccgcagccaa tatccagtac
     8161 gcgcatccct ggttttaact gcaatttttc acaaatcatt ttgagcttcg cctgctgggc
     8221 agattccaga ttatcggcat ctttccagta agcgcaggaa tattgcatga agggatcaag
     8281 catgcggctg aacaagtcat tacccaaatc gtaatgctcc ttgccgacta tccaggcacg
     8341 ttttttactc tgcagattga agagacgagc gccggcaata cgcagcgtgt ctttgaaatg
     8401 atgggggagt tggttctcga gacctgcgcg taagactttg ctaaaaaaca tatccagtcg
     8461 gtcacattcc caccagccat ccatataact ttcgcctaac cccaaagagc cttcctgcag
     8521 aacgcgttta aaaaaatcgg ggtttttcac acgaatatcc gccggggcag aaccgttaat
     8581 ggctataccg gcacggctaa gtaattcgtt ggcgatacgg taccagttgt catccggtac
     8641 actgacttct tctatacacg atgaactcat agtttctcca tcactggtcg gtgatcagaa
     8701 ccttaaaaca gcgtagacgc tttttgggct ttgtgagaaa tctcgcagaa aaaaccgcga
     8761 gtctataatc cgacgtagaa cagagggaaa ggaggaaccc ttgccgaaat gaccatccat
     8821 agtgagtcgg gaacgatcct gtccccgtaa aaacataata tttatcgtag ctttaacaac
     8881 tcgaaattag tataggccgc taatttcaat catacaacaa aattttgtta gcagcctaag
     8941 tataagtata tcgatataga tcagtgtgat tcgctatgag cgacttcggc attgccatga
     9001 ttcgggcagt caacttcttc acaacgttgc atcatgtaac ccagcgcgac cagcactact
     9061 gttgataaca tcacgctggt ggtggtgagc aatggcgtgc tgatactaat aagccaggaa
     9121 actaccagac ttgcgaggaa gcacagaccc agttgcagag tgttctgcaa cgctgcggcg
     9181 cgaccggttg catgtgggaa gggacgcagc gcctgggcga caacaatagg gtagatcgcg
     9241 ccattggcaa tcgccatcac acagaatggg atcaggattt cgaccagcga cacatggcta
     9301 atgaagcccg cagcccaggt cgcaatgacg ctgacagcaa acagcaccag caaccacggt
     9361 aataactgct tgccttgcca tttctgcagt gcggtgcgac agccataacc accaatcaga
     9421 aacgcgatag tttgcgggac ataacttaaa ccaataactg ccgggctgta gcccatttca
     9481 ctaaggatga acggtgaacc ggtcagccat gcaaaaaaac tggctgaaca ggctgcgtat
     9541 atcagcacgt tgccgcgata ggttttagaa cgtagcaggt cggtaaaggt cagaccatcc
     9601 tgactattgt tacgggcctt cgtcgtgggt ttgagccaga aaataggcag aatcagcacc
     9661 acggtaatgg caaacagggt ggcgaaaatc gcctgccagg aaaaatggac cagcagccag
     9721 cttcctaaca ggggagccag tgccggagac agacccacca gcggcatgat ggtcgcaaaa
     9781 atacggttga ctttctgtga aggataataa tctgtcacta atgcttgcca gataaccgcc
     9841 gcggcgcaga cccccacagc ctgtacaaaa cgcaatacca gcagcgtggc ggcgttttct
     9901 acccacagca tccccagact acctaacgca aaaattgtca ggccgattaa taacaccggt
     9961 ttacgaccat aacggtcgga gagtggcccc cacagaagct gggctgcggc aaaaccggca
    10021 aggaacagac taaggctggc actgacagca gacgcaggcg tttgcaggtc ggcctgtatg
    10081 gcggcgaaag caggcagata catatcggtt gccagaaaac cgagtacgct caaacccgcc
    10141 agccagacta aaaatctttt cccaggttgc atgtatattc tctaaatctc tcaattaaac
    10201 accgccgcaa agtgtaggga gtgaaattag tgttgtgaaa cggtaatatt tgatgactgg
    10261 tttcaaaaat tttgcaggct gactatgtgg tcagaatatt cactcgaagt tgttgatgcg
    10321 gtagcgcgta atggtagttt tagcgctgcg gcacaggagc tgcatcgcgt tccttctgcg
    10381 gtcagctata ccgtgcgtca gctggaagag tggctggcgg taccgctctt tgaacgacgg
    10441 caccgtgatg tggagctgac cgctgctgga gcgtggtttc tcaaagaagg gcgctctgtt
    10501 gtcaaaaaaa tgcagatcac ccgccagcaa tgtcagcaga tagcgaacgg ctggcgcggt
    10561 cagttagcta tcgcagtgga taatattgtc aggccagaac gtacacggca gatgatcgtt
    10621 gatttttatc gccattttga tgatgttgaa cttcttgtct ttcaggaagt gttcaacggt
    10681 gtctgggatg cgctttccga cgggcgcgtg gaactggcaa ttggcgcaac acgcgcgatt
    10741 ccggtaggcg gtcgttatgc cttccgggat atggggatgc taagctggag ttgcgttgtt
    10801 gccagtcacc acccgctggc gttgatggat ggcccgttca gcgatgatac gttgcgcaac
    10861 tggccgtcgt tggtgcgcga agacacctcg cgaacgctac ctaaacgtat tacctggttg
    10921 ctggataacc aaaaaagact tgtcgtacct gactgggaat catcggcaac ctgtatctcg
    10981 gcaggattat gcatagggat ggtgccaaca catttcgcca aaccgtggct taatgagggg
    11041 aagtgggtag cacttgaact ggaaaacccc ttcccggatt cggcatgttg tctgacatgg
    11101 cagcaaaatg atatgtcgcc tgcgttaacc tggctgcttg agtatctcgg cgatagtgag
    11161 acgctgaata aggagtggct gcgggagccg gaagagactc ccgcaacggg tgattaacga
    11221 cgatagtcgc ggaacgggcc gtcagccacg gagcggcgtt caatcaaacg cggatgcact
    11281 tcaatggact gcggttcttc acgtttgttg acgatacgat ccaacagcat gttgaacgct
    11341 gtttcaccca gcgaatcttt tggctgatgg atcgtggtca gcgccggcgt aaaatagcgc
    11401 gcgttgcgca cgttatcata accgatcagc gaaacatcct gcgggacgcg caggcccatt
    11461 tcatcagcag cacaaagtgc gcccattgcc atgatatcgc caccacagaa gacggcagta
    11521 gggcgatgcg gctgcgacag gatttgctgc atggcgcgat aaccggattc aggttcaaag
    11581 tcaccctgca caatccagct ttccggcacc ttgatcatcg cttcttccat cgccttcata
    11641 aaaccggcaa ggcggcctgc gccggtgtta cgttccagcg ggccggggat gacgccgatt
    11701 tcgcggtgac cgcgttcaat cagataacgc ccggccatgt agccgccttc gaacgcgtta
    11761 tcaatgaccg catcggtgaa gtcagctttt gcttcacccc agtccatcac caccattggg
    11821 atatggcgat actcttccag catcgccagc aacggctctg ggtactcaga acacatcacc
    11881 agcagaccat cgacgcgttt ttgcgccatc atcgacagat aagcccgctg tttctcaaga
    11941 ttgttccacg cattacccag aatcagggtg taacctttct ggaagcaatt tttttcaact
    12001 gcttcaatga tctcggcaaa ataggccgct tcgctgctgg tcgccagcaa accgatagac
    12061 ttggtgtggt taaccttcag gctacgcgcc accgcgctag gggagtagtg taattcttta
    12121 atcgctgccc acacggcgtt gcgcgtttct tcagcgacga aacgtgtttt gttgatcacg
    12181 tgtgacacag ttgtagtgga aacgtttgct cgtttcgcta catcttttat tgttgccatt
    12241 ccatttcact ccagacccta acttcagctc ctgcaaaatc gcaaggtaaa cgtttgcctt
    12301 tacacacctt acgcacaaca aaagtgttgc ggtacgccgg aaaaacgaca cggagcgtca
    12361 ggaaggggtc aatggccagt acgcaaatag tgtaagcgtg gaattttggc cgatcttgac
    12421 gaaaagggga agtggtaaaa acacttatca ttataaatga cgggagattt tttcatcaca
    12481 gtgtgtaaaa atgcgtaata tcattttttg tgtggtaata aaaaaggaga aaacttgatg
    12541 agcaccgacc ttaaattttc actggtaaca acgattatcg tcctcggttt gatcgtagcc
    12601 gtgggtttga ctgccgcgct gcactgattt cctctcgagg gagcgcgttc tccctcgcat
    12661 ctccttcctg tttgttagca aaatggcaaa agtgttttct ctttttgtta ttttcatttt
    12721 tttgtgattg ctgtcatgct ttcgccactt taatgaatat cgcgtaatga cgcgaagagg
    12781 tggagataag gcatgaaaat taactatccg ttgctggcgc tggcgattgg cgcgtttggt
    12841 atcgggacaa cggagttctc gccaatgggc ttgttgcccg tcattgcgcg cggtgtggat
    12901 gtctcgattc ccgctgccgg aatgttaatc agtgcctatg cagttggcgt aatggtgggc
    12961 gcgccgctga tgacgcttct actttctcat cgtgcccgcc gcagtgcgtt gattttcctg
    13021 atggcaattt tcacgctagg caacgttctt tccgccatcg cgccggatta tatgaccctg
    13081 atgctttcac gtattttgac cagcctgaat cactgagcat tttttggttt gggttcagtc
    13141 gtggccgcaa gcgtggtgcc aaaacataaa caggccagcg cagttgccac tatgtttatg
    13201 gggttaaccc tggcaaatat cggtggcgtg ccggcggcga ccaggttggg tgaaaccatc
    13261 ggctggcgga tgtcatttct ggcaacggcg gggctgggag tgatttcaat ggtaagtctg
    13321 ttcttctcat tacctaaagg tggtacaggg gcacgacctg aagtgaaaaa agagctggcg
    13381 gtattaatgc gtccgcaggt gctgtctgca ttgctgacga cggtactggg agctggtgca
    13441 atgtttaccc tctacaccta tatctctccg gtactgcaaa gtattaccca cgcaacaccg
    13501 gtgttcgtca cggcaatgct ggtgctgatt ggtgtcggat tctctatcgg taactatctc
    13561 ggcggcaata acggcacgtt gaaaggcttt ttgttgctgc tgatggtgat tatgctggca
    13621 atcccgttcc tggcccgcaa tgagttcggc gcagctatta gcatggtggt gtggggcgca
    13681 gcaacctttg cggttgtacc tccgttacag atgcgcgtga tgcatgtcgc cagtgaagcg
    13741 ccaggtctgt cttcatcagt caatattggt gcctttaatc ttggaaatgc gctgggagca
    13801 gctgctggtg gtgcggtaat ttccgctggg ctgggataca gctttgtgcc ggtgacgggg
    13861 gcgattgtcg cgggactggc attattgctg gtgtttatgt cagccagaaa acaacctgaa
    13921 acagtttgcg ttgctaacag ctaattaaac aaaaacgcag aaggataacc ttctgcgtta
    13981 cagttgctga caacgtgcga tttatcttgc cgaatgcgct gttaacgcct catccgcgtt
    14041 ggcatggtaa gcgaagcgca tcaggcaatg ctgcatttgc catcagttat tatgcagcga
    14101 gatttttcgc tacgaattcc cagttcacca gcgcccagaa gtgctccaga tagccaggac
    14161 gtgcattgcg atagtcgatg taataagcgt gttcccagac atcaacggtc agcagcggag
    14221 tcgcatcggt ggtcagcgga gtacccgcgt tagaggttga aacgatagcc agtttgccat
    14281 cgctgttttt caccagccag gtccagccag aaccaaagtt tttgatcgct gcatcagtaa
    14341 actgcgcttt gaaatcggca aagctgccaa aagatgcggc gatagcttca gcgacttttc
    14401 cagtcggttc gccaccggcg ttcggtgcca ggcagttcca gtagaaagta tggttccaga
    14461 cctgagctgc gttgttgaat acgccacctt cagagctgcg aataatctct tccagtgatt
    14521 taccttcaaa cgcggtacct ttaatcaggt tgttcaggtt agtgacataa gtctgatgat
    14581 gcttgccgta gtgatactcg atagtttccg cagaaatgtg cggtgccaga gcatctttag
    14641 catatggtag tgcaggtaat tcgaatgaca ttgctactct cctttattat taatttgcat
    14701 acgtacaata gccttattgt gcgtatggat agggtagcaa attaaaaggt gagacaaaag
    14761 atgaacttac cctgttgccg taacaacagg gtaaagtttt agcgaagtgt ttttggggtc
    14821 attacccgac gagcgccaac atagtggcgc tgccagtagt cttcactgag agaagtgatt
    14881 tgaatttcct gacctgtgcg cggtgactga ataaatttgc cgttgccgac atacacgccg
    14941 acatgatcgg ctgtaccgcg tccctgagta cggaaaaaga ccaggtcgcc gtttttcagc
    15001 tcactacgtt cgataggtcc tgcatcacgc aggtgataca tttcattcgc cgtacgcgga
    15061 atacgaattt tcaccaaatc tttataagcg taataaacca ggccgctgca atcaaaaccg
    15121 gtacgcggtg agctgccgcc ccaacgatat ggcttaccaa tttgctgcat cagtttattc
    15181 attgccactt tcgtggcttt ttgcaccttc gctttgtgcg cgtcggcaag ggttcctgac
    15241 gcggcatttt tgactttcac acaatgcgat ttatagccct tacgtttggt acacttttcg
    15301 gtaaagctgg ctgaggccgt tttattgacg gcgtgtttgc tgcgagtttt ggcgttttta
    15361 gaagcggtaa tggaagattt tttaacggaa gaagcggttt tgcttttggt cgtactttta
    15421 ctggcggctt ttttcgctgt tttctggctg gctttctggg tctttttggt ggttgccgtg
    15481 cttttctttt tatctgcttt tttggtgata tgagcggtag cagaactctc cctggcttgc
    15541 tttgaagcat gggccatagg cgttaaaggc agggtggtaa aaagtaaagc acagagcgtg
    15601 atcgaaatac ggtttatccg cgccactgag cgtccctctg ttaaggcagg tctatcatgc
    15661 ctgcgtctga tttacaaaac ttgatgattc taatgcatag aagaagagaa agttaatagc
    15721 ttttattgtt aaaaaatacg tttcgttggc gagcgcaaaa aaatgcagca gtgaaagcta
    15781 aaaatggtta taaatacgcc agtttgccgc gcatcagaga agaaacgaat atttgaaatg
    15841 caacaaatag agcaaaggga taaaatagca aaagcgtgat gaaaatgtta ttttccgatg
    15901 ccgcactgac ccgctacaat gtcagattac tgccgtaaag aagtttgagg aagcaagaca
    15961 atgagcacca ctatcgaaaa aatccaacgc cagattgctg aaaacccgat cctgctgtac
    16021 atgaaaggtt caccgaaact gccgagctgc ggtttctctg cccaggcagt ccaggcgctt
    16081 gccgcatgtg gcgaacgttt tgcctatgtt gatattctgc agaatccgga cattcgtgcg
    16141 gaactgccga aatatgctaa ctggccgacc ttcccgcaac tgtgggttga cggcgagctg
    16201 gtcggcggtt gtgatatcgt gatcgaaatg tatcagcgtg gcgaactgca gcagctgatc
    16261 aaagaaactg ccgctaaata caagtctgaa gagccggacg cggaataatc cgtcctgcaa
    16321 tactgacgga tgcgaacggg catccgtcag tcctttatcc ggtgcgccgt aaaaccccat
    16381 ccttcagggc ggggatataa ggcgcggttt tccacctgac caggtgttta ctgttgttca
    16441 atgtattggc ggatgacgga tattggcgca cctccgcagc tactggcaaa atagccagga
    16501 ctccacaaaa cgcctttgta gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt
    16561 aacctacccg atacgccttt gaggctgttt accagactgg atatcgccag tttgggaggt
    16621 taattaatta acaaatggac gtgatctggt tcgccatcca tttcaaccag ttcagcttca
    16681 aaataagcac atacatttga aaagtaagtg cgtagttttt ctgtcgcgtc atggtcaaaa
    16741 atctggcgtc ggtatctggt gacaaagacc aggtgaacat gcatcaggaa aacacaatgc
    16801 cttccacgcc ggatatcggt ttcttttttc acagaccaaa gtatcatttg acctgtgaaa
    16861 cgattacagg catttaaatt ccagttaaga cccggtggtc aacaggagcg tgaaatgagg
    16921 cgcttcgcag gcgcatgtcg tttcgttttc aatcgtgctc tggcgcttca gaatgagaat
    16981 catgaggccg gtaataaata catcccttac gggaaaatgg cttcctggct ggttgagtgg
    17041 aaaaacgcca ctgaaacgca atggcttaaa gattcgccct cacagccatt gcaacagtca
    17101 ctgaaagacc ttgagcgggc ttacaaaaac ttcttccgga agcgggctgc ttttccccga
    17161 ttcaaaaagc ggggacagaa tgctgcattc cgctacccgc agggcgttaa gctcgatcag
    17221 gaaaacagcc gtatttttct gccgaaactt ggctggatgc gctaccggaa cagccgtcag
    17281 gtcacgggag ttgtgaaaaa tgtcactgtc agccagtcct gcggtacgtg gtacatcagt
    17341 attcagacag aaagtgaagt atcaactccg gttcaccctt cagcatcaat gatcgggctg
    17401 gatgctggcg tggctaaact cgccacgctg tcagatggca cagtctttga gcctgtaaac
    17461 agttttcaaa aaaaccagaa gacgctggcg agacttcagc gacagttaag ccggaaggtc
    17521 aaattcagca acaactggca gaagcagaaa cgcaaaatac agcgactgca ttcctgtatc
    17581 gcaaatatcc gcagggacta ccttcacaaa gtcacaacga ccgtcagcaa aaaccacgca
    17641 atgatagtca ttgaggattt gaaggtcagc aacatgtcaa agtcagcagc gggtacggtc
    17701 agccagccgg ggcgcaatgt ccgggcaaaa tcaggtttaa accgttcgat actggatcag
    17761 ggctggtatg aaatgcgccg ccagcttgag tacaagcagc tctggagtgg cggtcaggtg
    17821 cttgctgttc cgccagcgta cacaagccag cgttgcgcgt gctgtggtca tacagcgaaa
    17881 gaaaaccgcc tgtcacaaag taaattcaga tgccaggtat gtggatatac agcgaacgcc
    17941 gatgtaaatg gcgctcgtaa cattttagcg gcggggcacg ccgttcttgc ctgtggagag
    18001 atggtgcagt caggccgctc gttgaagcag gaacccaccg aaatgattca ggcgacagct
    18061 tgaacgtagc agggaaccac gtctttcagg gcgtggagga tgtcaactat ccccaatcca
    18121 gcccttgtgg cgaacttgaa aatcccgcct cacgcagcag cgtaaacatc ggcgtttgcc
    18181 ggaccggaag atcattcact tctgttagcg taaagcgcag ccttggttcg cgacgcagtg
    18241 cggtggtcag cgcctggaaa atctctgggg cgagtaattc ctccttttcc tgccagacca
    18301 gcattttttt gccgccttgc gccagataga gtaacaattt gccgccagaa acgaccacca
    18361 gcgcacccgc ccgacgcgtt ggtaccagcg ctgccggatg tgcaggccag ggcagaagat
    18421 ttccccacac attagccgga tcgttggcgg agagcgccac tggtgtatag tggcgtgttt
    18481 gcgtggcttg tgtcgccaga tcgcgcaatc ggtcaatagt cagacgttca gcgaattgcg
    18541 cgccacccag accttctaca aaacgacctc gcataattcg cccggagtct tccatacttc
    18601 gacaaagtgt ttgcatcgat ggaaacccgc cagggatatt ttcggctatc accgcctgac
    18661 gactgatgat gccgtagcgg tcgagcatat tttccgccag cgccagcatc ctttcggtat
    18721 cgtttagtgg ctccacctgc aataacgacc agcgtccagc cagatttggt gtgttgtaag
    18781 atacccgcgg cgagacgggt tgtgcataga caggacgccc acgacgagcc cggtgactgc
    18841 ggcgagttga ggtgcgtgcg ttggaactgc tgcgggtgag ggcgcgtaac ggtgcccaaa
    18901 tatcgctggt aatgacgcct tgccagacca gcgcccataa cgcctcttgc agtgccgagg
    18961 aatcaaccga ttcgccgatt ttatcgcgta tccgttggct gatttgttgt gcaaaccagg
    19021 ctcctccgtc agccagaaca gcgattatcg cttgctgcag cgccgaacga ttcgcctgat
    19081 ccgcttcggc gggagtgaac gattctgcgg catatttctg tagatgcagt gccaccaggc
    19141 cgtcatcttc acccagcttt tttttgtccc gaccagataa ccgctccggt tgccagtaat
    19201 tcatcgagca tttctggcga atagtcgcgt acgcgggcag gcaggatctg gctttcccag
    19261 agtgaggcgg gtaaaccgac tccggcaagc tgttcgatca cccgcatcac gccatctacg
    19321 ccctcataaa cgcctggcga tgttgaggca aagagcgccg ggctaccatc ggtggcgggt
    19381 aatacgccct gacgttccag cagcaatcgc gcataggtcg tggctgcaac gggacgcgtc
    19441 gcttctctgg cggcttgcag cgagcgcaaa cgcagacgac gaaatacttc atcgctcacc
    19501 cagatgtctt gttgcagatt catcaccaga ccctgttcac gcagttgctg aagctgctct
    19561 tcgacaatgg caataccgag actaaactca tgagccagtt gttcagccgt gaccagagca
    19621 tgagcccgga gatagcgcag aaagaggtcg cgaagcgggt aactgactct atgtaaataa
    19681 atctctggca atgactctgg tagtcgtacg ccgagggcat cacgcagcct ggcggcatca
    19741 tccatacagg caagacgctc ctgtccgcta atcatcgcag ggaagattcg ttttactgca
    19801 agaagatttt ccagatacga cgcaacctct tcactgctgc ctgtatgccg ttgcgccagg
    19861 tcttcaacgg tcattggccc cagttcgcgc agcaggtcga acaatccttc ttcacctttc
    19921 gctcttctgc caggagccag tcgttgcaac tcttcttcca cctggcgaat gacctgcggg
    19981 tcgagtaatt cccccggatc gacctgtccg agtagattgc gcagtaactc gctgtccagc
    20041 gacagtacgg atgcccggcg ctctgccagc ggggcgtcgc tctggtacat aaattccgcg
    20101 acatagccga acaataaact tgtggcgaaa ggcgagggcg tagtggtcgt tacatcggat
    20161 atttgaattt cgccaccgtt caggcgacgc atcaaacgtt ccagtgcggg aagatcataa
    20221 acatcttgca gacattcgcg tagggtttcg agaatgaccg gaaaatccgg atatccctga
    20281 gcgatttcca gcaactgact ggcgcgcagc cgttgttgcc aaagcggggt gcgatggccc
    20341 ggcgtgcggc ccggcattaa taatgcccgc gcggcgcatt cgcgaaaacg ggcggcgaaa
    20401 agtgccgagc tgcctaccgc ctcgcggaca atttgcagca acttttctgg ttcaaacaaa
    20461 aaaatcgcgg cgtcgggcaa tttaccatca gtgtcaggaa tgcgcgcaac aatgccgtca
    20521 tcactggcga ccacagacgc gtcagcgccc catagggcat gtattcgccc ggcaatcgcc
    20581 agcgcccagg gttcatgcac ccgtcttcca tagggagagt gcaaaataat acgccagtcg
    20641 ccaatttcat cacggcaccg ttcgaggacc agatggcgac tccccggaac gatgcccgtc
    20701 gcgttgcgtt gctcgtcaat caacccctga atattggcgt tcgtattttc ttctgccagc
    20761 cacgggggaa tagtcccgga aaagaacgcg ccatccgcca gtaaatgaag aaaatcgccg
    20821 atcatctcgc ctaattcagc cggacgtccg ttaccttcac cacgccagaa ggggagctgg
    20881 gcagaacgac ccggagcagg agtcacaatc acctgatcgc gggtgatttg ctggatccgc
    20941 catgaggtag cgccgagagt gataatgtcg ttcacccgcg actcatatac catctcctca
    21001 tccagttcac ccacccgccg cgaaccggcc ttttcttcac cttcgggtaa taacacgcta
    21061 tacatgccac gatccggaat ggtgccgccg ctggtaaccg ccaacaattg agcgccaggt
    21121 cgggcggtca atatcccggt ctccctgttc cagaccagtt tggggcgaaa agcagaaaaa
    21181 tcgccagagg gatagcgccc ggaaagcata tccagcgtgg cgtcaaaaac acgtcttggc
    21241 agatctttcc acggtgcggc acggcgtacg cgggagtacc attcgtctac ctgtaatgca
    21301 tccatcgccg cggcggcaac ggtttgctgc gcaaggacgt cgagaggatt atgcggtggt
    21361 gtcaggtttt ccagcctgcc tgcgaacata cactctacaa tgactgcgga atcgactaaa
    21421 tcacgccggg tacgggggaa aaacagccct ttagatacac cgccaacctg atgtccggcg
    21481 cgaccaatgc gttgtaaccc actggcaaca gaaagcggcg ttgcctcctg aatcaccaga
    21541 tccaccgcgc ccatatcaat ccccagttca agactggagg ttgcgaccac gcagcgtaat
    21601 tcacccgatt tcagcgcctg ttcggtgatt gctcgttgtt ctttagagac ggagccgtgg
    21661 tgtgagcggg caataaaaac gtcgctactt tgtacacggt tagaggttgc gccggaggtc
    21721 gactcgaaat gggccgcatc aacggcgata gacggggaac gctgtaagcg tgcggcgtaa
    21781 agctcattta atcgtgccgt cagtttttcc gccagcccgc gtgaattagt aaagacaatg
    21841 gtcgagcgat ggcgcaacac ttcatcaagg atacccgttt caatatatgg ccagatggag
    21901 ccttcccggc cggcatggct gtcttcgccg gtgccgctgg cgaccgatga gacatcatcc
    21961 atattggcga ccggtacgac aattcgtatc tgcggatggc gcattgcggg cgggttgact
    22021 accgtaaccg ggcgatcgcc accaagaaat gctgccacat cgctggctga gcgcacagtg
    22081 gcagaaaggc caattcgctg tgctgaggtg tggagcagcg catcgagccg ctccagactt
    22141 aacgccagat gcgcaccacg tttactgccc gctaccgcgt ggacttcatc aataattacc
    22201 gtttcgacgc cgtgtagcgt ttcgcgcgcg cgggaggtca gcatcagata gagtgattcg
    22261 ggtgtggtga tcagaatatc cggcggatta cgggtgagtt tgctgcgttc ctgtgcaggc
    22321 gtatcgccag tacggatccc tacgcgaaga ttgacttccg tttcgccgcg ccgccgccgt
    22381 tcatcggcaa tacccttcaa cgggatctgc aagttgcgct gaacgtcggt gcccagggct
    22441 tttatcggtg aaatatagag gatgcgtgag gttttacgct tatgcgcctc gcgggtatct
    22501 tcgccgcctt cgcggaagag ccgatcgagg gcgtagagaa atgctgccag cgttttcccg
    22561 gagccagtcg gtgcaatcac cagcgcatgt tcgcctcgcg ccgccacatg ccaggtttgc
    22621 gactggacag cggtcggctg tttaaaggcg cgaagaaacc agtcgcgggt cgccggtgaa
    22681 aacacgtccg gcaggagcga tgaagggtct ggattatctg ccattaacgc tattccccgt
    22741 gaatatcatg cttgatgcgt ggaatagcct cactatagat gcctgaatca ttgcatgtca
    22801 tcaggcatcg actcgattac acctcttcgg cggcaggtag cggccagcct cccagacgtt
    22861 tccagcggtt gacgatttca caaaacagca cagcagtgcg ttcggtgtcg tacagcgcgg
    22921 agtgcgcctg ggtgctgtcg aagtccatgc cagcggtctg gcaagccttt gacaatacgg
    22981 tttgtccgag tgccagcccg gccagtgcag cagtgtcaaa agtggcgaaa gggtggaacg
    23041 ggttacgttt cagcgaggcg cgttctgcgg cggccatcat aaagctgtga tcaaaattgg
    23101 cgttgtgcgc caccataatg gcgcggttac agccgctcgc tttaatacct ttacgtacaa
    23161 ctttaaaaat ttcgtgcagc gcctcgtatt cgctgaccgc gccgcgatcg ggatcgttcg
    23221 ggtcaatgcc gttgaaggcg agggcttctg gttgcaaatt tgcgccgaca aatggttcga
    23281 cgtggaaatg taatgtggtg tccggcatca gccagccttg ttcatccatt ttcagggtga
    23341 tggcggcaat ctcaagcagc gcatcggttt tggcgttaaa tccggctgtt tcaacatcga
    23401 tcacaacagg ataaaaacca cgaaaacggt cgcacagacc ggtaagttga gcgttatcgg
    23461 acatctgatt ctcttaatac ggataaattg cagcgcgcat tatgacaaat attacttgtc
    23521 gatgcagtaa agatgcgggc gcgatgagtt cacgcccggc aggagattag ttgcccagac
    23581 cgcgaccggc gtctttctct tcgattaact caattttgta accgtccgga tcttccacaa
    23641 acgcgataac cgtagtaccg ccttttaccg gacccgcctc acgggtcacg ttacccccgt
    23701 tttgacggat tttttcgcac gcttcagcgg cgttatctac gctaagcgcg atgtgaccat
    23761 aagcagtgcc gagttcgtat ttatccacgc cccagttgta ggtcagttca atcaccgctt
    23821 cttcggtttc cgggccgtag ccaacaaacg ccagtgagta tttgtattcc gggttttcgc
    23881 tggtacgcag cagtttcatg cccagcactt tggtataaaa atcgatggag cgttgcaaat
    23941 cgccaacgcg cagcatggta tgaagaagac gcatcttttt atcctcaaaa tggctaacag
    24001 attcaaaatg ttgttttagt atagcggcgg ctttacgccg ctcgcaatgt tggattacaa
    24061 cgtcgggtaa tcggtatagc cttccgcgcc gccaccgtag aaactttcgg cacgctgtgg
    24121 gttaagctca gctttgagct gcaagcgggc gaccagatcc gggttcgcaa tccagtcacg
    24181 accaaatgcc accgcatcaa ttaacccttt gccgatcagc gtttcagctt tttctactgt
    24241 gtatgcacct gcgccgataa tcggaccgtg gaaacgggcg cgtacttttt cgcggaacgc
    24301 atcagtatac ggttcacccc ccgcccaatc tggttctgac atatgcagat aagcaatgcc
    24361 gcgtttaccc agttgttcaa tcagatacag tgcatcggct tcttcattcg ggccgttatc
    24421 tgtgttctgg aaagtaccga ttggcgaaac gcgaatgcca atgcgatcgg caccccattc
    24481 ttcaatcccg gcatcgacca cttccagtac caaacgtgcg cgattttcca cgctgccgcc
    24541 gtactgatcg gtacgatggt ttgaagaagg ggaaaggaac tgatgcagca aataaccgtg
    24601 agcagagtgg agctctacca gatcaaaacc ggcttcacgc gcgttagcaa tggcctgacg
    24661 gaaatcattg acgatacccg gaatctcttc cagttcaagc gcacgcggca tggatgtttc
    24721 aacgcggatc gcccgaccat tttcatcgcg cagagaagta cgtgttcccg cgctaagtgc
    24781 tgaaggcgct accggtgcct gaccgccagg ttgcaggctg gcgtgagaaa tgcgtccggt
    24841 gtgccacagc tgcaaggcca tatgaccatt ttcagcatga acgccagcgg tgattttttt
    24901 ccatgcggca atttgctccg gactatggat gccaggcgca cctgcatatc cttttgcctg
    24961 ggcagaaatt tgcgtggctt cactaataat caaaccggca ctggcacgtt ggcgatagta
    25021 ttccgccatc aacggggtag gaatgtcacc cggttcaata ctgcgcagac gcgtcagcgg
    25081 tgccataaaa atacggtttg ccgccgtgat cgcgcccact ttcagtgggg aatacagttt
    25141 ttcagatgac ataaaggctc ccggtcgtct ggtgataaaa aatgcctgct aaacggcagg
    25201 cgtcgcaata atgtttttta catgggccag cgcgttttcc agtggctcga aactgcgcga
    25261 aattttggcc tgcagattcg cgccaagcca tagtgcgtaa agcacttgtg cctgttgtag
    25321 cggttcgcca caaaaggtta aacaatggtt ctcacggcca ttttccagag cctgcgagag
    25381 cagggcgatc acgccgcgag cacctttatc catcgcgctg cgcatatctt ctgacagatc
    25441 gcacacttcg gcagagagtt ttactgtcag gcaaccactg atggttccat gttggcaaaa
    25501 ctggttcagt gtttgctggt aataagccag tatgcggtcg cggtagttac cttcgccgga
    25561 ttgcagcaac tcagccagtc gctggtgata tgcggcgtaa tgacgctcaa gcatggcaac
    25621 gccaaacgct tctttagagc gaaagtagtg atagaaggac cctttcggca cttcagcggt
    25681 ttttagtaat tcgcttagcc ccatcccggt gaatccacgt tgcaggcaaa gttgctcgcc
    25741 cgtcgccaag agatgttcgc gagtatcatg ttcggtgtgt ttgttcatgc gttggagtgt
    25801 agtagaccag tcggtctagt gcaagcaaag ttgctacttt ggacattaac agcttcaata
    25861 atggtaataa tcttatctga ggagggcgag gtggcggagc aattagagtt ctttcccgtc
    25921 cagagcccgt gccggggaat ttgccagtct gacgaacgcg ggttttgtcg cggctgtttt
    25981 cgtagtcggg atgagcgttt taactggaat aaaatgagcg atggcgaaaa acaagaggtg
    26041 ctgcgccttt gtcggcagcg attaatgcgc aaattacgcg caaataagcc agcgtcatca
    26101 gacgaaccgg agcaaccatc actcttttga acctaatctt gcgtatactc ctctcatatc
    26161 tgataagagg aagcgattat ggttcagcgt attactattg cgccggaagg cccggagttt
    26221 tcccgttttg tgatgggcta ctggcgattg atggactgga atatgtccgc ccgccagctg
    26281 gtcagtttta ttgaagagca tctggatctc ggcgtgacca ccgtggacca tgctgatatt
    26341 tatggtggct atcagtgcga agcggcgttt ggcgaggcac tgaaactggc acctcacctg
    26401 cgtgaacgga tggaaatcgt cagtaaatgc ggtatcgcga cgaccgcgcg tgaagaaaac
    26461 gtcattggtc attacatcac tgaccgcaat cacatcatta agagcgccga acagtcgcta
    26521 attaatctcg cgaccgatca tctggatctg ctgttaatcc accgaccaga cccgttaatg
    26581 gatgccgatg aagtggcgga cgcgttcaaa catctgcatc agagcggcaa agtgcgtcat
    26641 tttggcgtat cgaactttac gcctgcgcaa tttgccctgt tgcaatcacg tctgccgttt
    26701 acccttgcca ctaatcaggt ggaaatatcc ccggtgcatc agccgttact gctggatggc
    26761 atgctcgacc aactacaaca actgcgtgtt cgtccgatgg cgtggtcctg ccttggtggt
    26821 ggtcgtctgt ttaatgatga ttatttccag ccgctgcgtg atgaactggc tgtggtggcg
    26881 gaggagttaa acgcgggctc gattgaacag gtggtttacg cctgggtatt acgtttaccg
    26941 tcgcagccgc tgccaattat cggctcaggt aaaattgagc gcgtacgggc agctgtcgaa
    27001 gcagaaacac tgaaaatgac ccgtcaacaa tggtttcgta tccgtaaagc tgcactgggg
    27061 tacgacgtac cgtaagcact tttaggaata gccgccgttc aaaaatgtgt cactggttta
    27121 cacttattca ggaatgcaca atgaacggag gtcctatgaa acgttttagt ctggccattc
    27181 tggcgctggt tgttgcgacc ggcgcacaag ctgccagtga aaaagtcgag atgaacctcg
    27241 tcacgtcgca aggggttggg cagtcaattg gtagcgtcac cattactgaa accgataaag
    27301 gtctggagtt ttcacccgat ctgaaagcat taccccccgg tgaacatggc ttccatattc
    27361 atgccaaagg aagctgccag ccagccacca aagatggcaa agccagcgcc gcggaatccg
    27421 caggcgggca tcttgatcca caaaataccg gtaaacatga agggccagaa ggtaccgggc
    27481 atttaggcga tctgcctgca ctggtcgtca ataatgacgg caaagctacc gatgccgtca
    27541 tcgcgcctcg tctgaaatca ctggatgaaa tcaaagacaa ggcgctgatg gtccacgttg
    27601 gcggcgataa tatgtccgat caacctaaac cgctgggcgg tggcggtgaa cgctatgcct
    27661 gtggtgtaat taagtaatta cgaggccagc gtaccttgcg gtggtgcttg ctcaagttgc
    27721 gaaagcgagc agtacagccg ccagacaatt gccgccagct cacgggcggc aggttgatga
    27781 tgacgggcaa ggctgtcgca aatccgctgt aattcttcaa gtgtggccgc cagtgatttt
    27841 tgctgaacgc cacgctcact catcactccc cgcaacagtg aaatacagtt atcccgtact
    27901 cgcgataacg gatcggaacg cgattcccag tcgcgcaatt gccagacaac atgagaacag
    27961 ttcagcagca ctacacccca gcgtaataac caacggcgag ccagcgcatc ctggctgtta
    28021 ctcaactgac tgacgtgatg ataagtgagc gattcaaatt cgctttcact cagtgttgga
    28081 tggcggctta gctgatcgac aaaatcccgg cgcagcgcgc gaatatggcg gcggctttta
    28141 cgagcatccg atcctggacg cagaatggcg aacgctagcc aagccagcgc aacgccaacg
    28201 atttttgcca gattatcgtt aagaaaatca gcaaaatcat acaccggggg attagtgaca
    28261 gcgataaacg aacccataaa aacaatcagt tgcccccaca gtgcggcaaa tttgggcatc
    28321 tgcaatttaa gaagctgcat tgtcgccagc agtggaaaga gaaacagtaa aaattgccac
    28381 agatcgctaa tctggaccat caggccaaat ttgaccacaa agctgaatag cgaaagtaac
    28441 accagcgtgc gcatcagcag tgacaacgac ttaaacggtg ctgcgacggc ggagtagagt
    28501 acgcagctaa ttgctgccag cgttaatgca ttggcaccgg catcccattg cgatgcaata
    28561 ctccatgcgc caatcatcat caacgtacaa aatgtacgca gcccgctcca catagcttcg
    28621 gcgttgtcgg tatgacgagc cagagcggat gtccgtgcga gtctggcatg atcatctaca
    28681 ccgctttgca gacgatgtaa ttcctgacta ctttgcagat aaaggcggca aaaataacgt
    28741 agtcgctgcc agaaggcgac gtgccggtag tcggcgacgt tggtcgggcg tagcggggcg
    28801 ataatacgtg cgacggtgta agcatctgtt tgcgaactgg cgagcgccgt cagtaattgt
    28861 tcgagaattt ctcgcgtggc acctggcggt gagggccagt tgagcaacat acgtcgcagg
    28921 ctggagatga cactggtcat acgtaattgc tggtggagta gcgcattaag gcgcgcgttt
    28981 tgctggcgaa aacgatagtg gctccagaaa gcctggatac gcagcaaatt catggtcagt
    29041 atctgcccaa tcaccccctc atgtgctgca cgaatggcat cgtttgtttc aggctgccag
    29101 agtaaactgg catgttccag taatcgggcg tgcatgtttt tcaatgcagt taaaagggca
    29161 gtagcatcgg aactgctagg caggatcatc atcatcatgc cgccgcacaa aataccgact
    29221 atcacctcgc aaacgcgcgc ctgagcgata tcccacagct ggctggcctc agtaatatta
    29281 accatcggaa aggcgatgat ggcagccgtg tagcccgcca gttgaaatgc atacgcgacg
    29341 ttattcgtga agtgcgcaca ggcctaggta caaaagccaa gccacgccga catgctcaat
    29401 agaaaaaacc acggctcatt gagcgtatgt ccggcaagaa gcagtgccgc aatggctccc
    29461 agtaaactgc cagcgatgcg tccgaggctt ttgctgatga caccgccaac ggtgggaaag
    29521 ctaaccactg cagccgaggt catcgcccaa tagggttcat ccagattcaa ataataggca
    29581 accgtcagcg ccagacacat ggcaatggta ttgcgtaacg cataacgcca ttgcgccagc
    29641 gtggccctga accagcgcaa agcggacggg aacgcgttgg gcgagacgaa cccaggggac
    29701 gttaggtttc acatccggaa taaggcttga gtcactttca acgctctgat cataaatcgc
    29761 ccgaccgata ctggaaacgt gaccctgtaa cgttttgtta tcactataga gggtaatttg
    29821 tgctggtgca ccttcgcgga tatggcgcaa cttggtttct tcaaaataac caatgacgta
    29881 aaacgagtgg ctgtcgacaa gggcaaacag cggtttcccg gtgtcagcgt aatcgccgat
    29941 gcgggtagtg aggttagtca cccatccact caccggggcg cgaatttccg tttgcgccag
    30001 ttgccattgt gtctgtttca gcgtcgcctg tgcggcatta acactggcct gcatcgcttt
    30061 aacattgagg ttagcggtat cgagttcttc ggcaaaaata aaattttgtg agagatgacg
    30121 acgacgattc gcttcgttat tggcttttgc gaggtcagat tgcgctttcg ccagctgagc
    30181 ctgtgcgttc agctcggcga tctgaaaggg cgttttatcg atcgtgagca gaagttcccc
    30241 cgcgttaacc agttggttgt cttttatatt cagctcaaca atacgtcccg acacctgtgg
    30301 ggtgatagaa acctgttctg cgtgtatttt cccgtcgcgt gtccacggag attgcatgta
    30361 ataattccac agccaccatc cggcaagaac cgcaactatc gctacaatga ttgttgagaa
    30421 atacttaatt gttttaattg acatagttac cacgcaatca gtattgccag agcaaggcaa
    30481 acgcaaatcg caaacagcga taaatccatt aacaagggat gccagatgtc accggcgtag
    30541 atccagccac gaagcagacg gtgtacgaca agccagatga cgaatccaaa cgcgaatgct
    30601 ttgaaaaacg gaggaaaata gacggacgca ccgaacacca gatcttgtaa gggcaatcct
    30661 gttgcattga gcataaactt cacaaagtag attcctttac gaccgaatat attgcgttag
    30721 attaataaat attctttaag tgcgaaaaat ttacgcgcaa tttctgaaaa atatgtctga
    30781 cggtaaccaa atgcagcaat acatttgttt tagcaataca attgctgcac actattctaa
    30841 aagccgcata atatcttagc aagctaatta taaggagatg aaattggaat cgccactagg
    30901 ttctgatctg gcacggttgg tgcgcatatg gcgtgctctg atagaccatc gcctgaaacc
    30961 gctggagtta acacaaaccc attgggttac gttacacaat atccatcagt tacctccaga
    31021 ccagtcgcaa attcaactgg caaaagcgat tggcatcgag cagccatcac tggtccgtac
    31081 tctggaccaa ctggaagaaa aagggttaat ttcgcgtcaa acttgtgcca gcgatcgtcg
    31141 ggctaaacgt attaaactga cggaaaaggc agagccactg atcagcgaaa tggaagctgt
    31201 tattaacaaa acccgcgcgg aaatattaca tggcatctcc gcagaggaac tggagcaact
    31261 gattacgctc atcgcaaaac ttgagcataa tatcattgag ttacaggcca aagggtgaaa
    31321 tgaagggggc gtgtggtcag taacctgacc acacgcaaac ttatttagcg cggagaaacg
    31381 gtcacctgac tgccattgct ggccagtacg acacgttggc ccggagagaa acgagtgttg
    31441 ccttgtttct gtaccaccat gatggtatta ccatcgtctt tacgaatttc cagctcgaca
    31501 ccctgcgttt tgttcattgc actctgtacg ccctgaccag ctacgccacc tgcaacagcg
    31561 cctgctgcag tagccagaga acgcccggtt ccgccaccaa cagtattccc caggaaacca
    31621 ccaagaacag caccgccaat tgcaccgata acgttggaat catcaccgcc ctgaatctgt
    31681 accggacgta cgttaacgat ggtgccatag ctgacattct gtacttgttt cgcttcagaa
    31741 gcggtataaa catcccctga cagggtgtcg ttattaacac aaccgacaag agacagacct
    31801 accattgaaa caaccaatac gcgtttaatc actgaaaaat ctcctgttca ccatgaaacg
    31861 ccacgcaagc atccctcatg gtcaaagtat atggcatatg tgaatccatg gttacatgtt
    31921 ctaccaaata tgagcaaatc ataatgaaag taggatcaac caattataaa caaaacattc
    31981 atgatgatta ttgatttgaa gataatgttt tactaccaac ggtaaaaatc ggtcattccg
    32041 catggtattc acgaaaagtt tatgtttgag tggtgcgcaa acgtcagatt gaaaaaggat
    32101 gaactatgaa atcgggccgc tttattggcg ttatgtcagg caccagcctt gatggtgttg
    32161 atgttgtgtt ggcgacaatt gatgaacacc gggtcgcaca gctggcaagc ttgagctggc
    32221 cgatcccggt gtctctgaaa caggctgtac tggatatttg ccagggccag cagcttacac
    32281 tttcgcagtt tggacagctt gatactcaac tcgggcgact ttttgctgat gcggtcaatg
    32341 ccttgcttaa ggaacaaaac ctgcaggcga gagatatagt tgcgatcggc tgtcacggtc
    32401 aaaccgtctg gcatgaaccg acgggcgtgg caccacacac tttacagatt ggcgataaca
    32461 atcaaattgt ggcacgcacc ggaattacgg ttgtcggtga ttttcgccgt cgcgatattg
    32521 ccttgggagg acaaggcgca ccgctggtac ctgcgttcca tcatgccttg ctggctcacc
    32581 caaccgagcg acgaatggtg ctcaatattg gcggcatcgc caatctgtca ctgctcattc
    32641 ctgggcagcc ggttggcggc tacgataccg gtcctggtaa catgctgatg gatgcctgga
    32701 tctggcgtca ggcaggtaaa ccttacgata aagatgccga gtgggcacgg gcgggtaaag
    32761 ttattctccc actgctgcaa aatatgctca gcgacccgta tttctcgcaa cctgcaccga
    32821 aaagcaccgg acgcgaatac tttaactacg gctggctgga gcgccattta cgccattttc
    32881 cgggtgttga tccccgtgat gttcaggcca cactggcaga actcaccgcc gtgaccattt
    32941 ctgaacaagt tttgttgagc ggtggctgcg aacgattgat ggtatgtggt ggaggtagtc
    33001 gtaatccgct actcatggcg cgtctggcgg tattactgct aggcacagaa gtcaccacca
    33061 ccgatgccgt tggcattagt ggcgatgaca tggaagcatt ggctttcgcc tggcttgcct
    33121 ggcggacgct ggcgggatta ccaggaaatc tgccttccgt cactggcgca agccaggaga
    33181 cggtactggg ggctattttc cccgccaact cgtgatatca gaattaactg aattttttct
    33241 tatcgtgtct gccgttacac ttggaagatt aaggagggta tttgccctcc ggaccaggaa
    33301 agtcttcggg atatgactat gaaaaaactg ttaattatca ttttgccggt ccttctctcc
    33361 ggttgtagcg cctttaatca actggttgag cgaatgcaaa ccgatacgct ggaataccag
    33421 tgtgatgaaa aaccgttgac ggtcaaactg aataatccgc gccaggaagt cagttttgtt
    33481 tacgataatc aactactgca tctcaaacag ggcttttcag cctctggcgc gcgttactcc
    33541 gacgggatct atgttttctg gtcgaaaggt gaggaagcga ctgtctataa acgcgaccgc
    33601 atcgtcttga ataactgtca gttacaaaat ccacagcgtt gagatttttc caggggcggc
    33661 gcacaatagc gtcacccact gacaatccgt aaagaaaacc atgtctgata acgacgaatt
    33721 gcagcaaatc gcgcatctgc gccgtgaata caccaaaggc gggttacgcc gccgcgatct
    33781 tcccgccgat ccattaaccc tttttgaacg ttggctctct caggcttgtg aagccaaact
    33841 ggcggaccct accgcgatgg tggtcgctac cgtggatgaa catggtcagc cttatcagcg
    33901 catcgtttta ctcaaacatt acgacgaaaa aggcatggtg ttttacacca acctcggtag
    33961 ccgtaaagca catcaaattg aaaataatcc gcgcgttagc ctgctgttcc cgtggcatac
    34021 ccttgaacgc caggttatgg tgatcggtaa agcagaacga ctttcgactc tcgaagtgat
    34081 gaaatatttt catagccgcc cgcgtgatag ccagattggt gcatgggttt cgaagcagtc
    34141 cagtcgcatt tctgcccgcg gtatccttga aagtaaattc ctggagctga agcagaagtt
    34201 tcaacagggc gaagtgccat tgccgagctt ttggggcggt tttcgcgtca gccttgaaca
    34261 gattgagttc tggcagggcg gtgagcatcg cctgcatgac cgctttttgt accagcgtga
    34321 aaatgatgcg tggaagattg atcgtcttgc accctgaaaa gatgcaaaaa tcttgcttta
    34381 atcgctggta ctcctgattc tggcacttta ttctatgtct ctttcgcatc tggcgaaaag
    34441 tcgtgtaccg gcaaaggtgc agtcgttata tacatggaga ttttgatggc aagcagtaac
    34501 ttgattaaac aattgcaaga gcgggggctg gtagcccagg tgacggacga ggaagcgtta
    34561 gcagagcgac tggcgcaagg cccgatcgcg ctctattgcg gcttcgatcc taccgctgac
    34621 agcttgcatt tggggcatct tgttccattg ttatgcctga aacgcttcca gcaggcgggc
    34681 cacaagccgg ttgcgctggt aggcggcgcg acgggtctga ttggcgaccc gagcttcaaa
    34741 gctgccgagc gtaagctgaa caccgaagaa actgttcagg agtgggtgga caaaatccgt
    34801 aagcaggttg ccccgttcct cgatttcgac tgtggagaaa actctgctat cgcggcgaac
    34861 aactatgact ggttcggcaa tatgaatgtg ctgaccttcc tgcgcgatat tggcaaacac
    34921 ttctccgtta accagatgat caacaaagaa gcggttaagc agcgtctcaa ccgtgaagat
    34981 caggggattt cgttcactga gttttcctac aacctgttgc agggttatga cttcgcctgt
    35041 ctgaacaaac agtacgatgt ggtgctgcaa attggtggtt ctgaccagtg gggtaacatc
    35101 acttctggta tcgacctgac ccgtcgtctg catcagaatc aggtgtttgg cctgaccgtt
    35161 ccgctgatca ccaaagcaga cggcaccaaa tttggtaaaa ctgaaggcgg cgcagtctgg
    35221 ttggatccga agaaaaccag cccgtacaaa ttctaccagt tctggatcaa cactgcggat
    35281 gccgacgttt accgcttcct gaagttcttc acctttatga gcattgaaga gatcaacgcc
    35341 ctagaagaag aagataaaaa cagcggtaaa gcaccgcgcg cccagtatgt actggcggag
    35401 caggtgactc gtctggttca cggtgaagaa ggtttacagg cggcaaaacg tattaccgaa
    35461 tgcctgttca gcggttcttt gagtgcgctg agtgaagcgg acttcgaaca gctggcgcag
    35521 gacggcgtac cgatggttga gatggaaaag ggcgcagacc tgatgcaggc gctggtcgat
    35581 tctgaactgc aaccttcccg tggtcaggcg cgtaaaacta tcgcctccaa tgccatcacc
    35641 attaacggtg aaaaacagtc cgatcctgaa tacttcttta aagaagaaga tcgtctgttt
    35701 ggtcgtttta ccttactgcg tcgcggcaaa aagaattact gtctgatttg ctggaaataa
    35761 tgcattaagt ggaaaggggg agtgagaaat cactccccct ggtttttata cagggaacat
    35821 gatgaaaaat attctcgcta tccagtctca cgttgtttat ggtcatgcgg gtaacagtgc
    35881 ggcagagttt ccgatgcgcc gcctgggcgc gaacgtctgg ccgctgaaca ccgttcaatt
    35941 ttctaatcac acccaatacg gcaaatggac tggctgcgtg atgccgccca gccatttaac
    36001 cgaaattgtg caaggcattg ccattgccgc cattgataaa ttacacacct gtgatgccgt
    36061 attaagtggc tatctgggat cggcggagca gggtgaacat atcctcggta tcgtccgtca
    36121 ggtgaaagca gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga
    36181 aaaaggctgt atcgttgcac cgggtgtcgc agagtttcat gtacggcacg gtttgcctgc
    36241 cagcgatatc attgcgccga atctggttga gctggaaata ctctgtgagc atgcggtaaa
    36301 taacgtcgaa gaagcggttc tggcagcgcg cgaactcatt gagcaagggc cacaaattgt
    36361 gttggttaaa cacctggcgc gagctggcta cagccgtgac cgttttgaaa tgctgctggt
    36421 caccgccgat gaagcctggc atatcagccg tccactggtg gattttggta tgcgccagcc
    36481 ggtaggtgtt ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac
    36541 gctgcaggag gccctggaac atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa
    36601 agcaatgcag gaatatgagc tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga
    36661 acattacttc agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg
    36721 gccgatggct attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca
    36781 gccatacgtt gcataaatgc cgcaatgtgc tctaaccctt ccagattcag tttcaccgcg
    36841 tatgcccagc gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag
    36901 atccagtgct catccttcag tgcctcgttc acatattgca gcttcttatc cagctgcgcg
    36961 cgaactgtcg gtttgtactc ttccggtgta tctgggcgaa acagaggtgt gaaaccttta
    37021 tgcagctcgg tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt
    37081 accggtgcca gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgcaacg
    37141 ccttccgtca gcaaagtacc atcgtccagc agcaatgcag gcacctgccc cttagggtta
    37201 acggaaaagt aatcgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagc
    37261 gtaaaatcct ttccgctctc acgcagggtg atatgggaag cgagagagca ggcgcccggt
    37321 ttgtagaaca atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt
    37381 gctgaacaaa aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa
    37441 ttttacgcta cagtagcttt cgccgctttg tctgcagcgt catcctgcgt catgcggtgc
    37501 agtttaggcg cggtcagcag catcagtact gcaataacgg cagtagcgac accaatctgc
    37561 aagaatacgc gaccatagac ttccagtgac atcagcgggt cggtaacgtt atccggcaca
    37621 gccatcatac ccgcaacata accaccaatc aggtttgcac cggcagtggt caggaaccag
    37681 ctacccataa tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc
    37741 agaccagaga tcatcagttc cccgatgctc tgcaggccat agcttgcgac cagccagctg
    37801 acagacacga tacctgcgtc agacgcgaat ttcgcaccca gcggcagaat caggaacgca
    37861 ccagaacaca tcaccatgcc gattgcaaac ttggttggca tcggcagggt atcacccatc
    37921 ttgttataga tagcggccag aatcggacta ccgatgatga tccagaacgg gttcagtgcc
    37981 tgatactgct caggttctac ggccagaccc agaatggagt gctcaacgtt acgaatcgca
    38041 aagaagttca gtgacgttgg catctggctg tacagcacga agaagatgat tgcttcgagc
    38101 atcaggatga aggcaacgat cattttacga cgcgcagcac ctttcatcgc gaaagcttct
    38161 ttaccgaaga taaccacaat accgaaggca acaacgccca gcgccatacg cgcaacttcc
    38221 tgattgtgca gtagccaggt ggcaacagcg atcagcgcca caacaccaat aatggtcagc
    38281 aacaggttac ggtagttgac tggttcaaag tctggttttg aaccgtactg tttaacccag
    38341 cgttggcaga aggcgaagtt aacgatagtg atcagcaggc ctacaacgct caacgcaaac
    38401 gcaacactcc agccgtattt cgcggccagc cacggggtgg ctatcataga gaagaaagag
    38461 ccgatgttga cggacatgta gtacatggtg aatgcaccgt ccagacgtgg gtcgtttttc
    38521 tcatagcatg tagaaagcag agaagacggg ttagctttaa acaggccgtt accgaccgca
    38581 atagccgcca tacccatata aacaataccg gcgtcgtgac cagaccaggc aaccagagca
    38641 taaccaatcg ccagcacaat agcgccgagc ataattacgc gtttagtacc cagtaccttg
    38701 tcacctaacc agccgccgat agcgaccaga ccataaacca gggcactaaa ggaagagaaa
    38761 agggtgattg aatccgcttc agacataccc agttgtttaa ccaggtaaac agccataatt
    38821 ccttgtaggc cgtaataacc aaaacgttcc cataactcaa tcgagaagat gagatagaac
    38881 gccttcggtt gtttgaaagc gttcaaactg acgctttcag ttggtttttg gtttgcagtg
    38941 gacactttta cctctttttt tacatcccat attaacgggg gggctttctc gcgttatgtc
    39001 taaaaaacat acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat gttcactatc
    39061 ttgcactttc agacaagtcc tttgtaataa tctgttacat ataactgagg cggggaattc
    39121 aaccattccg ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac ggcagatttt
    39181 ttcactgcat tgagaggggg atgatggctt gacggagata tgttctgcta tttagcgttt
    39241 ttcctagtga gatagtccag tttctgaaaa atagccagtg taatgttttg caggtcaata
    39301 tttgttgcgt gtgtaaattt aaatcatttt aacctgattc ttgcaatgtt tttacattgc
    39361 cacagcatcg tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta
    39421 acctgcattt ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc
    39481 attgacgtta aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg
    39541 tgttacccct tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat
    39601 acaagagcca cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca
    39661 atggtggcag tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg
    39721 ttcgacgttt ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc
    39781 gacagcaata gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc
    39841 tacgccgggc agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgttgttc
    39901 cagtaatata cggcaggttt tgatgatatt ttctgctttg ctgttataaa ggccaatcgt
    39961 tttgatatag gttttcaccc cttcaacgcc cagttcaagc atcgctgcag gcgtattcgc
    40021 caccgggtag agtttcgccg tcgccttatt aacactgata tcggtcgcct gagcggaaag
    40081 cagtacggca atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg
    40141 attgttctca cgcaggcgag tgaggatctc cagacgtttt gctttgttca tcagacattc
    40201 cctgtttcac cgtttggcaa tgcacgttcg gcagctgctt cagcacggcg ctttttcatt
    40261 ctttcatcaa tcaggtattt tcctgccagc atcagtccca agccaataaa tgcacctggt
    40321 ggcagcatcg ccagcaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt
    40381 gcccagctac ctaacagcgc atctgcaccg tcaaacaacg tgccattgcc gataatttcg
    40441 cgtagtgaac ccagcacgaa catggcgcag gttgcgcccg taccaattga aaagccgtcc
    40501 agtgccgaaa gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata
    40561 cagttagtga caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag
    40621 gcgttgatca gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga
    40681 atgcggatct cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc
    40741 agtaccagcg tagtcgcaag tcctaaaccc agagcgttag tggcagtgga cgtgaccgcc
    40801 aatagcggac aaaggccgag cagttggacc agtgcagagt tgtttttcca caacccctga
    40861 acaataacgt ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt
    40921 tgtgccggta acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg
    40981 ggagtaatcg tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag
    41041 tgcgcatcat ccgcaccact gatttttttt cccgcaaaat gggtgagcca gtcagaaagg
    41101 cgcaattcga ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca
    41161 agtaccgtgc cattaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct
    41221 ggcgcggttg cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc
    41281 cgatgctcac ctttacctaa ctctggcgca gttaccagat agcaactctg tgcgagcgca
    41341 ttgttatagc gttcggctgg cagcacctga ccaaataacg ccttttgttg cagactggcc
    41401 tgttcagcaa ttgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgtagaaccc
    41461 gctgcaaata gtgccagtgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct
    41521 ttgcgatggc cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc
    41581 gccagcagga cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag
    41641 actaataagc ccgccagcgc gccgaacatc agccgaccac gattggtcgt agaagcagta
    41701 actgggtcag tcaaaataaa gaatgcgccg agcatagtcg caccagacag cagatgaatt
    41761 tgcggtgctg ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc
    41821 gttactaaga agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacacg
    41881 ccaccagcca gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg
    41941 tagatcggat attgcataat ctgttcaacc gaatgacctg cgcggacaga ggttttgaat
    42001 gtatccagcg gtgtcgcctg actaatgcca tcaataccta agcgtagcgt attcatatca
    42061 ccaccactgg cggtatgacc gctgaaaata acctgtatgg cgtcgataaa accagggatg
    42121 ttgaccgcaa tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt
    42181 aagaccacat aaccaatcat tgccggatta aacgggtttt gccccagacc gccatacaac
    42241 tgtttagcga tgatcaccgc aaacaccgta cccagtacga ccatccacca tggcgctagg
    42301 gggggaatac ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt
    42361 gcggctaccg actgcttgcg tagtttgagt acgagagctt cggctaacag agcactgacc
    42421 gacgccaaca ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt
    42481 cctggcacgg ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg
    42541 gtataagggg agctagctat tctgaatacc atttagtcct cgtttacaac cttttgctgg
    42601 gcggcttttt tggcctgaac gcgggcaata gccgcggcaa ctgcggcttt gcgcggatcg
    42661 acctgttctt ctggttccgc attagcctgt tgctgttcca gcttgcgtgc tttggcgcgg
    42721 gcaatagcgg cttcgacggc agctttgcgc ggatcaacct gttcttctgg ctccgcattg
    42781 gcctgttgct gttccagctt gcgtgctttg gc
//
LOCUS       DAJTYG010000023        42761 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTYG010000023 DAJTYG010000000
VERSION     DAJTYG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.023"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..413
                     /locus_tag="OO568_001367"
     CDS             <1..413
                     /locus_tag="OO568_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6640784.1"
                     /translation="MYIGAPLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKL
                     FWRPKGLAYPLGEAPAKLRCTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFD
                     KYQFSLPKGAKNSSVTYRTINDYGAETPQLNCKS"
     gene            438..3037
                     /locus_tag="OO568_001368"
                     /pseudo
     CDS             438..3037
                     /locus_tag="OO568_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3028..4098
                     /locus_tag="OO568_001369"
     CDS             3028..4098
                     /locus_tag="OO568_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6640785.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESSDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4110..4652
                     /locus_tag="OO568_001370"
     CDS             4110..4652
                     /locus_tag="OO568_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6640786.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLIYQ"
     gene            4660..5175
                     /locus_tag="OO568_001371"
     CDS             4660..5175
                     /locus_tag="OO568_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6640787.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5141..5878
                     /locus_tag="OO568_001372"
     CDS             5141..5878
                     /locus_tag="OO568_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6640788.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRHEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5989..6999
                     /gene="pyrD"
                     /locus_tag="OO568_001373"
     CDS             5989..6999
                     /gene="pyrD"
                     /locus_tag="OO568_001373"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6640789.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7173..7715
                     /gene="zapC"
                     /locus_tag="OO568_001374"
     CDS             7173..7715
                     /gene="zapC"
                     /locus_tag="OO568_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6640790.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="OO568_001375"
     CDS             complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="OO568_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6640791.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            9065..11173
                     /gene="rlmKL"
                     /locus_tag="OO568_001376"
     CDS             9065..11173
                     /gene="rlmKL"
                     /locus_tag="OO568_001376"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6640792.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPSATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11185..13092
                     /locus_tag="OO568_001377"
                     /pseudo
     CDS             11185..13092
                     /locus_tag="OO568_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            13222..14475
                     /gene="pqiA"
                     /locus_tag="OO568_001378"
     CDS             13222..14475
                     /gene="pqiA"
                     /locus_tag="OO568_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6640793.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQLLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14480..16120
                     /gene="pqiB"
                     /locus_tag="OO568_001379"
     CDS             14480..16120
                     /gene="pqiB"
                     /locus_tag="OO568_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6640794.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16117..16680
                     /gene="pqiC"
                     /locus_tag="OO568_001380"
     CDS             16117..16680
                     /gene="pqiC"
                     /locus_tag="OO568_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6640795.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16936..17103
                     /gene="rmf"
                     /locus_tag="OO568_001381"
     CDS             16936..17103
                     /gene="rmf"
                     /locus_tag="OO568_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6640796.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="OO568_001382"
     CDS             complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="OO568_001382"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6640797.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17760..19520)
                     /locus_tag="OO568_001383"
     CDS             complement(17760..19520)
                     /locus_tag="OO568_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6640798.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPHWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19705..20157
                     /gene="matP"
                     /locus_tag="OO568_001384"
     CDS             19705..20157
                     /gene="matP"
                     /locus_tag="OO568_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6640799.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="OO568_001385"
     CDS             complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="OO568_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6640800.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="OO568_001386"
     CDS             complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="OO568_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6640801.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22363..22992
                     /gene="sxy"
                     /locus_tag="OO568_001387"
     CDS             22363..22992
                     /gene="sxy"
                     /locus_tag="OO568_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6640802.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="OO568_001388"
     CDS             complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="OO568_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6640803.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(25127..25573)
                     /locus_tag="OO568_001389"
     CDS             complement(25127..25573)
                     /locus_tag="OO568_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6640804.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25696..27750
                     /gene="helD"
                     /locus_tag="OO568_001390"
     CDS             25696..27750
                     /gene="helD"
                     /locus_tag="OO568_001390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6640805.1"
                     /translation="MELKATTLGKRLVQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="OO568_001391"
     CDS             complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="OO568_001391"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6640806.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28336..28998)
                     /locus_tag="OO568_001392"
     CDS             complement(28336..28998)
                     /locus_tag="OO568_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6640807.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29171..29584
                     /locus_tag="OO568_001393"
     CDS             29171..29584
                     /locus_tag="OO568_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6640808.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="OO568_001394"
     CDS             complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="OO568_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6640809.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="OO568_001395"
     CDS             complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="OO568_001395"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR6640810.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31289..31567
                     /gene="yccX"
                     /locus_tag="OO568_001396"
     CDS             31289..31567
                     /gene="yccX"
                     /locus_tag="OO568_001396"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6640811.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="OO568_001397"
     CDS             complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="OO568_001397"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6640812.1"
                     /translation="MLIFEGKEIEMDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="OO568_001398"
     CDS             complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="OO568_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6640813.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32850..32937)
                     /locus_tag="OO568_001399"
     tRNA            complement(32850..32937)
                     /locus_tag="OO568_001399"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32901..32903),aa:Ser,seq:tga)
     gene            33363..34481
                     /gene="hyaA"
                     /locus_tag="OO568_001400"
     CDS             33363..34481
                     /gene="hyaA"
                     /locus_tag="OO568_001400"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6640814.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLYYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPSCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34478..36236
                     /gene="hyaB"
                     /locus_tag="OO568_001401"
                     /pseudo
     CDS             34478..36236
                     /gene="hyaB"
                     /locus_tag="OO568_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            36255..36365
                     /locus_tag="OO568_001402"
                     /pseudo
     CDS             36255..36365
                     /locus_tag="OO568_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 b-type cytochrome subunit"
     gene            36400..36987
                     /locus_tag="OO568_001403"
     CDS             36400..36987
                     /locus_tag="OO568_001403"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6640815.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGRLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            36984..37382
                     /gene="hyaE"
                     /locus_tag="OO568_001404"
     CDS             36984..37382
                     /gene="hyaE"
                     /locus_tag="OO568_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6640816.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37379..38236
                     /locus_tag="OO568_001405"
     CDS             37379..38236
                     /locus_tag="OO568_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6640817.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINSTGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38370..39914
                     /gene="appC"
                     /locus_tag="OO568_001406"
     CDS             38370..39914
                     /gene="appC"
                     /locus_tag="OO568_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6640818.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            39926..41062
                     /gene="appB"
                     /locus_tag="OO568_001407"
     CDS             39926..41062
                     /gene="appB"
                     /locus_tag="OO568_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6640819.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41074..41166
                     /gene="cbdX"
                     /locus_tag="OO568_001408"
     CDS             41074..41166
                     /gene="cbdX"
                     /locus_tag="OO568_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6640820.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDLRLKS"
     gene            41246..42544
                     /gene="appA"
                     /locus_tag="OO568_001409"
     CDS             41246..42544
                     /gene="appA"
                     /locus_tag="OO568_001409"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6640821.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
BASE COUNT        10677 a        10405 c        11369 g        10310 t
ORIGIN      
        1 ttatgtacat tggtgcgccg ttggcgaaag acagagaaac ccttttcttc actagcgtac
       61 gggcagtccc ttcaacaacg aagcgggaag agggaaatac cctgaagatt gccacacaaa
      121 gcgtcatcaa acttttctgg cgaccaaaag gtttagcgta tcccttaggc gaggctccgg
      181 cgaaactgcg ttgcacttcg tcagctgaca tggttacggt cagtaaccca acaccttatt
      241 tcattaccct gacagacctg aaaataggtg gaaaagtagt taaaaatcaa atgatttccc
      301 cctttgataa ataccaattc tctctgccaa agggggccaa aaatagcagc gtaacgtatc
      361 gaaccatcaa tgactacggg gcggaaacgc cgcaactcaa ctgtaaatcg taagccgtct
      421 tcagttaaga gagcgagatg tatagaactc atcgacaaca cagcctgtta agctctggtg
      481 gagtgccatc gtttattggt gggctggtgg tgtttgtgtc ggcagcgttc aatgcacaag
      541 ctgaaacctg gttcgatcct gcttttttca aagatgatcc ctcaatggtg gccgatttgt
      601 ctcgtttcga aaaaggacaa aaaataacgc caggggttta tcgtgtcgat attgttctga
      661 atcagacaat tgtagatacg cgcaacgtca attttgttga gataacgcca gagaagggga
      721 ttgccgcctg tttgacgact gaaagcctgg atgcaatggg tgttaatact gatgcgtttc
      781 cggcttttaa acaactggac aaacaagcgt gtgtgccatt ggcggagatt attccggatg
      841 ccagcgtaac gtttaatgtg aataaactcc gtctggaaat ttcagtaccg caaatcgcca
      901 tcaaaagtaa tgcccgtggt tatgtccccc ctgaacgttg ggatgaaggg atcaacgcgc
      961 tattactggg atattcgttt agcggggcta acagtattca tagcagcgca gacagtgatt
     1021 ctggcgacag ctattttctg aatttaaaca gtggcgttaa tttaggccca tggagattgc
     1081 gcaacaattc aacatggagt cgcagtagtg gccaaaccgc agaatggaag aatctcagca
     1141 gctatttgca gcgggcggtt attccactaa aaggcgaact gaccgtaggt gatgattata
     1201 ctgcaggcga ttttttcgat agtgtcagct ttcgtggtgt gcagctggcg tcagatgaca
     1261 acatgctgcc agacagcctg aaagggtttg cgcctgtggt gcgtggtatc gccaaaagca
     1321 atgcccagat aacgattaag caaaatggtt acaccattta ccaaacttat gtatcgcctg
     1381 gtgcttttga aattagtgat ctctattcca cgtcgtcgag cggtgatttg ttagttgaaa
     1441 tcaaagaagc ggacggcagc gtcaatagct acagcgtacc gttttccagc gtgccattac
     1501 tccagcgtca ggggcgaatc aaatacgcgg tgacactggc gaaatacaga accaatagta
     1561 atgaacagca ggagagcaaa tttgcccagg ccacgttgca gtggggcgga ccgtggggaa
     1621 cgacatggta tggtggtgga caatatgctg aatattaccg tgccgccatg tttggtctgg
     1681 gatttaacct tggcgatttc ggagcaattt cgttcgatgc gacccaggcg aagagtacgc
     1741 tggcagagca aagcgaacat aaaggtcagt catatcgttt tctgtatgcc aaaacgctca
     1801 accaattggg cactaatttt caattgatgg gctatcgcta ttcgacgtcg ggtttctaca
     1861 ccctttccga caccatgtat aaacatatgg atggctacga atttaatgac ggtgatgatg
     1921 aagatacgcc gatgtggtcg cgttattaca atttgtttta caccaaacgt ggcaaactgc
     1981 aggtcaatat ctcccagcaa ttaggcgagt acggttcgtt ttatttaagt ggtagccagc
     2041 aaacttgctg gcataccgat caacaggatc ggctattaca gtttggctac aacacgcaaa
     2101 ttaaagatct ctcgctgggg gtttcctgga actacagtaa gtcccgtggt caacctgatg
     2161 ctgatcaggt gtttgcacta aatttttccc tgccgctcaa tctgttgctc cccagaagta
     2221 atgatagcta taccaggaaa aaaattacgc ctggatgacc tctaacacca gtatcgataa
     2281 cgaagggcac attacacaaa acctgggttt aacggagaca ctactcgatg acggtaacct
     2341 gagctacagc gtgcaacagg gatataacag cgaggggaaa acggctaatg gtagcgccag
     2401 catggactac aaaggggcgt ttgcagatgc ccgagtgggc tacaactaca gcgataacgg
     2461 cagtcaacaa caactgaact acgctctttc aggcagttta gttgcccatt cacagggcat
     2521 taccctgggg caatcgctgg gggaaactaa cgtgctgatt gcagcaccag gcgcagagaa
     2581 tactcgtgtg gcgaacagca ccgggctgaa aactgactgg cgcggatata ccgttgtccc
     2641 ctatgccact tcttatcggg aaaatcgaat cgcacttgat gcggcatcgt taaaacgtaa
     2701 cgtggatctt gaaaatgcag tagtcaacga ggttcccacc aaaggggcgt tggttctggc
     2761 ggagttcaat gcccatgcgg gagcaagggt attaatgaaa acatcaaagc agggtatacc
     2821 gctgcgtttt ggcgcgatag cgacgctgga cggcatacag actaatagcg gcattattga
     2881 tgatgatggc tcgctctata tgtccggttt gcccgctcag ggagcgataa ctgtacgctg
     2941 gggcgaagct cccgatcaaa tttgtcatat cagttaccag cttaccgaac aacaaattaa
     3001 ctctgcgatt acgcgaatgg atgccatatg cagataatct ttggagaaaa atgcgtgtta
     3061 ttactacgac tattttttgc cgccgtctta atgctatggt gcgctcaaac tgctgcttat
     3121 atcgggcagt gtcataccac tcaggggaat ccgtatattg gcgtcaattt tggcgttaaa
     3181 actctggatg aagaagaaaa tacggctgga gtagttaaag acaaatttta tcagtggaac
     3241 gaatcgagtg attattatgt ttcctgtgat tgcgataaag acaatgtcag aagtggccga
     3301 tgggcattcg ccgcggattc accgttagtc tatttaggcg acaactggta caaaattaat
     3361 gactatcttg ccgccaaagt tttattgcag gttaaaggca gttctcctac tgcggttcct
     3421 ttcgaaaacg tgggcacagg ggcagataca cgatggcata tttgcgatcc cggcggtcaa
     3481 cgtttaggtg gccagggggc tagcggtaat agcggtagct tttccctgaa aatattgcag
     3541 ccgttcgttg gttcggtcgt cattcctcct atggcgctgg cgcgattatt tgaatgctac
     3601 aacatacccg caggtgattc ctgcacgact actggtacac cggttttagt gtattacctg
     3661 tctggtacga tcaattcact tggctcatgt tccgtcaatg ccggagaaac aatcgaggtt
     3721 gatctgggcg acgtatttgc ggctaacttt cgtgttgtag ggcataagcc tcttggggcc
     3781 agaacggcag aacttgcaat tccggtcagg tgtaacacgg gaaacgcagg gttagttaac
     3841 gtcaatctga gtctgacggc aacctcagac cccagctatc cccaggcgat taagacgtca
     3901 cgtcctggcg tgggcgtggt ggtgaccgat agccagaaca acattatttc ccctgctggt
     3961 gggacactac cgctctctat tcctgatgat gcagacagta tcgcgcgaat gaatgtctat
     4021 ccagtcagca cgacaggcgt accaccagaa accgggcgat ttgaagccac ggcaacggtg
     4081 agaataaatt ttgattaacg cgtgaacgta tgaagaaaaa aacgatatat cagtgcgtta
     4141 ttttgttctt tagccttctt aacatccatg tcgggatggc tgggcctgaa caagtcagta
     4201 tgcatattta tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac
     4261 aaaatattca tattggtgat tttaatatca gtgattttca ggccgcgaat accgtaagca
     4321 ctgctgctga tttgaatatt gatatcaccg gttgtgccgc tggtattact ggcgcggacg
     4381 tcctttttag cggcgaggct gacacccttg cgccgacact gctcaaacta actgacacag
     4441 gcggaagcgg tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc
     4501 aagaaatccc gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac
     4561 tcaaatatca acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg
     4621 cggttctcta ttttgatctg atttaccagt gaaggagtga tgttgaaacg gataatctgg
     4681 attctgttct tactgggatt aacggggggc tgtgagctat ttgcccatga tggcacggtc
     4741 aacattagcg gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc
     4801 aatgtgcagt taggcgatgt ctcgctgaca cgtttttttc atgggaatta tggcccggag
     4861 aaatctttca tcattaattt gcaggattgc ggaacagatg tcagcacggt tgatgtcacc
     4921 ttttcaggaa cccccgacgg agtgcagagc gaaatgctgt ccattgaaag tggtacggat
     4981 gcagccagtg ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac
     5041 caggcgagta aagactacag cctacatagc ggtaaggtgc cattaacttt ttatgcgcaa
     5101 ttgcgacctg tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta
     5161 cttcattatg actaatacgt ggaacagatt agcgctcttg attttcgccg ttttatcgct
     5221 gctggtggcg ggtgaattgc aggcaggagt ggtggtcggc ggaacgcgat ttatctttcc
     5281 ggcagacaga gaatcgatat ctattttact gactaatacc agtcaggaat cttggcttat
     5341 taacagcaaa atcaatcgcc caacgcgttg ggccgggggg gaagcgtcga cagtgccagc
     5401 actattactg gccgctccgc cacttattct cctgaagccc ggtactacag gcacgttgcg
     5461 cttgctgaga acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag
     5521 cattgccagc gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc
     5581 ggtatttaaa ctgttctggc gacacgaagg tttgccgggc gacccgctgg aagcttatca
     5641 acaattacgc tggacacgga attcgcaggg tgtacaactc actaacccaa cgccttacta
     5701 cattaacctg attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagtcgtgcc
     5761 gcctaaaagc cagcgtcaga caaactggtg tcaggtaata gcaccgtgtc atgtcgcctg
     5821 gcggacgata aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa
     5881 caggttctga aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccggact
     5941 cgcacataat acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc
     6001 ttcgttcgta aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag
     6061 caattacgcc gcattaccgg aacaccgttt gaagcactgg tgcggcagaa agtgcctgcg
     6121 aaacctgtta actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt
     6181 gataaagacg gggagtgcat tgatgcgtta ggcgcgatgg gatttggatc gatcgagatc
     6241 ggtaccgtca cgccacgtcc acagcctggt aatgacaagc cgcgtctctt tcgtctggta
     6301 gatgccgaag gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta
     6361 gagaacgtaa aaaaggccca ttatgacggc gtcttgggta ttaatatcgg caaaaataaa
     6421 gatacgccag tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc
     6481 tatgcgggat atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg
     6541 caatatggtg aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg
     6601 caagcgatgc accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa
     6661 gacgaattga tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca
     6721 accaatacca ctctcgatcg ttctctggtt cagggaatga aaaattgcga tcaaaccggt
     6781 ggcttaagtg gtcgtccgct tcagttaaaa agcacagaaa ttattcgccg cttgtcacta
     6841 gaattaaacg gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg
     6901 cgtgaaaaga ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa
     6961 ggtccgccgc tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt
     7021 tataagataa tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt
     7081 atttagacat tttgtacttt tattgttgag gttattaagc gaagcaacaa tggattgtgt
     7141 tgctgcggtt ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc
     7201 gttggtatta cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat
     7261 ttcgctcacg ttttgcgcgc aaaatgttga ctccagatgc cttttctccc gcaggctttt
     7321 gcgttgatga tgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat
     7381 ctaaagacca aaaagccgaa ctggtgctga atgcactggt agcaattcgt tatctcaaac
     7441 cgcaaatgcc gaaaagctgg cattttgtct cccatggtga aatgtgggtg cccatgccgg
     7501 gcgatgcggc ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg
     7561 aatctggcga aaatgccgcg ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc
     7621 gcgcgatgca gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtga
     7681 atgttgacag cttcagcctc gaacaggcag tctaacgcgt cagcttaagg gcagtcttcg
     7741 gtacacagct acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg
     7801 tgacttcgcc ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat
     7861 aagggatacg aattccctga ttttctaatt gctccagcaa cacttgttgg ttatttccac
     7921 gaaatgcctg tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg
     7981 cggtatcatc agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca
     8041 cctcatcgcc aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga
     8101 catcgccgtt atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg
     8161 gatgtttttg ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa
     8221 ccacatcaaa caccacatca ccaatgcgaa tcactttcca gctatcttct tcccaggctg
     8281 acgcgccgga aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac
     8341 gttgttggag atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa
     8401 atgacagagg tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc
     8461 gtaattgcac ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa
     8521 ttcgcgcggt aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag
     8581 caaaacgaac atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag
     8641 gtgaaggagt aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag
     8701 gttccgtgat cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg
     8761 taagaccaat gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca
     8821 cggtgcggtc ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct
     8881 ataatgcgca acaattttct tagcgcctgt cccgttaggc tattttattt gtcagtttgg
     8941 ccctgggttg tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctctc
     9001 catgtccaga ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga
     9061 cgatatgaat tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga
     9121 actggaaaat ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg
     9181 cgacacacgg cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt
     9241 gccgctgggc gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat
     9301 caactggaca gagatgttta atcctagcgc gaccttcgct gtccacttca gtggtttgaa
     9361 tgacaccatc cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc
     9421 tttcacgcgg aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt
     9481 taacgtctgg ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt
     9541 acatctgcgt ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc
     9601 cgctattgtg atgcgatccg gctggcagtc aggaacaccg ctgctcgatc cgatgtgtgg
     9661 ttccggtacg ttgctgattg aagcggcgat gctggcgacc gatcgcgcac caggcttgca
     9721 ccgtgggcgt tggggcttta gcggctgggc gcagcatgat gaagctatct ggcaggaagt
     9781 gaaagcggaa gcgcaaactc gcgcccgtaa aggcctggct gagtatagct ctcattttta
     9841 cggttcggac agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg
     9901 gattggtgaa ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc
     9961 gaaagggccg tacggtacag tgttgagcaa cccgccatac ggtgaacgtc tggacagcga
    10021 accggcgctg attgcgctgc atagtctgct gggccgaatc atgaaaaacc agttcggtgg
    10081 ctggaatctc tctttgttta gtgcctcgcc ggatctgcta agctgcctgc agctgcgtgc
    10141 agacaaacag tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt
    10201 tgccgaaagc acaccagaca gtaaaccggc aatggtagcg gaagattacg ccaaccgcct
    10261 gcgtaagaac ctcaaaaaat tcgagaagtg ggcacgccag gaagggattg aatgttaccg
    10321 cctgtatgac gccgatctgc cggaatataa cgttgccgtt gaccgttatg ccgactgggt
    10381 ggtggtgcag gagtatgcgc cgccaaaaac tattgatgcg cacaaagcgc gtcagcgtct
    10441 gttcgatatt atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct
    10501 gaaaacccgt gaacgccaga agggcaaaaa ccagtaccag aaactgggcg agaagggcga
    10561 gtttcttgaa gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga
    10621 tactggtctg ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg
    10681 caaagatttc ctcaacctgt tttcttatac cggcagcgcc accgtgcatg cgggattagg
    10741 cggtgcacgc agcaccacca ccgtggatat gtcgcgtact tatctggaat gggcagaacg
    10801 caacctgcgt ctgaatggcc tgactaggcg tgcgcatcgc ctgattcagg ccgattgcct
    10861 ggcgtggctg cgtgaggcaa atgaacagtt cgatctgatc tttatcgatc cgccaacctt
    10921 ctctaactca aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc tggcgctgat
    10981 gaaagatttg aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg
    11041 tggcttccgt atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac
    11101 gcaaaaaacg ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat
    11161 taccgcagcc tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg
    11221 ttcagcgacg cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt
    11281 tgtctggtgg gccgcaacgg cgcaggaaaa tcgacgttaa tgaaaatcct caaccgtgaa
    11341 caagggctgg atgacggtcg cattatttat gagcaagatt tgattgtagc tcgtctgcaa
    11401 caggatccgc cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa
    11461 gaacaagcgg aatatctgaa acgctatcac gatatttcgt gcctggtgat gaacgacccg
    11521 agcgagaaaa atctcaacga actggcgaag gttcaggaac agctggatca ccacaacctg
    11581 tggcagctgg aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaacgtt
    11641 gcgctgtcgt cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg
    11701 agcaatccgc gcgtattgct gctggacgaa ccgactaacc atctggatat cgaaaccatc
    11761 gactggctgg aggggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac
    11821 cgttcgttta tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg
    11881 acctatccag ggaattacga ccagtacctg caggaaaaag aagaagccct gcgcgtggaa
    11941 gaactacaaa atgccgagtt cgatctcaaa ctggcgcagg aagaagtgtg gattcgctag
    12001 gggatcaaag cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcgatgcgt
    12061 cgcgaacgtg gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc
    12121 agccgctccg gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt taacggtaag
    12181 caactggtga aagatttttc tgcccaggtt ctacgtggcg acaaaattgc cctgattggt
    12241 ccgaatgggt gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaagcgggc
    12301 agcgggcgta ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg
    12361 gaactggatc ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg
    12421 gttaatggca agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa
    12481 cgggcgatga cgccagtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg
    12541 cgtttgttcc tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat
    12601 gtcgaaacgc tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg
    12661 gttagccacg atcgtcagtt tgtcgataac accgttacag aatgctggat cttcgaaggc
    12721 ggcggtaaaa ttggtcgtta tgttggcggt tatcatgatg cccgtggtca gcaagaacaa
    12781 tatgtggcac tcaaacagcc tgcggtgaaa aaaagcgaag aagccgccgc gccaaaagca
    12841 gaaactgtaa aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag
    12901 ctaccgcaat tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaagtggcg
    12961 gatgcttcct tcttcagtca gccgcatgag cagacgcaaa aagtgcttgc tgatatggct
    13021 gctgcagagc aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa
    13081 aatggtggct gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg
    13141 cctgatgaac tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata
    13201 gcgcatcatt aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc
    13261 tcgcagtgtg acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt
    13321 ccccggtgtg gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tcccaccgcc
    13381 tatgcgttgg ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg
    13441 aacgttgcag gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct
    13501 gaggactacg ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt
    13561 tgtctgataa ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag
    13621 caactggcac gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgcg
    13681 ggtgtgctgg tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc
    13741 tttctcccct ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt
    13801 cgctggttat gggacgacat cgccccgatg ccagaactgc gccagctgct aaaaccaggc
    13861 gtcacgggga tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct
    13921 gatgaacccg tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagcctg
    13981 cagtggacac tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg
    14041 cccatcatgg tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc
    14101 attctgttat ggagcgaagg ctcttatccc gtcgctgcgg tgatctttct ggccagtatt
    14161 atggtgccaa cgttaaagat gatcgccatc gcgtggctgt gttgggatgc caaagggcat
    14221 ggcaagcgcg acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc
    14281 tggtcgatga ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt
    14341 ttaatgagta tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca
    14401 atgttttctg ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag
    14461 catgaggagt cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa
    14521 gaactggtct cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct
    14581 tttttatcat tacagccatc agggaccgga agtgaccctg atcaccgcga atgcggaagg
    14641 aattgaaggt ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag
    14701 cgccacactg gctgatgatt tgacgcacgt tgaaatcaaa gcgcggctga attccggtat
    14761 ggaaaaattg ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga
    14821 agggattagc ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc
    14881 gaaaggcagc aaaatggata aatacgattt gctggactca ccaccgttgg ccccgcctga
    14941 tgcgaaaggt atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga
    15001 tccggtgctg ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca
    15061 aaaacgcaat atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag
    15121 caatgttcgc ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg
    15181 tgtggagatg ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga
    15241 aggtctggat ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca
    15301 gaagagcatt caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga
    15361 ttcggtacgc ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac
    15421 cgtaagcaaa gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg
    15481 tattccggta ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc
    15541 ggatgttgtt gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac
    15601 cggaaacctg gtcactggtg cactgtatgt tgatctcgat ttctatccaa atacgcctgc
    15661 aataaccggt attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggcct
    15721 ggcgcaaatc cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa
    15781 tccgatgatt gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct
    15841 gcaaacgacg ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc
    15901 gacggatatg cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg
    15961 ctccgcagcc tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga
    16021 actgcaaccg gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa
    16081 ggacaaaaaa gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt
    16141 gcagcactgt ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta
    16201 cctgtggtgc agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta
    16261 gagcaggtca ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat
    16321 gtgaagtatg tgattgccaa caacaacttg tgggccagcc cgttggatca acagttgcgc
    16381 aacaccctgg ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct
    16441 ctgggaagcg cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat
    16501 ggcaaggtca ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt
    16561 ccgttccgtc tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtgctg
    16621 gccggtgtct ggagtcagga agccgcttct attgcacaag agataaagcg tctaccttaa
    16681 ttataaagat ttgtaaatat aaccgtctcc ggtatgttgc ctgaggcggt ttttttgttt
    16741 ctaacgtgcg gaaaaatttg ttcctcttca cattttttgt acaaccgaca tgcccgtgta
    16801 gctcacaaat atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta
    16861 ttgcttaact gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta
    16921 tgagggaaac gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt
    16981 ggttatcagg ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat
    17041 caaaggtcac aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc
    17101 tgattctgtc tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct
    17161 gtctgatgat aatcagaagg cagacgtatc ctggaacaga ccgactttca ggtcgctggc
    17221 ggtatagatc agacgacaat caaccagcac ttcgccatct gccagaccca taatcagacg
    17281 acgattaaca atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac
    17341 ctgaccagtg aatttcactt cgccaacgcc cagcgcgcgg cctttacctt cgccgcccag
    17401 ccagccgagg tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat
    17461 aaccggatcg ccaatgaagt ggcatccgaa gaaccacaga tccggattga tatccagttc
    17521 tgcttcaaca taccctttgt cgaagttacc acccgtttcg gtcattttga ccacacggtc
    17581 catcatcagc atgttcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgtg
    17641 accagaggca agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt
    17701 aagccttatt ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat
    17761 cagttcggaa taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc
    17821 tgttgcgatg cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg
    17881 ccatcccaca ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag
    17941 atggtgaatt tgccttcttc taccgctttc accagttcac tgtgaagact taaatggcga
    18001 acgttagcag tggggataat gacaccttgt ttccccgtta actcacgttg ctggcaaata
    18061 gcaaagaagc cttcgatttt ctcatttaaa ccaccgaccg gctggacgcg gccgaactga
    18121 tcgactgaac ctgtgatagc gatactctga ttcactggca catcagcgag ggcgcttatc
    18181 agggcgcaga gttcagccat cgaggcacta tcgccatcca cttcactgta tgactgctca
    18241 aatgtcagcg atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac
    18301 gcctgcatga tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg
    18361 atgtcggtga attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca
    18421 aaagcgcgtg gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt
    18481 tcgccttcgg tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg
    18541 aggaaacctt cgcgccattc acgctgctgc agcattaagt ttagctgctc gccggagaag
    18601 gtgtcgccat cacacaggga ggcgacctct ttacactggc ggaggatcca atgcgggcta
    18661 agcggaagtg tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccaa
    18721 gcatccgctc ccggggcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac
    18781 cactggctta ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata
    18841 gcctgctctg aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct
    18901 accagaatga ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac
    18961 tcatcaaacg caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac
    19021 agcagaggtt gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga
    19081 tgcaccagac caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt
    19141 tgctccgctt ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct
    19201 gcactcactg cgtgacgtaa gcggatggag tggccggaaa cctcatagtg atcgcccacc
    19261 agttggcctg catcgctttg tagcgtacgc gcggcatcgg caataagatt cagatactca
    19321 gactcttccg gggccttagc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc
    19381 tccagcgcaa attgcagtcg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc
    19441 aaatgtggct gagcaaatat ttcctgatag ctatcggtat caggaaccaa gtcacgccat
    19501 gcaagtttcg taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata
    19561 ccgtaaccgg cactacagca cacgtagaaa gcaccgacaa tactcctggc tgggcgttaa
    19621 agctcacagg atggagattc ttttcttcac tggcctaaaa agctgatatt ctgtaaagag
    19681 ttacacggta acattgagat cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt
    19741 tggaaatgga agtatctggt gaagaagcat cgcgaagggg agttaattac ccgttacata
    19801 gaagccagtg ccgcccagga agccgtcgat gtgttgctct cgctcgaaaa tgaacccgtg
    19861 ctggtaaatg gctggattga caagcatatg aatccggaac tggtcaatcg catgaagcag
    19921 accattcggg ccagacggaa gcgccatttt aatgcggaac accagcatac gcgcaaaaaa
    19981 tcgatcgatc tggaatttat tgtctggcaa cgtctggctg gtcttgcaca gcgtcgcggt
    20041 aaaacgcttt ctgaaacgat tgttcagctg attgaagatg cggaaaacaa agagaaatac
    20101 gcgaataaaa tgtcttctct gaagcaggat ctgcaggcat tgctgggtaa ggaataactg
    20161 acgaaagtca gttcaattta ctaaaggcaa aaaaaacccc gcagcagcgg ggtttttcta
    20221 ccagacgata acttaagcct gcggctgagt tacaacgtct ttgatacctt taacttcgat
    20281 ctctacgcga cgatccggag ccaagcagtc gatcagtgca gcacgctgtt tcacgttgtc
    20341 acaggtgttg ccagtaaccg ggttggattc gcccatacca cgtgcggaga tcttgtctgc
    20401 cgggatacct ttggagatca ggtaatcaac aacagactga gcacggcgct cggacagacc
    20461 ctggttgtaa gcgtcagaac cgatgcggtc agtgtaaccc agaacaacta cggaaccgtc
    20521 tttcggatcc aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg
    20581 tttcagggtt gctttgttga agttgaacag aacgtcagac ttcagagtga agtgcttggt
    20641 ctgtacttcc ggtgccggag ctggagcaac taccggagct gcttcgccct gaccgaaacg
    20701 gtaggaaaca cccaggctca gcagaccgtt gtccggacga gtaccgatgg tgtttgcgtc
    20761 accgatgttg ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta
    20821 ctcaacacca cctgcgaaaa ccggagaaac gccagtgtcg tggtctttaa aggatgcgcc
    20881 accaggtacg ttagccttgg tgtctgcacg ccataccata ccacccagac gagtgtagat
    20941 gtccagatcg tcagtgattg ggtaacccag tttagcggtc agctgaacgc cctgagcttt
    21001 gtatgcgccg ttgatgttgt cgcctttgta cggcatacga cctaaccagt cgtaacccat
    21061 ttcaaaacca acatacgggt taacctggta accaccaaaa gcacctgcac ccagttggtt
    21121 ttcgtgggtc ggaccattgt taggaataaa accagtgtca tggtactggg accagcccag
    21181 tttagcacca gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc
    21241 agccagtgcc actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc
    21301 caaaatacgc catgaatatc tccaacgaga taacacggtt aaatccttca ccgggggatc
    21361 tgctcaatat taactctacc gatatcttcg gcttatgccg agcacccctg gcgatgtaaa
    21421 gtctacaacg tagttgaaaa cttacaagtg tgaactccgt caggcatatg aaaaaaaagt
    21481 cttgtataag gtatgtttaa tctttttgtc agcgacaatt tacagaaaaa aaacgcggaa
    21541 accgcttcag acaagccttc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg
    21601 acaaaaaaag ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt
    21661 ttagcccgga aagttgtctc gttgtgtgag aggatgcgct taccggacgc ataataaacc
    21721 ccatagcgtt accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat
    21781 catctgtcaa ccaaccgatc accacactgt aattgcccgt gcgtaaagcg cgaaccattg
    21841 actccactgt gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc
    21901 cagatgcctg aacccattcc cgactcagtt tttgttgcgg tgttaaccag agttgccagc
    21961 gcgattgctg accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct
    22021 gatcttcgcg atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac
    22081 gcgcaatttt acttgctgcg gatgagaacg acgaagaacg atgtgcatag cctgaagtgt
    22141 acataatcaa tccagcccct gtgagttact gtatggatgt gcagtacatc cagtgacaac
    22201 aaagatcaac cctattttcg gaaagagcct cgcaaatttt gtcgttggtg acgggaaaac
    22261 ataaattaat cttgcccctt aagaataagt tgcctatttt cgtagttaac ggatccgtta
    22321 atgtgaatca ttcttttatg ttatgatttt aaaaggaatt ttatgaaaag cctctcctat
    22381 aagcggatct ataaatcaca agaatacctg gcaacgttgg gcacaattga ataccgatca
    22441 ttgtttggca gttacagcct gaccgttgac gacacggtgt ttgcgatggt ttctgatggt
    22501 gagttgtatc ttcgggcttg tgagcaaagt gcacagtact gtgtaaaaca tccgcctgtc
    22561 tggctgacat ataaaaagtg tggccgatcc gttaccctca attactatcg ggttgatgaa
    22621 agtctatggc gaaatcaact gaagctggtg cgtctgtcga aatattctct cgatgcagcg
    22681 ctgaaagaga aaagcacgcg caatacccgg gaaagactga aagatttgcc caatatgtct
    22741 tttcatctgg aagcgattct cggggaggtg gggattaagg atgtacgggc gttacgtata
    22801 cttggggcaa aaatgtgttg gttgcgactg cggcagcaaa acagtctggt gacagaaaag
    22861 attctgttta tgcttgaagg tgccattatc ggcattcatg aagctgcgct cccggtggca
    22921 cgccgccagg agcttgcaga atgggctgac tctcttacgc cgaaacagga gtttcctgcg
    22981 gagcttgagt aatctggcgt tgcagacgac caatctctgg cagtaatgca atcaataacc
    23041 caacttgttg tacgaccaga ggttctttgc tatctgcccg tggttcaagt tgctgcatcc
    23101 gctgtttcag gccagctaat gcctgattga cgcgttcttc atcagcaggt tgatgatgta
    23161 acgcgtcatc aacatagcaa actgcgtcat caagaaacgc cagaatttca ggattagtta
    23221 actgctcccg gtgagcaccg agggctgaga tatagctggt aaacgtatgg ttaaggcaca
    23281 gcaagcgaaa cgcggcttcg cgaatttgcg gggtaacgtt cggttcgctg gacatatttg
    23341 ataccaccga cgccagttca gcatcacggt tgtgtgcatc gcggcgggca atacgatacg
    23401 ccagacggtt atcacgcccc tgatggtatt gctccagtat ggcatcgaga tagcgacagt
    23461 tggcctctgt ggcgcgttcg agcattcgcg gcagattgcg aaacttccag tcaggccaga
    23521 tgtagctcac tgccgcccac gcaatggcac aaccaatcag cgtatcgatt acgcgaggta
    23581 acgctacttc aaaaccttca cccagtaagt taaaacacag tagcaccaaa agtgtgatga
    23641 acatcgttgc atgagcgtat tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa
    23701 ccagcagcac cagctgccct tccagtgatg gcacaaacca cagcacagga atgccaatcg
    23761 cgatacctac cagcgtacca ataatcctta acttcaggcg gtggcgcgtg gcgttatagt
    23821 ttggctggca gacaaacaaa cttgtcagca agatccaata cccgtgatgc attccggtta
    23881 tttgaatgat ggcgtagccg aagcacaaca ccagcgacat tcttaccgca tgacggaaga
    23941 gggcggattc cggcgtgaag tgacggctaa gacgcagcca gatatcactc aatccgtgcg
    24001 ggctgtcatc agcgagctca ttttcgtcat tattacgggg tagtgcctgg gcctgttctg
    24061 attcaattgt tgccagttgg gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg
    24121 ttttgagtaa atcggcgggt gcgccgttat cgcgtatccg ctccagcgca gcatcaatat
    24181 gcgtaaaagc gcgctcaaaa tgcggatcat gttgataaga ctgacgcaac aaaatacagc
    24241 gtgacagttg ctggcacgcc tggccctgca tcgacatcag ccgctgaaaa cggaacagca
    24301 cgtcgctgtg gcgaaaatgt tcacgcaatg tttgatactg aatatgagaa gagctggcac
    24361 gctcgtgaat atcctgtgcg acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac
    24421 cacgatcgcc acgtaagcgg gtcagcagcg agagtttcgt ctgattcaat gtcgccatca
    24481 gctgaccgtt ggcgagagcc aaatcgtaca gcggtgcctg gctttcatct tcaatatcag
    24541 gatcaaacat gcgcgacttg agctcaagat aacgcgccag ttgttcatag caacgcgcca
    24601 ggttgtcctg cagcgggcgg accgggaaca gcagatgacc aataagtgtc aggacgttgt
    24661 accagacggc accggccagc agatacatcg gctgctgata ccagtgctca tacagtgatg
    24721 ttcccaacat agtgtaaatg gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt
    24781 gacccagacc gccgagcaaa atgaagccgc tggtagagag cgttaagcca atcgcaaata
    24841 gccagggcca gggaaacagc aattctactg aggccgaggc gataaaaaag cagaacagcg
    24901 taatgatgag gttacgcaaa cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg
    24961 ccaccatccc cagcgttagc ggaatcgtca gttttacatc acccagccac cacggaaacg
    25021 ctgtggttcc acaaagcgca ataaaaatac gcgcgtaata cagccaggcg ctgttccaag
    25081 tatagcgttt gagcaaagga cttagcataa aggccatatt agtggattat tcaaaacgac
    25141 gacgtgcatt ggcttcacgc gcagcttgcg ctgtttctac cgataccacg cgacgaccaa
    25201 ccggccatag tgcaatagcg gcaattttaa agttcgcaat gccgacagga atgccaatga
    25261 ttgaaataca ttgtgcgatg cccgttgcaa tgtgcatcag gcataaccac cagccaaaga
    25321 aaatcagcca gaaaatattc aataccgtac cgccagtatt cagcaacaca tttttgccag
    25381 ccgggttcag ttcatcgaca tgaatagctt cattgccata aggcaccaga gacagtttag
    25441 tgatctccca gcaggatcgt gtcagcggta aggtaaaaat cagcacaata ctgaccagag
    25501 tcgccaacag ccagcccaga gtggtggcaa atccgccaag cacaaagttc agaatgttca
    25561 aaacggtacg cataaaacct cgctttactg tggttttcag taatgggcgg caattgtaac
    25621 gtttttttgg gctggagcac gttttctctg acggttacac tgataagaaa taatttcgtg
    25681 tggatctaca gagtcatgga actgaaagcg acaacgcttg gaaaacgtct ggtacagcac
    25741 ccttacgatc gggcggtgat cctcaatgcc gggattaaag tctccggcga tcgccacgaa
    25801 taccttattc ctttcaatca attactggcg attcactgta agcgcggtct ggtatggggc
    25861 gagctggaat ttgtactgcc ggatgaaaaa gtggtgcgtc tgcacggcac agaatggggc
    25921 gagacgcagc gtttttacca tcatcttgat gctcactggc ggcggtggag tggcgagatg
    25981 agcgaaattg cgtctggtgt tttacgccag caactggatt tgattgccac gcgcactgga
    26041 gaaaataaat ggctgacgcg tgagcaaacc tctggcgttc agcaacaaat ccgccaggct
    26101 ttgtcggcgt tgccgttgcc ggttaaccga ctggaagaat tcgataactg ccgtgaggcg
    26161 tggcgtaaat gtcaggcttg gttgaaagat attgaaagcg ctcggttgca gcataaccag
    26221 gcgtataccg aagccatgct taccgagtat gcggattttt tccgccaggt cgagtcttca
    26281 ccgctgaatc cggcgcaggc ccgggcagtc gttaatggcg agcattctct gttagtgctg
    26341 gccggtgcag gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg
    26401 cgtggtgaag cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa
    26461 gagatggacg agcggattcg cgaacggcta cataccgaag acattaccgc acgcacgttt
    26521 catgcgctgg cgctgcatat tattcagcag ggcagcaaaa aagttccgat agtcagcaaa
    26581 ctggaaaatg ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc
    26641 agcgaaaaga aagcgcaggc gaagggctgg cggcaatggc tgacggaaga aatgcagtgg
    26701 tcagtgccag aaggtaactt ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc
    26761 ctcgatcgct gggtaagtct gatgcggatg cacggtggtg cacaggcaga aatgattgcc
    26821 agtgcacccg aagagattcg cgatctgttc agtaaacgta tcaagttgat ggccccgtta
    26881 ttaaaagcct ggaaaggtgc gctgaaggca gaaaacgctg tcgatttttc gggccttatt
    26941 catcaggcga ttgtgattct ggagaaaggc cgctttatca gcccgtggaa gcatattctg
    27001 gttgatgaat ttcaggatat ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag
    27061 caaaacagtc agacgacgtt gttcgccgtt ggtgatgact ggcaggcgat ttaccgattc
    27121 agcggtgcgc aaatgtcgct caccaccgct ttccatgaaa actttggtga aggcgatcgc
    27181 tgtgatttag acacgactta ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt
    27241 attcagcaga acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa
    27301 aaagctgtca cgttattgga tgagagtcaa cttgacgctt tgctggataa gctctctggt
    27361 tatgccaaac cggaagagcg cattctgatc ctggcgcgtt accatcacat gaggcctgcc
    27421 agcctggaaa aagcggcaac acgctggccg aagttgcaaa tcgactttat gaccattcat
    27481 gccagcaaag ggcaacaggc ggattacgtc atcatcgttg gcttgcagga ggggagtgat
    27541 ggttttccgg ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc accggttgag
    27601 gatttcccgg acgctgaaga acggcgttta atgtacgtgg cgctgacccg ggcacgccat
    27661 cgggtatggg cactgtttaa caaagagaat ccctctccct ttgtggaaat actgaaaaat
    27721 ctggatgtgc cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat
    27781 attacttcag acggtcagcg agataacgct gataatcggg gataagaata tcgaccgcgt
    27841 cgttgaaatg cggcgactgg ataatgaagt ctgccgttgc cacgttggtg gcgaccggaa
    27901 tgttccatac cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat
    27961 ttagtggatc ccagaagaaa atcaatacat caattttccc ttctgagatc aatgcgccaa
    28021 cctgctggtc accccccatt gggccactca acatcgcgtt gacgttcatg ccggtcgcgc
    28081 gggaaatcaa gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt
    28141 gatgccgttc cacccagctc atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa
    28201 tatgtttccg cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc cgtagttaac
    28261 tttcctacag attactgtaa gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc
    28321 acaggcacaa aaatctcagg aagatggttg tttttccgcc cattgcagga aagtatttcg
    28381 cgtttgtggg tcagccagtt taaaccaata cttcagccgt tgttctgtga gcacctgaga
    28441 ctgcggtgga atagcatcca gctcggaaac acccgaaagc agcgtactgt catctgccat
    28501 catcgtggca aattgcggta gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc
    28561 gacctcataa tcaatcgttt ttgccgttga ggtaatggcg agaatatcca gctttaccgg
    28621 aatcggcgtc gcatcgccat ccaacaattc aaggcgcggc gcggcatcaa aatggttagc
    28681 ttcccgctca ttctccaggc gaggcaggcg aaaattgacc tggttgatga gctgggtatt
    28741 aaaactgacc accagcggtg gggagatata cagccgttct tcactattgg aaagatgaat
    28801 cgtcttctca acgcgaaaca ctaactgatg cggtccattg tccagttcaa tgctgtccgc
    28861 accgcgcagc agagagctgg aaaccttttt gccgtcgagt accagaagat cgacatcggt
    28921 tgaaagccgc agcgtggtgg caaaaacgga taccggcaaa cataatgcaa tcaaggtggt
    28981 cacgatgccg gttttcatag gtagctcctg tcaaaagacc gcccgaataa taatgtgtca
    29041 aaatttttct ttaatcatac ttactaacat atagacatat ttcccgcttt tgctctcatt
    29101 cattcgtatt agctgcatgg ttggcatgtc ggctttgtcg tacactttgt taaacagtct
    29161 ggaggaaata atgaaagaaa ccgatattgc aggcatttta acgtccactc acaccattgc
    29221 gctggtgggg gcaagcgaca aacccgatcg ccccagctat cgcgtgatga aatatctact
    29281 cgaccagggg tatcacgtca ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg
    29341 ccagcagggg tatggcacgc tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt
    29401 ccgtaattca gaagcggcgt ggggcgtggc acaggaagct attgccattg gcgcgaaaac
    29461 cctctggatg caactggggg tgattaatga gcaagcggca gtactggcac gggatgccgg
    29521 gttaaatgtg gtgatggatc gctgcccggc tattgagatc cctcgcctgg gtctggccaa
    29581 ataaaaaatc cccggaaggc aaaaaccttc cggggatttg ttcagggatt agttacgcag
    29641 acgcggggcc tggagttgtt tgcggatggt ctgcgccagc tcatccatgg atggctgttc
    29701 aggatgttca tcctgcaatt cgctacttag ctgggcttca gccaggtagg tatgaaccgg
    29761 taggccgtta tcgtcctcca tcaccacgtg ataccacgga gcggcgcgaa gctcgtcatt
    29821 caccgccagc tcatcaggcg acggttcaga gagcgaataa accgggtcga tatccacgac
    29881 cactccgaga taacctaaca gggaatggcg gacctgctgg ccgataccga atttgctggc
    29941 aatcatagtc acctcccggg aaatctttac ctttgatata agggtaaaat tccacatttc
    30001 aagttacatg acgcgacagg caaacccttt cagatatagc ccttccgggt aggtagcgat
    30061 caccggatga tcggctgcct gacggaactg ctctataaat tgtacatcac gaccggcatc
    30121 aattgcggca tccgcgatga ttttctgaaa taaatcgctg gtcatcagac cggaacagga
    30181 gaaagtcagg agaataccgc cttcattcag caactgaatc gccagcatgt tgatgtcttt
    30241 ataaccacga cacgcgccca tcaactggct tttattctca acaaacttcg gcgggtccat
    30301 cacgataacg tcaaattttt caccgcgatc gcgataagta cgcagcaatt taaagacatc
    30361 atcacggaca aactcagcct tgctcagatc cagtttgttc agctcaacgt tctgccgtgc
    30421 aatatccagc gcttcctggg aggtatcaac gctgacaacc tggctgcaac cgcccatcag
    30481 tgccgatacg gcgaaaccac cggtatagga gaaacagttc agcacacgtt tattttcaac
    30541 gtagcggcgg gtagccaggc ggctgtctcg ctggtccaga tagtagcccg ttttgtgtcc
    30601 gtgctgaata tccaccagca gcttcatgcc gtgttcttca atcggcagca gggcaggcgg
    30661 caactcgccg gtgacgaggc cctgggtcag ctccatccct tcttttttac gtaccgcaac
    30721 atcgctgcga tcgtaaatcg cacattccgg gtacagcgtt tgcagggcac taattaatgc
    30781 cgcgcgctga tattctgcgc cagcactcag cagttgcagc accagaaaat taccgaaacg
    30841 atcgatagta atacccggca ggccatcaga ttctccggcg attaaacgat agctgtcgag
    30901 gccatctttt tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa
    30961 aaaagcaatg tcgatagact cagacgggtc aaacgtccag acgcgcgccc ggatttgcga
    31021 agctggcgaa taagcgccgc gtgctaacca ttttccctga tgatcaacaa tatcgatggt
    31081 ttcaccgagg ctggctttac cttccatgcg ggcaacggcc ccggaaaaga cccacggatg
    31141 gcgacgaagt aatgattttt cgcgcccttt ggctaacact aaacgtacac tcataattta
    31201 cttttctgtc gatgcaaaga aatgggcgtc attgtccgga gttttccatg gatttgcaac
    31261 gcgtccatta aggataagga gagcagcaat gtcgaaagtc tgcataattg cctggattta
    31321 cgggcgagtt cagggcgtag gatttcgcta caccacacag tacgaagcga aaagactggg
    31381 cttaaccggg tacgccaaaa atcttgacga tggcagcgtg gaagtggttg cctgcggtga
    31441 agaagggcag gtagaaaaat taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg
    31501 tgtagaacgg gtgctcagcg agccgcatca tccctcgggg gaattaaccg attttcgtat
    31561 ccgttaaata cattttaccg gttttggcag gccagcaatt ttggtggctt gcttagccgg
    31621 gcctttcggg aacagtcggt ataaatagcg gctgttacct ttttcttcac caaatttatt
    31681 cgccatcgct tttaccagca tacgaatcgc cggagacgta ttaaattcca ggtagaagtc
    31741 acgtacaaaa cgcaccactt cccagtgttc tggcgacagc gaaatccctt cgttctctgc
    31801 aatcaccact gccagcggtt cactccactg gctgctttct ttgagatagc cttcggtatc
    31861 catttctatc tctttacctt cgaagatcag cataattatt actaccttaa tcagactggc
    31921 ggcagtgtaa caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg
    31981 agattaatcg cggctggcga agcccagaat gctcagcagg ctgacgaaga tgttgtacag
    32041 cgaaacatac aggctaaccg tggcacgaat atagttcgtt tcaccgccat gaatgatgtt
    32101 gctggtttca aacaagatag cgccagagga gatcagaatg aagaccgcgc tgatcgccag
    32161 atgcagagca ggcagttgca ggaagatatt agcaaccata ccaatcagta ccaccacaat
    32221 acccgccatc agcataccgc cgaggaacga catatctttg cgggtggtca gcacatatgc
    32281 agagcagcag aagaacacta acgccgttcc gcccagtgcc atagcgatta cgtcacccat
    32341 tccggcagac agataggtgt tcagaattgg tccgaggata taacccagaa aaccggtaaa
    32401 ggcgaatgcg gagataatcc cggtcggctt attcgccgtt ttataggtca ggaacatcaa
    32461 accatacata cccaccagcg tcagaatcag acccggagat ggcagcatca gcaccgtact
    32521 ggcagttgcg gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat aggtattacg
    32581 cagcacctta tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa caatacgatc
    32641 cattgagtca ctctctatga cagatgtaat taattaagca gcataatgat aatgcgtaag
    32701 ggcacccaga agttttaccc atctttacgc atttgatctg gaacaggttt aacagcggat
    32761 tatcaggtca ttaagcaaat ataacgccct gagaattccg acaggcaaaa gaaaaagggg
    32821 ttagcattta gctaacccct tatcttattt ggcggaagcg cagagattcg aactctggaa
    32881 ccctttcggg tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga
    32941 atgacgcgca ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct
    33001 gaaaaacagc caaaagtgca ttgatagcgt gaaataacag cagattgatc atttcatcac
    33061 catgaattcc ttctctttta ctcgtttagc aaccggctaa acatccccat cgcccggcta
    33121 aagaaaaata ggtccatttt tatcgcaaaa agataaatcc acacagtttg tgttgttttg
    33181 tgcaaaagtt tcactacgct ttattaacaa tactttctgg cgacgtgtgc cagtgcagaa
    33241 ggatgagctt tcgttttcag catctcacgt gaagcgatgg tttgccttgc tacagggacg
    33301 tcgcttgccg accataagcg cccggtgtcc tgccggtgtt gctaggagaa gagacgtgcg
    33361 atatgaataa cgaggaaaca ttttaccagg ccatgcggcg tcagggcgtt acccggcgca
    33421 gctttctcaa atattgtagt ctggctgcca cgtcgctggg attaggcgcg ggaatggcac
    33481 caaagattgc ctgggcgctg gagaacaaac cgcgcattcc ggtggtatgg atccacggtc
    33541 tggaatgtac ctgctgtacc gaatctttta tccgctccgc tcacccactg gcgaaggacg
    33601 tcatcctttc cctgatttcc ctctattacg acgataccct gatggctgcc gccggaaccc
    33661 aggcggaaga agtctttgaa gacatcatca cgcaatataa tggcaaatat atcctcgcag
    33721 tagaaggtaa tccgccgctg ggcgagcagg ggatgttctg tatcagcagc ggtcgaccgt
    33781 ttattgagaa actcaaacgt gccgctgccg gagccagcgc gattatcgcc tggggaacct
    33841 gcgcgtcctg gggctgcgtg caggccgcgc gacctaatcc gacacaggca acgcctatcg
    33901 acaaagtcat caccgacaaa cccattatca aagtacctag ctgcccgccg atcccggatg
    33961 tgatgagcgc catcattact tacatggtga cctttgatcg cttgccagat gtcgacagaa
    34021 tgggtcgtcc actgatgttt tatggtcagc gtatccacga taaatgctat cgccgcgctc
    34081 acttcgacgc cggagagttc gtccagagtt gggatgatga cgctgcccgc aaaggttatt
    34141 gcctgtacaa aatgggctgc aaagggccta ccacctataa cgcctgttcc tccacacgct
    34201 ggaatgatgg cgtttctttc ccaatccagt ctggtcacgg ctgcctgggc tgtgcggaaa
    34261 atggtttctg ggatcgcggt tcgttctaca gccgcgtggt cgatattccg caaatgggta
    34321 ctcattccac cgccgatacc gtcggtttaa ccgcgcttgg cgtggtggca gcagctgttg
    34381 gtgtgcacgc agttgccagc gccgttgacc aacgcagacg tcataaccag caacctacag
    34441 aaaccgaaca tcagccaggc aatgaggata aacaggcatg agcactcagt acgaaactca
    34501 gggatacacc atcaataatg ccggacgccg cctggtggtc gacccgatta cgcgcatcga
    34561 aggccacatg cgctgcgaag tgaatattaa cgatcagaat gtgatcacca atgccgtctc
    34621 ctgcggcacc atgtttcgcg ggctggagat catcctgcaa gggcgcgacc cgcgcgatgc
    34681 gtgggcgttc gttgaacgta tctgcggcgt ctgtactggc gtacacgccc tggcttcggt
    34741 ttacgccatc gaagatgcca tcggtattaa agtgccggac aacgccaata tcatccgcaa
    34801 cattatgctg acaacgctct ggtgccacga tcatctggtg cacttctatc agcttgccgg
    34861 gatggactgg atcgacgtgt tagatgcgct gaaagccgac ccgcgaaaaa cctccgaact
    34921 ggcgcaaagt ctctcctctt ggccgaaatc atcccctagc tatttcttcg acgtacaaaa
    34981 tcgcctgaag aaattcgttg aaggcgggca gttggggatc ttccgcaatg gctactgggg
    35041 gcacccgcag tacaaattgc cgccagaagc caacctgatg ggctttgccc actatctcga
    35101 agctctcgat ttccaacgtg aaattgtcaa aattcacgcg gtctttggcg gtaaaaaccc
    35161 gcatccaaac tggattgtcg gcgggatgcc ttgcgccatt aacattgacg aaagcggcgc
    35221 ggtcggggcg gtcaatatgg aacgcctgaa cctggtgcag tcgattatca cccgcacggc
    35281 agacttcatt aacaacgtga tgatccccga cgccttagcc atcggtcagt tcaacaaacc
    35341 gtggagcgaa atcggcacgg gtctttctga taaatgtgtc ctcagctacg gcgcattccc
    35401 ggatattgcc aacgactttg gagagaaaag tctgctgatg cctggcggcg cggtgattaa
    35461 cggcgacttc aacaatgtgc tgccagtgga tttggttgat ccgcagcagg tgcaggagtt
    35521 tgtcgatcac gcctggtatc gttatcccaa cgatcaggtc gggcgtcatc cattcgatgg
    35581 cattaccgac ccgtggtaca accccggcga tgtcaaaggc agcgatacca acattcagca
    35641 gctgaatgaa caggaacgct actcgtggat caaagcgcca cgctggcgcg gtaacgcgat
    35701 ggaagtgggg ccgctggcac gcacgttaat cgcttatcac aaaggcgatg ctgcgaccgt
    35761 tgagtcggtc gatcgcatga tgtcggcgtt gaacctgccg ctttccggta tccagtcaac
    35821 gttgggccgc aggtaagttg cagtatttct tcgacaagct gatgaccaac ctgaaaaatg
    35881 gcaatctcgc cactgcttcc actgaaaaat gggaacctgc aacctggccg acagagtgcc
    35941 gtggcgtcgg ctttaccgaa gcgccgcgcg gagcgttagg ccactgggcc gccattcgcg
    36001 atggcaagat tgatctctac cagtgcgtgg tgccgaccac ctggaacgcc agcccgcgcg
    36061 atcccaaagg gcagattggc gcttatgaag cggcgctgat gaacaccaaa atggcgatcc
    36121 ccgagcaacc gctggagatc ctgcgtactc tgcacagctt tgatccgtgc ctcgcctgtt
    36181 caactcatgt gctgggcgac gacggtagcg agctgatctc cgtgcaggtg cgttaacagc
    36241 gaaggagaat catcatgcaa cagaaaagcg acaacgttgt cagccactat gtctttgaag
    36301 caccagttcg tatctggcac tggctgacgg tgttatgcat ggcggtgttg atggtcaccg
    36361 gttacacaaa tttggcaaaa taagtaacaa ggagcgttca tgagcgagca acgcgtggtg
    36421 gtcatggggc tgggcaacct gctgtgggcc gatgaaggct tcggcgtgcg ggtggcggaa
    36481 cggctgtatg cccattacca ctggcccgag tatgtggaga ttgtcgatgg cggtactcag
    36541 ggactgaact tgctggggta tgtcgaaagc gccagccatc tgttgattct cgatgccatt
    36601 gactacgggc tggaacctgg aaggcttcga acctatgccg gagaacgcat tccggcctat
    36661 ctcagcgcga agaaaatgag cctgcatcag aacagtttct ccgaagtgct ggcgctggca
    36721 gatatccgcg gacatctgcc agcacatatt gccctcgtcg ggctgcaacc cgcgatgctc
    36781 gacgactacg gcggcagcct gagcgaactg gcacgggagc aactgcccgc tgcggaacaa
    36841 tcggcgctgg cgcagttggc tgcgtgggga agtgtgccgc aaccggctaa tgaatcgcgt
    36901 tgtctcaatt atgactgtct gtcgatggaa aattacgaag gcgttcgctt gcgccagtac
    36961 cggatgacac aggaggagca gggatgagca acgacacgcc atttgatgcg ttgtggcaac
    37021 gaatgctggc gcgcggctgg acgccggtca gtgaatcccg tcttgacgac tggcttacgc
    37081 aagcgccaga cggcgtggtg ttattaagca gtgacccgaa acgcacgcca gaggtcagcg
    37141 ataatccggt aatgattggc gaattactgc acgagtttcc cgactataca tggcaggtgg
    37201 cgattgctga ccttgagcag agcgaagcca tcggcgatcg ctttggcgcc tttcgctttc
    37261 ctgccacttt agtgtttacc ggcggaaact atcgcggcgt gctgaatggt attcacccgt
    37321 gggcggaact gataaacctg atgcgcgggc ttgtcgaacc gcagcaggag cgtgcctcat
    37381 gagcgaaact tttttccatc tgctggggcc aggaacgcaa ccgaacgatg acagtttcag
    37441 catgaatcca ctgccgatca cctgtcaggt gaatgatgaa ccgagtatgg cggccttgga
    37501 gcaatgtgct cacagcccgc aggtgattgc gctgttaaac gagttacaac atcaactaag
    37561 cgaacgccag ccgccgttgg gcgaggtgct ggcagtcgat ctgttaaatc tcaacgccga
    37621 cgatcgtcac tttatcaata cgcttctcgg ggaaggggaa gtgtcagtgc gcattcagca
    37681 ggctgacgac agtaaaagtg aaatacagga ggcgatcttc tgcggattat ggcgggtgcg
    37741 cagacgtcgc ggcgaaaagt tgctggagga caaactggag gctggctgcg cgccgctggc
    37801 gttgtggcag gcggcaacgc aaaatctctt gccgacagat tcgctgttac cgccgcccat
    37861 tgatggcctg atgaatggcc taccgttggc gcatgagtta ctggcacatg tacgtaaccc
    37921 cgacgcgcag ccgcacagca ttaatctgac gcaattaccc atcagcgagg ctgatcggct
    37981 ttttctctca cgtctctgtg ggccgggaaa tattcagatt cgtaccattg gctatggcga
    38041 gagctatatc aactccacgg ggttacgcca tgtctggcat ttacgctgta cggacacctt
    38101 aaaaggcccg ttactggaaa gttatgaaat ctgcccaata ccggaagtgg tgctggcagc
    38161 gccagaagat ttggtcgact ctgcgcagcg gcttagcgag gtatgtcagt ggctggcgga
    38221 agctgcaccg acgtaaaaag acggtaagta tcgctttcag tcttatgaat atcgcaatcg
    38281 gcgaatacct ctggtcgtag agtttcagga taaagaggga gatctaacat tatcgggtta
    38341 tttttctctc ttcgcctaca ggagtgcgca tgtgggatgt cattgattta tcgcgctggc
    38401 agtttgctct gaccgcgctg tatcactttt tatttgtacc ccttaccctg gggctgattt
    38461 ttttgctggc tatcatggaa accatttacg tggtcaccgg caaaacaatc taccgcgata
    38521 tgacgcgctt ctggggtaag ctcttcggta tcaattttgc tcttggcgtg gctaccggcc
    38581 tgaccatgga gtttcagttt ggtactaact ggtcattcta ttccaactat gtgggcgata
    38641 tttttggcgc accactggcg atggaagcat taatggcctt cttcctcgaa tccacctttg
    38701 tcgggctgtt cttcttcggc tggcaacggc tgaataaata ccagcacctg ctggtgacgt
    38761 ggctggtggc gttcggttca aatctctctg cattgtggat attgaatgcc aacggttgga
    38821 tgcaataccc gaccggtgcg cattttgata tcgacaccct gcgtatggag atgaccagct
    38881 tcagcgagct ggtctttaac ccggtcagcc aggtgaaatt tgtgcacacc gtcatggcgg
    38941 gctacgtgac cggggccatg tttattatgg cgatcagcgc ctggtattta ctgcgcggac
    39001 gggagcgcga tgtcgcatta cgctcgtttg ccatcggttc cgtcttcggt actctggcga
    39061 ttatcggtac cctgcaactc ggagacagtt ctgcgtatga agtcgcgcaa gtacaaccgg
    39121 taaaactggc ggcgatggaa ggggagtggc aaacggaacc tgctcctgca ccgttccatg
    39181 tggttgcctg gccggaacag gatcaagagc gtaacgcctt tgccatcaaa attcccgcgc
    39241 tgctagggat cctcgccact cactcattag ataaacccgt gccgggtctg aagaatttga
    39301 tggctgaaac ctacccacgc ttgcaacgcg gacgtatggc ctggctgtta atgcaggaaa
    39361 tatctcaagg caatcgtgag ccgcatgtgt tgcaggcatt ccgggagctg gaaggcgacc
    39421 ttggttacgg catgttgctc tcccgctatg cgccggatat gaatcatgtc acagccgcac
    39481 agtaccaggc ggcgatgcgt ggcgcgatac ctcaggttgc gccggtattc tggagtttcc
    39541 gcatcatggt gggctgtggt tccctgctgc tactggtgat gctgattgcg cttgtccaga
    39601 cgctgcgtgg caaaatcgac cagcatcgct gggtgctgaa aatggcgctc tggagtttgc
    39661 cgttgccgtg gattgcgatt gaagccgggt ggtttatgac cgagtttggt cgtcagccgt
    39721 gggcgataca ggacatctta ccgacatact ccgcgcactc cgctttaacc acaggacaac
    39781 tggctttctc actgatcatg atcgtaggtc tttacaccct gttcttaatc gccgaagtct
    39841 acctgatgca gaaatatgcc cgtctggggc cgagcgcgat gcagagtgaa caaccgacgc
    39901 agcaacaggg gtaaaggaga aaatcatgtt tgattatgaa acattgcgct tcatctggtg
    39961 gctgctgatt ggcgtgatcc tggtggtctt tatgatctcc gacggatttg acatggggat
    40021 cggctgtttg ctgccgctgg tggcgcgtaa tgatgatgaa cgccggatag tgattaatag
    40081 cgttggtgcg cactgggaag gcaatcaggt ctggttgatc ctcgcaggtg gggcattatt
    40141 tgccgcctgg cccagagtgt atgcagcggc gttttccggc ttttatgtgg cgatgatcct
    40201 ggtgctgtgc tcgctgttct tccgtccgct ggcctttgat tatcgcggaa aaattgccga
    40261 tgcacgctgg cgtaaaatgt gggatgccgg tctggtcatc ggcagtctgg tgccgccggt
    40321 agtcttcggt atcgccttcg gcaacttgtt gctcggcgtg ccgtttgcct tcacaccgca
    40381 attacgcgtg gagtatctcg gcagcttctg gcaactgctg acgccattcc ctttattgtg
    40441 tggtttgctc agccttggga tggtgatttt gcaaggtggc gtctggttac aactgaaaac
    40501 tgttggtgtg attcatctgc gttcacagct ggcgactaaa cgcgctgcac tgttggtgat
    40561 gctgtgcttt ttgctggcag gttactggct gtgggtcggt attgatggct ttgtactgct
    40621 cgcccaggat gctaacggtc cttccaatcc gttaatgaaa ctggtggcag tgctacctgg
    40681 tgcctggatg aataatttcg tcgaatcgcc cgttttgtgg atcttcccgc tgctggggtt
    40741 cttctgccca ttgctgacgg tgatggcgat ttatcgtggt cgcccgggtt ggggattttt
    40801 gatggcatca ttgatgcaat ttggcgtgat tttcacggca ggcatcacgc tgttcccctt
    40861 tgtcatgccg tcaagtgtga gtccaatctc cagcctgacg ttgtgggaca gcacttccag
    40921 tcagctgacg ctgagcatta tgttggtaat cgtgctgata tttttgccca ttgtgttgct
    40981 ctacactctc tggagctact acaaaatgtg gggacgcatg acaacagaaa ctctccgccg
    41041 taacgaaaac gagttgtact aaggagcagg aacatgtggt atttactttg gttcgtcggc
    41101 attttgttga tgtgttcgct ctccaccctt gtgttggtat ggctggacct gcgtctgaaa
    41161 agttaacgaa cgtaggcctg atgcggcgca ttagcatcgc atcaggcaat caataatgtt
    41221 agatatgaaa agcggaaaca tatcgatgaa agcgatctta atcccatttt tatctcttct
    41281 gattccgtta accccgcaat ctgcattcgc tcagagtgag ccggagctga agctggaaag
    41341 tatggtgatt gtcagtcgtc atggtgtgcg tgctccaacc aaggccacgc aactgatgca
    41401 ggatgtcacc ccagacgcat ggccaacttg gccggtaaaa ctgggttggc tgacaccgcg
    41461 cggtggtgag ctaatcgcct atctcggaca ttaccaacgc cagcgtctgg tagccgacgg
    41521 attgctggcg aaaaagggct gcccgcagtc tgctcaggtc gcgattattg ctgatgtcga
    41581 cgagcgtacc cgtaaaacag gcgaagcctt cgccgccggg ctggcacctg attgtgcaat
    41641 aaccgtacat acccaggcag atacgtccag tcccgatccg ttatttaatc ctctaaaaac
    41701 tggcgtttgc caactggata acgcgaacgt gactgacgcg atcctttgca gggcaggagg
    41761 gtcaattgct gactttaccg ggcatcggca aacggcgttt cgcgaactgg aacgggtgct
    41821 taatttcccg caatcaaact tgtgccttaa ccgtgagaaa caggacgaaa gctgttcatt
    41881 aacgcaggca ttaccatcgg aactcaaggt gagcgccgac aatgtctcat taaccggtgc
    41941 ggtaagcctc gcatcaatgc tgacggagat atttctcctg caacaggcac aaggaatgcc
    42001 ggagccgggg tggggaagga tcaccgattc acaccagtgg aacaccttgc taagtttgca
    42061 taacgcgcaa ttttatttac tacaacgcac gccggaggtt gcccgcagtc gcgccacccc
    42121 gttattggat ttgatcatgg cagcgttgac gccccatcca ccgcaaaaac aggcgtatgg
    42181 tgtgacatta cccacttcag tgctgtttat tgccggacac gatactaatc tggcaaatct
    42241 cggcggcgca ctggagctca actggacgct tccaggtcag ccggataaca cgccgccagg
    42301 tggtgaactg gtgtttgaac gctggcgtcg gctaagcgat aacagccagt ggattcaggt
    42361 ttcgctggtc ttccagactt tacagcagat gcgtgataaa acgccgctgt cattaaatac
    42421 gccgcccgga gaggtgaaac tgaccctggc aggatgtgaa gagcgaaatg cgcagggcat
    42481 gtgttcgttg gcaggtttta cgcaaatcgt gaatgaagca cgcataccgg cgtgcagttt
    42541 gtaatgcata aaaaagagca ttcagttacc tgaatgctct gaggctgatg acaaacgaag
    42601 aactgtctaa tgcgtagacc ggaaaaggcg ttcacgccgc atccggtcac tttcagtttt
    42661 actctttctc ggagtaacta taaccgtaat agttatagcc gtaactgtaa gcggtgctgg
    42721 cgcgtttaat cacaccattg aggattgcgc ctttaatatt a
//
LOCUS       DAJTYG010000024        40250 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTYG010000024 DAJTYG010000000
VERSION     DAJTYG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.024"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..789)
                     /locus_tag="OO568_001410"
     CDS             complement(<1..789)
                     /locus_tag="OO568_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR6640822.1"
                     /translation="MTQYSSLLRALAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQVLKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLG"
     gene            1036..1662
                     /gene="gstB"
                     /locus_tag="OO568_001411"
                     /pseudo
     CDS             1036..1662
                     /gene="gstB"
                     /locus_tag="OO568_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(1659..2773)
                     /gene="yliI"
                     /locus_tag="OO568_001412"
                     /pseudo
     CDS             complement(1659..2773)
                     /gene="yliI"
                     /locus_tag="OO568_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2884..3267)
                     /gene="bssR"
                     /locus_tag="OO568_001413"
     CDS             complement(2884..3267)
                     /gene="bssR"
                     /locus_tag="OO568_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6640823.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3480..4805
                     /gene="rimO"
                     /locus_tag="OO568_001414"
     CDS             3480..4805
                     /gene="rimO"
                     /locus_tag="OO568_001414"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6640824.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNIKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4852..6180)
                     /locus_tag="OO568_001415"
     CDS             complement(4852..6180)
                     /locus_tag="OO568_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6640825.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVESINLSTSIGVAHSNKCTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6188..8535)
                     /locus_tag="OO568_001416"
                     /pseudo
     CDS             complement(6188..8535)
                     /locus_tag="OO568_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8713..9624)
                     /gene="gsiD"
                     /locus_tag="OO568_001417"
     CDS             complement(8713..9624)
                     /gene="gsiD"
                     /locus_tag="OO568_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6640826.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9627..10547)
                     /gene="gsiC"
                     /locus_tag="OO568_001418"
     CDS             complement(9627..10547)
                     /gene="gsiC"
                     /locus_tag="OO568_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6640827.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10565..12103)
                     /gene="gsiB"
                     /locus_tag="OO568_001419"
     CDS             complement(10565..12103)
                     /gene="gsiB"
                     /locus_tag="OO568_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6640828.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDSGFSFED
                     ADLQ"
     gene            complement(12123..13994)
                     /gene="gsiA"
                     /locus_tag="OO568_001420"
     CDS             complement(12123..13994)
                     /gene="gsiA"
                     /locus_tag="OO568_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6640829.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(13981..14946)
                     /gene="iaaA"
                     /locus_tag="OO568_001421"
     CDS             complement(13981..14946)
                     /gene="iaaA"
                     /locus_tag="OO568_001421"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6640830.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMESVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15150..16385
                     /gene="moeA"
                     /locus_tag="OO568_001422"
     CDS             15150..16385
                     /gene="moeA"
                     /locus_tag="OO568_001422"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6640831.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16385..17134
                     /gene="moeB"
                     /locus_tag="OO568_001423"
     CDS             16385..17134
                     /gene="moeB"
                     /locus_tag="OO568_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6640832.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17313..17975)
                     /gene="fsa"
                     /locus_tag="OO568_001424"
     CDS             complement(17313..17975)
                     /gene="fsa"
                     /locus_tag="OO568_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6640833.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            18106..19005
                     /locus_tag="OO568_001425"
     CDS             18106..19005
                     /locus_tag="OO568_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6640834.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19011..21443
                     /locus_tag="OO568_001426"
     CDS             19011..21443
                     /locus_tag="OO568_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6640835.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHYELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21589..22404
                     /gene="ybiV"
                     /locus_tag="OO568_001427"
     CDS             21589..22404
                     /gene="ybiV"
                     /locus_tag="OO568_001427"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6640836.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22555..23820
                     /locus_tag="OO568_001428"
     CDS             22555..23820
                     /locus_tag="OO568_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6640837.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24061..25653)
                     /locus_tag="OO568_001429"
     CDS             complement(24061..25653)
                     /locus_tag="OO568_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6640838.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEGFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            25872..26792
                     /gene="ldtB"
                     /locus_tag="OO568_001430"
     CDS             25872..26792
                     /gene="ldtB"
                     /locus_tag="OO568_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6640839.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(26850..27968)
                     /locus_tag="OO568_001431"
     CDS             complement(26850..27968)
                     /locus_tag="OO568_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6640840.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFQTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     SQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGMQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(27965..28432)
                     /gene="mntR"
                     /locus_tag="OO568_001432"
     CDS             complement(27965..28432)
                     /gene="mntR"
                     /locus_tag="OO568_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6640841.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28618..28746
                     /gene="mntS"
                     /locus_tag="OO568_001433"
     CDS             28618..28746
                     /gene="mntS"
                     /locus_tag="OO568_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6640842.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29018..30601
                     /gene="opgE"
                     /locus_tag="OO568_001434"
     CDS             29018..30601
                     /gene="opgE"
                     /locus_tag="OO568_001434"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6640843.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYKTFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30649..31164)
                     /gene="ompX"
                     /locus_tag="OO568_001435"
     CDS             complement(30649..31164)
                     /gene="ompX"
                     /locus_tag="OO568_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6640844.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGLNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31217..31282)
                     /locus_tag="OO568_001436"
     CDS             complement(31217..31282)
                     /locus_tag="OO568_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640845.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31517..32404
                     /gene="rhtA"
                     /locus_tag="OO568_001437"
     CDS             31517..32404
                     /gene="rhtA"
                     /locus_tag="OO568_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6640846.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32703..33206
                     /gene="dps"
                     /locus_tag="OO568_001438"
                     /gene_synonym="pexB"
     CDS             32703..33206
                     /gene="dps"
                     /locus_tag="OO568_001438"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6640847.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33610..34356
                     /gene="glnH"
                     /locus_tag="OO568_001439"
     CDS             33610..34356
                     /gene="glnH"
                     /locus_tag="OO568_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6640848.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEVYKKWFSTEPK"
     gene            34495..35154
                     /gene="glnP"
                     /locus_tag="OO568_001440"
     CDS             34495..35154
                     /gene="glnP"
                     /locus_tag="OO568_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6640849.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35151..35873
                     /gene="glnQ"
                     /locus_tag="OO568_001441"
     CDS             35151..35873
                     /gene="glnQ"
                     /locus_tag="OO568_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6640850.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36134..38358
                     /gene="ybiO"
                     /locus_tag="OO568_001442"
                     /pseudo
     CDS             36134..38358
                     /gene="ybiO"
                     /locus_tag="OO568_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38355..39281)
                     /gene="rlmF"
                     /locus_tag="OO568_001443"
     CDS             complement(38355..39281)
                     /gene="rlmF"
                     /locus_tag="OO568_001443"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6640851.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39557..39817
                     /locus_tag="OO568_001444"
     CDS             39557..39817
                     /locus_tag="OO568_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6640852.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            40082..>40250
                     /locus_tag="OO568_001445"
     CDS             40082..>40250
                     /locus_tag="OO568_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640853.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAP"
BASE COUNT        10009 a        10636 c         9796 g         9809 t
ORIGIN      
        1 ccccagcact acggagatta aacgcatatc accctgggta gccgaagcaa ccagattata
       61 tcccgcacct gccgtggttc ctgtcttcat gccatcaaca ttcagattgc tgctccacag
      121 cagacggtta cggttaggct gacgaatttt gttgaaggtg aactctttct ctttatgaat
      181 ggcgtactct tccggcacat cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc
      241 ggtactgaac tgccccggcg catccaggcc gtgcaccgtc tggaaggtag tgttggtcag
      301 ccccagtttt ttggcataac cattcatcag accaataaag gactcctggc tcccggcaac
      361 gtaatcggcc agcgcaatac aggcgtcatt accggactgg ataatcacac ctttgttcaa
      421 gtctgccacc gaaacctgat cgcccggttt gaggaacatt accgatgaac cacgcagtgc
      481 cggatttccc gtcgcccagg catctttacc gaccgtcacc atatcggtga gtttaatctt
      541 atcggcctta agcacctgcc caaccacata gctggtcatg attttagtca ggctcgcggg
      601 atccagtttc tcatccgcat tgccttctgc cagcacttta ccgctggcgt aatccattaa
      661 aatccatgca cgcgcatcca cgctcggcgc ctcaacggtt tgttcggccg cgaatgccgt
      721 tggggcaaaa aggaataaaa atgcagaacc cgctgcaaga gcacgaagga gagaggagta
      781 ttgcgtcata aatgccaccc gagtatccat tcaaaaaaaa tacgttatat cgccgtatga
      841 taagttcggt gattaataac gcactcacaa acttaaagaa accgtaagtt ggtaaagttt
      901 ttaaagttta tgcaatatcc tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg
      961 ttgcttaaaa gttaacttta cttcaccatc ggagtcctgt tcagtctgtg gctgcctgtc
     1021 ggaaaagggg tgagtatgat tacgctgtgg ggtcggaata attcaactaa cgtaaaaaaa
     1081 gtattgctga cgctcgaata actggaacta ccttatgagc aaattctcgc gggccgtgag
     1141 tttgggataa atcacgatgc tgattttctg gcgatgaacc ctaacgggct ggtgccgttg
     1201 ttacgtgacg acgaaagtga tctcattctt tgggaatcaa acgccattgt ccgctatctg
     1261 gcggcacagt acgggcaaaa acgcctgtgg atcgactcac cggcacgtcg tgcggaagcg
     1321 gaaaaatgga tggactgggc aaaccagacg ctcagtaatg ctcatcgcgg gatcctgatg
     1381 ggattagtca gaacaccacc ggaagagcgc gatcaggccg ccattgatgc cagttgcaaa
     1441 gagtgcgacg ccctgtttgc cctgctcgat gcggaactgg caaaagtaaa atggttctct
     1501 ggcgacgagt ttggtgtggg cgatatcgct atcgcaccgt tcatctacaa tttgtttaac
     1561 gttggcctga cctggacacc gcgtccaaat ctgcaacgct ggtatcagca actcactgaa
     1621 cgccccgcag tgcgcaaagt ggtgatgatt cccgttagct aattgcgtgg gctaacttta
     1681 agtaattccc cactggactc gtcggtgaga acgtataaat aaccgtcggg tccagtgcga
     1741 acatcacgaa ttcgctgccc tctgtccgtt aaaatacggc catcttctgt cactttgtcg
     1801 ccgttgacgc tcatcacaat gacatcttta tctttcagcg cccaataaat aatttttgct
     1861 gccactgggg gaatttatcg ctgttataga aggccatgcc gctcacagcg ggcgaatctt
     1921 tccagtaaaa aacaggttgc tcggtcccgg cgacgatctc ccctttcgct tccggtatct
     1981 taaagcctga atagttgatt ccccaggttg ccagcggcca gccgtagttt ttgccttttt
     2041 gcgggatatt aatttcatca ccaccgcgcg ggccatgttc attcagccac agtgcattac
     2101 tccacggatt catcgccatt ccttgcggat tacgaatgcc ataagaccag atctcggcgc
     2161 gcgcaccgga ttcctttata aaaggattat catccgggat ttcgccctgg tcggtcagac
     2221 gcaccagttt gccctgtaat ttatccagat cctgcgccgt cgggcgctga ttgttttcgc
     2281 ccagagcaat aaaaagataa cctttaccgt cgaataccag ccgcccgcca aaatggttgc
     2341 cggtagacag ttttggcatc tggcggaaga cggtgcggaa gtcggtcact tttgagagat
     2401 catcacttaa gcggccataa cccacagcag ttccggcttt gccatcatcg ccaacttcgg
     2461 aataacttaa ccagatgcgg cgagactgag caaaatcagg cgctaaaacc acgtccagca
     2521 ggccgccctg cccgtgcgcc caaacgtccg gaactccgga aagcggcgca gataatcctt
     2581 ttcctgcttg ccagtgacgc aactcgccgc cgcgcagagt gattaacata ccgtgattat
     2641 cgggtaaaaa ggccagtgcc cagggatggt cgagtttgtc ttgcagtact tcgacattta
     2701 ccgttgcagg agccgcccag agagcggaag aaagacaaat aaggggcaca aggaaaaagg
     2761 attgtcgatg cataacgcct ccggattgac tctggcttaa agcgtagtca gtggaggaga
     2821 taacaaattc atttttacaa aaacttaaac atgaaggggg agacgctttc tcccccttag
     2881 ttttcaggcc ttctcaagca tggcgtgctt ctgcaggctc tggatactca gcgttaagct
     2941 catcagacaa ttttcaagct tatcggcgtt gacggtaata acagtcgggc aatcatggtg
     3001 cccactcatc aaacatactg cggctgtcgc taatgcttct tcagcatgat gaagagcact
     3061 ccactcttcc tgatccagat gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt
     3121 cagtttaaaa aagttatctc gtagatgatt gctttcgctg acggacatgt atccttttgc
     3181 cttcctcagt tgcacgtatg cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag
     3241 atcgattcgc tgtctgtcaa cgaacatact tcgatcctcc tctttccagc cttgttgcaa
     3301 ttaaatagta tggctatttt ttgagcaaaa tgctgtctag gatcaattct tcacgccgaa
     3361 ttaacaaaat ttacatttcc ttcgcctccg ccagcgaatg agattgcgat acaaatttta
     3421 tccagaactc tgtagaatcc ctgccctgac ctttcctgta actgaatatt ttttaagcta
     3481 tgagcaaagt aactccccag ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc
     3541 ttgtcgattc agagcgtatt ctcaccgaac tccgcactga aggttatgac gtggtaccga
     3601 gctatgacga tgcggacatg gtgatcgtca acacctgcgg ctttattgac agcgcggtac
     3661 aagaatcact ggaagccatt ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg
     3721 gttgtctggg ggcaaaagaa gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca
     3781 ccgggcctca tagctatgag caggttctgg agcacgttca tcactacgtg ccaaaaccga
     3841 aacacaaccc attcctgagc ctggtgccag aacaaggtgt gaaactgacg ccgcgtcatt
     3901 atgcctatct gaaaatttcg gaaggctgta atcaccgctg caccttctgc attattccgt
     3961 ctatgcgcgg cgacctggtg agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc
     4021 tggtagatgc gggcgttaaa gagattctgg tgatctcgca ggatacttcc gcctatggcg
     4081 ttgatgttaa acatcgtact ggcttccaca acggcgagcc ggtaaaaacc agcatggtca
     4141 gcctgtgcga acagttatcg aaactgggga tctggacacg tctgcactac gtttaccctt
     4201 atccgcatgt ggacgacgtc atcccactga tggcagaagg caaaatcctg ccgtatctgg
     4261 acattccgtt gcagcacgcc agcccgcgca ttctcaaact gatgaagcgt ccgggttctg
     4321 tagatcgcca actggcgcgc atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac
     4381 gctcaacctt tattgtcggc ttccctggcg agacggaaga agatttccag atgctactcg
     4441 acttcctgaa agaagcacgt ctggatcgcg ttggctgctt taaatacagc ccggttgaag
     4501 gtgcagacgc caatgccctg cctgaccagg ttccggaaga agtgaaagaa gaacgctgga
     4561 accgtttcat gcagctgcag cagcagattt ccgccgagcg cctgcaagag aaagtgggcc
     4621 gtgaaattct ggtgattatc gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg
     4681 cagatgcacc ggaaatcgac ggcgcggttt atctcaatgg tgaaaccaac attaagccgg
     4741 gtgatatcct gcgtgtgaaa gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg
     4801 tttaattaat tcaggccggg caaaatcatt tgtccggcct gcgaataatg attactgcca
     4861 gctaatctgt gccctgccgt ttttcttact cttatacagc cgctcatccg ccagcatttt
     4921 caagcgttcg acggtagtac atttatttga atgcgctaca ccgatactgg tgcttaaatt
     4981 aatgctttcc acctggaaat gaacgatttc ttttagtatc gatagcaact cttccagcgt
     5041 gccgccagta taaatcaccg caaattcgtc acctccgaag cggtagactt tacctttatc
     5101 taccgggatc tgtgatttaa ggattttcac cactttcatc aacacttcat cgcccagcaa
     5161 atgaccgaag gtgtcgttaa tgtttttgaa tttatcgacg tcaaacagac acaaataacc
     5221 gcctttttcc ggcagcgctt ttaaatcttc atcaaaggat tttctgccca ttgctttggt
     5281 caggtcgtcc aggaacgccg agttatacat actgtagagt tttttgacat cggcagccga
     5341 aaagaaaagc ccgccatcca gtacgcgttc aaaatggtta ttaatgatgg cttctttctc
     5401 cacaatacca cgattaatca ctctgcgtac cagatagagg caactggcac ccgatatcac
     5461 tgccaccaat gcggcaaaga aaataatcaa tgtacaaacc cgcaaataaa catctttttt
     5521 atacgcaaat acgtacaatc cggtcggcaa ataggcgaag tgctccatcg cttccgaaac
     5581 ggtgaagatg gcgtttttag cattatattc atcaccggtc accggtttac cgttcttaaa
     5641 tagagagaac atcaggttct cgctgtccat cgccagcgat tttgacagta aagaaaaacg
     5701 ctccagacca agcaaaaata tcagactacg attgtgagtt accggataga ggtaatagac
     5761 gaaggtttta ccatcaaaag agataaaccc tgtatttttg acatgtcgat acttcacaac
     5821 ctgcataata tctgcacaat aatttttctc gaactccttt acctgaatac attcgtcact
     5881 ggaaatcaga taaggaccga catcgtacaa ataagagggg gaaaccgagt cggcgtaagc
     5941 tgtgcgcaaa ccaaccaaca gctgttcctc cgtgggattt agcgtgttga taagatcaat
     6001 tgccacgcta cgccgatatt cagcaatatt tgccaccgca aaagcagtat caataacgga
     6061 ctgtttcagg ctatacaccc gttcctttac catttgagtg tagatcccgc aggcaactgc
     6121 tgaaatcatg ataaaaatca gcagactgac tgtaagtacg aacttattga ttctggacat
     6181 acgtaaacta ctcttttact aattttccac ttttatccca ggcggagaat ggcatgggtt
     6241 tctggtacag atacccctga gcctgaacac atcctaattt aattaattcg cgcgcaacgt
     6301 cttcgctctc tacgccttcc gcaatcaccg ggatccggtt atagcgtgcc agatcgaaaa
     6361 taatgcgcac aatttttccc gcagtaccga aaatatcgcc ggtaaattgc gagtccacct
     6421 ttatgctgtc tggattaatt tcataaaaac gtttcaggtt tgacaaaccg gtgccaaagt
     6481 catcaatcga tatccgtacc ccttgtttac gcagttgttc aatattgaga taaaaatgtt
     6541 cattaattcg ctcgatcacg gtttcagtga tctcaacaca gaggctgttc gccttcaggc
     6601 cattttcttt catgatggtg gtaaaacgct catgaaagtg cggttcgttt aactgccagg
     6661 gcgaaacatt aatatgcagt aagaaatcat ccttcagcga cgcacgttga ctaaaatgtc
     6721 tgaattcacc cattgccgtt ttaataatct gataacccag ctcattgata aacccgatat
     6781 cttcagcaat ggtaatgaac ttcagcggtg aaatgatccc ccgctccgca gaaacccaac
     6841 ggcacaacgc ctccgcgctc agaatggcgc gggtgttaat atcaacgatc ggttgataga
     6901 cgaggaatag ttcgccaatt tccattgcat ggcgaatatt acgcgcaatc cagatattct
     6961 cttctatttc gttgagtttc gcgtcatcac agaaaataaa ctcggagaac atatggtcac
     7021 ggatactgga aatcgccagt ttcagttttt ctgcgtaact ccaggcgttt ccccctgcaa
     7081 aggttcacaa atggcagact tatggatgcg cagattgcgg tagccaaagc tgctgtcaaa
     7141 atcgtttacc atcgctattt tacgggtaaa tgtctgtaca ttttcccgac acagaagtaa
     7201 atagagatcg gcattgaatg tcacgatatc gcagtattgc gcatatttct cggcaattcg
     7261 ctgacacaat ttcctgacga ttttattgcg ttcatcaaca caaaacaccg cttccacggc
     7321 ttccagacca cacagtttga tgatccccac agccagatac tgattattgt tggactcaat
     7381 aatcgccata tttttaataa aaccgctttc ctgatcgtaa gagatcagtg agagcaattt
     7441 atttgaccgt tcaatttcgg catctttcat ttccgcgatc gaatttttga tgctgataat
     7501 ctctttcgca tcgccttcgc gaattttcag agcctcgatc tgatccgttg agtcgggcaa
     7561 aaacgccagc gtattaacca gctcggtcat ttcagaaaat tgccgacgga acacacgagt
     7621 ggtcatgaaa tataccacca gcaacacgat aataatgaac gcaaacggta gataaaaaag
     7681 tttatctgat gcgttcaata aattaccgcc aggataataa aaatagattt cgaacaatgc
     7741 attatttggc actgctgatg tctttgcaat atactccgta tcgacagaat caataatatt
     7801 ataattacct tctctattat aaataatcag tttttcacga gcgagttgat tcttggcaat
     7861 gatctcatga ccttgtttca aaaatagaac ttcatcatta gcgctgattt gatcaagata
     7921 ctcgttcaga taccgccagt ttaaatcgac atgaatttta tctaccgcta acaaatcgtg
     7981 atctttgccg ttgatacgta gcgtaaaaga gatacatttt ttacccggac gatcaaagca
     8041 attgtatgcc ggattccagc tgttgttaga gtcatctgaa gccaccgacc acggtcgttt
     8101 cgatgggtta aaatcggcaa aaataacccg ccctgactcg tcgacaagcg gtccatttac
     8161 ttgttcctga tgataaacaa cgattttatc attcgccctc cgcgaaaaga gataatattt
     8221 attatcatca agtaccagtc ccaccgagtc taaaaatggc atcatttcca ttttcagaat
     8281 cacggcgcgt tgaatctcct ctgtattttt taataaatac agtgagtcct ttaatgtcag
     8341 ggccgaacgt tcaatatctt taaagaaata gccaacgttt ttctgaataa gttcaaagcg
     8401 gctatcggca cgttttaccg cgcgttccga taccagttga tagttaatca tgaaaaaaag
     8461 accaataaat gacagtgctg ccaataaaaa taaaattatc agccttatct ttatcttttc
     8521 gtataaactc agcatgttaa tacatttaac aaaataacta tctgaacgct gtgccgctgc
     8581 aaaaatagtc ccttgctgcg gctttttaac caaattaccc gattctacaa aatcagcaga
     8641 aagttacaac acttgcttag ggcaataatt tcctgtgagg tgattaccct ttcaagcaat
     8701 attcaaacgt aactatcctt taattttcgg atccagcgca tcgcgtaaac catcgcccaa
     8761 caaattgaac gccagtacgg tcagaaaaat agccagggcc ggaaaaacag cgacatgcgg
     8821 cgcgataacc atatccgctc gtgcctcatt gagcattgct ccccactctg gtgtcggcgg
     8881 ctgcgcacct aggccgagaa atgacaggct ggcggcagag ataatcgagg tgccaatgcg
     8941 catggtgaaa aacaccacga tagaagagac ggtcccaggc aggatatgac gcaataaaat
     9001 ggtcatatcg ctggcaccaa tactgcgtgc tgactcaata aaggtttgct gtttcaacac
     9061 cagcgtgttg ccgcgcacca ggcgggcaaa cgcggggatg gaaaaaatgg cgactgcgat
     9121 aatcacgtta gcaatgccgc ttcccaacac cgcaacaaca gcgatcgcca gtaaaatacc
     9181 cgggaaggca aacagcacat cgcaaatgcg catgatcagc cgatcccacc agccttcata
     9241 atatccagcc aacaagccca gcaacgtccc tatcgccaca ccgataaaca cggcaaacac
     9301 gcccgccgcc agcgagattt gcgcaccaac caggacacgg ctgaaaatgt cgcgccccag
     9361 tgaatcgacg ccaaaccagt gctgcaaaga aggtccgtta ttcagattgt cataatcaaa
     9421 ataattttcg gcgtcatagg gagcgatcca gcgtgcaaaa atggccacca caatcaataa
     9481 aataacgaat aatgcggcgg tcatcgccat atgctgacgg cgaaatcgtc gccagaattc
     9541 atgccacggt gtacgtacct ggtcaggttt gaccagtggc atggcgttta acaccgcctg
     9601 acgtcgccag ttaaatagtc gcatccttac ttgtacctga tagccgggtt aatggcggcg
     9661 taaagcacat ccaccactaa gttgataaga ataaattcca gcgagaaaag cagaatttcc
     9721 gcctgaatca ccgggtaatc gcgcatttct acagagtcaa cgagtaagcg tccaagtccg
     9781 ggccagttga aaactttctc caccacgata gaacctccga gcaaaaagcc aaactgtaag
     9841 cccatcatgg tcactaccgg gatcatcgcg ttgcgtagcc cgtgtttgag gacaacccag
     9901 gtttcgctca cccctttcgc cctcgcggta cgcatataat cttcgcttaa aacgtcgaca
     9961 aatgacgcac gggtaaagcg cgccatcacg gcggccactg ccgcgccgag cgtcagggag
    10021 ggtaaaatgt agtgctgcca actgtctgct cccacagtag gcagccagcc cagttcaacg
    10081 gagaatacct gaattaaaaa catccccagc gcaaatgccg gaaacgagat ccccgacacc
    10141 gcaatggtca tactcaatcg atccggccaa cgattacgcc agacggcggc gataattccc
    10201 gccgccatac caaatatcac cgcccagacc atactggtta tggtcagcca cagcgttggc
    10261 ataaagcggc tggcaatctc atcggcaacc ggacgacgcg acaccatcga caggccaaaa
    10321 tccccctgca cagcattgct gatatagtgc cagaactggt gatacagcgg ctgatccaac
    10381 cccagctgct gacgcaccag ttctataacc tgcgcatcag cttcgggccc ggcaatcaat
    10441 cgcgccggat cgccgggcag catgtggaca aataaaaaca ccagcaccga gacgataaac
    10501 agcgtcggaa tcaaccccag taatcgtttg ataacgtaat taagcattcc actccctgcg
    10561 ttgcttattg caaatccgcg tcttcaaagc tgaagccgct gtctggcatg atccaaaaac
    10621 cggtcaggtt tttactgtgt gccgacacca gtttttctac caccagcggg atccacggcg
    10681 attcttgcca gatgatatcc tgcgccgcct tatataagcg ggtcttttcc gccggatcat
    10741 tcgttttcag tgcctgagcc aggaagtcat ccacctgttt attgctgtaa aacgcggtat
    10801 taaacagcgt cggtggccag ttttgcgagg caaacagcgg cgatagtgcc cagtcggctt
    10861 cgccggttga agccgaccag ccagtgtaga acatccgcac gccgctctct ttttgccctt
    10921 taccttcaac ttctgccgcc cgctgtccgg catccatcgc ggtcacctgg gctttaatcc
    10981 cgacctgcgc taactgctgc tgggtaaatt gcagcacttt ctgcgcggtg ctgtggttat
    11041 gtgacgacca cagcgtggta ctgaaaccgt tgggatatcc cgcctctttc agtaattcgc
    11101 gcgctttcac tggatcgtaa ggccacggtt tataactttg cgcgtaggcg atacttggcg
    11161 gtaccacacc agtagctggc gttgcatagc ccgcaaaggc aactttcacc agcgccggac
    11221 ggttaatggc gtaattcagc gcctcacgga ccttcgggtt atcgaacggc ttttgcgtca
    11281 cgttcatact gatataacgc tgcataattg acggactggc catcaactcg atatttttgt
    11341 ttttctccag cagtgtggcc tgctcgtagg gaatggggaa agcaaactgc gcttcaccgg
    11401 tttgcagcat tgccgcgcgg gtgttgttat ccgccaccgg acgccaggtt atgctgtcca
    11461 gtttgggcaa tcctggctgc cagtaacccg cgaatttttt caccttcaca aaatcggtct
    11521 gattccaggt atccagttca tacggtccgg ttcccaccgg atgaaaacca atctccttgc
    11581 catatttttc cagcgctgtc ggtgaaatca tcgcggtcgc cggatgggca agaatattaa
    11641 taaacgctga gaacggctgt ttgagggtaa tctttaccgt tgtcggatcg atcgcctccg
    11701 ttttggcaat attcttatac aggttatagc gtttaagatg attcgccgga tcgctggccc
    11761 ggtccagatt cgcttttacc gccactgcgt tgaaatcggt gccatcctgg aatttaattc
    11821 cttcccgcaa tttcacggtg taagtaatgc cgtcatcgga aacggtataa ctctccgcca
    11881 gcacgttttt cagtttcatc tctttatcca gaccgaacag cccctggtaa aacgatttcg
    11941 ctacggcctg agataacgtg tcattcgcgt catacggatc gagcgtggtg aaattcgatc
    12001 ctaccgccac caccacatct ttggcagcga atgcacaaga tgccatcaac gctgtcgcaa
    12061 tgcccagcgc cactaaccca ctacggtgta cagctcttgc cattttattc tccgcctgaa
    12121 tgttatctac gcatgaatgc gtattctgat tgtggttgtg cgacgtaatg ccccggcccg
    12181 acgcattgca acgagacggc tgccacctct tcgccacgca gatgaatatt gctgggaaga
    12241 tcgtccgaca gcagtacacg ctgcggtcgt tgtcgggacg gttcagcgac cggaactgcc
    12301 gccagtaatt tacgcgtata aggatgctgc gggttttcga agaccgcgca ccgtgaacca
    12361 atttcaacaa tttgcccgag atacatcacc gccacacgat gactaatccg ctctaccaca
    12421 gccatatcgt gggagataaa cagatacgca atgccgaaat cacgctggag atcgagcaac
    12481 aagttgataa tctgcccgcg aatagaaaca tccagcgccg aaacggcttc gtcggcaatg
    12541 atcacttttg gattcaatgc caacgcgcga gcaatgcaga tgcgctggcg ctgaccgccg
    12601 gaaaactcat gcgggtaacg ccaggcatgt tcaggtaaca ggcccacgcg ctccagcaac
    12661 cacgcaacgc gtgcagccgc gtctttacct ggcaataaac cgtgtacacg cagcggttcg
    12721 ataatcgaat caccgatggt ctgacgtggg tccagcgaag cgtaagggtc ctgaaaaata
    12781 aactgaatat cccggcgcaa tgcctgaagt ttgccaggtg acaaggtatc gattcgctga
    12841 ccgttaaaga taatttcgcc gccctgcgat tcgaccaggc gcagcaacgc ccgcccggta
    12901 gtggatttac cgctgccaga ctcgcccacc agcgatagcg tttcgccagg ccagagatca
    12961 aaactgactt tctcaacggc atgcacttcc cgcgttacgc gattcaacaa accgctgcgc
    13021 aaagggaaac gggtgacaag attacgcacc cgtaaaacag gttcgccatc caccaccgtt
    13081 ttctgctcga tggggggttc ctgtttcgct ggatgttcaa gcgatatcaa tgggaaacgt
    13141 cggggataat ctaacccttt catcgcacca agttgcggaa cagcagctaa cagcgcacgg
    13201 gtgtaaggat gttgcggtgc atgaaaaatc tgttcgacgg tacccgtttc caccgcctcg
    13261 ccctgataca tcaccagtac ccgatcggca atctctgcca ccacgcccat atcgtgagtg
    13321 ataaagataa cgcccatcga catctctttt tgcaatactt tgattaattg caggatctgc
    13381 gcctgaatag tgacatccag cgcggtggtt ggctcatcgg caatcagcac cgccgggcgg
    13441 catgacagcg ccatcgcaat catcactcgc tggcgcatcc cgccagagag ttgatgcgaa
    13501 taacgtgaaa gaatggtttg tgcctccgga atgcgtacct gatccagcat ccgcttcgcc
    13561 tcgaccatcg cttcttcacg actggcgttc tgatgcagac gaattgattc ggcaatctgt
    13621 tcacccacag taaatactgg gttcagcgat gtcatcggct cctgaaatat catcgccata
    13681 tccgcaccgc gcacatggcg catttgtgca gcgctctgct cgctaagttc aatcacatca
    13741 cgactgcgcc gccgcaacag cattttatcg cactgtacta aaccgcccgc ctgttccaac
    13801 agacgcatca atgccagcgc agtcactgac ttaccggagc cggattcgcc tacaattgcc
    13861 agcgtctcac cgcgttgcag actaaaagag agattgcgga ccgcagctat tttctgctgg
    13921 tcctgcataa aggcaatatt aagattttca accgccagca cattaccggc atcaagttca
    13981 tcactgtgtg gcaacggtgt cccctttttc acggtagata ccggtggttg gcgtatcgcc
    14041 tgcgtagccc caggcgcgat acattccttc ggtgttaaac ggtagcgcga cattcccttc
    14101 atggtcgata gcgattaagc caccgctacc gccaagcgca gggagttttt ccattactac
    14161 ccgctcgcag gcttccgcga gacttaatcc gccgtaatcc attaacgcgg cgatgtcata
    14221 tgccgccagc gcgcggatga agacttcgcc tgtgccggta caagaaaccg ccacactggc
    14281 gttattggcg tagcatccgg cacccactaa gggactatcg ccaactcgtc cgggtaattt
    14341 attggtcatt ccgcctgtgg acgtggctgc cgccagattg ccgtctaaat ccaacgccac
    14401 ggcccccacg gtgcccattt tctgtttttc atccagtggc gcaccgctat ggtcgaggac
    14461 tgttgcccct tcctcgcgcg ctgccagtag ttgttcataa cgcaaaggcg tggagaaaat
    14521 ctccggtgag acgctctcca tgccatgagc aaacgcaaaa ttttctgccc cttcgccaat
    14581 catcatcaca tgcgggcttt gctccatcac cagccgggcg gcaagaaccg gattacgcag
    14641 atgactaacg cccgccaccg caccggcttt cagggtgtta ccatccatca cacaggcgtc
    14701 cagttcatgg gtttcatcac gcgtaaagac agcgccaatt ccggcgttaa acagtggaca
    14761 ctcttccagc agacgcaccg cttccgtcac cacatccagc gcactttcgc cggcttccag
    14821 cattttctgc ccggtttcaa caatggcaga caacgcctcg atgtagcgta attcctgttg
    14881 cagactcatc tgcgcgcggc taattgcacc tgcgccacca tgaattgcaa tgactgcttt
    14941 gcccatagta ttcgtccatt aagggtggct cgccactttt gctataaata tcagaatttt
    15001 tatgaattac tcattcaatt tttgagtata gaaagatgga tatggcatgt aaaggcagga
    15061 gccaacgcta cgcccgaggt ggcatacagt ctatggcgtt tttctgacat aataggcaaa
    15121 ttcgattttg cctccgcagg agtgttttca tggaatttac caccggattg atgtcgctcg
    15181 acaccgcgct taatgagatg ctttctcgcg ttaccccact gaccacccag gaaacgctgc
    15241 cactggttca gtgttttggt cgtattctgg cgagcgatgt cgtttcgcca ctggatgtcc
    15301 cggggtttga taactccgct atggacggct acgcggtgcg tttagccgat attgcctccg
    15361 ggcaaccgct gcccgttgcc ggtaaatcct ttgccggtca gccataccat ggtgaatggc
    15421 ctgcgggtac ctgcattcgt attatgaccg gtgcgccggt gccggaaggc tgcgaagcgg
    15481 tggtgatgca ggagcagact gaacaaacgg acaatggcgt gcgttttact gctgaagtgc
    15541 gtagcgggca aaatattcgc cgtcgcggtg aagatatctc tgcaggtgcg gttgttttcc
    15601 cggcgggaac tcgcctgact accgcagagc tgccagtaat tgcttcactg gggattgccg
    15661 aagttccggt gattcgtaaa gtgcgtgtag cgcttttttc taccggtgat gaactccagt
    15721 tgcccggtca gccgctgggc gacggccaaa tctacgacac caaccgtctc gccgtacacc
    15781 tgatgttaga acagctgggt tgcgaggtaa tcaacttagg aattatccgc gacgatccac
    15841 atgccctgcg cgccgcattt attgaagccg acagccaggc ggatgtagtg atcagttccg
    15901 gcggtgtttc agtgggtgaa gcggattaca ccaaaacgat tcttgaagag ctgggggaga
    15961 tcgccttctg gaagctggcg attaaaccag gtaaaccgtt cgcattcggt aaactcagca
    16021 atagctggtt ctgcggcctg ccgggcaacc cggtttcagc gacgctgacc ttctatcaac
    16081 tggtacagcc tttgctggca aaactaagcg gcaacaccgc cagcggcctg cccgcgcgcc
    16141 agcgcgtacg cacagcgtcc cgcctgaaaa aaacgccagg acgtcttgat ttccagcgcg
    16201 gcgtgctgca acgcaacgcc gacggtgaac ttgaagtgac gaccaccgga catcagggtt
    16261 cacatatatt tagctccttt agtctcggca actgctttat cgtgctggaa cgcgatcgcg
    16321 gcaatgtgga cgtgggcgaa tgggtggaag tagaaccgtt taacgcgttg ttcggaggcc
    16381 tgtaatggcg gaactcagcg atcaggagat gctgcgctac aaccggcaaa tcattctgcg
    16441 cggttttgat tttgacggtc aggaggcgct aaaagattct cgtgtgctgg tagtcggcct
    16501 cggtggactc ggctgcgcag cttcgcagta tctggcaagc gccggtgtcg gcaacctgac
    16561 gctgctcgac ttcgacacgg tttcgctctc gaatctgcaa cgccagacac tgcacagtga
    16621 tgccacggtc gggcaaccga aggtggaatc tgcccgtgac gccctgacgc ggatcaaccc
    16681 acatatcgcg attacgccag tcaatgcact gctagatgac gcagaacttg cagcattgat
    16741 tgctgaacac gatctggtgc tcgactgtac ggataacgtt gcggtacgta atcaactgaa
    16801 cgcaggctgt tttgccgcga aggtgccgct ggtttccggc gcggcaattc gtatggaagg
    16861 gcaaatcacg gtcttcactt atcaggacgg cgaaccgtgc tatcgctgcc ttagccgttt
    16921 gtttggcgag aacgcgttaa cctgcgtaga agcaggcgtg atggcaccgt tgatcggcgt
    16981 aattggttcg ttgcaggcaa tggaagcgat caaactgctg gcaggttatg gcaaaccggc
    17041 cagcgggaaa atcgtcatgt acgatgcgat gacctgtcag tttcgcgaaa tgaaactgat
    17101 gcgcaatccg gggtgtgagg tgtgcgggca gtaattgcca cctccaggcc tgataagacg
    17161 cgacaagcgt cgcatcaggc atttgctcca gacgccagat gcggcgtgaa cgccttatct
    17221 gccctacggt tcgggcacag atttccaagc gcggcaggca tcggtgcact ccgccacgtc
    17281 atcagcaatt tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc aaacgctccc
    17341 tgccagtcct gctcaaactt cgccacagcg gcttccaccg ccggatagct aatcatctgt
    17401 tgtgccacat ccagtggcag agtaattgat tcacatccgg ccagtaagca gtccagcgcc
    17461 tgacgcgggg ttttgaaact cgctgccagc actttcgcct gcggcgcatg cattttcaat
    17521 aactggtgta agtcggtcac agtctgaatg ccgctaccgc cctgagcatc aatacgatta
    17581 acgtaaggcg caacatattc cgcacctgcc agcgccgaca gcagcccttg tgccgcgcca
    17641 tataccgcgg ttcccagcgt cggaatccct tccgctttta acatcttaat cgctgccagc
    17701 ccctcggctg tcaccggaac tttcaccacg atatccgcaa taatagaacg cagcttaagc
    17761 gcgtcattca ccatcccttc agcagtggta gccattacct gggcaaacag acgcccctga
    17821 ccgcccatcg cttcatgaag ttgcggaagc acaacatcca gcggtttttt acccgcggcg
    17881 ataatgcttg ggttagtggt cacacccgcc agcggaaaaa tacgtgacag cgccttcacc
    17941 gcaacaacgt ctgaagtatc cagatacagt tccataacca tcctcaaaat gtactttttg
    18001 tctgaatgta gactatcacg ggaaaactct ctgaacagtt gacgcacatc aatctaactt
    18061 tcattcgaaa gtattttaat gtttgaacga aacagagagg caactatgat tttcaatatt
    18121 cagcgctact cgacccatga tggccccggt atccgcacgg tcgtatttct taaaggctgt
    18181 tcgctgggct gccgctggtg tcagaacccg gaaagccgcg cccgtacgca ggatctgctg
    18241 tatgacgcac gactgtgtct ggaaggctgc gagctgtgcg ctaaggccgc gccggaagtg
    18301 attgagcgcg cgctgaatgg tttgcttatt catcgggaaa agttaacccc ggagcatctg
    18361 acggcgttaa ccgactgctg tccgacacag gcgttaaccg tgtgtggtga agtgaaaagc
    18421 gttgaggaga tcatggcgac cgttctgcgc gataaaccgt tttacgatcg cagcggcggc
    18481 ggcttaacgc tttcgggtgg tgaacccttc atgcagccgg aaatggcgac ggcgctgctg
    18541 caagcctgcc acgaggcagg cattcatact gcggtagaaa cctgtctaca tgtgccgtgg
    18601 aaatatatcg ccccttctct gccctatatc gatctgtttc ttgccgattt aaaacacgtt
    18661 gccgacgcgc cgtttaaaca gtggaccgac ggtaacgccg ccagggtgct ggataacctg
    18721 aaaaaactcg ccgcagcggg caaaaaaatc attatccgcg tgccgctgat tcagggcttt
    18781 aatgccgacg aaacctctgt aaaagccatt accgattttg ccgccgacga gctgcacgtt
    18841 ggcgaaattc attttctgcc ctaccacacg ctgggcatca acaaatatca cttacttaat
    18901 ctgccctatg acgccccgga aaaaccgctt gatgcgccag agctgctcga ttttgcccag
    18961 cagtatgcct gccagaaagg gttaaccgcg accttacgag gataacaacc atgaccacac
    19021 tgaaactgga cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg gtgcatattg
    19081 tgaaaccgcc agtctgtacc gagcgcgcgc agcactatac cgagatgtat caacaacatc
    19141 tcgataagcc gatcccggta cgtcgcgcgc tggcactggc gcatcacctg gcgaatcgca
    19201 ccatctggat caaacactat gagttgatca ttggcaacca ggcaagcgaa gttcgcgccg
    19261 cgccgatctt cccggaatat actgtctcgt ggatcgaaaa agagattgat gatctggcag
    19321 atcgtcccgg tgctggcttt gcggtgagcg aagagaacaa acgtgttctg catgaagtgt
    19381 gcccgtggtg gcgcggtcag acggtacagg atcgctgcta tggcatgttt accgatgagc
    19441 aaaaaggtct gctggcgacc ggcatcatta aagcggaagg caatatgacc tccggcgatg
    19501 cgcacctggc ggtcaatttc ccgctgctgc tggaaaaagg gcttgatggt ctgcgtgaga
    19561 aagtagcgga acgtcgctcg cgcatcaatc tgacggtgct ggaagatttg cacggcgaac
    19621 agttcctgaa agcgattgat atcgtgctgg tggcagtcag tgaacacatt gaacgtttcg
    19681 ctgccctggc gcgtgcaatg gccgcgaccg aaacccgcga aagccgtcgc gatgaactgc
    19741 tggcgatggc agaaaactgc gatcttatcg cccaccagcc gccgcagact ttctggcagg
    19801 cgctgcaact gtgttacttc atccagttga ttttgcagat tgaatctaac ggtcactcgg
    19861 tgtcgtttgg tcgtatggat cagtatctct acccgtacta tcgccgcgac gttgaactga
    19921 accagacgct ggatcgcgaa cacgccatcg aattgctgca tagctgctgg ttgaaattgc
    19981 tggaagtgaa caaaatccgc tccggctccc actcaaaagc ctctgcggga agtccgctgt
    20041 atcagaacgt caccattggc ggacaaaatc tggttgatgg tcaaccaatg gacgcggtga
    20101 atccactctc ttacgcgatc ctcgaatcct gcggtcgcct acgttccact cagcctaacc
    20161 tcagcgtgcg ttaccatgca ggaatgagta acgatttcct cgacgcctgc gtacaggtga
    20221 tccgctgcgg cttcgggatg ccggcattca acaacgacga aatcgtgatc ccggaattta
    20281 ttaaactcgg tattgaaccg caggacgctt atgactacgc agcgattggt tgtatagaaa
    20341 ccgccgtcgg tggcaaatgg ggctatcgct gtaccggcat gagctttatc aacttcgccc
    20401 gcgtgatgct ggcggcgctg gaaggcgggc gtgatgccac cagcggcaaa gtgttcctgc
    20461 cacaagaaaa agcgttgtcg gcaggtaact tcaacaactt cgatgaagtg atggccgcgt
    20521 gggatacgca aatccgttac tacacgcgta aatcaatcga aatcgaatat gtcgtcgaca
    20581 ccatgctgga agagaacgtg cacgatattc tctgctcggc gttggtggat gactgtattg
    20641 agcgagcgaa aagtatcaag caaggcggcg cgaaatatga ctgggtttct ggcctgcagg
    20701 tcggtattgc caacctcggc aacagcctgg cggcagtgaa gaaactggtg tttgaacagg
    20761 gcgcgattgg tcagcaacag ctggctgccg cactggcgga tgacttcgat ggcctgactc
    20821 atgagcaact gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac gacgatgata
    20881 ccgtcgatac gctgctggct cgcgcttatc agacctatat cgacgaactg aaacagtacc
    20941 ataacccacg ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt acgtcgtcta
    21001 tctccgctaa cgtaccgttt ggcgcgcaaa ctatggcaac gccggacggg cgtaaagccc
    21061 acaccccgct ggcagaaggc gctagtccgg cctctggtac tgaccatctc ggccctactg
    21121 cggtcattgg ctcagtgggt aaactgccta cggcagcgat tctcggcggc gtgttgctca
    21181 accagaaact gaatccggca acgctggaga acgaatctga caagcagaaa ctgatgatcc
    21241 tgctgcgtac cttctttgaa gtgcataaag gctggcatat tcagtacaac atcgtttccc
    21301 gcgaaacgct gctggaggcg aaaaaacatc cggatcagta ccgcgatctg gtagtgcgtg
    21361 tcgcgggtta ttccgccttc ttcaccgcgc tctctccaga cgctcaggac gatatcatcg
    21421 cccgtactga acatatgctg taatctcttc ggcccggctg cggcgtcgcc gggctaaatc
    21481 attcacatca tcaatttcat ccttactttc attcgaaata taatttgtgc tctgcgtcac
    21541 attgttatta cactgttacg gtttcatttt cgagccagga gcacacctat gagcgtaaaa
    21601 gttatcgtca cagatatgga cggtactttt cttaacgacg ccaaaacgta caaccaacca
    21661 cgttttatgg cgcaatatca ggaactgaaa aagcgcggca ttaagttcgt tgtcgccagc
    21721 ggtaatcagt attaccagct tatttcgttc tttcctgagc taaaggatga gatctctttt
    21781 gtcgcggaaa acggcgcact ggtttacgaa catggcaagc aactgttcca cggcgaactg
    21841 acccgacatg aatcgcggat tgttattggc gagttgctaa aagataagca actcaatttt
    21901 gtcgcctgcg gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc atttgtcgca
    21961 ctgatggcaa aacactacca tcgcctgaaa cctgtaaaag attatcagga gattgacgac
    22021 gtactgttca agttttcgct caacctgccg gatgaacaaa tcccgttagt gatcgacaaa
    22081 ctacacatag cgctggatgg cattatgaaa cccgtcacca gtggttttgg ctttatcgac
    22141 ctgattattc ccggtctaca taaagcaaac ggtatttcgc ggttactgaa acgctgggat
    22201 ctgtcaccgc aaaatgtggt agcgattggc gacagcggta acgatgcgga gatgctgaaa
    22261 atggcgcgtt attcctttgc gatgggcaat gctgcggaaa acattaaaca aatcgcccgt
    22321 tacgctaccg atgataataa tcatgaaggc gcgctgaatg tgattcaggc tgtgctggat
    22381 aacacatccc cttttaacag ctgacctctc taccggagcc atgcgctccg gttttccctt
    22441 ctttttacat cctgtacatc acatcaaatc attaaactgc attaacttat ttataacttg
    22501 tattgtcaca tgcaaatcag catagtttcg aatgaaagtt aatgcgaggt ggttatggct
    22561 tctactttta ccagcgacac attgcctgcc gatcacaaag cagctatccg tcagatgaag
    22621 cacgcgctgc gggcgcagct tggcgatgtc cagcagatct ttaatcagct aagcgatgac
    22681 attgccacgc gagtggctga aatcaacgca ctcaaagcac agggcgatgc cgtctggccg
    22741 gtgctgtctt atgccgatat caaagcaggt catgttactg cagagcagcg cgaacagatt
    22801 aaacgtcgcg gttgtgcggt gataaaaggc catttccccc gcgaacaagc gctaggctgg
    22861 gatcagtcga tgctggacta tctgaaccgc aaccgctttg acgaggtcta caaaggcccc
    22921 ggcgataatt tcttcgggac gctcagcgct tcacgtcccg agatttaccc catctactgg
    22981 tcgcaggcgc aaatgcaggc ccgccagagt gaagaaatgg cgaatgcgca gtcgtttctc
    23041 aatcgtctgt ggacatttga aagtgatgga aagcaatggt ttaacccgga tgtgagcgtc
    23101 atctaccctg accgtatccg ccgccttccg cccggaacga cctccaaagg tcttggagcg
    23161 cataccgact ccggggcgct ggaacgctgg ctgcttccag cgtatcagca cgttttcgcc
    23221 aacgtcttta atggcaatct ggcgaaatac gatccctggc atgcggcaca tcgtacggaa
    23281 gttgaagagt acacggtgga caacaccacc aaatgttccg tgtttcggac attccagggc
    23341 tggactgcgc tctcggatat gctgcctggt caggggttgc tgcacgtcgt gcccattcct
    23401 gaagccatgg cgtacgtact gttacgtccg ctgcttgatg atgtgccgga ggatgaactg
    23461 tgcggcgtag cgcccggaag agtgttgccg gtatcagagc aatggcatcc actgttaatt
    23521 gaggcgttaa ccagcattcc aaaactcgag gccggagact ccgtctggtg gcactgcgac
    23581 gtcatccatt ccgttgcccc cgttgaaaat caacagggtt ggggcaacgt gatgtacatt
    23641 cctgcggcac cgatgtgcga gaaaaatctt gcctacgcgc acaaggtgaa ggccgcactg
    23701 gaaaaaggcg catcgccggg cgacttcccg cgcgaggact atgaaacaaa ctgggaagga
    23761 cgctttacgc ttgccgacct caacattcac ggtaagcgag cgttgggcat ggatgtttga
    23821 gagttaatgg aagtgaattg atgtgggatt aagaaaatcg attcgacgtt actcagattg
    23881 ctgacaagtg cgctttctta atgcctgatg cggcgttaac gccttatccg gcctacaaag
    23941 ttctgcagat tcaatatatt acaggtactg cgtaggcctg ataagcgtag cgcatcaggc
    24001 tattttgcat ttgtcatcag ccgtcgtcac cccgacagta tcggggtgac ggataatcac
    24061 ttactcgatc cctttactac gcaggtaatc ttcgtaatta ccgctaaagt cgatcacgcg
    24121 ttccggggtg atttccagaa tgcgggtcgc cagggagctt acgaactcac ggtcgtgtga
    24181 aacaaagatc agcgtgccct gatacagttc cagcgccatg ttcagtgact cgatggattc
    24241 catatccagg tggttggtcg gttcgtccat gatcagaatg ttcggcttct gcatcattaa
    24301 cttaccaaac agcatccgcc ctttttcccc accggaaagc actttagctg gctttttgat
    24361 gtcgtcctgg ctgaacagca aacgaccgag aatgctgcgt accgcctgct cgtcatcgcc
    24421 ttcctgcttc cactgactca tccattcgaa cacggtcaga tcattttcaa actcatattc
    24481 gtggtcctgg gcgtagtaac caatgcgcgc gttttcagac cattttacgg tgccgctgtc
    24541 cggttgcaga tcgcccacca gcgttttcag cagcgttgat ttaccgacgc cgttggtacc
    24601 cagtaccgca agtttttcac cgacctccag cagcaggttg agatttttaa acagtggacc
    24661 gttatcaaac cctttggtca gaccttccac ttccagcgcg ttacggaaca gtttcttatc
    24721 ctgttcaaaa cggatgaacg ggttctgacg gctggaggct ttcacctctt ccagtttgat
    24781 tttatcaatc tggcgcgcgc gcgaagttgc ctggcgagat ttcgaggcgt tggcgctaaa
    24841 gcggctaacg aaagattgca actcagcaat ctgcgctttc ttcttggcgt tatcggccag
    24901 cagacgttca cgcgcctggg tcgccgccgt catgtactca tcgtagttac ccggataaac
    24961 gcgcagctcg ccgtaatcca gatccgccat gtgggtacag accatgttaa ggaagtgacg
    25021 gtcgtgcgag atgatgatca tggtgctgtc acgctcgttc agcacctgtt ccagccagcg
    25081 aatggtgtcg atgtcgaggt tgttggtcgg ttcgtcgagc aggagaatat ccggctcagc
    25141 aaacagcgcc tgcgccagaa gcacacgcag cttccagcca ggagcaactt cactcatcgg
    25201 gccgtagtgc tgttccactg gaattcccac gccaagcaac agttcaccgg cgcgagcttc
    25261 cgcagagtaa ccgtccattt cgccgtattt cacttccaga tcggccactt tatagccgtc
    25321 ttcttcactc atttccggca aagcatagat gcggtcgcgc tcctgcttca cttcccacaa
    25381 ctctttatgc cccatgatca ccgtatccag cacagtgaac ccttcaaagg caaactgatc
    25441 ctgacgcagc ttaccaatgc gctcgttggg atcgagggaa acgttaccca gcgtcggctc
    25501 aaggtcgccg ccgagaatct tcataaaggt ggatttacca ctaccgttcg cgccaatcag
    25561 gccgtaacgg ttgccgccgc cgaatttgac ggaaatgttt tcaaacaacg gcttactgcc
    25621 gaactgcatg gtgacgttac tggaaactaa cacaggcgta tcctgaaaag agatatgaca
    25681 aaccgcgcat tatgccataa gtctgaaaga agatcctgct cctcgcttaa ggtctacact
    25741 caagccaaag gcaaaaaatg tgatttcgta cacatctgat ttcactgtga gctggaatga
    25801 acttataatg cgcttccaat actctaatat tctcaaccca atggcctgcc aggcacaaaa
    25861 tctcgcttaa catgaatatg aaattgaaaa cattattcgc agcggccttc gctgttgtcg
    25921 gcttttgcag taccgcctct gcggtaactt atcctctgcc aaccgacggg agtcgcctgg
    25981 ttggtcagaa tcaggtgatc accattcctg aaggtaacac tcagccgctg gagtattttg
    26041 ccgcggagta ccagatggga ctttccaata tgatggaagc gaacccgggt gtggatacct
    26101 tcctgccgaa aggcggtact gtactgaaca ttccgcagca gctgatcctg ccggataccg
    26161 ttcatgaagg catcgtcatt aacagtgcag agatgcgtct gtattactat ccgaaaggga
    26221 ccaacaccgt tatcgtgctg ccgatcggca ttggtcagtt aggcaaagat acgcctatca
    26281 actggaccac caaagttgag cataagaaag caggcccgac ctggacgccg accgccaaaa
    26341 tgcacgcaga gtaccgcgct gcgggcgaac cgctaccggc tgtcgttcct gcaggtccgg
    26401 ataacccgat gggtctgtat gcactctaca tcggtcgcct gtatgctatc catggcacca
    26461 acgccaactt cggtatcggc ctgcgtgtaa gtcatggttg tgtgcgtcta cgtaacgaag
    26521 acatcaaatt cctgttcgag aaagtaccgg tcggtacccg cgtacagttt attgatgagc
    26581 cggtaaaagc gaccaccgag ccagacggca gccgttatat tgaagtccat aacccgctgt
    26641 ctaccaccga agcccagttt gaaggtcagg aaatcgtgcc aattaccctg acgaagagcg
    26701 tgcagacagt gaccggtcag ccagatgttg accaggttgt tcttgatgaa gcgatcaaaa
    26761 atcgttccgg gatgccggtt cgcctgaatt aatcgtcaaa acgtaaaaca aaggcggact
    26821 cataatccgc cttttttatt tgccagacct taccaggccg ggatcataac taacaaaacg
    26881 tatcccacca gcgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
    26941 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc tggtaataaa
    27001 ccaaagcccc ccacgtttac cgcccatgcc agtaataaag acggaggtac ataattgagc
    27061 agcaaaatgg tactcggcac gttactgatc acctgcgata aaccgactgc cgttaaccat
    27121 aacccgggtt cagataaatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
    27181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcaacgt ccagtccaca
    27241 ctcaacacca cgcgacgcgc cagcagcaca aagcccgccg ccacaatcac cagtccccac
    27301 agctcttgtt tgaactccag cgccgtcaga aaaacgatat acagccccag acaattccac
    27361 accagccgcg gtttccactc cggtgtttgc attcccgtat ggtattgcag cgcctttcca
    27421 gggaaacaac accagcacaa cagcaggagt gtcagcatca ttgcgccagc cagcggtgcc
    27481 atttgggaaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
    27541 gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
    27601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
    27661 aacagcgcga catcgttggt ctgaaaggta gaaagcagcg ccgccgccag caccataaac
    27721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
    27781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
    27841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
    27901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
    27961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
    28021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
    28081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
    28141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
    28201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
    28261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
    28321 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
    28381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
    28441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
    28501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
    28561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
    28621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
    28681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttcagat
    28741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttt atgcaggcat aacgcgtttt
    28801 ggtctgaaaa aaaccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt
    28861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
    28921 tcgcggatgc ggaccccttt cccctcttca cgcactcttg caggtattga cccttgacgc
    28981 cagggtaagc acatggcgtt tgttaccata gtggcatatg aatttaaccc tcaaagaatc
    29041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
    29101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
    29161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtgtcag
    29221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaatttcaa
    29281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
    29341 tccgtggtac agctacctgg tcggcttatt tatttttgcg ctcggcgtaa tagcaatcag
    29401 gcgaaaaaaa gagaatgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
    29461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggtg gcgtatttaa
    29521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
    29581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
    29641 ggtcactgcc gttaagccga agtatcagac ctatgtggta gtgatcggtg aaagcgcgcg
    29701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
    29761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
    29821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
    29881 agcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa tcggcgaata
    29941 cgatactgct atcgccagca tcgccaaacg tgcagatgaa gtgtacttcc tgaaagaagg
    30001 taattttgaa gcagataaaa acacgaaaga cgaagcgtta ctggatatga ccgctcaagt
    30061 gctggcgcaa gagcactcgc aaccgcagct gattgttctg catctgatgg gctcgcatcc
    30121 gcaggcctgc gacaggacac aaggcaaata caaaaccttt gtgcaatcga aagaaacgtc
    30181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
    30241 acgcaacagc ggcagcagct tctctctggt ttacttttct gaccacggtc tggcctttaa
    30301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aaatatcagc aaaatttcca
    30361 ggtgcctttt atggtcattt ccagcggcga taaagcgcat cgggtgatta aagcccgccg
    30421 ctcagccaat gacttcttag gctttttctc gcagtggacg ggaattaaag cgaaggaaat
    30481 aaatatcaaa tacccgttta tatctgagaa gaaagccggg ccgatataca tcaccaactt
    30541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaaacctta
    30601 aaaacaaaaa tccgccccga gaggcggatt tttatatcac caaagtgatt agaagcggta
    30661 accaacaccg gcaatccagg tgcctacgtc aacgctacga atacggctct gctcgtaaga
    30721 gaagtccaga gcaacgtttt ccatcgggtt gaactgcaga ccagcaccgt aggagaaacc
    30781 gtagtcgctg gtgtcgtgtt tgtaggtcgg gtattcagtg gtctggaatt taccataacc
    30841 cacacccact acaccgtaga tgcttgccca gtcgttaatg cggtaagccg gaccagcagt
    30901 gatgccgtag tactggtttt tgttgtagtc accagagctt gcagtacggc ttttctcggt
    30961 gtaagtgaaa gaaccgatca cacccagcgg gctgttgtct tcttcatagc ggtatttcag
    31021 gtttaaaccg cccattttgt tcatttggcc ctgagcgtcg ctctgtgcgt aaccgccagt
    31081 tacggtagaa gtcgcagcta cggaagtacc tgcggtgaaa gccagaactg cggccagtgc
    31141 tgaaagacat gcaatttttt tcataaccac ctcaaatgtg attcaaataa gtcctaagtt
    31201 ttaaatatat caaaaattaa tgggaaactc ttcgcgattt gtgatgtcta acgggccatt
    31261 tcatgtaaca gaacgtttcc atacaccgct atccatctaa atttaaatca ctttttcaga
    31321 gaactgcgta agtattacgc atgttttccc tgtcattcat ccagattatt cctaatcacc
    31381 agactaatga ttccatcaat cctggcgcat tttagtcaaa acgggggaaa attttttcaa
    31441 caaatgctca accagcattg ggtatatcca gtacactcca cgctttactt aagtctagat
    31501 atttgtggga gaaaggatgc ctggttcatt acgtaaaatg ccggtctggt taccaatagt
    31561 catattactc gttgccatgg cgtctattca gggtggagcc tcgttagcta agtcactttt
    31621 tcctctggtg ggcgcaccgg gtgtcactgc gctgcgtctg gcattaggaa cgctgatcct
    31681 catcgcgttc tttaagccat ggcgactgcg ctttgccaaa gagcaacggt taccgctgtt
    31741 gttttacggc gtttcgctgg gtgggatgaa ttatcttttt tatctttcta ttcagacagt
    31801 accgctgggt attgcggtgg cgctggagtt caccggacca ctggcggtgg cgctgttctc
    31861 ttctcgtcgc ccggtagatt tcgtctgggt tgtgctggcg gttcttggtc tgtggttcct
    31921 gctaccgctg gggcaagacg tttcccatgt cgatttaacc ggctgtgcgc tggcactggg
    31981 ggccggggct tgttgggcta tttacatttt aagtgggcaa cgcgcaggag cggaacatgg
    32041 ccctgcgacg gtggcaattg gttcgttgat tgcagcgtta attttcgtgc caattggagc
    32101 gcttcaggct ggtgaagcac tctggcactg gtcggttatt ccattgggtc tggctgtcgc
    32161 tattctctcg accgctctgc cttattcgct ggaaatgatt gccctcaccc gtttgccaac
    32221 acggacattt ggtacgctga tgagcatgga accggcgctg gctgccgttt ccgggatgat
    32281 tttcctcgga gaaacactga cacccataca gctgctggcg ctcggcgcta tcatcgccgc
    32341 ttcaatgggg tctacgctga cagtacgcaa agagagcaaa ataaaagaat tagacattaa
    32401 ttaaatttac atttctgtat ggttatgcat aaccatgcag aatttctcgc tacttttcct
    32461 ctacaccgtc tttatatatc gaattatgca aaagcatatt tattccgaaa attcctagcg
    32521 agcagataaa taagaattgt tcttatcaat atatctaact cattgaatct ttattagttt
    32581 tgttttttac gcttgttacc actattagtg tgataggaac agccagaata gcggaacaca
    32641 tagccggtgc tatacttagt ctcgttaatt actgggacat aacatcaaga ggatatgaaa
    32701 ttatgagtac cgctaaatta gttaaatcaa aagcgaccaa tctgctttat acccgcaacg
    32761 atgtctccga cagcgagaaa aaagcaacag tagagttgct gaatcgccag gttatccagt
    32821 ttattgatct ttctttgatt accaaacaag cgcactggaa catgcgcggc gctaacttca
    32881 ttgccgtaca tgaaatgctg gatggcttcc gcaccgcact gatcgatcat ctggatacca
    32941 tggcagaacg tgccgtgcag ctgggcggtg tagctctggg gaccactcaa gttatcaaca
    33001 gcaaaacccc gctgaaaagt tacccgctgg acatccacaa cgttcaggat cacctgaaag
    33061 aactggctga ccgttacgca atcgtcgcta atgacgtacg caaagcgatt ggcgaagcga
    33121 aagatgacga caccgcagat atcctgaccg ccgcgtctcg cgacctggat aaattcctgt
    33181 ggtttatcga gtctaacatc gaataaatcc atcgctgatg gtgcagaact ttagtacccg
    33241 ataaaagcgg cttcctgaca ggaggccgtt ttgttttgca gcccacctca acgcacttat
    33301 ttagtgcatc catctgctat ccccagctga ttaagtaaat tttttgtatc cacatcatca
    33361 cacaaccgtt acataaagat tgttttttca tcaggtttta cgctaaataa tcactgtgtt
    33421 gagtgcacaa ttttagcgca ccagattggt gccccagaat ggtgcatctt cagggtattg
    33481 ctctataaat cgtgcatcac gtttttgccg catctcgaaa aatcaatgag ttgcaaaact
    33541 ggcacgattt tttcatatat gtgaatgtca cgcaggggat cgtcccgtgg atagaaaaaa
    33601 ggaaatgcta tgaagtctgt attaaaagtt tcactggctg cactgaccct ggcttttgcg
    33661 gtttcttctc atgccgcgga taaaaaatta gttgtcgcga cggataccgc cttcgttccg
    33721 tttgaattta aacagggcga taaatatgtg ggctttgacg ttgatctgtg ggctgccatc
    33781 gctaaagagc tgaagctgga ttacgaactg aagccgatgg atttcagtgg gatcattccg
    33841 gcactgcaaa ccaaaaacgt cgatctggcg ctggcgggca ttaccatcac cgacgagcgt
    33901 aaaaaagcga tcgatttctc tgacggctac tacaaaagcg gcctgttagt gatggtgaaa
    33961 gctaacaata acgatgtgaa aagcgtgaaa gatctcgacg ggaaagtggt tgctgtgaag
    34021 agcggtactg gctccgttga ttacgcgaaa gcaaacatca aaactaaaga tctgcgtcag
    34081 ttcccgaaca tcgataacgc ctatatggaa ctgggcacca accgcgcaga cgccgttctg
    34141 cacgatacgc caaacattct gtacttcatc aaaaccgccg gtaacggtca gttcaaagcg
    34201 gtaggtgact ctctggaagc gcagcaatac ggtattgcgt tcccgaaagg tagcgacgag
    34261 ctgcgtgaca aagtcaacgg cgcgttgaaa accctgcgcg agaacggaac ttacaacgaa
    34321 gtctacaaaa aatggttcag tactgaaccg aaataataac gctacacctg taaagcgcac
    34381 tggcagttcc ctctcccccc tggggagagg attagggtga ggggcgcaaa cccgctccgg
    34441 ggccattaat taccctgaat ttgattattt acaccacggt aacaggaaca acatatgcag
    34501 tttgactgga gtgccatctg gcctgccatt ccgcttctga ttgaaggtgc caaaatgacc
    34561 ctgtggattt cggtcctcgg tctggctggc ggtctggtaa tcggattgct ggcaggtttt
    34621 gcacgcacct tcggtggttg gatagccaac cacgtcgcgc tggtctttat tgaagtgatc
    34681 cgcggcacac ctatcgtcgt ccaggtgatg tttatctatt tcgccctgcc gatggcgttt
    34741 aacgacttac gcatcgaccc atttactgcg gcggtggtca ccatcatgat caactccggc
    34801 gcgtacattg cggaaatcac gcgtggtgcg gtgctgtcta tccacaaagg ttttcgtgaa
    34861 gcaggactgg cgctcggtct ttcacgttgg gaaaccattc gctacgtcat tttaccgctg
    34921 gcactgcgtc gtatgctgcc gccgctgggt aaccagtgga tcatcagcat taaagacacc
    34981 tcactgttta ttgttatcgg cgtggcggaa ctgacccgtc aggggcaaga aattattgcc
    35041 ggtaacttcc gcgcccttga gatctggagc gccgtggcgg tgttctatct gattattacc
    35101 ctggtgctga gctttattct gcgtcgtctg gaaagaagga tgaaaatcct gtgattgaat
    35161 ttaaaaacgt ctccaagcac tttggcccaa cccaggtgct gcacaatatc gatttgaaca
    35221 ttgcccaggg cgaagtcgtg gtgattatcg ggccgtccgg ttccggtaaa tcgaccctgc
    35281 tgcgctgcat caacaaactg gaagaaatca cctccggcga tctgattgtc gatggcctga
    35341 aggttaacga tccgaaagtt gacgagcgcc tgattcgcca ggaagcaggt atggtgttcc
    35401 agcagtttta cctcttcccg catctgacgg cgctgggaaa cgtcatgttt ggtccgctac
    35461 gcgtgcgtgg cgcgaacaaa gaagaggcgg aaaaactggc gcgtgagctg ctggcgaaag
    35521 tcggtctggc agaacgtgca catcactacc cttccgaact ttctggtggt caacagcagc
    35581 gtgtggcgat tgcccgcgcg ctggcggtga agccgaaaat gatgctgttt gatgaaccga
    35641 cttccgctct tgacccggaa ctgcgccatg aagtgctgaa ggttatgcag gatctggctg
    35701 aagaagggat gacgatggtg atcgtgaccc acgaaatcgg ttttgccgag aaagtagctt
    35761 cgcggctgat ctttatcgac aaaggccgga ttgcggaaga tggcaatccg caggtgttga
    35821 tcaagaaccc gccgagccag cgcttgcagg aatttttgca gcacgtctct taataagaca
    35881 cattgcctga tgcgctacgc ttatcaggcc tacaggatat ctggcaactt attaaaattg
    35941 catgaacttg taggacggat aaggcgttca cgccgcatcc ggcaaaaaag cccgcacgtt
    36001 gtcagcaacc ggcttaatat cccttcctcc ctttcacccg aaagggaggc acaccagatt
    36061 cctctcattt aaaatcgccc ctcccccagc atctatactt atctttttgc tctgttttct
    36121 cactggagga gtcatgcggt ggatcctgtt catcctcttc tgcctgctgg gcgcacctgc
    36181 ccacgcggta tccatacccg gcgttacaac cacaacgaca acggactcaa cgactgaacc
    36241 ggccccggaa ccggatatcg aacaaaaaaa gcggcctatg gcgcactggc ggatgtgctg
    36301 gataacgaca cctcgcgtaa agagttgatc gaccagttgc gtactgtcgc cgcgacgccc
    36361 cctgcggaac cggtcccgaa gatcgtgccg ccgacgctgg ttgaagagca aaccgtgctg
    36421 caaaaggtca ccgaagtcag ccgccattat ggtgaagccc tttccgcccg cttcgggcaa
    36481 ctttatcgca atatcaccgg ctccccgcat aagccattta atccacaaac cttcagcaat
    36541 gcgctgagcc atttttcaat gttagcggta ttagtgtttg gtttttactg gctgattcgc
    36601 ctgtgcgcac tgccgctata tcgcaaaatg ggtcagtggg cgaggcaaaa aaatcgtgag
    36661 cgcagcaact ggttgcagct tccggcgatg attattgggg cgtttattat cgacctgctg
    36721 ttactggccc tgacattatt tgtcggccag gtattaagcg acaacctgaa tgcaggtagt
    36781 cgcaccatcg ctttccaaca aagtttgttt ctcaacgcct ttgccctgat tgaatttttc
    36841 aaagccgtac tacgcctgat tttttgccca aacgtggcgg agctgcgccc gttcacgatt
    36901 caggacgaga gcgcccgtta ctggagtcgc cgcctgagct ggttaagcag cctgattggc
    36961 tatggcctga ttgtggccgt gccgattatc tctaatcagg tgaatgtaca gataggtgcg
    37021 ctggcgaacg tcatcattat gctgtgcatg accgtctggg cgttgtacct gatcttccga
    37081 aataaaaaag agattaccca gcatttgctc aactttgcgg agcattcgct ggcttttttc
    37141 agcctgttta tccgcgcctt tgcgctggtg tggcactggc tggcaagcgc ctattttatc
    37201 gtgctgtttt tcttttcgtt gttcgatccg ggcaacagtc tgaaatttat gatgggtgca
    37261 acggtgcgca gcctggcgat tattggtatc gcagcgtttg tttccggtat gttttcccgc
    37321 tggctggcga aaaccatcac cctctcgcca catactcagc gtaactatcc ggagctacaa
    37381 aaacggctga atggctggct ttcggcggcg ctgaaaacgg cgcgtattct gaccgtctgc
    37441 gtggcagtaa tgttgctgtt gagcgcatgg ggattgttcg atttctggaa ctggctgcaa
    37501 aacggcgcgg ggcagaaaac cgtagatatc ctgatccgca tcgcactcat tcttttcttc
    37561 tcggcggttg gctggacggt gctcgccagt ttgatcgaaa accggctggc ttcggatatt
    37621 catggccgcc cgctacccag cgcccgtacg cgtaccctgc tgacgctgtt ccgtaacgcg
    37681 ctggcggtga ttatcagtac catcaccatc atgattgtgt tgtcggaaat cggcgtcaat
    37741 atcgcgccat tgctggcagg tgccggggca ttaggtctgg ctatctcgtt tggttcgcaa
    37801 acgctggtaa aagatattat caccggggta tttattcagt ttgaaaacgg catgaacact
    37861 ggagatttgg tgactatcgg gccgttgacc ggcacagtgg aacggatgtc gattcgctcc
    37921 gtgggcgtgc gccaggatac cggggcgtat cacatcattc cgtggtcttc gataaccacc
    37981 tttgctaact tcgtccgcgg tattggttcg gtagtggcaa actacgatgt tgatcgccat
    38041 gaagatgctg ataaagccaa tcaggcactg aaagatgcgg taacggaatt gatggaaaac
    38101 gaagaaattc gcgggctgat tattggtgaa ccgaactttg ccgggattgt cggcttaagc
    38161 aataccgcgt ttacactgcg tgtttcgttc accacgctgc cactcaaaca gtggacggta
    38221 cgctttgccc tcgacagcca ggtgaaaaaa catttcgacc tggcgggcgt tcgcgcgcca
    38281 gtgcagactt atcaggtgct gcctgctccg ggcgcgaccc cggctgaacc gctgccgccg
    38341 ggggagccaa cgctttaacg ttggcgatta acaaaacggc ggcgctgctc gtcgttcata
    38401 aaggtccagg caataaagcg actctgcttt tgcccctggg ccatctcttt ttttaccacc
    38461 ttcaccgcgc ccacgtcggt cagagcacga tacaacggcg gtaagttttc accacgagat
    38521 accagcgagg taaaccacat cacctgcttc gcgaagcctt tgctctcttc aatcatcttt
    38581 ttgataaagt tgacttcacc gccttcacac cacaactcct gttgctgacc accaaagttc
    38641 agtgcatcgt ctttgttcag ccccaggtta cggcgtttac gctcactacc tgcccgcgcc
    38701 gcagcggcgg aatcgtggaa tggcgggtta cacaaggtcg cgtcgtattg ctcgtttttg
    38761 tggatgatgc cgttaaaaat cgccccactc tctttttgcc gacgcagacg aatggcgcgg
    38821 ttaagccccg gattagcact gataatcgcc tgcgcactgc ttaacgcctg gctgctggtt
    38881 tcactaccgg taaaacgcca gccatattca tgtacgccaa tcagcggata aatacagttc
    38941 gcaccaacgc cgatatccag aatgctggca ttcgctggaa ttgttccgct tgcttcagcc
    39001 agtaaatcgg caaggtgatg aatataatcc gcccggcccg gtactggtgg gcagagaaaa
    39061 ccgtcgggga tatcccagtt cgctacggcg taaaaatggg ccagcaacgc cttattgagc
    39121 gccttcaccg ccagcggatt ggcaaagtct acgctttgct cccctgcggg tgtaagcgtg
    39181 aggaattgcc tgagttcagg attgacctga caaagcgtgg cgagatcgta gcggctgtga
    39241 tgacggttgc gcggatgcaa ccccggtttc tgggcggaca tagcattctc ctttaagcag
    39301 cggcgtaaga tacccgcata acgcgacagg gtaaatatct ggctgcgttt atcattcccc
    39361 aaaaccttaa catggctatg gtttatggca attcatcata aaaatcagca ttatgcatgt
    39421 cattgatttg tattattttg taaaaatata tcgttcaaaa aataagcata tctctgtcaa
    39481 ttttttgcgt catttgcatc gcgcaggcga cctacactta tcaggcacta cccgttgtat
    39541 ttatgaggat gtcattatga aaaagtgcct cacactactg attgccaccg tcctgagcgg
    39601 aatctctctc acggcttatg ccgcgcaacc gatgagtaac ctggacagcg gtcagctacg
    39661 gcccgccggt accgtttcgg caactggcgc atcaaaccta agcgatctgg aggataaact
    39721 ggcagaaaaa gcgcgcgaac aaggcgcgaa aggttatgtc attaattccg caggcggaaa
    39781 tgaccagatg ttcggtactg caaccatcta caaataaccc caccaacgta actgcattcg
    39841 cctctggatg cagttttcgc ctgttttgta atcaaaaaac atcaaatatg aacgcaatgt
    39901 aaataaatgt atttcttttt cgcgcaatgg gtgatagaaa atcgcgtcaa gtgataatgc
    39961 ttatcaaaat tattatcact tgcacaaaca ctatcacggg attaacagtg gcatcgcatc
    40021 cgcagagatg ctttctcgtg gcagtgaaaa tttcaacata taagaaaaag tcacctgcaa
    40081 aatggaaaac aatcgcaatt tccctgccag acaatttcat tcgctcacgt tctttgccgg
    40141 tctttgtatt ggtattacgc ctgtggcaca ggcactcgcc gccgaagggc aagctaacgc
    40201 ggatgacacg ctggttgtcg aagcatcaac gccttcgctt tatgcgccac
//
LOCUS       DAJTYG010000025        39558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTYG010000025 DAJTYG010000000
VERSION     DAJTYG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.025"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..188)
                     /locus_tag="OO568_001446"
     CDS             complement(<1..188)
                     /locus_tag="OO568_001446"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR6640854.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKG"
     gene            complement(329..2362)
                     /gene="metG"
                     /locus_tag="OO568_001447"
     CDS             complement(329..2362)
                     /gene="metG"
                     /locus_tag="OO568_001447"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6640855.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDKYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2494..3603
                     /gene="apbC"
                     /locus_tag="OO568_001448"
     CDS             2494..3603
                     /gene="apbC"
                     /locus_tag="OO568_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6640856.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3865..4146
                     /locus_tag="OO568_001449"
     CDS             3865..4146
                     /locus_tag="OO568_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR6640857.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4439..4981
                     /locus_tag="OO568_001450"
     CDS             4439..4981
                     /locus_tag="OO568_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6640858.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5062..5736
                     /locus_tag="OO568_001451"
     CDS             5062..5736
                     /locus_tag="OO568_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6640859.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5752..8232
                     /locus_tag="OO568_001452"
     CDS             5752..8232
                     /locus_tag="OO568_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6640860.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPLVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            8248..9281
                     /locus_tag="OO568_001453"
                     /pseudo
     CDS             8248..9281
                     /locus_tag="OO568_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9363..9701)
                     /gene="rcnB"
                     /locus_tag="OO568_001454"
     CDS             complement(9363..9701)
                     /gene="rcnB"
                     /locus_tag="OO568_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6640861.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9920..10756)
                     /gene="rcnA"
                     /locus_tag="OO568_001455"
     CDS             complement(9920..10756)
                     /gene="rcnA"
                     /locus_tag="OO568_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCR6640862.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTV
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHDHEHHQDHEHHHDQGHHHHHEHGEYQDA
                     HANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFS
                     IGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGI
                     MR"
     gene            10877..11149
                     /gene="rcnR"
                     /locus_tag="OO568_001456"
     CDS             10877..11149
                     /gene="rcnR"
                     /locus_tag="OO568_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR6640863.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11241..11337
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11372..12160
                     /gene="thiM"
                     /locus_tag="OO568_001457"
     CDS             11372..12160
                     /gene="thiM"
                     /locus_tag="OO568_001457"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6640864.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12157..12957
                     /gene="thiD"
                     /locus_tag="OO568_001458"
     CDS             12157..12957
                     /gene="thiD"
                     /locus_tag="OO568_001458"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6640865.1"
                     /translation="MKRINALTIAGTDPSGGAGILADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13022..13840
                     /locus_tag="OO568_001459"
     CDS             13022..13840
                     /locus_tag="OO568_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6640866.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            13892..14652
                     /locus_tag="OO568_001460"
                     /pseudo
     CDS             13892..14652
                     /locus_tag="OO568_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(14626..15591)
                     /locus_tag="OO568_001461"
     CDS             complement(14626..15591)
                     /locus_tag="OO568_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6640867.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIRAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15588..16591)
                     /locus_tag="OO568_001462"
                     /pseudo
     CDS             complement(15588..16591)
                     /locus_tag="OO568_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16588..17064)
                     /locus_tag="OO568_001463"
     CDS             complement(16588..17064)
                     /locus_tag="OO568_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6640868.1"
                     /translation="MLARSSFHRKGIQKVLLCGIVIAYLTLPVNFTYALLFLGILLHG
                     VSYDFYYVTAYIYVDKKAPVHMRTAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQE
                     PVNGLTFNWSGMWTFGAVMIAIIAVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            17218..18270
                     /gene="fbaB"
                     /locus_tag="OO568_001464"
     CDS             17218..18270
                     /gene="fbaB"
                     /locus_tag="OO568_001464"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6640869.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVAVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            18578..19432
                     /gene="gatY"
                     /locus_tag="OO568_001465"
     CDS             18578..19432
                     /gene="gatY"
                     /locus_tag="OO568_001465"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6640870.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRLKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNLAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            19461..20723
                     /gene="gatZ"
                     /locus_tag="OO568_001466"
     CDS             19461..20723
                     /gene="gatZ"
                     /locus_tag="OO568_001466"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR6640871.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAVVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            20733..21185
                     /gene="gatA"
                     /locus_tag="OO568_001467"
     CDS             20733..21185
                     /gene="gatA"
                     /locus_tag="OO568_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6640872.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            21216..21500
                     /gene="gatB"
                     /locus_tag="OO568_001468"
     CDS             21216..21500
                     /gene="gatB"
                     /locus_tag="OO568_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6640873.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            21504..22860
                     /locus_tag="OO568_001469"
                     /pseudo
     CDS             21504..22860
                     /locus_tag="OO568_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            22908..23948
                     /gene="gatD"
                     /locus_tag="OO568_001470"
     CDS             22908..23948
                     /gene="gatD"
                     /locus_tag="OO568_001470"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6640874.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24054..24827
                     /locus_tag="OO568_001471"
     CDS             24054..24827
                     /locus_tag="OO568_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6640875.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(24909..25808)
                     /gene="yegS"
                     /locus_tag="OO568_001472"
     CDS             complement(24909..25808)
                     /gene="yegS"
                     /locus_tag="OO568_001472"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6640876.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            26214..26531
                     /locus_tag="OO568_001473"
     CDS             26214..26531
                     /locus_tag="OO568_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640877.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(26873..28234)
                     /gene="yegQ"
                     /locus_tag="OO568_001474"
     CDS             complement(26873..28234)
                     /gene="yegQ"
                     /locus_tag="OO568_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6640878.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEVMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(28381..28703)
                     /locus_tag="OO568_001475"
                     /pseudo
     CDS             complement(28381..28703)
                     /locus_tag="OO568_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
     gene            complement(28894..29616)
                     /gene="baeR"
                     /locus_tag="OO568_001476"
     CDS             complement(28894..29616)
                     /gene="baeR"
                     /locus_tag="OO568_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6640879.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHESGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(29613..31016)
                     /gene="baeS"
                     /locus_tag="OO568_001477"
     CDS             complement(29613..31016)
                     /gene="baeS"
                     /locus_tag="OO568_001477"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6640880.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLIDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(31013..32428)
                     /locus_tag="OO568_001478"
     CDS             complement(31013..32428)
                     /locus_tag="OO568_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6640881.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(32429..35494)
                     /gene="mdtC"
                     /locus_tag="OO568_001479"
     CDS             complement(32429..35494)
                     /gene="mdtC"
                     /locus_tag="OO568_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6640882.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLRITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(35495..38617)
                     /gene="mdtB"
                     /locus_tag="OO568_001480"
     CDS             complement(35495..38617)
                     /gene="mdtB"
                     /locus_tag="OO568_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR6640883.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPSASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKSLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLVLWTK
                     SRFARHEEEA"
     gene            complement(38617..>39558)
                     /locus_tag="OO568_001481"
     CDS             complement(38617..>39558)
                     /locus_tag="OO568_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HCR6640884.1"
                     /translation="TGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
                     SQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGT
                     IKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDL
                     VFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKA
                     RFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLV
                     TPGIQDSQKVEVVEAQSATTPEEKATSREYAKKGARS"
BASE COUNT         9919 a         9688 c        10239 g         9712 t
ORIGIN      
        1 cccttcttca ccttctccgc aatcagcttc agttccgctt tttccgccgc cgcagcatct
       61 gaaaaacttt ttatctgact ttgccctttg gtgccaactt ttccccaact gatatgcaac
      121 tcgtttccct gttgctccac cgtccagaat ttatgtgatt tttcgtcctg atagataaag
      181 tgtctcattc tgcatccctg taatttcatc cagttgaatg cagatgctac cagtatttat
      241 acgggttaga gagagacaaa atcgcaagaa aacaacttta ggaatttata aagaaaaggc
      301 gctgtcgatg cagcgccttg aagggggatt atttcacctg atgacccggt ttagcaccgg
      361 catccgggct tagcaggaaa atatctttcc cgccaggacc ggcagccatc accatgcctt
      421 cagagatacc gaagcgcatt ttacgtggtg ccaggttagc caccataatg gtgtgacgac
      481 caatcagtgc ctgcggatcc gggtaagcag aacgaatacc ggagaagaca ttgcgttttt
      541 caccgccgag atccagcgtc aggcgcagca gtttgtcaga accttcaaca aactctgcgt
      601 tttcgatcag cgccacgcgc aggtcaactt tggcgaagtc gtcaaaggtg atggtttcct
      661 gaatcggatc atctgccagt gggccagtaa ctggcgcggt agtggctttc acttcttctt
      721 tagaggcttc caccagagct tcaacctgtt tcatatcgat gcggttatac agcgccttga
      781 acggattcac tttgtggccc agcagcggtt gctggatacc atcccaggtc agttccgtat
      841 tgaggaaggc ttctgcacgc tcggtcagtt tcggcagtac cggcttcagg taagtcatca
      901 gcacgcggaa caggttgatg cccattgagc aaatcgcctg caggtcagca tcgcgacctt
      961 cctgttttgc gactacccac ggagcctgct cgtcgacata gcggttagcc aggtcagcca
     1021 gtgccatgat ttcgcgcact gctttaccaa attcacggct ttcccacgct tcaccaatca
     1081 cttcagcggc atcggtaaag gttttgtaca gctcagggtc agccagctcg cttgccagca
     1141 caccgtcaaa acgcttgttg ataaagcccg cattacggga ggccaggtta accactttgt
     1201 tcacgatatc ggcattcaca cgctgaacga aatcttccag gttgagatcg atatcatcaa
     1261 tgcgcgaaga gagtttcgca gtgtagtagt aacgcaggct gtcagcgtca aaatgattca
     1321 gccaggtgct ggctttaata aaggtgccgc gagacttgga catcttcgcg ccgttcaccg
     1381 tcacatagcc atgaacaaac aggttggtcg gcttgcggaa gttgctgcct tccagcatgg
     1441 caggccagaa caggctgtgg aagtaaacga tatctttacc gatgaagtgg tacagctcgg
     1501 cggtggagtc tttcttccag tatttatcga agcttacgct gtcgccgcgc ttgtcgcaca
     1561 gattcttgaa agaacccatg tagccaatcg gtgcgtccag ccagacgtag aaatatttgc
     1621 ccggcgcgtt cggaatttca aaaccaaagt atggcgcgtc gcgggagata tcccactgtt
     1681 gcaggccgga ttcaaaccac tcctgcattt tgtttgccac ctgctcctgc aacgcaccgc
     1741 tgcgggtcca tgcctgcagc atttcgctga aagagggcag atcaaagaag aagtgttcag
     1801 aatcacgcat cactggtgta gcaccagaaa ccaccgattt cggctcgatc aattcagtcg
     1861 ggctgtaggt cgcgccgcag acttcgcagt tatcgccgta ttgatccggg gatttacatt
     1921 tcgggcaggt gcctttcaca aaacggtccg gcaggaacat gcctttttcc ggatcgtaca
     1981 gctgagagat ggtgcggttt ttaataaaac cgttttcttt caggcgagag tagataagtt
     2041 ctgacaactg gcggttctct tcgctgtgcg tcgagtgata gttgtcatag ctgatgttaa
     2101 agcctgcgaa atcagtctga tgctcctgac tcatttcgcc aatcatctgc tccggggtga
     2161 taccgagctg ctgagctttc agcatgatcg gtgtaccgtg ggcatcgtcg gcgcagatga
     2221 agttgacctc gtggccgcgc attcgctggt aacggaccca gacatcagcc tggatgtgct
     2281 ccagcatatg gccgaggtgg attgagccgt tagcgtacgg cagtgcgcac gtcaccagaa
     2341 ttttcttcgc gacttgagtc atagtaggca ttacttctta atagtgaaaa ggggttagat
     2401 agtaccaaat gggaaaatgt taagtaagta cttctctata ttcttcaggt aaactatcag
     2461 ctgatggttt atctatacaa aaaaggagtc gggatgaacg aacaatccca ggccaaatca
     2521 ccagaagcct tgcgcgcaat ggtcgccggg acactggcca attttcagca cccaaccctg
     2581 aagcataacc tcaccacgct gaaagcgttg caccatgtcg cctggatgga cgacacgttg
     2641 catgtggaac tggttatgcc gtttgtctgg catagcgcgt tcgaagagtt aaaagagcaa
     2701 tgtagcgccg aactgctgcg catcaccggg gcgaaagcta tcgactggaa actgtcgcat
     2761 aacatagcca ctctgaaacg cgtgaagaat cagccgggaa ttaacggtgt gaagaacatt
     2821 atcgcggtga gttccggtaa aggcggcgtg ggtaaatcgt ccacggcggt aaacctggcg
     2881 ctggcactgg cggcggaagg ggcgaaagtc ggcattctgg acgccgatat ctacggccca
     2941 tcaattccca ccatgctggg cgcagaaaat cagcgtccaa cttcgccgga cggtacccac
     3001 atggcaccga ttatgtctca tggcctggca accaattcaa ttggttatct ggtcactgac
     3061 gacaatgcga tggtgtggcg tggaccgatg gcgagcaagg cgttaatgca gatgttgcag
     3121 gaaaccttgt ggccggatct cgactatctg gtgcttgata tgccgccagg caccggtgac
     3181 attcaattga cgctggcgca aaacattcca gtaactggtg cagtcgtggt caccaccccg
     3241 caggacatcg cgttgatcga tgcgaaaaaa ggcattgtga tgttcgagaa agtcgaggtt
     3301 ccggtgctcg gtattgtcga gaacatgagt gtgcatattt gcagtaactg tggtcaccat
     3361 gaaccgattt tcggtaccgg tggtgcagag aaactggctg aaaaatacca tacacagctg
     3421 ctggggcaga tgccgttaca catctcctta cgtgaggatc tcgataaagg cacgccaacg
     3481 gttatcagcc gtccggagag cgaatttacc gctatctacc gccagctggc tgaccgcgtt
     3541 gcagctcagc tctactggca gggtgaagtc attcctggcg agatttcctt ccgcgcggtc
     3601 taacgcctat taaccattct ggttattctt catacaccgc agagatgtta tattgatgcg
     3661 gtgtatttgt ttttgccgca ttaatgaaca cttatttaat actgcgaata ttctgcggac
     3721 cttcgtccga aactctacaa agtcgagtga tcgtacaggc aatgcttcat tatcatcctt
     3781 cgtgctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat ccaggcataa
     3841 cctttactac agggataacg ttcaatgaat aagtattggc tgtcaggaat tatttttctt
     3901 gcctatggat tggcttctcc cgcagtctct tcagagactg ccacattaac gattaatggc
     3961 aggatcagtc ccccaacatg cagtatggct atggttaata gtcaaccaca acagcattgt
     4021 ggtcagttaa cgtataatgt tgacacccgt catcaggttt cttcgcccgt caaaggtgtt
     4081 acgacagaag tggttgttgc aggtagcgat agcaaacgtc gaatcgtgtt aaatcgctac
     4141 gattaatttg gcaacctgat tttaattgca tttagaaaga cgaaatcatt tatataaatg
     4201 atttcgtctt ttttattgat atcaacaata ccatttacat aaatctacat taattaatag
     4261 atgtgataaa tatttaatat tcttaagtat ttgttgtatt tatattgcat tggttaattt
     4321 gttttaaatc aataaaacaa tatgtttaca ttgcggcagc ttttttgatg tgagaaattt
     4381 gtttccaaga gatacagact cttaacaacc aaaaataaat tttattaagg aacatattat
     4441 gaaacgttca attattgctg ccgctgtctt ttcttctttt tttatgagcg ctggagtatt
     4501 tgctgcagac gttgataccg gaacattaac cattaaagga aatattgcag aatctccgtg
     4561 taaattcgaa gcgggtggtg attcagtaag tattaatatg ccgactgtac caaccactgt
     4621 ctttgaaggt aaagctaaat attctaccta tgatgatgca gtcggtgtaa ccagtagcat
     4681 gttaaaaatt agctgcccga aagaagttgc tggtgtaaaa ctctcgttga tcacaaacga
     4741 caaaataacc ggtaacgata aggcgatagc cagtagcaac gataccgtgg gttactatct
     4801 ctatttaggt gataacagcg atgtcctaga tgtttctgca ccttttaaca ttgagagtta
     4861 taaaacagca gaaggtcaat atgctattcc gtttaaagca aaatacctga aactgacaga
     4921 taactcagtg caatcaggtg atgtgttatc ttctctggtt atgcgtgtag cgcaggatta
     4981 ataaataaaa ttgcaagttt ctccaggtaa taaacatggc cgctatcccc atggcggctt
     5041 tttaatttaa gaggcattaa gatgaaagga ttattatctt tactcatttt ttctatggtc
     5101 cttcctgcac atgccggaat tgttatctac gggacgcgca ttatttaccc ggcagaaaat
     5161 aaagaagtta tggtgcagtt gatgaaccag ggaaatcgtt cttcgctgct gcaggcgtgg
     5221 attgatgatg gcgatacgtc gttaccacca gaaaaaattc aggttccttt catgttaacg
     5281 ccaccagtgg caaaaatagg ggcaaattcc gggcagcaag taaaaatcaa aattatgccg
     5341 aataaactgc ccactaataa agaaagcatt ttttatctga atgttctgga tattccacca
     5401 aatagtccag agcaagaagg caagaatgca ctgaagtttg cgatgcaaaa cagaattaag
     5461 ttgttttacc ggccagcggg tattgctccg gtaaataaag cgacctttaa aaagttactg
     5521 gtaaatcgca gtggcaatgg attggtgata aaaaatgact cagctaattg ggtgacgatt
     5581 tcggatgtca aagctaataa tgttaaagtc aattatgaaa ctattatgat tgccccctta
     5641 gaaagtcaga gtgttaatat caaaagtaat aatgcaaata actggtatct gaccattatc
     5701 aatgaccacg gcaactatat tagtgacaaa atttaattgc aggagctgcc tatgttgaga
     5761 atgaccccac ttgcatcagc aatcgtagcg ttattgctcg gcattgaagc ttatgcagct
     5821 gaagaaacct ttgataccca ttttatgata ggtggaatga aagaccagca ggttgcaaat
     5881 attcgtcttg atgataatca acccttaccg gggcagtatg acatcgatat ttatgtcaat
     5941 aagcaatggc gcgggaaata tgagattatt gttaaagaca acccgcaaga aacatgttta
     6001 tcaagagaag ttatcaagcg gttaggcatt aatagcgata acttcgccag cggtaagcaa
     6061 tgtttaacat ttgagcaact tgttcagggt gggagctata gctgggatat cggggttttt
     6121 cgtctcgatt tcagtgtccc gcaggcttgg gtggaagaac tggaaagtgg ctatgttcca
     6181 ccggaaaact gggagcgggg tattaatgcg ttttatactt cttattatgt gagtcagtat
     6241 tacagcgact ataaagcgtc gggtaacaac aagagtacat atgtacgttt taacagcggg
     6301 ttaaatttac tggagtggca actgcattct gatgccagtt tcagtaaaac aaataacaat
     6361 ccaggggtgt ggaaaagcaa taccctgtat ctggaacgtg gatttgccca atttctcggc
     6421 acgcttcgcg tgggtgatat gtacacatca agcgatattt ttgattctgt tcgcttcagc
     6481 ggtgtgcggt tgtttcgtga tatgcagatg ttgcctaact cgaaacaaaa ttttacgcca
     6541 cgggtgcagg ggattgctca gagtaacgcg ctggtaacta ttgaacagaa tggttttgtg
     6601 gtttatcaga aagaggttcc tcctggcccg tttgcgatta cagatttgca gttggccggt
     6661 ggtggagcag atcttgatgt tagcgtgaaa gaggcggacg gctcggtaac cacctatctg
     6721 gtgccttatg cagcggtgcc aaatatgctg caacccggcg tgtcgaaata tgattttgcg
     6781 gcgggccgta gccatattga aggggcgagc aagcaaagtg attttgtcca ggcaggttat
     6841 cagtatggtt ttaataactt attgacgctg tatggcggca cgatggttgc taataattac
     6901 tatgcgttta ccctcggaac gggttggaac acgcgcattg gcgcaatttc cgtcgatgcc
     6961 acgaagtcgc atagtaaaca agacaacggc gatgtgtttg acgggcaaag ttatcaaatt
     7021 gcctacaaca aatttgtgag ccaaacgtcg acgcgttttg gtctggcggc ctggcgttat
     7081 tcgtcgcgtg attaccggac atttaacgat cacgtttggg caaacaataa agataattat
     7141 cgccgtgatg aaaacgatat ctatgacatt gccgattatt accagaacga ttttggccgt
     7201 aaaaatagct tttctgccaa tatgagccag tcattgccag aaggctgggg ttctgtgtcc
     7261 ttaagtacgt tatggcgaga ttactggggg cgtagcggca gcagtaagga ttatcagttg
     7321 agttattcca ataactggcg gcggataagc tataccctcg cggcaagcca ggcgtatggc
     7381 gagaatcatc atgaagagaa acgttttaat atttttatat caattccctg tgattggggt
     7441 gatgacgtta cgacgcctcg tcggcaaata tatatgtcta actcaacgac gtttgatgat
     7501 cagggttttg cctcaaataa tacgggatta tcaggaacag tagggagtcg ggatcagttc
     7561 aattatggtg tcaacctgag tcatcaacat cagggaaatg aaacgacagc tggggcgaat
     7621 ttgacctgga acgcgccggt tgcgacagtg aatggcagtt atagtcagtc gagtacttat
     7681 cgacagactg gagccagtgt ttcagggggc attgtcgcct ggtcgggtgg cgttaatctg
     7741 gcgaaccgtc tttccgaaac gtttgctgtg atgaatgcgc caggaattaa agatgcttat
     7801 gtcaatgggc aaaaatatcg cacaacaaac cgtaatggag tggtggtata cgacggaatg
     7861 acaccttatc gggaaaatca cctgatgctg gatgtgtcgc aaagcgatag cgaagcagaa
     7921 ttacgtggca accggaaaat tgccgcccct tatcgcggcg cggttgtact ggttaatttt
     7981 gataccgatc agcgcaagcc ctggtttata aaagcgttaa gagcggatgg tcaaccatta
     8041 acgtttggtt atgaagtcaa tgatatccat ggtcataata ttggcgttgt cggccaggga
     8101 agccagttat ttattcgcac caatgaaata ccgccattgg ttaatgtagc aattgataag
     8161 caacaaggac tttcatgcac aatcaccttc ggtaaagaga ttgatgaaag tagaaattat
     8221 atttgccagt aatagtcagg tggttctatg gaaattcgca taatgctatt tatattaatg
     8281 atgatggtta tgcctgtgag ctatgcggca tgttatagtg agttatctgt tcagcacaat
     8341 ttggttgttc aggggaattt tgcacttact caaacacaaa tagcgacata tgagcataat
     8401 tttaatgatt cgtcatgcgt gagtacaaat actatcaccc ctatgagccc atcggatatt
     8461 attgttggac tttataacga taccattaaa ttaaatttac attttgaatg gaccaataaa
     8521 aacaacatca cgttgtcaaa taatcagagc agtttcacca gtggttattc ggtaacggtg
     8581 acacctgcgg ccagtaatgc aaaagtgaat atttctgcgg ggggcggtgg ttcagtgatg
     8641 attaatggtg ttgcgacatt atccagtgct tcatcatcga cacgcgggag tgccgcagta
     8701 caatttctac tgtgtttatt aggtggcaag tcatgggatg catgtgtaaa tagctacaga
     8761 aatgcattgg cacaaaatgc aggtgtctat tccttcaatc tgacattgtc atacaacccg
     8821 ataaccacaa cctgcaaaca ggacgattta ttaattactt tagacagtat tcccgtttca
     8881 caattaccag ccacaggtaa caaagcaaca ataaatagta aaaaaaggga tattattctg
     8941 cgttgtaaaa atttattagg tcaacaaaat caaacatcac ggaaaatgca ggtgtattta
     9001 tcaagttctg acttgttaac caacagcgac acaatactga aaggtgcgga agataatgcg
     9061 taggatttat tcttgaaagt aatggttcgc cagtcacact tttaaatatc actaacagca
     9121 gtaaaggata cacaaattta aaagaaattg cggcaaagtc aaaacttaca gatacaacgg
     9181 tttcaattcc gataacagcc agttactacg tctacgatac aaacaaagtt aaatctggcg
     9241 cactggaggc aaccgcatta atcaacgtga aatacgacta ataacaagca agacgagcaa
     9301 gtggctaata ataaaaaata acaaggtgtc tgcatttccc cctcatgatg agggggcttt
     9361 ttttagcgat gataaaaaat ctcaccgtcg taggctttaa tgattttacc gtcggtgtcg
     9421 ctgatcaaca cgtacgcgcc acccatatag gtccagtgcg tcccggcatc aggcgcgggc
     9481 aggttacgta actgccactg cttaatgttg tactcatcgg tgcggtacat ctctgggacg
     9541 gtatcacctg gtttgaaatg cttaaagtca gcaatgaaac tacttaactc atacttagaa
     9601 attcccgagg tattggtcga acccgcggtg gctggtgcgg cccaggcggc actggtaacc
     9661 agcaaaagca cacccagcaa catcttattt ttaatggtca taaactctcc actttgggga
     9721 tctgtaatat cagtcgcata tctttcctgc catacaatat aactgacaag cgatattttg
     9781 ttagtgatcc catgatttcg taaaaagtgt aaatgaaagg atgccctaaa agcatcctta
     9841 gcgagcagag agtcagcgga aatagctgat ttaagaatca tcctgggctg aagataacat
     9901 gcaagcctaa aggattgagt tatcgcatta tgcccatgaa gccatgtacg cccatataca
     9961 caccgactaa gccaatcaac agactggaaa aatagggggc gcgtttagcg agagtgttaa
    10021 atccgctcca gcgttttgcg acctgctgaa cgctgattgc tgcgccaacg cctacggtga
    10081 caagcgttaa cgccaggcca atgctgaaac tgacgaccag tgttgcgccc agtgtcaggg
    10141 ctttcaactg aatgcatatc aacagcacgg taattgctgc cgggcagggg ataaggccac
    10201 cggttaagcc aaataacaaa atttgccagt tgatgacctc tctaccatca aagcgtcgtt
    10261 taatgtcatt ggcatgggca tcctgatact cgccatgttc gtgatggtga tgatgtcctt
    10321 ggtcgtggtg atgttcgtgg tcttgatgat gttcgtgatc gtgatgatgg tgatgatgct
    10381 cataatcatg cccgtgcata ttctccagcc agttgcgttc gccgcgccag gtacgccaga
    10441 acatccagaa cacggtgcca ataatgatca ctgcggaaat cagctggagc cacggttctg
    10501 ctgattgagc agtaaagcgc ttactgatca ccatcccgcc aaaggcaatc aaccagacca
    10561 ccgcggtatg cgaaatggtt gctgccagtc cgagcatcac cgcctgttta atggtgcctt
    10621 tgatggcgat gataaacgcc gccatcatcg tttttgagtg ccctggttcc aggccatgaa
    10681 gcgcaccaag taagatggcg ctggggatga agaaccaggc gtttccttgc tgaagaagag
    10741 ttgtaaattc ggtcatgata ataattctta gtattaattc ggcaatctga ttctactccc
    10801 ccccagtacc tgatactacc ccccagtaga ttaatagtgc tatgattttt catattcttg
    10861 ttaaccaggt gttgccatgt ctcatacaat ccgtgataaa cagaaactga aagcgcgtgc
    10921 cagtaagatt cagggccagg tcgtggcgct caagaaaatg ctcgacgagc cgcacgaatg
    10981 cgctgcagtt ttacaacaga ttgctgctat ccgtggagcg gtaaacggtc tgatgcggga
    11041 agtgattaaa ggtcatctga cggaacacat cgttcaccag ggggatgagc taaaacgtga
    11101 agaagatctg gatgtcgttc tgaaggtgct ggattcatat atcaaataat ttattaacgc
    11161 gattgtaaaa ctgccgtttt tcctcgttta caacgggtgc gctggacatt accatcctcc
    11221 tctgcgattt atcatcgcaa ccaaacgact cggggtgccc ttctgcgtga aggctgagaa
    11281 atacccgtat cacctgatct ggataatgcc agcgtaggga agtcacggac caccaggtca
    11341 ttgcttcttc acgttatggc aggagcaaac tatgcaagtc gacctgctga gttcagcgca
    11401 atctgcgcac gcgttacacc tttttcacca acattcccct cttgtgcact gcatgaccaa
    11461 tgatgtggtg caaaccttta ccgccaatac cttgctggcg ctcggtgcat cgccagcgat
    11521 ggttatcgaa accgaagagg ccagtcagtt tgcggctatc gccagtgcct tgttgattaa
    11581 cgttggcaca ctgacgcagc cacgcgcaca ggcgatgcgt gctgccgttg agcaagcaaa
    11641 aagctctcaa acacgctgga cgcttgatcc agtagcggtt ggtgcgctcg attatcgccg
    11701 ccatttttgt catgaacttt tatcttttaa accggcagcg atacgtggta atgcttcgga
    11761 aatcatggca ttagctggca ttgctaatgg cggacgggga gtggatacca ctgacgccgc
    11821 agctaacgcg atacccgctg cacaaacact ggcacgggaa actggcgcaa tcgtcgtggt
    11881 cactggcgag gtggattatg ttaccgatgg acatcgtatc attggtattc acggtggcga
    11941 tccgttaatg accaaagtgg taggaactgg ctgtgcatta tcggcggttg tcgctgcctg
    12001 ctgtgcgtta ccaggcgata cgctggaaaa tgtcgcatct gcctgtcact ggatgaaaca
    12061 agccggagaa cgcgcagtcg ccagaagcga ggggccaggc agttttgttc cacatttcct
    12121 tgatgcgctc tggcaattga cgcaggaggt gcaggcatga aacgaattaa cgctctgacg
    12181 attgccggta ctgatccgag tggtggtgcg gggattctgg ccgatcttaa aaccttctcg
    12241 gcacttggcg cttatggttg ctcagttatt actgcactgg tggcgcaaaa tacccgtggc
    12301 gtacagtcgg tgtatcgcat tgagcctgat tttgtcgccg cccagctcga ttcggtgttc
    12361 agcgatgtgc gaatcgatac cactaaaatc ggtatgttgg cggaaaccga tattgttgaa
    12421 gcggtggcag aacggttgca acgttatcag atccaaaacg tggtactcga caccgttatg
    12481 ctggcaaaaa gcggcgaccc gctgctttca ccttcggcgg ttgctacgct gcgcagtcga
    12541 ttattgccac aggtttcatt aataacgcca aacttgcccg aagctgccgc cttgctcgac
    12601 gcgccacacg cgcgcaccga acaggaaatg ctggaacaag ggcgatcgct gttggcgatg
    12661 ggctgtggcg cagtgctaat gaaaggtggt catctggatg atgagcaaag cccggactgg
    12721 ctgtttaccc gcgagggtga acaacggttt accgcaccgc gcattatgac caaaaacacc
    12781 cacggcactg gttgtacact ctctgcggcg ttggctgcac tacgcccgcg ccatacaaac
    12841 tgggctgaca ccgtacagga ggcaaaaagc tggctttcat cggcgttagc ccaggccgac
    12901 acgctggaag ttggtcacgg tattggtccg gttcaccact tccacgcctg gtggtgagca
    12961 ccgtactata gctgctttgg ttattgtcag cctttggcac tgacattcag ggatgaaacc
    13021 aatgcagcta agaattacca gtcgaaaaaa actcaccgct ctattatgcg ctcttgggct
    13081 aatttccatc gttgcaattt atccgcgtca gacggtgaat tttttctatt cgacagcagt
    13141 tcagattaca gactacatcc acttctacgg ttatcgcccg gttaaatctt tcgctattcg
    13201 cattcctgcc agttacacca ttcacggatt agatgtttcg cgctggcagg agcgtatcga
    13261 ctggcagcgg gtggcaaaaa tgcgcgacaa cgatatccgc ttacagtttg cttttattaa
    13321 ggctacggaa ggcgaaaagc tggtggaccc ctatttttcg cgtaactggc aattaagccg
    13381 cgaaaatggc ctgctgcgcg gggcgtatca ttatttctcc ccgtcggtat ccgcttcagt
    13441 tcaggcgcga ttatttctgc aaacggtgga tttctcacaa ggcgatttcc ctgccgtgct
    13501 ggacgtagaa gaacggggaa aattatcggc aaaagaatta cgcaagcggg taagtcagtg
    13561 gctaaaaatg gtcgaaaaac gcacgggaaa aaagccgatt atttactcag gagccgtttt
    13621 ttatcacacc aatctggcgg gctatttcaa tgaatatccg tggtgggtgg ctcactatta
    13681 tcaacgtcgt ccggacaatg acggcatggc ctggcgcttc tggcagcatt ccgaccgtgg
    13741 acaggtagat ggcatcaatg gtccggtgga ttttaatgtg tttaatggca cggtggatga
    13801 gttgcaggca ttcgttgatg ggattaaaga aacgccttaa ataagaagca gtgatatact
    13861 cccaccagac tccctggcag gaaaaagaca aatggaacaa gcgcataccc agctgattgc
    13921 ccaactgaac gagcgtattt tagcggcgga taacacgccg ctttatatta agtttgccga
    13981 aacggtaaaa aatgccgtgc gcagcggggt gctggagcat ggcaatattt tgcccggtga
    14041 gcgtgattta agtcagttaa ccggcgtttc gcgcattacg gtgcgcaagg cgatgcaggc
    14101 gctggaagaa gagggcgtgg tgacgcgttc gcgcggttac ggcacgcaga tcaacaacat
    14161 cttcgaatac tcgttgaaag aagcgagagg tttttctcag caggtggtat tgcgcgggaa
    14221 aaagcccgat acgctatggg ttaataagcg tgtcgtgaaa tgtcccgaag aagttgcgca
    14281 gcagctggcg gtcgaagcgg gaagtgatgt ctttttgctt aagcgtattc gctatgtcga
    14341 tgaagaagcg gtatcgattg aggaatcgtg ggttccggcg catttaattc atgatgttga
    14401 tgccatcgga atttctcttt atgactattt ccgcagccag cacatttacc cacaacgtac
    14461 gcgttcccgc gttagcgccc ggatgccgga tgccggatgc cggatgccga gtttcagtca
    14521 catattcagt tagatagcaa aataccggtg ctggtgatca agcaagtggc gcttgaccaa
    14581 cagcaacggc ctattgagta cagcattagc cactgtcgca gcgatctata cgtttttgtg
    14641 tgcgaggagt agttcctcgc gcgttggcgc acaatcaccg ccccgatgcc cgacaaccca
    14701 cgacgccact gcattgccca gtaatacggc atccgccagt ggcagaccag aggccagccc
    14761 ggcaagtacg ccacctgcat gactgtcgcc cgccccaatg gtgtctacaa cttgcgttgg
    14821 aaatgcccga atgcagccag aagcgtcgtt gctgaaatac catgcgcctt ctttatcgag
    14881 acgaacgatc aacggcgcgg caaatttctc ctgccattgc ttgccaagtg ttgttatctc
    14941 tgcagataaa gcaaaacgtt cggcggcaat ctcagcctct tgacgattga gtgacactaa
    15001 aggtcgacag gccatgatcc gcgccagtaa tgcgtccggg atatcgccaa tacgtgggcc
    15061 aaaatcgata aacggcgtca cgtcttgcag cttttccagc cattccacta acaattcgcc
    15121 gcagggcgag gccagttgat aaccggaaaa atagagcagg ttgccaggcg caacggttaa
    15181 tcgtgccaac cattggcgat tccattgatt ttccacaccg ctgaatgaca taaaagtgcg
    15241 ttcgccatcc ggctcaacca gtgccagaca ccagccgtta tcgctttcgg cgttatcgat
    15301 caggctgatt aacccctctt tcgccatccg gttgcgaata atctccgccc acacgccctg
    15361 accgagcggc aaggcattac ccgcttcgat gccgaggcgc tttaacgcca cggcaatatt
    15421 cagtgcgcag ccgccaacgt taacgctctg ctgtttcagt tcgatatcgc acccacgcca
    15481 ggggagggca taagcgtcgg cgatcacgtc aatgaccgcc gcgccgacga ccatcaccgg
    15541 ctgacgcgtg gttaattcag gcagcagtgt gtgtaatcga gcgccgctca taccgcctcc
    15601 cgttgttgac gatatttcgc cagcgctgtg gcatagcggt tgaaatcaag ctgatttacc
    15661 gcatccagct ccgcctttaa cgcaggatcg acagcgttaa tgccatgcaa cgcgccgcaa
    15721 atcgccgtcg ccatagcacc tatggtgtct gtgtcgccgc caagattggc gcacaggacg
    15781 gcgcagcggt ttgggtcggt ttgtgccagt tcaactagcg caatggcgca cggaacggac
    15841 tcaatagtgc tggtgcctgc gccaacgacc tggtaaagct gttcgctggc ggattcggtg
    15901 ccgtcggcat tacgcacaat tttcagcgca atctccagac gtgccgccag tgaggcgctg
    15961 aacgtggtgg tgcgtttttg ctgtgcatgt cgcgcaattg aaggcaatga atcaacaatc
    16021 gctgaccagc gttctccgtc aatggcacga gaaatcgccc atgcgatgac taccgcgcct
    16081 gcaaccgcca gatcggattt atgggtcggg ctggacgcca gcgccacatc atcaataaag
    16141 gaatcaacat cacgcgctgg aagcaaacaa cctaatggcg agacgcgcat cgctgcgccg
    16201 ttggttacgc cgttgttttc cagttcagca acgggtttac cgtcgcgaat ggcgttaagc
    16261 gcaatcttcg aggtcggacc tagtacgttt ttgttaaagg cgtcgaaacg caacgcccag
    16321 tcgagaatat tacgcccaat cagattcgga tcgatcttgc cttcacattc cagtaacgca
    16381 tccgccagac acaggccatc gaggtatcgt cggtgaattc ggcgcggtta aaataacagg
    16441 ccgcgttatt ctcctttggt ccaggcagaa aacggtcaat ccagccaaag tgtgctttaa
    16501 cgcggctgcg tggccaaagc tcggagggca tccccatcgc atcccctaac gcctgcccat
    16561 aaagagcacc gagaatacgt tctgttttca tttaacttcc ccttgtgtca acgcaatatc
    16621 tcgatcatcg accttgatag ccgtaatttc gttgtcggat tcgcggaaaa aaatcatgaa
    16681 cagcacggcg ataatggcaa tcatcaccgc gccgaaagtc cacatcccgg accagttgaa
    16741 agtcagtccg tttaccggtt cctgataagc gaacatcttt tccatcatca caccgccaag
    16801 acgatagccg agcaaactgc cgaagccctg gcagcagagc gtgatcagcc cctgcgcagc
    16861 ggtacgcata tgcacggggg cttttttatc gacatagatg taagcggtaa cgtagtaaaa
    16921 atcgtaactt acgccgtgaa gcaaaatacc gaggaacagt aacgcgtagg tgaaattgac
    16981 aggtaatgta aggtacgcga tgacaatacc acataataat accttttgaa tacctttgcg
    17041 gtgaaaactt gaccgcgcta acattttttc tgatgaatcg agccaacaga aaacgctgaa
    17101 aaaacatcca aaagatggaa aaactcgccc gtaacgacca ggattatgat tgcccgcaaa
    17161 aggcgggcca ggacaatcca tagccgatat ccaatcggaa tttacgggag catagtaatg
    17221 acagatattg cgcagttgct tggcaaagac gccgacaacc ttttacagca ccgttgtatg
    17281 actattcctt ctgaccagct ttatctcccc ggacatgact acgtagaccg cgtgatgatt
    17341 gacaataatc gcccgccagc ggtgttacgt aatatgcaga cgttgtacaa caccgggcgt
    17401 ctggctggca caggatatct ttctattctg ccggttgacc agggcgttga gcactctgcc
    17461 ggagcttcat ttgctgctaa cccgctctac tttgacccga aaaacattgt tgaactggcg
    17521 atcgaagcgg gctgtaactg tgtggcatca acttacggcg tgttggtgtc ggtatcgcgg
    17581 cgctatgcgc atcgcattcc atttctcgtc aaacttaatc acaacgagac gctaagttac
    17641 ccgaatacct acgatcaaac gctgtatgcc agcgtggagc aggccttcaa catgggcgcg
    17701 gtggcggttg gtgcgactat ctattttggc tcggaagagt cacgtcgcca gattgaagaa
    17761 atttctgcgg cctttgaacg cgcgcacgag ctgggcatgg tggcagtgct gtgggcctat
    17821 ttgcgaaact ccgcctttaa gaaagatggc gttgattacc atgtttccgc cgacctgact
    17881 ggtcaggcga accatctggc ggcaaccata ggtgcagata tcgtcaaaca aaaaatggcg
    17941 gaaaataacg gcggctataa agcgattaat tacggttata ccgacgatcg cgtgtacagc
    18001 aagttaacca gcgaaaaccc gattgatctg gtgcgttatc agttagctaa ctgctatatg
    18061 ggccgggctg ggttgataaa ctccggcggt gctgcaggcg gtgaaactga cctcagcgat
    18121 gcagtgcgta ctgcggttat caacaaacgc gcaggcggaa tggggctgat tcttggacgt
    18181 aaggcgttca agaaatcgat ggctgacggc gtgaaactga ttaatgccgt gcaggacgtt
    18241 tatctcgata gcaaaattac tattgcctga tacgcctcat acgaacagca ccaccccgta
    18301 ggacggataa ggcgtttaca ctgcatccgg caacctatgc ctgatgcgac gcggaagcgt
    18361 cttatcatgc ctacatagca ctgccacgta tgtttacacc gcatccggca taaaaacacg
    18421 cgcactttgc tacggcttcc ctatcgggag gccgtttttt tgcctttcac tcctcgaaca
    18481 attttcatat tgtcgttttt gtgatcgtta tctcgacatt taaaagcaaa taatttcatt
    18541 atattttgaa atcgaaagca aacgacaaga tatgaaaatg tacgtggtat cgacaaagca
    18601 gatgctgaac aacgcacagc gcggcggtta tgcggttccg gcattcaata ttcacaatct
    18661 cgaaacgatg caagtggtgg tggaaaccgc tgccaacctg catgcgccgg tcatcatcgc
    18721 cggaacgcct ggcacattta ctcatgctgg tacagaaaat ctgttggcgc tggtcagcgc
    18781 gatggcgaag caatatcacc atccactggc aattcatctc gaccatcaca cgaaatttga
    18841 cgatatcgct cagaaggttc gttctggcgt gcgctcagtc atgattgacg cctcgcattt
    18901 gccttttgcg caaaatattt cacggctcaa agaggtggtg gatttttgcc atcgctttga
    18961 tgtcagcgtc gaggcggagc tggggcaact tggcggccag gaagatgatg tgcaagtcaa
    19021 tgaagccgat gcgttgtaca ccaacctcgc acaggcgcgt gaatttgccg aggcaaccgg
    19081 gattgattcc ctggcggtcg ccatcggcac ggctcacggt atgtatgcca gcgcaccggt
    19141 gcttgatttt tctcgactgg agaacattcg ccagtgggtg aacttaccgc tggtgctgca
    19201 tggcgcgtca ggattatcga ctaaggatat tcagcaaacc atcaaactgg ggatatgcaa
    19261 aatcaacgtt gcaacggagc tgaaaaatgc cttctcgcag tcgttaaaaa attacctgac
    19321 cgagcacccc gaagcgaccg atccccggga ttatttgcag tcggctaaat ccgcaatgcg
    19381 cgatgtggtg agcaaagtga ttgccgattg tggctgcgag ggcagggcat aacgcacctg
    19441 ccatttaaca aggaaaaaac atgaaaacgt taattgcccg gcataaagct ggtgaacata
    19501 tcggcatatg ttcagtctgt tctgcccatc cgttggttat cgaagcggcg ctggcatttg
    19561 atcgcaacag cacgcgcaaa gtgctgattg aagcaacgtc aaaccaggtc aatcaatttg
    19621 gcggttatac cggaatgaca ccggcagact ttcgcgaatt tgtttttacg attgccgata
    19681 aagttgggtt tgcacgcgaa cgcattattc tcggcggcga tcatctgggg ccaaactgct
    19741 ggcagcaaga aaatgcggat gcggcgatgg aaaaatccgt cgagctggta aaagcatatg
    19801 ttcgtgctgg cttcagtaaa attcatcttg atgcgtcaat gtcctgcgcg ggggatccca
    19861 taccgttagc accagaaacg gttgcggaac gagctgttgt gctttgcttt gctgcggaaa
    19921 gtgtggcgac agattgccag cgtgagcaac tgagctatgt cattggcact gaagttccgg
    19981 ttccgggcgg tgaggccagc gccattcagt cagtacacat cacccatgtt gaagatgccg
    20041 ccaatacttt acgtacgcat caaaaggcct ttattgcccg tgggctgaca gaggcgttaa
    20101 cacgtgtgat tgccatcgtg gtgcagccgg gtgtggaatt tgatcacagc aatattatcc
    20161 attatcagcc gcaggaagcg caggcgctgg cgcaatggat agaaaacacc cgaatggttt
    20221 atgaagcaca ttctaccgat taccagaccc ggacggctta ttgggaatta gtccgcgatc
    20281 actttgcaat attgaaagtc ggtcccgcat taacctttgc tttacgcgag gcgatatttg
    20341 cactggcaca aattgagcag gaacttatcg cccccgaaaa tcgcagcggt tgcctggcgg
    20401 taattgaaga agtgatgctc gacgaaccgc aatactggaa aaaatattat cgcacgggtt
    20461 ttaacgattc attactggat attcgttaca gcctgtcgga tcgtattcgt tattactggc
    20521 cgcatagtcg gattaaaaat agcgttgaaa cgatgatggt gaatctgcaa ggcgtggaca
    20581 tcccactggg catgattagt cagtatcttc ccaaacaatt tgaacgcatt cagtccgggg
    20641 aattatcagc aatgccgcat cagcttatta tgaataaaat ttatgatgtt ttgcgcgcct
    20701 atcgctacgg ctgtgcggaa taaggacggt atatgactaa cctgtttgtt cgtagcggaa
    20761 tttcttttgt cgatcgtagc gaagttttaa cctatatcgg taatgagatg ctcgcgaaag
    20821 gtgtggttca tgatacctgg ccacaggcat taattgccag agaagcagaa ttccctaccg
    20881 ggataatgct tgagcagcac gccattgcaa taccgcattg tgaggcgatt catgctaagt
    20941 cgtcagccat ttatctgtta aggccaacaa ataaagttca ttttcagcaa gcggatgatg
    21001 ataacgacgt ggcggtatcg ttggttattg cgttgattgt ggaaaatccg cagcagcaat
    21061 tgaaactttt acgctgttta tttggcaagt tacaacagcc cgatatcgtc gagacactaa
    21121 ttactcttcc tgaaactcag ttaaaggaat acttcacaaa gtatgtttta gattcagacg
    21181 aataaatccc tctgtaacaa taaaaaggac tatttatgaa acgcaagatt attgtcgctt
    21241 gcggaggcgc ggttgcgacc tctacgatgg cggcggaaga aattaaagag ttgtgtcaga
    21301 gtcataatat tcctgttgaa ttaatccagt gtcgggttaa tgaaatagaa acctatatgg
    21361 atggtgtgca tttgatatgt accactgcca gggtggatcg tagttttggc gatattccgt
    21421 tagttcacgg catgcctttt gtttctggtg tcggtatcga agcattacaa aataaaattc
    21481 tgactatctt acaggggtga cctatgtttt cagaagtcat gcgttatatt ctcgacctcg
    21541 gccctacagt gatgctgccg gttgtcatca ttattttttc taaaatatta ggcatgaagg
    21601 caggcgattg ctttaaagcg ggtctgcata tcgggattgg ctttgttggc attggccttg
    21661 tgattggctt aatgctggat tccattggtc cggcggcgaa agcgatggcg gaaaatttcg
    21721 acctgaatct gcatgtggtc gatgtcggct ggccgggctc ttcaccaatg acctgggcgt
    21781 cgcaaattgc gctggtggcg attccgattg cgatattggt taacgtggcg atgttactga
    21841 cccgtatgac gcgggtggta aatgttgata tctggaatat ctggcatatg accttcaccg
    21901 gcagcgttgc tgcacctggc aaccggttca tggatgatag ggatggcggg tgtggtaatt
    21961 cacgcggcgt ttgtttataa gctcggcgac tggtttgccc gcgatacccg aaatttcttt
    22021 gagctggaag gtattgctat tccgcacggt acgtcggcgt atatggggcc gattgcggtg
    22081 ctggtcgatg ctatcatcga gaaaatccca ggcgttaacc gaattaaatt tagcgccgac
    22141 gatattcagc gcaaatttgg cccttttggc gaacctgtca ccgtgggttt tgtgatgggg
    22201 ctgattatcg gcatcctcgc gggttacgat gtcaaaggtg tattgcagct ggcggtaaaa
    22261 acggcggcag ttatgctgct gatgccacgg atgattaaac ccatcatgga tggtttaacg
    22321 cccatcgcta agcaggcccg tagtcgttta caggcgaagt tcggcggtca ggagttcctg
    22381 attggcctgg atcccgcatt actgctgggg catacggcgg tggtatcggc aagcctgatt
    22441 tttattccgc tcaccatttt aattgctgtt tgtgttccgg gtaatcaggt actgccgttt
    22501 ggcgatcttg ccactatcgg cttcttcgtg gcgatggcgg tcgcggtgca tcgtggaaat
    22561 ctgttccgca ccttaatctc gggtgtcatc attatgagca tcaccctgtg gatcgcgacg
    22621 caaactattg gtttgcacac ccaactggcg gctaatgctg gggcgttaaa agccgggggt
    22681 atggtggctt caatggatca gggcggttct cccattacct ggttactgat tcaggttttc
    22741 tccccgcaaa atattcccgg tttcattatt atcggcgcaa tttatctgac cggtattttc
    22801 atgacctggc gtagagcgcg tggttttact aaacaagaga aagccgttct cgcagaataa
    22861 tttttacctg agggggaggt tatccccctt aacaatcagg agtttttatg aaatcagtag
    22921 tgaatgatac tgatggtatc gtgcgcgttg caaaaagcgt cattcctgaa attaaacatc
    22981 aggatgaggt gcgggtaaaa attgccagct cgggcttatg tggttccgat ttacccagga
    23041 tatttaaaaa tggtgcacat tattatccaa taacgttagg ccatgaattt agcggctata
    23101 ttgatgcggt gggatccggt gttgatgatt tacaccctgg cgatgcggtt gcctgtgtgc
    23161 cgttattacc ctgttttact tgtccagagt gtttgaaagg gttttattcc cagtgcgcaa
    23221 aatatgattt tattggctcg cggcgtgatg gtggatttgc tgaatatatt gtcgttaagc
    23281 gaaaaaatgt ctttgctcta cccacggata tgcctattga ggatggggct tttattgagc
    23341 cgattaccgt tggcctgcat gcttttcatt tagcgcaagg ttgtgagaat aaaaacgtta
    23401 ttattattgg tgccggaacc attggcctgc tggctattca gtgcgctgtc gcgctgggag
    23461 caaagagtgt gacggcgatc gacattagct cagaaaaact ggcactggca aaatctttcg
    23521 gtgcgatgca aacatttaac agtagcgaaa tgagcgcgcc gcaaatgcag agcgttttac
    23581 gcgaactgcg ctttaatcag cttatcctcg agacggctgg cgtaccgcaa actgtcgaac
    23641 tggcggtaga gattgccggt cctcatgccc aactggcgct ggtgggcacg ttgcatcagg
    23701 atctgcattt aacatcggca acgtttggca aaatattgcg taaagagctg acggttatcg
    23761 gcagttggat gaactattcc agcccttggc cggggcagga gtgggaaacg gcgagccggt
    23821 tgctgacaga acgtaagtta agcctggatc cattaatcgc tcaccgtgga agctttgaaa
    23881 gcttcaccca ggcggtgcgt gacatcgctc gtaatgccat gccgggcaaa gtgttgctca
    23941 ttccatgaaa ccgcgggcca gcgtgatgct ggcccggtat tgtgcaaaac agatcattca
    24001 ccaatggtcc cccttcgttt acactagcca caattgaata tgggtaaatg acgatgaatt
    24061 cattcgagcg aaggaataag atcatccaat tagtgaatga acagggaacc gtgcttgttc
    24121 aggatctggc gggagtattt gctgcctcgg aagcgacaat ccgtgccgat ttgcgctttc
    24181 tcgaacaaaa aggcgtggtt acgcgctttc atggcggtgc ggcgaaaata atgtccggta
    24241 atagtgaaac cgagacccag gaagtcgggt ttaaagagcg atttcagctc gccagcgcgc
    24301 caaaaaacag aatagcgcag gcggcagtca aaatgatcca tgaagggatg accgttattc
    24361 tcgacagcgg aagtaccaca atgcttatcg ccgaaggatt aatgaccgct aaaaatatca
    24421 cggtgattac caacagtctc ccggcagcgt ttgccctttc cgaaaataaa gacattactc
    24481 tggtcgtctg tggtggcacg gtccgccata aaacgcgctc gatgcatggt tccattgccg
    24541 agcgttcatt gcaggatatt aatgccgatt taatgtttgt cggtgctgat ggtattgatg
    24601 cggttaatgg tattacgacc tttaatgaag gttattcgat tagcggggcg atggtcaccg
    24661 ccgcaaataa agtgattgcc gttctcgatt catcgaaatt taatcgtcgc ggttttaatc
    24721 aggtactgcc aatcgaaaaa attgacatta ttgttaccga tgatgctgtg tcagaggtgg
    24781 ataaattggc attgcagaag acacgggtaa aattaattac ggtgtagtat tgttctgtat
    24841 gccggataag gtgtttacac cgcatccggc attacggatt ctccctggtt caagagagaa
    24901 tagtttgatt aacgcaataa tggacaatct ggtggtaatc gacaacgcaa cgccgccgga
    24961 agtatttcaa tatggaagtt ttgcccactc aacggttcgc catcaagatt aaaagtgatt
    25021 tcgtgtgggg cttgtatatc aaaccacgac gaagcgcctt cgataatatt cgggttatct
    25081 tcgtcagatt ttaaggttga tacgagagcc ggaagaattt catcgccggt aaaaatgcgc
    25141 agttgcagca agccatcgtt aattaacgcg ttcgggcaca attgttgacc gccaccggcc
    25201 tgacgcccgt taccaatacc aatgaccagg gcgtcacctt gccagtgaaa gttttcaccg
    25261 cggatttcac aacggtccgg ttgcagagta tccatgcgca ttaagccatg aatgatgtaa
    25321 gagacgccac ccagcgcggc ttttaatttt tccggcgttt ctgtggtaat acgcgtccca
    25381 aatccgcctg tcgccatatt aataaaacag gtttgtttgt tgacctgcgc catatctatc
    25441 gcaatggcat taccggcaat tgccagtttc agcgccttat ccagtgcctc aggaatccct
    25501 acactggtgg caaaatcatt ggcggttcct aatggcaaaa ttcccagcgc gggtatgtca
    25561 tccccctcac actgaatcaa cgccgtagaa acttcattaa tggtgccatc gccaccaccg
    25621 gcaatcaccg ttgcgacgcc caacttccgg gcctcctcta caaatcgtgc ggcatcacct
    25681 ttctcccagg tgacccgcac atggatcgtc attccttcct cacgcaacag cataattgct
    25741 tcgcgcaagg gtagattgtc agtacttttg ccattaagaa tcagtaagct ggcgggaaat
    25801 tctgccatga tcgtatgtgc ctttatgatt ggtctgtaga tagtgtagag cagaaaacaa
    25861 aaggtggagt cagaacagga tgaaagtcgg aggataaaaa gtcagttcaa gtatggagga
    25921 ttcagtggtt accaccagga gcttgcaatg gggacagcga atactaacgg ctgccaccga
    25981 taaatttcaa aaaagagcat atacctaata ttcaactaaa cagtggcatc ttcaatataa
    26041 tatattaaag cccccatgga gttaccctga agggcctcaa tgtccgtaat tcctacttat
    26101 gtaggaaatg ttgtacagaa catttattat aatcctattc aattataata atcatgccat
    26161 tattatattt aaacactaga gagtgtcgtt ggtatttaat gggggaaggt gagatgaaaa
    26221 agatagctgc tatatcatta attagtattt ttcttatatc tggttgtgct gtgcataatg
    26281 atgaaacaag tatcggtaaa tttggtcttg catataaaag taatattcag cgtaaactcg
    26341 ataaccaata ctacaccgaa gccgaagctt ctttagccag gggcagaata tctggtgcag
    26401 aaaatatagt aaaaaatgat gcagcccatt tctgtgttac tcagggcaaa aaaatgcaga
    26461 tagttgacct gaagacagaa ggtgcaggat tacatggcgt cgctcgtctg acattcaaat
    26521 gtggagagtg agaatatttt ttggtaagcg tcaaacatgc gcgttctggc tgtgcgtagc
    26581 cggaacctgg gcgagcacga taccgttacg tgaaaggcat tgtgctatga agggggattc
    26641 tatcgatgtg gtcaatggaa gacggttatc agggatcaat ccttctttac agcttatgta
    26701 caaaaaataa gcccgtgtaa gggagattac acaggctaag gaggtggttc ctggtacagc
    26761 tagcatttta tggattatgt ttttcagcga aacggatgat aaccttaata aatgcagctg
    26821 tatgtgatcg gtttctaaga attttccatc cgggaaaaat aatcgaaatt aatcacttac
    26881 cgtgggggtt acgcgtggtt tccccggaga aattacgcat tagcagcgcg taatttagct
    26941 caagatcctg cgggaccggg agccacacag tataaccatc gcctggtgcg accggcataa
    27001 cttcgccttt ggcgttttcc atgtgctcaa gggtaaagtt aatgttgcct tgcggcgtca
    27061 tcagctcaag actgtcgcca acggagaatt tatttttcac cgctaccgct gcgaggtccc
    27121 ccttgcgctc accggtaaac tcaccaacaa actgctggcg gtcagaaact gaataaccgt
    27181 attcgtagtt ctgataatcg tcgtgagtat gacgacgcag gaaaccttcg gtatagccac
    27241 gatgcgccag accttccaga gtttccagca ggctggtatc gaacggtttt cccgcagcgg
    27301 cgtcatcgat agctttgcgg taaacctgtg cggtgcgtgc gcaatagtag aaagatttgg
    27361 tacgaccttc gattttcagc gaatgcacgc ccattttggt caggcgttct acatgggcga
    27421 tggcgcgcag atctttcgag ttcatgatgt aagtgccgtg ctcatcttca aacgcggtca
    27481 tatactcgcc cggacgctgg gcttcttcga tcataaacac tttgtcggtt ggcgcgccga
    27541 tacccagcgt cggctcaaca ttttgcaccg gaatcggctc gtacttgtgt acgatgttgc
    27601 caacgtcatc ttctttccct tcctggacgt tgtactccca gcggcaggcg ttggtgcagg
    27661 tgccctggtt cgggtcgcgc ttgttgatat agccagagag caggcagcga ccggagtagg
    27721 ccatgcacag tgcgccgtga acgaagatct cgatctccat atccggcacc tgattatgga
    27781 tctcttcaat ctcttccagc gacagctcgc gagagaggat cacgcgggtc agtcccattt
    27841 gctgccagaa tttcaccgtc gcccagttca cggcgttagc ctgcaccgag aggtggatcg
    27901 gcatttctgg gaagtgctca cgcaccagca taatcagccc tggatcggac ataatcagcg
    27961 catccggccc catttccacc accggtttca ggtcacggat aaaggttttc agcttggcgt
    28021 tgtgtggtgc aatgttgacc acgacataaa actttttccc cagcgcgtgg gcttcattga
    28081 tgccgagctg aagattttcg tggttgaatt cgttgttgcg cacacgtagg gagtaacgcg
    28141 gctggcccgc ataaacagca tctgcgccat aagcgaaagc gtaacgcata tttttcagcg
    28201 ttcccgccgg ggaaaggagt tccggtttaa acataattct ctcgttctga tgacaggtca
    28261 gatccgcttc acctgatgaa gcggttaggg gaggccccca ctttaagggc gggcattgta
    28321 gcgctgtagg ggggatctgg taaagcgccg gacgccaaca acccacgccc ggcatcgtaa
    28381 ttacgtattg tctttcaccg tctggcttgt gccattggct ttaacggagg caattccggt
    28441 ttcacgagat tgcgcggtgg cgtacatctg gctggagccg ataatttgat gattagcggc
    28501 tttcagattg aaatagaatt tgccgttttt ttctcatagc gctcttcttg cgggctgttg
    28561 ctacgcaccg acgcgatgcc cttttccgca gaggcttttg aggtatacag ctcgctggtg
    28621 aggatagtct caccattgcc cgcttttagc acaaaccgga actgattatc actgctctta
    28681 ctgagttcaa accaaccagc cataatgact ccttgattga aattgcttat cgaaaacagc
    28741 atctaaaggt gctgtttact gattaaagta tgagcgattt tctgatgcct gccagggcgg
    28801 agagataatg tcagtttgct gaccatgcgt ggaataatgt tgccggatcg ggtctgaacg
    28861 ccatctccgg ctaacaaagt aatgtcgcta aaactaaacg atgcggcagg cgtcggcttc
    28921 ccagcggtaa ccaacgccgt aaacggcgcg gataaatgac tgttcggcgt cgagagattc
    28981 cagcttgcgg cgcagatttt taatgtggct gtcaatggtg cggtcggtta ctacgcggta
    29041 gtcgtcataa agatgattga gcaattgctc gcgggagaac acttttcctg attcgtgaga
    29101 gagcgtttta agcagacgaa attccgcagg cgtcaggtca agcattttac cgcgccatga
    29161 agcctgaaaa cgaccttcgt cgataatcaa cgggctttcg gcatcctgtt gctgaagttc
    29221 gcgttgcggt ttacaacggc gcaaaatggt tttgacacgc gctaccactt cccgtgggct
    29281 gtacggcttg cagatgtagt catcggcgcc aatctccagc cccagcaggc ggtcgatctc
    29341 ttcgattttt gccgtcacca tcacgatcgg aacatcggaa aaacgacgaa tttcccggca
    29401 taatgtcagg ccatcggtgc cagggagcat cagatccaac aggatcagat ccggtggtgt
    29461 ctggcgcaca tacgccagta cctgatcgcc gtggctgata agcgtcggcg catagctcgc
    29521 agcacgcaga taatcaatga gcaactgccc cagcttcggt tcatcttcca cgatcaaaat
    29581 acgcggtgtg ttttcgtcga ttggtaactc ggtcatactt ctctctgtaa atcccgttcc
    29641 agcggtaact ctactgtaat gcttaccccg ccaaaaggcg aatgggcggc aataatgcgg
    29701 ccattatggg cttcaacaat gttcaggcaa atcgccagcc ccagcccgga accgccgctg
    29761 gcgcggttgc gggaaccttc ggtgcgataa aaacgttcaa acaatttttg tagctgatcg
    29821 tcactgacac ctggcgcact gtcggcaaag gtcaggcgca ccgttttgtc gtgctgctcg
    29881 gcagagattt tcaggctgcc gccgctgtca gtgtagcgca ggctgttttc cagtaagtta
    29941 ttgaataact gcattaaacg gtcgcgatcg ccaaatacgg tgatactgtc tggcagggaa
    30001 aattgcagtt tcaggccacg gctggcgaat cgttcgcgaa atgcgccgcc cgccacttcc
    30061 agcagtggga tcaaatctac cggtgctttt tgataggcga gagcgccttc atcagacatc
    30121 gacaactgat gaagatcgtc aaccagtttg gtcagtgtac cgacctccgc ctgtaaagac
    30181 gccaccgtct ccggcgtgaa tttgcgcacg ccatcctgaa tggcttccag ttcaccgcgc
    30241 agcaccgcta atggcgtacg cagttcgtga gaaatatcgg ccataaaatc gcgccgcatt
    30301 tgctggtttt tctccagcgt gctggcgagc tggttgaagt cttgcgccag tttgcccagt
    30361 tcatcttcac tggtgggcgt tacgcgggta gtgaaatcgc ccgccgccag tttgtgcgtg
    30421 ccatcgataa gtcgttttac cggtgccagc aaaccgcgcg ccagcagaaa ggtggcgagc
    30481 gccgcgagta acgttgccag ggcgacgatc agccagctgg tttgccgctg ttgtttatcg
    30541 aaattgatat cagtattgcg ggtcagccgc tcaacagggg aggcaatcac cgcgcccact
    30601 tccgcaccgt tgaccagaat gggtcgtcgt gtaccgtcag gtggaatcgg cgctcgcgga
    30661 ccaaccagta ctttgttgtt ttgatcgacc acccagaact gggtacgcca gccgtgcggc
    30721 ggcatacccg ggccgggttt atcttccgaa ttatcgtgtt caaatgaacg caggatctga
    30781 aagacaaagc gatcgttgtt gcgcaggaag cgccagttac catgctgcgc atactgctcg
    30841 ccaagcgcat cacttaacag ctgtaatcgc tgttcattgc cgtgtttgat gtaatcaata
    30901 aagccacgct caaaactgat acgcaccgcc cagtgcatac tgatcagcaa gacaatgcag
    30961 gtggcgaaaa tcgccagaaa cagtttgccg gtaataccgg gtcgccagaa cttcattgcg
    31021 cgctcctttt tcgccgcgaa atagctacat tttgatgcgt atcgttcggc actctggcaa
    31081 agatgaacgc cggaagggcg atgataaacg ccatgctaag ccaggtgtac ataaagacgg
    31141 tttgtgtggt gccgctgtcg acgctgacat gctgcgaacc aaaaagcccc agcaacagcc
    31201 cggcgatggt gacgccgata ctcatcgaca attgcataat catcgacagc aggctgtttc
    31261 cgctgctcgc cagattgtcc gggagatctt tcagcgtcag ggtgttcatg gaggagaaac
    31321 gggtcgagtt gaccatccct tgtaaaaaca ggacgaacgg caaaacgtag taccagccca
    31381 acagagcggt agtcataaac aacagggtga ccagcgacag acccagcgtg gtcgctacca
    31441 gtacccgacg ataaccaaag cgattcacca cctgtaccac aattcgcttc attcccatgc
    31501 tgccaagcac catcgggatc atcatcagtc cggcatgaaa cggcgagaaa ccgaggccaa
    31561 tttgcaggaa aaccggtgtc ataaagggca acatgccact gccaatacgt ccggcaaagc
    31621 tccccgccag gcccagcgaa aaggtacgag tgcggaacag tttcagactg aacagggcgc
    31681 ggttgttatt tctggcgtgc agcagataaa gcaccagtgc caccacgcca actgcgacca
    31741 ggccagcaat cgccagcggc gataaacctg tacctttact gccgtccagc gccagggtta
    31801 ataccgccat gccaaccgcc agcaataaaa agccggaaag atcaaagcgc cgcgtctgca
    31861 tggtgtagtt cggcattaac atcaatgtgg cgatcgcacc gataatcccc accggaatgt
    31921 tgatcaaaaa gatccagtgc cacgacgcgt attctaccag cagaccgccg agcgccggac
    31981 cgagcagcgg accgacctga ccgggtaacg tgacaaaggt catcgccgcc atatattgct
    32041 cgcgcggtac gattttcatc accgtcaacc tgccgaccgg caccatcatc gcaccgccaa
    32101 cgccctgtaa cgcgcgtgcc agcaacagtt cgttcagcgt gccggaaagc gcgcaaaaca
    32161 gtgaaccgag agtaaacagc acgatggcgg taaagaaaat attgcgcacg ccgactttgt
    32221 ccgccagcca gccgctggcg ggcagcatca ccgccacggt cagcacataa gagacaatga
    32281 ccatgtgcat atgcaacgga ctttccccga ggctttgcgc cattgaggga agggcggtgt
    32341 ttacgatggt ggtgtccagc gactgcataa agaagccgaa agccacaatc cataattgcc
    32401 agcgggtgct gtcgggaaga tctgtcattt actcggttac cgtttgttta ggtttacgcg
    32461 aaaaacgcag ccgcagacgg tcgaaaaaga gatacaccac cggcgtggta tacagcgtca
    32521 ggagctggct catcaccaga ccgccgacaa tggtgatcct caggggttgc cgcagctccg
    32581 agccgtcacc gcccgacaat accagcggca gcgcgccaaa cagcgccgcc agggtggtca
    32641 tcataatcgg gcgaaaacgc agcagacagg cctggaaaat agcttcctgc ggcgtcaggt
    32701 taccgtgccg ttgggcttca agcgcaaaat cgaccatcat aatggcgttt ttcttcacga
    32761 tgccgattaa tagcatgatc cctatcaggg cgattaggct gaacggggca ttgaacagct
    32821 ccagcgccaa cagcgctcca acgcccgccg agggcagggt ggagagaatc gtcagcggat
    32881 gtacgtaact ctcgtaaagg atacccagca cgatatacac cgtggcgatg gcggcgataa
    32941 tcaggatcac ctgcgagttc atcgtctcct ggaaaacctg cgccgtgccg gcaaaactgc
    33001 cgcgcaccgt cgaaggcaca ccaagctggg tcattgcgcg atcgatcgcc gcactggcgt
    33061 ccgagagcga ttttccggtc ggcaggttga acgagatggt cgaggccgcc gataatccct
    33121 gatgattcac cgatagtggg gcattcgccg gttgccattt agcgaaatac gatagcggga
    33181 tcgctttgcc ttcgttattg ataacgaaca ttttttccag cgcactgatg tcctgggtat
    33241 agcgcggatc cacttccatc accactttat actggttcat cggctggtaa atggtcgaga
    33301 tttgccgctg accgaaggcg ttatttaaca gactgttggc ggcctgtacg tcgattccca
    33361 gccgtgccat ggtgtcgcgg tcgtaaacca gattcatctc cgcgccgtta tcctgctgat
    33421 cggagttcac gtccgccagt tccggcaacg tcgccagttt tttgcggatt ttcggctccc
    33481 attcgcgcag tgccgccagg tcgtcggata acaacgtgta ctggtagctg gcgttcgact
    33541 gacgcccacc aacgcgaata tcttgtaccg ccatcaggaa cagattcgcc cccggttctt
    33601 tcgccagctt cacgcgcaga cggtcgataa tttgctgcgc cgtttcgctg cgttcgtcgc
    33661 gtggcttgag ggtgataaac atcatcccac tgttaactcg tgaaccgccg gtaaagccgg
    33721 tgacattatc cactgccgga tcgtcacgga taattttcat gaaatcctgc aatttgccgc
    33781 gcatcgcctg aaacgaaata ctctgatccg cctgtatccc acccatcagt acgccggtgt
    33841 cctgctccgg gaagaaggtt ttcgggatcg agatattcag cgcaatggtg ccaagcagca
    33901 ccacgcccac cagacgggta tgattgagca cccatttcag cgacttgccg tagccttgtt
    33961 gcagggctac caacatgcgg ccaaaaccac gcagtcgctt ttgctcacgc ggcttgctgg
    34021 cttttagcat ccagccgcac atcattggcg ttagcgtcag ggaaaccagc aacgaaatac
    34081 caatggcgac agaaagcgtc acggcaaact cgcgtaacag tcggcccggc aatccgccca
    34141 tcaacagcag cggcaggaac accgccacca gtgacagact catcgacagc accgtaaagc
    34201 cgacttcgcg agtaccttgc agcgcggcct gtagtggttt catccccgct tccagatgac
    34261 gggcaatgtt ttccagcacc acaatggcgt catccaccac gaaaccagta gcaatggtga
    34321 gcgccatcag cgaaagatta ttgagactga atccgcacag gtacatcgcc gcaaacgtac
    34381 caatcagcga aaccggcacc gcaacggcgg gaataatagt ggcgcgaccc gagcgcagga
    34441 ataaaaagac caccagaatc accagcgcca ccgagataat cagcgtttgc tcgacttctt
    34501 ccagcgaggc gcgaatggtg ggggagcgat cctgggcaat ttgcagatca atcgccgccg
    34561 gaatggtctc ctgcaactct ggtaattttg cccggatgct gtctaccgtc tggataatat
    34621 tggcttccgg cagtttgcgg atcatcagta aaatagccgg tttggcgttg gtcatcccgg
    34681 cgttgcgcac atcctgcact gagtcagtca ccgtcgccac atcgcccaga cgaaccgcgc
    34741 cgccgttgtt gtagtgaata atcagcggct gatattcagc ggcggttttc agttcatcgt
    34801 tggtctggat ctgccagcgg tgagtgccat cttccagcgc cccctgcggt ttacgcacgt
    34861 tggcattgct gatggcagtg cgtacgtcgt ccagcgacac gccctgatta aacagcgcct
    34921 gcgggttcag cccgacgcgc acggcgggca gcgagctgcc accgacatcg acatcaccga
    34981 caccgtcgat ttgcgaaatc gtcggagcca gctgcgtcga ggcgaaatcg tacagttcac
    35041 cctgcgaata agtatcggat gtcagcgtga ggatcataat tggcgcatcc gacgggttcg
    35101 ctttgcgata ggtcgggcgg ctgggcatcc cgctgggcag caaactttgt gcagcgttga
    35161 tcgccgcctg cacatcacgc gctgcgccgt tgatatcccg gtcaaaatca aactgcaaaa
    35221 taatgcgcgt gctgccgagc gaactgctgg aggtcatttc actaaccccg gcaatgcgcc
    35281 caagtgagcg ctccagcggc gtggcaacgg aagacgccat tgtctccggc gacgcaccgg
    35341 gcagcgaggc gctgaccata atcaccggaa aatcgacctg cggcagcggg gcgaccggca
    35401 gcatacggaa acccagtatg ccgcacaggg taatggcgac cgacagtaaa atcgtcgcca
    35461 ccgggcggta aatgaagagg gcaaaaaact tcacttacgc ctcctcttca tggcgagcaa
    35521 agcgactttt ggtccacaat accaagcggt cgaacagcag ataaatcacc ggcgtggtga
    35581 acagcgtcag cacctggctg acaatcagac cgccgaccat gccgatacct aacggacgac
    35641 gcagttccgc gccgaccccg gtactcaaca tcagcggcag cgcgccaagc agagccgcca
    35701 gggtggtcat caggatcgga cgaaaacgca acagacaagc ctggtagatt gcatcgcgcg
    35761 gcgacatgcc ttgctcgcgc tcagcagcca gcgcgaagtc gatcatcatg atggcgttct
    35821 tcttcacaat accgatcagc aaaataatgc cgataatcgc aatcacatcc agttcgctac
    35881 cggcaatcat taatgccagc agcgcgccga cccctgcggt gggtagcgtc gagagaatgg
    35941 tgatcgggtg aataaagctc tcgtacagaa tgccgagcac gatatacatc gccaccaccg
    36001 ccgcgacaat cagccagaca gtgctgccca gcgccgactg gaaggcgagg gtgctgccct
    36061 ggaattgcgt ggtgatatcc accggcagat tcagcgtctt ttcggtgtcc ataatcgcct
    36121 gcaccgcatc gcccagcgaa tagttatccg gcacgttaaa ggagatggtt gttaccggga
    36181 actgatccag atggttgatg gaaagcggcg caaaacgctg ctcaattttg gcaattgagc
    36241 ttaacggcac cacgccaccg tcgctgctgg tcaggcgaat ggtatccagc gccgcgaggc
    36301 ctggggtatt ttcggtattg tgctccagca ccacgcgata ctggttagcc tgagtataaa
    36361 tagtggaaat cagtcgctga ccaaacgcgt tgtacagggc gttatcgacg tccgccatgc
    36421 tgatccccag acggctggcg ctgtcgcgat caacattgac atacgccacc agccctttgt
    36481 cctgccagtc gctggagaca tcagaaagct gtggcagttg ctggagtttt tccatcaact
    36541 gtggcaccca ggtactgagc gcatccagtg acgtggcctg caaggtaaac tggtactggg
    36601 tgcggctgac ctgagtatcg atggtcaggt cctgcgttgg ttgcaggaag agatcgacgc
    36661 ccggcacttt atctaccgcc gtttgcagac gggcgatgac tttttgcacc cgatcatcac
    36721 gttcatccaa cgatttgagg ttgatttgta aacgtgcact attcagcgac gggttagtgc
    36781 catcaacgcc aacaaatgag gtcaggcttt gcactgccgg atcctgcaaa atcacgtccg
    36841 cgacctggcg ttgtcgctgg gccatattgg caaaggagct ggattgcggt gcctgcaaag
    36901 tgccctgaat aatgccgttg tcctgtaccg ggaagaaacc tttcggaatg aacacccaca
    36961 gcagcacgct aagcagcagc gtgctgagtg ccacgcttaa ggtcagccac ggatgattca
    37021 gcactttcgc cagtccacga ccataggcgg cgattatcct gtcgaacatt ttttccgagg
    37081 cacgggagaa gcggttctgt ttacgcaacg actcctggct gagcatccgc gcgcacatca
    37141 tcggtgtcag ggtcagcgac accaccgctg agatcaaaat cgctaccgcc agggtaatag
    37201 caaattcgcg gaacagtcgc ccgacgatat cgcccataaa cagcagtggg atcaacaccg
    37261 caatcagtga gaaggtcagc gagataatgg taaagccgat ttcacctgcg cccttgagcg
    37321 ccgccgccaa cggtttttcg cctttttcga tatagcggga aatgttttcg atcaccacga
    37381 tggcgtcatc gaccacgaat ccggtggcga tagttaacgc catcagtgtc aggttattga
    37441 ttgaaaaatc gagaaacacc ataaccgcga aagtgccgat taacgacagc ggtacagcaa
    37501 caccgggaat gatggtcgcc ggaatattgc gcaaaaacag gtagataatc atgactacca
    37561 gcgcgatagc catcatcaat tcaaactgag tatcatcgac ggatgcgcgg atattggtgg
    37621 tgcgatcgga aagcactgtc accttcaccg atttcggcag actctcagtg agctgtggca
    37681 gcatctgccg aatgctgtcg gcggtggaga taatgttagc accgggctgg cgctgaacat
    37741 tcatcacaat ggcctgttct ttgttcgccc acgcgccgag ccagctgttt tctgcacctt
    37801 gctctacagt tgctacatcg cccagacgaa ttggcgcgcc gttctggtag gcgatgatta
    37861 gctggcgata ctcttcggcg gattgcatct ggtcgttcgc ggaaagcgtg accgcacggg
    37921 aagggccgtc gaggctacct tttgccgagt taacgttagc gccggtaatg gcggtgcgca
    37981 cggtttcgct ggtcaggccg agggcggcaa tcgcctgagc gttaagtttg acgcgaacag
    38041 ccggacgctg accgccggaa agcgtcacca ggccgacgcc ggaaatctgc gagattttct
    38101 gcgcgacgcg ggtttccacc atatcttcca cttgcgtcat cggcatggcg gttgaggtga
    38161 cggcgagcgt catgatcggc ggatctgccg ggttcacttt gctgtaaacc ggcgggttag
    38221 gcagatcgct cggcaacaag ttggtcgcag cgttaatcgc ggcctgcact tcctgctcgg
    38281 cgacattgag cggtaatgtt agctggaact gcaaagtgat aactgacgca ccgccggaac
    38341 tttgcgacga catctgtttc aggccagaca tttgcccgaa ctggcgttcg agcggcgcgg
    38401 taacggcaga ggtcatgaca tccgggctgg cacttgggta gagcgtgacc acctgaatgg
    38461 tcggatagtc cacttccggc agcgccgaaa cgggcagggc gcgataaccg ataatcccgg
    38521 cgagtaagat cgccaccatc agcagcgtgg tggcgacagg acgcataata aacaggcgcg
    38581 acgggccgcc tgtgctgctc gggggtaaca cctgcatcag gagcgtgctc cttttttcgc
    38641 gtattcgcgg ctggtggctt tctcttccgg agtggtggcg ctctgggctt ccaccacttc
    38701 cactttctga ctgtcctgaa tgcccggcgt caccagatgt ttgctgacct tgttttcgct
    38761 attcagcacc cagacaaaat ggccttcatt gcccatttgc agggcggctg ttgggatcac
    38821 tacggcgttt tgttcggtgt cgactaacat gcgcgcgtta acaaactgat tgggaaacag
    38881 cgcatcatcc tgattattaa agcgtgcttt cactttaatc gtaccggttg tggcatcgat
    38941 ttggttatcg aggcttaaca gcgtgccttc acttaatttc ttcgaattgg tgcgatccca
    39001 ggcttctacc atcagcggtt ttccggcttt ttgcgcctgc actacagtag cgatatcgct
    39061 ttccggcagg gtaaagacta aatcgatagg atgcgtctgg gtgatcacca caattccggt
    39121 ggtatcacca ctggagattt ggttaccaac atcaacttgc ttgagaccaa cgcgaccatc
    39181 gactggtgcg gtgatgcggc tccagtcgag ttgcaactgc gcgctggcga cgcttgcttc
    39241 atcagcctta atggtgcctt cggtttcact gaccagcgcc tgttgggcat ccagctcctg
    39301 acgggagacg agattggttt ttgccagttg ctgataacgc gccagatcac ggcgggcgtt
    39361 agcaagcgtg gctttatctt ttgccagttg gccctgggtt tgtgctaatg caactttgaa
    39421 ctggctgggg tcaatttctg ccagtaaatc gcctgctttg acctgctggc cttcctggaa
    39481 atgtaacgcc atcagttgac cgtccacgcg gctgcgcacc gtaacagtat tagcggcggt
    39541 aatggtgcca agcccggt
//
LOCUS       DAJTYG010000026        38773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTYG010000026 DAJTYG010000000
VERSION     DAJTYG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.026"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..302
                     /locus_tag="OO568_001482"
     CDS             <1..302
                     /locus_tag="OO568_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6640885.1"
                     /translation="LAYLQQAATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAA
                     AQGNFAAHFYLGRMYQNGDGVERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            355..2085
                     /locus_tag="OO568_001483"
     CDS             355..2085
                     /locus_tag="OO568_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640886.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSDPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDEIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(2145..2636)
                     /gene="yajQ"
                     /locus_tag="OO568_001484"
     CDS             complement(2145..2636)
                     /gene="yajQ"
                     /locus_tag="OO568_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6640887.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2804..3715
                     /gene="panE"
                     /locus_tag="OO568_001485"
     CDS             2804..3715
                     /gene="panE"
                     /locus_tag="OO568_001485"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6640888.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARTHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3678..4268
                     /gene="yajL"
                     /locus_tag="OO568_001486"
     CDS             3678..4268
                     /gene="yajL"
                     /locus_tag="OO568_001486"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6640889.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(4322..5770)
                     /gene="thiI"
                     /locus_tag="OO568_001487"
     CDS             complement(4322..5770)
                     /gene="thiI"
                     /locus_tag="OO568_001487"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6640890.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5976..6218
                     /gene="xseB"
                     /locus_tag="OO568_001488"
     CDS             5976..6218
                     /gene="xseB"
                     /locus_tag="OO568_001488"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6640891.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            6218..7117
                     /gene="ispA"
                     /locus_tag="OO568_001489"
     CDS             6218..7117
                     /gene="ispA"
                     /locus_tag="OO568_001489"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6640892.1"
                     /translation="MDFPQQFEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            7142..9004
                     /gene="dxs"
                     /locus_tag="OO568_001490"
     CDS             7142..9004
                     /gene="dxs"
                     /locus_tag="OO568_001490"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6640893.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTICVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            9059..10033
                     /gene="yajO"
                     /locus_tag="OO568_001491"
     CDS             9059..10033
                     /gene="yajO"
                     /locus_tag="OO568_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6640894.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRGDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPMARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(10087..10605)
                     /gene="pgpA"
                     /locus_tag="OO568_001492"
     CDS             complement(10087..10605)
                     /gene="pgpA"
                     /locus_tag="OO568_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6640895.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10583..11560)
                     /gene="thiL"
                     /locus_tag="OO568_001493"
     CDS             complement(10583..11560)
                     /gene="thiL"
                     /locus_tag="OO568_001493"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6640896.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(11638..12057)
                     /gene="nusB"
                     /locus_tag="OO568_001494"
     CDS             complement(11638..12057)
                     /gene="nusB"
                     /locus_tag="OO568_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6640897.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(12077..12547)
                     /gene="ribE"
                     /locus_tag="OO568_001495"
     CDS             complement(12077..12547)
                     /gene="ribE"
                     /locus_tag="OO568_001495"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6640898.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12639..13739)
                     /gene="ribD"
                     /locus_tag="OO568_001496"
                     /pseudo
     CDS             complement(12639..13739)
                     /gene="ribD"
                     /locus_tag="OO568_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
     gene            complement(13743..14192)
                     /gene="nrdR"
                     /locus_tag="OO568_001497"
     CDS             complement(13743..14192)
                     /gene="nrdR"
                     /locus_tag="OO568_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6640899.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            14343..14882
                     /locus_tag="OO568_001498"
     CDS             14343..14882
                     /locus_tag="OO568_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6640900.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            15181..16065
                     /gene="tsx"
                     /locus_tag="OO568_001499"
     CDS             15181..16065
                     /gene="tsx"
                     /locus_tag="OO568_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6640901.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKIRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(16242..16589)
                     /locus_tag="OO568_001500"
     CDS             complement(16242..16589)
                     /locus_tag="OO568_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6640902.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16718..17689)
                     /gene="secF"
                     /locus_tag="OO568_001501"
     CDS             complement(16718..17689)
                     /gene="secF"
                     /locus_tag="OO568_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6640903.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17700..19547)
                     /gene="secD"
                     /locus_tag="OO568_001502"
     CDS             complement(17700..19547)
                     /gene="secD"
                     /locus_tag="OO568_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6640904.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19575..19907)
                     /gene="yajC"
                     /locus_tag="OO568_001503"
     CDS             complement(19575..19907)
                     /gene="yajC"
                     /locus_tag="OO568_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6640905.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19930..21057)
                     /gene="tgt"
                     /locus_tag="OO568_001504"
     CDS             complement(19930..21057)
                     /gene="tgt"
                     /locus_tag="OO568_001504"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6640906.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTAE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(21112..22182)
                     /gene="queA"
                     /locus_tag="OO568_001505"
     CDS             complement(21112..22182)
                     /gene="queA"
                     /locus_tag="OO568_001505"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6640907.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            22275..22848
                     /gene="acpH"
                     /locus_tag="OO568_001506"
                     /pseudo
     CDS             22275..22848
                     /gene="acpH"
                     /locus_tag="OO568_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(22853..24670)
                     /gene="malZ"
                     /locus_tag="OO568_001507"
     CDS             complement(22853..24670)
                     /gene="malZ"
                     /locus_tag="OO568_001507"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6640908.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYLFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQSEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPSCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(24826..26199)
                     /gene="proY"
                     /locus_tag="OO568_001508"
     CDS             complement(24826..26199)
                     /gene="proY"
                     /locus_tag="OO568_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6640909.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(26275..27594)
                     /gene="brnQ"
                     /locus_tag="OO568_001509"
     CDS             complement(26275..27594)
                     /gene="brnQ"
                     /locus_tag="OO568_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6640910.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(28001..29296)
                     /gene="phoR"
                     /locus_tag="OO568_001510"
     CDS             complement(28001..29296)
                     /gene="phoR"
                     /locus_tag="OO568_001510"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6640911.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVFNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEASPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(29354..30043)
                     /gene="phoB"
                     /locus_tag="OO568_001511"
     CDS             complement(29354..30043)
                     /gene="phoB"
                     /locus_tag="OO568_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6640912.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLLGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            30233..31435
                     /gene="sbcD"
                     /locus_tag="OO568_001512"
     CDS             30233..31435
                     /gene="sbcD"
                     /locus_tag="OO568_001512"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6640913.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            31432..34575
                     /gene="sbcC"
                     /locus_tag="OO568_001513"
     CDS             31432..34575
                     /gene="sbcC"
                     /locus_tag="OO568_001513"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6640914.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADMKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVELDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTTLQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34701..35882
                     /gene="araJ"
                     /locus_tag="OO568_001514"
     CDS             34701..35882
                     /gene="araJ"
                     /locus_tag="OO568_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6640915.1"
                     /translation="MKKVILSLALGTFGLGIAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYLLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALFSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(36025..36933)
                     /gene="mak"
                     /locus_tag="OO568_001515"
     CDS             complement(36025..36933)
                     /gene="mak"
                     /locus_tag="OO568_001515"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6640916.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            37058..37969
                     /gene="rdgC"
                     /locus_tag="OO568_001516"
     CDS             37058..37969
                     /gene="rdgC"
                     /locus_tag="OO568_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6640917.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCSSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELHDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(38047..38502)
                     /locus_tag="OO568_001517"
     CDS             complement(38047..38502)
                     /locus_tag="OO568_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640918.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAAGRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(38499..>38773)
                     /locus_tag="OO568_001518"
     CDS             complement(38499..>38773)
                     /locus_tag="OO568_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HCR6640919.1"
                     /translation="SRNDHLRQTALRNAHTPASLLTTLTEPQDRSLAINNPQLAADVK
                     TAWLKEDPSLLLFVEQPDLSQLRDLVKTGATRKIRSEARHRLEEKQ"
BASE COUNT         9855 a        10272 c         9663 g         8983 t
ORIGIN      
        1 acctagcata tctgcaacaa gctgcaacac tgggcagtag taatgcccag gcggaaattg
       61 gctatttata tctcatcgga aaagaactac cacagaatct tcctgatgct ggtgtctggt
      121 tcaaaaaagc agctgcacaa ggtaattttg ccgcacattt ctatctggga aggatgtatc
      181 agaatggtga cggcgttgag agaaatatgg aaaaagccag gtttcatcta tctaatgcgg
      241 cagaaggtgg aatagaacct gcgcttaaag cattaaatga acttgaacat caaacaaaat
      301 aaagcgctca gggaccacgt aaggttcatc aaacagaaaa ggatatagtt tgcgatgaaa
      361 gcgaaatcta ctttgttatt aatgatttta tctgtttttt attctcatca ggttttatcc
      421 agcgacccca ctcagccgga tgtttattct gtcgtcgaga aaaaactaaa ctctgcttta
      481 cctctggaag aaaataccac cttcagatcg caggctgaat ggtttaattt gcaatatgaa
      541 ttattagtat ctggctaccc tgaacgtgct tacaatttac tgagcaaact tgagacagaa
      601 tcgaaaaacg ctaaatatta tctggattta actgcctcca gagtcgctga agaagaagca
      661 ccagagacag ccttaacttt tttatcaaaa attgggttaa ataaaccggc tactcttaca
      721 tcctttgaga gctatatcaa tagctggata aaaagcaaac aacccgaaaa ggctatgcag
      781 cttctcagcc tcgatgagaa tgccctgaat tattatttac cgactgtgtt agtcgcatat
      841 cagatcgttc ctgataaggc aataagtatt tataattctg tatatggaga taatgttgtt
      901 ataccctatc agcaagtcac catgttactt acagttgcgg agggttatca aacaaaaggc
      961 gatataaaaa acgccattca ttatgccgat aaggcgctga atatgtttga taaagctatt
     1021 gctgtagata gcagtcgtga atcccattat agtgatgagt atttgaaact catgaatatc
     1081 tacgccgcca atggcaacaa agaaaaagcg gtggttttat ctcaacgtct acacaaagcc
     1141 attaccgaat ctcaatcgaa ttacttaagt actctggaag gaattttatt gttttataga
     1201 aataatgata tgcagcaaga ctatcaaaac agcctttcaa attacatagt atttatcgat
     1261 gaaatttttg ccttttcccc atcaacaaga agtgaattaa gcctcattaa tttattaaat
     1321 tcattaaatg aggtggaatt gatgagaaaa cgtctggcgt ttctgatgac agcaccagaa
     1381 tacgcttgtt atgatagtca gtattgttat gaagacaaag tcagcgcatt aaaaatcctg
     1441 tatcgtcatc atgacaatga gctaatggac aaatatttca cgactcttct taaagagatc
     1501 agtaatcaac cattagaaga ctgggatata ataataagta atactggtga aaaatttagt
     1561 gaagtgggtc taaccaaata tgctcaacat ctcgcatctt tcgctgaaga attttatata
     1621 aaagagcaaa aatcacattc tgaaagtgaa atgagaggac tattttcatc acttgccgaa
     1681 ttatatagtt ttggtaatga tactgccagc gcaaaccgag tgttccatca gcatgtccct
     1741 gccttcaata cagatcatat gattcaatat tttataaatg ccaaagaatg gaacaatgcc
     1801 agggaattat tgataaaaga ggaaatgttg ggtatgcata atctgatatt gttggagaat
     1861 atttgtatgc aaaaaaatgc agaatgcatg gcacacatta cctttacgct taataaatta
     1921 acaacacaac cagcaataac aaaaatagat agcgtgggta atgagcaact ttatcagatt
     1981 ggcaaaatct accataagtt ggaaattaaa cctgagccag agcaacaggt tttaattcag
     2041 tcgctttatg accgggcatc aggaagcgcc tctgcaactc aataaatgca tggtcaaaag
     2101 ggccaccacg gtggccctga caatcagtag ctatatccag aaagttaatc gcggaagttt
     2161 ttgaactgga acggctgacc gagatcgcca ccgcgcacca tcgccatgac agcctgcaaa
     2221 tcatcacgag atttgccagt cacgcggatc tcatcgccct gaatttgcgc ctgcactttc
     2281 agtttgctgt ctttgatcat cttgacgatt tttttctgag tggcgctctc aatgccctgt
     2341 ttcagtttcg cttccacaaa ccaggtttta ccgctatgaa cgatattttc cggtacatcc
     2401 agtgaactgc cttcaatgcc gcgcttcagc agcttggcac gcagaatatc cagcaactga
     2461 ttgacctgga agtcggactc gctcaacact ttgatggttt tgctggcgtc gttcagctca
     2521 aatgaggctt caacgttacg gaagtcaaaa cgggactcca cttcgcgact cgcgttatcg
     2581 accgcgttac gtgcttcctg aagatcaact tcagagacaa tatcgaaaga tggcatcttt
     2641 tcctctccct tcatttttga tgcgaagcat aatacctgca aagttaaata accaacagtc
     2701 ctgcaccagg acgtatccct taattgtgca tagtcgcaac agcatgcaca attaagggat
     2761 agccttattg aacacccgga gtggttgtgg gtgaggagga acaatgaaaa ttaccgtatt
     2821 gggatgcggt gccttagggc aattatggct tacagcactt tgtaaacagg gtcatgaagt
     2881 tcagggctgg ctgcgcgtac cgcaacctta ttgtagtgtg aatctggttg agacagatgg
     2941 ttcgatattt aacgaatcgc tgaccgccaa cgatcccgat tttctcgcca ccagcgatct
     3001 gctcctggtg acgctgaaag catggcaggt ttccgatgcc gtcaaaagcc tcgcatccac
     3061 actgcctgta actacgccaa tactgttaat tcacaacggc atgggcacca tcgaagaatt
     3121 gcaaaacatt cagcagccat tactgatggg caccaccacc catgccgccc gccgcgacgg
     3181 caatgtcatt attcatgtgg caaacggaat cacgcatatc ggtccggcac ggcaacagga
     3241 tggcgattac agttatctgg cggatatttt gcaaaccgtg ttgcctgacg ttgcctggca
     3301 taacaatatt cgcgccgagc tgtggcgcaa gctggccgtc aactgcgtga ttaatccact
     3361 gactgccatc tggaattgcc cgaacggtga attacgtcat catccgcaag aaattatgca
     3421 aatatgcgaa gaagtcgcgg cagtgatcga acgcgaaggg catcatactt cagcggaaga
     3481 tttgcgtgat tacgtgatgc aggtgattga tgccacagcg gaaaatatct cgtcgatgtt
     3541 gcaggatatc cgcgcgctgc gccacaccga aatcgactat atcaatggtt ttctcttacg
     3601 ccgcgcccgc acgcatggga ttgccgtacc ggaaaacacc cgcctgtttg aaatggtaaa
     3661 aagaaaggag agtgaatatg agcgcatcgg cactggtttg cctcgcccct ggtagtgaag
     3721 agactgaagc cgtcaccacg atcgacctct tggttcgcgg cggtatcaaa gtcaccactg
     3781 ccagcgtcga cagcgatggc aacctggcaa ttacctgctc gcgcggcgtg aagctgctgg
     3841 cggatgcgcc gctggtcgaa gtggctgatg gcgaatatga cgtgatcgtg ctgcctggtg
     3901 gcattaaagg cacggagtgt tttcgcgata gcactctgct ggttgaaacc gttaaacagt
     3961 tccaccgttc cgggcgtatc gtcgcggcta tttgcgccgc gccagccacc gtgctggtgc
     4021 cgcacgatat ctttccgatt ggcaatatga ccggcttccc gacgctgaaa gacaaaattc
     4081 ccgctgaaca atggcaggac aagcgcgtcg tctgggatgc acgggtaaaa ttgctgacca
     4141 gccaggggcc gggtacagct atcgactttg gtctgaaaat tatcgacctg ctggttgggc
     4201 gcgagaaagc ccatgaagtg gcatcacaac tggtgatggc ggcagggatt tataattatt
     4261 acgagtagtg tcggatgcgg caaacgtcgc atctgaccag atgcgacgta aaaactacaa
     4321 attacgggcg atataccttc acattgttaa agccttgctc gcgcagatag agcgcctgca
     4381 gacggctcat caccccgcgc tcacaccaca gcagccaggt tttgttctgg tcgagatcgc
     4441 caaatttggt gctcagttta tagaacggca gagaaaccac atcaatccct tcgactttca
     4501 gtggcttatc ttcctgttca tcgacagaac ggatatcgag aatcacgtcg ttcgggccga
     4561 agccgttgac ggtttccact tccaccactt cctgctcggt ctgctgggcg atttcgcgga
     4621 tatcaacgtt attcgcttcc tcaaccactt tatcgagaat actgaagtcg aacttctctt
     4681 cttccgcttc aatcttcgat ttaaccgctt tcaccgtcgg gcttttggag atcacgccac
     4741 aatattccgg catcgtgcga gcaaagtctt cggtgccaat ctggcgagcc aggttgatga
     4801 tgtgctcttt gtcgtaagaa atcagcggac gcaggatcag cgtgtcagag acgttatcaa
     4861 tcaggcgcag gttggtcagc gtctgactgg acacctggcc gagcgcttcg ccggtgacca
     4921 gcgcctgtac gccgtaacgt tcagccactt tagatgcggc acgcaccatc atacgtttga
     4981 ggataacgcc catctgaccg tcgtcgattt tctcgagaat ttccccgaca accggttcga
     5041 aattaatggc gacaaaacgc acgcggtggg agctgccaaa gcggttccac agataatgcg
     5101 ccacctgacg cacgccaatt tcatgcgctg cgccgccgag gttaaagaag cagtaatgca
     5161 cgcggcagcc gcgacgcatc aacatataac tggaaacacc ggagtcgaaa ccaccggaaa
     5221 tgagcgacag cacatcttcc tgggtgccaa tcgggaaacc accaatacct tcgtagcgac
     5281 ctttaatcag caggaggcga tcgtcttcca cttccagatg aacagttaca tccgggttag
     5341 tcagcttcac gcgcgcggat tcaatatgct gatttaaacc gccgccgacg taacgttcca
     5401 catcaatcga gctaaaatca tgtttgccac ggcgcttcac gcgtacgcag aaggttttgc
     5461 cttccagctg atcgcgatac tgaaccaacg ctttctcaaa aatatcgtgc atgtcggtaa
     5521 acggcacgtc ttcgacttcg agaatatggt ggatacccgg aatacgggtc agggcgtcgc
     5581 gaatagtcag acgctggttt tcatctttcg cgcgaacttc gatgttatcc cagtggcgga
     5641 cgacagcgag cgtctcatca tagtgcttta aaacgttacg aatgttcccg gtaaggattt
     5701 ttataaagcg caagcgcaca gattggcttt tgatggtgat ttccgggaac aatttaatga
     5761 taaacttcat ggcggcaatg gttcgttggc aagccttaag cgacttgtat agggaaaaat
     5821 acagcagccc acacctgcgg ctgcatccag gcgcggaagt ataccactaa catcgctttg
     5881 ctgcgcacat caccttacca ttgcgcgtta tttgctattt gccctgagtc cgttaccatg
     5941 acggggcgaa aaatattgag agtcagacat tcattatgcc gaagaaaaat gaggcgcccg
     6001 ccagctttga aaaggcgctg agcgagctgg aacagattgt aacccgtctg gaaagtggcg
     6061 acctgccgct ggaagaggcg ctgaacgagt tcgaacgcgg cgtgcagctg gcacgtcagg
     6121 ggcaggccaa attacaacaa gccgaacagc gcgtacaaat tctgctgtct gacaatgaag
     6181 acgcctctct aacccctttt acaccggaca atgagtaatg gactttccgc agcaattcga
     6241 agcctgcgtt aagcaggcca accaggcgct gagccgtttt atcgccccac tgccctttca
     6301 gaacactccc gtggtcgaaa ccatgcagta tggcgcatta ttaggtggta agcgcctgcg
     6361 acctttcctg gtttatgcca ccggtcatat gtttggcgtt agcacaaaca cgctggacgc
     6421 acccgctgct gccgtagagt gtatccacgc ttactcatta attcatgatg atttaccggc
     6481 gatggatgat gacgatctgc gtcgcggttt gccgacctgc catgtgaagt ttggcgaagc
     6541 aaacgcgatt ctcgctggcg acgctttaca aacgctggcg ttctcgattc taagcgatgc
     6601 caatatgccg gaagtgtcgg atcgcgacag aatttcgatg atttctgaac tggcgagcgc
     6661 cagcggtatt gccggaatgt gcggtggtca ggcattagat ttagacgcgg aaggcaaaca
     6721 cgtacctctg gacgcgcttg agcgtattca tcgtcataaa accggcgcat tgattcgcgc
     6781 cgccgttcgc cttggtgcat taagcgccgg agataaaggg cgtcgtgctc tgccagtact
     6841 cgacaagtac gcagaaagca tcggccttgc cttccaggtt caggatgaca tcctggatgt
     6901 ggtaggagat actgcaacgt tgggaaaacg ccagggtgcc gaccagcaac ttggtaaaag
     6961 tacctaccct gcacttctgg gtcttgagca agcccggaag aaagcccggg atctgatcga
     7021 cgatgcccgt cagtcgctga aacaactggc tgaacagtca ctcgatacct cggcactgga
     7081 agcgctagcg gactacatca tccagcgtaa taaataaaca ataagtatta ataggcccct
     7141 gatgagtttt gatattgcca aatacccgac cctggcactg gtcgactcca cccaggagtt
     7201 acgactgttg ccgaaagaga gtttaccgaa actctgcgac gaactgcgcc gctatttact
     7261 cgacagcgtg agccgttcca gcgggcactt cgcctccggg ctgggcacgg tcgaactgac
     7321 cgtggcgctg cactatgtct acaacacccc atttgaccaa ttgatttggg atgtgggaca
     7381 tcaggcttat ccgcataaaa ttttgaccgg acgccgcgac aaaatcggca ccatccgtca
     7441 gaaaggcggt ctgcacccgt tcccgtggcg cggcgaaagc gaatatgacg tattaagcgt
     7501 cgggcattca tcaacctcca tcagtgccgg aattggtatt gcggttgctg ccgagaaaga
     7561 aggcaaaaat cgccgcacca tctgtgtgat tggcgacggc gcgattaccg ctggcatggc
     7621 gtttgaagcg atgaatcacg cgggcgatat tcgtcctgat atgctggtgg tcctcaacga
     7681 caatgaaatg tcgatttccg aaaatgttgg cgcgcttaac aaccatctgg ctcagctgct
     7741 ttccggtaag ctttactctt cactgcgcga aggcgggaaa aaagttttct ctggcgtgcc
     7801 gcccattaaa gagctgctca aacgtaccga agaacatatt aaaggcatgg tggtgcctgg
     7861 cacgttgttt gaagagctgg gatttaacta catcggcccg gtggacggtc acgatgtgct
     7921 ggggcttatc accacgctaa agaacatgcg cgacctgaaa ggcccgcagt tcctgcatat
     7981 catgaccaaa aaaggtcgtg gttatgaacc ggcagaaaaa gacccgatca ctttccacgc
     8041 cgtgcctaaa tttgatccct ccagcggttg tttgccgaaa agtagcggcg gtttgccgag
     8101 ctattcaaaa atctttggcg actggttgtg cgaaacggca gcgaaagaca acaagctgat
     8161 ggcgattact ccggcgatgc gtgaaggttc cggcatggtc gagttttcac gtaaattccc
     8221 ggatcgttac ttcgacgtgg ctattgccga gcaacacgca gtgacctttg ccgccggtct
     8281 ggcgattggt ggatacaaac ccattgtcgc gatctactcc accttcctgc aacgcgccta
     8341 tgatcaggtg ctgcatgacg tggcaattca aaagctaccg gtcctgttcg ccattgaccg
     8401 cgcgggcatt gttggtgctg acggtcaaac tcaccagggc gcttttgatc tctcttacct
     8461 gcgctgcata ccagaaatgg tcattatgac cccgagcgat gaaaacgaat gtcgccagat
     8521 gctctatacc ggctatcact ataacgatgg cccgtccgcg gtgcgctacc cgcgcggcaa
     8581 cgcggttggc gtggaactga cgccgctgga aaaactacca attggcaaag gcattgtgaa
     8641 gcgtcgtggc gagaaactgg tgatccttaa ctttggtacg ctgatgccag aagcggccaa
     8701 agtcgccgaa tcgctgaacg ctacgctggt cgatatgcgt tttgtgaaac cacttgatga
     8761 agcgttaatt ctggaaatgg ccgccagcca tgaagcgctg gtcaccgtag aagaaaacgc
     8821 cattatgggc ggcgcaggta gcggagtgaa cgaagtgctg atggcccatc gtaaaccagt
     8881 acccgtgctg aacattggcc tgccggactt ctttattccg caaggaactc aggaagaaat
     8941 gcgcgccgaa ctcggcctcg atgccgccgg tatggaagcc aaaatcaagg cctggctggc
     9001 ataatcccta ctccactcct gctatgctta agaaattatt catggcagga gtggcagcat
     9061 gcaatacaac cccttaggaa aaaccgacct tcgcgtttcc cgactttgcc tcggctgtat
     9121 gacctttggc gagccagatc gcggtaatca cgcatggaca ctgccggaag agagcagccg
     9181 tcccataatt aaacgcgcgc tggaaggtgg cataaatttc tttgataccg ccaatagcta
     9241 ttccgatggt agcagcgaag agatcgttgg tcgcgcactg cgggatttcg cccgccgtga
     9301 agacgtagtc gttgccacca aagtgttcca tcgcgttggt gatttaccgg aaggattatc
     9361 ccgtgcacaa attttgcgct ctatcaacga cagcctgcga cgtctcggca tggattatgt
     9421 cgatatcctg caaattcatc gcggggatta caacacgccg atcgaagaga cgctggaagc
     9481 cctgaacgac gtggtaaaag ccgggaaagc gcgttatatc ggcgcgtcat ccatgcacgc
     9541 ttcgcagttt gctcaggcgc tggaactaca aaaacagcac tgctgggcgc agtttgtcag
     9601 tatgcaggat cactataatc tgatttatcg cgaagaagag cgcgagatgc tgcctctgtg
     9661 ttatcaggag ggcgtggcgg tgattccgtg gagcccgatg gcgcgggggc gactgacgcg
     9721 tccatgggga gaaactaccg cacgactggt gtctgatgag gtggggaaaa atctctataa
     9781 agaaagcgat gaaaatgacg cgcagatcgc agagcggtta acgggcgtca gtgaagaact
     9841 cggtgcaaca cgagcacaag ttgcgctggc ctggttgtta agtaaaccgg gcattgccgc
     9901 accgattatc ggaacttcgc gggaagaaca gcttgatgag ctgctgaacg cggtggatat
     9961 cactttgaag ccagaacaga ttgccgagct ggaaacgccg tataaaccgc atgcggtcgt
    10021 aggatttaaa taagagtcca ggcctgatga gacgtgacaa gcgtcacatc aggcatcggt
    10081 gcacaactac gacagaatac ccagcggcca gtgatgaccg ataaagtaca ggatgcctgc
    10141 ggaaatcacc ccggcgacaa tatcgtcgat catgatcccc atgccgccat gtacattgcg
    10201 atcaaaccag cggatcggcc acggcttcca catatccaga atacggaaaa tcacaaaccc
    10261 ggcggcaacc cactgccagt cattggtcgg cagcgccatg agcgtgatcc acataccaat
    10321 aaattcgtcc cagacaatgc tgccgtgatc gtgcacgccc atatctttcg ccgtttgatg
    10381 acaaagatag acgccgatac agatccccaa catcaccacc agcgagtaga gctgccaggg
    10441 taaaaaggtc atcagatacc agaaagggat cgccgccagc gatcccatcg tgccaggaac
    10501 gattgggctt aatccacttc cgaatccgac agcaagcaga tgccacggat tactcatctt
    10561 caggcgactt ttcgcgacat ctttatggcg tgacaaaatg gtcatatcct ttccagtcaa
    10621 acgtgacagg ttcgccgtca cgaataaaac aaagcccttc gatatcggcg gtcatttgcc
    10681 cgatacaggt aaacggtacg ccaaggtgtc cgagagcaac atccagcgcg ccacggttca
    10741 gttccggcac cgtgaaacac aactcgtaat cttcaccgcc agagagcgcc cagcgcagcg
    10801 cctgttccgg ttcaacatgg cgagaaagcg catcagaaaa cggcagcaat gccaggtcaa
    10861 tacgtgcgcc gcagtcgctg gctttcacga tatgcccgag atcggaaatc agaccgtcag
    10921 agagatcgat agctgaattt gccagatcgc gcagtgcctg cccctgtaaa atacgcggcg
    10981 atggacggag atgacgtttg atcaagtagt cagcatcttt agcatcggca acctgcaaac
    11041 ggttttgcaa aatcgccagc ccggcggcgc tatcgcccgg tgtaccggtc acatagatcc
    11101 agtcacccgg tttcgcccca gagcgcgtta aggctcgtcc catcggaaca aagccgtgga
    11161 tacccaacgt cattgataat ggcccacgcg tggtatcgcc gccaatgagt tgcatatcgt
    11221 aataattgag aagatcaaac aaactgtcgc tgaaggactc aagccacgct tcgtctacgt
    11281 ccggtaaggt taatgccagc gtcagccagg ccggatcggc ccccatcgct gccagatcgc
    11341 ttaggttcac cgccagtgct ttataagcca gatcagcagg atcgatatca gggaggaaat
    11401 ggttgcccgc caccagcgta tcagtgctga tcgccagggt ctgtttctcg gggatattga
    11461 gaagagcgca gtcgtcgccg atgcccagtt cgacatcaag acgagaactt cttacacggt
    11521 caaaataacg ggcaatcagg gagaactcgc cacatgccat acgttatgcc tcagcaggta
    11581 aaaaagaaaa ggccggcgat catggaacag tcttccgtga atctaccggc ctggatatta
    11641 ctttttgtta gggcgaatca caggtgctgc tttatcgagt acgccgttga cgaacttatg
    11701 gctgtcttct gcgccgaacg atttcgccag ttcgatcgct tcgttaatgg ccactttgta
    11761 tggcacatca ctacgtttag acagttcgta cagcgcaatg cgcagtactg ctttttctac
    11821 ctggcccagt tcttccagca ggcgggacag gtagggcttc atcagtccgt cgaggtatgc
    11881 ggtgttagtc gccaccccgg ccagcagctc acggaagtac aggacgtcaa cgtcttttac
    11941 atcctgttca gccaggaact ggtattcaac atcagcgatg tcgttctggg acaactgcca
    12001 ggagtagagc gcctggacgg cacactcacg agcgcggcga cgagcagcag gtttcacgga
    12061 tttcccctta ctaatttcag gccttgatgg ctttcaatac attaatcatt tcaagcgcgg
    12121 tcagtgcagc ttctgcacct ttgttgccag ctttggtgcc agcacgttcg atcgcttgtt
    12181 caatgctttc agtggtcaga accccaaaag caaccggaat ttcgctgtcc tgggcaacat
    12241 gcgccaggcc gttgcttgca ccaccagcga catattcaaa gtgggcagtg ccaccacgaa
    12301 taaccgtacc cagcgcaatc accgcgtcgt atttaccggt tttagccagt gcacccgccg
    12361 ccagcggcag ctcataggca ccaggcaccc aaacaacggt aatgttttca tcttttacct
    12421 gaccgatacg tttcagtgcg tcaattgcac cttccagcag gctgtcattg ataaagttgt
    12481 tgaaacgcgc gatggtgatg gcgacgcgag cgtccggggt agcaacgtta gcttcaataa
    12541 tgttcatttt cttccttcgg gttcgagtat ggccccgcag gggggcggat tttagcataa
    12601 tatttcgtgc gctgcttccc tttcgagccg ggagagcatg cccccactaa atgcaggcaa
    12661 acatccgggc ctacatgacg tatctctttg aatttaaatt ggggggcgtc ggctaatttc
    12721 tcaagccctg gcagcgagca taatccacgg gcgtcgctgc ctaatagttt aggtgcgata
    12781 tagacaatca gttcatcgac taaacccgcc tgtagcaata cgccagcgag cgttggcccc
    12841 gcttcccccc agatgctgtt aatctgctgt ttacccagtt gcatcatcag tacaaccaga
    12901 tccagatgac ctttatgctc tggaatcagc aaggtacgca ccgtttccgg ccactcacga
    12961 gaatcttcct gcgtacgcgc gaaccaggtt tcgccgggct gctgcacaat gcgatgttcc
    13021 ggcgtcacgc gattttggct atcaatcaca atacgtactg gctggcggag attttgttgc
    13081 gggtagagcg cctgagtttg ttcatccagt tcagaccaac gcactgttaa ggctggatca
    13141 tccgccagca ccgtggcgct gctggttaaa atggcatgac tttgtgcgcg ctgtcgttgt
    13201 acgtcgcgcc gcgcctgagg cgaagtgatc cactggcttt cgccgctcgc catcgccgtg
    13261 cagccatcaa gcgatgcgcc aagttttaac tgaatataag gaaagccggt gcgcatccgc
    13321 ttgagaaagc ctttattcaa ttgctcggct tcactcatca tcagcccgtg gctgacgtca
    13381 atgccagcct gttgcagacg gtaaagtcca cgcccagcga cctgcgggtt aggatcttgc
    13441 atcgctgcga ccacgcgcgc tacgccagcg gcgatgagtg cgtcacagca cggtggcgta
    13501 cggccatgat ggctacaggg ttcgagtgtc acatacgccg ttgcgccttt ggctttttca
    13561 cccgccatac gcaacgcgtg tacttcggca tgtggttcac ccgctcgttg gtggtaacct
    13621 tcaccgacaa tttcgccatc tttgacaatg acgcacccga cattcgggtt ggggtgggtg
    13681 gtaaaacgtc ctcgttgcgc cagctttagc gcccgcgcca tgtaatactc gtcctgcacg
    13741 gcttagtcct ccaggcgcgc gatctcttcg ccaaattctt tgatatcttc gaaactgcgg
    13801 tagacagagg caaaacggat ataggcgact ttatcgagct ttttcaattg ctccatcacc
    13861 agattgccaa tcatcttgct cggtacttcg cgctcaccgg tggcgcgcag ctgcgattta
    13921 atatgattga ttgccatttc aacgtaatcg gaactcaccg gacgtttttc cagcgcccgc
    13981 agcattccgc tacgcaattt ctcttcatta aacggttcac gcacgtcgtt gctttttaca
    14041 acacgcggca taaccagctc cgccacttca aaggtggtga aacgttcatt acacaccaga
    14101 cactgccgac ggcggcgtac ggatgaaccc tcgcccacga gacgagagtc aattacctta
    14161 gtgtccacgg cgaaacagaa tgggcaatgc atacggcgtc ctgaccaggt ggttaacgga
    14221 aatctatttt accctgaact gacgatacaa caaaggcgca acgcttttga gataaaggat
    14281 cgatgctttc gctgctttta ccctctacca ttaggctaat tgcgacgaga aagggacgac
    14341 ctatgaatac taacgttttt cgactgctcc tgctgggaag cctgttcagc cttagcgcct
    14401 gtgtgcagca aagtgaagtg cgacagatga aacacagcgt cagcacgctg aaccaggaga
    14461 tgacgcaact caatcaagaa acagtcaaaa tcactcagca aaacaggctg aatgcaaaat
    14521 ccagcagtgg ggtttacctt ctgcctggag cgaaaacacc ggcaagactg gaaagccaga
    14581 tcggtacttt acgtatgtcg ctggtgaata ttacgcctga tacagatggc accaccctta
    14641 cactacgtat tcagggtgag tcgaatgatc ccttgcccgc attcagcgga actgttgaat
    14701 atgggcaaat tcagggaaca atagacaact ttcaggaaat caatgtgcag aatcaattga
    14761 ttaatgcccc tgccagcgtc ctcgccccca gcgatgttga tattccgtta caattaaagg
    14821 ggatttctgt ggatcagtta ggctttgtac gtatccacga cattcaacct gttatgcagt
    14881 aaacgtattt cgggacgatt ttgtgcctcc cgcaacatct ttccccgtca ttttgttact
    14941 ctgcttacat cacttggatt gatagtaaaa gtttgcaaca agggcgaaag tcagtacaat
    15001 ccccgcccga atgtgtgtaa acgtgaacgc aatcgattac gtaaatgata gaactgtgaa
    15061 acgaaacata tttttgtgag caatgatttt tataataggc tcctctgtat acgaaatatt
    15121 tagaaacgca atttgcgcct ttttcactcc cgcaagggat tttcaaacag tggcatacat
    15181 atgaaaaaaa cattactggc agccggtgcg gtactggcgc tctcttcgtc ttttactgtc
    15241 aacgcagctg aaaacgacaa accgcagtat ctttccgact ggtggcacca gagcgttaac
    15301 gttgtcggaa gctatcacac ccgtttcgga ccgcagatcc gcaacgatac ctaccttgag
    15361 tacgaagcat tcgctaaaaa agactggttc gacttctatg gttatgcgga tgcgccggta
    15421 ttcttcggcg gtaactccga tgctaaaggt atctggaacc acggttctcc gctgtttatg
    15481 gaaatcgaac cacgtttctc catcgacaag ctgaccaata ctgaccttag cttcggtccg
    15541 ttcaaagagt ggtacttcgc gaacaactac atttacgaca tgggtcgtaa taaagatggt
    15601 cgccagagca cctggtacat gggtctgggt accgatatcg acactggcct gccgatgagc
    15661 ctgtccatga acgtctatgc gaaataccag tggcagaact atggcgcagc gaacgaaaac
    15721 gagtgggacg gttaccgttt caaaattaaa tactttgtgc cgattaccga tctgtggggc
    15781 ggtcagctga gctacatcgg cttcaccaac ttcgactggg gttccgattt aggggatgac
    15841 agcggtaacg caatcaacgg tattaagatc cgtactaata actctatcgc ttccagccat
    15901 attctggctc tgaactacga tcactggcac tactctgtcg tagctcgtta ctggcacgac
    15961 ggtggtcagt ggaacgacga tgcagaactg aacttcggca acggcaactt caacgttcgc
    16021 tctaccggct ggggtggtta cctggtagta ggttacaact tctgattatg aaaatgccgg
    16081 gatttattcc cggcatttct gattgttaac aacgtgtgct ttgttcatgc cggatgcggc
    16141 gtaaacgctt tgtccggccc acaaaagcac gtaaattcaa tatattgctg aaataacgta
    16201 ggcctgataa gcgtagcgca tcaggcaatt ttacgtttta atcacttctt cttgttcatc
    16261 atcgctttca ggtcagcgaa tgggttgtac ttcgcttcac cgacatcttt ctgcgcatct
    16321 tcccctgcga taacggtcgt accatactga tccgcttcgg tatatttcga atgctcatga
    16381 tcgtggcaat agagacacaa caattcccag ttactgccat cttccgggtt attggtatgg
    16441 tcgtgatcaa tgtggtgaac ggtaagttca cgcaggttag aataaacaaa ctcgcgggaa
    16501 cagcgaccgc agacccacgg atagattttt aatgcttttt cgcgatagcc gctttctaac
    16561 cgtgcatagt tttttgggat gatagccatg aaaagttacc tggatgaaat gtagggttac
    16621 gcgcagtttc ccaagatgcg gggattttcg caatctccat acatcaaaaa aaatcccgat
    16681 cttctgaccg ggattttcaa catcaacggg aacttgatta aggcagaatt gacggctgat
    16741 ccgccccttc tttttccact ttctgctgca acatgtgttc gcgcttcata cccagtttca
    16801 gagccaacgc agatgctacg tagatggaag atgcagtgcc gatggaaaca ccgataagca
    16861 tggtcagcga gaagccttcc agtaccggac caccgaagag atacagcatc aggataacca
    16921 tcaaggtagt accggatgtg atcaaggtac ggtgcagcgt ctgggtcaag gacacgttaa
    16981 agatttcgta aggcgtaccg cgacggatct tgcggaagtt ttcacgaatt cggtccgata
    17041 ccacgatact gtcgttaagc gagtaaccga taaccgacat caacgatgcc acaatggtca
    17101 ggtcaatctc gatatggaat aacgacaaaa tacccagcgt aatgataacg tcgtgcgcca
    17161 gcgcaataac cacccctgcc gccagtcgcc actcaaagcg gaaacctacg tacacgagga
    17221 tagacagcag cgctgccatc aacgccatcg caccggtttg cgcaaggtct gcccccacgc
    17281 tcggaccgac gaactcaata cgcttcactg ctgcattctg attggtggat tcgttaatca
    17341 ccttcagaac ttggctgccc agcacctgac cgccggtttc gccttcagca ggcggcatac
    17401 ggaccatgat gtcgtggctg ctaccaaagt tttgcagcat tggctcttca aaaccggctt
    17461 tttgcaatgc atcacgcatt acgtcaattt cagccggttt ttcgagcgta atttcgataa
    17521 ccgtaccacc ggtgaaatcc agcccccagt taaagccgcg cacgcccata ataacgatag
    17581 cagcgattaa cagcagacca gagatgccga aagcccagta gtcccagcgc ataaagtcat
    17641 agactttacg gccgtggttt agttgttcaa cagtatattc ctgtgccaca tcgcactcct
    17701 cagattgaca gcttcttgac gcgcttgccg ccatatagca ggtttacgat ggcacgcgta
    17761 ccgacaatcg cggtaaacat cgacgtcgcc acaccgatac cggtagtaat cgcgaaccct
    17821 ttaattgccc cggtacccac tgcgtacagg atgatgactt taatcagcgt ggtgatgttc
    17881 gcatcgaaga tagaactgaa tgcgccacga taaccttcat caattgcctg ttgaacagta
    17941 cgtccgttgc tcaactcttc tttaatacgt tcgttgatca gtacgttcgc atcgaccgct
    18001 accgcaaggg ttaagacgat acccgcgata cctggcatac tcagtgttgc gcctggcaac
    18061 agcgacataa taccgacgat caagatcaag ttggcaatca gagcactggt cgcaatcaga
    18121 ccaaacttct tatagaagat gatcatgaac agaatagaaa ccagcagacc ggcaaggcaa
    18181 gcctccagcc cctgctcaat gttctgcata cccagggtcg ggccaattgt acgttcttca
    18241 acaatctgaa tcggcgcgat caacgcaccg gcacgcagca gcagtgacag ctgacgggct
    18301 tcgttcgggt tgttgatgcc ggtgatacgg aagctgttac ccagacgaga ctggatgttg
    18361 gcgatgttaa tcacctcttc ctgtttcacc agaaccgcac gaccatttgc atctttctta
    18421 ccgctgtctt tgtactccac aaacagggtt gccatcggtt tgccgatgtt gtccttggtg
    18481 aagttagaca taatgttgcc accagcgcta tcgagcgaga tgttaacctg cggctggttg
    18541 tattcgtcct ggctggaagt ggagtcggtg atatggtcac cagtcagaat tacgcgtttg
    18601 tacagcacaa ccggctgacc ttcgcgggtc tgtttcactt cagagtcgcc cggtacgcga
    18661 ccggatgccg cagcggcctg atcaacattg gtgtttacca gacggaattc cagcgttgcc
    18721 gtcgcgccca gaatctcttt cgcacgcgca gtgtcctgaa taccaggcag ttcaacaacg
    18781 atacggtcag caccctgacg ctgaaccacc ggctccgcca cgccaagttg gtttacacgg
    18841 ttacgcagga tattaatgtt ctgctgcacc gcatattcac gcgcttcact cagacgagca
    18901 tcgctcatta ccgcacgcag ctggttgctg ccctggctgc taatcaccag gtccggatgg
    18961 cgcttgctca gatacgcaat ggcttcatca cgagctttag catcgcggaa agtgatgctc
    19021 aggccgtagt tgttttcttt acgaacagtg gtatacggga tgcccttttc gcgcaggtca
    19081 ctgcgtaggc tatcgatatt ttgttcctgg agtttgccaa gcgcggtgtc catatccact
    19141 tccatcagga agtgaacgcc gccacgcagg tcaaggccga gcttcatcgg ctcagcatga
    19201 atagctgcca gccagcgcgg cgttgccggg gcaaggttaa gcgccacgac gtatttgtca
    19261 cccataacgc ccattaatgc ttcacgagcg cgcaactggg tgtcagtgga gtcgaagcgc
    19321 gcaagaatag cgccctcttc cagtgccaca gacttagcag ttattttttc ttcttgtaac
    19381 gttttctgga cctggatcag cgtttgctca ctggcggcga ctccgcgcgc accagtgatc
    19441 tgaacagccg gatcctcacc aaacaggttg ggaagcgcat acagcagacc gatgacaatc
    19501 accacgatca gcatgacgta cttccacaaa ggataacggt ttaacacggc aattccctta
    19561 gggaaaaatt ttaattacag cgccttcatg gtgcctttcg gcaggacggc agctacgaag
    19621 tcacgtttaa taactacttc agtggtgtca ttcagcgcga tagcaatgta gccgttttcc
    19681 gctactttgg ttacgcgacc aaccaggcca ccgttcgtca gaacttcatc acctttggca
    19741 atggagtcca tcagcttttt gtgttctttg gtgcgcttct gctgtggacg caggatcatg
    19801 aaatagaaaa tcagaccgaa taccaccagc atcaaaatca aagacatcgg gctaccttgc
    19861 gccggtgcac ccgttgccgc taccgcatca gaaataaaaa agctcattaa atttccctca
    19921 ttattaatat taatcaacgt tcaaaggtgg tacttctcgc ccctgacgct ggtaaaaatc
    19981 agttacgaag ctctctaatt taccctcttc aatagccttg cgtaaacccg ccatcaaacg
    20041 ctggtagtaa cgaaggttat gaatggtgtt gagtcgcgcg cctaatattt cgttgcaacg
    20101 gtcgagatga tgcaagtaag cgcgtgaata attgcgacag gtgtagcaat cacactcagg
    20161 atcgagtggg ccagtatcgc tcttatattt cgcattgcgg attttcacca cgccatcggt
    20221 cacgaacaaa tgaccatttc gggcgttgcg ggttggcatt acgcagtcaa acatatcgat
    20281 accgcgacgt acgccttcaa ccaggtcttc cggtttacca acgcccatca ggtaacgcgg
    20341 tttgtctgcc ggaatttgcg gacacacatg ctccagaata cggtgcatat ctgctttcgg
    20401 ctcacccaca gccagaccgc cgacagcgta gccatcaaag ccgatatcta ccagaccttt
    20461 aacagatata tcacgtaaat cttcgtaaat actgccctga attataccaa ataacgcatt
    20521 tttgttacca agactgtcaa aacgctcacg gctacgcttc gcccaacgca gagacatctc
    20581 catagagcgt tttgcgtaat cccagtcagc aggatacggc gtacactcat caaagatcat
    20641 gacgatatcc gaaccaagat cgtactgaat ctccatcgac ttttccgggt cgaggaaaat
    20701 cgggtcgccg ttgatcgggt tacggaagtg aacgccctgt tcggtgattt tacgaatatc
    20761 accaaggctg aagacctgga agccgccgga gtcggtaaga atcggtcctt tccactgcat
    20821 aaaatcgtgc agatcgccgt gcagtttcat gatttcctgg cccgggcgca gccacaggtg
    20881 gaaagtgttg ccgaggataa tttgcgcgcc agtggcttca acttcttccg ccgtcatccc
    20941 ttttacggtg ccgtaggtgc caacaggcat aaaacaaggc gtttccacta cgccacgatc
    21001 aaagaccagg cggccacggc gtgcgcgacc gtcggtggtg tccagttcaa atttcatttt
    21061 ttctccacta cgtcagaaaa acagtccaac gtttaaacca gcgccgcgga attactcccc
    21121 gacgcgctca ttaattgcct gcggattgta cgtgataaac atcgcatcac cgtaactaaa
    21181 aaagcgatat ttctcttcta ccgccgcttt ataggcattc atggtgtgtt gataaccggc
    21241 aaaggccgaa accagcataa tcagcgtcga ctctggcaag tggaagttcg tcaccagcgc
    21301 atcgaccact ttgtactgga agcccggata gataaagatt tgggtatcgt caaagaacgg
    21361 ttcgataaga tcgttttttg ccgcctgtgc cgcgctttcc agcgaacgta ctgaagtggt
    21421 gccaaccgca atcacccggt taccgcgcgc tttcgccgcc agtaccgcgt ctaccacatc
    21481 ctgcggtact tcagcgtatt ccgagtgcat gatgtgatct tcaatggtgt cgacgcgcac
    21541 cggctggaag gtgcccgcac caacgtgcaa cgtcacaaac gccatctcca cgcctttggc
    21601 gcgtaatttt tccagcaaag gctcgtcaaa atgcagacca gcggtcgggg ctgcaaccgc
    21661 gcccggtttt tcgctataaa cggtttgata aagttcgcgg tcagcgtctt cgtccggacg
    21721 atcgatatac ggcggcagcg gcatatggcc gatgctgttg aggatatcca gtaccgagcg
    21781 ttcatcatta aattcgactt caaacagtgc gccgtggcgc gcggtcattg ttgcgttgat
    21841 actttcgtca tcgcccagca acagttctgc gccaggtttt ggcgctttcg aggcgcgaat
    21901 atgcgcaaga atgcgtttgt cgtcgagcat ccgctcaacc agcacttcaa tcttgccgcc
    21961 gctggcttta cgcccaaaca gacgcgccgg gatcacgcgg gtattattaa aaaccagaag
    22021 atcgccgggg ttgagcttat cgagtaaatc ggtgaaagta ccgtgcgtca gcgcacccgt
    22081 cggcccgtcc agcgacagta aacgacagct actgcgttca ggcatgggat agtgggcaat
    22141 cagggattcg ggcaattcaa aggagaaatc ggtaacgcgc atgacactga ctcagactaa
    22201 aataagagtc gggtagtcta gtgccggggc gctctccctg caacctttac ccccctttgg
    22261 atagaatata atcgatgaat tttttagctc acctgcattt agcccatctc gcggaaagct
    22321 cgctttctgg caatttactg gctgatttcg tacgcggaaa tcctgaagaa agttttccgc
    22381 ccgacgtcgt ggctggcatt catatgcatc gacgtattga ctgacaatct gccggaagtc
    22441 cgcgaagcac gggagtggtt tcgtagtgaa acgcgccgcg ttgcgcctat tacgctggat
    22501 gtcatgtggg atcactttct ttcccgccac tggtcgcagc tatcgccgga ctttccgcta
    22561 caggaatttg tctgttatgc ccgcgagcaa gtgatgacga ttttgccgga ctcaccgcca
    22621 cgttttatca atctgaacaa ttacttgtgg tcagagcagt ggctggtgcg ctatcgcgat
    22681 atggatttca tccagaacgt gttaaacggc atggcaagcc gccgcccacg tctggatgcc
    22741 ctgcgtgact cctggtacga tttagacgct cattatgacg ccctcgaaac ccgcttctgg
    22801 cagttttatc cgcggatgat ggcgcaggcg tcacacaagg cgttataaaa cgttagttca
    22861 tccataccgt agccgaaatg gcaggcaaag ccagaatccc gtcagtcagt tgcccatgcc
    22921 cttctttgca ttgccattgc acggcattga gaaacggtga cgcgggtagc accacttcac
    22981 tggcctcgcc acggttgatt gccaccagta cacgttgctg attcagcacg cggacaaata
    23041 ccactacgtt atcttccgca tacagcacct gacagccgcc acgacgcagc gcctgacttt
    23101 tcttacgcag cacaatcatt cgccggtaca gcgcgaataa cgccgtatcc tgcttttcca
    23161 cctgccaggg gaacggttta cggcaagacg gatcgttttt accatccagt cccacttcat
    23221 caccgtaata aatgcacggt acaccaggcc aggtgaacag ccagaccacc gccagcggca
    23281 ggcgcgcaat atcccgaccg agcagcgttt taaatcgcgc agtatcgtgg ctatcgagct
    23341 gattaaacat tcgtaattgt tgttgatgag aaagccctgc gcggtaatta tccatccagg
    23401 ccatacaggt ttgggcgtca atttgctgcg gatcataaga gatgtcggta ttggcaagaa
    23461 atccccacaa cgggaatgtg aagccacgat agttcatggc agcatcttcc acatcggcct
    23521 gtaaccattg ccgtgcatcg ccaaaatgtt cgccgacaat atacgcttcc gactgggttt
    23581 ctttcgccgc ttcggtgatc ccggcaacgt gctgcatatt attgcgcgcc ccacccgcct
    23641 cccccagcat atgcaccaca tccagccgcc agccgtccat attccacggc gctttcagcc
    23701 agtggcggac aatactgtct tccccgcgat aaatttcatt caccagactt tccgactgat
    23761 aatccagctt cggcaagctg gcatagccaa gccagtcgag cgccgtgcca tcatcactaa
    23821 acaagtacca gtcgcgccag ggcgattcag agttgtgaca agctcccccc gtgccaagat
    23881 tgtgcctgtc aaaccaggca tgggaatcgc cactgtggtt aaacacgccg tccagcacca
    23941 gccgcattcc cagctgctgc gtattgtgtc gcaaacgcag caacgcccca tcaccgccaa
    24001 actgcggatc gacatggcga taatcctcgg tatcgtattt atgtacgctg ggagctttaa
    24061 acaccggatt gagatacagc gctgtcacgc caagcttttt cagatacggc agtttttcgc
    24121 ttatcccgtc cagatcgccg ccatagaacg ttgatccgcc cgcctgcgcc gtgaccggtt
    24181 catcccagtc acgcaagatg atctcttgtc cggctgcatg atggtaatag acatgatcct
    24241 gttcagcttc acgaggaaga ctacgcgcaa aacgatcagg gaagatctga taaaaaatct
    24301 gatccgcagc ccattgtggg ccgatatccg gtacatcgac ggcaaactgc tccagtcgtg
    24361 ccggcggcat tcggctgaag ccctgcggtg taaaccaacg ctggcgatcg tgccacagca
    24421 gtttgaaact gtaacgccgc cggggttgtc cgctggagag atcaatcgcc gcacgccatg
    24481 cggtgacgcc tggctgcggc tgactgcgct gcttatgcat tggtactgac atttcttcgt
    24541 tatcgtgttc tgtacgcagc ataatgcgct gcggtgggtc ttcgcccgtc agccacagtg
    24601 taatgagcag ttgatctttg ctttgtttaa caaatggggg caccggcagg tgccatgcat
    24661 ttaacatcat aattcccctg tgatgaaaat ctgatcacct tgccatagcc taatgcagga
    24721 tcactcatca tctgaaggat atttggggga ggaggacggg ggcggataac tttcggaata
    24781 gccggatcag gatttcctcc tgaaccggct tacagaacgc aagtattatt ggttttcagc
    24841 cagctgacga tcgtggcggc gtttaaacat ccagccaatc aacagcacaa caatccacgc
    24901 gaaaccgaca tacagcgaga tacgcgtatc cgggtgataa ccaatcaacc cgataataaa
    24961 gagcaggaaa atcagcccgc cgatggtcgt tgctacccca cccggcactt taaatttcag
    25021 cgccttaact tcttctggcg gcaaacggcg acggaaggcg atttgcgaca gcaggatcat
    25081 aatccacacc cataccgtgg cgaaggttgc cagcgaagcg atcaccagga agacgttttc
    25141 cggcatgatg tagttcagat acaccgcaaa cagcagcgcg gtagtcatca ccagcaccgt
    25201 aacccacgga ataccgcgac gcgacgtttt gctgaaaatt ttcggtgcgc tgccctgctc
    25261 tgccataccg tggagcatac ggcctacgcc aaatacgtca ctgttaattg ccgacagcga
    25321 agcggtcagc acaacaaagt taagaatgct ggcggcaaag gtaatgccca tatgctggaa
    25381 cgtcagcacg aacgggctac cggcagtgcc aacctgattc cacgggtaga tagacataat
    25441 gacgaacagc gtaccgacgt agaacaccag aatacgcatc ggcacggagt taatcgcacg
    25501 cggaatcgat ttctcaggat ctttcgcttc accggcggta atcccgataa tttcgatccc
    25561 accgtaagca aacatcacca tttgcaacga cattaccatg ccaagccagc cgttactgaa
    25621 gaagccgccg ttgctccaca ggttatgaat accggtcggt tgcccgccgt tgccaatccc
    25681 ccagatgatg atgccgaaac cggcgacaat catgatgatg atggtggcga ctttaaagaa
    25741 cgagaaccag aattccagct caccgaagac cttcacgctc atcaggttta cggcgcaaat
    25801 gatcagcacc acgctcagta cccaaatcca gtgcggcacc gtcgggaaca agacacccat
    25861 atagatacca aaagcggtca catcggcgat ggcgacaata aggatttcaa agcagtaggt
    25921 ccatccggta atgtaacctg ccagcgggcc aaggttttcc tgcgcataac gcgagaaaga
    25981 gctggcggcc gggttatgta ccgacatttc ccccagcgca cgcataatga tatacgccgc
    26041 gataccaccg ataatatagg ccaacaacac gctcggaccg gccattttga tggcgtctgc
    26101 cgaaccgtaa aacagcccgg tgccaattgc tgaacccagt gccataaagc gtatgtggcg
    26161 ggtacttagc ccacgcttta gcttattctt actttccatc aattcctaac ccatcaacac
    26221 aataaaaaac cacggggcat cgcagccccg tggttaaaac aaatgttcag tgatttagtg
    26281 agcgctggag gtcacctgac gacctgccgc gcgatcccag ataatggcca gaaccaccat
    26341 caccactgtt ggcattaacc acgccagacc ttgttcggcc agcggtaaac gctgcgccca
    26401 ggacggtaag atatcgctga atgcagatgc tttgatcccg tcgagaatac caaaaagcag
    26461 gctgataaac atcggcggag caatcacgcg ggacgaatta tgccaccatg agcgtgtaaa
    26521 acttaataca accagtgcga tacacggcgg ataaatagcg gtcagcaccg gtacggagat
    26581 ctggatcagc tggcttaagc cgaggttaga caccaccatc gagaagccgc cgaggataaa
    26641 caccagcgta cgataagaga gcggtacgta ctgggcaaag aattctgcac aagcacaggt
    26701 caggccaacc gccgtgacca gacaggcgat gaagattaac gccgccagca ggaagctacc
    26761 gccgccgcca aaggtgtgct gaacgtaagc atgcagaata gcagcgccgt ttgcagactg
    26821 atcgaccagc gacgcgctgt ctgaacccag acagaacagc gccaggtaca gcaaagtcag
    26881 accaacaccc gccatcaggc cagcccagac ggtataacgg gtcagcagac gcgcttcggt
    26941 aacgccacga gaacgcgccg cgttaacaat aacgatacca aacaccattg cgcccagcgt
    27001 atccatggtc agatagccgt taacgaagcc gttagaaaat gcagcgtttt gataagcctc
    27061 agtcgccgtg ctgatagaac ccgccggcca gacaatcgcg acaacagaca ggatgaccag
    27121 cgcgataatt ttcagcggcg caaggaagtt gcccacggta tccagcagct tgcccggata
    27181 gagcgaaacc agaataacga tagcgaaata gaccaggctg tagataaaca gcggcagcgc
    27241 ggaatcaccc gtcagcggcg caatccccac ttcaaaggaa acggtagctg tacgcggcgt
    27301 agcgaaaagc ggccccaccg ccaggtaaca aaccgttgcc agcagtacgc cagcgacttt
    27361 accgattggc gtgctgaggc tgtcaacacc gccgccaact tttgccagcg ccactaccgt
    27421 taataccggc aggccaacgg cagtaatgag gaagccgaat gccgcagtcc agacgtgttc
    27481 gcctgcctgt aagccgacca ttggagggaa aataatgtta cctgcgccga cgaacaacgc
    27541 aaatgtcata aagcccagag cgatgatatc gcgcgatctt aattgatggg tcataaatca
    27601 tactgcctgt ggatgtggtg ttgtaacgtt aaatttttgt gccaatccct ggggatgata
    27661 cagcggtata tttaaacaat tttagcggga aatactccca accaggcgcg gcgaagaata
    27721 gtttttcgat ttaccgcgtt aatgcagtca gtatgacagc acctgaagcg caatttaaac
    27781 gcttataacg ttttaaagca aggagggatc taaaaaccag aggtatatag cgataagaaa
    27841 tcaccaacca gtataaaaca ctatttccgt gaaaaagaca tggctaatca tgcgaacaaa
    27901 aaatcaccaa ccgttgtaaa atctaacgcc cgtattttga ctgcaaagaa aataagccag
    27961 catcggctgg cttatggcaa taaaagatga caaaggcgga ttaatcgctg tttttggcaa
    28021 ttaaacgttc cgggataaca aaactgaaac gtgttccttt tcctactgta ctctcaatat
    28081 tcaggcgact ttcgtgatga ttcaccgcat gtttcacgat cgctaaccct aatccgctac
    28141 cgccggtttg ccgggaacgc gctttatcaa cgcgataaaa acgctcggtc aggcgcggaa
    28201 tatgctccgg tgcaatgccc ggtccgttat cttcaacgct aaattcggca ccgtgcggca
    28261 ctcgctgcca gcgtacggtg atatgcgtgc cttccggcgt atgattcacg gcgttataga
    28321 ccaggttcga aatcgcactg cgtagctgat cttcgttgcc agacaccttg aggccgttat
    28381 ctatctcaaa ggtaaatgtc tgttttttct gactcagagt ctgagcctcg cgctcaacaa
    28441 cgcgcagcat catcggcaca tcaacctttt cattgagcaa ttgcgtcggc gaggcttcaa
    28501 ttttcgacag cgttagcaac tgcttcacca gcccttccat ccgctgggtc tgctcgcgca
    28561 tggtgtgcaa cgctttttcg cgtaccgcgc cttccagcgg ctgctcatcc atcatctcca
    28621 ggtaaccctg taacacggtc aatggcgtac gtaactcatg gctcacgttg gcaaaaaagt
    28681 tacgccgcgc cccttccagt tgatgcattt gcgtgacatc acgcgccacc atcaacaact
    28741 gtttgtgggt ataaggcatg acgcgaattt ccagatgccg cccggtgttg aacaccagat
    28801 tgagcgggcg agaaaaatca cgcgttttca gatattgcgt aaactccggg taacgcagta
    28861 ggttaaggat gttctgcccg ttatcttccg gccagcgcaa accaagaatt tgttgcgcca
    28921 gaccgttaca ccagaaaata ccgccctctt ccgtggtcag caccaccgcg tcgggcagcg
    28981 actccgcgcc gctacgaaag cgtttaatca gattgcccag ttcacggcgg cgttttttat
    29041 ttcgcagctg catctggtgt aagccgtata gtagcggttc ccagctacca cgccccggtg
    29101 gcggggtcat actgcgatcc acccacagcc accatgaaag gcgcaataaa ttccagaaat
    29161 gccagataag cagtcctgtt accgatgcca gcaaaaacca gggcaggtaa ccaaaaaatg
    29221 cacccaggat gaaagccggg aggcagcaaa gtagcagctc cagcaccagc cttttccacg
    29281 acagccgttc cagcacgtaa gatactccag ttaagaaatc ataagccctg ctctgcgtcc
    29341 gatgagcaag gcgttaaaag cgggttgaaa aacgatatcc tgtaccgcgc acggtctgca
    29401 ccatgcggtc atgcccgccg ggctccagtg ctttacgcag gcgacgaatg tggacatcga
    29461 ccgtgcggtc ttccacatac acgttagttc cccagacgtg gtttaacagc tgctcgcggc
    29521 tgtacacgcg ctcaggatgc gtcataaaaa agtgcagcag tttaaattct gtcggcccca
    29581 tctccagcgg ctcttcgccc gccatcactc ggtgagatgt cgggtcgaga cttaatccct
    29641 gcatctcaat cacctcttcc accgccattg gcgaaatacg gcgcattacc gctttgattc
    29701 gcgccaccag ctccttcggc gaaaatggct tggtgatata gtcatccgcg ccggtttcaa
    29761 ggccgcgcac gcgatcttct tcttcccctc tggcggtcaa catcaccact ggaatatccc
    29821 gggtcatcga ctcgcgcttg aggtgtttga tgaactggat accggagccg ccaagtaaca
    29881 tccagtcaag gagaattaaa tccggccaag gttcattcag ttgattcaca gcactgtcat
    29941 aatcttccgc ttcgaccggc tgaaagccat tttgttcgag cacgaagcag accatttcgc
    30001 gaattggagc ttcatcttct acgaccagaa tacgtctcgc catgatttgc cctgttgtaa
    30061 taaataggtt gcgatcatta atgcgacgtc attatgcgtc agatttatga cagatttatg
    30121 aaaagctcgt cgcacatatc ttcaggttat tgatttccgt ggcgcagaaa aaagcaaatg
    30181 gcacatctgt ttgggtataa tcgcgaccat gctttttcgc cagggaaccg ttatgcgcat
    30241 ccttcacacc tcagactggc atctcggcca gaacttctac agtaaaagcc gcgaagctga
    30301 acatcaggct tttcttgact ggctgctgga gacagcacaa acccatcagg tggatgcgat
    30361 tattgttgct ggtgatgttt tcgataccgg ctcgccgccc agttacgccc gcacgttata
    30421 caaccgtttt gttgtcaatt tacagcaaac tggctgtcat ctggtggtac tggcaggaaa
    30481 ccatgactcg gtcgccacgc tgaatgaatc gcgcgatatc atggcgttcc tcaatacaac
    30541 cgtggtcgcc agcgccggac atgcgccgca aatcttgcct cgtcgcgacg ggacgccagg
    30601 cgcagtgctg tgccccattc cgtttttacg cccgcgtgac attatttcca gccaggcggg
    30661 gcttaacggt attgaaaaac agcagcattt actggcagcg attaccgatt attaccaaca
    30721 acactatgcc gatgcctgca aactgcgcgg cgatcagcct ctgcccatca tcgccacggg
    30781 acatttaacg accgtgggtg ccagtaaaag tgacgccgtg cgtgacattt atattggcac
    30841 gctggacgcg tttccggcac aaaactttcc accagccgac tacatcgcgc tcgggcatat
    30901 tcaccgcgca cagattattg gcggcatgga acatgttcgc tattgcggtt cccccattcc
    30961 actgagtttt gatgaatgcg gtaagagtaa atatgtccat ctggtgacat tttcaaacgg
    31021 caaattagag agcgtggaaa acctgaacgt accggtaact caacccatgg cggtgctgaa
    31081 aggcgatctg gcgtcgatta ccgcacagct ggaacagtgg cgcgatgtat cgcaggagcc
    31141 acctgtctgg ctggatatcg aaatcactac tgatgagtat ctgcatgata ttcagcgcaa
    31201 aatccaggca ttaaccgaat cattgcctgt cgaagtattg ctggtacgtc ggagtcgtga
    31261 acagcgcgag cgtgtgttag ccagccaaca gcgtgaaacc ctcagcgaac tcagcgtcga
    31321 agaggtgttc aatcgccgtc tggcactgga agaactggat gaatcgcagc agcaacgtct
    31381 gcagcatctt ttcaccacga cgttgcatac cctcgccgga gaacacgaag catgaaaatt
    31441 ctcagcctgc gcctgaaaaa cctgaactca ttaaaaggcg aatggaagat tgatttcacc
    31501 cgcgagccgt tcgccagcaa cgggctgttt gccattaccg gcccaaccgg tgcggggaaa
    31561 accaccctgc tggacgccat ttgtctggcg ctgtatcacg aaactccgcg tctctctaac
    31621 gtttcacaat cgcaaaatga tctcatgacc cgcgataccg ctgaatgtct ggcggaggtg
    31681 gagtttgaag tgaaaggtga agcgtatcgc gcgttctgga gccagaatcg ggcgcgtaac
    31741 cagccggacg gtaatttgca ggtgcctcgc gtagagctgg cgcgctgcgc cgacggcaaa
    31801 attctcgccg acaaagtgaa agataagctg gaactgacag cgacgttaac cgggctggat
    31861 tacgggcgct tcacccgttc gatgctgctt tcgcaggggc aatttgctgc cttcctgaat
    31921 gccaaaccca aagaacgcgc ggaattgctc gaggagttaa ccggcactga aatctacggg
    31981 caaatctcgg cgatggtttt tgagcagcac aaatcggccc gcactgagct ggagaagctg
    32041 caagcgcagg ccagcggcgt cacgttgctc acgccggaac aagtgcaatc gctgacagcg
    32101 agtttgcagg tacttactga cgaagaaaaa cagttaatta ccgcgcagca gcaagaacaa
    32161 caatcgctaa actggttaac gcgtcaggac gaattgcagc aagaagccag ccgccgtcag
    32221 caggccttgc aacaggcgtt agccgaagaa gaaaaagcgc aacctcaact ggcggcgctt
    32281 agtctggcac aaccggcacg aaatcttcgt ccacactggg aacgcatcgc agaacacagc
    32341 gcggcgctgg cgcatattcg ccagcagatt gaagaagtaa atactcgctt acagagcaca
    32401 atggcgcttc gcgcgagcat tcgccaccac gcggcgaagc agtcagcaga attacagcag
    32461 cagcaacaaa gcctgaatac ctggttacag gaacacgacc gcttccgtca gtggaacaac
    32521 gaactggcgg gttggcgtgc gcagttctcc caacaaacca gcgatcgcga gcatctgcgg
    32581 caatggcagc aacagttaac ccatgctgag caaaaactta atgcgcttgc ggcgatcacg
    32641 ttgacgttaa ccgccgatga agttgctacc gccctggcgc aacatgccga gcaacgccca
    32701 ctgcgtcagc gcctggtcgc gctgcatggg cagattgttc cccaacaaaa acgtctggcg
    32761 cagttaatgg tcactatcca gaatgtcact ctggaacaga cgcaacgtaa tgtcgcactt
    32821 aacgaaatgc gccagcgtta taaagaaaag acgcagcaac ttgccgatat gaaaaccatt
    32881 tgcgagcagg aagcgcgcat caaaacgctg gaagcccagc gtgcacagtt acaggcgggt
    32941 cagccttgtc cactttgtgg ttccaccagc cacccggcgg tcgaggcgta tcaggcgctg
    33001 gagcctggtg ttaatcagtc tcgattactg gcgctggaaa acgaagttaa aaagctcggc
    33061 gaagaaggtg cggcgctgcg cgggcaactg gatgcattaa cgaagcagct tcagcgcgat
    33121 gaaaacgaag cgcaaagcct ccgacaagat gagcaagcac ttactcaaca atggcaagcc
    33181 gtcacggcca gcctcaatat caccttgcag ccacaggacg atattcaacc gtggctggat
    33241 gcacaagatg agcacgaacg ccagctgcgg ttactcagcc aacgacatga attacaaggg
    33301 cagattgccg cgcataatca gcaaattatc cagtatcaac agcaaattga acaacgtcag
    33361 cagcagctct taacggcatt ggcgggttat gcactgacat tgccacagga agatgaagaa
    33421 gagagctggt tggcgacacg tcagcaagaa gcgcagagct ggcagcaacg ccagaacgaa
    33481 ttaaccgcgc tgcaaaaccg tattcagcag ctgacgccga ttctggaaac gttgccgcaa
    33541 agtgatgatc tcccgcacag cgaagaaact gtggagctgg ataactggcg gcaggttcat
    33601 gaacaatgtc tcgcattaca cagccagcag cagacgttac agcaacagga tgttctggcg
    33661 gcgcaaagtc tgcaaaaagc ccaagcgcag tttgacacca cgctacaggc cagcgtcttt
    33721 gacgatcagc aggcgttcct tgcggcgcta atggatgaac aaacactaac gcagctggaa
    33781 cagctcaagc agaatctgga aaaccagcgc cgtcaggcgc aaacgctggt cactcagaca
    33841 gcagaaacgc tggcacagca tcaacaacac cgacctgacg ggttggctct cactgtgacg
    33901 gtggagcaga ttcagcaaga gttagcgcaa actcaccaaa agttgcgtga aaacaccacg
    33961 agtcaaggcg agattcgcca gcagctgaag caggatgcag ataaccgtca gcaacaacaa
    34021 accttaatgc agcaaattgc tcaaatgacg cagcaggttg aggactgggg atatctgaat
    34081 tcgctaatag gttccaaaga gggcgataaa ttccgcaagt ttgcccaggg gctgacgctg
    34141 gataatttag tccatctcgc taatcagcaa cttacccggc tgcacgggcg ctatctgtta
    34201 cagcgcaaag ccagcgaggc gctggaagtc gaggttgttg atacctggca ggcagatgcg
    34261 gtacgcgata cccgtaccct ttccggcggc gaaagtttcc tcgttagtct ggcgctggcg
    34321 ctggcgcttt cggatctggt cagccataaa acacgtattg actcgctgtt ccttgatgaa
    34381 ggttttggca cgctggatag cgaaacgctg gataccgccc ttgatgcgct ggatgccctg
    34441 aacgccagtg gcaaaaccat cggtgtgatt agccacgtag aagcgatgaa agagcgtatt
    34501 ccggtgcaga tcaaagtgaa aaagatcaac ggcctgggct acagcaaact ggaaagtacg
    34561 tttgcagtga aataactatt cagcaggata atgaatacag aggggcgaat tatctcttgg
    34621 ccttgctggt cgttatcctg caagctatca ctttattggc tacggtgatt ggtagccgtt
    34681 ctggtggttg tgatggtggt atgaaaaaag tcattttatc tttggctctg ggcacgtttg
    34741 gtttggggat agccgaattt ggcattatga gcgtgctcac ggagctggcg cataacgtag
    34801 gaatttcgat tcctgctgcc gggcatatga tctcgtatta tgcactggtg gtggtggtcg
    34861 gtgcgccaat catcgcactc ttttccagcc gctacttact caaacatatc ttgttgtttc
    34921 tggtggcgtt gtgcgtcatt ggcaacgcca tgttcacgct ctcttcgtct tacctgatgc
    34981 tcgccattgg tcggctggta tccggctttc cgcatggcgc attttttggc gtcggagcga
    35041 tcgtgttatc aaaaattatc aaacccggaa aagtcaccgc cgccgtggcg gggatggttt
    35101 ccgggatgac agtcgccaat ttgctgggca ttccgctggg aacgtattta agtcaggaat
    35161 gctggcgtta caccttttta ttgatcgctg tttttaatat tgcggtgatg gcatcggtct
    35221 atttttgggt gccagatatt cgcgacgagg cgaaaggaaa tctgcgcgaa caatttcact
    35281 ttttgcgcag cccggccccg tggttaattt tcgccgccac gatgtttggc aacgcaggtg
    35341 tgtttgcctg gttcagctac gtaaagccat acatgatgtt tatttccggt ttttcggaaa
    35401 cggcgatgac ctttattatg atgttagttg ggctagggat ggtgctggga aatatgctaa
    35461 gtggcaggat ttcaggacgt tattcaccac tgcgcattgc agcagtgact gactttatca
    35521 ttgtactggc actgctgatg ctctttttct gcggcggcat gaaaacaacg tcgcttattt
    35581 ttgcttttat ttgttgcgcg ggattatttg ccctttcagc acctctgcaa atattgttac
    35641 tgcaaaatgc caaaggcgga gagttattag gtgccgcagg tgggcaaata gcgtttaacc
    35701 tcggtagcgc cgtcggcgca tattgcggag gtatgatgct gacgctgggg ctggcatata
    35761 attacgtggc gctgcctgcc gccctgtttt cgtttgctgc gatgtcatcg ttgctgctgt
    35821 atggtcgcta taagcgccag caagcggcgg atagtccggt gctggcgaaa ccactggggt
    35881 agattactgt cacggtgcgc gttgcttatt gccggatgcg gcgtgaacgc cttatccgcc
    35941 ctacgcggtt ctggcacatt ttgtaggcct gataagacgc ggcaagcgtc gcatcaggca
    36001 tgatgcgcca attgcctacg ttttttactc ttgtggccat aaccacgcag cgccgcgtac
    36061 gccgctggaa tcaccgtgct tcgccttact caccggcgtt tcacattcgc cgccgaagac
    36121 aaattgttta atcaactgcc caaccgtttg atataaacgg tctacattgc tcatcccgcc
    36181 ccccaggaca atcacatccg gatcgagaat attcacgaca tgtgccagcg attttgccag
    36241 ccgcagctcg tagcgacgca atgccagttc cgctaccgga tcgctttctt caaccaggcg
    36301 gataatttca ctgcctttca gcgcatgtcc gctcaaacga cgataatccg tcgcgaatcc
    36361 cgtgcccgaa ataaaggttt caatacaacc ttgtttaccg caataacaag ggacttcctc
    36421 gcgataacgc agttcgtctt cgtccatcca cggtagcgga ttgtgtcccc actcacctgc
    36481 cgtgccattg ccgccgatat gcgtccgccc attgaatgcc acgcccgcgc cgcatcccgt
    36541 gccgataatc acggcaaata ccgtctgcgc tcccgctgcc gcgccatcta ctgcttctga
    36601 aaccgccaga cagttggcat catttgccag ccgcacttcc cgctgcaacc tcgcgcttaa
    36661 gtctttatcg aatggctgac cgttgagcca ggttgaattg gcattcttca ccacaccggt
    36721 gtagggcgaa atcgaacctg gaatgcccat acctaccgtt ccgcgctgcc ctgtcgcctg
    36781 ctccgccata tcaaccaacg tggcgatcgt ttcaatagtc tgccggtaat catcacgcgg
    36841 cgtgggcaga cgatggcggt acaactgctc ccctgcatcg cccagtgcaa tcacttcagt
    36901 tttggtgccg cctaaatcga tacctatacg cactgtactc tccttatttt tttcaatatc
    36961 aatagcgtag agacggacaa ccggattggc aatgcaaggc cgcctacaat tcgttatcat
    37021 gcccgctaaa tttaacgaca aggccgtgga aattatcatg ctgtggttca aaaatttaat
    37081 ggtttaccgt cttagccgcg agatttcgct gcgtgcagaa gagatggaaa aacagctagc
    37141 ctcgatggca tttaccccat gcagcagcca ggacatggcg aagatgggct gggttcctcc
    37201 gatgggatcg cacagcgatg cgttaacgca cgttgccaat ggtcaaattg ttatctgcgc
    37261 gcgcaaagaa gaaaaaatcc tcccgtctcc ggtgattaaa caggcgctgg aagcgaaaat
    37321 cgccaaactg gaagcggaac aggcgcgtaa gctgaagaaa accgaaaaag attcgctgaa
    37381 agacgaagtg ctgcactctc tgctgccgcg tgctttcagc cgttttagcc agacaatgat
    37441 gtggctcgac acggttaacg gtttgattat ggtggactgc gccagtgcca aaaaagcgga
    37501 agatacgctg gcattactgc gtaaaagcct ggggtcgtta ccggttgtac cattgagcat
    37561 ggaaaacccg attgaactga cgctgaccga atgggttcgc tccggtagtg cggcacaggg
    37621 cttccagctg cttgatgaag ccgagctgaa atcgttgctg gaagatggcg gcgtgatccg
    37681 cgcgaagaaa caagatctga ccagcgaaga gatcaccaat cacattgaag ccggaaaagt
    37741 ggtgactaaa ctggcgctcg actggcagca gcgcattcag tttgtgatgt gcgacgatgg
    37801 ttcgctcaag cgtcttaagt tctgcgacga gctgcacgat caaaacgaag atatcgaccg
    37861 tgaagatttc gcccagcgtt ttgatgccga tttcatcctg atgactggtg aactggcagc
    37921 gttaattcaa aacctgattg aaggattagg tggcgaagca caacgttaat tgctgatttt
    37981 cctttaatgc cggatgcgac gcctgccgcg tcttatccgg cgtacgaagc cacaccaggc
    38041 atataattat tcgctacggc gagcaataat ttttagcgca gcaatattat gcgttttacg
    38101 ccgtaactga tctaatcgcc tcatccctat tcgcctgcca gcagcacgca gaatattcac
    38161 taacccccat ttatcgctgg taaattgttt tgttaacagc tccgcttttg gggtatcaat
    38221 atattcacgc aataaatcag aaattgaaca aatatccgtc aggtaaggat accagcaagt
    38281 ttcaccgtga aaattacgca ctccagcgtt acctgcaata aagtctttcg ggtaatccca
    38341 gatcacgttt ttatccaacg tgatccaata acgcggtaaa tcggtacttc caccattttg
    38401 gctgcgcatg ggataacggg tacagtgaat ttgcaggttg atcgttggcg tcagtaagtc
    38461 atagatctcc cgctgtaact tgctccatgg acgttgtgtc attgtttttc ctcaagccgg
    38521 tgacgcgctt cactgcgaat tttccgcgtt gccccggttt tcactaaatc acgtaactgc
    38581 gaaagatcgg gttgttcgac aaataagagt aatgatggat cctcttttaa ccacgccgtt
    38641 ttcacatcgg cagccagctg tggattatta atagccagcg accgatcctg aggctcagtc
    38701 aatgtcgtta acaacgacgc cggggtatgt gcgttgcgta atgccgtttg gcgcaaatga
    38761 tcattgcgac ttt
//
LOCUS       DAJTYG010000027        38743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTYG010000027 DAJTYG010000000
VERSION     DAJTYG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.027"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="OO568_001519"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="OO568_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR6640920.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..580
                     /gene="hisL"
                     /locus_tag="OO568_001520"
     CDS             530..580
                     /gene="hisL"
                     /locus_tag="OO568_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6640921.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    556..679
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            726..1625
                     /gene="hisG"
                     /locus_tag="OO568_001521"
     CDS             726..1625
                     /gene="hisG"
                     /locus_tag="OO568_001521"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6640922.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1631..2935
                     /gene="hisD"
                     /locus_tag="OO568_001522"
     CDS             1631..2935
                     /gene="hisD"
                     /locus_tag="OO568_001522"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6640923.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2932..4002
                     /gene="hisC"
                     /locus_tag="OO568_001523"
     CDS             2932..4002
                     /gene="hisC"
                     /locus_tag="OO568_001523"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6640924.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            4002..5069
                     /gene="hisB"
                     /locus_tag="OO568_001524"
     CDS             4002..5069
                     /gene="hisB"
                     /locus_tag="OO568_001524"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6640925.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KEGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5069..5659
                     /gene="hisH"
                     /locus_tag="OO568_001525"
     CDS             5069..5659
                     /gene="hisH"
                     /locus_tag="OO568_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6640926.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5659..6396
                     /gene="hisA"
                     /locus_tag="OO568_001526"
     CDS             5659..6396
                     /gene="hisA"
                     /locus_tag="OO568_001526"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6640927.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6378..7154
                     /gene="hisF"
                     /locus_tag="OO568_001527"
     CDS             6378..7154
                     /gene="hisF"
                     /locus_tag="OO568_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6640928.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7148..7759
                     /locus_tag="OO568_001528"
     CDS             7148..7759
                     /locus_tag="OO568_001528"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6640929.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="OO568_001529"
     CDS             complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="OO568_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6640930.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="OO568_001530"
     CDS             complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="OO568_001530"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR6640931.1"
                     /translation="MKITISGTSYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRTSSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="OO568_001531"
     CDS             complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="OO568_001531"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6640932.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11999..12358)
                     /locus_tag="OO568_001532"
     CDS             complement(11999..12358)
                     /locus_tag="OO568_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640933.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12355..12861)
                     /locus_tag="OO568_001533"
     CDS             complement(12355..12861)
                     /locus_tag="OO568_001533"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640934.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLLVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFIMNT"
     gene            complement(13007..13570)
                     /locus_tag="OO568_001534"
     CDS             complement(13007..13570)
                     /locus_tag="OO568_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640935.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPSIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13778..14716)
                     /locus_tag="OO568_001535"
                     /pseudo
     CDS             complement(13778..14716)
                     /locus_tag="OO568_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14779..15153)
                     /locus_tag="OO568_001536"
     CDS             complement(14779..15153)
                     /locus_tag="OO568_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR6640936.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15157..16305)
                     /gene="wzy"
                     /locus_tag="OO568_001537"
     CDS             complement(15157..16305)
                     /gene="wzy"
                     /locus_tag="OO568_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR6640937.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16325..17230)
                     /gene="rfbG"
                     /locus_tag="OO568_001538"
     CDS             complement(16325..17230)
                     /gene="rfbG"
                     /locus_tag="OO568_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR6640938.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17223..18113)
                     /locus_tag="OO568_001539"
     CDS             complement(17223..18113)
                     /locus_tag="OO568_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR6640939.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18143..19399)
                     /gene="wzx"
                     /locus_tag="OO568_001540"
     CDS             complement(18143..19399)
                     /gene="wzx"
                     /locus_tag="OO568_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR6640940.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19415..19960)
                     /gene="rfbC"
                     /locus_tag="OO568_001541"
     CDS             complement(19415..19960)
                     /gene="rfbC"
                     /locus_tag="OO568_001541"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR6640941.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19965..20843)
                     /gene="rfbA"
                     /locus_tag="OO568_001542"
     CDS             complement(19965..20843)
                     /gene="rfbA"
                     /locus_tag="OO568_001542"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6640942.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20902..21801)
                     /gene="rfbD"
                     /locus_tag="OO568_001543"
     CDS             complement(20902..21801)
                     /gene="rfbD"
                     /locus_tag="OO568_001543"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR6640943.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21801..22886)
                     /gene="rfbB"
                     /locus_tag="OO568_001544"
     CDS             complement(21801..22886)
                     /gene="rfbB"
                     /locus_tag="OO568_001544"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6640944.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23259..24152)
                     /gene="galF"
                     /locus_tag="OO568_001545"
     CDS             complement(23259..24152)
                     /gene="galF"
                     /locus_tag="OO568_001545"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR6640945.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24327..25721)
                     /gene="wcaM"
                     /locus_tag="OO568_001546"
     CDS             complement(24327..25721)
                     /gene="wcaM"
                     /locus_tag="OO568_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR6640946.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLNHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFIRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25732..26952)
                     /gene="wcaL"
                     /locus_tag="OO568_001547"
     CDS             complement(25732..26952)
                     /gene="wcaL"
                     /locus_tag="OO568_001547"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR6640947.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26949..28229)
                     /gene="wcaK"
                     /locus_tag="OO568_001548"
     CDS             complement(26949..28229)
                     /gene="wcaK"
                     /locus_tag="OO568_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR6640948.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28409..29887)
                     /gene="wzxC"
                     /locus_tag="OO568_001549"
     CDS             complement(28409..29887)
                     /gene="wzxC"
                     /locus_tag="OO568_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR6640949.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29889..31283)
                     /gene="wcaJ"
                     /locus_tag="OO568_001550"
     CDS             complement(29889..31283)
                     /gene="wcaJ"
                     /locus_tag="OO568_001550"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR6640950.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLAHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGGNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31338..32708)
                     /gene="cpsG"
                     /locus_tag="OO568_001551"
                     /gene_synonym="manB"
     CDS             complement(31338..32708)
                     /gene="cpsG"
                     /locus_tag="OO568_001551"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6640951.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32813..34249)
                     /gene="cpsB"
                     /locus_tag="OO568_001552"
     CDS             complement(32813..34249)
                     /gene="cpsB"
                     /locus_tag="OO568_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6640952.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADKDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34252..35464)
                     /gene="wcaI"
                     /locus_tag="OO568_001553"
                     /pseudo
     CDS             complement(34252..35464)
                     /gene="wcaI"
                     /locus_tag="OO568_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
     gene            complement(35461..35940)
                     /gene="gmm"
                     /locus_tag="OO568_001554"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35461..35940)
                     /gene="gmm"
                     /locus_tag="OO568_001554"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6640953.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35940..36908)
                     /gene="fcl"
                     /locus_tag="OO568_001555"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35940..36908)
                     /gene="fcl"
                     /locus_tag="OO568_001555"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6640954.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENSQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36911..38032)
                     /gene="gmd"
                     /locus_tag="OO568_001556"
     CDS             complement(36911..38032)
                     /gene="gmd"
                     /locus_tag="OO568_001556"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6640955.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38058..38606)
                     /gene="wcaF"
                     /locus_tag="OO568_001557"
     CDS             complement(38058..38606)
                     /gene="wcaF"
                     /locus_tag="OO568_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6640956.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38622..>38743)
                     /locus_tag="OO568_001558"
     CDS             complement(38622..>38743)
                     /locus_tag="OO568_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR6640957.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10345 a         9693 c         8718 g         9987 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgag ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcaccatca tcctgactag tctttcaggc gatgtgtgct
      601 ggaagacatt cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac
      661 cttccggggg cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata
      721 agaaaatgac agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg
      781 atgactcacg cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc
      841 tgatcgcgat ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc
      901 ccggtctggt aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag
      961 aagagctgct taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc
     1021 tggatttcgg cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc
     1081 cgctctcctt aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc
     1141 tcgaccagaa aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc
     1201 cgcgtgccgg gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag
     1261 ctaacggcct gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg
     1321 atggcgaaat ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg
     1381 gtgtgatcca ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctag
     1441 atgaagtcat cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg
     1501 atcaacagcg cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg
     1561 aaaaactgaa agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg
     1621 agtgatcgcc atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca
     1681 acgccagctg ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa
     1741 cgatattctc gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa
     1801 gtttgataaa accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag
     1861 cgaacgcctg agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac
     1921 cttccacact gcgcaaaaac tgccgccggt agatgtagaa acgcagccag gcgtgcgttg
     1981 ccagcaggtc acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc
     2041 gctcttctca acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt
     2101 ggtgctgtgc tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga
     2161 tgtgcaggac gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac
     2221 ggaatctgtg ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc
     2281 aaaacgccag gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc
     2341 ggaagtgctg gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct
     2401 ttctcaggct gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat
     2461 ggcgcgtcgc gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac
     2521 cacccgccag gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt
     2581 ggagatctcc aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga
     2641 actggtcgat ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc
     2701 ggcaggcgac tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac
     2761 ctgttccagc ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa
     2821 agaaggtttc tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac
     2881 cgcccacaaa aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc
     2941 gtgactatta ctgatttagc gcgtgaaaac gtccgcaacc tgacgccgta tcagtcggcg
     3001 cgtcgtctgg gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg
     3061 gagtttaagc ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg
     3121 attgaaaatt acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggt
     3181 gcggacgaag gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc
     3241 ctctactgcc cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa
     3301 tgccgcacag tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag
     3361 ctggacggcg taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc
     3421 aatccgcagg attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc
     3481 gatgaagctt atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat
     3541 ccgcacctgg cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc
     3601 ggatttacgc tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac
     3661 ccgctctcga cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc
     3721 gccatgcgcg aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg
     3781 aaagagatcc cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc
     3841 tttaaagcct ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat
     3901 cagaataaac aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa
     3961 agccagcgcg tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt
     4021 ttatcgatcg cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt
     4081 ttgacaaact cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagagg
     4141 gctacaagct ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag
     4201 cggattttga tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt
     4261 ttgatgaagt gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga
     4321 aagtaaaact ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg
     4381 taattggcga tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac
     4441 gctacgaccg cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc
     4501 gttacgccca tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg
     4561 atcgcgaagg tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc
     4621 agatcgccac ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg
     4681 acgatcacca caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc
     4741 tcggcgacaa acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg
     4801 cccgctgcgc actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct
     4861 accagcgtgt gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt
     4921 acaccatggg cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag
     4981 aaagcctgtt caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca
     5041 ccctgccctc gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc
     5101 caacctgaac tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg
     5161 tgacccggac attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca
     5221 agcagcgatg gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca
     5281 accggtgctg ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa
     5341 tggcgtcgac ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct
     5401 gccactgcca catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca
     5461 ggggattgaa gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc
     5521 gtggaccatc gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa
     5581 cttctacggc gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa
     5641 aaacttcctg gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg
     5701 cgtctccatc agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc
     5761 ttacaggatt acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca
     5821 aaagatccgg ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc
     5881 ccggtgcagg ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct
     5941 ggcgttgcgc gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc
     6001 tggtttgaac gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag
     6061 caaggcaaca agcaagtggc agtcagcggc tggcaagaga actcgggcat ttcactggaa
     6121 caactggtgg aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg
     6181 cgcgacggca cgctggcagg atctaacgtc tctttatatg aagaagtgtg tgccagatat
     6241 ccgcaagtgg cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta
     6301 cgtggcactg gcgtacgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc
     6361 gtgaaggagg ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg
     6421 gtcaggtggt gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac
     6481 tggcaaaacg ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt
     6541 ccagcgatgg ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca
     6601 ttccgttttg tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct
     6661 ttggcgcgga taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc
     6721 gcctggccga tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg
     6781 ccgaaaccgg taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca
     6841 ctcaatggga aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg
     6901 tcctcaacat gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa
     6961 aagtgcgtga agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac
     7021 acttcctgga agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc
     7081 acaaacaaat aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga
     7141 tcaggatatg ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct
     7201 gatgccggtg attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa
     7261 cccggaagcc ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa
     7321 acagcgactg tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc
     7381 cccggactgc gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca
     7441 caaaggcacc agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact
     7501 ggaacaactg ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact
     7561 gtatgccagc ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc
     7621 actggcagca acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta
     7681 tcacctgctg gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct
     7741 gcataaacgg catcagtgag ttacgtgctg agcaggtgtg atgttgttgt cacacccgga
     7801 gccattgccc gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg
     7861 ataagatgcg ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc
     7921 gtttctctta aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc
     7981 caacacaatc cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc
     8041 ctttttcgga ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg
     8101 aatatcaaga tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata
     8161 gtagtttggt gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc
     8221 ttcgctccct agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg
     8281 ctttgtcacc tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag
     8341 atctttctgc tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg
     8401 tcccagagca atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac
     8461 ttgctgaatg tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga
     8521 aacagtcaat ggtaattgtt ggttcttaac agaaggttcg atggtaagtt tttctggctc
     8581 ttcctgatta tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa
     8641 cgtctcctgc aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt
     8701 gttatagcca gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt
     8761 ctccttcgct actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat
     8821 aattgtcatc ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg
     8881 gtcatggttt tgcccagaaa cattattatt ttctactctc atcattaccc taactgacga
     8941 aaatttccaa gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata
     9001 aaaataatga attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa
     9061 caagatgtta gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa
     9121 gctcttctgc catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat
     9181 cacgttccag gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca
     9241 cttcaacgcc tttcgccttg atacgcttca taatcccctg aatagaagac gtacggaagt
     9301 tatctgaccc gctcttcata atcagacgat aaatacccac cacttgcggt ttgcgtgaca
     9361 aaatggcatc ggcaataaaa tctttacgcg tgcggtttgc atcgacaatt gccgagatca
     9421 ggttattcgg cacagactgg tagttcgcca gtaactgctt ggtatctttc ggcagacaat
     9481 aaccaccata accaaacgac ggattgttgt aatggttgcc aatacgcgga tcgagacaaa
     9541 cgccttcgat aatctggcga gtattcagac ctaaactttc tgcataacta tccagttcat
     9601 taaagtatgc tacgcgcatc gccagatagg tattagcgaa aagtttaatc gcttctgctt
     9661 cagtggagtc ggtaaacagg gtcgggatat tttgcttaat cgcgccttcc tgtaacaacg
     9721 cagcaaaacg ttctgcgcgt tctgaacgct caccgatgac aatacgtgac ggatggagat
     9781 tatcgtaaag ggctttacct tcgcggagga attccggaga gaaaataata ttttcagtac
     9841 gatatttctt atgcatcgct gcggtaaaac caacgggaac cgttgatttg atgaccataa
     9901 ccgcataagg atttatctca actacgtctt taattactga ttctacactg gatgtattga
     9961 aataattagt tttaggatca tagtcggttg gagtggcgat gatgacataa tcagcatccc
    10021 ggtaggcttc atttttatct aatgtggcat taaagtgtat tttatctgat tgcaaaaact
    10081 gctgaatttc cttatcaaca ataggagata tccgatcatt cagcatagca acgcgtgacg
    10141 gtaaaatatc taatgccaca acctcatgat tttgtgcgat tagaagcccg tttgacaagc
    10201 ctacatagct agtaccggaa atggtgattt tcatttcatg ctctcagaat taacttaact
    10261 gtgaatcatg atgtttttag catcgtgata agatgctaaa agttttaacg ctacgttgtc
    10321 agatggtcaa cgcaacgaaa aacaattcag attttcttta taagaatagc tgatgtttat
    10381 taagttaata ttaagcagtg aaatttagtc tataagatat ttggcaaaaa aaagcccggt
    10441 gcaatatacg ccgggcctca attttattgt tggttaaatc agattaatcc agccattcag
    10501 tatggaacac accttcttta tcaatgcgct tataagtatg cgccccgaaa tagtcacgct
    10561 gtgcctggat caggttcgca ggcagaaccg cggcacggta gctatcgtaa taggcaaccg
    10621 cagcggcgaa ggtcggaacc gggataccgt tctgtactgc ataagcgacg acatcgcgca
    10681 gcgcctgctg gtagtcatcg gcaatttgct tgaagtacgg ggccagcagc aggttagcga
    10741 tctgcggatt ttcggcataa gcatcggtga ttttctgcag gaactgcgca cggatgatgc
    10801 agccagcacg gaaaatcttc gcgatttcgc cgtagttcag atcccagttg tattcttcag
    10861 acgcagcacg cagctgagag aagccctgag cgtaagaaac gattttgccc agatacagcg
    10921 cactgcgaac tttttcgatg aactcagcct tgtcgcctgc tgactgagct tgcggaccag
    10981 agagaacttt agatgcggca acgcgctgat ctttcagaga agagatataa cgtgcaaaca
    11041 cagactcggt aatcagcgac agcggttcgc cgagatccag cgcgctctgg ctggtccatt
    11101 taccggtgcc tttgtttgct gcttcatcca gaatcacatc aaccaggtag ttaccgtctt
    11161 catctttttt ggtgaagata tctttagtga tgtcgatcag gtagctgcta agttcaccgt
    11221 tattccactc ggtgaaagtc tgggccagtt cttcattaga tagattcagg ccacctttga
    11281 gcagagaata cgcttcagca atgagctgca tatctccgta ttcaatacca ttgtgaacca
    11341 tcttcacata atggcctgcg ccgtcggcac caatataagt aacgcatggc tcgccgtctt
    11401 ctgccacggc agcgatttta gtcaggatag gcgcaaccag ctcataagcc tctttctgcc
    11461 cgccaggcat aatagaagga cctttcagcg cgccctcttc accgccggaa acaccggtac
    11521 cgatgaagtt aaagccctct gctgaaagct cacgattacg acgaatggtg tcctggaaga
    11581 aggtattacc accatcaatg atgatgtcac ctttatcgag gtatggtttc agggaatcaa
    11641 tcgcagcatc cgtgcctgca cctgctttca ccattaacag gatgcgacga ggcgtttcca
    11701 gagattcaac aaactctttc accgtatagt aaggagccag tttcttgcct ggattttcag
    11761 caatcacttc ttccgtcttt tcgcgggagc ggttgaaaat agagacggta taaccacgac
    11821 tttcgatgtt gagcgcaagg ttgcgcccca tcactgccat accgactacg ccgatctgtt
    11881 gctttgacat tgtttactcc tgtcttctat attattaacc gtctattaac cgttatatta
    11941 ttaaccgtcg tgattttcat cttctaatca aggaatacat atggttacag catgcttctt
    12001 actttatagc gataagtatg ccattgggtg tatccataag aactttgaat tttggatact
    12061 tttctggatt aaaattagca tagcttcgtt tagatataac aataaacgaa ctattaaaag
    12121 atttcatctt ctctattaaa tcatcctgtg ggattacctc acactcggga ttactaatag
    12181 tccaatcact atatcttgct aatccaaaga cgtcaccaat tgccatccct ttatagaagt
    12241 aaataccatc ctccaagcct aattggataa gattatctcc atataactca ctatattcac
    12301 ttgctttctt catcagttca tagctatagc gactttctaa gcgtgaatta aaattcaagt
    12361 attcataata aaaatatttg aattcaaaat gcaacgatcg ccccgaaaaa aaataataca
    12421 cacaaggata aaatatcgtc ccaacttgta atttgtaatg ttgatatctt ttattaaact
    12481 tgcaattgag atcgcaacca gcaatactga caatgggtaa atgggtgata aatacctgtc
    12541 accttgcgta acagaaaacc aaaataagac atatgttaaa acaatcgaga aaaataccca
    12601 aagtatttta ttattcctaa gtgtaaagac agacagtata gcataaaaaa caacatcact
    12661 gccaacttta ttaattgctt gttgcaaatc taatgtggca ggacttatac catgtcgagc
    12721 ttgttctgct gtttgaactt gtagatcaat ttcattccat aggtagtgac caaatatatt
    12781 tcctccagca ggactaaaag gatcaccaga gatccaaaaa ctacggacat accaataaat
    12841 gccaccaaga attgttgcca agctaaaaat aagaaatgtc tgtatgtttc tctttttcgc
    12901 tatcaatata acaaatatga tggctccata tatggcacca aatagtttta cgccacaagc
    12961 aattccaata aaaaaaccac ataaaaaaat taattcattt tgtgttttat tgttttctga
    13021 gcgaatatat ataagaatac tggctgcagc aaaacaaaga tagcgttaaa tcaatatatg
    13081 cataaccaag atgatcttta agtggattga tagtaaatag taggattgta gcaataatgc
    13141 taggataata agttttcgta atttcccatg aacagccaac aagtccacag gctattacaa
    13201 aaaaacagat atttgctaaa aactgagcta accttacatc tcctaactgt aaacctaaag
    13261 ccattaaaag atctgcattt tgcgggaaca gaggaaaacg caaatattgt tcaacaacaa
    13321 ttgcatggtg ctcaactaat gaacgagcca aaggtaagtg atacatagta tcatcccaat
    13381 caccgggagc tcgcagggac gataacaatg taaccacaaa aatcaaaaca agaaataaaa
    13441 taacaaacag cgtactgaac tcaatattat ttattgccag ccatctaaat tgctgacgtt
    13501 taataatgct taaaacccaa cacaccgtaa aaattaaaaa gatagcaatc ttattaaatt
    13561 gcccgaccat gcccagaaag taaattatca aaatatttaa agacatccct atgcataggc
    13621 aataaaaaaa taaagaaaat ttgttaagtc ctgccacccc ctccttaaaa aaagatgaca
    13681 atacaatatt accaatgaca aaaaatgtca ttccaaaagc aaaacaatac acaacataca
    13741 aatacaacgg caattcaatc atagtaaaat actctattta cattatcagt ttatagtttt
    13801 tttcagaaag ataaaataat gccttcattt cacgtctagt tgttgtattt gaatgcaaaa
    13861 taatgccaca tacaaatgcc attattgacg ataacatgat ggaggcagtt aaaaaggcag
    13921 tcggaaatct tctcaccaaa cctgtatcaa tataatcaac aatgatgggc aatcctaaaa
    13981 ttatagacaa agcagcaagt aaaacgccga taatactaaa aaagtatagt ggccgttcaa
    14041 gcgagtaaag tttaattatt gttttcagaa tcttgaaccc atcactccaa gttgatagtt
    14101 tgctcacact cccttcagat cgctcgccat attttgtatt aacctcacca tacttcatac
    14161 gtaattctaa agcatgaatt gtaagctcag tttcgatttc aaatcccctt gatagacatg
    14221 gaaaagattt aacatagcgt ctggaaaata cacgataccc cgacaacata tcagtaaaat
    14281 tccctttaaa gatagtctga acagtaccag tcaacagttt atttcctcag cgatgccctt
    14341 ttctgtatgt attttgatct ccattctctt gacgacatcc tacaaccata tccaattgat
    14401 tttttatgag atggttaatt atttctggag cagaacttgc atcataagtg ccatctccat
    14461 caaccattag ataaatatcc gcatctacat ctgagaacat acgtcttacg acattcccct
    14521 tgcctttcag aggaacagag tgaactgttg ctccagcatc ctctgccact tgtgctgtac
    14581 tgtcagtcga attattatca aatacatata tagaagcagt tggaatattc gttttgaaat
    14641 catttatgac ttttgctatt gccttctgct cattataaca aggaataatt acagcgatat
    14701 taaaagagtg atacataagc ccctcatata aactatttag ctaatgtgca agtaattgtg
    14761 tttttaacaa atcgtttact aacgaaaaat gaaatatttt gatgcgcaat atcctaaaac
    14821 caaactaaaa agagaaaagg tgaacaatgt aaatattgga gataaagcta agagatcaaa
    14881 taaaacgccg aataaataac tcatcgcccc cataaaaaat ataaatataa aatatctagc
    14941 cttggttgca gatactttaa atgagcatct tgcatttgca aaaaaagaaa aggttgctgc
    15001 acatataaaa ccaacaatgt tacttcggcc ttgaccaaaa gctaaattat tataacacgc
    15061 ataaaatacc ccccaatgta aagcagtatt gacaattcca attaaaaaat aactaaaaaa
    15121 agaactggtc aataacttcc ctatttttaa catcctttat tttgctccag aagtgaggtt
    15181 attactaatt tggatatttt ctatagaaaa tacccataaa actacaacgc ctataataaa
    15241 aattcccatg ggtgtaaaaa gaaatggatt agttatagaa acggtaatac tgaaaaaaag
    15301 tattaacgac atcattaatg atgtttttgt actatttaaa aaatagcctt tcaaaaagat
    15361 aagcaacata ggaactaatg atataaatac accaataacg cctgttttct gaagtatctc
    15421 aagaggtact atttcaatcc ttaatcgatc taatataaat gaaccaaatc ctcttccaaa
    15481 caagaacgtc gctaaatcaa catttttata ataaaaatat aaatcattaa atctaacaga
    15541 gtcagaatct tcactgcgtg atccgaccat gtaaagcata attataaata gaacgctgcc
    15601 caatattatt ataatagtat ttaaatatag cttatgtaat ttaaaactgg ctaataacag
    15661 tgataatgtt gtaaaaagta atacacctct tgattctgtc aataatatcg caagcactat
    15721 tatgagacat tttaaatatt ttttgtcgac aaaactgata atcgcaccga caccaaaaaa
    15781 gtagaaggat ttggaaaaaa aagccccatc gggacgaaaa aagaactcgc tagtcaatga
    15841 taagtgttca taaattaaat taaaatttaa caaaccgaaa ttcaacaaaa ttatgtatga
    15901 aatatataac agtgatccat ataatgcaaa tgcattaact attttaataa aaatctcaca
    15961 taaaaatact tttaaagtat atcttagtgt aaatagatat ataagtgaca gcataaaaag
    16021 gtatggttgc acatcaaaca gtatatcatt tattttatta ccagctaata agccgacaaa
    16081 gaagccataa aaaggaaata aaagcaacca tataaaaaga ctgacacatt tttgggtgat
    16141 tgaaaaattg aatgtaacta aattaaaaat taaaagtata aaactaaata caaataataa
    16201 atatcgcaaa gggaatattc ccaatggctc cagtaatctt ccaccaccac caataatcag
    16261 ttcaatacat aaaaatgtta taaaaatttt atttatatta ttcatattcg taaggtgatg
    16321 ttttttaaaa tatgtttaaa atcaacatta ataaaatata aattttatca gcctttttcc
    16381 gccttaactt ccaaaaactt aaagccaaga tcaaattgcc tatataacct ttacccaatt
    16441 gattattcac aaagctatca tcagctaagg cattagctat tttagttact tcttttcgat
    16501 accacttgtt tctcaccaaa ccaagccgtt tgtattttgc tttaaaacta atgtttgctc
    16561 ccacttggtt gttttcatgc tgtctgtaca gcattgttgg ttttctatca atataccacg
    16621 aataatttct cgtccgcgca aatgaatata aaaaccaatc atgcagacag atatcttgtg
    16681 cagcattttt attatttatg atgaacttct taaattcaat aagcgtttct ttttttataa
    16741 catatgtaca ccccggacct gctgcttcaa aaaaataatc gaattgagtt tgcgactgag
    16801 ccttatcaac taaatttttt tgccctgatg gataatatgc aattacattg ctggaataac
    16861 aattcgcatt attaaatacc aagcagtcaa ttgcattttt tattttatct ttataccaaa
    16921 tatcgtcctg atcactaaaa cagatataat catagttttc aagatcgaca tctttaatta
    16981 atctataaaa atttttacct gcccccccaa acttattgac gcttggaagt atatttataa
    17041 ttgagggata tgataattga aattcttcac aaatatttaa tgtattatca gttgataaat
    17101 catctgatat aaaaattgag atgtctacat ctttttgttc aataatactt tttaattgtt
    17161 cctcaatcca gcattcgcca ttatacgtag ccataagtac agctactctt ttttgatcag
    17221 aactatgcat tataacgacc gcccccagta attcctctta ttccatccag cgtagccaaa
    17281 atatgttttt tcatatattc aattttattt ttttgagttg cgataattaa agtcgtatga
    17341 attaaagaaa aaacaacttc cctaatagag agtaaaagag gcacatgatt ttttctaagc
    17401 aaaagaaatg catttcttac tcgataataa cgacgtactg gcgagtgaat tggtatgtta
    17461 atcccacaga tttttactga cttatcccca atagcatgaa ccatttctat atcaggaatg
    17521 atatgaacta atatgccata gtttaagcat ctaagacacc attctgtatc gacataatca
    17581 ataaaaagtt cctcatccat cattccaaca atctcaaaaa cttcttttga aaccatggtt
    17641 ccggatgaga ttgttactga acttttaaac ggtgtctgtc cttcagtgac atgaattttc
    17701 tctcgaagac catttttttt gatattacaa ataggataat aatgtgactt atctctatca
    17761 ataaaaactg ggccagctat ttttgcattt tcattatcca tatagcagga aagttttttg
    17821 ataaactcat tgcgaatacg gctatcttga tcaaaaaaaa ttatcttctc tgcccctttt
    17881 tcaagagaat atcgaatccc aatattttgt gcttttgcta taccttcatt cctgccaaga
    17941 caaattaaat gaactttttc aatgtctgct aatttatctt tcaaggtgaa attagtccta
    18001 ttatcgacta ctacaacgta acaattttgc tcctttagtt cagtgatcag tgcattcaga
    18061 tttttaagtt cgggattata tgtcacaatg acagcgtaaa tattactatt cattatccaa
    18121 gtgactcagt aattggttaa ttttagtttt catatacaga acacattcta gtataaagcc
    18181 attgtaataa cagagcatct aaaattacac gtatgctcca ggatattgcg gcacccagta
    18241 acccatattt ttcgaccata aaatacaaaa gggctaaata aggaaccaat tcagcacaat
    18301 gaatcaaagc agtaatttta gattttccta atgcctggat tgcagagaat ggtatcattg
    18361 ccagcgcatt gaagaaaaaa ccgatcaata acacatttaa tatattaaag gaattaattt
    18421 ggtaattctc accaaaccat attttcaata caagccctga atataacaag cctatgatta
    18481 tcaccggcaa acagattaga aacattaaaa gcaatgattt atttacatta cgtttaaatt
    18541 ctttaaagtc tttgatgtta cttaaccttg gaaacactgc cctcccgatt gctgcaggta
    18601 ttattcctaa ttttagaata acctccgctg gtgcagaata aaatgcaact ttatctgccc
    18661 ccataatatt tgacactata aatcgatcaa aatacaccat gaccggactt atgatattac
    18721 ttactgtcat ccaaccgcca aagaaaaaga gtcgtttaaa ggttttataa caaaaatgaa
    18781 cccccgaaat tttaatatca ttcctgacat aataggcaga gactaaaatc gaaataactc
    18841 ttgcaaatat taaaccagca accgctgccg acaatgtagc actataaaaa acaaatatgg
    18901 ccggaattcc cgcgatgcaa gagcttgata tagatttttg aatatttaca atgccaaatt
    18961 tttcatcccc ctcaagaatg gctgaccata attgatttag aataaataat ggaatgcaaa
    19021 tagccaacag tttaaatgct agttgtatat cactatgttc aacaccagaa atttttagat
    19081 aattaacaat tccatcagag aaaatcaata ataaaaaagc accaaagcat gaaaatagca
    19141 ccaaaaaaga tgtacttgtt gaaattacct tggttctttc atgatgatta tcacgatgaa
    19201 ttgcaatttc tctaataaca gagcgagtca ggcctacatc aaaaattcca gcatacccaa
    19261 caagtgcaat tgcaattgta taaacaccga aaccttcagg ccccaaacct cgagcgagaa
    19321 atcctagcga ggggatagca attaaagttg gtattgcata gccaaaaagg ttccagacac
    19381 tattttttat tatgctcatt attacatttc tttatcatat aaaataatca gcatctacaa
    19441 atttttgacc attaatatct ttatcagaaa gcaatggatt ttgaaccggc cattctatat
    19501 ttatactttt gtcgctccat aaaatcccac gctcttgcat ttttgaatag aaattatttg
    19561 ttttgtagac aaattctgct tcatcactaa gaacgcaaaa cccatgagcg aatccttccg
    19621 gtatccataa ttgacgctta ttctccgccg acaaatttac accaacccac ttgccaaatg
    19681 ttggtgatga tttccgaata tcaacagcta cgtcaaaaac ttcaccgaca acacagcgta
    19741 ccagttttcc ttgagcataa ggttccagct gatagtgcag tccgcgtaac acacccttag
    19801 ttgatttaga atggttatcc tgaacaaact caacctttcg tcctacagcc tcttcgaaaa
    19861 ctttctgatt gaagctttcc ataaaaaagc cccgctcatc accaaatact tttggttcaa
    19921 aaattagcac atcaggaatt tcagttttaa ttacgttcat tttattaata acctttaatc
    19981 attttcagca gatactgacc ataagcattt ttcttcagcg gctcggctaa taccttcact
    20041 tgctcagcat caataaagcc tttacggtaa gcaatttctt ccgggcagga aactttcagc
    20101 ccctgacgct cttcaattgt tgcaatgaag ttgcttgctt caataagact ttgatgtgtc
    20161 cccgtgtcca gccatgcata accacgcccc atcatagcga cagacaaacg cccttggtcc
    20221 atataaatac ggttaatatc ggtaatttcc agttcgccac gtgcagatgg tttaaggttt
    20281 ttcgccattt ctacaacgtc attgtcatag aaataaagcc cagtaaccgc atagttactt
    20341 tttggttcca gcggtttttc ttccagacta attgccgtac cgttatcatc aaattccacc
    20401 acaccgtagc gctcaggatc atttacgtga taggcaaaaa ccgttgcgcc actctctctg
    20461 ttaacagcgg tatccattaa tttcggcagg tcgtgaccat agaagatatt atcaccaagt
    20521 accagcgcac aatcatcacc acctataaat tcttcaccaa tgataaatgc ttgcgccaga
    20581 ccatccggac tcggttgtac tttatattgc agattaagcc cccactggct tccatcacca
    20641 agcaattgtt gaaaacgtgg tgtatcctgc ggcgtactga taatcagaat atctcgaata
    20701 cccgccaaca taagcgttga aagcggataa taaatcatcg gtttatcata aatcggtagc
    20761 agttgtttgc tcacagccat tgtcacagga taaagacgag tgccggaacc accagccaga
    20821 ataatacctt tacgcgtttt catttcacta ttccttttaa ttcatctcgc tctggcatca
    20881 tgagcgagat gcaataattt gttaaattgc cgtggtcgta aataattcgt tgagcatacg
    20941 tttcacgccc acctgccagt caggcaagac aagcgcaaag ttctgctgaa atttttcggt
    21001 attgaggcga gaattatggg gacgacgtgc tggtgtggga taggccgttg tcggcacggc
    21061 attaagtttg ttaagagcaa ggttgatccc cgctctgcgc gcttcttcaa ataccagagc
    21121 ggcataatcg tgccaggttg ttgtaccacc agcaaccaga tggtacaaac cagcaacttc
    21181 tggtttattt gcggccacgc gaatggcatg agcggtgcaa tcagccagta attcagcccc
    21241 cgttggtgcg ccgaattgat cgtttattac agccaactct tcgcgttctt ttgccaggcg
    21301 caacatcgtt ttggcaaagt tatttccttt accagcgtat acccagctgg tacggaaaat
    21361 aagatgcttt gcgcaatgtt cttgtaacgc tttttcccca gctagcttgg tttcaccgta
    21421 gacatttagc ggtgcggttg catccgtttc cagccatggc gtgtcaccgt tgcctgggaa
    21481 aacataatca gtggagtagt gtataaccca ggctccgact tcattagctg cttttgcaat
    21541 cgcttcgacg catgtcgcgt taagtaattg cgcgaaattc ggttctgatt ccgctttgtc
    21601 tactgcagtg tgagcagcag cattaacaat aacatcgggg cgaatttttt tgacggtttc
    21661 agccacacct tcggggttgc tgaaatctcc acaataatca gtggagtgaa catcgagcgc
    21721 aatcaagtta cccagaggag ccagagcacg ctgcagttcc caacccacct gccctgtttt
    21781 gccgaaaagc aggatattca ttactgacgg ccctcatagt tttgttcaat ccacgactga
    21841 taagcaccgc ttttcacatt ctcaacccaa tttgtattag ccagatacca ttccaccgtt
    21901 ttacgaatcc cgctctcaaa cgtttcttgc ggtttccagc ccaattcgcg gctaatttta
    21961 tcggcatcaa tggcgtaacg gcggtcatgc cccgggcgat cggcaacata agtaatttgc
    22021 tcacgataag atttctcttt cggtacaatc tcatccaaca aatcacaaat agtgagcact
    22081 acgtcgatgt ttttcttttc gttatgtcca ccaatgttat aagtttcacc cgctttacct
    22141 tcggttacga cggtatataa cgcacgagca tgatcctcta catacaacca gtcgcgaatt
    22201 tgatcccctt taccataaat aggtaatgcc ttaccttcca gagcattcag aataaccaat
    22261 ggaataagtt tttccgggaa gtgataagga ccataattgt tcgagcaatt agtcacaatg
    22321 gttggtaaac cataagtccg cttccaggca cgtaccaaat gatcgctgga agctttagac
    22381 gcagaatacg ggctacttgg cgcgtaagct gtcgtttccg taaatagcgg taacgcttcg
    22441 ttattatttg cttcatccgg atggggtaag tcaccataaa cttcatcagt agaaatatga
    22501 tgaaagcgga aacttttttt cttttcatca ttcagagcag accagtaatt gcgcgccgct
    22561 tctaaaagaa cataagtccc cacaatattg gtttcaataa atgccgcagg gcccgttatt
    22621 gaacggtcaa cgtggctctc tgccgccagg tgcatcaccg cgtctggctg gtgctgtgcg
    22681 aaaatacgcg acatggctac agcatcgcag atatctgcat gctcaaaagc ataacgttca
    22741 gaatcagaaa catcagcgag tgattctagg ttcccggcgt atgttaattt atcgacatta
    22801 acaacactat cttgcgtatt atttattatg tgacgaacaa cagcagaacc aataaaccct
    22861 gcgccaccag taacaagtat cttcactttt ttattccata tagctataga gcatgctgtg
    22921 aaatagactg ctccagattt gattaataga ggtattaata cacgctaccg cccctggctt
    22981 aacagctacc agtgcactgc atgcatctca acgatgtggc gagcgttagc cactcgcgcc
    23041 aaatccgaaa aattcaaacg ttaattgtct taccaaacag ccgtagaaac aaggaaaatc
    23101 ctggaaaaat ttgaataata tgagctatgt aacttcttgt ttttataggc tttataaaaa
    23161 cggcaacggc aggaaaattc aatgagcaaa acttatgcca cgaatcttca ctgccgttat
    23221 aattttctta tcaaccgtta catccggtca gattttcatt attcgcttaa cagcttctca
    23281 atacctttac ggaacttcgc cccttctttc aggttgcgta ggccatactt cacaaacgcc
    23341 tgcatatagc ccattttttt gccgcagtcg taactgtcgc cggtcatcag cattgcatca
    23401 acggattgtt ttttcgccag ctcggcaata gcatcagtca gctgaatacg tccccatgca
    23461 ccaggctgag tacgttccag ttccggccaa atatcggcag aaagcacata gcgacctacg
    23521 gccatgatgt ctgagtccag cgtctgcggc tgatccggtt tttcgataaa ttcaacaata
    23581 cggctgactt taccctcacg gtccagcggc tctttagtct ggatgacgga gtattcagag
    23641 aggtcacccg gcatacgttt tgccagcacc tggctgcggc ccgtttcgtt gaaacgtgca
    23701 atcatggcag caaggttgta acgtagcggg tcggcgctgg catcgtcgat cacaacgtct
    23761 ggcagtacca cgacaaatgg gttgtcacca atggcaggtc gcgcacacaa aatggagtgg
    23821 cctaaaccta aaggttcgcc ctgacgcacg ttcataatgg tcacgcccgg cggacagatg
    23881 gactgtactt ccgccagcag ttgacgcttc acgcgctgct caaggagtga ctctaactca
    23941 taagaggtgt cgaagtggtt ttcgaccgcg ttcttggacg cgtgagttac caggaggatt
    24001 tctttgatcc ctgcagccac aatctcgtca acaatgtact gaatcattgg cttgtcgacg
    24061 attggtagca tctctttggg tatcgcctta gtggcaggca acatatgcat cccgagaccc
    24121 gctacaggaa taactgcttt taaattcgtc attatttcat ccacctgtaa aatggttgct
    24181 gaattatagc ttgttcgatt tttttcgcca gcatcaatta ccctgaattg attactgaat
    24241 tacttgtgat gttacgccgc ttcgttgtgg attgcagtag cattgttcct aagtatggct
    24301 ccatttttcc aggaatggtc gcaaatctac tccctccgtt ccggcaatct aaagttaatc
    24361 ttttccacat taacaatatg gtgattaatc ctgtcgatat cgacggagct ttgccctctt
    24421 tcattcaccg catgaacatt tgcaagagac agcagtgttt cttttttcgc cataaagacg
    24481 ccgcgaacgt ctttgcgcat gtcgaagttc atgctcaatg ctggtccaac tgaggattcc
    24541 tgcatcacat tgatatttcg tataaaaaga tgttgcggct tgttgtgtaa ctccagcgag
    24601 gcacgcttca tctcaatgtt agttagcgac acaaaggaga cggcattccc ggcggagatt
    24661 tggatgccgc gcaatttata agcaagatgg gtattatcca gttgaatatt attcactcgg
    24721 aaattttgcg gtatcgagag atatttgcct ttaattaccc catagccgat taacatcccg
    24781 gcgctattaa tcatttcaat attatcaatc acgaaattgt cacaaccgta aatagcgact
    24841 gtcgcgttat caatgcccgc tttcttactg aaatccggcg tgatattgcg ggcattgata
    24901 ttacgaataa caaaatgttt accattttca acatggttca actgccgaca atccgatccc
    24961 gtgatattcg ccacgacaaa gtttttcact gcctggtctt ccgggtagtt gttatcataa
    25021 gtgcttcccg caaggcctat gccgatgccc cagttgattt tgccgttggt acagttgata
    25081 cgctcgatga catggtcaga tatcaaaata tcactgtcgt taattgccac gttccattca
    25141 atggcgtcgc cctgtaagtc gctgaactta cagttggtaa tgttggcacc gataatctgg
    25201 ttatgaaatc cctggcgtaa gatggcgtaa ttagcgtggc tgacagtgag gttatcgatg
    25261 gtcaggttgc gcatgacccg tttgttttta ccgccaatat aaatctgcat taccgggcca
    25321 aagccgctca tcgccagccc tttgatggtg cagtcagaac cacgcacatc cagggtaatg
    25381 ttatgcatac cgccgccctc tccccctgtc acctggctgc cgtcctgtaa gacaaatcgc
    25441 cctctgccgt tgccgcgcag gcttccaagg atgtgtaacg ttttgccggg agggatgaag
    25501 atgccggtgt tgatattgtc acaaacgaat ccggcaggca cgacgaccgt ttgcccttcg
    25561 ctgaaggctt gtttaaatga ggcgatccag tcatgtgggt tgtagtcgtt aatgttaacg
    25621 ctttgtcggg cgggaagtgc gcgagcgaaa ggggtatgga ggaaggcaag cgccgagctt
    25681 gccgtcagga aggtgcgtcg ggagagtgtt ttaaatggca tacgttctcc tctataaagc
    25741 ctgcagcaag ctggcgagtt ctcgattaat cacctgctgg ttaaaatcgt gttcgacttt
    25801 ttcgcgcgca cgtttgacga ccggagccag ttcgtcggta tccagttggc taaacgccgc
    25861 cagacgttgt gccagtgcgc gagcatcgtt ctcaggcacc agccagccgg atttatcggc
    25921 ctccaccagt tcaggaattc cgctatgcag agtagaaacc accggaatac cgaccgccat
    25981 cgcttccatt agcgccaccg gaatgccttc catatcgcca tccgcgcccg ttaccgatgg
    26041 caacaggaag acatccgcgt cgtcgagcat ctccttcact tcatggctcg gtttaaagcc
    26101 cggcatctct accacatctt ccagttgata ttgttcgatg agggtgcgca ggcgtcgttc
    26161 ccacaggcca atgccgagga tgcgatagcg aaatgccacg ccctgctctt tcaactgacg
    26221 gcaggcttca atcgccacat gcaggccttt tttctcggtt aagcgtgcga cagatatgat
    26281 ttccagcggc gttgcgggcg ctttcaccgg acgcgggcta aagcgcgtca tgtccacgcc
    26341 catacgcgat acggcgattt tttctctggg gcagcccatt ttttgcagcc ttccggccca
    26401 cagatcgctt atcggtaaca tcaggtcgcc acggcgaaac agttgttgat attcgggagt
    26461 gtagtggttg agcacttccc gactggagat atcaataccg tggaagatgg tggcaatttt
    26521 gccgcgaatg atacccagtt cgcgtagttt tgctgcggtt acccccgccg ggccaaaatg
    26581 agcgatgaac acatcggcgc gaaacggtgt tgcgacctga ccacaaatgg cagacaaaat
    26641 caggttccgc gactcggcac catagcgttt gaggttgagc gcatgccagg tatttttacg
    26701 atgaatgcca cgtaaggtct ggctggcgcg gtggcgcagt ttcgctactt tgcccgcagg
    26761 ttcgtcctgt aaccagcggg ttctggcgac aaggttgtat ttcgtccatg ccgcgtgggt
    26821 gttctctgta tcgccttttt gcaaagcgac aatctccact tcaaagccca tatcaataaa
    26881 cgcggtaatt tgattgagga cgaaggtttc cgacgacagc gggaatttca ataagaagaa
    26941 gccgaccttc atttcacctc cccgatgcgc tcaagcacag actgcaccat ctgcattccc
    27001 gtctgacgtt cgcgactgac ggcttcgtta aatcgagcgt tcagcaccgg aagctggcct
    27061 aaggtatccg caaccatcgc ttgcaggctg ccgtccagta aatgacggat atcaatcgcc
    27121 atctctggca gtcccagctg ctgcataatc ccggcggatt tatgttcata gttgatggca
    27181 atcgccggag tagcaaagtt cattgagata atggcggagt gcaggcgcgt accgacggtg
    27241 agttcacagg ctccgagaat tttgcccatt tccagatcgt tgagttcatc catcactacg
    27301 tggtaacggg caggatcgct gatgtgctgg cgcagattga gcgccaccat gcggtcgtct
    27361 ttgttatagc tgtcgatacc cgtacaggtg gagagcgcaa tcacctgata gccttcatcg
    27421 agaatgcgat tgaccacccc ggcaaaggct ttttcatacg cttgttgagt ggtgccgaga
    27481 cgtttgtcga acggtgccag ttcgcgcagg gtaattgcca ctgttttctg ttgtgcggca
    27541 acgtccagcc agtgttgaac ggcatagctg gcggtgaagt cttcggtgtg gtgatcgacc
    27601 agccacgcgg tatcgacgcc atgttccact tttgcggtgg tgatattgct gcgtttcatc
    27661 aaatcaaggc tgaccgattc gcgcaggatc agcgcgtcgc agtgaccaaa gacatagttc
    27721 gccagttggt taaattgctc atcctggaag gggccgacgc tgtgaccaat cataaacagc
    27781 ggctttttcg ccataaatgt gcaaagcgca tgttcaaact gcggcacgcc gtagagatcg
    27841 acaaaaaacg atccgccgac ctgaataatg gcgtcgtaac ctgacagcag gcgcacgaag
    27901 tcggtgaatc cctgggcgat ggcgatattg cgcagcttgc cagtgtcggt gacgcgtgag
    27961 agcaatacct gatgctggta gcggcgacgg aggacttttt taacgcgccc gacaacgccc
    28021 gccgcgctat tgtgttgtgt catctgcagg aacagcggat cgcccattac cgggcggttg
    28081 agcagccagg aagaactgac cggatagcgg ctcatcacgt ccacttcggt atgtggattg
    28141 aggatgttga tggcatcaag taagccgcgc aagatggcgc tgtcgccacg attgccgcaa
    28201 gtgtggttgc ccagaataag taatttcatt ttttcctcat aaatttgatg ccaggtgagg
    28261 ctgtgtttgt tgtgttcgtt gccgtttttt gcctgatgcg acgctggcgc gtcttatcag
    28321 gcctacaggt cccgagcaca gaaccgtagg acggataagg cgttttacgc cgcatccggc
    28381 aaccgttgtc ggaaccgaaa acagcaattc accccgcccg taaaagcatt ttcatttttt
    28441 cgctgcgaca aaactgacgc ttcacttcca ccaccagcgg atggcgtgac agcacaatca
    28501 tcaccacaaa cgccagcacg cccgcggcta tttgcaccgc cagcagcatc cccagcgcca
    28561 gttgcccttt cagcacaatg cccagcgcat aactgaccac cagcgtcggc aacgagagat
    28621 aaaacggcag ccacaaactc aggatgtact ggcgataact ggaaccaaga accggtttaa
    28681 tcatcacgaa gtaactcaga atggtgttga taatctgcac cagcaggaaa ccgagcgtga
    28741 cgccgattgc gcccgccatc tgcccaccaa taacaatcgc cggaataaac agaaatgttt
    28801 tgaatacatt gaatttaaag ctgatatcga cccgcgcttt cgccatcagc agcgaaccaa
    28861 tcgggttacc cacggagcgc agcagaccca caatacacag caattgcagc accggaataa
    28921 tgctgttcca cttttcgccg aataccagcg gtacgaagtt attcgacacc accatcagcc
    28981 ccagcagcgc cggaaagttg ataatcccca ctatcgacag cagcttgtag aagttaacac
    29041 gcagcttttc ggtgtcatcc tgaattttgg caaacgccgg aaacaacacg cgggtgatga
    29101 ttgggttcag cttcatcggc ggtacgacgg ccacgttgta cgccaggttg tatccccctg
    29161 ccacgcccgc gccaagaata cgcgccagca ccagcgttga aaggttggtg ttgagatagt
    29221 tgatgatgct gtccgccgtc agccaggcac caaaacgtaa gttcggtgcc acagacgcca
    29281 gcgagaagtg cagaccgggg caataaattt tgcgtccaaa gtagccaaac agcagtgttc
    29341 tcaccgcact attgaccaga taaccgagga tcgcggtcat cgccagcggc cagaaatggg
    29401 cgctaactac cgtaaaagtg aagcccgcca gcaccgcgct ggtttcgatc atgccgattt
    29461 tgttgaactc cagctccttc tgcatcaacg cgcggaactg ttgcccgtgg gggatcacca
    29521 caaacgccag cgacaatgtt ttaatcaacg gtgccaggtc cgggttattc agcacgtcgc
    29581 cgatgacatc actcaacaaa aacaccgcca cacataccac aagccccagc ccgacgttca
    29641 gccagtacag cgtggtgagt tcaaggtgac tgatttcttt tcgctgaata atcgagttag
    29701 cgataccgaa gtcagaaagc gtatctgcca gcgcgataat caccagcgac acagtcagca
    29761 ggccgaactg gtggttgtcg ataatccgcg ccagcacggt catctgcacc agcccgaggc
    29821 cgatgatgat caccgtggca atagccgacc acttcgcgcc gctgatggtt ttttcacgta
    29881 agctcatatc aatatgccgc tttgttaacg aagcccttga ataccgtcag gaaaacgatt
    29941 ttgatatcga accagacgct ccattcgcgg atgtactcaa ggtcgaactc gacgcgtttt
    30001 tccattttct ccagcgtgtc ggtttcgccg cgccagccat taatctgcgc ccagccggta
    30061 atgcccggtt tcactttatg gcgcagcatg tagccttcaa tgagctggcg atactgttcg
    30121 ttatgcgcta ctgcgtgcgg acgtggaccg acaatcgaca tccccccggt cagcacattg
    30181 ataaactgcg gcaattcgtc cagcgaggtg cggcgcagaa agttccccac tttagtgacg
    30241 cgcggatcgt tctgcgtcgc ctgggtcacc actttgtcgt tctccatcac tttcatggaa
    30301 cggaacttcc acactttgat cggcttgcca tccataccat agcgagtctg gcggaaaata
    30361 accggccctg gtgaactgag tttcaccgcc agcgcaatac agcacagcac cggggagatc
    30421 agcagcagaa taagcgtcgc cagcacaatg tcttccgcac gttttagcag gcggttaccc
    30481 cccgaaagcg gcgtgtcata cagcggcacc accggaacgc cgttcatctc ttcgagacgt
    30541 gaatggagaa tgttgaaggt aaagacgtcg gggatcagca gcaccgaaca ggtggtatcc
    30601 gccagttgat gggccagttt tttcactcgt gcgccgtcgc acatttgcat cgcgatatag
    30661 acgttatgaa tcttgcccgc ttttgcatcc tcgaccagct gttgcaggtt acccgcccag
    30721 tcgttagaaa cgccgcccgg tttcgggtcg tggtaaacgc ccaccacttc aaaccctaac
    30781 cacggctgat tacggaaact ctccatcagc atttgtccgg cggctaaatc ccccgctacg
    30841 gcgaccatcc gcttgttgta gccatgatta cgcagccagc ccgccccaat gcgaatacat
    30901 gaacggcaaa ccaccagtcc gatgctggtc agcccatacc acgccagcca gattttcagt
    30961 tgcgtgtcga aatcattgtt gaacgccacc agtccggcgc tgaaaatcac gcttaaggtc
    31021 cagttttgta gcaacagggc aaattctgtc gctgcccgaa caccgcgcca tgagcgataa
    31081 aaatcggtga tgccgcccag catctggaac accaccagcg taatcagcgc caccaacagg
    31141 tgcatgtaga ggaatgacag tccgctgact tcgcaaacca gccatagccc ggcaaacatg
    31201 atggtgatat ctgaaaagcg ttgcaccata gagattaacg atgcattggt tttcgctcgc
    31261 tcgcgctttt ttagatttgt catcgttgtt cctgttatta gccccttacc cgcagtgggg
    31321 taagggaaga tccgacatta ctcgttcagc aacgtcagca gagttcgcgt tcgcgcttcc
    31381 atcagcggca catcaccgcg cgattccaca ttcaggcgca ccaccggttc ggtattggag
    31441 gtgcgcaggt taaagcgcca gtcggcaaag gtcatgctga tgccatcgct gcgatccacc
    31501 gccagcgcct cacggctaaa atgctgttcc acgcggttaa tcgcctcaac gggttgcgcc
    31561 agtttgctgt tgatctcacc gcttgccgga aacgccgcca tccggtcgcg caccagttcg
    31621 cccagcgttt ttcctttcag gcacaccagt tcggcgacca gcagccacgg gatcatgccg
    31681 ctgtcgcagt aagcgaaatc acggaagtaa tggtgggcgc tcatttcgcc accgtagatg
    31741 gcgtcttcct tgcgcatccg ttctttaata aaggcgtgtc cggttttcga cattaccggc
    31801 gtgccgcctg cagcagtcac cacatcaata gtgttccagg agagacgcgg atcatggatg
    31861 atcttcgcgc cgggattttt ttcgaggaac gcttctgcca gcaggccgac aatgtagtag
    31921 ccctcgataa actgcccttt ttcgtcaaac agaaaacagc ggtcaaaatc gccatcaaag
    31981 gcaatgccca tatccgcgcc gtgtttgatg accgcattgc gggtgtcgtc gcgacattcc
    32041 ggcagcagcg ggttaggaat accgttgggg aaattgccgt ccggtgtgtt gtgtactttg
    32101 attaattcca cgggcgcgcc gagggcttta aagcgggctt caatggcgtc caccaccgga
    32161 cccgctgcgc cattcccgga gttgatcacc agcttgagcg gcgtgagatt cttgacattg
    32221 atataaccga acaggtgatc aacgtaagcg tcacgcaggt tgatttgctg atagcgaccg
    32281 cgtttggttt catcgacggg aggaaagtcg ttggcttcag ccagacgctg gacgtcgcgc
    32341 agtccggtat caccgctgat cgggcgagcc ccctcgcgca ccagcttcat gccgttgtaa
    32401 tccatcggat tatggctggc agtaacttca atgccgccat ccacgccgag atggaacgtg
    32461 gcgaaataga tctcttcggt gccggacata ccgatatcca gcacatcgac gcccgcatcc
    32521 tgtaaacctt ttgccagcgc cagttttaag gtttcgctgg tgagacggac atcaccgcct
    32581 aacacaatgg ttttcggttt gagaaattcg ccataggcgc gaccaatgcg ccaggcgata
    32641 tcttcattca gttcttcgcc taatttcccg cgaatatcat aggctttaaa gcaggttaat
    32701 tttttcatgt cgttaccctt tttcaggcaa tagttggccc tgaccgaaac ggccaatttt
    32761 atttttgtcg ttatttacac cgcggtttcg cattcattgc ctgatgcgac gtttacaccc
    32821 gtccgtagcg atccgcgaag cgcaccacat catcctcttc gagataagag ccggagcgca
    32881 cttcaattaa atcgagcgga attttccccg ggttttccag gcagtgcgtc gcccccagcg
    32941 gaatataaat ggactcgttt tcaccaagca gtttgatatc accatcaatg gtgacttttg
    33001 ccgttcccgc gaccactacc cagtgttccg cgcggtgatg gtgcatctgt accgacaaac
    33061 cctcgcccgg tttcacggtg atgcgtttca cctggtagcg gtcgcccgcg tcgatagagt
    33121 catatttgcc ccacggacga tacacttcgc gatgcacccg atgctcatgg cgaccatcgg
    33181 ctttgatctg ctcgaccact tttttcacat cctgtaccgc gttacggtcg gcaatcagca
    33241 ccgcatcttt ggtctgcacc actaccagat ctttcacccc gacggtggtg actaggcctg
    33301 attcggcata cacatagctg ttttcagttt tgtgattaat cacatcgccg tggcaaacgt
    33361 tgccctcggc ggtgtgggcg ctgatctccc ataatgaaga ccaggaacca acatcgctcc
    33421 agcccgcatc catcggcacc acaacagcat ctgccgtacg ttccatgacc gcgtaatcca
    33481 ccgactcttc cgggcaggcg agaaacgctt cttcatctac acgaataaaa tcgagatccg
    33541 gatcgacggc gctcatcgct ttttcgcagg catcgaggat atccgggcga tattttttca
    33601 gttcttcgag atagcgtccg gcgcggaaca ggaacatgcc gctgttccag taatattcgc
    33661 cgcttgctac ataggcctgg gcggtttcca gattcggttt ttcgacaaac tgcgccactt
    33721 caaaggccac cgtatcctgc tcacccgcag acacttcacc gcgacgaata tagccataac
    33781 cggtttctgg tagatccggc acaatgccaa aggtcaccag cttgcccgct tcggcataag
    33841 gcatggcatt acgcacggcg gcacggaacg cgtctttatc ggcaatcaca tgatccgccg
    33901 ccaataccag cattaacggg tcgctctccg ggctatgacg ttttgccgcc agcgccgcca
    33961 gtgcaatggc aggtgcagtg ttacgccctg ccggttcgag aataatgttc tcggtgagtt
    34021 tgttcagttg acgcagctgt tccgcgacaa taaagcggtg ctgctcattg caaatcacca
    34081 ccgggctttc gcactccacg ccgttcaggc ggcagatggt ggtttgcagc atggtgagat
    34141 cgcctttcag gcataaaaac tgcttggggt aaagtacgcg ggaaagcggc cataaacggc
    34201 taccggagcc acctgccatc acaactggat agagtttcga ctgcgccata attatccccg
    34261 aatatcattt ataaattgac gtaacacgtt ctctttatcg agcgtgcgtt cggcatattc
    34321 acgtgccacc gtgttatgtt tgggcagcag gagcgcctga cgaatccccg ccaccagcgc
    34381 ctcgaccgat tccggttcta cgcaaaccgc aatacccgga aaggtttcgc aaagctgccc
    34441 cagttctgtg tgggcctctg cggtaatcac cgcgttaccg cctaccgcca gaatattggt
    34501 cagtttcgac ggcaatacgg catctgccgc gccgcgtttt tacaccacca gatggcaatc
    34561 gcccatcttc agcagtgcgg gtaaagcgtc atacgattgc agcgggaaaa attgcatgtt
    34621 gcgcagtcca cgctgctgcg ccattttttc cagccgcgct ttgccgccgc cctgcccgac
    34681 aatggcaaaa agcagcggtt catcgcgcag gagatcggca gcttcaataa cgttttccag
    34741 cccctgcttt tcaccaatat tgccggagta aagaatgatt tttttgttat ccggcaggcc
    34801 aagctggtta cgaagggcat caacatcggc gtctgcaaca tgctgaaaac gggcaatttc
    34861 cgaccagttg gggaagaaga tgacgttatc cgccgccacg cctttttcga tggctttatt
    34921 catcatcgaa cgcgaaatcg tggaaacgtt atcgacgtta tgcagtccgc tacgttcgaa
    34981 cgccgttgcc agctgtgcca ctttgccgcc tttgcctttt ccggcaaggc ccagccccag
    35041 catggcgtcc acttcgtaat cctgaatatg cagcacggta cgcgcaccag agagcttcgc
    35101 cagcaggcgc attcccggcg tgcaaaacag cgttggcact acgccaataa tgcgatccgg
    35161 cttccagcga cgttgcgcca tcagtggaaa gaaactgctg acggcaaaac tgccgagatg
    35221 caacaagcgt ttcagggtgc tcggctgttt tggcacgtac agccaccgtg gcggcccctt
    35281 cttctcgttt gtagcgccag gcggaatagt tctcacccac ctgccattgc gggtagtaag
    35341 gcggtgcggt aatgacccgc acctcatgac cttgtgccgc caaccattcc accatctcgc
    35401 cggtgtattt gccgatgccg gttaactccg gcgagtagtt aatgccgtag accagtattt
    35461 tcataatccg ggtactccgg cacgcttctc agcgagaaaa taggcgcggc tgttagcgtg
    35521 aacatcgtta ctggcgagca gcgcgtccgg cgtcagccag cggtaatcgt catgctgctc
    35581 atccggcagt aacagctctt cttccgctac tctgaagcga aaaccgagca ccacatagtg
    35641 agtggtgaaa tccgtgccgg agaagttatc gtcataaaaa tgctgccaga caccgtaaaa
    35701 ctggcctgct gttatcggca gacgcagccc cagttccgcc atcgtcagcc gctcaaatgc
    35761 ggcttccagc gtttcgtctt tctgcacgcg ccctcccggc acaaaccagt aaccctgcgc
    35821 cgggcggttg gttcttttgc caagcagaaa ctcgccgcga ctgttctcga caataaagtc
    35881 gagagagaca agcggtgtgg agcgcactac agtggcaaag tcttcctgac gtaaaaacat
    35941 cattcccccc gaaagcggtc ttgattctca aggaaccact ggtaagtgct ggcaagcccc
    36001 gcttccagtg agatttcgtg ataccagcca agctgatgca ggcgcgtcac atccagcagt
    36061 ttgcgcggcg taccatccgg tttgctggca tcaaaaacca cccgaccttt gtaacccacc
    36121 actttggcga tggtttgcgc cagctcgcgg atagtgcagt caacgcccgt gccgacgtta
    36181 atgtgcgaca gcatcggttg gctattctcc agccagactt catgcgccag ctccatgaca
    36241 tgaatgctcg ccgccgccat atcatcgacg tgcaggaatt cacgcatcgg tgtaccgctg
    36301 ccccatacca ccacgtccgg cgcattctgt gccgtcgcct cgtggaagcg acgcagcaat
    36361 gctggtatca catgggagtt actcgggtgg aagttgtcat gcggcccgta caggttggtc
    36421 ggcatgacag agcggtaatc gcgtccatac tggcggttgt aagattcgca cagtttgatc
    36481 ccggcgattt tggcaatggc gtaaggctcg ttggtcggct ccagcgtgcc ctgcaataac
    36541 tcgctttctg ccatcggctg ttttgccagt ttcgggtaga tgcaggacga tccgagaaac
    36601 atcagtttgt tcacgtcgtt ctgatgcgcg gcgtgaatga tgttgctctc aatcatcatg
    36661 ttctggtaga tgaaatccgc cggataggtg ttgttggcaa caatgccgcc cactttcgcc
    36721 gccgccagat agacctggtc aatgcgttcg ctggcaaaga aatcatgcac ggcgcggctg
    36781 tccagcaggt tcagttcatc gcgggtacgt aataccagct ccacatcacc gcgctgttcg
    36841 agctgccgct tgatggcaga accgaccatc ccacaatggc cagcgataaa aatgcgttgt
    36901 ttacttatgc ttatgactcc agcgcgatcg ccacgtcgta gccgtgagat ttcagcagag
    36961 agtgtttttt cgccgcttcg aggtcattag ccaccatttc agacaccatc tctctgaggg
    37021 tgatttccgg tttccagccc agtttttcgt gcgctttggt cgggtcgccg agcagcgttt
    37081 caacttctgc cggacggaag taacgcggat caacggcgat aatcacatca cccggtttaa
    37141 cgcccggcgc gtcatgcccg gtgacggaaa ccacaatgcc cttctcttca acacccgtgc
    37201 cttcaaagcg cagtttgatg cccagctgtg ctgccgccat ttccacaaac tgacgtacgg
    37261 agtactgaac gccggtcgcg ataacgaaat cttccggctg ttcctgttgc agcatcatcc
    37321 actgcatttt tacgtagtct ttggcgtggc cccagtcacg cagggaatcc atatttccga
    37381 ggtacaggca cgactccagc ccctgggcga tgttggcgat tgcgcgggtg attttgcggg
    37441 taacgaaggt ttcgccgcgg cgcggggatt catggttgaa gagaattccg ttacaagcgt
    37501 acatgccgta ggattcgcgg tagttaacgg tgatccagta ggcgtacagt ttggcgaccg
    37561 catacggaga tcgcgggtag aacggcgtgg tttctttctg cggaatttcc tgcaccagac
    37621 catacagttc agaggtggaa gcctgataga aacgagtttt cttttccaga ccgaggaagc
    37681 ggatcgcctc cagcaggcgc agcgtaccca tcgcgtcgac gtcagcggta tattctggtg
    37741 actcaaaaga gaccgcaacg tggctcattg cgcccaggtt gtacacttca tccggctgta
    37801 cttcacgcaa aatgcgcgtc aggttagagg tatcactcag gtcgccataa tgcagatgga
    37861 atttcgggtt gcaggtgtgc ggatcctgat aaatgtgatc cacgcgctcg gtgttgaatg
    37921 acgatgcgcg acgcttaata ccatgcacct cgtaaccttt ttccagcaga aactctgcca
    37981 ggtaagaacc gtcttgtccg gttacaccgg tgatgagagc gacttttgac atgtcttatt
    38041 cctctgtatt tttgaattta ttcagtttca acgcgttcgc gtatcaccac tgcgggattc
    38101 ccccggcaaa ccacatttgc cggaagcgat ttaaaaacac tgcttcgtgc acccacgacg
    38161 gtgccgtcgc cgattgtgac gcctggggca acaaagacat cggttgccag ccagcatttc
    38221 tcgccaatca caataggcgt ggcgttaatg gtgaaatgtt gacttgcatg gtcgtggcta
    38281 ccggtgcata aataactttt ttgcgatatc accgaatgtg cgccaatggt tatttcaccg
    38341 agggtatata aattgacgtc atcgccaacc cacgcgtaat cacctaaggt taatttccac
    38401 ggataggtaa tttttactga cggacgaata actacgtttt ttcctatttt tgctccgaat
    38461 aaacgtaata aaaaagcccg ccagcgatac aatacttgtg gcgaccaggc aaatattgtt
    38521 gcctgtactg cccaccataa ttgcacttta atagcgttgc cgccccggaa ccctttcggc
    38581 accgagaaac cgcttaaatc ttgcattggt tttccttata ttcagacttt gttatataag
    38641 gctttagtct tacctgtcgt acgttggcgt aaatgccagg ataattctgc ccagaagcca
    38701 ggtacatgta atatctgccg ttggactttt ttggcgtcag cac
//
LOCUS       DAJTYG010000028        37867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTYG010000028 DAJTYG010000000
VERSION     DAJTYG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.028"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..2008
                     /gene="speF"
                     /locus_tag="OO568_001559"
                     /pseudo
     CDS             1..2008
                     /gene="speF"
                     /locus_tag="OO568_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2005..3324
                     /gene="potE"
                     /locus_tag="OO568_001560"
     CDS             2005..3324
                     /gene="potE"
                     /locus_tag="OO568_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6640958.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGTSLSPVQIGLATIGVQWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLPLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3380..5020)
                     /gene="pgm"
                     /locus_tag="OO568_001561"
     CDS             complement(3380..5020)
                     /gene="pgm"
                     /locus_tag="OO568_001561"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6640959.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5046..5591)
                     /gene="seqA"
                     /locus_tag="OO568_001562"
     CDS             complement(5046..5591)
                     /gene="seqA"
                     /locus_tag="OO568_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6640960.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5776..6540
                     /gene="ybfF"
                     /locus_tag="OO568_001563"
     CDS             5776..6540
                     /gene="ybfF"
                     /locus_tag="OO568_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6640961.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6680..6973
                     /gene="ybfE"
                     /locus_tag="OO568_001564"
     CDS             6680..6973
                     /gene="ybfE"
                     /locus_tag="OO568_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6640962.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7113..7643
                     /gene="fldA"
                     /locus_tag="OO568_001565"
     CDS             7113..7643
                     /gene="fldA"
                     /locus_tag="OO568_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6640963.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7853..7939
                     /gene="uof"
                     /locus_tag="OO568_001566"
     CDS             7853..7939
                     /gene="uof"
                     /locus_tag="OO568_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6640964.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7932..8378
                     /gene="fur"
                     /locus_tag="OO568_001567"
     CDS             7932..8378
                     /gene="fur"
                     /locus_tag="OO568_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6640965.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8462..8788)
                     /locus_tag="OO568_001568"
     CDS             complement(8462..8788)
                     /locus_tag="OO568_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6640966.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8825..10231)
                     /gene="chiP"
                     /locus_tag="OO568_001569"
     CDS             complement(8825..10231)
                     /gene="chiP"
                     /locus_tag="OO568_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR6640967.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSYFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYNDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10809..12473)
                     /gene="glnS"
                     /locus_tag="OO568_001570"
     CDS             complement(10809..12473)
                     /gene="glnS"
                     /locus_tag="OO568_001570"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6640968.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEIGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12676..14622)
                     /gene="nagE"
                     /locus_tag="OO568_001571"
     CDS             complement(12676..14622)
                     /gene="nagE"
                     /locus_tag="OO568_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6640969.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVIINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14955..15755
                     /gene="nagB"
                     /locus_tag="OO568_001572"
     CDS             14955..15755
                     /gene="nagB"
                     /locus_tag="OO568_001572"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6640970.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVVNSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15815..16963
                     /gene="nagA"
                     /locus_tag="OO568_001573"
     CDS             15815..16963
                     /gene="nagA"
                     /locus_tag="OO568_001573"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6640971.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPNFKITKTIVNGNEVVTQ"
     gene            16972..18192
                     /gene="nagC"
                     /locus_tag="OO568_001574"
                     /gene_synonym="nagR"
     CDS             16972..18192
                     /gene="nagC"
                     /locus_tag="OO568_001574"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6640972.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALKERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18240..18982
                     /gene="nagD"
                     /locus_tag="OO568_001575"
                     /pseudo
     CDS             18240..18982
                     /gene="nagD"
                     /locus_tag="OO568_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            19379..21043
                     /gene="asnB"
                     /locus_tag="OO568_001576"
     CDS             19379..21043
                     /gene="asnB"
                     /locus_tag="OO568_001576"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6640973.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21423..21499
                     /locus_tag="OO568_001577"
     tRNA            21423..21499
                     /locus_tag="OO568_001577"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21457..21459,aa:Met,seq:cat)
     gene            21508..21592
                     /locus_tag="OO568_001578"
     tRNA            21508..21592
                     /locus_tag="OO568_001578"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21542..21544,aa:Leu,seq:tag)
     gene            21616..21690
                     /locus_tag="OO568_001579"
     tRNA            21616..21690
                     /locus_tag="OO568_001579"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21648..21650,aa:Gln,seq:ttg)
     gene            21725..21799
                     /locus_tag="OO568_001580"
     tRNA            21725..21799
                     /locus_tag="OO568_001580"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21757..21759,aa:Gln,seq:ttg)
     gene            21815..21891
                     /locus_tag="OO568_001581"
     tRNA            21815..21891
                     /locus_tag="OO568_001581"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21849..21851,aa:Met,seq:cat)
     gene            21940..22014
                     /locus_tag="OO568_001582"
     tRNA            21940..22014
                     /locus_tag="OO568_001582"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21972..21974,aa:Gln,seq:ctg)
     gene            complement(22168..23343)
                     /gene="ubiF"
                     /locus_tag="OO568_001583"
     CDS             complement(22168..23343)
                     /gene="ubiF"
                     /locus_tag="OO568_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6640974.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            23489..24913
                     /gene="miaB"
                     /locus_tag="OO568_001584"
     CDS             23489..24913
                     /gene="miaB"
                     /locus_tag="OO568_001584"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6640975.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25066..26106
                     /locus_tag="OO568_001585"
     CDS             25066..26106
                     /locus_tag="OO568_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6640976.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAREEAEQKRKAAL
                     AAERKREEQEQK"
     gene            26103..26570
                     /gene="ybeY"
                     /locus_tag="OO568_001586"
     CDS             26103..26570
                     /gene="ybeY"
                     /locus_tag="OO568_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6640977.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            26660..27538
                     /gene="corC"
                     /locus_tag="OO568_001587"
     CDS             26660..27538
                     /gene="corC"
                     /locus_tag="OO568_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6640978.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFGMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            27563..29101
                     /gene="lnt"
                     /locus_tag="OO568_001588"
     CDS             27563..29101
                     /gene="lnt"
                     /locus_tag="OO568_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6640979.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            29498..30405
                     /gene="gltI"
                     /locus_tag="OO568_001589"
                     /pseudo
     CDS             29498..30405
                     /gene="gltI"
                     /locus_tag="OO568_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
     gene            30575..31315
                     /gene="gltJ"
                     /locus_tag="OO568_001590"
     CDS             30575..31315
                     /gene="gltJ"
                     /locus_tag="OO568_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR6640980.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTASRVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLT
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            31315..31989
                     /gene="gltK"
                     /locus_tag="OO568_001591"
     CDS             31315..31989
                     /gene="gltK"
                     /locus_tag="OO568_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6640981.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            31989..32714
                     /gene="gltL"
                     /locus_tag="OO568_001592"
     CDS             31989..32714
                     /gene="gltL"
                     /locus_tag="OO568_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6640982.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            32832..33767
                     /gene="rihA"
                     /locus_tag="OO568_001593"
     CDS             32832..33767
                     /gene="rihA"
                     /locus_tag="OO568_001593"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6640983.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTLGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            33851..35521
                     /gene="hscC"
                     /locus_tag="OO568_001594"
     CDS             33851..35521
                     /gene="hscC"
                     /locus_tag="OO568_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR6640984.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDKQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(35581..37032)
                     /gene="djlC"
                     /locus_tag="OO568_001595"
     CDS             complement(35581..37032)
                     /gene="djlC"
                     /locus_tag="OO568_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6640985.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIINRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(37029..37736)
                     /locus_tag="OO568_001596"
     CDS             complement(37029..37736)
                     /locus_tag="OO568_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6640986.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         9259 a         9686 c         9587 g         9335 t
ORIGIN      
        1 ggaaaatgaa gaacgtgtac cagcagaata tctgccacgt atttctggtg tcttcgagca
       61 ttgcgaatca cggaaagagt tttatggacg tcagttagaa accgctgcta gccattatga
      121 aacgcaactg cgcccgccct tcttccgcgc actggtcgat tatgtcaatc agggcaatag
      181 cgcttttgac tgcccgggtc attagggagg tgagtttttc cgtcgtcatc cggcagggaa
      241 tcagttcgtg gagtacttcg gagaaacgct gttccgttcc gacttatgca acgcagacgt
      301 agcaatgggc gatctgctga ttcacgaagg cgcgccatgc attgcacagc aacatgcggc
      361 aaaagtgttt aatgccgata aaacatactt cgttctaaat ggcacttcat cttctaacaa
      421 agtggtttta aacgccctgc taacaccggg tgatctggtg ctgtttgatc gcaataacca
      481 caaatctaac caccacggag cgttgctaca ggctggtgca acaccggttt atctggaaac
      541 ggcacgtaac ccgtatggct ttatcggtgg cattgatgcg cactgttttg aagaaagtta
      601 cctgcgtgag ctgatcgcgg aagtcgcacc gcagcgggca aaagaggctc gtcctttccg
      661 cctcgctgtg attcagttag gcacctacga cggtacgatt tataacgccc gccaggtggt
      721 ggataaaatt ggtcatctgt gtgactacat tctgtttgac tccgcatggg ttggctatga
      781 gcaatttatc ccgatgatgg ctgactgttc gccgctgttg ctggatctta atgagaacga
      841 tccgggtatt ctagttacgc aatccgtaca taaacagcag gctggttttt ctcaaacttc
      901 acaaatccat aaaaaagaca gccacatcaa agggcaacag cgttatgtac cgcacaaacg
      961 catgaacaac gcctttatga tgcacgcctc caccagcccg ttctatccgc tgtttgccgc
     1021 actggatatc aacgccaaaa tgcatgaagg tgtcagcggt cgtaatatgt ggatggattg
     1081 tgtggtaaat ggcattaatg cccgcaaact gatcctcgat aactgtcagc atattcgtcc
     1141 gttcgtacct gaactggtgg atggtaaacc ctggcagtcg tatgaaacag cgcaaattgc
     1201 ggttgatctg cgcttcttcc agtttgtacc aggggaacac tggcattctt ttgaaggcta
     1261 tgcagagaat caatactttg tcgatcctta caaactgttg ctgacaaccc caggtattga
     1321 tgcacgtaac ggcgaatatg aagcgttcgg tgtacccgcg acgattcttg ctaacttcct
     1381 gcgcgaaaat ggcgtagtgc cggaaaaatg cgatcttaac tccatcctct tcctgctgac
     1441 tccggcagaa gatatggcca aacttcagca acttgttgcc ctgctggtac gcttcgaaaa
     1501 actgcttgag tccgacgcgc cattagcaga agtgctgcct tccatctaca aacagcatga
     1561 agagcgctac gccggttata ccctgcgtca gttgtgccag gaaatgcatg atttgtatgc
     1621 ccgccacaac gtgaaacaac tgcaaaaaga gatgttccgc aaagcgcact tcccacacgt
     1681 cagcatgaat ccgcaagaag ccaactacgc ctatttacgc ggtgaagtgg agctggttcg
     1741 tctgccggat gccgaaggtc gtatcgctgc cgaaggggcg cttccttatc ctccgggtgt
     1801 gctgtgtgtt gttccgggtg aaatctgggg tggtgctgtt ttgcgttact tcagcgctct
     1861 ggaagaaggg atcaacctgc tgccaggttt tgcaccggag ctgcagggtg tctatatcga
     1921 agaacatgat ggtcgtaagc aagtttggtg ctatgtcatc aagcctcgtg atgcgcaaag
     1981 caccctgttg aaaggggaaa aattatgagt caggctaaat cgaacaaaat gggcgtcgtt
     2041 cagttaacca tactgacgat ggtcaacatg atgggctccg gtatcatcat gctgccgaca
     2101 aagcttgccg aagttgggac aatttcaatt atctcctggc tggtgacagc cgtaggctca
     2161 atggcactgg catgggcatt cgcaaaatgc ggtatgttca gccgtaaatc aggtggtatg
     2221 ggcggttatg ccgagtatgc tttcggtaaa tccggtaact ttatggcgaa ctatacctac
     2281 ggcgtctcac tgctgattgc taacgtcgcg attgctattt cggcggttgg ttacggcacc
     2341 gaactgctcg gcacgagttt gtcgccagtg cagattggtc ttgcgaccat cggggtgcag
     2401 tggatttgta ccgtggctaa ctttggtggt gcgcgcatta ccgggcaaat cagtagcatt
     2461 accgtgtggg gggtcattat tccggtggta ggcctgtgca ttatcggctg gttctggttt
     2521 agcccgacgc tttacgttga ttcctggaat ccgcatcatg caccgttctt cagtgcagta
     2581 ggttcttcca tcgctatgac gctgtgggct tttcttggtc tggagtctgc gtgtgcgaat
     2641 actgatgtag tggaaaaccc ggaacgtaat gtgccaatcg cggtactcgg cggtacgtta
     2701 ggtgcggcgg tgatttatat cgtctccacc aacgtgattg cagggattgt gcctaatatg
     2761 gagctggcaa attcaacggc accatttggc ctggccttcg cacagatgtt cacaccagaa
     2821 gtgggtaaag tcattatggc gctgatggtg atgtcctgct gcggttcgct gcttggctgg
     2881 cagttcacca ttgcccaggt gtttaaatct tcatctgatg aaggctactt ccctaaaatt
     2941 ttctcccgtg taaccaaagt ggatgcaccg gtgcagggaa tgttgaccat tgtgattatc
     3001 cagagcgggt tgccgctgat gacgattagc ccgtcgctaa acagtcagtt caacgtgctg
     3061 gttaacctgg ccgtggtgac caatatcatt ccgtatattc tgtcaatggc ggcattagtc
     3121 attattcaga aggtcgctaa tgtgcccccc tcaaaagcga aagttgcaaa ctttgttgct
     3181 tttgttggcg cgatgtatag cttttatgcg ctgtactcat ccggggaaga agccatgctg
     3241 tacggttcca tcgtgacctt ccttggttgg acactgtatg gtctggtatc accacgcttt
     3301 gaactgaaaa ataaacacgg ttaaaaaagc aaagtaaaaa agggcgatct tgcgaccgcc
     3361 ctttttttat taaatgtgtt tacgcgtttt tcagaacttc gctaacaatc tcaaccgctt
     3421 ctttctcaat ctgcttgcga tgttcttcac cgaggaagct ttcgcagtag atcttatatg
     3481 cgtcttccgt gcctgacgga cgcgcggcga accagccgtt gtcagtcatc actttcagac
     3541 cgccaataga agcaccgttg cccggagcag cagtcaggcg cgcggtgatc gggtcacctg
     3601 ccagggtgct ggcgctcacc atttccggag acagcttaga cagcgccgct ttttgtgcgg
     3661 aagtcgcagc tgcctgcaaa cggttgtagc tcggcgcacc aaagcgtttt gccagttcgt
     3721 tgtagtgttc ctgcgggttc ttaccggtga cagcggtgat ttccgccgcc agcagacaca
     3781 taatgatgcc gtccttgtcg gtggaccacg gcgtgccgtc gaaacgcagg aaggaagccc
     3841 ctgcgctctc ttcgccgcca aagccgaagc tgccgtcgaa cagaccatca acaaaccatt
     3901 tgaaacctac cggtacttct accagcttac ggcccaagtc attgaccaca cggtcgatca
     3961 tcgcagaaga aaccagcgtt ttaccgacag caacatcttt gccccactgc ggacgatgct
     4021 ggaacaggta gttgatcgcc accgccaggt agtggttcgg attcatcaaa cctgccggag
     4081 tgacgatacc gtgacggtca taatccgggt cgttagcaaa cgccagatcg aacttatcac
     4141 gcagtgccag cagacccgcc atcgcacact cggaggagca gtccatacgg atcgcaccgt
     4201 ctttatcgag gtgcataaag cggaaggttt gatcgacctg atcgttaacg atggtcaggt
     4261 tgaggttgta atactcgcca atgcgcttcc agtattcgat accggaaccg cccaacggat
     4321 caacgcccag cgtcaggccc gctttctgaa tcgccgccat atcaacgata tcggccagcc
     4381 cttccacgaa cggctgcacc agatcctgct ctttcacata gccggatgcc atcgcttcgt
     4441 cgagggagat acgcttcacg cctttcaggc catcggccat cagtgcgttg gccctgtctt
     4501 ccaccacttt ggtgacgtta gtatcagccg ggccaccatt tggcggattg tacttgatac
     4561 caccatcttc cggcgggtta tgggacggtg taatcacgat accgtctgcc agcgggccac
     4621 cttttttatt gtgaaccagg atggcattgg aaacggcagg cgtcggagtg aagccattgt
     4681 tttcttgcac aatgacatca acgccgttcg ctgccagcac ttccagtacg gaaatgaacg
     4741 caggctcgga cagggcgtga gtatctttac ccacatagca agggccagtg atgccgtttt
     4801 tcgcacgttc ttcagcaatt gcctgagcga tcgccagaat gtgcggctcg ttaaagctgt
     4861 ggcgcgctgc actgccacgg tgaccggaag taccgaattt caccgcgtgc tccgcattcc
     4921 ctgcttctgg tttcagtaca taatattgcg ccgtcagttg ggcgacgtta atcaaatcac
     4981 tctgttgtgc aggttggcct gcacgattgt ggattgccat tgctttgtcc tttgtctgca
     5041 acgttttaga tagttccgca aaccttctca atcaattccg ccgggaattg catcgactgc
     5101 atgatgtgtt cgatcatgct gcatttacgg ccggtgttgg tgttggtgat cacccaatac
     5161 ggcgtgcctg gcacatgttt cggcttggtc tgattaccat ttttcagcag cgtttgttca
     5221 tctgccgcaa agtacacgcg tgtacgaccg tgcaacgatt ccgttgcttc ggcaaacgcc
     5281 tgggcgtcaa gagaatatag tgtagacaac agcagcataa agcgattgac cgctcgcttt
     5341 tgctctgcgt attcgtccga aagcagaagt tcacgcattg cgcgaacctt gtctttaatc
     5401 gttttgaccg gcttcgcttc gacgatagca ggtgacgcaa cgcgaacctc tttcgtcacc
     5461 ggagcagcag gctgtgatgc ggcggaaaat ttcaacatac gccgtaaaat gtcggatgcg
     5521 ctctcgccga tatgcttagt gtggctggca atatagctgt agagttcatc atcaacttca
     5581 atcgttttca tcttaatcca gtgcagtgtc ttagctgaat acaagtcgcc aagagtatat
     5641 gaataaatcc cggcagcgga tagcgtcaag cctggcttgc agaaaaactc ggaataaacc
     5701 ttatttgttg cagccctgtg gcacaatggt caacataata ccctaacccg acaacgcgaa
     5761 aaaaagaact ttgccatgaa attaaatatc cgcgcgcaaa ctgcacaaaa ccagcacaat
     5821 aattctccca tcgttcttgt ccatggtctg tttggtagcc tcgacaacct tggcgtactg
     5881 gctcgcgatc tggtaaacga tcacaatatc atccaggttg atatgcgtaa ccacggtctt
     5941 tcaccaagag agccggtaat gaattacccg gcgatggcgc aggatcttgt tgataccctg
     6001 gatgcactgc agatcgacaa agcaacattt atcggtcact ccatgggcgg taaagcggta
     6061 atggcactta ctgcactggc ccccgatcgc atcgataaac tggtggcgat cgatatcgca
     6121 ccggtcgact atcacgtacg ccgtcatgat gagattttcg cagctatcaa cgcggtcagt
     6181 gaatcggacg cacaaactcg ccagcaagca gcagcaataa tgcgccagca tcttaatgaa
     6241 gaaggggtga ttcagtttct gttgaaatct tttgttgacg gggagtggcg ctttaacgta
     6301 ccggtattgt gggatcagta tccgcatatt gtgggttggg agaaaatccc ggcatgggat
     6361 caccctgccc tgtttatccc tggcggcaat tctccgtatg ttagcgagca gtaccgtgat
     6421 gatttactgg cacaatttcc acaggcacgg gcgcatgtga ttgcaggcgc gggtcactgg
     6481 gtccatgctg aaaaaccgga tgcggtatta cgcgctatcc gtcgctatct caatgattaa
     6541 cgcaactgat taaaaaccca gcgtccgcgt cgctttccgc gcgtgggcgt tggcgtgact
     6601 catcgcgctg atgtattatg gcgcgctatc catccgggca gaggcctggc tgattgtttc
     6661 ccccgaagtc accaagatca tggccaaaga acaaacggac cgtacgacat tagatctgtt
     6721 cgcgcacgag cgtcgaccgg gacgaccgaa aactaatccg ctttcgcgcg atgaacagct
     6781 gcgtattaat aaacgcaacc agctaaaacg cgacaaagta cgtggcctta agcgtgtcga
     6841 actgaagctg aacgcggaag ctgtcgaggc gctgaacgag ctggcggaat cgcgtaatat
     6901 gagccgtagc gaactgatcg aagagatgtt aatgcagcaa ctggcggcgc tgcgtagtca
     6961 gggcatcgtt taaatttcca ctttcatgta gcacagtgtg cagtcctgct cgtttgctga
     7021 ttccgcaaga ctgcctgttc tgctatgatt gcctttatcc gtgggcaatt ttccaccccc
     7081 atttcaataa gtttcaagag gttatttcac tcatggctat cactggcatc tttttcggca
     7141 gcgacaccgg taataccgaa aatatcgcaa aaatgattca aaaacagctt ggtaaagacg
     7201 ttgccgatgt ccatgacatt gcaaaaagca gcaaagaaga tctggaagct tacgacattc
     7261 tgctgctggg catcccaacc tggtattacg gcgaagcgca gtgtgactgg gatgacttct
     7321 tcccgactct cgaagagatt gatttcaatg gtaaactggt tgcgctgttt ggttgtggtg
     7381 accaggaaga ttacgccgaa tatttctgcg acgcattggg caccatccgc gacatcattg
     7441 aaccgcgcgg tgcaaccatc gttggtcact ggccaactgc gggctatcat ttcgaagcat
     7501 caaaaggtct ggcagatgac gaccactttg tcggtctggc tatcgacgaa gaccgtcagc
     7561 cggaactgac cgctgaacgt gtagaaaaat gggttaaaca gatttctgaa gagttgcatc
     7621 tcgacgaaat tcttaatgcc tgatgtgatg cggcgtagac tcatgtctac gccgtattaa
     7681 tagataatgc caatcaaaat aattgctaca aatttgtaac ttttgctgtt gtacctgtac
     7741 aatgtcccag tgttcaagtg gccttgccgt tgtaaatgta agctgtgcca cgtttttatt
     7801 aacaatattt gccagggact tgtggttttc atttaggcgt ggcaattcta taatgatacg
     7861 cattatctca agagcaaatt ctgtcacttc ttctaatgaa gtgaaccgct tagtaacagg
     7921 acagattccg catgactgat aacaataccg ccctaaagaa agctggcctg aaagtaacgc
     7981 ttcctcgttt aaaaatcctg gaagttcttc aggagccgga caaccatcac gtcagtgcgg
     8041 aagatttata caaacgtctg atcgatatgg gtgaagaaat tggtctggct acggtatatc
     8101 gcgtactgaa ccagtttgac gacgctggta tcgtcacccg ccacaatttt gaaggcggta
     8161 aatccgtatt tgaactgaca cagcaacatc accacgatca cctgatttgc ctcgactgcg
     8221 gcaaggttat cgaatttagt gatgattcca tcgaagcgcg tcagcgtgaa attgccgcaa
     8281 aacatggcat tcgcctgact aaccacagtc tctatcttta cggtcactgt gccgaaggcg
     8341 attgccgcga agatgagcac gcgcacgaag gcaaataagc cagcctgaac gagaaaagcc
     8401 aacctgcggg ttggcttttt tatacaaggg aaataagaaa agcgagagtt acagctctca
     8461 cttatttgtt attactgcgt atttcctgcc agaccttatc acaatcggct ttcaccgcct
     8521 gatcatttcc ggtttgcgtt gcacgcagac actgctgata atccagtacg cgtacacttt
     8581 cctggtcggc aaactgctga tgttgtttct ctttcttcag cacgtttaac acgctctgac
     8641 aggcttcaat tttttctggc gaaccttgcg cggtgttgat acaggcgcta tatgcctctt
     8701 tcagacggct gtcttcctgt ggtgcggttg attgcgcaca agctaccagc cccgatgcca
     8761 tcatggcgat gagaatcagt tttttcattg aatgcggcag gcacaaaacc tgccgcgtcg
     8821 ggcatcagaa gatggtgaat ggtgcgatta ccataaattt cacgtcacgc tcatcctgga
     8881 agatgttacc gtaaccgccg ccccagcttg ggatgtcgga gtggttgtcg tattcggtga
     8941 agtgcagttt gaacatcgtg cctttggcgc gaccgtcctg aatggtgtag accgcatcca
     9001 ggctgtaggc agactcttca atagtccggt ttttgtcgtt gtacgcatcc ggattgctct
     9061 gccaggtcgc aggtttagca tcccatgcgt aaacgtagga agcgccgatg gcgaagcctg
     9121 gaagattcca gtttttcagg tcatacatcg caccgaagaa gaccgctttt tcgccgttag
     9181 cgttgaagta ggaacggtta tcccaccaga tatccaggcg gccgttggag gaagcgtagg
     9241 ttggggtcat acgttgcagg aagtatccct gctgactgtc agccttaacc caggtgcctt
     9301 cgaggcgcaa atccactacg tcagccgccc ggtaaccaaa ggtcaacgct tgcagccaag
     9361 cggtgccgtc ataaagatcg ttgacgctgc gatcgtcaac tttatcgcgg gtaccataga
     9421 actggtagct ggtggttaac gggctaccgg cgatatcaaa tttgtagctg gctttggcaa
     9481 aatattgatc gatataccct tccgcctgac caaatgccgc ttccagtacg aagttatttt
     9541 tgaagtcgta tttcgcaccg atggagtgca gataatcaac tttggtggtt ttatcgttct
     9601 gataaaactc atccatttcc agatgccacg gcgctttgta ttcgttggtc cacatgtagg
     9661 agaaactcaa ggcaccagca tcgccgtaat caaaattcgc cccggcttcc gccccctgat
     9721 aagtacccgg cataaagctc cagtgcggcg ctaacagcgt ttgaccggtt ggctgaatgt
     9781 aacctgccct cgcccaaacc ggaccgtatt taaatttggc cgcagcttta tacaggctta
     9841 taccgctttt gtcgccggac cagtcttcgt cataggcttt attacttttt gaaaacgcga
     9901 tttcgttcgg gtgggagctg tcgccgtttt ccgccatttc aatcgccgta aacgcagcaa
     9961 tatcaaggcc gaacatatca gcagcataac cggactgaaa atcgaggttg gcattccagg
    10021 tggagtgaga aaggttggtt ttgtatttgt cgccgtcggt aacatcttta cggtcgcgtt
    10081 cacgctgcca gtaatagata ccgccggtta aggttgaatc gtcgatgaat ccttcggcgc
    10141 gagcctccgg cattgccata aagcccgaca ttgctgtaac accggcaata gccagcgcca
    10201 gcgtactacg tttgccacta aacgtacgca tgggttaatc ctctttgacg tataaattgc
    10261 tgcaccaaag gtgaacagcg aaaaaaataa aaattaaaaa agtagcgttg agaaaagtct
    10321 cgctgactac aggaacagac tatttttcgc gacgcgaatt atcaatctct ttctgtaacc
    10381 agaatgtaag aacatgagca accgcacata ttttgatgat ttttgttaca aggcttaagt
    10441 ttgcggttga aaggcttgca gaagaataaa aacgtgaaat tccagcaaga tgaaagcggt
    10501 tacattttat ttaattatgt tttgtgattt taaatttatt ttatggataa attgcgaagc
    10561 ggaattaatt cgcgaagaaa agcagaaaaa acgccgcaga gcgcggcgtt tgggattgtt
    10621 tgctatttct tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag cacgcaaatt
    10681 caatatattg cagagatcat gtaggcctga taagcgaagc gcatcaggca atttagcgtt
    10741 tgaaatttat tgtctattta ttgccggatg cgacgtaaat gcctcacccg gcttggtaga
    10801 acttaaaatt actcgcctac tttcgcccag gtatcacgca gcccaacggt gcggttaaat
    10861 accggttttt cggccgtgga atggcggctg tcgaggcaga agtaaccttc acgctcaaac
    10921 tggaatgctt tacccgctac cgcatctttc agcgacggtt cagcaaagcc ctgtttgatc
    10981 accagcgatt ccgggttaat caccgacagg aaatcatccg cagcacctgg gttaggcacg
    11041 ctgaacagac gatcatacaa acggatttca accggcagcg catgtgccgc gctcacccag
    11101 tgaataacac ctttgacttt acgaccatct gccggatctt tgcttaaggt gtcggcgtca
    11161 taagtacaga agatggtggt gatattacct tcggcatctt tctccacgcg ttcagcctta
    11221 atgacgtaag cattacgcag acgcacttct ttacccagca ccagacgttt gtactgcttg
    11281 ttagcttctt cgcggaaatc ggcgcgatcg atccaaattt caccgctaaa cggcacctga
    11341 cggctcccta tttccggttt gttcggatgg ttcggcatgg tgaccatttc gccttcgccc
    11401 tgatagtttt cgataaccag tttcaccgga tcgataactg ccattgcgcg cggcgcattt
    11461 tcgttgagat cttcacggat gcaggattcc agcgacgcca tctcaatggt gttgtcctgc
    11521 ttggtcacgc cgatgcgttt gcagaactca cgaatagaag ccgcagtgta accacgacga
    11581 cgcagaccgg aaatggtcgg catacgcggg tcatcccagc cttcaacgtg cttgtcggtc
    11641 accagcaggt tcagcttacg cttggacatc acagtgtatt ccagattcag gcgcgagaat
    11701 tcgtactggc gcggatgaac aggaatggtg atgttatcca gtacccagtc gtacagacga
    11761 cggttgtcct ggaactcaag cgtacacaga gagtgcgtaa taccttccag cgcatcgctg
    11821 atgcaatggg tgaagtcgta catcgggtag atgcaccact tgttgccagt ctggtggtgt
    11881 tcagcaaatt taatgcggta cagcaccgga tcgcgcatta cgataaatgg cgacgccatg
    11941 tcgattttcg cacgcaggca ggctttacct tcttcaaaac caccggcacg cattttttcg
    12001 aacagcgcca agttctcttc aacgctgcgg tcgcggtacg ggctgttttt acccggctgc
    12061 gtcagggtac cgcggtattc gcggatctgt tccggtgtca gttcatcaac gtacgccagg
    12121 cctttattga tcagttcgat cgcataggcg tggagctgat caaaataatc ggaggagtaa
    12181 cggacgttac cagaccagtg aaaacctaac cactctacgt cgtttttgat cgaatcgaca
    12241 tactcgatat cttcttttac cgggttagtg tcgtcgaaac gcaggttgca ctggccttta
    12301 tagtcctggg cgatcccgaa gttcaggcag atagatttcg catggccaat atgcagatag
    12361 ccattcggct ccggcgggaa acgggtgtgt actgtggtgt gcttaccact ggccagatct
    12421 tcatcgatga tctgacggat aaagttagtc gggcgggctt ctgcctcact catcgtggat
    12481 tcctcaaagc gtaaacaacg tataacggcg tatgatctta taagcgggac aggctgacaa
    12541 ctgttagttt tttaaaattg ataatctaac aaggattatg gggatgattg atgaaaaaaa
    12601 aagcggcaga gaagatctcc accgcttgtc attgttggat gcgacgctgc cgtgtcgcat
    12661 caggcataca gcagattact ttttgatttc atacagcggt gtttgacccg ccacaacatg
    12721 gccctgagct ttaatgatca agccactgaa atcgtcgata ttgctgcaaa ccaccgggct
    12781 aatcatcgag cgggcgttag cgttcaggta atccagatcc atttccagaa tcggttgccc
    12841 tgcgcttacc tgcgcaccct cttccaccag acgtttaaag cctttacctt ccagcgctac
    12901 ggtgtcgata cccatatgga cgacgatctc cgcgcctttt tcggtttcca ggcagaacgc
    12961 gtggttggtg ttgaagattt tcacgattgt ccctgcggct ggtgatacga cgattttatc
    13021 tgtcggtttc accgccacac cgtcacccac cgctttgctg gcgaatgctt cgtcaggaac
    13081 ctgatccagt gccacgacat caccggtaat cggcgatacc agctccgcga tagataccgc
    13141 gtttggtaca gcctgcggtt ttgctacagg cgcggcagtt gccggagttg cttcagctga
    13201 cgcagcggct accggaccac gggcaacgac tttcttcatc gcatcgccga tggattctgc
    13261 tttcgcgcca acaatcacct gaatagtctg tttgttcagt ttcactacac cagaagcacc
    13321 cagacgttta cacatcgtat cgttgacgcg ggcagagtca gccacggtca ggcgcagacg
    13381 ggtaatacag gcatcaatcg ctttcaggtt gtcagtaccg ccaaccgcag caatatagtt
    13441 ggttgccagt tgagtcagac cttcttcagt attgctgttg gcttcttcag taacgatctc
    13501 gtcttcttta tcttcacgac ccggcgtttt caggttgaac atgcggataa ccaaactgaa
    13561 caccacgaag tagatagcga agaagataac gcccatcacc agcagcatcc agacgttctg
    13621 gctggcggcc ggcaggttat acatcaacgc gtagtcgata gcacccgcag agaaagagaa
    13681 gccggcatgg atacccagca gcgttgccac aaacaggctg ataccggtca gcagtgcgtg
    13741 caggaggtac agcagcggcg caaggaacat gaacaggaat tccagcggct cagtcacacc
    13801 ggtcaggaac gcagtaacag caacagaaag cagcatcccg ccaaccatcg gacgacgctc
    13861 tttcggtgct gcgaagtaca tcgccagcgc cgcacccggc agaccgaaca tcatgatcgg
    13921 gaagaagccg gacatgaaca tccccgcggt gccgtcacca gcgtagaaac ggttgatgtc
    13981 gccgtggaaa accgtacccg ccgcgttggt gaattcacca atctggaacc aggcgatggt
    14041 gttcagcacc tgatgcagac cggttgggat cagcagacgg ttgatgaaac caaagatacc
    14101 ggaacccagc gcgcccgcag aaacgatcca ctcgccgcct gcatggatag cgtgctgtac
    14161 cggcggccag acgtaaccaa aaatggccgc cagcaccaga cagaagaagc cggtggcgat
    14221 cggcacaaag cgtttgccgc cgaagaagct caggaagtcc ggcagtttaa tatcggacca
    14281 acggttatag gctgcgccac caaccagacc ggtaatgata cccgccagta cacccatgtt
    14341 aatttctggg ttgatgatca ccatcgcttt ggttaacaca aagtaaccta ccgcacccgc
    14401 cagcgccgcc gcacctgcgc tgtctttcga ccagctggat gccacaccga tggcgaagat
    14461 taatgcgagg ttatcaaaaa ttgcaccgcc cgcctgggca ataaacgcaa cgttaagtaa
    14521 atctggctga ccgaatcgca gcaacagcgc cgccaccggc agcaccgcga tagggagctg
    14581 taacgcccta ccgagtcgct ggaaaaaacc taaaatattc atcttattcc ccctacgaga
    14641 accctatttg gctcgtttaa agccgtattt ttattttgct gcaaatttta ctgccgatgt
    14701 tctgtaatca gattgttaga tcatctgcta cagagtgtgt gaaaatttaa ttcgtatcgc
    14761 aaattaaacg cgtgtctttt gtgagttttg tcaccaaata tcgttatcat cactcccttt
    14821 tactggctaa accagaaaac ttattttatc attcaaaaaa tcaagtcgga ttgacgcctg
    14881 tctgcgcaaa tccaggttac gcttaaagat gtctaatccg ccaacggctt acattttact
    14941 tattgaggtg aataatgaga ctgatccccc tgactaccgc tgaacaggtc ggcaaatggg
    15001 ctgctcgcca tatcgtcaat cgtatcaatg cgttcaaacc aactgccgat cgtccgtttg
    15061 tactgggcct gccgactggc ggcacgccga tgaccaccta taaagcgtta gtcgaaatgc
    15121 ataaagcagg ccaggtcagc tttaagcacg ttgtcacctt caacatggac gaatatgtcg
    15181 gtctgccgaa agagcatccg gaaagctact acagctttat gcaccgtaat ttcttcgatc
    15241 acgttgatat tccagcagaa aacatcaacc ttctcaacgg caatgccccg gatatcgatg
    15301 ccgagtgccg ccagtatgaa gaaaaaatcc gttcttacgg aaaaattcat ctgtttatgg
    15361 gcggtgtagg taacgacggt catattgcat ttaacgaacc ggcgtcttct ctggcttctc
    15421 gtactcgtat caaaaccctg actcatgaca ctcgcgtcgt gaactctcgt ttctttgata
    15481 acgatgtcaa tcaggtgcca aaatatgccc tgactgtcgg tgttggtaca ctgctggatg
    15541 ccgaagaagt gatgattctg gtgctgggta gccagaaagc actggcgctg caggccgccg
    15601 ttgaaggttg cgtgaaccat atgtggacca tcagctgtct gcaactgcat ccgaaagcga
    15661 tcatggtgtg cgatgaacct tccaccatgg agctgaaagt taagacttta agatatttca
    15721 atgaattaga agcagaaaat atcaaaggtc tgtaattgtt atccctgccc tagttccttg
    15781 ctcagggcaa tattttttaa aatcgggggt cagaatgtat gcattaaccc agggccggat
    15841 ctttaccggc cacgaatttc ttgatgacca cgcggttgtt atcgctgatg gcctgattaa
    15901 aagcgtctgt ccggtagcgg aactgccgcc agagatcgaa caacgttcac tgaacggggc
    15961 cattctctcc cccggtttta tcgatgtgca gttaaacggc tgcggcggcg tacagtttaa
    16021 cgacaccgct gaagcggtca gcgtggaaac gctggaaatc atgcagaaag ccaatgagaa
    16081 atcaggctgt actaactatc tgccgacgct tatcaccacc agcgatgagc tgatgaaaca
    16141 gggcgtgcgc gttatgcgcg agtacctggc aaaacatccg aatcaggcgt taggtctgca
    16201 tctggaaggt ccgtggctga atctggtaaa aaaaggcacc cataatccga attttgtgcg
    16261 taagcctgat gccgcgctgg tcgatttcct gtgtgaaaac gccgacgtca ttaccaaagt
    16321 gaccctggca ccggaaatgg ttcctgcgga agtcatcagc aaactggcaa atgccgggat
    16381 tgtggtttct gccggtcact ccaacgcgac gttgaaagaa gcaaaagccg gtttccgcgc
    16441 ggggattacc tttgccaccc atctgtacaa cgcgatgccg tatattaccg gtcgtgaacc
    16501 gggcctggcg ggcgcgatcc tcgacgaagc tgacatttat tgcggtatta tcgctgatgg
    16561 cctgcatgtt gattacgcca acattcgtaa cgctaaacgc cttaaaggcg acaaactgtg
    16621 tctggttacc gacgccaccg cgccagcagg tgccaacatt gaacagttca tttttgcggg
    16681 taaaacaata tactaccgta acggactttg tgtggatgag aacggtacgt taagcggttc
    16741 atccttaacc atgattgaag gcgtgcgtaa tctggtcgaa cattgcggta tcgcactgga
    16801 tgaagtgctg cgtatggcga cgctctatcc ggcgcgtgcg attgacgttg aaaaacgtct
    16861 cggcacgctc gccgcaggta aagtagccaa cctgaccgca ttcacaccta attttaaaat
    16921 caccaagacc atcgttaacg gtaacgaggt cgtaactcaa taagagaaag tatgacacca
    16981 ggcggacaag ctcagatagg taatgttgat ctcgtaaaac agcttaacag cgcggcggtt
    17041 tatcgcctga ttgaccagta cgggccaatc tcgcggattc agattgccga gcaaagccag
    17101 cttgcccccg ccagcgtaac caaaattacg cgtcagctta tcgaacgcgg gctgatcaaa
    17161 gaagttgatc agcaggcctc caccgggggc cgccgcgcta tctccatcgt caccgaaacc
    17221 cgcaatttcc acgcaatcgg cgtacggctt ggtcgtcatg acgccaccat cactctgttt
    17281 gatctcagca gcaaagtgct ggcagaagaa cattacccgc tgccggaacg tacccagcag
    17341 acgctggaac atgccctgct gaatgccatt gctcagttta ttgatagcta ccagcgcaag
    17401 ctgcgcgagc taatcgccat ttcggtgatc ctgccagggc ttgttgaccc ggacagcggc
    17461 aaaattcatt acatgccgca tattcaggta gaaaactggg ggctggtaga agcactgaaa
    17521 gaacgtttta aagtgacctg tttcgttggt cacgatatcc gtagtctggc gctggcggag
    17581 cactacttcg gtgcaagtca ggattgcgaa gactccattc tggtgcgtgt ccatcgcgga
    17641 accggggccg ggattatctc taacgggcgc atttttattg gccgcaacgg taacgtcggt
    17701 gaaattggcc atattcaggt cgaaccgctg ggtgaacgct gccactgcgg caactttggc
    17761 tgcctggaaa ctatcgccgc caacgctgcc attgaacaac gggtgttgaa tctgttaaag
    17821 cagggctacc agagccgtgt gccactggac gactgcacca tcaaaactat ctgcaaagcc
    17881 gcgaacaaag gcgatagcct ggcctcggaa gtgattgagt atgtcggtcg tcatctgggt
    17941 aaaaccatcg ccattgctat caacttattt aatccgcaaa aaattgttat tgctggtgaa
    18001 atcaccgaag ccgataaagt gctgctccct gctattgaaa gctgcattaa tacccaagcg
    18061 ctgaaggcgt ttcgtactaa tctgccggtg gtacgttctg agctggatca ccgctcggca
    18121 atcggcgctt ttgcgctggt aaaacgcgcc atgctcaacg gtattttgct ccagcatttg
    18181 ctggaaaatt aatgtgcttt tatagtggcg cttattgttg tcaatattct gggtagtcca
    18241 tgaccattaa aaatgtaatt tgcgatatcg acgacgtgct gatgcacgat aacgtcgccg
    18301 taccgggtgc agcggaattt ttgcacggga ttatggataa aggcctgccg ctggtgttgc
    18361 tgaccaacta tccttcgcag actgggcaag atctggcgaa ccgctttgcc actgccggtg
    18421 tcgatgtgcc ggatagcgtg ttttatacct ctgcgatggc gaccgccgat ttcctgcgtc
    18481 gccaggaagg caagaaagcg tatgtggtgg gcgaaggcgc actgattcat gaactgtaca
    18541 aagccggttt cactattacc gatgtgaacc ctgatttcgt gattgttggc gaaacacgtt
    18601 cctacaactg ggacatgatg cataaagcag cctatttcgt cgctaacggt gcacgcttta
    18661 tcgccaccaa cccggacacg gtttttatcc cgcttgtggc gcgttgtgtg cagggattga
    18721 gaaaatctcc gggcgcaaac cgttctatgt tggtaagccc agcccgtgga tcatccgcgc
    18781 ggcattaaac aaaatgcagg cgcattcgga agaaacagtg attgtcggcg ataacctgcg
    18841 taccgatatt ctggcgggct tccaggccgg tctggagacg attctggtgc tttctggtgt
    18901 ttcatcgctc gacgatatcg acagtatgcc gttccgccca agctggattt acccgtcagt
    18961 cgctgaaatc gacgttatct gaataccccc cacgtctgcg gacgtggttg gctctgatga
    19021 caaacgccaa actgcctgat gcgctacgct tatcaggcct acgcagctcc tgcaatatat
    19081 tgaattttca tgcttttgta ggccgtataa ggcgttcacg ccgcatccgg catgaacaaa
    19141 tcgcacttta tcagcaattt ggcccccgta aaatataacc attcaataaa acccgctaaa
    19201 aattatcgat tcatcaataa atacatcctc acaccagacc ctttttcacc aatcgacaat
    19261 caccattacg ttttttattt ttgcactgcc aaatagcttg cgcacagtgc tgctttgcgg
    19321 cattttttta aacaagcaaa cacaacaagc aacaaatacc aggttaacgg agaaggttat
    19381 gtgttcaatt tttggcgtat tcgatatcaa aacagacgca gttgagctgc gtaagaaagc
    19441 actcgagctg tcacgcctga tgcgtcatcg tggcccggac tggtccggta tttatgccag
    19501 cgataacgcc attctcgctc acgaacgtct gtcaattgtt gacgttaacg cgggggcgca
    19561 acctctctac aaccaacaaa aaacccacgt actggcggta aacggtgaaa tctacaacca
    19621 ccaggcattg cgcgctgaat atggcgatcg ttatcagttc cagacagggt ctgactgtga
    19681 agtgatcctc gcgctgtatc aggaaaaagg gccggaattt ctcgacgact tgcagggcat
    19741 gtttgccttt gccctgtacg acagcgaaaa agatgcctac ctgattggtc gcgaccatct
    19801 ggggatcatc ccactgtata tggggtatga cgaacacggt cagctgtatg tggcctcaga
    19861 aatgaaatcg ctggtgccag tttgccgcac gattaaagag ttcccggcgg ggagctattt
    19921 gtggagtcag gacggcgaaa tccgttctta ttatcatcgc gactggttcg actttgatgc
    19981 agtgaaagat aacgtgaccg acaaaaacga gctgcgtcag gcactggaag attccgttaa
    20041 aagccatctg atgtctgatg tgccttacgg tgtgctactt tctggtggtc tggattcctc
    20101 aattatttcc gctatcacca agaaatacgc agcccgtcgc gtggaagatc aggaacgctc
    20161 tgaagcctgg tggccgcagt tacactcctt tgctgtaggt ctgccgggtt caccggatct
    20221 taaagcagcc caggaagtgg caaaccatct gggcacggtg catcacgaaa ttcacttcac
    20281 tgtacaggaa ggtctggatg ccatccgcga cgtgatttac cacatcgaaa cttatgatgt
    20341 gaccactatt cgcgcttcaa caccgatgta tttaatgtcg cgtaagatca aggcgatggg
    20401 cattaaaatg gtgctgtccg gtgaaggttc tgatgaagtg tttggcggtt atctttactt
    20461 ccataaagcg cccaacgcta aagaactaca tgaagagacg gtgcgtaaac tgctggccct
    20521 gcatatgtat gactgcgcgc gcgccaacaa agcgatgtca gcctggggcg tggaagcacg
    20581 cgttccgttc ctcgacaaaa aattccttga tgtggcgatg cgcattaacc cgcaggataa
    20641 aatgtgcggt aacggcaaaa tggaaaaaca catcctgcgt gaatgttttg agtcatacct
    20701 gcccgcaagc gtggcctggc ggcagaaaga gcagttctcc gatggcgtcg gttacagttg
    20761 gatcgacacc ctgaaagaag tggcggcgca gcaggtttct gatcagcaac tggaaactgc
    20821 ccgcttccgc ttcccgtaca acacgccgac ctcaaaagaa gcgtatctgt accgcgagat
    20881 ctttgaagaa ctgttcccac ttccgagtgc cgctgagtgc gtgccgggcg gtccttccgt
    20941 cgcgtgttct tcagctaaag cgatcgaatg ggatgaagcg ttcaaaaaaa tggacgatcc
    21001 gtctggtcgt gcggttggcg ttcaccagtc ggcgtataag taagaaatgc ggtactgccc
    21061 cggagaaatc cggggctgtt ttagttattg cgctgcttgt tgattctgat atgcagtaag
    21121 gaaatgttcc gggtttgcca catcactgtt tgacaactca tcactgtagg ccagtatttc
    21181 atagattaat ttcccaccac tattgttgaa ttccgaattt catcctgtac gacaaaagaa
    21241 tggctgtagg gtggggaaaa aacacaaagt aaacaataat tgacgaatat agcgccacgc
    21301 tgttcgcaac ctaaccaaac agtcactttc gagcaatttt ccttgaaaaa gaggttgacg
    21361 ctgcaaggct ctatacgcat aatgcgcccc gcaacgccga taaggtatcg cgaaaaaaag
    21421 atggctacgt agctcagttg gttagagcac atcactcata atgatggggt cacaggttcg
    21481 aatcccgtcg tagccaccat cttttttgcg ggagtggcga aattggtaga cgcaccagat
    21541 ttaggttctg gcgccgcaag gtgtgcgagt tcaagtctcg cctcccgcac cattcaccag
    21601 aaagcgttgt acggatgggg tatcgccaag cggtaaggca ccggtttttg ataccggcat
    21661 tccctggttc gaatccaggt accccagcca tcttcttcga gtaagcggtt caccgcccgg
    21721 ttattggggt atcgccaagc ggtaaggcac cggtttttga taccggcatt ccctggttcg
    21781 aatccaggta ccccagccat cgaagaaata atctggctac gtagctcagt tggttagagc
    21841 acatcactca taatgatggg gtcacaggtt cgaatcccgt cgtagccacc aaattctgaa
    21901 tgtgtcgaat acgttcggca aattcaaaaa ccaatttgtt ggggtatcgc caagcggtaa
    21961 ggcaccggat tctgattccg gcattccgag gttcgaatcc tcgtacccca gccacattaa
    22021 aaaagctcgc ttcggcgagc tttttgcttt tctgcgttca ttcaatgtcg aatgcgatgt
    22081 tgacacgtct tatccttcaa tgtcggatgc gacgctgccg cgtcttatcc gacctacggt
    22141 tggcacgcat ccggcaatgt tgtaaggcta caaccctaac gcatatttca gcgcctgacg
    22201 tttcaacacg ccagcacgct ctgccgccat taacccgaga ttacgcacaa aacgcagcgg
    22261 cggcagatta ttactgaatc cggcataaaa cagatccata ccactttgca taatgaagtt
    22321 atccgccatg cgccgcatct ggtaacgctt gaggacagga taactggccc acgcttcacc
    22381 gtagctgcgg gcattcacca gaacatcaat cagggcatcg acatcacgat aaccaagatt
    22441 caccccctgc cccgccagcg gatggatggt atgcgcggca tcgcccacca gcgcaagccc
    22501 cggctgcaca tactgcaacg catgtcggcg cgtcagcgga aacgcaccag cggcaagcgg
    22561 tgtaacgtaa cccagacgcg acgggaaatg cttcgcgatt tccgcctgga gttgcgccat
    22621 attcatattc tgcaactggc gaatacgcgc cggagagtca taccacacca gcgatgccca
    22681 gttatcaaac aacggcagaa acgcacgcgg tccgtccggg gtaaattgct gccaggtgct
    22741 gtcgccggga tcgttctcac actgtacgct aatcaacatg cacgactgcg catactgcca
    22801 ggcatgaacg ccaattcccg ccatctgccg cacctgagaa tttgcgccgt cggcaccaat
    22861 caccagtttc gcgcggatcg tttcaccgcc cttcagttcc agctcctgaa gatcattatc
    22921 tcgatgtaat gcaatcagcg aggttggcac acgtaacgtt actttcggat gcgcttccag
    22981 cgcctgccac aacgcctgtt gcaggactgt gttttccacc atgtagccaa gcaacggcaa
    23041 ctttaattcg gctgcgtcaa ataccacatg tgccgtttcc cactcccagg tttccagtct
    23101 gcggtaagga tggcaacgca tagcctgtac tgcatcccag acccctaccc ctttaagcaa
    23161 tgataccgaa gccgcgctga tcgccgagat ccgcacgtcc ggttggctgt cagcgacaaa
    23221 cggtgcaggt tctgcgtgct cgatcaccgt taccgaaaat ccgtgctgtg ccagccccag
    23281 cgccagtgcg ccgccgacca ttcctccgcc gacaatggca atttccgttg gttgatttgt
    23341 catggtcaat catcctgtta gcaatatgct ttaagtttac aggattttta cccccaaggg
    23401 ctgataacgc tcatactggt cacaacggca gcaaagcatt acactatgcg ccctgcattc
    23461 ctggctacta tttcgcaaga gcaagtcgat gaccaaaaaa ctccatatta aaacctgggg
    23521 ctgtcagatg aacgagtacg attcatcgaa gatggccgat ctgctggatg ccacccacgg
    23581 ctatcaactg accgacgtgg cggaagaagc ggatgtgcta ctgctgaaca cctgctcaat
    23641 ccgcgagaag gctcaggaaa aagtcttcca tcagttgggt cgctggaaac tgttaaaaga
    23701 gaagaatcca gacctgatta tcggcgtcgg tggctgcgtg gcatcgcaag aaggcgagca
    23761 cattcgccag cgcgcccact atgtcgatat tatttttggg ccgcaaacgc tgcaccgtct
    23821 gccggagatg atcaactccg tgcgcggcga ccgcagcccg gttgtagata tcagcttccc
    23881 ggaaatcgag aagtttgacc gtctgccgga accgcgcgcc gaagggccga ccgcgtttgt
    23941 ctccatcatg gaaggctgca ataaatattg cacctactgc gtggtgcctt acacccgtgg
    24001 tgaagaggta agccgtccgt ctgacgatat tctgtttgag attgcccagc ttgcggctca
    24061 gggcgtgcgt gaagtcaacc tgctcggtca gaatgtgaat gcctggcgtg gtgagaacta
    24121 cgacggcacc accggatcgt ttgccgatct gttgcgtctg gttgctgcga tcgacgggat
    24181 cgatcgtatt cgctttacca ccagccatcc gatcgaattc accgacgata tcatcgaagt
    24241 gtaccgcgac acgcctgagc tggtgagctt cctgcatctg ccggtacaga gcggttccga
    24301 tcgcattctg aacctgatgg gacgtaccca tacggcgctg gagtacaaag cgatcatccg
    24361 taaactgcgt gcggcgcgtc cggatattca gatcagttct gacttcattg ttggcttccc
    24421 tggcgaaacc accgaagact tcgagaaaac gatgaagctg attgccgacg tcaatttcga
    24481 catgagctac agctttatct tctctgcacg tccaggaacg cctgccgccg atatggttga
    24541 tgatgttccg gaagaagaga agaagcagcg tctgtatatt ctgcaagaac gtatcaatca
    24601 gcaagcgatg gcatggagcc gtcggatgct cggcaccacc cagcgtattc tggtagaagg
    24661 gacttcgcgt aagagcatca tggagctttc cgggcgtacc gaaaataacc gcgtggtcaa
    24721 cttcgagggc acaccggata tgatcggtaa attcgtcgat gtagaaatta ccgacgtcta
    24781 cccgaactct ctgcgcggta aagtggtgcg aactgaagat gaaatgggtc tgcgcatggc
    24841 agaaacaccg gagtcggtga ttgcccgcac ccgcaaagaa aacgaccttg gcgtgggtta
    24901 ttatcagccg taattctcag gcctgccgtt ccggtgggcc ttgttttttc tctctttatc
    24961 cccatcttta tcgcaatgct tgcgcctttt tcctgtggcg tgaataattt ccttataggc
    25021 cggtgaatgt tcagcttccg gcggcataca gataagagga acggtttgaa catagacact
    25081 cgcgaaatca ccctggagcc agcagacaat gcgcgtctgt tgagcctgtg cggcccgttt
    25141 gatgacaaca tcaagcagct cgaacgccgt ctcggcatcg agatcaatcg ccgcgataac
    25201 cactttaaac tgaccggccg tccgatttgc gtcaccgctg cggcagacat tctgcgtagc
    25261 ctgtatgtcg atactgcccc gatgcgcggt cagattcagg atatcgaacc ggaacagatc
    25321 caccttgcga ttaaagaagc gcgggtactg gagcaaagcg cggagagcgt gccggagtac
    25381 ggcaaagcgg tcaatatcaa aactaaacgc ggcgtaatta agccgcgcac gccaaaccag
    25441 gcgcagtaca tcgccaatat tctcgaccat gacattacct tcggcgttgg cccggcgggt
    25501 acgggtaaaa cctacctggc agtggctgcg gcagttgatg ccctggagcg tcaggaaatt
    25561 cgccgtattc tgctgactcg tccggcggtc gaagccggtg agaaactggg cttcctgcct
    25621 ggcgatttaa gccagaaagt agacccgtat ctgcgcccac tgtacgacgc gctgtttgaa
    25681 atgctgggct ttgagaaagt cgagaaactg attgagcgca acgttattga agtcgcgccg
    25741 ctggcctata tgcgtggtcg tacgctgaac gacgcgttta tcattctcga tgagagccag
    25801 aacactacca tcgaacagat gaagatgttc ctgacccgta tcggttttaa ctcaaaagcg
    25861 gttatcaccg gcgacgtcac acagatcgac ctgccgcgta atactaaatc aggcttacgt
    25921 catgccattg aagtgctggc cgatgtcgaa gagatcagct ttaacttctt ccacagcgaa
    25981 gacgtggttc gtcacccggt ggtggcgcgt atcgttaacg cttatgaagc cagggaagaa
    26041 gcagaacaaa aacgtaaagc ggcgctggca gcagaacgca agcgcgaaga acaggaacaa
    26101 aaatgagtca ggtgatcctc gatttacaac tggcatgtga agataattcc gggttaccgg
    26161 aagagagcca gtttcaaaca tggctgaatg cggtgatccc gcagtttcag gaagagtcgg
    26221 aagtgactat tcgcgtggtc gataccgccg aaagccacag tctgaatctg acctaccgcg
    26281 gtaaggataa gccgaccaac gtgctctcct tcccgtttga agtgccgcct ggcatggaga
    26341 tgtcgctact gggcgatctg gttatctgcc gtcaggtggt tgagaaggaa gctcaggagc
    26401 aaggcaaacc actggaggcg cactgggcgc atatggtggt gcacggcagt ctgcatttgt
    26461 taggttacga tcacattgaa gatgacgaag cagaagaaat ggaagccctc gaaacagaga
    26521 ttatgcttgc tctgggctat gaggatccgt acattgccga gaaagaataa tttgccaggc
    26581 atttgactgg cagacgtccc cgccgttggt gattaacgac ggcgttttga ttaactaaca
    26641 tgagaaccca tacgacgcca tgagcgacga caattcacac agtagtgaca cgataagcaa
    26701 caagaaggga tttttctccc tgttactcag ccaacttttc cacggtgaac cgaaaaaccg
    26761 tgacgaactg ctggcgctga tccgtgattc cgggcagaac gaccttatcg acgaagatac
    26821 gcgcgatatg ctcgaagggg tgatggacat cgcagaccaa cgcgttcgcg acatcatgat
    26881 cccccgctcc cagatgatta ccctgaaacg caaccagacg ctggacgaat gccttgatgt
    26941 catcatcgag tccgcccact cacgtttccc ggtgattagc gaagacaaag atcacattga
    27001 agggattctg atggcgaaag acttgctgcc gtttatgcgc agcgatgctg aagccttcgg
    27061 catggacaaa gtgttacgtc aggcggttgt cgttcctgaa agtaagcgcg tagaccggat
    27121 gctgaaagag tttcgctctc agcgttacca catggcgatc gttattgacg aattcggtgg
    27181 ggtttccggt ctggtgacca ttgaagacat cctggaactg attgttggtg agattgaaga
    27241 cgagtatgac gaagaagatg atatcgactt ccgtcagctg agtcgtcata cctggaccgt
    27301 gcgcgcactg gcttccattg aagacttcaa cgaagcgttc ggcacccact ttagcgatga
    27361 agaagtcgac actatcggtg gtctggtgat gcaggcgttt gggcatcttc cggcgcgtgg
    27421 cgaaactatc gacatcgacg gttaccagtt caaagtggcg atggccgata gtcggcgtat
    27481 tattcaggtt catgtcaaaa tcccggatga ctcaccccag ccaaagctgg atgaataaaa
    27541 ccgaaactgg atagataact acatggcttt tgcctcatta attgaacgcc agcgcattcg
    27601 cctgctgctg gcgttattat tcggtgcctg cggaacgctg gccttctctc cttacgacgt
    27661 ctggcctgcg gcgattattt cgctgatggg gcttcaggcg ttgaccttta accgtcgccc
    27721 actccagtct gccgctattg gcttttgctg gggatttggc ctctttggca gcggtattaa
    27781 ctgggtctat gtcagcatcg cgacctttgg cgggatgcct ggcccggtta acatcttcct
    27841 ggtggtgctg ctggcggcgt atttgtcgct gtataccgga ctgtttgctg gcgtgctgtc
    27901 gcgtctgtgg ccgaaaacca cctggctgcg cgtggcgatt gccgcccctg ctctctggca
    27961 agtgaccgag tttctgcgcg gttgggtgct gactggcttc ccgtggttac agttcggcta
    28021 tagccagatt gatggcccgt taaaagggct ggcaccgcta atgggcgtgg aagccattaa
    28081 cttcctgctg atgatggtta gtggcctgct ggcactggcg ttggtcaaac gcaactggcg
    28141 tccgctggtg gtggccgtcg tgctgtttgc cctacccttc ccgctgcgtt acatccaatg
    28201 gtttaccccg caaccggaga aaaccattca ggtttcgatg gttcagggcg atattccgca
    28261 atcgctgaaa tgggacgaag gccagcttct taatacgcta aagatttact acaacgcaac
    28321 ggcaccgctg atgggcaaat catcgttgat tatctggccg gagtcggcga taaccgatct
    28381 ggaaattaat cagcaaccgt tcctcaaagc actggacggt gagttgcgtg ataaaggtag
    28441 ctcgctggta accgggattg tcgacgcgcg tctcaataag cagaaccgct acgataccta
    28501 caacaccatc atcacgctgg gtaaaggtgc accgtacagc tacgaatcag ccgatcgcta
    28561 taacaaaaac catctggtgc cgtttggcga gtttgtcccg ctggagtcga ttctgcgtcc
    28621 gttagcaccg ttctttgatc tgccgatgtc gtcgttcagc cgtgggccat atatccagcc
    28681 gccgctgtcg gcaaatggta ttgagcttac tgcggctatt tgctacgaga tcattctcgg
    28741 cgagcaagtg cgcgataact tccgcccgga taccgactac ctgctgacta tctccaacga
    28801 tgcgtggttt ggtaaatcta ttggtccatg gcaacacttc cagatggcgc gaatgcgtgc
    28861 gctggagctg gcgcgcccac tgttgcgcag caccaacaac ggcattacgg cggtgattgg
    28921 cccgcagggt gagattcagg cgatgatccc gcagttcacc cgcgaggtgt taaccactaa
    28981 cgtgacgccg accaccggac tcacaccata cgcacgtacc ggcaactggc cgctgtgggt
    29041 gctgacggca ctgtttggtt ttgctgctgt gttgatgagt ctgcgtcagc gacgtaaata
    29101 atcccttctt atcgtgccgg aatacatccc atatttcggc acgctcaccg gatgcggcat
    29161 cagtgcatta tccggataac aatattctcc tatccaactt tggcacatct attgctttgt
    29221 tatacaaggc aaagcttgaa ccagcatcag tacaacccgg ttgcaccaca gcaatccacc
    29281 ggtagcacca aaagagtgca ttttaagaat tttgcttcag aatggtgcgg tgcacctcgc
    29341 aaaaacaaac aataaaacat caatatcttt acatttagcc aaactaaatg ttacctaaca
    29401 ggcacaacac tgcacaataa agttgcagac gataacaaca caaacactca caacgggtat
    29461 ccatgcgttc ttaacgcaga agataaagga gttggatatg caattacgta aacctgccac
    29521 agcaatcctc gccctggcgc tttccgcagg actggcacag gcagatgacg ccgccccggc
    29581 agcaggcagt acgctggaca aaatcgccaa aaacggtgtg attgtcgtcg gtcaccgtga
    29641 atcttcagta cctttctctt attacgacaa tcagcaaaaa gtggtgggtt actcgcagga
    29701 ttactccaac gccattgttg aagcagtgaa aaagaaactc aacaaaccgg acttgcaggt
    29761 aaaactgatt ccgattacct cacaaaccgt attccactgc tgcaaaacgg cactttcgat
    29821 tttgaatgtg gttctaccac caacaacgtc gaacgccaaa aacaggcggc tttctctgac
    29881 actattttcg tggtcggtac gcgcctgttg accaaaaagg gcggcgatat caaagatttt
    29941 gccgacctga aaggcaaagc cgtagtcgtc acttccggca ctacctctga agttttgctc
    30001 aacaaactga atgaagagca aaaaatgaat atgcgcatca tcagcgccaa agatcacggt
    30061 gactctttcc gcaccctgga aagcggtcgt gccgttgcct ttatgatgga tgacgctctg
    30121 ctggccggtg aacgtgcgaa agcgaagaaa ccagacaact gggatatcgt cggcaagccg
    30181 cagtctcagg aagcctacgg ttgcatgttg cgtaaagacg atccgcagtt caaaaagctg
    30241 atggatgaca ctatcgctca ggtgcagacc tccggtgaag cggaaaaatg gtttgataaa
    30301 tggttcaaaa atccaattcc gccgaaaaac ctgaacatga atttcgaact gtcagacgaa
    30361 atgaaagcac tgttcaaaga accgaatgac aaggcactga actaattaca agaaccaggg
    30421 gcggaaattc cagccctctc gattgttacg tagcacggac agactatacg cctgatggtc
    30481 gttccccatc gggcctgaaa accgcaatac gctgggtaac aatcttcgag ggtagcagtt
    30541 aacgctgcta ccctcttttt tctggagtag atttatgtct atagactgga actggggtat
    30601 ttttttacaa caagccccgt tcggcaatac cacctatctt ggttgggtct ggagtggttt
    30661 tcaggtgacg atcgctttgt cgatctgcgc ctggattatc gctttcctcg tcggttcatt
    30721 tttcggcatt ttacgtaccg ttccaaaccg ttttctctcc ggtctgggta cgttgtatgt
    30781 cgaactgttc cgcaacgtgc cgttgattgt gcaattcttt acctggtatc tggtgatccc
    30841 ggagctgctg ccggagaaaa tcggcatgtg gtttaaggcc gagctggatc ccaatattca
    30901 gtttttcctt tcatccatgc tctgcctggg gctgtttacc gcctcccgtg tttgcgaaca
    30961 ggtacgcgcc gctattcagt cgctaccgcg tgggcagaag aatgccgcgc tggcgatggg
    31021 cctgacgcta ccgcagactt atcgatacgt actgctgcct aatgcttatc gcgttatcgt
    31081 cccgccgatg acttccgaga tgatgaacct ggtgaaaaac tccgccatcg cctcgaccat
    31141 cggtctggtg gatatggcgg cgcaggcggg taaattgctc gactactcgg ctcacgcctg
    31201 ggagtcgttt accgccatca cgcttacgta tgtgttgatt aacgctttta tcatgctggt
    31261 gatgacgctg gttgaacgta aagttcgcct gcccggcaac atggggggca aataatgtac
    31321 gagtttgact ggagttccat tgttccttcc ctgccatatc tgctcgacgg gctggtgatc
    31381 accctgaaaa tcaccgtcac ggcagtcgtg attggtattt tgtggggtac catgctggcg
    31441 gtgatgcgct tgtccagctt cgcgcctgtt tcgtggtttg ccaaagccta cgttaacgta
    31501 ttccgctcga ttcctttagt catggttttg ctgtggtttt acttgattgt gccgggattt
    31561 ctgcaaaatg tgctgggatt atcgccaaaa aatgatattc gtttaatttc ggcgatggtg
    31621 gcgttttcga tgtttgaagc ggcctactat tcagagatta tccgtgccgg tattcaaagt
    31681 atttcccgtg gtcagtcgag cgccgcgctg gcgttgggga tgactcactg gcagtcgatg
    31741 aaactgatta ttctgccgca ggctttccgc gcgatggtgc cgctgctgct cactcagggc
    31801 atcgtactgt tccaggatac ctcgctggtg tatgtgttat ctctggccga tttcttccgt
    31861 accgcctcaa ccattggtga gcgtgatggt acgcaggtcg agatgatcct gtttgccgga
    31921 tttgtttatt tcgttattag ccttagcgcg tcgttgttgg tcagctactt gaaaagaagg
    31981 acagcataat gattaccctg aaaaatgttt caaaatggta tggtcacttt caggtgctga
    32041 ccgactgctc aaccgaagtg aaaaaaggcg aagtggtggt ggtttgcggc ccgtctggtt
    32101 ccggcaaatc aacgctgatt aaaaccgtca acggcctcga accagtgcag caaggtgaaa
    32161 tcaccgtcga tggtatcgtg gttaacgaca agaaaaccga tctggcaaag ctgcgctccc
    32221 gcgtcgggat ggtattccag catttcgagc tgttccctca tctgtcgatt atcgaaaacc
    32281 tgaccctggc gcaggtgaaa gtgcttaaac gcgataaagc gcccgctcgt gaaaaagcat
    32341 tgaaattgct ggagcgcgtg ggactttccg cacatgccaa taaatttccg gcgcagcttt
    32401 ccggcggtca gcagcagcgt gtggcaatcg ctcgcgcatt gtgtatggct cctattgcga
    32461 tgctgttcga cgaaccgaca tcggcgctgg atccggagat gatcaacgaa gtgctggacg
    32521 tgatggtgga actggcgaac gaaggaatga ccatgatggt ggtgacccac gaaatgggct
    32581 ttgcccgcaa agtggcgaat cgggtgatct ttatggacga gggtaaaatt gtcgaagact
    32641 cgccgaaaga cgctttcttc gatgatccga aatcggaccg cgcaaaagac ttcctcgcga
    32701 aaatcctgca ttaatcactc tggcgcgcat acccaattgc gcgccatctc acgctttatc
    32761 gtttcgcccc tcgtatctgt tacatcctgg cgttaccctt gtcgcaaaga agcacacaac
    32821 aaggagcaac aatggcactg ccaattctgt tagattgcga cccaggtcat gatgacgcta
    32881 tcgcaatagt tctcgccctc gcctcaccag agcttgatgt caaagcaatt acgtcttccg
    32941 ccggaaacca gacaccagaa aaaaccttac gcaatgttct gcgtatgctg accttgctta
    33001 atcgcaccga tattccggtg gcaggcggcg cggtaaaacc gttaatgcgt gagttgatta
    33061 tcgcggacaa tgtgcacggc gaaagcggtc tcgacggccc ggcattaccg gaaccgacgt
    33121 tcgcaccgca aaactgtacg gcggtggagc tgatggcgaa aacgctgcgc gaaagtgcgg
    33181 aacctgtcac gattgtttct accggaccgc aaactaacgt tgccttgctg ctcaatagcc
    33241 atccggaact gcatagcaaa attgcccgta tcgtgattat gggcggcgca atggggcttg
    33301 gtaactggac gcctgcggct gaatttaata tttacgttga cccggaagcg gcagaaattg
    33361 tcttccagtc agggatcccg gtggtgatgg ccggtctgga tgttactcat aaagcgcaaa
    33421 tccacgttga agacaccgag cgtttccgcg cgattggcaa ccctgtttca accattgttg
    33481 ccgaactgct ggatttcttc ctcgaatatc ataaagacga aaaatggggc tttgtcggcg
    33541 caccgctgca tgacccatgc accatcgcct ggctgttgaa acccgagttg tttaccactg
    33601 ttgagcgctg ggttggcgtg gaaacacagg ggaaatatac cctgggtatg acggttgttg
    33661 attattatta tctgactggc aataaaccga atgccactgt aattgtcgat gttgatcgtc
    33721 agggctttgt tgatttactg gctgatcgtc tgaaatttta cgcttaacac tcgtcattga
    33781 ttaagatcac cacgcgttac ctgggtggtc ttaatctatt tatctctttt tctttcaaga
    33841 tataaatgca atggataatg cagaactcgc cataggtatc gatctcggca ctaccaatag
    33901 tttaattgcc gtctggaaag acggtgccgc gcaattaatt ccaaataagt tcggtgaata
    33961 tttaacacca tccataatta gcatggatga aaataatcat attttagtcg gaaaaccggc
    34021 tgtatcacgg cgtacttcgc atccggataa aacggcagcg ttatttaaac gtgcaatggg
    34081 cagtaatacc aactggcggt taggcagcga cacatttaac gcgccagaac tgtcctcttt
    34141 ggtattacgc tcattaaaag aagatgccga agaatttctg caacgtccga ttaaagatgt
    34201 ggtgatctcc gttccggctt atttcagcga taaacaacgc aagcataccc gtttagcagc
    34261 ggacttagcc gggttaaatg cggtacgctt aattaatgaa cccacagcag ctgcgatggc
    34321 gtatggcctg catacccaac aaaatacccg ttcgctggtg tttgatctcg gtggcggcac
    34381 gtttgacgtt acggtgcttg agtacgccac gccggtgatt gaagttcacg cctccgctgg
    34441 cgacaacttc ctcggtggcg aagattttac ccatatgttg gtcgatgacg ttttaaagcg
    34501 cgcggatgtc acaaggacca cgcttaacga gagtgaactg gcagccttgt acgcctgtgt
    34561 ggaagcggca aaatgtagca atcaatcgcc aatgcacatt cgctggcagt accaggatga
    34621 aacgcgggaa tgcgaatttt acgagaacga actggaagat ttgtggttgc cgctgctcaa
    34681 tcgcttgcga gtgccgattg aacaggcgtt gcgcgatgcg cgtctgaagc cgagtcaaat
    34741 cgacagtctg gtgctggttg gcggcgcgtc acaaatgccg ctggtgcagc gaatcgccgt
    34801 gcgtctgttt ggcaaattac cgtatcaaag ttacgatccg agcaccattg tcgcgctggg
    34861 cgcagcaatc caggccgcct gccgcttacg cagtgaagat attgaagaag tgatcctcac
    34921 tgatatttgc ccttactcgt tgggcgttga ggttaaccgc cagggcgttt ccggcatttt
    34981 ctcgccgatt attgaacgaa acaccactgt gcccgtgtcg cgtgtagaaa cttattcaac
    35041 catgcacccg gaacaggatt caattacggt taacgtctat cagggagaaa accacaaagt
    35101 taaaaacaac attctggtgg aatccttcga tgtgccgttg aagaaaaccg gggcttatca
    35161 gtcgattgat attcgcttta gttatgatat caacgggttg cttgaagttg acgtgcttct
    35221 ggaagacggc agcgttaagt ccagagtgat taaccacagc ccggtaacat tgagcgcgca
    35281 gcagattgaa gagagtcgga cgcggttatc cgcattgaaa atttatccgc gcgatatgct
    35341 catcaatcgc acctttaaag ccaaactgga agagttgtgg gcgcgggcgc tgggtgacga
    35401 gcgagaagag attggccggg tgatcaccga ttttgatgcg gcgctgcagt caaacgatat
    35461 ggcccgcgtc gatgaggtca ggcggcgggc gagcgtttat ttagccattg agacctcata
    35521 atcacggctg ccggatgcgg cgtaaatgct gcatccggca tcggcgcaaa aaacacatcg
    35581 tcaactaaac aacatcgcga ttttgccgag agcataacct ataaccagca ccgttaaact
    35641 gattttgata atgctggtaa tgctctgtgg tttttcaccc tcatcatttt tttcttcatg
    35701 gttttcaggt gttggctcat cgacatcttc atcctccggc tcgagtttta ctttcgtcaa
    35761 cagcatccgg ttgataatgg cctctaatgg atgagcatct ccgccttcca gccttgtatt
    35821 aagacgggcc agcatctctt gtttcggaaa cgcttcaagt actacctgtt ccgctacccg
    35881 atacaaatcc agtaacggtg aatcttcttc tgtctcgcta ccgactctcg atttccacca
    35941 cgcccatgct ttgcgtttga agaataacga ctcaataaac gattgaccta acggcgataa
    36001 ttttccgcta tgtaaaattt ccgcccataa cagccgcgtc cgggcgctct caccaggaac
    36061 atatttcagc ggttgacctt gcgcatctac catgctccgc gcttcgataa ccatcactaa
    36121 taacgcgaaa taatccgggc aatgacgctt gctccactgc aacaacaaat tatcagcttg
    36181 tgtagatggg taactttccc agtacgcgcc gagatcagca agcaggcttt ccccctcgcc
    36241 gcagtacaaa cgttgtgcac actggtaata ccgggcatct tcgctttctg gttcttccgc
    36301 ttgccactgt ttggctacag gaatgagttc caccatacct gtttgcaccg agctgtacca
    36361 gcgcaacagt ttgcgatgta actttgcatc atcaggaatg aaccacggtc cttccatcgc
    36421 caaaaacgca gtgacatagt caggatggta acggcagcaa cgttccagcg cgaagtaata
    36481 ttcaacagtc tggttctgta acgcgacggg atattccaga aggctaagga aatcgaaaca
    36541 atcaccagcc tgaatggcct caagaaaagc ctccctttct tcctcatcca ttccttcgct
    36601 gtcattaaac gaatgccagt tcaaacgctc tgccaacaaa gaagcacaat ttaatgacag
    36661 atatcgcgct tctatagcga ttgcacacag cggccagcgt aattgatcga catcctccat
    36721 gttccaggta tttaattgct gaataaattt ctgccaagcg gagggctgaa aacgatcact
    36781 ttctgaatcc agtaatgtcc tgaatgcacg tagtatttca tgttcagcgg tggcatcttt
    36841 ttcttcatca tctgcttcct cgacaggatt taccgccagt cgcagcgcct cttcataggc
    36901 ctggcgtaat gctttaaacc cctgcggatc ggtttcggga tgacacaacg gtaagcgagc
    36961 aagataagcc tggcggataa tgtctatttg cgtcgtgctt tcaatttcga ggatttgcca
    37021 acatgttttc ataggtcgct cacatcctta agcgttaccg ggcattcagg ctggacagtt
    37081 aatggctgcc agggaagcgt ggcatagaaa cggtcgcttc ccgctgccag ctcttcgaac
    37141 atccgactcc cggcgctatc gtatttgtac agggttagcg cctcgcgtag cgaagcgttg
    37201 tcttcgcact gagaagattg ccagtagaga cgccctagtg aataggcaga aaaatagtgc
    37261 atccagctgt gatagtaatg gtgagctcgt gcatagacgc gcgattgcag ccataaactc
    37321 tcttcttccg tcagccagtt gttgagcaca ccggcccgtg acagaaaccc cattcgaaca
    37381 taatcccagg ctttgattcc cccttctcca cagcacatag cggtttgggc aacgaattgg
    37441 gtataaatca accctctctc cgagccacca gcaattaatg ctttccactc ttcaggggaa
    37501 gagcgttgcc atgtcagata taagccagct aaatcattgg catgaccatc atcagtcatc
    37561 tgataaatag tctgaattaa atcatcacga ttatttattc cccaggatga tgagagatcg
    37621 aattctttta tcccggtata catttttggc gagtcatgcg cgggtttgca actctgatta
    37681 agtatttcca tgggcgcaga aagcgcaaaa agaagatatt gttcttcctt attcattttt
    37741 taataatccc ttaagtgcat taactgacta cttttttatt attgatgagt ataaacttga
    37801 cgctttcaaa ataaaaagaa aatcgaagca ttcacacatg aataaaaaat taatgtatat
    37861 attcgca
//
LOCUS       DAJTYG010000029        37625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTYG010000029 DAJTYG010000000
VERSION     DAJTYG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.029"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..174)
                     /locus_tag="OO568_001597"
     CDS             complement(<1..174)
                     /locus_tag="OO568_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR6640987.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQYR"
     gene            complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="OO568_001598"
     CDS             complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="OO568_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6640988.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     YYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1228..1524)
                     /gene="fis"
                     /locus_tag="OO568_001599"
     CDS             complement(1228..1524)
                     /gene="fis"
                     /locus_tag="OO568_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6640989.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="OO568_001600"
     CDS             complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="OO568_001600"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6640990.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="OO568_001601"
     CDS             complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="OO568_001601"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6640991.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKTEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3737..5188)
                     /gene="panF"
                     /locus_tag="OO568_001602"
     CDS             complement(3737..5188)
                     /gene="panF"
                     /locus_tag="OO568_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6640992.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5178..5420)
                     /locus_tag="OO568_001603"
     CDS             complement(5178..5420)
                     /locus_tag="OO568_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6640993.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5529..6878)
                     /gene="accC"
                     /locus_tag="OO568_001604"
     CDS             complement(5529..6878)
                     /gene="accC"
                     /locus_tag="OO568_001604"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6640994.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVQSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNTIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6889..7359)
                     /gene="accB"
                     /locus_tag="OO568_001605"
                     /gene_synonym="fabE"
     CDS             complement(6889..7359)
                     /gene="accB"
                     /locus_tag="OO568_001605"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6640995.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8337..9311)
                     /locus_tag="OO568_001606"
     CDS             complement(8337..9311)
                     /locus_tag="OO568_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6640996.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPSTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9463..11403
                     /gene="csrD"
                     /locus_tag="OO568_001607"
     CDS             9463..11403
                     /gene="csrD"
                     /locus_tag="OO568_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6640997.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVIEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11708..12751
                     /gene="mreB"
                     /locus_tag="OO568_001608"
     CDS             11708..12751
                     /gene="mreB"
                     /locus_tag="OO568_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6640998.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12817..13920
                     /gene="mreC"
                     /locus_tag="OO568_001609"
     CDS             12817..13920
                     /gene="mreC"
                     /locus_tag="OO568_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6640999.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13920..14408
                     /gene="mreD"
                     /locus_tag="OO568_001610"
     CDS             13920..14408
                     /gene="mreD"
                     /locus_tag="OO568_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6641000.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14417..15010
                     /gene="yhdE"
                     /locus_tag="OO568_001611"
     CDS             14417..15010
                     /gene="yhdE"
                     /locus_tag="OO568_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6641001.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15000..16469
                     /gene="rng"
                     /locus_tag="OO568_001612"
     CDS             15000..16469
                     /gene="rng"
                     /locus_tag="OO568_001612"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6641002.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16537..20337
                     /gene="yhdP"
                     /locus_tag="OO568_001613"
     CDS             16537..20337
                     /gene="yhdP"
                     /locus_tag="OO568_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6641003.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QSAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLIEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKAR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20493..21938
                     /gene="tldD"
                     /locus_tag="OO568_001614"
     CDS             20493..21938
                     /gene="tldD"
                     /locus_tag="OO568_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6641004.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="OO568_001615"
     CDS             complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="OO568_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR6641005.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23178..23381
                     /locus_tag="OO568_001616"
     CDS             23178..23381
                     /locus_tag="OO568_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6641006.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23389..24321
                     /gene="aaeA"
                     /locus_tag="OO568_001617"
     CDS             23389..24321
                     /gene="aaeA"
                     /locus_tag="OO568_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6641007.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24327..26294
                     /gene="aaeB"
                     /locus_tag="OO568_001618"
     CDS             24327..26294
                     /gene="aaeB"
                     /locus_tag="OO568_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6641008.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26386..26658
                     /locus_tag="OO568_001619"
     CDS             26386..26658
                     /locus_tag="OO568_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6641009.1"
                     /translation="MNIYAFDFNEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO568_001620"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO568_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6641010.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATPYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO568_001621"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO568_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6641011.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="OO568_001622"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="OO568_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6641012.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO568_001623"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO568_001623"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6641013.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31772)
                     /gene="degQ"
                     /locus_tag="OO568_001624"
     CDS             complement(30405..31772)
                     /gene="degQ"
                     /locus_tag="OO568_001624"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR6641014.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31926..32324)
                     /locus_tag="OO568_001625"
     CDS             complement(31926..32324)
                     /locus_tag="OO568_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6641015.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32518..33645
                     /gene="zapE"
                     /locus_tag="OO568_001626"
     CDS             32518..33645
                     /gene="zapE"
                     /locus_tag="OO568_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6641016.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAEIDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33864..34292
                     /gene="rplM"
                     /locus_tag="OO568_001627"
     CDS             33864..34292
                     /gene="rplM"
                     /locus_tag="OO568_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6641017.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34308..34700
                     /gene="rpsI"
                     /locus_tag="OO568_001628"
     CDS             34308..34700
                     /gene="rpsI"
                     /locus_tag="OO568_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6641018.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35095..35733
                     /gene="sspA"
                     /locus_tag="OO568_001629"
     CDS             35095..35733
                     /gene="sspA"
                     /locus_tag="OO568_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6641019.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLINTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35739..36236
                     /gene="sspB"
                     /locus_tag="OO568_001630"
     CDS             35739..36236
                     /gene="sspB"
                     /locus_tag="OO568_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6641020.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36279..37625)
                     /gene="dcuC"
                     /locus_tag="OO568_001631"
                     /pseudo
     CDS             complement(36279..37625)
                     /gene="dcuC"
                     /locus_tag="OO568_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
BASE COUNT         9110 a         9411 c         9904 g         9200 t
ORIGIN      
        1 tctgtactga aaattcagtt cgtcacagtt aaccagtttt agtggattaa ctgagttcat
       61 attacggcat ttgtctgttt ctgttgtcag cagttcaatc cactgcatca gtaaagtatc
      121 aaacagaaaa acgaagacag cgaaaacgac gagccaccaa tacttgcgaa tcattacctg
      181 agcctgtgca tgtttaacca agtctggcag gaaatataca cgaaatgaat aatctgaaaa
      241 ccaggcttac acttccttta ctttgcaatg agatcggggt caacttcgct taaccggctt
      301 cgttttgaca ggttgccggt ctttcttttt ttcactttcg ccagttcttc cgcggagtaa
      361 tgcgacgcga catccagccg tcgaagcccc attttgatgt actcgctgtt gatctcaata
      421 ccaatgaatt ttcgtccgct ggcgatggct acggcaccgg tagtaaagct accggcaaac
      481 gggtcgagaa cgatatcgcc tgggttggaa gaggcgagaa taatgcgttt cagtaaggct
      541 ttcggttttt gcgtcgggtg gttctcatat tcatccatca aataacgcac gcgcggaaaa
      601 tcccagacgt tacccggtac tttttgatga ttgtatggct gtggaggatt tttgcgataa
      661 tagatcaacg cgcgctgcga tccggttttg gcttctacca gaatagcatc accgttgaat
      721 gtgtagttct ttgcgtcttt caccatcatc aggatgggtt cgtacatgga gccgtagtgt
      781 tttttcgcct gtactccaga actgtcatat gaccagacga tgcgactttt gatggtaaaa
      841 agcttgcggc actggagatc gataaagggc atgttttccg tactgttcat aatgtacatg
      901 ctgccctgct ttttcagaac gcggtggcac tctgcaatca cttcaaacag ccagtcgata
      961 aacagatctt ctttccaggc ttcgatcaga ccatcaaaat ttttaccgat gttatacggt
     1021 gggtcggcaa agagcagatc gacgctttca gtaggtagct ttttaagttc ggcaagggca
     1081 tcaccgtgaa taatggtctt agcttcatta ccaaaccggg tcggttcaca tcctgttctc
     1141 atggtcactc cctttgtgac acctataaaa aaggcgcttc cctatgccga gtagcgcctt
     1201 tttaatcaag catttagcta acctgaatta gttcatgccg tattttttca attttttacg
     1261 cagcgtacca cggttgatgc ccatcatcag cgcagcacgg gtctggttac cacgggtgta
     1321 ttgcatcacc atgtccaaca ggggctgttc tacttcagcc agtaccagct catagaggtc
     1381 attcacatcc tgaccattca gttgagcaaa atagttcttc agtgcctgtt taaccgagtc
     1441 acgcaggggt ttttgggtta cctgatcctg agagttaacg gtagaaacgg tcagtacgtc
     1501 agaatttacg cgttgttcga acatagttct gtcagctctt tatttctgtt tacgcaaaat
     1561 tttcgaagta tgcctccaac gcctccagct gttcgctggc atcctcaatg gcgttgaatg
     1621 tgcgccgaaa ctggtcattt ggagcgtgtt cctggagata ccaggaaacg tgtttacgtg
     1681 caattcggta cccttttgcc ggaccataaa agtcatgcag ttcccgaacg tgcgcgcaaa
     1741 gcaagcgctt aacctctgcc aaaggcagcg ggggcagcaa ctccccagtg tccagataat
     1801 gctggatttc ccgaaagatc cagggtcttc cctgagctgc gcggcctatc atcagggcat
     1861 ccgcccctgt atagtcgagc acagctctgg ctttaagcgg gtcagtaatg tcgccattcg
     1921 cgataaccgg aatggaaact ttctgcttaa ctgcccgaat actgtcgtac tcagcttctc
     1981 cattgaacaa acaggcgcgt gtacggccat gaatggtcag agcctgaatg ccacagtctt
     2041 cagccagttg ggcaatctct tcgcagttac ggtgttccgg tgcccagccg gtgcgaatct
     2101 tcagggtaac aggaacgtcc actgcattga cgacctcggt aaggatcgat ttaacgacat
     2161 ccgggtactg caagagggct gaacctgcga gcttgcgatt cactttttta gccgggcaac
     2221 ccatattgat atcaataatc tgggcaccgc tttccacgtt aatacgtgct gcatctgcca
     2281 tttctttcgg agcgctacca gcaatttgca cggtgcgaat accgggttca tcaatgtgca
     2341 ccatccgtaa acgagatttg tcgctttccc aaacctgtgg gttagaagac atcatctcgg
     2401 atactgtcaa tccggctccc atctcgtagc acaacgtccg aaaaggtctg tctgtaatgc
     2461 cagccatggg cgctgcgatc aggcgatttc tgagctgata ttgtccgatg cgcatgagtt
     2521 aagaaatgac catactgtga ctgcaaggcg gcgtatatta cgcatttttt gcacgagatg
     2581 aaaggccaaa ctttgaccaa tcctctgaga tggatcaaag aattgcattt aaaatgagcg
     2641 tggtgcgata attactcata aaaatcatca tattagaaaa tagtgactaa aatttacact
     2701 caaagaaatt tgagtaagtt ctcaattttt ctttatgaat gaaaattttg gcacgcaaat
     2761 ttgcgtaaat aatcggcaaa tttacgtgcc ttttgtgagc ttgctcgcac ttcgccccgc
     2821 gtcaccctac ggcgatgcga aggttaattc ttacgaccgg taatacggca ccactcttct
     2881 ttttccacga ccgggtccag tgcgaagcta tcggcataag cttcacaaac gctctctgcc
     2941 tggcttgcca gaataccgga aaggcccagc aaaccgcctg aaaccggcag gacgctgatt
     3001 aacggtgcca gttcacgtaa tgggcctgca aggatgttag cgaccaccac gtcggctttc
     3061 atttcttctg gctgatcttt cggtaagtag agttccagac ggtcagaaac gccattacgt
     3121 tcggcgttat cgcggctggc ctgaatcgcc tgcggatcga tatcaatacc aatggctttt
     3181 gctgcaccca gtttcagcgc cgcgatcgcc agaatgccgg aaccacagcc aaagtcgatg
     3241 actgttttac cggttaaatc gaggctgtcg agccattgca ggcacagaga ggtggttgga
     3301 tgggtacccg taccaaacgc cagccctgga tctaacatca cgttgacggc gttttcgtcc
     3361 ggcacatcac gccagctagg gcagatccac agtcgttcac caaagcgcat cgggtggaaa
     3421 ttatccatcc attcgcgctc ccagtcttta tcttctagtt gttcggtttt atgcgcgaag
     3481 cctgcgccga gcagcggatg gttttccaga atcgccacca cgtcgttcat atcggtttca
     3541 gcgtcgaaca gaccaatcac atcggtgtcg ccccacaggc gcgtttcgcc cggcagcggc
     3601 tcaaatactg gcgtatcgtg ggtatcctga aaagtgatag aaacggcacc cgcttccatc
     3661 agcgcatcgc taagatcttc cgcgttcgcg ccggtggtgt tcagtttcag ttggatccaa
     3721 ggcatggcaa aactctttat ttatcagtag tcaaaacggt agcttgcggg acggatgtac
     3781 cgaaacggtt tccgaccagg aaagccagca aacttagtag taacgagggc acgatagggt
     3841 ggaagcccag gtactgaata ttcagcgtcg cgagtacggc atacagcacg ccgccaacga
     3901 tcatcgcact tagcgcgcct ttggcgttgg cgcgttccca gtaaagaccc agcaccagcg
     3961 gccacaggaa aacggcttcc agcccaccga aggccagcaa attcagccag atgatcattt
     4021 ctggcggctt ccaggcggca agcagcagca acgcgccgag aactaacgta attaccgccg
     4081 acatccgctt cagacgcgtc tcgttttgca tttgatccgg acggatattc agatagagat
     4141 ctttaatgat cgtagcggaa ctttgcagca gttgggcgtt aattgtcgac atgatcgcag
     4201 ccataggtgc agccagaaag atcccggcag caaaaggtgg cagcactttt accattaacg
     4261 tcgggatcac cagatccggt acggtgagat cggggatcac tgcacgacct aacgctccgg
     4321 ctaggtgcat accgaacatc aggattgcga ctacaatcgt accgatgatg atcccccggt
     4381 gtactgcttt gctgtcttta taagagatac agcgcaccgc agtatgcggc aggccaatca
     4441 cgccaaagca caccagtacc cagaacgacg tcataaaggc aggcgacaga atatcgtcag
     4501 cgccttgtgg cgtaaccagt tgcggatcga tggtttgcaa ggtctgtact gcgttgctta
     4561 agccgccagc ggcatgtacc acgccaataa gcagcacaac ggtgccaatc agcatcacaa
     4621 gcccttgcat ggtgtcgttc agcacgctgg cgcgaaagcc accaaaggcg gtatataacg
     4681 caatgctgat accaaaaatc agcagcccgg tttcataagg aatacccgcc gcggtttcca
     4741 gcaggcgcgc accgccgata aactgcacgg tcatcgcacc aacgaacgca accagcaaac
     4801 tcaaactcgc cagccacacc agaagacgac tctggtagcg ggcaaacagc atatcgttca
     4861 gcgtcactgc attgtagcgg cgcgcaagaa tcgcaaactt cttgccgaga ataccgagtg
     4921 aaagccagac agcaggaagc tgaatcatcg ccagcaatac ccagcccagc ccgtatttat
     4981 aagcagctcc tggcccgccg ataaacgaac tggcactgat ataggtcgcg gtgagcgtca
     5041 tcgccagcac aataccgccc atagagcggc tgccgaggaa atactcatta aggaaggtgc
     5101 cggtgctccg tttacgcatc gcataaaccg agataccgaa caccaccacc agataggcga
     5161 ccagcggtag aattacttca agctgcatcg tcatcctcca gtgggatatc gcgatagata
     5221 aatttcacca tcgcccagca cagtccaata aacagtagcg gcgtcaggat gcaggccatc
     5281 tcaaaccagc gcggaaagcc ggtaaaaccg ggggcaacgc caggtaagta agcatctact
     5341 aaccaaactg ccagatacaa aagggtcagc cccagcgccc agcgcgcctc tttatgggcc
     5401 tgaacaaaac gagtgtccat tttttgtccc tgatgggtga agaaagcgag gattgtacct
     5461 tatgggggtt gtcgatcccc agtaataaaa aaggccggaa aatccggcct tttgacgctt
     5521 ttgcagtctt atttttcctg aagaccgagt tttttctcca gatagtggat gttagtgcca
     5581 ccatgctgga agttctcgtc attcatgatg cggatctgca gatcaacgtt ggttttgata
     5641 ccgtcgatga tcagctcctg cagcgcattc ttcatgcggg caatcgccac gtcacggttt
     5701 tcaccgtagc aaatcagctt accgatcatt gagtcatagt acggcggtac ggtgtagccc
     5761 gcgtagatat gagactccca acgtacgcca aaaccgccag gtgcgtggaa acgggtgatt
     5821 ttgcctgggc ttggcaggaa ggtgttcgga tcttcggcgt tgatacgaca ttccaccgca
     5881 tggccgcgaa cgtgaacttc ttcttgcttg atcgacagcg gttgaccggc agcgatacgc
     5941 agctgttctt tgatcaggtc aacgccggtg atcatttctg taaccgggtg ttctacctga
     6001 atacgggtgt tcatttcgat gaaatagaac tcgccgtttt cgaacaggaa ctcgaaagta
     6061 cctgcaccgc gatagccgat atcaacacac gctttagcgc aacgttcgcc gatgtagcga
     6121 cgcagttccg gggtaatgcc cggtgctggt gcttcttcga ccactttctg gtggcggcgc
     6181 tgcatggagc agtcacgttc cgccagatag atagtgttgc cctgaccgtc agccagtacc
     6241 tgaatctcga cgtggcgagg attctccagg tatttctcca tgtaaaccat atcgttgctg
     6301 aaagcagctt tcgcttccgc acgggtcatg gagatggatt gtgccagttc agcgtcgccg
     6361 cgcactacgc gcataccgcg accgccgccg ccgccggagg ctttgataat caccggataa
     6421 ccaatgcgtt tagcaatggc gcggttttta tccatatcgt cgcccagcgg gccgtcagaa
     6481 ccaggtacgc aaggaacgcc cgcttttttc atcgccgcga ttgcggatac tttgtcgccc
     6541 atcaggcgaa tggtttctgc tttcgggccg atgaagataa agccggagcg ttcaacctgc
     6601 tcggcaaagt tggcgttctc ggagaggaag ccgtaacccg gatggattgc tactgcgccg
     6661 gtgatttcag cggcgctgat gattgccggg atgttcagat aactttgtac tgacggagca
     6721 gggccaatac agaccgtttc atctgccagt aatacgtgtt ttagatcgcg atccgcgctg
     6781 gagtgcacag cgacagtctt gatgcccagt tctttacagg cacgaagaat acgcaatgca
     6841 atctcgccgc ggttggcaat aacaatttta tccagcatgt tcgcctcgtt actcgatgac
     6901 gaccagcggc tcgtcaaatt ctaccggttg tccactttcg accagaattg ctttcacggt
     6961 accggatttg tccgcttcga tctggttcat cattttcatg gcttcaacga tgcacagggt
     7021 atcgcccacg ttgactttct gacccacttc gatgaacgct tttgcgtccg ggcttggggt
     7081 gcggtagaaa gtaccaacca tcggggaacg tacgatgtga ccactgattt ccgctgctgc
     7141 tggcgcttcc atggaaggaa cggtcgccgg agcggctgcg ttagattgag ctggctgctg
     7201 catcattggt gcagcgtaag cttgttgcat cacagggaaa cttgcggcag gagctgcacg
     7261 gctaatgcgt actgactctt cgccttcaga aatttccagt tcggagatgc ctgattcttc
     7321 aaccagctcg atcagttttt taatcttacg aatatccatg agtgggttcc gtactctttg
     7381 tttagtgtga ttgtgacagg cgtttcaccg ccgtctgtaa agcgtatgcc ccgacagctg
     7441 tatgcatagc gataaattcc agcaggccgg gacgccggtc tattttgccc cagagtgtca
     7501 atgttagact tgacggacat tgtgcagcga tctgctgcta tcctccggca aaaaacaaaa
     7561 tataccttcg ttgcgccaat gtcacctttt ccgcagcgca aaaactgcgt cagggaggac
     7621 gaggtcgcac attataacga tttcgtagca attggcagct aaatactggt cttatcaggg
     7681 aagataatca acagctaaca tgtaaataac cttcaacacc gtgtaatttg caacaagccg
     7741 cacaattcac gaaattagcg ccatcatcga cggaacttct tataacgtaa ggtgaaaagc
     7801 tgaaaagcca gccctgcgaa gatgaggggc tgcaatgaga taatcttcac agaccacaaa
     7861 taatgtaggg atgccaggat cgcgacaaga tagacgacat tacgcagcgg ttgctgggga
     7921 ttacccaatt ttcactgcat cgtctggata gatgtgaatg ctaaagcacg caaaatcaac
     7981 caactgacaa ttctcagcat taaataggtt cgagtggtta atttgtagtc aaataaaatc
     8041 agattattca ccccatggta ccagaaatca gtaacaggtt aagcgtagtg tcgcccaggc
     8101 aaagcaccat aaacctaaca ggcgacggag gcgtatcaat aatggctgtt tagaggagta
     8161 tcaatgcgca tcaatttgtg gtcagtcgaa atcatgactt tccagtttgg atgatgttgt
     8221 acaacttcct gaccaaggtg ttcgacgaaa gctgtccggc taaccgcttt tcccgttaac
     8281 cgattgaata atgatgtcaa aatgagttcc ctctcttatt attccttgct aaatggttag
     8341 ttaaccttca ccagcgtgcg accctggatc tggttattaa tgatggcctc ggcaaagttc
     8401 ggtgcctctg acagagatat ctcttttgcc gcctgggtat agaatgattc cggtaaatcg
     8461 gcgaccagtc gctgccaggc ttgtgcgcgg cgttctggtg gcgtcattac tgaatccacc
     8521 ccttgcaaac ggacattacg cagaataaat ggcatgaccg tgcttggcag agtaaaacca
     8581 cccgccagac cacaggccgc cacgcagccg ccgtaattca tttgcgccag cacttttgcc
     8641 agcactttgt cgccaacagt gtcaattgcc ccagcccaga cttgtttttc cagaggacgg
     8701 gattcggcaa actcatcacg agggagaata cggctggcac ctaaactttt cagatattca
     8761 tgggtacttt cgcgaccgga aacggcaacg acctgataac ccaacttatg cagcagcgcc
     8821 acggcggtac tgccgacgcc accactggca cccgtcacga caatctcccc gtcctgcggg
     8881 cgaacaccgg catcttccag cgccatcaca cacagcatgg cggtaaaacc ggcagtaccg
     8941 ataatcattg ctttacgcgc gtccagccct tgcggcatgg caaccagcca gtcacctttc
     9001 actcgcgcct gctccgccag cccaccccag tggttttcac caacgcccca gccagtgagt
     9061 aacacctcct gaccggcatg aaaacgcgga tcttcgctgg tgcgtacagt tccggcaaaa
     9121 tcgatcccag gaatcatcgg aaaattacgg atgatttttc ccttaccggt aatcgccagc
     9181 gcatctttat agttcaggct cgaccagtga acatcgaccg tgacatcgcc ctccggcagg
     9241 cgactttcgt ccagagtctg tactgatgcg agagttttgc cgtcctgctg ttctaaaagt
     9301 aacgcctgca taagtggtcc tcatgtgcat gatggattgg aaaataattt ctgaagacta
     9361 tactcgctaa tggaaaagca aagccgatga agcgcaataa attgcgagat aaatctgatt
     9421 tgctagtatg cccgcttcct cactatcgga gttaacacaa ggatgagatt aacgacgaaa
     9481 ttttcggcct ttgttacgct gctcaccggg ttaacaattt ttgtgacttt gctgggctgt
     9541 tcgctaagtt tctacaacgc cattcagtat aagtttagtc atcgcgttca ggcggtggcg
     9601 acggcgattg atacccacct tgtgtcgaat gacttcagcg cattaaggcc acaaattacc
     9661 gaattaatgg tgtcagcaga tatcgttcgt gtagacctgc tgcatggtga taaacaggtt
     9721 tataccctgg ccagaaatgg tagttatcgt ccggttggca ccagcgatct gtttcgtgaa
     9781 ctgagcgttc cgttgataaa gcatccgggg atgtcgttgc gtctggttta tcaggatccg
     9841 atgggcaact atttccattc gttgatgacc accgcgccgc tcacgggggc gattggcttt
     9901 atcattctta tgctctttct ggcggtacgc tggttacaac ggcaacttgc cgggcaagaa
     9961 ttgctggaaa cccgggctac tcgtatctta aacggtgagc gtggctctaa tgtgttggga
    10021 accatctatg aatggccgcc cagaaccagc agtgcgctgg atacgctgct tcgtgaaatt
    10081 cagaacgcac gcgaacaaca cagccgtctt gatacgctga tccgctctta tgccgcacag
    10141 gacgtgaaaa ccggcctcaa taaccgactc tttttcgata atcagttagc aacgttactg
    10201 gaagatcagg agaaagtagg tacccacggg atcgtgatga tgattcgtct gccggatttc
    10261 aatatgttga gcgatacctg ggggcacagc caggttgaag aacagttctt cactctgacg
    10321 aatctgctgt cgacatttat gatgcgctac cctggcgcac tgctggcgcg ttaccaccgc
    10381 agtgattttg ctgcgctgtt accgcaccgg acgttaaaag aggcagagag catagccagt
    10441 cagttaatca aagccgtcga taccttgccg aacaataaaa tgctcgatcg cgacgatatg
    10501 atccacattg gtatctgtgc ctggcgtagt ggtcaggata ccgagcaggt aatagaacat
    10561 gcagagtctg ccacgcgtaa tgcgggattg cagggtggca atagctgggc tatttatgat
    10621 gactcgttgc ctgaaaaagg acgcggtaat gttcgctggc gtacgcttat cgagcaaatg
    10681 ctcagtcgcg gcggcccgcg cctttatcaa aaaccggcgg ttactcgcga aggtcaggtt
    10741 catcatcgcg aactcatgtg ccgcatcttc gatggtaatg aagaggttag ctcggcggag
    10801 tatatgccga tggtcttgca gtttggctta tcggaagagt atgaccgtct gcaaatcagc
    10861 cgtcttattc cactattgcg ttactggcca gaggaaaatc tggcgattca ggttaccgtt
    10921 gagtcgctga ttcgtccgcg ttttcagcgt tggctgcgcg atacgttaat gcaatgtgaa
    10981 aaatcacaac gaaaacgcat aattattgaa cttgcagagg ccgatgtagg tcaacatatc
    11041 agtcgtttac aacctgttat tcgtttagtg aatgctttag gggtacgggt agccgtcaac
    11101 caggctggtt tgacgctggt aagcaccagt tggatcaaag aacttaatgt tgagttactc
    11161 aagctccatc cggggctggt caggaacatt gagaagcgaa cggagaacca gctgctggtt
    11221 caaagcctgg tggaagcctg ctccgggacc agcacccacg tttacgccac cggcgtgcgt
    11281 tcgcgaagcg agtggcagac cctgattcag cgcggtgtta caggcgggca aggggatttt
    11341 ttcgcgtcct cacagccact tgatactaac gtgaaaaaat attcacaaag atactcggtt
    11401 taacctgccg tttaatccgt tttcacgtag aataacgcgc gctgcgtctc atgggagtgt
    11461 gcttgtctgc tcgccagatt gttgcagcac atatgcagat gaatgacctt acgcggttgc
    11521 aaacaggcga ggaatgctgc tgatgcatta agcctttctg gactcaggca gagatttgta
    11581 acaaaggaaa cgaactgcac taattttcac cgtagcagat gatttttgcg ccttgtcgct
    11641 gctgcgtgtg gttggtaaag taagcggatt ttcttttccg ccccagcttt caggattatc
    11701 ccttagtatg ttgaaaaaat ttcgtggcat gttttccaat gacttgtcca ttgacctggg
    11761 tactgcgaat accctcattt atgtaaaagg acaaggcatc gtattgaatg agccttccgt
    11821 ggtggccatt cgtcaggatc gtgccggttc accgaaaagc gtagctgcag taggtcatga
    11881 cgcgaagcag atgctgggcc gtacgccggg caatattgct gccattcgcc caatgaaaga
    11941 cggcgttatc gccgacttct tcgtgactga aaaaatgctc cagcacttca tcaaacaagt
    12001 gcacagcaac agctttatgc gtccaagccc gcgcgttctg gtttgtgtac cggttggcgc
    12061 gacccaggtt gaacgccgcg caattcgtga atccgcgcag ggcgctggtg cccgtgaagt
    12121 cttcctgatt gaagaaccga tggctgccgc aattggtgct ggcctgccgg tttctgaagc
    12181 gaccggttct atggtggttg atatcggtgg tggtaccact gaagttgctg ttatctcctt
    12241 gaacggcgtg gtttactcct cttccgtgcg cattggtggt gatcgtttcg acgaagctat
    12301 catcaactat gtgcgtcgta attacggttc tctgatcggt gaagccaccg cagaacgtat
    12361 caagcacgaa atcggttcgg cttacccggg cgatgaagtc cgtgaaatcg aagttcgtgg
    12421 ccgtaacctg gcagaaggtg ttccacgcgg ttttaccctg aactccaacg aaatcctcga
    12481 agcactgcag gagccgctga ccggtattgt gagcgcggta atggttgcac tggaacagtg
    12541 cccgccggaa ctggcttccg acatctccga gcgcggcatg gtgctcaccg gtggtggcgc
    12601 actgctgcgt aaccttgacc gtttgttaat ggaagaaacc ggcattccag tcgttgttgc
    12661 tgaagacccg ctgacctgtg tggcgcgcgg tggcggcaaa gcgctggaaa tgatcgacat
    12721 gcacggcggc gacctgttca gcgaagagta atcggatgca ggcaggggaa gcgtctgttt
    12781 accctgcctg gtctgatacg agaatacgca taacttatga agccaatttt tagccgtggc
    12841 ccgtcgctac agattcgcct tattctggcg gtgctggtgg cgctcggcat tattattgcc
    12901 gacagccgcc tggggacgtt cagtcaaatc cgtacttata tggataccgc cgtcagtcct
    12961 ttctactttg tttccaatgc tcctcgtgaa ttgctggatg gcatatcgca gacgctggcc
    13021 tcgcgtgacc aactagaact tgaaaaccgg gcgttacgtc aggaactgtt gctgaaaaac
    13081 agtgaactgc tgatgcttgg acaatacaaa caggagaacg cgcgtctgcg cgagctgctg
    13141 ggttccccgc tgcgtcagga tgagcagaaa atggtgactc aggttatctc cacggttaac
    13201 gatccttata gcgatcaagt tgttatcgat aaaggtagcg ttaatggcgt ttatgaaggc
    13261 cagccggtca tcagcgacaa aggtgttgtt ggtcaggtgg tggccgtcgc taaactgacc
    13321 agtcgcgtgc tgctgatttg tgatgcgacc cacgcgctgc caatccaggt gctgcgcaac
    13381 gatatccgcg taattgcagc cggtaacggt tgtacggatg atttgcagct tgagcatctg
    13441 ccagcgaata cggatattcg tgttggtgat gtgctggtga cctccggtct gggcggtcgt
    13501 ttcccggaag gctatccggt cgcggttgtc tcttccgtaa aactcgatac ccagcgcgct
    13561 tatactgtga tccaggcgcg tccgactgca ggactgcaac gtttgcgtta tctgctgctg
    13621 ctgtggggcg cggatcgtaa cggcgctaac ccgatgacgc cggaagaggt gcatcgtgtt
    13681 gctaatgaac gtctgatgca gatgatgccg caggtattgc cttcgccaga cgcgatgggg
    13741 ccaaagttac ctgaaccggc aacggggatc gctcagccga ctccgcagca accggcgaca
    13801 ggaaatgcag ctactgcgcc tgttgcgccg acacagcccg ctgctaatcg ctctccacaa
    13861 agggctacgc cgccgcaaag tggtgctcaa ccgcctgcgc gtgcgccggg agggcaatag
    13921 tggcgagcta tcgtagccag ggacgctggg taatctggct ctctttcctc attgcgctgt
    13981 tgctgcaaat catgccctgg ccggataacc tgattgtttt ccggccaaac tgggtgttac
    14041 ttatcttgtt gtattggatc ctggccttgc cccatcgcgt aaatgtgggc acaggttttg
    14101 tgatgggtgc catactggat ctgatcagcg gctcgatgct tggcgtacgc gtattggcga
    14161 tgagcatcat tgcttacctg gtggcgctga aataccagct tttccgcaac ctcgcattat
    14221 ggcagcaggc gctggtcgtc atgttgcttt cgctggtggt ggatattatt gttttctggg
    14281 cagagttttt agtgattaac gtctctttca gaccggaagt gttctggagt agtgtagtca
    14341 atggggtgct ctggccgtgg attttcttgc tgatgcgtaa agtccgtcag cagtttgccg
    14401 tgcaataaag gtttctatga cttctctgta tttagcttcc ggttctccgc gtcgtcagga
    14461 gttacttgcg caacttggcg tgacctttga acgtattgtt acgggcattg aggagcagcg
    14521 tcagccgcag gagagcgcgc agcagtatgt tgtgcgtctg gcacgcgaga aagcacaggc
    14581 gggtgtcgcg caaacggcac aggatctccc ggtgctgggt gcggatatta tcgttatcct
    14641 gaacggagaa gtgctcgaga aaccgcgcga cgcagagcat gcggcgcaga tgttgcgcaa
    14701 attatcgggt cagacccatc aggtgatgac ggcagtggcg ttggccgaca gccagcatat
    14761 tctcgattgc ctggtggtca ccgatgtgac tttcagaacg ttaacagacg aagacatcgc
    14821 gggctatgtc gccagcggtg aaccgttaga taaagcaggt gcatacggta ttcaggggct
    14881 gggtggctgt tttgtcagga agataaatgg cagctatcac gccgtagtcg gcttaccgct
    14941 ggttgaaacg tatgaattat taagtaattt taacgcactg cgtgagaaaa gggataaaca
    15001 tgacggctga attgttagta aacgtaacgc cttcggaaac gcgagtggcg tatattgatg
    15061 gcggtattct gcaggaaatt catattgaac gtgaggcgcg acgcggaata gtaggcaata
    15121 tctacaaggg tcgtgtaagt cgtgtacttc cgggtatgca ggcggctttt gtagatattg
    15181 ggctggataa agccgcgttt cttcatgcat ccgacatcat gccgcatacc gaatgtgtgg
    15241 cgggtgaaga acaaaagcaa ttcacggtgc gcgacatctc ggaactggtt cgtcaggggc
    15301 aagatctgat ggtgcaggtg gtgaaagatc cgcttggcac taaaggtgcg cgcctgacca
    15361 ccgatatcac gctgccttct cgctatctgg tatttatgcc aggggcttct cacgttgggg
    15421 tttcccaacg aattgaaagc gaatcagaac gtgaacgcct gaaaaaagtg gtcgcagagt
    15481 attgcgacga gcagggcggg tttatcatcc gcaccgcagc ggaaggggtt ggcgaggctg
    15541 aactggcctc cgatgccgct tatctgaaac gcgtctggac caaagttatg gagcgtaaaa
    15601 agcgcccgca gacccgttat cagctgtacg gcgaactggc gctggcgcag cgtgttctgc
    15661 gtgattttgc cgacgccgaa ctggaccgca ttcgcgttga ctcacgcctg acttacgaag
    15721 cattgctgga gtttacctcg gagtacattc ccgagatgac cagcaagctg gagcattaca
    15781 ccggacgcca gccgattttc gatctctttg atgtcgaaaa cgaaatccag cgagcgttgg
    15841 aacgcaaagt agaactgaaa tccggtggtt atctgattat cgaccagacc gaagcgatga
    15901 ccaccgtgga catcaatacc ggagcgtttg tcggtcatcg caatctggac gacactattt
    15961 tcaataccaa tattgaagcg acgcaggcta tcgctcgcca gttacggttg cgtaatctgg
    16021 gcgggattat cattattgat ttcatcgata tgaataatga agatcaccgc cgccgcgtgc
    16081 tgcattcgct ggagcaggcg ttgagcaaag accgggtgaa aaccagcgtt aatggttttt
    16141 cggcgctggg gctggtggag atgacgcgta aacgcacccg cgaaagcatt gagcacgtac
    16201 tgtgtaacga atgcccaacc tgccacggtc gcggaacggt gaaaaccgtg gaaacggtat
    16261 gctatgaaat catgcgcgag attgttcgtg tccaccatgc ttacgactcc gaccgtttcc
    16321 tggtctatgc ttctccggca gtagccgaag ccttgaaagg cgaagagtca cactcgctgg
    16381 cggaagtgga aattttcgtt ggcaaacagg ttaaagtaca aattgaaccg ctctataacc
    16441 aggagcagtt tgacgtcgta atgatgtaaa cagatgctgg gccgccatcc ggcaaagggt
    16501 ttttgagtca catttttagc agacaaggag tgacgggtga ggcgattgcc ggggatttta
    16561 ctgcttactg gagccgcgct cgttgtgatc gctgccctgc tggttagcgg cctgcgtatt
    16621 gctttaccgc atcttgacgc ctggcgtccg gaaatcctca acaaaataga atccgcgact
    16681 ggcatgccgg tagaagccag tcagctctca gccagctggc agaattttgg cccgacgctt
    16741 gaagcacacg acatccgtgc agaactaaaa gatggcggcg aattttcggt taaacgcgtt
    16801 actctggcgc tggatgtctg gcagagcctg ttacatatgc gctggcagtt tcgcgacctc
    16861 actttctggc agctgcgctt tcgcaccaac actcctatca ccagcggtgg tagcgatgac
    16921 agtctggaag ccagtcacat cagcgatctg tttcttcgtc aatttgacca tttcgatctc
    16981 cgcgacagtg aagtcagttt tctgacgcca tccggtcaga gcgccgagct ggcgatccca
    17041 caactcacct ggctgaacga tccacgtcga caccgtgcgg aaggcctggt aagcctctcc
    17101 agccttaccg ggcagcacgg cgtgatgcag gtgcgcatgg atttgcgcga tgatgagggg
    17161 ttgttaagca atggtcgcgt ctggctacag gcggatgaca tcgacctgaa gccgtggctc
    17221 ggtaaatgga tgcaggacaa tattgctctg gaaacggcac agttctccct tgaaggctgg
    17281 atgacgatcg acaaaggcga tgtaaccggc ggtgacgtct ggctgaaaca gggcggtgcc
    17341 agctggttgg gcgagaagca aacgcatacg ctgtcggtgg ataatctgac cgcgcatatt
    17401 acgcgtgaaa atccgggctg gcagttctct attcccgata cacggatcac gatggacggc
    17461 aaaccctggc cgagcggagc attgacgctg gcctggatac cggaacagga cgttggcggc
    17521 aaagacaata aacgcagtga cgaactccgg attcgcgcta gtaatctgga gctggcaggc
    17581 ctggagggca tacgcccgct ggccgcgaaa ctttcacctg cactgggtga tgtttggcgc
    17641 tccacacaac cgagcggcaa gattaacact ctggcgctgg atatcccgct tcaggcggca
    17701 gacaagaccc gttttcaggc atcgtggagc gatctggcct ggaagcaatg gaaattatta
    17761 ccgggtgcgg aacacttctc cgggacgctt tccggcagcg ttgaaaatgg tttgcttacc
    17821 gcgtcgatga agcaggcaaa gatgccttac gaaacggtat tccgtgcgcc actggaaatc
    17881 gccgacggcc aggcaactat aagctggctg aacaatgaca aaggtttcca gctggatggg
    17941 cgtaatattg acgttaaagc caaagccgtc catgcgcgcg gcggttttcg ttacctgcaa
    18001 cctgctaacg atgaaccctg gctgggtatt ctggctggca tcagtaccga tgatggttca
    18061 caagcctggc gctatttccc ggaaaacttg atgggtaaag acctggttga ttatttaagt
    18121 ggtgcgattc agggcggtga agcggataac gcgacgctgg tttatggtgg caatccgcaa
    18181 ctcttcccct ataaacacaa cgaaggtcag tttgaagtgc tggtgccgct gcgcaacgcg
    18241 aagtttgcct tccagccgga ctggcctgca ttaactaacc ttgatattga actggacttt
    18301 attaacgacg gtttatggat gaaaaccgat ggcgttaatc tgggcggcgt gcgcgcgagt
    18361 aatctcaccg cagtgatccc tgactactca aaagaaaaac tgctgattga cgctgacatt
    18421 aaaggtccgg gtaaagccgt tggcccttac tttgatgaga caccgctgaa agattctctg
    18481 ggtgcgaccc tgcaagaact ccagctcgac ggcgatgtga atgctcgctt acatcttgat
    18541 atcccgctga acggcgaact ggtaaccgcg aaaggtgaag tgacgctgcg taataacagt
    18601 ctgtttatca aaccactcga cagcatcctg aaaaatttga gcggtaaatt cagctttatc
    18661 aatggcgatc tgcaaagtga accactgaca gcaagctggt ttaatcagcc gttgaacgtg
    18721 gatttttcca ccaaagaagg ggcaaaagcc taccaggttg cggtgaatct caacggtaac
    18781 tggcaaccgg cgaaaaccgg cgttctgcct gaagcggtga acgaagcatt gagtggcagc
    18841 gtggcgtggg atggtaaagt gggcattgat ctgccttatc atgctggcgc gacctataac
    18901 gtagagctga acggcgatct gaataatgtg agtagtcact taccttcacc gttagccaaa
    18961 cctgcgggtg aaccactagc ggtaaacgtt aaggttgatg gcaatctcaa cagctttgaa
    19021 ttaaccggac aggctggtgc ggataaccat ttcaatagcc gctggttgct tggtcaaaag
    19081 ctgacgctcg atcgtgctat ttgggcggca gacagtaaaa cgctcccgcc gttgccggaa
    19141 caaagtggcg ttgaacttaa tatgccgccg atgaatggtg ccgagtggct ggccctgttc
    19201 cagaaaggcg ctgcggagag tgtcggtggt gcagcgagtt tcccacaaca cataacatta
    19261 cgtacgccta tgttgtcact gggaaatcag caatggaata acctgagtat tgtttcgcaa
    19321 ccgacggcaa atggcaccct gattgaggcg caagggcgtg aaatcaacgc cacgctagcg
    19381 atgcgtaata acgcgccgtg gctggcgaat atcaaatatc tttattacaa cccgagcgtg
    19441 gcgaaagctc gtggtgattc aacgccgtca tcacctttcc cgacaacgga gcgcattaac
    19501 ttccgtggct ggccggacgc acaaatacga tgcacagagt gctggttctg ggggcaaaaa
    19561 ttcggtcgca ttgacagtga tatcaccatt tctggcgata cgttaacgct gaccaatgga
    19621 ctgattgata ctggtttctc gcggcttact gccgatggtg aatgggttaa taatccgggg
    19681 aatgaacgta cctcgctgaa aggaaaactg cgtgggcaga aaattgatgc cgccgcagaa
    19741 ttttttggtg tcacgacgcc catacgccag tcgtcattta atgtggatta cgatttacac
    19801 tggcgcaaag caccctggca gccagatgaa gcgacgttga atggcatcat tcatactcaa
    19861 ctgggtaaag gcgaaattac cgaaatcaat accggacatg ccgggcaatt gctgcgctta
    19921 ttgagcgtag atgccctgat gcgtaagctg cgttttgatt tcagagacac ttttggcgaa
    19981 gggttctatt ttgactccat tcgcagcacc gcgtggatta aagacggcgt tatgcacacc
    20041 gacgacacgc tggtggatgg cctggaggcg gatatcgcca tgaaagggtc ggtaaatctg
    20101 gtacgtcgag acctgaatat ggaagcggtt gtcgcaccag agatttctgc gacggtgggc
    20161 gtggctgcgg cttttgcggt taaccccatt gttggcgcgg cagtgtttgc cgccagtaaa
    20221 gtgctggggc cgctgtggag caaagtctcc attttgcgct atcacatttc gggtccgctg
    20281 gatgatccgc aaatcaacga agtgttgcgc caaccgcgta aagaaaaagc gcaatgattt
    20341 gacgagggcg cgtaattgcc ccaatctcat atgataatcg ttgccaaagg ccaacgagcc
    20401 ggaacataac tgtaggccgg atagggcgtt cacgccgcat ccggcagccg taaaaaatcc
    20461 tctactgcag taactaacga gtagcaaaaa cgatgagtct gaacctggta agtgaacaat
    20521 tgctagcggc gaacggcctg aaacatcagg acttgttcgc gatcctcggt caactggccg
    20581 aacgtcgcct tgattatggc gatctctatt ttcagtcgag ctatcacgaa tcctgggttt
    20641 tagaagaccg cattattaaa gatggttctt acaacatcga tcagggcgtt ggcgtgcgtg
    20701 caatcagcgg tgaaaaaacc ggatttgctt acgctgacca aatcagcctg ctggcgctgg
    20761 aacagagtgc gcaagcggcg cgcaccatcg tccgtgatag tggtgatggc aaagtacaga
    20821 cgctgggcgc ggtagagcat agcccgttgt atacctcggt agatccgctg caaagcatga
    20881 gccgtgaaga gaagctggat atcctgcgtc gcgtcgataa ggttgctcgc gaagcggaca
    20941 agcgcgtaca ggaagtgact gccagcctca gtggtgtcta tgaattaatt ctggttgcgg
    21001 ccaccgacgg cacgctagcg gcggatgtcc gtccgctggt gcgtctttcc gtgagcgttc
    21061 tggtcgaaga agatggcaaa cgcgaacgcg gtgccagtgg cggcggcggt cgttttggtt
    21121 atgagttctt ccttgccgat cttgacggcg aagtccgtgc ggatgcatgg gcaaaagaag
    21181 cagtgcgtat ggcgctggtc aatctttctg ccgttgctgc accagcgggc accatgccgg
    21241 tagtacttgg cgcaggttgg ccgggcgtgc tgttgcatga agcggtaggt cacggtctgg
    21301 aaggcgactt caaccgccgt ggcacttcag tatttagtgg acaggtcggg gagctggtgg
    21361 cttcagaact gtgtaccgtg gttgatgacg gcacgatggt cgatcgccgt ggttcggtgg
    21421 cgattgatga cgaaggtacg ccaggccagt acaacgtgct gattgagaac ggcattctga
    21481 aaggctacat gcaggataaa ctcaacgcgc gtttgatggg gatgacgccg actggcaacg
    21541 gtcgccgtga atcctacgcc catctgccca tgccgcgtat gaccaacacc tatatgctgc
    21601 cgggtaaatc gaccccgcaa gaaattattg aatccgttga gtacggtatc tatgcaccga
    21661 actttggtgg cggtcaggtg gatatcacct ccggcaaatt cgttttctcc acttcagaag
    21721 catatctgat tgaaaacggt aaagtaacga agccggtgaa aggcgcaacg ttgattggtt
    21781 ccggtatcga aaccatgcag cagatttcga tggttggcaa cgacctgaaa ctggataacg
    21841 gcgtgggtgt ctgcggtaaa gaagggcaaa gtttgccggt tggcgtgggc cagccaacgt
    21901 tgaaagtcga taacctgact gttggcggta ctgcgtaata atttcattat tttcagaagg
    21961 ataattaatc tttctacgtg cacgaacggt cccctcgctc ctctggggtt agggtgaggg
    22021 gaacccgttg gcacaggttt gtagaacgta acagtacaat atgaattact tctctttccc
    22081 gcgcccgtgc atctcctgaa acaatttacc gacctcaaca aaataatccg tcagcgagtt
    22141 gatcacgacc tgtaccttca gcggcagctt atctttttcg gtatataacg cataaaccgg
    22201 gcgtggatct gactgataac gcggcagcag gatctccagc tccccacgat tgatctcgtt
    22261 gatcacccac atcagcggca cgtaggcgat cccggcaccc gccgttagcc agcgcaccag
    22321 cgtcatcgga tcattagtca caaatctccc ttgcgggatc aggcgagtcg agatcccttc
    22381 cggtgcaatc agttcaaatt cattgtcggg ccgcacgctg tattcaagcc atgaatggct
    22441 acttaaatcg gcgggttttt ccggtatgcc gtattgtgtg agatagcttt tcgcggcgca
    22501 caccaccatt ggcatcgcgc caagacggcg ggaaaacagg ctggaatcct gcaacgcgcc
    22561 gacgcggatc accacatcca gaccgtcggc aatcaggtcg ggggctggaa ttccggtaac
    22621 cagattggcg ctcaaacctg ggtattcttt cagcattttg gctgtcagcc cggcgagaac
    22681 attttgtgcc atagttgaag aacagccaat gcgtagcgtc ccgatggggg tgttattgaa
    22741 ggcatacagt tgctcatgaa catcctgcac ttcatgaagc atacgacggc agccctggta
    22801 gtaaattcta ccggcttcgg tcaggccaat gctgcgtgtg ctacggttta acagctttac
    22861 ctgcaactca tcttccagtt ttgacaccgt ctgactgatg gacgaaacgc tcatctgtag
    22921 ctgtctggcg gcggcggtaa aagagccaaa ttcaactact ttggcaaaca ccgacatgcg
    22981 ttttagtcgt tccattattc actctgactt aaaagtgatt tagatcacat aatatagata
    23041 acagcataac agttacgcta atatattaaa tatcaatcta cagcaatgtt gctctcgccc
    23101 ggctttccat gcccttctct gcggcgacag atgctgaaaa taataacgcc tgctctctct
    23161 atacaaccaa ggtcaacatg agtctgtttc ccgttatcgt ggtgtttggg ctgtccttcc
    23221 caccgatatt ttttgaattg cttttatcac tggcgatttt ctggctggtg cgccgggtac
    23281 ttgtgccaac aggtatctac gactttgtct ggcatccggc gttgttcaac accgcgctct
    23341 attgctgctt gttttatttg atatcgcgac tgttcgtttg aggttgaagt gaaaacacta
    23401 ataagaaaat tctcccgtac ggccatcacg gtcgtattag tcattctggc cttcatcgca
    23461 atttttaatg cctgggtcta ttacaccgaa tccccctgga cgcgtgacgc gcgctttagc
    23521 gctgacgtcg ttgcgatcgc gccggacgtt tctggactca ttacccaggt gaatgttcat
    23581 gataaccagc tggtgaaaaa aggacagata ctgttcacca tcgaccagcc gcgctatcaa
    23641 aaggcgcttg aggaagcgca agccgatgtt gcttattatc aggtactggc acaggagaaa
    23701 cgccaggagg ccggacgtcg taaccgtctc ggtgtgcagg cgatgtctcg cgaagagatc
    23761 gaccaggcta acaacgtact acaaacggtt ctgcatcagt tagcgaaagc gcaggcgacc
    23821 cgcgatctgg caaaactgga tcttgaacgc acggtgatcc gagcgccagc agatggctgg
    23881 gtgaccaacc tcaacgtcta taccggtgag tttattactc gaggatcaac ggcggttgcg
    23941 ctggtgaaac agaactcctt ctatgtactg gcctatatgg aagaaactaa gctggaaggg
    24001 gtgcgtccgg ggtatcgtgc agagatcacg ccgcttggca gtaacaaagt gctgaaaggg
    24061 actgttgata gtgttgccgc tggggtcacc aacgccagca gcacgcgtga cgacaaaggg
    24121 atggcgacca tcgactctaa ccttgaatgg gtgcgtttgg cgcaacgtgt tccggttcgt
    24181 attcgtctcg acaaccagca agagaacatc tggcctgcgg gcaccactgc tacagtggtg
    24241 gtcactggca aacaagatcg cgacgaaagc caggattcgt tcttccgtaa aatggcccat
    24301 cgcctgcgtg agtttggtta atcacgatgg gtattttctc cattgctaac caacatattc
    24361 gctttgcggt aaaactggcg accgccattg tactggcgct gtttgttggc tttcacttcc
    24421 agctggaaac gccacgctgg gcggtactga cagcggcgat tgttgccgcc ggtccggcct
    24481 ttgctgcggg aggtgaaccg tattctggcg ctattcgcta tcgtggcttt ttgcgcatca
    24541 tcggcacatt tattggctgt attgccggac tggtgatcat cattgcgatg atccgcgcac
    24601 cattattgat gattctggtg tgctgtatct gggccggttt ttgtacctgg atatcctcgc
    24661 tggtacgaat agaaaactcg tacgcgtggg ggctggccgg ttataccgcg ctgatcattg
    24721 tgatcaccat tcagccggaa ccattgctta cgccgcagtt tgccgtcgaa cgttgtagcg
    24781 agatcgttat cggtattgtg tgtgcgatta tggcggattt gctcttttct ccgcgatcga
    24841 tcaaacaaga agtagatcga gagctggaaa gtttgctggt cgcgcaatat caattaatgc
    24901 aactctgtat caagcatggc gatggtgaag ttgtcgataa agcctggggc gacctggtgc
    24961 gacgcaccac ggcactacaa ggcatgcgca gcaacctgaa tatggaatct tcccgctggg
    25021 cgcgggccaa tcgacgttta aaagcgatca atacgctatc gctgacgctg attacccaat
    25081 cctgcgaaac ttatcttatt cagaatacgc gcccggaatt gatcactgat actttccgcg
    25141 aattttttga cacgccggta gaaaccgcgc aggacgtcca caagcagctc aaacgcctgc
    25201 ggagagttat cgcctggacc ggggaacggg aaacgcctgt caccatttat agctgggtcg
    25261 cggcggcaac gcgttatcag cttctcaagc gcggcgttat cagtaacaca aaaatcaacg
    25321 ccaccgaaga agagatcctg caaggcgaac cggaagtaaa agtagagtca gccgaacgtc
    25381 atcatgcaat ggttaacttc tggcgaacca cactttcctg cattctgggc acgcttttct
    25441 ggctgtggac gggctggact tccggcagtg gtgcaatggt gatgattgcg gtcgtgacgt
    25501 cactggcaat gcgtttgccg aatccacgca tggtggcgat cgactttatc tatgggacgc
    25561 tggccgcgct gccgttaggg ctgctctact ttttggtgat tatccctaat acccaacaga
    25621 gcatgttgct gctgtgtatt agcctggcag tgctgggatt cttcctcggt atagaagtac
    25681 agaaacggcg actgggctcg atgggggcac tggccagcac cataaatatt atcgtgctgg
    25741 ataacccgat gactttccat ttcagtcagt ttctcgacag cgcattaggg caaatcgtcg
    25801 gctgtgtgct cgcgttcacc gttattttgc tggtgcggga taaatcgcgc gacaggactg
    25861 gacgtgtact gcttaatcag tttgtttctg ccgctgtttc cgcgatgact accaatgtgg
    25921 cacgtcgtaa agagaaccac ctcccggcac tttatcagca gctgtttttg ctgatgaata
    25981 agttcccagg ggatttgccg aaatttcgcc tggcgctgac gatgattatc gcgcaccagc
    26041 gcctgcgtga tgcgccgatc ccggttaacg aggatttatc ggcgtttcac cgacaaatgc
    26101 gccgcacagc agaccatgtg atatctgccc gtagcgatga taaacgtcgt cggtactttg
    26161 gccagttgct ggaagaactg gaaatctacc aggaaaagct acgcatctgg caagcgccac
    26221 cgcaggtgac ggaaccggtt catcggctgg cggggatgct ccataagtat caacatgcgt
    26281 tgaccgatag ttaagtcaaa accgacgcca aaagcgtcgg ttttttcatg gctatactta
    26341 gcgatgcacg gcagaactcg ccgcgaaacg tgacggtggc aacagatgaa tatttatgcc
    26401 tttgatttta atgagattga gagtcaggag gatttttatc gtgactttag ccaaaccttt
    26461 ggtctggcta aagataaggt acgcgatctc gactcactat gggatgtgtt aatgaacgat
    26521 gtcctgccgc taccacttga gattgaattt gttcatctgg gagagaaaac gcgtcgccgt
    26581 tttggcgcgt taatattgct gtttgatgag gcagaggaag agctggaagg gcatttgcgt
    26641 tttaatgttc gtcattagcg caaaaaaaaa gcccccgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggg cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtacacac taattattga
    27061 actaattaat aacacgctaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 tgggcgaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaact ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggcgtg
    30661 atcatttcag cgctggccga cgaagaggtg ctggtatcaa gcgtcacttc tacttccagt
    30721 ggtttgccgt tacgcagcag gccaagcttc actttcgtgc ccggctctgt agtcgcgata
    30781 cgagagcgca actcagcaaa gctattcagc ggtttgccgt tgaggctggt aataatatcg
    30841 cccgctttga cgcccgcttt cgctgagcca gaacttggca acacttcgct gacaaacgcg
    30901 ccacgctgca cgtcaaggtt gaaggctttg gcgatatcgg cactcatctc ggtgcctttg
    30961 atgcctaaca aaccgcgttt gatttcacca aagtcgataa gctgctgcgc cagtgttcgc
    31021 gccatattac tggggatggc aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt
    31081 gcagtgttga tgccaattaa ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg
    31141 cggttaatgg aagcatctgt ctggataaag ttttccagac cttcaagatt caacccgctg
    31201 cggcctaatg cggaaacaat gccagaggtg gcggtttgcc caaggccaaa tgggttaccg
    31261 accgctacgg caaaatcacc gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt
    31321 aatttgctcg ggttttgaat ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc
    31381 agttttgcat caaactcgcg cccatcattg agctgaatac tgattttctg tgcctgatta
    31441 atcacatggt tgttggtcag cacatagcct ttactggcgt tgatgatgac accggaacct
    31501 aaaccttcga agggttgtgc aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat
    31561 tcttccggga ttttctgtcc ctgactggcc gttccttcca cccgtacgct caccactgcc
    31621 ggaagcactt tttccagcat tggagccaga ctggggagag gggcctgatc ggcaacctgg
    31681 cctggaatcg acgcgacggc ctgaaatgac gccgagagag ttaacccggc acttaacgct
    31741 aatgcactca acagctgggt ttgttttttc attattcctg ctctcgtacc tgaatgataa
    31801 gaaaagagat tcaaaacagt ttgatgttat tgaattttca gacggctaac aatgagacac
    31861 cagattaaat gatagtcggg gagggagaga agaggagggc gcaatggctg cgcccgaaaa
    31921 ataaattagt cgcgcttcgc gccagtacgc agcaggccgg atgcgccttc agaatagtcg
    31981 cgaggcatct gcaccggtgc ctgatcgttg ctggcttcag actctgccag acgattacgg
    32041 aacgggtttg cttcagcaga cagttccggc agcaggctgc tggagctttt tgccatgtgc
    32101 tgatacagct ggcgatagtc gtgcgccatg gtatccagta attccgcgct gcgggcaaag
    32161 tggctaacca gctcttcgcg atactcgtcc agttcagctt tattcttttc cagttcgtac
    32221 tgcaacgcct gttgctggcg tagtttacga ttaccaaaac gcatggccac agcaccaata
    32281 atgatgccga cgactaaccc aattagcgca tattcccagg tcatgaacat ctcccgttgt
    32341 cttttgtttc cgtagggtgt tggcttcagg ctccatgcct gcggctgatt atgccactat
    32401 aaccgttaat tccacagaag tggaatcccg actgcatatc gcgtagtgta gaacggcctt
    32461 tttttcgtca accgtgaaca acggcgcacc gattattcaa ggaataacaa taagatcatg
    32521 caaagcgtta ccccaacatc gcaatacctg aaggcgctca atgaaggcag ccatcaaccc
    32581 gacgacgttc aaaaagaggc cgtcagccgc ctggaaatta tttatcaaga actcatcaat
    32641 agcaggccac cagcccccag gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt
    32701 aaacgcgaag acacaaagca tacgccagtg cgtggcttat atatgtgggg cggtgtagga
    32761 cgcgggaaaa cctggctgat ggaccttttc tatcaaagcc tgccgggaga gcggaaacag
    32821 cgcctgcact ttcaccgttt tatgctgcgg gtgcacgaag agctaactgc cttacagggg
    32881 cagaccgatc cgctggaaat tattgccgat cgctttaaag ccgaaattga cgtgctctgt
    32941 tttgacgaat tttttgtttc tgatattacc gacgccatgc tacttggcgg tctgatgaaa
    33001 gccctgttca cccgcggtat taccctggta gcgacgtcaa atattccgcc ggacgaactt
    33061 tatcgaaatg gcctgcaacg tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat
    33121 tgtgatgtaa tgaacgtgga cgctggtgtt gattatcgac tgcgtacact cactcaggcg
    33181 catctgtggc tttcgccact caacgatgaa acccgggcgc agatggataa actatggttg
    33241 gcgctggcgg gggtgaaacg agaaaattca ccgacgttag aaatcaacca tcggccattg
    33301 gcgacaatgg gcgtcgagaa ccagacgctg gcggtttctt ttactacgct gtgcgtcgac
    33361 gcccgcagtc agcatgacta tattgcgctc tcacgcctgt ttcacacagt aatgttgttt
    33421 gatgtgccag tcatgacgcg gttgatggag agcgaagcgc ggcgctttat tgcgctggtg
    33481 gatgagtttt acgagcgcca tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa
    33541 atttatcagg gtgagcggct gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag
    33601 atgcaaagcg aagagtatct gaagcgcgag catttggcgg gttaaaacct gccacaaatc
    33661 acaaaaaggg gtcgatcttt gaccccgact tctctataat cctgcgaccc cacgttacaa
    33721 gaaagttttt ttcccaaaac tttttgtgtg ctggcatagg ctattcgaag gggtaggttt
    33781 gccggacttt gtcgtgtgaa cctcaacaat tgaagacgtt tgggtgttca ccaacgtgta
    33841 actatttatt gggtaagctt ttaatgaaaa cttttacagc taaaccagaa accgtaaaac
    33901 gcgactggta tgttgttgac gcgaccggta aaactctggg ccgtctggct actgaactgg
    33961 ctcgtcgcct gcgtggtaag cacaaagcgg aatatactcc gcacgtagat accggtgatt
    34021 acatcatcgt tctgaacgct gacaaagttg ctgtaaccgg caacaagcgt actgacaaag
    34081 tgtactatca ccacaccggc cacatcggtg gtatcaaaca agcgaccttt gaagagatga
    34141 ttgctcgccg tcctgagcgt gtgattgaaa tcgcggttaa aggcatgttg ccaaaaggcc
    34201 cgctgggtcg tgctatgttc cgtaaactga aagtttacgc gggtaacgag cacaaccacg
    34261 cggcacagca accgcaagtt cttgacatct aatcgggatt ataggcaatg gctgaaaatc
    34321 aatactacgg cactggtcgc cgcaaaagtt ccgcagctcg cgttttcatc aaaccgggca
    34381 acggtaaaat cgtaatcaac caacgttctc tggaacagta cttcggtcgt gaaactgccc
    34441 gcatggtagt tcgtcagccg ctggaactgg tcgacatggt tgagaaactg gacctgtaca
    34501 tcaccgttaa aggtggtggt atctctggtc aggctggtgc gatccgtcac ggtatcaccc
    34561 gcgctctgat ggaatacgac gagtccctgc gttctgaact gcgtaaagct ggcttcgtta
    34621 ctcgtgacgc tcgtcaggtt gaacgtaaga aagtcggtct gcgtaaagca cgtcgtcgtc
    34681 cgcagttctc caaacgttaa ttggcttctg ctccggcaga aaacaatttg cgaaaaaacc
    34741 cgcttcggcg ggttttttta tggctaaatt ctgaatcagc gtaaaaaatg ggaagttgct
    34801 ttttgctgcc gtctgaaaga caggtaaaac aaaaaccaca tcgccaataa gggtctaagt
    34861 caactatttc agactaaagc gcatctcttt ttccccattt ccggcatcca ctcaccacaa
    34921 aggtcgcaaa atctggtaaa ctatcatcca attttctgcc caaatgtcgg gtattgctca
    34981 ttttttgttt gattttcgaa caaagagagt cgttccttat tgggtaacac aacttctgac
    35041 tggccacctg gtggctggta gcagtaaaaa ttctgactat acctggaggt tttcatggct
    35101 gtcgctgcca acaaacgttc ggtaatgacg ctgttttccg gtcctactga catctatagc
    35161 catcaggtcc gcattgtgct ggctgagaaa ggtgtaagtt tcgagatcga acacgtggaa
    35221 aaggacaatc cgcctcagga tctgattgac ctcaacccga atcagagcgt tccgaccctg
    35281 gtggatcgtg agctgaccct gtgggaatct cgcatcatta tggaatatct ggatgagcgt
    35341 ttcccgcatc cgccactgat gcctgtttac ccggtagctc gcggtgaaag ccgtctgtac
    35401 atgcatcgca tcgaaaaaga ctggtacacg ctgataaaca ctatcatcaa cggttcagct
    35461 tctgaagcag atgccgcacg taagcaactg cgcgaagaac tgctggcgat tgcgccggtc
    35521 ttcggtcaga agccgtactt cctgagcgat gagttcagcc tggtcgattg ctaccttgct
    35581 ccgctgctgt ggcgtctgcc gcaactgggc atcgagttca gcggcccggg tgcgaaagag
    35641 ctgaaaggct atatgacccg cgtctttgag cgtgactctt tccttgcttc tttaactgaa
    35701 gcagaacgtg aaatgcgtct gggccggagt taatctgtat ggatttgtca cagctaacac
    35761 cacgtcgtcc ctatctgctg cgtgcattct atgagtggtt gctggataac cagctcacgc
    35821 cgcacctggt ggtggatgtg acgctccctg gcgtgcaggt tcctatggaa tatgcgcgtg
    35881 acgggcaaat cgtactcaac attgcgccgc gtgctgtcgg caatctggaa ctggcgaatg
    35941 atgaggtgcg ctttaacgcg cgctttggtg gcattccgcg tcaggtttct gtgccgctgg
    36001 ctgccgtgct ggctatctac gcccgtgaaa atggcgcagg cacgatgttt gagcctgaag
    36061 ctgcctacga tgaagatacc agcatcatga atgatgaaga ggcatcggca gacaacgaaa
    36121 ccgttatgtc ggttattgat ggcgacaagc cagatcacga tgatgacact catcctgacg
    36181 atgaacctcc gcagccacca cgcggtggtc gaccggcatt acgcgttgtg aagtaataca
    36241 aaacaggccc aggcggcctg ttttgtcttt ttaatgactt aataaaatag cgtgattgtg
    36301 gcaatcatat tcaccacgaa accgactgcc atggggatcg ctgtccgctt caccaccgca
    36361 aaaggcgaaa cgcctgcaat tcctgacgta acgaccacca ccgcagtaat cggcgaaacc
    36421 gcgcgcgcta gcgtcgtggc aaaatgcatc ggcataatca ttacaaccgc tggtacatgt
    36481 agtccggctg cgatattcgg aataagactg gcaaatgaca taaacggcgc attgccagag
    36541 cccatcacaa tggcacaaat ggcaatgact agcgccataa taatcatcac gccaataccg
    36601 cccagaccag aatgctccgc acccctgata accgcatcaa cagtgccaat cgtcgttaag
    36661 cctttcgcaa atatttcacc cgcgaccacc agcgttacca cgttgacaaa ctgcgtaccc
    36721 atgccgtcaa aaaacgcctg cacatcgtcc attgtttcgc gcaagttatg cttgcggaag
    36781 aactcaacaa acatcgtcac cgtcaaactt agtaacatca ccaccaccag atgcagttct
    36841 gacttcatca gcccgacgtg ggcgaggaac agcgagccga gcatcaggat taacggcatc
    36901 acaggtaaaa tggcgtaata gagcggcggg acattatcga gagttttttg ctctgcctgt
    36961 tcgtgattga tatttttatc ttttttgtca aaagcgcgtt gcacgaaaaa gtgggagatc
    37021 gccaccgaga taatgacgca agaggcgacc ggaagctggt agtggaagaa gtatgtggca
    37081 attttcattc ccgcgacctg ggctgcaaaa atggagttcg tttccagaat cccccactca
    37141 atggacatcg tggttgcgat aaccgccacc gcagagaggc gacttactcc cagactcacc
    37201 agcgtcggaa ataaggtgac catcagcaac atacccagac cggaggcgct ggtaataaac
    37261 tgcgccatga tttggccgat gacgtaagtt gccgacagaa taatatacgg cgagcgaatg
    37321 agttttaacg ggcggcttag caagctcacc atcgcgcgac tggctcctat gcgctccatg
    37381 tagcgggcat aaccgcccac cgccataatc gacagcccca atccggcaat gcggttggac
    37441 agcatgcgca ggatttcatt ataaatgtcg acgatcaagt agccgctgct tttggcggga
    37501 tcgagtacac caccgaaccc taaccagact ccgcacatca tcaggaagac acctgcggtt
    37561 tgtagttttt caggatcaaa tagcccatcg taattaatac gatgacagat ataattatgc
    37621 cgaac
//
LOCUS       DAJTYG010000030        36669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTYG010000030 DAJTYG010000000
VERSION     DAJTYG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.030"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(155..1390)
                     /locus_tag="OO568_001632"
     CDS             complement(155..1390)
                     /locus_tag="OO568_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6641021.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1537..1613)
                     /locus_tag="OO568_001633"
     tRNA            complement(1537..1613)
                     /locus_tag="OO568_001633"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1577..1579),aa:Pro,seq:tgg)
     gene            complement(1656..1742)
                     /locus_tag="OO568_001634"
     tRNA            complement(1656..1742)
                     /locus_tag="OO568_001634"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1706..1708),aa:Leu,seq:cag)
     gene            complement(1763..1838)
                     /locus_tag="OO568_001635"
     tRNA            complement(1763..1838)
                     /locus_tag="OO568_001635"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1803..1805),aa:His,seq:gtg)
     gene            complement(1897..1973)
                     /locus_tag="OO568_001636"
     tRNA            complement(1897..1973)
                     /locus_tag="OO568_001636"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1937..1939),aa:Arg,seq:ccg)
     gene            complement(2076..3461)
                     /locus_tag="OO568_001637"
     CDS             complement(2076..3461)
                     /locus_tag="OO568_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6641022.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYITMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3652..4392)
                     /gene="rffM"
                     /locus_tag="OO568_001638"
     CDS             complement(3652..4392)
                     /gene="rffM"
                     /locus_tag="OO568_001638"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6641023.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4395..5747)
                     /gene="wzyE"
                     /locus_tag="OO568_001639"
     CDS             complement(4395..5747)
                     /gene="wzyE"
                     /locus_tag="OO568_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6641024.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5744..6823)
                     /gene="rffT"
                     /locus_tag="OO568_001640"
                     /gene_synonym="wecF"
     CDS             complement(5744..6823)
                     /gene="rffT"
                     /locus_tag="OO568_001640"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6641025.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHACEFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6820..8070)
                     /gene="wzxE"
                     /locus_tag="OO568_001641"
     CDS             complement(6820..8070)
                     /gene="wzxE"
                     /locus_tag="OO568_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6641026.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTVMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8072..9202)
                     /gene="rffA"
                     /locus_tag="OO568_001642"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8072..9202)
                     /gene="rffA"
                     /locus_tag="OO568_001642"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6641027.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAEITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9207..9881)
                     /gene="rffC"
                     /locus_tag="OO568_001643"
                     /gene_synonym="wecD"
     CDS             complement(9207..9881)
                     /gene="rffC"
                     /locus_tag="OO568_001643"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6641028.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9859..10740)
                     /gene="rfbA"
                     /locus_tag="OO568_001644"
     CDS             complement(9859..10740)
                     /gene="rfbA"
                     /locus_tag="OO568_001644"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6641029.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFDYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTDTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10759..11826)
                     /gene="rffG"
                     /locus_tag="OO568_001645"
     CDS             complement(10759..11826)
                     /gene="rffG"
                     /locus_tag="OO568_001645"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6641030.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGHYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11826..13088)
                     /gene="wecC"
                     /locus_tag="OO568_001646"
     CDS             complement(11826..13088)
                     /gene="wecC"
                     /locus_tag="OO568_001646"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6641031.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13085..14215)
                     /gene="wecB"
                     /locus_tag="OO568_001647"
     CDS             complement(13085..14215)
                     /gene="wecB"
                     /locus_tag="OO568_001647"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6641032.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14271..15317)
                     /gene="wzzE"
                     /locus_tag="OO568_001648"
     CDS             complement(14271..15317)
                     /gene="wzzE"
                     /locus_tag="OO568_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6641033.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15329..16432)
                     /gene="wecA"
                     /locus_tag="OO568_001649"
     CDS             complement(15329..16432)
                     /gene="wecA"
                     /locus_tag="OO568_001649"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6641034.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16672..17931)
                     /gene="rho"
                     /locus_tag="OO568_001650"
     CDS             complement(16672..17931)
                     /gene="rho"
                     /locus_tag="OO568_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6641035.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18016..18117)
                     /gene="rhoL"
                     /locus_tag="OO568_001651"
     CDS             complement(18016..18117)
                     /gene="rhoL"
                     /locus_tag="OO568_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6641036.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18258..18587)
                     /gene="trxA"
                     /locus_tag="OO568_001652"
     CDS             complement(18258..18587)
                     /gene="trxA"
                     /locus_tag="OO568_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6641037.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18718..19983
                     /gene="rhlB"
                     /locus_tag="OO568_001653"
     CDS             18718..19983
                     /gene="rhlB"
                     /locus_tag="OO568_001653"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6641038.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVETLEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20117..21601
                     /gene="gppA"
                     /locus_tag="OO568_001654"
     CDS             20117..21601
                     /gene="gppA"
                     /locus_tag="OO568_001654"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6641039.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21648..23669)
                     /gene="rep"
                     /locus_tag="OO568_001655"
     CDS             complement(21648..23669)
                     /gene="rep"
                     /locus_tag="OO568_001655"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6641040.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23756..24037
                     /gene="ppiC"
                     /locus_tag="OO568_001656"
     CDS             23756..24037
                     /gene="ppiC"
                     /locus_tag="OO568_001656"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6641041.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24083..25558)
                     /gene="ilvC"
                     /locus_tag="OO568_001657"
     CDS             complement(24083..25558)
                     /gene="ilvC"
                     /locus_tag="OO568_001657"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6641042.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMVDWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25708..26598
                     /gene="ilvY"
                     /locus_tag="OO568_001658"
     CDS             25708..26598
                     /gene="ilvY"
                     /locus_tag="OO568_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6641043.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26595..28139)
                     /gene="ilvA"
                     /locus_tag="OO568_001659"
     CDS             complement(26595..28139)
                     /gene="ilvA"
                     /locus_tag="OO568_001659"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6641044.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28142..29992)
                     /gene="ilvD"
                     /locus_tag="OO568_001660"
     CDS             complement(28142..29992)
                     /gene="ilvD"
                     /locus_tag="OO568_001660"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6641045.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(30057..30986)
                     /gene="ilvE"
                     /locus_tag="OO568_001661"
     CDS             complement(30057..30986)
                     /gene="ilvE"
                     /locus_tag="OO568_001661"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6641046.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31006..31269)
                     /gene="ilvM"
                     /locus_tag="OO568_001662"
     CDS             complement(31006..31269)
                     /gene="ilvM"
                     /locus_tag="OO568_001662"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6641047.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31266..32942)
                     /locus_tag="OO568_001663"
     CDS             complement(31266..32942)
                     /locus_tag="OO568_001663"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6641048.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAIAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGIPKDIQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVG
                     MAQAVPALREFLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLL
                     IAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQP
                     LNINDWQQHCAQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWA
                     AQHIAHTRPENFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGT
                     VKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHI
                     TRKDQVEAALDTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32945..32995)
                     /locus_tag="OO568_001664"
     CDS             complement(32945..32995)
                     /locus_tag="OO568_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641049.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33081..33179)
                     /gene="ilvL"
                     /locus_tag="OO568_001665"
     CDS             complement(33081..33179)
                     /gene="ilvL"
                     /locus_tag="OO568_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6641050.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33531..35051
                     /locus_tag="OO568_001666"
     CDS             33531..35051
                     /locus_tag="OO568_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCR6641051.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35076..35414)
                     /gene="maoP"
                     /locus_tag="OO568_001667"
     CDS             complement(35076..35414)
                     /gene="maoP"
                     /locus_tag="OO568_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6641052.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35533..36372
                     /gene="hdfR"
                     /locus_tag="OO568_001668"
     CDS             35533..36372
                     /gene="hdfR"
                     /locus_tag="OO568_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6641053.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLSGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36468..36543)
                     /locus_tag="OO568_001669"
     tRNA            complement(36468..36543)
                     /locus_tag="OO568_001669"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36508..36510),aa:Trp,seq:cca)
     gene            complement(36552..36628)
                     /locus_tag="OO568_001670"
     tRNA            complement(36552..36628)
                     /locus_tag="OO568_001670"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36592..36594),aa:Asp,seq:gtc)
BASE COUNT         8952 a        10041 c         9236 g         8440 t
ORIGIN      
        1 ccaccgcacg caaaggcttt gccggatacg atcacccaaa ccagtatcca cagaacgctg
       61 caatttattg aatttgcacg atcatgtagg ccggataagg cgtttacgcc gcatccggca
      121 atcaaccgca ggcggcttcc aaatggccgc ttttttactt attcaccacc atcaaatgtg
      181 cctgcataat gtcactggcc gggcgaccgt gcgccagcaa atcagccatt gctttaagat
      241 atggcggtag atggcggaaa taacgctgat acccggcaca caaataattc agccccggtt
      301 tgccgctggc atcgagcatg aagcggtgtt tcgggcagcc tccccaacat gcttttaaca
      361 cgttacaact gcgacactgc gccggtaatt gcttaaattt atcttcacca aacacctgct
      421 gttgcgggga atcgatcatt tctgcaattg tttgctgatg catattcccc agccgatatt
      481 gcggataaac atagtgatcg caggcgtaaa cgtcgccgtt gtgctcaaca atcaccgagc
      541 gcccacaggt tggctgatga tggcaaaccg cacccggcgc accgacaaaa ttggcaaacg
      601 cccattcgat attcatcacg aaaatcttgc cgacgtcgcg tttgatccag tggtcaaaaa
      661 tcgccaccag aaattcacca aattcatcgg ggcgcacaga ccattccgtc agttccccct
      721 gaatatcacc aggcgcatgt agtttcagtc catcgcggac agctgtttca tcagccaggc
      781 gctcgaccac cggaataaac tggatgaatt cgactcccgc atcgcacaaa aaatcataaa
      841 cctgtaacgg ttgctgcgcg ctggtgcggt tgacgcagac cagcacgtta tagtcgacat
      901 gatgtttttg caggagcgtc agggcacgca tcaccagttt atgcgtgggt ctgccgcctt
      961 tggtcacgcg atattgattg tggatctcag ccggaccatc cagcgataac ccaacaagaa
     1021 aattattttc tgccagaaat gcacaccatt cgtcatcgag cagcacgccg ttagtctgga
     1081 aactgttact tatcttcctg ccagcaccat atttcgcctg tagcgccacg gcacggcggt
     1141 aaaactccag cccgagtagc gttggttcgc cgccctgcca ggtaaaagcc acttcgtttt
     1201 gcagttcgct ggcggcgata tagtgacgga tatacgcttc cagcgtgtca tcgtccatat
     1261 gcgtgactgg cttttcgcgg taaagggatt gtttttcgag ataaaaacag tagtcacagt
     1321 tcagattaca atcggaaccg ctcggtttcg ccatcacatg aaaagcacgc gttggaacct
     1381 gttgcagcat ggtcgctcct tgcctgaagt catccttcaa tacgttaagg gcgaaatcat
     1441 accgtggggc aatgagttat tacgggcgtt tattgcgaag ggagggtgac gaaatgcaca
     1501 gaaaacaaaa aaccccgccg aagcggggtt caaaattggt cggcgagaga ggattcgaac
     1561 ctccgaccca ctggtcccaa accagttgcg ctaccaagct gcgctactcg ccgaaatact
     1621 gctttttgaa tttttagttc aattctttaa agtcgtggtg cgaggggggg gacttgaacc
     1681 cccacgtccg taaggacact aacacctgaa gctagcgcgt ctaccaattc cgccaccttc
     1741 gcattgtcac aacttctaat aatggggtgg ctaatgggat tcgaacccac gacaactgga
     1801 atcacaatcc agggctctac caactgagct atagccacca ctacaaatct tgttacgcgg
     1861 tattactacc accgcagctc aagcgccggg actaaatggc gcgcccgaca ggattcgaac
     1921 ctgagacctc tgcctccgga gggcagcgct ctatccagct gagctacggg cgcttagcgc
     1981 cgttgcgggg gtggataata cggacttccc acccctctgt ctagtccctt tttaaataaa
     2041 ttgctcgttt ggttacggtt tgtgcgtttt gctgcttatt cctccagttt atgcgcttta
     2101 ccgtggcgat taagcccaaa aactttataa atcgccgtca ccgccagcat aaagatgatg
     2161 ccgacaaaca gcgacatacg cgtatcttca ttaaagtaca tgccgatcaa aacgcaaatc
     2221 aggaatgcca ttgttatgta gttggcccac gggaacagga ttgagcggaa cggatggctg
     2281 gcaatcgccg ctttatgtgc acgccgaaaa cgcagctggc ttatcaatat cacaaaccat
     2341 ggcaccatcc ccggaagcac gctggcactg tagacgtaga caaacacacg ctgcggattg
     2401 ggaatgatgt agttcaggca tgagccaatt agcagaatag caatagatac tgccacaccc
     2461 gcaaccggta cgccgtgacg ggaaactttt gccattgccg ccggtaactg acggtttttc
     2521 gccagtgcgt agagcatacg tccgcaactg tacatgccgc tgttacagcc agagagcgca
     2581 gccgtcagca ccacaaagtt gataatgccc gccgctgcgg taataccgat tttggcaaaa
     2641 gtcagtacga acgggctgcc gttgctgcca atttcattcc acgggaagat ggtgacgata
     2701 acgaaaatcg cgcctacgta gaaaatcagg atccgccaca gcactttgcc tacggcactg
     2761 cgcagcgtca cctgcggatt cttcgcttca ccggcagtaa tgccaatcag ctccacgccc
     2821 tggtaggacg ccaccacaat acacagagcg gtcaggaacc ctttccagcc acccgcaaag
     2881 aaaccgccat gctctgtgag attgctaaaa ccaatcgact gcccgccatt gccaaagcca
     2941 aagaaaatca cgcccaggcc aatgacaatc atcacgataa tcgtggtgac tttgatcatc
     3001 gcgaaccaga actcgatttc gccgtacaac cgcaccgccg ccagattcgc caacgccacc
     3061 agcgccaccg cgatcaatgc gggtatccac tgcgccatct ccggggacca aaactggaca
     3121 taaacgccaa tggcggtgat ttctgagatc cccaccgcca tccacataaa ccagtaagac
     3181 caggcggtga gatagccaaa aaacgggctc atataacgat gcgcataaac ggcgaacgaa
     3241 ccggtaaccg gttcgaggaa caacatttcg cccattgaac gcatgatgaa aaaaacgaac
     3301 agcccggcga tgatataggc caacaatacg gatggcccgg cccatttcag ggtactggcg
     3361 gcccccataa acaggccgac gccaatggtg ccccccaggg cgatgagttc gatatgtcga
     3421 gcttccagcc cacgctgtag ctctggtttg ttatctgcca taaatcctcg tgttgtgttt
     3481 gcatgctttc cggtgttacc ggttatcgtt atgggtacat cgagtgttgc gaatgttttc
     3541 gtaattcagg agaaatggca aataaagcat taaaaatttg aatgctttgt gtaataaaaa
     3601 agcagacagg cgacgaagtg accactccgt cgctttacaa agagaggaaa atcagaggtt
     3661 gccggtgtag tgccagcgta aataacgcag caaacgaagc tgacgcttaa tgcggctcgg
     3721 ctgcgaaagc aggcggtaga gccactccag tcccagcgtt tgccagattt tcggtgcgcg
     3781 ttttacgtga ccggtgaaaa catcgtaagt cccgccaacg cccatataca gcgcatctgg
     3841 atgcaccaga cggcagtcgc gcatgaagat ctcctgcttt ggcgatccca tcgcaacggt
     3901 gacgatttgc gctccgctgg catgaatgcg ttcaaacagc gcctgacgct gctcgggttt
     3961 aaaataacca tcctgactgc caacgatatt cacattccat tggttgcgca gtttagcttc
     4021 agtttgcgcc agcacttcag gtttaccgcc cacaagaaat accggcgtcc cttctttgcc
     4081 tgcgcgcgcc atcagctctt cccagagatc ggcaccggca acgcgggaaa cctgcgcctg
     4141 cgggtacttt ttacgtactg aacgtacaac gctgatgcca tccgcatatt taaattcggc
     4201 agcgttaatt aactccctga cctcggcgtt atcttcaatg gtcagcattt tttcggcatt
     4261 aatggcaacc agcgttccct gcttaagctg cccgtcagca aacagataat cgagggcatg
     4321 ctgcatatca cgccaaccaa tcaactgtaa gccacgcagc gtataggtcg gtgccgtggt
     4381 gttgttattc attgttatcc ttcaacctgc gtccggagcg atgattttgt acgtttatga
     4441 atgagtccgg cgctttcaaa aagccagtac aacagttttg cgatcatcag acatgcgccg
     4501 aagaccacga taaaaaagac tacgcgtgag acaaacgaat ccagcccttc acgtgccagc
     4561 acgatcatat tgaagatcgc cccaaagcag aaactgtgca atatcgcagc cttatagcga
     4621 ttagtctcgc ggttgcccag ctcatacagc cagtcgaacc atttgatgat cagtccaacc
     4681 acaatcgccc cgagtgggat gaacaacgcg ccgcccatca ccaccagtga gcctataagc
     4741 gtaggcgaga tcgccagtcc ggagtggtta ttcagtactt cccaggtaaa gtaattggct
     4801 gagttcagca ccatactcgg gcgccccggc cacagccagg aagggataaa gacatagaaa
     4861 tcgcggacaa ttggagccag gccctggaag tcgatgttgt cgtagttctg caacagcaac
     4921 gccagattct cccacggtga aaaggtgtcg cgagtgagat agagaaacgt atagaacgct
     4981 tcatcgccgc tcacattcat tccatagcgt tttaacgcca gccagaacat gccaacaatc
     5041 cccagcacgc ccgccgccgc cagcatccac aacgaaatcc agccgcgaat aatgccaata
     5101 aacaggaaga tagcgaatgc gatgatgata ttggcgcgag tgccgccgac aatcatataa
     5161 gtcagcaagc caaaggcgac cgtgctgacg aggaaaaaca gccacgcttt gctgtcctgg
     5221 cgcagaaagt agaccaccag catcgccggg atgaaaaagt aaaagaagcg ttttaacgcc
     5281 acgccggaga cttcactgga aaagatctga ctgtaggagt taagccggaa cagcaaaaag
     5341 ccgttgtgca tgaagaagat gccgacgctt accagcgcga tacccatcag gatcacccac
     5401 gtaagattgg tctccacgcg gttcatggta aacagcggac ggcgcggtac atcagcaacg
     5461 cgtttgcgta ggcgggtttt gtaggtgaca tagtaaaccg cgtagaagca gcccgcagaa
     5521 agcaacgcct gcaacaagat ttctggcggc gcgacaccaa catcaaagcg aaataccagc
     5581 acgctggtca gcgggaagcc gaagaaaaag gtgagcaaaa acagcaatga aaagaagaca
     5641 ttgaagttaa agcgcacacg gcgaaactca aaccaggtca gcgtggcaat aaacagcgtg
     5701 cagagcagcc agaccacaaa caggccactg aattgcagca gactcatgcg acctccccgg
     5761 cggcaatcgc caacgcccgc tgccagcctt gtagatagtt agggctaaag aaggcaatgg
     5821 tgtttttatc cgccgacgcc aactggcgct gcgcttcacg cacaatatcc tcgttgagat
     5881 cgtcggtagt aaacagcact ggcaaatgct gttccgtcat atcctgccag aacggatttt
     5941 ccctgttaag cacacaagga atgcccgcct gaatcagtaa gcaaagcgta ccaatgccct
     6001 gctggcgggc aaaaataaag taaccaagat cgcactgacg aagtagcgcc agataggcgt
     6061 caaattccag tttttcgctc aaaatttgca gattttcttc gctgaataac tccagccccg
     6121 cctgacgaac ttcctcaatg tacgcttcgt tattaggcgg atatcccatc ggcaccacca
     6181 cttttaccgt atcgccaaat tgctgatgaa cggcacgtaa agcggcgaca tgctcattgc
     6241 tgcggtcgcc ggagttcccc accagaatgg tcatttttcc ttcacgttgc cgatcgttcg
     6301 ccatcgtatt gagagaaggg tccatccgcg tcgggaagta cagcagttct ccccgcacct
     6361 ttgggtgcgt tttggcaaaa aagctcaaat cgccgcgggt ggcaaataca cagccgactc
     6421 gcttttgcgc caggcgacgt agtgggtaaa aaagcttata tctcaagcca ctggaaagct
     6481 catacaggtc tgccccccag atatgccaga aaaactggct gggcttaatg ccaccactca
     6541 gcagagccag ccacagtgtg ggattgaact gaccgtggaa aaaaaatcgc tgctgacggt
     6601 tagcttttgc tttcgcgatg accgcttccg ccagcgattt tttcccaggg aaaaattgca
     6661 cagaaagcgc cggacagcta tcacttaagc cgtcgtcctt gccaacaacc ataaactcgc
     6721 acgcgtgctc gctcgtcgcg gccagcgcgt cattgaaaaa ccgcaaaacg gttcggttat
     6781 ggtgagggat atccgatccc agtacgtgaa tcagtacagt catgcccgcc tacgccagag
     6841 taaaaacacg ccacaacaaa gagaaaaata gacgatataa gttgccatat atgcctgcgc
     6901 cgcgcccagt gcaccatgcg cagggattag ccagtgggca aataccatca ataaagtgaa
     6961 ctggctgact tccgccagaa tataaaaccg cagtgacgct ttggcgatca ccagataacc
     7021 aaagacataa gcgcccactt ttaacacatc acccactaac tgccaggcaa agagatcgcg
     7081 cataacggta aatttattcg acaacagcaa ccagatagca aaatcacgca gcaaccagac
     7141 ggtaaaactc gctgccgcca ctgccggtaa gacgaatttc agcgatttaa ccacttcccg
     7201 ggtgatatcg cgcttttccg ttagccgcga caacgtgggc agcaaatata cgctgaacga
     7261 tgccgtaata aattgcaggt aggcatcgga aatactgctc accccttgcc agatccccac
     7321 ttcatcccag ctatactgcg ccgccagcag tttacgcatc atgatgtaag caacaggcaa
     7381 ggtcaccgac gtaatcaacg ccatgagcgt aaatttgctc aactgccctg ccagaccgtt
     7441 atcccagctg ggtttcagat agcttaacgg gatgacacca cgtttaatca acatgatggc
     7501 ggcaggaatt accaccagcg cgggaatcag cgccagaccc agcaacgccc cttcataacc
     7561 gcccaaacgg taactgacgt agtacgcgag aacgccaatc aagctgccga caatcagcga
     7621 taacgcatta cctgcggcat cgcgaaagcc tttcatcagc gccagtaaca ggttgcccca
     7681 ggcgatcccc atttgcacca gcgccactaa acgcaccagc ccctgatagt cggtattacc
     7741 aaacaatccc tggctgattg gcgcagctgc cagcacaaaa accagcgcca tcaacgtaga
     7801 gaaaccaagt accatcgctg atgaagtgcc gaccacgcgg cgcagctgtt gcggattatc
     7861 atggtactgg gcaacgtatt tggttacacc gttaaagatg ccagccccgg caagcacgcc
     7921 gagcacggta atcaactggc ggaaatttgc cgccagccca agccccgccg gaccaaatga
     7981 caccgccagc aacttaccga ccagtaaccc ggcaccaatc ttgaccagtg tactggccgc
     8041 cgtccacaag gacgcttttg ccaacgacat atcaggagaa gtagttcaac aaagtcgcaa
     8101 ttaccgtacg ctgattgacg ggcgacaggt tgtagaacag cggcaggcgc agcaggcgct
     8161 cgctctcttt ggtggtgtag cgatcttcac cgtggaactc accaaagtgt tcccccgcag
     8221 ggcaaccgtg cagcggaatg taatgaaaca ccgccatgat ttccgcttct ttcagaaagt
     8281 taatcaacgc gctccggtca tcaatatccc gcagtttaat gtagaacata tgcgcgttct
     8341 gcacgcagcc atcgggaatc gacggcagct cgatacgccc ggctttcgcc agaggcgcta
     8401 acgtatcgta gtagttttgc cacagcgcca gacgttgctg gttgatacga tccgctgctt
     8461 ccagttgcgc ccacaggtac gcagcttgca gatcggacat caaatagctg gagccaatat
     8521 cgcgccaggt atatttatcg acctgaccac ggaagaactg gctgcggtta gtgccctttt
     8581 cacggatgat ctcggctcgt tcgattaacg ctttatcgtt aatcagcgtc gcgccgcctt
     8641 caccacccgc cgtgtagttt ttggtttcat ggaagctaaa gcagccaata tgaccaatgg
     8701 ttcccagtgc acgccctttg taagtggaca tcacgccctg agcggcatct tccaccacaa
     8761 acaaattatg ctttttcgcc aacgccataa tggtgtccat ttcgcaggcc acacccgcgt
     8821 aatggaccgg cacgataacg cgcgttttgt cggtgatctc tgcttcaatc aacgtttcgt
     8881 cgatgttcat ggtgtccggg cgaacatcca caaagacgat tttcgcgcca cgcagcacaa
     8941 aggcattggc ggtggagaca aaggtgtagc tcggcatgat cacttcatcg ccaggctgga
     9001 tatcgagcag cagcgccgcc atctccagcg aagcggtgca ggacggcgtc agtaacactt
     9061 tggcgctgcc aaaacgttgc tccagccact gctggcagcg acgggtaaaa ccgccatcgc
     9121 cacacagttt gccgctaccc attgccgact gcatatagtc gagttcggtt cccaccaccg
     9181 gcggtgcgtt aaatggaatc atgtgatcac ctgtataacc agtacgcggt gctttctaca
     9241 ttcgcaccac tttgtatgta tcgtttaagc gcggcggtgt tgcccatttg ggtcgccacc
     9301 cgcaaagttg ttttaccgcg agcatacgcc cagtttagcg ccgtttgcat cagctcagca
     9361 cctgcaccgc gtccagccag caggccaatt cgcgcatctg tcgcattgag ttcccgtaaa
     9421 gagacatagc cgcgaatatc gccggacgcc gcacgtagaa tcagacattg atgatcaaag
     9481 gtgccgcgca cggcattttc aatccactgt gcataaaagc gaccgctggc gtcaggcgca
     9541 taccacggcg cacgaaaacg gctttgcgca aatgcggcgc tagctaactg acgtaatgcg
     9601 ggaatatcgg tctcttgtgc cacgacagca ccgctatcac tgacattgtt cacgggtagc
     9661 gccaaatcaa cttcaccttc taccagggag aatcccagct gttgcagggc atccagttcc
     9721 cccgtatttg atgccgcaat tttggcctgc acccgtgacc atggcgctaa ggcgtctgac
     9781 gtcaggagcg gtgcttcaga cgtaatgcgc acgatggcgc tgttaacacc aaagaaggcg
     9841 ttttcccagg ttagtggctc aatactggcg cggacgggca cgaagtaact ccagcagata
     9901 ttggccgtag ccagttttcg ctaatgaact ggcagcacgc ttcacaccct cgtcatcgag
     9961 ccagccgtta cgccaggcga tctcttccag gcaggcaatc ttaaagccct ggcgtttttc
    10021 tactgtctgc acaaaagtgc tggcctcaat caggctgtcg tgagtgtcgg tatccagcca
    10081 ggcaaatccg cgcccgagca gttcaacggt caggttgccc gcctcgaggt acatctggtt
    10141 gatggaggta atctccagtt caccgcgctc cgacggcttc acctgctttg cgtactccac
    10201 gactttactg tcgtagaaat aaagcccggt caccgcccag tttgacttcg gctgttttgg
    10261 cttttcttca agcgagatag cgcggaaatt gtcgtcaaat tccaccacgc caaagcgttc
    10321 cgggtccatc acctgatagt caaaaaccgt cgccccttcc gtgcgcgccg caacatgacg
    10381 cagcttcgga ctgaagccct gaccgaagaa gatgttatcg cccagcacca gacaagaagg
    10441 ttcaccatta aggaaggttt caccgatgat aaaggcctgc gccagaccgt ccgggctggg
    10501 ctgttcggca tattccagct ggataccgaa ctcactacca tcgcccagca ggcgctggaa
    10561 ataaccttta tcttccggcg tagtgatgat gagaatttcg cggataccgg ccagcatcag
    10621 caccgacagc ggatagtaaa tcattggctt atcgtaaatc ggcaacagtt gcttcgatac
    10681 gccgcgcgta atcggatgca atcgggtacc ggaaccgccc gccaggataa tacccttcat
    10741 gtttgcctcc gctgaaaatt agcctttcag acctaaacgc tcaccctgat agctgccgtc
    10801 ctgcacctgc ttccaccagc tttcattagc cagataccac tgaaccgttt tacgcattcc
    10861 actttcaaag gtttcctgcg gcagccagcc aagttcacgg gcaattttcg aagcatcaat
    10921 ggcataacgc agatcatgcc ccggacggtc agcgacaaag gtgatcaggt cacgatagtg
    10981 cgccacgccg tgcggcttgt tcgaagccag ttcttccagc agctcgcaaa tggtttccac
    11041 aacatcgaga ttcttacgct cgttgtgacc accaatatta taggtttcac cgactttccc
    11101 ggtggtcgcc acgcaataca gcgcgcgggc gtgatcttcc acatacagcc agtcacggat
    11161 ttgctgcccg ttgccatata ccggcagcgg tttacccgcc agcgcgttga ggatcatcag
    11221 cgggatcagt ttttccggaa agtggtaatg accgtagtta ttcgagcagt tggtgatcag
    11281 cgtcggcaga ccgtaggtcc gcagccaggc gcgcaccaga tggtcgctgc tggcttttga
    11341 cgcggaataa gggctgctcg gcgcatacgg cgtggtttcg gtgaagaaat catccgtcga
    11401 gtgcaggtca ccatatactt cgtcggtgga gatatgatga aaacggaacg ctgatttttt
    11461 atcttccgtc agcgtattcc agtaagcccg cgccgcttca agcaatgtat aagtcccgac
    11521 aatgttggtt tcaataaacg ctgccgggcc gtcaatagaa cggtcaacat ggctttcggc
    11581 tgccagatgc atgacacagt ctggctgatg ctcagtgaat acgcgtgcca gttctgcccg
    11641 atcgcagata tcaactttct caaaggcaaa gcgctcgctt tgcgcgaccg gtgccagcga
    11701 catcaggttt ccggcgtagg tcagcttatc gaccactacc accgcatcgc tcgtttcgtt
    11761 gatgatataa cgcaccagcg ccgagccaat aaacccggca ccacctgtta tcagaatttt
    11821 tctcatcagc gccagactcc tttggcatcg acgacatact gctgatggac attgtcgcca
    11881 ttgataactt tgaactgact atgatcgacc agcatcacca gcacgtctgc cgttgccagc
    11941 gcctcgtcaa gctgcgccag agtacaaagc ccggtcagtt ttttcggcaa ctggtggatg
    12001 ttaggctcaa caaccagcgt ttcgccgctg tgccactggg cgatcagttc agcgatttcc
    12061 atcgccgggc tttcgcgcag gtcatcaata ttcggtttaa acgccagacc aaagcaggcg
    12121 attttcagtt cactggcgcg tttatcggta gcagcaaggc agtcggcaac cgccgctttc
    12181 acctgatcga taacccagaa cggtttgtga tcgttaactt cgcgcgcggt acggataagc
    12241 cgcgcctgct gggggttctg tgccacgata aaccacggat caacagcaat gcagtgaccg
    12301 cccacgccag ggccaggctg aagaatatta acgcgagggt gacgattcgc caggcgaatc
    12361 agttcccaga cgttaatccc ctgatcggca caaatcagcg acaattcatt agcaaaagcg
    12421 atattcacat cgcggaagct gttttcggtg agcttacaca tttccgccgt ccgcgagtta
    12481 gtgacgacac actcaccttc gaggaaaatt ttgtacaatt cgctggcgcg ggccgaacaa
    12541 accggcgtca taccaccaat cacgcgatcg tttttaatca gctcgaccat aacctggccc
    12601 ggtaatacac gttccgggca gtaagcaatg ttgacgtccg cctgctcgcc cacctgctgt
    12661 gggaaagtca gatccgggcg catctctgct aaccattcag ccatcttctc ggttgacccc
    12721 accggcgagg tggattcaag gatcaccagg gcgccttttt tcagcactgg cgcaatggag
    12781 cgagcagccg attcaacgta ggtcatatct ggctcatgat cgcctttaaa cggcgtaggt
    12841 acagcaatca gccaggcatc cgcttcaact ggcgtcgtgc tcgctcgtaa aaaaccgcct
    12901 tctacggcag tttttactac gctcgccaaa tcaggctcga cgatatggat ttcgccacga
    12961 ttgatggtat caaccgcgtg ttggttgata tcgacaccaa tcacctgttt ttgccgtgag
    13021 gcaaacgctg ctgctgtagg cagcccgatg taacccagtc cgataacaga aatggtcgca
    13081 aaactcatag tgatatccga ttatttttta acgcttccag aatgcgagag catgcctgac
    13141 catcaccata cgggttatgg gcgcggctca tagcttgata ttcgttttcg tcttttaaaa
    13201 gacgcgtcac ttcctcgaca attcgctgct tatccgtgcc taccagacgc accgtacccg
    13261 ccgtcaccgc ttccggacgc tcagtggtat cgcgcatcac cagcaccggt ttccccagcg
    13321 aaggcgcttc ttcctgaatg ccgcctgagt cagtcaaaat cagccaggcg tggttcatca
    13381 gccagacaaa cggtaaatac tcctggggat cgatcagaat gacatttttc acatgcccca
    13441 gaatgcgatt gaccggctct ctgacgttcg ggttgagatg caccggatag acaatctgga
    13501 tgtcctggtg cgtggtggcg atgtctgcca gcgcgtggca gatttcttca aagccacgac
    13561 cgaaactctc acgcctatga ccggtcacca gaatcatctt tttatcgggg gcgataaacg
    13621 ggtaatttgc cgccagttct gaacgcagcg tgtcgctgct catcacctgg tcacgcaccc
    13681 ataacagtgc atcaatgact gtattaccgg taatgaagat tcggctatcc gcaacgtttt
    13741 cacgcagcaa gttttgccgg gaagtttcgg ttggagagaa gtgatacatc gccagatgcc
    13801 cggtcaatgt acggttagcc tcttccggcc acggcgaata gagatcgccc gtgcgcagac
    13861 cagcctcaac gtgaccaaca ggaatacgct gataaaacgc cgccaggctg gttgccagcg
    13921 tcgtcgtcgt atcgccgtga accagcacaa cgtctggttt gaactcggca agaataggtt
    13981 ttagcccttc cagaatccga caggttatct ctgtcaggcc ctgtcctggc tgcattatgt
    14041 tgagatcgta gtcaggtaca atggaaaaga gtttcagtac ctgatcaagc atctcccgat
    14101 gctgcgcagt gacgcaaact ttagcctcaa aaaaaggatc ttttgccaac gcatgcacca
    14161 acggagccat cttgatggct tccgggcgcg taccaaatac agtcagtact ttcacatcga
    14221 ttctcttcga ataagcggcg agcgcctttg cgctcaccgc agcagtgttg ctatttcgag
    14281 caacggcggg ttaatgcgac accagccccg atcagccccc cgacaatgcc ccacataatc
    14341 atcaggaagg cacgacgtgg gctatcgcgt tttaccggtt cttccggcgt acgcaaatag
    14401 cgataggtct gaaaacgcgg atccagggtt ggaccaacat tcagggtgtt taacatggct
    14461 cgattctgat catagtcgag atcaaaggcc ggaccgacgg cctgtaaatt ttccagtcga
    14521 gcctggagca ttggacgccc aagcaggaac atttctgaat caggtaattc ctcggcaggc
    14581 acatctgtcg cactgcgcga aatattatgc tgctcagcaa ttttcagcgc ctgctcaatg
    14641 ctgttcatcc ggcggtcgta gatggctttc gccacctctt cctgacgctt cacctgagct
    14701 ttcatctgga tggtgcgtgc cgcccatgcg cctttcagct catcattcag atggctggct
    14761 gcacgctggc tggcaaaagc aacatactga cgtaacaggt tattagcgtc aggcgcggtt
    14821 tcggcaataa gcttcacgct gtcattgacc gcgcgagtaa agtctccggg gataaactgg
    14881 atgttgttaa tcatttcatc cagcaacgcc gcatcggctt tgctgttgcc caccatccgc
    14941 tgtttgtaat agtcggtttg cagccagaac tctctgcggg tatcccacga ggccagttgc
    15001 ataacaaact ctttataggc ttcgtccatg accgatggtt gatcggcaga agccatgttt
    15061 gaacggacat ccaggttacg caaaaattgc tgctgcgagt aatatccccc cagcatattc
    15121 accgttggac gatcggtaat cgccgtcgag ctccactcct gacgagcaaa aaaagtatac
    15181 gccagcgcga ttaacgcaaa cgccagcccc atgccaataa tccatagctt cccagcccac
    15241 aaggtacgaa acaacccacg aatatccagt tcattttcag cgtcttcggc cggtttccca
    15301 ggcattggtt gtgtcatcac atcctcattt atttggttaa attggggctg ccaccacgat
    15361 ttctacgcag tctgcgtttt acgcgcttaa taaagcgagc aactttccag gcacgcttaa
    15421 tgcaatatcc atagaggaag aatgctagca aaaagagcac cagcatgacc cactccggga
    15481 caaaatgaga atattctgcc agcacgccaa tggaagcgag cagtgctgcg gcaagggtaa
    15541 tcagcacaaa cgcctgacgg gaagtaaacc cggcacgcat gatcaaatgg tgaatatgct
    15601 gacggtcagg agagaatggg ctcatgcctt tacgcaggcg acggtacata atcgccacca
    15661 tatccattag cggaatggcg attatccaca aagcggtaac cgggctgatg ggatgggttt
    15721 tgccctgggt cgtttcgagc aggatccaga taacggtaaa accaatcagc gtactgcccg
    15781 catcacccat aaagactttg tagcggcgac ccaggatacc aaggttaagc atgatgtatg
    15841 gcaggatggc ggcgatcatc gcaaagcacc agattgcgag gctggtttgc ccgtcgaacc
    15901 acaaaatcat accgattgct gcaaacgaga cgcaggacaa cccgcccagc aagccatcaa
    15961 tgccatcaac catgttgaac gcattaatgg ccgcccagac ggcaaatagc gtcaggaagt
    16021 aaccaaacgg tccgagcacc atctcccagg agccaaagat ataacccagg ctactgagat
    16081 aaagtttgcc gaacaccatc ataacaatgc caacagcggc ctgtatggtg gcacggattt
    16141 ttacgctgat atcaaaacga tcatccagcg cgccaatgaa aacaagcaca ccggcacaag
    16201 cgagatagag agatgcatgc ggaatatagt aatcgacaat tccgaacgtg aagcaaatcc
    16261 ctgcgtaaac cgaaatcccc ccaacgagag gtatcaatcc ctggtgacgt ttgcggaagt
    16321 ttggtttatc cactaaaccg actttttttg ccaccttacg ggcaaaaaac agaaacagtg
    16381 tcgtgaataa aaaaatactg atgagatcag tactcactgt cagtaaattc acaatgcatg
    16441 ctctcagaga aaattattag cagaagtata accacgaaga cctttattca gaagggaaag
    16501 tccgttccga acccaccagc cctgtgcaaa aattaaccat ttgatatgca aattgcataa
    16561 catgccagca aattccagga attttcctaa catcgccagc gcgacattaa gattacagat
    16621 ataaaagcaa aacgccacgt aaacacgtgg cgtttttggc ataagaccaa tttatgagcg
    16681 tttcatcatt tcgaagaaat cgtcattggt cttggtcatg gccagtttat taatgaggaa
    16741 ttccattgca tcgatttcgc ccatcgggtg aatgattttg cgcaggatcc acattttctg
    16801 cagttcttcc tgagtcgtga gcagctcttc tttacgggta ccggaacggt tgtagtcgat
    16861 agccgggaag acgcgttttt cagcgatctt acgagagagg tgcagttcca tgttgcctgt
    16921 acctttaaac tcttcgtaaa taacttcgtc cattttagaa ccggtatcga taagcgccgt
    16981 cgcgataatg gtcaggctgc cgccctcttc cacgttacgc gccgcaccaa agaagcgttt
    17041 cggacgatga agggcattgg cgtccacacc accggtcaac actttacctg acgccggaac
    17101 aacagtgttg taagcgcgcg ccagacgagt gatggagtcg agcagaatga taacgtcttt
    17161 cttgtgctca accaggcgtt tggccttctc gatcaccatt tccgcaacct gaacgtggcg
    17221 agatgcgggt tcgtcaaagg tagaagcaac aacttcacct tttaccagac gctgcatctc
    17281 ggttacttct tccggacgtt cgtcgatcag cagaaccatc agcacacaat ccgggtggtt
    17341 gtaagcaatg ctctgagcaa tgttctgcag cagcatagtt ttaccggctt tcggcggtgc
    17401 cacaatcaga ccacgctgac cacgaccgat aggtgatgcc agatccagta cgcgagcagt
    17461 taaatcttca gtagaaccgt taccacgttc catacgcaga cgagagtttg cgtgcagcgg
    17521 ggttaagttc tcaaagagga ttttgttgcg ggcgttttca ggtttgtcga agttaacttc
    17581 gttaactttc agcagcgcaa aatagcgttc accttctttc ggcgggcgaa tcttaccaga
    17641 gatggtatca ccagtgcgga ggttgaaacg gcggatttgg ctaggggaaa cgtagatgtc
    17701 atcaggaccg gcgaggtagg agctgtctgc ggaacggagg aaaccaaatc catcctgcaa
    17761 tatctccagt acgccatcac caaagatatc ttcgccactc tttgcgtgct gcttcaggat
    17821 ggcaaaaata atgtcctgct tacgcatgcg agccaggttt tccagcccca tattttcgcc
    17881 gagagtgatc agctcagaaa ccggcgtatt ctttaattcg gtaagattca tagtggtgtg
    17941 agttcttaaa cttggggtaa atctcgaact taatgttgtg aatggtatgg caggatcatc
    18001 catgcctgtt tacggtcatc gactcatgtc ttttcgctgc ctggtcacag gagagtacgc
    18061 agaactgaaa cgacaagacg gattgagtga cgagccagaa atctgttcac ttcgcattta
    18121 agataaaacg ggaagcgttg gaaattacaa gattcaaact taataaggta tgtttaatac
    18181 gaagtcaaca ctaagttagc atgactcacg ccgggcgtcc agtttttagc gacggggcac
    18241 ccgaacatga aattccctta cgccaggtta gcgtcgagga actctttcaa ctgaccttta
    18301 gacagtgcgc ccactttggt tgccgccact tcaccgtttt tgaacagcag cagagtcggg
    18361 ataccacgga tgccatattt cggcgcagtg ccagggtttt gatcgatgtt cagttttgca
    18421 acggtcagtt tgccctgata ttcgtcagcg atttcatcca gaatcggggc gatcattttg
    18481 cacggaccgc accactctgc ccagaaatcg acgaggatcg ccccgtccgc tttgagtaca
    18541 tccgtgtcaa aactgtcgtc agtcaggtga ataattttat cgctcatata taactccaca
    18601 ggaataagcc tggcgtgttg gtttcgttgt tggtgtaaca ttaaccaact aaaggttgac
    18661 tttatttcac cggatacgct ttcgtaaagc aatagtaagc tgatattcta ccacactatg
    18721 agcaaaacac atttaacaga acagaagttt tccgacttcg ccctgcatcc gaaggttgta
    18781 gaaacccttg aaaaaaaagg gtttcataac tgtacgccca ttcaggcact ggcccttccg
    18841 ctgacgctgg cgggtcgtga cgtagccggg caggcgcaaa ccggtaccgg gaaaacgatg
    18901 gcgtttctta cgtcaacgtt tcattatctt ctctctcatc ctgcgattgc cgatcgcaag
    18961 gtgaatcaac cgcgtgcctt aattatggca ccgacgcgtg aacttgccgt gcagatccac
    19021 gccgacgcgg aaccgctggc ggaagctacc ggcctgaagc tgggcctggc ttacggtggt
    19081 gatggctatg acaaacagct gaaagtgctg gaaagcggcg ttgacattct gattggcacc
    19141 acgggtcgtt taattgacta cgccaagcag aaccacatta acctcggtgc cattcaggta
    19201 gtggtactgg acgaagccga tcgcatgtac gatctaggct ttattaaaga tatccgctgg
    19261 ctgttccgcc gtatgccgcc tgcaaatcag cgcctcaaca tgctgttctc cgccacgctt
    19321 tcgtaccggg tacgtgaact ggcgttcgag cagatgaaca atgccgaata tattgaagtg
    19381 gaaccggaac agaaaacggg ccaccgtatt aaagaagagc ttttctaccc ttctaacgaa
    19441 gaaaaaatgc gtttgctgca aacgctgatc gaagaagagt ggccagacag agcgattatt
    19501 ttcgccaata ccaaacaccg ttgtgaagag atctggggcc acctggcggc agatggtcat
    19561 cgtgtcggtt tgttgacagg cgatgtcgcg cagaaaaaac gtctgcgtat tcttgatgaa
    19621 tttacccgtg gcgatctgga tattctggtt gccaccgacg ttgccgcgcg tggtttgcat
    19681 attccggcag tgacgcacgt ctttaactac gatttacccg atgactgtga agattacgtt
    19741 caccgtattg gtcgtacagg tcgcgcaggc gcaagcggtc actctatcag cctggcgtgt
    19801 gaagagtatg cattgaattt gcctgctatt gagacctata ttggtcactc aattccggta
    19861 agcaaataca atccggacgc attgatgacc gatctgccaa aaccgctgcg cctcacgcgc
    19921 ccgcgcacag gcaatggtcc gcgtcgtact ggcgctccgc gtaatcgtcg tcgttcaggt
    19981 taaataaaat gtcatactca aaatcattca aactgtatca agatggcaag agaatgaatc
    20041 ccgatgagct tactgtagta agtgattcgg gtgaaagaat gcagccaaca cagagacaga
    20101 ttgaaggatg aagagtatgg gttccacctc gtcgctgtat gcagccattg atctcggttc
    20161 gaatagtttt catatgctgg ttgtgcgcga ggtggctgga agcatccaga cgctgacgcg
    20221 aattaaacgc aaagtgcgtc tggctgctgg cctgaatagc gaaaatgacc tctctaatga
    20281 agcaatggag cgcggttggc aatgtctgcg cctgtttgct gaacgtctgc aagatatccc
    20341 tccctcgcaa attcgcgttg tcgctacggc gacattacga cttgccgtca atgcgggtga
    20401 ttttattgcc aaagcgcagg aaatcctcgg ttgtccggta caggtgatca gcggtgaaga
    20461 ggaagcgcgt ctgatttatc agggcgttgc tcacaccact ggtggtgccg atcagcgcct
    20521 ggtggtggat ataggcggtg ccagtactga actggtaacc ggcacgggtg cacaaaccac
    20581 ctcgttgttc agcctgtcga tgggctgcgt cacctggctg gaacgctatt ttgccgatcg
    20641 taatctgggg caagaaaatt ttgatgctgc agaaaaagcg gcacgcgaag tgttacgtcc
    20701 ggttgccgat gaattacggt atcacggctg gaaagtgtgc gttggtgctt ccggcaccgt
    20761 gcaggcgtta caggaaatca tgatggcaca ggggatggat gaacgcatta ccctggaaaa
    20821 gttgcagcaa ttgaaacagc gagccattca ttgcggtcgg ctggaggaac tggagattga
    20881 cgggctgacg ctggaacgtg cgttagtgtt cccgagtggt ctggcgatcc tgatcgccat
    20941 ttttaccgaa ctgaatattc agtgtatgac cctggcgggc ggtgcgctgc gtgaaggcct
    21001 ggtctacggc atgttgcatc tggccgtcga gcaggatatt cgcagccgta cactgcgtaa
    21061 tattcaacgc cgctttatga tcgatattga tcaggcacag cgcgtagcca aagttgcggc
    21121 taacttcttc gatcaggtgg aaaacgaatg gcatcttgaa gcaataagcc gcgatttgct
    21181 catcagcgcc tgtcagcttc atgaaatcgg cctgagcgtt gacttcaaac aagctccgca
    21241 acacgctgct tatctggtgc gtaacctgga tcttcccggt tttacccccg cacagaagaa
    21301 actgctggcg acgctactgc tcaaccagac taacccggtc gatctctcat cgctgcatca
    21361 gcaaaatgcc gtaccaccgc gcgtcgcaga acaactctgc cgtttactcc gcctggctat
    21421 catttttgcc agccgtcgcc gtgacgatct cgtgccagag atgacattac aggctaacca
    21481 tgaactgttg accttgacgc ttccgcaagg ttggctaacc caacatccgc tgggtaaaga
    21541 gattattgct caggaaaacc agtggcagag ctatgtccac tggccgctgg aagtgcatta
    21601 atgagtaagt gccggatgcg atgctgacgc atcttctccg gccttgatta tttccctcgt
    21661 tttgccgcca tcatcgcttt cagattcgcc agatggcttt gccctttctg catccgttct
    21721 tcggcactga ccactttgcg ctcctgttcc caaatcagat catcctgcgg cagctccagc
    21781 aaaaagcggc tcggctccgg gcgcaccagt tcgccgtact gacggcgttc tttacacagc
    21841 gtaaaggtca attccttctg ggcgcgggta atgccgacat aggccagccg ccgctcctca
    21901 tcgatattat cttcatcgat actgctctgg tgcggcaaaa acccttcttc cataccgacc
    21961 atgtagacat aaggaaactc cagccctttt gacgcatgga gagtcatcag ttgcacctga
    22021 tccagctctt cttcactctc accacgctcc atcatgtcgc gtaaagtaaa gcgcgtcacc
    22081 acctgggtga gcgtcatcgg ctcatccagt tcgctgcctt ccagcatctc cgtcatccag
    22141 ctaaacagtt ggttgacgtt cttcatgcgc atttcggcag ctttcgggct gggcgatgtt
    22201 tcgtacagcc aggattcata atccatgccg tggatcagat cgcgcaccgc ggcaatcggc
    22261 tcccgctccg ccagacgctg gatttctgcc aaccagtgag tgaagcgggt caatgcttca
    22321 taaccacgtc cgctaagcgt ctgactcagg cccatatcaa agctggcggt aaacatgctt
    22381 ttattgcgcg tcatcgccca ctcacccagc tttttcagcg tcgccgggcc aatctctcgc
    22441 ttcggcgtgt taacgatacg cagaaatgcg ctgtcatcgt ccgggttagt cagcacgcgc
    22501 agataagcca gcaagtcctt gatttcagga cgagagaaaa acgacgtacc accagaaatt
    22561 ttgtacggga tgcggttttg catcaggaat ttttcaaaca cccgcgactg atggttaccg
    22621 cgataaagaa tagcgtaatc tttgtactgc gttttattga cgaagtgatg cgcaatcagt
    22681 tcaccagtga cgcgctcggc ttcatgttct tcgttattcg cgcttaaaac ttttagctct
    22741 gtgccgtaac ccagttcgga gaacagacgc ttttcaaaga cgtgcgggtt attggcgatc
    22801 aggatgttcg ccgctttcaa aatacgtccg gaagagcggt agttctgctc aagcttaatc
    22861 accttcagcg ccggaaaatc ctggctcagc agcaccaggt tttgcggacg tgcgccgcgc
    22921 caggagtaga tcgactggtc atcgtcaccc accacggtaa agcgcgcgcg gctgcccacc
    22981 agcagtttca ccagttcata ctggctggtg ttggtgtcct gatattcgtc caccagcagg
    23041 tagcgaattt tgttctgcca gcgctcgcgg acttcttcat tgcgttgcag aagtagcgtc
    23101 ggcaataaaa tcagatcatc gaagtcgagc acattacagg ctttcaggtg tgcatcatac
    23161 agcccataac aatgggcaaa aatacggtcc cgctcgccaa tcgcactggc tgccgcctgg
    23221 gacggtgttt tgagatcgtt cttccagtta gagatggtcg aaatcaactg ttgcaggaga
    23281 actttgtcat cttcaatcag cccttcggtc aactctttga gcaacgccag ctgatcggta
    23341 tcgtcaaaca gcgaaaagtt cgccttcatc cccagcgccg catactcacg cttgatgata
    23401 tccagcccca gcgtgtggaa ggttgagatc atcagcccac gcgcctcttt gcgacccagc
    23461 gtctgtccga cacgctcttt catctcgcgc gccgccttat tggtaaaggt caccgccgcg
    23521 atatgtcgcg cctgataacc gcacccgcgg atcagatggg cgattttatt ggtgataaca
    23581 cgagttttac cggaacccgc acccgccagc accaggcagg ggccggtaac gaattcgaca
    23641 gcttgttgtt ggccggggtt tagacgcata ggaaaatcgc tcaatgaaag tcgggagggg
    23701 aaaataacag cgtggtagta tagccagcct catccttacc actcaaggca cgatcatggc
    23761 aaaaacagca gcagcactgc atatccttgt aaaagaagag aaactggctc tggatcttct
    23821 cgtgcagatt aagaacggtg ccgatttcgg caagctggcg aagaaacact ccatttgccc
    23881 atcaggcaaa cgcggcggtg atttaggtga attccgccag ggtcagatgg ttccggcgtt
    23941 cgataaagtt gtgttctctt gtccggtact ggagccaacc ggcccgctgc acacccagtt
    24001 cggttaccac atcattaagg tgctgtaccg taactaatag caaggccttc tccgggagaa
    24061 ggccttgaac tctgaatcgc tattaacccg caacagcaat acgtttcata tccgtcatat
    24121 agccgcgcag tttcttacct acttgctcaa tcgcatggct gcgaatcgct tcatttacat
    24181 cacgcagctg cgcgttatct accgcacctt ccggaatagc tttacccaaa tcgcccggtt
    24241 gcagttctgc cataaacggt ttcagcagcg gcacacaagc gtaagagaac agatagttac
    24301 cgtactcggc agtatcagag ataaccacgt tcatttcata cagacgctta cgggcgatgg
    24361 tgttggcaat cagcggcagt tcgtgcagtg attcatagta agcagattct tcgatgatgc
    24421 cggaatcaac catggtttcg aacgccagtt caacgcccgc tttcaccatc gcgatcatca
    24481 gtacgccttt atcgaagtac tcctgctcgc cgattttgcc ttcatactgc ggcgcggttt
    24541 cgaaggcggt tttgccggtc tcttcacgcc aggtcagcag tttcttatca tcgttggccc
    24601 agtccaccat cataccggag gagaattctc cggagatgat gtcgtccata tgtttctgga
    24661 acagcggtgc catgatctct ttcagctgtt cagacaacgc gtaagcacgc agtttcgccg
    24721 ggttagagag acggtccatc atcagtgtga taccgccctg cttcagcgct tcggtgatgg
    24781 tttcccaacc gaactgaatt agtttttctg cgtatgccgg atcggtgccc tcttccacca
    24841 gcttgtcgaa gcacagcagc gagccagcct gcaacatacc gcacaggatg gtttgctcac
    24901 ccatcaggtc agatttcact tccgcaacga aggacgattc cagcacgccc gcacggtgac
    24961 caccggttgc tgccgcccat gctttagcga tcgccatgcc ttcgcctttc ggatcgtttt
    25021 ccgggtgaac ggcaatcagc gtcggtacgc cgaatccacg tttgtactct tcacgcactt
    25081 cggtgccagg gcatttcggc gcaaccatga ctacggtgat gtctttacgg atctgctcgc
    25141 ccacttcgac gatgttgaaa ccgtgcgagt aacccagcgc cgcgccgtct ttcatcagtg
    25201 gctgtacggt gcgcactaca tcagagtgct gcttgtccgg cgtcaggtta accaccagat
    25261 ccgcctgtgg gatcagttct tcgtaagtac ccactttaaa accattttcg gtcgctttac
    25321 gccaggacgc gcgcttctcg gcaatcgctt ctttacgcag agcgtaggag atatcgagac
    25381 cagaatcacg catgttcaga ccctggttca gaccttgtgc gccacagccg acgatgacta
    25441 cttttttacc ctgaaggtag ctcgcgccat cggcgaattc atcgcgcccc ataaagcgac
    25501 atttgcccag ctgtgccagc tgctgacgca gattcagtgt attgaagtag ttagccatgg
    25561 tgattcctcg tgatgttgtg cttcttattg ttcggttcgc gtttgcgaga ttgaattcac
    25621 tatatgacag gaaatttatt gcggaaattg atatattcac aacgtcacat tgcaatttct
    25681 gcaacgtcaa cattgagggc tgcgccattg gatttacgcg atctgaaaac cttcctgcat
    25741 ctggcggaaa gccgccattt tggccgcagc gcgcgggcga tgcacgttag cccgtccacg
    25801 ctctcacggc agattcagcg cctggaagaa gatctcggcc agccgctgtt tgtgcgcgat
    25861 aaccgcacgg tgacgctgac tgaagcgggc gaagaactgc gcgttttcgc ccagcaaacg
    25921 ctgttgcagt atcagcagct gcgccacacc atcgaccagc aagggccgtt gctctatggc
    25981 gaattacata tcttctgctc ggtaaccgcc gcttacagcc atctgccgcc gattctggat
    26041 cgcttccgcg cggaacaccc gtcggtggag atcaaactca ctaccggtga tgcggcagat
    26101 gcaatggaaa aagtcgtcac aggtgaagct gatctggcga ttgccgggaa gccagagact
    26161 ctgcccggcg cggtggcgtt ttcgatgctg gagaatctgg cagtggtgtt gattgcaccc
    26221 gcattgccct gcccggtgcg taatcaggtt tctgtcgaaa agccggactg gtcaacggta
    26281 ccgtttatca tggccgatca ggggccggtg cgccgccgca ttgagttgtg gttccgacgc
    26341 aataaaatca gtaacccgat gatttacgcc acggttggcg ggcatgaagc gatggtatcg
    26401 atggtggcgc tcggctgtgg cgtggcattg ttgccggaag tggtgctgga aaacagcccc
    26461 gaaccggtgc gtaaccgcgt gatgatttta gagcgcagcg atgagaaaac gccgtttgag
    26521 ctgggcgtgt gtgcgcagaa aaagcggcta catgagccgc taattgaggc attctggaag
    26581 attttgccga accattaacc cgccaaaaag aacctgaacg ccgggttatt ggtttcgtcg
    26641 tggcaatcgt agcccagctc attcagccgg gtttcgaaat ccggttcatg gtcgccaagt
    26701 tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg gaacaaagaa
    26761 atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg tgattccggg
    26821 aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc gaccatatag
    26881 cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta gccgccgtcg
    26941 ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg cacgccgaca
    27001 aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac cgatcgcccg
    27061 ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt caccgccaac
    27121 aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag gccgtggaag
    27181 ttcacgttgg caccggaaag aatatgcgcc agccgttcgc cgcgaatgtt gtgctgggcg
    27241 atatattttt tcattcccgc cagcgccagc gcgccagagg gttccgccac cgcacgcaca
    27301 tcttcgaata aatccttcat cgccgcacag atcgcatcgc tatcgacggt gatgatgtcg
    27361 tcgagatact cctggcataa acggaaggtt tcgtcaccga tgcgttttac cgcgacacct
    27421 tcggcgaaca gtcccacgcg tggcagatca accggatgac ccgcatccag cgctgctttc
    27481 aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgcggcat cagttgtttg
    27541 atcagcaccg ctacgcccgc cgccagaccg ccgccgccga ctggcacaaa tacgcggtcg
    27601 agatgggagt cctgctggag cagttccagc gccagcgtgc cttgcccggc aatcaccatc
    27661 ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc gatcgcttta
    27721 gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa tccacgtacc
    27781 gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt cacgcctaac
    27841 cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt gatcacgccg
    27901 tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg cagcttaaag
    27961 ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag acgcgacgac
    28021 agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg cgcgcgcagc
    28081 actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga gtcagccatt
    28141 attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg gttgccaggc
    28201 tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc gtccaggctt
    28261 tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg cttacctgta
    28321 actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca atcagaccaa
    28381 tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca gaggtgccgc
    28441 cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt gatttcagga
    28501 agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct tcatagcgga
    28561 tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc gcgtcgtcct
    28621 ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg acgcccgcgg
    28681 ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg ccgccgtctt
    28741 tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca tcgagagaat
    28801 cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg cctgcgcgga
    28861 acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc agagtttgcg
    28921 gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg cgatccagtt
    28981 caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg tatttttggg
    29041 tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg atatcactca
    29101 tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc gtgttggtag
    29161 agccgcccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg ctagcaatat
    29221 tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat tcaacaatgc
    29281 gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc agcgagccgt
    29341 tgcccggctg cgacaggccc agcgcttcgg tcaggcagtt cattgagtta gcggtaaaca
    29401 tcccggagca ggagccgcag gtcggacacg cggaacgttc aacctgatcg ctctgggagt
    29461 cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagt ttgattatct
    29521 gatcggacag tttggttttc ccggcctcca tcgggccgcc ggaaacaaag atcaccggaa
    29581 tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag ttagagatgc
    29641 agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca gcgatcagtt
    29701 cgcgagatgg aagtgaataa agcatccccc cgtggcccat ggcaatccca tcatccaccg
    29761 caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg gcgaccagtt
    29821 taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag ttcacaaccg
    29881 cgataatcgg cttgccaaaa tcggcgtcgg tcattccggt ggcgcgccac agcgcacgag
    29941 cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc atactttatt
    30001 tactcccagt gtctgtctcg taaatgggac ggtgcgtgcc gtcccatttt ttgtatttat
    30061 tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa gaggccgaag
    30121 aaggcttgct gaatgcgttt ggtaaccggg ccacaacggc cttcgccaat ctgaataccg
    30181 tctacgctgc gcactggcgt gatttctgcc gccgtaccgg acataaagac ttcatccgcc
    30241 aagtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag ctctttcgcc
    30301 agtttgatga tggcatcacg ggtaataccc ggcagcgcgg aggaggtgaa cggtggggtg
    30361 aacagcacac catctttcac ttcaaacagg ttttcgcctg cgccttcaga gatataaccg
    30421 ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct acccaccagc
    30481 agggaagaga gatagttacc accggctttt gccgcagttg ggatggtgtt tggtgctgcg
    30541 cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc gcccagatac
    30601 gctccccacg ggaaagcggc gataatcaca tcggttgagt atcccgctgg cgggtttact
    30661 cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt gagattgttt
    30721 ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgtga aaccgggaag
    30781 cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg gaatacaacc
    30841 ggtcctttgt gtgagtcgta gcaacggatg ccttcaaaaa ccgaggtgcc atagtgcagc
    30901 gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc attgaaccaa
    30961 atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg cgcggatttg
    31021 ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca gtttatttaa
    31081 ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga tatttatatt
    31141 ttgtgcatcg ctggcggcag ccatattcat tgagcagacg tggaaaccac gatgacgcac
    31201 cacgcgtaaa acacgttcta aggtttccgg attgaagcga gccgatacat tgacctgatg
    31261 ttgcatcatg ataatttctc caacatttct gaattactgg cacctggcgg caccagcggc
    31321 cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc actgttcagc
    31381 atggtgtcga gtgccgcttc aacctggtct ttacgggtaa tgtgttggcc agggatgccg
    31441 aaggcgctgg ctaacatgag gaaatcgggg ttatcggtaa gggtggtttc gctgtatcgt
    31501 tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt atcgagtaag
    31561 acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac attcatcatg
    31621 aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac ttgtgcgcca
    31681 actgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat gaaattttcc
    31741 gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac atctgtggtc
    31801 acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg agcgtagata
    31861 gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc gcagtgttgc
    31921 tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt taaatcacct
    31981 tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat atccatatgg
    32041 ataacactgg cgtgtggtgc gaaggtgttc agtttgccgg tcacccggtc atcaaaacgt
    32101 gcacccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc tttggttccg
    32161 tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc cagccctttc
    32221 agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc cggaactgcc
    32281 tgcgccatcc ccacgccacc gccaacgtac agcatcggtt tttgcgcttt tgccagcatc
    32341 tggcgcgctt gctcgacttc ggcatgtggg aaagtcactt cgttttcaac ggtggtgaac
    32401 cacggttcca gatcgccgct ggctaactgg atatcttttg ggatgccgct ggctaactgg
    32461 atatcttttg ggatatcgac cagaaccgga ccaggacgac ctgagctggc aacgtcgaat
    32521 gcttcagcca tgatgcgcgg caactcttcc agagactgca ccagaaagct gtgcttggta
    32581 caggctaacg acaatcccag gacatccact tcctgaaatg cgtcggtgcc gataaacggt
    32641 gcggacacct gaccagtgat ggcaacgaca gggatggaat ctaacagtgc gtccgcaagc
    32701 ccggttatca ggttggttgc gcccggacca gacgtggcga tacatacgcc agttttaccg
    32761 gtagcacggg cataaccgat agccgctatt gccgcaccct gctcatgtcg gcacagcaag
    32821 tgctccacgc cgccgtcata caatgcatcg taaaccggca taattgcgcc acccggataa
    32881 ccgaaaacgg tgttcacacc ctgtgcccgc aacgcatgta ccacccactg tgcgccattc
    32941 atagttagtt ccccgtcctg aatcttgaga aacagaattt tgtgctgtta ttcattgtct
    33001 gctcctcggt tatgttttta aggtcaaaaa aacccccgga cctttcggtg cgggggtctt
    33061 agttcgttaa ggcttgatct ctaagccttt cctcgtccaa gtgcagcccc gcacggtggg
    33121 ataataatca ccaccacgct aatcacgacc aggctaatca ctcgtagaag ggctgtcatt
    33181 ttgtcttttc ttgcatcttg ttcgaaggaa tgcctaaaga gttaccatag attttgcgaa
    33241 tagcacaaga tatttttggg cctattttta tgatggcttt aataatttca atagaaattg
    33301 ttgtttttta aaaagaaaat agtgagtgaa aaaattcaat ttctgtataa atgttgttcc
    33361 cttcctcgta gttcatcctt tgtccgctgc atttttgcga gcatcattcc accgccagaa
    33421 caactcaact tcacttgcac aaataaacag gaatcagcca ctaaaattca cgaattagcg
    33481 ggctacagca gcgatatctt atgcacataa tggtgactca aggagggttt atgtcactgt
    33541 caattgttca tacccgcgca gccctggaag taaatgcgcc cccaatcact gttgaggtac
    33601 atatcagtaa aggtctaccc ggcttaacga tggtgggctt aacagaaaca acggtgaaag
    33661 aagctcgcga tcgcgtgcgc agcgccatta tcaatagcgg atatgaatat ccggcgaaaa
    33721 aaatcaccat caaccttgct ccagccgatc tgccaaaaga agggggacga tatgatttac
    33781 ctatcgccat tgcgttgctg gcggcctcag aacagcttac agccaataag ttagatgaat
    33841 atgaattagt cggagaacta gcgcttacag gcgctctgcg tggcgttccc ggtgcaatct
    33901 ccagtgcaac tgaagctatt aagtcgggca gaaaaattat cgtcgcgaaa gataacgaag
    33961 atgaagtggg gctaattaac ggtgaaggat gcctgatagc cgatcatctg caggctgtct
    34021 gtgcgtttct ggaaggtaag aacactctcg aacgcccgaa accaactgat gcagtatccc
    34081 gggcgctaca acatgatctc agtgatgttg tcggtcagga gcaaggaaaa cgaggactgg
    34141 aaattaccgc cgctggtggg cacaaccttt tactgattgg gccgccggga acaggtaaaa
    34201 caatgctcgc cagccgtatt aatggcctgt tgccagattt aagcaatgaa gaggcactgg
    34261 agagcgctgc gatattaagt ctggtaaatg ctgaatcagt acaaaaacaa tggcggcagc
    34321 gcccgttccg ctcacctcat cacagtgcgt cgttaactgc gatggtgggc ggtggtgcaa
    34381 ttccagggcc aggtgaaatt tcgttggcgc ataacggcgt gctttttctt gatgagctac
    34441 ctgaatttga acggcgtaca ctggatgcct tgcgagagcc gattgaatcc gggcagatcc
    34501 atctttcacg cacacgagca aaaataacct atccagcccg tttccagctt gttgcggcga
    34561 tgaatcccag ccctaccgga cattatcagg gaaaccataa ccgctgcacg ccagaacaga
    34621 cattacgtta tctcaaccgg ctctcggggc cctttctcga ccgcttcgat ctctcactgg
    34681 agatcccatt accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg
    34741 ccaccgttaa acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac
    34801 tgaatgcctg gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg
    34861 cgatgtggtt ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt
    34921 tactgaaagt tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc
    34981 atttgcagga ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac
    35041 tactgacata aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct
    35101 tcagcacctt caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta
    35161 atacgcgtca tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca
    35221 cggcatactg ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc
    35281 aactcattga aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca
    35341 ccatgacgag agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta
    35401 aagctttccg ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt
    35461 attagttacg cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt
    35521 gcggagagca ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt
    35581 cactttggtc gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc
    35641 agacaactgg aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt
    35701 ttaaccgctg ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag
    35761 gccgcccgca aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc
    35821 gcctcgttgt gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat
    35881 gcccatacag gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag
    35941 ctgcatgaac gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt
    36001 agtagtcagt tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta
    36061 aagggagatc ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca
    36121 ggtttgagcg gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag
    36181 caacagattg cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa
    36241 ggcggcctgc ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg
    36301 ctgcaaaata gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat
    36361 gaagtgtatt aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg
    36421 attatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga
    36481 gagactcgaa ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc
    36541 cctaattagg gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag
    36601 gtattctaac cgactgaact accgctccac cgaattcttt tacaaccacc ggttttatga
    36661 ccggcttac
//
LOCUS       DAJTYG010000031        36344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTYG010000031 DAJTYG010000000
VERSION     DAJTYG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.031"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            26..2293
                     /gene="adiA"
                     /locus_tag="OO568_001671"
     CDS             26..2293
                     /gene="adiA"
                     /locus_tag="OO568_001671"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6641054.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGKSEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     INEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVIHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            2618..3379
                     /gene="adiY"
                     /locus_tag="OO568_001672"
     CDS             2618..3379
                     /gene="adiY"
                     /locus_tag="OO568_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6641055.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            3516..4853
                     /gene="adiC"
                     /locus_tag="OO568_001673"
     CDS             3516..4853
                     /gene="adiC"
                     /locus_tag="OO568_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6641056.1"
                     /translation="MSSDADAHKVGLIPVILMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            4957..6600
                     /gene="eptA"
                     /locus_tag="OO568_001674"
     CDS             4957..6600
                     /gene="eptA"
                     /locus_tag="OO568_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6641057.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRTLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            6597..7265
                     /gene="basR"
                     /locus_tag="OO568_001675"
     CDS             6597..7265
                     /gene="basR"
                     /locus_tag="OO568_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6641058.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLMVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            7266..8366
                     /gene="pmrB"
                     /locus_tag="OO568_001676"
     CDS             7266..8366
                     /gene="pmrB"
                     /locus_tag="OO568_001676"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6641059.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(8342..8431)
                     /gene="pmrR"
                     /locus_tag="OO568_001677"
     CDS             complement(8342..8431)
                     /gene="pmrR"
                     /locus_tag="OO568_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6641060.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8543..10045)
                     /gene="proP"
                     /locus_tag="OO568_001678"
     CDS             complement(8543..10045)
                     /gene="proP"
                     /locus_tag="OO568_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6641061.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(10309..11187)
                     /locus_tag="OO568_001679"
     CDS             complement(10309..11187)
                     /locus_tag="OO568_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6641062.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(11184..13412)
                     /gene="crfC"
                     /locus_tag="OO568_001680"
     CDS             complement(11184..13412)
                     /gene="crfC"
                     /locus_tag="OO568_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6641063.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            13814..14149
                     /locus_tag="OO568_001681"
     CDS             13814..14149
                     /locus_tag="OO568_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6641064.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            14307..14750
                     /gene="yjdN"
                     /locus_tag="OO568_001682"
     CDS             14307..14750
                     /gene="yjdN"
                     /locus_tag="OO568_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR6641065.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            14883..15671
                     /gene="phnC"
                     /locus_tag="OO568_001683"
     CDS             14883..15671
                     /gene="phnC"
                     /locus_tag="OO568_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6641066.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            15696..16712
                     /gene="phnD"
                     /locus_tag="OO568_001684"
     CDS             15696..16712
                     /gene="phnD"
                     /locus_tag="OO568_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6641067.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            16818..17597
                     /gene="phnE"
                     /locus_tag="OO568_001685"
     CDS             16818..17597
                     /gene="phnE"
                     /locus_tag="OO568_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR6641068.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAEEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            17618..18343
                     /gene="phnF"
                     /locus_tag="OO568_001686"
     CDS             17618..18343
                     /gene="phnF"
                     /locus_tag="OO568_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR6641069.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            18344..18796
                     /gene="phnG"
                     /locus_tag="OO568_001687"
     CDS             18344..18796
                     /gene="phnG"
                     /locus_tag="OO568_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR6641070.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            18793..19377
                     /gene="phnH"
                     /locus_tag="OO568_001688"
     CDS             18793..19377
                     /gene="phnH"
                     /locus_tag="OO568_001688"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR6641071.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            19377..20441
                     /gene="phnI"
                     /locus_tag="OO568_001689"
     CDS             19377..20441
                     /gene="phnI"
                     /locus_tag="OO568_001689"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR6641072.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLVHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            20434..21279
                     /gene="phnJ"
                     /locus_tag="OO568_001690"
     CDS             20434..21279
                     /gene="phnJ"
                     /locus_tag="OO568_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR6641073.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            21276..22034
                     /gene="phnK"
                     /locus_tag="OO568_001691"
     CDS             21276..22034
                     /gene="phnK"
                     /locus_tag="OO568_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR6641074.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            22145..22825
                     /gene="phnL"
                     /locus_tag="OO568_001692"
     CDS             22145..22825
                     /gene="phnL"
                     /locus_tag="OO568_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR6641075.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            22822..23958
                     /gene="phnM"
                     /locus_tag="OO568_001693"
     CDS             22822..23958
                     /gene="phnM"
                     /locus_tag="OO568_001693"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR6641076.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLLLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     SGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTQN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            23958..24515
                     /gene="phnN"
                     /locus_tag="OO568_001694"
     CDS             23958..24515
                     /gene="phnN"
                     /locus_tag="OO568_001694"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR6641077.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQALARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            24502..24936
                     /gene="phnO"
                     /locus_tag="OO568_001695"
     CDS             24502..24936
                     /gene="phnO"
                     /locus_tag="OO568_001695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR6641078.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            24938..25696
                     /gene="phnP"
                     /locus_tag="OO568_001696"
                     /pseudo
     CDS             24938..25696
                     /gene="phnP"
                     /locus_tag="OO568_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
     gene            complement(25843..26172)
                     /locus_tag="OO568_001697"
     CDS             complement(25843..26172)
                     /locus_tag="OO568_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641079.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRCQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(26241..26873)
                     /locus_tag="OO568_001698"
     CDS             complement(26241..26873)
                     /locus_tag="OO568_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6641080.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRVAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGGCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            complement(26954..29169)
                     /locus_tag="OO568_001699"
                     /pseudo
     CDS             complement(26954..29169)
                     /locus_tag="OO568_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            29283..30803
                     /locus_tag="OO568_001700"
     CDS             29283..30803
                     /locus_tag="OO568_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6641081.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            30800..31774
                     /locus_tag="OO568_001701"
     CDS             30800..31774
                     /locus_tag="OO568_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6641082.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            31805..32746
                     /locus_tag="OO568_001702"
     CDS             31805..32746
                     /locus_tag="OO568_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6641083.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     ISYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            32801..33484
                     /locus_tag="OO568_001703"
     CDS             32801..33484
                     /locus_tag="OO568_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HCR6641084.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     VVFDLKLGWDVTAFGGNNFAAQGLMLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            33499..34359
                     /locus_tag="OO568_001704"
     CDS             33499..34359
                     /locus_tag="OO568_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HCR6641085.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            34356..35357
                     /locus_tag="OO568_001705"
     CDS             34356..35357
                     /locus_tag="OO568_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR6641086.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLLLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDVFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(35354..36060)
                     /locus_tag="OO568_001706"
                     /pseudo
     CDS             complement(35354..36060)
                     /locus_tag="OO568_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT         8452 a        10164 c         9663 g         8065 t
ORIGIN      
        1 ttccttcata accgggtaag caatgatgaa agtattaatt gttgaaagcg agtttctcca
       61 tcaagacacc tgggtcggta acgccgttga gcgtctggca gatgctttaa gccagcaaaa
      121 tgttaccgtg attaaatcca cctcctttga tgatggtttt gccattctct cttcaaacga
      181 agccattgac tgcctgatgt tcagctatca aatggaacat ccggacgaac atcaaaacgt
      241 cagacaattg atcggtaagc ttcatgagcg ccaacaaaac gtgccggtct tcctgttggg
      301 cgatcgggaa aaagccctcg ccgcaatgga ccgggatctg ctggagcttg tcgatgaatt
      361 cgcctggatt ctggaagata ccgccgactt tatcgccgga cgcgccgttg ccgcgatgac
      421 ccgctaccgc cagcagctgt tgccgccact gttcagcgcg ctgatgaaat atagtgacat
      481 ccatgaatat tcctgggcag cgccaggcca ccagggcggc gttggtttta ccaaaacacc
      541 cgccggacgt ttctaccatg actactatgg tgaaaatctg ttccgcaccg acatgggcat
      601 cgaacgaact tccctcggtt ctttgcttga ccatactggc gcatttggca aaagcgaaaa
      661 atatgccgca cgcgtatttg gtgccgatcg ctcctggtcg gtagtcgtcg gtacttccgg
      721 ctctaaccgc accatcatgc aggcttgcat gaccgataac gatgtcgtgg tcgttgaccg
      781 taactgccat aaatccatcg aacaaggttt gatgctgaca ggcgcgaaac cggtctatat
      841 ggtgccaagc cgcaaccgct acggcattat cgggccaatc tatccgcagg aaatgcaacc
      901 tgaaaccttg caaaagaaaa tcagtgaaag cccgctgacc aaagacaaag ccgggcaaaa
      961 accgtcttac tgcgtggtaa ccaactgcac ctatgacggc gtgtgttata acgctaaaga
     1021 agcgcaggat ctgctggaaa aaacctccga tcgtctgcac tttgacgaag cctggtacgg
     1081 ctatgcacgt ttcaacccga tctatgccga tcactatgcc atgcgcggcg aacctggcga
     1141 tcacaacggt cctaccgttt tcgccaccca ctccatccac aaactgctga atgcgctgtc
     1201 acaggcttct tatattcatg tacgtgaagg tcgtggggcg attaacttct cccgcttcaa
     1261 ccaggcctac atgatgcatg ccaccacctc cccgctgtat gccatctgcg catccaacga
     1321 cgtggcggtg tcgatgatgg acggcaacag cggcctgtca ctgacacagg aagtgattaa
     1381 cgaagcggtt gatttccgtc aggcgatggc gcggctatat aaagagttca ccgctgacgg
     1441 tagctggttc ttcaaaccgt ggaacaaaga agtcgtcacc gacccacaaa ccggcaaaac
     1501 ctatgacttt gctgacgcac caaccaaact gctgaccacc gttcaggact gctgggtaat
     1561 acatccgggc gaaagctggc acggcttcaa agatattccg gataactgga gtatgctcga
     1621 cccaattaaa gtcagcatcc ttgctccggg aatgggtgaa gatggtgaac tggaagaaac
     1681 cggtgttccg gcggcgctgg tcactgcctg gcttggtcgc cacggcattg tgcctacccg
     1741 caccactgac ttccaaatta tgttcctgtt ctctatgggc gtaacccgtg ggaaatgggg
     1801 gactctggtt aacacccttt gctccttcaa acgccactat gacgccaaca caccgctggc
     1861 gcaggtgatg ccggaacttg ttgaacaata tcctgacact tacgcgaaca tggggattca
     1921 cgatctgggt gacaccatgt ttgcctggct gaaagaaaac aaccctggcg cacggttgaa
     1981 cgaagcctat tccggcctgc cggtggcgga aatcaccccg cgtgaagcgt acaacgcgat
     2041 tgtcgacaac aatgtcgaac tggtatccat tgaaaatctg ccaggacgca tcgcggcaaa
     2101 ctcagttatc ccgtatccgc caggaatccc gatgctgctg tctggtgaaa acttcggcga
     2161 taaaaacagt ccgcaagtaa gttatttacg ctcgctgcaa tcctgggacc accatttccc
     2221 tggatttgaa cacgaaactg aagggactga aattattgac ggtatttacc acgttatgtg
     2281 cgtgaaagcg taaccactat tccgctgaag gcgtaattgt ttaaataaca ttacgccgcc
     2341 tggccttagg ccttttgagt atggcaacgt tttcataaaa attgctgcaa acaaaaatgt
     2401 catacttttt gcgcggcccc accccgcgct tttttttgcc tgttatttat cctgtaaaaa
     2461 aatatgtaca tgagaaatta ctataaaaat ttgtaatatt agtaaaactc gttattttta
     2521 tgcatgttta tattcatcat acaattatat aaccatttcc cggtatcgct ttgctttagc
     2581 gagaactggt cttttattcg cactcaggag tacatgtatg aggatttgca gcgaccaacc
     2641 ttgtattgtt ttattgactg aaaaagatgt ctggataagg gtgaatggga aagaacctat
     2701 tagccttaaa gctaaccata tggcgttatt aaattgtgaa aataatatta tcgacgtctc
     2761 ctctcttaac aacactttgg ttgctcatat tagtcacgac atcatcaaag attacctccg
     2821 gtttctgaat aaagatctct cgcaaatacc agtatggcaa cgtagcgcta cgcccatact
     2881 caccctgcca tgcctgacgc cagacgtctt tcgcgttgcc gcgcaacaca gcatgatgcc
     2941 cgcagaaact gagtcagaaa aggaacgaac acgcgcatta ttattcacgg tgctatcccg
     3001 ttttctcgac agtaaaaaat tcctttcatt aatgatgtat atgttacgta attgtgtaag
     3061 tgacagcgtt tatcaaatta ttgaaagcga tattcacaaa gactggaatc ttagtatggt
     3121 agccagttgt ttatgtctta gcccaagtct gttaaagaaa aagttgaaaa gcgaaaacac
     3181 cagttatagc caaataatca ccacctgccg catgcgttat gccgtaaatg aattaatgat
     3241 ggacggtaaa aatatctctc aggtatcaca gtcctgcggc tacaacagta cgtcgtactt
     3301 tatttctgtc tttaaagact tctacggtat gacgccgctg cattatgtta gtcagcacag
     3361 agaacgcact gtcgcctgat ttttaacctt aacgaagagc tatattaata acggcatcag
     3421 cgataacccg gtcgataata attcaactat cgaatgcagg cgtatgatat gacgtaattt
     3481 attgtcacga agctcgcctt cgcaggagtt taattatgtc ttcggatgct gatgctcaca
     3541 aagtgggctt aatccccgtc atcctgatgg tgtcggggaa tattatgggg tcaggtgttt
     3601 ttctgttacc tgcaaacctg gcctccactg gcgggattgc catttatgga tggttggtga
     3661 cgattatcgg tgcgctgggg ctctcgatgg tatacgccaa aatgtcgttc ctcgacccaa
     3721 gtcctggtgg ttcttacgct tacgcccgcc gctgctttgg cccgtttctc ggttatcaaa
     3781 ccaacgtcct ctactggctg gcctgctgga tcggcaatat cgccatggtg gtcattggcg
     3841 taggatattt aagttacttc ttcccgattc tgaaagatcc attggtatta accatcacct
     3901 gcgtcgtggt gctgtggatc ttcgtcctgc tgaacattgt cggtccgaaa atgatcaccc
     3961 gcgtgcaggc cgttgccact gtgctggcgc tgatccccat cgtcgggatt gccgtatttg
     4021 gctggttctg gttccgtggt gaaacctata tggcggcatg gaacgtcagc ggcctgggca
     4081 ccttcggtgc gattcaaagt acccttaacg ttacgctgtg gtcgttcatc ggtgtggaaa
     4141 gtgcctccgt tgccgcaggt gtggtgaaaa acccgaaacg caatgtccct atcgccacca
     4201 ttggtggggt attgattgcc gccgtttgct atgtgctctc caccaccgcg attatgggga
     4261 tgatccctaa tgccgcactg cgcgtttctg cctcgccatt cggtgatgcc gcacggatgg
     4321 cgttgggtga caccgctggg gccattgttt ccttctgcgc agctgcgggt tgcttaggtt
     4381 cactgggcgg ctggacgttg ctggcgggtc aaacggcgaa agccgctgcc gatgacggac
     4441 tgttcccacc gatttttgcc cgtgtaaata aagcgggtac gccagtggcg gggctgatta
     4501 tcgtcggtat tttgatgacc atcttccagc tcagcagcat ttcaccaaac gcgaccaaag
     4561 agttcggtct ggtttcttcc gtctcggtca tctttacact ggtgccatat ctttacacct
     4621 gtgcggcgtt actgctgctc ggccacggtc actttggtaa agcacgcccg gcatatctgg
     4681 ctgttactac cattgccttc ctctactgca tttgggccgt ggtggggtcc ggggcgaaag
     4741 aggttatgtg gtcatttgtc accctgatgg tcatcaccgc catgtatgcg ctgaattaca
     4801 accggctaca taaaaacccg tatcccttag atgcaccaat aagcaaagat taattcccct
     4861 taatccagca aacataaaag ccaaccttaa gaacttaagg ttggcttaat tttgctttgc
     4921 gagcatatgc gcactttgtt cgatggaaac accgtgatgt tgaagcgcct actaaaaaga
     4981 ccctctttga atttactcgc ctggctattg ttggccgctt tttatatctc tatctgcctg
     5041 aatattgcct tttttaaaca ggtgttgcag gcgctgccgc tggattcgct gcataacgta
     5101 ctggttttct tgtcgatgcc ggtcgtcgct ttcagcgtga ttaatattgt cctgacacta
     5161 agctctttct tatggcttaa tcgaacactg gcctgcctgt ttattctggt tggcgcggct
     5221 gcacaatatt tcataatgac ttacggcatc gtcatcgacc gctcgatgat tgccaatatt
     5281 attgatacca ctccagcaga aagttatgcg ctgatgacac cgcaaatgtt attaacgctg
     5341 ggattcagcg gcgtgcttgc tgcgctgatt gcctgctggc taaaaatcaa acctgccacc
     5401 tcgcgtctgc gcagtgttct tttccgtgga gccaatattc tggtttctgt actgctgatt
     5461 ttgctggtcg ccgcactgtt ttataaagac tacgcctcgt tgttccgcaa taacaaagag
     5521 ctggtgaaat ccttaagccc ctctaacagc attgttgcca gctggtcatg gtactcccat
     5581 cagcgactgg caaatctgcc gctggtgcga attggtgaag acgcgcaccg caacccgtta
     5641 atgcagaacg aaaaacgtaa aaatttgacc atcctgattg tcggcgaaac ctcgcgggcg
     5701 gagaacttct ccctcaacgg ctacccgcgt gaaactaacc cgcggctggc gaaagataac
     5761 gtggtctatt tccctaatac cgcatcttgc ggcacggcaa cggcagtttc agtaccgtgc
     5821 atgttctcgg atatgccgcg tgagcactac aaagaagagc tggcacagca ccaggaaggc
     5881 gtgctggata tcattcagcg agcgggcatc aacgtgctgt ggaatgacaa cgatggcggc
     5941 tgtaaaggtg cctgcgaccg cgtgcctcac cagaacgtca ccgcgctgaa tctgcctgat
     6001 cagtgcatca acggcgaatg ctatgacgaa gtgctgttcc acgggctgga agagtacatc
     6061 aataacctgc agggtgatgg cgtgattgtc ttacacacca tcggcagcca cggtccgacc
     6121 tattacaacc gctatccgcc gcagttcagg aaatttaccc caacctgcga caccaatgag
     6181 atccagacct gtaccaaaga gcaactggtg aacacttacg acaacacgct ggtttacgtc
     6241 gactatattg ttgataaagc gattaatctg cttaaagaac atcaggataa atttaccacc
     6301 agcctggttt atctttctga ccacggtgaa tcgttaggtg aaaatggcat ctatctgcac
     6361 ggtctgcctt atgccatcgc cccggatagc caaaaacagg tgccaatgct gctgtggctg
     6421 tcggaggatt atcaaaagcg gtatcaggtt gaccagaact gcctgcaaaa acaggcgcaa
     6481 acgcaacact attcacaaga caatttattc tccacgctat tgggattaac tggcgttgag
     6541 acgaagtatt accaggccgc ggatgatatt ctgcaaactt gcaggagagt gagtaaatga
     6601 aaattctgat tgttgaagac gatacgctgt tattgcaggg actgattctg gcggcgcaaa
     6661 ccgaaggcta cgcgtgcgat ggcgtgacaa ccgcgcggat ggcggaacaa agccttgagg
     6721 acggtcatta cagcctgatg gtactggatt tagggttacc cgacgaagat ggactgcatt
     6781 ttctcgcccg tatccggcag aaaaaataca ccctgccggt actgatcctc accgctcgcg
     6841 atacgctgac cgacaaaatc gccgggctgg atgtcggtgc cgatgactat ctggtgaagc
     6901 cttttgcgct ggaagagtta cacgcccgta tccgcgccct gctacgacgc cataataatc
     6961 agggcgaaag tgagctgatt gttggcaatc tgacgctgaa tatgggtcgc cgtcaggtat
     7021 ggatgggcgg tgaagagttg attctgacgc ccaaagaata tgctctgctg tcacggttaa
     7081 tgctcaaagc aggcagtccg gtacatcggg aaattctcta caacgacatc tataactggg
     7141 acaatgaacc ctcgaccaac accctggaag tgcatatcca caatctgcgc gacaaagtgg
     7201 gcaaagcccg tatccgcacc gtgcgcggct ttggctatat gctggtcgcg aatgaggaaa
     7261 actaattgaa tctgatgcgt tttctgcgcc gaccaatatc gctgcgccaa cggctgatat
     7321 tgaccatcgg ggccattttg ttggtgtttg agctgatcag cgtcttctgg ctatggcatg
     7381 aaagtaccga gcagattcaa ctgtttgagc aggcgctacg cgacaatcgc aacaacgatc
     7441 gccacatcat gcgtgagatc cgcgaggcgg tcgccagcct gattgtcccc ggcgtcttta
     7501 tggtcagcct gacgctattt atctgctatc aggcggtacg ccgcatcacc cgcccgctgg
     7561 cggagctgca aaaagagctg gaagcgcgca ccgccgacaa cttaacgccc attgccattc
     7621 acagcgccac cctcgaaatc gaagcggtgg tttcggcgtt gaacgatctg gtcagtcgcc
     7681 tgaccagcac gctggataac gaaaggttgt ttaccgctga cgtcgcgcac gaactgcgaa
     7741 cgccactggc gggggtgcgt ttgcatctgg aactgctggc gaaaacgcat cacattgatg
     7801 tagcaccgtt agtggcacgg cttgatcaga tgatggagag cgtctcccag ctgctgcaac
     7861 tggcgcgtgc cggacagtca ttttcttccg gtaattatca acatgtaaaa ctgctggaag
     7921 atgtcattct cccctcgtat gacgaactca gtaccatgct cgaccagcga cagcaaaccc
     7981 tgctattgcc agagagcgcc gccgacatca ccgttcaggg tgatgcaacc ctgctgcgga
     8041 tgttattgcg aaacctggta gaaaacgccc atcgttacag cccgcaaggc agcaacatta
     8101 tgattaagct gcaagaagat ggcggagcgg tcatggcagt tgaagatgaa ggaccaggta
     8161 ttgatgagag taaatgcggg gagttgagta aagcgtttgt acgtatggac agccgttatg
     8221 gcgggattgg tctggggtta agtattgtca gccgcattac acagttgcat cacgggcagt
     8281 ttttcctgca aaaccggcaa gagacttccg gcacgcgggc ctgggtacgg ctgaagaaag
     8341 atcagaacgt ggcaaaccag atataaagaa agctgctgac caccagcacg ctgaacacgg
     8401 tagttaaact ttcataaaca cggtttttca ttgcactctc ctccgttaac ctggaggaga
     8461 gtatgcgcgt cagagattaa gtgaacctta agagttcaat tggcctgata agacagcgtc
     8521 acatcaggcc atccgtttca gcttattcat caattcgcgg atgttgctgc accaggcggg
     8581 tacgtttcgc ctgcaaatcg gcaatctcgt ggtcaatatt atcgattttc tgctcgatat
     8641 tatcgtaatg ctcgacgaga atttccttcg cttcctgtat atctgacgcc gccggtgtcg
     8701 cacctttcaa cggacgattt gccgtctctt tcatggttac gccggtgatt aaaccaatca
     8761 ccgccactac catcaggtaa taggcaggca tcatcagatt ctgcgagctt tcgaccagcc
     8821 aggccgccag tgttggcgtc agaccggcaa ccagcaccga aatattaaat gccgccgcca
     8881 gcgcgctgta acggatatgc gtcgggaaca tcgctggcaa ggtagaagcc ataacgccca
     8941 taaagcagtt aaggatcacc gccagcatca gtaacccggc aaaaatcagg ccgatgacgt
     9001 tactgttaat cagaataaac gccgggatcg ccaacacaaa cagtgcaaca ctaccaagta
     9061 gcacaaacgg acgacggcca aaacggtcac tcagcaagcc catcaccggc tggacaaaca
     9121 gcataccgat cataatggcg ataataatca gcaccccgtg gtcttcggag taatgcaggt
     9181 tatgcgacaa ataactcggc atataggtca gcaacatgta gtaagtcacg ttggtggcaa
     9241 ttaccagacc aatacatgtc aacaggctgc gccagtattt agtggcaatc tctttaaacg
     9301 agactttcgg gccatcctgc aaaccttcgc ggtcgccctg ttccagttta tcaacatgct
     9361 gctggaacgc cggagtttct tccagcgcat ggcgcaggta aagcccgata atccctaacg
     9421 gcagagcaat aaagaacgga atacgccagc cccagtcgag gaagttcgct tcgccgacaa
     9481 tggtcgaaat taacaccacc acgcccgcac ccagcacaaa cccggcaata gaaccgaagt
     9541 ccagccagct gcccataaag ccacgtttac ggtcggggga gtattccgca acaaatatcg
     9601 acgccccggt atattcacca ccgaccgaga aaccttgtgc catcttacag atcaacagca
     9661 gaatcggtgc ccaaatacca atcgtgtcgt aggacggtat taagccaata cagaacgtac
     9721 tgatcgacat aatcacaata gtgatagcga ggatcttctg gcgaccatat ttatcgccca
     9781 acataccaaa gaagagtccg ccaagcggtc gaatcagaaa gggaacggag aaagtggcaa
     9841 gtgcagcaac catctgcacg ctggggtcag cccccgggaa aaaaacttta cctaatgcgt
     9901 aagcaacaaa accataaaca ccaaaatcaa accattccat tgcattaccc agtgatgctg
     9961 cggtaatggc tttacgcagt ttaccgtcat caataatggt gacatcacga agggtaatcg
    10021 gttttacttt tttccttttc agcatagctt tcctcgcaga gttgacccat ttccgcaaac
    10081 gccagaaaaa tcagcgcgac ggccgcaaaa ggggagcggg aattgcagga tgcaatctct
    10141 gtaacaagcg tagcgggttt gcttacaccc tccggttaag gaatgtacaa tcaaaccagt
    10201 tgacgcctga attgggcagt taatgacctt tttaccctac cagtgtttaa atttgtgatc
    10261 aacttcacac atggatttcg tttaggactc attgatgtaa ctgattattt acgccaaatt
    10321 tacagagaag atttcctcga ccattgcccc caccaaacgc tgcgctgaac agagtcgtgg
    10381 catccccagc gcgacagttt gccagcgttg ggtcactgac cagcaaacgg gttgtttatc
    10441 tgccgtggtc cactttccca gccaacttaa aaaatcagca tgatccccgc ccgtcggaac
    10501 ggtcgaagag gcaaaccact gatggtaata gtgacgcgtg gtcggtgccg cattgatact
    10561 ctctgccaga tgttgtgtcg ctttctcgcg caaatcaccc agcaacaaca ccaccgcttg
    10621 cgggcttccc agacggcagg catcgccaaa tgcaccccag cgcaactcct ccagaacgat
    10681 aaaacagcgc ccaggcaacg accaggcgtt atacgcccca gcacgccagc gggtaaaaat
    10741 ttgctcgctc tgatcacctg cctgcaccag taggcctcgt tgcgttaacg ccttttcctt
    10801 ccagcgacaa cgggctgaaa accactcata caaggcggca atttgctggg ggagctccgg
    10861 gaaagcagag tgctggcggt gatccatttt ttgcagcagc gccagatgct gcgcgatctg
    10921 cgtcagcgcc agatgaccgg gatccagcat cgacgccagt ttttccacca ttgtcagtcg
    10981 cagactaagc ggcgcattta acatgccgcc gttcgtccat gtctgaagct gcacacgcga
    11041 aagaagttcc tgcatcagtc gttcacgaaa ctgctgctga tgggcttgag gatggcgggc
    11101 atcatcgccc tgcgccagat ccactaaaaa gcgcggatga accgactcca gcacgcgacc
    11161 ggggccgtcc agtaacgtct tggtcaatat cgttctgccg tgaaaagtgt ttgaatatca
    11221 tcgcgtaaca gctgagcgtc ggtgtaaatc cagctgtgag tcatcacagt ctgctgcaat
    11281 tgctgctgca tcagcctgac caccgattca ttttgttgac gcagagccag actttcgcgt
    11341 aaacgcgtct gtaattctgt caaacatgaa gcgaactcag cgaaaaaagt attcatgcct
    11401 gccgtaacag attcatcgac ctgctctgcc agaactttag ccatttgttg gcaatagaga
    11461 tcgacttctg cgcttaatgc tcgttgcaac acactgtaat caaccgtttc tgtcggggat
    11521 ttctcatttc cccgtcccca gtcgggctga ttcaaccagc gcgaaaaagt ctcacgcaca
    11581 acgcctaaac gcgtgctctg ctcgtccgtt gcttcctggc gcgaaatgac ttcactgaac
    11641 agctggcgag tgttgaagtg gggaactacg ccgtgaaaaa caggaaaatg aaacccagga
    11701 cgaaaccctg actcgctcaa ttccattttg acttgttgct caatggggcg aataacatcg
    11761 gttaacaccc ggcaaagggt ggattccagc tcggcaaaac gcagcgtaaa gtcgcgactg
    11821 atggtgttct gcgccgtctg taacagtgtc tcacagcggg tacgaatctc gcttaacggc
    11881 tccgaatcat cctgaaacaa ggcggctaac tgcgcattca ccgcatcttg ctgttgacgc
    11941 agaaagtggt tggcggaggt cagggccagc tcgatttcat gtttaatctc gccgctcacc
    12001 tgcgcctgat tgagttgcaa tagctgcaaa ctttcttcga cctgatggat attttgccgc
    12061 aattgttcac aagcgacgtt taacccgtgc gcacgaaaat ccaggtattc ccgcgcctgc
    12121 tgcgcgtaat tcaacagttt atgcgcagca gatcgcagag catacaatga ggcgttagcg
    12181 taagcggcat gaagcaacgc ctgaattggc tgggcgaaca gcgaatcttc ccacaactga
    12241 tcggcagcat gacgaatatg ttcgaggtcc gccagatcgg catgacgcca gcgcctgccg
    12301 agcgcggcat gggcaaaatc ttccacccag cgttgttgct ctggcgctgg taacttaccg
    12361 ttgttggcta actcatagcg cgcccgattc gccaggtagc cccacatcga cgacaccggg
    12421 aatatctgct gtggcgtaat acagcctttc atcagcgtcc cggaaatcag tgcccgcacc
    12481 tggtcggcgt cgtcactgtt acgatcctgt tgatcgaact tattgaccag cacatacagc
    12541 ggcaccgatt gccccaccgc caaaatcgcc tcacggacct cttcatcgga gatcgatttc
    12601 agttgcgtat aatccagcac cgccagtacc gccgaggcgc gtgccagctg ctggttaagc
    12661 attttttgca gatgcggttg cccggcttca tttggcccgg gggtatccag taacgtcaac
    12721 tgaccgggat aactttccag ccccgccaga tggacaaact ccacttcaat cacgggaata
    12781 tgctcaatgg cggcgtaagc agaaaaagga aaatcgacgt ccagcgcctt cgccagtcgc
    12841 actaaatcat tcaaactttt cagacaatga aaaataggct gggcacccag ataatatttt
    12901 tcgaaagcga cgccattttc gatccgctgc ataagcgcac gcatatcttt atctatttcc
    12961 agcacatcgg tcagatgctt aatatcgcaa tcacgcaggc gctgttgtaa ttgttgaatt
    13021 aaacaatcga ttggcgcgac atgtgaaaaa tgcagtaccg gttccttttg cccgggcgta
    13081 tggcgaataa gcgtcggcag cgcagtcatt gggcgattac gattaggcag aacctccgta
    13141 ccaacaatgg cattaatggt ggttgatttc cctgctttca tggtaccgac aattgccagc
    13201 accatttcca gtcgggaaat tttacgcaac tcattattca gcatcgcgtg acgttcggcg
    13261 atattaggct gactccaggg taaagccagt tgtggcgcgt cgtctccggg tacagagaga
    13321 ggcatttttt ccagtaactg caactgttgg cgagaaagct gtaacaggcg ttcagcctcc
    13381 tgacttaact catacagggt ctgtgtgtac atagaaaatt cttccttaaa gcaaattttg
    13441 ttacttttat ttagccagat tgtttttgag ttctgttttc ggcttttata attactgcaa
    13501 gaaataattt tatatttagt gtgttgtttt ttatcagaat aaataacgtc ttctgatgcg
    13561 tttaaaacgt cagaaagata aaaatatcat gtaaataaaa aaaagaacaa gtagagcatt
    13621 aacattatct taaataataa atagaggcaa aaagattatt ttctttttgc gtttcctttc
    13681 aaatgaaaac gatcgtcgtc taaaatcagc agtacccccg acaaactcag ggattttgtg
    13741 tataattgcg gcctttttcg gcaatctgcc gttttttggc gcttttgccc tgctgacttt
    13801 tgaggaaatc cacatgtcat taccacactg cccaaaatgc aactccgaat acacttacga
    13861 agataacggc atgtacatct gcccggaatg tgcctacgaa tggaacgacg cagaacctgc
    13921 acaggaaagc gacgagctga tcgttaaaga tgctaacggc aatctgctgg ctgacggcga
    13981 cagcgttacc atcattaaag atctgaaggt gaaaggtagc tcttcgatgc tgaaaattgg
    14041 caccaaagtg aaaaacatcc gcctggttga aggcgaccat aacatcgatt gcaaaatcga
    14101 cggttttggt ccgatgaaac tgaaatctga gtttgtgaaa aagaactgat tgtattgtga
    14161 tcggtaagcc gaataaggcg ctcgcgccgc atccggcaac gatgccgact gcctgatgcg
    14221 acgcttgcgc gtcttatcag gcagcctctc atctgtataa atttcgaact acacttaact
    14281 ggcttctctt aactgaggtc accatcatgc cgttaagtcc ctacctctct tttgccggta
    14341 actgttccga cgcgattgcc tattatcaac gtacgttggg cgcggaactg ctctataaaa
    14401 tcagcttcgg cgaaatgccg aaatcagcgc aggacagcgc cgagaactgc ccttccggaa
    14461 tgcaatttcc cgataccgcc atcgctcatg ccaacgtgcg cattgccgga agcgacatca
    14521 tgatgagcga tgctataccg tcaggaaaag ccagctactc cggctttacg ctggtgctcg
    14581 attcgcaaca ggtcgaagaa ggaaaacgct ggtttgataa ccttgccgct aacggaaaaa
    14641 tcgaaatggc ctggcaggaa actttctggg cgcatggctt tggcaaagtc accgataaat
    14701 ttggcgtacc gtggatgatt aatgtcgtca aacaacaacc aacgcaataa cccgccggga
    14761 ggcccgccct cccgcactgt catcgaattc ccgttaactc ttcatctgtt agtcactttt
    14821 aattaaccaa atcgtcacaa taatccgcca cgatggagcc acttttttag ggaggctgca
    14881 tcatgcaaac gattatccgt gtcgagaagc tcgccaaaac cttcaatcag catcaagcgc
    14941 tgcatgcggt tgatctgaac attcatcaca gtgaaatggt ggctctgctt gggccgtcgg
    15001 gttccggcaa atccaccctt ttacgtcatt taagcggttt gattaccggc gataaatccg
    15061 ccggtagcca tatcgagcta ctgggccgca ctgtccagcg cgaaggccgc ctggcccgcg
    15121 atatccgcaa aagccgcgcc aacaccggct acatctttca acaattcaac ctggtgaacc
    15181 gcctgagcgt actggagaac gtgctgattg gcgcgctcgg cagcacgccg ttctggcgca
    15241 cctgttttag ctggttcacc ggcgagcaga aacagcgcgc gttacaggcg ctgacccgcg
    15301 ttggcatggt gcattttgcc catcagcgcg tttccaccct ctccggcggc cagcagcaac
    15361 gtgtggcgat tgcccgtgcg ctgatgcagc aggcgaaagt gattctggcc gatgaaccca
    15421 tcgcctcgct ggacccggaa tcagcgcgca tcgtgatgga caccctgcgc gacatcaacc
    15481 agaacgacgg catcaccgtg gtcgtcacgc tgcatcaggt ggattacgcc ctgcgctact
    15541 gcgaacgcat cgtcgccctg cgtcaggggc acgtcttcta cgacggcagc agccaactgt
    15601 ttgataacga acgttttgac catctctacc gcagcattaa ccgcatcgaa gagaacgcga
    15661 aagctgcctg atatccccat cattgaggaa aacgaatgaa cgctaagata attgcctcgc
    15721 tggccttcac cagcatgttc agcctcagca ccctgttaac cccggcacac gccgaagagc
    15781 aggaaaaggc gctgaatttc ggcattattt caacggaatc acagcaaaac ctgaaaccgc
    15841 aatggacgcc gtttttacag gatatggaga agaaactggg cgtgaaggtc aacgccttct
    15901 ttgccccgga ctacgcgggc attatccagg ggatgcgctt caataaagtg gatattgcct
    15961 ggtacggcaa cctgtcggcg atggaagcgg tggatcgcgc caatggccag gtcttcgccc
    16021 agacggtcgc ggcggatgga tcgccgggtt actggagcgt gttgatcgtc aacaaagaca
    16081 gcccgatcaa caacctgaac gatctgctgg cgaagcggaa agatctcacc ttcggcaatg
    16141 gcgatcctaa ctccacctct ggcttcctcg tccccggtta ctacgtcttc gctaaaaaca
    16201 atatctccgc cagcgacttc aagcgcaccg tcaacgccgg gcatgaaacc aacgcgctgg
    16261 ccgtcgccaa caagcaggtg gatgttgcca ccaacaacac cgaaaacctc gacaagctga
    16321 aaacctccgc gccggagaag cttaaagagc tgaaggtgat ctggaaatcg ccgctgatcc
    16381 taggcgatcc gatcgtctgg cgcaagaatc tgtccgaaac taccaaagac aagatctacg
    16441 acttctttat gaactacgga aaaacgccgg aagagaaagc ggtgctggaa cgcctgggct
    16501 gggcgccgtt ccgcgcttcc agcgacctgc aactggtgcc gattcgccag ctcgcactgt
    16561 ttaaagagat acaaagcgtg aaaggcaata aagggctgaa tgagcaggac aagctggcga
    16621 aaaccaccga gattcaggcg cagctggatg acctggaccg cctgaacaac gcgttaagcg
    16681 cgatgagttc ggtgagtaaa gcggtgcagt aaatcgtagg tcggataaga cgccccggcg
    16741 tcgcatccga caatgtgcag gcgttgatgc cggatgcggt gcaagcacct tatccggcct
    16801 acagaccgga gccaaacatg caaaccatca ccatcgcccc acccaagcgt agctggttct
    16861 cgcttctgag ctgggccgtt gttctcgccg tgctggtcgt ctcgtggcag ggcgcggaaa
    16921 tggccccgct tacgctgatt aaagacggcg gcaacatggc aaccttcgct gccgacttct
    16981 tcccgcccga tttcagccag tggcaggatt acctcaccga aatggccgtc acgctgcaaa
    17041 tcgccgtctg gggcaccgcg ctggcagtgg ttctctccat ccccttcggt ctgatgagcg
    17101 ccgaaaactt ggtgccgtgg tgggtttacc agcccgttcg ccgcctgatg gacgcctgcc
    17161 gcgccattaa cgaaatggtc ttcgccatgc tgttcgtggt cgccgtcggc ctcggcccgt
    17221 tcgctggcgt gctggcgctg tttatccaca ccaccggcgt gctctccaag ctgctttccg
    17281 aagcggagga agcgatcgaa cccggcccgg tggaaggcat tcgcgccacc ggcgccaaca
    17341 aactcgaaga gatcctttac ggcgtgctgc cgcaggtgat gccgctgctg atctcctact
    17401 ccctctatcg cttcgaatcc aacgtccgct cggcgaccgt cgtcggcatg gtcggcgcgg
    17461 gcgggatcgg cgtcaccctg tgggaagcga ttcgcggttt ccagttccag caaacctgcg
    17521 ccctgatggt gcttatcatc gtcacggtca gcctgctgga tttcctctct caacggttgc
    17581 gtaagcactt tatctgataa gcgaggcatt gatatctatg cacttgtcta cacatccgac
    17641 cagctaccca acacgctatc aggagatagc cgcaaaactt gagcaggagc ttcgtcaaca
    17701 ctaccgctgc ggcgactatc ttcccgccga gcagcaactg gcggcgcgct ttgaggtgaa
    17761 ccgccacacc ctgcgccgcg ccatcgacca actggtggaa aaaggctggg tacagcgccg
    17821 tcagggcgtc ggcgtgctgg tgctgatgcg cccgttcgat tacccgctca acgcccaggc
    17881 gcgttttagc cagaacctgc tggaccaggg cagccatccc accagcgaaa aactgctgtc
    17941 ggtattgcgc cccgcgtccg gtcacgttgc tgacgcgctg gggattaccg agggcgagaa
    18001 cgttatccat ttgcgcaccc tgcgccgggt caacggcgtc gcgctctgtt taatcgacca
    18061 ctactttgcg gacctcaccc tttggccgac gctgcaacgc ttcgacagcg gttcgctgca
    18121 cgattttctg cgcgagcaaa ccggaattgc gctgcgccgc agccagacgc ggatcagcgc
    18181 ccgccgcgcc caggccaaag agtgccagcg ccttgaaatc ccgaatatgt cgccgctgct
    18241 gtgcgtgcgc acccttaacc accgtgacgg tgaaagcagc ccggcggagt actccgtcag
    18301 cctgacgcgc gccgacatga ttgaattcac tatggagcac tgaatgcacg cagataccgc
    18361 gacccgccag cactggatgt ccgtactggc gcacagccaa ccggctgaac tggcagcccg
    18421 cctgaacgcg ctgaacatca ccgccgacta tgaagtgatc cgcaccgccg aaaccggcct
    18481 ggtacagatt caggcgcgaa tgggcggcac cggcgaacgc ttttttgccg gcgacgccac
    18541 gctgacccgc gccgccgtac gcctgactga cggcaccctc ggctacagct gggtgctggg
    18601 gcgtgataaa cagcacaccg aacgctgcgc gctgattgat gcgctgatgc agcaatctcg
    18661 ccactttcaa aacttatcag aaacccttat tgccccgctg gacgctgacc gtatggcacg
    18721 cattgccgca cgccaggccg aagtgaacgc cagccgggtc gacttcttta cgatggttcg
    18781 cggagacaac gcatgaccct ggaaaccgct tttatgcttc ccgtgcagga tgcccagcac
    18841 agttttcgtc gcctgttaaa ggccataagc gagccgggcg tgattgtcgc cctgcatcag
    18901 ctcaagcgcg gctggcagcc gctgaatatc gccaccacca gcgtgctgct gacgctggct
    18961 gataacgaca cgccggtgtg gcttgccgcg ccattgagta acgatatcgt caatcagagc
    19021 ctgcgttttc ataccaacgc gccgctggtc agccagccgg aacaggcgac cttcgcggtg
    19081 acggatgacg gaatttccag caaacagctc aacgcccttt ccaccggcac cgccgttgcg
    19141 ccggaagcgg gcgcgacgct gattttacag gtcgccagcc tgagcggcgg gcgcatgttg
    19201 cgtctcaccg gcgcgggtat tgccgaagaa cgaatgatcg ctccgcagct gccggagtgc
    19261 attctgcacg aactcaccga gcgcccgcac ccgttcccgc tcggcatcga cctgatcctg
    19321 acctgcggcg aacgcctgct ggctattccg cgaaccactc atgtggaggt gtgctgatgt
    19381 acgttgccgt gaaagggggc gaaaaggcga tcgacgccgc ccacgccctg caagagagcc
    19441 gacgccgggg cgataccgat ttgcccgaac tgagcgtcgc ccaaattgaa cagcagctta
    19501 atctcgcggt ggatcgcgtg atgaccgaag gcggcgttgc cgaccgcgaa ctggcggcgc
    19561 tggcgctgaa acaggccagc ggcgataacg ttgaagcaat tttcctgctg cgcgcctacc
    19621 gcaccacgct ggcgaaactg gcggtaagcg agccgctcga caccaccgag atgcgtctcg
    19681 aacggcgtat ttcggcggtt tataaagaca ttcccggcgg ccagctgctt ggcccaacct
    19741 acgactacac ccatcgcctg ctcgatttca ccctgctggc aaacggtgaa gcgccgacgc
    19801 tgaccaccgc cgacagcgaa caacagccgt cgccgcacgt tttcagcctg ctggcgcgtc
    19861 aggggctggc gaagtttgaa gaagatagcg gcgcacagcc ggatgacatc acccgcacgc
    19921 cgccggttta cccctgctca cgctcctccc gcctgcaaca attaatgcgc ggggacgaag
    19981 gctatttgct ggcgctggcc tactccaccc agcgtggtta cggacgcaat cacccgttcg
    20041 cgggcgagat ccgcagtggt tacatcgacg tgtcgattgt gccggaagag ctgggatttg
    20101 cggtaaacgt cggcgaactg ctgatgaccg agtgtgaaat ggtcaacggt tttatcgacc
    20161 cgccggatga gccgccgcac ttcacgcgcg gctacgggct ggtattcggc atgagcgagc
    20221 gcaaagcgat ggcaatggcg ctggttgacc gtgcgttgca agccccggaa tacggcgagc
    20281 acgcgacagg cccggcgcag gatgaagagt tcgtgctggt gcatgccgac aacgtcgaag
    20341 ccgcaggctt tgtctcgcac ctcaaactcc cccactacgt cgatttccaa gccgaactgg
    20401 agctactcaa acgtctgcaa caggagcaga accatggcta atctgagcgg ctacaacttt
    20461 gcctacctcg acgagcagac caaacgcatg atccgccgcg ccatcttaaa agcggtggct
    20521 atccccggtt atcaggtgcc gtttggcggg cgcgagatgc cgatgcccta cggctgggga
    20581 accggcggca ttcagctcac cgccagcgtg attggcgaaa gcgacgtgtt gaaggtgatt
    20641 gaccagggcg cagacgacac cactaacgcc gtgtcgattc gcaacttctt caagcgcgtg
    20701 accggggtga acaccactga acgtacggac gatgcgacgc ttatccagac gcgccatcgc
    20761 atccccgaaa cgccgctgac cgaagatcag atagttattt tccaggtgcc gatccccgaa
    20821 ccgctgcgct ttatcgagcc gcgcgaaacg gaaacccgca ctatgcacgc gctggaggag
    20881 tacggcgtga tgcaggtgaa actgtatgaa gatatcgccc gcttcggtca tatcgccacc
    20941 acctatgctt atccggtgaa ggtgaacggg cgctacgtga tggacccgtc gccgatcccg
    21001 aaattcgata acccgaaaat ggacatgatg cccgccctgc agctgttcgg cgcagggcgc
    21061 gagaagcgca tctatgcggt gccgccgttt acccgcgtgg aaagtctcga tttcgacgat
    21121 cacccgttca ccgttcagca gtgggatgag ccatgcgcca tctgcggatc gacccacagc
    21181 tatctcgacg aagtggtgct ggatgacgcc ggaaaccgca tgtttgtctg ctccgatacc
    21241 gattattgcc gccaacagag cgaggcaaaa aaccaatgaa tcaaccgtta ctttcggtca
    21301 ataacctgac ccacctttac gcgccgggca aaggctttag cgatgtctct tttgatttat
    21361 ggccggggga agtgctgggc atcgtcgggg aatccggctc cgggaagacc acgctgctga
    21421 aatcaatctc cgcgcgcctg acgccgcagc agggggagat tcgctacgag aatcgttcgt
    21481 tgtatgcgat gagcgaggcc gaccgccgtc gcctgctgcg caccgaatgg ggcgtggtgc
    21541 atcagcatcc actcgacggc ctgcgtcgcc aggtctcggc aggcggcaat atcggcgagc
    21601 ggctgatggc gaccggggcc cgtcattacg gcgacatccg cgccaccgcg cagaagtggc
    21661 tggaagaggt ggagattccc gccaaccgga tcgacgacct gccgaccacc ttttccggcg
    21721 gcatgcagca acgtttgcag attgcccgca acctggtgac gcatccgaag ctggtgttta
    21781 tggacgagcc aaccggtggg ctggatgtgt cggtgcaggc acgcctgctc gatctactgc
    21841 gcggcctggt ggtggagctg aacctggcgg tggtgattgt cacccatgat ttaggcgtcg
    21901 cccgcctgct agcggaccgt ttgctggtga tgaagcaggg gcaagtagtg gagagtgggt
    21961 taaccgaccg cgtgctcgac gacccgcacc atccgtatac ccagctgctg gtgtcatcgg
    22021 ttttgcagaa ttgagccggt gccggatgcg gcgtaaacgc cttatctggc ctacaaatgc
    22081 gctccccgta ggtcggataa gacgcatcag cgtcgcatcc gacacccgaa ccacgaggcg
    22141 aaaaatgatt aacgtacaaa atgtcagtaa aaccttcatc ctgcaccagc aaaacggcgt
    22201 gcgcctgccc gtcctcaacc gcgcctcgct caccgtcaac gcgggcgaat gcgtggtgct
    22261 ccacggccac tccggcagcg gcaaatcaac cctgctgcgc tcgctgtacg ccaactatct
    22321 gcccgatgaa gggcaaatcc agatcaaaca cggtgacgag tgggtagacc tggtcaccgc
    22381 gccagcgcgc aaagtggtgg agatccgcaa aaccaccgtc ggctgggtga gccagtttct
    22441 gcgcgtcatc ccgcgtattt cagcactgga agtggtgatg cagccgctgc tcgataccgg
    22501 cgtaccgcgt gaagcctgcg ccgctaaagc cgcgcgtctg ctcacccgcc tgaacgtgcc
    22561 ggaacgcctg tggcacctgg cgccatcaac cttctccggc ggcgaacagc agcgcgtcaa
    22621 catcgcccgc ggctttatcg tcgactaccc gattctgctg ctcgacgaac ctaccgcctc
    22681 gctggacgcc aaaaacagcg ccgcggtggt ggaactgatc cgcgaagcca aagctcgtgg
    22741 cgcagccatt gtcggcatct tccatgacga agccgtacgt aatgacgttg ccgaccgcct
    22801 gcacccaatg gaagcctctt catgattatc aataacgtca aattggtgct ggaaaacgaa
    22861 gtggtacacg gttcactgga ggtgcaggac ggcgaaatcc gcgcctttgc cgaaagccag
    22921 agccgcctgc cggaggcgat ggacggcgaa ggcggctggc tgctgccggg gctgattgag
    22981 ctgcataccg ataatctgga taaattcttc accccgcgcc cgaaagtcga ctggcccgcc
    23041 cattcggcga tgagcagcca cgacgcgctg atggtggcaa gcggcatcac caccgtactg
    23101 gacgccgtgg cgattggtga tgtgcgcgac ggcggcgatc ggttggagaa tctggagaag
    23161 atgatcaacg ccatcgaaga gacgcagaaa cgcggcgtca accgcgccga gcaccgtctg
    23221 ctcctgcgct gcgaactgcc gcatcacacc acgctgccgc tgtttgaaaa actggtgcag
    23281 cgcgagccgg tgacgctggt gtcgctgatg gaccactcgc cgggccagcg ccagttcgcc
    23341 aaccgcgaga agtatcgcga atattatcag ggcaaatact cgctcaccga tgcgcagatg
    23401 cagcagtacg aagaagagca actggcgctt gccgcacgct ggtcgcagcc gaatcgcgaa
    23461 tccatcgccg ccctgtgccg cgcgcgacaa attgcgctcg ctagccatga tgacgccacc
    23521 cacgcccacg ttgccgaatc ccacctgctt ggcagcgtga tcgccgaatt tcccaccacg
    23581 tttgaagcgg cggaagcctc gcgcaagcat ggcatgaacg tactgatggg cgcgccgaat
    23641 atcgtgcgca gcggttcgca ctccggcaac gtggcggcca gtgaactggc gcagcttggc
    23701 ctgctggata tcctctcttc cgactactac cccgccagcc tgctggatgc ggcatttcgc
    23761 gtcgccgatg accagagcaa ccgctttacg ctgccgcagg cggtgaagtt agtgacccaa
    23821 aatccggcgc aggcgcttaa tcttcaggat cgcggggtga ttggcgaggg caaacgcgcg
    23881 gacctggtgc tggcacatcg caaggacaat catattcata tcgaccacgt ctggcgtcag
    23941 ggtaaaaggg tgttctgatg atgggaaaac tgatttggtt aatggggccg tccggctccg
    24001 ggaaagacag cctgctggcg gaaatccgcc tgcgggaaca aactcagtta ctggtggcgc
    24061 atcgctacat tacgcgcgac gccagcgccg gaagtgaaaa ccatatcgcc ctgagcgagc
    24121 aggagttttt tacccgcgcg gggcaaaatc tgttggcctt aagctggcac gctaacagtc
    24181 tgtattatgg cgtcggcgtt gagattgatc tctggctgca cgccggattc gacgtgctgg
    24241 tcaacggctc acgcgcccat ctgccgcagg cgctggcgcg ctatcaatcg gcgctgctgc
    24301 ccatttgctt acaggtttcg ccggagatcc tgcgccagcg cctcgaaaac cgtggtcgtg
    24361 aaaatgccag tgaaattaac gcccgactag cacgcgctgc ccgctatact cctcaggatt
    24421 gtcttacgct caataatgac ggcagcctgc gccagtcggt cgacaagctg ctgacgctga
    24481 ttcatcagaa ggagaaacac catgcctgct tgtgagcttc gcccggctac gcagtacgac
    24541 accgacgcag tttacgcgct gatttgtgag ctaaaacagg cggagtttga ccaccatgcg
    24601 tttcgcgtgg gctttaacgc caacctgcgc gacccaaaca tgcgctacca tctggcgctg
    24661 ctggatggcg aagttgtcag catgatcggc ctgcatttgc agtttcatct gcatcatgtc
    24721 aactggatcg gcgaaattca ggaactggtg gtgatgccgc aggcgcgcgg tctgaacgtc
    24781 ggcagtaaat tactggcatg ggcagaagaa gaagcccgcc aggccggggc cgaaatgacc
    24841 gcgctttcga ccaacgtgaa acgccacgac gcgcaccgtt tctatctgcg agaaggctac
    24901 gagcagagtc acttccgctt caccaaggtg ctgtaacatg agcctgaccc tcacgctcac
    24961 cggcactggc ggcgcacagg gcgttccggc atggggctgc gagtgcgcgg cctgcgaccg
    25021 agcgcagcgc tcaccgcagt atcgccgcca gccatgcagc ggcgtagtga agtttaacga
    25081 cgcaatcacc ctgatcgacg ccgggctgca cgatctcgcc gatcgctggt cgcccggatc
    25141 gttccagcag tttttgctga cgcattatca tatggatcac gcccaggggc tgtttccgct
    25201 gcgctggggc gtgggcgatc cgatcccgat ctacggcccg ccggatgacc agggttgcga
    25261 cgatctgttt aaacatccgg gcctgcttga tttcagccac acagtggaac cgtttgtggt
    25321 gtttgatttg taggggttgc aggtcacgcc gctgccgctt aaccactcaa aactgacctt
    25381 cggttatctg ctggaaacgg cacatagccg ggtggcgtgg ctatctgaca ccgctggcct
    25441 gccggaaaaa acgctgaaat ttttacgcaa taatcagcca caggtaatgg tgattgattg
    25501 cagccacccg ccgcgcgcgg atgcgccgcg taatcactgt gatttaaata ccgtgcttgc
    25561 gctgaatcag gttatccgct cgccacgggt gattctgacc catatcagcc accagtttga
    25621 tgcgtggctg atagaaaacg cactgccgtc ggggtttgag gcggggtttg atgggatgga
    25681 gattggggtg gcgtgatgag agggaatgtg cgcgctggtc ccctcaccct aaccctctcc
    25741 ccagaggggc gaggggagcg attgtgctcg atattgaata ttgcgctggt tttctccctc
    25801 tccccattgg ggtgaggggc gatgcctgct ccatacccaa cctcatcgcc catactcatc
    25861 ttccattctc cgctcttcat cttctagctg ccgacgctcc tgatcaagct ggcgctggcg
    25921 atcgtccagc tgcctgcggc gatcttcaaa ctggcagcgg cggtcgtcat attgtctgcg
    25981 ccgatcgtcg ctcacttcac gctgccagcc gtcgtcgcgc gaatcttcat agtctcgccc
    26041 acggtcagag ttataagcgt cattaatcgc ctgctgaata ttaccaatag tatcgtcgat
    26101 aatatcggcc tgggccggaa cggtggacag cgtgagcagg gtgaaaagaa aaagcgtgga
    26161 gtaaggtttc ataagccagt ctctgcgatg gactggctta acgttagttg agtgtgaatg
    26221 accggagtag cggagtaatc tcaaattcgg taaggcgcag actcttccat tagctggtta
    26281 gctcgctgtt tattttccgc caccaataca atgccatgac atttttgatc cggtgaaata
    26341 ctccagatac gctcttccgg aatgcagccg ccatcgtaga tcactttcgt gccatcgtag
    26401 ggccaaaatg ccccttgttc agtgtctaat acaaacacgg aaatctgatc ggactcgcgc
    26461 gcgatcttct gccagagcat cttcccgcca tcatattgat caaaatcgct gattagcgtg
    26521 ataccgcatt gcaggattag cgcttcatac agccgagttg ccagaccctt atcgcgctca
    26581 cctggagaga cacaaatacc ttccacctgt accagatccg agtcttccca ccagcgttca
    26641 acggcaatga tatcgctgcc gaactctttt ttacgctgca gatcgagagc ggcaagtacc
    26701 cgatgacgcg gcacgcctgc aaaaacttca atgtccgctt caatgatggc cactcttggc
    26761 gtctggttgt cataaattac ctgtaacaga cgtccagaaa gctcgatctc ttccaccacg
    26821 cgaaaagaac gagcaaccgt catagggcta taggtggttg gcatcacgat catatgtaaa
    26881 tcacttcaat tcagaacaga attgctcaca atagcagcac gtttgcttcg agcctcttga
    26941 taaattctga aaatcatccc tcaatttgcc ccagcacctg cgccagctgc gcgcgggtaa
    27001 agggtttgcg cagcagcgcc acgtccggca gcgcggggtt atggctgggg cgtaaatcct
    27061 gaccgctgat gagtagcagc gtcaggtgcg ggtaaagttt gcgggccgca ttgaccactt
    27121 ctgcgccgct catgccgcca gcaggtccag cgcctgttcg ccgctgctcg cttccagtgt
    27181 caaatagccc agcaggtgca actgctcgca gatggtctgg cgcaccgcgg cttcatcttc
    27241 cagcactaac accagtttat cgccattaac agctgtctgt tctaccgccg ctggcgatag
    27301 gtttttcgcc gccgtcagcg cgcggggaag ctgtaatctg accgttgtcc cctgccccgg
    27361 cgcgctttca atttccacgc gcccgccgga ctggcgcacg aaaccataga ccatcgacaa
    27421 gcccagcccg ctgccgctcc ccgtctgttt ggtggtaaag aacggctcga acacctggct
    27481 tttcaccgcc tgcgacatac cgctgccatg atcgatgacc tccagtgcca ccatgtcctg
    27541 acgctgccca ctgctgcggg taacacgctg attccaggta cgaattttga taattcccgt
    27601 ctgcccttcc atcgcgtcgc gggcgttcat caccaggttg atgatggcgt tttccagttg
    27661 gctgacgtca atccacgcgg accacgccgg agactgcgct tcaatctcaa tcgtcagcgt
    27721 cgccggtaag gagtggcgca ttaattcgct taactcttcc agcagtgttt ttagttcaac
    27781 ggcttgcgga tgtaaggact gcttgcggga aaatgccaac aatcgctggg tcagtagcgc
    27841 gccacgttcg gcggcttgct gcgcccgctg tattcgcggc gcgtcgggtg agttcgtgtt
    27901 caccagttcc aggctaccaa taattaccgc cagcagatta ttaaaatcat gggccagccc
    27961 gcccgtcagt tgcctgaccg cttttaactt ctggctgtgc aatagcgcct cttccagccc
    28021 ctgccgctcg gtttgggcaa tctccatctg cgtggttttc tctttcaaca gccgtgacgt
    28081 tcgttcaagt gatgcggtat tgcgggcaaa aacgttaaag gcgcgcgcca gttcacccaa
    28141 ctcatcgcgc cgctgtaacg ccggaaaagt gacgtccgct tcgccgtgcg ccaggcggct
    28201 catcgcgcgg gaaatcgccg tcaggttaga gccgagattg cgatagatat accaacctgc
    28261 aaacgcggtg atcaccaccg ctaacagggc gaaaatcatg atcagcctct cgccggagcg
    28321 caaatcctgg taactttgct ccacccgctg accagagagc gtcgccacct gttcggcgta
    28381 acggttgatg tcgccattaa ggatcgccac cagcgctttg atgtgaaaca tataccagct
    28441 gattgccaga tcgctttgct ccagagccgc cgaaagtggc gcgagtttac gcagttcacg
    28501 attaaaatca gcgagtagcg tattaaccaa tggcgtatcg ctgtgagttg gcaaggcaga
    28561 catcactccg accagttgat gaataatcgc ctgcggcgcg ggggtggcaa tggcggcaac
    28621 aatcagtcgg ttcatctcgt taagcaaaat attatcttgc gcgtgagtga ctttttgcag
    28681 atgccgcaga taacttaagt tttgatacag cgaacttaac agcgcgttac gctccagatg
    28741 ccgcctttgc ccgcgttcca gtattccgcc gacgctggtt tgcagttcgt tgctacgcgt
    28801 gatgatgcgc gtcaccagcc cggcttcctg ctgcgctaaa ggcgcgttcg ccagttgtgc
    28861 aagcgagctt tttaatgccc gctgtgtctc ctgcaaccgc tcggcttcgc ctttatattc
    28921 cagtgctccc accacctgcg acaatcgcac tgccgccgtt gccacgtttg ccgtgtcgcg
    28981 cgccagattc aggctgccgg tcatatcatc tacagtttgc tgctgcacca gctcttgcat
    29041 ctggctggca tgacgaaagc ccattaccgc cacgccgctg accatcaacg tcaccgccac
    29101 caccagcaaa ttcaaaagca gcaatcgccc gcgcgcgctg gcaaaaaagt gacgtccggg
    29161 gtgcgccata tcacgctccg tgaagaaaaa tcgcaatatg acaaatatga acggcttctg
    29221 acattttgca tttactttcc tgtgatggag tgctgatatc gacaacctcg caggagtcca
    29281 ccatgaacag cacgccggtt cttgagatgc gcaatattgc caaagccttc ggcaaatttt
    29341 atgcgctcaa aggggtggat ttgacggtct accctggcga gatccatgcc ctgatggggg
    29401 aaaacggcgc gggaaaaagc acgctgatga aggtgctggc gggggcgtat accgctacca
    29461 gcggcgagat cctgatagac ggcaagccct ttcacattcg cacgccaaaa gatgccttaa
    29521 gcgccggtat tacgctgatt tatcaggaga tgcagctggc acccaatttg acggtggcag
    29581 aaaatatttt cctcggcagc gagctttccc acggcgggct ggtgcagcga aaagagatgc
    29641 tggtgcaggc gcaaagagtg atcgaccgcc tcggcgcgca gtttaacgcc agcgataagg
    29701 tcatgacgct gaccattgcc gaacaacagc aggtcgaaat cgcccgcgcg ctacatcgca
    29761 acagccgcat tctggtgatg gacgaaccca ccgctgccct ctcctcccgt gaaactcacc
    29821 gtctttttga actgattatg cggctgcgcg atgaagggat ggcgattatc tacatcagcc
    29881 accgtatggc ggaagtgtat gagctttccg atcgcgtcag cgtgctgcgc gacgggcaat
    29941 acgttggcag cctgacccgc gataacctca atgccgggga gctggtgcgg atgatggtcg
    30001 gcaggccact gagcgatctg ttcaataaag agcgcgatat cccgctcggt aaagcccgcc
    30061 tgaatgttca ccacctgacc gacggcggca aagtccagcc gagctgcctg caggtgcttt
    30121 ccggcgaaat tgttggcctc gccgggctgg tgggagccgg acgttccgaa ctggcgcagt
    30181 taattttcgg cgtgcggaaa gcgacaggcg gaatgattga agtcgatggc gaaccggtgg
    30241 tgatccactc cccgcgcgaa gccatcgatc ttggcattgg ttttctcacc gaaaaccgca
    30301 aagaacaagg cttattcctt gaaatggcgg cagtcgaaaa catcactatg gcaaccctgg
    30361 agcgcgatgc cagctgggga atgctcaatc gcaaaaaagc gcaaaccatt tccgatgacg
    30421 ccattaagtt gctcaacatt cgcgtgcctc atgcccaggt acgcgcgggc gggctttccg
    30481 gcggcaatca gcaaaaactg ttgatctccc gctgggtggc gattggcccg cgcattttac
    30541 tgctcgatga acccacccgc ggcgtggacg ttggcgcaaa aagtgagatc taccggatca
    30601 tgaacgagat ggcgcgcaag ggcgtggcga tcctgatgat ctccagcgaa ctgccggaga
    30661 tagtgggaat gagcgatcgc gtccatgtga tgcgcgaagg cagcattgcc ggtgagctaa
    30721 atggcaaaaa catcacccag gaaaacatta tgaccttagc gactggcgtc aacgacgccc
    30781 attcccaggc ggtgacctca tgacgacctc tgcaaaaacc tctgccaaaa aaatgctgat
    30841 gagcgatctg atgcaaacgg tcggcatttt gcccattttg atcctgatcg tagcggtatt
    30901 tggctttatc gcgccaaact tttttaccga aagcaacctg ctaaatatta cccgccaggc
    30961 gtcgatcaac attgtgctgg cggcggggat gaccttcatc attttgaccg gtgggatcga
    31021 tctttctgta ggttcaatcc tcggcaccac ggcggtggcg gcaatggttg tctcccttag
    31081 cccggagttc gccatgttgt ctatcccggc ggcgctgatg ctcggcctgc tgctggggct
    31141 gttcaacggc gcgctggtgg cctttgccgg attgccgccg tttatcgtca cgcttggcac
    31201 ctatacggcg ctgcgcgggg cggcgtatct gctggctgat ggcaccacgg ttatcaactc
    31261 caacattaat ttcgagtgga taggcaataa ctatctcggg ccggtcccgt ggctggtggt
    31321 gattgcgctg gcggtgatcg tgctgtgctg gtttattctg cgtcgcacca cgcttggcgt
    31381 tcatatctac gccgtcggtg gcaatatgca ggcggcgcgt cttaccggca ttaaggtctg
    31441 gctggtgctg ctgtttgttt atggcatgag cggcctgctt tcagggcttg gcgggattat
    31501 gagcacctcg cggctgtaca gcgccaacgg caacttaggt atgggttacg aactggatgc
    31561 cattgcggcg gtgatcctcg gcggcaccag ttttgtcggc ggcatcggca ccatcaccgg
    31621 cacgctggtg ggggcgctaa ttatcgccac gctcaacaat ggcatgacct taatgggcgt
    31681 ctcttacttc tggcagctgg tgatcaaagg ggcggtgatc atcattgcgg tgcttatcga
    31741 caaataccgt acccgacatc atcaaagtgc ataacaacaa taccctacac atgaggaata
    31801 cagcatgaga ttgaagccct tagtgaccgc gctctgtgca ggcgcgctgc tggccgcagc
    31861 accgttcgcc caggcgaaag atctgaaatc gataggcgta acggtaggtg acctggcgaa
    31921 cccgttcttc gtacagatca ccaaaggcgc ggagctggaa gcacgcaaac tggcgggcga
    31981 taacgtcaaa gtgacgctgg tttccagcgg ctacgatctg ggtcagcagg tctcgcagat
    32041 agacaacttt atcgccgcta acgtcgatat gatcatcctt aacgctgccg attcaaaagg
    32101 gatcggcccg gcggtgaaac gggcaaaaga cgccgggatc gtggtggtgg cggttgacgt
    32161 ggcggcagaa ggtgccgatg ccaccatcac ttccgataac acccaagcag gcgaaatggc
    32221 ctgtaaatac atcaccgatc gcctgaaagg caaaggcaac gtggtgatca tcaacggacc
    32281 gccggtgtcc gccgtgcaaa accgcgtcga aggctgccag accgaattta aacgccatcc
    32341 tgacatcaag gtgctctctg acaacaagaa cgccaaaggc agtcgtgagg gcgggctgga
    32401 ggtgatgacc gggctgctgg cggcaaatcc gaagatcgac ggcgtatttg cgattaacga
    32461 tccgacagcg atcggtgccg atctggcagc aaaacaggcg caacgtaacg agttctttat
    32521 tgtcggcgtg gatggaagcc cggacgccga agaggcgctc aagcgtgaga actcgttgtt
    32581 tgtcgctact ccggcgcaag atccacaggt gatggcagcg aaagcggttg agatcagtta
    32641 tgacattttg cagggcaaac ctgcgccgaa agagccggtg ctgatcccgg tgacgatgat
    32701 cgataaaaac aacgtcggca attacaaagg ctggacggtg aaataagtcc acacgccggg
    32761 cggcgatgct gcccggccac cgctaaggag aacgctttcg atgaaacgct ccgctataaa
    32821 caacattctc ggccatacac ggcaattctt ttcgcaacat gacgtacatc tgccgccctt
    32881 tgccagtttc tctcctgctc aatggcagca gctcgacacc gccgcgtggg aggtggtttt
    32941 tgatctcaaa ctcggctggg atgttaccgc ttttggtggt aacaactttg cagcacaggg
    33001 gctgatgctg tttaccctgc gtaacggctc agcaaaagga atgccgtatg tgaaatgtta
    33061 cgccgagaag atcatgcatg tgcgcgatgc gcaggtcacg ccaatgcatt ttcactggcg
    33121 taagcgtgag gacatcatca atcgcggcgg cggcaatttg attgttgaac tgtggaatgc
    33181 cgatagcaac gagcaaacag ttgataccga cgttacggtg gtgatcgacg gctgccgcca
    33241 gactcatgtc gcaggcagtc agttacgcct gtcccccggc gaaagtatct gcctgccgcc
    33301 cggcctgtat cacagctttt gggcggaaac cggttttggc gatgttctgg ttggcgaagt
    33361 ctcttctgtc aatgacgacg accacgataa ccacttttta caaccgctgg accgctacaa
    33421 cctgattgac gaagacgaac cggcccagct ggtgttgtgt aacgaatacc gccagttcct
    33481 ctgaataagg ataacgttat gccgttgatt tctctcgctg aagggctggc acatgcccgc
    33541 gaacatcact atgcgttagg tgcgtttaat gtgctggact cacacttttt acgcgccttg
    33601 tttgctgctg ccagacaaga acgttcgccc tttattatca acattgccga agtacatttt
    33661 aaatatatct cgctggattc cctggtcgag acagtgaaat ttgaagcggc gcgtcaagcg
    33721 attccggtgg tattaaatct cgatcacggg ctgcattttg aatcggtggc gcaagcgttg
    33781 cggctgggat tcagctcggt aatgtttgat ggctccacgc tggggtatga agagaatatc
    33841 cgccagacgc gggaagtggt gaaaatgtgc catgccgttg gcgtatcggt agaagctgaa
    33901 ctgggcgcgg tgggtggtga cgaaggcggt gcgctttacg gtcatgccga tgaatctctg
    33961 tttaccgatc cgcagctggc gcgtgatttt gtcgaccgta ccggcattga cgcgctggcc
    34021 gtggcgattg gcaatgcgca cggcaaatat cgcggcgaac cgaaactcga tttcccgcgc
    34081 ctcgacgcta tccgccagca ggcggctatc ccgctggtat tacacggcgg ttcagggatt
    34141 agagatgccg atttccgccg cgccatcgaa ctgggtatcc acaaaatcaa tttctacacc
    34201 ggcatgtcac aagcggcgct ggctgccgtt gaggcccgaa tggcgcaccg tcagccgatg
    34261 tatgacgagt ttgccgagtt actactagca gtggaagccg ccatcagcga tacggttgca
    34321 caacagatgc gtattttcgg cagtgcgggg caggcatgat ggaacgtaaa gggattatcg
    34381 ccgcaggcaa tatgctggtt gaccacgtcc accagattat ccagtggcca gagcgcggct
    34441 ggctggcgga gattacccac agcgaacgct ccaccggcgg cgcgccgctt aacgtcctgc
    34501 tgacactggc gaaaatgcgc accgggttgc tgttacaggc ggtcgggcta atcggtcagg
    34561 acagcgacgg cgattacatt atggcgatgc ttgagcaata tcacgtcaat cgtcagcatg
    34621 tgcagcgcac tacctttgcc ccgacgtcaa tgtcgcaggt aatgactgat ccaagcgggc
    34681 agcggacgtt tttccattcg ccaggtgcaa acagactttt ggatcttccg gcgttcgatc
    34741 agctcgataa caccatgaag attttccatc tcggctattt actgttgctc gacagcctgg
    34801 atatgccgga cgatgtttac ggcacccgta gcgcacgcct gctggcgcaa atgcgtgata
    34861 ctggctttga aacctcactc gacctggtct cccgcaaggg cgatccgcgt tatcagccgc
    34921 tggtgttgcc cgcgttgcgc catctggatt acctggtaat taacgaactg gaggccgggg
    34981 agtttagcgg gctggaaatt cggttaccca acggcgaacc gcatatcgcg catattgctg
    35041 ccgccgcgcg tgaactgtta gatgcgggcg tgcgccagcg tgtggtgatc cactgcccgg
    35101 aaggggcgtg gggagagacc ccggaacagt gcggcgtgtg gatcccttcg caaaagttag
    35161 aacaacggga aattatcggc agcgtcggtg cgggcgatgt attttgtgcc ggatttttat
    35221 atggctgcca tgaacgctgg acgcttaccg agagcatcaa actggcacac gcctgcgcgc
    35281 gagcaagcct gttatgcgcc aatgccatcg acggggcgaa aacgctggaa gagctgcaaa
    35341 cagaaatgtc ggattaatgc acgtcggcag cgaagacata ccccagaccg cgcagagttt
    35401 taatcaacat cggctgatgc gggttgagtt cgattttgcg tcgtaagcgc atgattaata
    35461 cgtcgatggt gcgatcaaac acctccacgc tgtcgctatg agtgaacgcc agcagctgtt
    35521 cgcggcttaa tacccgacgg gcattttgag ccagggccag taacaagcca tattcgccct
    35581 gagtcagctc gacatgttgt tgtagcggac tatgcagttg gcagcgaatg gtatcgagat
    35641 accagccatt gaatgacagc ccggcgtttt tctcctcatt aaccgccagc gcgccaaccc
    35701 gtcgcaacac gctttcagtc gtgcgaccac cacccgtggg gtgaacggct tgcagatgta
    35761 atcatccgcg cccatttcca gcccgaccac cacatcagac tcactcccca ttccggtcaa
    35821 catcaccaca ggcaatgccg gacgcagttt ctggatttgc tgcaataccc tcaggccgtt
    35881 ggtatcgggc agcatcatat ccagcaccac cagcgcaatg tcaggctcgc cctcaatacg
    35941 cgatatggcc tcctgtccgg tatggctgac tgaaaccgta aagacatgtt cacttaacac
    36001 gtcctgcaac agtgcgcaga tagccgtgtc atcatccacc accagcacga cgggtttcat
    36061 ggttacactc cgcatcattc atctaattaa tcattgtgcg tacttcttgt cgaagtgaaa
    36121 gcggaaagta atataaataa taaaaatata agtcaggtca cattattgat gatagtaata
    36181 aatatgccgt gcttttccct cttcagcaca aatgcctaat aaaatggcgc gttccgctca
    36241 cacttccact tattaaagtc gcaaattccc aattatgata accccagctt taaatgacgt
    36301 attttgcatg gattacatat ttacgacaca aatgataaat ggag
//
LOCUS       DAJTYG010000032        35891 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTYG010000032 DAJTYG010000000
VERSION     DAJTYG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.032"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(271..888)
                     /gene="tdk"
                     /locus_tag="OO568_001707"
     CDS             complement(271..888)
                     /gene="tdk"
                     /locus_tag="OO568_001707"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6641087.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQT
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1493..1906
                     /gene="hns"
                     /locus_tag="OO568_001708"
     CDS             1493..1906
                     /gene="hns"
                     /locus_tag="OO568_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6641088.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2050..2958)
                     /gene="galU"
                     /locus_tag="OO568_001709"
     CDS             complement(2050..2958)
                     /gene="galU"
                     /locus_tag="OO568_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6641089.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3160..4173)
                     /gene="rssB"
                     /locus_tag="OO568_001710"
     CDS             complement(3160..4173)
                     /gene="rssB"
                     /locus_tag="OO568_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6641090.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFHDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4265..5170)
                     /gene="rssA"
                     /locus_tag="OO568_001711"
     CDS             complement(4265..5170)
                     /gene="rssA"
                     /locus_tag="OO568_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6641091.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIIPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5283..5741
                     /locus_tag="OO568_001712"
     CDS             5283..5741
                     /locus_tag="OO568_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6641092.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLSVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5791..6633
                     /gene="purU"
                     /locus_tag="OO568_001713"
     CDS             5791..6633
                     /gene="purU"
                     /locus_tag="OO568_001713"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6641093.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6793..6877
                     /locus_tag="OO568_001714"
     tRNA            6793..6877
                     /locus_tag="OO568_001714"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6827..6829,aa:Tyr,seq:gta)
     gene            6924..7055
                     /locus_tag="OO568_001715"
     ncRNA           6924..7055
                     /ncRNA_class="other"
                     /locus_tag="OO568_001715"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7239..7916)
                     /gene="narI"
                     /locus_tag="OO568_001716"
     CDS             complement(7239..7916)
                     /gene="narI"
                     /locus_tag="OO568_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6641094.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7916..8626)
                     /gene="narJ"
                     /locus_tag="OO568_001717"
     CDS             complement(7916..8626)
                     /gene="narJ"
                     /locus_tag="OO568_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6641095.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8623..10161)
                     /gene="narH"
                     /locus_tag="OO568_001718"
     CDS             complement(8623..10161)
                     /gene="narH"
                     /locus_tag="OO568_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6641096.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10158..13901)
                     /locus_tag="OO568_001719"
     CDS             complement(10158..13901)
                     /locus_tag="OO568_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6641097.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQFLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14294..15685)
                     /gene="narK"
                     /locus_tag="OO568_001720"
     CDS             complement(14294..15685)
                     /gene="narK"
                     /locus_tag="OO568_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6641098.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16024..17820
                     /gene="narX"
                     /locus_tag="OO568_001721"
     CDS             16024..17820
                     /gene="narX"
                     /locus_tag="OO568_001721"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6641099.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17813..18463
                     /gene="narL"
                     /locus_tag="OO568_001722"
     CDS             17813..18463
                     /gene="narL"
                     /locus_tag="OO568_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6641100.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18464..19858)
                     /gene="ychO"
                     /locus_tag="OO568_001723"
     CDS             complement(18464..19858)
                     /gene="ychO"
                     /locus_tag="OO568_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6641101.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNFGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQMRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20043..20396
                     /gene="ychN"
                     /locus_tag="OO568_001724"
     CDS             20043..20396
                     /gene="ychN"
                     /locus_tag="OO568_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6641102.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20440..21135)
                     /gene="chaC"
                     /locus_tag="OO568_001725"
     CDS             complement(20440..21135)
                     /gene="chaC"
                     /locus_tag="OO568_001725"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6641103.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21293..21523)
                     /gene="chaB"
                     /locus_tag="OO568_001726"
     CDS             complement(21293..21523)
                     /gene="chaB"
                     /locus_tag="OO568_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6641104.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21793..22893
                     /gene="chaA"
                     /locus_tag="OO568_001727"
     CDS             21793..22893
                     /gene="chaA"
                     /locus_tag="OO568_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6641105.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGFLSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23224..23331
                     /locus_tag="OO568_001728"
     CDS             23224..23331
                     /locus_tag="OO568_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6641106.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(23480..24334)
                     /gene="kdsA"
                     /locus_tag="OO568_001729"
     CDS             complement(23480..24334)
                     /gene="kdsA"
                     /locus_tag="OO568_001729"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6641107.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24370..25179)
                     /locus_tag="OO568_001730"
     CDS             complement(24370..25179)
                     /locus_tag="OO568_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR6641108.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25183..25575)
                     /locus_tag="OO568_001731"
     CDS             complement(25183..25575)
                     /locus_tag="OO568_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6641109.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25572..26405)
                     /gene="prmC"
                     /locus_tag="OO568_001732"
     CDS             complement(25572..26405)
                     /gene="prmC"
                     /locus_tag="OO568_001732"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6641110.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26405..27487)
                     /gene="prfA"
                     /locus_tag="OO568_001733"
     CDS             complement(26405..27487)
                     /gene="prfA"
                     /locus_tag="OO568_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6641111.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27529..28785)
                     /gene="hemA"
                     /locus_tag="OO568_001734"
     CDS             complement(27529..28785)
                     /gene="hemA"
                     /locus_tag="OO568_001734"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6641112.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKNLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            28999..29622
                     /gene="lolB"
                     /locus_tag="OO568_001735"
     CDS             28999..29622
                     /gene="lolB"
                     /locus_tag="OO568_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6641113.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29622..30473
                     /gene="ispE"
                     /locus_tag="OO568_001736"
     CDS             29622..30473
                     /gene="ispE"
                     /locus_tag="OO568_001736"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6641114.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPSVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLKYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30624..31571
                     /gene="prs"
                     /locus_tag="OO568_001737"
     CDS             30624..31571
                     /gene="prs"
                     /locus_tag="OO568_001737"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6641115.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31696..33375
                     /gene="dauA"
                     /locus_tag="OO568_001738"
     CDS             31696..33375
                     /gene="dauA"
                     /locus_tag="OO568_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6641116.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33430..33708)
                     /gene="ychH"
                     /locus_tag="OO568_001739"
     CDS             complement(33430..33708)
                     /gene="ychH"
                     /locus_tag="OO568_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6641117.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            33986..34570
                     /gene="pth"
                     /locus_tag="OO568_001740"
     CDS             33986..34570
                     /gene="pth"
                     /locus_tag="OO568_001740"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6641118.1"
                     /translation="MTIKLIVGLENPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34687..35778
                     /gene="ychF"
                     /locus_tag="OO568_001741"
     CDS             34687..35778
                     /gene="ychF"
                     /locus_tag="OO568_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6641119.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         8824 a         9361 c         8807 g         8899 t
ORIGIN      
        1 ccttctgggg ccgttttttt gtttacccaa agcaactttt ccataaaccg acagcattag
       61 ccttcatcat atttgcgacg atgtataacg cctaaacaca gggatattgt actttacagg
      121 tcacaagtca acgtcggtgc ttaagagccc tgtgaggcgt atagcggcgt taaaaaactg
      181 ccgagaaggg tatatagccc ggaagaagtg cgtaaaacga actgacagga taaaagtgcc
      241 ctgctcaccc tgtcagtaaa gaaattctta ttaatcgtgg cgatgccttt cctgaatagc
      301 cgttaatgag ccgacttgta acgcctcttt atagtgttta cggcatacag aaacgtatcg
      361 ttcattacca ccaattacca cctgctcacc ttcgttataa ggtctgcctg tttgatcaag
      421 acgcagcacc atgcttgctt tacggccaca aaaacagatg gtttttaatt caaccagttt
      481 gtcggaccat gccagtaagt attggctgcc aataaataat tcacctcgaa aatcggtacg
      541 taaaccataa caaagtacgg gtatatcgag ttgatcgaca acctccgata attcatatac
      601 ttgttgtctg gttaaaaact ggcattcatc aaccagtacg caatgaattg cctgctgttc
      661 atgttccgca cgaatctcat caaataatga tgaattttgg ttaaataatt ttgcaggcga
      721 tgacaaacct atacgcgaac tgactttccc ggcaccaaag cgatcatcaa tttctgccgt
      781 atatacgaca gtgcgcatgc cgcgttcctg gtaattgtat gaagattgca acaatgctgt
      841 agacttaccc gcattcattg cggaatagta gaaatatagc tgtgccatca gccacaggcc
      901 ctcaatgata tgaaataaag ttggctggag tttatcataa ttcgtcagtt tttcagctca
      961 acagtatgca cagaagtggg tatatgcgtt ctcccttacg aagccttgca taatccttct
     1021 gagctatcat tacaactgcc tcgcttgtta taagcgggta aatgactgct ggtaactatt
     1081 cacaatcttt aacctgttgc gcaagtaata gccctctgtt gacctccagg agatagtgca
     1141 atactaagtc catgctctta ttgcgactgt tctacttttc atcattcgct taatagggaa
     1201 ttctcgtaaa cacaactaat acagaagact gaaaggtcgt cagcctacga taatctcccc
     1261 ataaaatgtg acatgaatca ggaagtttta acctcacgtg ctgcgaaatc atcggtgtaa
     1321 atagggctat atgccgcgtc ttttctggct aattttatga aaagatattt attggcggca
     1381 caaaataaag aacaattttg aattccttac attcctggct attgcacaac tgaatttaag
     1441 gctctattat tacctcaaca aaccacccca atataagttt gagattacta caatgagcga
     1501 agcacttaaa attctgaaca acatccgtac tcttcgtgcg caggcaagag aatgtacact
     1561 tgaaacgctg gaagaaatgc tggaaaaatt agaagttgtt gttaacgaac gtcgcgaaga
     1621 agaaagcgcg gctgctgctg aagttgaaga gcgcactcgt aagctgcagc aatatcgcga
     1681 aatgctgatc gctgacggta ttgacccgaa cgaactgctg aatagccttg ctgccgttaa
     1741 atctggcacc aaagctaaac gtgctcagcg tccggcaaaa tatagctacg ttgacgaaaa
     1801 cggcgaaact aaaacctgga ctggccaagg ccgtactcca gctgtaatca aaaaagcaat
     1861 ggatgagcaa ggtaaatccc tcgacgattt cctgatcaag caataatctt ttgtagattg
     1921 cacttgctta aaatcccgcc agcggcggga ttttttattg tccggtttaa gacaatttaa
     1981 taagaataag ctataaaaaa acggcgtcga ttgctcaacg ccgtttcgtg gataacaccg
     2041 atacggatgt tacttcttaa tgcccatctc ttcttcaagc caggctttaa attccgtgcc
     2101 aagggtgtta tgacgaatac cgtattcaac gaaggcctgc atgtaaccta atttattacc
     2161 gcagtcatgg ctcttccctt tcatatgata ggcttccacc gtttcttttt cgatcagcat
     2221 atcaattgcg tcggtgagct gaatttcatc accagctccc ggaggggttt ttgccagcaa
     2281 cggccaaata tccgcgctaa gtacgtaacg acccacaata gcgagattag acggcgcaac
     2341 atccgctttc ggtttttcta ccacaccaac catcggtacg ctttcacccg gcgctaattc
     2401 aacgcctttg caatccacaa cgccatatgc ggtcacatca gcaaccggtt caaccatgat
     2461 ctggctatga cccgtttcat caaagcggcg gatcatctct gccaggttat cctgtgacaa
     2521 atcggattca tattcatcca gaataacatc aggcaaaata acagctaccg gttcatcacc
     2581 cactaccggg tgagcacaca ataccgcgtg tcccaggcct ttcgccagac cctgacgaac
     2641 ttgcataata gtcacgtgcg gtggacaaat agactgcact tcatcaagca gttgacgttt
     2701 tacacgtttt tccagcattg cctccagttc aaaactggta tcaaagtggt tttcaataga
     2761 gtttttagat gagtgtgtaa ccagcacaat ttcagtaatg ccagccgcaa tacattcatt
     2821 cacgacgtat tgaattaatg gcttatcgac aagtggcagc atctctttcg ggatggcttt
     2881 cgtcgccggc aacatcctgg ttcctaatcc cgcaacgggg ataacggctt ttttgacttt
     2941 cgtattaatg gcagccattt aaattctcct ggactgttcg tgttttgaac gtgttcatat
     3001 ttctgtatcg catcccagta tatcagcacc ccgaaggggc taagttctga aaaggaggcg
     3061 agatagcata attaagacaa ttacttatac gaatggcgac gatagtacca ccagccgaca
     3121 ttagcaggta atgcaaattt agcccgcgtt atcgtttgct cattctgcag acaacatcaa
     3181 gcgcagtcga ccaccggttc cccatatttg gcattgccag gcatcgcatc gctggctcaa
     3241 ttgattcaaa taagcgttac ctaaagtgcc taacggaaca ccattactga tttgcacctg
     3301 gtgttcgccg gtatttaacg tcgcattcag acccgcagaa accagaatga gatttttcag
     3361 ttcgcgatga taatagccaa ctaatagcgg aaactgcccc ggcagattgg cctgacgaag
     3421 taaatggttt acctgcttca ataacgcgcc caactctggc aaccgttgat tttggtgtgc
     3481 aagctgttcc tgtaataatc cgttaaacaa tgcgcgtaat aacaaggcag caagtacgcc
     3541 attatgtcca gctcgggtga catcaaggca ataaaatgcc agatcgtttt ccgaaagtgc
     3601 ggcaatatca agcaccaggc cgggtttgtc cgcggcaacc aattgacggt aattaacccg
     3661 gcaatgggaa atcacctgct gaaccggcgg ttgtagttcc tgtaataatt tcgccgctgc
     3721 ggcagggtta tcaaccattg catcccagtc gtgaaaaagc ctttcctctt cctcaacgcg
     3781 cgaattaaac atgctgggat agagacaggc aaaaaccatc tcgcgcaagc gattcagatc
     3841 tttaactggt ttcagcaaaa catcttcaac gcccagacgt aacgctttgg caatatctgc
     3901 catattttca gtggcagata tcaccagaac tggggtctgg tcgcctctgt tacgtatatg
     3961 ctccagcagt ttaagcccgt tcattcgtgg catcgcgata tcacatatca tcaggtctgg
     4021 agtgaaacct cccagcaact caagggcatc caccccatca gccgccagta ccgttgtcgc
     4081 tcccaatgag gaaaaccatg aatccagaag cgagcgaaat acctgctcat cttcaacaat
     4141 gagaatctgt tttccgacca atggctgcgt catgttctct cccctgaccg gctttactca
     4201 atagtggcat gctattgcca cctgcgcctg tcagaattaa cttaagtgta gataaaaatt
     4261 ctggtcaaat gttggtgcgt accaacggca aaagttcgtc cattttcctt tccactgcca
     4321 gctgtccggc cgcaatggca gcgtgcgcgc gatggaaatc aagcgtagat atttgcgggc
     4381 aaacaggttg aatcagaata tcgggcggat cacctgccat gcggttcctt ttaaggcggt
     4441 tctccaacac ctggatagac gtggtcataa tctctgttgc cgttggcgct gtcacagccc
     4501 gacgtgtcgt tatgctgcct aacctttctt tcagacgcgc atgccacggt agagaatcac
     4561 cattctcgct attttcttca ctgacattaa aggagagcag atcttgttgc atcaaatgag
     4621 catcgtgctg caggtcgacc gctatcacaa tatcagcccc caatgcacgc gtaagagaaa
     4681 taggaattgg gttaactact gccccatcaa ccagccagta gccgttatgt gcaacgggtg
     4741 ccatgagtcc tggaatactg catgatgcgc gaatagcaag atggagatcg ccttcagtaa
     4801 accataattc acgccccgta cttaaattgg tggcaacagc cgcaaagcga cgggaacaat
     4861 tttcgatctc tgtttccggt attatttcgc gatattgatt gaagacacgc tcgccgcgca
     4921 gtaacccacc gcgctgccag gagaggtcca tcaggcgtaa aacgtcccaa taactgaaag
     4981 aggttaccca atcttccagc gcagataatc gatcgcatgc ataggccgcg cccaccagcg
     5041 aaccaattga acatcctgca acgatatcaa tttcaatacc cacttttttt agcgcattaa
     5101 taacgccaat atgcgaccaa cctctcgccg cgccagatcc cagcgccagc cctattttta
     5161 tctttctcat tattcccgca agattcccct ggaatgcaat cgtagccaca tcgctactgc
     5221 tcggttaaca tagtgctact caggcggtta atacgccgtt attgtttcca gggagattat
     5281 tggtgtctca gctttgtccc tgtggtagtg ctgtcgagta tagcctatgt tgccaccctt
     5341 atgtgtctgg tgaaaaggtt gcacctgatc cagaacatct catgcgttcg cgttactgcg
     5401 cttttgtgat gcaagacgct gattatttaa taaagacctg gcatccctct tgtggagcag
     5461 cggcgttacg tgccgaattg atgaccggat ttgcgcatac tgaatggttg ggattgagcg
     5521 tatttgaaca ttgttggcag gacgccgaca atatcggatt tgtcagtttt gttgctcgat
     5581 ttaccgaagg tggcaaaaca ggtgcaataa ttgaacgttc gcgatttttg aaagaaaacg
     5641 gtcaatggta ctatattgac ggtacacgtc cgcagtttgg tcgcaacgat ccctgccctt
     5701 gtggttcagg taaaaaattt aaaaagtgct gcggccaata atggttgacg gtacggttta
     5761 gcaaacactc tcaacaaggt ttttccagca atgcattcac tccaacgtaa agttctgcgt
     5821 actatttgtc cggaccaaaa aggtctgatc gcacgtatta ccaatatttg ctacaagcac
     5881 gagttaaata tcgtacagaa caatgaattt gttgatcacc gtaccgggcg cttttttatg
     5941 cgcacggaac tggaagggat ttttaatgat tccaccctgc tggcggatct cgatagcgca
     6001 ttgccagaag gctccgtgcg tgagctgaat cctgccggtc gtcgccggat agtgattctg
     6061 gtcactaaag aagcgcattg ccttggcgat ttgttgatga aagccaatta cggcggtctg
     6121 gatgtcgaaa tcgcggcagt tattggtaac cacgatactt tacgttctct ggttgagcgt
     6181 tttgatattc cgtttgagct ggtaagccat gaagggttaa gccgcaacga gcacgatcaa
     6241 aagatggcgg acgccattga tgcttatcaa cctgactatg tggtgctggc gaagtatatg
     6301 cgggtattaa cgccggaatt tgtggcacgc ttcccgaata aaatcatcaa tattcaccat
     6361 tcattcctgc ctgcgtttat cggcgcacgt ccttatcacc aggcctatga acgtggtgtg
     6421 aagattattg gcgcaaccgc tcactatgtg aatgacaatc tggacgaagg cccaatcatc
     6481 atgcaggacg ttattcatgt cgatcatacc tacacagctg aagatatgat gcgcgcaggt
     6541 cgtgacgtcg agaaaaacgt cttaagtcgt gcgctataca aagtactggc gcaacgcgtc
     6601 tttgtttacg gtaatcggac gattattctt taatcgccag caaaaataac tggttacctt
     6661 taatccgtta cggatgaaaa ttacgcaacc agttcatttt tctcaacgta acactttaca
     6721 gcggcgcgtc atttgatatg atgcgccccg cttcccgata agggagctgg ctagtaaaaa
     6781 gcattacccc gtggtggggt tcccgagcgg ccaaagggag cagactgtaa atctgccgtc
     6841 atcgacttcg aaggttcgaa tccttccccc accaccatca ctttcaaaag ccccggaatt
     6901 ctcaaacgaa tctgcaatca aatattctgc ccaagcgggg aaggatgaga agcttcgacc
     6961 aaggttcgac ttgagcgcca gcgagagagc gttgccgcag gcaacgaccc gaagggcgaa
     7021 gcgcgcagcg ctgagtaatc cttcccccac caccatcgct tccttgaata atcaaaacct
     7081 gttactccga gctaattttg cccgatggca ccacgtttac cgggcctaca atatcctccc
     7141 ctgtaggtcg gataagcgaa gcgcatccga caactcaccc cgcaccgtgc ccataaaaaa
     7201 accccgccga agcagggtct atgtgaacta aaattcgttt agtgacgagc gcgcaccagc
     7261 tggtacttac gtgtcagata ctccaccggt acgctccaga tgtgtaccag acgcgagaac
     7321 gggaacagca ggaataacgt catccccagc accaggtgca gacggaagat aaacgccaca
     7381 ccatcgaggt gttgagaagc gccaccgtgg aaggtcacca ccgactgcgc ccagccaacc
     7441 agtttcatca tctcgctacc gtccatatgc tgagcggaga acggaatggt cagcaggccc
     7501 agcgcgcact ggataacgag cagcgacagg atcaggatat ccgctccggt agtggttgca
     7561 cgcacgcgtg ggctgaacat acgacgtttc agcaaaagca cgccgccaat cagacacagc
     7621 acgccgctgg caccaccagc aaacatcgcc attttctgtt tcacttcaat cggcagccag
     7681 gcttcataca tccagtgcgg cgtcaacata ccgaagaagt gaccgacaaa aatccccaga
     7741 ataccgatat ggaacaggtt cgacgccagg ttcatccctt tgcgatccag catctggctg
     7801 gacgccgcgc gccaggtgta ctgcccgtag tcataacgca gccagctacc aatcaggaag
     7861 accgccccgg ctatgtacgg gtagatatca aagaagaaca tattcaggaa ttgcattagt
     7921 gctgtcctcc ggtggtgata ttcagatatt gcggcgcgac ggcaccggca aagcgacgct
     7981 gatgagcagt gattgctgaa tcaccgcagc ctttgtcagc aaagaattta acctgctctt
     8041 cttcccagac agcatccagc gcctgcggcg tatcatcgcg cgcttcgtcg gcaatttttt
     8101 ccgccacttt gtcgctgtcg atagcggtat tggccaattt cagcagcaga tcaaacatca
     8161 cggcataacg gctttcacgc tgttgcagac gcgcgctcag caatgccaga atcggcgcga
     8221 tatctttcaa accttccacg gcttcgcttt gcggcagctg cgacaggtac tccagataca
     8281 gcggcagatg gtccggtaat tcgcggctgt ttaactgcaa gccgtgctgc tcgtactgcg
     8341 ccagcaggtc caccatcgcc tggccgcggt cacgggattc accgtgaaca tgttcaaata
     8401 gcaacagtga cgtggcgcgg ccacggtcga acagttcgct gtactggacc tgggcatcga
     8461 gcggatccat cgccgttaaa tcgcgcagga aaatgcccag cgcatgggca tcttctttcg
     8521 acagattttt cgacgcagca atcgcctcaa acatctcttg ttgatgctgc cataaggcag
     8581 catccggata ttcaaggaga cgcgatacaa tcacgagttc gatcatggat gcggctccgt
     8641 tttgctggtc acatcgatgg catcgatacg acggctgttg aacagattga atttggtatc
     8701 tgagccgtgg cagccatcac caaaggtaaa gccgcagcca tttttctccg ggaaggcttc
     8761 ccgtgccagt tcacgatgac tactcggcac cacaaagcga tcttcgtagt tagcaatcgc
     8821 cagataacgg tacatctcct gtgcctgggc ttcggtcaga ccgacctctt ccagcgcacg
     8881 ggtatcaact ttaccgtcaa cagtttcagc acgtttgtaa tgacgcatcg ccagcatacg
     8941 tttcagcgca cgcagtaccg gtttggtatc accggcggtc agcagattcg ccagatactg
     9001 taccggaata cgcaagcttt cgacgtctgg cagaattccg ttgctgccca gctcgcccgc
     9061 gtctgctgca gactgaatcg gagacagagg cggcacgtac cagaccatcg gcagtgtgcg
     9121 atattctgga tgcagcggca gcgccagttt ccattccatc gccattttat aaaccggcga
     9181 ctgctgtgca gcttcaataa cgctcagcgg aataccgtct ttaatcgcct gctcgatgac
     9241 tttcggatcg ttcggatcga ggaacacgtc cagctgacgc tggtagagat ctttctcgtt
     9301 ctcggtgctg gctgcacgtt caatcgcgtc ggcatcgtac agcagcacgc caagataacg
     9361 gatacgaccg acacaggttt ctgagcacac ggtcggctga cccgcttcaa tacgcggata
     9421 gcagaagatg cacttctcag acttaccgct cttccagttg aagtagattt ttttgtacgg
     9481 acatccggtg atgcacatac gccagccacg gcatttatcc tggtcgatca ggacgatgcc
     9541 atcttcttca cgcttgtaaa tcgcaccgct cgggcaggtc gccacacatg ccgggttcag
     9601 gcagtgttcg cacaggcgcg gcaaatacat cataaaggtg ttttcgaact ggctatacat
     9661 cgccttctgg atgttgtcga agttcttgtc tttcgccagt ttatcaaact caccgcccag
     9721 gtcatcttcc cagttcggcc ctttttcgat tttcgccatc cgttccccgg taatcagcga
     9781 acgcggacgg gcaatcggct gcgatttgct gccttccggc gcggtatgca ggttctgata
     9841 atcaaaatcg aacggctcgt aataatcgtc gatccccggc agatgcgggt tagcaaagat
     9901 tttacccagc agcatggctc ggttacccat gcgcggttgc agtttgccat tgattttacg
     9961 gatccagccg cctttgtatt tttcctggtt ttcccagtca gtcgggaagc cctggcccgg
    10021 cttggtttcc acgttgttga accacgcgta ttccacgcct tcacggctgg tccagacgtt
    10081 tttacaggta actgaacagg tgtggcagcc gatgcactta tcgagattca gcaccatgcc
    10141 gacttgtgaa cgaattttca ttttacgctc tcctgtacct ggtcattgcc ttcgccatct
    10201 aaccagtcaa tgttcttcat cttacgcact acaacaaact catcgcggtt agaacctacg
    10261 gtgccgtagt agttaaagcc gtatgccaga tgggcatagc cgccgatcat atgcgtcggt
    10321 ttcggcgtga tacgggtgac cgagttatgg ataccaccac gctgttgggt aatttccgaa
    10381 ccaggcaggt taacgatacg ttcctgcgcg tggtacatca tggtcatccc tgccggaaca
    10441 cgctggctga caaccgcacg ggcagtcagt gcaccgttgc tgttgaagac ttcaatccag
    10501 tcgttatcgg cgatacccag atctttggca tcggcttcac tcaaccagac caccggacca
    10561 ccgcgaccta aagtcagcat cagcaggttg tcgctgtagg tggagtggat accccacttc
    10621 tggtgcggcg tcaggaagtt gagcgctttt tccgggttgc cgttggattt ctggcctatc
    10681 acttctttca ccgaacgggt gtcgatcggc ggacgataaa ccagcaggct ttcaccgaaa
    10741 tcacgcatcc actggtgatc ctgatacagt tgctgacgac cagagagcgt acgccatggg
    10801 atcagctcgt gaacgttggt gtaaccggcg ttgtaagaaa cgtgttcatc ttccagacca
    10861 gaccaggtcg ggctggagat aattttgcgc ggctgtgcct gaatatcgcg gaagcggatc
    10921 ttctcgtctt ctttattcag cgccagatgc gtatggtcac gaccggtaaa ttcgctcagg
    10981 gcagcccagg ctttcacggc tacctgaccg ttggtttccg gtgccagcgt caggatcatc
    11041 tctgccgcat caattgcggt gttcagcatc ggctggcctt ttgccggacc ttccgctttg
    11101 gtgtagttga gcttacgcag cagatccatc tcgctctggg tgttccaggc aatcccttta
    11161 ccgccgttac cgattttctc catcagcggg ccgatagagg taaagcgttc gtaagttgcc
    11221 ggataatcgc gctctacgac cataatgtgc ggtgcagttt tgcctgggat caggtcgcat
    11281 tcgccttttt tccagtcttt cacatccagc ggctgcgcca gttcagcggc agagtcgtgc
    11341 tggataggca gcgtgacgat gtcggtttct ttacccagat ggccgacgca cacttcggag
    11401 aatttcttcg cgatggcttt gtagatttcc cagtcgcttt tagcttccca ggccggatcg
    11461 accgccgcag acagcgggtg aataaacgga tgcatatccg aagtattcat gtcgtctttt
    11521 tcgtaccagg tcgccgtcgg caaaatgatg tcggaataga gacaggtgct cgacagacgg
    11581 aagtccagcg taaccaccag atccagcttg ccttccagac cattgtcctg ccagtccact
    11641 tcttccggct tcacgccgcc ctgttgcccc agatctttac cctggatacc gtgctccgtc
    11701 cccagcaggt acttgagcat aaactcatga cctttaccgg aagaaccgag caggttagaa
    11761 cgccagatga acaggttgcg cgggtggttt ttaccgtttt ctggttgttc tgccgcaaaa
    11821 cggatggaac cctctttcag ggatttcacc gtatagtcca ccggattcat cccggctttt
    11881 tccgcttcgc cagcgatagt cagcgggtta gtgcctaact gcggtgcaga cggcagccag
    11941 cccatgcgtt ccgcacggac gttaaagtcg atcaagtgtc cggtatagcg ggatttgtcc
    12001 gccatcggtg acagcaactc ttccgccgtg acggtttcat aacgccactg gctggagtgg
    12061 ttatagaaat aagaagtgct gttcatgtga cgcgccggac gctgccagtc aagggcaaac
    12121 gccagcggct gccagccggt ttgcggacgc agtttttcct gacctacata gtgcgcccag
    12181 ccgcccccgc tctgaccgac acagccgcag aaaatcagca tgttgatcag accacgatag
    12241 ttcatatcga ggtgatacca gtggttcagc cccgcaccga cgataatcat cgaacgaccg
    12301 tgcgttttat cagcgttatc ggcaaattca cgggcgatgc gaataatctg gctgcgagaa
    12361 acgccggtaa tctgctcggc ccaggccggg gtataagctt tcacatcgtc atagctggtt
    12421 gcacagttaa cgtcgttcag gccacgttcc agaccgtagt ttgccagcgt cagatcataa
    12481 acggtggtca ccagggcggt gctgccatca gccagttgca ggcgtttcac cggcagtttg
    12541 tgcagcagca cgttttccag ttccactttg ttgaagtgtt ccgtgccgtc gccaccaaag
    12601 tacgggaagc ctacctcagc gatctcatcc tggctaccca gcaggctcag ttgcagctcg
    12661 gtttcttcgc cagttttgcc gtcgcgctgc tcaagattcc atttgccctt ctcgccccag
    12721 cggaagccaa tagaaccgtt cggcgcaacc atttcgccat tggtattaaa ggcgacagtt
    12781 ttccattccg gattgttttc ctggcccagc gcatcaacca gatcagcagc gcgcagcata
    12841 cgacctgcag cgtagtaacc gtcgcgttct tccagcatca ccagcatcgg catgtcggtg
    12901 tagcgacgca catagtcggt gaaatactgg cttgggttgt cgaggtggaa ttcacgcagc
    12961 attacgtggc ccatcgccag cgccattgcc gcatcggtgc cctgtttcgg tgccagccac
    13021 agatcgcaca gtttggcgat ttcagcgtag tctggtgtga cggcaacagt tttggtgcct
    13081 ttgtaacgca cttcagtaaa gaagtgagca tccggggtac gcgtctgcgg cacgtttgaa
    13141 ccccaggcga tgatgtagct ggagttatac cagtcagcag attccggtac gtcagtttgc
    13201 tcgccccagg tttgcggaga cgcaggaggc aggtcgcagt accagtcgta gaagcttaag
    13261 caagtaccgc caatcagcga gagatagcgt gcacccgatg cgtaagaaac catcgacatt
    13321 gccggaattg gcgagaaacc agcaacacgg tccgggccgt agtttttgat ggtgtaaacg
    13381 ttagatgcgg cgatcagttc gttcacctcc tgccaagaag aacgaacaaa tccaccgcgt
    13441 ccacgcgcct gcttaaagct tttcgcttta tcggcgtctt caatgataga agcccatgcc
    13501 tcaaccggat cgctatgcag cgccttcgct tcacgccaca ttttcatcag gcgtttgcgc
    13561 atcatcgggt atttcaggcg gttggcactg taaagatacc aggagtagct ggcaccgcgc
    13621 gggcagccgc gaggttcatg gtttggcaga tccggacggg tacgcggata gtcagtctgc
    13681 tgggtttccc aggtgaccag accgtttttg acgtagattt tccagctgca ggagccggtg
    13741 cagtttaccc cgtgggtaga gcggacgatt ttgtcatgct gccaacgctg gcgatatcca
    13801 tcctcccagt cacggttggt attgagaaac tggccatgcc catcggcaaa ggtttcaccc
    13861 ttctgtttga agtagcgaaa ccggtccagg aatttactca tcggttttct cctgtgggag
    13921 cctgtcggcc ctctgataaa tcgacattgc taatgtttaa gggcaaggta acgctctgaa
    13981 acagcgtggg aattgataac gatcaagagt gatggggatg aaaaataaag taaattccta
    14041 agatacccct ttaggataat taacagttac agcataacat gctgtaaatt aagacgtttt
    14101 atacctgtag ttatttaaac tattaagatt tttatgcaaa tgggtattaa ggagtattcc
    14161 ccatgggtaa catattaatg gctcttaatg aaatccggcg aaataagcca tataacacta
    14221 atatgttgta actaaatgac gcataaaaaa agcgcggcat catgccgcgc aaaggataat
    14281 caaatattaa gatttatttt ttagaatgcc gaccatatac cgcccaagta atcactacac
    14341 aggcgatata gaaaatcaaa aatactttca ttgcacctac tggcgaaccc gttaatgcca
    14401 gcgagctacc aaacgctttc gggataaaga agccaccaat cgcgccaatc gcagagatga
    14461 aacccagcgc cgctgccgtg ccggttgccg cttcacgcat cgcacgttcg tcagaacccc
    14521 cttctgcttt cacgcgatcc attgtcagtt tacggaagat cactgaaatc atctggaaag
    14581 tggaaccact acccagccca gctgtcagga acagcgccag gaagaccgcg aagaacgcca
    14641 tgaagcttcc gccctgcccg tcagtcggta aggtcaggaa cagcaggccg ctgaaaatcg
    14701 ccatcagaat aaagttcacc agcgtgacac gagttccgcc cagacggtca gataatgcac
    14761 cacctgcaga acgcgccagc gcaccaataa acggcccgaa gaaagcgtat tgcagaatct
    14821 gaacatccgg gaactgcgtt tttgacagca tagcaaagcc cgcggagaag ccgatgaagg
    14881 agccgaaggt tgccagatac agcaggctca taatccacag atgaccccgt ttgagtaccg
    14941 gcaactgctc cttgatggag gctttcgagg tagcaagatc gttcatgcca aaccacgccg
    15001 caatggtgaa gatggcaagg aacggcaccc atatccagga cgcattcgcc agatacagct
    15061 cagtcccatc cggctgtttg acgccctggc taccaaatac tgcgaaaatc gacagtgata
    15121 ccaccagcgg agcaaccaac tgcatgacgc tgacgcccat gtttcccaga ccaccattca
    15181 gacccagcgc gccaccctgc ttctgtttcg gaaagaagaa gctgatgttt gccatactgg
    15241 atgcgaagtt cgcgccagca aaaccgcata gcagagagat gatgatgaag acgctataag
    15301 gcgtggaggt atcctgcacg gcaaaaccca gccagacgca aggaataatc agaataccgg
    15361 tgctgaacgc cgtccaacga cgaccaccga agataggaac cataaaggag tatggaacac
    15421 gtaataacgc gccagaaacc gaaggcagcg cagtcaacat aaatagctga tcggtcgtaa
    15481 aattaaaacc gactttcggt aagttcaccg caacagcgct gaacaacatc catacgcaaa
    15541 acgccagcag cagacaggga acggaaatcc acaggttgcg gctggcaata cgttgaccgc
    15601 gttgttgcca gaacgcagga tcttccggtc gccaatctgt tatgacagct ccagtagccc
    15661 tttcggaggc agatgagtga ctcatagaca cctctgatac tcgtttcgat gtctgccacc
    15721 ttagtgtctg gagctaaagg caatttgatg taaatcaaac gataagataa ctttatctat
    15781 cattgatatt tatcattacc catagtgagt acagtgattt cataaaaatt atgagatttt
    15841 tcacggtgct gtagaatccc tacccttacc gatgtaaagc gactaaccac acggcaaata
    15901 aggagtaact ctttccggct atgggtatac ttcagccaat agccgagaat actgccattc
    15961 cagaatatat cgtcacattc attaaggtta ttgctcattt aaagcctgaa ggaaggggtt
    16021 tacatgctta aacgttgtct ctctccgctc accctggtta atcaggttgc gcttattgtg
    16081 ttgctttcta ctgctattgg actggcaggg atggcggttt ctggctggct ggtgcaaggc
    16141 gttcagggca gcgcccatgc gatcaacaaa gcgggatcgc tgcgcatgca aagttaccgt
    16201 ctgttggcgg cagtgccatt aagcgagaaa gacaagccct taattaaaga gatggaacaa
    16261 acggcattta gcgccgagtt gactcgagca gcagaacgag acggacaact ggcgcaatta
    16321 cagggtttac aagattactg gcgtaatgaa ctgatccctg cgctgatgcg tgcacaaaac
    16381 cgagaaacgg tgtcagcgga tgtcagccag tttgttgccg ggcttgatca gctggtatct
    16441 ggttttgacc gcaccacgga aatgcgtatt gagacagtgg tactggtcca tcgggtaatg
    16501 gcggtattta tggcactttt actggtgttc actattatct ggttgcgggc gcgactgcta
    16561 caaccgtggc ggcaactgct ggcaatggcg agtgccgtca gccatcgcga ttttacccaa
    16621 cgcgcaaaca tcagcgggcg caacgaaatg gcgatgcttg gaactgcgtt gaacaatatg
    16681 tctgcagaac tggccgaaag ttatgccgta cttgagcagc gggttcagga gaaaaccgcc
    16741 gggctggagc ataaaaatca gatcctctct tttttatggc aggctaaccg ccgtttgcat
    16801 tcccgcgccc cgctgtgtga acgcctgtca ccggtactca acggcttaca gaatttaacc
    16861 ctgctacgtg atatcgaact gcgggtgtat gacactgatg atgaagagaa tcatcaggag
    16921 tttacctgcc agccagatat gacttgtgat gataaaggct gccagctctg cccgcgcggc
    16981 gtattacccg ttggtgatcg cggcacgacc ctgaagtggc ggctggctga ctctcatacg
    17041 cagtacggta ttttgctggc gaccctgccg caggggcgtc atcttagcca tgatcaacaa
    17101 caactggtgg ataccctggt tgaacaactt accgccacgc tggcgctgga tcgccatcag
    17161 gaacgtcagc aacagttgat cgtgatggaa gagcgtgcca ccattgcgcg cgaactgcat
    17221 gattctattg cccaatctct ctcttgcatg aagatgcagg tgagttgttt acagatgcag
    17281 ggcgatgcgc tgccagaaag cagccgcgaa ctgttaagtc agatccgtaa cgaactgaat
    17341 gcatcctggg cgcagttgcg tgaattgctc accacattcc gcttgcagct caccgagcct
    17401 ggattacgtc cggcgctgga agcgagttgc gaagagtaca gcgccaaatt tggcttcccg
    17461 gtgaagctgg attatcaatt gccgcctcgt ctggtgcctt cacatcaggc aatccacttg
    17521 ttgcaaattg cccgtgaggc attaagtaac gccctcaaac attcgcaagc gagtgaggtc
    17581 gtggtgacgg tggcgcaaaa cgataatcag gtcaaactga ccgtccagga taacggctgc
    17641 ggcgtgcctg aaaatgccat ccgcagcaat cactacggca tgataataat gcgcgaccgt
    17701 gcgcaaagtt tacgaggcga ttgccgcgtc cgccgtcgtg aatcaggtgg caccgaagtg
    17761 gtggtcacct ttattcccga aaaaactttc acagacgtcc aaggagatac ccatgagtaa
    17821 tcaggaaccg gctactatcc tgctgattga cgatcacccg atgctgcgaa ctggcgtaaa
    17881 acagcttatc agtatggcac cagatatcac cgtggttggc gaagcgagta atggcgaaca
    17941 gggtattgaa ctggcggaat ctcttgatcc cgatctgatc ctgttagatc tcaatatgcc
    18001 cggcatgaac ggtctggaaa cgctggataa actgcgcgaa aagtccctct cagggcgcat
    18061 tgtggtattc agcgtctcta accatgaaga agatgtggtc accgcactga aacgcggcgc
    18121 ggatggctat ctgttaaaag atatggaacc ggaagatctg ctgaaagcat tgcatcaggc
    18181 agctgctggc gaaatggtat taagcgaagc attaacgcct gttctggccg ccagcttgcg
    18241 cgctaaccgt gccactactg agcgcgatgt taaccagtta accccacgcg agcgcgatat
    18301 tctcaagctg attgcccagg gtttgccgaa caagatgatt gcccgccgcc tggatatcac
    18361 cgaaagcaca gtaaaagtgc acgtcaagca catgctgaag aaaatgaagc tcaagtctcg
    18421 cgtggaagca gcggtatggg tgcatcagga gcgcattttc tgattacggt tcccagcgca
    18481 gttcgtcgtt tgacaatgca tcgaacggtt cgacaagcgt tagcgtgatc tcattggagg
    18541 agacacgctg cccttggtta tcttccacca ccacagacaa tcgccagtga ttgcttgccc
    18601 cttccccgtt ctgccagtca ggcatgatca gcgtccagcc ctccgcgctg ttggcttgtg
    18661 cgcccggcgt caaacttaat atctgcgtat cgccctgcca aatcagttgc cgaataccgt
    18721 aacgactgcg catttgtaat ttcagcggca ctgtttcgcc aggttttaga tcccacggcg
    18781 gtgtcgccag aaacaccgtt aacgtttttc gctgtcggta ctcaagagtc ggtagattat
    18841 ttcgctgcgg attgtcatag cgactaccac gtaacgactg actttcggca acctcgcccg
    18901 cagaaagttg ttttttgagc ggtacaccaa agcggtaatt aagattcagc ccgaggttat
    18961 tttgattttc gccactttcc ccctgtttat gctgggccgt cacagtgact aatggcacag
    19021 gggtgtaatt taatcccaga ctcaacgcga cgggattgtg ataacccgta ccaaagttaa
    19081 acaaatcaac acgatcacca aaatactgtt ctaggctgac actggtattg aggtgttgat
    19141 agaacggcat gcgcatccga gctgtcaggt cgtacccgcg cgccatccgt tgttcctgcg
    19201 tggctgtctg ttcatgccat gcagcaaacg gctgataaaa gtttgccgat aatcgcaaat
    19261 attcgcccca cgcttcggca ccaaagcccg ctcgctgaag attttcgtcc tgcaagttgt
    19321 cataaaaagt gttataaccc accagccagt tgccgcgcgc ccagcgttgc ccaacgccca
    19381 cattgctcac caacccatta tcctgctgag taagaccaag ctggctccag gtgagataac
    19441 gatcattatc ttgtaacggc acaaaccagc ttccacgact gccggtgaaa tgtccttcgt
    19501 tatccacttt gacgtcaaca ctggcatttc cccacggtga tagccaggac tctacgtgct
    19561 gattaacctg ttgactaagc gcgtcgcgga cttttcccaa tgcgaaagct tttgcctgct
    19621 cgccagtatc cagcccgtta tcattcatac tggtttcgcc gaaatctttc acaatttcag
    19681 caaagtgttt ttccccatca tgattttcgg gagccatgcc taaatccggc agaccatcat
    19741 tgttattatc aaagggattt gccgcttttt gctcgaaggt agattgtgcg ttagctgtac
    19801 cgcctgctac aagcaagagt aaataaaacg ggataatgcg aggaacgaaa cggctcaaaa
    19861 cgtcggtaac ttccggtaat aatgaaaaac catagcctta aagaataacg attactggca
    19921 acttttgtcg tattttctcg cgctttcagg aaaatcctta aaaatgtgac cgattggtca
    19981 gacgctgcag aagcagtaag atagaagatg agtgtctaat tccctgaata caggagtaaa
    20041 taatgcaaaa aatcgtgatc gttgccaatg gcgcacctta cgggagcgaa tccttgttta
    20101 acagcttgcg gctggccatt gcgttacgag agcaggagag caatctggat ctgcgtctgt
    20161 tcctgatgtc tgatgcggtc acagccgggt tgcgcgggca aaaaccaggg gaaggctaca
    20221 acattcagca aatgctggag atccttaccg ctcagaatgt accggtgaaa ttgtgcaaaa
    20281 cctgtaccga cgggcgcggg attagcacac ttcctctgat tgatggggtg gaaatcggta
    20341 ctcttgtgga actggcgcaa tggacgctgt cagccgataa agtgctcaca ttttaatgtt
    20401 taagggggcg tctgacgccc cactatgagg gaagtttact caggcgaatc ccggccttaa
    20461 cacaccatcc ggataattct ccgccagtag tttttttacc gataccagca aatcattcag
    20521 cccatcgtcc tgcattccca gtttgataag ctcctgttcc agtgaaaaca ggtattgtgc
    20581 attggttccc agcggaccgc tcgccgccgc aatcaacggc gcgatgacct gagcgcgagt
    20641 gtcagattca tattctggat gtcgcgggtc cataataaac acaatggcgt ttactgtgcg
    20701 tccatcatca agatcaagct gacaccaggt tggcagataa cagccggtaa tcatctcgcg
    20761 cttccacaac agggttagtt cctgctccag cgtctcttct ggtagtcgat aggcgacgcc
    20821 tgtggtgcgt ccgccctctt tcagtgcaag catccgtccc ggctggtgcg cagtcccgcg
    20881 cccggcggtc aggcgcaggc aaaacgcgcg atgccatcca accagtgtac cggtgcatga
    20941 ttcggtaaac tccagtgccg gattccacat caacgatcca taaccgaaga tccacaccgg
    21001 tccctcatca ggtcgacaag ccagcgtcgc cgccagcgaa gccgcccgtt gttctgctga
    21061 ccataagagt gattcttcaa tggcaccaaa tgccgtttta caatcggcat tcatcaagaa
    21121 atcacgcgtt atcaccttac acctccgcca ctgcatactt ccctgcgaca actttttggt
    21181 gtcttccaga catttttacc gctcattttg aaaccactaa cgggacaata tgcaatccac
    21241 ggaccacttg caaggcaaca ccgcacgaat agctttaact aagctaccgg ttttacgatt
    21301 ttttatgcca tttatcatca tcccctttgg catattcatg cttcacagca gcccaggcca
    21361 ctttatgcgc ggtttcttcg cgactggcgt catcacgccg atcttcttta tctttatatt
    21421 gatcccatgc gctgttgaac gcttctttat agatatcctg ggcatgagac ggtagaacgt
    21481 gctttacgct ttccggcaga tcgcttttcg ttttatacgg catcgttgac tccttttaca
    21541 aagttgaaca ttaagtatgg tcaacaatat ctactcttgc caaaacataa gaattttcat
    21601 gtcagactgt tcttaagaaa cgctaaaaca aacattgtct aaacgtaata cttattttta
    21661 tgccccaatg agtaaaaaat cgacagcggg gtaaaaattc gtaaaatgtt accgatatcc
    21721 agacactttc tgttattttt cctacctatt ttttacattt ccgataaatt catctgcaaa
    21781 gggataacca taatgtcaaa tgctcaagag gcggtaaaaa cccgccacaa ggagacttcg
    21841 cttattttcc cggttctggc gctggtagtg ctgttcctgt ggggaagtag ccagacacta
    21901 ccagtggtca ttgccatcaa tcttcttgcg cttattggtt ttttaagtag cgcctttagt
    21961 gttgtccgtc atgcggacgt attagcccat cgcctgggag aaccttacgg ttcgcttatt
    22021 cttagccttt cagtggttat tcttgaagtc agtttgattt cagctttaat ggcaaccggc
    22081 gacgccgcgc caacgctaat gcgtgatacg ctctattcaa tcattatgat tgttaccggt
    22141 gggctggttg gcttttcatt attgttgggc ggtcgtaagt ttgccaccca atatatgaat
    22201 ctgtttggta tcaagcagta tttaattgcg ctgttccccc tggcgataat cgtactggta
    22261 tttccaatgg ctctgcctgc ggcgaatttt tcaaccggtc aggcgttact ggtagcatta
    22321 atttctgcgg caatgtatgg cgtatttttg ctgatccagg ccaaaacgca tcaaagtttg
    22381 tttgtctacg agcacgaaga tgacagtgat gacgacgacc cgcaccacgg taaaccgtct
    22441 gcccatagca gcctgtggca tgctatctgg ttgattatcc atctgattgc cgttattgca
    22501 gtcaccaaaa tgaacgccag ctcgctggag acattgctcg acagcatgaa tgcccctgtt
    22561 gcctttactg gcttcctggt ggcactgttg attctgtcgc cggaaggttt aggtgcgtta
    22621 aaagcagtgt tgaacaacca ggttcagcgc gcgatgaatc tgttctttgg ttcagtgtta
    22681 gcaacaattt cgcttaccgt acctgtcgtc acgctaattg cctttatgac gggtaacgaa
    22741 ttgcagtttg cacttggtgc gccagaaatg gtggtgatgg tggcctcttt agtgctgtgc
    22801 catatctcct tctccaccgg acgtactaac gtactcaatg gcgcagcgca tctggcactg
    22861 tttgccgcct atttgatgac gatatttgcc tgacagttat atctcctccg atgacggaaa
    22921 tcgggagaga taagataaat tcctgcttat tgtatttctt catactacct ggacagctcg
    22981 ttaatcagaa ttgcgagcct gtaccacaac ctgcacgaaa ccactgcaac agctgttgaa
    23041 aagccggaat tatccggtga ggcgcaatgt tgcgggggct ttatccctgg tggcattggt
    23101 tgctggaaag agaaaacccc cgcacgttga gaggtaaaac ctgacaacaa aacgggggct
    23161 aatcttgaaa ccagacactt caagaatagc cgcaaaatgt tgtcattaca acaaggcggc
    23221 tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc accgatcctg
    23281 gcggggatta ttaccgcagc gattgtcagc tggtggcgta accggaagta aggtgaaaac
    23341 gggcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg gtaacagcct
    23401 gcggcctttt ttgtatctgg cacccatgaa aaaagtctta acgcagaacg ctaagacttt
    23461 atttttcaag caaaaaagat tacttgctgg tatccagttc ttcgaaacct ttcaccagat
    23521 catcaatcgc tttcatctgc ttgaggaacg gttccagttt agccagcggc agcgcggatg
    23581 gaccatcaca tttcgcatgt tccggatccg gatgcgcttc aataaacagc cccgccagac
    23641 ctaccgccat accggctcgt gccagctcag ccacctgagc acgacgacca ccggaagctg
    23701 cgccaaacgg atcgcggcat tgcagtgcgt gggtcacgtc gaaaatcacc ggcgagttac
    23761 cagacacttt cttcataatg ctgaagccca gcatatcgac aaccaggttg tcatagccga
    23821 agttagcacc acgatcgcaa agaatcactt tttcgttgcc gccttctttg aatttatcaa
    23881 cgatattacc catctgtccc gggctgacaa actgtggttt cttgacgtta attaccgcac
    23941 cggttttcgc catggcttca accaggtcag tctggcgagc aagaaacgcc ggcaactgaa
    24001 tcacatccac gacatcagca acgggctgtg cctgacttgg ttcgtgaacg tcggtgataa
    24061 ttttcacgcc aaaagtctgc ttcaactcct ggaagatttt catcccttct tccaggcccg
    24121 gtccacgata agagtggatg gaggagcggt tggctttgtc aaaagaggct ttgaacacgt
    24181 aagggatacc cagtttctgg gtcacagtta cgtagtgctc gcaaatgcgc atcgccagat
    24241 cgcgagattc cagcacgttc ataccgccaa acagtacgaa cggcaggtca tttgctacgt
    24301 tgatgtcgcc aatgctaacc actttttgtt tcataggatc gccttaatct tgagtaaaat
    24361 gtcgattaat taatgcagca caatatgttt atgcgcgatg ttatttattt gcgcacggat
    24421 catttcgctg atcgggtctt ccggacactg ttcaacgaaa taacttaaat cgttcaacgc
    24481 aacgtgttcg caatccagtt gcgcataaat caacccgcga tcgcgaattt catagggatc
    24541 ttcagggttg aattgtaata aagcttcgct ggtgcgtaac gccagctcca tctgattttc
    24601 ttccatcaac gaggctttga gtgtatccag caatttgcgg attacctcaa tgttatcagc
    24661 ttcatcaagg tcttcataaa acagttccgc cgacgggctg atatttccct ttaaccatac
    24721 gtccagcata tgttcgctta acgattcacc gttaaaagga ttaatcagcc aaatttcgcc
    24781 atccggacat tcaatgcgca atatcagctg cgtagggaaa atcaccggca gcagcggcaa
    24841 atcgagacga ttcgcgaccc ataataaaac cgcacctaat gataccgcac tgccctgtcg
    24901 attctttaac acctggtcca gccataatgc atcggaaaga cgataaacgc cgcgtgaggc
    24961 tttaaatccc cagtcgccgt aaaacagcgc gatcagtttt tccagctgct cttctaaagg
    25021 cagaagctgg ctgatttctt ccttcgccag actaacgaga cgctccagtt cgtcgtaaac
    25081 atcttgcgag ggaaaatcgc ggcggattgc ttcgcaagcc aggatcatgc cttcgcacaa
    25141 tggcgcttta ttaaattcga aatcagctaa cgatctcatg acttacccca gtaacggtac
    25201 ttttgtggtg gcgagtttaa tgatgatgta cagcaccacc aacgccagcg ggaaggcaat
    25261 aatgcgcgcc tgctgactgt gcatacgacg ataatcgagt gcaataaaac ccaaaacgat
    25321 ataaatgata actccaaaca gcttttcagt cagccactgt gcctgcccgg aaaatggcag
    25381 gatgtgcgct ttagccatca acgcaatgcc gcttaacaat aacaccgtat cgacaaccgg
    25441 cggcacaatg cgcgtccagc gagcaaatgc ctgtggatgc ttctgataac gtagccagaa
    25501 acgtaaggtt aatagcccaa cagaaagcgc gatactaata agatgaacac taagcagtgt
    25561 agaaaaactt gtcattgata atagcggccg agcgttacgc gctcgttatc accatagtca
    25621 cggcaggttt cgacgtcatg ataccccgcg aggataaatg cttgtcgcac cgcttcgccc
    25681 tgctgccagc catgttccag aagcagaaag ccgccggata ccagcgcgtt acgcgactgt
    25741 tcgatgatat gcacgatgtc tgccattcca ctgtctgccg caaccagcgc agtgagcggc
    25801 tcaaagcgga catcgccttg ttgaagatgt gggtcctgct cgtcaatata cggcggattg
    25861 ctgacaatca tcgcaaactg ctgcccggct agcgcgctaa accagtcgct ttgcagaatg
    25921 tggatatttt tgatcgccag atgctgggca ttacgttgtg ccagggagac agcatcaggc
    25981 atacgatcta cagcgataat ttcgcagtcc gggcgctcgc tagccagcgc aagcgcaatc
    26041 gccccggtac ccgtcccgag atcgagaata cggcaaggtt gttcaggcaa ccgcgccagt
    26101 gcctgctcca ccagacactc cgtatccggg cgcggaatta aggtcgctgg agaaacaaat
    26161 aacggcaacg accagaattc tcgcaccccg gttaaatgag caatgggttc accatcgcga
    26221 cgacgtgtca gtagcgcatc aagttgctga cattgttcgt cagtcagctg cgtttcacca
    26281 aaggcgagaa taaaagtacg ccctctgccg gtaacatgct ccagcaggat ttcagcatca
    26341 cgccgcgggc tttcgctcgc ctgaagttgg cttattgctt cacgtaacca gtgttgatat
    26401 tccattattc ctgctcggac aacgccgcca gttggtcggc ctgatgttcc tggataatcg
    26461 gttcaatcag catatccagc ttaccttcca tcacttcatc caggcggtag agcgtcaggt
    26521 tgatgcggtg atcggtaacg cgcccctgcg ggaaattgta agtacggtta cggtcgctgc
    26581 gatcgccact ccccagcagg ttacgacggg tagacgcttc ggcctgttgg cgttttgcca
    26641 tttcagcagc gtggatgcga gcaccgagca ctgaaagcgc cttcgctttg tttttatgtt
    26701 gtgaacgttc gtcctgacat tcaacaacaa tcccggtcgg caagtgagta atacgaattg
    26761 ccgaatcggt ggtgttaacg tgctgaccac ccgcccctga cgagcggaaa gtatcaatgc
    26821 gtaaatctgc tgggttgatg tccggcagtt ctgcgtcagg cagttctggc attaccgcaa
    26881 cggtacaagc agaagtatga atacgaccct gcgattccgt agcaggaaca cgttgcacgc
    26941 gatgaccgcc ggattcaaat ttcagacgac catacacacc atcaccgcta attttggcga
    27001 tgatctcttt ataaccacca tgttcaccct cgctggcgct catgatttct acccgccagc
    27061 ggcgggcttc ggcgtaacgg ctgtacatac ggaacagatc gcctgcaaac agcgctgctt
    27121 cgtcgccgcc ggttccggca cggacttcga ggaaggcgtt acgttcgtca tcaggatctt
    27181 ttggtagtaa tagaacctgt aattgctgtt ccagttgctc gcttttttct ttagcttcgc
    27241 gcagttcatc ctgcgccatc tcacgcattt caggatcgtc gagcatcatc tgtgcggttt
    27301 cgatatcttc ctgaacctgt tgccagtcgg taaaacagcg cgaaacatca cttaactgcg
    27361 catattcgcg tgataatgcg cgaaaacgtt cctggtcggc gatagtttgc gcgtcaccca
    27421 gcaacgcctg aacttcttca tggcgttcat gcagggcttc cagtttggca acgatagaag
    27481 gcttcatagg cgtaaatgca ccctgtaaaa aaagaaaatg atgtactgct actccagccc
    27541 gaggctgtcg cgcagaatat tcaggcgttc gttatccccg tcacgggcgg cctgttgaag
    27601 tgatttcgtt ggcgcatgga tcaagcggtt agtcagtttc catgccagat cctgcataat
    27661 ggcttgcgcg tcgccgccct gctcaagggc cgctaacgct ttggcggtta actcatcgcg
    27721 aacgtgctct gcctggctgc gatactcgcg aatggtttcg ctggcgcttt gtgctcgcag
    27781 ccacgccata aattcgctgg tttcctgagc gacaatagtt tccgcctcaa ccgctgcggc
    27841 tttacgctgc gccaggttgt gcgaaatgat gctttgcaga tcgtccacac tataaagata
    27901 agcattcgcc agtttgccaa cttccggctc aacatcgcgc ggaacggcaa tatccaccaa
    27961 cagcattggt tggttacgac gactttttaa tgcgcgctcc accatgcctt tcccgataat
    28021 cggtaacggg ctggcggtgg aactgatgat gatatcggct tcgcgcagac gttcgtcgat
    28081 ctcactcagg gcaatcactt ccgcgccaac ttcatctgcc agaatttggg cacgttcgcg
    28141 agtgcggttg gcgataatca tcttctgtac tttatgttcg cgcagatgac gcgctaccag
    28201 ctcgatggtt tcgcccgcgc ctaccagcaa cactgtgacc gtagagagcg attcaaagat
    28261 ctgccgcgcc agcgtacaag ccgcaaaagc gacagacaca gcactggcac cgatatctgt
    28321 ttcagtgcga acgcgtttcg ctacagagaa agatttctgg aacatgcgtt ccagttcgct
    28381 ggccttcata tgaccttttt gcgaatcggc aaacgctttt ttaacctgac cgaggatctg
    28441 cggctcccca agaaccagtg aatccaggcc gctggcaaca cgcattaaat ggctaaccgc
    28501 gtcgttatcc tgatgccagt agaggttttt acgcagatct tcttcattaa gattgtgata
    28561 atcgcaaagc cagcggatta acgcctcttg caggttatcc tgctcttcaa cgctaagata
    28621 aagttccgtg cggttgcacg tcgacagcac cacgccgccc tgcaccatcg gctgcgcaag
    28681 caggctgtca agcgcctgat cgagcttatc cggcgaaaac gatacacgtt ctcgcagcga
    28741 tacaggtgcc gttttatggt tgataccgag tgctaaaagg gtcatgtctg cgggaaataa
    28801 taccaacgtt gatagggtta gtctgcttgc atcatacagg atgcgtaaga tcaataaaag
    28861 agagcgcccc cttttggagt aattgccgaa agccgttaga ttctggcaat taagacaact
    28921 tgaacataga cgatagcgga cggtaacgct agcattaagg gttataactg caacgtatct
    28981 caaggacttg tcatcactat gcccctgccc gattttcgtc ttatccgcct gctaccgctg
    29041 gctgctcttg tgctcactgc ctgttccgtt accacgccca aaggtcctgg caaaagcccg
    29101 gattcgccac aatggcgtca gcatcagcaa gacgtgcgca atcttaatca gtatcagact
    29161 cgcggcgcgt tcgcttatat ttctgaccaa caaaaagtgt acgcccgctt tttctggcag
    29221 caaaccggcc aggatcgcta ccgtctgctg ctcactaacc cattgggcag cacggaactg
    29281 gagctgaatg ctcaaccggg taacgtgcag ttagtcgaca ataaaggtca gcgttatacc
    29341 gccgatgacg ccgaagagat gattggcaaa ttgaccggaa tgccaattcc gctcaacagc
    29401 ttgcgccagt ggattttagg tttaccgggt gatgcaaccg actacaaact ggacgaccag
    29461 taccgcctga gcgaaattac ctacagccag aatggcaaaa actggaaggt tgtttatggt
    29521 ggttatgaca ccaaaacgca acctgcgatg ccagccaata tggaactcac cgacggtggt
    29581 caacgcatca agttaaaaat ggataactgg atagtgaaat aatgcggaca cagtggccct
    29641 ctccggcaaa acttaatctg tttttataca ttaccggtca gcgtgcggat ggttaccaca
    29701 cgctgcaaac gctgtttcag tttcttgatt acggcgacac catcagcatt gagcttcgtg
    29761 acgatgggga tattcgtctg ttaacgcccg ttgaaggcgt ggaacatgaa gataacctga
    29821 tcgttcgcgc agcgcggttg ttgatgaaaa ctgcggcaga cagcgggcgt cttccgacgg
    29881 gaagcggtgc gaatatcagc attgacaagc gtttgccgat gggcggcggt ctcggcggtg
    29941 gttcatccaa tgccgcgacg gtcctggtgg cattaaatca tctctggcaa tgcgggctaa
    30001 gcatggatga gctggcggaa atggggctga cgctgggcgc agatgttcct gtctttgttc
    30061 gggggcatgc cgcgtttgcc gaaggcgttg gtgaaatact aacgccggtg gatccgccag
    30121 agaagtggta tctggtggcg caccctagtg taagtattcc gactccggtg atttttaaag
    30181 atcctgaact cccgcgcaat acgccaaaaa ggtcaataga aacgttgcta aaatgtgaat
    30241 tcagcaatga ttgcgaggtt atcgcaagaa aacgttttcg cgaggttgat gcggtgcttt
    30301 cctggctgtt aaaatacgcc ccgtcgcgcc tgactgggac aggggcctgt gtctttgctg
    30361 aatttgatac agagtctgaa gcccgccagg tgctagagca agccccggaa tggctcaatg
    30421 gctttgtggc gaaaggcgtt aatctttccc cattgcacag agccatgctt taagccgggc
    30481 aagctgagtt tcggtgacaa cgtcaccttg ttccagacgt tgcatcgcgc tctttaatac
    30541 accgcctgga aaggatcatg cctggcccgc acagttttcg gcagattctt tccaccaatg
    30601 gacgcatgcc tgaggttctt ctcgtgcctg atatgaagct ttttgctggt aacgccaccc
    30661 cggtactagc acaacgtatt gccaaccgcc tgtacacttc actcggcgac gccgctgtag
    30721 gtcgctttag cgacggcgaa gtcagcgtac aaattaacga aaatgtacgc ggtggtgata
    30781 ttttcatcat ccagtccact tgtgccccta ctaacgacaa cctgatggaa ttagtcgtta
    30841 tggttgatgc cctgcgtcgt gcttccgcag gtcgtatcac cgctgttatc ccctactttg
    30901 gctatgcgcg ccaggaccgt cgcgtccgtt ccgctcgtgt accaatcact gcgaaagtgg
    30961 ttgcagactt cctctccagc gtcggtgttg accgtgtgct gacagtggat ctgcacgctg
    31021 aacagattca gggtttcttc gacgttccgg ttgataacgt atttggtagc ccgatcctgc
    31081 tggaagacat gctgcagctg aatctggata acccaattgt ggtttctccg gacatcggcg
    31141 gcgttgtgcg tgcccgcgct atcgctaagc tgctgaacga taccgatatg gcaatcatcg
    31201 acaaacgtcg tccgcgtgcg aacgtttccc aggtgatgca tatcatcggt gacgttgcag
    31261 gtcgtgactg cgtactggtc gatgatatga tcgacactgg cggtacgctg tgtaaagctg
    31321 ctgaagcgct gaaagaacgt ggtgctaaac gtgtatttgc gtacgcgact cacccgatct
    31381 tctctggcaa cgcggcgaac aacctgcgta actctgtaat tgatgaagtc gttgtctgcg
    31441 ataccattcc gctgagcgat gaaatcaaat cactgccgaa cgtgcgtact ctgaccctgt
    31501 caggtatgct ggccgaagcg attcgtcgta tcagcaacga agaatcgatc tctgccatgt
    31561 tcgaacacta atcgaacccg gctcaaagac ccgctgcggc gggttttttt gtctgtaata
    31621 tccatttgta tgacctatgc ctccttcacc tgccatttag ttgacagatg atgcgctcac
    31681 ggatgaaaca ttattgtgaa caaaatattt tcctcacatg tgatgccttt ccgcgctctg
    31741 atcgacgctt gctggaaaga aaaatatact gccgcacggt ttacccgtga cctgattgcc
    31801 gggataaccg tcgggattat tgctatcccg ctggcgatgg cgttggctat tggtagtggt
    31861 gtggcacccc agtacggttt atataccgca gctgttgcgg ggattgtcat tgctctaacg
    31921 ggtgggtcac gctttagcgt ttccggtccg actgcggcat ttgtggtaat tctctatccc
    31981 gtttcgcaac agtttggact ggcaggactg ctggttgcga ccttgttgtc gggaatcttt
    32041 ttgattctga tggggctggc acgctttggt cgtctgattg agtatattcc ggtttccgtc
    32101 accttaggtt tcacctcggg tatcgggatc accatcggta ccatgcagat taaagatttt
    32161 ctcggtctgc aaatggccca tgtcccggaa cattatctac aaaaagtcgg cgcattattt
    32221 atggcgctgc cgaccattaa tgtgggtgat gctgccattg gcattgtgac gcttggcatt
    32281 ctggttttct ggccgcgtct gggcattcgt ttacccggtc accttccggc cttgctggct
    32341 ggttgcgcgg tgatggggat tgttaacctg ctcggcggac atgttgctac catcggttcg
    32401 caattccact acgtcctggc cgatggttct cagggtaacg gtattccgca actgctgccg
    32461 caactggtgc tgccgtggga tctgcctaat tcagaattca cgctaacctg ggattctatt
    32521 cgcaccctgc tgcctgcggc attctcaatg gcaatgctcg gggcgatcga atctctgctc
    32581 tgcgctgtgg tactggatgg tatgaccggg actaagcaca aggcgaatag tgaactggtc
    32641 ggtcaggggc tggggaatat catcgctcca ttctttggtg gtattaccgc tactgctgcc
    32701 atcgcgcgtt ctgccgctaa cgtccgtgcc ggggcaactt cccctatctc ggcggtgatc
    32761 cactctattc tggttattct cgccctgctg gtactggcac cactgctctc ctggctgccg
    32821 ctttccgcca tggcagccct gctgttgatg gtggcgtgga acatgagtga agcgcacaaa
    32881 gtggtcgact tgctgcgtca tgcgccgaaa gatgacatca tcgtcatgct gctgtgtatg
    32941 tcactgaccg tgctgtttga tatggttatt gccatcagcg tggggatcgt gctggcatcg
    33001 ctgctgttta tgcgtcgtat cgcacgtatg actcgcctgg caccggtagt cgtagatgtt
    33061 ccagacgatg tcctggttct gcgcgttatt ggcccgctgt tttttgctgc tgctgaaggc
    33121 ttattcacgg acctggagtc acgtcttgaa ggcaaacgga ttgtgattct gaagtgggat
    33181 gccgttccgg tacttgatgc tggtggtctt gatgcgttcc agcgttttgt gaagcgtctg
    33241 cccgaaggat gtgaactgcg cgtgtgcaac gtggaattcc agccactgcg aactatggct
    33301 cgcgcaggca ttcaaccgat cccgggacgc ctcgcgttct tcccgaatcg tcgcgcggcg
    33361 atggcggatt tataagtacg agattgacca gtcagcgcaa actgactggt cagcaaactg
    33421 cattatttgt tagctatgac ggcgattatc gctacggcgg caacgtttgt catagtgacg
    33481 aacccaccag taacggtcgc acacctgttc atgcccgcca acacgggcac ccgccagcca
    33541 gagaatggca ccgacgaaaa tacttagcac tgcaccatgt gcgaaatact ggggcatatt
    33601 aaactgtggt aactggttga ggattgaata ccccacgccg accaccatta ccaccagacc
    33661 caaccccatg agcacgttac cgagtatcga agcgtttttg cgtttcatat gtcacctccg
    33721 gaactttctg ggttgtaaca gggaataccc ctcttcctta tgtgtaaagt atagacaaca
    33781 ccctgtgggt tatgtgcggg cgtaatcaca attacaaccc ttatttcaac aaaactttac
    33841 aaataaacgc ctgaacacct tgactttcta atagtccact caggcaatta ttacgttatc
    33901 tgcatttgat acgcagtttt ttgtcaagcg gggccgcaac cagtaaacta cgcgccagtt
    33961 atgtacacac tcaggacaaa aaaacgtgac gattaaattg attgtcggcc tggagaaccc
    34021 cggtgctgaa tacgccgcaa cgcgacataa tgctggtgcc tggttcgttg acttactggc
    34081 agagcgtttg cgcgctccgc tgcgcgaaga ggctaaattc tttggttata cttcgcgagt
    34141 cacccttgga ggcgaagatg tccgcctgtt agtcccgact acatttatga atctcagcgg
    34201 caaagccgtt gcggcgatgg ccagtttttt ccgcattaat ccggacgaaa ttctggtggc
    34261 ccacgacgaa ctggatctgc ctcctggcgt cgccaaattt aagttgggcg gtggccatgg
    34321 tggtcacaat ggactgaaag acatcatcag taaattgggt aataacccta actttcaccg
    34381 tttacgcatc ggaatcggtc atccgggcga taaaaataaa gttgtcggtt ttgtgttagg
    34441 caaaccgcct gttagtgaac agaagttaat tgatgaagcc atcgacgaag cggcgcgttg
    34501 tactgaaatg tggtttacag atggcttgac caaagcaacg aaccgattgc acgcctttaa
    34561 agcgcaataa gtcgttgtct gcggcatttt tgccgagtgc cgtgtataat aggcaaagtt
    34621 atttccattt ctgcaatctg ttagcaataa caggttgatt attaagatat taaggtgatt
    34681 taaatcatgg gattcaaatg cggtatcgtc ggtttgccca acgtcgggaa atctaccctg
    34741 ttcaacgcgc tgaccaaagc cggtattgaa gcggccaact ttccattctg caccattgag
    34801 ccgaacacag gcgtcgtacc aatgcctgac cctcgcctgg atcaactggc tgaaatcgta
    34861 aaaccgcaac gtacgcttcc cacgaccatg gaatttgtcg atatcgccgg tctggtaaaa
    34921 ggcgcatcga aaggcgaagg tctgggtaac cagttcctga ccaacatccg tgaaaccgaa
    34981 gcgatcggtc acgttgttcg ctgctttgaa aatgacaaca tcattcacgt ttccggcaaa
    35041 gttaacccgg ctgacgatat tgaagttatc aacaccgaac tggcgctggc agacctcgac
    35101 acctgcgaac gtgcgattca tcgcgtacag aagaaagcca aaggtggcga taaagacgcg
    35161 aaagctgagc tggcggtcct ggaaaaatgc ctgccccagc tggaaaacgc aggtatgctg
    35221 cgcgcgctgg atttaagcgc tgaagagaaa gcggctattc gttacctgag cttcctgacg
    35281 ctgaaaccaa caatgtacat agccaacgtc aacgaagacg gttttgaaaa caacccatat
    35341 cttgaccagg tgcgtgaaat cgcggcgaaa gaaggttctg ttgtggttcc ggtttgtgct
    35401 gctgttgaag cagacattgc cgaactggac gacgaagaac gtgacgagtt tatgcaggag
    35461 cttgggctgg aagaaccggg cctgaaccgt gtgatccgtg ccggttataa gctgctgaac
    35521 ctgcaaactt acttcaccgc tggggtgaaa gaagtgcgtg catggaccat tccggttgga
    35581 gcaaccgcgc cgcaggcagc gggcaaaatc catactgatt ttgaaaaagg ctttatccgt
    35641 gcacaaacca tctcgtttga agatttcatc acttacaaag gtgaacaagg cgcgaaagaa
    35701 gcaggcaaaa tgcgtgcaga aggtaaagat tacatcgtga aagatggcga tgtgatgaac
    35761 ttccttttca acgtctaatt aatttattgt ctcatgaggt ttcgctaaat ctcatgaaga
    35821 gcgcaaaagt atataaaatc cacgcaattg cgtggatttt tcatttcacc gagtcctaat
    35881 cattcttgta a
//
LOCUS       DAJTYG010000033        35255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTYG010000033 DAJTYG010000000
VERSION     DAJTYG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.033"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..1151)
                     /gene="setB"
                     /locus_tag="OO568_001742"
     CDS             complement(<1..1151)
                     /gene="setB"
                     /locus_tag="OO568_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6641120.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATL"
     gene            1519..2649
                     /gene="fruB"
                     /locus_tag="OO568_001743"
     CDS             1519..2649
                     /gene="fruB"
                     /locus_tag="OO568_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6641121.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2649..3587
                     /gene="fruK"
                     /locus_tag="OO568_001744"
     CDS             2649..3587
                     /gene="fruK"
                     /locus_tag="OO568_001744"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6641122.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3604..5295
                     /gene="fruA"
                     /locus_tag="OO568_001745"
     CDS             3604..5295
                     /gene="fruA"
                     /locus_tag="OO568_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6641123.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5719..6660
                     /locus_tag="OO568_001746"
     CDS             5719..6660
                     /locus_tag="OO568_001746"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6641124.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6648..7586
                     /gene="psuG"
                     /locus_tag="OO568_001747"
     CDS             6648..7586
                     /gene="psuG"
                     /locus_tag="OO568_001747"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6641125.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7680..8930
                     /locus_tag="OO568_001748"
     CDS             7680..8930
                     /locus_tag="OO568_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6641126.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFIA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9001..9699)
                     /gene="yeiL"
                     /locus_tag="OO568_001749"
     CDS             complement(9001..9699)
                     /gene="yeiL"
                     /locus_tag="OO568_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCR6641127.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLMNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLT
                     LEMDPENKFSGMMQ"
     gene            9830..10771
                     /gene="rihB"
                     /locus_tag="OO568_001750"
     CDS             9830..10771
                     /gene="rihB"
                     /locus_tag="OO568_001750"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6641128.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10871..12121
                     /locus_tag="OO568_001751"
     CDS             10871..12121
                     /locus_tag="OO568_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6641129.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLVQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12177..13265)
                     /locus_tag="OO568_001752"
     CDS             complement(12177..13265)
                     /locus_tag="OO568_001752"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6641130.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLVDCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13268..14125)
                     /gene="nfo"
                     /locus_tag="OO568_001753"
     CDS             complement(13268..14125)
                     /gene="nfo"
                     /locus_tag="OO568_001753"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6641131.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14199..15248)
                     /locus_tag="OO568_001754"
     CDS             complement(14199..15248)
                     /locus_tag="OO568_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6641132.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15347..16228
                     /locus_tag="OO568_001755"
     CDS             15347..16228
                     /locus_tag="OO568_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6641133.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16434..17903
                     /gene="lysP"
                     /locus_tag="OO568_001756"
     CDS             16434..17903
                     /gene="lysP"
                     /locus_tag="OO568_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6641134.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVLAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18195..20186
                     /gene="cirA"
                     /locus_tag="OO568_001757"
     CDS             18195..20186
                     /gene="cirA"
                     /locus_tag="OO568_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCR6641135.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20218..21054)
                     /gene="yeiG"
                     /locus_tag="OO568_001758"
     CDS             complement(20218..21054)
                     /gene="yeiG"
                     /locus_tag="OO568_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6641136.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATYYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSAPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            21312..21980
                     /gene="folE"
                     /locus_tag="OO568_001759"
     CDS             21312..21980
                     /gene="folE"
                     /locus_tag="OO568_001759"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6641137.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21997..23154
                     /locus_tag="OO568_001760"
     CDS             21997..23154
                     /locus_tag="OO568_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6641138.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGLAISKTSR"
     gene            23296..24335
                     /gene="galS"
                     /locus_tag="OO568_001761"
                     /pseudo
     CDS             23296..24335
                     /gene="galS"
                     /locus_tag="OO568_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            24615..25613
                     /gene="mglB"
                     /locus_tag="OO568_001762"
     CDS             24615..25613
                     /gene="mglB"
                     /locus_tag="OO568_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6641139.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25674..27194
                     /gene="mglA"
                     /locus_tag="OO568_001763"
     CDS             25674..27194
                     /gene="mglA"
                     /locus_tag="OO568_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6641140.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIGMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27210..28220
                     /gene="mglC"
                     /locus_tag="OO568_001764"
     CDS             27210..28220
                     /gene="mglC"
                     /locus_tag="OO568_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6641141.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28256..29491)
                     /gene="preA"
                     /locus_tag="OO568_001765"
     CDS             complement(28256..29491)
                     /gene="preA"
                     /locus_tag="OO568_001765"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR6641142.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVKKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(29485..30724)
                     /gene="preT"
                     /locus_tag="OO568_001766"
                     /pseudo
     CDS             complement(29485..30724)
                     /gene="preT"
                     /locus_tag="OO568_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            complement(30918..31157)
                     /locus_tag="OO568_001767"
     CDS             complement(30918..31157)
                     /locus_tag="OO568_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6641143.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31160..31879)
                     /gene="sanA"
                     /locus_tag="OO568_001768"
     CDS             complement(31160..31879)
                     /gene="sanA"
                     /locus_tag="OO568_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6641144.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32029..32913)
                     /gene="cdd"
                     /locus_tag="OO568_001769"
     CDS             complement(32029..32913)
                     /gene="cdd"
                     /locus_tag="OO568_001769"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6641145.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33043..33738)
                     /locus_tag="OO568_001770"
     CDS             complement(33043..33738)
                     /locus_tag="OO568_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6641146.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMSVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKVARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33735..34133)
                     /locus_tag="OO568_001771"
     CDS             complement(33735..34133)
                     /locus_tag="OO568_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6641147.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34372..>35255
                     /gene="dusC"
                     /locus_tag="OO568_001772"
     CDS             34372..>35255
                     /gene="dusC"
                     /locus_tag="OO568_001772"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6641148.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVR"
BASE COUNT         8662 a         8616 c         9307 g         8670 t
ORIGIN      
        1 agagtggcga taatcatcac catcgcaaac cagaacacag cgtgataatt ccagatctcg
       61 gcgacgatgc ccgccactga tcctgcgatg atccagccca cgcgcgacgt gttggtatag
      121 agcgtggtgg ctgaacctgc ctgaccgggc atcaaatcct gaaaatagag catcccgatg
      181 ccgcccagaa tgccaataaa aatagcattt agcagctgca agcccaacag aatggcaggt
      241 gaatgcgcca tcagcattcc tgcgtaaaaa cagacgccac ccacggcagc aacgcgcatt
      301 aagaaacgct tacccagacg tttggcgaaa tatccggcaa tcaacatcgt cgggatttcc
      361 agcccggcgg cggtccccat catcacaccg gccagtttct cgggaagatg cagttcgttg
      421 ataataaata gcggcatgtt gatgatgtac aggctgttcg agccccacat caatgtacaa
      481 atgacaaaca gcagtagcgt atcgcgacgg ttacggcgcg gcgcttcaac cgtgccggtc
      541 gccagcggaa gctctttttg catcgacggt aaaaacagcc acaccatcac gccgcaaaca
      601 ataaacgcta ccgctgcgct cagatacatt accgtaaagc tgaaacccat cgctaacgca
      661 taagccagcg gtgggccaat gacccatgcc agtgaaacct gagcgcgtaa aaaagagctg
      721 aacatcaccg cctcacgtcc ggttttgtcg gcatgttcac gggcaagggc aaacatttgc
      781 gggttagcgg tcgagccaaa gctgctaaga aagacgccaa cgaatagcaa aacaaagtag
      841 ttgcgattcc aggcaaaaag ggtgcaggcc agcacgccta acaggcagca aaagacaatc
      901 agcgatttgc gatcgccgcg cttatcagag cgcccagcga gaaactgact taccagaatc
      961 ccaatgacag cgctgccggt aaagaagaat cccaccatcg ccggacgggc atgtacttca
     1021 tcggtaagga aaatactgag tgtcggggtt tgcagagcac ccgcaatacc ggtgagaaag
     1081 gcaacgatta aaaacgccgt cgaggtcagg tcaaacgatt tcgcgctgga gactgcgggg
     1141 gagttatgca tgctggtcga tcactggaag aggaagacgc ggagtttacg ctgcttgtca
     1201 gaaaataaac agacagcgaa atcaaaagcg ccacgaataa tcaaaatgcc atttcactgt
     1261 tgctgattgg ctgctgacgc tgctgaaatg aaattgctga taacggattt tcccatcagc
     1321 aattaggaaa aatgacaaaa aattgtgcag cacatcaaac ttttgctcat aactttacgg
     1381 ctttccttgc gtgctgaaac agaagtatta tgctttcttg aaacgtttca gcgcgatctt
     1441 gtctttaacc ctaagccagg ttggcgcttt ttttctcata gaggctgaat cgtttcaatt
     1501 cagcaagaga ggagaactat gttccagtta tccgtacagg acatccatcc gggcgaaaag
     1561 gccggagaca aagaagaggc gattcgccag gtcgctgcgg cgctggtgca ggccggtaat
     1621 gtagcagaag gctacgtcaa tggcatgctg gcgcgcgaac agcaaacctc aacgttcctc
     1681 ggcaatggta ttgctattcc acacggcact accgacaccc gcgatcaggt gctgaaaacc
     1741 ggcgttcagg tatttcagtt cccggaaggc gtcacctggg gtgacggtca ggtagcgtac
     1801 gtggcaatcg gtattgctgc cagctcggat aaacatctgg gcctgctacg ccagctgacc
     1861 cacgtactga gcgatgattc cgttgctgaa caactgaagt cagcaacaac agcagaagaa
     1921 cttcgcgcat tactgatggg cgaaaagcag agtgagcagc tgaagctcga caacgaaatg
     1981 ctgacgctgg atatcgtcgc cagcgatctg ctgactcttc aggcgctgaa cgctgcgcgt
     2041 ctgaaagagg cgagggcagt tgacgccact ttcgtcacca aagccatcaa tgaacaaccg
     2101 ctgaatctcg gacagggtat ctggctgagc gatagcgccg aaggcaatct gcgtagtgcg
     2161 attgcggtaa gccgtgcggc aaatgctttt gatgtggacg gcgaaacggc agccatgctg
     2221 gtgagtgtgg cgatgaatga cgatcagccc atcgcggttc ttaagcgtct cgctgatttg
     2281 ttgctcgaca ataaagctga ccgcttgctg aaagcggatg cggcaacgtt gctggcgctg
     2341 ctgaccagcg atgatgcgcc gaccgacgac gtgttaagcg cggagtttgt ggtgcgcaat
     2401 gaacacggcc tgcatgctcg tccaggtacc atgctggtca ataccattaa acaatttaac
     2461 agtgatatta ccgtgacaaa ccttgatggc accggcaaac cggcaaacgg acgtagtctg
     2521 atgaaagttg tggcacttgg cgttaagaaa ggtcatcgcc tacgctttac cgcccagggt
     2581 gcagatgctg aacaggcgct gaaagccatt ggcgacgcta tcgctgctgg tcttggggag
     2641 ggcgcataat gagcagacgt gttgctacga tcacccttaa tccggcttat gaccttgttg
     2701 gtttctgccc ggaaattgaa cgcggcgaag tgaacctggt gaaaaccacc ggtctgcatg
     2761 cggcgggtaa aggcatcaac gtggccaaag tattaaaaga cctgggaatt gatgtcaccg
     2821 ttggcggctt cctgggtaaa gacaatcagg atggttttca gcaactgttc agcgagctgg
     2881 gcattgccaa ccgtttccag gttgtacagg ggcgcacccg aattaacgtt aagctgacgg
     2941 aaaaagacgg cgaagtgacc gacttcaact tctcgggttt tgaagtcacc cccgccgact
     3001 gggaacgctt tgtgactgat tctctgagct ggctcggtca gttcgatatg gtctgtgtca
     3061 gcggaagctt accgtcaggc gtcagcccgg aagcgttcac cgactggatg actcgcctgc
     3121 gtagtcagtg tccttgcatt atctttgata gtagccgtga agcgttagta gcaggtttga
     3181 aagcggcacc gtggctggtg aaacctaacc gccgcgagtt ggaaatctgg gcaggccgta
     3241 aactgcctga aatgaaagat gtgattgaag ctgcacatgc gctacgtgaa caaggtattg
     3301 cgcatgttgt tatttcactg ggtgccgaag gcgcgctttg ggttaatgcc tccggcgaat
     3361 ggatcgctaa accaccgtca gtcgatgtcg taagcaccgt tggcgcaggg gattctatgg
     3421 ttggtggcct gatttatggc ttgctgatgc gtgaatccag tgaacacaca ctgcgtctgg
     3481 cgacagctgt tgcagccctg gcggtaagtc aaagcaatgt gggtattacc gatcgtccgc
     3541 agttggccgc aatgatggcg cgcgtcgact tacaaccttt taactgacag caggagaggc
     3601 ataatgaaaa cgctgctgat tattgacgct aatctcggtc aggcacgcgc ctatatggcg
     3661 aagaccctgc tgggcgcggc ggcgcgaaaa gcaaaactgg aaatcatcga caatccgaac
     3721 gacgcagaaa tggcgattgt tctcggtgat tccatcccga atgacagcgc gctgaacggt
     3781 aaaaatgtct ggctgggtga tatttcccgg gcagttgcgc accctgagct gttcctgagt
     3841 gaagccaaag gccatgcgaa accttacact gcgccggtca ctgcgacagc accggttgcc
     3901 gccagcggtc cgaaacgcgt agttgcggtg actgcttgcc cgactggcgt agcacacacc
     3961 tttatggcgg ctgaagccat tgaaaccgaa gcgaaaaaac gtggctggtg ggtgaaagtt
     4021 gaaacccgtg gttctgttgg cgcgggtaat gcaatcactc ccgaggaagt agccgcagcg
     4081 gatctggtga ttgtggcggc agatatcgaa gtggatctgg cgaaatttgc tggtaaaccg
     4141 atgtatcgta cctctaccgg tctggcgctg aagaaaacct cgcaggaact ggataaagcg
     4201 gttgctgaag caacgccgta tgaaccggcg ggcaaagctc aaacggcgac cactgaaggt
     4261 aagaaagaga gtgcaggcgc ttatcgtcac ttgctaacgg gcgtctctta catgctgccg
     4321 atggtcgttg ctggtggtct gtgtatcgcg ctttcttttg cttttggtat cgaagcgttt
     4381 aaagagccgg gtacgttggc agcggcgctg atgcagattg gtggtggttc agcctttgcg
     4441 ctgatggtgc cggtactggc aggttatatt gccttctcca ttgccgatcg tccgggcctc
     4501 acgccgggtc tgattggcgg tatgctggcg gtcagcaccg gttctggctt cattggcggt
     4561 attattgcgg gcttcctggc tggttacatt gcgaagttaa tcagtacgca actgaaactg
     4621 ccgcagagta tggaggcgct gaaaccgatc ctgatcattc cgctaatttc cagtctggtg
     4681 gtcggtctgg cgatgatcta cctgatcggt aaaccagttg ctggcattct cgaagggctg
     4741 actcactggc tgcagaccat ggggactgcg aatgcggttc tgctgggggc gatcctcggt
     4801 ggcatgatgt gtactgacat gggcggtccg gtaaacaaag cagcgtacgc attcggtgtg
     4861 ggtctgctga gtactcaaac ctatggcccg atggcggcga ttatggcggc aggtatggtg
     4921 ccaccgctgg caatgggcct ggcaacaatg gtggcgcgtc gcaaattcga caaagcgcag
     4981 caggaaggtg gcaaagccgc tctggtattg gggctgtgct tcatttcgga aggtgcaatt
     5041 ccgtttgctg ctcgtgatcc gatgcgtgtg ctgccgtgct gtatcgtggg tggggcgctg
     5101 actggcgcaa tctcaatggc gattggtgcg aaactgatgg caccgcacgg tggtctgttt
     5161 gttctgctga tccctggcgc tattacgccg gtattgggtt acctggtagc aattattgcc
     5221 ggtacgctgg tggcgggttt ggcctatgcc ttcctgaaac gtccggaagt ggacgcagta
     5281 gcgaaagcag cgtaataaaa ggtgtgttac agggcagaaa tttcctgccc tgtaacattt
     5341 gatgagcgca aagagaatga ccagcttgtg aatgtaattt tatgaattta aatggtttta
     5401 tctttcagta gaaacttcaa acgttcatca aacactaagg ttatttcctc aagggcagat
     5461 tattttctgc ccttttttag tgttcctgtt ctgtgtatcc cgtcacaaat tcctcgcttt
     5521 tgtaattcag gcatttaatc gcattttgtg agcctgctca aatctgccca ttttgcctct
     5581 ctggtggtaa tttactttac gtaaaacatg catactatga gcacatgttt aaatatgaac
     5641 acatgtttaa accgtctttg cagtatctat attcttgtgg attgacaggg ttggtaattt
     5701 tttaccagag gacgtgctat gcgcgaaaag gattatgtcg taattatagg ttcggcgaat
     5761 attgatgtcg ccggatattc acatgaatca ttaaattatg cggattcaaa tccaggtaaa
     5821 ataaaattta cgcctggtgg agtagggcgc aatattgcac aaaacctggc gttgctgggt
     5881 aacaaagcct ggctactgag cgccgtaggc agtgattttt atggtcaatc gctgctaacg
     5941 caaaccaatc aatctggtgt ttatgtcgat aaatgcctga ttgtgccggg agaaaatacg
     6001 tcgagttatt tatcattact cgataatacc ggtgaaatgc tggttgctat aaatgacatg
     6061 aatattagca acgctattac agctgaatat ctcgcacagc accgtgaatt tattcagagg
     6121 gcaaaggtca ttgtcgcgga ctgtaatatc agtgaagagg cactggcatg gattctggat
     6181 aatgccgcca acgtacccgt atttgtcgat ccggtttcag cgtggaaatg tgtcaaagtg
     6241 cgcgaccgtc taaatcagat ccacactctg aagccaaacc gccttgaagc ggaaaccctg
     6301 agtgggattg cgctgtcagg gcgtgacgat gtggcaaaag ttgctgcctg gttccatcaa
     6361 catggcctga accgactggt attgagcatg ggcggcgacg gcgtttatta cagcgatatc
     6421 agcggtgaaa atggctggtc tgcgccgatc aaaaccaatg ttattaatgt taccggagcg
     6481 ggcgatgcca tgatggcggg acttgcttcg tgttgggtag acggaatgcc gtttgccgaa
     6541 tctgttcgtt tcgcacaggg atgttcgtca atggcgctct cctgtgaata caccaataac
     6601 cccgatttat cgattgccaa cgttatatcg ttagtggaga acgcagaatg tctgaattaa
     6661 aaatttcccc tgaattatta caaatttctc cggaagtgca ggacgcttta aaaaacaaaa
     6721 aaccggttgt ggcgctggaa tcaaccatta tttctcacgg gatgccgttc ccacaaaatg
     6781 cccagaccgc aattgaagta gaagaaacta ttcgtaaaca gggcgcagta cctgccacta
     6841 tcgccattat tggcggcgtg atgaaagtgg gtttaagcaa agaaaaaatt gaattactgg
     6901 gtcgtgaagg gcataacgtg accaaagtta gtcgtcgcga tttacctttt gttgttgccg
     6961 ccggaaaaaa tggcgcaacc actgtggctt caacgatgat tattgcggcg cttgccggaa
     7021 ttaaagtatt tgccaccggg ggaattggtg gtgtgcatcg cggggcggaa cataccttcg
     7081 atatttctgc cgatttgcaa gaactggcaa atactaatgt caccgttgtt tgtgccgggg
     7141 cgaaatctat tctcgattta ggattaacca ctgagtattt agaaaccttc ggtgtgccgt
     7201 taattggcta tcagactaaa gcgctgcctg cgtttttctg ccgtaccagc tcgtttgacg
     7261 tcagcattcg tctcgacagc gccagtgaaa ttgcccgtgc aatggcggtg aaatggcaaa
     7321 gcgggctgaa cggtggcctc gtggtagcga acccgatccc ggaacagttt gcgatgccgg
     7381 aggaatctat caatgcagcc atagatcaag ccgtcgccga agccgaagag cagggcgtta
     7441 ttggtaaaga aagtacaccg ttcctgctgg cgcgcgttgc tgagctgacc ggcggtgaca
     7501 gcctgaaatc caatatccag ctggtgttca acaacgccat tctggcgagt gaaattgcca
     7561 aagaatacca gcgtctcgcg ggttaatttc tgacgggcag aaagccgtcg cccgtactca
     7621 tcaagcatac caaaacctgg cgctgacgcc gggtttactg gcatgaaggg aaaatcatta
     7681 tggatataat gagaagtgtt gtggggatgg tggtgttact ggcaatagca tttctgttgt
     7741 cagtgaataa aaagagcatc agtttgcgca cggttggagc cgcactgctg ctgcaaatcg
     7801 ctattggtgg catcatgctc tacttcccac cgggaaaatg ggcagtagaa caggcggcat
     7861 taggcgttca taaagtgatg tcttacagtg atgccggtag cgccttcatt tttggttcgc
     7921 tggttgggcc gaaaatggat gtcctgtttg acggtgcggg ttttatcttc gcctttcgcg
     7981 tacttccggc gattattttc gttactgcgc tcatcagtct gctgtactac attggcgtga
     8041 tggggctgct gattcgcatc cttggcagca ttttccagaa agccctcaac atcagcaaaa
     8101 tcgaatcttt tgttgcggtt accactattt tcctcgggca aaatgagatc ccggcgatcg
     8161 ttaaaccgtt tatcgatcgc gtgaatcgca acgagttgtt taccgcaatt tgtagcggga
     8221 tggcgtccat tgctggttcg atgatgattg gttatgccgg aatgggcgta ccaattgact
     8281 acctgttagc ggcatcgctg atggcgatcc ccggcggtat tttgtttgca cgtattctta
     8341 gcccggcaac cgagccttcg caggtcacat ttgaaaatct gtcgttcagc gaaacgccgc
     8401 caaaaagctt tatcgaagcg gcggcgagcg gtgcgatgac cgggctgaaa atcgccgctg
     8461 gtgtagcgac ggtggtaatg gcgtttgtcg caattattgc gctgatcaac ggcattatcg
     8521 gcggaattgg cggctggttt ggtttcgcca atgcctctct ggaaagtatt tttggctatg
     8581 tgctggcacc gctggcgtgg atcatgggtg tggactggag tgatgccaac cttgctggta
     8641 gcctgattgg gcagaaactg gcgattaacg aattcatcgc ttacctgagt ttctccccat
     8701 acctgcaaac gggcggcacg ctggaagtga aaaccgtggc gattatatcc tttgcgcttt
     8761 gtggttttgc taactttggt tctatcggtg ttgtcgttgg cgcattttcg gctatttcgc
     8821 caaaacgtgc tccagaaatc gcccagcttg gtttacgggc gctggcagca gcaacgcttt
     8881 ccaacctgat gagtgcgact attgccgggt tctttattgg tctggcgtaa tgctcgattt
     8941 gtagaccgga taaaacgtat taagcgttgc atccggcaat cgcgctattg caaataattt
     9001 ttactgcatc atcccggaga atttattctc cgggtccatc tccagcgtca gtcctgacaa
     9061 ctgctttctg tttttaatta gatatccttt cttgctttta attaataaac cgtcgtgaat
     9121 gaattgcgcg agaacatata aaagatgtcg ataagaaacg cctaaatact ctgccgcttg
     9181 cgtatgcttt tcgtgataaa gatcaccttc ttgtgagagt aatataaatg ctgccaggcg
     9241 attcattaac ggaaatgatt gattctgagt taaagaaaca atattacgat aatttttatg
     9301 acttaaggtg acgcaaagtt ttcgtaaaaa tagggtgtcg tttaataaca gcggacggta
     9361 atgtttcata gggagcgcaa ggcaccaaca ctcttcaata gcctgcaccg cacgcggttc
     9421 atggtctttg tcgattaact caatctcgcc aataaaacag ggagcagcaa agaaatcgat
     9481 cagcgacacg cgaccattag caagcgtggc gtaaagcctg gcgcggcctc gcgtcagata
     9541 aaacagccat gacggctgtt gcccttcctg cacaatgtag tcacgcgcta aaaaatgaaa
     9601 cagccgcgta tcggctgaaa catccgttga aaagcaatcc ttaaacgcag attcactcat
     9661 caattgctgc ttaaggtcat tattgtggat ttctttcacg atgtttatcc gctatgagat
     9721 ttctcatatt tagcatacgt acgctgttgt taagattcaa tcatcagccc ggtcgaattt
     9781 tctttgctgt agaaaaaaat aacgcaactg gaaacagagg aaataaacaa tggaaaagag
     9841 aaaaattatt ctggattgtg agccgggtca tgatgatgct attgcaatga tgatggcagc
     9901 gaaacatccg gcaatagatt tattaggcat cactattgta gcgggtaatc agacgcttga
     9961 taaaacatta attaatggcc tgaatgtttg ccagaaactg gagattaatg ttccggttta
    10021 tgcggggatg ccgcaaccca ttatgcgtaa acaaatcgtt gccgataata ttcacggtga
    10081 aaccggactg gatggcccgg tattcgagcc gctgacccgc caggcagaaa gtacccatgc
    10141 ggtgaaatat atcatcgata ccctgatggc aagcgatggc gatatcactc tggtgccggt
    10201 tggtccgctt tcaaatatcg cggtggcaat gcgtatgcaa cccgcaatcc tgcccaaaat
    10261 ccgtgaaatt gtgctaatgg gcggcgctta cggtacaggc aacttcaccc catctgccga
    10321 gttcaatatc tttgccgacc cggaagccgc ccgcgtggtg ttcacctccg gcgttccatt
    10381 agtgatgatg ggcctcgatc ttaccaacca gaccgtttgc accccggatg taattgctcg
    10441 gatggaaagg gtaggcggcc ctgccggaga gctgttcagc gacatcatga acttcactct
    10501 caaaacgcag ttcgaatact acggcctggc tggcggcccg gtgcatgacg ccacctgcat
    10561 tggttatctg atcaaccctg atggcattaa aacccaggat atgtacgtcg aagtggacgt
    10621 caacagcggc ccttgctacg ggcgtacggt ctgcgacgaa ctgggcgttc ttggcaagcc
    10681 cgctaatacc aaagtcggca tcactattga taccgactgg ttctggggct tagtcgaaga
    10741 gtgcgtgcgc ggctacatca aaacccatta agtgttgata acaagccggg acagtatcgc
    10801 aagatgcagg cccggctatt tctgatgggc agaaagccgt cgcccaaaat gacatgaaga
    10861 gataatcact atggatgtca tgagaagtgt tctgggaatg gtggtactgc tggcaattgc
    10921 gtttttactg tcagtaaaca agaagaagat cagcctgcgt accgttggcg cggcgttagt
    10981 gttacaggtc gtgattggcg ggattatgct ttggttaccg ccagggcgtt gggtcgcaga
    11041 aaaagtcgct tttggcgtcc acaaagtgat ggcgtacagc gacgcaggta gcgcatttat
    11101 cttcggttct ctggtcggac cgaaaatgga taccttattt gatggcgcag gatttatctt
    11161 tggtttcagg gtattaccgg caattatctt cgtcactgca ctggtgagta ttctctacta
    11221 catcggtgtg atggggatct taattcgcat tctcggcggt atcttccaga aagctttaaa
    11281 tatcagcaag atcgagtcat tcgtcgcggt caccaccatt ttcctcgtgc aaaacgaaat
    11341 tccggcgatc gtcaaaccct ttatcgatcg tctgaatcgc aatgaattat ttacagcgat
    11401 ttgtagtggc atggcctcga ttgctggttc gacaatgatt ggttacgccg cactgggcgt
    11461 acctgtggaa tatttgctgg cggcatcgtt aatggcgatc cctggcggga tcttgtttgc
    11521 ccgcctgtta agcccggcta cggaatcttc gcaggtttct tttaataacc tctctttcac
    11581 cgaaacaccg ccaaaaagca ttattgaagc cgatgcgaca ggggcaatga ccgggctgaa
    11641 aatcgccgcc ggtgtagcga cagttgttat ggcatttgtc gccatcattg cgttaattaa
    11701 cggtattatc ggcggcgttg gcggctggtt tggttttgaa catgcctcgc tggagtccat
    11761 tttaggttac ctgctggccc cacttgcgtg ggtaatgggt gttgactgga gtgatgcaaa
    11821 tcttgccggg agtttgattg gacagaaact ggcaataaat gaatttgtcg cttatctcaa
    11881 tttctcaccc tatctgcaaa cggctggcac tctggatgct aaaaccgtgg cgattatttc
    11941 cttcgcgttg tgcggtttcg ctaactttgg ttctatcggg gtggtggtgg gggcgttttc
    12001 tgcggttgcg ccacaccgtg cgccggaaat cgcccagctt ggtttacgcg cgctggcggc
    12061 ggcgacactt tctaatctga tgagtgccac cattgccggg ttctttattg gtttagcgta
    12121 gtgtgcaatt tatgagccgg attagatgcg gtcaagcgtc gaatccggca acattgttac
    12181 accattggca ctaatgacag cgcgttatcg gcagacaggg tagggttgtt taagctgccg
    12241 ctggcgcgtg aaattgccgc gcaggccatc gcaaaacggg cgctgtcacg gaaattgttt
    12301 ccctccagaa agctatatac cagacccgcc ataaaaccat cgtcagcacc aaaactgtct
    12361 accgtcgtat gcgctggcac agtcagcaaa aattgttcac catccttttc gctgcaataa
    12421 actgactcat cgggcaaata aacaaacagt tgctgaacac cttgctgatg caatgcattc
    12481 actgcggcat tacggtcagc atcgctggtg atcgcctgtc cccataaaat ttccagctcc
    12541 ggtaaagtgg gtttcagggt gtgaatatgc gccagccagt gtttgatttt gcccgctttg
    12601 aattctgaaa cggtatcgac aaacaccggg atctcatcag cgagggtaaa gacccattcc
    12661 agcgcctcgg ctgtcaggtt gcaatcaacc agtaccacgc ccgcatgacg aagtaaatcg
    12721 cgcgacccgt tcagtagttg cggtgtcaac tgttccagca gatgggtatc gttaatcgcc
    12781 agcacggttt gatcgtctcg attggcaatt gccagatacg tcgatgtgct ttgaccatgc
    12841 aaacgaacgc agccggagac attcacgccg gcacggcgcg tttcttccag gagcatttcg
    12901 ccataaaagt catcgccaat cactgaaagc aaatggacgt cacggcctaa cagcgccaga
    12961 ttgtgggcga tattgcgtcc aacgccgcct gccgaacaat gaattgtacc gggatgagaa
    13021 gccgcttgcg ggtaatggat atccgccatc ccgcgaatat ccatattgat tgtccccacc
    13081 actacgcagt attcctgctc ggtgagaatg taacctttgc ctttaatccg gcctttgcgc
    13141 attaaatcca taatatgcgc cgcaacgcgc gaacgactga tttgcagcat gtccgcaatt
    13201 tcgttctgct gaatcagcgg gttacgccgt aaaattgcaa ggatctcctt ttcccggtta
    13261 ttcatcttca ggctaccgct ttttcagttt gttgcgcttt cagccaggcg atctcttctg
    13321 cccagatatc cgggttgatg gtttcaagga tcagcggaat gccgtcgaaa cggtcgttct
    13381 gcataatcca gcggaacgca tcatgaccga tattgccttc accgaggcta tgatggcggt
    13441 caacgcggct gccaaaggta cttttcgcat cgttaaggtg catcccgcgc agatacttaa
    13501 agccgacaat acgggcaaaa tcagcgaacg ttttttcgca ttcggctgga gtacgcaaat
    13561 catacccggc agcgaaagca tggcaggtat caatgcagac gccgacgcgg gatttatctt
    13621 ccacgccgtc gataatcgcc gcgagatgtt cgaatttaaa ccctaagtta ctgccctgac
    13681 cggcggtgtt ttcaatcacc gctgttacac cttgagtttt atccagcgca atgttgatgg
    13741 attcggcaat acgcgcaagg caatcctctt ctgaaatctg catcagatgg ctgccagggt
    13801 ggaagttgag caaagaaagc cccagttgtt cgcaacgctg catttcatct ataaaggcat
    13861 cgcgcgattt ttccagagct tcagtgaccg gatgtccgag gttgatcaga taactgtcgt
    13921 ggggaagaat ttgcgccgat gtgtagtgat atttttcaca ggcggctttg aattcatcga
    13981 tggtttgcgt cgtgagcggt gcggcacgcc actgacgttg gtttttggtg aacaaggcaa
    14041 acgcggttgc gtcgatttcg gcggcgcgaa ttgcggcatt tgccagaccg ccagcagcac
    14101 taacgtgcgc tccaatgtat ttcatgcgag gactcctgtt aaacccgctg gaggaaaacg
    14161 gtaatgatag cgggttaaca ggagcaagaa gtagtggttt atgcgatgac gctttgaatc
    14221 acatagttaa tcgcaccacc accaacaatc agccaggcaa acagtaccag tgccatcagc
    14281 agaggtttcg ccccagcttt tttcagcgcg ctgacgtgag tggtcagacc cagcgccgcc
    14341 atcgccattg ccagcaggaa ggtatccagc gttaccagca tgttcaccac gctctgcggt
    14401 aacaggtgga acgagttaaa gatggcaact acgatgaaca agatggcaaa ccacggaata
    14461 gtgattttgc ttttctcgcc gctgttcgcc ccagacagct gtttaacacg cgccgccagc
    14521 aggatgagga acggagccag catcatcacg cgcagcattt tggaaataac tgctgcgttt
    14581 tctgcatccg ggctgatggc atgacctgcc gccaccacct gcgccacttc gtgcacagta
    14641 gaaccaatgt agataccgaa agtttccgga ctaaaccatt gagacatcag cggatatatc
    14701 gccgggtaga ggaaaatcgc gacggtcccg aagataacaa cggttgcaac agccacggtt
    14761 actttactgg cttccgcttt cactaccggc tcagtcgcca gtaccgcggc agcaccacag
    14821 atactgctac cggcaccgat caaccaactg gtgtgcttat ccagaccaaa cactttctgc
    14881 cccaggaagc aagccagcag gaaggtactg gacagcgtca gcacgtcaat gatgatccca
    14941 ctgataccga catcggcaat ttgcgagaac gtcagacgga agccataaag aatgataccc
    15001 agacgtaata aatattgctt ggcaaacagc acaccaccgt cacagctttt ccagatgtgc
    15061 ggatagatgg tgttgcctaa aaccatcccc aacaagattg cgagggtgag ggcactaaac
    15121 ccggcacccg caaccgcggg aatggaacca ccccacaggg cgaccccggt gataactgca
    15181 ctcagggcta accccggaat aaaatgccac agtgtacgat gttgtttctg taaggtgata
    15241 ttcgtcataa ccctctcctt taaccgggca taaggttacg actaactggt ttaaaaataa
    15301 aattgattat atatttataa ttaatcttta taagtggtaa gcgactatgc acatcaccct
    15361 ccggcagttg gaagtttttg cagaagtatt gaaaagtgga tcaaccaccc aggcgtcggt
    15421 gatgctggcg ttgtcgcaat cagcagtgag cgcagccttg actgacctgg aagggcagct
    15481 tggcgttcaa ctgtttgatc gcgtggggaa aagactggtt gttaatgaac acgggcggct
    15541 gctctatccg cgagcgttgg cactgcttga acaggcggtt gaaatcgaac aactgtttcg
    15601 cgaagacaac ggcgcgattc gtatctatgc cagtagtacc atcggtaact acattctgcc
    15661 tgcagttatc gcccgttatc gccatgatta tccgcagttg ccgattgaac ttagcgttgg
    15721 gaacagccag gacgtgatgc aagcggtgct ggatttccgc gttgatattg gctttattga
    15781 aggaccgtgc cacagcactg aaatcatttc tgaaccgtgg ctggaagacg agctggtggt
    15841 tttcgccgcg ccgacttcgc cgttggctcg tggtccggtc accttagaac agctggccgc
    15901 tgcgccgtgg atcctgcgtg aacgcggttc cggcacgcgg gagattgtcg attatctgtt
    15961 gctgtcacat ttaccgaagt ttgagatggc gatggaatta ggtaactccg aggcaatcaa
    16021 acatgcggtg cgtcatgggt tgggaattag ttgcctgtcg cgacgtgtga ttgaagatca
    16081 attgcaggca ggcacattaa gtgaagttgc ggtccctctg ccgcgcctga tgcgtacgtt
    16141 gtggcgtata catcatcggc aaaaacacct ttccaacgcg ctacggcgct ttctggacta
    16201 ttgcgatccc gcaaatgtgc cgcgttaagt tgctgtacaa gaacatgctg gtgctgtgtc
    16261 gatttcgtga cgcagcgcct tcagcatgca ttcgccagaa aagagattgg ctgctttact
    16321 tataatccct gggcgatcat gaaggtgtct tataaccgtg tatttctgcc ggaaggattg
    16381 ccaatcgtct gctacaatcg cgccatcatt tttaagatgg atagcatttt tgtatgggtt
    16441 ccgaaactaa aactacagaa gcgccgggct tacgccgtga attaaaggcg cgtcacctga
    16501 cgatgattgc cattggcggt tccatcggta caggtctttt tgttgcctct ggcgcaacga
    16561 tttctcaggc aggtccgggc ggggcattgc tctcgtatat gctgattggc ctgatggttt
    16621 acttcctgat gaccagtctc ggtgaattgg ctgcatatat gccggtttcc ggttcgtttg
    16681 ccacttacgg tcagaactat gttgaagaag gctttggctt cgcgctgggc tggaactact
    16741 ggtacaactg ggcggtgact attgccgttg acctggttgc agcacagctg gtcatgagct
    16801 ggtggttccc ggatacaccg ggctggatct ggagtgcgtt gttcctcggc gttatcttcc
    16861 tgttgaacta catctctgtt cgcggctttg gtgaagcgga atactggttc tcactgatca
    16921 aagtcacgac agttattgtc tttatcatcg ttggcgtgct gatgattatc ggtatcttca
    16981 aaggcgcgca gcctgcgggc tggagcaact ggacaatcgg cgaagcgccg tttgctggtg
    17041 gttttgcggc gatgatcggc gtagctatga ttgtcggctt ctctttccag ggaaccgagc
    17101 tgatcggtat tgctgcaggc gagtccgaag atccggcgaa aaacattcca cgcgcggtac
    17161 gtcaggtatt ctggcgaatc ctgttgttct atgtgttagc gatcctgatt atcagcctga
    17221 ttattccgta taccgatccg agcctgctgc gtaacgatgt taaagacatc agcgtcagtc
    17281 cgttcaccct ggtgttccag cacgcgggtc tgctctctgc ggcggcggtg atgaacgcag
    17341 ttattctgac ggcggtgctg tcagcaggta actccggtat gtatgcgtct actcgtatgc
    17401 tgtacaccct ggcgtgtgac ggtaaagcgc cgcgcatttt cgctaaattg tcgcgtggtg
    17461 gcgtgccgcg taatgcgctg tatgcgacga cggtgattgc cggtttgtgc ttcctgacct
    17521 ccatgtttgg caaccagacg gtatacctgt ggctgctgaa cacctccggg atgacgggtt
    17581 ttatcgcctg gctggggatt gccattagcc actatcgctt ccgtcgcggt tacgtattgc
    17641 agggacacga cattaacgat ctgccgtacc gttcaggttt cttcccactg gggccgatct
    17701 tcgcattcat tctgtgtctg attatcactt tgggccagaa ctacgaagcg ttcctgaaag
    17761 ataccattga ctggggcggc gtagcggcaa cgtatattgg tatcccgctg ttcctgatta
    17821 tttggttcgg ctacaagctg attaaaggaa ctcacttcgt acgctacagc gaaatgaagt
    17881 tcccgcagaa cgataagaaa taagttttct cccttccttg ctaagccctc tcaaccgaga
    17941 gggctttttc aattccattt ccctgacaaa tcatgcggat ataaaattta acatttggat
    18001 tgataattgt tatcgtttgc attatcgtta cgacgcaatc aaaaaaggct gacaaatcag
    18061 aggctgttcc ggctttctgg gatgatcacc tgcatacaaa taagtccacc gcgatgctgc
    18121 cgtacgcaag gggacgtaaa gaagatgtga gcgataccca ttttattttc gtagttacct
    18181 catggagata tggaatgttt aggttgaacc ctttcgtacg ggtcgggctg tgtttgtccg
    18241 ctatttcttg tgcatggcct gtgttagcgg tcgatgatga tggcgaaacg atggttgtca
    18301 ctgcatcttc cgtggaacaa aatcttaaag atgcacccgc cagtatcagc gtcattaccc
    18361 aggaagacct gcagcgaaaa ccggtacaga atctgaagga tgtcctcaaa gaagtgcctg
    18421 gcgtacaact gacgaacgaa ggggataacc gtaagggcgt tagtattcgt ggtctggaca
    18481 gcagctacac cctgattctt gtcgacggta aacgcgttaa ctcccgcaat gccgtcttcc
    18541 gccacaatga tttcgatctg aactggatcc cggtcgattc catcgaacgt attgaagtgg
    18601 ttcgtggccc gatgtcgtcg ctatacggct ccgatgcgct cggcggtgta gtgaatatca
    18661 tcaccaaaaa aatcggtcag aaatggtcgg gtaccgttac cgtcgatacc accattcagg
    18721 aacatcgcga tcgcggtgat acctataacg gtcagttctt taccagtgga ccattaattg
    18781 atggtgtgct gggaatgaaa gcttacggca gcctggcaaa acgtgaaaag gatgacccgc
    18841 aaaactcaac gaccaccgat accggagaaa cgccgcgtat tgaaggattc tccagccgcg
    18901 acggcaatgt cgaatttgcc tggacaccga atcaaaatca cgattttact gccggatacg
    18961 gtttcgaccg tcaggatcgt gattccgact cgctggacaa aaaccgcctg gaacgccaga
    19021 actactgcgt cagccataat gggcgttggg attacggcac cagcgaactg aaatcctacg
    19081 gtgagaaagt cgagaacaaa aaccctggca acagcagccc gataacttcc gaaagcaata
    19141 cggtcgacgg caaatacacg ttgccgctga cggcgattaa tcagtttctc acggttggcg
    19201 gtgaatggcg tcacgacaaa cttagcgatg cggtgaacct gaccggggga accagctcca
    19261 aaacgtctgc cagccagtac gcgctgtttg tggaagatga atggcggatc ttcgagccgc
    19321 tggcgctgac gaccggcgtg cgtatggacg atcacgaaac ctacggtgaa cactggagtc
    19381 cgcgtgccta cctggtttat aacgccaccg acaccgtaac ggtgaaaggg ggctgggcga
    19441 cggcatttaa agcaccttct ctgttgcaac ttagccctga ctggacgagc aattcctgcc
    19501 gtggcgcatg taagattgtg ggtagcccgg atctgaaacc agaaaccagc gaaagttggg
    19561 agctggggct ttactacatg ggtgaagaag gctggctgga agggggtgaa tccagcgtta
    19621 tcgttttccg taacgatgtg aaagatcgta tcagcatcag ccgtacgtct gacgtcaacg
    19681 ctgcaccggg ctaccaaaac tttgttggtt ttgagacggg cgctaacgga cggcgcatac
    19741 cggtatttag ctactacagc gttaacaaag ctcgtattca gggcgtggaa accgaactga
    19801 aaattccgtt caacgatgaa tggaaactgt cgatcaacta cacctacaac gatggtcgtg
    19861 atgtcagcaa cggcgaaaac aaaccgctat ccgatctgcc gttccatact gctaacggta
    19921 cgctggactg gaaaccgctg gcgctggaag actggtcatt ctatgtttct ggtcactata
    19981 ccgggcagaa acgcgccgac agcgcgacgg ctaaaacacc gggcggttat accatctgga
    20041 ataccggcgc ggcctggcag gtgactaaag acgtcaaact gcgcgcaggc gtgctgaacc
    20101 ttggcgacaa ggatctcagt cgtgacgact acagctataa cgaagacgga cgtcgttact
    20161 ttatggcagt ggattatcgc ttctgatgag aagatgcccg gcgaaccggg cggactttca
    20221 cttcagtaaa tactgcgcat ggaagcgcag gtgatcctct ataaaagagg cgatgaagta
    20281 gtaactgtga tcatatcccg gctgaatacg cagcgtcatc ggccacgctt tctggcgcgc
    20341 ggcttctgcc aataccgcag gttgcaactg gtcggcaaga aactgatcat tatcgccctg
    20401 atcgataagc gtcgggatcg catcctgcgc gttactggca tacatcagcg cgcaactatc
    20461 ccattccagc catgcatttt tgtcctcacc taaataggtg ctaaacgctt tgattcccca
    20521 cggggcgctg cacggattca caatgggcgc aaaggccgaa acgctggtgt atttacccgg
    20581 atttttcagc gccataatca gcgcaccgtg accacccatt gagtgaccgc taatggcgca
    20641 gcggtcgctg acattaaatt gcgactgaac cagcgccggt aattcatcgc gcagataatc
    20701 atacatccgg taatacgtcg cccacggcgg ttgcgtggca ttaagataaa agcctgcgcc
    20761 ctggcctaaa tcgtagccat cgtcgttggc aaccttttcg ccgcgcgggc tggtgtctgg
    20821 catcaccagt acaatcccca gttccgccgc tacccgctgg gcacccgcct tggtggtgaa
    20881 gttctcgtca ttgcaggtta atccggaaag ccagtacagc actggtggcg gggtgtgatc
    20941 acgagggggt gggagaaaga tactgaacgt catcgggcag tttaaggtac tggagtcgtg
    21001 tcgccagcgt tgctgccagc cttcaaaaca gcggtgctct tcgagcattt ccatgcgtgg
    21061 ctccttgttg tgttgtttgc aatttcttca tcataccgtt tattcaatgt gctgtgagta
    21121 actttcactt ccgtatttgc ataacgatgt tttaacatct gctgatgaaa ggcagcggca
    21181 attacaataa ttatcgctgt gaatactgga ttatgtgcgc cgcctcacgc acaataatca
    21241 agctgtaaat cagcttaata actttgcccc cacgcagggc ggaggcgtca cacctgcagg
    21301 agaaatcata aatgccatca ctcagtaaag aagcggccct ggttcatgaa gcgttagttg
    21361 cgagaggact ggaaacaccg ctgcgcccgc ccgtgcatga aatggataac gaaacgcgca
    21421 aaagccttat tgctggtcat atgaccgaaa tcatgcagct gctgaatctc gacctagctg
    21481 atgacagttt gatggaaacg ccgcatcgca tcgctaaaat gtatgtcgat gaaattttct
    21541 ccggtctgga ttacgccaat ttcccgaaaa tcaccctcat tgaaaacaaa atgaaggtcg
    21601 atgaaatggt caccgtgcgc gatatcactc tgaccagtac ctgtgaacac cattttgtta
    21661 ccatcgatgg caaagcgacg gtggcttata tcccgaaaga ttcggtgatc ggtctgtcaa
    21721 aaattaaccg tatcgtgcag ttctttgccc agcgtccgca ggtgcaggaa cgtctgacgc
    21781 agcaaattct tattgcgcta caaacgctgc tgggcaccaa taacgtggct gtctcgatcg
    21841 acgcggtgca ttactgcgtg aaggcgcgtg gcatccgcga tgcaaccagt gccacgacaa
    21901 cgacctctct tggtggattg ttcaaatcca gtcagaatac gcgccacgag tttctgcgcg
    21961 ctgtgcgcca tcacaactga ttaaaaggca ggaaccatgg agcgcaacgt cacgctcgat
    22021 tttgttcgcg gcgtcgccat tctggggatc ctgctattaa acatcagcgc ctttgggcta
    22081 ccaaaggcgg cttatctcaa tcccgcctgg tacgatgcta ttacgccgca ggatgcatgg
    22141 acctgggcat ttctcgatct catcggccag gtgaaattcc tcacgctttt tgcgctgctg
    22201 tttggtgctg gcctgcaaat gttgctgccc cgtggcagac gctggatcca gtcacggtta
    22261 acgctgttag tcttgttggg ctttattcac ggtttattgt tctgggacgg cgatattctg
    22321 ctggcttacg ggctggtggg cttaatctgc tggtggctgg tgcgcgatgc gccatcggta
    22381 aaaagcctgt ttaataccgg cgtcatgctt tatctggtgg ggcttggcgt tttgctgtta
    22441 ttggggctga tttccgacag ccagaccagc cgcgcctgga cgccggatgc atcggctatt
    22501 ttgtatgaaa aatactggaa gcttcacggc ggcgttgaag cgatcagtaa tcgtgccgat
    22561 ggtgttggca acagtttact ggcactgggc gcacagtatg gctggcaact ggcagggatg
    22621 atgctcattg gtgcggcgct gatgcgcagc ggctggctga aagggcagtt cagcttacgt
    22681 cactatcgtc gtactggttt tgtgctggtg gcgattgggg tgaccattaa ccttcctgcc
    22741 atcgccctgc aatggcagct ggactgggcg tatcgctggt gtgccttctt acttcaaatg
    22801 ccgcgggaac tgagtgcgcc gtttcaggcg attggctatg cgtcgctgtt ttatggcttc
    22861 tggccgcaat tgagccgctt taagctggtg cttgcgatcg cctgcgtcgg acggatggcg
    22921 ctgaccaact atctattgca aacgctgatt tgtaccacgc ttttttacca cctcggtttg
    22981 tttatgcatt ttgaccgcct ggagctgctg gcgtttgtta ttccggtatg gctggcgaat
    23041 atcctcttct ctgttatctg gctgcgttac ttccgccagg ggccggtgga atggctctgg
    23101 cgtcagttaa ctttgcgtgc tgccggactg gcaatatcta aaacatcaag ataacgatct
    23161 ggatcacatt cgttaacaaa acggctgtaa ccgtttccat tgctgtgact cgattcacga
    23221 agtcctgtat tcagtgctga caaaatagcc gccagcaagc agtcatttac tgcaatctca
    23281 taacaggtag tgaatatgat caccattcgt gatgtagcgc gtcaggctgg cgtctctgtg
    23341 gcaacggttt cccgggtgct caataacagc acgctggtca gtgccgacac gcgtgaagca
    23401 gtaatgaaag ccgtgagtga gctggattat cggccaaaca ccaatgccca ggcgctggca
    23461 actcaggtta gcgacaccat tggcgtggtg gtgatggacg tttctgatgc gtttttcggc
    23521 gcgctggtaa aagcggtgga tctggtcgcc cagcagcatc agaaatacgt gctaatcggc
    23581 aatagctatc atgaagcgga aaaagagcgt cacgccattg aggtgttaat tcgccagcgt
    23641 tgtaatgcgt tgattgttca ctcaaaagca ttgagtgacg atgaactggc acaatttatg
    23701 gataacattc ccggtatggt gttaatcaac cgcgttgtgc cggggtacgc ccatcgttgc
    23761 gtttgcctgg ataatctcag cggtgcccga atggcgacgc gcatgttgct gaataacggt
    23821 catcaacgta ttggttatct ttcttccagc cacggcattg aagatgacgc catgcgtaaa
    23881 gcaggctgga tgagtgcgtt gaaagagcag gatattattc cgccggaaag ctggattggc
    23941 actggtacgc cggacatgcc gggcggtgag gcggcgatgg ttgaactgct ggggcgcaat
    24001 ctacaactta ccgctgtatt tgcttataac gacaatatgg ccgctggcgc actgacagca
    24061 ttaaaagata atggcattgc gattccgtta catctctcaa tcatcggttt cgatgatatt
    24121 cccatcgccc gttacaccga cccgcaatta acgaccgtgc gttatcccat tgcttcaatg
    24181 gctaaattag ccaccgaact ggccttgcag ggggcagcag gcaatattga tcctcgtgcc
    24241 gccactgttt tatgccgacg ttagtgcgtc gccattctgt cgcaacgcgc cagaatgcgg
    24301 cggcgatcac taactcaaca aatcaggcga tgtaaccgct ttcaatctgt gagtgatttc
    24361 acagtatctt aacaatgtga tagctatgat tgcaccgttt taatgttgta acccgtatgt
    24421 aacagtgaat aatcactttc accgaggtaa tagcgtcata acaacaatta aagccgtttt
    24481 ctggagcgtt accgggcatg gaagaacgaa ttttaaaaag tgagcttcgg cgttcagtaa
    24541 cacttcatta actctactgc cccgccgagc atttatctca agcactaccc tgcataagaa
    24601 aaaccggaga taccatgaat aagaaggtgt taaccctgtc tgctgtgatg gccagcatgt
    24661 tattcggtgc cgctgcacac gctgctgata ctcgcattgg tgtaacaatc tataagtacg
    24721 acgataactt tatgtctgta gtgcgcaagg ctattgagca agatgcgaaa gccgcgccag
    24781 atgttcagct gctgatgaat gattctcaga atgaccagtc caagcagaac gatcagatcg
    24841 acgtattgct ggcgaaaggg gtgaaggcac tggcaatcaa cctggttgac ccggcagctg
    24901 cgggtacggt gattgagaaa gcgcgtgggc aaaacgtgcc ggtggttttc ttcaacaaag
    24961 aaccgtctcg taaggcgctg gatagctacg acaaagccta ctacgttggc actgactcca
    25021 aagagtccgg cattattcag ggcgatttga ttgctaaaca ctgggcggcg aatcagggtt
    25081 gggatctgaa caaagacggt cagattcagt tcgtactgct gaaaggtgaa ccgggccatc
    25141 cggatgcaga agcacgtacc acttacgtga ttaaagaatt gaacgataaa ggcatcaaaa
    25201 ctgaacagtt acagttagat accgcaatgt gggacaccgc tcaggcgaaa gataagatgg
    25261 acgcctggct gtctggcccg aacgccaaca aaatcgaagt ggttatcgcc aacaacgatg
    25321 cgatggcaat gggcgcggta gaagcactga aagcacacaa caagtccagc attccggtgt
    25381 ttggcgtcga tgcgctgcca gaagcgctgg cgctggtgaa atccggtgca ctggcgggca
    25441 cagtactgaa cgatgctaac aaccaggcga aagcgacctt tgatctggcg aaaaacctgg
    25501 ccgatggcaa aggtgcggct gatggcacca actggaaaat cgacaacaaa gtggtccgcg
    25561 taccttatgt tggcgtagat aaagacaact tggctgaatt cagcaagaaa taagactgat
    25621 tgggcgcagt cgtttactgc gcccttattg tatggccaac aaggtaaaat tatatggtca
    25681 gctcaacgac tccgtcttcc ggggaatact tgttggaaat gagcggtatc aacaagtcct
    25741 ttcctggtgt taaggcactt gataacgtta atttaaaagt ccggccacat tctatccatg
    25801 cattaatggg ggaaaacggt gcaggaaaat cgacattatt aaaatgcctg tttggtattt
    25861 atcaaaaaga ctccggcacc attttattcc agggtaaaga gatcgatttc cattccgcca
    25921 aagaggcact ggaaaatggt atttcgatgg tacaccagga gttaaacctg gtattacaac
    25981 gttcggtgat ggataacatg tggctggggc gatatcccac caaaggcatg tttgtcgatc
    26041 aggacaaaat gtaccgcgaa accaaagcga tttttgatga actggatatt gatatcgatc
    26101 cgcgtgcgcg cgtcggcaca ttatccgttt cgcaaatgca gatgatcgaa atcgccaaag
    26161 cgttttccta taacgcgaaa attgtgatta tggatgaacc gacttcttcg ttaaccgaaa
    26221 aagaggtcaa tcatctgttc actattattc gtaaattaaa agagcgcggc tgcggtattg
    26281 tttatatctc gcataaaatg gaagaaatct tccagttatg tgatgaagtt accgtattgc
    26341 gcgacggtca gtggatcgcc accgaaccgc tggcaggact gacgatggac aagatcatcg
    26401 gcatgatggt tgggcgttct cttaatcagc gttttcctga caaagaaaac aagccgggcg
    26461 aagtcatcct cgaggtgcgt aacctaacgt cactgcgcca gccgtcgatt cgcgatgtct
    26521 cgtttgatct gcataaaggg gagatcctcg gtattgccgg gctggtgggg gcgaaacgta
    26581 ccgatattgt tgagacgtta tttggtattc gcgagaaatc ggctggcacc attacgttgc
    26641 acggcaaaca gatcaataac cataatgcca acgaagccat aaaccacgga tttgcactgg
    26701 taactgagga gcgccgctca acgggaattt atgcctatct ggatattggt tttaactcgt
    26761 taatttccaa tattcgcaac tacaaaaata aagttggttt actggataac tcgcggatga
    26821 aaagcgatac ccagtgggtg attgattcga tgcgggtaaa aacgccgggt catcggacgc
    26881 aaattggttc gctctccggt ggtaatcagc aaaaggtgat tattggtcgc tggctactaa
    26941 cgcaaccaga aatattaatg ctcgatgaac cgacgcgcgg tattgatgtc ggggcgaagt
    27001 ttgaaattta tcagttaatt gccgaactgg cgaagaaagg caaggggatt attattatct
    27061 cctctgaaat gcctgagttg ttagggataa cagaccgtat tctggtcatg agcaatggtc
    27121 tcgtttccgg aattgtcgat acaaaaacaa caacgcaaaa cgaaattctg cgtcttgcgt
    27181 ctttgcacct ttaagattag gggcttccca tgagtgcgtt aaataagaaa agttttctta
    27241 cttacctgaa agagggcggt atttacgtcg ttcttttagt tttgctggcg attattattt
    27301 tccaggaccc aacattttta agtctgttga acttaagtaa tattctcacc cagtcatcgg
    27361 tgcgtattat tatcgcgctc ggtgtggcag ggttaattgt cacccagggg accgatcttt
    27421 ctgctggtcg tcaggtaggg ctggcggcag tggtggctgc gacattattg cagtccatgg
    27481 ataacgccaa caaagtgttc ccggaaatgg cgacgatgcc gattgcgctg gttattctga
    27541 ttgtctgtgc cattggtgcg gtgatcggtt tgatcaacgg tctgattatc gcttatctca
    27601 acgtgacgcc gttcattacc acgctcggca cgatgatcat cgtctatggc atcaactcgc
    27661 tctattacga ctttgtcggg gcgtcgccaa tttctggttt tgacagtggc ttctctacct
    27721 ttgctcaggg ctttgtcgcg ctggggagtt tccgtctctc ttacatcacc ttctacgcgt
    27781 tgattgcggt ggcgttcgtc tgggtgttgt ggaacaaaac ccgcttcggt aagaacattt
    27841 ttgccattgg cggtaacccg gaagcggcaa aagtatctgg tgtcaacgtc ggcctgaacc
    27901 tgctgatgat ctacgcgttg tctggcgtgt tctatgcctt tggcgggatg ttagaagccg
    27961 gacgtatcgg ctctgccacc aacaacctcg gctttatgta tgagctggat gctatcgcgg
    28021 cgtgcgtggt aggcggtgta tcgttcagcg gcggtgtggg gacggtgatt ggcgtggtga
    28081 ccggggtaat tatttttacc gtcatcaact atggcctgac gtatatcggc gtaaacccat
    28141 actggcagta catcatcaaa ggggcgatta ttatcttcgc cgtagcgctg gattcactga
    28201 aatacgcgcg taagaagtga tgtgattgcg gatattgccg gatgcgtctc tggttttaca
    28261 acgttaccgg gtgttctttc tcgcctttct taaacttcac ttccccgagc tcaatacaac
    28321 ccaccgggca gacgtgacca caaagcagac aacccacaca tttctcggta ttacaatgcg
    28381 gtgtgcgggt tttctcgctc cattccatcg cctggtgacc gccgtcgtaa caggaaatat
    28441 aacagcgtcc acagccaaca catttgtcaa gattgatacg gggatagaca atataactgc
    28501 ggtctaaatc ttccgccggg acaatattgt tattcgccag acctaccatc tcctgcaacg
    28561 aatcaaatcc ctgatcggcg agataatgcg ataacccgct cgccatatct tctacgatgc
    28621 gatagccgta ctgcataatg ccggtggtga cctgtaacgt tgctgcgccg agcaataaaa
    28681 actccgccgc atcttcccag gtttcaatgc cgccgatacc gctgattggg aaatcgcgca
    28741 gttctggatg ggtgcgcatt tgctgaatga agcgcagggc gatcggtttt accgctttac
    28801 cggaatatcc ggaaatactc gatttaccgt taacgatcgg cataccgatt ttctgattaa
    28861 gatcgatatt ggtgatggat ttaacggtgt taattgccgc aatgccatct gcgccgccgc
    28921 gcttcgccgc cagcgccact tcgcacatat caccgatatt cggcgtcatc ttcgccagca
    28981 ttggcagcgt ggaaccccgt ttcactgccc gacaatattt ttttaccagc tccgggcttt
    29041 gcccgacatc gctacccatc gcatgagaag tcatttgcgg acaggagaag ttacactcga
    29101 tcatatccgc gccagcttct tgcaccaggc gcgccagctc ctcccattgc tgctcatttt
    29161 cccccatgat cgaagcgatc aatactttgt ccgggtaatc ttccttcagc cgacgcaggg
    29221 cggtcagatt ctcttccaac ggatgttcag caatctgctc catatttttg aagccgataa
    29281 aaccggtatc ttctttcacc agatgatcaa aacgcggcga gacttcgttg gcgataaaaa
    29341 agccgatcgt tttaaacacc acaccgcccc aacctgtgtc gtaggctttg gcacacatct
    29401 catagcagtt gcctaccggc gaagaagaga ggcagaacgg gttgggaaag ttcacgccgc
    29461 aaaaagtaat cgaaagatct ttcgttaaca tgagcaagct ccctctaata tagtgatgaa
    29521 tcgcctcggc ggcttctttc ccggttttca cggcatagac cacggttttg tcaccctcaa
    29581 caatatcgcc agcagcaaag acttgcggat cgcgggtctg ataattttgt gttttgatgg
    29641 tgttacgctg ctgctctaac tccgcaaagg catccagtct ggcatgctga ccgacggcga
    29701 gaataatttt atctgccgcc atcgtcagtt cgcctgataa ccgtacatgc ttaaacgtga
    29761 ctttattgcc ttccacggct actggcgtga atccatcgat gatcgaaacg cccagctccc
    29821 gggcgctggc aaactctttt tcgcttgccg gaaactcatc taactcttca cgtgctacgc
    29881 aagttaccgc ctgacagccg agaactttca gcgtgctggc tacgtccatc gcgacatcac
    29941 cgccgccgat aattaatgcg ctttgtggaa tgctgatatc gccttgcgcc tgtcgtacac
    30001 gttgcaggaa gtcgacggca atctcaacgt cgctatgctc aaacagcggt agcccggaac
    30061 cgcttgataa ccccacggtg accagtaccg cgcggttttc cgctttcagt tgctccaggg
    30121 tgagtgtttt accgatttcg ttattgcact taatggtcac gcccattttt tcaatacggg
    30181 cgatctctgc atccagcact gattgcggta accggaattg cggaataccg ttacgcaacc
    30241 agccaccggg gtgcgcttct ttctcataaa tcgtgacgtc ataaccctgg tttgtcagtg
    30301 tcacactggc ctgcaatccg gcaggacctg cgccaataat cgcgactttg ccgagcgttt
    30361 tagtaccggg ctgataaatt tccatcccgg tttgttgttc aaaatcagta acaaaacgct
    30421 gtaagcggcc gatatcaatg ggagcatcaa caccggcacg ggtacaaccg ctttggcata
    30481 atttctccgt cgggcacact ctggcacaaa cggcaccgag ggcattattt tcgcgaattg
    30541 tctcggcagc gcctttaaaa ttacgaaagt agattgagcg aataaatttc cccggatcgg
    30601 tctgtgccgg gcaagcctga ctacagggag cgtcgtgaca taataaacag cgagaggctt
    30661 ctttaatcgc cagtaacggc gtaaaagccg gagtgagttc atccagataa ttttgttgcg
    30721 gcatgtgatc ttccttaatg ctatgagtaa ttatggcgaa cgctaaataa atattgcgtg
    30781 ctgatattaa ggctatgcgc gtggggaaat ttaaactgtg aaaggattca caaatagctg
    30841 cggttcagaa tgttatatcc gcagcttatt gagtgaagta ataattattc tcaataatta
    30901 acagaaaaga gaaaatatta acgatagaaa ataagataaa caatcatgcc gatagataaa
    30961 attccgaagc caatatatgc caccatataa gcgaagaaga gtccgggatt ttttgctcgc
    31021 cagacataca ccggttccgg tgcattttta acccgtttat caatcgcgcc tgcgcgccag
    31081 cctttccatg cttcgcggaa acaaaatatc gggataagga aaaaaacggc aacgacaaaa
    31141 aactgctgta catccataac tactttcctt gtttcttttg taattcaagt aactgttcgg
    31201 gtgtaacggc gggatacccc tgcgcatctt ccggcacctg gtgcatagcc ggaataggga
    31261 ccagcggccc taaaaaacgc ggttcacgtt taaaaatata aaggtcagcc agcgcaccga
    31321 aacgggcggc aaattcgcga atacgtactg acagcatatc tttcggtgac ggtacggcat
    31381 aacactgagc ctgaatcccc atatgcagcg caataaataa tgctcgctca cagtggaaac
    31441 gttgggtgat aataatgaaa tcattagtat cgaaaacttt gcgtgtacgc acgatggaat
    31501 ccagcgtacg aaagcctgcg taatcgagaa caatatctga tgggtcgaca ccagcagcga
    31561 ttaaatcttt gcgcatggtc atcggctcgt tataactttg caatgcgtta tcgccgctca
    31621 gtaatagata atttacctta ccgctgttat aggcattaat cgctccttga atgcggtagc
    31681 gataatactg attaataacg ccggtacgat aatattttgc tgtgccgagc accacgccga
    31741 cctggcggta ggggagatcc tgcaattcgt cgtagatata aggcgcggtt ttccagctca
    31801 tccagcgatc gaggccgagc acagtcaaca gcagcaagcc gatcaggact aacaggctga
    31861 ggaacacgcg ctttaacatg caggagactc gagctaagga aaatttccat caggctactt
    31921 tacccgctgg ttttgcgcaa gaaacggcaa actgtttgcc gggaatttcg acaccaggag
    31981 agggctggca agccggacaa ggcgttcacg ccgcatccgg caccaggctt aagcgagaag
    32041 cacgcggtcg atactgtgac agccgagagc tttcagcgtt gcggaggtgg catcccactg
    32101 aatcaacggc gcatcggctt tttctgccag aaccgcgcgc tggatatccg ggtaatcata
    32161 acccttgaga ttcaacagaa ttaacgcccc ctgcaacggc ggcagagtcg ggttgaatgc
    32221 ggcgttttca gcgtagctgc cgctgaaaat acgaccgtct ttacattcca gcgcgacacc
    32281 gcttggtgac ttactgtaag gcatgtgcga acggtttgcc gctgcaatcg ccgcctgaga
    32341 aagcgcatca ccagtcagcg catagccgtg atcctgctcg tccatcagca gcgttttaat
    32401 ctccagatct ttcggcccaa aggcatccgg cagatagtca cgcagcgcgt gtgcctcgcg
    32461 gcccggcaaa tgaatacgca gatccagacc gctgttcagt tcattcataa actgacggca
    32521 gtgaccacaa ggcgtatagt taacggtgat ggctgcaagc gctttttcac cactcaacca
    32581 ggcgtggctg atcgcgcttt gttcggcatg aacggtttgc tgcattgttg caccaataaa
    32641 ttccatattg gcaccaaaat accaggttcc gctcacaccg cgcgcaattg cgccaacatt
    32701 aaaattcgac aatggcgtac gcgcacaggc cgccgccagc ggaagtagtg cgaatgccag
    32761 cgcgtcttcg tccagccccg ttgcgctctt cagcgatgag acttgctccc cggtcaacaa
    32821 agcggggaag tacttgtctg ccagaatagg ttccagtgca gattgcaagt tatccgcaag
    32881 ttgggcaaaa gcggtttgaa aacgtggatg catggcgttg cctcataatc atgtaatggg
    32941 ataacagttt acgaacccgt tatggcttta tatgtgatct gaatctcatt aattatgcaa
    33001 cacgtgaaac ttacatcaaa aatgcgcgac gtatcgcaaa ttttagccca ttactgccag
    33061 aataatcggg aaaaggaacg gtgcgatcag cgaagtaatt atcccgcata acaccagcgc
    33121 tagcgaacta aatgcacctt cctgataatc cagctcggcg cagcgcgccg taccgagggc
    33181 gtgcgaggca gtccccattg ccagaccgcg cgcaactttg gtacgaatac gcatcgcatt
    33241 aagcaatgta tggccaaata ccgcgccgag gatgccgacg aaaatcacgc aaactgcgct
    33301 gattgccgga ataccgccaa tactgccgcc aaccgacatt gcaataggcg tggtgactga
    33361 tttcggcagg attgacgcgg cgatttccgg tgaagcgccc atcaacaatg ccacggaagt
    33421 ccccgtcacc atcgcaacca cgctgccgat aaaacagatg gtgatgatcg atttccagcg
    33481 cgcgcggatc tggtgtagct gctcatataa aggataggcc agcgcgacga ccgccggttg
    33541 cagcagatcg tttagcactt cgctaccttt aaagtaactg tcgtaagaga tgccggtcag
    33601 cattaaaaaa ggaatgatca ccaccattgc taccagcaac gggttaagca ggggaaactt
    33661 ataccgtgcc gccagtttgc gggcggcaaa aaagacaatc aacgtcagcg gtaatgacca
    33721 ccagatattc gccatcattc ttctgatcct ttctgaccta cgactttacg ttcaccgtgt
    33781 accagttgtg aactccagct caccaccaga aaaaccacca gcgtactgac agcacaggaa
    33841 accactaccg ggccaaactg cgcgcggagc aaatcaaaat attgcatgac gcctacgcca
    33901 atcggcacaa acaatagcgc catatagcga atcagtacgt agcaccccgg attgacccat
    33961 ttcgccggaa gaatttgcaa ggccagcagg acaaacagga tcagcatccc gatgatgctg
    34021 cccggaatgg ttaccggtag cagggaagca atgaaaatgc ctgcatacag gcaggcataa
    34081 atcaggacga aagcgcgtaa atattgccag ataatgttca gtgtcttact cataatcaac
    34141 accccagatg agatggattc atcatacaat taattcgtaa aacgtgctac cgatcacatt
    34201 aagaaatcag cgcttatttt ttcgctaccc ggtattacgc aatgagcagc gaactcaggc
    34261 atacatcaag catagagagg gattgcagtt tgcagcaatc cctctttgca gacctttaat
    34321 ccgctgacca taggcgctaa catagcgcct catttttttg cgggtgatga tatacgtgtg
    34381 ttactggcac cgatggaggg agtgcttgac tctctggtgc gtgaattgct gaccgaagtt
    34441 aacgactacg atctgtgcat caccgagttt gtccgcgtgg tggatcaact gctgccggta
    34501 aaagtctttc atcgcatttg ccctgagcta caaaacgcca gccggacacc atctggtacg
    34561 ctggtgcgcg tgcagttgtt aggtcagttc ccacaatggc tggcagagaa cgccgcccgt
    34621 gcggtggagt taggttcctg gggcgtggat ctcaattgcg gctgcccgtc gaaaacggtt
    34681 aacggtagcg gcggcggggc gacgttactc aaagatcctg aactcatcta ccagggggca
    34741 aaagcgatgc gtgaagctgt accggcgcat ttgcctgtca gcgtgaaagt gcgtctgggc
    34801 tgggacagcg gtgagaagaa atttgaaatc gccgatgcgg ttcaacaggc tggcgctacg
    34861 gagctggtgg tgcatgggcg gacgaaagag cagggttacc gcgcggagca tattgactgg
    34921 caggcgattg gcgagattcg ccagcggctg aatattccgg tgattgccaa cggtgaaatc
    34981 tgggactggc agagcgcgca acaatgcatg gcgatcagcg gctgcgacgc agtgatgatt
    35041 ggtcgcgggg cgctcaatat tcccaacctg agccgggtgg taaaatataa cgaaccgcga
    35101 atgccgtggc cggaggtggt tgctttgctg caaaaatata cccgtctgga aaagcagggc
    35161 gataccgggt tatatcacgt tgcgcggatt aaacagtggt tgagttattt gcgtaaagaa
    35221 tacgatgaag caacggaatt atttcagcat gttcg
//
LOCUS       DAJTYG010000034        35191 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTYG010000034 DAJTYG010000000
VERSION     DAJTYG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.034"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            607..2892
                     /gene="nrdA"
                     /locus_tag="OO568_001773"
     CDS             607..2892
                     /gene="nrdA"
                     /locus_tag="OO568_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6641149.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3044..4174
                     /gene="nrdB"
                     /locus_tag="OO568_001774"
     CDS             3044..4174
                     /gene="nrdB"
                     /locus_tag="OO568_001774"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6641150.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4174..4428
                     /gene="yfaE"
                     /locus_tag="OO568_001775"
     CDS             4174..4428
                     /gene="yfaE"
                     /locus_tag="OO568_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6641151.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4482..5132)
                     /gene="inaA"
                     /locus_tag="OO568_001776"
     CDS             complement(4482..5132)
                     /gene="inaA"
                     /locus_tag="OO568_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6641152.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSRVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5296..5538
                     /locus_tag="OO568_001777"
                     /pseudo
     CDS             5296..5538
                     /locus_tag="OO568_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5580..6656)
                     /gene="glpQ"
                     /locus_tag="OO568_001778"
     CDS             complement(5580..6656)
                     /gene="glpQ"
                     /locus_tag="OO568_001778"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6641153.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6661..8019)
                     /gene="glpT"
                     /locus_tag="OO568_001779"
     CDS             complement(6661..8019)
                     /gene="glpT"
                     /locus_tag="OO568_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6641154.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8292..9920
                     /gene="glpA"
                     /locus_tag="OO568_001780"
     CDS             8292..9920
                     /gene="glpA"
                     /locus_tag="OO568_001780"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6641155.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIAHHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9910..11169
                     /gene="glpB"
                     /locus_tag="OO568_001781"
     CDS             9910..11169
                     /gene="glpB"
                     /locus_tag="OO568_001781"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6641156.1"
                     /translation="MRFDTVIMGSGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNKENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQITQRAGGQQ"
     gene            11166..12356
                     /gene="glpC"
                     /locus_tag="OO568_001782"
     CDS             11166..12356
                     /gene="glpC"
                     /locus_tag="OO568_001782"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6641157.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12549..13448
                     /locus_tag="OO568_001783"
     CDS             12549..13448
                     /locus_tag="OO568_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6641158.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDESDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLAATQLTDADIPNCY"
     gene            13452..13688
                     /locus_tag="OO568_001784"
                     /pseudo
     CDS             13452..13688
                     /locus_tag="OO568_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13729..14199)
                     /locus_tag="OO568_001785"
     CDS             complement(13729..14199)
                     /locus_tag="OO568_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6641159.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14254..15456)
                     /locus_tag="OO568_001786"
     CDS             complement(14254..15456)
                     /locus_tag="OO568_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6641160.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVSFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15543..16085)
                     /locus_tag="OO568_001787"
     CDS             complement(15543..16085)
                     /locus_tag="OO568_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641161.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16364..16789
                     /gene="nudI"
                     /locus_tag="OO568_001788"
     CDS             16364..16789
                     /gene="nudI"
                     /locus_tag="OO568_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6641162.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16828..17430)
                     /locus_tag="OO568_001789"
     CDS             complement(16828..17430)
                     /locus_tag="OO568_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6641163.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17738..18877
                     /gene="arnB"
                     /locus_tag="OO568_001790"
     CDS             17738..18877
                     /gene="arnB"
                     /locus_tag="OO568_001790"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6641164.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDTIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18881..19849
                     /gene="arnC"
                     /locus_tag="OO568_001791"
     CDS             18881..19849
                     /gene="arnC"
                     /locus_tag="OO568_001791"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6641165.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19849..21831
                     /gene="arnA"
                     /locus_tag="OO568_001792"
     CDS             19849..21831
                     /gene="arnA"
                     /locus_tag="OO568_001792"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR6641166.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYDS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21828..22718
                     /locus_tag="OO568_001793"
     CDS             21828..22718
                     /locus_tag="OO568_001793"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6641167.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22718..24363
                     /gene="arnT"
                     /locus_tag="OO568_001794"
                     /pseudo
     CDS             22718..24363
                     /gene="arnT"
                     /locus_tag="OO568_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
     gene            24360..24695
                     /gene="arnE"
                     /locus_tag="OO568_001795"
     CDS             24360..24695
                     /gene="arnE"
                     /locus_tag="OO568_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6641168.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24695..25081
                     /gene="arnF"
                     /locus_tag="OO568_001796"
     CDS             24695..25081
                     /gene="arnF"
                     /locus_tag="OO568_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6641169.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25075..25347)
                     /gene="pmrD"
                     /locus_tag="OO568_001797"
     CDS             complement(25075..25347)
                     /gene="pmrD"
                     /locus_tag="OO568_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6641170.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25452..26807)
                     /gene="menE"
                     /locus_tag="OO568_001798"
     CDS             complement(25452..26807)
                     /gene="menE"
                     /locus_tag="OO568_001798"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6641171.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26804..27766)
                     /gene="menC"
                     /locus_tag="OO568_001799"
     CDS             complement(26804..27766)
                     /gene="menC"
                     /locus_tag="OO568_001799"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6641172.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(27766..28623)
                     /gene="menB"
                     /locus_tag="OO568_001800"
     CDS             complement(27766..28623)
                     /gene="menB"
                     /locus_tag="OO568_001800"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6641173.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28638..29396)
                     /gene="menH"
                     /locus_tag="OO568_001801"
     CDS             complement(28638..29396)
                     /gene="menH"
                     /locus_tag="OO568_001801"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6641174.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGVAG
                     LCGVIIEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29393..31063)
                     /gene="menD"
                     /locus_tag="OO568_001802"
     CDS             complement(29393..31063)
                     /gene="menD"
                     /locus_tag="OO568_001802"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6641175.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIGNIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31152..32447)
                     /gene="menF"
                     /locus_tag="OO568_001803"
     CDS             complement(31152..32447)
                     /gene="menF"
                     /locus_tag="OO568_001803"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6641176.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAKGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGERLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32526..32831)
                     /gene="elaB"
                     /locus_tag="OO568_001804"
     CDS             complement(32526..32831)
                     /gene="elaB"
                     /locus_tag="OO568_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6641177.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGEPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32886..33347)
                     /locus_tag="OO568_001805"
     CDS             complement(32886..33347)
                     /locus_tag="OO568_001805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6641178.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDCIPHIGMAREVIQA"
     gene            33412..34329
                     /gene="rbn"
                     /locus_tag="OO568_001806"
     CDS             33412..34329
                     /gene="rbn"
                     /locus_tag="OO568_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6641179.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34517..35191
                     /locus_tag="OO568_001807"
                     /pseudo
     CDS             34517..35191
                     /locus_tag="OO568_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8654 a         9397 c         8690 g         8450 t
ORIGIN      
        1 atgcaatggc tattttgtta ttatttgatg aataatatca gtgcgtcata attcaagtta
       61 ataaccttaa gggataccag ttatatttaa actaaattaa agtaatgaat aattttctta
      121 taatataagg taaattaaca aaatggcttc gcatttaaca ataaccgaat agaaaacaac
      181 catttcgaca tcaacaatcg cttacattcg cttatatatt gaccacaact gatacatcag
      241 attatgtgat gactcgtgct tagatcaatt tttgcaatca ttagcaaaaa gattaataaa
      301 ccatctatat caatttatcc aacctattct gccgttcaag aaatcgccga acagttattt
      361 ttaacaaatt tttctcttcc cattgacttt cccggacacc ttgtctgacc taaggtgcgc
      421 gaaagccact ttttccttcc tgagttatcc acaaagttat gcacttgcaa gagggtcatt
      481 ttcacactat cttgcagtga atcccaaaca taccccctat atatagtgtt ctaagcagct
      541 tcccttacta caggtagtct gcatgaaact attgcggaaa gaattccaaa aacaggtacg
      601 acatacatga atcagaatct gctggtgaca aagcgcgacg gtagcacaga gcgcatcaat
      661 ctcgacaaaa tccatcgcgt tctggattgg gcggcagaag gactgcataa cgtttcgatt
      721 tcccaggtcg agctgcgctc ccacattcag ttttatgacg gtatcaagac ctctgacatc
      781 cacgaaacta ttatcaaggc tgccgcagac ctgatctccc gtgatgcgcc ggattatcag
      841 tatctcgccg cgcgcctggc gatcttccac ctgcgtaaaa aagcctacgg ccagtttgag
      901 ccgcctgcgc tgtacgacca cgtggtgaaa atggtcgaga tgggcaaata cgataatcat
      961 ctgctggaag actacacgga agaagagttc aagcagatgg acacgtttat cgatcacgac
     1021 cgtgatatga ccttctctta tgctgccgtt aagcagctgg aaggcaaata tctggtacag
     1081 aaccgcgtga ccggcgaaat ctacgaaagc gcccagttcc tttatattct tgttgccgcg
     1141 tgcctgttct cgaactaccc gcgtgaaacg cgcctgcaat acgtgaagcg tttttacgac
     1201 gcggtttcca catttaaaat ttcgctgccg acgccaatca tgtccggcgt gcgtaccccg
     1261 actcgtcagt tcagctcctg cgtactgatc gagtgcggtg acagcctgga ttccatcaac
     1321 gccacctcca gcgcgattgt gaaatatgtt tcccagcgtg ccgggatcgg catcaacgcc
     1381 ggacgtattc gtgcgctggg cagcccgatt cgcggtggcg aagcgttcca taccggctgt
     1441 atcccgttct acaaacattt ccagacagcg gtgaaatcct gctctcaggg cggtgtgcgc
     1501 ggcggtgcgg caacgctgtt ctacccgatg tggcatctgg aagtggaaag cctgctggtg
     1561 ttgaaaaaca accgtggggt tgaaggcaac cgcgtgcgtc atatggacta cggggtacaa
     1621 atcaacaaac tgatgtatac ccgcctgctg aaaggtgaag atatcaccct gttcagcccg
     1681 tccgacgtac cggggctgta cgacgcgttc ttcgccgatc aggaagagtt tgaacgcctg
     1741 tataccaaat atgagaaaga cgacagcatc cgcaagcagc gtgtgaaagc cgttgagctg
     1801 ttctcgctga tgatgcagga acgtgcgtct accggtcgta tctatattca gaacgttgac
     1861 cactgtaata cccatagtcc gtttgatccg accatcgcgc cagttcgcca gtctaacctg
     1921 tgcctggaaa ttgccctgcc gaccaaaccg ctgaacgacg tcaacgacga gaacggtgaa
     1981 atcgcgctgt gtacgctgtc tgctttcaac ctgggcgcaa ttaatagcct ggatgaactg
     2041 gaagagctgg cgattctggc ggttcgtgca cttgacgcgc tactggatta tcaggattac
     2101 ccgatcccgg ccgccaaacg tggagcgatg gggcgtcgta cgctgggtat tggtgtgatt
     2161 aacttcgctt actatctggc gaagcacggt aaacgctact ccgacggcag cgccaacaac
     2221 ctgacgcata aaaccttcga agcgattcag tattacctgc tgaaagcgtc taatgagctg
     2281 gctaaagagc aaggcgcgtg cccgtggttt aacgaaacca cttacgcgaa agggatcctg
     2341 ccgatcgata cctataagaa agatctggat gccatcgcta atgagccgct gcattacgac
     2401 tgggaagcat tgcgtgaatc aatcaaaacg cacggcctgc gtaactccac gctttctgcc
     2461 ctgatgccgt ccgagacctc ttcgcagatc tctaacgcca ctaacggtat tgaaccgccg
     2521 cgcggttacg tcagcatcaa agcgtcgaaa gacggtattt tgcgccaggt ggtgccggac
     2581 tacgagcacc tgcacgacgc ctatgagctg ctgtgggaaa tgccgggtaa cgatggctac
     2641 ctgcaactgg taggtatcat gcagaaattt atcgatcagt cgatctctgc taacaccaac
     2701 tacgatccgt cacgtttccc gtcaggaaaa gtgccgatgc agcagttgct gaaagacctg
     2761 ctcaccgcct acaaattcgg ggtcaaaacg ctgtattatc agaacacccg tgacggcgct
     2821 gaagacgcgc aggacgatct ggtaccgtca atccaggacg atggctgcga aagcggcgca
     2881 tgtaagatct gatattgaga tgccggatgc ggcgtaaacg ccttatccgg cctacggctc
     2941 ggtttgtagg cctgataaga cgcgccagcg tcgcatcagg ctccgggtgc cggatgcagc
     3001 gtgaacgcct tatccggcat taaactccca acaggacaca ctcatggcat ataccacctt
     3061 ttcacagacg aaaaatgatc agctcaaaga accgatgttc tttggtcagc cggtcaacgt
     3121 ggctcgctac gatcagcaaa aatatgacat cttcgaaaag ctgatcgaaa agcagctctc
     3181 tttcttctgg cgtccggaag aagttgacgt ctcccgcgac cgtatagatt accaggcgct
     3241 gccggagcac gaaaaacaca tctttatcag caacctgaaa tatcagacgc tgctggattc
     3301 cattcagggt cgtagcccga acgtggcgct attgccgctt atttctattc cggaactgga
     3361 aacctgggtc gaaacctggg cgttctcaga aacgattcat tcccgttcct atactcatat
     3421 cattcgtaat atcgttaacg atccgtctgt tgtgtttgac gatatcgtca ccaacgagca
     3481 gatccagaaa cgtgcggaag ggatctccag ctattacgat gaactgatcg aaatgaccag
     3541 ctactggcac cttctgggcg aaggcaccca caccgttaac ggtaaaaccg taaccgttag
     3601 cctgcgcgag ctgaagaaaa aactgtatct ctgcctgatg agcgttaacg cgctggaagc
     3661 gatccgcttc tacgtcagct ttgcttgttc ttttgccttt gcagaacgcg aactgatgga
     3721 aggtaacgcc aagattattc gcctgattgc ccgcgatgaa gccctgcacc tgaccggtac
     3781 tcagcatatg ctgaatctgc tgcgcagcgg cgcggatgat ccggagatgg cggaaattgc
     3841 cgaagaatgt aagcaggagt gctatgacct gtttgtccag gcggcacagc aagagaaaga
     3901 ctgggcggat tatctgttcc gtgacggttc gatgattggt ctgaataaag atattctctg
     3961 ccagtacgtt gaatacatca ccaatatccg catgcaggca gtcggtttgg atctgccgtt
     4021 ccagacgcgc tccaacccga tcccgtggat caacacctgg ctggtgtctg ataacgtgca
     4081 ggttgctccg caggaagtgg aagtcagttc ttatctggtc gggcagattg acgcggaagt
     4141 ggacaccgac gatttgagta acttccagct ctgatggccc gcgttaccct gcgcatcact
     4201 ggcacacaac tgctgtgcca ggatgaacac ccttcccttc tggcggcgct ggaatcccac
     4261 aatgtggcgg ttgagtacca gtgccgcgaa ggttactgcg gctcctgtcg cacccgactg
     4321 gtcgcaggtc aggtggactg gattgccgaa ccgttagcct ttattcagcc gggggaaatt
     4381 ttgccctgtt gttgccgggc aaaaggcgat attgaaatcg agttgtgatc gggtactagc
     4441 gccagatacg acgcttacgc gtattatctg gcctacaacg attacatggc gtagtaatac
     4501 gctttcacct gctcccagtc cgctttaggg atcggctcca gatatttttc cagctggcgg
     4561 aagtcatgat taatcgcttt atcgcgacgc aagcggcgac gacttttctc cagatcaaga
     4621 aaaccagctt ctgccttacc ttctgttttc acatagatat ggcgaacata gcaacagcca
     4681 tgctgacgat taacgctatg cattttctta aacgccagtg ccactgcttt caacatggct
     4741 tgccgtactt cgtcagaata aggcgatact gcatgccggg cataccagtc agcaatgctg
     4801 atgaaccccg ccatatcttc agtcaccagc aacgcgcgcc attcaccctc aattttcacc
     4861 gcttcaccaa aaacgatctt cggcacgatg acaccggccc gttccagttc tttaattacc
     4921 gcaacctcac ggacaatcgt tggtcggccg aacggataac gtactgaatg aaacagatga
     4981 tgcgtcatgc gctttacata cagctttttg ccgttgcgct cgacgcattg caccctgctc
     5041 ataccattac ggcgatagtt aggctcttca acccagtcgc cctctgttgc ccaccagtga
     5101 ttaaattcgt cgtactttgc tgaaactgcc ataccctatc gcctgtcatt tttattaacg
     5161 acaatgacta taggtggtta cctgaggaaa atcttaatga aacgtgtcgt attccgctac
     5221 aggtgtgaca ccgcttttgc ccttcatttg cagcgcatag attccaccag ccactaccac
     5281 taatagtgcg cccagcagat aaaatgtgaa tttaatcagt cggatacagc gatgaatttt
     5341 attcgacagg gattaggcat cgccttacaa ccagagttaa cgctgaaaag cattgcaggt
     5401 gaattgtgtt ccgttcctca cgaaccaacc ttctatcgac agatttcgtt gctgactaaa
     5461 gaaaagccgg tagaaggcag tccactgttt ttactacaaa tgtgcatgga acaattagtg
     5521 gcgattggaa aaatttgata tgagacagga tggcgcatga acgccatcct gcagtaacat
     5581 tactctttat taagaaattt tactgcctta tcagggaaat cagtaaacag cccatttaca
     5641 cccgctttgt tatacagagc atcatataac tgattcacat caggagtgta ttcaggcagt
     5701 ttatctgacc gcacggtata aggatgcact accagtttat tctgctgagc atcttgcacc
     5761 atgccagtga gtttgatatt acccggctgc gatgtctcct caatcaacat atggtaatcc
     5821 ggaccaatac catctgcata ttccgccacc tgtttcatgg cacccggctt aaacatccag
     5881 tcgtagttgt aattaaccca gcttccatcc ggctgtttct gctgcgtttc attccagtcg
     5941 gtataggcaa tcagctgtac cagattgagc tccatgccca ttttgggttc cagctcattc
     6001 ttaatacgct tcagctcatc agcatcaaaa cattgcaaat aaactttatc gtctttaccg
     6061 gtgtaaccat atttcttcag cacttccagc gtttttgccg caatatcctt cccttcctga
     6121 tgatggaacc acggcgcttt gatttctgga tagataccga tatttttccc ggtagagtga
     6181 tttaacccct gaacaaattc aatctcttct tcaaaggtgt gcacccggaa gtcggactta
     6241 cccattggga aacgccccgg ataagtctgt acttttttac cgttttcaat atcgaaacct
     6301 tcggtaaatt tcagcgactt aatttcatcc agcgtgaaat ctatcgcgta gtaacgaccg
     6361 tctttgcgcg cccgatccgg gaaacgatcg gcaacatcag taacacgatc gagataatgg
     6421 tcatgcagaa caaccagatg gtcgtctttg gtcatcacca aatcctgttc cagataatcc
     6481 gctccctgcg catacgccat cgcttttgct ggcagcgtat gctccggcaa atatccactg
     6541 gcaccgcgat gggcgatgac tattttttcg ttgctgtccg ccgccattgc actgcttccc
     6601 atgactatag tgctcatcat gatcgccatg ctaaggtttt tcagcttcaa tttcatgcca
     6661 ttagcctccg ttgcgttttt gcagtaattg ttcatggcga cgtttttcgc caatcatcac
     6721 aacaatcaac aagataaccg ccagaatgct gccgccaatc attaccataa agccgccatc
     6781 ccagccgaag aagtccacgg tgtagccaac aatcgcgctc gccgccaccg aaccgcccag
     6841 gtaaccaaac agcccggtaa agcccgctgc cgtacctgcc gcttttttcg gtgccagttc
     6901 cagcgcatgc agaccgatca gcatcacagg accgtagatc aggaagccga taacaatcat
     6961 acaaatcata tcgacggttg ggttacctgc cgggttcatc cagtaaacga tagtcgcgat
     7021 ggtcaccagt gtcataaaga agacgccggt tgccccacgg ttaccacgga agactttgtc
     7081 cgacatccag ccgcacagca gggtgcccgg aatacccgca tactcataga agaagtaggc
     7141 ccaggaggat ttatccagcg cgaaatgctt aacctctttc agataagtcg gtgaccagtc
     7201 gaggatgccg taacgcagca gataaacgaa cacgttggcg atggcgatgt accacagcag
     7261 tttgttcggc agcacgtact gcatgaagat ttgcttcgcg gtcagctcct gttccgcttt
     7321 ttcgttatag tcgtccggat aatcattttt gtactcttcg atcggcggca agccacagga
     7381 ttgcggggta tcgcgcatca tcgcaaaggc gaataatgcc accagaatgg cgcagaaagc
     7441 aggcatatag agcgccgcat gccagtcatt gaaccaggcc atccccagca ggaacagcag
     7501 cggcggaata ccaccaccga cgttgtgcgc acagttccac actgacacaa tgccgccacg
     7561 ttctttctgc gaccaccagt gcaccatagt acgaccacac ggcggccacc ccatcccctg
     7621 gaaccaaccg cagaggaaca acagtacaaa catcaccgca atgctcgacg tcgcccatgg
     7681 cacaaagccc ataaacaaca tcactgccgc cgccagaatc aaacctgcgg gcaggaaaac
     7741 gcgcggattc gagcgatccg ataccgaacc catgatgaat ttcgaaaatc cataagcaat
     7801 cgagatcccc gaaagggcaa aacctaaatc accgcgtgag aatccctgct caaccagata
     7861 aggcatagca agcgcaaagt tcttacgaac caaatagtaa gccgcatagc caaagaatat
     7921 ccccaggaaa atttgccagc gcaatcgacg ataagtcgga tcgatctccg cggcaggtaa
     7981 gcgcgctttg tgtggcgctg gtttaaaaat actcaacatt gatagcctcc gtggcccgtg
     8041 gtcttattta tgattaacag cctgattcag tgagagaacc tgccgtttct tgagttgccg
     8101 cgatgttaag aaaacattca taaattaaat gtgaattgcc gcacacatta ttaaataaga
     8161 tttacaaaat gttcaaaatg acgcatgaaa tcacgtttca ctttcgaatt atgagcgaat
     8221 atgcgcgaaa tcaaacaatt catgttttta ctatggctaa atggtaaaaa acgaacttca
     8281 gagggataac aatgaaaact cgcgactcgc aatcaagtga cgtgattatc attggcggcg
     8341 gcgcaacggg agccgggatt gcccgcgact gtgccctgcg cgggctgcgc gtgattttgg
     8401 ttgagcgcca cgacatcgca accggtgcca ccgggcgtaa ccacggcctg ctgcacagcg
     8461 gtgcgcgcta tgcagtaacc gatgcggaat cggcccgcga atgcattagt gaaaaccaga
     8521 tcctgaaacg cattgcacat cactgcgttg aaccaaccaa cggcctgttt atcaccctgc
     8581 cggaagatga cctctcattc caggccactt ttattcgcgc ctgcgaagaa gcagggatca
     8641 gcgcagaagc tatagacccg cagcaagcgc gcattatcga acctgccgtt aacccggcac
     8701 tgattggcgc ggtgaaagtt ccggatggca ccgttgatcc atttcgtctg accgcagcaa
     8761 acatgctgga tgccaaagaa cacggtgccg ttatccttac cgctcatgaa gtcacggggc
     8821 tgattcgtga aggcgcgacg gtgtgcggtg ttcgtgtacg taaccatctc accggcgaaa
     8881 ctcaggccct tcatgcacct gtcgtggtta atgccgctgg gatctggggg caacacattg
     8941 ccgaatatgc cgatctgcgc attcgcatgt tcccggcgaa aggatcgctg ctgatcatgg
     9001 atcaccgcat taaccagcat gtgatcaacc gctgccgtaa accttccgac gccgatattc
     9061 tggtgcctgg cgataccatt tcgctgattg gtaccacctc tttacgtatt gattacaacg
     9121 agattgacga taatcgagtg acggcagaag aggttgatat tctgctgcgt gaaggggaaa
     9181 aactggcccc cgtgatggcg aaaacgcgca ttttgcgggc ctattctggc gtgcgcccgc
     9241 tggttgccag cgatgacgac ccgagcggac gtaacgtcag ccgtggcatc gtgctgctcg
     9301 accatgctga acgcgatggt ctggacggat ttatcaccat caccggtggc aaactgatga
     9361 cctatcggct gatggctgaa tgggctaccg acgcggtatg ccgcaaactg ggcaacacgc
     9421 gcccctgtac gactgccgat ctggcactgc ctggttcaca agaacccgct gaagttacct
     9481 tgcgtaaagt catctccctg cctgccccgc tgcgcggttc tgcggtttat cgtcatggcg
     9541 atcgcacgcc tgcctggctg agcgaaggcc gtctacaccg tagcctggta tgtgagtgcg
     9601 aagcggtaac tgcgggtgaa gtgcagtacg cggtagaaaa tttaaacgtt aatagcctgc
     9661 tggatttacg ccgtcgtacc cgtgtgggga tgggcacctg ccagggcgaa ctctgcgcct
     9721 gccgcgctgc cggactgctg caacggttta acgtcacgac gtccgcgcaa tctatcgagc
     9781 aactttccac cttccttaac gaacgctgga aaggcgtgca acccatcgcc tggggagacg
     9841 cactgcgcga aagcgaattt acccgctggg tttatcaggg actgtgtggt ctggagaagg
     9901 agcagaaaga tgcgctttga tactgtcatt atgggcagcg gcctcgccgg attactctgt
     9961 ggcctgcaac tgcaaaaaca cggcctgcgc tgtgccattg tcacgcgtgg tcaaagcgcg
    10021 ttgcatttct catccggttc gctggatttg ctgagccatc tgccagatgg tcaacccgtg
    10081 gcagacattc acagtggact ggaatctttg cgtcagcagg cacctgcaca tccttactct
    10141 cttctcgggc cacaacgcgt gctcgatctc gcttgccagg cgcaggcatt aatcgctgaa
    10201 agcggtgcgc aattgcaggg cagcgtagaa ctggctcacc agcggattac gccgctcggc
    10261 actttgcgtg caacctggtt aagttcgcca gaagttcccg tctggccgct acccgcgaag
    10321 aagatatgtg tagtggggat aagcgggctg atggatttcc aggcgcatct tgcggcggct
    10381 tcgttgcgcg aactggatct ttcggttgaa actgcagaaa tagagctgcc ggaactggat
    10441 gtcctgcgca ataacgccac cgaatttcgc gcggtgaata tcgcccgttt ccttgataat
    10501 aaagaaaact ggccgctgtt acttgatgcg cttattcctg tcgccaatac ctgcgaaatg
    10561 atcctgatgc ccgcctgctt cggcctagcc gatgacaaac tgtggcgttg gttgaatgaa
    10621 aaactacctt gttcactgat gcttttgcca acgctgccgc cttccgtgct gggcattcgt
    10681 ctgcaaaacc agttacagcg ccagtttgtg cgccagggtg gagtgtggat gccgggcgat
    10741 gaagtgaaaa aagtgacctg taaaaatggc gtagtgaatg aaatctggac ccgcaatcac
    10801 gcagatattc cgctacgtcc acgtttcgcg gttctcgcca gcggcagttt ctttagtggc
    10861 ggactggtag cggaacgtaa cggcattcgc gagccgattc tcggccttga tgtgctacaa
    10921 accgccacgc ggggtgaatg gtataagggc gatttttttg cgccgcaacc gtggcagcag
    10981 ttcggtgtaa ccactgatga gacgctacgc ccgtcgcagg cagggcaaac cattgagaac
    11041 ctgtttgcca tcggttcggt gctgggcgga tttgatccca tcgcccaggg atgcggcggc
    11101 ggtgtttgtg ccgtcagtgc tttacatgcc gctcaacaga ttacccaacg cgcaggaggc
    11161 caacaatgaa cgacaccagc ttcgaaaact gcattaagtg caccgtctgc accaccgcct
    11221 gcccggtgag ccgggtgaat cccggttatc cagggccaaa acaagccggg ccggatggcg
    11281 agcgtctgcg tttgaaagat ggcgcactgt atgacgaggc gctgaaatat tgcatcaact
    11341 gcaaacgttg tgaagtcgcc tgcccgtccg atgtgaagat tggcgatatt atccagcgcg
    11401 cgcgggcgaa atatgacacc acgcgcccgt cgctgcgtaa ttttgtgttg agtcataccg
    11461 acctgatggg tagcgtttcc acgccgttcg caccaatcgt caacaccgct acctcgctga
    11521 aaccggtgcg gcagctgctt gatgcggcgt taaaaatcga tcatcgtcgc acgctaccga
    11581 aatactcctt cggcacgttc cgtcgctggt atcgcagcat agcggctcag caagcacaat
    11641 ataaagacca ggtcgctttc tttcacggct gcttcgttaa ctacaaccat ccgcagttag
    11701 gtaaagattt aattaaagtg ctcaacgcaa tgggtaccgg tgtacaactg ctcagcaaag
    11761 aaaaatgctg cggcgtaccg ctaatcgcca acggctttac cgataaagca cgcaaacagg
    11821 caattacgaa tgtagagtcg atccgcgaag ctgtgggagt aaaaggcatt ccggtgattg
    11881 ccacctcctc aacctgtaca tttgccctgc gcgacgaata cccggaagtg ctgaatgtcg
    11941 acaacaaagg cttgcgcgat catatcgaac tggcaacccg ctggctgtgg cgcaagctgg
    12001 acgaaggcaa aacgttaccg ctgaaaccgc tgccgctgaa agtggtttat cacactccgt
    12061 gccatatgga aaaaatgggc tggacgctct acaccctgga gctgttgcgt aacatcccgg
    12121 ggcttgagtt aacggtgctg gattcccagt gctgcggtat tgcgggtact tacggtttta
    12181 aaaaagagaa ctaccccacc tcacaagcca tcggcgcacc actgttccgc cagatagaag
    12241 aaagcggcgc agatctggtg gtcaccgact gcgaaacctg taaatggcag attgagatgt
    12301 ccacaagtct tcgctgcgaa catccgatta cgctactggc ccaggcgctg gcttaaactc
    12361 ccttctgatg cccggtaagc atgtggttac cgggcatttt tgcgtacacg attccgtgcc
    12421 caatgtatgc gttgcaacgc agtgaaaatt cctctgaaaa cgtctcgcaa aggctgaaac
    12481 tggcagatgt caaaggcctg agataaccgt aatgtcgcgt catcataaat atcaggtgac
    12541 ggacaaccat gaccgaatca acaacctcct ccccgcatga tgcggtattt aaaaccttta
    12601 tattcacacc cgaaaccgca cgggattttc tcgaaataca tttaccagaa ccactgcgca
    12661 agctttgcaa cctgcaaacc ttacgcctgg aacccactag ttttattgaa aaaagtttac
    12721 gcgcttacta ctcggatgtt ttgtggtccg tggaaaccag cgacggtgac ggctatatct
    12781 actgcgtgat tgaacatcaa agctctgcag aaaagaatat ggcttttcgg ccaatgcgct
    12841 atgccactgc cgccatgcag cgtcacctag ataaaggcta tgacagagtt ccgctggtgg
    12901 tgccattgct gttttatcat ggcgaaacat cgccctaccc gtactcactt aactggctgg
    12961 atgagtctga cgatccgcaa cttgcccggc agttgtacac cgaagctttt ccgttggtgg
    13021 atatcaccat cgtacctgac gatgagatca tgcaacatcg gcgtatagct ctgctggaac
    13081 tgattcaaaa gcatattcgc gaccacgatt taatcggcat ggtcgacagg atcaccacgc
    13141 ttttggttag aggcttcact aatgacagcc agctacaaac actgtttaat tatctgctgc
    13201 aatgcggcga tacctcccgt ttcacccgtt ttattcagga gattgccgaa cgttcaccac
    13261 tacaaaagga gagattaatg actattgctg aacggctacg gcaggaaggg catcaaattg
    13321 gctggcagga aggtatgcat gaacaagcca ttaaaattgc cttgcgcatg ctggagcagg
    13381 gctttgaacg tgagattgtg ctggcggcaa cccaactcac tgatgctgat attccgaact
    13441 gttattaaaa ggagagggag agattgatga ctattgctga acggctacgg caggaaggac
    13501 atcaaattgg ctggcaagaa ggtaaattag taggtttgca gcaaggtaaa ttagaaggtt
    13561 tgcagcaagg tatgcatgaa caagccatta aaattgcttt gcgcatgctg gaacagggca
    13621 ttgatcgtga ccaagtgctc gcggccaccc agctaagcga agccgatctg gcagcgaata
    13681 accactaatt aacacaggcc cacagccgat ccccatgggc ctttgatatc aataactacc
    13741 ttttatgcgt ggcccatttt tgcctgattt aaacatcgcc agtcgttgat ccagggcatc
    13801 gctgtagagc atcgtgtcaa cgccaacagc gacaaagttc gctccccacg ccaggcattg
    13861 ctgcgccata tcaggagcca cagccagaaa accagccgct ttacccgcag cacggatccg
    13921 ccgaatgctg gtttcaataa ttcgctgcac ttccgggtgc ccggcgttat ccgggtagcc
    13981 caacgacgca gaaagatccg ctggtccaat aaacacgcca tcaatccctt cgacgtcgag
    14041 gatctcgtcc aggttatcca gtgccgtttt actttccacc tgcactaaca gacaaagcga
    14101 atcgttgact tgcgccatgt aattctcaat gcgtccccag cgcgcagccc gtgccacact
    14161 ggccccaaca ccacgctcac cgtagggagg gttggacaaa ttttgccgga gggggaatgt
    14221 gccggtgcag taaaccggca cagagtggcg aaatcagacg gataaagtca tggactcaac
    14281 aacctcaatc cagccatgct cactagcgat gtcctggccg tttaaccaac ggcgtagcat
    14341 attcagtgcc atcattgcgc acacttcctg acggatagcc aggctgtagc gtgtagtgct
    14401 gaaactcaca cgcagagcga aagtgccgtc tggcgtggct agcgcaaagt tgagatgctc
    14461 gttctcgaaa cccgaaacag ccagtgccag cccggcaaaa tggttggccc gccgttctgt
    14521 aatccagtgc gcagtttgcg ccagggtttc ttcctgtgaa ggaatcactt cacacgccag
    14581 caatggagca cctgcgcgag aaagttgcaa agccaataaa ccaccggtga attgctcgct
    14641 caacgtcagg ctgaactggc ggttttgcaa ttcgcgactg atctgcgcgg gcagtccttc
    14701 ggtgccttcg aaaatcacgc tctgtccggc aacacgttta acgtccagcc acagtttttc
    14761 catcgcctgt tgctcgcttg ccggtccggt gagtttcagt tcgatgatcg gcattgagga
    14821 gcgatagccc attgttacgc ccggcggcag ttgtagagtg tccaggcttt gtgccagatc
    14881 gctttccgaa cgaccaaagg tagttaaacg cagacaaacc ggcggctgtg gtaaagaaaa
    14941 acgctcgcgc aggcgcggca ggatttcgtg ctcgaccatc accttaaatt ctgacggtac
    15001 gccgggagta aagaacatca ggcaacgatt aagctgcacg gcaaaaccac aggcggtgcc
    15061 taccgggtta ttgataaatt cagcactggc aggcagctcc gcttgtttac ggttgctcgg
    15121 tgccattact cgtccacgtt cgtggaaata gcgttccatc tctttgagcc aggcttcatg
    15181 cagcacaagg ccttcacctt ttgctgtcgc agcggcgagt gcgcttaaat catcgctggt
    15241 cggtcccagc ccgccgttaa cgatcagcac atcggcgtgc tgactacgtt cgcgaagaat
    15301 ggtgactaag tcatcaaggt tatcccccac cgtattgcgg cgagataatg gcagcccctg
    15361 atgaaagaaa aaatcggcca gccaggcagc gttagtgtca acgatttgcc cgtataacac
    15421 ttcatccccg gtggatagca tttccacttt taacatggtt atctcctgct tttgttatcg
    15481 aatcactata acgcaaaggg agataaggcg tagtaagaaa ctgaatgccg cgccggaagg
    15541 gatcagaaac tggcgttaac cccaacatac aggccgtcag ccacagcgtt gtcgcggtta
    15601 ccgtctttac ccgacagatt caggtagcga taacccgcct caatactgac tggacgcata
    15661 atggtgtaac gcgcgccagc attcgcttcc tcataacttt gaataccgct ggagagcgaa
    15721 tccggagagt agtaatactc gccaaacaaa cggaagctgt tgccaatttt ccactgcaaa
    15781 ccacctccta ccgctgcggc ataaccttca tcgccgtaat tcgggttggt gtacacgcct
    15841 tttccgccaa cggtcgccat taacggcccg agaggcagat tcaaccccag ccccacgccc
    15901 gcgacgtcac cgtcgtcgtc gttatgtgtc cagttaccgc ttaaagccag gcccgtcgat
    15961 tcagtcccaa aaccgacacc aatattggtg tattctttac ccgcctggcc gctgatgctc
    16021 attgcattga ccgatgcaga aaccaacagc attccggcca gacctgctag cgcaattttt
    16081 ttcattatcc tctttcccac aagcaattaa tcaaaattcc caaagctgcg ggattgtact
    16141 gcctggcaca gaggattcaa tgagcgaaaa aagggatcta gtcacttatt gtaactaaat
    16201 acttcttaaa taattaaacg ccgaaaaaat aaacacactt taccgataca cacaaaaata
    16261 ataataagtg cagttatcat tgcaaaagag attactgtca ctttcacata tttctgagtt
    16321 accgtattct catggtttct ttttatttat aaggaatacc cctgtgcgac aacggactat
    16381 tgtatgccct ttgattcaaa atgatggtgc ttatttgctg tgcaaaatgg ccgacgatcg
    16441 cggcgttttc cccggtcaat gggcgctttc gggtggcggc gtggagtctg gcgaacgaat
    16501 tgaagaggca ctacgccgcg aaattcgcga agaactggga gaacagctgc ttttgacaga
    16561 aatcacgccg tggaccttca gcgatgatat tcgcaccaag acatatgcag atggtcgcaa
    16621 ggaagagatt tatatgattt acctgatttt tgactgcgtt tctgccaacc gagaagtgaa
    16681 aataaacgaa gagtttcagg actacgcgtg ggtaaaacct gaagatctgg tgcattatga
    16741 tttaaatgtt gccacccgaa aaacgttacg tttgaaaggt cttctttaac aatgatgaca
    16801 gccatcactg gctgtcagat tgaaaattta gtggttaacg aattccccat ccagataaac
    16861 tttgcctttt tccacatgca tgactaaacc atccagataa tcaggtttaa atatcgcatc
    16921 acgcttattt ttagcaatat atgtcaggca atgattatgg gtgaaaataa cgatattttt
    16981 atcaggcgct ttgctttgta agtccttaat tgcactataa atctcattac cgcactgaag
    17041 aagtcgttta tctaccgtca gctttttacc cgcggaaaac caggtcgccg actgaatggt
    17101 ccggacggta ttactggaat aaagatcgaa atcagggatg tcagcgctaa aagcgttgcc
    17161 cagttcacgg gcatcctggg tacctttaac cgtaatacct gttttatctg acaagcattg
    17221 attggttgaa cgatcgcaac gttcagcatg gcgaaacaaa acgacaaccg ggtgctgctg
    17281 tgccagtctg gccaatgttt tattgtcgat acgtggcaaa ccattgctac tccaggcggc
    17341 atgagtaccc agtccggcaa ttgcagcgag cgccagtaaa atgatgaaat atttttttga
    17401 cttcaatgat gagcggcaaa aagctaacac gtaaactcca cctgtagaca agcgcaacca
    17461 gacaattacc gtgaaattga gcgacatttc tggcgataat tagcagttgg tgtaatatta
    17521 aaaatcctac gatgtcggca aaatgcctca aatttttgcc aaatgcaaag cctaaataag
    17581 aaaaaatata aaaatttcaa tatttacgtc taatattagt ttcttaaggt taagttaata
    17641 ttctatcctt aaaatttcgc tccaaatggc aaaatataca caacactctt tatagcaaat
    17701 ataagtggac aggtattcaa tggcggaagg aaaagcaatg tcagaatttt tgcctttttc
    17761 gcgaccagca atgggcgtgg aggaactcgc tgcagttaaa gaggttctcg aatccggttg
    17821 gatcacaacc ggtccgaaga atcaggcgct tgagcaagcc ttttgccagt tgacgggaaa
    17881 tcagcatgcc atcgcggtca gttcagccac cgccggaatg catatcacgc taatggcgtt
    17941 ggaaattggc aagggcgatg aagtgattac gccttccctg acctgggttt caaccctcaa
    18001 tatgatttcc ttgctgggtg caacgccggt aatggtggat gtcgaccgcg atacgctgat
    18061 ggtcacgcct gaagcgatcg aatcagccat tacgccacgc actaaagcca tcattccggt
    18121 gcattatgcc ggtgcgccag cagatattga caccattcgc gccattggcg aacgttacgg
    18181 catcgcagtt atcgaagatg ctgcccatgc cgtcggtacg tattacaaag ggcgacatat
    18241 tggcgcaaaa ggtaccgcta ttttttcatt tcatgccatt aaaaatatta cctgtgctga
    18301 aggtggcctg attgtaactg ataatgaaaa ccttgcccgc cagctacgga tgctgaaatt
    18361 tcacggtctg ggtgtcgatg cctatgacag acaaacctgg ggccgtgcac cgcaggctga
    18421 agtcttaaca ccgggctata agtacaatct gaccgatatt aacgccgcga ttgccctgac
    18481 acagttagtc aaattagagc acctcaacac ccgtcggcgc gaaattgccc agcaatatca
    18541 gcaagcactg gcagctctcc cctttcagcc attaagcctt cccgcctggc cgcacgttca
    18601 cgcctggcat ctgtttatta ttcgtgtcga tgaacaacgt tgtggtatca gtcgcgatgc
    18661 gttgatggaa gcgttaaaag aaagaggcat tggtaccggg ttacatttcc gcgccgctca
    18721 cacacaaaaa tattatcgcg agcgttttcc cacgctgtcg ttaccgaata ccgaatggaa
    18781 tagcgaacgc atctgttcgt tgccgctgtt cccggatatg actaccgccg atgccgaccg
    18841 cgtcatcaca gcccttcagc aactcgcagg acaataagcc atgtttgaaa tccaccctgt
    18901 taagaaagtc tcggtggtta ttcccgttta taacgagcag gaaagcttac cggaattaat
    18961 caggcgcacc accacagcct gtgaatcgtt ggggaaagag tatgagatcc tgctgattga
    19021 tgacggcagt agcgataatt ccgcacatat gctggtcgaa gcctcacaag cggagaacag
    19081 ccatattgtg tctattttgc ttaaccgcaa ttacgggcaa cattcagcga ttatggcggg
    19141 attcagtcac gttactggcg acttaattat tacccttgat gccgatctcc agaatccgcc
    19201 agaagaaatc ccccgcctgg tggcaaaagc cgatgaaggt tacgacgtgg tagggactgt
    19261 acgccagaac cgccaggaca gctggtttcg taaaacagct tcgaagatga ttaaccggct
    19321 tattcagcgc accactggca aagcgatggg tgactacggt tgtatgctgc gcgcctatcg
    19381 ccgtcatatt gtcgatgcga tgttgcactg ccatgaacgc agcaccttta tcccgattct
    19441 ggcgaatatc ttcgcccgcc gtgcaattga aattccagta catcatgccg agcgtgagtt
    19501 tggtgaatcc aaatacagtt ttatgcgcct gattaatttg atgtacgacc tggtgacctg
    19561 ccttaccaca acgccgctac gtatgctgag tctgctcggc agcattattg cgattggagg
    19621 ttttagcatt gcggtgctgc tggtgatttt acgcctgacc ttcggaccac aatgggcggc
    19681 agaaggcgtc tttatgctat ttgccgtgct gtttactttt attggcgctc agtttatcgg
    19741 catgggatta ctcggtgaat atatcggcag gatctacacc gatgtccgcg cccgcccccg
    19801 ctattttgtt cagcaagtta tccgtccatc cagcaaggaa aatgaataat gaaaaccgtc
    19861 gtttttgcct accacgatat gggatgcctc ggtattgaag ctctgctggc tgccggttac
    19921 gaaattagcg ccatttttac ccataccgat aatcccggtg aaaaagcctt ttatgattcg
    19981 gtggctcatc tggcggcgga aagagacatt ccggtttatg cgccggataa cgttaatcat
    20041 ccgctgtggg tggaacgcat tgcccaactg tcaccagagg tgattttctc tttttattat
    20101 cgccatctta tttgcgacga gatttttcag ctcgctcccg caggtgcatt taatctgcac
    20161 ggttcgctgt taccaaaata tcgtggtcgc gcgccgctga actgggtgct ggtgaacggt
    20221 gaaacggaaa cgggcgttac attgcaccga atggtaaaac gtgccgatgc tggggccatt
    20281 gtggcgcaac tgcgcgttgc cattgcgcca gacgatatcg ctattacgct gcatcataaa
    20341 ttgtgccatg ccgcgcgcca gctactggaa cagacattac ccgccattaa acacggtaat
    20401 attctggaaa tcgcccagcg cgaaaacgaa gccacctgtt ttggtcgcag aacgccggat
    20461 gacagtttcc ttgaatggca taaaccggca tccgtactgc acaacatggt acgtgccgtt
    20521 gccgatccgt ggccgggtgc cttcagctat gttggcaatc agaaattcac cgtctggtcg
    20581 tcgcgtgttc atcctcatgc cagcaaagca cagccgggga gcgtgatttc tgttgcgcca
    20641 ctgctgattg cctgtggcga tggcgcgctg gaaatcgtca ccggacaggc gggcgacggc
    20701 attactatgc agggctcgca attagcgcag atgctgggcc tggtgcaagg ttcacgcttg
    20761 aatagccagc ctgcctgcac cgcccgacgc cgtacccggg tactcatcct cggggtgaat
    20821 ggctttattg gcaaccatct gacagaacgc ctgctgcgcg aagatcatta tgaagtttac
    20881 ggtctggata ttggcagcga tgcgataagc cgttttctga atcatccaca ttttcacttt
    20941 gtcgaaggcg atatcagtat tcattccgaa tggattgagt atcacgtcaa aaaatgtgat
    21001 gtcgtcttgc cgctggtggc gatagccacg ccgattgaat atacccgcaa cccgctgcgc
    21061 gtatttgaac tcgattttga agagaatctg cgcattatcc gctactgcgt taagtaccgt
    21121 aagcgaatca tcttcccgtc aacttcagaa gtttatggga tgtgtagcga taaatacttc
    21181 gatgaggacc attctaattt aatcgtcggc ccggtgaata aaccacgctg gatttattcg
    21241 gtgtcaaaac aattacttga tcgggtgatc tgggcctatg gcgaaaaaga gggtttacag
    21301 ttcaccctct tccgcccgtt taactggatg ggaccacgac tggataacct taatgcagcg
    21361 cgaattggca gctcccgcgc tattacgcaa ctcattctca atctggtaga aggttcacca
    21421 attaagctaa ttgatggcgg aaaacaaaaa cgctgcttta ctgatatccg ggacggtatc
    21481 gaggcgttgt accgcattat cgaaaatgcg ggaaatcgct gcgacggcga aattatcaac
    21541 attggcaatc ctgagaacga agcgagcatt gaggaactgg gcgagatgct gctggcgagc
    21601 ttcgaaaaac atccgctgcg ccatcatttc ccaccgtttg cgggctttcg cgttgtcgaa
    21661 agtagcagct actacggcaa aggatatcag gacgtagagc atcgtaaacc gagcatccgc
    21721 aatgcccacc gctgcctgga ctgggagccg aaaattgata tgcaggaaac catcgacgaa
    21781 acgctggatt tcttcctgcg caccgttgat cttacggata aaccatcatg accaaagtag
    21841 gcttacgcat tgatgtcgat acctttcgtg gcacccgtga aggcgtgccg cgtctgctgg
    21901 aaatcttgag taagcataat attcaggcca gcattttttt cagcgtcggc ccggacaata
    21961 tgggccgcca tctctggcga ctggtgaagc cacagttttt gtggaagatg ctgcgctcaa
    22021 acgcggcatc gctttatggc tgggatattt tactggcagg tacggcctgg ccaggtaaag
    22081 agattggtca tgccaatgcc gatatcattc gtgaagcggc taaacatcac gaagtcggtc
    22141 tgcacgcctg ggatcaccat gcctggcaag cccgtagcgg taactgggat cggcaaacaa
    22201 tgatcgacga tattgcacgc ggtcttcgca ctctggaaga gattatcggt caaccggtaa
    22261 cctgttctgc cgctgcgggc tggcgtgccg accagaaggt gatcgaagca aaagaagcgt
    22321 tccatttgcg ctacaacagc gattgtcgtg gggccatgcc gttccgtcca ctgctcgaat
    22381 caggaaaccc tggcactgcg caaattccgg tgaccttacc cacctgggat gaagtgattg
    22441 gtcgggatgt gaaagcagaa gattttaacg gttggttact caaccgcatc ctgcgagata
    22501 aaggcacgcc ggtttatacc attcatgcag aagttgaagg ctgcgcttat cagcataatt
    22561 ttgtggatct cctcaaacgc gcagctcagg aaggcgtgac attttgccct ttaagcgaac
    22621 tgttatcaga gacgttgccg ctcggacaag ttgttcgcgg aaatattgcc ggacgtgaag
    22681 gctggctggg ttgccaacaa attgcgggta gtcgctgatg aaatcggtac gttaccttat
    22741 cggcatcttc gcgtttattg cctgctatta cctgttaccg atcagcacgc gtctgctctg
    22801 gcaacccgat gaaacgcgtt atgcggaaat cagtcgagaa atgctggcat ccggcgactg
    22861 gattgtgccc catctgttag ggctacgtta tttcgaaaaa cccattgccg gatactggat
    22921 taacagcatt gggcaatggc tatttggcgc gaataacttt ggtgtgcggg caggcgttat
    22981 ctttgcgacc ctgttaactg ccgcgctggt gacctggttt actctgcgct tatggcgcga
    23041 taaacgtctg gctctactcg ccacagtaat ttatctctca ttgtctatgc catcggcact
    23101 tatgccgtgc tcgatccgtt tattgcattc tggctggtgg cgggaatgtg cagcttctgg
    23161 ctggcaatgc aggcacagac gtggaaaggc aaaagcgcag gatttttact gctgggaatc
    23221 acctgcggca tgggggtgat gaccaaaggt tttctcgccc ttgccgtgcc ggtattaagc
    23281 gtgctgccat gggtagcgac gcaaaaacgc tggaaagatc tctttattta cggctggctg
    23341 gcggttatca gttgcgtact gacggttctc ccctggggac tggcgatagc gcagcgggag
    23401 cctgacttgt ggcactattt tttctgggtt gagcatattc aacgctttgc actagatgat
    23461 gcccaacata gagctccgtt ctggtactac gtgccggtca tcattgccgg tagcctgccg
    23521 tggctgggat tactccccgg tgcactgtac acaggctgga aaaaccgcaa gcattccgca
    23581 accgtctatt tgttgagctg gacgataatg ccgctgctgt ttttctccgt cgctaaaggc
    23641 aaattgccca cctatattct ttcttgcttt gcacctctgg caatgctgtt ggcgcattac
    23701 gctttgctgg cagcaaaaaa taatcctctg gcgctgcgga ttaatggctg gattaacatc
    23761 gcttttggcg tcactggcat tattgccaca tttgtgatct ccccctgggg accaatgaac
    23821 acgccggtgt ggcaaacctt cgagagctat aaagtctttt gtgcctggtc gattttttcg
    23881 ctatgggcat ttttcggctg gtacacctta acaaacgtcg aaaagacctg gtctttcgcc
    23941 gcgctttgcc cgctggggct ggcgttgctg gtaggatttt caattcctga cagagtcatg
    24001 gaaggaaaac atccgcaatt ttttgtcgag atgacacaag aatcacttca gccaagccgc
    24061 tatattctta ctgatagcgt cggtgttgcc gcaggtctgg catggagcct gcaacgcgat
    24121 gacatcatca tgtatcgcca gacaggtgag ttgaaatacg gccttaatta tccggatgcg
    24181 aaagggagat ttgtcagcgg tgatgagttc gcaaactggc ttaatcaaca tcgtcaggag
    24241 gggattatta cactcgtgct ttcggttgac cgcgatgaag atatcaacag tctcgccatt
    24301 ccgcccgcag atgccatcgt tcgtcaggag cgtctggtgc tgattcagta tcgtcccaaa
    24361 tgatctggct aacattagtc tttgccagct tgcttagcgt tgccgggcag ttgtgtcaga
    24421 aacaggcaac ctgctttgtg gcgataaaca aacggcgcaa acatatcgtg ctgtggctgg
    24481 gactggcgct ggcttgtctt ggtcttgcca tggtgctctg gctgctggtc ttgcagaacg
    24541 taccggtagg cattgcctac ccgatgttaa gtctgaattt tgtctgggtg acgctggctg
    24601 cagtaaaact gtggcacgaa ccggtatcgc cgcgtcactg gtgtggggtg gcgttcatta
    24661 ttggtggcat tgtgatcctc gggagtacgg tgtaatgggc ctgatatggg gattattcag
    24721 cgtcattatt gcctcagttg cgcaattaag cctgggtttt gcggcaagtc atctgccgcc
    24781 gatgacgcac ttatgggatt ttatcgcggc tctgttagct tttggcctgg atgcccggat
    24841 actgttactc gggctgctgg gttatctgct ttccgttttt tgttggtata aaacgctaca
    24901 taaacttgcg ctaagcaaag cctatgcctt gctcagtatg agttatgtgc tggtatggat
    24961 tgcttcaatg gtacttcccg gctgggaagg gactttttcg ctgaaagcac tactgggagt
    25021 agcctgtatt atgagcgggt tgatgctgat tttcctgccc acgacaaaac aacgttactg
    25081 agttttccct gccgctgtac actgccgttc ccactcatcg gggctataac tgcttgccga
    25141 gaggactttc accgtatgca gcttttcacg attaatacaa taaagcgcat tctgcaaagg
    25201 cgagagtaaa tcccccactt tcacggcgaa gtcactcttc acttcggcga tcatttttat
    25261 cgcgccgcca gcatcgcaga gcataagtac atgtctgtta tcttgtttat tgcagcacga
    25321 ttttttgtgc cagccattcc attgcattat cctgtttgct aagagttttc acatcaattt
    25381 acagattaac agataaactc tttatcaacg gcaatggaat ggcagaaatg agaagtgaaa
    25441 cggagggtta attattgctg acgttgcacc cactctttta gcgcctgacg tgaaatttta
    25501 ataccgccgt ttttcagctc cggcggtaaa gtcagccagc gcaccggttg ttgaaaacga
    25561 gccagcttat ctttcaccca ttcgccaaga tcaacggttt gctggtcata ctccaccacc
    25621 gctaccggcc gatgaccaaa ctccttgtcg gcaacggaaa cgataaacac ctgcaaaacc
    25681 gcaggatgtg cagcaataac acgctcgact tcttccggct gaataccctc accgccactg
    25741 aagaacagat tgtctaaacg tccgacaatg gtcagcttgc cattatgcat ctcaccgcga
    25801 tcgcgcgtag cgtaccagcc ttcgtcatta accagtgaaa ccagttgccc gttacgccag
    25861 taaccttctg ccatactggc agcccgcagc cacacttcat tattaacgat tttcacttcc
    25921 cgacccggca gcggcaaacc aacgtctgcc aggccgtcgg cttctttcgc acataccgtg
    25981 gaggcaaact cggtcagacc atagccgcaa aagcaacgaa tcccctgctc gcgcgcctgt
    26041 tccgtcaact cgaccgggat agccgcgccg ccaagtaaca ccgctttcag ggaaacggaa
    26101 ctgcggttaa ccagcaaacg ccagagttgt gttggcacca gtgaagcgtg agtacagcct
    26161 gccagcattt gctccaatgg ctgtttatca cgcaccgtca tccgcgcacc agcgtataac
    26221 cagcgccaca taattccctg accggagacg tgaaacagcg gtaaagagag caaccagtca
    26281 tcgtgatcgc caaacggaat cagcgataac acaccttgcg cactggcaag atgggcctga
    26341 taagtatgga cagcggcttt cggcaaaccg gtagaacccg aggtcaacgt cattgagcac
    26401 agacgcgtcg gctgccacgc agcggcatgt acgccttcaa ccagctgaat gtgcagcgac
    26461 gttaatgccg gaaacgtgtt ttccccatcc ggcaccagcg caaattgcag cgtcagattg
    26521 ggcagcaatt cttcaagcaa cggttgcggc agctgagggt tcacgggcaa cacccgcgcc
    26581 ccgcattgca gtaacgccag ccaggcgagc agcgtttgcg gcgtattcca cgcccgcaac
    26641 atcacgccgc tgccctcaac cacaccctgc gccgcaaagc ccgtagctaa ctcatcgacg
    26701 cgagcacaaa gctcgcgcca gttgagttgc tcgtcattaa gacgtaaggc gatggcttct
    26761 ccccgcactt gccgccagtg acgccacggc cagtcagaga agatcataac aactgctcca
    26821 gtacatcagc gtccactaac ggcagtgggc tccccggcca gcgacgaacc tgctgcgcct
    26881 gcatcagatc cagcgtgtcc agtcccggaa tggtgtctgg cgttaaccag gcagcaatcc
    26941 gcgccagttg cgttagacct aagctcgact caatggaaga actgatcacc gccgtcagcc
    27001 ccagcgcatg cgccgcctgt acctgctcgc gtactttttc cagactgccc gtgagcgtgg
    27061 gtttgataac taccgcgcgc acgccctctt cagccacaaa ggcaaaatcc ggctcgcgca
    27121 ggctttcatc ccaggcaatg gcaatgccgg tttcacgggc aaacgctcgc gaatcatcgc
    27181 gggttttgca cggctcttcg agaaacgcga tgcggtggcg ataatccggg ttaacatatt
    27241 tggcaaactg ctgacctttc agcggtgtcc aggcgcgatt tgcgtcaaga cgcaaatgca
    27301 gatccggaat tgcctccagc aacagattca ccaccatgcc gtcgcgcacc gcttcgtaca
    27361 atcccacttt gaccttcgcc actttctcgc ccggcatatc ggcaagtttg aggatcagat
    27421 cgtccggatc gccattacac agcggtgccg cacggtagtt ggctgcttgc ggcaacgtat
    27481 ctgtcagttc tgccaatgca cagcttacgc caaaggccac ggaaggcatc tgcggtagct
    27541 cgcaatcgcc tgccagccag ttatttaccc aggcaagcag cacgctttgc gcctcttccc
    27601 agttttcctg gctgaagccc ggcagtgggg agatctcccc ccacccttcg cgctcgccct
    27661 cacgcaggca aacgtacagc ccgtcgcggg tttttaaccg cctgtcgcgc agaaccaccc
    27721 ccgcgtccat ggggatctgc cagcggtata cctgcgcgct acgcattacg gattccgttt
    27781 aaatttgctg aaatcaggct gacgtttctg gttgaaagcg ttgcgacctt cctgaccttc
    27841 ttccgtcatg tagaacagca tggtggcgtt gcccgccagc tcctgcagcc ccgcctgccc
    27901 gtcacagtcg gcgttcagcg cagctttcag gcagcgcagc gccatcgggc tgttttgcag
    27961 catttcgcgg caccaacgga cggtttcttt ttccagatcc gccagcggta ccacggtgtt
    28021 gacaaggccc atatccagcg cctgttttgc gtcgtactga cggcacagga accagatttc
    28081 tcgcgctttt ttctgcccga cgatacgagc catgtaggaa gcgccccagc cgccgtcgaa
    28141 ggaaccgact ttcgggccag tctgaccgaa gatggcatta tctgctgcga tagtcaggtc
    28201 gcacatcatg tgcagaacgt gaccaccgcc gatggagtag ccagccacca tcgcgacaac
    28261 cggtttcgga caggtacgga tctgacgctg gaagtccagc acgttcaggt gatgtacgcc
    28321 ggaatcatct ttatagccgc cgtaatcacc acgcactttc tggtcaccac cggagcagaa
    28381 cgctttatcg cctgcgccag tcagaatgat cacgccgata ttgtcgtcat aacgcgcatc
    28441 tgccagtgcc tggatcatct cttttaccgt cagaggacgg aaggcattgc gcacctgcgg
    28501 acgattaatg gtgatttttg cgataccgtc ggtggatttt tcataacgaa tgtcttcgaa
    28561 accttcggag cagtcgtgcc attcaaccgg tgcgtaaggc attgcttcat caggataaat
    28621 catattgtgt cctttagtca aaaacgcaag atctgcgcca gacttgcgat tacgccagcg
    28681 ggattttccc gatgcgcgtt atgtccggcg cgaggaatga catggcagtc ggcagccagt
    28741 tccgccgcca gggcgcggaa tttgctgtca cgttcaccac ataaataata aaacgcaaat
    28801 gtgcgggcgc taaggttagc acgtaaatca ggctggacgg cgagagaagt cgcctccagc
    28861 atggcggcaa gggttgcgcc attattgttg ctgcgcagcg ccaccagctc ccggcgttga
    28921 tcgtcattga gcgaggcaaa aacaggctgt tgataccagt cggcaaatac cgccgttaac
    28981 ggttctgtgc gaaaacgctg cgcccattgg cgatcggaac gctgacgttc cgcacgttgt
    29041 tcagcatttt gcagccccgg atgcccgcct tcgataataa ccccacaaag ccccgccacc
    29101 ccctggcaag ccgccatcat cgccacccgt ccgccaagcg agtaccccac cagccagaag
    29161 tcaaggatgt tgtaactaac caaggtttta cgcagtaagt cggtgacatc atcaaatcca
    29221 tcgacgctaa tcgccgccga accaccatga cctgggagat caacatacaa ccgtgagtag
    29281 tcggcaaacg cctcgcccac ttcttgccat tcgtggcaat cgccggaaaa accgtgcaaa
    29341 aacaccagcc agggtaaacc tggttttccg tgttttgcct gcgcgtgcag gatcataaat
    29401 ggcttacctg cgccagaagt tgctggagcg tttgcgcacc atcggtgtcg ttaaccacca
    29461 tttcaatcac cgtggtggtt ggcgtgcgcc aggcgtcggc aaatgccgtt tcaagttcct
    29521 gccagttttg cggacgatga tatttcagct cgaacatcgc ggcggcgtgc tcaaaatgga
    29581 cgttttgcgg catcagatag aaacgctcac gctcgctttg cggcgttggc aacagcgaga
    29641 aaatttgccc gccgttgttg ttcaccacaa ttaataccag cggcgcagaa acctgacgca
    29701 ataacgccag cgcgttgaga tcgtaaagtg cggagagatc gcccacaatc gccagcgtcg
    29761 gtttgccgct tgcccgctga acgcctgcgg cggtcgaaag cagtccgtcg ataccgctgg
    29821 cccctcggtt gctgtacacc gggtaacctg ccggaagttg cgaaagcgca tcaatcagac
    29881 gtaccaccag gctgttacca acaaacaatt gcccctgttc aggcagatag tcgctgatgc
    29941 gatgcgccag ttgcgcttcg ccaaacgcat cacggcgggc aataaccgcc tgcattgcct
    30001 gttccgccag gcgcgggatt tcaacgcacc agggctggcg tttttctgcc ggatgcagct
    30061 ccagccagtc ggcaatattg ccaattaagc gacgtccgcg atggtgtgcc ggatcaagtc
    30121 gcccttcaat gtcatcaaca atccagtact cttctggttc acagcttgcc tgccattgca
    30181 ggagccgttt ccccgtcagg ctgcttccca gttgcaccac aatttgtgcc tgctgcagct
    30241 cgctggtcgc tttggcattg cctaaccaaa gatcggcaca cggcagcggc tgcccggttt
    30301 gtgacagcac atcgccaatc agcggccagc caagagtttg cgcccacagg gcaacttttt
    30361 tgccctcttc cgcactcatg cgcccggcaa ccaccacgcc gcgcttttgt cgccagaaga
    30421 accagtcgcg ctgtttttca ctttccagac gaggcgcttc acgcagccac ggtttgtcgt
    30481 cctgccacca gtcacccaga cgctgttgcc agctaagccc ggtatcgtcc atttcgccat
    30541 acagcggttc agcaaacggg cagttgatat ggactccccc cgcatgaagc gtaccgagag
    30601 cgtggtcgat ggtagaaacc agccaacgtg cggggatatc ctgggtcggg cgcggcaatg
    30661 aaatactgtg cgtggggtga gaggcgaaca ttcccggctg gcgaattgcc tgattcgcgc
    30721 cgcagtcaat tagctccggc gggcgatcgg cggttaagag aatcagtttt tctccggtta
    30781 acccggcttc aatcagtgcc ggatagagat ttgccaccgc cgtgccggag gtcacaatca
    30841 ccgccaccgg ctgcttgctg actttcgcca gccccagcgc cagatgcccc aacccacgct
    30901 catcgaaatg ggtgtggtga atgaatgcgg aattctccgc cgccgctaac gttaacggtg
    30961 tagaacgcga gcctggggcg atacagatgt gtctgacgcc gtgacgcgtt aatgcttcca
    31021 gaatgaccgc cgcccagcgt cggttaaatg cgcttactga catgagtttg tccggtatca
    31081 atattgcggc taagtataag gagctacaaa aatagattat tgatatgaat cggtaatgat
    31141 gcgactcatt actattccat ttgtaataaa gtacgcagcc ctgccgcttt gttgtcgatt
    31201 tcctgccact cttgctcggg gtcggaacca cggacaatgc ccgcgccagc atataatcgc
    31261 acgacattgc cgctaatttt tgctgagcgc agggaaacgc agaattcgct ttgttgtaat
    31321 gagagatagc ccgcagaacc ggcgtaccat tctcgggtga acggttcgtg acgggcgata
    31381 aactgtcgcg ccagatcgcg cggtaagcca gcaactgctg ccgttggctg caactgatgt
    31441 aaacagatca catcatccgc tttgttgagt gaagtccaga tacagcggcg aagatgctgc
    31501 actttacgca gacgcagtac ctgcggcggt aaaacatcca gcgtctgggt atcggcctgt
    31561 aatcgttggc agatatcttc caccaccagc atgttctcgc gctggttttt atcatccgcc
    31621 atcagcctct ctcctaactg ctgcgcctgc ttatcatcag gatgatttgc tactgttccc
    31681 gccagcgctt cagtacgcag cgctttgtca cgccgccgcc ataaccgttc cggtgaagag
    31741 ccaagaaaag cattttcgcc atcaaaggcc atgtaaaaat ggtagcaatt cagattcagt
    31801 cgacgactgg cagccatcat cgccgccgcg ttgaccggac ttgcgaaatg caggtcagtt
    31861 gcccgagcga gcaccacttt gtcgagctca cctttggcga tggttttcgt tgccagttcg
    31921 attaattgcg tccagcccgt tttgtccggc cagtgttgtt ctcgcgtggt ggttaaatgt
    31981 aacccaggca agggcttgat actcaccagt gtggcgataa attcttttgc ctgaatcgca
    32041 tcgtgctgaa gggagctttc gctgaatagc gtcaaccgca gcgtggcttt accgccacag
    32101 cgtcgccatt ccaggcgggg taaaagtaaa ttgccctgcg acgggtcaaa agcattcagc
    32161 ccccaaatgc gtaagtcggc gtgttccggg tgctggcgaa gaaaacgttg tgcctggtcc
    32221 aacgacgtaa aacgggtaat cgcgcccagg acggcagctt cttcatcacc attacgttgt
    32281 tgccagtaga attgcgggta cgtttgctga ctggccagcc agctcaaggc atcaaaagcg
    32341 tctttgagag ggaaaggaat atcgataacc cgaatgccgg gtgtcgccgg aatctcttgc
    32401 gacaaatggc gcagtagatt ttccagcgcc gtagtaagtg attgcacgcg gacctctccc
    32461 tgttaaaagc ctcacattat acggggtact acaaaaaaaa tgcagtaccc cggtgtaggg
    32521 aggttttaac ggcgtgccag caacagtcct agtaccagcc cgacggccgc gcccacacca
    32581 attccttgcc agggtttttc gtggacgtag tcatcagcac gataaacagc ctgcttcgcc
    32641 cgatagtaat aactgtctga agcctggcta acccgttttt tcacatcatc cagcgctttt
    32701 tctgcacgcg ctttcagctc aacatatttc tgatcggcgg gctcgccaga ggagcggagc
    32761 acctcttcca gtgtttcact aagcagcgtc aggtcgtcat cgatacgtgt atcaccaaac
    32821 tgattagaca ttctcgttct ccatttgcgt aaaacctgtc actaactata gacaagagtt
    32881 gttgattacg cctgaattac ctcgcgtgcc atcccaatgt gtgggataca atcttcttca
    32941 tagacctccg tcacagggat aaaaccgaaa ctctggtaga agttttgcag atgcgcctgc
    33001 gccccgaggt aaacaggctt atcaggccag tgatgcgtac atgtttcgag tgttttactc
    33061 atcagctgct gccccacttt ttcaccacgc aacgcttcac tgacaatcac ccgacctata
    33121 acgaccggct caagatcatc atcacttttc agaatcctcg catacgccac cagttcatca
    33181 tttttccaac cgagaatatg gcgattatcc cccgtcaaat catcaccatc gatatcctga
    33241 taagggcaat tctgctcgac aacaaacacc gcgcaacgca gttgtaataa ggcataaagc
    33301 tgggaaaccg ataattcaga gtgatgcaga tcttgccatt caatcataat gctctccttt
    33361 tagatgtatg ccttatacta gccggaatat tttttgaaac gtgatgaact catggaatta
    33421 atttttttag gtacttcagc cggtgtgcca acccgcacgc gcaatgtcac ggcaatattg
    33481 cttaacctgc aacatccgac ccagagcgga ctttggttgt ttgactgcgg tgaaggcacc
    33541 cagcatcagc tactgcatac cgcctttaac cctggaaaac tggacaagat ttttatcagt
    33601 caccttcatg gcgatcatct ttttggttta cccggcttgc tgtgcagtcg ttctatgtca
    33661 ggcattatcc aacccttaac gatttatggt ccgcaaggta tccgtgaatt tgtggaaacc
    33721 gcgctgcgga ttagcggctc atggaccgat tatccgctgg aaattgtcga aattggcgct
    33781 ggcgaaattc tcgatgatgg cctgcgcaaa gtaaccgctt atccgctgga acacccactg
    33841 gaatgttatg gctatcgtat tgaagaacat gataaaccgg gtgcattaaa tgcccaggca
    33901 ttaaaagctg ctggcgtgcc gcctggccca ctgtttcagg aattaaaagc gggcaaaaca
    33961 atcacgctgg aagatggaag gcagattaac ggcgcagatt acttagctgc tccagtgcca
    34021 ggtaaagcgc tcgctatttt cggcgatacc ggcccctgcg atgccgcact tgacctggct
    34081 aaaggtgtcg atgtcatggt gcacgaagcg acgctggata taaccatgga agccaaagcc
    34141 aatagtcgcg gccatagctc tacacgccag gctgcgacac tagcccgtga ggctggagtc
    34201 ggcaagctaa tcattaccca cgtcagctcg cgctatgatg acaaaggttg tcaacacctg
    34261 ttacgtgaat gcaggtcaat tttcccggcg actgaactgg cgaatgattt caccgtgttt
    34321 aacgtttaac tctgcttatt attaaaaaca gggtgaaact tgccctgtta tagcaacccg
    34381 cgctacatat atccacccac acaggcatat tgagcgattt ttgatattta ttcatctcct
    34441 tacgcactat attcagagca ataagttgcc tcttttcatc ttgattcagc acaacctttg
    34501 caactaaaag gagcccatga tggttacagt tgtcagcaat tattgtcaat tatctcaaac
    34561 ccaactcagt cagacatttg cagaaaaatt tactgtgacc gaggaattac tgcagtcttt
    34621 aaaaaaaaca gcgttatccg gagatgaaga aagtattgag ttactgcata atattgcgtt
    34681 aggttatgat gaatttggaa aaaaagctga agatattctt taccatattg ttagaaaccc
    34741 aacaaatgag acctatcgat tatccgactt attaaaaatg cctgtttaaa attatataat
    34801 ttggcacata ccgcaaccaa ctcccacctt aaaccaactg gtccggataa ttcagatgtt
    34861 ctcctgttca aaaagctatt ctccccttcg aaattaatga caattatcgg taatgaaatt
    34921 cctcttatat ctgaaaaaca gtcgctttca aaggtgcttt taaatgatga gaataatgaa
    34981 ctgagtgatg gtacaaactt ctgggataaa aatcgtcaat taaccacaga tgaaatagct
    35041 tgctatcttc agaagatcgc cgctaatgca aaaaatactc aagtcaatta tcctactggt
    35101 ctctacgtcc cctactccac cagaactcac ctggaagacg ctctcaatga aaatattaag
    35161 agcgatccat catggccgaa tgaagtccag t
//
LOCUS       DAJTYG010000035        35142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTYG010000035 DAJTYG010000000
VERSION     DAJTYG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.035"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(259..1287)
                     /gene="epmB"
                     /locus_tag="OO568_001808"
     CDS             complement(259..1287)
                     /gene="epmB"
                     /locus_tag="OO568_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6641180.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1329..1895
                     /gene="efp"
                     /locus_tag="OO568_001809"
     CDS             1329..1895
                     /gene="efp"
                     /locus_tag="OO568_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6641181.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1947..2072
                     /gene="ecnA"
                     /locus_tag="OO568_001810"
     CDS             1947..2072
                     /gene="ecnA"
                     /locus_tag="OO568_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6641182.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            2183..2329
                     /gene="ecnB"
                     /locus_tag="OO568_001811"
     CDS             2183..2329
                     /gene="ecnB"
                     /locus_tag="OO568_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6641183.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2505..2734
                     /locus_tag="OO568_001812"
                     /pseudo
     CDS             2505..2734
                     /locus_tag="OO568_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2731..3264)
                     /gene="blc"
                     /locus_tag="OO568_001813"
     CDS             complement(2731..3264)
                     /gene="blc"
                     /locus_tag="OO568_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6641184.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3353..4486)
                     /locus_tag="OO568_001814"
     CDS             complement(3353..4486)
                     /locus_tag="OO568_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6641185.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWIHVPPPEKKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            complement(4549..4908)
                     /gene="frdD"
                     /locus_tag="OO568_001815"
     CDS             complement(4549..4908)
                     /gene="frdD"
                     /locus_tag="OO568_001815"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6641186.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4919..5314)
                     /gene="frdC"
                     /locus_tag="OO568_001816"
     CDS             complement(4919..5314)
                     /gene="frdC"
                     /locus_tag="OO568_001816"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6641187.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5325..6059)
                     /gene="frdB"
                     /locus_tag="OO568_001817"
     CDS             complement(5325..6059)
                     /gene="frdB"
                     /locus_tag="OO568_001817"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6641188.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6052..7860)
                     /gene="frdA"
                     /locus_tag="OO568_001818"
     CDS             complement(6052..7860)
                     /gene="frdA"
                     /locus_tag="OO568_001818"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6641189.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTCLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            8185..9162
                     /gene="epmA"
                     /locus_tag="OO568_001819"
     CDS             8185..9162
                     /gene="epmA"
                     /locus_tag="OO568_001819"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6641190.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEANIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9381..10883
                     /gene="yjeM"
                     /locus_tag="OO568_001820"
     CDS             9381..10883
                     /gene="yjeM"
                     /locus_tag="OO568_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCR6641191.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11034..14345)
                     /gene="mscM"
                     /locus_tag="OO568_001821"
     CDS             complement(11034..14345)
                     /gene="mscM"
                     /locus_tag="OO568_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6641192.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALRSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(14367..15335)
                     /gene="psd"
                     /locus_tag="OO568_001822"
     CDS             complement(14367..15335)
                     /gene="psd"
                     /locus_tag="OO568_001822"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6641193.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            complement(15432..16484)
                     /gene="rsgA"
                     /locus_tag="OO568_001823"
     CDS             complement(15432..16484)
                     /gene="rsgA"
                     /locus_tag="OO568_001823"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6641194.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16579..17124
                     /gene="orn"
                     /locus_tag="OO568_001824"
     CDS             16579..17124
                     /gene="orn"
                     /locus_tag="OO568_001824"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6641195.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17335..17410
                     /locus_tag="OO568_001825"
     tRNA            17335..17410
                     /locus_tag="OO568_001825"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17368..17370,aa:Gly,seq:gcc)
     gene            17568..17643
                     /locus_tag="OO568_001826"
     tRNA            17568..17643
                     /locus_tag="OO568_001826"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17601..17603,aa:Gly,seq:gcc)
     gene            17679..17754
                     /locus_tag="OO568_001827"
     tRNA            17679..17754
                     /locus_tag="OO568_001827"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17712..17714,aa:Gly,seq:gcc)
     gene            17987..18040
                     /locus_tag="OO568_001828"
     CDS             17987..18040
                     /locus_tag="OO568_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641196.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(18023..19162)
                     /gene="queG"
                     /locus_tag="OO568_001829"
     CDS             complement(18023..19162)
                     /gene="queG"
                     /locus_tag="OO568_001829"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6641197.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            19161..20708
                     /gene="nnr"
                     /locus_tag="OO568_001830"
     CDS             19161..20708
                     /gene="nnr"
                     /locus_tag="OO568_001830"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6641198.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSVYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            20680..21141
                     /gene="tsaE"
                     /locus_tag="OO568_001831"
     CDS             20680..21141
                     /gene="tsaE"
                     /locus_tag="OO568_001831"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6641199.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            21160..22497
                     /gene="amiB"
                     /locus_tag="OO568_001832"
     CDS             21160..22497
                     /gene="amiB"
                     /locus_tag="OO568_001832"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6641200.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVLVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            22507..24354
                     /gene="mutL"
                     /locus_tag="OO568_001833"
     CDS             22507..24354
                     /gene="mutL"
                     /locus_tag="OO568_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6641201.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            24347..25297
                     /gene="miaA"
                     /locus_tag="OO568_001834"
     CDS             24347..25297
                     /gene="miaA"
                     /locus_tag="OO568_001834"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6641202.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            25383..25691
                     /gene="hfq"
                     /locus_tag="OO568_001835"
     CDS             25383..25691
                     /gene="hfq"
                     /locus_tag="OO568_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6641203.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            25767..27047
                     /gene="hflX"
                     /locus_tag="OO568_001836"
     CDS             25767..27047
                     /gene="hflX"
                     /locus_tag="OO568_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6641204.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            27133..28392
                     /gene="hflK"
                     /locus_tag="OO568_001837"
     CDS             27133..28392
                     /gene="hflK"
                     /locus_tag="OO568_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6641205.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            28395..29399
                     /gene="hflC"
                     /locus_tag="OO568_001838"
     CDS             28395..29399
                     /gene="hflC"
                     /locus_tag="OO568_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6641206.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            29481..29678
                     /locus_tag="OO568_001839"
     CDS             29481..29678
                     /locus_tag="OO568_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6641207.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            29782..31080
                     /gene="purA"
                     /locus_tag="OO568_001840"
     CDS             29782..31080
                     /gene="purA"
                     /locus_tag="OO568_001840"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6641208.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            31285..31710
                     /gene="nsrR"
                     /locus_tag="OO568_001841"
     CDS             31285..31710
                     /gene="nsrR"
                     /locus_tag="OO568_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6641209.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLLE"
     gene            31749..34190
                     /gene="rnr"
                     /locus_tag="OO568_001842"
     CDS             31749..34190
                     /gene="rnr"
                     /locus_tag="OO568_001842"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6641210.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            34370..35101
                     /gene="rlmB"
                     /locus_tag="OO568_001843"
     CDS             34370..35101
                     /gene="rlmB"
                     /locus_tag="OO568_001843"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6641211.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPMIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT         8231 a         8940 c         9623 g         8348 t
ORIGIN      
        1 attattggtg ttagctatat aaaaagtaac aacagcaatg catatgaata attaaaggtt
       61 atgcaacgtg caaagattaa atttctcagt ttaatgacag gttgctgtac ttgcgtttgt
      121 tgctcttgcc ggatgcggcg taaacgcctt atccggccta cagttcattg caaatccatt
      181 caatttcaat gcacgatgta ggcctgataa gcgtagcgca tcaggcaatt ttaatgttta
      241 acttccctgt ttaatcagtt actgctggcg tagctggaga tccagcggcg ttttgctggg
      301 ttcgccgcca atttctcgcg ccagtttcgg caccagatat cccgacacca atgtcagcaa
      361 ctcacgcata atctgccgtg cttcgtcatc actcaccata aaatgcgccg cgccctgtac
      421 tttatctagc acatgcaggt aatacggcat cacgccggca tcgaacaacg cattactcag
      481 gtttgccaac gtttgtgcgt tatcgttcac accacgtaac agaacgctct ggttaagcag
      541 ggtgacaccg acacggcgca atttagccat cgcctgacgg aacgtttcat ctacctcatt
      601 ggcatgattg atgtgattca ccagcaagat ttgcagcgta gaacgggcaa agcgttcaac
      661 cagcgcctcg gtgatacgtg ccgggatcac aatcggcaga cggctgtgaa tccgcagacg
      721 tttgatatgc gggatggctt ccagttgtgt gagcaaccag tccagctcgt gatctttcgc
      781 catcagcgga tcgccgccgg agaaaatcat ctcgtccagt tccggatgcg cagcaacata
      841 ctcaagtgca gtctgccagt tacgcttgtt gccctgattt tcggcatagg ggaagtggcg
      901 acggaagcaa tagcggcaat ttaccgcgca gccgcctttg accagcaaaa gcgcccggtt
      961 gtggtattta tgcaacaaac caggcactac gctgtgctgt tcttccagtg ggtcggtgga
     1021 gaatccggac gcgacgacaa actcatcttg cgaggtaagt acctgacgca aaagaggatc
     1081 gtccggattg cctttctcca tgcgatcgat aaatgagcgg ggcacacgca gggcaaacag
     1141 ctttttggcg ctgcgtccgg ccaacagttt ttcgtccgcg tctatattca aaagacgcag
     1201 aagttcatca ggatcggtca caacatcggc aagttgcgtt aaccaatctt ctctggatgg
     1261 ggtatttagg gttacaatat gcgccatttt gtggcttagc taccaattaa caaatttcag
     1321 agggccttat ggcaacgtac tatagcaacg attttcgtgc tggtcttaaa atcatgttag
     1381 acggcgaacc ttacgcggtt gaagcgagtg aattcgtaaa accgggtaaa ggccaggcat
     1441 ttgctcgcgt taaactgcgt cgtctgctga ccggtactcg cgtagaaaaa accttcaaat
     1501 ctactgattc cgctgaaggc gctgatgttg tcgatatgaa cctgacttac ctgtacaacg
     1561 acggtgagtt ctggcacttc atgaacaacg aaactttcga gcagctgtct gctgatgcaa
     1621 aagcaattgg cgacaacgct aaatggctgc tggatcaggc agagtgtatc gtaactctgt
     1681 ggaatggtca gccgatctcc gttactccgc cgaacttcgt tgaactggaa atcgttgata
     1741 ccgatccggg cctgaaaggt gataccgcag gtactggcgg caaaccggct accctgtcta
     1801 ctggcgctgt ggttaaagtt ccgctgttcg tacaaatcgg cgaagtcatc aaagtggata
     1861 cccgctctgg tgaatacgtc tctcgcgtga agtaatgcgg ttgtggtgcg gcctgcaggc
     1921 tgcaccatca cttattcagg tcagagatga tgaaacgcct tatcgttctt gttttgcttg
     1981 ccagcacgct gctcacgggc tgtaacaccg cttgcggttt cggcgaagac atcaaacatc
     2041 tcggcaactc catctctcgc gctgccagct aatttttctt ctcttccgaa aaatcatcag
     2101 atttccatca tttttggcga tgttgtctat tattaatttg ctataggcaa acataaataa
     2161 cattacctaa aaggaagacg ttatggtgaa gaagacaatt gcagcgatct tttctgttct
     2221 ggtgctttca acagtattaa ctgcctgcaa caccacgcgt ggcgttggtg aagacatttc
     2281 tgatggcggt aacgcgattt ctggcgcagc aacgaaagcg cagcaataag caataacggt
     2341 acgacagcag tgtcgtgccg tttgtttttt ctgcgatcgt cataaaggta atagttgaaa
     2401 ttcccctgcc acctggcaaa atatccgttc aaccatcagc tttgcaggac gacctgcaaa
     2461 cgcctctttt caccggggac ggccccaaat ttccggagcc tgatatgtcc tggattatct
     2521 tagttattgc tggtctgctg gaagtggtat gggccgttgg cctgaaatat acccacggct
     2581 ttagccgttt gatgccgtgt ggacgggtat tggcgcagtc ggcgcggcca tcaccggcat
     2641 tgtgctgctc ggtgagtccg ctaacccgat gcgtctggcg agtctggcgt taatcgtatt
     2701 ggggattatt ggtctgaaac tcagcactca ctaactaccg ggctgctgta cccaaataaa
     2761 cttactgaca tcaaaccctt cccgggtcgc gactgccagc atctcctgtt tcacttcgtc
     2821 agaaatggtt ggcgtgcggg agagtatcca caggtagtcg cggtccgggc cgcaaaccag
     2881 cgcatggcgg tattcccgat cgagtgcaat aacgttataa ccgccataga aaggaccaaa
     2941 gaatgacact ttcagcgcag cgcgagttgg tgcgccggta aagtacgctt tcccttcact
     3001 ctgctgccac attcctctgt cagggttata gcctttatta atgacattca ggccgccgtc
     3061 atcacgcagg ctgtatgttg cggtgacttt ttccagtcca cgttcaaaac ggtgatcaaa
     3121 acgggcaatc tcataccagg taccgagata acgtttggcg tcgaaattat tcactacggt
     3181 cacgccacgc ggcggcgtag gagaactaca ggcaacgacc agaaatgcag ctgtcgctgc
     3241 ggcaacgaga gggagcaggc gcataaatgt ttccttactg gtttttttct aagtgtagat
     3301 gacagcaagg aaaagcgcag aaaaggtccg aaaattcgga cccgatgaaa ttttactgta
     3361 gagcgttgag aatctgacag gcggcggcga ctctctctgg attgggatag tttttgttag
     3421 ccagcatcac gatacccagc tctttttctg gaataaacgc gacatagcta ccaaatccgc
     3481 cggtcgcccc cgttttatgt acccatgatg cgcgtactgc aggagttggg ggcttaatcg
     3541 cttttaccgg gcgtgctgcc agtgcaattt tattgtcact gccgttaatg atgctgtcag
     3601 gattgaccgg ccagtccagc atttcccagc ccaggccctg atacatatcg ccggtttgcc
     3661 agtagcgaga ttgtgccagt tgtatccctt gttgaagcgt tttctcattg atatcaaggg
     3721 ggggtaaatt gctttgcacc cagcgggcca tatcttcaat ggtcgacttc acaccataag
     3781 cttcagcatc taacgcccct ggcgaaacat gcactgcctt accttcgcga tatccccagg
     3841 cgtaattctt tttttctggg ggaggtacat gaatccacgt atggttgagt ttgagtggct
     3901 ggaagacacg agtttgcatc gcctgctcaa aactcaaacc agacggcttc acagccagtg
     3961 cgccgaacaa accgatactg gagttggcat acagacgttg tgttcctgga gcccatgcag
     4021 gctgccagtt ttgatagaag cgcagcaagt cgcttgagga tttcacttca tccggcacct
     4081 gcaatggcag gccgccagcg gtgtaggtcg caagatgtaa tagtgtgatc ccattccact
     4141 gtttagcggt aagttcaggc cagtattttg ttgcgggatc gcttaacttg atttcccctc
     4201 gagcaatagc gtcgccacca agcacgcccg taaatgtttt gctgaccgaa cctaactcaa
     4261 acaacgtttg ctgtgtgacg ggctgctttt tggcgatgtc cgcatagccc caggtaaagt
     4321 aataaggttt accctgataa attaccgcca ccgccatacc ggggatcttt tgttgctcta
     4381 taagcggggt aattgtgcga tgcacaatat cgttgatttg ttgaggggca gcaaatgtgg
     4441 agcaagaggc ggtaattaat aaggcgcaga gcgtcgtttt gaacataagg tctggtttcc
     4501 atacaaaacg gcccgccata ggcgggccgg atttacattg gcgatgcgtt agattgtaac
     4561 gacaccaatc agcgtgacaa ctgtcaggat agcagccaga ccgtagaaaa cccatttgcc
     4621 cgcaggcacg tggattttca gatcgtgcat cgcgtggtgc atacggtgta aaccacacca
     4681 cagcggcaga acgatcatca ggaacaggaa tacgcgacca atgaagctct gcgcgaacgc
     4741 cagaacgcgc tcgtagctca gcgcatcacc cggaaacagc cccagtggca gcagaatacc
     4801 caccagcagg atcatcaccg gcgcaataat tgcgctccac ataccaccgg ccccgaagag
     4861 gccccagaat accggttcgt cagaacgctt tggatttgga ttaatcatct caggctcctt
     4921 accagtacag ggcaacaaac aggattacga tggtggcaac cacagttacc gcccagagac
     4981 ttttgataat tggctctggt cccatttttt cgtcttttac aatgatattg gccgctttcg
     5041 gtgccagttc aaaccaggtt ttggtgtgca gcagagctgc cgtcagagtg atcaggttaa
     5101 tgatcacgat aaccgggttt tgtaaaaagt cgacgaatcc cgcccaggct tccgggccat
     5161 ttttcagggc aaacagcccg aaaatcagtt caatgctgaa ccacacagcc ggaaccgccg
     5221 tgccttcgcg cagcatgtaa aagcgataaa acggcaattt tttccaccag gtggacgtca
     5281 ttggccgtac atacggttta cgtttagtcg tcatgttgca ctccttagcg tggtttcagg
     5341 gtcgcgataa gaaagtcttt cgaactttct actttgccct gctgaatggc cgcagccgga
     5401 tcgacgtgtt tcgggcagac ttcggagcaa tagcccacga aagtacagct ccatacaccg
     5461 ttctggctgt tcaactgcgc catacgctcc ttcttaccgt ggtcgcggct atcttcgtta
     5521 taacgatgcg ccagcgtaat ggcagccgga ccgatgaact ctgggttcag gccaaactgc
     5581 gggcacgcgg cgtagcacag accacagttg atgcaaccgg agaactggtg atactttgcc
     5641 atctgcgccg gggtctggat gttagtaccc tgatccgcgg tgcgggagtt gccgatgatg
     5701 tacggtttga tcgcttccag actttcgatg aagtgggtca tatcgaccac cagatcgcgt
     5761 tcaatcggga agttagctaa cgcttcaacc ttcataccgt cggtgtaatc acgcaggaag
     5821 gttttacatg ccagttttgg cacgttgtta accatcatgc cgcaggaacc acaaatcgcc
     5881 atacggcagg accagcggta actcaggtcc ggtgccaggt tgtctttgat gtagcccagc
     5941 gcatccagta atgaggtagt tgcgtcataa ggcacttcat agaatgcgct atgcggtgcg
     6001 gtatcgactt ccgggttata gcgcaccacc tcaattttca ggtttttcat ctcagccatt
     6061 cgccttctcc ttattattgg ctgcttccgc cttatcggct gcatccgctt cgccaccgta
     6121 aacgcgttta gctggcggca gcgtagtaat cttcacgtcg ctgtactcca ggcaagtcgt
     6181 gccatcagca tcgcggaagg cgagggtgtg tttgagaaag ttgacgtcgt cacgctcggt
     6241 gcaaccttcg tccagacgct ggtgtgcgcc gcgggactct ttacgtgcca ttgcggagtg
     6301 cgccatacat tcagcaacgt tcagaccgtg gctcagttca atggtgtaga gcaggtcggt
     6361 gttgaacacg ctggaggtgt cggtgatgcg cacgcgcttg aagcgttcct gcagctctgc
     6421 cagcttgtcg atggttttct gcatcagttc cggcgtacgg tagataccgc aaccttcttc
     6481 cattgccagg cccatttcgt cgcggatctt cgcccagttt tcgccgccat cctggttaac
     6541 cagatctttc agacgttgtt caacgccagc tgcctgcgct tcaattgccg cttcgttgcc
     6601 attaccggca gttgctgcac gctctgtcgc ttgttcaccg gccagacggc cgaagaccac
     6661 cagttccgcc agggagttgg agcccagacg gtttgcaccg tgcagaccaa cagaggaaca
     6721 ttcacccacg gcgaacagac ctttaatgcg ggtttcacag ttctgatcgg tttcgatacc
     6781 gcccatggtg tagtgtgcag tcggacgtac cggaatcggt tctttaaccg gatcgacgcc
     6841 aacgtacgct ttcgccagtt cgcagatgaa cggcagacgt tcatgcagtt ttttctcgcc
     6901 gaggtgacgc aggtcgagat aaaccacatc accacgcggc gtggagatgg tgttgccttt
     6961 acgccattcg tgccagaagg cctgagaaac tttgtcgcgt ggacccagtt ccatatattt
     7021 gtttttcggc tcgcccagcg gagtttccgg gcccatgccg taatcttgca gataacggta
     7081 gccatttttg ttgaccagaa taccgccttc accacggcag ccttcggtca tcaggatacc
     7141 ggaacctggc agaccggttg ggtgatactg aacgaattcc atgtcacgca gcggaacgcc
     7201 gtggcttagc gccataccca taccgtcacc ggtaacgatg ccgccgttgg tgttgtaacg
     7261 ataaacgcga cccgcaccgc cggtagccat aacgaccgcg ttagcacgga tctgcaccaa
     7321 cgtgccttcc atcatgttca ttgctaccag gccgcgaaca tgaccatcat caaccagaat
     7381 atccagcacg aaatgttcgt caaaacgctg gatctgcggg aattgcagag aggtctggaa
     7441 cagcgtgtgc agcatatgga agccggtctt atcggcggcg aaccaggtgc gctcgatttt
     7501 catgccgccg aagcgacgta cgttgacgct accatccggg cgacggctcc atgggcatcc
     7561 ccacagttcc agttgggtca tttcggttgg gcagtggtgg acgaaataat ccacgacatc
     7621 ctgctcacac aaccagtcgc cacccgctac tgtatcgtga aagtgatatt cgaagctgtc
     7681 atgatcctgc gcgacagcgg cggagccccc ttctgcagca acggtatggc tacgcatcgg
     7741 gtatactttt gagattagtg cgatttttgc atttggattt gcctgcgcgg cagcaattgc
     7801 agcacgtaat cccgcgccac cggcgcctac aatggcaaga tcggcttgaa aggtttgcac
     7861 gacattcctc cagattgttt ttatcccaca gccccgtact tcagggtaag tgcctgaaag
     7921 ttacggtctg cgaacgctat tccactgctc ctttataggt acaacagtat agtctgatgg
     7981 ataagtctga aatttgacga gatcgatttt tttagtgcgc aagagggcta aattatcact
     8041 gaagatgatt aatttaatta ctaaaccatc agatcgtgct ttttttagtc actgcccacc
     8101 gctgtttgat tcctgcgtaa aatttgtctc gccgtcgcgt tgcgagtaga cttcgtgccc
     8161 ttgtcaaaaa ctggagattt aactatgagc gaaacggcat cctggcagcc gagcgcatcc
     8221 attcctaact tattaaaacg cgcggcgatt atggcggaga tccgtcgttt ctttgccgat
     8281 cgtggagtgc tggaggtgga gacgccttgt atgagccagg cgacggtaac cgatattcat
     8341 ttggtcccgt ttgagacacg tttcgttggc cccgggcatt cgcaggggat gaatctctgg
     8401 ttaatgacca gcccggaata ccatatgaaa cgcctgctgg ttgctggttg tgggccggta
     8461 ttccagctgt gccgcagctt ccgtaatgaa gagatggggc gttatcacaa ccctgagttc
     8521 actatgctgg agtggtatcg accgcactat gatatgtacc ggttgatgaa cgaggtggac
     8581 gatctcttac aacaggtgct ggactgcccg gcagcagaaa gcctttctta tcaacaagct
     8641 ttcttgcgtt atctggaaat tgacccgctc tctgccgaca aaacgcaact gcgggaagtc
     8701 gcagcgaaac tggatttgag caatgttgct gataccgaag aagaccgcga cacgttgcta
     8761 caattgctgt ttacctttgg cgtagaggca aatattggta aagaaaaacc gacctttgtg
     8821 taccactttc cagccagcca ggcatcactg gcgcaaatca gtaccgaaga tcatcgggtc
     8881 gctgaacgct ttgaggttta ttataaaggt attgagctgg cgaatggttt ccatgaatta
     8941 acggatgccc gtgagcagca acaacgcttt gaacaagata accgtaagcg cgcggcgcgc
     9001 ggtttgccgc agcaccccat tgaccagaat ctgattgaag ccttgaaagt cggtatgcct
     9061 gactgctccg gcgtggcatt aggtgttgat cgtctggtga tgttggcgct gggcgcggag
     9121 acactggctg aagtcatcgc ctttagcgtt gaccgggcat aattctgaaa aattactgaa
     9181 ttaacagcga agaatggcgt gataagcgcc attctgtagc atatgttttc atttttccgt
     9241 taaaggccac aatttaagac tggatatttg atatcatcaa ggtatcaatt ctgtattggt
     9301 ttttacgctg cctgctcaat gttgcgcagt gcagttcagt gggcacgtgt tatacacgcg
     9361 ctgaaatgaa ggatggtttc atgcctcaca cgataaaaaa gatgagtctg ataggactca
     9421 tattgatgat ctttacttcc gtatttggat ttgccaatag cccatcggct tattacttaa
     9481 tgggttatag tgcgattccc ttttatatat tttctgcatt gttattcttt attccattcg
     9541 ccttaatgat ggctgaaatg ggagctgctt atcgcaaaga agaaggtggt atctattcct
     9601 ggatgaataa tagtgtcgga ccacgttttg ccttcattgg tacgtttatg tggttttcct
     9661 cttatatcat ctggatggtg agtacctccg cgaaagtttg ggtaccgttc tcaacattcc
     9721 tctatggtag tgacatgacc cagcactggc gtattgccgg actggagcct acgcaggtgg
     9781 ttggtctgct ggcagtagca tggatgattc tggtcaccgt cgttgcttca aaggggatta
     9841 ataaaattgc ccgcattact gcggtgggcg gtattgcagt aatgtgtctg aatttagtat
     9901 tgctgttagt aagcattact attttgttat taaatggtgg gcatttcgcg caggatatta
     9961 atttccttgc atcaccgaac ccgggttatc agtccggtct ggcaatgcta tcgtttgtgg
    10021 tatttgctat ttttgcctat ggcggaattg aagcggttgg tggtctggtc gataaaacgg
    10081 aaaatccaga aaagaacttt gccaaaggta ttgtttttgc cgctattgtt atttcaatcg
    10141 gttattcgct ggcaatattt ttatggggcg tcagcacaaa ctggcagcag gtattaagta
    10201 atggttccgt taaccttggc aatattacct atgtgctgat gaagagcctc ggggtgacgc
    10261 tgggtaacgc actgcatttg tcacctgaag cgtcattgtc gctgggcgta tggtttgcgc
    10321 gtattaccgg actttcgatg ttcctcgctt ataccggtgc gttctttacg ctttgctatt
    10381 caccgctgaa agccatcatc caggggacgc cgaaagcgtt gtggccggaa ccgatgacgc
    10441 gcctgaatgc gatggggatg ccgtctatcg ccatgtggat gcagtgcggg ttggttactg
    10501 tcttcattct gctggtttcg tttggtggcg gtaccgcatc ggcgttcttt aacaagctga
    10561 cgctgatggc gaacgtgtct atgacgcttc cttacctgtt cctcgcgctg gctttcccgt
    10621 tttttaaagc acgtcaggat ctcgacagac catttgtgat tttcaaaacg cgtatgtcgg
    10681 caatgattgc gacggtggtt gtcgttctgg tggtgacatt tgcgaacgtc ttcaccatta
    10741 ttcagcctgt ggttgaagcc ggagactggg acagcacatt gtggatgatt ggcggccctg
    10801 tcttcttctc gctgttagcg atggcgattt accagaacta ttgcagtcgc atggcgaata
    10861 aacctgagtt agctctcgac tgatcatatg aaatcctgca actcatcgtc tctccggcga
    10921 tgagttgtca ggaagtaatg cgtatggatg atactctcca gggccggtga taactgaaga
    10981 tgatgttaag ctgctaaatg agccacgttg acatcatttg gcagcttcat gcattacaaa
    11041 cttcccgcct gacgaccttt gcccgcagac gtcagcgttc tccccgtttg tttaccgttg
    11101 aggctttcca gacgcatctg gaagggcggg aatggcatat cgataccgtg ggcgtggaag
    11161 cctgccagaa tcagctggtg gatctcatgg cgtagcggca tacggtgacc catctcagcg
    11221 gcgtaaatac gcagctcgaa aatctgaatc ccctgttgca gatccaccag gaagacttcc
    11281 ggtgccgggt tgtcgatcac cagcgagcag cgacgcgctg cggtgagcag gatttccgtc
    11341 acttcttcac tgttggcatc ggcaggggcc ggaatcgtca acactacgcg cgtgaccgag
    11401 tcagagagcg accagttgat aaactgctcg gtaataaacg ccttgttcgg cacgattatc
    11461 tctttacggt cccagtcgct gatggtggtg gcgcgggtgt taatttttgt cacgctacca
    11521 gtaagatcgc ggatcgttac cgtatcgcca atgcgaatcg gtttttcgaa caggatgatc
    11581 aggccagaga taaagttggc gaaaatttcc tgcaaaccaa aaccgagacc aacaccgagc
    11641 gcggcaacca gccactgcaa tttcgaccac tcaataccaa tcattgagaa gccgaccagc
    11701 ccgccaatca gcatcagcag atattttgtg atggtggtga tggcgtaacc cgtacccggc
    11761 gttaaatcca ggtgctgcaa aatcgccagt tccagcagcg cgggcaggtt gcgcaccagc
    11821 tgcgtggtga tgataaatac cagaatggca atcagcaccg caccgagggt aattggctcc
    11881 agactttcta cgccctgtac cgtggaggtg acatcccaca gcgaaatatt ttcgaggaag
    11941 ccgaaagcgg aatggatttc tgaccacagc acaatgaccg aacgcagggc gatcaacatc
    12001 aaaattgagc gcaccagccg caaggattgc gcactgatgg catcgagatc gacttcgctt
    12061 tcatcgactt caattgctcc ttccgggcta ctgtgatgat gcgcttcctc ttcgccacgc
    12121 gcacgttgcg ctaacatctc tgcccggcga tgcttcgccc ggtcaaacgc cagcctgcga
    12181 cgctggatca gcatccagcg gcggataacg tgataaacca ccagtagcag gaaccagatg
    12241 gcaaccgagg tttcaagcct cgctaacagt gcctgcgccg ttgccagata acccaccgcc
    12301 gacgccagaa tggcaaccag tggtgcgcca atcatcatgt tccacagcat atggttggta
    12361 atgttgtcgc cgctgccttc tttgttgaga tacaacggaa taccggcttt tttcaggctt
    12421 agggtgacca ccgccagcgc accgcaaatg aggataaagc aaagccgtcc cagcgaaccg
    12481 gaaaattcgc ggtcgtcgag gttatcgaac atcatcagcg ccataatcag cccgatgctc
    12541 atcaggtagt agcgcatccc acgggaaaca cgttcgcgcg gccagccaaa atgagcgata
    12601 aacaagccgt tcgggcgggc aaaggtggcg caaatcatca ctacccacag cagcggcacg
    12661 gtggccgtta caccatcgcc aatcgcgacc gccagcggat aaggccacgc ctcgcgcaag
    12721 ccgtaaccca gcgtcatcca cagcaccggt aacggtgacg cgacgagaat cgaccagaaa
    12781 agagtgcgca acgtcagcca gaagtgatcc tgagtcactt tgccgacttt cgccgccgaa
    12841 cgttcaagaa aacgggtgaa atagcggcgc gagtaaatac tgcaaccgac cagaatcaac
    12901 gcgccaaaca gcggcaaaat tgtctctttg ctggtcagca tcatcacact ggctttgccc
    12961 aactgactga aggtgtccag cgaaatgaga cgacgcagat cctgggcgat ttccagcggc
    13021 caggcgatgg tcatcgggcg cacgtcagag gtccagaaca gatagcggtg cgttgcttcg
    13081 ttcacctctt tcagcgcatc ctccagttgc ccgttggaga ctttcagctt ggtcagttcc
    13141 aatagtagcg tgtcgccacc ctgcaacaat gagttcagca actcacgctg agtgcgcagt
    13201 tgtgcttcca gaatacggtt ttgctcggcg gtcagcggct gaccgtcggc ctgatggatt
    13261 tgccgtagca gcggctgttt attaagtaaa tcctcataac gtaaccgttg cacacgcaac
    13321 tgcgccattt cggtatcaag ctgttgtggt tttggcattt ctggtagccg tgccacctgc
    13381 gcccgcagcg cttcgccgag caggttggac gatcccagcc attgcgactg ttcacgcagc
    13441 gtattcaacg cctgccggac ctgtaacgtc tggctggcag cctgacgctg ttgcgaggca
    13501 acgagctcca tccgctgcgc ctgttgattc agcgctgccg atagttcgcg gttaattttg
    13561 aattgcgcga cgatatcttt cggcaaatcg gcactgtttt ctgccagtaa ttcggtactt
    13621 tccagcgccc gctccgcctc aagctgacgt tggctattca actgattacg taagccctgc
    13681 aaatacgcat ccagttgctg gctctctttt tccgccagct ctgagcgtaa gcgcgctaat
    13741 tcctggcggt tattggcaga cagctgcgcc agctccagtt catcaacgag cgccttaaga
    13801 cgtgcagagt cagactgcaa cgcgaaattt tgtgcctgat tgagcggagt attgccggta
    13861 agcgttccca ggcggcgctc gatctcattt aactgacggc gggcgtcggt ttgctgttgc
    13921 ggcagttgat tcagcgaatc ggcaatctcg cgggcgcgct cctgctcttg ctgggcctga
    13981 cggcttttat ccagcaactg gctgctgacc tggagaattt cctgattcag cgcgtcggta
    14041 gacattcccg gcgacacgct gcgcggctcg tcacgcatgt tgtttaattg tgcgcgcaga
    14101 gtagcggaga gtttcggata attatcgata acttgctgat attgtttgat gcgctcaagg
    14161 gaaccttttc gttcctcaag cgcatttaag gcagactgga gcgcctctac gacttccggc
    14221 tgtgcgggtt tcgccgcttt tgcctgctcc agttcctgag tgatttgttt gctatcgggg
    14281 gccgtcgcgg cgtacgcccc ccaactgagg caccaggcca tcagaaaagt gataatcagg
    14341 cgcacgtcag cgtttccttt gatggattag acctggtctt ttttatcgtc aaccaatggc
    14401 ctggcgtcgt gttctgcttc gatctcttca gcaggaagtg gggcgggttc agcgtctggc
    14461 gtaacaaagg tttcggtaga tactgccagc ggctggccaa ttttcgtgac agacaggctt
    14521 tccagttgct caaccagatt cactttaccc ggtgcaaaca ggttgataac ggtggaaccg
    14581 agtttaaagc gacccatttc ctggcctttc agcagtgcca cagaaccgtc gttttccccg
    14641 gcaggccagg tccagcgctt gatgatacct tcgcgcggcg gcgtaatggt gcccgcccag
    14701 accgtctcaa tgctgccaac aatcgtcgct ccgaccagaa tctgcgccat tgggccaaat
    14761 tcggtatcga aaaggcaaat cacgcgttcg ttacgggcaa acagattcgg cacgttctga
    14821 gcggtgagat ggttaacgga aaagagatcg cccggcacgt agatcatctc acgcagaata
    14881 ccgttgcacg gcatgtgtac gcgatggtag tcacgcgggg agaggtaagt ggtcacaaac
    14941 gtaccgttgc ggaacaggtc cgccatcaga tagttgcctg ccagcagggc ttcgaggctg
    15001 tagttgtggc ctttggcttg caggatttta tcttcttcga ttttacccaa ctggctgata
    15061 acgccatcgg caggcatgac cagtacattc ggatcggtat cgattgggcg tacttcgtca
    15121 cgcagcggac ggacaaagaa ttcgttaaag gtgcggtagc tggcggtgtc cggcttttgc
    15181 gcctctttca tgtcgacctt gtagtattta acgaacagat cgataaccag ttttgtcagc
    15241 catcctgccc gcttgcttgc gccccaaccc gccaggcgag taagccatag tttcggcaga
    15301 atgtactgta gcgaaagttt aaatgaattt aacaaggtag cctccaggcc attgttttgt
    15361 cgttcctgat ccggccaaca tgccggatcc tgaaaaaaag gggacgattc taacgacggt
    15421 tagcttaatt gtcagtcatc cgtatcagaa aagtttttac gcgtttttac ctgcgccatg
    15481 ctttccagaa tacggtgata gttttcgaaa cgggtttccg cgattttccc ttcctcaacc
    15541 gcttcccgga tagcgcagcc cggatcggta tcgtgtttgc aatcgcgata tttacacaga
    15601 cctaaatagt catggaattc gacaaagccc tgagtgattt gttccggctc caggtgccag
    15661 aggccgaact cacgcacacc aggggaatca atcacatcac cgccgtgcgg gaagtgatac
    15721 agccgagcgg cggtcgtggt gtgctgaccg agccccgagt tgtcagagac atcgttggtc
    15781 aggatctctt tttgcagccc cagcaacgcg ttcagcaggc tggatttacc gacgccagac
    15841 tgcccggcaa aaatgctgat gcgcccggtc aacgcctctt ccagcggttt cagcccatcc
    15901 tgagtatggc tggaaaccat caatacgcga taaccgatgt tgcggtagat atccatctgc
    15961 tcgttgacga acgccatgcc ttcgtcgtcc agcaggtcta tcttgttgag cacgataatc
    16021 ggctcaatct gcaaggtttc gcaggccacc aggtaacggt cgataatatt gagcgacagc
    16081 tccggcaaaa tggcggagac aatgacaatc tggtcgatgt tggcggctat gggtttcacg
    16141 ccgtcgtaga aatccggacg cgttaacacc gaggtgcgct catgcaccgc ttccacgatc
    16201 cctttgacat ttacgccttc cgccgccggt ttacccggac gccagactac gcggtcgccg
    16261 gttaccagcg aacggatggt acggcgaata ttgcagcggt gaacgtcgcc atcggcggat
    16321 tccacatcag cgtgcatacc aaagcggctg atgacgatac cttcatcagg ctcgccaaac
    16381 agattgtcgt cgtagtcggg cttctcctta gacgttttaa gacgacgctg gtgattggcg
    16441 ttcacgcggc gctgctggcc tttggagagt ttatttttac tcaatcgtac tgactcctgg
    16501 tcgcccctga tgggcaaaac atctatgata cacgcaattg tggatcaata tagtcactgt
    16561 gaatgggtgg aaaatagcat gagtgccaat gaaaacaacc tgatttggat cgatcttgag
    16621 atgaccggtc tggatcccga gcgcgatcgc attattgaga ttgccacgct ggtgaccgat
    16681 gccaatctga atattctggc agaagggccg accattgcag tacatcagtc cgatgaacag
    16741 ctggcgctga tggatgactg gaacgtgcgc acccataccg ccagcgggct ggtagagcgc
    16801 gtgaaagcga gcacgatggg cgatcgggaa gctgaactgg caacgctcga attcttaaaa
    16861 cagtgggtgc ctgcgggaaa atcgccgatt tgcggtaaca gcatcggaca ggaccgtcgt
    16921 ttcctgttta aatacatgcc ggagctggaa gtctacttcc actaccgtta tctcgatgtc
    16981 agcaccctga aagagctggc gcgccgctgg aagccggaaa ttctggatgg ttttaccaag
    17041 caggggacgc atcaggcgat ggatgatatc cgtgaatcgg tggcggagct ggcttactac
    17101 cgcgagcatt ttatcaagct gtaaaatttt tcctggtcac gtaagcgccg gaatccactt
    17161 gcggcggcgt gattttgacg ctaaatcgaa cgtcttgctg ataatttcag cacttgagat
    17221 aaaaacgcaa aaaaaacttt tttggggggt tgcagaggga aagatttctc gtataatgcg
    17281 cctcccgtaa cgacgcagaa atgcgaaaat tacgaaagca aaattaagta gtacgcggga
    17341 atagctcagt tggtagagca cgaccttgcc aaggtcgggg tcgcgagttc gagtctcgtt
    17401 tcccgctcca aaatttgaaa agtgctgcaa agcacagacc acccaatcgc agtatttcga
    17461 attgcgacaa ggcggcaact gaacgaatcc ttgggaactt acaaaagtat gtgactaagg
    17521 tgagtgaagg cagccaaggc agtagcggtt cgaaagacga agattaagcg ggaatagctc
    17581 agttggtaga gcacgacctt gccaaggtcg gggtcgcgag ttcgagtctc gtttcccgct
    17641 ccaaaatttg aaagtgctgt aaggcacaga ccacccaagc gggaatagct cagttggtag
    17701 agcacgacct tgccaaggtc ggggtcgcga gttcgagtct cgtttcccgc tccaaattct
    17761 tctctcaata aaatatccac agcgacgcga tgcgttattg ctggtttttg ttgtctctga
    17821 caaactcttg taaacagagt tatccacagc ctcaggctgt aatcttaatt tcaaagaaac
    17881 ttcgcacggt gaatggtatt ttttaaccta ttgataaata agttaaaaat caagattccg
    17941 ttttgtcgag tcgatcactt gacgatttta ttcgtcttga attgcaatgc gtttttattt
    18001 ttattcacaa gctgtggatg aatcaggcgt cacgcggtaa ccctttttca atcacccgaa
    18061 ccagacgctg ttttttcggc aattgcactt cgactatgca cgcatttcgt ctctctattt
    18121 gctgcgcaat cgcccacgct atgtgctcat cgagaagtgg gtgctcacct ttacgacttt
    18181 ccagcgctgt caaaatcgtt tcatcccaag gggcattgcc taatgccacg gcgatattac
    18241 gcagccagcg caggtgacct atacggcgaa tcgccgatcc ttccgtgact tttaaaaact
    18301 tctcttcgct ccaggcaaat aactcaatga gttccggtgc gtgtagcggc ttacgcgggc
    18361 tgaaatcgtc ttctgtagtg agttgcgaat agcgattcca cgggcagata agctggcagt
    18421 catcgcaacc gtaaatacgg tttcccatta acggtcgcaa ctcttccggg atcgcccctt
    18481 caagttcgat ggtgagataa gagatacagc ggcgagcatc gacggtatat ggctcgacga
    18541 tggcaccggt cgggcaaatc gtcatacagg ccacgcattt gccgcatcct tcctcgactg
    18601 gttgatccac gggcagcgga atatcgacca gcaattcgcc taaaaagaag aacgaaccgg
    18661 cctcgcgatt gaggataagt gagtgcttac ctgtccagcc gagcccagct ttttcagcta
    18721 acgggcgctc gagaataggc gcagaatcga caaacggtct aaaattcagc gaaacacaat
    18781 gttgctgaat catctcgccc agctttttga gtcggttgcg cagaagtttg tgatagtcac
    18841 ggcccagcgc ataacggcta acatagccga gtttggggtt tttcagcgtg ctggcaaatg
    18901 cggcgttagc aggaaggtaa ttcatccgca cgctgattac acgcagcgta ccgggtaata
    18961 actcatgggg gcgagcgcgc agcataccgt gacgtgccat ccagtccatt tcgccgtggt
    19021 attgtttgtc cagccatgct tgcagtttgg gctcggactc gctgagatcg gtatcggtaa
    19081 tacctacctg ctgaaagccc agttccagcc cccactgttt aattttttgc gctaactgat
    19141 tgagatcgag gggctctgac atgacggacc atacaatgaa gaaaaacccc gtaagtatac
    19201 cacacaccgt ctggtacgcc gacgatatcc gccgcggaga acgcgaggcg gcagatgtgc
    19261 tggggctcac actctatgag ctgatgcttc gcgctggcga ggccgcattc caggtgtgtc
    19321 gttcggtgta tcctgacgcc cgccactggc tggtgctgtg cggtcatggt aataacggcg
    19381 gcgatggcta cgtggtcgcg cgactggcca aagcggtcgg cattgaggtc acgttgttgg
    19441 cccaggagag cgacaaaccg ttgccggaag aggccgcgct ggcacgcgaa gcatggttaa
    19501 acgcgggtgg cgagatccat gcttcgaata ttgtctggcc cgaatcggta gatctgattg
    19561 ttgatgcgct gctcggtacc ggcttgcagc aagcgccccg cgaatccatt agccagttaa
    19621 tcgaccacgc taattcccat cctgcgccga ttgtggcggt tgatatccct tccggcctgc
    19681 tggctgaaac cggcgctacg ccaggcgcag tgatcaacgc cgatcacacc atcactttta
    19741 ttgcgctgaa actaggcttg ctcactggaa aagcgcggga tgttacagga caactgcatt
    19801 ttgactcact ggggctggat agctggctgg caggtcagga gacgaaaatt cagcggtttt
    19861 cggcagaaca actttctcac tggctgaaac cgcgtcgccc gacttcgcat aaaggcgatc
    19921 acgggcggct ggtgattatc ggtggcgatc acggcacggc gggggctatt cgtatgacgg
    19981 gggaagcggc gctacgtgct ggtgctggtt tagtccgagt actgacccgc agtgagaaca
    20041 ttgcgccgct gctgactgca cgaccagaat tgatggtgca tgaactgact atggactctc
    20101 ttaccgaaag cctggaatgg gccgatgtgg tggtgattgg tcccggtctg ggccagcaag
    20161 agtgggggaa aaaagccctg caaaaagttg agaattttcg caaaccgatg ttgtgggatg
    20221 ccgatgcatt gaacctgctg gcaatcaatc ccgataagcg tcacaatcgc gtgatcacgc
    20281 cgcatcctag cgaggccgca cggttgttag gctgttccgt cgctgaaatt gaaagtgacc
    20341 gcttacattg cgccaaacgt ctggtacaac gttatggcgg cgtagcggtg ctgaaaggtg
    20401 ccggaaccgt ggtcgccgcc catcctgacg ctttaggcat tattgatgcc ggaaatgcag
    20461 gcatggcgag cggcggcatg ggcgatgtgc tctctggtat tattggcgca ttgcttgggc
    20521 aaaaactgtc gccgtatgat gcagcctgtg caggctgtgt cgcgcacggt gcggcagctg
    20581 acgtactggc ggcgcgtttt ggaacgcgcg ggatgctggc aaccgatctc ttttccacgc
    20641 tacagcgtat tgttaacccg gaagtgactg ataaaaacca tgatgaatcg agtaattccg
    20701 ctccctgatg agcaggcaac attagacctg ggcgagcggg tagcgaaagc ctgcgatgga
    20761 gcaaccgtaa tctatctgta tggcgattta ggcgcaggta aaaccacctt tagccggggc
    20821 tttttacagg ctctgggtca tcagggtaat gtcaaaagcc ccacttatac gctggtcgaa
    20881 ccctatacgc tcgacaactt aatggtctat cactttgatt tgtaccgcct tgccgatccc
    20941 gaggagctgg agtttatggg gatccgcgat tattttgcca acgatgccat ctgccttgtg
    21001 gagtggccac aacaaggtac aggtgttctt cctgacccgg atgtcgaaat acacattgat
    21061 tatcaggcac aaggccgtga ggcgcgcgtg agtgcggttt cctctgcggg tgaattgttg
    21121 ctggcgcgtt tagccggtta acctttgaaa ggtggcggga tgatgtatcg catcagaaat
    21181 tggttggtag cgacgctgct gctgctgtgc acgccggtgg gtgccgcgac gctctctgat
    21241 attcaggttt ctaacggtaa tcaacaggca cggataacgt tgagttttat tggcgatcct
    21301 gattatgcgt ttagccatca aagcaaacgc accgtggcgc tcgatatcaa acaaacgggc
    21361 gtgattcagg gactgccgtt gttgttcagc ggcaataatc tggtgaaggc gattcgctct
    21421 ggaacgccta aagatgcaca aacgctacgg ctggtggtcg atcttaccga aaacggtaaa
    21481 accgaagcgg tgaagcggca gaatggcagc aattacactg tcgtctttac gattaacgcc
    21541 gatgtgccgc caccgcctcc tccgccgccc gtggttgcga aacgcgttga aacgcctgcg
    21601 gttgtcgcac cgcgcgtcag cgaaccggcg cgcaatccgt ttaaaacgga aagtaaccgc
    21661 actacgggtg ttatcagcag taatacggta acgcgtccgg cagcgcgcgc gacggctaac
    21721 actggcgata aaattatcat cgctattgat gccggacacg gcggtcagga ccctggcgct
    21781 atcggccccg gtggtacgcg ggagaaaaat gtcaccatcg ccatcgcgcg taaattgcgt
    21841 actttgctca atgacgatcc gatgtttaaa ggcgttttaa cccgtgacgg ggattacttt
    21901 atctcggtga tggggcgcag tgatgtggca cataagcaaa acgccaattt cctcgtgtcg
    21961 attcacgctg atgccgcacc gaaccgcagt gcgactggcg cttccgtatt ggtgctctct
    22021 aaccgtcgcg ccaacagtga aatggccagc tggctggagc agcacgagaa acagtcggag
    22081 ctgctgggtg gggcgggtga tgtgctggcg aacagtcagt ctgaccccta tttaagccag
    22141 gcggtgctgg atttacagtt cggtcattcc cagcgggtag ggtatgatgt agcgaccagt
    22201 atgatcagtc agttgcaacg cattggcgaa atacataaac gtcgaccaga acacgccagc
    22261 cttggcgttc tgcgctcgcc ggatatccca tcagtactgg tcgaaaccgg ttttatcagc
    22321 aacaacagcg aagaacgttt gctggcgagc gacgattacc aacaacagct ggcagaagcc
    22381 atttacaaag gcctgcgcaa ttacttcctt gcgcatccga tgcaatctgc gccgcagggt
    22441 gcaacggcac aaactgccag tacggtgacg acgccagatc gcacgctgcc aaactaagga
    22501 cgattgatgc caattcaggt cttaccgcca caactggcga accagattgc cgcaggtgag
    22561 gtggtcgagc gacctgcgtc ggtagtcaaa gaactagtgg aaaacagcct cgatgcaggt
    22621 gcgacgcgta tcgatattga tatcgaacgc ggtggggcga aacttatccg cattcgtgat
    22681 aacggctgcg gtatcaaaaa agatgagctg gcgctggcgc tggctcgtca tgccaccagt
    22741 aaaatcgcct ctctggacga tctcgaagcc attatcagcc tgggctttcg cggtgaggcg
    22801 ctggcgagta tcagttcggt ttcccgcctg acgctcactt cacgcaccgc agaacagcag
    22861 gaagcctggc aggcctatgc cgaaggacgc gacatggacg tgacggttaa accggcggcg
    22921 catccggtgg gaacgacgct ggaggtgctg gatctgttct acaacacccc ggcgcggcgc
    22981 aaattcctgc gcaccgagaa aaccgaattt agccatattg atgaaatcat ccgccgcatt
    23041 gcgctggcgc gtttcgacgt cacgatcaac ctgtcgcata acggtaaaat tgtgcgtcag
    23101 taccgcgcag tgccggaagg cgggcaaaaa gaacggcgct taggcgcgat ttgcggcacc
    23161 gcttttctcg aacaagcgct ggcgattgaa tggcaacacg gcgatctcac cctgcgcggc
    23221 tgggtggccg atccaaatca cactacgccc gcactggcgg aaattcagta ttgctacgtg
    23281 aatggtcgca tgatgcgcga tcgcctgatc aatcatgcga tccgccaggc ctgcgaagac
    23341 aaactggggg ccgatcagca accggcattt gtgttgtatc tggagatcga cccgcatcag
    23401 gtggacgtca acgtacaccc cgccaaacac gaagtgcgtt tccatcagtc gcgtctggtg
    23461 catgatttta tctatcaggg cgtgctgagc gtgctacaac agcaactgga aacgccgcta
    23521 ccgctggacg atgaaccaca acccgcaccg cgtgccatcc cggaaaaccg cgtggcggcg
    23581 gggcgcaatc actttgcaga accggcagct cgtgagccgg tagctccgcg ctactctcct
    23641 gcgcccgcct caggcagtcg tccggctgcc ccctggccga atgcgcagcc aggctaccag
    23701 aaacagcaag gtgaagtgta tcgccagctt ttgcaaacgc ccgcgccgat gcaaaaacca
    23761 aaagcgccgg aaccgcagga acctgcactt gcggcgaaca gtcagagttt tggtcgggta
    23821 ctgactatcg tccattccga ctgtgcgttg ctggagcgcg atggcaacat ttcactttta
    23881 tccttgccag tggcagaacg ttggctgcgt caggcacaat tgacgccggg tgaagcgccc
    23941 gtttgcgccc agccgctgct gattccgttg cggctaaaag tttctggcga agaaaaatcg
    24001 gcattagaaa aagcgcagtc tgccctagcg gaattgggta ttgatttcca gtcagatgca
    24061 cagcatgtga ccatcagggc cgtgccttta cccttacgcc aacaaaattt acaaatcttg
    24121 attcctgaac tgataggcta cctggcgaag cagtccgtat tcgaacctgg caatattgcg
    24181 cagtggattg cacgaaatct gatgagcgaa catgcgcagt ggtcaatggc acaggccata
    24241 accctgctgg cggacgtgga acggttatgt ccgcaacttg tgaaaacgcc gccgggtggt
    24301 ctgttacaat ctgttgattt acatccggcg ataaaagccc tgaaagatga gtgatatcag
    24361 taaggcgagc ctgcctaagg cgattttttt gatggggccg acggcctccg gtaaaacggc
    24421 gttagccatt gagctgcgta aaattttacc agtagagttg ataagcgttg attctgccct
    24481 tatttacaaa gggatggata tcgggacggc gaagccgaac gctgaagagt tactcgccgc
    24541 gccgcaccga ttgctggata ttcgcgatcc gtcgcaggct tactcggctg ctgattttcg
    24601 ccgcgatgcg ctggcggaaa tggccgatat caccgcggcg gggcggatcc cactgttagt
    24661 gggcggtacg atgttgtatt tcaaggcatt gctggaaggg ttgtcgccgc taccgtcggc
    24721 agacccggaa gtgcgggcca gaattgagca acaggcggca gagcaaggtt gggagtcatt
    24781 gcatcgtcaa cttcaggagg tagatccggt tgcggcagca aggattcatc caaatgatcc
    24841 acaaaggctt tcccgggcac tggaagtttt tttcatttcg ggtaaaactt taacggaact
    24901 gacgcaaacg tcaggagacg ctctaccgta tcaggtgcat cagttcgcca tcgccccggc
    24961 gagccgtgaa ctgctccatc aacgcattga gcagcgtttt catcagatgt tggcttcagg
    25021 ttttgaagca gaagtccggg cgctttttgc ccgaggagat ttgcatacgg acttgccttc
    25081 cattcgttgc gtgggttatc gccagatgtg gtcttacctt gaaggcgaaa tctcatacga
    25141 tgaaatggtt tatcgaggtg tttgcgccac gagacagttg gcgaagcggc agataacctg
    25201 gctgcgtggt tgggaagggg ttcactggct tgacagtgaa aaaccagaac aggcgcgtga
    25261 cgaagtatta caggttgttg gtgctatcgc aggctgaatg tgtacaattg agacgtatcg
    25321 tgcgcaattt tttcagaatc gaaaggttca aagtacaaat aagcatataa ggaaaagaga
    25381 gaatggctaa ggggcaatct ttacaagatc cgttcctgaa cgcactgcgt cgggaacgtg
    25441 ttccagtttc tatttatttg gtgaatggta ttaagctgca agggcaaatc gagtcttttg
    25501 atcagttcgt gatcctgttg aaaaacacgg tcagccagat ggtttacaag cacgcgattt
    25561 ctactgttgt cccatctcgc ccggtttctc atcacagtaa caacgccggt ggcggtacca
    25621 gcagtaacta ccatcatggt agcagcgcac agaatacttc cgcgcaacag gacagcgaag
    25681 aaaccgaata aggtttcggg ctgttttttt acacggggag ccagcgatcc tgcgttcccc
    25741 gctgatctat ttagagggtt atacgcttgt ttgaccgtta tgatgctggt gagcaggcgg
    25801 tactggtaca catctatttt acgcaagaca aagatatgga agacctccag gagtttgaat
    25861 ctctggtctc ttccgccggt gtcgaagcat tgcaggtgat taccggtagc cgtaaagcgc
    25921 cgcacccaaa gtattttgta ggtgaaggta aagcagttga aattgcggaa gctgtcaaag
    25981 cgacgggtgc ttcggtcgtt ctttttgacc atgccctgag cccggcgcaa gagcgtaacc
    26041 tggagcgttt gtgcgagtgt cgtgttatcg accgcaccgg ccttatttta gatattttcg
    26101 cccaacgtgc gcgtacccat gagggtaagt tgcaggttga gctggcgcag ctgcgccatc
    26161 tggctacgcg cctggtgcgt ggctggaccc accttgaaag acagaaaggc gggataggtt
    26221 tgcgtggtcc gggtgaaacc cagctcgaaa ccgaccgtcg tttgttgcgt aatcgcatcg
    26281 tgcagataca gtcgcgcctg gaaagagttg aaaagcagcg tgagcagggg cggcaatcgc
    26341 gtatcaaagc cgacgttcct actgtttcgc tggtgggata taccaacgcc ggtaaatcta
    26401 cccttttcaa tcgcatcacc gaagcgcggg tctacgcggc agaccagttg tttgccaccc
    26461 tcgacccgac gttgcggcgt attgacgttg cagatgtcgg tgaaaccgta cttgcagata
    26521 ccgtagggtt tattcgccac ctgccgcacg atctggtggc agcatttaaa gccacgttac
    26581 aagagacgcg gcaagccaca ttactgctgc acgtcattga tgcggcggat gtgcgtgtac
    26641 aagaaaacat cgaagcggtg aatacggttc ttgaagagat cgacgctcac gagatcccaa
    26701 ccctgctggt gatgaacaag atcgatatgc tggaagattt cgaaccgcgt attgatcggg
    26761 acgaagagaa caaaccgatc cgtgtctggc tttccgcaca gaccggagcg gggataccac
    26821 agctttttca ggctttgacg gagcggcttt ccggcgaggt ggcgcagcat acattgcgtc
    26881 tgccaccgca ggaagggcgt ctgaggagtc gtttttatca gcttcaggca atagaaaaag
    26941 agtggatgga ggaggacggc agcgtaagtc tgcaagttcg tatgccgatc gttgactggc
    27001 gtcgcctctg taaacaagaa ccggcgttga tcgattacct gatctaacgg cgtagcgtct
    27061 gaagcgtgga gtcatatcct ctggcgtcga aagacaacag ggatcaccgc ataacaaata
    27121 tggagcacaa acatggcgtg gaatcagccc ggtaataacg gacaagaccg cgacccgtgg
    27181 ggaagcagca aacctggcgg caactctgag ggaaatggaa acaaaggcgg tcgcgatcaa
    27241 gggccacctg atttagatga tatcttccgc aaactgagca aaaagctcgg tggtctgggc
    27301 ggcggtaaag gcaccggatc tggcggtggc agttcatcgc aaggcccgcg cccgcagctt
    27361 ggcggtcgtg tcgttaccat cgcagcggca gcgattgtca ttatctgggc ggccagtggt
    27421 ttctatacca ttaaagaagc cgaacgcggc gtggtaacac gctttggtaa attcagccat
    27481 ctggttgagc cgggtctgaa ctggaaaccg acgtttatcg acgaagtcaa accggtgaac
    27541 gtggaagccg tgcgtgaact ggccgcttct ggtgtgatgc tgacgtcgga cgagaacgta
    27601 gtgcgcgttg agatgaacgt gcagtaccgc gtcaccaatc cggaaaaata tctgtatagc
    27661 gtgaccagcc cggatgacag cctgcgtcag gctaccgaca gcgccctgcg tggagttatc
    27721 ggtaaataca ccatggaccg cattctgacg gaaggtcgta ccgtgattcg tagcgatact
    27781 cagcgcgaac tggaagagac gattcgtccg tatgacatgg gtatcacgct gctggacgtc
    27841 aacttccagg ctgctcgtcc gccggaagaa gtaaaagcgg cgtttgacga tgcgattgcc
    27901 gcgcgtgaaa acgaacagca atacattcgt gaagcagaag cgtataccaa cgaagttcag
    27961 ccgcgtgcga acggtcaggc gcaacgtatc ctcgaagagg cgcgtgcgta caaggcccag
    28021 accatcctgg aagctcaggg tgaagtggcg cgctttgcta aacttctgcc ggaatataaa
    28081 gccgcgccgg aaattactcg cgagcgtctg tatatcgaga cgatggaaaa agtgttgggt
    28141 aacacccgca aagtgctggt taacgataaa ggtggcaacc tgatggttct gccgttagac
    28201 cagatgttga aaggtggtaa cgcccctgcg gcgaagagcg ataacggtgc cagcaatctg
    28261 ctgcgtctgc cgccagcctc ttcctccaca accagtggag caagcaacac gtcgtccacc
    28321 agtcagggcg atattatgga ccaacgccgc gccaacgcgc agcgtaacga ctaccagcgt
    28381 cagggggaat aacgatgcgt aagtcagtta tcgcgattat catcatcgtg ctggtagtgc
    28441 tttacatgtc tgtctttgtc gtcaaagaag gtgagcgcgg tattacgctg cgttttggta
    28501 aggtactgcg tgacgatgac aacaaacctt tggtttatga gcctggtctg catttcaaga
    28561 taccgttcat tgaaacggtg aaaatgctcg acgcacgtat tcagaccatg gacaaccagg
    28621 ccgaccgctt tgtgaccaaa gagaagaaag acctgatcgt cgactcttac atcaaatggc
    28681 gcatcagcga tttcagccgt tactacctgg caacgggtgg tggcgacatt tcgcaagcgg
    28741 aagtgctgtt gaaacgtaag ttctctgacc gtctgcgttc tgaaattggt cgcctggacg
    28801 tgaaagatat cgtcaccgat tcccgtggtc gtctgaccct cgaagtacgt gacgcgctga
    28861 actccggttc tgcgggtaca gaagatgaag ttactacccc ggcagcagat aacgccattg
    28921 ccgaagcggc agagcgcgta acggctgaga cgaagggcaa agttccggtc atcaacccga
    28981 acagtatggc ggcgctgggt attgaagttg tcgatgtgcg tatcaagcag atcaacctgc
    29041 cgaccgaagt gtctgaagcg atctacaacc gtatgcgcgc cgagcgtgaa gcggtagcgc
    29101 gtcgtcaccg ttcacaaggt caggaagaag cggaaaaact gcgcgcgact gccgactatg
    29161 aagtgaccag aacgctggca gaagctgagc gtcagggccg cattatgcgt ggtgaaggcg
    29221 atgccgaagc agccaaactg tttgctgatg cattcagtaa agatccggac ttctacgcat
    29281 tcatccgtag cctgcgtgct tatgagaaca gcttctctgg caatcaggac gtgatggtca
    29341 tgagcccgga tagcgatttc ttccgctaca tgaagacgcc gacttccgca acgcgttaat
    29401 ataacgactg cggtacaggt caataaagcc accgcatcct cagggatgtc ggtggttttc
    29461 tttttctata aggataatga atgaattcga caatctggct ggcgcttgcc ctggttttgg
    29521 tactggaagg tttagggccg atgctttacc cgaaggcatg gaagaagatg atctctgcga
    29581 tgaccaattt gcccgataat attttacgtc gttttggcgg tggacttgtg gttgcgggcg
    29641 ttgtggtcta ctacatgttg aggaaaacga ttggctgaac aaaaaacaga ctgttcgagg
    29701 tcatttttga gtgcaaaaag tgctgtaact ctgaaaaagc gatggtagaa tccattttta
    29761 agcaaacggt gattttgaaa aatgggtaac aacgtcgtcg tactgggcac ccaatggggt
    29821 gacgaaggta aaggtaagat cgtcgatctt ctgactgaac gggctaaata tgttgtacgc
    29881 taccagggcg gtcacaacgc aggccatact ctcgtaatca acggtgaaaa aaccgttctc
    29941 catcttattc catcaggtat tctccgcgag aatgtaacca gcatcatcgg taacggtgtt
    30001 gtgctgtctc cggccgcgct gatgaaagag atgaaagaac tggaagaccg tggcatcccc
    30061 gttcgtgagc gtctgctgct gtctgaagca tgtccgctga tccttgatta tcacgttgcg
    30121 ctggataacg cgcgtgagaa agcgcgtggc gcgaaagcga tcggcaccac cggtcgtggt
    30181 atcgggcctg cttatgaaga taaagtggca cgtcgcggtc tgcgtgttgg cgaccttttc
    30241 gacaaagaaa ccttcgctga aaaactgaaa gaagtgatgg aatatcacaa cttccagttg
    30301 gttaactact acaaagctga agcggttgat taccagaaag ttctggatga tacgatggct
    30361 gttgccgaca tcctgacttc tatggtggtt gacgtttctg acctgctcga ccaggcgcgt
    30421 cagcgtggcg atttcgtcat gttcgaaggt gcgcagggta cgctgctgga tatcgaccac
    30481 ggtacttatc cgtacgtaac ttcttccaac accactgctg gtggcgtggc gaccggttcc
    30541 ggcctgggcc cgcgttatgt tgattacgtt ctgggtatcc tcaaagctta ctcaactcgt
    30601 gtaggtgcag gtcctttccc ggccgaactg tttgatgaaa ctggcgagtt cctctgcaag
    30661 cagggtaacg aattcggtgc aactacgggt cgtcgtcgtc gtaccggctg gctggacacc
    30721 gttgccgttc gtcgtgcggt acagctgaac tccctgtctg gcttctgcct gactaaactg
    30781 gacgttctgg atggcctgaa agaggttaaa ctctgcgtgg cttaccgtat gccggatggt
    30841 cgcgaagtga ctaccactcc gctggcagct gacgactgga aaggtgtaga gccgatttac
    30901 gaaaccatgc cgggctggtc tgaatccact ttcggcgtga aagatcgtag cggcctgccg
    30961 caggcggcgc tgaactacat caagcgtatt gaagagctga ccggtgtgcc gatcgatatc
    31021 atctctaccg gtccggatcg tactgaaacc atgattctgc gcgacccgtt cgacgcgtaa
    31081 ttctggtacg cctggcagat attttgcctg ccgggctaac agtgtgatac attgctgtgt
    31141 cggttaagcc attacgctat ccgacacagt gttaaatcct cgcttttttc cttccccgaa
    31201 ctgaaataaa ttagcgacac agcttgtggc tggtttatca tcaatataaa tgtatttttt
    31261 cccgatttcc cttttgaggt tgatgtgcag ttaacgagtt tcactgatta cggattacgt
    31321 gcgctgatct acatggcgtc attgccagaa gggcggatga ccagtatttc tgaagtgact
    31381 gacgtctacg gcgtctcccg taatcatatg gtcaaaataa tcaatcaact tagtcgtgcc
    31441 ggctacgtga ctgctgttcg tggaaaaaat ggcggcattc gcctgggtaa accggcgagt
    31501 gcgatacgta ttggtgatgt ggtgcgcgag ctggagccct tatcgctggt gaattgcagc
    31561 agtgagtttt gccacattac acctgcctgt cggttgaaac aggcgctttc taaggccgtg
    31621 caaagttttc ttacggaact ggataactac acgcttgccg atttggttga agagaatcaa
    31681 ccgctttata aattattgct gttggagtga cgaaaatctt catcagagat gacaacggag
    31741 gaaccgagat gtcacaagat cctttccagg aacgcgaagc tgaaaaatac gcgaatccca
    31801 tccctagtcg ggaatttatc ctcgaacatt taaccaaacg tgaaaaaccg gccagccgtg
    31861 atgagctggc ggtagaactg cacattgaag gcgaagagca gcttgaaggc ctgcgtcgcc
    31921 gcctgcgcgc gatggagcgc gatggtcaac tggtcttcac tcgtcgtcag tgctatgcgc
    31981 tgccggaacg cctcgacctg gtgaaaggta ccgttattgg ccaccgtgat ggctacggct
    32041 ttctgcgggt tgaagggcgt aaagatgatt tgtatctctc cagcgagcag atgaaaacct
    32101 gcattcatgg cgatcaggtg ctggcgcagc cgctgggtgc tgaccgtaaa ggtcgtcgtg
    32161 aagcgcgtat tgtccgcgta ctggtgccaa aaaccagcca gattgttggt cgctacttta
    32221 ccgaagcggg cgtcggcttt gtggttcctg acgatagccg tctgagcttc gatatcttaa
    32281 tcccgcccga tcagatcatg ggcgcgcgga tgggctttgt ggtcgtagtc gaactgactc
    32341 agcgtccgac tcgccgcacc aaagcggtgg gtaaaatcgt cgaagtgctg ggcgacaata
    32401 tgggcaccgg catggcggtt gatatcgctc tgcgtaccca tgaaattccg tatatctggc
    32461 cgcaggctgt tgagcaacag gttgcggggc tgaaagaaga agtgccggaa gaagcaaaag
    32521 cgggccgtgt cgatttgcgc gatttaccgc tggtcaccat tgatggcgaa gacgcccgtg
    32581 actttgacga tgcagtttac tgcgagaaaa aacgcggcgg cggctggcgt ttatgggtcg
    32641 cgattgccga cgtcagctac tatgtgcgtc cgccaacgcc gctggacaga gaagcgcgta
    32701 accgtggcac gtcggtgtac ttcccttcgc aggttatccc gatgctgccg gaagtgctct
    32761 ctaacggcct gtgttcgctc aacccgcagg tagaccgcct gtgtatggtg tgcgagatga
    32821 cggtttcgtc gaaaggccgc ctgacgggct acaaattcta cgaagcggtg atgagctctc
    32881 acgcgcgtct gacctacacc aaagtctggc atattctgca gggcgatcag gatctgcgcg
    32941 agcagtacgc cccgctggtt aagcatctcg aagagttgca taacctctat aaagtgctgg
    33001 ataaagcccg tgaagaacgc ggtgggatct catttgagag cgaagaagcg aagttcattt
    33061 tcaacgctga acgccgtatt gaacgtatcg aacagaccca gcgtaacgac gcgcacaaat
    33121 taattgaaga gtgcatgatt ctggcgaata tctcggcggc gcgtttcgtt gagaaagcga
    33181 aagaaccggc actgttccgt attcacgaca agccgagcac cgaagcgatt acctctttcc
    33241 gttcagtgct ggcggagctg gggctggagc tgccgggtgg taacaagccg gaaccgcgtg
    33301 actacgcgga actgctggag tcggttgccg accgtcctga tgcagaaatg ctgcaaacca
    33361 tgctgctacg ctcgatgaaa caggcgattt acgatccaga aaaccgtggt cacttcggtc
    33421 tggcattgca gtcctatgcg cactttactt cgccgattcg tcgttatcct gacctgacgc
    33481 tgcaccgcgc cattaaatat ctgctggcga aagagcaggg gcatcagggc aacaccactg
    33541 aaaccggcgg ctaccattat tcgatggaag agatgttgca actgggtcag cactgttcga
    33601 tggcggaacg tcgtgccgac gaagcaacgc gcgatgtcgc tgactggctg aagtgtgact
    33661 tcatgctcga ccaggtaggt aacgtcttta aaggcgtaat ttccagcgtc actggctttg
    33721 gcttcttcgt ccgtctggac gacttgttca ttgatggtct ggtccatgtc tcttcgctgg
    33781 acaatgacta ctatcgcttt gaccaggtag ggcaacgcct gatgggggaa tccagcggcc
    33841 agacttatcg cctgggcgat cgcgtggaag ttcgcgtcga agcggttaat atggacgagc
    33901 gcaaaatcga ctttagtctg atctccagtg aacgcgcacc gcgcaacgtc ggtaaaacgg
    33961 cgcgcgagaa agcgaaaaaa ggcgatgcag gcaaaaaagg cggcaagcgt cgtcaggtcg
    34021 gtaaaaaggt aaactttgag ccagacagcg ccttccgcgg tgagaaaaaa acgaagccga
    34081 aagcggcgaa gaaagacgcg agaaaagcga aaaagccatc ggcgaaaacg cagaaaatag
    34141 ccgcagcgac caaagcgaag cgtgcggcga agaaaaaagt ggcagagtga tcaataccct
    34201 ctttaaaaga agagggttag attgctgaca aaatgcgctt tgttcatgcc ggatgcggcg
    34261 tgaacgcctt atccggccta cataatcacg caaattcaat atattgcaga gatcatgtag
    34321 gccggataaa cgtagtgcat caggcaaaac gtaaacaacg agtacattaa tgagcgaaat
    34381 gatttacggc atccacgcag tgcaggccct gctggagcgc gcccctgaac gttttcagga
    34441 agtctttatt ttaaaaggcc gtgaagataa acgtctgtta ccgctgattc acgcccttga
    34501 gtctcagggc gtggttatcc agttggcaaa ccgccaatat ctcgacgaga aaagcgacgg
    34561 tgccgtgcat cagggcatta tcgcccgcgt gaagccagga cgtcagtatc aggaaaacga
    34621 tctgccggat ctgatcgctt cgctcgatca accgttcctg ctgatcctcg acggcgtaac
    34681 cgatccgcac aacctcggcg cgtgcctgcg tagtgcggac gccgcaggcg ttcatgcggt
    34741 gattgtgccg aaagatcgct ccgcacagct caacgctacg gcgaaaaaag tagcctgcgg
    34801 cgcggcagaa agcgttccga tgattcgggt gactaacctt gcgcgcacca tgcgtatgtt
    34861 gcaggaagag aatatctgga tcgtcggtac ggcaggcgag gcggatcata cactctatca
    34921 gagcaaaatg accggacgcc tggcgctggt gatgggcgcg gaaggtgaag gtatgcgtcg
    34981 cctgacccgt gaacattgcg atgagttgat cagcatccca atggcaggaa gtgtttcttc
    35041 cctgaacgtt tcggttgcaa ccggaatttg cttatttgaa gcggttcgtc agcgcagcta
    35101 gttcctctga attatgaaaa gccatccaga tttggatggt tt
//
LOCUS       DAJTYG010000036        34266 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTYG010000036 DAJTYG010000000
VERSION     DAJTYG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.036"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(94..2190)
                     /locus_tag="OO568_001844"
     CDS             complement(94..2190)
                     /locus_tag="OO568_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6641212.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRLAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
     gene            complement(2192..2443)
                     /locus_tag="OO568_001845"
     CDS             complement(2192..2443)
                     /locus_tag="OO568_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6641213.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            2596..3159
                     /locus_tag="OO568_001846"
     CDS             2596..3159
                     /locus_tag="OO568_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641214.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            complement(3435..3857)
                     /locus_tag="OO568_001847"
                     /pseudo
     CDS             complement(3435..3857)
                     /locus_tag="OO568_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(3857..4450)
                     /locus_tag="OO568_001848"
     CDS             complement(3857..4450)
                     /locus_tag="OO568_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR6641215.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTIIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            4597..5004
                     /locus_tag="OO568_001849"
     CDS             4597..5004
                     /locus_tag="OO568_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6641216.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(5124..6116)
                     /gene="rpoS"
                     /locus_tag="OO568_001850"
     CDS             complement(5124..6116)
                     /gene="rpoS"
                     /locus_tag="OO568_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6641217.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(6179..7318)
                     /gene="nlpD"
                     /locus_tag="OO568_001851"
     CDS             complement(6179..7318)
                     /gene="nlpD"
                     /locus_tag="OO568_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6641218.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKIGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGELSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(7458..8084)
                     /gene="pcm"
                     /locus_tag="OO568_001852"
     CDS             complement(7458..8084)
                     /gene="pcm"
                     /locus_tag="OO568_001852"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6641219.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(8078..8839)
                     /gene="surE"
                     /locus_tag="OO568_001853"
     CDS             complement(8078..8839)
                     /gene="surE"
                     /locus_tag="OO568_001853"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6641220.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSSASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(8820..9869)
                     /gene="truD"
                     /locus_tag="OO568_001854"
     CDS             complement(8820..9869)
                     /gene="truD"
                     /locus_tag="OO568_001854"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6641221.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(9866..10345)
                     /gene="ispF"
                     /locus_tag="OO568_001855"
     CDS             complement(9866..10345)
                     /gene="ispF"
                     /locus_tag="OO568_001855"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6641222.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(10345..11055)
                     /gene="ispD"
                     /locus_tag="OO568_001856"
     CDS             complement(10345..11055)
                     /gene="ispD"
                     /locus_tag="OO568_001856"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6641223.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(11074..11385)
                     /gene="ftsB"
                     /locus_tag="OO568_001857"
     CDS             complement(11074..11385)
                     /gene="ftsB"
                     /locus_tag="OO568_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6641224.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(11579..11902)
                     /locus_tag="OO568_001858"
     CDS             complement(11579..11902)
                     /locus_tag="OO568_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6641225.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(11952..12557)
                     /gene="cysC"
                     /locus_tag="OO568_001859"
     CDS             complement(11952..12557)
                     /gene="cysC"
                     /locus_tag="OO568_001859"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6641226.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVAAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEEPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(12557..13984)
                     /gene="cysN"
                     /locus_tag="OO568_001860"
     CDS             complement(12557..13984)
                     /gene="cysN"
                     /locus_tag="OO568_001860"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6641227.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(13986..14894)
                     /gene="cysD"
                     /locus_tag="OO568_001861"
     CDS             complement(13986..14894)
                     /gene="cysD"
                     /locus_tag="OO568_001861"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6641228.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15146..16183
                     /gene="iap"
                     /locus_tag="OO568_001862"
     CDS             15146..16183
                     /gene="iap"
                     /locus_tag="OO568_001862"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6641229.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(16329..17420)
                     /locus_tag="OO568_001863"
                     /pseudo
     CDS             complement(16329..17420)
                     /locus_tag="OO568_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(17614..17766)
                     /locus_tag="OO568_001864"
     CDS             complement(17614..17766)
                     /locus_tag="OO568_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6641230.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(18030..18764)
                     /gene="cysH"
                     /locus_tag="OO568_001865"
     CDS             complement(18030..18764)
                     /gene="cysH"
                     /locus_tag="OO568_001865"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6641231.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(18838..20550)
                     /gene="cysI"
                     /locus_tag="OO568_001866"
     CDS             complement(18838..20550)
                     /gene="cysI"
                     /locus_tag="OO568_001866"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6641232.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(20550..22349)
                     /gene="cysJ"
                     /locus_tag="OO568_001867"
     CDS             complement(20550..22349)
                     /gene="cysJ"
                     /locus_tag="OO568_001867"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6641233.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            22668..23030
                     /gene="queD"
                     /locus_tag="OO568_001868"
     CDS             22668..23030
                     /gene="queD"
                     /locus_tag="OO568_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6641234.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            23108..24379
                     /locus_tag="OO568_001869"
     CDS             23108..24379
                     /locus_tag="OO568_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6641235.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            24370..24630
                     /locus_tag="OO568_001870"
     CDS             24370..24630
                     /locus_tag="OO568_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6641236.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GGLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            24647..25222
                     /locus_tag="OO568_001871"
     CDS             24647..25222
                     /locus_tag="OO568_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR6641237.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(25370..26230)
                     /locus_tag="OO568_001872"
     CDS             complement(25370..26230)
                     /locus_tag="OO568_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6641238.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGTEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIDVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(26227..27006)
                     /locus_tag="OO568_001873"
     CDS             complement(26227..27006)
                     /locus_tag="OO568_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6641239.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRCGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(26984..28393)
                     /locus_tag="OO568_001874"
     CDS             complement(26984..28393)
                     /locus_tag="OO568_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6641240.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(28415..29853)
                     /locus_tag="OO568_001875"
                     /pseudo
     CDS             complement(28415..29853)
                     /locus_tag="OO568_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(29923..30709)
                     /locus_tag="OO568_001876"
                     /pseudo
     CDS             complement(29923..30709)
                     /locus_tag="OO568_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            31027..32304
                     /locus_tag="OO568_001877"
     CDS             31027..32304
                     /locus_tag="OO568_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6641241.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            32331..33809
                     /locus_tag="OO568_001878"
     CDS             32331..33809
                     /locus_tag="OO568_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6641242.1"
                     /translation="MSKKYIIGIDGGSQSTKVVIYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHILFDVQMPGTVLGHITPQGALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         8275 a         9073 c         8426 g         8492 t
ORIGIN      
        1 atcatcaata tcagtcatag tatttcctcg gttatttcaa atcagctttg tttcctttat
       61 ttaacagtcc gttcccgcgg actgtttttt tattcaggcc agcggatcat cttcttcctg
      121 catggcaaaa agtttttgca tccccagccg gaaatcgcgt tcaaattctt cgtcatgctc
      181 ttcttcatat tccggttgct gaactgccac cacattacca accgggcgat aaacattact
      241 cagccagggt tcagcctgtt tgtgttccag aatctgaata ccttcatctt cagcatcacg
      301 aattttttcc tcagcgcgtt tgcgcattcc tttaatacgc tgctgttgtt tgtgatattc
      361 agcactgaca gcaaaggctg cgcgtttatt gccgttccat atcgcctcac agataaagct
      421 gccgtccttg cgacgtaccg taattcgctc ggcgtcatga atgtcataac tgataagcac
      481 tttgcgaccg tgttcgtcac gcaactccgg cgcgtagtaa atattgttca gccactgaat
      541 ttcgcagcgt cttaccggtc gttccaccat tgggcggaac atgtcgcgca gctcaagatc
      601 actcagccac tcaatttcgg tgtcttcttc tgccaggcgt ttttttctga actctgtcgg
      661 gctgtaatgc cgtccgttcg gtttcatcgg cagctcagaa tgcgggcggt tgttgtacca
      721 ttccacgcct tcacgaatag cctcaataag ctccgcccac gatggcagct cgcgcattgc
      781 cgctttctgg cggtcgttca gaggcttgtc ctgttgcagg gcgttaaacg ctgaacgcag
      841 atcacggtta agtacacgca acgattcccg gtcagcaccc ttgccaaaat aggtgcgata
      901 cctcctggct atacgcatcg gcagtgtccg gttcaggcgt tcaataatgc cgcgtccctg
      961 tggattaccg gcaataccgg tagggtgttt aatccccagg cgtggcagaa tacccacgac
     1021 ctctttgtcc agcatgtcag ccgtttcacc ggaaccgtta tccgagtaat acaggaacgg
     1081 ttttccgtgg ttgcgaatgc cgtgctgtat cgcaccggca acggcgaaca cgttttcggc
     1141 aaggtcaaga ctccagccaa caacaaaccg tgttccacca tcgatcacaa acgtgacttc
     1201 cggtgagaat ggacgcccgt ggatcggatg cgcacatttc agcttcatac cgtgaccgtc
     1261 accaatccag acataattca ccggcatagc tgtccagtcg cggcgggtga atccttcaag
     1321 ctgacggtat tcactgccgg taacgcgccc tttttgtttc accacttccg gcagtttttt
     1381 catggcatac cggacaacat cataggaagg catcacctcc agcatgtacg gttcgtccgc
     1441 atggcgacgc tgccattctg cgacaaaatc ctcgtaggct tctgacatgg gtctgccgtt
     1501 cacctggcga tactgcgcca gaaattcagg cagccagctg atttcttccg gttttatttc
     1561 gtcgcgtttt cccggtgcca gtaacagcag ccgttcggcg gcgttctgcg actggttgta
     1621 tttcagaacc cactggtaaa gtggatcgcg gctgatagta cgattagcgc cacgttttgc
     1681 attggcgtta actgcggcct gttgcaggcg ctccggcagc tcacctttac gggctttttc
     1741 aacaagaaag ttaatggctt tcagtcgccc gaggttaccg gcgctttcca gcttcagcac
     1801 ttccaccacc agcgcagctc ttgcttcaga tacccagcgc tggttttcgg tcagcgcatt
     1861 caggcgttct tccatcagtt gtggagaacc gcgatatgct tcgattgcct ggacagcgtc
     1921 aggattgcgt gtttttctgg taacagcttt cacttcagaa acattgttct ctgtagccac
     1981 cagctgttcc acatagcgtt cacgcagtgc ctttcgtgtt atttcaggca gacaatcaat
     2041 gtggtactca atggcttttg tgccttcccg acggcgacta acgtcaccaa gatcacgaga
     2101 aaaacgctgt aacgcctgtc ggatgccttt tgtggtggct ggcattcctg gtacgccgag
     2161 caggtcattt acggtaacgt acgactgcat atcacgacgc cttcctgata aatccatcgt
     2221 tacgataccg gctgggccag atgatttctg gcggtacacc tatagcgtca gaaattacgc
     2281 gttcgtattt tttgtttggt gtgcgaacta ccgctttcag tgaatcctga gcataaccag
     2341 catggagtga aagctcccgg aaggaagacc acccgttatc tttgagcgcc agctgaatct
     2401 tcagcgaagg ccaatcgcgg tcactatctt ctctttgtct catctttcct ttaacctaca
     2461 aaataacccg cgcggatatc cgtatggata aagattaaga taccaaagat tccctataaa
     2521 gggttctttg gtgcttttat ggtttatttt ttaatttcat tatttatcaa tgaattagat
     2581 agaaaggaac caagaatgag aaaagcaccc caaggttctt ttcctgccga tggaaaagaa
     2641 ccgattatcg aaagaatctt taggttagta gaacgatacc cttcaagaaa tgcggctgct
     2701 cgggcatggg ggatcaatga aggcacttta aaaaattatt acaacaggcg ggattacgca
     2761 ccgataccac gcccgcatca gttaaaaaag atagcagaaa gcgaaaatgt taccttggaa
     2821 tggctcatga cgggagaggg tgatgttgaa ctaaaagatt ccaaagaacc caaaaaagag
     2881 aatgcggata atgttgatgc tcaaattcta atgttcttgt catttcttaa accacaagaa
     2941 aaacaacaag tagctgatgt tcttgggaga aaaggagcgg agcaattact cattcttctg
     3001 gatgagcaca tccaagagtt gcacgctcta gttggagttc gtagagccat agcgttaagc
     3061 cttggtaatt tgccagacga aaaggttaga gagatttacg cgttgtccga agctaagggc
     3121 gatcacttaa atctacctca gaaagcagcc agcgcataac ccagtgcaag tgcctagctg
     3181 tgctaaaatt acattggttt tggcgcttgc acccatcagc gctataataa acacctgaat
     3241 cgtttttgaa cggtctttaa aggctattgt aagaacctgt gagttatctt gaatgtttta
     3301 ttgatatcga accgtttaac atttttggac tcgctttatc ttgaaaacct tgagttccta
     3361 acttgttgat tccaatctat tcccgtcttt tttaatctct tcccggttat cttgaataaa
     3421 ttacctagtc atacgagata gaaaccgcca tcgccatcat tcaggcgaaa cagcggcaga
     3481 atatcgaaca ggttgatctc ttcgccatcg acggtgttct gcgcccaggc tggattggcg
     3541 cggcgctccg gggcaatcgg gaagttatcc cagcggcgga taaactcatc aatctgcttt
     3601 ttgaccgggg tgtttggtgg caggccaagg gaaatcgcgt ggttctgcca ggaaccgatg
     3661 gtattcatcg ccacgcgggc atcggtaaag ccgcgaatat tatcaaacca cagcgcgggc
     3721 gcgccgtcgc cgatacgccc ggtggcgtta gctgctgctg ccagatccgg ctcagcgttc
     3781 acttcttcgc tgattttcag taactggccg tggtcatcaa gcgcctgtaa aaagctgcgt
     3841 aaatcatcaa atgccattat tcattctcct gagaaaaatt ccgggcctgc ggcaatcctt
     3901 gccagcgcct ggcgtgggga tgttcgaggc caaattgatc cagcacgcgg gctaccacat
     3961 ggtggacaat gtcatctacc gtttcgggat ggttataaaa ggcaggcatc ggcggcacca
     4021 tcgccacgcc catgcgtgaa agtgcgagca tattttcgag atggatggtg ctaagcggca
     4081 tttcacgcgg caccagcacc agtttgcggc cttctttgag cacgacgtcc gccgcgcgcc
     4141 ctaccaggcc atcggcgtaa ccagcgcgga taccggcgag cgttttcata ctgcacggaa
     4201 taacgatcat gccgtcagta cgaaaagaac cggatgagat gatcgccgcc tgatccgccg
     4261 ggttatggct gaagtctgcg agggcagcaa catcgcgagc gctgtaaggc gtttccagtt
     4321 caatgatggt tttcgcccac ttcgacatca ccagatgagt ctcgacattc ggcatctcac
     4381 gcagcgcttg cagtaatgcc acaccaagag gcgcaccggt agcccctgtc atcccgacga
     4441 tcagtttcat tgcttattct cttaattgtt cgtatacgaa cgttattatt aacatactcc
     4501 ttcatttcca cagcggcaag gattgaaaag taacaacatc ttcttcgtca ctaaaaaacc
     4561 gctatgatag cgacagagaa ttagcccgga gtttacatgg cgttacgaaa taaagcgttt
     4621 catcagttac gacagctttt tcagcagcac acggctcgct ggcagcacga gttacctgac
     4681 ctgaccaaac cacagtacgc ggtgatgcgc gccattgctg ataaacctgg tattgaacag
     4741 gtggcactga tagaagcagc agtcagcacc aaagcaacgc tagcagaaat gttggcaaga
     4801 atggaaaatc gtggcttagt cagacgtgaa catgacgtcg ctgacaagcg gcgacgcttt
     4861 gtctggctga ctgccgaagg agaaaagcta cttgctgcgg cgatacctat tggtgacagc
     4921 gtggatgagg aatttttggg gcgtttgagt ggagaagagc aggagctgtt tatgcagttg
     4981 gtgcgcaaga tgatgagcac atagaatgct gattgccggg taggacgctg acgtgtctta
     5041 tccaggcgac aaaaacacaa gcttcccaaa caaaagaaaa aggccagcct cgcttgagac
     5101 tggcctttct gacagatgct tacttactcg cggaacagcg cttcgatatt cagcccctgc
     5161 gtttgcagga tttcgcgcaa acggcgcagg ccttcaacct gaatctggcg aacacgttca
     5221 cgggtgaggc caatttcacg acctacatct tccagtgttg ccgcttcgta ccccagcaaa
     5281 ccgaatcgac gtgccagtac ttcacgctgt ttggcgttca gctcgaacag ccatttgacg
     5341 atgctctgct tcatatcgtc atcttgcgtg gtatcttccg gaccgttctc tttttcatcg
     5401 gccaggatgt ccagcaacgc tttttcggaa tcaccaccca gcggggtgtc taccgaggta
     5461 atgcgctcgt taagacgaag catacggctg acgtcatcaa ctggcttatc cagttgctct
     5521 gcgatctctt ccgcacttgg ttcatggtcc agcttatggg acaactcacg tgcggttcgc
     5581 aggtaaacgt tcagctcctt tacgatgtga atcggcaaac gaatagtacg ggtttggttc
     5641 ataatcgccc gttcaatcgt ctggcgaatc caccaggttg cgtatgttga gaagcggaaa
     5701 ccacgttccg ggtcaaactt ctctaccgcg cggatcagcc ccaagttgcc ctcttcgata
     5761 aggtccagca acgccagacc acgattgcca taacggcggg caatttttac caccagacgc
     5821 aagttactct cgatcatccg gcggcgagag gcgacatctc cacgcagtgc gcgacgcgca
     5881 aaataaactt cttcttcggc cgttaacagt ggtgaataac caatctcacc aaggtaaagc
     5941 tgagtcgcgt ccaacacacg ctgtgtggct ccctgcgata acagttcctc ttcggccaaa
     6001 tcgttatcac tgggttcctc ttctactaag gccttttcgt caaaaacctc aactccgttc
     6061 tcatcaaatt ccgcatcttc atttaaatca tgaactttca gcgtattctg actcataagg
     6121 tggctcctac ccgtgatccc ttgacggaac attcaagcaa aagcctggtt ccgccgattt
     6181 atcgctgcgg caaataacgc agcgggttta cggatttccc cttgtaacga atttcaaaat
     6241 gcaagcgtgt tgaactggtt ccggtgctac ccatggttgc tattttttgc cccgccttca
     6301 cttcttgttg ttcccggacc agcattgtgt cgttatgggc gtaggcactc aggtaatcat
     6361 cattatgttt gatgataatc agattaccgt agccgcgcag cgcgttacca gcataaacaa
     6421 cgcggccatc tgcggtcgcg ataattgcct gtcctttgct gcctgcgata tcaatcccct
     6481 tgttgccccc ctcagaagcg ccaaaggttt cgatcacttt gccctcagtc ggccagcgcc
     6541 aggtggagat aggcgtactg gttgatgtac tgctgacagt cggctcggtt gtgcttgctg
     6601 ttggtaccgt tacaggcgct gtgaccgtgg tcgcagttgg cttgttgttc ggcaacattt
     6661 tgttagcact cagttcaccc gaagactcag aatacgtaat tgtcggttgc gacgcaacag
     6721 caacggtgga attttgtgca ggcttgatca caactccttg ctctgctgcg tcggcctggg
     6781 taatagcatt tccgccagtg attggcgtac cggaagcatt acccacctgc aaggtctgac
     6841 caacgttcag cgcgtatggt gcctgaatat tgttgcgctg agcaaggtca cggaaatcgt
     6901 tgccagtaat ccaggcgata tagaaaagtg tgtcaccttt tttcacggta taggtactgc
     6961 cgctataact gcctttcgga atgttcccat actgacggtt atagacgatg cgtccgtttt
     7021 ccatctgtac cggctgctga gctactggct gcactggctg gatttgcggt tgttgagtag
     7081 cctgaatttg tggctgctgt accggctgaa tttgcggttg ctgcgctgta gacgtcgtcc
     7141 ctattttcgg cggcggcgta atcaacatac cagaattagt atttgcaggc gcattgccat
     7201 taacggagct gaccggtgcc ggtggatttg aagtgtcaga acagcctgcc agccatagcg
     7261 aaaccagtga caaagccgca atgcggcgaa cggtgaattt tgggcttccc gcgctcattt
     7321 atcccccagg aaaaattggt taataaccag tgacataatt accgtgcaag gcaccctact
     7381 gaacactgga aaagatgttc acgatacgct gacctacggc aaaataacca ggaaaaatcc
     7441 aggtatttcc tcacgtttta agccagctca cccttcacta aagggacaaa gcgcacggcc
     7501 tccacggtat cgataataaa ttcgcctccc cgacgacgca cccgtttcaa atactggtgc
     7561 tcctccccta cgggtaagac gagaatcccg ccttcgtcca gctgcgtcat tagcgcagtt
     7621 ggaatttccg gcggtgccgc cgtaacaatg atagcgtcaa acggcgcacg tgcctgccaa
     7681 ccttgccatc catcaccatg acgggttgaa acattatgta aatcaagatt tttcaggcgg
     7741 cgacgtgcct gccactgcaa gcctttaatc cgttcaaccg agcaaacatg ctggacaaga
     7801 tgcgccagga ttgccgtttg atatcccgaa ccagtgccaa tttccagcac ccgcgactgc
     7861 ggcgtcagct cgagtaattc ggtcattcgc gccaccatat acggctgcga aattgtctgc
     7921 ccctgaccta tcggcaaggc gatattgtcc caggcttttt gttcaaacgc ttcatcaacg
     7981 aatttttcac gcggcacggc ggcaagtgca ttcagcacct gctcatcctg aataccttgc
     8041 gcacgtaatt gatccagaag tgcttgtacg cgtctgctta ccattgcgtg ccaactccca
     8101 cgctgtttaa ccagtctgaa accacatctt gcgcgctatg cgcagttaaa tccacatgca
     8161 gcggcgtgat ggagacatag ccctcatcta ccgcagcaaa atcggtcccc ggaccggcat
     8221 cacatttacc gcccggcggg ccaatccagt acagcgtatt gccgcgcgga tcttgctgcg
     8281 ggatcacctg atctgccgga tgtcgtgtac cgcagcgcgt cacgcgaata cctttgattt
     8341 gatccaaggg taaatccgga acgttaatat taagaatacg tccggtgcgc agcggctctt
     8401 tacacagtgc gcgcaaaatt gaacaggtta ccgccgcggc agtgtcgtaa tgtttatgcc
     8461 cgtcaagcga gacggccagc gccggaaaac ctaaatgacg gccttccatc gcggcggcta
     8521 ccgtaccgga ataaataaca tcatccccca gattcggccc ggcgttaatt ccggacacaa
     8581 caatgtccgg gcgcggacgc atcagagcat tcacgccaag atagacacaa tcggtcggag
     8641 ttcccatttg cacagcaata tcaccatttt caaaggtaaa cgtgcgcagg gaggattcca
     8701 gtgtcagaga atttgaagcg ctgctgcggt tacgatcggg ggcgaccacc tgaacgtcag
     8761 caaactcacg caaggctttc gccagcgttt gtataccggg tgcatgtacc ccgtcatcat
     8821 tactcagcaa tatgcgcata atcacctgtt gtgttgataa gttcccttac aacgctggtt
     8881 gcaaaactac ccgccggaag ccagaaacgg atctctacgg tgacgtcatc ccaccagttc
     8941 cagcttaatt gttgcggata cagcagcatc gctctgcgcg cggcttcaac tttttcgcgc
     9001 accagtaaag cttgtaattc agtttctgcg gcgacagctg cttgttcgaa tgccagcgct
     9061 tcacgctgag ttccccattc gccactgccc ggcaatgcgg cggttatcat cagctcttta
     9121 tcgttgacgc gacgctgtaa ttccgccagt tcttcggtgg ttgctacaaa ccagctacca
     9181 cgtccggcta attgtagcgc atcgccgtca acaacttgat taacgtctgc ttttttgagg
     9241 cgctcagcaa caatctgatt aaacaacgca ctgcgggctg ccgacaacca aaaactccgt
     9301 ttattgcgat cgcgcaccgg agtattggtt tgcgcccagc gcagcgcgcc ctgcaagttg
     9361 ctaccgccaa tcccaaaacg ttgggcaccg aagtagttcg gtacaccttt tacgcaaata
     9421 tcgatcagac gttgttcaac gtcatcgcga ttgctcactt cgcgcagaac cagggtaaag
     9481 gcgttacctt tcagcgcccc taaacgcagc ttgcgcttgt gccgcgcata ctccagcacc
     9541 tggcagcctt ccagttgaaa ggcgctcaga tcgggcattt ccttgcccgg cacgcgagca
     9601 cataaccact gttccgttac agcatgtttg tctttttgcc cagcgaagct gacttcacgg
     9661 gaatgaattt tcaggaattt cgccagtgca tccgccacaa aacgggtatt gcagccgttt
     9721 ttgaggattc taaccagaat atgctcacct tcaccatcag gctcaaagcc caaatcttcc
     9781 accaccacaa agtcttccgg attggctttc agcagcccgg tgccttgcgg tttaccgtgg
     9841 aggtaagtga gattatcaaa ctcaatcatt ttgttgcctt aatgagtagc gccaccgctt
     9901 cacaggcaat cccttcccca cgtccggtaa atccaagttt ttccgtagta gtggctttca
     9961 cgttaacatc atccatatgg cagccgagat cttcggcaat aaacacgcgc atttgtggaa
    10021 tgtgcggcaa catcttcggt gcctgagcga tgatagtgac atcgacgttg ccaagggtat
    10081 aacccttcgc ctgaatacga cgccaggctt cgcgtagcag ctcgcggctg tcggcacctt
    10141 taaatgccgg atcggtatcc gggaacagtt tgccgatatc ccccagcgcc gccgcgccaa
    10201 gcaatgcatc ggtcaacgca tgaagcgcca cgtcgccatc agaatgcgcc agcaatcctt
    10261 tttcgtaagg aatgcgtacg ccaccaatga taattgggcc ttcaccgcca aaggcgtgta
    10321 cgtcaaaacc gtgtccaatt cgcattatgt attctcctga tggatggttc gggtgaggta
    10381 aaactcggcc agtgccaaat cttccgggcg cgtgacttta atgttatccg cgcggccttc
    10441 gaccaactgc ggatgaaatc cgcaatattc cagcgccgag gcttcgtcgg taatggtagc
    10501 gccttcattt agagcgcgcg tcagacagtc atgtaacagc tcacgaggga aaaattgcgg
    10561 cgtcagcgcg tgccataagc cgttgcgatc aacggtatga gcaatggcat ttttgcccgg
    10621 ttcggcacgt ttcatagtat cgcgcactgg tgcggcgagg atccctcccg tgcggctggt
    10681 ttcgctcaac gccaacaatc gcgcgaggtc atcctgatgc aaacaaggac gagcggcgtc
    10741 atgcaccaat acccactgca catcgccagc ggctttcaga cctgccagca cggaatcggc
    10801 acgctcatca ccgccatcta caacggtgat ttgcggatga ttcgccagag gaagttgtgc
    10861 aaaacggcta tcgccaggac ttatggcaat gacgacacgt gtcacccggg gatgcgccag
    10921 cagcgcatgc accgagtgtt caagaatggt ttgattaccg attgagagat attgcttagg
    10981 acattccgtt tgcattcgac ggccaaatcc ggccgccgga accacggcgc aaacatccaa
    11041 atgagtggtt gccatgttaa ttcccgggct gatttatcga ttgttttgcc ccgcagactg
    11101 tgcgcgcttc gacgcgtcag gcaccagacg ataaaaagtt tcgcccggcc tggtcatgct
    11161 gagttcatta cgcgcacgct cttcgagcgc ctcctggccg ccattgagat cgtcaatttc
    11221 ggcaaaaagt tgatcgtttc gcgctttaag tttcgcgttt gtagcttgca gtgccgccac
    11281 atcatcattg acgcgggtat agtcatgtat accgttctta ccgaaccaca gcgaatactg
    11341 tagccagacc agaatagcca gcaacagcag cgttagttta cccatcctgc cccctgaaaa
    11401 actgcatcat catcccatgc atccgaagac gactctacat cctctgttgg ggataccgcg
    11461 acaacgcggg caaatgtacc acatttgtcc attgttacgt atacccaggg cgtgcagaac
    11521 ataatctcat tattagttac ggtttgaatt atgaacagag gagacaagaa agtacaaatt
    11581 agcccagtag ccacataaac agtgcgccaa acataatgcc tactgtcacc agggtgaaaa
    11641 caatactgta gcgtagcttt ccgtccatca atgaatgcag cgcaatcccc accactaccg
    11701 cgacaggcat cagtgccaga aagaaaggcc aggtgtagat aaagaagaac agcgtgttag
    11761 agccataaat caacatcggc atcgccagcg caaataacca ggagataaaa ccgaccacgg
    11821 caccaggcag tgaccatgta gtttcttcat cctcagtaag gctgtcgtta tttgttagtg
    11881 taatgttatg gctattacgc atatttgatc ctgttacttt gacgaaccgg gcatggaaac
    11941 ccggtggtgt ctcaggatct gataatatcg ttctgtctca acagatctaa taattgctgt
    12001 accaaatttg ttactaattg ttcaccattg agatgaattt ctgccgattc aggctcttcg
    12061 taaacggaat ctattcccgt aaagttgcgc agttcaccgg cacgcgcttt cttatataag
    12121 ccttttggat cgcgggcttc gcaaatcgcc agcggcgtat cgacaaacac ttcaataaag
    12181 cgcccttctc ctacgcgttc gcgaaccatc tggcgttcgg cgcggtgtgg cgagataaat
    12241 gcggtcagca ccaccagtcc ggctgcaacc atcaaattcg ccacttcacc gacgcgacgg
    12301 atattctctt tacgatcggc atcgctaaaa ccgagatcgc tgcataatcc gtggcgaaca
    12361 ttgtcgccat ccagcagata cgtactgacg ccgagtttat gtaacgcctc ctccagcgcc
    12421 ccggcgaccg ttgatttacc ggacccggag aggccggtaa accacagcac tacaccacga
    12481 tgaccgtggt gtagttcgcg ttgttgcaca gtgaccggat gactatgcca gacgacgttt
    12541 tcgtcatgca gcgccattat ttatccccca gcaaatcgcg cgcgccccag tgcggaaagt
    12601 ggcgacgaac cagggcattc aattccagtt cgaatgcact gaattcagat gacgcagctg
    12661 ttgcctggct aactggctcg tgcaccatac cggcacccac ggtcacattg ctcaggcgat
    12721 cgataaaaat cagcccaccg gtaaccggat tttgctgata gcgatctaac accagtggct
    12781 cgtcaaaagt gagatccacc aggccgatgc cgttcagcgg caggttttca acttcgcgtt
    12841 gggtaaggtt attaatatca acctgataac gaatgccatc aacacgagca cgcgtcttct
    12901 taccggcaat tttgatgtca taactctggc ccggggaaag cggctgttcc gccatccata
    12961 ccacatccac cgacgcactc tgcaccgccg gtaacgcttc gtctgccgcc agcagcagat
    13021 caccacggct gatatcgatc tcatccgtca gcaccagggt gatcgcttct ccggcaaaga
    13081 cttcttcgcg atcaccatca aaagtcacga tccgcgcgac gtttgattcc acaccagagg
    13141 gcagcacttt tacacgttgc ccgacttcca cgcgaccgga tgccagcatt ccggcgtagc
    13201 cacgaaaatc gagatttggg cggttaacgt actgcaccgg gaagcgcatt ggctgggcat
    13261 ccaccactcg ctggatctcc acggtttcca gcacttcgag cagtgtcaga ccgctgtacc
    13321 acggcatact ttcactttgc gaagccacgt tgtcaccttc cagtgcggag agcggcacaa
    13381 agcggatatc cagattaccc ggcagctgcc cggcaaaggt cagataatct tcacgaatac
    13441 gggtgaacgt ctcttcactg taatccacca gatccatttt gttgatcgcc acgaccagat
    13501 gtttgatccc caacagtgtg gagataaaac tgtgacgacg ggtttgatcg agcacgcctt
    13561 tacgggcatc aatcagtaag atcgccagtt cacatgtcga tgcgccagtc gccatattgc
    13621 gagtgtactg ctcgtgccct ggggtgtcgg cgataataaa tttacgcttc tcggtagaga
    13681 aatagcggta ggccacatca atagtgatgc cctgttcgcg ctcagcttgc aggccgtcca
    13741 ccagcagagc cagatccagc ttttcgccct gggtgccgtg acgcttactg tcgttatgca
    13801 gcgatgagag ctgatcttcg tagatttggc gggtatcgtg cagcagacga ccaatcaggg
    13861 tacttttgcc gtcatcgacg ctaccacagg tcagaaaacg cagcaggctt ttatgttgtt
    13921 gcgcaatcat ccaggcttcg acgccgcctt cattggcgat ttgttgtgca agtacggtgt
    13981 tcatcttaaa aatacccctg acgttttttc agctccatgg atcccgcctg gtcgcggtca
    14041 atcacgcgcc cctgacgttc actggtggtg gaaaccagca tctcttcgat gatttccggc
    14101 agtgtttgtg catttgactc caccgcaccg gtcagcggcc agcagcccag cgtacggaaa
    14161 cgcaccatcc gttttttgat cacttcgccc ggttgcaggt cgatacggtt gtcatcaatc
    14221 atcatcaaca taccgtcgcg ttccagaacc ggacgttccg cagcgagata cagcggcaca
    14281 atgtcgatat tttccagcca gatgtattgc cagatatcct gctcggtcca gttagagagc
    14341 gggaagacgc ggatgctttc gcctttgtta atttgcccgt tgtagttgtg ccacagctcc
    14401 gggcgctgat ttttcggatc ccagcgatgg aagcggtcac ggaaagagta gattcgctct
    14461 ttagcgcggg atttctcttc gtcacggcgc gcaccaccga aggcggcatc aaaaccgtat
    14521 ttgttcagcg cctgtttcag gccttcagtt ttcataatat cggtatgttt cgcgctgccg
    14581 tgcacgaatg gattaatccc catcgccacg ccttccgggt ttttatgcac cagcagctcg
    14641 cagccgtagg ctttcgccgt acgatcgcgg aactcataca tctcgcggaa tttccagccg
    14701 gtatcgacat gcagcaacgg gaaaggcagc gtacctggat aaaacgcctt gcgcgccaga
    14761 tgcagcatga cgctggaatc tttaccgata gagtagagca tcaccggatt tgagaattct
    14821 gccgccacct cgcgaataat gtggatgctt tccgcctcca gttgccgcag gtgagtaagt
    14881 cgtttttgat ccataaccgt tcctttgcaa taccgctatt ttcttgccat cagatgtttc
    14941 gactataggg agcgtaagag aacgaatgaa attaccaatt agaatgagta gttccttaac
    15001 ggaataacga tttggcaaag ctaatatcaa aaagtgctta aggcaccgga tttcgggcgt
    15061 ttaggaagat ttgaaattgt tttagcgcag cggcagtttc atactatggc ggtaaaaaaa
    15121 tttgcatggt atttaaggac tcactatgtt ttccgcattg cgccaccgta ccgctgccct
    15181 ggcgctcggc gtatgcttta ttctccccgt acacgcctcg tcacctaaac ctggcgattt
    15241 tgctaatacc caggcacgac atattgctac tttctttccg ggacgcatga ccggaactcc
    15301 agcagaaatg ttatctgccg attatattcg ccaacagttt cagcaaatgg gttatcgcag
    15361 tgatattcgg acatttaata gtcggtatat ttataccgcc cgcgataatc gcaagaactg
    15421 gcataacgtg acaggaagta cggtgattgc cgctcatgaa ggcaaagcgc cgcagcagat
    15481 catcattatg gcgcatctgg atacttacgc cccgctgagc gatgctgacg cagatgccaa
    15541 tctcggcgga ctgacgttac aaggaataga tgataacgcc gcaggtttag gtgtcatgct
    15601 ggaactggca gaacgcctga aaaatacgcc taccgagtat ggtattcgat ttgtggcgac
    15661 cagcggcgaa gaggaaggaa aattaggcgc tgagaattta ctcaagcgga tgagtgacac
    15721 cgaaaagaaa aatacgctgc tggtgattaa tctcgataac ttaattgttg gcgataaatt
    15781 gtatttcaac agcggtgtaa aaacccctga agcagtaagg aaattaacgc gcgacagggc
    15841 gctggcaatt gcacgcagtc acggaatagc cgcaacgacc aatccgggtt tgaataaaaa
    15901 ttatccgaaa ggcactgggt gttgtaatga cgcagaaata ttcgacaaag cgggcattgc
    15961 tgtactttcg gtggaagcga ctaactggaa tcttggaaat aaggatggtt atcagcaacg
    16021 cgcaaaaaca cctgccttcc cggcgggaaa tagctggcat gacgtaagac tggataatca
    16081 acaacatatt gataaggcac ttccaggaag aatagaacgt cgctgccgtg acgttatgcg
    16141 gataatgcta ccgttggtga aggagctggc gaaggcgtct tgatgggttt gaaaatggga
    16201 gctgggagtt ctaccgcagg ggcggggaat tctaagtgat acccatcatc gcatccagtg
    16261 cgtccggttt acttccggga aaaccgcacc gtattgcgcc ggtttatctc cgctggagcg
    16321 gggaacacct ctggcgaata gaaagcaagc caggttttga ccagtcgatc gtacatggca
    16381 ttggccgttg ccattgttgg caggccaaaa aacagcccct gagcgttacc cgcagccatc
    16441 aacctgtgcg ccaggataag cgccgcttct gttttacctg cgccggtcac atcttccaga
    16501 ataaacaact gtggtcctgg ctgattgata tccagatcca gcaccttttg ctgtaacggt
    16561 gtcgtattct caataaaagg aaacagcgta ttaattccgg tgaaaggcgc ggtttctgct
    16621 tgtggcggaa agacggttaa tgcgttttga gcctgaacta aagccttttg ccagtaatct
    16681 tcaatatcca ttgcctgtgc gacgcgtgga aaaaatcgcg ttgacgaccc tgtccagtct
    16741 gcaagtacga cggttgcaga gatataccag gaaagttgtt ttataagttc aacgccctcg
    16801 tcatcatccc agaatgtggg gatctctatt cgcggaaaca gtggcttgat ttcaaggaga
    16861 aaatctcgtg ctgcagcttt gtcttcaggc agaaaattat ccaattcatc catacgatca
    16921 gggggccgac catgatgccc tgtcgtcacg ggcatccaca tttctatcac tcgcctaagt
    16981 ttgcgcggag agagcgaaga tgaaggaagt aactcctgac attcacttaa ataatgattc
    17041 cacaaccagt agcccagcgt tgaatgagag attttttcgt aattttttct ggcaccttcc
    17101 tgaatcttga attcaggggg caggtaaagt tgttgaaaag agcgggcaaa ttttccaata
    17161 tcgtgccagc acagcaacca ggcgaaaaat tgagctgcct gttccttgtc agaaatccct
    17221 aattgacgaa aataatcagc cagcccgaag caatttcttt taaccattaa atagcccatt
    17281 gcggccacat ccagcaaatg ccagcaaaga aggtgatagt cgtcgctacc ctctttctcg
    17341 ccacgtcggt tttttcccca gaaatcaaag aaagtcacaa tatttttatc cttcagtaaa
    17401 cttaaaggat atttacgcat attttaaaaa ctatctgtga tatatatcag ccaataaaac
    17461 atttacatta taaataaatt aatgattttc atttgaaatt cataataata aatagagact
    17521 atatattcac ataaaaaata taaattttaa aaaagggtgc aggagaccaa accatcacaa
    17581 ttcagtacgc ggggttttac ccccgcgtgg tggctacttc ttcgattcgt aagcgagaac
    17641 tgccttaaac tccataccac gttctctgat actcaactca cataacgagt cacctaccat
    17701 caacgtgaat cccagtactg tacaacacag tacgatgatt gccacaaggg catattttgt
    17761 cagcatgtct tgcctccctg caaagaagag actaacatcc gaattgctac gttcgaacgc
    17821 gagcctcagg ttgatagaaa tatcgcctgg ggcttttgtc catctggaac ctcgcgaatg
    17881 cttaacgcca gacagcctca agcacccaac gccattctat acctgttaat tttccctgcg
    17941 atgttcacct ttaaatgccg gaatgctgct cgcaaaataa aaacgccccc atcatttctg
    18001 acagaggcgt ttaatttatc cggcaatatt tacccttcgt gtaatccaca ttcccttttt
    18061 aagccaaaga aacgcgtttc ttcttccgcc atgccgggtt cccatttacg ggttgtatgg
    18121 gtatcgccca ccgataaata tccttcgtcc cataatgggt gatatttcag gccatgtttt
    18181 tgcaggtact gataaatagt tcggttatcc cagtcgataa tcggcagcac tttaaatacg
    18241 ccacgctgaa ttgccagcac cggtaaattg gcacggctgc cggattgttc acgccgcagg
    18301 ccagcaaacc acgtttgcgc attcagttct ttcagcgccc ggttcatcgg ttcgactttg
    18361 ttgatgtcat tgtacttttc aatgccttca acgccctgtt cccacagttt gccgtagcgt
    18421 gcttcctgcc aggcggcgct ttcggtagcg cggtacactt tcaggttgag cttgagtttg
    18481 tccgttaact cgtcaataaa gcggtaggtt tccgggaaca gatagccggt gtcggtgagg
    18541 atcaccggaa tatccgggtg aatctgattc accagatgca ggctcaccgc cgcctgaatg
    18601 ccaaagctgg aagaaagtac atattcaccg ggcagattat ccagcgccca ggcaacgcgg
    18661 ccttcagcgt ccagtttttc cagttcggcg ttagtttctg ccagcgccag aatgcgatct
    18721 accttcggca gttcgttcag ggcgtttaga tcgagtttgg acatagattc ctcactgttt
    18781 gccttgcctg atgcgacgct cacgcgtctt atcaggccta caagaccggg ctgatggtta
    18841 atcccacaaa tcgcgcgccg gatcgagcac cgggcgaatg atgcccgcac gcaccgtaaa
    18901 gtcgccgaag ccttcacccg cttcgcgctc tttcgcccag cgccctatca gttcatcaag
    18961 cgacgccagg atttccggct cggtgatgtt ttctttatac atccgtggga tacgtgtccc
    19021 aatgcggttg ccgccaagat gcaggttgta gcgacccggt gctttaccca ccaggcccac
    19081 ttccgccagc atcgcgcgac cacaaccgtt cgggcagcct gttacacgca tcacgatatg
    19141 ctcatcgctg acaccatgtt tcgccattaa attatcgatg ttgtcgataa aagacggcag
    19201 gaaacgctcc gcttccgcca tcgccagcgg gcaagtcggg aatgacacgc aggccatcga
    19261 gttttcacgc tgcggcgtga cggcattcat taacccgctc tctttggcaa ttttctcgat
    19321 cttcgctttc tcgctttccg gcacaccggc gatgatcaga ttctggttcg ccgtaatgcg
    19381 gaaatcacct ttgtggatct tcgcgatctc cagcaggcca gttttcagcg gacgccccgg
    19441 ataatcaagg atgcgaccat tttcgataaa cagcgtcagg tgccagttat catcaatgcc
    19501 cttaacccag ccaatacgat cgccgcgtcc tgtaaattca tacgggcgga tcggttcaaa
    19561 tttgatcccc gcgcgacgct ccacttccgc tttaaacgtc tcaaccccca cgcgctccag
    19621 tgtgtatttt gttttggcat ttttacgatc ggttcggtta ccccagtcac gctgagttgt
    19681 cacgacggct tccgccaccg ctagcgtatg ctctagcggc agatagccaa actcactcgc
    19741 cgtgcgggcg taggttttct tgttaccgtg ttcgatagaa agcccaccgc ccaccagcag
    19801 gttgaagccc accagcttgc cgttttccgc gatcgccacg aagttcatgt cgttggcgtg
    19861 cagatcaata tcgttctgcg gcgggatcac taccgtggtt ttgaacttac gcggcaggta
    19921 ggtctgacca aggatcggtt cttcatcggt ggtggcgact ttttcctgat cgagccagat
    19981 ctccgcatac gcgcgggtac gcggcagcag atgttcagaa atcttcttcg cccactcgta
    20041 cgcttctgcg tgcagctgcg actcgtaagg gttcgaggtg cagagtacgt tacggttcat
    20101 gtcgttagca gtcgccagcg catcaagacc gaccgagtgc agcatctggt gcaccggttt
    20161 gacgttcttt ttcagaatgc cgtggaactg aaacgtctgg cggttggtca ggcgaatgct
    20221 gccatagatg gtgttttcac cggcaaattt atcgatcgcc tgccactgtt tggtggtgat
    20281 aactccaccc ggcagacggc agcgcaacag catcgcgtgg cgcggctcca gcttctgttc
    20341 agcacgttcg gcgcggatgt cgcggtcatc ctgctgatac atgccgtgga agcggatcag
    20401 caggaagtta tcgcccttaa agccgccggt cagaccgtcg tttaaatctt ccgcaatggt
    20461 gccgcgcagg tagttgcttt caagcttcat gcgctcggcg tctgtcagtt ttccttcgac
    20521 cactaaaggc cctggatgtt tttcgctcat tagtagacat ctcgctgata acggcgctct
    20581 acgcgcagct cacttaaaaa ttcatccgcc gcttcggtgt ccatgccacc aaattcggca
    20641 atcacttcca gaagtgcctg ctcaacgtct ttcgccatgc gattagcgtc gccgcagaca
    20701 taaatgtggg caccgtcatt gatccagcgc cacagctccg cgccctgttc gcgcagttta
    20761 tcttgtacgt aaactttttc tttttggtcg cgcgaccagg cgagatcgat ccgcgtcagc
    20821 acaccctctt tgacgtagcg ctgccactcc acctgataca ggaagtcttc cgtaaaatgc
    20881 ggattgccaa agaacagcca gtttttacct ggcgcttcgt cagcggcgcg ttgctgcata
    20941 aaggcgcgga acggcgcaat accggtgcct gggccaatca taatcaccgg ggtttccagg
    21001 ttagcgggca ggcggaagtt atcgttatgt tcgataaata cgcggacttc gccctcttct
    21061 tccacgcggt cagcgaggaa gctggaggca ccaccggcac gggcgcggcc ttccacgtcg
    21121 taacgcacca caccaacggt gacgtgtact tcgttctcga cttccgcctg cgaggaggca
    21181 atggaataga gacgcggggt cagcgggcgc agcagattaa ttagcgcttc ggcatcaagc
    21241 tgtgccgggg agaaacgcac catgtcaaca atcagcgtcg tcgcggcgta atgctgtaac
    21301 ttcgctttat cgcccaccag cggcagcagt gtttcactgc gggtaagcgt ggcgtaattc
    21361 tcaacaatgt tggcggtgtt gacggtcagt tcgaagtgcc actgtagcgc ttcgttcaga
    21421 ggcaacgttt tgccctcgac agtgacaggt tcatcgcctg tcagccacag cagttcgaca
    21481 agttctttca ccagtgccgg atcgttctga taccagacgc ccagcgcgtc acccggctgg
    21541 taacgcaggc ccgagtcgcc taagtcaatt tcgatatggc gaacgtcttt ttcagagtta
    21601 cgcccagtaa ttttctggtt aacagagagg ctagccacca gcggcgcgtc tttgctgtac
    21661 gggctggtgt ggatttcatt taccgcgcca gtagcgacgg attgcgaagg tgccgcgaca
    21721 ggcgcacgcg atttaagcgc atcaaccacg cgggcgcgcc actcgctggc agcagcctgg
    21781 tattcaacat cagaatcgac acggtcgagc aggcgttcac cacccagttc cgccagcttg
    21841 ctgtcaaaat ctttcccgga ctggcagaaa aattcataag agctatcgcc gaggctaaac
    21901 acggcaaacg cggtgttttc cagctttggc gcttttttgg agaacaggaa cttatgcagc
    21961 gcgacggctt cttccggcgg ttccccttcc ccttgcgttg acgtcactac gatgagcagt
    22021 ttttcgctgg cgatttgttt gaatttatag tcgcccgcgt tcaccagctt aacgttcagt
    22081 tttgctgcta ataaatcatc gcgtaatgct tcagcaaccc ggcgcgcatt gccggtttgc
    22141 gaagcagaga taatcgttat acccggcatt tctgcggcgg gcgctggcgt cactgcaagc
    22201 gcaacaggct gctggttgag cacgccccag aaatagccag aaacccaggc aagctgggta
    22261 ggcgttaaat cggtcgtggc cgcctgaagg cgtgccagtt gctccgggtt caacggaagc
    22321 aacgcggaag gtgggacctg tgtcgtcatg cgtcgttatg ttccagtaag caaagctgtt
    22381 tctgcgccct gtcagcgccc ataaaacaga agagaaggta aggttaacgg ggtgaacggt
    22441 gtggattaaa gacgggatag cgataactaa taaccaaatc gactaacctg ttttagcaat
    22501 agtctttaac aacaaaatag attaaccaac ctaatgaaaa acaaatgaat ttagccaatc
    22561 attaagataa atcagcgatt ttgcgcaaca aagtcgtttt agataatgcg aaaaaacagc
    22621 ctttccggta ctctacggcg gttttatcgt cctgtagaga aattatgatg tccaccacgt
    22681 tatttaaaga tttcaccttc gaagccgctc accgcttacc acatgtcccg gaagggcata
    22741 aatgtggtcg cctgcacggg cattccttta tggtgcgact ggaaattacc ggggaagtcg
    22801 atccgcatac gggctggatt atcgatttcg ctgaactgaa agcggcgttt aagccaactt
    22861 atgagcgcct cgatcaccat tatctcaatg atattccagg tctggaaaac ccaaccagcg
    22921 aagttttagc aaaatggatt tgggatcagg ttaaacccgt tgtgccgctg ttaagtgcgg
    22981 tgatggtaaa agaaacctgc accgcagggt gtatctatcg cggcgaatga taagagtgtg
    23041 tcggcggtca atttcccttt agtaacgcta tgttagggtg ttgtgttctg gatatctggg
    23101 gcatgacatg gaagacgact gcgacattat tattattggt gccggtattg caggcaccgc
    23161 ttgcgcgtta cgctgcgcgc gagcggattt atccgttttg ttactcgaac gcgctgaaat
    23221 ccccggcagc aaaaatcttt ccggcaggcg gttatatacc catgcactcg cggaactcct
    23281 cccgcaattt catctgaccg cgcctcttga acgacgcatc actcacgaaa gcctttccct
    23341 gttaacgccg gatggcgcaa cgacgttttc cagcttacag cccggcggtg aatcctggag
    23401 tgtattacgt gcacggttcg atccgtggct ggttgccgaa gccgaaaaag aaggtgtcga
    23461 atgcatcccc ggagcgacgg tggatgcact gtatgaagaa aacggcagag tctgtggcgt
    23521 tatttgtggt gacgatattc tccgcgcccg ttatgtggtg ctggcagaag gtgccaacag
    23581 cgtcctggct gaacgtcacg ggttagtgac tcgtcctgct ggcgaagcga tggcgttggg
    23641 gatcaaagaa gtgctgtcgc tggaaacatc cgctattgaa gaacgttttc atctggagaa
    23701 taacgaaggc gcagcgttgc tgttcagcgg cggaatctgt gatgacttac ccggcggcgc
    23761 atttctttat actaatcaac aaacgctctc gttagggatt gtttgcccgc tctcttccct
    23821 tacgcaaagt cgtgttccgg caagcgagct gctgactcgc tttaaagcgc atccggcagt
    23881 gcgcacgctt atcaaaaaca cggaatcact ggagtatggt gcgcatctgg tgccagaagg
    23941 tggcttgcac agtatgccgg tgcaatacgc cggtaacggc tggctgctgg tgggcgatgc
    24001 gttgcgcagt tgcgtcaata ccggaatttc cgtgcgcggc atggatatgg cgctgactgg
    24061 cgcacaggcg gcggcacaaa cgctaataag cgcctgccag caccgcgagc cgcaaaatct
    24121 gtttgcgctt tatcatcaca acgtagagcg cagcctgcta tgggatgttc tacaacgtta
    24181 tcagcatgtt ccggcgcttt tgcaacgccc tggctggtat cgggcgtggc ctgcgttaat
    24241 gcaggatatt tcccgcgatt tatgggatca gggtgataaa cctgttccac cgctgcgcca
    24301 gttattctgg cgtcatttac gtcgtcatgg cctgtggcat ctggcgggcg atgttatcag
    24361 gagtctgcga tgtctgtagc ccgtaatctc tggcgcgttg ctgatgcgcc gcacattgtt
    24421 ccggctgact ccgttgagcg tcagatggta caacggttga ttaacgcctg tccggtaggt
    24481 cttttttctc tcacacagga aggtggctta cgtattgact atcgcggctg cctggagtgt
    24541 ggcacctgcc gtttgctgtg cgacgaatcc acactacaac agtggcgcta tccgccttcc
    24601 ggattcggca tcacctaccg ctttggataa ataataagga taatttatgc ccctcttaca
    24661 cctgctccgc cagaatccgg tgattgctgc cgttaaagat aatgccagcc tgcaactggc
    24721 catcgattct gaatgccagt ttatttccgt gttgtacggc aatatctgca ccatcagtaa
    24781 tatcgttaaa aagattaaga acgccgggaa atatgctttt attcatgttg atttgctgga
    24841 aggcgcgtca aataaagaag tggtgattca gtttttgaaa ctggtgaccg aggcggatgg
    24901 aattatcagc accaaagcat cgatgctaaa agcggcaaga gcggaagttt ttttctgtat
    24961 tcatcgcctg tttattgttg attcgatttc gtttcacaac attgataagc aagttgcgca
    25021 atcgaatccg gattgtattg agatcctgcc aggctgcatg cccaaagtgc tgggctgggt
    25081 gacggaaaaa atccgccaac cgctgattgc tggcgggctg gtatgcgatg aagaagatgc
    25141 gcgtaatgcg attaacgcgg gtgtcgtggc gctttccacc acgaataccg gagtctggac
    25201 gttagcgaaa aaattgcttt gacgggataa tcgtaaccaa ttgaatttgt tttgatttgt
    25261 tggccgcacg ccacatccga cattcagcgc ctgatgcgac gcttaacgcg tcttatcagg
    25321 cctacaggtc cccagtccag aaccgtaggc aggatcatcc ggcagcacgt tattggtatt
    25381 ctgcgtgaat gtttgtcacc agtgcctcca gcaccacttt ccagtcatca accacgtcaa
    25441 tatctgcctg tgaaaatacc gctgcgctgg cgtcatgatt aatcgccacc ataaacttgc
    25501 tattgcgtac gcccgccatt aacgctgctg cgccggaagc gccaacaaca atgcacacct
    25561 caggtgccag cagatgtccg gaaataccaa tcactttttc agcatcaacg ccaccgttca
    25621 tcactcgcgc ccgactgtag cccacttcgg ttcccagctt ttctgccagc ataacgattt
    25681 cctggttatc tgcctctccg ccctgcccca ccaccagcac ccgtctggcc tcagctaatg
    25741 ggtcgcggct aacatttttc agatcctctg tgctaaccag ccagtcggga agcgcacctg
    25801 ggacaatgtt cagttgctgc attccagagg gcaacgtggc gtttttcgct gctcccgcct
    25861 gacgcgccag ggaaaggcat aacgggcgtt tttctgtttg cagcgtcgcc gttaacgcat
    25921 ttccccagtg ggatttgcgc atgcttacgg ttggaatatc taacgaagtc acctgacaga
    25981 tgcttgcacc atgtaaacgc caggccagcc gggtcgacag ttcatcgcca aacgttcccg
    26041 gcggaaagag aacaacgtcc gcaggcgttc gctgccactg ttccaccatc gcatccagca
    26101 cctgttccgc cactacgggc tgaggttcta tttgccagtg cgccagcgta caaccgctga
    26161 aatcctgcgc cgccagccag ctcgctattg cggcattctc ctgatttatt gtcacaattg
    26221 cgatattcat ggctgcatcc tttggcgtaa atagtcctgc catagctttt gagctttttc
    26281 tgctaccgtt tgcccatcaa tcagcgtcgc tccgcatcgt tgctctgctc ttgccagttg
    26341 cagacactgc atcgctggca tttcggcagc aacagtttta cggataattt ctgctttccc
    26401 cgcagccatt cgctggcgca ttcccggtac tggcaaggcc acttcaccgc actgacgcac
    26461 ggcaatcacc gcaggcaagc gaacccggca acagcgcaac ccatgttcag tacgctgttc
    26521 gagggtgata aacagcgcgt cgagagtgaa acgctccacc tgggtgaagc agggccagcc
    26581 cagcatttcc gccagcaaaa atggcgtttg cccattctgc ccttcgctgc tttggcagcc
    26641 ggtaattatc agatccagcg gattctgacg ctgccattcg gcaatatggc gagcgacaaa
    26701 ttccggcgca aagcgcagat ctgccgccgt ctccagcaat acagcttcct caaacccaag
    26761 agccatgaga tagcgcagcc agtgcaacgc ccgttcatcc cccatgctca acgcggttaa
    26821 agacatcggc gtaccgtttt ttctctgcgc taacagcagc gcggcagcgg cctgttcatc
    26881 agcaccgagt aaacttcgca gtagcgaaac atccggtccg ctattaccct gagccgccgc
    26941 ctgccactct ttttccgcca gcattccggc atccggttcg gctttaaacg ctaacagaat
    27001 gttcattcgc acctcctaca tttcccgccg ccaccagcgg gagggcttta gtttctggtg
    27061 cccatagcca ggtcacaaca aaaccaacca acaacactgt cgccaggagc aataaggtga
    27121 cttgcattcc ccactgcgcc agcacccacg gcagcaggcc agtacttacc gccgcgccaa
    27181 gtcgactcat ggcggtggca aaaccgacgc ccagcgagcg aatgtcagta ggaaaacttt
    27241 ccgcaggcaa aatgcccacc agattactga ctgccgaaat ggtggtgctg aagagaacaa
    27301 aaagcagcag cgttaatgaa ctgccggaag gcaaacaggc cattactacc agcgttgccg
    27361 ccagcagcaa aaaacttccc agcaaaaatt tgcgatgtgc cagcaggtgc gtcagaacta
    27421 atcccagcag cgcgcccaca attaacaacg cattaagcat caggctggca gtcagcgcat
    27481 cttccagacc aatagtctgg gcgatagttg gcagccaggt ataaatcaca aaccatggga
    27541 ttacgaggca gacaaagaag acgctgttaa acgccgtgcg ccgccagtaa cgcgaagaga
    27601 acaaggtttt gatgtgttta tgggtcgccg ttaccacttc atcgcccagt aaaacatggg
    27661 gaccaaaata gcgatgcacg atagcgtgag cttctgcaaa acgcccctgg cacagtagcc
    27721 aacgtggcga ttctggcgtt ccccagcgta ataacgtaat caacaacgcg ggcagagctg
    27781 ccgatgccag caaccagcgc caggcctccg ggttttcgga aataaagtga tgtccggcaa
    27841 tacttgccaa cacatagcct acggtccaca ccacgctgaa tgcgcccagc aaaataccgc
    27901 gatggcggcg cggggaaaat tcagccagca aggtgtgacc tactgagtaa tcgcctccca
    27961 gaccaatgcc gatcaaaatg cgcagcccaa taagatgctc tggcgtggtg gcaaaaaatt
    28021 gcaagaacga agcgagcgta atcagcaaaa agctgaaggt gaagattttt tgccgaccaa
    28081 tatggtcgga gatccaccca agaaccaggc tgccaagaaa caaaccaagg agcgccgagc
    28141 cgccgatcat tcccgccata aacggcgtca gttgcatggc gggcgtaagt tgaataatgg
    28201 cgtaaccaat gacgccgaga acataaccgt cggtcaggtg tgcgccgaaa gtgagcgcag
    28261 caattcgaca gtgaaaacgg ttaagcggta aatcatccat tcgcaccggt gaagtgttca
    28321 tctttacccc ttcattgtcc ggtaataaga acggcttctc gcctgagaag ccgaaaaggc
    28381 aacgccccgt catcgcggaa cgctgcctga tacattattt ttcaatcgga tagatagtac
    28441 ccgtattcat aatgccgtta ggatcgaact gctttttcag cccttccagc aacgcccacg
    28501 cgctgccgtg ttccagcttg ctccagtgaa cgcgatgttt accaatgcca tggtggtgca
    28561 ccatcgaacc gccgaggcga atggtttctt cgcagatgat tttgttgagc ggattgtagt
    28621 acttgtcgat ttcctcttcc ggcttacagt caacaacgtt gtagtcatag acgaagtaca
    28681 tgttggtgcc gttctgatag ctatgagagg agtgaccgcc cagcatggtg atgtcgtcgg
    28741 cgtgcgggaa ctcagtacga atacggttaa taacgctttc gtagatttcg tggatgcagc
    28801 tccagcagcc ggacacttcg gtggtaaagc ccatgttgcc ggttttgagg atctgcacac
    28861 gttcggcagc cactttatcc ggtccccagt tcaggttgtt aaaccaggtt tcaatcagtt
    28921 tgctgtccac gcgctggcac tgcgggtagc gggcaacgat ttccgcaatc ccttcgcccg
    28981 tcgccttcgc aatgcgaggg ttaccttcag ccataaagat cagcacccat tttccgtcgg
    29041 caaaatgggt gaagtgttgg gtgccatctt cagcgtcata caaacgagcg atcgacggac
    29101 gatacccttc caccatgatt tcacgcagga tgttgaagcc ggttttcatg tcttccagga
    29161 tatagccgta gaagaggttg ttttccgggg tgtatttaaa gattttcact gttacttcag
    29221 tgatatagca caatgcacct tcgttgccga tgatgatgtg acgaatgtcc gggccagccg
    29281 cgcggcgtgg cacgttttta atgcgtgtga cggtgccatc tgccaacact gcctccagac
    29341 caacgaccat atcttcgatt gcgccgtaga gtgtggagaa ctgcccgata ctgcgggttg
    29401 ctaccaggcc gcccatctgc gccagcggct ttgactgcgg agaatgcccc gtggtgtaac
    29461 ctttttcacg caacgcgttt tccagcactt ccagcggaac accacattgc accgtcgcct
    29521 gcatattctc aatatcaata ttaatgattt gattcatggc ggagccgtcg agcaccaccg
    29581 agttttctac aacagtttcc agcccacctt cggtggcgga agcaccggta cgcggcacac
    29641 cgttaatttt gtgcgcattc ataaaattca gcacacggga tacttactct gtggaaccga
    29701 gttttacgac cgctgccgga atcggcaaag tataaatgcc atgaatatcc ggaaatttac
    29761 gaaaacggtc aatactgttt catcggtaat tacgcgatct gcaccaacaa tttcctttag
    29821 ctggtcgaca atcgctgcgc gagataaaga catagtaatt ccttcctgat aataaaaatg
    29881 gaataatcat taataagaga aaacagaaac caccgttatg gattagcgca ctaaataacc
    29941 gccatcgacc actaataaat gcccgttgac ataattcgat gcctgacttg cgagaaacac
    30001 ggctgcgccc attaaatcct gagtatcgcc ccaacggttt gccggaatat gatcaagaac
    30061 gcgctgattg gtttctggat tactgcgtgt cgccagcata atatgctgtt gcataatagc
    30121 caggggcgat accatttacc tgaatattat attgacctag ttcatcacaa taagctttgg
    30181 tgaacccggc aagagcatgt ttagtggcag aatatgcagg tgaccattgt ccacctaagt
    30241 aagagaacaa tgaacagata ttaataattt tgccgctttt ctgcgggatc ataatttttg
    30301 ccgcttcata gcttaactcg aatgcggcgg tcaggttcac atcaatcatc ggatcccagt
    30361 cggcacgacc gaagtccagc accttattca gcttacaaat accggcattg ttaaccagaa
    30421 tatcgacagt accgaagcgc tcacagcagg cagcgataat cttctgcggt gcgccttctg
    30481 cggtaatatc cacctgcatg aagtccactt caacaccctg tttttcaatc atttcctttg
    30541 tttcgccgtt atctttgacg aagctaggaa taaaaatatt tgcgccagct ttggccaacg
    30601 ccattgcaaa tgcctgacct aaaccgctat tcccgccggt aacaattgct gttttacctt
    30661 tcagggagaa aaaatccatt gagaacgcat tgagagattc gattgacata gttagctcca
    30721 atttccgtaa ggcaaaaaaa agaaaggtaa cttcccccgc gagagggaag ttacctttct
    30781 cattatctct ggtaactgga gataattttt agcataacgg cataactgct aatgtgatcg
    30841 taatcacagt gtgatattcg tattgtcaaa tagttttaaa aagcagaagc gtgattactc
    30901 tcacacaaca cctctgcttt ccatgttatt tcttattgca attactagag acctgagaaa
    30961 agtaatccac ctgaaagggc ggcttacttt tctctttttt ttgtctatta caaatgggaa
    31021 tacgatatgc aacacaactc atatcgccgt tggataaccc tcgcgataat tagttttagc
    31081 ggcggcgtta gttttgacct ggcttattta cgttatattt atcaaattcc catggcgaaa
    31141 tttatgggat tcagcaatac cgagataggt ttaataatga gtacctttgg tattgcggct
    31201 attattcttt atgcccccag cggcgttatt gccgataaat tttcacaccg caaaatgatt
    31261 acttccgcga tgatcattac cggattactg ggtctgttaa tggcaacgta tccaccgctg
    31321 tgggtaatgc tctgtattca ggtcgccttt gcgataacga cgattttaat gctgtggtcg
    31381 gtgtcgatta aagccgcctc gttgcttggc gatcatagcg agcaagggaa aattatgggc
    31441 tggatggaag ggctgcgcgg cgtcggtgta atgtcgctgg cggtgtttac catgtgggtc
    31501 ttttctcgct ttgcaccgga tgacagcgcc agcctgaaaa cagtcattat catctacagt
    31561 gtggtttaca tcttgttggg gattctgtgc tggttttttg ttagcgataa caacaacctg
    31621 cgcagtgcca ataacgaaga aaaacagtca ttccagctta gcgacatcct tgccgttttg
    31681 cgtatcagca ccacctggta ttgcagcatg gtgatttttg gtgtcttcac aatctacgcc
    31741 attctgagtt actccaccaa ctatctgacc gaaatgtacg gcatgtcgct ggtggcagcg
    31801 agctacatgg ggattgtgat caacaaaatc ttccgcgcgc tgtgcggccc acttggaggc
    31861 attatcacca cctacagtaa agtgaaatcc cctacccgcg tgattcaaat cctttccatc
    31921 atcggtttgc tggcgttgac agccctgctc gtcacgaact ctaacccgca atcggtcgcg
    31981 atggggattg gcctgatttt actgctggga ttcacctgtt acgcctcacg cgggctgtac
    32041 tgggcctgcc ccggcgaagc gagaacaccg tcttacatta tgggcaccac ggtaggtatt
    32101 tgttcggtga ttggattcct gccggatgtc ttcgtttacc cgattatcgg ccactggcaa
    32161 gacaccctgc ccgctgcaga agcctaccgc aatatgtggc tgatgggcat ggcggcgctt
    32221 gccatggtga tagtctttac ctttttgctg ttccaaaaaa ttcgtactgc tgatagcgcc
    32281 cccgcaatgg ctagcagcaa gtaagccagg aaaattacag gagaaaaatc atgtcgaaaa
    32341 aatacatcat agggattgat ggcggaagtc agagcacaaa agtggtgata tacgatctgg
    32401 aaggtaatgt ggtttgcgaa gggaaaggct tattgcagcc gatgcacacg ccagatgccg
    32461 atactgcaga acatcctgac gacgatttat gggcatcatt atgttttgcc ggtcacgatt
    32521 tgatgagtca gtttgccggg aataaagaag atattgtcgg tattggtctg ggatccatcc
    32581 gttgctgccg tgcgttattg aaagccgatg gcacgcccgc tgcgccgttg attagctggc
    32641 aggatgcacg cgtaacgcgt ccctacgaac acactaatcc tgacgtggca tatgtcacct
    32701 ctttttcggg ttatctgacg catcgcttaa ccggcgagtt taaagacaat atcgccaact
    32761 attttggtca gtggccggtg gattataaga gctgggcatg gagcgaagat gctgcggtaa
    32821 tggagaagtt taatatcccc cgtcatatac tgtttgatgt gcaaatgcca ggcactgttc
    32881 ttgggcatat cacaccacaa ggcgcgctgg cgacacattt cccagctggg ctgccggttg
    32941 tttgtaccac cagtgataaa ccggtagaag ctctgggggc cggattactg gatgatgaaa
    33001 cggcggtgat ttcactcggt acttatatcg cgctgatgat gaacggcaaa gccttgccga
    33061 aagatccggt ggcgtactgg ccgattatgt cttctattcc gcaaacattg ctgtatgaag
    33121 gttacggtat tcgcaaaggt atgtggacgg tgagctggct gcgcgacatg ttaggcgagt
    33181 cgttaattca ggatgccagg gcgcaggatc tttcaccgga agatttactc aacaagaaag
    33241 cctctagtgt gccacctggc tgtaatgggc tgatgacggt gctggattgg ctgaccaatc
    33301 cgtgggaacc gtacaaacgc gggattatga tcggctttga ttccagcatg gattacgcat
    33361 ggatatatcg ttcgatactg gaaagcgtgg cgctgacgct gaagaacaat tacgacaata
    33421 tgtgtaatga aatgaatcac tttgcgaagc atgtgatcat tactggcggc ggttcgaaca
    33481 gcgatctgtt tatgcagatt tttgccgacg tgttcaacct tccggcacga cgtaacgcca
    33541 ttaacggttg tgcaagcctg ggggcagcga ttaatacagc ggtaggtctg gggctatacc
    33601 cggattacgc aacggctgtc gacaaaatgg ttcgcgtgaa agatatcttt atgcctgttg
    33661 agagcaatgc caaacgctac gacgcgatga ataaaggtat tttcaaagac ctaaccaaac
    33721 atactgatgt gatcctgaaa aaatcgtatg aagtgatgca tggggaattg gggaatgcgg
    33781 attcgatcca gagctggtcg aatgcgtaag tgaggaagac cgggcgggaa actgcccggc
    33841 ctgaatatac ctgaatggtt atccccgctg acgcggggaa ctcgaataac tgaccaacgt
    33901 ctggctctct aaatccggtt tatccccgct ggcgcgggga acactctaaa cataacctat
    33961 tattaattaa tgatttttta agccagtcac aatctaccaa ctttacagga tcacacaaac
    34021 aacacatcca ttatgttaaa gagcacttaa tccattgatt aaaaaggtaa atatttaaaa
    34081 taactctata caaactaaaa tctaccaaac ttcgccgcaa taattttcac tccagcaaaa
    34141 aattaacgcc gcagaatttg tagagaatga taatgattag tccgggatgg gcaagatgga
    34201 tgagttttca ggggcgacaa ataatacaaa tgagtggaat tttttacaag ttccacccat
    34261 ttataa
//
LOCUS       DAJTYG010000037        33599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTYG010000037 DAJTYG010000000
VERSION     DAJTYG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.037"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            582..719
                     /locus_tag="OO568_001879"
     CDS             582..719
                     /locus_tag="OO568_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641243.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1319..2098
                     /gene="ipaJ"
                     /locus_tag="OO568_001880"
     CDS             1319..2098
                     /gene="ipaJ"
                     /locus_tag="OO568_001880"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR6641244.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3046..3975)
                     /gene="virB"
                     /locus_tag="OO568_001881"
     CDS             complement(3046..3975)
                     /gene="virB"
                     /locus_tag="OO568_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR6641245.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4192..4428)
                     /locus_tag="OO568_001882"
     CDS             complement(4192..4428)
                     /locus_tag="OO568_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6641246.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4432..6333)
                     /gene="ipaA"
                     /locus_tag="OO568_001883"
     CDS             complement(4432..6333)
                     /gene="ipaA"
                     /locus_tag="OO568_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR6641247.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLIDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRTNNKLKVTDANKINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSQIDDTSAELLTDDISDLKNNNDITAENNNIYNAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6342..7340)
                     /gene="ipaD"
                     /locus_tag="OO568_001884"
     CDS             complement(6342..7340)
                     /gene="ipaD"
                     /locus_tag="OO568_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR6641248.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNKALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRK
                     GYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7391..8482)
                     /gene="ipaC"
                     /locus_tag="OO568_001885"
     CDS             complement(7391..8482)
                     /gene="ipaC"
                     /locus_tag="OO568_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR6641249.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSAQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSI
                     SSNAVSLIISVAVLLSALRTAENKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSAASQIAGNIRA"
     gene            complement(8502..10244)
                     /gene="ipaB"
                     /locus_tag="OO568_001886"
     CDS             complement(8502..10244)
                     /gene="ipaB"
                     /locus_tag="OO568_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR6641250.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINR
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10250..10717)
                     /gene="ipgC"
                     /locus_tag="OO568_001887"
     CDS             complement(10250..10717)
                     /gene="ipgC"
                     /locus_tag="OO568_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR6641251.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10774..11400)
                     /gene="ipgB1"
                     /locus_tag="OO568_001888"
     CDS             complement(10774..11400)
                     /gene="ipgB1"
                     /locus_tag="OO568_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR6641252.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11416..11805)
                     /gene="ipgA"
                     /locus_tag="OO568_001889"
     CDS             complement(11416..11805)
                     /gene="ipgA"
                     /locus_tag="OO568_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR6641253.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11818..13302)
                     /gene="icsB"
                     /locus_tag="OO568_001890"
     CDS             complement(11818..13302)
                     /gene="icsB"
                     /locus_tag="OO568_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR6641254.1"
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGDVTKDTEINYLFEKRLGYSVDTYKQDKLNMISDQTAERLDAGQEVINLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDVVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13616..15232
                     /locus_tag="OO568_001891"
     CDS             13616..15232
                     /locus_tag="OO568_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR6641255.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVYYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESEHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15242..15604
                     /gene="ipgE"
                     /locus_tag="OO568_001892"
     CDS             15242..15604
                     /gene="ipgE"
                     /locus_tag="OO568_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR6641256.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15604..16062
                     /gene="ipgF"
                     /locus_tag="OO568_001893"
     CDS             15604..16062
                     /gene="ipgF"
                     /locus_tag="OO568_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR6641257.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16071..17177
                     /gene="mxiG"
                     /locus_tag="OO568_001894"
     CDS             16071..17177
                     /gene="mxiG"
                     /locus_tag="OO568_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR6641258.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKKIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFFR"
     gene            17195..17446
                     /gene="mxiH"
                     /locus_tag="OO568_001895"
     CDS             17195..17446
                     /gene="mxiH"
                     /locus_tag="OO568_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR6641259.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17459..17752
                     /gene="mxiI"
                     /locus_tag="OO568_001896"
     CDS             17459..17752
                     /gene="mxiI"
                     /locus_tag="OO568_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR6641260.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17758..18483
                     /gene="mxiJ"
                     /locus_tag="OO568_001897"
     CDS             17758..18483
                     /gene="mxiJ"
                     /locus_tag="OO568_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR6641261.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18480..19007
                     /locus_tag="OO568_001898"
     CDS             18480..19007
                     /locus_tag="OO568_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR6641262.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18949..19644
                     /gene="mxiN"
                     /locus_tag="OO568_001899"
     CDS             18949..19644
                     /gene="mxiN"
                     /locus_tag="OO568_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR6641263.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALSKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19637..20044
                     /gene="mxiM"
                     /locus_tag="OO568_001900"
     CDS             19637..20044
                     /gene="mxiM"
                     /locus_tag="OO568_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR6641264.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20028..20456
                     /gene="mxiM"
                     /locus_tag="OO568_001901"
     CDS             20028..20456
                     /gene="mxiM"
                     /locus_tag="OO568_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR6641265.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20586..21218
                     /gene="mxiE"
                     /locus_tag="OO568_001902"
     CDS             20586..21218
                     /gene="mxiE"
                     /locus_tag="OO568_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR6641266.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21205..22905
                     /gene="mxiD"
                     /locus_tag="OO568_001903"
     CDS             21205..22905
                     /gene="mxiD"
                     /locus_tag="OO568_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR6641267.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22924..23991
                     /gene="mxiC"
                     /locus_tag="OO568_001904"
     CDS             22924..23991
                     /gene="mxiC"
                     /locus_tag="OO568_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR6641268.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSQASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24004..26064
                     /gene="mxiA"
                     /locus_tag="OO568_001905"
                     /gene_synonym="virH"
     CDS             24004..26064
                     /gene="mxiA"
                     /locus_tag="OO568_001905"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR6641269.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26077..26478
                     /gene="spa15"
                     /locus_tag="OO568_001906"
                     /gene_synonym="spaK"
     CDS             26077..26478
                     /gene="spa15"
                     /locus_tag="OO568_001906"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR6641270.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26482..27774
                     /gene="spaL"
                     /locus_tag="OO568_001907"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26482..27774
                     /gene="spaL"
                     /locus_tag="OO568_001907"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR6641271.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27867..28205
                     /gene="spa13"
                     /locus_tag="OO568_001908"
                     /gene_synonym="spaM"
     CDS             27867..28205
                     /gene="spa13"
                     /locus_tag="OO568_001908"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR6641272.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28192..29070
                     /gene="spa32"
                     /locus_tag="OO568_001909"
     CDS             28192..29070
                     /gene="spa32"
                     /locus_tag="OO568_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR6641273.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29064..29945
                     /gene="spa33"
                     /locus_tag="OO568_001910"
                     /gene_synonym="spaO"
     CDS             29064..29945
                     /gene="spa33"
                     /locus_tag="OO568_001910"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR6641274.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29950..30600
                     /gene="spa24"
                     /locus_tag="OO568_001911"
                     /gene_synonym="spaP"
     CDS             29950..30600
                     /gene="spa24"
                     /locus_tag="OO568_001911"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR6641275.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30615..30875
                     /gene="spaQ"
                     /locus_tag="OO568_001912"
     CDS             30615..30875
                     /gene="spaQ"
                     /locus_tag="OO568_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR6641276.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30875..31645
                     /gene="spa29"
                     /locus_tag="OO568_001913"
                     /gene_synonym="spaR"
     CDS             30875..31645
                     /gene="spa29"
                     /locus_tag="OO568_001913"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR6641277.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31652..32680
                     /gene="spa40"
                     /locus_tag="OO568_001914"
                     /gene_synonym="spaS"
     CDS             31652..32680
                     /gene="spa40"
                     /locus_tag="OO568_001914"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR6641278.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32693..32935
                     /locus_tag="OO568_001915"
     CDS             32693..32935
                     /locus_tag="OO568_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641279.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10481 a         4887 c         6730 g        11501 t
ORIGIN      
        1 tggatctgat ttcaggcgtt gggtgtggat cactattgca ccgttcgtga cacttgttga
       61 ctacctgtgc ggcatctggt atcaaggtgg cgtgaagccg gttcaggttc tcatgcggaa
      121 cgttggaacc tatatccccc tgataccaaa ggcgaaatgt aagtgacaaa atgcaatgaa
      181 cacgttctcc gttaactgag gagcatggtg ataaataaag gtaaatgcag aaggtgtaga
      241 gtctcgcaga tttctgacat catagagcct aacattagat gcataacgct acagattgta
      301 gaatctctac aggtgaaata tacactggtt ggcgtttatg tcgcaaaacc tgtcattcag
      361 gttcatctca ccaataagga tatgagtgaa gtggaggata agtgagtctg ctgtcagagt
      421 tttctggtgt atgtcagtaa ctgcgaacca tgtctgcttg atatgggagc ttatggaggc
      481 tctctgtaca aggttaggga gctaacaaag cagggataaa aagtccgcct gatgcaggca
      541 cggttcgtta aatcttttgc cattggtaac aaaatggtgc gatgaatgtt caggctatct
      601 ttatcttgat ggtggttcag ctgtttggta aagaaatcgc tgtaacaata gaagaactgc
      661 aggcagtact ggtcccacat cgtttgctca gtcaactggt gaagttccgt agcgcgtaaa
      721 ttaatgcttt gcatgaaacg ttactggagt ggatgaaaga atacctgcat atatcattac
      781 tgcgttggat taccagtaca acagattgaa tgaactggac atgttgatag acggcattga
      841 aaagcagctt ataagcatag tgcggcagag ttaattggag agcacttcgc cttctgagta
      901 ttaagtaagc atggtggtac ctgcggatac tatctattca ctgtgctcgg tcggcagcct
      961 tactgaccaa ggagttgggg gagttgataa caaagttgaa aaaacagctc tcaaccaagg
     1021 tggttattgt aacaatagca aataagctgg tgtgacctgt atcacaataa ccgattatgg
     1081 acgtaaatac gacaaaaata tgtcagtatc aggcggtatt aattgctgat gctgatggac
     1141 attaactctt aataaaaggg tggatgctga aaggttgtta tggtgacgga cgtgatgacg
     1201 acggaaaaga gtatgaggtt ttggatttaa agttcgttcg gggaataagg acccggtcag
     1261 ctaattctca tagtagttcg cattgctgga tgcaatactg tgctgcaatt actcgtcgat
     1321 gtcggaacaa cggaagcctt gcaaacgggg atgtattcat acaggtgtta tgttatatgg
     1381 ggttttattg cagggggcta taccaagaga atatatgata tctcatcaga ctgatgtgag
     1441 ggtaaatgag aatagagtaa acgaacaagg ttgttttttg gcgaggaagc agatgtatga
     1501 caattcttgt ggagctgcca gtttactctg tgctgcaaag gaattagggg ttgataaaat
     1561 accacaatac aaagggtcta tgtccgaaat gacaaggaag agttctcttg atttggataa
     1621 tcgttgtgag cgggatttat acttaataac tagtggtaat tataatccga gaatacacaa
     1681 agataatatc gcggatgccg gttattctat gcctgataag atagtcatgg ctacgaggct
     1741 tcttggattg aatgcttatg tcgttgaaga aagtaacatt ttttcgcaag taattagttt
     1801 tatttatccc gatgcaaggg acttattaat tggcatggga tgtaatatag tacatcaacg
     1861 agatgtgtta tcttctaacc aaagagtttt ggaagctgtt gctgtatcct ttattggagt
     1921 tcctgtcggc ctccactggg tgttgtgtag acctgatgga agctatatgg atccagctgt
     1981 aggagaaaat tattcttgtt tttcaactat ggaattaggt gcaagaagaa gtaattctaa
     2041 ctttatagga tatactaaaa taggtatttc catagttata actaatgagg ctttgtaaat
     2101 tgtcgttttt tatctgtgaa ggagcttgaa tattttgggg cggggggggc ataacaaatg
     2161 atattactga gtacaaattt agctatattg ggcggattag ttgtgtggga agacgaaatg
     2221 tttctcttga aataagtgaa ctccctcaag gaatgattgt gtgcttaaat atttaagtgc
     2281 tggtaagttt gagaaggctg acatattttg cagagcaaac agtgattgac ttggatgata
     2341 tttttatttt agtcactcgt tgtaattgga gtatgcttta ttttatttga aaatagatgt
     2401 attttatacc cgcaatttaa actgtaagcc ctatgagggg tatggatatt ataagttact
     2461 ctttgactaa gcataacttt tggggttaga aaaataaatc tcttgttaat aattaatatg
     2521 attttaaaat caaacttgtt tataattata aaataatgta agtcggtcat tttatgggaa
     2581 gattaggaga ataaaacagt aattatattt gagaaatctg ctaatatatt cttaaagctt
     2641 ttttgatact gtgaacgaag tctgttaatg gtggattttt tgtttatcac catgtaaaaa
     2701 aattgagtag taaaagcgtt gactgctaat cctcgctgtt tttattgaaa cgattaaaaa
     2761 tacgctgaat attttcggcc agttactcat cttcacataa aattttgacc atcatgccgc
     2821 atcccttagt tgcacaattc attttcaatg atatgaatat tactaatttc accagataat
     2881 tttctcgtgg tcagcagggt atgagttttc accgtaattt taaggaaaca cagtgcgtgg
     2941 ggattgaata acagatagcc gacggaatta ccccaccggc tgaaagtcaa gttcaacgaa
     3001 gatttttatt atctgaattg ggcagtttac atcagtgttc gatgtttatg aagacgatag
     3061 atggcgagaa attatatccc gaatagcttc atctatttct ctctgaactg ttttgtttat
     3121 tttagataat gtaaatatta gagttctgtt tgttttattc tcttttcttt ttatgtaagt
     3181 ccgtttgtct ttagaaataa ataacgagtc aacttttagc gaagggtttt gctttctgtt
     3241 tttgcttttc tttatgatgt ttaaaatttc ttttttatat atgtccgggg gcaaatttgt
     3301 atcaaggtct tttatttctt cctttaatat tggtagtgta gaactaagag attcattagc
     3361 cttttcaagt cctttataat aattgaataa tatcttgtag tcattaaagt taagttctga
     3421 cgcgattgga aatagagata ttatttcctg tggaacgctt gctgcctgaa aggcacgagt
     3481 gactttcgcg cgagacagat tctctttttt ggctatgtct ttataggaca tccctgatac
     3541 tttcagaaaa ttaagaccaa taccaagttc tcggatgcta tgctcttttg ctgtttgtat
     3601 atcgtttgct agttttctgg catcaagagt agatatatat tcttttgaat atagaacttc
     3661 aagatctgct cctgcatata ttgcagatgc tcttctacga gtgccatcca gaatttcaat
     3721 tctaccatca atctcccttc ctattacagg gaagaattgt tgtagcttta tagtttttat
     3781 gatatcggct aatgattctt ctgttaggga ttcttgatcg cgtacattcg ttttttggtt
     3841 gacgaaggtt aaatcttgga tccttttatg aggaatcttg gctttgataa aggaaacttt
     3901 attaccagaa tggagtgtaa actctttctt ttggccttcc tttatactta acaagtcgtt
     3961 gcacaaatcc accatatcac accctgttta ttcatattga tttatattga caaaagttaa
     4021 atgcagtgtg agcaagattg tatgtaactg ttgatgatag tcaacggtag tcttaaaaat
     4081 tactatttta atagctggat tgatgcaatt tatctttatt ggataaaaga ataatattct
     4141 tgtggagctc tgatgtgaga gctccataga tgaaaaaatg cttatcaatt actatattga
     4201 ttttgtacta tttttgacta cagaaataag atctccaata gtaataatgt gttctaatgc
     4261 agattcatct atacgtagat tgaactcaaa actaatcata tccagcattt caatcagaga
     4321 aagagaatcg gcagaaagat cttcaataaa tttagtttct tcagatagtt tgctgtacgc
     4381 tataccatag caaaaggcca ctattgataa tattttttct tttatcatat tttaatcctt
     4441 attgatattc tttaatactt ttgataggga agtggttaca tcttttgctg cgttatatat
     4501 attattgttt tcagctgtaa tatcattgtt attttttaaa tcagatatat catctgtaag
     4561 taattctgca gaggtatcgt caatttgtga caataccttg ctgagggcgc tagatacttc
     4621 ttttgccttt tcatagatcg catgattttt atcaattgta tctgcgttgg tttctgttac
     4681 gtagtttatt ttatttgcat ctgtaacttt taatttgttg tttgttctgt gttctgttgt
     4741 ggattttgta atgtcctttg atttttcctg taactctcgt tcttgtgttc ctgatttagt
     4801 tccaatgaga gatataagat tacttaggga gtttgttact ttttttgaag cttcaaatat
     4861 cgtttcccgt gttaaatatg tgtctccatt tcttgataaa aaatcagtct gaagtacttt
     4921 tgaatcagtg tttgagttga atttatttat ttgcgaagct actctatgag aaagagaagc
     4981 gctactttta gttgtatctg atgagttatc attactgctt gtcactcctt ccgggtggag
     5041 agggggggtg gttgaagagt tctgtatgtt cagaggcctg tgtccccgag aaagagagtt
     5101 atttgcagat ctttcttgac tactcgtagt taacaggtct gaacctattc catccgaatt
     5161 atctggagta tggttgaaaa ccttttctaa agctgaatta tttgcaaatg tctggacagt
     5221 atctgatata gagtgtggta gaacggaagt tactttttga actagtgctc tttgtggatt
     5281 tagtaatgag tttccgttac gggataatag atcgtttgtc tgagaagtag gagactgtgc
     5341 atcattttct gggttctcgt gatagctatt gtcatatact ctgttgtcaa aaactctatt
     5401 atcgtaagtt ctattatcat tatttatatg atagtgaatt acaggttggg atgttttctc
     5461 attggctccg ggggttggtc cgccatctgg catatcttcg ggaggtgtag ggaggccatc
     5521 ttggatagga tgcgaatctt ttatatcttc taaaatatca ttaaggactt ttctcaacag
     5581 ctcaggaaac acggctactg tatttaagtc tcttgacagt ttacctatac caaatccttt
     5641 actatcaata gtatttttac gttgattgcg caaactatca aaggcaagtt ttgcctgtgc
     5701 gtcaattaaa tcatctagtt tgagagatga aacagtttca ttacttaact ttaattcagc
     5761 aagaatatta ttcttgataa atgatgatac ttcagtattg aggagttttt gtcccaatga
     5821 taaagcttca ccagatgtgt gttgttgtgc taatagtgca agtctgtgct caatgttttg
     5881 tcgtaattga tggagtttct tattctttgc cgaccatggt ccaaaggcag aatcttggga
     5941 taatttatct tttataatta gttccgaata ctgtgcgaca attttttgaa gtcccttttc
     6001 gttttcaaaa ttaagaccat tttgatgtac taaggagtac aaaacatttg aaaattcgag
     6061 aatctcgctt ttggcatacg cattcattga gttggaataa atgcttctta acacatcttc
     6121 tttccctgag gaagaaaata aaaaatcaag acatttctga ttaaaagacg ttgcaaagtt
     6181 ttctttttca gacactgatg ctggtgtttt tagagaagtt tgcgaactat ggatatcgga
     6241 tattttgctt tttaactcgc tgtattctgt tgatgatggt gaagttgcct tatataagaa
     6301 tgttggcgct tgagtattat ttacattatg catggcgcac ctcagaaatg gagaaaaagt
     6361 ttatctgtat ctgtacatga gcttattgta ctactcaaaa cctttactaa attatcaaaa
     6421 atactattgg cattactgta tttttgaact aaagtttgaa gattattttt cattgtttca
     6481 tcttcggcag agaatccggc attccatgcc tgatattttg cattatctag cacaacctcg
     6541 ccatttccac ctagattatc taagcttttt aacatattgt ctattggggt catgtttata
     6601 ctgacaacat atcccccgtt tttttgagat accttgccga ttgttccacc taattctgta
     6661 agccatttat ttgcttgttc ctgactaaca gtattatttg ctggatatag cggtttatct
     6721 ttatattttt ccttgagttc ttccaatgcc tttttaagcg agttgacttg taatttcacg
     6781 gagtttccgt cgttacctcc gggagagatc cagccggcaa gactggaaag aacagcgcta
     6841 aaatcttgat acatttgagt atatgaacta acggcatgtt catatacttt cagatactgt
     6901 tcattaatat cattgatgga gtttgcaatt ttagcccaca gttctctatg agatatcatt
     6961 tgatctccat caagctcggc ttctttcggg gctgaatgta ataattctct tgcgtcttta
     7021 ttaattggat atcctttcct ggaaagaata tccaatagtt gagcggattt atttacatcc
     7081 atgctaatct gagatgaatg taatgctatt tcttctagcg atgttttagt caacgttttt
     7141 tgtgaaagct ctttctttaa tgccttattt gttgttctga tattatgaag ggtgtcgtta
     7201 agcatagtaa gggaacttac aggatgagaa ctggtcgttg tttttatatc agaattaact
     7261 gtttcggttg atgaaccgtt ggtattgttt ggactgaatg atgaggtgga aatactatta
     7321 gtcagagttg ttatattcat ggttatttcc ttacaggaag agccatatat tattatggct
     7381 cttggatata ttaagctcga atgttaccag caatctgact ggctgccgaa ttccttgatt
     7441 gggtgatgct gtcaattata ttcaataatt tttgtattaa ttgatttgtc gcttgggatg
     7501 cttctttaga tacttgggat gcttcctctg cttgtttact gctggcctga ctgattagtt
     7561 gttcttcttc ttcaagagca gatgcataac gccctcctga tgtggatata ttaccagcaa
     7621 cggctgatgt ttccattgtt gcacggccaa tgttttgttt ttgctgcgca gaaagggtat
     7681 tgagctcgta agttcttctc tgggtgtcaa ttttatcctg caaagaaata tcgggagaac
     7741 taagagaagt tttatgttca gttgacaggg atatattatc tggcgccagt ttatttttac
     7801 ctaaaaattt tgtgctagag ttagtttgtg gtgaggtgat atttgtatct atttgtttat
     7861 ttaaccctaa tttagaacct gcaagctctt tttcaagaga ttgagcagtg gcaaggttct
     7921 ttcttaaggc tcctttttgg tcgctaatcc ctgaatgcgt ttttttggca ccgatacccg
     7981 ttatacctac ttgtgtgact gctccagtaa tgcttgatga tagggctgcc aggccttgcc
     8041 gaacaatgtt ctctgcagct gattttgtag catcgaacgc aatcaatgac aattgagagc
     8101 ccaatttatt ttctgcagtg cggagagcag aaagtagaac ggctacacta ataattaaag
     8161 aaacagcatt agaagaaata ctggaaatat ccagagtgtt ctctggagta agggtgtgac
     8221 tcatctctga aagatctttc atctttttgt cagtaaggcg agcaatgatt tctgaatcat
     8281 gctgaacctg cgctgataac tttaaaagtt gatcatcatt taatgtggtt gttaatacgg
     8341 gatttttacc gacattaagt ggaatatgcg ctgcaatctg ctgataattt tgtgattttt
     8401 gtgtttcgga agaactttga gtttgttttg tggatatatc tgtatataaa atctgggttg
     8461 gttttgtgtt ttgaatttcc ataacattct ccttatttgt atcaagcagt agtttgttgc
     8521 aaaattgctt ttgcaacatc agttctatta gcctgagagt tggacattga ggctaataga
     8581 tctgcaatta cttcctgcaa ttggccgaat ttttctattg cttcactgat atattttgac
     8641 agttgttcaa cttgatattt cgataatgtc aggtctgcta gatttgtcga cgcgctgttc
     8701 tggaaaacag cagaagcgac acttcctccc gcttgtgtgg cagagttaac actttctcct
     8761 aataaaactg cttggtttaa atgggtggaa ataatttgtt tggatattac ttcatcaccc
     8821 gctgcaccaa gaaatttatt taatctggca acagcattag tgattaaatc gtccagttga
     8881 gaagaaaaat tcttgagaaa ctttggtata aggtctgtga gggttttacc tattattttg
     8941 ccaatattct ctgcaagttt tgctgctgcc tgtttaccaa cagtggctac gagaacgact
     9001 gctgcaacta ggacaagagc gcctgcgatt gcccccagaa tagagccaat cattttggct
     9061 tttttcgagt cgacgcccaa gccttcgagc atttttgtaa atgcatctga aaggagtttg
     9121 attaagggtt caatgactgc tttcatgatc ggattcaggg cttgttccat gaaggaattg
     9181 ccggtcgctg cttgtactat agcatccgta accataagag ctaaaccaac agctgccagt
     9241 gctagagagg ctcctccaga aaaagctgct gcaacaacac taacgatagt taaaagtgcc
     9301 ccaagtattt tcccaacaca acccattact ctgttgagtt cttctgcttt acgtacttca
     9361 gcagcatact catcagattt tctctccatt tcagtttttc ttgattcttg gagagactgg
     9421 aatagagcca gatcattttt taaagattct tcattatttt ttccaactag ttgaataaag
     9481 gttgccatca attgagtaac actggcaagt ccggttaatg atttctgctg ggttgatagc
     9541 tgttcagcag atgctgtgtt tgaaaatgca gaaaaagagt ctatttcttt ttcgagttgc
     9601 attgatttat ctgtaagttt gctatgaatt gacagggttt tctgctcaat caatgtcctg
     9661 tctttaactg ctgcgtcttt tttgatagtg agttgtattt cttcccggct taattttttc
     9721 ttttctggtg actctgggtc gagttcggat aatcttgttt gaattcggtt aattttattt
     9781 tctaggtctt ttattttaga atctgcgttt tttagtttat taatttgttt ttcatagtct
     9841 ctggtaagtc cttcagtttc agatagaaga gtgttaattt tatcggagaa ttctaggttt
     9901 ttttgctgtc ttgcctgttg ctgggacttc caagcagtaa ttttatttgt taatgcagtt
     9961 aaagattttt caccgagtat ttgaataagg tttccaatta aaagcgttag ttgggaactt
    10021 gcatttaatg actttggtgc tttaagttca gggattaata tatttgaagt tgagtgtgca
    10081 ttagttgtat taatattttg gttagcagta agatcagcaa ttgtaagaga aaataatttg
    10141 ttagctgcat catttgcagc ttggatagta ttgtctccaa gctcagtgga agccaatatt
    10201 ttggcaagag gaaaaccagt ggttgtggtg cttacattat gcataataat tactccttga
    10261 tatcctgaat tgcgtccaag tatgattgtg cttttatttt taatttttca tcattgctgt
    10321 gttgaattac gagttcgaag cactctttag cttttaaggg ggcttttaac cgaagctggc
    10381 attgtccagt atggaatact ggtgtatagt cattttttcc taatgcaaaa gcgacagcat
    10441 aaaggtctgc tgcttgttgg aactgttctt ttatctgata aatagctgcg agtcccataa
    10501 tgtagtctac attgtaaaag tcgtatatac ataaaaatct gaagaaaact tcagcttcct
    10561 ctattcttcc tttgttgtaa aagtcataag catatgaata aatgtcatcc atcatatcat
    10621 caggaattgc attaatatct ttcagtgtag cgccagagtt aattgcatca attactgcag
    10681 tagagatgct ttcattttca gtgatattta aagacataag gtctcctttt tgtctgcttt
    10741 actttaataa gtataagatt taatataaaa gatttaattt gtattgcttt gacggtatac
    10801 agcctttaga acctctgcgc ttatttctat ttgtaaaact tttgttgatt cattaaatat
    10861 tttatcgtag acggaactgg atactagatt tgtaagatcc ttcatttgtg tgttaaacaa
    10921 atccatgtca ctgctatagc gttttaaaat ctttgctctg aggccagatg aggcaatctc
    10981 gtttagtgct gtatgactga tagatgtttg tgctgatttt tcatcaagtt ttatatccgg
    11041 gaattttttt tcaatagctg caaatatgtt ttttttgtta tctgaggtca aaacaactcc
    11101 attgtgtatg gccatttcat ctatacttct gtttatcatt gaagagacat aagaggttcg
    11161 ctcttggctc atccaacaaa atagttgatt ccccgaatta ctatctttta ttttgtctat
    11221 gacgctgtcc ctgattttat ttatattcac cgggcatcca aaattcttct gaggaaagcg
    11281 ttgttttaag ttaaaaacag ataatatttt ctttacaagc ttattgtgac ttaacgaatc
    11341 aaatgatggg cgaggaatcg cgaattcaac ctgtggaagt attttgttta gaatttgcat
    11401 gataccccct atatgttagt tcacttctga agtgatgttt gcaatggtta gtttaataca
    11461 aaataaaaat tcatacaaag attcaatgag ttcatcaggt gaaagatatt ttttgtagtg
    11521 tttcttggtc aaaacacatt gatgagtatc agggagaaga cagatagaag aaccataatc
    11581 tctactcaaa gagtagttaa ggatgagtat gtgttctgga ttaataatat tttctgtaaa
    11641 aagttcatgt tcattaagaa gtccggtcaa aaagatgtat tcatcattat ctgttataca
    11701 aacaggtatt tgttcttcga gcaatataaa ggcttgattt ttatcgttaa aatcaagctt
    11761 tgcacctgtt atttcataaa gtttatcata tagtttgcga cacatttcta tggccttcta
    11821 tatattagaa tgagagttat tcaataaaag cttattaatc tgtaataagt cgtgaatgta
    11881 aagtttggtt ccaagatctg gcgatttaag agaattgtaa taatcaataa tttttattga
    11941 tttttcgata atttcttctt ttttatctgt tgcttcggtt tttaattcgt ttaatacacg
    12001 agtcatattg ctgggtgctg attttactaa gtatgagtgg caagcgttga tgaccacatc
    12061 aaagtcttct gaagatacat tttcagtagg atgtttgtaa agtgaaattg ataaaggctt
    12121 gaatgttttt ggtgtgtgga taaggttaat tttattgaaa cttttaagat agctgcgtcg
    12181 taattgagtc aatctggaaa gatcaaaatc agattcaata tacttcatta tttcgttata
    12241 agactgattg agactttcaa tgcgttgcct aactttattt gcatacgcgt atacatcgtt
    12301 aggtgtagca accaatttaa cgtcagggag tggaaagtat atttcagaat ttcctgcttt
    12361 aagaacatta agtgccatac cagcacagtt ttcagattta ctgattaatt tataatttgc
    12421 caattgtctg aaagcatctg ctttcttttt gcatataaat tttgacatgt ttgtttcatc
    12481 aaggccaaaa aggttatggc tgattttacc atcctttgtt ttattatcac ctgataatgg
    12541 aatgtatact ttatcagcac tgacccccca ataaccatag gggtcttttt tttgattagc
    12601 tctgggaaaa aatatttttt tgttgttatt ttgatcttgt tttgaattta ataagtttat
    12661 aacctcttgg cctgcatcaa gtctttcggc tgtttggtct gaaatcatat ttagtttgtc
    12721 ttgcttatag gtatcgacac tgtaacctaa gcgtttttca aaaaggtagt ttatttctgt
    12781 atcttttgta acatccccgc cgggatacca gctgagatag gtatgagttg gttcctgtag
    12841 taaattgttt tttatactga tactagtatg ccctaaatgg gttatgcctc tatttcccca
    12901 gcaacgaaaa actaaatgtc tttcaagatt tttggctgta ccgatgccat gaaaacgctc
    12961 tgcggtattc aacattaatt tgatgtctct acctgtgaaa gagtgttgaa ttgagttttt
    13021 agctgcttca taggcggcaa acgcactttc tgcagatttt tcgctagcta atgtattgag
    13081 gaaaagcaat aaggattttt gattctctag tcgataattt tttagttgtt catttaatat
    13141 ctgtgaattt atctttgcat taatagtgct taatgttctg aaaaagagat cctttaggtt
    13201 gaatttctgt gctatcaata taggtccatg ctctgtatct tcaacgcgta taggcccttt
    13261 tgtattgctt gcgtcaatga aattgctaat tttgaggctc atactttatt aactctccat
    13321 tacttggtga ttgtggggat gtaaaatgta tttggcatat aatatattca attaataaat
    13381 ttagaaactt gagcctgtta acataatcaa attttctttt gctgtacata atatgtacct
    13441 cgtgagcata tgtagtgctc gtttcatcat gaaatcccac aagataaagt gcctgatgta
    13501 tcaggctcgg agtgttatag aaaaagagag aaccctgttg aataagtatc ttttttgttg
    13561 taaaacgagg tacaaaactg caatcaatgc cccatttatg actgaggata attaaatgca
    13621 cataactaat ttgggattgc atcaggtttc atttcaaagc ggagattcct ataaaggcgc
    13681 agaagaaacc ggaaagcaca aaggtgtaag cgtgatttca tatcaaagag ttaaaaatgg
    13741 agaaagaaat aaggggatcg aagcgcttaa tcgattatat ttacagaacc aaacaagctt
    13801 aacaggaaaa agccttttgt ttgcgcgtga cagagctgaa gtttattacg aggcaataaa
    13861 gttggcaggt ggtgatacgt caaaaattaa agccatgatg gaacgattag atacctataa
    13921 acttggtgaa gttaataaaa gacatattaa tgagcttaat aaagtaataa gtgaagagat
    13981 aagagcacag ctaggcatta aaaataaaaa ggaattgcaa acgaaaataa aacaaatatt
    14041 tactgattat ttaaataata agaattgggg accggtcgac aagaatatta gtcatcatgg
    14101 gaaaaattat ggttttcaat taacacctgc ctctcatatg aagataggga ataaaaatat
    14161 atttgtcaaa gagtataatg gaaaaggaat ttgttgtgct tctacgagag agagtgagca
    14221 tatcgcgaat atgtggcttt caaaagtggt ggatgacgag gggaaggaaa ttttttcggg
    14281 tatcagacat ggagttatat cagcctatgg attgaaaaaa aactcatctg aaagagctgt
    14341 tgctgctcgt aataaagctg aggagttagt aagcgcagca ttatatagca gaccagagtt
    14401 attatcacag gctttgtctg gtaaaacagt agatttaaag attgtttcga cttctcttct
    14461 gacgccgacc agtttaaccg ggggagagga gagtatgtta aaagatcagg tgaacgcatt
    14521 aaaagggtta aactctaaaa ggggggagcc aacgaaatta ttaattcgga atagtgatgg
    14581 tcttctgaaa gaagtaagcg ttaatctaaa agtggtgaca tttaactttg gtgtaaatga
    14641 attagcgcta aaaatgggct taggctggag gaatgttgac aagcttaatg atgaatcaat
    14701 atgttctttg ttgggggata atttcctcaa aaatggtgtg attggaggtt gggctgcaga
    14761 agcaatagaa aaaaatccac catgtaagaa tgatgtgata tatctggcta accagataaa
    14821 agagattata aacaagaagt tacaaaaaaa tgataatgga gagccgtata aattgtcaca
    14881 aagaatgact ctattggctt atactatagg tgctgtacct tgctggaatt gtaagagtgg
    14941 gaaggacaga acaggtatgc aggatgctga aattaaaaga gaaataataa gaaaacatga
    15001 aacaggtcag ttttctcaat taaatagtaa attatcctca gaagagaaaa gattattttc
    15061 tactattcta atgaatagtg gtaatatgga aatccaagag atgaatactg gtgttcccgg
    15121 aaataaagtc atgaaaaaat tgccgctatc ttcgttagag ctatcttatt ctgaaagaat
    15181 aggggaccca aaaatatgga atatggtgaa agggtattcg tcatttgtat aagaggaata
    15241 tatggaagat ttagcagatg ttatttgccg agccttgggt atccctttaa ttgatatcga
    15301 tgatcaagca atcatgcttg atgatgatgt gctaatatat attgaaaaag aaggagattc
    15361 aataaattta ttatgccctt tttgtgcatt gcctgaaaat ataaatgatc ttatatatgc
    15421 gttgagccta aattactcag aaaagatatg cttagctact gatgatgaag gcggaaatct
    15481 aattgcgcga ttagatctta ccgggataaa tgaattcgag gatgtatatg ttaatacaga
    15541 atattatatt tcgcgtgtgc gttggctgaa ggatgaattt gcgcgaagaa tgaaggggta
    15601 ttaatgtccc gtttcgtatt tattttgctg tgttttatcc catatttagg gagagctgat
    15661 tgttgggata aggctggtga aaggtacaat attccatcaa gtttgttaaa agcgattgcg
    15721 gaaaaagaat ccggatttaa taaatctgca gtcaatgtta ataacaatgg aagtaaagat
    15781 tatggtataa tgcaaataaa tgactttcat tctaaaagac ttagagaaat gggatattct
    15841 gaggaaatgc taattagtca tccatgtctt tctgtacatt atgcagcaaa gttattaaat
    15901 gagtttatga tgatgtatgg aagagggtgg gaggctgttg gagcctataa tgcaggaacg
    15961 tctccgaaaa agaaaaaaga aagattaaag tacgcagagg atatctatag acgctatttg
    16021 cggatagcag cagagagcaa gcagaataat cgaaggatat aagaggattt atgtctgagg
    16081 caaagaactc aaatcttgca ccattcaggt tattagtgaa actcacaaac ggtgtcgggg
    16141 atgagtttcc gttatattat gggaataact taattgttct cggacggacg atagaaacac
    16201 ttgagtttgg aaacgacaat ttcccagaaa atattattcc tgtaacagat agtaaatcgg
    16261 atggtataat ttatttgacc ataagcaaag ataatatctg tcaatttagt gatgaaaaag
    16321 gtgagcaaat tgatatcaat tcacaattta attcatttga atatgacggg atttcttttc
    16381 accttaaaaa tatgagggag gataaatcga gaggccatat actaaacgga atgtataaga
    16441 accactcggt gttttttttc tttgctgtta ttgttgtgtt aattataatt ttttccctct
    16501 ctttaaagaa agatgaagta aaagaaattg ctgaaattat agatgataag agatatggaa
    16561 tagtcaacac tggacagtgc aattatattc ttgccgaaac acaaaatgat gcggtatggg
    16621 cgtctgtagc cttaaataaa accggattta ccaaatgcag atatattctg gtgtcaaaca
    16681 aagaaataaa tagaattcaa caatatatca atcaacgatt cccatttatc aatttgtatg
    16741 tgttgaattt agtgagtgat aaagcggaat tactggtgtt tttaagtaaa gaaagaaata
    16801 gttcaaaaga taccgaactt gataaattga aaaatgctct gattgttgaa tttccgtata
    16861 tcaaaaatat taaattcaac tatcttagcg atcataatgc aaggggggat gctaaaggta
    16921 ttttcacaaa ggtaaatgtc cagtataaag agatttgtga aaataataaa gttacatatt
    16981 ctgtcagaga agaactgaca gatgaaaagc ttgagctcat taatagactg atttctgaac
    17041 ataaaaaaat atatggagat cagtatattg agttttctgt acttttgata gatgatgatt
    17101 ttaaaggtaa atcatatctt aacagcaaag acagttatgt aatgttgaat gataaacact
    17161 ggtttttttt tagataaaaa taagtgagga taaaatgagt gttacagtac cgaatgatga
    17221 ttggacattg agttcattat ctgaaacttt tgatgatgga actcaaacat tacaaggtga
    17281 actaacattg gcactagata aattagctag aaatccttcg aatccacagt tgctggctga
    17341 ataccaaagt aaattatctg aatatacatt atataggaac gcgcaatcca atacagtgaa
    17401 agtgattaag gatgttgatg ctgcaattat tcaaaacttc agataatagg gagcattcat
    17461 gaattacatt tatccagtca atcaggttga tattatcaaa gccagtgatt ttcaatctca
    17521 agagatatca agtctggaag acgtcgtgtc ggctaaatat agtgatatta agatggatac
    17581 agatattcaa gtatcacaaa taatggagat ggtaagcaat ccagaatcat taaacccaga
    17641 atctttggcc aagttacaga cgacgctctc aaattattca ataggagtat cattagctgg
    17701 cacgttagca agaaaaacag tttcggctgt tgaaacttta ttaaagtctt aatttatatg
    17761 attaggtata aaggttttat tttattcttg ttgctgatgt tgattggatg tgagcaacgt
    17821 gaagagttaa tttctaattt atctcaaaga caggcaaatg aaataatatc tgtgctagaa
    17881 cgccataata ttactgctag aaaagttgat ggaggtaaac aggggatctc ggtacaagtc
    17941 gaaaagggga catttgcatc ggcagttgat ttgatgcgca tgtacgattt gccaaatccg
    18001 gagagagttg atatctcaca aatgtttcct acagattcat tagtgtcttc tccaagagct
    18061 gaaaaggccc gtttatatag tgctattgag caacggctgg aacagtcttt agtttctatt
    18121 ggtggtgtta tttcggcaaa aatacatgtt agctatgatc ttgaagaaaa aaatatatct
    18181 tcaaaaccga tgcatatatc agtaatcgct atatatgact caccgaaaga gtctgaacta
    18241 ttagttagta atattaagcg atttttgaaa aacacctttt ctgatgttaa gtatgaaaat
    18301 atatctgtca tattaactcc gaaagaagaa tatgtttata caaatgtaca acctgttaag
    18361 gaagttaaat cggaattttt aacaaatgaa gtaatatatt tatttctcgg gatggctgta
    18421 ctagttgtca ttcttttggt atgggcattc aaaacagggt ggttcaagag aaacaaaata
    18481 tgataagaat ggatggaatt tataaaaaat atctttcaat aatttttgat ccagcgttct
    18541 atataaatag aaatcggttg aatttgcctt ctgaactgtt agaaaatggc gtaatcagaa
    18601 gtgagattaa taatctcata attaataaat atgatctaaa ttgcgatatt gaacctttaa
    18661 gcggggtaac cgctatgttt gttgccaact ggaatttact tccagctgtt gcttatttta
    18721 tcggtagtca agagtctcgg ttaattaatc atagtgagat ggttattagt tattatgggg
    18781 gcaaaatcag taagcaaggg gaggccgcta ttcgaagtgg tttttggcat cttattgcgt
    18841 ggaaggaaaa tatatctgtt ggtatatatg aaagaataaa cttattattt aatccaattg
    18901 ctttggaggg aaattatacg cctgtagaaa gaaatttgtc ccgattaaat gaaggtatgc
    18961 aatatgcaaa aaggcacttt accggtatcc agacatcatg cctatgatgg tgttgttata
    19021 aagagaattg aaaaggaatt atgtaaaacc ataaaagata gagacactga gagtaagaag
    19081 aaagcaattt gtgttataaa ggaggctacg aaaaaagctg aatcactgcg tatagatgct
    19141 gtatgtgatg gttatcaaat tggtatacag actgcttttg agcatattat tgactatatt
    19201 tgtgaatgga agttaaagca aaatgaaaac agaagaaata ttgaggacta tataactagt
    19261 ttgctttcag aaaatttaca tgatgagagg atcattagca ctttattgga gcagtggctt
    19321 agttcgttgc gtaatacagt aacagaatta aaagtggttt tgcccaagtg taatttagca
    19381 ctgagtaaaa agttagaact tgatctccat aaatatcgct cagatgtcaa gattatttta
    19441 aaatattctg aagggaataa ttatatattc tgtagtggta atcaagtagt ggagtttagt
    19501 cctcaggatg taatatcagg tgttaaaata gaacttgcgg aaaaattaac aaaaaatgat
    19561 aaaaaatatt tcaaagagtt agcacataag aaactgagac aaattgcaga agacttgtta
    19621 aaagagaatc ctgttaatga ttaatcaaat aaatgcaagc aatgctctgc agcaaagatt
    19681 aaatagtgaa gaatttgtta atttaaatga gagattatca tcttcgcaat catttgatga
    19741 ggatataatc tatgaaataa tgcaatattt ctcacagagt gaattaaatt ccatagataa
    19801 tgatgaactg cacaataaga ttgaacaatt attcaattca cgttttcctt atcttacagc
    19861 tgcacaaaaa agttcacttc ttaataaact catagatgca aatcaatatg tcgatttgca
    19921 tgagggattt tatgcatcat tgtccatata taacaatatt gatttctata ttaagactac
    19981 aaccttcgat tcattaatta gtgtctttga agcagggaga gaggcagatg attcgacatg
    20041 gtagtaataa gttgaaaata tttattttaa gtatattgct attaacactg agtgggtgtg
    20101 ctttaaagtc atcatctaat tctgaaaaag aatggcatat tgttcctgta agtaaggatt
    20161 atttttctat tccaaatgat ttattatggt cgtttaatac aaccaataaa agtataaatg
    20221 tttactctaa atgtattagt ggtaaggcgg tttatagttt taatgcaggt aaattcatgg
    20281 gcaactttaa tgttaaggaa gtagatgggt gcttcatgga tgcacaaaag atagctatag
    20341 ataaactatt ttctatgctg aaagacgggg ttgttttaaa aggtaataag ataaatgata
    20401 ccatccttat agagaaggat ggggaagtta aattaaaatt aattcgaggg atataattgt
    20461 attgtgagta aatataaagg tctaaataca agtaatatgt tttacattta ctctagtgga
    20521 catgaaccag ttaacgttga gcttgtaaaa gataaagaac gtaacataat tgagctggct
    20581 ccagcatgga agggtttttt tttgtccgaa atcaaaatat aaaattcagt gataatgtga
    20641 actatcatta tcgctttaac attaatagtt gtgcaaaatt tcttgctttt tgggactatt
    20701 tcagtggtgc tctagtagag catagccatg ctgaaaaatg tattcatttc tatcatgaga
    20761 atgatcttcg ggatagttgt aataccgaat cgatgcttga taagttaatg ttaagattca
    20821 tcttttcatc tgaccagaat gtaagtaatg cactggctat gatacgaatg actgagtcat
    20881 atcatttggt tctttatctt ctgagaacta ttgagaaaga aaaggaggta agaataaaga
    20941 gtctgacaga gcattatggt gtgtcggagg cttattttag atctttatgt agaaaagctt
    21001 taggggcaaa agtcaaagaa caactgaaca cttggcggtt agtaaatggt ttattagatg
    21061 tatttttgca caatcaaaca atcacttctg ctgccatgaa taatgggtat gcatcaacat
    21121 cccatttttc aaatgagata aaaacacgac taggcttttc tgcaagagaa ttatcgaata
    21181 ttactttttt agtgaaaaaa ataaatgaaa aaatttaata ttaaatcttt gactctcttg
    21241 attgtattgt taccattgat agttaatgct aataatattg actctcattt attagaacaa
    21301 aatgacatag cgaaatatgt tgctcaaagt gatacggtag gatctttttt tgaacgtttt
    21361 tctgcgttat taaattatcc tattgttgtt agcaaacaag ctgcaaaaaa gagaatatct
    21421 ggggagtttg atttatctaa tccagaagaa atgcttgaaa aactgacatt acttgtggga
    21481 ctcatatggt ataaagatgg aaatgcatta tacatatatg actctggaga attaataagc
    21541 aaagttattt tgctggagaa tatatcactt aattatctta ttcagtatct gaaggatgct
    21601 aacctttatg atcacagata tcctataaga ggaaacatta gtgataaaac attctatata
    21661 tctggtcctc cggcattggt tgaactggta gctaatacag caactttgct tgataagcag
    21721 gtttcttcta ttgggacaga taaagttaat tttggggtta taaaattaaa aaatacattt
    21781 gttagtgaca gaacttataa tatgagaggg gaggatattg tcatcccagg ggtggctact
    21841 gttgtagaac gtttattaaa taatggtaag gctctgagta acagacaagc ccaaaatgat
    21901 ccgatgccac cctttaatat tacacagaaa gtatctgagg atagtaatga cttttcattt
    21961 agttcagtta ccaatagttc tatattagag gatgtatcac ttattgcata tccagaaacc
    22021 aattctatat tagtcaaagg taatgatcag caaatacaga ttattcgcga tattatcact
    22081 cagttagatg tagccaaacg acatatagaa ttatcattat ggataatcga tattgataaa
    22141 tctgaattaa ataaccttgg tgttaattgg caaggaacgg cttcatttgg tgattcgttt
    22201 ggtgcgtcat ttaatatgag ctcatcagca tcgataagca ctttggacgg caataagttt
    22261 attgcttcag ttatggcatt aaatcagaaa aagaaagcaa atgtagtttc aaggccggtg
    22321 attttgacac aagagaatat tccggctata tttgataata acaggacttt ttatgtgtca
    22381 ttagttggtg agcgcaattc ttcgttggag catgttacgt atggaacatt gataaatgtt
    22441 atacctcgct tttcatctcg gggacaaatt gagatgtctt taacgataga ggatggaaca
    22501 ggaaatagtc agtcaaatta caattataat aatgaaaata catctgtcct tccagaagtt
    22561 ggaaggacca aaatcagtac aatagcgaga gttccacaag gcaaaagttt gttgataggt
    22621 ggttatacgc atgaaacgaa ctctaacgaa atagtatcta ttccttttct ctcctctatt
    22681 cctgtaatcg gtaatgtgtt caagtataaa acaagtaata tcagtaatat tgtaagggtt
    22741 tttctgattc aaccccgtga gataaaggag tcaagttatt ataatactgc agaatataaa
    22801 agcctcatat ctgaaagaga aatacaaaag actacacaaa taatcccatc tgaaactact
    22861 ttgctggaag acgaaaaatc attggtttca tacttaaatt actaactata aagtaggtga
    22921 tgtatgcttg atgttaaaaa tacaggagtt tttagctctg cattcattga taagttgaat
    22981 gcaatgacaa attcagatga tggagatgag actgctgatg cagagcttga ttctggcttg
    23041 gctaatagca agtatattga ctcatctgat gagatggctt ccgctctttc gtcatttata
    23101 aacagaagag accttgagaa actgaaagga acaaatagtg atagtcagga acgtatttta
    23161 gatggggaag aagatgaaat taatcacaag atttttgatt taaagagaac gttaaaagat
    23221 aaccttcctc tagatcggga ttttatagac agactaaaga gatattttaa agatccaagt
    23281 gatcaagtct tagcattaag ggaacttttg aatgaaaaag atcttactgc tgaacaagtc
    23341 gaattattaa ctaaaattat taatgagata atatcaggta gtgaaaaaag tgttaatgct
    23401 ggaataaatt cagctataca ggctaaatta tttggcaaca aaatgaaact tgaaccacag
    23461 cttttgcgtg catgttatcg tggttttatc atggggaaca tatcaacaac agatcagtat
    23521 atagaatggc ttggtaattt tggttttaat cacagacata caattgtgaa ttttgtagag
    23581 cagtcactga ttgtagacat ggattctgag aaaccgagct gtaatgctta tgagtttggt
    23641 tttgtgttat ctaaattaat tgcaattaag atgattagaa cttcagacgt aatttttatg
    23701 aagaaactgg aatcctcaag cttgctaaaa gatggcagtt taagtgcaga gcagctattg
    23761 ctaactttat tatatatttt tcaatatcca agtgaaagtg agcaaattct tacttctgtt
    23821 atagaagtat cacaagccag tcatgaggat tctgtagtgt atcaaacata tctatcttct
    23881 gttaatgaaa gtcctcatga tatatttaaa agtgaaagtg aaagagaaat tgcgatcaat
    23941 attctacgag agcttgtcac aagtgcatac aagaaagagc tttctagata acaggagata
    24001 aaagtgatcc agtcttttct taagcaagta agtactaagc ctgaattaat tatattagtg
    24061 cttatggtta tgatcattgc tatgttgatc ataccattac ctacatacct tgttgatttt
    24121 ttaatagggc ttaatattgt acttgctatt ctcgttttta tgggatcatt ttatattgaa
    24181 agaatattga gtttttctac attcccttcg gttttgttga taacgacatt atttcgtctc
    24241 gctttatcaa taagtactag tcgactaatt ttagttgatg cggatgcggg taaaattatt
    24301 actacatttg gccagtttgt cattggtgat agtctggctg taggttttgt tattttttca
    24361 atagtaactg ttgtgcagtt cattgttata acaaaaggat ctgaaagggt tgcggaggta
    24421 gctgctcgct tttctcttga cggtatgccg gggaaacaaa tgagcattga tgcggattta
    24481 aaggctggaa ttattgatgc cgcaggagct aaagaaagac gcagtattct ggaacgtgag
    24541 agtcaattgt atggttcatt tgatggagca atgaagttca ttaaaggtga tgcaattgct
    24601 ggcatcatta ttatttttgt caatttaata ggaggtattt ctgttggtat gagtcaacat
    24661 ggaatgtccc tatctggtgc attatctact tataccatac taactattgg tgacggattg
    24721 gtctctcaga ttcctgctct gttaatttct ataagtgcag gatttatcgt tacaagagtt
    24781 aatggtgata gcgataatat gggacgtaac ataatgtccc agatatttgg gaatcctttt
    24841 gttctcattg ttacatcagc tcttgccttg gcgataggga tgttgccagg ttttccgttt
    24901 tttgtttttt tcctgatagc agttactttg acggctttat tctattataa aaaggtcgta
    24961 gaaaaagaaa aaagtctgtc tgagtctgat tctagtggct atactggtac atttgatatt
    25021 gataatagcc atgactcatc tttggcaatg atagaaaatc tggatgctat tagttcagaa
    25081 accgttcctt taattttatt atttgccgaa aataagataa atgcaaatga tatggaaggt
    25141 cttattgaaa ggataagaag tcagttcttt attgattatg gtgtcaggct tccaactatt
    25201 ttatatagaa caagcaatga gcttaaggtc gatgatattg ttttgttaat aaatgaggtg
    25261 cgtgccgata gttttaatat atattttgac aaagtgtgta ttacagatga aaatggagat
    25321 atagatgctc taggtattcc tgttgtttca acttcatata atgaacgtgt tatttcttgg
    25381 gtagacgttt catatacaga aaatctaact aatattgatg ctaaaattaa aagtgctcaa
    25441 gatgaatttt atcaccagtt gtcacaagct ttattaaaca acataaatga gatttttggt
    25501 atacaagaaa caaaaaatat gttagatcag tttgaaaatc ggtatcctga tctattaaag
    25561 gaagtcttcc gacatgtgac tatacagaga atttctgagg tattacaaag attgcttgga
    25621 gaaaatattt ctgttcgcaa tttaaaactt attatggagt ctttggcgct ttgggctccc
    25681 agagaaaaag atgtcataac attagttgaa catgtccgtg catcactttc taggtatatt
    25741 tgtagtaaaa tagctgtttc tggtgagatt aaagttgtga tgctttccgg atatattgag
    25801 gatgcaataa gaaaagggat aaggcaaacc tctggtggct ctttcttgaa tatggatata
    25861 gaggtttcgg atgaggtaat ggaaacttta gcacatgctt tgagagaatt gagaaatgca
    25921 aaaaaaaatt tcgttctttt ggtatcagta gatatacgta ggtttgttaa aagacttata
    25981 gataacagat ttaagagtat actcgttata tcgtatgctg agattgatga agcatatacc
    26041 attaatgtat taaagactat ttagtgaggt ttaaatatga gtaacattaa tttagttcaa
    26101 ttagttagag atagtctttt cacgattggt tgtcctccat caattattac agatttagac
    26161 agccattcag cgattaccat ttctttggat agtatgcctg ctattaatat agctcttgtc
    26221 aatgagcagg ttatgttatg ggctaatttt gatgcaccaa gtgatgtaaa acttcagtca
    26281 tctgcttata acatattaaa tttaatgtta atgaacttta gttatagcat taatgagctg
    26341 gtagagcttc atcgttctga tgagtactta cagttaagag ttgttattaa agatgactac
    26401 gttcacgatg ggattgtttt tgcagagatt ctgcatgagt tttatcagag gatggaaatc
    26461 ttaaatgagg tcttataatc aatgagctat acaaaattgc tcactcaatt atcttttcct
    26521 aatagaatct cggggccaat cttggaaaca agtcttagcg atgtttcgat tggtgagatt
    26581 tgtaacattc aggctggaat tgaaagtaat gaaattgttg caagagctca ggttgtagga
    26641 tttcatgatg aaaaaacaat attaagcttg attggaaatt ctcgtggact ttcacggcaa
    26701 acgttgatta agcccactgc ccagtttctt catacgcaag tcggccgtgg attattggga
    26761 gcagtagtca atcctttagg ggaggttact gataagtttg ctgttacgga taacagtgaa
    26821 attctttatc gacctgtaga taatgctcct ccgctatata gtgaaagggc tgcaattgag
    26881 aagccttttt taacaggtat taaggttatt gattctttac tcacgtgtgg tgaaggacag
    26941 cgaatgggga tttttgcgtc agctggttgt ggcaaaactt ttctcatgaa tatgctcatt
    27001 gaacatagtg gtgctgatat atatgttatt gggttaattg gtgagcgagg tcgagaggtt
    27061 acagaaacgg ttgattattt gaaaaactct gagaaaaaaa gcaggtgtgt tttagtatat
    27121 gcaacttcgg attactcttc ggttgatcgt tgtaatgctg catatatagc cactgctata
    27181 gccgaatttt ttaggactga aggacataaa gtagcgcttt ttattgattc attaacaagg
    27241 tatgccagag cattacgtga tgtggcctta gccgctggag aatcacctgc cagaagaggc
    27301 tatccggttt cggtttttga tagcttaccc agacttcttg aaaggccagg aaagttaaag
    27361 gcaggtggct ctattactgc attttacact gttcttttgg aggatgatga ttttgctgat
    27421 ccattagctg aagaggtaag atccatttta gatggacata tatatttgag cagaaatcta
    27481 gcccaaaaag gacaatttcc tgcaattgat tccttaaaaa gtataagcag ggtatttaca
    27541 caggttgttg atgaaaaaca tcgtattatg gccgctgcat ttcgggagct actttctgaa
    27601 atagaagagc taaggacaat tattgacttt ggtgaataca aaccggggga gaatgcctct
    27661 caggataaaa tctacaacaa aatatctgtt gttgagagtt ttctgaagca agattatcga
    27721 ctcggtttta cttatgagca gacaatggag cttattggtg aaacaattag ataaggtttt
    27781 aaaaatcaaa gataaatatc aacggtctgt taaattaatt gaagcacaca tattaacact
    27841 aaaaaaaaaa ctctttgtat cgagatgtgg aggcattaga taaaaggatt atatacttcc
    27901 tgcaactgga gaatgactta gaacctgtag gcgctcagag cgttagtcag cttttcaata
    27961 caagacgtaa gatcgctata gtaaaaaagc atattatcca atatcagtct gaacgaattc
    28021 ttctaaaggg aagaattgaa gaaattcaaa aagatattga tgaggcaaat gcttctaaaa
    28081 gaaaactgct tcataaagag agtaaaatat gtaaacggat tggcttgatt aaaagaaata
    28141 actttgccaa acagttgata cttgatgagt tatcacaaga ggatatgaag tatggcatta
    28201 gataatataa acctaaattt tagtagtgac aaacagatag aaaaatgtga gaaactatct
    28261 tcaatagata atattgatag tctcgttttg aagaagaaga ggaaggttga aattcctgag
    28321 tactctttaa ttgcatctaa ctattttact attgataagc actttgaaca taagcatgat
    28381 aaaggagaaa tttatagtgg cattaaaaat gcgttcgaac ttagaaacga acgagcgaca
    28441 tattctgata ttccagaatc aatggccatt aaagaaaata ttttgatacc agatcaagat
    28501 atcaaagcaa gggaaaaaat aaatatcggc gatatgaggg ggatcttttc atataataag
    28561 agtggaaatg cagacaagaa cttcgaaaga agtcatactt cttctgtaaa ccctgataat
    28621 ctgctagaat ctgataatag aaatggtcaa attggtttaa aaaatcatag cttgtctatt
    28681 gataagaata ttgctgacat catttcttta ctaaatggaa gtgttgctaa atcatttgag
    28741 ctgcctgtaa tgaataaaaa tactgcagac ataaccccat ccatgtcatt gcaagaaaaa
    28801 tcaatagttg aaaatgataa aaatgttttt caaaaaaata gtgaaatgac ttaccacttt
    28861 aaacagtggg gggctggaca ttctgttagt atttcagtgg agtctggttc ttttgttcta
    28921 aaaccgtcag atcaatttgt aggaaataaa cttgacttaa ttttgaaaca agatgctgag
    28981 ggtaattaca gatttgatag cagtcaacat aataagggga ataaaaataa tagtacagga
    29041 tataatgaac agagtgaaga agaatgctaa gaattaaaca ttttgacgct aacgaaaaac
    29101 tacagatttt atatgcaaag caactctgcg agcgtttttc gattcagaca ttcaaaaata
    29161 aatttacagg cagtgaaagt ttagtcactc ttacttccgt gtgtggggat tgggtaattc
    29221 gtattgatac attatctttt ttgaaaaaaa aatacgaggt attttcagga ttttctacac
    29281 aagaatcttt actgcattta tcaaaatgtg tctttataga gtcgtcatct gtattttcga
    29341 ttccagaact gtctgataag attactttcc ggatcacgaa tgaaatccag tatgcaacta
    29401 ctgggagtca tttatgctgt ttttcctctt ctttaggtat tatttatttt gacaagatgc
    29461 cggtattacg taatcaagtt tctcttgact tattgcatca tcttttagag ttttgcttag
    29521 gttcatctaa tgtaaggctg gctactttaa aaagaattcg cactggtgat ataatcatag
    29581 ttcagaaact ttataattta ttattgtgta atcaagttat tattggggat tatattgtga
    29641 atgataataa tgaggcaaaa attaatctgt cagaaagtaa tggtgagtca gaacacacag
    29701 aagtttcttt ggcattattc aattatgatg atatcaatgt aaaagtggac tttattcttt
    29761 tagaaaaaaa tatgacaatc aatgaactaa aaatgtatgt agaaaacgaa ttattcaagt
    29821 ttcccgatga catagttaaa catgtaaata ttaaagtaaa tggttctttg gttgggcatg
    29881 gggaacttgt ttctattgag gatggttatg gtatcgagat tagttcttgg atggtaaagg
    29941 agtaatctca tgctgagtga catgtccctc atagctacac tttctttttt tacgctatta
    30001 ccatttttag ttgctgctgg tacctgttat ataaagtttt ctattgtttt tgtaatggta
    30061 cgaaatgcac ttggtttgca gcaggttcca tcaaatatga ctctcaatgg tattgcattg
    30121 attatggctt tgttcgtgat gaaacccatt attgaagctg gttatgaaaa ttatctaaat
    30181 ggccctcaaa aatttgatac aatatctgat attgtccgtt tttctgattc tggcttgatg
    30241 gaatataagc aatacttgaa aaagcataca gatcttgagt tagcacgttt tttccaaagg
    30301 tctgaagaag aaaatgctga tttaaagagt gcagagaata atgattattc attgttttcc
    30361 ctcttgcctg catatgcgct aagtgaaatc aaagatgcgt tcaaaatagg tttttattta
    30421 tatttgccat ttgttgttgt tgatcttgtt atttcaagca ttttacttgc acttggtatg
    30481 atgatgatga gtccaatcac aatatcagta ccaataaaac ttgtattatt tgttgcgctt
    30541 gatgggtggg gtatcctgtc taaggccttg attgaacaat atatcaatat tcctgcttag
    30601 atgatggagg taatgtgtct gatatagttt atatgggtaa taaggctctt tatttaatcc
    30661 ttatcttttc cttatggcca gtaggtatag ctacggttat tggattatct attggtttgt
    30721 tacagacagt gactcaactc caagagcaga cacttccttt tggtataaag cttataggtg
    30781 tctcaatatc tttgctactt ctttctggat ggtatggtga ggttttattg tctttttgtc
    30841 atgaaataat gtttttaatt aagagtgggg tttgatggac atttcaagct ggttcgaaag
    30901 tattcatgtg tttttaatac tcctgaacgg cgtttttttt agattggctc cattattttt
    30961 ctttcttcca tttttaaata acggtataat ttctccatct attagaatac ctgtgatttt
    31021 tcttgttgca tcaggattaa ttacttctgg taaggtagac ataggttctt ctgtttttga
    31081 acatgtttat ttccttatgt tcaaggaaat aattgttggc ctccttctct ctttttgctt
    31141 gtctcttccc ttttggatat ttcatgctgt tggtagcatt attgacaacc agcgtggggc
    31201 aacgcttagt agttcaattg atcctgccaa tggtgttgat acgtctgagt tggcaaaatt
    31261 tttcaatctt ttttctgcag ttgtatttct atacagtggt ggtatggtct ttattttaga
    31321 atccatacaa ttgtcttata atatatgccc gttattttct caatgttctt tccgtgtctc
    31381 aaatatctta acatttctga ctttattggc aagtcaggct gttattttag ccagtcctgt
    31441 tatgatagta ttgttactat cagaagtatt acttggtgta ttatcgagat ttgctccgca
    31501 gatgaatgct ttttccgtat cattaactat taaaagttta cttgcaatat ttattatttt
    31561 catctgttct tctactattt acttttctaa agttcaattt ttcctcggtg aacataaatt
    31621 tttcacaaat ctatttgtta gataaaatat tatggcaaat aaaacagaaa agccgacacc
    31681 taaaaaacta aaggatgccg caaaaaaagg acagtcattt aaatttaagg atttaacgac
    31741 tgttgttatt attctggtag ggacatttac tataatatca ttcttttcct taagtgatgt
    31801 aatgctctta tacagatatg taataattaa tgacttcgaa attaatgagg gtaaatactt
    31861 ttttgcagtg gttattgtct tttttaagat aattggcttc ccactttttt tctgtgttct
    31921 ttcggctgtg ttgccaacat tggttcaaac aaagtttgtt cttgcgacta aagctatcaa
    31981 gattgatttt tcagtattaa accctgttaa agggttaaaa aaaatattta gtataaagac
    32041 aataaaagaa tttttcaaaa gcattctgct tcttattatt ctagcattaa caacctattt
    32101 cttttggatt aatgaccgaa aaataatttt ttctcaggtg ttttctagtg ttgatggctt
    32161 atatcttatt tggggggggc tgtttaagga tataatatta tttttcttgg cattttctat
    32221 ttttgttatt attcttgact ttgtgattga gttcatttta tacatgaaag atatgatgat
    32281 ggataaacag gagataaaaa gagaatatat agagcaagag ggacactttg agacaaagtc
    32341 gagaaggcgt gagttgcata tcgagattct ttcagagcag actaaatctg atatacgtaa
    32401 ttcaaaatta gtggtaatga acccgactca tattgcaatt ggtatttatt ttaatccaga
    32461 aatagcgcct gcacctttta tttctctcat tgaaactaac cagtgtgcct tggctgtcag
    32521 aaaatatgca aatgaagttg gtataccgac tgtgcgtgat gtgaaattag ctagaaaact
    32581 atataaaaca catacaaaat atagttttgt tgattttgaa cacttggatg aagtcctacg
    32641 tcttattgtt tggcttgagc aggttgaaaa cactcattaa taagtaaagg agatgtgtta
    32701 tatgggagtt aatttctgta ataaaatagg tattgatcag tctgaatttg aaatagaatc
    32761 ttccatcatt aactccattg ctaatgaggt attgaaccca atatctttcc ttagcaataa
    32821 ggatataata aatgttttac tcagaaagat ttcatctgaa tgtgacctgg ttagaaaaga
    32881 catttatcgt tgtgctctgg agttggtcgt tgaaaaaaca cctgatgatt tataatcatt
    32941 cataataact cttttgctga tataaatatt tttgttgaag atatttatgt agtcacagga
    33001 tcaattgtag aaaattgtta tgtttttgtt aggaactgca ctaaataata tatgataaga
    33061 cagcaaaaac gactgaccat aatccttctt ttattaggag ttgataagag agactattca
    33121 tcctgtaatg ttaaaactct gttatatagt attcgcgatt atgcaaagtc tgtaaatgat
    33181 catgagatac taaccgagag taatcgttta ttatctcatt gcatttcaga tagtaacgga
    33241 gctttcttta aaagtagtaa atacgttccc cttaagtact tacgtaaaag gagaatagca
    33301 cgaaagatac caaatgatta gcactttgct agtaagcttg ctggtaaact atattgaccg
    33361 ttgggtttgt tttgagactg taatttaaat tgtgtaattg cctgtttttg atatgtttac
    33421 tccaacaacg gagacaggta aattatggac gaaaagaagc ttaaagccct tgcggctgaa
    33481 ctggctaaag gcctcaaaac tgaggccgac ctcaatcagt tttcccgtat gctgacgaaa
    33541 tatcccggct cttaaaacga tagtggctca ggctttcatt tttcagcgtt ccccagaag
//
LOCUS       DAJTYG010000038        33046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTYG010000038 DAJTYG010000000
VERSION     DAJTYG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.038"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            16..4593
                     /locus_tag="OO568_001916"
     CDS             16..4593
                     /locus_tag="OO568_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCR6641280.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRSSVASG
                     TDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNT
                     IALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLA
                     LGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQ
                     LYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTK
                     GKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTTNITNLTDS
                     VGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAV
                     ATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT
                     NVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKW
                     DKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVN
                     TDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHGTNGDA
                     SVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANAD
                     YDNVGDALNAIDTTPDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSD
                     AINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALL
                     WDKTANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQII
                     NQLAGNTDATYIEENGAGINYVRTNDNDLAFNDASASGVGATAVGYNAVASGASSVAI
                     GQNSSSTVDTGIALGSSSVSSRVIAKGSRDTSVTENGLVIGYDTTDGELLGALSIGDD
                     GKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAM
                     GAKTVVNGNAGIGIGLNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGY
                     TAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSI
                     TNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIA
                     AADNSVALGTGSVAEEENTISVGSSTNQRRITNVAASVNATDAVNVSQLKSSEAGGVR
                     YDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSAQETKQYTDQR
                     MVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSM
                     VSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            4961..6616
                     /gene="lldP"
                     /locus_tag="OO568_001917"
     CDS             4961..6616
                     /gene="lldP"
                     /locus_tag="OO568_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6641281.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDITLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6616..7392
                     /gene="lldR"
                     /locus_tag="OO568_001918"
     CDS             6616..7392
                     /gene="lldR"
                     /locus_tag="OO568_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6641282.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7389..8579
                     /gene="lldD"
                     /locus_tag="OO568_001919"
     CDS             7389..8579
                     /gene="lldD"
                     /locus_tag="OO568_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6641283.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            8775..9248
                     /gene="trmL"
                     /locus_tag="OO568_001920"
     CDS             8775..9248
                     /gene="trmL"
                     /locus_tag="OO568_001920"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6641284.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9301..10122)
                     /gene="cysE"
                     /locus_tag="OO568_001921"
     CDS             complement(9301..10122)
                     /gene="cysE"
                     /locus_tag="OO568_001921"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6641285.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10202..11221)
                     /gene="gpsA"
                     /locus_tag="OO568_001922"
     CDS             complement(10202..11221)
                     /gene="gpsA"
                     /locus_tag="OO568_001922"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6641286.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11221..11688)
                     /gene="secB"
                     /locus_tag="OO568_001923"
     CDS             complement(11221..11688)
                     /gene="secB"
                     /locus_tag="OO568_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6641287.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11751..12002)
                     /gene="grxC"
                     /locus_tag="OO568_001924"
     CDS             complement(11751..12002)
                     /gene="grxC"
                     /locus_tag="OO568_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6641288.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12144..12575)
                     /locus_tag="OO568_001925"
     CDS             complement(12144..12575)
                     /locus_tag="OO568_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6641289.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12820..14364
                     /gene="gpmM"
                     /locus_tag="OO568_001926"
                     /gene_synonym="pgmI"
     CDS             12820..14364
                     /gene="gpmM"
                     /locus_tag="OO568_001926"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6641290.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPC
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14374..15657
                     /gene="envC"
                     /locus_tag="OO568_001927"
     CDS             14374..15657
                     /gene="envC"
                     /locus_tag="OO568_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6641291.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKEHAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15661..16620
                     /locus_tag="OO568_001928"
     CDS             15661..16620
                     /locus_tag="OO568_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6641292.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQHEAAKFNASAN"
     gene            complement(16607..17641)
                     /gene="waaH"
                     /locus_tag="OO568_001929"
     CDS             complement(16607..17641)
                     /gene="waaH"
                     /locus_tag="OO568_001929"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCR6641293.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17880..18905)
                     /gene="tdh"
                     /locus_tag="OO568_001930"
     CDS             complement(17880..18905)
                     /gene="tdh"
                     /locus_tag="OO568_001930"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6641294.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18915..20111)
                     /gene="kbl"
                     /locus_tag="OO568_001931"
     CDS             complement(18915..20111)
                     /gene="kbl"
                     /locus_tag="OO568_001931"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6641295.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEDRLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(20386..21243)
                     /gene="yibB"
                     /locus_tag="OO568_001932"
     CDS             complement(20386..21243)
                     /gene="yibB"
                     /locus_tag="OO568_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR6641296.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDLKKKFRYIRNRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            21548..22480
                     /gene="rfaD"
                     /locus_tag="OO568_001933"
     CDS             21548..22480
                     /gene="rfaD"
                     /locus_tag="OO568_001933"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6641297.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22490..23536
                     /gene="rfaF"
                     /locus_tag="OO568_001934"
     CDS             22490..23536
                     /gene="rfaF"
                     /locus_tag="OO568_001934"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6641298.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23540..24532
                     /gene="rfaC"
                     /locus_tag="OO568_001935"
     CDS             23540..24532
                     /gene="rfaC"
                     /locus_tag="OO568_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6641299.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLDYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            24529..25737
                     /gene="rfaL"
                     /locus_tag="OO568_001936"
     CDS             24529..25737
                     /gene="rfaL"
                     /locus_tag="OO568_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR6641300.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(25773..26915)
                     /locus_tag="OO568_001937"
     CDS             complement(25773..26915)
                     /locus_tag="OO568_001937"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR6641301.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(26924..27937)
                     /locus_tag="OO568_001938"
     CDS             complement(26924..27937)
                     /locus_tag="OO568_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR6641302.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(27962..28669)
                     /gene="rfaY"
                     /locus_tag="OO568_001939"
     CDS             complement(27962..28669)
                     /gene="rfaY"
                     /locus_tag="OO568_001939"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6641303.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(28695..29702)
                     /gene="waaO"
                     /locus_tag="OO568_001940"
                     /gene_synonym="rfaI"
     CDS             complement(28695..29702)
                     /gene="waaO"
                     /locus_tag="OO568_001940"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6641304.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(29745..30542)
                     /gene="rfaP"
                     /locus_tag="OO568_001941"
     CDS             complement(29745..30542)
                     /gene="rfaP"
                     /locus_tag="OO568_001941"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6641305.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(30535..31659)
                     /locus_tag="OO568_001942"
     CDS             complement(30535..31659)
                     /locus_tag="OO568_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6641306.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(31656..32714)
                     /gene="rfaQ"
                     /locus_tag="OO568_001943"
     CDS             complement(31656..32714)
                     /gene="rfaQ"
                     /locus_tag="OO568_001943"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6641307.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         8735 a         8083 c         7799 g         8429 t
ORIGIN      
        1 tagcgagcat cattaatgaa caaaatattt aaagttatct ggaaccctgc gacagggaat
       61 tatactgtta ccagcgaaac ggcaaaaagc cgtggcaaga aatctgggcg cagtaagctg
      121 ttaatttctg cgctggttgc gggtggaatg ttgtcgtcgt ttggggcatt ggcgaatgcc
      181 gggaatgaca acggtcaggg tgttgattac ggtagtggat cagctggcga cggctgggtt
      241 gctataggca aaggggcgaa agcaaatact tttatgaaca ccagtggttc cagtactgct
      301 gtgggttatg acgctatagc tgaaggccaa tatagctctg ccatcgggtc aaaaacccat
      361 gcgattggtg gtgcatcaat ggcctttggg gttagtgcaa tatcagaagg cgatagaagt
      421 atagcactgg gtgcctcttc gtattcattg ggccaatact caatggccct ggggcgtagc
      481 agtgtagcta gcggtactga cagcctcgca tttggcagac aatcacttgc cagcgcagcg
      541 aacgctattg cgataggtgc tgagaccgaa gccgctgaaa atgcaactgc tattggcaat
      601 aatgcgaagg caaaagggac taatagcatg gcaatggggt tcggaagcct tgccgataaa
      661 gtcaatacta tcgcattagg aaatggcagc caggctctgg cagataatgc aatcgccata
      721 ggccagggca acaaagctga tggcgtggat gccatcgctc tgggtaatgg tagccagtcg
      781 agaggcttaa acaccattgc cttaggcaca gccagtaatg caactggtga taagagtctt
      841 gcgcttggta gtaatagcag tgccaacggt attaactctg tcgcgctggg cgcagattcc
      901 attgcggatt tagacaatac cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt
      961 aatgtgaaaa atggcgcgat caagtctgac agttacgatg ccattaatgg ttcacagctt
     1021 tatgccatta gcgactcggt agcaaaaagg cttggaggag gggctgcagt agatgttgat
     1081 gacggtactg ttacagcacc aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg
     1141 gctgcgctcg ctgtacttga tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac
     1201 agcgctgctc atggtactag tagcccaact gccagcgtaa tcaccgatgt tgcggatggc
     1261 acgatttcag cctccagtaa ggatgcggtt aacggttccc aactgaaagc taccaatgac
     1321 gatgtcgaag ccaacaccac caatatcacc aacctgacgg attccgttgg tgaccttcag
     1381 gctgatgccc tgctctggaa cgaaactaaa aaggcattca gtgcagctca cggccaggat
     1441 accaccagca aaataaccaa cgttaaagat gccgacctga cggctgacag cactgatgct
     1501 gttaacggct ctcagctgaa aaccaccaac gatgctgtgg cgacgaatac caccaatatc
     1561 gccaataaca cttccaatat tgccactaac accaccaaca tctctaacct gactgagacg
     1621 gtgactaatc ttggtgagga tgcgctgaaa tgggataagg acaatggtgt attcacggca
     1681 gctcatggca ccgagaccac cagcaaaata accaacgtta aagatggcga cctgacgact
     1741 ggcagcaccg atgccgttaa cggctctcag ctgaaaacca ccaacgatgc cgtggcgacg
     1801 aataccacca atatcgccac taacaccacc aacatctcta atctgactga gacggtgact
     1861 aatcttggtg aggatgcgct gaaatgggat aaggacaatg gtgtcttcac tgcagctcat
     1921 ggcaacaata ccgccagcaa aatcaccaat atcctggacg gcacagtcac tgcaaccagt
     1981 tccgatgcca ttaacggtag ccagctttat gacttaagca gcaatatcgc cacctacttc
     2041 ggcggcaatg cttctgtgaa tactgacggc gtgtttaccg gtccaaccta caaaatcggt
     2101 gaaacaaatt attataacgt cggcgatgca ctggctgcga ttaactcctc atttagcacg
     2161 tctctcggcg atgctctgct ttgggatgcc accgcaggta aattcagtgc caaacacggt
     2221 actaatggtg acgcaagcgt gatcactgat gtcgcagatg gtgaaatttc agactccagt
     2281 tctgacgcag taaacggctc acaactccac ggcgtgagca gttatgttgt tgatgcgctg
     2341 gggggtggtg ccgaagtcaa tgcagacggc accatcactg cgccgacgta caccattgct
     2401 aatgctgatt acgataatgt cggtgatgcc ctgaatgcta tcgataccac tcctgacgac
     2461 gctctgctct gggatgcgga cgccggtgaa aatggtgcat ttagcgccgc tcacggaaaa
     2521 gataaaactg ccagtgtaat cactaacgtc gctaacggtg caatctctgc tgccagcagc
     2581 gacgcgatta acggctcaca actctatacc accaataagt acatcgctga tgcgctgggt
     2641 ggtgacgcag aagtcaacgc tgacggcacc atcaccgcac cgacttacac cattgcgaac
     2701 gccgagtaca acaacgtcgg tgacgccctg gatgcgcttg atgataacgc cctgctgtgg
     2761 gataagactg ccaatggcgg tgctggagcc tacaatgcca gccatgacgg taaagccagc
     2821 atcatcacta atgtcgctaa tggcagtatt agtgaggaca gtaccgatgc agtgaacggt
     2881 tctcagttga atgcgacgaa tatgatgatt gagcagaaca cccaaattat caatcagctc
     2941 gctggtaaca ccgacgcaac ctatatcgaa gaaaatggcg ctggtatcaa ctacgttcgt
     3001 actaacgaca acgatttagc ctttaacgat gcaagcgcct ctggtgtcgg cgctacagct
     3061 gtaggttata acgctgtcgc gtctggtgcc agcagcgtag ccattggtca gaacagcagc
     3121 agcaccgttg ataccggtat tgcgctgggt agcagctccg tttccagccg tgtgattgcc
     3181 aaaggttctc gtgacactag cgtaacggaa aatggcttgg tgattggtta tgacaccact
     3241 gacggcgaac tgctaggtgc attgtcaatt ggtgatgacg gtaaataccg ccaaatcatc
     3301 aacgtagctg atggttcaga agcccatgac gccgttacgg ttcgccagtt gcagaacgct
     3361 attggagcgg tcgccactac gccaaccaag tactttcacg ccaactcaac ggcagaagac
     3421 tcactggccg ttggtgaaga ctcgctggca atgggtgcga aaactgtcgt taatggtaat
     3481 gcaggtattg gtatcggttt gaacactctg gttctggctg atgcgatcaa cggcattgct
     3541 atcggcagta acgcacgcgc aaaccatgca aacagtatcg caatgggtaa cggttctcag
     3601 accacccgtg gtgcacagac tggctacacc gcctacaaca tggacgcacc gcagaactct
     3661 gtaggtgagt tttctgtcgg tagtgaagac ggtcaacgtc agatcacaaa cgtcgcagct
     3721 ggttcagcgg ataccgatgc ggttaacgtg ggtcagttga aagtcactga tgagcgcgta
     3781 gcgcaaaata cccagagcat tactaacctg aacaatcagg tcactaatct ggatactcgc
     3841 gttactaata tcgaaaacgg tattggcgac attgtcacca ccggtagcac caagtacttc
     3901 aagaccaaca ccgatggcgt agatgccaac gcccagggta aagatagcgt tgctattggt
     3961 tctggttcca ttgctgccgc tgacaacagc gtcgcactgg gtaccggttc cgttgcagag
     4021 gaagaaaata caatctctgt aggttcttct actaaccaac gccgtatcac caacgtagct
     4081 gccagtgtta atgccaccga tgcggttaac gtgtcgcagc tgaaatcttc tgaagcaggc
     4141 ggagtgcgct acgacaccaa agctgatggt tctatcgact atagcaatat caccctcggt
     4201 ggtggcaacg gcagtacgac tcgtatcagc aacgtctccg ctggcgtcaa caacaacgac
     4261 gcggtgaact acgcgcagtt gaagcaaagc gcgcaggaaa cgaagcaata caccgatcag
     4321 cggatggttg agatggataa caaactgtct aaaactgaaa gcaagttgag cggtggtatc
     4381 gcttctgcaa tggcaatgac cggtctgccg caggcttata caccgggtgc cagcatggct
     4441 tctattggtg gcggtactta caacggtgaa tcggcagttg ctttaggtgt atcgatggtg
     4501 agcgccaatg gtcgttgggt ctacaaatta caaggtagta ccaatagcca gggtgaatac
     4561 tccgccgcac tcggtgccgg tattcagtgg taatgatcca ttaagttagt gtgactaagg
     4621 gcaaacgcca aattgcctga tgcgttacgc gcatcaggca agataaggcg ctccgggtca
     4681 acaatctaag ggcaattctc tgatgaggat tgcccttttc tttaccagac atctcccccc
     4741 acaagaattg gccctaccaa ttcttcgctt atctgacctc tggttcacaa ttccccaatt
     4801 aaaactcaca tcaatattgc taatacataa catttagtta accattcatt gtcattatcc
     4861 ctacacaaca caattggcag tgccactttt acacaacgtg tgacaaggag atgagcaaca
     4921 gactcattac acgatgtgcg tggactctag gagacctgca atgaatctct ggcaacaaaa
     4981 ctacgatccc gccgggaata tctggctttc cagtctgata gcatcgcttc ccatcctgtt
     5041 tttcttcttt gcgctgatta agctcaaact gaaaggatac gtcgccgcct catggacggt
     5101 agcaatcgcc cttgccgtgg ctttgctgtt ctataaaatg ccggtcgcta acgcgctggc
     5161 ctcggtggtt tatggcttct tctatggttt gtggcccatc gcgtggatca ttattgcagc
     5221 agtgttcgtc tataagatct cggtgaaaac cgggcagttt gacatcattc gctcgtctat
     5281 tctttcgata acccctgacc agcgtctgca aatgctgatc gtcggtttct gtttcggcgc
     5341 tttccttgaa ggagccgcag gctttggcgc accggtagca attaccgccg cattgctggt
     5401 cggcctgggt tttaaaccgc tgtacgccgc cggactgtgc ctgattgtta acaccgcgcc
     5461 agtggcattt ggcgcgatgg gcattccaat cctggttgcc ggacaagtaa caggcatcga
     5521 cagctttgag attggccaga tggtggggcg tcagctaccg tttatgacca ttatcgtgct
     5581 gttctggatc atggcgatta tggacggctg gcgcggtatc aaagagacgt ggcctgcggt
     5641 cgtggttgca ggcggctcgt ttgccatcgc tcagtacctt agctctaact tcattgggcc
     5701 ggagctgccg gacattatct cttcgctggt atcactgctc tgcctgacgc tgttcctcaa
     5761 acgctggcaa ccagttcgcg tattccgctt cggtgacttg ggggcgtcac aggttgatat
     5821 aacgctggcc cacaccggtt acaccgcggg tcaggtgtta cgtgcctgga caccgttcct
     5881 gttcctgacc gctaccgtaa cactgtggag catcccgccg tttaaagccc tgttcgcatc
     5941 gggtggtgcg ctgtatgagt gggtgatcaa cattccggtg ccgtacctca ataaactggt
     6001 tgcccgtatg ccaccagtgg tcagcgaggc tacagcctat gccgccgtgt ttaagtttga
     6061 ctggttctct gccaccggca ccgccattct gtttgctgca ctgctctcga ttgtctggct
     6121 gaagatgaaa ccgtctgacg ctatcagcac cttcggcagc acgctgaaag aactggctct
     6181 acccatctac tccatcggca tggtgctggc gttcgccttt atctcgaact attccggact
     6241 gtcatcaaca ctggcactgg cactggcgca caccggtcat gcattcacct tcttctcgcc
     6301 gttcctcggc tggctagggg tattcctgac cgggtcggat acctcatcta acgccctgtt
     6361 cgccgcgctg caagccaccg cagcacaaca aattggcgtc tctgatctgt tgctggttgc
     6421 cgccaatacc accggtggcg tcaccggtaa gatgatctcc ccgcaatcta tcgctatcgc
     6481 ctgtgcggcg gtaggcctgg tgggcaaaga gtctgatttg ttccgcttta ctgtcaaaca
     6541 cagcctgatc ttcacctgta tggtgggcgt gatcaccacg cttcaggctt atgtcttaac
     6601 gtggatgatt ccttaatgat tgttttaccc agacgcctgt cagacgaggt tgccgatcgt
     6661 gtgcgggcgc tgattgatga aaaaaacctg gaagcgggca tgaagttgcc cgctgagcgc
     6721 caactggcga tgcaactcgg cgtatcacgt aattcactac gcgaggcgct ggcaaaactg
     6781 gtgagcgaag gcgtgctgct cagtcgacgc ggcggcggga cgtttattcg ctggcgtcat
     6841 gacacatggt cggagcaaaa catcgtccag ccgctaaaaa cgctgatggc cgatgatccg
     6901 gattacagtt tcgatattct ggaagcccgc tacgccattg aagccagcac cgcatggcat
     6961 gcggcaatgc gcgccacacc tggcgacaaa gaaaagattc agctttgctt tgaagcaacg
     7021 ctaagtgaag acccggatat cgcctcacaa gcggacgttc gttttcatct ggcgattgcc
     7081 gaagcctcac ataacatcgt gctgctgcaa accatgcgcg gtttcttcga tgtcctgcaa
     7141 tcctcagtga agcatagccg tcagcggatg tatctggtgc caccggtttt ttcacaactg
     7201 accgaacaac atcaggctgt cattgacgcc atttttgctg gtgatgccga cggggcgcgt
     7261 aaagcaatga tggcgcacct tagttttgtt cacaccacca tgaaacgatt cgatgaagat
     7321 caggctcgcc acgcacggat tacccgcctg cccggtgagc ataatgagca ttcgagggag
     7381 aaaaacgcat gattatttcc gcagccagcg attatcgcgc cgcagcgcaa cgcattctgc
     7441 cgccgttcct gttccactat atggatggtg gtgcatattc tgaatacacg ctgcgccgca
     7501 acgtggaaga tttgtcagaa gtggcactgc gccagcgtat tctgaaaaac atgtccgact
     7561 taagcctgga aacgacgctg tttaatgaga aattgtcgat gccggtagca ctggcaccgg
     7621 tgggtttgtg tggcatgtat gcgcgtcgtg gcgaagttca ggcagccaaa gcggcggacg
     7681 cgcatggcat tccgtttact ctctcgacgg tttccgtttg cccgattgaa gaagtcgcgc
     7741 cagccatcaa gcgcccaatg tggttccagc tttatgtact gcgcgatcgc ggctttatgc
     7801 gtaacgcgct ggagcgagca aaagcagcgg gttgttcgac gctggttttc accgtggata
     7861 tgccgacacc gggcgcacgc taccgtgatg cgcattcagg tatgagcggc ccaaatgcgg
     7921 caatgcgccg ctacttgcag gcggtgacgc atccgcaatg ggcgtgggat gtgggcctga
     7981 acggtcgtcc acatgattta ggtaatatct cagcttatct cggcaaaccg accggactgg
     8041 aagattacat cggctggctg gggaataact tcgatccgtc catctcatgg aaagaccttg
     8101 agtggatccg cgatttctgg gatggcccga tggtgatcaa agggatcctc gatccggaag
     8161 atgcgcgcga tgcagtacgt tttggtgctg atggaattgt ggtttctaac cacggtggcc
     8221 gccagctgga cggtgtactc tcttccgctc gtgcgttgcc cgctattgca gatgcggtga
     8281 aaggtgatat cgccattctg gcggatagcg gaattcgtaa cgggcttgat gtcgtgcgta
     8341 tgattgcgct cggtgccgac accgtactgc tgggtcgtgc tttcctgtat gcgctggcaa
     8401 cagcgggcca ggcgggtgta gctaacctgc taaatctgat cgaaaaagag atgaaagtgg
     8461 cgatgacgct gactggcgcg aaatcgatca gtgaaattac gcaagattcg ctggtgcagg
     8521 ggctgggtaa agagttgcct acggcactgg ctccgatggc gaaagggaat gcggcatagt
     8581 cgtttgcgcc ccctcaccct aaccctctcc ctcagggaga ggggaccgtt cggcgctgta
     8641 tgtactccct cactctgaaa cgacaccgca ctcttttttt ctccctcgcc cctccgggga
     8701 gggccggggt gaggggaaaa ggccgcacgg ttcaccaatt ttctgctatc ctgcccccgc
     8761 actaaggggg cagtatgcta aacatcgtac tttacgaacc agaaattccg ccaaatactg
     8821 gcaacatcat ccgtctttgc gctaataccg gctttcgtct acatatcatc gaaccgatgg
     8881 gatttgcctg ggacgacaag cgcctgcgcc gcgcggggct ggactatcac gagtttaccg
     8941 ccgttacgcg tcatcatgac tatcgcgcgt tcctcgaagc agaaaatccc cagcgcctgt
     9001 ttgccctcac cacgaaaggt acgcctgctc acagcgccgt aagctatcag gatggcgatt
     9061 atctgatgtt cggcccggaa acacgcggcc tgccagcgag cattcttgat gccctgcccg
     9121 ctgaacaaaa aattcgcatt ccgatggtgc cggacagccg cagcatgaat ctgtccaatg
     9181 cggtgtcggt agtggtgtat gaagcctggc ggcagctggg atatccggga gcgttgttga
     9241 gagattagtt actgtatgcc ggatagatga ttacatcgca tccggcacga tcacaggaca
     9301 ttagatccca tccccatact caaatgtatg gttaataccg ttgaaatgct ggtccatatc
     9361 cattgatggc ttatcgctgt ctggtttacc gacaatacga gccggaacgc cagcggcggt
     9421 tgtatgcggc ggcaccggtt gcagcaccac ggaacctgcg ccaatcttcg cgccgcgacc
     9481 aacttcaata ttgccgagga ttttcgcgcc cgcgccaatc atcacacctt cacgaatttt
     9541 cgggtgacgg tcaccaccag atttacccgt accgccaagc gtcacagatt gcagaatcga
     9601 tacgtcgttt tcaattaccg ccgtttcacc cacaacgatg cccgtggcgt ggtcgagcat
     9661 aatgccacgg ccaatttttg ctgccgggtg aatatcgacc tggaacgtca cagaaacctg
     9721 gttttgcaga aagattgcca atgcgcgacg cccctgattc cacaaccagt gaccgatgcg
     9781 ataggcctgc aaggcatgaa aacccttcag gtataacaac ggggttgagt atttatcgac
     9841 tgccgggtcg cgggtacgca ccgcctgaat atcacaggcc gcagaggcga tcatttccgg
     9901 gtcagcggcg taggcttctt caaccacttc acggatagca atagcaggca taattggcga
     9961 tgacagcttg ttcgccagca tgtagctcag tgcactgcca aggttttcgt gcttgagtag
    10021 cgtcgcgtgg taaaaactgg ccagcattgg ctcacagtcc gccagcgttc tggcttcggc
    10081 tttaatattg ttccagacaa tttccagttc ttcacacgac attgcttact ccacacgatg
    10141 agataatgac cggcccgttc tgcgcgggcc gggtcatagc ggtaacaaag gttccctggg
    10201 gttagtggct gctgcgctcg tccttgcgtg cacgacctaa taatgtcaat gctgcctcgc
    10261 gcgcgttttt tccgcaatat aatacttgat aaatttcctc ggttattggc atttcaacgc
    10321 cgaagcgatg cgccagttcg cggacttctt tcgtattgcg gtagccttcc accacctgac
    10381 caatcttctc ctgcgcgctt tgtacatcca tgccctgacc gagcatcatg ccaaaacggc
    10441 ggttacgcga ctggttgtcg gtacaggtaa gcaccagatc gcccagcccc gccatgccca
    10501 taaaggtggc aggatcggca cccagcgccg caccaagacg cgacatttca gccagcccac
    10561 gggtgatcag cgccgtacgc gcattcgcac caaaaccgat accgtcagac atccccgcgc
    10621 caatggcaat gacgtttttc accgcgccgc caagctgcac gccaatgaaa tccgggttgc
    10681 tgtaaacgcg gaaacttttg ccgcagtgca gcagctgctg gagatcatcg gcaaaggtct
    10741 gatcggtcga ggccagcgaa atagctgtcg gtaaacctgc cgccagttct ttcgcaaacg
    10801 ttgggccaga gataaccgcc agcggaattt gatcgcctaa ggcctcacgc gccacgtcct
    10861 gtaacagacg tccggtttcc gcttccagcc ctttggtcgc ccacaccaga cgcgcatcag
    10921 gacgcatcag cggtttaatc tggcgcagca cttcaccaaa gacatggctg ggtacgacga
    10981 cgagaatatt acgactggct gccagcgcag tggcgagatc gctttcaaga tggagcgtat
    11041 cgggaaaagg cacatcgggg agaaacgcgg cgttacagcg gtcgcgttca agcgttgcga
    11101 tatgttcagg gtcatggccc cagaggacaa cctcgtggcc atttcttgcc agggtaatgg
    11161 caagagcggt gccgtacgag ccggcaccga tcacagtcat tgaagcatta cgttggttca
    11221 tcaggcatcc tgatgttctt cagtaccttc gccagcctgc tgctgcaaat agttcatgaa
    11281 cagcgcatcg aagttaaccg gcgcaaggtt cagttgcggg aatgtaccgc gggataccat
    11341 gctggtgatg cactcacgag catacgggaa cagaatgttc gggcagtatg cgcccaggca
    11401 atgtgccatc tgggtgcctt cgatacccgc gatggagaaa ataccgccct gctgaacttc
    11461 acacaggaac gcggtttctt cgcccaagga ggccgttacg gtaacacgca gtaccacttc
    11521 gtatacgtca tctgccagtt gggaagatgc cgtatccaga tcaagtttaa cttctggttg
    11581 ccaatctttc tggaaaacgt gcggcgcgtt cggcgcttcg aaagagatat ccttggtata
    11641 aatacgttgg atctggaaag tcatttcagt gttgttttgt tctgacatgt gtagaaaacc
    11701 cttaagtgtt gtccttaaat actgcgcaat gtaatgccaa cgttcacacg ttatttcagc
    11761 aggggatcca gtccaccacg tgcatccaat gcatacaagt catcacagcc gccaatgtgc
    11821 tgtgcgtcaa taaaaatctg gggaaccgtg gtgcgaccgc tgcgtttgat catctcttca
    11881 cgcttggcgg cattgccatc gatcggcaac tcctggaaac tcacgccctt gctgctcagc
    11941 agtgcttttg cacgatggca atacgggcag gtttctttgg tatagatttc aacattggcc
    12001 ataatttagc tcctgttttt ttaccctgaa atttcatgtt gcagggcggc tgcaaaatgg
    12061 agtgtcccca gaagcttata acaataagtc gctgggggcc taattgcagc taacggcctg
    12121 catcatgaaa gacgacaggt aaattatttg ccgcgcacca gaggcaagtt ttcgccagcc
    12181 cagccagcaa cgccctcttt cagcacgaat acttgcgcaa aaccagcttt cgtcagcgcg
    12241 tttgcaggct cctggcactg catgccagaa ccgtctacca cgataaccga tttgtctttg
    12301 tgcttctcaa gctcaccaac attgttggct ttgatttcgc tcggcaacag attgatagaa
    12361 cctgcgatat ggcctttacg gaagtcatca cgctgacgta aatccacaac cacagcgtct
    12421 tctttattga tcagacgcgt agcttcacca cgagtaatca ccttcacttt cgaggtcagg
    12481 ctcttaaacg tagtcacaag aaccgccacc agtaacgcga tccaggcgat actcagtatg
    12541 ggatgacggc caacaaattg cataatttct tgcatggggg gtaacaactc ccaacgtagt
    12601 gattaaaaaa accaggaaag gagtatacct gcgcggtgcg gcaaatacag ccagcgcgtt
    12661 aactggaatg caattttgcg gggcacgacg aaaaaaaagc gcaaatctgg catcggcttg
    12721 ccgcgagcgg tcgtatattt tgatcttcag aggctatttt atcgattcag ctgtagtaaa
    12781 attacgcaaa ttttgactct tgagtatgag gttgtcgcaa tgtcggtttc taaaaaacct
    12841 atggtactgg tgattctgga tggctatggc tatcgtgaag aacagcagga taacgccatt
    12901 tttagtgcta aaaccccggt aatggatgca ctgtgggcca atcgtccgca taccctgatc
    12961 gacgcttccg gtctggaagt cggtctgcct gaccgtcaga tgggtaactc cgaagtaggc
    13021 cacgttaacc tgggtgccgg ccgcatcgtg tatcaggacc tgactcgtct ggacgttgaa
    13081 atcaaagatc gcgctttctt tgctaatcca gtgctggctg gcgcggtaca taaagcgaaa
    13141 aacgcaggta aagcagtaca cattatgggt ctgctctccg caggcggcgt acacagccac
    13201 gaagatcaca tcatggcgat ggtagaactg gcagctgaac gcggcgcaga aaaaatctac
    13261 ctgcacgcat tccttgacgg tcgcgacacc ccgccgtgca gtgctgaatc ctcgctgaaa
    13321 aaatttgaag agaaatttgc tgcgctgggt aaaggccgcg tagcgtccat cattggtcgt
    13381 tactacgcga tggaccgcga taaccgttgg gatcgcgttg aaaaagccta tgacctgctg
    13441 actctggcgc agggtgagtt ccaggccgat actgccgttg ctggtttgca ggcagcttat
    13501 gctcgcgacg aaaacgacga gttcgtgaaa gcgaccgtta ttcgtgcaga aggtcagcca
    13561 gatgcggcaa tggaagacgg cgatgcgctg attttcatga acttccgtgc tgaccgcgcg
    13621 cgtgaaatca ctcgtgcatt cgtgaacgct gatttcgatg gcttcgcgcg taagaaagtg
    13681 gttaacgtcg atttcgtgat gctgaccgaa tacgccgctg acatcaaaac tgcggttgct
    13741 tacccacccg cttccctggt taacaccttc ggcgagtgga tggcgaaaaa cgacaaaact
    13801 cagttgcgta tttccgaaac cgaaaaatat gcccacgtta ctttcttctt caacggtggc
    13861 gtagaagagt cgttcaaagg cgaagatcgc attctgatca actcaccgaa agtggctacc
    13921 tacgatctgc aaccggaaat gagctccgca gagctgaccg aaaaactggt tgcggccatc
    13981 aagagcggca aatacgacac cattatctgt aactatccga acggcgacat ggtaggtcac
    14041 accggggtga tggaagcggc ggttaaagcg gttgaagcac tggatcactg cgtggaagaa
    14101 gtcgcgaaag cggttgagtc cgtgggtgga caactgctga tcaccgctga ccacggtaac
    14161 gctgaacaga tgcgcgatcc ggcaacgggt caggcacaca cggcacacac caacctgcca
    14221 gttccgctga tttacgttgg tgataagaac gtgaaagcgg ttgcaggcgg caaactttct
    14281 gacatcgcgc cgaccatgtt gtcgctgatg ggtatggaaa tcccgcaaga gatgactggt
    14341 aagccgctgt tcatcgtgga ataatccctc cccatgaggg gaaaggcgat taataccatg
    14401 acacgggccg tgaaaccgcg caggtttgca atcaggccca tcatctacgc cagcgttctt
    14461 agcgctggcg tattgttgtg cgccttttcc gcccacgcgg atgagcgtga ccaactcaaa
    14521 tctattcagg ccgatatcgc cgcaaaagag cacgcggtac gccaaaagca acaacaacgc
    14581 gcaagcctgc tcgcacaatt gaaaaagcag gaagaagcga tctctgaagc cacccgtaag
    14641 ctgcgcgaaa cgcaaaacac gctcaatcaa ctcaataaac agattgatga gatgaacgcg
    14701 tcgattgcca aactggagca gcaaaaagcc gcccaggagc gcagcctcgc cgcacaactg
    14761 gatgccgcat tccgtcaggg cgagcatacc ggtattcagc taattctcag cggtgaagaa
    14821 agccagcgtg gacagcgttt acaggcttat ttcggctatc tcaaccaggc gcgacaagaa
    14881 accattgccc agttgaagca aacgcgtgaa gaagtcacca tgcagcgtgc tgaactggaa
    14941 gagaaacaaa gcgagcaaca aacgctgtta tatgaacagc gcgcccaaca ggcgaaactg
    15001 actcaggttc tgaacgagcg taaaaagacg ctggcggggc tggaatcttc catccagcaa
    15061 ggtcagcagc agctaagtga actgcgtgcc aacgaatccc gtctgcgtaa cagcattgcc
    15121 cgtgcggaag ccgcggcgaa agcgcgtgct gaacgagaag cgcgtgaggc ccaggcggtt
    15181 cgcgagcgcc agaaagaagc gacgcgcaaa ggcaccacct acaaaccgac cgaaagcgaa
    15241 aaatcgctga tgtcccgaac tggtggcctg ggtgcgccgc gtggtcaggc attctggccg
    15301 gttcgcgggc cgacgctgca tcgctatggc gagcagctac agggtgaatt acgctggaaa
    15361 gggatggtta tcggtgcctc tgaaggtact gaagttaaag caattgccga cggtcgagtg
    15421 attctggctg actggctgca aggttacggt ctggtggtgg tggttgagca cggtaaaggc
    15481 gacatgagtc tttacggcta taatcagagc gcactggtga gcgttggttc gcaggttcgc
    15541 gcgggccagc caattgcact ggtgggcagc agtggcggtc agggtcggcc ttcactctat
    15601 ttcgaaattc gccgccaggg tcaggcggtc aatccacagc cgtggttggg aagataagtt
    15661 ttgtttccat ttcgtcgtaa cgttcttgca tttgccgctc tgttggcgct ctcctccccc
    15721 gtacttgctg gcaaacttgc catcgtcatt gatgattttg ggtatcgccc gcacaacgaa
    15781 aaccaagtgc tggcgatgcc ttccgctatc tccgtcgctg tattacccga ttcaccgcac
    15841 gccagagaga tggcgaccaa agcgcataac agcggtcacg aagtgttgat tcatctcccg
    15901 atggcaccgt tgagtaaaca gccgctggag aaaaatacgc tacgcccgga gatgagcagc
    15961 gacgaaattg agcgcattat tcgtagtgcg gtcaataacg tgccctatgc cgtggggatc
    16021 aacaaccaca tgggcagcaa gatgacctct aacctgtttg gtatgcagaa agtgatgcag
    16081 gcgctggagc gttacaatct ttacttcctc gacagcgtaa ccatcggtaa tacccaggcg
    16141 atgcgcgccg cgcaaggtac tggcgtgaag gtgatcaaac ggaaggtgtt cctcgacgat
    16201 tcgcagaacg aagcggacat ccgtgtgcaa tttaatcgcg caattgatct ggcgcgtcgc
    16261 aacggttcaa ccattgccat tgggcatcct cacccttcaa cggtacgcgt gctacaacag
    16321 atggtttata acctgccgcc agacattacg ctggtgaaag ccagcagctt gctgaatgaa
    16381 ccgcaggttg atacttctac accgccgaaa aacgctgtgc ctgatgcacc gcgtaatccg
    16441 ttccgtggcg tgaagctgtg caaaccgaag aaaccgatag aacctgttta tgctaatcgc
    16501 ttctttgaag tattaagcga aagcatcagc cagagcacgc tgatcgttta cttccagcat
    16561 cagtggcaag gctggggcaa acagcacgaa gcggcgaagt ttaacgctag cgcaaattaa
    16621 aggcgctacg cgtaatgcga tggtgcgacc gcgttttgtc gcgccattgc cataagcgga
    16681 aagaccacag taacgcctgg taaccgacct tcacgctgcg cacattggta atcaggcgct
    16741 tatacatacc ggaagtaaat atctcggcaa tcatccgttg gcgggtaaga atatccggct
    16801 ctttgcgcac cgcatggcaa acacgcaatg cttcgtaagt tatttgctga tgaaattccg
    16861 gataaatcgt aattttgtcg gcataatttc gatttaattt ctccagcagg cgggtaatct
    16921 taatatagtg acgttgataa ttaaggtttt tattcccttg tctatgcaac cgactcactg
    16981 acgtattatg cagataatat ttatataatg attgctcggt atatcgcgca cgcagcgcgt
    17041 taaacatgaa ttctgttgtc cagacaatat cctgatgatg taatccggca ataaatttaa
    17101 tgttattttt aacaataaca tcacggcgat aaacccccat ccagacaacg tgagtccaac
    17161 ggcgcgaaga aagccccatc cgcagccagt ccgggccggt taatacaccg gttgagcgaa
    17221 ggcgatcgct ggggatggat tgccaggttt ctcccgtttc acgaaaagac cagtcagcgt
    17281 tgcactgcgc cacgtcgagg tcgtcctcta acgccatggt catcagcgtt tcgtacatgg
    17341 tgggatagac ctcatcgtca gcatcgacaa aagcgacata tttgcccgtc gccacctcga
    17401 tcccacgatt acgcgctacc gatgcgccag cattcgcctg atgcaacaaa cgaacgtgcg
    17461 gatagttttc tgcgtaatgc tttgctattt caacagaatt atccgttgaa ccatcgttaa
    17521 taataatgat ttccagagca gtccaggttt gcgtaattaa agattccata caagtgcgga
    17581 aatcatcgcc cgcattatat aacggaataa taacactaag tttattggtg ctgttcatca
    17641 taaattaccg acgacctctg aagttatcct gacgttttac aggctttgta ttaagaaact
    17701 attaagcctg tgagatatta tttttacagg tgagagagtc acttcagggt agagagataa
    17761 tgagaacagc tatataaaag atcagcgcaa ttacgaactc tttattacac tgacaggatt
    17821 ttacataatt aacagattat cctcagataa aagggctgga ataccagccc ttgttcgtgt
    17881 taatcccagc tcagaataac tttcccggac tggcccgaac gcatagcgtc aaagcccttc
    17941 tggaaatcat cgatagagaa acgatgggta atgatcggcg agagatcgag gccagactga
    18001 atcagcgccg ccatcttgta ccaagtttca aacatctcac gaccgtaaat gcctttaatg
    18061 aacaagcctt taaagatcac tttggtccag tcgatagaca tatcagacgg tggaataccc
    18121 agcatcgcaa tacgaccgcc gtggttcatg gtatcgagca tggtacgaaa cgctggcggc
    18181 gcgccggaca tttccagacc gacatcaaag ccttcggtca tgcccagttc agccatcaca
    18241 tcattaaggt tttctttcgc aacgttaacc gcacgggtga tacccatttt gcgcgccagc
    18301 tcaaggcggt attcgttaac atcggtgatc accacattgc gtgcaccaac gtgtttcgcc
    18361 accgccgcag ccataatacc tatcggacct gcaccagaaa ccagcacatc ttcgcccacc
    18421 agatcgaacg acagcgcggt atgcacggcg ttaccgaagg gatcaaaaat ggaagccagg
    18481 tcgtcggaga tattgtcggg gattttgaaa gcgttgaacg ccggaatcac cagatattcg
    18541 gcgaagcagc ccggacggtt aacaccgaca ccaatggtgt tgcggcacag atgggtacga
    18601 ccgccacgac agttgcgaca gtgaccgcag gtgatatggc cttcgccaga aacgcgatcg
    18661 ccgattttga agcctttcac ttcctgacca atacctacca cttcaccgac atattcatgg
    18721 cccacgacca tcggcaccgg gatggttttt tgcgaccact catcccagtt atagatgtga
    18781 acgtcagtcc cgcagatggc tgttttacgg attttaatca gcagatcgtt atgcccgagt
    18841 tccggtacag ggacgtcggt catccagatg ccctcttccg ctttcagttt ggataacgct
    18901 ttcatctcac atcctcaggc gataacgccc agttgtttac caatacgcgt gaatgcttct
    18961 actgcacgcg taatttgctc aggggtatgc gccgcagaca tctgggtacg aatacgcgcc
    19021 tgacctttcg gaacgaccgg atagaagaaa ccggtgacgt aaatgccctc tttttgcagc
    19081 tctcgggcaa atttctgcgc cactaccgca tcaccaagca tgaccggaat aatggcgtga
    19141 tcggctcccg ccagggtaaa gcccgccgcc gacatttgct cacggaactg acgcgcgttc
    19201 gcccacagac ggtcacgcaa ctcgctaccc gcttcaacca tctccagcac tttgatggaa
    19261 gcggcgacaa tcgctggtgc cagcgagttg gagaacaggt acggacgaga acgctggcgc
    19321 agccactcaa ccacttcttt gcgcgccgcg gtataaccac cagaagcccc gcccagcgct
    19381 ttaccgagcg tgccggtgat gatgtcgaca cgccccatca catcgcagta ttcatgggaa
    19441 ccacgaccat tttcaccgac aaaaccgacc gcgtgggagt cgtctaccat caccagggca
    19501 tcgtatttat ctgccagatc gcaaacgccc ttcagattgg caatcacgcc gtccatcgag
    19561 aacacgccat cggtggcaat cagcacatga cgcgcaccgg cttcacgcgc ttctttcaga
    19621 cgatcttcaa gctcctgcat atcgttgttg gcatagcgat agcgtttagc tttacacaga
    19681 cgcacaccat caataataga cgcgtggttc agtgcgtcgg agataatggc gtcttccgcg
    19741 cccagcagcg tttcaaacag gccaccgtta gcatcaaagc aggaagagta gagaatcgca
    19801 tcttccatcc ccaggaatgc cgccagtttt tgttcaagct ctttatggct gtcctgagtg
    19861 ccgcaaataa aacgcaccga agccatgccg aaaccgtgag aatccattcc cgcctttgct
    19921 gccgcaatca gatccggatg attcgccagc ccgagatagt tgttggcaca aaagttaatg
    19981 acgtggcttc catcagccac ggtgatatct gcttgctgcg cagacgtaat aatgcgctct
    20041 tctttaaaca acccttccgc ccgtgcggtt tccagatcgt tggttaactg ctgataaaat
    20101 tctccacgca ttgcgattct ccagacaggg caaattccag cacatattac ccaaacttat
    20161 aggtcgcgac gagatgatgc gttaacactt ctgcaaaatt caggataaat aacgtgtatc
    20221 ttgatgtctc tcaacaccta cagatagtag aaaaactgta cagttttggc tgagcataat
    20281 gatgagtgat tttactgcat tgatataaaa agaaaaaatg atgccaaagc atcatttttc
    20341 ctatctcagt tggcatcaaa acgctaccca ctaatggagg gaaacctatt ttcttgaaag
    20401 gaaaattctc agcgcctgca tttttgcccc taaagtgtta ctcctgaagc agcgaaacaa
    20461 atcgaaccat tttcctctcc ccagatagtt taggttgaag agatcggaac gtttgtaata
    20521 gcacatgaca aaaatgccct ggtcatcgtc cacaatgtta ttgtttagtg ttattttttg
    20581 actttccaga actaatttat agaattcttt ccatttatgc tgtgaaccaa caattgcgcc
    20641 cccaataata tatacatgat tgccaatcat gaaatcaaaa gcctgttgtt gagatgtaac
    20701 agtaagccct ttttttatag tgaaaagatg catcttattt tcatcaaatg ggaaatccca
    20761 aattttcaat cctcgagtta catttggctt acggcaatag ccaaaatcta tccacgcaac
    20821 aagaggcgtt ttcactaaac ccatgttgat agctttattt acaaaatagg ccttaaggtt
    20881 acataccaaa acatactctg gtgaccagta ctctgggttt tttaattgtc gaggctcaag
    20941 tctatttgta aacgactcat ctttttgaat tttttcgata cgatttctga tatatctaaa
    21001 tttctttttt agatcgataa cgataaccgt tgttggtttt ccgttgcgaa tcgcttcaat
    21061 ccttggtttc aaatcgggag aagtgaaaat aatcatctca ttttcaagtg cggccagacg
    21121 ctcaaagtaa ctaaaataaa catcaactga acgagctagt ttttcacgga accctttatt
    21181 agcagtccaa tcccctctac caatgtcaaa atatgcagtg ataattgtgg tggatgattt
    21241 cattactaat aatgaccatt aatataaaaa aagaattata tcataaatat taacagaatg
    21301 tatactgtat attatccatt caaatgattc cgttatttat catacatcgg aatattgata
    21361 ctaaagcact attcacatgc aaaaccaaca tccgccatga aggactagct aaaacccaaa
    21421 ctagtttgtt gcaattagca tccttgcacc tctatgtaaa gggctgaagg gattcggatg
    21481 tgatggtatg attacagaca ttcgtgtctg agattgtctc tgactccata attcgaaggt
    21541 tacagttatg atcatcgtta ccggcggcgc gggctttatc ggcagcaaca tcgttaaggc
    21601 cctgaatgat aaaggcatca ccgatattct ggtggtggac aacctgaaag acggcaccaa
    21661 gtttgtgaac ctggtggatc tggatatcgc ggactatatg gataaggaag acttcctgat
    21721 ccagattatg gctggcgaag agttcggtga tgtcgaagcg attttccacg aaggcgcatg
    21781 ctcttccacc accgagtggg atggcaagta tatgatggat aacaactatc aatactccaa
    21841 agagctgctg cactactgtc tggagcgcga aatcccgttc ctgtatgcct cttccgcagc
    21901 cacctacggc ggacgcacct ccgactttat tgaatcccgc gagtacgaaa aaccgttgaa
    21961 tgtctacggt tactcaaaat tcctgtttga tgaatatgtt cgtcaaatcc tgccagaagc
    22021 gaactcgcag attgttggct tccgctattt caacgtttat ggaccgcgtg aaggccataa
    22081 aggcagcatg gcgagcgtcg ctttccatct caacacccag cttaataacg gtgaatcacc
    22141 gaagctgttc gaaggtagcg agaacttcaa acgtgacttc gtctatgtgg gcgacgtggc
    22201 agatgtaaac ctgtggttcc tggaaaatgg cgtttccggt atcttcaacc tcggcaccgg
    22261 tcgtgcagaa tccttccagg cagtagcaga tgctacgctg gcttatcaca agaaaggcca
    22321 gatcgaatac attccgttcc cggataagct gaaaggccgc taccaggcgt tcactcaggc
    22381 agatctgaca aatctgcgcg cggcgggtta cgacaaaccg ttcaaaaccg ttgctgaagg
    22441 tgtaacggaa tacatggcct ggctgaatcg cgacgcataa gagctctgca tgaaaatact
    22501 ggtgatcggc ccgtcttggg ttggcgacat gatgatgtcg caaagtctct atcgcacgct
    22561 ccaggcgcgc tatccccagg cgataatcga tgtgatggca ccggcatggt gccgtccatt
    22621 attatcgcgg atgccggaag ttaacgaagc tatccctatg cctctcggtc acggagcgct
    22681 ggaaatcggc gaacgccgca aactgggtca tagcctgcgt gaaaagcgct acgaccgcgc
    22741 ctacgtctta ccaaactcct tcaaatctgc attagtgcct ttcttcgcgg gtattcctca
    22801 tcgcaccggc tggcgcggcg agatgcgcta cggtttactc aacgatgtac gcgtgctcga
    22861 taaagaagcc tggccgctaa tggtggaacg ctatgtcgcg ctggcctatg acaaaggcat
    22921 tatgcgtacc gcacaagatc tgccgcagcc attgttatgg ccgcagttgc aggtgagcga
    22981 aggtgaaaaa tcatatacct gtaatcaatt ttcgctttca tcagaacgtc cgatgattgg
    23041 cttttgcccg ggtgcggagt ttggtccggc aaaacgctgg ccacactacc actatgcgga
    23101 actggcaaag cagctgattg atgaaggtta tcaggtggtt ctgtttggct ctgcgaaaga
    23161 tcatgaagcg ggcaatgaga ttcttgccgc tttgaatacc gagcagcagg catggtgtcg
    23221 gaacctggcg ggggaaacac agcttgatca agcggttatc ctgattgcag cctgtaaagc
    23281 cattgtcact aacgattctg gcctgatgca cgttgcggcg gcgctcaatc gtccgctggt
    23341 tgccctgtat ggtccaagta gcccggactt cacaccgccg ctatcccata aagcgcgcgt
    23401 gatccgtctg attaccggct atcacaaagt gcgtaaaggt gacgctgcgg agggttatca
    23461 ccagagcttg atcgacatta ctccccagcg cgtactggaa gaactcaacg cgctattgtt
    23521 acaagaggaa gcctgacgga tgcgggtttt gatcgttaaa acatcgtcga tgggcgatgt
    23581 tctccatacg ttgcccgcac tcactgatgc ccagcaggca atcccaggga ttaagtttga
    23641 ctgggtggtg gaagaagggt tcgcacagat tccttcctgg cacgctgccg ttgagcgagt
    23701 tattcctgtg gcaatacgtc gctggcgtaa agcctggttc tcggccccca taaaagctga
    23761 acgcaaagcg tttcgtgaag cgctacaagc agagaactat gacgcagtta tcgacgctca
    23821 ggggctggta aaaagcgcgg cactggtgac acgtctggcg catggcgtaa agcatggatt
    23881 ggactggcaa accgctcgcg aacctttagc cagcctgttt tacaattgta agcatcatat
    23941 tgcaaaacag cagcacgccg tagaacgcac ccgcgaactg tttgccaaaa gtttggacta
    24001 tagcaaaccg caaacccagg gcgattatgc tatcgcacag cattttctga cgaacctgcc
    24061 tacagatgct ggcgaatatg ccgtatttct tcatgcgacg acccgtgatg ataaacactg
    24121 gccggaagaa cactggcgag aattgattgg tttactggct gattcaggaa tacggattaa
    24181 acttccgtgg ggcgcgccgc atgaggaaga acgggcgaaa cgactggcgg aaggatttgc
    24241 ttatgttgaa gtattgccga agatgagtct ggaaggcgtt gcccgcgtgc tggccggggc
    24301 taaatttgta gtgtcggtgg atacggggtt aagccattta acggcggcac tggatagacc
    24361 caatatcacg gtttatggac caaccgatcc gggattaatt ggtgggtatg ggaagaatca
    24421 agtagagtgt cgttctacaa gtatgtctct tgcagatttg ccagctcaaa ccgtttttca
    24481 aaatttaaat cttgaaataa taaccaataa gttgacatcg gagataagat gacctcaaca
    24541 ttatttttct ctctcgagaa aaaaaactgg atagcgtact ggaacagagc tctcgtattc
    24601 ttattcatta ccacctattt tttgggtggg attacaaggt ataaacatct tattgttatt
    24661 cttatgacaa taacgacaat cgtctatctc tgcaaacggc caaaacacta tctctcacta
    24721 tttaaaacat ttctttttgg tagtgttgcc atattaacta ttgctgcatt gctgtcactt
    24781 cttcaatccc ctgatgcagg tgctagcatg aaggaaattt tcaaagctat tattgagaat
    24841 actttactat gcacaatagc aataccggtc atattgagag acgagaaaag agaagatgtc
    24901 gaaaaaatcg ttttcttctc atttattagt gcgttgggct tacgctgttt ttctgaattg
    24961 attacctatt ataaggacta tcaacaaggg ataatgccat tcgcagatta tagacaccgt
    25021 agcatttctg actcgatggt ctttttattc cctgcattgt taaatctctg gcttatcaaa
    25081 tcagcaaaat accgcatttc ttttgtggtt ctaagcgtta tttttatttt tctgatatta
    25141 ggaactttat ccagaggggc ctggctttcc gtgttagtca ttggattaat atggattctg
    25201 atgtttaaac aatggaagtt actattagta ggagtaatgg tttccatcat tgcattgtcg
    25261 gttattttca cacataagga gatgaccgca aagctaacgt ataaacttca acaaactaat
    25321 agttcttatc gctatgcaaa tggtactcaa ggcagcgcac tcgatctaat attagaaaat
    25381 cctgttattg gttatggtta cggtaacgtt gcatataaag atgtctataa taaacgtgtc
    25441 attgattatc cagaatggac ctttagacaa tcaatagggc cacataattt tgcgctattc
    25501 atctggtttg gcactggttt attagggctg gtaagtctta tgatgctata ctgtgcaata
    25561 ttgaaagagt gtataaaaaa tggcgtcaag aataaatatc gctcaccata taatgcatat
    25621 tatataatct tactatcttt tataggttat tttgttatcc gtggaaacgt agaacaaatt
    25681 gaaccaaatt tattaggcgt ttacgccggc ttattattag cgatgaaaaa caagtaaaaa
    25741 ataaaaaagg ctgcataatg cggccttttt atttattcaa accaattatg aataacctct
    25801 tcgaatcttt gagttacgcc atcccaactg tagtgatcaa acacaaagtc ctgaccttgc
    25861 tttgcaactg ctgttaattc aggattcgct aacgttttta aaatatcgct gcttatggag
    25921 tctgcagtca taggttcttt taaatggaat cccgtagtat tttctttgac aaattccgtc
    25981 atccccccac gcgtactcac taaaacaggc ttaccagcac ccatagcttc aattgccacc
    26041 atgcaaaatg gttcctggaa ctgggaagga atgacgacta aatctgccag tgggtaataa
    26101 caatgcatct tttcaggcgg tatgcttcct aacataatgc aacgatcctt caaacgtttt
    26161 gcaatttccc tgacattgcg ttgatatgca cctttgtcac ttttactcat ttctgtataa
    26221 tcaccaatca caactaattt taaatttttg tgagcagcgg ccaatttttc gaatgattgc
    26281 atcaacagca atatgccttt gtcagggacg atacgcccag cataaaaaat aattttctct
    26341 tcaggggaga tattaagttc gcttctagtg atcggctgaa aattagattg ataggtttct
    26401 aaatcaattc catttggtac aatctcaata tctgcatttg ctaaataaga ttggtaatac
    26461 tttttcaagt ataaactagg gactatcatt ttaacatttt gctcaagcaa ttttggttca
    26521 aatgcattat gcatatgtat aaccactctt gcctgaggag ctcgcttacg aatttgagtg
    26581 tatagcttca tgctattatg aactacaata acactttctt tcgttatagg aaaatcatgt
    26641 gcaatattca atattctctg tgaatatgga agaggatcaa gtcttgtcca tttttgaaat
    26701 aaccgcttat atatgcgact gaatccaacc ctatgaatac tacaattatc attaacaaag
    26761 gtgtaatttg agtatcctgg atttttgata caaacaatac gatttggaaa attggtacgc
    26821 tgagcaacct gatacatcca cgtttcaaca gcagccgctc cgcgaggagg aattgaaaaa
    26881 ataggagtaa ccgtaaatat tattttatca accatctcgt atattaacct ttcataacat
    26941 tatatttaat tgctgtaaaa acaccatcga gaaatttttt ctggtaaagc aagtgtttat
    27001 atttttcttt gtgctcatgt tttttaacag cttttttata aggtatattt ctccatggtg
    27061 atatattata aatattacga aaataatctg cagaggcgta gttcgcccaa tcatgccatg
    27121 gcttagttat accagtataa tgtatgaaga cagtgtcatc attaataaac cgggtgtaat
    27181 attcactatt tttttcttca aattctgact ttatagtata aatacaatta tattttctcg
    27241 gtaatatttt agcatgatgt agaaacatta agtttaaaat atcttgatcc gggtacttta
    27301 atttttttat aattgactca tctgataata gatcaaagaa tttttccgtt agtctttgtt
    27361 ttaaccattc ccgtaaatta atatacatta caccagagtt aaaatagcta ccgttaaaat
    27421 cctcattaca caaacgctca gcgcttttac tttgcatagc atctacatca attaccactg
    27481 ccccatattc atctttaaac tctaatgcta ttaactcgtt caatgaaccc ttacagacga
    27541 tatcagcatc aagatataat aatgaatcca atcgcgcaga aaaataatca aatgaaagca
    27601 aacgataata aatagatact ggccagatat ttgattgagg tagcgccttc aatggctcag
    27661 aatcaatatg atataattta attactgttt gatacttcgt tgctaattgt gataataatt
    27721 caatatactc tttgtctaag taatcagcga ttatgtgaaa ggtaaatttg ttttgtttat
    27781 tcttttcaag aattgaagca attgaaatcg cagccccaac ctggtagttc tcatcaatac
    27841 cccatgagac attaaacgtt tcccgttcat caagtttaac ggggcgcttg tccagttcaa
    27901 ttatatcttt aaattgagta agatgtttaa aatccaattt aattaccctt catcataatc
    27961 attatttgct cttcaatcct agtttgactt ttacatccct gattactttt ttaattttct
    28021 ttttgaatat aagatatgta tagccaaagt ctttcaattc attttttata ttatagtgcc
    28081 gttcaagatc aatgcgatct tttgctttta aaacagcggt tgttttttta cctgaaaggt
    28141 caattagtct taaacctttt tcggatacta taaaatttcc tttatgagga tcgccagaca
    28201 ccattccatg ctgatgcaac tcttttatcg attctgataa ttgatccttc agatcttcag
    28261 aaatttccaa atactcattt aacccgacac cttcaatata ctcaatgagc ataatataat
    28321 aatgagcgaa atttaatgtt ttacgttcag ctagcaaaaa atagtcatta attgattgga
    28381 tgccttcacc acgtacccga tcggtttgat aaattaaatt ttcataataa tcttttttta
    28441 tgcaagattt taagaaacgt tcggtcattt tatgctttgg cgcataaact ttaagtacaa
    28501 gaggtccacg agcagtgtca ataagaatga ctttagtatc atcaatagaa cgaaaaactt
    28561 ttaatgtttt aaccctgcaa gttaaaaagt catttagaac ttgctcataa aagggatcat
    28621 tatcttttat gaaaactttc aacccattta tcgttttatt atatatcata atgaattatt
    28681 ttaaccttaa atctttagaa gcatttttct ttataatact ttaaataatt aaaaatgcct
    28741 gccatataat gcttttgttt aaatttatgt tttgcacaat accgatactg attgctattt
    28801 acaggtttaa gtaaatcttc tttactccac ggagaagctg ctttggcgat caaaaaacta
    28861 cgtgaaaccg gataatcagc ccactcatgc cagggttttg taggtcccac atagtgtata
    28921 aacacggtgt catcattgac tggattatca actttgtctt ttaattcata gttaatacta
    28981 tatcgggtat tatatttctc cgaaataaac ttcactttac cattcaataa tacattcagt
    29041 acatcttgat caaggtgggt gattttactt acccaatctg ggtcacgcaa catttcaata
    29101 gcttttgacg aaatgttatt tagattccac tcatcaatat ttatcagtaa aaaaccagca
    29161 ttgaaatatc cagaagctaa ctgtggtgta gttaataccg aggctcgatt ttgccaccat
    29221 tcaatatctc tttcagctac aactgcggca atttcattag tagaaaattg ataatctaag
    29281 agttctttaa tactaccctt gcaagcaata tctgcatcaa gatatagtat tttttcatgt
    29341 ttatgataga aataatctgc aattataaat cgaaaatatg ttgcgtaagt ccagtttttc
    29401 gtgcttggta atgacttcag cttatcacaa ttgataacat aaatattaat tcttgaatta
    29461 tattgttttg ctaaatcaga aaaatataat ttgtctttat cactgatata atctgtgaaa
    29521 acatgaaatt cacaagagat ttctctattg tttaatagaa tagatgcgat ggctacacca
    29581 catccaaaaa gaaaatttct gtcaatacca aatgctatat caagagtatt tttagatctt
    29641 atattttgat aatgaaaatt atactcaaaa aggatgatgt cactatcatt gagttgagac
    29701 atatttattc tgtatccaaa attttggcaa aaataagtta cgggttacaa cgattttcga
    29761 atcgttcttt ccctgatttt tgtggctttt gcttctgctt gcgacagcag tccttgttcc
    29821 tgcttgagaa tgtctttaag cggggcggca aaatacacct tcataaagcg ccagatatcc
    29881 cgctgagtca ggccaatatt cattgaagaa aaataaagcc ctataagatc tttatcgcgc
    29941 caacgacctg gaacgcgcgt gcgaagctgc gcccggtgta ggtcaattac cgaaattttt
    30001 aactcctctt ccttaccgga aaaaggcaag tgcagcagga aatgacagat ataacagtca
    30061 cgatggttaa tgcccgcagc atgcatatcg cgcaccatcg tcgcgacacg cttaataagc
    30121 atacgcttta cgcgaacatc tggtgggttg gtcgcccagt cagcacagta atcttccaga
    30181 ctgatggttg gtgtcagatc ttcggtaata ataaacgagg ttctggtcag cggattcatg
    30241 cctttttcgc cgaatgccac cccatacata gtatcaacgc cgacatcccg cagtcgatga
    30301 atcgcattcc attcgcggtc tgcgcctaat actggcatcc gcaatgagag taaatttttg
    30361 attatctctt tcagggtcgt gccgcgatgc catttgagaa aatagctttt tcccgccatt
    30421 tcaaagcgca gagtacggcg agtttccagt tcacgaaata cctcaccctg caaggtttta
    30481 acttcctcaa aaggatcttt gccgcgccat aacgtggcaa acggctcttt aagttcaacc
    30541 atccagacca cccgttatga tatccgccgc tttctctggc agactgtata aatcttgtgt
    30601 atcagcataa tgtcgcgcat tttccgccca agcctggcgc aatgaagatt gcgttaacgc
    30661 tttgcgtaaa atctcattca atgtttcctg gcggaatggc tcagcaatag cctcgccgca
    30721 attagcgtcg acaatataat gcgcatagcc acaaacggca gttgttagta ccggtaatcc
    30781 tgcagtaatc gcttccagca gcacaattcc cgccgcttcc tggtaggcag gatgcagtaa
    30841 taaatccgcc gccgccatta attccgagac atcgttgcgc cccgagaaga agtgaacatt
    30901 actgcgcacg ccgcgttttt ctgccagtgc ctcaaatttt cgcggtttat cctgcccaac
    30961 aacatatagc aatgtgttgt ggcgcagcga atccggtaac gaagcaagtg cttcaatgga
    31021 acgatcgaca cctttacgcg tgaagtctga accgacctgc aacaataaat attgttgttc
    31081 ggttattcca ttcttcttac ggaagatttc acggctattg gctggctgct ggctatattt
    31141 acgatcagga taaatccccg gtggcagaat atgaaaacgc tccgcttcag tctgataatg
    31201 tttctggaaa tcggcgattt gcttatctgt cagcatcagc agctgtgtcg gcttgccctg
    31261 ttcgaaggtt gcccgctcaa aggcggcata atggcgataa cgtgacgtca ggcgatagaa
    31321 aaagcctttt tcctgcgcta ctttctcggc ataacaaaca tcagcggcat aataaacgtc
    31381 cagccccggc attttgttga atccaacgac tttatcgacg ggatgttcgc gtaaatgttt
    31441 ttgcacccag gcaaaatact ccgcattgcg cccgtgattg gtatgcgatt taaccggcac
    31501 tttgatcagt tcaaatacat cagggcattc gccttcccac gactgggtat aaacccgaac
    31561 atgatgacct cgggcggcga ctgtctgagc aatacgcata aaatcgcgct gcaaaccgcc
    31621 aaagggaaaa tatttatata aacaaaaagc aacgatcata acgacgtacc tgtcgtggag
    31681 gagggcagta atttatcgac agcagcaatg acatctgccg ccggaataac cgaaagatac
    31741 atctcatttc ggtcacgctg atcgcgcgtt ggcatttccc ggtaatctcc cgcccagaat
    31801 tgaatcatgt tatttgacca gggacgccag aaaatatgat ctgttgcgcc aaacagcgat
    31861 atcagcggcg tattaactgc agcggcaata tgcgccggtg cggaatcaac gccaataaac
    31921 agctgcgcat gatcgattaa cgcaccaagt tccgggaagg tcacctttcc agccagcgcc
    31981 gttactggtg gcgtctggca tccctgcgca atttcattga cgcaggccag atcgtcttta
    32041 tctgggccgg acgtcagaac gacttcataa ccacgagcat gtaaggcatc aatcacagcg
    32101 gaaaacttgg cgttgtccca gcatttgaag atttgccgcg ccgtaggttg gataaccaca
    32161 taattttgtc caacaccagc gtgatcaagt tcgcgacgca tacgtttcca gcttgcaggc
    32221 gggtaactca tggttgtctg cttcaccaac gaatcaagtc ccaatggtgt cagcacggat
    32281 aagttacttt ccaccacatt tccaccctgc aacggcacca aatgggtgaa acttttacgc
    32341 caaaaagcag actgccgatg atgataatcc tgggaaattt tcacacgagc atttaataag
    32401 cgaaccagta tagcaaccat ccattgatcg gtaagattga cgataaggtc atacttattg
    32461 gcacgtaata ccttgatgag atgaaaaaag ttggcaattt tttctgaggc ttttgctttt
    32521 ttatttttta tgccgtagag cgcgttaatc tctggatttt cagacaggat cgggatggtg
    32581 tcctgataaa gcagcacatc gatttttgcg tcagggtaat tttttttcag agaactaatg
    32641 acgggagtag ttaataacat atccccatga aaacgcatct taatgagcaa aattcttcga
    32701 aatggcttat ccacaagtaa ctctttttgt tgagattatc cgattaaggt gaagcagaaa
    32761 aagcacgcta ccgccccagg ctcaacagct acctgaatac tgataccgca ctgatattga
    32821 cgcgctagtg tatcacttct ttcgcggcaa tccgacccga atgattttta agcacattgt
    32881 tgattttcaa taaagaaatc aatcaaataa aatcattagc aaccatattc tttcactgtt
    32941 gctctgcgat tccttcctgc acgtacagca gaaatcaatg aatcgcataa aaatacaaaa
    33001 aaagtataga tgttcagcaa gaattggcgg gtaaattgct atttaa
//
LOCUS       DAJTYG010000039        32714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTYG010000039 DAJTYG010000000
VERSION     DAJTYG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.039"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            58..1146
                     /locus_tag="OO568_001944"
     CDS             58..1146
                     /locus_tag="OO568_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6641308.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(1234..2532)
                     /gene="exuT"
                     /locus_tag="OO568_001945"
     CDS             complement(1234..2532)
                     /gene="exuT"
                     /locus_tag="OO568_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR6641309.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            3015..4427
                     /gene="uxaC"
                     /locus_tag="OO568_001946"
     CDS             3015..4427
                     /gene="uxaC"
                     /locus_tag="OO568_001946"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6641310.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSSRGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            4442..5929
                     /gene="uxaA"
                     /locus_tag="OO568_001947"
     CDS             4442..5929
                     /gene="uxaA"
                     /locus_tag="OO568_001947"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6641311.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            6012..6563
                     /locus_tag="OO568_001948"
     CDS             6012..6563
                     /locus_tag="OO568_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6641312.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(6568..7812)
                     /gene="sstT"
                     /locus_tag="OO568_001949"
     CDS             complement(6568..7812)
                     /gene="sstT"
                     /locus_tag="OO568_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6641313.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(8408..9373)
                     /gene="alx"
                     /locus_tag="OO568_001950"
     CDS             complement(8408..9373)
                     /gene="alx"
                     /locus_tag="OO568_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6641314.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(9644..10642)
                     /locus_tag="OO568_001951"
     CDS             complement(9644..10642)
                     /locus_tag="OO568_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6641315.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(10721..11413)
                     /locus_tag="OO568_001952"
     CDS             complement(10721..11413)
                     /locus_tag="OO568_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6641316.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(11490..11993)
                     /locus_tag="OO568_001953"
     CDS             complement(11490..11993)
                     /locus_tag="OO568_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6641317.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRMQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            12078..13214
                     /gene="rlmG"
                     /locus_tag="OO568_001954"
     CDS             12078..13214
                     /gene="rlmG"
                     /locus_tag="OO568_001954"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6641318.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            13499..13813
                     /gene="higB"
                     /locus_tag="OO568_001955"
     CDS             13499..13813
                     /gene="higB"
                     /locus_tag="OO568_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6641319.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            13810..14226
                     /gene="higA"
                     /locus_tag="OO568_001956"
     CDS             13810..14226
                     /gene="higA"
                     /locus_tag="OO568_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6641320.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(14271..16289)
                     /gene="fadH"
                     /locus_tag="OO568_001957"
     CDS             complement(14271..16289)
                     /gene="fadH"
                     /locus_tag="OO568_001957"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6641321.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLIGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVHERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLQLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(16515..18866)
                     /gene="ygjK"
                     /locus_tag="OO568_001958"
     CDS             complement(16515..18866)
                     /gene="ygjK"
                     /locus_tag="OO568_001958"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR6641322.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(18883..19302)
                     /locus_tag="OO568_001959"
     CDS             complement(18883..19302)
                     /locus_tag="OO568_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641323.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(19280..19633)
                     /locus_tag="OO568_001960"
     CDS             complement(19280..19633)
                     /locus_tag="OO568_001960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641324.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(19634..19954)
                     /locus_tag="OO568_001961"
     CDS             complement(19634..19954)
                     /locus_tag="OO568_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641325.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(20088..21521)
                     /locus_tag="OO568_001962"
     CDS             complement(20088..21521)
                     /locus_tag="OO568_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6641326.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGDVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(21584..22033)
                     /locus_tag="OO568_001963"
     CDS             complement(21584..22033)
                     /locus_tag="OO568_001963"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6641327.1"
                     /translation="MRIIDNLEQFRQIYASSKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(22030..25116)
                     /gene="ebgA"
                     /locus_tag="OO568_001964"
     CDS             complement(22030..25116)
                     /gene="ebgA"
                     /locus_tag="OO568_001964"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR6641328.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAYYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIIPTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHDNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(25298..26281)
                     /gene="ebgR"
                     /locus_tag="OO568_001965"
     CDS             complement(25298..26281)
                     /gene="ebgR"
                     /locus_tag="OO568_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6641329.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELNN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            26500..26831
                     /locus_tag="OO568_001966"
                     /pseudo
     CDS             26500..26831
                     /locus_tag="OO568_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(26873..28204)
                     /gene="ygjG"
                     /locus_tag="OO568_001967"
     CDS             complement(26873..28204)
                     /gene="ygjG"
                     /locus_tag="OO568_001967"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6641330.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEM
                     FRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            28621..30141
                     /gene="aer"
                     /locus_tag="OO568_001968"
     CDS             28621..30141
                     /gene="aer"
                     /locus_tag="OO568_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR6641331.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMFTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(30295..30918)
                     /gene="nfeR"
                     /locus_tag="OO568_001969"
                     /gene_synonym="yqjI"
     CDS             complement(30295..30918)
                     /gene="nfeR"
                     /locus_tag="OO568_001969"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6641332.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            31195..31941
                     /gene="nfeF"
                     /locus_tag="OO568_001970"
                     /pseudo
     CDS             31195..31941
                     /gene="nfeF"
                     /locus_tag="OO568_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(31995..32070)
                     /locus_tag="OO568_001971"
     tRNA            complement(31995..32070)
                     /locus_tag="OO568_001971"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32035..32037),aa:Ile,seq:cat)
     gene            32195..32701
                     /gene="mug"
                     /locus_tag="OO568_001972"
     CDS             32195..32701
                     /gene="mug"
                     /locus_tag="OO568_001972"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR6641333.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT         7942 a         8496 c         8608 g         7668 t
ORIGIN      
        1 gttgaaacag cctaaattac gtggaaacgt tcagacggcg caaagggagg ttgcaccatg
       61 agaaatcgat tgattgcggc gatattgggc ttgtttggca cgctcactgg cgttcaggca
      121 gctcctgacg tgaccagtga aattacgtat gatttggcat ctggtagagc ggattattac
      181 ttctggaagg atgaagcgtc ggcaggaaat aatggatata tgtggtatga atgttcgtat
      241 cctgacctcc aacaaacctg tacagctaat ggaaatatat cgacagtaca aatctattta
      301 actgaacaac gcagtgggat gcgttggccg gtaaaactca aaggatttaa aacagccatt
      361 gtaagtagcg atgaagcgcc gctaggatgc aaggggggca aagggcttca gacgaatctt
      421 aaggattcta atagatcttc atgtacagaa gatggtcaac attattatat atacgataca
      481 aagtttctta cgctctacct tgagcagaca gagatgaaga atttgccgat tggtggcgtc
      541 tggaagggga aagttaaatt acattcgaac agcccggccc aggactattt cgcaaatatt
      601 accctgaata cgctcgaccc caaccatatt gacgtgttct tcccggagtt cgcccacgcc
      661 acgccaaggg tgcagttaga cttgcatcca acaggaagcg ttaacggcag caactacgcg
      721 caagatctga ccatgttgga catgtgcctg tacgatggtt ttaacggtaa tgccatcagt
      781 tatgaaatca tgctcaaaga tgaagggcga ccagctgcag ggcgcagaga cggttacttc
      841 tctatctatc gtcagggagg gaccaccacc gacgagggag aacgcattga ttaccgggtc
      901 aaaatgtaca acccggaaac cggtgggcaa attgatgtgc gcaataatga aaatatggtc
      961 tggaacagca ttaacctgaa acgtgtgcgt ccggtggtac tgcccggtat tcgctatgcc
     1021 gtgatgtgtg tgccgacacc cttgactctg gcagtcgata aattcagcgt gatggataaa
     1081 caggccgggt actacatggg caaattgtca gtaatcttta cgccttcctt gccaaccatc
     1141 aattaaccct gcaaaccgga caaacgcttt tttctatata aacgacgaag ccgcctgata
     1201 aggcggcttc gtcgttataa cttacgttaa acgttaatgt tgcggtgcgg gatcgttatg
     1261 tgtttcctgc gccacctcaa tcgccggttt gttctgcaat acggtccaga taaccagcgc
     1321 acccagcagg tcaaatactg ccagaactgc gaacagcggg ctgaagccga tggtgtcagc
     1381 cagtgcaccg acaaccagcg caaacagcgt acttgccagc catgcggaca tcccggttaa
     1441 accgttcgcc gtcgccactt cgttacgacc gaacacgtca gaagagagcg taatcagcgc
     1501 gccagacagt gcctggtggg caaaaccacc gatacacagc agcataattg cgacatacgg
     1561 gttggtgaac agaccgatca tacccgggcc aatcatcagc acagcaccca gcgttacgac
     1621 catcttacgg gaaacgatca ggttcacacc aaaccaacgc tggaacagcg gcggcaggta
     1681 accaccgagg atacaaccga ggtcagcaaa cagcatcggc atccaggcga acatcgcgat
     1741 ctctttcagg ttaaagccgt aaactttaaa catgaacagc gggatccacg cgttaaaagt
     1801 accccaggcc ggttctgcta ggaaacgcgg cagtgcgata ccccagaact gacggttacg
     1861 caggatctga ccaacggaca ttttcttcgc cgtgctcacc tggtgctggg cttcctgacc
     1921 attaataata tagtcgcgtt cttcatcggt cagatgcttc tggtcgcgcg gatgtttata
     1981 gaaaatcagc catgccatcg cccagataaa gctcaatgca ccggagatga taaatgccat
     2041 ctgccagctg tgcatcacga ttgcccatac caccagcggc ggcgcaatca tcgcaccaat
     2101 cgaagaacct acgttaaagt agcctactgc gatggaacgc tctttcgccg ggaaccattc
     2161 ggagctggct ttcagacccg ccgggatcat cgctgcttcc gcggcaccga ccgcaccacg
     2221 agcaacagcc aggccacccc agctacctgc cagcgcggtt gcaccacaga acacggccca
     2281 cagcacagca aacattgcat aaccgatttt cgtacccagc acatccagta cataacctgc
     2341 taccggttgc atgaccgtat aagcagcaga ataggctgcg atgatatagg aatactgttg
     2401 ggtggagatg tttaactctt ccatcagagt tggcgcagct gccgccacag tgttacgcgt
     2461 caggtaacca agcacggtgc cgagcgtcac cagtgcgatc atataccaac gtaacccttt
     2521 aattttacgc atgtaaaacc tcatcgtttt gttatatccg caggaagcgc ggccatcatc
     2581 ccgccagagg ctgaagatgt tagttacgac cgggtaaccg aaaaaaaacc ggcacgcccc
     2641 gaacgttgcc atgaaagttt cgtgctaatt cggcttccgt accggttaac ccgattcatc
     2701 taacaaatga atgaatcggc aatttgcatt ccgtcgactt atgatttgcg acggcagaaa
     2761 gataacttgt catacaacct taaaaggtga gagccatcac aaatgtgcga atatttgtag
     2821 ggacattacc tgacgacagc gaggccagta ctggcgcggc ctgcagcgag atttaccact
     2881 ttgagagtaa tttttttaac tacgtttatt gatctaactc acgaaaatat cttcggactc
     2941 tggaaattgg tgtgataact ttgtcagcat cgcaccataa gcaagctagc tcactcgttg
     3001 agaggaagac gaaaatgact ccgtttatga ctgaagattt cctgttagat accgaatttg
     3061 cccgccgtct gtatcacgac tacgcaaaag accagccgat tttcgattac cattgccatt
     3121 tgccgccgca gcagattgcg gaagactatc gttttaaaaa cctgtatgac atctggttga
     3181 agggcgatca ctacaaatgg cgcgctatgc gtaccaacgg tgtggctgag cgtctgtgta
     3241 ctggtgatgc gtctgaccgt gaaaaatttg acgcctgggc ggcgactgtt ccgcacacta
     3301 tcggcaaccc gttataccac tggacgcacc tcgaactgcg tcgtccgttc ggtatcactg
     3361 gcaaattgct ttctccgtca actgccgatg aaatctggaa cgaatgtaac gaactgctgg
     3421 cgcaggataa cttctcctcg cgcggcatca tgcagcagat gaacgtgaaa atggtcggca
     3481 ccaccgatga cccgatcgat tctctggagc atcacgcaga gatcgccaaa gacggctctt
     3541 tcaccatcaa agtgctgccg agctggcgtc cggacaaagc cttcaatatc gaacaggcga
     3601 cctttaacga ctacatggcg aagctgggcg aagtttccga taccgacatt cgccgctttg
     3661 ctgacctgca aactgccttg accaaacgtc tggatcactt cgccgctcac ggctgtaaag
     3721 tgtctgacca tgcgctggac gtggtgatgt ttgctgaagc gaacgaagcg gaactcgaca
     3781 gcattctggc gcgccgtctg gctggcgaaa ccctgagcga gcacgaagtg gcacagttca
     3841 aaactgcggt actggtgttc cttggtgccg aatatgcacg tcgcggctgg gtacagcagt
     3901 accacattgg cgcgctgcgt aataacaacc tgcgtcagtt caaactgctg gggccggatg
     3961 taggctttga ctccatcaac gaccgtccga tggcggaaga gttgtctaag ctgctgagca
     4021 agcagaacga agaaaacctg ctgccgaaaa ccattctcta ctgcctgaac ccgcgcgata
     4081 acgaagtgct gggcaccatg atcggtaact tccagggcga aggtatgccg ggcaaaatgc
     4141 agttcggttc cggctggtgg tttaacgatc agaaagacgg tatggaacgt cagatgaccc
     4201 aactggcgca gctcggtctg ctgagccgtt ttgtcggtat gctgactgac agccgtagct
     4261 tcctgtcata cacccgtcac gaatacttcc gccgcattct gtgccagatg atcggtcgct
     4321 gggtggaagc gggcgaagca ccggcggaca tcaacctgct gggcgagatg gtgaaaaata
     4381 tttgctttaa caatgcgcgt gactacttcg ccattgaact gaactaaggt ctggggttga
     4441 tatgcaatac atcaagatcc atgcgctgga taacgtcgcg gtcgctttag cagatttggc
     4501 tgaaggcaca gaagtcagtg tcgataacca gactgttacg ctgcgccagg atgttgctcg
     4561 tggacataaa tttgcgttaa cggatatcgc aaaaggggcc aacgtcatca aatatggcct
     4621 gccgattggt tatgcattgg cggatattgc ggcgggggaa cacgtccacg cccacaatac
     4681 gcgcacgaat ctgagcgatc tggatcagta tcgctatcaa cctgattttc aggatctgcc
     4741 tgcgcaagcg gcagatcctg aagtgcagat ctatcgtcgc gctaacggcg atgtcggggt
     4801 gcgtaacgag ctgtggatcc tgccaaccgt gggctgtgtc aacggcatcg cgcggcaaat
     4861 ccagaaccgt ttcctgaaag agaccaacaa cgccgaaggt accgacggcg tgttcctctt
     4921 cagccacacc tacggctgct cacaactggg cgacgatcac atcaacaccc gcaccatgct
     4981 gcaaaacatg gtgcgccacc cgaacgcggg cgcagtgctg gtgattggtc tgggctgtga
     5041 aaacaaccag gttgccgcat tccgtgaaac gttgggcgat atcgatcctg aacgcgttca
     5101 tttcatgatc tgccaacagc aggatgatga gatcgaagcc gggatcgagc atttgcatca
     5161 gctgtataac gtgatgcgca acgataaacg cgagccaggc aaactcagcg aactgaagtt
     5221 tggtctggag tgcggtggtt ctgacggtct ttctggtatt actgctaacc cgatgctggg
     5281 gcgtttctct gactacgtga ttgctaacgg cggtactacc gtactgaccg aagtgccgga
     5341 gatgtttggc gcagagcagt tgctgatgga ccattgccgc gacgaagcaa cgtttgaaaa
     5401 actggtcacc atggtcaacg acttcaaaca gtactttatt gcccatgatc agccgatcta
     5461 cgaaaaccca tcaccgggga acaaagcggg cggtatcacc acgctggaag acaaatcact
     5521 tggctgtacc cagaaagcgg gttccagtgt cgtggttgac gtgctgcgtt acggcgagcg
     5581 tctgaaaacg ccagggctga acttgttaag tgcgccgggt aacgatgccg tagcgaccag
     5641 cgccctggcg ggtgcgggct gccatatggt gctgttcagt actggtcgcg gcacgccata
     5701 cggtggattt gtgccgacgg tgaaaatcgc caccaacagt gaactggcgg cgaagaaaaa
     5761 acactggatc gactttgacg cgggtcagct gatccacggt aaagcgatgc cgcagttgct
     5821 ggaagaattt atcgacacca tcgttgagtt tgccaacggt aagcaaacct gcaacgagcg
     5881 taacgacttc cgcgaactgg cgatcttcaa aagcggcgta acgctataaa gtcgaaatga
     5941 gttgttaata aaaaacggcg tttcataatg tgaagcgccg tttgcttttc catacctgct
     6001 aaggatttat catgaccgcg tattggctgg cccagggcgt gggtgtcatc gcctttctga
     6061 ttggtatcac aacatttttc aatcgtgacg aacgtcgctt caaaaagcag ctttcggtct
     6121 atagcgccgt tattggcgta catttttttc ttctgggcac ctatcccgct ggtgccagtg
     6181 ccatccttaa tgccattcgt acattgatta ccttacgcac gcgcagctta tgggtaatgg
     6241 cgatttttat tgtgctgact ggcggaattg gcctcgcgaa gttccatcat cctgtcgaac
     6301 tattgccggt tatcggtacg attgtcagta cctgggcgct gttccgctgt aaagggctca
     6361 ccatgcgctg cgtgatgtgg ttttcaacgt gttgctgggt gattcacaac ttctgggcgg
     6421 ggtcgatagg cggcacgatg attgagggta gttttctgct tatgaatggc ctgaatatca
     6481 ttcgtttctg gcggatgcag aaaaggggaa ttgatccgtt taaagtagag aaaacccctc
     6541 ccgccataga cgaaaggggt taaacaatta attacgcagg gcgctatttg ccagacgatc
     6601 gtcttccgcc tggcaagctg ccgcagtgaa cagcacgtca gttgaagagt tcagcgcggt
     6661 ttcgcaagag tcctgcaata cgccgataat aaagccgacg gcaaccacct gcatggcgat
     6721 atcgttcgaa ataccgaaca tattacaggc cagtgggatc agcagcaaag aaccccccgc
     6781 cacgccggat gcgccacagg cacacagaga agccactacg ctcaacagca gcgctgtggg
     6841 cagatcgacc ggaataccca gcgtattaac cgcagccagc gtcaacacgg taatggtgat
     6901 tgctgcgccc gccatattga tggtggctcc cagcggaata gaaactgaat aggtatcgcg
     6961 atccagattc agcttttcac acagcgccat attcaccgga atgttcgctg cagagctgcg
     7021 ggtgaagaag gcatacacac cgctttcgcg caggcacagc agcaccagcg ggaacgggtt
     7081 acgacgaatt ttccaccaca ccagcaatgg gttaaccacc agcgccacca gtaacataca
     7141 gccaaccagc acgaccagca gttgcgcgta gccccacagt gtggagaaac cggtggttgc
     7201 cagggtagaa gaaaccagcc caaaaatacc gatcggtgcg aagcgaatga ccagtttcac
     7261 cataaaggta acggcattcg acatatcatt gaccaggttt ttggtggtct cgttaccgtg
     7321 acgcagtgcg aagccgaggc cgatcgccca caccagaatc ccgatgtagt tacctttcag
     7381 cagggcgtcg atggggttgg aaaccatgct cattaccagt ccgcgcatca cttcgacaat
     7441 gcctgacggc ggcgaaatat cacccgcgct actggacaag tgcagggtag aagggaaggc
     7501 aaagctgaag actactgcgg ccagagctgc agagaaggtg cccagcagat agaggaacaa
     7561 aataggacgg atattggttt tctgcccgtg ctggtggtta gcaatagatg ccattaccag
     7621 catcaacacc aggatggggg caacagcttt cagtgcgccg acgaacaaag tacctaacag
     7681 accaacagct tccgccgcgg gttttgagat ccatgccaga agaatcccca gaacaaggcc
     7741 gaccaggatt tgttttacca ggctgccatg agccagacgc cggaatagcc ccggtgaacg
     7801 ttgcgtagtc atttttcgat cctttcattg tgttgtcgca catccctggt gcgttctgtc
     7861 gacgcatctt tcaggatgta acaaagtgtt tgcaaggtcg agtataagga aagttacagc
     7921 ttcgggaagc gaaaaatgct ggattttaaa gagaggtcat gttttgcaac ttaatggttg
     7981 catttattta acataaacag agggaaggac tggctattaa ggctgattag ataacatatg
     8041 ggcaaaacta tttggaatat gagaacggtt gcgctccttt aacgtgttat gccatataaa
     8101 gtcactggcg cagggagcgc gcagggggcg gccaatcgcc gccgccccct gcacccccag
     8161 gctctggcga acaaaatcgc cgctgcgcgg ttccctcagc ctatcccttg cggctaccgg
     8221 gtcgggcgca aggtaacgtc cctgtaagat gcgccctcgc cccggcctcc agggaacgct
     8281 tcggcgattt tgacgccacc aacacccgtg cggtcttttt gtaaagagct ataagccgat
     8341 tacataatta atagctcacc tttgtctgga atgagttatc tctaggaatg gcttaatata
     8401 cagcgaatca ttctccccgc tgcttatcat gccgataatt cacccaggcg ttgataataa
     8461 acgtcatcac cagaatgcca aacaccacgc ccagcgagac ggcgattgga atatggtaga
     8521 agtcgacaat cagcatcttg ataccgataa acaccagaat caccgccagg ccatatttga
     8581 gcatcgagaa acgctctgct acgcccgcca gcaggaaata catcgcacgc aggccgagga
     8641 ttgcaaacag gtttgaggtc agcacaatga acgggtcagt ggtcacggcg aagatagccg
     8701 gaatgctatc cacggcaaaa atcacgtcgc tcaattccac cagaatcagt accagcatca
     8761 gcggtgtggc gtacagcaat ccattcttac gcacaaagaa atgctcgttg tcgatggtgt
     8821 ctgtcatgcg caaatgaccg cgtagccagc gcaccagcgg cttgtcgcca atgcctgatt
     8881 catcttcatg ggcgagcgcc atcttcacgc cggtaaacag caggaaggca ccgaagatat
     8941 acaggatcca gtcgaactgc gaaatcagcc agctgcctgt gaagatcatg atggtacgca
     9001 gaacaatcgc cccgagcacg ccatacacca gcacgcgacg ttgtaatgcc gccggaacgg
     9061 agaaatagct gaacaacatc agccagacaa agacgttatc gaccgccagc gatttctcaa
     9121 tcagataacc cgtgagaaag gccagggcct gtggatcggc aacggcgcga ccttcggttt
     9181 gcaccagata ccaccagaaa gcggcgttaa acagtaacga cagcgtcacc cagaccagcg
     9241 accaggccgc agcctgtttc atggtcatgg catgtgcccc acgacgcccc tgcaacaaca
     9301 ggtcgatagc cagcataatg gcgacaacaa cagcgaatcc gccccatagc aacggcgtgc
     9361 cgacagtatt catagaagtt ccttacacat aaaaaaacgg ccaacgtcgg aagacgtcag
     9421 ccgctgcttt ttatgcatag acctcgcctt ccggcaaggt ctcacttaca acaaaaaagg
     9481 aatacgtctg cgtattcctt tcgggttgcc cggtgaccgg atgtggtttt tcacacatcg
     9541 taatgacgat cgaccggcaa tgaagttact cccctttgca ggtaacaaag tatgacagac
     9601 cattcagtca gtcaatggtc tgttacatac cttttacact gttttacttt ggcaagtata
     9661 gttttacgct atctgccgga aaaatcaccc cagtctggcg gcggatctcg gtcagcagtt
     9721 tggctgtgat gcgactgacc gccagccctg gatgatccac cagatgctca tccaccaggg
     9781 tagcgaacag ctctgcttcg tagagcatgg tattaatatg ctgcggctgg gtgagatcct
     9841 gcatctggct gccacgcggc acaaagcaga ctttctggca ttcagacagt ttttcaatca
     9901 ccagcgatcc cgcttcgccc tgaatctcgc ttcccaggac agaatcactg acctttgagt
     9961 gctgcaaggt gacgctgaaa tcaccgtaat ccatcaccac cacgccgtgg gcgtcaacgc
    10021 cgcttgccag caaactcgcc gttgcctgca cgcttttcgg ctcaccaaat aacgccaccg
    10081 ccgacgccag gcagtaaaag ccgatatcca taattgaacc gttagagaat gccggattaa
    10141 aggtgttggg attctcaccg tccaggtaac gttgataccg cgaggaatac tggcaatagt
    10201 tgaaaaagac tttacgcagt ttgcctgctt tcggcagcgc ctggcgcaac aaatgaaagt
    10261 tcggcaggca ggcggtttta aatgcctcaa acagcaccac ctgattttcg cgcgcacagg
    10321 caatggcggc atccacttcc gccagattcg acgccagcgg tttttcgcaa atcacatgaa
    10381 ttttatggct aaggaaaagt tgtgtctggg aaaagtgcag ggaattcggg ctggcaatat
    10441 acaccgcgtc aatggcatcg ctttccgcca tcgcttccag cgacgtaaac agatgctcga
    10501 cagaaaaatc attggcgaag tgctgagcct gctcaaggct gcgggaatat acggcggtta
    10561 acttgtattt accgctctca tgggcggcct cgacgaactg gcgagtgatc cagttcgtac
    10621 caatcacagc gaaacgtatc ataacgcgtg gatactccat aaagggattc tgtcgtcaat
    10681 gtagcacgcc tttttcatct tcgacgtgcg cagatacgca ttattctttc gccggacagc
    10741 catgctcact aaacggccca atcatgaccg atggcccgag atatttcggc tggcgatgga
    10801 ggatttttgc gtcaatcacc gcgctgacac gagcgagttt ctcccgcagt tgagtataaa
    10861 aaccatgctt catattgagg tctggcgcgt taaacccgat agcgtcgatg ccgtactgtt
    10921 ttgccagcca gatggcgcgt tggttatgaa attcttgcga gataatggtg atatggtttt
    10981 ccccaaacac ctttttggca cgcactaccg aatccagcgt tgagaatccg gcatagtcgc
    11041 aaaagatcac tttcgccggt acgcctttag cgatcaaagc ctgctgcata cctgatgctt
    11101 catcgtaatt tttacgtccg ttatcgccac tcaccagtag ccatttcact tttccggcgt
    11161 ggtacaacgc tgctgcggta tcaatacgtc gggtaaagta gcgattaccc ggcctcgccc
    11221 ccagcaataa acccacgtta cgcgccggga cggcgttgac atcgccccag gtcagctgct
    11281 tgctggcgtt gaccataacg cgatcggcga taaaaatggt cgctcctgcc acaagtaaca
    11341 gtaagcaggc aatctttaga gtccggcgag aaaaacaaat acggagaaga aggcgggcaa
    11401 atgcgcgcag catggtcgtt atccttaacg aaagggggga ggtcgatttt aggcgctaaa
    11461 gaaagaaaag ataacgcctg acatcatttt cagattttgt cctgcccaag cgacaactgc
    11521 gttagccaca gccgggtgtc gaactcaagc tggtggtact gcggctccat atggcagcac
    11581 aattgataga acgctttgtt atgctctttc tcctttaggt gcgccagctc gtgcaccacg
    11641 atcatgcgca aaaacggttc cggcgcgttg cgaaacacgg tagcgacgcg gatctctgct
    11701 tttgctttca gcttgccgcc ctgcatacga gaaacagcgg tatgtaaccc gagcgcgttt
    11761 tttagcacat ggatcttatt gtcatacatc actttattga tcggcggggc attacgcaga
    11821 aactgatttt tcagatcctg agtatattgc cagagagctt tatcggtggc gtagtcgtgt
    11881 gttcccggat agcgttttgc cagcacatcg cccagacgct gttcgttaat cagtgttcgc
    11941 acctgcgaaa gcagctgctc gggataaccc tgaagataag taagattgct cataaccgcc
    12001 cacgaaaaag agaaaaaggg tatactcacg cacccttttc aggggcaacg tcgaaatttt
    12061 atcattcagg agggccgatg agccacttag acaacggttt ccgttcactg acactacaac
    12121 gttttccggc gacggatgac gttaacccgc tacaggcgtg ggaagcggca gatgaatatt
    12181 tgctgcaaca gttggacgac acagaaatcc ccggcccggt gttgatcctg aatgatgcct
    12241 ttggtgcgtt aagctgcgcg ctggcggaac ataagccgta cagcattggc gactcataca
    12301 tcagtgaact ggcgacgcgc gagaatttac gcctaaacgg gattgatgag tcgagcgtga
    12361 agtttctcga cagcaccgcc gactacccgc aacagccggg tgtggtgctg atcaaagtgc
    12421 cgaaaacact ggcattgctg gaacagcaac tgcgtgcgct gcgcaaagtg gtcacgtcgg
    12481 atacacgtat tattgccggt gccaaagccc gtgacattca cacttccacg ctggaactgt
    12541 tcgaaaaagt gctcggcccg accaccacca cgctggcatg gaaaaaagcg cgcctgatta
    12601 attgcacgtt caatgaaccg ccgctggccg atgcgccgca gaccgttagc tggaagctgg
    12661 aaggtactga ctggactatc cataaccatg cgaatgtctt ctcccgcacc gggcttgata
    12721 tcggcgcgcg cttctttatg caacatctgc cagagaatct cgaaggggag attgtcgatc
    12781 tcggttgcgg taatggcgtt attggtctga cgctgcttga taaaaacccg caggcgaaag
    12841 tggtgtttgt cgatgaatcg ccgatggcgg ttgcttccag ccgtttgaac gttgaaacca
    12901 acatgccaga ggcgttggat cgcagcgagt ttatgatcaa caacgcgctc tccggcgtgg
    12961 agcctttccg ctttaatgct gtgctctgca accctccgtt tcaccaacaa catgcgctga
    13021 ccgataacgt cgcctgggag atgttccacc acgcccgccg ctgcctgaaa atcaacggcg
    13081 agctgtatat cgttgccaac cgccatctgg attacttcca taaactgaag aaaattttcg
    13141 gcaactgcac cactattgcg acgaataata aatttgtggt gctgaaagcg gtgaagctgg
    13201 gacgtcgtcg gtaagattgg gagttacctg ccggatgcgg cgtaaacgcc ttatccggcc
    13261 tacgttcagc acgttacccc caggccggat aagacgcggc aagcgtcgca tccggcaatg
    13321 tctgctcagt tctggaatcg cgcttccata aaacaaaaaa tccctgtcac catcctctac
    13381 attctcttgt ttagcgtttt tctacgttta ttcttccgtc acacagataa attcatccgt
    13441 tgccaatctg tcaacggatg ttatcatatt gccaatttgt caacggacgt gatgaagaat
    13501 gcacctgata actcaaaaag cattgaaaga tgctgcggaa aaatacccgc aacataaaac
    13561 ggagttggtg gctctgggga acacgattgc taagggatat ttcaaaaaac ctgagtcatt
    13621 aaaagcagta ttcccatctc tggataactt caaatatctg gataagcatt atgttttcaa
    13681 tgttgggggc aatgaattac gtgttgtagc aatggtcttt tttgaatcgc aaaagtgcta
    13741 catacgtgaa gttatgacgc ataaagaata cgatttcttt accgctgttc atcgtactaa
    13801 ggggaaaaaa tgattgcgat tgccgacatc ttgcaagcag gagaaaagct aactgctgtg
    13861 gcaccttttc tggcgggtat tcagaacgag gaacaataca cccaggcgct ggaactggta
    13921 gatcatctgc tgctcaacga tcctgaaaac cccttgctgg atctggtgtg tgccaaaata
    13981 accgcgtggg aagaatcagc gcccgaattt gcggaattta atgccatggc tcaagccatg
    14041 cctggcggta tcgccgtgat tcgtaccctt atggatcaat atggtttaac cctttccgat
    14101 ctgccggaaa ttggcagtaa atctatggtg tcacgcgttt tgagcgggaa gaggaaatta
    14161 acgctggaac acgctaaaaa attggcaacg cgattcggca tttctcccgc cttgtttatt
    14221 gattaacgcg acgtgccgga tgcgttgcat ccggcgcttc aaataacgat ttaaatctcc
    14281 agcgccagcc gtgttccctg ggcaattgcc cgtcgtgcgt ccagctccat agccacatcg
    14341 cagccgccaa ttaaatgcac agttttcccg ctatcaatca gcggttgcgc cagcgcgcgg
    14401 tttggctctt gccctgcgca gatcaccaca ttgtccactg ctaatacctg cgtttcgccg
    14461 ttgatcacca catgcagccc gtcatcgtca atcttctgat aacttacgcc tgggatcatt
    14521 ttcacacccc gcgagagcag ggtggtgcga tggatccagc cggtggtttt gcctaacccc
    14581 tgtcccggtt tgctggcttt gcgctggagc atcacaattt gccgtgggct acgcgggatc
    14641 tgcattccct gcgggcttaa gccaccagcc tgttgtaggc tgctgtcgat cccccactca
    14701 ttacagaacc tggcgatatt ctggctggtg gattcgcccg gctgacttaa atacatcgcc
    14761 gtatcaaaac caatcccgcc acaaccgatg atggcaacct tgttgcctac cggcgctttg
    14821 tcgcgcagta catcgagata actcaatacc ttcggatgat cgatcccgtc gataggcgga
    14881 acacgcggca cgatcccact ggcgaggatc gtttcatcga acgcctgtaa ctgatccgcc
    14941 gtcacggtgt gattgagttt cagcgtcacg cccgtcactt cgatcatccg gcggtagtag
    15001 cgcagcgttt cgtaaaactc ctctttgccg gggatctgtt tggcgatatt aaactgcccg
    15061 ccgatctcgc tgtgcgcatc aaacaatgtt acctgatgcc cacgcgccgc cgcgttaatg
    15121 gcaaacgcca gcccagcagg tccagcgccg accaccgcca gatttttttt ctgcacggcg
    15181 ggaaggattg gcattttggt ttcgtggcag gcgcgaggat tcaccaggca cgaggtgact
    15241 ttgccaacga agatctgatc gaggcaggcc tggttgcagc caatacaagt gttgatctca
    15301 tcggctcgcc ctgattgcgc ttttgacagc agttccgcat ccgcgagaaa cggtcgcgcc
    15361 atcgatacta tatcggcatc gccgcgcgag agaatatcgt cggcaacctg cggatcgtta
    15421 atccggttgg tggttaccag ctgcagcgag acgtggcctt tcagtttgcg cgtgacccag
    15481 ctaaatgcgc cgcgcggcac gggcgtggcg atggtcggaa tccgcgcttc gtgccagcct
    15541 attccggtgt tgataatggt cgcgcccgcc gcttcaatgg cctgcgccag ttctaccgtt
    15601 tcggcaaaag tgccgccgcc ttcgaccagg tcgagcatcg acaaccggta aataataatg
    15661 aagtcgttgc cgacgcgttc gtgcaccgta cgcactactt ctacggcaaa tcgcatccgg
    15721 ttgcggtaat cgccgcccca ctggtcacta cgctgattgg tgcgcagcgt cagaaattcg
    15781 ttgatcaaat acccttcaga gcccatcact tcgacgccat cgtatccggc ctcccgcgcc
    15841 agttgcgcgc agcgggcgaa atcgtcgatc agttgcagga tctcttcatg ggtgagttcg
    15901 tgaggaacga aacggttgat gggggcctgc aatgcggacg gtgcgaccag atgcggttgg
    15961 tagctgtagc gcccggtatg caaaatttgc agggcgattt tgccgccttc ctgatgtacg
    16021 gcttcggtaa tggtgcgatg gtgtgggatc tggctggcgt cgttgagcat tgcgccacct
    16081 tccatgccaa cgcctattaa atctggtgca atgccgccgc tgacaatcag cgccacgccg
    16141 tgacgggcgc gttcggcata aaacgcagcc agccgttcag caccgtccgg gtattcctcc
    16201 agcccggtgt gcattgaacc catcaacacg cggtttttta acgtggtaaa acctaaatcc
    16261 agcggggcga acagcgacgg gtagctcata aaattgtcca gtatgtaaaa taattgttat
    16321 gtggtcggat gagttctaat ttagccgtcg atggagaaaa gggaaaaggg gagtgcgggg
    16381 tttgtgatgg gattcaaaaa gaatacgccc ggtggtaacg ccggatgcga cgcttgccgt
    16441 gttttatccg gcttggggga ttgcacatgt agggcggata aggcgttcac gccgcatccg
    16501 ccagccgttg caaattactg cttacggaaa aaatcgttat acaacatata caaatgagcg
    16561 gcactccagg agaaatttgg tgcgccttgc tgcgcgcctg tcagcgggtt gtaattctcc
    16621 tgaattgggc catcggcggt taatcctttg gcgtgcctga agaacgtatc cgccagcttc
    16681 agggcatcat cacgataacc gtaacgctcc atccctttca gaccaaacca gaactgatcc
    16741 acccatacgc gcccgcgcca gtagatatca gcgccaaaag ccggattggt taacgccgcc
    16801 gttcccagcg gaacaaaggt gttgaactct ttaggatcga gcatcacctt caccaccgcg
    16861 tcggcattgg cctgcgttgc cgcaccgtta aacagcggcg accagccttc cggcccttta
    16921 ccgcgctcaa caatcggttt gcccgcgcag ccgttcgcca gtggtttatc ttcaatacgt
    16981 acgtcatagt agaactgggt agtcgggtcg aacatacagg tgttgatgta gtccgcgagc
    17041 tgctgtgcca actggcgata gcgtttggcc tcttctggct taccgaggat ggttgccatc
    17101 tccgccagat aatggttatc gctgtacata tagctggcct gatccaccga ctcctgcaat
    17161 agcgagtagc ccagcaacgt tccgtcctga ctgcggtttt cggcgaattt caccgtccag
    17221 tcgctacgtt tgccgccatt agcgacatat ttatccaact gttctttgtc gataaaacca
    17281 aagacggcgg catcatcacg ccccgattcc cacgacgcag cgacctgtgc cggaatttcc
    17341 agactgtcgt actggccttt ctccaccacg cgggcgtagt tgttcagccc agactgcgtc
    17401 tcttctttat cgcctttttt caccgtaaac agcatctcgc cgctctcggt gttgtgggct
    17461 ttgtcgcggg tcgcgccata ttccggcacc ccgttgccgt tatgatcgcg gttacgtaac
    17521 caccagtcgt gataggccac cagtttcggg tacatctctg ccaaccaggc tttatcctgg
    17581 gtgacgttgt atacttccat caccgaccag gcggcaaggc tgggtttggt attacgttcg
    17641 ttccagttgc cgccatcgcc accacgctcg gggctaaggt tccacgctat caggtcgggg
    17701 acaaagccca catcctgcgg acgcacgctg tcgccaggct ggatctgcca ggagaagacc
    17761 gcgcggatat tctctttggc gatatccgga ttgaaatgcg ccatcgcaaa cgcctgcttc
    17821 caggtatccc acggccaggt ctgattgccg gagaaccagc gtccggtcac cgacggcgtg
    17881 acggtgttat aattcaccgc accgcccggt gagcgccagt taccgttgag cgtttcgatc
    17941 gctttcaccg cgacgcgcgt ctgttccggc gtcgcatccg gattggttaa ccctttcttc
    18001 aggtattctt cccagcgttg ctgcgaggcg gtgagataaa acgccggacg cgccagaata
    18061 tcgcggatct gcatttgctc tttgctaact tcctgagcgg tcagcagatg ggaataggtg
    18121 gtgtagagcg tggtcgaacc gttgatatgc gccttactgg taaagcgatt accgttgatt
    18181 tcagtctgca ccggcaggga tttatgcacc tgatattccg attcgccgga ggtcagcaga
    18241 tcccaggtgg cgcgcacttt gccaaaggtg actttcaggc catcacgggt ggcgctgatt
    18301 ttgcgctgat agtcagggta ttcgccagca atggttttat cggaaagcgg tttcccttct
    18361 ttcgcttcca gtttttccag cagttcgcca tcccacacca gatccagcgg tttattgctg
    18421 atgattttag tttccagtag tgacgtgcgc ggcgtggcga agcgcagagt catttcgacc
    18481 tgcacatctt ttgctgtcag tttttgcacc agcgcgccgg gaatactgta tgcctccagc
    18541 gtgaagttga ctttcttgcc gtcttgccag acagtcaggc ggtcgaaatt gctggccata
    18601 aagttgatgt attcttccgt cagcagcgca acgcccggaa agccgcccat ggtgttcggg
    18661 ccgtctggca acagatgacc atgccaggct ccgagatcga aaaacggatt aaaacgctgg
    18721 tgatcgtcgt aatcgtaatc cttcatgtac tgcggcgcac cagtacggtt aatcacgttt
    18781 ttataattgt cggcgttagc ggcatgagcg gaaaaactta tcagcagagc gcaggttact
    18841 ggcgttaaaa tagttttgat tttcatgaaa ttacagtcct tattaccagt agaaatattg
    18901 catcaggaag atttggtcgc tgggatcgtt agaggcattt ttcatgtaga agcggctatc
    18961 aaaagccgtg gcgaaacgtc cgccgccata ttcgtaccag ataccaaact gcatgaaaga
    19021 ggtggtttca tcgtcagcgt ggtcagggcg atgtttggcg taggtgtaag cggtggagag
    19081 ataaacgccc tttaacgctt catacatggc gctcaccatc atcccgcttt cacttcccgg
    19141 tacgtccagc ccgtcggcac gtccggtatg gtgccaggcg cgagcggcaa agttaggggt
    19201 aaacaggccc gtgagataga gttgtcctgt gccgtcggta atttccagac cattcatcca
    19261 ggtgatgcct tcggcaaggt catactggat gtaaccgttc atccattgcc ggataggtgt
    19321 atttgtcgtc gtagcgatcg tacttgccaa aatgtagcca ggctttgctt tcgtcatgat
    19381 gtccggcagg tgtggcggtg acgctataac gaaagttgcc gcttaagttc tgtaccttca
    19441 gcgcggtcat aatgtcgcgg gtattgggaa tgacgtagcc caaatcgggt gtgaagtcgc
    19501 cccaccattg caggtcatcc agcgaggaat cctgacgcag actgagcatg atttcggtgc
    19561 catcattggt acgataaccg ccatagaaac ggttaatccc accttcgaaa ccaccccagc
    19621 tatggtcagg cacctaggca ttaccgtcgt ggtcggcctg cacggtccag ccttcgccgt
    19681 aaatcaggcc gaaccaggag ccgtacttca tctccaggcc accttcaata taggtgccgt
    19741 cgctgtattt atgtttattc tcaccctcta aatccatata tccaccaagc cctaattcgc
    19801 cgtaaaaacg aaatgaagga gaagatgtcg aggtaattgc gggtgttttt gtgtcgtcat
    19861 gttctgccgg acgtgcttct tctcctgccg cagaaaaggc gtagcaaaac ggcagagcaa
    19921 gtaatgctct gcatggtgca gtgattaatt tcattgtgtt aatccatgag ctaaagaaaa
    19981 agtacagcct tttatcgttg taattataaa aatataaagg cagaaactat tttccctctc
    20041 ctcatccagg aagagggaaa atgcctgctc attttttatt agtctgctta atcaacattg
    20101 ctggctggcg tcgcttcaat atggcgctct tcagccgtta atccctttat atatttactg
    20161 tatttccacc acgcaaagcc gaggaagata acaataccgc cgacgttata aaaaatgatg
    20221 gtcagaatat tcgcgccagt cgggaatgtc gaagcgacaa accctacggc aaatatcgca
    20281 atcagcattg aaacaacgat aataccggtg cggcgggagc ccatacggaa atcgcgtggc
    20341 aggtgatcta atttggcgcg taaattcagg taagccagca tgatgaataa cggcggaagc
    20401 atggacgctg cggcggtcat attaataatg gtattcatca gatcctgcac ggtattggaa
    20461 ccgagcatcg ggataatcat cagcgggatg acgatcagaa actggatcca tgctgcgcgc
    20521 gccggaacgc cgttttcgtt aagttcgacg gttttcttac caaagatgcc ttccgggatt
    20581 tcggagaaga aaattttcac cggcgttgcg gtccacatca gcagggaacc gaacattgcg
    20641 gtaaaggaca ccagaccgac aaagcgattc atcaacgctt ccggcagacc aaaatacgcc
    20701 gccatgccgt ggaatacctg caccgatccg ccggtaaatt tcaactcttt gctgctgacg
    20761 aagacgttaa tcagcaccga ggagacggaa tacagtacgc cgataaaaat cccggcgagg
    20821 atgatcactt taacgaacga tttcgaaccg cctttgacgt cgttaacgta caccgcgacg
    20881 gactccgcgc cacctgcggc cataaagatc caggtggtaa cgccgaggaa cgcccagttg
    20941 aagttcggga tcatcgcgtc aacggtgatc ggatcggcag gctgtacgtc gccaaccagt
    21001 gccgtacctg ccagtaaaat gtaggagagc gttaacagca gcatcagcgt tgaagtgacg
    21061 gaggtaattg gccccagcat tttcgccccg ttggtggaaa cccaggtgga gaaggcgaac
    21121 agcaccatac tgataacggt ggtggcaacc ggcgtcataa tatattcgta gccgaggaag
    21181 gcatacgaag cataagcaat aacgcgcggc aacagcgagg taaaaaagaa cagatttacg
    21241 aaccagtaag tgtaggcagt aataaatgcc caacgtccgc ccagcgaact ttttacccac
    21301 gcgtagacac cggcttctga gtttttattt aacgaaacaa attctgcgat gatcagacag
    21361 aagggaataa aataaaaaat cgtcgcgagg aaaaacatcg gtgccgaggc cagtccaagc
    21421 tcaatattat tattgataac gttgttaaag ctgtaaacgg cggcaaaagt cagcgagagc
    21481 aagccgaatt tgccgattgt attacgtttg gtatcagaca taacacatct ccgaaaatgt
    21541 gtagggtagc ggtggcggca aactcctttt gccgccgtag ttgttatttg ttatggaaat
    21601 aaccatcttc gatggtgact ttgagaacca cttttttgac cgcgttattg cagataaacc
    21661 gatacgcttc atggatatcg caaataacga tttgcccttc gtggacctct acggtttctc
    21721 cgcagccttt taaatattca cggtcagttt catcacgata atattccact acctgtaatg
    21781 tctctttcgg cgcatattca attttttgct gcccttgcag gtagtaatgc acttcaaaat
    21841 agcgtcgatg cccggtaaat agcgcatcgg tcgcggagtc attttccaca cggtaggtca
    21901 atgaatcacc gatggagtgg gcgacgccag gctgaatgtt gtcgatattt tcaatcgctt
    21961 caacgcagcg ttgccacttc ttgctagagg cgtaaatctg gcggaactgt tctaagttat
    22021 cgatgatcct cattgcttat tctcgctgtg caaattcgtg gaaaagaacc ctgcgccgaa
    22081 ctcatacgac gccaggcttt gcgcggtagc ttctccgcca gaaaccggca gcaacgtaaa
    22141 gccgtagctg aagtcacgga accagacgcg ccaggagtcc agcacctcgc tgccccagga
    22201 gttggagccg aggccaagta gctggtggtc gaggttcagg gtgatgtcat cactgcgctg
    22261 cagctcgtta cagtgctggg cagcgtggat gttttcctgg gtatagtgcc aggcgctgaa
    22321 gttaattggg cgctgcggaa ccaccagcag accgttgtcg tggcggttag tcagtgccgt
    22381 ccagcggaca tgctgacggt tgccgttgtt ctgcgggaag ggatagttct cgaacatggc
    22441 atcgacggtg ctgcgccaga tatcgatgat gttagcctgc tggctgtcgg cgtagttttc
    22501 gcccggtcca cgaccgtaat acgccacctg atcgtattcg ccgttaatcc ccatggtgaa
    22561 accgatgcac ggaatgatgt gcggatagtc gccgtaacgc tcgccggaaa gcgccacgtt
    22621 aacctggcca tcggcagcga tgcgccagat gtaggtacag cgcatcccga agtcaaacac
    22681 cggcggtgca ataactgtgc ggctgatgat caacacttca ccatcgctct gttctacggc
    22741 aaagtcgcgc agatgttcct gcatgatctg caaatgattc ggctgccaca acccttcgta
    22801 ctcctgcttg tggttgtcga tcatcggctt gaagaagttg atctttggct cgcgggtcag
    22861 cagcgactcg ccatttacct gccaggatgt cggtttgcca ctcatttttg agaaggtgat
    22921 cgcgaagttg tagccgcgaa cggtgcagct caaacgatcg tcttccagcg tcagcggacg
    22981 cgcattattt ggtgcgaaag gcactggctg cgcggtgttt tccttcagcg ggaactgata
    23041 agtggcgata gaatgcccgg cttcgctgta gcgggtgcgg gaatctttgg tcaccgtaat
    23101 gttgaggaac gcttcgcggg cgtccagctg cggcagcgtg atctgcaagg gggcttcgct
    23161 gttcggcgca acgtcgcgca gtttaatctg ctgcgtcgcg agcgtttcac cttcggcgcg
    23221 cacctctgcg tgcagggtgt agtcatcaag cgtggtaaac cacagtttat tttcgacttt
    23281 caattcgccg cgagtcagcg cgtggatttt taccggcgcg ataacctgtt tgtactcttt
    23341 caggcccgat cccggcgtct gatcggaata gatcaaacca tcaagacaga agttatagtt
    23401 gttgggatag tcgccgtagt cgccgccgaa tttataccag acattgccgt ggtcgtcctg
    23461 tgcctggatc ccgtggtcgc accactccca gacataatga ccctgaatgc aatcgtgctt
    23521 atagaagacg ttctggtact ccgtcagccc gcccggtccg ttccccatcg catgagcata
    23581 ttcacagatg atgcgcggct tcggatgcgg gtattcacca aactcattca tcagcggcac
    23641 gcgggtgtac atggtgggaa taatatcgac cacttcagca tcgcgatctt cttcgtaatg
    23701 caccagtcgc gtgtcatcca gcgctttcgc cgcatggtac atcgcgcgga tgttacagcc
    23761 atagccggat tcattgccca gcgaccagat gatgatcgac ggatggtttt tctgtgcgtg
    23821 gatatggcga acaatgcgct cgacgtagac tttttcccac tgcggatcgt cggtaatacg
    23881 gctgatatcg ccgacattag caaagccgtg cgattcgacg tcggtttccg ccatcacaaa
    23941 caggccgtag atatcacaca attcgtaaaa acgcggatcg ttcgggtagt aagcggtacg
    24001 cacggagttg atattgtgct gcttcatcaa ctggagatct ttctcgacgc gatccattcc
    24061 aacggcgcgg cctttgcgat gatcgttgtc gtgacggttg acgccgtgca gcatcacata
    24121 acggttattg atccagaaca gaccgtcgcg cactttgata tcacggaagc caacgcgttg
    24181 tggcaccact tccagaacgt tgccgttggc gtctttcagc gtcatgacca gatggtaaag
    24241 ataaggggat tctgctgacc attgctgcgg ctgttcgaca gtaaaagcaa agctggcgct
    24301 ggtcagtttt tcaattgcca aatgatcaat ggcgctgctg tgcaccacgc gttcgccatc
    24361 aaacagggta tattccagcg tcgtgacgac aggggaggcg gcgagatttt ccagcaccac
    24421 ttcgcaggaa agcgtggcat cgcaataggc ttcgtcaaag tcggtacgca cagtgaaatc
    24481 gttaatatgc gttaggtgtt ttccgaccag ataaacatcg cggaagatcc ccgccgacca
    24541 ccacatatcc tggtcttcca cgtaggtaga gtccgcccac tgcatcacgc gcacacacaa
    24601 caggttgtcg ccggttttaa ccatcgcgct gatgtcaaac tctgcggtca ggcgactgcc
    24661 cttgctgaaa cccacatact gaccgttaac gtagacttca aaataggttt cgacgccgtc
    24721 aaatttaatc agcgtctgtt taccctgcca gccgtcgctg agggtgaaaa tacgttgata
    24781 ggcaccggtt gggttatcgc tggggacaaa cggcacatcg atggggaacg gaaaaccttc
    24841 gtcggtatat tgcagtttgc cgtgaccttc catttgccac atggcgggga cggtaatatg
    24901 cccccagtca gccattaact cagaggtgaa ggcttctggt acttgcagcg gatggtcaaa
    24961 aaagtggaaa ttccactgac cgcttaaggg cagaaacagg ctgctggttt cgcgggcaaa
    25021 ggtacgcgct tgcgcaacag aatcatatga aaaaaagtac gcacgcggcg caagtcggtt
    25081 ttcgtgggtg agctgaatgt tttcccagcg attcataggg cctcccggta gagagaaatc
    25141 ggaaccattc atggtgatgg tagtttgtgt aattgctggg ggtttagtaa aatttttact
    25201 aaaagtagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    25261 ccggcaaaaa tgattgccgg acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    25321 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    25381 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgtacg gtggagagcg
    25441 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    25501 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    25561 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    25621 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    25681 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    25741 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    25801 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    25861 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    25921 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgt
    25981 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    26041 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    26101 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    26161 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    26221 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    26281 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    26341 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    26401 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    26461 gctatagttt agtaaaattt tacccgaagg gaggaagcaa tggaaaccgt ggcttacgct
    26521 gattttgcac gtctggaaat gcgcgtcgga aagattgtgg aagtgaaacg ccatgaaaac
    26581 gccgacaagc tgtacatcgt acaggttgat gtggggaaaa aacactgcaa accgtgacca
    26641 gcttggtgcc gtactacagc gaagaggagc tgatggggaa aacggtggtg gtcttatgca
    26701 atctgcaaaa agcgaaaatg cgtggtgaaa cgtcggaatg catgctgttg tgtgcggaaa
    26761 ccgatgacgg cagtgaaagc gtgttgttaa cgccggagcg gatgatgcct gcgggcgtac
    26821 gcgtcgtata aaaagatcgg atggcgacgt cgtatcgcca tccgatttga tattacgctt
    26881 cttcgacact tactcgcatg gccgccagcg ccttacgcgc cgctttgatc accagttcac
    26941 actgttcaat ggtcagtgtc agtggcggtt caatgcggat cgttttggcg ttattgagcg
    27001 ttccggccac cagtacgcgc tggcggaaca tctcgctggc aaagttatag ccgatttcgt
    27061 tatcaacaaa ctcaatcgcc atcaacatcc ctttaccacg cgcttcctgt accagatcgg
    27121 gatattcccg cgccagttga cggaaaccgt ccagcaacac attgatggtc gccagcgccg
    27181 ccgcacaggc cagcgggttg ccgccaaagg tggtggtatg caggaatggg ttgtcgaaca
    27241 gaactgaaaa cacctcttca gtggcgatgg tcgcgccaat cggcatcacg ccgccgccga
    27301 gggcttttgc cagacacaga atgtccggct gaacgttctc atgttcgcag gcaaacatct
    27361 tgcccgtgcg ccccatgccc gtttgtactt catcgaggat catcagtgcg ccgaactcat
    27421 cgcacagctt acgcacggcg gtgagatagc ccggcggcgg cagaattacg ccaccttcgc
    27481 cctgaatcgg ttcgaggatc accgcagcca catcatcacc ggtttttttg cactcattaa
    27541 gagccgtgcg catggcttcg atattgccaa acggcacatg acggaaccct ggcagcagtg
    27601 gcataaacgg tttgcggaag gtcgatttcg ccgtggccga cagcgcgcca agtgatttac
    27661 cgtggaacgc gccgctggtg gcaataaaag taaatttgcc gcgcggcgac tgataagcct
    27721 tcgccagctt cagcgccgct tcaacggact cggtgccgct attacagaag aagctgtatt
    27781 tcagtttacc gggcgttagc gcagcaaggg ttttcgccag catagcccgt agcggatcga
    27841 gtagctcctg gctgtgcagt ggttgtttcg caagttgatt ctgtacggcg gaaaccacaa
    27901 ctggattacg gtgccccacg ttgaaaattc caaaacctcc caggcagtcg ataaactcct
    27961 gtccctgggt gtcgacaagc gtatttaaac ctcccgcttg ccactctacg gctccgtaat
    28021 ccccgccggc ggtaacagat ttgcgatact ctaaaaaccc cggattgaca tgctctttga
    28081 agtattcaat cacctctcgg ttaagtgctt tcatctcctc atgatccagc gttcgcttct
    28141 caatgagatt cagggcgtgg gcgctgcacg ctaaagccga tgcgctcgaa ggtaacctgt
    28201 tcaaaatatg ctccggaggc tcgcgtatca catgataccg atttaagtat tgcagggatt
    28261 gcgccactcc ggctgcgatc gcgaaaatcg ggataaacac aaatgcggtt atcatttgtg
    28321 caatatttaa tgctatccgt taacatttgt tgttattttg cacggttttg tcgggtatgg
    28381 atttgatgat tgctgcgcaa atgcacgttt tcagggcaat aattgcctga tttttgggca
    28441 agataattaa cgttttcgta tagtaacaga gtgaaagtat tttaaatcaa agaattagat
    28501 ttgtgcttta atcgcaaatt gcgatctaaa tcaaattaat cggttaaaga taaccgcagc
    28561 ggggccgaca taaactctga caagaagtta acaaccatat aacctgcaca ggacgcgaac
    28621 atgtcttctc atccgtatgt cacccagcaa aataccccgc tggcggacga taccactctg
    28681 atgttcacta ccgatctgca aagctatatc actcatgcta atgacacttt tgtgcaggtg
    28741 agcggcttta ccttgcaaga gttacaaggg cagccgcaca acatggtgcg tcacccggat
    28801 atgccaaaag cggcgtttgc ggatatgtgg ttcaccctga aaaaagggga gccctggagc
    28861 ggcatcgtga aaaatcgccg caaaaatggc gaccattatt gggtgcgggc caatgcggta
    28921 ccgatggtgc gcgagggaaa aatcagtggc tatatgtcga ttcgtacccg ggcgacggat
    28981 gaagagattg cggcggtgga gccgctgtac aaagcgttga actccggacg taccagtaag
    29041 cgtattcata aaggcctggt ggtgcgtaaa ggctggctgg gtaaactgcc ttcattaccg
    29101 cttcgctggc ggacgcgtgg agtgatgacc ctgatgttta tcttgctggc ggccatgctt
    29161 tggtttgttg ctgccccggt ggtgacgtat ttcctctgtg tgttagtggt attgttggca
    29221 agcgcttgtt ttgaatggca gattgtgcgc ccgatagaaa atgtcgcccg tcaggcactg
    29281 aaggtggcga ccggagagcg taatagtgtt gagcacctga atcgcagcga tgagctgggg
    29341 ctgacattac gcgcggtagg gcagcttggc ctgatgtgcc gttggctaat taacgatgtc
    29401 tcaagccagg tgtccagtgt cagaaatggc agtgagacgc tggcgaaagg caccgatgaa
    29461 ctgaacgaac atacccagca gacagttgat aacgttcagc aaacggtggc gaccatgaac
    29521 caaatggcgg cgtcggtgaa acagaactct gccacggcgt cggctgccga taaactttct
    29581 atcaccgcca gtaatgcggc agtgcagggt ggggaggcga tgaccacggt gatcaagaca
    29641 atggacgata tcgccgacag tacccagcgc attggcacca ttacttcgct gattaacgat
    29701 attgcgtttc agaccaatat tctggccctg aatgcggcgg tggaagcggc gcgtgccggc
    29761 gaacagggca aaggttttgc agtggtggca ggggaagtgc gtcatttagc cagccgcagc
    29821 gctaatgctg ccaacgatat tcgcaagctg attgatgcca gtgctgataa ggtgcaatcc
    29881 ggttcgcagc aggtacacgc cgccggacgg acgatggaag atattgtggc acaggtgaaa
    29941 aacgtcaccc agttgatcgc ccagattagc cattcaacgc tggaacaggc cgatgggctt
    30001 tccagcctga cccgtgcagt ggatgagctt aacctgatca cccagaaaaa tgccgagctg
    30061 gtggaagaga gtgcgcaggt gtcggcgatg gtgaaacacc gcgccagccg actggaagac
    30121 gcggtgacgg tgctgcatta atcggtatgc cggatccggc ggttggagca caatacctga
    30181 tgcgatgctg gcgcatctta tcaggcctgc ggggtgtgta gcgggtgtag gccggataag
    30241 gcgcgtcaag cgtcgcatcc ggcaattgca ccgcgccact ggcggatgcg gcgattaatc
    30301 cagttgcgta atatcaaaag cggcgcggtc gatcaccgca atgatctttt ttatttgtgc
    30361 atcactgata tcgctctggt tgacgcgtaa atccagcacc gctttaaaat tatccagcgc
    30421 ccgcttcatt tgcgggttct ggcgcagcgc cgcgccaacg caacgcgctt tgatgcgttc
    30481 ttcaatcatc tccacctgtt cgcggttttc ttccagccac tgcgcgcctt gttcggtcag
    30541 cgcaatctgc ttcttaccac cttcctcttc gcggatggta atcagcgact gctcctgcag
    30601 aaaatccagc gtcgggtaga tgacgcccgg gcttggggtg taattcccct gggttagatt
    30661 ctcaatcgct ttaatcaatt cgtaaccgtg gctgtcatcg cgcgagagaa tatccagaat
    30721 caccagacgc aattcaccgt gaccaaagaa acgttgccga cgaccgccgc cgcgaccgtg
    30781 gcgaccaccg caacattgac cgtgttcatg ctggtgaccg tgtccgcagt gctcgtgttc
    30841 tgacttctca cctttgcagc agccctcatg gcgtggctgg ccttcatgtt tacaacaccc
    30901 ttcgtgatga tggctcatat cagcctcctt ttgcttgatt agatatatct aatttatatc
    30961 tgatgtgagc attactgcaa gtacaaaaat gatttttaaa cgtggaagca cctcacatct
    31021 aggccggata agacgttcac tccgcatccg gcagcattgc tatggcactg aatgccttca
    31081 catacttcag acatatcagt tctacaaatc gcttgcattt atcatgatta acaatcatta
    31141 tcatttgcga attttattta gatatatctg attaccatca cgaaggcgat aacaatgaat
    31201 aacacccccc gctacccgca gcgcgttcgc aatgatctgc gcttccgtga actgactgtg
    31261 ctacgcgttg agcgcatcag cgccggtttt cagcgcattg tccttggcgg cgaggcgttg
    31321 gacggtttta catcgcgcgg ctttgacgat cacagcaaac tcttctttcc tcaacctgac
    31381 gctcactttg tgccgccaac ggtaacggaa gagggcatcg tctggccgga aggaccacgc
    31441 ccaccgtcgc gtgactatac gccactgtat gacgaactac gccatgaact ggcgatcgat
    31501 ttctttattc acgacggtgg ggtcgccagc ggctgggcga taaacttacg gtggcaggtc
    31561 cgcgcggttc gctggtggtg ccggaagatt acgcgtatca gctgtatgtc tgcgatgaat
    31621 ccggaatgcc cgcactgcgc cgccgcctgg aaacgttgag caaacttgcc gttaaaccgc
    31681 aagttagtgc gctggttagc gtgcgggata acgcctgtca ggattatctc gcgcaccttg
    31741 atggttttaa tatcgaatgg ctggcacatg atgagcaggc ggtagatgcg cgtctggcgc
    31801 agatgcaaat ccctgccaga tgattacttc atctggataa ccggcgaagg taaagtcgtt
    31861 aagaatttaa gccgccgctt tgaagcggaa cagtatgacc cacagcgggt acgtgcagcg
    31921 gcttactggc acgcaaagta actgatatat aaaaagaaaa aggctgacga tttctcgtca
    31981 gcctttgcta tatctggtgg cccctgctgg acttgaacca gcgaccaagc gattatgagt
    32041 cgcctgctct aaccactgag ctaaggggcc atggtagcgg attataaagt aactccgtgt
    32101 cgcaatccag ccattaccgc gcgcctgctg tttttataaa caatgtattt tcaatccgtt
    32161 atacttttcc tggtgatgtt agaaaggagt aaatatggtt gaggatattt tggctccagg
    32221 gttacgggtc gtgttttgcg gtatcaaccc tgggctttca tccgccggga ctggttttcc
    32281 ctttgctcat ccggcaaatc gcttctggaa ggtgatatat caggccgggt ttaccgaccg
    32341 tcagttgaag ccgcaggagg cacagcatct gctggattat cgttgtggcg tcaccaaact
    32401 ggtagaccgt ccaacggtgc aagccaatga agtttcaaag caggagctgc acgcaggtgg
    32461 gcgtaagctg attgaaaaaa ttgaagatta tcagccgcag gcgttggcga ttctgggcaa
    32521 acaagcatat gaacagggat tcagccagcg cggtgcacag tgggggaaac aaacgctcac
    32581 cattggttcg acgcagattt gggtgctgcc aaatcccagc ggtttaagtc gcgtttcact
    32641 ggagaaactg gttgaagcgt atcgcgagct ggaccaggcg ctggtagtgc gtgggcgata
    32701 aaaaacgcca ccga
//
LOCUS       DAJTYG010000040        32329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTYG010000040 DAJTYG010000000
VERSION     DAJTYG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.040"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO568_001973"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO568_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6641334.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OO568_001974"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OO568_001974"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6641335.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OO568_001975"
     CDS             complement(3736..4092)
                     /locus_tag="OO568_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6641336.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OO568_001976"
     CDS             complement(4096..4512)
                     /locus_tag="OO568_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6641337.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRSVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO568_001977"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO568_001977"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6641338.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5960
                     /locus_tag="OO568_001978"
                     /pseudo
     CDS             5738..5960
                     /locus_tag="OO568_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="OO568_001979"
     CDS             complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="OO568_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6641339.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7910..8989)
                     /gene="alr"
                     /locus_tag="OO568_001980"
     CDS             complement(7910..8989)
                     /gene="alr"
                     /locus_tag="OO568_001980"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6641340.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9042..10457)
                     /gene="dnaB"
                     /locus_tag="OO568_001981"
     CDS             complement(9042..10457)
                     /gene="dnaB"
                     /locus_tag="OO568_001981"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6641341.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10540..11523
                     /locus_tag="OO568_001982"
     CDS             10540..11523
                     /locus_tag="OO568_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6641342.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="OO568_001983"
     CDS             complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="OO568_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6641343.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="OO568_001984"
     CDS             complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="OO568_001984"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6641344.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13465..14450)
                     /locus_tag="OO568_001985"
                     /pseudo
     CDS             complement(13465..14450)
                     /locus_tag="OO568_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14768..15283
                     /gene="zur"
                     /locus_tag="OO568_001986"
     CDS             14768..15283
                     /gene="zur"
                     /locus_tag="OO568_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6641345.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15325..15534)
                     /locus_tag="OO568_001987"
     CDS             complement(15325..15534)
                     /locus_tag="OO568_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6641346.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15650..16975)
                     /gene="dinF"
                     /locus_tag="OO568_001988"
     CDS             complement(15650..16975)
                     /gene="dinF"
                     /locus_tag="OO568_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6641347.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17048..17656)
                     /gene="lexA"
                     /locus_tag="OO568_001989"
     CDS             complement(17048..17656)
                     /gene="lexA"
                     /locus_tag="OO568_001989"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6641348.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17766..18134)
                     /gene="dgkA"
                     /locus_tag="OO568_001990"
     CDS             complement(17766..18134)
                     /gene="dgkA"
                     /locus_tag="OO568_001990"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6641349.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18305..20728
                     /gene="plsB"
                     /locus_tag="OO568_001991"
     CDS             18305..20728
                     /gene="plsB"
                     /locus_tag="OO568_001991"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6641350.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20883..21755)
                     /gene="ubiA"
                     /locus_tag="OO568_001992"
     CDS             complement(20883..21755)
                     /gene="ubiA"
                     /locus_tag="OO568_001992"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6641351.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21768..22265)
                     /gene="ubiC"
                     /locus_tag="OO568_001993"
     CDS             complement(21768..22265)
                     /gene="ubiC"
                     /locus_tag="OO568_001993"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6641352.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLFDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22488..24068)
                     /locus_tag="OO568_001994"
                     /pseudo
     CDS             complement(22488..24068)
                     /locus_tag="OO568_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24297..25217)
                     /gene="malM"
                     /locus_tag="OO568_001995"
     CDS             complement(24297..25217)
                     /gene="malM"
                     /locus_tag="OO568_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6641353.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKIQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLIIDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTMEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25369..26709)
                     /gene="lamB"
                     /locus_tag="OO568_001996"
     CDS             complement(25369..26709)
                     /gene="lamB"
                     /locus_tag="OO568_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6641354.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26781..27896)
                     /gene="malK"
                     /locus_tag="OO568_001997"
     CDS             complement(26781..27896)
                     /gene="malK"
                     /locus_tag="OO568_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6641355.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28261..29451
                     /gene="malE"
                     /locus_tag="OO568_001998"
     CDS             28261..29451
                     /gene="malE"
                     /locus_tag="OO568_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6641356.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29605..31149
                     /gene="malF"
                     /locus_tag="OO568_001999"
                     /pseudo
     CDS             29605..31149
                     /gene="malF"
                     /locus_tag="OO568_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
     gene            31164..32054
                     /gene="malG"
                     /locus_tag="OO568_002000"
     CDS             31164..32054
                     /gene="malG"
                     /locus_tag="OO568_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6641357.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8229 a         8289 c         7802 g         8009 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtgaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acgctgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctcttatg ccaaagattt ctttttatac atagaaacac agttaaaaat
     5881 tgcaaaagat ttttgtagac ctggagaaga agtatcaagt tctattgcaa gtaaagtttt
     5941 tcacgcattt attgatttag ttaacaaaat caggggtaag aaagacttta tgtatatttg
     6001 cacgctttgc tgttttgccg aggaggttaa aggtgattat tctcattaca ggaccttttt
     6061 atttgatatt ggtaatcaat ataaggttaa acttatacaa agcggaaaaa aagagttctc
     6121 tttaacatta gaatttaacg atactataat tgaatctcag aaagtcacag gcaataaggc
     6181 aaagcatatt cttgaggata tagaaatatt ctatcgtaac aaacccgata cttattatta
     6241 aatttaataa tggttagatg tgcagaaatt attactccac acgaaaaaat agggaaagga
     6301 attccagata attgggataa acttaacaat aaaagaactg acaacgtttt attatctctc
     6361 acgtagaaca acggcatcaa aaaaatcatc tacaaagaaa tagtgaaagc gacgacagtt
     6421 taatttcact gcaggctggg taactccagc ctgctttcct gcattacatc accgcagcaa
     6481 acgcctttgc cacacgttgt acatttgccg tatttaaccc ggcgacacac atgcgaccgc
     6541 tggcgatgag atagacacca aattcttcac gtagtcggtc aacctgagcg gcacttaaac
     6601 cggtataact gaacatgccg cgctgattaa gcagataatc gaaattgcgt tctggcatct
     6661 ctgtgcttaa taccttcacc aattcctgac gcattgccag aatgcgagta cgcatctctt
     6721 ctacttccgc cagccagctg gctttcaatg cctcgtcatt cagcactgca gccaccacct
     6781 gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac tgttgctttc aattgcccca
     6841 gtacgcggcc agcggcttcg gcatcttcac acagaacaga aagtccgccg acgcgctcgc
     6901 cgtaaaggga gaaaattttc gagaacgaat tgctcaccag agcgggtaat ccagcgctgg
     6961 caatggcgcg aatagcgtag gcatcctctt ccataccggc actaaatcct tgataggcaa
     7021 tatcgaggaa tgggataagc tcgcgggctt tgagaatttc aatcaccgca tcccactgat
     7081 cattagtgag atcggcaccc gttgggttgt ggcaacatgg atgcagcaac acaatactgc
     7141 gggcaggtaa tgttttcagc gtcgccaaca ggtcattaaa gcgcacgctg ttagtcactt
     7201 cgtcatacca ggggtaagta ctcacttcga atccagcccc ggcgaatatt gctacgtggt
     7261 tttcccaagt aggatcgctg acccagacgc ctgattccgg gaagtagcgt ttcaggaaat
     7321 ccgcgcccac tttcaatgcc cctgagccgc caagggtttg aatggttgct acgcgctgtt
     7381 gttgcagtac cggatggtcc gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt
     7441 taagcccttc catcggtaaa taaagcgaag cgccatgggg ctgcgcattc aggcgcgctt
     7501 ccgcctccgc cacggctttc aattgtggaa taattccgtc ttcgttgtag tacagaccga
     7561 tacttaaatt cactttgtcg ctgcgagggt cttctttaaa acgctccata agcgtaagaa
     7621 tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg atggttctcc aggtttacgg
     7681 gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga tcgagtggat gttcaggagc
     7741 gaacggcaat tagcaacaga gtgagactaa tgataaacgt acatccgcca gaggcacgac
     7801 ctttataaga gcatgaaaga acatcaactt attgaatttt taggatttta ttggccggat
     7861 aaggcattca cgccgcatcc ggcacagaca atcaaatatt acagaacgat taatccacgt
     7921 atttcatcgc gacccttgaa gtcaggcgcg taataagttc gtaagcgctt actttcgtca
     7981 tttcagcgat acgttctacg ggcaaacctt cgccccataa aatgaccgga tccccggctt
     8041 tgtcctgcgc ctgtggacct aagtctacgc agatcatatc catcgccacg cgcccgacaa
     8101 tcggtacttc gcgaccgttc accagcactg gcgtaccgga cggcgcggcg cgcggataac
     8161 catcgccata gcccatcgcg actacgccaa gacgggtatc acgttcgctt acccaggttc
     8221 caccataacc aacaggctct ccggctttat gctcacgcac ggcaatcagg ctggaggtta
     8281 gtgacatcac tggctgacag ccaaaatcgg caccggtgga gcgatcttcc agcggcgaga
     8341 cgccataaag aatgatgccc gggcgcaccc agtcaaaatg cgactgtggc cacagcagaa
     8401 tgccacccga cgcggcaatg gaacgttgac caggtttgcc ttcgcaaaag gtattaaaga
     8461 tagcgagttg tttctcggtt gcgccacatt ttggttcatc cgcgcgcgca aaatggctga
     8521 cgatattcac cggctgacga acgtttttgc actgggtcag gcgatgataa aacgcctcag
     8581 cctgttccgg ccttacgccc agacggtgca taccggtatc gagtttcatc cagacggtaa
     8641 ccggctcgtc caagctagcc tcttccagcg cagccagctg ttcttcgtta tgcacggcgg
     8701 tatgaaaatg ttgcgcagaa atcgtcggca gatctctggc atcaaaaaag ccttcgagta
     8761 acagtacagg tttggtgatt ccccccgcac gcagtcgcag agcttcttcg agacgggcta
     8821 cgccaaaggc gtcagcatcg gggagcgttc gcgcggtctc aagaagaccg tgaccataag
     8881 cgttcgcttt caccaccgca accattttac tggcaggtgc cagttcacga agacgttgca
     8941 ggttgtgtcg cagagcgcgg cggttaatca caacagttgc cgcttgcatt tgtgttcctt
     9001 gataattgtt tgctttaatt acctaattca taaaataatt attattcgtc gtcgtactgc
     9061 ggccccgcat agttgtcgaa gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg
     9121 attgggccgt tacgttgttt accgataata atttccgcga tgccttttaa atcactgttt
     9181 tcgtgataca cctcatcacg atagataaac atgatcaagt ccgcatcctg ctcgatagag
     9241 ccagattcac gcaggtcgga gttgaccgga cgtttgtcgg cacgttgttc cagagaacgg
     9301 ttcaactggg acagcgccac caccggcacg ttcagttctt tcgccagtgc tttcagcgag
     9361 cgggagattt ctgcaatttc cagcgtacgg ttatcggaaa gcgccggtac gcgcatcagt
     9421 tgcaggtagt cgatcatgat aagcccgatg ccgccgtgtt cacgggcaat acggcgtgcg
     9481 cgggaacgca cttccgttgg cgtcaggccg gaagagtcat cgatatagat attgcgtttt
     9541 tcgagcaaaa tacccatggt gccggaaatg cgcgcccagt cttcatcatc gagctgcccg
     9601 gtacggattt tagtctggtc aacgcgcgac agcgacgcca gagaacgcat catgatctgt
     9661 tctgatggca tctccagcga gaagataagt actggtttat cctgcaacat tgccgcgttt
     9721 tcgacgaggt tcatcgcaaa tgttgtttta cccatcgacg gacgcgcggc gacgatgatc
     9781 aaatccgacg gctgcaagcc agcggttttt ttgttgagat cgtcataacc ggtgtttacc
     9841 ccggtaacgc catcgtgtgg ctgctgaaac aactgctcaa tacgcgccac ggttgcgtcg
     9901 agcacatcgg cgatgttctt cggcccttcg tctttgtttg cacgactttc ggcaatttta
     9961 aagacgcggg attcagcaag gtccagcaga tcttcgctgg tacgcccctg cggatcaaaa
    10021 ccggcttcag caatctcatt cgcaaccgag atcatctcac ggacaacggc acgttcacgc
    10081 acgatgtccg cataagcact gatgttcgcc gcacttggcg tattttttga cagctctgcc
    10141 agataagcaa aaccaccgac gctatcgagt tgcccctggc gttccaacga ttccgcaagg
    10201 gtaatcagat cgataggact accgctttcc tgcaaacgcg ccatttcagt aaagatatga
    10261 cggtgtgggc gggtgtaaaa atcgtctgct accacacgct cggctacatc atcccagcgt
    10321 tcgttatcca gcattaaacc gcccaacacc gactgctccg cttcgatcga gtgcggaggc
    10381 actttcagcc cggcaacttg tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag
    10441 ggtttatttc ctgccatagt gaatggagtt accgaaataa agaatgggtc gaaactttac
    10501 catatgaagc agactctgac gatacgttct ggaggacaca tggcaacacg aattgaattt
    10561 cacaagcacg gtggcccgga agtacttcaa gccgtagagt tcactcctgc cgatccggcg
    10621 gagaatgaaa tccaggtcga aaataaagcc atcggcatca attttatcga cacgtatatc
    10681 cgcagcggcc tttacccgcc gccatcgcta cccagcggat taggtaccga agcggcaggc
    10741 atcgtgagta aagtcggcag tggtgtaaag catattaagg ctggcgatca tgtagtctat
    10801 gcgcagtcgg cgttaggcgc ttacagctct gtgcataaca ttaatgcgga taaagcggcg
    10861 attctgcctg cggcaatttc ttttgagcaa gctgcggcat ccttcctgaa aggcttaacg
    10921 gtttattatc tgctgcgcaa aacctatgaa attaaacccg atgaacagtt cctgttccac
    10981 gcagcggctg gcggcgttgg cttaattgcc tgccagtggg caaaagccct gggcgcgaaa
    11041 ctcatcggca ccgtaggaac cgcgcaaaaa gcgcagagcg cgctaaaagc cggcgcgtgg
    11101 caggttatta actatcgtga agagaatctg gtcgagcgat tgaaagagat caccggcggt
    11161 aagaaagtgc gcgtagtgta cgattccgtg ggcagagaca cctgggaacg gtcgctggat
    11221 tgcctgcaac gccgcggctt aatggtcagt tttggcaact catcaggtgc ggttaccggt
    11281 gtgaacttag gcattctcaa tcaaaaaggc tcgttgtatg tgacacgccc ttccctgcaa
    11341 ggctatatca ccacgcggga ggaattaacc gaggccagta atgaactgtt ctctttgatt
    11401 gccagcggtg tgattaaggt cgatgtcgcc gagcagcaga aatatccgct gaaggatgcg
    11461 cagcgtgcgc atgagattct ggaaagccgg gcgacgcaag gttccagcct gctgattcca
    11521 taaaagaaat agggcttcca cctgggaagc cctttctttt tatagttcgg ctgtatgtag
    11581 ggtacagcac gatgaatctg ttagaggcgc aataatgaca gatttgatta tcaattccta
    11641 ttttgttcta aggataaaac cttaggttgt gatcatccgc acaatccctt agtaacgcca
    11701 gcggtcataa cgctgatatt tcggcatttt tggtgcttta atcgccttaa taacccacac
    11761 caccgcaatc gccagtagta accacggcag caacttaatc atcaatgcca gcataccgcc
    11821 gaggaacata atggccgtcg ccacaaccag cgcggcgata atgcccagca acgaaacgcc
    11881 ggtgaccatc agcatgacaa aaaagccaat cacaaaaagt agttccagca tgatgctctc
    11941 ccaaatatga aatctcttgc tggcattaca agaatcatgc caaaaataat ctattgattt
    12001 aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga atttgactac tttttggtga
    12061 aaagttaacg cttatccgcc accagtttga gcgcgtgttc cagcacgtta atgtcagcac
    12121 ccgctttatg ggcattttca cttaaataac gccgccactg ccgcgcgcca ggaataccct
    12181 ggaacaaacc cagcatatgc cggataatat ggccgagata cgtcccctgg ctgagttcat
    12241 gctcaatgta cggatacatg gcacgcacta ctgccaccgg atcggcatcg gtatccgagg
    12301 aaccaaagat ctcccggtct accgccgcca gaatacccgg attctgatac gcctcgcgcc
    12361 cgaccatcac gccatccata tgttgcaaat gcgctttagc ttcttccagc gacttgatac
    12421 caccgttaat cgacattgtc agatgcggga agtcacgctt cagttgatac acacgcggat
    12481 aatcgagcgg cgggatttca cggttttctt tcgggcttaa ccccgaaagc caggctttac
    12541 gtgcgtggat gatgaacatc tcacactcgc ctttgccgga aacggtattg atgaaatcgc
    12601 agagaaattc atagctgtcc tggtcgtcga tgccaatacg cgttttcacc gtcaccggaa
    12661 tcgacaccac atcgcgcatc gctttcacgc agtcggcaac cagctgcgca ttacccatca
    12721 gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg gcagccgaca ttcaggttga
    12781 tctcatcata tccgcgcgct tctgccagct ttgcacactg cgccagcgcc gccggatcgc
    12841 taccgcccag ttgcaacgct accggatgtt cttcttcact gtacgccaga taatcacctt
    12901 taccgtgaat aatcgcccct gtggtcacca tttcggtata aagcaacgta ttgcgggaaa
    12961 gcagacgcaa gaaatagcgg caatgtctgt ccgtccagtc gagcattggt gcaacactaa
    13021 accgaccact ccagtgaacg tcagtttttt caggcattgc gctggtttgg ttgatttttt
    13081 gcatttcaga attaccgtgc attttcaaat gtagagatta ttttatcgat atatcatggg
    13141 gttatgttat tcagcttcac tgttcaggag gctcaatagc ggggtactat accataacaa
    13201 caggaagcgc ctgtctcatt gcaaaagaaa attgagatcc tctcaaggca tgaagctctc
    13261 acgaagtgat ggaaataatc ttattagccg ttaactttgt taaggccaat gataaacaat
    13321 ccaggttcga cgataaataa aaaatcacac attaaactct ggtgatatat ctccctgcta
    13381 atagcattga tagagaaaaa agaacccaat aaatattggg tccttttata taatgccttc
    13441 catactatcg aagaacttca catattattt ctccgattta accccgaaca agtcataaat
    13501 taatttagaa gtgtctgtaa gtattttaat ctcttccttt gaggttgggt caaacgactt
    13561 cgcgaagcta attaattgtg gtgcagcatc tcgcattttg cttaaaatat caggatcgag
    13621 cgttccttca ttcaccagat gtgacatctt atccagatag ccatcaaaat tcatttctcc
    13681 cccatctgcc agacgcttca tctctcccag gtacttcttc atatcacgtt gatataattt
    13741 ttcaaactga gctttcaggt agctctcatt attttcatta atatacactg tttctgataa
    13801 atctgcgtag gcactagaat atgaaagtga aattaacagc gaagcaaata attttaagct
    13861 gttgtcatca tgttcctggc aggcattaac aaacgttaaa aaaccagaac ctattgcttg
    13921 aaaatgtata ttcgctaaag gttcattatc cttagattcc tcataaaata gctgaaccgt
    13981 ggagggaagg ccattcttag tttctacatc aaaagtacat tgttcaatgg gcattgcatc
    14041 tttattatcc ccaggattac atatactatt aatcgcgaaa tgaaaaatag cccggtcaac
    14101 aactgatgca ataaagtcac gatcgtttaa atcctcatca gtgcaatcgt cagtatactt
    14161 attgacccac attttgagat catttatttg ctcaattaaa aacacatttt tttacgtgca
    14221 tgccataaat tatgcgtgat tcgttgaata aataaatcta tttttgcaat aattatatcc
    14281 cgatgacgca gcgctgcata atcaatagat ttaccatata tattatttaa tttacctttg
    14341 attgaacctc tattatccaa tttatctttg gttgcatctt tattttcgaa ttgatttgaa
    14401 ttgagtgttt tattaaagta tccagttgca ccgggtatta tcttcaacat gattcatcta
    14461 ccttttttgt cgtaccgtgt cctccttata attctttttc catctggtca ctatcaaaaa
    14521 tcgctcataa tttctgaact acaggtatgc gtttaaaccc agttaaataa atagcgagtt
    14581 aacttgaagc aatttattta aaaatgaatt attaattatt atcgtgttga taatggtacg
    14641 acaaggcaac acaacacctt acctacaaaa cagaaaatca acattacggc aacaataagg
    14701 gttctcggtg tttttcctgg aacatggtaa agtaaggaca ttcttaaccc ccactttgag
    14761 gtgcccgatg gaaaagacca caacgcagga gttattagcg caggctgaaa aaatctgcgc
    14821 gcagcgtaat gtgcgcctga ccccacagcg cctggaagtg ttgcgcctga tgagtctgca
    14881 ggatggcgct atcagcgctt atgatctgct tgatttactg cgcgaagctg aaccgcaagc
    14941 caagccgcca acggtttatc gcgcgctgga ttttctgctt gagcaaggtt ttgtgcataa
    15001 ggtggaatcc accaacagtt atgtgctctg tcatctgttc gaccagccca cgcatacgtc
    15061 agccatgttt atttgcgatc gctgcggcgc ggtgaaagaa gagtgtgcag aaggcgtgga
    15121 agacattatg catacgctgg cggcaaaaat ggggtttgcc ctgcggcata atgtgattga
    15181 agcacatggg ctctgtgcgg catgcgtgga agtggaagcg tgtcgtcatc ctgaacagtg
    15241 ccagcatgat cactctgtgc aggtgaaaaa gaaaccgcgt taagagggcg tacatccttg
    15301 tacacgtcgg gcaggaggga ttaattacca gcgatattca ttgcgggttt cccaatccac
    15361 gacctctttt tctgcctgat ctttctgata accataacgt tcctggattt taccgaccag
    15421 ttgatcacgt ttaccttcaa tgatcgtcat atcatcatcg gtcagtttgc cccattgctc
    15481 tttcacttta cctttaaact gtttccagtt accgccggct tcatctttat tcataatcaa
    15541 gacctcatcg ttaggttgtg aatgagagta cgttcacttt tcttctgaac gtgagattaa
    15601 gtgtagtcag caatttggct taggataatt tattcagaat ttttaaccgt cacgttgcgg
    15661 caaaccaggt accgttgcgc cagtgacgcc gccagatagc cgccagagaa agcccgcgca
    15721 acgccagaaa gacggttaat gccagccaca aaccatgatt acccagccac ggcagcgtaa
    15781 ggagcgtcag cgcaaaacct gcggcggcca ccgccatact gttacgcatt tcggcggcac
    15841 gcgttgcgcc tataaacatg ccgtccagca gataacacca gacgccaacc aacggcaaaa
    15901 tcacctgcca gataagatag cggtcagcca gctgctgaat ctgggttaac gacgtcagca
    15961 acgcaatgat gtgttcccct gccagcaaat aaaccaccga aaacagtaac gctacaatcc
    16021 ccgactggcg gcacgctgcc cgccatacat ccagcaactg gctaccgtcg cgcgcaccgt
    16081 atgcctgacc ggagtgcgct tcaaccgcgt aggcaaaacc atcaagcgca taggcggtaa
    16141 aggtgagtag cgtcatcaga accgcgttaa cagcgataat gtcactcccc agtcgcgcac
    16201 caagtacggt gatcgcgccg aaacagagtt gcaacaacag cgaacgcagc atgatatcgc
    16261 ggttaagcgc cagcaagcga cggaagtttc ctcgccaggc agttttcagc atttcgccgg
    16321 aaattccgcg tagtttgagg attttacgca ccattagcag accaatcagc aatgttgcat
    16381 attccgcaat aaccgtcgcc agcgccgcgc cctgcacgtt catatgcagc cccatcacca
    16441 gccagacatc cagcacaatg ttgaggatat tgccgaccac taacaaaatt actggcgcac
    16501 gggcatattg cacgccgagt aaccaaccga gtaataccag attcgccagc gacgccggag
    16561 cgcttaacca gcggatttca agaaagcgcc gcgcctgttc caggaccgct tcactaccgc
    16621 caacaatatg cagcgccaga tcgataatcg gcgtacgcaa cagcgcaatt aacgccccgg
    16681 cccccaacgc caacagcaac ggttgcacca gcgcacgggc taatgcctga ggatttttgg
    16741 caccataagc ctgcgcagtc agcccggtgg tgctcatgcg taaaaacagc aacagcataa
    16801 agagaaagct ggtcgccgtt gcaccaaccg ccacgccgcc caaataaacc ggactatcaa
    16861 gatgaccaat taccgccgta tcgaccagtc ccagcaacgg aacggtgata ttggagaaaa
    16921 tcatgggtaa ggcgagatgc cagagtgctt tatcagatga agtgaggaat gccatgcaga
    16981 caagcctgat gaagagagat gaaaaacaaa ccgcgacgcc aggcggcatc gcggtctcag
    17041 agatatgtta cagccagtcg ccgttgcgaa taaccccaac cgccagccct tcaatggtga
    17101 agctctgctg acgaaggtca acgacaattg gtttaaactc gctattttct ggcaacagtt
    17161 cgactttatt gccctgtttt ttcaggcgct taacggtaac ttcgtcatca atacgtgcga
    17221 caacgacctg accgttacgt acatcctgag ttttatgcac tgccagcaag tcgccatcca
    17281 taatgccgat atctttcatc gacatcccgc tgacgcgcag caggaaatca gcattcggct
    17341 tgaacaagga aggatcgacc tgataatgac cttcaatatg ctgttgcgcc agaagcggtt
    17401 caccggcagc cacacgacct accagcggca acccttcttc ctcttcctgc aacagacgaa
    17461 tcccgcgtga tgcgccggaa acaatttcaa taacgccttt gcgtgccagc gccttcagat
    17521 gttcttcagc cgcgtttggg gaacggaacc ccaaacgctg cgcgatttcc gcacgcgtcg
    17581 gcggcatacc tgtctggctg atgtgatcac ggatgagatc aaacacctct tgttgcctgg
    17641 ccgttaacgc tttcattccg ccccctgggt gtatatacag ttatactgtg agtatataca
    17701 gcaaaaggcg attttggaac cataaactgc acaataaacc agagatttat cgaaatctgg
    17761 aagggttatc caaaatgcga ccataacagg atgcaccagg taatcacggc gacgataatg
    17821 gcaatcagca ccgcggcgga tcccatatct tttgcgcgtc cggaaagctc atggtattca
    17881 gagccaattc ggtcaaccac tgcttcgatg gcgctattga ggatttccac aatcatcacc
    17941 agcatcacgg agctgataag cagcacgcgg gtaattgcgt ccacatccag ccagcaggcg
    18001 atgaccaccg ccaacaatac cgctacgcct tcctgacgga atgccgcttc gttgatccat
    18061 gcagcgcgta aacctttcca ggaatagcca gcagctttga taattcgggt gaatccagtg
    18121 gtattattgg ccattgaaag aaccttttta cattatgagc gtcaatatca gtgtaccgtt
    18181 aaatccgatg attcgggttt gcgtaacgcc gcgcgaaaca tgagcggata ccacagaatt
    18241 tcccatgact ttctgctatc cttgccgcgc atttgcatta ttaaccagag gctttacatc
    18301 gtttatgtcc ggctggccac gaatttacta caaattactg aatttaccat taagcatcct
    18361 ggtaaaaagc aagtctattc cggcagatcc tgccccggaa ctggggctgg atacctctcg
    18421 tccaattatg tacgttttac cgtacaactc gaaagcagat ttgctgacgt tgcgcgccca
    18481 gtgtctggca catgatttgc ctgacccgtt agagccgctg gaaatcgacg gcacgctact
    18541 gccgcgctat gtgttcattc acggcgggcc gcgtgtattc acctattaca cgccgaaaga
    18601 agagtctatt aagcttttcc acgactatct cgatttgcac cgtagcaacc caaatctgga
    18661 tgtgcagatg gtgccagtat cggtgatgtt tggtcgcgcg ccggggcgtg aaaaaggcga
    18721 agtgaacccg ccgctgcgta tgcttaacgg cgtacagaaa tttttcgctg tactgtggct
    18781 cggtcgcgac agttttgtgc gtttctcgcc gtcagtgtcg ctgcgccgta tggcggatga
    18841 acacggcacg gataaaacta tcgctcagaa actggcgcgc gtggcgcgta tgcactttgc
    18901 ccgtcaacgt ctggctgccg taggcccacg tctgcctgct cgtcaggatc tgtttaataa
    18961 gctgctcgcc tcccgcgcca ttgccaaagc ggtagaagat gaagcgcgca gcaaaaaaat
    19021 ctcccatgaa aaagcgcagc agaacgcgat tgcgctgatg gaagagattg cggcgaattt
    19081 ctcttacgag atgatccgcc tgactgaccg cattctgggc ttcacctgga accgatttta
    19141 tcagggcatc aacgtccata acgccgagcg cgttcgccag ctggcccacg acggtcatga
    19201 gctggtgtat gtgccttgcc accgcagtca catggactac ctgctacttt cttacgtgct
    19261 gtaccaccag gggctggtgc cgccgcatat cgccgccggg atcaacctga acttctggcc
    19321 ggccgggccg attttccgcc gtctgggggc gttctttatt cgccgtactt ttaaaggcaa
    19381 taaactttat tccaccgttt tccgtgagta tctcggcgaa ctgttcagcc gtggttattc
    19441 cgtcgagtac ttcgtggaag gcggtcgttc ccgcacgggg cgtttgctgg atccgaaaac
    19501 cggtacgctg tcgatgacca ttcaggcgat gctgcgtggc ggcacgcgtc cgattacgct
    19561 gattccgatc tatatcggtt atgagcacgt catggaagtg ggtacttacg ccaaagaact
    19621 gcgcggcgcg acgaaagaga aagagagcct gccgcagatg ctgcgcggtt taagcaagct
    19681 gcgtaatctc ggtcagggtt acgtcaactt cggtgaacca atgccgttga tgacctacct
    19741 taaccagcac gtaccagact ggcgtgaatc tatcgatccc atcgaagcgg tgcgtccggc
    19801 ctggttaacg ccgacggtca ataatattgc tgccgatctg atggtacgca ttaacaacgc
    19861 aggcgcggca aacgccatga acctgtgctg tactgcgcta ctggcatcac gtcagcgctc
    19921 actcacccgc gagcagttaa ccgagcaact caactgctac ctggatctga tgcgcaacgt
    19981 accttactcc acggactcta ccgttccttc agccagcgcc agcgagctta tcgatcacgc
    20041 gctgcaaatg aacaagtttg aagtcgagaa agacactatc ggcgacatca tcattctgcc
    20101 acgcgagcaa gcggtgctga tgacctacta tcgcaacaac attgcgcata tgttggtgct
    20161 gccttcgctg atggcggcaa ttgtcaccca gcatcgccac atctcccgcg acgtattgat
    20221 ggagcacgtc aatgtgcttt acccaatgct gaaagcggag ctgttcctgc gctgggatcg
    20281 cgacgagttg ccggacgtta ttgatgcgct ggcaaatgag atgcaacgtc aggggctgat
    20341 taccctgcaa gatgatgagt tgcatatcaa cccggcgcat tctcgcaccc tacagctgct
    20401 ggccgcaggc gcgcgcgaaa cgctgcaacg ttatgccatc accttctggt tgttgagtgc
    20461 caacccgtcg atcaaccgcg gtacgctgga gaaagagagc cgcaccgttg cgcaacgtct
    20521 ctccgtgctg cacggcatca acgcgccgga gttcttcgac aaggcggtgt tcagttctct
    20581 ggtgctgacg ctgcgtgatg aagggtatat cagcgatagc ggcgatgccg aaccagcaga
    20641 aacgatgaag gtttatcagt tgctggcgga gttgattaca tcagacgtgc gtttgacgat
    20701 tgagagtgcg acgcagggcg aagggtaatc agagagaatt gccggatgcg gcgaaaacgc
    20761 cttatccggc ctaccatgac ctgcaaattc aataaattgc gattcaccgg gtaggcctga
    20821 gaagcgcagc gcatcaggca acccagaaga aagccggatg atcatccggc ttttttacat
    20881 catcagaaat gccagtaact cattgccagt cctaaaaaca gtaccagacc aacatagtta
    20941 ttattcataa atgctttaaa gcaggcttca cgctcgcggt tggcaatcag tttttgttga
    21001 taaacaaaca gcgcgccagc caccacaatt gaccagtaat atccccagcc taagccattt
    21061 aactcaccga tgatcgccat cagtgccagt acgccaatct gcaaaatacc aataatcaat
    21121 ttatcgtatt ggccgaacag gattgccgtg gatttaatgc caatcttcac atcatcatcg
    21181 cggtcaacca tcgcatactg cgtgtcgtaa gccaccgccc agagaatatt ggcgaggaac
    21241 attaaccagc aactcaatgg caccgactca ctcacagcgg caaaagccat tggaatcgac
    21301 cagccaaacg ccgcgcccag caccacttgc gggagatggg tataccgctt cataaacggg
    21361 tacacccacg ccaatgccag agcggcaatc gacaacagaa tggtcatcgt attcagcgtc
    21421 agaaccagta aaaacgaaat cagtaccagc actacaaaca gcgcgcgcgc ctctttctct
    21481 gttaccgcgc cgctgggaag tggtcggttc gccgtgcgct taacatgacc atcaaacttg
    21541 cgatcagcat aatcattcac cacgcaacca gcagcgcgca tcagccagac acccgcgaca
    21601 aacaccgcca ggatccagag ctgaggaacg cccggtgtcg ccacccacaa cgcccataat
    21661 gttggccaga gcagcagtaa cgcgccaatt ggcttatccg tacgcattaa gcgatgaaac
    21721 gccagcagct tattctgcgt cagactccac tccatatttt tttcctctta gtacaacggt
    21781 gacgccggta aaaacagttc tgttagcaac agcggtttac cgcttaatcg caggcgggaa
    21841 cgtcgccccc acagtccggc atcacggcct atctcgataa aatcccgggt tagtgtcgat
    21901 gatgtgaaca gatagcgtcc taacggcgtt ttacccaatt tttgtaacgc tagttccggc
    21961 ccgcttaatg ttgatacagg aacgacggta cgaccggcaa gccacggttc accatcggca
    22021 cataacaaaa tttcacgtaa ccagtaacga gactctttcg gcagtagcgg cagttcttcg
    22081 gggatttcat tctgctcgac aaacccctcg cggatcatcg tcacactcac cgtttttccc
    22141 tgctgttcaa aacgttttgt catggaatcc tccagcaaca gccagtcgaa cagttgcgga
    22201 tccagggcag ggatctcttt aaaatagcgc agcgcacgca gttgcgttaa cgcggggtgt
    22261 gacatgccga actctccgtt acataacgta aaggcattgt atcgcagaaa agtgtttacg
    22321 ggagatgtaa agcaaattaa gtgatctaat gcgcaacaaa aacgcaacaa tcgcatgaag
    22381 tggtaaaacg ctgttgaacc ttacgctttg ccgatgcccg tgctggcgag ggcaaccata
    22441 ggggcatcac cgcccatgat gctaggaacg gtgatgtttt cctttactta aataccttta
    22501 ttctttatac gagtagctcg ccaactcgca gtaaaaattg ggaaaagcgc atatttacca
    22561 tgagatttgg agctgaaaag tgtttcctga gtaagattaa taggcaacat cttatgtctg
    22621 gatttataga tacacgtgga attccaattg gtatcttttg taggattcaa catatcatgt
    22681 aagcgctggg agcttaccac cataacaggc ccatcatcga cagaggagaa caggataaat
    22741 atattatcgt ttcgtgttaa tttattagtg ttaaaggttc ttaacttttc ttcagtttct
    22801 ataaatatag gtgaaacatc atcactacga atccactctt tataaagata ccaggcattt
    22861 gctttgatca tatcatccgt tgatcgtaag gcctgatcga taagttggat aaaaaaattg
    22921 ttatttttat caaccagccc tttattcata ttaaatctgt taataaaatg gatcatctgt
    22981 tgggtatcga aattcacctt actgaatcga tgttgacgca taaaattatc gaacttgtca
    23041 tcttccccag aatgtatata actacgtata attggatctt ttatataagg acaatggcta
    23101 aatgttttct tcatattctc aataaagttt ttattaacaa tattgagaat ttttaattgc
    23161 atcaccagtt ttactgccag aacggatttc aatatcattg cattatctgg caatgtatca
    23221 atcattttta taatgttatt gatataacca tcgagatctt cattcccaaa tatatctgaa
    23281 taatcaatag ttaattccaa ttgttctgta attcaatctt aaaaattccc tgtcgacatt
    23341 cgcacatgat caaacataac agctgtcaaa ttcgatttaa taaatgatat ttctttggca
    23401 gacagaaact ctgctctggt gaagtgcgaa gcgtaaagat aacaattatc gaagtttgaa
    23461 tttctaatct gacattcgcg aaaattacta tgaccaagtt gactctgcga gaagtcaact
    23521 gtatcaagaa tattttggct aaaatcaaca cattcgccac tcgttttgag aaaaatcaca
    23581 ttgtgtaaac gtcgacattt aaatatgaca ttatgaactg ttgccttagt aaaattgcaa
    23641 ttctcaattt ctttacattc gaaagttaca tgttgtagat ttgatgataa aaaagacacc
    23701 ttagaaataa cgtcaccttt aaaatgacaa tttgttaaat caaaatcaga caggtcgata
    23761 tacccctgcg catctttatc catcagaaaa ttacaagcgc attctatttt ttcatttctg
    23821 tagaatttta cacttatagt atcattacta attgagactt cacgtaacat atttattgca
    23881 tgattttttt cgttccgttg ttttatattt aaaaatatat cactgatata aataaagtta
    23941 tctctgttta aacctgtcac cccattgaaa ttacgtagta aatcaagatc tactatatta
    24001 tttccttgca ccacatcatg tttcatcaaa gagttattgc cgccagaatt tgtgatatga
    24061 cctatcataa aacctctgta atacagaaat aaaataacct ggaaaagtag cctgagcgtc
    24121 cagacactac cggcgaaagg aaaagaatct ctgataatgc attgaaataa tggacattct
    24181 tagtgaagaa gaagtgaagt actataaaaa atcgctcata tcccaggaag gaagcataaa
    24241 aaaaggtgcg ccaggagacg caccagttgt tgcaaaatca gcactgtggg gcgtaattac
    24301 cccttgcctt ttacactgct gataaaggtg gaacgggcag atgtcgatcc caggcgttca
    24361 gcttcatcaa gcagttttaa cgccttatca acatcacctt tcgcgacagc gtttttgatc
    24421 gcggtattaa aataactttc cgtgtcgttg agcatcggct cgcttttctt caccggcacc
    24481 ggagcaggtg cagccacagc tggtgccgcc gtgttgccta ccgtaaccgg agctggagcg
    24541 gaagaaccaa atagtggtcc taccaacacg ctggagctgg agttcgtttt gactttcagt
    24601 ttcagtaagc catcgctggt atgacgagca accggatcgg gaatatccgg gatcgagtta
    24661 ccgacgcctt tggcataggc tttagccggg tcgagcagtt gggtcgtctg ctggagatct
    24721 ttttccatgg taaagaccag aacataaagt ttttgctgcc ccaacgccgg tgtcaggcgc
    24781 ataacgcctt ccagccgatc tgcactcatc acgcctagtt cctggtaggt gaaataactg
    24841 ctggggaaga aggctgatgg ggtcatgttc tgatcaataa tcagcacgtt cggcgcaaaa
    24901 acgctggttt gtttgttcac ttcgctggtc agcgtcaggg tcagttcgcc aatgtttgcc
    24961 gggacgctgt acgcagcaac cggaccactg atgccgggaa cgttcagttg ttggccgccg
    25021 gtcgccagtt gggtggtctg gattttagat tgatcgaccg gtgtccaggt gagttgttgc
    25081 agcgcggcag atggaatggc tggcgcgtcg ctggtgtttt gcggtacgta gttaacatcg
    25141 gcaaggctaa ttccaggcgc gcttgccagt aaccctgctg ataaacagag ggcgatgaga
    25201 cttttattca ttttcattgt tttcacctca aaatctggag ctcagcggta gccaggcaat
    25261 agcgcgctaa acccgataat cagaggggct ttcgcccctt tcagataatg acaacctgtt
    25321 tttatgccgg atgcggcgta aacgccttat ccggcccagg ttttgctatt accaccagat
    25381 ttccatctgg gcaccgaagg tccactcgtc gctgtcgcca cgaccgaagc tgccgccgtt
    25441 gaaatcagca ggaacggctt tgccgtagtt cgggttaacc ttgctatcgc cgttgtagtc
    25501 gtaaccccat ttctcatccc acttggcgta ggttgcgaag acacgaatag ccgggcgtga
    25561 ccagatgctg tcgccagcct gccattgttg tgcgagggta attttgtact gattgttctt
    25621 gtcgccggtg cgctgggatt cgacgttgtc gtagccgatt tccatcacgg tgctcatgat
    25681 tggcgtccac ttgtacatcg ggcgaatacc aacggtccac cacttggtgc cgttgtcgtt
    25741 atcccagttg atatcctggt acatacccac gtacatcatg tcccagttgt cgcccatgga
    25801 gatcgcaccg tggtcgagga tacgcagcat gtgaccgttg ttgttgatat tgtaggcaaa
    25861 tttttcgtta tcaaacgcga cgccagaacc ctgcgacaga cctttaccct gcgaggtcat
    25921 cgagtcagta gcgtactgaa caacaaactt gttaaagccc ttcaggacac tctgagtatg
    25981 ttcagcagtg aataaccagc cgtctttcga tgcgccatca accagacgat agttatcacg
    26041 caggttggca cgaccgtagt cgacacccag ttctaatgtg ccgcccgggt tgatttccat
    26101 ctgcgctaaa cgcacatcga aaacgtcgtt cgcggtttcg ttggtatagt cataaatatt
    26161 gttgctggca aaagaggaag aaccaccagc ttcagaggag cgggttgctg ccagagagag
    26221 tttaccgaag ccaacatcga tgttttccag accggcacca ggaccagaaa tatcccagta
    26281 gtagaagtcg atcatatgaa cgtcatgacg ttggtagaag cgcttacctg cccagatggt
    26341 ggaacctggc agccattcga tcaggttttt tccctgcacg tttgcttcac ggaaggccgg
    26401 atcggtagct tcccagtcat tctgttgcgc gacggaatag gccacgttag tgtcgaaata
    26461 gaagctctta tcgccctctt tccacacttc ctgacccaat tttaattcag cataagtttc
    26521 acattcgttg ccaagacggt atttactttg agcaccggta gtctggaaac actgttgttc
    26581 accgccgcta cctgtccagc caataccgga acgtgcatag ccgtggaaat caacagccat
    26641 tgcctgagca gacattacgc ccgctgcgac ggcaaccgcc agaggaagtt tgcgcagagt
    26701 aatcatcatt ctatctcctg agtcattgct tttctttttt cacatcacct gtgacaggct
    26761 ttgtgtgttt tgtggggtgc ttaaacaccc ggctccttat gcagtcgacg acatgcagtg
    26821 ccatcctcac ggaacagatg gcaacgctct ggcggcaggc cgatagcgaa tgtggcacct
    26881 tcttctacca acaccacgtc gttctggcgg tacaccaggt tttgacgaat ggaagggatc
    26941 tggatataga tttgggtttc gttgccgagt tgctcgacga cctgaacttc accctcaagg
    27001 atgacgtcag cgatatcact cggcagtaga tgttccgggc gaatacccag cgacatattg
    27061 gctccaacct ggacatcacg gctttcaaca ggcagccaga cttgttgacg atttggcatc
    27121 ggcagctcca cctgcacttg atcgattgcg gtggcggtca cttttaccgg caggaagttc
    27181 atctttggcg aaccgataaa tccggcgaca aaacggtctg ccggatagtg gtacagttcc
    27241 agcggtttcc caacctgtgc cacgcgaccg gcgtccagca ccacgatttt gtcggccagc
    27301 gtcatcgctt cgacctgatc gtgggtgacg taaatcattg tgcggcccag gcgtttatgc
    27361 agacgggaga tttcgatacg catttgcaca cgcagtgcag catcgaggtt ggagagcggt
    27421 tcatcgagca aaaatacgct tggctcggcc accagcgtac ggccaatcgc cacgcgctga
    27481 cgctgaccac cggagagcgc tttcggtttg cgatccagca aatgcgccag ttgcagcact
    27541 tccgccacct ggttgacgcg ttggttaatc acctcttttt ttgcgccagc cagtttcagg
    27601 ccaaatgaca tgttttctgc tactgacagg tggggataga gcgcgtaaga ctgaaacacc
    27661 ataccaacgc cgcgttctgc tggcggagtg tcattcatcc gtttctcacc gatgaacagg
    27721 tcgccgctgg tgatcgtctc aagcccggca atcatgcgca gtaaagtcga tttaccgcag
    27781 ccagacggtc cgacaaacac cacgaattca ccttcatgga tatcgagatt gatatctttc
    27841 gataccacga cctcgcccca ggctttcgtt acattttgca gctgtacgct cgccatgccc
    27901 ttctcccttt gtaacaacct gtcatcgaca gcaacattca tgatgggctg actatgcgtc
    27961 atcaggagat ggcttaaatc ctccaccccc tggctttttt atgggggagg aggcgggagg
    28021 atgagaacgc ggcttctgtg aactaaaccg aggtcatgta aggaatttcg tgatgttgct
    28081 tgcaaaaatc gtggcgattt tatgtgcgca tccccacatt accgccaatt ctgtaacaga
    28141 gatcacacaa agcaacggtg gggcgtaggg gcaaggagga tggaaagagg ttgccgtata
    28201 aagaaactag agtccgttta ggtgttttca cgagcacttc accaacaagg accatagatt
    28261 atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt
    28321 tccgcctcgg ctctcgccaa aatcgaagaa ggtaaactgg taatctggat taacggcgat
    28381 aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac cggaattaaa
    28441 gtcaccgttg agcatccgga taaactggaa gagaaattcc ctcaggttgc ggcaactggc
    28501 gatggccctg acattatctt ctgggcgcac gaccgctttg gtggctacgc tcaatctggc
    28561 ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc gtttacatgg
    28621 gatgccgtac gttacaacgg caagctgatt gcttacccaa tcgctgttga agcgttatcg
    28681 ctgatttata acaaagacct gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg
    28741 ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg
    28801 tacttcacct ggccgctgat tgctgctgac gggggttatg cgttcaagta tgaaaacggc
    28861 aagtacgaca ttaaagacgt gggcgtggat aacgctggcg cgaaagcggg tctgaccttc
    28921 ctggttgacc tgattaaaaa caaacacatg aatgcagaca ccgattactc catcgcagaa
    28981 gctgccttta ataaaggcga aacagcgatg accatcaacg gcccgtgggc atggtccaac
    29041 atcgacacca gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa gggtcaacca
    29101 tctaaaccgt tcgttggcgt gctgagcacc ggtattaacg ccgccagtcc gaacaaagag
    29161 ctggcgaaag agttcctcga aaactatctg ctgactgatg aaggtctgga agcggttaat
    29221 aaagacaaac cgctgggtgc cgtagcgctg aagtcttacg aggaagagtt ggcgaaagat
    29281 ccacgtattg ccgccaccat ggaaaacgcc cagaaaggtg aaatcatgcc gaacatcccg
    29341 cagatgtccg ctttctggta tgccgtacgt actgcggtga tcaacgccgc cagcggtcgt
    29401 cagactgtcg atgaagccct gaaagacgcg cagactcgta tcaccaagta atgctgtgaa
    29461 atgccggatg cggcgtgaac gccttgtccg gcctacaaaa ctgaaacgca tgtaggcctg
    29521 ataagacgca tcagtgtcgc atcaggcagt tgttgtcgga taaggcgtga aagccttatc
    29581 cgtcctggaa tgaggaagaa ccccatggat gtcattaaaa agaaacattg gtggcaaagc
    29641 gacgcgctga aatggtcagt gctaggtctg ctcggcctgc tggtgggtta ccttgttgtt
    29701 ttaatgtacg cacaagggga atacctgttc gccattacca cgctgatatt gagttcagcg
    29761 gggctgtata ttttcgccaa tcgtaaagcc tacgcctggc gctatgttta cccgggaatg
    29821 gctggaatgg gattattcgt cctcttccct ctggtctgca ccatcgccat tgccttcacc
    29881 aactacagca gcactaacca gctgactttt gaacgtgcgc aggaagtgtt gttagatcgc
    29941 tcctggcaag caggcaaaac ctataacttt ggtctttacc cggcgggcga tgagtggcaa
    30001 ctggcgctca gcgacggcga aaccggcaaa aattacctct ccgacgcgtt taaatttggc
    30061 ggcgagcaaa aactgcaact gaaagaaacg accgcccagc ccgaaggcga acgcgcgaat
    30121 ctgcgcgtga ttacccagaa tcgtcaggcg ctgagtgaca ttaccaccat tctgccggat
    30181 ggcaacaaag tgatgatgag ctccctgcgc cagttttctg gcacgcagcc gctctacaca
    30241 ctcgacggtg acggcacgtt gacgaataat cagagtggcg tgaaatatcg tccgaataac
    30301 caaattggct tttaccagtc cattaccgcc gacggcaact ggggggatga aaagctaagc
    30361 cccggttaca ccgtgaccac cggctggaaa aactttaccc gcgtctttac cgacgaaggc
    30421 attcagaaac cgttcctcgc cattttcgtc tggaccgtgg tgttctcgct gatcactgtc
    30481 tttttaacgg tggcggtcgg catggttctg gcatgtctgg tgcagtggga agcgttgcgc
    30541 ggcaaagcgg tctatcgcgt cctgctgatt ctgccctacg cggtgccatc gttcatttca
    30601 atcttgattt tcaaaggatt gtttaaccag agcttcggtg aaatcaacat gatgttgagc
    30661 gcgctgtttg gcgtgaagcc cgcctggttc agcgatccga ccaccgctcg cacgatgctg
    30721 attatcgtca atacctagct gggttatccg tacatgatga tcctctgcat gggcttgctg
    30781 aaagcgattc cggacgattt gtatgaagcc tcagcaatgg atggcgcagg tccgttccag
    30841 aacttcttta agattacgct gccgctgctg attaaaccgc tgacgccgct gatgatcgcc
    30901 agcttcgcct ttaactttaa caacttcgtg ctgattcagc tgttaaccaa cggcggcccg
    30961 gatcgtcttg gcacgaccac gccagccggt tataccgacc tgcttgttaa ctacacctac
    31021 cgtatcgctt ttgaaggcgg cgggggtcag gacttcggtc tggcggcagc aattgccacg
    31081 ctgatcttcc tgctggtggg tgcgctggcg atagtgaacc tgaaagccac gcgaatgaag
    31141 tttgattaag ggagataaca aaaatggcaa tggtccaacc gaaatcgcaa aaagcacgtt
    31201 tgtttattac tcacctgcta ctgctacttt ttatcgcggc gattatgttc ccgctgctga
    31261 tggtcgtcgc tatctcgctg cgtcagggaa acttcgcgac cggtagcctg atcccggagc
    31321 aaatctcctg ggatcactgg aaactggcgt taggttttag cgttgaacag gctgatggtc
    31381 gcattacgcc accgccattc ccggtactgc tgtggctgtg gaactcggta aaggtcgctg
    31441 ggatttccgc gattggcatt gtggcgctct ccaccacctg cgcctacgct ttcgcccgta
    31501 tgcgctttcc aggcaaagcg acgctgctga aaggaatgct gattttccag atgttcccgg
    31561 cagtactttc actggtcgcg ttgtatgcgt tgtttgatcg tctgggtgag tacattccat
    31621 tcattggcct gaatactcac ggcggcgtaa ttttcgcgta tctgggtggg attgcgctgc
    31681 atgtctggac catcaaaggc tatttcgaaa ccatcgacag ttcgctggaa gaagcggctg
    31741 cgctggatgg tgcgacaccc tggcaggcct tccgccttgt cctgttgccg ctgtcagtac
    31801 cgattctggc ggtggtattc atcctgtcgt ttatcgctgc cattactgaa gttccggtcg
    31861 cgtcgctgtt actgcgtgac gtaaacagct acaccctggc cgtggggatg cagcaatacc
    31921 tcaacccgca aaactacctg tggggtgact ttgccgccgc tgccgtgatg tctgcattac
    31981 cgatcaccat cgtcttcttg ctggctcaac gctggctggt caacggcctg acggcaggtg
    32041 gtgtgaaagg ttaaagatgt tgttctgcca atgttatgcc gctgcaccct caacttacgt
    32101 tatcccaact tgtgactatt attcggcgct ccacggagcg cctttttttc tttcgtctgc
    32161 aatctgaatc gttcgccggt taatattccc atcatagaac ttattatttt tacgttattt
    32221 gttttcccac ttacgataat tctctttcgt gctctgagtc acggcaatag tattgttttc
    32281 atcaattttt gataattatc acaattaaga tcacagaaaa gacattacg
//
LOCUS       DAJTYG010000041        32005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTYG010000041 DAJTYG010000000
VERSION     DAJTYG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.041"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..537
                     /locus_tag="OO568_002001"
     CDS             <1..537
                     /locus_tag="OO568_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641358.1"
                     /translation="VLLTRQGQLVETFISTVEQWRDQKQIITIVCKDGISITDSIITQ
                     FKASKEHTIANGLRIQMTFQGVEFRSIVGQTDISAATGKTATTNDGGATSKKNTGNTT
                     TSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASANERASKVLK
                     SGNALRKYSVNPNKKGTY"
     gene            complement(570..2090)
                     /locus_tag="OO568_002002"
     CDS             complement(570..2090)
                     /locus_tag="OO568_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6641359.1"
                     /translation="MRTVITYLRFSSATQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFNDEGLSGYKGKHLSGQFGDMLSRIESGEFPEGTILLVEAIDRIGRLEHLETEALMN
                     RIIAHGIEIHTLQDGLIYTRDALSDDLGISIIQRVKSYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTRNVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQANNI
                     SSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDTMKRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKGSVTITLQNDTEITIPTDPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            2181..2912
                     /gene="tonB"
                     /locus_tag="OO568_002003"
     CDS             2181..2912
                     /gene="tonB"
                     /locus_tag="OO568_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6641360.1"
                     /translation="MPLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     KPKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRAL
                     SRNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWR
                     YEPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(2952..3350)
                     /gene="yciA"
                     /locus_tag="OO568_002004"
     CDS             complement(2952..3350)
                     /gene="yciA"
                     /locus_tag="OO568_002004"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6641361.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3455..3994)
                     /locus_tag="OO568_002005"
     CDS             complement(3455..3994)
                     /locus_tag="OO568_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6641362.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAVALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(4024..4767)
                     /locus_tag="OO568_002006"
     CDS             complement(4024..4767)
                     /locus_tag="OO568_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6641363.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVCALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(4795..4884)
                     /locus_tag="OO568_002007"
                     /pseudo
     CDS             complement(4795..4884)
                     /locus_tag="OO568_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5124..5762
                     /gene="ompW"
                     /locus_tag="OO568_002008"
     CDS             5124..5762
                     /gene="ompW"
                     /locus_tag="OO568_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6641364.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGS
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(5822..6328)
                     /locus_tag="OO568_002009"
     CDS             complement(5822..6328)
                     /locus_tag="OO568_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6641365.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(6374..6874)
                     /locus_tag="OO568_002010"
     CDS             complement(6374..6874)
                     /locus_tag="OO568_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6641366.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLPRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(6960..7139)
                     /locus_tag="OO568_002011"
     CDS             complement(6960..7139)
                     /locus_tag="OO568_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6641367.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSDKS"
     gene            complement(7520..8326)
                     /gene="trpA"
                     /locus_tag="OO568_002012"
     CDS             complement(7520..8326)
                     /gene="trpA"
                     /locus_tag="OO568_002012"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6641368.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(8326..9519)
                     /gene="trpB"
                     /locus_tag="OO568_002013"
     CDS             complement(8326..9519)
                     /gene="trpB"
                     /locus_tag="OO568_002013"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6641369.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(9531..10892)
                     /gene="trpCF"
                     /locus_tag="OO568_002014"
     CDS             complement(9531..10892)
                     /gene="trpCF"
                     /locus_tag="OO568_002014"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6641370.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(10893..12488)
                     /gene="trpD"
                     /locus_tag="OO568_002015"
     CDS             complement(10893..12488)
                     /gene="trpD"
                     /locus_tag="OO568_002015"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6641371.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(12488..14050)
                     /gene="trpE"
                     /locus_tag="OO568_002016"
     CDS             complement(12488..14050)
                     /gene="trpE"
                     /locus_tag="OO568_002016"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6641372.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALHITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(14072..14164)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            14323..15204
                     /gene="yciV"
                     /locus_tag="OO568_002017"
     CDS             14323..15204
                     /gene="yciV"
                     /locus_tag="OO568_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6641373.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            15201..15821
                     /locus_tag="OO568_002018"
     CDS             15201..15821
                     /locus_tag="OO568_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6641374.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKS
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            15922..16794
                     /gene="rluB"
                     /locus_tag="OO568_002019"
     CDS             15922..16794
                     /gene="rluB"
                     /locus_tag="OO568_002019"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6641375.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(16834..17424)
                     /gene="cobO"
                     /locus_tag="OO568_002020"
     CDS             complement(16834..17424)
                     /gene="cobO"
                     /locus_tag="OO568_002020"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6641376.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(17421..18179)
                     /locus_tag="OO568_002021"
     CDS             complement(17421..18179)
                     /locus_tag="OO568_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6641377.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            18399..19448
                     /gene="sohB"
                     /locus_tag="OO568_002022"
     CDS             18399..19448
                     /gene="sohB"
                     /locus_tag="OO568_002022"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6641378.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(19484..19735)
                     /locus_tag="OO568_002023"
     CDS             complement(19484..19735)
                     /locus_tag="OO568_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6641379.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            20115..22712
                     /gene="topA"
                     /locus_tag="OO568_002024"
     CDS             20115..22712
                     /gene="topA"
                     /locus_tag="OO568_002024"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6641380.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            22922..23896
                     /gene="cysB"
                     /locus_tag="OO568_002025"
     CDS             22922..23896
                     /gene="cysB"
                     /locus_tag="OO568_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6641381.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            24191..24355
                     /locus_tag="OO568_002026"
     CDS             24191..24355
                     /locus_tag="OO568_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6641382.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            24358..24525
                     /locus_tag="OO568_002027"
     CDS             24358..24525
                     /locus_tag="OO568_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641383.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDRQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            24639..24734
                     /locus_tag="OO568_002028"
     CDS             24639..24734
                     /locus_tag="OO568_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641384.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            24898..27573
                     /gene="acnA"
                     /locus_tag="OO568_002029"
     CDS             24898..27573
                     /gene="acnA"
                     /locus_tag="OO568_002029"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6641385.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTQYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(27637..28227)
                     /gene="ribA"
                     /locus_tag="OO568_002030"
     CDS             complement(27637..28227)
                     /gene="ribA"
                     /locus_tag="OO568_002030"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6641386.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            28397..29161
                     /gene="pgpB"
                     /locus_tag="OO568_002031"
     CDS             28397..29161
                     /gene="pgpB"
                     /locus_tag="OO568_002031"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6641387.1"
                     /translation="MRSIARRTAVGAALLFVMPVAVWISGWRWQPGEQSWRLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFSILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            29310..29618
                     /gene="lapA"
                     /locus_tag="OO568_002032"
     CDS             29310..29618
                     /gene="lapA"
                     /locus_tag="OO568_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6641388.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            29625..30794
                     /gene="lapB"
                     /locus_tag="OO568_002033"
     CDS             29625..30794
                     /gene="lapB"
                     /locus_tag="OO568_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6641389.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            30987..31724
                     /gene="pyrF"
                     /locus_tag="OO568_002034"
     CDS             30987..31724
                     /gene="pyrF"
                     /locus_tag="OO568_002034"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6641390.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            31724..>32005
                     /gene="yciH"
                     /locus_tag="OO568_002035"
     CDS             31724..>32005
                     /gene="yciH"
                     /locus_tag="OO568_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6641391.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKS"
BASE COUNT         8039 a         7768 c         8210 g         7988 t
ORIGIN      
        1 gttctattaa ccagacaggg gcaacttgta gagaccttta tttctacggt tgaacaatgg
       61 cgagaccaga agcaaatcat cactattgtt tgtaaagatg gaatatctat cactgatagt
      121 atcattactc agtttaaagc gagtaaagag cataccattg ctaatggtct tcgtattcag
      181 atgaccttcc aaggtgtaga atttcgttca atcgtagggc aaactgatat ttcagcagct
      241 actggcaaga ccgctaccac gaatgatggt ggtgctacca gtaaaaagaa cactggcaac
      301 actacaacga gtcttggaag ccctatgctg acctgtaaag agctttttag ctattcggca
      361 agtgagttat cagatgaagc gctaaaagcc cgtgtaacgt gctctaagag cgttagcgtt
      421 aaaaatggtg agagcacctt cacagcctca gctaatgagc gagcttcaaa agtgcttaag
      481 tcgggtaatg ctttacggaa gtactctgtg aatccgaata agaagggcac ttattgagac
      541 ataaaaaggg gcttcatagc ccctgaaaat tacagttgat aagcatcaat atccagtagc
      601 tctttatcag caatttcaaa gattggtgac ccatcattta gcggtagcgg gtctgtaggt
      661 atggtgattt cagtgtcgtt ctgtagcgtt atagttactg aacccttacc caatgtaagc
      721 ccttttagat gtttggacaa aattaattga cactcgacac gacccgcttt agttgtcagg
      781 tcaatatctt taagctgggg tagttcctta tcagtgagtg aagccttacg cttcatagta
      841 tcaatcgtgg tctgtagttc tgtgcgcttc tggttaagtt ccttaagctg aatttttagt
      901 tcatcaatat catccatctc aagaagtagg gcggtagctt tcttgattct ggctgtgaca
      961 atttcaacct catgctgtaa cgctgcaatc ttcctctctg attctccatc attccccacg
     1021 tcaatcatcc ccagaaaatt atcaatcatc catcggtcaa acggtttacg cttcaacggt
     1081 ggaacatcac agcgaccgtc taagtggtta ttgcacctgt agaccccagc atacttattc
     1141 tttgttccgg taggatggac actagcccca cactgacacc taaacagacc tttgaagatg
     1201 ttaatcgtta gtgggttgtc acttgcaggt gtacggcctt tacgattctt atcaagaatc
     1261 tcttgagctt tattgaacgt tgcagcatct atagcggctt cgtagtagtt cggtatagcg
     1321 gggcggtcat tgcgctgtga tgctggtagt gtacctatcg ttgtctctga ccttaagata
     1381 gtacgcacag aatgaacgct aaatccatta gcatctttac gtcgtgagaa tgaactaatg
     1441 ttattagcct gtaggtgacg tgcaatgtta tgcagacttt caccatctag cagcagcttg
     1501 aaaatcaagc gtacagtttc agcatgttca ttgagtttaa aagtctcagg gtcaatccat
     1561 ccgggaacat tccttgttaa acgttgttca ccagcaaggg caagctttgc acgttgttcc
     1621 catttctgac taacacggaa agacttctgt ttactctcct gatgcgctat gtagctttta
     1681 acccgctgaa tgattgagat tcctaaatca tcggatagag catcccttgt gtagattagc
     1741 ccatcctgta gagtgtgaat ctcgatacca tgagcaataa tgcgattcat caaggcttct
     1801 gtttcaagat gttcaaggcg gcctatgcgg tcgatagctt cgactagtaa aattgtccct
     1861 tctggaaact cccctgattc aatgcgggag agcatatccc caaattgccc tgataagtgt
     1921 ttacctttgt aaccggatag accttcatca ttgaaagatg ccactacctg agcatcgcta
     1981 ttcttcctta accactgttt gaatagctca ttttgtcgtc tggttgaatc agcaccttca
     2041 gcaccttgtg tggctgatga gaatctcaag tatgttatta cggttctcac ggtgataaac
     2101 tcccatgcaa ttgattatca tttaaagatg aacttataaa cgttatggct aatgctacag
     2161 tgccgaatag ctacaataca atgccccttg atttacctcg ccgcttcccc tggccgacgt
     2221 tactttcggt ctgcattcat ggtgctgttg tggcgggtct gctctatacc tcggtacatc
     2281 aggttattga actacctgcg cctgcgcaac caatttctgt cacgatggtt gcgcctgctg
     2341 atctcgaacc gccacaagcc gttcagccgc caccggagcc ggtggtagag ccagaaccgg
     2401 aacctgagcc gatccccgaa ccgccaaaag aagcaccggt ggtcattgaa aagccgaagc
     2461 cgaaacctaa gccaaaaccg aagccaaaac cgaagccggt gaaaaaggta caggagcagc
     2521 caaaacgcga tgtcaaaccc gtagagtcgc gtccggcatc accgtttgaa aatacggcac
     2581 ccgcgcgccc cacatcaagt acggcaacgg ctgcaaccag caagccggtt accagtgtgg
     2641 cttcaggacc acgcgcatta agccgtaatc agccgcagta tccggcacga gcacaggcat
     2701 tgcgcattga agggcaggtt aaagttaaat ttgacgtcac gccggatggt cgcgtggata
     2761 acgtacaaat cctctcagcc aagcctgcga acatgtttga gcgtgaggtg aaaaatgcga
     2821 tgcgcagatg gcgttatgag ccgggtaagc caggcagtgg gattgtggtg aatatcctgt
     2881 ttaaaattaa cggcaccacc gaaattcagt aagcagaaag tcaaaagcct ccgaccggag
     2941 gcttttgact attactcaac aggtaaggcg cgaggttttc cttcaggatc aaccgcgaca
     3001 tacttaaata atgcttctgt cgctttatag cgttgcccga ttggttcaga cgctactttt
     3061 ttcacccaca cttcaatatt aatgctgacc gatgtcgtcc ctttctggac acagcgtgca
     3121 tagcagcaca ccacatcgcc gaccgcaacc ggccgtaaga aagtcattcc ttcaacccgc
     3181 acagtcacta cgcgaccgtg ggcaatttct tttgccagaa tagcgccgcc aatatccatt
     3241 tgtgacatta accaaccacc aaagatgtca ccattggcat tggtatcggc gggcatggct
     3301 aaagtacgta aaacaagatc gccctgaggg acgttatgtg ttgtagacat ggtaaaaccg
     3361 acttaaaagg aaaatcacag gcgcgatgct actatgattt tcctgaggag aacagaggag
     3421 atacagcgcc agccccgaag gactggcagt ctggttagga tttatcttcc tgcggcatgt
     3481 ggcggtagat atagataccg cttaacaatg tgaagattaa ggtaagggcg gtcaggccaa
     3541 agactttaaa gttgacccaa atattttgcg gcagccagaa tgcgatgtag atgtttgcca
     3601 gaccgcaaag gataaagaaa acagcccagg ccagatttag cttcgaccat accggttgcg
     3661 gcagcgtgag ttctttaccc agcatccgct gaattagcgg ctttttcatc acccattggc
     3721 tgactaacag ggctaccgca aacagagcat aaatgactgt aaccttccat ttaataaact
     3781 catcattgtg gaagaacagc gtcaagccac cgaagacgac caccagaaca aaagtgatca
     3841 gggccatctt ctcaacctta cgaaagcgaa cccagctata gataagcaca atcgccgtgg
     3901 cgacgatcag cgctgcagta gccgcataga tgtcataaat cttgtaaaac gcgaaaaaga
     3961 caaccagcgg taaaaaatca agaaactgct tcattttacg attccgtaat caaatgctta
     4021 aggttattgg cgaatcaaca tatacaggcg gaacagatag atgagcaata tggcagaaat
     4081 caggttgctc aaggtgttcg ccagtacggc accaatttcc ggggttaatg cggcaaaaga
     4141 agaggcaaaa agcagcaaca gtgtttttgc cagcaaccag ctcagtactg cgggtgccac
     4201 cagacgcata ttcgcccaag tcagccgcat actgctacgc atcgaggcaa aaatgcccat
     4261 tttgtcctgt accagcatca ccggtgccag ggccagtaaa atggccatga taattcccgg
     4321 aacgaccacc agcatgatgc caatctgtac taaaagggta gtcagaaaaa tcagaataaa
     4381 taacttgggc aatatcggcg cactggcacc aatagctcgc agcgcacaga ctctctgacc
     4441 cgcagacacc agctggataa ttaatattac gcctccggcg agaatggcgt taccgattaa
     4501 ttctgaaaaa gtggacgccg ctgaagcctg cagcaaaatt tgttgctgtt ccggtgacat
     4561 attctgaacc aggtcgaaca acccactact gccgctaacg ggcacgccgt cattgagctg
     4621 cgcaagctgt gcatcactgg gtgagaaaac atgccctaac accactgtga taaacgcaca
     4681 tagcaacgat accaacagaa tggtcataaa ttgattacgg aagaaatttc cggtgtcacg
     4741 gtatacggac tgcgccgtga tagacatgca ctctccttga gttttatgca ggtgttaatt
     4801 agcgggcaat tgtaccctgg ttaagcctcg ggtggcagca tcaggtcttg tatggaaaag
     4861 catatctttg taaagcggag gtaattgctg gccttcaggc gtgggcaaac cgcggcaagt
     4921 ggcgatcgag ttaatatttg cgtagagaaa atatttaaaa attgatttaa atcacattaa
     4981 ccaggattct caatgcaact tctaaattaa tccagatcaa taaatggtga attatcatat
     5041 gtaatgtgat ctatgtagga tcatttgtta ctccaatgta ggtatattcg tcacgttttt
     5101 ataaccataa cgacggagcg gatatgaaaa agttaacagt ggcggctttg gcagtaacaa
     5161 ctcttctttc tggcagtgcc tttgcgcatg aagcaggcga attttttatg cgtgcaggtt
     5221 ctgcaaccgt acgtccaaca gaaggtgctg gtagtacgtt aggaagtctg ggtggattca
     5281 gcgtgaccaa taacactcaa ctgggcttga cgtttactta tatggcgacc gacaacattg
     5341 gtgtggaatt actggcagcg acgccgttcc gccataaaat cggcacccgg gcgaccggcg
     5401 atattgcaac cgttcaccat ctgccgccaa cactgatggc gcagtggtat tttggtgatg
     5461 ccagcagcaa attccgtcct tacgttgggg ccggtattaa ctacaccacc ttctttgata
     5521 atggatttaa cgatcatggc aaagaggcgg ggctttccga tctcagtctg aaagattcct
     5581 ggggagctgc cgggcaggtg ggggttgatt atctgattaa ccgtgactgg ttggttaaca
     5641 tgtcagtgtg gtacatggat atcgatacca ccgccaatta taagctaggc ggtgcacagc
     5701 aacacgatag cgtacgcctc gatccgtggg tgtttatgtt ctcagcagga tatcgttttt
     5761 aattccgcac aaaaacgacc ccgtaatata cggggtcaat aaggacatgg tataaagggt
     5821 attatttctt cgcttctacg ccatcagttt cagaacgaat taaaaatttc tcagttgttt
     5881 gcggaatatg ctgaatcagc cagtcggcca tttgcttttc ctcatttaaa atcgcttcga
     5941 tgatcggaat tgagactgta tcaccggcat tttttgctgc tgctaatagt gaggtgtaac
     6001 aggcgatttc aaattgctcg aagacatatc cgctaataga gcctttaact atttcatcag
     6061 aagggaatat accaccgatt gactgcccaa gcgcagccat tttactcatg gaatctttaa
     6121 tgactgaacg tgaaatgtca ttacgatcaa gaatagtttc cagttgaaca atctggtttt
     6181 tggtttcact aagatgttgt tcaatacgag cgcgtagttc aggataatta tctatacggc
     6241 tggccatgga ttcaagcata gattcggctt gcttttccat tgcgtgggcg tcacgtaacc
     6301 agtcatgata atgttcaata cgattcatga aaatgtcctc tttataaaat gactaaaagt
     6361 taaaattcat atttcaggct ttattttcgg ctttcttatt tacgttatta agggccagat
     6421 cagtcagttt gatgtcggtg gccttttctt cttccagggt ttctttcaga agcttcgctg
     6481 ctttacggta acctaattgt tcagccagcg tcgctaatgt cccgtaactg gcaatctcat
     6541 aatgctcgac tttctgtgct gcggcaatca gtgcggcatc acgcacttcg tttttctcgg
     6601 tactttcgat gacctcatta gcttcttcaa taagaccttc cattgccaca catttcatgc
     6661 gcttaatttt cagatttgat tctgattcca caacttgatc aattcgttca atctgtccat
     6721 gagtttcctc gaggtgcgca tgaaaagcct gacttaattt ttcatttgat gttgctcttg
     6781 cgagttttgc cagtgcccgg ggtaattgtt tttctgcgct gtaggtatct gaaagcagtt
     6841 gaataaatac atcttcaatg gtcttcatat tcatattttt ctccagtgaa atcactgcga
     6901 gccatacggc ccgcagatgc aattatcttt atcagctgat attaataatt aacggtgaat
     6961 caggatttgt ctgatttatt accaccgctt tgttgaccgc cttttttacc cgcttcagat
     7021 gcgcgttgcg gatcattttt aaaattaccg ccgctatgct gaccgccttt acggcctgcg
     7081 tcggatgcct tctcacggtc ttcggcgaaa tttcctgaac caccacgatg ttcggccatg
     7141 ttatttctcc cgttgcgttg cattgtttca ttaatatgag tgttgtgtgt cgacactcat
     7201 taaaattagt cgctaatgag aattagtcaa attaagcgca acgagaagat agagggaaaa
     7261 tatattttga ggaacattct ggatatatta acaattacct gaggaataaa tgacttagag
     7321 aaaattattg attttactgg tgttatgttg cgggattaat ttgtttgatt atattactgt
     7381 tgggcggaaa aatgacgtaa gttgacgttc gaccggggta agcgaaacgg taaaaagata
     7441 aatattaaat gaatttagga tttttccggc ttcattaaag aaagttaaaa tgccgccagc
     7501 ggaactggcg gctgtgggat taactgcgcg tcgccgcttt catcggttgt acaaaaactt
     7561 tcagtgccgc cagcattttc tctggctcat taatatgttg ctcgatgatt ttaacaatgg
     7621 ccgaaccaga aatcgcgccc gcagctcctg catcaatcgc tgcttttacc tgatccgggg
     7681 cggaaatacc aaacccctgc aatggaggtg cagcgttgta ctctttcagc ttcgcaacca
     7741 gatgattgag gggtaacgcg gcgcggtttt ctgcgccggt cacgcccgct cgtgacagca
     7801 aatatgtgta accacgaccg taagaggcta tctggcgcag caggtcgtca tcggcatttg
     7861 gcgggcagat gaagataggt gcgacattat ggcgcaacgc ggcctggcgg aagggcgcgg
     7921 actcttcaac tggcacatcg gcaaccagca ccgaatcgac gccgactttc tcgcactggg
     7981 cataaaactc atcaatgcct ttgttaaaca ccagattggc atacatcaac aggccaatgg
     8041 gaatggtcgg gtgtttctgg cgaatcagtg ccagcatttc aaaacattgt gccggagtca
     8101 cacctgccgc aaaggcgcgc agagtggcgt tttgaatggt cgggccatcc gccagtgggt
     8161 cggagaaggg gatacctaac tccagcgcgt cagcaccggc ttcaattagc gtatcgataa
     8221 ttttcaatga ctgctcaatg cccggatcac cgagcgtgac gaaaggaacg aatgcgcctt
     8281 ctttgcgctc cttcaactgg gcaaacagag attcgtagcg ttccatcaga tttcccctcg
     8341 tgctttcaaa atatcgtgaa cggtgaagat gtctttatcg ccgcgaccgg aaaggttaac
     8401 caccagtagc tgctctttat ccgggttttc gcgcatcatt ttcagcgcat gggccagggc
     8461 gtgggaggat tccagcgccg ggatgatccc ttcgtgcagg cacagcgttt tgaaggcttc
     8521 aagggcttca tcatcggtaa tagacacgta atcagcgcgt ccagtgctgt taagatacgc
     8581 gtgttgtggg ccgacagacg ggaaatccag tccggcggag atggagtaag attcttcaat
     8641 ctgcccgtct tcggtttgca tcatcggcgc tttcataccg aaataaatgc ccacgcgacc
     8701 atgttttagc ggtgcgccgt gctcgccagt ttcgataccg tgaccaccag gctccacacc
     8761 aatcaggccg acgttggttt cattgatgaa atcagcaaac atgccgatgg cattcgaacc
     8821 gccgccaaca caggcgataa cggcatccgg caggcgacct tctctttcca gaatctgcgc
     8881 tttggtttct tcgccaatca tccgctgaaa ctcacggaca atggtcggat aaggatgcgg
     8941 gccagccgcg gtgcccagca tatagtgcgc ggtttcataa ctgccggacc agtcgcgtag
     9001 cgcctcatta caggcatctt tcagcgtggc ggaaccgcta tgtaccggga tcacttccgc
     9061 acccattaag cgcatacgaa aaacgttagg cgactggcgt tcaacgtctt tggcacccat
     9121 ataaatacgg catttcaggc cgagcagggc gctggcaagg gccgacgcca cgccatgctg
     9181 accggccccg gtttcggcga tgatttcggt tttacccatc cgcttcgcca gcaacgcctg
     9241 ccccagcacc tggttggttt tatgtgcgcc gccgtgcagc aaatcttcac gcttgagata
     9301 cagcgtggtg ttcgtcccgg ctgtaatgtt ctggcatttg gtcagtgcgg ttggacgccc
     9361 ggcatagttt ttcagcaggt cgttgaactg ggcttgaaat tcaggatctt tttgcgcgct
     9421 gacaaaagct tcttccagct ggcgcagagc aggcatcagg atttgtggca cgtacatgcc
     9481 gccaaactca ccaaaatagg ggttaagtaa tgttgtcatt gttcctttcc ttaatatgcg
     9541 cgcagcgtct ggaaaaccga ggccaaaaga cgtgcgtctt tgatgcccgg ttgcgactct
     9601 acagcagaat taaaatcaag tccggcgcag ccggtttgtg ccgcttccac gcagttatct
     9661 gcgcctaagc cccccgccag cagaacgttg ccaagcgatt gaccatttaa tagtgaccag
     9721 tcaaaacgtt gcccgcttcc accctggccg ttgtctaaaa catatttatc aacgtgctga
     9781 aactcgcggg cgggcagggt ttcaccgacg cttaatgctt tccagatggc aacatgtgct
     9841 ggcagagctt cacgcagcgt atcgatatac agctgatctt cattaccatg cagttgcact
     9901 gccgccagcg ataacacctt agctttgtcc accacatcgg caatatcgtg attgcggaac
     9961 acgccaacat actgcaacgg tgccgcagcc atcacttcct gcgcctgttc aacgttgacg
    10021 caacgcggtg atgtcgcaac aaaaatcaac ccaccgtaaa tcgcgcccgc gtcataagct
    10081 gctttagcat cttgcccacg cgtcaggcca catactttat tctcacccag caacacccgg
    10141 cgcacggcgg cgtgcaaatc gtcatgggcc atcaacgccg aaccaatcag aaaaccgtta
    10201 gcgaagtggc ttaactcgcg cacctgagcg taagtattga tgccggattc gctgattacc
    10261 gttacgttgt gccccagttt cggcgcaagc tcgcgggtac ggttgagatc aatcgacaaa
    10321 tcacgcagat cgcggttgtt gatgccaacg acctttgccc ctaatgcaat ggcgcgctcc
    10381 agttcctctt cattactgac ttcggtcagc acccccatct ccagactgtg agcgacggct
    10441 gcaagctggc gatattggtc gtcatccagt actgaaagca ttaataagca ggcatcggcc
    10501 tggtaatagc gcgccagata gatctggtaa gggtcgataa tgaagtcttt acataaaatc
    10561 ggctgcgggg cgatttggct gacgatgagg aggaaatcaa agctcccctg aaaatatttc
    10621 tcatcagtca gcaccgaaat tgccgaagcg taatgtttat aaatggcggc aatgcgtgcc
    10681 ggatcgaaat catcacggat cacgcctttt gacggcgacg cttttttaca ctccagaata
    10741 aacgccgtgc gtgcaccctg tagcgcatca taaaaatgtc gtgtgctcgg ctgaacctca
    10801 ttctgaaaac tggccagcgg ttgctgctgt ttgcgggttt ctacccaaat cgccttgtct
    10861 gcgacgattt tcgctaaaac ggtttgcatc atttaccctc gtgcagccag cgcggtaact
    10921 ctgtcgtaag cggaaccact gcgcagtacc tcaagaacgg tttgcgcatt ggcttgcaga
    10981 tcttcatggc catgcaggcg cattaacatg gcgacgttcg cagcgacggc tgcttcatgg
    11041 gcggcgtcgc ctttaccttg taacaagcgt gttaaaatgt cacggttttc ttcaggtgtt
    11101 ccgcctgcca gttgctcctg gtggtaggga gtcaggccaa aatcttcagc ggtcaattga
    11161 tagctcttaa ttttgccgtc atgcagctcg gcaacgattg tcggcgcgtg taatgaaact
    11221 tcatccatcc cgccgctgtg caccaccgcc gcgcgttgat accccagcac acgcaaggtt
    11281 tcggcaatcg gcagcaccag ttccggacta taaacaccaa ttaacgccag cggcggatgc
    11341 gccgggttaa tcaatggccc cagcacattg aacagggtgc gggttttcag ttgctggcga
    11401 accggcatcg cgtggcggaa tccggtgtga tatttcggcg caaagaggaa acatacgcct
    11461 aactcgtcca gcgcctggcg cgatttattg gcgttcatat caagattaat accgaatgcc
    11521 gccagcagat ccgacgagcc ggatttactg gagacgctgc ggttaccgtg tttcgccact
    11581 ttcagcccac aggccgcggc gacaaacgca ctggcggtag aaatattgat actgttgctg
    11641 ccgtcaccgc cagtaccgac gatatcagca aacagataat ccgggcgcgg gaacggcgct
    11701 gcgttttcca gtagcgcggt tgctgccccg gcgatctcgt tcgggtgctc accgcgaatt
    11761 ttcatgctca ccagcgccgc cgccagttgt tccggcttca gctcgccacg caccaccgct
    11821 gaaaacagct ggtggctttc ttgttggcta agcgtctgcg cctgatacag tttttccaga
    11881 atcggttgca gcgtgttggc tggctctagt ttctgctgcg cccaggccag cgtttgttcc
    11941 agcaggcgag cgccctgggt ggtgagaatg gattccggat ggaactggaa tccacaaacg
    12001 cgatccgcat cgtgacgtac tgccatcacc atgccattaa aatgggcgtt gatggttaaa
    12061 ccggccggaa tgttactgcc aaccagcgag tgataacgcg ccaccggcag cgggtttgtt
    12121 aatccggcaa acatcgcctg accgtcatgt tcaatgctcg acgctttacc gtgaagaatt
    12181 tcgcccgcct gaccgacata gcccccgtaa gcttcgacaa ttgcctgatg tccgaggcaa
    12241 atgccaataa tgggcagctt gccacgcaag cgggtgagga gttccggcat acaaccggct
    12301 tcgctcggca caccggggcc aggagaaagc atcagtaccg gattgctcat cgtcgccagg
    12361 cgttcaatta aggtctgcgc cggaatatgg ttgcggtaaa tcaccacgtt atgaccattg
    12421 ctgcgcaact gatctgccag gttgtacgta aaagagtcga tattatcgag cagcagaatg
    12481 tcagccatca gaaagtctcc tgtgcatgat gcgcggtggc aatagcgcgc agtacagcgc
    12541 gggctttgtt acgggtttcg tcggcttccg actgcggaac agaatcaagg actacaccag
    12601 cacccgcttg cacggtggcg ataccgtttt ccaccagcgc cgagcggatc acaatgcagg
    12661 tgtcgagatc gccatgcgcg gtgaaataac ctaccgcgcc gccgtagctg ccgcggcgac
    12721 gaccttccgc ctcggcaatt aactgcatag cgcgtacttt cggcgcaccg cttaacgtcc
    12781 ccatattcat acaggcgcga taagcgtgca gggcgtcaag atcgtgacgc agttcgccga
    12841 ctacgcgaga gacgaggtgc atcacatagg aataacggtc aactttggtg agatcggcga
    12901 cgtagcggct gccaggggtg caaatgcgtg ccagatcatt acgggcgaga tcaaccagca
    12961 tcagatgttc agacagctct ttatggtcag tacgcatttc cagttcaata cggctgtcga
    13021 gatctctgtc cagtgaacca tcggcgcgac gaccgcgtgg gcgtgttccg gcaatcgggt
    13081 agatctcaat ctggcggctg gtggcatcat acttgagcga gctttccggc gacgcgccaa
    13141 atagggtgaa atcattatcc tgcataaaaa acatgtacgg gctgggatta ctctttttca
    13201 gcacgtaata ggccgccagc ggtgacgggc agggcagaga gaaacggcga gatggcacca
    13261 cctggaaaat ttctccagcg cgaatcgctt tttgcaacaa acgcactacg ccaccgaact
    13321 cttcatcgct ctgattacat tcacaacgca tatgcggcac ggaaaccact ggcagcggcg
    13381 gcgcggcttc ggtcagttgc tgacgtagtt cgttcaggcg agcagtgaga cgttgttttt
    13441 cttcttcatt cggagcaaac aggctggcct gaatacgggt gctttttttc tgatggtcaa
    13501 tcaccatcag cgtttcagcg agataaaaac agaaatcagg gcagttattt tccgctgaca
    13561 gttgcggtaa atcttcaaat cccgccacaa ggtcataaga gaacaggccg ccgaagaaca
    13621 ttgcttctcg ttcttccttc ggtacattca acagattctg caataaacgg aaagcgtcaa
    13681 aaaccgaaag ggagcataag cgggcgtctt catccagcag tggactgaca ggggggaagc
    13741 gcagcacacg gcagtttggt gattgttcac tttccacacc cgcaggcagg gcgttatcca
    13801 gtagtgccag cagggcttcg ccattgccgg aaagtgcctg aattgtgacg gtgtcaccta
    13861 atgctgtaat gtgcagcgca ctgtctatca gcagcaggct ttttaaatca tctttgctgt
    13921 cgatatctgc ggattccagc agcagcgttg ccggacgatt cccacacaac tggtgaaaaa
    13981 gcgcagtcgg gttgtcgcga taagcgcctt cgcaggttag cagttcgaga gtcggttttt
    14041 gtgtttgcat tgttattctc taattttgtt caaaaaaaag cccgctcatt aggcgggctg
    14101 ggtatctgat tgctttacgc atggtgaata cactgcccgt ttcaggaagt ggccaccaac
    14161 ctttcagtac gaaaattgct ttcattgtcg ataccatttt tacgtgaact tgcgtactag
    14221 tcaactggtt cgatgattaa ttgtcaacag ctcatttcag aatatttgcc agacccgtta
    14281 tgatgtcggc gcaaaaaaca ttatccagaa cgggagtgcg ccttgagcga cacgaattat
    14341 gcagtgattt acgacctgca cagccatacc acagcttccg atggctgcct gacgccagaa
    14401 gcattggtgc accgtgcagt cgagatgcgc gtcggcaccc tggcgatcac cgaccatgac
    14461 accacagctg caatcgcgcc agcaagagag gaaatttcac gttcgggact ggcgctgaat
    14521 cttattcccg gcgtggaaat ttccacggtc tgggaaaatc atgaaattca tattgtcggg
    14581 ctgaatattg atattactca tccgctaatg tgtgagttcc tcgcgcagca gacagaacgg
    14641 cgcaatcagc gggcgcagca aattgccgaa agacttgaaa aagcgcaaat acctggggcg
    14701 ctggaaggcg cacaacgact ggcgcagggc ggcgcagtga cgcgcggtca ttttgcgcgt
    14761 ttccttgtgg agtgcggcaa agccagttca atggcagatg tctttaaaaa gtatctggcg
    14821 cgcgggaaaa ccggatacgt tccgccacag tggtgtacaa tagaacaagc tattgatgtc
    14881 attcatcatt ctggcggtaa ggcggtgcta gctcatcctg ggcggtacaa tcttagtgct
    14941 aaatggctga aaagattggt tgcgcatttt gccgaacacc acggcgacgc gatggaagtc
    15001 gcgcagtgcc agcaatcgcc caatgaacgc acccagctgg ccacacttgc gcgtcagcat
    15061 catttatggg catcacaagg atctgatttt catcagccat gcccgtggat cgaactgggt
    15121 cgtaaactct ggctgcccgc aggcgttgaa ggcgtctggc agctatggga acagccgcag
    15181 aacaccacag agagggaatt atgagccagt ttttttatat tcatcctgat aacccgcagc
    15241 aacgtctgat caaccaggcg gtggagatcg tgcgtaaagg cggggtgatt gtctatccaa
    15301 ctgattccgg ctatgcgctc ggctgtaaaa gtgaagataa aaacgccatg gagcgtattt
    15361 gtcgtattcg ccagctgccg gacggtcaca actttaccct gatgtgtcgc gatctttctg
    15421 aactctcgac ctattcattc gtggataacg ttgcgtttcg tttgatgaaa aacaacacgc
    15481 cgggcaacta taccttcatc ctgaagggga cgaaggaagt gccacgccgt ctgttgcagg
    15541 agaaacgcaa aaccatcggt atgcgtgtgc cttctaaccc gattgcgcag gcgttgcttg
    15601 aagcactggg cgaaccgatg ctttccactt cgctaatgct gccaggcagc gaatttaccg
    15661 aatccgatcc cgaagaaatt aaagaccgtc tggaaaaaca ggtagatttg attattcacg
    15721 gcggctatct cgggcagaaa ccaacaacgg ttattgatct taccgatgat acgccggtcg
    15781 tggtgcgtga aggcgtaggt gatgtgaagc ctttcttata agtgcgtaac tctgtatact
    15841 acgcggcctt aaaatggcgc tatacggcgc cattgattct cctgttcgac gcctgtgaag
    15901 gcgacaccaa aaggaagctc tatgagcgaa aaattacaga aagtgctggc ccgcgctggc
    15961 cacggctccc gccgtgaaat cgaatctatt attgaagccg gtcgtgtgag tgttgatggc
    16021 aaaattgcta aactcggcga tcgcgttgaa gttacccctg ggctgaaaat tcgtatcgat
    16081 ggtcatctga tttcggtacg tgagtccgct gaacaaattt gtcgcgttct ggcctattac
    16141 aaaccggaag gtgagttgtg tacccgtaac gatccggaag ggcgtccgac ggtgtttgac
    16201 cgtctgccaa agctgcgtgg cgcacgctgg attgccgtag gtcgtctgga cgttaacacc
    16261 tgtggtctgc tgctgttcac caccgatggt gaactggcaa accgtttaat gcatccaagc
    16321 cgtgaagttg aacgtgaata cgccgtgcgt gtatttggtc aggttgacga cgcgaaactg
    16381 cgtgatttga gtcgtggcgt gcaactggaa gatggtccgg cagcttttaa aaccatcaag
    16441 ttcagcggcg gcgaagggat caaccagtgg tacaacgtga ctctgaccga aggccgtaac
    16501 cgtgaagttc gtcgtctgtg ggaagcggtt ggtgtgcagg tgagccgcct gatccgtgtt
    16561 cgttacggtg atatcccact gccaaaaggt ctgccacgcg gtggctggac ggagctggac
    16621 ctcgcccaga ctaactatct gcgcgaactg gtggagcttc cgccagaaac cagctctaaa
    16681 gtcgctgtag aaaaagaccg tcgtcgcatg aaggctaatc agattcgtcg tgcggtgaaa
    16741 cgtcacagtc aggtgagcgg cggtcgtcgt tctggcgggc gtaataacgg ttaatcaaag
    16801 tatgccggac gtcatatccg gcatttttac agattaataa tcaatcccta tttgcgcctt
    16861 aacaccggca tcaaacgcat gtctgatggg gcgcagctca cttaccgtat ccgccagttc
    16921 aagaatatcc cgatgacagc ctcgacccgt gataatcacc gtctgttgat gtggacgttc
    16981 atttaacgcc tgcaccactt cttccagtgg caaatagtca tacgccacca tatacgtcag
    17041 ttcatcaagc aaaaccatat ccagtgagga atcagcaagc atccgctttg catgttgcca
    17101 gacttcgcgg caggcggcgg tatcagactc gcggttttgt gtatcccagg taaagcccgt
    17161 tgccatcacc tgaaactcaa cgccatgtgg ctccagcaga ttgcgttcgc cattaggcca
    17221 ggtgccttta ataaactgca cgacgcccac tttttttccg tgaccaactg cgcgtgttgc
    17281 cgtaccaaat gccgcggtgg ttttgccttt tccattgccg gtaaagacga tgataatacc
    17341 gcgttcatcc tgggcctggg ccacacgagc atctactttt tctttcactc gctgctgacg
    17401 ctgttggtag cgttcatcac tcattgggaa attcctggtt tacggcccgg ttgggcgtca
    17461 aaggtcatgc cggttttacg gcggctgtca tcgcccatca gccagaggta gagcggcatg
    17521 atatcagcgg gtgttttaag tttctgtgga tcttcggtcg ggaaggcgct tgcgcgcatt
    17581 gcggtgcgcg taccgcctgg gttaatgcag ttgacgcgca ggcgctgctg atattcatcg
    17641 gccagtacct gcatcatccc ttcggtggca aatttcgacg ctgcatatgc accccagttg
    17701 gctcgtccct gacgtccaac gcttgatgaa gtaaagacca gtgaaccggc gtccgatttg
    17761 agtaataaag gaagcagtgc ctgggtgagc ataaaggtgg cattaacgtt gacctgcatg
    17821 acgtcctgcc agacctgcgg attttgttcg ctcattgggc aaacatcgcc gagcaatccg
    17881 gcattatgca aaacaccatc cagacgcgga taattaacga caatgcgctg tgccagttgt
    17941 tggcaatttt cggacgtgca ggtcagcaaa tcgagaataa accactgtgg ctgacgccca
    18001 gtttcttcgt ttatgtggct ggctacctga cgtaattttt cttcattacg gcccaacaga
    18061 atcactgtcg caccatagcg tgcatacgtc atcgcggctt cacgaccaat cccatcgctg
    18121 gctcccgtca ccaggataat gcgatcattg agtaaatctt gttttggctg gtaatgcatg
    18181 gctactcctc aacgacgttg tctgtcgcgc tgccagtgta ctttatgact ttggggcttt
    18241 atgcctgaaa cagaagggca tttcaatcag cctggggcga taacaccgca aaatggatac
    18301 tttgtcatac tttcgctgca ataacatctc tgcgagacgg cttaacatgc ctgttgtaaa
    18361 ctgtgagcca aagcgttgtt taaccaaggt ggggactcgt ggaattgttg tctgaatatg
    18421 gtttgttttt ggctaaaatc gttaccgttg tgctagcgat tgcggcgatt gccgccatta
    18481 ttgtcaatgt tgctcaacgt aataaacgcc agcgtggcga gttacgggtc aacaatctca
    18541 gcgaacagta taaggagatg aaagaagaac tggccgcggc gctgatggac tcacatcagc
    18601 aaaaacagtg gcacaaagcg cagaagaaaa agcacaagca agaagcgaaa gcagcaaaag
    18661 cgaaagccaa actgggcgag gtggcaactg acagtaaacc tcgcgtctgg gtgctggatt
    18721 ttaaaggcag catggacgcc catgaagtga actcgctacg tgaagagata acggcggtac
    18781 tcgcagcatt caaaccgcag gatcaggttg tgctccgtct ggaaagccct ggtggcatgg
    18841 tgcatggtta cgggttggcg gcttcgcagc tgcagcgtct gcgtgataaa aacattcctt
    18901 taactgttac ggtagacaaa gtcgctgcca gcggcggtta catgatggcc tgtgtggcgg
    18961 acaaaattgt ttccgcaccg tttgctattg tgggttccat tggggtggtg gcgcaaatgc
    19021 ccaactttaa ccgcttcctg aaaagcaaag atattgatat cgaactgcac accgccgggc
    19081 agtataagcg cacgctgacc ttgctgggtg aaaataccga agaagggcgg gagaaattcc
    19141 gcgaagagct gaacgaaacg catcagttat ttaaagattt tgtgaagcgt atgcgtccgt
    19201 ctctggatat tgaacaggtg gcaacgggtg aacactggta cggacaacag gcggtagaga
    19261 aaggcctggt tgatgaaatc aacaccagtg atgaagttat tcttagcctg atggaaggcc
    19321 gtgaagtggt taatgtacgc tatatgcagc gtaaacgact cattgaccga ttcaccggca
    19381 gcgcggcaga gagcgccgat cgattgttgc tacgctggtg gcagcggggt caaaagccat
    19441 tgatgtaaaa gacaaacgcg aggctaagac ctcgcgtttt gctttaatca accagatgat
    19501 atttttctga aagcacatgg gccaggtgtt tgaacatatt aaacaccgcg gtgcttttgg
    19561 ctgttggcaa tccttgttca tctaaaaagt agtcgccggt aaataccagc acgccattac
    19621 gctgcgtgac gccggtggct tcaatccctg cgagcgtatc ctcatgctca cgaatgattt
    19681 tgttagcctc tttcaacagc gtttcgcggt cgatgggttg tgtctctttg ttcattattt
    19741 actccttaaa caaggacatt agtctacgcc aggcatggct tgcagacaaa tataccacgc
    19801 tggtggcaag agcgccttac tggcaacttt ggattttgca tgctaataaa gttgcgtatc
    19861 ggattttatc aggtacagtg tgacgctttc gtcaatctgg caatagattt gcttgacatt
    19921 cgaccaaaat tccgtcgtgc tatagcgcct gtaggccaag acctgttaac tcagtcacct
    19981 gaattttcgt gaacagagtc acgacaaggg ggtgatatcc gcagagagcg agtccatatc
    20041 ggtaactcgt tgccagtgga aggtttatca acgtgcgacg cattcctgga agaatcaaat
    20101 taggtaaggt gaatatgggt aaagctcttg tcatcgttga gtccccggca aaagccaaaa
    20161 cgatcaacaa gtatctgggt agtgactacg tggtgaaatc cagcgtcggt cacatccgcg
    20221 atttgccgac cagtggctca gctgccaaaa agagtgccga ctctacctcc accaagacgg
    20281 ctaaaaagcc taaaaaggat gaacgtggcg ctctcgtcaa ccgtatgggg gttgacccgt
    20341 ggcacaattg ggaggcgcat tatgaagtgt tgcctggtaa agagaaggtc gtctctgaac
    20401 tgaaacaact ggctgaaaaa gccgaccaca tctatctcgc aaccgacctt gaccgcgaag
    20461 gggaagccat tgcatggcac ctgcgggaag tgattggtgg tgatgatgcg cgctatagcc
    20521 gagtggtgtt taacgaaatt actaaaaacg cgatccgcca ggcatttaac aaaccgggtg
    20581 agctgaatat tgatcgtgtt aatgcccagc aggcgcgtcg ctttatggac cgcgtggtgg
    20641 ggtatatggt ttcgccgctg ctgtggaaaa agatcgctcg tggtctgtct gccggtcgtg
    20701 tgcagtcggt ggcggtccgc ctggtggtag agcgtgagcg tgaaattaaa gcgttcgtac
    20761 cggaagagtt ctgggaagtc gatgccagca cgactacgcc atctggtgaa gcgttggcgt
    20821 tgcaggtgac tcatcagaat gacaaaccgt tccgtccggt caacaaagaa caaactcagg
    20881 ctgcggtaag tctgctggaa aaagcgcgct acagcgtgct ggaacgtgaa gacaaaccga
    20941 caaccagtaa acctggcgct ccttttatta cctctacgct gcaacaagct gccagcaccc
    21001 gtcttggttt tggcgtgaaa aaaaccatga tgatggcgca gcgtttgtat gaagcaggct
    21061 atatcactta catgcgtacc gactccacta acctgagtca ggacgcggta aatatggttc
    21121 gcggttatat cagcgataat tttggtaaga aatatctgcc ggaaagtccg aatcagtacg
    21181 ccagcaaaga aaactcacag gaagcgcacg aagcgattcg tccttctgac gtcaatgtga
    21241 tggcggaatc gctgaaggat atggaagcag atgcgcagaa actgtaccag ctaatctggc
    21301 gtcagttcgt tgcctgccag atgaccccag cgaaatatga ctccacgacg ctgaccgttg
    21361 gtgcgggcga tttccgcctg aaagcacgcg gtcgtatttt gcgctttgat ggctggacaa
    21421 aagtgatgcc tgcactgcgt aaaggcgatg aagatcgtat cttaccggca gtcgataaag
    21481 gcgatgctct gacgctcgtt gaactaacac cagcccagca ctttaccaag ccgccagccc
    21541 gtttcagtga agcatcgctg gttaaagaac tggaaaaacg tggtatcggt cgtccgtcta
    21601 cctatgcgtc gatcatttcg accattcagg atcgtggcta tgtgcgagta gaaaatcgtc
    21661 gtttctatgc ggaaaaaatg ggcgaaatcg tcaccgatcg ccttgaagaa aatttccgcg
    21721 agttaatgaa ctacgacttt accgcgcaga tggaaaacag cctcgaccag gtggcaaatc
    21781 acgaagcaga gtggaaagct gtactggatc acttcttctc ggatttcacc cagcagttag
    21841 ataaagctga aaaagatccg gaagagggtg gtatgcgccc gaaccagatg gttctgacca
    21901 gcattgactg cccgacttgt ggtcgcaaaa tggggattcg cacagcgagc accggggtat
    21961 tccttggctg ttctggctat gcgctgccgc cgaaagagcg ttgcaaaacc accattaacc
    22021 tggtgccgga aaacgaagtg ctgaacgtgc tggaaggcga agatgctgaa accaacgcgc
    22081 tgcgcgcaaa acgtcgttgc ccgaaatgcg gcacggcgat ggacagctat ctcatcgatc
    22141 cgaaacgtaa gttgcatgtc tgtggtaata acccaacctg cgacggttac gagatcgaag
    22201 agggcgaatt ccgcattaaa ggttatgacg gcccgatcgt tgagtgtgaa aaatgtggct
    22261 ctgaaatgca cctgaaaatg gggcgtttcg gtaaatacat ggcctgcacc aacgaagagt
    22321 gtaaaaacac acgtaagatt ttacgtaacg gcgaagtggc accaccgaaa gaagatccgg
    22381 tgccattacc tgagctgccg tgcgaaaaat cagatgctta tttcgtgctg cgtgacggtg
    22441 ctgccggtgt gttcctggct gccaacactt tcccgaaatc gcgtgaaacg cgtgcgccac
    22501 tggtggaaga gctttatcgc ttccgcgacc gtctgccgga aaaactgcgt tatctggccg
    22561 atgcgccaca gcaggatccg gaaggtaata agaccatggt tcgctttagc cgtaaaacca
    22621 aacagcaata tgtctcttcg gaaaaagacg gaaaggcgac tggctggtca gcattttatg
    22681 ttgatggcaa atgggttgaa ggaaaaaaat aacctttaat tctgtcaggt ttttataaac
    22741 aaagggtcgc gaaagcggcc cttttttatg gcatattatt ttttcttcac acctatacac
    22801 taaggctata aatgatatag tggttatagt tagcaccttt tttattatta aatcgtatta
    22861 gtcacccgcc aggtgtgacg aaaaaacgat gttctgatgg cgtctaagtg gatggtttaa
    22921 catgaaatta caacaacttc gctatattgt tgaggtggtc aatcataacc tgaatgtctc
    22981 atcaacagcg gaaggacttt acacatcaca acccgggatc agtaagcaag tcagaatgct
    23041 ggaagacgag ctaggcattc aaattttttc ccgaagcggc aagcacctga cgcaggtaac
    23101 gccagcagga caagaaataa ttcgtatcgc tcgcgaagtc ctgtcgaaag tcgatgccat
    23161 aaaatcggtt gccggtgagc acacctggcc ggataaaggc tcgctgtata tcgccaccac
    23221 gcatacccag gcacgctacg cgttaccaaa cgttatcaaa ggctttattg agcgttatcc
    23281 ccgcgtttct ttgcatatgc accagggccc gccgacacaa attgctgatg ccgtctctaa
    23341 aggcaatgcc gatttcgcta tcgccacaga agcgctgcat ctgtatgaag atttagtgat
    23401 gttaccgtgc taccactgga atcgggctat tgtagtcact ccggatcacc cgctggcagg
    23461 caaaaaagcc attaccattg aagaactggc gcaatatccg ttggtgacat ataccttcgg
    23521 ctttaccgga cgttcagaac tggatactgc ctttaaccgc gcagggttaa cgccgcgtat
    23581 cgttttcacg gcaacggatg ctgacgtcat taaaacttac gtccggttag ggctgggggt
    23641 aggggtcatt gccagcatgg cggtggatcc ggtcgccgat cccgaccttg tgcgcgttga
    23701 tgctcacgat atcttcagcc acagtacaac caaaattggt tttcgccgta gtactttttt
    23761 gcgcagttat atgtatgatt tcattcagcg ttttgcaccg catttaacgc gtgatgtcgt
    23821 tgatgcggct gtcgcattgc gctccaatga agaaattgag gtcatgttta aagatataaa
    23881 actgccggaa aaataatttc ttgcgttatt ttcggcacct tttatgtagc gaaggtgccg
    23941 gaatatattc tcttttgtta cttatttcta taccgtgcac aatattttta tccctcatat
    24001 ttatagggta aattacctgt aaagtagtgt ctaattaccc gcctcgcaaa ttccagtttt
    24061 caaatatcta tttcaggtca aaagattgaa ttattcactg gagacgattc gtaaattcgc
    24121 tggatttttg actaaagttt ctttaggatt tgtctcatcg tatgattaat tacaccaatc
    24181 attcaacgtc ttgctgataa gtgatggcga ttatatgagg ttagcaatgc cttctggaaa
    24241 tcaggaaccc cgccgtgatc ctgaattaaa acgtaaagcc tggctggcgg tttttcttgg
    24301 ttctgcactt ttctgggtgg ttgtcgcact gctgatttgg aaagtgtggg gataactatg
    24361 gtcggtcagg agcaactgga gtcgtcacca ttatgccagc atagtgacaa tgaaccagaa
    24421 acgaaacggg aatgttccgt cgttattcca gacgaccggc aactaacatc gcagcagcaa
    24481 gcctttatag aactgtttgc tgaagatgat cagccgaaac aataattatc atcattctta
    24541 ttacccattt ttaatgaatt aaagggcttt taatacaccg cagcaataac agcttgagtt
    24601 atctcaacac aaaataataa ccgttaaggg tgtagcctat gatcaacaca aatatgaaat
    24661 attggtcctg gatgggcgcg ttttctctgt cgatgctctt ctgggccgaa ctcctctgga
    24721 tcattactca ctgatccttg accccgctgc ggcggggttg tcatttgctt tgccacaagg
    24781 tttctcctct tttatcaatt tgggttgtta tcaaatcgtt acgcgatgtt tgtgttatct
    24841 ttaatattca ccctgaagag aatcagggct tcgcaaccct gtcattaagg aggagctatg
    24901 tcgtcaaccc tacgagaagc cagtaaggac acgttgcagg ccaaagataa aacttaccac
    24961 tactacagcc tgccgcttgc tgctaaatca ctgggcgata tcacccgtct acccaagtca
    25021 ctcaaagttt tgctcgaaaa cctgctgcgc tggcaggatg gtaactcagt taccgaagag
    25081 gatatccacg cactggcggg atggctgaaa aatgcccatg ctgaccgtga aattgcctac
    25141 cgcccggcaa gggtgctgat gcaggacttt accggcgtac ctgccgttgt tgatctggcg
    25201 gcaatgcgcg aagcggttaa acgcctcggt ggcgatactg caaaggttaa cccgctctca
    25261 ccggtcgacc tggtcattga ccactcggtg accgtcgatc gttttggtga tgatgaggca
    25321 tttgaagaaa acgtacgcct ggaaatggaa cgcaaccacg aacgttatgt gttcctgaaa
    25381 tggggaaagc aagcgttcag tcggtttagc gtcgtgccgc caggcacagg catttgccat
    25441 caggttaacc tcgaatatct cggcaaagca gtgtggagcg aattgcagga cggtgaatgg
    25501 attgcttatc cggatacact cgttggtact gactcgcaca ccactatgat caacggcctt
    25561 ggcgtgctgg ggtggggcgt gggtgggatc gaagcagaag ccgccatgtt aggccagccg
    25621 gtttccatgc ttatcccgga cgtagtgggc tttaaactta ccggaaaatt acgtgaaggt
    25681 attaccgcca cagacctggt tctcactgtt acccaaatgc tgcgcaaaca tggcgtggtg
    25741 gggaaattcg tcgaatttta tggtgatggt ctggattcac taccgttggc ggatcgcgcc
    25801 accattgcca atatgtcgcc agaatatggt gccacctgtg gcttcttccc aatcgatgca
    25861 gtaaccctcg attacatgcg tttaagcggg cgcagcgaag atcaggtcga gttggtcgaa
    25921 aaatatgcca aagcgcaggg catgtggcgt aacccgggcg atgaaccgat ttttaccagt
    25981 atgttagaac tggatatgaa tgacgttgaa gcgagcctgg cagggccaaa acgcccacag
    26041 gatcgcgttg cactgcccga tgtaccaaaa gcatttgccg ccagtaacga actggaagtg
    26101 aatgccacgc ataaagatcg cctgccagtc gattatgtta tgaacggaca tcagtatcag
    26161 ttacctgatg gcgctgtggt cattgctgcg ataacctcgt gcaccaacac ctctaaccca
    26221 agtgtgctga tggccgcagg cttgctggcg aaaaaagccg taactcttgg cctcaagcgg
    26281 caaccgtggg tcaaagcgtc gctggcaccg ggttcgaaag tcgtttctga ttatctggca
    26341 aaagcgaaac tgacacagta tctcgacgaa ctggggttta accttgtggg atacggttgt
    26401 accacctgta ttggtaactc tgggccgctg cccgatccta tcgaaacggc aatcaaaaaa
    26461 ggcgatttaa ccgtcggtgc ggtgctgtcc ggcaaccgta actttgaagg ccgtatccat
    26521 ccgctggtta aaactaactg gctggcctcg ccgccgctgg tggttgccta tgcgctggcg
    26581 ggaaatatga atatcaacct ggcttctgag cctatcggcc atgatcgcaa aggcgatccg
    26641 gtttatctga aagatatctg gccatcggca caagaaattg cccgtgcggt agaacaagtc
    26701 tccacagaaa tgttccgcaa agagtacgca gaagtttttg aaggcacagc agagtggaag
    26761 gaaattaacg tcacacgatc cgatacctac ggttggcagg aggactcaac ctatattcgc
    26821 ttatcgcctt tctttgatga aatgcaggca acaccagcac cagtggaaga tattcacggt
    26881 gcgcggatcc tcgcaatgct gggggattca gtcaccactg accatatctc tccggcgggc
    26941 agtattaagc ccgacagccc agcgggtcga tatctacaag gtcggggtgt tgagcgaaaa
    27001 gactttaact cctacggttc gcggcgtggt aaccatgaag tgatgatgcg cggcaccttc
    27061 gccaatattc gcatccgtaa tgaaatggtg cctggcgttg aaggggggat gacgcggcat
    27121 ttacctgaca gcgacgtagt ctctatttat gatgctgcga tgcgctataa gcaggagcaa
    27181 acgccgctgg cggtgattgc cgggaaagag tatggatcag gctcaagccg tgactgggcg
    27241 gcaaaaggac ctcgtctgct tggaattcgt gtggtgattg ccgaatcgtt tgaacgaatt
    27301 caccgttcga atttaattgg catgggcatc ctgccgctgg aatttccgca aggcgtgacg
    27361 cgtaaaacgt tagggctaac tggggaagag aagattgata ttggcgatct gcaaaatcta
    27421 caacctggcg cgacggttcc ggtgacgctt acgcgcgcgg atggtagcca ggaagtcgta
    27481 ccctgccgtt gtcgtatcga caccgcgacg gagttgacct actaccagaa cgacggcatt
    27541 ttgcattatg tcattcgtaa tatgttgaag taacaagtat ttgcttgccg gttattttgc
    27601 ttccggcaag caaatgaatt acacaatgca agagggttat ttgttcagca aatggcccat
    27661 tttctcggct ttggtatcga gataatgttc gttattgggg ttacgaccta caatcaatgg
    27721 tacgcgttca acaatattaa tccctgcttc ggtcagaatt tcgacttttt tcgggttatt
    27781 ggttaacaag cggacttcat tgacgccaag gagtttgaac atatcagcgc aaagagtgaa
    27841 gtcgcgctca tcagcggcga agcctaactg gtggttagcc tctacggtat cgtaaccttg
    27901 atcctgcagt gcgtaagcgc ggattttatt cagcagacca atgttacgac cttcctgacg
    27961 gtgatacagc aaaataccac ggccttcctc ggcaatttgc gtcaatgccg cttcgagctg
    28021 gaagccacaa tcgcagcgca agctgaacag ggcgtcaccg gtcagacatt cggaatggac
    28081 gcgcgcaagt accggggtat gcccggaaat atcgccatag actagcgcga catgatcgtg
    28141 tccggttgcc agttcttcaa atcccaccat caggaaatcg ccccatgggg ttggcagttt
    28201 ggcttctgcc acacgtttaa gctgcatgaa attctccaga taatgctggt tctgtattgg
    28261 cttattttgc cataacgaga aggggttcac ctaatcacga cgcgtcgatc gttcacggaa
    28321 tggcacaaat ctgtcaattt ttcttggaac tggcattttc agttatgatt gtgggactta
    28381 tcaaaaagga gaggccatgc gttcgattgc ccgacgtacc gcagtgggag ctgcactatt
    28441 gtttgtcatg ccagtagccg tatggatttc tggctggcgt tggcaacctg gagaacaaag
    28501 ttggcgacta aaagcggctt tttgggttac tgaaactgtc acccagccct ggggcgtcat
    28561 tacacatttg attttgttcg gctggtttct ctggtgtctg cgttttcgca ttaaggctgc
    28621 ctttgtatta ttttccattc tggcggcggc aatccttgtt ggacaaggcg ttaaatcctg
    28681 gatcaaagac aaagtacagg aaccacgacc ttttgttatc tggttggaaa aaacacatca
    28741 tattccggtt gatgagttct acactttaaa gcgagcagaa cgcggaaatc tagtaaaaga
    28801 acagttggct gaagagaaaa atattccaca atatttgcgt tcacactggc agaaagagac
    28861 ggggtttgcc tttccttccg gtcacacgat gtttgctgcc agttgggcac tgctggccgt
    28921 tggtttgctg tggccgcgtc ggcgaacgtt aaccattgct atcttgttgg tctgggcaac
    28981 gggagtcatg ggaagccgcc tgctactcgg gatgcattgg ccacgcgatc tggtggtagc
    29041 tacgttgatt tcgtgggcgc tggtggcggt ggcaacgtgg cttgcgcaac gaatttgtgg
    29101 gccattaaca ccacctgcgg aagaaaatcg cgaaattgca caacgagaac aagaaagtta
    29161 acgctggttg attttccgaa tttaggcctt aaatcatcaa caatgcgcgt ggatgccatt
    29221 tcgcagacgg cgcgaaaatg gtactttaaa gggctattgc ggtaagttga ccataattta
    29281 ttcgctctaa ccacataacg ggaagtaatg tgaaatattt actcattttc ttactggtgt
    29341 tagcgatctt cgtgatttcg gtcacgttgg gtgcgcagaa cgatcaacag gtgacgttta
    29401 attatctgtt agcgcaaggg gagtaccgta tttccacatt gctggcggta ttgtttgctg
    29461 cggggtttgc tatcggttgg ttgatttgtg gcctgttctg gctgcgagtt cgtgtttccc
    29521 tggcgcgcgc tgaacgtaaa ataaagcgac tggaaaacca gctttcaccc gcgactgacg
    29581 tggctgtagt gccgcactcg tcagcggcga aggaataact ttctatgctg gagttgttgt
    29641 ttctgctttt gcctgtagcc gctgcctatg gctggtatat gggccgcaga agtgcgcaac
    29701 aaaacaagca agatgaagcc aaccgcttgt cgcgtgatta cgtagcgggg gttaacttcc
    29761 tgcttagtaa tcaacaggat aaagcggtag acctgtttct cgatatgctt aaagaggata
    29821 caggcaccgt tgaagcccac cttacgctcg gaaacctgtt ccgttcgcgt ggcgaagttg
    29881 atcgcgctat tcgcatccat cagaccctaa tggaaagcgc ctcgctgacc tatgaacagc
    29941 gtctgttggc gattcaacaa ctggggcgtg attacatggc cgccgggtta tacgaccgcg
    30001 cggaagacat gttcaatcag ctgaccgatg aaactgactt ccgcattggc gcgctgcaac
    30061 agttgctaca aatctaccag gctaccagtg agtggcagaa agcaattgat gttgccgaac
    30121 gcctggtgaa gctgggtaaa gataaacagc gcgtcgaaat tgcccatttc tactgtgagt
    30181 tagccctgca gcatatggcc agcgacgatc tcgatcgtgc catgaccttg ctaaaaaaag
    30241 gggcggcggc agataaaaac agcgcccgcg tatccatcat gatgggacgc gtgtttatgg
    30301 cgaaaggaga atacgccaaa gccgtcgaaa gtttgcaacg cgtcatatcc caggacagag
    30361 aactggtcag cgaaacgctg gaaatgttgc aaacctgcta tcagcagttg ggtaaaactg
    30421 ccgaatgggc agaattcctg caacgcgcgg tggaagagaa caccggtgcc gatgctgaat
    30481 tgatgcttgc tgatatcatc gaagcgcgcg acggtagtga ggctgcacag gtctatatta
    30541 cacgccagct tcagcgtcat ccgaccatgc gtgtattcca taagctaatg gattaccact
    30601 taaatgaagc ggaagaaggg cgcgccaaag agagtctgat ggtgctgcgt gacatggttg
    30661 gcgagaaggt gcgtagtaag cctcgttata gctgccagaa atgtggtttt accgcataca
    30721 ccctctactg gcattgtccg tcttgtcgtg cctggtcaac tattaaaccg attcgcggtc
    30781 ttgatggcct gtaattttta aaaaaatccg actttagtta caacatacta gttattgatg
    30841 tcccattgtg ctccggcaac gacggagcag aaaaagcctg ctaggggaac aatcgcaact
    30901 gttaattttt tatttccacg ggtagaatgc tcgccgttta cctgtttcgc gccacttccg
    30961 gtgcccatca tcaagaaggt ctggtcatga cgttaactgc ttcatcttct tcccgcgctg
    31021 ttacgaattc tcctgtggtt gttgcccttg attatcataa ttgtgatgac gcgctgtcct
    31081 ttgtcgacaa gatcgaccca cgcgattgtc gtctgaaggt cggtaaagag atgtttacat
    31141 tgtttgggcc acagtttgtg cgcgaacttc aacagcgtgg ttttgatatc tttcttgacc
    31201 tgaaattcca cgatatcccc aacactgcag cgcacgctgt cgctgctgca gctgacttag
    31261 gcgtgtggat ggtgaatgtt catgcctccg gtggggcgcg tatgatgacc gcagcgcgtg
    31321 aggcactggt tccgtttggc aaagatgcac cgcttttgat tgctgtgaca gtgttgacca
    31381 gcatggaagc cagcgacctg gccgatcttg gcgtgacact gtcacctgca gattatgcag
    31441 aacgtctggc agcactgacg caaaaatgtg gccttgatgg tgtggtgtgt tctgctcagg
    31501 aagctgtgcg ctttaaacag gtattcggtc aggagttcaa actggttaca ccgggcatcc
    31561 gtccgcaggg gagtgaagct ggtgaccagc gccgcattat gacgccagaa caggcgttgg
    31621 cggctggtgt tgattatatg gtgattggtc gcccggtaac gcaatcggta gatccagcgc
    31681 agacactgaa agcgatcaac gcctctttac agcggagtgc atgatgagtg attccaacag
    31741 ccgtctggtc tactcaacgg aaaccgggcg tattgatgag cccaaagcgg ccccagtacg
    31801 ccctaaaggc gatggagcgg tgcgtattca gcgtcagacc agtggacgta aaggtaaggg
    31861 cgtttgcctg attaccggta tcgatctcga tgatgccgaa ctgacaaaac ttgcagcgga
    31921 actgaagaaa aaatgcggct gcggcggagc agttaaagat ggagttattg aaatccaggg
    31981 cgataagcgt gatttattaa aatca
//
LOCUS       DAJTYG010000042        30358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTYG010000042 DAJTYG010000000
VERSION     DAJTYG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.042"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..373
                     /gene="tuf"
                     /locus_tag="OO568_002036"
     CDS             <1..373
                     /gene="tuf"
                     /locus_tag="OO568_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6641392.1"
                     /translation="GENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKD
                     EGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGL
                     RFAIREGGRTVGAGVVAKVLS"
     gene            603..986
                     /gene="secE"
                     /locus_tag="OO568_002037"
     CDS             603..986
                     /gene="secE"
                     /locus_tag="OO568_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6641393.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            988..1533
                     /gene="nusG"
                     /locus_tag="OO568_002038"
     CDS             988..1533
                     /gene="nusG"
                     /locus_tag="OO568_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6641394.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1692..2120
                     /gene="rplK"
                     /locus_tag="OO568_002039"
     CDS             1692..2120
                     /gene="rplK"
                     /locus_tag="OO568_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6641395.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2124..2828
                     /gene="rplA"
                     /locus_tag="OO568_002040"
     CDS             2124..2828
                     /gene="rplA"
                     /locus_tag="OO568_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6641396.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3241..3738
                     /gene="rplJ"
                     /locus_tag="OO568_002041"
     CDS             3241..3738
                     /gene="rplJ"
                     /locus_tag="OO568_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6641397.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3805..4170
                     /gene="rplL"
                     /locus_tag="OO568_002042"
     CDS             3805..4170
                     /gene="rplL"
                     /locus_tag="OO568_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6641398.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4490..8518
                     /gene="rpoB"
                     /locus_tag="OO568_002043"
     CDS             4490..8518
                     /gene="rpoB"
                     /locus_tag="OO568_002043"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6641399.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EGGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8595..12818
                     /gene="rpoC"
                     /locus_tag="OO568_002044"
     CDS             8595..12818
                     /gene="rpoC"
                     /locus_tag="OO568_002044"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6641400.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13060..14193)
                     /gene="thiH"
                     /locus_tag="OO568_002045"
     CDS             complement(13060..14193)
                     /gene="thiH"
                     /locus_tag="OO568_002045"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6641401.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANNCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14190..14960)
                     /gene="thiG"
                     /locus_tag="OO568_002046"
     CDS             complement(14190..14960)
                     /gene="thiG"
                     /locus_tag="OO568_002046"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6641402.1"
                     /translation="MLRIVDKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14962..15162)
                     /gene="thiS"
                     /locus_tag="OO568_002047"
     CDS             complement(14962..15162)
                     /gene="thiS"
                     /locus_tag="OO568_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6641403.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15146..15901)
                     /gene="thiF"
                     /locus_tag="OO568_002048"
     CDS             complement(15146..15901)
                     /gene="thiF"
                     /locus_tag="OO568_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6641404.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15894..16529)
                     /gene="thiE"
                     /locus_tag="OO568_002049"
     CDS             complement(15894..16529)
                     /gene="thiE"
                     /locus_tag="OO568_002049"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6641405.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKKMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16529..18424)
                     /gene="thiC"
                     /locus_tag="OO568_002050"
     CDS             complement(16529..18424)
                     /gene="thiC"
                     /locus_tag="OO568_002050"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6641406.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18505..18605)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18658..19134)
                     /gene="rsd"
                     /locus_tag="OO568_002051"
     CDS             complement(18658..19134)
                     /gene="rsd"
                     /locus_tag="OO568_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6641407.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19229..20002
                     /gene="nudC"
                     /locus_tag="OO568_002052"
     CDS             19229..20002
                     /gene="nudC"
                     /locus_tag="OO568_002052"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6641408.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20042..21106
                     /gene="hemE"
                     /locus_tag="OO568_002053"
     CDS             20042..21106
                     /gene="hemE"
                     /locus_tag="OO568_002053"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6641409.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21263..21934
                     /gene="nfi"
                     /locus_tag="OO568_002054"
     CDS             21263..21934
                     /gene="nfi"
                     /locus_tag="OO568_002054"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6641410.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21977..22567
                     /locus_tag="OO568_002055"
     CDS             21977..22567
                     /locus_tag="OO568_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6641411.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22754..23026
                     /gene="hupA"
                     /locus_tag="OO568_002056"
     CDS             22754..23026
                     /gene="hupA"
                     /locus_tag="OO568_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6641412.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23039..23734
                     /locus_tag="OO568_002057"
     CDS             23039..23734
                     /locus_tag="OO568_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6641413.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23736..24161)
                     /gene="zraP"
                     /locus_tag="OO568_002058"
     CDS             complement(23736..24161)
                     /gene="zraP"
                     /locus_tag="OO568_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6641414.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24399..25775
                     /gene="zraS"
                     /locus_tag="OO568_002059"
     CDS             24399..25775
                     /gene="zraS"
                     /locus_tag="OO568_002059"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6641415.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25772..27097
                     /gene="zraR"
                     /locus_tag="OO568_002060"
     CDS             25772..27097
                     /gene="zraR"
                     /locus_tag="OO568_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6641416.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDFVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27094..28383)
                     /gene="purD"
                     /locus_tag="OO568_002061"
     CDS             complement(27094..28383)
                     /gene="purD"
                     /locus_tag="OO568_002061"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6641417.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28395..29984)
                     /gene="purH"
                     /locus_tag="OO568_002062"
     CDS             complement(28395..29984)
                     /gene="purH"
                     /locus_tag="OO568_002062"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6641418.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7107 a         7914 c         8351 g         6986 t
ORIGIN      
        1 tggtgagaac gtaggtgttc tgctgcgtgg tatcaaacgt gaagaaatcg aacgtggtca
       61 ggtactggct aagccgggca ccatcaagcc gcacaccaag ttcgaatctg aagtgtacat
      121 tctgtccaaa gatgaaggcg gtcgtcatac tccgttcttc aaaggctacc gtccgcagtt
      181 ctacttccgt actactgacg tgactggtac catcgaactg ccggaaggcg tagagatggt
      241 aatgccgggc gacaacatca aaatggttgt taccctgatc cacccgatcg cgatggacga
      301 cggtctgcgt ttcgcaatcc gtgaaggcgg ccgtaccgtt ggcgcgggcg ttgtagcaaa
      361 agttctgagc taattgccga taacatttga cgcaatgcgc actaaaaggg catcatttga
      421 tgcccttttt gcacgctttc gtaccagaac ctggctcatc agtgattttc tttgtcgtaa
      481 tcattgctga gacaggctct gttgagggcg tataatccga aaagctaata cgcgtttcga
      541 tttggtttgc ctcgcgatcg cggggtgaaa atgtttgtag aaaacttctg acaggttggt
      601 ttatgagtgc gaataccgaa gctcaaggaa gcgggcgcgg cctggaagcg atgaagtggg
      661 tcgttgtggt ggcattgctc ctggcggcga ttgtcggcaa ctacctttat cgcgacatta
      721 tgctgccgct gcgtgcgctg gccgtagtaa ttctgattgc tgcagcgggt ggtgtcgcgc
      781 tgttaacgac aaaaggtaaa gctaccgttg cttttgcccg tgaagcgcgt accgaagtcc
      841 gtaaggtcat ttggccgact cgccaggaaa cattgcacac cacgctgatt gtggctgcgg
      901 ttaccgcagt aatgtcactg atcctgtggg gactggatgg tattctggtt cgcctggtat
      961 cctttatcac tggcctgagg ttctgagatg tctgaagctc ctaaaaagcg ctggtacgtc
     1021 gttcaggcgt tttccggttt tgaaggccgc gtagcaacgt cgctgcgtga gcatatcaaa
     1081 ttacacaaca tggaagattt gtttggtgaa gtcatggtac caaccgaaga agtggttgaa
     1141 atccgtggcg gtcagcgtcg caaaagcgaa cgtaaattct tccctggcta cgtcctcgtt
     1201 cagatggtga tgaacgacgc gagctggcac ttggtgcgca gcgtaccgcg tgtgatgggc
     1261 ttcatcggcg gtacttccga tcgtcctgcg ccaatcagcg ataaagaagt cgatgcgatt
     1321 atgaaccgcc tgcagcaggt tggtgataag ccgcgtccga aaacgctgtt tgaaccgggt
     1381 gaaatggtcc gtgttaatga tggtccgttc gctgacttca acggtgttgt tgaagaagtg
     1441 gattacgaga aatctcgtct gaaagtgtct gtttctatct tcggtcgtgc gaccccggta
     1501 gagctggact tcagccaggt tgaaaaagcc taacccagcg atcaaaaaag cggcgattta
     1561 atcgttgcac aaggcgtgag attggaatac aatttcgcgc cttttgtttt tatgggcctt
     1621 gcccgtaaaa cgatttttta tatcacgggg agcctctcag aggcgttatt acccaacttg
     1681 aggaatttat aatggctaag aaagtacaag cctatgtcaa gctgcaggtt gcagctggta
     1741 tggctaaccc gagtccgcca gtaggtccgg ctctgggtca gcagggcgta aacatcatgg
     1801 aattctgcaa agcgttcaac gcaaaaactg attccatcga aaaaggtctg ccgattccgg
     1861 tagtaatcac cgtttacgct gaccgttctt tcactttcgt taccaagacc ccgccggcag
     1921 cagttctgct gaaaaaagcg gctggtatca agtctggttc cggtaagccg aacaaagaca
     1981 aagtgggtaa aatttcccgc gctcagctgc aggaaatcgc gcagaccaaa gctgccgaca
     2041 tgactggtgc cgacattgaa gcgatgactc gctccatcga aggtactgca cgttccatgg
     2101 gcctggtagt ggaggactaa gaaatggcta aactgaccaa gcgcatgcgt gttatccgcg
     2161 agaaagttga tgcaaccaaa cagtacgaca tcaacgaagc tatcgcactg ctgaaagagc
     2221 tggcgactgc taaattcgta gaaagcgtgg acgtagctgt taacctcggc atcgacgctc
     2281 gtaaatctga ccagaacgta cgtggtgcaa ctgtactgcc gcacggtact ggccgttccg
     2341 ttcgcgtagc cgtatttacc caaggtgcaa acgctgaagc tgctaaagct gcaggcgcag
     2401 aactggtagg tatggaagat ctggctgacc agatcaagaa aggcgaaatg aactttgacg
     2461 ttgttattgc ttctccggat gcaatgcgcg ttgttggcca gctgggccag gttctgggtc
     2521 cgcgcggcct gatgccaaac ccgaaagtgg gtactgtaac accgaacgtt gctgaagcgg
     2581 ttaaaaacgc taaagctggc caggttcgtt accgtaacga caaaaacggc atcatccaca
     2641 ccaccatcgg taaagtggac tttgacgctg acaaactgaa agaaaacctg gaagctctgc
     2701 tggttgcgct gaaaaaagca aaaccgactc aggcgaaagg cgtgtacatc aagaaagtta
     2761 gcatctccac caccatgggt gcaggtgttg cagttgacca ggctggcctg agcgcttctg
     2821 taaactaatg cctttacgtg ggcggtgatt ttgtctacaa tcttaccccc acgtataatg
     2881 cttaatgcag acgtatatcc gagatattcg ggttgtggca aggcggcaac tgagtgagtc
     2941 gccaggagca tagctaacta tgtgactggt gcgaatgaag gaagccaacg ccgccacaag
     3001 ttgaatagcg acggatagaa aagatttgtt cgttggagcc tggcctatcc aggcctccgt
     3061 cgaagaccgc aggagtttcg caagaaactt aatcccctgc gtagacggtg acagaacgct
     3121 aagattattc ttttatattc tggcttgttt ctgctcaccg taattaagac gctctctccg
     3181 tttggaggag tgaagtgagt tccagagatt ttctctggca aacatccagg agcaaagcta
     3241 atggctttaa atcttcaaga caaacaagcg attgttgctg aagtcagcga agtagccaaa
     3301 ggcgcgctgt ctgcagtagt tgcggattcc cgtggcgtaa ctgtagataa aatgactgaa
     3361 ctgcgtaaag caggtcgcga agctggcgta tacatgcgtg ttgttcgtaa caccctgctg
     3421 cgccgtgctg ttgaaggtac tccgttcgag tgcctgaaag acgcgtttgt tggtccgacc
     3481 ctgattgcat actctatgga acacccgggc gctgctgctc gtctgttcaa agagttcgcg
     3541 aaagcgaatg caaaatttga ggtcaaagcc gctgcctttg aaggtgagct gatcccggcg
     3601 tctcagatcg accgcctggc aactctgccg acctacgaag aagcaattgc acgcctgatg
     3661 gcaaccatga aagaagcttc ggctggcaaa ctggttcgta ctctggctgc tgtacgcgat
     3721 gcgaaagaag ctgcttaatc gcagttatct ttttaacgca ttcgcttacg tataaactta
     3781 ttctgatatt caggaacaat ttaaatgtct atcactaaag atcaaatcat tgaagcagtt
     3841 gcagctatgt ctgtaatgga cgttgtagaa ctgatctctg caatggaaga aaaattcggt
     3901 gtttccgctg ctgctgctgt agctgtagct gctggcccgg ttgaagctgc tgaagaaaaa
     3961 actgaattcg acgtaattct gaaagctgct ggcgctaaca aagttgctgt tatcaaagca
     4021 gtacgtggcg caactggcct gggtctgaaa gaagctaaag acctggtaga atctgcaccg
     4081 gctgctctga aagaaggcgt gagcaaagac gacgcagaag cactgaaaaa agctctggaa
     4141 gaagctggcg ctgaagttga agttaaataa gccaaccctt ccggttgcag cctgagaaat
     4201 caggctgatg gctggtgact ttttagtcac cagccttttt gcgctgtaag gcgccagtag
     4261 cgtttcacac tgtttgacta ctgctgtgcc tttcaatgct tgtttctatc gacgacttaa
     4321 tatactgcga caggacgtcc gttctgtgta aatcgcaatg aaatggttta agcgtgatag
     4381 caacaggcat tgcggaaagt gttccatttt ccggtcaaca aaatagtgtt gcacaaactg
     4441 tccgctcaat ggacagatgg gtcgacttgt cagcgagctg aggaacccta tggtttactc
     4501 ctataccgag aaaaaacgta ttcgtaagga ttttggtaaa cgtccacaag ttctggatgt
     4561 accttatctc ctttctatcc agcttgactc gtttcagaaa tttatcgagc aagatcctga
     4621 agggcagtat ggtctggaag ctgctttccg ttctgtattc ccgattcaga gctacagcgg
     4681 taattccgag ctgcaatacg tcagctaccg ccttggcgaa ccggtgtttg acgtccagga
     4741 atgtcaaatc cgtggcgtga cctattccgc accgctgcgc gttaaactgc gtctggtgat
     4801 ctatgagcgc gaagcgccgg aaggcaccgt aaaagacatt aaagaacaag aagtctacat
     4861 gggcgaaatt ccgctcatga cagacaacgg tacctttgtt atcaacggta ctgagcgtgt
     4921 tatcgtttcc cagctgcacc gtagtccggg cgtcttcttt gactccgaca aaggtaaaac
     4981 ccactcttcg ggtaaagtgc tgtataacgc gcgcatcatc ccttaccgtg gttcctggct
     5041 ggacttcgaa ttcgatccga aggacaacct gttcgtacgt atcgaccgtc gccgtaaact
     5101 gcctgcgacc atcattctgc gcgccctgaa ctacaccaca gagcagatcc tcgacctgtt
     5161 ctttgaaaaa gttatctttg aaatccgtga taacaagctg cagatggaac tggtgccgga
     5221 acgcctgcgt ggtgaaaccg catcttttga catcgaagct aacggtaaag tgtacgtaga
     5281 aaaaggccgc cgtatcactg cgcgccacat tcgccagctg gaaaaagacg acgtcaaact
     5341 gatcgaagtc ccggttgagt acatcgcagg taaagtggtt gctaaagact atattgatga
     5401 gtctaccggc gagctgatct gcgcagcgaa catggagctg agcctggatc tgctggctaa
     5461 gctgagccag tctggtcaca agcgtatcga aacgctgttc accaacgatc tggatcacgg
     5521 cccatatatc tctgaaacct tacgtgtcga cccaactaac gaccgtctga gcgcactggt
     5581 agaaatctac cgcatgatgc gccctggcga gccgccgact cgtgaagcag ctgaaagcct
     5641 gttcgagaac ctgttcttct ccgaagaccg ttatgacttg tctgcggttg gtcgtatgaa
     5701 gttcaaccgt tctctgctgc gcgaagaaat cgaaggttcc ggtattctga gcaaagacga
     5761 catcattgat gttatgaaaa agctcatcga tatccgtaac ggtaaaggcg aagtcgatga
     5821 tatcgaccac ctcggcaacc gtcgtatccg ttccgttggc gaaatggcgg aaaaccagtt
     5881 ccgcgttggc ctggtacgtg tagagcgtgc ggtgaaagag cgtctgtctc tgggcgatct
     5941 ggataccctg atgcctcagg atatgatcaa cgccaagccg atttccgcag cagtgaaaga
     6001 gttcttcggt tccagccagc tgtctcagtt tatggaccag aacaacccgc tgtctgagat
     6061 tacgcacaaa cgtcgtatct ccgcactcgg cccaggcggt ctgacccgtg aacgtgcagg
     6121 cttcgaagtt cgagacgtac acccgactca ctacggtcgc gtatgtccaa tcgaaacccc
     6181 tgaaggtccg aacatcggtc tgatcaactc tctgtccgtg tacgcacaga ctaacgaata
     6241 cggcttcctt gagactccgt atcgtaaagt gaccgacggt gttgtaactg acgaaattca
     6301 ctacctgtct gctatcgaag aaggcaacta cgttatcgcc caggcgaact ccaacctgga
     6361 tgaaggaggc cacttcgtag aagacctggt aacttgccgt agcaaaggcg aatccagctt
     6421 gttcagccgc gaccaggttg actacatgga tgtatccacc cagcaggtgg tatccgtcgg
     6481 tgcgtccctg atcccgttcc tggaacacga tgacgccaac cgtgcattga tgggtgcgaa
     6541 catgcaacgt caggccgttc cgactctgcg cgctgataag ccgctggttg gtactggtat
     6601 ggaacgtgct gttgccgttg actccggtgt aactgcggta gctaaacgtg gtggtgtcgt
     6661 tcagtacgtg gatgcttccc gtatcgttat caaagttaac gaagacgaga tgtatccggg
     6721 tgaagcaggt atcgacatct acaacctgac caaatacacc cgttctaacc agaacacctg
     6781 tatcaaccag atgccgtgtg tgtctctggg tgaaccggtt gaacgtggcg acgtgctggc
     6841 agacggtccg tccaccgacc tcggtgaact ggcgcttggt cagaacatgc gcgtagcgtt
     6901 catgccgtgg aatggttaca acttcgaaga ctccatcctc gtatccgagc gtgttgttca
     6961 ggaagaccgt ttcaccacca tccacattca ggaactggcg tgtgtgtccc gtgacaccaa
     7021 gctgggtccg gaagagatca ccgctgacat cccgaacgtg ggtgaagctg cgctctccaa
     7081 actggatgaa tccggtatcg tttacattgg tgcggaagtg accggtggcg acattctggt
     7141 tggtaaggta acgccgaaag gtgaaactca gctgacccca gaagaaaaac tgctgcgtgc
     7201 gatcttcggt gagaaagcct ctgacgttaa agactcttct ctgcgcgtac caaacggtgt
     7261 atccggtacg gttatcgacg ttcaggtctt tactcgcgat ggcgtagaaa aagacaaacg
     7321 tgcgctggaa atcgaagaaa tgcagctcaa acaggcgaag aaagacctgt ctgaagaact
     7381 gcagatcctc gaagcgggtc tgttcagccg tatccgtgct gtgctggtag ccggtggcgt
     7441 tgaagctgag aagctcgaca aactgccgcg cgatcgctgg ctggagctgg gcctgacaga
     7501 cgaagagaaa caaaatcagc tggaacagct ggctgagcag tatgacgaac tgaaacacga
     7561 gttcgagaag aaactcgaag cgaaacgccg aaaaatcacc cagggcgacg atctggcacc
     7621 gggcgtgctg aagattgtta aggtatatct ggcggttaaa cgccgtatcc agcctggtga
     7681 caagatggca ggtcgtcacg gtaacaaggg tgtaatttct aagatcaacc cgatcgaaga
     7741 tatgccttac gatgaaaacg gtacgccggt agacatcgta ctgaacccgc tgggcgtacc
     7801 gtctcgtatg aacatcggtc agatcctcga aacccacctg ggtatggctg cgaaaggtat
     7861 cggcgacaag atcaacgcca tgctgaaaca gcagcaagaa gtcgcgaaac tgcgcgaatt
     7921 catccagcgt gcgtacgatc tgggcgctga cgttcgtcag aaagttgacc tgagtacctt
     7981 cagcgatgaa gaagttatgc gtctggctga aaacctgcgc aaaggtatgc caatcgcaac
     8041 gccggtgttc gacggtgcga aagaagcaga aattaaagag ctgctgaaac ttggcgacct
     8101 gccgacttcc ggtcagatcc gcctgtacga tggtcgcact ggtgaacagt tcgagcgtcc
     8161 ggtaaccgtt ggttacatgt acatgctgaa actgaaccac ctggtcgacg acaagatgca
     8221 cgcgcgttcc accggttctt acagcctggt tactcagcag ccgctgggtg gtaaggcaca
     8281 gttcggtggt cagcgtttcg gggagatgga agtgtgggcg ctggaagcat acggcgcagc
     8341 atacaccctg caggaaatgc tcactgttaa gtctgatgac gtgaacggtc gtaccaagat
     8401 gtataaaaac atcgtggacg gcaaccatca gatggagccg ggcatgccag aatccttcaa
     8461 cgtattgttg aaagagattc gttcgctggg tatcaacatc gaactggaag acgagtaatt
     8521 ctcgctcaaa caggtcactg ctgtcggggt aaaacccggc agcggattgt gctaactccg
     8581 acgggagcaa atccgtgaaa gatttattaa agtttctgaa agcgcagact aaaaccgaag
     8641 agtttgatgc gatcaaaatt gctctggctt cgccagacat gatccgttca tggtctttcg
     8701 gtgaagttaa aaagccggaa accatcaact accgtacgtt caaaccagaa cgtgacggcc
     8761 ttttctgcgc ccgtatcttt gggccggtaa aagattacga gtgcctgtgc ggtaagtaca
     8821 agcgcctgaa acaccgtggc gtcatctgtg agaagtgcgg cgttgaagtg acccagacta
     8881 aagtacgccg tgagcgtatg ggccacatcg aactggcttc cccgactgcg cacatctggt
     8941 tcctgaaatc gctgccgtcc cgtatcggtc tgctgctcga tatgccgctg cgcgatatcg
     9001 aacgcgtact gtactttgaa tcctatgtgg ttatcgaagg cggtatgacc aacctggaac
     9061 gtcagcagat cctgactgaa gagcagtatc tggacgcgct ggaagagttc ggtgacgaat
     9121 tcgacgcgaa gatgggtgcg gaagcaatcc aggctctgct gaagagcatg gatctggagc
     9181 aagagtgcga acagctgcgt gaagagctga acgaaaccaa ctccgaaact aagcgtaaaa
     9241 agctgaccaa gcgtatcaaa ctgctggaag cgttcgttca gtctggtaac aaaccagagt
     9301 ggatgatcct gaccgttctg ccggtactgc cgccagatct gcgtccgctg gttccgctgg
     9361 atggtggtcg tttcgcgact tctgacctga acgatctgta tcgtcgcgtc attaaccgta
     9421 acaaccgtct gaaacgtctg ctggatctgg ctgcgccgga catcatcgta cgtaacgaaa
     9481 aacgtatgct gcaggaagcg gtagacgccc tgctggataa cggtcgtcgc ggtcgtgcga
     9541 tcaccggttc taacaagcgt cctctgaaat ctttggccga catgatcaaa ggtaaacagg
     9601 gtcgtttccg tcagaacctg ctcggtaagc gtgttgacta ctccggtcgt tctgtaatca
     9661 ccgtaggtcc atacctgcgt ctgcatcagt gcggtctgcc gaagaaaatg gcgctggagc
     9721 tgttcaaacc gttcatctac ggcaagctgg aactgcgtgg tcttgctacc accattaaag
     9781 ctgcgaagaa aatggttgag cgcgaagaag ctgtcgtttg ggatatcctg gacgaagtta
     9841 tccgcgaaca cccggtactg ctgaaccgtg caccgactct gcaccgtctg ggtatccagg
     9901 catttgaacc ggtactgatc gaaggtaaag ctatccagct gcacccgctg gtttgtgcgg
     9961 catataacgc cgacttcgat ggtgaccaga tggctgttca cgtaccgctg acgctggaag
    10021 cccagctgga agcgcgtgcg ctgatgatgt ctaccaacaa catcctgtcc ccggcgaacg
    10081 gcgaaccaat catcgttccg tctcaggacg ttgtactggg tctgtactac atgacccgtg
    10141 actgtgttaa cgccaaaggc gaaggcatgg tgctgactgg cccgaaagaa gcagaacgtc
    10201 tgtatcgctc tggtctggct tctctgcatg cgcgcgttaa agtgcgtatc accgagtatg
    10261 aaaaagatgc taacggtgaa ttagtagcga aaaccagcct gaaagacacg actgttggcc
    10321 gtgccattct gtggatgatt gtaccgaaag gtctgcctta ctccatcgtc aaccaggcgc
    10381 tgggtaaaaa agcaatctcc aaaatgctga acacctgcta ccgcattctc ggtctgaaac
    10441 cgaccgttat ttttgcggac cagatcatgt acaccggctt cgcctatgca gcgcgttctg
    10501 gtgcatctgt tggcatcgat gacatggtca tcccggagaa gaaacacgaa atcatctccg
    10561 aggcagaagc agaagttgct gaaattcagg agcagttcca gtctggtctg gtaactgcgg
    10621 gcgaacgcta caacaaagtt atcgatatct gggctgcggc gaacgatcgt gtatccaaag
    10681 cgatgatgga taacctgcaa actgaaaccg ttattaaccg tgacggtcag gaagagaagc
    10741 aggtttcctt caacagcatc tacatgatgg ccgactccgg tgcgcgtggt tctgcggcac
    10801 agattcgtca gcttgctggt atgcgtggtc tgatggcaaa gccggatggc tccatcatcg
    10861 aaacgccaat caccgcgaac ttccgtgaag gtctgaacgt actccagtac ttcatctcca
    10921 cccacggtgc tcgtaaaggt ctggcggata ccgcactgaa aactgcgaac tccggttacc
    10981 tgactcgtcg tctggttgac gtggcgcagg acctggtggt taccgaagac gattgtggta
    11041 cccatgaagg tatcatgatg actccggtta tcgagggtgg tgacgttaaa gagccgctgc
    11101 gcgatcgcgt actgggtcgt gtaactgctg aagacgttct gaagccgggt actgctgata
    11161 tcctcgttcc gcgcaacacg ctgctgcacg aacagtggtg tgacctgctg gaagagaact
    11221 ctgtcgacgc ggtaaaagta cgttctgttg tatcttgtga caccgacttt ggtgtatgtg
    11281 cgcactgcta cggtcgtgac ctggcgcgtg gccacatcat caacaaaggt gaagcaatcg
    11341 gtgttatcgc ggcacagtcc atcggtgaac cgggtacaca gctgaccatg cgtacgttcc
    11401 acatcggtgg tgcggcatct cgtgcggctg ctgaatccag catccaggtg aaaaacaaag
    11461 gtagcatcaa gctcagcaac gtgaagtcgg ttgtgaactc cagcggtaaa ctggttatca
    11521 cttcccgtaa caccgaactg aaactgatcg acgaattcgg tcgtaccaaa gaaagctaca
    11581 aagtgcctta cggtgcggta ctggcgaaag gcgatggcga acaggttgct ggcggcgaaa
    11641 ccgttgcaaa ctgggacccg cacaccatgc cggttatcac cgaagtaagc ggttttgtac
    11701 gctttactga catgatcgac ggccagacca ttactcgtca gaccgacgaa ctgaccggtc
    11761 tgtcttcgct ggtggttctg gattccgcag aacgtaccgc aggtggtaaa gatctgcgtc
    11821 cggcactgaa aatcgttgat gctcagggta acgacgttct gatcccaggt accgatatgc
    11881 ctgcgcagta cttcctgccg ggtaaagcga ttgttcagct ggaagatggc gtacagatca
    11941 gctctggtga caccttggcg cgtattccgc aggaatccgg cggtaccaag gacatcaccg
    12001 gtggtctgcc gcgcgttgcg gacctgttcg aagctcgtcg tccgaaagaa ccggcaatcc
    12061 tggctgaaat cagcggtatc gtttccttcg gtaaagaaac caaaggtaaa cgtcgtctgg
    12121 ttatcacccc ggtagacggt agcgatccgt acgaagagat gattccgaaa tggcgtcagc
    12181 tcaacgtgtt cgaaggtgaa cgtgtagaac gtggtgacgt aatttccgac ggtccggaag
    12241 cgccgcacga cattctgcgt ctgcgtggtg ttcatgctgt gactcgttac atcgttaacg
    12301 aagtacagga cgtataccgt ctgcagggcg taaagattaa cgataaacac atcgaagtta
    12361 tcgttcgtca gatgctgcgt aaagctacca tcgttaacgc gggcagctcc gacttcctgg
    12421 aaggcgaaca ggttgaatac tctcgcgtca agatcgcaaa ccgcgaactg gaagcgaacg
    12481 gcaaagtggg tgcaacttac tcccgcgatc tgctgggtat caccaaagcg tctctggcaa
    12541 ccgagtcctt catctccgcg gcatcgttcc aggagaccac tcgcgtgctg accgaagcag
    12601 ccgttgcggg caaacgcgac gaactgcgcg gcctgaaaga gaacgttatc gtgggtcgtc
    12661 tgatcccggc aggtaccggt tacgcgtacc accaggatcg tatgcgtcgc cgtgctgcgg
    12721 gtgaagctcc ggctgcaccg caggtgactg cagaagacgc atctgccagc ctggcagaac
    12781 tgctgaacgc aggtctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta
    12841 aaaacccgct tcggcgggtt tttttatggg tgttattcag agggcaaatt cttcacggaa
    12901 aatattaaga aaggcagggc gaaacgagga agaagctttt cgccctggac aacattcctg
    12961 ctgacggcac taccataaaa aaacacaacg ctttcaacaa ccttgcgttt ttcatcttcc
    13021 gtagcggctc ggaataaatt tacgttctta cattccgtct catagtcttt gcgaggcgcg
    13081 tcccagatag ctgtcccagt ctttccatac cggctgcaaa ccctgagcgg ttaacgcggc
    13141 agcaaccgct tccggtctgc gatcgtcgtg cggtgagaac tgttccaact cggggtgatt
    13201 atcggcatag ccacctggct gcgttttcga gaaggcgctg acgttattga tcgccagcgg
    13261 aataacgcga tcgcgaaacc acggtgattc ccgcgtggag agtgacagtt caatctccgg
    13321 tgcaagcagt cggaaggcgc agatggtttg cactaactgg cgttcatcca taatcgacgc
    13381 aggctcaatg ccgccagtac acgggcgcag gcgcggaaag gagacagagt aacggctttg
    13441 ccagtaatgc tgttgcagcc atagcaaatg ttcggcaacc atatagcagt caacgcgcca
    13501 gttgtcggaa aggccaatta gcgcgccgag gcctatctta tcaatccccg cacgccccag
    13561 ccgatccggc gtttccagcc gccagaagaa gtcctgtttt ttgcctttca aatgatggcg
    13621 ggtataagtc gcctcgtgat atgtctcctg ataaaccatc accccatcca gaccaagttg
    13681 ctttaactcg gcgtattccg tctccgccag cggttgcact tccatttgta gtgaagagaa
    13741 ctgttcacgc agggcgggga gatgacgacg aaagtaatcc atccccactt tcgcctgatg
    13801 ttcaccagtg actaatagca gatgttcaaa gcccatctcc cgtatagcgg cgctttccct
    13861 ggcaatatcc gcttcatcca gcgttttgcg cttgatgcga ttactcatgg aaaatccgca
    13921 gtacgtgcag ttgttagcgc aaagattgga aagataaagc gggacgtaga aactaactgt
    13981 gttgccaaat cgctgacggg tcagacgctg cgcctgttgg gccagttgtt ccagatagcc
    14041 actggcggca ggcgataaca gcgccatcat gtcgtcgcgg gtgagttgcg aggcatttag
    14101 cgcccgctct acgtcagcag ccgttttgcc gttgatacgc aggcggatgt cgtcccagtc
    14161 cagttgtcgc cagcgattgc tgaaggtttt catgccgatg cctccagaaa tccggtcagc
    14221 gggctggtgg catgagcaaa ataactgcgg ctgcccggtc cggactgacg tgccagcagg
    14281 cctgcttcta ccgccagacg aaatgccttc gccatgttga cgggatcgtc cgcgacggca
    14341 atcgccgtat tcactaacac cgcgtcggcc cccatttcca gcgcctgcgc ggcatggctg
    14401 ggaacgccga tgccagcatc gacaaccacc ggaactgtgg cctgctggat aataatctcc
    14461 agcatggcac gggtttccag tccctgattc gagccaatcg gcgcgccgag cggcatcacc
    14521 gctgcacagc cgacttcttc cagacgttta cacaataccg gatcggcccc gcagtaaggc
    14581 agcacgacaa atccctgttg taccagcgtt tcggcggctt tcagggtttc gatgggatcg
    14641 ggcaacagcc agcgggcgtc agggtgaatc tccaatttta gccagtttgt gcctaacgct
    14701 tcacgtgcca gatgggcggc gaaaatggct tcttccgctg ttttcgcccc ggaagtattc
    14761 ggcagcaggg ccacacccgc cgcgataagc ggttcgagga tagcgtcgtt gtgctggcgc
    14821 aagtcgacac gtttcatcgc cagtgtcacc agctggctgc cggaagcgcg gatcgcctcc
    14881 accatcagtt gtgaagaagc gaatttccct gtgccggtaa acagatgtga atcaaacgtt
    14941 ttgtccacaa tacgtaacat ctcaaccccc tgcaataacc tgaaaaagca ggatctggtc
    15001 gccatcctgc acgatatgtt gcgtccactg ctcacgcggg acgatttgct gattaatcgc
    15061 cagagccgcg cccgcttgtc gttggtcgag ttgctccagt agttcgtgaa cagtttgccc
    15121 ggcggcgcac tgcatcggtt gatcgttaaa caggatctgc attgcttcct ccgcataccg
    15181 ggcaaccact ggcgcggcgc aacgccaggc tgcgccactg gctcgactta ccgtcgaaca
    15241 gtcggagttc tcccgcaggc gtctctatac cgcttaataa cttaatggct tccagtgcct
    15301 gcaaagtgcc catcaccccg accaccgggc caaccacgcc cgccgtgcgg cagttgcgtt
    15361 ctggctgctg gttatctggc cacaggcagc ggtaacaccc ctgctcccag ggcggtgtca
    15421 gtaccatcaa ctgaccgcca aatccgaccg cgctggcggt gataagcggc gtgttgagtg
    15481 ccacgcaggc ggcattaatc tcctggcgag tcgccatatt gtcggtacag tcgagcacca
    15541 catcggcttg tgcaaccgca tctttcaacg cctcacccgt taaccgttgt tgtaatgctg
    15601 tcagttgaat gtcgggattc aactgtgtca gtcgctgttg gctgacctgc gatttcgggc
    15661 tgttgatatc ttcagtggta aagaggattt gtcgttgcag attgcttaaa tgcacatcgt
    15721 cgtcatctgc cagtaccagc gtcccgacgc cagcccctgc caggtatagc gcagcaggtg
    15781 tacccagccc gccaagacca ataatcagca cctggctgtc gagcagtttt tgctgcccgt
    15841 caagagcgat atcgtcgagc agaatttggc ggctataacg cataaagtca cggtcattca
    15901 tcgccaactc ctgcaatttc cagcaactgt gccgttgcca aacgccagtc tgcggcttga
    15961 gtaatggcgc tgacgacggc gatactgccg acacccgttg ctatcaccgc aggcgcgcgt
    16021 gccagactga taccgccaat cgccacggtg ggataatccg ccaggcgctc aacatgccgt
    16081 gccaactgtt ccagcccctg cggtgcagaa ggcatctttt tggtttgcgt cgggaacaca
    16141 tgtcccagcg cgatataaga ggggcgtgct gccagcgcga cgtcgatttc catatcgtca
    16201 tgtgtcgaaa cgcccagccg caggcctgcc gcgcggatgg cattgagatc ggtggcttgc
    16261 aaatcttcct gccccaaatg gacgccatac gcctgatgct tgatcgcgag ccgccagtaa
    16321 tcgttgataa acaatcgcgc gttatagcgg cggcccagcg caattgccgc cacgacatcg
    16381 gcttccacct cttcatcgcg ccgatctttg atgcgtagct ggagagtacg tacgcctgca
    16441 tccaacagac gttcgatcca ctgtacgctg tccaccaccg ggtacagtcc taaacgaaaa
    16501 ggtacaggag gaaaatcagg ctgatacatc acgcttcctc cttatgcagg tagatttctc
    16561 cgcctctggc acggaagttc tccgacatat ccgccattcc cacttcaatg gtttgcgcgg
    16621 cggcgtaatc gcgtacttcc tggctgattt tcatcgagca gaattttggc ccacacatgg
    16681 agcaaaaatg ggcgactttg ccggactctt ggggcagggt ttcatcgtga taagcgcggg
    16741 cggtaaacgg gtcgagggcc agattaaact ggtcttccca gcgaaattcg aagcgggctt
    16801 tcgacatggc gttatcgcga atttgcgcgc ccggatgccc ttttgccagg tcagcagcgt
    16861 gggcggcaat cttataagtg ataagcccct gcttaacatc ttctttattg ggcagaccca
    16921 gatgctcttt tggcgttacg taacagagca tcgcgcagcc aaaccagcca atcatcgccg
    16981 caccaatccc cgacgtgaag tggtcatagc ccggtgcaat atcggtagtt agcggcccca
    17041 gagtgtaaaa cggcgcttcg tggcagtgct ctaactcctc ggtcatattg cggcggatca
    17101 tctgcatcgg cacatggcct gggccttcaa tcatcacctg tacgtcatat tcccaggcga
    17161 ttttggtcag ttcgcccagc gtatgcaatt cggcaaactg cgcttcatcg ttggcgtcct
    17221 gaatagaacc ggggcgcaga ccgtcgccca gcgacagcga aacgtcataa gcggcacaga
    17281 tttcacaaat ttcgcggaag tgttgataga ggaaattttc ctgatgatgg gagaggcacc
    17341 atttcgccat aatcgaaccg ccgcgagaga cgataccggt caggcgtttc gcggtcatcg
    17401 gcacatagcg cagcagtacg cccgcatgga tagtgaagta atccacacct tgctcggcct
    17461 gttccagcag cgtgtcgcgg aacgcttccc aggtaagatc ttcggcgatc ccgttaacct
    17521 tctccagcgc ctggtagatc ggcactgtac cgatcggcac cggactgtta cgcaaaatcc
    17581 actcgcgggt ttcgtgaata tagcgaccgg tggagagatc catcaccgta tccgctcccc
    17641 agcgcgtgga ccataccagc ttttccactt cttcttcgat ggaagaggtg accgccgagt
    17701 tgccgatatt ggcgttaact tttaccagga agttacggcc aataatcatc ggctccgatt
    17761 ccggatgatt aatgttggcc gggataatcg cacgtccggc agcaacttca tcacggacaa
    17821 attccgcagt gatattttcc ggcagatgtg cgccaaagct cattcccgga tgctggtggc
    17881 gtaaaacctc gctacggatg cgctcgcggc ccatattctc gcggatggcg atgaattcca
    17941 tttccggcgt gatgatgccc tggcgggcgt agtgcagttg ggtgacacgg cgtcctgttt
    18001 tggcgcgttt tggcgttaat aagccgctaa aacgtagctc gtcgagacca tcatctgcca
    18061 gccgcgttct ggtgtaatcg gaactgcgca cggtaagttc ttcggtatcg gcgcgcgcat
    18121 cgatccacgg ctggcgtagt tttgccagcc cttgctgcac gttaatggcg atctgcggat
    18181 caccatacgg gccggaggtg tcgtagaccg gaatcgcttc gttttcttcg tactgcggct
    18241 gttctttgct accgccaatt agcgtcgggc taagctggat ctcacgcatc ggcacgcgca
    18301 cgccggggtg tgtgccagtg atataaatgc gttttgagtt gggaaaggcg gtgccttcca
    18361 gggtgtcgat aaaatgttgg gcccgggcgc gttgttcgcg gcgggtcagt tttgttgcag
    18421 acatagctca ttccaaaaag ttaaggacgt ggcttgtcag acgacggatg aagcaagaga
    18481 cgatcgccgc aaaggcgatg cgatagcaga ttaactcttg ttcccttcgc aggtattagc
    18541 ctgatcaggt tccgcggatc ccgaataaac ggtctcagcc agttaaggca ctccgacaag
    18601 aaattagccc ccacaaacgg ggcgttgaat gtaaattacg cgttaacagc gcagaactca
    18661 agcaggatgt ttgacgcggg cggcgtcaag caccagcaga atcagcttat cttccagcac
    18721 aaagcgcgct tccagcgcct cgccgatgtc agataaaacc tgttgaaact caaggtaatt
    18781 atcatgatcg atggcggttt ccaggctgga atcgtagtaa tccataatct gttgggtatt
    18841 ggcttcgagt tgcggccaaa tcttcgcggc gcgcgcgagt tgcccgttgc cttccagctt
    18901 atgaagaatg cgctcataaa tactgaaatg tccggcagac aagtaatcga ccaggctctg
    18961 acaaaaatca tcaagggctt tttcgtttag cctcatgtac gattctttgc caggcttaat
    19021 gccaaccaga ttgtagtaag ccacgagcag atgcttacgt acatgtagcc agcgatcaac
    19081 cagtttgtta cttcctctga cgcgctccgt caggttatcg agctggttaa gcatgattga
    19141 ctccgcaagt ttgtattcaa aaactgctca gtgagaaatg taaaaaccat gttaaacatg
    19201 ccagtgatgc aaaggtagtg caagagctat ggatcgtata attgaaaaat tagatcacgg
    19261 ctggtgggtc gtcagccatg aacaaaaatt atggttgccg aagggagaat tgccatatgg
    19321 cgaagcggca aatttcgatc ttgtgggtca gcgcgcacta caaatcggcg aatggcaggg
    19381 ggaacctgtt tggttaatac aacagcagcg gcgtcacgat atggggtcgg tacgtcaggt
    19441 cattgatctc gatgttgggc tgtttcaact ggccggacgt ggcgtgcaac tggcggagtt
    19501 ttaccgatcg cataaatact gtggttactg cgggcatgaa atgtatccga gcaaaaccga
    19561 atgggcgatg ctgtgcagcc attgccgtga gcgttactac ccgcaaatcg ccccctgcat
    19621 tattgttgcc atccgtcgcg atgattcgat cctcctcgcc cagcataccc gccatcgtaa
    19681 cggtgtccat acagtacttg ccggattcgt cgaagtgggc gaaaccctcg agcaggcagt
    19741 cgcgcgggaa gtgatggaag agagcggaat taaagttaaa aacttgcgtt acgtgacttc
    19801 tcagccgtgg ccgtttcctc agtctttaat gaccgcgttt atggcggaat atgacagcgg
    19861 cgacatcgtg atcgacccga aagaattgct cgaggcgaac tggtatcgct atgacgattt
    19921 gccgttactc ccgccgcccg gcaccgtagc gcgccgtctg atagaagata ctgtggcgat
    19981 gtgtcgggca gagtatgagt gatgatacac tgaccgcctg acgcactaag gaacagccaa
    20041 aatgaccgaa cttaaaaacg atcgttatct gcgggcgctg ctgcgccagc ccgttgatgt
    20101 cactccagta tggatgatgc gccaggcggg tcgctatcta ccggaatata aagccacgcg
    20161 cgcccaggcg ggcgatttta tgtcgctgtg caaaaacgcc gagcttgcgt gcgaagtgac
    20221 tttgcaaccg ctgcgtcgct acccgctgga tgcggcgatc ctcttttccg atatcctcac
    20281 cgtgccggac gcgatggggt tagggctcta ttttgaagcc ggagaaggtc cgcgttttac
    20341 ctcgccagtc acctgcaaag ccgacgtcga taaactgcca attccggacc cggaagatga
    20401 gctgggttac gtgatgaacg cggtgcgtac cattcgtcac gaactgaaag gcgaagtgcc
    20461 gctgattggt ttttccggca gcccgtggac gctggcaacc tacatggtgg aaggcggcag
    20521 cagcaaagcc ttcaccgtga tcaaaaaaat gatgtatgcc gatccgcagg cgctgcacgc
    20581 tctgctcgat aaactggcga aaagcgtcac tttgtatctg aatgcgcaga ttaaagccgg
    20641 tgctcaggca gtgatgattt tcgacacctg gggcggcgtg cttaccgggc gcgattatca
    20701 acagttctcg ctctattaca tgcataaaat tgttgatggt ttactgcgtg aaaacgacgg
    20761 tcgccgcgtg ccagtcacgc tgtttaccaa aggcggcgga cagtggctgg aagccatggc
    20821 tgaaaccggt tgcgatgcgc tgggcctcga ctggacaacg gacatcgccg atgcgcgccg
    20881 ccgtgtgggc aataaagtcg cgttgcaggg taatatggat ccgtcgatgc tgtacgcgcc
    20941 gcctgcccgc attgaagaag aagtagcgac tatacttgca ggtttcggtc acggcgaagg
    21001 tcatgtcttt aaccttggtc acggcattca tcaggatgtg ccgccagaac atgctggcgt
    21061 gttcgtggag gcagtgcatc gactgtctga acagtatcac cgctaagagc ctgatccatc
    21121 aggctatttt atttgccatt ttgaggccgg gcagtgctca aaatcctcac gtactacgtg
    21181 tacgctccgg tttttccgcg ctgtccggcc tcaaactgcc ttcaacaatt acgcctgttg
    21241 aaccaggctc taaggagcga ttatggatct cgcgtcatta cgcgctcaac aaatcgaact
    21301 ggcttcttct gtgatctgcg aggatcgact cgataaagat ccaccggatc tgatcgccgg
    21361 agccgatgtc gggtttgagc agggcggaga agtgacgcga gcggcgatgg tgctgctgaa
    21421 atatccctcg cttgagctgg tggagtataa agttgcccgc atcgccacca ccatgcctta
    21481 cattccaggt tttctttcct tccgcgaata tcctgcgctg ctggcagcgt gggagatgct
    21541 gtcgcaaaag ccggatttag tgtttgtcga tggtcatggg atctcgcatc ctcgccgtct
    21601 tggcgtcgcc agccattttg gcttattggt ggatgtgccg accattggcg tggcgaaaaa
    21661 acggctctgc ggtaaattcg aaccgctctc cagcaaaccg ggcgcgctgg ccccactgat
    21721 ggataaaggc gagcagctgg cctgggtctg gcgcagcaaa gcgcgctgta acccgttgtt
    21781 tatcgctacc ggccatcggg tcagcgtgga cagcgcgctg gcgtgggtac aacgctgcat
    21841 gaaaggctat cgtctgccgg agccaacgcg ctgggcggac gcggtggcct cggaacgtcc
    21901 ggcgttcgtg cgctatacag caaatcagcc ctaattcagg taaactgcgg ataatttccg
    21961 tatttgagaa ctcaacatgt tacaaaaccc aattcatctg cgtctggagc gcctggaaag
    22021 ctggcagcat gtcactttca tggcttgctt atgcgaacgc atgtacccca attacgccat
    22081 gttctgccag caaaccggtt ttggtgatgg gcaaatttac cgtcgtattc tcgatctcat
    22141 ctgggaaacg ctgaccgtta aagacgcaaa agtaaatttc gacagccaac tggagaaatt
    22201 tgaagaagcg attccttcag ccgacgattt cgatctgtac ggcgtttatc cggcaatcga
    22261 tgcctgcgtg gcgttaagtg aactggtcca ttcgcgtttg agtggtgaaa cgctcgaaca
    22321 cgcggtggaa gtgagtaaga cctccatcac gaccgttgcg atgctggaaa tgactcaggc
    22381 tggtcgcgaa atgagcgatg aagagcttaa agaaaaccca gctgtagagc aagaatggga
    22441 cattcagtgg gaaatattcc gacttttagc tgagtgcgaa gaacgcgata tcgagctgat
    22501 aaaaggcctt agggcagacc tgcgtgagtc gggtgagagc aatattggta taatttttca
    22561 gcaataagac cagaaaacgt gatttaacgc ctgatttgtc gtacctggag tcttcccttt
    22621 cgccccccgt ctggtctaca tttggggggc gaaaaaaagt ggctatcggt gcgtgtatgc
    22681 aggagagtgc tattctggca tttccgtcgc actcgatgct tagcaagcga taaacacatt
    22741 gtaaggataa cttatgaaca agactcaact gattgatgta attgcagaga aagcagaact
    22801 gtccaaaacc caggctaaag ctgctctgga gtccactctg gctgcaatta ctgagtctct
    22861 gaaagaaggc gatgctgtac aactggttgg tttcggtacc ttcaaagtga accatcgcgc
    22921 tgagcgtact ggccgcaacc cgcagaccgg taaagaaatc aaaattgccg cagctaacgt
    22981 accggcattt gtttctggca aggcactgaa agacgcagtt aagtaagatt gcgtggcagt
    23041 gaacagtttt aacgaagggg tggtttcacc ccttttgtct ttctggcgtc gatcattgat
    23101 gctggcaggt gcgctgtttc tcactgcctg tagccacaac tcttcacttc ctccctttac
    23161 cgccagtgga tttgctgaag accagggcgc ggtacgcatc tggcgaaaag acagcggcga
    23221 taatgtgcat ctgcttgccg tgtttagccc gtggcgcagt ggcgatacca cgacgcgaga
    23281 gtatcgctgg cagggcgata acctcacgct catcaatatc aatgtttaca gcaaaccgcc
    23341 ggtgaacatt cgcgcgcgtt ttgacgatcg cggcgatctg agctttatgc aacgtgaatc
    23401 cgatggggaa aagcagcagc tttctaacga ccaaatcgat ttataccgtt atcgtgctga
    23461 tcagatccgc cagattagcg atgccttacg tcaggggaga gtcgtgctgc gccaggggcg
    23521 ctggcatgcg atggaacaga ccgtgaccac ctgcgaaggg caaaccatta aacctgattt
    23581 agattcgcag gcgatagcgc atatcgagcg ccgccagagc cgctcttctg ttgatgtcag
    23641 cgtggcatgg ctggaagcgc ccgaaggttc gcaattactg ttagtggcaa actctgattt
    23701 ctgtcgctgg caacccaacg agaaaacgtt ctgatttacc agtggcccat acccatatga
    23761 ccgccaccac cgcagccgcc gtagcccatg cccattccgg ctccgcgcgg aatacccgct
    23821 tcagccatcg cgatatctcg tttcacccgt aactcatcta acgactgacg caaattctcc
    23881 atctctttgg cgaccgcgtt aattttgctg ctatccggtg ggttcgcggc taacagggca
    23941 ttgtattcat aacgcttcgt caccagttgc tgttgcagtg cgctgctttg agcgtaaaag
    24001 tcattatgga ttttctgcca cgctgtctgt tgttcgctgg tcaaaggcgc ggcattttgc
    24061 tgccacatac cgtgtccgcc gtgagcaaat gcagatgtcg atcccatcgc cattgctgaa
    24121 agcgccatca ttaccagggc aattttcgtg ttccgtttca tggttaatcc tccagtggtt
    24181 gtcttacttc gggtattgca tcttccgtgc caacgatgaa acgctgatat gacgggtaat
    24241 ctggcatgat aaacgagtaa aaatgactcg cctgctgcgg gtagcgagtc atttttactc
    24301 attgaaactt aagcctttgt gttacagcgc agggtaagcg ctgataaaag atggcatgat
    24361 ttctgctgtc agaaagggat gagcaggcaa agaagaagat gcgttttatg caacgttcta
    24421 aagactcctt agctaaatgg ttaagcgcga tcctccccgt ggtcattgtt gggctggtag
    24481 ggttgtttgc ggtgacggtg attcgcgatt atgggcgcga gactgccgcc gccagacaaa
    24541 cgctgctgga aaaaggcagt gtacttattc gcgctcttga atccggctcg cgcgtcggca
    24601 tggggatgcg catgcatcat gcgcagcagc aggccttact ggaagaaatg gccgggcagc
    24661 ctggagtacg ttggtttgcg gtcacggatg aacaaggaac aatcgtgatg catagcaact
    24721 ccggcatggt gggaaaacag ctttattccc ctcaggaaat gcagcagtta catccgggag
    24781 atgaagaagc gtggcggcgg atcgatagcg cagacggcga gcctgttctg gaaatttatc
    24841 gccagtttca accgatgttt gctgctggaa tgcaccggat gcgccatatg cagcagtatg
    24901 ccgcgacacc acaagcaatt ttcatcgctt tcgacgccag taatattgtg agtgccgaag
    24961 atcgtgagca gagaaacacc ctgattattc tcttcgccct ggcgacggtc ttgctggcaa
    25021 gcgtattgtc attcttctgg tatcgccgct atctgcgctc gcgccagctt ctacaagatg
    25081 aaatgaagcg caaagagaag ctggtggcgc tagggcatct tgcggcaggc gttgcccacg
    25141 aaatccgtaa cccactttcc tcgattaaag gactggcgaa atactttgcc gagcgcgcgc
    25201 ctgcaggggg agaagcgcat caactggcgc aggtgatggc gaaagaggcc gaccgtttaa
    25261 accgcgtggt aagcgagttg ctggaactgg ttaagccaac gcatctggct ttgcaggcgg
    25321 tggatctcaa cacgctgatt aaccactcat tacagctggt aagccaggat gcaaacagcc
    25381 gggagatcca gttacgcttt accgccaacg acacattacc ggaaattcag gccgatccgg
    25441 acaggctgac tcaggtcctg ttgaatctct atctcaatgc tattcaggcg attggtcagc
    25501 atggcgtgat tagcgtgacg gccagcgaaa gcggcgcggg cgtgaaaatc agcgttaccg
    25561 acagcggtaa gggaattgcg gcagatcagc ttgaagccat cttcactccg tacttcacca
    25621 ccaaagccga aggcaccgga ttggggctgg cggtcgtgca taatattgtt gaacaacacg
    25681 gtggtacaat tcaggtcgca agccaggagg gaaaaggctc aacgttcacc ctctggcttc
    25741 cggtcaatat tacgcgtaag gacccacaag gatgacgcac gataatatcg atattctggt
    25801 ggtggatgat gacattagcc actgcactat tttgcaggct ttactgcgcg gctggggcta
    25861 taacgtcgcg ctggcgaaca gcgggcgaca ggcgctggag caggtgcggg aacaggtttt
    25921 tgattttgtg ctttgcgatg tgcgaatggc ggagatggac ggcatcgcca cgctgaaaga
    25981 gatcaaagcg ttaaacccgg caattccggt gctgattatg actgcgtact ccagcgtcga
    26041 gacggcggta gaggcactga aaactggggc gctggattat ctcatcaagc cgctggattt
    26101 cgataaccta caggcgacgc tggaaaaggc gctcgcgcat acgcacagta ttgatgctga
    26161 aacacctgcg gtgactgcca gccagttcgg tatggtcggt aaaagcccgg cgatgcaaca
    26221 cctgctcagt gaaatcgccc tcgtcgcgcc atcggaagcc acggtgctga tccacggcga
    26281 ttccggcacc ggtaaagagc tggtcgccag ggcgattcac gccagtagcg cacgtagcga
    26341 aaaaccgctg gtaacgctca actgtgcggc actcaacgaa tccttgctgg aatctgaatt
    26401 gttcggtcac gaaaaagggg cgtttactgg agccgataaa cgccgggaag ggcgctttgt
    26461 tgaggcggac ggcggcacgc tgtttctcga tgaaattggc gatatctcgc cgatgatgca
    26521 ggtgcgtctg ctgcgtgcga ttcaggagcg cgaagttcag cgtgtcggta gcaaccagac
    26581 tatctcggtt gatgtccggc tgattgcggc gacccatcgc gatcttgccg cagaggtgaa
    26641 tgccgggcgt tttcgccagg atctctacta tcgcctgaat gtggtggcga ttgaagtacc
    26701 atcgctgcgg caacggcggg aagatatccc tctgctggct ggccattttc ttcagcgctt
    26761 tgccgagcgt aatcgcaagg cggtaaaagg ctttacgcca caggcgatgg atttgctgat
    26821 ccactacgac tggccgggaa atattcgtga actggaaaac gcggtggaac gggcggtggt
    26881 gctgctgacc ggggaatata tttccgaacg cgagctgccg ctggcgattg ccagtacgcc
    26941 gatcccgctg gggcaaagtc aggatattca gccgctggtg gaagtggaaa aagaggtgat
    27001 tctggcggcg ctggagaaaa cgggcggcaa caaaaccgaa gccgcccgtc agttagggat
    27061 cacgcgcaaa acgctattgg caaaactgtc gcgttagttc tgctcgcgtt cgatggcgcg
    27121 ccaaccgata tctttccggc agaagcaatc atcccagtgg atatcggtca ttaaggcgta
    27181 ggcgcgtttc tgcgcttctg ccacggtatg acccagcgcg gtgacgcaca gtacgcgccc
    27241 gccgctggtt accacctgct cgtcatccgc cagttttgtg cccgcgtgga acactttgcc
    27301 gtcagccact tcttccagcg gcaggccgtg gatcacatcg ccggtgcggt aatcgcccgg
    27361 atatccaccc gcagccatca ccacgctgag agaagcacgt tcatcccact ctgacgtttt
    27421 ctcgtccagt ttgccttcac aggccgccag gcagagctca accagatcgg acttcatgcg
    27481 cagcataatc ggctgggttt ccggatcgcc aaagcggcag ttaaattcaa taaccttcgg
    27541 attgccctgt ttgtcgatca tcaggcccgc atagagaaaa ccggtgtagg tgttgccttc
    27601 cgccgccatg cctttcacgg ttggccagat gatacgttcc atggtgcgct gatgaacttc
    27661 atcggttact accggcgcgg gggagtaagc gcccatcccg ccggtgtttg gtccgttatc
    27721 tttatcgcct acgcgtttgt gatcctggct ggtagccatc ggcagcacat gctcgccgtc
    27781 caccatcacg ataaagctcg cttcttcgcc atcgaggaac tcttcgataa cgatgcgatg
    27841 acccgcgtcg ccaaaagcgt tgcccgccag catatcgtga acagccgctt ccgcttcttc
    27901 cagcgtcatc gccacgataa cgcctttccc ggcagccaga ccgtccgctt taatgacgat
    27961 tggcgcgcct ttctcacgca gatacaccag cgcaggttct acctcggtga agttctggta
    28021 ttccgccgta gggatcttat ggcgggccag gaaatctttg gtaaacgctt ttgagccttc
    28081 cagttgggcc gcacctgcgg ttgggccgaa gattttcagc ccggcggcgc ggaaggtatc
    28141 gaccacgcct ttcaccagcg gcgcttccgg gccgacgatg gtcagatcaa tcttttcgtt
    28201 ttgtgcgaaa tccaacagcg ccgggatatc ggtcacgccg atagcaacgt tttgcagcgc
    28261 gggttccagc gcagtgcctg cattacccgg agcaacaaaa acagtctcaa ccagcggcga
    28321 ctgggccgct ttccaggcca gcgcgtgctc gcgcccgccg ttaccaatca ctaatacttt
    28381 catctattgc tccattaatg gcggaagtgg cgcatgtcgg tgaagagcat cgcaataccg
    28441 tgctcgtcgg cggcggcaat cacttcgtca tcacggatgg aaccgccagg ctggattacg
    28501 caggtcacgc ccgcagcggc ggcggcatca ataccgtcgc ggaacgggaa gaacgcgtca
    28561 gaagccatcg aggaaccttt cacttccagg ccttcatcgg cagctttaat accggcgatt
    28621 ttcgcggagt acacgcggct catctggccc gcgccaatac cgatggtcat attgtttttg
    28681 gcatagacga tagcattgga tttcacgaat ttcgccactt tccagcagaa cagtgcatca
    28741 cgcagttcct gttcggtcgg ctgacgttgg gtgacgacgc gcagttcttc cgcaccgacc
    28801 atccccaggt cacgatcctg aaccagcaga ccgccgttca cgcgtttgaa atcaagaccc
    28861 ggaacacgct cgccccactg accgcaggtc agaacgcgta cgttctgctt ggcggcggtg
    28921 attttcaggg cttcttcgct ggcggaaggc gcaataatca cttcgacaaa ctgacgagaa
    28981 atgatggcct gtgcggtttc cgcatccagc tcgcggttaa aggcaatgat gccgccgaat
    29041 gcggaggttg ggtcggtttt gtacgcgcga tcgtaagcat caagaatgga attgccgata
    29101 gctacaccgc aagggttggc gtgcttcaca atcacacatg ccggctcggc gaactctttc
    29161 acgcactcca gcgccgcatc ggtatcggcg atgttgttat aagagagggc tttaccctga
    29221 acctgggttg cggtagcaac ggaggcttct ttcacattct cttctatata gaaggcagcc
    29281 tgctggtggc tgttctcgcc gtaacgcata tcctgcttct taatgaagtt caggttcagc
    29341 gtgcgtggga agcgaccggc ggcttctttg ctttcaccgt ggtacgccgg aaccatgctg
    29401 ccgaagtagt tggcaatcat gctgtcgtag gcggcagtgt gttcgaaggc tttgatggcg
    29461 aggtcgaaac gggttgcaag cgtcagcgat ccttcgttgt catccatctc tttaataatg
    29521 gcgtcgtagt cgctgctctt caccacgatt gcgacatctt tatggttctt agcggcggag
    29581 cgcaccatgg tcgggccgcc gatatcgatg ttctcaaccg catcttccag cgagcagcct
    29641 tcacgagcaa cagtctgggc gaacggatac aggttaacaa ccaccatatc gataggctgg
    29701 atctgatgtt cttccataat ggcatcgtcc tggccgcgac ggcccagaat gccaccatgt
    29761 actttcggat gcagggtctt cacgcgtcca tccatcatct ccgggaaacc ggtgtaatcg
    29821 gaaacttcgg ttaccggcag acctttttct gctaacagac gggcagtgcc ccctgtagac
    29881 agcagctcca caccgcgtgc ggaaagtgcc tgggcgaatt cgacgatacc ggctttgtca
    29941 gaaacactga gcagagcgcg gcggactgga cgacgttgtt gcatggtaaa tcccctgggt
    30001 ttgactatta cagagagcgt tagctgaatt tttcgcgaaa aactcagcta acgcccctaa
    30061 cggggcatcc ttatttttcg cccgcattgt aacgaaaacg tttgcgcaac gctcgcgaat
    30121 ttttctcttt caatggtgat cacaattttg acagtggtta ccgtgggaaa aatacagaaa
    30181 ttacattgat gattgtggat aactctgtgc gtaaaaaggt ataaagcggg gttttgctgg
    30241 ggaatgcagc agtcagtcat ttttctgcaa tttttctatt gcggcctgcg gagaactccc
    30301 tataatgcgc cttcatcgac acggcggatg tgaatcactt cacacaaaca gccggttc
//
LOCUS       DAJTYG010000043        29797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTYG010000043 DAJTYG010000000
VERSION     DAJTYG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.043"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..218)
                     /locus_tag="OO568_002063"
     CDS             complement(<1..218)
                     /locus_tag="OO568_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6641419.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAEVY"
     gene            complement(399..1874)
                     /locus_tag="OO568_002064"
     CDS             complement(399..1874)
                     /locus_tag="OO568_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6641420.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLALLSGLIYLVETIVVIHKPISDSTLIRTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            complement(2021..3304)
                     /locus_tag="OO568_002065"
     CDS             complement(2021..3304)
                     /locus_tag="OO568_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCR6641421.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSSESVSIPTEDISEPMFHQGRGGLRHRVHTGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIEHVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(3417..5351)
                     /gene="yeaG"
                     /locus_tag="OO568_002066"
     CDS             complement(3417..5351)
                     /gene="yeaG"
                     /locus_tag="OO568_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6641422.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEDNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5787..6533
                     /gene="mipA"
                     /locus_tag="OO568_002067"
     CDS             5787..6533
                     /gene="mipA"
                     /locus_tag="OO568_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6641423.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            6623..7384
                     /locus_tag="OO568_002068"
     CDS             6623..7384
                     /locus_tag="OO568_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6641424.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLCYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLVQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(7435..8319)
                     /locus_tag="OO568_002069"
     CDS             complement(7435..8319)
                     /locus_tag="OO568_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6641425.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGSVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(8403..9398)
                     /gene="gapA"
                     /locus_tag="OO568_002070"
     CDS             complement(8403..9398)
                     /gene="gapA"
                     /locus_tag="OO568_002070"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6641426.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9740..10153
                     /gene="msrB"
                     /locus_tag="OO568_002071"
     CDS             9740..10153
                     /gene="msrB"
                     /locus_tag="OO568_002071"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6641427.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            10195..10467
                     /locus_tag="OO568_002072"
     CDS             10195..10467
                     /locus_tag="OO568_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6641428.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            10837..11913
                     /locus_tag="OO568_002073"
     CDS             10837..11913
                     /locus_tag="OO568_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6641429.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            11940..13319
                     /locus_tag="OO568_002074"
     CDS             11940..13319
                     /locus_tag="OO568_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6641430.1"
                     /translation="MEQITKPHCGVRLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESCGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYLLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAASIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            13336..14381
                     /locus_tag="OO568_002075"
                     /pseudo
     CDS             13336..14381
                     /locus_tag="OO568_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            14402..15238
                     /locus_tag="OO568_002076"
     CDS             14402..15238
                     /locus_tag="OO568_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR6641431.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            15243..16191
                     /locus_tag="OO568_002077"
                     /pseudo
     CDS             15243..16191
                     /locus_tag="OO568_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            16201..17181
                     /gene="ydjG"
                     /locus_tag="OO568_002078"
     CDS             16201..17181
                     /gene="ydjG"
                     /locus_tag="OO568_002078"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR6641432.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDPALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            17318..18076
                     /locus_tag="OO568_002079"
     CDS             17318..18076
                     /locus_tag="OO568_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6641433.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            18194..19552
                     /locus_tag="OO568_002080"
     CDS             18194..19552
                     /locus_tag="OO568_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6641434.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFLRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(19645..20286)
                     /gene="pncA"
                     /locus_tag="OO568_002081"
     CDS             complement(19645..20286)
                     /gene="pncA"
                     /locus_tag="OO568_002081"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6641435.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(20297..21313)
                     /gene="ansA"
                     /locus_tag="OO568_002082"
     CDS             complement(20297..21313)
                     /gene="ansA"
                     /locus_tag="OO568_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6641436.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRL
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNVLAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(21480..23336)
                     /gene="sppA"
                     /locus_tag="OO568_002083"
     CDS             complement(21480..23336)
                     /gene="sppA"
                     /locus_tag="OO568_002083"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6641437.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23497..24048
                     /locus_tag="OO568_002084"
     CDS             23497..24048
                     /locus_tag="OO568_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6641438.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            24165..25208
                     /gene="selD"
                     /locus_tag="OO568_002085"
     CDS             24165..25208
                     /gene="selD"
                     /locus_tag="OO568_002085"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6641439.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            25213..27174
                     /gene="topB"
                     /locus_tag="OO568_002086"
     CDS             25213..27174
                     /gene="topB"
                     /locus_tag="OO568_002086"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6641440.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIEQDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSVAIA"
     gene            27301..28341
                     /locus_tag="OO568_002087"
     CDS             27301..28341
                     /locus_tag="OO568_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6641441.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIAVLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(28458..>29797)
                     /gene="gdhA"
                     /locus_tag="OO568_002088"
     CDS             complement(28458..>29797)
                     /gene="gdhA"
                     /locus_tag="OO568_002088"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6641442.1"
                     /translation="QTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYR
                     QMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVNLS
                     ILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGADT
                     DVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTE
                     AMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARAITASDSSGTVVDESGFTKEK
                     LARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDTAHQLI
                     ANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWK
                     AEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
BASE COUNT         7667 a         6861 c         7570 g         7699 t
ORIGIN      
        1 cagtacacct cagcgagagc gcgcatcaca tgcgcggcaa tactctgatt ctgatactta
       61 tcataaagaa aggaggagtc tgctttctgc acgatatagc gcagataggc actcattgct
      121 ctgtcgctgg cgacaagttc aaaaataagg aaggaagatg tcagcacaat gacacttgcg
      181 gcgataaaat gccggagcat tctatggtgc aatttcatga taaatggcac tccttcggtt
      241 agtgaaacga gaaaaccaga taaagttcga ttgcggcctg gcacaggggc gctaattata
      301 cttatctctg cgccgctggg gaggggaaat ttaggggtaa tagtaataaa ttgtatgcaa
      361 aagccacgca aaaagcatgg ctctgaatat ttttgcactt aaatattatg aataatcact
      421 ttgttgcccc cctcgctttt cgcttcgcgt aaggctttat ctgcttccgt catgacaagc
      481 gagatattac gagtgtcacc cgttgaaaaa aacgtgccaa tactgatggt catttgttca
      541 ggaacatcat atctgttact gaagccggtc aggagttcga catttttacg catacgttcc
      601 gcaatgattt ttgcctgggc actattgagt tcggtaaata gcaagccaaa cacctcaccc
      661 tccagtcgcg ctaaaatatc atcggggcgg atgctttgct ggataatatc gacaattgaa
      721 accagcactt tatcacccac acgatgtccc cattgggcat tgatgcgttt gaactgatta
      781 atatcaacca gcatgacaca gaagtcctta acaacaggtc gcgacaataa tgactctacg
      841 ctgttgaaaa agtagcgccg attataaata ttggtcagca catcatgaac tgccagtttg
      901 ctggagagtt gcagttcctg aaaaatgtta taaatcagaa aagacacaac aaacagttta
      961 ctggagactt caatcccgcg actgatgtac cagatggtgt aattatactc atccagagtc
     1021 agtagaagta agttgcagca tagcgatgcc agacataata atgctattaa tggccatagt
     1081 tcgctggcta atcgtgtacg catgataata aagaacagta aaaatgccca cagacaaacc
     1141 aatatttttg tatagttgat tccccaggtc gcagtatggt tgtcaggaca gtaatcgacg
     1201 acatacaaag aatagtcagc gttataactg ctcagattgt gtgccagaag gggaaaaact
     1261 aaaaaaggaa tcagcgccag caacagaatg ccagtttttt tcttattgtt gtcaaggatg
     1321 ttgtctttac cataacaaaa gagcgccaac gaggttaaac aaataaaact gagttggcgg
     1381 aaaatataga aaatagaaac atcattcgac tttgtccgga tcagtgtact gtcgctgata
     1441 ggtttatgga taacaacaat agtttcaact aaatagataa ggccacttaa aagtgcaaga
     1501 cttaaaatga gtgttggtag aatttgtttg ttagtgaaat atttcattaa catgaaaaga
     1561 aaaatgatgg cgttaaggaa aaacaaagcc acaacgacca tcagataatt catttgtggg
     1621 atgagtttta cattcgtctt ttcgttatat aatgtggtga tgcaaaataa aattaacgtc
     1681 agcaataaac tgaaaataaa aaagctttga gcttttaatt tttgtgtgcc catagaaacc
     1741 ataaaaagga gagcaatgtg agtgctggat tttattgatg gcagagatat atagttttga
     1801 ccaagatcaa ttttcgttaa cgataaaaaa tatttaaaga ataaccccag ggaaatacgt
     1861 gttgactgaa tcatttttcc gaatgattgt tttctggaac taaattgtgt aaaggttatg
     1921 ctgtcatatt taaagcgatt gtaagctaat gtatgtaata aatgagataa tttacagtaa
     1981 gctaaaaatc agccaggcga taatgacccg gctgatagtt ttagtcttta gctgttgcat
     2041 tttgtttatg aaacagttca cggaacaccg gataaatatc atcctggtcg cggatgtgct
     2101 gcatcgcaaa gttgtcgaaa gtagattgca gatgctcata ttctcgccac aatgtctgat
     2161 gtgcacgacg ggtaatttcg atatagctgt aataacgaac aacaggtaat aattttttcg
     2221 ccaggatttc atggcaaagc ggagagtcat cggcccagtt atcgccgtcc gatgcttgtg
     2281 cagcgtaaat attccactgt gccgggttat aacgctcttt cactacctca tccatcagtt
     2341 tcagggcgct ggaaacaatg gtgccgcctg tttcctgcga gtagaaaaac tcatgttcat
     2401 cgacttcttt cgcctgggta tgatggcgga tgtatacgac ttccacgttc ttatacgttc
     2461 tgctgaggaa cagatacagc agaatataaa aacgcttagc catatcttta gtggattgat
     2521 ccattgaacc ggaaacgtcc atcaggcaaa acatcactgc ctggctggag ggatcgggcc
     2581 gcttctcgta gttcttgtaa cgtaaatcga aggtgtcaat aaaagggacg tgttcaattt
     2641 tggcacgtaa ttctgcaatt tctttgcgca gacgttcctc ttccagcagc tgcgcaggtt
     2701 cactgttgct gatgatggcc aaattctctt ccagtgcatg aagttcccgc cgcttgcctg
     2761 ccgtcatggc tgtgcgtcgc gccagtgagt tctgcaagga acgcacaacg ctgatattgg
     2821 ctggaacgcc gttagcggta taacccgccc gatgcgtttt atattcggtc agctggcgtt
     2881 gttggttttg tttcagattc ggtaaggcca aatcttcaaa gagcagatca agatactcat
     2941 ctttcgaaat ctgaaagaca aattcatcct gaccttcacc atcctggctg gcctggccct
     3001 gaccactgcc ggaacctccg ccgccaccct ggggacgttc aattcggtcg ttctgaacga
     3061 aatggtcatt gcccgtatgc acgcggtggc gcagaccgcc acgcccctga tgaaacattg
     3121 gttcgctaat atcttccgtg ggaatggata cggattcgct gctgtcgacg tcagtcaccg
     3181 aacgcttatt aatggcctcg gagatcgact gtttaatttg cgctttataa cggcgtaaaa
     3241 aacgctggcg attcaccatg cttttgtttt tgccgttcag acgccggtca ataaaccagg
     3301 tcatatgccc cccgtactac atttgccaac tgtatgctac gcctgtgttg ccgcttctca
     3361 ggcccacggt tcgctatcgt tgtaggcccg gtagcatgtg taccgggcca gggttgttaa
     3421 gacgatttac gtacgcgcaa ataccattcg cacagtaaac gcacctgttt acgggtgtag
     3481 cctttttcca tcatacggtc gacaaagtcg tcgtgtttct tctgctcgtc ggttgacgtt
     3541 ttggcgttaa acgagataac cggcaacagc tcctcggtat tggagaacat tttcttctcg
     3601 ataaccgtgc gcagtttttc atagctggtc cagttcggat tgcgtccgct gttattcgct
     3661 ctggcgcgca gtacgaagtt aacaatctcg ttgcggaaat cttttggatt actgatcccc
     3721 gccggtttct cgattttctc cagctcggcg ttaagagact cgcggtcaaa cagctgcccg
     3781 gtatccggat cgcgatactc ctgatcctga atccagaaat ccgcgtaggt aacataacgg
     3841 tcgaaaatgt tttgcccata ttcggaatag gattcaaggt aggccgtctg gatctctttg
     3901 ccgataaact cggcatattt cgggatcaga taacctttca ggaactccag atagcgttct
     3961 gcctgctctt gcgggaactg ctcgcgctcg atctgctgtt ccaggacgta gaacagatgg
     4021 accgggtttg ccgccacttc tacatgatcg aagttgaaca cgcgggagag gatcttaaac
     4081 gcaaaacgcg tcgacagacc gttcatccct tcatcgacac cggcgtagtc acgatattcc
     4141 tgatacgact tggctttcgg atcggtgtct ttcagacttt cgccatcata aacccgcatc
     4201 tttgaataaa tgctggagtt ttctggctct ttcaggcgcg aaagaatgga aaaacgtgac
     4261 agtgtttcga gcgtgccagg ggcgcatggg gcgtgagtca attcactgtg attaagcaat
     4321 ttctcgtaga ttttgatctc ttcggaaatg cgcaagcaat acggcacctt cacgatgtaa
     4381 acacggtcga ggaaggcttc gttgtttttg ttattacgga aagtgaccca ttcggactcg
     4441 ttcgagtggg cgagaataat cccgttgaac ggcagggcgg agataccttc cgtcccgttg
     4501 tagttatctt cctgagtggc ggttaacaag ggatgcagca cttttatcgg tgctttaaac
     4561 atctcaacga attccatgat cccctgattg gcgcggcaca gcgcaccgga atagccgtag
     4621 gcgtccgggt cattctgcgc gtagtgttcg agtttacgaa tatcgacttt cccaaccagc
     4681 gcggagatgt cctggttgtt ctcatcaccg ggttccgttt tggcgatagc aatttgttgc
     4741 agaattgacg gccagacctt cactacccgg aacttagtga tatcgccacc aaattcatgc
     4801 aggcgttttg ccgcccatgg cgacatgatg gtgccgagat aacggcgagg aatgccatac
     4861 tctttttcca gaatctgagc atcttcctgc ggattgaaaa gacagaacgg atgatcgttg
     4921 accgggctac gctcaccgtt ggcgctcaat acataaatcg gtacgagctg cattaatgat
     4981 ttcagtcgct cagcaagcga tgatttaccc ccacccacag gccccagcag atacaggatt
     5041 tgtttcttct cttccagccc ctgagccgcg tgtttcagat aagagacaat ctgttcaatc
     5101 gcgtcttcca tgccgtaaaa ctcttcaaat gccggataac gtgcaatgac ccggttagaa
     5161 aagagtcgag aaagtctggg ttcctgggct gtatcgacca tgacaggctc accgatagcc
     5221 atcaatagcc gctcagccgc gttggcataa gcactgcgat cttgccgaca agtggtaaga
     5281 aactcctgca gtgtgaactc ttcgtccttg gcagcttcat atcgctggcg atagtgatcg
     5341 aatatattca tggtatgccg tcctttcgtt ttttagcaca ggttaagagc cgttcatatg
     5401 aatagtagag gctcccggaa gaggtaaact gaacgacgtc tcttgctaaa acacagcaac
     5461 tctcatgcca ttttatcgac gatgagaaca acgtgcccgg tgatacacct tcaaaattaa
     5521 gcgtagatgg caaatggaaa acttgcctgc acggctttct taatttcaat ggcatatcaa
     5581 caactcatcc ataaattttg cataattaat gtaaagacca ggctcgccag taacgctaaa
     5641 tccatttggc tgtaagcgcg gtgtcatccg cggcaggaaa attaaacagt tactttaaaa
     5701 aatgaaaacg taaaaaggtt gggtttcgat gtattgacgg gtaaactttg tcgcccgcta
     5761 aacatttgtt taaggaatga ttaattgtga ccaaactcaa acttctggca cttggagtgc
     5821 ttatcgcaac gtctgcaggc gtagcgcacg ctgaaggtaa attttccctg ggcgcaggcg
     5881 taggtgtcgt tgagcaccca tataaagatt acgataccga tgtttaccca gtaccggtaa
     5941 tcaactatga aggcgataac ttctggttcc gtggcttagg tggtggttac tacctgtgga
     6001 atgacgcaac ggataaactt tcaattaccg cttactggtc gccgctttac ttcaaagcga
     6061 aagacagtgg cgatcaccaa atgcgtcacc tggatgaccg taagagcacc atgatggctg
     6121 gtctgtctta tgctcacttt acccagtacg gttacctgcg taccaccctg gctggcgata
     6181 ccctggataa cagcaacggc attgtctggg atatggcttg gttgtatcgt tacaccaacg
     6241 gtggcctgac cgtgactccg ggtattggtg tgcagtggaa cagcgaaaac cagaacgaat
     6301 actattatgg cgtatcgcgc aaagagtccg ctcgcagcgg tctgcgtggg tataacccga
     6361 atgacagctg gagcccttac cttgagctga gcgccagcta caacttcctc ggcgactgga
     6421 gtgtttacgg taccgcgcgc tacacccgtc tgtctgatga agttactgac agcccgatgg
     6481 tggataaatc ctggactggc ctgatttcta ccgggatcac ctacaaattc tgataatgca
     6541 tctttccagg gcattttgtg cataaaaata gggcgtcacg ccctgttttg cattacggtg
     6601 gtgcaatacc tgggaggaaa caatgcaaca aaaaatgatt caatttagtg gcgatgtctc
     6661 actgccagcc atagggcagg gaacatggta tatgggcgaa gatgccagtc agcgcaaaac
     6721 agaagttgct gcactacgcg caggcattga actcggttta accctcattg ataccgccga
     6781 aatgtatgcc gatggcggtg ccgaaaaggt ggttggggaa gcattaaccg gtctgcgaga
     6841 gaaggtcttt ctcgtctcta aagtctatcc gtggaatgct ggcgggcaaa aagcgataaa
     6901 tgcatgcgaa gccagtttac gccgtctcaa tactgattat ctcgatctct acttattaca
     6961 ctggtctggc agtttcgctt ttgaagagac tgttgctgcg atggaaaaat tgatcgccca
     7021 gggaaaaatc cgtcgctggg gcgtttctaa cctttgttat gccgatatgc aggaactctg
     7081 gcagctgccg gggggaaatc agtgtgccac taatcaggtg ctttaccatc tcggttcacg
     7141 agggattgag tacgatctac tcccctggtg ccagcaacag cagatgccgg tgatggctta
     7201 cagtccatta gtccaggccg ggcggttgcg caatggactg ttaaaaaacg cggtagtcaa
     7261 cgaaattgca catgtccaac aaaatgcagc tgcgcttgag gtcgaacttt cgtcagcgga
     7321 attagctatg ctggataagg catatccggc accaaaagga aaaactgcgc tggatatggt
     7381 gtgatgttga tacgtgccgg atgcatcgca cccggcacac aacattaacg caaattaacg
     7441 tttggcaacg cgaatggatt gcgccagatg tgcaggtttc tctttggtga ctttttgcgt
     7501 ttctgaagcg taagccgttt ctacacaaac aaaggtcttg tagccatcgt ccggcatgtc
     7561 gcccatgcta actgaaagcg ccggtcccgg gttccagccg acaacgttca gatgatgctg
     7621 gtggcctacg gcgataatac gattcagcgc ttcatcatta atcacgctgc aatcttgcgg
     7681 attcagatac acgcgatcgg tacggtcagg gaaggtctga ataccgtcgg tcagtacatc
     7741 ttctttcgcg tcattcactt tatcaatgaa gcgatcgccc agcccactga cgcttacctt
     7801 agcgatatca cccacgttaa agtaggtatg cagggcagag gtggtttcaa attcgccatg
     7861 tgattcaaga tcgatttcac aggttttacc cacgcggaaa tgcgctaaca gcgtaaagtc
     7921 gtgcggccag aattttttcg tctcttcgct ttgtgtcaat tcaaaagtca gcgctacgcc
     7981 atcagcatct tcatgatgtg atttcagcgt ccacggcagg ttgcgggcaa aaccgtgcgc
     8041 aggcagacct tgttgtgccg ccggaccaaa ccacggccag caaaccggta cgctaccgcg
     8101 aatagcgacg ccatttttga acggtgtgtt gttgctcaac cacagaactt cttcttcacc
     8161 cgcaggtttc cacgagagaa ggtgtgcgcc ctgtaatgca aaagaggctt ttacctgggg
     8221 atgatcgacc acaatgaggt ccagttcatc cagtttacga cgggagagga caggggagat
     8281 ttgttcgatg accggaaggg caaaaatttt cttaatcatg acgcagtcct ttaacttcat
     8341 tttatcaggt aaaaaaaaga gcgaccgaag tcgctctttt tagatcacag tgtcatctca
     8401 acttatttgg agatgtgagc gatcaggtcc agaactttgt tggagtaacc ggtttcgttg
     8461 tcgtaccaag ataccagttt cacgaagttg tcgttcagag cgataccagc tttagcatcg
     8521 aacacggaag tgcaaacttc gccgttgaaa tcggtagata ctacgtcatc ttcggtgtag
     8581 cccagaacgc ctttcatttc gccttcagca gcagctttaa cggcagcttt gatctgctcg
     8641 taagttgcag ctttttccag acgaacggtc aggtcaacta cggatacgtt cggggtcgga
     8701 acgcggaacg ccataccagt cagtttgcca ttcagttctg gcagtacttt acctacagct
     8761 ttagcagcac cggtagagga cgggatgatg ttctgggaag cgccgcggcc gccgcgccag
     8821 tctttgtgag acgggccatc aacggttttc tgagtagcgg tagtagcgtg aacggtggtc
     8881 atcagacctt cgatgatgcc gaagttatcg ttgataactt tagccagcgg agccaggcag
     8941 ttggtggtgc aggaagcgtt ggaaacgatg tcctggccag catatttgtc gaagttagcg
     9001 cctttaacga acatcggagt gttgtcttta gacggaccag tcataaccac tttcttcgca
     9061 ccagcggtga tgtgtttacg agcagtttcg tcagtcagga acagaccagt tgcttcagcg
     9121 acaacgtcaa cacccacttc gtcccatttc aggttagccg gatcacgttc agcggtaaca
     9181 cggatttttt taccgttaac gatcagatga ccgtctttca cttcaacggt accgtcgaaa
     9241 cggccgtgag tggagtcata tttcagcatg tatgccatgt aatcagcgtc taacaggtcg
     9301 ttgattgcaa cgatctcgat gtcagaacgt ttctgagcag cacggaaaac aatgcgaccg
     9361 atacggccaa aaccgttgat acctactttg atagtcatat attccaccag ctatttgtta
     9421 gtgaataaaa ggttgcctgt aaaattacaa aaaccttacg cagcgtcaag cggaatcgtg
     9481 tcaatcattg cgacaaatca atcctgtgcc taagcattac gcggctgact cgcctcactc
     9541 ttcctttggg cttgagacca catggggtcg gcgccccgaa ttttaaaggg caattacgat
     9601 aaaaatgtga ttttcatcac gatttaggtg cagccatttc gtcgagatca agtttaggac
     9661 aaaattttgc cagttggtgt taatgttttg ttagaatcag tcacgtaatg tgagcacgat
     9721 taaagtgaga tgtgagtaaa tggcgaataa accttcggca gaagaactga aaaaaaattt
     9781 gtccgagatg cagttttacg tgacgcagaa tcatgggaca gaaccgccat ttacgggtcg
     9841 tttactgcat aacaagcgtg acggcgtata tcactgtttg atctgcgatg ccccgctgtt
     9901 tcattccgaa accaagtatg attccggctg tggctggccc agtttctacg aaccggtgag
     9961 tgaagaatcc attcgttata tcaaagactt gtcacatgga atgcagcgca tagaaattcg
    10021 ttgcggtaac tgtgatgccc atctggggca tgtctttccc gacgggccgc aaccaacagg
    10081 cgaacgttat tgtgttaact ctgcttcttt acgcttcact gatggcgaaa acggcgaaga
    10141 aatcaacggt tgaaaaaacg attcagcaac ttattccaca ggagcggaaa aattatgaat
    10201 cttgatgaca ttatcaacag catgacgcct gaggtatacc agcgtttgtc gaccgccgtt
    10261 gaactgggga aatggcctga tggcgttgcg ttgaccgagg aacaaaaaga gaattgcctg
    10321 caactggtga tgctgtggca agcccgccat aataccgaag cacagcatat gacaattgac
    10381 acaaatggtc agatggtcat gaagagcaaa cagcagttaa aagaagattt tggtatctca
    10441 gcaaagccta ttgccatgtt taagtaagtg gttcatcccc tttttgccgc ggctatgaag
    10501 gggatagcca acaatgaccc cacgactcgt ctcaattatc tcgacccgac ctggctaagt
    10561 cccagtccga tctcttgcag tcgcgttcac gcctgaatga cttctcctga acttaagtct
    10621 cagacctatt tggccggtaa tccctctcga atttgctctt tttgcgtccc gccttccacc
    10681 aacctgtaac agacccagcc attttctgcg taattgtgat ctaagttgta aaatgcaggt
    10741 cattatctgt gttgtgcaga tttaatgttg acctcaaaaa tcatgtgggt aagtatgatg
    10801 gccatctaag caaaacaaaa cacagaggat gacgcaatga aagcactggc tcggtttggc
    10861 aaggcctttg gcggctacaa gatgattcat gtcccacaac ccatttgtgg cccggaagac
    10921 gtagtgattg aaattaaagc cgcggcaatc tgcggcgcag acatgaagca ctacaatgtc
    10981 gatagcggtt ctgatgagtt taactctatc cgcggccatg agttcgcagg ttgtattgcg
    11041 caggttggtg aaaaagtcaa agactggaaa gtggggcaac gcgtcgtgtc ggataacagc
    11101 ggtcacgttt gcggcgtttg tccggcctgt gaacagggtg attttctgtg ttgtacagaa
    11161 aaagtaaacc ttggtctgga taacaatacc tggggcggtg gtttttccaa atattgtctg
    11221 gttcctggtg aaattctcaa aattcatcgt catgcgttgt gggaaatccc tgatggtgtt
    11281 gattatgagg acgcagccgt acttgaccct atctgtaatg cctataaatc catcgcgcag
    11341 caatcgaaat tccttcctgg tcaggatgtt gtcgtcattg gcactggccc actcgggctg
    11401 ttttccgtac aaatggcgcg gattatgggg gcggtaaata tcgtcgtcgt tggtctgcaa
    11461 gaagatgtgg cggtccgctt cccggttgca aaagaactgg gggcgacggc agtagtaaat
    11521 ggttctaccg aagatgtggt ggcgcgctgc cagcaaattt gtggcaaaga taatctggga
    11581 ctggtgattg aatgctccgg tgccaatatc gcactgaaac aagccatcga aatgctccgc
    11641 ccgaatgggg aagtggtacg cgttggaatg ggcttcaaac ctcttgattt ctcgattaat
    11701 gacattaccg cctggaataa aagcatcatt gggcatatgg cctatgactc cacctcatgg
    11761 cgtaacgcta tccggctatt agccagcggc gctatcaaag tcaaaccgat gatcacgcat
    11821 cgtatcggcc tgtcgcaatg gcgcgaaggg tttgatgcga tggtcgataa aaccgcaatc
    11881 aaagtgatca tgacttacga ctttgatgaa taactactct cctgaaggaa cgttatcgca
    11941 tggaacagat aacaaaaccg cattgtggtg tacggctgga tcgcttaccg gattgccgct
    12001 ggcattcgtc aatgtttgcc atcgtcgcgt ttggcttgct tgtctgctgg agtaatgccg
    12061 ttggtggctt gatcctcgcg cagctgaaag cgttgggctg gacagataat tccaccacag
    12121 ccacattctc agcaatcacg accgccggaa tgtttctcgg tgcgctggtg ggcggcatca
    12181 ttggcgacaa aaccggtcgc agaaatgcgt tcatcctcta tgaggccatt catattgcct
    12241 cgatggttgt cggtgctttc tcaccgaata tggattttct tattgcctgc cgttttgtga
    12301 tggacgttgg gctgggagct ttactggtta cactgtttgc tggtttcacc gaatatatgc
    12361 ccggtagaaa tcgcggaacg tggtccagtc gggtttcctt tattggcaac tggtcatatc
    12421 cgctctgctc actgattgcg atgggactca cgccgctgat tagtgcagag tggaactggc
    12481 gggtacaact gcttattcct gcaatattgt cgcttatcgc tacggcgctg gcctggcgct
    12541 actttcctga atccccgcgc tggctggaat cgtgcggacg gtatcaggaa gccgagaaag
    12601 tcatgcggag tatagaagaa ggcgtcatac gccagacggg taaacctttg ccgcctgtgg
    12661 ttattgctga tgacggtaaa gcgccacaag cggtgccgta ttcagcctta ctgacaggag
    12721 tattactgaa acgcgtgata ttaggctctt gtgtgctgat taccatgaac gttgtgcagt
    12781 acacactaat taactggttg ccaacaatat tcatgaccca ggggattaat ttaaaagact
    12841 cgattgtttt aaataccatg agtatgtttg gcgcgccatt tggtattttt attgccatgc
    12901 tggtgatgga taaaattccg cgtaaaacaa tgggtgtggg gctattaatc ctgattgcgg
    12961 tgctcggata tatctattta ctgcaaacca gtatgttgct cataacgctg attggtttct
    13021 tcctgattac tttcgtctat atgtacgttt gctatgcctc ggcagtgtat gtccctgaaa
    13081 tctggccgac agaggccaaa ctccgtggct ccggtctggc gaatgcggta gggcgaatca
    13141 gtggtattgc cgcaccttat gccgttgcag tgctgctcag tagttatggt gtaacgggag
    13201 tctttattct tctgggggct gcttcaatta ttgtcgcaat tgctatcgcc accattggaa
    13261 ttgaaaccaa aggtgtctcc gttgaaagtt taagtattga tgcagtagcc aataaataat
    13321 acgggtggat tcaaaatgaa aaattcaaaa gcaatattgc aggtgccggg cacaatgaaa
    13381 attatttcag cagaaatacc agtgcctaaa gaagatgaag ttttgattaa agtagaatat
    13441 gtcggtattt gtggttcaga tgtacatggt tttgaatcag gcccgtttat tccgcctaaa
    13501 gacccaaatc aagaaattgg cctgggtcat gaatgcgccg ggacggttgt ggctgtggga
    13561 agccgcgtgc gcaaatttaa accgggggat cgggtaaata tcgaacctgg cgttccttgc
    13621 ggtcactgtc gttactgtct ggaaggcaaa tataacatct gcccggacgt tgattttatg
    13681 gcgacacaac ccaactaccg cggcgcatta acgcactatc tgtgtcatcc ggagagcttt
    13741 acttacaaac tgcccgacaa tatggacacg atggaagggg cgctggtgga gcctgccgca
    13801 gtcgggatgc atgccgcgat gctggcagat gttaaaccgg gtaagaagat aattattctg
    13861 ggagcaggtt gtattggttt gatgacgttg caagcgtgca aatgcctggg cgcaacggaa
    13921 attgccgtcg ttgatgtact ggaaaaacgt ctggcaatgg gggaacagct tggtgcgaca
    13981 gtggttatta atggcgcaaa agaggacact attgcacgct gtcagcaatt taccgaagac
    14041 atgggcgcag atattgtttt cgaaacagcg ggttctgcgg tcaccgttaa acaggcacct
    14101 ttaatctggt aatgcgcggc ggtaaaatta tgattgttgg tactgtaccc ggcgattcgg
    14161 caatcaattt cctcaaaatc aatcgcgaag tcactatcca gacggtattc cgctatgcca
    14221 atcgttatcc ggtcacgatt gaagctattt cttcagggcg attcgatgtg aaatcgatgg
    14281 tgacgcatat ttacgattat cgggatgtac aacaggcatt tgaagagtca gttaacaaca
    14341 aacgcgacat tattaaaggc gttattaaaa ttagcgatta attaatacgg gagaacagaa
    14401 catgctcgca gatatcagat attgggaaaa cgatgccaca aataaacatt atgcaattgc
    14461 ccattttaac gtatggaatg cagaaatgtt gatgggcgtt aaagacgcag ccgaagaagc
    14521 gaaatccccg gttattattt cttttggtac aggttttgtc ggtaacacct catttgaaga
    14581 tttctctcac atgatggtat caatggcaca aaaagcaacg gtgccggtaa taactcattg
    14641 ggatcatggt cggagtatgg agattattca taacgcctgg actcatggta tgaattcatt
    14701 aatgcgtgat gcttctgcat ttgatttcga agaaaatatt cgtttaacca aagaggctgt
    14761 tgatttcttt catccgctgg gtattccggt agaggcggaa ttagggcatg tcggtaatga
    14821 aaccgtttat gaggaagcgc tggcgggcta tcactatacc gatcctgacc aggcggctga
    14881 atttgttgaa cgtacgggct gtgactcatt ggctgtcgcc atcggcaacc agcatggggt
    14941 ttatacgtca gagccgcaat tgaactttga ggtcgtcaaa cgcgtacgcg atgctgtttc
    15001 tgttccgctg gttttgcatg gcgcatcggg gatcagtgat gccgacatta aaactgcaat
    15061 ttcgttgggt attgcgaaaa tcaacattca tacggagctc tgtcaggccg caatggtcgc
    15121 agtgaaagaa aaccaggatc agcctttcct gcatttagaa cgtgaagtac gtaaagctgt
    15181 aaaagaacgg gcattggaaa aaattgaact gttcggttct gatggcaaag cggaataatg
    15241 atatggataa tctcgacgtt atttgtatag gtgccgctat tgttgatatt ccattacaac
    15301 cggtcagtaa aaatatcttt gatgtagatt cttatcctct tgaaagaatc gcaatgagca
    15361 ccggggggcg atgcaataaa tgaagcaaca attatttctc gtctggggca tcgtaccgca
    15421 ttaatgagcc gcattggtaa agatgcctca gggcaattta ttctcggcca ttgtcgcaaa
    15481 gagaatattg atattcaaag cctgaaacag gacgtcagca tagatacatc tattaacgtt
    15541 ggactggtga cggaggatgg cgagcggacg tttgtcacga accgtaacgg cagtttgtgg
    15601 aaattaaata ttgacgatgt tgattttgcg cggtttcctc aggcgaaatt attatcactg
    15661 gccagtattt tcaacagtcc tctattggat ggtaaagcgc taacagaaat ttttacgcaa
    15721 gcgaaagccc ggcagatgat catttgtgcc gatatgatca aaccgcggtt gaatgaaacg
    15781 ctggatgata tttgcgaagc attgagttat gtcgattatc tgtttccaaa ttttgccgag
    15841 gcaaaattac tcacagggaa agagacactg gatgaaattg ctgactgctt tcttgcgtgc
    15901 ggcgtaaaaa cggtggtgat taaaacgggt aaagacggct gctttatcaa gcgtggtgac
    15961 atgacgatga aggtgccggc ggtcgcagga ataaccgcca tcgacaccat tggcgcgggc
    16021 gataactttg cttcaggttt tattgcggca ctgttagaag gcaaaaatct gcgtgaatgc
    16081 gcacgctttg ccaatgcaac ggcggctatc tcggttttaa gcgtcggtgc caccaccggc
    16141 gtaaaaaaca gaaagctggt cgaacaattg ctggaagaat acgaaggata atgaaggcaa
    16201 atgaaaaaga tacctttagg cacaacggat ataacgcttt cgcgaatggg gttggggaca
    16261 tgggccattg gcggcggtcc tgcatggaat ggcgatctcg atcggcaaat atgtattgat
    16321 acgattcttg aagcccatcg ttgtggcatt aatctgattg atcctgcgct aggatataac
    16381 tttggcaata gtgaagttat cgtcggtcag gcgttaaaaa aactgccccg tgaacaggtt
    16441 gtagtagaaa ccaaatgcgg cattgtctgg gaacgaaaag gaagtttatt caacaaagtt
    16501 ggcgatcggc agttgtataa aaacctttcc ccggaatcta tccgcgaaga ggtagaagcc
    16561 agcttgcaac gtctgggtat tgattacatc gatatctata tgacgcactg gcagtcggtg
    16621 ccgccatttt ttacgccgat agctgaaact gtcgcagtgc ttaatgagtt aaaggctgaa
    16681 gggaaaattc gcgctatagg cgctgctaac gtcgatgctg accatatccg cgagtatctg
    16741 caatatggtg aactggatat tattcaggcg aaatacagta ttctcgaccg ggcaatggaa
    16801 aacgaactgc tgccgctatg tcgtgataat ggcattgtgg ttcaggttta ttccccgcta
    16861 gagcagggat tgttgaccgg caccatcact cgtgattacg ttccgggcgg cgctcgggca
    16921 aataaagtct ggttccagcg tgaaaacatg ctgaaagtga ttgatatgct tgaacagtgg
    16981 cagccacttt gtgctcgtta tcagtgcaca attcccactc tggcactggc gtggatatta
    17041 aaacagagtg atttaatctc cattcttagt ggggctactg caccggaaca ggtacgcgaa
    17101 aatgtcgcgg cactgaatat caacttatcg gatgcagacg caacattgat gagggaaatg
    17161 gcagaggccc tggagcgtta aatactcaac acgattcacc tctgtttctg tgaaaactgt
    17221 gatgaaaatc tgtgattcaa ataggttatt ttgaaagtaa acatcggtta tgcgataatc
    17281 gcgctaatgt gatgtaaaaa gatacagggg tgtaatcgtg gcggcaaaag acaggattca
    17341 ggcaattaag caaatggttg ccaacgataa aaaggtgaca gtctcaaatt tgagtgggat
    17401 ttttcaggta accgaagaaa ccattcgccg cgatcttgag aagctggaag atgaaggctt
    17461 tttgaccaga acctatggtg gtgctgtttt aaatacagcg gtgttgacgg agaatatcca
    17521 tttttataag cgcgcttcat cgttttatga agagaagcag cttattgcac gcaaggcact
    17581 accctttatc gacaataaaa ccaccatggc agccgattcc agtagtaccg taatggaatt
    17641 gctcaaattg ttacaggacc gtagtgacct gacgttgcta accaactcgg cggaagcgat
    17701 tcatgttctg gctcagtcag aaattaaagt cgtttcaacg ggtggggaac taaacaaaaa
    17761 tacactttcc ctgcaaggaa gaattactaa agagatcatc agccgctacc atgtcgatat
    17821 catggtaatg agttgtaaag gtcttgatat taacagcggc gcgctggact ctaatgaagc
    17881 ggaagctgaa atcaaaaaga caatgatccg tcaggcgaca gaagttgcgt tattggttga
    17941 tcactctaag tttgatcgta aagcttttgt ccagttagct gactttagtc atattaatta
    18001 tataataact gataaatcac cgggtgcaga gtggatagca ttttgcaaag acaataatat
    18061 ccagctggtg tggtaattta agcacacgtt gtcaatatta tttgttcctg cgcctggaaa
    18121 taaaatgatg ataaagatgc ggcaagtgaa tttgccgtaa ttgctacctt ttatagcaaa
    18181 tccgaggcac ccaatggaac aatatgatca aattggcgca agactggacc gcttgccttt
    18241 ggcccggttt cattatcgta tatttggtat aataagcttt agtctgttat taacggggtt
    18301 tttgagttac tcaggtaatg tcgtcttagc aaagctggta agcaatggat ggtcaaataa
    18361 tttcctcaat gccgccttta cctcggcatt aatgtttggt tatttcatcg gctcacttac
    18421 tggtgggttt attggtgact actttgggcg gcgcagggcg tttcgcataa atcttctcat
    18481 cgtcggtatt gctgcaacag gggccgcttt tgtccctgat atgtactggc tcattttctt
    18541 gcgcttcctg atgggaacag gaatgggggc gctgattatg gttggctatg cctcatttac
    18601 ggagtttatc cccgcgacgg tgcgtggaaa atggtccgcg cggctctcat ttgttggtaa
    18661 ctggtcgccc atgctgtctg cggcgatagg cgtggtggtt atcgcttttt ttagttggcg
    18721 aataatgttt cttctgggtg gtattggcat actgttagcc tggtttctct caggtaaata
    18781 ctttatcgag tcgccacgat ggctggcagg gaaagggcaa atcgccggtg cagaaagcca
    18841 acttcgtgaa gtagagcagc aaattgaaag ggagaagagt attcgtttac cccagcttac
    18901 tttgaaccag agcaacagca aggttaaggt aatcaagggt accttcaggc tcctgtttaa
    18961 aggggaaatg ttacgacgta cattagttgc gataactgtt ttaattgcaa tgaacatttc
    19021 gctttatacc attaccgtat ggataccgac catatttgtt aactccggca ttgatgtcga
    19081 taaatcaata ttaatgaccg ctgttattat gattggtgct ccggtaggaa tatttattgc
    19141 ggcattaatt attgatcatt ttcctcgtcg attatttggc tccgccttac ttattattat
    19201 tgccgtgtta ggctatatct attcaagtca gactacagag tgggcgattt taatctatgg
    19261 tctggtgatg atcttctttt tatacatgta tgtttgcttc gcgtcggcgg tttatatccc
    19321 ggagctttgg ccaacgcatt tacgcctgcg cggttcgggt ttcgttaatg ccgtcggacg
    19381 gatcgtcgca gttttcacgc cctatggcgt tgcggcatta ttaacacatt atgggtcgat
    19441 cacggtgttt atggtgcttg gtgtcatgtt agtgctctgt gcgctggttc tctccatttt
    19501 tggcatcgaa acgcggaagg tgtcgctgga agagatttct gaggtgaatt aaatctttcg
    19561 ttgatggcat caattgccag atgtaccact gacgtgtttt atttggccta caatgccagt
    19621 acagaattgt aggccagcgt aaaattaccc ctgcgtctct tcccagtctg ccagcgtata
    19681 tagcgttgcc ccagctgctg acatctccat aaacgcgtgt gcactgtcct ggggctggat
    19741 attcacgcca cgacagccat cggtaatcac gtttacctta taacctaact gtaacgcgtc
    19801 cagcacggta aacttcacgc aatagtcagt agccaggccc ataacgatca attcatcgat
    19861 ttcatgatcg cgcaaccagt catcaagcgc ggttttctgc cgacgtccgt tatcaaaaaa
    19921 ggcactgtaa ctgtcaacta aaggattttc gcctttatgg aacaccgctg cgatctcttt
    19981 ttgattcagt aacggatgta attgtgcgcc ttcactgttc tgcacacagt gttcaggcca
    20041 gaaagtttgc cgcaggccgt cgagttggcc tggcgtataa ggctctacgc cgtgctggct
    20101 ggcaaagctg ccgtgattcg ccggatgcca gtcctggctg gcgataaccg cttctccgcg
    20161 cgactggcac cagtcaataa ggcggttagc aacctccacc gtactgtcac cttccggtac
    20221 ggcgagcgcg ccaccagcac agaaatcatt ttgtaaatcg accagtaaca gggcgcgatg
    20281 gggcattgca gtctccttaa tcatccggcg tcagttcgcc gcgcaggttt tggctcatgg
    20341 ccttgcgaat ggtttcagta tccagttcct ggctcagcag gtaatgcagt ttggttagcg
    20401 tggcttctac agtcatatct gcaccgccaa taacgccggc atgggcgagg acgttaccgg
    20461 tggcataacc actcatattc actttaccgg acatacattg tgtcaggttg accaccacaa
    20521 taccgcgatc gctggcttct tgtaattcct gcaggaaggc tttgttttgt ggcgcattac
    20581 ccacgccata ggagcgcaga atcaatgctt tcaccggttg gcgcagaaaa ttgcgcacga
    20641 cgtcagcaga aatccctgga taaatcgtca ctacgccaat tggttgtggc gtgattggat
    20701 gaacgattaa ttccccttca ccgtgcgggg cgggtggcgt attcaaacga cgaatatgga
    20761 taccagcttc cagtaacgga ggaaggtttg gagaggcaaa cgcatcaaaa ccatcggcat
    20821 gggctttggt agtgcggttg ccgcgataca atcggttatt gaaaaagagc gttacttcgt
    20881 tgatcggata attcgccgca acgtacaacg cattcagcag attaatttgt ccgtcagagc
    20941 gtaactcagc cagcgggatt tgtgaccctg tcacaatgac cggtttaccg agattctcga
    21001 gcatgaacga cagcgcagag gcggtatacg ccatcgtgtc ggtaccgtgt agaatgacaa
    21061 aaccatcata gtcgtcatag tgcgctttaa tatcttcagc aatatgctgc cagtcttccg
    21121 gcgtcatatc agaagaatcc atcaacggcg tatattcatg aatggtgaaa tctggcatct
    21181 ccaggcgatg gaattccggc atcagcgcca gttggcgttg tagatgacct gacaccggaa
    21241 tataaccctg ctcggaacgc tgcatcccga tggtcccgcc cgtgtaggcg acgtaaattg
    21301 atttcttttg catgatattg atatgaatga tatcgaaaga gcaaaagtat atacgtgagg
    21361 caggaggagc gcacctgata ctatgcggat aaggaaatat ggggcgagac agcgggaaga
    21421 ggattcgtcg aggtaaaaaa taatgtggcc gacgatctgt cggccactca gtacaagact
    21481 taacgcacgt tggcgcaggt caggcaaaac gcataacggt tttgtgggtc gttaaacgcg
    21541 gccagcttgt cgctttcact tttaacggta gaggctaccg aggcaagcgg tgcaggtaac
    21601 atggcctgga acgcatctgg caacattgcc cggacagaac cagacatgtt gtccatcact
    21661 ttgtcgaaga aggtcggttc atcaacgtag tattccagat gccactgttt cacttttgcg
    21721 agctctgctg ctttggcaac cgcatcatcg aaatccccga gactatcgac cagcccgtta
    21781 gcttttgcat cctgaccggt ccagacgtgg ccctgggcga ttttatcaat ttgctccggc
    21841 gtcgaatgac gcgcatcagc aaccagcgtg ataaagcgtt tatagccatt ctcaatgctt
    21901 aattgcatca tctgctgcgc ttccggcggc agtgccctgg tgatagaaac atccgccagc
    21961 ggtgaagttg agacaccatc agtatgaaca ccaatcgaat ccagactatt ttctacggtg
    22021 gtgatcacgc cgaagatacc gatagaaccg gtcagggtgc tggggttagc cacaatgtaa
    22081 ttagctggcg tggaaatcca gtaaccacca gatgccgcca tgccgcccat cgatacaacc
    22141 acagacttac ccgctgcccg ggctgctgcc agttcagcgc gaatcacttc agacgcggta
    22201 acgctgccgc ctgggctatt aacacgcagg acaatcgctt tcactttcgg gtcaaggcga
    22261 gcgtcgcgga tttgtgccgc agtggtatca ccgccaacat tcccctgagt ttcctcgcca
    22321 tccataattg cgccattagc gaagacgaca ccgatgctgt caccggtatc tgccggcgtt
    22381 ttcaatgcgt aatcgtaata actgatggcg cgataatttt tatcagtctt actccagccg
    22441 aactctttgg tcagagcttt ttcgatttcg gcactcgatg ccagtgcatc gaccagcttg
    22501 ttttccagtg catatttcgc ggtatcgcca ccggttttgg ttaaaccctc aagcaaccct
    22561 tgcgcgccag ggaatacctg ctgagcaggg atctgccggt tagcggcaac agtattcaga
    22621 tagttttgcc acagctcacc aatccagcgg ctgtcagctt cgcgggctgc cggtgacata
    22681 tcatcacgaa taaacggttc aacggcagat ttatacgtac ccacgcggaa cacatgggtg
    22741 gaaactttca gcttatccag caacgatttg tagtacagac cgttggtagc aaaaccgtgc
    22801 agatcaacca cgccttgcgg tgacagccaa attttattgg cgaaactggc gagataatat
    22861 tgcccctggc tgtagttctc gccaacggca taaaccggtt tcccgctgtc acgaaactct
    22921 ttcagggctt tgccgatgta ctgcatagac ggctggtcgc cgcctgcgaa gtttttcaga
    22981 tccatcacga taccggtgat attgcggtcg tccttcgcct ggcgaatggt gttgacgata
    23041 tcaaacagtg agttttcctg cagacgatcg gaactggcac caagcagctg gcggcttaat
    23101 ttactaaaac gctgagaact gtctggttta tcgacgatca caccagaaat gtccagcagc
    23161 agtgcgccac gactggccgt ttctttcgaa tcaccaccac tgacctgcat ccagatcccc
    23221 acaccaacca gtacgaggaa aataaagaac aggttaagta ccatttcacg gacgaaattc
    23281 agcagacgcc acgtccattt aaaaaatccg gcaataaatc gccaaagggt tcgcatgtat
    23341 tctcccaact taaggtcaca cctgtcacag gcgcaataga tgtggttatc gtaatgaccc
    23401 aaccggcact tgtcagcagg aattgcggac tgtgctgtaa caaaatccac tctcgtgtta
    23461 attttgtgaa taaatatcac gacaggagtt aatcaaatgg atgcactcga actattgatc
    23521 aatcgccgta gcgcctcccg cttggctgaa cccgcgccaa cgggtgaaca actgcaaaac
    23581 atcctgcgtg cgggtatgcg tgcgccggac cataagtcca tgcaaccgtg gcattttttt
    23641 gtgattgaag gggaagggtg cgagcgtttc agcgccgtac tggaacaggg ggcgattgct
    23701 gccggtagtg atgataaagc tatcgacaaa gcccgtaatg cgccgttccg cgcaccgctc
    23761 atcatcacgg tggtggcgaa atgcaaagaa aatcataaag tcccgcgatg ggaacaggaa
    23821 atgtctgccg gatgcgcggt catggcgatg caaatggcag ccgttgccca gggatttggc
    23881 ggcatctggc gcagtggcgc attaactgaa agtccggtag tgcgtgaagc attcggttgc
    23941 cgtgagcagg ataaaattgt cggttttctc tacctcggca cgccgcagtt gaaagcatct
    24001 acgtcgatta acgtcccgga cccgacgccg tttgtaactt atttctgata attgtcgcga
    24061 aactgtctgg attgtgagca aaggcgcagg aattcagaca ctctcactta tcacttcacg
    24121 gaatgagggt taccataccc ggattgcatt gacaggagat gtccatgagc gagaactcga
    24181 ttcgtttgac ccaatacagc cacggagctg gttgcggctg taaaatttcc ccaaaagtgt
    24241 tggaaaccat cctgcacagt gagcaggcga agtttgttga tccgaatttg cttgtgggta
    24301 atgaaacccg cgacgatgcg gcggtgtacg atctgggcaa tggcaccagc gttatcagta
    24361 ccaccgactt ctttatgccg atcgttgata atcctttcga ttttggccgc attgcggcga
    24421 ctaacgccat cagcgatatc ttcgcgatgg ggggcaaacc gattatggcg attgcgatcc
    24481 tcggctggcc gattaacaaa ctttccccgg aaattgcccg cgaagtaacc gaaggtggac
    24541 gctatgcatg ccgtcaggcg ggtattgcgc tggctggcgg tcactccatc gatgcgccgg
    24601 agccgatttt tggtctggcg gtaacgggga tcgtaccgac cgagcgggtg aagaaaaaca
    24661 gtaccgcaca agccggatgc aaactgttcc tgacgaaacc gctggggatc ggcgttctta
    24721 ccacggctga gaaaaaatca ctgttgaaac cagaacatca gggactggcg acggaagtga
    24781 tgtgccggat gaacatcgca ggcgcgtcct ttgccaacat cgaaggcgta aaagcgatga
    24841 ccgacgttac gggctttggt ctgctgggcc acttgagcga aatgtgtcag ggggctggtg
    24901 tgcaggcacg cgtcgactat gaagcgatcc cgaaactccc cggtgttgaa gagtacatta
    24961 agttgggcgc agtacctggc ggcactgaac gtaactttgc cagctacggt catctgatgg
    25021 gtgaaatgcc gcgtgaagtg cgcgatctgc tgtgtgatcc gcaaacttct ggcggtttgc
    25081 tgctggcggt catgccggaa gcagaaaatg aggttaaaac tacagccgcc gagtttggca
    25141 ttgaactgac ggcgattggc gaactggtgc cagcgcgcgg cggtcgtgcc atggttgaga
    25201 tccgttaatt caatgcggtt gtttattgcc gaaaaaccga gtctggcgcg cgccattgct
    25261 gatgtcctgc ccaaaccgca ccggaaaggc gatggcttta tcgagtgcgg taatggtcag
    25321 gtggtgactt ggtgtatcgg tcacctgctt gagcaggcgc agccagacgc ctacgacagc
    25381 cgctatgcgc gctggaatct tgcggatttg ccgattgtcc cggaaaagtg gcaattacag
    25441 ccacgaccct ccgtgaccaa acaacttaac gtcatcaaac gcttcctgca tgaagccagc
    25501 gaaatcgttc acgccgggga cccggatcgt gaagggcaat tgctggtgga tgaagtgctg
    25561 gactatctgc aactggcacc ggaaaagcgc cagcaggtgc agcgttgctt gataaacgac
    25621 ctgaacccgc aggcggttga gcgggcgatc gaccgtcttc gttccaacag tgagtttgtg
    25681 ccattgtgcg tttctgcgct ggcgcgagcg cgtgccgact ggctgtacgg catcaatatg
    25741 acccgtgcgt ataccattct cggtcgcaat gccggttatc agggcgtact ttccgtggga
    25801 cgcgtgcaga cacccgtgct cgggctggtg gtgcgccgcg atgaagagat tgaaaacttc
    25861 gtggcgaaag acttctttga agtcaaagca catatcgtga cacctgccga tgagcggttt
    25921 accgctatct ggcaaccgag cgaagcgtgt gaaccgtacc aggatgaaga agggcgcttg
    25981 ttacatcgtc cactggcgga gcatgtggtt aaccgcatta gtggtcaacc ggctattgtc
    26041 accagctata acgataaacg ggaatcagaa tccgcgccgc tgccgttttc gctttcggcg
    26101 ttgcagattg aagcggcaaa acgcttcggt ctgagcgcgc agaacgtgct tgatatctgc
    26161 cagaagctgt acgaaacgca caagctaatc acttatccgc gttccgattg tcgctatttg
    26221 ccagaagaac attttgccgg acgccacgcg gtgatgaatg ccatcagcgt tcatgcaccg
    26281 gatctgttgc cgcagccagt ggtagatcca gatatacgca accgctgttg ggatgacaaa
    26341 aaggtcgatg cgcaccacgc catcatcccg accgcacgga gttctgcgat caacctgacg
    26401 gagaacgaag cgaaggtcta taacctgatt gcccgtcaat acctgatgca gttctgcccg
    26461 gatgcggtgt tccgcaagtg tgttatcgaa caggacattg ccaaaggcaa atttgtcgct
    26521 aaagcccgtt ttcttgctga agcaggctgg cgcacgctgt taggcagcaa agagcgcgat
    26581 gaagaaaacg acggcacgcc attgcctgtg gtggcgaaag gcgatgagtt gctgtgtgaa
    26641 aaaggtgaag tggtagagcg gcaaacccag ccgccgcgcc attttaccga tgcaacactg
    26701 ctttcggcga tgaccgggat cgcgcgcttt gtgcaggaca aagatctgaa aaaggtcctt
    26761 cgtgcgaccg atggtctggg gacagaagca acccgtgccg ggattattga actgctgttc
    26821 aagcgtggtt ttctgaccaa aaaagggcgc tatatccact ccaccgacgc cggaaaagcg
    26881 ttattccatt cgctgccaga aatggcgacg cgaccggaca tgaccgcgca ctgggaatcg
    26941 gtgctgacgc aaatcagcga aaagcagtgt cgctatcagg actttatgca gccgctggtg
    27001 gggacgctat atcagcttat tgatcaagcc aaacgtacgc cggtgcggca gtttcgcggc
    27061 attgtggctc cgggcagtgg tggcagtgct gataagaaaa aggctgcacc gcgtaaacgt
    27121 agtgcgaaaa aaagtccgcc agcagatgaa gccggaagcg tggcgatagc gtaagcgagc
    27181 gaatctttcg tgctattcga gtcatattct gaaatatcca gcggatcaag aaaattcgtt
    27241 ggatattttt ttgcatggat aaaattatcg cctctaaagt atgtaataac agggaatgtg
    27301 atgaaaaaag ttttattaca aaaccatcct gggagcgaga agtattcttt taatggctgg
    27361 ggaatattta atagtaattt tgaacgggtg attaaagaaa ataaggccat gctgctttgt
    27421 aagtgggggt tttatttaac atgtgttgtc gctgtaatgt ttgtattcgc agcgataaca
    27481 tccaacggtt tgaatgaaag aggcctgatt accgcgggat gctcttttct ttatctatta
    27541 attatgatgg ggcttattgt tcgggccggt tttaaagcaa aaaaagaaca actacattat
    27601 tatcaggcta aaggtattga gccgctcagt atcgagaagt tacaggcgct acaattgatc
    27661 gcaccttatc gattctatca taagcaatgg tctgaaacgc tagagttctg gccgcgaaag
    27721 cctgaacctg gcaaagatac cttccaatat catgtgcttc cttttgactc gatcgatatc
    27781 atcagtaaaa gacgcgagtc tttagagaat caatggggta tcgaagatag cgaaagttat
    27841 tgtgccttaa tggagcattt tctttctggc gaccatggag ccaatacctt taaagcaaac
    27901 atggaggaag ccccagagca ggttatcgcc gtgttgaata aatttgctgt ttttccctca
    27961 gactatatct ctgattgcgc taataatcgc tccggtaaat cctcggcgaa gctaatatgg
    28021 gcggcggaat tatcatggat gatctcgata tcaagcacag cttttcaaaa cgggacaatt
    28081 gaagaagaac tggcctggca ttatataatg cttgcttctc gaaaggcgca cgagttgttc
    28141 gaaagcgaag aagattatca aaaaaatagt ctaatgggat ttctttactg gcatatctgc
    28201 tgctatcgca gaaagttaac ggatgctgaa cttgaagcgt gttatcgtta cgacaagcag
    28261 ttttgggagc actacagtaa gaaatgccgt tggcccataa gaaatgttcc ctggggagca
    28321 tcatccgtta aatactcata aacgcctgaa attttgccgg gggcgctgcg cttgcccggc
    28381 ctacattaga gcgtaactgc aatgaattgt gcccatctgt aggcctgata agcgtagcgc
    28441 catcaggcat ttacaactta aatcacaccc tgcgccagca tcgcatcggc aaccttcaca
    28501 aaaccggcaa tgttcgcgcc ctgcacgtag ttggtttgct caccttcacc accatgctca
    28561 acacaggcat ggtggatatc cagcatgatg tgatgcaaac gtgcgtcaac tttctcggct
    28621 ttccagccca ggcgcgcagc gttttgtgcc atttccaggc ccgacgttgc tacaccgcca
    28681 gcattagccg ctttacccgg tgcaaacagt acgcctgcct gctggaacag ttcagtcgct
    28741 tcgatggtgg tcggcatatt tgccccttca gcgacggctt taacgccatt ggcgataagc
    28801 tgatgcgcgg tgtcaacatc cagttcgttc tgggtggcgc aaggcagggc gatatcaacc
    28861 ggtacagacc acggctgttg gccttcgaga tagaccagac caaattcttt ggcgtaatct
    28921 gccactcgac catcgcggct ggctttgatt tcgataagac gtgccagttt ctctttcgtg
    28981 aatccgcttt catcaactac agtgccgctg gagtctgacg cagtgatcgc acgagcacca
    29041 aattccatcg ctttttcgat agcgtactgg gcgacgttgc cggagccaga aacggaaacg
    29101 cgcatccctt caaaacccat accgtggcgt tttaacattg cttctgtgaa ataaaccaga
    29161 ccgtagccgg tagcttccgg gcgaataaga ctaccgccaa atgaaaggcc cttaccggtg
    29221 aagacgcagg cggtattgtt ggagagcttt ttcatcatcc ccgccataaa gccgacttca
    29281 cgaccaccaa cgccgatatc acctgccgga acgtcggtat ccgcgcccag gtggcgatac
    29341 agttcagtca tcagcgcctg gcaaaaacgc atcactttac cttcgctttt tcctttcgga
    29401 tcgaaatcgc tgccgccttt accaccgccc atcggcaggg tagtcagggc atttttgaag
    29461 gtttgttcaa agccgaggaa tttgagaatg gaaaggttaa ctgacggatg gaagcgcata
    29521 ccgcctttgt acgggccgat ggcagagctg aactgcacac gccatgcacg gttgacctgt
    29581 atctggttgc gatcatcaac ccataccacg cgaaactgga tcacgcgctc cggttcaacc
    29641 agacgctcca gtaatgacat ctggcgatat tttggatttt gttcaagaaa aggccagagt
    29701 gtggtcatta cttcacgaac ggcttgcgcg aactcggttt gattcgggtc gcgcttttgg
    29761 acatggttga ggaatgactc cagagaatat gtctgat
//
LOCUS       DAJTYG010000044        29418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTYG010000044 DAJTYG010000000
VERSION     DAJTYG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.044"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..109)
                     /locus_tag="OO568_002089"
     CDS             complement(<1..109)
                     /locus_tag="OO568_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter ATP-binding protein"
                     /protein_id="HCR6641443.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVH"
     gene            complement(179..1168)
                     /gene="araF"
                     /locus_tag="OO568_002090"
     CDS             complement(179..1168)
                     /gene="araF"
                     /locus_tag="OO568_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCR6641444.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAH
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1965..2468
                     /locus_tag="OO568_002091"
     CDS             1965..2468
                     /locus_tag="OO568_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6641445.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2548..2799)
                     /locus_tag="OO568_002092"
     CDS             complement(2548..2799)
                     /locus_tag="OO568_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6641446.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2914..3000)
                     /gene="azuC"
                     /locus_tag="OO568_002093"
     CDS             complement(2914..3000)
                     /gene="azuC"
                     /locus_tag="OO568_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6641447.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            3263..3586
                     /locus_tag="OO568_002094"
     CDS             3263..3586
                     /locus_tag="OO568_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6641448.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3757..4254
                     /gene="ftnA"
                     /locus_tag="OO568_002095"
     CDS             3757..4254
                     /gene="ftnA"
                     /locus_tag="OO568_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6641449.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(4291..4530)
                     /locus_tag="OO568_002096"
     CDS             complement(4291..4530)
                     /locus_tag="OO568_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6641450.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            4720..5931
                     /gene="tyrP"
                     /locus_tag="OO568_002097"
     CDS             4720..5931
                     /gene="tyrP"
                     /locus_tag="OO568_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6641451.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVPSPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(5993..6658)
                     /gene="yecA"
                     /locus_tag="OO568_002098"
     CDS             complement(5993..6658)
                     /gene="yecA"
                     /locus_tag="OO568_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6641452.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(6854..6940)
                     /locus_tag="OO568_002099"
     tRNA            complement(6854..6940)
                     /locus_tag="OO568_002099"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6904..6906),aa:Leu,seq:taa)
     gene            complement(6953..7026)
                     /locus_tag="OO568_002100"
     tRNA            complement(6953..7026)
                     /locus_tag="OO568_002100"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6992..6994),aa:Cys,seq:gca)
     gene            complement(7308..7856)
                     /gene="pgsA"
                     /locus_tag="OO568_002101"
     CDS             complement(7308..7856)
                     /gene="pgsA"
                     /locus_tag="OO568_002101"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6641453.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(7913..9745)
                     /gene="uvrC"
                     /locus_tag="OO568_002102"
     CDS             complement(7913..9745)
                     /gene="uvrC"
                     /locus_tag="OO568_002102"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6641454.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(9742..10398)
                     /gene="uvrY"
                     /locus_tag="OO568_002103"
     CDS             complement(9742..10398)
                     /gene="uvrY"
                     /locus_tag="OO568_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6641455.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            10694..10870
                     /locus_tag="OO568_002104"
     CDS             10694..10870
                     /locus_tag="OO568_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641456.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            10857..11081
                     /locus_tag="OO568_002105"
     CDS             10857..11081
                     /locus_tag="OO568_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6641457.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLILQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(11114..11734)
                     /gene="sdiA"
                     /locus_tag="OO568_002106"
                     /pseudo
     CDS             complement(11114..11734)
                     /gene="sdiA"
                     /locus_tag="OO568_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(11964..12716)
                     /gene="yecC"
                     /locus_tag="OO568_002107"
     CDS             complement(11964..12716)
                     /gene="yecC"
                     /locus_tag="OO568_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6641458.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(12713..13381)
                     /gene="tcyL"
                     /locus_tag="OO568_002108"
     CDS             complement(12713..13381)
                     /gene="tcyL"
                     /locus_tag="OO568_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6641459.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(13396..14382)
                     /gene="dcyD"
                     /locus_tag="OO568_002109"
     CDS             complement(13396..14382)
                     /gene="dcyD"
                     /locus_tag="OO568_002109"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6641460.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(14487..15287)
                     /gene="tcyJ"
                     /locus_tag="OO568_002110"
     CDS             complement(14487..15287)
                     /gene="tcyJ"
                     /locus_tag="OO568_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6641461.1"
                     /translation="MKLAHLGRQALMGVMGVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(15375..15926)
                     /gene="fliZ"
                     /locus_tag="OO568_002111"
     CDS             complement(15375..15926)
                     /gene="fliZ"
                     /locus_tag="OO568_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6641462.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(15972..16691)
                     /gene="fliA"
                     /locus_tag="OO568_002112"
     CDS             complement(15972..16691)
                     /gene="fliA"
                     /locus_tag="OO568_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR6641463.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDAPQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(16856..18508)
                     /gene="fliC"
                     /locus_tag="OO568_002113"
     CDS             complement(16856..18508)
                     /gene="fliC"
                     /locus_tag="OO568_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6641464.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIILQAGNSVLAKANQVPQQVLSLLQG"
     gene            18774..20180
                     /gene="fliD"
                     /locus_tag="OO568_002114"
     CDS             18774..20180
                     /gene="fliD"
                     /locus_tag="OO568_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6641465.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASRNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            20205..20615
                     /gene="fliS"
                     /locus_tag="OO568_002115"
     CDS             20205..20615
                     /gene="fliS"
                     /locus_tag="OO568_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6641466.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            20615..20980
                     /gene="fliT"
                     /locus_tag="OO568_002116"
     CDS             20615..20980
                     /gene="fliT"
                     /locus_tag="OO568_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6641467.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            21058..22545
                     /gene="amyA"
                     /locus_tag="OO568_002117"
     CDS             21058..22545
                     /gene="amyA"
                     /locus_tag="OO568_002117"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6641468.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(22579..22992)
                     /locus_tag="OO568_002118"
     CDS             complement(22579..22992)
                     /locus_tag="OO568_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6641469.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            23179..24384
                     /gene="yedE"
                     /locus_tag="OO568_002119"
     CDS             23179..24384
                     /gene="yedE"
                     /locus_tag="OO568_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6641470.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            24381..24614
                     /gene="yedF"
                     /locus_tag="OO568_002120"
     CDS             24381..24614
                     /gene="yedF"
                     /locus_tag="OO568_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6641471.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            24721..25391
                     /locus_tag="OO568_002121"
                     /pseudo
     CDS             24721..25391
                     /locus_tag="OO568_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(25431..26639)
                     /locus_tag="OO568_002122"
                     /pseudo
     CDS             complement(25431..26639)
                     /locus_tag="OO568_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            26707..27322
                     /gene="tnpA"
                     /locus_tag="OO568_002123"
                     /pseudo
     CDS             26707..27322
                     /gene="tnpA"
                     /locus_tag="OO568_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            27650..28732
                     /locus_tag="OO568_002124"
     CDS             27650..28732
                     /locus_tag="OO568_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR6641472.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(29221..>29418)
                     /locus_tag="OO568_002125"
     CDS             complement(29221..>29418)
                     /locus_tag="OO568_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6641473.1"
                     /translation="SEGISFQSILDDVRLNNALSAIQTTVKPISEIARENGYKCPSRF
                     TERFHNRFKITPRELRKASRE"
BASE COUNT         7421 a         7405 c         6885 g         7707 t
ORIGIN      
        1 catgaacctg accggcatag cagtcaaaac taatatccgt cagcgcctta acgccgggaa
       61 acgttttacc gatgccgcga aatgagagat acggggtaga ctgttgcata acgtctccgt
      121 gaatcactgg tcgttcacac cccctctgca tcatgcagag gggaattttt ccggcaaatt
      181 acttaccgcc taaacctttt ttctccagtt cttctttaaa gttgtcacgc gtgatcagta
      241 ccacgtctgt aacttcggta aattttggcg tttcaacgtc ttttgctacc cagttgtaaa
      301 gcatttcgct ggatttatag ccatgtacgt ccgggcttgg cagcagggaa ccgtagaagc
      361 cggttgcctg tgctttagac agttcgctta ctgcatccac accgttaata ccaataccga
      421 tgatatcggc tgctttaaag ccctgacctt cggttgcacg tacgccgcca agtacggtgc
      481 tgtcgttcat accgacgatc agccaatgtt taacttccgg atgttgaacc agcattgagt
      541 tggcagcgtc aaatgccccc gggatgtcgt tagatttggt aggtacctga taaatttgtt
      601 tttccgggaa tcctgcagct ttcagtgcgt ccatagaacc cgttgtacgg cggcgggcgg
      661 tatccagttc gttggcggta atcgccatca ctgcgctttc tttgacatcc cagccacgtt
      721 tctgcatctc tttatacagt tcctggccct gacgttcgcc aattttagtc gccgccatca
      781 tcaccagagg aacggtatcc attggcttac ctttggcgtt aacaaactgg tcatccactg
      841 caatgacttt catatcgtag ccatgcgctt tcgcgacgat ggcagagccg agtttcgggt
      901 ccggagtaca aataacgaaa ccttttgcgc cactggctgc caggctgtcg atcgcgttca
      961 atgttttttc gccatccggc acggcaatct taataacctc aaaccctaaa tccttcccgg
     1021 ctttatcggc aaacttccat tcggtctgga accacggctc ttccggttgc ttcaccagaa
     1081 aaccgagctt caggttctcc gccatagcgg attgtgacat aacggctgcc agaccaatgg
     1141 ctgccagggc tttagtaaat ttgtgcatgg ttctctccag ctttagtgtc gttttgtgta
     1201 gggcaaaaac gaatgacatt cgttaaatta atcggaaaac aaagcattac cttttatcta
     1261 aaagataagt gaccgtgttg acatagtttt agcgagaaat taattctcca taggagagca
     1321 atatcacatc gcagaattac agtgagaacg tgcataaatt tagcgggaaa agacataagg
     1381 aaaagccaat ttgtcagaca aattgttgaa tgcacagcag attaatccat aagattagcc
     1441 tggaaatcct tgttgtcttc ggtacccatg cgggatgtct tctttttaac cagtcaatag
     1501 gccgcattac ctggcgttga gtttttgaaa tggtgtaata accacaactc aaagatgtgg
     1561 aaaatgcgcg tcattcattt cgccattaat tatcactgtg ctcattaatt aacagaacac
     1621 gtataatgag agccatctcg caaaaatgaa aaaacgtttt ataaaatcat cacttcatca
     1681 tgaattcaaa ttcatcgatt aatatcaaca agatacaaaa agcactatca ttaaaattca
     1741 ttgcatttac attgatttca ccaatgaaat gtaaaaatat ataaacttga tgatttaagc
     1801 attttcttat aaccgttcag acgttattct tatttcagat catcgtcaga attgactcca
     1861 cgatcacatt tcggaccggc agaaaggaat tattctgcaa acagtaatta tggtgttttg
     1921 atttatcttg cacctctcca cttctggata taaggatatt aggtatggca accgctggaa
     1981 tgcttctcaa actcaactct caaatgaacc gcgagtttta cgcatccaat ctctaccttc
     2041 acctgagtaa ctggtgttct gaacagagtc tgaacggcac cgccactttc cttcgcgccc
     2101 aggcacagag taatgtgacc caaatgatgc gcatgtttaa ctttatgaag agtgtcggcg
     2161 ctacccccat cgttaaagcc attgatgttc caggtgaaaa actgaactct ctggaagaac
     2221 tgttccaaaa aacgatggaa gaatacgagc aacgttcaag cacgctggcg cagttagccg
     2281 atgaagcgaa agaactgaat gatgattcaa ccgtcaattt cctgcgcgat ctggaaaaag
     2341 aacagcagca tgatggcctg ttgctgcaaa ccattcttga tgaagtgcgc agtgcgaaac
     2401 ttgcgggtat gtgccctgtg cagaccgacc aacatgttct gaatgtcgtg tcacaccagc
     2461 tgcattgatc atacatcggc gctaatacat tgcgccgatg aagtttttga gaaaccgctg
     2521 cctcatctgt ttgaagcagc ggttttttta atgggattca ccctgtgggg taaacttgag
     2581 ttcaatcagc gcgatggctt tttggattgc ccgcatggtg accgggtctg cggcagcggg
     2641 atgatgagta aagtcgatat tcttcagctg actggacatt ttttcacgaa cttcaacggg
     2701 cgcgattaca tcgagaacat ccagaatttg tttgataacc aactggcaag ctaccacatc
     2761 agaaaccagt tcctgatcgg cattcagcgg ctgggacatc gtaaactcct gatagcattt
     2821 tgaaagccgt tatagtagcg gcttcacatc ttcagcgata gtcacatcca ccgtcatcag
     2881 gacacaaaaa aacctgccgg agcaggtttt ttgttatcgg aacatattgc ctggcggtac
     2941 gtctttgaac gtcttgcaat agttattgaa catacttttc aggattttgc gcagtttcat
     3001 cgcggcactc cgaccatttg ttatacaggt gttattgtct ttgcgcttat aatatgacaa
     3061 ccatcacaaa aatcaatctt tatgtgatac aaatcacata aatacccctt taatgttata
     3121 aaaatgataa tcaaaaaaca gcccccctat ttctgacacc tacagatggc aagaaatagc
     3181 gcctgccagg catcttttcc ggccattgtc gcagcgctgt aacgcgtaaa atagtgcttt
     3241 ctcttactct tctggctgga ccatgagact tctgattctg actctttcat taataacgct
     3301 ggcggggtgt acggttactc gccaggccca tgtgagcgaa gttgacgcgg caacaggtat
     3361 tgtacggttg gtttatgacc aggctttttt gcagcatgcc cataccgatc gttatgtcag
     3421 tcgcgggatc gccgatcgcg catgccagca ggaaggctat acccacgcga tcccctttgg
     3481 tcagccagta ggcaactgta gcctttttgc gggttcctta tgtttgaata cggaattcac
     3541 tttgtcatat cagtgccatc attccgcctt ccctgtcttt ctctaaatat tttaatcagc
     3601 gaggggatct tcgctgatta aagaaatagc ggtaatgcgt tttattccca ttcgtatttt
     3661 taataattga agtttatatt ttaccttttg caaataataa aataacaaat tatagtgacg
     3721 ccacagcaac aaatataacc tttgtggagc actatcatgc tgaaaccaga aatgattgaa
     3781 aaacttaatg agcagatgaa cctggaactg tactcttcac tgctttatca gcaaatgagc
     3841 gcctggtgca gctatcatac cttcgaaggt gctgccgcgt tcctgcgccg tcacgcccag
     3901 gaagagatga cgcatatgca gcgtctgttt gattacctga ctgataccgg caatttaccg
     3961 cgtattaata ccgttgaatc tccgtttgct gaatattcct cacttgatga attattccag
     4021 gaaacctata aacacgaaca attaatcacc cagaaaatta acgaactggc tcatgctgca
     4081 atgaccaatc aggactaccc aacatttaat ttcctgcagt ggtatgtttc tgagcagcat
     4141 gaagaagaga aactgttcaa atcgattatt gataaattaa gcctggcagg caaaagcggc
     4201 gaaggtctgt attttatcga caaagaactc tctaccctcg acacacaaaa ctaatgctct
     4261 ccggcggcag gtttctgccg ccgtttcaaa tcagtgacgg caaatcttac tctgatcggt
     4321 cgaaaaacct tcttctgaag gtataaattt gccttttgct gccagaaacg ctaccagctc
     4381 tcccgctgtc atcccttctg ccgagcacgt gtgaaaacgt gcctgttcac caaaacgcgc
     4441 tttaatcgca gcttccagac tggcatgcgt atattgctcg cctgattcaa tcatcatatt
     4501 taacacttca tgaccgtgaa tagagtccat cgttcctcct caaaaaaagc ctagcgtagc
     4561 gattaccgct tatgaagact ttgcgccagc gcaggactga atgcttttat tgtacattta
     4621 tatttacaca atatgtaacg tcggtttgac gaagcagccg ttatgcctta acctgcgccg
     4681 cagatatcac tcataaagat cgtcaggaca gaagaaagcg tgaaaaacag aaccctggga
     4741 agtgttttta tcgtggcggg aaccacaatt ggcgcaggca tgctggcaat gccgctggct
     4801 gcggccggtg ttggttttag cgttacgtta atcttgttga ttgggctttg ggcgttgatg
     4861 tgctacacgg cgctattact gctggaggtg taccagcatg ttccggcaga taccggtctg
     4921 ggcacgctgg caaaacgcta tctgggacgc tacggtcaat ggctgacggg cttcagtatg
     4981 atgttcttaa tgtatgctct gactgcagca tacatcagcg gtgccggtga attgttggcc
     5041 tccagcatca gcgactggac aggtatttca atgtcggcaa ccgctggcgt gctgttgttc
     5101 acttttgttg ccggtggcgt ggtttgtgtt ggaacatcgc tggtcgattt atttaaccgt
     5161 tttctgttca gcgccaaaat tatttttctg gtggtgatgc tggtattgct gctaccgcat
     5221 attcacaaag tgaatctttt aaccctgccg ttgcaacagg ggctggcact gtctgcaatc
     5281 ccggtgattt ttacgtcgtt tggttttcac ggtagcgtgc cgagtattgt cagctatatg
     5341 gatggcaaca ttcgtaagct acgctgggtg tttataatcg gtagtgccat ccccctggtg
     5401 gcatatattt tctggcaggt ggcgacgctt ggcagcattg attcaacaac ctttatggga
     5461 ttgctggcta atcatgctgg attaaacggg ctgttacagg cgttacgcga aatggtgccc
     5521 tctccgcatg ttgagctggc ggtgcattta tttgctgatt tagccctcgc cacgtcattt
     5581 ctcggcgttg cgttaggctt atttgattat ctggctgatt tgtttcagcg ttcaaatacc
     5641 gttggtggac gattgcaaac tggtgcaatt acctttctgc cgccgttggc gtttgcactg
     5701 ttttatccac gaggatttgt gatggcgctg ggttacgccg gtgtggcgct ggcggtactg
     5761 gcattgatta tcccttcact attgacctgg caaagcagaa agcacaatcc tcaggcgggt
     5821 taccgggtca aaggtggtcg tccggcgctg gtggtggtgt ttctctgtgg tattgctgtg
     5881 attggcgtgc aatttttgat tgcggcaggg ttgttaccag aagtggggtg atcagatagc
     5941 ctcaaattcc ttattgggtg ccagaattaa cgctgacacc caatgtgacc tcttaatgca
     6001 ggcagcactg cttaaatttc ttaccactgc cgcacggaca aggatcgtta cgccccggtt
     6061 tctcttctgc tttgatcggt tgctgaacag ctttttcctg cggatgcgcc atccagtatg
     6121 catgtaaatc aagcgccgcc agtcgaatgg catctacgct ctcttcaaac gcttctggcg
     6181 acattttctc tacccgctcg aagttttcct cagtaccgtg cagcgcaatc gcctccagcg
     6241 ctggttttaa agaatcgggc aacgttgacc agtcagaaag tgccacgccc cgcatatagc
     6301 caaagcacca ctcctcaaca atcgtcagct cgctgccatc aacttctcgc aagccgaata
     6361 acggctcaaa ctgctccggg aattcgttca gacgctctgc ggtatcggcc atatgttgaa
     6421 aagccaggtt cataaagcgc gtcatctctt tctctgacgc ccaacgcggc acatagtcag
     6481 ccccacccca cacggcaacc agccactgtt ctggttcaat ctcttgcgga gaactcaaca
     6541 ccgccgtcaa taaaccgtcc agctccgcca catcaaggat ggcgtggtca gtgttgtatt
     6601 tggtcagaat atcgtccagc cattccaact cactttcgtt taacggtccc gttttcatac
     6661 gcttttcctt gtggatctca actcgccagc acctatctta catgccggtc cgtatcagag
     6721 atactttttg agtggctttg ccggtgatta aaaattaagg agggtgtaac gacaagttgc
     6781 aggcacaaaa aaaccacccg aaggtggttt cacgacactg cttattactt tgattttatt
     6841 cttatctttc ccatggtacc cggagcggga cttgaacccg cacagcgcga acgccgaggg
     6901 attttaaatc ccttgtgtct accgattcca ccatccgggc tcgggaagaa agtggaggcg
     6961 cgttccggag tcgaaccgga ctagacggat ttgcaatccg ctacataacc gctttgttaa
     7021 cgcgccaaat tcttcaggcc tttcagccag acatccgctt gacgccgatg tcttttaaac
     7081 tggagcggga aacgagactc gaactcgaga ccccgacctt ggcaaggtca tgctctacca
     7141 actgagctat tcccgtattc atcaagcaat cagttaatca cttgatttta ttatcgtctg
     7201 gcaatcagtg ccgccgttcg atgcgttgca ttctacttac ctggcgcgat gagtcaacga
     7261 tatttttcac cacttttgat cgtttgctga aaattacgcc gaaacgatca ctgatcaagc
     7321 aaatctgcac gcgcagcgct caaatattgc aacattgacc acagagtcag taccgcagcc
     7381 acaaagaaaa gtgcaatacc ggcgtactca acccaaatgt tcggacgcca cagcagccag
     7441 gccaacgcca ccatctgagc agtggttttc actttcccaa tccaggagac ggccacgcta
     7501 ctgcgtttac ccaactccgc catccattcg cgtagcgcag aaataataat ttcacgggcg
     7561 atcatcgttg ccgccggtaa tgtcacccac cagctgtgat aatgctcggt taccagcacc
     7621 atggcgatag ccacgagaac tttatctgcc acagggtcaa ggaaagcacc aaaccgggta
     7681 ctctggttcc agcggcgtgc cagaaaacca tcgaaccagt cagtcaccgc cgcgacgcag
     7741 aaaatgagcg cggcggcaaa cggcgaccag gtgacaggca gataaaagac caatacaaag
     7801 aatgggataa ggatgacacg gaacagtgta agcaacgtag ggatattaaa ttgcataatg
     7861 acgggtaact atctgttgtc agtaagatta cccctatgtt gctacagaga catcaatgtt
     7921 tcaacgacca gaagatcttt tctgccagac cttgcgaaat acccggcact tttgcaattt
     7981 cctcgacgct ggcgttacgt aaaccttgca aaccgcccat atatttcaac aacatttgcc
     8041 gacgttttgg cccgacgcct tcaatggttt ccagggaact ggtatttttg accttcgccc
     8101 gttttttacg gtgcccgcca atcgcgtgat catgtgattc atcgcgaata tgctggataa
     8161 catgcagcgc gggtgaatct ggcggcaaac taaatccctc accttccggc tcaaagaaca
     8221 gcgtttccag tccagcctta cgatctgctc ctttggcaac gccaagtagc agcggatgat
     8281 ttttatccca tgagacatcc agttcggcga agacattttt cgcctgcgca agctggcctt
     8341 tgccgccgtc gataaggatc acatccggga tcttactgtc gtcaatggct ttaccataac
     8401 gccgacgcag cacctgattc atcgccgcat aatcatcgcc cggcgtgatg cctgtaatgt
     8461 tatagcgccg atactccgca cgcagcgggc cgttagcatc aaacaccaca caggaagcga
     8521 cggtttgttc gcccatggta tggctgatgt caaagcactc catccgcttc acttccggca
     8581 atttcaacac gctggcaagc gcggtcagtc gctggtgaac ggtagattgc tgcgaaagtt
     8641 tgctggttaa ggccgtcgcc gcattggtgc gcgcgagttt cagataacgc gccctatcgc
     8701 cgcgaggttt ggtttgaaca ttaatcttgc gtcccgccag ttctgaaagg gaatcggcga
     8761 gcagcgtttt atcgctaaga ttaaaatcga gcaggatctc acccggtaag gtgcgcatct
     8821 ggctgccttg taaatagaac tgacctacga aggtttctac cacctcgctc agttccgtac
     8881 cgccaggcac tttcgggaaa tagctgcggc tgccgagcac tttgccctga cgaatgaaca
     8941 atacgtggac acaagccatg cccgcatcga acgccacacc aataacgtca aggtcgtcgc
     9001 cggtattgga aacaaactgt ttttcggtga cgcgtcgcac cgcctgaatt tggtcgcgaa
     9061 tacatgccgc ttcttcaaac tccagattct ggctggcagt ttccatacgg ctaatgagtt
     9121 gcgtaagcac ctgatcatct ttgccagaca aaaacaggcg cacatactcg acctgctgag
     9181 cgtattcttc ttcactcacc agtccttcaa cgcacggtcc cagacagcgc cctatctggt
     9241 attgcagaca cggacgcgag cgattgcgat aaacactatt ttcgcactgg cgaatgggga
     9301 aaatcttttg cagtagcgcc agtgtttcac gtacggcata gccattcggg aacgggccga
     9361 aatattcacc tttggcatgc tttgcaccac gatgcatcgc cagacgcggg tgggtatcgc
     9421 cactcaggaa gataaaagga taagatttat catcgcgtag caaaacgttg taacgcggct
     9481 gatagagttt gatgtagttg tgttccagca acagcgcttc ggtttctgta tgcgtaaccg
     9541 ttacatcaat ttgctggatc tgggcgacca acgcttcggt tttgcgcgaa gcgaggttgc
     9601 tgcggaaata gctggaaagc cgttttttca ggtctttcgc tttgccgaca tagataaccg
     9661 taccaccagc atcgtacatg cgataaacgc ctggctggct ggttacggtt tttaaaaacg
     9721 cttttgcgtc aaactgatca ctcactgact tgataatgtc tccgcattac acagaccatg
     9781 gcgaattgcc aggtgagtca gctcaacatc gccatgaatg tttagtttac tgaacatacg
     9841 atagcggtag ctgttcaccg ttttcggact gagattgagc tgttctgaga tctcattgac
     9901 cttctggccc ttagtgatca tcagcataat ctgcaattca cgttcagaca aactggcaaa
     9961 tgggctttct gttttttctg gttcgatctg gcttaacgcc atttgttgag cgatatcaga
    10021 agcaatgtaa cgctgccctg aatagacaga acgaatcgca ctcacgactt cctgcggagc
    10081 cgcgcctttg ctgaggtagc cagcagcacc tgcctgcatg actttcgctg gtaaagggtt
    10141 ttctgtatgg accgtaagca tgatgatttt aacatcagct gtggaacgcg cgattttacg
    10201 cgtcgcctca agaccgccaa tgcccggcat actcatgtcc attagcacca cgtcaacggc
    10261 atttgtccgg caccacttaa cggcgtcttc accgcacgat gcctctccga cgacttttat
    10321 accctttata tcttccagaa tgcgtcgtat ccctgcgcgc accagttcgt ggtcatcaac
    10381 aagtagaacg ttgatcaaag gaatatctcc agaaataggg ataacgctac tgatagttag
    10441 tcattcgtat attagcggtt tttattgcaa ctttgaaacg ttaaaaatgg tgggtattcg
    10501 atttttctct cgtttttgtc aattcgattg tccacagtta tggtcacgcc cgtcaccaac
    10561 ggcttaccgg atcgtttcac agtaagccct acgatgaaat gtgacaaaaa ttgactttat
    10621 tcagcaaaaa tgaaaatcag cccgctgaat atgtaacatt aattaacccg aacaatacgg
    10681 gcaaaaaaca ttcatgataa aaatatttat tggtcattat attaacgtat tttatagcac
    10741 tgccgatatc acgctcaaaa aacaaccact gctattttta gcaaagctta tggtatactc
    10801 cgccgcctta acatttttca ccgcaaattt tcattgcaac atgacgagga aaataaatga
    10861 gtacgcctga tttttctact gccgagaata atcaagaact ggcaaatgaa gtctcctgcc
    10921 tgaaagcgat gctgacgctg atactgcagg cgatgggaca agctgacgcg ggccgcgtga
    10981 tgttaaagat ggaaaaacag cttgcgctga tcgaagacga aacccaggct gcagtatttt
    11041 ccaaaacggt taagcaaatt aaacaagcct accgtcagta atataaaacc ggctgagagc
    11101 gtgcctttca gccggttatt gacaaaatca tcgctatctc tgctgatgtc ttcccttccg
    11161 ccgtccattt cagaatttct ttttcgcgct tgctgaaatt catctctggc gtcatcacta
    11221 tttcatcatt taaacgcatc agagccatca gactttcgcg caccagtaac tgcattttta
    11281 attgcagttc atcactaaga atgggtattt cgcgcgtgct gcaacgggaa aaggacaaaa
    11341 agcccagcgc ccgatttggc agcattaaat actgagtgac accgcggcgt aaaccatgtg
    11401 cgtgcgcggc ttcccataat ggctgtgctt cgctgaataa gtcatcattc cacattaaat
    11461 ggtcctgact aaagttttca gggttcagca caggatcaat tgcgagaaaa ttttttgcct
    11521 gataataact aacccacgac tcagggtaat tggtgtagaa agccacttta ggtcgcgtga
    11581 atggtaccgg gtggcgaaca cataacgaat agtaatcgta ctccagctgc tgagcctgaa
    11641 gctctatttc atggtagacc tcttctgcgg cctccatcct ctgaaaacgc aacagcatcg
    11701 tgcgacgcca gctgaaaaaa tccgtatcct gcatagtaaa ccgcaacgcc cctgagagtg
    11761 agtatcattt ataatgataa ttcaaactat cacataaata agatttatat ataatttata
    11821 ttattcaggc aatgaattac ttttgcaagc catcgcattc tcttatgtta ttaatgagtt
    11881 atgctgattt gttaagcagt tttatcacgc ttgaaatggc gtcccgcccc gacaggtgaa
    11941 tcgtcggggc tgattttttc ttattattgc agcagaagct tctcgaggaa ctggcgggtg
    12001 cgaggctgct ggggatcggc aaataacgct tttgctgccc cctgctcgac tatccgcccc
    12061 tggtccataa agatcgcccg gtccgcaaca tcccgggcaa agctcatttc gtgtgtcaca
    12121 atcaccatcg tgcgcttttc ctgcgccagc tggcggatgg tgttcaggac ttcgcccacc
    12181 agctctggat ccagcgccga cgttggctcg tcaaacaaaa tcacctcagg acgcattgcc
    12241 agtgcacgcg caatcgcaac acgctgctgt tgacctccag acaaacgacg tggatagctg
    12301 gtttctttac cggccagccc aactttcgcc agcagctcgc gagcgcgcgc cgtggcctct
    12361 tctttcggtt cgcctttgac gatcaccggc ccttcaataa tgttttccag caccgtacga
    12421 tgcggaaaca aattaaagtt ctggaagaca aacccgacgt gctgacgcaa ctggcgaatc
    12481 agcgattttt gctgacttaa tgaacgtgca gtatcaatag tgatatcgcc aacggtgatc
    12541 gttcccgctt ccggttgttc cagcagattt atgctacgta gcaatgtggt tttgccggaa
    12601 ccactcggac cgataattgc caccacttcg ccaggcttta cctcgaggtc gataccgtgc
    12661 agcaccgtct gaccgtggaa ttttttcacc aggttcttaa cttcaatggc actcattttg
    12721 gttctctctc ctggcgatta agttgattct caaaatggtt ctgcaacgtc gataacacag
    12781 tcgccatgat ccagtagatg agcgaagccg ccaggtacat ggtgaacacc tccagcgtgc
    12841 gcgaggtaat caactgcgcc tgacggaaca gctccggcac ctggattgtc gcggccagcg
    12901 acgtatcttt taccagactg ataaagctgt tcgacagcgg cggcaacgcc acccgcgccg
    12961 cctgtggcaa aatcgcgcga cgcatggtct gccacggcgt cataccaata ctggcagcgg
    13021 cttcccactg acctttatca atagaagaaa tggcggcgcg cagcgtttcg gcggcatagg
    13081 cggcagtgtt cagcgacaga ccaatcattg ctgacggaat gggatctaat tcgataccaa
    13141 actgcggcag gccgtaatag atcataaaca gttgtgcgat gagcggcgta ccacgaaaaa
    13201 tagagatata aaaacgcgcc agccagcgca cgggccagat tggcgacagg cgcatcagcg
    13261 cgagaataaa cccgagtagt aagccaaaaa acatgccgcc gatactgagt tgcagggtat
    13321 acccagcccc tttcaacagg aacggcaagg aatcaataac cagttgtata ctttcttgca
    13381 tgagcgttcc cggagttaaa cgtggggatg ataggcgaac agcgcaggcg cgccgccggt
    13441 atgaataaac agaatcggcc cttcatcttt gaagcgtttc tgactgatac cgtcaatcag
    13501 ccccgccatc gcttttccgg tatacacagg atcaagcaga ataccttcca gacgcgccag
    13561 caatttcact gcttccatgc cttcgtcgtt cggcacgccg tagccaggtg caaaatagtc
    13621 atcccagagt aaaatttccg ctgatgcggt cagctccagt tctttcgcaa tcgcctgttg
    13681 taggttaacc actttcggca attgatcggc aacggaacgc gacacggtca cgccaatcag
    13741 ttcgctttca ggcatcaggt gttccagccc aacagccagt ccggcgtgag ttccggcact
    13801 gcccgatgcg actaccaccg acgaaatatt aaccgcccct tcacactgtt gcgcgatttc
    13861 cagcgcactc tccacataac ctagcgcgcc aagagcatta gaaccgccaa ccggaatgac
    13921 atacgggcga aagccttgtg cttcgactcg cgtcgccagc tcttccagtt gggcattggg
    13981 atcggtcagt gcgtcgcaca tttcaatctg ggtattgaac agatccagca acaaacgatt
    14041 gccgttggtt aaatagtttt ctgcggttgt gccaatagga ttttccagca gcgccacgca
    14101 gtgcagaccg agtttcgccg cgactgcggc agtctggcgc acatggttag actggatcgc
    14161 gccggcagta atcagcgtat cggcaccttc gcgcagggca tctgccgcga gaaattccag
    14221 cttacgtaat ttattgccgc ccattgccat gggggtgacc tcatcccgtt tgatgaaaat
    14281 ttcccgtcca agataatcag aaaagcgcgg cagatattcg agcggcgttg gcgcgccgat
    14341 aaactccaac cgtggaaaac gggttaaatt atgcagtggc ataacagcct ccgatgtgtg
    14401 ttgttgtgat tttcttatta tgcacgctga aaacgcgtaa ataaaaaagg cgctagtgaa
    14461 agcgcccttt tttgtcatta tgctgattat ttggtcacat cagcaccaaa ccatttttcg
    14521 gaaagagctt gcagagtgcc gtctttttgc atttccgcaa ttgcatcatt caccgctttc
    14581 agcaggtctt catttccttt acgcagcgcc acgccagact cctgacggga gaatgcttca
    14641 ccggttactg ccagcgtatc gttggttttc ttcaccagat ccagcgccgc cagacgatca
    14701 acgaggatcg catcgatacg ccctacgcgc agatcctgat atttggtcgg gtcatcatca
    14761 taggtacgca catcgacacc ctgaacattc tgccgcagcc actcttcata gttggtgccc
    14821 agaccgacac ccactttttt gcctttcaga tcagcagccg ttttaatggt gccttcgtta
    14881 ccttttttca ccagcgcctg aataccggaa atggtgtaag gggtagagaa atcgtatttt
    14941 ttcttgcgct cgtcagaaat ggtgacctga ttaatcacca catcaatgcg tttagagtcc
    15001 agcgacgcca gcataccgtc ccatttggtc ggttttagtg acgcctcaac gccaagatgt
    15061 tttgccagct gttgggcaaa ttccacttca aaaccggtta atttgccgtc atccccctga
    15121 aaactgaacg gcggataagt gccttccagc cctaccagca gcgtgccgcg ctctttgact
    15181 ttattaagca gaccttcatc tgcaaaactt ttaacgctca tgcccgcaac cagcgccacg
    15241 cccatcacac ccatcaatgc ctgacgtccc agatgtgcta atttcatatt caccccgaat
    15301 gttgttatgt ctgtttgcag tgtagagcca ttgtttgtaa acacaaaaac aactccgcta
    15361 catcttattc ttatttaata tatatcagaa gcaggcaggc tggaggattt ctgcacaagt
    15421 ccggtacagg tttggcgctg atagtgttga tacttccgta atgcgatgcg gtaattgttg
    15481 gtgctggtgg tgggcagcca cggggcgagg atttcatcaa ggaaaccgtc ctgcagctga
    15541 tcgagggaaa tattttgctc atgtagatga ttacccaaac ggcgcagacg aacgacatat
    15601 tcacgcaccg tacctgggct catttcagtt tgctcaaaaa gaaatttctt aaagccgata
    15661 atatcgaaaa aatcactctg cgttttgcaa tgtaaatcac cgcaaaatcg gcacaatgcc
    15721 gcccatgatt gttgttccgt ttgccagcct ttttcatcaa gcagcgtgtc caggcgggaa
    15781 atctcgattt tattcactat tttgccgtcg cgaactaagg taatgcgatc gagtaattta
    15841 cggcaatgcg cgcaatgggt ctggctgtgt ttaaagtctt taagatagcg gcttaatggc
    15901 cgtcttttca ggtgctgcac catcattaag aactcctggt agtcaaagtt aaagtgcggc
    15961 atttactgac gttataactt acccagttta gtgcgtaacc gtttaatggc ctggctgtgt
    16021 aactgactga cccgcgattc cccgacctcc agcaccgcgc caatctcttt gagattcagc
    16081 tcttcctggt aatagagggt taataccagt ttttcgcgct ccggcaacgt ttcgatggct
    16141 tccatcaccc gctggcgcag attactgtcc agcagttgtt gtagcgggtt ttctcgctga
    16201 tgatcatcag taaccagttc gatgctatcg ccgtgctctt cacgccactc atcgtaggag
    16261 aagagctggc tgttattggt gtcgagcagc atttgacgat aatcggcaat atcgatccct
    16321 aaacgttccg ctacctcagt ttccgtggcg ttgcggccaa gttcctgctc cagttgccct
    16381 attgcctgtg ccacttcacg cgcgttgcgt cgcacgctgc gcggcaccca gtcacggctg
    16441 cgaagttcat ccagcatagc gccacggata cgctgcactg cgtaagttgt aaatgccgtt
    16501 ccttgtgggg cgtcatagcg ttcgacagca ttaagtaacc caatgccgcc cgcctgtagc
    16561 agatcgtcaa gttccacgct cgcgggcagt cgaacctgca ggcgcaatgc ttcgtgacgc
    16621 accagcggga cataacgctg ccacagcgag tgtttatcca ttacaccttc agcggtatag
    16681 agtgaattca cgataaacag ccctgcgtta tatgagttat cggcatgatt atccgtttct
    16741 gcagggtttt taatcggacg attagtgggt gaaatgaggg gttatttggg ggttacgcaa
    16801 aaagccgaat gtaaaaaacc ccgtcggtgg cggggtttga gaggcattca gacgattaac
    16861 cctgcagcag agacagaacc tgctgcggta cctggttggc ttttgccagc acggagttac
    16921 cggcctgcag gataatctgc gctttcgaca tgttggacac ttcggtcgca tagtcggcgt
    16981 cctgaatacg ggactgcgct tcagacaggt tggtagtggt gttgttcagg ttggtgactg
    17041 cggaatccaa acggttttgc accgcaccca gggaggagcg gaatttgtcg atggagctga
    17101 tggcttcatc cagagctttc aggggatcgg ctgtcgtagc tgcttttgtt tcagcatctg
    17161 tcgtcaattt accatcagca tctttataaa ctgccttacc gctgccgtta gtcactgaac
    17221 catcatcctg taggtaaaaa tctacagtat cagatgtggt caacgcctcg ttgttatcaa
    17281 cataaaccga atcaacacct tttgttacag cgccagttgt gccgttgacg ctataacctg
    17341 cagtagtgaa atcaacattg tttcccgagg ttgatacagc tttatcgaaa gctgcctgat
    17401 caacttttgc acctgttact gaataagtaa tagtgtccgg cgtagtacta ttaccggtaa
    17461 aggagataga ggtaccgcca atgtcaatgg ttgtcgcttt ggtattattg gctgcaagtg
    17521 tggacagact agcctcagtc aaagaagcac cagagccgtt tttcgttaag cgcccagttg
    17581 tatcaatgta gagtttatct ccattgcttg ccgtaatttt accatcactg ctgatctgaa
    17641 catcctgtgc agaaccatcg atagtaatag tgcccttagc ggtatcacca actcccgggg
    17701 tcaaatattt ctgaacatcg gcagctgaag ccgttgtggt atcaaaagag tatgacttac
    17761 ttgcactgtc atacttgtaa ttcgttgcac tagccgccgt tccgaagccg ttattaatgg
    17821 ttgcctgaac agtatcacca tcactaactc cagccagcaa atcagacgct ttagcagcat
    17881 cgtaacccgc actcacagta tatttgttgc cgaccactgt tgcattagca gctaccaagt
    17941 cagctttaga tgcagcagta ttagccacag ccccgccacc attcacatta aacccattca
    18001 gccccagcgt atcagagtca attttcttca gatcaatagt gatagtctgg ccgtcattcg
    18061 caccaacctg aattttcatc gaaccgtctt ttgccagtac gttcacgccg ttgaactggg
    18121 tctgaccgga tacgcggtca atttcgtcga gacgggattt gatttcgtcc tgaatggagt
    18181 ccagatccga atcagagtta gtcccggtag aagcctgaac cgtcagctca cggatacgct
    18241 gtaagttgtt gttgatttcg gacagcgcgc cttcagtggt ctgtgcaaca gaaataccgt
    18301 cgttggcgtt acgtgcagcc tgagtcaggc ctttaatgtt agaagtaaaa cggttagcaa
    18361 tcgcctgacc cgcggcgtca tccttcgcgc tgttaatacg caagccagaa gacagacgct
    18421 cgatagaact cgacagcgca gactggttct tgttgatatt attttgagtg atcagcgaga
    18481 ggctgttggt attaatgact tgtgccatga ttcgttatcc tatattgcaa gtcgttgatt
    18541 acgttttggg tttccacccg tcggctcaat cgccgtcaac cctgttatcg tctgtcgtaa
    18601 aacaaccttt agaatttttt tcacaaacag ccattttttg ttagtcgacg aaatactctt
    18661 ttctctgccc cttattcccg ctattaaaaa aaacaattaa acgtaaactt tgcgcaattc
    18721 aggccgataa ccccggtatt cgttttacgt gtcgaaagat aaaaggaaat cgcatggcaa
    18781 gtatttcatc gctgggagtc gggtcaggtc tggatttaag ttcaatcctt gatagcctca
    18841 ccgccgcgca aaaagcgacg ctaaccccta tttcaaatca gcaatcgtcg tttaccgcta
    18901 aacttagcgc ctacggtacg ctgaaaagcg cgctgacgac tttccagacc gccaatactg
    18961 cattgtctaa agccgatctt ttttccgcca ccagcaccac cagcagcacc accgcgttca
    19021 gtgccaccac cgcgggtaac gccatcgccg ggaaatacac catcagcgtc acccatctgg
    19081 cgcaggcgca aaccctgacc acgcgcacca ccagagatga tacgaaaacg gcgatcgcca
    19141 ccagcgacag caaactcacc attcaacaag gcggcgacaa agatccgatt accattgata
    19201 tcagcgcggc taactcatcg ttaagcggga tccgtgatgc catcaacaac gcaaaagcgg
    19261 gcgtaagtgc gagcatcatt aacgtgggta acggtgaata tcgtctgtca gtcacatcaa
    19321 atgacaccgg ccttgataat gcgatgacac tctcggtcag cggtgatgat gcgctacaaa
    19381 gttttatggg ctatgacgcc agtgccagca gaaacggtat ggaggtctcg gtcgccgccc
    19441 agaatgcgca gctgacggtc aacaacgtcg ccatcgagaa cagcagcaac actatcagcg
    19501 acgcgctgga aaacatcacc ctgaacctga acgatgtcac cacgggcaac cagacgctaa
    19561 ccatcactca ggacacctcc aaagcgcaaa cggcgattaa agactgggtg aatgcctata
    19621 actcgctaat agataccttc agcagcctga ccaaatacac cgccgtagat gcgggagctg
    19681 atagccagag ttctagcaat ggcgcactgc tcggcgactc cacgctgcgg acgattcaga
    19741 cgcagttgaa gtcgatgctg agtaataccg tcagttcttc cagctataaa acgctggcgc
    19801 agattggtat cacgaccgat cccagcgatg gcaaactgga actggatgcc gacaaactca
    19861 ccgctgcact gaaaaaagat gccagcggcg taggtgcatt gattgttggc gatggtaaaa
    19921 aaaccggcat cacaaccacc atcggcagca acctgaccag ttggctttcg acaacgggca
    19981 ttattaaagc cgctaccgat ggcgttagta aaaccctgaa taagttaact aaagactaca
    20041 acgccgccag cgatcgcatt gatgcgcagg tcgcgcgcta caaagaacaa tttacccaac
    20101 tggacgtttt aatgacctcg ttaaacagca ccagcagcta cttaacgcag cagttcgaaa
    20161 acaacagtaa ttccaagtaa tcattattca tcgggagaca ggtcatgtac gcggcaaaag
    20221 gcacccaggc ctatgcgcaa attggcgtcg aaagcaccgt aatgagcgcc agccaacagc
    20281 agttggtcac catgctattt gatggagtgc tgagcgcact ggttagagcg cgactgttta
    20341 tgcaggacaa caatcagcaa ggcaaaggcg tctctttgtc aaaagcgatc aacatcattg
    20401 agaacggact gcgggtgagt cttgatgaag agagcaaaga cgaattaacc caaaacttaa
    20461 ttgctcttta tagctatatg gtcaggcgtt tgctgcaagc caatttacgc aacgatgtct
    20521 ccgcagtcga agaggtggaa gcattaatgc gtaatattgc cgatgcctgg aaagagtctt
    20581 tactctcccc ttcttcgatt caggacccag tctgatgaac aatgcaccgc atttgtattt
    20641 cgcctggcaa caactcgtcg aaaaaagcca gctcatgtta cgcctggcaa cggaagaaca
    20701 atgggacgaa ctcatcgcta gcgaaatggc gtatgtgaat gcggtgcagg agatcgcaca
    20761 cctgactgaa gaggttgccc cgtccactac gatgcaggag cagctccgcc cgatgctgca
    20821 cctgattctc gacaacgaaa gcaaggtaaa gcagttatta cagattcgga tggatgaact
    20881 ggcgaaactg gtcggtcagt catcggtgca aaaatcggtg ttaagtgcct atggcgatca
    20941 gggcggcttt gtgctggctc cgcaggataa cctcttttga atcttaatga gccgatggct
    21001 cgcgaataat ccgattacgg ctacgcttct aatattcccc ttgaatggag tcgaagaatg
    21061 cgtaatccca cgctgttaca atgttttcac tggtattacc cggaaggcgg taagctctgg
    21121 cctgaactgg ccgagcgcgc agacggtttt aatgatattg gtatcaatat ggtctggttg
    21181 ccgcccgcct ataaaggcgc atcgggcggc tattcggtcg gctacgactc ctatgattta
    21241 tttgatctag gtgagtttga tcagaaaggc agcatcccta ctaaatatgg tgataaagca
    21301 caactgctgg ccgccattga cgccctgaaa cgtaatgaca ttgcggtgct gctggacgtg
    21361 gtagtcaacc acaaaatggg cgcggatgaa aaagaagcta ttcgcgtgca gcgtgtaaat
    21421 gctgatgacc gtacgcaaat tgacgaagaa atcattgagt gtgaaggctg gacgcgttac
    21481 accttccccg ctcgtgccgg gcaatactcg cagtttatct gggatttcaa atgttttagc
    21541 ggtatcgacc atatcgaaca ccctgatgaa gatggcattt ttaaaattgt taacgactat
    21601 accggagaag gctggaacga tcaggttgat gatgaactcg gtaatttcga ttatctgatg
    21661 ggtgagaata tcgattttcg caatcatgcc gtgacggaag agattaaata ctgggcgcgc
    21721 tgggtgatgg aacaaacgca atgcgacggc tttcgtcttg atgcggtcaa acatattccg
    21781 gcatggtttt ataaagagtg gatcgaacac gtacaggaag ttgcgccaaa gccgctgttt
    21841 attgtggctg aatactggtc gcatgaagtc gataaactgc aaacgtatat tgatcaggtg
    21901 gaaggcaaaa ccatgctgtt tgatgcgccg ctgcagatga aattccatga agcatcgcgc
    21961 atgggtcgcg actacgacat gacgcagatc ttcactggga cgctggtgga agccgatcct
    22021 tttcacgccg taaccctcgt tgccaaccac gacacccaac cgttgcaagc cctcgaagca
    22081 ccggtcgaac cgtggtttaa accgctggcg tatgccttaa ttttgttgcg ggaaaatggc
    22141 gttccttcgg tgttctatcc ggacctctac ggtgcacatt acgaagatgt cggtggtgac
    22201 gggcaaacct atccgataga tatgccaata atcgaacagc ttgatgagtt aattctcgcc
    22261 cgtcagcgtt tcgcccacgg tgtacagaca ttatttttcg accatccgaa ctgcattgcc
    22321 tttagccgca gtggcaccga cgaatatccc ggctgcgtgg tggtcatgtc gaacggagat
    22381 gatggcgaaa aaaccattca tctgggagag aattacggca ataaaacctg gcgtgatttt
    22441 ctggggaacc ggcaagagag cgtagtgaca gacgaaaacg gcgaagcgac cttcttttgc
    22501 aacggcggca gcgtcagcgt gtgggttatc gaagaggtga tttaaatcca tccccggcgg
    22561 caagccgggg agatttcatt acggcagtgg cgtcggtaac ggcgctttat ccagcgcggc
    22621 ggcacactct gccgtcgggc gatccacacg ctccatcgtc ataccatcgt actcaatggt
    22681 gttaccgtca cgctcaacct cgtacagctc acgttttacg gtaacgttag tgagatcatc
    22741 agacatcaat gtcaacttac ccggcacggc aatcccccgc tgccactgac ggcaatccaa
    22801 ggtatcgccc tctttagtga caatcaggct gcctatcgct tccggactga ccagcgcacg
    22861 ttgcggccct ttggtctgcc agtagcccgc cagccagtcc ggcgcggggg ttttcacgac
    22921 attgttatag ttttcgactt cggcacagcc cgccagcaac atgagcgcgc ctgcaattgc
    22981 gagttttttc atcattcgtc ctgcatgaga agaaaagagg attgtggcat taaagctctg
    23041 atgtcgccag cctttccagg ttcagagaga aatttgatct aactattatt tttgtgaatt
    23101 attaccccga aaataaattt gcatggtgta tgattcgcgg ctcttgtttc aaaccttctg
    23161 agttcagagg ctacattcat gtcatggcag caattcaaac acgcctggtt gattaaattc
    23221 tgggcaccca tccctgcggt catcgcggcg ggtattctct ccacttacta ttttggcatt
    23281 actggcacct tttgggctgt cacgggtgaa tttacccgtt ggggcggtca gctcctgcag
    23341 ctgtttggcg tccatgctga agagtggggt tattttaaaa ttatccatct ggaaggatcg
    23401 ccattaaccc gcatcgacgg aatgatgatc ctcggtatgt ttggcggctg ctttgctgct
    23461 gcgctgtggg ccaacaatgt caaactacgc atgccacgca gccgtatccg cattatgcag
    23521 gccatcattg gcggcattat cgccggtttt ggcgcgcgtc tggcaatggg ctgtaacctg
    23581 gcggcgttct ttaccggcat tcctcagttc tcgctgcatg cctggttctt tgccatcgcc
    23641 actgccattg gttcatggtt tggcgcgcgc tttaccctgc tgcccatctt ccgtattccc
    23701 gtgaaaatgc agaaagtttc tgccgcctca ccgctgacgc aaaaaccgga tcaggcgcgg
    23761 cgtcgttttc gtctcgggat gctggtcttt ttcggcatgc tgggctgggc gctgctcacg
    23821 gcgatgaacc aaccaaaact ggggctggca atgctgtttg gcgtcggctt tggtttactg
    23881 attgaacgtg cgcaaatctg ctttacttcg gcgttccgcg atatgtggat caccggacgt
    23941 acccatatgg cgaaagcaat cattatcggt atggcggtga gtgccatcgg gatcttcagt
    24001 tacgtacagt taggcgttga acctaaaatc atgtgggcgg gaccaaatgc ggtaattggt
    24061 ggtttacttt ttggttttgg catcgtgctg gcaggcggct gcgaaaccgg ctggatgtac
    24121 cgcgcggtag aaggccaggt gcactactgg tgggtcggtc tgggcaacgt gatcggctca
    24181 acgattctgg cgtattactg ggatgatttc gctccggcgc tggccaccga ctgggacaaa
    24241 atcaacctgc tgaaaacttt tggtccgatg ggaggcctgc tggtgacata tttgctgctg
    24301 tttgctgcgc tgatgttgat tatcggctgg gaaaaacgct tcttccgccg tgcggcaccg
    24361 cagactgcta aggagatcgc atgaaaaata tcgttcctga ttaccgcctt gatatggtgg
    24421 gtgaaccctg cccttatccg gcagtcgcaa cccttgaggc gatgccgcaa ttaaaaaaag
    24481 gtgagatcct ggaagtggtg agcgactgtc cgcagtcgat caataatatt ccactggatg
    24541 cgcgtaatca cggctacact gtgctggata ttcagcaaga cgggccgacc attcgttatt
    24601 taattcagaa gtaatccctc attcccgctg tggcccacag cgggaacatc gttacctctg
    24661 acacatagat tttacccgcc ccctctccta ttcttttcag tatgatctca ggaggttgct
    24721 atgtgtggac gctgtgccca atcccaaacg cgtgaggatt accttgcgct tctcgcggaa
    24781 gatattgaac gcgatattcc ctacgatccc gaacccattg gcagatacaa tgtcgcgccg
    24841 ggaaccaaag ttcttctgct cagtgaacgt gatgaacacc ttcatctgga tccggttttc
    24901 tggggatatg ctcccggatg gtgggataaa ccgccgctga ttaacgcccg cgtagaaact
    24961 gcctcaacca gtcgcatgtt taaaccgctc tggcaacatg gtcgggcaat ctgttttgcc
    25021 gatggctggt ttgagtggaa aaaagaaggc gacaaaaaaa gccttatttt atctatcgcg
    25081 ctgacggaca acctgttttt atggccgcga taggtagcat accgtttgag cgtggtgacg
    25141 aagccgaagg atttttgatc gtcactgcgg cggcagatca aggtctggta gatattcatg
    25201 accgccgccc gctggttctg tcgccagaag ctgcacggga atggatgcgg caagacattg
    25261 gtggtaaaga agcctcagaa atcgctacca gaagctgtgt gccagcaaac cagttcacct
    25321 ggcaccctgt gtcgcgcacg gtgggtaatg tcaaaaacca gggcgcggag ttaattcaac
    25381 ctgtttgttg acatcctcca cgccctgaat gacgaggccc cctgctacgt tcaggctgtc
    25441 gcctgaatca tttcggtggg ttcctgcttc aacgagcggc ctgactgcac catctctcca
    25501 caggcaagaa cggcgtgccc cgccgctaaa atgttacgag cgccatttac atcggcgttc
    25561 gctgtatatc cacatacctg gcatctgaat ttactttgtg acaggcgatt ttctttcgct
    25621 gtatgaccac agcacgcgca acgctggctt gtgtacgctg gcggaacagc aagcacctga
    25681 ccgccacgcc agagctgctt gtactcaagc tggcggcgca tttcatacca gccctgatcc
    25741 agtatcgaac ggtttaaacc tgattttgcc cggacattgc gccccggctg gctgaccgta
    25801 cccgctgctg actttgacat gttgctgacc ttcaaatcct caatgactat cattacgtgg
    25861 tttttgctga cggtcgttgt gactttgtga aggtagtccc tgcggatatt tgcgatacag
    25921 gaatgcagtc gctgtatttt gcgtttctgc ttctgccagt tgttgctgaa tttgaccttg
    25981 cggcttaact gtcgctgaag tctcgccagc ttcttctggt ttttctgaaa actgtttaca
    26041 ggctcaaaga ctgtgccatc tgacagcgag gcaagtttag ccacgccagc atccagcccg
    26101 accattgatg ctgaagggtg aaccggagtt gatacttcac tttctgcctg aatactgatg
    26161 taccacttac cgcaggactg gctgacagtg acatttttca caacacccgt gacctgacgg
    26221 ctgttccggt agcgcatcca gccaagtttt ggcagaaaaa tacggctgtt ttcctgatcg
    26281 agcttaacgc cctgcggata gcggaatgca tcattctgtc accgcttttt gaatcagggg
    26341 aacgccgacc gcttctggaa aaagtttttg caggcctgct cagggtcttt cagtgactgt
    26401 tgcaatggct gtgacggagc atctttaatc cattgcgttt cagtggcatt tttccactca
    26461 accaatcagg aagccatttt cgcgtaaggg atgtctttgt taccggcctt atgattctca
    26521 ttctgacgtg ccagagtacg actgaaaacg aaacgacatg cgcctgcgaa gcgcctcatt
    26581 tcacgcgcct gttgaccacc gggtcttaac cggaatttga atacctgtag tcgtttcaca
    26641 gggcaaatta tactttggtc tgtgaaaaaa actgatatcc gacgcggaag gcactgtgtt
    26701 ttcctgatgc atgctcaccg ggtctttgtt accaagtacc gacgccagat ttttgaccat
    26761 gacgcgacag aaaaactacg tacttacttt tcaaatgtat gtgctgattt tgaagctgaa
    26821 ctggttgaaa tggatggcga accagagcac gtccatctgt taattaacta tccccccaaa
    26881 ctggcgatat ccagtctggt aaacaaacag cctcaaaggc gtatcgggta ggttactgcg
    26941 acgggatcgg ccagatattg ctgccaggta ttaccgcaaa gatgttttgt ggcgtcccgg
    27001 ctattttgcc agtagctgcg gaggtgcgcc aatatccgtc atccgccaat acattgaaca
    27061 acagcaaaca cctggtcagg tgaaaaaacg agccttatat ccccgccctg aaggacgggc
    27121 ttttacggtg cactggataa gcctggagat tatctacacc tgcatggaca tcattgtact
    27181 atgccccgta catgccccat cacatcctca acatcaaagt attagagcct tgcaaaccat
    27241 tatgcgtctc agtttgtctc atctatctta cgcaaaaaaa atagaactaa tatcaagcgg
    27301 cgctatattg cgtattaaat aaaaccacct caattacatt aataaatcta aataaatatc
    27361 atgcaaataa tttccatggt tatttttagg ctatttaaat taaaaaaata aattatgagt
    27421 caatcataaa taacaacctt taatataatt tgactaccaa atttatattc gttcacaaaa
    27481 tagtcatgca ttgtgtgcaa tagaaacagt tcagataaaa atagggatct acttcacaat
    27541 ttaaatgaga aactaaaact tacatcttga aataatctca ttgattggat gaatatttgt
    27601 agtgcagtga catcattttt aaataatagt tcaaataaaa gggctcacga tgaaaaaatt
    27661 aacagtggca atttctgctg tagctgcatc agtactgatg gcgatgtctg ctcaggcagc
    27721 agagatttac aataaagaca gtaacaagct ggatctgtac ggaaaagtta atgctaagca
    27781 ctacttctct tctaacgatg cagatgatgg cgacacaact tatgttcgtc tgggcttcaa
    27841 aggcgaaact caaatcaacg atcaactgac tggtttcggt cagtgggaat atgaattcaa
    27901 aggcaaccgt gctgaatctc aaggttcttc caaagataaa acccgtcttg ccttcgctgg
    27961 cctgaaattc ggtgactacg gctccatcga ttatggccgt aactacggtg tagcatacga
    28021 tatcggtgca tggaccgacg ttctgccaga attcggtggc gatacctgga cccaaacaga
    28081 tgtgttcatg actggtcgca ccacaggtgt tgcaacctat cgtaacaacg acttctttgg
    28141 tctggttgat ggtctgaact ttgctgctca gtatcagggt aaaaatgacc gcactgacgt
    28201 aactgaagct aacggtgatg gtttcggttt ctccactact tatgagtatg aaggattcgg
    28261 tgtaggtgca acctatgcta aatctgaccg cactaatgat caggttatct acggtaacaa
    28321 cagcctgaat gcatctggtc aaaatgctga agtatgggca gctggtctga aatatgatgc
    28381 gaacaacatc tatctggcta ccacctattc tgaaacccag aacatgactg tttttggtaa
    28441 taaccatatt gccaacaaag cacaaaactt cgaagtagtt gcacaatatc agttcgactt
    28501 cggtctgcgt ccgtccgttg cttacctgca atctaaagga aaagacttgg gtgcgtgggg
    28561 tgatcaggac ctgattgaat atattgatgt aggtgcaacc tattacttca acaaaaatat
    28621 gtccactttt gttgattaca aaatcaacct gattgataag agcgatttca cgaaagcatc
    28681 tggcgttgct accgatgata tcgttgctgt aggattggtt taccagttct aatctgatta
    28741 ctcaaagata tgttgtggga ggctttgcct ccccaacata taagtggcgc cctcaagcca
    28801 ctttccttag aagcactatc ttgcttctga ctatataaat taccctttat tttctgggac
    28861 gtttcaacgc cccattttta ataatactca gtgaacaatt aacgtatcaa ttagatttaa
    28921 taacaacgat atccgtacct aacctgatat ctaatgtcat aagcatccct ttaattatcc
    28981 cctcaacctc ttgtaaattt cccactcttg agctccacat agtccaaacg ccggatgcaa
    29041 aaaaaatcac acgtgcatct ctgaaattgt gatggaggaa gtgagtatca attatatgtg
    29101 cttataaacg ttcccgattg gcacagaagt caccggattg ttcaggctcc aataggttga
    29161 gtatgtcgta tttattttcc aaaataaaaa tcgatgctgt tgctccttaa taaaatactt
    29221 ttactctctg gacgcttttc ttagctctct tggtgttatc ttaaaacgat tatggaacct
    29281 ttcagtaaaa cgagaaggac acttgtaacc attttccctg gcaatctcgc tgatgggttt
    29341 tactgtcgtt tgtatagcag acaacgcatt atttaacctc acgtcgtcca atatactttg
    29401 aaaacttatt ccctcgct
//
LOCUS       DAJTYG010000045        29085 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTYG010000045 DAJTYG010000000
VERSION     DAJTYG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.045"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            74..1351
                     /gene="waaA"
                     /locus_tag="OO568_002126"
     CDS             74..1351
                     /gene="waaA"
                     /locus_tag="OO568_002126"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6641474.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1359..1838
                     /gene="coaD"
                     /locus_tag="OO568_002127"
     CDS             1359..1838
                     /gene="coaD"
                     /locus_tag="OO568_002127"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6641475.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1877..2686)
                     /gene="mutM"
                     /locus_tag="OO568_002128"
     CDS             complement(1877..2686)
                     /gene="mutM"
                     /locus_tag="OO568_002128"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6641476.1"
                     /translation="MPELPEVETSRRGIEPNLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2784..2951)
                     /gene="rpmG"
                     /locus_tag="OO568_002129"
     CDS             complement(2784..2951)
                     /gene="rpmG"
                     /locus_tag="OO568_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6641477.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2972..3208)
                     /gene="rpmB"
                     /locus_tag="OO568_002130"
     CDS             complement(2972..3208)
                     /gene="rpmB"
                     /locus_tag="OO568_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6641478.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3425..4093)
                     /gene="radC"
                     /locus_tag="OO568_002131"
                     /pseudo
     CDS             complement(3425..4093)
                     /gene="radC"
                     /locus_tag="OO568_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            4265..5485
                     /gene="coaBC"
                     /locus_tag="OO568_002132"
     CDS             4265..5485
                     /gene="coaBC"
                     /locus_tag="OO568_002132"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6641479.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5463..5921
                     /gene="dut"
                     /locus_tag="OO568_002133"
     CDS             5463..5921
                     /gene="dut"
                     /locus_tag="OO568_002133"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6641480.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYTTSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6028..6624
                     /gene="slmA"
                     /locus_tag="OO568_002134"
     CDS             6028..6624
                     /gene="slmA"
                     /locus_tag="OO568_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6641481.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6661..7302)
                     /gene="pyrE"
                     /locus_tag="OO568_002135"
     CDS             complement(6661..7302)
                     /gene="pyrE"
                     /locus_tag="OO568_002135"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6641482.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7368..8084)
                     /gene="rph"
                     /locus_tag="OO568_002136"
     CDS             complement(7368..8084)
                     /gene="rph"
                     /locus_tag="OO568_002136"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6641483.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8211..9074
                     /locus_tag="OO568_002137"
     CDS             8211..9074
                     /locus_tag="OO568_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6641484.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9295..10119
                     /gene="dinD"
                     /locus_tag="OO568_002138"
     CDS             9295..10119
                     /gene="dinD"
                     /locus_tag="OO568_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6641485.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            10410..11027
                     /locus_tag="OO568_002139"
     CDS             10410..11027
                     /locus_tag="OO568_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6641486.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11024..12706)
                     /gene="ligB"
                     /locus_tag="OO568_002140"
     CDS             complement(11024..12706)
                     /gene="ligB"
                     /locus_tag="OO568_002140"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6641487.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPSRAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12964..13587
                     /gene="gmk"
                     /locus_tag="OO568_002141"
     CDS             12964..13587
                     /gene="gmk"
                     /locus_tag="OO568_002141"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6641488.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13642..13917
                     /gene="rpoZ"
                     /locus_tag="OO568_002142"
     CDS             13642..13917
                     /gene="rpoZ"
                     /locus_tag="OO568_002142"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6641489.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            13936..16044
                     /gene="spoT"
                     /locus_tag="OO568_002143"
     CDS             13936..16044
                     /gene="spoT"
                     /locus_tag="OO568_002143"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6641490.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16051..16740
                     /gene="trmH"
                     /locus_tag="OO568_002144"
     CDS             16051..16740
                     /gene="trmH"
                     /locus_tag="OO568_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6641491.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16746..18827
                     /gene="recG"
                     /locus_tag="OO568_002145"
     CDS             16746..18827
                     /gene="recG"
                     /locus_tag="OO568_002145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6641492.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18861..20066)
                     /gene="gltS"
                     /locus_tag="OO568_002146"
     CDS             complement(18861..20066)
                     /gene="gltS"
                     /locus_tag="OO568_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6641493.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20346..21737
                     /gene="xanP"
                     /locus_tag="OO568_002147"
     CDS             20346..21737
                     /gene="xanP"
                     /locus_tag="OO568_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6641494.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSDFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21858..23567
                     /locus_tag="OO568_002148"
     CDS             21858..23567
                     /locus_tag="OO568_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6641495.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     QLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23620..25938)
                     /gene="yicI"
                     /locus_tag="OO568_002149"
     CDS             complement(23620..25938)
                     /gene="yicI"
                     /locus_tag="OO568_002149"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6641496.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEYFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25948..27331)
                     /locus_tag="OO568_002150"
                     /pseudo
     CDS             complement(25948..27331)
                     /locus_tag="OO568_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27624..27718
                     /locus_tag="OO568_002151"
     tRNA            27624..27718
                     /locus_tag="OO568_002151"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27658..27660,aa:Sec,seq:tca)
     gene            28018..>29085
                     /locus_tag="OO568_002152"
     CDS             28018..>29085
                     /locus_tag="OO568_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6641497.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAH"
BASE COUNT         7185 a         7793 c         7309 g         6798 t
ORIGIN      
        1 acgggaaaag taatggtaaa gccacagcta aatacataga atccccagca catccataag
       61 tcagctattt actatgctcg aattgcttta caccgccctt ctctacctta ttcagccgct
      121 gatctggata cggctctggg tgcgcggacg taaggctccg gcctatcgaa aacgctgggg
      181 tgaacgttac ggtttttacc gccatccgct aaaaccaggc ggcattatgc tgcactccgt
      241 ctccgtcggt gaaactctgg cggcgatccc gttggtgcgc gcgctgcgtc atcgttatcc
      301 tgatttaccg attaccgtta caaccatgac gccaaccggt tcggagcgcg tacaatcggc
      361 tttcgggaag gatgttcagc acgtttatct gccgtatgac ctgcccgatg cgctcaaccg
      421 tttcctgaat aaagtcgacc ccaaactggt gttgattatg gaaaccgaac tatggcctaa
      481 cctgattgcg gcgctacata aacgtaaaat tccgctggtg atcgctaacg cgcgactctc
      541 tgcccgctcg gccgcaggtt atgccaaact gggtaaattc gtccgccgct tgctgcgtcg
      601 tattacgctg attgctgcgc aaaatgaaga agatggtgca cgttttgtgg cgctgggcgc
      661 aaaaaataac caggtaaccg ttaccggtag cctgaaattc gatatttctg taactccgca
      721 gttggctgct aaagccgtga cgctacgccg ccaatgggca ccacaccgcc cggtatggat
      781 tgccaccagc actcacgaag gtgaagagag cgtggtcatt gccgcacatc aggcattgtt
      841 acagcaattc ccgaatttat tgctcatcct ggtaccccgt catccagaac gcttcccgga
      901 tgcgattaac cttgtccgcc aggctggact aagctatatc acacgctctt caggggaagt
      961 cccctccacc agcacgcagg ttgtggttgg cgatacgatg ggcgagttga tgttactgta
     1021 cggcattgcc gatctcgcct ttgttggcgg ttcactggtt gaacgtggtg ggcataatcc
     1081 gctggaagct gccgcacacg ctattccggt attgatgggg ccgcatactt ttaactttaa
     1141 agacatttgc gcgcggctgg agcaggcaag cgggcttatt accgttaccg atgccactac
     1201 gcttgcaaaa gaggtttcct ctttactcac cgacgccgtt taccgtagtt tctatggccg
     1261 tcatgccgtt gaagtactgt atcaaaacca gggcgcgcta cagcgtctgc ttcaactgct
     1321 ggaaccttac ctgccaccga aaacgcattg aggttgttat gcaaaaacgg gcgatttatc
     1381 cgggtacttt cgatcccatt accaatggtc atatcgatat cgtgacgcgc gccacgcaga
     1441 tgttcgatca cgttattctg gcgattgccg ccagccccag taaaaaaccg atgtttaccc
     1501 tggaagagcg tgtggcactg gcacagcagg caaccgcgca tctggggaac gtggaagtgg
     1561 tcgggtttag tgatttgatg gcgaacttcg cccgtaatca acacgctacg gtgctgattc
     1621 gtggcctgcg tgcggtggcg gattttgaat atgaaatgca gctggcgcat atgaatcgcc
     1681 acttaatgcc ggaactggaa agtgtgtttc tgatgccgtc gaaagagtgg tcgtttatct
     1741 cttcatcgtt ggtgaaagag gtggcgcgcc atcagggcga tgtcacccat ttcctgccgg
     1801 agaatgtcca tcaggcgctg atggcaaaat tagcgtagcg tttatgccgg atggtatgcc
     1861 atccggctcg catgaattac ttctggcact gccgacaata aaacgttgcc cgctgcgcat
     1921 gtttagtcgc cacaatcggc gtaccgcaca cccgacacgg ctcacctttt cgcccgtaaa
     1981 cctgcaattc ctgcgcgaaa tagcccggtt taccatcact ttgcagaaaa tctttcagcg
     2041 ttgtaccacc ctgctcaatc gaacgcagca acaccgcttt aatcacccga gctaacaatt
     2101 cacactctgc cagcgacagt gatgacgcca gccgatccgg atggatcccc gccgcaaaca
     2161 gtgattcgct ggcatagata ttccctaccc ctaccaccag cttgttatcc atcagccacg
     2221 gtttaatcgc cgtttttttc ttcgcgcact tctgatgcag atactcacca ttgaaatcgt
     2281 cgctaagcgg ctccggtcca agatgggcca gcacattatg cccttccagc tctttggtcc
     2341 acagccaggc accaaagcgg cgcggatcgg tgtagcgcag cactttaccg ttgctcatca
     2401 ccaaatccac atggtcatgc ttttcagggg gaagttcttc tggaaggatg cgcaggctgc
     2461 cagacatccc caaatgaatg ataatccagc cctcaggcag ctccagcagc agatatttag
     2521 cccgccgctg cacgctaagc actggttgat cgcttaatcg gtagatctct tctgaaaccg
     2581 gccagcgcaa gcgtccgttg cgcaccaccg catgaagaat ggttgcacca acgagattcg
     2641 gttctatgcc gcgacggctg gtttcaactt cgggtaattc aggcatagca tctccaggaa
     2701 tgaacagatg caatcaatat gggggcaagc agataacaaa aaaccccgcc ggagcgaggt
     2761 ttttcgttac atcaaagcga gaattatttg attttcgctt ctttgtagat cacgtgctga
     2821 cgaacaactg gatcgaattt tttcagttcc agtttttccg gcttagtacg tttgttcttc
     2881 gtagtggtat agaagtgacc agtaccagca gaagaaacca gcttgatttt ctcacgaata
     2941 cctttagcca tgatttattt cctctaagta cttagtactt ttcgccacgg gcacgcagtt
     3001 cagccagaac tgtatcgatg ccttttttat cgattacacg cataccttta gcagatacgc
     3061 gcagggtgac aaaacgcttc tcgctctcaa cccagaaacg gtgagagtgc aggttcggca
     3121 ggaaacggcg tttagtcgcg ttcagtgcgt gggaacggtt gttaccggtc accggacgct
     3181 tgccagtaac ttggcagact cgggacatgt ctattctcca aaaatcaaat tagctcgagc
     3241 ttcgtatggg gtattggcgc ctcgtcaggc tttacagccc ggtcatcgca gttctatgtg
     3301 aactctcgat tgccaggccc aaatgccaaa cccgagattc tcaaaggtgg cgtagtatac
     3361 gctgactcag cgatgtgctc aagtcccgaa cagacaaaga tcccgaagga tcgcgcatag
     3421 cgggttaaat ccagccgcgt tcggcaaaag aaacatactc tccacgccca atcacgatat
     3481 ggtcgagcac gcgtaaatcc atgaactgac aactctttat tatccgttca gtaatgagtt
     3541 tatccgcttt actgggttca gcacaacccg aagggtgatt atgtgcaagg atcagcgccg
     3601 aggcgtttat ttttatcgct tagcggataa tttcccgagg atggacttca acatggttta
     3661 gcgtgccgga aaaaagacgg ctatgcgtta taacccggtg ttgtgagtcg agaaagatca
     3721 ccataaagat ctcccgctcc tcacccgtga gctggctttg taaaaattcc cgcgtcatct
     3781 ccgggctgag taaagggctt tcttcacgca tccgcacgtt gtagtaacgt cgcgccagtt
     3841 cagcaatccc ctttaactgg gcaaatttcg ctacgccaat tccatgaaca ccactaaatt
     3901 gttcatattc agaggttaac aagccataaa gagagccgaa attctccagc atctcttttg
     3961 ccagggttaa tacatcttta ccgcgcgttc cggtacgcag aaatagcgcc agcagctcga
     4021 catccgttaa ggcgctaata ccaaacttca gcattttttc gcgcggcatc aacagctgtg
     4081 cattgttttt caccttcacc tcctttgtgg tgctcgcatc ctgtcatagc cgttccgtta
     4141 aatcgacggc cagttttcaa tcctggaaag agcctcgcaa agtgaatctc aggtgaaggc
     4201 acgacaacaa aagtattgtg ataaaatcgc caacttctgg tgtcacccaa caggaaaaat
     4261 catcatgagc ctggccggta aaaaaatcgt tctcggcgtt agcggcggta ttgctgccta
     4321 taaaacccct gaactggtgc gtcgtttgcg cgatcgcggg gccgacgtcc gcgtagccat
     4381 gaccgaagcg gcaaaagcct ttatcacccc acttagcttg caggcggttt ctggttatcc
     4441 cgtttccgac agtctgcttg acccggcagc cgaagccgct atgggccata ttgagctggg
     4501 taaatgggct gatttagtga ttctcgcccc tgccacggca gatttgattg cccgtgttgc
     4561 tgccggaatg gcgaatgacc tggtatcgac gatttgtctg gctacacctg cgcctgtagc
     4621 cgtgctcccc gccatgaacc agcagatgta ccgtgccgct gccacgcagc ataatttaga
     4681 ggtgcttgct tcccgtggtt tgctcatctg ggggccagac agtggcagtc aggcttgtgg
     4741 tgatatcggt cctggacgaa tgctcgatcc gttaaccatt gtggatatgg cggtggcgca
     4801 tttttcgccc gtcaacgacc tgaaacatct gaacattatg attaccgccg gtccgacgcg
     4861 tgaaccgctc gatccggtgc gttatatctc taatcacagc tccggcaaga tgggttttgc
     4921 tatcgccgcc gctgctgccc gtcgtggcgc gaacgtcacg ctggtatcag gtccagtttc
     4981 actaccgacg ccaccgtttg ttaaacgtgt tgatgtgatg accgcgctgg aaatggaagc
     5041 cgccgtgaat gcttctgtac agcagcaaaa tatttttatc ggttgcgccg ccgtggcgga
     5101 ttatcgcgca gctaccgtgg ccccagaaaa aatcaaaaag caggccacgc agggtgatga
     5161 attaacaata aaaatggtta aaaaccccga tatcgtcgca ggcgttgccg cactaaaaga
     5221 ccatcgaccc tacgtcgttg gatttgccgc cgaaacaaat aatgtggaag aatacgcccg
     5281 gcaaaaacgt atccgtaaaa accttgatct gatctgcgcg aacgatgttt cccagccaac
     5341 tcaaggattt aacagcgaca acaacgcatt acaccttttc tggcaggacg gagataaagt
     5401 cttaccgctt gagcgcaaag agctccttgg ccaattatta ctcgacgaga tcgtgacccg
     5461 ttatgatgaa aaaaatcgac gttaagattc tggacccgcg cgttgggaag gaatttccgc
     5521 tcccgactta taccacctct ggctctgccg gacttgacct gcgtgcctgt ctcgacgacg
     5581 ccgtagaact ggctccgggt gacactacgc tggttccgac cgggctggcg attcatattg
     5641 ccgatccttc actggcggca atgatgctgc cgcgctccgg attgggacat aagcacggta
     5701 tcgtgcttgg taacctagta ggattgatcg attccgacta tcagggccag ttgatgattt
     5761 ccgtgtggaa ccgtggtcag gacagcttca ccattcaacc tggcgaacgc atcgcccaga
     5821 tgatttttgt tccggtagta caggctgaat ttaatctggt ggaagatttc gacgccaccg
     5881 accgcggtga aggcggcttt ggtcactctg gtcgtcagta acacatacgc atccgaataa
     5941 cgtcataaca tagccgcaaa catttcgttt gcggtcatag cgtgggtgcc gcctggcaag
     6001 tgcttatttt caggggtatt ttgtaacatg gcagaaaaac aaactgcgaa aaggaaccgt
     6061 cgcgaggaaa tacttcagtc tctggcgctg atgctggaat ccagcgatgg aagccaacgt
     6121 atcacgacgg caaaactggc cgcctctgtc ggcgtttccg aagcggcact gtatcgccac
     6181 ttccccagta agacccgcat gttcgatagc ctgattgagt ttatcgaaga tagcctgatt
     6241 actcgcatca acctgattct gaaagatgag aaagacacca cagcgcgcct gcgtctgatt
     6301 gtgttgctgc ttctcggttt tggtgagcgt aatcctggcc tgacccgcat cctcactggt
     6361 catgcgctaa tgtttgaaca ggatcgcctg caagggcgca tcaaccagct gttcgagcgt
     6421 attgaagcgc agctgcgcca ggtattgcgt gaaaagagaa tgcgtgaggg tgaaggttac
     6481 accaccgatg aaaccctgct ggcaagccag atcctggcct tctgtgaagg tatgctgtca
     6541 cgttttgtcc gcagcgaatt taaataccgc ccgacggatg attttgacgc ccgctggccg
     6601 ctaattgccg cgcagttgca gtaatatgac gccggatgac ttttcatccg gcgagtttct
     6661 ttaaacgcca aactcttcgc gataggcctt aaccgccgcc agatgttccg ccatttccgg
     6721 cttctcttcc agataggcaa tcagatcttt cagggtgatg atagagatca ctttgcagtt
     6781 gtaatcacgc tcaacttcct gaatcgccga aatctcgccg cgcccgcgtt cctgacgatc
     6841 gagcgaaatc aacacgccag caagcgtcgc gccattggcc tgaataatct ccatcgactc
     6901 gcgaatcgcc gttccggcgg tgatcacatc atctaccagc attacgcgtc cttgtaacgc
     6961 gctaccaacc agattgccgc cttcaccgtg gtcttttgct tctttgcggt taaagcagta
     7021 cggcaggtcc aggtcgtgat gctccgccag tgccacagcg gttgtggtgg caatcgggat
     7081 ccctttgtaa gcagggccaa acagcagatc gaactcaatg ccggaatcca ccaacgcttc
     7141 agcgtaaaaa cggcctaaca gtgccagatc gcgcccggta ttaaacagcc cggcgttgaa
     7201 gaaatagggg cttttgcgcc cggatttcag cgtaaactcg ccaaacttta acacctgctt
     7261 gctaagcgca aattcaataa actggcgctg atatggtttc atgccttcgc tcctcatctt
     7321 acttttctac agacaaaaaa aaggcgactc atcagtcgcc ttaaaaatca gtttgccagc
     7381 gccgccttct gcgtcgctac aatggattcg attccccctc gggccagcgc caacaaggtg
     7441 agtagctctt catgggtgaa cggctcgcct tctgccgtcc cctgcacttc aatgatgcgc
     7501 ccgtcttcgg tcatcactac gttcatgtcg gtctctgcgg ctgagtcttc aacgtattcc
     7561 agatcgcaaa ttgcttcacc gtttacaata ccgacagaaa ctgcggctac catccctttc
     7621 atcgggttgg ttttcagctt gccgttttcc accagcttct gtagcgcatc tgccagcgcc
     7681 acgcaggcac ccgtaatcga cgcggtacga gtgccaccat cagcctgaag cacgtcgcag
     7741 tccagcgtaa tggtgaactc acccagcgct ttcaaatcta ctgccgcgcg aagagcacgg
     7801 gcgatcagac gctggatttc cattgtgcgt ccaccctgct tacctttcgc cgcttcacga
     7861 gcgttacggg tgtgggtaga acgtggcagc atgccgtact ctgcggtgat ccagccctgg
     7921 ccctgacctt tcaggaagcg cggcacgcct tcttcaatag aggcggtaca caatactttg
     7981 gtatcgccaa attcgaccag caccgagcct tctgcatgtt ttgtatagtt acgagtcagg
     8041 gtaacgggac gcacctgatt attgctacgg cctgctggac gcatattgaa atctccggct
     8101 tgaaacaaat gtggctgcgc attatacgga cttccggcgg ttattcctat cctgacaagg
     8161 catcgatggc tataatcctt ccacctctcc ttttataaac aggaacgtct atgatccgca
     8221 gtatgaccgc ctacgcccgg cgtgaaatca agggtgaatg ggggagcgca acttgggaaa
     8281 tgcgctcggt aaaccagcgt tatctggaaa cttactttcg tctgccggag cagttccgta
     8341 gccttgaacc tgtcgttcgc gagcgtattc gttctcgcct gacgcgcggt aaagtggaat
     8401 gtaccctgcg ctatgagcca gatgttagcg cacaaggtga gctgatcctc aatgaaaaac
     8461 tggctaaaca gctggtaact gccgcgaact gggtaaaaat gcagagtgac gaaggggaaa
     8521 tcaacccggt tgatattcta cgctggccgg gcgtgatggc agcccaggag caggatctcg
     8581 acgcgattgc cgctgaaatt ctcgcggcgt tggatggtac gctggacgac tttatcgtcg
     8641 cgcgcgaaac cgaaggtcag gcactgaaag cattgatcga gcagcgtctg gaaggcgtca
     8701 ccgccgaagt ggtcaaagtc cgcgcccata tgccggaaat tctgcaatgg cagcgtgagc
     8761 gtctggtcgc gaagctggaa gatgctcagg ttcagttgga aaacaaccgt ctggagcagg
     8821 agttggttct gctggcacaa cgaattgacg ttgccgaaga gctggaccgc ctcgaagcgc
     8881 atgttaaaga gacgtacaac attctgaaga aaaaagaagc ggttggtcgt cgtctggact
     8941 ttatgatgca ggagttcaac cgtgagtcga acactcttgc gtcgaaatct atcaatgccg
     9001 aagtgacaaa ctccgccatc gagctgaaag tgttgattga gcagatgcgc gagcagattc
     9061 agaatattga gtaactcgta acaccaattc tgacttttca gccttcacaa atgccgccca
     9121 ctcaaacaga gcggcatttt tcttccccgc aacttcaatt ctgttttgcg tgcctgctcc
     9181 agattttgcg atgttttttt gcccagcaca ctgagaacgt gagatactca caactgtata
     9241 taaatacagt tacagattta ctttctttgc aatcgatatc acatggagtg ggcaatgaac
     9301 gaacatcatc aaccttttga agagataaaa ctgattaatg caaacggagc agaacaatgg
     9361 tcagcaagac aacttgggaa actactgggt tattcagagt atcgtcactt tatacctgta
     9421 ttaacgcgcg ccaaagaggc ctgtgagaac agtggtcaca caattgatga ccatttcgag
     9481 gagatcctcg atatggtcaa aattggctca aatgccaaaa gagcattaaa agacatcgta
     9541 ctctcccgct atgcctgtta cctggtagta caaaacggcg accccgcgaa accggtcatt
     9601 gcggcagggc agacttattt tactatccag acccgacggc aggagcttgc tgacgatgaa
     9661 gcatttaagc aacttcgtga agacgaaaag cgtctgtttc tgagaaatga gttgaaagaa
     9721 cataacaaac aattggttga ggcagcacag caagctgggg tagcgacagc tactgatttc
     9781 gccatatttc agaatcatgg ttaccagggg ctgtatggtg gattagatca gaaagccatc
     9841 caccaactga aggggctgaa aaagagtcag aagatcctgg atcatatggg ttcaacagaa
     9901 ctggcggcta atctctttcg agctacccaa acagaagaaa aactcaagcg ggatagcgtg
     9961 aattcaaaac agcaagctaa taccacacac tttgacgtgg gtagcaaggt gaggcaaacc
    10021 attcaggaac ttggcggaac catgcctgag gagttgccga ccccgcaggt cagcatcaag
    10081 cagttggaaa acagtgtaaa aattacagag aagaaatagc agaaacgaaa aactcttttt
    10141 gttgattgag acacccgatg cgtaagccaa ggtccaggtg cagtgaacac aatggctaaa
    10201 cattgcacct ttcttttccc ccctcagttt taacctattt tttcttatgc attttctcag
    10261 acaagaagtc agaagaatgc atctacgcta cagaaaatag cgatttcaca taactaaaag
    10321 ttatcaattt cctctcacct taaaaaatat caatcgtgac aatgcgcaca aatcgctacc
    10381 ctgccagaca gatttttagg gagagaacca tgctgttaca cattttgtat ttggttggca
    10441 ttactgccga agccatgacg ggggcgctgg cggccgggcg acggcgcatg gatacatttg
    10501 gcgtaattat tattgcaacc gccaccgcaa ttggcggagg gtcagtgcgc gatattctgc
    10561 tgggccacta tccgctcggc tgggtcaaac acccggaata tgtgattatc gtcgctaccg
    10621 ccgcagtact caccactatc gttgctcctg taatgcctta cctgcgcaaa gtgtttctgg
    10681 tgctcgatgc gctcggactg gtggtctttt ctatcatcgg cgcacaggtt gcactggata
    10741 tggggcacgg cccaattatt gccgttgtcg cggcggtaac caccggtgta tttggcggcg
    10801 ttttgcgcga tatgttctgt aaacgcattc cgctggtctt tcaaaaagag ttgtatgccg
    10861 gtgtctcatt tgcctccgcc gtgctgtaca tcgcgctgca acactatgtt tctaaccatg
    10921 atgtggtgat tatctccacc ctggtattcg gcttttttgc tcgtttactg gcgctacgtc
    10981 ttaagctggg attaccggtc ttttactaca gccacgaagg tcactaaggt tcaaaacctg
    11041 tgatctgctg ggcagccagc caactgccca gcttcttgat ttgcgcattt tccttccatt
    11101 caataacctg tctggcgcgc ccagatccag tccccggcag ctgctgccag aactgctccg
    11161 tgctaaataa aagttgcgac caggatcgtt catcactggc attaagcgcc gcccgggtta
    11221 gcggtattcc cattgccatc acccagcgag taaaaggctg gttacgagcc agattaaact
    11281 gatgccataa ctgcgcactt ttacttttcg cgatccccgg cgtgttctgt aattgctctg
    11341 gcgttaataa aagccaggaa aagatatgct caaagcgatg agtctgatgc agcgcgcgcc
    11401 aaccggcctc accaatgcca tccagcccca gaacctgttt tgaccccagc cagactaagc
    11461 gtgaaatgaa ctgttcctga caaacatcag aagcaaagta gcaggtcaac gagttaaagc
    11521 ggttttctgg tggtgtcggt tttgtacgtt ctgcaccgcg ccagaccaca tcatcaatgc
    11581 gaggaatacc ctgaccggca aggctgacga ggatctgatc accaggcgca atatcccact
    11641 cctgccagcg cctgacggaa ccaatattca cccgctggac ttttttatca tccagcatga
    11701 caggtgcgag tgacgcaacc accgatattt taccgctctt acccaccgca aactgaatcg
    11761 ccttcacttc ggcaacctga gctacaggtt gatatttcca ggccaccagc cactccgcct
    11821 ggcccggtag ccaatgacgg gattcgggtt ccttagcccc tcgcacaacc acgccatcgg
    11881 tgacgaaggg taatttcgct ttccaccact cattgcgtac gcgcgcaacc tcatcagcat
    11941 ttttaaccgc acgggtatac ctctgcgtta gagtaaaacc tgcggtagcc agctccttta
    12001 aacgatcggt cattaactgc ggtccatccg gccatgccca gacaaaaaca gccagtgagt
    12061 ttagcgtgtc gctattcccc tggcgcatca tcaggccagc aacttttgcg cgggcattta
    12121 ttccccccat ttgttgttgg atatgcccct cgcgctggag aaatatttcc ccctgaagcg
    12181 tactgttggc taaaggcccg ctaacggttt gcggcacagc ggaaattaag ctaactttct
    12241 gcgtccagtc ctcgcctttc aggccattac cgcgactgat tgctttgttc agtttcccgt
    12301 cccgataaac cagggttacc gcaacgccat caacttttgg ctgcacccaa agatcgctac
    12361 gttctcgcat ccacagactt aatgcgtttt tatccgccat tttgcgtaca cccgtatggg
    12421 caaccggatg cataaccgca ccatttaaag gcggcatcat cacgtcgcgg ggctcgctca
    12481 caaagcagcg ttgccactgc gtaagacggg cgcttaactg atcgtaaaca ccgtcttcca
    12541 cctcactttt tccttccttc cagtagtcat cgtcccactg ttttatttgc tgttgcaggc
    12601 gggaaatttc ttcctgtgct ctggatggcg accaggccgg acagaccgcc cacacagatg
    12661 attgccagca caagatactt attaatatcg ccatccatac tttcatcttc acctccgctg
    12721 tagtcaggct ggcagatata caacgtggtg aaggcaaagc cgagtggcaa aaacggagtc
    12781 tgcgaggacg cttcctgaga atcgtcttta ttgcagtaaa tcacaggcaa atgcggaaac
    12841 agctacgcaa aacgcaacaa ctttgcgcaa aaagtgtgag caagggctac gtcacatggc
    12901 cgcgccgtgt ataataagct cgtatgtagg ctttatttcg ctaatcacat acgaaagata
    12961 ctcatggctc aaggcacgct ttatattgtt tctgccccca gtggcgcggg taaatccagc
    13021 ctgattcagg ctttattaaa aacccaaccg ttgtatgaca cccaggtttc tgtttcacac
    13081 accacacgcc aaccgcgtcc tggtgaagtc cacggtgaac attatttctt tgttaatcat
    13141 gatgaattta aagaaatgat tagcagagat gcgttcctcg aacacgcaga agtttttggt
    13201 aattactatg gcacttcgcg tgaggccatt gagcaagtac tggcgaccgg tgtcgatgtt
    13261 tttctcgata tcgactggca gggcgcgcag caaattcgcc agaagatgcc gcacgcgcgg
    13321 agtatcttta ttttaccgcc gtccaaaatt gaactggacc gccgtctacg cggtcgcggt
    13381 caggacagcg aagaggtcat tgcaaagcgt atggcgcaag ctgttgcaga aatgagccat
    13441 tacgccgaat atgattatct gattgtgaat gatgacttcg ataccgcgtt gaccgatttg
    13501 aagaccatta ttcgcgccga acgtctgcgc atgagccgcc aaaagcagcg tcatgacgct
    13561 ttaatcagca aattgttggc agactgaacc tgatttcagt atcatgccca gtcatttctt
    13621 cacctgtgga gctttttaag tatggcacgc gtaactgttc aggacgctgt agagaaaatt
    13681 ggtaaccgtt ttgacctggt actggtcgcc gcgcgtcgcg ctcgtcagat gcaggtaggc
    13741 ggaaaggatc cgctggtacc ggaagaaaac gataaaacca ctgtaatcgc gctgcgcgaa
    13801 atcgaagaag gtctgatcaa caaccagatc ctcgacgttc gcgaacgcca ggaacagcaa
    13861 gagcaggaag ccgctgaatt acaagccgtt accgctattg ctgaaggtcg tcgttaatca
    13921 caaagcgggt cgcccttgta tctgtttgaa agcctgaatc aactgattca aacctacctg
    13981 ccggaagacc aaatcaagcg tctgcggcag gcgtatctcg ttgcacgtga tgctcacgag
    14041 gggcaaacac gttcaagcgg tgaaccctat atcacgcacc cggtagcggt tgcctgcatt
    14101 ctggccgaga tgaaactcga ctatgaaacg ctgatggcgg cgctgctgca tgacgtgatt
    14161 gaagatactc ccgccaccta ccaggatatg gaacagcttt ttggtaaaag cgtcgccgag
    14221 ctggtagagg gggtgtcgaa acttgataaa ctcaagttcc gcgataagaa agaggcgcag
    14281 gccgaaaact ttcgcaagat gattatggcg atggtgcagg atatccgcgt catcctcatc
    14341 aaacttgccg accgtaccca caacatgcgc acgctgggct cacttcgccc ggacaaacgt
    14401 cgccgcatcg cccgtgaaac tctcgaaatt tacagcccgc tggcgcaccg tttaggtatc
    14461 caccacatta aaactgaact cgaagagctg ggttttgagg cgctgtatcc caaccgttat
    14521 cgcgtaatca aagaagtggt gaaagccgcg cgcggcaacc gtaaagagat gatccaaaaa
    14581 atcctctcgg aaatcgaagg gcgtttgcag gaagcgggaa ttccctgccg cgtcagtggt
    14641 cgcgagaagc atctttattc gatttactgc aaaatggtgc tcaaagagca gcgttttcac
    14701 tcaatcatgg acatctacgc tttccgcgtg atcgtcaatg attccgacac ctgttatcgc
    14761 gtgctcgggc aaatgcatag cctgtacaag ccgcgtccgg gtcgcgtgaa agactacatc
    14821 gccattccaa aagcgaacgg ctatcagtct ttgcacacct cgatgatcgg cccgcacggt
    14881 gtgccggttg aggtccagat ccgtaccgaa gatatggatc agatggcgga gatgggtgtt
    14941 gccgcgcact gggcttataa agagcacggc gaaaccagca ccaccgcgca aatccgcgcc
    15001 cagcgctgga tgcaaagcct gctggagctg caacagagcg ccggtagttc gtttgaattt
    15061 atcgagagcg ttaaatccga tctcttcccg gatgagattt acgttttcac accggaaggg
    15121 cgaattgtcg agctgcctgc cggtgcaacg cccgtcgact tcgcttatgc agtgcatacc
    15181 gatatcggtc atgcctgcgt gggcgcacgc gttgaccgcc agccttaccc gctgtcgcag
    15241 ccgcttacca gcggtcaaac cgttgaaatc attaccgcac cgggcgctcg cccgaatgcc
    15301 gcgtggctga actttgtcgt tagctcaaaa gcgcgcgcca aaattcgtca gttgctgaaa
    15361 aacctcaagc gtgatgattc tgtaagcctg ggccgtcgtc tgctcaacca tgctttgggt
    15421 ggtagccgta agctgaatga aatcccgcag gaaaatattc agcgcgagct ggatcgcatg
    15481 aagctggcaa cgcttgacga tctgctggca gaaatcggac ttggtaacgc aatgagcgtg
    15541 gtggtcgcga aaaatctgca acatggggac gcctccattc caccggcaac ccaaagccac
    15601 ggacatctgc ccattaaagg tgccgatggc gtgctgatca cctttgcgaa atgctgccgc
    15661 cctattcctg gcgacccgat tatcgcccac gtcagccccg gtaaaggtct ggtgatccac
    15721 catgaatcct gccgtaatat ccgtggctac cagaaagagc cagagaagtt tatggctgtg
    15781 gaatgggata aagagacggc gcaggagttc atcaccgaaa tcaaggtgga gatgttcaat
    15841 catcagggcg cgctggcaaa cctgacggcg gcaattaaca ccacgacttc gaatattcaa
    15901 agtttgaata cggaagagaa agatggtcgc gtctacagcg cctttattcg tctgaccgct
    15961 cgtgaccgtg tgcatctggc gaatatcatg cgcaaaatcc gcgtgatgcc agacgtgatt
    16021 aaagtcaccc gaaaccgaaa ttaatgtttt atgaacccaa cacgttatgc acgcatctgc
    16081 gaaatgctcg ccaggcggca gcctgatctg accgtctgca tggagcaggt ccacaaacct
    16141 cataacgttt ctgcgattat tcgtaccgca gatgccgttg gcgtacatga agttcacgcc
    16201 gtctggcctg gtagccgcat gcgcaccatg gcttcggcag cggcgggtag taacagctgg
    16261 gtacaggtga aaacacaccg caccattggc gatgccgtcg ctcatctcaa aggccagggc
    16321 atgcagattc tggcaaccca tctttctgat aatgctgtcg atttccgcga aattgattac
    16381 actcgcccga cctgcatttt gatgggacag gagaaaacgg gcatcacgca ggaagcattg
    16441 gccctggcgg atcaggacat catcattccg atgatcggca tggtgcagtc gctgaatgtt
    16501 tccgttgcct cagccctcat tctttacgaa gcccagcgtc agcggcaaaa tgcaggcatg
    16561 tacctgcgtg aaaacagcat gttgccggaa gcagagcaac aacgcctgtt gtttgaaggc
    16621 ggctatccgg tgctggcgaa agtcgcaaaa cgcaaaggcc tgccttatcc ccacgtcaat
    16681 cagcaaggcg agatcgaagc tgatgccgac tggtgggcta ctatgcaggc tgcagggtaa
    16741 gtgccatgaa aggtcgcctg ttagatgctg tcccactcag ttccctaacg ggcgttggcg
    16801 cagcacttag taacaaactg gcgaaaatca acctgcatac cgtgcaggat ctactcttac
    16861 accttcccct gcgctacgaa gatcgcaccc atctctaccc catcggagaa ctactgccgg
    16921 gcgtttatgc cacggtggaa ggcgaagtgc tgaactgcaa tatctccttc ggcggtcggc
    16981 ggatgatgac ctgccagatc agcgacggtt ccggcatcct caccatgcgc tttttcaatt
    17041 tcagcgcggc aatgaaaaat agcctggcga cgggccgccg tgtactggct tatggcgaag
    17101 caaagcgcgg taaatatggt gcggagatga tccacccgga ataccgcgtg cagggcgatc
    17161 tcagcacgcc agaattacag gaaacgctca cgccggttta tccaacaacg gaaggcgtaa
    17221 agcaggccac gctgcgtaaa ttaaccgacc aggcgctgga tctgctcgac acctgcgcca
    17281 ttgaagaact cctgccgccg gaactgtcac aaggaatgat gacgctaccg gaagcgttgc
    17341 gcactttgca ccgcccgcca ccgacgctac agcttagcga tctggaaacc gggcagcatc
    17401 cggcgcaacg tcgtctgatt ctggaagaac tgctggcgca caacctcagc atgttagcct
    17461 tacgtgccgg agcacagcgt tttcatgccc agccgctgag cgccaatgac acgctgaaaa
    17521 ataaactcct cgccgcctta ccgttcaagc caacgggcgc acaggcacgc gtagtggcgg
    17581 agatcgagca tgatatggcg ctggatgtgc cgatgatgcg cctggtacag ggcgatgtag
    17641 gttccggtaa aacgctggtc gccgccctcg ccgcgctgcg tgcgattgcc cacggcaaac
    17701 aggtggcact gatggcacca accgaattac tcgccgagca gcacgccaat aacttccgca
    17761 actggtttgc accgctcggt atcgaagtgg gctggctcgc cggtaagcag aaaggcaaag
    17821 cgcgactgtc tcagcaggaa gccatcgcca gcggtcaggt gcagatgatt gtcggtacac
    17881 acgccatctt ccaggaacag gtgcagttta acggcctggc gctggtgatt atcgacgaac
    17941 agcatcgttt tggcgtgcat cagcgtctgg cattgtggga gaaaggccag cagcagggct
    18001 tccatccgca tcagttgatc atgaccgcca cgccgatccc ccgcacgctg gcaatgacgg
    18061 cgtatgccga tctcgatacc tcggtgatag atgagctgcc gccaggccgc acgccagtga
    18121 ctacggtcgc tattcctgat acccgccgta ccgacatcat tgaccgcgtg cgccacgcct
    18181 gcataactga aggtcgtcag gcgtactggg tttgtacgtt gattgaagag tcagagttac
    18241 tggaagcgca ggcggcggaa gctacctggg aagagttgaa actggcgcta ccagaattga
    18301 atgttggcct ggtacacggg cggatgaaac cagccgagaa acaggcggtg atggcgtcgt
    18361 ttaaacaagg tgagctacat ctgctggttg ccactaccgt tattgaagtc ggtgttgatg
    18421 tgcctaacgc cagcctgatg attatcgaaa accctgagcg tctgggtctg gcgcagttac
    18481 accagctgcg tgggcgcgta ggtcgtggcg cggtggcttc tcactgcgtg ctgctctaca
    18541 aaacgccgct ttctaaaacg gcgcaaattc gcctgcaagt gctgcgcgac agtaacgacg
    18601 gttttgtgat tgcgcaaaaa gatctggaga ttcgcggccc tggcgaattg ttaggcacgc
    18661 gtcagacggg taatgctgaa tttaaagtgg cggatttact gcgcgatcag gcgatgatcc
    18721 cggaagttca gcgcctggcg cgacatattc acgaacgtta cccacaacag gcaaaagcct
    18781 tgatagaacg ctggatgccg gagacagaac gttactcgaa tgcgtaaaag gcggcggtga
    18841 gaagaccgcc gcttcatcgg ttaaccggca aaaatcggca acattaaata cagcttaatc
    18901 accagcgcat tgacgatatc gataaagaac gcaccgacca tcggcaccac caaaaacgcc
    18961 atgtgcgacg ggccaaagcg ttcagtgatc gcctgcatgt tggcgattgc cgttggcgtt
    19021 gcaccgaggc caaaaccaca gtgacccgca gccagcactg ccgcatcgta gtttttgccc
    19081 atcatgcgcc aggtaacgaa gatggcatac aacgccatga agatggtctg taccaccaga
    19141 atcgccagca tcggcagtgc cagcgaagcc agctcccaca gtttcagccc catcaacgcc
    19201 atcgccagga acaacgacaa gcttacgtta cccagcacgg ataccgcacg ctcaaagacg
    19261 cggtaaaagc ccattattga cagaccgttg ctcagaatca cgccaacaaa cagtacacag
    19321 acgaaggtcg gcagttcaaa agcagtgcca gccaaaagtt gcgcaacaat tttccccacc
    19381 gtcaggcaga tagcaatcag cgcgatagtt tcaatcagca ccaacgaggt gatcatgcgt
    19441 cccacatccg gcttttcaaa cgccgtcggg acttcctggt catccggaat accgttcggc
    19501 gtggtggagt gtttcaccag atagcgcgcc accggaccgc caatcaagcc gcccagcacc
    19561 agaccgaacg ttgcacaggc catcgccact tccgtcgcat tggtgaagcc ataacgttca
    19621 atgaacaatt tactccacgc agcgcccgta ccgtgaccgc cggaaagcgt aatagaaccg
    19681 gccaacagcc ccatcagcgg atcaagccct aacaggctag ccataccaat accaatggca
    19741 ttttgcatca ccaacagacc aacaaccaca atcaagaaga tgccaaccac acgcccaccg
    19801 gcacgcaaac tggcaatgtt ggcgttcagg ccaatggtgg cgaagaaagc cagcattaac
    19861 ggatcgcgca gggacatatc aaagttgact tcccagccca tgcttttttt cagtactagt
    19921 agcgccagcg ccaccaacaa accacccgca acaggttccg gtatggtgta tttcttcaaa
    19981 aaggagacgg aatggaccaa cttacgcccg agcagcaacg tcagcgttgc ggcaacaagc
    20041 gttgctaaag tatcgagatg aaacatagtt actcctttgt atccgcatca ctctttcata
    20101 ctcgtcatac ttcatcgcgc agtggcaact tcatgaacta tgacgggtat cgagttatgc
    20161 tttcctgggt caaattctca gctgttaatg aacaaatggc atggatttta agcagaaaaa
    20221 taccaaaagt tatataaaaa agtgcattta tctcatttaa atatttagat gaggatgtgg
    20281 caaacgtttg cttttctctt tcggtcagct aaaatgcccg ctttgctacc acgggattgt
    20341 tttcaatgtc tgtttccacc ctcgagtcag aaaatgcgca accggttgcg cagactcaaa
    20401 acagcgaact gatttaccgt cttgaagatc gtccgccgct tcctcaaacc ctgtttgccg
    20461 cctgtcagca tctgctggcg atgttcgttg cggtgatcac gccagcgcta ttaatctgcc
    20521 aggcgctggg tttaccggca caagacacgc aacacattat tagtatgtcg ctgtttgcct
    20581 ccggtgtggc atcgattatt caaattaagg cctggggtcc ggttggctcc gggctgttgt
    20641 ctattcaggg caccagcttc aactttgttg ccccgctgat tatgggcggt accgcgctga
    20701 aaaccggtgg tgctgatgtt cctaccatga tggcggcttt gttcggcacg ttgatgctgg
    20761 caagttgcac cgagatggtg atctcccgcg ttctgcatct ggcgcgccgc attattacgc
    20821 cgctggtttc tggcgttgtg gtgatgatta tcggcctgtc gctaattcag gtggggctga
    20881 cctccattgg cggcggttac gcagccatga gcgataacac cttcggcgca ccgaaaaatc
    20941 tgctgctggc aggcgtggtt ttagccttaa ttatcctgct taaccgtcaa cgtaaccctt
    21001 acttacgcgt ggcctcactg gtaattgcga tggcggccgg atatgcgctg gcgtggttta
    21061 tgggcatgtt gccagaaagc aacgaaccga tgacgcaaga actgattatg gtgccaacgc
    21121 cgctctatta cggtcttggc attgaatgga gtctgctgct gccgctgatg ctggtcttta
    21181 tgatcacttc gctggaaacc attggcgata tcacggcgac ctctgacgtt tccgaacagc
    21241 cagtgtccgg tccgctgtac atgaaacgcc tgaaaggcgg cgtgctggca aacggcctga
    21301 actcgtttgt ttcggcggtg tttaacacct tcccgaactc ctgcttcggg caaaacaacg
    21361 gagtgatcca gttgactggt gttgccagcc gctatgtcgg ttttgtcgtc gcgctgatgt
    21421 tgatcgtgct gggtctgttc ccggcagtga gcgattttgt acaacacatt ccagaaccgg
    21481 ttctgggcgg cgcaacgctt gtaatgtttg gcaccatcgc cgcctccggt gtgcgtatcg
    21541 tttctcgtga gccgctgaac cgtcgggcga ttctgattat cgcgctgtcg ctggcggttg
    21601 gtctgggcgt gtctcagcag ccgctgattt tgcagtttgc ccctgaatgg ctgaaaaacc
    21661 tgctctcctc cgggatcgcc gcgggcggta ttactgccat cgtgctgaat ctgattttcc
    21721 caccagaaaa acagtaattc actggcggcg gtaagataat taccgccgca gtctcacctc
    21781 tttcaccatt cccgccttga ggattgcgcg taaatcgtgc ataactccct tatgtgcatt
    21841 tcacgggatg gaagaccatg aaatttattg ggaagctgct tctctacatt ctcatcgctc
    21901 tgttagtggt gatcgctggc ctctattttc ttctgcaaac ccgctgggga gcagaacata
    21961 tcagcgcatg ggtttccgag aatagcgact atcatctggc cttcggggcg atggatcacc
    22021 gtttttccgc gccatctcat atcgtgctgg agaatgtcac gtttggtcgt gatggtcagc
    22081 ccgcgcccct ggtggcaaaa agtgtcgaca ttgcgctaag cagtcgccaa ctgaccgaac
    22141 cacgccatgt cgataccatc ctgctggaaa acgggacgct gaatctcacc gaccagaccg
    22201 cgccgctacc gttcaaagcc gatcgtctgc aactgcgtga tatggcgttt aatagcccga
    22261 atagcgaatg gaaactgagc gcgcagcggg taaatggcgg cgtggttccg tggtcaccag
    22321 aagccggtaa agtgctgggt acgaaggcgc agattcagtt tagtgccgga tcgctttcgc
    22381 tcaatgatgt tcctgccacc aatgtactga ttgaaggcag tattgataac gatcgcgtta
    22441 cgctgactaa cctgggtgcc gacatcgccc gcgggacatt aaccggaaac gcgcagcgta
    22501 acgccgacgg cagctggcaa gtggaaaatc tgcgcatggc ggatatccgt ctacaaagcg
    22561 aaaaatcgct aaccgacttc tttgcgccat tacgctctgt cccgtcgttg cagattggtc
    22621 gcttggaagt catcgatgct cgtctgcaag gtccggactg ggcggtgacc gacctcgatc
    22681 tcagcttgcg caacatgacc ttcagtaaag atgactggca gacacaggaa ggcaaactgt
    22741 cgatgaacgc cagcgagttc atttatggtt cgctgcattt atttgacccg attataaacg
    22801 cggaattttc cccgcagggc gtagcgctgc gccagttcac cagccgctgg gaagggggta
    22861 tggtcagaac gtcagggaac tggctgcgtg acggtaaaac gttgatcctt gatgatgcgg
    22921 caattgccgg gctggaatat accttgccga aaaactggca acagttgtgg atggaaacga
    22981 cacccggttg gttaaacagc ctgcaactga agagatttag cgccagccgc aatctgatca
    23041 ttgatatcga ccctgacttc ccgtggcagc tcaccgcgct cgatggttac ggtgccaacc
    23101 tgacgctggt taccgatcat aaatggggcg tctggagtgg ctcggcgaat ctgaatgccg
    23161 ccgccgcgac attcaatcgt gttgatgttc gtcgcccgtc gctggcgctg accgccaaca
    23221 gcagcacggt gaatatcagc gaactgagtg catttactga aaaaggcatt ctggaagcca
    23281 ccgcaagtgt ttcacaaacg ccacaacgtc agacccatat cagcctgaat ggacgcggtg
    23341 tgccggtgaa tattttgcaa caatggggat ggcctgaatt acagttgact ggcgacggca
    23401 atattcagct taccgccagt ggagatattc aggccaatgc cccgctgaaa cctacggtta
    23461 gcgggcaatt gcatgccgtg aacgccgcaa agcagcaagt gactcaaacc atgaatgcgg
    23521 gcgtcgtttc cagcggtgaa gttacatcga cggagccggt gcggtaaaca tcaatctcgc
    23581 cgggtacgac tcagacgtac ccggcattcc gtcaatagat tacaacgtaa ttgtcagcgc
    23641 attcccttga ggcgtcacca ccagcccctg ctcactttca gcctgcgaac cgccttgcag
    23701 accatttact ttcacaacat tgcgcaagca cagcgtccag ttcttcgcct cgcccgcacc
    23761 agtcacagta atcgtgttgc cagtacgtgc tgcttttaaa gtaaagatca ccgatccgtc
    23821 agcagcaggc acttcacaga cggcttcatg cccgtcttgc agattaaaga ggtggaatgc
    23881 cgtgccttcg tgccacacgt aatcgggacg ttgatcgttg ttgcccagcg ccagtagagt
    23941 gttatcacgc acataaacgg gcagactcag gaagccgtgc tgctgtttat gccagcgact
    24001 accgtcgagt tcatcgttgt gccacaggtg tgtccagcga ccttccggca ggtagaactg
    24061 cacatcgccc gcttcagtga acaccggcgc aaccatcacg ttgtcgccta acatgtattg
    24121 acggtcaagg taatcacaag ccggatcgtc cgggaactcc atcatcatgg cccgcatcat
    24181 cggcgtaccc cgcgcgttcg cacgcgcagc ttcacgatac agatacggca tcatgcggca
    24241 tttcagttgc gtgaagaagc gcaccacatc acaggactca tcatcgtagg cccacggcac
    24301 acgataagat ttgctaccgt gtaaacggct atggctggag agcaaaccaa acgcgcacca
    24361 gcgtttgtaa acgtgcgccg gagcggtatt ttcaaagccg ccgatatcgt ggctccagaa
    24421 gccaaaacct gaaaggccaa tagacaaacc accgcgcagg ctttccgcca ttgattcgta
    24481 gttagcgtaa caatcgccac cccagtgtac cgggaatttc tgcgcaccga cggaggccga
    24541 gcgggcaaac aagacagctt cttcctcacc aacggtgtcc ttgagcacgt tccacaccag
    24601 ttcgttgtag atgtacgcat aatggttatg cattttctgc ggatcggaac cgtcaaacca
    24661 ctgaacatca gttgggatac gttcgccaaa gtcggtctta aagcaatcaa cgcccatcgc
    24721 gaccagacct ttcagtttgt cggcgtacca tttgcaggca tccggattgg taaagtcata
    24781 aatcgccaga cctggctgcc atttatccca ctgccatagc gaaccgtccg ggcgtttgag
    24841 taaatagcct ttctcttgta actctttaaa gacgggggat ttttgaccga tataggggtt
    24901 aatccagacg cagattttca gccctttcgc tttcaggcgg cggatcatcc cttccgggtc
    24961 agggaaagtc agcgggtccc actcaaaatc gcaccactgg aaggctttca tccagaaaca
    25021 gtcaaagtgg aaaacatgca gcggcagatt gcgttccgcc ataccatcga taaagctgtt
    25081 taccgtcgct tcgtcgtagt tggtggtaaa tgaagtggtt agccacaggc cgaaggacca
    25141 cgggggcggc agcgccggac gaccagtaaa gcgggtataa cgatcaagta ccgctttcgg
    25201 cgtcgggccg tcgataacaa agtattcgag atattcactc tcaacgctga actgcacttt
    25261 ggagactttc tccgatccca cttcaaaaga gacacactgg ggatgattga ccagtacccc
    25321 ataaccacgg ttagtcatgt agaacgggat atttttatac gcctgttcag tacttgtgcc
    25381 gccgtcccgg ttccaggtct ctaccgtctg gccattgcgc accagggcag taaagcgctc
    25441 tcccagaccg taaactgttt cgccaacgcc aagatcaagc cgctcaaaca tataattgcg
    25501 ttgattattc gtgtcctgca cgtagccatt atttttcacc tgactaccgg taatacgttc
    25561 gccgttgcgc agaaaatcca gtgaccagaa ctcacctttg ctgacacgcg cgcttaagtt
    25621 gccactttta aactcagcat aacgttctgt gttttcgatt gtgaccttca cgtcctgcaa
    25681 aatattgagc ggataatgag gaccgttatt cagcgccccc tgaaaatact caatccgcac
    25741 accgacaata ccttcctgtg gggagaaaaa gcgcagcgta aataaaggcg tatcaagctg
    25801 ccaggtacgt tcacgcacat cacggggggc agcatagacc accatttcat tatcctgctg
    25861 ttcaacctcg aatacctgaa gcgggtgaat caaattgagg ccaggttgaa tcaaccagtt
    25921 tccatcgcta attttcattg ccgttcctta gttctgcact tcttgagagg tgaattgttg
    25981 ctggcaacgg cgtttaccct gagccagctg ttccataacg gttttcagat tgtgcgtcgt
    26041 gagtgagtag tagcgtttag cgataatcgc gctcagcaaa taacagatcg ccggaacaat
    26101 cgtgaatagc gcaataatga tgctaatcgt ggcgctgttc tgcgcttttt ctgccgcatc
    26161 atatccgcca taagccagca tccagccgat aagagcgccg ccgaaggcca accccagttt
    26221 gagcacaaac agcgtgccag caaaactgat cccggtcagg cgtttaccat tgcaccactc
    26281 gccgtagtcg acggtatcga acatcattac ccactggata ggtgtcacca gttgatgcaa
    26341 cacaccaatc acgaagatga agacaaacat agtgatgctg gcctgcatgg gaacaaagaa
    26401 catcgcgagg ctaatcactg ccagcagggc gttcgtccac cagaagatag tgactttaca
    26461 tttccagtcg gtcagaggtt ttgccagtgc ggaaccaatc aggttaccca cgcaataagt
    26521 ggtgagaaaa gcgacaaaca cttccggcgt gcccaaaatc catgtgacgt aatacatcat
    26581 cgccccaccg cgtacgcaca ccgccaggat attgaaaatg gttagtaaac cgacaatccg
    26641 ccactggtcg ttttgccaga tatcacgtaa atcttcccgc atagacgttg ttgtaggtgg
    26701 tgcttcaacg cgttctttag tggtgaagaa acaaaatgcc agcatcatga atgccaccac
    26761 ggaaaggacc acgataccgc cctggaaacc gagtggttta ttatcaccgc caattaaatt
    26821 aaccagtggc atcatcagaa cagtagaaag catgcctccc gcggtcgcca gcacaaaacg
    26881 ccaggattgc agcgagatac gctgagtcgg gtcattggtg attacaccac ccaatgcgca
    26941 gtaagggata ttgacgacgg tataaagtaa ggtaagttag ggtgtaagta attgctgcat
    27001 agatcatttt gccgttcata ctgagatctg gcgtgctata ggccagtaca cagacgatcc
    27061 cgaatggcag tgcgccaaac agtacccacg gacgaaattt accccagcga gagcgcgttc
    27121 gatcggccaa caaccccatg caaggatcgg aaatcgcatc cagtgcacga gcgaccaaaa
    27181 acatggttcc gacaaatccg gcaggaatgc caaaaatatc ggtataaaag aacatcatat
    27241 ataacattac gttatcgaaa ataatgtggc tggcggcgtc tcccatgcca taaccaattt
    27301 tctctttaac ggacaacact tcactcttca tctgcttttc cttgatgcta taggggtgct
    27361 gagaccgata acgcattttg taaaccatcc ctgtgagagc gaatattccg atatctggtt
    27421 attaatttat gattcttgtt ttatgtgatc gtggtagcgt taattccgct catatatcat
    27481 tgtaaaatat gggttttata taaattataa tgctttcgtg ataatacgct gcgtttatta
    27541 ggcggaaaaa actgatctgg gggatgtaga aactcaagga agtagctata atgcgccccg
    27601 cctccatgta gcaatcgagg cgcggaagat cgtcgtctcc ggtgaggcgg ctggacttca
    27661 aatccagttg gggccgccag cggtcccggg caggttcgac tcctgtgatc ttccgccagt
    27721 ttacctcttc tgaactctcc caatgtcaat aaatctcttc taatcccttg tatatcagta
    27781 tttatttgta tcctgaggtc aacctacttc accccgtatc aagtttcttt tgggggtact
    27841 ttagggggtg ttatttccta ttgaaataca agcaccccca taataggaaa aatgtgatac
    27901 ataacaacgg cttttaacaa tatctttgtt atacaagggg tgtattgggg gtatcaggtt
    27961 agttactggc agaaatcccc ctcaggaaca tgaattcatc aaaagccgga ggatgtcatg
    28021 gcattaaccg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatggagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcat
//
LOCUS       DAJTYG010000046        28443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTYG010000046 DAJTYG010000000
VERSION     DAJTYG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.046"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..1188
                     /gene="pdeC"
                     /locus_tag="OO568_002153"
                     /pseudo
     CDS             1..1188
                     /gene="pdeC"
                     /locus_tag="OO568_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            complement(1191..1514)
                     /gene="soxS"
                     /locus_tag="OO568_002154"
     CDS             complement(1191..1514)
                     /gene="soxS"
                     /locus_tag="OO568_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6641498.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1600..2064
                     /gene="soxR"
                     /locus_tag="OO568_002155"
     CDS             1600..2064
                     /gene="soxR"
                     /locus_tag="OO568_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6641499.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2768..4117
                     /gene="ghxP"
                     /locus_tag="OO568_002156"
                     /gene_synonym="yjcD"
     CDS             2768..4117
                     /gene="ghxP"
                     /locus_tag="OO568_002156"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6641500.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4268..5917
                     /locus_tag="OO568_002157"
     CDS             4268..5917
                     /locus_tag="OO568_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6641501.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6071..7363)
                     /locus_tag="OO568_002158"
     CDS             complement(6071..7363)
                     /locus_tag="OO568_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6641502.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7541..9190)
                     /gene="actP"
                     /locus_tag="OO568_002159"
     CDS             complement(7541..9190)
                     /gene="actP"
                     /locus_tag="OO568_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6641503.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLITERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVMLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLRLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9187..9501)
                     /locus_tag="OO568_002160"
     CDS             complement(9187..9501)
                     /locus_tag="OO568_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR6641504.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9701..11659)
                     /gene="acs"
                     /locus_tag="OO568_002161"
     CDS             complement(9701..11659)
                     /gene="acs"
                     /locus_tag="OO568_002161"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR6641505.1"
                     /translation="MSQIHKHTIPANITDRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPKAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12052..13488
                     /gene="nrfA"
                     /locus_tag="OO568_002162"
     CDS             12052..13488
                     /gene="nrfA"
                     /locus_tag="OO568_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6641506.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALEEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13533..14099
                     /gene="nrfB"
                     /locus_tag="OO568_002163"
     CDS             13533..14099
                     /gene="nrfB"
                     /locus_tag="OO568_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6641507.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14096..14767
                     /gene="nrfC"
                     /locus_tag="OO568_002164"
     CDS             14096..14767
                     /gene="nrfC"
                     /locus_tag="OO568_002164"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6641508.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCEHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14764..15720
                     /gene="nrfD"
                     /locus_tag="OO568_002165"
     CDS             14764..15720
                     /gene="nrfD"
                     /locus_tag="OO568_002165"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6641509.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEYDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAVCGASLVGVLMLRFFILYAGQLTVA"
     gene            15740..17458
                     /locus_tag="OO568_002166"
     CDS             15740..17458
                     /locus_tag="OO568_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6641510.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLAAFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17451..17834
                     /gene="nrfF"
                     /locus_tag="OO568_002167"
     CDS             17451..17834
                     /gene="nrfF"
                     /locus_tag="OO568_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6641511.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWKFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17831..18427
                     /gene="nrfG"
                     /locus_tag="OO568_002168"
     CDS             17831..18427
                     /gene="nrfG"
                     /locus_tag="OO568_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6641512.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18769..20082
                     /gene="gltP"
                     /locus_tag="OO568_002169"
     CDS             18769..20082
                     /gene="gltP"
                     /locus_tag="OO568_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6641513.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWSLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(20160..20849)
                     /locus_tag="OO568_002170"
     CDS             complement(20160..20849)
                     /locus_tag="OO568_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6641514.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDQRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20943..23090)
                     /gene="fdhF"
                     /locus_tag="OO568_002171"
                     /pseudo
     CDS             complement(20943..23090)
                     /gene="fdhF"
                     /locus_tag="OO568_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(23288..24754)
                     /gene="mdtP"
                     /locus_tag="OO568_002172"
     CDS             complement(23288..24754)
                     /gene="mdtP"
                     /locus_tag="OO568_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6641515.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24751..26802)
                     /gene="mdtO"
                     /locus_tag="OO568_002173"
                     /pseudo
     CDS             complement(24751..26802)
                     /gene="mdtO"
                     /locus_tag="OO568_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26802..27833)
                     /gene="mdtN"
                     /locus_tag="OO568_002174"
     CDS             complement(26802..27833)
                     /gene="mdtN"
                     /locus_tag="OO568_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6641516.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGDDSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAALEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(27852..28127)
                     /locus_tag="OO568_002175"
     CDS             complement(27852..28127)
                     /locus_tag="OO568_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641517.1"
                     /translation="MPTVLSRMAIHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(28336..>28443)
                     /locus_tag="OO568_002176"
     CDS             complement(28336..>28443)
                     /locus_tag="OO568_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641518.1"
                     /translation="VKAKRAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         6480 a         7201 c         7640 g         7122 t
ORIGIN      
        1 taccgcgata cgcctttttt ctctggctat aaaatgacct atatgcagcg gggaaattat
       61 gtggcagtta tcaaccctct cttctagagt gaagtgatgt ctgatgaccc gacattgcaa
      121 tggggtgtgt atgatacggt gacgaaaacc tttttctcgt taagcaaaga ggcctcggca
      181 gcaacgtttt ctccgctgat tcatttgaag gatttaaccg tacaaagaaa tggctattta
      241 tatgcgacaa tttattcgac aaaacgccca attgcagcca ttgttgcgac ttcatatcaa
      301 cgtcttataa cccattttta taatcatctt atttttgcgt tgcccgccgg tattttgggg
      361 agtcttgttc tgctattact ctggctacgt attcgacaaa actatttatc tcccaaacgt
      421 aaattgcaac gcgccctcga aaaacatcaa ctttgtcttt attaccagcc aataatcgat
      481 atcaaaacag aaaaatgtat cggcgctgaa gcgttgttac gttggcctgg tgagcagggg
      541 caaataatga atccggcaga gtttattccg ctggcagaaa aggaggggat gatagaacag
      601 ataactgatt atgttattga taatgtcttc cgcgatctgg gcgcgtacct ggcaacacat
      661 gccgatcgct atgtttctat taacctgtcg gcctccgatt ttcatacgtc acggttgata
      721 gcgcgaatca atcagaaaac agagcaatac gcggtgcgtc cgcagcaaat taaatttgaa
      781 gtgactgagc atgcatttct tgatgtcgaa aaaatgacgc cgattattct ggctttccgc
      841 caggctggtt acgaagtggc aattgatgat tttggtattg gctactctaa cttgcataac
      901 cttaaatcat tgaatgtcga tattttgaaa atcgataaat cgtttgttga aacgctgacc
      961 acccacaaaa ccagtcattt gattgcggaa cacatcatcg agctggcgca cagcctgggg
     1021 ttaaaaacga tcgctgaagg cgtcgaaact gaggagcagg ttaactggct gcgcaaacgc
     1081 ggcgtgcgct attgccaggg atggtttttt gcgaaggcga tgccgccgca ggtgtttatg
     1141 caatggatgg agcaattacc cgcgcgggag ttaacgcgcg ggcaataaaa ctacaggcgg
     1201 tggcgataat cgctgggagt gcgatcaaac tgccgacgga aaacgcggga gaaggtctgc
     1261 tgcgagacat aacccaggtc cattgcgata tcaaaaatcg gacgctcggt ggtgcgcaac
     1321 tcaacggcgg ccagtaacag gcggcgttgg cgaatgtaat cgccaagcgt ctgatgcgtc
     1381 accgtgcgga acatccgttg caagtaccac tttgaatagc ctgatttttt tgcgacgaca
     1441 tcaatgttaa gcggctggtc aatatgcacg tcaatccatg cgataagatc ctgaataatt
     1501 ttctgatggg acataaatct gcctcttttc agtgttcagt tcgttaattc atctgttggg
     1561 gagtataatt cctcaagtta acttgaggta aagcgattta tggaaaagaa attaccccgc
     1621 attaaagcgc tgctaacccc tggcgaagtg gcgaaacgca gcggtgtggc ggtatcggcg
     1681 ctgcatttct atgaaagcaa agggttgatt actagtatcc gtaacagcgg caatcagcgg
     1741 cgatataaac gtgatgtgtt gcgatatgtc gcaattatca aaatcgctca gcgtattggc
     1801 attccgctgg cgaccattgg tgaagcgttt ggcgtgttgc cagaagggca tacgttaagt
     1861 gcgaaagagt ggaaacagct ttcgtcccaa tggcgagaag agttggatcg gcgcattcat
     1921 accttagtgg cgctgcgtga cgaactggac ggatgtattg gttgtggctg cctttcgcgc
     1981 agtgattgcc cgttgcgtaa cccgggcgac cgcttaggag aagaaggtac cggcgcacgc
     2041 ttgctggaag atgaacaaaa ctaaagcgcc acaaggacaa gctgactcat cgttaactat
     2101 gaggccgggg tgagggtaaa tatttgcact ggtgctgcct gttcccctca ccctaaccct
     2161 ctccccgaag gggcgagggg actgatcgag ctcttttgga attatccgca aacacaaaac
     2221 taaagcgcca caagggcgct ttggtttgtt ttccggtctt tgtctttctc tctatcccgc
     2281 tggaacacag gagggtttcc cccgacgtca acacacctca ttcgagcacg tggtggaggt
     2341 tccggttggt gttgatgctt taattgtatg tcaccgacgt ttcttcgcca gtgtaaaagt
     2401 atacttttta gccgcaatat ttttgtcatc tcagacgatt ttttatcgca atccagaacg
     2461 gtatacggct cgataacgct gcaatcttgc acatcgacga taacgtttgc gcatcaattg
     2521 cctggttttt catcgtcaag acaataaaag agaaaaaagc agcaaacttc ggttgaaaaa
     2581 gccgctatga tcgccggata atcgtttgct tttttaccac ccgttttgta tgcgcggagc
     2641 taaacgtttg cttttttgcg acgcaccaaa ttgccgcaaa cctggagcag gaagataacg
     2701 tttcgctggc aggggattgt ccgccacgca tcttgacgaa aattaaactc tcaggggatg
     2761 ttttcttatg tctacgccat cagcgcgtac cggcggttca ctcgacgcct ggtttaaaat
     2821 ttcacaacgt ggaagcactg tccgtcagga agtggttgcc gggttaacaa cgtttctggc
     2881 gatggtctac tcggtcatcg tcgttccagg tatgttgggt aaagcaggct tcccgcctgc
     2941 ggcagttttc gttgcaacct gtctggttgc cggactcggt tctatcgtga tgggtctgtg
     3001 ggctaatctg ccgttggcga ttggttgcgc catctccctg acggcgttta ccgcattcag
     3061 cctggtgctg gggcaacata ttagcgtacc tgtcgcgctg ggtgccgtgt tcctgatggg
     3121 ggtgctgttt acggtaattt ctgccacggg tatccgtagc tggattttgc gcaacttgcc
     3181 gcacggtgtg gcgcacggca cggggatcgg tatcggtctg ttcctgctgc tcattgccgc
     3241 taatggtgtc ggtctggtga ttaaaaaccc gcttgatggt ctgcccgttg cgctgggtga
     3301 tttcgcgacc ttcccggtga ttatgtcact ggtaggtctg gcggtgatca tcggcttgga
     3361 aaaactgaaa gtccctggtg gcattctgct gaccattatc ggtatctcaa ttgtcggttt
     3421 gatcttcgat cctaacgtcc atttctccgg cgttttcgcc atgccttcat tgagcgatga
     3481 aaacggcaat tcactgattg gcagcctgga cattatgggc gcgctgaatc ctgtagtcct
     3541 gccaagcgtt ctggcgctgg tgatgacggc agtatttgat gccaccggaa ctatccgcgc
     3601 tgtcgccggc caggcgaacc tgctggataa agatgggcag attatcgacg gcgggaaagc
     3661 actgaccact gactccatga gcagcgtttt ctctggcctg gtgggtgcag ctccggcagc
     3721 ggtatacatc gagtctgcgg cgggtacggc ggcgggcggt aaaaccgggt tgacggctat
     3781 caccgttggc gtgctgttcc tgctgattct gttcctctct ccgctttctt acctcgttcc
     3841 ggggtatgca acggctccgg cgctgatgta cgttggcctg ctgatgctga gcaacgtggc
     3901 gaaaatcgac tttgctgatt ttgttgatgc gatggcgggt ctggttacgg cggtattcat
     3961 cgtgctgacc tgtaacatcg taacaggcat catgatcggc ttcgcgactc tggtgattgg
     4021 tcgtctggtt tccggtgaat ggcgcaagtt gaacatcggt acggtcgtta tcgccgtggc
     4081 gctggtgacc ttctatgcgg gtggctgggc tatctaatct ttcatcggat tctgaaaacg
     4141 ggtggcaatg gctgcccgtt tttattttct ccgcacattg tgtgagcttt tgcgatattc
     4201 tgaaaaaatg agaattcagg cataacgtct ttctcagatc ataaaaaaca tcgcaaacag
     4261 ggaacgcatg gaaatcttct tcaccatact gataatgacc ctcgtggtct cgctgtccgg
     4321 ggtggtcact cgtgtcatgc cctttcagat cccgcttccg cttatgcaaa tcgccatcgg
     4381 tgcgctactg gcgtggccga cgtttggttt gcatgtggag tttgatcctg aactcttttt
     4441 agtcttgttt atcccgccgt tgctgttcgc tgatggctgg aaaacgccga cccgtgaatt
     4501 tctcgaacat ggtcgagaga ttttcggcct cgcgctggcg ctggtggtgg tcaccgtggt
     4561 cggcattggc ttccttattt actgggtggt gccgggcatt ccgctgatcc ccgcctttgc
     4621 gctggcggcg gtactttctc cgaccgatgc tgtggcgctc tccgggattg ttggcgaagg
     4681 gcgcatcccg aaaaaaatca tgggcatttt gcagggcgaa gcgttgatga atgacgcttc
     4741 cggcctggtg tcgttgaagt ttgccgtggc agtggcgatg gggacgatga tcttcactgt
     4801 cggcggtgcg acggtcgaat ttatgaaagt agccattggc ggtattcttg ccggttttgt
     4861 ggtgagctgg ctgtatggtc gctcgctgcg attcctcagc cgctggggcg gtgatgaacc
     4921 cgcgacgcag atcgttctgc tgttcttgct gccattcgct tcttatctga ttgccgaaca
     4981 tattggcgtt tcgggcatcc tcgctgcggt tgccgccggg atgaccatca cccgctccgg
     5041 tgtgatgcgc cgtgcgccac tggcaatgcg cctgcgcgca aacagcacct gggcgatgct
     5101 ggaatttgtc tttaacggca tggtgttcct gctgttaggt ctgcaactgc cgggtattct
     5161 ggagacgtcg ctgatggcgg cagaaatcga ccctaacgtc gaaatctgga tgctgtttac
     5221 cgatattatt ctgatatatg cggcgctgat gctggtccgt ttcggctggc tgtggacgat
     5281 gaaaaagttc agcaaccgct tcctgaagaa gaagccgatg gagtttggtt cgtggaccac
     5341 acgagaaatc ctgatcgcgt ctttcgctgg ggtgcgtggg gcgatcactc tggccggtgt
     5401 gctctcaatc ccgctgctct tgccggatgg taacgtcttc ccggcgcgct atgagctggt
     5461 gttcctggcg gctggcgtca ttctcttctc gctgtttgtc ggcgtggtga tgttgcctat
     5521 tctgctacaa cacattgaag tcgcggacca ttcgcaacaa ttgaaagaag aacgtattgc
     5581 gcgagcggca acggcagaag tggcgattgt ggcgatccag aaaatggagg agcgtctggc
     5641 ggcggatacc gaagagaata tcgataacca gttgcttacg gaggtcagtt ctcgcgtcat
     5701 tggtaacctg cgtcgtcgcg ccgatggacg taacgacgtt gaaagttccg tgcaggaaga
     5761 gaaccttgag cgtcgcttcc gtctggcggc attgcgttct gaacgtgctg aactgtacca
     5821 cctgcgcgcc acgcgggaga tcagcaacga aacgctgcaa aaattactgc acgatctcga
     5881 tttgcttgaa gcgttgctaa ttgaggaaaa tcagtaagcc gaaagcgacg taaccaggcc
     5941 cgaggtaatc tgaaaaaggt gcaatctttt gcacctttaa caggttagct cgggcgaaca
     6001 ttcatgattc taaaacaaat aagttatctc tcttgtaaag tcaattcatt ggctgttctt
     6061 cgacttaacc tcaatctgcg tgttgcataa tatctgcagt aatcttcata gcgactgcat
     6121 taatattatc ctcagtttgt gacatgccag tatgagaggt acaatcttct tcaaagaatt
     6181 tctgtagtgt taccagggta tcactatcaa tggggatatt ttcctcccca cggagaatgg
     6241 aaatgcccat tccctggacg ctagcctcat cgaaattaac ccgatagaga cgggtattaa
     6301 acatacaggc ttttgttaaa atagaacagt tgaaatggca gttgcttaaa ttggcattat
     6361 taaaattaac attagccata ttagagccgg tacatataac catttcgagg aatgtatttg
     6421 tcagccacgt acaaaagagt ttgctatcaa ttaagtttat attctggagg attgctttgt
     6481 tcagattgac gcgagacaga tcagcatgtt caaagttaat taaatcgagt ttagagaaac
     6541 taaggtcggc tttaaataaa ttaacatatg gcgctattac tttataaaga tttacctcag
     6601 ccatgtacgc catataaaaa tttgaatagt caaggaaggt atttttaata ttggctctat
     6661 tcattgctgc attgtagaat tgaacatgca tagcggagca tgcatttaga gttccgtcgg
     6721 ccatttttat ctgagcgaaa tttgagtcat cgagaataca attatcaaaa agtatttttt
     6781 ccattatggc gtaataaaaa ttcgttttat acatattaca gtcctggaaa atagcgtttt
     6841 gtagtctgca acctgcaaaa ttcgaattgg ataaatcttc atctttaatc acgataccct
     6901 tcaggctaag gtgtgaaaaa tcgtaaccac catttactat ctgtggaatt tcgatagtat
     6961 tattatggac ggcgacatta tttataaaat ataccacctt aatggataca tccttttctt
     7021 tactaaggta aactcttctc gtaattctat cgtcacattt attatttgca tataacgcta
     7081 tatctataaa atatttatgt attttcctgt ccgtttcttt cgtgattttt cctgatggta
     7141 aactatcata aattcttttc attttctgaa tcaagcctat cactttttca tgatcatcta
     7201 ttttgaaaag agagagggct ttattgtcaa ttaacgtgtt tttatgatgt ttgctgtaat
     7261 tatctgattt tgttttcttt gtatgtgatg aacttgtgat agagtggtta tcgttaatct
     7321 ggcaaagagg gaaaaacata ttatttaaac cattatagcg cataaaatat ccttataatt
     7381 aacaagaaag aaaaggcata ttctctgcat tatcattttc tacgactgtc aaaaatcgct
     7441 cattttttaa tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat gttggagagg
     7501 aagagtattc cccggtcagg cgaccgggga aggggagaga ttaatgtgcg cggccttgct
     7561 caacgccaaa gccggtctgg gagcggataa actgcgcgcg gaacagttca cgctcgcgcg
     7621 ccccttccgc tgagttatcg gttgccgaga agaaccagat gccgaggaat gccacgctga
     7681 tagagaacag cgccgggtat tcatacggga agatggcttt ttcgtgaccg aggatctgta
     7741 cccaaatagt cgggccgagg atcatcagca ccactgcggt aatcagcctc agccagccgc
     7801 ccagcatcgc gccacgggta gtcagtttcg accagtacat ggaaagcaga atgatcggga
     7861 agttacagct cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg ttctgattct
     7921 caaacagcac gccgaggata atcgcaatca cgccgaggat cagtacggtg atttttgata
     7981 cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac aagtcatgcg
     8041 aaaccgccga tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata gtggcgaaag
     8101 caaccgctga aataaaaccg aggaataggt tgccgcccac tgcattcgcc aggtgaacgg
     8161 ccgccatgtt gttaccacca atcagatggc ccgccgcgtc tttatattcc ggattcgcac
     8221 caacaagcat gatcgcgccg aagccgataa taaaggtcag aatatagaag tagcccataa
     8281 atccggtggc gtagaacacg ctcttacgtg cttcgcgggc atcgctgact gtaaagaagc
     8341 gcatcagaat gtgcggcaag cccgccgtac caaacatcag tcccagcccc agagagagcg
     8401 cggagatcgg atctttcacc agtccgcccg gtttcatgat gtcgacacct ttcgggtgta
     8461 ccgccatcgc ttcgctgaac agattgttga agctaaagcc gacgtgtttc atcaccataa
     8521 aggccataaa gctggcaccg aacagcaaca gcacagcttt gataatttgt acccaggtgg
     8581 tcgccagcat gccgccgaac aggacgtaca tcatcatcag cacgccgacc agcatcaccg
     8641 caatgtgata gttaaggcca aacagcagct cgatcagttt acctgcgcct accatctgag
     8701 cgataaggta aagcgccacc accaccagag aaccacaggc cgaaagaata cgaatcggcc
     8761 cttgtttcag acgataagag gccacatcgg cagaggtgta gcgtcccagg ttacgcagac
     8821 gttcggtaat cagaaacaga atgattggcc agcccaccag gaagcccaac gagtagatca
     8881 gcccgtcata gccggaggta aacaccagcg cggagatccc caagaatgag gcggcggaca
     8941 tatagtcccc ggcaatcgcc agcccgttct ggaagccagt gatattgccg cctgcggtgt
     9001 agtagtcgct acgagaacgt acgcgttttg atgcccagta ggtaatgccg agcgtaaaca
     9061 cgacgaaaat caggaacata ataatcgcct gccagttcgt tggctggcgc tctacggccc
     9121 cgctaatagc atccgcggcg ttagctgcga aagggagtgt ggcggcaagc gccgtcagaa
     9181 ctcttttcat gatgcttgta cctcatgcag gacttcatta ttaagacggt cgaattcgcc
     9241 gttcgcccgc cagatgtaga taccggtgag aacaaaggag atcacaatca ctccaacacc
     9301 aattggaata ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca gccagccggg
     9361 cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc agcataataa tcgacaggat
     9421 ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt cttctatccg
     9481 ctgataaatc gtaccattca tcacagattc tccagaggta atgtagggat tgttttaatt
     9541 cccgctccct tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg taggccggat
     9601 aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg acgctgtcgc gtcttatcag
     9661 gcctacaaac cgttaccgat tcgcatccgg caattgtggg ttacgatggc atcgcgatag
     9721 cctgcttctc ttcaagcagc ttctcgacca cgccaggatc ggcaagcgtc gaggtatcgc
     9781 ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat acggcgcata attttgccgg
     9841 agcgggtttt aggcagggag tcggtccagt gcagcacgtc tggcgtcgcc agcgggccaa
     9901 tctctttacg cacccagttg cggacttctg cgtacagttc tggtgacggt tcctccccgt
     9961 gattaagcgt gacataggcg tagatcgcct gacctttaat attgtgcgga atacctacta
    10021 cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga ctctatctct gccgtcccca
    10081 gacggtgacc ggagacgttc agcacgtcgt ccacacgccc ggtgatccag taatagccat
    10141 cttcatcgcg acgcgcgccg tcgccgctga aatacatatt tttgaaggtg gaaaaatagg
    10201 tctgctcaaa acgttcgtga tcgccaaaca gcgtacgcgc ctgaccaggc caggagtcgg
    10261 tgatcaccag gctaccttcg gtagcccctt ccagcgggtt accttcgtta tcgaccagcg
    10321 ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc tttcagctcg gtagcgccag
    10381 gcagcggggt gatcatgaaa ccgccggttt cggtctgcca ccaggtatcg accaccggac
    10441 atttctcgtt gccgattttt ttccagtacc actcccacgc ttccgggtta attggctcgc
    10501 ccacggaacc gagaatgcgt agcgacgaac ggtcggtgcc ttcaatcgct ttatcgcctt
    10561 ccgccatcag cgcgcggatc gccgtgggtg cggtatagag aatattgacc tgatgcttgt
    10621 ccaccacctg cgccatacgg gcaggcgtcg gccagttcgg tacgccttca aacatcagcg
    10681 tggtcgcgcc gcaggccagc gggccgtaca gcaaataact gtgtccggta acccagccca
    10741 catcggcggt gcaccagtag atatcacccg gatgataatc aaagacatat ttaaaggtca
    10801 gcgccgcgta caccagataa ccgccggtag tgtgcagtac gccttttggt tttccggtag
    10861 aaccggaggt atagagaata aacagcggat cttcggcgtt catctcttcc gcctggtgct
    10921 gatcgctggc ttgctcaacc aggtcgtgcc accacaggtc gcgcccttcc tgccagtcga
    10981 tgtttccgcc agtacgcttc agtaccacca catgctctac gctggtgacg ttcgggtttt
    11041 ttagtgcgtc atcaacgttt ttcttcagcg gaatactacg cccggcgcgc acgccttcgt
    11101 cggaagtgat caccagtcgt gagttggaat caataatgcg cccggcaacg gctttcggcg
    11161 agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg ggcgcaggcc agcatcgcaa
    11221 ccgcggcttc cggcaccatc ggcatataaa tcgccaccac atcacctttt ttaatgccca
    11281 gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg cagctcttta tagctgatat
    11341 gtttgctctg gctggcgtcg tcgccttccc agatgatggc ggtgcgatcg ccgttttctt
    11401 gcagatggcg gtcaaggcag tttgccgcca gattcagcgt gccgtcctcg taccatttaa
    11461 tggacacatt accgggggca aaggaggtat ttttcacctt ctggtacggt ttgatccagt
    11521 cgagaatttt tccctgttcg ccccagaagg tatcaggtac gttaatagat tgttgataca
    11581 tcgcctcgta ctgctgaggg tttatcaggc aacggtctgt gatgttggca ggaatggtgt
    11641 gtttgtgaat ttggctcatg cttttgttct ccttgtagga tgttaataat atgtggcata
    11701 agcgttaaat gtaggggtat tggcagtttt gtttagtatt tgggcgacag atcacgcaaa
    11761 agtagaatgg tgcaaataaa cggcagggta atttttgaag gtcaccaaca aaagttgatt
    11821 aattctttga ggaacatgca gttatgcatg ctgttgaaaa agaggaagat actaactaac
    11881 tctaaagtgg tattttacat gcacttacaa ttgattaaag acaacattca cagtgtggtt
    11941 atttgttaca cataggggtg agcaatgtca tgacagtgta ggtgcggtta ctgtcgtgaa
    12001 aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg atgaagcccc tatgacaagg
    12061 ataaaaataa acgcacgccg tatcttcagc ttattgattc cttttttctt tttcacttct
    12121 gttcacgctg aacaaacggc agctcccgca aaacctgtaa ctgtggaagc gaagaatgaa
    12181 acctttgccc cgcagcatca cgatcaatat ctctcctgga aagccacctc ggaacagtca
    12241 gagcgtgttg acgccctgga ggaagatcca cggctggtga tcctgtgggc ggggtatccc
    12301 ttctcgcgcg attacaacaa gccgcgtgga catgcctttg ctgtgaccga tgtgcgtgaa
    12361 accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat ggcgtgctgg
    12421 agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag acggcgaaga tggctacttc
    12481 cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg tgccgactgc
    12541 cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct ttcccgtccg
    12601 tatgcggctc gcgcgatgga agccattggt aaaccttttg agaaagccgg acgtttcgac
    12661 cagcaatcga tggtttgcgg tcagtgccat gtggagtatt acttcgacgg caaaaacaaa
    12721 gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa atatggagca gtattacgac
    12781 aaaattgcct tctctgactg gactaactcc ctgtcgaaaa cgccaatgct gaaagcgcag
    12841 cacccggaat atgaaacctg gacagcgggc attcacggta aaaacaacgt gacctgtatc
    12901 gactgccata tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga ccataaaatt
    12961 ggtaatccgt ttgataactt cgcccagact tgtgcgaact gccataccca ggataaagct
    13021 gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta acgacctgaa aatcaaggtt
    13081 gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg cgctggatgc aggcgcgacg
    13141 gaagccgaaa tgaagccgat tcaggacgat atccgtcatg cccagtggcg ttgggatctg
    13201 gcgatcgctt cccacggtat tcatatgcac gcaccggaag aaggtctacg gatgctcggt
    13261 acggcgatgg ataaagcggc ggatgcgcgc accaaactgg cgcgcctgct ggcgaccaaa
    13321 ggcatcaccc atgaaatcca gatcccggat atctcgacca aagagaaagc ccagcaggcc
    13381 attggcctga acatggaaca aatcaaggcc gagaagcagg acttcatcaa aacggtgatc
    13441 ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa gccaataacc ccgttccgcc
    13501 tcgcaagggg cggaaaacac aatggagtga atatgagcgt attacgttcg ttattaactg
    13561 ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct acccctgcgg
    13621 tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg taacccggat gccgcctgtc
    13681 tggactgtca taaaccagat accgaaggta tgcatggcaa acatgcttcc gttatcaacc
    13741 cgaataacaa attgccggtc acctgcacca actgtcacgg ccagccatca ccgcaacacc
    13801 gcgaaggggt gaaagatgtg atgcgcttta acgagccgat gtacaaggtc ggagagcaga
    13861 acagcgtctg tatgtcctgt catctgccgg aacagttgca aaaagcgttc tggccgcacg
    13921 atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca cagcaagata
    13981 cgatgcagac gttaagtgac aaaggacgga ttaagatttg cgtcgattgc cacagcgatc
    14041 agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt gcttaaggag cagccatgac
    14101 ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg gcagccgtca gcggcaccgc
    14161 agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg cgttacggca tggtgcatga
    14221 cgagtcgtta tgcatcggct gtaccgcctg tatggatgct tgtcgggaag tgaacaaggt
    14281 gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg aatttcctga
    14341 tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc gagcatgcgc cgtgcgttga
    14401 cgtctgcccg accggtgcgt cttttcgtga tgccgctagc gggattgtcg atgttaatcc
    14461 tgatctctgc gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg tgcgctttat
    14521 ccatccggtc acgaagacgg cggacaaatg cgatttctgt cgtaagacca atttgcaggc
    14581 cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag gcgctgacgt ttggcaatct
    14641 ggacgatcct aacagtgaga tttcgcaact gctgcgccag aagcctactt accgctacaa
    14701 gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg gggaggtgag
    14761 ccaatgacgc agacttccgc atttcatttt gaatcgctgg tgtgggactg gccgattgcc
    14821 atctacctgt ttttgattgg tatttctgcc ggtctggtga cgctggccgt gctgttacgt
    14881 cgcttctacc cgcaggcggg cggtgcagac agtacgttgc tgcgcaccac gctgattgtc
    14941 gggccgggcg cggtgatcct cggtctgttg atcctcgtct tccacctgac aagaccgtgg
    15001 accttctgga agctgatgtt ccactacagt tttacctcgg tgatgtcgat gggggtgatg
    15061 ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg cgaaaatctt tgaatatgat
    15121 ttgcttgccc tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa ggttctgagc
    15181 cttctgacgc ccgttcatcg cggactggaa acgctgatgc tggtgttggc ggtgctgctg
    15241 ggggcttata ccggctttct gctgtcggcg ctgaaatcgt atccgttcct caataacccg
    15301 agcctgccgg tgctgctcct cttctccggc atctcgtccg gtgcggcggt ggcgctgatc
    15361 gccatggcga tacgccaacg cagtaacccg cattccacgg aagcgcactt tgttcaccgg
    15421 atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg tggcgttttt tgtcggtctg
    15481 gcgctgggcg atgacggtaa agttcgtgcg ctggtggcgg cattaggtgg cggtttctgg
    15541 acgtggtggt tctggcttgg tgtcgccggg ctggggctga ttgtgccaat gttgctcaaa
    15601 ccgtgggtca atcgcagttc cggcattcct gccgtgctgg cggtgtgtgg ggccagtctg
    15661 gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg gacagttgac ggtggcgtaa
    15721 gccagaaaag aggtggtttc tggacgtatt ccttcctgaa gtcggttttc tggcgttgtt
    15781 gttaagtctc ggggtcaacg tgttgacccc gttgacggcc tttgcgggag tgcggttgcg
    15841 ctggcctgcc atgatgcgac tcacttgcat cggcattctg gcgcagttcg cgctcctgct
    15901 gctcgccttt ggcgtactga cgtattgttt tctcatcagc gatttctcgg tcatttatgt
    15961 cgcccaacat agctacagcc tgctgtcgtg ggaacttaaa ctggcggcgg tgtggggcgg
    16021 tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc gcctggagcg cgctgtttgc
    16081 ctggcattat cggcagcaaa gcgatccgct atttccgctg acgctagccg ttttatctct
    16141 catgctcgcc gcactgctac tgtttgtggt gctgtggtcc gatcccttcg tgcggatatt
    16201 tccaccagca atcgaaggcc gcgatctcaa tccgatgctg caacatcccg gtcttatctt
    16261 tcatccaccg ctgctttacc ttggctatgg cggtttgatg gtagcggcga gcgtggcgct
    16321 ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc cgaatttgct ggcgctgggc
    16381 gttacctggc tggagtgcat taacggcggg gatcatcctc ggttcctggt gggcctactg
    16441 cgaactcggc tggggcggct ggtggttctg ggatccggtg gaaaacgcct ctttattacc
    16501 ctggctttct gccactgcgc tgctgcacag tttgtccctg acacgccagc gggggatttt
    16561 ccgccactgg tcgctgttgc tggcgatagt tactctgatg ctgtcgctgc tgggcacctt
    16621 aattgtccgt tctggcattc tggtttcggt tcatgcgttc gcactggata acgtccgcgc
    16681 cgtgccgttg ttcagcctgt ttgcactgat tagccttgcg tctttggctc tgtatggctg
    16741 gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg ttatcgcggg aaatgttaat
    16801 cctcgctacg ctgttgctgt tttgcgcagt gctactgatc gtgctggtgg gaacgcttta
    16861 tccgatgatt tacggcctgc tgggctgggg acgcctctcc gttggcgcgc cgtattttaa
    16921 ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg attgtgctgg cggcgtttgt
    16981 ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat gctggcgtgc tgttatttgc
    17041 cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc agcctgaatt tacagccggg
    17101 tcagcaggtg acgctggcag gatacacctt ccgttttgag cgactcgatc tgcaagccag
    17161 aggcaattac accagcgaaa aagcgatagt ggcgctgttt gaccatcagc aacgcattgg
    17221 tgaattaacg ccggagcggc gtttttatga agcacgccgt cagcaaatga tggaaccgtc
    17281 aattcgctgg aacggcatcc atgactggta tgcggtcatg ggtgaaaaaa ccagagcgga
    17341 tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg ggggaggatt
    17401 gttgatgatt gcgggcgcat tgttaagcgg atggcggggg aagaagcgcg atgaataaag
    17461 ggcttctcac gctactgctg ttatttacct gttttgcgcg cgctcaggtt gtagacacct
    17521 ggaaattcgc caatccgcaa caacagcaac aggcgttaaa tattgccagc cagttacgtt
    17581 gtccgcagtg ccagaatcaa aacttactcg aatccaatgc gccggtggcg gtcagtatgc
    17641 gccatcaggt ttatagcatg gtgtcggagg ggaagaacga agtcgaaatc attggctgga
    17701 tgaccgaacg ctacggagat tttgttcgct ataacccacc gttaacgggt cagacgctgg
    17761 tgttgtgggc gctgccagtg gtgttgttat tgctgatggc actgatcctc tggcgagtga
    17821 gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg ctgacaacac tcacgatatt
    17881 gatggtattc ctctgtgtcg gcagttatct gttaagccca aaatggcagg cggtacgtgc
    17941 ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc agccagcaaa ccccggaagc
    18001 gcagcttcag gcattgcagg ataaaatccg cgctaatcca caaaacagcg aacaatgggc
    18061 gttactgggc gagtactatc tgtggcaaaa cgattacagc aattcgctgc tggcgtatcg
    18121 tcaggcgttg caactacgtg gtgagaacgc tgaactgtat gcggcgctgg cgacggtgct
    18181 ttattaccag gccagccagc atatgaccgc ccagactcgc gcaatgatcg acaaagccct
    18241 cgcgctggac tctaacgaaa tcaccgctct gatgctgctg gcttccgatg cgtttatgca
    18301 ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg atggatctca actcaccccg
    18361 gatcaaccgg acacagctgg tcgagtcgat taatatggca aaactgttgc agcggagatc
    18421 ggattaatca tctggtgatg aagattatgt cattgagctg cataaaaaat aatcgaatga
    18481 acatatgcca aaaataatca ccaatcagta ttattgcaga ttaaacaaat aaaaatcttt
    18541 ccataacaaa tggttattca ttaatcctgc cataaactcg cgttattatg cattaatgca
    18601 gcgaaaagct ctgttgttaa agggttgcgc aacataccgc gcaaatgata ctgatcataa
    18661 gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg ccctggcagt atgtcacgtt
    18721 ctcgcgtttc tgaacgggga acggcgctcc attgaggaag ttattctgat gaaaaatata
    18781 aaattcagcc tggcctggca gattctgttt gctatggtgc tgggcattct cctgggaagc
    18841 tacctgcact accatagcga cagccgcgac tggctggtcg tcaatttgct ctctccggcg
    18901 ggtgatatct tcatccatct gatcaaaatg attgttgtgc cgattgtgat ctccacgctg
    18961 gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg cgcgaaaacc
    19021 attatttact tcgaggtgat caccaccgtc gccatcattt tggggatcac tctggcgaac
    19081 gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga tatctcgaaa
    19141 tatcagagca ctacggaagc ggtacaaagc agttcccacg gcattatggg cacgattttg
    19201 tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct gccgatcatc
    19261 tttttctcgg tgctgtttgg tctggggctt tcttccctgc ccgcgacgca tcgtgaaccg
    19321 ctggtgaccg tgttccgctc catctctgaa accatgttta aagtgactca catggtgatg
    19381 cgttatgcac cggtgggtgt gtttgcgctg attgcggtga cggtggctaa ctttggtttc
    19441 tcgtctctgt ggccactggc gaaactggtg ctgctggtgc atttcgccat tctgttcttc
    19501 gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt taagcgtctg gagcctgatt
    19561 cgtattctga aagatgagct gattctggcg tactccactg ccagctctga aagcgtactg
    19621 ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac cagcttcgtg
    19681 gtgccgaccg gttactcttt taaccttgat ggttcgacgc tgtatcaaag tattgccgct
    19741 attttcatcg ctcagctgta tggcattgac ctgtccatct ggcaggaaat cattctggtg
    19801 ctgacgctga tggtgacctc gaaagggatt gctggcgtgg ctggcgtgtc gtttgtggtg
    19861 ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag gtctggcgtt tattgctggt
    19921 gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa tgcgctggcg
    19981 gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct ggcttatgag
    20041 cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat aattgaagat tgccggggat
    20101 atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc gcgtcttatc aggcctacct
    20161 taaccgttgg ttaatttttc aaactcttca cacccggtat caaacccttc catacagctc
    20221 aggttcaacc agtgcagcgc cttttgtttg ttcttctcga taaaaccttc ctcaccgttt
    20281 aagaacatca ttcccgccca gtactcggaa taaccggttc gggaaattgc agagctgcgt
    20341 ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt ggcgtaaatc
    20401 aaaccaagca gcatttgggc atcgacggcg gagtcattct ccagatcttc cgaggcgttt
    20461 tctaacagcg aaatcgcctt tggataatcc ggtttacccg cttgagtatt taccagaata
    20521 tgcgccagcg ttacttcacc ttctttactg cccgcttgcg ccgctttctc tgcaagaact
    20581 tttgcttgtg gatagtccag actgaccgga ttggtgattt tgatctgcgc caacagcgcg
    20641 caggcatcag catcaccgct gtcggcggct ttctgtgccc aatattcggc tttgctcaaa
    20701 tcgccggagc taaaccagct gtcggcaaga aaatattgtg cgcgttggtc cccggcctct
    20761 gctgccttta aatactggct gccaggctcg gagtcgttcg cgtgggcaaa gaatgtcaaa
    20821 aacaacatca atgcgataat ttttttcatt ttgataatac gattgggtag ggagtaacca
    20881 gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta gaaaggactg
    20941 tattacgcca gtgccgcttc gcgcaggcga gttttcaact tgttgtactc gtcaatcagg
    21001 tactgctcgg cggcgcgctg atcggagatc ggctcgacgc gcacggcgca gtatttgtat
    21061 tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca ggcaccaatc
    21121 caccactggt aggtcatgta aatcgcccct ttgttcggac gatcgctgac ctgcgcacgg
    21181 gtgataattt tgcctttacg cgagtgcact caaaccaatg cctcatcttc aatacccaga
    21241 cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt catcagccag cgccgccagt
    21301 gccgcacagt taccggtcat cgaacggcaa gagtagtgac caacttcacg caccgttgac
    21361 agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc gcaggtgaag
    21421 aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa acagataaga agtcccctga
    21481 tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc cattttctcg
    21541 taagtcgcac cgtagaaatc cgggcacaga tgacgcaact catcccagat ctcctgggtg
    21601 ttgttgtagt gcatcggata acccatacgg gtggcgattt cactgatgat ttgccagtcc
    21661 gttttcagat cccatttcgg ttcaactgct ttgaagaaac gctggaagcc acggtcagcc
    21721 gcagtaaaca cgccttcatg ctcgccccac gacgttgacg gcaaaatgac atccgccgcc
    21781 gacgcggttt tggtcataaa gatgtcctga acgatgacca gttccagatc ttcaaaggct
    21841 ttacgtactg ccgacagctc cgcatcagtt tgcagcggat cttcgcccat aatgtacgcg
    21901 gcacgcactt cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata gccggtatga
    21961 gcaggcaggc tttccacgcc ccaggctttg gcgaatttct cgcggttagc cggatctttc
    22021 acgtactggt atcccggata cgtatccggc agcgcgccca tatcgcaggc accctgcacg
    22081 ttgttctgac cacgaaccgg gtttacaccc gcatgcggct taccgaggtt accggtcagc
    22141 atcgcgaggc tggtcagaga acgcacggtt tccacgccct ggtagaactg ggttacaccc
    22201 atgccccaca ggatggcggc gcttttcgcc tgggcataca tccgtgccgc ctgacgaatc
    22261 tcactggcgc tgacaccggt gatatcttca accgactccg gcgtgtagcc ttcaacgatt
    22321 ttacgatact cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc gtacagattt
    22381 tcttcaataa tgacatggcc catcgcattc aacagcgcga tgttcgagcc gtttttcagt
    22441 gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca gacgataatt
    22501 ttcgccccgt tacgtttagc gttaattacg tgattcgcca cgattgggtg ggaatccgcc
    22561 gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat agcattgctc
    22621 attgcgccat taccgaccga ttggtgcaga cctgcaaccg atgggccgtg tcagacacga
    22681 gcgcagcagt caacgttatt ggtaccaata acggcgcgcg caaatttttg cattacatag
    22741 ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct ggatggcatc cggaccgtac
    22801 ttctctttga tggcgctcag gcgctcggca acgtaattca gtgcctcatc tcaggaaaca
    22861 ggttcgagtt tgccgccacg ctggcgacgg atcatggggg ttttcaggcg cggggtcagg
    22921 atctgggtat cgttaatgaa atcccagcca taataaccct tcagacacag ggtaccctgg
    22981 ttggttttcc cctgcgctgc ctccgcccgg acgattttgc cgttatcgac gaccaggttg
    23041 attttgcaac ctgatgcgca ataggggcaa accgtgacga cttttttcat cggtctcgct
    23101 ccagttaatc aaatcacgca tacgcgctct cgactacagt atgcatcttt tatgccacat
    23161 tttatttggg gtcattccct gatattacgg gcactattta ttcaaaactc tgacgaaaaa
    23221 caggctgtcg tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc atcacgcggc
    23281 agacatttta ttttttctcg acgacgggag ccgcctgata cccgccgccc agcgatttca
    23341 tcaactgaat gctttggatc acccggcggc tgtccagcat cagtaatgac atctcttcgg
    23401 caagtactgg caaccgggct tcagtggcct gtaagcggct ggttaagccg cgctgatagg
    23461 cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac gtgttcagcc tgcatttctc
    23521 gctcgtcgtt gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc accgcgttca
    23581 gtactgactg gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg ccttcgagat
    23641 tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag acccgggatg aagttgaact
    23701 ggcgactggt ttttttgaat aaggtatcta ggtggatggc atcaaggccg aaaaacgctt
    23761 tgatatcaaa gctcggatag aacaacgccc gcgcggaatc cacctgatct aatgacgcct
    23821 gaacatacca gcgcatggct tgcagatccg ggcgtctggc gagcaactca taagagagtg
    23881 tcgccggaat gccggtctgg actcgtggta atgccacagg tttgatctcc ggcatatcgc
    23941 tggctcccgc gccaatcaat gcgcgcagag attctctcgt ttcggtgatt tgccctttga
    24001 cggcagcaat ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg aaaggcactt
    24061 gtgcttccag accgtgcgcc actttacttt ggtgcgcttt caccgcgtaa tcaatcacat
    24121 cgcgagtttg ttctaacaga tcgagcatct gatagctggc ctgcatactg taataaagct
    24181 gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt ttctgccagc gcggcattat
    24241 gcgcgccaat ggcggcggca accgctgagc gatgcacacc ccacaaatca agatccagtc
    24301 cggcaaacaa acctactgtg gcttccgtat agtacggccc gtccatcccc agtgcgggcg
    24361 catccatcgc ataagggctt aaaaagccgt tcgccgagac acgttggcgg ttaagcatcc
    24421 ctaacgctgc cacctgcaac tgtgaaccgg catctaacaa atcggcctgc gattgcgctt
    24481 tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc cgttggatca
    24541 gggcatccaa ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc cagccggagc
    24601 tggcaagatg aatatcatcg gccagtttga tttgttccgg tttgagctgt tgatgaggcg
    24661 cagaatcctt acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg agaatgctgc
    24721 acaacagcag acgtgaaagt tgacgattga tcattgcgtg gctccttgtg cctgttccgt
    24781 ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct ggcagacgtg
    24841 ttgctcctgt tttaataagg tgggcaggat ggcctgcgag gcgggtgtct cttcaagcgt
    24901 tatttgagga gaacggctga gtgcggttgc cagaccggca gcgtatttct ccagggcgtc
    24961 ggcaaactga cctgctctgt ccggctgtag tgcgttatcc agcgcctgcg ccgaattcca
    25021 ggtggcatcc caggcgtgaa ggatatcgcg gccctgacga ataaccgttt gcgaacgttc
    25081 aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc gttggcacat
    25141 ctcttcacag gcattaaacg ccgcatgcag accgatacga atttgcagat aagtgcgcag
    25201 agccgtgact tcctgctggc gtgggatccg cattacttta cttaacatac ccagcgcgcc
    25261 agccagtttt tgcggcagcg tgcgcgcttc actttcaggc cagacaaagg tgtaaatcat
    25321 cgcggacacc acggtaccaa tgatgatacc cagggcgcga tcgcgaattt ccaccaggtc
    25381 gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacca ccatctgtgt
    25441 gccgatatta gaagagcgtt cagagctggt ggcaatctat gcgcccaaca ggaaaatcgg
    25501 tgccagcaca aacagcaatt cgacaatatt gtccagccag ggcatgacca gtagcgtgaa
    25561 taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca tcttctggta
    25621 cgacgaaccg acatttggat tagcgacgat cacgcaggtc agcatacagg tgtgaatgcc
    25681 ttcccagtcc acgccgctgt aaaaggtgta acagatcaaa caggcgagca atgtttttac
    25741 cgcgtagcgt atatagtctg gattggtaaa agcatcggcg accattgatg gcggtttggc
    25801 ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt tgtaacaacg tctggcagat
    25861 attctccagg ttacattccc gtgccgccac cgcttcactt tcactgatcc gccagtcgct
    25921 ttgccagcac tgaccttcag caacggcatg ctgcagcttg ttgatttctg aggctaattt
    25981 ttgccgaaat tcaataattg cctgagaatc agcaaaagag gtgggatcgt agcgattcag
    26041 cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc caccatgcgc tttgagttcg
    26101 ccagttggca tcgtccgcga gacaaaagac attcagtttt tgcagcgcca gcgcctctct
    26161 ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc aggtggctaa tggcatcatc
    26221 aagccgatca ttaagcgctt gatgcatttg cgtaatggca cgattgggaa accacagcac
    26281 gccgattaac gtcatcagca aggttggata gaggccaaca acgatacacc acagcgttaa
    26341 gcgcacgact acttccggat agtcgagcat ggcggggaag gtttgcccgt aaatggcgac
    26401 aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg cgcatcaaaa acatgcagcc
    26461 catcaggatc ggcccggcga tgatcaatcg gatcaacggt tcgccgtatg accatttgta
    26521 gatcaaaaac aggctgccga tctccagcac cgtggcaacc acaaacaaga tcgccacaaa
    26581 tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc actgccagcg ataacgccac
    26641 aaaagggatc tcaaaagtca tcgagatcag aatcaccagc aggcagccta cccagagttg
    26701 cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac tcttcatgaa agaacgccag
    26761 cagcctgacc accggtaatg gcagggagtt gagcgcgctc attattgcgg ctcaagatta
    26821 gcgactgccg aagcgccgat gcggaacatt tccgggtcag gtttatcgac catgattttg
    26881 accggaaaac gctgggcaac gcggacccag ttaatagaac gagacacttt tggcaggccg
    26941 cccagcacca ggccgccgtc atccggtagc acgccgtagc caatcgaatc cactttaccc
    27001 tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg cgggtgtacc tgagcgaata
    27061 tttttcagat cggtttcgcg gaagttggcg atcacgtacc agtgacgagt gtcgatgagg
    27121 gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg aggttttgag ggaaatgatc
    27181 cggccatcaa atggcgcgcg aacggtcgtc atttccagat gtagtttggt cagggcaata
    27241 tccgcttcga gcgccgcacg ttgcgccacc agcgcatcga cgccgctgac ggcgctggcg
    27301 gctgactgcg cctgtaacaa tacggcatta agatccgctt ctgcggcgcg ctgcgccgtt
    27361 cttgcacggt caacgtcttc tgctgagaca aaaccctctt tcagtaatgg ctcggtgcgg
    27421 cgtaatgtat ctgtggcctg tttcgcggcg gcacgggctt tttctaccgt ggcattaacc
    27481 gagtcgtcac caaactgttg ggcgtcaacg ctacgctggg tgagcataat ttgcttatcc
    27541 agcgccgcga gggaggcttc ggctttcgcc aggttggctt cgtacgggcg cgggtcgatg
    27601 cggaacagca aatcgccctg tttcaccgcc tggttgtcgg tgaccgccag ttctacaatg
    27661 cgaccgctga cttccggcac cacatcaatg gtatctgctg acgcgtaagc gtcattagtt
    27721 gatggcgcac tgtctacgcg ccagataacg aaaacaaggg caaccagcgc caacgcaacc
    27781 actaacagag cagggaattt actgcgaggt gcttttttcg gcgtactttc catgagcgcg
    27841 tctcaaatag attagaaaaa tgccagccag aacagcatgg cgtagagagc aaaaagggcg
    27901 gtataaataa ttccgacaaa tgccagattg atatttgccc gctgaataac tcgcctcgtg
    27961 atgagcgtca aaataaggct ggcaatggcg cagaaaaacc agctgggata ataagcgccg
    28021 atcaccggga ttgccggaga taatgagcat cccgaaacca tgaagacggg aattatccag
    28081 gctgtttttt tgaggtgtat cgccatgcga gagagaactg ttggcatatt aaatacatcc
    28141 tgtctgctgc gaacaatatc tgtagtgaat atcaatatcc attgaaggcg tcagtatggc
    28201 tattttctcg atgaataaaa atagctttat tcagccaccg cattcataac ttatatattt
    28261 tatttattaa ttggctgtat atatttttaa aataaatgct tttgggggct tcagcgcccc
    28321 caaaagcgag tgacattaat ttggggttac gatattcacc cacaaatcga aattatccag
    28381 acaggcgata atttcttcca gtttcgcgcc attgccaata attttggctc tcttcgcttt
    28441 cac
//
LOCUS       DAJTYG010000047        27865 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTYG010000047 DAJTYG010000000
VERSION     DAJTYG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.047"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            241..1404
                     /gene="yqhD"
                     /locus_tag="OO568_002177"
     CDS             241..1404
                     /gene="yqhD"
                     /locus_tag="OO568_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6641519.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYKVAR"
     gene            1509..2336
                     /gene="dkgA"
                     /locus_tag="OO568_002178"
                     /pseudo
     CDS             1509..2336
                     /gene="dkgA"
                     /locus_tag="OO568_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
     gene            2536..3462
                     /locus_tag="OO568_002179"
     CDS             2536..3462
                     /locus_tag="OO568_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6641520.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKLIEAKTAARLKQ"
     gene            3513..3770
                     /gene="yqhH"
                     /locus_tag="OO568_002180"
     CDS             3513..3770
                     /gene="yqhH"
                     /locus_tag="OO568_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6641521.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(3813..6032)
                     /locus_tag="OO568_002181"
     CDS             complement(3813..6032)
                     /locus_tag="OO568_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6641522.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDCVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSLINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(6143..7555)
                     /gene="ftsP"
                     /locus_tag="OO568_002182"
     CDS             complement(6143..7555)
                     /gene="ftsP"
                     /locus_tag="OO568_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6641523.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(7630..8367)
                     /gene="plsC"
                     /locus_tag="OO568_002183"
                     /gene_synonym="parF"
     CDS             complement(7630..8367)
                     /gene="plsC"
                     /locus_tag="OO568_002183"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6641524.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(8601..10859)
                     /gene="parC"
                     /locus_tag="OO568_002184"
     CDS             complement(8601..10859)
                     /gene="parC"
                     /locus_tag="OO568_002184"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6641525.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNARAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(11405..11887)
                     /locus_tag="OO568_002185"
     CDS             complement(11405..11887)
                     /locus_tag="OO568_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6641526.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(11940..12332)
                     /gene="ygiW"
                     /locus_tag="OO568_002186"
     CDS             complement(11940..12332)
                     /gene="ygiW"
                     /locus_tag="OO568_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6641527.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESVKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            12484..13143
                     /gene="qseB"
                     /locus_tag="OO568_002187"
     CDS             12484..13143
                     /gene="qseB"
                     /locus_tag="OO568_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6641528.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLSSDFIRTVHGIGYTLGE
                     K"
     gene            13140..14489
                     /gene="qseC"
                     /locus_tag="OO568_002188"
     CDS             13140..14489
                     /gene="qseC"
                     /locus_tag="OO568_002188"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6641529.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTPDLNEINAADRMAQTPNKLKHDHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLIGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLLALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(14535..14867)
                     /locus_tag="OO568_002189"
     CDS             complement(14535..14867)
                     /locus_tag="OO568_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR6641530.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            15186..15767
                     /gene="mdaB"
                     /locus_tag="OO568_002190"
     CDS             15186..15767
                     /gene="mdaB"
                     /locus_tag="OO568_002190"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6641531.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTLK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            15798..16112
                     /locus_tag="OO568_002191"
     CDS             15798..16112
                     /locus_tag="OO568_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6641532.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSKAVKGDVLEMNIRILQP
                     GI"
     gene            complement(16160..18052)
                     /gene="parE"
                     /locus_tag="OO568_002192"
     CDS             complement(16160..18052)
                     /gene="parE"
                     /locus_tag="OO568_002192"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6641533.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGAVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(18081..18662)
                     /gene="yqiA"
                     /locus_tag="OO568_002193"
     CDS             complement(18081..18662)
                     /gene="yqiA"
                     /locus_tag="OO568_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6641534.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTRQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(18662..19489)
                     /gene="cpdA"
                     /locus_tag="OO568_002194"
     CDS             complement(18662..19489)
                     /gene="cpdA"
                     /locus_tag="OO568_002194"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6641535.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(19514..19936)
                     /locus_tag="OO568_002195"
     CDS             complement(19514..19936)
                     /locus_tag="OO568_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6641536.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTMVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(19937..20566)
                     /gene="nudF"
                     /locus_tag="OO568_002196"
     CDS             complement(19937..20566)
                     /gene="nudF"
                     /locus_tag="OO568_002196"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6641537.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            20771..22252
                     /gene="tolC"
                     /locus_tag="OO568_002197"
     CDS             20771..22252
                     /gene="tolC"
                     /locus_tag="OO568_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6641538.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            22400..23071
                     /locus_tag="OO568_002198"
     CDS             22400..23071
                     /locus_tag="OO568_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6641539.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            23077..24237
                     /locus_tag="OO568_002199"
     CDS             23077..24237
                     /locus_tag="OO568_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6641540.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQCGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(24275..25063)
                     /gene="ygiD"
                     /locus_tag="OO568_002200"
     CDS             complement(24275..25063)
                     /gene="ygiD"
                     /locus_tag="OO568_002200"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6641541.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLGIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            25206..25979
                     /gene="zupT"
                     /locus_tag="OO568_002201"
     CDS             25206..25979
                     /gene="zupT"
                     /locus_tag="OO568_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6641542.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(26037..26207)
                     /locus_tag="OO568_002202"
     CDS             complement(26037..26207)
                     /locus_tag="OO568_002202"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641543.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(26468..27121)
                     /gene="ribB"
                     /locus_tag="OO568_002203"
     CDS             complement(26468..27121)
                     /gene="ribB"
                     /locus_tag="OO568_002203"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6641544.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(27226..27374)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            27496..27786
                     /gene="ubiK"
                     /locus_tag="OO568_002204"
     CDS             27496..27786
                     /gene="ubiK"
                     /locus_tag="OO568_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6641545.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT         7022 a         7412 c         6912 g         6519 t
ORIGIN      
        1 ttattaactt tatccgccag caagcgacaa atctcttcac gtttcatatc gcgtaatttc
       61 ttaggaataa tgcggcaatt tgattgtgcg caattttgta gcatttctcc agcactctgg
      121 agaaataggc aagacattgg cagaaatgag cattgagagc cagggcgctg gcgatcacaa
      181 tgaaaaacat caggcagatc gttctctgcc ctcatattgg cccagcaaag ggagcaagta
      241 atgaacaact ttaatctgca caccccaacc cgcattctgt ttggtaaagg cgcaatcgct
      301 ggtttacgcg aacaaattcc tcacgatgct cgcgtattga ttacctacgg tggcggcagc
      361 gtgaaaaaaa ccggcgttct cgatcaagtt ctggatgccc tgaaaggcat ggacgtgctg
      421 gaatttggcg gtattgagcc aaacccggct tatgaaacgc tgatgaacgc cgtgaaactg
      481 gttcgcgaac agaaagtgac tttcctgctg gcggttggcg gcggttctgt actggacggc
      541 accaaattta tcgccgcagc ggctaactat ccggaaaata tcgatccgtg gcacattctg
      601 caaacgggcg gtaaagagat taaaagcgcc atcccgatgg gctgtgtgct gacgctgcca
      661 gcaaccggtt cagaatccaa cgcaggcgcg gtgatctccc gtaaaaccac aggcgacaag
      721 caggcgttcc attctgcaca tgttcagccg gtatttgccg tgctcgatcc ggtttatacc
      781 tacaccctgc cgccgcgtca ggtggctaac ggcgtagtgg acgcctttgt acacaccgtg
      841 gaacagtatg ttaccaaacc ggttgatgcc aaaattcagg accgtttcgc agaaggcatt
      901 ttgctgacgc taatcgaaga tggtccgaaa gccctgaaag agccagaaaa ctacgatgtg
      961 cgcgccaacg tcatgtgggc ggcgactcag gcgctgaacg gtttgattgg cgctggcgta
     1021 ccgcaggact gggcaacgca tatgctgggc cacgaactga ctgcgatgca cggtctggat
     1081 cacgcgcaaa cactggctat cgtcctgcct gcactgtgga atgaaaaacg cgagaccaag
     1141 cgcgctaagc tgctgcaata tgctgaacgc gtctggaaca tcactgaagg ttccgacgat
     1201 gagcgtattg acgccgcgat tgccgcaacc cgcaatttct ttgagcaatt aggcgtgccg
     1261 acccacctct ccgactacgg tctggacggc agctccatcc cggctttgct gaaaaaactg
     1321 gaagagcacg gcatgaccca actgggcgaa aatcatgaca ttacgttgga tgtcagccgc
     1381 cgtatataca aagtcgcccg ctaagctttt tacgcctcaa actttcgttt tcgggcattt
     1441 cgtccagact taagttcaca acacctcacc ggagcctgct ccggtgagtt catataaagg
     1501 aggaacgtat ggctaatcca accgttatta agctacagga tggcaatgtc atgccccagc
     1561 tgggactggg cgtctggcaa gcaagtaatg aggaagtaat caccgccatt caaaaagcgt
     1621 tagaagtggg ttatcgctcg attgataccg ccgcggccta caagaacgaa gaaggtgtcg
     1681 gcaaagccct gaaaaatgcc tcagtcaaca gagaagaact gtccatcacc actaagctgt
     1741 ggaacgacga ccacaagtgc ccccgcgaag ccctgctcga cagcctgaaa aaactccagc
     1801 ttgattatat cgacctctac ttaatgcact ggcccgttcc cgctatcgac cattatgtcg
     1861 aagcatggaa aggcatgatc gaattgcaaa aagagggatt aatcaaaagc atcggcgtgt
     1921 gcaacttcca gatccatcac ctgcaacgcc tgattgatga aactggcgtg acgcctgtga
     1981 taaaccagat cgaacttcat ccgctgatgc aacaacgcca gctacacgcc tggaacgcga
     2041 cacacaaaat ccagaccgaa tcctagagcc cattagcgca aggaggaaaa ggcgttttcg
     2101 atcagaaagt cattcgcgat ctggcagata aatacggcaa aaccccggcg cagattgtta
     2161 tccgctggca tctggatagc ggcctggtgg tgatcccgaa atcggtcaca ccttcacgta
     2221 ttgccgaaaa ctttgatgtc tgggatttcc gtctcgacaa agacgaactc ggcaaaattg
     2281 caaaactcga tcagggcaag cgtctcggtc ccgatcctga ccagttcggc ggctaacgtg
     2341 caaattctcc cggtggcggt aatgttccgc taccggactt ttcagaaatc atttattccc
     2401 ctcgcgtccc gcccgttgtt actcttcctt gttcaggaat gccaaatata aggacatcat
     2461 catgcagagc cggaagctct taaaagaaca actcatctat atccgggata aacgcaacgg
     2521 agaggtgaaa aacagatgaa aataatactt ctgtttttag cagccctggc aagttttacc
     2581 gtacacgcgc agcctccttc acagaccgta gaacaaacag tccggcagat ttatcagaac
     2641 tataaatcag atgccagtac gccttatttc ggtgaaaccg gagagcgggc gataacttca
     2701 gcgcgtattc aacaggcgct taccctgaac gacaatctta cactaccggg caatattggc
     2761 tggctggatt atgatccggt ttgtgattgt caggattttg gcgatctggt gttagaaagc
     2821 gtagcgataa cccaaactga cgtcgatcat gccgatgccg ttgtgcgctt tcgtatcttt
     2881 aaagatgata aagaaaagac cacacagaca ctgaaaatgg tggcggaaaa tggtcgttgg
     2941 gtcattgatg atattgtcag caatcatggc agcgtcttac aagcagttaa tagcgagaat
     3001 gaaaaaacgc tggccgcttt agcttcgttg cagaaagaac agccagaatc ctttgttgct
     3061 gaactctttg aacatattgc tgattatagc tggccgtgga cgtgggtggt ttccgactct
     3121 taccgccagg cggttaatgc cttctataaa accaccttca agacggccaa taatcccgat
     3181 gaagatatgc aaatagaacg gcaatttatt tacgacaatc cgatctgttt tggcgaagag
     3241 tcgctatttt cacgcgttga tgaaattcga gtcctggaga aaaccgccga ttccgcccgc
     3301 attcatgttc gttttacgct gaccaatggc aacaatgaag agcaagagct ggttttacag
     3361 cggcgcgaag gtaagtggga aatcgctgat tttatccgcc cgaacagcgg cagcctactt
     3421 aagctgattg aggcaaaaac cgccgccaga ttaaagcaat gagctgaatt aaataacaat
     3481 tagccggaac aataaataaa agggaacact atatgaaaac gattttcacc gtgggagctg
     3541 ttgttctggc aacctgcttg ctcagtggct gcgtcaatga gcaaaaggtc aatcaactgg
     3601 cgagcaatgt acaaacatta aatgccaaaa tcgcccggct tgagcaggat atgaaagcac
     3661 tacgcccaca aatctatgct gccaaatccg aagctaacag agccaatacg cgtcttgatg
     3721 cacaggacta ttttgattgc ctgcgctgct tgcgtatgta cgcagaatga taaaaaaatc
     3781 cccggcagca tgtcagttgc cggggatttt ttttaacgtc caaccgccgc tttagggcgt
     3841 ttcttcgcac cagcattcac cggacgagat tgcgtagacg acgctttttt tgccgtagca
     3901 ggcgtctgac gctgagtcgc catcggcgta tgtttagtca acgccggacg ggtattgcgg
     3961 ttctggcgac gagcttcacg catctcttca atggttggcg caggcactaa gcaatcgcga
     4021 cggctgccaa tcagatgctt tttgcccatc gcttccagcg cctggcggat taacggccag
     4081 tttgccggat cgtggtaacg caacaacgct ttatgcaaac gacgctgttt gtcgcccttc
     4141 ggtacgaaga cgtcttcgct cttataacca atcttcgcca gcggtttttt cccggtgtaa
     4201 tacatggtgg ttgagttcgc cagcggcgat gggtagaagt tctgtacctg gtcgagacgg
     4261 aaacgatgct ttttcagcca cagcgccaaa ttcaccatat cttcatcacg cgtaccgggg
     4321 tgcgcagaga tgaaatacgg gatcagatac tgctctttac ctgcctgttt cgagtaggta
     4381 tcgaacagct ctttaaagcg gtcatagctg cccatgcccg gcttcatcat cttcgacaac
     4441 ggcccttctt cggtatgttc cggagcaatc ttcagataac cgccgacgtg atgggtcgcc
     4501 aactctttga tatagcgcgg atcttccacg gcgatgtcat aacgtacgcc tgaggcgata
     4561 aggatttttt taatgccttt cagatcacgc gcacggcgat agaggttgat cgtcggttcg
     4621 tggttagtgt ccatgtgcgg acaaatatcc ggataaacgc acgacaaacg gcgacaagtt
     4681 tgttcagcgc gtggcgattt gcagcgcaac atatacatgt tggcagttgg cccaccgaga
     4741 tcggaaatca cgcccgtaaa acctggaacg gtgtcgcgga tcgcttcgat ctcattaatg
     4801 agcgaatctt ccgaacggct ctgaataatg cgcccttcgt gctcggtaat agaacagaaa
     4861 gaacagccgc caaagcagcc gcgcataatg ttgaccgaaa aacggatcat ttcgtaagcc
     4921 ggaatacggg cattgccata ggccggatgt ggcacgcgct tgtacggcag cgcaaaaacg
     4981 cagtccatct cttcggtaga aagcgggata gcaggcgggt tgatccacac atagcggtca
     5041 ccgtgttttt gcatcaatgc gcgggcgcag cctgggttgg tttcgtggtg cagaatgcgc
     5101 gaagcatggg cgtacagcac tttatcgccc ttcactttct cgaaagaagg cagcaatacg
     5161 taggtttttt cccacggttt ctggcgcggt ggctgcacga ttacagcttt cgcttcctgc
     5221 tttttcggcg caacaggttt gttatccgca cacggcaaat cttcgccata cggatgcggg
     5281 attgggtcga ttttccctgg ggtatcaaga cgggtggaat ccacaccgct ccagccaggc
     5341 agcgcctctt ttacgataat tgcggtatta cgcacatcgc ggatttcact aatcggctcg
     5401 cccatcgcca gacgatgcgc tacttccacc agcggacgtt caccgttacc aaacatcagc
     5461 atgtcggctt tcgaatctac cagaacggaa cggcgcacgg tatcggacca gtaatcataa
     5521 tgcgcggtac ggcgcaggct agcctcaata ccgccgagga tcaccggcac atctttccac
     5581 gcctctttac aacgctgggt ataaaccagc gtggcgcgat ccggtcgctt acccgcgaca
     5641 ttatctggcg tgtaggcatc atcatgacgt aaacggcgat cggcggtata gcggttgatc
     5701 atcgaatcca tgttgccagc agtaacacca aaaaacagat tcggtttacc cagacgcata
     5761 aagtcgtctt tgctgctcca gtccggctgg gcgatgatcc cgacgcgaaa gccctgcgct
     5821 tccagcatac gaccgcaaat cgccatcccg aagcttgggt gatcgacata cgcgtcgcca
     5881 gtaaccaaaa tgatgtcgca gctatcccag ccaagttgat ccatctcttc acgagacatc
     5941 ggcaaaaacg gtgccggtcc aaaacaggcg gcccagtact gcggccagga gaacaggtcg
     6001 cgatccggtt ggatcaggga gatagagctc attttgcttc cagaaatgat aaaaaaataa
     6061 tcaaaggccg gggattataa gccggaacga aagagaaatc gaaaggtatt ccatactcgc
     6121 cctcctcggg cgagtatgaa gattacggta ccggattgac caacagttgc ccaatcgagc
     6181 cacggtccgc catttccagc gtctgactgt tgaagtagaa cgggaagtgc gcccaggaag
     6241 gctgaccgaa ataaacaagc agctccactt gtccgtcaac ccaaacggta tctttccagc
     6301 ctctgtcttc cgggaacggc atcgcgccat tcacgttacg gatctggaac attacgcctt
     6361 caatatggaa cgcttgcggc tcgtccgcgc gtaccgtcca gcgttcccac gttccttgct
     6421 gcgcggtgac atcaatacgg ttgacgtccc acagctgtcc attaataccc gggtcatcac
     6481 ccagactgat atcgcgactg cgaattggcg aaccagccat gatttcagtt ggcagcaagc
     6541 gcatcggaag actgtctgtg accagcggca gaaggccggt tgggcgcagc gttagcacca
     6601 aggtagaaac cagaatactg gatggctcaa agaagccgcg aatacgatca acaatgctcg
     6661 ccgcttcgcc acaggtgatc gacacttcat cgccgttgct catatccacc agaatctcgc
     6721 ggcgctcgcc cggtgccagc gaaagttgct tcaccgatac aggagcaggc aggaatccct
     6781 gatcgccaga aatcacatgt aacgggcgac catcgctcat ctgtagttga tagcgacgag
     6841 agttcgacgc gttcagcagt cgcaagcgca cccagccacg cgagacttca acgtacgggc
     6901 tttgtacacc gttaaccagc agcgtatcac caacaaagcc gccgcttccc ggttcgttgt
     6961 attctggcgt accaaagtta tccagccgtt tatcctggat aatgaccgga aaatcatcca
     7021 caccataatg gttggggata ggcagcgact tgctgacttc atcttccacc agccacattc
     7081 cggcaaggcc gttatagacc tgctgagccg tgcggttggg agtattggcg tgataccaca
     7141 gagtagctgc gttctggcga ataggcagta cgggtgccca gtcagcgttt ggcgacatca
     7201 tccgtgccgg accgcccatc agcgggcctg gtacctgtag cccggccacc gtcattgaga
     7261 cattttctgt caggcggttg ctgtaaataa gcttaacatc gtcgcccttc cagacgcgga
     7321 tagtcggccc caggtaacga ccattgattc cccagaccga cgcgcgtgtc cctggcgtaa
     7381 atgaccagtg cgcacgttgt acagtcataa acagcggttg cccacggcga gattcaagta
     7441 gcggcggaac gggtagcggt tgctgttgcc cggctgcgct ggccttcagg ggaacagcgc
     7501 ctgcacaaag tgcaatcccc gatgcctgaa tgaactgacg ccgactgagt gacatatttg
     7561 ctccatgtaa aactggctaa agtaataccg tgcaggaaag tgttccccgc aaaaatacag
     7621 attgccgact taaacttttc cggcggcttc gcgttctgcg acttctttat cgagctcggc
     7681 gattttttgt tccattatcg aacgacaatg ggcagccagc tcacgaacct gatctttgcc
     7741 atactgactg acgtcaattg gcggcagcat ttcgacaatc accagaccgt tgtgcagtcg
     7801 attaagatta atcttattcg aagttgtaga gacgcacacg ggaataatcg ggacgcccgc
     7861 cgcaattgcc gcgtgaaatg ctccagtctt gaacggtagc aggccgcgac cacggctgcg
     7921 ggttccttcc gggaacatcc agatggaaat acggcgtttt ttgaaatgat tcacgacttc
     7981 cgcaatggtg ccgtgagctt tagtgcgatt gtttctgtcg atcaataagt tgccggttaa
     8041 ccagtacaac tgcccgaaga aggggatcca cagcaagctc tttttaccta ccgtcaccgt
     8101 cggcggttgc acaatgttcg atgctgtcac catgtcatag ttgttctggt ggttagcgat
     8161 atagatagca ttgccgtagc tttcagcatc tgcaggtttg cggcactcaa ctttcaggcc
     8221 gaacagtggc gcaagacggc caaacatatg cccaaaggtg gccacatgtt tcgggttacg
     8281 cgggctgaaa aggcagtaaa tggagccgaa tacacagact aagatgctgt aaatcacggt
     8341 aataataaga cgaaagatat atagcataac accctctgaa ggtctgagag actggcattg
     8401 tacgtcacct gaaatcaggt tagggcattg ttaaaagccg ctctctggaa aagcgtattg
     8461 ttaatcgcaa cgcatgaata aacaacggtt tcaagggaat ttttatcgtt tccgggggtt
     8521 gaatacgctg ccggatgacg acttaacgtt tcatccggcg ttccttgcta gcgggaggaa
     8581 acagcgccct ccccggcata ttactcttcg ctatcaccgc tgctggcacg gcgaggagag
     8641 tcgatctcaa cacgatcgat acgctgcaaa ccgcgcatca acgtaccgcg gcgtccacgt
     8701 tcgccagtga ctttctgtag ctcttccgga cgcagtttaa ttttgcgttt cccaacatga
     8761 atggtcagcg tgctttgcgg cggcagaacg tacagttgcg ccagaccatc ctcgccacgt
     8821 gcggcttctg ccgatggaat gttgataatc ttgttgcctt tgcccttcga cagctgcggc
     8881 agatcgctta ccgggaacat caacatacgg cctgcctgag tgattgccag cagcatatcg
     8941 gaagcatctt caatcaccac cggcggcata acatgggcat tttccggtaa ggtgatcaaa
     9001 gccttacccg cacggttacg cgccaccaga tcgttaaagg tgcagacgaa accgtaaccc
     9061 gcatcggaag ccatcagcag tttctgatcg tcgctttcca tcagcatatg gtcaacggtc
     9121 gccccaggcg gcaacgttaa tttgccggtg agtggttcgc cctgaccacg cgccgacggc
     9181 agcgtaatcg ggtcgatggc atagctacga ccggtggaat caacaaacac taccggttgg
     9241 ttactcttac ctttcaccgc cgctttgaag ctatcacccg ctttataatt caggcccggc
     9301 gcgtcgatat catggccttt agcgctgcgt acccagccca tctgcgacag cacaatggtg
     9361 acaggttcag acggcagcat gtcgtgctcg ctcatagctt tcgcttcttc gcgttcctgc
     9421 aacggcgaac gacgatcgtc accgtaggct tgcgcgtctg cctgcagttc tttcttcagc
     9481 aggttattca ttttacgctc ggaagccaaa atgccctgca actggtcgcg ctctttttcc
     9541 agctcactct gctcaccgcg aatcttcatc tcttccagtt tggcaagatg acgcagtttc
     9601 agttcgagga tcgcttccgc ctgggtttcc gtaaggccaa accgcgacat cagcgccggt
     9661 ttcggttcat cttcgttacg aatgatctca atcacttcgt cgatgttgag aaacgccacc
     9721 agcaaacctt ccaggatatg caggcgcttg aggactttct ccagacgata gttcagtcgg
     9781 cggcgcacgg tatcgcgacg gaacaccagc cattcggaga ggatttccag caggtttttc
     9841 accgccggac gaccatccag accgatcata ttgaggttaa tgcgatagct cttttccaga
     9901 tcggtggtag cgaagaggtg gttcatcacc tgatccatat ccacgcggtt ggaacgcggc
     9961 acaatcacca gacgggtcgg gttctcgtgg tcagattcat cgcgcagatc gtcaaccatc
    10021 ggcagctttt tgttgcgcat ttgcgcagca atttgctcca gtacgcgcgc acctgaaacc
    10081 tgatgcggta atgcgctgat aaccaccgcg ccatcttctt tcttccacac cgcgcgcata
    10141 cgcactgaac cacgtccgtt ctcgtagatt ttacgaatct cggcgcgcga agtgataatt
    10201 tccgcttcag tcggataatc cggcccctgc acgatatcca gcagctgatc gagcgtggtt
    10261 ttcggctggt cgattaatgc gattgccgcc tgagctactt cacgcaggtt atgcggtgga
    10321 atatcggtcg ccatgccgac ggcaataccg gtggtgccgt taagcaaaat gtttggcaga
    10381 cgggcaggta gcattttcgg ctcctgcaaa gtgccgtcga agtttggcac ccagtcagcc
    10441 gtcccctgcc ccagctcgct caatagcagc tcggaatatt tcgacaaccg ggattcggtg
    10501 taacgcattg ccgcgaacga tttcggatcg tccggcgcgc cccagttccc ctgaccatca
    10561 accagcggat aacggtaaga gaacggctgc gccatcagga ccatcgcttc ataacaggcg
    10621 ctatcgccgt gcggatggta tttacccagt acgtcaccga cagtacgggc cgatttttta
    10681 aatttggctc tggcattcag gcccagttca gacatcgcat acacaatgcg gcgctgaaca
    10741 ggtttcagac catcaccaat aaacggcaac gcacggtcca tgatcacgta catggagtag
    10801 tttaagtagg cgttttccgt aaattcatgt agcgcaaggc gctctgccat atcgctcatt
    10861 aattctgatt cctcaactta ttcgccagcc ttcaaagggg caatattgcc ggagatacta
    10921 cattatctgc cgcgtcgagt cacaggagat cagcgtgaat agcgccgccg gacgtgctcc
    10981 gacggcgata aaggcatcaa tgtgccttga tatacaactc tttgctgtag acttgatcgg
    11041 catagagggc agcacaaggt tgcgcccatg ccacaccact cggcatgggg gtccgctccg
    11101 ggcgattcga gaagttgagg ttatgcgagt ccagggaagc ctaaagcatc aaagtagtcg
    11161 actgtaagta tacggttcag cccagtttag ctgtgtctcc accagtgccc cgcgcctcct
    11221 gccataagtg cggcttgctc atggataccc ccgagacggc ttacggcgca ccggataata
    11281 aatttcgcgt atacatggta ttccttaccg gagaaattat aagcccaaca acaatacgcc
    11341 tgtgacattg ttcgctcaaa cttatcgcga gtgatttggc tcacactccg gtaaagattc
    11401 cccatcacgc caacggcaca taaacatccg tttccagtcg ctgctctgtc accccttcgg
    11461 caagattggt gtagtgaaac agaatcggtg ctttacgcat tttctcgccg cttgcaggca
    11521 gccagtggcg aataatgccc catatcgtgt gtgaaatatc gtccagctcg ccaacgtggc
    11581 gggccacggc ataacgtcca ccggtaagtt caccattgct cacaccataa cgattatcgg
    11641 gaatcggttc gctaacgctg ccgcagatat caaagcgaaa cgcttccggt gcggtggtgg
    11701 cagggtcgtc ccaggcgacg ccaaaagtct ggctttggtt aacaggagaa agccccgtct
    11761 ctttacgcca catgataaat ttcgccacac tgtagttgag caactcaggg ctacagcggt
    11821 gcggcaacat cgccacaggg attgatggga aatcgataat gtttacatcc agtgtcaggt
    11881 ttgtcatttt gcctccctgc gaaattattc ccgggaacgc gtcccgggag cggtaacaat
    11941 tacggattta ctttgcggat ctgtttgacg tcaatttcaa cagaattcca gtctttatcg
    12001 acttcaccct gaatctcaac cgtatctttc ggcgtcaccg tcacaccgtt ccagcgtttg
    12061 tgatcgatat caacattgat agtaccgctg gcatctttaa acacgtagag atcgtcagag
    12121 atgcgttcaa cgatattgcc gcgcagggtt acccaggtgt cgtcacgcag ggattttacg
    12181 ctttctacag tcgttacgct gccgttcggc ccctgaaatc ctccggcctg actttgcgtt
    12241 gccgatgggc cagaaaaacc gccctgctct gctgccatca ccggtgcact gcacagggcc
    12301 attactgcga ttactgctgc gaattttttc atgtttatta ctccctttaa tgtctgtttc
    12361 cgagcattta acaagatagt ccttaacaac ttcttaaggg aaaaaaataa aatttaatgc
    12421 tgtacagagc gcgttacaac acggtttact ggcagcaaat acggttatcg cagggatgaa
    12481 aaaatgcgaa ttttactgat agaagatgac atgctgattg gtgacggcat caaaactggc
    12541 cttagtaaaa tgggttttag tgtcgactgg tttacacaag gtcgtcaggg aaaagaggcg
    12601 ctttatagcg caccttatga tgcggtgatc ctggatttaa ccttaccagg catggatggt
    12661 cgcgatattt tgcgcgaatg gcgagagaaa ggtcagcgtg agccggtact gaacctgacc
    12721 gcgcgtgatg cgttagcgga acgtgtagaa gggctgcgtc tgggagctga cgattatctg
    12781 tgtaaacctt ttgcgttgat agaagtcgcc gccaggctgg aggctctgat gcgccgaacc
    12841 aacggccagg ccagcaacga gctgcgccac ggtaacgtca tgctcgaccc cggcaaacgt
    12901 atcgccacgc tggctggcga acccttaaca ctgaaaccaa aagaatttgc cctgctggaa
    12961 ttactgatgc gtaacgctgg tcgggtactg ccgcgcaaac tgattgaaga gaaactgtat
    13021 acctgggacg aagaggtcac cagtaatgcc gttgaagtgc atgtgcatca tctgcgacgc
    13081 aaactcagta gtgattttat tcgtaccgtg catggtattg gctacacatt aggtgagaaa
    13141 tgaaatttac ccaacgtctt agtctgcgcg tcaggctgac gctaatcttt ttaattctgg
    13201 cctcggtgac ctggctgctt tccagctttg tcgcctggaa acaaacaacg gataacgtcg
    13261 atgaattgtt cgacacccaa ctgatgctgt ttgccaaacg gttaagtacg cccgatctca
    13321 acgaaatcaa cgcggcggat cgcatggcac agacgccaaa taaattaaaa cacgatcatg
    13381 ttgatgacga tgcgctgacc tttgccatct ttacccacga cggcagaatg gtccttaatg
    13441 atggcgataa cggagaagat attccctata gctatcaacg ggaaggtttt gctgacgggc
    13501 aactgatcgg tgaagacgat ccttggcgtt ttgtctggat gacctcacct gatggcaaat
    13561 atcgcatcgt tgttggccag gaatgggaat accgtgaaga catggcgctg gcgattgttg
    13621 ccgggcaatt gatcccgtgg ctgctcgcac tgccgattat gttaatcatc atgatggtac
    13681 tactgggtcg tgaactcgcg ccgctgaaca aactggcgct ggcactacgt atgcgtgacc
    13741 ctgactcgga aaaaccacta aacgcgactg gcgtacccag cgaagtgcgg ccactggttg
    13801 agtcgctaaa tcaactgttc gcccgcacac atgcgatgat ggttcgagaa cgacgcttta
    13861 cctccgatgc agctcacgaa ctacgtagcc cgttaacagc gctgaaagtg caaaccgaag
    13921 ttgcgcagct ctctgacgat gatccgcagg cgcggaaaaa agcactgctc caattacatt
    13981 ccgggatcga tcgcgctact cgtctggttg atcaactgct cacgctatcg cggctggact
    14041 cactggataa ccttcaggac gtcgcggaga tcccgcttga agatctccta caatcgtcgg
    14101 tgatggatat ttaccacacg gcgcagcagg cgaaaattga cgtgcgactg acactcaatg
    14161 cccacggcat caaacgcacc ggacaaccgc tattgctaag tttgttggtg cgaaatttgc
    14221 tggataacgc cgtgcgctac agtccacagg gcagcgtgat agacgtcacg ctgaatgctg
    14281 ataatttcat cgtgagggat aacggccccg gtgtgacacc agaggcactg gcgcgaattg
    14341 gcgaacgctt ctatcgccca cccggacaaa ccgctaccgg cagtggactt gggctatcga
    14401 ttgtccagcg aatcgccaag ctgcatgaca tgaatgttga gtttgggaat gcggaacaag
    14461 gtggatttga ggcgaaggta agctggtaac ttctctgtct atgagagccg ttaaaacgac
    14521 tctcatagat tttactaata gcaaaatata aaccgtcccc aaaaaagcca ccaaccacaa
    14581 accagacaaa taacgagcga gaatacatat agccaaaact cgaacatttc ccgcataaag
    14641 agttttctta agataagaat aataagtggc gtaagaagaa aaaatgctgc aatcattgct
    14701 tgcaatatac gatcttcgcc tgaaacggaa ggtatactgc aaatattgcc tcccgactca
    14761 agcatccagt catacttatt gaaagcaaaa aaagaatcta ttaaccaata agtaagcatt
    14821 atatatagca atgcttcaaa tattgtttta attttctgct ttaacatata tcagtacgct
    14881 cataaaaatc catttataat tatcaacatg acacgttgat attttcatct tttacttatt
    14941 caaagccata ttttcaatcc ccaaaaaaac cgaaccaaac gtgtaaatta ttccggtacg
    15001 agtaagaatt atgaggaatg gctatcagta ttgtcatttt cagaaaatat ttatcctgca
    15061 tcggtgagtc agagctagat cagacttttg ctaaattcgc aaaagacttt gcacattttg
    15121 ctaatttcac cgtaccgctc tgtgacgtac tatagtcgac aaacgtctca ccttgaggtt
    15181 aaaaaatgag caacatcctg attatcaacg gcgcgaaaaa attcgcccac tccaatggtc
    15241 aactgaacga caccctgacc gaagtcgcgg atggcacact gcgcgacctc gggcatgatg
    15301 tccgcatcgt tcgcgccgac agcgactaca atgtcaaagc ggaagtacaa aactttctct
    15361 gggctgatgt ggtgatctgg cagatgccag gctggtggat gggcgcgccg tggacagtga
    15421 aaaaatatat tgatgatgta ttcaccgaag gtcacgggac tctgtatgcc agcgatggtc
    15481 gtaccctcaa agatccgtcg aaaaaatacg gttccggcgg cctggtacag ggcaaaaaat
    15541 atatgctttc tctaacctgg aacgcaccaa tggaagcctt caccgaaaaa gatcagttct
    15601 tccacggcgt tggcgttgac ggtgtgtatc tgccgttcca taaagcaaac caattcctcg
    15661 gtatggaacc gctgccgaca tttatcgcta atgacgtgat aaaaatgcct gatgttcccc
    15721 gctatactga agaatatcgc aagcatcttg tggaaatttt tggttaacta gagctcaggc
    15781 tttagaagga gttaaccatg cttaccgtaa tcgcagaaat ccgtactcgt cctggtcaac
    15841 atcaccgtca ggcggtattg gatcagtttg ctaaaatcgt tccaaccgta ctgaaagaag
    15901 aaggttgcca cggctatgcg ccaatggtgg attgcgctgc tggcgtgagt ttccagtcta
    15961 tggcaccgga ttctatcgtg atgattgagc agtgggaaag catcgcgcac cttgaagcgc
    16021 atctgcaaac cccgcacatg aaggcgtaca gcaaagccgt aaaaggcgac gtgctggaga
    16081 tgaatatccg tattctgcag ccagggattt aatcctgcct tgtttgcccg gccatcctga
    16141 ccgggcaatg ttctttcctt taaacctcaa tctccgccat gtcgcctttc tcttgcaacc
    16201 agttgcggcg atcttccgag cgtttcttcg ccagcagcat atccatcatc gcgtcagtac
    16261 gctgatcgtc ttcatcatcg atagtcaact gcaccagacg gcgagtgttc ggatcaagcg
    16321 tggtttcacg caattgcatc gggttcattt cccccagacc tttaaaacgc tggacgttcg
    16381 gcttgccttt cttgcgtttt aattgctcaa gtacgccctc tttctcttct tccgtcagcg
    16441 cgtaataaac ctctttcccg agatcaatac ggtagagcgg tggcagtgcg acgtaaacgt
    16501 gaccgtgttt caccaacgcg cggaagtgtt ttacgaacaa agcgcagagc agcgtggcaa
    16561 tgtgcagacc atcagagtcc gcatccgcga ggatacagat tttgccataa cgaagctggc
    16621 tcagatcgtc gctgtcagga tcgataccga tcgctaccga aatatcatgc acttcctgcg
    16681 aggccagcac ttcgtcggaa gaaacttccc aggtgttaag gatcttacct ttcagcggca
    16741 tgatcgcctg atattcacga tcgcgcgcct gcttggcaga tccgcctgcg gagtcacctt
    16801 ccacgaggaa cagttcggta cggttaaggt cctgcgcggt acaatcagcc agtttgccag
    16861 gcagcgccgg gccgctggtc agctttttac gcaccacttt tttagccgca cgcatacgac
    16921 gctgggcgct ggaaatcgcc atctctgcca gcaactccgc cgcctgaacg ttctggttca
    16981 gccacaggat gaaggcatct ttcaccgcgc ccgaaacgaa tgccgcacac tgacgcgaag
    17041 agagacgctc tttggtctgc ccggcaaact gcggatcctg cattttaacc gacagaacat
    17101 aggcgcagcg atcccagata tcttccgccg acagcttcac gccgcgcggc agaatgttgc
    17161 ggtattcaca gaactcacgc atcgcgtcca acaggccctg acgcagacca ttaacatggg
    17221 taccgccctg catcgttggg ataaggttga cgtagctttc ggtcagcagt tcaccgcctt
    17281 ccggcagcca cagtagcgcc cagtccacag cttcagtatc gccagcgaaa ttaccgataa
    17341 acggtttttc tggcagcgtc ggtaaaccgt ttaccgcttc cgccaggtaa tcattcagac
    17401 cgtcctgata gcaccagcgc tgttcggtgt tgttgatctc atctttaaag gtaatctcaa
    17461 cgccggggca cagtactgct ttggccttca gcacatgcgt caggcgtgaa acagaaaaac
    17521 gcgggctgtc aaagaaggtt tcatccggcc agaagtgcac gctggtaccg gtattgcgtt
    17581 taccgcaagt gccgacaacc tgtaaatcct gcaccttttc gccattttca aaggcgatgt
    17641 tataaacctg accatcgcgg cgcacgttaa cttctacgcg cttcgacaag gcgttaacta
    17701 ccgaaatccc cacgccatgc aggccgccag agaactggta atttttgtta gagaatttac
    17761 cgcctgcatg cagacggcaa agaatcagtt caaccgccgg tactccttct tccgggtgaa
    17821 tatccaccgg catcccgcgc ccatcgtcaa tcacttctaa cgactggtca gcatgtaaga
    17881 ttacatccac gcgttttgcg tgacccgcca gtgcttcatc gacactgtta tcaataactt
    17941 cttgccccaa atggttaggg cgagtggtat cggtatacat ccccggacgg cggcgaaccg
    18001 gctcaagccc ggtgagtacc tcaatggcat cagcgttata agtttgcgtc atggtttaag
    18061 ttagcaattc gagttgatcg tcagagatgg tgcagaccaa gaaaatcgac gatcgggttg
    18121 aaataatctt cgaagcccgt gaatgcgtgg ttgccgcctt ctatgacagt ctggcggcag
    18181 gaagcgtagt acgccaccgc ctggcggtaa tccagcactt catctcccgt ctgttgcagc
    18241 agccagatca aatccggcgc ttccagcggg tcaatctgca tgactttgag atcgtaaata
    18301 tggcgtgact ctagcacata ttgctgcctg gtgtaggggt tctcgttctg accgagatag
    18361 tccgtcagca attcaaacgg gcgtaccgcc gggtttacca ccactgcggg cagcataaaa
    18421 cattgcgaca accaggtggc gtaatatccc cccagtgacg aaccgacaat acccagcgaa
    18481 tcgccgccat gttccaggac aatggattcc agcagttctg ccgcgtcgga aggatacggc
    18541 ggcaactgcg gaatgatcat ctcaacgtca gggtgatgtt ccgccagcca gttttttaac
    18601 aagctcgctt ttgcagagcg cggcgagctg ttgaaaccgt gtaaataaag aagcgtagac
    18661 atcagtagcc ttctgaagcg gtatcaggtt ggaaacgtgt gtccgccagg cgatgcacct
    18721 cggtggtcag cgtgccatca gcatgtaact cgagagtacg ccagccgggc gcgatagtat
    18781 ccagcgtaaa gttggaacag tgcggcttaa actgcacaca ggtcgacggc gttgccagca
    18841 ggcggcgacc gttccagtcg agatccagct cctgatgaat atgaccgcac agcaagtatt
    18901 tgacgtgcgg aaacttcgcc agcacggtat ccagttcgcc cgcgttacgc agactgtgtt
    18961 gatcgagcca actacaaccc gcaggtagcg gatgatgatg cagcagcagc aacgtatggc
    19021 gttctggcgc atcggccagt ttacgttcca gccactcaag ctgaaactcg ctcagctcac
    19081 cgtgcggcac gccaaacacc tggctatcca gcaacaggat ttgccattgc tcaccaataa
    19141 acacccgctt cgccggggag atacccgcat cctgtaacgc gctgtacatc gcgggctgga
    19201 aatcgtggtt gcccggcagc cagacgcagg gcgcacgaaa acttgcgatg ccttcagcga
    19261 aatgctgata ggccgcagag gattgatcct gcgctaaatc acctgtcgcg acaatcaggt
    19321 cgaattcgtg ctggtgtgga cgaatcgcct ccagcaccgc ctggtagctt tcccaggtgt
    19381 ttacccctaa cagggcttcg tgcttttgtg caaacaggtg agtatcggta atttgtaaaa
    19441 tccttcctct ggcctcacca gccagaggaa gggttaacag gctttccaaa tggtgtcctt
    19501 aggtttcacg acgctagtaa accggaatcg ccatcgctcc atgtgctaaa cagtatcgca
    19561 accagtccgc taaaaactgg ttaatttgat gcttttcgtc gcgttgatgc aactttttat
    19621 taggataatc ataccgcgct ttgaagcgaa aaatctgctg gcttgaacac acttcagcca
    19681 ccatcgcgtc atgatacaga cgcaccgtca ttgacggaag gctccagtaa ctgatcgcgg
    19741 gcgcagtctg ttctattgtc accatggtag tgtatcgggt cgattccaca atcgtcagcc
    19801 gatattgtgc gtttgccacc tgatagctta cagtttcgcc gggtgcgtca ttgcgcggta
    19861 acaaacggcg caattgtgaa aagttcatct cgcacaggcg catcatttca ggaaagtcag
    19921 gtgtgtaacg cttcatttat gcccactcat tttttaacgc ttgatgatgc agctgcagcc
    19981 attgcaaagc gatgaccgac gctgcgttgt cgattttccc ctcttctacc cactggtatg
    20041 cctgttcccg gcttaccaca tgaacgcgaa tatcttcgtt ttcatcagcc agaccatgaa
    20101 taccgcttgc ggtcgtggcg tccacttcgc ccaccataat tgacgaacgc tcactggtgc
    20161 cccccgggct tgccaggaaa cttaacaccg gtttggtccg tttgactatc agtcccgcct
    20221 cttcaatcgc ttcgcgacgg gcaacatctt ccacactttc accctcttca atcatcccgg
    20281 caaccatctc cagtagccag ggggtttcgc tggtgtcgta cgcggcaatc cgaatctgct
    20341 caatcagcac aacttcatca cgcactgggt caaagggtag caagactgcg gcgtgaccgc
    20401 gctcaaaaat ttcccgccgt acctcatgac tcatttgccc gttgaataga cgatgacgaa
    20461 atctataaag atctaatgaa aaaaagccgc gataaagtgt ttctcgtgca ataatttcta
    20521 catcgttttt gccaaatgta acgggcaggt tgtctggctt aagcattgtt aatgtcctgg
    20581 cactaatagt gaattaaatg tgaatttcag cgacgtttga ctgccgtttg agcagtcata
    20641 tgttaaattg aggcacatta acgccctatg gcacgtaacg ccaacctttt gcggtagcgg
    20701 cttctgctag aatccgcaat aattttacag tttgatcgcg ctaaatactg cttcaccaca
    20761 aggaatgcaa atgaagaaat tgctccccat tcttatcggc ctgagccttt ctgggttcag
    20821 ttcgttgagc caggccgaga acctgatgca agtttatcag caagcacgcc ttagtaaccc
    20881 ggaattgcgt aagtctgccg ccgatcgtga tgctgccttt gaaaaaatta atgaagcgcg
    20941 cagtccatta ctgccacagc taggtttagg tgcagattac acctatagca acggctaccg
    21001 cgacgcgaac ggcatcaact cgaacgcgac cagtgcgtcc ctgcagttaa ctcaatccat
    21061 ttttgatatg tcgaaatggc gtgcgttaac gctgcaggaa aaagcagcag ggattcagga
    21121 cgtcacatat cagaccgatc agcaaacctt gatcctcaac accgcgaccg cttatttcaa
    21181 cgtgttgaat gctattgacg ttctttccta tacacaggca caaaaagaag cgatctaccg
    21241 tcaattagat caaaccaccc aacgttttaa cgtgggcctg gtagcgatca ccgacgtgca
    21301 gaacgcccgc gcgcagtacg ataccgtgct ggcgaacgaa gtgaccgcac gtaataacct
    21361 tgataacgcg gtagagcagc tgcgccagat caccggtaac tactatccgg aactggcggc
    21421 gctgaatgtc gaaaacttta aaaccgacaa accacagccg gttaacgcgc tgctgaaaga
    21481 agccgaaaaa cgcaacctgt cgctgttaca ggcacgcttg agccaggacc tggcgcgcga
    21541 gcaaattcgc caggcgcagg atggtcactt accgactctg gatttaacgg cttctaccgg
    21601 gatttctgac acctcttata gcggttcgaa aacccgtggt gccgctggta cccagtatga
    21661 cgatagcaat atgggccaga acaaagttgg cctgagcttc tcgctgccga tttatcaggg
    21721 cggaatggtt aactcgcagg tgaaacaggc acagtacaac tttgttggtg ccagtgagca
    21781 actggaaagc gcacatcgta gcgtcgtgca aaccgtacgt tcctccttca acaacattaa
    21841 tgcttctatc agtagtatta acgcctacaa acaagccgta gtttccgcgc aaagctcatt
    21901 agacgcgatg gaagcgggct actcggtcgg tacgcgtacc attgttgatg tgttggatgc
    21961 gaccaccacg ctgtacaacg ccaagcaaga gctggcgaat gcgcgttata actacctgat
    22021 taatcagctg aatattaagt cagccctggg tacgttgaac gagcaggatt tgctggcact
    22081 gaacaatgcg ctgagcaaac cggtttccac taatccggaa aacgttgccc cgcaaacgcc
    22141 ggaacagaat gctattgctg atggttatgc gcctgatagc ccggcacccg tcgttcagca
    22201 aacatccgca cgcactacca ccagtaacgg tcataaccct ttccgtaact gatgacgacg
    22261 acggggcttc ggccccgtct gaacgtaagg caacgtaaag atacgggtta tctgccgcat
    22321 tcttccccct tctcgcttca atttcgacca gccatcctct attctgatgg gtatttacca
    22381 ctggtcccgg aagacaaaaa tgaaacggac aaaatccata cgccacgcat cgttccgcaa
    22441 aaactggagc gcacgccatc tgacaccagt cgctctcgcg gttgccactg tttttatgct
    22501 ggctggctgt gaaaagagtg atgaaacagt gtctctctat caaaatgctg acgactgttc
    22561 agctgcaaac ccaggcaaaa gcgccgaatg taccaccgcg tacaacaatg cgctgaaaga
    22621 agccgaacgt actgcgccga aatacgccac ccgtgaagac tgtgttgctg aatttggtga
    22681 aggtcagtgt cagcaagcac cagcccaggc tggcatggca ccagaaaacc aggcgcaggc
    22741 tcagcaatcc agcgggagtt tctggatgcc gctgatggcc ggttacatga tggggcgtct
    22801 gatgggcggc ggcgcgggat ttgcacagca gccgctgttc tcctcgaaaa acccggccag
    22861 tccggcttac ggtaaatata ccgacgcgac gggtaaaaac tatggcgcag cccagccagg
    22921 ccgcaccatg accgtaccga agacggcaat ggcaccaaaa ccggcgacca ccactaccgt
    22981 tacccgtggc ggttttggtg aatctgttgc caaacaaagc actatgcagc gtagcgcaac
    23041 cggcacctct tctcgttcaa tgggtggctg ataccgatgg aaagagtcag tattaccgag
    23101 cgcccggact ggcgtgagaa agcccacgaa tacggtttca attttcacac catgtacggc
    23161 gagccgtact ggtgtgaaga tgcttactac aagttgaccc tcgcccaggt tgaaaagctg
    23221 gaagaagtca ccgccgaact gcaccagatg tgcctgaaag tggtggaaaa agtgatcgcc
    23281 agcgatgagc tgatgaccaa attccgcata ccaaaacaca cctggagttt tgtgcgtcaa
    23341 tcatggctga ctcaccagcc atcgctttat tcgcgtcttg atctggcgtg ggatggcact
    23401 ggcgaaccta aacttctgga aaataacgcc gatacgccaa cgtcactata cgaggcggcg
    23461 ttctttcagt ggatctggct ggaagatcag cttaacgccg gtaacttgcc ggagggcagc
    23521 gaccagttta acagtctgca agaaaaactg atcgatcgct tcgttgagct gcgtgaacag
    23581 tgtggcttcc agttgctgca tctcacctgc tgtcgcgaca cggtggaaga tcgcggaacc
    23641 attcagtatt tgcaggactg cgcaaccgaa gctgaaatcg ccaccgagtt cctctacatc
    23701 gacgagatag gtttaggcga aaaaggccag ttcacggatt tacaggatca ggtgatttct
    23761 aacctgttca agctttatcc gtgggaattt atgctgcgcg aaatgttctc caccaagctg
    23821 gaagatgctg gcgtacgctg gctggaaccg gcgtggaaga gtattatctc caacaaggca
    23881 cttctaccgc tactgtggga gatgttcccg aatcacccga acctgctgcc cgcttatttt
    23941 gcggaagatg atcatccgca aatggaaaaa tatgtggtta aaccgatctt ctcccgtgaa
    24001 ggcgcaaacg tgtcgatcat tgagaacggc aaaaccattg aagcagcgga aggtccgtat
    24061 ggcgaagaag ggatgattgt tcagcaattc cacccgttac cgaaattcgg cgacagctat
    24121 atgctgattg gtagctggct ggtgaacgat caacctgccg gaattggcat tcgtgaagac
    24181 cgtgcgttga tcacccagga tatgtctcgg ttttatccac atatttttgt tgaataagcc
    24241 acgataccgg atggcactcg ccatccggta attgttagcc tatctgcacc gacagcatac
    24301 ttaaactgcc catttctata ccatcgaccg gaatggtaat tggctcctgc ccatcccaca
    24361 cacctaacac atacaacaac ggcagatagt gctctggcgt tgggttcgat aacgcgccac
    24421 cttcatggtc gagataattc accagaggat gctgctctac cggcccttgc cacgtcagat
    24481 tcgctttcac atactcatta aacgacgtcg cccacggata cggtgaacta tcaccgtgcc
    24541 acttcactgt gcgcaggtta tgcaccacgt taccgctggc gaccaacatt attccttcat
    24601 cacgcaacgc cgcgagtttg cgccccattt cgaaatgcca ggcggcaggt ttgctactgt
    24661 cgatacccaa ctgcaccata gggatatcgg cgtcaggata catcttaatc agcacgcccc
    24721 acgagccgtg gtcaaagccc caggcttctt tatccagcgt caccgggatc ggcgctaaca
    24781 gctcaaccag acgctgtgcc agcgcaggcg aacccggagc aggataatgc gtatcgtaca
    24841 gcgcctgcgg gaagccacca aagtcatgaa tcgtgggcgg cgtctccatc gcggtcactc
    24901 ctgttccacg ggtaaaccag tgagccgaaa ccaccacaat cgcttgcggg cgtggcaatg
    24961 tcatccccaa cttctgccag ctgcgggtat acaaattatc ttccagcacg ttcatcggac
    25021 taccgtgacc taaaaacaat gctggcatac gtgttgaaga catgatgata tccttaacta
    25081 aaggtgtcat tttgatatcc tcacaatacg cttgttcggc ggagtaagaa cccggataac
    25141 aatgatgatg atcatcagtt attttgacga tctgcctgaa ggtgaagatt tataaggagt
    25201 tgtcgatgtc agtacctctc attctgacca tactggcggg ggcagccacg tttattggcg
    25261 cgtttctcgg cgttctcgga caaaaaccct cgaaccgctt actggcgttt tcgctaggtt
    25321 ttgcggcggg gatcatgttg ctcatctcat taatggaaat gcttcctgcc gcactagcag
    25381 ctgaaggaat gtcgccggtg ttgggttatg gaatgtttat cttcggtctg cttggctact
    25441 ttggcctgga ccgcatgttg ccgcatgcgc atccgcagga tttaatgcaa aaatcggtgc
    25501 agctgttgcc aaaatcgatc aagcgcacag ccattctgct cactctcggc atcagtctgc
    25561 ataacttccc ggaagggatt gccacctttg tcacggcgag cagcaacctg gagctgggat
    25621 ttggcatcgc actggccgtc gcgttgcaca atatccctga aggtctggca gtggcaggcc
    25681 cggtttatgc ggcaacgggg tctaaacgca ccgcgattct gtgggcgggg atttctggac
    25741 tagcagaaat tcttggtggt gtgctggcgt ggttaatcct cggtagcatg atttccccgg
    25801 tggtcatggc ggcaatcatg gcggcggttg caggaattat ggtggcgctc tcggttgatg
    25861 aattaatgcc gctcgccaaa gagattgacc ctaataataa ccccagctat ggcgtactat
    25921 gtggaatgtc agtgatggga ttcagtttag tgctgctaca aacggcggga attggttaaa
    25981 aacaacatca gaagttgtcc agcacatgga caacttctgc cagcttcacg aaagccttat
    26041 tctacctttt cggctgtctc ttctctcgta ctgtttaagt atttgttccg cgcttcaagc
    26101 agtgcgtctc tgtcctggcg aaacgcccga caataacgtt tcaaatgcag gaaataaccc
    26161 accacaacca gagcaatcaa tacaatccag taccaggcga taaacatcct tttaccctta
    26221 atatttaaca atactaaaca tttagcgcat aaatttcaca tatccttttt cggatatatt
    26281 catatggtcg cataataacg gaacaattat atgattttga tcataatgaa aaagaatgcc
    26341 tgatattaat gaaattttct catagaaaga gaagcggaat taacatcttg catagcaggg
    26401 caaaataaaa accgaagcca gctacaactt cggtttcttg attaaggcag taattaagca
    26461 gcggttttca gctggcttta cgctcatgtg cctgacggta tgccaccagg tcttcaatag
    26521 tcacgagcgc catattgtgt ttattggcaa actcaatgca ctctggtgca cgcgccatcg
    26581 tgccatcgtc attagtcagc tcacacagca caccagccgg tttaaagcct gccagcgtca
    26641 tcaaatcaat agtcgcttca gtatgaccgc cacgcgtcag tacaccacct gcctgcgcgc
    26701 gaagtgggaa aacgtggccc gggcgattca gatcagaagg ttttgcgcca tcggcaatcg
    26761 ctgcgcgaac ggtcgtaata cgatcagcgg cagaaacacc ggtagtcaca ccttcagccg
    26821 cttcaatggt cacggtaaaa ccggtgccat aggcgctggt gttattttct accatcattg
    26881 gcagatcaag ttgtttacgg cgatcttcag tgatgcacag gcaaacaata ccgctaccgt
    26941 ggcgaatggt cagcgccatc tgctcaacgg tcatggtttc tgccgggaag atcatatcgc
    27001 cttcgttttc acggtcttca tcatcaagca ccattacacc gcgtccttca cgcagcgcag
    27061 ccagtgcatt ttcaacacgt tcgaaaggcg taccaaaaga ggaaagtagc gtctgattca
    27121 tggtaaaaaa aacctcacta aaattatggt taccagaatc agggcagtct taggagtggc
    27181 ggcatatagc caaaataacg tgagcgggtc catgcccgac agaatcgtta ctctctccca
    27241 tccggactct aaccgtcggc cccggaatta caccggatct gctgtccttt gagttcgcac
    27301 ccaaagcgct cgcgggcttt caactgagtt gatttaccgc cggtggggaa tttcgccccg
    27361 ccctgagaat aagcggattc actataactc taatgattag cggcagcaac gcatagcttc
    27421 acataattct ggtttatgac ttacccttat cgcactacaa tggtactcaa cacctatcag
    27481 tacagggaaa ctacaatgat tgacccgaaa aaaattgagc aaatcgctcg ccaggttcac
    27541 gaatcaatgc ctaaaggaat cagggagttc ggggaagatg tggagaaaaa aatccgccaa
    27601 accctacaag ctcagctgac gcgcctcgac cttgtaagcc gtgaagagtt cgacgtccaa
    27661 acgcaagtcc tgttacggac tcgtgagaaa ctggcgctgc ttgaacaaag aatcagcgaa
    27721 ctggaaaatc gcagtactga gatcaaaaag cagccagatc cagagactct ccccccaacg
    27781 ctgtaaacat ttgtctttaa tgcactgtaa attatgaatt aatcctctaa tttacagtgt
    27841 tttctattac attatcgctt aatcc
//
LOCUS       DAJTYG010000048        27148 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTYG010000048 DAJTYG010000000
VERSION     DAJTYG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.048"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            282..1580
                     /gene="kgtP"
                     /locus_tag="OO568_002205"
     CDS             282..1580
                     /gene="kgtP"
                     /locus_tag="OO568_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6641546.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1577..1900)
                     /locus_tag="OO568_002206"
     CDS             complement(1577..1900)
                     /locus_tag="OO568_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6641547.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1946..3301)
                     /gene="pssA"
                     /locus_tag="OO568_002207"
     CDS             complement(1946..3301)
                     /gene="pssA"
                     /locus_tag="OO568_002207"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6641548.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3415..6075)
                     /gene="pat"
                     /locus_tag="OO568_002208"
     CDS             complement(3415..6075)
                     /gene="pat"
                     /locus_tag="OO568_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6641549.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6107..6805)
                     /gene="tapT"
                     /locus_tag="OO568_002209"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6107..6805)
                     /gene="tapT"
                     /locus_tag="OO568_002209"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6641550.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGVAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6874..7293)
                     /gene="trxC"
                     /locus_tag="OO568_002210"
     CDS             complement(6874..7293)
                     /gene="trxC"
                     /locus_tag="OO568_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6641551.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7500..8537
                     /locus_tag="OO568_002211"
     CDS             7500..8537
                     /locus_tag="OO568_002211"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6641552.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8585..9274)
                     /gene="ung"
                     /locus_tag="OO568_002212"
     CDS             complement(8585..9274)
                     /gene="ung"
                     /locus_tag="OO568_002212"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6641553.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9579..9962
                     /gene="grcA"
                     /locus_tag="OO568_002213"
     CDS             9579..9962
                     /gene="grcA"
                     /locus_tag="OO568_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6641554.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10018..10605)
                     /gene="eamB"
                     /locus_tag="OO568_002214"
     CDS             complement(10018..10605)
                     /gene="eamB"
                     /locus_tag="OO568_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6641555.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10708..11589
                     /locus_tag="OO568_002215"
     CDS             10708..11589
                     /locus_tag="OO568_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6641556.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11624..12958)
                     /gene="srmB"
                     /locus_tag="OO568_002216"
     CDS             complement(11624..12958)
                     /gene="srmB"
                     /locus_tag="OO568_002216"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6641557.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13090..13827
                     /gene="trmN"
                     /locus_tag="OO568_002217"
     CDS             13090..13827
                     /gene="trmN"
                     /locus_tag="OO568_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6641558.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13812..15434)
                     /gene="nadB"
                     /locus_tag="OO568_002218"
     CDS             complement(13812..15434)
                     /gene="nadB"
                     /locus_tag="OO568_002218"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCR6641559.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLISGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15690..15845
                     /gene="rseD"
                     /locus_tag="OO568_002219"
     CDS             15690..15845
                     /gene="rseD"
                     /locus_tag="OO568_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6641560.1"
                     /translation="MLRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15842..16417
                     /gene="rpoE"
                     /locus_tag="OO568_002220"
     CDS             15842..16417
                     /gene="rpoE"
                     /locus_tag="OO568_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6641561.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIEFLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16450..17100
                     /gene="rseA"
                     /locus_tag="OO568_002221"
     CDS             16450..17100
                     /gene="rseA"
                     /locus_tag="OO568_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6641562.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17100..18056
                     /gene="rseB"
                     /locus_tag="OO568_002222"
     CDS             17100..18056
                     /gene="rseB"
                     /locus_tag="OO568_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6641563.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHACLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18053..18532
                     /gene="rseC"
                     /locus_tag="OO568_002223"
     CDS             18053..18532
                     /gene="rseC"
                     /locus_tag="OO568_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6641564.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18730..20529
                     /gene="lepA"
                     /locus_tag="OO568_002224"
     CDS             18730..20529
                     /gene="lepA"
                     /locus_tag="OO568_002224"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6641565.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20545..21519
                     /gene="lepB"
                     /locus_tag="OO568_002225"
     CDS             20545..21519
                     /gene="lepB"
                     /locus_tag="OO568_002225"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6641566.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21791..22471
                     /gene="rnc"
                     /locus_tag="OO568_002226"
     CDS             21791..22471
                     /gene="rnc"
                     /locus_tag="OO568_002226"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6641567.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22468..23373
                     /gene="era"
                     /locus_tag="OO568_002227"
     CDS             22468..23373
                     /gene="era"
                     /locus_tag="OO568_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6641568.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDIQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23385..24113
                     /gene="recO"
                     /locus_tag="OO568_002228"
     CDS             23385..24113
                     /gene="recO"
                     /locus_tag="OO568_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6641569.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24125..24856
                     /gene="pdxJ"
                     /locus_tag="OO568_002229"
     CDS             24125..24856
                     /gene="pdxJ"
                     /locus_tag="OO568_002229"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6641570.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24856..25236
                     /locus_tag="OO568_002230"
     CDS             24856..25236
                     /locus_tag="OO568_002230"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6641571.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25656..25736
                     /gene="shoB"
                     /locus_tag="OO568_002231"
     CDS             25656..25736
                     /gene="shoB"
                     /locus_tag="OO568_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6641572.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25930..26190)
                     /gene="yfhL"
                     /locus_tag="OO568_002232"
     CDS             complement(25930..26190)
                     /gene="yfhL"
                     /locus_tag="OO568_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6641573.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26405..>27148
                     /locus_tag="OO568_002233"
     CDS             26405..>27148
                     /locus_tag="OO568_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR6641574.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSFRVQKPTPTRLFTDIESIARLWLNVA"
BASE COUNT         6696 a         6765 c         7113 g         6574 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct tatactccca catatgacag
       61 attcagcaac ggatgcggtt tccccaactt gcctacttcc atacgtgtcc tccttaccag
      121 aaatttatcc ttaagctcct caataaccat tttcctgcta actaaattca tggtttaggt
      181 tgcataatga tacgcaacaa atgtataaca ttttatttac caaaagaaat aaacaaaagc
      241 gaccaacaaa agcatcggat tacggcagga gacataatgg catggctgaa agtactgtaa
      301 cggcagacag caaactgaca agtagtgata ctcgtcgccg catttgggcg attgtggggg
      361 cctcttcagg taatctggtc gagtggttcg atttctatgt ctactcgttc tgttcactct
      421 acttcgccca cattttcttc ccttccggga acacgacgac tcaactacta caaacagcag
      481 gtgtttttgc tgcgggattc ctgatgcgcc caataggcgg ttggctattt ggccgcatag
      541 ccgataaaca tggtcgcaaa aaatcgatgc tgttatcggt gtgtatgatg tgtttcggat
      601 cgctggttat cgcctgcctc ccaggttatg aaactatagg tacgtgggct ccggcattat
      661 tgcttctcgc tcgtttattt cagggattat ccgttggcgg agaatatggc accagcgcca
      721 cctatatgag tgaagttgcc gttgaagggc gcaaaggttt ttacgcatca tttcagtatg
      781 tgacgttgat cggcggacaa ctgctagccc tactggttgt cgtggtttta caacacacca
      841 tggaagacgc tgcactcaga gagtggggat ggcgtattcc tttcgcgtta ggagctgtgt
      901 tagctgttgt ggcgttgtgg ttacgtcgtc aattagatga aacttcgcaa caagaaacgc
      961 gcgctttaaa agaagctgga tctctgaaag gattatggcg caatcgccgt gcattcatca
     1021 tggttctcgg ttttaccgct gcgggctccc tttgtttcta taccttcacc acttatatgc
     1081 agaagtatct ggtaaatact gcgggaatgc atgccaacgt ggcgagtggc attatgactg
     1141 ccgcattgtt tgtattcatg cttattcaac cactcattgg cgcgctgtcg gataagattg
     1201 gtcgccgtac ctcaatgtta tgtttcggtt cgctggcagc catttttacc gttcctattc
     1261 tctcagcatt gcaaaacgtt tcctcgcctt atgccgcttt tggtctggtg atgtgtgccc
     1321 tgctgatagt gagtttttat acatcaatca gtggaatact gaaggctgag atgttcccgg
     1381 cacaggttcg cgcattaggc gttggtctgt catatgcggt cgctaatgct atatttggtg
     1441 gttcggcgga gtacgtagcg ttgtcgctga aatcaatagg aatggaaaca gccttcttct
     1501 ggtatgtgac cttgatggcc gtggtggcgt ttctggtttc tttgatgcta catcgcaaag
     1561 ggaaggggat gcgtctttag tgacgggtca gttgccagac ggtatagccg gtgcttgcac
     1621 cggcgacatc ccaggccaaa tccttccagc tccagccgct cccttcgggg cggctatccc
     1681 aaagctcttt tgacgccccc aaactgacag agaacatcaa tccaaacatg gcactgcgat
     1741 cccggctcat cccctgatgc tgtgaatatt catttccggc ggcggaaagt atcgccgagg
     1801 cgataaagtg ttgagcttta tcctgcccgc tccagctatc gttagccata tggctgcatc
     1861 cagaaagtaa caatagcgaa cagacaaaaa gaatacgcaa aacgtggcct ccaaaaaaca
     1921 aaccccgtac aggacggggt tgtgattaca ggatgcggct aattaatcgg tcgatgcgga
     1981 tacggcgcaa acggcggatg agtttacgaa ccttcaccgg ataatcggca atactttgca
     2041 gatcgcgata gtgcttaacg atggtggtac gctcgcggat cagctccagt tctttctctc
     2101 gctgcggcgc aagctccagt tgtggatcgt ggatcaaaat ggcgttttcc agatccagac
     2161 gccaggcgcg cgggttcagg ttattaccgg tgatcaacat ccacttatca tcaacccaca
     2221 tccctttcag gtgataggtg ttgtcgtcat ctttccataa ccgaaccact agctggtcag
     2281 tattgacgta atactgcaaa cggctcagga aacgacgcag attgatctca tagagataag
     2341 gcaatgcgcc aattatcttg aaaggttcat cttccggaat gtagaagtcg ttcgcggttt
     2401 tatcaccaac aataatttcg acctttttcc cttcgcgcag caactggata atattgcgca
     2461 caaggattgc tggcaggttg aagtatggcg tacagatggt tagtttctgc tcggcacaag
     2521 gcataagatg gaaaatggtc ttgttcaaca gactcgattt ccccagcccc actagcggcg
     2581 ttacggaaag ctgatcgttg tcggcatcgc cctggaaatg ataagcggca tcacgcagct
     2641 cctggcggaa cagacgaata tcgttcttga tttccgggct ttttggccga ttaacatcat
     2701 ccagacgatt aacgccgcgg ccattcataa tattctgtgt aacccattca aacataatgt
     2761 ctgacatctt acggttacgg atcagatgat aacggtcgta gcgatattta tcgtgttgat
     2821 gcaggtaaac atcgttcagg ctggcaccgc tataaagtac gctatcgtcg atgataaagc
     2881 ctttaaagtg cagaacacca agagcttcac gggtattgat tggaacgcca taaaccggaa
     2941 catctacgcc cggattttcc tgcgccatgc ggcagtacca gtcagcgtta gtgttagatg
     3001 ccgcagcgcc aatgcgtcca cgttgtgcac gatgccagtc gaccagcacc cgcacatcca
     3061 gttccggacg ctgcctttta gcctcataca acgcgttcag aatgcctttg ccaccgtcat
     3121 cctgttcgag atacagggcg acaatgcaaa tgcgctgctt cgcgctggct attttttcca
     3181 gcagcgtctc ccggaagtcg gcgggagcgt aaaagaaatc gacatcatca actgattgag
     3241 aaatcttggg tagttgggca aggtgttgtt gatgtttatt acgcttaaat tttgacaaca
     3301 tcacagtgca tttcttctct gttcattgaa gggtcctctg tgcaatgcag acgacataag
     3361 cgggcaataa taacaccagt cccgattaag tggtcaacat ttccagtacc ttactcatga
     3421 ttcctcgcgc cgggcaagat ttagcgtaag cccaacgatc ccctcttcga gctggatatc
     3481 aacgttaaac ccgagcttgc gggctagcgc caccatgcca cgattgtttg gcatcgtaat
     3541 accattcaga cgttgtagtc cgtgatttcg cgtataggta atcaactttt ccattaagcg
     3601 tcgacctaag cctaaccctt tgagatccga gcgaaccagt acagcaaatt cggcatcgat
     3661 gttatcagga tcggaaatcg cacgcgtgac gccgaggatc tcttccgttt gatcaatacg
     3721 tcgtaccgct acaaacgcca tttcccgatc gtagtcgatc tgtgtcatgt tggctaaatc
     3781 ttcatgggta aattcgttga tctcgctaaa gtagcggtaa taaagatctt ctttggtgac
     3841 tcgcgaaatg aattgctgaa gttgtggctc atcttctggc aaaatcgggc ggaacaagca
     3901 gcgttcaccg tttttcaatt ctacccattc ttccagctga tgcggataag ggcgcactgc
     3961 cagccgactc tcgttatcgc cttcaaacgg cgagatatcc agcgtgacat ccagcgcggt
     4021 aaattcactg ccagaagcca gcaaaggatg aatatccaga cgctgaattt ccgggcaatc
     4081 gacaatcaag ttggaaacct gcaccagaag ctggctcaag cctgcaacat ccaatgggcg
     4141 tagcgcactg cgcgcacgaa tctttttact tttgatcccc tgaataacca gatagcgggc
     4201 caggttcatg ttcagcggcg gcagtgcgac gacggcttga tcttcaggac gccactccac
     4261 accgccttca cccagcatga tcaacggccc gaaaaccgga tcgtgctcaa ccacaacccg
     4321 caactcctga gcgccagcac ggttagccat actttgcacc aacaggccgt ggacccgcgc
     4381 ctgtggccag gccattttta cgcgatcgaa aatagcgttc gccgcttgct ggacttcatt
     4441 ggctgtacgc aggtaaagca tgacgccctg aacttccgat ttatgtggaa tatccggcga
     4501 acgcaatttc agcgccaccg gataaccaat ctgttcggca atatgcaccg cttcggtgct
     4561 atcgctggca atccaggttg ggagcgtgtt catgccatac gcttgcagga tgggctgaac
     4621 ttcatgggta tcgagcgacg tagccccttc ggcaatcgcc tgttgcaaca gaagatgcgc
     4681 ttctgcggta ttggaagtca gattgctggg caacgccggc gtttcgcgta gttgcttctg
     4741 attacgccgg tactccacca tatgcataaa agcagtgatg gttccttccg gggtacggta
     4801 ggtcggcagc ccggcttcgc tgaataaacg tcgtgcctct tgcgaggagt gctcgccgca
     4861 ccagttcgtc agcaaagaga catatttgct gcggggatga tgctttaccg cttcaattaa
     4921 tacttgcgcg ctttctgttg cgggagcagc ggcgctgggc gaatgaataa ccatcagcgc
     4981 atcaaaatcc tggctgtgga gcagaatgtc cagcgttttg acataatgct cgctgctggc
     5041 gtcatcgcgc agatcgagcg ggttagagac tgccacatgt tctggcaatg catcgcgcaa
     5101 tttctggcag gtttcttcgc ttagcgttgc cagcttgcca ttgcgtgacc ataaggcatc
     5161 cagcgccagc gcggcaggcg cagcaccgtt gctgataatc atcaaccggt cgccacgcag
     5221 cgggcgcata tggctaaggg tttccaccgc cgaaaacagt tcgtgggtat cctgtacccg
     5281 caacaaaccg gcacgctgaa tagccgcatc ccatgccggg tccattcctg ccgtggtgtt
     5341 gagcagtcgc tgtgccgccg ggctacgtcc gcttttaatc accagaatcg gtttattacg
     5401 cgaggcacta cgggccgccg aaacaaagcg tcgcgcgtcg cttaactgtt cgagatagag
     5461 caggatggcg ctggttttac tgtcgcgcgc cagatagtcg agcaattcat caacgtcgat
     5521 atccaggctg tcgccgagcg caataaagta ggaaaagccc atctcacgct gttgcgccca
     5581 gtcgaggatg gtgttggaga cggcagccga ttgcgaaata aacgccagct tgccgcgttt
     5641 aatcggcaca ggcgaaaagc tggcattcag accttgccag ggagccagta aacccagact
     5701 gtttggtcca agcaggcgca tgttatggcg cagggcgcag gcgcggagat cttcgtgttg
     5761 cgatgccggg gcggaaagaa taatgcaggt tttacagcct ttctcgccga gctcttccag
     5821 aagagcaaga ttacggctgg cattggtaca taaaaccgca aggtcgggtg taaagggcaa
     5881 gctggcaata tccggccagg ccaacacacc caacaccgct ttccaggctg gcgtcaccgg
     5941 gagtaccggt ccgttaaagc ctcccgccag caggttacgc atcatcaggt aacctgcgcg
     6001 attgggtttc atcgacgcgc caattaccgc tatcgatttt ggtcgcagta gtgcttccag
     6061 tcctcgctga ctcataccgg tctcccgcag aagtgaccga atgattttaa acgctttcta
     6121 actgttctgc tgtgatgcta cccagatgtt gcgtttttcc tgccagatag cgtgttttaa
     6181 agcgggtaaa atgctcgcct aaccctgctg ccaccccggt atcgccggcc atatctaaca
     6241 gtgcgatggc tacctcggca gtacaatatt ggcctgcagc ctgggcttca cgcaggcgat
     6301 aggcagaaag ccgggaaaga tcgacggaaa tgacgggaag attatccaga tacggacttt
     6361 tacgaaacat cttgcgagct tccggccagg taccatcgag catgataaac agcggtggct
     6421 taccggcagg tggtgtgaag atcacttccc gttgctcatc agcatacgag gcgggaaaga
     6481 ccaccattgg ctgataatcc gggttttgta ccagatccag caaatcctgc gagggttcgg
     6541 tacgcgacca ttgaaacgca acggtatcag gcaaaatatc agcaatcaga cgcccggtat
     6601 tactgggctt cattggctcg gtgtcgaaca tcagcaaaca gaagcgactt tttgcttgtg
     6661 ctggggtaat tgtcgaacag agacataatt tctctggcaa aaggcagcgt tggcagcgac
     6721 gaacgcgatt accgcgggca agaaaaggac gtgttgcgcg cgcaatacgc tcggcgcgta
     6781 actggagaac agcgttttcg gtcataagag agcgtcgaaa aaacgccatt gtcgcagagg
     6841 agaaaacggg gcacaagatg cgccccggta agattaaaga gattcgttca gccagctatc
     6901 gaacggcgct ttcggtactg cgccattaag catgtcgaca acctgaccgt ttttgaaaat
     6961 catgatcgtc ggtatactac gaattccaaa gcgactgctc aattcacgtt cagcttcggt
     7021 attcactttc acaaagcgca ctttaccgct acgctcttgc gcgacatctt caaaaattgg
     7081 tgcgaaatta cggcaggggc cgcaccacgg tgcccagaag tcgatcacca caggtagatc
     7141 atccttcagc aatttgtcga gtgtttcacc ggtcgcatta atcacctctc cgtcaaacaa
     7201 gtcgtgaccg cagcgtccgc attttgccgc atcttcgatc cgatcgtcgg gaatgcgatt
     7261 gatggcctga caatgggtac aaacggtatt cataactaac ctcgggatga gtaaggagac
     7321 ttatcggcaa cgacgccggt atgtttctaa tatgttacat attatcgggg acattgttcc
     7381 agacaacaat ctgttttatt caataggtat gatagtcgca ggctttggta cgaaatcatg
     7441 gcgaaccgcg cggacttcgg gtaatctgcg cgcttcgcgc agcgctggtg gagaaaagca
     7501 tgaacgatga aatgaaaggt aaaagcggca aggtcaaagt gatgtatgtc cgcagtgatg
     7561 atgattctga taaacgtacc cacaacccgc gtaccgggaa agggggcggg cgtccaggaa
     7621 aatctcgtgc tgatggtggc cgtcgccccg cccgcgatga caaacagagt cagccccgtg
     7681 accgcaagtg ggaagattcg ccgtggcgca cggtttcccg cgcgccgggt gatgagacgc
     7741 cggaaaaggc cgatcacggt ggcatcagtg gtaaaagttt tattgatccg gaagtgttgc
     7801 gtcgtcagcg tgcggaagaa acccgcgtct acggcgaaaa cgcctgtcag gcactgttcc
     7861 agagccgtcc ggaagcgatt gttcgcgcct ggtttatcca gagtgtaacg ccgcgtttta
     7921 aagaagcctt gcgctggatg gcagcaaacc gcaaagcgta ccatgtggtg gatgaagcgg
     7981 aattgacaaa agcgtcaggc acggaacatc acggcggcgt ttgcttcctg atcaaaaagc
     8041 gtaacggtac aaccgtgcag cagtgggtaa tccaggcagg cgcgcaggat tgcgttctgg
     8101 cactggaaaa cgaatctaac ccgcataacc tgggtggcat gatgcgcagc tgcgcgcact
     8161 tcggcgtgaa aggtgtcgtg gtgcaggatg cggcactgct ggagtcgggg gcggctatcc
     8221 gtaccgcaga aggcggcgca gagcacgttc agccgattac tggcgacaac attgttaacg
     8281 tgctggatga tttccgtcag gcaggttaca ccgtagtgac cacttccagc gagcagggta
     8341 aaccgctgtt caaaaccagt ctgccagcga aaatggtact ggtgctgggt caggaatatg
     8401 aagggttacc ggatgccgca cgcgatccga acgatctgcg cgtgaagatt gacggtacgg
     8461 gcaacgttgc cgggctgaat atctctgtcg caaccggcgt tttgcttggt gaatggtggc
     8521 gtcagaataa agcctgataa atcagccggg tggcaactct gccatccggc atttccccgc
     8581 aaatttactc actctctgcc ggtaatactg gcatccagtc aatcggcgtc tcgccacgtt
     8641 gttccagcca ctgatttgcc agcacaaaat ggttgcagcc aaagaatcca cgatgcgccg
     8701 aaagcggcga cggatgcggt gctttcagta catgatggcg ttgcttatct ataatcgccc
     8761 ctttcttttg cgcatgtgat ccccacaaca aaaacaccac gccttcgcgg tgctggttaa
     8821 tcaggctgat gactttatca gtaaacgtct cccaaccgag gctggcgtgg gaatgcgcct
     8881 gacctgcgcg aaccgtcaac acagtattga gcagcagaac gccctgacgc gcccagcttt
     8941 cgagatagcc atgattaggg cgggtgaagc ccggaatagt attttccagc tctttataca
     9001 tattcaataa tgacggagga atggcaatgc cgggacgaac ggaaaatgcc agaccatgcg
     9061 cctgtcccgg tccgtgataa ggatcctggc cgagaatcac cactttaacg tcacccaact
     9121 cggtaaagcg gaacgcgtta aagacatctt tttgtggtgg atagatagtg acgccggact
     9181 gccgctcgct ggcgacggtc tgaagggtat taagaaaata aggttgttgc ttctcttcag
     9241 ccagcacgtc atgccaggtt aattcgttag ccatcttgct ctcctgcgaa tcttcaatcc
     9301 gcctagctta actgcttctt tctacagaac aaaatcatgc tcatttttta gacttaaacg
     9361 agtgcaaaaa aatgtaaatt ggtaaaattt cacacccgca taagttgatg taaaacaata
     9421 aaatccattt acgaccacaa ttgatgtgtg gtttttgttg atttaaatca aagaatcaag
     9481 ggtggttgag gagtatatat acactcaagc aacaatggtt ttaccaattg gccgcgacag
     9541 gctgaacaaa tcaaataatt ttgccgggga ggcatcacat gattacaggt atccagatta
     9601 ctaaagccgc taacgacgat ctgctgaact ctttctggct gctggacagc gaaaaaggcg
     9661 aagcacgttg catcgttgca aaagcaggtt atgcagaaga tgaagtggtt gcagtaagca
     9721 aactgggtga cattgaatac cgtgaagttc cagtagaagt gaaaccagaa gttcgcgttg
     9781 aaggtggtca acacctgaac gttaacgttc tgcgtcgtga aactctggaa gatgcagtta
     9841 agcatccgga aaaatatccg cagctgacca tccgtgtatc cggttatgca gttcgcttta
     9901 actctctgac tccggaacag cagcgcgacg ttatcgctcg tacctttact gaaagcctgt
     9961 aaggcttaag tagcaagtta ctaagcggaa gagggcgacc tcttccgctt tttttcgtta
    10021 atagaaaatg cgtaccgcgc aatagaccag caacagggca agcacgatat ttaactggcg
    10081 accatactgg cgaaacaatc gctgaaacag atgccccgcc agcgcccagc acacattgcc
    10141 aaacgtccca atcatcgcca gcaaaacgct gacgccaact acccagctta gcgcctgtgt
    10201 ttgcggcaga acaaacgtcg acagtgccgt aacaccgtac aaaatgattt tgacgttcac
    10261 aaactgcaaa gcaaagctgg cccaaaagct gattggtttt gcctgaagtc cgtcttcctt
    10321 tgttgggctg gtggcgattt tccacgccag ccagacaata tatgccgccc ccgcccaact
    10381 caaaaggtgt accgctgccg ggtcaatcac tgccagtgaa aatgaaatgc ccgcacacag
    10441 taacatcaca atcaaaaatc ccagactcat ccctgccagc acgcgggtac tttgacgaaa
    10501 tccatgcgac gtagcagagc taagggcgag aatattgttc ggtcctggcg tcatagcggt
    10561 aatcagggtg taagtccaaa aagcacttaa aaggatcggt gtcacttgtt atctcctctg
    10621 ttttggtaga cagtacccga tacggaacat ttaaaaaaat gaatgttttt atattctaaa
    10681 ttcgatattt tcgataggtt tggggttatg gatctgcgcc gttttattac gcttaaaacc
    10741 gtggtggaag agggttcctt tttgcgagct tcgcaaaaat tgtgctgtac acaatcgacg
    10801 gtgacttttc atattcagca gcttgagcag gagttttcag tccagttatt tgagaaaatt
    10861 ggccgacgga tgtgccttac ccgcgaagga aaaaagttgc tgccgcacat ttatgaactt
    10921 acccgagtga tggatacgct tcgtgaggct gccaaaaagg agtcagatcc ggacggggaa
    10981 ctacgcgttg tttcgggcga aacgttgctc tcttaccgaa tgccgcaggt gttgcagcgt
    11041 tttcggcaac gcgcaccaaa agtgcgttta tcgttgcagg cgctgaactg ttacgtgatc
    11101 cgtgatgcgc tactgaatga tgaggccgat gtcggcgtct tttatcgcgt agggaatgat
    11161 gatgccctga accgacgaga gttgggtgaa caatcgctgg tgctggtggc ttcaccacaa
    11221 attgcagatg tcgattttac tgagccggga agacataacg cctgtagctt tattatcaac
    11281 gaaccgcaat gtgtcttccg gcagatattt gagagcacgc tgcgacagcg gcggatcacg
    11341 gtggaaaaca ccattgagct tataagtatc gaaagcatca agcgttgtgt cgcggcgaat
    11401 atcggtgtca gttacttgcc ccactttgcg gtggaaaaag agcttgagag cggagaatta
    11461 attgaattgc cttttggcga acagtcgcag accattacgg caatgtgcgc acatcatgct
    11521 ggaaaagcgg ttagcccggc gatgcacatt tttattcagt gtgttgaaga gtgttttgtg
    11581 gttgcgtaaa aaaacaatgc cgggcttaaa cccggcattg agattactct tctgtcgttt
    11641 gcggtggcac gcccgttccg ctcggtttac ggcgcttacc aatatttttg gtgtcgcgat
    11701 ggcgtttttt cacccgcggc ttctctttct ctttagcttt tttcttctcg gcgcgttttg
    11761 ccagtacttt cttcgatggc ttgccggtct gcttctcgct tggcgcacgc gttttcgggc
    11821 gtaactcatc aataacgcga gctttaatcg gctcttcaat gtaacggcca accttgccca
    11881 gcagcaaatg gtcatgggct tccaccagcg aaattgcggt gcctttacga ccggcgcgcg
    11941 cggtacgtcc gatacgatgc aaataagtat cgccactgcg cggcatatcg aagttaaaga
    12001 cgtggctgac gtcaggaatg tcgataccgc gcgcggcaac atcggtggca accagtacgt
    12061 ttacgcggcc ttcggtcagg cgtttaattg cttcgttacg cttgccttgt accatctcac
    12121 cttcgagata gcagttgttg atgcccgctt cgcgcagcca gtttgccagc tcatgcacac
    12181 gttcacgctt acgcacaaac acgattgagc gagtcgcttc aggctgtttt aacaggtgca
    12241 ccagcaatgc ggttttatgt tccaggtcat cggcgcggta ataccactga tgaatttttt
    12301 tgcgctcacg ggtggacgga ttggcagaaa cttccaccgg atcttccagc agacgctcgg
    12361 caaagtcctg aatcgcatcg ccttccagcg ttgccgaaaa gagcagggtc tgtttacgcc
    12421 agcgagtttc gccagcaata tgttcgatat cctgagcgaa gcccatatcc agcatacggt
    12481 ctgcttcgtc gaggatcagc gtttcaaccg cgcggcaatc gaagttctct tcttttatgt
    12541 attgcagcag acgtccggtc gtggcgacca cgatgtcctg attttcgctg aacacttccg
    12601 cgtggttcat ataggctacg ccgccggtga tggtggcgat atccagatgc gtatgtttcg
    12661 ccagttcgcg ggcatgatcg gccacctgca tcgccagctc gcgagttggg gtgaggatca
    12721 aaatacgcgg cggaccggat ttcttacgtg ggaaatcgag caggtgctgc aacgctggca
    12781 gcagatacgc cgccgtttta ccggtgcctg tcggcgcaga accgagtaca tcacggccat
    12841 cgagcgcagg cggaatggcg gcagcctgaa tggcggtcgg gcgagtgaaa cctttatcct
    12901 ggagggcttc cagcaggctt tcgtcgagtt caagttcgga aaaagtcgtt acagtcatgt
    12961 tctacctctg tgtggggcgc tgattataga cgttacggct gcaatcttca tctgtttgta
    13021 tggatatcgc ttctcgggta ttgttctcac ccggtgctat cgccgttttt cctgcaaggt
    13081 tttattttca tgtcacagtc tacatccgtg cttcgtcgta atggatttac ttttaaacag
    13141 ttttttgttg ctcacgatcg ctgtgcgatg aaagtgggaa cggatggcat tttgctgggc
    13201 gcatgggcac cggtggctgg ggtgaaacgt tgccttgata tcggcgcggg tagcgggttg
    13261 ctggcactga tgctggcgca gcgaaccagt gacagcgtga taattgatgc cgttgagctg
    13321 gaaagtgaag ctgccgcgca ggcgcaggaa aatatcaacc agtccccgtg ggcagagcgg
    13381 attaacgtcc atacggcgga tattcagcag tggatcacac agcagacagt acgcttcgat
    13441 ttaatcatca gtaacccacc ttactatcag cagggagtag agtgcgcgac acctcaacgg
    13501 gaacaggctc gctataccac tacgcttgat cacccatcgc tgctgacctg tgcggcggag
    13561 tgcattaccg aagagggatt tttctgcgtg gtgctgccag agcagattgg taatggtttt
    13621 actgaactgg cattaagcat gggctggcat ttacgtttgc ggacggatgt ggcggaaaac
    13681 gaagcgcgac tgccgcatcg ggtgctgctg gcattctccc cgcaggcggg agaatgcttt
    13741 agcgatcgct tagtcattcg tgggccagac cagaactatt ccgaagcgta tacggcgctg
    13801 acccaggctt tttatctgtt tatgtaatga ttgccggggg aaaggatcga tggaccggaa
    13861 tgcgtgagca gttccgggta atccagcgta aaatgcagcc cccggctttc tttgcgcatc
    13921 atcgcgcagc gaacaatcaa ctcggcaacc tgtaccagat tacgcagctc cagcaaatta
    13981 tttgagacgc ggaaatgggc gtaatattcg tctatttctt gttggagcat ggttatccgc
    14041 cgcagggcgc gttccaggcg cttcgttgtg cgcacaatgc caacgtaatc ccacataaac
    14101 agccgtagct cgtgccagtt atgctgaatt actacccgtt cgtcagggtt ctcaacgcgg
    14161 ctttcatccc acggcggtaa cgtactgacg ccgtgggcat aaggcatacg tctggtgata
    14221 tcttctgccg ccgaccagcc atagaccaga cactccagca atgaattcga ggccatgcgg
    14281 ttagcgccgt gtaagccggt ataactcacc tcgccaatgg catacaagcc ctcgacgtcc
    14341 gtacgcccat gatcatcaac cattacacca ccgcaggtat agtgtgcagc aggcacaatc
    14401 ggtaccggtt cttgtgtgag atcaatcccc agcccgagca atttttcata aatcatcggg
    14461 aaatgctggc gaataaaatc ggcgggctta tggctgatgt cgaggaacat acaatctgcg
    14521 ccgaggcgtt tcatttcatg gtcaatggcg cgggcgacaa tatcgcgcgg ggccagttcg
    14581 ccgcgctcat caaaatcggg cataaaacgc gtgccatccg ggcgcttgag ataagcgcct
    14641 tcgccgcgca gtgcttccgt taacaggaaa ttgcgtgcct gtgggtgata cagtgcggta
    14701 gggtggaact gattaaattc gagattggca acccgacagc ccgcgcgcca cgccatagca
    14761 atgccatcgc cagaagaaat atccggattg gtggtgtact gatacacctt cgacgcaccg
    14821 ccggttgcca gtaccactgc ttttgcgtgg caggtttcca ccgtttcttt atttcggttc
    14881 catacccacg cgcctacaac ccgtcgcgtg cccggcaggc caattttgtc agagacaatc
    14941 agatcaactg cgttgctgcg ctccagcaca cgaatattcg gatggttctg cgccttgctc
    15001 accagcgtgg attgtacttc tctaccggtg gcgtcggcgg catgaagaat acgacggtga
    15061 ctatgtccac cttcacgggt cagatggtag ctttcttcgc cattcggttg aacgtgggta
    15121 tcaaacaaaa ccccctggtc aattaaccat tgcacacagg aacgagcatt gctggcgaca
    15181 aattcaactg catggcgatc gcaaatacca gccccggaaa tcaatgtgtc ttccacatgc
    15241 gagtcaatgc tgtcagtttc atcaaacacg gcggcaatac cgccctgggc ataaaatgtt
    15301 gaaccttccg ttaccgggcc tttacttaga acgatgacct gatgctggtc agccaggcgt
    15361 agcgccagtg aaagtccggc tgcgccgcta ccaataatca acacgtcaca tgaatgttca
    15421 gggagagtat tcattttctt tgtttaattt actaaacatt gtttggtcag catagcatca
    15481 tgttgtgcga ataaacacct gctattttaa tatttgttac agtggctaaa cacgttgacg
    15541 cagggcggcg agaaaaagag aagttactgg ctggtggagg attaggtggt gaaataaaaa
    15601 ggccgttggg ttactcttca ggcagttaaa tgggcatttc tacacagata atgcgatgtt
    15661 cagattctgt agacttataa tgatagataa tgctccgtct acagcatgac aaacaaaaac
    15721 agatgcgtta cggaacttta caaaaacgag acactctaac cctttgcttg ctcaaattgc
    15781 agctaatgga gtggcgtttc gatagcgcgt ggaaatttgg tttggggaga ctttacctcg
    15841 gatgagcgag cagttaacgg accaggtcct ggttgaacgg gtccagaagg gagatcagaa
    15901 agcctttaac ttactggtag tgcgctatca gcataaagtg gcgagtctgg tttcccgcta
    15961 tgtgccgtcg ggtgatgttc ccgatgtggt acaagaagct tttattaaag cctatcgtgc
    16021 gctggattcg ttccggggag atagcgcttt ttatacatgg ctgtatcgga ttgctgtaaa
    16081 tacagcgaaa aattacctgg ttgctcaggg gcgtcgtcca ccttccagtg atgtggatgc
    16141 cattgaagct gaaaacttcg aaagtggcgg cgcgttgaaa gaaatttcga accctgagaa
    16201 cttaatgttg tcagaagaac tgagacagat agttttccga actattgagt tcctcccgga
    16261 agatttgcgc atggcaataa ccttgcggga gctggatggc ctgagctatg aagagatagc
    16321 cgctatcatg gattgtccgg taggtacggt gcgttcacgt atcttccgag cgagggaagc
    16381 tattgataac aaagttcaac cgcttatcag gcgttgacga tagcgggata ctggataagg
    16441 gtattaggca tgcagaaaga acaactttcc gctttaatgg atggcgaaac gctggatagt
    16501 gagctgctta acgaactggc tcataaccca gaaatgcaga aaacctggga aagctatcac
    16561 ttaatccgtg attcaatgcg gggtgatact cccgaggtgc tccatttcga tatctcttca
    16621 cgcgtgatgg ccgccattga agaagagcca gtacgtcaat cggcaacatt gatcccggaa
    16681 gcccagcctg cgccgcatca atggcagaaa atgccattct ggcagaaagt acgtccgtgg
    16741 gcggcacagc ttacccaaat gggcgtagcc gcatgcgtat cgcttgcagt tatcgttggc
    16801 gtccagcact ataatggaca atctgaaacg tcccagcagc ccgaaacgcc ggtatttaat
    16861 acactgccga tgatgggtaa agccagcccg gtaagcctgg gagtaccttc tgaagcgacc
    16921 gcaaacaatg gtcaacagca gcaggtacag gagcagcgtc gtcgcattaa tgcaatgttg
    16981 caggattacg aactgcaacg ccgactccac tctgaacagc ttcagtttga gcaggcgcaa
    17041 acccagcaag ccgctgtaca ggtgccagga attcaaactt taggaacgca atcgcagtaa
    17101 tgaagcaact ttggtttgcc atgtcattag tgacaggtag cctgttattc tctgctaacg
    17161 cctcggccac tcccgcgtcc ggggcgttat tacagcagat gaacctggcc agtcagtcac
    17221 tgaattacga gctgtcattc atcagcatca ataaacaggg tgttgagtct ctgcgttatc
    17281 gacatgcatg cctcgataac cgtcctcttg cacaattgtt gcaaatggat ggcccgcgcc
    17341 gggaagtggt acagcgcggc aatgaaatca gctattttga accgggactt gaaccgttca
    17401 cgcttaatgg cgattacatt gttgattctc tgccatcgct tatctatact gatttcaaac
    17461 gcctttctcc ttactacgac tttatctccg tcgggcgcac gcgtattgct gatcgtcttt
    17521 gcgaagtcat tcgcgtggtt gcccgagatg gtacacgcta cagctacatc gtgtggatgg
    17581 acaccgaatc gaaattaccg atgcgggttg atctccttga tcgcgatggt gaaacgctgg
    17641 aacaatttcg cgtgattgct tttaacgtca atcaggatat cagcagcagt atgcagacgc
    17701 tggcgaaggc aaatttgccg ccgttgcttt ctgttcctgt aggtgaaaaa gctaaattca
    17761 gctggacgcc aacctggttg ccacagggtt ttagcgaagt ttccagtagt cgacgtccgc
    17821 taccgacgat ggacaacatg cctatcgaat cacgtctcta ttccgacgga ttattcagct
    17881 tctcggtaaa cgttaaccgc gctacgccat cgagcaccga tcagatgttg cgcaccggac
    17941 gcagaaccgt cagtacaagc gtacgtgata acgccgaaat caccattgtc ggtgaactgc
    18001 cgccgcaaac ggcgaaacgc attgccgaga atattaagtt cggggcagcg caatgatcaa
    18061 agagtgggct accgtcgtct cctggcaaaa cgggcaggcg ctggtcagtt gtgatgttaa
    18121 agcttcatgc agcagctgcg cttcacgcgc cggttgcggt agccgcgtgt tgaataaact
    18181 tggcccgcaa accacgcata ccattgtcgt accctgtgat gagccgttag tgccggggca
    18241 aaaagtggaa ttggggatcg ccgaaggcag cctgcttagc tccgcattac tggtttatat
    18301 gtcgccgctg gtgggattat tcctcatcgc ttcgctattt cagctactct ttgcttctga
    18361 cgtcgcagca ttatgtggtg cggtcctcgg cggtattggc gggttcctga ttgcgcgcgg
    18421 ctactcgcgt aagtttgctg cccgggcaga atggcaaccg atcatcttaa gcgtggcatt
    18481 gccgcctggc cttgtgcgat ttgaaacatc ttcggaagac gcgagccagt gatttcactg
    18541 gctttgttgc attttgcata acaaatgcga ggacgtttcc agataccaca atccatacgg
    18601 tatgaacatt tcctcgcctc aatgttgtag tgtagaatgc ggcgtttcta ttaatacaga
    18661 cgttaagctc agaacagcga cttctaaaag cctggttaac cgggcattaa ggcacaataa
    18721 tcatacttta tgaagaatat acgtaacttt tcgatcatag ctcacattga ccacggtaaa
    18781 tcgacgctgt ctgaccgtat tatccagatc tgcggtggcc tgtctgaccg tgaaatggag
    18841 gcgcaggttc tcgattccat ggatcttgag cgtgagcgtg gcattaccat caaagcgcaa
    18901 agcgtgacgc tggactacaa agcgtctgac ggcgaaacct atcagcttaa ctttatcgac
    18961 accccgggcc acgtagactt ctcctatgaa gtttcccgtt cgctggctgc ctgtgaaggt
    19021 gcattgctgg tggtcgacgc cgggcagggc gtagaagcgc aaaccctggc aaactgctac
    19081 accgccatgg aaatggatct cgaagtggtg ccggtactga acaagattga cctgccggca
    19141 gccgatcctg aacgcgtggc ggaagaaatt gaagatatcg tcggcatcga cgccaccgat
    19201 gcagtgcgct gttcagcgaa aaccggcgtt ggcgtgcagg acgttctcga acgtctggtg
    19261 cgcgacattc cgccgccgga aggcgatccg gaaggcccgt tgcaggcact aattatcgac
    19321 tcctggttcg acaactacct gggcgttgtt tcacttatcc gtattaaaaa cggcaccctg
    19381 cgtaagggcg acaaagtgaa agtcatgagt accgggcaga cctataacgc cgaccgtctg
    19441 ggcatcttca cgccgaaaca ggttgaccgc actgaactga aatgtggcga agtaggctgg
    19501 ctcgtatgtg cgattaaaga tatccacggc gctccagtcg gcgatacctt aacgctggcg
    19561 cgtaatccgg cagaaaaggc gctgcctggc tttaagaaag tcaaaccgca ggtatacgcc
    19621 ggtctgttcc cggtaagttc cgacgactat gaagccttcc gtgacgcgct gggtaaactc
    19681 agcctgaacg atgcctcact gttctatgag ccggaaagct ccagcgcgct gggctttggt
    19741 ttccgctgcg gcttccttgg cctgctgcac atggagatca tccaggagcg tctggaacgt
    19801 gaatacgatc tggatctgat caccactgcg ccgaccgtag tgtatgaagt tgaaaccacg
    19861 tcaagggaag ttatctacgt cgacagccca tccaagctgc ctgcggtaaa taacatttac
    19921 gaactgcgcg agccgattgc agagtgtcac atgctgctgc cgcaggcata tctcggcaac
    19981 gttattacgc tgtgcgtaga aaaacgcggc gtgcagacca atatggttta ccacggtaat
    20041 caggtggcgc tgacgtacga gatcccgatg gcggaagtgg tgctcgactt cttcgatcgc
    20101 ctgaaatcta cctcgcgtgg ttatgcgtct ctggattaca acttcaaacg cttccaggcg
    20161 tccgacatgg tacgtgtaga cgtattaatc aacggtgaac gtgttgatgc gctggcgttg
    20221 atcacccacc gtgataattc gcaaaaccgc ggtcgcgagt tggtggagaa gatgaaagat
    20281 ctgatcccac gccagcagtt tgatatcgcc attcaggcag cgattggtac gcacattatt
    20341 gcgcgatcca ccgtgaaaca gctgcgtaaa aacgtactgg ctaaatgtta tggcggcgat
    20401 atcagccgta agaaaaagct gctgcagaag cagaaagaag gtaagaaacg catgaagcag
    20461 atcggtaacg ttgagctgcc gcaggaagcg ttcctcgcca ttctgcacgt cggcaaagac
    20521 aacaaataac ccttaggagt tggcatggcg aatatgtttg ccctgattct ggtgattgcc
    20581 acactggtga cgggcatttt atggtgcgtg gataaattct ttttcgcacc taaacggcgg
    20641 gaacgtcagg cagcggcgca ggcggctgcc ggtgactcac tggataaagc aacgttgaaa
    20701 aaggttgcac cgaagcctgg ctggctggaa accggagctt ctgtttttcc ggtgctggct
    20761 atcgtattga ttgtacgttc gtttatttat gaaccgttcc agatcccgtc aggttcgatg
    20821 atgccgactc tgttaatcgg tgattttatt ctggtagaga agtttgctta tggcattaaa
    20881 gatcctatct accagaaaac gctgatcgaa accggtcatc cgaaacgcgg cgatatcgtg
    20941 gtctttaaat atccggaaga tccaaagctt gattacatca agcgcgcggt gggtttaccg
    21001 ggcgataaag tcacttacga tccggtctca aaagagctga cgattcaacc gggatgcagt
    21061 tccggccagg cgtgtgaaaa cgcgctgccg gtcacctact caaacgtgga accgagcgat
    21121 ttcgttcaga ccttctcacg ccgtaatggt ggggaagcga ccagcggatt ctttgaagtg
    21181 ccgaaaaacg aaaccaaaga aaatggaatt cgtctttccg agcgtaaaga gacactgggt
    21241 gatgtgacgc accgaattct gacagtgccg attgcgcagg accaggtggg gatgtattac
    21301 cagcagccag ggcaacaact ggcaacctgg attgttccgc cgggacaata cttcatgatg
    21361 ggcgacaacc gcgacaacag cgcggacagc cgttactggg gctttgtgcc tgaagcgaat
    21421 ctggtcggtc gggccacggc tatctggatg agcttcgata agcaagaagg cgaatggccg
    21481 actggtgtgc gcttaagtcg cattggcggc atccattaat agccatcttc gttcacgttg
    21541 tcgccgttat ggcgacaacg tgaattattt atgagataaa tctcccgtgg ctaacgacat
    21601 cccccgtcgt tgtgtataga atattccccc gaagtttaag gttggcacct ccaggttgcc
    21661 acggcacacg aaacagcgtt ggtccccata taccgctaaa ctgaaactgc agcgaagcag
    21721 ttagcagagc catgtatatc aggtctgttt cgtgtgctga attgttgacg catttattta
    21781 ttggtatcgc atgaacccca tcgtaattaa tcggcttcaa cggaagctgg gctacacttt
    21841 taatcatcag gaactgttgc agcaggcatt aactcatcgt agtgccagca gcaaacataa
    21901 cgagcgttta gaatttttag gcgactctat tctgagctac gttatcgcca atgcgcttta
    21961 tcaccgtttc cctcgtgtgg atgaaggcga tatgagccgg atgcgcgcca cgctggtccg
    22021 tggcaatacg ctggcggaac tggcgcgcga atttgagtta ggcgagtgct tacgtttagg
    22081 gccaggtgaa cttaaaagcg gtggatttcg tcgtgagtca attctcgccg acaccgtcga
    22141 agcattaatt ggtggcgtat tcctcgacag tgatattcaa accgtcgaga aattaatcct
    22201 taactggtat caaactcgtc tggacgaaat tagcccaggc gataaacaaa aagatccgaa
    22261 aacgcgcttg caagaatatt tgcagggtcg ccatctgccg ctgccgactt atctggtagt
    22321 ccaggtacgt ggcgaagcgc acgatcagga atttactatc cactgccagg tcagcggcct
    22381 gagtgaaccg gtggttggca caggttcaag ccgtcgtaag gctgagcagg ctgccgccga
    22441 acaggcgttg aaaaaactgg agctggaatg agcatcgata aaagttactg cggatttatt
    22501 gccatcgtcg gacgtccgaa cgttggcaaa tccacattgt tgaacaaact gctggggcag
    22561 aaaatctcca tcacctcccg caaggcgcag acaactcgtc accgcattgt ggggatccat
    22621 actgaaggcg cgtatcaggc gatctacgtc gatacaccgg gcctgcatat ggaagaaaaa
    22681 cgcgccatta accgcctgat gaacaaagcg gcgagcagct ctattggcga tgttgagctg
    22741 gtgatttttg tcgttgaagg cacccgctgg acgccggacg acgaaatggt gctcaacaaa
    22801 ctgcgcgaag gcaaagcgcc ggtaatcctc gcggtgaaca aagtggacaa cgtgcaggag
    22861 aaagccgatc tgctgccgca cctacagttc ctggcaagcc agatgaactt cctcgatatc
    22921 gtgccaatct ctgccgaaac cgggctgaat gttgacacta ttgcggcaat cgtgcgtaag
    22981 catctgcctg aagcgactca tcacttcccg gaagattaca tcaccgatcg ctcacagcgt
    23041 tttatggcgt ctgaaatcat ccgcgaaaaa ctgatgcgtt tccttggcgc tgaactgccg
    23101 tactccgtga ccgtggagat cgaacgtttc gtctctaacg aacgcggcgg ttatgacatc
    23161 aacggtttga ttctcgttga gcgtgaaggg cagaagaaga tggttattgg caacaaaggg
    23221 gccaagatca aaaccatcgg gattgaagcg cgtaaagaca tacaggaaat gttcgaagcg
    23281 cctgtccacc ttgaactgtg ggtaaaagtg aaatcgggtt gggccgacga cgaacgcgca
    23341 ctgcgcagtc tcggttatgt tgacgatctt taagagtcac tccgatggaa ggctggcagc
    23401 gcgcatttgt cctgcatagt cgcccgtgga gcgaaaccag cctgatgctg gacgtcttca
    23461 cagaggaatc gggtcgcgtg cgtctggttg ccaaaggcgc acgctctaaa cgctctactc
    23521 tgaaaggtgc attacagcct ttcacccctc tcttgctacg ttttggcggg cgtggcgaag
    23581 tcaaaacgct gcgcagtgct gaagctgtct cactggcgct gccattaagc ggtatcacgc
    23641 tttacagcgg tctgtacatc aacgaacttc tctcccgcgt actggaatac gagacgcgct
    23701 tctctgaact ctttttcgat tacttgcact gcattcagtc tcttgcaggt gccactggta
    23761 cgccagaacc cgcgctgcgt cgctttgaac tggcactact cgggcatctg ggctatggcg
    23821 tcaattttac ccattgcgcg ggtagcggcg agccggtaga tgacaccatg acgtatcgtt
    23881 atcgcgaaga aaaagggttt atcgcaagcg tcgttatcga caataaaacg ttcactggaa
    23941 ggcagttaaa agcgttaaac gcgcgggaat ttcctgacgc agacacacta cgcgccgcga
    24001 aacgctttac ccgaatggcg cttaagccgt atcttggcgg taaaccatta aagagcaggg
    24061 aactgttccg gcagtttatg cctaagcgaa cggtgaaaac acattatgaa tgatgaggat
    24121 tgtcatggct gaattactgt taggcgtcaa cattgaccat attgcaacgc tgcgtaacgc
    24181 acgcggtact gcttacccgg atccggtgca ggcggcgttt attgccgagc aggcgggagc
    24241 ggacggcatt accgtgcatt tacgtgaaga tcgccgccac attactgacc gcgatgtgcg
    24301 catcctgcgg cagacgctgg atacccgaat gaatctggag atggcagtga ccgaagagat
    24361 gctggcgatc gccgttgaga cgaagccaca tttttgctgc ttggtaccgg aaaagcgtca
    24421 ggaagtaaca accgaaggcg gcctggacgt tgcagggcag cgtgaaaaaa tacgcgatgc
    24481 ctgcaaacgt ctggcagatg ccgggattca ggtttctctg tttatcgacg ccgatgaaga
    24541 gcagatcaaa gctgcggcag aggttggcgc accgtttatc gagatccaca ccggttgcta
    24601 tgctgatgcc aaaactgacg ccgaacaggc gcaagagctg gtgcgtatcg ccaaagccgc
    24661 gacctttgcc gctagtctcg gtctgaaagt taacgccgga cacggtctga cctatcacaa
    24721 cgtgaaagcc attgccgcca tccctgagat gcatgaactg aatatcggtc atgccattat
    24781 tggccgtgca gtgatgaccg gactgaaaga tgcggtggca gaaatgaagc gtctgatgct
    24841 ggaagcgcgt ggctaatggc aatattaggt ttaggcacgg atatcgtaga gatcgctcgc
    24901 atagaagcag tgatcgcccg atccggtgag cgcctggcac gccgcgtatt aagcgataac
    24961 gaatgggcaa tctggaaaac gcaccaccag ccggtgcgtt ttctggcgaa gcgttttgct
    25021 gtgaaagaag ccgcagcaaa agcgtttggc accgggatcc gcaatggtct ggcgtttaat
    25081 caatttgaag tattcaatga tgagctcggc aaaccacggc tacgactatg gggtgaggca
    25141 ttaaaactgg cggaaaagct cggcgttgta aatatgcatg taacgctggc agatgaacgc
    25201 cactatgctt gtgccacggt aattattgaa agttaaaata ataccgttct ctttaaaaag
    25261 taaaaccccc gaactggtaa ggaacggggg ttttactttt taactttaat tttgtgcagc
    25321 atgcaccctc aacaacagcg attatggatc agtgtactta tcttttacaa gcaacttgca
    25381 atctttagca taaaaactcg agcctttacg aagaaagcaa tattgatgga aagattaacg
    25441 tgaccgccaa ttcgtaagta cattaaaatt ggcttcgtta ttgaagattt tgctgtgctt
    25501 tacaccatgc cacagaattc ccccattgaa acgagtggtg tcgtcaaagc tctggtgtgg
    25561 agtgcagcat gcaccctcaa taactcgcac gttcagtttt ggggagatgt aagggctaat
    25621 ctgaatggct gcattccttg tttaaggaaa aacgaatgac tgattgccga tacctgatta
    25681 aacgggtcat caaaatcatc attgctgttt tacagctgat ccttctgttc ttataacaca
    25741 aggaaacgta cttaaggtgc gtccggtgaa ccaatcggac gcgcctttaa taactataaa
    25801 taagtgtctt ggcagatact atataaatta acttagtgaa tgattatgct aatgtcatca
    25861 attaaataaa tataatggcg ttaaggcttc ccagtaatat aattaatact ctacttccag
    25921 agtagaatat taaattttat ccgcgtggtg catcagcaca aatttatccc acaactgttc
    25981 ttctgtctcg acatgcgccg gatctttcac aatagtattg gggatcgggc acaccttctg
    26041 gcaggttggt gtctcgtagt gccctacgca ttcggtacac ttatcgctgt taatttcgta
    26101 gatatgatct cccattgaaa tcgcctcatt cgggcattcg ggttcacaca tatcacaatt
    26161 aatacagcgt ttagtaatta gtaaagacat ttcaatggat taccattaaa tcattttaaa
    26221 atcagtaagt tgtatcgagt ttgtatgctt tactgtcatt aacttactgt atgttgatcc
    26281 agtgtatttc accttgataa actcagtcca gcaacacaaa accgcaacac attgcatttt
    26341 gtcccgtaga aaagacttgt atgtgtgagc ttgttttctg cgcctacgca gataaggatt
    26401 gagaatgccg cgcactgtaa cacataatcc ggatagcccc aataatgacg atgttttagc
    26461 cgcatctgaa aaatgggacg cctgtaaacc cccctatacc agcgcacaca tgaaaatctg
    26521 tgttgctgcc gccaaaatca tcctcgctgc ttccggcgtg gctcgccgtt ccaaatacga
    26581 aaaagagaac tatctccgta tcgatttcag caaagccggt aaggttacat tttacgccga
    26641 gtttccaaaa aagatgggcc tcaaaggtaa aaagctcggc gagtggccgg agctcgctat
    26701 ccagctggcg cgcgaaaaag cactaggtat ggctgacggt ggactgcggg cagagtccgt
    26761 acatgcagcg ctggaaatgt accgggatga cctcaaagcc aaagtagccc ggcagaagct
    26821 gagcccggac agtttcacaa cctacggggt gcgtatcgac cggattaaag caacgttcgg
    26881 cgagcgtgag gtgttcagcg acgtaacata caatcggctg gtggaagtgc tggacgagtg
    26941 gatcgccact cgctcgaaca ataacgccct ggagttgttt gccgagctcc gtcggttctg
    27001 gaagttctgc gcacctactc tttgcaacgg ccgcaatgtt gccgccagtc tgccagatga
    27061 ttatgtttcc ttccgcgtac agaaacctac ccccacacgg ctttttaccg atattgaatc
    27121 aatcgcccga ctctggctca atgttgct
//
LOCUS       DAJTYG010000049        27127 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTYG010000049 DAJTYG010000000
VERSION     DAJTYG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27127
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.049"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..437
                     /locus_tag="OO568_002234"
     CDS             <1..437
                     /locus_tag="OO568_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCR6641575.1"
                     /translation="IIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQV
                     ELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVETLAWF
                     TVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="OO568_002235"
     CDS             complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="OO568_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6641576.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            1230..2252
                     /gene="pldB"
                     /locus_tag="OO568_002236"
     CDS             1230..2252
                     /gene="pldB"
                     /locus_tag="OO568_002236"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6641577.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWHQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            2260..3060
                     /gene="yigL"
                     /locus_tag="OO568_002237"
     CDS             2260..3060
                     /gene="yigL"
                     /locus_tag="OO568_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6641578.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            3136..4035
                     /gene="bioP"
                     /locus_tag="OO568_002238"
     CDS             3136..4035
                     /gene="bioP"
                     /locus_tag="OO568_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6641579.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(3923..4876)
                     /gene="metR"
                     /locus_tag="OO568_002239"
     CDS             complement(3923..4876)
                     /gene="metR"
                     /locus_tag="OO568_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6641580.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            5112..7373
                     /gene="metE"
                     /locus_tag="OO568_002240"
     CDS             5112..7373
                     /gene="metE"
                     /locus_tag="OO568_002240"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6641581.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(7412..8227)
                     /locus_tag="OO568_002241"
     CDS             complement(7412..8227)
                     /locus_tag="OO568_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6641582.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            8489..9250
                     /gene="udp"
                     /locus_tag="OO568_002242"
     CDS             8489..9250
                     /gene="udp"
                     /locus_tag="OO568_002242"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6641583.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            9391..10818
                     /gene="rmuC"
                     /locus_tag="OO568_002243"
     CDS             9391..10818
                     /gene="rmuC"
                     /locus_tag="OO568_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6641584.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPGQPNDEAYQRDDEYNQQSR"
     gene            10913..11668
                     /gene="ubiE"
                     /locus_tag="OO568_002244"
     CDS             10913..11668
                     /gene="ubiE"
                     /locus_tag="OO568_002244"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6641585.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            11682..12287
                     /gene="ubiJ"
                     /locus_tag="OO568_002245"
     CDS             11682..12287
                     /gene="ubiJ"
                     /locus_tag="OO568_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6641586.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            12284..13924
                     /gene="ubiB"
                     /locus_tag="OO568_002246"
     CDS             12284..13924
                     /gene="ubiB"
                     /locus_tag="OO568_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6641587.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            14003..14272
                     /gene="tatA"
                     /locus_tag="OO568_002247"
     CDS             14003..14272
                     /gene="tatA"
                     /locus_tag="OO568_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6641588.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            14276..14791
                     /gene="tatB"
                     /locus_tag="OO568_002248"
     CDS             14276..14791
                     /gene="tatB"
                     /locus_tag="OO568_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6641589.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            14794..15570
                     /gene="tatC"
                     /locus_tag="OO568_002249"
     CDS             14794..15570
                     /gene="tatC"
                     /locus_tag="OO568_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6641590.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            15612..16394
                     /gene="tatD"
                     /locus_tag="OO568_002250"
     CDS             15612..16394
                     /gene="tatD"
                     /locus_tag="OO568_002250"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6641591.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="OO568_002251"
     CDS             complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="OO568_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6641592.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            17046..18539
                     /gene="ubiD"
                     /locus_tag="OO568_002252"
     CDS             17046..18539
                     /gene="ubiD"
                     /locus_tag="OO568_002252"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6641593.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVTYIDAIWDELAIFNNGKSA"
     gene            18585..19286
                     /gene="fre"
                     /locus_tag="OO568_002253"
     CDS             18585..19286
                     /gene="fre"
                     /locus_tag="OO568_002253"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6641594.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="OO568_002254"
     CDS             complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="OO568_002254"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6641595.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARIHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="OO568_002255"
     CDS             complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="OO568_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6641596.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEITAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            22931..24262
                     /gene="pepQ"
                     /locus_tag="OO568_002256"
     CDS             22931..24262
                     /gene="pepQ"
                     /locus_tag="OO568_002256"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6641597.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVITLPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLGLIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            24262..24876
                     /locus_tag="OO568_002257"
     CDS             24262..24876
                     /locus_tag="OO568_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6641598.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            24915..26366
                     /gene="trkH"
                     /locus_tag="OO568_002258"
     CDS             24915..26366
                     /gene="trkH"
                     /locus_tag="OO568_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6641599.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPAINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            26378..26923
                     /gene="hemG"
                     /locus_tag="OO568_002259"
     CDS             26378..26923
                     /gene="hemG"
                     /locus_tag="OO568_002259"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6641600.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHAMRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6229 a         6905 c         7473 g         6520 t
ORIGIN      
        1 tgattatcga aaaaatggcc tggctgcata cgctgattat ggtgggcggt ggcctgtatc
       61 tctgctggat gggttaccag atgctacgcg gtgcactgaa aaaagaggct gtatctgcgc
      121 ctgcaccaca ggttgagctg gcgaaaagcg ggcgcagttt cctgaaaggc ttactgacca
      181 atctcgccaa tccgaaagcg attatctatt ttggttcggt attctcattg tttgtcggcg
      241 ataacgttgg cactaccgag cgctggggca ttttcgcgtt gattattgtc gaaacactgg
      301 cgtggtttac cgtcgttgcc agtctgtttg ccctgccgca aatgcgccgt ggttatcaac
      361 gtctggcgaa gtggattgat ggttttgctg gggcgttatt tgccggattt ggcattcatt
      421 tgattatttc gcggtgatgc cagacgcgtc ttcagagtaa gtcggataag gcgtttacgc
      481 cgcatccgac attatttttc acgcatgcct cgccgatgct aacagcgctc ccaccagcat
      541 aaacaacgag ccgaaaatct tattcagcgc cttcatctgc tttggtcctt taatccatag
      601 agcaatcctt tgagcaaggg tggcgtaacc gatcatcaca ataatatcga ccacaatagt
      661 ggtgacgccg agcacgatat actgcatcag ttgcggctgt tgcggcatga tgaattgcgg
      721 aaatagcgcc gccagaaaca caatactttt gggattggtg agattcacaa aaactgcgcg
      781 ctggaacaaa tgtcgacgcg attgagtaga ggccagcgat ttaagatcaa ttgcgccagc
      841 ggcgcgccac tgctggattc ccagccaaat caagtaagcc gcgcctgccc actttaacac
      901 ttcaaacgca atcactgagc gggaaaatag cgtccccaac cccacgccaa ccagcacaat
      961 atgaatcgcc agtccggtct gaagcccagc aatagacgcc accgcgccgc gataaccgtg
     1021 gttgagcgag gtggtcatag tgttgattgc accagagcct ggcgacaggc ttaaaatgat
     1081 cgatgtcagc aggtaggcaa accaccattc taaggtcatg atgaactccc ggtgtgtcta
     1141 tttttgtgcc acaatacgct actgtcgcag cgttgtgtca ggcacgctta aaaaaacgat
     1201 tttacgtggt ttaagaggca gattacccga tgtttcagca gcaaaaagac tgggaaacaa
     1261 gagaaaacgc gtttgctgct tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg
     1321 atgaagcaga gtttactggt gtggatgaca ttccggtgcg ctttgtccgt tttcgcgcac
     1381 agcaccatga ccgggtggta gtcatctgcc cggggcgtat tgagagctac gtaaaatatg
     1441 cggaactggc ctacgacctg ttccatttgg ggtttgatgt cttaatcatc gaccatcgcg
     1501 ggcagggacg ttccggtcgc ctgttagccg atccgcatct cgggcatgtt aatcgcttta
     1561 atgattatgt tgatgatctg gcggcattct ggcatcagga ggttcagccc ggtccgtggc
     1621 gtaaacgcta tatactggca cattcgatgg gcggtgcgat ctccacatta tttctgcaac
     1681 gccatccagg tgtatgtgac gccattgcgc taactgcgcc aatgtttggg atcgtgattc
     1741 gtatgccgtc atttatggca cggcagatcc tcaactgggc cgaagcgcat ccacgtttcc
     1801 gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac
     1861 tgacccacag cagacagcga tatcgacgta acttacgctt ctatgctgat gacccaacga
     1921 ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag tattctggct ggcgaacagg
     1981 tgttagccgg tgcgggtgat gacgccacgc caacgcttct cttacaagct gaagaggaac
     2041 gcgtggtgga taaccgcatg cacgaccgtt tttgtgaact ccgcaccgcc gcgggccatc
     2101 ctgtcgaagg aggacggccg ttggtaataa aaggtgctta ccatgagatc ctttttgaaa
     2161 aggacgcaat gcgctcagtc gcgctccacg ccatcgttga ttttttcaac aggcataact
     2221 cacccagcgg aaaccgctct acagaggttt aaatttctta tgtaccaggt tgttgcgtct
     2281 gatttagatg gcacgttact ttctcccgac catacgttat ccccttacgc caaagaaact
     2341 ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt
     2401 gatgtggggc aaattcgcga taatctggag attaagtctt acatgattac ctccaatggt
     2461 gcgcgcgttc acgatctgga tggcaatctg atttttgctc ataacctgga tcgcgacatc
     2521 gccagcgatc tgtttggcgt agtcaacgac aatccggaca tcattactaa cgtttatcgc
     2581 gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga tgcgcttttt taaagaagcg
     2641 gtgttcaaat atgcgctgta tgagccggga ttactggagc cggaaggcgt cagcaaagtg
     2701 tttttcacct gcgattccca tgaacaactg ctgccgctgg agcaggcgat taacgctcgc
     2761 tggggcgatc gcgtcaacgt cagtttctct accttaacct gtctggaagt gatggcgggc
     2821 ggcgtgtcga aaggccatgc gctggaagca gtggcgaaga aactgggcta cagcctgaag
     2881 gattgtattg cgtttggtga cgggatgaac gacgccgaaa tgctgtcgat ggcggggaaa
     2941 ggctgcatta tgggcagtgc gcaccagcgt ctgaaagatc tgcatccgga gctggaagtt
     3001 attggtacta atgccgacga cgcggtgccg cattatctgc gtaaactcta tttatcgtaa
     3061 tcgttcttta tttggtcagt tgtcaacctg atacttcgct acaatggata ctcgttaatc
     3121 aaagagtttt ccattgtggc gctacttatc atcaccacga ttctgtgggc cttctccttt
     3181 agcttttatg gcgagtacct tgcggggcac gtcgatagct attttgcggt gctggtgcgc
     3241 gttggcttgg cggcactcgt ttttctgccg tttctgcgta cccgtagcaa tagcctgaaa
     3301 acggtcggcc tgtatatgct ggtgggcgcg atgcagctcg gcgtgatgta tatgctgagt
     3361 ttccgcgctt atctctacct gacggtctcc gaattgttgc tgttcaccgt actgacgccg
     3421 ctctacatca cgctgattta tgacatcatg agtaagcgcc gtctgcgctg gggctatgct
     3481 tttagcgcct tgctggcggt gattggtgcc gggattattc gctatgatca ggtcaccgac
     3541 catttctgga ctggcttgtt gctggtgcaa ctctccaata tcacttttgc catcggcatg
     3601 gtgggttaca aacgcctgat ggaaactcgc ccgatgccgc agcataacgc ctttgcgtgg
     3661 ttctatcttg gcgcgttcct ggtggcggta attgcatggt tcttgctggg aaatgcgcag
     3721 aaaatgccgc aaaccacgtt gcaatggggc attctggtgt ttcttggcgt ggtggcttcc
     3781 gggattggct actttatgtg gaactacggc gcgacgcagg tggacgccgg aacgctgggc
     3841 attatgaata atatgcacgt tccggcaggg ctgctggtaa acctggctat ctggcaccaa
     3901 cagccgcact ggccaacgtt tattacaggc gcgctggtga tcctggcctc actgtgggtg
     3961 catcgtaagt gggtcgctcc gcgctcttca caaacggcag atgatcgcag gcgtgattgc
     4021 gcgctgagcg aataaacgct tccgtaactg gctgacgctg ctcgccatcg cgcacggcgg
     4081 cgtacagtcg gctccacaag ccttcgccca gggttttggt caccaccaga ccctggcgct
     4141 caaaactctc tactacccaa tgcggtagcg cggcaatacc catccgcgcg gcaaccattt
     4201 gaatcaataa taaggtgtta tcgacgcttt tcagtgacgg gctgacgcct gccggctgaa
     4261 gaaaatgccg ccagacatcc agtcgactac gctgcaccgg ataaattaat agcgtctcgc
     4321 tggcgagatc ttccggtgta atacgcgttt ttgccgccag tggatggtca ggtgctaaca
     4381 ccaggcgtac ttcataatcg aacatcggcg aataatgcag gccactgcgc ggcaggatat
     4441 cggaggtcat caccagatcc agctcgccct gttgcaacgc gggctgcggg tcaaacgtca
     4501 cgcccgattt aaaatccatc tctacctgcg gccagttctt atggaaattt tctaaagcgg
     4561 gtgtcagcca ctgaatacag ctatggcact caatggcaat gcgcagacgc gtctgctgcg
     4621 gttcattgca ggcttgcagt gcctggctga tttgcggcag cacctggttt gccagttgca
     4681 acaggatttc tccctgcggt gtaaagcgta gcggctggct cttacgcaca aatagccgga
     4741 agccaaggcg ttgttccaga tcgctaaact ggtgagacag ggcggattgc gtctgatgca
     4801 acgttgccgc agcggctgcg agcgagccgc agttccgcaa cgcttgtagc gttttcaggt
     4861 gttttacttc gatcatgaaa gtccttcact tcggcatgaa taatttgcgc ttgaggaata
     4921 tacagtaacc gccaattatg gatgtgtaaa catctggacg gctaaaatcc ttcgtctttt
     4981 aaatttatgg tgcgttggct gcgtttctcc accccggtca cttacttcag taagctcccg
     5041 gcgatgaata aacttgccgc cttccctaaa ttcaaaatcc ataggattta catataatta
     5101 gaggaagaaa aatgacaata ttgaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc
     5161 gcgagctgaa aaaagtgcaa gaaagttatt gggcggggaa ctccacgcgt gaagaactgc
     5221 tgacagtagg gcgtgaactg cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg
     5281 acctgctgcc ggtgggcgat tttgcctggt acgatcatgt actgaccacc agtttgctgc
     5341 tgggtaatgt tccgccacgt catcagaaca aagacggttc ggtagatatc gacaccctgt
     5401 tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca
     5461 aatggtttaa caccaactat cactacatgg tgccggagtt cgttaaaggc caacagttca
     5521 aactgacctg gacgcagctg ctggaggaag tggacgaggc gctggcgctg agccacaacg
     5581 ttaagcctgt gctgctgggg ccggttactt acctgtggct ggggaaagtg aaaggtgaac
     5641 agtttgaccg ccttagcctg ctgaacgaca ttctgccggt ttatcagcaa gtgctggcag
     5701 aactggcgaa acgcggcatc gagtgggtac agattgatga acccgcgctg gtactggaac
     5761 taccacaggc gtggctggac gcatacaaac ccgcttacga cgcgctccag ggacaggtga
     5821 aactgctgct gaccacctat tttgaaggcg taacgccaaa tctcgacacg attactgcgc
     5881 tgcctgttca ggggctgcat gtggaccttg tacatggtaa agatgacgtt gttgaactgc
     5941 acaagcgcct gccttctgac tggctgctgt ctgcgggtct gatcaatggt cgtaacgtct
     6001 ggcgcgccga tcttactgag aaatatgcgc aaattaagga cattgtcggc aaacgtgatt
     6061 tgtgggtggc atcttcctgc tcgttgctgc acagccccat cgacctgagc gtggaaacgc
     6121 gtcttgatgc agaagtgaaa agctggtttg ccttcgccct acaaaaatgc catgaactgg
     6181 cactgctgcg cgatgcgctg aacagtggtg acacggcagc tctggcagag tggagcgccc
     6241 caattcaggc gcgtcgtcac tctactcgtg tacataatcc ggcggtagaa aagcgtctgg
     6301 cggcgatcac cgcccaggac agccagcgtg cgaatgtcta tgaagtgcgt gctgaagccc
     6361 agcgtgcgcg ttttaaactg ccagcgtggc cgaccaccac gattggttcc ttcccgcaaa
     6421 ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg caatctcgac gccaacaact
     6481 accgcacggg cattgcggaa catatcaagc aggccattgt tgagcaggaa cgtttgggac
     6541 tggatgtgct ggtacatggc gaggccgagc gtaatgacat ggtggaatac tttggcgagc
     6601 acctcgacgg atttgtcttt acgcaaaacg gttgggtaca gagctacggt tcccgctgcg
     6661 tgaagccacc gattgtcatt ggtgacatta gccgcccggc accgattacc gtggagtggg
     6721 cgaagtatgc gcaatcgctg accgacaaac cggtgaaagg gatgctgacg gggccggtga
     6781 ccatactctg ctggtcgttc ccgcgtgaag atgtcagccg tgaaaccatc gccaaacaga
     6841 ttgcgctggc gctgcgtgat gaagtggccg atctggaagc cgctggaatt ggcatcatcc
     6901 agattgacga accggcgctg cgcgaaggtt taccgctgcg tcgtagcgac tgggatgcgt
     6961 atctccagtg gggcgtagag gccttccgta tcaacgccgc cgtggcgaaa gatgacacac
     7021 aaatccacac tcacatgtgt tattgcgagt tcaacgacat catggattcg attgcggcgc
     7081 tggacgcaga cgtcatcacc atcgaaacct cgcgttccga catggagttg ctggagtcgt
     7141 ttgaagagtt tgattatcca aatgaaatcg gtcctggcgt ctatgacatt cactcgccaa
     7201 acgtaccgag cgtggaatgg attgaagcct tgctgaagaa agcggcaaaa cgcattccgg
     7261 cagagcgcct gtgggtcaac ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc
     7321 gcgcggcact ggcgaacatg gtgcaggcgg cgcagaattt gcgtcggggg taaatccaaa
     7381 ccgggtggta ataccacccg gtcttttctc attacagcga cttcttccca ccatactgct
     7441 taaaccattc cagcatacgc tgccagccat ctttcgcaga ttcggcatga tagctcgggc
     7501 gataatcagc gttgaatgca tgcccggcat ccgggtacac gataatctct gctttcgcat
     7561 tagcagcccg cagcgcctgg cgcatcgttt caacgctctc ctgcggaatg ctgttatcct
     7621 gaccaccata taagccgaga accggcgcgt taagatcggt tgcgatatca acaggttgtt
     7681 tcggtgaatt cagcgacttg tcgcccgtca gtttgccgta ccacgccact gcggctttta
     7741 gctgtggatt atgcgcggca tacagccagg tgatacgtcc accccagcag aatccggtga
     7801 tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcgaca tgatcgagat
     7861 cggccagcac ctgcgagtca ggcacttttg ctaccagacc gctaagcaac gtggggatat
     7921 cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcgata gccagatacc
     7981 cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca aaaatttcct
     8041 gcactacaat gaccactggc agtgggccat cgctttgctt tggtctggca tggtaagcag
     8101 gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg tcgtccgggg
     8161 tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgc aaatccagat tgttgtgttg
     8221 ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact atagtcagca
     8281 tgcaacaaat cacattgcct gaatcggctc atcttttatg cagtcctgca gaatgaagag
     8341 tgatttatgt gatatgaatc acttttggtg ggtaaattta tgcaacgcat ttgcgtcatg
     8401 gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg
     8461 actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc atctcggcct
     8521 cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc cggatcgtgt
     8581 ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc gcgaattcac
     8641 tacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgctctaccg gtatcggcgg
     8701 cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca ccttcctgcg
     8761 tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc tggttaccac
     8821 ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca ccgctggaat tcccggctgt
     8881 cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg aaatctattg gcgcgacaac
     8941 tcacgttggc gtgacagttt cttctgatac cttctaccca ggtcaggaac gttacgatac
     9001 ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc aggcgatggg
     9061 cgtaatgaac tatgaaatgg aatctgcaac tctgctgacc atgtgtgcaa gccagggcct
     9121 gcgtgccggt atggtagcgg gtgttatcgt taaccgcacc cagcaagaga tcccgaatgc
     9181 tgagacgatg aaacaaactg aaagccatgc ggtgaaaatc gtggtggaag cggcgcgtcg
     9241 tctgctgtaa ttctcttctc ctgtctgaag gccgacgcgt tcggcctttt gtatttttgc
     9301 gtagcgcctc gcaggaaatg cctttccaac tggacgtttg tacagcacaa ttctattttg
     9361 tgcgggtaag ttgttgcgtc aggaggcgtt gtggatttct caatcatggt ttacgcagtt
     9421 attgcgttgg tgggtgtggc aattggctgg ctgtttgcca gttatcaaca tgcgcagcaa
     9481 aaagccgagc aattagctga acgtgaagag atggtcgcgg agttaagcgc ggcaaaacaa
     9541 caaattaccc aaagcgagca ctggcgtgca gagtgtgaat tgctcaataa cgaagtgcgc
     9601 agcctgcaaa gtattaacac ctctctggag gccgatctgc gtgaagtaac cacgcggatg
     9661 gaagccgcac agcaacatgc tgacgataaa attcgccaga tgattaacag cgagcagcgc
     9721 ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg agcacagcaa tcgccgggtt
     9781 gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc cgctacgtga acaactggac
     9841 ggtttccgcc gtcaggttca ggacagcttc ggtaaagaag cacaagaacg ccataccctg
     9901 acccacgaaa ttcgcaatct ccagcaactc aacgcgcaaa tggcccagga agcgatcaac
     9961 ctgacgcgcg cgctgaaagg cgacaataaa acccagggca actggggcga ggtagtattg
    10021 acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg aatatgaaac ccaggtcagc
    10081 atcgaaaatg acgcccgctc gcggatgcag ccggatgtca tcgtgcggct gccgcaggga
    10141 aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta ttttaacgcc
    10201 gaagacgact acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat
    10261 atccgtttgc tgggacgcaa agattatcaa cagctgccgg ggctgcgaac tctggattac
    10321 gtgctgatgt ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag
    10381 ctgatcaccg aagcgttgaa aaacaatatc atgctggtta gcccgactac gctgctggtg
    10441 gcgttgcgta ctattgccaa cctgtggcgt tatgagcatc aaagccgtaa cgcccagcaa
    10501 atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga tgacatgtcc
    10561 gcgattggtc aaagtctcga caaagcgcag gataactacc gccaggcaat gaaaaaactc
    10621 tcttcaggtc gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggagtagaa
    10681 attaaacgcg agattaatcc ggatttggct gaacaggcgg tgagccagga tgaagagtat
    10741 cgacttcggt cggttccggg gcagccgaat gatgaagctt atcaacgcga tgatgaatat
    10801 aatcagcagt cgcgctagcc cattaggggt agttaagccg ggtagaaatc tagggcatcg
    10861 acgcccaatc tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga
    10921 taagtcacaa gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga
    10981 tatggtcgcc cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat
    11041 gtcatttggt attcatcgtt tgtggaagcg attcacgatt gattgcagcg gcgtacgccg
    11101 tgggcagacc gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg
    11161 cctggtcgga gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat
    11221 gggacgcgag aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa
    11281 cgctgaggcg ctgccgttcc cggataacac ctttgattgc atcaccattt cgtttggtct
    11341 gcgtaacgtc accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg
    11401 cggccgcctg ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta
    11461 tgatgcatac tccttccatg tgctgccgcg tattggctca ctggtcgcga acgacgccga
    11521 cagctaccgt tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc
    11581 catgatgcag gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt
    11641 ggcgctgcat cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag
    11701 tgacggcagg aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc gcgctgaaaa
    11761 cagcccgctc gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc ttttcgacgt
    11821 cattgattct ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg
    11881 ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat cgccagcagc
    11941 ttaccgcact gattcgcagt ggtgagctgg aagtgcaggg cgatattcag gtggtgcaaa
    12001 acttcgttgc gctggcagat ctggcagagt tcgaccctgc ggaactgctg gccccttata
    12061 ccggtgatat cgccgctgaa ggaatcagca aagccatgcg cggaggcgca aagttcctgc
    12121 atcacggcat taagcgccag caacgttatg tggcggaagc cattactgaa gagtggcgta
    12181 tggcacccgg tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg
    12241 ctgttgatgc cctgaccaaa cggctggaaa aactggaggc taaatgacgc ctggtgaagt
    12301 acggcgccta tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc
    12361 caaaatgcgt atcaccctgc cgctacggct atggcgatac tcattattct ggatgccaaa
    12421 tcggcataaa gacaaacttt taggtgagcg actacgactg gccctgcaag aactggggcc
    12481 ggtttggatc aagttcgggc aaatgttatc aacccgccgc gatctttttc caccgcatat
    12541 tgccgatcag ctggcgttat tgcaggacaa agttgctccg tttgatggca agctggcgaa
    12601 gcagcagatt gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga
    12661 aatcaagccg ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa
    12721 tggtaaagag gtggtgatta aagtcatccg cccggatatt ttgccggtca ttaaagcgga
    12781 tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg
    12841 tctgcgccca accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg aactgaattt
    12901 gctgcgggaa tctgccaacg ccattcagct tcggcgcaat tttgaagaca gcccgatgct
    12961 ctacatcccg gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat
    13021 ttacggcatt ccggtgtctg atgttgcggc gctggagaaa aacggcacta acatgaaatt
    13081 gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca
    13141 tgccgatatg caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata
    13201 tatcggcatt gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc
    13261 agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga
    13321 ttctggctgg gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt
    13381 ctgtgaacct atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa
    13441 tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca
    13501 gaaaaccctg ctctatgtcg aaggggtagg acgccagctt tatccgcagc tcgatttatg
    13561 gaaaacagcg aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgccct
    13621 ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga
    13681 actggtttac gacagtttgc ggcagggcaa gtatttacag cacagtgttg ataagattgc
    13741 ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg
    13801 cgctacgtta gtattaagtg gcacattctt gttggtcagc cgacctgagt gggggctgat
    13861 gcccggctgg ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg
    13921 ctgatttttt catcgctcaa ggcgggccgt gtaacgtata atgcggcttt gtttaatcat
    13981 catctaccac agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc
    14041 tgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg
    14101 tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac
    14161 cagtcaggat gctgatttta ctgcgaaaac tatcgccgat aagcaggcgg atacgaatca
    14221 ggaacaggct aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt
    14281 tgatatcggt tttagcgaac tgctattggt gttcatcatc ggcctcgtcg ttctggggcc
    14341 gcaacgactg cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt tgcgttcact
    14401 ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa ctccaggagt ttcaggacag
    14461 tctgaaaaag gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat
    14521 ggatgaacta cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatcctga
    14581 aaaggcgagc gatgaagcgc acaccatcca taacccggtg gtgaaagata atgaagctgc
    14641 gcatgagggc gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg aacagaagcc
    14701 agaaaccacg ccagagccgg tggtaaaacc tgctgcggac gcggaaccga aaaccgctgc
    14761 accttcccct tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt
    14821 atcacgcatc tgattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg
    14881 atattcctgt gtctggtcta tttcgccaat gacatctatc acctggtatc cgcgccattg
    14941 atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt
    15001 acgccgatca agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat
    15061 caggtgtggg catttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg
    15121 ctgctggttt ccagctctct gctgttttat atcggcatgg cattcgccta ctttgtggtc
    15181 tttccgctgg catttggctt ccttgccaat accgcgccgg aaggggtgca ggtatccacc
    15241 gacatcgcca gctatttaag cttcgttatg gcgctgttta tggcgtttgg tgtctccttt
    15301 gaagtgccgg tggcaattgt gctgctgtgc tggatgggga ttacctcgcc agaagactta
    15361 cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg tcgggatgtt gctgacgccg
    15421 ccggatgtct tctcgcaaac gctattggcg atcccgatgt actgtctgtt tgaaatcggt
    15481 gtcttcttct cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct
    15541 gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata
    15601 tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc
    15661 gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa tgggctactc atcaccggca
    15721 ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattcg tcctgttggt
    15781 caacggcggg cgtacatcct cacgacagca gccagtggca agctgcgact gaagaagcga
    15841 ttattgagct ggctgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta
    15901 accgcaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgctcag ctacgcattg
    15961 ccgcagaatt aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga
    16021 cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca
    16081 cacgcgaaga gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg
    16141 tttgcgatga acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga
    16201 aattgctgat cgaaacagat gcgccgtatc tgctcccgcg cgatctcacg ccaaagccat
    16261 catcccggcg caacgagcca gcccatctgc cccatatttt gcaacgtatt gcgcactggc
    16321 gtggagaaga tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa acactgtttg
    16381 ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat
    16441 taataagatt aagcaatagc atggagcgag cctcaccatc gggttcggtg aaaatggcct
    16501 gaaagccttc gaacgcgcct tcggtaataa tcaccttatc acccggataa gggggggccg
    16561 gatcgacaat gtctttcggt ttatataccg atagctgatg aatcaccgcc gatgggacta
    16621 tcgctggcga cgcgccaaag cgcacaaagt ggctgacgcc acgcgtagcg ctgatggtcg
    16681 tggtatgaat cacttctgga tcaaattcca caaacaggta gttagggaat aacggttcac
    16741 tgactgcggt acgtttaccc cgtacgattt tttccagggt gatcatcgga gcgaggcaat
    16801 taaccgcctg tctctcaagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca
    16861 gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa
    16921 agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta
    16981 atttaacaaa tttacagcat tgcaaagatg aacgccgtat aatgggcgca gattaagagg
    17041 ctacaatgga cgccatgaaa tataacgatt tacgcgactt cctgacgctg cttgaacagc
    17101 agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg
    17161 ctgaccgcac tctgcgtgct ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact
    17221 caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatggggc
    17281 aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg
    17341 agccgccaaa aggtttccgc gacctgtttg ataaactgcc gcagtttaag caggtgttaa
    17401 acatgccgac aaagcgactg cgtggtgcgc cctgccaaca aaaaatcgtc tctggcgatg
    17461 acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc gcgccgctga
    17521 ttacctgggg gctgaccgta acgcgcggcc cgcataaaga gcggcagaat ctgggcattt
    17581 atcgccagca gctgattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg
    17641 gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg
    17701 tggcgctggg tgccgatcct gccacgattc tcggtgcagt cacccccgtt ccggatacgc
    17761 tttcagagta tgcgtttgcc ggattgctac gcggcaccaa aaccgaagta gtaaagtgta
    17821 tttccaatga cctcgaagtg ccagccagtg ccgaaatcgt cctggaaggg tatatcgatc
    17881 ctggtgagat ggcgccggaa gggccgtatg gtgaccacac aggttactat aatgaagtcg
    17941 atagtttccc ggtgtttacc gtgacgcata ttacccagcg tgaagatgcg atttaccatt
    18001 ccacctatac cgggcgtccg ccagatgagc ccgcggtact gggcgtggcg ttgaacgaag
    18061 tgtttgtacc gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg
    18121 aaggctgttc ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc ggacacgcga
    18181 agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga
    18241 tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacca
    18301 cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg
    18361 attttgcctc gcctgtctct gggctgggtt caaaaatggg gctggatgcc acgaataaat
    18421 ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtca
    18481 cgtatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat
    18541 gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct
    18601 gtaaagtgac ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc atcgtgccag
    18661 acgcggcctt ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca
    18721 aacgtccgtt ctcaatggcc tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg
    18781 gcgcttctga aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagatcatc
    18841 aaatcgtggt cgacattccc cacggagaag cgtggctgcg cgatgatgaa gagcgtccga
    18901 tgattttgat tgcgggcggc accgggttct cttatgcccg ctcgattttg ctgacagcgt
    18961 tggcgcgtaa cccaaaccgt gatatcacca tttactgggg cgggcgtgaa gagcagcatc
    19021 tgtatgatct ctgcgagctt gaggcgcttt cgttaaagca ccctggtctg caagtggtgc
    19081 cggtggttga acaaccggaa gcaggctggc gtgggcgtac tggtaccgtg ttaacggcgg
    19141 tattgcagga tcatggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga
    19201 tggcgaaaat tgcccgtgac ctgttttgca gtgagcgtaa tgcgcgggaa gatcgcctgt
    19261 ttggcgatgc gtttgcattt atctgagata taaaaaaacc cgcccctgac aggcgggaag
    19321 aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg gcaagtggtt
    19381 aaacccgctc aaacaccgtc gcaataccct gacccagacc gatacacatc gtcgccagac
    19441 caaactgaac gtctttgcgt tccatcagat tcagcagcgt ggtgctgata cgcgcaccgg
    19501 aacaacccag cggatgaccc agcgcgatcg caccaccatt gaggttgatc ttctcgtcaa
    19561 tctgctccat tagcccgaga tctttaatgc acggcaggat ctgcgcggca aaggcttcgt
    19621 tcatctcaaa cacgccgata tcgctggcag aaagccccgc ttttttcagc gccagcttcg
    19681 aggccggaac cgggccgtaa cccataatcg atgggtcaca accaacgacc gccatcgaac
    19741 gcacacgagc gcgcggctta agacctaatt catgggcgcg gctttcactc atcaccagca
    19801 tggcagctgc gccatcggaa agtgcagaag atgtgcccgc cgttaccgta ccgtttactg
    19861 gatcaaacgc cggacgcagc gtggcgaggg cttccacggt ggtttccggg cgaatcactt
    19921 cgtcgtaatt aaactgcttc aggacgccgt cggcatcgtg accaccagtt gggatgattt
    19981 catttttaaa tgcggccgac tgcgtggcgg cccaggcgcg ggcgtgtgac cgcgcggcaa
    20041 aggcatcctg catttcacgg ctgataccgt gtatacgcgc cagcatttct gccgttaagc
    20101 ccatcatgcc cgccgctttg gcgacattgc ggctcaggcc ggggtgaaaa tcgacgccgt
    20161 gactcatcgg cacatggccc atatgctcca cgccgccaac cagacatgcc tgcgcatcgc
    20221 cagtcatgat cattcgtgct gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat
    20281 tgacggtaac cgccgggaca gagtgcggca cttctgccag cagcgccgcg ttacgggcga
    20341 tgttaaaacc ctgctccagc gtctgttgca cacaacccca gtaaatatcg tcgagggccg
    20401 ccgcttccag cgccgggtta cgcgccagca ggctacgcat taaatgagcg gagagatctt
    20461 ccgcacgcac gttacgaaaa gcaccgccct tcgaacggcc catcggggtg cgaattgcat
    20521 cgacaatgac aacctgttcc attgtgattc cttaagccgt tttcaggtcg ccaaccggac
    20581 gggctggctc aaccggagga tagtacggtt cgttatgacg cgctttatta cgcagacctt
    20641 ccggcacttc atacagcggg ccgaggtgct gatattgctg tgccatatcg aggtattttg
    20701 cgctaccgag ggtgtccagc cagcggaacg cgccgccgtg gaacggaggg aagcccaggc
    20761 cgtagaccag cgccatatcc gcttccgccg gagtggcgat aatgccctct tccagacagc
    20821 gcaccacttc gttgaccatc gggatcatca tgcgggcgat aatctcttct tcgctgaaat
    20881 cgcgcttcgg ttggctcact tctgccagca ggtcttcaac ggcggcgtct tcttctttct
    20941 tcggcttacc tttgctgtct tctttataac gccagaaacc gaggccgttc ttctgaccaa
    21001 agcggttggc atcaaacagc gcgtcgatgg catcgcggta atctttctgc atccgctgcg
    21061 ggaagcctgc tgccatgaca gcctgagcgt gatgcgcggt atcaatgccc acaacgtcca
    21121 gcagatatgc cgggcccatc ggccagccaa actgtttttc catcactttg tcgatcttgc
    21181 ggaaatccgc gccgtcgcgc agcagctggc tgaaaccggc gaaatacggg aacagcacgc
    21241 ggttaacaaa gaagccgggg cagtcgttaa ccacaatcgg cgtcttgccc atcttgctcg
    21301 cccaggcgac aactttcgcg atggtttcgt cggagctttt ctcgccgcga ataatttcta
    21361 ccaacggcat tcggtggacc gggttaaaga agtgcatccc gcagaagttt tccgggcgtt
    21421 ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt gttagacgcc agcacggtat
    21481 cctggcgtac tttttgttcg gtttctgcca gtacggcttt tttcactttc gggttttcaa
    21541 caaccgcttc taccacaata tccacgcggt caaatccggc gtagtcgagc gttgggtgga
    21601 tcgtggagat cacgccagcc agtttcagac catcgatctt gccgcgctca agctgcttgt
    21661 tcagcagttt cgcggcttcg gtcatgccga gggttaacga cttgtcgttg atatctttca
    21721 tgacaaccgg cacgcctttc cacgcggact ggtaagcgat accgccaccc atgatgcctg
    21781 cgcccagcac agcagcctgt ttcggggttt caacgtcttt ggtgagtttc ttcgctttgc
    21841 cttttacata ttgatcgtta aggaaaatgc cgaccagtgc gcgagcttcg ttggtatgcg
    21901 ccagcgggac aaaacttttg ttttccagat ttaaggcttc ttcacgacca aaacgggccg
    21961 cagcttcaat ggtttttact gcggtgatgg gggccggata atgtttcccc gccgtttgcg
    22021 cgaccattcc tttagcgatg gtgaagctca tggtggcttc aatcttgctc agttttagcg
    22081 gttccagctt cggctgacgt ttcgctttcc agtcgaggtc gccgttaatg gcctggcgta
    22141 aaatcgccat cgccccttca atcagttttt ctgctttgac tacgccatcc accagaccga
    22201 ttttcagcgc ctgatccgcg ccgacatctt taccggcggc agtgatttcc agtgcactgt
    22261 cagcgcccag catacgtggc atacgtacag aaccgccaaa gcccggcatg atgcccagtt
    22321 tggtttccgg cagaccgatg cgcagatccg gcgtcgccag acgataatcg gtcgccagca
    22381 cgcattcgca gccaccaccc agcgcatagc cgttgacggc agcaatggtc ggcaccggca
    22441 gatcttccag gcgattaaac acgctattgg caaagtgcag ccactgactt aactgttctt
    22501 caggaacgag gaacagggac aaaaattcgg tgatatcagc accgacgata aaggctgctt
    22561 tgttcgaacg cagcagcagc ccttttagat ctgattgctg ttccagcacg ccgatggcct
    22621 cgccgaggct ggcgacggtc gcagtgtcga gtttattaac tgaacctggg gcatcaaata
    22681 ccagttcggc aatgccatct tccagccagt caaggtacag ggtgtcgcct ttgtaaagca
    22741 tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca gatgcatcgg agtgtgtgtt
    22801 ttgtgttaaa aatatgcaaa taaaagatta aagaaatgcc gatctgatca cgctcggcag
    22861 aaatcacgct ctggatgaac gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga
    22921 agggtaaaaa atggaatcac tggcctcgct ctataaaaat catatagcta ccttacaaga
    22981 acggactcgc gatgcgctgg cgcgcttcaa gctggatgca ttacttattc actccggcga
    23041 gctgttcaac gtttttctcg acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa
    23101 agcgtgggtg ccggtaactc aggtgccaaa ctgctggttg ctggtggatg gcgtgaacaa
    23161 gccgaaactg tggttctatc tgccggttga ttactggcac aacgtcgaac cgctgccgac
    23221 ctccttctgg actgaagatg tggaagtgat cacgctgccg aaagccgatg gcattggtag
    23281 cctgctgcct gctgcgcgcg gcaatatcgg ttatatcggt ccggtgccgg aacgtgcgct
    23341 gcaactgggt attgaggcca gcaacatcaa cccgaaaggg gtgatcgact acctgcatta
    23401 ttaccgctcc ttcaaaaccg agtacgaact ggcctgtatg cgtgaagcgc agaaaatggc
    23461 ggtcaatggt catcgtgcgg cagaagaagc gttccgttct ggcatgagcg agttcgatat
    23521 caatattgcc tatctgaccg cgaccggtca tcgtgatacc gacgtacctt acagcaacat
    23581 tgtggcgctt aacgaacacg ctgcggtgtt gcattacacc aaactggacc accaggcacc
    23641 ggaagagatg cgcagcttcc tgctggatgc cggggccgaa tataacggct atgcggctga
    23701 cctgacccgt acctggtcgg caaaaagcga caacgactac gcacagctgg tgaaagacgt
    23761 aaatgatgaa caactggggc tgatcgccac catgaaagca ggcgtcagct atgtggatta
    23821 ccacatccag ttccatcagc gcatcgccaa actgctgcgt aaacatcaaa tcatcaccga
    23881 tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga ccgtttatgc cgcacggtat
    23941 tggccatccg ctgggcctgc aggtgcatga cgtcgccggt tttatgcagg atgatagcgg
    24001 tacacacctc gcggcaccgg caaaatatcc gtacctgcgc tgcacccgta ttctccagcc
    24061 gggcatggtg ttaaccatcg aaccgggtat ctacttcatt gaatcgcttc tggcaccgtg
    24121 gcgtgaaggg cagttcagca agcacttcaa ctggcagaaa attgaagcac tgaaaccgtt
    24181 cggcggcatt cgtatcgaag acaacgtggt gatccacgaa aacaacgtgg aaaacatgac
    24241 ccgggatctg aaactggcgt gatggaaagc tggttaattc ctgcggcacc ggtcacggtc
    24301 gttgaagaga tcaaaaagag ccgcttcata acgatgttgg cgcataccga tggcgttgag
    24361 gcggcgaaag cgtttgttga atcggtgcgg gcagaacacc ccgatgcccg ccaccattgc
    24421 gtggcgtggg tggcgggtgc gccggatgat tctcaacagc tgggtttctc tgacgacggg
    24481 gagccggcgg gaacggcagg taaaccgatg ctcgcccagc taatgggcag cggcgtcggg
    24541 gaaattaccg ctgtggtagt gcgctactac ggcggcatat tgcttggcac cggtgggtta
    24601 gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc agctaacgac ccaacgcaag
    24661 acgccattaa ccgaatatac tttgcaatgt gaatatagtc agttaaccgg cattgaagcg
    24721 ttgctggggc agtgtgacgg caaaattatc aacagtgatt atcaggcatt cgttctgctg
    24781 cgggtggcgc ttccggcggc gaaagtggct gaattttccg caaagctggc ggattttagc
    24841 cgtggttcat tgcaattgtt agcgattgaa gaataatccc cacttcgttt tgcagaacta
    24901 aggaagcggc agagatgcat tttcgcgcca ttacccgaat cgttggacta ctggtcatct
    24961 tattttcagg gaccatgatt atccctgggc tggtagcact catctaccgg gatggagcgg
    25021 gccgcgcttt tacccagacc ttttttgtcg ccctcgccat tggctctatg ctgtggtggc
    25081 cgaaccgcaa agagaagggc gaactgaaat cccgtgaggg gtttctgata gtggtgctgt
    25141 tttggaccgt gctgggtagt gtcggtgcgc tcccttttat cttctcggaa agcccgaacc
    25201 tcacgattac cgatgcgttt tttgaatctt tctctggcct gaccaccacc ggggccacta
    25261 cgctggtggg gctggattcg ctccctcatg ccatcctctt ttatcgccag atgctgcaat
    25321 ggtttggcgg gatggggatc atcgtgttag cggttgcgat actgcctatc ctcggcgtgg
    25381 gtgggatgca gctctatcgc gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc
    25441 cgcgaattgc ggaaacggcg aaaactctgt ggttgattta tgtcttgctg accgtcgcct
    25501 gtgcgctggc gttgtggttt gccggaatgg atgcctttga tgccatcggc catagctttg
    25561 cgactatcgc tattggcggc ttctcgacac atgatgccag tatcggttat ttcgacagtc
    25621 cggctattaa cactatcatt gctatcttcc tgctgatctc cggctgtaac tacggtctgc
    25681 acttttcact gttaagtggg cgtagtctga aggtttattg gcgcgatccg gaatttcgca
    25741 tgtttatcgg cgtacagttt acgctggtgg ttatttgtac actcgtactg tggtttcata
    25801 atgtctacag ttcggcgctg atgacaatta accaggcgtt tttccaggtg gtatcgatgg
    25861 cgacaaccgc cgggtttacg actgacagca ttgcccgctg gccgctcttt ttgccggtac
    25921 tgcttttatg ttcagctttt atcggcggtt gcgccgggtc aacgggcggt ggcctgaaag
    25981 tgatccgcat cctgctgctg tttaagcagg ggaaccgtga gctgaaacga ctggtgcatc
    26041 cgaacgccgt ctatagcatt aagctgggga atcgcgcact gccggaacgt atcctcgaag
    26101 ccgtttgggg atttttctcc gcctatgcat tggtgtttat tgtcagtatg ctggcgatta
    26161 tcgccacggg cgtggatgat ttttctgcct ttgcctcagt tgttgcgaca ttgaataacc
    26221 tggggccagg gcttggcgtg gttgctgata actttaccag tatgaacccg gtggctaaat
    26281 ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt ctttacattg ctggtgctct
    26341 ttaccccgac tttctggcgt gaatgatgga gtaatacgtg aaaacattaa ttcttttctc
    26401 aacaagggac ggacaaacgc gcgagattgc ctcctacctg gcttcggaac tgaaagaact
    26461 ggggatccag acggatgtcg ccaatgtgca ccgcattgaa gaaccacaat gggaaaacta
    26521 tgaccgtgtg gtcattggtg cttctattcg ctatggtcac taccactctg cgttccagga
    26581 atttgtcaaa aaacatgcga tgcgactgaa ttcgatgccg agcgcctttt attccgtaaa
    26641 cctggtggcg cgcaaaccgg agaagcgtac tccacagacc aacagctacg cgcggaagtt
    26701 tctgatgaac tcgcaatggc gtcccgatcg ctgcgcggtc attgccgggg cgctgcgtta
    26761 cccacgttat cgctggtacg accgttttat gctcaagctg attatgaaga tgtcaggcgg
    26821 tgaaacggat acgcgcaaag aagttgtcta taccgattgg gagcaggtgg cgaatttcgc
    26881 ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa taagcataaa gaataaaaaa
    26941 tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg ccggaataac tccctataat
    27001 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga
    27061 gaattccgac agagaaagca aaaataaatg cttgactctg tagcgggaaa gcgtattatg
    27121 cacaccc
//
LOCUS       DAJTYG010000050        26945 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTYG010000050 DAJTYG010000000
VERSION     DAJTYG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.050"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(194..1447)
                     /locus_tag="OO568_002260"
                     /pseudo
     CDS             complement(194..1447)
                     /locus_tag="OO568_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1554..2447
                     /locus_tag="OO568_002261"
     CDS             1554..2447
                     /locus_tag="OO568_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6641601.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYLDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2582..3802
                     /gene="mlc"
                     /locus_tag="OO568_002262"
                     /gene_synonym="dgsA"
     CDS             2582..3802
                     /gene="mlc"
                     /locus_tag="OO568_002262"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6641602.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3927..4622
                     /gene="bioD"
                     /locus_tag="OO568_002263"
     CDS             3927..4622
                     /gene="bioD"
                     /locus_tag="OO568_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6641603.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4575..5855)
                     /gene="clcB"
                     /locus_tag="OO568_002264"
     CDS             complement(4575..5855)
                     /gene="clcB"
                     /locus_tag="OO568_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6641604.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYKVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGGLIVG
                     LLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVFTPTL
                     FIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEMTGEY
                     QLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6014..6628)
                     /gene="dmsD"
                     /locus_tag="OO568_002265"
     CDS             complement(6014..6628)
                     /gene="dmsD"
                     /locus_tag="OO568_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6641605.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6671..7525)
                     /locus_tag="OO568_002266"
     CDS             complement(6671..7525)
                     /locus_tag="OO568_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6641606.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7527..8144)
                     /gene="dmsB"
                     /locus_tag="OO568_002267"
     CDS             complement(7527..8144)
                     /gene="dmsB"
                     /locus_tag="OO568_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6641607.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDNPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(8155..9279)
                     /locus_tag="OO568_002268"
                     /pseudo
     CDS             complement(8155..9279)
                     /locus_tag="OO568_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            9479..10204
                     /locus_tag="OO568_002269"
     CDS             9479..10204
                     /locus_tag="OO568_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641608.1"
                     /translation="MNTNEVSVINLDQLVSMSSYDFLTKVINPARVEAGEKPVRNNDF
                     VSRIEDELDGQNVAYEIFVSFGQDVKSYTLNTDQLLLVGMRESKAVRRKVLAYTGATP
                     TEVIKALDLKLTSSKMLTASVLTGYLKTLCRPNPFYTKRVTEFGTTDIDLSTGRPRAI
                     FNKKGETNGYYRLQPQPNDKEPTIKILRKGYDWLKDNWKDIAHDMRTSNIKVVRRLVD
                     SHLNGKATKSQKAAAGKGSDYGF"
     gene            10451..10672
                     /locus_tag="OO568_002270"
     CDS             10451..10672
                     /locus_tag="OO568_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641609.1"
                     /translation="MTTKQHIDPRTPIGKATLRYRGLPTRHLLSMLGMGVEDPERPFY
                     SRDELIDLLVDRDLNNQLRRAFAKLDATH"
     gene            10774..11136
                     /locus_tag="OO568_002271"
                     /pseudo
     CDS             10774..11136
                     /locus_tag="OO568_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11209..12377
                     /locus_tag="OO568_002272"
     CDS             join(11209..11458,11458..12377)
                     /locus_tag="OO568_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HCR6641610.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC"
     gene            12789..12965
                     /locus_tag="OO568_002273"
     CDS             12789..12965
                     /locus_tag="OO568_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641611.1"
                     /translation="MVNLPPELGSPDVWAGGGVGLIAGLLLRYVLVRVLVVFKLFGRT
                     WTLDLKDNDHTEPR"
     gene            complement(13430..14098)
                     /locus_tag="OO568_002274"
     CDS             complement(13430..14098)
                     /locus_tag="OO568_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6641612.1"
                     /translation="MSHPIINVGRYHGGSDDWRTPYRLFHNLHREFNFSLDGAATEYD
                     ALLPRFTDDIHNQSWVGERVFVNPPFSMAEKFLLKAPEADVCVCLVPLRSKTTYWLRC
                     VYTNPFLHEIRTLHRAVKYLPPAGKRGVVVRSPFPSCVLIYRNTPRKPKVEVTQTIYC
                     GDTMLLLAVVNRGGLRGRPHMHDAQTLDRLIKMYDQGQPIKSIAESLRIPLSTTYRIV
                     QRLG"
     gene            complement(15714..16433)
                     /locus_tag="OO568_002275"
     CDS             complement(15714..16433)
                     /locus_tag="OO568_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641613.1"
                     /translation="MLFMSDAPVKFRIDYILSQEYFYAHYLLAPVFFGGVLAVITPYA
                     QWLLSLAQKWATDKHNENVYLTKEKEYLDSIRLTGLKVRAAREEEKENAKIDADIKAE
                     VERGKREELVTEELQTEKKLIQKEINNLKQLVSKEKQTIENIEIEKERLQDLIVASLE
                     VMDDFFKVDNSRSLQQLKSRVEELLTVSDIEASTIRNALRQKKELTSSQTMKMFDMVE
                     AKIKKEKEKGNDIESDELINQ"
     gene            16638..16988
                     /locus_tag="OO568_002276"
     CDS             16638..16988
                     /locus_tag="OO568_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR6641614.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            17041..17436
                     /locus_tag="OO568_002277"
     CDS             17041..17436
                     /locus_tag="OO568_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6641615.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAMEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            17477..18220
                     /gene="cmoA"
                     /locus_tag="OO568_002278"
     CDS             17477..18220
                     /gene="cmoA"
                     /locus_tag="OO568_002278"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6641616.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     NAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGSAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            18217..19188
                     /gene="cmoB"
                     /locus_tag="OO568_002279"
     CDS             18217..19188
                     /gene="cmoB"
                     /locus_tag="OO568_002279"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6641617.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(19353..21695)
                     /gene="torZ"
                     /locus_tag="OO568_002280"
     CDS             complement(19353..21695)
                     /gene="torZ"
                     /locus_tag="OO568_002280"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6641618.1"
                     /translation="MALTIREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAAMLGQIGTPGGGFGFSYHYSNGGNPMRVGGVLPEMSAAIAGQASEAADD
                     GGMTAIPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAW
                     QKPEMIVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFE
                     ARNDFDVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFW
                     QQNKLIEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHP
                     TWLAPDEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAAR
                     FGIANGDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTA
                     DIPSSQLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
     gene            complement(21720..22820)
                     /locus_tag="OO568_002281"
     CDS             complement(21720..22820)
                     /locus_tag="OO568_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6641619.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(23208..23954)
                     /gene="cutC"
                     /locus_tag="OO568_002282"
     CDS             complement(23208..23954)
                     /gene="cutC"
                     /locus_tag="OO568_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6641620.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGGLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(23968..24534)
                     /locus_tag="OO568_002283"
     CDS             complement(23968..24534)
                     /locus_tag="OO568_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6641621.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            24750..26483
                     /gene="argS"
                     /locus_tag="OO568_002284"
     CDS             24750..26483
                     /gene="argS"
                     /locus_tag="OO568_002284"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6641622.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRSLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            26660..>26945
                     /locus_tag="OO568_002285"
     CDS             26660..>26945
                     /locus_tag="OO568_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HCR6641623.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDTQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLK"
BASE COUNT         6968 a         6670 c         6471 g         6836 t
ORIGIN      
        1 tagaataact gcgcatcagt aataaatgac acacagcaaa atgaatccgt ttatttgggt
       61 acttataatc ctgatgacgc tagatgcgct gcgggaattg gctggcgctt cgtctatttt
      121 aggatggcta ttaacgttgg tttgagcagg caataagtcc ggacgggtat ttaccgcagt
      181 ccggacttat ttttcaggcg tgcagacgac gatgcaaacg cgccccgacc agcagagcaa
      241 tgaccagcat cagagcaata aatgcgccga cgccgttcca gccatagtta tgccagaaaa
      301 caccacccag cgtcccggca atactcgacc ccagatagta actgaacaga tacagcgagg
      361 aggcctggcc tttagcgcgt tttgcgcggg ggccgatcca gctgctggct actgagtggg
      421 ctgcgaagaa tccggctgag aagagtaaca ttccggcaaa gatcagccac agcgagctaa
      481 ataaggtcat cagtaaacca aacggcataa ctcccgtcga aaacaacatc accggaccgc
      541 gcccatagcg ggtggtcatg gttccggctt tgggtgagct ccatgtaccg gtcaaataag
      601 ccagcgataa taagccaacc acggcctgac tgacatgcta gggggaaagc atcaaccgat
      661 agccgatgta attaaacagc gtgacgaacg accccatcag caaaaagcct tctgcgaaca
      721 ataacggtaa tccccggtca cgccagtgca gacgaaagtt gataaataac gtcttagggc
      781 gcagcgaagt cgggcaaaaa tggcgtgatt cagggaggat tttccagaac atcaacgcct
      841 aggccagcgc gaaacaaccg attgccgcca gagcaattcg ccagttgaaa aagtccgtga
      901 agacaccact aattaagcgt ccgctcatgc cgccaattga gttgccgctg atatacaacc
      961 ccatcgaaaa ggccacgaaa ctggggtgga tttcctcgct aagataagtc atgccaacag
     1021 ctgccacgcc acttaacgaa agcccaatca aggcgcgcat aatcaaaatg ccgtgccagc
     1081 tggtcatcat tgtcgaaagt aacgtacaaa tggaggccaa cagtagcgcc gtgaccatca
     1141 ctggtttgcg accaatggca tcggatagcg ggccagtaaa tagcaaaccg atagccaaca
     1201 tcgccgtgga aatggacagt gaaatactac tgttcgcggg cgttaagcca aactcctgcg
     1261 aaagcaccgg aaggataggc tgcacacaat agagaagtgc aaaggttgcc agtccggcag
     1321 agaacagcgc cagggtgacg cgcataaatt gcggcgtacc gcgtttaata aattgatttg
     1381 gctgagaaat gctttgcttg tcagtgtcgc ttgccggagc gccatcaaca gttgtagtac
     1441 ggctcacttg aaatccttgc taaatatgcc tgtggatcag gcttatacat agggtaggaa
     1501 aatcgaattg ttctgtctaa tatattaata atctcaaata agatgtttta aatatgaata
     1561 ttgaacttcg tcatctgcgt tactttgttg ctgttgcgga agagctgcat tttgggcgcg
     1621 cggctgcccg cctgaatatt tcgcaaccgc cgctaagtca gcagattcag gcactggagc
     1681 aacaaatcgg tgcccgactg ctggcacgaa ctaatcgcag tgtattgctg acggcagcag
     1741 gaaaacagtt tcttgcagat agtcggcaaa tcctgtctat ggtggatgac gctgccgctc
     1801 gcgctgaaag actgcatcag ggtgaagcgg gggagttgcg tattggtttt acttcgtccg
     1861 ctccttttat tcgggcggtg tccgatacgt tatcgctgtt tcgccgtgat tatcttgatg
     1921 tccatttaca aacccgcgaa atgaacactc gcgagcaaat cgctccgctc attgaaggaa
     1981 cgctggatat gggattgctg cgtaacacag cgttaccgga gacgcttgaa cacgcagtca
     2041 tcgtccatga accgcttatg gcgatgatcc cgcacgatca tcccctggca aataatccga
     2101 gtgtaacact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
     2161 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
     2221 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
     2281 caattttgcc tgcgtcattt aaacgtgttc agctcaacga aatgcgctgg gtgccgattg
     2341 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
     2401 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
     2461 gaaaaagctc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
     2521 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
     2581 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
     2641 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
     2701 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
     2761 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
     2821 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
     2881 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaactgg cgttaaaaga
     2941 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
     3001 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
     3061 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
     3121 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
     3181 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
     3241 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
     3301 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
     3361 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
     3421 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
     3481 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
     3541 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
     3601 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
     3661 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
     3721 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
     3781 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
     3841 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
     3901 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
     3961 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
     4021 aaacggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
     4081 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
     4141 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
     4201 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
     4261 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
     4321 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
     4381 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
     4441 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
     4501 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
     4561 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
     4621 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
     4681 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
     4741 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
     4801 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
     4861 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
     4921 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
     4981 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
     5041 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
     5101 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
     5161 atcagcgcat agtcacgagc ctgaaccgtc actttgtaga gtaacgcgtc gctatgatta
     5221 atcagattgc taaccagcaa tgccacgacg gcggaaataa tcaccgggcc gagagaggcc
     5281 aacatcatag tgccaaacag cacttcggct ataaataaac tgccagcaag cggggcacga
     5341 taggccgcag ccattcccgc cgcggcccca caggcgatcc ataatttcca ttcctggcgt
     5401 ggcgtaaaac gttgggcaaa acaggaggcg gcaagggcag ctaaaagaat catcgcacct
     5461 tcgcgaccaa ttgcactgcc gctggttact accagcagcg aggcaagcga tttaaccagg
     5521 cttgctgcgt aatcgaactg tccatcggtt tgcaacgctt ccatgtaatc ggtcggcgca
     5581 tgagggcgtt gttgggtaaa tttctgccag cccatcagca acaaacccgc cgccagtccg
     5641 ccgagcgccg gagttagcag ccgtcgccag ggggaaaggt ttgtcgctgc attgaccaga
     5701 ctgccggagt cattattgag gaacaaccac tccagtagca gcatcgcatg acgaaacccg
     5761 gcaacggcaa agacagcgag aataccgacg actgttgcga taagcagacg gcggaacata
     5821 gtgcgcagat ccggatagat gtgtagatga tgcatgagca gaaatgggca atgaaacacg
     5881 ggcgactatt ttgcggcaaa agaggccaac cgcaaaagca gtgcgtgctt taagagataa
     5941 aaaaacgtga cattgtaacg tattattgcc gaatcgcgcg taatcgtcac catccggcaa
     6001 tattacggtg atcctatcga aagagtggtt taaccgcgac aggaatcaac agttgtgatt
     6061 gccattgcgc cagcgttaag cgagccagtt cacccagtgc acggtagaaa gggtgttctg
     6121 ctttttcgat aaaaacatca agaaaacgtg ttgaccacgg aaaaaggtgc catgccagca
     6181 attcttcgca ttctgtctgg cgaccattct ccgccaacca cgcagccatc aacagcagtg
     6241 aaccaaaatg atcttccggt tcgttttgct tcatttcaaa ctgaatgcct ttctcgcgca
     6301 tccactgacg aagtgccaat gttgaatcgc caaacagcac agattcgcga tccagccaga
     6361 ccgaccccca cggcggagac ggcagtgccc acgggccgac aaacaaacgc tgccaggcct
     6421 gggcgtgagt ctcttcacac tgtgtctgaa aagccgtcac cagcggcgct aatgacgctt
     6481 ctggtagagg ccactgagtt tcccagccgt cactggtgag taccgcaacc agaggtgcgg
     6541 cttccgcact ctctggagcg taataaaaca gcgctcccaa cacgcgcgcc gcgacagaaa
     6601 aattatcttg ctgtgaaaaa tgggtcattc ctaacatcct gaaatgtgcg ggttgccccg
     6661 caccaatttg ttaacctgca atagccattc ctactgtcat gtgcaggcca taaaagagca
     6721 cacgcccgat catctcacca acaagtatga ggattagccc cagaacaagt ccggcaacgt
     6781 gaggttcacg gcgacgaatg agcggacaaa gccagcatcc aagacctgcg cataacaata
     6841 ccacacgcca gacctgtaag gaggcgtaat ctggtaccag tgcgctggct tgttgcacgg
     6901 atgagtgaat agacgccaga gacaaacctt gcagcacaat cacctcggca caagcgatca
     6961 gcgccagaac gctaatgatg gcaaatggtg tggtattaaa tgtaacgcgg gctgcccgca
     7021 gaattgcggc agcaagtatt ggaccgctca acaataccgt caggaagaaa gccagcgtag
     7081 tgtaaccgtt atgccaggtt ggcacggtgt cgatttgata cacgcaggtc atcatccaga
     7141 cgaaaatgac gccgagcgcc atgctgacaa gtaaccagag tttccccaac gcttgcggca
     7201 ttttaccgat gaccgccacc agccaccata gtccgccaac ggcaaaaaat atggaaccgg
     7261 cagcaatctc gttactcaga ccagaagctc cgattcgatt cagcgagtta aacgctcgca
     7321 gtggcgatcc caggtgcatg acagaggcaa taaacccgat gcccatcaac agccagagga
     7381 aaaacatgcc acgaacaatg tgctgacggt ctgcgtcatt tttggcggcg aaccagccaa
     7441 ttccgctaac aatcagcgcg cctactacgc attgacccaa tacggtaaag ataaccagtg
     7501 gccattcatg ccatccattt cccattttac acttcctccg gattagccag ataaccagtg
     7561 gtatcgccgg tcgggcggct gttggcgtta ggtttgataa cgatattggg ttttgtgaaa
     7621 tgcgcgcgcg gcagtggcgc gacggcagcc agagtaccgt gcttctggcg cagctcttca
     7681 attggaccga actcaagtgc acgcagcggg caggattcga cgcatatggg ttgtttcccc
     7741 tcggcgacgc gcgaataaca accatcgcac ttcgtcatgt gccctttttc agcattgtac
     7801 tgtggtgcgc cgtacggaca agccatgtgg cagtagcggc agccgataca aacatcttca
     7861 tcaaccacca caaagccatc ttcacgctta tgcattgcgc cgctggggca gacttttgta
     7921 catgcggggt tatcgcaatg gttacaggag atggagagat aataagcgaa cacgttctgg
     7981 tgccagatgc cattatcttc ctgccagtcg ccgccagcat attcataaat acggcggaaa
     8041 ctgacttccg ggccaagatc tttaaaatct ttgcacgcca gttcgcaggt tttacagcca
     8101 gtacagcggc tggaatcgat aaaaaatcca tattgtgtgg tcatgggcta ctccttaaac
     8161 cttttcgatc tggacaagat tgctgtgcga cgggtttccc tttgctagcg gtgaagggcg
     8221 gtgagaggtc agaatattga tactgccgcc gtgatcgacc cggtcaccaa acatatccgc
     8281 tttaagccac gcaccttgcc cgatggcggt aacgccaggc agaatacgcg gagtcacttt
     8341 tgcggcaatc agcatttctc cattattgtt aaatacccgc acggtatcgc catgacggat
     8401 accgcgtgcc tgagcatcaa tggggttgat ccacacctct tgtgggcagg cctgctgtaa
     8461 cacatcaata ttgccgtagc tggagtgggt acgcgctttg tagtggaagc ccgttaactg
     8521 cagtggatag gttttccgca ggggagcgtc ccagccatca aaacctgagg tatacgcagg
     8581 aatgggatga ataatttcat cttttttcaa ttcccaggta tctgcaatct tcgccagtcg
     8641 ttcagaataa atttcgattt tccccgaagg tgttttcaac gggtttgcct gtggatcttc
     8701 acggaatgcg cggaaagcga cgtagtgttc ttccgggcat tttttcttaa agatcccggt
     8761 cgttttcatc tcctcgtagt cgggcatctc agggttacgt tccttcgttt tcgcatggag
     8821 atatttgatc cattcatgct gactgcgacc ttcagtaaag gtttgataaa cgtctggtcc
     8881 taagcgtttg gcgacttcac tcagcatcca gtagatgggt ttgcgttcaa attttgctga
     8941 ggttgcgggt tgggcgagga tcacatagcc catattccct gcagattcat gagagataag
     9001 gtcttcttgc tctgttggca tcaggtcagg caacaggata tcgcaatact tagccgaggc
     9061 cgtcatgaag tggtcaatgc caacaatcat ctcgcacttg ctgtcatcct gaagcacctc
     9121 atgggtgtga ttgatgtcgc catgttgatt gatcaatgtg ttactggcgt agcaccataa
     9181 aaacttgatg gggacatcca gtttttcttt tccacgaaca ccatcacggg tcgcggtcat
     9241 tttcgtacca tggtcgatgg catctgtcca tgtaaacatc tggggtcctt acagctcgtc
     9301 ctgacatgcg gtctcgcctt atatgcacat ggagaacatg gtcactccag accaacacta
     9361 atcaacatta accctattca ctttcgttaa gcactttttg gtgggtccgt ttggatgcat
     9421 tgaataggtg cggactccac atggagacag aaagtgcttt accaaaggag catcctttat
     9481 gaataccaac gaagttagcg tcatcaacct tgaccagctt gtgtctatga gtagctacga
     9541 tttcttgact aaggtcatca acccggctcg tgtggaggct ggggagaagc ctgtccgtaa
     9601 caacgacttt gtgtcccgta ttgaagatga gctggacggt caaaatgtag cctacgaaat
     9661 tttcgtaagc ttcgggcagg acgttaaaag ctataccctc aacactgacc agcttctact
     9721 ggtcgggatg cgtgagtcta aagctgtgcg tcgtaaggtc ctagcctata caggcgccac
     9781 gcctaccgag gttattaaag ctctggacct taagttgacc agctctaaga tgctgacagc
     9841 tagcgtcctg actggatatc tgaagaccct gtgtcgtcct aacccattct acaccaagcg
     9901 tgttactgag tttggcacta cggatataga cctatcgacc ggacgtccac gagccatctt
     9961 caataagaaa ggtgagacca acggctacta tcgtctacag ccacagccta acgacaaaga
    10021 gcctaccatc aagattctac gcaagggtta tgactggctg aaggataact ggaaggatat
    10081 cgctcatgac atgcgcacat ccaatatcaa ggttgtccgt cgtctggtgg attctcacct
    10141 gaatggtaag gctacaaagt cccagaaagc agcagcgggc aaggggtctg actatgggtt
    10201 ctaagcgact cactgatgaa ctgattatgg agtcaatagc gacaaccata tgtttcattg
    10261 agagacacgg ccctgagcat cctgcggacc ctatcttact gacttctctg cttcggcata
    10321 tacacgaaga gatggcactg agagtcgacg agcgactgcc tctagagata ccagagggtc
    10381 tacctaactt ccaagctcct atcaccagtg aatttgacgt ggatgattgg tctaacgagg
    10441 acttctaaca atgacaacta aacaacatat cgacccacgg acacccatcg gaaaggctac
    10501 tctccgttac cgtggtctgc ctacccgtca cctcctgtca atgctgggga tgggcgtaga
    10561 ggaccctgag cgtcctttct atagtcgaga cgaactgata gacctgctgg tcgaccgtga
    10621 cctgaacaat cagttacgcc gagcgttcgc taagctggac gccactcact aacccctaat
    10681 ttaaaccttt tgagatgacc gatgcgggga gacccgtagg cataactgtg tctgcggtac
    10741 cgatagtctc aactctcaag aggagtaatt actatgtcta gcaaagctag tgcgttattc
    10801 aacaacaatg actggaaact ccatgtcacc gacatcaatc tctacgaaaa cactgtgagt
    10861 ctggacggtc agtcctaccc actgtccttc gctattaaga ccctgattcc gggctacctg
    10921 tcaggactgc ctgccaccag ccgcgagtcc atggaattac tggaagctct ggctgaagct
    10981 ggcgtgacca ttggaaactt cttcagcaat gacctgatga ccgcctacca acgtcgccag
    11041 cagaacaagc gagccgaggc tgagcgtatc gctaaagagc agcgcattca ggctgagcgt
    11101 atgcgtgaag agaacatgac cgatgcagag tggcagtgaa gtggcacact gaatttggcc
    11161 acctgaacag aggtgatatg ctcacctcag aacaacacag gtgctccaat gaaaaaaaga
    11221 aattttagcg cagagtttaa acgcgaatcc gctcaactgg ttgttgacca gaaatacacg
    11281 gtggcagatg ccgccaaagc tatggatgtt ggcctttcca caatgacaag atgggtcaaa
    11341 caactgcgtg atgagcgtca gggcaaaaca ccaaaagcct cccccattac cccggaacaa
    11401 attgaaatcc gtgagctcag gaaaaagcta caacgcattg aaatggagaa tgaaatatta
    11461 aaaaaggcta ccgcgctctt gatgtcagac tccctgaaca gttctcgata atcgggaaac
    11521 tcagagcgca ttatcctgtg gtcacactct gccatgtgtt cggggttcat cgcagcagct
    11581 acagatactg gaaaaaccgt cctgaaaaac cagacggcag acgggctgta ttacgcagtc
    11641 aggtacttga gctacatggc atcagccacg gttcggccgg agcaagaagc atcgccacaa
    11701 tggcaacccg gagaggctac cagatgggac gctggcttgc tggcaggctc atgaaagagc
    11761 tggggctggt cagctgtcag cagccgactc accggtataa acgtggtggt catgaacatg
    11821 ttgctatccc taactacctt gaaaggcagt tcgccgtgac cgagccaaat caggtgtggt
    11881 gcggtgatgt gacctatatc tggacgggta agcgctgggc gtacctcgcc gttgttctcg
    11941 acctgttcgc aagaaaacca gtgggctggg ccatgtcgtt ctcgccggac agcaggctca
    12001 ccatgaaagc gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg gtgatgttcc
    12061 acagcgatca gggcagtcat tatacgagca ggcagttccg gcagttattg tggcgatacc
    12121 agatcagaca gagtatgagc cggcgcggaa actgctggga taacagccca atggaacgct
    12181 tcttcaggag tctgaagaac gaatggatgc cggtggtggg ttacgtaagc ttcagcgagg
    12241 cagctcacgc cataacggac tatatcgttg gatattacag tgcactaaga ccgcacgaat
    12301 ataacggtgg gttaccccca aacgaatcgg aaaatcgata ctggaaaaac tctaactcgg
    12361 tggccagttt ttgttgacca cttcacttgg gtaattcgta gctggtgggg gtgtctgtgt
    12421 agctgtagga gttgtatctg ttacgttgtc tcgatgcctg aagcatcgct ctaaattctc
    12481 tgtcgttcat tcataagcta gtccttataa ttgttaattc tctgttaact tatatagaca
    12541 atactagcat tttcaaagac taatgtcaag cattttttaa catttcatta acatctgtgg
    12601 tataatggtt ctatggggag agtaaaatat aaaggcgtgg ctatgagagt ggtcatatgg
    12661 ttattgcatt gcacacagac ctcttcaatc gctctgtaac gagttaaatg gcctcttcag
    12721 gtcaacatat aaggtcaggt acttttttgt gtcagaatgc gattgagagc gtcttacaag
    12781 gaaaactaat ggtaaatctc cctcctgagc tggggtcacc tgatgtatgg gctggtggag
    12841 gtgttgggct gattgcaggt ctgctacttc gctacgtcct cgtcagagtg cttgttgtct
    12901 ttaaactgtt cggtcgtaca tggacgcttg accttaagga taacgaccac acagaacccc
    12961 gttaaactcc cttcaaaacc ccctgtacgt gatggtatag gggtatcttt agcattttgt
    13021 gctatctatt aataaatagc ttgacataac cggaaaactg aatctatcaa gtattcaccc
    13081 ctctgacagt aatttctaaa aatgcttatt tttcaatgag ttgtaattag ccaatataaa
    13141 ggactgacaa gactcttcac agagaacttt caagactcac taagacacaa ggcgataacc
    13201 ccagacaggg agagcctcct tcttaaccga cttcttaaac aatctttaca gacccccgtt
    13261 caccagtctc aatattttta tttctcgctc ttcgtttttt gctcgtttct attattgagg
    13321 gggtttgaaa aacctctgta aaataaccct tccagtaaaa agcgaataac cccagaaagg
    13381 ggaaatcgca tctttaacat tctcgtaatc agtgctctat atgagaactt cagcccaacc
    13441 tttgaacaat cctgtaagtg gtgcttagag gtattcttag cgattctgcg atgctcttga
    13501 tgggttgccc ttggtcatac attttaatca acctgtcaag cgtctgtgca tcgtgcatat
    13561 gaggtcttcc acgtaaacca cccctgttaa ccacggcaag taaaagcatt gtgtcaccac
    13621 aatagattgt ctgggtaact tcaactttcg gtttgcgggg tgtgttcctg tatatcaaaa
    13681 cgcaacttgg aaacggtgag cgcactacaa cacctctctt tcctgctggt ggaaggtatt
    13741 tgactgctcg gtgtaacgtt cgaatctcat gcaggaaggg gtttgtatat acacaccgaa
    13801 gccagtaagt tgtctttgac ctgagcggaa ccagacatac acacacatca gcttcagggg
    13861 cttttaagag gaacttttca gccatgctaa acggcgggtt aacgaagacc cgttcaccaa
    13921 cccatgattg attgtgaata tcatcggtga accgtggcaa caatgcgtca tactcagttg
    13981 cagcaccatc tagactgaag ttaaactcac gatgcaggtt atgaaataaa cgatacggtg
    14041 tacgccagtc atcagaacca ccatgataac gaccaacatt gattattgga tgcgacacaa
    14101 gcgaagcgcc cctcttagta tatttcttaa tcgtctcttt agcatttagt gctttttgtt
    14161 aagtttagca ctttttgcta aagacaaaag cattgacaaa atggtaggat tgttcacata
    14221 gcccttactt aacagactct taagagcttt gaaacagcct gtttaacaag gtcactacct
    14281 ctggtcttat cgttcccttc tttctaggta gtcatctgtt aagaactttt cagttttatt
    14341 cttaagataa ttgttaagat gatactctcc cttcgggtta tatcgtatct ctcctcccgt
    14401 taaatatccc ttcacagtca gactaaataa cacctgtgta gtttcttggt agaatctttc
    14461 aaggtaactt aactctgagt ttatgaagtt gacctatgag atagactcta ccgaatctgt
    14521 tagtcaattg ttgcttaggt gctgctaagg cttacttagt gggaagagag ggaatatgtg
    14581 accataggaa cccctcctgt taaaagcttc ttgacgttac tatctcgata tgtataatgg
    14641 cttactgtac taggtttact tccaaagcaa ccaagcacac tctctatggc agtgtccagc
    14701 gaggtaaggt cgtgtagggt gcggttcggt ggggtaaggt ttggtgcggt atgggtggat
    14761 tgtcgtagcc acgttaaaaa tcgacttata gggactcttt cgggagtcct ttttagtttg
    14821 tataaaatct gtacagtaac ttttcagagt ctgaaaattt ttataaatat cgtagtaacc
    14881 cctctacatt ccccttcacc gataattttc aaaaatatct acactcaatt gatggctgtt
    14941 aagtacccca gattcacgat atagcccccc ccctttgcaa aagcagttaa aatatctata
    15001 accaaccatt gactttcttc atgatatctg tttagcccca cctcaataag ccatcatcac
    15061 acatcaaagc atctatcaag aaggtttaac ctatcaatag ctgtaacatt ttatagttct
    15121 gtgaagtcct atccagtttt ctctgtaaca gctacaaact tctgtgacct tatgtgtcaa
    15181 tgacttataa gacatgctct caaggtgctg tatagcctct tatcagccaa tacacaaaac
    15241 ccttataaat cagtactgat atgtatacag tatgttttta acataatagc tgttttacgc
    15301 actaataagc acctacgtag attctgtata gagattaaga tgtctctaag tgatttttta
    15361 agaggagata cgaaggaact ataaccatca cttcataact ttcacgtagg taactattaa
    15421 gtaggttgat agtcactttg agggaatatt gatagtgacc aactgcatag acaccgttat
    15481 atagccctgt atatagcact atatgtagtg tctggagagg gacgactaac acgatatagt
    15541 ttaccctgaa aagaggattt acagattctc tatacatttc aataagttat gataaaaagg
    15601 ggctatgtcg atactgtata gaatctgtgt aggattgcat agatacttgg aaaaataccg
    15661 ttaaattgca taggtactaa cctgtttatg tcctgttatt tacaggcata gcattattgg
    15721 ttaattaatt catccgactc tatatcatta cctttttctt tttccttttt aattttagcc
    15781 tctaccatat cgaacatttt cattgtctgt gatgaagtta gctctttctt ttgtcgtagc
    15841 gcgttacgta tggtagaagc ttcaatatca gaaactgtta aaagttcttc taccctactc
    15901 ttcaattgct gtaatgaacg gctattatca actttaaaga aatcatccat tacctcaagt
    15961 gaggcaacaa tcaaatcctg tagtctctct ttttctatct cgatattttc aatggtttgt
    16021 ttttctttag atactaactg ctttaaatta ttaatttctt tctgaatcag tttcttctct
    16081 gtctgtagct cttctgtgac cagctcttca cgcttaccac gctctacttc cgctttaata
    16141 tcagcatcaa ttttagcatt ttctttctct tcttctctgg cagcccgaac ctttaacccc
    16201 gttaacctga ttgaatccaa gtattctttt tcctttgtaa gataaacatt ttcattgtgt
    16261 ttatctgtag cccatttttg agcaagagaa agtaaccatt gagcataagg agtaataaca
    16321 gctagcacac ctccaaaaaa gacaggagca agcagataat gagcgtaaaa atattcctga
    16381 gatagtatat aatctattcg aaacttaaca ggcgcatcac tcataaagag cattgctaag
    16441 ttaggccagt tgaaccatac ccatgcaaga atgatatatc cccataatgg gcttttaatc
    16501 ctatcgttta ttgcttgcat agtagaatct aaccacccat cactcatagc taaccctcgg
    16561 aagtcttatc tttctgtcta tcataaccac aagcatcacc tttaggaacc atctgaaacg
    16621 ggaggtgtat ttagaccttg ctaatcttag acagccgccc ggttcatgca tcacgtccac
    16681 acagtgaagc tattcgcgac gctcaacgaa aaaaacctaa agttccggta catgctgtac
    16741 tgacggcgac aaatccgctg atccgtttta tcggtagtga tgatatgacg caaaaccggg
    16801 aattatttca ggtctggtta caaaaattag cgcagtggca tcagaccact acgccttatc
    16861 tttttttaca tacgccagat attgcccagg ccccggaact ggtacatacc ctgtgggaag
    16921 acttacgtaa aacgcttcca gagatcggag cagttccggc tattccacag caatcttctc
    16981 ttttctgaat ttgccaccta tcatagacag gtgccatcgg ccattttaaa gggagtttgt
    17041 atggtaagcg cgctatatgc cgttttaagt gcgttgttat taatgaagtt ctcttttgat
    17101 gtcgttcgcc tgcgaatgca gtaccgcgtt gcctatggtg acggtggttt tagcgaactg
    17161 caaagcgcta ttcgcattca tggtaacgcg atggaatata ttcccatcgc gattgtgctg
    17221 atgctgttta tggaaatgaa tggcgcagaa acctggatgg tgcatatttg cggcatcgtt
    17281 ttgcttgctg gtcgtctgat gcattattac ggttttcatc accgtctgtt tcgctggcga
    17341 cgttccggca tgagcgccac ctggtgtgcg ctgttgctga tggtgctggc gaatctttgg
    17401 tatatgccct gggagttggt tttctccctg cgttagcgca caatacgcca ctttcttttt
    17461 cccggatttt tacgttatgt ctcaccgcga cacgctattt tctgccccta tcgccagact
    17521 gggcgactgg acctttgatg aacgggtagc tgaagtcttc ccggatatga tccagcgttc
    17581 cgttcccggc tattccaata ttatttccat gattggtatg ttagccgagc gcttcgttca
    17641 acctggtacg caggtttacg atctgggttg ttctctgggc gcggcgacgc tctcggtgcg
    17701 tcgcaacatt catcatgata attgcaaaat tattgccatc gacaactccc cggcgatgat
    17761 tgaacgctgc cgtcgtcata ttaacgccta taaagcccct acgccagtag acgtcattga
    17821 aggtgatatt cgcgatatcg ccattgaaaa cgcatcgatg gtggtgctga attttaccct
    17881 gcaattcctg gaaccttccg agcgccaggc attactggat aaaatttatc aaggactgaa
    17941 cccgggcagc gcgctggtgc tttcggaaaa attcagtttc gaagatgcca aagttggtga
    18001 actgttgttc aacatgcacc acgactttaa acgtgccaac ggttacagcg aactggagat
    18061 cagccagaag cgcagcatgc tggaaaacgt gatgctgacc gattccgtgg aaacccataa
    18121 agcacgcctg cataaagccg gttttgagca tagcgagctg tggttccagt gctttaactt
    18181 tggttcactg gtggcattaa aagcagagga cgctgcatga tcgactttgg taacttttat
    18241 tctctgattg ccaaaaatca tctttcacac tggctcgaaa cgctgcccgc gcagattgct
    18301 aactggcagc gcgagcagca gcacgggctg tttaagcagt ggtccaacac ggtggaattt
    18361 ctgcctgaaa ttaaaccgta tcgtctggat ttattgcata gcgtaaccgc cgaaagcgaa
    18421 gagccactga gcaccgggca aattaaacgc attgaaacgc ttatgcggaa cctgatgcca
    18481 tggcgcaaag ggccgttctc actgtatggc gtcaacatcg ataccgaatg gcgttccgac
    18541 tggaaatggg atcgtgttct gccccatctt tctgatttaa ccggacgcac cattctggat
    18601 gtcggctgcg gcagcggtta tcacatgtgg cgcatgattg gcgcaggcgc gcacctcgcg
    18661 gtggggatcg accctacgca actgtttctg tgccagtttg aagcggtgcg taaactactg
    18721 ggtaacgatc agcgcgcaca tttgttaccg ttaggtattg aacagcttcc ggcactgaaa
    18781 gcctttgata ccgtcttttc gatgggcgtg ctttaccatc gccgttcgcc gctggagcat
    18841 ctctggcagt tgaaagatca actggtgaat gagggtgaac tggtgctgga aacgctggtt
    18901 attgatggcg atgagaacac ggtgctggta ccaggcgatc gttacgcgca aatgcgtaat
    18961 gtctatttca tcccttccgc gctggcgctg aaaaactggc tgaagaagtg tggttttgta
    19021 gatatccgca ttgtggatgt gtgcgttacc actactgaag agcagcgacg cactgaatgg
    19081 atggttaccg aatctctgtc cgatttcctc gacccacatg atccgagtaa gacagttgaa
    19141 ggttatcctg cgcctaaacg cgcggtgctg attgcgcgca agccgtaaag gtctggtatt
    19201 actgccggat gcggcgtgaa cgccttatcc ggcctacaaa cccttgctaa ttcaatctat
    19261 tgcagggact atgtaggcct gataagcata gcgcatcagg cagttttacg tttgcataac
    19321 ctcagcgccc gtttccgggc gctattcact tcttacgcct gaacagcagg cttatcaaat
    19381 gccgttaact tcggtgcgtt acccgtatat ttttcgatat acaccagcgc agagttaccg
    19441 gcacaggcat ttgccagctg cgagctgggg atatccgccg ttaacacgtt cgcactgccg
    19501 tttttacaca agccgttttc cagatctggc catgcacctt catgcacgca taccacgcct
    19561 tttttgatcc cgtcagtcac caccgcgcct gtcaaaatct gaccacgttt gttccagaca
    19621 cgcaccagat cgccattcgc aataccaaat cgagcagcat cttcggtgtg aatagtgatc
    19681 ggttcacgat cggcaaccgc atatttttta cgcagttccg catagttaag ctgactgtgt
    19741 aaacggtgtg ccggatgtgc ggtcagaagc tgcaactgct tctcgtcggc ggtacccttc
    19801 cactcatcag gcgcaagcca ggttgggtgt gccgggcaat ccttatagcc aaatttttcc
    19861 agcgttttgg agtaaatctc aattttgccg cttggcgtac ccagcgcatt tttcaccgga
    19921 tcggcgcgga aatcaccata acgaacgtac tgttcgttct tctcgctgcg gcgcatttcg
    19981 atcagtttat tttgctgcca gaaggcatta aacattggca tagtgacgcg ttgcgcacgg
    20041 gcccctttct gagcggcatc atagaaaaat ttcagccacg ccatttcatc tttaccttcg
    20101 gtatagatct cttttccgcc aggtttgagt aattccgcaa gatcggcaaa cacgtcaaaa
    20161 tcgttacgcg cttcaaattg cggagcaaca gcctgcttca tcggcacaat atgctggttg
    20221 ctgtaatcac cggtcatcgt caagtcattg cgctcaaacg atgtggtgat cggtaatacg
    20281 atatctgcgt gtttggctgc tgcggtccag tagcattcag aaacgacgat catctccggt
    20341 ttctgccagg ctttgatcag acgattagta tcctggtgat gggtaaagtt accgccaccc
    20401 gcccaccaga tcattttgat attcggatac gtttgttctt taccgttatg ctgatatttg
    20461 ccgccaggat tttccagcgc atcgacgata cgggcaacag gaatcgccgt cattccgcca
    20521 tcatcagcgg cctcgctggc ctgtccggca atcgccgcag acatttccgg cagcacgccg
    20581 ccaacacgca tcgggttgcc accattggag taatgataag agaaaccaaa accgccgccc
    20641 ggtgtaccaa tctgccctaa catcgccgcc atcagttcag cgagtttgac tatctgcgct
    20701 tccggtacgc cagtaatttc cgccgcccag accgcgctct tcggcgtatt atcgctctta
    20761 cctgtcagat actcttcaaa ctgcggatac ccggtagtgt atttttcaag gaacacttta
    20821 tcgtgtttgc cttgtgtcat cagggtatgc gcaatcccta acatcagtgc cacgtcggtg
    20881 cccatattcg gcgcgatcca ggtggcatta tcgccaaaga attcgatggt ttcggagcgg
    20941 ataggatcaa tggcaatcac tggtttgcca gattttttca gctgatggaa gtattccagc
    21001 ccttgctcat cggtactgct ccaggcaatt tttaaggtat tcagcgggtt cattccccac
    21061 agcaccacaa cctggctgtt ttccagaatc agcggccagg aggcctgctg ttcatacacc
    21121 tctacagaac cgaccacatg cggcatgatc acctgtgctg ccccggttga ataatcgccg
    21181 ctatgcccgg aataaccgcc cgccaggttc atataacgtt gcagtaaggt ttgcgcttta
    21241 tgcaacacgc cagaagagcg ccagccgtaa gatccggcaa aaatggccga tggtccgtta
    21301 gctttacgaa tacgctcatg ttgctcatga atcagtttta atgcctgttc ccagctcacc
    21361 tgtacatagg tatcttcgcc acgacctttc gccggttgca gtggattgtc gagatagctt
    21421 tttctcacca tcggatgctg aatacgcgcc gtggtgtgta cctgatccgc cgccgtagac
    21481 tgtaaggagt tcggtatggt tttcgccagc gcgcctgtcg aagaaacaat cttgccgtcc
    21541 ttcacttcta cgctcatcgc tccccaacgt cccgcggtga ggattttacc gcccttctct
    21601 tctgcccatg cggggagcgg tgctgccgat gtcaccacca gcgctccagc ggcaataccg
    21661 ctgtgtttaa taaattcacg tattgttaat gccataactt cctccctgat caacgaggat
    21721 cactgtttct cggtaatatc tttggcgttg tactggaaat accgcgttaa aatgtccagt
    21781 tcgttttcgc tcatgctggt tcgtgccccc attcctttgg caatggacgg ccacgcattg
    21841 acggtgtaat ggtcggcggc aatagaggca tgacaaccag cgcaataggt atcgtcaagt
    21901 ttttcagcgt attgccatag cggtttacgg tccgctaatg cgggatcggt aagcgcaccc
    21961 tgtaaagacg cctgacgcca ttgattgccg tattcgtcag cctgccattc cccgtttacc
    22021 gtgagcgcct tgataccttc ttcacttaat gtggctagcg ccagccgttg acctgccgcc
    22081 aggtagagcg tgttttcact gccctgcatt tgataaccct gcaacagaac gatcggctgt
    22141 ttgccactgg catcaacgac ggtgagatcg gtaccaggat tcacggtagc cagctcgcct
    22201 atgcgagaag ttttgaaagg ataaatatgt gcgccattag tcactgaagt agcggcctga
    22261 ctttccagct catgcgccgc gttgtcatcc atttttattt ctggcggaaa atgggcaatg
    22321 cctttatgac aatcgataca ggtttcgccg cccttttgtg ctttgttatg cattttctgc
    22381 gcagattcac tttgcgaggc aatatccatg gcatcaaaag aatggcaact acggcacgtt
    22441 gcagagtcag ttgcttttaa ttctttccat actgtttcgg ccatttcctg gcgatgagtt
    22501 tcgaacttat cgtcactgtc tattttgccg ctaacaaatt catgataaat atctttagat
    22561 gcttttaatt tagcaaataa ataatccatc cctgactttg gaatatggca atcggcacat
    22621 tccgcacgta tccctttctg gttcgaaaag tggacagttc cctgatattc ctcaaaaggt
    22681 ttactcatcg agtggcagga aaggcaaaat gctgtatccg acgttttatg taaggctttt
    22741 tgcgccagca ataacccgcc gccaccaacc acaaccgcta tcagcaaaaa caataaccca
    22801 atgcgttttt tccctcgcat agattcctct aaataaattc cagaacaccg aaatcattgt
    22861 ataattaaat acacaacgaa ttaaccgctt gcataactcg gcacaacact gcctgaaata
    22921 atcgataaag aatatgattt attacaatgt aatcattaat tgctaaggaa taacccagtt
    22981 gctatttaga atatatattt atcagtttta gcaatttaaa tcccataatc aatgtgaata
    23041 taaaaaatat ttttagcaca acgctatgcg cactgtcacc cgctcaacag atcacggtta
    23101 tcgttcgttt tttatactgc tcagggataa aaaaaggccc ctgttgaaat tgcaggggcc
    23161 tggtacgagc aagcatcata ttgggcgaca tgatgcaacg gtaaaaatca tttggcctga
    23221 tggcgttcaa tgattccttt catttcagca accgccgccc cgtctacgat ataacgcgaa
    23281 tactcgtccg cgtgttcatc tgatgacatg gacaatcctt gattacgata acgcatcggt
    23341 gaggcttgcc acgctcccgc ggagctatgg acttccagca ctccggcatc gaggaagtgg
    23401 tgcaggtttt ctgcacggac tcctgctcca gccataatga ttggagcatc acgatgggca
    23461 ataagttcca taatttttga taaaccttgc agtgcgtctg atttttgccc tgatgtcagt
    23521 acccgggcaa tgcccaattc cgcaagatta ttgagtgtat ataaagggtt agcgcacata
    23581 tcgaaggcgc gatgaaaagt cactgccagc ggaccggcag cagccattat tttttccatt
    23641 cgtggcatat cgacattccc atcaacatcg agaacgcccg tcaccagtcc aggaaaacct
    23701 aattcgcgga ccgtgcgcac atcctcaaga atggcggcaa actcaccgtc gctgtagcaa
    23761 aaatcaccac cgcgtgggcg aattatcgga tgcacaggga tcgtcacccg ctggcgcacg
    23821 gatttcagtc cccccaacga cggcgttaag cccccctctt ttggggctgc gcataattca
    23881 actctgtctg cgccgttttg ctgcgccgtt agtgcacatt ccatgctgta acagcaaatt
    23941 tccagtaatg ccatttttgc tccttaatta cgctgactgc tcgctggcaa cgatctcttc
    24001 aatggaccac ggatgaaact taatagttgt tttgccatcg gtgaccgcca gcgtcggatt
    24061 cggtaatcgc tcatgctcac cttttggtga actggtttta actgagatcc ccggtaggct
    24121 gagtccttca tcagaaagca atgccagcgc acgggcattc agcgtttctg gatcacccgg
    24181 cagaacgatt tcaatatgtt cccaaccttc gtgtgggtaa cgtttttccc caggccacgg
    24241 tagctccaca ttggaaaact gccagtgcgc aacctgtacc ggttcatgca atttaaacag
    24301 acaaatcggt ctgccattga tcattttttc tgacaaaagc tcgccacact gttcaaaacc
    24361 gcgacgccag cgctcagcgg tgacgttttg atggcaacgc aaagaaatgt gatcggcagt
    24421 cagcggagtg atatccaacc ccagacggcg ggaaagttca tctaatgcgt gggtaaatcg
    24481 cggtaaatcc gatgcaatat cctgcagctc gtcgatagat tgccagttcg ccataatcac
    24541 tcttcgtctt tcagtaaaag cgttaattta ccctgttgcc ctgtgccaac caaccgctga
    24601 tttcacgccg cttctgatgc aatagtgaaa acggcaatac gccacgcgca cgttgctgac
    24661 gaaaacagcc atttgcagta tactcccacc ctaatttctt taactggtgc gggcaatttt
    24721 tgctcgcttc atcaatgtaa ggtattccgg tgaatattca ggctcttctc tcagaaaaag
    24781 tccgtcaggc catgattgcg gcaggcgcgc ctgcggattg cgaaccgcag attcgtcagt
    24841 cagcaaaagt tcagttcggc gactatcagg ctaacggcat gatggcagtt gctaaaaaac
    24901 tgggtatggc accgcgacaa ttagcagagc aggtgctgac tcatctggat cttaacggta
    24961 tcgccagcaa agttgagatc gccggtccag gctttatcaa cattttcctt gatccggcat
    25021 tcctggctga acatgttcag caggcgctgg cgtccgatcg tctcggtgtt gctacgccag
    25081 aaaaacagac cattgtggtt gactactctg cgccaaacgt ggcgaaagag atgcacgtcg
    25141 gtcacctgcg ctctaccatt attggtgacg cagcagtgcg tagtctggag ttcctcggtc
    25201 acaaagtgat tcgcgcaaac cacgtcggcg actggggcac tcagttcggt atgctgattg
    25261 catggctgga aaagcagcag caggaaaacg ccggtgaaat ggagctggct gaccttgaag
    25321 gtttctaccg cgatgcgaaa aagcattacg acgaagatga agagtttgcc gagcgtgcac
    25381 gtaactacgt ggtaaaactg caaagcggtg acgaatattt ccgcgagatg tggcgcaaac
    25441 tggtcgacat caccatgacg cagaaccaaa tcacttacga tcgtctcaac gtgacgctga
    25501 cccgtgatga cgtgatgggc gaaagcctct acaacccaat gctgccagga attgtggcgg
    25561 atctcaaagc caaaggtctg gcagtagaaa gcgaaggggc gaccgtcgta ttccttgatg
    25621 agtttaaaaa caaggaaggc gaaccgatgg gcgtgatcat tcagaagaaa gatggcggct
    25681 atctctacac caccactgat atcgcctgtg cgaaatatcg ttatgaaaca ctgcatgccg
    25741 atcgcgtgct gtattacatc gactcccgtc agcatcaaca cctgatgcag gcatgggcga
    25801 tcgtccgtaa agcaggctat gtaccggaat ccgtaccgct ggaacaccac atgttcggca
    25861 tgatgctggg taaagacggc aaaccgttca aaacccgcgc gggtggtaca gtgaaactgg
    25921 cagacctgct ggatgaagcc ctggagcgtg cacgtcgtct ggtggcagaa aagaacccgg
    25981 atatgccagc tgacgagctg gaaaaactgg ctaacgcggt tggtattggt gcggtgaaat
    26041 atgcggatct ctccaaaaac cgcaccacgg actacatctt cgactgggac aacatgctgg
    26101 cgtttgaggg taataccgcg ccatacatgc agtatgctta cacgcgtgta ttgtccgtgt
    26161 tccgtaaagc agaaattaac gaagagcaac tggctgcagc tccggtaatc atccgtgaag
    26221 atcgtgaagc gcaactggca gctcgcctgc tgcagtttga agaaaccctc accgtggttg
    26281 cccgtgaagg cacgccgcat gtaatgtgtg cttacctgta cgatctggcc ggtctgttct
    26341 ctggcttcta cgagcactgc ccgatcctca gcgcagaaaa cgaagaagtg cgtaacagcc
    26401 gtctgaaact ggcacaactg acagcgaaga cgctgaagct gggtctggat acgctgggta
    26461 ttgagactgt agagcgtatg taatcgattt ttcgtgagag tgaagcctga tcagggttag
    26521 ccgatcaggc ttttttattg ccatctaaat gtattctgaa aacggacatg ccattgtttt
    26581 ctcactgttg agtaagaggc cagaagcgta atatccaggc ccaggaaaac gataactgtt
    26641 caatttaagg aataccgaag tgtttaaatt tcttgtatta acattaggca ttatctcttg
    26701 ccaggcttac gcagaagata cggttatagt aaacgaccat gacacttcag ccatcaaaga
    26761 ttgttggcaa aaaaattcag atgatgatac tgacattaac gtgatcaaat catgcctgcg
    26821 acaagaatac aatctcgtcg atacgcaatt aaataaagcc tatggtgaag cttatcgtta
    26881 tatagaacaa gtgccacgca caggtgcaaa aaaacttgat actgaacaac ttaacttgct
    26941 taaaa
//
LOCUS       DAJTYG010000051        26412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTYG010000051 DAJTYG010000000
VERSION     DAJTYG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.051"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            117..1088
                     /locus_tag="OO568_002286"
     CDS             117..1088
                     /locus_tag="OO568_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6641624.1"
                     /translation="MRTTRNLLLMATLLGSALFAMAADKEMTIGAIYLDTQGYYAGVR
                     QGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSSRTVR
                     RASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIDQPKI
                     AVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIISTPDLN
                     AIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDISGKKM
                     GNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            1111..2622
                     /locus_tag="OO568_002287"
     CDS             1111..2622
                     /locus_tag="OO568_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6641625.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2647..3645
                     /locus_tag="OO568_002288"
     CDS             2647..3645
                     /locus_tag="OO568_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6641626.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNAQPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3678..4772
                     /locus_tag="OO568_002289"
     CDS             3678..4772
                     /locus_tag="OO568_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6641627.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGAVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4784..5656
                     /locus_tag="OO568_002290"
     CDS             4784..5656
                     /locus_tag="OO568_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6641628.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALALG
                     ESTTSEMSLRVEWL"
     gene            complement(5704..6126)
                     /locus_tag="OO568_002291"
     CDS             complement(5704..6126)
                     /locus_tag="OO568_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6641629.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6223..7430)
                     /locus_tag="OO568_002292"
                     /pseudo
     CDS             complement(6223..7430)
                     /locus_tag="OO568_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(7440..8252)
                     /gene="hcaB"
                     /locus_tag="OO568_002293"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(7440..8252)
                     /gene="hcaB"
                     /locus_tag="OO568_002293"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6641630.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVAALELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(8249..8569)
                     /locus_tag="OO568_002294"
     CDS             complement(8249..8569)
                     /locus_tag="OO568_002294"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6641631.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(8569..9087)
                     /locus_tag="OO568_002295"
                     /pseudo
     CDS             complement(8569..9087)
                     /locus_tag="OO568_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
     gene            complement(9084..10445)
                     /locus_tag="OO568_002296"
     CDS             complement(9084..10445)
                     /locus_tag="OO568_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6641632.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            10581..11471
                     /gene="hcaR"
                     /locus_tag="OO568_002297"
     CDS             10581..11471
                     /gene="hcaR"
                     /locus_tag="OO568_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR6641633.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            11631..12770
                     /locus_tag="OO568_002298"
     CDS             11631..12770
                     /locus_tag="OO568_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6641634.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12762..14042)
                     /gene="csiE"
                     /locus_tag="OO568_002299"
     CDS             complement(12762..14042)
                     /gene="csiE"
                     /locus_tag="OO568_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6641635.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKHFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(14233..15087)
                     /locus_tag="OO568_002300"
     CDS             complement(14233..15087)
                     /locus_tag="OO568_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6641636.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVLRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTSPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(15232..16035)
                     /gene="suhB"
                     /locus_tag="OO568_002301"
     CDS             complement(15232..16035)
                     /gene="suhB"
                     /locus_tag="OO568_002301"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6641637.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            16154..16894
                     /gene="trmJ"
                     /locus_tag="OO568_002302"
     CDS             16154..16894
                     /gene="trmJ"
                     /locus_tag="OO568_002302"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6641638.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            17164..17652
                     /gene="iscR"
                     /locus_tag="OO568_002303"
     CDS             17164..17652
                     /gene="iscR"
                     /locus_tag="OO568_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6641639.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            17764..18978
                     /gene="iscS"
                     /locus_tag="OO568_002304"
     CDS             17764..18978
                     /gene="iscS"
                     /locus_tag="OO568_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6641640.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            19006..19392
                     /gene="iscU"
                     /locus_tag="OO568_002305"
     CDS             19006..19392
                     /gene="iscU"
                     /locus_tag="OO568_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6641641.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            19409..19732
                     /gene="iscA"
                     /locus_tag="OO568_002306"
     CDS             19409..19732
                     /gene="iscA"
                     /locus_tag="OO568_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6641642.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19828..20343
                     /gene="hscB"
                     /locus_tag="OO568_002307"
     CDS             19828..20343
                     /gene="hscB"
                     /locus_tag="OO568_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6641643.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            20360..22210
                     /gene="hscA"
                     /locus_tag="OO568_002308"
     CDS             20360..22210
                     /gene="hscA"
                     /locus_tag="OO568_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6641644.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMDKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            22212..22547
                     /gene="fdx"
                     /locus_tag="OO568_002309"
     CDS             22212..22547
                     /gene="fdx"
                     /locus_tag="OO568_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6641645.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            22559..22759
                     /gene="iscX"
                     /locus_tag="OO568_002310"
     CDS             22559..22759
                     /gene="iscX"
                     /locus_tag="OO568_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6641646.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22818..24101
                     /gene="pepB"
                     /locus_tag="OO568_002311"
     CDS             22818..24101
                     /gene="pepB"
                     /locus_tag="OO568_002311"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6641647.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            24243..25019
                     /gene="sseB"
                     /locus_tag="OO568_002312"
     CDS             24243..25019
                     /gene="sseB"
                     /locus_tag="OO568_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6641648.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25840..26322)
                     /gene="sseA"
                     /locus_tag="OO568_002313"
                     /pseudo
     CDS             complement(25840..26322)
                     /gene="sseA"
                     /locus_tag="OO568_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT         6258 a         6582 c         7310 g         6262 t
ORIGIN      
        1 gttcactaaa ccctattaac tgtaatattt gagcggcacg cgctgccgct catcacaaaa
       61 taattagaat aatccgttgt taataaaaac cctacaggag gcactatgcc taaaaaatga
      121 gaacaacccg taatctatta ttaatggcta cgctattagg tagcgcacta tttgccatgg
      181 ctgcggataa agaaatgacc attggtgcaa tataccttga tacccaggga tattacgctg
      241 gagtgcgtca gggcgttcag gatgcggcaa aagattcttc agtacaggta cagttaattg
      301 aaactaacgc ccagggggat atttcgaaag aaagtacctt tgttgatacc ctcgtggcgc
      361 gtaatgtcga tgccattatt ttatcggcag tgtctgaaaa tggcagtagc cgtaccgttc
      421 gtcgcgccag tgaagcgggt attccggtga tttgctacaa cacctgtatt aatcaaaagg
      481 gtgtcgataa atatgtctcg gcgtatctgg tcggcgatcc actggaattt ggtaaaaaac
      541 tgggtaacgc tgccgccgat tattttattg ccaataaaat tgaccagccg aaaattgccg
      601 tcatcaattg cgaagccttt gaagtttgtg tgcagcgacg taaaggattt gaagaagtat
      661 taaaatcccg cgttcccggc gcgcaaattg tcgctaacca ggaagggact gttttagata
      721 aagcgatttc cgttggtgaa aaactgatta tctccacgcc ggatctcaac gccattatgg
      781 gggagtcggg cggtgcgaca ctcggcgcgg taaaagcggt acgtaatcaa aatcaggccg
      841 gaaaaattgc tgttttcggt tcggatatga caaccgaaat tgctcaggag ctggaaaaca
      901 atcaggtgct gaaagcggta gtggatattt ccggtaagaa aatgggcaat gctgttttcg
      961 cgcaaacatt gaaggttatc aataaacaag ccgacggtga aaaagtgatt caggtgccaa
     1021 tcgatctcta taccaaaacg gaagatggta aacagtggct ggcaacgcac gttgatggtc
     1081 tgccctaatc gctgattcgc agaggtccct atgttcacgg caacagaggc agtcccggta
     1141 gcaaaagtgg tggcaggaaa taagcgttac cccggcgtcg ttgcgctgga taacgttaac
     1201 ttcacgctca ataaaggcga agttcgtgcg ctgttaggta aaaacggcgc gggcaaatcg
     1261 actctcattc gaatgcttac cggcagcgaa cgcccggata gcggtgatat ctggattggc
     1321 gagacgcgac tggaaggtga cgaagctacg ttgactcgcc gtgccgctga actgggggtt
     1381 cgtgcggttt atcaggaatt aagtctggtg gaagggctga cggtggcaga aaacctctgc
     1441 cttggtcaat ggcctcgccg caacggcatg attgattacc tgcaaatggc gcaggatgcc
     1501 caacgttgct tacaggcgct gggcgttgac gttagtcctg aacaacttgt ttcaacgcta
     1561 agcccggcgc aaaagcaact ggtggaaatt gcacgggtga tgaagggcga gccgcgcgtg
     1621 gtcattcttg atgaacccac cagctcgctt gccagtgcag aagttgaact ggtgatcagt
     1681 gcggtgaaaa agatgtcggc actgggcgtg gcggtgattt atgtcagcca ccggatggaa
     1741 gaaatccgcc gcattgcctc ctgtgccacc gttatgcgcg atggtcaggt ggcgggcgat
     1801 gtgatgctcg aaaacacctc cacgcatcat attgtgtcgc taatgctagg gcgtgatcac
     1861 gttgatattg cgccggttgc ccctcaggaa attgtggatc aggccgtact ggaagtccgt
     1921 gcgttacgcc ataagcccaa gctggaggat atcagcttta cgctacgtcg tggcgaagtg
     1981 cttggcattg ctggcctgct gggggcaggg cgcagtgaat tgctgaaggc gattgttggg
     2041 ctggagacgt atgaacaggg cgaaattgtt atcaacggcg agaaaatcat gcgccccgat
     2101 tacggcgaca tgctgaaacg cggcattggc tatacgccag aaaaccgcaa agaagcgggg
     2161 atcattccct ggctgggcgt tgacgaaaat acagtgctga ccaatcggca aaaaatcagc
     2221 accaacggtg tgctgcaatg gtccaccatc cgccgcctga ccgaagaggt gatgcagcgg
     2281 atgacggtca aggctgccag tagcgaaaca cccatcggca cgctttctgg tggcaatcag
     2341 caaaaagtgg tgatcggtcg ttgggtctat gccgccagcc agattttgtt gctcgacgag
     2401 ccaacgcgcg gcgtcgatat cgaagccaaa cagcagattt accgtattgt ccgtgagctg
     2461 gctgccgaag gaaaaagcgt ggtgtttatc tccagtgaag tggaggaatt gccgctggtg
     2521 tgtgaccgca tcctgttatt acagcacggc acgttctcgc aggagtttca ctcgccggtc
     2581 aatgtggatg agctgatgtc cgccattctg tctgtgcact gatgaatttt accgaggaac
     2641 ctgaagatgt ctgcttcgtc attaccattg ccgcagggca agagcgtctc gctcaaacaa
     2701 tttgtcagtc gccatattaa tgagatcggt ttgctggtgg tgattgccat tctttatctg
     2761 gtcttctccc tgaacgcgcc tggctttatc tcattgaata accagatgaa cgtgctgcgc
     2821 gatgccgcca ccattgggat tgccgcctgg gcgatgacgc tgattattat ctccggtgaa
     2881 attgatgtca gcgttgggcc gatggtggct tttgtctcgg tgtgcctggc atttttgctg
     2941 caatttgaag ttccgttggc gattgcttgt ctgctggtgt tgctgttagg cgcgctgatg
     3001 ggaacgctcg ccggggtgct gcgcggcgtg tttaacgtac caagtttcgt tgccacactg
     3061 gggctgtgga gcgctctgcg cggaatgggg ctgtttatga ccaacgcaca gccagtgccg
     3121 attgacgaaa acgaggtgct ggactggctg ggcggacaat ttctcggtgt gccagtatcc
     3181 gcgctgatca tgatggtgtt gtttgcgctg tttgtgttca ttagccgaaa aaccgccttc
     3241 gggcgctcgg tttttgccgt tggcggtaat gccacggcgg cgcagttgtg cggtattaac
     3301 gttcgtcgag tgcgcattct tatctttacc ctttcgggat tattagcggc ggtgaccggc
     3361 attttgttgg cggcacgcct cggttccggt aacgcaggtg ccgcaaacgg tctggagttt
     3421 gacgtcatcg ccgcggtcgt ggtcggcggt acggcacttt ccggcggtcg cggttccctg
     3481 ttcggtacat tgcttggtgt gctggtgatt acgctaatcg gtaacggtct ggtgctgctc
     3541 ggtattaact cctttttcca gcaggtggta cgcggcgtca tcatcgtggt ggcggtgctg
     3601 gcgaatatct tgctgaccca gcgaagcagt aaagcgaaac gctaaacctt aacgcccctg
     3661 gcgcgggtgc ggcagggatg aaaattaatc gtattgttaa tgagggattt atgaaaacga
     3721 tgctggcagc ttatttacta ggaaattcaa ccgtcgatct gcgggaagtt gcggtgccga
     3781 cgccggggat taaccaggta ctgatcaaaa tgaaatcctc cgggatttgc ggaagcgatg
     3841 tccactatat ctatcatcaa caccgtgcca cggcggcggc acccgataaa ccgttatacc
     3901 agggatttat caacggtcat gaaccgtgcg ggcagattgt ggcgatgggg caaggctgcc
     3961 gccattttaa agagggcgac cgcgtgctgg tgtatcacat ttctggctgt ggtttttgcc
     4021 cgaactgccg ccgcggcttt cctatctctt gtactggcaa aggaaaagcg gcttacggct
     4081 ggcagcgtga cggcggtcat gccgaatatc tgctggcgga agaaaaagat ctgatcctcc
     4141 tgccggatgc gctgagctac gaagatggtg cgtttatcag ttgcggcgtt ggcacggcct
     4201 atgaagggat tttgcgcggc gaagtttccg gcagcgataa cgtgctggtg gtcggtctgg
     4261 gggcggtcgg catgatggcg atgatgctgg cgaaaggtcg cggtgcaaaa cggatcatcg
     4321 gcgttgatat gctgccggaa cgtctggcga tggcaaaaca gttagggggg atggatcacg
     4381 gctatttagc aaccaccgaa ggtctgccgc agataattgc tgaacttacc cacggtggcg
     4441 cggatgttgc gctcgattgt tccggtaatg ccgcaggtcg cttgctggca ctgcaatcca
     4501 ctgctgactg ggggcgggtg gtttacattg gtgaaaccgg aaaagtggaa ttcgaggtca
     4561 gcgctgatct gatgcaccat caacggcgga ttatcggctc ctgggtgacc agtctgttcc
     4621 atatggaaaa atgcgcccac gatttgactg actggaagct gtggccgcgt aacgccatta
     4681 cccatcgctt ctcgctagaa caggcaggtg atgcctatgt gctgatggcg agcggcaaat
     4741 gcgggaaagt tgtgattaac ttcccggatt aaggagttag cggatgacca tctatacctt
     4801 atcccacggg tcgctaaagc tggacgtttc tgaccagggc ggcgtaatcg aaggcttctg
     4861 gcgcgatacg acgccgctct tacgtcctgg taaaaaaagc ggtgtggcaa ccgatgcctc
     4921 atgttttccg ctggtgccat ttgcaaaccg ggtgagcggc aaccgttttg tctggcaggg
     4981 gcgtgagtat caactgcaac cgaatgttga gtgggatgcg cactatctac acggcgatgg
     5041 ctggctgggc gagtggcaat gtgtctcgca cagcgatgat agtttgtgtc tggtgtatga
     5101 acatcgcagc ggtgtctatc actatcgggt aagtcaggcg tttcatttaa cggcggatac
     5161 gctgacggtg acgctctctg tcaccaatca aggggcagag acgctgccat ttggtaccgg
     5221 ctggcatcct tattttccgt tgtcaccaca aacgcggatt caggcgcagg cgagcggtta
     5281 ctggctggaa cgggagcagt ggctggcggg tgagttttgc gagcagctac cgcaggaact
     5341 ggattttaac cagctcgcgc cgttgccgcg tcagtgggtg aacaatggtt ttgccggatg
     5401 gaatggtcag gcccgcatcg agcagccgca ggaaggttat gccatcatca tggaaacgac
     5461 gccacctgca ccgtgttatt tcatctttgt ttccgaccct gcgtttgata aaggatatgc
     5521 gtttgatttc ttctgtctgg aaccgatgag ccatgcgccg gatgatcatc atcgcccgga
     5581 agggggtgat ctcattgcgc tagcactagg ggaatcaaca acctcagaga tgtcgttgcg
     5641 ggtagagtgg ctgtaattta taaaatgccg gataaggcgt tcacgccgca tccggcatct
     5701 ttcctaacgc cgatcgagag aaattgcccc cggtccggta attgccaata gcaagaacgc
     5761 gccagcgata ctgacattct tccagaaatt aatcatattt ggcccaaccg catcgccggt
     5821 catatcccag taatggtgac caatcaccgc cgtacccagc gtgtagaaaa taaacagcac
     5881 cgccagcgga cgggtgaaaa agccaagcac gattaatatc gcggcgggca cttccataac
     5941 taccgcaata atcgctgcca gcatcggcat tggcgcgccc aacgaggcca tatattggac
     6001 cgtaccgtca aagcccatca ttttgggaaa accaaaaata atgaaaatta agaccacggc
     6061 gatacgggca attaataaca aaacggggcg ggcagctcca aaatcaaaat aacgtagtgt
     6121 gttcataacc aatccctttg caagataatg tgatttaaac gtaatacaca ctcttgcacc
     6181 tcgccacgcc ccaggcattg agatattcgc taattatcct ggttacagtg atttaagcgc
     6241 gatgttctca tctatcagca gtttcgcatc aaacgttttg ccgctctgga tccatttgcg
     6301 aattgggcga atctcacgcc cctgattcag cgtgaccgca ccgataagca cgccgttttg
     6361 cagattaaac caaatcgcct tctgagtttc cgggttgcca cgacaaagcc agtcatcgcc
     6421 acgcatatcg ccaataaact gtaagttatc actgtactga tcgctccaga accacggcgg
     6481 cggcagtagc ggtagcggta gccctaacat tgcggcagcg gcaatttgcg cctggttatt
     6541 ggcgttttcc cagctttcgc agcggtgtag tgcaccatta tcaagacgag tgattgccac
     6601 atcgccaccg gcaaagatcg cgggatcgca ggtgcggcaa gcctcatcaa tgacaatgcc
     6661 attggcagta tcaaggttgg cctcgcgagc cagttgctcg ttggcgctga tactaatacc
     6721 gtaaatcacc acatcagcct gaagcgtttc cccactttgc agcgtcagtt ctactttttc
     6781 accatcgacc acatgttcaa tggcattatt gagcagaatg cgcacaccag cctgctgatg
     6841 gcgctgtaaa agatagcgtt gcacgggcgg tggtgcatta cggcccatga cggttgccgc
     6901 cagttcaatc actgtcacct tacatctgcg ctgcgctgcg tggcgctggc agccagttcc
     6961 agaccaatag ttccggcacc gacaatcacg actgaccgtt cgggctgcag aacttctcgc
     7021 agtctggcgg catcaccggc atggtgtagg gtaaagcagc gttctcccag tgcatcaagc
     7081 aacggcagcg gtcgagctgc cgcgccggtt gctataaaaa gctgatccca gtgccagctt
     7141 tcgccgttgg ttaacactaa ctctcgtgtg tcgcgaccca gcgttttgat ggttacaccg
     7201 gaatgcagat gaacattgtt ttcctgccac cagttagcgg gtaacacctg ctgtaactgc
     7261 ggggaatctt ccagcaacat ggatttcgag agtggaggtc gttcataagg aagatgtcgc
     7321 tcatcggaaa acagatgcag ctcaccggtg aacccttgct ggcgtagcga ggccgcagcc
     7381 attgccgccg cttgcccgcc accgacaata atgatcgttt tttctttcat cgtgcttcct
     7441 taaagatcca gcccagccgc tacgtggcga atgccgcgaa tcgctaaacc cgcatcagcg
     7501 ttgatcatca caccgcttaa tgcgcgatta ttgcgccgcg atgccaacat cacatacggc
     7561 cccgtaaaat ccgccggttg cgggaaaaat tgcagcggta aaatggcggc aattttctcc
     7621 ggcgtcagag actgcattat cgaggtttca ctttgcccga gcgcctgtgg gccgcgcagg
     7681 tcgctggcca taccacacgg gccgacgcca ttcacccgca ctttcggtgc cagttcataa
     7741 gccagttggc gaataagtcc ggttgcggca tgtttactgg tggtgtacag cgggccaccg
     7801 ccgccaggat accaggcggc atttgacagt gtgaaaatca tgctgccttc actggcgatt
     7861 aacgccggag cgcaggcttt tgcgcccagc aggtaaccga gtacgttgac gttaaacagc
     7921 tcgtggaagc cggtttcgag cgtctctgcg ggagtattaa ccagtgaggc attgtgatcc
     7981 cagatgcctg cattgccgat aaaacaatcc agcttgccgg aacgagtcag gatctgatcg
     8041 accgcgcgtt gataatcggc ataacaggtc acgttacctt ccaccgccag aatatgttcg
     8101 ccaaatcgct gacgcagact ggcgactttt gccgccgaca gttccagcgc ggcaacctgc
     8161 gcgccttctt cgataaatcg ctcgaccagc gccagcccta atcccgatcc gccgccggta
     8221 ataaaaatgg actcgttatg cagatcgctc atggctgcgc ctccggtaag tcgatgaaaa
     8281 tgtcaccacc ttcaacgtgt actggataag tggtgagcgg atcggtggcg ggcaggcata
     8341 acgctttccc cgttttcagg caaaaactgg cggcgtgtag cgggcactcc accgtggcgt
     8401 catcttccag atacccttct gacatcgacg cattaccatg gctgcaacga tcgttaatgg
     8461 cataaaactc gccgcccacg ttgaacaagg cgatgacggg cgaggtatct atccggagag
     8521 cctcaccctc cggcacatcc gcaacgggac acgcataaat tcgattcatc agaacagtac
     8581 actcaggtta tgggaagtga tcaccgcttg atccaggacg atatcccgtt ccagcaagcg
     8641 ccagttgtca tcttccagac ggcgaacttt gtcgaaacgc gttccaacat agaatgtttc
     8701 atcgcgctct ttttgtgccc gataaagtag ataattcacc ctcacggcaa atacgtttgg
     8761 gatgtcggtt tcgcttacct ggcagttgct gattaagtga cgagtgcgtg acggcggctc
     8821 ttctgcctag gccatgcccg tttccagacg ggcgattcgc cgctccagct ggtctttggt
     8881 gtcattgaaa atccaggtcg tcggcggctg gacgcctttg cgacggtcgc gtgtttgcgc
     8941 gttaaccgtg gtgcgcatgg tgtaacgaat ctcttcgtcg agctgtgcca accagtcacg
     9001 aaatttccag tcatccagca gactggcttc gtgaaagaga aactggctaa tgcggtgatg
     9061 taactccagt gaaacttgcg cactcatttc atcacctcct gctggtaagc ggcggtttta
     9121 tcgagcactt cctgccagct ttcgctactc agaagatcgg cccagcgttg gtacattcca
     9181 cgagcggcag tttctgaaaa gatatagtta gtaatgccag gaatgccgtc gtcgcggcgc
     9241 ttttcctgac caagccccat ttccagacac agtttgctgt tgcgggcgcg gtggccttta
     9301 agcaatttct ggatttcaca ccagttctcc gagtcatcct gctcgagaaa accagcagga
     9361 ccaaaagcac gagtggcgct gttttcaaaa gcggctttaa cttcatcgga ggcggctttg
     9421 tcagtaatac agaacgccca cacttcaact tgatcagggc cgcgcggatg ccagacgcgg
     9481 agtgtggcag tgccgtttag ccatgaaagc gtggggaata tattgttatg acccgccagg
     9541 cgcagggcgc gaacttcacc taaacgttgt tctgcttcgg cataggtttc gcggtaatag
     9601 cttgaaactg cgccatcgac ccagacatta gcatccggtt tttcagtaaa gaagaaaccg
     9661 ctaccgtgac cgtcctgacc aaattgcagc gcatctttgg cggtttccca caccgggcgg
     9721 gcggtttgtc catcaccgag gcgcttatcg ctgccatcat ctttcgcccc taatacctga
     9781 acggcagaag catggctgaa cagagcatga tactggtcac tggcgaactg ctctgccggg
     9841 aatttccagt tacagttgat cacccacttt tgtacgccgc cgacaatttc ggtgccgcct
     9901 tcgcgacgat ccagcatgcc atccagatac caggcaatgt cacccaggta atcatgcagg
     9961 cccggtgcgc tggtatccca gttgccaaaa atcagtcctt tataactctc cacacaagga
    10021 acttcgttta gcccccagtg ggatttacac aacccttgtg ggtaggcgcg aggttccagc
    10081 ggtacatcga tcaactcgcc gttaatgcca taagaccagc cgtgatacgg gcaggtaaag
    10141 gcgcgagtgt tgccacaatc tgcataactc acacgcatgg cccggtggcg gcattggtta
    10201 agaaacgcct tgatgctgcc gtctttctgg cgcaccacga caactgcatc ttctcccatg
    10261 taggtgttaa agaaatcacc gggttttggg atctggcttt cgtgggcgag aaatagccag
    10321 caacgaccga aaatacgctc aagctccagt tggtaaatgt ccgggtcggt ataaatacgc
    10381 ggagtgaccc gaccattttg ggtgtcaatc agttggtaaa tgttcaaatc tgagggtgtg
    10441 gtcatatttt ttaatcctga aagataagat gtattgacct tctatttttg tgctataagg
    10501 atgttgctgg ttggttgcta atatgtgaaa tagttaattt ctaccagctg agacgttttt
    10561 tcgataacaa ggggaaggtg atggaactac ggcatttacg ctatttcgtc gcagtggcgc
    10621 aggcactgaa ctttacccgt gcggcggaaa aactgcatac ctcacagcct tcgttaagta
    10681 gccagatccg cgatcttgaa aactgtgtcg gtgttccgtt actggtgagg gataagcgca
    10741 aagtcgcgtt gacggcggcg ggagaatgtt ttctccagga cgcgctggca atcctcgaac
    10801 aagcggaaaa tgccaaatta cgggcgcgga aaattgttca ggaagacaga caattaacca
    10861 ttggctttgt gccatcggcg gaagtgaatt tattgccaaa agtattaccg atgtttcgtc
    10921 tcagacagcc agacaccttg attgagctgg tgagtttaat caccacgcaa caggaggaaa
    10981 aaattcgccg tggtgaactc gacgtcggct taatgcgcca tcctgtttat agcccggaga
    11041 ttgattatct ggagcttttt gacgaaccat tagtggtggt gttaccggtt gatcaccctt
    11101 tagcacatga aaaagagatc accgccgcgc aactggatgg cgtgaatttc gtcagtaccg
    11161 atccggcgta ttccggttcg cttgcgccga tcgttaaagc gtggtttgcg caagaaaaca
    11221 gccagccaaa tatcgtccag gtggcaacga atattctggt gaccatgaat ctggtgggca
    11281 tggggctggg cgtcactttg atacccggtt atatgaataa ttttaatacc gggcaggttg
    11341 tttttcgtcc cattgccgga aatgtgcctt ccattgcttt actgatggcg tggaagaaag
    11401 gggagatgaa gccggcatta cgcgatttca tcgccattgt gcaggaacgt ttggcaagcg
    11461 taacggcata attacgcatt atttttgcaa catgcgccgc ggatcgcagt tttttagtgc
    11521 ttgtgaactg gcgttacaga catcgggaag caggctcgaa tattcttatt tttcaacttg
    11581 caccaacatt cgattaaccc cagactaacg cctcctgacg ggagggactc atggttttgc
    11641 aatccacgcg ctggttggcg ctcggctatt tcacatactt ttttagttac ggcatttttt
    11701 tacctttctg gagcgtctgg cttaaaggga ttggtttaac gccagaaacc atcggcctgt
    11761 tattgggggc aggtctggtt gcccgttttc tcgggagttt gctcatcgcg ccccgcgtca
    11821 gcgatccttc ccgcctgatt tccgccttgc gcgtgctggc actgctgcca cttctctttg
    11881 ctgtcgcctt ctgggcgggg gcgcacgtag cgtggctgat gctggtgatg attggcttta
    11941 acctcttttt ctcaccgctg gtaccgttga ccgatgcact ggcgaatacg tggcaaaagc
    12001 agttcccgct tgattacggc aaagtgcgac tgtggggctc ggtggcgttt gtcattggct
    12061 cggcgctgac gggcaaactg gtcactatgt ttgattatcg ggtgatcctc gtgctgttga
    12121 cgttgggcgt ggcatccatg ctgctcggct ttctcatccg tccgacgatt cagccacaag
    12181 gggcaagccg ccagcaggag agcaccggtt ggtctgcgtg gttggcgctg gttcgccaga
    12241 actggcgctt tctggcctgc gtttgtttat tgcagggggc acatgcggcc tattacggtt
    12301 ttagcgccat ttactggcag gcagctggct actcggcctc ggcggtgggg tatttgtggt
    12361 cgctgggcgt ggtggcggaa gtcattatct ttgcgctgag taataaactt ttccgccgtt
    12421 gtagtgctcg cgatatgctg ttgatctcgg cgatttgcgg cgtagtgcgc tggggcatta
    12481 tgggagcaac tacggcgttg ccgtggttga tagtggtgca aattctgcat tgcggcacct
    12541 tcacggtctg ccacctggcc gccatgcgtt atattgctgc tcgccaggtt agcgaagtca
    12601 tccgtttaca ggcggtttac tctgccgtcg cgatgggcgg cagtatcgct atcatgaccg
    12661 ttttcgccgg tttcctgtat caatatctgg gccacggtgt gttctgggta atggcgctgg
    12721 tggcgcttcc ggcaatgttt ttgcgcccga aagttgttcc ctcatgctga ttcgagcatt
    12781 ttgcggatct gctcctgttg atgtgttgtc agcgtcaggt ccgtatagat cagcggtggt
    12841 gaaaagagcg gtaacggcat ggtataaggc gcaataatca gtgccgcgcc acgcggagcg
    12901 cctgtctgca aaaatgcctt taccgacata tgcttaatgt tgagcggcag taacgttagt
    12961 tcgcgtaact gctgctcaat ctgcgcttct cgctcgctat cattcccggt cagtagaata
    13021 atctgttttt catgcagatc gttttcctgc attagccagg caccgaaaat caccgcgacc
    13081 agaccacttt cctcatcaga aagatggacg ccgtattcac tttcaaatcc ggccagcgcc
    13141 gcgcgggtgg tgcgtaccag gcgtggatac agtctggcga actcttccgg cagggtatta
    13201 tcaataccga tggcaaacaa actgcgattt aacgcctggg cgaggtggat ataaagctga
    13261 tcacataacc cctgttcatc ataaaaacga acatttccca gctcacgaaa atggtttacc
    13321 agacgtttga tagactgacg caattgtctg tcccgctgat gcgcatcccg cagtggatcg
    13381 ggaacccgca acatggaaaa aagtaatgcc ataaacagtg gctcatcagg cggtacatgc
    13441 tggagcgccc gacgctgcca gtggcgtcca atttcctgcg ctacctgaaa ttcaaggcag
    13501 gattccgccc agcgacgttg gagcggatta aactgaggcg taattccagc gtggtgctgc
    13561 agcaaacaat attgcagata cagacacagg aaatgaatat cgcgcgattc aaagtgtttt
    13621 tgcagccgtc gggagcagag attcactaac gcttgtagat tggtatcgtc gtaaaagtta
    13681 cgcgcaattc cgcgccgctt cagttcactc ttcagggcgg gggtgaattg gctggtaata
    13741 aatgacggac acagacggaa accacgtcgt agccagtgaa ataaacacaa acgttggtta
    13801 agcactgtac cttcaacccg gtagctaccg tcataaccag tcgtcagtgt gagttgatga
    13861 tagcgcagga tctcccgtcc tgtttcgctg atatcaagac tggcaatatc atcatccaca
    13921 ccattaagct cgctgaaggt tgccatggtg gcggtcaacc ccggctgaaa gagcgtcagc
    13981 aagatctggc agcggcgctg gggagccgaa aggacagatg gtggagcaag cgtaggcatc
    14041 atctgtagat ctcccataag cataattgat aagaatagct aaagtttgct cagggaaatg
    14101 gcgaagggaa tgctaatcat cagaaatgtt ggcaagcgtc acagaatttt tctcatttta
    14161 aactgttctg accagtggat tagcatgtct taaggtggtt ttgtgaatga ttatcgtgct
    14221 agcgatcatg ggttagttct gcggattcaa cgcgctaagg ataaagtcca ccatctgttc
    14281 gcgataaaca ttgccgtgac gatcggaaaa agcatcaatg tgttcgccat ccgggattag
    14341 gatcaaccgt tttggctctt ttgccaggct atacaacttt tcgctgtgct gccatgggat
    14401 aacgtgatcg gctttaccgt gaatgagtaa aagcgggatc gggctgacgc tggcgatgta
    14461 attttcgccg ctgtaactct catcaagtaa gtagccactg ccggggatca tctggttggc
    14521 gatggttgca taagaggcaa atgtggagtc gaggatcacc gcacgtatgc cttcacgatc
    14581 accctgacca ataacagcaa gaatattcgc cccgccaata ctctgcccga acagcaccag
    14641 ccgttgtggg tttacatcac tgcgatggcg caccacattg atggcgcttt gcgtatcgtc
    14701 cagcaatccg gcctgggacg gagtgccttt tgatttacca aacccgcgat aatcaaacat
    14761 aaaaacgttg aaattacgct caggtaacca actgaccagc ggccagtggg cggacatatt
    14821 tccggcattg ccgtgagcat gaatgatggt tgcgatggcg ttgtcagcag ggctcgtcga
    14881 agaaggaata aaccagcctt gcagacgagt accgtcctta gccgtaaatt cgacggattc
    14941 cgccgaccag gggtcgggac cgtaaatttt atcgtcagga taatagaaga agttgatggc
    15001 aacgcgcagc actaaataaa cgcagaaggc aacaacaaag agaataaaca gaattttggg
    15061 aacgcgtttg ttcactggca gtgccataac ctttccttgc gacttcttta tcatccatta
    15121 atcggggaaa gatggtggga gagtgacgaa agaaagtaaa gcggtgagga agtatttcgg
    15181 aatagcgcct gaaaggcgag ggcgggtgag tgatatcacc cgcctgagtc attaacgctt
    15241 cagagcgtcg cttaactcgt cacgcatgtt cgccagtatg gctttcacaa cacgcgggtt
    15301 accagcaacg atgttaccgg tcagcatata gttatgacca ccggtgaagt cgctgacgat
    15361 gccgcccgct tcacgaacca gcagctcgcc tgccgcgaaa tcccacggac gcagaccgat
    15421 ttcaaagaaa ccgtcaacac gacccgcagc gacgtaagcc agatccagcg ccgcagaacc
    15481 ggtacgacgg aagtctgcac attcgttgaa cagcttgccg acgatgttga tgtaggtagt
    15541 ggcgtactgt tttgctttga acgggaagcc ggtcgccaga atggtaccgt cgagatcgcg
    15601 agcggtgcta ccgcgcagac ggtagccgtt cagctgtgcg ccctgaccgc gagtggcggt
    15661 gaacagttcg ttacgcatag gatcgtatac cacagcaact tcggtgcggc ctttgatacg
    15721 aacggcgata gataccgcga agtgcggcag acgtttgata aagttggtag taccatccag
    15781 tggatcgata acccattgaa catcctgatc agtaccttca agttcaccgc tttcttcggt
    15841 gatgatggtg tgctgtgggt aagatttacg aatcgtgtcg ataatcaccg cttcggcagc
    15901 tttatctacg ttggtcacga aatcgttact gcctttctgg ctcgcttcta cagcgtccgg
    15961 ggtttcatag tttttggcaa ttaaattacc cgccttgcgc gctgcgcgca cggcgatgtt
    16021 cagcatcgga tgcatcggtc tctctcactg gatgttaaag aacgggaaaa cggcgcagag
    16081 tatagcagcg agatcggcat ctgtcttcgt tttatgataa gatgcgtgaa taatctttag
    16141 atgaagaaag acaatgctgc aaaatattcg aattgtgctg gtggagacgt cacacaccgg
    16201 caatatgggt tctgttgccc gtgccatgaa aacaatggga ttaaccaatc tgtggctggt
    16261 taatccactg gtgaaacccg actcccaggc gattgccctg gctgcagggg ccagcgatgt
    16321 gattggtaat gctcacatcg tcgatacgct cgacgaagcg ttagccggtt gtagcctggt
    16381 ggtgggcacc agcgcacgct cccgcacgct gccgtggccg atgctcgacc cgcgcgaatg
    16441 cggtctgaaa agcgtcgctg aagcggcaaa taccccggtg gcgctggtgt ttggtcgtga
    16501 gcgcgtcggc ttgaccaatg aagagttgca gaaatgccat tatcatgtcg cgattgcggc
    16561 aaacccggaa tacagttcgc tgaacctggc aatggcggtt caggttattg cctatgaagt
    16621 gcgtatggcc tggctggcaa ctcaggaaaa tggcgagcag gtcgagcatg aagagacgcc
    16681 gtatccgctg gtcgatgatc tggagcgttt ttatggtcat ctggaacaaa cgctgctggc
    16741 aaccggtttt atccgtgaaa accatccggg gcaggtgatg aataaattgc gccgtctgtt
    16801 tacccgtgcg cgcccggaaa gccaggaatt gaatatcctg cgcgggattc tggcttctat
    16861 tgagcagcag aataaaggta acaaggccga ataacagtgg ttgcctgatg cgacgcttaa
    16921 cgcgtcttat caggcctaca atgtacagaa tcgtaggtcg gataaggtgt tcacaccgca
    16981 tccgacaaca ggtacaaacg ccacgataaa aaaatggcac tgaaggttaa atacccgact
    17041 aaatcagtca agtaaatagt tgaccaattt actcgggaat gtcagacttg accctgctat
    17101 gcaatacccc cacttttaca ataaaaaacc ccgggcaggg gcgagtttga ggtgaagtaa
    17161 gacatgagac tgacatctaa agggcgctat gccgtgaccg caatgcttga cgttgcgctc
    17221 aactctgaag cgggcccggt accgttggct gatatttccg aacgtcaggg aatttccctt
    17281 tcttatctgg aacaactgtt ttcccgtctg cgtaaaaatg gtctggtttc cagcgtacgt
    17341 ggaccaggcg gtggttatct gttaggcaaa gatgccagca gcatcgccgt tggcgaagtg
    17401 attagcgccg ttgacgaatc tgtagatgcc acccgttgtc agggtaaagg cggctgccag
    17461 ggcggcgata aatgcctgac ccacgcgctg tggcgtgatt tgagcgaccg tctcaccggt
    17521 tttctcaaca acattacttt aggcgaactg gttaataacc aggaagtgct ggatgtgtct
    17581 ggtcgtcagc atactcacga cgcgccacgc acccgcacac aagacgcgat cgacgttaag
    17641 ttacgcgctt aataaaaaga attcagaatc aggccggagt gctaaatact ccgtaaacac
    17701 ggtcgtacat ccagccggta gcctgattcc ttgcattgag tgatgtacgg agtttataga
    17761 gcaatgaaat taccgattta tctcgactac tccgcaacca cgccggtgga cccgcgtgtt
    17821 gccgagaaaa tgatgcagtt catgacgatg gacggaacct ttggtaaccc ggcctctcgt
    17881 tctcaccgtt tcggctggca ggctgaagaa gcggtagata tcgcccgtaa tcagattgcc
    17941 gatctggtcg gcgctgatcc gcgtgaaatc gtctttacct ctggtgcaac cgaatctgac
    18001 aacctggcga tcaaaggtgc agccaacttt tatcagaaaa aaggcaagca catcatcacc
    18061 agcaaaaccg aacacaaagc ggtactggat acttgccgtc agctggagcg cgaaggtttc
    18121 gaagtcacct acctggcacc gcagcgtaac ggtattatcg acctgaaaga actcgaagcg
    18181 gcgatgcgtg acgacaccat ccttgtttcc atcatgcacg taaataacga aatcggcgtg
    18241 gtgcaggata tcgcggctat cggcgaaatg tgccgtgctc gtggcattat ctatcacgtt
    18301 gatgcaaccc agagcgtggg caaactgcca atcgacctga gccagttgaa agttgacctg
    18361 atgtctttct ccggtcacaa aatctatggc ccgaaaggta tcggtgcgct gtatgtgcgt
    18421 cgtaaaccgc gtgtacgcat cgaagcgcaa atgcacggcg gcggtcacga gcgcggtatg
    18481 cgttccggca ctctgcctgt tcaccagatc gtcggaatgg gcgaagccta tcgcatcgca
    18541 aaagaagaga tggcgaccga gatggaacgt ctgcgcggcc tgcgtaaccg tctgtggaac
    18601 ggcatcaaag atatcgaaga agtttacctg aacggtgacc tggaacacgg tgcgccgaac
    18661 attctcaacg tcagcttcaa ctacgttgaa ggtgagtcgc tgattatggc gctgaaagac
    18721 ctcgcagttt cttcaggttc cgcctgtacg tcagcaagcc tcgaaccgtc ctacgtgctg
    18781 cgcgcgctgg ggctgaacga cgagctggca catagctcta tccgtttctc tttaggtcgt
    18841 tttactactg aagaagagat cgactacacc atcgagttag ttcgtaaatc catcggtcgt
    18901 ctgcgtgacc tttctccgct gtgggaaatg tacaagcagg gcgtggatct gaacagcatc
    18961 gaatgggctc atcattaatc ggtatcggaa tcaggagaat ttataatggc ttacagcgaa
    19021 aaagttatcg accattacga gaatccgcgt aacgtgggtt cctttgataa caacgacgag
    19081 aacgtcggca gcggcatggt gggtgcaccg gcctgtggcg acgtaatgaa gttgcagatt
    19141 aaagtcaacg atgaaggtat cattgaagac gcgcgtttta aaacttacgg ctgcggttcc
    19201 gctatcgctt ccagctccct ggtcaccgaa tgggtgaaag ggaagtctct cgacgaagcg
    19261 caggcgatca aaaacaccga tattgctgaa gaacttgaac tgccgccggt gaaaattcac
    19321 tgttctattc tggcagaaga cgcgatcaaa gccgccattg cggactataa aagcaaacgt
    19381 gaagcaaaat aagagttgag gtttggttat gtcgattaca ctgagcgaca gtgcagcagc
    19441 gcgagtaaat acctttctgg ctaaccgcgg caaagggttt ggcctgcgtc tgggcgtgag
    19501 aacctccgga tgttcaggta tggcttatgt actggaattt gttgacgaac cgacgccgga
    19561 agacatcgtg tttgaagaca aaggcgtgaa agtcgtggtc gatggcaaaa gcctgcaatt
    19621 tctggacggt acgcagctgg acttcgtaaa agaaggcctg aatgaagggt ttaaattcac
    19681 caacccgaac gttaaagatg agtgtggttg cggcgaaagc ttccacgttt gatgcgcata
    19741 ccgataaccc caccgtggtc gcctgcgcgt ggggtttgtt ttacctgatt cgccgttatc
    19801 gcggcggatc gcagccctga gaatgttatg gattacttca ccctctttgg cttgcctgcc
    19861 cgttatcaac tcgataccca ggcactgagc ctgcgttttc aggatctaca acgtcagtat
    19921 catcctgata aattcgccag cggaagcctg gcggaacaac tcgccgccgt acagcaatct
    19981 gcaaccatta accaggcctg gcaaacgctg cgtcatccgt taatgcgcgc ggaatatttg
    20041 ctttctttac acggttttga tctcgccagc gagcagcata ctgtgcgcga caccgcgttc
    20101 ctgatggaac agttggagct gcgcgaagag ctggacgaga tcgaacaggc gaaagatgaa
    20161 gcgcggctgg aaagctttat caaacgtgtg aaaaagatgt ttgatacccg ccatcagttg
    20221 atggttgaac agttagacaa cgaggcgtgg gacgcggcgg cggatacggt gcgtaagctg
    20281 cgttttctcg ataaactgcg gagcagtgcc gaacaactcg aagaaaaact gctcgatttt
    20341 taatttctgg aagctaaaca tggccttatt acaaattagt gaacctggtt tgagtgctgc
    20401 gccgcatcag cgtcgtctgg cggctggtat tgacctgggc acaaccaact cgctggtggc
    20461 gacagtgcgc agcggtcagg ccgaaacgtt agccgaccat gaaggccgtc acctgctgcc
    20521 atctgttgtt cactatcaac agcaagggca ttcggtgggt tatgacgcgc gtactaacgc
    20581 agcgcttgac accgccaaca ccattagttc tgttaaacgc ctgatggggc gctcgctggc
    20641 tgatatccag caacgctatc cgcatctgcc ttatcaattc caggccagcg aaaacggcct
    20701 gccgatgatt gaaacggcgg cggggctact gaacccggtg cgcgtttctg cggacatcct
    20761 caaagcactg gcggcgcggg caactgaagc cctggcaggc gagctggatg gtgtagttat
    20821 caccgttccg gcgtactttg acgatgccca gcgtcagggc accaaagacg cggcgcgtct
    20881 ggcggggttg catgttctgc gcttacttaa cgaaccgacc gctgcggcta tcgcctacgg
    20941 gctggattcc ggtcaggaag gcgtgatcgc tgtttatgac ctcggtggcg ggacgtttga
    21001 tatttccatt ctgcgcttaa gtcgcggcgt gtttgaagtg ctggcaaccg gcggtgattc
    21061 cgcgctcggc ggcgatgatt tcgaccatct gctggcggat tacattcgcg agcaggcgga
    21121 cattcctgat cgtagcgata accgcgttca gcgtgaactg ctggatgccg ccattgcagc
    21181 caaaatcgcg ctgagcgatg cggactccgt gaccgttaac gttgcgggct ggcagggcga
    21241 aatcagccgt gaacaattca atgaactgat cgcgccactg gtaaaacgaa ccttactggc
    21301 ttgtcgtcgc gcgctgaaag acgcgggtgt agaagctgac gaagtgctgg aagtggtgat
    21361 ggtgggcggt tctactcgcg tgccgctggt gcgtgaacgg gtaggcgaat ttttcggtcg
    21421 tccaccgctg acttccatcg acccggataa agtcgtcgct attggcgcgg cgattcaggc
    21481 ggatattctg gtgggtaaca agccagacag cgaaatgctg ctgcttgatg tgatcccact
    21541 gtcgctgggc ctcgaaacga tgggcggcct ggtggagaaa gtgattccgc gtaataccac
    21601 tattccggtg gcccgggctc aggatttcac cacctttaaa gatggtcaga cggcgatgtc
    21661 tatccatgta atgcagggtg agcgcgaact ggtgcaggac tgccgctcac tggcgcgttt
    21721 tgcgctgcgt ggtattccgg cgctaccggc tggcggtgcg catattcgcg tgacgttcca
    21781 ggtcgatgcc gacggtcttt tgagcgtgac ggcgatggat aaatccaccg gcgttgaggc
    21841 gtctattcag gtcaaaccgt cttacggtct gaccgatagc gaaatcgctt cgatgatcaa
    21901 agactcaatg agctatgccg agcaggacgt aaaagcccgt atgctggcag aacaaaaagt
    21961 agaagcggcg cgtgtgctgg aaagtctgca cggcgcgctg gctgctgatg ccgcgctgtt
    22021 aagcgccgca gagcgtcagg tcattgacaa tgctgccgct cacctgagtg aagtggcgca
    22081 gggcgatgat gttgacgcca tcgaacaagc gattaaaaac gtagacaaac aaacccagga
    22141 tttcgccgct cgccgcatgg accagtcggt tcgtcgtgcg ctgaaaggcc attccgtgga
    22201 cgaggtttaa tatgccaaag attgttattt tgcctcatca ggacctctgc cctgatggcg
    22261 ctgttctgga agctaatagc ggtgaaacca ttctcgacgc agcgctgcgt aacggtatcg
    22321 agattgaaca cgcctgtgaa aaatcctgtg cttgcaccac ctgccactgc atcgttcgtg
    22381 aaggttttga ctcactgccg gaaagctcag agcaggaaga cgacatgctg gacaaagcct
    22441 ggggactgga gccggaaagc cgtttaagct gccaggcgcg cgtcaccgac gaagatttag
    22501 tggtcgaaat cccgcgttac actatcaacc atgcgcgtga gcattaacag aggttagtat
    22561 gggacttaag tggaccgata gccgcgaaat tggcgaagca ctgtacgatg cgtatcccga
    22621 tcttgatccg aaaacggttc gattcaccga tatgcatcag tggatttgcg atctggaaga
    22681 tttcgacgac gacccgcagg catccaacga gaaaatcctc gaagcgattt tgttagtctg
    22741 gctggacgaa gccgagtgaa tgaacgggct gcctgggggt agcccgtttt gctgttaaac
    22801 gaataaggat aactaaaatg acagaagcga tgaagattac cctctctacc caacctgccg
    22861 acgcgcgctg gggagaaaaa gcaacttaca gcattaataa tgacggcatt accctgcatt
    22921 tgaacggggc agacgatctg gggctgatcc agcgtgcggc ccgcaagatt gacggtctgg
    22981 gcatcaagca tgttcagtta agcggtgaag gctgggatgc ggatcgctgc tgggcattct
    23041 ggcaaggtta taaaggcccg aaaggcacgc gtaaagtgga gtggccggat ctggacgatg
    23101 cccagcgcca ggaactggat aaccgcctga tgatcatcga ctgggtgcgt gacaccatca
    23161 acgcaccggc agaagaattg ggaccatcgc aactggcaca gcgtgctgtt gatctgatca
    23221 gcaacgtcgc gggcgatcgt gtgacttatc ggatcaccaa aggcgaagat ctgcgtgagc
    23281 aaggttatat ggggctgcac actgtcgggc gcggttcaga acgttctccg gtattgctgg
    23341 cgctggatta caacccaact ggcgataaag aagcaccggt gtacgcgtgc ctggtaggta
    23401 aaggtatcac ttttgactcc ggcggctaca gcatcaaaca gactgcgttt atggactcga
    23461 tgaagtcgga catgggcggc gcggcaacgg ttaccggggc gctggcattt gccattacgc
    23521 gcggactgaa caagcgcgtg aagctgttcc tctgctgtgc ggataacctg attagcggca
    23581 atgcgttcaa gctgggcgat atcatcacct atcgcaacgg taaaaaagtt gaagtgatga
    23641 acactgatgc cgaagggcgt ctggtgcttg ccgatggtct gattgatgcc agtgcgcaga
    23701 aaccggaaat gatcattgat gcggcgaccc tcaccggggc ggcgaaaact gcgctgggta
    23761 atgattatca cgcgctgttc agttttgacg atgcgcttgc cggtcgttta ctggcgagtg
    23821 cctcacaaga gaacgaacca ttctggcgtc tgccgctggc ggaattccac cgcagccagc
    23881 tgccgtctaa ctttgccgaa ctgaacaata ccggaagcgc ggcgtatccg gcaggcgcga
    23941 gcacggcggc gggcttcctg tcgcactttg ttgagaacta tcagcaaggc tggctgcata
    24001 tcgactgctc ggcgacttac cgtaaagcgc cggttgaaca gtggtctgcg ggcgctacgg
    24061 gacttggtgt gcgcacgata gctaatctgt taacggcgta aactaatatg ccggatgcgg
    24121 cgtaaacgcc ttatccggcc tacaaattct gcaaattcaa aaaattgcga atcccttgta
    24181 ggcctgataa gcgtagcgca tcaggcattt gcgccccgaa cgttgaatga attgtggttc
    24241 ttatgtccga aacaaaaaac gaacttgaag acctgctgga aaaagcagca accgaaccgg
    24301 cgcaccgccc ggcctttttc cgtactctac tggaatccac cgtctgggtg cctggtacgg
    24361 cggcgcaggg cgaggctgtg gttgaagata gtgcgcttga tttacagcac tgggaaaaag
    24421 aagacggcac cagcgtcatt ccttttttca cctcgttaga agcgcttcaa caggcggttg
    24481 aagacgaaca ggcatttgtc gtaatgcccg ttcgcacatt gtttgagatg acccttggcg
    24541 aaacgctctt ccttaatgcc aaactgccga ccggtaaaga atttatgccg cgtgaaatca
    24601 gtttgttgat tggtgaagag ggaaatccgc tgagcagcca ggaaatcctg gaaggcggtg
    24661 aatcgctgat attatcggaa gtcgcagagc cgccagcaca aatgattgat tcactcacca
    24721 ccttatttaa aaccattaag ccggtgaagc gtgcttttat ttgttcaatt aaagagaacg
    24781 aagaggcaca gcctaattta cttattggca ttgaagccga tggtgatatc gaagaaatta
    24841 ttcaggcgac gggaagtgta gcgaccgata cattacctgg cgatgaacca atcgatattt
    24901 gtcaggtgaa aaaaggggaa aaaggaatta gccactttat taccgaacat attgcgccat
    24961 tctatgaacg tcgctggggt ggttttttgc gtgactttaa acaaaaccgg ataatctaaa
    25021 atatgccgcc ctaaagggcg gcattcgggc ttacttgctg ctctgaaggt tgttaaccac
    25081 cagtaaattg tcgccggaga gactccggtt attatttact tgggaaaaca gcgcaccaga
    25141 aacaataagt acgatacaga agcaccgtat cttcattgtg ctgtcgtgat gcgaagaccg
    25201 catcacgaca gcaccactgg cgatattgcc gcgatagcaa gcgctgagac gcgcttgctc
    25261 taacggtcgc ggaacatcct ttccttaagc cagcgcccgc ttgcgagacg gcggaaaggg
    25321 ccgcagtgga taatacacta acagaacaat aataactctc ttttgcttga caaaaagaga
    25381 gttactggcg agtattgtct ccgctgagac gcgcttgcat tactacggta atacaacctg
    25441 ctggaaaggc cgcgaaccgg accagcaaca aataaccgcc tgattttggc ggttttttgt
    25501 tttttaaagg aaagtaaatt tacatgttta agtaatatct tttaccggtt agaattttta
    25561 attttgagca ggatttgttt atatttgtct gaattcggac taataagcca ggttatttta
    25621 ttaccgaaca cggcggaaag ggccggtgtc gataatacgc taaataaaca ataattactc
    25681 tcttttgctt gacaaaaaga gagttactgg tgagtattgt tttactgtga agcacctgcg
    25741 ttgctcatgt aacacaagtc aacctgctgg aaaggccgcg aaccagacca gcaataaaaa
    25801 aacgccaaat tcggcggttt tttattgcct gtaaaatact tatttcactg gctcaaccgg
    25861 taaatctgcc cgcgcgcccc attcactcca tgcgccgtcg tacagtttca cgtttggcac
    25921 atccagcgtc gcgagtgcta acaaaaccac ggctgccgtt acaccagagc cgcagctgac
    25981 gataatcggt ttgtcatagc tgacgccgcg accaaaaaat atcgcatcca gttcatcggt
    26041 cgtttttagt tcgccttcgc gcaccagttc cgtccacgga acgttcaatg caccgggaat
    26101 atgtccgcga cgtaaacctg ggcgaggttc atcaacttct gcgttaaaac gtgcagccgg
    26161 gcgggcatca ataatttgcg ccgtattttc atggcttgcc aataacacat cggttacttt
    26221 caccactgct tcaggattaa acgcggcgtt aaactctcct tccggcagct ctactgcacc
    26281 ttcttctaat agcaggtcat cgcgctgcca gcctgcaagt ccccgttact ttattcgtac
    26341 cccttataat ggggtgttag ccagccagac ccggcatgat tactgccccc agtcgtccat
    26401 gatccggggg gg
//
LOCUS       DAJTYG010000052        25410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTYG010000052 DAJTYG010000000
VERSION     DAJTYG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.052"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(1..2441)
                     /locus_tag="OO568_002314"
                     /pseudo
     CDS             complement(1..2441)
                     /locus_tag="OO568_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
     gene            complement(2533..3033)
                     /locus_tag="OO568_002315"
     CDS             complement(2533..3033)
                     /locus_tag="OO568_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6641649.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            complement(3063..3779)
                     /locus_tag="OO568_002316"
     CDS             complement(3063..3779)
                     /locus_tag="OO568_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6641650.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            4075..4851
                     /gene="exuR"
                     /locus_tag="OO568_002317"
     CDS             4075..4851
                     /gene="exuR"
                     /locus_tag="OO568_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR6641651.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            5196..5858
                     /locus_tag="OO568_002318"
     CDS             5196..5858
                     /locus_tag="OO568_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6641652.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            5862..6245
                     /gene="mzrA"
                     /locus_tag="OO568_002319"
     CDS             5862..6245
                     /gene="mzrA"
                     /locus_tag="OO568_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6641653.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            6392..6746
                     /locus_tag="OO568_002320"
                     /pseudo
     CDS             6392..6746
                     /locus_tag="OO568_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            6784..7089
                     /locus_tag="OO568_002321"
     CDS             6784..7089
                     /locus_tag="OO568_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6641654.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            7092..7496
                     /locus_tag="OO568_002322"
     CDS             7092..7496
                     /locus_tag="OO568_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6641655.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            7486..7785
                     /locus_tag="OO568_002323"
     CDS             7486..7785
                     /locus_tag="OO568_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6641656.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            7971..8363
                     /locus_tag="OO568_002324"
     CDS             7971..8363
                     /locus_tag="OO568_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6641657.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFSFLTRTTTLFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            8433..9419
                     /gene="yqjG"
                     /locus_tag="OO568_002325"
     CDS             8433..9419
                     /gene="yqjG"
                     /locus_tag="OO568_002325"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6641658.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            9712..10080
                     /locus_tag="OO568_002326"
     CDS             9712..10080
                     /locus_tag="OO568_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6641659.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(10287..11183)
                     /gene="yhaJ"
                     /locus_tag="OO568_002327"
     CDS             complement(10287..11183)
                     /gene="yhaJ"
                     /locus_tag="OO568_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR6641660.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYIMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFSLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGQLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            11288..11989
                     /locus_tag="OO568_002328"
     CDS             11288..11989
                     /locus_tag="OO568_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6641661.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            12012..12176
                     /locus_tag="OO568_002329"
     CDS             12012..12176
                     /locus_tag="OO568_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641662.1"
                     /translation="MSKKSTKKRQLAKPVVAKEPARTAKNFGYEEMLSELEAIVTDAE
                     TRLAEDEATA"
     gene            complement(12388..13680)
                     /locus_tag="OO568_002330"
     CDS             complement(12388..13680)
                     /locus_tag="OO568_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6641663.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGITVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGAKVSTSASAAWKAVLMALDDTAVTGNE
                     GIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(13708..14985)
                     /locus_tag="OO568_002331"
     CDS             complement(13708..14985)
                     /locus_tag="OO568_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641664.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFGVYLGFREATQGIVMNILR
                     RKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTSICSPIFGMVGCLIPAWLV
                     YKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(15260..16624)
                     /gene="tdcG"
                     /locus_tag="OO568_002332"
     CDS             complement(15260..16624)
                     /gene="tdcG"
                     /locus_tag="OO568_002332"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6641665.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCD"
     gene            complement(16696..17085)
                     /locus_tag="OO568_002333"
     CDS             complement(16696..17085)
                     /locus_tag="OO568_002333"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6641666.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGRMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(17099..19392)
                     /gene="tdcE"
                     /locus_tag="OO568_002334"
                     /pseudo
     CDS             complement(17099..19392)
                     /gene="tdcE"
                     /locus_tag="OO568_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
     gene            complement(19426..20634)
                     /gene="tdcD"
                     /locus_tag="OO568_002335"
     CDS             complement(19426..20634)
                     /gene="tdcD"
                     /locus_tag="OO568_002335"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6641667.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWIASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(20660..21991)
                     /gene="tdcC"
                     /locus_tag="OO568_002336"
     CDS             complement(20660..21991)
                     /gene="tdcC"
                     /locus_tag="OO568_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6641668.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKVQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(22013..23002)
                     /gene="tdcB"
                     /locus_tag="OO568_002337"
     CDS             complement(22013..23002)
                     /gene="tdcB"
                     /locus_tag="OO568_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6641669.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(23101..24039)
                     /gene="tdcA"
                     /locus_tag="OO568_002338"
     CDS             complement(23101..24039)
                     /gene="tdcA"
                     /locus_tag="OO568_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6641670.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            24228..24572
                     /gene="tdcR"
                     /locus_tag="OO568_002339"
     CDS             24228..24572
                     /gene="tdcR"
                     /locus_tag="OO568_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6641671.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            24828..25367
                     /locus_tag="OO568_002340"
     CDS             24828..25367
                     /locus_tag="OO568_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641672.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
BASE COUNT         6735 a         6419 c         5830 g         6426 t
ORIGIN      
        1 aacctgaatc gactccggta attgtgaata agtcaccaca ctacaagtaa catctccgct
       61 gtaacgaatg ctggcgcgtt tctttacaac actcagtgga cacttaagga attgctgccc
      121 ggaaagcaga tagagctctt tttcgctgcc gttaatttca agtgtaaatc cgccatcttc
      181 agctaccagt aacatggcgc tgttcagccc cacaatcggg aattttttca ccgctgccgg
      241 cagtaataaa cgaccgatat agttgtagtt aaagctggca gacacttcgc gatagcgcag
      301 cttgccaggc aacaacatta attcacgatt ccctgcaccg ttaatgatgt ttgttgtccc
      361 ctgctgattg ccctggctac tgtcgttaac ctcaacaaaa ccagactgaa atccaggata
      421 agggaacaac acactgtcac tttgaccaag ctgcaaagtt cggctgcctg ccacccgcgc
      481 ttcgagttgc agatcctgac tctcttcatc ctgttcagag aagcctttca catccaccag
      541 taccgcaccc agcaaatcgt ctcggtgttc aggcctccga cggttacgcc aagttcttta
      601 tggctgaatg aatcgttata aaaggtatgc gataactgcc atgacgtttg atcgccatcc
      661 tgttcgctat aacgataatc cgtagacaca ttggttgaat ggctgttgtt attgctatcc
      721 atcgttggcg tgtcagataa cgaaaacgat aagtacaagc cgttgtcgtc gttatcgcca
      781 ccgttgtcat tacgatggaa aacaccgact gacgtactca aaatccagtc acgcatcaca
      841 atagcgcggg aagcgctaag ttgtacagtt tcagagatac tttgcgctgt tgtctggcga
      901 agatagttat tttcaaagct ctgctgttta tcccaacgca aactatcatc agtgttattt
      961 tgtgtgcagg tataacccag catcatattc cagccgacaa aattcagtga aactgtcgcg
     1021 gaaatacttt cataacagct taaggcgtta tagctctctc tgctgtcggt tgtacaagca
     1081 tcgccgtcag agttggtccg ataaaagccc aacgtcggcc atcccggatt actccagttg
     1141 gcctgttgca tatcaccttt gccgccgtca tcgttacgga acacgctggc gctgattgca
     1201 agattcccca ccccgcctaa atctgccgtc cagttattac ctaactcgaa agcactcaca
     1261 tcatcggcat tcgccagccc tgcgtagagc tcatattgcg gatgtaatgg caggcgtact
     1321 cctagctgat aagctgaact ttcatcatca gaaacctgtg atgtagtttt acctgcctgt
     1381 aagaaccatt gcgcatttcc gtcagtcaga ccgccggttt tggtaaacgg cacaagctcg
     1441 gtgcgggtga gttggttatt ttcgtagact tttaatgcca cgctgtagct gcctggcgga
     1501 aaggaactgg tatcaataaa ttgcgaacca ctattgagat aaaacgatcc caaaagttgc
     1561 tcattacgat aagcgtcaac acgagaattg cgcgaaagca gaaccattac tggggttcct
     1621 tgctggcttt gcgcctggtt taaatagctc agtgtcgacc cgatacgcat cccatcgatt
     1681 gcaccgagtg gcagaaagtt aaaggtaaag ttcccgcctt gtgcattaaa tagtgtgcgg
     1741 ttgtccatgc gacccgcctg cacataataa cgacgtaaaa aatcataacg ataatagaga
     1801 tcgctgacgt cagtattact gtcactgaca tcatctgcat cataaccgtt gaaattccag
     1861 tgtgcaccaa tatagctatt ttctgttatt cccagcgcac cgtttccctg aatagacaac
     1921 gattggtaat catcctgtgc cagaacattg atgtcttgct gatgtataaa agcgttaaca
     1981 gtatttttgt ctggatttaa atataatgac ttcgcatcga aagctgaatt ccattgcgga
     2041 ttaataaaca acgttgccga actttcttca tcattataga ttatttccag cgtatcggta
     2101 ttcagaaaac cacaccctga gtcagtacgt acataaccgc aagccaattc accgtggcga
     2161 gctagcggct gcgataattt ttctttcact atatgcgcga ttttctgatc gtctacatcc
     2221 agttccagct tgttatatag acttgctgga tcaagaaatg ttacggtatc aagattaacc
     2281 attgcataat atttaccgag acttttccct gaaaaataaa cctcgatata ctcctgctgt
     2341 ccctgagcaa taacctcaaa ccctgcggga attttggtta atgtcgcctc tgccgggctg
     2401 agactcgcca ggatcaaaag agccagtaat tttttatcca tggcaatcct gaaatccata
     2461 acaaatacta aaacagttaa aataagggag ggctacccct ccctttgtta cgtgttaatt
     2521 ctgttatccg gattaaatat cctgagacag ataaatgctt actacaccac ggtagatgcc
     2581 tgtttccaga acacctttgg tggtttgagc aaacatcagg tttttagcca gagaaccgtt
     2641 agttaacgca tcggaagcaa agattttggt tgcggtaaaa gtggttgccg catcagcttt
     2701 aatttcttca ccaccccagg ttactgtcag cggaacaatc ttgctagcat ccaggctttg
     2761 aaccagttgc gctggggaca ctaactgcat ttttacatct ttggtaacgt cgttagacca
     2821 gattttggtc atcaactggt aagattgcag accctgcccc ggcagatatt gcatttctac
     2881 tgctttcggc aacgcggtgt tatcggtctg tgtcatatcc agagcggcat caacgttggc
     2941 cgttacagta atatcctttt gtactgccaa tgcggagcct gcaacagaaa acatcgctgc
     3001 gaccagaaga gattttgcaa ataccttttt cattactatt ccttcgttta ttacaatgat
     3061 aattaatgga ccgccagttc aaactcgcca ttatctttgg tgatccagtt attatatttg
     3121 acaacgattt tgtcgtagcc agatatgctt tcaagatttc tttcctgacc agggaagatg
     3181 tttttattta cggtctgtat cttacagttt tctttgctat tatttttaca ataagcgatc
     3241 gttaaaagct ttaagcgttg attccctgca ttaattaatt tttgggcaga atttacttct
     3301 aacttagcta taggttgctg aggtggaaca ctaacaacga ttccccaaat taagttaact
     3361 gttaactgca tagagagatc ttttttatct gtggcaacgt catcaatact gggaaccgct
     3421 tcaaatttaa cgcgataatt tttctctttt tgctctggtt ccatcgcaac aaaacgtacg
     3481 gttttgctac taccggctgg taaagcgaat ttaggtggca taacaaccag atgatttgca
     3541 tcaccggatt taatctcaac ttcattttcc tgaggcgtcg atggattatc gatacggaat
     3601 actgtcgcct tgatgtactg aacttcatta cttttagata ttacgcgaac gttgccttca
     3661 ccctgactat taatggaaac aggcatagga taaacagtca tatttgactg tgccaaaaaa
     3721 ggagcgaaca cgaataaaag cggcgtaagc cgtaataaga gattaatttt cgccaccata
     3781 taattttgat cttacaatat cgtttcaaaa aagttggtgt attagagatg tagatttatt
     3841 gtaagtcaat ataaaaaagc atttttagtt aacattaaat ttaaaggctg ggaattaact
     3901 tactgaaatt tagtagcttt cacaatgacg atacttattt tttaggaatt tataaatatt
     3961 cacaatatta atttaataca ttaattcaat catcaaattt atatatgacc aatggcatac
     4021 ccatgaataa agtggtatga taacttcgtc cgcattccac tccgggagac gcatatggaa
     4081 atcactgaac cacgccgttt gtatcaacaa cttgctgctg acctgaaaga gcgcattgaa
     4141 cagggcgtct atctggtggg tgataaactg cctgccgaac gctttattgc cgatgaaaag
     4201 aacgtcagcc gaacggtagt tcgtgaagcc atcatcatgc tggaagttga aggctatgtg
     4261 gaggtgcgta aaggttcagg tattcatgtg gtttccaacc agccgcgtca tcagcaggcg
     4321 gctgacaata atatggaatt cgccaattac ggtccgtttg agttgcttca ggctcgccag
     4381 ctcatcgaaa gtaatattgc cgagttcgcg gcaactcagg taacgaaaca agacatcatg
     4441 aaactgatgg ccattcagga acaggcgcgc ggcgaacaat gcttccgtga ttccgagtgg
     4501 gatttacagt tccacattca ggtagccctg gcgacgcaga actccgccct ggcggccatc
     4561 gttgaaaaaa tgtggaccca gcgtagccat aacccgtact ggaaaaaact acacgaacac
     4621 attgattccc gtaccgttga taactggtgt gatgaccacg atcaaattct caaggcgtta
     4681 attcgcaaag atcctcatgc tgctaagctg gcaatgtggc agcatctgga aaacaccaaa
     4741 atcatgttat ttaacgaaac cagcgacgat ttcgagttca atgccgaccg ctatctgttc
     4801 gccgaaaacc cggtagtgca tctcgatacg gccaccagcg gcagcaaatg aaattcctgt
     4861 ccgacaggcg ctgcaatgcg cctgtttgtc tcgtcggtaa gcgaaaagta taaagtgtca
     4921 gcctgtgtaa atcctctcgc caccctcccc tgcaatcagc aaaatcagac tccacggaca
     4981 tgtaattttg ataacgaaca acgttgacct ttgttacaat tagattcaat ttgaatttat
     5041 gcttttgaat gctttcttat ctcacgtttt aacagggaat agttcaggct gtgttgatgt
     5101 atcaaacccg cagaacatac caaaacagca ataacattgc ggtagtgcat cttttaaaac
     5161 cagcgtggcg ttaaccgatt caccaggaat aatgaatgga acttttgacc caattgctgc
     5221 aagccctgtg ggcgcaggat tttgaaaccc tggccaatcc atcgatgatt ggcatgttgt
     5281 attttgtctt gtttgtaatt ttgttccttg aaaatggctt gcttccggcg gcctttttac
     5341 cgggcgacag tttactggta ttggtcggcg tgttgattgc gaaaggcgcg atgggttacc
     5401 cgcaaacgat tctgctgctg accgttgccg ccagcctcgg ttgctgggtc agctatattc
     5461 aggggcgatg gctgggcaat acccgcaccg tacaaaactg gctatctcat ttacccgcgc
     5521 attatcatca acgcgcacac catctttttc ataaacacgg tttatcggcg ctgttaattg
     5581 gtcgctttat tgcatttgtc agaacactgc tgccgacgat tgccgggtta tcagggctga
     5641 ataacgcgcg ctttcagttc ttcaactgga tgagcggtct gctgtgggta ttgatcctga
     5701 caactctggg ttacatgctc ggcaaaacgc cggtattttt aaagtacgag gaccagctga
     5761 tgtcatgcct gatgctgctc ccggtggtgc tgctggtgtt tggcctggca ggttctctgg
     5821 tcgtgttatg gaaaaagaaa tatggaaatc gggggtaagg gatgcaaata cctcgcatgt
     5881 cgcttcgcca gctagcctgg tccggcactg ttttacttct ggtcggcacg ctgttgctgg
     5941 cctggtccgc ggttcgccag caagagtcta cgctggcgat tcgtgccgtt catcaaggca
     6001 caacgatgcc agacggtttt tcgatctggc atcaccttga cgctcatggc attcctttca
     6061 aaagtatcac ccccaaaaac gacactctgt taattacatt tgactccagc gaccagagcg
     6121 ccgccgcaaa agcggtcctc gacagaacat tgccccatgg ctacatcatt gcgcagcagg
     6181 acaataacag tcaggctatg cagtggctga cccggttacg ggataactct catcgcttcg
     6241 gataacttcc tgttatccga aacatatcac tcactttggt gatttcaccg taactgtcta
     6301 tgattaatga ggcggtggtt aacactaccg cttcgttgtc tgaacaatcg ttcctttgta
     6361 ataggtccga atcacaatgg aaggttcaag aatgaaatac cgcatcgctt tagctgtttc
     6421 tctctttgct cttagtgccg gtagttatgc cactaccctg tgtcaggaaa aggagcaaaa
     6481 tatccttaag gagatcagct atgccgaaaa acaccaaaac cagaatcgta ttgacggtct
     6541 gaataaagcc ctgagtgaag tccgggccaa ctgttcagat agccagctgc gtgccgatca
     6601 tcacaagaaa atcgcaaagc agaaagatga ggtggcggaa cgccagcaag atttagccga
     6661 ggcgaagcaa aaaggcgatg ccgatagaac ggaaactggc agaagcgcag gaagagctga
     6721 aaaagctgga agcgcgcgac tactaactca caatagtcac tacttactca cctggagaaa
     6781 actatgtcga aagaacacac tacggaacat ctgcgtgctg agttgaaatc cctttccgat
     6841 acgctggaag aggtgcttag ctcatctggc gagaagtcga aagaagagtt gagtaagatt
     6901 cgtagcaaag cggagcaggc actgaaacag agccgttatc gcctgggtga aaccggtgat
     6961 gccatcgcca aacaaacccg tgtcgcggcg gcgcgtgctg atgagtatgt gcgtgaaaat
     7021 ccgtggaccg gcgtgggaat tggcgctgca atcggtgtag tgctcggcgt tctgctgtcg
     7081 cgtcgttaat tatggcggac actcatcacg cgcaagggcc cggtaaaagc gttctgggca
     7141 tcgggcagcg aattgtttcc atcatggttg aaatggtgga aacacgtctg cggctggcgg
     7201 tggtggagct ggaagaggaa aaagctaatc tctttcaact tttactgatg ctgggcctga
     7261 cgatgctttt cgctgcattt ggtcttatga gcctgatggt gctaattatt tgggcggttg
     7321 atccgcaata tcgcctgaat gcgatgattg ccaccaccgt ggtgttgctg ctactggcac
     7381 tgattggcgg tatctggacg ttacgtaaat cgcgtaaatc caccttgctg cgccatacac
     7441 gccatgagct ggcaaacgat cggcaactgc tcgaggagga gtcccgtgag cagtaaagtc
     7501 gaacgtgaac gacgtaaggc gcaactgctt agccagatcc agcaacaacg gctggatctt
     7561 tccgccagtc gtcgtgaatg gctggaggca acaggcgctt acgatcgtcg ctggaatatg
     7621 ctgctaagtc tgcgctcctg ggcgctggtt ggcagtagcg tgatggcgat ctggacgatt
     7681 cgccatccta atatgctggt ccgctgggcc agacgcggtt ttggcgtatg gagcgcctgg
     7741 cgtctggtta aaacgaccct caagcagcaa cagcttcgcg gttaacttcc ctctggccgg
     7801 agcaattccg gccttatccc tcaaattttt tgaatatttt tgacagtttt ccttgctaac
     7861 aatcatcatt caccacgttt atgattctct ccatcgacag caacgacgct aataccgcgc
     7921 ccttgcacaa aaaaacaatc agcagcctga gtggcgcagt ggagagtatg atgaaaaaat
     7981 tagaagatgt tggtgtactg gtagcgcaca ttttaatgcc gattctgttt attaccgcag
     8041 gctggggaaa aatcaccgct tactctggta cccaacaata tatggaagca atgggtgtcc
     8101 cgggttttat gctgccgctg gtgattctgc ttgagttcgg tggtggtctg gcaatcctat
     8161 tcagtttcct gactcgcacc acaaccctgt ttactgcggg ctttacgctg ctgacggcat
     8221 ttttatttca cagcaacttt gctgaaggcg tcaactcgct gatgttcatg aaaaacctga
     8281 caatttctgg cggattcctg ctgctggcaa ttaccggtcc gggcgcgtat agcatcgacc
     8341 gcctgctgaa taaaaagtgg taagcacgcg ctatacttaa cgataaaaaa cgaggaggaa
     8401 gctcctcgtt tttgctattg gaggagagaa aaatgggtca actgattgac ggcgtctggc
     8461 atgacacctg gtacgatacc aaatctaccg gcggtaaatt tcaacgttca gcttccgcat
     8521 ttcgtaactg gctcactgcc gatggcgcac ctggccccac tggcacaggc ggttttatcg
     8581 cagagaaaga tcgttatcat ctctatgttt cactcgcctg cccgtgggcg caccgcacgc
     8641 tgatcatgcg caaactcaaa ggactggaac cgtttatttc cgtttccgta gtgaacccgc
     8701 tgatgctgga aaacggctgg acctttgatg acagttttcc gggagcaacc ggagatacgc
     8761 tctatcaaca tgaatttctg tatcagcttt atctccacgc cgatccacac tacagcggac
     8821 gagttactgt tcccgtgctg tgggacaaaa agaaccacac catcgtcagc aacgaatcag
     8881 cagaaatcat ccgcatgttc aataccgcgt ttgatgcgct gggcgcaaaa gcgggtgatt
     8941 actacccacc agccctgcaa acgaaaattg acgaacttaa cggctggatt tatgacaccg
     9001 ttaacaacgg cgtgtataaa gctggttttg ccaccagcca gcaagcctac gacgaggcgg
     9061 tggcgaaagt gtttgaatcg ctggcgcgac tggagcagat tttaggccag catcgttacc
     9121 tgaccggcaa ccagctaacc gaagccgata ttcgcctgtg gactacgctg gtgcgttttg
     9181 atccagtgta tgtgacccac ttcaagtgtg ataagcaccg catcagcgat tacctgaatc
     9241 tgtatggctt cctgcgcgat atctaccaga tgccggggat cgccgaaact gtcaatttcg
     9301 atcacatccg taatcattac ttccgcagcc ataagaccat caaccctacg gggattattt
     9361 caattggtcc gtggcaggat ctcgatgaac cgcatggacg agatgttcgc ttcggttaaa
     9421 aaaaggcacc ttacgaggtg cctctgataa ttagctaagt gcatgttgtt catgtcggat
     9481 gcggcgtgaa cgccttatca aacctacaaa gcgtttatat tcaatatgtt acaatgaccg
     9541 cgtaggccag ataagcgtag cgcatctggc gattttgcgc ttgccatcag tcttagcgcg
     9601 ccttcacggc actttattaa ttccttgaac gaatatttac tgccatttca ttcacgtcta
     9661 ttcttaattt gctgctttaa aaacaagtga ttgagcaaaa ttgaggcaaa aatggactgg
     9721 tatctgaaag tactaaaaaa ttatgtcggt ttccggggtc gtgcgcggcg caaagagtac
     9781 tggatgttta ttctggtcaa catcatcttt acgttcgtgc tggggctgct ggataaaatg
     9841 ttaggctggc aacgcgctgg tggcgaaggc atcctgacga caatctacgg tattctggtg
     9901 tttttaccgt ggtgggcggt tcagttccgc cgcctgcacg acaccgaccg ctcggcgtgg
     9961 tgggcactgc tattcttaat cccgtttatc ggctggctta ttatcatcgt ctttaactgc
    10021 caggcgggta cgccaggcga aaaccgcttt ggaccagatc caaaactgga acaagaataa
    10081 tgattaagca ccttgtaaaa ggtgcttcaa attgttgaca aagtgcgctt tgtttatgcc
    10141 ggatgcggcg taaacgcctt atccagccta caaattgcca aaattcaata tattgcagtg
    10201 actgcgtagg cctgataagc gtagcgcatc aggcagtttt gcgtttgtca gcagtcagaa
    10261 gcaccttgtg aaaggtgctt aatagattat tttccagcga acaactttgg aatttcccgc
    10321 agacaccagg atttcgcttc ccccatactg tcacgacgcc aggccataat aatatcgatc
    10381 tcgctggtcg attccgggct gacgacacgc aactgccctt ccgcaatatc tttttcgacc
    10441 atcggatacg gcatcgtcgc cacgccaagc cccgccagta atgcctgacg tttatcttca
    10501 atcgtgctca ccgttaagcg cggctgtttg tccagcagct gtacggtcaa caccgggcgc
    10561 tcacgagcgg tatccgctac cgcaattcca cgatatttca cacgcgtcac ttcagataac
    10621 ggttccggct cctgatgaat cgggtgatca ggcgcggcaa cgtagacgtt cattaacgta
    10681 tagagcttgc gcgagttgat ctccgacgag gaacgaaaat gcatatccgg cgcgataacg
    10741 atatccgccc gcccctgctc cagccgttcc cacgcccccg ccagcacttc tgtgatgatt
    10801 gccagttggg tattggcttt tgccgccagt ttgtcgatta acgagaaaaa ggcaggtgtc
    10861 ggtaccagcg cttcggtcac aatggtgaga tgcgtttccc aaccgcgcgc gagagcttcc
    10921 gcatcagtag tcagtttatc tgcggcttcc agcaaaacgc gccctcgctc cagcaacatc
    10981 ctcccgacat tggtgaattt ggtgcgatgg cccgagcggt caaacagcac cacatccagc
    11041 tcttcttcca gtttttgcat gatgtagcta agtgcggaag gcacgcgtcc cagctcatcc
    11101 gctgccgccg caaaactgcc ccggcgatcg atcgcatcca taacccgtag tgcttccagc
    11161 gttaatgccc tttctttggc catttcgttc tcattcagga aatttgaaca taccgggcag
    11221 aatatctggc taacaatgta gcgtccagcc ccttaccata aaaggaagta aagagaggtc
    11281 aagaattatg attactaccc gaactgccag gcagtgtgga caagcagact acggatggtt
    11341 gcaggcccgg tatacttttt cctttggaca ctacttcgac ccgaaattgt taggctatgc
    11401 ctccctgcgt gtgcttaacc aggaagtgct ggccccaggt gccgcctttc agccgcgaac
    11461 ctatcccaaa gtcgatattt taaatgtgat tctggatgga gaagcagagt atcgcgacag
    11521 cgaaggcaat catgttcagg ccagcgccgg agaggcgttg ctgctctcta cccagccggg
    11581 tgtcagctat agcgaacaca atctcagcaa agacaaaccg ttaacgcgaa tgcagctttg
    11641 gctggatacc tgcccgcagc gagagaatcc gctgattcaa aagctggcgc ttaatatgga
    11701 caagcagcaa ttaatcgcct cgccagaggg tgcgatggga agcctgcaat tacgccaaca
    11761 agtgtggctg caccatatcg tgctcgacaa aggcgaaagt gcgaatttcc agttgcatgg
    11821 gccacgcgcg tatttgcaat cgattcacgg gaaatttcat gcgcttacgc atcatgaaga
    11881 gaaagcggcg ctaacctgcg gtgatggggc gtttattcgt gacgaggcta acattacgct
    11941 ggttgccgat tccccactgc gcgctttgct gatagatttg cctgtctagt tgtttttaca
    12001 gggagatgat gatgagtaaa aaatcgacca aaaagcgcca gctagcgaag cccgtggtgg
    12061 cgaaagaacc tgctcgcacc gccaaaaatt ttggctatga agagatgttg agcgagctgg
    12121 aagctatcgt cacggatgct gaaacgcgtt tagccgagga tgaagctacc gcgtaaagac
    12181 aggttgccgg atgcggcgta aacgccttat ccggcctaca aaacacaaca acatcaacaa
    12241 attaaacacc ccgtaggccg gataagatgc gccagcatcg catccggcat caggtacaaa
    12301 acacaacaac atcaacaagt tgagcacccc gtaggccgga taaggtgcgc cagcatcgca
    12361 tccggcacaa tactcaaaac ctggcgttta tctggccttg ctcgccataa tctcgataat
    12421 ctgccgatcc gtttgctgca tcgaatggct tgctaacgca cacaggttgg caatcgactg
    12481 ctcaacatca tgcgccacga tcccttcatt gccggtcacg gcggtatcat ccagcgccat
    12541 taacaccgct ttccacgcag ccgaagcact ggtcgaaacc ttcgcaccat cgcaaatcat
    12601 gccgctgaca tcgccgatca tactgctgat cgccatcgag atagtttcat aacgcccatc
    12661 caccagccat gccatcccgg cggcggcccc cattgctgcg gtcgttgcgg cacaaagtgc
    12721 agacaaacgc ggtaactggt tatggatata aattgcgctc aaatgagaaa gcatcagcgc
    12781 acgcgccagt cgttcatcat ccgctccgaa gtgttctgct acaaccacca caggcattgt
    12841 tgcggtgatc ccctggttac ccgagccgga gttactcata gccggaagcg tagcgccgcc
    12901 catacgcgca tcggatgccg cgctggtacg aatcacaatg gatgaagaga gatctttcgc
    12961 cagcaaaccg cgctcgcact gtttttccag cgtcgcgcca atatgcagcc cccacttacc
    13021 gctcaaacct tcctgcgata acgcacaatt tagcttcgcg gaatcgagaa taaagcggat
    13081 cgccgcaaat gggacttcat tgacgaactt caggatctca gccagcgtcg ttctggaaag
    13141 caccgtcagc ggcgactctt gctcgccctc tgccacacac gcctgctggg taaacaccac
    13201 accattgtgc gtctcaatat gcacaatgtt ggtatgcccg ccgacgatgg tgacacacgc
    13261 ccacttctca ccgttccaga ctttggcgcg tgagaagagg atttcattgc aaggttcctg
    13321 gatcttaaca gagactttcc ccgccgccag cagtgctttg gcatcggcaa ttgcctgcgc
    13381 agttgcgtct ttcagcactt ccagcccggc gttggcattt ccacctaacg cccccagcgc
    13441 cgccgcaatc ggcagcccca ccattcccgt gccgggaacg gtgatgccca gaccgttctt
    13501 catcagattt ggcgaaaccc aggcttctac acgttcaacc ggaccttcca gttctgccgc
    13561 agcaaccgcc gccgccagcg ccagtgaaat cggttcagta catcccagcg ccggttttac
    13621 ttcctcctga acggcgagga tgtaacgctg ccataacgga tttaaagtcg aatcaaacat
    13681 attaaaaacc ttaaaatttc aggtaaatca agaaaatgcc aggaacagag aaacacaaag
    13741 caacaaacca gtgacgataa tcaggtacag agacatccct ttgtatttgt gcaatgccgg
    13801 tactttgtaa accagccacg ccgggatcag gcaccctacc atgccgaaaa tcgggctaca
    13861 gatagaggtg aagctcaaca ccggtgcgtt cagtacgatg gcgctccagg ccagcaaaat
    13921 ggcgaaaatc atgatgccgc gctgaacaag attttcgtta atcttctcgg caggcatctt
    13981 gcgacgcagg atgttcatta cgatcccttg cgttgcttcg cgaaagccta aatagacgcc
    14041 aaagaatgca gtcatgacag caaagatatt gagaatgacg ctgaccactt tcacccacgc
    14101 tgcgccgtca ccgctaataa actgtgcggc aatcgccagc gtgaacgaca cggcgtagaa
    14161 aaagacggtg acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg ctttatgccg
    14221 cgccacttca attgattttt cccgcgagcg ataagagatc accatcgggc ttaacgtctg
    14281 gataaacaga atcgacgtca gggtaaacgg cagcgtaata atggcgtttt tcaccagcag
    14341 ccccagcggc ggtagcgaac cgacgttata cagatgccac attcccacca tcgacacgcc
    14401 cagcgcagcg actaccagca gcttggtcag caccatgtcg gtcgaaattt tgaataacaa
    14461 tttctcgccg cgtgaagaga tcgccaccag aatgcaaatc agtaccagac cataaaaggg
    14521 actgtctgac agcaaccctt ccgtcacgcc gaaggtatgc agataggaag cactatcgtt
    14581 ggtgatggcg gtggaataga cgaacatcca aatcaccagc attacgaaat agagcgcacc
    14641 taacaggatg ccccagtttt tacctaaata accgctaatg acactcgggt aatctttaca
    14701 ttctggtgat tctgccagcg tattaataaa caaccgctga aacagataca ttgccgggta
    14761 accaatcacc gatgagagca aaaataccca caatcccatc aaaccgacct gcaccgggag
    14821 aaaaacaatc cccgcgccaa tcgccatccc gatactcata atcacccagc cggtgtcagt
    14881 actgtcgaat ttgatcgctt ctcgccactc gctctcactc attccagcac gacccgccgg
    14941 ggtcgaagcg tctgcaatga cgcctttatt cgatgcaatt tccataattt ctcgctcaat
    15001 attttgtagg gtttattatt tttttccgag ccgcatcaag gcgatatgcg gtctacgttt
    15061 ttgcaggcga gttattagaa taaagaaatg atacgcgcga ggtcagagaa aatcttcaca
    15121 atcaagactt taaaataacg gagatggata agaattttct actaaattaa tcgcaagaga
    15181 atattcattc tctatttgcg gcagatcaca aaaaaaaggt gcacatttgt gcacccaagg
    15241 atgaaagctg acagcaatgt cagtcgcaga ccactttaat ggccagtcct ccgcgtgatg
    15301 tttcgcggta tttatcgttc atatctttgc cggtttcata catcgtctcg atcactttat
    15361 cgagtgaaac acgcggtgcc gaggtgcggc gcatcgccat ccgcgcggcg tttactgctt
    15421 tcacggcatt aatggcatta cgttcaatgc acgggatttg tacctgtccg gcaaccggat
    15481 cgcaggtcag cccaaggtta tgctccatcg cgatttccgc cgcattgcat acctgcgccg
    15541 gactgccgcc cagtagttca gttaaccctg ccgctgccat tgaacaggcc acgccaatct
    15601 ccccctgaca gccgacctcc gcgccagaga tggaggcgtt cattttatac agcgcgccaa
    15661 tagcccccgc tgccagaaaa tagcgggcaa ttgaccgctc gtttaccgga cgacggaact
    15721 tatcgtaata agccagcact gccggaataa tgccgcacgc accgttagtc ggtgccgtta
    15781 ccacgcgccc gccagctgcg ttttcttcac taaccgccag cgcgtacatg ttgatccagt
    15841 cgatgacatt catcggatcg ttagagatgt tatcgctaga aaccagctga cgacgcagcg
    15901 ctacggcacg acgcggcaca ttgagcggac caggcagcac gccttcagtg ttcatcccgc
    15961 gttcaatacc gtcatgcatc acttgccaga tacgggcaaa ccctgcgtca atttccgctt
    16021 tgctgcgcag cgccagctcg ttgtgcatca tcaggccaga tatagacagg ccgttgtaat
    16081 cacacatttt cagcagttca cctgctgagt ggaaatcgta aggtacggac gtttcgacat
    16141 cgtgcgacag gccgaagtgt tcttcttcga caataaaccc gccgccgaca gagtaatagg
    16201 ttttacttaa tagcgcttcc tgtcccttcc aggcagtgat ccgcattccg ttctcatggc
    16261 gaggcaacat ttcgggatgg aagataatgt tctttgctac aggaaaatca acaatatgcg
    16321 caccagatgc cactggcagc cgcccgctgc gcgttactaa ctctataaat gcagggatct
    16381 catcaatgac aacatcctgc ggactgtttc ctgccagtcc catgataatg gcgacatccg
    16441 tggcatggcc tttgcccgtc agtgacaacg acccgtacag atcgaccaca atatggctcg
    16501 tcgcggttaa taagccgcta ctttccagcc gatcaataaa actttttccg gcattcattg
    16561 gccccacggt atgcgaactg gagggaccaa tcccaatttt gaaaatatcg aatgcactaa
    16621 tcatatccac accctcggat tgccgttcag tgaagtggag cggaacgacc ttgcgaccgt
    16681 cccgctcacg aggctttacg cactacgtac tgcgatagct tcaatttcca gcttcacatc
    16741 tttcggcaaa cgcgcaacct gcacacagct ccgggtcggg taggtcgcct gatgctcatc
    16801 gaagaactgc ttatagactt cattgatggt ggcaaaatca ttcagatcgg tgatgaacac
    16861 ggtcatcttg atgatatcgc ccacgctcag cccggcagca accacgatcg ctttgacgtt
    16921 ttcgaggctt aaacgcgcct gatcttgcac atcagccggg atctcaccgg tctgcgggca
    16981 aaccggaatt tgcccggagg tgaagaccat tctgcctaaa tcaacgccct gaacataagg
    17041 gccgattgcg cctagggcac gttgcgtttc gataatcttt ttcatacatc ctccggcgtc
    17101 agagcgcctg ggtaaaagta cgtgaaataa catcctgttg ctgttcacgg gtcagtgcgt
    17161 tgaagcgcac ggcgtagcca gagacacgga ttgtcaggtt aggatatttt tccgggtgct
    17221 cgatggcatc cagcagcatt tcccgattca ttacgttgac gttgaggtgt tgaccgcctt
    17281 cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc gacaaggttg gttttacgta
    17341 ctggatcttc tttgcccagc gccgcaggaa cgattgagaa ggtgtacgag atcccatctt
    17401 tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga ggccacggca cctttgcggt
    17461 cacgaccatg catcgggtta gcgcccggcg cgaacggtgt tccggcgcga cgaccgtccg
    17521 gcgtattacc ggttttctgg ccgtacacca cgttagaagt gatagtcaga atcgactggg
    17581 tagggacggc gttgcgatag gttggcagcg ctttaatttt cttcataaag cgttcaacca
    17641 ggtcgcaggc aatgctgtct acgcgctcgt cgttgttgcc gtactgcgga tattcaccgt
    17701 cgatttcaaa gtccaccgcc aggccgtttt cgtcacggat tggtttcacg cgggcatatt
    17761 tgatggcaga cagggagtcc gtcgccaccg acaggcccgc gatgccgcat gccatagtgc
    17821 gatagacatc acgatcgtgc agcgccatca gcgaagcttc gtagctgtac ttgtcgtgca
    17881 tgtagtgaat gatattcagc gcgctgatgt actgcaccgc cagccagtcc atgaagtgat
    17941 cgaggctgtc catcactttg tcgtaatcca gcacgtcgtc catcagcggt gctgttttcg
    18001 gcccgacctg aatcttcagc ttctcgtcca ccccgccgtt aattgcgtag agcagcgttt
    18061 tcgccaggtt agcgcgtgca ccaaagaact gcatttgctt accaatcacc attgggctga
    18121 cgcagcaggc aatcgcgtaa tcgtcgctgt tgaagtcagt acgcatcaga tcgtcatttt
    18181 catactgcaa ggaagaggtg acgatcgaca cctgcgcggc atattttttg aaggcaatcg
    18241 gtaattcttc cgaccaaaga atggtcaggt taggttccgg tgccggcccc atagtgtgca
    18301 ggtgtgcaaa tagcggaagg agtttttggt caccagcgta cgaccgtcca gccccatccc
    18361 gccgatcact tccgtcgccc agattgggtc gccggagaac agcgaatcaa attccggtgt
    18421 acgcaggaag cgtaccatac ggatcttcat gatgaagtga tcgatcagtt cctgtgcctg
    18481 ctgctcattg aggacgccag ctttaaagtc gcgctcaatg tagatatcca ggaacgatgc
    18541 cgtgcgcccc agcgacatcg cgccgccatt ttgcgatttc actgccgcca gataagcgaa
    18601 gtagagccac tgcaccgctt cctgtgcatt ctgcgccggg cgagagatat caaagccata
    18661 tttcgccgcc atttcctgaa tctgcaacag cgcatgacga tgctctgcca gctcctcacg
    18721 cagacggatg gtggcttcca gatcctcgcc tttttccaga cgagactgga gatcggcaaa
    18781 ttgcagttcg cgttcacgca ccagataact gatgccatac agcgctacgc ggcgatagtc
    18841 accgataatg cgcccacgac catagccatc tggtaaaccg gtcagcacgc cagatttacg
    18901 gcagcgcagc atatccggtg agtaaacatc aaatacgccc tggttatggg ttttacgcag
    18961 atcggtaaac agatattcaa attcactgtc catttcacgg ccataggcgt ggaatgaact
    19021 tttaatcata ttaatgccac cgaacgggtg tagcgcacgt ttcaacggcg catccgtttg
    19081 caggccaaca attttttcca gcggctggtt aatatatccc gcatcatgag cggtaattgt
    19141 ggtggcaata ttggtatcga aatcaaccgg cgcgtgggtt gcgttttcga tacggatgcc
    19201 ttccattact ttttcccaca attccgtggt ggcaggcgtc gcttcggcga gaaaagattc
    19261 atcgccttca tacggtgtat agttatgttg aataaaatcg cggacattaa tttcgctttt
    19321 ccagtccgta cctttaaagc caagccatgc gtcagcgtac agcttatcgc tggtatcaat
    19381 atctaccttc atgaaaaata atctctctac aatacttcaa ctaaattatg caaattctgc
    19441 gggcgcgtta actttaccta aatgaatggc atccaaagca atcatttttt cttcgttagt
    19501 cggaataacg acgcaaatga catgcgcatt ttcactggaa acaattcgct cgccaaaaga
    19561 gttagatcga ttattcattt ctgtatcaat ctctacgcct aatacagcca aatgttccat
    19621 gaccagacga cgaattaaac ttgaattctc tcctattccg ccggtgaata taattccatc
    19681 cagccgacgt aatgaagctg catgtccggc aatatgacgg gcaattcggt gaacaaaggt
    19741 tttaattgcc agttgcgcgc gttcgtgacc ttcatgccag gctttttcca gaacacgtaa
    19801 atccgaagaa agaccggaaa tacctaataa tcccgactct ttattcacta cgcgttccag
    19861 gtcacccagg ctctggttgg tttggctggc gatccaggac atcgcaccaa agtcgacatc
    19921 gccactgcgg gtacccatca tcaagccttc cagcggcgtc attcccattg aggtgtcaac
    19981 actctgaccg ttgcgaactg cgcagattga cgcgccattg ccaagatgcg ccacaaccag
    20041 gccggagtca tcttccgcca gattcagcag cgaatgtgcg cgctgggaaa catagcggtg
    20101 cgacgtgccg tggaaaccat aacggcgtac acctaactct tcataatatt tccacggcag
    20161 gccgtataaa taagcttccg gagccatcgt ctggtggaaa ctggtatcaa atacggccac
    20221 ctgagttacg cccggaaata attgctgcgc cgattcaata ccacttaaat ttgcgtaatt
    20281 atgcaggggt gccagtggag aaacgcgacg gatattatca atgacttcat cggtaataat
    20341 ggcggactcg gtaaaaatac tgccgccgtg agcgatgcgg tggccaatta aggccacgct
    20401 gtcatttaaa ttccgttttt ccagttcaaa tgcaattgcc ttcaatgcac cttcgtagct
    20461 gtggagagcc agcggtgctg gctctccccc atttacggat aagaatgcat tttccgagtt
    20521 aataccgtcg gcaatacctg acattaatac ttcacagtcg ctggcatcga gtacggaaaa
    20581 cttaatcgaa gacgaaccac agttaataac caaaacaacc ggaaattcat tcatctcttt
    20641 tctcatcctg agttacggat taaaacagtt tgtatacgat gttcaggatg gtcagcagac
    20701 caatcacggt aacaaacacg ttatccagac gaccacggta tttcgccaga gacggcgctt
    20761 tacggatagc atacatcggc aacaggcaca gcagggatgc gataatcggt gcgcccatgg
    20821 cttcaatcag gtcgaggatg ttcgggttgg cgtaggcaac aacccaggtg gagcccatga
    20881 tgaagatcat gctgagagta ttcagtttac ccagcgacac tttggttttg tcacctttat
    20941 aaccgaactt cagaatcaga ccattcaagc cttccagcgt ccccagatag tgaccgaaga
    21001 aagatttgaa gatagccacg agtgcgatga tggaagccgc atattccagt gtaatcgcga
    21061 acgttgtttt cgtaccggtc atggacgcaa agtggttagc cagataagaa agcactggaa
    21121 tattctgcac tttggcttcc gccatgttgg ccggagacag agtaaacagg cagctaaagg
    21181 caaagaacat caccactgca accatcagca tgctggcacg agaaatgatt tgggaacatt
    21241 tacgttcggt gaagtcgcga ccgaagtctt tctcatactc ttcacgttta gaaaccacga
    21301 aggaagagac gattggcgag aagttaaagg agaaaaccat gatggaaatc cccagccaga
    21361 cagtgatcag gataccgtca tgaccggtta acgacagcga accgaggtca acctggtcga
    21421 taactgcaga gttccagtaa gggatcagcg acagagaaat cagcaccagg ctggcgataa
    21481 acggccatac caggtagctc atcactttaa ccatcagatc cttaccaaac cagatgacga
    21541 aagccatcag cagcaacagg aacagcgcca caaagccgcg attcagcggt gcaaatccga
    21601 gctggttttc ccagaacgtc ataaaggtat tggtaatagt aacgccataa atccacagca
    21661 gtgggcaaat cgcgaagaag tacaggaacg tgataaccac gccgccagtt ttaccaaaat
    21721 gctcttccac cgtttccgta atgttgccgg aagggttaga gccagaaaga cacagacgcg
    21781 ccagcgcccg gtggcaataa aatgcgatgg ggtatgccaa taccaacatc agaagaatcg
    21841 ggatcagtcc gccaaaacct gcgcggatag ggaagaacag caccccggcg ccgattgccg
    21901 taccaaacaa gcctaacgtc catgtggtat ctgatttacg ccaggacgat tgtttcgtct
    21961 ggctggatac aatgctatct gaagtactca tatcctatcc tcaacgaatt aattaagcgt
    22021 caacaaaacc ggtgatttga gagacgcgag aaagatcgat attgccgccg gaaataatac
    22081 tgacggtttt tctgttttga atatattggt ctaatttacc gcttaataat gcagcacatg
    22141 ccagagcgcc tgcgccttcg gtgacgactt tattgcgctg aattaaggca atcatactgt
    22201 ttctgatttc gtcttcgctg accagcacga tgtcatcgac taattcacga acgatttcgt
    22261 aagttaaatt acccgggcgg gagacatcac aaccatccgc cagggtgccg gtagttcggt
    22321 gcgtggttat ttctccggag tggaaagaag ccgccatgcc gtgaacgttt tctgactgta
    22381 cgccaataac acgaatggtc gggttaatag atttaattgc caccgcaata ccagcaatta
    22441 aaccaccacc accaattggc acaatcacgt tatcgacatc atagagatct tccataattt
    22501 ccagaccaat cgttccctgg ccagcaatca ctttcggatc atcgtaaggt gggataaaaa
    22561 tacgaccttc catttcgaca atttcgctca ctttagcgat agtgtcgttg aagttatcac
    22621 catgcagaac gacttctgcg gagtagtcgc acgttgccgc tactttggat tttggcgcac
    22681 cttttggcat caccacttta ccgtcgatac ccagcatcgc gcaggagagc gaaacccctt
    22741 gcgcatggtt gcccgcagaa caggccacca cgcctttgcg tttttccgca tcggtcagtg
    22801 aacttaattt attaaatgcg ccacgaattt taaatgaacc cgtacgctgc atattttcaa
    22861 acttcaggaa tatttcacct ttgcaacgtt cactaaaata gttggagcga ggcatgcctg
    22921 ttttataaat tcgcccagcc agtcgttgtt tcgcttcaat aatatcatca atagcaaccg
    22981 gcagatcgta tgtaatatgc attataaaac ctcttcgcct gaattaaata tgtaggtaac
    23041 cgtaaccgac acctgcaaaa cagacaggtg gattatttat gtttaaataa aaacaaataa
    23101 ctaaccaact tctattaatt gccttcgtct acacccatta taagatgaat actctttggc
    23161 taattccacc aaaaccgatg ctgctttttt aatacgataa tttttcgacc ataccgcggc
    23221 atattgtgcc acaggtaatg tttcttcaac cggaatagta ataaattgat tagaaccaaa
    23281 aggtgacgtc atatcacaag gaattacagt taagaaatca gcattgagaa caagattata
    23341 aattgtcacg actgagtcgg ttttaacgat gttttcaata ctgatgccat ttctttgtaa
    23401 cgtagtaagc agttcgctgt agtaccccat attagtttgt ggcaacaccc actgttcgtt
    23461 cttcaacgac tccagcgtgg tggtgccggt gcatgttcgg gacttactgg ctaccagcac
    23521 aaactcggac tcgaacagcg gctcaacatg taaatcctga agcttcattt ctgcacttaa
    23581 cgtaccaatc gcaaaatcca gtcgaccgtc gcggattgcc ggtaagaacg aagacagttg
    23641 cgcttcatac atagaaacct gcgctttcgg gaacacctct ttgaacttgt tgatcatccc
    23701 tgacataaaa gtaaaaccaa tcaatgaagg aaaaccaaat gagacttcca ccaccgcctc
    23761 agaagacata ccgcttatct cattaaccat atttttcatt tcacgggtaa tggattcgga
    23821 acgggagagt aacaattgac cggcaggtgt taatgttaca ccggtatttt tccgcaccac
    23881 taattccaca ccaaaataat cttcaatatc gttaatgatt ttactgacgg ccggttgagt
    23941 taaccctaat tcttttgcag ccgagccgat agagccactt ctaatgactt cctgaaagac
    24001 taccaggtgc tgcgttttcg gaagaagaat agtgctcata acgacctacg ttaattacct
    24061 cattgacggc atgaagtgta tcaaaatgaa ataaacagga tatgtgcgac cactcacaaa
    24121 ttaactttca ttactttcca aggtatcgtt attacaatta aataccttta aatatcaaca
    24181 agttaaagca taaaaatcag cataaaccct gatttctatc aaaaaaaatg acggggataa
    24241 ccatatttta tggcgataac atcattcgtt atgtggttaa tacaaaaaag gggctgagac
    24301 catatttcaa gcaattaccg gataattacc aggcaaaatt tgagttaaat ttaatgagca
    24361 agttctcaaa ttttattata aataaaccat tttcagcgat aaatactgcg gcacgtcaca
    24421 ttttttcacg ctatttgttg gagaacaaac atttatttta tcaatatttt aaaatttcga
    24481 atacaggtat tgatcatctc gaacaattga ttaacgtcaa ctttttctct tctgacagga
    24541 cgtcattttg tgaatgcaat cgttttccat aaattcttct cccctcatag gcgacgaata
    24601 gcattttgtg ttgaggatca caaaacgaat aattgctggt cgccgcgata aggtcagacg
    24661 aagtcaacaa gggggatttt cactgtgctt tacatcagcg taggacacag aatgttgctt
    24721 cgtttactgt aacgccgggt aaatgagcgt tttttgatag tgcgtaagtg tcgtgcgagg
    24781 aaaataagca tctattattg tttgtcatga cgacaaggca ggcatcaatg aaaggcttcc
    24841 caattgcaca tatttttcac ccttcaatcc cgccaatgca cgcagtggtt aacaatcaca
    24901 atagaaatat tgattattgg acggtaaaaa gaaagtttgc agaaattgtc tccaccaatg
    24961 acgttaataa aatttacagt ataagtaatg aactgcggag agtattatct gcaattactg
    25021 cattgaattt ctatcaaggc gatgttcctt ctgtcatgat ccgaatccaa ccggaaaata
    25081 tgagtccatt catcatagat atttctacag gagaacatga tgattatatc atacaaacat
    25141 tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc gtcaataaaa
    25201 aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc acccaaaaag
    25261 aagccatctt aacaccccct aggcacttta ataaaacctg cattacttca gattgttggc
    25321 aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta tttataaagg
    25381 ataaaataat gttccctgtt tcatcaattg
//
LOCUS       DAJTYG010000053        25215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTYG010000053 DAJTYG010000000
VERSION     DAJTYG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.053"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            4..522
                     /locus_tag="OO568_002341"
     CDS             4..522
                     /locus_tag="OO568_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6641673.1"
                     /translation="MVAAITCISQPKKTTLRDKAMVNYAFDYLRSPGSLPLTTAATEL
                     SAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGL
                     GVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYK
                     NAERLLAGLPAH"
     gene            complement(532..1959)
                     /locus_tag="OO568_002342"
     CDS             complement(532..1959)
                     /locus_tag="OO568_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6641674.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1956..2663)
                     /locus_tag="OO568_002343"
     CDS             complement(1956..2663)
                     /locus_tag="OO568_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6641675.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWILSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2821..3804
                     /locus_tag="OO568_002344"
     CDS             2821..3804
                     /locus_tag="OO568_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6641676.1"
                     /translation="MIIIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAID
                     SPDDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFA
                     SNALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFW
                     YKQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNA
                     GEGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECN
                     DATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(3874..4356)
                     /locus_tag="OO568_002345"
     CDS             complement(3874..4356)
                     /locus_tag="OO568_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6641677.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTELDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            4591..7173
                     /gene="leuS"
                     /locus_tag="OO568_002346"
     CDS             4591..7173
                     /gene="leuS"
                     /locus_tag="OO568_002346"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6641678.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATLEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7188..7769
                     /gene="lptE"
                     /locus_tag="OO568_002347"
     CDS             7188..7769
                     /gene="lptE"
                     /locus_tag="OO568_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6641679.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            7769..8800
                     /gene="holA"
                     /locus_tag="OO568_002348"
     CDS             7769..8800
                     /gene="holA"
                     /locus_tag="OO568_002348"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6641680.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8802..9443
                     /gene="nadD"
                     /locus_tag="OO568_002349"
     CDS             8802..9443
                     /gene="nadD"
                     /locus_tag="OO568_002349"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6641681.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9467..10078
                     /locus_tag="OO568_002350"
     CDS             9467..10078
                     /locus_tag="OO568_002350"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR6641682.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10338..10655
                     /gene="rsfS"
                     /locus_tag="OO568_002351"
                     /gene_synonym="rsfA"
     CDS             10338..10655
                     /gene="rsfS"
                     /locus_tag="OO568_002351"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6641683.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10659..11126
                     /gene="rlmH"
                     /locus_tag="OO568_002352"
     CDS             10659..11126
                     /gene="rlmH"
                     /locus_tag="OO568_002352"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6641684.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVILDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11157..13058
                     /gene="mrdA"
                     /locus_tag="OO568_002353"
     CDS             11157..13058
                     /gene="mrdA"
                     /locus_tag="OO568_002353"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6641685.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13061..14173
                     /gene="mrdB"
                     /locus_tag="OO568_002354"
                     /gene_synonym="rodA"
     CDS             13061..14173
                     /gene="mrdB"
                     /locus_tag="OO568_002354"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6641686.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14184..15272
                     /gene="rlpA"
                     /locus_tag="OO568_002355"
     CDS             14184..15272
                     /gene="rlpA"
                     /locus_tag="OO568_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6641687.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAQTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15411..16622
                     /gene="dacA"
                     /locus_tag="OO568_002356"
     CDS             15411..16622
                     /gene="dacA"
                     /locus_tag="OO568_002356"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6641688.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16733..16996
                     /locus_tag="OO568_002357"
     CDS             16733..16996
                     /locus_tag="OO568_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6641689.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17097..17738
                     /gene="lipB"
                     /locus_tag="OO568_002358"
     CDS             17097..17738
                     /gene="lipB"
                     /locus_tag="OO568_002358"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6641690.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17997..18950
                     /locus_tag="OO568_002359"
     CDS             17997..18950
                     /locus_tag="OO568_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6641691.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQSVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCRP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19159..20124
                     /gene="lipA"
                     /locus_tag="OO568_002360"
     CDS             19159..20124
                     /gene="lipA"
                     /locus_tag="OO568_002360"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6641692.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20225..20428)
                     /gene="tatE"
                     /locus_tag="OO568_002361"
     CDS             complement(20225..20428)
                     /gene="tatE"
                     /locus_tag="OO568_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6641693.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20557..21345)
                     /locus_tag="OO568_002362"
     CDS             complement(20557..21345)
                     /locus_tag="OO568_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6641694.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            21438..21821
                     /gene="crcB"
                     /locus_tag="OO568_002363"
     CDS             21438..21821
                     /gene="crcB"
                     /locus_tag="OO568_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6641695.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21875..22084)
                     /gene="cspE"
                     /locus_tag="OO568_002364"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21875..22084)
                     /gene="cspE"
                     /locus_tag="OO568_002364"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6641696.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22259..22819)
                     /gene="pagP"
                     /locus_tag="OO568_002365"
     CDS             complement(22259..22819)
                     /gene="pagP"
                     /locus_tag="OO568_002365"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6641697.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23404..24789
                     /gene="dcuC"
                     /locus_tag="OO568_002366"
     CDS             23404..24789
                     /gene="dcuC"
                     /locus_tag="OO568_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6641698.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24830..25171)
                     /locus_tag="OO568_002367"
                     /pseudo
     CDS             complement(24830..25171)
                     /locus_tag="OO568_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT         6424 a         6281 c         6214 g         6296 t
ORIGIN      
        1 tttatagttg cagcaattac ctgtattagc caacccaaga aaacgacgtt gcgtgataaa
       61 gccatggtga attatgcctt tgattattta agatcaccag gcagtctacc gttaaccacg
      121 gcagccacgg agctttccgc gattcatggt cactcaacat cgcaatatcg ccttggcgaa
      181 ttttatcttc atggtagcga cggtaaacca ctggattata cacaggcgag atactggtat
      241 gagcaatcag cagaacagga aaatccgcgc gcacaaagca aactggggtg gatctacctg
      301 aaaggcctgg gagtcaaacc cgatacccgt aaagcaattc tctggtataa ggaagcagct
      361 gaacaagggt atgctcatgc tcaatatact ttgggtttga tctacagaaa tggcactggt
      421 attaatgtta accattatga atctcaaaaa tggttaaaac tggccgccaa acaacattac
      481 aaaaatgcgg aaagattact tgccggactt cccgcacatt aacgtcgact gtcacccgaa
      541 gagatggagt attttcccta tcaaaccggc aaagataaaa atgtagaaaa taattttgat
      601 gacgcccaga cagcccagcc cgccatcgtc aacgggcatt ggctgcggtt gcggctccgg
      661 ctcgacggtt tcctgaccgg taaaattcgc cgcctgaatt aataatcgac gaataattaa
      721 tgcttccagt ggacaacgat cttctgcatc aaggcggctc acaatcggca ttaatgattc
      781 ttctgtcggt tggctggcct gcactaattg tttggcaact aaatataatg gccataaggg
      841 atgttctgta tcacgcgtga gtgagatccg cggcttacgt cctttgtgca aaaccatctc
      901 gaccagtgaa cgccccagcg cagagagttt ttcatcgctg agtatttcgt ataaatgaaa
      961 acgcgtacgg gcgctgtcgc cggggacata aagtagcggt tttccctgat cgttaaccag
     1021 atcacgcgct tcaatcatca tcaccactaa tgggtaataa tcgacccgat gctgacggca
     1081 ccattgcaac attaaagcat ccgcctgggt ggagggatac tcgcgccagt agtaacaaag
     1141 ttctgccagc aggctttcgc cttctccaca atagacgcgt tgtgcgtatt cataataacg
     1201 cgcatcttca ttatcaggtt cagcttcccg ccacgaccgg gcgtaatcca ggagttcggg
     1261 gattcccagg cggcttgcgc tataccagcg gagtaaattt ctgtgcagtt ttgcatcatc
     1321 ggggataatc actgcgcgat gttgcgctaa atacaatgtc agccattctg gatgcgatga
     1381 ccaaatccga gcgaacattt ggtaaaaatc gatggtgtca ttttgcacgg caacgggcag
     1441 atgcagaatg gtctggaagt taaaaacatt ccccttcgca atggcatata aaaaggattc
     1501 cagttcttct tcgtctattt ccccgacgtt ttcctcctgc aaccaccgca atctttctgc
     1561 taataacacc acgcactcga aggataaatg ggcagtgttc atggcgattg tgcacagcga
     1621 ccagcgtaat tcatcaatct cttccatcga gcaataattt aattgctgaa tgaatcgctg
     1681 ccaggcggag ggcagaaaac gttcgctatc agaggcaagt aacgcgcgaa aggcgagcag
     1741 aatttcatgt tctgctacct catattcttc tggttgccaa acagatttag caggcgactg
     1801 cgcaatccgt agcgcctcct catacgcctg acgaagttgt ttaaaaccct gcggatcggt
     1861 ttctggatga aaggacggta acagcgccag ataagcgcgg cggataatat cgacgtcagt
     1921 cgtttcctct atatcgagga tcttccagca atttttcata agtcgctcat atccttaagc
     1981 gtttccgggc atgtggggta gtcagccagt ggttgccagg acaaggtagg gtaatacgca
     2041 tcatcactgg caaataattg ataaaacata tcattgccgg aatcgtcgta ttctttacgc
     2101 gctaagaatt cgcgtaataa aggcagatga tcatcttcgg gagactgcca atattgccga
     2161 ccaaaggtat aaccggcaaa gtattgccgc cagttactat aataacgtag agcccgtaga
     2221 tgaatacgcg acaggatcca tagactttct tcttcactca accagttatt aagtacgccc
     2281 attcgactta aaaaacccat ccggacataa tcccatgctt taattcctcc ctcaccacag
     2341 cattcggctg tgctggcgac aaattgcgca taagcctgac cttgctcatt taattcagca
     2401 agatagtcac gccattcaca tggagaataa cgaaaccagc ggtgataaaa ccctgccaac
     2461 cgagcggcat gaccgttctc gatcattcgg tgaattgtgc gcagcaggtc atcacggtca
     2521 tcaattcccc atgaggaaga aaggtcaaaa aagcatgtac ctaaatacat tttgggggat
     2581 gaatgcgaag gtaaacagca ttcattgcgt atttccatcg gagtggaaag tgcaaacaat
     2641 attttttgcg accccatgtc catataattt atccttaaac attcatttaa tgaatgcaaa
     2701 ctacatttcg agactcagtt atttatactt ttctgacgca acataatacg cataaaataa
     2761 ggcttacgca aaaatggaac acataaaaat ataatatatt ttatctacaa ccataaggaa
     2821 atgataataa tttttacgtc aagttgctgc gataatttat caatagatga gattatcgaa
     2881 cgtgctgaaa aaggcgattg cgaggctcaa tatattgttg ggttttatta taatcgcgat
     2941 agcgcaattg actctccgga cgacgaaaag gccttttact ggctgaagct ggccgctgag
     3001 caaggtcatt gtgaagcaca gtattcctta gggcaaaagt ataccgagga taaaagccgt
     3061 cataaagata atgagcaagc catcttctgg ctgaaaaaag ctgccctaca aggccatact
     3121 ttcgcttcca acgcccttgg ctggatactg gatcgtggag aagaccccaa ctataaagaa
     3181 gcggttacct ggtatcagat agccgcggag agcggaatgt cttatgcgca aaataatctt
     3241 ggatggatgt acagaaatgg caacggagtc gcacaagact atacgctggc atttttctgg
     3301 tacaaacaag ctgcattaca aggccatagt tacgcacaag acaatctggc cgatctttat
     3361 aaagacggag aaggcgttgc tcaaaacaag acactcgccg cattctggta tttgaaaagc
     3421 gcacagcagg gtaatcggca cgcccagttt caaattgcat gggattataa cgctggcgaa
     3481 ggggtggacc aggactataa gcaagcgatg tactggtatc tgaaggctgc cgctcaggaa
     3541 agcgtcggcg cttacgtcaa catcggttat atgtataaac acggacaagg cgttgagaaa
     3601 gattatcagg ctgcctttga atggtttacg aaagccgctg aatgcaatga cgccactgcc
     3661 tggtataacc tagccattat gtatcactac ggagaaggaa gacctgtcga tctccgacag
     3721 gctctcgacc tgtatcgtaa agttcagtca tccggaacca gagatgtcag tcaggaaatt
     3781 cgtgagactg aagctttact gtagaaataa aaacaagggc cggggaattt cattctgccc
     3841 atattctctt tattgctatg cgaaatcaga ctattacggc tcaaacgggc gtctctggaa
     3901 ctggtcataa ccacatttcg ggcatagcgt cagcacttcc ggtgtgtaga tcgggagatg
     3961 gaagtgacat ttctcgcaga ccagatttcc cagcccgacc acttctccgc tgtgataaac
     4021 cccatgatga ttgaggtcct ggaaaacttc gcgccattca agctgcgttt tatcggtgat
     4081 gtctgccagc tcctgccaca agctttcttt aatcacccgc ataaagacgc tgtcagattc
     4141 ttctttcagg ctctcttcat agctcatggc gaactcttcc aggtcacgtc tgacagctcg
     4201 cgtcagctca tcgagctcgg ttcgcgttaa ctcccctgtt tttattacgc gctcgcgcgc
     4261 ctgttccacc agtgcgtcga tatcacgttc gccattgcgc aggcgttcgt tcagtgacgc
     4321 gaccagttca cggtaatatt gagcaacctt gttcatcgtt tcatctcccg ggtcgttaac
     4381 tgactataat aatagacgtt ctttgttcct tgctttgcta atacgcccac aaactctgta
     4441 aattaatgta cgaataatcc gggcatcatt tcaacgtcga gtgcgcgaaa ggctgttttg
     4501 acgcggacgt ttgggctatg ctatgcggat ctgaaaaacc acctcaacgc tacatttgta
     4561 gccgtattga aaacaggacc actggctgcc atgcaagagc aataccgccc ggaagagata
     4621 gaatccaaag tacagcttca ttgggatgag aagcgcacat ttgaagtaac cgaagacgag
     4681 agcaaagaga agtattactg cctgtctatg cttccctatc cttctggtcg actacacatg
     4741 ggccacgtac gtaactacac catcggtgac gtgatcgccc gttaccagcg catgctgggc
     4801 aaaaacgttc tgcagccgat cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg
     4861 gtgaaaaaca acaccgctcc ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac
     4921 cagctcaaaa tgctgggctt tggttatgac tggagccgcg agctggcaac ctgtacgccg
     4981 gaatactacc gttgggaaca gaaattcttc accgagctgt ataaaaaagg cctggtatat
     5041 aaaaagactt ctgcggtcaa ctggtgcccg aacgaccaga ccgtactggc gaacgaacaa
     5101 gttatcgacg gctgctgctg gcgctgcgat accaaagttg aacgtaaaga gatcccgcag
     5161 tggtttatca aaatcactgc ttacgctgac gagctgctca acgatctgga taaactggat
     5221 cactggccag acaccgttaa aaccatgcag cgtaactgga tcggtcgttc cgaaggcgtg
     5281 gagatcacct tcaacgttaa cgactatgac aacacgctga ccgtttacac tacccgcccg
     5341 gacaccttta tgggttgtac ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa
     5401 gcggcggaaa ataatcctga actggcggcc tttattgacg aatgccgtaa caccaaagtt
     5461 gccgaagctg aaatggcgac gctggagaaa aaaggcgtcg atactggctt taaagctgtt
     5521 cacccattaa cgggcgaaga aattcccgtt tgggcagcaa acttcgtatt gatggagtac
     5581 ggcacgggcg cagttatggc ggttccgggt cacgaccagc gcgactacga gtttgcctct
     5641 aaatacggcc tgaacatcaa accggttatc ctggcggctg acggctctga gccagatctt
     5701 tctcagcaag ccctgactga aaaaggcgtg ctgttcaact ctggcgagtt caacggtctt
     5761 gaccatgaag cggccttcaa cgccatcgcc gataaactga ctgcgatggg cgttggcgag
     5821 cgtaaagtga actaccgcct gcgcgactgg ggtgtttctc gtcagcgtta ctggggcgcg
     5881 ccgattccga tggtgacgct ggaagacggt accgtaatgc caaccccgga cgaccagctg
     5941 ccggtgatcc tgccggaaga tgtggtgatg gacggcatta ccagcccgat taaagcagat
     6001 ccggagtggg caaaaactac cgttaacggt atgccagcgc tgcgtgaaac cgacactttc
     6061 gacaccttta tggagtcctc ctggtactat gcgcgctaca cttgcccaga gtacaaagaa
     6121 ggtatgctgg attccgaagc ggctaactac tggctgccgg tggatatcta cattggtggt
     6181 atcgaacacg ccattatgca cctgctctac ttccgcttct tccacaaact gatgcgtgat
     6241 gcaggcatgg tgaactctga cgaaccagcg aaacagttgc tgtgtcaggg tatggtgctg
     6301 gcagatgcct tctactatgt tggcgaaaac ggcgaacgta actgggtttc cccggttgat
     6361 gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag cgaaagatgc ggcaggccat
     6421 gaactggttt ataccggcat gagcaaaatg tccaagtcga agaacaacgg tatcgacccg
     6481 caggtgatgg ttgaacgtta cggcgcggac accgttcgtc tgtttatgat gtttgcttct
     6541 ccggctgata tgactctcga atggcaggaa tccggtgtgg aaggggctaa ccgcttcctg
     6601 aaacgtgtct ggaaactggt gtacgagcac acagcaaaag gtgatgttgc ggcactgaac
     6661 gttgatgcgc tgactgaaga tcagaaagcg ctgcgtcgcg atgtgcataa aactatcgct
     6721 aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca ccgcaattgc ggcgattatg
     6781 gagctgatga acaaactggc gaaagcaccg accgatggcg agcaggatcg cgctctgatg
     6841 caggaagcac tgctggccgt tgtccgtatg ctcaacccgt tcaccccgca catctgcttc
     6901 acgctgtggc aggaactcaa aggcgaaggc gatatcgaca acgcgccgtg gccggttgct
     6961 gacgaaaaag cgatggtgga agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc
     7021 cgtgccaaaa tcaccgttcc ggtggacgca acggaagaac aggttcgcga acgtgctggc
     7081 caggaacatc tggtagcaaa atatcttgat ggcgttactg tacgtaaagt gatttacgta
     7141 ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg aggaagcgtg cgatatctgg
     7201 caacattgtt gttatctctg gcggtgttaa tcaccgccgg gtgtggctgg catctgcgtg
     7261 acaccacgca ggttccttcc actatgaagg tcatgatcct ggactcaggc gatccgaacg
     7321 ggccattaag ccgtgcggtg cgtaaccagt tacgtctgaa tggtgtcgag ttgcttgata
     7381 aagaaaccac gcgtaaggac gttccatccc tgcgtttggg taaagtgagc atcgcgaaag
     7441 ataccgcatc ggtattccgt aacggtcaaa cagcagagta tcagatgatc atgacggtga
     7501 atgcgaccgt gttgatcccc ggccgtgata tctacccaat tagcgccaaa gtcttccgtt
     7561 cattcttcga taacccgcaa atggcgttag cgaaagataa cgaacaagac atgatcgtaa
     7621 aagagatgta cgaccgtgct gccgaacagc tgattcgtaa gctgccaagc atccgtgctg
     7681 cggatattcg ttccgacgaa gaacagacgt cgaccacaac ggatactccg gcaacgcctg
     7741 cgcgcgtctc caccatgctg ggtaactgat gattcggttg tacccggaac aactccgcgc
     7801 gcagctcaat gaagggctgc gcgcggcgta tcttttactt ggtaacgatc ctctgttatt
     7861 gcaggaaagc caggacgctg ttcgccaggt agctgcggca caaggattcg aagaacacca
     7921 cactttttcc attgatccca acactgactg gaatgcgatc ttttcgttat gccaggctat
     7981 gagtctgttt gccagtcgac aaacgctatt gctgttgtta ccagaaaacg gaccgaatgc
     8041 ggcgatcaat gaacaacttc tcacactcac cggacttctg catgacgacc tgctgttgat
     8101 cgtccgcggt aataaattaa gcaaagcgca agaaaatgcc gcctggttta ctgctcttgc
     8161 gaatcgcagc gtgcaggtga cctgtcagac accggagcag gctcagcttc cccgctgggt
     8221 tgctgtgcgc gcaaaacagc tcaacttaga actggatgac gcggcgaatc aggtgctctg
     8281 ctactgttat gaaggtaacc tgctggcgct ggctcaggca ctggagcgtt tatcgctgct
     8341 ctggccagac ggcaaactga cattaccgcg cgttgaacag gcggtgaatg atgccgcgca
     8401 tttcacccct tttcattggg ttgatgcttt gttgatggga aaaagtaagc gcgcgttgca
     8461 tattcttcag caactgcgtc tggaaggcag cgagccggtt attttgttgc gcacattaca
     8521 acgtgaactg ttgttactgg ttaacctgaa acgccagtct gcccatacgc cactgcgtgc
     8581 gttgtttgat aagcatcggg tatggcagaa ccgccggggc atgatgggcg aggcgttaaa
     8641 tcgcttaagt cagccgcagt tacgtcaggc cgtgcaactc ctgacacgaa cggaactcac
     8701 cctcaaacaa gattacggtc agtcagtgtg ggcagagctg gaagggttat ctcttctgtt
     8761 gtgccataaa cccctggcgg acgtatttat cgacggttga tatgaaatct ttacaggctc
     8821 tgtttggcgg cacctttgat ccggtgcact atggtcatct aaaacccgtg gaaacgctgg
     8881 cgaatttgat tggtctgacg cgggtcacaa tcatccctaa taatgttcct ccgcatcgtc
     8941 cccagccgga agcgaacagc gtgcagcgta aacacatgct tgaactggcg attgccgaca
     9001 agccattatt tactcttgat gaacgcgagc taaagcgcaa tgccccctct tacactgcac
     9061 aaacactgaa agagtggcgg caggaacaag gaccggacgt gccgctggcg tttattattg
     9121 gtcaggattc attgctgacc tttccgacct ggtacgaata cgaaacgata ctcgacaatg
     9181 cacatttgat cgtctgtcgg cgtccaggtt acccacttga aatggcgcaa ccgcaatacc
     9241 agcaatggct ggaagatcat ttgacacata acccggaaga tcttcacctt cagcctgccg
     9301 gtaaaattta tctggctgaa acgccgtggt ttaacatctc ggcgaccatc atccgcgaac
     9361 gtttgcaaaa cggtgaatcg tgtgaggatt tattgccgga accggtattg acttacatta
     9421 accaacaagg cttgtatcgc tgatacctgt cgttacggag ggcatgatgc gactgtggtt
     9481 aattcgtcat ggcgaaacac aagcgaatgt cgatggtctg tacagcggtc atgcgcccac
     9541 acccctgacc gcacgcggta ttgagcaagc gcaaaatctg catacactgc tagatgatgt
     9601 ttcctttgac ctggttttat gcagtgaact ggaacgggca cagcataccg cgcgactggt
     9661 tctcagtgac cgccagctcc ccgttcatat catccctgaa ctcaacgaaa tgttttttgg
     9721 cgactgggag atgcgacatc atcgcgacct catgcaagaa gatgccgaaa actatagcgc
     9781 gtggtgcaat gactggcagc atgctatccc cacgaacggt gaaggatttc aggcattttc
     9841 gcaacgtgtg gaacgcttta tcgcaaggct tagtgaatat cagcactatc agaatatttt
     9901 aatcgtcagc catcagggcg tactgagtct gttaattgct cgtttaattg gcatgcctgc
     9961 cgagtctatg tggcattttc gcgttgacca ggggtgttgg agcgccattg atatcaacca
    10021 aaaattcgcg acgctacgcg tcctcaatag ccgtgccatc ggggtcgaga atgcatgact
    10081 tttctgtttt tttacgggta agccgcaacg accattgaca gccccgggca ggctgatatt
    10141 ctccgcagcc agactttttc cgccagacac gactttgtag aaattgtttt acaaaaatgg
    10201 cgatgcaatc tgcggcgcgg ggtgggatga tagcccactt tcgaaagccg attcggcgac
    10261 aattgtcccg aaatcgcctc tggttcaggt atactgacag accattttta tctatttgat
    10321 tcacccaggg ggaaaacttg cagggtaaag cactccagga ttttgttatc gacaaaattg
    10381 atgacctcaa aggtcaggac atcatcgcct tagacgttca aggcaaatcc agcatcaccg
    10441 actgcatgat catctgtacg ggtacgtcca gccgtcatgt tatgtccatt gctgaccacg
    10501 ttgtgcagga gtctcgcgca gcgggcctgt taccgctcgg cgtagaaggt gaaaacagcg
    10561 ccgactggat tgtcgtggat ttgggcgatg tgattgtcca tgtcatgcag gaagagagcc
    10621 gtcgcctgta tgaactggaa aaactctgga gttaatgcgt gaagctgcaa cttgtcgccg
    10681 tgggaacgaa aatgccggac tgggtacaaa ccggttttac cgagtacctg cgtcgttttc
    10741 cgaaagatat gcccttcgag ctgattgaaa ttccggccgg caaacgcggc aagaatgcgg
    10801 acatcaagcg catactcgac aaagagggtg agcagatgtt ggcggccgca ggcaaaaacc
    10861 gcattgtcat cctcgatatt ccaggcaagc cctgggatac gccgcagtta gccgctgagc
    10921 tggaacgctg gaagctggat ggtcgcgacg taagtctact gattggcggg cctgaagggt
    10981 tgtcgcctgc ctgtaaagcg gcggctgagc agagctggtc gctgtcggcg cttaccctcc
    11041 cccatccgct ggttcgcgtg ctggtcgcag agagtctgta ccgggcgtgg agcatcacca
    11101 ccaaccatcc ttatcaccgt gagtgataag ggagctttga gtagaaaacg cagcggatga
    11161 aactacagaa ctcttttcgc gactatacgg cagagtccgc gctgtttgtg cgccgggcgc
    11221 tggtcgcctt tttggggatt ttgctgctga ccggcgtgct tatcgccaac ctgtataatc
    11281 tgcaaattgt tcgctttacc gactaccaga cccgctctaa tgaaaaccgc attaagctgg
    11341 tgcctatcgc gcccagccgc ggcattatct acgaccgtaa cggtatccct ctggccctca
    11401 accgcactat ctaccagata gaaatgatgc cggagaaagt cgataacgtg cagcaaacgc
    11461 tggacgcttt gcgcagcgtg gtagatctga ccgatgacga tattgctgca ttccgaaaag
    11521 agcgcgcacg ttcacaccgt ttcacctcta ttccggtgaa aactaacctg accgaagtac
    11581 aagtagctcg ctttgccgtc aatcagtacc gttttccggg tgtcgaagtt aaaggctata
    11641 aacgtcgtta ctatccttac ggttcggcgt tgacccacgt catcggatat gtgtcgaaaa
    11701 tcaacgataa agacgtcgaa cgcctgaata atgacggcaa actggccaac tatgcggcaa
    11761 cgcatgatat tggtaagctg ggcattgagc gttactatga agacgtgctg cacggtcaga
    11821 ccggttatga agaggttgaa gttaacaacc gtgggcgtgt tatccgccag ttaaaagaag
    11881 taccaccgca agccggacac gatatttacc tgacgctgga tctcaaactc cagcaatata
    11941 ttgaaacgct gctggcgggt agccgcgcag ctgtggttgt taccgatccg cgtacaggtg
    12001 gggtgctggc gctggtttcc acgcctagtt atgacccaaa cctgtttgtt gacggtatct
    12061 ccagcaaaga ttattccgcc ttgctgaacg acccgaatac accgctggtg aaccgcgcca
    12121 cacagggggt ttatcctccc gcgtctacag ttaaacccta tgtggcggtt tcggcattaa
    12181 gcgccggggt gatcacgcgc aatacgacgc tgtttgaccc aggctggtgg caactaccag
    12241 ggtcggaaaa acgttatcgt gactggaaaa aatggggcca cgggcgtctg aatgtcacaa
    12301 gatcgctgga agaatctgcg gataccttct tctatcaggt ggcctacgat atggggatcg
    12361 atcgcctctc cgaatggatg ggtaaattcg gttatggtca ttacaccggt atcgacctgg
    12421 cggaagaacg ttccggcaac atgcctaccc gcgaatggaa acagaaacgc tttaaaaaac
    12481 cgtggtatca gggtgacacc attccggttg gtatcggcca gggttactgg acagcgaccc
    12541 caatccagat gagtaaggca ctgatgatcc tgattaatga cggtatcgtg aaggttccgc
    12601 atttgctgat gagcaccgcc gaagacggca aacaggtgcc atgggtacaa ccgcatgaac
    12661 cgcccgtcgg cgatattcat tccggttact gggagctggc gaaagacggt atgtacggtg
    12721 ttgctaaccg ccctaatggt acggcgcata aatactttgc cagcgcaccg tacaaaattg
    12781 cggcgaaatc tggtaccgct caggtcttcg gtctgaaagc gaacgaaacc tataatgcgc
    12841 acaaaattgc cgagcgttta cgtgaccaca aactgatgac cgcctttgcg ccatacaaca
    12901 atccgcaagt ggctgtcgcc atgattctgg agaacggcgg cgcgggtccg gcggttggta
    12961 cgctgatgcg ccagatcctc gaccacatta tgctgggtga taacaacacc gatctgcctg
    13021 cggaaaatcc agcggttgcc gcagcggagg accattaatc atgacggata atccgaataa
    13081 aaaaacattc tgggataaag tccatctcga tcccacaatg ctgctgattt tactggcatt
    13141 gctggtttac agcgccctgg ttatctggag cgccagcggt caggatattg gcatgatgga
    13201 gcgtaaaatc ggccaaatcg cgatgggtct ggtcatcatg gtggtgatgg cgcaaattcc
    13261 tccacgcgtt tatgaaggct gggcccccta tctctatatc atctgtatta ttttgctggt
    13321 ggcggtagat gctttcggag ccatctctaa aggcgctcaa cgctggctgg acctcggtat
    13381 tgttcgtttt cagccgtcgg aaattgccaa aatagccgta ccactgatgg ttgcgcgctt
    13441 tatcaaccgc gacgtttgcc cgccatcgtt gaagaacact ggcatcgcgc tggtgctgat
    13501 atttatgccc acgctgctgg tggctgcaca gcctgacctg ggaacatcaa tcctcgttgc
    13561 gctttccggt ctgtttgtac tgttcctctc tggccttagc tggcgtctga ttggcgtcgc
    13621 agtagtgctg gtagcggcgt tcattccgat tctgtggttc ttcctgatgc atgattacca
    13681 gcgccagcgc gtaatgatgc tcctggaccc ggaatcagac ccactcggcg cgggctatca
    13741 cattattcag tctaaaattg ctattggctc cggcggatta cgcggcaaag gctggctgca
    13801 cggcactcag tcacagcttg aatttctccc cgaacgccat actgacttta tcttcgcggt
    13861 actggcggaa gagctgggat tagtgggcat tctgattctg ctcgctctct acattctgct
    13921 gatcatgcgc gggctgtgga tagccgccag agcgcaaacc acctttggtc gcgtcatggc
    13981 tggtggctta atgctgatat tattcgttta tgtcttcgta aatattggta tggtaagcgg
    14041 tattctgccg gttgtagggg ttccgctccc actggtcagt tatggaggat cggcgctaat
    14101 tgtgctgatg gctgggttcg ggattgtaat gtcaatccac acccacagga aaatgttgtc
    14161 gaaaagcgtg taagaggtgc gcaatgcgta agcagtggct cgggatctgc atcgcggcag
    14221 gaatgctcgc ggcatgtaca agcgatgatg gtcagcaaca gacggtaagt gtaccgcagc
    14281 ctgcggtatg taacggcccc atagttgaaa ttagcggggc ggacccgcgt ttcgaaccac
    14341 tgaacgcgac ggcaaatcag gattaccagc gcgacggtaa aagctacaaa atcgtgcagg
    14401 atccgtctcg atttagccag gcgggactgg cggcaatcta tgatgccgaa ccaggcagta
    14461 acctgacggc ctctggcgaa gctttcgatc cgacaaagct gacggcggcc catccaacgc
    14521 ttccgatccc cagctacgcc agaatcacta acctggctaa cgggcgaatg atcgtggtgc
    14581 gcattaatga tcgcggccct tacggcaacg accgcgttat ttcgctttct cgcgcggcag
    14641 ctgaccgtct taacacgtca aacaacacca aagttcgtat cgatccgatt attgtcgccc
    14701 aggatggttc gctttctggt cctggtatgg cttgtaccac agtcgccaaa cagacttatg
    14761 ccctgcctgc acctcccgat ttaagcggtg gcgcgggaac aagttcagtg tctggcccgc
    14821 agggtgacat tcttccggtc agtaattcga cgctaaaaag cgaagatccg accggcgcgc
    14881 cggtaaccag tagcggtttc ctcggcgctc aaacgacctt agcgcctggt gtactggaag
    14941 gcagcgaacc gacgcctgcg ccacagcccg ttgttacagc ttcgtcgaca acgcctgcaa
    15001 cctcgcctgc aatggtgaca ccgcaagccg cctcgcaaag cgccagcggc aactttatgg
    15061 tgcaagtcgg ggccgtaagc gatcaggctc gtgcgcaaca gtaccaacag caactgggac
    15121 agaagttcgg cgtccccggt cgcgtaactc aaaatggcgc ggtctggcgg atccagcttg
    15181 gcccattcgc cagcaaagcc gaagccagta ccttgcagca acgtttgcaa accgaagccc
    15241 aattacagtc atttattact accgcgcagt agcgtaaagc aggcatctga tgtgtcaatt
    15301 tctgtaaatg acgttaacaa agtcacgcgg aaagtcagat gcctgccggt agtgcatttg
    15361 ctatagtagg gcactttttt aattccatca cggatgtcgt agttcagacc atgaatacca
    15421 ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta
    15481 tctctgctgc acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga
    15541 tcgatgcgga gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga
    15601 acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg
    15661 gccaggcaat gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg
    15721 catgggccac cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca
    15781 tgcaggttcc ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt
    15841 gtgtcgccat ggccgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca
    15901 gctacgttaa cgcactgggc ctgaaaaata cccacttcca gacggtacat ggtctggatg
    15961 ctgatggtca gtacagctcc gcgcgagata tggcgctgat cggccaggca ttgatccgtg
    16021 acgtaccgaa tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc
    16081 agctgaaccg taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg
    16141 gacacactga caaagcaggt tacaaccttg ttgcttctgc gactgaaggc cagatgcgct
    16201 tgatttctgc ggtgatgggt ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac
    16261 tgctaacctg gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt
    16321 tcgcctcaga accggtttgg tttagtgatt ctgatcgcgc ttcgttaggg gttgataaag
    16381 acgtgtacct gaccattccg cgtggtcgca tgaaagatct gaaagccagc tatgtgctga
    16441 acagcagtga attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc
    16501 agcttgatgg caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa atcccggaag
    16561 gtaacttctt cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt
    16621 aaaaattgaa cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaaa
    16681 ctcccacctt ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac
    16741 caaacttaac gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc
    16801 gttacctgag ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta
    16861 caccccaacg gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa
    16921 cgccactcat atcgagcagg ttgaaacact gtatgaagaa ctgggcaaaa tcgatattgt
    16981 ccgcatggtt ctgtaactcg cttctccgtt acccggctcc tggtcgggta actctcctct
    17041 cctcgctgtg atatactttt cccccacttt tactcattct ccacggagat gccgttttgt
    17101 atcaggataa aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccggg
    17161 ctatgcatga attcaccgat acccgcgata atagtaccct tgatgaaatc tggctggtcg
    17221 agcactatcc ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg
    17281 gtgatattcc ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccgggac
    17341 aacaggtgat gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg
    17401 tgaccttgct tgagcaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc
    17461 ctcgggctga cgcgcctggt gtctatgtcg gggaaaagaa aatttgctca ctgggtttac
    17521 gaattcgacg cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac
    17581 catttttacg tattaatcct tgtgggtatg ccggaatgga aatggctaaa atatcacaat
    17641 ggaaacccga agcgacgact aataatattg ctccacgttt actggaaaat attttagcgc
    17701 tactaaacaa tccggacttc gaatatatta ccgcttaatt ccatacatca atggcccaat
    17761 ttacactggg tcattactca taatacctgc taatgcttta catctccttc gcgtttgtat
    17821 attttcttta tcacttgcag aaatgcggtg ccacgccttg ctccgtaact caccccattg
    17881 attaacctat tgaaatagtt gattatggtg agcaaaagcg ttccgttcca attagacata
    17941 agattattgt aattttttaa atattatttt tccatgaata aaattggagt aagtgcgtgg
    18001 atagtaataa tcaaattgaa ccctgtttaa gcagaaagtc gtcagaaggt aaaccacaaa
    18061 tatttacaac cttacgaaat attgatctta accttctgac tatttttgaa gctgtatatg
    18121 tacataaagg gatcgttaat gcagcgaaag tgcttaatct gaccccctcg gcaatcagtc
    18181 agtctattca gaaactgcgc gttatattcc ctgacccatt gtttattcgc aaaggccaga
    18241 gtgtcactcc taccgcattt gcgatgcatc tacatgagta tatcagtcag ggccttgagt
    18301 ccattcttgg cgcgctggat atcgaaggaa gctatgataa gcaacgaacg ataactattg
    18361 cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat ctatcgggcg attaaaactc
    18421 actatccgca gcttttactg cgcaacccgc ccatcagcga cgcggaaaac caactcagtc
    18481 agtttcaaac cgatctcatc atcgataaca tgttttgcac caatcgtacg gtgcaacatc
    18541 atgttctgtt caccgacaat atggtgttaa tttgccgcaa gggaaatcca ctactctctt
    18601 tagaagatga cagagagact atcgacaacg ctgcgcatgt acttctgtta ccggaagggc
    18661 aaaatttcag cggtctgcgg cagagagttc aagagatgtt tccggaccgg caaatcaatt
    18721 tcaccagcta caacattttg acaatcgctg cactggttgc caacagtgac atgttagcga
    18781 ttattcccag ccgtttttat aacctgttta gtcgttgccg gccgctggaa aaactgcctt
    18841 ttccgtcctt aaatgaggag caaatagatt tctctatcca ctacaacaaa ttcagcctgc
    18901 gtgatcccat cctgcacgga gtaatagatg ttatccgtaa tgcattttga aaaagtattg
    18961 ttacagccag atcacagtgc agaacgataa aatggcacaa tccaacaaca attttacatt
    19021 ttcgcgaccg ctttggctgc tttcaggtcc gtttcaatga tatactgcca gtcgttaatt
    19081 caaaaatagt tgataattac aacaatctat tgaattgaaa cgctttcctt cgtaattcgc
    19141 aactggaaca cgcacgctat gagtaaaccc attgtgatgg aacgcggtgt taaataccgc
    19201 gatgccgata agatggccct tatcccggtt aaaaacgtgg caacagagcg cgaagccctg
    19261 ctgcgcaagc cggaatggat gaaaatcaag cttccagcgg actctacacg tatccagggc
    19321 atcaaagctg caatgcgcaa aaatggcctg cattctgtct gcgaggaagc ctcctgccct
    19381 aacctggcgg aatgcttcaa ccacggcaca gcaacgttta tgatcctcgg cgctatttgt
    19441 acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc cggtagctcc tgatgccaat
    19501 gaaccagtga aactggcgca gaccattgcc gatatggcgc tgcgttatgt ggttatcacc
    19561 tccgttgacc gtgatgacct gcgcgatggc ggtgcccaac actttgcgga ttgtattact
    19621 gccattcggg aaaaaagccc gcaaatcaaa attgaaactc tggtgccgga tttccgcggt
    19681 cgaatggatc gtgctctgga tattctgact gcaacgcccc cagatgtgtt caaccataac
    19741 ctggaaaacg taccgcgtat ttaccggcag gtacggcctg gcgcagatta caactggtcg
    19801 ctgaagctgc tggaacgctt taaagaagcg catccggaaa tcccgaccaa gtctggtctg
    19861 atggtgggtc tgggtgaaac caatgaagaa attattgagg taatgcgcga cctgcgccgt
    19921 catggtgtga cgatgttaac gctggggcaa tatttgcagc caagccgcca tcacctgccg
    19981 gttcaacgtt acgttagccc ggatgagttc gacgaaatga aagccgaagc gctggcgatg
    20041 ggctttaccc atgctgcatg cggtccgttt gtccgctctt cttaccacgc cgatttgcag
    20101 gcgaaaggga tggaagttaa gtaatcctgt aataattact tacatctgtc tgataaaaaa
    20161 cccgcttttt gaagcgggtt ttttgtatcg aacaagatat tgagggagcg tcctgctcgc
    20221 cacgtcactc tttatgagag agcttttcag cctgaagatc aacgtctgcg ccttttttcg
    20281 ccgcagcatc gtcatcattc atcgccttct tgaacccttt aatggccgct ccaaggtctc
    20341 cgcccagcgt acgtaacttc ttagtcccaa acagcagaac gaccagcgcc gcaactacca
    20401 gcagtttggt aatactaatc tcacccatag ataccttctt gacatataac cggcaacaga
    20461 acgcgcctta aaaaccagag aatattaacg gtcattcgac gcgcgcacac aatagcaatc
    20521 tgtaacaagg tgaatcaagc gccgagtaaa aaaacatcat aataattgcg gcggcgcaaa
    20581 gcgacggttg tttaagatgg gcagttgcgc gcgcacctga cgcacacgtt cgggcgtcac
    20641 ttccgccata atgagtgcag gcatttctga cgctgccgca atggtgacac caaagggatc
    20701 tataatccgg ctttgcccga tatttttgtt cccgcactcc cccgccgcca ccatataaca
    20761 ggtggtatcc agcgcacgag cggcaagcaa cgttgaccag tgatgctctt tgagcggccc
    20821 acgaacccag gcggcaggaa gtaccaggat ttcagctccc tgtaaagcct gtgccagcgc
    20881 cagctctgga aagcgtaagt cataacaggt catcagaccg accttcatcc cttccacctc
    20941 cagtaacgga gctatttcat taccagcatc aacatggcgt gattcctgaa tggcaaatgc
    21001 atcatagaga tgcagtttgg cataacgggc gacgatgttt cctgcctgaa gtgccaccag
    21061 catattccat gcgcgccccg gcgttgaagg aagatgaatc gtcagaattg tcgtcatcat
    21121 gttacgttta ctttctcgcc gtaaacgtcc gaggaattcg ccttccagca gctgtgctga
    21181 tttaaccgat agatctgcat catgatcatc gcgcgccagc aatgcttccg gcaggacaaa
    21241 cagcgatacg tcgttttccg ccgcctgcgc catcaacgag gcacaaatct cagcgttctt
    21301 ttcccacaca gatgtaacag caaactgtcc tgcagcaacc agcattgcca cctcctgtca
    21361 gagtcgtaaa attcgccaac gacgattatt cggcgttaca cttatcactc atacaaatca
    21421 aatagcagga ttttgcagtg ttacaacttc ttttagcagt ttttattggc ggtggtacgg
    21481 gaagcgtagc gagatggctg ttaagtatgc gatttaaccc gctgcatcag gcgattccgt
    21541 tggggacgct ggcagcaaac ctgattgggg cattcatcat aggaatggga ttcgcctggt
    21601 tcagcaggat gacgaacatt gatccagtgt ggaaagtatt aatcaccacc ggattttgtg
    21661 gcggtctaac aaccttctca acattttcgg cagaagtggt gtttttgtta caagagggcc
    21721 gctttggctg ggcattactg aacgttttcg tcaaccttct ggggtctttt gccatgaccg
    21781 cactggcatt ctggctgttt tcggcctcaa ccgcacacta aagaccaaaa aaaacccgct
    21841 gattaagcgg gttttgaatt cttgctgacg tatcttacag agcgattacg tttgcagcag
    21901 aagggccttt ggcaccgtta gtgatttcga actctacgcg ctgaccttca gcaagagttt
    21961 taaaaccatt agtctggatt gcagagaagt gtacgaacac gtctttgctg ccgtcttccg
    22021 gagtaatgaa accgaatcct ttggactcat taaaccactt aacgttacct ttaatcttag
    22081 acatcaaaat tacctttaca tgaaaaatag acacaaatgc tgtgtcggtt atcagtacac
    22141 caattgtggt acgctttgtc cagtgcaact ttactaaaaa gtgataaaag tcgcgttatt
    22201 tttgatgttg cttattttag ttattgattt taaagaagtt actaaaactt catttgcctc
    22261 aaaactgaaa gcgcatccag gcaaagtaca cattgccatt gttgtaggta cccggaatgt
    22321 aggtcatctg aaaagtcact gggccataac ccacggaggc caatggcagt agaaccggga
    22381 gagggatgta attccagtta tcgcgtgccg ttacgccagc ggtgaatccc agacctaaat
    22441 gaaaattttc atccgccagc ggtcgccagg tactttccca tccgtatccg gcaatcggtt
    22501 cccatttgtt ccacgagtcc ttaaatgcca tggcatacag gccatgccag tttccttttt
    22561 catcccaacg cgacaggcca aaaccgccac cccacggtcg ctcgttatag cgatcggttt
    22621 tttctttgtc gtaagcgaaa cgtgcatgcc aggtgatggc aggaatatat aaatcataat
    22681 gttcaggctg ttgccaggtt tgtgcaatat tttctctaaa cgttgtcatc ctctcatctg
    22741 cgttagcaaa aactttacca acgctgatta actgaataaa aacaaaggaa aagatagcga
    22801 catatttact cacgttcatt tgtgaccata aaacatttat caaaaatcta ctactagcat
    22861 agcaaagcta caattaacat aaccttaata gacccaacat aaagaataat ctgaatagct
    22921 gctctattta tttccgcttg ttcaagttaa atatttccgc gcatcgatta aagatgatgc
    22981 gtcgttttct gcgatgggaa tagtcaaaaa agaaaaacca agtctttttt gatgacaaaa
    23041 tgcaatcaag gaaaaattat tttttttaac gagatacaac aatcatctta acgaagtata
    23101 taatatatat cgtcaaccca tgctcatatt agattatttt ttgagcgcac agccgcagct
    23161 aacaccattg caattaacaa atttgcatca atccaccatc aatttacaca cattattatg
    23221 tgataattgc caaccgctaa atatgcgttt tgttatctat gtataaaaac agcaacttca
    23281 atgtcttaat ggcagttttt cttgatttta atcagcattc atcgccaatt tattgggcat
    23341 attttttcct taagctttag gaagttttta ttatttactt tgggcctgga gacaggaaaa
    23401 attatgctga cattcattga actccttatt ggggttgtgg ttattgtggg tgtagctcgc
    23461 tacatcatta aagggtattc cgccactggt gtgttatttg tcggtggcct gttattgctg
    23521 attatcagtg ccattatggg gcacaaagtg ttaccgtcca gccaggcttc aacaggctac
    23581 agcgccacgg atatcgttga atacgttaaa atattactaa tgagccgcgg cggcgacctc
    23641 ggcatgatga ttatgatgct gtgtggcttt gccgcttaca tgacccatat cggcgcgaat
    23701 gatatggtgg tcaagctggc gtcaaaacca ttgcagtata ttaactcccc ttacctgctg
    23761 atgattgccg cctattttgt tgcttgtctg atgtcactgg ccgtctcttc cgcaaccggt
    23821 ctgggtgttt tgctgatggc aaccctgttt ccggtgatgg taaacgttgg tatcagtcgt
    23881 ggcgcagcag ctgccatttg tgcctccccg gcggcgatta ttctcgcacc gacttcaggg
    23941 gatgtggtgc tggcggcgca ggcttccgaa atgtcgctga ttgacttcgc cttcaaaacg
    24001 acgctgccta tctcaattgc tgcaattatc ggcatggcga tcgcccactt cttctggcaa
    24061 cgttatctgg ataaaaaaga gcacatctct catgaaatgt tagatgtcag tgaaatcacc
    24121 accactgccc ccgcgtttta tgccattttg ccgttcacgc cgatcatcgg tgtactgatt
    24181 tttgacggta aatggggtcc gcaattacac atcatcacta ttctggtgat ttgtatgctg
    24241 attgcctcca ttctggagtt cctccgcagc tttaataccc agaaagtttt ctctggtctg
    24301 gaagtggctt atcgcgggat ggcagatgcg tttgctaacg tggtgatgct gctggttgcc
    24361 gctggggtat tcgctcaggg gcttagcacc atcggcttta ttcaaagtct gatttctatc
    24421 gctacctcgt ttggttcggc gagtatcatc ctgatgctgg tattggtgat tctgaccatg
    24481 ctggcggcag tcacgaccgg ttcaggcaat gcgccgtttt atgcgtttgt tgagatgatc
    24541 ccgaaactgg cgcactcttc cggcattaac ccggcgtatt tgactatccc gatgctgcag
    24601 gcgtcaaacc tgggtcgtac cctatcaccc gtttctggcg tagtcgttgc ggttgccggg
    24661 atggcgaaaa tctcaccatt tgaagtcgta aaacgcacct cggtgccggt gcttgttggg
    24721 ctagtgattg ttatcgttgc tacagagctg atggtgccag gaacggcagc agcggtcaca
    24781 ggcaagtaaa tagtaatgcc ggaggcaagt ttcctccggc tttatcatgt cacccactgt
    24841 ggtatatgcg ttgtggtctg ccaactttgc cgtgaacaat ttcagcaata atcagatggc
    24901 ggctggcgca atattcaaga taacgcctgg cagtggtgcg gctgatggtc agtgcctgcg
    24961 ccaccgtttc tgccgtatgt tgcacaccag gctctttaaa cagttttcgc accgcgttta
    25021 gcgtcaaggg gtcaatgccg gtcggcagtt cgtctttggg ttcaccgcga gcataggcat
    25081 taaacatctc atcaatttgc ttctggctgg cgctatcaat actttccagc atatgtttac
    25141 gctggcggaa acgggttagc gtttgcccca gtgtattgat tcacttgaag tacgaaaaaa
    25201 ccgggagaac attgg
//
LOCUS       DAJTYG010000054        24417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTYG010000054 DAJTYG010000000
VERSION     DAJTYG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.054"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..489)
                     /locus_tag="OO568_002368"
     CDS             complement(<1..489)
                     /locus_tag="OO568_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6641699.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLE"
     gene            762..950
                     /locus_tag="OO568_002369"
     CDS             762..950
                     /locus_tag="OO568_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6641700.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            962..1672
                     /gene="bcsQ"
                     /locus_tag="OO568_002370"
     CDS             962..1672
                     /gene="bcsQ"
                     /locus_tag="OO568_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6641701.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAEEILTLA
                     NWCLLNYSGLKTPVGSAS"
     gene            1669..4246
                     /gene="bcsA"
                     /locus_tag="OO568_002371"
                     /pseudo
     CDS             1669..4246
                     /gene="bcsA"
                     /locus_tag="OO568_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            4257..6596
                     /gene="bcsB"
                     /locus_tag="OO568_002372"
     CDS             4257..6596
                     /gene="bcsB"
                     /locus_tag="OO568_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR6641702.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNLPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIREYGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6603..7709
                     /gene="bcsZ"
                     /locus_tag="OO568_002373"
     CDS             6603..7709
                     /gene="bcsZ"
                     /locus_tag="OO568_002373"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6641703.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7768..7944
                     /locus_tag="OO568_002374"
                     /pseudo
     CDS             7768..7944
                     /locus_tag="OO568_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            8272..10020
                     /gene="bcsC"
                     /locus_tag="OO568_002375"
                     /pseudo
     CDS             8272..10020
                     /gene="bcsC"
                     /locus_tag="OO568_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10102..12090
                     /gene="hmsP"
                     /locus_tag="OO568_002376"
     CDS             10102..12090
                     /gene="hmsP"
                     /locus_tag="OO568_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6641704.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12274..13560
                     /gene="dctA"
                     /locus_tag="OO568_002377"
     CDS             12274..13560
                     /gene="dctA"
                     /locus_tag="OO568_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6641705.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13781..15277
                     /locus_tag="OO568_002378"
     CDS             13781..15277
                     /locus_tag="OO568_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6641706.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15373..16302)
                     /gene="kdgK"
                     /locus_tag="OO568_002379"
     CDS             complement(15373..16302)
                     /gene="kdgK"
                     /locus_tag="OO568_002379"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6641707.1"
                     /translation="MSKKIAVIGECMIELSEKDADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGAGSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSVENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16534..17301
                     /gene="pdeH"
                     /locus_tag="OO568_002380"
     CDS             16534..17301
                     /gene="pdeH"
                     /locus_tag="OO568_002380"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6641708.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            17371..19422
                     /locus_tag="OO568_002381"
     CDS             17371..19422
                     /locus_tag="OO568_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6641709.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWSFRL
                     DNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFGLKA
                     QGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGGVDL
                     RLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGDSDI
                     HGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKSVQP
                     AGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQPLKF
                     GMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAELRG
                     SGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNCAAA
                     NLHIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSPLYV
                     RGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILSQMK
                     K"
     gene            complement(19604..20926)
                     /locus_tag="OO568_002382"
     CDS             complement(19604..20926)
                     /locus_tag="OO568_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6641710.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLAGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21337..22350)
                     /gene="yhjD"
                     /locus_tag="OO568_002383"
     CDS             complement(21337..22350)
                     /gene="yhjD"
                     /locus_tag="OO568_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6641711.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22399..23298)
                     /locus_tag="OO568_002384"
     CDS             complement(22399..23298)
                     /locus_tag="OO568_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6641712.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            23818..>24417
                     /locus_tag="OO568_002385"
     CDS             23818..>24417
                     /locus_tag="OO568_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6641713.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5786 a         6152 c         6711 g         5768 t
ORIGIN      
        1 ctcaagcaac aatgaaaatt gtttatcgtt attatttccg ggattaatta ccaaaagcga
       61 acaatggttt aacctgctcc atttattcat tttatccaac catgagcgaa gccgctcggc
      121 aggaatgttt tgccatgcgt tatttgcgca aacaagaata aaaaggtaat tatggggatc
      181 aatagaacac tgcaaatcac gggccaaata gtatagacct ttttcatgat ttagcattga
      241 aaataatttt attttttccg gcccttgaga atcatctaat tgaaagattt tcgccggatc
      301 gctgtccatg ctaatgaccg cgacgtgtgc ggtttcagcc tgggatgcaa ttgtttgatt
      361 cgccagactg atagcatctt catggcgatc gacgttaaac caccagacgc cgcctgctgg
      421 catatgtcgc agctcatccc ataatgatga gataccgata gagaatacag ggtccacaat
      481 gtccctcatg tcaccatttt gtctgattat cagtttacta gcgaaagcta agaaataaac
      541 ctaccattga aattaaaact tatcagattt agcatgtgaa tcaatttaga aggtcacaag
      601 acgtgtgatt ttagtctttt cgtttttaac tttttctgtt gcgtaacgga aagtcaaaaa
      661 gtgagcaaat tcccgtctcg ccgtaaacaa taaaccggcg aaatgctcac ggttagaatt
      721 gtatcatgaa tggtacggtt atttcatagg gatcaagcaa aatgaataac aatgaaccag
      781 atactctgcc tgatcccgcg ataggctata tcttccagaa tgatattgtg gcgttaaagc
      841 aggcattttc actgcctgat attgattatg ccgatatttc ccaacgcgaa cagttggccg
      901 cggcattaaa acgctggccg ttgctggcag agtttgcgca acaaaagtag gggattggtg
      961 aatggccgta ctgggattgc agggggtgcg gggaggcgtg gggacaacaa ccatcaccgc
     1021 cgcattagcc tggtcattac aaatgttggg agaaaatgtc ctagtggtcg atgcctgccc
     1081 ggacaacttg ttgcgcctgt catttaatgt tgattttacc caccgtcagg gctgggccag
     1141 agcgatgctg gatggccaag actggcgtga cgctgggttg cgctacacct cgcagctcga
     1201 tttgctgcct tttggtcagt tatccattga agaacaagaa aatccacagc actggcaaac
     1261 ccggctgagc gatatttgct ccggcttaca gcaactaaaa gccagcgggc gttaccagtg
     1321 gattttaatc gacttaccgc gtgatgcctc gcagataacc caccagctgc tgagtttgtg
     1381 cgagcactcg ctggcaatcg tcaatgtgga tgccaactgc catatccgac tgcatcagca
     1441 agcgctgccg gatggcgcac atattttgat taatgacttc cgtattggca gtcaggttca
     1501 ggacgatatt taccagcttt ggttgcaaag ccagcgccga ttactgccga tgctcattca
     1561 tcgtgatgaa gcgatggctg aatgcctggc ggctgaagag atactgacgc tggcgaactg
     1621 gtgcctgttg aactactccg ggctgaaaac gccagtcggg agtgcatcat gagtatcctg
     1681 acccggtggt tgcttatccc gccggtcaac gcgcggctta tcgggcgtta tcgcgattat
     1741 cgtcgtcacg gtgcgtcggc tttcagcgcg acgctcggct gtttctggat gatcctggcc
     1801 tggattttta ttccgctgga gcacccgcgc tggcagcgta ttcgcgcaga acataaaaac
     1861 ctgtatacgc caacgcctcg cgtccgcgtc cgctggaccc ggtccgttat ctcattcaaa
     1921 catgctggtt attgatcggt gcatcgcgca aagaaacgcc gaaaccgcgc agcgggcatt
     1981 ttcaggtctg caaaatattc gtggacgtta ccatcaatgg atgaacgagc tgcctgagcg
     2041 cgttagccat aaaacacagc atctggatga aaaaaaagag ctcggtcatt tgagtgccgg
     2101 ggcgcggcgg ttgatcctcg gtatcatcgt caccttctcg ctgattctgg cgttaatctg
     2161 cgttactcag ccgtttaacc cgctggcgca gtttatcttc ctgatgctgc tgtggggggt
     2221 agcgctgatg ttgattgtgc tgtcgctgac cgtttcttgc cgttatatct ggtggcgtta
     2281 cacctctacg ctgaactggg acgatccggt cagcctggtg tgcgggctta ttctgctctt
     2341 cgctgaaacg tacgcgtgga ttgtgctggt gctcggctac ttccaggtag tatggccgct
     2401 gaatcgtcag ccggtgccat tgccgaagga tatgtcgctg tggccgtcag tggatatctt
     2461 tgtcccgact tacaacgaag atctcaatgt ggtgaaaaat accatttacg cctcgctggg
     2521 tatcgactgg ccgaaagaca aactgaacat ctggatcctc gatgacggcg gcagggaaga
     2581 gtttcgccag tttgcgcaaa acgtgggggt gaaatatatc gcccgcacca ctcatgaaca
     2641 tgcgaaagcg ggcaacatca acaatgcgct gaaatatgcc aaaggcgagt tcgtgtcgat
     2701 tttcgactgc gaccacgtac caacgcgatc gttcctgcaa atgaccatgg gctggttcct
     2761 gaaagagaaa cagctggcga tgatgcagac tccacaccac ttcttctcgc cggacccgtt
     2821 tgaacgcaac ctgggacgtt ttcgtaaaac gccgaacgaa ggcacgctgt tctatggtct
     2881 ggtgcaggat ggcaacgata tgtgggacgc cactttcttc tgcggttcct gtgcggtgat
     2941 tcgtcgtaag ccgctggatg aaattggcgg cattgctgtc gaaaccgtga ctgaagatgc
     3001 gcatacttct ctgcgtttgc accgtcgtgg ctatacctcc gcgtatatgc gtattccgca
     3061 ggcggcgggg ctggcgaccg aaagtctgtc ggcgcatatc ggtcagcgta ttcgctgggc
     3121 gcgcgggatg gtacaaatct tccgtctcga taacccgctc accggtaaag gactgaagtt
     3181 tgctcagcgg ctgtgctacg tcaacgccat gttccacttc ttgtcgggca ttccacggct
     3241 gatcttcctg actgcgccgc tggcgttcct gctgcttcat gcctacatca tctatgcgcc
     3301 agcgttgatg atcgccctat tcgtgctgcc gcatatgatc catgccagcc tgaccaactc
     3361 gaagatccag ggcaaatatc gccactcttt ctggagtgaa atctacgaaa cggtgctggc
     3421 gtggtatatc gcaccaccga cgctggtggc gctgattaac ccgcacaaag gcaaatttaa
     3481 cgtcaccgcc aaaggtggac tggtggaaga agagtacgtc gactgggtga tctcgcggcc
     3541 ctacatcttc cttgttctac tcaacctggt gggcgttgcg gtaggcatct ggcgctactt
     3601 ctatggcccg ccaaccgaga tgctcaccgt ggtcgtcagt atggtgtggg tgttctacaa
     3661 cctgattgtt cttagcggcg cagttgcggt atcggtagaa agcaaacagg tacgccgatc
     3721 gcaccgcgtg gagatgacga tgcccgcggc aattgcccgc gaagatggtc acctcttctc
     3781 gtgtaccgtt caggatttct ccgacggtgg tttggggatc aagatcaacg gtcaggcgca
     3841 gattctggaa gggcagaaag tgaatctgtt gcttaaacgc ggtcagcagg aatacgtctt
     3901 cccgacccag gtggcgcgcg tgatgggtaa tgaagttggg ctgaaattaa tgccgctcac
     3961 cacccagcaa catatcgatt ttgtgcagtg tacgtttgcc cgtgcggata catgggcgct
     4021 ctggcaggac agctacccgg aagataagcc gctggaaagt ctgctggata ttctgaagct
     4081 cggcttccgt ggctaccgcc atctggcgga gtttgcgcct tcttcggtga agggcatatt
     4141 ccgtgtgctg acttctctgg tttcctgggt tgtatcgttt attccgcgcc gcccggagcg
     4201 gagcgaaacg gcacaaccat cggatcaggc tttggctcaa caatgatgat aacgcgatga
     4261 aaagaaaact attctggatt tgtgcagtgg ctatggggat gagtgcgttc ccctctttca
     4321 tgacgcaggc gacgccagca acgcaaccac tgatcaatgc tgagccagct gtagccgccc
     4381 agacggaaca aaatccgcag gtggggcaag tgatgtcagg cgagcaggga gctgatgccc
     4441 ccatcgtggc gcagaacggt ccttcgcgtg atgtgaagct gacctttgcg caaatcgcgc
     4501 cgccgccggg cagcatggtg ctacgtggta ttaacccgaa cggcagcatt gagtttggta
     4561 tgcgcagcga tgaagtggtg acgaaggcga tgctcaacct cgaatacacg ccatcgccat
     4621 cgttactgcc tgtccagtcg cagttaaagg tttatctcaa tgatgagctg atgggcgtgc
     4681 tgccagtgac caaagaacag ttgggtaaaa aaacgctggc gcaaatgccc attaatccac
     4741 tgtttattac cgacttcaac cgtgtgcggc tggagtttgt cggccattat caggacgtgt
     4801 gcgaaaaccc ggccagcacc acgctttggc tggatgttgg gcggagcagt ggactggatc
     4861 tgacctatca gaccctgaat gtgaagaatg acctgtcaca cttcccggtg ccattctttg
     4921 acccgcgcga taaccgtact aacaccttgc cgatggtctt tgcgggtgcg ccggatgttg
     4981 ggctgcaaca agcatctgcc attgtcgcct cgtggtttgg ttcgcgttct ggctggcgtg
     5041 ggcagaacct cccggtgctc tataaccaat tgccggatcg caatgccatt gtctttgcaa
     5101 ccaacgacaa acggccggac ttcctgcgcg atcatccggc ggtaaaagcc ccggtgattg
     5161 agatgattaa ccatccgcag aatccttacg tcaaactgct ggtggtgttt ggtcgtgacg
     5221 acaaagacct gttgcaggca gcgaaaggta tcgctcaggg taacattctg ttccgtggtg
     5281 aaagcgtggt agtgaatgaa gtgaaaccgc tgctaccgcg taagccgtac gatgcgccga
     5341 actgggtacg taccgatcgt ccggtcacat ttggcgaact gaaaacctat gaagaacagt
     5401 tacaatccag cggccttgag ccagcagcga ttaacgtttc gctaaacctg ccgccggatc
     5461 tctacctgat gcgcagtacc ggcattgata tggatattaa ttaccgctac accatgccgc
     5521 cggtgaaaga cagttcgcgg atggatatca gcctgaataa ccagttcctg caatccttca
     5581 acctgagcag caaacaggag gcgaaccgtc tgctgctgcg tattccggta ttacaaggtt
     5641 tgctggatgg caaaacagat gtctctattc cggcgctgaa actgggcgcg accaaccagc
     5701 tgcgcttcga ctttgagtat atgaacccga tgccaggcgg ttcggtagat aactgtatta
     5761 ccttccagcc ggtgcagaat catgtggtga ttggtgacga ttccaccatc gacttctcga
     5821 agtattacca cttcatcccg atgccggatc tacgcgcctt tgctaacgcg ggcttcccat
     5881 tcagccggat ggcggatctg tcgcaaacca tcaccgtgat gccgaaagcg cctaacgaag
     5941 cacagatgga aacgttgctg aatactgttg gttttatcgg cgcacagacg ggcttcccgg
     6001 cgattaatct gacggtgacc gatgatggca gcaccattca gggcaaagat gccgacatca
     6061 tgatcatcgg tggtatcccg gacaaactga aagacgataa gcagatcgac ctattggtgc
     6121 aggcgaccga aagctgggtg aaaacaccga tgcgccagac cccgttcccc ggcattgtgc
     6181 cggacgagag cgatcgcgcg gcagaaaccc agtcaacgct gacctcttcc ggtgcgatgg
     6241 cggcggtgat tggcttccag tcgccgtata acgaccagcg cagcgtgatt gcgctgttgg
     6301 cagatagccc acgcggttat gaaatgctta acgatgcggt gaacgatagc ggcaaacgcg
     6361 ccaccatgtt cggttcggtc gcggtgatcc gcgagtacgg tatcaacagc ctacgtgttg
     6421 gcgacgttta ttacgtaggt catctgccgt ggttcgagcg cttgtggtat gcgctggcaa
     6481 accatccgat tctgctggcg gtgctggcgg ctatcagtgt gatattgctg gcatgggtac
     6541 tgtggcgtct gctgcgaatt attagtcgtc gtcgtcttaa cccggataac gagtaattga
     6601 agatgaatgt gttgcgtagt ggaatcgtga cgatgctgtt gttggctgcc tttagtgttc
     6661 aggcagcctg tacctggcct gcctgggagc agtttaaaaa ggattacatc agtcaggaag
     6721 ggcgcgtcat cgaccccagc gacgcgcgca aaatcaccac ctccgaaggg caaagttacg
     6781 gtatgttctt tgccctggcg gctaacgacc gtgcagcttt cgataatatt ctcgactgga
     6841 cgcagaacaa tctcgctcag ggttctttaa aagaacgttt gcccgcctgg ctgtggggca
     6901 agaaagagaa cagtaagtgg gaagtgctgg acagcaattc ggcctccgat ggtgatgtct
     6961 ggatggcctg gtcgttgctg gaggcggggc gtttgtggaa agagcagcgt tataccgaca
     7021 tcggcagcgc gttgctaaaa cgtatcgcgc gggaggaagt ggtgacggtg cctgggctgg
     7081 gttccatgtt gttaccgggc aaagtgggtt ttgctgagga taacagctgg cgttttaacc
     7141 ccagctacat gccgccgacg ctggcgcagt atttcacccg ctttggcgcg ccgtggacca
     7201 cgctgcgcga aaccaatcaa catttattgc tggaaaccgc cccgaaaggc ttttcgccag
     7261 actgggtgcg ctatgagaaa gacaaaggct ggcagctaaa agccgaaaaa acattgatca
     7321 gcagctacga cgctatccgc gtttacatgt gggtaggcat gatgcctgac agcgatccgc
     7381 aaaaagcgcg gatgctcaac cggtttaaac cgatggcgac attcactgag aaaaacggtt
     7441 atccgccgga aaaagtggat gtggctacgg ggaaagcgca gggtaaagga ccggtcggtt
     7501 tttctgccgc catgctgcct tttttacaaa accgcgatgc gcaggcagtt cagcgccagc
     7561 gcgtggccga taactttccc ggcagcgata cctattacaa ctatgtgctg accctgtttg
     7621 gacaaggctg ggatcaacac cgtttccgct tctcgacaaa aggtgagtta ttacctgact
     7681 ggggccagga atgcgcaaat tcacactaaa catattcacg ctttccctcg gtctggccgt
     7741 catgccgatg gtcgaggcag caccaaccat cagcagaccc tgcggatgga cagcggcaac
     7801 actaatgccg tgcgcgggct ggcgaatatt taccgccagc agtcgccaga aaaagctgaa
     7861 gcgtttatcg cctcgctctc tgccagccag cggcgcagca ttgatgatat cgaacgcagc
     7921 ctgcaaaacg accgtctggc acagcaggca gaggtactgg aaaaccaggg caaatgggcg
     7981 caggcggcag cacttcagcg gcaacgactg gcgctggacc ccggcagcgt atggattact
     8041 taccgacttt cgcaggatct ctggcaggcc ggacaacgca gccaggccga tacgttaatg
     8101 cgcaatctgg cgcagcagaa gccgaacaac ccggagcagg tttacgctta cgggctgtac
     8161 ctctcaggtc acaaccagga cagagcggcg ctggcgcata tcaacagcct gccgcgcgcg
     8221 cagtggaaca gcaatattca ggagctggtt aatcgactgc aaagcgatca ggtgctggaa
     8281 accgctaatc gcctgcgaga aagcggcaaa gaggctgaag cggaagcgat gctgcgtcag
     8341 caaccacctt ccacgcgtat tgacctcacg ctggctgact gggcgtaaca acgacgtgat
     8401 tacaccgccg cccgcactgc atatcagaat gtcctgacgc gggagccaac taacgccgat
     8461 gccattcttg gtctgacgga agtggatatt gctgccggtg acaaagcggc ggcacgtagc
     8521 cagctggcga aactgcctgc caccgataac acctcgctga acactcagcg gcgcgtggcg
     8581 ctggcacagg cgcagcttgg agataccgca gcggcgcagc agacgtttaa taagttgatc
     8641 ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga tggtgctgcg cgacggtgcg
     8701 aagtttgaag cgcaggcggg cgatccaaag caggcgctgg aaacctacaa agacgccatg
     8761 gtcgcatccg gtgtgactac gacgcgtccg caggataacg acacctttac ccgactgacc
     8821 cgtaacgacg agaaagatga ctggctgaaa cgcggcgtgc gcagcgatgc ggcggacctc
     8881 tatcgccagc aggatcttaa cgtcaccctt gagcacgatt actggggttc gagcggcacc
     8941 ggtggttact ccgatctgaa agcgcacact accatgttgc aggtggatgc gccgtattct
     9001 gacgggcgga tgttctttcg cagtgatttc gtcaatatga acgtcggcag tttctccact
     9061 aatgccgatg gcaaatggga tgacaactgg ggcacctgta cattacagga ctgtagcggc
     9121 aaccgcagcc agtcggattc cggtgccagc gtggcggtcg gctggcgaaa tgacgtttgg
     9181 agctgggata tcggtaccac gccgatgggc ttcaacgtgg tggatgtggt cggcggcatc
     9241 agttacagcg atgatatcgg gccgctgggt tacaccgtta acgcccaccg tcggcccatc
     9301 tccagttctt tgctggcctt tggtgggcaa aaagactccc cgagcaatac cgggaaaaaa
     9361 tggggtggcg tacgtgccga cggtgtgggg ctaagtctga gctacgataa aggtgaagta
     9421 aacggcgtct gggcatcgct tagtggcgac cagttaaccg gtaaaaatgt cgaagataac
     9481 tggcgcgtgc gctggatgac gggctattac tataaggtca ttaaccagaa caatcgccgc
     9541 gtcacaatcg gcctgaacaa catgatctgg cattacgaca aagatctgag tggctactca
     9601 ctcggtcagg gcggttacta cagtccgcag gaatacctgt cgtttgccat accggtgatg
     9661 tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg cgtctggctc gtggtcgcat
     9721 tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc tgatcccgac cgactggcag
     9781 gaagaagctg cgcggcaatc caacgatggc ggcagcagtc agggcttcgg ctacacggcg
     9841 cgggcattac ttgaacgacg tgttacttcc aactggtttg ttggcacggc aattgatatc
     9901 cagcaggcga aagattacgc acccagccat ttcctgctct acgtacgtta ttccgccgcc
     9961 ggatggcagg gtgacatgga tttaccgccg cagccgctga taccttacgc cgactggtaa
    10021 gttttcagat agcgcctctc ttaatgccgc tgcgatcggg tatactcggg cggcaatctg
    10081 ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt taacaatcaa gcagatggca
    10141 atggtggcag ccgttgtcct ggtgttcgtt tttatttttt gcaccgtttt gctgttccat
    10201 ctggtccagc agaatcgcta taacacggct acgcaactgg aaagcattgc tcgctctgtc
    10261 cgcgaaccct tatcttcagc tattttgaaa ggcgatattc ccgaagcgga agctattctt
    10321 gccagcatta aaccggcagg cgtggtcagc cgtgccgatg tagtgctgcc taaccagttc
    10381 caggcgctgc gtaaaagttt tattccagag cgtccggtgc cggtaatggt tactcgcctg
    10441 tttgagctac cggttcaaat ctcgctgggc gtctactcgc tcgaacgtcc ggcaaatccg
    10501 cagccaatag cctatctggt gctacaggcg gattccttcc gtatgtataa gttcgtgatg
    10561 agcactctct caacgttagt gaccatttac ttacttttgt cgcttatcct gaccgtcgcc
    10621 atcagctggt gcattaaccg cctgattttg catccgttac gcaatattgc tcgcgaactt
    10681 aacgccatcc cagcccagga gcttgttggt caccaactgg cattaccgcg tctgcatcag
    10741 gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca accagcaatt gctgcagcgc
    10801 cattatgaag aacagaacga aaatgcgatg cgcttcccgg tgtcggattt gccgaacaaa
    10861 gccttgctga tggagatgct ggagcaggtt gtcgcgcgta aacaaaccac cgcgctgatg
    10921 atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc tgaaagaggc gcaacgagaa
    10981 attctgctgc tgacgctggt ggaaaaactc aaatcggtac tgtcgccacg tatgatcctc
    11041 gcgcagatta gcggttatga ctttgctgtc attgccaacg gtgtacagga accgtggcac
    11101 gcaatcactt tgggtcagca agtactcact atcatgagcg agcgcctgcc gattgaacgt
    11161 attcaactcc gtccgcactg tagcattggc gtggcgatgt tctacggcga tctcaccgcc
    11221 gaacagcttt acagtcgcgc tatttctgcg gcatttaccg ctcgccataa aggcaagaat
    11281 cagattcagt tctttgatcc gcagcagatg gaagcagccc agaagcggtt gacggaagag
    11341 agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa tttggttaca gccacaggtc
    11401 gagatgacca gcggtaaact ggtcagtgcg gaagtgttac tgcgtatcca gcaaccggat
    11461 ggcagttggg acctgccgga tggcttaatc gatcgcattg agtgctgtgg gctgatggtt
    11521 accgtcggtc actgggtgct ggaagagtcc tgtcgattgc ttgcagcctg gcaagagcgc
    11581 ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc aactgatgca cccgaatatg
    11641 gtggcggata tgctggaact gttaacccgc tatcgcattc agccgggaac actgattctg
    11701 gaagtgacag aaagccgacg tattgacgac cctcatgctg cggtggcaat cctccgtccg
    11761 ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg gtatgggcta cgcagggctg
    11821 cgtcagctgc agcatatgaa atcgttgcca atcgacgtac tgaaaatcga caaaatgttt
    11881 gttgaaggct tgccggaaga tagcagcatg attgctgcaa ttatcatgct ggcgcagagc
    11941 ctgaacttac aaatgattgc cgaaggcgtg gagactgaag cacaacgaga ctggctggca
    12001 aaagcgggcg ttggtattgc ccagggcttc ctttttgctc gcccactccc tattgaaatc
    12061 ttcgaagaga gttacctgga agaaaagtag ctaccccaaa ctgattacaa aactttaaaa
    12121 agtgctggtt tgtgcgagcc agctcaaact ttttaacctt tttgtttcaa ttatgatcca
    12181 ggtacatttc tgtgatgttg tctgggtgtt attttaaggc cgcaggtacc cccataacct
    12241 tacaagacct gtggttttac taaaggacac cctatgaaaa cctctctgtt taaaagcctt
    12301 tactttcagg tcctgacagc gatagccatt ggtattctcc ttggccattt ctatcctgaa
    12361 ataggcgagc aaatgaaacc gcttggcgac ggcttcgtta agctcattaa gatgatcatc
    12421 gctcctgtca tcttttgtac cgtcgtaacg ggcattgcgg gcatggaaag catgaaggcg
    12481 gtcggtcgta ccggcgcagt cgcactgctt tactttgaaa ttgtcagtac catcgcgctg
    12541 attattggtc ttatcatcgt taacgtcgtg cagcctggtg ccggaatgaa cgtcgatccg
    12601 gcaacgcttg atgcgaaagc ggtagcggtt tacgccgatc aggcgaaaga ccagggcatt
    12661 gtcgccttca ttatggatgt catcccggcg agcgtcattg gcgcatttgc cagcggtaac
    12721 attctgcagg tgctgctgtt tgccgtactg tttggttttg cgctccaccg tctgggcagc
    12781 aaaggccaac tgatttttaa cgtgatcgaa agtttctcgc aggtcatctt cggcatcatc
    12841 aatatgatca tgcgtctggc acctattggt gcgttcgggg caatggcgtt taccatcggt
    12901 aaatacggcg tcggcacact ggtgcaactg gggcagctga ttatctgttt ctacattacc
    12961 tgtatcctgt ttgtggtgct ggtattgggt tcaatcgcta aagcgactgg tttcagtatc
    13021 ttcaaattta tccgctacat ccgtgaagaa ctgctgattg tactggggac ttcatcttcc
    13081 gagtcggcgc tgccgcgtat gctcgacaag atggagaaac tcggctgccg taaatcggtg
    13141 gtggggctgg tcatcccgac aggctactcg tttaaccttg atggcacatc gatatacctg
    13201 acaatggcgg cggtgtttat cgcccaggcc actaacagtc agatggatat cgtccaccaa
    13261 atcacgctgt taatcgtgtt gctgctttct tctaaagggg cggcaggggt aacgggtagt
    13321 ggctttatcg tgctggcggc gacgctttct gcggtgggcc atttgccggt agcgggtctg
    13381 gcgctgatcc tcggtatcga ccgctttatg tcagaagctc gtgcgctaac taacctggtc
    13441 ggtaacggcg tagcgaccat tgtcgttgct aagtgggtga aagaactgga ccacaaaaaa
    13501 ctggacgatg tgctgaataa tcgtgcgccg gatggcaaaa cgcacgaatt atcctcttaa
    13561 tcccaccact tatgcccgca ctcccttccc cgagtgcggg tgtttgcgca taattgaccc
    13621 ctgtgcctga acttaccacc tgggtgagtt caggtttatt tcacttcttc cgtgacattt
    13681 tcatgttctt gcggtctaac acgaagtgtt tttaacgtca tttagactga ccgacaacgg
    13741 acagtttttt tattaccagg atagttgatc aggggttcac atgcagggca taaaaattcg
    13801 acttttagcg ggcggtttgc tgatgatggc cactgctggc tatgtgcagg cagatgcgct
    13861 ccagcctgat ccagcatggc aacaggggac gctttccaac ggtttacagt ggcaagtgct
    13921 gaccaccccc cagcgtccca gcgatcgtgt tgaaattcgc ctgctggtta ataccggttc
    13981 gctcgccgaa agtacacaac agagcggtta cagtcacgcc atccctcgta ttgcgctaac
    14041 gcaaagcggt ggccttgacg cagcacaggc gcgttcattg tggcagcagg ggatcgaccc
    14101 taaacgcccg atgccgccgg taattgtctc ttatgacacc acgctgttta atctgagttt
    14161 gcccaataac cgtaacgatt tgctgaaaga agcgctctct tatctggcaa atgccactgg
    14221 caaattgacc atcacaccag aaaccatcaa ccacgcgctg caaagtcagg acatggtggc
    14281 aacctggcct gccgatacta aagagggctg gtggcgctat cgtctgaaag ggtcaacctt
    14341 gttaggtcac gatcctgccg atccgctgaa acaacccgtt gaagcggaaa aaattaaaga
    14401 tttctatcag aaatggtaca ccccggatgc aatgacgcta ctggtggtgg gaaacgtgga
    14461 tgcgcgctcg gttgtcgacc aaatcaacaa aacgtttggc gaactgaaag gcaaacgtga
    14521 aacgccagct ccggtgccga cgctttctcc gctgcgtgcg gaagcggtga gtattatgac
    14581 tgacgcggtg cgtcaggacc ggttatctat catgtgggat acgccgtggc agccgattcg
    14641 tgaatcagcc gcactgctgc gctactggcg tgcggacctg gcccgcgagg cgctgttctg
    14701 gcatgttcag caagcgttaa gcgccagtga cagcaaagac atcggtcttg gatttgactg
    14761 ccgtgtgctg tatctgcgtg cgcagtgtgc catcaacatc gaatcaccaa acgacaagct
    14821 gaacagcaac cttaatctgg tggcgcgtga actggcgaag gttcgcgata aaggtctgcc
    14881 ggaagaagag ttcaatgcgt tagtggcgca aaagaaactg gagctgcaga aactgtttgc
    14941 cgcctatgca cgggctgata ccgatattct gatgggccag cggatgcgtt cgttgcaaaa
    15001 tcaggtcgtc gatatcgcgc cggagcagta tcagaaactg cggcaggatt tccttaacag
    15061 cctgactgtg gagatgttaa atcaggatct gcgtcagcag ttgtcgaatg atatggcgtt
    15121 aatactgctg cagccgaaag gcgagccgga atttaacatg aaagcgttgc aggcggcctg
    15181 ggatcaaatc atggccccat ctaccgccgc tgcgaccacc tctgtcgcca cggatgacgt
    15241 acatcctgaa gtgacggata ttccacctgc acagtaatgc ctgatggtga tgagttagca
    15301 caaccatcag gtatttatgg atgagctgga tagaggggta acactttatc cagccttttg
    15361 catatgctgc gtttacgctg gcatcgcctc acgcgggata atcgcgccgc gatactgaat
    15421 aacggtactt gcggtcaggt gcccgcgttt cgccgcgttt tccacactgc cgcccgtcaa
    15481 acgtaccgcc agataaccgg cgctgaaaga gtcgccagct gcggtggtat cgatcacttt
    15541 ttctttcggc agtttcaccg ccggaacatc aactaacgct tcaccagcaa tggacaccaa
    15601 gcaagaaccc gccccgcgtt tcaccaccac ttctttcacg cccgcgttat gggtgcgcgc
    15661 aatgacgtct tccaccggct gttgacccca cagcgcgtct tcgtcgtcca gcgtcaggaa
    15721 ggcgatatcc gtgcattcca gcatttgctg gtacacctgc tgtgtctctt ctttgctggc
    15781 ccacaggcgc ggacgatagt tattgtcgaa aatcactttt ccgcctttgg cgcggcattc
    15841 gcgcagcagg gaaagcagct tttcgcggct ggtcgggctt aagatcgcca ggctaatccc
    15901 gctcaggtag agataatcga aattcgccag ctcttcgcaa atcgccgcag actgctcgct
    15961 cgccagccag aatttggcgg cggcttcgtt acgccagtag tagaacgtac gttcgccggt
    16021 gctgtcggtt tcaatgtagt aaaggcccgg aagacggttt tccatccgtt gggtcaggga
    16081 agtatcaacg ttctcgccgt gccaggcgtc cagcatctgc tggctaaaac tgtccgttcc
    16141 cagcgccgtt acgtaatgaa cggttaatgc cgcaggatcg acctgacggg cgatatagac
    16201 ggaagtgttc agggtatcgc cgccgaaacc gcgcttaacg tccgcgtctt tctcggaaag
    16261 ctcaatcatg cattcgccaa tcacggcaat ctttttagac atagtcgtga acctgatctg
    16321 taaaaaatta gcgttagttt gcgctgtggt ggtttgctgg tcaaccatat taaaacagtg
    16381 ttccattatt tttttgagcc agaacgtatt cctgaaagat tccgttgtgg agtgaggaaa
    16441 tttaccccgg ccaggcggta aagttctgcc cttacgcgcc gataatcttt gtcgagtccg
    16501 ggcagcatca cttttaaaca caggacatct ttgatgataa ggcaggttat ccagcgaata
    16561 agcaaccctg aagcaagcat cgagagcttg caggaacggc gtttttggtt gcagtgtgag
    16621 cgtgcttaca cctggcagcc gatctatcaa acatgcgggc ggttaatggc cgtggagcta
    16681 ttaacggtgg tcacgcatcc cttgaaccct tcgcaacgcc tgccgccgga tcgctatttt
    16741 actgaaatca ccgtcagcca tcggatggag gttgtgaaag agcagattga tttgctggcg
    16801 caaaaagccg acttctttgt agagcacggc ctgctggcat cggtcaatat tgatggccct
    16861 acgctcatcg ccctgcgtca gcaaccaaaa atcctgcgcc agattgagcg tcttccctgg
    16921 ctgcgtttcg aactggtgga gcatatccgt ctgccgaaag attcaacctt tgcctcgatg
    16981 tgtgaatttg gcccgctgtg gctggatgat tttggtaccg ggatggcaaa tttctctgca
    17041 ctaagtgaag tgcgttatga ctacatcaaa atcgcgcgag aactgtttgt gatgctgcgt
    17101 cagtcgccgg aaggccgcac actcttttct cagcttttac atctaatgaa tcgctattgt
    17161 cgcggggtga ttgtcgaggg cgtagaaaca ccggaagagt ggcgtgatgt tcagaactcg
    17221 cccgcattcg ccgcacaagg ctggtttctt tcacgcccgg caccgatgga aacgctgaat
    17281 acggcggttc tggcgctata agctgcctca ttttccgccg ggctggacta tctttaggac
    17341 tggcacagga agaatatgag gaagcgaacg atgagcaagg caggcaaaat aaccgctgcg
    17401 atttcagggg ctttcttgtt gttgattgtc gtggcgatca ttttgattgc aacttttgac
    17461 tggaatcgac tcaaaccgac catcaaccag aaagtctctg cggagttgaa tcgtccgttc
    17521 gctatccgtg gcgatctggg cgtggtgtgg gagcggcaaa aacaagaaac tggctggcgc
    17581 agctgggtgc cgtggcctca tgtacatgca gaagacatca ttcttggtaa tccaccggat
    17641 attcccgaag tcacgatggt gcatttgccg cgagtagagg caccgctggc gctgctgacc
    17701 aaaacggtct ggctgccgtg gatcaagctc gaaaagcccg acgcgcgcct gattcgcctc
    17761 tctgaaaaga acaataactg gacgtttaat cttgccaacg atgacaacaa agacgcgaat
    17821 gcaaagccgt cggcatggtc gtttcggctg gataatattc ttttcgatca agggcggatc
    17881 gccattgatg acaaagtaag caaagcggat ctggaaattt ttgttgatcc gttaggcaag
    17941 ccgctgccat tcagcgaagt tactggatcg aaaggtaaag cggataaaga aaaggtgggc
    18001 gattacattt ttggcctgaa ggcgcaggga cgttataacg gtgaaccgct cacgggtacg
    18061 ggaaaaatag gcggtatgct ggcgctgcgt ggcgaaggga cgccgtttcc ggtacaggct
    18121 gatttccgtt caggtaatac ccgtgttgct tttgatggcg tcgtgaatga cccaatgaag
    18181 atgggcggtg tcgatttacg gcttaaattt tctggcgatt cactgggtga tctctatgaa
    18241 ctgacgggcg ttctgctgcc cgataccccg ccgtttgaaa cggatggtcg gctggtagcg
    18301 aaaatcgaca ctgaaaaatc gtcggtcttt gattatcgcg gttttaatgg gcgaattggc
    18361 gatagcgata tccacggttc tctggtctac accaccggaa agccacgacc aaaactggaa
    18421 ggtgatgtcg agtcgcggca attgcggctg gcggacctgg gaccgttgat tggcgttgat
    18481 tccgggaaag gggcagaaaa gtcgaaacgg tctgaacaga agaagggcga aaaaagcgtt
    18541 cagcctgcgg gcaaagtgct gccttatgac cgcttcgaaa ccgataaatg ggacgttatg
    18601 gatgccgatg ttcgcttcaa agggcggcgc attgagcatg gcagtagcct gccgattagc
    18661 gatctttcta ctcatatcat cctcaaaaat gctgacctgc gcctgcaacc gctgaaattt
    18721 ggcatggcgg gcggcagcat tgcggcgaat attcatctgg aaggcgataa aaagccgatg
    18781 caggggcggg cagatattca ggctcgtcga ctgaaactga aagaactgat gcccgatgtg
    18841 gaactgatgc agaagacgct gggggaaatg aacggtgacg cggaactacg cggtagcggt
    18901 aactcggtgg cggcgctttt aggcaacagt aacggcaacc tgaaactgtt gatgaatgac
    18961 gggctggtga gccgcaacct gatggagatt gttgggctga atgtcggcaa ctacattgtc
    19021 ggtgcgatat ttggtgacga tgaggtgcgg gtgaactgcg cggcggcgaa tctgcatatt
    19081 gccaacggcg tggcgcgccc gcagattttt gctttcgata ctgagaacgc gttgattaat
    19141 gttaccggca cggcaagttt tgcttcggaa cagctggatt tgactattga tccggagagt
    19201 aaagggattc ggattatcac actgcgttcg ccgctgtatg tgcgtgggac gtttaaaaat
    19261 ccgcaggctg gggtgaaagc cggaccgctg attgcccgtg gtgctgttgc tgcggcactg
    19321 gcaacgctgg taacaccggc ggcggcgtta ctggcactga tctcaccttc cgaaggggag
    19381 gctaatcagt gtcggacgat attgtcgcag atgaagaagt gatgataatt gccggatgtc
    19441 tgacatccgg caattaaaca gatttagatt tttggcaaag tgtgctttgt tcatgccgga
    19501 tgcggcgtga acgccttatc cggcctacaa atccttgtga attcaatata ttgtatggaa
    19561 attcagtcct gataagcgta gcgcatcagg cttttcacac ttattacaac gactgatgtc
    19621 gcgtctcatg ggtcagcagc agggcgatta acgtcaagcc agccatcgcc gccagatata
    19681 accccaccgc acctaacccg tagttagcct gcaaccaagc tgcgatatac ggcgcaacgg
    19741 aagccccgag aatcgacgat acgttgtaag agaacgatgc tccggtgtaa cgcacttctg
    19801 tcggaaacag ctctggtaac agcgcaccca tcggcccgaa ggtcagcccc atcagactta
    19861 accccagcag caggaaggca aaaaccagaa tcgggttgcc agaaccgagc agcgggttaa
    19921 aggcgaacag cgcgaacagg atgatcagcg tggtgatgat caccatgctt ttacggcgac
    19981 caaaggcatc agccagtaat ccggcgactg gcaccatcac gccaaaacca ataactgcca
    20041 tcatcagcat ccacaacact tcgttacgcg gcaggccaag cccaactggc gcggcggcgg
    20101 tactgaaggt catagagtag accgtcatga tgtaaaacag cgtataggtt gccagcataa
    20161 tgaacgtacc cagtacggtt acgcgaacat gtttggtcag cagcgtaccc agcgggatct
    20221 tcacctgttt tttcgcttta gcgactttct caaacaccgg cgactcatgc agcgacacgc
    20281 gaacatacag gccgataatg accagcaccg ccgagaagat aaatggcaca cgccagcccc
    20341 agctcataaa ctgctcatcg gtcagtagcc aggaaagcag caagaaagtg ccgttggcaa
    20401 agaagaagcc aatcggtgcg cctagctgtg ggaaggagcc atacagtgca cgtttgcgcg
    20461 gtggggcgtt ttcagtcgcc agcagcgccg cgccgcccca ttcaccgcct aagcccagac
    20521 cctgaccaaa tcgagccagc gccagcagca gtggggcgaa aataccaatc gtggcatagc
    20581 ccgccagcag accaatcacc acggtcgaaa tccccatcgt tagcaacgag gcgaccagcg
    20641 tcgctttacg cccaacgcga tcgccaaaat gaccaaaaac ggcagagcca atggggcgcg
    20701 cgacgaaggc gatggcgaag gtggcgagcg actgtagcgt tgctgccgtt ggatcgccct
    20761 gcgggaagaa gatatgcgga aacacaataa cggccgcagt ggcgtaaatg taaaagtcga
    20821 agaactcaat ggccgtgcca atgagagagg cgacaaggac tttattacgc gagttgatcg
    20881 gcgtgtattc ttgttcgtgg tcgagtgttg tggctgttgc ttgcataatc ttttcttatt
    20941 ttttggctaa cgaatagcca tattacgcac agcaaaagtg atatttcaat gtgtaacaaa
    21001 tcgcggaaat ggtcaaaaac caggcaaaaa ggggataatg tttaggccag tgatttttct
    21061 tctatgaaat gcttcacata attttataaa tagtggataa aactggtttc aggttaaata
    21121 aaagttacca actggattta tatgctgggg ttatgagttg gagtgaaatt gtttgctccc
    21181 aaatagatgg ctgaagaggc tctggggaag agtttcaaga ggttatgtgc gcattatagc
    21241 cggatgcgat gctggcgcat cttatccggc ctacggggag ccaggccgaa taaaatcgta
    21301 tgccggataa ggcgtttacg ccgcatccgg cctattttaa ggctgcgttt tccctggcat
    21361 tcgcgggtcg tctttatatt cggcggtggc aatccacgcc gcgcaaaaca gcgtcaaacg
    21421 ggcgaagaag tagaaaaatg ccatcagccc cagcacggaa ccaaatgctg cgccagaggg
    21481 ggatttcatc agcgatggca gggtgtaggt catcacgatt ttaatcactt caaaaccaat
    21541 agcagccaga aatgttccgc gaatcagggc ttttttgcgc ggacggtggc gcggcaaccg
    21601 ccagaagatc cagaagaaaa gcagatagtt ggcgaagatg gaaatcgcca ggccaatcaa
    21661 tcgccacgtc ggtttcagcc actcaatgct gttcaggtgc agggcgctaa taatcatttg
    21721 ctgcgccgaa ccggcaaccg aggtgatcga aagcgtcaca atcagcgcaa tcaacaaacc
    21781 aatcagtgaa ataaaatcac gcagatattt tacccagaac ttctcctgat cttgcggcga
    21841 gcgttcccag acatcgcgcg actgggcacg aatcgcttca cgcaggttac ccatccagtt
    21901 gatgccggaa taaagtgcca ccgccaggcc gacaagccct acagtcgtac gctgctgaac
    21961 ggcggtgttg atggtgtttt tcaacgtggc ggctagtgtc ggatcgctga tgttttgcag
    22021 aattttgtcg aagatatcct gtagcaacat cggatgggag gccagcacaa agccccctgc
    22081 ggcaaacgac accatcaaaa tcgggatcat cgacaaaaat gagaaatagg tgatagccgc
    22141 accaaactgg ttacccagcc gatcgttaaa gcgttctgtc gcgcgaataa ggtgcgcaat
    22201 aaccggttgg cgttggactt tttcggcagt ggtggtgacg gtttccagcg cctggctaac
    22261 tttgccgcct ttctcgctat tatccaactg cttaatcggc tcgtgctcca gatcctgggt
    22321 gggacgtttg atctcgtttt cctgcgtcat ccgctctttt gtccttctgt cgtggtcatt
    22381 cttaaaaagt atagtcggtc agtccaggtg ctctttcatt acgccagcca gccactgcat
    22441 aaacaggttt acacgccggg aaagctcccg acgctgcggg taaaccagcg aaagggagag
    22501 cggctcggca cggtagcctg gcaacacttc aataagccgc ccggcacgca gggcttcgcg
    22561 cacggcaatg cgcgggatct gaataatccc cagaccggca agaccggcgg cgagatagtt
    22621 ttcgctactg tttaccgtca acatgccgcc agacttaaac cactggacgc cattaacgct
    22681 ggcaacctca aaacctaacg gatgtacacc caggtgcggt gtataacgca ctattgcgtg
    22741 tgaagtcaga tcatcggggc tttgcggata accaaagcgc gtcagatagt gcggactggc
    22801 acagttgacc atggttagtt tgccgagggg acgggcgata acgccatctt ccggtaacgc
    22861 gccagtgcgt atcacgcaat caaaaccatc atgaagaata tctaccggac ggttatggct
    22921 actcagttcc agctcaattc ccggatgctg atagagaaat tccgacagac gcggcagtaa
    22981 caggtttttc gcgattcctg acgggatgtc gatgcgtaat ttaccgctga tactggtggc
    23041 atcctgttga aacagaccgt ccagttcgct gagattactc aacacatctt ttgcccgttg
    23101 ataataggtc atgccttctg gcgtgagttt gacccgtcgc gtagtgcgct ggagaagctg
    23161 ggtgccaagt tgatgttcca gtgcctgtat ctggcgcgaa acacttccct ttggcaaagc
    23221 aaagaaatcc gccgcgcggg aaaaactttc cagctccgcg actttgatga acaactgcat
    23281 tgcgtgaatt ttatccattt ctttgaccga ttgttgttca cagcgaaaca gtaacgcgca
    23341 tatcgcattg tttattgttt tttctaacat ctaatgccgg gtttcacaat gttgtcatga
    23401 cgaatgtggc gatttctcag caggaaaagg gcggaatagc tttatgagaa aggtgaatcc
    23461 ccttaaacag agcgtatttt tcagtgattc cctttaaagt gaatagagga cgtattggct
    23521 atttgtgctt atttttgtct cattgccatt tgttattttg gcttgtagtc gcggaatttc
    23581 atttttaatt gcatgcgtac taaccaccaa tataaggaaa tcctcaaaat tgttttaaat
    23641 agtgccctcg acatgtaatt tcaggcgttt agctgtaact atttatatcg gtaactattt
    23701 tctacgcgtc atctctgtag aatggccacc gccaaatggc gaattcttat tttttctcgc
    23761 gggtaatacc cttccactac ttaagtcttt ttaacgtatt caggtggaga ttcagttatg
    23821 caaatagtca tgtttgacag gcagtcaata tttattcatg gaatgaaaat cagtttacag
    23881 cagcgtattc caggagtgag tattcagggg gtcagtcagg cagacgagtt atggcaaaag
    23941 ctggaaagtt accctgaagc cttagttatg ctcgatggcg atcaggatgg tgagttttgc
    24001 tactggttgc tgcaaaaaac cgtggtgcag tttcctgagg ttaaggtgtt aatcacggcg
    24061 acggattgca ataaacggtg gttacaggaa gttatccatt ttaatgtgct ggccattgtg
    24121 cctcgcgatt caaccgtcga gacatttgcg ttggcggtaa atagtgcggc gatggggatg
    24181 atgtttctgc ctggagactg gcggacgacg ccggaaaagg acattaaaga tctcaaatcg
    24241 ttaagcgccc gccagcggga gattttaacc atgttagcag cgggcgaatc aaataaggag
    24301 atcggcagag cgctgaatat cagtaccgga acggtaaaag cgcatctgga atccctgtat
    24361 cgccgtctgg aagtgaaaaa tcgcacccag gcggcaatga tgttaaatat ctcctcc
//
LOCUS       DAJTYG010000055        23985 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTYG010000055 DAJTYG010000000
VERSION     DAJTYG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.055"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(1..573)
                     /locus_tag="OO568_002386"
     CDS             complement(1..573)
                     /locus_tag="OO568_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6641714.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            791..1651
                     /gene="tesB"
                     /locus_tag="OO568_002387"
                     /pseudo
     CDS             791..1651
                     /gene="tesB"
                     /locus_tag="OO568_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
     gene            complement(1700..2986)
                     /gene="amtB"
                     /locus_tag="OO568_002388"
     CDS             complement(1700..2986)
                     /gene="amtB"
                     /locus_tag="OO568_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6641715.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKLVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3016..3354)
                     /gene="glnK"
                     /locus_tag="OO568_002389"
     CDS             complement(3016..3354)
                     /gene="glnK"
                     /locus_tag="OO568_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6641716.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3535..5316)
                     /locus_tag="OO568_002390"
     CDS             complement(3535..5316)
                     /locus_tag="OO568_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6641717.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYNTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5309..7081)
                     /locus_tag="OO568_002391"
     CDS             complement(5309..7081)
                     /locus_tag="OO568_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6641718.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSELVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIHEEAVDA"
     gene            complement(7111..7569)
                     /gene="decR"
                     /locus_tag="OO568_002392"
                     /pseudo
     CDS             complement(7111..7569)
                     /gene="decR"
                     /locus_tag="OO568_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            complement(7723..8541)
                     /gene="cof"
                     /locus_tag="OO568_002393"
     CDS             complement(7723..8541)
                     /gene="cof"
                     /locus_tag="OO568_002393"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6641719.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLSSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8641..10340
                     /locus_tag="OO568_002394"
                     /pseudo
     CDS             8641..10340
                     /locus_tag="OO568_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10405..11100
                     /gene="queC"
                     /locus_tag="OO568_002395"
     CDS             10405..11100
                     /gene="queC"
                     /locus_tag="OO568_002395"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6641720.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11152..11550)
                     /gene="fadM"
                     /locus_tag="OO568_002396"
                     /pseudo
     CDS             complement(11152..11550)
                     /gene="fadM"
                     /locus_tag="OO568_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
     gene            complement(11644..12015)
                     /locus_tag="OO568_002397"
     CDS             complement(11644..12015)
                     /locus_tag="OO568_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6641721.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12166..14037)
                     /gene="ppiD"
                     /locus_tag="OO568_002398"
     CDS             complement(12166..14037)
                     /gene="ppiD"
                     /locus_tag="OO568_002398"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6641722.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNETESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14229..14501)
                     /gene="hupB"
                     /locus_tag="OO568_002399"
     CDS             complement(14229..14501)
                     /gene="hupB"
                     /locus_tag="OO568_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6641723.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14710..17064)
                     /gene="lon"
                     /locus_tag="OO568_002400"
     CDS             complement(14710..17064)
                     /gene="lon"
                     /locus_tag="OO568_002400"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6641724.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17252..18526)
                     /gene="clpX"
                     /locus_tag="OO568_002401"
     CDS             complement(17252..18526)
                     /gene="clpX"
                     /locus_tag="OO568_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6641725.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELDKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18652..19275)
                     /gene="clpP"
                     /locus_tag="OO568_002402"
     CDS             complement(18652..19275)
                     /gene="clpP"
                     /locus_tag="OO568_002402"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6641726.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19522..20820)
                     /gene="tig"
                     /locus_tag="OO568_002403"
     CDS             complement(19522..20820)
                     /gene="tig"
                     /locus_tag="OO568_002403"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6641727.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21164..21481)
                     /gene="bolA"
                     /locus_tag="OO568_002404"
     CDS             complement(21164..21481)
                     /gene="bolA"
                     /locus_tag="OO568_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6641728.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21786..22364
                     /locus_tag="OO568_002405"
     CDS             21786..22364
                     /locus_tag="OO568_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6641729.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22408..23883
                     /gene="ampG"
                     /locus_tag="OO568_002406"
     CDS             22408..23883
                     /gene="ampG"
                     /locus_tag="OO568_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6641730.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5624 a         6613 c         5799 g         5949 t
ORIGIN      
        1 ttaatattgc gtaggagcgg gaactgccga agacggattc acctgagttg gtgaagtcga
       61 aggtacggta gtcgttgcgc caccgctggc ctgaaccggc acggcggttt gctgtaccgg
      121 caccaatgtc aggtcggctt tagttccgcc ctggttgatc accggctgaa cggtatcggt
      181 gataaatacc agtttgtcat tcacggtaat cgccgcactc aacagaatac gcgcgttcgg
      241 ctgaacatct gccgggttaa atggcagaac aaagctgaat ggtgactgtt taccttcagt
      301 acgcaccgct ttctgcgcca gcacttttga cggtgcatcg gctaacgacg cgtcagaaag
      361 tgtcacggtc agcacagcat caggcggcag tgcgactttc tgacggatcc agacggtacc
      421 ggagacattc ggttgctgga tagcaggttg ttgtgttgct gaaatagacg tatttgcagc
      481 cggggctggc gtctgaatat ccgcgctttt atctgcgcaa gccgccaacg caatcgcaac
      541 cgctaaacca ctggccatgt gcacgagttt cattcatttc tccttattat caatgcacca
      601 gcgggctaac tttcctcgcc ggaagagtgg ttaacaaaat agtaacgtca acaagtgtgg
      661 cacacatcac gcatttctgc ctgtaattag cccgtaattc agactattgc acccatcgga
      721 ccagtggcaa atttgcgtta tactcaactc actttggctt gctgcggcag ctttgttact
      781 ggagagttat atgagtcagg cgctaaaaaa tttactgaca ttgttaaatc tggaaaaaat
      841 tgaggaagga ctctttcgcg gccagagtga agatttaggt ttacgctagg tgtttggcgg
      901 ccaggtcgtg ggtcaggcct tgtatgctgc aaaagagacc gtcccggaag agcggctggt
      961 acattcgttt cacagctact ttcttcgccc aggcgatagt aagaaaccga ttatttatga
     1021 tgtcgaaacg ctgcgtgacg gtaacagctt cagcgcccgc cgggttgctg ctattcaaaa
     1081 cggcaaaccg attttttata tgacggcctc tttccaggca ccagaagcgg gtttcgaaca
     1141 tcaaaaaaca atgccgtccg cgccagcgcc tgatggcctg ccttcggaaa cgcaaatcgc
     1201 ccaatcgctg gcgcacctgc tgccgccagt gcttaaagat aaattcatct gcgatcgtcc
     1261 gctggaagtc cgtccggtga agtttcataa cccactgaaa ggtcacgtcg cagaaccaca
     1321 tcgtcaggtg tggatccgcg caaatggtag cgtgccagat gacctgcgcg ttcatcagta
     1381 tctgctcggt tacgcttctg atcttaactt cctgccggta gctctacagc cgcactgcat
     1441 cggttttctc gaaccgggga ttcagattgc caccattgac cattccatgt ggttccatcg
     1501 cccgtttaat ttgaatgaat ggctgctgta tagcgtggag agcacctcgg cgtccagcgc
     1561 acgcggcttt gtgcgcggtg agttttatac ccaagacggc gtgctggttg cctcgaccgt
     1621 tcaggaaggg gtaatgcgta atcacaatta aaaaaatagc cggaggtgaa aaccgtccgg
     1681 ctattttttg cagtgcttgt tacgcgttat aggcattctc gccgtggctg ttgacgtcca
     1741 gcccttctcg ctcttgttct tccggcacac gtaggccaac cgtcagatcc gccagtttgt
     1801 agccaataaa tgccaccaca ccggaccaga cgatcgtaat ggcgatgctt tccaactgta
     1861 ccagcaactg atggcccatc gtcacacctt cagcgaagcc cacgccgccc agagaactgg
     1921 cggcaaaaat cccggtcatg atacagccga caatgccaca aacgccgtgc acaccgaaga
     1981 catcgcaggg atcatccacc cgcagcaagc gtttgagcat ggtaacgccc cacaaacccg
     2041 ccagaccagc taccacgccg ataatcaacg cgccgccaac cccaatgtag ccgcacgctg
     2101 gcgtcactcc gaccagaccg gcaatcgcgc cagaacacgc tcccagcaga gaaggcttac
     2161 cacgcagcgc ccattcaccg aagatccagc caagaattgc cgccgccgtt gcgaccacag
     2221 tattcacaaa tgccagtgcc gcgatttcat tcgccgtgcc cgctgacccg gcgttaaagc
     2281 caaaccaacc gatatagaga atggcagtcc cggtgaagac catcggcagg ttgtgcggtt
     2341 taaacgcctc tttaccgaag cccacgagtt tgcctatcag atacgcgccc accagaccgg
     2401 cgattgctgc gttaatgtgc accaccgtgc cacccgcgaa atccagcgca ccgtgagaag
     2461 ccagcaaacc accgccccac accatatgcg caatcggaat gtaagagagc gtcagccata
     2521 ccaccacgaa aatcaacaca gctgagaagc ggattcgttc cgccagcgcc ccaactatca
     2581 agcctacggt aatgcaggca aacgatccct gaaacgctac gtggatatac tgataaatgc
     2641 tgcccatcac cgccgtcagt tcgatgtttt ttagcatcaa ccagttaatg ttgccgaaga
     2701 agttgttgcc ctcaccaaac gccagcgaat aaccgtaaac cacccagaga atacagacca
     2761 gtgcaaacgt caccgtcacc tgcgtcagca tcgacagcac gtttttgccg cgaatcaacc
     2821 caccgtaaaa cagggcaatc cccggaatag tcataaacag caccagcgca gtacaaatca
     2881 tcataaacgc attgtcggct ttatcggcca ccgcaggtgc agccattacc agtcccggca
     2941 gcatcgccag tgaagcaagc ccagttttta tcgtcgctat cttcattttt cgttcctgtt
     3001 gctgtgtgcc agagattaca gcgccgcttc gtcggcttcg ccggtacgaa tacgaatgac
     3061 gcgttgcaat tcagcgacga agattttgcc gtcgccaatt tttccggtgt aagccgcctt
     3121 actgacgata tcgatcactt catcgagttg gtcatcagca atcgccacat caatttttac
     3181 ttttggcagg aagttgacgc tgtattccgc cccccggtac agctcggcat gccctttctg
     3241 acgcccgaaa cctttcactt cggtgacggt caggccctga ataccaatgg aagataacgc
     3301 ttcacgaacg tcttccagct tgaatggttt gattatcacg gtcaccagct tcatagatcc
     3361 cctccggtca gaattcggta atggttctgc tacacgaaga aggtattgca agcggtgtgc
     3421 cagaaatgaa aaacgagaaa gagagcagga agttaagaag attgaaaatg aaaacgcact
     3481 atgacagtgc acagtgcgtt acatttttgc accaacgatg aaatattgcg ctattcaggc
     3541 gctcaatgat tcctcttcac gcacgctggc tgccagctct tcgcccgcaa gttgcagttg
     3601 atacatctgc cagtagcgtc cctgggccgc cagcagttgc tggtgagtgc cctgctccac
     3661 ggcttgccca cgatgaagca ccagaatggt gtcggcatca ataatagttg ataagcggtg
     3721 agcaatcacc accagcgtgg tatgttcacg caccgccgcc agagcatgtt gaatcgcctg
     3781 ttcagtaccg gagtcaatgc tggcggttgc ctcatcaagg atcaggattt gcggcgtctc
     3841 caccagcacg cgcgccagtg ccagcagttg cttttgcccg actgagagat tatttccctg
     3901 ctcgcccagc ggcgtataaa tacaatcact catgctgcgc gccagctcag ccagttgcac
     3961 ggtttccagc gcctgccaga cgcgttcttc ggagatatcc cgccccagcg tcacgttggc
     4021 gaggaaggta tccgccagca ccaccggatc ttgctgcacc attgccacgc cctggcgcag
     4081 cgcgctatga cttagcgaac ttaatggacg accatcaagg tgaatctcac cttccgttag
     4141 cgggtaatag cccatcaata aactggcaag ggtactttta ccactgccgg tatgcccgac
     4201 cagcgccaca aagttacgcg aaggcacaga gagattaatg ttctttagca ccagattgtc
     4261 atcgcgataa gcaaatgaca cgttatcgac ttcgatggtg ccactctgta acgggtaatc
     4321 atcattgcca tattgctggc gcggtccgtc catcagttca aacacgcgct caccagcaac
     4381 aacagcctgt tgcagcatcg cctgttgcgt ggtcagttcg attaagggtt cgttaagtcg
     4441 cccaagatag ctgataaacg catacaacac gcccacttca atggtgccgc tggcggagaa
     4501 gccaaacagc atcaataagc cacaaagaat gagcgatgaa aacagactca gcagcggacg
     4561 cagcagaaaa ccgtcgaggc gcagggtttg catcctagcc atatagtgtg aacgactggc
     4621 ctcccccata cgttcgccaa atcgcgcctg ctgacgaaac tgctggataa cgctcatgcc
     4681 attgatgatt tcgttaaagc cgtcgttgat atccgccaga taggcgcgca cacggcggac
     4741 aatcggcgtg ttgtaacgct ggtatatcac cattaccacc agcaccaccg ggaaaatcat
     4801 tatcgccacc agcgccattc gccagtcgag gctgaacatc gccaccagca tcgcgcccac
     4861 cagtgcggca ctgcgcagga cagttgccac tacggtaacg tagagatcgc ggatcacttc
     4921 agtgtcatta gtgacgcggg agatcacctg cccgacgggt tgggtatcaa actcgcttaa
     4981 tggctggcgt aacgcagcat ccatcacgtc ggtacgcaac tgttgcacta cgcctactgc
     5041 cgcccgatta aacagcagcg actgagcgta atgtagcccg gcggcaaaca gttgtagccc
     5101 aacatacgcc gcagccagcc ctgcaaccac tttcaacggc aggttatttt tcgctaccat
     5161 attgtcgata aaatagctga taagcagcgg cccactgact tctgccgccg ccgcaaccca
     5221 catcatcagg accgcaatcc ccagcggttt acgccacggc gaaccatacg ctaacaggcg
     5281 cttgagagtc ggccacagtt ggctaaaact acgcatcgac ggcctcctcg tgaatttccg
     5341 gaacgtcgtc gagcgccgcc tccagttgtt gatagcgata catatcgcga taccagccgc
     5401 tttgttgcgc cagctcatca tgattgccac gctgggcgat atgtccgtgc tgcatcacaa
     5461 taatttcact ggcttccgtc agtgccgaaa ggcgatgggc actgatgatt accgttcttc
     5521 cctgtcccca ctgacgcagg ttatgcagga tctggtgctc tgtgcgtccg tccaccgccg
     5581 aaagcgcatc atcaaggatg aggatttccg cgttgactaa taacgctcga gcaatggaga
     5641 tacgctgttt ttgcccaccg gaaagcatca cgccgcgctc gcccacttct gtatcgtaac
     5701 cttgcggtag acgcaaaata tcgtcatgta cgctggctaa ccgcgcgaca tgctcaatct
     5761 cttgctgggt ggcattcggg caacccagcg cgatgttatt cgccacggtg tcagaaaaaa
     5821 ggaatggcgt ctggctgacc accgccaggc ggctacgcca gctatcgagt tgtaacttcg
     5881 tcagaggaat atcatgaaag cgaatatccc cctcgctgac gtcgaaatga cgctgaatga
     5941 gcgacaacag ggtacttttg ccggaaccag tcggcccgca aatacccagc atctgaccgg
     6001 gtttcagggc gaaattgacg ttttccagcg caggatggtc agtctgcgga tacgtgaact
     6061 ggtgaatatt tacatccagt tcgccacgcc cttccggcac gagttcgcta ccatcgttca
     6121 ccaccggcgc ttccgccagc atcgcgcgaa tacggctgta tgcagcacta ccacgttcca
     6181 caatgttaaa catccatgcc agcgccagca ttggccaaat catcagacct aaatacatca
     6241 taaaactggt gagctggccc agcgttaaac tgccctgcac caccatccaa ctaccgccgc
     6301 caatcgccag caagttcgcc ataccaatcg cgatatagat ggtcgggtcg aagcgagcat
     6361 caatacgcgc cacccgcatg ttttttttgc cggtatcttc ggcatccgcg gcaaataacg
     6421 ccgactggcg atcttccaga ccaaaggctt tgatcatgcg gatactggtg aggctttcct
     6481 gggtgcggtc attaagactg gaaaacgccg cctgtgccag cttaaagcgt tcatgcaagg
     6541 catcgccgtt gcgcttaatc atgatcgcca tcactggcat cggtaacagg gcaaataagg
     6601 tcaactgcca gctaatttgc gtagacatca taatcaacac agcgcagccc attaccagtg
     6661 aatccaccag cgtcagcacc ccttctccgg cggcaaacac gacgcgatcg acgtcattgg
     6721 tcgcacgagc catgagatca cccgtgcgat gacgcaggta aaactccggg tgctgccggc
     6781 ttagctgacg gtaataatct tcacgcagtt caacagccag ttggtaagac gcgccaaaca
     6841 gcaatacgcg ccagacataa cgcaggagat aaaccacaac agcaatcagc accatggtgg
     6901 cgatccacat caggatcagc ccggtagtaa agtgttgttc tgtcacgcca tcgacaacaa
     6961 taccaaccac ttttggcgga accagttgca gcatcgcgat aatgacaagc aaggcgacag
     7021 ccccaagata gcgacgccat tcccgacgga aataccagct taattgagca aataatcgca
     7081 cgcgttgtgt cctgacctga ttctggatat ttattcgatg ggtaaagaag tggtgtattt
     7141 aatctgttcc atcgcgaagc tggaagtgac gtccgacagc cccggcacgc tgtttaccag
     7201 acgcttataa aactcgtcgt agcgtttcat gtcggcaacc tagacgcgca tcagataatc
     7261 gtattcacca gccatgcgcc agaaccccag cacttctggc atttcggtaa ccaccgtgac
     7321 aaagcggcaa taccattcgc tgctgtgatg ttgcgttttt atcagcacaa aggcggtcag
     7381 gccgaggcct attttttccg gatccagcag ggcgactttg ccgataagga taccgtcgtc
     7441 ctccagccgt ttcaggcgct tccagcaggg ggtggttgtc agattaacgg cttcagccag
     7501 tgcctgcaaa gagagggtgc aatcctgctg cagtaaggcc agcagcttac ggtcaatttt
     7561 atctaacata gcccttccac ggagaatttt tttctctttt aattctattt taaaggtgaa
     7621 atcgcgacaa tttattctgt gcggtttaaa caccacggca caaaatgaca aattcaacgc
     7681 aaaaaaaaac cgggcaattg cccggtgctg gaagggatct cgttattcgg gggaataagg
     7741 tagatgtgga tagtccagcc agtgcgtcaa atagtgagag acagcctgat ttcggcaatg
     7801 tccaatcacc ggtaaatgcg ggagctccgc gcgcagttgc ggcatcgcat tgcccataat
     7861 aaacccgcta ccgacgctgc ctaacatttc gcgatcgttc atcgcatcac caaaggccat
     7921 gcaatcgcgc aacgataaac ctaaatgttg ggtcagcacc gtcaatgcag cgcctttatt
     7981 gcagcccacc ggcagcactt cgaggcaatc cgtggcggaa aaacacaaat gtgcacgctc
     8041 gcctaatgct tcgtatagct ggatctgcaa gcgtgtaaga tcgtcgtgat cgccacagaa
     8101 gcagatcttg gtgacgctgc tgagtggcat ttttttgaca tcgattatct gataacgaaa
     8161 accgctatag acaaatacct gcaacaacgc agggctctct ttcccggtaa accaaccgtc
     8221 gtcattgaag atatgcatgc tggctcgggt atcccattgc tgatacagca ccagctccgc
     8281 gacatccgca ggtaaatcat cacgatgtaa aagttcacct tccagagaat gcacgcgcgt
     8341 tccgttgccg gtaatcaaat atgcatccag cgatagcgcc ccgagaatat gctgcatctc
     8401 cagcgcatga cgccccgtgg caaaagtgag ggtaatgtcg cgttcacgca gtcgcgccaa
     8461 agtagagagg gttttctcac ctaaatgatg gtcaggcatc aataaagtgc catccatatc
     8521 aaatgctgcc agacgagcca tttctttctc cactctgtga cacggttaat ggtgattgag
     8581 tatcacctga tatatacgga agtaatagtg aatagttaaa taatattgtt ccgggtttat
     8641 atgcgattgc tcaaccgtct taaccagtat caacgtctgt ggcaaccttc agccggaaag
     8701 ccgcaaaccg tcaccgtcag cgaactggcc gaacgctgtt tttgcagcga acgccatgtt
     8761 cgtacgctgt tgcgtcaggc acaggaggcg ggatggctgg agtggcaggc gcagtcagga
     8821 cgcggaaagc gcggacaatt acgctttctg gtcacgccgg aatcgctacg caatgcgatg
     8881 atggaacagg cactggaaac cggaaagcag caagatgtgc tggagctggc gcaactggcc
     8941 ccaggtgagc tgcgcactct gttacagccg tttatgggcg gacaatggca aacgatacac
     9001 ccacgttgcg tattccctac tatcgcccgc tcgaaccgct acaaccaggc tttttgcccg
     9061 gccgtgccga gcagcatctc gccgggcaga tattttccgg cctgacccgc ttcgataata
     9121 atactcagcg cccgattggc gatttagcgc atcactggga aacctctact gacgggttac
     9181 gctgggactt ttatcttcgt tcaaccctac actggcataa cggcgatgca gtaaaagcct
     9241 cacacttaca ccagcgatta ttgatgctgt tacaactgcc agcactggat caattattta
     9301 ttagcgtgaa gcgtattgaa gtcacccatc cgcagtgtct gaccttcttt ttacatcgcc
     9361 ccgattactg gcttgcgcac cggctggcga gctattgcag ccatctggcg catccgcaat
     9421 tcccactgat cggcactggt ccttttcgct taacacaatt cacagcggag ctggtgcgcc
     9481 tggagagcca tgattattac catttgcgtc atccgctgct taaagcggtt gagtactgga
     9541 taactccgcc gcttttcgaa aaagatttgg gaaccagttg tcggcatccc gtgcaaatca
     9601 ccatcggcaa accggaggag ctgcaacggg tcagccaggt cagtagtggc atcagtttag
     9661 gtttttgcta tttaacgttg cgcaaaagtc cccgactctc cctctggcag gcgcgaaaag
     9721 tgatctccat tattcatcaa tccggtttat tacaaacgtt agaagtcgga gaaaacctga
     9781 tcaccgccag tcatgcatta ctgccaggct ggactattcc gcactggcaa gtaccggatg
     9841 aagtcaaact accgaaaacc ttgacgctgg tttatcacct accgatagaa cttcatacca
     9901 tggcagaacg cctacaggcg acactggcag cggaaggctg tgaactcaca attatttttc
     9961 ataacgcaaa aaactgggac gacacgaccc tactggcaca cgcagacctc atgatgggcg
    10021 acagattaat tggcgaagca ccggaatata ctctggagca atggctacgt tgcgatccac
    10081 tatggccaca tgttttcgac gctccagcat atgcacatct gcaatcgacg ctggacgcgg
    10141 cgcaagtaat gcctgatgag gaaaaccgat ttaatgccct gaaagcggtt tttagccagt
    10201 taatggcaga cgcgacgctg acgccgctgt tcaactatca ctatcgcatt agtgcccctc
    10261 ccggcgtgaa cggtgtgcga ctgacaccgc gcggctggtt tgaatttacc gaagcctggc
    10321 ttcccgcgcc gtcgcaatga atagctggtc cgggttaatc gcaggcgtta ccataacgct
    10381 tttattgttc aagcaggatt atctatgaaa cgtgctgtcg ttgtgttcag tggaggccag
    10441 gattccacca cctgtctggt gcaggcatta caacaatatg atgaagtcca ttgtgtgacg
    10501 ttcgattacg gtcagcgaca tcgcgcagaa atcgacgtgg cacgcgaact ggcgctgaaa
    10561 ctgggagcac gcgcgcataa ggttcttgat gtaacgctgc tcaacgagct ggcggtcagc
    10621 agcctgacac gtgacagcat tccggtgcct gattatgaac ctgaagccga tggcatcccg
    10681 aatacgtttg tcccagggcg taatattttg ttcctgacgc tggcggcaat atatgcgtat
    10741 caggtaaaag cagaagccgt aattaccggc gtctgtgaaa cggatttctc cggctacccg
    10801 gattgccgcg atgagtttgt gaaagcacta aaccatgccg tcagtttggg catggcgaaa
    10861 gatattcgtt ttgaaacgcc gctaatgtgg attgataaag cggaaacctg ggcgctggca
    10921 gattattacg gcaaactgga tttagtccgt aacgaaacgt tgacctgcta taacggcatt
    10981 aaaggcgacg gttgcggtca ttgtgcggca tgtaatttac gcgccaacgg tttgaatcat
    11041 tatctggccg ataaaccgac ggtgatggca gcgatgaagc agaaaaccgg gttgaggtaa
    11101 ttattccggg tgtcgccgga tgcggcttga gcatccggca ccacaaaacg tttacttaac
    11161 catctgctcc agcttttcgc gcaattcccc ttccagagct aatgctttct gcgttttaag
    11221 atcaatacaa acaaacgtaa taagcgcatc cgctaccacc tgcccttccg gctccagtgt
    11281 aatgacctag cttaagatgc cgcttttacc gtttaattgc tgcaactgac tggtaatagt
    11341 taacaggtcg cttaataccg ctgggcgacg atagttaata ttgatattga ccacgacgaa
    11401 ggcgatgtta tgggccgtca tccactgaaa actgtcgcta ttttccaacc catcccagcg
    11461 ggcttcttcg agaaattcaa ggtagcgggc gttgttgacg tgctggtaaa cgtcgagatg
    11521 atatccacga actttgattt gtgtttgcat agcgcaataa ccttacgttg ttgttataag
    11581 tacagaggtc ataactggta tgacctcttc agtctggcaa aaaattgcca ctatgcaaat
    11641 taattacagg gttaataccg ccagattacg ttccaccagc gaattgccca tccccggcac
    11701 ctgctttaga tcctccaccg ttttaaacgg accgtactct tcacgataac tgacaatcgc
    11761 ctgcgctttc ttcaggccaa cgccattcat cgcgcgggct agctcttccg cgctggcatt
    11821 attaatgctg acccgggtgc cttcttcgtc actggctttc gccggtactg ctgctttact
    11881 ttgtgctgca ggagcttccg ctttggtttc taccgccgtc ggtttcgcca cagaagctgc
    11941 cgccagcgcg ctatgagaca ttccggcaca ggccagggac agggtaatga gcagtgcttt
    12001 aattccgtgt ttcatactgt tttctccttg tttgttaaca gtggaatcac cttaacggga
    12061 gtgaaaacag taaacaaatg gcaatgttca gaaatggaaa aggccgcgaa agcgaccttt
    12121 tcttgttaca gctgcaacgc atttttttgc gaggcggctt caggattatt gctgttccag
    12181 cgcatcgcca attttgattt tcgcctcttt acgcaggtta ctcatcagag cttcaaagac
    12241 gatttgtgcg ttgttctggg tgataccctg caccatcgct tttttctgat cttccggcat
    12301 tgaaccttgt ttcacttcat ccagcgccag cagaaccaca ttaccttgca tatcggtcgc
    12361 cataccgtag ctcggtttgt ctttcgctgg cagtggcagt gcaaacgccg cctggctaat
    12421 cgggtcacga ccggaacggc ttaaggtttt cggctcgcca aatttcagac cggcagcctg
    12481 catagcttcc gcacctttgc cggctttcaa atcaaccagc agtttctcag catccacttt
    12541 cgcctgttgt tcagctttgt tgtgctgaac cagtgcctta acttgttcct gaacatctgc
    12601 caacggtttc accgcttccg gtttgtgctc gctgacgcgc agcacgaatg cgcggtcgcc
    12661 gtctacggtg atgatgtcag agttgatgcc cgacgcgccg ttttcaccta ccagaccgcc
    12721 gttaaagata gcgtcggcaa ccggcttgaa gttcaactct tccggcaggt tatctttgct
    12781 gaaccaaccc gtctgagtgg ctttaacgcc ggcagcttgc tctgcaccgg ccagagactc
    12841 ggtttcgttg cttgccgcat cgctcacttt ctgctgcagc gcatagtacg catcgagggc
    12901 tttttcgtgt ttcactttcg ccgcaatgtc gtcacgtact tcgtctaacg atttcacttt
    12961 cgctggctga atgtcgtcca gacgtacaat caggaaaccg accgaagatt tgatgacacc
    13021 agacagttgg cctttttctt tcagaccagc atttttcagt tcgtcgggga tagtggcatc
    13081 ttctaaccaa cccatatcgc cgccgttacg ggcagagata atatcggcag atttttcttt
    13141 ggctaatgca gcaaaatcac cgcctttatt cagctcatca agtaccgctt tcgcttcatc
    13201 ttcagttttg gtctggatga tgctgtagcg ggtacgctgc ggctgggtga attgatcctg
    13261 atgctgatcg tagtagctct ggatatccgc atcgctaacc ggttgctgca tcgttgcggc
    13321 aaccagcttg atgtaactca cgcggaattg ttccggcgtc atgaaattgt ttttgttttg
    13381 ttcgtagtag ctggcaatct cctgttcggt cacaggctgc ttcgccgcca gcgcgttaac
    13441 atcgatagtc gcctcacgca ccacgcgttg ttgcgcgacc agtgccgcca gctcgtcggt
    13501 ttcacctttc agcataaaat cggtaccggc aacgccgtta atcagctgtt gggtagtgag
    13561 ctggttacgc agcgcctggg cgtactgatc ggcggtcatc cccatctggt tgaggatacc
    13621 gttatagcgg ctgttatcaa atttgccgtc aacctggaag gctggggtcg cgaaaatcgc
    13681 ctgtttaacc tgctcatcac tgatacccag tttcagctca cgagcgtact gatccaacag
    13741 cgcctcgtcg atcagacgat tcagcacctg ttgacgcagg gttttcatat agccttcgtt
    13801 cgctgccagc tcggagtatt gatcgcccag ctgttgctgc atgcgattac gctcgctgtt
    13861 aaaggcgttc tcgaactgcc cacggctgat ttcctggtca ttcacttttg cggcgtagtt
    13921 attgcctccg ccaatcaggt aaccactcac gccggtcaat atgaacgaca cgataatgat
    13981 accgaaaata atcttgagca cgagactgtt tgcagccgtg cgtaagctgt ccatcatggt
    14041 gtaacaacac tccgctgtag atgactgtgt acctcacgca acgtatcgat gcgcgcaaag
    14101 gcctattgtg acaagaaacg ggggcaattg tcagcccaca agacacagaa agtcaccgaa
    14161 tacaaataaa aaaggcacat cagtagatgc gcccttgaac ttcgtcacat ccccactggg
    14221 gacaacgctt agtttaccgc gtctttcagt gctttacctg cacggaagct cggtacttta
    14281 gcggcagcga tggtgatctc tttaccggtc tgcgggttgc ggccagtacg ggcagcacgc
    14341 tctttaacgg caaaagtacc aaaacctacc agtgctacat catccccttc tttcagagat
    14401 tcagttacgg aagcaataat agcatctaac gcacggccag ccgcagcttt agagatatca
    14461 gcccctgcag caatcttgtc gatcaattga gatttattca ctcttctctt cctctttata
    14521 atttatatcg cacttgaatc cttcaaggta cgaacgcgca gcagttatat caggccagcc
    14581 atccccttac aacacccctt aataagggca agcccgatcc gctatctaac ttagcgagac
    14641 aaaaaaaggc tggcaagccc gaattagcct gccagccctg tttttattag tgcattttgc
    14701 gcgaggtcac tattttgcag tcacaacctg catgccagac ggttcatttt gcagcgccag
    14761 agtcagaact tcctcaatgc gcttcacagg atgaatgtcc agatcggcaa ttacgttgtc
    14821 aggaatctct tccagatcgc gtttattttc gaacggaatt agcactgttt taatcccgcc
    14881 gcgatgcgct gccaggagtt tttctttcaa accaccgatc ggcagtacct gaccacgcag
    14941 agtgatctca ccggtcattg ccacatcggc acgaaccggg ttaccggtca ggcaagaaac
    15001 cagcgcggtg cacatagcaa taccggcact cggaccatct ttcggcgtcg caccttccgg
    15061 tacgtggacg tggatgtcac gtttttcgta aaaatcaggg ttgatcccca gtttttccgc
    15121 acgcgcacga accaccgtta acgccgcctg aatagactcc tgcatcactt cgccgagcga
    15181 accggtatag gtcagtttgc ctttacccgg aacacatgcg gtttcaatgg tcagcaagtc
    15241 accgcccact tccgtccacg ccagaccggt tacctgaccg acacggtttt cgttatcagc
    15301 gcgaccatag tcgaaacgct gaacaccgag gtagtcatgc aggttatcgc cgttaatttc
    15361 gatatgtttt aatgacttat cgagcagtaa ctgcttaacc gctttgcggc acagtttgga
    15421 gatttcacgc tccagaccac gcacgcccgc ctcacgggtg tagtaacgaa taatgccgat
    15481 aatggcgcta tcgtcgacgg tcagctcacc ttttttcagt gcattacgtt caatctgctt
    15541 cggcagcagg tgacgtttgg cgatgttcag tttttcatct tcggtataac cggagaggcg
    15601 aatcacttcc atacgatcca gcagcggtgc cggaatgttc atggagttcg acgtcgcgac
    15661 aaacatcacg tcgctgagat cgtaatccac ttccaggtag tggtcgctga acgctacgtt
    15721 ctgctctgga tccagcactt caagcagtgc agaggccgga tcgcctcgca tgtcagaaga
    15781 cattttgtcg atctcatcga gcaggaacag cgggtttttc acgcccactt tcgccatttt
    15841 ctggatcagt ttacccggca tagaaccgat gtaagtacgg cggtgaccac ggatttccgc
    15901 ttcatcacgc acgccgccca gcgccatacg gacatattta cgcccggtgg ctttggcaat
    15961 ggactgacca agagaggttt tacctacccc cggcggccct accaggcaga ggatcggtcc
    16021 cttgattttg ttgacacggc tttgaaccgc aaggtactca aggatgcgat ctttcacgcg
    16081 ctccagacca taatggtcgg tatcaaggat ttcctgcgcc tgacgcaggt cttttttgac
    16141 cttgctgcgc gcattccacg gtacctgtac catccagtcg atataaccac gcactacggt
    16201 cgcttctgcc gacatcggag acatcatttt cagcttctgc aactctgctt ccgctttctc
    16261 ttttgcctct ttcggcatct tcgccgcgtc gattttgcgc ttcagggctt cgttttcgtc
    16321 cggcgcgtcg tccatttcac cgagttcttt ctgaatagct ttcatttgct cgttcagata
    16381 gtactcacgc tgggatttct ccatctgctt tttaacgcgg ttgcgaatgc gtttctcaac
    16441 ctgcagcaga tcgatttccg attccatcat tgccatcaga tattccagac gttcgttaac
    16501 gtcggacatc tccagaacgg actgtttgtc agccagtttc agcggcatat gtgcagcaat
    16561 ggtatccgcc agacgcgccg gatcgtcgat gctattcagc gacgtcagca cttctggtgg
    16621 gatttttttg ttcagcttga tgtagccttc gaactggctg attgcagtac gcaccagcac
    16681 ttcctgttcc cgctcatcaa tggtcggcga ctccagatac tccgccttcg cagaaaagtg
    16741 ttcgccattg tcagagagcg cagaaatacg cgcgcgctgt aacccctcga ccagcacttt
    16801 gactgtgccg tcaggcagtt tcagcatctg caatatagag gccacggtcc cgacggtgaa
    16861 aagatcgttt acacccggct catccgttga agcttctttc tgcgcgacca gcataatttt
    16921 tttatcatgg tccatcgccg cttccagaca acggatagat ttttcccgcc cgacaaataa
    16981 ggggatgacc atgtgcggat aaaccaccac atcgcgcagc ggcaatacgg ggatttcaat
    17041 gcgttcagaa cgctcaggat tcatagagct ctctcttagt ttaatttccg ccaggtaatc
    17101 agatgacacg actgtgcttc acgccatcta ttaacatgta cgtcagtata tggggatgtt
    17161 tcccccacat tcaacgccga gaatagagga aaaattaaag gggagataaa atcccccctt
    17221 tttgtttaac taattgtatg ggaatggtta attattcacc agatgcctgt tgcgcttccg
    17281 gcttaccata aatcagcaac ggtttgcttt gaccatcaat taccgactcg tcaataacca
    17341 ctttttcgac gtcttccatg gacggcagat cgtacatggt atcgagcagt gcggcttcta
    17401 cgatggaacg caggccacgg gcaccggttt tacgcgccat cgctttctta gcgatagcat
    17461 ccagcgcctc gtcacggaat tccagatcca cgccttccag attaaacagc gcctgatact
    17521 gcttggtcag ggcgtttttc ggctctttga ggatctgaat cagagcttct tcgctcagtt
    17581 cattcaacgt tgctacaacc ggcagacgac caatgaactc agggataaga ccaaacttga
    17641 tcagatcttc cggttcaacc tgcgccagca gctcgccttc gcttgctttg tcggacttcg
    17701 cttttaccgt cgcgccaaaa ccaatgccgg agccggtttc tacacggtgg gaaatcactt
    17761 tatccagacc ggcaaacgca ccgccacaaa taaacaggat cttagaggta tcaacctgca
    17821 agaattcctg ctgcggatgt ttacgcccac cttgcggtgg aacagcagct accgtacctt
    17881 cgatcagttt caacagtgcc tgctgtactc cttcaccgga aacgtctcgg gtaatggacg
    17941 ggttgtctga cttacgagaa atcttgtcga tttcatcgat gtagacaatc ccgcgctgcg
    18001 ctttctgtac gtcgtaatcg cacttttgca acagcttctg aatgatgttt tcaacgtctt
    18061 cgcccacata accggcttcg gtcagcgtgg ttgcgtcggc catggtgaac gggacgtcca
    18121 gcaggcgcgc cagcgtttca gccagcagcg ttttaccgga accggtcgga ccgatcagca
    18181 gaatgttact tttgtccaac tcgacaccat tgctggtatc gccgttgcgc agacgtttgt
    18241 aatggttgta taccgcgacc gccagcactt ttttcgcctg ttcctggccg ataacgtaat
    18301 cgtccaggtg gttgcgaatt tcatgcggcg tcggtagcgc actgcgttca cgatgcggtg
    18361 caacttcttt aatctcttcg cgaatgatgt cgttacataa atcaacacat tcgtcgcaga
    18421 tatacacgga tggaccggca atcagcttgc gcacttcatg ctggcttttg ccgcaaaaag
    18481 agcaatacag caatttgcct gagccatctt tgcgtttatc tgtcatgggt caaaacctct
    18541 tctttgttct ttgtgccgca cacgacgacg caaatgccat tctcaggcgc aagccgctta
    18601 cagcgttgtg ccgccctaga taagtatagc ggcacagttg cgcctctggc atcaattacg
    18661 atgggtcaga atcgaatcga ccagaccgta ttccaccgct tcaggggcgg aaaggaagcg
    18721 atcgcgctcg gtatcacgtt caatctgttc taatgattga cccgtatgaa gcgccataag
    18781 ttcattcatg cgccctttaa ctttcagaat ttcacgagca tgaatttcga tatcggtcgc
    18841 ctggccctgg tagccgccca acggttggtg aatcatcacg cgcgaattcg gcaggcaaaa
    18901 acgtttacct tttgcccctg cggtcaacaa gaaagcgccc atcgaggccg cctggcccat
    18961 acagatggtg ctgacatcag gcttgataaa ctgcatggtg tcatagatag acatcccggc
    19021 agtgatcacc ccgcctgggg agttaatgta cagatagata tctttttctg ggttttccgc
    19081 ttccaggaac agcatctgcg ccacaatcag gttagccatg tggtcttcaa cctggccagt
    19141 cagaaaaatg acgcgttcct taagtagacg agaatagata tcaaaagagc gctcaccgcg
    19201 tgaggtctgt tcaatgacca tcggcaccag cgccatatgg ggtgcaaagt tatctcgttc
    19261 gccgctgtat gacatttccg tctcctggat aaaattgaaa aaaacctgct gtaccgattg
    19321 taaccttatg gacggattat cagctagtcc ctgtgttatg ggtacggcat caccatattt
    19381 caagcataac aatcttttgt tgttacgcta acaccgaaag aggcttttcg cacggctcca
    19441 tgcaaaattc atgacaaaaa aaagcccacc acctggcggt gacgggcctt tgtgcgaatt
    19501 tagcgcgtta tgctgcgtaa attacgcctg ctggttcatc agctcgttga aagtggtttc
    19561 tttttcagtc actttcgctt tcgccagtac agcttcaaca gcctgttctt ccagagcaac
    19621 attgcgcatg ttgtccatca gttctttgtt tttgctgtag aactcgataa cttctttcgg
    19681 atcttcgtac gcagaagcca tctcttcgat caggcctttc acgcgctctt cgtcagcttt
    19741 cagctcgttg gtgcggataa cttcgcccag cagcaggcca acaactacgc ggcgtttagc
    19801 ctgttcttcg aacagttcgc gcggcagttc cagagcttgt ttttcgttgc caccgaaacg
    19861 ctgtgcagcc tggcgacgca gaacgtcgat ttcgctgtcg atcagcgcag ccggtacgtc
    19921 gatgtcgtta gcttttacca gaccttcgat cgcctgagac ttaacgcggt tacggatggc
    19981 gctcttcagc tcgcgctcca tgtttttacg cacttcagcg cgcagacctt ctacggaacc
    20041 atcttcaacg ccgaaacgtt tgatgaattc tgcagtcagt tccggcagtt cacgctcttc
    20101 aactttcttc aggttgatag cgaatttcgc tgctttacct ttcaggtttt ctgcgtggta
    20161 ttcttccggg aaggtcacgt cgatggtgaa ctcttcgcca gctttgtggc ctttgatacc
    20221 gtcttcaaag cccgggatca tacgaccctg gcccatcgcc agtacgaaat cagacgcttt
    20281 accgccttcg aactcttcgc cgtctacaga accggtgaag tcgatggtta cgcggtcttc
    20341 tgcttcaaca gcgccgtctt tttctttcca ggtcgcctgc tgtttacgca gagtatccag
    20401 catgccgtca acgtcagcgt cggtcacttc aacgatcggt ttttcaactt cgatcgcttc
    20461 cagaccctgc agttcaactt ccggataaac ttcaaactct acagagtaag tgaagtcttc
    20521 acccagcttg tattcgcccg gaacataagt cggtgcgcca gccggattga ttttttcttt
    20581 aatgatggcg tcaatgaagt tacggctcat caggtcaccc agaacgtcct ggcgtacaga
    20641 cgcgccataa cgctgagcaa cgatattcat tggcactttg cctttgcgga agccgtcaat
    20701 acgtactttt ttcgcaacgt tgaccagctc gcttttaaca gcggtctcga tgctgtcagc
    20761 agcgatagta atcgttacac ggcggccaag gccttgagtg gtttcaactg aaacttgcat
    20821 cttgttacct caaaaaatca cagtgctcgg tcaactctta ccccgcaagc acaggttgtt
    20881 ggcttcgcgg aaccgggatg ttcttataat caatcacatt ccctgttacc agaatcatcc
    20941 cgaaagcatt caaataggac gcggcattat agcggcatca cttttatgag tcgagaacgg
    21001 ttattgcgcg ctgtcgcgta ttttttcaca attcccgggc aattttcgtc tgaatgctgg
    21061 gcaaaaagcg caaaatccag acaaaacaaa acggcccgca ggccgttttt cataaaatct
    21121 ttagcaacat actggaaaag cgccgacagt tgcaaatgcg tttttacgcg atgcttcctg
    21181 ctccacgaca gggaggagag gcaaagacgg tgtcctgcaa cccttcccac tccttaatag
    21241 tgtaagtatg cagagccagc gcatgaacgg tagtagagag ttcctccgct aaagtactgt
    21301 aaatcattcg atgacgattc agaaaacgtt cacccgtaaa acgatcgctg accagcacaa
    21361 ctttaaaatg gctttcagag ccggctggga cattgtgacg atagctttca tccactactt
    21421 cgaggaatac gggttggaac gccgccctta atttttcttc tatccgctca cgtatcatca
    21481 tgaaatatct cctccgacaa cgctgagatg tcatccatcc ctttaaatac tagccgcttt
    21541 taccgtttcc attgcagctt aacaacaaat atttaggttt cgtcctggtt tttccgtcaa
    21601 gcgtatgaag tttactgatt taaagattga aacacaggcc tcgcatcagc gtttctttta
    21661 ccaggggtga ttatttcgtc aacatgatga atttacatgc cttacccact tcccctcgcc
    21721 gttggcaatg ttatgatggc gggaattttt accaaacacg cttaagagtc tctgagagcc
    21781 cgaacatgtt caaaaaaatc ctcttcccgt tagttgctct gtttatgctt gcaggatgcg
    21841 caaaaccgcc aacaactatt gaagtttccc cgacgattac actgccacag caggatccaa
    21901 gcctgatggg cgtcaccgta agcattaatg gtgccgatca gcgtacagat caggcgctgg
    21961 caaaagtcac ccgcgataat caaatcgtta ccctgaccgc ctcccgcgat ctgcgtttcc
    22021 tgctgcaaga agtgctggaa aaacagatga ccgcgcgtgg ttacatggtt ggtccgaatg
    22081 gcccggttaa tctgcaaatc atcgttagcc aactgtatgc tgacgtgtcc cagggcaacg
    22141 tgcgctacaa catcgctacc aaagcggata tcgccatcat cgctaccgcg cagaatggca
    22201 acaaaatgac caaaaactat cgtgccagct acaacgttga aggtgcgttc caggcctcca
    22261 acaaaaatat cgccgatgcg gttaacagtg tgctgaccga taccatcgct gatatgtctc
    22321 aggacaccag cattcacgaa ttcatcaagc agaacgcgcg ttaatttctg ccctctggcc
    22381 cggtgcaagc cgggcctgta gacgcccatg tccagtcaat atttacgtat ttttcaacag
    22441 ccgcgttcag ccatattgct gatcctgggt tttgcttccg ggctaccgct cgccctgaca
    22501 tccggcacct tacaggcctg gatgacggtc gaaaatatcg atctcaaaac cattggtttc
    22561 ttctctctgg taggccaggc ttacgttttt aaattcctct ggtcaccgct gatggaccgc
    22621 tacacgcccc cattttttgg gcggcggcgc ggttggctgc tcgccacgca aatcctgtta
    22681 ttagtcgcca ttgcggcgat gggttttctc gaaccaggca cccaactccg ctggatggcg
    22741 gcgctggcgg tggtgatcgc tttttgctct gcctcccagg atattgtctt cgatgcgtgg
    22801 aaaaccgatg tgctcccggc agaagaacgt ggtgcgggcg cggcaatcag cgtgctgggt
    22861 taccgtttag ggatgctggt ttccggcggc ctagccctgt ggctggcaga taaatggttg
    22921 ggctggcagg gcatgtactg gttgatggcg gcgctgttga tcccctgtat tatcgcgacg
    22981 ttgcttgcac cagaaccaac cgacaccatt cctgtgccca aaacgctgga acaagcggtt
    23041 gttgcacctc tgcgagattt ctttggtcgc aataatgcct ggcttatttt gcttcttatc
    23101 gtgctgtata agctgggcga cgcattcgcc atgagcctga caaccacgtt tttgattcgc
    23161 ggcgtcgggt ttgatgcggg tgaagtaggc gtggtaaaca aaacgcttgg cttactggcg
    23221 accattgttg gcgcattgta cggtgggatt ttgatgcagc gcctgtcgct gttccgggcg
    23281 ctgctgattt tcggcatttt acaaggtgcg tctaacgctg gttactggct gctgtcgatt
    23341 actgataagc atctctacag catgggcgca gccgtctttt tcgaaaacct ctgtggcggg
    23401 atgggcacat cagcctttgt cgcgctgtta atgacgctgt gtaataagtc attttccgct
    23461 actcaatttg ccctgctctc agcgctttcc gctgtagggc gagtttatgt cggccccgtg
    23521 gcgggttggt ttgttgaagc acacggctgg tcgacattct atctattctc cgtcgccgct
    23581 gccgtaccgg ggcttatttt gctgctggtt tgccgccaga cgcttgaata tacacgagta
    23641 aatgacaact ttatctcccg taccgaatat ccggcaggtt atgcctttgc catgtggaca
    23701 ctggcggcgg gcgtcagcct gttggccgtg tggttacttc tgttgacgat ggacgcgctg
    23761 gatttgacgc acttctcttt cctgcctgct ctgctggaag tcggggtttt agtcgccctt
    23821 tctggcgtcg tgcttggtgg tttgctggat tatctggcgc tacgaaaaac gcatctgacg
    23881 taatctgtaa atattattta gctgtagtaa tcactcgccg taattattac ggctaaataa
    23941 acatcagcat cgcttattaa tatttgttct tttgtgcggc ttagc
//
LOCUS       DAJTYG010000056        23938 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTYG010000056 DAJTYG010000000
VERSION     DAJTYG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23938
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.056"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..178)
                     /locus_tag="OO568_002407"
     CDS             complement(<1..178)
                     /locus_tag="OO568_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641731.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLS"
     gene            249..314
                     /gene="yobI"
                     /locus_tag="OO568_002408"
     CDS             249..314
                     /gene="yobI"
                     /locus_tag="OO568_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6641732.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            453..1064
                     /gene="ruvA"
                     /locus_tag="OO568_002409"
     CDS             453..1064
                     /gene="ruvA"
                     /locus_tag="OO568_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6641733.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            1073..2083
                     /gene="ruvB"
                     /locus_tag="OO568_002410"
     CDS             1073..2083
                     /gene="ruvB"
                     /locus_tag="OO568_002410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6641734.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(2230..3015)
                     /gene="znuB"
                     /locus_tag="OO568_002411"
     CDS             complement(2230..3015)
                     /gene="znuB"
                     /locus_tag="OO568_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6641735.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(3012..3767)
                     /gene="znuC"
                     /locus_tag="OO568_002412"
     CDS             complement(3012..3767)
                     /gene="znuC"
                     /locus_tag="OO568_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6641736.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3846..4778
                     /gene="znuA"
                     /locus_tag="OO568_002413"
     CDS             3846..4778
                     /gene="znuA"
                     /locus_tag="OO568_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6641737.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4794..6116
                     /gene="mepM"
                     /locus_tag="OO568_002414"
     CDS             4794..6116
                     /gene="mepM"
                     /locus_tag="OO568_002414"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6641738.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            6236..7207
                     /gene="lpxM"
                     /locus_tag="OO568_002415"
                     /gene_synonym="msbB"
     CDS             6236..7207
                     /gene="lpxM"
                     /locus_tag="OO568_002415"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6641739.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7338..8780)
                     /gene="pyk"
                     /locus_tag="OO568_002416"
     CDS             complement(7338..8780)
                     /gene="pyk"
                     /locus_tag="OO568_002416"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6641740.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMHADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(8908..9777)
                     /gene="hexR"
                     /locus_tag="OO568_002417"
                     /gene_synonym="yebK"
     CDS             complement(8908..9777)
                     /gene="hexR"
                     /locus_tag="OO568_002417"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6641741.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            10115..11590
                     /gene="zwf"
                     /locus_tag="OO568_002418"
     CDS             10115..11590
                     /gene="zwf"
                     /locus_tag="OO568_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6641742.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11825..13636
                     /gene="edd"
                     /locus_tag="OO568_002419"
     CDS             11825..13636
                     /gene="edd"
                     /locus_tag="OO568_002419"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6641743.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLFRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVRTGRELFSALREKLSGAEQGATCITF"
     gene            13673..14314
                     /gene="kdgA"
                     /locus_tag="OO568_002420"
                     /gene_synonym="eda"
     CDS             13673..14314
                     /gene="kdgA"
                     /locus_tag="OO568_002420"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6641744.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(14369..15547)
                     /gene="purT"
                     /locus_tag="OO568_002421"
     CDS             complement(14369..15547)
                     /gene="purT"
                     /locus_tag="OO568_002421"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6641745.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            15681..15971
                     /gene="yebG"
                     /locus_tag="OO568_002422"
                     /pseudo
     CDS             15681..15971
                     /gene="yebG"
                     /locus_tag="OO568_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            16038..16394
                     /locus_tag="OO568_002423"
     CDS             16038..16394
                     /locus_tag="OO568_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6641746.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDATGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            16721..17380
                     /locus_tag="OO568_002424"
     CDS             16721..17380
                     /locus_tag="OO568_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6641747.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            17589..19649
                     /gene="ptrB"
                     /locus_tag="OO568_002425"
     CDS             17589..19649
                     /gene="ptrB"
                     /locus_tag="OO568_002425"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6641748.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQHWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPT
                     TPAD"
     gene            complement(19646..20308)
                     /gene="exoX"
                     /locus_tag="OO568_002426"
     CDS             complement(19646..20308)
                     /gene="exoX"
                     /locus_tag="OO568_002426"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6641749.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(20332..20988)
                     /locus_tag="OO568_002427"
     CDS             complement(20332..20988)
                     /locus_tag="OO568_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR6641750.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGNYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(21090..21320)
                     /gene="holE"
                     /locus_tag="OO568_002428"
     CDS             complement(21090..21320)
                     /gene="holE"
                     /locus_tag="OO568_002428"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6641751.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            21459..21833
                     /gene="yobA"
                     /locus_tag="OO568_002429"
     CDS             21459..21833
                     /gene="yobA"
                     /locus_tag="OO568_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6641752.1"
                     /translation="MASTARSLRYALTILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            21837..22709
                     /gene="copD"
                     /locus_tag="OO568_002430"
                     /pseudo
     CDS             21837..22709
                     /gene="copD"
                     /locus_tag="OO568_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            22722..23063
                     /locus_tag="OO568_002431"
     CDS             22722..23063
                     /locus_tag="OO568_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6641753.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(23456..>23938)
                     /locus_tag="OO568_002432"
     CDS             complement(23456..>23938)
                     /locus_tag="OO568_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6641754.1"
                     /translation="FPLAMDLALVTGQRREDITNMRFSDIYDDRLHIRQIKTGMMIAI
                     PLSLSLPVAGLRLGAVVEQCRMVSRGDYLISAGIRKNSPDGSIHPDGLTKKFVAARKL
                     TGILFSENPPTFHEIRSLAGRLYKEICGEEFAQRLLGHTSEKTTKMYLDEREKTYLLL
                     "
BASE COUNT         6088 a         5906 c         6108 g         5836 t
ORIGIN      
        1 ttgaaagggg aacccggctt tctgcaacca ggaagtcttc accgttatga ccgataatga
       61 tcccgacgtg attactccag caatttgatg cagctgatat ttgaccaaat aaggcagcac
      121 ctatacatgt aaagacgata tctccaattt catattcagc agggtaatta atattcacgt
      181 tgtcattcca ttgaaataga tacgaatata cgtaatagca taaaaaaatt attttttcag
      241 gagtcgcaat gtatattttt attacgcatt tcttcactga atatgtaata ttcaaatatt
      301 tgcttccaat ataacctgtt gaataaatta tactgtgcca tttttcagat catcgggaca
      361 cctcgcaagt tttcttcatc ctacgctgga tatctatcca gcattttttt atcatacagc
      421 attatctttg attcattacg caggagcgtc atgtgatagg cagactcaga ggcatcatca
      481 ttgaaaaaca acccccgctg gtgttaattg aagtgggcgg cgtaggctat gaagtgcata
      541 tgccgatgac ctgtttttat gaactccctg aagcgggtca ggaagcgatc gttttcaccc
      601 actttgtggt gcgtgaagac gcgcaactgc tgtacggttt taacaataaa caagaacgca
      661 cgttgttcaa agagttgatc aaaaccaacg gcgtcggccc gaagttggcc ctggcgatcc
      721 tctccggaat gtcagcgcag cagttcgtta atgccgttga gcgtgaagaa gtgggggcac
      781 tggtgaaact gccgggtatt ggcaaaaaaa ccgccgaacg cttgattgtt gaaatgaaag
      841 accgatttaa aggtttgcat ggcgatctct ttacgccagc cgccgacctg gtactcacgt
      901 ctcctgccag cccggcgacc gacgatgctg aacaagaagc agttgccgcg ctggtggcgc
      961 taggctataa accacaagaa gcaagccgca tggtgagcaa aatcgctcgc cctgacgcca
     1021 gcagtgaaac attaattcgc gaagccctac gcgccgcgtt atgaggtaaa ggatgattga
     1081 agcagaccgt ctgatttctg ccggtaccac tttgccggaa gatgtagcag atcgcgccat
     1141 tcgccctaaa ttactggaag agtatgttgg tcagccgcag gttcgttcac agatggagat
     1201 tttcatcaaa gcagcgaaac tgcgcggcga tgccctcgat catttgttga tttttggtcc
     1261 tccggggttg ggtaaaacta cgcttgccaa cattgtcgcc aatgaaatgg gcgttaattt
     1321 acgcacgact tctggtccgg tgctggaaaa ggcgggcgat ttggctgcga tgctcactaa
     1381 ccttgaaccg catgacgtgc tgtttattga tgagatccac cgtttatcgc cagttgttga
     1441 agaagtgctg tacccggcaa tggaagacta ccaactggat atcatgattg gtgaaggtcc
     1501 ggcggcacgc tccattaaaa ttgatttgcc gccgtttacc ctgattggcg caaccacgcg
     1561 cgcaggttcg ctgacatcac cgttgcgcga ccgttttggt attgtgcaac gtctggagtt
     1621 ttatcaggtg ccggatctgc aatatatcgt cagtcgcagt gcacgcttta tggggcttga
     1681 gatgagtgat gacggtgcgc aggaagtagc tcgtcgcgct cgcggtacgc cacgtattgc
     1741 caaccgtctg ctgcgtcgag tgcgtgattt cgccgaagtg aagcacgatg gcaccatctc
     1801 ggcagatatc gctgctcagg cgctggatat gttgaatgtc gatgctgaag gtttcgatta
     1861 tatggaccgc aaattgttgc tggcggtaat cgataagttc tttggtgggc cggtaggtct
     1921 ggataacctg gcggcagcca ttggcgaaga acgtgaaacc attgaagatg tactggaacc
     1981 ttatttgatt cagcaaggct ttttgcagcg tacaccgcgc gggcgtatgg cgacgacgcg
     2041 ggcgtggaat cactttggca taacgccgcc agaaatgccg taagtcggat tggtcgctcg
     2101 cgccgcatcc gacactggca gtgtgtctga tgcgacactg ttcgcgtctt atcaggccag
     2161 ccagagatag cgaaccgtag gtcggataag gcgctcgcgc cgcatccgac aaatgtgttc
     2221 agcgatagat tagctggcct gctttttcat catactgata ataaataaca gtgccgcaca
     2281 tagcaccacc gacggacccg ccggcgtatc gtaaaacgcg gaaaaggtta aaccgccagt
     2341 tactgccacc atccccacca aaacagcgac accagccatc tgttccggcg tacgggcaaa
     2401 gcgacgcgca gtagctgcag gaataatcag caacgaagta ataatcaacg caccgacaaa
     2461 tttcatcgct acaccaatcg tcaatgccgt caccagcatc aacaacaatt tcacttgctg
     2521 taatttcaca ccatcaacaa acgccagatc cgggctaatc gtcatcgaca gcaaattgcg
     2581 ccattgccag aacaaaatag ccaccacgat gaccacgcca atcgcaatag agatgagatc
     2641 ttctggcgtc actgccagta aatcaccgaa caggtaagcc atcaaatcaa cacgaatgtt
     2701 agacatcaga ctaacgacca ctaggcccag cgacagggca ctgtgcgcca taatccctaa
     2761 taacgtgtcg atcgccagct gtggacgctt ctccagccat accagaccgc ccgccagcag
     2821 cagcgtaacg gcaatcaccg catagaatgg attcacgtcc agcaacaaac caaacgcgac
     2881 accaagtaat gaggcatgag ccagcgtatc accgaaataa gacatacgac gccagactac
     2941 aaacgaaccc agcggacccg cggcacaggc gagcatgatc ccggctaacc aaccgggaaa
     3001 taataattca atcatgagcg atcatttccc cgacgcaaaa caattcgtcc ctgtaaatcg
     3061 tgacgatgat tatgatgatg gcgatagata cccagttgtt cagcaccacg aggaccaaac
     3121 attgaaataa actccggatg cagggaaaca acttccggtg tgccggaaca acaaatgtgg
     3181 tgattcaggc acagcacttc atcggttttt gccatcacca gatgcagatc gtgagaaacc
     3241 attaaaacgc cacaatccag ttcacgacgc aactggtcaa taaggtcata taacgccact
     3301 tggccattta catccacgcc ttgagtgggt tcatccagca ctaataattg cgggcaattt
     3361 aacaatgctc gcgctaatag tacacgctgc gtttcgccac cagagagctt ttgcatcggt
     3421 gcgttaatca ggtgcccggc ctggacacgt ttcagtgcag gcaaaatatc ttctttatgt
     3481 gtaccagggc gtaagcgtaa aaaacggttt acggtcagtg gcaacgtggt gtcgagatac
     3541 agcttctgcg gtacatagcc gatgcgcagt tttccgttgc gcttgataac cccttcatcg
     3601 ggtgttacca gcccgagcac tacccgtacc agtgtcgact tacctgcgcc atttggccca
     3661 agtaaagtca aaatttttcc aggtttaagt tccagcgaca catcagagag gacgcggcgt
     3721 tggccaaaag aaaccgagac attttccagg gaaaccagac ttgtcatgtt aattttagtc
     3781 ttgcagtagt catgaaatgt tataatatca cacttctcat attcattacg atgattggtc
     3841 gcattatgtt acataaaaaa acgcttcttt tcgcagcatt atccgccgct ctctggggag
     3901 gtgcaacaca ggccgcagat gccgccgttg tcgcttcgct taaacccgtt gggttcatcg
     3961 cttctgccat tgctgatggt gtaacagaaa cggaggtttt actgcctgac ggcgcttcag
     4021 aacatgatta ttcactgcgt ccatcggatg taaaacgctt acagaacgcg gacttagtcg
     4081 tttgggttgg cccggagatg gaagcgttta tgcaaaaacc ggtaagcaaa ttaccagaag
     4141 cgaagcaggt aacgattgcg cagcttgaag atgtgaaacc gctactgatg aaaagtattc
     4201 acggcgatga tgatgatcac gaccacgcgg aaaaaagtga cgaagatcac catcacggcg
     4261 atttcaacat gcatctttgg ctttccccag agatagcgcg ggctacagcg gttgcaatcc
     4321 atggaaaatt agtggaactt atgccgcaaa gtcgagccaa acttgacgcc aacctgaagg
     4381 attttgaggc acaattggcc tcaaccgaaa cgcaggttgg taacgagctc gcgccgctca
     4441 aggggaaagg ttatttcgtt tttcacgatg cttacggcta tttcgaaaaa cagttcggac
     4501 tgacaccgct tggtcatttt accgttaacc ctgagattca acctggcgcg cagcgtttac
     4561 atgaaataag aacacagttg gttgagcaaa aagcaacctg cgtttttgct gagccacagt
     4621 tcaggccagc ggtcgttgag agcgtcgcac gagggacatc cgttcgtatg ggaacgttgg
     4681 atcccctggg gacgaatatc aaactgggta aaacaagcta ttcagaattc ctgagtcaat
     4741 tagccaacca gtatgcgagc tgcctgaaag gagattaatg aggaagtgat tacgtgcaac
     4801 agatagcccg ctctgtcgcc ctggcgttta ataatttacc gcgaccacac cgcgttatgt
     4861 tggggtcgct caccgttctt actctggccg tcgctgtctg gcggccctat gtttatcacc
     4921 gtgatgccac gccaattgtc aaaaccattg agctggaaca gaacgaaatt cgttcgctct
     4981 tacctgaagc cagtgagccg attgatcaag ctgcacaaga agatgaagcc attccccagg
     5041 acgaactgga tgacaaaatc gccggtgaag cgggcgtgca tgaatatgtt gtttccactg
     5101 gcgatacgct aagcagcatt ctcaatcagt atggtattga tatgggtgat atcacccaac
     5161 tcgctgctgc cgacaaagaa ttgcgtaacc tgaaaatcgg tcaacaactc tcctggacat
     5221 taaccgcgga cggcgaactg cagcgcctca cctgggaagt gtctcgtcgt gaaacccgaa
     5281 cctatgaccg tactgccgct aacggtttta aaatgaccag cgaaatgcag caaggagagt
     5341 gggttaacaa tctgctgaaa ggtaccgtcg gagggagctt tgttgccagc gccagaaacg
     5401 ccggtttaac cagcgccgaa gtgagcgcag tgattaaagc catgcagtgg caaatggatt
     5461 tccgcaaact gaaaaaaggc gatgaatttg cggtattaat gtctcgagaa atgcttgatg
     5521 gtaaacgtga gcaaagccag ctgctgggcg tacgtttgcg ttcagaaggt aaagattatt
     5581 acgcaatccg cgctgaggat ggcaaattct acgatcgtaa cggtactggt ctggcgaaag
     5641 gattcttgcg attcccgacg gcgaaacagt tccgtatctc gtctaacttt aacccgcgtc
     5701 gaactaatcc ggtgacaggt cgcgttgcac cacacagagg tgttgatttc gccatgccac
     5761 aaggtacgcc agtgctttca gtgggtgacg gtgaagtggt ggttgccaaa cgcagtggcg
     5821 cagcaggtta ttatgtggct attcgtcatg gtcgcagcta caccacgcgt tatatgcact
     5881 tgcgcaagat tctggtgaaa ccgggacaga aggtgaaacg tggcgaccgt atcgcgcttt
     5941 ccggtaatac cggacgttca actgggccgc atctgcacta tgaagtatgg ataaaccagc
     6001 aggccgtaaa cccgctgacg gcaaaactgc cgcgtaccga agggctgacc ggctccgatc
     6061 gtcgcgaatt cctggcgcag gccaaagaga ttgtgccgca gctacggttt gattaattaa
     6121 catccattcg cagccggtac gcagtcagta ccggcttttt ttatttggtg cggggcaagt
     6181 tgcgccgcta cactatcacc agattgattt ttgccttatc cgaaactgga aaagcatgga
     6241 aacgaaaaaa aataatagcg aatacattcc tgagtttgat aaatcctttc gccacccgcg
     6301 ctactgggga gcatggctgg gtgtagcagc gatggcgggt atcgctttaa cgccgccaaa
     6361 gttccgtgat cccattctgg cacggctggg acgttttgcc ggacgactgg gaaaaagctc
     6421 acgccgtcgt gcgttaatca atctgtcgct ctgctttcca gaacgtagtg aagctgaacg
     6481 cgaagcgatt gttgatgaga tgtttgccac cgcgccgcaa gcgatggcaa tgatggctga
     6541 gttggcaata cgcgggccgg agaaaattca gccgcgcgtt ggctggcaag ggctggagat
     6601 catcgaagag atgcggcgta ataacgagaa agttatcttt ctggtgccgc acggttgggc
     6661 cgtcgatatt cctgccatgc tgatggcctc gcaagggcag aaaatggcag cgatgttcca
     6721 taatcagggc aacccggttt ttgattatgt ctggaacacg gtgcgtcgtc gctttggcgg
     6781 tcgtctgcat gcgagaaatg acggtattaa accattcatc cagtcggtac gtcaggggta
     6841 ctggggatat tatttacccg atcaggatca tggcccagag cacagcgaat ttgtggattt
     6901 ctttgccacc tataaagcga cgttgcccgc gattggtcgt ttgatgaaag tatgccgtgc
     6961 gcgcgttgta ccgctgtttc cgatttatga tggcaagacg catcgtctga cgattcaggt
     7021 gcgcccaccg atggatgatc tgttagaggc ggatgatcat acgattgagc ggcggatgaa
     7081 tgaagaagtc gagatttttg ttggtccgcg accagaacaa tacacctgga tactaaaatt
     7141 gctgaaaact cgcaaaccgg gcgaaatcca gccgtataag cgcaaagatc tttatcccat
     7201 caaataaaaa agcctctcgc gaggagaggc cttcgcctga tgataagttc aagtttgctt
     7261 cagaatattc gaaatctgtt gaactatcat tgaactatag gccggatgtg gcgttttcgc
     7321 cgcatccggc aacgtactta ctctaccgtt aaaatacgcg tggtattagt agaacccacg
     7381 gtactcatca cgtcgccctg ggtgacaatc accaggtcac cagacatcaa gtaaccttta
     7441 tcgcgcagca gattaaccgc ttcgctggca gctgctacgc cgtcattagc gctatcaaag
     7501 tgcaccggcg taacgccacg atagagagca gtcaggttca gcgtacgttc atggcgcgac
     7561 atggcgaaaa ttggtagacc agagctgata cgggaggtca tcagcgcggt acgacccgat
     7621 tcggtcatgg taatgatcgc cgtaacgccc ttcaggtggt tagctgcgta cattgccgac
     7681 atggcaatag cttcttccac attgtcgaac tgaacgtcca gacggtgttt agaaacgttg
     7741 atgctcggga ttttttccgc acccaggcaa acgcgcgcca tggctgcaac agtttctgac
     7801 ggatactgcc cagcggcagt ttctgcagac agcatcacag cgtcagtacc atccagaacg
     7861 gcgtttgcta cgtccatgac ttctgcacgc gtcggcatcg ggttagtaat cattgactcc
     7921 atcatctggg tcgccgtgat taccgctcgg tttagctgac gcgcacgacg gatcaacgct
     7981 ttctgaatgc cgaccagttc cgggtcgcca atttccacac cgaggtcgcc acgtgcaacc
     8041 attaccacgt cagaggcgag gatgatgtca tccattgcat cctggctgca aacggcttcc
     8101 gcacgttcaa ccttggcaac aattttcgca tcacatcctg catcgcgtgc caggcgacgg
     8161 gcatagttca gatcttcgcc acagcgtggg aaggagacag ccaggtaatc tacgccaatc
     8221 aacgccgcag tcttaatgtc tgctttgtct ttttcggtca gcgcttcagc cgacaaaccg
     8281 ccgccaagtt tgttgatacc tttattgttg gagaggggac caccgacggt gacttcggtg
     8341 aacactttca tgccctgaac ttccagtact tttaactgga cgcgaccatc gtccagcagc
     8401 aggatgtcac caggcacgac gtcagcaggc aggcctttgt agtcgatacc gactttttct
     8461 ttgtcgcctt cacctttacc caggttggcg tcgagcagga atttatcccc aatattgagg
     8521 aaaactttgc cttctttaaa ggtggataca cggattttgg gcccctggag gtcacccaga
     8581 atagccacat gacgccccag ttttgcggca atctcacgaa ctttatccgc gtgcattttg
     8641 tgatcttcag gcgagccgtg agaaaagttc atacgtacaa cgttggcacc cgccgcgata
     8701 actttttcaa gattattatc gcgatctgtt gctgggccta acgtggtaac gatttttgtt
     8761 ctgcgaagcc ttttggacat gtaatactcc gttgactgaa acaaccaggt gttgcttgaa
     8821 catgaaatcc gaatgaaata acgccgcgat ggcgataaat taatctcatt cggatgtcgt
     8881 ataagattag gacagtgaca gtcgctttta gcgatcgtca cttaaattaa gtaactgctt
     8941 atcaaaacgc gattccttca gcgcttcctt gacccgcttc aagttatctc tgaattttgc
     9001 accgcgtcgc aaagtaaatc ctgtcgccag cacatctatc acggtcagct gtgcaagtcg
     9061 agaaaccatg ggcatataaa tgtcagtatc ttccggtacg tcgagggtaa ttgccagcgt
     9121 tgcttcccgg gcgagtgggg tacccgcaga ggtgagggca atcaccatgg cgtcgttttc
     9181 gcgtgccagc tgcgccagct cgaccagatt ttttgttctt cctgtgtgag aaatcagcac
     9241 caccacgtct ccgtcgctac aattcataca actcatgcgt tgcagcacga tatcatcgga
     9301 gtacaccacc ggaacattaa aacgaaagaa cttattcatc gcatcgtggg caacggcggc
     9361 agaagagcct aatccgaaaa aggcgatttt ttttgcctga gtgagcaagt cgacggcgcg
     9421 gttgatggca gatttatcca gtgaatgacg gacatgatcg agcgttgcca ttgcggactc
     9481 aaatattttc cccgtgtatg attcaacgct gtcatcttca ttgacgttgc gattaacata
     9541 gggagtgcca ttcgccagac tttgtgccag atgaagttta aaatcaggaa aaccgcgcgt
     9601 gtccatgctg cgacagaaac gattcaccgt cggttcgcta acattggctt ccagcgccag
     9661 agcagcaata ctcgaatgga tcgcgttatc gggcgaagcc agaatgacct cggcaacttt
     9721 gcgctctgat ttgctcaaat gttccagctg agactggatt ttttccagca tattcatgat
     9781 gtaaagagac tcacgggtaa tgacgatttc cgcactgaaa gaaatcgaaa tgcagttttg
     9841 tcagatatta cgcctgtgtg ccgtgttaat gacaaaagca gataaaaaag ttgttatttt
     9901 ttttcataac atgatcagtg tcagattttt accgaatgga aaacgatgat ttttttatca
     9961 gttttgccgc actttgcgcg cttttcccgt aatcgcacgg gtggataagc gtttacagtt
    10021 ttcgcaagct cggaaaagca gtacagtgca ccgtaagaaa attacaagta taccctggct
    10081 taagtaccgg gttagttaac ttaaggagaa tgacatggcg gtaacgcaaa cagcccaggc
    10141 ctgtgacctg gtcattttcg gcgcgaaagg cgaccttgcg cgtcgtaaat tgctgccttc
    10201 cctgtatcaa ctggaaaaag ccggtcagct caacccggac acccggatta tcggcgtagg
    10261 gcgtgctgac tgggataaag cggcatatac caaagttgtc cgcgaggcgc tcgaaacttt
    10321 catgaaagaa accattgatg aaggtttatg ggacaccctg agtgcacgtc tggatttttg
    10381 taatctcgat gtcaatgaca ctgctgcatt caaccgtctc ggcgcgatgc tggatcaaaa
    10441 aaatcgtatc actattaact actttgccat gccgcccagc acttttggcg caatttgcaa
    10501 agggcttggc gaggcaaaac tgaatgctaa accggcacgc gtagtcatgg agaaaccgct
    10561 ggggacgtcg ctggcgacct cgcaggaaat caatgatcag gttggcgaat acttcgagga
    10621 gtgccaggtt taccgtatcg accactatct tggtaaagaa acggtgctga acctgttggc
    10681 gctgcgtttt gctaactccc tgtttgtgaa taactgggac aatcgcacca ttgatcatgt
    10741 tgagattacc gtggcagaag aagtggggat cgaagggcgc tggggctatt ttgataaagc
    10801 cggtcagatg cgtgacatga ttcagaacca cctgctacaa attctctgca tgattgcgat
    10861 gtctccgcca tctgacctga gcgcagacag catccgcgat gaaaaagtga aagtactgaa
    10921 gtccctgcgc cgcatcgacc gctccaacgt acgcgaaaaa accgtacgcg ggcaatatac
    10981 tgcgggcttc gcccagggca aaaaagtgcc gggatatctg gaagaagagg gcgcgaacaa
    11041 aagcagcaat acagaaacct tcgtggcgat ccgcgtcgac attgataact ggcgctgggc
    11101 cggtgtgcca ttctacctgc gtactggtaa acgtctgccg accaaatgtt ctgaagtcgt
    11161 ggtctatttc aaaacacctg aactgaatct gtttaaagag tcgtggcagg atctgccgca
    11221 gaataaactg actatccgtc tgcaacctga tgaaggcgtg gatatccagg tactgaataa
    11281 agttcctggc cttgaccaca aacataacct gcaaatcacc aagctggatc tgagctattc
    11341 agaaaccttt aatcagacgc atctggcgga tgcctatgaa cgtttgctgc tggaaaccat
    11401 gcgtggtatt caggcactgt ttgtacgtcg cgacgaagtg gaagaagcct ggaaatgggt
    11461 agactccatt actgaggcgt gggcgatgga caatgatgcg ccgaaaccgt atcaggccgg
    11521 aacctgggga cccgttgcct cggtggcgat gattacccgt gatggtcgtt cctggaatga
    11581 gtttgagtaa tatcagcgct tatcctttat ggttatttta ccggtaacat gatcttgcac
    11641 agattgtaga acaattttta cactttcagg cctcgtgcgg attcacccac gaggcttttt
    11701 ttattacact gactgaaacg tttttgccct atgagctccg gttacaggcg tttcactcat
    11761 aaatcctctg aatgaaacgc gttgtgaatc atcctgctct gacaactcaa tttcaggagc
    11821 ctttatgaat ccacaattgt tacgcgtaac aaatcgaatc attgaacgtt cgcgcgagac
    11881 tcgctctgct tatctcgccc ggatagaaca agcgaaaacg tcgaccgttc atcgttcgca
    11941 gttggcatgc ggtaacctgg cacacggttt cgctgcctgc cagccagaag acaaagcctc
    12001 tttgaaaagc atgttgcgta acaatatcgc catcatcacc tcctataacg acatgctctc
    12061 cgcgcaccag ccttatgaac actatccaga aatcattcgt aaagccctgc atgaagcgaa
    12121 tgcggttggt caggttgcgg gcggtgttcc ggcgatgtgt gatggtgtca cccaggggca
    12181 ggatggaatg gaattgtcgc tgctaagccg cgaagtgata gcgatgtctg cggcggtggg
    12241 gctgtcccat aacatgtttg atggtgctct gttcctcggt gtgtgcgaca agattgtccc
    12301 gggtctgacg atggcagccc tgtcgtttgg tcatttgcct gcggtgtttg tgccgtctgg
    12361 accgatggca agcggtttgc caaataaaga aaaagtgcgt attcgccagc tttatgccga
    12421 aggtaaagtg gaccgcatgg ccttactgga gtcagaagcc gcgtcttacc atgcgccggg
    12481 aacatgtact ttctacggta ctgccaacac caaccagatg gtggtggaat ttatggggat
    12541 gcaattgcca ggctcttctt ttgttcatcc ggattctccg ctgcgcgatg ctttgaccgc
    12601 tgccgctgcg cgtcaggtta cacgcatgac cggtaatggt aatgaatgga tgccgatcgg
    12661 taagatgatc gatgagaaag tggtggtgaa cggtatcgtt gcactgttgg cgaccggtgg
    12721 ttccactaac cacaccatgc acctggtggc gatggcgcgc gcggccggta ttcagattaa
    12781 ctgggatgac ttctctgacc tttctgatgt tgtaccgctg atggcacgtc tctacccgaa
    12841 cggtccggcc gatattaacc acttccaggc ggcaggtggc gtaccggttc tggtgcgtga
    12901 actgctcaaa gcaggcctgc tgcatgaaga tgtcaatacg gtggcaggct ttggtctgtt
    12961 tcgttatacc cttgaaccat ggctgaataa tggtgaactg gactggcggg aaggggcgga
    13021 aaaatcactc gacagcaatg tgatcgcttc cttcgaacaa cctttctctc atcatggtgg
    13081 gacaaaagtg ttaagcggta acctgggccg tgcggttatg aaaacctctg ccgtgccggt
    13141 tgagaaccag gtgattgaag cgccagcggt tgtttttgaa agccagcatg acgttatgcc
    13201 ggcctttgaa gcgggtttgc tggaccgcga ttgtgtcgtt gttgtccgtc atcaggggcc
    13261 aaaagcgaac ggaatgccag aattacataa actcatgccg ccacttggtg tattattgga
    13321 ccggtgtttc aaaattgcgt tagttaccga tggacgactc tccggcgctt caggtaaagt
    13381 gccgtcagct atccacgtaa caccagaagc ctacgatggc gggctgctgg caaaagtgcg
    13441 cgacggggac atcattcgtg tgaatggaca gacaggcgaa ctgacactgc tggtagacga
    13501 agcggaactg gctgctcgcg aaccgcacat tcctgacctg agcgcgtcac gcgtgagaac
    13561 aggacgtgaa ttattcagcg ccttgcgtga aaaactgtcc ggtgccgaac agggcgcaac
    13621 ctgtatcact ttttaagacg acaaatttgt aatcaggcga gagaaaactc tgatgaaaaa
    13681 ctggaaaaca agtgcagaat caatcctgac caccggcccg gttgtaccgg ttatcgtggt
    13741 aaaaaaactg gaacacgcgg tgccgatggc aaaagcgttg gttgctggtg gggtgcgcgt
    13801 tctggaagtg actctgcgta ccgagtgtgc agttgacgct atccgtgcta tcgccaaaga
    13861 agtgcctgaa gcgattgtgg gtgccggtac ggtgctgaat ccacagcagc tggcagaagt
    13921 cactgaagcg ggtgcacagt tcgcaattag cccgggtctg accgagccgc tgctgaaagc
    13981 tgctaccgaa gggactattc ctctgattcc ggggatcagc actgtttccg aactgatgct
    14041 gggtatggac tacggtttga aagagttcaa attcttcccg gctgaagcta acggcggcgt
    14101 gaaagccctg caggcgatcg cgggtccgtt ctcccaggtc cgtttctgcc cgacgggtgg
    14161 tatttctccg gctaactacc gtgactacct ggcactgaaa agcgtgctgt gcatcggtgg
    14221 ttcctggctg gttccggcag atgcgctgga agcgggcgat tacgaccgca ttactaagct
    14281 ggcgcgtgaa gctgtagaag gcgctaagct gtaaaagtca aaatgcccga tctaggatcg
    14341 ggcatttttg tagcgttttt tgccgggttt aaccctgtac ttttacctgt ccggcggcgt
    14401 gcttcgcgcg ttcaatggcg tcaacaacac tctctgaagt agccagtacc acccccagac
    14461 gacggctgcc atcaatttcc ggcttaccaa ataaacgaat ctgcaaatct gcgcctacgg
    14521 cattctgcac attatcaaac gtgacattct gactggtcag ttgtggcaga ataacggcag
    14581 aagctgcagg accatactga cggatcccgc caacaggaag tccgaggaag gcacgtacat
    14641 gcagggcaaa ctctgagaga tcttgagaaa ttaacgtcac catcccggta tcatgtggac
    14701 gaggggagac ctcactgaaa atcacctcat caccacagac aaacagctcg acaccaaaca
    14761 gaccataacc gcccagtgcc agcaccactt tacgggcaat ctcctgcgca cgttcaaggg
    14821 caagcgggct catttgctgt ggttgccagg attcacggta gtcgccatct tcctggcgat
    14881 gacctactgg tgcacagaaa tggacgccat ccaccgcgct gacggttagc agggtaattt
    14941 cgaagtcaaa cttaacgacg ccttcaacaa ttacgcggcc cgctccggcg cgaccgcctt
    15001 gctgagcgta ttcccatgcc tgagcaagtt gctctgcaga gcgaataaac gtctgcccct
    15061 tgccggaaga gctcatcacc ggttttacaa tgcagggata gccaatggcg gcaaccgcct
    15121 cgcggaaaag gcttttgcta tcggcaaaac gataagtgga agtgggcagc tgcagctctt
    15181 ctgccgccag gcgacggata ccctcgcgat tcatcgttaa tttcgttgcg cgagcgcagg
    15241 ggacaacatt cagtccttcc tcttcaagtt ggatcagcat atcggtggca atagcttcga
    15301 tctccggcac gatataatgt ggtttttcca gttcaaccac acggcgtaat gcatcaccat
    15361 caagcatatt aatgacatgg gagcgatgcg cgacatgcat ggctggtgcg tcggcatagc
    15421 gatcgacggc aatcacctct acgccgagac gctgacactc gattgccact tctttaccca
    15481 gttcaccgga gcctaataac atcacgcgag ttgctgccgg acgcagcgca gtgcctaata
    15541 acgtcataac gaatatcccc tgattaattc cgcgcgcagt ataaacgaaa acgtttgcgt
    15601 gtgtctttat tgctgatgtt gatttctcaa ccgaaaagaa atatactgta taaaatcaca
    15661 gttattatga gaggctgcaa atggcggttg aagttaaata cgtagtcatt cgtgagggcg
    15721 aagagaaaat gtcgtttacc agcaaaaagg aagccgatgc ttatgacaaa atgcttgata
    15781 cggcggatct gcttgacacc tagctgacaa attctccagt gcaaatggaa gacgagcaac
    15841 gtgaagccct ttcgctatgg ctggcagaac aaaaagatgt gctgagcacc attctgaaaa
    15901 ccggcaaatt accgtcccca ctggtcgtcg gtgctgaatc cgaagaggaa gatgcctcac
    15961 acgcggcctg aagcaacttg cgctcctgac aatttgtacc atgctttctc ctgaatcatt
    16021 gtgctatgga gaaaaacatg aaaaaaagag gggcgttttt agggcggttg ttggtttctg
    16081 cctgcgcatc agttttcgct gccaataatg aaaccagcaa gtcggtcact ttcccaaagt
    16141 gtgaaggtct ggatgctacc ggaattgccg cgagcgtaaa acgtgattat caacaaaatc
    16201 gcgtggcgcg ctgggcagat gatcaaaaaa ttgtcggtca ggccgatccc gtggcctggg
    16261 tcagtttgca ggacattcag ggtaaagatg ataaatggtc agtaccgcta accgtgcgtg
    16321 gtaaaagtgc cgatattcat taccaggtca gcgtggactg caaagcggga atggcggaat
    16381 atcagcggcg ttaatggctt ctgataattc gattgaccgt tggcggatgc ggcgcgagcg
    16441 ccttgtccgt cccagaacgc accacaatcg taggcctgag aagacgcaac agcgtcgcat
    16501 cgggcaatca gcatcaaaca atgccactca aaacgacaaa ctactttcac ctaaaatacc
    16561 cttatcacct gatatacagt aaatacctta cctggcgcga atttctttga ataacggcct
    16621 accgccccgc ttatttgcgc atcttttgtc tcgatataca ttttctgaca tcctcatccg
    16681 ctatgctaca ggcactaaaa atggttattg aggagcaaat atggctaact ggttaaatca
    16741 actgcaatcc ctgcttgggc aaagcagttc ttctacctcc tcgtctgcgg atcagggatt
    16801 gggcaaactg ttagtgccag gcgcattagg cggactggct gggctgctgg tcgcaaataa
    16861 atcagcacgt aaacttctta cgaaatatgg caccaacgcg ttactggttg gcggcggagc
    16921 ggtagcgggt acggtgctgt ggaataaata caaagataaa attcgcgcgg cgcatcagga
    16981 cgaaccgcag tttggcgcgc aaagtacgcc gctggatgag cgtacggaac gtttgatcct
    17041 tgcgctggtc tttgccgcta aaagtgatgg tcatattgat gccaatgaac gtgcggcaat
    17101 cgaccagcaa ttgcgtgaag ccggcgtgga agagcagggg cgtgtactca ttgagcaggc
    17161 aatcgaacaa ccgctggatc cacaacgcct ggctaccggg gtccgcaatg aagaagaggc
    17221 gctggaaatc tatttcctga gctgcgcggc tattgatatt gaccatttta tggaacgcag
    17281 ttacctgaat gcactgggtg acgcgctgaa aatcccccag gatgttcgtg aaggcattga
    17341 acgcgatctc gaacagcaaa aacgcacttt ggcggaataa ttccgctctc tggatagcct
    17401 gaagttgact atttcgggct atccagaacc gcacagccat ttgaacaggc tgattttctc
    17461 tgtctgcata acattcgata tccgcgccat tacctgtcat catctaagca atgacgaccc
    17521 tgtttcgctt gcatccccgg tgagttttgc cacccttata agatgtttca accagaaaga
    17581 acaataacat gctaccaaaa gccgcccgca ttccccacgc catgacgctt catggcgata
    17641 cgcgcatcga taattactac tggctgcggg acgatacgcg ttctcagcca gaagtcctgg
    17701 actacctgca acaagaaaat agttacggtc atcgggtgat ggcctcacaa caagccttgc
    17761 aggatcgcat cttaaaggaa atcatcgacc gcattccgca aagagaagtt tctgcgccct
    17821 acataaaaaa tggctaccgc tatcggcata tttatgaacc aggctgtgaa tatgctatct
    17881 accagcgtca atcggcattc agcgaagagt gggatgagtg ggaaacattg ctcgatgcca
    17941 acaagcgcgc agctcatagt gagttttatt cgatgggcgg aatggcgatt acgcccgata
    18001 acaccattat ggcgctggca gaagattttc tttcccgacg ccagtacggc attcgttttc
    18061 gtaatctgga aacaggtaac tggtacccgg aactgctgga taacgttgaa cccagctttg
    18121 tctgggcaaa tgactcctgg actttctact atgttcgcaa gcatccggtg acgctgctgc
    18181 cttatcaggt ctggcgtcac gccatcggta cgccagcatc gcaagataca ctgatctacg
    18241 aagaaaaaga cgatacctat tatgtcagcc tgcataaaac gacgtcgaag cactatgtcg
    18301 tcattcatct ggccagcgcc accaccagtg aagttcgcct gctggacgcg gaaatggccg
    18361 atgccgagcc gtttgttttt ctgccgcgcc gcaaagatca cgaatacagc cttgatcact
    18421 accagcatcg tttttatctg cgttccaatc gccacggcaa aaactttggc ttataccgta
    18481 cccgtatgcg tgatgagcaa cagtgggaag agttaattcc gccacgtgaa aacatcatgc
    18541 tggaagggtt tacgctgttt accgactggc tggtggttga agagcgtcag cgcgggttaa
    18601 ccagtttgcg ccaaattaac cgcaagaccc gggaagtcat tggcattgcc tttgatgatc
    18661 cggcctatgt gacctggatt gcctacaatc cagaacctga aaccgcgcga ttgcgttatg
    18721 gttattcttc catgaccaca ccagacactt tgtttgaact ggatatggat accggtgagc
    18781 gtcgtgtatt aaaacaaaca gaagtgcccg gttttgatgc ggcgaattac cgcagtgaac
    18841 acctgtggat agtcgcccgt gatggcgtcg aagttccggt ttcgctggtc tatcatcgca
    18901 aacattttcg caaaggacac aacccgttgc tggtgtatgg ctatggttct tacggcgcaa
    18961 gtattgatgc cgatttcagt tttagccgct tgagtttgtt agatcgtggc tttgtctacg
    19021 ccattgtcca tgttcgcggc ggtggtgagc tggggcaaca atggtacgaa gacggtaaat
    19081 ttctgaagaa gaaaaatacg tttaatgatt atcttgatgc ctgcgatgca ttgttaaaac
    19141 tgggctatgg ctctccttcg ctctgttatg cgatgggcgg aagtgcgggg ggcatgttga
    19201 tgggcgttgc aattaatcaa cgcccggaat tattccacgg cgttatcgcc caggtaccgt
    19261 ttgttgatgt tgtaacaacg atgcttgatg aatcaattcc tcttaccact ggtgagtttg
    19321 aagagtgggg aaatccgcag gatccgcaat attacgagta catgaaaagc tacagcccgt
    19381 atgacaacgt caccgcacag gcttatccgc atttactggt aacgaccggt ttacacgatt
    19441 ctcaggtgca acattgggaa ccggcaaaat gggtcgctaa attgcgcgag ctgaaaaccg
    19501 atgaccatct tttattgctc tgtaccgaca tggactcagg ccatggcggt aaatctggcc
    19561 gctttaaatc gtacgaaggc gtagcgatgg aatatgcttt tctggtcgcg ctggcgcagg
    19621 gaacattacc cactacgcct gcggattaag tattttccag ataatgtttc agtgttaaac
    19681 gcagctccgg gctcatgctg tccaggttat taaataacca gcgcagatag cccggatcgc
    19741 gttcggcaac gtcggaaacc gctttgccac ggtatttgcc aaaggtgaag gtcgtcatca
    19801 acgacggacg tccggtgata tcggccatct gttctgccgt ccagccggag gtgttcataa
    19861 tatcgataag caacgcggcg gtgatataac agtcatacaa cgcgcggtga tgatgcaggc
    19921 ccggcggtgt ctgtacattg agcttgcgtg ttttatataa cgccatattg ctgtacttga
    19981 gccctggcca caaacgacgg gccagtttca tagtgcaaat ccactcaccg ggcatctcag
    20041 gcagtacgcg gcggtcaaag ctggcgttgt gcgcgacata ccattcacta ccgtaatagt
    20101 gtgggatcac atcttcaatc cacggtttat cggcgaccat ggcttcggtg atgcgatgaa
    20161 tcgccatcgc ttgtggacta ataggacgat cggggcgcac caggaggctc atggggttga
    20221 cgatttttcc gtcaatgaca tcaacagagg caatctcaac gatccctccc tgcaaaccgc
    20281 aggtttctgt atcgataatg cgcaacatgg catgctccag gccgaaaaag cctagcgtaa
    20341 tggaatgata tcgccttgcc aaccctgtga tgaacgctga ccgactaaca ataatgaacc
    20401 acgatcggcg cgaacgatga gacgaccata ttcatcccat agtgcactgt taccacgggc
    20461 atttgccatc agcacggcaa tcgagtactg atgtgaaaaa aattgtaagc gacgggccga
    20521 cgccagcagg tcgggttctc ccagacattg ccctgtcgta aacagtgaac aggtcgggtc
    20581 catatccata ccttgcggtt gttcatcgac caccgtgatg gttctggaac gacggcccag
    20641 acatgcacca tgactctgat ggtagttccc aggcgttttt cgccacggtg cgaacaccgc
    20701 aattccacga ataaagcgat cgttatattc aacgggaagg ccggcaatga tggtcattcg
    20761 ccaggtcgtt gcggcataac aaagcgggtc taacagtgaa agatcgggtg gggcaggaag
    20821 ggcacgtcgc gaataatcac accccagtaa agaaagcgat ggaaaaacta acagctgaca
    20881 ttgttgcctg gcggcggccc tcacaaactc cagatgatga gctacctgtt cggtgagtga
    20941 ggttttacga ggctcatatt gcgctgctgc aactttccag aacgacataa tgacttcctt
    21001 ttcaaaatga ctgcaaatta tgggggcggt ttagaaaata ctcataattt agcaggcgtt
    21061 atgtaagaaa gtgtaactct gtataagttt tatttaagtt tgggctcgta aggtaaacgt
    21121 gacagattga ccgaagccaa acggtgggca ataagccgct cgcgaaacca gctgcgcaaa
    21181 tgttctggct gttcacgttc aaccgcttca gcgatcaccg gcatattgta gcgttcttta
    21241 aaagccaccc cggccgccgc caaatcgaca ttcactttat ccatttctgt ttgatccagt
    21301 ttagccagat tcttcagcat tcttaatctc ctcatcattc gcgggcaaaa gttactaaga
    21361 gtgagcctgg atggcaagcc ggaattgatg atcctcgact cagacattag acaaggttat
    21421 gctctgtaaa aaagttatta caaaaaggaa tagataatat ggcttcaact gcacgctccc
    21481 ttcgctacgc gctgacaatc ctgaccactt cgttggtgac accttccgtc tgggcacatg
    21541 cgcatttaac gcatcagtat cctgcggcaa acgcgcaagt gacagctgcc ccgcaggcaa
    21601 tcactttaaa cttctcggaa ggcgttgaaa caggattcag tggtgcaaaa atcacggggc
    21661 caaaaaacga aaatattaaa acattaccag cgaagcggaa tgagcaggac caaaagcaac
    21721 tgattgttcc actggccgat tcgctgaaac ccggaaccta caccgttgac tggcatgttg
    21781 tttcggtgga cgggcataaa acgaaaggac actacacctt tagcgtgaaa taaatcatgc
    21841 tggcgtttac ctggatcgcg ctgcgattta tccatttcac ctcgttgatg ctggtctttg
    21901 gctttgcaat gtatggtgcc tggctggccc ccttaatgat tcgtcgcttg ctgacgaagc
    21961 gttctctgcg tttacaacag cacgctgccg tctggagttt gatcagtgcc actgcaatgc
    22021 tcgccgttca gggcgggttg atgggaacgg gatggacaga tgtattttcg ccaaacattt
    22081 ggcaggctgt tttgcaaacg cagtttggtg gcgtctggtt atggcaaatt gttctcgccc
    22141 tcgtaacgtt gatcgtcgcc cttatgcaac cgcggaatat gccacgtttg ttgtttatgc
    22201 tcactaccgc gcaatttatt ctgctggcgg gagtggggca tgcgacgctg aatgaagggg
    22261 taacagcgaa aatccatcag actaatcacg cgattcacct gatttgtgcg gcggcgtggt
    22321 ttgggggatt actgccggtg ctctggtgta tgcaactcat caaaggccgt tggcgacacc
    22381 aggctattca ggcgctgatg cgtttttcct ggtgcgggca ttttgcggtg atcggcgtac
    22441 tggcaagcgg cgtgcttaat gcattgttga ttaccggatt tccccccacg cttacgactt
    22501 actagggcca actcttattg ctcaaagcca tcctggtgat gattatggtg gtcattgcgc
    22561 tggctaatcg ttatgttctt gtaccgcgca tgaggcagga tgaagatcgt gcggcaccct
    22621 ggtttgtgtg gatgactaaa ctcgaatggg cgataggtgc ggtagtgctg gtgatcatca
    22681 gcctgcttgc gactctcgaa cctttttgat ggattacgaa aatgatgaaa aaaagtattc
    22741 tggcgtttct gttactcacc agttctgcag cggcgctggc ggctccacag gtgataaccg
    22801 tgagtcgttt tgaagtgggt aaagacaaat gggcgtttaa tcgcgaagag gtaatgctga
    22861 cttgccgacc gggtaatgct ttgtatgtca tcaacccaag taccctcgtg cagtatcctt
    22921 taaacgatat cgcacaaaag gaagttgcca gtgggaagac caatgcccaa cccatttcgg
    22981 tgattcagat tgatgatcct aacaatcccg gcgaaaaaat gagtctggca ccgtttatag
    23041 aacgagctga aaaactctgt taattaccta aaatagcctt ttgatttcca ataaaaaaaa
    23101 cgcctcagtt ctttcaccag aacgggcggt ttttaacatt taagctgatg accaccgcgc
    23161 tttttattga ccattttgca cgcaaactgg aaaacctggc gtcgtcatct attcttaaag
    23221 ggcaaggcaa ctaagcctgc attaatgcca acttttagcg cacggctctc tcccaagagc
    23281 catttccctg gaccgaatac aggaatcgta ttcggtcttt ttttaattgt atttaaaatc
    23341 aattagttgc aaacgtctcc ccgaaattcc ccgaaattta ctcgaatttc tgtattccgg
    23401 tcttttttgg ttatatcaca accaaaatac atttaacaat ccatttacgt taaaatcaga
    23461 gcagtaagta cgttttttct ctctcatcaa gatacatttt tgttgtcttc tccgatgtgt
    23521 ggccaagtag acgctgagca aattcttctc cacatatttc tttgtacaat cgtccagcca
    23581 ggcttctgat ttcgtgaaaa gttggtgggt tttcactgaa caggatacct gttaattttc
    23641 tggctgcgac aaatttcttt gtcaggccgt ccgggtgaat gctgccgtca gggctgtttt
    23701 ttctaatccc ggcactgatt agataatccc ccctacttac catgcggcac tgttcaacta
    23761 ctgcaccaag tcgtaaacca gcgacaggaa ggctgagtga cagggggata gcaatcatca
    23821 ttcctgtctt aatttgcctg atgtggagac gatcatcata aatatcactg aaccgcatat
    23881 tcgttatgtc ttcgcgacgt tgtcctgtta caagggctaa gtccatagct aatgggaa
//
LOCUS       DAJTYG010000057        23683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTYG010000057 DAJTYG010000000
VERSION     DAJTYG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.057"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            570..2498
                     /gene="thrS"
                     /locus_tag="OO568_002433"
     CDS             570..2498
                     /gene="thrS"
                     /locus_tag="OO568_002433"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6641755.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKIPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2502..3044
                     /gene="infC"
                     /locus_tag="OO568_002434"
     CDS             2502..3044
                     /gene="infC"
                     /locus_tag="OO568_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6641756.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3141..3338
                     /gene="rpmI"
                     /locus_tag="OO568_002435"
     CDS             3141..3338
                     /gene="rpmI"
                     /locus_tag="OO568_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6641757.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3391..3747
                     /gene="rplT"
                     /locus_tag="OO568_002436"
     CDS             3391..3747
                     /gene="rplT"
                     /locus_tag="OO568_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6641758.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3864..3988
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3870..3914
                     /gene="pheM"
                     /locus_tag="OO568_002437"
     CDS             3870..3914
                     /gene="pheM"
                     /locus_tag="OO568_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6641759.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4197..5180
                     /gene="pheS"
                     /locus_tag="OO568_002438"
     CDS             4197..5180
                     /gene="pheS"
                     /locus_tag="OO568_002438"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6641760.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5195..7582
                     /gene="pheT"
                     /locus_tag="OO568_002439"
     CDS             5195..7582
                     /gene="pheT"
                     /locus_tag="OO568_002439"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6641761.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEATEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7587..7886
                     /gene="ihfA"
                     /locus_tag="OO568_002440"
     CDS             7587..7886
                     /gene="ihfA"
                     /locus_tag="OO568_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6641762.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7987..8967
                     /gene="btuC"
                     /locus_tag="OO568_002441"
     CDS             7987..8967
                     /gene="btuC"
                     /locus_tag="OO568_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6641763.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9030..9581
                     /gene="btuE"
                     /locus_tag="OO568_002442"
     CDS             9030..9581
                     /gene="btuE"
                     /locus_tag="OO568_002442"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6641764.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYQDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9581..10330
                     /gene="btuD"
                     /locus_tag="OO568_002443"
     CDS             9581..10330
                     /gene="btuD"
                     /locus_tag="OO568_002443"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6641765.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10408..10872
                     /locus_tag="OO568_002444"
     CDS             10408..10872
                     /locus_tag="OO568_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6641766.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11119..11821
                     /gene="ydiV"
                     /locus_tag="OO568_002445"
                     /pseudo
     CDS             11119..11821
                     /gene="ydiV"
                     /locus_tag="OO568_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            11885..13321
                     /locus_tag="OO568_002446"
     CDS             11885..13321
                     /locus_tag="OO568_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6641767.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMPMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13325..13516)
                     /gene="hemP"
                     /locus_tag="OO568_002447"
     CDS             complement(13325..13516)
                     /gene="hemP"
                     /locus_tag="OO568_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6641768.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13648..14694)
                     /gene="aroH"
                     /locus_tag="OO568_002448"
     CDS             complement(13648..14694)
                     /gene="aroH"
                     /locus_tag="OO568_002448"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6641769.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14851..15684)
                     /gene="ppsR"
                     /locus_tag="OO568_002449"
     CDS             complement(14851..15684)
                     /gene="ppsR"
                     /locus_tag="OO568_002449"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6641770.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16016..18394
                     /gene="ppsA"
                     /locus_tag="OO568_002450"
     CDS             16016..18394
                     /gene="ppsA"
                     /locus_tag="OO568_002450"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6641771.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18451..20097)
                     /gene="fadK"
                     /locus_tag="OO568_002451"
                     /pseudo
     CDS             complement(18451..20097)
                     /gene="fadK"
                     /locus_tag="OO568_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20154..20447)
                     /locus_tag="OO568_002452"
     CDS             complement(20154..20447)
                     /locus_tag="OO568_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6641772.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20444..21733)
                     /locus_tag="OO568_002453"
     CDS             complement(20444..21733)
                     /locus_tag="OO568_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6641773.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21759..22727)
                     /locus_tag="OO568_002454"
     CDS             complement(21759..22727)
                     /locus_tag="OO568_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR6641774.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSRQFPFQPHLPL"
     gene            complement(22747..23511)
                     /locus_tag="OO568_002455"
     CDS             complement(22747..23511)
                     /locus_tag="OO568_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR6641775.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5840 a         5779 c         6246 g         5818 t
ORIGIN      
        1 gaggagactt ataatgagtt ctgataatga tatcgttttg actcataatc tattcgcctt
       61 atatatatct ggaggttttg aggatgatga ttaattatta tcgccagaac gaaatgtgca
      121 cgaaaaattg ttataagaat tgtgtcactt ttatcattgt gatatgacca cttcgtcatt
      181 aatctcatga gcgtttaaaa tactaccgcc aaatcacgca acgaactttg ctggattcag
      241 tttatgctgc tgtaaatccg ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt
      301 ggttctttaa gcgttttgct ggtgtactca ctacaaacga attgcgaatc gaatcaatgt
      361 gaaacggaaa ggtacaatct cccttttttt agtgttaaca tcgttcaacc gggtggaagg
      421 tgagaggtta atgtctttca tttgtgtacc tgattgcgaa ccaatttagc atttgttggc
      481 tagatggttt cgcaatgaac tgttaataaa caaatttttc tttgtatgtg atctttcgtg
      541 tgggtcacca ctgcaaataa ggatataaaa tgcctgttat aactcttcct gatggcagcc
      601 aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt ggtccaggtc
      661 tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct tgcgatctga
      721 ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt ctggagatca
      781 ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg ccgcatacca
      841 aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat cttgaccgca
      901 cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt gctgagaaaa
      961 actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc
     1021 gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat gacaagccag
     1081 gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc
     1141 gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca
     1201 acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa gcacttaacg
     1261 cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc
     1321 tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac aacgacggct
     1381 ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag taccagtatc
     1441 aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg
     1501 acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac tgcattaagc
     1561 cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc
     1621 tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca ggttcgctgc
     1681 atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc tgtactgaag
     1741 aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg tacagcactt
     1801 ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg
     1861 acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa aacaacatcc
     1921 cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt
     1981 atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc tccttgccgt
     2041 ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taagataccg gtaatgattc
     2101 accgcgcaat tctggggtcg atggagcgtt tcatcggtat cctgaccgaa gaattcgctg
     2161 gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt accgattcac
     2221 agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag
     2281 cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg cgtcgcgtcc
     2341 catatatgct ggtctgtggt gataaagagg tggaatcagg caaagttgcc gttcgcaccc
     2401 gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag ctgcaacaag
     2461 agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc ggaaaacgag
     2521 ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa
     2581 caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg gagaaagcag
     2641 aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg gtttgtcgta
     2701 taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa
     2761 agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tagtacagat gaaggcgact
     2821 atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa gccaaaatca
     2881 cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa gtgcttaatc
     2941 gcgtgaaaga cgatttgcaa gaactggcag tagtcgaatc cttcccaacg aagatcgaag
     3001 gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt caagtagcac
     3061 tatctgtgga gcctttgggc ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat
     3121 gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct
     3181 tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt cacattctga
     3241 ctaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg
     3301 atctgggctt ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt tttaactttt
     3361 taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg tgttattgca
     3421 cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc gcgttctcgc
     3481 gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta ccgtgaccgt
     3541 cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt
     3601 cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc tgttgaaatc
     3661 gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac cgctctggtt
     3721 gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt ctccctcttt
     3781 tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa taaaggtctt
     3841 ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc ttcttttttt
     3901 actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg
     3961 aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa agccagtcta
     4021 tcaggcgtaa ttgtcgcagc cagtctggac acggccagtg cggagaaacc ggagcgtact
     4081 taagtacgtg aggatttcga gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat
     4141 gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg aaaaccatgt
     4201 cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg tcagatgttg
     4261 ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta acccttcaga
     4321 tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg gttatcaacg
     4381 aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg gaaagcgctg
     4441 cactgaatgc gcgtctggcg gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg
     4501 aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg
     4561 agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat aacttcgatg
     4621 ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc tggtttgacg
     4681 ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg aaagcccagc
     4741 agccaccgat tcgtatcatc gcgcctggcc gtgtttatcg taacgactac gaccagactc
     4801 acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc agctttacca
     4861 acctgaaagg cacgctgcac gacttcctgc gtaacttctt tgaggaagat ttgcagattc
     4921 gcttccgtcc ttcctacttc ccgtttaccg agccttctgc agaagttgac gtcatgggta
     4981 aaaacggtaa atggctggaa gtgctgggct gcgggatggt gcatccgaac gtgctgcgta
     5041 acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg atggagcgtc
     5101 tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt
     5161 tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac tgtggttacg
     5221 cgaatgggtg aacccggcga ttgatagcga tacgctggcg aatcaaatca ctatggcggg
     5281 cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg tcgttggtga
     5341 agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt
     5401 cggcggcgat cgcctgctgg acatcgtctg cggtgcgcca aactgccgtc agggcctgcg
     5461 tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa
     5521 actgcgtggc gaaccgtctg aagggatgct gtgctccttt tctgagctgg gaatttctga
     5581 cgatcacagt ggcattatcg aactgcctgc ggatgcgccg attggcaccg acatccgcga
     5641 atacctgaaa ctcgatgata acacgatcga aatcagcgtg acaccaaacc gtgccgactg
     5701 cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga
     5761 accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta cagtcgaagc
     5821 gccggaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc
     5881 aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt
     5941 tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg ctttcgataa
     6001 agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct
     6061 gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg accacaacaa
     6121 ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg acgaaacgca
     6181 aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg
     6241 tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca
     6301 gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg gtgaggctgg
     6361 cccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca tcactctacg
     6421 tcgtagcaaa ctggatcgcc tgataggcca tcatattgct gatgagcagg taactgacat
     6481 tctgcgtcgt ctcggctgcg aagcgaccga aggcaaagac gaatggcagg cagttgcgcc
     6541 gagctggcgt ttcgatatgg agattgaaga agatctggtc gaagaagtcg cgcgtgttta
     6601 cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg gtactcaccg
     6661 tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag gctatcagga
     6721 agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc caggcgttga
     6781 agccttactg ctggcaagcc cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg
     6841 gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat
     6901 tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca ttcgtcagga
     6961 tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga acctggcaaa
     7021 agagaccgtt gatttctatg atttgaaagg cgaccttgaa tccgttctcg acctgaccgg
     7081 taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc cggggcaatc
     7141 cgcagcgatt tatctgaaag gtgaacgtat tggttttgtt ggagttgttc atcctgaact
     7201 ggaacgtaaa ctagatctta acggtcgcac tctggtgttc gaactggagt ggaacaagct
     7261 cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga accgtcgtga
     7321 catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg aatgtaagaa
     7381 agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt
     7441 tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca gccgtacact
     7501 cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt
     7561 ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg tcagaatatc
     7621 tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa ctgtttttcg
     7681 aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt tttggtaact
     7741 tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc gaggatattc
     7801 ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa agccgggtcg
     7861 aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct atgcggcctt
     7921 ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt cagcagaacg
     7981 gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg gttattatgc
     8041 ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga acaatggatt
     8101 tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca
     8161 cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag
     8221 gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa cggcgcaggc
     8281 gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg ggcgctaggg
     8341 ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt cgcccgtcgt
     8401 catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat ctgcagcgca
     8461 ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct gatgtactgg
     8521 atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct ggcattgatc
     8581 cccgtgttgt tgtggatctg ttgtcagtct aggccgatga atatgttagc acttggcgag
     8641 atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct ggtggcagcg
     8701 accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt tattggtctg
     8761 gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc
     8821 tgcgcgctgg caggggcgag cgcattgctg ctggccgata ttgtagcgcg cctggcatta
     8881 gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc
     8941 tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt aaaagctaag
     9001 atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg accgtagtga
     9061 aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg ctgttaattg
     9121 tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag aatattcaaa
     9181 aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag tttctggaac
     9241 aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg ggggtgacgt
     9301 tcccgatgtt cagtaagatt gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat
     9361 tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat gcccgtatgg
     9421 tcagcaaagg ccgtgcaccg ctgtaccagg atgatatttt atggaatttt gaaaaattcc
     9481 tagttggcag ggacggaaaa gtcatccagc gtttttcccc ggatatgacg ccggaagatc
     9541 ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg tgatgcagtt
     9601 acaggatgtt gcggaatcta cccgcctggg gccgctttca ggcgaggttc gggctgggga
     9661 aatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg cgcgaatggc
     9721 cggaatgacc agcggtaagg gaagcattca gttcgcgggg caaccactgg aagcatggtc
     9781 cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc cgccgtttgc
     9841 aatgccggtc tggcactacc tgacactgca tcagcacgat aaaacgcgta ccgaactact
     9901 gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggtcgtagca ccaatcaact
     9961 ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca
    10021 agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc ttgatgttgc
    10081 gcaacaaagt gcgttagaca aaattctgag cgcgctgtgt cagcaaggac tggcgattgt
    10141 gatgagcagt cacgatctca accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa
    10201 aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc caaatctggc
    10261 gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc
    10321 gaccatctga tttcattagt atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa
    10381 gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat cacagcactg
    10441 tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact ttctgattcg
    10501 attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac gccgtatcgt
    10561 tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc
    10621 gatcgtttcg atttgcatct gccccgtgaa accaaacaac aagcctctat tggcacgcaa
    10681 attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg ttccggacaa
    10741 aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc ctctaccagc
    10801 aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt ctggcaggcg
    10861 agacgaatct agtgtgtcga aaaattgacg gcgatgatac atcgccgtca atttactcct
    10921 gaatacagta gtattttgca gacaaaaatt ccgtaaatgg attattcgtt tataatcagg
    10981 ataatctaaa aaaaggcccc ctgaaaaagt ggttaacatg gaacaaatga aagttactcc
    11041 ttctgtaatt gttatgtctt atggcctcat cccttttagc cggatactga aaaacatcct
    11101 tcgagaggga cggttaccat gaagattttt ttggagaatc tttatcattc tgattgctat
    11161 ttcctgccga taagagataa ccagcaactc cttgtcggcg ttgagttaat tacccatttc
    11221 tccagtgaag atggcacggt tcgtatcccg acgagtcggg ttatagcgca acttaccgag
    11281 gagcaacatt ggcaattatt ttcagagcag ttggaattac taaaatcatg ccaacacttt
    11341 tttattcaac ataaactctt tgcctggctt aatttaacgc cacaagtggc tacattatta
    11401 ttagacaggg ataattacgc aggcgaacta ttaaaatatc cgtttattga gctgttgatt
    11461 aatgagaatt acccgcatct caatgaagga aaagataatc gcgatctttt gtccttatcg
    11521 caaatgtatc cccttgtgtt aggcaatttg ggagcaggta atagcacaat gaaagcggtt
    11581 tttgatggtc ttttcacccg cgtaatgctg gagaaaggaa caaatcacgc atcgctcctt
    11641 cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt gcatcatcgc
    11701 ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt tccatgctct
    11761 ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg ttcagcgata
    11821 accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca aacagatagg
    11881 atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc
    11941 actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg aactcgctaa
    12001 cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg gcggcgaaac
    12061 cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt tcggcgtctg
    12121 ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc ttgctgatgg
    12181 cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga
    12241 tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg cgatgcatta
    12301 tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg
    12361 ggaaacggtg gagccaggcg cgatgccgat gcgtgtggca ccaagtcatc tgcgctttgg
    12421 tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt tggctgactt
    12481 tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc gtctctggtt
    12541 taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg tcggctttgc
    12601 acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg attacgggcc
    12661 gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg atcatcaagg
    12721 gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc gtctggcgca
    12781 gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca gctatcagca
    12841 ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca tgacggagca
    12901 aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag agcgcagcga
    12961 ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct
    13021 acgtgatgag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg
    13081 tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa gcgttaaccc
    13141 agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag aaaagggtga
    13201 tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg acagagatga
    13261 tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct gctcgagtta
    13321 aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga tattcttgcc
    13381 cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg gtaattcgcc
    13441 ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc
    13501 tatccgtata acgcataaaa attaataata attgatataa caatgattcc tattatcaat
    13561 attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg cgcagaaatg
    13621 cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa aggcgagttt
    13681 ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg attgaccgta
    13741 agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac tttccgccat
    13801 aattccagca atctccgtag agccattgcg gatttgctga caaatatcct cacaaacttc
    13861 taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca ccagatgttc
    13921 aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg catgataatt
    13981 cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg tctgatagat
    14041 ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat
    14101 agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta ccggacagga
    14161 gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc caatcgcgcc
    14221 ccaactgatt aaatcagcaa taaactgacc ggtcaccata tcgaggaact cagtcgcggt
    14281 agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca gaccgtgatt
    14341 tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc cgacaacagt
    14401 tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt actggttgcg
    14461 cagcgactgc agacgggtgg cgtactccat tgcagcggtg agatcgtgga tcgagcaggg
    14521 gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa ttctgcggcg
    14581 ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg cgagttcggc
    14641 gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt tcattacaag
    14701 tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca tcacgataaa
    14761 ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat
    14821 aacaaaaaaa caaatcaatg cactagttct ctagtacatt cggcgactaa ggcccatgat
    14881 atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa tccacgggat
    14941 ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact gacgaagcga
    15001 ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat
    15061 agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca ccagattatc
    15121 catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca ttgccagata
    15181 cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct gagcctgatc
    15241 cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg
    15301 cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag caatcggcgt
    15361 tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga tatcctggca
    15421 aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg agtagaagac
    15481 cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc
    15541 acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg gaaattgtga
    15601 cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa tataaaaaac
    15661 gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata attctatgaa
    15721 agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata aaatgaacct
    15781 tcattttatt ttttatgaaa acagcatttc atttttatgg tttcgtttat accgatggtt
    15841 tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc ttaaaaagta
    15901 aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggtaaaat atgcaaagat
    15961 aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc
    16021 caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga atgatgtaga
    16081 cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt ccggaatggg
    16141 tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt ttctggacca
    16201 aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg acgatgttac
    16261 tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc ccttccagcc
    16321 tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg acgaaaacgc
    16381 ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt cctttgccgg
    16441 tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca
    16501 tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc agggttacga
    16561 tcaccgtggc gtggcgctct ccgccggtgt tcagcggatg gtgcgctccg acctcgcatc
    16621 ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg tgtttatcac
    16681 ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg atgagtttta
    16741 cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca ccatggggtc
    16801 gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga
    16861 agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag tgcaggaact
    16921 ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata ttgagtgggc
    16981 gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc
    17041 acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta tcgccgaagg
    17101 ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg acatcagcga
    17161 aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc cggactggga
    17221 accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc
    17281 ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag atgcaacaga
    17341 acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt
    17401 ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga tgccggatct
    17461 gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact tcgcctgcct
    17521 gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt
    17581 ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa acgaaatccg
    17641 cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc tgactgaagg
    17701 gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc tctctgattt
    17761 taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag atgaagagaa
    17821 cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc gcgactgctt
    17881 cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga ccaacgttga
    17941 gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg aagaactggc
    18001 gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt gtgaaatccc
    18061 gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct caattggctc
    18121 aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg tgtctgaatt
    18181 gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc gtgccgcgaa
    18241 gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg aagactttgc
    18301 cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca ccgtggtgca
    18361 aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg cgttcagtcg
    18421 ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga catcctgcgt
    18481 taatcgccgt ataatatctt tccttaacaa aaacttttgt attttatctg aggcagtgcg
    18541 cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga cccgtttacg
    18601 gctaaaaaaa gccaccatct cttccagcga taatgaatga tgcggcgctt tctgcacgac
    18661 ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac aggcatcgtg
    18721 aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat tttcgccgcc
    18781 gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct catccatacg
    18841 gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg ttaattcagg
    18901 ttcatcaaaa taccccataa acacattggg gccacgcgat gtttcttcac cttcgcaacc
    18961 tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg cggcagcgta
    19021 accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg catgcggcga
    19081 actttctgtg gaaccataaa cacttaataa tttaatgctg cgctgctggc attcgcgcgc
    19141 cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg aaaggtctgc
    19201 gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc ctagcataca
    19261 ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa
    19321 cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc ccgttgcgtg
    19381 accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc gcgcgcaata
    19441 agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg gcagaccctc
    19501 ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta tcgccgtggt
    19561 cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg cgggagccag
    19621 tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg gcaggattaa
    19681 atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg cctgacactt
    19741 attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca cggaaaccgc
    19801 accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag gcagttgaaa
    19861 tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca gacagctcgc
    19921 ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca cggcaatttt
    19981 gtctggcatc gcacgagcgg tctgctgtca gtaatcggcc agtgaagcat cgccccatag
    20041 cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca ctttcatttt
    20101 ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca tgattagccg
    20161 tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag aatagtgtta
    20221 ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa atgaatgttt
    20281 cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt acggaattca
    20341 ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac atggaattta
    20401 ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc gcggttgctc
    20461 ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt cccatgatca
    20521 ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc atgatgtcgg
    20581 ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc gggatcttgc
    20641 gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg tattgcgcca
    20701 gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg gcggcagcct
    20761 gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc aggcagaagc
    20821 ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc attgccagac
    20881 cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta atcagcgggc
    20941 gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg
    21001 cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg gtatagagaa
    21061 atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg gcaccttcct
    21121 cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata acctctttaa
    21181 caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag cggccaagca
    21241 ttgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca tccccagcct
    21301 gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg cctgggataa
    21361 actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga ttacgcaata
    21421 cggtataaga tgcgtgttgc ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg
    21481 cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct accggcgctg
    21541 atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca
    21601 tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc ccggctcgtg
    21661 ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg gcatcaaatt
    21721 tgtcatccga catgcgttat gctcccttcc cgctgcgttt acagcgggag atgtggttga
    21781 aaggggaatt ggcggctcaa ctggctaatc agggcaggga cgactttgta gatatcgccc
    21841 accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt gatggcgaca
    21901 atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat ccccagcgtc
    21961 aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc acgctccatc
    22021 cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag caccgccgcc
    22081 agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac gccaaccaca
    22141 cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc gcgacagaga
    22201 atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg agaggtatca
    22261 ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagcgg gctgttgatc
    22321 ttttcctgag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat gtgaccatcg
    22381 acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac acttaaccgt
    22441 gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg gtgtttatct
    22501 ttcagcaggg cagcaatact ttcggcgtaa ttttcagtgc gttgcagcgc gtcgttttgc
    22561 tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg gtcggtattt
    22621 tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag ttcagcataa
    22681 cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt ttcttctccg
    22741 taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg cctccggcga
    22801 atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa tgccgaccag
    22861 ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact gatttaccgg
    22921 ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt taatatcgga
    22981 ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat cttcaagcgt
    23041 gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat taatcaccgg
    23101 aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt cgccggaacc
    23161 ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca gcgcttttgc
    23221 ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca ggctgtgcgg
    23281 cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc cgccaacggt
    23341 cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa tggcattgag
    23401 atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac
    23461 aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca tgatttctcc
    23521 tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa aatagtgtgc
    23581 gatagcggtg cggggggttc gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa
    23641 aaaatgcgat tgctaaaaca aaagtgcaat ttgcggcaat ata
//
LOCUS       DAJTYG010000058        23136 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTYG010000058 DAJTYG010000000
VERSION     DAJTYG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.058"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..144)
                     /locus_tag="OO568_002456"
     CDS             complement(<1..144)
                     /locus_tag="OO568_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6641776.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVL"
     gene            complement(413..1162)
                     /locus_tag="OO568_002457"
     CDS             complement(413..1162)
                     /locus_tag="OO568_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6641777.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(1252..1425)
                     /locus_tag="OO568_002458"
     CDS             complement(1252..1425)
                     /locus_tag="OO568_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641778.1"
                     /translation="MSEFDTQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            complement(1573..3547)
                     /gene="pdeR"
                     /locus_tag="OO568_002459"
                     /pseudo
     CDS             complement(1573..3547)
                     /gene="pdeR"
                     /locus_tag="OO568_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            3601..3703
                     /locus_tag="OO568_002460"
                     /pseudo
     CDS             3601..3703
                     /locus_tag="OO568_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3781..5715)
                     /locus_tag="OO568_002461"
     CDS             complement(3781..5715)
                     /locus_tag="OO568_002461"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6641779.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(5783..6910)
                     /locus_tag="OO568_002462"
     CDS             complement(5783..6910)
                     /locus_tag="OO568_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR6641780.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPAIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(7054..7842)
                     /gene="fabI"
                     /locus_tag="OO568_002463"
     CDS             complement(7054..7842)
                     /gene="fabI"
                     /locus_tag="OO568_002463"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6641781.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            complement(8210..8563)
                     /locus_tag="OO568_002464"
     CDS             complement(8210..8563)
                     /locus_tag="OO568_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6641782.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            complement(8631..9437)
                     /gene="sapF"
                     /locus_tag="OO568_002465"
     CDS             complement(8631..9437)
                     /gene="sapF"
                     /locus_tag="OO568_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6641783.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(9439..10431)
                     /gene="sapD"
                     /locus_tag="OO568_002466"
     CDS             complement(9439..10431)
                     /gene="sapD"
                     /locus_tag="OO568_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6641784.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(10431..11321)
                     /gene="sapC"
                     /locus_tag="OO568_002467"
     CDS             complement(10431..11321)
                     /gene="sapC"
                     /locus_tag="OO568_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6641785.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(11308..12273)
                     /gene="sapB"
                     /locus_tag="OO568_002468"
     CDS             complement(11308..12273)
                     /gene="sapB"
                     /locus_tag="OO568_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6641786.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(12270..13913)
                     /gene="sapA"
                     /locus_tag="OO568_002469"
     CDS             complement(12270..13913)
                     /gene="sapA"
                     /locus_tag="OO568_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6641787.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(14226..14471)
                     /locus_tag="OO568_002470"
     CDS             complement(14226..14471)
                     /locus_tag="OO568_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6641788.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTHLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(14605..15990)
                     /gene="puuP"
                     /locus_tag="OO568_002471"
     CDS             complement(14605..15990)
                     /gene="puuP"
                     /locus_tag="OO568_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6641789.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(15980..16144)
                     /locus_tag="OO568_002472"
     CDS             complement(15980..16144)
                     /locus_tag="OO568_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641790.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(16293..17711)
                     /gene="puuA"
                     /locus_tag="OO568_002473"
     CDS             complement(16293..17711)
                     /gene="puuA"
                     /locus_tag="OO568_002473"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6641791.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIDQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            17923..18687
                     /gene="puuD"
                     /locus_tag="OO568_002474"
     CDS             17923..18687
                     /gene="puuD"
                     /locus_tag="OO568_002474"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6641792.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            18714..19271
                     /gene="puuR"
                     /locus_tag="OO568_002475"
     CDS             18714..19271
                     /gene="puuR"
                     /locus_tag="OO568_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6641793.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            19421..20908
                     /gene="puuC"
                     /locus_tag="OO568_002476"
     CDS             19421..20908
                     /gene="puuC"
                     /locus_tag="OO568_002476"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR6641794.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYAAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            20910..22190
                     /gene="puuB"
                     /locus_tag="OO568_002477"
     CDS             20910..22190
                     /gene="puuB"
                     /locus_tag="OO568_002477"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6641795.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            22228..>23136
                     /gene="gabT"
                     /locus_tag="OO568_002478"
     CDS             22228..>23136
                     /gene="gabT"
                     /locus_tag="OO568_002478"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCR6641796.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLA"
BASE COUNT         5784 a         6034 c         6017 g         5301 t
ORIGIN      
        1 cagtactgca ccgcctaatg cccctgcacc cgcgccgatt gcggtgttgc ggtcccgttt
       61 agaccagtta gaacaggcac tcagagacat tgccaaagta attgccagaa cagccgcggt
      121 catttttttg ctcgttacaa acataatact ctctcctgaa tttatgattc acggaagtaa
      181 gctctctata actataatag ctaagaataa gcctggtgaa attattccgt gaaatctgct
      241 cgcgcgcagg gttatatcac gcacgtgata attacttcct gttatatcgc tgataataat
      301 tttatatctt gagagtgtta ataacaggta aatagtctta attatcaacc aggaatcatc
      361 ttagagcgga tgatttgcca aactgcaaat catccgtaga gaagggaaat ggttaaatgt
      421 caacgacgtg aacggtgagt ttactgtgct caagatgcat cagatcgctg gcgcgtattt
      481 tcgaatcggt aatcacgcga ttgaatcgct caacgggacc gatggagtat gaatgtacag
      541 caccaaattt cgagctgtca gtcaggacta tcgcttcgca ctctttttcc agcacggcat
      601 tgaccacatc ggtgcgcatc atatcgcgac cggtgaatcc agtttcaggt tgccagccat
      661 caataccaat aaatgctttg ctgaaatgta cctgctggat gcactggcgt gtcaaagggc
      721 caaccatact ttcgcttttt ttctggtaca cgccaccgag caaaatgact tcacaaggcg
      781 cgtctttcag caaatgcgcg atgtagctgc tgaccgtgat gatagtgaca tttttcttct
      841 gctcccccag agtccgggcg agcagggcat tgctgctgcc attttcgata aagatggttt
      901 cgcccggttg aaccagtgac gcggcaaact cggcgagttc acgcttcagc gtatagttgc
      961 tcatcatacg ggtttcgacg tcatcactat caagcgaaac tgcaaagcca tgtgcacggc
     1021 ggaggtaact cagtttttcg agggtgttga gatcctggcg aatggtaact tcagaaactc
     1081 cagtggcttt tgccagatcg gttacgctaa cctgaccctg gtcaatgacc atctgtagaa
     1141 tagtttgttg tcgggaattc atcgctgtaa ttcttataac gttataatac taaataaaaa
     1201 aatactgacg cgccgggcat cctcaaatag gaaaacctga aggcacgatt gttaaatctc
     1261 ccacggtgtc ttcgggatgt ctggcgtaga ttcagcaacc tggcgcaaga gttctgcttt
     1321 aagtatctcg agattatgga tcgcagagtg gtaatcgcct gccacaagga tatctaacac
     1381 aatatcaata cgttcggcaa cgcgctgggt atcaaattca gacatcaagg acatccttat
     1441 agcaaaaggg ggaaaatgaa taatgcaaaa attgccgcta aaagagaagt gtttaacagc
     1501 aacggctaat tatcatccag gaatacgata tataatgacg ggatatagcg ctaagtatat
     1561 atattcatct acttatgcgc gcttcagata gcgtttatac cagcgttcga aggcgacggc
     1621 gggcatcggt ttggcaaaca aaaatccttg ccgctcattg atcccgttct tggttaaaaa
     1681 agcatcttcc tttgcactct ctacaccttc ggcgatcacc tgaagattca atgcctgggc
     1741 cacagcgacg atcgcccgga ccagtgactg cgagacaggt tgtttgtgaa tatctcgaac
     1801 aaaaacctgg tcaagtttga tggcatcgat cggaaagcgc gccagttgcg aaagtgaaga
     1861 gtagccggta ccaaaatcat ccagatgcac ttgcgcacct agttggctaa attgttgaat
     1921 aacagacagt gccagttcat cattctcaat cagacaactc tctgtcagtt caacatctat
     1981 agggcagtat tcaaaattga gttcctggag aacctgtttc agggcggtga aaatggtttg
     2041 atcggcgagc tgacgtgcag aaatatttac cgccactcgc aggtttatgc ctttatcccg
     2101 ccactttgcc acctggcgta cgacatcgag aatcacccaa cggcctaaag gcacaattag
     2161 ccctgactct tcggcgtagg aaatgaagtc caacggtgga atcaacccac gttcaggtga
     2221 ctgccaacgt actagtgctt ccagactgcg cacttcgcca cgctaggtga ttttcgataa
     2281 ccaactgatc gttttccagt gctttacgca agttggtatc cagccagaga tattcaaata
     2341 cccgctgatt catttctgga gtaaaaacgc aaaattgtcc tcgtccgcct tccttcgctg
     2401 tgtacattgc tgtgtcggcg tgacgaataa tagtcgcgct gtccgaacca tgttcgggag
     2461 agagcgaaat acctactgaa cagctggtat aaacttcaat taaaccaata cgaaagggga
     2521 ggcgtaagcg ggtcaaaatt cgtgatgcca ttgcttccag cgcgctttgt gaggtattgg
     2581 atgccagtac cagaaactca tcgccacctg gacgcgccaa cacctggtca tgttcgagac
     2641 agcttaaaat agccaatgac acgtcgcgta ataactggtc accaaacaaa tgcccatagg
     2701 cgtcgttgac ctttttaaaa ttatccaaat caagatacac aaccccaact ttattgttat
     2761 ctgcctgatt aatagcgtga tcgattaact cctgcattgc gttacgattc ggcagtccgg
     2821 tgatactgtc ggtatttgcc agaatacgca gtcgctcctg agtgcggcgc tcttcggtaa
     2881 tgtcggtacc ggaacagatt aaaaaaatct cgtttttgcc actgccgctg tggacaaatt
     2941 tattgcgaaa cagaaacagc cgctggcctt tacgtgttgg tatccacagt tcgacttcat
     3001 atgcattgcc gcttcgaaaa aatacacggt tattgcgcct ggatgccgca gcttcacgac
     3061 ggctcataaa cagtttaaac acgctttgcc caatgacgtc gtgttctttc aaccctgtgt
     3121 aatcttcaca taaccgattg aagcgttgga tattcccccg gctgtcgaga atcactattg
     3181 cggaatgtgc ttcagaaact acctgctcag caaaggaaag cccgtgtgat aaatcacgag
     3241 caaccgccgg tgtgtcatgc catgcagacg cgttgccagc ccattcccgt ttattgattt
     3301 ttcgtcctac cagatgctca gaaagatcgc tttcatcgac ggtcagactc atcatcaggc
     3361 tggaggtgat gaccgtcatt tccctgatgc gagcagcctg ttcggcagat aactgcaatg
     3421 tacgatcagg ttctgtggtt ttggtggtag aaaagcgcaa agcatcgctg ctttccgtca
     3481 accgccagta tggattgtgc gatccgagaa agttgtacaa cgttgtggac tccctaacgg
     3541 ttttcatgat gcatatcctc cggttaacag cggagtccgc ggttaaataa aaggaacaac
     3601 atgacttaca tgaaattaac ggcggataaa cgcattgttt tttatatatt tatttgtaat
     3661 ccagttttgg aaaaacgcca gttttcaaac gaaagtcagt taaaaaatct gcctggatat
     3721 aacgaaggta gagcggggaa ataaacggcc catccatgag gaatgggccg tgaaaggaga
     3781 ttacgcgacc gggcgcgcaa taatgctgcg ggtttccatg cggacttcgg caatggtgac
     3841 gtcaataacg tcagttactt tgtaaaccgt ttcaccttta atttgtacag tgccgttttc
     3901 ctggctgcaa accagttcat cgcgcacagc gtgtaagaaa ggtgccggaa taaaggcgat
     3961 agcgccgtta tcaaccaaac gcacacgcat gccgccacgg ctgatatcga caatttccgc
     4021 cgcgaaacgg gtgccggtcc cggctttatc tttcaggaag cgtgcgtata accagtcgcc
     4081 aacatcacgt tctgccatcc ggttgagacg gcgacgttcg gccatttgga cagtgatttc
     4141 atcctgcgga cgcgtcgcag tttcgccttt gataaccgct ttcagcagac ggtggttgat
     4201 catgtcgcca tatttacgga tcggcgaagt ccaggtggcg tatgcttcca gaccgaggcc
     4261 aaagtgagga ccgggttcag tgctaatttc agcaaatgac tggaagcgac gaatgcggct
     4321 gtcgaggaaa ccagttggtt gcgcatccag ttcacgacgc agtttgcaga aaccgtccag
     4381 cgtgagtact tcttcggcat cgacatgcag accgtgcgtt ttcagcaacg ctgccagcgc
     4441 gtcggcattc gccggatcaa agcccatatg cacgttatag atgccaaaac cgagcttatc
     4501 gcgcagtacg cgggccgcac aaatgttagc ggcaatcatc gcttcttcga cgatacggtt
     4561 ggcaatgcga cgaggctcgg cgacgatatc cagcacttca cctttttcac cgagaataaa
     4621 gcggtaatcc gggcgatctt taaacactag tgcgtggtta tgacgccact cgccgcggcg
     4681 ttggcaaatt tgcgctagca aacggacttg ttcggcaatt gcttcacttt caggcttcca
     4741 gtcaccggta ttttccagcc agtcagaaac ctggtcatac accagcttcg ctttggattc
     4801 gatggtggcg gcaaagaatt cgatattatc ttcaatggtg ccatcagcgg agagcgtcat
     4861 gcggcatgcc agtaccgggc ggacttcatt ggcgcgcagt gagcagagat cgtcagaaag
     4921 ctcgcgaggc agcataggga tgttgaagcc aggcagatag ttggtgaatg cgcgaatttt
     4981 cgcggctttg tccagcttgc tgccttcagc aatccacgcg gttggatcgg caatcgccac
     5041 aatcagctga agtttgtcat ccggcagcgc cttagcgaaa agggcgtcat ccatatcttc
     5101 agtgctggca ctgtcgatgg tgacaaaatc cagcgaggtc agatcttcac gaaccagacc
     5161 ttcatcgagc atttcggtag cgacgccgtc tggtgcttct ttttccagat tatggcgcgc
     5221 aagggtaacc caccacggta caaagtggtc gtcgccaaaa gtgatgtatt gtgtcagttc
     5281 agcatagaaa gaacgatcgc ctttcagcgg atgacggcgc atttcggcaa ccgcccagtc
     5341 gccttcttta aactcgtggt tcaggccacg ggctgcgcgg caaggaatgg cgtcttttaa
     5401 aagtggatga tcaggaacga tggtcagacg gtcatttttg ccctgaactt tacccacgaa
     5461 acgagtcagg aacggttcaa ccagttcttc tggctctgcg gattcacgtt ctttttcact
     5521 gtggatcacc gcgataattc ggtcgccatg catgactttt ttcatctgcg gcggcggaat
     5581 gaaataactt ttttgtgcgt cgacttccag gaagccaaag cctttttctg tggcttttac
     5641 caccccttca gcgcgtggcg tctgggaatg cagttgctgt ttaagctgcg ctagcagcgg
     5701 gttgtcctga aacataattg tctattttgg tggccattag agcggctgac agttttacgc
     5761 gaatctgtct gacgcggcaa ggttaatatg tctcacccaa cgcgattttt aagcgattta
     5821 tccagccaca cagcccgctc cataccagca gattaataat ctgcgttgat gatttttcgt
     5881 gttcgagtaa agggctgaac tgggcggcgc taaagcgatc gggtgctcgg gtcagccatt
     5941 gtatcgcctg aacggtagcg cgctcaacag aatgacggtc agcccatcgg ttaatttcat
     6001 tttcgccctt gcgcagaagg gtgctgattt ttttatattc atttgatgaa tccatacagg
     6061 tggaaaaaca agcaggactg ccatttatac gcgcgcataa caacgccgca agctgattat
     6121 ccggctgtgt attgacccgt gtgctgttta acaacttttc gagaagggaa agtgaaatag
     6181 ggtggagaga gtgtgtttcg gccagttggc aaagttctgc agttgaactt tttgtgtgct
     6241 cttcaggtag tttttccacc tcatagctgc ttcgccagcg tattgattca tcagcaaaca
     6301 gtgacgcgtc ggcgtagttt tgtatcgcca gcccgggtaa ccagcgtacc ggatgcccga
     6361 gataagcctg aaatgtcgca attgtccgcg cctgaaagcc aataaaacca atgatttggt
     6421 taatgatcac acagtcccag acagtaagac cgacgtcatt cagatgctgg cgcgatcggt
     6481 tgtcgataat agcaggcgag ctagccagtt ggcgggcata ttgcgtgatt tgtgccaacc
     6541 gatgattact ttccctggag gaatcaggtc cggggagcgg cgtaagtcgc gccgaatagt
     6601 agttacataa ccgctggacg ccgcaaacct gagcaaccgt cagggccgtc gacaagcgtt
     6661 cataagcggt gaaggtttgt gaacgggtca cggtaagctg gtcagggaac aactccactg
     6721 caagaacgcg cgcagggacc agccatgaaa ggaagggggc cagcgtctct tcatcaagga
     6781 tcacgtcgag cagaaaggga tcgctgacct ttgcggcttc cgggaccaga ggcagaacgt
     6841 catactccgt agtactggat tgcgtttcat gataccagtg gcttttgccg gtgatgtggc
     6901 gttgttccat gggcgctcct tggtcgtaaa ggaaatcgtt atcctgacgc aaggcgggaa
     6961 ggggagaaag acggatcggc gataacaaat atcagaaagg tataacagag ataacgggcg
     7021 gcagaacgcc gcccatcttt accaacagaa cgattatttc agttcgagtt cgttcattgc
     7081 agcgatgctg aaaccgccgt caacgtggac tacttcaccg gagataccgg cagagagatc
     7141 ggagcacagg aatgccgcag agttacccac atcttcaata gtaacggtac ggcgaatcgg
     7201 ggtaacggct tcgcaatgag ccagcatttt gcggaagtct ttgataccgg aggccgccag
     7261 agtacggatc ggaccagcag agatggcgtt aacacgcaca ccttccggac ccatcgcgtt
     7321 cgccatatag cgcacgttcg cttccagaga cgcttttgcc agacccataa cgttgtagtt
     7381 cgggatagcg cgctcagcgc caaggtagga aagggtcagc agggcagaac ccggattcag
     7441 catggagcgg caagcttttg ccattgcaac gaagctgtag gagctgatgt cgtgggcaat
     7501 tttgaagcct tcacgggtaa cggcgttaac atagtcacca tccagctgat cgccaggtgc
     7561 aaaaccaata gagtgtacga aaccgtcaaa tttcggccaa actttcccca gttcagcgaa
     7621 catggtgtcg atgctggcat cttctgcaac atcgcactgc agaacgatgt cagaacccaa
     7681 ttgagcggca aattcttcta cgcggccttt cagtttgtcg ttctggtagg tgaatgccag
     7741 ttcagctcct tcgcggtgca tcgcctgagc gataccgtag gcgatggaca gtttgctggc
     7801 aacaccggtt accagaatgc gcttaccgga aagaaaaccc atagctttaa tccttattgt
     7861 tgatgcttgt tgtgcctgaa aatcaggcga ttcgtcgttt tagtaaacag tacgaacaga
     7921 taaacggtta ttataatcaa cctggctgtg agtagctata gttgccaggt ccgaccggag
     7981 caggctgcgg cagggggggc gcttttcccc ttaccctaac cctctcccca gaggggcgag
     8041 gggaccatat tgtgcaaata ttgttacccc agcaacaaac aggctcatac agcccctaac
     8101 ccttttatgg cgatggctgg gatggttcag accttgccga atattctcca gcactgtctc
     8161 catgttttca ccacaacagc tattcggctc ggtcggcccc ctcgcccttt cagggtgagg
     8221 gtgaccggcg gttcagttcc tgcagaataa tctccagcac cgcctcctca ttacaatcaa
     8281 tctcgttatt ccagaaacgc agcacggtcc agccctgcga ctcaagccag ctagtgcgcc
     8341 tggtatcata ggcaactgcc aaatcatgct gcccaccatc cagctcaacg actacgcgcg
     8401 ccgagcagca agcaaaatcg agaatgtagc tccccaccgg atgttgacgg cgaaatttga
     8461 aatcaccaaa acgtcggctg cgaagatatc gccagagctt tcgttcctgc aaagtgagat
     8521 tgcgtcttaa atcacgcgca tttgatttta ttttatccat cacctcattc tgacaagatt
     8581 taatctcttg tcagaatgag gtgaataatc tggaaggagg attcagaaaa ttagcgatct
     8641 ttacgccacg catccgctgt caatgcttcg ccaaagtgcc cggcaatcag ccgtttggtg
     8701 agttcatgca gcggcgatgc cagcacatcc gcggtgctgc ctcgctcgac aacctcgccc
     8761 tgatgcatca ccagcacctg gtcgctaatg tgcttcatca ttccgatatg ctgggtaaca
     8821 taaatatacg aaatgccctg tttttcctgt aactccagca tcagattaat caactgcgaa
     8881 cgcatcgaca tatccagtga agcgagggct tcatcggcaa taatgacttt tgggcgcaat
     8941 atcagcgcgc gcgccagacc cagacgctgt ttttgtccgg gtgccaacat atgcggatag
     9001 taactgacgt gatccggtag cagcccaacc atacgcatcg tttcaataat ctgtttgcga
     9061 cgctgttccg gttccaggtc ggtgttcagg cgcagtggaa aatccagaat ttgtgagata
     9121 cgttgacggg gattcaacga ggtcgaagga tcctgaaaaa tcatgcgaat acgctgacta
     9181 cggaaggaat aatcgccaaa atgcagcgga tgatcgtcaa tcaataactc gccgctggta
     9241 ggttctatca ttcccgccag catttttgcc agtgtggatt tacccgaacc attctcgcca
     9301 ataatcgcca gtgtctggcc ttcacgtagc gtaaagctca agggttttac cgcttctacg
     9361 gtctgacgac gaaaccaacc ggtccggtag cggaacgttt tacttaaatt acgcacttca
     9421 agcagcgttt cgatcatctc actctttctc catgttcagc gggaaatgac aggcatagag
     9481 atgatttttc gcccccgtca aacgtggcgt cacaatgcat tctcgttgtg catacgggca
     9541 acgtggcccc agacgacaac caatcggtaa ctgttccagc aatgggatag cgccgggtag
     9601 agtattgagg cgacttttat gcggcatcgc gctgccgaag tctggtatcg cgcggatcag
     9661 cgcctgggta taaggatgat gtggcatcgt caccaactcc ttactcggcg cggtttccac
     9721 tgtttgaccg cagtaaagca cgttaatttt atccgcccat tggctaagca tttgtaagtc
     9781 atggctgata agcaaaatag tggtattgct gttttggttg agacgcgtca gcaggcgaaa
     9841 gatttgcgcc tgggttgttg gctccattga gtttgtcggt tcgtcagcaa tcagcagacg
     9901 cggttgattc gccagtgcaa tggctatcat cactttctga cattcacctt cggtcaactc
     9961 atagggaaaa ctgcgcatcg catctttgtg atctttaatc cccacgcggt gcagcagttc
    10021 aatggcacgg cgtttgcgcc agccaaaacg ctgccaccaa cggcctttat aggtccaggc
    10081 tgggatgttt tgcatcaact ggcgacccac acgttctgaa gggtcaagac acgactgcgg
    10141 ttcctggaaa atcatcgaca cgttatggcc aaccagtttg cgccgttcgc gtgcggagag
    10201 acgcagcaaa tcgatatcat caaaacgcat acggtcagca gtaacacgcc agttatcttt
    10261 attcacccca caaatcgctt tcgcaatcaa actcttgccg gaaccggatt caccaacaag
    10321 accgcggatt tcaccttcgg ttaaggtcat acttacccgg tcgacggctt taacccactc
    10381 atcaccggtt ttaaattcaa tggtcaggtt acgaatatcg agtaatggca ttattccacc
    10441 cccgcaataa tcgcacgacg aacgccatcg ccaaggaggt taaccaacaa cacgctaatc
    10501 ataattgccg cacctggcag catgacagtc cacggggcga cataaatcag ttccagcgca
    10561 tcaccgagca tcgctcccca ttcaggcgag gggagttgtg cgccgagatc gagaaagccc
    10621 agcgcggcga tatcgagaat tgccatcgac agtgcgcggg tgatctcggt gaccagcccg
    10681 gcggtgatgt ttggcatcac agcaaaccag agaatattca gcgttgatgc gccatccaga
    10741 cgggcggcga taacgtactc tttttccagt tcgtcatgca ccatgctgta aatcgaacgt
    10801 accatacgcg gcagcaatgc cagccagaca gcaaacatag cgtgggacaa actcggtccg
    10861 gcaaacgcta caacgataat tgccaacagc agcgaaggga tcgccagtaa tgtatccaga
    10921 atatggttaa gcaccgctga gcggaggccg tgtgtcgccc cggcgaaggt gcccagtacc
    10981 aggccacaga tcgtcgcggc aagcgtcacc acaaatgcgc cacccacggt tggcgcagct
    11041 ccgctcagta aacggcttaa aacatcacgt ccgaggtcgt cagtccccag gaagaaagaa
    11101 acttcgccat agcgcgacca tgatggcggc aataattgat aaccgagaaa ttgctggtcg
    11161 atgccgtaag gcgcaaacca gccgccaaaa atacacagta cagccagtcc cgcgcagccg
    11221 tacaggccga ccatcgcaga ggcgtcacta taaaatttgc gccaggcggt acgcagcgtg
    11281 cccggcgggc gcttttcgct gtatacgcta tcgtaaggca taccattcct tatgtttcag
    11341 agggttagcc atggcaccca aaatatcaga aatcacgtta acaataatga ccagtgagcc
    11401 acacaccatc actccggcgg aaatggctgc gtaatcctgc tggcggatgg cgttaattaa
    11461 ccagcgccct aaacccggcc agctaaaaac catttcggtg atcatcgcca gcgtcagcat
    11521 ggtggaaaac tgtaagccca ggcgaggaat aaccggcggt aacgcgttat gcagaacgtg
    11581 gcgacgcaaa atagtaaagc gcgacaaacc gcgggtcgcc gccgctttta catagttctg
    11641 gtcatacact tcaatagtgc tgatacgcat caggcggatc acttcagttg ttggcgcaac
    11701 cgacagggtg atcacgggca atatcatatg gcggattgcg ctcatgatca tttcatcccg
    11761 ccatggtgag tccgaaagcc aggcatcaat caacgcaaaa ccggtaatcg gtttcacttc
    11821 gtagagcaga tcgaaacgcc ctgaaacggg caaccaacct agcgtgagtg aacaaaacag
    11881 tgtcaacaga agcgccagcc agaacaccgg gattgaaaag cccagtaagg cgatggcgtt
    11941 gatcaagtta tcctgccact tatggcgcgt aatcccggca atcatcccca cggggatgcc
    12001 aacaatcagc gcaaaaccga aggcgaggat gcacagttct atcgtcgccg ggaagacctc
    12061 ttttaactgt tcggcaattg gttgaccgtt aatgctggaa acaccaaaat cccagtggat
    12121 caggccatta aaccagaaca cccaggcatt ccacagtgac gcgccttgta acggcgcgtg
    12181 tggagtgaaa tagctcaagc tgaagccaac gaaggtcagc aggaacaagg tgacaatcaa
    12241 taacaaaatg cggcgtaagg tgaagataat catggttttt tcacctcatc ctgtttctcg
    12301 cgatacaccc cagcaaagga ggcgttacca aacgggctaa gtaccagacc tttgatatcg
    12361 taccgatagg cctgcaaacg caatgacgac gccagcggca gaataggtaa ttcctgcgcc
    12421 agaatactct gcgcttcgtc ataggcttca atacgcgctg ccagctgctg cgaggagagc
    12481 gccttacgca acacgctgtc gaatttcgga tcgcaccagt gggcgaggtt ggtctgtgaa
    12541 tgaattgccg cgcagctcag taacggacgg aagaaactgt ccgggtcgtt actgtccgtc
    12601 gcccaaccgg ataacgtcag atcatggctc atatccatca accgcgcctc ctgaaagcga
    12661 ccttctaccg gcacaatcac cacttttacg ccaacctgcg ccatatccgc ctgaatcagt
    12721 tcggcagttt tcagtggact ggggttccac gcctgcgaac gtgtgggcac ccacagtttc
    12781 agcgttaagt tttccagccc caacgccttc aactgttcgc gcgatttcgc cggattgtat
    12841 tcagtaattt tagcctcgtt gtcataggcc cacgaggcgc gcggtaaaat agaggccgcc
    12901 gtttcagccg taccataata gatggattgc atcaggcgct ggttattaat cgccagtgcc
    12961 agtgcatggc ggacagcggg attatttagc ggcggtttgg cggtgttaaa tgccagatag
    13021 gcgacgttca tcccaggacg cagcgttaaa cgcaagcgcg ggtcgtcacg caaaatggat
    13081 agctggctgg cagcaggcca ggccagaacg tcgcattccc cggtcaggag tttcgacaga
    13141 cgtccggtgc cgccggagcc taaatccacc actacctgcg gcattaacgg tttaccgcgc
    13201 cagaagtcat catgacgttg taggcgaata aattgcccgg cgcggtattc cgacaactga
    13261 tacggaccgg tgccgaccgg ttgacggtcg agttgctcct ggcgatcttc tttctctaac
    13321 ttccgggcat attctgccga catgaccgaa gcataatggg ttgcgaggtg ccacaaaaaa
    13381 gaagcatccg gctgggcgag tcggaactca acggtatgat tatccagttt gcggacgctt
    13441 ttcacgttat cggcaaattg caggctgtcg aagtagggga agttgctgcc gttgacgtta
    13501 tgccacgggt tgttgcggtc aaaaattcgc tggaaggtaa acaccacatc gtcggcattc
    13561 attttacgag tgggagtaaa ccagtcggtt ttttgaaacg gaacatcgcg acgcaggtgg
    13621 aagcgatagg tcgcgccgtt gtcgagtact tcccagcttt cggcaagttc cggcatcagg
    13681 cgataggtat agggatcgac atccagcagt cgatcataaa actgggcggc aagggtatcg
    13741 acaattaacc cactgctcgc tttggatggg ttaaaggtgt tgacttgccc gctgacgcaa
    13801 tagacaaaac cgctgtcgcg gatatcagca tgcggggggg attcaggcgc ggcgattgcc
    13861 tgaccactca caagtccagc aatcaccaaa agagacgata atacctggcg cataatatta
    13921 agggatttta tgtaaagagg ctatcttact aatatttaat gacatttgcc attaccgttt
    13981 gtgttcaggg gtcgtaatga gggccgtgtg gttggtcgtt gaacaggtgg cgtcgccatc
    14041 tattcaacat tcgtacccgt gatttctcta taactataac tcacagaaca acttagcgag
    14101 gagcagagcg gtacgatcgt gagaaaccca ttaaagcacg ctgcagtgcg tgtcattgtt
    14161 agccggatgc ggcgtgaacg ctttatccgg acaacgatac tgaccgatcg tctgcaatta
    14221 aataattact cattacccca ttgattcaaa aactctgcga tctcatcaat gcgtacggga
    14281 ttaatccccg cttcagcagc catttcatgt tgggcttctt cgctgatctc ttcattgttc
    14341 atcaaatggg tgagcagtaa ctggaagtag tgggccagcg gtgtgcgttc agcctctgcg
    14401 acaggctctg cgcattcggt cgcgtactca tcggcaatat caaaatattt tagcgggata
    14461 tcgtggttca ttatttgccc ctggggttaa tgcgctaagc ataagggcgg atgatagcac
    14521 tcgtgatccg ggtcatacat ctttttatag ttaatggcgc ggcgcgttac cctcaggcag
    14581 gataatgcgc cgcgcatccg gctattacgt ttcgctcacc ggcgttctgt caccttcgta
    14641 cagcggcact ttgcgatagc ggcggatcag ataccacaaa tatgcgccgc ctaacgaagc
    14701 ccataccaga ccgagtgtca gtgacgttga ctctaggtta acccacagca cacccaccgt
    14761 cagcgcgcca accagcggca tcagcaaata gtggaagtga tccttccagc ttttattcat
    14821 tcctttacgc cgccagaaat gattaaacac cgacagattc acgaaggtaa acgccaccag
    14881 agcaccgaag ttaatcagtg ctgtcgcggt gactaaatca aagaacagcg ccgacagcgc
    14941 gacaatcccg accataatga cgttgagtgc cggagtccgc cattttgggt gcacatagcc
    15001 aaacacgcgc tccggaaaca cattgtcacg ccccatcaca tacagcagac gcgacacgct
    15061 ggcatgtgaa gccaggccag acgctaatgt gttcacaaac gtggtgcaga ggaaaatcga
    15121 ctgaaagagc ttgccgccga cgtacagcgc aatttcaggc agtgcggcgt ccgggtcttt
    15181 aaagcggctg atatcgggaa agaacagctg cataaagaac gacgccgcga taaagataac
    15241 gccgccgtat accgccgtca ggaagatggc tttcgggatc acgcgtgcgg cgtccggcgt
    15301 ctcttccgaa agcgtggtca ccgcatcgaa accgaggaac gagaaacaga caatcgtcgc
    15361 accggtaata atcgggatca ggtgcgcgtt ctcgctgata aacggctgaa gtgaccagac
    15421 ggtgccaacg ccttctcctt tatgcagtcc ctgaaccacc aggaagataa acaccaccat
    15481 gatggagatt tgcaccagta caaacagggt attgaagtta gcgaccaggt tgacgctctt
    15541 cagattcgcg gcggttaaaa tggcgacgaa ggttaccacc cacacccacg gcggcacttc
    15601 cgggaagagg gcggagagat agattttcgc caacaaaacg ttgatcattg gcaaaaagag
    15661 ataatccagc agtgatgacc agccgaccat aaatccgacg tgcgggttaa tcgacttttg
    15721 cgcgtaggta taggccgaac cggcctccgg aaactggcga accagtttgc cgtagctgat
    15781 agcggtaaac agcacgcccg ccagcgccag caaataggac gccggaacgt gaccgtcgct
    15841 aatgccggag acaataccaa aagtatcaaa tacggtcatc ggcgtgagat aggccagacc
    15901 catcattacc acctgccaca atttcagtga ttttcgcaga cgggttttgc cgggttgcgc
    15961 agcaatattc agtggtgaat taatagccat aattgctgcc tcccctgcgt aataaggttt
    16021 gcggtcgtgt cgacctgaaa tcaaacctgt ttagcaaggg aagcaaaggg ggcggagtcc
    16081 agtcagtagc aggaggggcg catacgcagc ctccctcgcc atgcggggat ttaatcatcg
    16141 gcatcatctc atttccttgt cacagtctta atcgttaacg gaagcccgtt gccgcctgcg
    16201 ctccataacg cgacatccgg gaaatgtctg gattaccagc aaatgtcagg cctggctccg
    16261 ctcaggccga tgaaacaacc ccgcaagggg aattacgcgt ttttcaacat ccactcaatt
    16321 tctgtttctg tgatgaggcg ctcaaactgc aacagctcat catttttaca ggcgtgatac
    16381 acatggcaaa accgttcgcc taaatagcgg cgcaggtgat cattctcgat aaactcaccc
    16441 agggcatcgc tctggcgaat cgggaagggt aagccttcct gttccagccc gttgccttcg
    16501 acttcttcct gcaacggcag ctcgttatca aggccatgca aaataccggc aaaaatcgct
    16561 gccatcacca gatatgggtt ggcatcggca cccgccacgt gatattccac gcggtgatta
    16621 tgacggtcac cgcacggaat acgcagggcg acggtgcggt tgttatggcc ccacgacgcc
    16681 tgcgtcggca catacattcc cggctggaag cggcgatacg agttcacgtt tggtgccagc
    16741 aacgccatcg acgacggcat caggtcaatc atcccggcga gcatcttttt cagcagcggc
    16801 gaatcttcgc cttctgtgtc agaaagcaca ttctcgccac gattattttg catactgata
    16861 tggatatgca tcccgctgcc cgcgtgctct tcatacggct tcgccataaa agtggcgtgc
    16921 atcttatgct tttctgccat cagacgcacc aggcgtttta gtgccagcgc atcatcgcag
    16981 gcttccagca cgttatcagt atggtaaagg ttgatttcaa actgacccgg cgaggcctca
    17041 gcgaccgcgc catctgccgg aatcagctgc aactgcgcca gttcatcaat atcattgagc
    17101 acgtcggcga agtggttgag gttatcaacg gagtaaacct ggctttgtgt attgcggtca
    17161 tcggtgccag gcgcgcaggg cggttgcaga tccccttcag cgtcgcgctg gcgatccagt
    17221 aaatagaact ccagctctac cgctacaacc gggaacaatc cgcgctggcg cagctgctgc
    17281 cacaggcggt taagaacgtt ccgcggctca acgtcaaagg gagcgccatc ttcatcgacc
    17341 atggtcagga gcatctgatc gataaactct ggatcggcgg cagaaggagt taaggaaccg
    17401 agaacaggaa cacaggtacg atccggctcg cccatttcct gacccagacc tgcttcttct
    17461 actacgttgc ccagaatatc cattgcaaat accgaggccg ggaaataaca ccctttctcg
    17521 agcttcttca ggcttgaaac agggatacgt tttccacgga agcaaccatt caaatcagta
    17581 agtaaaacat caacatattg cgtattcggg tagcgctcca ggtagcgttt cacttcctgc
    17641 gtaaaggcgc taccccgcct ctcttctgac tgctgaacaa agttctctac ttcaacgata
    17701 ttggtttcca tgattcttcg cctttggttt gttttccgct cgttatcaaa gcgtaaaata
    17761 taatgaccat cattcgaatc tgtatgcaaa caaaatgttt gtcaaatgtt aaatttagtt
    17821 tgcaaaaatg aaaatccact gctagattga aaaaatattg aacataaagg tcatttaaag
    17881 cgcagtaatg gcgataattt agtccacttt gtgagattga gcatggaaaa tataatgaac
    17941 aatccggtta tcggtgtcgt aatgtgcagg aacaggctta agggtcatgc gacccagact
    18001 ctgcaagaaa agtacctgaa tgccatcatc cacgcaggcg gcttgccaat tgcgctacca
    18061 catgcgctgg cggaaccgtc gttacttgaa caacttttgc cgaaactcga tggcatttat
    18121 cttcctagta gtcccagcaa tgtgcagccg cacctatatg gtgaaaacgg cgatgagcct
    18181 gacgccgatc ccgggcgtga tcttctgagc atggcgataa ttaatgccgc actcgaaagg
    18241 cgcatcccca ttttcgccat ctgccggggt ttacaagaac tggtggtggc aaccggtggt
    18301 tcgttgcatc gcaagctgtg cgaacagcct gaattgctgg agcatcggga agatcccgaa
    18361 ctgccggtgg aacagcaata tgcaccgtcg catgaagttc aggttgaaga ggggggatta
    18421 ctgtctgcgt tgttacctga atgtagcaac ttttgggtaa actctctaca tggacaaggg
    18481 gcgaaggtcg ttagcccacg gttgcgtgtt gaagctcgct cgccggatgg cttagttgag
    18541 gcggttagcg tcatcaatca tccttttgcg ctgggcgtac agtggcaccc ggaatggaac
    18601 agtagcgagt acgcgctttc gcgtatattg ttcgagggct ttatcaccgc ttggcagcac
    18661 catatcgctg aaaaacagcg actctgacca ctacagttta aggaaatgca aatatgagtg
    18721 atgagggact ggcgccagga aaacgcttgt cggaaatccg ccagcagcag gggctttcac
    18781 aacgtcgtgc cgccgaactc tccgggctga ctcacagtgc tatcagtacg atagaacaag
    18841 ataaagtcag ccctgccatc agtacgctgc aaaagctgct gaaggtgtat ggtctgtcac
    18901 tctcggaatt cttttccgag ccggaaaaac ctgatgagcc gcaggtcgtc attaatcagg
    18961 acgacttaat tgagatgggc agtcagggtg tgtcaatgaa gctggttcat aacggtaacc
    19021 cgaatcgcac gctggcgatg atctttgaaa cgtaccagcc gggcacaacc actggggaaa
    19081 gaattaagca tcagggtgag gaaataggca cagtactgga aggtgaaatt gttctgacga
    19141 ttaatggtca ggattaccac ctcgtcgcgg ggcaaagtta tgccattaat accggcatcc
    19201 cgcacagttt cagtaatacg tcggcaggta tttgccgaat tatcagcgcc catacgccca
    19261 ccacgtttta atctttttgt tctgtaagcc gggtaagcgc aatgcgcccg gcaatccata
    19321 aagaatgtcg ccagaaataa acgggcatgc agcccgggga tctctgcgcc ctgacgttca
    19381 caaactgcat atatctgata gacgtgaaac aggagtcata atgaattttc atcatctggc
    19441 ttactggcag gataaagcgt taagtctcgc cattgaaaac cgcttattta ttaacggtga
    19501 atatgctgct gcggcggaaa atgaaacctt tgaaaccgtt gatccggtca cccaggcacc
    19561 gctggcgaaa attgcccgcg gcaagagcgt cgatatcgac cgtgcggtga gcgcagctcg
    19621 cggcgtattt gaacgcggcg actggtcact ctcttctccg gctaaacgta aagcggtact
    19681 gaataaactc gccgatttaa tggaagccca cgccgaagag ctggcactgc tggaaactct
    19741 cgacaccggc aaaccgattc gtcacagtct gcgtgatgat attcccggcg cggcgcgcgc
    19801 cattcgctgg tacgccgaag cgatcgacaa agtgtatggc gaagtggcga ccaccagtag
    19861 ccatgagctg gcgatgatcg tgcgtgaacc ggtcggcgtg attgccgcca tcgtgccgtg
    19921 gaacttcccg ctgttgctga cttgctggaa actcggcccg gcgctggcgg cgggaaacag
    19981 cgtgattcta aaaccgtctg aaaaatcacc gctcagtgcg attcgtctcg cggggctggc
    20041 gaaagaagca ggcttgccgg atggtgtgtt gaacgtggtg acgggttttg gtcatgaagc
    20101 ggggcaggcg ctttcgcgtc ataacgatat cgatgccatc gcctttaccg gctccacgcg
    20161 tacgggtaaa caactgctga aagacgcggg tgatagcaac atgaaacgtg tctggctgga
    20221 agcgggcggc aaaagcgcca acatcgtttt cgccgattgt ccggatctgc aaaaagcggc
    20281 aagcgccacc gccgcaggca tcttctacaa ccagggccag gtgtgcatcg ccggaacgcg
    20341 tttgttactg gaagagagca tcgccgatga attcttagcc ctgttaaaac agcaggcgca
    20401 aaactggcag ccgggccatc cacttgatcc cgcaaccacc atgggcacct taatcgacag
    20461 cgctcacgct gactcggtac acacctttat tcgtgaaggc gaaggcaagg ggcaactgct
    20521 gctggatggt cgcaacgccg ggctggcagc ggccatcggc ccgaccatct tcgtggatgt
    20581 ggacccgaat gcgtccttaa gccgcgaaga gattttcggt ccggtgctgg tggtcacccg
    20641 tttcacatca gaagatcagg cgctgcagct tgccaacgac agccagtacg gccttggcgc
    20701 ggcggtatgg acgcgcgacc tctcccgcgc gcaccgcatg agccgacgcc tgaaagccgg
    20761 ttccgtcttc gtcaataact acaacgacgg cgatatgacc gtgccgtttg gcggctataa
    20821 gcagagcggc aacggtcgcg acaaatccct gcatgccctt gaaaaattca ctgaactgaa
    20881 aaccatctgg ataagcctgg aggcctgaaa tgaccgaaca taccagcagt tactacgccg
    20941 ccagtgcgaa taaatatgca ccattcgaca cactgaatga gtcaatcacc tgcgacgttt
    21001 gcgtggttgg cggcggctat accgggctct cctccgcgct gcatctggcg gaagcgggct
    21061 ttgacgtagt ggttctcgaa gcctcacgca tcggctttgg cgcaagcggg cgcaatggcg
    21121 ggcagcttgt gaactcctac agccgcgaca tcgacgtgat cgaaaaaagc tacggcatgg
    21181 acaccgcccg tatgctcggc agcatgatgt tcgagggtgg cgagatcatc cgcgaacgta
    21241 tcaaacgtta tcagattgac tgcgactacc gccccggcgg tctgtttgtg gcgatgaatg
    21301 ataaacaact cgccacactc gaagagcaga aagagaactg ggaacgctac ggcaataaac
    21361 agctggagct gctggacgcc aacgctattc gccgcgaggt cgccagcgat cgctacactg
    21421 gtgcgctgct ggatcacagc ggtgggcata ttcacccgct aaacctcgcc attggtgaag
    21481 ccgatgccat ccgcctgaac ggcgggcgcg tgtatgagct ttccgccgtg acgcagatcc
    21541 agcacaccac gccagccgtg gttcggactg ccaatggtca ggtgacggcg aagtatgtga
    21601 ttgtcgccgg gaatgcgtat ctgggcgata aagtagagcc ggaactagcg aaacgcagca
    21661 tgccgtgcgg cacgcaggtg atcaccaccg aacggctgtc ggaagattta gcccgttcgc
    21721 tgatcccgaa aaactactgt gtggaagact gtaactatct gcttgattac taccgtctta
    21781 ccgccgacaa ccgcctgctg tacggcgggg gtgtagtgta tggtgcacgc aacccggatg
    21841 acgttgagcg tcttgtggta ccgaaactac taaaaacctt ccctcagttg aagggcgtga
    21901 aaattgatta ccgctggacg ggcaacttcc tgctgacctt gtcgcgcatg ccgcagttcg
    21961 gccgccttga taccaacatc tattacatgc agggttacag cggccacggc gtcacctgta
    22021 cccatcttgc cgggcgtttg attgctgaac tgctgcgcgg tgacgccgag cgtttcgatg
    22081 ccttcgccaa tctgccgcat tacccgttcc ccggcgggcg cacgctgcgc gtgccattta
    22141 ccgcgatggg cgcggcttat tacagcctgc gcgatcgtct gggcgtttaa tttccgatta
    22201 acagtgaaga gtcaaaaggt gtgaaacatg agcaacaatg aattccatca gcgtcgtctt
    22261 tctgctaccc cacgcggggt tggcgtgatg tgtaacttct tcgcccagtc ggcagaaaac
    22321 gctacgctga aggacgtaga gggcaacgaa tacatcgatt tcgccgcagg cattgcggtg
    22381 ctgaataccg ggcatcgcca ccctgatctg gtcgcggcgg tggagcagca attgcaacag
    22441 tttacccaca cggcgtatca gattgtgccg tacgaaagct acgtcacgct ggcggagaaa
    22501 attaacgccc ttgccccagt gagcgggcag gctaaaaccg cattcttcac taccggcgcg
    22561 gaagcggtgg aaaacgcggt gaaaattgct cgcgcccata ccggacgacc tggcgtgatt
    22621 gcgtttagcg gtggcttcca cggtcgtaca tatatgacca tggcgctgac cggaaaagtt
    22681 gcgccgtaca aaatcggctt cggcccgttc cctggttcgg tgtatcacgt accttatccg
    22741 tcagatttac acggcatttc aacacaggac tccctcgacg ccatcgaacg cttgtttaaa
    22801 tcagacatcg aagcgaagca ggtcgcggcc attattttcg aaccggtgca gggcgagggc
    22861 ggtttcaacg ttgcgccaaa agagctggtt gccgccattc gccgcctgtg cgacgagcac
    22921 ggtattgtga tgattgccga tgaagtgcaa agcggctttg cgcgtaccgg taaactgttt
    22981 gccatggatc attacgccga taagccggac ttaatgacga tggcgaaaag cctcgcgggc
    23041 gggatgccgc tttcgggcgt ggtcggtaac gcgaatatta tggacgcgcc cgcgccgggc
    23101 gggttgggtg gcacctacgc cgggaacccg ctggcg
//
LOCUS       DAJTYG010000059        22570 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTYG010000059 DAJTYG010000000
VERSION     DAJTYG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.059"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..1046)
                     /locus_tag="OO568_002479"
     CDS             complement(<1..1046)
                     /locus_tag="OO568_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6641797.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1543..2097
                     /gene="pinE"
                     /locus_tag="OO568_002480"
                     /pseudo
     CDS             1543..2097
                     /gene="pinE"
                     /locus_tag="OO568_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2436..3284)
                     /locus_tag="OO568_002481"
     CDS             complement(2436..3284)
                     /locus_tag="OO568_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6641798.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3493..4128
                     /gene="yfhb"
                     /locus_tag="OO568_002482"
     CDS             3493..4128
                     /gene="yfhb"
                     /locus_tag="OO568_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6641799.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4153..4689
                     /gene="tadA"
                     /locus_tag="OO568_002483"
     CDS             4153..4689
                     /gene="tadA"
                     /locus_tag="OO568_002483"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6641800.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(4686..6242)
                     /gene="mltF"
                     /locus_tag="OO568_002484"
     CDS             complement(4686..6242)
                     /gene="mltF"
                     /locus_tag="OO568_002484"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6641801.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6500..10387
                     /gene="purL"
                     /locus_tag="OO568_002485"
                     /gene_synonym="purI"
     CDS             6500..10387
                     /gene="purL"
                     /locus_tag="OO568_002485"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6641802.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10945..12372
                     /gene="qseE"
                     /locus_tag="OO568_002486"
     CDS             10945..12372
                     /gene="qseE"
                     /locus_tag="OO568_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6641803.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRIIWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12537..13250
                     /gene="qseG"
                     /locus_tag="OO568_002487"
     CDS             12537..13250
                     /gene="qseG"
                     /locus_tag="OO568_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6641804.1"
                     /translation="MRHIFQRLLPRRPWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13240..14574
                     /gene="glrR"
                     /locus_tag="OO568_002488"
     CDS             13240..14574
                     /gene="glrR"
                     /locus_tag="OO568_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6641805.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14635..14973
                     /gene="glnB"
                     /locus_tag="OO568_002489"
     CDS             14635..14973
                     /gene="glnB"
                     /locus_tag="OO568_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6641806.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15018..16208)
                     /gene="hmpA"
                     /locus_tag="OO568_002490"
     CDS             complement(15018..16208)
                     /gene="hmpA"
                     /locus_tag="OO568_002490"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6641807.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16535..17788
                     /gene="glyA"
                     /locus_tag="OO568_002491"
     CDS             16535..17788
                     /gene="glyA"
                     /locus_tag="OO568_002491"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6641808.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDAAGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(17986..19179)
                     /locus_tag="OO568_002492"
     CDS             complement(17986..19179)
                     /locus_tag="OO568_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR6641809.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLAQAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKTTQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            19297..19470
                     /locus_tag="OO568_002493"
     CDS             19297..19470
                     /locus_tag="OO568_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641810.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            19477..>22570
                     /locus_tag="OO568_002494"
     CDS             19477..>22570
                     /locus_tag="OO568_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR6641811.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLCMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     Y"
BASE COUNT         5398 a         5613 c         6008 g         5551 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccccc
     1501 cccgactttt gacgcagtgt cttgttaaca gggggattta ccatgcttat tggctatgta
     1561 cgcgtatcaa caaatgacca gaataccgat ctacaacgta atgcgttgaa ctgtgcagga
     1621 tgcgagctga tttttgaaga caagataagt ggtacaaagt ccgaaagacc ggggctgaaa
     1681 aaactgctca ggacattatc ggcaggcgac acactggttg tctggaagct ggatcggctg
     1741 gggcgcagta tgcggcatct tgtcgtgctg gtggaggagt tgcgcgaacg aggcatcaac
     1801 tttcgtagtc tgactgattc aattgatacc agcacaccaa tgggacgctt tttcttccat
     1861 gtgatgggtg ccctggctga aatggaacgt gaactgattg ttgaacgaac aaaagcagga
     1921 ctggaagctg ctcgcgcaca gggacgaatt ggcggtcgcc gtcccaaact ttcaccagaa
     1981 taatgggcgc aggccgggcg actaattgca tcaggagttt ctcgccagaa ggtggcgatc
     2041 atctatgatg ttggcgaatc gactttgtat aagaggtttc cagccagggt tatataaaac
     2101 cgcagacacg gcgtatacaa ggacgtgcta cggttgactg aaaagttaat gttgtatgtc
     2161 acaggccttt tgcgccgtaa agccccgtcc tttagggcgc ggaggacgtt aaagaaagtg
     2221 gtgttctttg tgttgctgaa cttatgcaaa atttttttgc accataaatc caacaacaca
     2281 ttcaataaca cattgctgta tcactatgaa aataaatgtg tttttatttg atttcacaca
     2341 tatcacaatt gatgcatttt ttagtgatta acagcgccat aaggaaactc tcaaaacaac
     2401 acaaaccggg cgggcattat acgcgcattc tttactcaga ccagtttttt caccagcgct
     2461 tcactatgac gaatacgttc tggtgccagt tccagatcct gctgaatcag cgcaatgaac
     2521 agcaaatccg ttaacattcc ctgagcgtga caagcagaga ttgaagcact gtttgtcgct
     2581 tgttcttcgg caatggtata caggcaatga gaagcacgct gttgcagggc attcggagta
     2641 aagccggtaa tcgccagcac ttttccgccc actcgcagca tctcatctgc cgccaggttt
     2701 aactcgcgtc gtacaccggt gtaggaaatg gctaataaca gatcgtcagg ggacgacgcc
     2761 tgtactgttg cgagtagcgc atgcatatcg cgcagtgcgg cagcattgaa gccaatcttc
     2821 atcagcttcc aggcaaagtt ttgcgccacc agacccgaag cgccaatacc ggtcagaatt
     2881 atccgccgcg cagagcgcaa cattgttacg cattcatgca gtttctcttc actattaacg
     2941 tttagcgttg cgtacatagc ggcagtattt tctttaatca gtttttcgcc gaccagccgt
     3001 aacggatcat caccgcggat ttggttatga atgggcacgg agggcgattc cggctggctt
     3061 gccagcgctt cactcaacgc caacttaagc gccggaaaac ctttatagcc gagtttttgc
     3121 gcgaacttca cgacgctgga ctgactgact ccggcttcgt tggccagttg ctgagagctt
     3181 aaatggcgcg ccgtatcagg ttgtagcagc agataatccg ccagtttttt atcactctgg
     3241 gcaagcccct ggtaacgctg acggatacga agtaagccgt tcatatcttt cctcgcgatg
     3301 aaatagtggc gacaacaggc aatatgagac acacgcgagt gaattttata ttccattgcc
     3361 gctaattgcc atgaattaaa aattccagtt agccatgatg cggtcgattg ttgccggaaa
     3421 agtgatgatc tatctgttag gctatgtagt agcgaaaatt aatcaggatg tttcagtcca
     3481 gaggagtatg gtttggcaac tcacgagcgt cgtgtggtgt tttttgactt agatggaaca
     3541 ttgcatcagc aggatatgtt cggcagtttt ctgcgctatt tactgcgtcg ccaaccgctg
     3601 aatgcgttac ttgtcctgcc gttgttaccg attatagcca ttgcgttatt gataaaaggt
     3661 cgtgcggcac gctggccgat gagtctgctt ctgtgggggt gcacttttgg tcacagcgaa
     3721 gcacgtttac agacgttgca ggccgatttc gtgcgctggt ttcgcgacaa tgttaccgcc
     3781 tttccgctgg ttcaggagcg attaaccacc tacctgttaa gttccgatgc tgatatctgg
     3841 ttgattaccg gctctccgca gccgctggtt gaagcggttt atttcgatac gccctggctg
     3901 ccgcgggtta atcttatcgc cagccaaatt cagcgtggct atggtggttg ggtattgacg
     3961 atgcgttgtc tgggacatga aaaggtcgca caactggagc gcaaaatcgg cacgccgctg
     4021 cggctgtaca gtggctatag cgacagtaat caggacaatc cgctgcttta tttctgtcag
     4081 catcgttggc gagtaacccc gcgcggtgaa ctccagcaac tggaatagag taaagcatag
     4141 cgtccgtgta taatgcgccg cgcttttata accggagttt tctttttgtc tgaagtcgaa
     4201 tttagccacg aatactggat gcgtcacgcg ctgacgctgg cgaaacgtgc ctgggatgag
     4261 cgggaagtgc cggtcggcgc ggtattagtg cataacaatc gggtaatcgg cgaaggctgg
     4321 aaccgcccga ttggtcgcca tgatcccacc gcacatgcag aaatcatggc cctgcggcag
     4381 ggtggtctgg tgatgcaaaa ttatcgtctg atcgacgcca cgttgtatgt cacgcttgaa
     4441 ccatgtgtaa tgtgtgccgg agcgatgatc cacagtcgca ttggtcgcgt ggtctttggt
     4501 gcgcgtgacg cgaaaactgg cgctgcggga tctttaatgg atgtgctgca tcatccgggt
     4561 atgaatcacc gagtggaaat tacggaagga atactggcgg atgagtgcgc ggcgttgctc
     4621 agtgacttct ttcgcatgcg ccgccaggaa attaaagcgc agaaaaaagc gcaatcctcg
     4681 acggattaat tttgtttctc ttcactccct ttcctggaaa acagcagaga gggagaatgg
     4741 gtcaaatagt ttgatgacga ctgggaaaga aacgagagaa aaggaaattt ctctttgccc
     4801 aactccgtag gcgataccgc cggataatcc tgcgccagtt gcatcgccgc ttctgtagcc
     4861 tgcttctctt tctcttgcag ataacccacc aggctaatct gatacttacg aatattttcg
     4921 acataagcgt aggcttcatg cccacgagcg tagccgtaag tcagcttgct gtaatagggt
     4981 ttctggctaa gtaaaggcag acgctgtttt acgtcagccc aactgtcagg attccctttg
     5041 gtttttgccg tcagggcgcg ggcatccagc atatgcgcat agcccatatt gtacgcagcg
     5101 agggcaaacc agatccgttc gttctccggc acactttccg gcactttact catcatatcc
     5161 tgcaaataac gcacgccacc gctgatgctc tgttcggcat cggtacgatc cgtaatgccg
     5221 aggctttgcg cggtattttt ggttaacatc atcatgccgc gcacacccgt cggtgaagtg
     5281 gcctgtgcat cccagtgcga ttcctgataa gcaatagcgg ccagcaaacg ccagtcaatt
     5341 tcttcggcgt atttctcaaa caggggcttt aactgcggca gtaccgcatc gacggcgcgt
     5401 aaaaatgtgc gcgtatcgac gtaatcaaaa tcatcgccat gccccaggta tttctcttca
     5461 atgcgtgcca gcgtaccgtc ttcattcatt tcgttgaaga agtcgagcag ggcggcggaa
     5521 agggtattat cgccatctaa cgggctaaac caagtcaccg gttgttcatc ggtgatatcg
     5581 agcgctacgg cgagctccgg gtgaacgcgc tgaaacaggc tgatggcgac agaatcagca
     5641 atggtgtaat cgagttttcc ttcgatgaca tcttccatta attccgcaga gccttttttg
     5701 tcgtctacct tccagcttaa ttccgggaat tttgtttctt tcagggtctg gagatcgtta
     5761 accaccacat gacccggtgc aacggtgagt tgctccgccg tcaggttgcc cagcgtacgt
     5821 gggcgatact gacccacttt ataaaccagt tgttgtgaca cggaataata ggtagggcca
     5881 ggctgataat tttttacccg ctcactgtta tagaccagtc ctgccgccag caggtcggcg
     5941 ttaccattat caaggtcgtc aaacagctgg ctgatattct gccgcacggt cactttcagt
     6001 tttacgccga ggtaatcggc aaactgtttc gccagttcgt aatccaggcc aaaaggtttc
     6061 ccgttgattt cgttataagt caggggagta tgaatggtgc tcacacgcaa ctctccccgc
     6121 gcttgaatgg cggcgatacg gttgtcggct ttaccaaacc agggaatgga tggccagaga
     6181 gcgaccgcga gcagcagtgc cagaatgccg atgaacagat aattaatctt taattttttc
     6241 aattagttaa ttctctgtgt cgtgcgcgtc ccagcttgaa aaaacgtaat aatagtgaaa
     6301 ggtttactca taaatgagcg gcattttgcg taaacctgcg ccagatggca acttattaca
     6361 gccattggcg gcacgcgttg ctaattcacg atggtgattt tatttccacg caaacggttt
     6421 cgtcagcgca tcagattctt tataatgacg cccgtttccc ccccttgggt acaccgaaag
     6481 cttagaagac gagagactta tgatggaaat tctgcgtggt tcgcctgcac tgtcggcatt
     6541 ccgaatcaac aaactgctgg cacgttttca ggctgccagg ctcccggttc acaatattta
     6601 cgccgagtat gtccattttg ctgacctcaa tgcgccgtta aacgatgatg agcacgcaca
     6661 acttgaacgc ctgctgaaat atggtccggc actcgccagc cacgccccgc aaggcaaact
     6721 cctgctggtg accccgcgtc ctggcaccat ctctccctgg tcttcgaaag cgaccgatat
     6781 tgcccataac tgcgggctac aacaggtaaa ccgccttgag cgcggcgttg cttactatat
     6841 agaagccggt acgctgacca atgaacaatg gcagcaggtt accgctgaac tgcacgaccg
     6901 catgatggaa acggtctttt ttgatttaga tgatgcagag cagttgtttg cccaccatca
     6961 accgactccg gtcacaagcg ttgatttgct ggggcagggc cgtcaggcgc tgatcgacgc
     7021 taacctgcgt cttggcctgg ctctggcgga agatgaaatt gactatctgc aggatgcttt
     7081 cacaaagctt ggtcgtaacc cgaacgacat cgagctgtat atgttcgctc aggcgaactc
     7141 tgagcactgc cgccacaaaa tttttaacgc cgactggatt atcgacggtg aacaacagcc
     7201 gaaatcgctg ttcaggatga tcaaaaatac tttcgaaacc acgccagatc acgttctctc
     7261 tgcttataaa gataacgccg ccgtaatgga aggttctgaa gtgggccgct actttgctga
     7321 ccacgaaacg ggccgctaca atttccatca ggaaccggcg cacatcctga tgaaagtcga
     7381 aactcacaac cacccgacgg cgatttctcc gtggccgggc gcggcgaccg gttccggcgg
     7441 tgaaatccgc gatgaaggtg ccaccgggcg cggcgcgaag ccgaaagctg gtttggttgg
     7501 tttctccgta tccaacctgc gaattcctgg cttcgaacag ccgtgggaag aagatttcgg
     7561 taagcctgag cgcattgtta ccgcgctgga catcatgacc gaaggcccgc tgggcggtgc
     7621 ggcgtttaac aacgaatttg gtcgtccggc actgaacggc tacttccgta cttatgaaga
     7681 aaaagtgaac agccacaatg gcgaagagct gcgcggttat cacaaaccga tcatgctggc
     7741 gggcgggatc ggcaacattc gcgccgatca cgtacaaaaa ggcgagatca acgtcggtgc
     7801 gaagctggtc gttctcggcg gcccggcaat gaacatcggt cttggcggcg gcgcggcgtc
     7861 ttccatggcg tctggtcagt ctgatgccga cctcgacttt gcttccgtac agcgcgacaa
     7921 cccggagatg gagcgtcgtt gccaggaagt gatcgaccgt tgctggcagc ttggtgatgc
     7981 taacccaatt ctgtttatcc acgacgtcgg tgcgggcggt ctttctaacg cgatgccgga
     8041 gctggtgagc gacggcggac gcggcggtaa atttgaactg cgcgatattc taagcgacga
     8101 accgggcatg agcccgctgg aaatctggtg taacgaatcc caggaacgct acgtgctggc
     8161 ggttgctgcc gatcaattac cgctgtttga cgaactgtgt aagcgtgagc gcgcacccta
     8221 cgcggtgatt ggtgaagcga ccgaagaact gcatctttct ctgcacgatc gtcattttga
     8281 taatcagccg atcgatctgc cgctggacgt cctgcttggt aaaacgccga agatgacccg
     8341 cgatgtacaa acgctgaaag cgaagggcga tgcgctggtc cgtgaaggga tcaccattgc
     8401 agacgcggtg aaacgcgtac tgcatctgcc gaccgtggcg gagaaaactt tcctggtgac
     8461 catcggtgac cgcagcgtaa ccggcatggt ggcgcgcgat cagatggtgg ggccgtggca
     8521 ggtgccggtc gctaactgcg cggtcactac cgccagcctc gacagctact acggtgaagc
     8581 gatggcgatt ggcgagcgtg cgccggttgc gctgctggat ttcgccgcct ctgcccgtct
     8641 ggcggtcggt gaagcgttaa ccaatattgc cgcaacacag attggcgata tcaaacgcat
     8701 taaactttcc gccaactgga tggcggcggc aggccaccct ggtgaagatg cgggcctgta
     8761 tgaagccgtt aaagccgtgg gtgaagaact ttgtccggcg ctgggcctga cgatcccggt
     8821 gggtaaagac tctatgtcga tgaaaacccg ctggcaggaa ggtaacgaag agcgcgaaat
     8881 gacgtcgccg ctgtcgctgg tgatttctgc atttgcccgc gtggaagatg tacgtcacac
     8941 catcacgccg cagctttgta ccgaagataa cgcactgctg ctgattgatt tgggcaaagg
     9001 caataacgcg ctgggcgcaa cggcgctggc gcaggtttat cgtcagcttg gcgacaaacc
     9061 ggcagatgta cgcgatgttg tccaactgaa aggcttctat gacgcgattc aggcgctggt
     9121 tgcacagcgt aagctgctgg cgtatcacga ccgctctgat ggcggcctgc tggtaacgct
     9181 ggcggaaatg gcctttgcag gtcattgtgg cattgacgcg gatatcgcct ctctgggtga
     9241 cgatcgcctg gcggcgttgt ttaacgaaga actgggtgcg gtgattcagg ttcgtgccgc
     9301 tgaccgtgaa gcggtcgagt ccgtactggc acagcatggg cttgcggatt gcgtccatta
     9361 tgttgggcag gcggtttccg gtgaccgttt tgtgattacc gccaacgggc agactgtatt
     9421 cagcgaaagc cgcacaacgt tgcgtgtctg gtgggcagaa actacctggc agatgcagcg
     9481 cctgcgtgac aacccggagt gtgccgatca ggagcatcag gcgaaatcta acgacgccga
     9541 tccgggcctg aatgtaaaac tgtcgttcga tatcaacgaa gatgtggcag caccgtatat
     9601 tgccactggc gcacgtccga aagttgccgt actgcgtgag cagggcgtga actcgcatgt
     9661 tgaaatggcg gcagctttcc aacgtggggg ctttgatgct atcgacgtgc atatgagcga
     9721 cctgctgact ggacgtacgg gactggagga ttttcacgcc ctggtcgcgt gcggaggttt
     9781 ctcctacggt gatgtgctgg gtgccggtga aggttgggcg aagtctatcc tgttcaatga
     9841 ccgtgtacgc gatgagtttg caaccttctt ccatcgtccg caaacgctgg ccctgggggt
     9901 atgtaacggt tgccagatga tgtctaacct gcgtgaactg atcccgggga gcgagttgtg
     9961 gccacgtttt gtgcgtaaca cctccgatcg ctttgaagcg cgtttcagcc tggttgaagt
    10021 aacccaaagc ccgtctctgc tgttgcaggg gatggtgggc tcgcaaatgc cgattgctgt
    10081 ctctcatggt gaagggcgcg tggaagtgcg tgatgcggcg catctggcgg cactggaaag
    10141 caaagggctg gtggcactgc gctatgtcga taacttcggc aaagtcactg aaacctaccc
    10201 ggctaacccg aacggttcac cgaacggtat tacggcggtt acgactgaaa gtggtcgagt
    10261 caccattatg atgccgcacc cggaacgtgt tttccgtact gtcagcaact cctggcatcc
    10321 ggaaaactgg ggcgaggatg gcccatggat gcgcattttc cgcaatgcgc gtaagcagtt
    10381 ggggtaagtc atcagttcat tggttttgca gcccggatgc ggcattaagc accaaccact
    10441 atcataagct cccaactccg ggagcttttt tgtgtctgta aatcacgaca atgggtggtt
    10501 taccgtgtcg ctttctggcg acacttaaat gattgatttt attgtaatct aataagttta
    10561 tcttaagttg tctcttttta gcgacacagt ggctgattta cattcaaata gcgaagaagc
    10621 cataaatctc tcaaagcaac gtataatcag tacgttacca aactattttc tttattggca
    10681 cagttactgc ataatagtaa gcagtggctc attcaccgac ttatgtcagc cccttcggga
    10741 cgtgctacat aaaattcgaa tgacgcacaa caaggtgcct gccgtccaac ttctgatatc
    10801 agcgtagcta tatcaaccat cgggcgaaac gtcgagttag gcaccgcctt attccataac
    10861 aaagccgggt aattcccggc tttgttgtat ctgaacttcc cctcggttag catcaggcta
    10921 ttcgcgtctg acgagagtaa caccttgaaa cgctggcccg tttttccccg ctcattacga
    10981 caactggtaa tgctggcatt tttgctaatt ctactacccc tgttggtgct ggcatggcaa
    11041 gcctggcaaa gcctgaatgc gcttagcgat caggcggcgc tggttaaccg cactacgctt
    11101 attgatgccc ggcgtagtga agcgatgacc aacgcggcgc tggagatgga gcgtagctac
    11161 cgtcagtatt gcgtgctgga cgaccctacg cttgcgaagg tttatcaaag ccagcgcaag
    11221 cgttacagcg aaatgctcga tgcccacgca ggcgtactgc cagacgataa actttaccag
    11281 gcattgcgtc aggatttgaa caatctggcc caacttcagt gtaacaacag tggtcccgat
    11341 gctgccgccg ccgcgcgtct tgaagccttt gccagtgcca ataccgaaat ggtgcaggcc
    11401 acgcgcacag tggtgttctc tcgtgggcag caacttcagc gtgaaatcgc cgaacgtggg
    11461 caatattttg gttggcaatc gctggtgcta tttctggtga gtctggtgat ggtgctactt
    11521 ttcacgcgga tgattatcgg gccggtgaaa aatatcgagc gcatgatcaa ccgtctgggg
    11581 gaagggcgtt ctctgggcaa tagcgtttcg ttctgtggac cgagcgagtt acgctcggtt
    11641 gggcaacgta ttatttggtt aagtgagcgc ctgtcatggc tggaatccca acgccatcaa
    11701 tttttaagac atttatctca tgaattaaaa acgccactgg cgagtatgcg cgagggcact
    11761 gaattactgg ctgatcaggt tgtcgggccg cttacgccag agcaaaaaga ggtggtgagc
    11821 attcttgata gcagcagctg caatttgcaa aaactgatcg aacaactgct tgattacaac
    11881 cgtaaacagg cggacagtgc ggtggaactg gagaatgttg aattagcacc gctggtggag
    11941 acagtggttt ctgctcatag cctgcccgca cgggctaaaa tgatgcatac cgacgtcgat
    12001 ctcaaagcaa cagcttgcct ggcggagcca atgctgctga tgagcgtact ggataatctt
    12061 tactccaatg cggtgcacta cggggctgaa tccggtaaca tttgccttcg cagcagttta
    12121 catggtgcgc gggtttatat tgatgtcatc aatacaggca cgcccattcc gcaagaggaa
    12181 cgcgccatga tcttcgaacc cttttttcag ggaagccacc agcgaaaagg ggcggtgaag
    12241 ggcagcggtc tgggattaag cattgccagg gattgtattc gccgtatgca aggggaactg
    12301 tatctggtcg acgagagcgg gcaagacgtt tgtttccgca ttgaattacc gtcgtcgaaa
    12361 aacacgaaat aatgctaaca gcctatccta tcaggctatt ttatttgtca gttcggcccc
    12421 gggtagtgct cgaaatcttc acgtacgata tgtacgcccc ggtttctccg cgctgtccat
    12481 gtccagactg tctgcaacaa ttacgcccac tgggataggc tttaagtctg gtgaatatgc
    12541 gacacatttt tcaacgatta ttgccccgac ggccgtggct ggcagggctg ccatgtctgg
    12601 cattgctggg ttgcgtgcag aatcacaata agccagccat tgatacgcca gcagaagaaa
    12661 aaattccggt ttatcaactg gctgactatc tttctactga atgtagtgac atctgggcgc
    12721 tgcaaggtaa atcaaccgaa accaatccgc tttactggct gcgggcgatg gattgtgctg
    12781 atcgtttaat gcctgcgcag tcgcgtcagc aagctcgcca atatgacgac ggcagctggc
    12841 aaaatacctt taaacaaggg attttgctcg ccgacgccaa aattacgccg tatgagcgcc
    12901 gccagctggt cgcgcgtatt gaagcgttaa gtaccgagat cccggcacag gttcgcccac
    12961 tgtatcaact ctggcgcgac ggtcaggcgc tgcaactgca actggcggaa gagcgccaac
    13021 gttacagtaa actccagcag tccagcgaca gcgagctgga tacattgcgc cagcaacatc
    13081 acgttctaca gcagcaactg gaactcacca cccgcaagct ggaaaacctg accgatattg
    13141 aacgccagct ctcgacccgc aaaccggctg gaaatttctc gccggatacg ccgcacgaga
    13201 gtgaaaagcc cgcgccatcc acccatgagg tcactcctga tgagccataa acctgcgcat
    13261 ttattattgg tcgatgacga tccgggattg ctgaaactgc ttggcctgcg cctgaccagc
    13321 gaaggctaca gtgtggtcac ggcggaaagt ggcgctgaag gattacgggt actgaatcgc
    13381 gaaaaagtag atttagttat cagcgacctg cggatggatg aaatggacgg tatgcagcta
    13441 tttgctgaaa tccagaaagt gcagccggga atgccagtaa ttattcttac tgcgcatggt
    13501 tctatccccg atgccgttgc tgcaacacag cagggcgttt ttagtttcct caccaagcct
    13561 gtcgataaag atgcgctata tcaggcaatt gacgatgcgc tggagcaatc cgcgccagcc
    13621 accgatgaac gctggcgcga ggcaattgtc acccgcagcc cgctgatgct gcgtttgctg
    13681 gaacaggcgc ggctggtggc gcaatcagac gtcagcgttt tgattaacgg tcagagcggc
    13741 accgggaaag agattttcgc ccaggctatc cacaacgcca gcccgcgcaa cagcaaacca
    13801 tttattgcta ttaactgtgg cgcattgccc gagcaattgc tggagtcgga gctgtttggt
    13861 catgcgcgtg gcgcgtttac tggcgctgtc agcaatcgcg aaggtttatt ccaggcggcg
    13921 gaaggcggaa cgctatttct cgatgaaatt ggcgatatgc ctgcgccgtt acaggtcaaa
    13981 ctgctgcgcg tgttgcagga gcgtaaagtg cgcccgctgg gcagtaaccg cgatattgat
    14041 atcaatgtgc ggattatttc tgccactcac cgtgatctgt caaaagcgat ggcgcgcggg
    14101 gaattccgcg aagatctcta ttaccgcctc aacgttgtca gcctgaaaat tccggcactg
    14161 gcggagcgca cagaagacat tccgctactg gcaaatcacc tgttgcgcca ggcggcagag
    14221 cgacataaac cgtttgtccg cgcgttctct accgatgcga tgaaacgcct gatgaccgcg
    14281 agctggccgg gtaatgtgcg ccagttggtc aacgtgattg aacagtgcgt ggcgctgacc
    14341 tcatctccgg tgattagtga tgcgctggtg gagcaggcgc tggagggtga aaatacggcg
    14401 ctgccaacct ttgttgaggc acgtaatcat tttgaactca actatttgcg taagctgctg
    14461 caaatcacca aaggcaacgt cacccacgcg gcgagaatgg cggggcgcaa ccggacagaa
    14521 ttttataaac tgctttcccg acacgagctg gatgcaaacg atttcaagga atgaattggc
    14581 gttatgtgtt acgtttagca gatcaaaaga caggcaacct tttcaaggaa tagcatgaaa
    14641 aagattgatg cgattataaa acccttcaag ctggacgatg tccgcgaagc actggccgaa
    14701 gtcggtatta ccggcatgac ggtgaccgaa gtgaaaggct ttggtcgcca gaaaggccat
    14761 accgagctgt accgcggcgc ggagtatatg gtggattttc tgccgaaagt gaaaattgag
    14821 attgtcgtac cggacgacat tgtcgatacc tgtgtcgata ccattattcg cacggcgcaa
    14881 accggcaaaa tcggtgacgg taaaatcttc gtctttgacg tggctcgggt cattcgcatc
    14941 cgtaccggtg aagaggacga cgcggcaatt taaaccgcgt caagggttgc cggatgtttc
    15001 catccggcaa catcaaatta cagcacctta tgcgggccaa agcattcgta atgaatgttt
    15061 tcctgcttca cgcccagatc cactaactgt ttcgcggtaa actgcatgaa gccaaccggg
    15121 ccgcagagat agaactgcat tgtcggatcg ctgaacgcac cttccagttt gctcaaatcc
    15181 atcagacctt cgctatcaaa ctgaccttta gcgcgatcgg cttcgctcgg ctgacgatac
    15241 caggtgtgcg cggtaaagcg cggcagtgac tgccccagtt ccttaacttc atcagcaaag
    15301 gcgtgaactt cgccattttc tgccgcatgg aaccagttca cttgtgctgt gtggcctgct
    15361 tttgccagcg tgtcgagcat tgccagcatt ggcgtttgac caacaccggt agagattaac
    15421 gtcactggtg tgtcatctgc gacagccata aagaaatcac ctgctggagc gaccagtttc
    15481 acgacatcgc caacattggc gtgattgtgc aaccagttgg atacctgccc accctcttcg
    15541 cgtttcaccg cgatacgata gcctttgcca tccggtttgc gagtcaaaga gtactgacga
    15601 atttcctgat gcgggaaacc ttccggcttc agccagacgc cgagatattg ccccggacgg
    15661 tattctgcca ctgcgccacc gtcgaccggc tccagttcga agctggtgat aagcgcgctg
    15721 cgcggtgttt tagccacaat gcggaaatcg cgagtacctt cccaaccacc ggctttgctg
    15781 gcgttttcgt tatagatttc cgcctcgcga ttgataaata cattagccag tacaccatag
    15841 gctttacccc acgcgtccag cacttcctgc cccgggctga acatttcgtc cagcgttgcc
    15901 aacaggtgtt caccgacgat gttgtactgt tccggtttga tctggaagct ggtgtgcttc
    15961 tgcgcgattt tttctaccgc tggcagcagc gcaggcaggt tttcaatatt actggcgtag
    16021 gcggcaatag cgttaaacag ggcttcacgt tgatcgccat tacgctggtt actcatgtta
    16081 aaaatttctt tgagttctgg gttatgagta aacatacggt cgtagaaatg ggcggttaac
    16141 tttggccccg tttccaccag taaagggatg gtggctttta ctgtagcgat ggtttgagcg
    16201 tcaagcatat ggtcttcctt ttttgcatct taattgatgt atctcaaatg catcttataa
    16261 aaaatagccc tgcaatgtaa atggttcttt ggtgtttttc agaaagaatg tgatgaagtg
    16321 aaaaatttgc atcacaaacc tgaaaagaaa tccgtttccg gttgcaagct ctttattctc
    16381 caaagccttg cgtagcctta aggtaatcgt ttgcgtaaat tcctttgtca agacctgtta
    16441 tcgcacaatg attcggttat actgttcgcc gttgtccaac aggaccgcct ataaaggcca
    16501 aaaattttat tgttagctga gtcaggagat gcggatgtta aagcgtgaaa tgaacattgc
    16561 cgattatgat gccgaactgt ggcaggctat ggagcaggaa aaagtacgtc aggaagagca
    16621 catcgaactg atcgcctccg aaaactacac cagcccgcgc gtaatgcagg cgcagggttc
    16681 tcagctgacc aacaaatatg ctgaaggtta tccgggcaaa cgctactacg gcggttgcga
    16741 gtatgttgat atcgttgaac aactggcgat cgatcgtgcg aaagaactgt tcggcgctga
    16801 ctacgctaac gtccagccgc actccggctc ccaggctaac tttgcggtct acaccgcgct
    16861 gctggaacca ggtgataccg ttctgggtat gaacctggcg catggcggtc acctgactca
    16921 cggttctccg gttaacttct ccggtaaact gtacaacatc gttccttacg gtatcgatgc
    16981 tgccggtcat atcgattacg ccgatctgga aaaacaagcc aaagaacaca agccgaaaat
    17041 gattatcggt ggtttctctg catattccgg cgtggttgac tgggcgaaaa tgcgtgaaat
    17101 cgctgacagc atcggtgctt atctgttcgt tgatatggcg cacgttgcgg gcctggttgc
    17161 tgctggcgtc tacccgaacc cggttcctca tgctcacgtt gttactacca ccactcacaa
    17221 aaccctggcg ggtccgcgcg gcggcctgat cctggcgaaa ggtggtagcg aagagctgta
    17281 caaaaaactg aactctgccg ttttccctgg tggtcagggc ggtccgttga tgcacgtaat
    17341 cgccggtaaa gcggttgctc tgaaagaagc gatggagcct gagttcaaaa cttaccagca
    17401 gcaggtcgcg aaaaacgcta aagcgatggt agaagtgttc ctcgagcgcg gctacaaagt
    17461 ggtttccggc ggcactgata accacctgtt cctggttgat ctggttgata aaaacctgac
    17521 cggtaaagaa gcagacgccg ctctgggccg tgctaacatc accgtcaaca aaaacagcgt
    17581 accgaacgat ccgaagagcc cgtttgtgac ctccggtatt cgtgtaggta ctccggcgat
    17641 tacccgtcgc ggctttaaag aagctgaagc gaaagaactg gctggctgga tgtgtgacgt
    17701 gctggacagc atcaatgatg aagccgttat cgagcgcatc aaaggtaaag ttctcgacat
    17761 ctgcgcacgt tacccggttt acgcataagc gaaacggtga tttgctgaca atgtgctcgt
    17821 tgttcatgtt ggatgcggca tgaacacctt ctcaggccta caaaatcttg taaattcaat
    17881 atattgcaat ctccgtgtag gcctgataag cgtagcgcat caggcaattt ttcgtttatg
    17941 atcatcaagg cttccttcgg gaagcctttc tacgttatcg cgccatcaaa tctgtcgtaa
    18001 ctgcgcctca acatacaaat agccaattcc cagcacctgt tgtgcgcggc ttaattgccc
    18061 gaagccaatt tgcgtcgttt tcacgctcgg tccttcgtcg cggtcgaacg ggttaaatcc
    18121 tgtctggcga ataatagtat taagccagtt ctcaccaaag gcacaactgc gaccgtacaa
    18181 ccaaatctga ttaatattga ggatgttgag gaagttatac agactcaaac caatggcatt
    18241 ggcagagcga tcaacccagc tggtgatcca cggttctcca ctctgccagg cagcgattaa
    18301 ctgcgcggtt gtcagttttt caggatcaag ctgagtatta accggttgtg attttagcca
    18361 tacccgcgcc tgttttttta atgcacttaa cgaggcgacg gtttccaggc agccgtaacg
    18421 tccacagtcg cagacgacgc catccggatt aacgatggtg tgaccaatct gtccgctacc
    18481 atacaaactg ccgcgataaa tttgctcgtt aatcacgaac gacgagccaa tgccgtaatc
    18541 aacgttgatc acgcagaaat cccgttcctg cgaattattt tgccatttct ccgcctgcgc
    18601 cagcatcacg cagtcattat cgaccagcac ccgaatgccc agcttctctt ccagcagata
    18661 cttcacctca actggcgttg tccacggcgc ttgcggcatg gtttgcgata cgccggtcac
    18721 cggatcaacc tgcccgtgga ttgccagcgc cagattgatg gtgcggtccg gccacaattt
    18781 acggtggcga tgccagcatt tttcgatttc ggacagcagc gcctgcggcg tcggtacatc
    18841 aatctgtaaa tactcaaatt cgcctttcgg acttaaacaa gcattagcaa cctgacactc
    18901 aatactggtg ggcgtcacgt tcaggcacag cgtccagtca ccttccggcg caatcagcca
    18961 tgtaccgcta ctatgcccgc gcgtctggct ttcatcgtca atattcacca cccgtttttc
    19021 gctttccagt tcctgcaaaa tattactgac tgccggaatc gaaatttgcg ccagccgggc
    19081 cagcgttgat ttattggcgc gcttttgccg gtacagcagt tccagaatca cgcatttgtt
    19141 atggtggcga atctgttgat tgttaatgca ggctctcatc ttcactaaac cttgtttagt
    19201 taattgcgtg attattccgc gaaaacattg ccagattgtt caatacactg ccacaaatct
    19261 tttaattcag tatgtcttgt taatattgag ggcaccatga ctccagtaaa agtgtggcaa
    19321 gagcgcgttg agatcccgac ctatgaaacc gggccgcagg atatacatcc catgttcctg
    19381 gaaaatcgcg tttatcaggg atcgtccggc gcggtttatc cctacggcgt gaccgatacg
    19441 ctgagcgagc agaaaaccct gaaatcctag caggcggtgt ggctggaaaa cgactacatc
    19501 aaagtgatga tcctgccgga actgggcggt cgggtgcatc gcgcatggga taaagtgaaa
    19561 cagcgcgatt ttgtctatca caatgaagtc attaaacctg cgctggtggg gctgctggga
    19621 ccgtggatct ccggcgggat tgagtttaac tggccgcaac accatcgccc gaccaccttt
    19681 atgcccgttg atttcaccct cgaagcccat gaagacggtg cacagacggt gtgggtcggc
    19741 gaaacggagc cgatgcatgg tttacaggtg atgacaggtt tcaccctgcg ccctgaccgg
    19801 gcggcactgg aaatcgccag ccgcgtctat aacggcaacg ccacgccgcg tcatttcttg
    19861 tggtgggcca acccggcagt gaaagggggt gaagggcatc agagcgtttt cccaccggat
    19921 gtgactgcgg tgtttgatca cggcaaacgg gccgtctccg ctttccccat cgccaccggc
    19981 acttactaca aagtggacta ctccgccgga gtggacattt ctcgctataa aaatgtgcct
    20041 gttccaacct catatatggc cgaaaaatcg cagtacgatt ttgtcggcgc gtggtgtcac
    20101 gatgaagatg gcggtttgct gcacgttgcc aaccaccata ttgcgccagg taaaaaacag
    20161 tggagttggg gacacagtga atttggccag gcgtgggata agagtctgac cgacaataac
    20221 ggcccgtata tcgaactgat gaccggtatt tttgccgata accagcctga ttttacctgg
    20281 cttgatgctt acgaagagaa gcgtttcgag cagtatttcc tgccttatca ttctctgtgc
    20341 atggtgcaaa atgcctcccg cgatgcggtg ataaaactcc agcgtagtga gcgggggatt
    20401 gagtgggggc tgtatgccat ctctccgttg aacggatacc gtctggcgat ccgcgaaatc
    20461 ggcaaatgca acgcgttgct cgatgatgcc gtggccctga caccagcgac cgccatccag
    20521 ggcgtgttgc acggtatcaa tcctgacaga ctgaccattg aactctccga tgccgacggc
    20581 aatattgtac tgagttatca ggaacatcag ccgcaggagt tgccgttgcc ggacgtcgcc
    20641 aaagcgccac tggcagcaca agacattacc agtacagatg aagcctggtt tatcggtcag
    20701 catctggagc aatatcatca cgccagccgt tcaccgttcg attactacct gcgcggcgtg
    20761 gcgctggacc cgctggatta tcgctgtaac ctggcgctgg cgatgctgga atataaccgc
    20821 gcagatttcc cgcaagcggt ggcgtatgcc agtcaggcgc tgaaacgcgc acatgcgctg
    20881 aacaaaaacc cgcagtgtgg acaagcaagt ttgattcgcg ccagtgctta cgaacgtcag
    20941 ggacaatatc aacaagccga agaggatttc tggcgtgcgg tctggagcgg caatagtaaa
    21001 gcaggcggct attatggtct ggcacgactg gcggcgcgta atgataactt cgacgcgggt
    21061 ctggattttt gccaacaaag tcttcgtgcc tgcccaacca atcaggaagt gctttgcttg
    21121 cataatctgc tgctggtgtt aagtggtcgt caggacaacg cgcgtttgca gcgcgagaaa
    21181 ctgctgcgtg attatccgct gaacgccact ctgtggtggc tgaactggtt cgatggtcgt
    21241 agcgaatcag ccctcgcgca gtggcgcggt ctgtgtcagg gacgcgacgt taacgctctg
    21301 atgaccgccg ggcaactgat taactgggga atgcccaccc tggcggcaga gatgctgaac
    21361 gcactggact gccagcgcac gctgccgctt tacctgcaag ccagcttgct gccgaaagcc
    21421 gaacgtggcg aactggtcgc aaaagccatt gatgtcttcc cgcagtttgt ccgtttcccg
    21481 aatacgctgg aagaagtggc ggcgctggag agtattgaag agtgctggtt tgctcgccat
    21541 ttactggcct gcttctacta caacaaacgt agctacaaca aagccattgc cttatggcaa
    21601 cgttgcgtag agatgtcgcc ggagtttgcc gacggctggc gcgggttagc gatccatgcg
    21661 tggaataaga aacacgatta tgagctggcc gcgcgttatc ttgataatgc ttatcagctt
    21721 gcgccgcagg atgcacgtct gcttttcgaa cgggatttgc tggataagtt aagtggagcc
    21781 acaccggaga aacgactggc gcgtctggaa aataatctgg aaattgcgct gaaacgcgac
    21841 gacatgaccg cagaactgct caatttgtgg catctcacgg gtcaggcaga caaagcggcg
    21901 gacattctcg ccacgcgtaa attccacccg tgggaaggcg gggaagggaa ggtcaccagt
    21961 cagtttatcc tcaaccagtt attacgcgcc tggcagcatc ttgatgccag agagccgcag
    22021 caggccagcg aactgcttca tgccgcgctg cattatccgg agaatttaag cgaaggccgt
    22081 ttaccggggc aaactgataa cgacatctgg ttctggcagg cgatatgcgc caacgcgcag
    22141 ggcgatgaaa ctgaagcgat gcgttgttta cgtctggcgg cgaccggcga tcgcaccatt
    22201 aacatccaca gttattacaa cgatcagccg gttgattatc tcttctggca aggaatggcg
    22261 ctgcgactgc tgggtgaaca gcaaaccgca cagcaactgt ttagtgaaat gaaacagtgg
    22321 gcgcaagaga tggcgaaaac cagtattgag gcggatttct ttgctgtttc acaacctgac
    22381 ctgttatcgc tgtatggcga tttacaacag cagcataaag aaaaatgcct gatggtggcg
    22441 atgctggcgt ccgcgggact cggggaggtt gcgcaatatg agtctgctcg cgctgaattg
    22501 acggcgatta atccggcctg gccgaaagcg gcattattca ccactgtgat gccttttatt
    22561 tttaactacg
//
LOCUS       DAJTYG010000060        22319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTYG010000060 DAJTYG010000000
VERSION     DAJTYG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.060"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(21..692)
                     /gene="queE"
                     /locus_tag="OO568_002495"
     CDS             complement(21..692)
                     /gene="queE"
                     /locus_tag="OO568_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR6641812.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            831..971
                     /locus_tag="OO568_002496"
     CDS             831..971
                     /locus_tag="OO568_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641813.1"
                     /translation="MSEENKENGFNHVKIFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            985..1857
                     /locus_tag="OO568_002497"
     CDS             985..1857
                     /locus_tag="OO568_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR6641814.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1917..3215)
                     /gene="eno"
                     /locus_tag="OO568_002498"
     CDS             complement(1917..3215)
                     /gene="eno"
                     /locus_tag="OO568_002498"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6641815.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="OO568_002499"
     CDS             complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="OO568_002499"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6641816.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVVGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="OO568_002500"
     CDS             complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="OO568_002500"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6641817.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6055..8289)
                     /gene="relA"
                     /locus_tag="OO568_002501"
     CDS             complement(6055..8289)
                     /gene="relA"
                     /locus_tag="OO568_002501"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HCR6641818.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LYGS"
     gene            complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="OO568_002502"
     CDS             complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="OO568_002502"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6641819.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9695..12451
                     /gene="barA"
                     /locus_tag="OO568_002503"
     CDS             9695..12451
                     /gene="barA"
                     /locus_tag="OO568_002503"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6641820.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="OO568_002504"
     CDS             complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="OO568_002504"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6641821.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14044..15384)
                     /locus_tag="OO568_002505"
     CDS             complement(14044..15384)
                     /locus_tag="OO568_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6641822.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15386..16738)
                     /gene="gudP"
                     /locus_tag="OO568_002506"
     CDS             complement(15386..16738)
                     /gene="gudP"
                     /locus_tag="OO568_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6641823.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLRSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGQFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17172..17621)
                     /locus_tag="OO568_002507"
     CDS             complement(17172..17621)
                     /locus_tag="OO568_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6641824.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17639..18421)
                     /gene="truC"
                     /locus_tag="OO568_002508"
     CDS             complement(17639..18421)
                     /gene="truC"
                     /locus_tag="OO568_002508"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6641825.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGERLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18421..18750)
                     /locus_tag="OO568_002509"
     CDS             complement(18421..18750)
                     /locus_tag="OO568_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6641826.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19372..19917)
                     /gene="syd"
                     /locus_tag="OO568_002510"
     CDS             complement(19372..19917)
                     /gene="syd"
                     /locus_tag="OO568_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6641827.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19985..20833
                     /gene="queF"
                     /locus_tag="OO568_002511"
     CDS             19985..20833
                     /gene="queF"
                     /locus_tag="OO568_002511"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6641828.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20945..22309
                     /gene="ppnN"
                     /locus_tag="OO568_002512"
                     /gene_synonym="ygdH"
     CDS             20945..22309
                     /gene="ppnN"
                     /locus_tag="OO568_002512"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6641829.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5510 a         5916 c         5294 g         5599 t
ORIGIN      
        1 taacgcttat aaatgtgtaa tcaggcaata tttaaatatt tatgtgtttg catcgacaaa
       61 cgccaattac gcgcaataca ggtttcaatg cacaatcgtg tggcatcatc cttttggcta
      121 atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc
      181 agtgcttcaa tatcgcgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt
      241 gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac ggtaacccag
      301 gtattcggtg tgcagcgtac ttcatgagtt ccactggttt cgatctggca gctaaaaccg
      361 ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc accacccgta
      421 atcaccacat gccgcgcggt atatccctga cgaccaatga cagccagcaa gtcttcactg
      481 ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa aagggagact
      541 tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg
      601 catccctgta aacgaataaa aatggcgggg acgccggtaa agtaaccctc accttgcagg
      661 gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg gataattgtt
      721 aattattgca gatcctgccg caacaatcat gtcttattaa cattctgtta catgcagatt
      781 taaagaaaaa tacgaaacat atcgttaata attaaaggga gtaacgtatt atgtcagaag
      841 aaaataaaga aaatggattt aatcatgtca aaatattcac caaaattata tttatttttt
      901 ctgtattagt ttttaatgat aacgaatcta aaattaccga tgccgccgtc aatttattta
      961 tccagattta atgagaaatt tataatgcgc tatttcatac taatgttcac tttcgtatgt
     1021 tcctttgttg cagcccaacc aactattgtc ccacaattac agcaacaagt tacgggtctt
     1081 acgagtagct taaactcaca agaaaagaaa gaactgacgc acaagttaga atctattttc
     1141 aataataccc aagtgcaaat tgctgtatta attgtttcca caaccaaaga tgagacaatt
     1201 gaacaatatg ctacaagagt ttttgacaat tggcgtttag gagatgccaa acgtaatgat
     1261 gggatactga tcgttgttgc ctggtcggat cgcactgtcc gcatccaggt aggttatggg
     1321 ctggaagaaa aggtaaccga tgctctggct ggagatatca tccgtagcaa catgataccc
     1381 gcatttaaac aacaaaaatt agctaaggga ttagagttag ctataatcgc tttgaataac
     1441 caactcactt cacaacacca atatccggct aatccttcag aaagtgaatc aacgtcttcc
     1501 actgatcatt attactttgc tattttttgg gtatttgcag tgatgttctt ccctttctgg
     1561 ttttttcatc aaggcagtaa tttttgtcgc gcatgtaaaa gtggagtctg tatttcagca
     1621 atccatcttt tagatttatt cctgttctcc gataaaactt tttccattgc tgtattttcc
     1681 ttctttttta ctttcaccat atttatggtc tttacctgtt tatgtgtact tcagaaaaga
     1741 gcatctggta gaagttatca ttcagacaac agcggttccg ctggaggctc aagtggcggc
     1801 ggatttagcg gcgggggagg ttcttctggc ggtggcggcg catccggccg ctggtaactc
     1861 caccataaaa aatgccagcc cgaaggctgg cattatcaaa tcagataaag tcagacttat
     1921 gcctggcctt tgatctcttt acgaccgttg tacggtgctt tttcgcccag agcttcttcg
     1981 atacgaatca gctggttgta tttagcaaca cggtcagaac ggctcataga accagttttg
     2041 atctggcctg cagcagtacc aacagccaga tcagcgatgg tagcatcttc agtttcacca
     2101 gaacggtgag agataactgc agtgtagcca gcatctttcg ccatcttgat tgcagccaga
     2161 gtttcggtca gagaaccgat ctggttgaat ttgatcagga tggagttagc gatacctttt
     2221 tcgatacctt ctttcaggat cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg
     2281 attttgtcgc ccagaacttt ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga
     2341 ccgtcttcga tagaaacgat cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat
     2401 tcttcagagg tgaacgcttt gttgccttcg ccagccagaa cgtatttacc atctttgtag
     2461 aattcagaag ctgcgcagtc catcgccaga gtgatgtctt tgcccagttc ataaccagca
     2521 gctttaacag cttcagcgat aacagccaga gcttcagcgt tggaacccag gttcggcgca
     2581 tagccacctt cgtcaccaac agcagtgttc atgcctttcg ctttcagaac ttttgccaag
     2641 tgatggaaaa cttcagaacc catgcggatg gcttctttca cagttttcgc gccaaccggc
     2701 tgaatcatga attcctggat atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg
     2761 ttcatcatcg gaaccggcat ggagtatttg cccggagtgc cgttcagttc agcgatgtgc
     2821 tcgtacagcg gcataccttt agcagctgca gcagctttgg cgttagccag agatacagcc
     2881 aggattgcgt tcgcgccgaa tttggatttg ttttcggtgc cgtccaggtc gatcatgatc
     2941 ttgtcaatgc cagcctgatc tttagcatct ttgccaatca gcgcctgagc gatcgggccg
     3001 tttaccgcag caacagcttt ggttacgcct ttacccagga aacgggattt gtcgccatcg
     3061 cgcagttcca gagcttcacg ggaaccagta gaagcacctg acggagcagc tgccataccg
     3121 acgaaaccac cctccagatg tacttcggct tcaacagtcg ggttaccacg ggagtcgatg
     3181 atttcacgac cgatgatttt tacgattttg gacattaggt tttcctcaag tcactagtta
     3241 aactgaaact ccagacaaac aacgcgtacc caaggtacgc gttgccgttc taactttttt
     3301 acttacttcg cctgacgttt ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc
     3361 gggtgaccat cacgtggagt agaagtaaac tccggatgga actggcaagc cacgaaccac
     3421 gggtgattcg gaacttcgat gatctcgacc aactgatcat ccccggaacg gcccacaacg
     3481 cgcagacctg catcttcaat ctgtttcaac agcatgttgt tgacttcgta acggtgacga
     3541 tgacgctcaa caattgtcgg cgcattgtat agctggcgaa ccaggctatc gtcaaccaac
     3601 tggcactgct gtgcgccgag acgcatggta ccgccgagat cgctcttctc gctacgaact
     3661 tcaacgttgc cgttttcatc gcgccactcg gtaatcagcg ccacaaccgg gtacttacag
     3721 tctggcacaa attctgtaga gttggcgttc tccatgttgg caacatggcg agcgtaatca
     3781 attaacgcca cctgcatacc caggcaaatg cccagataag gaatattgtt ctcacgcgca
     3841 aaacgcgcgg tcgtaatcat gccttctacg ccacgatagc cgaaaccgcc aggtacgagg
     3901 attgcgtcca gacctttaag gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc
     3961 agtttgatgt tgacgctgac acgattcttc agcccaccgt gtttcagtgc ttcgatcact
     4021 gatttataag catccggcag ttcaatgtac ttgccgacca taccgatggt gacttcactt
     4081 accggattcg cttcttcgaa gataacctgt tcccattcgg acagatttgc ttccgggcag
     4141 tttaagctga atcgtttaca aatataatcg tccagcccct gagatttcaa caggcccgga
     4201 attttataga tggaatcgac gtctttcaga gaaataaccg ctttttccgg aacattacag
     4261 aacaatgcaa tcttcgcacg ttcgttcgcc ggtacagcgc gatctgaacg acaaatcagg
     4321 atgtcaggct ggataccgat ggagagcagc tcttttacag agtgctgagt cggtttggtt
     4381 ttgacttcac cagacgctgc catgtacggc accagcgtca ggtgcataaa cagagtgtgc
     4441 tcacggccaa tttcaacagc catctggcga atagcttcga ggaacggcaa ggattcgata
     4501 tcacctactg taccgccgat ttctaccagt actacgtcat gaccttcgcc accttccagc
     4561 acgcgctctt tgattgcgtt agtgatgtgc ggaataacct gcacggttgc gccgaggtag
     4621 tcaccgcggc gttctttacg cagaacgtca gagtagatac gacccgtggt gaagttgttg
     4681 cggcggctca ttttggtacg aatgaaacgc tcgtagtgcc ccaggtccaa gtcggtttca
     4741 gcgccgtctt cagtaacgaa cacttccccg tgttggattg ggctcatagt acctggatcg
     4801 acgttgatgt aaggatccag tttcatgatg gtcacattga ggccacgggc ttcaagaatg
     4861 gctgcgaggg aggctgcggc aatgccttta cccagagagg atacgacccc gccggtcaca
     4921 aaaatatagt tcgttgtcat gctgaacctg agaagttagg ttgaaaagac gatggaataa
     4981 ccaagacggg aaagcagtat acccgaacat gacctgtgcc acaaactttc attatccctc
     5041 ctcttcgcca gcgcactatt gaaatcagga gtgagaaaat agcccctttg gggtaaatgt
     5101 ttttgacgca aatcaagcgc ttgtcattta aaaaatgaca caaatggcgc ttgaccgcgt
     5161 aattccctta gagatcaatt tcctgccgtt ttacctgttg ccagacttct tccattgttt
     5221 cgaggtcaac acctgtcatt tccagtccac gcgcggcaac aatacgctcc acttcgcgaa
     5281 aacgacgctc gaatttttcg ttcacttttt gcaatgcgat ttctgctttc gtccctaaat
     5341 ggcgagccag attaaccgtg gcaaacagca ggtcccccat ttcctcctcc agtttagcct
     5401 ggtcgacaac agcctgccgc gcctcgtaca tcacctcgtc gatctcttcg tagactttat
     5461 caaccaccgg gccaagcgtc gtccagtcga agccaacgtt ggcgcaacgt ttctggattt
     5521 tttgcgcccg cattaaagcc ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg
     5581 ctttctgcgc gcgctcttcg gttttgattt gctcccaacg ggcaagcact tcactactgt
     5641 tttcggcaga actatcagca aaaacatgcg gatggcgacg ctctaattta tcgctaatag
     5701 cagcgcaaat atcattaaag tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca
     5761 ccacctggaa tagcagatcg cccagttcac cgcgaagatc gtcaaaatct tcacgggcga
     5821 tggcgtccag cacttcgtag gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct
     5881 gctctttatc ccacgggcag ccgttttccg gatcgcgcag gcgctgcata atagtgagca
     5941 aacggtcgat ttgattcatt gaattgtcct gaaaattgcg ggtctgtcag gtgtaatacc
     6001 ttcgagtaaa agatatcgct ggatacggct gacgccatat ccagctatgt aagactaact
     6061 cccgtacaac cgacgcgcgt cgataacatc cggcacctgg ttgagtttac ccagcacgcg
     6121 ccccagcact tgcaggttgt aaatctcaat ggtcatgtcg atggtcgcca gttgctgttt
     6181 ggtgtcgcta cggctggcaa cgccaagcac gttcaccttc tcgttggcga gaatggtcgt
     6241 gatatcacgt aacaacccac tacgatcatt agccaccacg cggaccacca gcgaatatcc
     6301 ggcggagtag ctctcacccc ataccgcgtc aacaatgcgt tctggcgcat gggagcgcag
     6361 ttccgccagt tgttcgcaat cggcgcggtg tactgaaata ccgcgtccct gggtaatgaa
     6421 gccgacaatc tcatctccag gaatcggctg gcagcagcgc gcgatgtggt gcatcaggtt
     6481 accaacacct tcaaccacca cacgaccgtt atctttactg cggttttgcg gtgtgtagct
     6541 tttttgctga agctgtttca gcgcggcggc gtcctgctct tcggcactcg gcttattaaa
     6601 ttgcgattgc aggaagttca ccatctgatt gagacggata tccccgccac caatcgccgc
     6661 cagcaactcg tcgacatcat tgaagttgta acgcggcagc agatgttttt ctgcttcttt
     6721 caggctgatc cccagatgtt ccagctcgtc gtcaaggatt tgccgcccag ccagaatgtt
     6781 tttgtcacgg tcctgtttac ggaaccaggc gtgaattttc gaacgcccac ggctggttgt
     6841 gacgtaaccg aggtttgggt ttaaccagtc acggctgggg ttcggctgtt tctgggtgat
     6901 aatttcaatc tggtcgccca tttgcagctg gtaggtgaac ggcacaatgc gcccgccaat
     6961 ttttgccccg atgcagcggt gtccgacatc actgtggatg tggtaagcga agtccagcgg
     7021 cgttgatccc gcaggcaaat caacgacatc acctttcggc gtaaagacat acacccggtc
     7081 gtcaaacacc tgactacgta cttcgtcgag catttcgccg gaatcagcca tctcttcctg
     7141 ccacgcaatc agtttacgca gccaggcaat ccggtcttca tgtcccgaac gtgcgccgcc
     7201 agctgccgcg ccctctttat atttccagtg cgcagcaaca cccaactccg catcttcatg
     7261 catctgtttg gtgcggattt ggatctcaac ggtttttcca cccggcccca gaaccacggt
     7321 atgaatagac tgataaccgt ttggtttcgg gttagcgacg taatcgtcaa actcatccgg
     7381 caggtggcga tagtgagtgt gcactatccc cagtgcggca taacaatcct gtaaacgctc
     7441 ggcgacaata cgtaccgcac gcacatcaaa cagctcatcg aaggcgaggt tctttttctg
     7501 cattttgcgc cagatgctgt agatgtgttt cggacgacca tacacttcag ctttaacgcc
     7561 ttcagctttc atctcagcgc gcagatggcc gacaaactct tcgatatagt gttcgcggtc
     7621 gagacggcgt tcatgcagca gttttgcgat gcgtttgtat tcggtcgggt ggagataacg
     7681 gaagcagtaa tcttccagct cccatttcag ttgcccaatc ccaagacggt tcgccaacgg
     7741 cgcgtagata ttggtgcact cttttgccgc cagtacacgt tcatcttccg gcgcatcttt
     7801 tacttcgcgc agatgagcaa tacgctccgc cagtttgatg actacgcagc gaaaatcatc
     7861 gaccatcgcc aataacatcc ggcgaacgtt atcgacctgt tcggaggaaa cagaatcagt
     7921 gtgcgtcgct ttcagctggc ggatcgccgc catatcacgc acgccgtgaa taaggttaac
     7981 aaccgactta ccgacgctct cacgcagcac atcttcgctg actacgttgg catcagccag
     8041 agggaacagc agcgccgccc gcagcgtgtc aatgtccata cttaatgtcg agaggatctc
     8101 caccatctca acaccacgcc acaataacag actggcatcc ggatgcccct gcgtctgttg
     8161 cagacaatac gcccaggttt cggctaagca ctcacacgac ttctggctgg taatacccag
     8221 acttgcgatc catttttccg gatcaaattc accagcctta ttgatatgtg cacttcttac
     8281 cgcaaccatc gtcctctcct ttagggacca gacctgccga aatcggcaaa tcgtaactat
     8341 ttaacgcgcg agaaaagtac catcgattcc agatgtcccg tgtgtgggaa catatccagc
     8401 atcgccagtc gcgcaatggt atatcctgct tttaataacg cttcgctatc ccgagccagc
     8461 gttgcagggt tacaggatac ataaactata cgaataggtt ccagttttat aatttgctgc
     8521 ataacacctg cggcacctgc tcgcgccggg tccagcaaca ctttatcgaa gccgtttttc
     8581 gcccacggct gctttgtgac atcttcttca agattttcgt gataaaacgt cacattctgt
     8641 aagccattaa gacgcgcatt ctgctggcct ttttccacca gcgccggaac accttctaca
     8701 ccgaccacac tggcagcttg tgtcgccaat ggcagtgtaa agttgcccat accgcagaac
     8761 agatccagta cgcgatcttc aggttgcaca tccagccatt ccaacgcacg cgctaccatt
     8821 ttttggttca cacccgcatt gacctgaata aaatcgcgcg ggctaaaagt taagcgcaac
     8881 ccgtttgagt cataccaggg catctcacca gagacggttt cgagtatctc actatcgggg
     8941 gcgagataca gatccaggcc ttcagaatgc gaaaagcgtt ccagtttttc gcgatctgcc
     9001 gaacttagcg gtgcggtatg gcgcaaaatc atcagcgtgc cgctggttgc ctgtaccagt
     9061 tcaacatgac caagatggcg catagcttgt aagctgccca gacatgccct gactttgggc
     9121 agcaatgctt caagttgggg cgctaaaatg gggcattgtt tgacgtcgac aatgtcactg
     9181 gagcccgctt tgcgaaaccc catctgaagt tgctgtgttt tcggtaagta gttcagactt
     9241 aaacgcgcgc ggcggcgata gccccaggga acatcggcga tcacttcaga gacatcgtgt
     9301 ttcattaatc gggcgagtgc cgcacttttg cttcgctgct gtaaatccac gctggcgtgt
     9361 tgttgctgac agccgccgca tacgccaaaa tgaggacagc gtggcgtttc gcgttccggg
     9421 ctatcgctta accggcgtac gactttagcg cgggcatact gttttttatc ttcagtaaca
     9481 gcaacttccg cgttttcctg cggcaataat ccggggataa ataacgtttt gccgttatgt
     9541 cgcgccacgc cctgaccaaa agagtcgagg tcgttgactg aaacggttat gatctgacgc
     9601 gtcgtcgtgc gtcgttttgc agagtagaat tgcgccattg gcgagacttt ctcaatttaa
     9661 cagtgtgacc ttaattgtcc cataacggaa ctccatgacc aactacagcc tgcgcgcacg
     9721 catgatgatt ctgatcctgg caccgaccgt ccttattggt ttattgctga gtatcttttt
     9781 cgtcgtgcat cgctataacg acttgcagcg tcaactggaa gatgccggtg ctagcattat
     9841 tgagccgctt gcagtttcta ctgaatatgg catgagcctg caaaatcgcg aatctatcgg
     9901 tcagttaata agcgtactgc atcgtcgcca ttccgatatt gttcgcgcga tttcggttta
     9961 tgatgaaaat aaccgactct ttgtcacctc caattttcat cttgatccct catcaatgca
    10021 gctcggcagc aacgtgccgt ttcctcgcca gctcactgtc actcgtgacg gcgatattat
    10081 gatcctccgc acgccgatta tttctgagag ttactccccc gacgaatcgc ccagtagcga
    10141 tgccaaaaat agtcaaaata tgttgggata tattgcgctt gagctggatc ttaaatcggt
    10201 tcgcttgcag caatataaag agatctttat ttcctgcgtg atgatgctgt tttgtatcgg
    10261 tattgcgctt atttttggct ggcgcttaat gcgcgatgta accggtccga ttcgcaacat
    10321 ggtgaatacc gtcgaccgca tccgtcgcgg gcaactcgac agccgggtgg aaggatttat
    10381 gctcggcgag ctggatatgc tgaaaaacgg tatcaactcg atggcaatgt cgctggctgc
    10441 ttatcacgaa gagatgcagc acaatatcga ccaggcgacg tccgatctgc gtgaaacgct
    10501 ggagcagatg gaaattcaga acgttgagtt ggatctggcg aaaaagcgcg cccaggaagc
    10561 ggcgcgtatt aaatccgagt ttctggcaaa tatgtcacac gagctgcgta caccactgaa
    10621 tggtgttatt ggctttaccc gcctgacgct gaaaacagaa ttaacaccaa cgcagcgcga
    10681 tcacctgaat acgattgaac gttcggcaaa taatttgctg gcaattatta atgatgttct
    10741 cgacttctcg aaactggaag caggtaagct gattctggaa agtattccat tcccactacg
    10801 cagcacgctg gatgaagtcg ttactctgct ggcacattct tctcacgata aagggttgga
    10861 actgacgctc aatattaaaa gcgacgtgcc tgataacgtg atcggcgacc cactgcgatt
    10921 acagcaaatc atcactaacc tagtggggaa tgcaattaaa ttcaccgaga atggcaacat
    10981 tgatattctg gtagaaaaac gtgcgctgag taataccaaa gtgcagattg aagtgcagat
    11041 tcgggatacc ggcattggta ttcctgaacg cgatcaatcg cgcttattcc aggccttccg
    11101 acaggctgat gccagtattt cccgccgtca tggtggcacc gggctggggc tggtgattac
    11161 acaaaaactg gttaatgaaa tgggcggcga tatttcgttc catagccagc cgaatcgcgg
    11221 ttcaactttc tggttccaca ttaatctcga tctgaacccg aacattatta tcgaagggcc
    11281 atccatccag tgcctcgcag gtaagcgcct ggcctatgtc gaaccaaact ccgcagcagc
    11341 gcaatgcacg ctggatattt taagtgaaac gccgctggaa gtggtttata gcccaacgtt
    11401 ctccgcgctg cctcccgcgc attacgacat gatgttgtta ggcatcgcgg tgaccttccg
    11461 cgagccgcta acaatgcaac atgagcgatt agcgaaagcg gtatcgatga ccgatttcct
    11521 gatgctggca cttccttgcc atgcacaagt caatgctgaa aaactcaagc aagatggtat
    11581 cggcgcgtgt ctgctgaaac cattaacacc tacgcgcctg ttgcctgccc tgacggaatt
    11641 ttgtcatcac aaacaaaaca cgcttttgcc tgtaaccgat gaaagtaagc tggcaatgac
    11701 agtcatggcg gttgatgaca accccgctaa cctgaaactt atcggcgcat tgctggaaga
    11761 tatggtgcaa catgtggaac tttgcgatag cgggcatcag gcagttgaac gggcgaaaca
    11821 gatgccgttc gatttgatct taatggatat tcaaatgccg gacatggatg gcattcgggc
    11881 ctgcgaactc atccaccaac tcccgcatca gcgacaaacg ccggttatcg cggtaacggc
    11941 gcatgcaatg gccgggcaaa aagagaagct gcttggcgca gggatgagcg attatctggc
    12001 gaaaccgatt gaagaagagc gattgcataa tttgttgttg cgctacaagc ctggcagcgg
    12061 tatttcgtct cgcgtcgtga cgcccgaagt caacgaaatt gtggtgaacc cgaatgcgac
    12121 cctcgactgg caactggcac tacgccaggc agcaggaaaa accgatttag cgcgcgatat
    12181 gctgcaaatg ttactcgatt tcctgcctga agttcgcaac aaagttgagg aacagctggt
    12241 tggagaaaac ccggaaggcc tggttgattt gattcataaa ctgcatggca gttgcggcta
    12301 tagcggcgta ccacgtatga agaatctctg ccagctgatc gaacaacagc tacgtagcgg
    12361 tactaaagaa gaagatttgg agccggagct gctggaactg ttggacgaga tggataatgt
    12421 cgcgcgcgaa gccagcaaaa ttctcgggta acggacgtct tgtcgagtag caatcggctt
    12481 tttatgacgc tggtttcaga ttggtgacaa agtgcctgtg tttatgccgg atgcggcgtt
    12541 aacgccttat ccggcctaca aaaccaatta aattcaatga attaaaaaat gatgtagacc
    12601 tgatacatag cacaacaggc tattttgcgt ttagcatcag tctcaaaccg gctccagata
    12661 gagccggttt tggttttctg tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc
    12721 agccaggaat cagatactgc attcccatcg catcgtcacg cgcgccaagc ccgtgtttct
    12781 gatacagctc atgggctttc atcacttgat ccatatcgat ttctacaccc agccccggtt
    12841 tttctggcac ctgtaccagc ccgcctttga tctcaaacgg ttctttggtc aggcgctgat
    12901 taccttcctg ccagatccag tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg
    12961 caacatgggt aaacatcgcc agggaaatat cgaagtggtt gtttgagtgt gaaccccagg
    13021 tcaggccaaa ttcatggcac atttgcgcca cacgtaccga accttgcata gtccagaaat
    13081 gcggatccgc cagcgggata tcaacggatt gtaacgagag cgtatggccc atctgccgcc
    13141 agtcggtggc gatcatattg gttgcagtcg gcaggccagt cgcgcgacgg aactctgcca
    13201 tcacttcacg cccggagaaa ccttgctccg caccacacgg atcttctgca taagccagcg
    13261 aacctttcag gtatttacca attttaattg cttcgttcag cgaccaggca ccgttaggat
    13321 cgagcgtaat acgcgcctgc gggaagcgtt gcgccagtgc cacaatagac tcggcttctt
    13381 cttccccggc cagtacaccg cctttcagtt tgaaatcgtt gaagccatat ttttcatacg
    13441 ccgcttccgc caggcgcacc accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat
    13501 accagtcgca tgagtcatcc ggctggcttt gatacggcag aggtgtggct ttgcgattac
    13561 cgacgaagaa cagataaccg agcatttcga cttcgctacg ctgttgacca tcgcccagca
    13621 gcgatgccac gtttaccccc agatgctgcc ccagcagatc cagcattgcc gcttctatcc
    13681 cggtaactac atgaatagtg gtacgcaaat caaatgtctg caaaccgcgc ccactagcat
    13741 cacgatcggc aaaagtatta cgcaccagcg tcagaacgtt tttgtattca cccagcgttt
    13801 tacctaccac cagcggaatc gcatcttcca gcgttttacg gattttctcg ccgccgggaa
    13861 tttcccctac gccagtgtga ccggaattat ctttgataat cacaatatta cgcgtgaaga
    13921 acggtgcgtg tgcaccactc agattcatca gcatactgtc atgacccgcc accgggatga
    13981 cctgcatttc agtaacaaca ggcgtcgtaa attgagaact catatttatg tcctttttca
    14041 gaatcaatga cggccgaaaa cgggacgttt acggtcaaag gtccagccgg ggatcaggta
    14101 ctgcatcgga cctgcgtcgt tacgcgcacc gcaaggcaga cgtttatagg cctcatgtgc
    14161 cttttgtacc tgttcccagt ccagttccac gcccagaccg ggcgcatcag gaacggcaat
    14221 ttttccgttt ttaatctcca gcggattttt ggtcaggcga caatcgccct cctgccaaat
    14281 ccagtgggta tcgatagcgg taggattacc cggtgccgcc gcgcccacat gggtaaatat
    14341 cgccagagag atatcgaaat ggttgttgga atggcagccc caggtcagcc cccagtcgtc
    14401 gcaaagctgc gccacgcgga ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg
    14461 aatatctacc gcattgagca tcaccgcatg acccatttcg cgccagttgg tggcgatcat
    14521 gttagtcgcg acgggcaagc cggtcgcccg tcgaaactcc gccatcactt cacgtccgga
    14581 gaagccctgt tctgcgccgc atggatcttc ggcataggta agaacatcat tcagcccttt
    14641 gcataaagaa atggcttcat caagcagcca tgcaccgttg ggatcaacgg taatccgcgc
    14701 atccgggaag cttttcttca atgcacgaac agtgtcgatt tcttgctcgc caggtaacac
    14761 cccgccctta agtttgaaat ctttaaagcc atagcgatcc tgtgaggctt ccgccagacg
    14821 cacaacggct tcgctgttca tcgctttctg atggcgcaac tgataccact catggttgcc
    14881 cggcgtattt tccagataag gaagatcggt tttggtccga tcacctatat aaaacagata
    14941 accgaggacg gtaatagtct cgcgttgctt gcctggcccc aacagttcgc agaccggaac
    15001 attcagcgcc ttacctagca ggtcaagcaa ggcggcttcc agcgccgcca cggcattaac
    15061 gcgcaattca aaagtccagg cacctttgcc gaaggtctca aaatcggctg cctgattacc
    15121 tttatgtacc tgctgaacca ctttattcag gcgcgcaact tcctggccca gaaccatcgg
    15181 aatagcatcg accagtgttt gataaatcac ctctccgccc ggcgcttcac caatgccggt
    15241 atgcccggcg ttatcggtga gtaccacgat attgcgggtg aagtatgcgt tatgcgcacc
    15301 accaatattg agcaacatgc tgtcatgtcc ggcgaccgga atgactttca tatcagtaat
    15361 aacaggactg gattgtgtcg ccatcttatt gccccgcgac aggtttcagc tcgatacgct
    15421 tgatatcgcc caccagcacc aggtagctca gtaccgcgat tagggcatga acaccaacat
    15481 aaatcagcgc cccattaaac gagccagtcg tgccaacgat ataaccaatt gcgattggcg
    15541 tgacgatgcc agaaatgtta ccgaacatgt tgaactggcc accggaaaga ccgctgatct
    15601 cttttggcgc ggtatctgcc attactgccc aacccagcgc cccgatgccc ttaccgaaga
    15661 aggccagcgc cataaagccg atgatcatcc actcaacgtt gacgtagttg cagaacacca
    15721 tcaccatcga cagcaacatg cccattacga tcggtgtttt acgcgcaatg ttcagcgatc
    15781 ccgtgcggcg catcagccag tcggaaataa ttccacccag cacaccgccg ataaaaccgc
    15841 aaaccgccgg aacggaagcc acaaagcccg ctttcagaat cgacatccca cgcgcctgca
    15901 ccagataaac cggaaaccag gtaatgaaaa agtaagtcag ggcgttgata cagtactgac
    15961 cgatataaac gccgatcatc atccgtgacc tgagcagctg tttgatctgc ccccacttca
    16021 cgctgaacgg cactttaact ttggtgcttt gctgatccat attgatcagc gcgccacccg
    16081 cggcgatata ctccagctct ttctgattta cccccggatg ttgatttggc tcgtggatga
    16141 ctttcaacca gataaagctg atgacaatgc ccagaccgcc cataaagaag aagacgtgtg
    16201 accagcccac ttcatgcgtc agccagccca taatcggcgc gaagatcacc gttgcgaagt
    16261 attgagcgga gttaaaaatc gacaccgccg ttcccctttc ctgcgccgga aaccaggccg
    16321 caacaatgcg actgttgccg gggaaagatg gcgcttcagc aagcccgacc aggaagcgca
    16381 gagtaaacag ggcaacgata atgccgaatc cactaaagat atcgacgaag ccttgcagca
    16441 aggtaaacat cgaccagata aagatcgacc agaagtagac gcgttttgaa ccaaaacgat
    16501 ccagcaacca accaccaggg atctgcccga taacataagc ccatgagaag gcagagaaca
    16561 catagcccat tcccacggga tcaaggccga tatctttggc catttccgaa ccggcaatag
    16621 aaagcgtagc gcggtcgccg tagttgaagg atgtgacgat aaacaacatc accactatcc
    16681 agtaacgagc attcgtgcgt ttttccacac tgctcgcagc ctgacttaaa gaactcattg
    16741 ttgcactcct gaaaattcgc gttagccacg ctcactctgg actgcgacat cgccaggaaa
    16801 tcagaggtga cgtagggtgt tttttgccgt ttttataggt cgttcgccga atacggcgcg
    16861 tgtttatatc tggcaatagc agtataaaaa gcgcgccata gcggctcacc gtgcaacaac
    16921 acaacattaa tgcgttcaat gaggcccgat tttggcatta gccctggacg gtggaatcca
    16981 cttcacggaa atgaaaacaa gaacaagaaa ggaagggtaa aaacgaagaa ataaagaaga
    17041 gtatgaaatg gatcgcttga ctctaggcaa acgccagtaa aaatccgcgc tatgaagcag
    17101 tttttactgg catttgcctg aaaagattcg attcagcacc gctaaaacga catttaccgc
    17161 tcgctgacat atcaggacaa cagcgtgccc cactgttcga cccacggatt tgattccgtt
    17221 tccggttccg ggttctcact ggcgtcaatc aacagcattt cgccaacccg ctgagcgctc
    17281 tgttcctgca acaaggcatc gaactgtttg ccgccattgc aaaaatttac ataactgctg
    17341 tcgccgagcg caatcacgcc ataacgcaga ttcggctgga agcccagact atctttgatt
    17401 ccctgaaaga gcggcacaat gctatcagga aggtcgccct gcccggtcgt ggacgtaacc
    17461 accagaacat acttatcctg atagggcagc cagtcgctta attcaggatc ttcaaatacc
    17521 gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt cttccgccac taacagcgaa
    17581 tttccgtaca tggtgccgac aaaaatacca atttccgcca taccgttttc tccctggatt
    17641 aggaacttat ctctccatcc tgacccgatg gcgcactgaa ctcaaccctt tcattttcag
    17701 gaagcagacc gcgccagcca aattgtgata acgcctgcat ccaggtgtcg tccaggcccg
    17761 cgtgaatagt cagccgctcg ccagtaaaag gatgcgtcaa tgacaactga ctggcatgta
    17821 gcattaaccg ctggaggcca aaatgctcag caccgctgcg attctggcgt aaatctccat
    17881 gtttgctatc gccaataatc ggatgacgca aatgggcaag atgtcggcga agctgatgtt
    17941 tgcgtccggt tttcggctcc agttccacca ggccgtagcg cgtggtcggg taacgtccgg
    18001 tcgctaccgg catttctacg gtcgccagac cgcgataatg cgtcactgct ggctgcgggc
    18061 ctttatcttc gcgggcaaat ttatcagcga ttttgtccag ttcttccacc agtggataat
    18121 ccaacaccgc ttcttccatc aaccagccgc gcacaatcgc atggtaacgt ttctggattt
    18181 ggtgctgttc aaactgttgt gccagcagcc gtccggcctc gctggataat cccatcaaca
    18241 acacaccaga agtgggtcgg tccagacgat gagcagtaaa aacatgctgt cctatctggt
    18301 cacgcacggt ttgcatgacc actactttct cgtcgcgatc cagccagcta cggtgaacca
    18361 gccagccgga gggtttattt accgcaacca gccattcatc ctgatagagt atttccagca
    18421 ttagctcgca tcatccgcaa aaagagcatc cagtttttcc aactcagcca gaataagcgc
    18481 gcgttgcgga tggtccgtcg ctagcgccat ttcataatag ggtgcaacag caaaagcgcc
    18541 cggtaacggc tggttgttat ttaacaaatc gtgcattcgc gggatcagcc cccactgcaa
    18601 ccactccagt ggttccatgg tgtccataaa gaacggttgg gtactattaa attgatgcgg
    18661 ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa
    18721 ctggagacga acgcggtcat gagtggtcat aaagcaacct caataagaaa aactgccgcg
    18781 aaggatagca ggaataaaaa aagggagcac tgtattcaca gtgctcccgg ttcgtttcgc
    18841 agcattccag ctacttttgt tgctccctgc tcatccttga caacttttcc tctggccttg
    18901 cggccaatcg ttcatcctga actattgctt cctgctcaca ccaccccgat gtgatacttc
    18961 atcctgaagt gtccctggcc ttcctgaccc accgaatcat cctgaccggt tctcattctc
    19021 catcctggag gtgtccttta acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct
    19081 ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc atcatcctga tgttcacttc
    19141 gttgtctgac tccctgtcga cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg
    19201 cttacagaca atgccttaag gaaaatttca tatgaaagtt aagaattaac aatcaacatt
    19261 ttgattttaa atgaaaataa cgattcttat ctttttaagt attcgcttat ttcctggcga
    19321 tctcctacag agtctgtaag agatctctca caaccagatg gggtatttag attacagaag
    19381 aggcttaagt tgattaagga attccgcaag atttgaggct aaatgcgtcc gtttacgtgt
    19441 tcccagcgtc tctttgcaca cttcaccgga gagattacac accgagatga cctctaactc
    19501 ttcttccagc gtcgcgataa atagcgtggg cggcagtttt agccttttct gagtcaccag
    19561 atgaccaatc agattttcct gcacccgacg gaagtcgtct tcactccagg tttgcagcaa
    19621 tgtcagtttg atatcgccaa actgcgcgtg catatccccg gcaaactgag tcgtataaaa
    19681 ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg
    19741 ttccccggta aacggctggg gttgccagta tacggcatct tcggtagttg aaataatgca
    19801 cggtgaagga acaccgtaca gttcctcact taacggccag cttttatgct cttcatgcca
    19861 cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt gcggtcaaat cgtccactaa
    19921 tttctctctt cacttaaacc agatacactt gtcgtttagt ttatctggtt tatgacggtg
    19981 aaacatgtct tcttatgcaa accatcaggc acttgcgggc ctgactcttg gaaaatcaac
    20041 cgattaccgg gatacctatg acgccagcct actgcaaggc gttccacgca gcctgaatcg
    20101 cgacccgctg ggtctgaaag cggataacct gccttttcac ggtacggata tctggacgct
    20161 gtatgaactt tcctggctga atgcgaaagg tttgccgcag gtcgctgtcg gtcatgttga
    20221 acttgattac accagcgtaa atctgattga gtcgaagagt tttaagctct atctcaacag
    20281 ttttaaccag acgcgtttta ataactggga tgaggtgcgc cagacgctgg agcgcgactt
    20341 aagcacttgc gctcagggta agattagcgt ggcgttatat cgtcttgatg aactggaagg
    20401 ccagccgata ggtcatttca atggcacttg cattgatgac caggatatca ctatcgataa
    20461 ctatgaattc actactgact atctggagaa tgccaccagc ggtgaaaaag tagtggaaga
    20521 gacgctcgtc agccacctgc tgaaatcaaa ctgcctgatc acccatcaac cagattgggg
    20581 ttcgatccaa attcagtatc gtggacgcca aattgacaga gaaaaactgc tgcgttacct
    20641 ggtctcattc cgtcatcaca acgagttcca cgaacagtgc gtggaacgca tctttaatga
    20701 cctgttacgc ttctgccagc cagagaaatt gagcgtttac gcacgttata cccgtcgtgg
    20761 cggtctggac attaacccgt ggcgcagtaa tagcgatttt gtcccatcga ccacaagact
    20821 ggttcggcaa taaatttttt ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa
    20881 ggtcatcagg cagggctatt gtaatcaaag ggaatgacga tattcgtccc ataaggagtt
    20941 tttcttgatt acacatatta gcccgcttgg ctccatggat atgttgtcgc agctggaagt
    21001 ggatatgctt aaacgcaccg ccagcagcga cctctatcaa ctgtttcgca actgttcact
    21061 tgccgtactg aactccggta gtttgaccga taacagcaaa gaattgctgt ctcgttttga
    21121 aaatttcgat attaacgtcc tgcgccgtga acgcggcgta aagctggaac tgattaatcc
    21181 cccggaagag gcttttgtcg atgggcgaat tattcgcgct ttgcaggcca acttgttcgc
    21241 ggttctgcga gacattctct tcgtttacgg gcaaatccat aataccgttc gttttcccaa
    21301 cctgaatctc gacaactccg tccacatcac taacctggtc ttttccatct tgcgtaacgc
    21361 tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc tgctggggcg gtcactcaat
    21421 taacgaaaac gagtatttgt atgcccgtcg cgtcgggaac cagctgggcc tgcgtgagct
    21481 gaatatctgc accggctgtg gtccaggagc gatggaagcg ccgatgaaag gtgctgcggt
    21541 cggacacgcg cagcagcgtt acaaagacag tcgttttatt ggtatgacag agccgtcgat
    21601 tatcgccgct gaaccgccta acccgctggt caacgaattg atcatcatgc cggatatcga
    21661 aaaacgtctg gaagcgtttg tccgtatcgc tcacggcatc attatcttcc ctggcggtgt
    21721 gggtacggca gaagagttgc tgtatttgct gggaatttta atgaacccgg ccaacaaaga
    21781 tcaggtttta ccattgatcc tcaccggccc gaaagagagc gccgactact tccgcgtact
    21841 ggacgagttt gtcgtacata cgctgggcga aaacgcgcgc cgccattacc gcatcatcat
    21901 tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa
    21961 tcgccgtgat acaggcgatg cctacagctt taactggtca atgcgcattg cgccagattt
    22021 gcaaatgccg tttgagccgt ctcacgagaa tatggctaat ctgaagcttt acccggatca
    22081 acctgttgaa gtgctggctg ccgacctgcg ccgtgcgttc tccggtattg tggcgggtaa
    22141 cgtaaaagaa gtcggtattc gcgccattga agagtttggt ccttacaaaa tcaacggcga
    22201 taaagagatt atgcgtcgta tggacgacct gctacagggt tttgttgccc agcatcgtat
    22261 gaagttgcca ggctcagcct acatcccttg ctacgaaatc tgcacgtaat ctcccctcc
//
LOCUS       DAJTYG010000061        21497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTYG010000061 DAJTYG010000000
VERSION     DAJTYG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.061"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(27..2096)
                     /gene="glyS"
                     /locus_tag="OO568_002513"
     CDS             complement(27..2096)
                     /gene="glyS"
                     /locus_tag="OO568_002513"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6641830.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2106..3017)
                     /gene="glyQ"
                     /locus_tag="OO568_002514"
     CDS             complement(2106..3017)
                     /gene="glyQ"
                     /locus_tag="OO568_002514"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6641831.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEMEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3112..3411)
                     /locus_tag="OO568_002515"
     CDS             complement(3112..3411)
                     /locus_tag="OO568_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6641832.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3586..4581
                     /gene="wecH"
                     /locus_tag="OO568_002516"
     CDS             3586..4581
                     /gene="wecH"
                     /locus_tag="OO568_002516"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6641833.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4623..5060)
                     /locus_tag="OO568_002517"
     CDS             complement(4623..5060)
                     /locus_tag="OO568_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR6641834.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5106..5447)
                     /locus_tag="OO568_002518"
     CDS             complement(5106..5447)
                     /locus_tag="OO568_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641835.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5717..7069)
                     /gene="xylB"
                     /locus_tag="OO568_002519"
     CDS             complement(5717..7069)
                     /gene="xylB"
                     /locus_tag="OO568_002519"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6641836.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMLALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGMWGQHWAQQGWRRLRRIQRNRSLSCCRSYR
                     "
     gene            complement(7141..8463)
                     /gene="xylA"
                     /locus_tag="OO568_002520"
                     /pseudo
     CDS             complement(7141..8463)
                     /gene="xylA"
                     /locus_tag="OO568_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8829..9821
                     /gene="xylF"
                     /locus_tag="OO568_002521"
     CDS             8829..9821
                     /gene="xylF"
                     /locus_tag="OO568_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6641837.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKEKEL"
     gene            9899..11440
                     /gene="xylG"
                     /locus_tag="OO568_002522"
     CDS             9899..11440
                     /gene="xylG"
                     /locus_tag="OO568_002522"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR6641838.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11418..12599
                     /gene="xylH"
                     /locus_tag="OO568_002523"
     CDS             11418..12599
                     /gene="xylH"
                     /locus_tag="OO568_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR6641839.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12677..13207
                     /locus_tag="OO568_002524"
     CDS             12677..13207
                     /locus_tag="OO568_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6641840.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13319..13855
                     /locus_tag="OO568_002525"
     CDS             13319..13855
                     /locus_tag="OO568_002525"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6641841.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13963..14787)
                     /locus_tag="OO568_002526"
     CDS             complement(13963..14787)
                     /locus_tag="OO568_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR6641842.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15107..17137
                     /gene="malS"
                     /locus_tag="OO568_002527"
                     /pseudo
     CDS             15107..17137
                     /gene="malS"
                     /locus_tag="OO568_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17315..18568
                     /gene="avtA"
                     /locus_tag="OO568_002528"
     CDS             17315..18568
                     /gene="avtA"
                     /locus_tag="OO568_002528"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR6641843.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRSLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18719..19192)
                     /locus_tag="OO568_002529"
     CDS             complement(18719..19192)
                     /locus_tag="OO568_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6641844.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19294..20142)
                     /gene="yiaJ"
                     /locus_tag="OO568_002530"
     CDS             complement(19294..20142)
                     /gene="yiaJ"
                     /locus_tag="OO568_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR6641845.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTHNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGALT"
     gene            20343..21341
                     /gene="yiaK"
                     /locus_tag="OO568_002531"
     CDS             20343..21341
                     /gene="yiaK"
                     /locus_tag="OO568_002531"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR6641846.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21353..>21497
                     /locus_tag="OO568_002532"
     CDS             21353..>21497
                     /locus_tag="OO568_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6641847.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5404 a         5483 c         5277 g         5333 t
ORIGIN      
        1 tggcaggctt tttaataacg gcgttattac tgcaacagcg aaatatccgc aacgcgcagg
       61 aacagttcgc gcagtttctc cagcatggtc agacggttga gacgcaattc tttgtcatca
      121 accatgacca tcactttatc gaagaaagca tcaaccggct cacgcagctc tgccagttcg
      181 accagcgcat cctggtaacg accttccgca aagtacggct ccagcttgtc acgtagcacc
      241 acaacctgca tcgccagttt aatttcttcc ggctctttca gggtagaggc attcacgcga
      301 tcgctcagca cttcgtcaga tttcgccagg atgttagata cacgtttgtt cgccgccgcc
      361 agtgctgcag ctgcttccag ggtacggaag tgcgataccg ctttcatgcg ggcatcgaaa
      421 tcagccggac gagtcggacg acgcgccagt accgcctgga tggtgtcaac ggtataacct
      481 tcgtcctgat accaggcgcg gaagcgaccg agcataaagt cgataacatc atcaactacg
      541 ttggcattag tcagcttatc gccatacaga cgcaccgctt cttcggtcag cgtttgcaga
      601 tcaaggttga ggttcttctc aacgataatt cgcagcacgc caagtgcggc acgacgcagc
      661 gcaaacgggt ctttgtcgcc tttcggatgc tgaccgatac cgaagatacc cgccagagta
      721 tccatcttat cagcaatcgc cagcgcacaa gctaccgggt tggacggcag gtcatcaccg
      781 gcaaagcgcg gctgatactg ctcattcagc gcgacggcta cgtcttccgc ttcgccatcg
      841 tgacgcgcgt agtgcatccc cataacgcct tgggtgtcgg tgaactcgaa gaccatgttg
      901 gtcatcaggt cacacttaga cagcaggccc gcacgggttg cgtggttaac gtcagcgcca
      961 atctgttcgg cgatccagcc agccagcgcc tggatgcggt cagtcttgtc acgcagcgta
     1021 cccagctgtt gctggaacag cacggtttgc aggcgcggca ggttatcttc cagacgtttt
     1081 ttgcggtcgg tgttgaagaa gaactcggca tccgccagac gtggacgaac gactttctcg
     1141 ttaccagaga taatttgctg cggatctttc gattcgatgt tggcaacgaa gataaagttc
     1201 ggcagcagtt tgccgtcgtt cgcatacacc gggaagtact tctggtcacc tttcatggtg
     1261 taaaccaggg cttcagccgg caccgcgagg aatttctctt cgaattttgc ggtcagaacg
     1321 accggccact ccaccagcga agccacttct tccagcaggc tttcgcttaa gtcagcgtta
     1381 ccgccaatct tacgcgctgc ttcttcggca tcggctttaa tcttcgcttt acgttcttcg
     1441 taatcggcga tgactttccc gcgctcacgc agaatttccg gatactgatc ggcgttgtcg
     1501 atggtgaatt ccggttcgcc cataaagcgg tggccgcgaa tcacgcgatc ggactgaatg
     1561 cccagaatgg ttgccggaat gactttgtcg cccagcagca gggtcacggt gtgcaccgga
     1621 cgcacgaagt gcacgtcgct tgcaccccag cgcatcagtt tcgggatcgg cagtttcgcc
     1681 agcgaagtcg caaccatatt cggcagcagt gcttcggtgc tttcgccctt cacatgggcg
     1741 cgatacagca gccattcgcc tttatcggta gtcagacgct cagcctggtc aacggtaata
     1801 ccacaaccac gcgcccaacc ttctgccgct ttgctcggtt ttccttcagc gtcgaacgcc
     1861 tgggcaatcg ccgggccgcg tttttcgatt tcacgatctg gttgtgcttc cgccaggtta
     1921 gctactttca gcgccagacg acgcggagca gcaaaccatt gaacggtgcc gtgtgcgagg
     1981 cctgcgttat ccagctccgc agtaaagttc gcagcaaagg actctgccag gctgcgcagt
     2041 gcttttggtg gcagctcttc agtgccgatt tccaccagaa aagttttctc agacatagcc
     2101 gcctcttact tatctttgtt gcacatcggg aagccgaggg cttcacggga agcgtaatat
     2161 gcttctgcca ctgctttggt cagggtgcga atgcgcagaa tgtagcgctg acgctcggtg
     2221 acggagatgg ctttacgcgc atccagcagg ttgaagctgt gggcggcttt cagaatacgc
     2281 tcgtaggctg gcagcggcag cggattttcc agcgccagca gctgctgcgc ttctttctcg
     2341 tactgctcaa agcaggtgaa caggaagtcc acatccgcgt attcgaagtt gtaagtggac
     2401 tgctccattt cgttctgatg gaacacgtcg ccgtaggtgg ttttacccag cgggccgtcg
     2461 ctccagacta ggtcgtaaac gctgtctacg ccctgaatgt acatggccag acgttccaga
     2521 ccgtaggtga tctcgccggt aaccggttta cactccagac caccaacctg ctggaagtaa
     2581 gtgaactgcg tcacttccat gccgtttagc cacacttccc agcccagtcc ccaggccccc
     2641 agagtcgggt tttcccagtt atcttccacg aaacggatgt cgtgaatagt cgggtccatg
     2701 cccagctctt tcagagaacc gaggtacagc tcctgaatat tgtccggcga tggcttaatg
     2761 accacctgga actgatagta gtgctgtaaa cggttggggt tttcgccgta gcgaccatcg
     2821 gtcgggcgac gagaaggctg aacataagca gccgccatcg gttctggccc cagcgcgcgc
     2881 agacaggtca ttgggtgaga ggttcccgcg ccgacttcca tgtccaatgg ttgaacaatg
     2941 gtgcagccct ggcgagccca gtaatcctgt aaggtcagga tcaagccctg gaaggtcctg
     3001 gtatcaaact tttgcatatt atttcgtgct ggatacgtgt ggatttaaag gaagggatca
     3061 gtatacccgc tggatggaag atatacagta cgaaacggga aaaagcaggg cttaacgcat
     3121 ggaaagatgc aaaaaatggc catctgcgtc aaaagaacag acaaactgct ctttacggaa
     3181 ggtataaccg cgcatttcat agcttccttg gaactgttcg aaggcggtga cgtcgatttt
     3241 ttgctcgccg gtgttataac gttttgccgc ctgatccttg cataacgctt ccatattcaa
     3301 cgaacgcaga ggatcaacct ttacccgttg tgctttctgc acgggtgacg gtgtagaaca
     3361 acccactagc accataagcg ccattgccgg aaagaaagcg ttcatcatca ttaaattacc
     3421 gtctggtttg caggtgagtc ttattattta tatcggtata gtggctgata tcttacgaat
     3481 ttccttaagc ctctataggc tgtagtgata aaactcagat tattccagtg cgccaaccaa
     3541 atgatttatc ggtcagactt tttttaacca gatacagaga cacagatgca gcccaaaatt
     3601 tactggattg ataacctgcg agggatagcg tgtttaatgg tggtgatgat tcacaccact
     3661 acctggtatg tgaccaatgc tcatagtgtt agccccgtca catgggatat cgccaatgtt
     3721 ctgaattctg cctctcgtgt cagcgtgccg ctatttttca tgatttccgg ctatctcttt
     3781 tttggcgaac gcagcgccca gccgcgccat ttcttgcgta tcggcttatg tctgattttt
     3841 tatagcgcaa tcgcactgct ctacattgcg ctgtttacct ccatcaatat ggagttagcg
     3901 ctgaaaaacc tgctgcaaaa gccagtgttt taccacttgt ggtttttctt cgcgattgcg
     3961 gtgatttatc tggtttcacc gctgattcag gtgaagaacg tcggcggaaa aatgttgctg
     4021 gtgctaatgg tggtgattgg cattatcgct aacccaaaca cagtgccgca gaaaattgac
     4081 ggttttgaat ggctgccaat taacttatat atcaatggcg atacttttta ctacattctg
     4141 tatggcatgt tgggccgcgc tatagggatg atggacacac agcataaagc actgtcgtgg
     4201 gtgagcgccg cgctgtttgc gacgggggtt tttattatct ctcgcgggac attatatgaa
     4261 ttgcagtggc gcggaaattt tgccgatacc tggtatcttt actgtgggcc gatggttttt
     4321 atctgcgcaa tcgcgctatt gactctggtt aaaaacacgc tggatacgcg taccattcgc
     4381 ggacttggct taatctcccg ccattcattg ggtatatacg gattccacgc cttgattatc
     4441 catgcgctgc gcacccgggg aattgagctt aaaaattggc caatactgga tattatttgg
     4501 atcttttgcg cgacgttggc agcgagtttg ttactttcta tgctggtaca acgaatcgac
     4561 agaaacagat tagtgagtta agtaaaagcc cgttcacatt ggactgaccg ggcttacgtg
     4621 aattattcgc tgtattcttc ctgggtaacc tgtgtttctt ttggcgggtt tattccggca
     4681 tcacgaatat tcttctgcac cgctactgca ccaaacaagc ttaagaagaa agccagtcca
     4741 taaaaacctt tttcactgag taataatgtc gcattccaca gacctaccat cagtagagca
     4801 acagagataa taaagacagt caggcaggtc atataataaa tggaagtggt cggtatgcct
     4861 tcatacttgt cccgaacggt cttttgataa gacgcagcag aaaacagtcc taacaccagt
     4921 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacagg
     4981 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
     5041 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
     5101 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
     5161 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
     5221 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
     5281 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga
     5341 aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag ataaaccagc
     5401 gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac gatctccata
     5461 tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat gccattaaca
     5521 aatgattttc agaataaatt catactaaat attaattaat tagtgagata tatagatgtg
     5581 aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc
     5641 tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg ctgcgcatct
     5701 ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga tttctctgga
     5761 ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc ccccccgtac
     5821 ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac tcactacgcg
     5881 ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc acgacatcca
     5941 tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt tcattggggc
     6001 catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg tgtggtgtac
     6061 gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca gcctgttgag
     6121 ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc caatccagac
     6181 acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc gcatggcaaa
     6241 agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca aaatagaccc
     6301 ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca ccaactgcac
     6361 cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc cacgctttcg
     6421 caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg agcatctggt
     6481 cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt gcgacatcca
     6541 gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc atacgcagac
     6601 gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc ggctcatgcc
     6661 gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg ccggtaatca
     6721 ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag cgcccgtcgt
     6781 tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt gctccatgca
     6841 tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg cccagagctt
     6901 tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac caaagtggat
     6961 gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc tgctcgttga
     7021 gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata tcgatcgttc
     7081 cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca cagttagccg
     7141 ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc gaccactctg
     7201 atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca gtgacatttg
     7261 gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct gcgcgatgcg
     7321 tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca gtgccatcgt
     7381 atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt gacgacgtac
     7441 tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa tttcatacat
     7501 caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc ccagttgcgc
     7561 atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg tggctatttc
     7621 atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca gtttaatctc
     7681 tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt aatcatattg
     7741 atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga agccgatttt
     7801 atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct cctgacgcaa
     7861 gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat agttttcacc
     7921 gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc agctgaagac
     7981 ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa agcagttagc
     8041 ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga catcaaccat
     8101 ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa catccacatc
     8161 gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga catctgcttt
     8221 acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa acgcccccac
     8281 accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg caaaacgcaa
     8341 gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac ggaatgctaa
     8401 cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa aataggcttg
     8461 catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt aatattgggc
     8521 actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa ttcacaagtg
     8581 tgcgctcgct cgcaaaataa aatgggatga tgaaactggg taattcctcg aagataaaaa
     8641 tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa gaaataaacc
     8701 aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag ttgctaaaaa
     8761 ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac tacagaaggc
     8821 cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt ctgcttacca
     8881 acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat ctccgtcttg
     8941 aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc ggtgcgaaag
     9001 tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt gaaaacatga
     9061 ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta ttaagtaacg
     9121 ttgtaaaaga agccaaacaa gaaggtatta aagtattagc ttacgaccgt atgattaacg
     9181 atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa ctgcaggcaa
     9241 aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc tcgccggtag
     9301 ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct tacgttgatt
     9361 ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg gaaaacgcat
     9421 tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct gtagttgcct
     9481 caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt ttatcaggga
     9541 aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt gctgccggta
     9601 cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc gcagaaattg
     9661 ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat aatggcctga
     9721 aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac atcaaagata
     9781 cggtaattaa agacggattc cacaaagaga aagagctgta agcgttacgc cccggcgcgg
     9841 agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg gagtcgttat
     9901 gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga aggcgattga
     9961 taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg aaaatgggtc
    10021 tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct cctacgaagg
    10081 cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata ccgaacgcaa
    10141 aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg tgctggaaaa
    10201 tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc tgatgacgct
    10261 acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata cccgcgttgg
    10321 cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta ataaacaggt
    10381 gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa cgtcggtttt
    10441 actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata tttcgcacaa
    10501 actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg gacagcacat
    10561 tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga tggtcgggcg
    10621 agagttaacc gcgctttacc ctaatgaacc acataccacc ggagatgaaa tattacgtat
    10681 tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta atgatgtctc
    10741 gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg ccggacgtac
    10801 cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa aaatttatat
    10861 tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcgcccagg ggattgcgat
    10921 ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg gtaaaaatat
    10981 taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg acgcggcaga
    11041 gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt cccccgacct
    11101 tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc gctgtctgtt
    11161 acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata ttggcgcgaa
    11221 atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg ttattgtcat
    11281 ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga tgcatgaagg
    11341 gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga tggaagccgc
    11401 attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg gccgtaccga
    11461 catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg atgattgcag
    11521 ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac ttaagcgccc
    11581 gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg gtaggaatgg
    11641 tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg gggctattag
    11701 gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt accatcattg
    11761 tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc gcgtaccgca
    11821 aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc atactcattg
    11881 gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa attgggcaaa
    11941 gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg gcttttgttg
    12001 gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct ccggcctcta
    12061 ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca atctggctgt
    12121 tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg ttactcggcg
    12181 gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc ggcggcaatc
    12241 tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc gtgttcgcga
    12301 ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt ggcgctggtt
    12361 caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg attggcggca
    12421 ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca tttatcatgg
    12481 cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag tatatcgtta
    12541 aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc cgttcttgat
    12601 tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt ttgcatgatt
    12661 cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt tcaatgccaa
    12721 taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg catcacaatc
    12781 agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa tcaaggactg
    12841 gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag cgctggctga
    12901 tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa gttacccacc
    12961 cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt tgcatttaaa
    13021 agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg gcaaacgttg
    13081 ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt atcgcggagt
    13141 ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa atcggctggc
    13201 agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg acgcccgagc
    13261 gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa aattatgcgt
    13321 gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc tttcttcggt
    13381 cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc gattattaga
    13441 taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca ttgaacggcg
    13501 ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc attacattcg
    13561 taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg ggatctcgcg
    13621 ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg caatgattca
    13681 tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt cgatcaatga
    13741 gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt ttaaaaaagc
    13801 atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt tgtagcgatg
    13861 atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc gagcaaaagc
    13921 cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat gcgccgcgat
    13981 taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct tccctttggt
    14041 cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat ccgctttacg
    14101 cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca ggttagtgac
    14161 ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca ctttacccgg
    14221 cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat tgttgcgcgc
    14281 cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca tactggtagg
    14341 gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat taccagacca
    14401 cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag gtgaccattg
    14461 gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg cattttggct
    14521 ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc cggaagggta
    14581 ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact cttgtttact
    14641 gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg ttgttgtgtg
    14701 cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa tcatcgcccc
    14761 atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat cattccaaca
    14821 ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa tagctgtcaa
    14881 gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga ttcagattgg
    14941 cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt gcgcgcgatc
    15001 gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt gtctggctta
    15061 tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga aactcgccgc
    15121 ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt ctccggggtt
    15181 ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt tgcccaaagg
    15241 tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag atgcgataaa
    15301 actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat ggcgattgtt
    15361 cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa cattgatgat
    15421 ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc cgaaatggga
    15481 tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg ccgtacggga
    15541 ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac aacccgctgc
    15601 taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg cccctttcga
    15661 ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg gcgatcccag
    15721 taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca cttttcacgg
    15781 cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg gcgttaatgc
    15841 tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggcg gtacaaaagg
    15901 cgatttcccg cattatgcct accacggtta ttacacacag gactggacga atcttgatgc
    15961 caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc agcgcggtat
    16021 tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg cggatatgca
    16081 ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa cgctgggtga
    16141 acgctggagc gactggaaac ctgccgccgg gcaaacctgg catagcttta acgattacat
    16201 taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga tcagaacgga
    16261 tatcggcgat tacgacaatc ctggattcga cgatctcact atgtcgctgg cctttttgcc
    16321 ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata aaaacaaaac
    16381 ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa cccactggtt
    16441 aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg ccaaacatgt
    16501 tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc gcgaatggaa
    16561 aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg gtgaagccta
    16621 gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga tgatcaattt
    16681 cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg atacgacctg
    16741 gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct cgtcgcatga
    16801 tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat tagcgccagg
    16861 cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta caggttctga
    16921 tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta aatctgccgc
    16981 caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg caattggcgc
    17041 gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg agcatggcga
    17101 cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc ccgttcgtca
    17161 gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt tgcgggataa
    17221 agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg ttattgttac
    17281 cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt ttggtgacaa
    17341 atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg gtttacgcac
    17401 gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa tgcaggacta
    17461 cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg cactgtgtaa
    17521 ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa tgctgcgcga
    17581 gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca gccagagcgc
    17641 gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg tcaaaaaagt
    17701 gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg aagaagatct
    17761 gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta aataccacgt
    17821 cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct cccggccgac
    17881 gaatccaaca ggcaatgtga ttactgacga agagttgctg aagcttgacg cgctggcgaa
    17941 tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc cgggtatcat
    18001 cttcagtgaa gcgcgcccgc tatggaatcc gaatatcgta ctgtgcatga gtctttccaa
    18061 gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa tcatcactgc
    18121 catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc cggcgatgat
    18181 gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca tcaaaccgtt
    18241 ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac cggaaaatcg
    18301 cagcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta aggatttgcc
    18361 cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga tggtgccggg
    18421 gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat gtatgcgcat
    18481 gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg cggaagagat
    18541 agaaagagct tgggcagaaa gtcactaaaa attgcctgat gcgctacgct tatcaggcct
    18601 acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa ggcgtttacg
    18661 ccgcatctgg cataaacaaa gcgcactatt ccggcgttga gaaacgccgg aaaacgtttc
    18721 aaacaggctg ctgctgtagc cgatgccgct gcactttctc gacatccatg cactgcaacg
    18781 cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag cacagatcgc
    18841 acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca aacgggcaag
    18901 ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata tgcccatgtt
    18961 cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca cactgatgac
    19021 aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa ataaactcgt
    19081 ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa gccacttcac
    19141 aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc atgatcattc
    19201 ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga tccaacgaag
    19261 gaaatgttaa agttttggca cttgtccaaa gcgttatgtg agcgcgccca ggtcatccct
    19321 gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg gtttcaggag
    19381 atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca cggcgtacgg
    19441 cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga gttcgttttc
    19501 ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga acatcgcggg
    19561 cagctcggta atggtattgt gggttaacgg ctggatctca tgctgatggt tttcccagta
    19621 tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg cggaacagta
    19681 gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg tgggttccag
    19741 cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg tttcaccagt
    19801 ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca gcgaagacag
    19861 cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg cggcgggcgc
    19921 ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg tactcttatt
    19981 taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt tgctcaaaat
    20041 ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct ctttttcctg
    20101 cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc gtcctgatgg
    20161 tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca cttgattttt
    20221 atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat atatttgaaa
    20281 tctagtttcg catattgaaa ttttaagcca aaaaagcgat caaaaaaaca aggaagccta
    20341 ggatgaaagt gacatttgag cagttaaaag cagcctttaa tcgggtctta atttcacgcg
    20401 gcgttgacag cgaaacggct gacgcctgtg cagagatgtt cgcccgcacc accgaatccg
    20461 gcgtttattc tcacggcgtt aatcgtttcc ctcgtttcat tcaacaactg gaaaacggcg
    20521 atatcattcc tgatgcccaa cccaaacgta taaccagcct cggcgcaatt gaacagtggg
    20581 atgcccagcg ttcgatcggc aacctgacgg cgaaaaagat gatggatcgc gccattgaac
    20641 tggctgccga tcacggtatt ggtctggtgg cacttcgtaa tgccaaccac tggatgcgcg
    20701 gcggcagcta cggctggcag gcggcggaaa aaggctatat tggcatttgc tggaccaact
    20761 ccatcgccgt aatgccgccg tggggcgcaa aagagtgtcg catcggcact aacccgctga
    20821 tcgtcgccat tccttccacg ccgatcacca tggtcgatat gtcgatgtcg atgttctctt
    20881 acggcatgtt agaagttaac cgcctggcag gccgtcagct cccggtcgat ggtggctttg
    20941 atgatgaggg caatttgacc aaagaacctg gcgttatcga gaagaatcgc cgcattttgc
    21001 cgatgggcta ctggaaaggt tctggcatgt cgattgtgct ggatatgatc gctactctcc
    21061 tttccgacgg cgcatcggtt gccgaagtca cccaggacaa cagcgacgaa tacggcattt
    21121 cacaaatttt tattgccatt gaagtggaca agcttatcga cggtcccacc cgcgatgcca
    21181 agctgcaacg tatcatggat tacgttacta ccgctgaacg cgctgacgaa aaccaggcca
    21241 tccgcttacc cggccacgaa tttactaccc tgctggccga aaaccgccgt aacggcatca
    21301 ctgttgatga cagcgtgtgg gccaaaatcc aggcgttatg aggagataag tcatgatttt
    21361 tggacatatc gcgcagccca atccgtgccg tttgcccgcc gccattgaaa aggcgctcga
    21421 ttttctgcgc gctaccaact tcaacgtcct ggagccgggc gttgtcgaaa tcgacggcaa
    21481 gaatatttat gcgcaaa
//
LOCUS       DAJTYG010000062        21366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTYG010000062 DAJTYG010000000
VERSION     DAJTYG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.062"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..755
                     /locus_tag="OO568_002533"
     CDS             <1..755
                     /locus_tag="OO568_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6641848.1"
                     /translation="SGIGLAIVEELLAQGANVQMVDIHGGDGQYEGHKGYQFWPTDIS
                     STKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAPAGQYELNEAAFEKMVNIN
                     QKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSCYAATKAALNSFTRSWSKE
                     LGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQLREGYTKNAIPIGRAGRL
                     AEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            765..1172
                     /locus_tag="OO568_002534"
     CDS             765..1172
                     /locus_tag="OO568_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6641849.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLASIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            1172..1666
                     /locus_tag="OO568_002535"
     CDS             1172..1666
                     /locus_tag="OO568_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR6641850.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            1732..2529
                     /locus_tag="OO568_002536"
     CDS             1732..2529
                     /locus_tag="OO568_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6641851.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2540..3328
                     /locus_tag="OO568_002537"
     CDS             2540..3328
                     /locus_tag="OO568_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6641852.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3380..4609
                     /gene="sorE"
                     /locus_tag="OO568_002538"
     CDS             3380..4609
                     /gene="sorE"
                     /locus_tag="OO568_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR6641853.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            4991..5680
                     /gene="pepE"
                     /locus_tag="OO568_002539"
     CDS             4991..5680
                     /gene="pepE"
                     /locus_tag="OO568_002539"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6641854.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(5771..7402)
                     /locus_tag="OO568_002540"
     CDS             complement(5771..7402)
                     /locus_tag="OO568_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6641855.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7622..11305)
                     /gene="metH"
                     /locus_tag="OO568_002541"
     CDS             complement(7622..11305)
                     /gene="metH"
                     /locus_tag="OO568_002541"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6641856.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMVDYQMESLSAE
                     INFAAAKLARARADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11505..12329
                     /gene="iclR"
                     /locus_tag="OO568_002542"
     CDS             11505..12329
                     /gene="iclR"
                     /locus_tag="OO568_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6641857.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            12646..14830
                     /gene="espL4"
                     /locus_tag="OO568_002543"
                     /pseudo
     CDS             12646..14830
                     /gene="espL4"
                     /locus_tag="OO568_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(14799..16535)
                     /gene="aceK"
                     /locus_tag="OO568_002544"
     CDS             complement(14799..16535)
                     /gene="aceK"
                     /locus_tag="OO568_002544"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCR6641858.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(16719..18023)
                     /gene="aceA"
                     /locus_tag="OO568_002545"
     CDS             complement(16719..18023)
                     /gene="aceA"
                     /locus_tag="OO568_002545"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR6641859.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(18053..19654)
                     /gene="aceB"
                     /locus_tag="OO568_002546"
     CDS             complement(18053..19654)
                     /gene="aceB"
                     /locus_tag="OO568_002546"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCR6641860.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGCGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(19923..20852)
                     /gene="metA"
                     /locus_tag="OO568_002547"
     CDS             complement(19923..20852)
                     /gene="metA"
                     /locus_tag="OO568_002547"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6641861.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            21009..21299
                     /locus_tag="OO568_002548"
     CDS             21009..21299
                     /locus_tag="OO568_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6641862.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5243 a         5499 c         5412 g         5212 t
ORIGIN      
        1 catccggtat tggtctggcg attgtagagg aattattagc acaaggcgcg aatgtacaga
       61 tggtcgatat tcacggtggc gatggtcaat atgaaggtca taaaggctat cagttttggc
      121 cgaccgatat ttctagcaca aaagaggtaa atcatacggt agcggaaatt atccagcgtt
      181 ttggtcgcat cgacggtctg gtcaataacg ccggggtcaa tttcccgcgt ctgctggtcg
      241 atgagaaagc gcctgccggg cagtatgaac tcaacgaagc tgcattcgaa aaaatggtca
      301 atatcaacca gaaaggtgtt tttctgatgt cgcaggcggt ggcgcgacag atggtcaaac
      361 aacatgatgg cgtgattgtg aatgtttcct cagaaagtgg gctggaaggc tcagaaggac
      421 aaagctgtta cgccgctacc aaagccgcgc tcaatagctt cacgcgctcc tggtcgaaag
      481 agctgggtaa acacggtatc cgtgtggtcg gtatcgcgcc cgggattctg gaaaaaaccg
      541 ggctacgcac gccggaatat gaagaggcgc tggcgtggac gcgcaatatc accgtcgagc
      601 agctgcgtga aggctatacc aaaaacgcca ttcctattgg gcgcgccgga agattagcag
      661 aagtagctga ttttgtttgt tatctgttgt ctgaacgcgc cagctacatc accggagtaa
      721 ccactaacat tgcgggcggc aaaacgcgcg gctaaggagg ctttatggtc aacgccattt
      781 tttgtgccca cggcaaatta gcttgcgcca tgctggaatc ggttcagatg gtttacggca
      841 acgccaatgt tgaagccgtt gaatttgtgc ccggtgaaaa cgccggagac attgcggtaa
      901 agctggaaaa gttagcaagc attcacaatc aagatgagtg gctgattgcc gtggatttac
      961 agtgcggcag cccgtggaat gcggcagcaa tgcttgcgat gcgcaatcct cgtttgcggg
     1021 taatcagcgg cctttctctg ccgctggcgc tcgaactggt tgataaccag gacagcatga
     1081 atgttgatga actgtgtgaa cacctgacgc aaatcgcaaa gcaaacctgc gttgtctgga
     1141 aacaactggc aacgacagag gaggatttct gatgaacatt acgctcgccc gcattgatga
     1201 ccgtctgatc cacggccagg tcactaccgt ctggtcgaaa gtggctaacg cccagcgcat
     1261 tattatctgc aatgacgaag tttataacga tgaagtgcgc cggacattat tgcgtcaggc
     1321 tgcgccaccg ggaatgaagg ttaacgtggt caatattgaa aaagccgtcg cggtttatca
     1381 caacccgcaa tatcaggacg aaaccgtttt ttatcttttc actcgcccac aggatgcctt
     1441 agcgatggtt cgccaggggg taaaaatagg caccctgaat attggcggca tggcctggcg
     1501 gcccggtaaa aaacagttaa ccaaagcggt ttcattagat gatgatgata ttaacgcatt
     1561 tcatgagtta aataatcttg gtgtcatact ggatttacgc gtcgtagcgt cagatccctc
     1621 aatcaatatt atcgacaaaa taaacgaaca gttaatcgca aattaaataa tagcgcctgt
     1681 gtacatatgc tttaacaggc agggatgacg acacttaaaa ggtgacaaat catggaaata
     1741 agtaccctac aaatcattgc tatatttctt ttttcctgta ttgccggaat gggcagcgtg
     1801 ctggatgaat ttcagactca tcgcccgtta attgcctgta cggtgattgg tttaattctc
     1861 ggtgatttaa aaaccggaat aatactcggc ggtacgctgg aattgatagc tctcggctgg
     1921 atgaacgtcg gcgcggcgca atctccggat tctgcactcg ccagcataat ctccgccatt
     1981 ctggttatcg ttggtcagca aagcatcgcc accggaatcg ccatcgcgtt gcctgtggct
     2041 gcggcaggcc aggtgctgac ggtgtttgcc cgtaccatca ccgtagtgtt ccagcacgcg
     2101 gcggataaag cagcagaaga ggcgcggttt cgcactctcg atattctgca tgtctccgcg
     2161 cttggcgtgc aggcgctgcg cgtcgccatt ccggcactga ttgtctcact gttcgtcagc
     2221 gccgatatgg tcagcaatat gttaaacgcc attcccgaat ttgtgacccg aggactacag
     2281 attgctggcg gttttatcgt ggtggtcggt tacgccatgg tgctacgtat gatgggcgtg
     2341 aaatatttga tgcctttctt tttcctcggt ttcctcgcag gtggctacct cgatctcagc
     2401 ctgctggcgt tcggtggcgt cggcgtgatc atggccctgg tctacatcca gctaaatcca
     2461 cagtggcgta aagctgaacc tcaactccag gccaccgcct ccaccgccct tgaccaactt
     2521 gacgattaac ggagccatca tggaacagag aaaaattaca cgcagcgatc tggtgagcat
     2581 gtttctgcgc tccaacctgc aacaggcgtc ctttaacttt gaacgtattc acgggctggg
     2641 cttttgctac gacatgatcc ccgccatcaa gcgactttac ccattaaaag aggatcaggt
     2701 tgcggcgctc aggcgacacc tggtgttctt caataccacg ccagccgtat gtggcccggt
     2761 catcggcgcg gaaattgatg acggtaccat caacggcatc aaagtcggtc tgatgggacc
     2821 attggcagga gttggcgatc cactggtctg gggaacgctg cgcccgatta ccgccgcgct
     2881 cggcgcatct ctggcacttt cgggcaacat tctcggcccg ctgctgttct tctttatttt
     2941 caacgcggtg cgtctggcga tgaagtggta tggcctacag ctcggctttc gcaaaggggt
     3001 gaatatcgtc agcgatatgg gcgggaatgt gctgcaaaaa ctcaccgaag gcgcgtcgat
     3061 tctcggactg tttgtgatgg gcgtgctggt gaccaaatgg acgtcaatca acgtaccgtt
     3121 ggtggtttca caaacgcatg ccgccgatgg ctccaccgtc accatgaccg tgcagaacat
     3181 tctcgaccaa ctttgccctg gtttgctggc gctcggtctg acgctactaa tggttcgtct
     3241 gctcaacaaa aaaattaacc cggtatggct gattttcgcc ctgtttggct tagggattat
     3301 cggcaatgct ctgggcttcc tgtcctgatt cttcgccccg gcacgactgc cgaggccatc
     3361 gctcaacatg aggtggttta tgaaaacaac agctctgcgt ctttatggta aacgtgattt
     3421 acgcctggaa acctttgacc ttcctgaaat gcaggaggat gaaatcctcg caacggtggt
     3481 cactgacagc ctgtgcctct cttcctggaa agaggccaat ctgggtgaaa accataaaaa
     3541 agtacccgac gatgtggcga ccaacccaat catcatcggc cacgaatttt gcggcgatat
     3601 tctggccgtg ggtaaaaagt ggcagcacaa attccagccg ggtcagcgtt atgtgattca
     3661 ggccaacctg caactccccg accgcccgga ttgccccggc tactccttcc cgtgggtagg
     3721 cggcgaagcg acgcatgtgg ttattcccaa cgaggtcatg gaacaagatt gcctgctggc
     3781 atacgacggc gaaacctatt ttgaaggctc actggttgaa ccgctttcct gcgtgattgg
     3841 cgcgttcaac gccaactatc atcttcagga aggtagttat aaccacacga tggggattcg
     3901 cccgcaaggg cgcacgctga tcctcggcgg caccggacca atgggactgt tggcgattga
     3961 ttatgcgctg catggaccag ttaacccgtc gctgctcgtc attaccgata ccgacaacga
     4021 caaattgagt tatgcgcgca agcactatcc atcagaaccg caaacactga ttcattatct
     4081 caatgccacc gatgcagcat ttgatacgtt aatggcgctg agtggcggtc acggcttcga
     4141 tgatattttc gtctttgtgc ctaatgaagg actggtgact ctcgcctctt ccttgctggc
     4201 gacagatggt tgcctgaatt tcttcgccgg accgcaggat aaacatttca gcgcgccaat
     4261 taatttctac gatgtgcatt atgcatttac ccactacgtg ggcacgtcag gcggcaatac
     4321 cgacgacatg cgcgcggcgg tcaaactgat cgaagagaaa aaagtgcagg ccgcaaaagt
     4381 ggtaacacat attcttgggc tgaatgccgc gggcgaaacc acgcttgaat tgcctgccgt
     4441 cggcggcggc aaaaagctgg tgtataccgg gaaatacctg ccgctgacgt cactcacgca
     4501 gattcaggat caagcactgg cggcgattct ggcgcgtcat caggggatct ggtccggtga
     4561 ggcggagcaa tacctgctcg cccatgcaga ggcaatttcc catgattaat cgcgctactt
     4621 aactctgcat gtcgctggct ggtcgcccca gcaacttcgc gaccggatgt gcggctgatt
     4681 gctgaagcag cacaatttgc acgagaagga taaaaaatat cagcgcctta aggggcgctc
     4741 aaattggtgg caaagtacac attgtttatg ccggatgcgg aatgaagcct tattcggtct
     4801 acataatttt gaaaatccaa tatattgcag ttatctggta ggctaggtaa gcgtagcgca
     4861 tcaggcaatg ctgtgtttgt catcagtctc agcgcccggt gcaatacgtg acatcgtgag
     4921 ccatgtcagt aagtggttaa attcgcagtg gtagcatgcc ttccgtgatt tcttactaac
     4981 cggagaagta atggaactgc ttttattgag taactcgacg ctgccgggta aagcctggct
     5041 ggaacatgca ctgccgctaa ttgctgaaca gttgcagggt cgccgctcag cggtgtttat
     5101 ccctttcgct ggcgtaacgc agacctggga tgattacaca gagaaaacgg ctgcggttct
     5161 cgctccgctg ggtgtttctg tcaccggtat tcatagcgtt gtcgatcccg ttgccgcgat
     5221 tgaaaatgcg gaaattgtga ttgtcggcgg cggtaatact ttccagttgc tgaaacagtg
     5281 ccgcgagcgc gggctgctgg ctccaattac tgacgtggtt aaacgtggcg cgctgtatat
     5341 tggctggagc gcaggcgcta accttgcttg cccaaccatt cgtaccacca acgatatgcc
     5401 gattgtcgat ccgcaaggtt tcgatgcgct aaatctgttc ccgttgcaaa tcaacccgca
     5461 cttcaccaac gcgctgccgg aaggccataa aggtgaaacc cgtgagcagc gtattcgcga
     5521 actgctggtc gtcgcgccag aactgacgat tattggtctg ccggaaggta actggatcac
     5581 agtgagtaaa ggtcacgcta cgctgggtgg cccgaacacc acttatgtgt ttaaggctgg
     5641 tgaagaagcg gttccgctgg aagctggtca ccgtttttaa gtgaattgcc agatgcatga
     5701 tccggcatct tgttcatagt gcctgatgcg acgcttgacg cgtcttatca ggcctacagg
     5761 attcacaata ttaatactcg tcccgtcctt catcttcatc cggctgttcc agcacactgt
     5821 aagccaccga acaaaacagc gagttcaggc gctgcatatc tcccagtaag cctaaatgta
     5881 gcgaactggt ttcaatgctc tgcacgtttt gctgatgcag gcgatcgacg tgggcgtggg
     5941 aatagcggcg attaagaatg cgaaaacgat gtttgctgcg acgcaaacga cgagcgctgg
     6001 tgacatcgcc agagaagaac accgacattg ccagctttaa attactgagc aattgctcat
     6061 agagcgcatc cagttccttc aacccatcaa gcgaaaatgc ccgccgtgct gccagcgatt
     6121 tatcggcaat ttcgctgccc atgcgctcga cgatatcgga ggcctgttca aggttgagcg
     6181 acatttcgat gatctccgcc cagcggcgcg actcttcctc cgccaactct tcttttggca
     6241 tccgcgccag atacagctta atggcggtgt agagaacgtt gatatcatct gccagcttac
     6301 gcagctcttt ctcctgccgt ggctcgccgt gcatcacttt attcagccct tccatcatct
     6361 gttccatggc gtcgccaatg cgcagggttt cgcgcgcggc gttcgccaga gcaagcgtgg
     6421 gcgtgtccag cgcgctgaca tccagatgtt taggccgtag ctgggtatcc agttccggtt
     6481 catcgcgaat aatcgttttg caaaaccgtg ccatcgggtc aacaaatggc agcatgacta
     6541 ggcaacgtac aaggttgtag aagacgtgga aatagatcac cagttccgct tttggcaatg
     6601 acagcttccc cattgtctct gccagcaaat ggacaaacgg caggataatc aggctaccca
     6661 ccagcttaaa cagcagacta cccagcgcga cacggcgggc tgcggcattg gcggcactgt
     6721 tgttgagcat cgccagcagg ccggaaccga ggttagctcc aatcaccaga cagagcgcca
     6781 cggggaagga gataatgcct gcagcggtca gagtcgcggt cagcagtaca gcagcaaggc
     6841 tggagtagct gataatggcg aacatcgcgc caatcagcgc atccagcaga atatcgccgg
     6901 tcagcgaggc aaagatcacc tgaacgccgt ttgcctgggt gatcggcgtt acggcctgca
     6961 caatcaactc cagcgccagc aaaatcagcc caagaccaat accgacgcgg cccagttgcc
     7021 cggcgcgtga ctgtttgcgt ccgaggaaaa aaatcacgcc gataaaaata agtaacggtg
     7081 acagccagga taagtcgaag gtgagaatac gcgccattag cgccgtcccg acatctgccc
     7141 ccagcacaat gaccagagcc ggtgcgaggg ctaccagatc ctgagcgacg aacgaggtca
     7201 ccagcatggt ggtggcatta ctgctctgta ccagtgcggt aacgccgatc cccgcgcaaa
     7261 aggcgagcgg ctttttttcg acgctccggc taaggacagt acgcaaacgc gcgccgaaga
     7321 cgcgcattac gccggttcga acaatatgag tcccccagac cagcagggcg acggcagaaa
     7381 gcaggtgaag cagcgttaac acgagttcag gttctcctta tcgttatatg ttcctgaagc
     7441 aattgaatga ccaaaggacc ggaaacacgc tgactggcat ctttactgtg ccttttgaca
     7501 ttcgtttttc cggctaagat tattccagcc tgtttccagt ataagtgttt catattaata
     7561 aagagacagg gcactcattg agcgccctgt ttgttgtaag gaaaagtgac agatttgtga
     7621 atcagtccgc gtcatacccc agattcggtg ccagccagcg ctcaacatcg gagacgctca
     7681 tacctttacg gcgggcatag tcttcaacct gatcgcgctg aatttgcgcc accgcgtagt
     7741 acttgctgtc cggatgactg aaataccaac ccgaaaccga tgcaccaggc cacatagcga
     7801 aagattctgt gagtttcatg ccagtatgtt tttccacttc cagcagctcc cagatggtgg
     7861 ctttttccgt atgttccggg caggccggat agcccggtgc cggacggatg ccctggtagt
     7921 tttcgcggat cagttcttcg ttgctgaggt tctcgttcgg cgcatagccc cagtagactt
     7981 tacgcacacg ctcatggaga tactccgcaa aggcttcggc taaacggtcg gcaagcgctt
     8041 tcaccatgat tttgttgtaa tcatcgtgct gcgcttcaaa ggcatcagcc agtgcgtcct
     8101 cttccagccc gccagtcacg gcaaatgcgc cgatgtaatc tgctttacca gaaagcttcg
     8161 gcgcaacgaa gtcagcgaga cagtagttag cgaatcccgt tttttcggtc tgttgacgca
     8221 gatggtggct gacgttgatc acatgggtgc gcgtttcgtc acggtagatt tcaatgtcat
     8281 cgcccacacg gtttgccggg aacaggccca ccacgccacg cggattcagc gttttctcgg
     8341 cgcttaattt atccagcatg tcgttggcgt ctttaaacag ccgctgcgcc tcaacgccca
     8401 ccacttcatc ttccagaatg cgcggatact tcccggccag cgaccaggtc ataaagaacg
     8461 gtgtccagtc gatgtaatta cgcagcgttt cgatgctggc ttccacttcc tgcacgccga
     8521 gacggtgcgc caccggcggc gtgtaagcct gccagtcaaa tgcgaaatcg ttatcgcgcg
     8581 ccgcttccag cgtgaccggt ggtgtgcgtg gtttcttgcg cccgtgctga atacgcacgg
     8641 tttcgtactc cttgcaggta cgagcgacaa aatcatcacg ctgagtatcg gaaagcagcg
     8701 ccgctaccac accaacggtg cgcgaggcat tctgcacata caccgtaggg ccgctgtagt
     8761 tctgctcgat tttcaccgcc gtgtgcgctt ttgaggtggt cgcgccgcca atcagtaacg
     8821 gaatggtgaa gccctgacgc tccatctctt tcgccacgtt aaccatctcg tccagcgacg
     8881 gcgtgataag ccccgaaagg ccaatcaaat cagcattcac ttctttggcg atacggagaa
     8941 ttttttccgc aggcaccata acgccgagat cgacaatttc gtagttgtta cattgcagca
     9001 ccacaccaac gatattttta ccgatgtcgt ggacatcgcc tttcacggtg gcgatcacca
     9061 tcttgccgtt ggttttaccc tgctctttgc tggcttcaat aaacggttcg agatacgcca
     9121 ctgcctgttt catgacgcgc gccgatttga ccacctgtgg caggaacatt ttgccttcgc
     9181 caaacagatc gccgaccacg ttcatgccgt ccatcaacgg gccttcaatc acttcaatcg
     9241 ggcgcgtagc ctgctggcgg gcttcttcag tatcctgctc gataaactcg gttatgcctt
     9301 tgaccagcga gtattccaga cgtttattca cgtcccacga gcgccactcc gcttgctggg
     9361 cgttagcagt gtcgtccgct ttactgccgc gatatttctc ggcaagctcc agtaaacgct
     9421 cagtgccatc gtcacgacga ttaagaatca catcttccac cgcgtcgcgc agttcagcgg
     9481 gtaggtcgtc gtaaatcgcc agttgcccgg cgttgacgat ccccatatcc atgccattgc
     9541 gaatagcgta gtagaggaac actgcgtgaa tggcttcgcg caccggatcg ttgccacgga
     9601 atgagaaaga aacgttagat acgccgccgg aaatcagcgc gtgcggcagt tcgcgtttga
     9661 tgtcttcaca cgcgccgata aagtcctgcg cgtagttgtt gtgctcttca ataccggtgg
     9721 cgaccgcgaa gatgttcggg tcgaagatga tatcttctgg cgggaagcca acctcttcgg
     9781 tgaggatttt gtacgcccga cggcaaatct cgattttccg tgcgcgagta tcggcctgtc
     9841 cctgttcgtc aaaggccatc accaccactg ccgcaccgta gcgacgcaac aatttcgcgt
     9901 gatggataaa ggcgtcgacg ccctctttca tcgagataga gttaacaatg cctttgccct
     9961 ggatacactt cagacctttt tcaatgacat cccattttga ggagtcgatc ataatcggca
    10021 cgcgagcgat atccggttca ccggcaatca gattgagaaa acgcaccatc gccgcttcgg
    10081 catcgagcat cccttcatcc atgttgatat cgataatctg cgcgccgttt tccacctgct
    10141 gacgcgccac atccagcgcc tcgctgtatt tctcttcttt gatcaggcgc ttgaacttag
    10201 cagagccggt gacgttggtg cgttcaccca cgttcacaaa caggctatcg tcgccaatgt
    10261 tcagcggctc caggccagac aaacggcagg ctacgggaat ttccggcagt ttgcgcggcg
    10321 ctaatccttc tactgcacga ctcatcgctg caatatgttg cggcgtggtc ccgcagcaac
    10381 caccgacgat attgagaaag cccgcttgcg cccattcacg tatctgtttt gccatcgtgt
    10441 cggcgtcgag atcgtattca ccaaaggcgt taggcagccc ggcgttcggg tgggcggtga
    10501 cgtagcattc cgcaatccgt gatagttcct gcacgtactg gcgcagttca tcgggcccca
    10561 gcgcacagtt caggccaaag gtcagcgctt cggcgtggcg caatgagttg taaaatgctt
    10621 cggtggtctg cccggagagc gtgcgcccgg aggcgtcggt gatggtgccg gagatcataa
    10681 tcggcagctc aacgcccagc gcttcaaact ccgttttcac cgcaaagacc gccgctttgg
    10741 cgttaagggt gtcgaaaacg gtttcaatca ggatcagatc cgcaccacct tccaccagcg
    10801 ctttggtgga ttcgcgatag gccgccacca gctggtcaaa agtgatgttg cggaatgccg
    10861 gatcgttgac gtccggagaa atagacgccg tgcggttggt tgggccgaga acaccggcaa
    10921 cgtagcgcgg tttctccggc gtgcgcgcgg tccactcgtc agcacgggcg cgcgccagtt
    10981 tcgccgccgc aaagttgatt tccgccgaca gggattccat ctggtaatcc accatcgcaa
    11041 tggtcgtgga gttgaaggtg ttggtttcga tgatatccgc gcccgcttca aagtaggcgt
    11101 tgtggatagc ggcgatcact tccggtttac tgagtaccag caggtcgttg ttgcctttga
    11161 ggtcgcatgg ccagtcggca aagcgttcac cacgaaaatc ggcttcgttc agtcgatagc
    11221 tctggatcat ggtgcccata ccgccgtcca acaccagaat acgttcattt aactgcgcac
    11281 gcagttgttc cactttgctg ctcacacttg ctcccgacac gctcaaccca gatttaataa
    11341 aaattcaaca aaccatactg gcataaacgc atctgtggta aaagcgacca ccacgcaaca
    11401 tgagatttgt tcaacattaa ctcatcggat cagttcagca actattgcat tagctaacaa
    11461 taaaaatgaa aataatttcc atgatacaga aaaaggaggc tgtcatggtc gcacccattc
    11521 ccgcgaaacg cggcagaaaa cccgccgttg ccaccgcacc agcgactgga caggttcagt
    11581 ctttaacgcg tggcctgaaa ttactggagt ggattgccga atccaatggc agcgtggcac
    11641 tcacagagct ggcgcaacaa gccgggttac ccaattccac gacccaccgc ctgctaacca
    11701 cgatgcaaca gcagggtttc gtgcgtcagg tcggcgaact gggacactgg gcaatcggcg
    11761 cacatgcctt tatggtcggc agcagctttc tccagagccg taatttgtta gcaattgttc
    11821 accctatcct gcgcaatcta atggaagagt ctggcgaaac ggtcaatatg gcggtgcttg
    11881 atcaaagcga tcacgaagcg attattatcg accaggtaca gtgtacccac ctgatgcgaa
    11941 tgtccgcgcc tatcggcggt aaattgccga tgcacgcttc cggtgcgggt aaagcctttt
    12001 tagcccaact gagcgaagaa caggtgacta agctgctgca ccgcaaaggg ttacatgcct
    12061 atacccacgc aactctggtg tctcctgtgc atttaaaaga agatctcgcc caaacgcgca
    12121 aacggggtta ttcatttgac gatgaggaac atgcgctggg gctacgttgc cttgcagcgt
    12181 gtattttcga tgagcaccgc gaaccgtttg ccgcaatttc tatttccgga ccgatttcac
    12241 gtattaccga tgatcgcgtg accgagtttg gcgcgatggt gattaaagcg gcgaaggaag
    12301 tgacgctggc gtacggtgga atgcgctgac tttttctggc gggcagaggc aatattctgc
    12361 ccatcatacc tgagtggcaa tagaataagg gtgtctgtta atcgcattga cgccaaaata
    12421 acttaatgtc atacacttca cacactttta ttaactcagc attattttta aacatcaaac
    12481 cacttaatta ttaacaaaat cgcaagatct cattccaccg aaacttttac ggctgcatta
    12541 tgagattgag cgatttatga tattttttta gttgtaatca tattaaattt tttcactgtt
    12601 ctgatagtta aaattcaaga catcaataaa caatgagata tttaaatgat tactcgtatt
    12661 cctcgtagtt ctttctctgc aaatattaat aatacagccc agacaaatga acaccaaacc
    12721 ctgagtgaat tgttttataa agaactcgag gataaatttt ctggcaagga gctggcgacg
    12781 cctctattaa aaagcttctc agagaactgt agacaaaatg ttcgtcatat ctttagcaac
    12841 aaggattttg tcattaaatt ttccacgtcc gtcttacaag ctgataagaa agaaattacg
    12901 ataattaata aaaacgaaaa cacgacactc actcaaacca ttgctacaat atttgaagaa
    12961 tacctaatgg aaattttacc tcaacgctca gacactcttg ataaacaaga attaaaccta
    13021 aaatcagata gaaaagaaaa agaattccca agaattaaac ttaatggtca atgttatttt
    13081 ccggggcgcc ccaaaaccgt atagtatacc gacacattgc tgcacaatat attaatgata
    13141 tttatcagaa tgttgattac aaaccccatc aagatgatta ctcttcagct gaaaaatttc
    13201 tcactcactt caacaaaaaa tgcaaaaacc agactttggc gttgatttcc agccgtcctg
    13261 aggggcgttg cgttgctgcc tgcggtgatt tcgggctagt tatgaaagca tattttgaca
    13321 agatggaatc aaatggcatc agtgttatgg cagccatatt actggtggat aaccatgctt
    13381 tgacggtccg gctaagaata aagaacacaa ctgaaggatg tacccattac gtgatttcgg
    13441 tttatgatcc taatgtaact aacgataaaa taagaattat gagcgaaagc aaagaggata
    13501 ttaaacacta ttctctgatg gattttatga atgtagatta tagcctcctg aaatggtcaa
    13561 atgatcatgt tattaaccaa tctgttgcaa taattccagc acttccgaaa gaacagctat
    13621 tgatgttaaa aggaactgtg gatgaaataa cccctccatt atcaccagca acgatgaatt
    13681 tgctaatggc aattggtcag aatcaccaac ttacgcaact gatgattcag ctccagaaaa
    13741 tgccagaact acatagaaca gaaatgttga ctgcctataa tagtattaac ctccccggtt
    13801 tatatttggc tataaattat ggcaatgcgg atatcgttga gactattttc aattcattgt
    13861 cagaaacagg atatgaaggg ttactctcga aaaaaaatct catgcatatt ctggaggcaa
    13921 aagataaaaa tggtttttct ggattatttt tagcgatatc acgtaaggat aaaaatgttg
    13981 taacctcgat tctgaacgtc ttacctaaac tggccgcaac acatcattta gataatgaac
    14041 aggtgtataa attcctgagt gccaaaaata gaacgtccag ccatgtttta taccatgtta
    14101 tggcgaatgg tgatgccgac atgctgaaaa ttgttttggt cgcgttacct ttgttaattc
    14161 gcacatgtca tttgactaaa gaacaggtac tcgatctcct gaaggcaaag gatttttatg
    14221 gttgcccaag actatacctg gcgatgcaaa atggacatag ctatatcgtg aaagttattc
    14281 tcgaagcatt gccctgtctg gcccaggaaa ttaacatttc agcttccgat attgtagatc
    14341 ttctgaccgc taaaagtctt gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac
    14401 atatgaacgt tattaatact atttttaacg cattacccac tctttttaat acgtttaaat
    14461 tcgataaaaa aatatgaagc ccctcctcct ggcaaataat tctaatgaat atcccggttt
    14521 gttttcagcg atacagcata aacaacaaaa cgttgtagag acggtttatc ttgctttatc
    14581 tgaccatgca cgcctgtttg gatttaccgc tgaagatatt atggattttt ggcaacacaa
    14641 agcgccacaa aaatactctg cctttgagtt ggcttttgaa tttggtcacc gggttattgc
    14701 tgaattaatc cttaatacat taaataagat ggctgaaagc tttggcttta cggataaccc
    14761 tcgatacatt gcggagaaaa attatatgga agctttactc aaaaaagcat ctccccatac
    14821 cgtacgctaa atctttgcct gcgccgatac gcataaacat cttccacatg cccttcacgt
    14881 atgcggtttt gtagtgcgcg ccagtaatca gcgcggaaca ggtcggcgtg catctcttca
    14941 aacagcggac caatacgcgg gtcggcgcat agccagtggc gaaactcttc cgggaaaaca
    15001 tcgcccggcg agacgctgta ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc
    15061 ggcgggatgt cgcggaaatt cacttccgtc atgtagcaaa tttcatcgta atcataaaaa
    15121 accacacgcc cgtgacgggt gacaccgaag tttttaaaca gcatgtcgcc agggaaaatg
    15181 ttagcagcgg caagctggcg aatagcgtta ccgtattctt caatggcgtc gcgcaactgc
    15241 tgaccttcca cttgttccag ccagatattg agcggcacca tccgccgctc gatatagaga
    15301 tggcgaatta caatctgctc accaagatcg gtgatttttt ccgctgcttc ctggagcagt
    15361 aattccatta ataccgggga aatatgccgc ttctccagca caaagttttc aaactcctgg
    15421 gtgtccgcca ttcggcccac acggtcgtgc tctttcacca gttgatagca ggcacgaacg
    15481 tgagcggcag acatctcttt ctgcggcgcg aacttgtctt tgatgacttt gaatacccga
    15541 tcaaagcccg gcagcgtaaa caccaacatc accattccac gaatacccgg cgcttcaatg
    15601 aactgctcat tacagccctg tagataaacg agatattcgc ggtagctttc ggttttggcg
    15661 tgcttctggc agccgatagc catatacaat tcagcagtgg ttttacctgg cagaatttcc
    15721 cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa ccataaaata agaacgcgca
    15781 aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc aggtatcaat aaataactcg
    15841 ccgtcgtccg tctggtggat cggcagtaag aagggcaacg tgccggaagg cgtgatcagt
    15901 ttgcccacca gccaggcggc tttattgcgg taaaacagtt cgttcgccac ctgtaaatga
    15961 ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat ggcgaatgat gtaatgaatg
    16021 tcacggcttt tattctgcca gcgcaggcgc agcggcaggt cgctgataac gcgcatcagt
    16081 agagattccc agccgtgatc ggggtgaaag tctttcgcca gcgggcgggg aatggtacga
    16141 aagcggcgct ctggctgaga gctaaagata aaaagccgct cgggagtaag cgagcggtgg
    16201 tcaaataacc gacagtacac ggagttaaaa aagctctccg caatctcgaa gcgcgggtaa
    16261 tccggcaaca gccgcgtgta atgctcttta acgcgcagta aaaatgccgc gtccgtactt
    16321 tggccgttag taatgcagcg cagttgctcc acgaccagac caacgtgatg atcgtaaaga
    16381 tggatacggt ttttcatcgc ctgctggaca gcatgccagt cggcctgttc gaaacgctgc
    16441 tgcgccccgg aagtcacttc gaggaatcga ccatactgag cgtcgaagcc ttgcaaaatg
    16501 gtttgagcaa tcagtaattc caggccacgc ggcatttagc gcactcatca ggagcagaga
    16561 attgccggat gcggcgtaaa caccttatcc agcctacgtt cggcaacggt tgtaggcatg
    16621 ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat tgccggatgc ggcgtaaatg
    16681 ccttatccgg cctacagtca gcaacgtttg taggttgctt agaactgcga ttcttcagtg
    16741 gagccagtca gcgcggtgac tgaagacgtg ccgccctgaa taatagtcgt cactttatcg
    16801 aagtaacctg tacccacttc ctgctggtga gatacgaagg tatagccatc tttcgcggcg
    16861 gcaaattccg gctgctgcac tttctcaaca tagtgcttca taccttcgcc ctgcgcataa
    16921 gcgtttgcca ggtcgaacat gttaaaccac atgctgtgga tacctgccag ggtgatgaac
    16981 tggaacttgt agcccatatc cgacaactgc tgctggaagc tggcaatagt tttgtcgtcg
    17041 aggtttttct gccagttgaa cgacggcgag cagttataag ccagcagttt gcccggatat
    17101 ttcgcgtgga tagcttgtgc aaagcgacgc gccagttcca gatccggcgt ggaggtttca
    17161 caccagacca ggtcagcata tggcgcatac gccaggccac ggctgatcgc ttgctcaatg
    17221 cccgcatgag tacggaagaa gccttcactg gtacgctcgc cggtaataaa ttcgctgtca
    17281 tacgggtcgc aatctgaggt gatcagatcc gccgcatcag catcggtacg tgcaaccagc
    17341 agggttggaa cgcccgtcac gtcagctgcc agacgcgccg cgaccagttt ctgaatagct
    17401 tcctgagttg gcactaaaac tttgccgccc atgtgaccgc atttcttcac tgacgccagc
    17461 tgatcttcga agtgaactgc cgcagcaccg gcttcaatca tcgctttcat cagttcaaag
    17521 gcattcagga caccgccaaa accggcttcc gcatcggcaa cgatcggcag gaagtaatcg
    17581 acatagcgcg gatcgcccgg ctcaatgccc acggaccatt ggatctgatc ggcacgacgg
    17641 aaggtgttgt tgatccgctc caccacagct ggcaccgagt ttgccggata gagcgactga
    17701 tccggataca tgctggccgc caggttagcg tccgccgcta cctgccatcc cgacagatag
    17761 actgcttcaa tacccgcttt cgcctgttgc agcgcctgac cgccagtcag tgcaccgagg
    17821 ctgttgatgt agcctttttt cgactcaccg tgcagcagac gccacatttt cgctgcgccc
    17881 agttgcgcca gcgtgcattc aggattgact gaaccgcgta atttcaccac atcttccgca
    17941 ctgtatgggc gagtaatgcc ttcccaacgc ggttgagtcc actctttctg taattcttca
    18001 atttgttgtg tacgggtttt catgtgcaga tgctccatag ttatgtggtg gtttacgcta
    18061 acaggcggta gcctggcagg gtcaggaaat caattaactc atcggaagtg gtgatctgtt
    18121 ccatcaagcg tgcggcatcg tcaaaacggc cctgggagaa acgttcttcg cccagttcgc
    18181 tggcaatgac tttcatctct tcgcccagca tctggcggaa caaggctttg gtcaccggtt
    18241 tgccattgct caacgttttt tgatgatgga tccactgcca gatcgaggta cgggaaattt
    18301 cagccgtcgc cgcatcttcc atcaggccat aaatcggcac gcagccgttg ccggagatcc
    18361 acgcttcgat gtactgcaca gccacgcgaa tgttggcgcg cataccttct tcggtgcgtt
    18421 caccatcaca aggtgccagc agctgatcgg cagtaatcgg cgcgtcttgt tcgcgcaata
    18481 cttcaagctg atttttacgg gagccgagaa tatcgttgaa taccgccatt gccgtgtccg
    18541 caaggcctgg gtgagcgatc catgtgccat cgtgaccgtt attggcttcc agcgctttat
    18601 ccgcttttac tttgttgagc acctggttat tacgctcttc atctttgctc ggaataaacg
    18661 ctgccatgcc gcccatcgca aaagcaccgc gtttatggca ggttttaatc agcaggcgtg
    18721 agtaagcatt caggaatggt ttatccatcg tcacggcctg tctgtctggc aggacacgat
    18781 cgggatagtt tttcaacgtt ttgatatagc tgaagatgta atcccagcga ccgcagttca
    18841 gaccaacaat atggtcgcgc agcgcgtgaa ggatttcgtc catctggaac accgcgggca
    18901 gcgtttcaat cagcaacgtc gccttgatgg tgccgcgcgg cagattaaag cgatcttctg
    18961 catagctgaa gacttcgctc caccaggccg cttcctgcca ggactgggtt ttcggcagat
    19021 agaaataggg accgcagccc tttgccaaca gtgcctgata gttgtggaag aaatagagcg
    19081 caaaatcaaa caggctaccg gggattgcct caccacgcca ggtgacatgt ttttccggca
    19141 agtgcagacc gcgtacccga caaatcaaaa ccgctggatt gggcttgagc tgataaattt
    19201 tgcctgcttc attggtatag ctgatggtac cgttaaccgc atcgcgcagg ttaatttgcc
    19261 cgtcgatcac tttgttccag tctggtgcca gtgaatcttc gaaatcggcc ataaagactt
    19321 tcacattggc gttcagtgcg ttgatcacca tcttgcgctc taccgggcca gttatctcta
    19381 cgcggcggtc ttctaagtcc gcaggaatcc cgcgaatttt ccaatcagca tcgcgaatgg
    19441 aagccgtttc cgaaataaaa tcaggcaacg ttccgttatc aatatcttgc tgctgctgaa
    19501 tgcgcgctgc cagaagttta ttgcgttgtg gcgtaaaatg cgtcaccagc tcagtcagaa
    19561 attctaccgc ttcggcagta agaatttgct tctcctgctc gccatacggc cttgtgaaag
    19621 ccagttcatc ggttgttgtt gcctgttcag tcatcgtgca gctcctcgtc atggatccga
    19681 aaacttcccc tctgtgaacg aaggaatgtt gtgcgctttt cgttcagcac aactaagact
    19741 actcatttaa aatgcaaaaa caaaaacaat ttccatttta attaaaaata cttgataact
    19801 tattgataac aatatgaata aaatttattc acttgctgag gtgcgtttcg gaaataaaaa
    19861 aggcacccga aggtgcctga ggtaaggtgc tgaatcgctt aacgatcgac tatcacagaa
    19921 gattaatcca gcgttggatt catgtgccgt agatcgtatg gcgtgatctg atagacgtaa
    19981 tagttgagcc agttggtaaa cagcaaatta ccgtgactac gccagctcgc tcgcggtttg
    20041 ttttgcggat cgttgtgcgg gaaatagtta tacggtacat ccgggtctag tccggcttcc
    20101 acatcgcgga aatattcctg cgccagcgtt tgcgcatcat attcgggatg gcccgtcaca
    20161 aaggcaatgc gcttatcttt gctggcaaac agatatgcat ccccttcttc cgtctctgcc
    20221 agaatttcca gatcggtgta atcacgaatc aacgctgccg gaaagtcagc atagcgcgaa
    20281 tgcggtgcca ggaatgaatc atcaaagcca cgcgtcagaa gcgcatgagg atggagaata
    20341 tgatgctcgt aaacgccaga gagtttgtcg gtgcgagttt gcttaggaat gccgtagagg
    20401 atattgagcg cggcctgtac cgcccagcag acaaacagcg tcgaggtgac gtgatctttc
    20461 gaccactcca gcacctgttt gatctgcggc cagtaagcga catcattaaa ctccaccagg
    20521 cccagcggcg caccggttac aatcagaccg tcaaagttct gttcctgaat atcttcaaag
    20581 ttacagtaga agttgttcag atgctctgcg ggcgtgttgc gcgattcacg ggaatcgatg
    20641 cgcaacagct gaatatcgac ctgcaaaggt gagtttgaaa gcaggcgcag aaactgattt
    20701 tcagtttcaa tcttcttcgg catcaggtta aggatcagaa ccttaagtgg acgaatttcc
    20761 tgaccagacg cacgagaagt tgtcatcaca aagacgtttt cttcacgcaa gaaattgacg
    20821 gcgggtagct cgtccggcac acgaatcggc ataacctgat tacctcacta catacgctta
    20881 tacgtttaga catccagata gctgaagata accagaaaat ttgccaatgt cgagcctgca
    20941 tgttgaaggt gagaaagttt caaggaaaag gctacgttag aatataagaa tgacgaaaag
    21001 gagagaaaat ggttattagt attcgccgct cacggcatga ggaaggggag gaactcgttg
    21061 cgatttggtg tcgatctgtc gatgccaccc acgatttttt atcagcagag tatcgggccg
    21121 agctggaaga gctggtgcgt tcctttctgc cggaagcacc gttgtgggtc gcggttaatg
    21181 aacgggagca ggcggttggg ttctataaga aggtgggttt taaggttacg ggacgctctg
    21241 aggtggacga tttggggaaa ccgtatccgt tgctgaatct ggcgtatgtg ggggaataag
    21301 ccgcccctgc cgtgcctgcc caagctggag aagacgcgcc agcgtcgcac ccggcatttt
    21361 tttcac
//
LOCUS       DAJTYG010000063        21359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTYG010000063 DAJTYG010000000
VERSION     DAJTYG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.063"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..76
                     /locus_tag="OO568_002549"
     CDS             <1..76
                     /locus_tag="OO568_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6641863.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            92..1018
                     /locus_tag="OO568_002550"
                     /pseudo
     CDS             92..1018
                     /locus_tag="OO568_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin"
     gene            1257..1316
                     /locus_tag="OO568_002551"
     CDS             1257..1316
                     /locus_tag="OO568_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6641864.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1633..1692
                     /gene="ibsE"
                     /locus_tag="OO568_002552"
     CDS             1633..1692
                     /gene="ibsE"
                     /locus_tag="OO568_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR6641865.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1812..3245)
                     /gene="hldE"
                     /locus_tag="OO568_002553"
     CDS             complement(1812..3245)
                     /gene="hldE"
                     /locus_tag="OO568_002553"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6641866.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3293..6133)
                     /gene="glnE"
                     /locus_tag="OO568_002554"
     CDS             complement(3293..6133)
                     /gene="glnE"
                     /locus_tag="OO568_002554"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6641867.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAAAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6156..7457)
                     /gene="ygiF"
                     /locus_tag="OO568_002555"
     CDS             complement(6156..7457)
                     /gene="ygiF"
                     /locus_tag="OO568_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6641868.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7699..8319
                     /locus_tag="OO568_002556"
     CDS             7699..8319
                     /locus_tag="OO568_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6641869.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPNRKRKDRWMN"
     gene            8383..9621
                     /gene="cca"
                     /locus_tag="OO568_002557"
     CDS             8383..9621
                     /gene="cca"
                     /locus_tag="OO568_002557"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6641870.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9784..10605)
                     /gene="bacA"
                     /locus_tag="OO568_002558"
     CDS             complement(9784..10605)
                     /gene="bacA"
                     /locus_tag="OO568_002558"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6641871.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10696..11064)
                     /gene="folB"
                     /locus_tag="OO568_002559"
     CDS             complement(10696..11064)
                     /gene="folB"
                     /locus_tag="OO568_002559"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6641872.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11169..11786
                     /gene="plsY"
                     /locus_tag="OO568_002560"
     CDS             11169..11786
                     /gene="plsY"
                     /locus_tag="OO568_002560"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6641873.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11799..12730)
                     /gene="ttdR"
                     /locus_tag="OO568_002561"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11799..12730)
                     /gene="ttdR"
                     /locus_tag="OO568_002561"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            12937..13780
                     /gene="ttdA"
                     /locus_tag="OO568_002562"
                     /pseudo
     CDS             12937..13780
                     /gene="ttdA"
                     /locus_tag="OO568_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13777..14382
                     /gene="ttdB"
                     /locus_tag="OO568_002563"
     CDS             13777..14382
                     /gene="ttdB"
                     /locus_tag="OO568_002563"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6641874.1"
                     /translation="MKKILTTSIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14430..15893
                     /gene="ttdT"
                     /locus_tag="OO568_002564"
     CDS             14430..15893
                     /gene="ttdT"
                     /locus_tag="OO568_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6641875.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLLLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYPPTMVMVTLIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(15936..16949)
                     /gene="tsaD"
                     /locus_tag="OO568_002565"
     CDS             complement(15936..16949)
                     /gene="tsaD"
                     /locus_tag="OO568_002565"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6641876.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNSTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17187..17402
                     /gene="rpsU"
                     /locus_tag="OO568_002566"
     CDS             17187..17402
                     /gene="rpsU"
                     /locus_tag="OO568_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6641877.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17513..19258
                     /gene="dnaG"
                     /locus_tag="OO568_002567"
     CDS             17513..19258
                     /gene="dnaG"
                     /locus_tag="OO568_002567"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6641878.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVIFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTSGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19453..21294
                     /gene="rpoD"
                     /locus_tag="OO568_002568"
     CDS             19453..21294
                     /gene="rpoD"
                     /locus_tag="OO568_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6641879.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5155 a         5896 c         5484 g         4824 t
ORIGIN      
        1 actgtcgttc tcaaaatcgg tggagttgca tgacaaggtc atcgggcatt atctgaacat
       61 aaaacactat cagtaagttg gagtcattac cgaacaacaa gaagcgttta tgacgcttga
      121 gcaggagcag caggttaaaa cccgtactgc cgaacagaat gcacgtattg cggcttttga
      181 agctgaacgt cgtcgtgaag cagagcagac gcgaattctg gctgaacgac aaattcagga
      241 aacggagatc gagcgcgagc aggccgtccg ctcaagaaag gttgaggctg aacgtgaagt
      301 tcgtattaaa gagatcgaac agcagcaggt caccgaaatc gccaaccaga cgaaatcgat
      361 cgctattgcc gccaaatcgg aacaacagtc ccaggcagaa gcgcgtgcta accttgcact
      421 cgcagaagca gtaagtgcgc aacaaaacgt agaaaccact agccagaccg cagaagccga
      481 tcgtgctaaa caagttgccc taatcgctgc cgcgcaggat gcagaaacca aagcggttga
      541 actgaccgtg cgggcgaaag cagaaaaaga agccgcagag atgcaggcgg cggctatcgt
      601 tgagttagcc gaagctacac gtaaaaaggg tctggcggaa gcagaagcac aacgtgcgct
      661 gaacgatgct atcaacgtac tttctgatga acaaaccagc cttaaattca aactggcctt
      721 gttgcaggcg ctacctgcgg taatagaaaa atccgttgag ccgatgaaat ctattgacgg
      781 tatcaagatt attcaggtcg atggtctgaa tcgtggcagc gctgcgggtg atgcaaacac
      841 gggtaatgtg gggggcggaa acctagcgga acaagcatta tcagccgctc tctcttaccg
      901 cacacaggct ccgctgattg actccttgct caatgaaatt ggcgtttcag gcggttcact
      961 ggcgacattg acttcaccct taacctcaac aactcccgtc gccgaaaacg tagaataaat
     1021 tttcagctat gaacgtccct ggcacaaaaa gccggggatt ttttatatct gcgttccgct
     1081 aaaaactgca aatgatcagg tcgttgcaac gttttgcgtg accgctcggg gaaggcaaaa
     1141 ctgcctctgg gaaagcgttg cgcgaggtcc ggcgctcatc aacaatcggg gagtcagcaa
     1201 agggctgaaa cgggaaagcc cctcccgagg aaggggcctt acagaaggaa agggttatga
     1261 tgaagctcgt catcatactg attgtgttgt tactcgtaag tttcgcagct tattaacagc
     1321 caatcagagg ggggagaaat cctccctcac ccttgttcct ttactttagg ttgaaaaaaa
     1381 atcagcgtca agaaggtttc attaacacat atccctggcc caaaaagccg gggatttttt
     1441 atatctgcgt tccgctaaaa ggtgcaaatg ctcaggacgt tgcagcgttt tgcgtgaccg
     1501 ctcggggaag gcaaaactgc ttcagggaaa gcgttgcgcg gggtccggcg ctcatcaaca
     1561 atcggggagt cagcaagggg ctgaaacggg aaagcccctc ccgaggaagg ggccttatag
     1621 aaggaaaggg ttatgatgaa gttcgtcatc atactggttg tgttgttact gttaagtttc
     1681 ccgacttact aacaactgat cagagggggg agaaatcctc ccttaccctt gttcctttac
     1741 tttaggttga aaaaacaaca gcgtcaatag gcctgcctgc tacgaagcga gatctgtgaa
     1801 ccgctttccg gttagccttt tttatcctgt tggatcttct tgatgatgtt agtggtcgag
     1861 caaccgtctt cgaagttgag caccagcact tcgccgccgt tggcccagac ttctttactc
     1921 ccggcgatct cttctggttt atagtcgccg cccttcacca gtaaatctgg caggatcccg
     1981 gcgatcaagc gctgcggcgt gtcttcttca aacgacacca cccagtcgac cgcttccagt
     2041 gcgcccagca caatcatacg ctgttcgagc gggttaacgg ggcgggaatc ccctttcagc
     2101 cgtttggtgg aggcatcgct gttcacagcg acaatcaagc ggtcacccag tttgcgggca
     2161 tttgccagat agaagacgtg tccggcgtgc aggatgtcaa agacgccatt ggtcatcacc
     2221 actttttcac cgcgtttacg cgctgccgct acggccagct tcagttcctc ttcggtcatc
     2281 acgccaaagc cggtatctgc gcgcccacgc actgcatttt ccagctcgat cggcgaaacc
     2341 gtggaggttc ccagtttgcc gaccaccacg ccagccgccg cattggcaaa gaagcaggct
     2401 tcttccagcg aattacccgc tgccagcgtt gccgccagga cgccaatcac cgtgtcgccc
     2461 gcaccggtaa cgtcatacac ttcctgcgcc tgggttggca tatgcagcgg cgctttaccc
     2521 ggttgcagca gcgacatacc ctgttcggaa cgggtcacta acagagccga gagttcgtaa
     2581 tcggcaatca gtttcatgcc gcgctcaaca atctcttctt cggtcttaca tttaccgaca
     2641 acagcttcaa attccgagag attcggtgtc agcagcgtag cgccgcggta gcgctcaaaa
     2701 tcggtacctt ttggatcaat cagcaccgga acgcccgctt tacgcgccag ttggatcatc
     2761 tgctgtacgc ttgccagcgc acctttggcg tagtcagaaa gcaccagcgc gccaatcgaa
     2821 ctcagcgcct gattaatccg ctcgtgcagc ggctgcggat caacaccttc gaaaccttct
     2881 tcaaaatcca gacggatcag ctgttggttg cgggaaagta cccgtaattt ggtgatggtc
     2941 ggatgcgtcg gtacagaaac gaagtcgcat ttgacgttga cgtcggccag agatttactc
     3001 agcgcgcgcg ctgcatcgtc aatgcccgtc aacccgacca ggcgtgcatt agcaccgaga
     3061 gaagcgatat tcatcgccac gttagccgcg ccgcccggac gttcttcgat ggtattcact
     3121 ttaaccacgg gcaccggcgc ttccggcgag atacgactgg tggggccgta ccagtaacga
     3181 tccagcatca catcaccaac caccatcact cctgcacgtt caaactctgg cagcgttact
     3241 ttcattcctg tctcctgaga gattcaaaat ttgcgcgcga taataccata cttcattctt
     3301 ccaccagcca cttctgccag cttgcccgca ccagatcacg ctctgcggtg aagcaatcct
     3361 ccgacacatg gcccggcaat tcctgtaatg ccagatgatg aagttcatcg cgcagcgtcg
     3421 tgtaagcacg ggtcagcgcc atcgcttcct gctcttccat aatgtcgttt tgcgccagca
     3481 gctccagaat acgcacgttg tctgaccagc gcgttaactt cggtttttca tgagcgtagc
     3541 gcaacaccag atattgggta ataaattcga tatcggtaat tccgccttca tcggctttga
     3601 tatcaaagcg attacgatgt ttattgccga gatgagcgcg cattttctcg cgcatttccc
     3661 gcacttccgt ttgcagagtt ttaccttctc gcggcagtgt cataatctca cgacgcaccg
     3721 cgtcaaagtg cgcagtgagc tgcggatcgc cgtacactac acgcgcacgc accagcgcct
     3781 gatgttccca cgtccaggcc tcgtttttct gataatcggc aaatgctgct gcggatgtca
     3841 ccagcattcc cgccgccccg gacggacgca gtcgagcatc cacttcatac aaaatgccgg
     3901 aagaggtacg cgtactgaac agatgcataa tgcgttgcgc cagacgcaga taaaactgcc
     3961 gcccgtcgat ttcccgctcg ccgtcagtca tcgcatccat tgggcaatca tggaggaaga
     4021 taaggtcaag atcggaactg tagcctaact cccagccgcc cagcttgccg tagccgacca
     4081 ccgcaaaacc gcgcccttca cgttcgttga ggtgatttgg cttaccgtag cgggccacca
     4141 tttgacccca cgcctgctga acgacggcat ctatcatagc ttctgccagc caggttaagt
     4201 gatcgctcac tttcattacc ggtagcgtac cggcgatatc cgctgccgca atgcgtaaca
     4261 gctgcgcctg tttgaactga cgcagcgcct caagctgttg ttcttcgtca tcttccggca
     4321 cgcgcagcaa atactggcgc agttcatcac ggtaggcatc ggtcgccgtc ggctggtaaa
     4381 gggtatttgg atcgagcaat tcatccagca ataatggata acgcgctagc tggctggcaa
     4441 tcatcggcga tgcggcacac agagaaatca aatgtttaag cgccgcaggg aattcactga
     4501 gcaattctaa ataggttgtg cgggtaacaa tccccaccag caaggcggta atgcgcgaca
     4561 gcgtgacagc agcgtcttca cgcgcacaga catcacttag cagatgtggc atcagatggt
     4621 cgagcacctg acgtcctcgc ggcccgatag tacgcttatc cagctctttg cggaaatcag
     4681 caatcagcgt taggacctgt ttgcgatcat cctcgctaag atgcgccagc actggcgtag
     4741 tgtcatcttc ctgcaacgca tcctgccaca gctcacgcca ctgttccgac agcgactctt
     4801 cctgagtttc actttcgtcg tcgccaatca attcattaaa cacccggcgc acattggtca
     4861 tatgtgcggt cagcgccccg gtcagttgcg gccagtcagc aaagtccatc gcccacgcca
     4921 gccgcgcacg gttaagctca tcagaaggaa gcgtttgggt ttgttcgtcg ttaatgcttt
     4981 gcagcaggtt ttccagacgc cgcaggaaca gatatgccac tcgcaattgt tcagcatcgt
     5041 tttcagaaag cagatgcagc gcggcaatgg cgctgagcgt tggcagtaaa gagcgcgact
     5101 gcagcaacgg ttcgcgccca ccgcgaatga gctggaacac ctgaacgata aattcaattt
     5161 cgcgaatgcc gcctgcgccg agtttgatat tgtcggtcaa accacgtcga cgcacttcac
     5221 gggcaatcat ccctttcatg ttgcgcagcg actgaatcac gctgaaatcg atgtaacgac
     5281 ggaaaacaaa cgggcgcagc atcgcacgca actcgttagc atagacgcct tcgctatcgc
     5341 ccataatccg cgctttgacc atcgcgtaac gctcccagtc gcgtccctgc tcctggtaat
     5401 aatcctccaa cgcggcaaag ctcagcacca gcgggccact ttcgccaaac ggacgcagtc
     5461 gcatatccac gcgatagacg aagccatcct gcgttggttg atccagcact ttaatcagcc
     5521 gctgccccat gcgggtaaaa aactgcgcgt tatccagttc ccggcgtccg ccctgcgtac
     5581 aaccatgttc cggccaggca aaaatcagat cgatatcaga ggagaaattt agttccccgc
     5641 cgcccagctt acccatgcct aagatcagca gcggttgcgc ttcgccctgc gcattgcacg
     5701 gcgttcccca ctcgcggcag caggcgtcat acagccagtc acgcgccgca acaatcagcg
     5761 tctccgccag ataactgagc tgctgcaata tgctctcttc agtaaccagt gccagcgttt
     5821 gcgcccatgc gatgcgcacc ataatgcgcc gccggaatag ccgcagctcg cgcattaacc
     5881 cggcttcgtc gctcacatta ctgagcgcct cctgcaacca cacgctgtaa tgttgccatt
     5941 cgtcagcctg tggcggttgg ctttccagtt ccgtcagcca ctctggatgc gcaatcacgc
     6001 tgtcctgcac aaaatcactg aaagtaagta ctgacttcgc ctgtgcacta agtgattcct
     6061 cggctaaaag ctctggcagc cgctcaacaa cggtctgcca gtactgctgt aacggtgaag
     6121 agagcggctt cataagcgat tttatccttg cctgattaac gttttccgct gtgcaaccag
     6181 aacggttcct gattgtttgc ctcattacgg aaatgttcaa tttcaatgcg ttgcccggtc
     6241 gcaatagcgt gacgcagccc ctgccagttc tccagccagg cttgcgcgac gacaggatca
     6301 tagtaacccg ccagcaacag tattgagtca atatcacgca tcaggcgtgg caactggtca
     6361 cggtagcgat cgcctaacgg ctggcagaaa acgcttttca gttcagcggc atggcgggaa
     6421 agatggatat cggcaaagcg tttgaaggag tcgctaattt tgccctgcgc tttggcatct
     6481 aaaaacggct gccatgcttt gcttaccagc cattcggtca acgccagctt cgccattgcc
     6541 gtttcggtag agtagaccgc cgtcacggca gaaaccgcag aagcgatggt cgcttcgcat
     6601 tgagtcagca aatcacgtaa gtgagtgctc gctttacgcg gcacaatgcc accgaacagc
     6661 atcagtgtat gacggactag gctaatggct gccagcacct gctctttcgc cgcatcgttg
     6721 ccgcgtaccc acagttcttc atgatactgc cattgcgcta acaccagctc gagcgccgct
     6781 tccagcccct gttccacatc ggcttttgcc gcaacatgca aaatggtggt cggtttgatt
     6841 tcacgcgccg gattgccctg cgccagatga tagccacgcg ccgctttgct caggctgccc
     6901 tggcgtaatc cggtttgcga taccagttgg ttcgccagtt tcagcaccgc gcgcgtgtcg
     6961 ccgctaagca gctccagttc cagctcgcag ataggctcag caaattcacc cgctttcact
     7021 tccccctggt cgagagcgat ttcaattcgg ctgccatcga tctctactag ccatttttcg
     7081 cgataaaaat cggtgctaaa cagcggctgc acgcgggaag cgagatcggc aggcaattcg
     7141 ccgttcggcc agatttccgt cggtaactgc gccaggtcga gcgtcggttc gctcaacgcc
     7201 acgttatatt ccgggcgctg gtgtaagccg cctgtcactc ttcctgcaac tttcatggtc
     7261 atctcatagc gaccgttttc gccacgaata cgtaagccca tatcgtgcct acgcagccag
     7321 ttatccggcg tttcgtagta aatattcagc aactgcacgg ggtcatggtg ctcgccgccc
     7381 agcgtattga gatggtcacg caacgcctca acggcactgt gattaacaat aaactttaat
     7441 tcgatttcct gagccatggc ctttacttat cggttatgtc acataagtga cgatgaacgg
     7501 cgaacttaat gcgatctttt ttgtcagtag atagtatttt gcgccaaatt gccatgcaac
     7561 gggcaatttg acgggcgtaa aagtttgaag cagtggcaag acctgacaca gaggattccg
     7621 attgatgtcg aatgctttgc gtagcgacac tgatgccact actatcgttc cactttccat
     7681 gacaataacg acagcctgat gccaaaatta cgcctgatcg gattaacttt actcgcactt
     7741 agcgcgactg ccgtctcaca cgctgaagaa acgcgctatg tttctgacga actgaatacc
     7801 tgggtccgta gcggtccggg agatcattat cgcctcgtgg gcacggttaa cgccggcgag
     7861 gaagtgacct tattacaaac tgacgccaac accaattatg cccaggtgaa agacagctct
     7921 ggccgtaccg cctggatccc gttgaaacaa cttagcactg agccaagcct gcgctcccgt
     7981 gtgccagatc tggaaaatca ggtcaaaacc ctgaccgata aactcaccaa tatcgataac
     8041 acctggaatc agcgcaccgc agaaatgcag caaaaagtgg cgcagagcga cagcgtgatc
     8101 aacgggttaa aagaagaaaa tcagaaactg aaaaacgagc tgattgtcgc gcagaaaaag
     8161 gtcgatgccg ccagcgtaca gctggatgac aaacagcgca ccatcatcat gcaatggttt
     8221 atgtatggcg gtggcgtgct ggggcttggc ttgctgctcg gtctggtact gccgcacctg
     8281 atcccaaacc gcaaacgcaa agatcgctgg atgaactaaa tcgccttctt cgttgcactg
     8341 tcttattatt aggaattgtg tatttgacga agggatgatg gcgtgaagat ttatctggtc
     8401 ggtggtgctg ttcgggatgc attgttaggg ctatcggtca aagacagaga ttgggtggtg
     8461 gtcggcagta cgccacagga gatgctcgac gcgggctacc agcaggtagg ccgcgatttt
     8521 cctgtgtttc tgcatccgca aacgcatgaa gagtatgcgc tggcacgtac cgaacggaaa
     8581 tccggttccg gttacaccgg ttttacttgc tatgccgcac cggatgtcac gctggaagat
     8641 gatcttaagc gtcgcgatct gaccattaat gcgctggccc aggacgataa cggcgagatt
     8701 atcgacccgt acaacggtct gggcgatctg caaaatcgtc tgttgcgcca tgtttccccc
     8761 gcttttggcg aagatccgtt acgcgtactg cgcgtggcgc gttttgctgc gcgttatgcc
     8821 cacctcggtt ttcgtattgc cgatgaaact ctgacgctga tgcgcgagat gacccatgcg
     8881 ggtgaactgg aacacctgac gcctgaacgg gtatggaaag agacggaaag cgccctcacc
     8941 acccgcaatc cccaggtgtt cttccaggta ctgcgcgatt gcggcgcact tcgcgtttta
     9001 ttcccggaaa ttgacgcact gtttggcgtt ccggcacctg ccaagtggca tccggaaatc
     9061 gacacgggta ttcatacctt aatgacgctc tcaatggcgg cgatgctgag tccgcaggtt
     9121 gatgtccgtt tcgcaacttt atgccacgat ctcggtaaag gactgacgcc gccagaactt
     9181 tggccgcgtc atcatgggca tggcccggcg ggtgttaagt tagtggaaca actatgccag
     9241 cgtctgcgcg tgccaaatga aattcgcgat ttagccagac tggtggctga gtttcacgat
     9301 ctcatccaca ccttcccaat gctgaaccca aaaaccatcg tcaaattatt tgattccatc
     9361 gatgcctggc gtaaaccgca gcgtgtcgag caactggcgc tgaccagcga ggctgacgtg
     9421 cgcggcagaa ccggttttga atcagcggac tacccgcaag gccgttggtt gcgcgaaggc
     9481 tgggaagtgg cgcagtcagt gccgacaaaa gcggtcgttg aagcgggatt taaaggcgtg
     9541 gagattcgcg aggagctgac ccgacggcgg attgcggcgg tagccagctg gaaggaacaa
     9601 cgttgcccaa agcctgactg agtataaaac taaacgttag cacgaaccgt cccctcgccc
     9661 cttcggggag agggttaggg tgaggggaac agaccagcac cggtgcgaac aatcaccccc
     9721 accccggccc tctccctaca gagcgaggga gaaaacccgg cctgctcccg aaccttttct
     9781 caatcaaaag aacacgacat acaccgcagc cgccacaata aagcgataaa tggcgaacgg
     9841 gataaacgaa atgcgcttaa tcaattgcag gaaggtttta atcgctatca gcgccaccac
     9901 aaaagcggtg ataaacccaa cggcaaacat cgggatatcg ccggttgtca ggaagcccca
     9961 gcttttgtag agatcgagcg ccgttgcgcc catcatcatc ggtaccgcca gcaggaacga
    10021 gaactcggaa gcagcgtaac ggctcacgcc catcagcatc ccacctgaaa tggtcgcccc
    10081 ggaacgggag aaacccggcc acagcgccag acactggaaa cagccaatca taaacgcctg
    10141 acgataggtc atatcatcaa gacccggcgc acgcggctct ttcggcttca ggcattcggc
    10201 ggcaatcagc aacaaaccgc caacgaccag cgcatacatc acatttatcg ggttaaacaa
    10261 tgacttaatt gtgtcgtgga ataatagccc cagtaccacc gccggaatca tccccagcaa
    10321 aatgtggatc agcgttaaac gacctttgct ttcaccttcg tgctgcaacg ggcggccaaa
    10381 gtggatgcca atcaggccaa acagacgccg ccagaacatc actactaccg ccagaattga
    10441 tcctaactgg atcacaactt caaaggtttt cgccgtgtcg ccctcaaatc ccaacaagtg
    10501 accgacaata atcatatggc ccgtgctgga taccggcaga aattctgtca atccttcgac
    10561 cacacccaat attgccgcta tcagcagcga gtgcatatcg ctcatcaata aacccctaaa
    10621 ttattaaaat gtaccgcttg tccgaactac tgcagtatga ccaggttata accgtttggt
    10681 ttaacagctg taaaattaat tattttcttt cagattattg ccacgctcaa tgattacgcc
    10741 aacattcgcc gcccgcgcca ctgcgcctgg cttgctgagt ttgatacgca cccacggcga
    10801 gttgaagcgt gctaacagca gctccgccac ctcttcagcc acgcgttcca ccagcgcaaa
    10861 acgcgccccc tcgacgtggc tgaccaccgt ttctgcaatg tcagcgtaac tgaggcaatc
    10921 cgccacatca tcacttttcg ccgctttacg gttatcccat gccatttcga tatcgaacac
    10981 taacttctgt tcgatggtct gttcccagtc gtaaacacca atagtggtga ttaccgaaag
    11041 ttgctctata aatacaatat ccatcacgtc ctgcctgctt tttggctaac cggataccac
    11101 ttccggcgaa atgtgcgtat tatccacaga ttcatcgttg aacacgaatt ttcaaaacgg
    11161 aacagcttat gagtgcaatc gcgcctggaa tgatcctcat cgcgtacctc tgcggctcca
    11221 tttccagtgc cattctggtt tgccgcttgt gtgggctgcc cgatccgcga accagcggct
    11281 ccggcaatcc aggcgcaacc aatgtgttac gtatcggtgg caagggagca gccgtagcag
    11341 tactgatttt cgacgttctg aaaggaatgt tgcctgtctg gggcgcgtat gaattaggtg
    11401 tcagcccctt ctggctaggc ttaattgcca tcgccgcctg tcttggacac atctggcccg
    11461 ttttcttcgg ttttaaagga ggaaaaggcg ttgctaccgc ttttggagca atcgcaccaa
    11521 ttggctggga tctcaccgga gtaatggcgg gaacctggtt actgaccgtg ctattgagcg
    11581 gatactcgtc gctgggagcg attgtcagtg cactgattgc tccgttttat gtctggtggt
    11641 ttaagccaca attcaccttc ccggtttcga tgctctcttg cctgatcctg ctgcgtcatc
    11701 atgacaacat ccagcgtctg tggcgtcgtc aggagacaaa aatctggacg aaattcaaaa
    11761 gaaagcgcga aaaggatccc gagtgatttc tggtggatct acatgacctg atagccttca
    11821 tcgggcttgc ccagccgttg ctggcaccat gccgccagaa attccacgca gacacgaatt
    11881 tcatgctgcg atagagcggc tcccggtaaa cagcccagat attggcgctt tgtgtatact
    11941 ccggcaatac ttgcaccagt ttgccactct ccagaaacgg caacacatcc cactcggaac
    12001 gcagcataat ccctttgccc tccagcgccc attgcaacac tatctcgccg ctattggagg
    12061 aaagatgccc gctaaccttc accgattttt tctcctgacc gttccccaac tcccatattc
    12121 cgtgggtcat atcgcgctct ttggtcacca gacagtcatg acgacttaat tcctgtaatg
    12181 attgtggctg cggatatttt tgcagatact caggtgctgc gcataatatt cttttatttt
    12241 tcgttaacag atgcgcaata taataatcag gaatttcgtc attaatacga atatccagat
    12301 caatattatc ctgcactaaa tcaatttgcc gatcgaacaa ttcaaaatgc acctgtaact
    12361 caggataatt gcgcataagt tcggtaatgg ctggcgcaat atggctgcgc ccaaaaccaa
    12421 aactgcaacc aatacgaatc atcccctccg ggcgcgtttt gatttgcgtg acgtcatcga
    12481 ccagtcgctg atattgggtg aggatttcca gcgcatgttc gtaacaacgt tggccacttt
    12541 cagtcagcgc cacgccgcga gcggagcggt tcagcagcgt tgttgccagc gtattttcaa
    12601 ggatttggat ccgcttggtg acaaatgcag gcgtttgccc aagcgtcgcc gctgcagcac
    12661 taaaactccc gctgtggacg atttctacca gtacctgcaa gtctttggct aaaggccagc
    12721 tattcagcat cgttaattat ccgcagttgt gataagcgca gtgtatttcg caaaacattg
    12781 ccattccctg ctctggcgca atattcattc ttattttcgc gttgattcac gaactgttaa
    12841 ttacatattc acggtggcaa aaaatataaa accacatttt tagtggtagt ttgtggcggt
    12901 gaatttttcc agacaaatac aaaaactgga gttgccatga tgagcgaaag taataagcaa
    12961 caggcagtga ataagttgac agagattgtc gctaacttta ccgccatgat ttctacccga
    13021 atgcctgatg acgtggtgga taaactaaaa cagctaaagg atgccgaaac gtcgtcgatg
    13081 gggaaaatta tctaccatac gatgttcgac aacatgcaaa aagcgattga cctgaatcgt
    13141 cctgcctgtc aggacaccgg ggagattatg ttcttcgtta aagtcggttc ccgcttccca
    13201 ctgcttggcg agctgcaaag catactcaaa caagccgtgg aagaggcaac cgtcaaagcg
    13261 ccactacgtc acaatgcggt agaaattttt gacgaagtaa acaccggcaa aaataccggt
    13321 agcggcgtac cgtgggtcac ctgggacatc atccccgaca atgacgatgc ggaaatcgaa
    13381 gtttacatgg caggcggcgg ctgcacgcta cctggccgct cgaaagtgtt aatgccgtca
    13441 gaaggctacg aaggcgtggt gaaattcgtc ttcgaaaata tctccaccct cgccgtaaac
    13501 gcctgtccac cggtactggt gggcgtgggc atcgccacct cggtggaaac cgccgccgta
    13561 ctctcgcgta aagccattct gcgcccgatt ggcagccgcc atcccaatcc aaaagcggca
    13621 gaactggagc tacgcctgga agaaggactc aaccgtctgg ggattggtcc acaagggctg
    13681 accggcaaca gttcagtgat gggcgcatcg tcgcggcacg ctgctggttc atgccgatct
    13741 cacctttgaa aatctgtctc acacccggag cgcgttatga aaaagatcct gacaacctcg
    13801 atcaaagctg aagatctgca agatattcgc gtcggcgatg tgatctacct gaccggtacg
    13861 ctggtgacct gccgcgacgt ttgtcaccgc cgtttgatcg aactgaaacg tccgatccct
    13921 tacgatctca acggcaaagc gattttccac gctggcccca tcgtgcgcaa aaacggcgac
    13981 aaatgggaga tggtctccgt cggcccgaca accagtatgc gtatggaaag ttttgaacgt
    14041 gaatttattg agcagaccgg cgtaaaactg gtggttggca aaggtggtat ggggccgctg
    14101 accgaagaag gctgccagaa attcaaggcg ttacatgtga ttttcccggc aggctgcgcg
    14161 gtgctggcgg caacccaggt agaagagatt gaagaagtgc actggacaga actcgggatg
    14221 ccggagtcac tgtgggtctg ccgggtcaaa gagttcggcc cgctgattgt ctctattgat
    14281 acccacggca acaacctgat agccgaaaac aaaaagctgt tcgccgaacg ccgcgatccc
    14341 atcgtggaag agatctgcga gcacgtccat tacatcaaat aaccctcccg gagaggctca
    14401 cccctctcct ttttcgcagg cataacacga tgaaaccttc cactgaatgg tggcgatatc
    14461 ttgcaccgct ggcggtcatc gccattattg ctctacttcc ggttcccgca gggctggaga
    14521 accatacctg gctctacttt gccgttttta ctggcgtgat cgttggactg atcctcgaac
    14581 ccgtgccggg tgccgtggtg gcgatggtgg gtatctccat catcgccata ctctctccct
    14641 ggctgctgtt cagccccgaa cagctcgccc agccaggctt taagttcacc gccaaatccc
    14701 tctcctgggc agtttctggt ttttctaatt cggttatctg gctcattttc gccgccttta
    14761 tgtttggcac aggctatgaa aaaaccgggc ttggacgccg cattgcgctg attctggtga
    14821 aaaagatggg gcatcgcacg ctgtttctcg gctatgcggt gatgttctcc gagctgatcc
    14881 tagcacctgt aacaccgtcc aactcggcgc gtggtgcggg tattatctat cccatcatcc
    14941 gtaacctgcc accgctctat caatcacaac caaacgacag cagttcgcgc agcattggct
    15001 cgtacatcat gtggatgggg attgttgccg actgcgtgac cagcgccatt ttcctgacgg
    15061 cgatggcacc taacttgctg ttaattggac tgatgaaaag cgcatctcac gccacgctga
    15121 gttggggcga ctggttcctc gggatgttgc cgctcagcat tttactgctt ctgctggttc
    15181 cctggctggc ttacgtgcta tacccgccgg tactgaagtc tggtgatcag gtgccgcgct
    15241 gggcagagac ggaactgcag gcaatgggcc cgctctgttc gcgtgaaaaa cggatgctgg
    15301 ggctgatggt aggcgcgctg gtgctgtgga ttttcggcgg tgattatatc gatgccgcga
    15361 tggtcggtta cagcgtggtg gcgctgatgc tgcttctgcg cattatcagt tgggacgaca
    15421 ttgtcagtaa taaagcggca tggaacgttt tcttctggct ggcctcgctt atcaccctcg
    15481 cgaccggact caacaatacc ggttttatta gctggtttgg caaactgtta gcaggcagct
    15541 taagtggtta tccgccgacg atggtgatgg tgacgttgat tgtggtgttt tatctactgc
    15601 gctacttttt cgccagcgcc acggcgtata cctccgccct cgcaccgatg atgatcgccg
    15661 ccgcgctggc gatgccggaa atcccgctgc cggtgttctg cctgatggtt ggcgcggcaa
    15721 ttggtctggg gagcattctt acaccatacg ccaccggacc cagtccgatt tactacggta
    15781 gtggttatct gccaacggcg gattactggc gactgggggc gatttttggg ctgatattcc
    15841 tcgtgttgct ggtgattacc ggcttactgt ggatgcccgt ggtgttgctt taagtgagga
    15901 cggtgccgga aattgctctc cggcacccaa tagttttacg cagccggtaa ctccgccagc
    15961 ggccagcgcg gacgcacgct aacgccgaga tccgccgtcg cgcctgcttt aaaccgcacc
    16021 attccggcat aggcgatcat cgcgccgtta tcagtacaaa attccggacg cgcgtagaac
    16081 acttcgccgc ggcgtttttt catcatttca gccagcttcg cccgtaacgt gcggttagca
    16141 ctcacgccgc ccgccatgac cagtcgctta aagcccgtct gatccaacgc tcgcttacac
    16201 ttaatcatca acgtatcgac caccgcatct tcaaaggcgc gggcgatatc agcacgcgtc
    16261 tggtcgtcgg tgctgttgtc acgaatcgtg ttcgccgcaa aggttttcag accagaaaag
    16321 ctgaaatcca gccccggacg gtcggtcatc ggacgcggga aaacaaagcg cccggcagta
    16381 ccctgagccg ccattttcga cagtaacggt ccgccaggat aatccagccc cagcagcttc
    16441 gcggttttat caaacgcttc accggcggca tcatcgatag actcgccgag cagctcgtac
    16501 tgaccaatgc cagtcacgct gattaactgc gtatgaccgc cggacaccag cagcgcgaca
    16561 aacggaaatt ccggcgggtt atcttccagc atcggcgcta acagatgccc ttccatatgg
    16621 tgtaccggga ttgccggaac gttccaggca aacgccagag agcgccccac ggttgcgcca
    16681 accagcagtg cgccgactaa tccagggcct gcggtatagg ccacagcatc aatgtctttt
    16741 gccgttaaac cagactcctt tagcgccgcc tggatcaacg gtacggtttt acgcacatga
    16801 tcgcgggagg ccagttcagg cacgacgccg ccgtagtcag cgtgcaattt cacctgacta
    16861 tacaattggt tggctaacaa acctttttca tcgtcgtaaa tggcgatgcc ggtttcatcg
    16921 caggaagttt caatacccag tacacgcatg acttgtttta cctcgcttta ttaccgcgca
    16981 gtgtaggacc aatgcgggtt gatgtaaaac tttgttcgcc cctggagaaa gcctcgtgta
    17041 tactcctcac ccttataaaa gtccctttca aaaaaggccg cggtgcttta caaagcagca
    17101 gcaattgcag taaaattccg caccattttg aaataagctg gcgttgatgc cagcggcaaa
    17161 ccgaattaat caaaggtgag aggcacatgc cggtaattaa agtacgtgaa aacgagccgt
    17221 tcgacgtagc tctgcgtcgc ttcaagcgtt cctgcgaaaa agcaggtgtt ctggcggaag
    17281 ttcgtcgtcg tgagttctat gaaaaaccga ctaccgaacg taagcgcgct aaagcttctg
    17341 cagtgaaacg tcacgcgaag aaactggctc gcgaaaacgc acgccgcact cgtctgtact
    17401 aattccccga gagcgttgct ctccgatcag accgagttgt agttgtaagg ccgtgcttcc
    17461 gaaaggaatg cgcggcttat tttcgtttat gaattgctaa aaatcggggc ctatggctgg
    17521 acgaatccca cgcgtattca ttaatgatct gctggcacgc actgacatcg tcgatctgat
    17581 cgatgcccgt gtgaagctga aaaagcaggg caagaatttc cacgcgtgtt gtccattcca
    17641 caacgagaaa accccgtcat tcaccgttaa cggtgagaaa cagttttacc actgctttgg
    17701 atgtggcgcg cacggcaacg cgatcgactt cctgatgaac tacgacaagc tcgagttcgt
    17761 cgaaacggtc gaagagctgg cagcaatgca caatcttgaa gtgccatttg aagcaggcag
    17821 cggccccagc cagatcgagc gccatcagag gcaaacgctt tatcagttga tggacggtct
    17881 gaatacgttt taccaacaat ctttacaaca acctgttgcc acgtctgcgc gccagtatct
    17941 ggaaaaacgc ggattaagcc acgaggttat cgctcgcttt gcgattggtt ttgcgccccc
    18001 cggctgggac aacgtcctga agcggtttgg cggcaatcca gaaaatcgcc agtcattgat
    18061 tgatgcgggg atgttggtca ctaacgatca gggacgcagt tacgatcgtt tccgcgaacg
    18121 ggtgatattc cccattcgcg ataaacgcgg tcgggtgatt ggttttggcg ggcgcgtgct
    18181 gggcaacgat acccccaaat acctgaactc gccggaaaca gacattttcc ataaaggccg
    18241 ccagctttac ggtctttatg aagcgcagca ggataacgct gaacccaatc gtctgcttgt
    18301 ggtcgaaggc tatatggacg tggtggcgct ggcgcaatac ggcattaatt acgccgttgc
    18361 gtcgttaggt acgtcaacca ccgccgatca catacaactg ttgttccgcg cgaccaacaa
    18421 tgtcatttgc tgttatgacg gcgaccgtgc aggccgcgat gccgcatggc gagcgctgga
    18481 aacggcgctg ccttacatga cagacggccg tcagctacgc tttatgtttt tgccggatgg
    18541 cgaagaccct gacacgctgg tacgaaaaga aggtaaagaa gcgtttgaag cgcggatgga
    18601 gcaggcgatg ccactctccg catttctgtt taacagtctg atgccgcaag ttgatctgag
    18661 tacccctgac gggcgcgcac gtttgagtac gctggcactg ccattgatat cgcaagtgcc
    18721 gggcgaaacg ctgcgaatat atcttcgtca ggaattaggc aacaaattag gcatacttga
    18781 tgacagccag cttgaacgat taatgccaaa agcggcagag agcggcgttt ctcgccctgt
    18841 tccgcagcta aaacgcacga ccatgcgtat acttataggg ttgctggtgc aaaatccaga
    18901 attagcgacg ttggtcccgc cgcttgagaa tctggatgaa aataagctcc ctggacttgg
    18961 cttattcaga gaactggtca acacttgtct ctcccagcca ggtctgacct ccgggcaact
    19021 tttagagcac tatcgtggta caaataatgc tgccaccctt gaaaaactgt cgatgtggga
    19081 cgatatagca gataagaata ttgctgagca aaccttcacc gactcactca accatatgtt
    19141 tgattcgctg cttgaactgc gccaggaaga gttaatcgct cgtgagcgca cgcatggttt
    19201 aagcaacgaa gaacgcctgg agctctggac attaaaccag gagctggcga aaaagtgatt
    19261 taacggctta agtgccgaag agcgatcggg aagcccccga cagccgcact gagaggcagc
    19321 ggcaaatata taagtacgcc ctcgtaatta tcgttggcgg taaacaaccg ttggatttca
    19381 gcgttaacgg ctgaaggaca tcgggtcaat cgcccaacac caacctcatg aaataagtgt
    19441 ggataccgtc ttatggagca aaacccgcag tcacagctga aacttcttgt cacccgtggt
    19501 aaggagcaag gctatctgac ctatgccgag gtcaatgacc atctgccgga agatatcgtc
    19561 gattcagatc agatcgaaga catcatccaa atgatcaacg acatgggcat tcaggtgatg
    19621 gaagaagcac cggatgccga tgatctgatg ctggctgaaa acaccgcgga cgaagatgct
    19681 gccgaagccg ccgcgcaggt gctttccagc gtggaatctg aaatcgggcg cacgactgac
    19741 ccggtacgca tgtacatgcg tgaaatgggc accgttgaac tgttgacccg cgaaggcgaa
    19801 attgacatcg ctaagcgtat tgaagacggg atcaaccaag ttcaatgctc cgttgctgaa
    19861 tatccggaag cgatcaccta tctgctggaa cagtacgatc gtgttgaagc agaagaagcg
    19921 cgtctgtccg atctgatcac cggctttgtt gacccgaacg cagaagaaga tctggcacct
    19981 accgccactc acgtcggttc tgagctttcc caggaagatc tggacgatga cgaagatgaa
    20041 gacgaagaag atggcgatga cgacagcgcc gatgatgaca acagcatcga cccggaactg
    20101 gctcgcgaaa aatttgcgga actacgcgct cagtacgttg taacgcgtga caccatcaaa
    20161 gcgaaaggtc gcagtcacgc tgccgctcag gaagagatcc tgaaactgtc tgaagtattc
    20221 aaacagttcc gcctggtgcc gaagcagttt gactacctgg tcaacagcat gcgcgtcatg
    20281 atggaccgcg ttcgtacgca agaacgtctg atcatgaagc tctgcgttga gcagtgcaaa
    20341 atgccgaaga aaaacttcat taccctgttt accggcaacg aaaccagcga tacctggttc
    20401 aacgcggcaa ttgcgatgaa caagccgtgg tcggaaaaac tgcacgatgt ctctgaagaa
    20461 gtgcatcgcg ccctgcaaaa actgcagcag attgaagaag aaaccggcct gaccatcgag
    20521 caggttaaag atatcaaccg tcgtatgtcc atcggtgaag cgaaagcccg ccgtgcgaag
    20581 aaagagatgg ttgaagcgaa cttacgtctg gttatttcta tcgccaagaa atacaccaac
    20641 cgtggcttgc agttccttga cctgattcag gaaggtaaca tcggcctgat gaaagcggtt
    20701 gataaattcg aataccgccg tggttacaag ttctccacct acgcaacctg gtggatccgt
    20761 caggcgatca cccgttctat cgcggatcag gcgcgcacca tccgtattcc ggtgcatatg
    20821 attgagacta tcaacaaact caaccgtatt tctcgccaga tgctgcaaga gatgggccgt
    20881 gaaccgacgc cggaagaact ggctgaacgt atgctgatgc cggaagacaa gatccgcaaa
    20941 gtgctgaaga tcgccaaaga gccaatctcc atggaaacgc cgatcggtga tgatgaagat
    21001 tcgcatctgg gggatttcat cgaggatacc accctcgagc tgccgctgga ttctgcgacc
    21061 accgaaagcc tgcgtgcggc aacgcacgac gtgctggctg gcctgaccgc gcgtgaagca
    21121 aaagttctgc gtatgcgttt cggtatcgat atgaacaccg accacacgct ggaagaagtg
    21181 ggtaaacagt tcgacgttac ccgcgaacgt atccgtcaga tcgaagcgaa ggcgctgcgc
    21241 aaactgcgtc acccgagccg ttctgaagtg ttgcgtagct tcctggacga ttaatcggta
    21301 ggccggatca ggcgttacgc cgcacccggc actagtctct ctgcacaaac gccaccttt
//
LOCUS       DAJTYG010000064        21220 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTYG010000064 DAJTYG010000000
VERSION     DAJTYG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21220
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.064"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(35..1015)
                     /locus_tag="OO568_002569"
     CDS             complement(35..1015)
                     /locus_tag="OO568_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6641880.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSALDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1023..1733)
                     /locus_tag="OO568_002570"
     CDS             complement(1023..1733)
                     /locus_tag="OO568_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR6641881.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1950..3773)
                     /gene="typA"
                     /locus_tag="OO568_002571"
     CDS             complement(1950..3773)
                     /gene="typA"
                     /locus_tag="OO568_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6641882.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4146..5555
                     /gene="glnA"
                     /locus_tag="OO568_002572"
     CDS             4146..5555
                     /gene="glnA"
                     /locus_tag="OO568_002572"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6641883.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5728..6777
                     /gene="glnL"
                     /locus_tag="OO568_002573"
     CDS             5728..6777
                     /gene="glnL"
                     /locus_tag="OO568_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6641884.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6789..8198
                     /gene="glnG"
                     /locus_tag="OO568_002574"
     CDS             6789..8198
                     /gene="glnG"
                     /locus_tag="OO568_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6641885.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8310..8420
                     /locus_tag="OO568_002575"
     CDS             8310..8420
                     /locus_tag="OO568_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6641886.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8649..10022)
                     /gene="hemN"
                     /locus_tag="OO568_002576"
     CDS             complement(8649..10022)
                     /gene="hemN"
                     /locus_tag="OO568_002576"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6641887.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10211..10720)
                     /gene="yihI"
                     /locus_tag="OO568_002577"
     CDS             complement(10211..10720)
                     /gene="yihI"
                     /locus_tag="OO568_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6641888.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11302..11934
                     /locus_tag="OO568_002578"
     CDS             11302..11934
                     /locus_tag="OO568_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6641889.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12316..15102)
                     /gene="polA"
                     /locus_tag="OO568_002579"
     CDS             complement(12316..15102)
                     /gene="polA"
                     /locus_tag="OO568_002579"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6641890.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15466..16398
                     /locus_tag="OO568_002580"
     CDS             15466..16398
                     /locus_tag="OO568_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6641891.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLISSQRQNK"
     gene            complement(16439..17869)
                     /locus_tag="OO568_002581"
     CDS             complement(16439..17869)
                     /locus_tag="OO568_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6641892.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRIQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNVVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18024..18650)
                     /gene="dsbA"
                     /locus_tag="OO568_002582"
     CDS             complement(18024..18650)
                     /gene="dsbA"
                     /locus_tag="OO568_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6641893.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(18667..19653)
                     /gene="srkA"
                     /locus_tag="OO568_002583"
     CDS             complement(18667..19653)
                     /gene="srkA"
                     /locus_tag="OO568_002583"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6641894.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQESPLQLTPMY"
     gene            complement(19730..19999)
                     /locus_tag="OO568_002584"
     CDS             complement(19730..19999)
                     /locus_tag="OO568_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6641895.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20069..20653
                     /gene="mobA"
                     /locus_tag="OO568_002585"
     CDS             20069..20653
                     /gene="mobA"
                     /locus_tag="OO568_002585"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6641896.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20635..21162
                     /gene="mobB"
                     /locus_tag="OO568_002586"
     CDS             20635..21162
                     /gene="mobB"
                     /locus_tag="OO568_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6641897.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDAAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5174 a         5340 c         5114 g         5592 t
ORIGIN      
        1 atggttttat taactgcgta tgagttaaaa cttattagtg gttcagaaga tggtttgcct
       61 cttgtttgat ttgttgtagt acgttacgca catgatttat ctgattaatt gcatcttctt
      121 tttctttacg taaccgcgca tccagtaaat gattttctgg taggggtgtt tcactcatct
      181 ggcgataagg gatccacgga ttatcatctt cgtcttcaaa gcggaacgcc ggcgcatagt
      241 aatcaacctc ttcttccagg ccatatgccg tcacctgcgg ctcatcatca ccaaggcaga
      301 taaggtgcgt taataacgct ttgaatggca tatcaccctg acctgaaata caggctttat
      361 gacccaggcc accctgttct ttaacgatca aggcatcttt aatatggacc tgggtaatat
      421 gcggtgccat cgtttttaag gcgtcgatgg gatgctcatt tgcattgatc atgttagcaa
      481 aatcaaacag taaggaaaga gactccatct cactttcttt gaccagcgac accagctcat
      541 gactttttaa atcttcatgc tgctcaatcg taaaagtcag gccgctgtcc tgatacgttt
      601 cccgtacata ggcaatgtcg ttagcgataa tcgataatac gtcgcgcaaa ttaccttcat
      661 aacgtggata aaaacgtacg gacgttgccc cagttttcaa cgcgatggcg acggcttcgt
      721 caatagatgc cttatctaaa gcgctggttt caatatgaat atcaaggttc agacggcggg
      781 ctttatcccc aaaggcagag agttctttat cgtccatatt accaagagaa aaacgctcgc
      841 catccagaac atggattttg acgccacgta gattattttc acttgcgata tcaagtaaat
      901 cagcaggcaa cactctttcc agccgcatat ttaaatgaaa ggcataagca tgtaaataaa
      961 gaggaagagt gtcgacacgt tgtagaatct ttcttgcgtt atttattgtc accataaaac
     1021 acctacagat taatgatgag gtctacggcc tcaaatcgcc aatattcaat atctatctgg
     1081 acaatattgc cttgctggtc ggcgcgatgc ttttcaatta agatcgctgg catacccgca
     1141 gaaccgccaa gatacttgct gatatcgccc ggcattctga cgggtttgaa aatcaatttt
     1201 tttactaccg tctctttttg gtagcacttt tcccagactg cagaaaatga gtggttttca
     1261 agttgttcaa taaaacccgg agcaacttca ggattaatat atgtttcgtg atagaaaact
     1321 ttatgtcctt ccagcgcccc ccagccggtg atgcgataga gttcagttga tggcgtcact
     1381 gcaatcagtg gcgcgagcgt ctcgggaata tcgctggtac ggtttttctc ggtaaacccc
     1441 caagaaggct ctcgcccttg ctcaattgcg gcccgctgaa agctcgccga cagctccgga
     1501 ctgtaattaa atcgcggctg ggtcacgaac caccccttac gatccttacg gaagattttg
     1561 gattctgcct cgaggttcaa caacgcctgg cgcagcgtca tacgtttaat gcccagcagt
     1621 tctcccagct ctctttctga agggagtttt ccacccggcg tggtgatgcc atctttcaac
     1681 caccgatcca gtttctcttt tgcattttct acggtggatt gattctccgc cattgtttaa
     1741 tccttttggt ttaaaccaga tgaagcatct tacgcttacg ccaaaaaaga aagcaattaa
     1801 cggtgtatga ctttgcagat aattgtgaca aagctctcta ttgacgtaat cacagactta
     1861 taagggagat actcgcattg gtgtgcgctc acgacaggta aaaaaaaacc tgccatcgct
     1921 ggcaggtttt ttatgactaa aaaacgaaat taatcgtctt tcggtgcgcg gttggcgcgg
     1981 cgacgatcgt tttccgtcag gtgacgttta cgaatacgga tagaggtcgg agtcacttct
     2041 accagttcgt catcatcgat gaactccaga gcttgttcca gagtcatgcg gataggcgga
     2101 accagaacaa cagcttcgtc agtaccggaa gcacgcatgt tggtcagttt cttaccggtc
     2161 aggcagttta cagtcaggtc gttagagcgg ctatgaatac cgataatctg accttcgtaa
     2221 acttctgcac cgtgaccgag gaacagctta ccgcgatcct gcagaccgaa cagcgcgaac
     2281 gcgacagctt taccctgacc gttagagatc agtacaccgt tctgacgctg acccacttcg
     2341 cccggacgta cgtcgtcgta gtggctgaag gtggagtaca gcagaccagt accggaagtc
     2401 atggtcatga actcagaacg gaagccaatc agaccacggc ttgggatcac gtagtcgaga
     2461 cgtacgcggc ctttaccgtc tggattcatg tttttcaggt cgcctttacg ttcgcccagc
     2521 gcctgcatca cagaaccctg atgctgttct tcaacgtcca gcgtcacgtt ttcatacggc
     2581 tcttgtttac gaccgtcgat ttcacggaag attactttcg gacgggatac cgccagttcg
     2641 aaaccttcac gacgcatgtt ttcgataaga acagagaggt gcagttcacc acgaccagaa
     2701 acgcggaacg catcggcgtc ttcggtttct tctacgcgca gcgcaacgtt gtgtaccagt
     2761 tctttgttca gacgatcgag gatctgacga gacgttacga acttaccttc tttaccgcag
     2821 aacggcgagg tgttaacgca gaagaacata gaaacggtcg gctcatcaac ggagagtgcc
     2881 ggcagcgctt caacgttttg cgtgtcgcaa acggtgtcag aaatgttcag ttcgcccagg
     2941 cccgtgatcg ccacgatatc gccagcttcc gccagatcgg tttcgatacg ttccagaccg
     3001 aggtggccca gcactttacc gactttcgcg ttacgggttt tgccttcgct atcgatgata
     3061 gtaacctgct ggttcggctt cactttacca cgcttgatgc ggccaatgcc gataacgcca
     3121 acatagctgt tgtaatcgag ctgagaaatc tgcatctgga acggaccgtc aaggtcaacg
     3181 tccggcgcag gaacgtggtc aacaatcgcc tgatacagcg gggtcatgtc ttccgccata
     3241 tcttcgtggt ccagacccgc gataccgttc agcgcagaag cgtaaacgat cgggaagtcc
     3301 agctgctcgt cggtcgcgtc gaggttaacg aacagatcga atacctgatc cacaacccaa
     3361 tcaggacgcg cgccagggcg gtcaactttg ttgataacaa caatcggctt caggccgtaa
     3421 gcaaacgctt ttttggttac gaagcgcgtt tgcggcatcg ggccgtcaaa tgcgtcaacc
     3481 accagcagca ctgagtctac catggacatt acacgttcaa cttcaccacc gaagtcggcg
     3541 tgccccgggg tatcaacgat gttgatacgg taatcattcc atttgatagc ggtgtttttc
     3601 gcgaggatgg taatcccacg ctctttctcc aaatcgttgg agtccatcac gcgctcttgg
     3661 gtttcggcac gagagtcgaa cgtaccggat tgttggagca gcttgtctac caaggtggtt
     3721 ttaccatggt ctacgtgcgc gatgatggcg atattacgca atttttcgat cacaactttg
     3781 cctcaggcat tagaaatagc gcgttattgt acacggatta atcgcactac aaaacaggat
     3841 cacaaacatc ctccgcaaac aagtattgca gagtcccttt gtgatcgctt tcacggagca
     3901 taaaaagggt tatccaaagg tcattgcacc aatatggtgc ttaatgtttc cattgaagca
     3961 ctatattggt gcaacattca catcatggtg cagccctttt gcacgatgtt aagcatgata
     4021 acgcctttta ggggcaattt aaaagttggc acagatttcg ctttatcttt tttacggcga
     4081 cacggccaaa ataattgcag atttcgttac cacgacgaca atgaccaatc caggagagtt
     4141 aaagtatgtc cgctgaacac gtactgacga tgctgaacga gcacgaagtg aagtttgttg
     4201 atttgcgctt caccgatacc aaaggtaaag aacaacacgt cactatccct gctcatcagg
     4261 tgaatgctga attcttcgaa gaaggcaaaa tgtttgacgg ctcctcgatt ggcggctgga
     4321 aaggcattaa cgaatccgac atggtgctga tgccagacgc atccaccgca gtgattgacc
     4381 cgttcttcgc cgactccacc ctgattatcc gttgcgacat ccttgaacct ggcaccctgc
     4441 aaggctatga ccgtgacccg cgctctattg cgaagcgcgc cgaagattac ctgcgttcca
     4501 ctggcattgc cgacaccgta ctgttcgggc cagaacctga attcttcctg ttcgatgaca
     4561 tccgtttcgg atcatctatc tccggttccc acgttgctat cgacgatatc gaaggcgcat
     4621 ggaactcctc cacccaatac gaaggtggta acaaaggtca ccgtccggca gtgaaaggcg
     4681 gttacttccc ggttccgccg gtagactctg ctcaggatat tcgttctgaa atgtgtctgg
     4741 tgatggaaca gatgggtctg gtggttgaag cccatcacca cgaagtagcg actgctggtc
     4801 agaacgaagt ggctacccgc ttcaatacca tgaccaaaaa agctgacgaa attcagatct
     4861 acaaatatgt cgtgcataac gtagcgcacc gcttcggtaa aaccgcgacc tttatgccaa
     4921 aaccgatgtt cggtgataac ggctccggta tgcactgcca catgtctctg tctaaaaacg
     4981 gcgttaacct gttcgcaggc gacaaatacg caggtctgtc tgagcaggcg ctgtactaca
     5041 ttggcggcgt aatcaaacac gctaaagcga ttaacgccct ggcaaacccg accaccaact
     5101 cttataagcg tctggtcccg ggctacgaag caccggtaat gctggcttac tctgcgcgta
     5161 accgttctgc gtctatccgt attccggtgg tttcttctcc gaaagcacgt cgtatcgaag
     5221 tacgtttccc ggatccggca gctaacccgt acctgtgctt tgctgccctg ctgatggccg
     5281 gtcttgatgg tatcaagaac aagatccatc cgggcgaagc catggacaaa aacctgtatg
     5341 acctgccgcc agaagaagcg aaagagatcc cacaggttgc aggctctctg gaagaagcac
     5401 tgaacgaact ggatctggac cgcgagttcc tgaaagccgg tggcgtgttc acggatgaag
     5461 cgatcgatgc gtacatcgct ctgcgtcgcg aagaagatga ccgcgtgcgt atgactccgc
     5521 atccggtaga gtttgagctg tactacagcg tctaatagtt gaagttgtac tacccggcgc
     5581 acaacgccgg gatttagttg ccgtggaaac tttcagccca tctctgcatg ggcttttttc
     5641 tccgtcaatt ctctgatgct tcgcgctttt tatccgtaaa aagctataat gcactaaaat
     5701 ggtgcaacct gttcaggaga ctgctttatg gcaacaggca cgcagcccga tgctgggcag
     5761 atcctcaact cgctgattaa cagtattttg ttaattgatg acaatctggc gatccattac
     5821 gccaaccccg ccgcgcaaca actgctcgcc caaagctccc gcaaattgtt tggtacgccg
     5881 ttaccggaac tgttgagcta cttctcatta aatatcgagc tgatgcaaga aagtctggag
     5941 gcggggcaag gctttaccga taacgaagtg acgctggtca tcgatggtcg ctcgcatatc
     6001 ctttctgtga cggcccagcg tatgccggac ggcatgatcc tgctggagat ggcaccgatg
     6061 gataaccagc gccgcttaag ccaggaacaa ctacaacatg ctcaacaagt agctgcccgt
     6121 gatttagtgc gcggcctggc acatgagatt aaaaatccgc ttggcggttt acgtggcgcg
     6181 gcgcagttac tcagcaaagc gttacctgac ccatcactac tcgaatatac caaagtgatt
     6241 atcgaacagg cggaccggct gcgaaatttg gtcgaccgtc tgttggggcc gcagctgccc
     6301 ggtacgcgcg ttaccgaaag tattcacaaa gtggctgaac gcgtggtaac gctggtgtcg
     6361 atggaactgc cggacaacgt gcggttgatt cgtgattacg atcccagcct gccggaactg
     6421 gcgcacgacc cggatcaaat tgaacaggtc ttgctgaata ttgtgcgcaa tgcgctacag
     6481 gcgctggggc cggaaggcgg tgaaatcatt ctgcgtaccc gcaccgcgtt tcaactgacc
     6541 ttacacggcg agcgctaccg gctggcggcg cggattgatg tggaagataa cgggccgggc
     6601 attccgcctc atttgcagga tacgctgttt tacccgatgg tcagcggccg cgaaggtggc
     6661 accgggcttg gcttatccat cgcccgtaat ttgattgatc agcattcagg caaaattgaa
     6721 tttaccagtt ggccaggtca taccgagttc tcggtttacc tgcctatcag gaaataaagg
     6781 tgacgtttat gcaacgaggg atagtctggg tagtcgatga cgatagttcc atccgttggg
     6841 tgcttgaacg tgcgctcgct ggagcgggtt taacctgtac gacatttgag aacggcgcgg
     6901 aagtactgga ggcgctggcg agcaaaacgc cggatgtgct gctttcagat atccgtatgc
     6961 cgggaatgga cgggctggcg ctgctcaagc agattaaaca gcgccatcca atgcttccgg
     7021 tcatcattat gaccgcacat tccgatctgg atgctgccgt cagcgcctat caacaagggg
     7081 cgtttgatta tctgcccaaa ccgtttgata tcgacgaagc agtggcgctg gttgagcgcg
     7141 ctatcagtca ttaccaggaa cagcagcagc cgcgtaatat tcagcttaac ggcccaacga
     7201 ccgatatcat cggcgaagcg caagccatgc aggacgtgtt ccgtattatc ggtcggcttt
     7261 cgcgttcttc tattagcgtg ctgattaacg gcgaatccgg caccggtaaa gaactggtcg
     7321 ctcatgccct gcatcgccac agtccgcgca ccaaagcgcc gtttatcgcg ctgaatatgg
     7381 cagctatccc aaaagatttg atcgaatcag aactgtttgg ccacgagaaa ggcgcgttta
     7441 ctggcgcgaa taccattcgt caggggcgtt ttgaacaggc cgatggcggc acgttatttc
     7501 tcgacgaaat tggagatatg ccgctggatg tgcagacgcg tttgctgcgc gtgctggcag
     7561 acggtcagtt ttatcgcgtt ggcggctatg cgccggtgaa agtggatgtg cggattatcg
     7621 ctgccactca ccagaatctt gaacagcggg tgcaggaagg taagttccgt gaggatctgt
     7681 tccaccgcct gaacgttatc cgcgttcatc tgccgccgct gcgcgaacgt cgggaagata
     7741 ttccccgtct ggcgcgccat tttttacagg ttgccgcgcg cgaactgggc gtagaagcga
     7801 agttgctgca tccggaaacc gaagctgctc tgacgcgtct ggcgtggcca ggcaacgtgc
     7861 gccagctgga aaacacctgc cgctggctaa cggtgatggc cgccgggcag gaagtgttga
     7921 ttcaggattt gcccggcgaa ctgtttgaat caacggttgc ggagagtact tcgcaaatgc
     7981 aaccggacag ttgggcgaca cttttagcgc agtgggcaga cagagcgctg cgttccggtc
     8041 atcaaaatct gctttccgaa gcgcagccag agctggagcg gacgttactg acgaccgcgt
     8101 tgcgacatac gcaggggcat aaacaggaag cggcgcggct actcggctgg ggccgcaaca
     8161 ctctgacgcg taagttaaaa gagctgggga tggagtgatt cacggcttgt gtgtaaagat
     8221 tgattattga gcgcaaattg ctggtatttt gcgctttact gttccgataa gttcagtatg
     8281 atcttgcccg gaaaacgggg agagtcatta tgctggaatc aataattaat ctggtatcga
     8341 gtggcgcagt tgacagccac acaccgcaaa ctgccgttgc tgccgtgctg tgtgccgcga
     8401 tgattgggct gtttagctga agctcgttgt ttatgccgga tgcggagaaa gtcgtaggcc
     8461 tgataagacg cgaccagcgt cgcatcaggc atcatgctca gatgcctgat gcggcgtaaa
     8521 cgccttatcc ggcctacaaa agcaataatt tcaacaggtt ttgttgccca gcgcatcagg
     8581 caatcttgtt ctcagcacat tgatggcgct ttcgttttct actttgtaaa cgaagcgcca
     8641 ttcactcttt aaatcacccg tgagaactgc tgcatccgcg ctttctggcg cagataggta
     8701 tcaaagcaca tgcaaatgtt gcggatcagc aagcgacctt tcgccgtcac ctgtattccc
     8761 ttctcatcca catccaccag cccatctttt gctaacgggg cgagcagctt gagatcttcc
     8821 gcaaagtaat cagcgaagtg caaatcccac tgtttctcaa gaggggcgta atccagacgg
     8881 aagttgcaga tgagcgactt aatcacatcg cggcgaatac agtcatcacg cgttagcgca
     8941 ataccacgcc acagcgcatt gccttgttca tccacttgct gatagtactg cttcaactct
     9001 ttctggttct gcgcgtagca gtcgccaatc atgctgatgg cggaaacgcc catccccagc
     9061 agatcggtat cgccctgagt ggtgtagccc tggaagttac gatgcagcac gccttcacgc
     9121 tgggccaccg ccagctcgtc atcaggacgg gcaaagtgat ccataccgat aaactgatag
     9181 cccgattgcg tcaggaaggc gatggtttcc tgcaggatat cgagtttttg ctgcggactc
     9241 ggcaggtcag catctttgat tttgcgctga gcagcaaaaa tggtcggcag atgcgcgtag
     9301 ttaaaaacgc tcagacgatc ggggttcagc tccgccacac gtttcagggt aaaggcgaaa
     9361 ctctccggcg tctgtttcgg caggccgtaa atcaggtcga tgttggtgga ggtaaaacca
     9421 atctcacgcg catggttaag cagtgcaaag atgaactctt catcctgctc gcggttaacc
     9481 agacgttgca cttctttgtt gaagtcctgc acgcccatgc tcaggcgatt aaagccttcg
     9541 gcgcgtaaat gatcgagtac atccagttcg atttcccgcg gatcgacttc gatcgaaatc
     9601 tccgcatcgg tattgaaatg gaagttttcg cgcagcagct tcatcagacg gctgatttgc
     9661 gctttattca gatacgtcgg cgttccgccg ccccagtgca attggctgac gtgacgcccg
     9721 gcgaacagcg gtgcacgatg gacgatttct tgctccagcg cgtccagata ctgatcggcc
     9781 ttgtgctgct ggcgagtaac aatcttattg caaccgcaga agtaacaaag cttatggcag
     9841 aacgggatat gtacgtagag agataatgga cgctcaggat agcgcgccac ggcttgtaaa
     9901 aacgcctgtt cgccgaagtc ttctgaaaac tccagcgcgg tcgggtacga ggtgtatcgt
     9961 ggcccggaat agttatattt ctggatcagg gccagatccc agtcgatttg ctgtacagac
    10021 atgctcactc cttccgatgg cgtctctggc ggctacggcg taccggcgga tgcccgtttc
    10081 gggtcatcag ccggttgcgc agccactttt gtcgccgcga caaccgctgt agtttaacga
    10141 ataaccacac cagataacat ataaccggaa gggttatcag cagaacggga aggcccacgg
    10201 cggcaatccg ttagttgccc cgcagcagac gcatcatgtc ttcttgcttc tcgtcttctt
    10261 cctcttcttc gtcatcgtca taagagaggc cgagtttctg catcaactca tcaatacgat
    10321 ccagtttagc atccacccag gattgctctt cggcgctcag ggtttcgcct gcttccagac
    10381 gttccagcag cgcatccaga cgctcatccg tttccagtaa ttccaactcc gcctgcggtg
    10441 aaagcatagg tttctcactc ttcggtttgt gctgtttggt gactttttca gtcactccca
    10501 atggaatagg ggttttactg ccaatacgtg gatcttttgg tgcgttctgg cctttgctgc
    10561 ctgacgtggt gtttccgccc gctgcgcggc tgcccggcgc atgaccacga cgttttttct
    10621 gacgcttacg gtcacgagct tcctgatcca gctcctcgcg tgtttttcga cgcgcttttg
    10681 catgaccttt gctgcgtgag tttgaagatg atggtttcat aatggttcgt cttttagccg
    10741 ttttattcag tatagatttg cggcggaatc taacagaaag caagcaaaga aaaaaggcga
    10801 cagattactc tgtcgccttt tttcctgact cataaccctt aacgggtctt acaatccttg
    10861 caggccgcca acacaccctg tttttccatt agccggaaca atccgtgttg attccatttc
    10921 cgtttaatta cgtctccgga cgtttgtctt cctgacaatc ctctgtcttc gcctcctggc
    10981 gctcctgacc ctcatcctga gtcattgttc ctgttagcgt cctcgctcta tgggtgctac
    11041 tttacgcctt tgctttaaac aaacaatcaa ccgccatcag tatttgcaca ttttcagagt
    11101 tttatataaa agcaattcac tgatttatat agcttatttg acatgcatcg cagaattttc
    11161 tctgctattt cgcataaaga ctatcggcaa tgtctcacaa agcgtatggc ttttacttac
    11221 aacgctcacg ccgaaaaatc cgccttttat tatggttcag gtataatcgc cacaaagctt
    11281 tgactgacgg agacgaccgc tttgactaat ttgaattatc aacagacgca ttttgtgatg
    11341 agtgcgcctg atattcgcca cctaccttcc gataccggaa ttgaagtggc ttttgcaggc
    11401 cgttccaacg caggtaaatc cagcgcgctg aacacgctga ctaaccagaa aagcctggct
    11461 cgtacctcaa aaaccccagg gcgcacccag cttatcaacc tgtttgaagt ggctgacggc
    11521 aagcgtctgg ttgacttgcc tgggtacggt tatgcggaag tcccggaaga gatgaagcgc
    11581 aaatggcagc gtgcgctcgg cgaatacctc gaaaaacgtc agagcctgca aggtctggtg
    11641 gtgctaatgg atattcgcca tccgctgaaa gatttggatc agcagatgat tgagtgggcg
    11701 gtagacagca atatcgccgt tctggtgctg ctgaccaaag cggacaaact ggcaagcggc
    11761 gcacgtaaag cgctattgaa tatggtgcgt gaagctgtac tggcgtttaa cggtgatgtg
    11821 caggttgaaa cgttttcttc gttgaagaaa caaggcgtag acaagctgcg gcagaaactg
    11881 gatacctggt ttagcgagat gcagcctgta gaagaaacgc aggacggcga ataattttct
    11941 tgccttaatg cttgtgccgg atgtggcgta tccggcccgt aaattcaata gattacaatt
    12001 ctaccgttgg cctgataaac atagcgcatc aggcattacg gatcttttct ttcgcccaat
    12061 aaaaaacgcc ccagtcatta ctgactgggg cggctaaaat attcagccaa atccgattac
    12121 gtgaagtaaa aggtctgaaa gatagaacat cttacctctg taccctacgc gactaacttt
    12181 actctttttt gttcagttca gaaagcactt tttgtaattt tttttcagtt gttgcatagg
    12241 aatttctaat agccatcaca aaacgtgaca gcttatgttg cttacttacg aaaaaagggc
    12301 atgttcaggc gaatcttagt gcgcctgatc ccagttttcg ccactcccca cttccaccag
    12361 caacggcaca tccagacggg tacagttttc catcagttga tgaatctgct tcgcgacggc
    12421 atcaacatca tctttatgaa cttcaaatac cagttcatcg tgtacctgca tgatcatacg
    12481 tacacgcggt tgctcagcct gtaaccacgc atcaacggca atcatcgccc gtttgataat
    12541 gtcggcggcg gttccctgca ttggcgcgtt aatggctgca cgttcagccg ctgcacgacg
    12601 agcaccattg ctggatttga tatccggcag atacagacgg cgtccgtcca gcgtttcaac
    12661 gtagcccagc tctttcgcct gagcacgggt gcgttccata tactccagca cgccagggta
    12721 gcgttcgaag taaaggtcca tgtacttctg cgcttcttta cgtggaatgt tcaattgccg
    12781 cgccagaccg aaagcactca tgccataaat cagaccaaag ttgatcgctt tcgcgctacg
    12841 gcgttgctcg ctggtgacgg tttccagtgg caaaccaaac acttctgccg ccgttgcccg
    12901 gtggatatct tttccttccg cgaatgcggt cagcaagcct ttatcacgcg agagatgcgc
    12961 cataatgcgc agttcaatct gcgagtagtc cgctgagaca atcacataat cctctggcgc
    13021 aataaacgcc tggcggatac gacgaccttc ttcgttacgc accggaatgt tttgcaggtt
    13081 aggatcggtt gacgataaac gtcccgttgc agttactgcc tggtgataag aggtatgcac
    13141 acgcccggtt ttcgggttga tcatcagcgg cagcttgtcg gtgtaagtcg acttcagctt
    13201 cgccagacca cgatactcca gaatcacttt tggcaacgga tagtccagcg ccagttcttc
    13261 cagtacctct tccgacgttg acggcgcgcc acccggcgtt ttcttcagcg gtttaatacc
    13321 ctgtttttca aacagaatgg tttgcaactg cttggtggaa gaaaggttaa attcttcacc
    13381 tgcaatttca tgcgctttct tttccagctc agccagacgc aaggtgagct cttcagaatg
    13441 attgtgcagc actttcggat cgatcttcac accgttacgt tcaatgcgtg aaagcaccgg
    13501 caccagcggc atttcgatat tctcgaagac gttcaacggc cctttgtgtt tttgcagatc
    13561 cggccacatt ttcagatgca actgcaaggt gacatctgca tcttcggcgg cgtaacgtcc
    13621 ggcctcttcg agggcaatct ggttaaaggt cagttgattt ttgcctttac ccgcaatctc
    13681 ttcaaaagtg atggttttgt gcttcaacca acgttccgcg aggctgtcca tatcgtgacg
    13741 cccggcaacg ctattgagaa tgtaggactc cagcatggta tcaaacgcaa tcccacgcag
    13801 ttcaatgccg taattcgcca gaataccgcg atcgtatttc aggttttgcc cgaccttcag
    13861 cgccttttca tcttccagca gcggttttag caactcgagt gcacgctcgt gagagatttg
    13921 atcgggcgca tcaagataat catgagcaac cggaatatat gccgctacgc ctggctcgat
    13981 agcaaaagaa agcccgacca ggttagcaga gatgttatca aggctgtcgg tttcggtatc
    14041 aaatgcaaat accggcgctt tttccagctt cgcaatccac gctttcagtg tttcttcatc
    14101 aaggatagtg acgtagttgt cataagaaat caccgttgcc gtcacttctg gcgcttcgtc
    14161 tgcaacactg gtttcctgcg gcttcgcggc tggttttgcc cctttggcct gtaaccattt
    14221 gcccgcttcg acatcagcag tccagcgttt gaactcatac tttttgaaca gccccaacaa
    14281 ctcttccgct gccggttgct gcacttccag ttgttcacag gtcagctcca gttcaacatc
    14341 ggttttaatc gtcgccaact ggtatgagag ataagcaact tctttgtttt gctcgagctt
    14401 cgctgccatt gttttcgcgc cacggaagct caacccagca attttttctg gctcggcata
    14461 cagcgtatcc agcccgccaa gaccttgcag caatgcctgc gcggtttttt caccgacgcc
    14521 cggtacgcca ggaatgttat cagaggagtc acccatcagc gccaggaagt cgatgatcaa
    14581 ttctggcggc acgccgtact tattcaccac ctcttccggt ccgaggatgg tattcgtcat
    14641 ggtattgata agcgtaatat ttggcgtcac cagctgcgcc atatctttat cgccagtgct
    14701 aatcagcacc ggacgcccgg ctttttcggc ttcgcgcgcc agagtaccga taacgtcgtc
    14761 cgcttctacg ccagaaaccg ccagcagcgg cagtcccatc gctttaacca tcgcgtgcag
    14821 aggttcgatt tgtgctcgca gatcgtccgg cattggcggg cgatgtgatt tgtaatgttc
    14881 aaacagttca tcacgaaagg tttttccctt ggcgtcaaag accaccgctg catgcgtcgg
    14941 tttatattgc atgatcagac tgcgcagcat gttgaggaca ccatacatcg caccggtcgg
    15001 ctcgcctgcg ctgttagtca gcgggggaaa cgcgtgatat gcgcgataaa gataagatga
    15061 accatctaca aggataagtg gattttgggg gatctgaacc ataatgtccg tgcctgttta
    15121 tcagattatg ggtaaaggat gccacagaca ggatgaaaac atgagttttt caccgctttt
    15181 cgtgaaaatc ttttgtagat ctgctggatc gctcgcaaat cgtcatgtgg ataactttgt
    15241 gcataaatta tttacatcgc ttttaattta ttatcatgct ttttacagca tgatgtttgt
    15301 ctttattttt catgcagtta aaaataaatc catccctaat aattgagata atgggatttt
    15361 ttctaatgtg gatagatatg aattattttt ctccttaagg atcatccgtt atttgggtca
    15421 ttttctgcta aaatctgcgc cttgcgcttc tttagtgcac tgtctatggc taaccttttg
    15481 aataaattca ttatgacgag aatactcgct gcgataaccc ttttgctgag tatcgtattg
    15541 accattttgg tcactatttt ttgttccgtc ccgatcatca ttgccgggat tgtaaaacta
    15601 ttgctacctg tgccagtcat ttggcgaaag gtttcgcgtt tttgtgattt tatgatgtat
    15661 tgctggtgtg aaggtctggc ggtattactg catctgaacc cacacctgca atgggaagtt
    15721 cacgggctgg aagggttaag taagaagaac tggtatctgc ttatttgtaa ccaccgtagt
    15781 tgggcagata ttgtcgtact gtgcgtgctg tttcgtaagc acattccgat gaataaatat
    15841 ttcctcaagc aacagctggc ctgggtgcca tttcttggcc tggcgtgctg ggcgctggat
    15901 atgccgttta tgaagcgtta ttcccgcgct tatttattac gtcatccgga gcgacgtggt
    15961 caagatgttg aaaccactcg gcgttcttgt gaaaagtttc gcctgcatcc caccaccatt
    16021 gtcaatttcg ttgaaggctc ccgtttcacg caagaaaaac atcagcaaac ccactccact
    16081 tttcaaaact tgttgccacc aaaggcggca ggcattgcga tggcgctaaa tgtactgggt
    16141 aaacaattcg ataaactgct taatgttacg ctgtgttatc cggacaataa ccgtcagccg
    16201 ttcttcgata tgttaagcgg taaactgacg cggattgtcg tacatgtgga tttacagcct
    16261 attgccgatg agttacatgg cgactacatc aatgacaaaa gctttaagcg ccattttcag
    16321 caatggctga actcattgtg gcaggaaaaa gaccggcttc tgacgtctct catatcatca
    16381 cagcgtcaga ataagtaagt tttactcgcc tctgaatgag cagaggcgag tgagtatttt
    16441 aatgaacaaa acgtccggca cgagacataa attcttcttc gctcatctcc tgtccgttaa
    16501 aaacaacttt acccggtgta taacgcagct gcagcgaggc ggtattgttg tcaatcgtga
    16561 ttaactgaaa catctgacct aatgtcatca tcccgctgat ttgtttatca gcctgctttt
    16621 gcgctttttc cgcatccatt ccttcagata aattaagctg ttttgcggtt tctgtgacga
    16681 cattaagcgg taacttcaca tcaaaattga gcgatttgat atctttgttt gtggatgatg
    16741 aagatttggc tgggtcggca atactgatat ccagattggc atttagttcg ccgagtgcgt
    16801 tcttccatct taccggttgt ttaatggtcg gctcactttt ttgtaataac ggtaaatact
    16861 ctttgaacaa tgcagcattc acttcttgca gagcaacttc atcgtttgct aactcagggt
    16921 gtgcagcaag ctgcttctgc atcgcaatgt tatactggat gataaattgg cgcactgctg
    16981 agggatcaat agattcagca atcaatgacc actcaccact accgagttgc tgattccctt
    17041 ttgttaactt atcaagttcg taggtcagat tgacattgat ataatctttg tctttaacgc
    17101 gatccagcct ggtttttgca acgaaagcat cgatttgggc aacgtcttcc ccgtgattga
    17161 taatgttaat ctgattaaat ttcgactcgc tttcaccaac cggaaatttc ttttcttcta
    17221 atcgcgccag agaatcaatg gtaaaacttt ttgccgttac tttgttcatt ggtgataatt
    17281 gcagaacaat atcttcgact ttgccttcaa tatccaggtt agacaatgaa ctaccatcac
    17341 cattaaaagt aaactggccc tcaccaaaag aaaactttcc atactctgca ggggcaacat
    17401 ctaatgtcgt agagaatgag ccaccaaacg cgaatcgcaa tgtcgcttcc acgggcgatt
    17461 tatttttcgc ggcgataaat agcggttgtg tcagttcatt attcactacg ttcacttttg
    17521 ccgctgccag tgctgggatg acattaccat gcattaacat tgtgatgggc aacggaccgt
    17581 gctcaacatc cacatcaaat acaactttct ggcctggctt gatattgaga tcgggtgcac
    17641 cgttatcgaa tgtcatttgc atttgaatac gggaattaaa aaagcctttg tcaaagtttt
    17701 tataacttaa cgtcatatcg taggcatgtt cacctttctt tttcgcatcg ttaaaatctt
    17761 taataaactt ttcgatacca ggctgaattt gcgtacctgt gtaccatgtg ccaccacccc
    17821 agattacggc taacgcaaca ataacacctg tagctgactt acgtatcata taaatattcc
    17881 ctgataaaac agcaaaactt tgaatatcca cttatgctga aataatgtcc agcggcagga
    17941 tgcattatca gaatgtattt atatttatta aataataaaa aaagcccgtg aatattcacg
    18001 ggctttatgt aatttacatt gaattatttt ttctcggaca gatatttcac cgtatcagca
    18061 tactgctgaa caaaaacatc catattgctg gtatccatac cctgcggatt cagctgatat
    18121 ttaccgttaa caaacatcgc cggaacacca cgcaattgca catcagctgc ggctttttcc
    18181 tgctgagcga ccagagattt caccacgaag ctgttccacg ccgcgtcgta ctcttcacct
    18241 ttaatacctg cattgataaa tacatcgcgg atatcagatg ctgaacgaat ggtctgggtt
    18301 ttctgtacgc cttcaaacag cggaacagtc actttgtctt ccacgcccag cgccatcgcc
    18361 acagcccatg cctgagtcag atctttgccc aggtcaccac ccatgaagtt gacgtggtat
    18421 ttagtcatct tcacgccttc cggcagtttt ttcttcacat tatcagaaat atgcagaact
    18481 tcttcaaact gatagcagtg cgggcagaag aaagagaaaa actccagcac ttgcggcgcg
    18541 ccagctaccg gtttttccag ggtagtgtac tgtttaccat cttcatactg cgccgccgat
    18601 gcactaaacg ctaaaactaa accagccagc gccagccaaa tctttttcat gatctactct
    18661 ctccgattaa tacataggtg ttaattgcaa aggggattct tgtagaactt ttgcctgttc
    18721 tataaaagtc gccgtctgtc gtagccagta atcttccccg gttaaccacg ggaaattttt
    18781 cgggaacgcg ggatcagccc aatgccgcat tagccaggca agataataaa ccaaacgcat
    18841 ggcgcgtaaa ggttcaatca gtccgatttc agcggtgtcg aactcgctaa attcttcata
    18901 agcttcaata atagtttcca gttgcatccg ctgctgggct ttatcgccat tgagcaacat
    18961 ccacaaatcc tgaatagctg gaccattacg tgcatcatcc agatcaacaa acattggacc
    19021 atcgcgccag agaatattcc cggcgtggca gtctccatgt agccgcagaa cggtgaaatc
    19081 ttcccgccag tgtgctgtaa cggcggcgat cagctcatct gtcgctttca gaaatgccgc
    19141 tttcaacccg gaaggtatca gtgtagcgtc ctcaaacagc ttgcgaggct caatgagata
    19201 ttcattcaga ccgatggtcg ggcgatgaat aaaaagctgt ttgcgccccg tctggtgcat
    19261 acgccctaaa taacgcccaa ccgcttccat ctgatcgata ttatcagctt cgaactggcg
    19321 accaccgacg cttggaaaaa cagcgaaata aaatccctga tgattcaata aagtctgacc
    19381 gttaaaggcc acaggtgctg cgaccggaac ttcatcattt accagctgca acgcaaattg
    19441 atgttcttcg aggatttgat cggctgtcca acgttcaggg cgataaaatt tgacgacaaa
    19501 acgtcgacgc tcttcgtcct gaaattgata gacacggttt tcatagctgt taagcggggt
    19561 aagaccggaa tccacccgga tcccttgctc aaacagagcg tccatgatgg tatccgggtg
    19621 tagtgtctgg aaagtaaaag cgctgttgtt catcccatca tccggaaaac attacgaatg
    19681 atgaaggata tcatttcacg atgattttgg tggcgccgct tacaagcttt tactctttaa
    19741 ttacgccgcg agcgcgtaac agcgccgttt tgaagtcttc ttcataatct ttctgcaaac
    19801 ccgggatcac cgcatctttc gcggaatcac gcattttcag gtgatagatc agaatgtcat
    19861 ccgtcaaatc cgccagttcg ccgtcaaaac ctgactcttt cgccagtttc tgcaaaaatt
    19921 gcaacaagtt aaagtctggc tctttttgcc aggctggctg gaggagttca ataacttcat
    19981 tcagacgttt acatttcata gtaatgctcc ttactcttga gacagacacg ttagcagggt
    20041 caatcccaca ataaaagagg cgatatcggt gaatctgatg acgacgataa caggcgttgt
    20101 gctggcaggc ggtaaagcca gacgaatggg cggcgtagat aaaggattgc tggacttaaa
    20161 tggcaaaccg ctgtggcaac atgtcgctga cgcgcttaag acgcagctct ctcacgtcgt
    20221 ggttaatgct aatcgtcatc aggaaatcta tcaggcaggc ggtctgaaag tgattgaaga
    20281 ttcactggcg gattacccag gccctctggc aggaatgctt tcagtaatgc agcaggaagc
    20341 tggtgagtgg tttttgtttt gtccgtgcga tacgccttac attccccatg atttagccgc
    20401 ccggcttaat catcagcgca aagatgcgcc tgtcgtgtgg gtccatgacg gtgaacgcga
    20461 tcacccgact attgctctgg taaaccgcgc tattgagcct ttattactgg aatatctgca
    20521 agcaggagaa cgccgggtaa tggcatttat gcgtctggct ggcggtcatg cggttgattt
    20581 cagcgatcat aaagatgcat ttgttaacgt gaatacgcca gaggagcttg cccgatggca
    20641 ggaaaaacga tgataccgtt actcgccttt gccgcgtgga gtggtaccgg aaaaactacg
    20701 cttttgaaaa aactgatccc ggcattatgc gccagaggga tccgtccggg gctgattaag
    20761 catacgcacc atgatatgga tgttgataag ccaggcaaag atagctatga gctgcgcaag
    20821 gctggcgcgg cacaaaccat cgttgccagc cagcaacgat gggccttgat gacggaaaca
    20881 ccagacgaag aagagctgga tctacatttt ctcgcaagtc gaatggatac ctcaaagctg
    20941 gatttgattc tggtcgaagg gtttaagcat gaagagatcg caaagattgt gctgtttcgc
    21001 gatgcagccg gacatcgacc tgaagaatta gtgatagaca ggcatgttat tgctgtagcc
    21061 agtgatgtgc cgcttaatct tgatgtcgca ttactggata ttaatgatgt tgaggggctg
    21121 gctgattttg tggtggagtg gatgcaaaag cagaacggat aagcaagagt aggctggatc
    21181 agacgcagcc agtgtcgcat ccgacctttt tctgcaccag
//
LOCUS       DAJTYG010000065        21079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTYG010000065 DAJTYG010000000
VERSION     DAJTYG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.065"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            145..297
                     /gene="hokA"
                     /locus_tag="OO568_002587"
     CDS             145..297
                     /gene="hokA"
                     /locus_tag="OO568_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6641898.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(484..696)
                     /gene="cspA"
                     /locus_tag="OO568_002588"
     CDS             complement(484..696)
                     /gene="cspA"
                     /locus_tag="OO568_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6641899.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNYGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(977..1267)
                     /locus_tag="OO568_002589"
     CDS             complement(977..1267)
                     /locus_tag="OO568_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR6641900.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIERLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1701..2411
                     /locus_tag="OO568_002590"
     CDS             1701..2411
                     /locus_tag="OO568_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6641901.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="OO568_002591"
     CDS             complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="OO568_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6641902.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3539..4198)
                     /locus_tag="OO568_002592"
     CDS             complement(3539..4198)
                     /locus_tag="OO568_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6641903.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4351..6684
                     /gene="bisC"
                     /locus_tag="OO568_002593"
     CDS             4351..6684
                     /gene="bisC"
                     /locus_tag="OO568_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR6641904.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLAHCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6653..7093)
                     /locus_tag="OO568_002594"
     CDS             complement(6653..7093)
                     /locus_tag="OO568_002594"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6641905.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7090..7653)
                     /gene="tag"
                     /locus_tag="OO568_002595"
     CDS             complement(7090..7653)
                     /gene="tag"
                     /locus_tag="OO568_002595"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6641906.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKCGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7811..8509
                     /locus_tag="OO568_002596"
     CDS             7811..8509
                     /locus_tag="OO568_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6641907.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDSTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8738..9946
                     /locus_tag="OO568_002597"
     CDS             8738..9946
                     /locus_tag="OO568_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6641908.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10270..11961
                     /gene="eptB"
                     /locus_tag="OO568_002598"
     CDS             10270..11961
                     /gene="eptB"
                     /locus_tag="OO568_002598"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6641909.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12053..12129
                     /locus_tag="OO568_002599"
     tRNA            12053..12129
                     /locus_tag="OO568_002599"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12087..12089,aa:Pro,seq:cgg)
     gene            12873..14480
                     /gene="dppA"
                     /locus_tag="OO568_002600"
     CDS             12873..14480
                     /gene="dppA"
                     /locus_tag="OO568_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6641910.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14788..15807
                     /gene="dppB"
                     /locus_tag="OO568_002601"
     CDS             14788..15807
                     /gene="dppB"
                     /locus_tag="OO568_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6641911.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15817..16719
                     /gene="dppC"
                     /locus_tag="OO568_002602"
     CDS             15817..16719
                     /gene="dppC"
                     /locus_tag="OO568_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6641912.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16730..17713
                     /gene="dppD"
                     /locus_tag="OO568_002603"
     CDS             16730..17713
                     /gene="dppD"
                     /locus_tag="OO568_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6641913.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17710..18714
                     /gene="dppF"
                     /locus_tag="OO568_002604"
     CDS             17710..18714
                     /gene="dppF"
                     /locus_tag="OO568_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6641914.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18744..20015)
                     /locus_tag="OO568_002605"
     CDS             complement(18744..20015)
                     /locus_tag="OO568_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6641915.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20480..20587
                     /locus_tag="OO568_002606"
     CDS             20480..20587
                     /locus_tag="OO568_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6641916.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            20963..21070
                     /gene="ldrD"
                     /locus_tag="OO568_002607"
     CDS             20963..21070
                     /gene="ldrD"
                     /locus_tag="OO568_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCR6641917.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         5054 a         5208 c         5562 g         5255 t
ORIGIN      
        1 tccaattgaa tatcggaatc agacctatat gcctcgtgtt taactgtcca actttttggg
       61 gtcagtacaa agtgaggcta ccctatgcct gaacaccata aggtgtgcct cttactcgtt
      121 ggaaatcaac acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg
      181 atttgtttca cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat
      241 attaaacagg agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag
      301 tcttcggcgg gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccac
      361 atttattctt cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga
      421 aaaaaaataa aaaaatcccc gcaaaatggc agggatctta gattctgtgc ttttaatcag
      481 agattacagg ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa
      541 ggacactttc tgaccttcgt ccagagattt gtaaccatag ttctggatag cagagaagtg
      601 tacgaacaca tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt
      661 gaaccatttt acgataccag tcattttacc ggacatagtg tattaccttt aataattaag
      721 tgtgcctttc ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact
      781 acgaggggta tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc
      841 tgtacgtcaa accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt
      901 tttcatattt atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa
      961 taaagtggat aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca
     1021 aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta
     1081 ccatggcgac tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt
     1141 ttcgtaaccg ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg
     1201 tttcatcttt aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat
     1261 attccattga aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta
     1321 agaatagtcg ggaaaagcag acacagcggg gttaagtcag ggcatgctct ggctatgatt
     1381 cgattcagcc tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata
     1441 tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta
     1501 aatctctggc tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt
     1561 aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc
     1621 aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt
     1681 tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc
     1741 ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc
     1801 gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa
     1861 ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt
     1921 atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca
     1981 gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa
     2041 tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg
     2101 ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca
     2161 ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt
     2221 tcacgcaaca actggtgatg gttggagact acattgctca gcaaggtact caggtgagct
     2281 ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga
     2341 ttgcgccatt accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg
     2401 caacccaata aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc
     2461 ttagtccgcg acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat
     2521 caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagagccaat
     2581 atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc
     2641 ttgttcgaag acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag
     2701 tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat
     2761 tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag
     2821 gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg
     2881 ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa
     2941 gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt
     3001 atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt
     3061 tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt
     3121 atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt
     3181 aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt
     3241 cggcattttt tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc
     3301 ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg
     3361 aacggtgaaa tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat
     3421 aacggacggc ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata
     3481 tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt
     3541 acagcgggct taaggtaatt tctacacggc ggttttgtgc cttaccttct gcggtgctgt
     3601 tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc
     3661 cctgggtgat caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca
     3721 ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact
     3781 ctttcagcac cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggcgcgc
     3841 tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg
     3901 ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt
     3961 aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac
     4021 cgcgatcttt cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac
     4081 ctgcgccgat agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc
     4141 cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg
     4201 ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc
     4261 gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     4321 aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt
     4381 ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4441 tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4501 gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg
     4561 gaaaacccgc aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg
     4621 ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt
     4681 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     4741 caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt
     4801 gcggcacagg cgatcatgcc gtatgtcgta ggtggcagcg aagtttatca acagcaaacc
     4861 agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc
     4921 aatacgctga aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca
     4981 ctgcgtgaca gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat
     5041 ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg
     5101 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcat
     5161 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg
     5221 aaaaacgccg aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg
     5281 gcggctattt tccaccaaaa taccaccatg ctgatggcag gctggggaat gcagcgccaa
     5341 cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt ggggcaaatc
     5401 ggcacacccg gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg
     5461 cggcgttctg cggtgctctc ttccatgcag ggcagcttgc cgggtggctg cgatgcggtg
     5521 gataaaatcc ctgttgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa
     5581 cacaacggta tgaaccgaca tttcccggat attcgtttta tctggtgggc gggcggtgcc
     5641 aactttactc atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg
     5701 gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac acgcggatat cgttctgcct
     5761 gcgactacct cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat
     5821 ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt
     5881 tttgccgagt tgagtgaacg ttgggagaag ggcggctatg cgcggtttac ggaaggaaaa
     5941 agtcagctgc aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag
     6001 caggttgaat tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg
     6061 gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg
     6121 catccgttaa aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac
     6181 ggttacccgg attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     6241 gccgaaccag aacagttgca ggtactttct gcccatccgg cgcaccgcct gcacagccag
     6301 ctgaattaca gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt
     6361 catcctgacg atgctcaggc gcgcggcata accgaagggg atatggtgcg ggtgtggaac
     6421 tcccgtgggc agatccttgc tggagcggtc attagcgagg gaattaaacc tggcgtgatt
     6481 tgcattcacg aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac
     6541 ggcgcagtga acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6601 ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6661 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     6721 aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga tttttttgat
     6781 aaacctccag catcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa
     6841 taccgcgtct gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa
     6901 tgctgacaaa accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa
     6961 gataggcatc ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat
     7021 gcccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg
     7081 cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac
     7141 cagcccacat gcctgcataa aagagtaaca gattgtggtg ccgacaaact taaaaccaca
     7201 ttttttcagt gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa
     7261 cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcgggaaa
     7321 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     7381 tttccctcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7441 cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt
     7501 tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa
     7561 cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag
     7621 cgggccctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt
     7681 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     7741 actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg
     7801 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag
     7861 cgtggtaatc agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     7921 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga
     7981 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     8041 caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     8101 tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg atagcacaac
     8161 cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     8221 tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg
     8281 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     8341 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     8401 tatgttgctc aatcaaaata ttgccgccta tgctgcgcta actcaggcag aaaataccac
     8461 taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca
     8521 caattgaaac cattgaataa caacagttgt ggcgatagtg ggtgctaact taccaaataa
     8581 taaatttggt gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc
     8641 tgatggcatt tcatgccgtt tttccccaag cataaaatgc acttcgttat ggttgtcggc
     8701 agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg
     8761 tacccgctgg ctgacactca tcggcactat cattacccag tttgcgctgg ggtcggttta
     8821 tacctggagc ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt
     8881 cgctttctct ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa
     8941 attgcaggaa cgttttggcg ttaaacgcgt caccgtggct tccggcattt tgctgggatt
     9001 aggcttcttc ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt
     9061 gctggtcggt ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg
     9121 gttcccggag cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtctgggtag
     9181 cctgggtttc aaatttatcg acacgcagct gctcgaaacg gtgggtctgg aaaaaacctt
     9241 tgtgatttgg ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga
     9301 cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc
     9361 agagtcgatg cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat
     9421 gagcggcctg tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga
     9481 tgtggtttcc gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct
     9541 ggtgctgggc attctgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt
     9601 gatttcgctg gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt
     9661 tgcagcgatt gcctgcgtgg catttaactt tggcggcact attaccgtct ttccatcact
     9721 ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg
     9781 tttcggtatc ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt
     9841 gaccttctac gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacgattcg
     9901 tcagccagag cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct cagccgcgag
     9961 ctactgggct tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata aacaatgact
    10021 cgtaaaattt gggccagtta actggccctt tttactgctt tttgtaacgc taaagtctct
    10081 tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc acactttttc
    10141 gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac
    10201 ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt ttgtccaggg
    10261 tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt tttgcttgca
    10321 atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg cagctatgcg
    10381 cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc caccgtactg
    10441 gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg gcgtattctg
    10501 gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt ccttaatgtg
    10561 gtcattggtt atggcatcat cgcttccgtc atgaccaccg atatcgacct gtcaaaagaa
    10621 gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc tcttatcctt
    10681 atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc agggcagcgt
    10741 attcgcagcc tggtggtcgt cgtactggcg ggtattatgg tttgggcacc gattcgtttg
    10801 ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc gagttatggc
    10861 ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg gctgtatgcc
    10921 tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc gaagaagttt
    10981 acataccagg ctccacaaaa cgttgatgat acttatgtcg tgtttatcat cggtgaaacc
    11041 acgcgttggg accatatggg tattttcggc tatgagcgta ataccacgcc gaaactggcc
    11101 caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac caaactctca
    11161 ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg cacattaaaa
    11221 gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct ctacgctatg
    11281 cagagcgaaa tgtggttcta cagcaacacg atggcagaca acattgctta tcgtgagcag
    11341 attggtgcgg agccacgtaa tcgtggcaag ccggtagatg atatgttgct ggtagacgaa
    11401 atgcagcaat cgctagggcg caacccggat ggtaagcatc tgatcattct gcataccaaa
    11461 ggttcgcatt ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg gaagccggaa
    11521 tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta tgacaactcg
    11581 gtgacctatg tggatcactt tatctccagc gtgattgatc aggttcgcga taagaaagcg
    11641 attgtgttct acgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc
    11701 acgccgcgtg aactggcacc gccggagcag ttccgcgtac cgatgatggt ctggatgtca
    11761 gataaatatc tggaaaatcc ggccaatgcg caggcgtttg cgcagctgaa aaaagaagcc
    11821 gacatgaaag tgccacgccg tcacgtagag ctgtacgaca ccatcatggg ttgtcttggc
    11881 tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat cccgcagaca
    11941 aaagtggcag cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg
    12001 ctattaaggg attgacgagg gcgtatctgc gcagtaagat gcgctccgca ttcggtgatt
    12061 ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat
    12121 caccgaccaa attcgaaaag cctgctcagc gagcaggttt ttttgcattt gtagctcatg
    12181 aggatgagaa cctccggggg cagggaggct cgactcgacg taggcctgat aagacgcgcc
    12241 agcgtcgcat caggcaacgg caccgcactt attgtcggat gcggcgcgac cgccttattc
    12301 gaccaacaca ccacacattt tcctgccaac tttgcgcccc atatctcact atccattaac
    12361 ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt ttgcgttact
    12421 tatggctggc gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag
    12481 atatccacct tttcctcaga tttttttcat taaatgcata aaacttaatc accaaatttt
    12541 gctaaatatt aagctgattg ttctggttat gttggagtag cacaaatttc tctgctgcaa
    12601 atagctgttt gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc
    12661 ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt
    12721 tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg
    12781 ttgacccgac cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt
    12841 aaaacaacaa acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagtcaggga
    12901 tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa
    12961 ctctggttta ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg
    13021 gcaccaccta tgacgcctct tccgtcccgc tttataaccg cttggttgaa tttaaaatcg
    13081 gcaccaccga agtgatcccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa
    13141 cctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga
    13201 cgcgcgaaat gaacgccgat gacgtggtgt tctcgttcga tcgtcagaaa aacgcgcaaa
    13261 acccgtacca taaagtttct ggcggcagct acgaatactt cgaaggtatg ggcttgccgg
    13321 agctgatcag cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg ctgactcgac
    13381 cggaagcgcc gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat
    13441 atgctgacgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggaa
    13501 ccggtccgtt ccagttacag cagtaccaaa aagattcccg tatccgctac aaagcgtttg
    13561 atggctactg gggcaccaaa ccgcagatcg atacgctggt cttctctatt acccctgatg
    13621 cttccgtgcg ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg tacccgaacc
    13681 cggcagacat cgctcgcatg aagcaggata aatccatcaa cctgatggaa atgccggggc
    13741 tgaacgtcgg ctacctctcg tataacgtgc agaaaaaacc gctggatgac gtgaaagttc
    13801 gccaggcact gacctacgcg gtgaacaaag acgcgatcat caaagcggtt tatcagggcg
    13861 cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc
    13921 aggattacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg ggtctggaaa
    13981 aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac ccgaacgctc
    14041 gccgcatggc ggagatgatt caggcagact gggcaaaagt gggcgtgcag gccaaaatcg
    14101 tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgaacat cagacggtaa
    14161 tgatgggctg gactggcgat aacggggacc cggataactt cttcgccacc ctgttcagct
    14221 gcgccgcctc tgaacaaggt tctaactact caaaatggtg ctacaaaccg tttgaagatc
    14281 tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg tacaagcaag
    14341 cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg
    14401 aaccggtacg caaagaagtc aaaggctatg tggttgatcc attaggcaaa catcacttcg
    14461 aaaacgtctc tatcgaataa ttaaaagcca tacaagactg atggcaaagg caaaactgcc
    14521 tgatgtgctc cgcttatcag gcctacgaaa attctgcaat gtattgaatt tgcacgattt
    14581 tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caaagcgcac tttgtcaaca
    14641 atctgtatac ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag caatacttca
    14701 ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat acagacacgc agttccaggc
    14761 tgcgggtcac tatagagaat ccgggttatg ttgcagttta ttctccgacg tttgggactc
    14821 gtcatcccca cgtttatcgg tattaccctt ctcacatttg cctttgtcca catgatcccg
    14881 ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg tcacgtgcag
    14941 ctgctggctg aactcggctt agataaaccg atgtggcagc agtatctcca ttacatttgg
    15001 ggcgtaatgc acggcgatct aggcatttca atgaaaagcc gaattccagt atgggaagag
    15061 ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct gcgcgatgat ttttgctacg
    15121 gcagtcggta ttccggttgg tgtgctggct gcggttaaac gcggttccat tttcgatcac
    15181 actgcagttg gcctggcgct gaccggctac tcgatgccga tcttctggtg gggcatgatg
    15241 ctgatcatgc tggtttcggt gcactggaac ctgacgcccg tctccggtcg cgtgagcgat
    15301 atggtgttcc tcgatgactc caatccgtta accggtttta tgctaatcga caccgccatc
    15361 tggggtgaag acggcaactt tatcgatgcc gtcgcccata tgatcttgcc cgccattgtg
    15421 ctgggtacta ttccgctggc ggtcattgtg cgtatgacac gctcctcgat gctggaagtg
    15481 ctgggcgagg attacatccg caccgcgcgc gccaaagggc taacccgcat gcgggtgatt
    15541 atcgtccatg cgctgcgtaa cgcgatgctg ccggtagtga ccgttatcgg cctccaggtg
    15601 ggaacattgc tggcgggggc gattctgacc gaaaccatct tctcgtggcc cggtctgggg
    15661 cgctggttga ttgacgcact gcaacgccgc gactatccgg tagtgcaggg cggcgtattg
    15721 ctggtggcga cgatgattat cctcgtcaac ttgctggtcg atctgctgta cggcgtggtg
    15781 aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt cacaggttac tgaaaataaa
    15841 gtgattagcg caccggtgcc gatgaccccg ttacaggagt tctggcacta ttttaaacgc
    15901 aacaaaggcg cggtcgtcgg gctggtttac gtcgccatcg tgctgttcat cgcgatcttt
    15961 gccaactgga ttgcgcctta taacccggcg gatcagttcc gcgatgcgct gctcgccccg
    16021 ccagcctggc aggaaggcgg cagtatggca cacttgctgg gcaccgatga cgtcggtcgt
    16081 gatgtgctgt cgcggctgat gtacggcgcg cgtttatcgc tgctggttgg ctgtctggtg
    16141 gtggtgttgt cgctgattat gggcgttatt ctcggcctga tcgccggtta cttcggcggc
    16201 ctggttgata acattatcat gcgcgtggtc gatatcatgc tggcgctgcc aagtctgctg
    16261 ttggcactgg tgctggtggc gattttcggc ccgtcgattg gtaacgccgc gctggcgctg
    16321 accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct ggtggaggtg
    16381 aatcgcgatt acgtcaccgc ctctcgcgtg gcaggtgccg gggcgatgcg ccagatgttt
    16441 attaacatct tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct cggtttctct
    16501 aacgccattc tcgatatggc tgctcttggt ttcctcggca tgggggcaca gccgccaacg
    16561 cctgagtggg gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc ctggtgggtc
    16621 gtgaccttcc ctggtctggc gatcctgttg acggtgctgg catttaacct gatgggtgac
    16681 ggtctgcgtg acgcgctcga tcccaaactg aagcagtaag aggttcgaga tggcgttatt
    16741 aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc gcgccgtaga
    16801 ccgcatcagc tacagcgtaa aacagggcga agtggtcggg attgtgggtg agtccggctc
    16861 cggtaagtcg gtcagttcac tggcgattat ggggctgatt gattatccgg gccgcgtaat
    16921 ggcagaaaaa ctggagttta acggccagga tttgcagcgt atctcagaaa aagagcgccg
    16981 caacctggtg ggtgccgaag tggcgatgat cttccaggac ccgatgacca gccttaaccc
    17041 atgctacacc gtgggtttcc agattatgga agcgattaaa gtgcatcagg gcggcaacaa
    17101 aagtacccgc cgtcagcggg cgattgacct gctgaatcag gtcggtattc ccgatccggc
    17161 ttcgcgtctg gatgtttacc cgcatcagct ttccggcggc atgagccagc gcgtgatgat
    17221 cgccatggcg attgcctgtc ggccaaaact gctgattgcc gatgaaccga ccaccgcgct
    17281 ggacgtgacc attcaggcgc aaatcatcga actactgctg gagctacagc agaaagagaa
    17341 catggcgctg gtgttaatta cccatgacct ggcgctggtg gcggaagctg cgcataaaat
    17401 cattgtgatg tatgcaggtc aggtagtaga aacgggcgat gcacacgcca tcttccatgc
    17461 gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg ccagaatttg ctcaggacaa
    17521 agaacgtctg gcgtcgttgc caggtgttgt tcccggcaag tatgaccgcc cgaacggctg
    17581 cctgcttaac ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag aaccggcgct
    17641 gaatatgctc gctgatgggc gtcagtccaa atgccattac ccacttgatg atgccgggag
    17701 gcccacacta tgagtacgca agaggccacc tcgcaacaac cgctgttgca ggctatcgac
    17761 ctgaaaaaac attatccggt gaagaaaggc atgttcgtgc cggaacgtct ggtgaaggcg
    17821 ctggacggcg tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt gggcgaatct
    17881 ggctgcggta aatcgaccct cggtcggttg ctgacgatga tcgaaacgcc caccggcggc
    17941 gagctgtatt atcaggggca ggatctgctc aagcacgatc cgcaggcgca gaagctgcgt
    18001 cggcagaaaa tccagatcgt cttccagaat ccctacggtt cgctgaatcc gcgtaagaaa
    18061 gttgggcaaa ttctcgaaga gccgctgctt attaacacca gcttaagcaa agaacagcgt
    18121 cgggaaaaag ccctgtcgat gatggcaaaa gtcggcctga aaaccgagca ctatgaccgc
    18181 tatccgcaca tgttttccgg cggacaacgt cagcgtatcg ctattgcccg tggtctgatg
    18241 ctcgacccgg atgtggtgat tgccgatgag ccggtttctg cgctggacgt ctcagtgcgt
    18301 gcgcaggtgc tgaatctgat gatggatttg cagcaggagt tggggctttc ttatgtcttt
    18361 atctcccacg acctgtcggt tgtggagcac attgctgatg aagtgatggt gatgtaccta
    18421 ggccgctgcg tggagaaagg aacgaaggac caaatcttca ataacccgcg ccatccgtac
    18481 acccaggcgc tgctctccgc gacgccacgc ctgaacccgg acgatcgtcg tgaacgcatc
    18541 aaactcaccg gtgaactgcc aagcccgctg aatcctccgc cgggttgcgc cttcaacgcc
    18601 cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa agactacgga
    18661 ggtcaactgg tagcttgttt tgctgttgat caggatgaaa atccgcagcg ttaactcaca
    18721 aaaccggggc catgcgctcc ggtttactca atcagcttca acagtggcga caagcatagc
    18781 aaaatgccgt aaagcagaat cagccaggct ttaaatccgc gaagtttttc cagttgtgcg
    18841 actttataga tcaggaaaaa cggaatgagg cacgacacaa tgccatataa cgggcttccc
    18901 aactgaaaga acaccagcac tgatacacga aacgaaaccc aaatcgtcaa cgtaatgacg
    18961 ataaaagcgc agatcgccag agtcagcatg cgtgagttaa ttttcttggt atcaataatt
    19021 cggcttaaca gattgagaat aatgccttta atagcctcgt ggaaaccgag ataaatgcca
    19081 aagaatgcgg tcagtacggc aaagatattc agcaccgtag aggtgatatg aatgatatgt
    19141 ccagggatca cctgcgcggc cagtgccagt gccgagatat tttgttcaaa ggcagaaacg
    19201 gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat caccgcgatg
    19261 agcgtgatat aactaattcg gtgggtacgc agcgcgagcc gggttgccag tactttatct
    19321 gcttcccgtt tacgataggc aatattcatt gggttaagta cctgaataaa taccgcagaa
    19381 aagaagcaaa atggaatggt aagcaggaca tcgcggaaaa agacggaggc ttgcgggaag
    19441 gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac gacaataatc
    19501 cctactttga ccaccaccat tggcccagaa atcttaaaca gtaatcgttc accaccagac
    19561 gcaatcgcta tcagtacggc gaaaatagcg actttataaa gtagagattg tgaaagatcg
    19621 gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac ggagagagag
    19681 tagataaaaa tcccgtggat aatcatcaaa aagtagataa ccccgaggaa aattccccag
    19741 ttcttcccca ggtaatgact gataatatcg gtgtagtcat tacaggaatc gctttcagaa
    19801 agggttttta aataaatgtc ctgcactacc caggtggcag gataagcaat gatcgctgcg
    19861 gtaataaata cccaaattcc cttcaagcca atttgtactg gcatcagcac ggttccggca
    19921 ccaatcgcca tgcctatgca taataaaacc cagccgaagt cgtagcgtgt aaacggcaat
    19981 ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga aagaagtcgg
    20041 cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca ggtggagaag tcgaatacgc
    20101 agtatgtaaa aaatgaggca acagattcac tggggaggtc gcgaggaggc gcaattctac
    20161 aaaattgcgc atttcactac cagtctggag tcagcccgga tatgaactgc aactattgca
    20221 aataaagaaa ttaagctgga aggcatcgcg caatgaggaa gattgccgga aaaagcaatc
    20281 gtaaaatcag cggcaagccg gaagggttct ggtgaggcgc aatgttgcgg gggctttatc
    20341 cctggtggca ttggttgctg gagagagaaa acccccgcac gttgcaggta tgtacctgac
    20401 aacataacgg gggctaatct tgaacctaga ccactcaaga atagccgcga aacgttgtca
    20461 ttacaacaca ggcggctata tgacgctcgc agagctgggc atggccttct ggcatgattt
    20521 agcggctccg gtcattgctg gcattcttgc cagtatgatc gtgaactggc tgaacaagcg
    20581 gaagtagcgt gtcatgtggg cgtcaggctg ccgtaatggc aatttgcgcc cggaccaggc
    20641 cgcaggggtg aaactctgcg gcctttttcg taagaaaaat gcaacggttg caagcggata
    20701 atattctgcg gaatgcgcag cacagagtga agatgagacg gattccggaa cagaaaaaca
    20761 gtcgtagaat cagcgtcaag ccggaaggat tccggtgagg cgcaatgttg cgggggctta
    20821 atccctggtg gcattggttg ctggagagag aaaacccccg cacgttgcag gtatgcacct
    20881 gacaacacca cgggggccat tcttgcatcc aaacaataca agaatagccg cgaaacgttg
    20941 tcattacaat acaggcggct atatgacgtt cgcagaactg ggcatggcct tctggcatga
    21001 tttagcggct ccggtcattg ctggtattct tgccagtatg atcgtgaact ggctgaacaa
    21061 gcggaagtaa cgtgccatg
//
LOCUS       DAJTYG010000066        20677 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTYG010000066 DAJTYG010000000
VERSION     DAJTYG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.066"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            117..881
                     /gene="ycaL"
                     /locus_tag="OO568_002608"
     CDS             117..881
                     /gene="ycaL"
                     /locus_tag="OO568_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6641918.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            1054..1737
                     /gene="cmk"
                     /locus_tag="OO568_002609"
     CDS             1054..1737
                     /gene="cmk"
                     /locus_tag="OO568_002609"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6641919.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            1848..3521
                     /gene="rpsA"
                     /locus_tag="OO568_002610"
     CDS             1848..3521
                     /gene="rpsA"
                     /locus_tag="OO568_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6641920.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            3681..3965
                     /gene="ihfB"
                     /locus_tag="OO568_002611"
     CDS             3681..3965
                     /gene="ihfB"
                     /locus_tag="OO568_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6641921.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            4172..6391
                     /locus_tag="OO568_002612"
                     /pseudo
     CDS             4172..6391
                     /locus_tag="OO568_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            6428..8176
                     /gene="msbA"
                     /locus_tag="OO568_002613"
     CDS             6428..8176
                     /gene="msbA"
                     /locus_tag="OO568_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6641922.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            8173..9159
                     /gene="lpxK"
                     /locus_tag="OO568_002614"
     CDS             8173..9159
                     /gene="lpxK"
                     /locus_tag="OO568_002614"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6641923.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVVMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            9196..10428
                     /locus_tag="OO568_002615"
     CDS             9196..10428
                     /locus_tag="OO568_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6641924.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            10480..10662
                     /gene="ycaR"
                     /locus_tag="OO568_002616"
     CDS             10480..10662
                     /gene="ycaR"
                     /locus_tag="OO568_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6641925.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            10659..11405
                     /gene="kdsB"
                     /locus_tag="OO568_002617"
     CDS             10659..11405
                     /gene="kdsB"
                     /locus_tag="OO568_002617"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6641926.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLVEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            11559..12452
                     /locus_tag="OO568_002618"
     CDS             11559..12452
                     /locus_tag="OO568_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6641927.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12429..13208)
                     /gene="elyC"
                     /locus_tag="OO568_002619"
     CDS             complement(12429..13208)
                     /gene="elyC"
                     /locus_tag="OO568_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6641928.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGTRVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            13344..14129
                     /gene="cmoM"
                     /locus_tag="OO568_002620"
     CDS             13344..14129
                     /gene="cmoM"
                     /locus_tag="OO568_002620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6641929.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGRVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            14126..15448
                     /gene="mukF"
                     /locus_tag="OO568_002621"
     CDS             14126..15448
                     /gene="mukF"
                     /locus_tag="OO568_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6641930.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            15429..16133
                     /gene="mukE"
                     /locus_tag="OO568_002622"
     CDS             15429..16133
                     /gene="mukE"
                     /locus_tag="OO568_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6641931.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            16133..20593
                     /gene="mukB"
                     /locus_tag="OO568_002623"
     CDS             16133..20593
                     /gene="mukB"
                     /locus_tag="OO568_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6641932.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMLRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQSEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGRDEAPSQAS"
BASE COUNT         4972 a         5019 c         5716 g         4970 t
ORIGIN      
        1 attgtcggcg ttttttttcg gaccttgtga gtcattttga ttaatggtag cgtcgcttgt
       61 caatgtaagt tgttgataca taatatttat atatgattaa tcaacggatg attcacatga
      121 agaatactaa attactgctg gcgattgcga cctctgcagc attactgaca gggtgtcaaa
      181 atacccacgg tattgatacc aatatggcta tcagctccgg tttaaatgcc tataaagcag
      241 caacattaag cgatgccgat gcaaaagcga ttgccaatca gggctgtgcc gaaatggaca
      301 gcggcaatca agtcgcaagt aaatccagca agtacggtaa acgtctggca aaaatcgcca
      361 aagcattggg taacaatatt aatggcacgc cggtcaacta taaggtttat atgaccagcg
      421 acgtcaacgc atgggcgatg gcgaacggct gtgttcgtgt ctacagtggc ctgatggaca
      481 tgatgaatga caacgaaatt gaaggtgttc tgggccatga actgggccac gtcgcgttgg
      541 gtcactcgct ggctgaaatg aaagcttctt atgcgatcgt tgccgcacgc gatgccattt
      601 cagctaccag cggtgtggct tcccagcttt cccgctcaca attgggtgat atcgcagaag
      661 gcgctattaa tgctaaatac tcccgtgata aagagtccga agcagatgat ttctcttttg
      721 atctgttgaa gaaacgtggc atcagcaccc aggggctggt tggcagcttt gaaaaactgg
      781 ctagcctgga tggcggtcgc acccagtcca tgtttgactc tcacccacca tcaacagagc
      841 gtgcgcaaca catccgtgat cgtatcgcct ctggtaagta aatcattgtc atctttcggg
      901 ctggtcttct gccagcccgc tataattgcg caataaatcc ccatctgaat acagacaaaa
      961 ctggtttttg cacacaacgt taacgatttg tggcgtcggc gcgtataatg cgcgcggtta
     1021 tgttaacggt acgcctgttt taaggagata aagatgacgg caattgcccc ggttattacc
     1081 attgatggcc caagcggtgc agggaaaggc accttgtgta aggctatggc ggaagcgttg
     1141 caatggcatc tgctggactc gggtgcaatt tatcgcgtac tggcattggc ggcattacat
     1201 caccatgttg atgttgcgtc ggaagatgcg ctggtaccgc tggcatccca tctggatgta
     1261 cgttttgtgt cgaccaatgg caatctggaa gtgatcctcg aaggggaaga tgtcagcggc
     1321 gaaattcgta ctcaggaagt ggcgaatgcg gcttcacaag tcgcggcatt cccacgcgtt
     1381 cgtgaagcat tattgcgtcg ccaacgcgcg tttcgcgaat taccaggtct gattgccgat
     1441 ggccgcgaca tgggaacggt ggtattccct gatgcaccag tgaaaatttt ccttgacgcc
     1501 tcctcggaag aacgtgcgca tcgccgcatg ctacagttgc aggagaaggg ctttagtgtt
     1561 aacttcgagc gccttttggc cgagatcaaa gaacgcgacg accgcgatcg taaccgagcg
     1621 gtagcgccac tggttccggc agccgatgct ttagtgttgg attccaccac cttaagcatt
     1681 gagcaagtga ttgaaaaagc gctacaatac gcgcgccaga aattggctct cgcataagcg
     1741 accgaatttg cagtaccccc gttgcaatgg aatgacagcg ggtatgttaa acaaccccat
     1801 ccggcatgga gccaggtgga cgttaaatat aaacctgaag attaaacatg actgaatctt
     1861 ttgctcaact ctttgaagag tccttaaaag aaatcgaaac ccgcccgggt tctatcgttc
     1921 gtggcgttgt tgttgctatc gacaaagacg tagtactggt tgacgctggt ctgaaatctg
     1981 agtccgccat cccggctgag cagttcaaaa acgcccaggg cgagctggaa atccaggtag
     2041 gtgacgaagt tgacgttgct ctggacgcag tagaagacgg cttcggtgaa actctgctgt
     2101 cccgtgagaa agctaaacgt cacgaagcct ggatcacgct ggaaaaagct tacgaagatg
     2161 ctgaaactgt taccggtgtt atcaacggca aagttaaggg cggcttcact gttgagctga
     2221 acggtattcg tgcgttcctg ccaggttctc tggtagacgt tcgtccggtg cgtgacactc
     2281 tgcacctgga aggcaaagag cttgaattta aagtaatcaa gctggatcag aagcgcaaca
     2341 acgttgttgt ttctcgtcgt gccgttatcg aatccgaaaa cagcgcagag cgcgatcagc
     2401 tgctggaaaa cctgcaggaa ggcatggaag ttaaaggtat cgttaagaac ctcactgact
     2461 acggtgcatt cgttgatctg ggcggcgttg acggcctgct gcacatcact gacatggcct
     2521 ggaaacgcgt taagcatccg agcgaaatcg tcaacgtggg cgacgaaatc actgttaaag
     2581 tgctgaagtt cgaccgcgaa cgtacccgtg tatccctggg cctgaaacag ctgggcgaag
     2641 atccgtgggt agctatcgct aaacgttatc cggaaggtac caaactgact ggtcgcgtga
     2701 ccaacctgac tgactacggc tgcttcgttg aaatcgaaga aggcgttgaa ggtctggtac
     2761 acgtttccga aatggattgg accaacaaaa acatccaccc gtccaaagtt gttaacgttg
     2821 gcgatgtagt ggaagttatg gttctggata tcgacgaaga acgtcgtcgt atctccctag
     2881 ggctgaaaca gtgcaaagct aacccgtggc agcagttcgc ggaaacccac aacaagggcg
     2941 accgtgttga aggtaaaatc aagtctatca ctgacttcgg tatcttcatc ggcctggacg
     3001 gcggcatcga cggcctggtt cacctgtctg acatctcctg gaacgttgca ggcgaagaag
     3061 cagttcgtga atacaaaaaa ggcgacgaaa tcgctgcagt tgttctgcag gttgacgcag
     3121 aacgtgaacg tatctccctg ggcgttaaac agctcgcaga agatccgttc aacaactggg
     3181 ttgctctgaa caagaaaggc gctatcgtaa ccggtaaagt aactgcagtt gacgctaaag
     3241 gcgcaaccgt agaactggct gacggcgttg aaggttacct gcgtgcttct gaagcatccc
     3301 gtgaccgcgt tgaagacgct accctggttc tgagcgttgg cgacgaagtt gaagctaaat
     3361 tcaccggcgt tgatcgtaaa aaccgcgcaa tcagcctgtc tgttcgtgcg aaagacgaag
     3421 ctgacgagaa agatgcaatc gcaactgtta acaaacagga agatgcaaac ttctccaaca
     3481 acgcaatggc tgaagctttc aaagcagcta aaggcgagta attctctgac tcttcgggat
     3541 ttttattccg aagtttgttg agtttacttg acagattgca ggtttcgtcc tgtaatcaag
     3601 cactaagggc ggctacggcc gcccttaatc aatgcagcaa cagcagccgc ttaatttgcc
     3661 tttaaggaac cggaggaatc atgaccaagt cagaattgat agaaagactt gccacccagc
     3721 aatcgcacat tcccgccaag acggttgaag atgcagtaaa agagatgctg gagcatatgg
     3781 cctcgactct tgcgcagggc gagcgtattg aaatccgcgg tttcggcagt ttctctttgc
     3841 actaccgcgc accacgtacc ggacgtaatc cgaagactgg cgataaagta gaactggaag
     3901 gaaaatacgt tcctcacttt aaacctggta aagaactgcg cgatcgcgcc aatatttacg
     3961 gttaagtttt tactcaaact tgaacgcgag aaaagcacct gtcgggtgct tttttcaatt
     4021 ctctaatcag gaactggaag ctgcctcgca gagttttgag cagttttcac cctttcgtta
     4081 aattcttctg aatgtgcctc ggggaacgca aaattcccac cagacaaccg ctcaacaaag
     4141 ttgcacactt tccataaaca gggaggggtg catgaaaata acgacagtcg gcgtatgcat
     4201 aatttgcgga atttttccgt tgctgatttt gccccaattg cctaggacat taacccttgc
     4261 gtttctgact ctcttcgcct gcgtactggc atttatccct gttaaaaccg tccgttatat
     4321 cgcgctgacg ttgctgtttt tcgtttgggg catattatca gcaaagcaaa ttttgtgggc
     4381 aggagaaacc ttaactggcg cgacgcagga tgcaattgtt gagatcactg caactgacgg
     4441 catgaccact cattacggtc aaattactta tctacaaggt cgacgtatat tccctgcgcc
     4501 aggccttgtg ctgtatggcg aataacttcc gcaagcggtt tgtgctggac aacaatggtc
     4561 aatgaaactc aaagttcgtg cagttcatgg ccaacttaat gatggcggct ttgatagcca
     4621 gcgttatgcc attgcccagc atcagccgct caccggccgt tttctgcagg caagtgtcat
     4681 tgaaccgaat tgtagcctgc gtgcacagta tctggcgtca ctacaaacaa cgctgcaacc
     4741 ctatccgtgg aatgcggtta ttcttggttt aggtatgggg gaacggttat ccgtccccaa
     4801 agaaatcaaa aatatcatgc gcgatactgg aacggcgcat ttaatggcga tatcgggatt
     4861 gcacatcgct tttgcggcgt tgctggctgc cggactcatt cgcagtggac aaatttttct
     4921 gcctgggcgc tggatccact ggcaaatacc attaattggc ggaatctgct gtgctgcttt
     4981 ttatgcctgg ttgacgggaa tgcaacctcc tgcattgcgt accgtggtgg cgcttgctac
     5041 gtggggaatg cttaagttaa gtgggcgaca gtggagtggc tgggatgtat ggatatgttg
     5101 tctggcggca attttgctga tggatcctgt tgccattctc tcgcaaagtt tatggctctc
     5161 tgccgctgcg gtcgcggcac tgattttttg gtatcagtgg tttccctgtc ctgagtggca
     5221 actgccgccg gtattgcgtg cagttgtttc cctcatccat ctgcaactgg gaatcacact
     5281 cctgcttatg cccatgcaaa tcgtcatatt tcatggcatt agtctgacct cgtttattgc
     5341 aaatctattt gcaattccct tggtgacatt tatcacggtt ccgttgatcc tcgccgcgat
     5401 ggttgtgcat tttagcgggc cgttaatcct ggaacagggg ttaaagagtt tgccggaagg
     5461 gtggatcaac attgttgaat gttggcaatg gctatcattt tccccatggt tcttactggt
     5521 ggtatggtga ttaaacgcct ggcgaacgtt gccagtaatg tgtgtggctg gaggcttgct
     5581 gatgtgctgg ccgctgtggc aaaaacctcg acctgacgag tggcagctgt acatgcttga
     5641 tgtcgggcaa gggctggcaa tggtgatagc cagaaacggc aaagcgattc tctatgacac
     5701 aggactggcc tggcctgaag gggatagtgg gcaacaactg attatcccct ggctccactg
     5761 gcataatctt gaaccggaag gcgttattct gagccatgaa catctggatc accggggagg
     5821 gctggattca atattgcata tatagccgat gttatggatc agaagtccgt taaactggga
     5881 acatcatcag ccctgtgtgc gtggcgaagc gtggcagtgg caaggattgc gtttcagcgc
     5941 gcactggcct ttacaaggta gcaacgataa gggaaataac cattcctgtg tggttaaggt
     6001 tgatgacggg acgaatagca ttcttctaac cggtgatatt gaagccccag ctgaacaaaa
     6061 gatgctaagc cgttactggc agcaagtgca ggcaacattg cttcaggtac ctcaccatgg
     6121 cagtaatacc tcatcatcgt tgccattaat tcagcgagtg aatggaaaag tggcactcgc
     6181 atcggcatcg cgctataacg catggcgatt gccctctaac aaagttaagc atcgctatca
     6241 acagaaagga tatcaatggc ttgatactcc acatcagggt caagtgacgg tcaatttttc
     6301 agcacaaggc tggcggatta gcagcctcag agagcaaatt ttacctcgtt ggtatcatca
     6361 gtggtttggc gtgccagtgg ataacgggta gaatatgcgg ctatttcaac aaatgctggt
     6421 tttttgaatg cataacgaca aagatctctc tacgtggcag acattccgcc gactgtggcc
     6481 aaccattgcg cctttcaaag cgggtctgat cgtggcgggc gtagcgttaa tcctcaacgc
     6541 agccagcgat accttcatgt tatcgctcct taagccactt cttgatgatg gctttggtaa
     6601 aacagatcgc tccgtgctgg tgtggatgcc gctggtggtg atcggactga tgattttacg
     6661 tggtatcacc agctatgtct ccagctactg tatctcctgg gtatcaggaa aggtggtaat
     6721 gaccatgcgt cgccgcctgt ttggtcacat gatgggaatg ccagtttcat tctttgacaa
     6781 acagtcaacg ggtacgctgt tgtcacgtat cacctacgat tccgaacagg ttgcttcttc
     6841 ttcttccggc gcactgatta ctgttgtgcg tgaaggtgcg tcgatcatcg gcctgttcat
     6901 catgatgttc tattacagtt ggcaactgtc gatcattttg attgtgctgg caccgattgt
     6961 ttcgattgcg attcgcgtag tatcgaagcg ttttcgcaac atcagtaaaa acatgcagaa
     7021 caccatgggg caagtgacca ccagtgcaga acaaatgctg aagggccaca aagaagtatt
     7081 gattttcggt ggtcaggaag tggaaacgaa acgcttcgat aaagtcagta accgaatgcg
     7141 tcttcagggg atgaaaatgg tttcagcctc ttccatctct gatccgatca ttcagctgat
     7201 cgcctctttg gcgctggcgt ttgttctgta tgcggcgagc ttcccaagtg ttatggataa
     7261 cctgactgcc ggtacgatta ccgttgtttt ctcttcaatg attgcactga tgcgtccgct
     7321 gaaatcgctg accaacgtta acgcccagtt ccagcgcggt atggcggctt gtcagacgct
     7381 gtttaccatt ctggacagtg agcaggagaa agacgaaggt aagcgcgtga tcgagcgtgc
     7441 gactggcgac gtggaattcc gcaatgtcac ctttacttat ccgggacgtg acgtacccgc
     7501 attgcgtaac atcaacctga aaattccggc agggaagacg gttgctctgg ttggacgctc
     7561 tggttcaggt aaatcaacca ttgccagcct gatcacgcgt ttttacgata ttgatgaagg
     7621 cgaaatcctg atggatggtc acgatctgcg cgagtatacc ctggcgtcgt tacgtaacca
     7681 ggttgctctg gtgtcgcaga atgtccatct gtttaacgat acggttgcta acaacattgc
     7741 ttacgcacgg actgaacagt acagccgtga gcaaattgaa gaagcggcgc gtatggccta
     7801 cgccatggac ttcatcaata agatggataa cggtctcgat acagtgattg gtgaaaacgg
     7861 cgtgctgctc tctggcggtc agcgtcagcg tattgctatc gctcgagcct tgctgcgtga
     7921 tagcccgatt ctgattctgg atgaagctac ctcggctctg gataccgaat ccgaacgtgc
     7981 gattcaggcg gcactggatg agttgcagaa aaaccgtacc tctctggtga ttgcccaccg
     8041 cttgtctacc attgaaaagg cagacgaaat cgtggtcgtc gaggatggtg tcattgtgga
     8101 acgcggtacg cataacgatt tgcttgagca ccgcggcgtt tacgcgcaac ttcacaaaat
     8161 gcagtttggc caatgatcga aaaaatctgg tctggtgaat cccctttgtg gcggctattg
     8221 ctgccactct cctggttgta tggtctggtg agtggcgcga tccgtctttg ctataaacta
     8281 aaactgaagc gcgcctggcg tgcccccgta ccggttgtcg tggttggtaa tctcaccgca
     8341 ggcggcaacg gaaaaacccc ggtcgttgtc tggctggtgg aacagttgca acagcgcggt
     8401 attcgcgtgg gggtcgtatc gcggggatat ggtggtaagg ctgaatctta tccgctgtta
     8461 ttgtcggcag ataccaccac agcacaggcg ggtgatgaac ctgtgttgat ttatcaacgc
     8521 actgatgcgc ctgttgcggt ttctcccgtt cgttctgatg cggtaaaagc cattctggcg
     8581 caacaccctg atgtgcagat catcgtaacc gacgacggtt tacagcatta ccgtctggcg
     8641 cgtgatgtgg aaattgtcgt tattgatggt gtgcgtcgct ttggcaatgg ctggtggttg
     8701 ccggcggggc caatgcgtga gcgagcgggg cgcttaaagt cggttgatgc ggtaatcgtc
     8761 aacggcggtg tccctcgcag cggtgaaatc cccatgcatc tgctgccggg tcaggcggtg
     8821 aatttacgta ccggtacgcg gtgtgacgtt gctcagcttg aacatgtagt ggtgatggcg
     8881 gggattgggc atccgccgcg cttttttgcc acgctgaaga tgtgtggcgt acaaccggaa
     8941 aaatgtgtac cgctggccga tcatcagtct ttgaaccatg cggatgtcag tgcgttggta
     9001 agcgccgggc aaacgctggt aatgactgaa aaagatgcgg tgaaatgccg ggcctttgca
     9061 gaagaaaatt ggtggtattt gcctgtagac gcacagcttt caggtgatga accagcgaaa
     9121 ctgcttacgc aactaacctc gctggcttct ggcaactagt tacgccgcgg cagcgttcga
     9181 ttgatggagt catgaatgtc gctgccgcac ctctcccttg ctgatgcgcg taatcttcac
     9241 cttgccgcac aaggcctgtt aaacaaaccc cgccgtcgag cgtcgttgga ggatattccg
     9301 gcaacgatct cccgcatgtc cttgctgcaa atcgatacca tcaatattgt tgcccgtagt
     9361 ccatatctgg tgcttttcag tcgtctggga aattatcctg cccagtggct ggatgagtct
     9421 ctggcgcgtg gcgaattaat ggaatactgg gcgcatgaag cctgctttat gccgcgtagc
     9481 gactttcgtc ttattcgcca ccgcatgctg gcacctgaaa aaatgggctg gaaatacaaa
     9541 gacgcctgga tgcaggaaca tgaggcggaa attgcacagt taattcagca tattcatgat
     9601 aaagggccgg tacgttcagc cgattttgag catcctcgta aaggtgcaag cggctggtgg
     9661 gaatggaagc cgcataaacg gcatctggaa ggtttattta ctgccggaaa ggtgatggtg
     9721 attgaacggc gcaacttcca gcgcgtttat gatttaaccc accgtgtcat gcctgactgg
     9781 gatgatgagc gcgatctcgt ttcgcaaaca gaagcagaaa tcatcatgct ggataacagt
     9841 gcgcgtagcc tgggaatatt ccgcgaacag tggctggcag attactatcg gctgaaacgt
     9901 ccggcactgg cggcgtggcg cgaagcgagg gctgaacagc agcaaatcat tgctgtgcat
     9961 gttgaaaaat tgggcaatct ttggctgcat gctgatttgc tgccgctact cgagcgtgcg
    10021 ctggccggaa agctcactgc aacgcacagc gcggtacttt cgccttttga tcctgttgtc
    10081 tgggatcgca aacgcgcaga gcagcttttt gattttagct accggctgga gtgctatacc
    10141 ccagcgccga aacgccagta tggctatttt gttctgccat tattacatcg tgggcaatta
    10201 gttgggcgaa tggatgccaa aatgcatcgc cagacaggca tccttgaagt tatctctctg
    10261 tggttacagg aaggcattaa accaacgaca atgctgcaaa aagggttacg tcaggcgatt
    10321 actgatttcg cgagctggca gcaggcaacg cgggtgacat taggacgctg cccgcaaggt
    10381 ctctttactg attgccgcac tggctgggaa atagaccccg tcgcataaat gattatgata
    10441 agatttatgc attcataacc cgatccatct ggaggaacta tggatcatcg tttgcttgaa
    10501 atcattgcct gcccggtttg caacggaaaa ctttggtata accaggaaaa acaagagctt
    10561 atttgcaaac tggataacct ggctttcccg ctccgcgatg gcattccagt gttgctggaa
    10621 acggaagccc gcgtgctgac tgctgatgag agtaaatcat gagttttgtg gtcattattc
    10681 ccgcgcgcta cgcgtccacg cgtctgcccg gtaaaccatt ggttgatatt aacggcaaac
    10741 ccatgattgt tcatgttctt gaacgcgcgc gtgaatcagg tgccgagcgc atcatcgtgg
    10801 caaccgatca tgaggatgtt gcccgcgccg ttgaagccgc tggcggtgaa gtatgtatga
    10861 cgcgcgccga tcatcagtca ggaacagaac gtctggtgga agttgtcgaa aaatgcgcat
    10921 tcagcgacga cacggtgatc gttaatgtgc agggtgatga accgatgatc cctgcgacaa
    10981 tcattcgtca ggttgctgat aacctcgctc agcgtcaggt gggtatggcg actctggcgg
    11041 tgccaatcca caatgcggaa gaagcgttta acccgaatgc ggtgaaagtg gttctcgacg
    11101 ctgaagggta tgcactgtac ttctcacgtg ccaccattcc ttgggatcgt gatcgttttg
    11161 cagaaggcct tgaaaccgtt ggcgataact tcctgcgtca tcttggtatt tatggctacc
    11221 gtgcaggctt tatccgtcgt tacgtcaact ggcaggcaag tccgttagaa cacatcgaaa
    11281 tgttagagca gcttcgtgtt ctgtggtacg gcgaaaaaat ccatgttgct gttgctcagg
    11341 aagttcctgg cacaggtgtg gatacccctg aagatcttga gcgcgttcgc gctgaaatgc
    11401 gctaatttca cttcacgaca cttcagccaa ttttgggagg agtgtcgtac cgttacgatt
    11461 ttcctcaatt tttcttttca acaattgatc tcattcaggt gacatctttt atattggcgc
    11521 tcattatgaa agcagtagct tttatgaggg taatctgaat ggaacagctg cgtgccgaat
    11581 taagccattt actgggcgaa aaactcagtc gtattgagtg cgtcaatgaa aaagcggata
    11641 cggcgttgtg ggctttgtat gacagccagg gaaacccaat gccgttaatg gcaagaagct
    11701 ttagtacgcc cggaaaagcc cgacaactgg catggaaaac caccatgctg gcaagaagtg
    11761 ggactgtccg tatgccgact atttatggtg tgatgacgca tgaagaacac cccggccctg
    11821 atgtcctgct actggagcgg atgcgtggtg tttcggtgga ggcaccagcc cgaacaccag
    11881 aacgctggga acagctcaaa gaccaaatcg ttgaagcctt actggcctgg caccgtcagg
    11941 acagtcgcgg ttgcgtcggc gcggtcgaca atactcagga aaatttctgg ccctcatggt
    12001 accggcaaca tgttgaagtg ctatggacca cgctcaatca gttcaataac accggtctga
    12061 cgatgcagga taagcggatc ctgtttcgca ctcgcgaatg tctcccggca ttatttgaag
    12121 gttttaacga caattgtgtg ttgattcacg gtaacttctg tttacgcagc atgctgaaag
    12181 attcgcgcag cgatcagtta ctggcgatgg tcgggccggg actaatgctt tgggcaccgc
    12241 gagaatatga actgttccga ctaatggata attctctggc ggaagatttg ctctggagtt
    12301 acctgcaacg cgcgccagtg gcggagtcgt tcatctggcg gcgttggttg tatgtgttat
    12361 gggatgaagt tgcgcaactg gttaataccg gacgatttag tcggcgcaac ttcgatctgg
    12421 catcaaaatc actcttgccg tggctcgcct gacggacctt tcagccactg ccagatgcgg
    12481 ccaagcgttt catagccaac acgatcgcta tgcatcagcc agaccggaga agggattgcc
    12541 cgttcccacg gattaagcgg cgagtcgatg gccaactgat tcgccggggc tggtagcgga
    12601 tttaaccctt cctgctgaaa aaagatcatc gcccgcggca gatgtgatgc ggaggtcacc
    12661 agcaggaaag gggcgtcacc aatcgcctgt ttcactgccg cagcttcttc ttcggtatct
    12721 tttggcaaat ccagggtgat aatttgctcg cgcggtacgc ccagcgattg cgcaactctg
    12781 gtacctactt ccgctgtact caccgtattg gtttttgcta cgcctcccgt gaagatcagt
    12841 tttgaacccg gattttcgcg ccataaacgg ataccctcat tcaggcgtgg cagactgtta
    12901 ttgattaaat tagagctcgg tgcccactgc gggttccagg tataaccacc tccgagcacc
    12961 acgatatagt ccactttttg cgaattattc catgtcggat aggtgctttc gatgggacgc
    13021 agcaggcggt cggctaccgg ttgcaaactc aataacaaga gtgccagcca cccaatgcta
    13081 ataaaaatct tcccggtttt ttgaaaacgg ctaaaccaca gcagcgccag gccagcgcca
    13141 ataattaaca acatcagcgg aagtggcagc aacatattgc caatcacttt tttcagtgta
    13201 aaaagcatcc tttctggttc cttttttaac catatagcaa gggatcgcca gcgatattac
    13261 accagacggg ttcattctcg ccgtggctgt gacaaaatag cgtctttatc agaaaacgtc
    13321 ttagccaacc ggtggagaaa gcaatgcagg atcgcaattt tgatgatatt gcggaaaagt
    13381 tttcccgtaa catttacggc accaccaaag ggcagcttcg acaggctatt ctgtggcagg
    13441 atctcgatcg cgtgctggcg gaaatgggcc cgcaaaaact gcgtgtgctg gatgctggcg
    13501 gcggagaagg gcagaccgca atcaaaatgg ccgagcgcgg gcatcaggtt attttatgcg
    13561 atctttctgc gcagatgatc gaccgcgcaa aacaggcggc agaagcaaaa ggtgtgagcg
    13621 acaacatgca atttatacat tgcgccgctc aggatgttgc ttcgcatttg gaaacgcccg
    13681 ttgatctgat attgttccat gcggtgctcg agtgggtggc cgatccccgc agtgtattgc
    13741 agaccctgtg gtcagtgttg cgtccaggca gagtgttgtc gttaatgttc tacaatgcgc
    13801 atggtttgtt gatgcataac atggtcgccg ggaattttga ttacgtgcag gcgggaatgc
    13861 cgaaaaagaa aaaacggacg ctttcgccag attatccacg cgacccggcg caggtttatc
    13921 tgtggctgga agaagctggt tggcaaatta tgggtaagac aggcgttcgc gtgtttcatg
    13981 attatctgcg cgagaagcac cagcagcgcg actgctatga agcattactt gaattagaaa
    14041 cgcgttattg ccgtcaggaa ccgtatatta ccctagggcg ttatattcat gtcaccgcgc
    14101 gcaaaccgca gagcaaggat aaagtatgag tgaattttcc cagacagtcc ccgaactggt
    14161 tgcctgggcc agaaaaaatg acttctccat ctcgctgccg gtagaccgac tctcttttct
    14221 gctggcggtt gccacgctga acggcgagcg tctggatggt gagatgagtg aaggcgagct
    14281 ggtggatgca ttccgccatg tgagtgatgc gtttgagcaa accagcgaaa ccatcggcgt
    14341 gcgcgccaat aacgcgatca acgacatggt gcgtcaacgt ctgctgaacc gctttaccag
    14401 cgagcaggcg gaagggaacg caatttaccg tctgacgccg ctcggcatcg gcattactga
    14461 ctactacatc cgtcagcgcg agttttctac gctgcgtctt tctatgcagt tgtcgattgt
    14521 ggcgggtgag ctcaaacgcg cagcagatgc cgccgaagag ggcggtgatg aatttcactg
    14581 gcaccgtaat gtctatgcgc cactgaaata ttcggtagca gaaattttcg acagtatcga
    14641 cctgacgcaa cgtctgatgg acgaacagca gcagcaggtg aaggacgata tcgcccagtt
    14701 gctgaacaaa gactggcggg cggcgatttc cagctgtgaa ttgttgcttt cggaaacttc
    14761 cggaacgctg cgtgaattgc aggatacgct ggaagcggca ggcgacaaat tgcaggctaa
    14821 tctgttgcgc attcaggatg cgacgatgac ccatgacgat ctgcatttcg tcgatcgtct
    14881 ggtgttcgat ctgcagagca aactcgatcg tattatcagt tggggccagc aatccatcga
    14941 cttgtggatt ggctacgacc gccacgtaca caaatttatt cgtaccgcga tcgatatgga
    15001 taaaaaccgc gtctttgctc agcggttacg tcagtcggta caaacctatt ttgatgagcc
    15061 gtgggcgcta acttatgcca atgccgatcg tctgctggat atgcgtgacg aagagatggc
    15121 actgcgcgat gaagaagtga ctggggaact tcctgaggat ctggaatacg aagagtttaa
    15181 cgagatccgc gaacagctgg cggcgatcat cgaagaacaa cttgccgtgt acaaaaccag
    15241 acaagtgccg ctggatcttg gtctggtggt acgcgaatat ctgtcacagt atccgcgtgc
    15301 acgtcacttt gacgttgcgc gtattgttat tgatcaggcg gtacgtcttg gcgtagcgca
    15361 agcagatttc accggactgc cagcgaaatg gcagccgatt aatgattacg gagccaaggt
    15421 acaggcgcat gtcatcgaca aatattgaac aagtgatgcc ggttaagctg gcgcaggcgc
    15481 tggcgaatcc gttatttccg gcgctggaca gcgccttacg ttcaggccgc catattggcc
    15541 tcgacgaact ggataatcat gcattcctga tggattttca ggaatatctg gaagagtttt
    15601 acgcgcgtta taacgttgag cttattcgcg caccggaagg gttcttctat ttacgcccac
    15661 gttccaccac tcttatcccg cgttccgtct tgtcggaact ggatatgatg gtcgggaaaa
    15721 tcctctgtta tctctatctc agcccggaac ggctggcgaa tgaggggatt ttcacccagc
    15781 aggaactgta cgacgaactg ctcaccctgg ccgatgaagc gaaactgctg aaactggtga
    15841 acaaccgttc aaccggttca gacgttgacc gtcagaagtt gcaggagaaa gtacgttctt
    15901 cgctcaaccg tctgcgtcgt ttaggcatgg tgtggtttat gggccacgac agcagcaagt
    15961 tccgcattac cgaatcggtg ttccgcttcg gagccgatgt gcgtgctggc gacgatcccc
    16021 gtgaagcaca gcgtcgcctg attcgtgatg gcgaagcaat gccgattgaa aatcatctgc
    16081 aactcaacga tgaaaccgaa gagagtcagc cagatagcgg agaggaagaa taatgattga
    16141 acgcggtaaa tttcgctcac tgacgctgat taactggaac ggcttttttg cccgaacttt
    16201 tgaccttgac gagctggtca cgacgctttc cggcggtaac ggggcgggta aatccaccac
    16261 gatggcggcg ttcgttacgg cgctgatccc cgacctgacc ctgctgcatt tccgtaacac
    16321 tacggaagcc ggggccacca gcggttcgcg cgataaaggt ctgcacggta agctgaaagc
    16381 gggtgtctgt tattcgatgc tcgacaccat taactcgcgc caccagcgcg tggtggtcgg
    16441 tgtgcgtctg caacaggttg ccggacgcga tcgtaaagtg gatatcaaac catttgccat
    16501 tcagggactg ccgatgtcgg tgcagccgac acagctggtg accgaaaccc tgaacgaacg
    16561 ccaggcgcgc gtgctgccgc ttaacgagct gaaagacaag ctcgaggcga tggaaggcgt
    16621 gcagtttaaa cagttcaact ccattaccga ttaccactcg ctgatgttcg atctgggcat
    16681 catcgcgcgt cgtctgcgct ccgcatctga ccgtagcaaa ttctatcgtc tgatcgaagc
    16741 ttcgctgtat ggcgggatct ccagtgccat tacccgttct ctgcgcgact acctgttgcc
    16801 ggaaaacagc ggcgtgcgta aagcgttcca ggacatggaa gcggcgctgc gtgaaaaccg
    16861 catgacgctg gaagcgattc gtgtcaccca gtcggaccgt gacctgttta agcatctgat
    16921 cagcgaagct accaactacg tggcggcgga ctacatgcgt cacgccaacg agcgccgtgt
    16981 ccatctcgac aaagccctgg agtttcgtcg cgagctacat acttcgcgtc agcaactggc
    17041 ggctgagcag tacaaacacg tcgatatggc gcgtgagctg gcagagcaca acggtgccga
    17101 aggcgatctg gaagcggatt atcaggcggc cagcgatcac ctgaacctgg tgcaaaccgc
    17161 attgcgtcag caggagaaga tcgaacgcta cgaagtggat ctcgatgagc tgcagatccg
    17221 tctggaagag caaaatgaag tggtggcaga agccatcgaa cgccaggaag agaatgaggc
    17281 tcgcgcggaa gctgccgaac tggaagtgga cgagctgaaa agccagcttg ctgactacca
    17341 gcaggcgctg gacgtccagc aaacgcgcgc gatccagtat aaccaggcga ttgctgcgct
    17401 taatcgtgcc aaagaactgt gccatctgcc ggacttaacc gccgacagcg ccgccgaatg
    17461 gctggaaacc ttccaggcga aagagctgga agcgactgaa aaaatgctct ctcttgagca
    17521 gaaaatgagc atggcgcaaa ccgcgcacag ccagtttgag caggcttatc agctggtggt
    17581 ggcaatcaac ggcccgctgg cgcgtaacga ggcgtgggat gtcgctcgcg aactattgcg
    17641 cgaaggggtt gatcagcgtc acctggcaga gcaggttcag ccgttgcgga tgcgcttaag
    17701 cgaactggaa cagcgtctgc gtgagcagca agaagctgag cgtctgctgg cagatttctg
    17761 caaacgtcag ggcaagaatt ttgatattga tgaactggaa gctctgcatc aggagctgga
    17821 agcacgcatt gcctctcttt ccgatagcgt atctaacgcc cgtgaagagc ggatggcact
    17881 gcggcaggaa caggaacagc tgcagtctcg cattcagagt ttgatgctgc gtgcaccggt
    17941 ttggctggca gcgcaaaaca gtctcaacca gttgagtgaa cagtgcggcg aagagttctc
    18001 ctccagccag gacgtcactg aatatctgca acagttgctg gagcgtgagc gcgaggcgat
    18061 tgttgaacgc gatgaagtag gcgcgcgcaa aaacgccgtc gatgaagaga tcgaacgttt
    18121 aagccagcct ggcggctctg aagatcagcg tctgaacgcg ctggcggagc gttttggtgg
    18181 tgtgctgctg tcagaaattt atgacgacgt tagcctggaa gatgcgccgt acttctcggc
    18241 tctgtatggc ccgtcacgcc acgccatcgt ggtgccagat ctgtcacagg taaccgaaca
    18301 cctggaaggc ttgaccgatt gcccggaaga tctctatttg atcgaagggg atccgcagtc
    18361 attcgatgat agcgtgttca gcgttgatga gctggaaaaa gcggtggtgg tgaaaatcgc
    18421 cgatcgacag tggcgttatt cacgtttccc ggaagtgccg ctgtttggtc gtgctgcgcg
    18481 tgaaagccgt attgaaagcc tccatgccga gcgtgaagtg ctttccgaac gcttcgccac
    18541 gctctccttt gatgtacaga aaacccagcg tttgcatcag gcgttcagcc gctttatcgg
    18601 cagtcatctg gcggtggcgt ttgagtctga cccggaagca gaaatccgtc aactgaacag
    18661 ccgtcgagtc gaactggagc gggcgttgag taatcatgaa aatgataacc agcagcagcg
    18721 tattcagttt gagcaggcga aagaaggcgt tacggcgctg aaccgcattc tgccgcgtct
    18781 caacctgttg gctgatgaca gcctggcgga tcgcgtcgat gaaatccgcg aacgtctgga
    18841 tgaagcccag gaagccgcgc gttttgttca gcagtttggc aatcaactgg cgaaactgga
    18901 accgatcgtt tcggtattgc agagcgaccc ggaacagttc gaacagttaa aagaagatta
    18961 cgcgtactct cagcagatgc agcgcgatgc ccgtcagcaa gcgtttgccc tgacggaagt
    19021 ggtgcagcgt cgtgcgcact ttagctattc tgactcggca gaaatgctta gcggtaacag
    19081 cgatctcaac gaaaaactgc gtgaacgtct ggaacagtcg gaagcggagc gtacccgcgc
    19141 tcgcgaagcg ttgcgcggtc acgcagcgca gttgagtcag tacaaccagg tgctggcttc
    19201 gctgaaaagt tcttacgaca ccaaaaaaga gctactcaac gatctgcaac gtgaattgca
    19261 ggatatcggc gtgcgtgctg atagcggggc agaagagcgg gcgcgtattc gccgtgacga
    19321 gctgcatgcg caactgagca ataaccgttc acgccgcaat caactggaaa aagcgcttac
    19381 cttctgcgaa gcggagatgg acaacctgac ccgcaaactg cgcaagctgg agcgggatta
    19441 ctttgagatg cgcgagcagg tagtgaccgc caaagcgggc tggtgtgcgg tgatgcgcat
    19501 ggtgaaagat aacggcgttg agcgccgctt acaccgtcgt gagctggctt atctctccgc
    19561 tgatgatttg cgttccatgt cggataaggc gttaggtgcg ctgcgtctgg cggtggcgga
    19621 taacgaacat ctgcgcgacg tgctgcgcat gtcggaagat ccgaaacgtc cggagcgtaa
    19681 aattcagttc ttcgtggcgg tttatcagca tctgcgtgaa cgtattcgtc aggatattat
    19741 tcgtaccgat gatccggtgg aagctatcga acagatggag attgaactta gccgtctgac
    19801 cgaagaatta acctcccgtg aacagaaact ggcgatcagt tcccgcagcg tggcgaacat
    19861 cattcgcaaa accattcagc gcgagcagaa ccgtatccgt atgctcaacc aggggttgca
    19921 gaacgtatcg tttggtcagg tgaacagcgt gcgtctcaac gtgaacgtgc gtgaaacgca
    19981 cgccatgctg ctggatgtgc tctctgaaca gcacgagcag catcaggatc tgtttaacag
    20041 caaccgtctg accttctcgg aagcgttggc gaaattgtat cagcgtctta atccgcagat
    20101 tgatatgggg cagcgcacgc cgcagaccat tggcgaagaa ctgctggatt accgcaacta
    20161 tctggaaatg gaagttgagg ttaaccgtgg ttccgatggt tggttgcgcg cagagtctgg
    20221 tgcattgtcg accggtgagg cgattggtac cggtatgtcg attctggtga tggtggtaca
    20281 aagctgggaa gatgaatctc gccgcctgcg cggtaaagat atctctcctt gccgcctgct
    20341 gttcctcgat gaagcagcgc gactggatgc tcgttctatc gccacgctgt ttgaattgtg
    20401 tgaacgtttg caaatgcagc tcatcatcgc agcgccggaa aatatcagtc cggaaaaagg
    20461 caccacctat aaactggtac gtaaagtctt ccagaatacc gaacatgttc acgtcgtcgg
    20521 cctgcgagga tttgcgccgc aactccctga aacgcttcca ggaagagacg aagcgccttc
    20581 ccaggcgagt taaaattaag gcggcagcaa tgccgccttt tctttttccg aaaactccgt
    20641 ttctgcacta aaaaaatggc acataacggc gtaatgc
//
LOCUS       DAJTYG010000067        20632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTYG010000067 DAJTYG010000000
VERSION     DAJTYG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.067"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO568_002624"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO568_002624"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6641933.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OO568_002625"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OO568_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6641934.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="OO568_002626"
     CDS             complement(2649..3200)
                     /locus_tag="OO568_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6641935.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="OO568_002627"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="OO568_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6641936.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="OO568_002628"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="OO568_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR6641937.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OO568_002629"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OO568_002629"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6641938.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OO568_002630"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OO568_002630"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6641939.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OO568_002631"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OO568_002631"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6641940.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHTESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVTWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKFIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OO568_002632"
                     /pseudo
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OO568_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OO568_002633"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OO568_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6641941.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTPGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="OO568_002634"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="OO568_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6641942.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="OO568_002635"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="OO568_002635"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6641943.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OO568_002636"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OO568_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6641944.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OO568_002637"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OO568_002637"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6641945.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OO568_002638"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OO568_002638"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6641946.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKNKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OO568_002639"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OO568_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6641947.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="OO568_002640"
     CDS             complement(18174..18569)
                     /locus_tag="OO568_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6641948.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTEVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OO568_002641"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OO568_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6641949.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="OO568_002642"
     CDS             19485..20078
                     /locus_tag="OO568_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6641950.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..>20632
                     /gene="tabA"
                     /locus_tag="OO568_002643"
     CDS             20223..>20632
                     /gene="tabA"
                     /locus_tag="OO568_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6641951.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAP"
BASE COUNT         4904 a         5199 c         5472 g         5057 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc ggatggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgcta gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggctcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaaa catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtagag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 ctttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaag ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gatgaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgtcac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgt atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tcgagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg ggtggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gctatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccgggc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgaccttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acacaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctccgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg atcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc caggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg ttcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac gcctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggaaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 acctccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttaccggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatagtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagc ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcggggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacattg ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag cacgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg cc
//
LOCUS       DAJTYG010000068        20376 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTYG010000068 DAJTYG010000000
VERSION     DAJTYG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.068"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OO568_002644"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OO568_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6641952.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OO568_002645"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OO568_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR6641953.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="OO568_002646"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="OO568_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6641954.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO568_002647"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO568_002647"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6641955.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO568_002648"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO568_002648"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6641956.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="OO568_002649"
     CDS             4449..4814
                     /locus_tag="OO568_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6641957.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISD
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4964..5482
                     /locus_tag="OO568_002650"
     CDS             4964..5482
                     /locus_tag="OO568_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6641958.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="OO568_002651"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="OO568_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="OO568_002652"
     CDS             6713..7195
                     /locus_tag="OO568_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6641959.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO568_002653"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO568_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6641960.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO568_002654"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO568_002654"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6641961.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO568_002655"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO568_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6641962.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO568_002656"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO568_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6641963.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO568_002657"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO568_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6641964.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="OO568_002658"
     CDS             10939..11730
                     /locus_tag="OO568_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6641965.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="OO568_002659"
     CDS             11751..13037
                     /locus_tag="OO568_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6641966.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO568_002660"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO568_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6641967.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="OO568_002661"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="OO568_002661"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6641968.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="OO568_002662"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="OO568_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6641969.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="OO568_002663"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="OO568_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6641970.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="OO568_002664"
     CDS             complement(16985..17275)
                     /locus_tag="OO568_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6641971.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO568_002665"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO568_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6641972.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="OO568_002666"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="OO568_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6641973.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="OO568_002667"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="OO568_002667"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20376
                     /locus_tag="OO568_002668"
                     /pseudo
     CDS             19137..20376
                     /locus_tag="OO568_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         5261 a         5324 c         4781 g         5010 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcgct ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc agtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcattaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgt gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acaccccctt tgaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcggac agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaggggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gccttatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgttgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaattagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgtcttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcgacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcta
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctggtgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gctgtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttagacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtgccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg cgagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aagctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgcat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatctc tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttga ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtactgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgccccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgccagtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatag tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggt
//
LOCUS       DAJTYG010000069        19918 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTYG010000069 DAJTYG010000000
VERSION     DAJTYG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.069"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..746)
                     /locus_tag="OO568_002669"
     CDS             complement(<1..746)
                     /locus_tag="OO568_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6641974.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSY
                     MWFETGDNGNEYFKWRHHLTGGRVKDLMNLKWDALYVLVKALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLVVTGDIF
                     TNRFLINSSTGMWIHMRDQNVIMGRNAVSNDGAQALLRQDHTDRKFVIGGLGNRQFGI
                     YMINNSRTANGTDGQAYMDNNGNWLCGSQV"
     gene            840..1037
                     /locus_tag="OO568_002670"
     CDS             840..1037
                     /locus_tag="OO568_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6641975.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTRTDRPG
                     LKRALKRLQKGTHIENDDRIN"
     gene            1131..1319
                     /locus_tag="OO568_002671"
     CDS             1131..1319
                     /locus_tag="OO568_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6641976.1"
                     /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            1270..1965
                     /locus_tag="OO568_002672"
     CDS             1270..1965
                     /locus_tag="OO568_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HCR6641977.1"
                     /translation="MGKEKKSRILTKPCVIEYEGQIVGYGSKELRVETISCWLARTII
                     QTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETDNRGYMELNRM
                     WLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGLAGVVYQASNFDFIGSH
                     ESTFYELDGEWYHEITMNAIKRGGQRGEYLRANKERAVVHKFNQYRYIRFLNKRARKR
                     LNTKLFKVQPYPK"
     gene            complement(2077..2424)
                     /locus_tag="OO568_002673"
                     /pseudo
     CDS             complement(2077..2424)
                     /locus_tag="OO568_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2393..4050)
                     /gene="dpiB"
                     /locus_tag="OO568_002674"
                     /pseudo
     CDS             complement(2393..4050)
                     /gene="dpiB"
                     /locus_tag="OO568_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            4431..5456
                     /gene="citC"
                     /locus_tag="OO568_002675"
     CDS             4431..5456
                     /gene="citC"
                     /locus_tag="OO568_002675"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6641978.1"
                     /translation="MFVNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKLRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWFHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSR"
     gene            5504..5800
                     /gene="citD"
                     /locus_tag="OO568_002676"
     CDS             5504..5800
                     /gene="citD"
                     /locus_tag="OO568_002676"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6641979.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5797..6705
                     /gene="citE"
                     /locus_tag="OO568_002677"
     CDS             5797..6705
                     /gene="citE"
                     /locus_tag="OO568_002677"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6641980.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6716..8248
                     /gene="citF"
                     /locus_tag="OO568_002678"
     CDS             6716..8248
                     /gene="citF"
                     /locus_tag="OO568_002678"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6641981.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8252..8803
                     /gene="citX"
                     /locus_tag="OO568_002679"
     CDS             8252..8803
                     /gene="citX"
                     /locus_tag="OO568_002679"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR6641982.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8778..9656
                     /gene="citG"
                     /locus_tag="OO568_002680"
     CDS             8778..9656
                     /gene="citG"
                     /locus_tag="OO568_002680"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6641983.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9707..11170
                     /gene="citT"
                     /locus_tag="OO568_002681"
     CDS             9707..11170
                     /gene="citT"
                     /locus_tag="OO568_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR6641984.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMDKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNML
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11284..12090
                     /gene="rna"
                     /locus_tag="OO568_002682"
     CDS             11284..12090
                     /gene="rna"
                     /locus_tag="OO568_002682"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6641985.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12319..12729
                     /gene="rnk"
                     /locus_tag="OO568_002683"
     CDS             12319..12729
                     /gene="rnk"
                     /locus_tag="OO568_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6641986.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLRDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(12960..14197)
                     /locus_tag="OO568_002684"
                     /pseudo
     CDS             complement(12960..14197)
                     /locus_tag="OO568_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            14418..14846
                     /gene="uspG"
                     /locus_tag="OO568_002685"
     CDS             14418..14846
                     /gene="uspG"
                     /locus_tag="OO568_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6641987.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14967..16532)
                     /gene="ahpF"
                     /locus_tag="OO568_002686"
     CDS             complement(14967..16532)
                     /gene="ahpF"
                     /locus_tag="OO568_002686"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6641988.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16777..17340)
                     /gene="ahpC"
                     /locus_tag="OO568_002687"
     CDS             complement(16777..17340)
                     /gene="ahpC"
                     /locus_tag="OO568_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6641989.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17712..18458
                     /gene="dsbG"
                     /locus_tag="OO568_002688"
     CDS             17712..18458
                     /gene="dsbG"
                     /locus_tag="OO568_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6641990.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18668..19570
                     /locus_tag="OO568_002689"
     CDS             18668..19570
                     /locus_tag="OO568_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6641991.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            19717..19918
                     /locus_tag="OO568_002690"
                     /pseudo
     CDS             19717..19918
                     /locus_tag="OO568_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         5020 a         5128 c         4755 g         5015 t
ORIGIN      
        1 ataacttgcg aaccgcaaag ccagttcccg ttattatcca tgtacgcctg accatcggtg
       61 ccattggctg tccttgagtt attaatcatg tagatgccaa attgcctatt ccccagccca
      121 ccaattacga atttacggtc ggtatggtcc tggcgaagca atgcctgcgc accatcgttg
      181 gataccgcat tacggcccat aataacgttc tggtcacgca tatgaatcca catgcctgta
      241 ctgctgttaa ttaaaaaacg gtttgtaaat atatctcctg taacaaccag accatgcccc
      301 atgcttatcc ggccagttct gagattaagc gtaaaggggc gtagtggccc tatattacca
      361 ttttcccctt cattctctcg tgtagggatg atatgcaggc attcttcaga acgacgaaaa
      421 atggcaccaa aagatgaatt aaatattctc agtgcattga ccgtcgatat ttttacttca
      481 ctgctgaaaa gggctttaac aagaacatac agagcatccc atttaagatt catcaggtct
      541 ttaacccggc cgccagtgag gtggtgtctc catttgaaat actcattgcc gttgtcgcct
      601 gtttcaaacc acatgtatga atcagtatcg ctgtcggcat catttttaaa tccaatcttt
      661 gcccagtcag tattccgaat ccaggcaagg attgagtcgt tttcaaaagt aagtccaccg
      721 gacaacgtat cgccattttt ttgcacggcg ttaccagcct tgtttaccgt ttcctgtaaa
      781 ccgaggggcc gtttctggcc tgcatggcat gatttccgct tttaaacggg gaattcagcg
      841 tgctgattgg ctatgtaagg gtatcaacaa atgaccagaa tacagacctg caacgaaacg
      901 ctcttgtttg tgcaggatgt gaacaaatat ttgaagataa attaagcgga acaaggacag
      961 accgaccggg attaaaacgt gctttaaagc gccttcaaaa aggaacgcat atagaaaacg
     1021 acgatcgaat caattaaatc gatctatatt accgatcgat tatgtcccga taaccacacc
     1081 cgactgatga gattttttaa gataacggcg aattgatgca aggaggtgaa atgaaatcaa
     1141 ttcgctgtaa gaactgcaac aaactgttat ttaaagcgga ttcctttgat cacattgaaa
     1201 tcaggtgtcc gcgttgcaaa cgtcacatca taatgctgaa tgcctgcgag catcccacgg
     1261 agaaacattg tgggaaaaga gaagaaatca cgcattctga cgaaaccgtg cgttattgag
     1321 tatgaaggcc agattgttgg ctatggttca aaggagctgc gcgttgaaac catatcctgc
     1381 tggctggccc gcacaatcat tcagacaaag cactattccc gccgttttgt gaataactct
     1441 taccttcacc tgggagtatt cagcgggcgt gatctggttg gcgttctcca gtggggatat
     1501 gcccttaacc ccaactcagg tcgtcgtgtc gtgcttgaaa cggataaccg gggctatatg
     1561 gagttgaatc gcatgtggct acacgacgac atgccccgca actctgaatc acgggccatc
     1621 agctacgcgc tgaaagttat cagattactg tatccgtcag tggagtgggt tcagtccttt
     1681 gctgacgaac gctgcggact cgcaggcgtt gtgtatcagg cgtcgaattt tgattttatt
     1741 ggcagccatg aaagcacgtt ctacgagctg gatggtgaat ggtatcacga gataacgatg
     1801 aacgcgatta agcgcggagg gcaacgaggc gagtatttgc gagctaataa ggagcgtgcc
     1861 gtggtgcaca aatttaatca gtatcgctac atcagattcc tgaacaagcg agcaaggaag
     1921 cggctaaata ccaaactatt caaggttcag ccatacccta agtgatcccc atgtaatgaa
     1981 taaaaagcgg taattaatac tgctgtttca tttgaagcgc gaaagctaaa gttttcgcat
     2041 ttatcgtgaa acgctttcgc gtttttcgtg cgccgcttca cccagccgtt cataggcaat
     2101 tggcttaatg agataatcaa atacaccaca acgtacggct tcagacaccg tttccatatc
     2161 actggctgca gtggtaaaca ccacgtcgcc gggataatgc gcctgcacca gttcatgcag
     2221 taaattaatc cctctaccgt caggaagata gttatcgagc aagattagcc ccggcttaaa
     2281 acgctcgatc atcattcggg cctgcgccag gtttcccgcc agtaatatct gactgaatcc
     2341 gggaatgtga cgaatatatt ccgcatgcat ctctgccagc ggcgtttcgt cctcaacgat
     2401 caatagggtt aatggagctg tcattaggtt tcactttcgg aatatagatt gaaaataagg
     2461 taccgcaggg atcattatct tcgagagtga taacaccacc gcagcgcgtt acgtagctgg
     2521 caatcaagta caacccaatg ccatgttcac cgggctcgtc agcacgcgta ctgacccctg
     2581 ctcaaatatt ttgtctcgta gagactctgg aacgccgcag ccctgatcgg cgacttcaat
     2641 caccacatca tcgccttcat cgctgaggaa taattcaacg ctcttgtttc cttcatcgct
     2701 acgcaggctg gcttcgaagg cgttatcaag taaattgcca acaatggcgg caaactcggt
     2761 gctatccagt cctggcggca gttgcgaaag ctggctaccg gggacgatga tcatttttag
     2821 ccccagttcg cgggcgcgct gcactttacc aaaaagcagc cccgccacct ggcgatcggc
     2881 aaacgcctcg cgcaggctgt caataagctg ttgctgggcc tgagactccc cctgcaccat
     2941 cgccagcacg cgatcatact ctttcatctg caacagacca ttgagagtcg acatccaatt
     3001 gagatgctcg tgtcgcaatg tacgaaggct ctcaacgtat tgttttattt gcgtcagttg
     3061 cgcattgagg gtggaaattt cgtctttact gcgaaagctg ataatggctc ccagcaaatc
     3121 atcacctgaa cgaatagctt cccgattggc aataacgctc agaccgttaa agttagccac
     3181 cacatcctga cgtttttcat caatctgttc ggtaaagaaa tcggcagacc tgaccacttc
     3241 agcaatgggt ttacccaacc attgccgtcc aggggaactc agccccagca tctttcttgc
     3301 gttacgattg atggcggtaa tgtaaccgtg cggatccacc gcaatcagcc cttcataaac
     3361 cgaactaaac agcgcctctt gctgacggac cacccgtgcg atttgctttg gctccatgcc
     3421 catcatctgc cgacggatat gcgcggccag gaaccacgac agcaacatca gaatccctaa
     3481 cagcacgaca aacacacctg ccatcggtaa taaaaactca gcccgccagc tatcgatttt
     3541 actcaccagg tagccaatcg acaccacgcc gatgactttt ccatcgtcat caaagattgg
     3601 cgttttggcg cgcatcgcca tacccattga ccctttcccg gtaatgaagt agctctcccc
     3661 tttctccagc gcgccctgtt tggtgaactg cataggataa ccaattttct ccggattagg
     3721 atggtaaagg cggatcgagt gccggtcgcc aatcaccaca taatcaaaat cggtatctct
     3781 ttgtaatttg ttagcgatgg tcgccagccg tttgtagtca cgcgttttca ccgcagagat
     3841 gacactgtca ttggaggcaa taattttcgc ctgattcatt gccatgtcgc gtacatgaag
     3901 cgttaaatag tcctcaaaac tggccgtaaa atattgcgcc agggctgcaa tgacaaatat
     3961 tgagaacacc agaatcagca aaaagatacg cagcggaaat gtcagtcttt ggaaaaatgc
     4021 aaactgttta ttctcgttaa gctgcaacat tgttttcctt gcgggttgtc ccgagcctct
     4081 ttgacggatg taattaaaat gttgaatcaa aatacataga tattaattat gatcgtggca
     4141 aattttattc attaaatcaa ttaaatcact taaacctgcg agtcatttcg taattacatt
     4201 aattaaaaaa cttaaaacca ttaaacaaat aaaaaaacca ctaaccctat gtgaaataaa
     4261 tcaaaatttc acgccgaaat actccttagg atgtatagcg aaaggagaaa aagatatacc
     4321 tcgatcaccc ccctttctcc caagtgaaaa taaaaggtta tcagtttgca acattgaaca
     4381 acattcgttg caaatcgata acaacatgca ccttcaggat actatttatt atgttcgtca
     4441 atgatatttt cacccgcgta aaacgttcag aaaataaaaa aatggcggaa atcgcccaat
     4501 tcctgcatga aaatgatttg agcgttgaca ccacagtcga agtatttatt accgtaaccc
     4561 gcgatgaaaa gcttatcgcg tgcggtggaa ttgccggaaa tattattaaa tgcgttgcta
     4621 tcagtgaatc cgttcgcggt gaaggactgg cgctgacatt agccactgaa ctgataaacc
     4681 tcgcctatga gcggcacagc acgcatctgt ttatttatac caaaaccgaa tacgaggcgc
     4741 tgttccgcca gtgcggtttt tccacgctga cctgcgtacc cggcgtgatg gtgctgatgg
     4801 aaaacagcgc cacgcgactg aaacgctatg ccgaatcgct gaaaaaattg cgtcatccag
     4861 ggaacaagat tggctgcatt gtgatgaacg ccaatccctt tacgaatggt caccgttatc
     4921 tgattcaaca agctgcggca cagtgcgact ggtttcatct gtttttagtc aaagaagatt
     4981 cttcacgctt cccctatgaa gaccggctgg atctggtgtt aaaaggcacc gccgatattc
     5041 cacgcctgac tgtgcatcgc ggctcagaat acatcatctc ccgcgctacg ttcccttgct
     5101 acttcattaa agaacagagc gtcattaacc attgttacac cgaaattgat ctgaagattt
     5161 tccgtcagta cctcgctccc gcgcttggtg taactcaccg ctttgtcggt actgaaccct
     5221 tttgtcgcgt taccgcccag tacaaccagg atatgcgcta ctggctggaa acgccgacta
     5281 tctccgcagc gcccatcgaa ctggttgaaa ttgagcggct gcgttaccag gagatgccga
     5341 tatccgcttc ccgggtacgt caactgctgg cgaaaaacga tctcacggct atcgcgccgc
     5401 tggtccctgc agtcacgctg cattatttgc agaacctgct tgaacactcc cgctaggacg
     5461 cggcagctcg tcaaaagacc cccgcatgag aaacaggtga aaaatgaaaa taaaccagcc
     5521 cgccgttgca ggcacccttg agtctgggga tgtgatgata cgcatcgccc cactcgatac
     5581 gcaggatatc gacctgcaaa tcaatagcag cgttgagaaa cagtttggcg atgcaattcg
     5641 caccaccatt ctggacgttc tcgcccgcta caacgtgcgc ggcgtacagc tgaatgtcga
     5701 tgacaaaggc gcactggact gcattttacg tgcacgactg gaagccctgc tggcacgcgc
     5761 cagcggtatc ccggctctgc catgggagga ttgccaatga tttccgcttc gctgcaacaa
     5821 cgtaaaactc gcacccgccg cagcatgttg tttgtgcctg gtgccaatgc cgcgatggtc
     5881 agcaactcct tcatctaccc ggctgatgcc ctgatgtttg acctcgaaga ctccgtggca
     5941 ttacgtgaaa aagacaccgc ccgccgcatg gtttatcacg cgctgcaaca tccgctgtat
     6001 cgcgatattg aaaccattgt gcgtgtcaac gcgctggatt ccgaatgggg tgttaacgac
     6061 ctggaagccg tcgttcgcgg tggtgcggac gttgtgcgtc tgccgaaaac cgataccgct
     6121 caggatgttc tggatattga aaaagagatc ctgcgtatag aaaaagcctg tggtcgtgaa
     6181 cccggcagca ccggcctgct ggcggcgatt gaatctccgc tgggcattac ccgcgcagtg
     6241 gaaatcgctc acgcttccga gcgtttgatc ggtatcgccc tcggtgcaga agactatgtg
     6301 cgcaacctgc gtacagaacg ctccccggaa ggaactgaac tgctgttcgc acgctgttcc
     6361 attttgcagg ccgcgcgctc tgcgggtatt caggcgttcg ataccgtcta ttccgacgct
     6421 aacaacgaag ccggattcct gcaagaagcc gcccacatca aacagctggg ctttgacggc
     6481 aaatcgctga tcaacccgcg tcagattgat ctgctgcaca acctctacgc accgacccag
     6541 aaagaagtgg atcacgcccg ccgcgtcgta gaagccgctg aagccgccgc tcgcgaaggc
     6601 ctcggcgtgg tttccctgaa cggcaagatg gtggacggtc cggttatcga tcgcgcccgt
     6661 ctggtgctct cccgtgcaga actttccggc atccgcgaag aataaggcaa tcaaaatgac
     6721 gcagaaaatt gaacaatctc aacgacaaga acgggtagcg gcctggaatc gtcgcactga
     6781 atgcgatctt gccgctttcc agaactcacc aaagcaaacc taccaagctg aaaaagcgcg
     6841 cgatcgcaaa ctgtgcgcca acctggaaga agcgattcgt cgctcaggtt tacaggacgg
     6901 catgacggtt tccttccatc acgctttccg tggcggtgac ctgaccgtca atatggtgat
     6961 ggacgtcatc gcgaagatgg gctttaaaaa cctgacccta gcgtccagct ccctgagtga
     7021 ttgccatgcg ccgctggtag aacacattcg ccagggcgtg gttacccgca tttatacctc
     7081 tggcctacgc ggtccactgg cggaagagat ctcccgtggt ctgctggcgg aaccggtgca
     7141 gatccactct cacggcggtc gtgtgcatct ggtacagagc ggcgaactga atatcgacgt
     7201 ggctttcctc ggcgtcccgt cctgtgatga attcggtaat gccaacggct acaccggtaa
     7261 agcctgctgc ggctccctcg gctatgcaat ggttgatgcc gacaacgcaa aacaggtcgt
     7321 gatgcttacc gaagaactgc tgccttatcc gcataatccg gcaagcattg agcaagatca
     7381 ggttgatttg atcgtcaaag ttgatcgcgt tggcgatgct gcaaaaatcg gcgctggtac
     7441 tacccgtatg accactaacc cgcgcgaact gcttattgcc cgtagcgctg cggatgtgat
     7501 tgtcaactct ggctacttca aagaaggttt ctccatgcaa accggcaccg gcggcgcttc
     7561 gctggcggta acccgtttcc tggaagacaa aatgcgtagc cgcgatattc gcgccgactt
     7621 cgccctcggc ggtattaccg cgacgatggt tgatctgcac gaaaaaggtc tgatccgcaa
     7681 actgctggat gtgcagagct ttgacagcca tgctgcgcaa tcgctggccc gtaaccccaa
     7741 tcacatcgaa atcagcgcca accagtacgc taactggggt tcgaaaggcg catcggttga
     7801 tcgtctcgac gtggtggtac tgagcgcgct ggaaattgac aggcagttca acgttaacgt
     7861 gctgaccggt tctgacggcg tactgcgtgg cgcttccggt ggtcactgcg ataccgcgat
     7921 tgcctctgcg ctttccatca tcgtcgcgcc actggtacgc ggtcgtattc cgactctggt
     7981 ggataacgtg ctgacctgca tcaccccggg ctccagtgtc gatattctgg tcacagacca
     8041 cggtatcgca gttaacccgg cacgtccgga actagcagaa cgtctgcagg aagcgggcat
     8101 taaagtggtt tccattgagt ggctgcgcga acgtgcgcgt ctgctgaccg gtgaaccaca
     8161 gccgattgaa ttcaccgacc gcgtcgttgc cgttgtgcgt taccgcgatg gctcggtgat
     8221 cgatgttgtg catcaggtga aggaataagc catgcacctg cttcctgaac tcgccagcca
     8281 ccatgcggtg tcaattcccg agctgctcgt cagccgggat gaaaggcaag cacggcaaca
     8341 cgcctggctc aagcaccatc ctgttccact ggtctccttt accgtggttg cgcctgggcc
     8401 gattaaagac agcgaggtca cacgccgaat ttttaatcat ggcgtgacag ccttgcgtgc
     8461 cttagccaca aagcagggct ggcaaattca ggagcaggct gcactggttt ccgccagcgg
     8521 gccggagggc atgttgagca ttgccgcccc ggctcgcgat ctcaagctcg ccaccattga
     8581 gcttgaacat agtcatcctc tcgggcggtt atgggatatc gatgtcctga cgcccgaagg
     8641 cgaaattctc tcccgccgcg actattcact gccgcctcgc cgctgcctgt tgtgcgagca
     8701 aagcgcagcc gtctgcgcgc gtggaaaaac ccatcaactg accgatttac tcaaccgcat
     8761 ggaggcactg ctgaacgatg tcgatgcctg caacgtcaac taaaaccaca aagcttgcga
     8821 cgtcattaat cgatgagtac gccctgctgg gctggcgcgc catgctgact gaagtcaatc
     8881 tgtcaccgaa accaggcctc gtggatcgca ttaactgcgg tgcgcacaaa gatatggcgc
     8941 tggaagattt ccaccgcagc gcgctggcga ttcagggctg gttaccccgt ttcattgaat
     9001 ttggtgcctg tagtgcagaa atggcaccgg aagcggtact ccacggatta cgcccaattg
     9061 gtatggcttg cgaaggtgat atgttccgcg ccactgcggg cgtaaacacg cataaaggca
     9121 gcattttttc tttagggctg ctatgtgcgg caattggccg tttgcttcag ctcaaccagc
     9181 cggttacgcc aacaaccgtt tgttctacgg cggcaagttt ctgccgtggc ctgaccgatc
     9241 gcgaactgcg taccaataat tcacgactga cggcaggtca acggttgtac cagcagcttg
     9301 gccttaccgg cgcacgcggt gaagccgaag cgggttatcc actggtgatc aaccacgcct
     9361 tgccgcatta cctcactctg ctggatcagg ggttagatcc tgaactggca ttgctcgata
     9421 ccttgctcct actgatggct accaacggcg ataccaacgt tgcatcgcgc ggtggcgagg
     9481 ggggcctgcg ctggctacag cgcgaggcgc aaacattatt gcaaaaaggg ggcattcgaa
     9541 cccccgccga tctcgattat ctccggcagt tcgacaggga gtgtatcgaa cgaaatctca
     9601 gtccaggcgg cagtgccgac ctgctgatcc ttacctggtt tttagcacag atttaattat
     9661 ttaagcactt gataaatttg gaaatattaa ttttcggaga acccgtatgt ctttagcaaa
     9721 agataatata tggaaactat tggccccact ggtggtgatg ggtgtcatgt ttcttatccc
     9781 tgtccccgac ggtatgccgc cgcaggcatg gcattacttc gctgtgtttg tggcaatgat
     9841 tgtcggcatg atcctcgagc caattccggc aacagcgatc agttttattg cggttactat
     9901 ttgcgttatt ggcagtaatt acctgctctt tgatgccaaa gaattagctg acccagcgtt
     9961 taatgcgcaa aaacaggcgc tgaaatgggg cctggctggt ttttccagca ccacggtatg
    10021 gctggtattt ggcgcattta tttttgcatt agggtatgaa gtttccgggt taggtcgtcg
    10081 cattgccctt ttcctggtga aattcatgga caaacgcacg ctgacgttgg gttatgcgat
    10141 tgtcattatc gacattctgc tggcaccgtt tacaccgtcc aacaccgcgc gtaccggggg
    10201 tacggttttt ccggtcatta aaaacctgcc gccgttgttt aaatcattcc cgaacgatcc
    10261 gtccgcgcgt cgtattggcg gctatttgat gtggatgatg gtcattagta ccagtctgag
    10321 ttcatccatg tttgtcaccg gtgcggcacc aaacatgctg ggtctggagt tcgtcagcaa
    10381 aattgccggt atccagatta gctggttgca gtggttcctc tgcttcctgc cggttggtgt
    10441 tatcttgctt atcattgcgc cgtggctttc ctacgtgctg tacaaaccgg aaatcacaca
    10501 cagtgaagaa gtggcaacct gggcgggtga tgaactaaaa accatgggtg cgctgacacg
    10561 cagagagtgg acgctgattg gccttgtatt gctcagctta ggtttgtggg tatttggcag
    10621 tgaagtcatt aatgctactg cggttggtct gctggcagtt tcgctaatgc tggctctgca
    10681 cgttgtgccg tggaaagaca ttacccgcta taacagcgca tggaacacgc tggtcaacct
    10741 ggcaactctg gttgtgatgg ctaacggcct gactcgttct ggttttattg actggttcgc
    10801 cggtaccatg agtacgcacc tggaaggatt ctcaccaaac gcaacggtga ttgtactggt
    10861 tctggtgttc tactttgcac actacctgtt tgccagcctg tctgcgcaca ccgcaaccat
    10921 gctgccggtt attctggccg tcggtaaagg tattccgggc gtaccaatgg aacaactgtg
    10981 tatcctgctg gtgctgtcta tcggtatcat gggctgtctg acgccgtatg caaccggtcc
    11041 tggggtgatt atttacggct gtggctatgt gaaatcaaaa gattactggc gtcttggcgc
    11101 aatcttcggg gtgatttaca tctctatgtt gctgttggtt ggctggccga ttctcgccat
    11161 gtggaactaa tacgttcgcc aaacgctgaa taaatcatca cgcccaccag gtaagcccac
    11221 ctggcgggct tttttatgat ttaatagata gtattcagtg tatgtatgag ttccacaccc
    11281 attatgaaag cattctgccg taacgccgcg ttgctcgcgg tttctctgct tcccttatct
    11341 tctgccaacg ccgtagcgtt gcaggcaaaa cagtatggcg attttgatcg ctatgtcctg
    11401 gccctctcct ggcaaaccgg attttgccag agtcaacacg atcgaaatcg taacgaacga
    11461 gatgaatgtc gcctgcaaac cgaaacgacc aacaaagctg attttctgac cgtacatggt
    11521 ctgtggccag gattgcctaa atcggttgct gcccgtggtg ttgatgaacg ccgctggatg
    11581 cgcttcggtt gcgctactcg cccaatcccg aatctaccag aagcacgcgc cagccgaatg
    11641 tgttcatcgc cggaaaccgg gttatcactg gaaacggccg ctaaactaag tgaagtcatg
    11701 ccaggagctg gcggacgttc ctgcctggaa cgctacgaat atgccaaaca cggtgcctgc
    11761 tttggttttg atccggacgc atacttcggt acgatggtac gcctgaatca agaaattaaa
    11821 gagagcgaag ccggaaaatt ccttgcggat aattacggta aaacagtgag ccgccgtgac
    11881 tttgacgccg cctttgccaa aagctgggga aaagagaacg tgaaagcagt taagctaacg
    11941 tgccagggta accctgcgta tttgactgaa attcagatct cgatcaaagc tgacgccatc
    12001 aacgctccgc tttctgcaaa ctcattcttg ccacaacctc acccaggtaa ctgtggcaaa
    12061 acctttgtga ttgataaagc gggttattaa tcctctgatt tacaaaacac gctaattcgt
    12121 cttccataac catgccgatt aagaattata cataatgcgc tgcattgagg aattccctga
    12181 atgtgacgca aatcacttca aagagtgagc aacagtcagt atcatcatgt gagttaaacc
    12241 ctcgccgcct gacggtgagg gttttctttt gggtatgttt atctgcgaca ctcgcagtac
    12301 cgacgacatg gagtaaaaat gtccagacca actatcatca ttaacgacct ggatgccgaa
    12361 cgcatcgata ttctgctgga gcaacccgcc tatgctggtt tgccaatcgc cgacgcgtta
    12421 aacgcagagt tggatcgcgc ccaaatgtgt tcgccagaag agatgccaca cgacgtggtg
    12481 acaatgaaca gccgggttaa attccgcaat cttagagatg gcgaagtgcg tgtgcgcacg
    12541 ctggtgtatc cggcaaaaat gaccgatagc aatactcagc tttccgttat ggctccggta
    12601 ggtgccgcac tgctggggct gcgcgttggc gattccattc actgggaact tccgggcggc
    12661 gttgcaaccc accttgaagt gctggaactc gaataccagc cagaagctgc tggcgactac
    12721 ctgctttaat cccatctgca caagcgataa ctcgctttgc ccagaggatg cactcgcatc
    12781 ctctttacgc aaaatcagca atttttcctg gttctcactg ttcacttttg ctgcaaaatt
    12841 attccgtttc gtttgtggga ataaccatgg cagaagcatt ttacatcctg ataggatttc
    12901 tgattatggc ggcgattatc gtcatggctg tcctttacct cgaaaaccac tcctgacgat
    12961 catattgttc cccccggcat cgcattcacc agacctgaaa ccgccttctg cgccgcctct
    13021 gcgctttgtg caccgggtac cagaatcacc ttacggcact cctcttcacg tttttcgaaa
    13081 atctcatatc cccgggcggc ctcttcaaac ggcatatagt gggtaacaat ttcttctggt
    13141 ttcagtaatc ctttctcaat taacggtaat aattctccca gccatgcgtg aacgtgggtc
    13201 tgtcccattt taaacgtcaa ccctttatca aaggcgtcgc caaacaggaa accgtgaata
    13261 aatccagcgt agacgcccgg tacgctaaca atgccgccac gcctgaccgc cgcaatacac
    13321 tggcgcaacg ctttaccgct gctgccctcc agtttcaggt tagtcagcac cgtttccgtg
    13381 gtgctgcctt tcgcttcaaa accgacggcg tctattactg catccacgcc ccggtgacct
    13441 gccgtttgtt caataattga ctgtgccgga tcgctatctt catcgaaatt aatcgggatc
    13501 gcgccgtagc ggtcagcggc gaaacgcaag cggtagggat gatgatcaac aacaaaaatc
    13561 tgttccgcac cgagcaaccg tgcacaggcg attgtcaaca atcccacagg accagcacca
    13621 tagactgcaa cgcttgaacc ttgttggatc tgcacatttt ttgctgcctg ccatgccgtt
    13681 ggcagaatat cagaaaggaa aagggcttta tcatctgaaa gcaaaggcgg tactttaaac
    13741 ggccccacat tccctttagg gacgcggaca tattccgcct gcccgccagg aacgccacca
    13801 tacaggtgac tataaccaaa caatgccgcg ggagcaggta tctgtttttt attgagcgca
    13861 gcgcctttac ccgcattggt attttcgcag gcggcatatt gctgtaatcg acagaaaaaa
    13921 cagtcgccac aagcaatgac gaacggaatt accacccggt cgcctttttg caaatttttt
    13981 acgtccttcc cggtttcaac gacttcccca taaattcatg accaaaaata tcgccatgtt
    14041 taacctgggg tattttgcct cgataaagat ggaggtcaga gccacagatc gccgttaccg
    14101 taatacgcag aataatatca tctgcctgtt caatgcccgg atcgggaaca ttttctacct
    14161 gaacgtgatg tgggccgtga taagtcaatg ctttcatgca acctccagag acagttcaaa
    14221 aaagagtcag tctttaaggg tagctatcac agcgggaaaa cgggcggtac cgcaggtttt
    14281 gggagaattc ctggtgttca gtgagaatat attaatcaca gaaaccgttc gtaaggaaaa
    14341 agaaccagac aaatatcagg gataaatatt ataatcatgg cgaatgattt gttcatgatt
    14401 aacagggaga aagggttatg tataaggcaa tcattatgcc agttgatgta tttgaaatgg
    14461 aattgagcga caaagctgtt cgccacgctg aattcctcgc ccaggatgac ggagttattc
    14521 atctacttca cgtactaccc gggtcagcca gcctgagcct gcaccgtttt gccgctgatg
    14581 tgcgtcgttt tgaagagcat ctgcaacatg aagcagaaga acgtctgcaa acgatggtca
    14641 gccacttcac catcgatcct tcccgcatta aacaacatgt ccgttttggt agcgtgcggg
    14701 atgaagtcaa tgagttggca gaagaactgg gggctgatgt tgtagttatc ggttctcgca
    14761 acccatcgat ttcgacccat ctgttaggtt ctaacgcctc gagcgtgatc cgccacgcca
    14821 atctgcctgt actggtcgtt cgctaacaga taaaagtatt tatgccgccc tgcctggaac
    14881 atcccggcag ggctttttaa tgccataaaa aagccgccag gtttgacccc ggcggctaag
    14941 caattgcagg tgaatcttac ttcttcttat gcagttttgg tgcgaatcag gtagtcaaaa
    15001 gcactcagag aggctttggc accttcgcca gtggcgatga tgatctgctt gtacggaacc
    15061 gtcgtacagt cacccgctgc gaacacgcct ttgacgttgg tttcacattt cgcatcaatg
    15121 ataatctcgc ccatgcggtt acgttcgact gcgccttcca gccagttggt gttcgggagc
    15181 agaccaatct ggacaaaaat accggccagt tcgatgttgt gaatattgcc gctgacacga
    15241 tctcgatatt ccagaccaac gactttgctg ccgtcgcctt tcacttccgt ggtttgcgca
    15301 ttcagaataa tgtcgacgtt tttcaggctg cgcagtttgt cctgcagaac ctggtcggct
    15361 ttcatttctg gcgcaaattc cagcagcgtt acgtgctcaa cgatacccgc caggtcgatt
    15421 gccgcttcca cgccggagtt accgccgccg ataaccgcta cgcgtttgcc tttaaacagc
    15481 gggccgtcgc agtgcgggca gtaggtcacg cctttagtgc gatactgatc ttcgcccgga
    15541 acgttcatgt tgcgccattt tgcacccgtc gccacgataa tgctgcgtgc tttcagtacc
    15601 gcgccagaag ctgtttcaat ctggtgtaga ccaccttcaa ccgctgccgg gatcagtttg
    15661 ctggcgctct ggctgtcgat cacatcaacg tcgtattcat caacgtgaac tttcagtgcg
    15721 cctgccaact tctgcccttc agtcttcggt acagaaatgt agttttcgat atcaacggta
    15781 tcgaggatct gaccaccaaa acgttcgccc atcagaccgg tacggatgcc tttacgtgcg
    15841 gagtaaattg ctgccgctgc acccgccggg ccggaaccaa cgattaatac gtcataagca
    15901 tcacgcttgt tcagctcttc tgccgcacgt ttttccgcgc cagtatcaat tttggcaacg
    15961 atttcagtca acgtcatgcg gccctgacca aactctttcc cgtttacgaa cactgccgga
    16021 acgcccatca cgttgcgatc ggtgatttcg ttctggaagg tgccgccgtc aattgcagtg
    16081 tgcttgatgc gcgggttcaa tacgctcatc aggttcagcg cctgcaccac gtccgggcag
    16141 ttatggcaag agagcgagta ataggtttcg aattcaaaat caccgtcaat atggcgaatc
    16201 tgctccagca gagactgcgc ttctttcgac ggatgaccac cggtccacag caacgccagt
    16261 accagcgagg tgaactcgtg gcccagcggg gagcctgcaa aacgtggtcc ctggttggaa
    16321 cctgggttgg tgatcaggaa agacggctta cgcactggca agctgttatc ttctttaaag
    16381 gtgactttgt ctgacagttc tgcgatttca gccaacagtt ccttgatttc tgccgattta
    16441 gcgctgtcat ccagcgtggc aattaactca acaggcttgg tcaatttctc aaggtaagcc
    16501 ttgagttgag ttttcatatt tgtgtcgagc atgtttatct cctgggcttt aaacatcatc
    16561 atgcaagcgg cctctgcggc tacctggatg cagcttgcat catggtgcgg ggaagaaaat
    16621 cgggtgccgc agcacccgaa gaattaggtg aatttccgaa gtatttcgcg tcagagcaag
    16681 gcgactagcc tgtgaatccc cgggagctta cacaagtaag tgaccggggt gagcgggcga
    16741 cgccaacgcc gctatggcgt gaaagacgaa ggaaatttag attttaccaa ccaggtccag
    16801 agacggagcc agagttgctt caccttcttt ccatttagcc gggcaaactt cacctgggtg
    16861 agaagctacg tactgagctg ctttgatttt acgcagcaga tcagacgcgt cacggccaat
    16921 gccttcagcg gtaacttcga ttgcctggat gataccctgc gggtcaacaa cgaaggtcgc
    16981 acggtcagcc agaccttcat cttcacgcat gttgtcgaag ttacgggtca gggcgccagt
    17041 cgggtcgccg atcatcgcat atttgatttt agcgatggtt tcagagctgc tgtgccatgc
    17101 tttgtgggtg aagtgagtat cggtagatac tgcgtatacg tctacgccca gtttctgcag
    17161 ttcttcgtag tggtcagcaa cgtcacccag ttcggtcggg catacgaaag taaagtcagc
    17221 cgggtagaag aagaagacgc tccagcggcc ttcggtatct ttttcggtga tttcgatgaa
    17281 ttcgccgttt ttgaatgcct ggtttttaaa aggtttaatt ttggtgttaa tcaaggacat
    17341 ctatacttcc tccgtgtttt cgatgagatg taaggtaacc aatttttgcc gattcggcta
    17401 atgcgtttcc attatcaaat cgataagttt taccttacaa ccttcgtaag acaactttgt
    17461 gaactttgca ccgccagcgg cgataaaaag taaaatggcc ccctttccag gtgaccgtgt
    17521 aatctgaact accctttggt atcttcagcg cctgcctgca ggctgaggtg ttgcccttac
    17581 aaatgcaaca acgacatgga ttacaacacc ctcataaaca aagggcaatc acctgatcta
    17641 agctcttacc tatgacagtg ataggttatg ccttttactc gacttttgca ctgactgaaa
    17701 agaacaaatt aatgttaaaa aagatacttt tactggctct gcttcctgca atcgccttcg
    17761 cagaggaact tccttcccca gtaaaagcga ttgaaaaaca gggcattaca atcatcaaaa
    17821 cgttcgatgc ccccggagga atgaaaggtt atctcggaaa gtatcaggat atgggcgtca
    17881 ccatctacct gactcccgat ggtaagcacg ctatctctgg ttacatgtac aacgagaaag
    17941 gtgaaaacct gagtaacaca cttatcgaaa aagaaattta cgcaccagcc ggacgcgaaa
    18001 tgtggcaacg gatggaacaa tcccactggc tcctcgacgg taaaaaagat gcgccggtca
    18061 ttgtctacgt cttcgccgat ccgttctgcc catattgtaa acagttctgg cagcaggcgc
    18121 gcccgtgggt agattctggc aaagtgcaat taagaacatt gttggttggg gttatcaagc
    18181 cagaaagccc ggcgacagca gcggcaattc ttgcctccaa agatcccgca aaaacctggc
    18241 aacaatatga agcctctggt tgcaagctta agctaaacgt gcctgcaaac gtaagtacag
    18301 agcaaatgaa agtgttaagt gacaatgaga aactgatgga cgatctgggg gcaaatgtca
    18361 cgccggctat ctattacatg agtaaggaaa atacgctgca acaggccgtg gggttgcccg
    18421 atcagaaaac gcttaatatc attatgggaa ataaataaaa ggtaattcat ggatggttga
    18481 actatatccc tgcttttcaa ccatcctaaa aaagcatgaa ttgctcgctc taccctaagt
    18541 ggctgtgcta ttttagaaaa acttaatcct gagttaagca aaaaaacata atcaataaaa
    18601 atacatacgc aaaaaacatt tattaagtga atataccatg gaagaaaaat ataaaccgga
    18661 gtagtgtatg gccaatctct acgacttgaa aaagttcgac ttaaatcttc ttgtcatatt
    18721 tgagtgtatt taccagcatt taagcattag caaagctgct gaatcactat atatcacccc
    18781 atcagccgtg agccagtcgt tacaacgctt acgtgcgcaa tttaatgacc ctttattcat
    18841 tcgctcagga aaaggtatcg cgccgacgac aacagggttg aatctgcatc accatcttga
    18901 gaaaaacctc aaaggccttg aacaaacaat taatatagtc aataagtccg aactaaagaa
    18961 aaactttatt atctacggcc ctcagctcat ttcttgctct aataatagta tgctgatccg
    19021 ctgcctacgg caagatgcct ctgtcgagat tgagtgccat gatatcctca tgtcagcaga
    19081 gaatgctgaa gaactgttgg ttcaccgcaa agccgatctg gtgatcaccc aaatgccagt
    19141 gatcagccgt tccgtcattt gcatgccatt gcatacaatt cgcaatactc taatttgtag
    19201 taataagcat ccgcgaatta ctgacaatag cacttatgaa caaattatgg cagaagagtt
    19261 cacccaatta atatccaaat cagcaggagt tgatgatatt caaatggaaa tagacgagaa
    19321 gtttatgaat cgcaaaattt catttcgtgg ttcctctttg ctgacaataa ttaatagcat
    19381 tgcagttaca gatttactgg gaatagtccc ctatgaactt tataactacc accgtgactt
    19441 tcttaattta aaagagataa aactagaaca tccgttacct tcaataaagc tttacatttc
    19501 ctataataaa tcttctttaa ataatttggt gttctcccga tttattgacc gtctaaatga
    19561 gagtttttga tatataacta cagacagcta ctataacttt catctattta ttcacagctc
    19621 gaatgaaaaa taaaacactc tgctttacag tgtttacttt ataattaaca gtcttatctc
    19681 actgttcacc ctgtaacgtt cagagagcgt cttcagatgt ctatttataa aattcctctt
    19741 ccactcaata ttctggaagc tgcacgagag cgtattacct agacactcaa caccctaccc
    19801 cgcgtttgtg tctcattttc tggcggcaaa gactctggtt taatgctgca tctgacagcc
    19861 gaacttgccc gacaaatggg caaaaaaatc tgcgttttgt ttatcgactg ggaggcgc
//
LOCUS       DAJTYG010000070        19901 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTYG010000070 DAJTYG010000000
VERSION     DAJTYG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.070"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..376
                     /locus_tag="OO568_002691"
     CDS             <1..376
                     /locus_tag="OO568_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HCR6641992.1"
                     /translation="GAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMS
                     ERPIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPIRCAFTGALTLYYYFIG
                     FFGILAAIAITLVWQRHTRFFH"
     gene            complement(412..1368)
                     /locus_tag="OO568_002692"
     CDS             complement(412..1368)
                     /locus_tag="OO568_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR6641993.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IITALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1368..2609)
                     /gene="clsB"
                     /locus_tag="OO568_002693"
     CDS             complement(1368..2609)
                     /gene="clsB"
                     /locus_tag="OO568_002693"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6641994.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQKRIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQCEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2606..3367)
                     /locus_tag="OO568_002694"
     CDS             complement(2606..3367)
                     /locus_tag="OO568_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6641995.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3500..3910
                     /locus_tag="OO568_002695"
     CDS             3500..3910
                     /locus_tag="OO568_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6641996.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3872..4978)
                     /locus_tag="OO568_002696"
     CDS             complement(3872..4978)
                     /locus_tag="OO568_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6641997.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4989..6122)
                     /locus_tag="OO568_002697"
     CDS             complement(4989..6122)
                     /locus_tag="OO568_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6641998.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6115..7851)
                     /locus_tag="OO568_002698"
     CDS             complement(6115..7851)
                     /locus_tag="OO568_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6641999.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7844..8839)
                     /gene="hlyD"
                     /locus_tag="OO568_002699"
     CDS             complement(7844..8839)
                     /gene="hlyD"
                     /locus_tag="OO568_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6642000.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(8842..9513)
                     /gene="cecR"
                     /locus_tag="OO568_002700"
     CDS             complement(8842..9513)
                     /gene="cecR"
                     /locus_tag="OO568_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6642001.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEETERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHSHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9742..10929
                     /gene="rhlE"
                     /locus_tag="OO568_002701"
                     /pseudo
     CDS             9742..10929
                     /gene="rhlE"
                     /locus_tag="OO568_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(11136..11618)
                     /gene="ybiA"
                     /locus_tag="OO568_002702"
                     /pseudo
     CDS             complement(11136..11618)
                     /gene="ybiA"
                     /locus_tag="OO568_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
     gene            11738..13888
                     /gene="dinG"
                     /locus_tag="OO568_002703"
     CDS             11738..13888
                     /gene="dinG"
                     /locus_tag="OO568_002703"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6642002.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            13916..14878
                     /gene="ybiB"
                     /locus_tag="OO568_002704"
     CDS             13916..14878
                     /gene="ybiB"
                     /locus_tag="OO568_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6642003.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEVATISDGLARVNQAF"
     gene            15019..16104
                     /gene="hcxB"
                     /locus_tag="OO568_002705"
     CDS             15019..16104
                     /gene="hcxB"
                     /locus_tag="OO568_002705"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6642004.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(16333..16593)
                     /locus_tag="OO568_002706"
     CDS             complement(16333..16593)
                     /locus_tag="OO568_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6642005.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(16885..17124)
                     /locus_tag="OO568_002707"
     CDS             complement(16885..17124)
                     /locus_tag="OO568_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HCR6642006.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNR"
     gene            complement(17198..17875)
                     /gene="ybiX"
                     /locus_tag="OO568_002708"
     CDS             complement(17198..17875)
                     /gene="ybiX"
                     /locus_tag="OO568_002708"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6642007.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(17917..19901)
                     /locus_tag="OO568_002709"
                     /pseudo
     CDS             complement(17917..19901)
                     /locus_tag="OO568_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4616 a         5224 c         5374 g         4687 t
ORIGIN      
        1 gggcgcgatg tttttaatca gtatgctggc cggtttatta tttaatgttg atcctggttc
       61 tcaccgtttt atcattatga tgacgttgac agggttggcc ctggtaatca tcgtgaatgc
      121 ggcattaatg agtgaacggc ccatttgggt aataagttgc ttaatgattg tgttatggcc
      181 aggcattatc tcgcatggac gaaataagct ccttgaactg gcaggaaaat gccatagtga
      241 agagttgtgg agtccgattc gttgcgcttt tacaggtgca ttaacactct attactattt
      301 tatcggcttc tttgggatac ttgccgcgat agctataacg cttgtctggc aaaggcatac
      361 gcgttttttt cattagtcgt aaatgcagat actaaagaac ttaactaaac ttcacatcgc
      421 cgcttcattt ttcgcccgta gcttcttcgc ttggctttcc agtagcaggt aacagatcag
      481 cgccagcagc agcgggataa agtaatacag gacgcgataa gcgagcaggg cggtgataat
      541 gctccctttc gaggtatgct ccccagccag tagtgcgata aacaccgctt ccagcacacc
      601 gatccccgcc ggaatatgca cgatgacgcc agcaatacta ctaaccagta acacgcccag
      661 tacaaagaaa tagttcacgc tttgaccaag taacagccag ataatcgccc ccattaccat
      721 ccagttaacg ctggaaatca gcatctgagc gagggcgaat ttccatgaag gcagcaccag
      781 cttttgccct ttgatggtca tatggcggtg cttcgcgaaa gcgcaaaacc acaaataaac
      841 cgcgataatc atcagtaagc caatgccgag aatacgcagc gtagtttgat cgacatacca
      901 gtgatccggc aactccacca cgcctgcggt aaagataatc cctgccagta aaatatagcc
      961 cagccagttg gtggtaatac tgagcgagga aatccgcgta atagtgctgc ccggtaaccc
     1021 tagtcgggag tacaaacgat agcgcatacc aatgccgccg acccaggtac tgagcgtcag
     1081 gttgaaggcg tagcagataa acgacaccag catcacctgg cgcttcgcca gtttgtgacc
     1141 gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc agatagctga cgaccaccag
     1201 cccgaccgca ctaagcagcg caacgcgatt gtagtcgcgg atgaccttcc agacctcttc
     1261 ccagtccact tttttggcgt agaccaccag caacacaatc accgcgataa aaaacagcca
     1321 ggtgaggatc ttctttgcta agcgccagcg cgggtgtgat ttactcatca gggttttacc
     1381 cccgtgtttt cagtttctac ccgatcctgc gtttccattg tcggttgtgc ggggggatca
     1441 acctgcgtca gacgtggtgt gtgtgccgga agccagccaa ccagcgccgg gaagtggcgt
     1501 aaaaagtgga acgccagcac gcttttggtc aggttccacc aggtgcgttt gggcagcatg
     1561 gtttcatcca cctgctgaca atctgcggca ataatgccgt tcagattatc gcgcagcgtc
     1621 tggttaaaat gacgatcgtg gatgatgaca tttgcttcga gattcagtga caaactgagc
     1681 ggatcgagat tactggaccc tactgtcgcc cagtgatcgt ccatcaatgc cactttgccg
     1741 tggagcgggc ggcggcggta ctcaaaaacc tgaacgccgc ctttaaccag atagttatac
     1801 agcaagcgcg caccgactct gacaatcggc atatccggtt cgcactgaat gatcagtttg
     1861 atccgcaccc cgcgccgtgc cgctttacgc aaggcgtgta aaaatcgata gccggggaag
     1921 aagtaggcgt tggcgataat cacttcccgc cgcgcctgag tgagcatttt cagataatgg
     1981 cgttcaatat catcgcgatg ttcttcgtta tcgcgccaga ccagcaatac ctgcgcttct
     2041 cccggctggc ggttctcttc cgctttgtga tgacgtcgcc accagcgtcg tgccgcgctc
     2101 tgtccaggca ggttttccag ctcaaactgg agaatatctt cgacaatcgg cccttcaagg
     2161 cgtaccgcgt aatcctgttt agcctctgga ccgtagctgg acatatgctc ggcggagtaa
     2221 ttcagcccgc caataaaggc gatacgcgcg tcgatcacca caattttgcg atgcatccgg
     2281 cgaaacacat tggtgcgcat accaaaaagg cgagggcggg gatcgtagta gcggaacact
     2341 acgccagctg ccgtcagttc attgacaaac tcatcgctga gatccggcga accgtagcca
     2401 tccagcaaga cttccgcttt aaccccgcgt tgcgctgctg ccagtagtgc cgcatgcagc
     2461 tgtttgccga cgttatcctc aaaccagata aacgtttcaa gaatgatgcg tttttgtgcc
     2521 tcgccaatcg ccttaaacac cgcgggataa tattgctcgc cgttttccag caactggatc
     2581 ttattgcctt cgcgccagct acatttcata aatgaatctc cgcacttaaa ggggcatgat
     2641 cagaaaggtg tcgccatgtc cgcagcggca acgcggttgg cgcgctggcg ctggcatttt
     2701 tgacgtagat cctgtccagt cgtagtagag gaaattgcac cggaaacgtg cgcgccgggc
     2761 gtccgtgggc gcgggtaaaa atctcatcca gtccggcctg cacttttaac ggatgattag
     2821 ctttttgccg ccagtcattg aaatcacccg ccaccaatac cggttcgccg tccggtagct
     2881 cattcaccca ttcggcgagc atcgcaagct gcgcctgacg gtgcgcctca cgcaggccca
     2941 gatgtacgca catcacatga atcgcttttc cggtcatcgg cggcacaatg cggcagtaga
     3001 gcacgccgcg cttttccgca ccatcgaccg aaacatcgcg attctcataa tgttcaatgg
     3061 gataacgcga cagtacggcg ttgccgtgat gcccttccgg gtatacggca ttgcgaccgt
     3121 aggcaaaatc gctccacata gtgtcggcga gaaactcgta gtgcgaggta tcgggccagt
     3181 tttccacatg cagcggatga acttcgtgcg cgcccatcac ttcctgcagg caaacaatat
     3241 cggcgctgac ggtacgcacg gcatcgcgaa gttccggcaa aatgaagcgt cggttaaacg
     3301 cggtaaagcc tttgtgaatg ttgatggtga gcaccttgaa cgaaaattgt tgtgtttgat
     3361 cgggcataat tttcctgtct ttgcctctta tctcattgaa atagtgtagt cggcgtcaca
     3421 aaaaggtgcg gtcttacgga attttccgta aagttcggta ctctgagtaa gtagagataa
     3481 attcttcagg agagaagcca tgaagtggca acaacgtgtt cgtgtcgcaa cgggtctaag
     3541 ttgctggcag attatgttgc atttactggt agtggcgctg ctggtggtgg gctggatgag
     3601 taagactctg gttcacgtcg gcgtgggatt atgcgcactg tattgtgtca cggtagtgat
     3661 gatgctggtg tttcagcgcc accccgagca acgctggcgt gaggtggcag acgtgctgga
     3721 agagctgacc acgacctggt attttggcgc agcgctgatt gtgctgtggc tgttgtcccg
     3781 cgtactggaa aacaactttt tgctggcaat tgcagggctg gcgatccttg ctggcccggc
     3841 ggtagtgtct ttgctggcga aagataagaa gttacatcac cttacgtcta aacatcgcgt
     3901 acgccgctga ccctgtcgtg gccgttatca ccagtagcgg ccacaaacta ttccacacaa
     3961 tatccagact cgcatccttc aaataaatct gcttggtaat gtccgtaaag tggcgaatag
     4021 ggttaatcca cgtcagattt tgcagccaca ctggcatgtt ttccaccggc gaaacgtaac
     4081 cggaaaggag aatggcgggc atcataaaaa caaacacgcc gataaacgcc tgctgttgtg
     4141 tcgagcagag tgatgaaatc aacagaccga atcccaccag cgataaaccg taaatcacca
     4201 tcgtaaagta gaacagcgct agcgatccgg cgaaggggat ttgatacgcc cagataccaa
     4261 tcgccagcac aatggtggcc tggaaggtgg cgacaattaa cgccggtacg gctttgccga
     4321 tgaagatctg ccaggtggtg agcggcgaaa ccagtagctg atcgagcgta ccttgttcac
     4381 gttcgcgggc gacggaaagt gaagtgacga tcattacgcc gatagtggtg atcatggcga
     4441 tcagcgacgg caccacaaac catttgtagt cgagattcgg gttataccag ttgcgtacca
     4501 ccagctcgct gttgttgggt ttcggttttc cttccagcag ctcctgctga taatttttga
     4561 cgatctgttg caggtagttg gcggcaattt gcgcactgtt ggagttacgc ccgtcgagga
     4621 tcaactgcaa cggcgcggtc tggaaggtat ccagtttgcg cgagaagtca gccgggaaac
     4681 gcaccagtag taacgccttt tgtgtgtcga tggtcgggcg gatctcctgt gggcttttca
     4741 gcagcagcac atgtgtaaag gcgctggcgc gggcaaaacg ttgagtaagt tccaccgaat
     4801 gctcgccgtt atcttcatcg tagatggcga tggtggcgtt agtcacttcc agcgtggcgg
     4861 cgaacgggaa caggatcacc tgaattagca cgggtaaaat cagaatcgcg cgggtttgtg
     4921 gttcgcgcag caacgactgc aactctttgc ggattaacgt ccataagcga tgaaacatgc
     4981 tcttctccct aatccagccg acgtttggtt ttcagccacg tcaggccgat aaacatcacc
     5041 gccgaagcga tcaaaaacag cacgtttacc accagcacca ctggaatatt cccggcgagg
     5101 aacaggcttt gcagggtgct gacgaaataa cgagcgggaa taatgtacgt caccgcgcgg
     5161 atcaccgcgg gcatactgtc gatctgaaaa ataaagccgg aaagcataat cgacggcaga
     5221 aaagcggcgt tcagggcgac ctgagcggca ttgaactggt tgcgggtaat cgtggaaatc
     5281 agcagcccca ttcccagggt actgagtaaa aacaggctgg agataaaaaa cagaatcagc
     5341 agcgaaccgc gatacggcac gccgagaata aacactgaca ccagcataca cagcaacatc
     5401 gccagcatcc cgagaaagta ataagggatc agcttacaca gcagcagttc cgtgcgggta
     5461 atctccgtag agagcagagc ctccatggtg ccgcgttccc attctcgcgc caccaccagc
     5521 gaggtgagaa tcgcgccgat gaccgtcatg ataatggtca ccgcacccgg gataataaag
     5581 tgctggctta tcgccgccgg gttaaaccag tagcgggttt gtacatcaat aagcggttca
     5641 aaagtctgcc cgttgtcctc cgctcgctgc atttgccaga tctgccagat cccttcgaca
     5701 tacccctgca caaagttagc ggtattcggc tcactgccgt cggtgatcac ctgaatcggt
     5761 gcggtggcgt tggcgcgctc catctgttcg gcgaaatcca cgggaataac caccagaccg
     5821 cgaattttcc ccgactgcat tttggcgatc agttcctgac ggttatcgct gatggtggcg
     5881 tcgatgtagg gcgaaccggt catggtgtgg gtgaaatcca gcgccgcttc gctacgctgt
     5941 tccagcaaaa tcccgacccg cagcttgctg gagtcgaggt taatgccgta accaaaaata
     6001 aacagcagta gcagtgggat cactaccgca atcagccagc tactcggatc gcgaacgatc
     6061 tgccgcgtct ctttgacgca cagcgcccgc acgcgatgcc aggaaaggat cgggttactc
     6121 attgctatgc tccttatccc agtcgtggat caactgaata aaggcttgct ccatggtggg
     6181 atcgggttgc tcatcgttag ctgactgtgc tttcaaatcg tccggcgtac cgctggcgat
     6241 taatttccca cggtacacca ggccgatgcg gtcgcaatat tccgcttcat ccataaagtg
     6301 ggtggtgacc atcaccgtga cgcctttctc taccatgctg ttgatgtgca gccaaaattc
     6361 acggcgggtg agggggtcaa cgccggaagt cggttcgtcg agaaagagaa tatccggttc
     6421 atgcatcagc gaacaggcca gcgccagccg ctgtttaaaa cctaatggca gttcatcggt
     6481 ggcgtgtgag gcaatacttt tcaggccaaa tgcctcgctc atacgggaga ttttttcgtt
     6541 ctgcgcccga ccgcgtaagc catacacacc agagaaaaag cgtaaattct gttcgaccgt
     6601 caggttgccg tagagcgaaa atttttgcgc catatagccg agatgctggc gcgctttacc
     6661 ggaactctct ttcagatcca tccccagcac cagcgcctgg ccggaagtcg gcaccagcaa
     6721 accgcacatc atcttaaagg tggtcgattt acccgcgccg tttggcccca gcaaaccaaa
     6781 aatctcccca cgtttaacgg caaagttgac gtgatcggtg gcggcaaaat caccaaattt
     6841 cttggtcagt tctttcgctt cgatcaccgt ctcgccgggt gtgccttcta ccgtatgcaa
     6901 tattgcaccc agcggcgatt ctgaggttcc ggcaccgccc agcaaatcaa taaacgcatc
     6961 ttcaaaacgc ggcgtagttt cgttgatgtt gatttccggc atcccgtcgg catggcgaat
     7021 atcgtctggt gtggcctctt ttttgaggat cagacgtacc gatttcccct gaatcatgcc
     7081 gtcgctgacc tgcggcagtt tcaaggcgcg ttgcaacagt ttgcggttgc cctcgtgtgg
     7141 actggtcatc agaaagctgc gtccggccat ggtttgtgtc agggcttttg gttctccctg
     7201 atacagcaac tcgccttcgt tcatcagtaa cacgtcacgg cactgctcgg cttcgtcgag
     7261 atacgaggta ctccagagga ttaacatccc ttcgcccgcc agctcatgca ccatctgcca
     7321 cagttcgcgc cgtgagatag ggtcaacgcc gacgccgggt tcatcgagca gcaacacttt
     7381 cggttcgccc accagggtac aggccaggcc gagtttttgt ttcatcccac cggagagctt
     7441 gcccgccagg cgtccggtaa acggcccaag agacgtaaac tccagcaggc gagcaaaagt
     7501 ttgcttacgt gcctcgccgg tgacgctgcg caaatccgcg tacagattga ggttctccat
     7561 caccgtgaga tcttcataca gaccaaattt ctgcggcata tagccgagca cggcgtgcag
     7621 cgcgccgtcg tttttgatcg gatcaaagcc aatcaccgtg gcactgccgc tgtcgggttt
     7681 cagtaatccc gccaacatcc gcatcagcgt ggttttacct gcaccgtccg gccccaccaa
     7741 ccccgtcaca taaccggcgt gaatggtaca atcgagcggc gcgacggcgg gcttgtccat
     7801 gcccggaaag cgtttttcca ggccgttcag cgtgataacg gcatcattca tgtcctgcct
     7861 cgtcaccgaa ttgtaccgtc actggcattc cctggcgtaa cgcatcatcg gcgtcggtca
     7921 ccacaatacg caggcgatag acgaggtcgg tacgcagatc cggtgtttcg acggttttcg
     7981 gggtaaattc agcagtcggc gaaacgaaac caatctgccc gtgatacggc ttgtccgggc
     8041 gaccatcggt ataaagcagc actttgcgcc ccggctgggc ctggtcaaga ttccgttcat
     8101 caacataagc gcgcacccac accggacgcg ttagtgaaac ggtaaacacc gtgccacctt
     8161 cattgagaac cgtgcctggc tctaccgcgc gcgttaacag cgtgccatca gacggtgcta
     8221 tcaacgttga gtcctgtaaa ttcagctccg cctgcgccag ttgcgcctgc gcctgttcga
     8281 ggctggcttt cgcctgagcg atatcctgtt cacggttacc ggaacggtac tgacgcaatt
     8341 tatcctgtgc tgatttcagc gttgcctgtg cctggtcgcg cgaggagcgg gcattttcca
     8401 ggtcatttgc cgaaatggtg cggcttttcc acagtccttg ctggcggtta tagaagttct
     8461 gcgcatagtc ataggcggct tgcgcctgtt tcaccgctgc ggcggcctga gcgatttctt
     8521 catcgcgata cccggcaagc atcagatcat actgcgcctg tgccaccgag acgcccgctt
     8581 tcgcctgcat cagggcaatc tcatacggct tgtgatccag ttcgcccagc acctggcccg
     8641 ctttgatagc atcaccttcg tccaccgcca gcgattcaac gcgcccccca acacggaaac
     8701 taagattcac cgtacgaata tccacgttgc catacagcgt caggccgtta tcctggcggc
     8761 tttgatacca ccagtagcct ccggcaacca cggcggcaag taccactacc gccagtccga
     8821 tcacgacagg ttttttcatc actacaaact cctttgcgat aatccttgca aaatcaggtc
     8881 gatatgacag gtcaccgtct ggttgatcag ttcggtcttt tcttcatcga acgcggtcca
     8941 gccggtacgt aacagaattg tttctttgcc aagacggaac gccagaatct caccaatcaa
     9001 cgcatgggaa tgaaggatca tgcgggtgtc attagcgtcg cagccggtcc aggcggcaat
     9061 caggcgtgtc aggtggctgt gtagcggact aatcacctgt tcgtgcacca ggtggtaggc
     9121 tgccgtggga gagagctgct cacgggagat aaacttgctg aggttgacgg tgtcatcctg
     9181 ggtgagcagc ttaatcatgt tcctgcaggc gcgaaggatc agttcacgga tggcagcccg
     9241 atcaggctgt ggttgggcga acaagcgttc ggtttcctcg gcatgcggac ggaactgctc
     9301 gccaataaaa tcggcaatcc actgggcgca ggcgaggtac aaatcttctt tcgaaccgaa
     9361 gtagtaggtg atggcagcga tattctgccc ggcctgggcg gctatctcgc gggtggtggc
     9421 gttcattcca tattcaccaa actgcgccag tgcggcagca atcagctgtt ttttcgcctg
     9481 ttcacccttg attgtcatgg caggattatt catcgcacag tccattctta atcaaatgat
     9541 tgattaagat tatgactcca tatgggagtt gtccagtatg gctaagaatt ttagctacgc
     9601 cagtcacagg gataatttat gcgctgcgtc acaaaaactg ctacactccg ctccctcatg
     9661 acattgtggt ttttgtcatt ttccttttca gtatctccct gaaaactaca ccggtaacgg
     9721 tcggggcggt tcggagtagt tatgtctttc gattctttgg gtttaagccc tgatatcctg
     9781 cgcgccgttg ccgagcaggg ttaccgtgaa cccaccccta ttcagcagca ggcgatccct
     9841 gcggtgctgg aaggccgcga cctgatggct agcgcccaga ccggcaccgg caaaacagcg
     9901 ggctttacgc tgccgctgtt gcaacacctg atcactcgcc agccgcacgc caaagggcgt
     9961 cgtccggtac gtgcgctcat tcttaccccg acccgtgaac tggcggcaca gattggcgaa
    10021 aatgtccgtg attacagcaa atacctgaac attcgttcgc tggtggtgtt tggcggtgtc
    10081 agcattaacc cacaaatgat gaagttgcgc ggcggcgttg atgtgctggt ggcaacgccg
    10141 ggacgtttgc tggacctgga acatcaaaat gcagtgaagc tggatcaggt tgaaatcctc
    10201 gtcctcgatg aagctgaccg catgctcgac atgggcttta tccacgatat ccgtcgcgtg
    10261 ttaacaaaac tgccagcgaa acgccagaac ctgctgttct ctgcgacctt ctctgacgat
    10321 attaaagccc tggcggaaaa actgttgcac aacccgctgg aaatcgaagt ggcacgccgc
    10381 aataccgcgt ctgatcaggt gacccagcac gttcactttg tcgataagaa acgcaaacgc
    10441 gaattgctgt cgcacatgat tggtaaaggg aactggcagc aggtgctggt gtttacccgt
    10501 accaaacacg gcgctaacca tctggctgag cagctcaata aagatggcat ccgtagtgtg
    10561 gcgatccacg ggaataaatc gcaaggtgcg cgtacccgtg cgctggctga ttttaaatcg
    10621 ggtgacattc gcgtactggt ggcgaccgat atcgctgcgc gcggtctgga tatcgaagag
    10681 ttgccgcacg tcgtcaacta tgaactgcca aacgtaccgg aagattatgt ccaccgtatc
    10741 gggcgtaccg gtcgtgcggc tgctaccggt gaagcgttgt cgctggtgtg tgttgatgaa
    10801 cacaaactgc tgcgtgatat cgaaaaactg ctgaaaaaag agatcccgcg cattgcgatt
    10861 ccgggctatg agccggaccc gtcaatcaaa gccgaaccga tccagaacgg tcgccagcaa
    10921 cgtggcggca tcaggctgat gaacaaacgc aaaactgcct gatgcgctac gcttatcagg
    10981 cctacgtgaa ctctgcaata tattgaattt gcatgctttt gtaggccgga taaggcgttt
    11041 acgccgcatc cggcatttca caacaagcac ttgtcagcaa tttgagaaca cgggaaaaaa
    11101 tttatctgtt ttaccgccca taagccaccg agacgttact tctctatagc caattgttcg
    11161 cgcaactcca ttaaaaggta gcccagtcga ttcttgccct taccattacc accgtctccc
    11221 cagtaagcat cgttttccgt atgctcaacc agttttgcgg gcgcggttgc cagcaagagc
    11281 acacgcagtt ctgcatgctg ttcgaattta gcgcgaagtg ctttacgcat cacctgttct
    11341 ttaaccgact cccagttttt acgaagtggc ttggaacgat tacgccccat gcgagcggcg
    11401 accatggggg aagaaacccg acggatctct tcgcggtatt tttcatcaag gaatttttgt
    11461 gcctgaaaat agtgttcgga ggtgggctag gtattcccgt caaccttgat gggcctggcg
    11521 gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt agaaatttat gatggtgtct
    11581 tgcatgacgt gttggatcct ttgtgctcga acgggcatta aaccgcatta tgttggtggt
    11641 tattgcgagc cgctttccag aaacagaaaa accattaccc ctgaaaaccg aaaaatgcca
    11701 caatattggc tgtttataca gtatttcagg ttttctcatg gcattaaccg ccgcgcttaa
    11761 agcgcaaatt gccgcctggt ataaggcgct tcaggaacag atccccgact ttattccccg
    11821 tgcgccgcag cggcagatga ttgcggacgt cgccaaaacg ctggccggag aagaagggcg
    11881 gcatctggcg attgaagccc ccaccggcgt tgggaaaacg ctctcctatt tgatccccgg
    11941 catcgccatt gcccgcgaag agcaaaaaac gctggtggtg agtaccgcca acgtggcgtt
    12001 gcaggatcag atttacagca aagatttacc gctgctgaaa aagatcattc ccgatcttaa
    12061 attcactgcc gctttcgggc gtgggcgcta cgtttgtccg cgtaatctga cggcgctcgc
    12121 cagcactgaa cccacgcaac aggatctgct ggcgtttctt gacgacgaac tgacgccaaa
    12181 taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc gatctcgaca cttataaatg
    12241 ggatggtctg cgcgatcata cggatatcgc tatagatgat gatctctggc gtcgtttgag
    12301 taccgacaaa gccagctgcc tcaaccgtaa ctgttactac tatcgtgaat gcccgttttt
    12361 tgttactcgt cgggagattc aggaagcgga agtggtggtg gcaaaccatg cgctggtgat
    12421 ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa aatctgctgc tggtgctgga
    12481 cgaaggtcat cacctgccag atgtggcccg ggatgcgctg gagatgagcg ctgaaatcac
    12541 cgcgccctgg tatcgactac agctggactt gttcacgaaa ctggtcgcta cctgcatgga
    12601 gcagtttcgc ccgaagacca tcccaccgct ggcgatccct gaacgtttga atgcgcattg
    12661 tgaagagttg tatgagctta tcgcctcgtt aaacaacatt ctcaatctct acatgcctgc
    12721 cgggcaggag gcagagcacc gttttgcgat gggcgaactg cctgatgaat tgctggagat
    12781 ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt ctggcggagt tatttcttaa
    12841 cgatttaagt gagaaaaccg gcagccacga tattgtgcgt ctgcatcggt tgattttaca
    12901 gatgaaccgc gcgctgggga tgtttgaggt gcaaagcaaa ctctggcggc tggcgtctct
    12961 ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg cgggaagagc gcgaagggca
    13021 gctacacctc tggtttcact gcgtgggaat acgtgtcagc gatcagctgg aaaggctgct
    13081 gtggcgcagt attccgcaca ttattgtcac ctccgcaacc ttgcgttcgc tgaatagttt
    13141 ttcgcgtttg caggagatga gtggtctgaa agagaaagcg ggcgaccgtt ttgtggcgct
    13201 ggattccccc tttaaccact gcgaacaggg caaaattgtt attccccgga tgcgctttga
    13261 gccttccatc gacaacgaag agcaacacat tgccgaaatg gcggcctttt tccgtgaaca
    13321 ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt gccagcggac gggcgatgca
    13381 gcgctttctc gactatgtga cggatttacg cctgatgttg ctggtgcagg gcgatcagcc
    13441 gcgttatcgc ttagttgaac tgcaccgcaa acgcgttgcc aacggtgagc gtagcgtgct
    13501 ggtgggctta cagtcatttg ccgaagggct tgatttgaaa ggtgatctgc tcagccaggt
    13561 gcatatccac aaaatcgctt ttccgcccat cgacagcccg gtggtgatca ccgaagggga
    13621 atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa agcctgccga gcgcctcgtt
    13681 taacctgatt cagcaggttg ggcgactgat tcgaagccac ggttgctggg gcgaagtggt
    13741 tatctacgac aaacgcttgc tgacaaaaaa ttatggcaag cgactactgg atgcattacc
    13801 ggtatttccg atagagcaac cggaagtccc tgaaggtata gttaaaaaga aagaaaaaac
    13861 gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca ggtaaggagt cgtaaatgga
    13921 ctatcgcaaa ataattaaag agatcgggcg cgggaaaaac cacgcgcgcg atttagaccg
    13981 ggatactgcg cgcggtctgt atgctcatat gctcaatggt gaagtccctg accttgagtt
    14041 gggcggcgta ttgattgcgc tgcgtatcaa aggggaaggg gaagcagaga tgctcggctt
    14101 ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg cctgcaggca agccgatgcc
    14161 gattgtcatc cccagttaca acggcgcgcg taaacaggct aacctgacgc cattattggc
    14221 gattctgctg cataaactcg gttttcctgt ggtggttcac ggagttagcg aagatccaac
    14281 tcgcgtgctg acagaaacca ttttcgaact gatgggtatt acaccaacgt tgcacggcgg
    14341 gcaggcgcag gcgaagctcg acgaacatca accggtgttt atgcctgtcg gcgcgttttg
    14401 cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg ggcgtgcgta acagtgcaca
    14461 taccctggcg aaactggcga cgccatttgc cgaaggtgag gcactgtgtc tttccagcgt
    14521 ttcgcatccg gaatacattg gacgcgtcgc gaagttcttt agcgatatcg gcgggtgggc
    14581 gttactaatg catggtactg aaggcgaagt gtatgctaat ccgcagcgct gcccgcagat
    14641 caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa aaacaggacc ctgctggtag
    14701 cgagttactg ccacaagcaa aagatccgga aaccacggcg cagtggactg agcgttgcct
    14761 ggctggcagc gaagcgattc ccgaatcgct gaaaatccag atggcttgct gcctggtggc
    14821 tacgggtgaa gtggcaacta tcagcgatgg cctggcgcgc gttaatcagg cattttaatt
    14881 tttatctcag ccggatgagc cacgtcatcc ggcttttacc ccaccgcaat atgaaattcc
    14941 tgcatcttta ttgaccttcc cgcgtccggc gtgcagcata aaaaaacaac aaacacataa
    15001 cataaacagg agttaaccat ggaaagtggt catcgctttg atgctcagac gctgcacagt
    15061 tttattcagg ctgtatttcg tcagatgggt agcgaggaac aagaagcgaa attagttgcc
    15121 gatcatttaa tcgcggcaaa cctggcaggg catgattctc atggtattgg catgatccca
    15181 agctatgtgc gttcctggag tcaggggcat ctgcaaatta accatcatgc caaaatcgtt
    15241 aaagaggcgg gggcggcagt cacgctcgat ggcgatcgcg cattcggtca ggtcgcggca
    15301 cacgaagcga tggcgctggg gattgagaaa gcgcatcagc acggtattgc tgccgtggcg
    15361 ctacataact cgcatcatat cggccgtatc ggttactggg cggagcagtg ttcagcggcg
    15421 gggtttgtct ctatccactt tgttagcgtg gtcggtattc caatggtcgc gccgttccac
    15481 ggtcgcgaca gccgctttgg caccaatccc ttctgtgtgg ttttccctcg taaagataat
    15541 ttcccgctgt tgcttgatta cgccaccagc gccattgcat ttggcaaaac ccgcgtcgcc
    15601 tggcataaag gcgtccccgt gccgccaggt tgcctgattg acgttaacgg cgtgccgacg
    15661 accaatccgg cggtaatgca ggagtcgccg ttggggtcgc tgttgacctt tgccgaacat
    15721 aaaggctacg cccttgcagc gatgtgtgaa attcttggcg gggcgctttc tggcggtaaa
    15781 acgacgcatc aggaaacgtt gcaaaccagc cccgatgcca ttcttaactg catgaccact
    15841 atcatcatca acccggaact gttcggcgcg ccggattgta acgcgcagac cgaagccttt
    15901 gccgagtggg tgaaagcctc gccgcatgat gacgataagc cgattttgct accgggcgag
    15961 tgggaagtga acacgcgtcg cgaacggcag gagcagggga ttccattgga tgcgggaagc
    16021 tggcaggcca tttgtgatgc ggcgcggcag attggtatgt cggaagagac gttgcaggct
    16081 ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag tggcggacgg gcaaacaagg
    16141 gtaacatagg atcaatgagg gttagagcat atgcgtctgt cggcaaacag acagggaaat
    16201 acttgtgctg gacgtagcgt aaacgcctga tccgtccaac ggttcggatt ttgtaggcct
    16261 gataagacgc ggtagcgtcg catcaggcat ctgttgccgg atgcggcata aacgccttat
    16321 ccgtcctacg aattacttat aaattaccgc agtaccgctt aatttattgt tgttggtggc
    16381 ggaagtgatg ctgtatccac tggcaccggc tgccgccgct ttctcagcca gtttcgcttc
    16441 cagggcatcg agggtggatg cgccatcggc agaaaccacg ccgattttat tcatgttctg
    16501 tgcctgggat gccgttaccg gttccgccgc gaacacgcca aatgacagag ttgaaagagc
    16561 catagcagca acaacagtat tgatagtttt cataattaat ctctcgcagg tgatttttgt
    16621 ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt tttttacgag agataaaatc
    16681 gaagagaatt gaccgccttg ttcaaataaa ttgattgata tctaacatac agataaacca
    16741 tttatttgtt gtacttatgg acttgtttta tgccgcgtgg cagtcacagc agcgttaaag
    16801 gcacggaatg ccacgcaaaa tacaaattat actcgctatg catctcgcca gttgtagtta
    16861 acgtaactgg ctgtctttcg aacctcagcg attatatcct gtatacgctg gtttttgtaa
    16921 atctttctcc tgctgacaat gaatacataa gcgcacgcca ggaatggctt cccgacgggc
    16981 ttgcgggatg ggggcaccgc actcttcaca ttcatacagg ctttcgccac gcggaatttc
    17041 accccgagcg cgggcaatcg catcttcaat tgtactgttg atctgttcgt tgacggcgtc
    17101 atcgttagcc caagcggatg ccatatcgtc ctccccatat caatgcttgt acagttaagt
    17161 gtagctaata tagggacgaa cctgggcagt tcaagcgtca gatctccgac cattcccgca
    17221 gcagattatg ataaagattg agcagcgaca ggatctcttc attttcacca tagcggcttt
    17281 tcagcgactg aatatttttg tccagttcaa acagcatggc gcgcttttta tcatcgcgga
    17341 tcatcgactg gatccacata aatgatgcca ctcgtacgcc gcgggttacg ggtgtcacgc
    17401 aatgcaggct gctggaggga tacaacacca gatcacctgc cgggagtttt acccgatgtt
    17461 gtccaaaggt gtcattaacg accagttcgc cgccgtcgta gctttgtgga tcgcttaaaa
    17521 acagcgtggc agaaaggtca gtgcgcatcc agccgttttg tggatggctg cgtactgcgc
    17581 catccacatg gaaaccatag gtttcattgt tctgatagcg attaaacagc ggcgtggaaa
    17641 gggtacgcgg caaggcagcg gcaaagaaca atgcatgttg gttaaccgcg ttcagcacct
    17701 cattttgcag tgcggcgtat aacgcgctgc gggtgtctac ttgctgattg ttcttaactt
    17761 gtgcaccttg tgctccggtg gtgacgcgtc catccaccca ttcggcttgt tccagttgtt
    17821 cgcgaaaatg ggcgacgtcc tgtggcgata acacgccggg aatgtggtac atcatacaag
    17881 ttctccaaaa agtggggcct gcgccccaca tctgaatcag aaatgcatat tggctgtgag
    17941 caagaaggtt cttggctcgc ccgggtgata acggtagccg ctcttgttga ttgaggcgac
    18001 gtaatcggta tcaaacaggt tgtaaacgtt tagctggaag tcgagattgc gattaacccg
    18061 ataccccagt ttggcatctg cgacccagta accttctgta aatgctggcg ttcccaccgc
    18121 gccgtctgaa cctttatgta tactgccgat atagcgtgcg cccgcgccaa cagagatatc
    18181 atcggttgcc tgatattggc tccataaggt gaaggcgtgc tccggggtat acggcagcga
    18241 tgaggaacca tcctgggcaa catctttgcc gtttttgatg gttgcttttt gctgggtata
    18301 gccgccaatc acctgccacg cgggagtgat attccctgcc acggatatct catagccttc
    18361 gacgcgtttc ttaccgtatt gcgagtaagt cccgtcatca ttttgctcaa cttcattttc
    18421 gatatcagta cggaacagcg cggcggtgag caacaggcgt ttatccagaa cctgccattt
    18481 ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa tcggtgcggt tggcactgtt
    18541 accactgcca gactgcgcaa gggcgaagtt gttgccgccc ggaggctgct gggaaacggc
    18601 atagttaata tagacattgc cgttttccgt caggtgatac agcgccccgg ctttccagtt
    18661 caccagattg cccgacttgg cggtgtcgac ggtggtgact ggagaacctt ttgccacacc
    18721 agctgggcag gtgatggcac cgcgtccgct gccgccgcag gtggtggcac tgtcatattc
    18781 agtatgataa ttatccagac ggatcccgcc gttcagctca aaatcacggg tgatttgcag
    18841 cgtatcgaag gcgtaaattg cgaaggtatc cgtctgacca ttggcgtttg cgccgttgcg
    18901 cgtcaggccg ccgggatgaa ttctgctgtc aggatgataa atatttaccg ccggtaacgt
    18961 caccggatta acgccatagt tagtctgcgt ttcacgggta aattccacgc cggtactgac
    19021 atcatgaccg atagaaccgg tatagaacgt cgaggtcagg ttggtctggt tggtgagaat
    19081 tttattactc acatctttga tattcgccgt gcgtgactag gtccagctat tcacatcgct
    19141 ggtgggctga gtaatattcg acgccccgcc cataatcgcc gtcatcaggt aatcctgctt
    19201 tacgcgcgac caacgggtag tattgcgaat ggtggtgtta tcgttgatgt cgtgctcaaa
    19261 acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg gaatccgtgc cgtaaaagtt
    19321 atgagtatca acttttccgg aatgattcag ggccgccgct cccgcagatg gggcagaata
    19381 gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg ttgtgctgag tgacatgcag
    19441 ataattaaga tacaaacgat tcgctgtacc aaggccaaaa gcgacagaag gggcgacgcc
    19501 gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgg gttttttcgc ccatcacatt
    19561 caggcgcacc gcggtagtat caccaatgac ctgattgacg tccagcgtgc cgcggcggaa
    19621 ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa tcattgcgcg gctgcttgct
    19681 gatcatattg atcgagcctg tcggtgcgct gcgcccgtag tcggtgccgg acggcccttt
    19741 aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag acgctgccga tatcgcgaat
    19801 accatcaata taaatactgt tagaggtatc ggcaccacgc atataaatgg cgtcgccagt
    19861 ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg c
//
LOCUS       DAJTYG010000071        19778 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTYG010000071 DAJTYG010000000
VERSION     DAJTYG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.071"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..441
                     /gene="feaR"
                     /locus_tag="OO568_002710"
     CDS             <1..441
                     /gene="feaR"
                     /locus_tag="OO568_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HCR6642008.1"
                     /translation="QESMNNPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQ
                     KVVTLIDDNIREEILRPEWIAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAI
                     RHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(613..942)
                     /locus_tag="OO568_002711"
     CDS             complement(613..942)
                     /locus_tag="OO568_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6642009.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(947..1132)
                     /locus_tag="OO568_002712"
     CDS             complement(947..1132)
                     /locus_tag="OO568_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6642010.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(1129..3768)
                     /locus_tag="OO568_002713"
     CDS             complement(1129..3768)
                     /locus_tag="OO568_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6642011.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLDVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHVGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3976..4965
                     /gene="ldhA"
                     /locus_tag="OO568_002714"
     CDS             3976..4965
                     /gene="ldhA"
                     /locus_tag="OO568_002714"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6642012.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAVLELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5076..5412
                     /locus_tag="OO568_002715"
                     /pseudo
     CDS             5076..5412
                     /locus_tag="OO568_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
     gene            complement(5409..5675)
                     /locus_tag="OO568_002716"
     CDS             complement(5409..5675)
                     /locus_tag="OO568_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6642013.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5949..9473
                     /gene="nifJ"
                     /locus_tag="OO568_002717"
     CDS             5949..9473
                     /gene="nifJ"
                     /locus_tag="OO568_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6642014.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAGAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9840..10969
                     /gene="ompN"
                     /locus_tag="OO568_002718"
                     /pseudo
     CDS             9840..10969
                     /gene="ompN"
                     /locus_tag="OO568_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            11110..11544
                     /gene="uspF"
                     /locus_tag="OO568_002719"
     CDS             11110..11544
                     /gene="uspF"
                     /locus_tag="OO568_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6642015.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11720..12655
                     /gene="ttcA"
                     /locus_tag="OO568_002720"
     CDS             11720..12655
                     /gene="ttcA"
                     /locus_tag="OO568_002720"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6642016.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12787..14154)
                     /gene="dbpA"
                     /locus_tag="OO568_002721"
     CDS             complement(12787..14154)
                     /gene="dbpA"
                     /locus_tag="OO568_002721"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6642017.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(14632..15615)
                     /gene="zntB"
                     /locus_tag="OO568_002722"
     CDS             complement(14632..15615)
                     /gene="zntB"
                     /locus_tag="OO568_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6642018.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIISTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            15870..17102
                     /gene="dgcM"
                     /locus_tag="OO568_002723"
                     /pseudo
     CDS             15870..17102
                     /gene="dgcM"
                     /locus_tag="OO568_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(17123..17686)
                     /gene="smrA"
                     /locus_tag="OO568_002724"
     CDS             complement(17123..17686)
                     /gene="smrA"
                     /locus_tag="OO568_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR6642019.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17940..19394)
                     /locus_tag="OO568_002725"
     CDS             complement(17940..19394)
                     /locus_tag="OO568_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR6642020.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGILQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(19391..>19778)
                     /locus_tag="OO568_002726"
     CDS             complement(19391..>19778)
                     /locus_tag="OO568_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6642021.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         5198 a         5007 c         4667 g         4906 t
ORIGIN      
        1 caggagagca tgaataatcc ggcactttct gaaacagaaa gtgaagctgc gctacaggcg
       61 atggtgtgtc tgcttcgccc agtacttcat cagcgggaat ctgtccaacc tcgccgtgaa
      121 cgtcagtttc aaaaagtggt tacgttgata gacgataata ttcgcgaaga gatattacgc
      181 ccggagtgga tagccggaga gacaggtatg tcagtacgta gtttgtaccg aatgtttgcc
      241 gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc gtctcgattt ttgtgcagat
      301 gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca tcggctttca ttggggattt
      361 tctgaccaga gtcatttctc aacggtattt aagcaacgct ttgggatgac gccaggcgag
      421 tatcgccgta aattccgcta actaattggc gttgcatgta ctgcaacgcc aattagttaa
      481 aacagatatt tgactatgca tttttcaggg cgaaaggcaa tttgacagga gtttttgaat
      541 attcaccttg tttcaggaaa atagatcaat cgctttctcc tgtagcgtta tcccgatatt
      601 gcggttttgc tattaaaact ttcgcaccca tttaccgttt atcccttgta catattcccc
      661 cggtttggcc cgagctacga gcttttgccc ggcgagtttc gcgatatcat ctacagacac
      721 attattttgc tttgcccgtt gttggtaact cgctttacgt tcggcattaa tttcggttac
      781 taatttctct gtttcagcat ccgttttcag cgcaaccaga taaccataaa atgtttcacc
      841 tacccgcccc tgagttctgg cttcatccag agtcaatgcc attacattgc tgcttaccag
      901 cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc atcgcctcag aaaagatcgc
      961 tacgagtttc aagcagctct tcgacatctt tgtctgcctt gatgatgatc tcatgctcaa
     1021 ttttaacgtt catattgata gtgatcggtt ccttaggtgc agcgacttca atgcgaggcg
     1081 tacagccaac cagcataaaa gatgacgtca acgcagccag taaaattttc attgtttttc
     1141 ctcacactct tttccttgcg gacagtcatt tcccggcaga cgtgcgttct gctccaacca
     1201 tgcctggaga ttatcgccaa agcgtaaact gcgccacagc gtaaaaatat tctcttcatg
     1261 atggtaatta aggtttaccg taccgctttt accatcaacg cgactggtac ctgtaatgtt
     1321 ggcctgcatg gttaataaac cgagattatc taagttaatt tttgtcgatg accggctaat
     1381 ttccatatag cgcaaccagt taattgctga acccgcagtc atattgtctt tcaccaccgc
     1441 atctgcggta tctttatcca gccgcaaggt cattggcccg ctatttgcca gccaaccatc
     1501 tttcactatc catttttcgt tgtttagcca taacggcaat gcaccactaa atgccccgga
     1561 catggcgaat tgtttcggat tgacggcgct aaccagttcg ctggatgaga gattattcag
     1621 ccgcaacagc gccgggtcat gttgcggcat acgtaattgt ttcatcagca cgttaccgcc
     1681 taacacatcg acgctaacat cagtcaacag caagggttct tcttcagtcc acggataacg
     1741 cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga ttaatcactt cggcaattcg
     1801 caacgtaacg gggccgcgag tccccagatg ccaggctcca tcggcaaaac ggaaaggcag
     1861 gacaaaatcg acaccgttaa cctgattatc tggcatccag gcactaccgc ctttcaacac
     1921 gccgtgccct cccgcgcgga atccttgttc aggcgcagca gaaaatgcaa cctgagcata
     1981 cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc accagcggct ggaatacagt
     2041 cagtgattgt tttggccacc aggcgttacc gcgcagacga ataccgtccc agcgaccatt
     2101 tacccgaacc gggccaatct caccaacatg taaatcgcct ttaaagagga aataggtcgg
     2161 atcgcgccca tcgacgctaa attttaaggt tgatggcggt aacacactgc cgccagtgaa
     2221 cagcgtttgc cctgcgtcca gtgacagcgc gccgctgaag gagggatgct gtgcgtcacg
     2281 tacccagacg acgggcttgt cgagaattaa tcgcggcttt tctaccgtca tcgtaccgta
     2341 ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta atcgtgctgt catgccactc
     2401 accttttcct gcgacatccc aggtggcatt catcggtgta aaactccctt ttccccagta
     2461 gcgccactgc cagcggccag cgtcagggag aaaatcattc gccagcccat ccatatgcag
     2521 cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg gcctgcaaac gtccgtcaac
     2581 accacgttgg gtgactttta cacccgctaa aggccagcgg atttcatcga tgtccagcga
     2641 atcgatgact cttccctttg aacgaagtaa cgcgcctggc tcaaaggtca gcgttgggtc
     2701 agacagactt ccacttagct gcgcaggtaa acgggcatat aaaatgaggt ccgcctgttt
     2761 cgcttcaccg gtcagctgca gaggcagttg actgttatcc atgcttaatt ttcctgggcc
     2821 aaaattaagc accgcgttgc ccttacccgc ttgcccctgg gtcagcacac tcagtcgtcc
     2881 gctgaccaga gcgttctcaa gccctgcctg ccagttgtca actttgacat ccagtcggcc
     2941 actcaaagga aaacctgcat acagccagct ccagcgacca tcgcttacgg tcaattgttg
     3001 acgagtaatt tgccacggca aatcgagcaa cggatcgccg ttatcccgtg ccagcacgat
     3061 caattgcccg ctattttcct gccagtccag ctcggcatcc accagtgacg gttcctgcgg
     3121 caagtttaac gtcgcagtag catgcccact tacaggaagt ccatccggca cgagcggcat
     3181 agtaaattcc cccaccagtt taaccggcgg ctgattttca aacgcgacga catccagttc
     3241 gctgactgta agttgttgcc ctttcagctg gccttgaaat ttaacttttt cgccctgata
     3301 acgcagttgc tggatatcag aggttaatgc gagagagagt tttccctgcc attcctgcca
     3361 gggagaaaaa atcagtttat cgatattgat ccaggtgtta ggcagcatgg actgccactg
     3421 cgcgagggtt tttggagcag ctggcgattg ctccgtctgc ggcaatttcg ccagacaagc
     3481 agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg ctgggatgtg aaaggctggc
     3541 gttggtgata tgcgcaagct gacaatctcc caccagataa cggagatcgg gaatgattaa
     3601 acctttacgc gtaatgcgtg ggctttcatc taatgcaata cgtgtcccga gcggtagcca
     3661 gatgtccgcc agcgtgggaa cccacagccc gagcgtcatc agcagcgtca acggcacaag
     3721 aataatcagt aataacagcg cgagaacggc tttatattta cccagcatgg gtagttaata
     3781 tcctgatttc gcgaaaaatt aagcattcaa tacgggtatt gtggcatgtt taaccgttca
     3841 gttgaaggtt gcgcctacac taagcatagt tgttgatgaa tttttcaata tcgccatggc
     3901 tttcaattat atttgaaatt ttgtaaaata tttttagcag cttaaatgtg attcaacatc
     3961 actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga caagaagtac
     4021 ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt tctgctgacg
     4081 gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt aaacgatgac
     4141 ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat cgccctgcgc
     4201 tgtgccggtt tcaataacgt cgaccttgat gcggcaaaag aactggggct gaaagtagtc
     4261 cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat gatgatgacg
     4321 ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt ctctctggaa
     4381 ggtctgaccg gctttactat gtatggcaaa acggcaggcg ttatcggtac cggtaaaatc
     4441 ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc gttcgatccg
     4501 tatccaagtg cagcggtgct ggaactcggt gtggagtatg tcgatctgcc aaccctgttc
     4561 tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta ccatctgttg
     4621 aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac cagtcgcggt
     4681 gcattgattg attctcaggc ggcaattgaa gcgctgaaaa atcagaaaat tggttcgttg
     4741 ggtatggacg tctatgagaa cgaacgcgat ctgttctttg aagataaatc caacgacgtg
     4801 atccaggatg acgtattccg tcgcctgtct gcctgccaca acgtgctgtt taccgggcac
     4861 caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct gcaaaactta
     4921 agcaatctgg aaaaaggtga aacctgcccg aacgaactgg tttaatcttg ccgctcccct
     4981 gcaatccagg ggagctgatt cagataatcc ccaatgacct ttcattctct attcttaaaa
     5041 tagtcctgag tcagaaactg taattgagaa ccacaatgaa gaaagtagcc gcgtttgttg
     5101 cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa aattgctgtt acgccagaaa
     5161 tcagctttgg tgaaaaaatg atgatttccg gcagcatgtg taaccgcttt agcggtgaag
     5221 gcaggtgaac tgacagccaa agggctggca atgacccgta tgatgtgcgc taacccgcag
     5281 cttaatgaac tcgataacac cattagcgaa atgctgaaag aaggtgcaca agtggatctg
     5341 accgcgaacc agttaacgct ggcgaccgcg aaacagacat taacttataa gctggcggat
     5401 ttaatgaatt aatagctgcc acagctcccg gcggcaagtg actgttcgct acagcgtttg
     5461 ccgttgggta atgcacacat cccaatcgcc gtaccatcca gttgacgggc aacagaaagc
     5521 gaaccgccga tcattgcaca atttgcttct ccactactgg acatcgacgc ttttaaactt
     5581 ggcgctacgt gcgccgcagt cgcctgctga acaggctcac tactacacgc cgacaacaat
     5641 aaagcggcac accctaccca aaacgctgct cgcatctctt cttcctctga tcttcaagcc
     5701 aaacgacacc gccataaata ataggcagca cagagggcgg cgtcgagagc tgtcctgcgc
     5761 gttgccccgc atttttactt ttttatggct atttttttgc cctctgtttg atcaaaacat
     5821 tcattactct gatgtggggg acacaaaagc gaaaatgcag aagaaagcca tttgctaaaa
     5881 ttgaaggatt actactgggc gcgcagcaat ttcgtgcgcc cctcatttgc gcaatgtaag
     5941 ggtgtcatat gattactatt gacggtaatg gcgcggttgc ttcggtggcg tttcgcacca
     6001 gtgaagttat cgccatctac cctattaccc ccagttccac gatggcagaa caggctgatg
     6061 cctgggccgg aaacggcttg aagaacgttt ggggagacac accacgcgtg gttgaaatgc
     6121 agtcggaagc gggtgctatc gctaccgtgc atggcgcttt gcagacgggt gccctttcaa
     6181 catcgtttac gtcatcgcag ggtttgctgc tgatgatccc gacgctgtac aaactggcag
     6241 gcgaactgac gccatttgtc ctgcatgtag cggcgcgtac cgttgccaca catgcacttt
     6301 ctatttttgg cgatcattcc gacgttatgg cggtgcgcca gacgggttgc gcgatgttgt
     6361 gtgcggctaa cgtccaggaa gcgcaagact ttgctctcat ttcgcacatc gcaacgctga
     6421 agagccgcgt gccatttatt catttctttg atggttttcg cacgtcccac gaaatcaata
     6481 aaattgtccc gctggccgat gacactattc ttgatctcat gccgcaggcc gaaattgatg
     6541 ctcatcgcgc ccgggcgctc aacccggaac atccggtgat ccgcggtacg tccgccaatc
     6601 ctgacactta tttccagtct cgcgaagcca ccaacccctg gtacaacgcg gtctatgacc
     6661 atgttgaaca ggcgatgaat gatttcgctg ccgcgacagg tcgtcagtat cagccgtttg
     6721 agtattacgg gcatccgcaa gcggaacggg tgattatcct gatgggctct gccattggca
     6781 cctgtgaaga agtggttgat gaattgctaa cccgtggcga aaaagttggc gtgctgaaag
     6841 ttcgtctgta ccgtcccttc tccgccaaac acttgctgca agctctgccg ggatccgtac
     6901 gcagcgtggc ggtactggac agaaccaaag aacccggtgc ccaggcagaa ccgctctatc
     6961 tggatgtgat gaccgcactg gcaggagcct ttaataatgg tgagcgcgaa accctgcccc
     7021 gtgtcattgg tgggcgctat ggtctttcat ccaaagaatt tggcccggac tgtgtactgg
     7081 cggtatttgc cgagctcaac gcggctaaac ctaaagcacg ctttacggtt ggtatttacg
     7141 atgatgtgac caatctgtca ctgccgttgc cggaaaacac tctgccaaac tcggcgaaac
     7201 ttgaagcctt gttttatggc cttggtagtg atggcagcgt ttccgcaacc aaaaacaata
     7261 tcaagattat cggtaattcc acgccgtggt acgcacaggg ctattttgtt tacgactcca
     7321 aaaaagcggg cggcctgacg gtttctcacc ttcgcgtgag cgaacagccg attcgttccg
     7381 cttatctcat ttcccaggct gattttgttg gctgccacca gttgcagttt atcgataaat
     7441 atcagatggc tgagcgttta aaacctagcg gcattttcct gcttaatacg ccgtacagcg
     7501 cagatgaagt gtggtcgcgc ttgccgcagg aagttcaggc cgtgttaaac cagaaaaaag
     7561 cgcgcttcta tgtgattaac gcggcgaaaa tcgcccgcga atgtggcctg gcggcccgta
     7621 ttaataccgt catgcagatg gcttttttcc atctgacgca aattctgcct ggcgatagcg
     7681 ccctcgcaga attgcagggt gcgattgcca aaagttacag tagcaaaggt caggatctgg
     7741 tggagcgcaa ctggcaggct ctggcgctgg cgcgtgaatc cgtagaagaa gttccgttgc
     7801 aaccggtaaa tccgcacagc gccaatcgac cgccagtggt ttccgatgcc gcccctgatt
     7861 tcgtgaaaac cgtaaccgcg gcgatgctcg ccgggcttgg tgatgccctc cccgtttcgg
     7921 cgctgccgcc agacggcacc tggccgatgg gcaccactcg ctgggaaaaa cgcaatatcg
     7981 ccgaagagat ccccatctgg aaagaggaac tctgtaccca atgtaaccac tgcgtcgccg
     8041 cttgcccaca ctcagctatt cgcgcaaaag tggtaccgcc tgaagcgatg gaaaacgccc
     8101 ctgccagcct gcattcgcta gatgtgaaat cgcgtgatat gcgcgggcag aaatatgtct
     8161 tgcaggtggc accggaagat tgcaccggtt gtaacctgtg cgtcgaagtt tgcccggcga
     8221 aagaccgtca gaatccagag attaaagcca tcaatatgat gtctcgccag gaacatgtcg
     8281 aagaagagaa aatcaattac gatttcttcc tcaacctgcc agaaatcgac cgtagcaaac
     8341 tggaacgtat tgatattcgt acatcgcagc tgattacacc gctgtttgaa tattcaggtg
     8401 cttgccccgg ttgtggcgag acgccgtata ttaaattgct gactcagctc tatggtgacc
     8461 ggatgttgat cgctaacgcc accggctgtt cttcaattta tggcggtaac ctgccctcta
     8521 caccgtatac caccgatgcc aacggtcgtg ggccggcatg ggcgaactct ctatttgaag
     8581 ataatgccga atttggcctt ggtttccgcc tgacggtcga tcaacaccgt gtccgcgtgc
     8641 tgcgtctgct ggatcaattt gccgataaaa tcccggcgga attactgacg gcgttgaaat
     8701 cagacgctac gccagaggtt cgtcgagaac aggtcgcagc tttacgccag caactcaacg
     8761 atgttgccga agcacatgaa ctgctgcgtg acgcagatgc actggtggaa aaatcaatct
     8821 ggctgattgg tggcgacggc tgggcttacg atatcggctt tggcggtctg gatcatgtat
     8881 tgagtttgac ggaaaacgtc aacattctgg tgctggatac gcaatgctat tccaacaccg
     8941 gtggtcaggc gtcgaaagcg acaccgctgg gtgcagtaac taagttcggc gagcacggaa
     9001 aacgtaaagc gcgtaaagat cttggcgtca gtatgatgat gtacggtcat gtttatgtgg
     9061 cgcagatttc tctcggcgcg cagctgaacc agacggtgaa agcgattcag gaagcggaag
     9121 cgtatccggg accatcgctg atcattgctt atagcccgtg tgaagagcat ggttacgatc
     9181 tggctctcag ccacgaccag atgcgccaac tcactgctac cggcttctgg ccgctatatc
     9241 gctttgatcc gcgtcgtgcc gatgaaggca aactgccgct ggcgctggat tcacgcccgc
     9301 cgtcagaagc actggaagaa acgttacttc acgagcagcg tttccgtcgt ctgaattcgc
     9361 agcagccaga agtggcagaa cagttatgga aagatgctgc agctgatttg caaaaacgct
     9421 atgacttcct agcacaaatg gccggaaaag cggaaaaaag caacaccgat taaatgctct
     9481 ggataaggat tatccaattc taaaaaaaaa gcccggacga ctgttcgggc ttgtcttttt
     9541 atatgttgga aaatcagtgg caatgcaatg gcccaacaga aaataaaatc tgtgacaata
     9601 aaggcatata acccgcgcag aataacgtat agaaaattaa aagtataatt tttattttgt
     9661 atgtattatc ttcgatacat tcaccagact tattatcatt tcagaggaat tatttgatta
     9721 aggttttact taaggcgtaa caaatgatat ttatcggcaa actgaacttc tcctttagga
     9781 tgttttcact ccccgaaatg gggataaaag gcaaataaaa taactaaagg atttattcaa
     9841 tgaaaagaaa agcactggca cttttaatcc ctgccctgct cgccgcaggt gctgcacatg
     9901 cagccgaagt ttataataaa gacggcaaca aattagatct gtatggcaaa gttgatggcc
     9961 tgcattattt ttctgataat tcagcgaaag atggcgacca gagctatgcg cgtctgggtt
    10021 ttaaaggcga aacccaaatt aacgatcaac ttactggcta cggtcaatgg gaatacaata
    10081 ttcaggcaaa caacactgaa tcttcaaaaa accagtcatg gacccgtctg gcatttgccg
    10141 gactgaaatt tgcagattac ggttctttcg attacggacg taattatggc gtaatgtacg
    10201 acatcgaagg ctggaccgat atgctgccta aatttggcgg tgactcttat accaatgcag
    10261 acaactttat gactggtcga gccaatggcg tcgcgactta tcgtaatact gatttcttcg
    10321 gtctggtaaa tggtctgaac ttcgcggtgc agtatcaagg taacaacgaa ggagccagta
    10381 atggtcagga aggcaccaat aacgaacgcg atgttcgtca tgaaaacggt gacggctggg
    10441 gtctttccac aacatatgat ttaggcatgg gctttagcgc tggtgcggca tacacctctt
    10501 ctgaccgcac caatgaccag gttaaccata ctgcggcggg tggtgataaa gcagacgcgt
    10561 ggactgctgg gctaaaatac gatgctaaca atatttacct agcaaccatg tattcagaaa
    10621 cgcgtaatat gaccccgttt ggcgacagcg attatgcagt tgcgaacaaa acccagaact
    10681 ttgaagttac tagtaccagt ttgattttgg tctgcgtccg gcagtctctt tcctgatgtc
    10741 taaaggccgt gaccttcacg ctgctggtgg tgcagacaac ccggcaggtg ttgatgataa
    10801 agatctggtt aaatacgccg atgttggcgc gacttactat ttcaataaaa acatgtccac
    10861 ctatgttgac tataaaatca acctgttgga tgaagatgac agtttctacg ctgccaacgg
    10921 catctctacc gatgatattg tcgctttagg tctggtttat cagttctaaa tcctcctgcc
    10981 cgctgttatg gcgggctttt tctgcttatt cttcctcctt tgatctaaat taaaaatgtg
    11041 aactccgtca ttacacaaaa agtgtcatct ggcgttacac tttatgcgga tactaaaaca
    11101 ggaggtttta tgaacagaac gattcttgtc cctatcgata tttcagattc agaattaact
    11161 caacgcgtga ttagccacgt tgaggcagag gcaaagattg atgatgcaga ggttcatttc
    11221 ctgacggtaa taccttcact gccctactat gcctctctgg gtttagcgta ttccgcagaa
    11281 ttaccggcaa tggatgacct gaaagcggaa gccaaatcgc aactggaaga gatcattaaa
    11341 aaatttaaac tgccaaccga cagagtgcat gtccatgttg aggaaggctc gcccaaagac
    11401 cgcattctgg aattggcgaa gaagatcccc gctcatatga tcatcattgc ttcccatcga
    11461 ccggatatca ccacttatct gctcggttcc aacgccgcag ctgtagtgcg tcacgcagag
    11521 tgctccgtgc tggttgtgcg ctgacactaa cgcccgcaca ttgctgcggg ctttttgatt
    11581 catttcgcaa atgtgctgac attttcccct ctaatccgta ccatacacgc cacagttttt
    11641 atatcagatt tcttatgctg agcgttccgg catgacgcat actcttctga tgccatataa
    11701 cgaattgagt cgcttttaaa tgtcgcaaaa tcaagaaatt agtaagaaag aacaatacaa
    11761 cctgaacaaa ttacaaaaac gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa
    11821 tatgattgaa gaaggcgatc gcatcatggt ttgcctctcc gggggtaaag acagctatac
    11881 catgctggag attctgcgca atttgcagca aagcgcgcca atcaattttt cgctggtggc
    11941 tgttaacctc gatcaaaagc aaccgggctt cccggaacac gttctgcccg agtatcttga
    12001 aaagctgggc gttgagtaca agattgttga agagaacact tacggtatcg tgaaagagaa
    12061 gattccagag ggcaaaacca cttgctcact gtgttctcgg ctacgtcgcg gtatccttta
    12121 tcgcaccgca acggaactgg ggacgacgaa gatcgcgttg ggtcaccatc gtgacgatat
    12181 cctgcaaacg ttgttcttaa atatgttcta cggcggcaag atgaaaggta tgcctccgaa
    12241 actgatgagc gatgatggca aacatatcgt tattcgtcca ctggcctact gccgcgagaa
    12301 agatattcag cgatttgccg atgcaaaagc gttcccgatt attccgtgca acctgtgcgg
    12361 ttcacagcct aacctgcaac gtcaggtgat tgctgacatg ttgcgtgact gggataaacg
    12421 ttatccaggg cgtatcgaga cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct
    12481 gtgcgatacc aacctgttcg atttcaaagg cattacccac ggttctgaag tggttaacgg
    12541 gggtgatctg gcgtttgatc gcgaagagat cccactacaa ccggcgggct ggcagccaga
    12601 agaagatgaa aatcagttgg atgagttacg gctgaatgtg gttgaagtga aataaccagg
    12661 atagcgcccg atgcgcaagc gtatcgggct actcttatgg aggccggata agacgcggcc
    12721 agcagcgtcg catccggcaa tcccgaataa gatgtttact cttgcacccg gcaattcaac
    12781 atttcattat tttaataacc gcacccggca cgtttttcct ttaatcttcc cgccctgtaa
    12841 ctgtttccat gctttatgag caacagcctg acggaccgcg acatagacat gcgccggatg
    12901 cacggcgatt ttgccaatat ctgcgccatc aagcccgata tctcctgtca gtgcacctaa
    12961 tacatcaccc gggcgcattt tggctttttt cccgccatcg atacacaacg ttgccatttc
    13021 tgcttccagc gtcacaatgg aactattagc tggcggcgtt tgccagttaa gttttatctg
    13081 caacatgtca gaaatgatat tggcccgctg tgcttcttcc ggagcacaga aactgatcgc
    13141 cagaccgcta tttcctgcac gagctgtacg accgatgcga tgtacatgaa cttcagggtc
    13201 ccacgccagc tcaaagttca ccaccagctc aagcgattta atatccagac cacgcgcagc
    13261 aacatcagtc gcaaccagta cacgggcgct accgttagca aaacgtacca gggtctgatc
    13321 gcgttgctcc agatcgccgt gtaacgacaa tgcactttgc tctacttcat tcagcgcgtc
    13381 gcagacagcc tggcaatctt ttttggtatt gcaaaacacc acacaagagg atggctgatg
    13441 caagcttaat aaccgttgca acagaggaat tttgccttta ctggatgtct cataaaattg
    13501 ttgttcaatg ggtggcaaag catctgttga gtcaatttca atcgccaaag gatcgcgttg
    13561 cactcgtccg ctgattgcgg cgatggcttc cggccaggtt gccgaaaaca gaagcgtctg
    13621 tcgagatgca ggcgcaaaac ggatgacatc atcaatggca tcgctaaatc ccatatccag
    13681 catgcggtcg gcctcatcca tcaccaaagt attcagcgca tccagtgata ccgtgccttt
    13741 ttgcaagtga tccagcaaac gacccggcgt tgccacgata atatgcggcg catgttgcaa
    13801 cgaatcacgc tgcataccga acggttgacc accgcacaac gtcaaaattt tggtatttgg
    13861 cagaaaacgc gccagccgac gcaattcacc tgccacctga tccgccagtt cacgcgtagg
    13921 acacagcact aaagcctggg tttgaaatag cgacgcatca atttgctgta acaagccgag
    13981 gccaaaagcc gccgttttgc cgctgccggt tttcgcctgc acgcgaacat cttttccggc
    14041 aaggatcgcc ggaagcgcgg cggcctgcac cggcgtcatg gttaaataac ccaactcatt
    14101 aaggttcgtg agttgggcgg gaggcaaaac attcagggta gaaaaagcgg tcacaatcta
    14161 ttctcgtggt catcgacgca aagttagcag gcgcgtatcc tcgcagatct acgctcacga
    14221 tgcgacaatt taatcggttc ttcatcgggt ggtgggtcag gcatgggttg cgggcgaggg
    14281 atcggatcgg gcactggaac aggatcgcca ggaatcggtt cagggacagg aatttgcaaa
    14341 taaataagtg tcgtcatatt tccctccggt cattgggtgg actcttaaag ggtagacgct
    14401 gataaataac aggcaaaaaa aagccgactc atcaaagtcg gcgtcgtacg aatcaattgt
    14461 gctatgcagt aattcaaaaa aggaagtaag acaatatgga gcgcaacgcc catcgcttga
    14521 cgttgcattc acctgcaagg gagatattgc cctgaatggg tagagagttt attgacttcg
    14581 ctcaaacttt gcggcgtttt tgtatacaga cagccggaaa aattgctttt gttacaacca
    14641 tttactacgg tgcaaccata aagcaacacc accaataaga acaactaaca gaatacaaaa
    14701 aattgaaaat ccgaattgcc acccgccgcc agggatccca ccaaggttga cgccaaataa
    14761 cccggtcaga aaggtactgg gtaaaaagac cattgccatc aacgacattg tataggtacg
    14821 acgagctaaa ttttcctgca tcacctgagc gatttcatcc gccatcacgc cagtccgtgc
    14881 tatacaggcg tcgatttcgt caaggccgcg cccaaggcga tcggcaatat cctgcatccg
    14941 acggcgttgg tcatcgctca tccacggcaa acgttcactg gcaagacgag cataaacatc
    15001 acgttgcggt gccatatagc gacgcatcac aattaattgt ttgcgcagca gagccaggaa
    15061 tccacgcggt ggaatttgct gatcaaggag attatcttca aggtcgataa ttttatcgtg
    15121 cagctgctcg ataaattcac tggaatgatc ggtcaacgca tcgcacacat ccaccagcca
    15181 tcccccgcaa tcggtcggac ccgtgccctc ttccagatcg ctcaccacat cgtccagcgc
    15241 cagcactttg cgttgtcggg tcgaaataat taaccgcccg tccatatata cacgcatggc
    15301 gaccagttga tcggggcgtt catcggtgct gccgtttata cagcgcaatg taatcagcgt
    15361 gccttcaccg aaacggctga ctcggggccg tgtgctctcg cccgccagcg catcacgtac
    15421 gttattggga agcagcggtg ttgtcgccag ccattgggcg ctatcatggt gtacataatt
    15481 aaggtggagc cagcagggat gcgcttcatc aatcacatct gtattttcca gcggtttaac
    15541 gccgcctcta ccatccagca tccaggcaaa tactgcatcc gggacattaa cgtccgatcc
    15601 cttaatcgct tccacagtgc ctccatcatc aacgcattat tttgtagtct agccttctgg
    15661 ccctgttacg caacatctca tcaccccatt accctgaaat gattaataaa attctgtcta
    15721 aattgaatac aaaaagcaaa atgcttttcc gtatacaaac cgtgtgaagt gttaaatagc
    15781 gtctatcatt atcagaatta tctgatcata tgacgtggct tttttgcgat cggatagcaa
    15841 caaaaattga taaaaataac gggatctcaa tgattacgca caacttcaat accctggact
    15901 tactcaccag tcctgtctgg attgtttcgc cctttgagga acagttaatt tatgccaata
    15961 gcgcggcgcg actgttgatg caagacctca cgtttagtca gctacgaacc ggaccctatt
    16021 ccgtctcctc acaaaaagaa ctgccgaaat acctctccga tctgcaaaac caacacgata
    16081 ttatcgaaat cctcactgtt cagcgtaaag aagaggaaac agcattaagc tgtcggcttg
    16141 ttttgcgaga gctgacagaa acagaaccgg tgattatttt cgaaggtatc gaagcgccgg
    16201 caacgctggg tttaaaagcc agtcgctcgg caaattatca gcgcaaaaaa caaggttttt
    16261 atgcgcgctt ttttctgact aactctgcac caatgttgtt gattgacccg tcacgagatg
    16321 gacaaatcgt cgatgctaac ctcgccgcgc tcaatttcta tggttataac catgaaacga
    16381 tgtgccagaa acatacctag gaaataaata tgcttgggcg tcgcgtcatg cctatcatgc
    16441 atgaaatttc gcatttaccc ggcggtcata accctttgaa ttttattcat aaactggcgg
    16501 atggttctac tcgtcatgtg cagacctatg ccggaccgat tgaaatttat ggcgacaagc
    16561 tcatgttatg tattgtgcat gatattactg agcaaaaacg gctggaggag cagctggaac
    16621 atgctgctca ccatgacgcg atgaccggat tactgaatcg gcgacagttt tatcacatta
    16681 cggaaccagg ccaaatgcag catctcgcca tcgctcagga ttacagcttg ttgctcatcg
    16741 acaccgatcg ttttaaacac attaacgatc tctatgggca ttctaaaggt gatgaggtgt
    16801 tatgcgccct cgcccgcccc ctcgaaagtt gcgctcgcaa aggcgatttg gtgtttcgtt
    16861 ggggaggcga agagtttgtc ttattgctac caagaacccc actggatacc gcgctttcgc
    16921 tggctgaaac tatccgcgta agcgtggcaa aagtgagtat ttcgggctta ccacgcttta
    16981 ccgtcagcat tggtgtggcg catcacgaag gaaatgaaag catcgatgaa ctgtttaaac
    17041 gcgttgatga tgctttgtat cgggcgaaaa atgatggacg caaccgcgtg ctggcggcat
    17101 aagccgcgga tgcgtctcga gatcaacgac tgcgcttagc gtggcgctcc cagttttctt
    17161 gcttcgcctg cgccgtttta cgtagtgcga cgtaacacgc cccgctgccg ccatgatgcg
    17221 gtagcgcggt gcaatatgcc tgaacatcat caaattcggt cagccagcgc gccacatagc
    17281 tgcggacaat attggcatgt gatttatctt cccgcccttt accatgaata atcagcacat
    17341 tacgcaaacc atccgccagg gcttgttgaa tgaaactgaa caccattttg cggcactctt
    17401 ccaccggctg gcgcaaaaga ttcaggctcg cctgttgcgg atatttacca ctgcgcagct
    17461 tatccagcac cccatgctgc aacccttcct gccgaaactc cagcggctga cttagcggga
    17521 tgatgtcgag aaatccggta gtgaggaaat tatcaagctg cagcgtgtcg atacgctgcg
    17581 gcgcacgttg gttacgcgtt ggatgccagt ggacatctgt agcacgtttc agcggctgga
    17641 catcttccat ggcgtcaaga aacagcgatt tgtcgtcaag gttcatgtaa cctcctatgc
    17701 accttcacac gtcgtgattc agacgggtta attgttcata cttactgcat cgttcccttc
    17761 acagattgaa acaatactct acttcatacc tgtgagtatc tgcgcaggcg gggcatttta
    17821 tactagcctg ttagcagtat cgaacagttt agcccgagtt taattcagca caggctccga
    17881 tcaggatgat ttattcatag caggaaccaa caaaaatgcg ataaataaca gaccactgat
    17941 tagcagaaag gtaccgaaaa tgccatagtt tgcgcctccc tcagcatcaa ttgactcaat
    18001 aatttggttg aagccagtat ttccgatgtc tggtcacttc tactgcgatt aaaaaataaa
    18061 gcacttctgg aaatgaagtc attcaccgta tggggaacag aaaccatttt ttcacccatt
    18121 ctcgaaaaat gaaaattagt tcgattattg agaatagagc cacacagggc aataccaatc
    18181 gcgcccccaa gattacgtgt taaattaaat acaccagagg ctaacttcag ccgttctttt
    18241 ggaattcccc ccagcgttaa tgttacgatg ggggccatcg caaactgctg caaaatccct
    18301 ctaattgcct gtggaaacag taattcctgc catccccatt cgtgggttat aggggtaaaa
    18361 agatacattg aaaacacaaa tccgcccagc cccgccatca acagccatcg cagatttatt
    18421 tttttactta accagaagta aaagggaaca gaaaataact ggaaaatacc tgttgtgcaa
    18481 acggcaaaac ctatctcttc ggcatttaat ccgcgaatct gccccaaaaa cacaggtatt
    18541 aaatatacag tagagaatat cccaacccct ccagaaaaag aaaaaaaaca tcccagagtg
    18601 aaatttttat ccttaaaagc atgaagatcc attatcggat tggatattgt aagtgtacgc
    18661 gcagcaaata aaataaaact caccaatgcc agaactgatg tcagcaaaat agtattatca
    18721 tccagccatc cccagcgcgc tccttcttca agggtatatt ccagacatcc cagagtcatg
    18781 gccaacagga taatactcgg ataatcagca actttcagta aagagagatc aggtttatca
    18841 aaattaacca gaaaaggaat actcaaaact aaatatatgc caggtaatat attgatataa
    18901 aatagccatc gccagtcgag gttatcagtg agccagcccc ccagagttgg ccctagcgtc
    18961 ggtgacagtg aggccagcgc actgactacc gcagcagcca accccaactc tttaccctgg
    19021 taataaataa acgccgtcgt gaaaactaaa gggatcatgg atgcgccagc agctccctgt
    19081 aaagcacgaa acaaaatcat gatctgaata ttccatgcaa gtccacacgc gatactcatt
    19141 aaagtaaata ttcccgcgga tagagtaaaa agccaacgag tggaaaatac ccgtgacagc
    19201 cacccagaga gcggaataac aataatctca gcaattaaat agctggtctg aagccaggcc
    19261 atttcgtctt tacctgcgga cagaccacca cctatttcat ttagggatga cgatacaatt
    19321 tgaatatcaa gctgagcaat aaataatccc atacacatac tggcgaaagc aatatgttta
    19381 cgcatagaca tcatctctca tcaacactga cggtgacgga cagtcccggg cgaagaagtt
    19441 gctttaattc ctttgcgtcg tcaaatgcaa tccgtaccgg gacacgctga acaattttgg
    19501 tgaaattgcc agtggcattt tctatcggaa tcaagctgaa actggctcct gtcgctgggg
    19561 aaaggctttc aatgtgaccg tgaaacactt cccctttgag aatatcagca cggatctcag
    19621 ccttcattcc tggcttcatt cccgctattt ggttttcttt ataatttgca tcaacccaca
    19681 actcactgac tggtactagt gaaactaacg atgtgccgcc ttccacccaa ctgcctgtat
    19741 gggcgctgcg gtttgcgaca atgccatcaa tcggcgca
//
LOCUS       DAJTYG010000072        19377 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTYG010000072 DAJTYG010000000
VERSION     DAJTYG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.072"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="OO568_002727"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="OO568_002727"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6642022.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="OO568_002728"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="OO568_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6642023.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3744..4163)
                     /gene="atpC"
                     /locus_tag="OO568_002729"
     CDS             complement(3744..4163)
                     /gene="atpC"
                     /locus_tag="OO568_002729"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6642024.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4184..5566)
                     /gene="atpD"
                     /locus_tag="OO568_002730"
     CDS             complement(4184..5566)
                     /gene="atpD"
                     /locus_tag="OO568_002730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6642025.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5593..6456)
                     /gene="atpG"
                     /locus_tag="OO568_002731"
     CDS             complement(5593..6456)
                     /gene="atpG"
                     /locus_tag="OO568_002731"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6642026.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6507..8048)
                     /gene="atpA"
                     /locus_tag="OO568_002732"
     CDS             complement(6507..8048)
                     /gene="atpA"
                     /locus_tag="OO568_002732"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6642027.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8061..8594)
                     /gene="atpH"
                     /locus_tag="OO568_002733"
     CDS             complement(8061..8594)
                     /gene="atpH"
                     /locus_tag="OO568_002733"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6642028.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8609..9079)
                     /gene="atpF"
                     /locus_tag="OO568_002734"
     CDS             complement(8609..9079)
                     /gene="atpF"
                     /locus_tag="OO568_002734"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6642029.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKLRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9141..9380)
                     /gene="atpE"
                     /locus_tag="OO568_002735"
     CDS             complement(9141..9380)
                     /gene="atpE"
                     /locus_tag="OO568_002735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6642030.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9427..10242)
                     /gene="atpB"
                     /locus_tag="OO568_002736"
     CDS             complement(9427..10242)
                     /gene="atpB"
                     /locus_tag="OO568_002736"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6642031.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10251..10631)
                     /gene="atpI"
                     /locus_tag="OO568_002737"
     CDS             complement(10251..10631)
                     /gene="atpI"
                     /locus_tag="OO568_002737"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6642032.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11248..11871)
                     /gene="rsmG"
                     /locus_tag="OO568_002738"
     CDS             complement(11248..11871)
                     /gene="rsmG"
                     /locus_tag="OO568_002738"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6642033.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11935..13824)
                     /gene="mnmG"
                     /locus_tag="OO568_002739"
     CDS             complement(11935..13824)
                     /gene="mnmG"
                     /locus_tag="OO568_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6642034.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14203..14646)
                     /gene="mioC"
                     /locus_tag="OO568_002740"
     CDS             complement(14203..14646)
                     /gene="mioC"
                     /locus_tag="OO568_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6642035.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14736..15194)
                     /gene="asnC"
                     /locus_tag="OO568_002741"
     CDS             complement(14736..15194)
                     /gene="asnC"
                     /locus_tag="OO568_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6642036.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15346..16338
                     /gene="asnA"
                     /locus_tag="OO568_002742"
     CDS             15346..16338
                     /gene="asnA"
                     /locus_tag="OO568_002742"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6642037.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPVAVRESVPSLL"
     gene            complement(16343..17794)
                     /gene="viaA"
                     /locus_tag="OO568_002743"
                     /pseudo
     CDS             complement(16343..17794)
                     /gene="viaA"
                     /locus_tag="OO568_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(17788..19284)
                     /gene="ravA"
                     /locus_tag="OO568_002744"
     CDS             complement(17788..19284)
                     /gene="ravA"
                     /locus_tag="OO568_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6642038.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLKASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4627 a         5120 c         4859 g         4771 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca attgaccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc aacagttcgt tgttcggtgc
      301 aacgacgata accggcatat ccgcgtcaat cagcgccagc gggccgtgtt tcagctcgcc
      361 tgcagcgtag gcttcagcgt gaatgtaaga gatctctttc agcttcaatg cgccttccag
      421 cgcgattggg tactgatcgc cacggcccag gaacagcgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttatcctg agacagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttta tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaggccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt ttcagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccattatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatgca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcgag tcatatgacc
     1741 ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ctaggtaagt cagatgcccg gctttcgtgc ctttacccag acgcgctttt
     2341 ttcatctcaa cgaagttacc aacgtgtgca ccttccagca actcagcacc aggacgcaga
     2401 cgggcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttggc aatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctggggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcaacgc ctgtttaagc agatcgccgc cgtgaccgta caccaggtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg ggtcaacgcg taaaaaaaag ccagcctgtt tccagactgg
     3721 cttttgtgct tttcaagccg gtgttacatc gcttttttgg tcaactcgat aacgcgcagc
     3781 tgcgcgatcg ctttggccag ttccgcagac gcctgagcgt aatctacgtc gccgtgagag
     3841 ctgctaatgt gctcttcagc cttacgtttc gcttccatgg ctcgcgcttc gtcgagatcc
     3901 tggccgcgaa ttgcggtgtc ggccagaacg gtcacgttgc caggctgcac ttcaagaatg
     3961 ccgccagaca gatagataaa ctcttcgtga ccgtgctgtt tcacgatgcg aatcatacca
     4021 ggcttaatgg cggtgagcag cggtgcgtgg ccagggtaga tccccagttc accttcgcta
     4081 cccgttacct ggattttctc gaccagacca gagaacattt gttgctctgc gctgacgacg
     4141 tccaggtggt aagtcattgc catatcaccc tccgattaag gcgttaaagt tttttggctt
     4201 tttccacagc ttcttcgatg gaaccgacca tgtagaacgc ctgctccggc aggtgatcgt
     4261 attcgccttc catgatgcct ttaaagccac ggatggtgtc tttcagggag acgtatttac
     4321 ccggagaacc ggtgaatact tctgccacga agaacggctg ggacaggaag cgctggatct
     4381 tacgagcacg cgctaccacc agtttgtctt cttcagacag ttcatccata cccaggatgg
     4441 cgatgatgtc tttcagttcc tgataacgtt gcaggatgga ctgaacgcca cgcgcggtgt
     4501 cgtagtgttc ctgaccaacc accagcgggt ccagctgacg gctggtggag tccagcgggt
     4561 caacggccgg gtagataccc agagacgcga tctgacggct cagtaccacg gttgcgtcaa
     4621 ggtgcgcaaa ggtggttgcc ggagacgggt cagtcaagtc atccgcaggt acgtataccg
     4681 cctgtacgga ggtgatagaa ccagttttgg tggaggtgat acgttcctgc agaacgccca
     4741 tctcttccgc cagggtcggc tgataaccta ccgctgaagg catacggccc agcagtgcgg
     4801 atacttccgt accggccagg gtgtaacgat agatgttgtc aacgaacagc agaacgtcac
     4861 gaccttcgtc acggaatttc tcagccatgg tcagaccggt cagagcaacg cgcagacggt
     4921 ttcccggcgg ctcgttcatc tggccataca ccagggatac tttgtcgata acgttggagt
     4981 cggtcatttc gtggtagaag tcgttaccct cacgagtacg ttcacctacg cccgcaaaca
     5041 cagagtaacc ggagtgctcg atcgcgatgt tacgaatgag ctccatcatg tttacggttt
     5101 tacctacacc cgcaccaccg aacagaccaa ctttaccgcc cttagcgaac ggacacatca
     5161 ggtcgataac tttgataccg gtttccagca gttcctgaga gtttgacagc tcttcgtagg
     5221 aaggtgctgc gcggtgaatc gcccaacgct cttcttcacc gatctcgcct ttcatgtcga
     5281 ccggttcacc cagtacgttc atgatacggc ccagagtcgc tttacctacc gggacttcga
     5341 tcgggtgttc gaggtctttt acatccagac cgcgacgcag accgtcggag gaacccattg
     5401 cgatggtacg cacgataccg ccgccgagct gctgctgaac ttccagcacc agacgctcat
     5461 taccattttg cacctcaaga gcatcgtaca cgcgcggtac ggcatcctga gggaattcga
     5521 cgtcaactac ggcgccgatt acctggacaa tctttccagt agccatctta aatcctctac
     5581 gaaataacct gtttaaaccg cggcggcccc cgagacgatc tcggtgagtt cctgagtaat
     5641 gctggcctga cgagctttgt tgtataccaa ctgcagctct ttaatcaggc tgccgccatt
     5701 gtcggtcgcg gctttcatcg ccaccatacg ggcggcctgc tcgctggcca ggttttcaac
     5761 cacgccctga taaacctgag attcgacata acgacgcagc agggtatcca gcaacgcctt
     5821 cggatcgggt tcgtacaggt aatcccagga tttatgtttc agatcatcat catctgatgc
     5881 cggtaacggc agcagctggc tgatggtcgg aacctgagac atggtgttaa taaatttgtt
     5941 gctgacaatg taaagcttgt ccagacggcc ttcgtcgtag gcctgcaaca tcacttttac
     6001 cggaccgatc agttcggaca gggaagggtt atcccccatg ccggtgactt gggcaacaac
     6061 attgccgccc acggagttga agaacgacac gcctttcgag ccgatcattg cgaggtcgca
     6121 ttgaacgcct ttgtcggtcc aggtcttcat ttccgccagc agttttttga acaggttaat
     6181 gttcaaacca ccgcacaaac cacggtcggt cgacaccacc aggtagccca cgcgtttaac
     6241 gtcgcggtct tccaggtaag ggtgcttata ttccagatta ccgtgtgcaa ggtgaccaat
     6301 cactttgcgc atggtttctg cataaggacg gctggccgcc atgcgatcct gcgatttacg
     6361 cattttggaa gcggcgacca tctccatcgc tttagtgatc ttttgcgtgt tctggacgct
     6421 tgcgatctta ctacgtatct cttttgcgcc ggccatgagc ttctcctcaa tgccttgcgg
     6481 cctgccctaa ggcaagccgc cagacgttac caggattggg ttgctttgaa ggaatcgagg
     6541 atgcctttca gcttgccttc gatttcgtcg ttgtagccac cggtctggtt gatctcttgc
     6601 atcaacggag cgtgatcacg gtcgacgtaa gccagcagag cggcttcgaa gctgccaatt
     6661 ttcgacagtt caacatccgc caggtaacca cgttctgctg cgaacagaac cagagactgc
     6721 tgcgcaacgg acatcggcgc atactgtttc tgtttcagca gttcggtcac tttctgaccg
     6781 tggtcaagct gcttacgtgt tgcatcgtca aggtcggatg caaactgaga gaacgctgcc
     6841 agttcacgat actgtgccag agcggtacgg ataccaccgg acagtttttt catgatcttg
     6901 gtctgtgctg caccaccaac acgggatacg gaaatacccg ggttaaccgc aggacgaata
     6961 ccggcgttga acaggttggt ttccaggaag atctgaccat cggtaatgga gattacgttg
     7021 gtcggaacga acgcagaaac gtcacccgcc tgagtttcga taatcggcag tgcggtcaga
     7081 gaaccggttt tccctttcac ttcacctttg gtgaaggctt caacgtattc ggcgttaaca
     7141 cgtgcagcac gctccagcag acgagagtgg aggtagaaaa cgtcgcccgg gaatgcttca
     7201 cgtcctggcg gacgacggag cagcagggag atctgacggt aagcaacagc ctgtttagac
     7261 aggtcatcgt aaatgatcag cgcatcttca ccgcggtcac ggaagtattc gcccattgcg
     7321 caaccggcat acggtgccag gtattgcagt gcagcggatt cagacgcggt tgctaccaca
     7381 acgatggtgt tagccagtgc gccgtgctct tccagtttac gtaccacgtt agaaatggtg
     7441 gacgctttct ggccgatagc gacatagata catttgatac cggaatcgcg ctggttgatg
     7501 atggcatcga tagccagggc ggttttacct gtctgacggt caccgatgat caattcacgc
     7561 tgaccacgac cgattgggat catggagtca acggctttat aaccggtctg taccggctga
     7621 tctacggact gacgttcgat aacgcccgga gcgattgctt ctacagcaga gaagccgtcg
     7681 tgatccagcg gacctttacc gtcgattggt gcacccagag tgttaaccac acggcccagc
     7741 aggccacggc caaccggaac ttccaggata cggccagtac acttaacttt catgccttcg
     7801 gcaaggtcag cgtacggacc cataacaacc gcacctacag agtcgcgctc gaggttcagt
     7861 gcgatagcgt aacggttacc cggcagggag atcatttcac cctgcataca atcggccagg
     7921 ccgtgaatgc ggataacacc gtcacttaca gaaacaatag taccttcgtt gtgagcttca
     7981 ctcacaacat tgaactgagc aatgcgctgc ttgatcagtt cgctgatttc ggtggaattc
     8041 agttgcatgc tccagtcccc ttaagactgc aagacgtctg caaggcgctc aagacgaccg
     8101 cgtacgctgc catcaatgac catatcaccc gctcggatga taacgcctgc cattacagac
     8161 ttatcgattt tgcaattcag cttaactttg cgtgacagac gtttttccat cgcagcagaa
     8221 attttcgcga gctgttgttc actcagtgcg gcagcggaaa tgacgtctac ctcagcggta
     8281 gcctcactca cggcacgaag gtgaataaac tgctccagaa catccgggag cgcgttaaga
     8341 cgaccatttt cagccattac ccgaatcagg ttctgaccgt tttcgtccag ttgctcacca
     8401 caaactgcga taaacgactc ggcgagcgtt tctggcgcaa gcgcgccaga gagaagctct
     8461 gccatttgtt cgtttttggt tacctcggcg gcaaacgcca gcatgtcctg ccagcgttct
     8521 acactttggt gttcgacggc aaagtcaaaa gctgctttgg cgtaggggcg agctaccgta
     8581 ataaattcag acatcagccc ctccctcctt acagttcagc gacaagttta tccacgatgt
     8641 cgctgttagc agcttcatcc acggaacgtt cgatgatctt ctcggcgcca gcaacagcca
     8701 ggatagcaac ttgcttacgc agctcttcac gggcacgttt acgctcggct tcaatttctg
     8761 cctgcgcctg ggccacgatt ttagtacgtt cctgttctgc ctcagctttc gcttcgtcca
     8821 gaatctgcga gcggagttta ttcgcctgct cgatgattac ctgggcttcc gctttcgctt
     8881 ttttcagctg gtcggtcgcg ctggcctttg caaggtcaag gtccttatgt gctcgttctg
     8941 cggaagcaag gccgtcagca atttcttttt gacgtttttc gatggctgcc attaatggcg
     9001 gccatacgta cttcatgcag aacagaacga acaggacaaa cgcgatggcc tggccgagga
     9061 ttgttgcgtt aagattcaca gcacaatgcc tctatttagt taacgttctg atattgctct
     9121 ttaaataaaa gcaacgctta ctacgcgaca gcgaacatca cgtacagacc cagacctaca
     9181 gcgatcatcg ggatagcatc caccagaccc ataacgataa agaactgagt acgcagcaga
     9241 ggaatcagat caggttgacg cgctgcgcct tccaggaatt tacccccgag gatgccgata
     9301 ccgatcgcag caccgattgc cgccagaccc atcatcacag cggcagccat gtacagcaga
     9361 tccatattca ggttttccat gacagtctcc agtttgtttc agttaaaacg tagtagtgtt
     9421 ggtaaattaa tgttcttcag acgccatcga cagatagacg atcgtcagaa ccatgaagat
     9481 gaaggcttgc agcgtaatga tcaggatgtg gaaaatggcc cacggcacat tcaggatcca
     9541 ctgtgaccac cacggcaaca gaccagcaat cagaatgaaa atcagctcac cggcatacat
     9601 gttaccgaac agtcgcaaac cgagtgaaac tggtttggac agcaggctta ccccttcaag
     9661 gattaagttg acaggaatga acgcccagtg attgaacggc tgcagcgtca actctttcgt
     9721 gaagccgccg atgcctttca ttttgatgtt gtagaacaga atcaggataa atacgcccag
     9781 tgccatagac agcgttacgt tcacgtccgc agacggaacc acacgcagtg caggcagacc
     9841 cagtacatgt tcagcaatgt acggcagcaa gtcgataggc agtaaatcca tcagattcat
     9901 caggaatacc cagacgaaga tcgtcagggc cagcggagca atcagcttgc ttttgccatg
     9961 gtacatgtct ttcacgctac cattaacaaa gccgatcacc agctcaatcg cggtctgaaa
    10021 cttacctggc acaccgctgg tcgccttttt ggctacgcta cggaataaaa ccaagaacaa
    10081 cagacccagc accaccgaga agaacatgga gtcaatattg attgtccaga aggtggctgg
    10141 ggggttttgt ggatccacca gcgagaatgt acgcaggtcc agctgaaggt tattcaggtg
    10201 gtgtcctatg taatcctgcg gcgtcatatt ttctgaagcc atgatgcctt ttaccctttg
    10261 ttgttaatta cagccggtgc cagtatctga accaccagca ccaaaaccca cgtaacgatc
    10321 agcggcaaga ataccgcctt taaaaccgcc aacgccacca ccagtaacac caacatcgcc
    10381 agaactttga aagcttcgcc aaatgcgaat gtccaagcca cccggccttt cgctggtgta
    10441 tgcgcctggt gacgccaggc aaatatcata aacaaaacgt taggcagaaa gactgccagg
    10501 cccccgctta ttgcagagac gccccagaag gggtctttga ggctgaacag caatccactt
    10561 gctatcacca ccagtaactg aacgagcaga agcttccgag caacgtttcg actcacgagc
    10621 gacacagaca tcacgttttt cactcctgct cccttcgagg tatgccgcgt gtcgtataaa
    10681 actttcttta aggcttagag tcaagcatca aaaagcggtc aaattatacg gtgcgccccc
    10741 gtgatttcaa acaataagta gccaaaaggt gaataaatgt ttaaatattt ttccagtgca
    10801 tacaattgcg acttttccgc tacccctgtt cgatcatgaa aaactgtaaa taacgcgtaa
    10861 acactggtga taaagcgtgc ttcagatcac atattgcgca tgttcgcgca cagcatattt
    10921 atttacttgg caaatgatgc ctttgcaagt ttatgatatt tcagtctaaa aacagatact
    10981 gttttaataa atgacattta cacaacaaaa accacccatt gacattttta ataatgtttt
    11041 aacagccaat gatggttctt agcgccgatt tttagcagac tgatattttc actaatgact
    11101 tattttctgc ttaccaaaaa aagccacgtt atcttgttga tgcaaaagag tgaacgtggc
    11161 gttaaatgta accagttata tcagtagaaa acctggttgt tgttaacagt ctaaccggtc
    11221 aattttttat gatttttttg ataaaaatta aattttattt gctttaatca ccaccagatg
    11281 acgttcgcca tccagggctg gaacctgaag tttaaccact gattcgacct gatattcttc
    11341 gggcaacaaa gcgatttcat cttccggcat ttgccctttc agcgcgtaga aacggccttg
    11401 ctcaccagga agatggtggc accagctcac catatcgttc agagaggcaa aagcgcggct
    11461 aattacgcca tcaaatggcg gctctgaagg aaactcttct accctgctct gtactggttc
    11521 aatattctcc agtttaagct catgttgcac ctgacgaagg aaacgcacgc gtttaccaag
    11581 gctatccaac agagtgaaat gggcttcagg acgcacgata gagagtggaa tgcctggcag
    11641 tcctggtccg gtgccgacat cgataaaccg ttcaccttgc agatacggtg ccaccacaat
    11701 gctatcgaga atatggcgta ccagcatctc attaggatcg cggaccgaag tcaggttgta
    11761 cgctttgttc catttatgca gcatattcac gtaggcaata agctggtttt tctggtgatc
    11821 agtaagcgaa atacctgcgt ctttcagcag taaggagagt ttgttgagca cggtgattac
    11881 ctgttcttga tgcgttgcct ggtaagcggg tgcttaccag gcatttttaa tgcgttatgc
    11941 gctacgacgc agcataccct gttttttcag ccacaccagc agaatggaga tggccgcagg
    12001 cgtgacgcca gaaatacgcg aagcctggcc gatagaggcc ggtttgtgat cgttaagttt
    12061 ggcgatcact tcgttagaaa gaccggatac ctggcggtaa tccagtgtcg cgggtagcag
    12121 ggtgttctcg ttacgcagct gcttttcgat ctcatcttgc tggcgcgcga tataaccttc
    12181 gtatttaacc tgaatctcaa cctgttccgc cgcctgttcg tctgtcaacg caggggcaaa
    12241 cggcgtcagc gtggttaatt tttcataagt catttccgga cgacgcagca gatcttcacc
    12301 actggcttca cgggaaagcg gcgcagtcag gtgagcattc acttcggctg cagcttccgc
    12361 cgacggggtt acccaggtcg atttcagacg ctgacgctca cgctcgatat tctcaagttt
    12421 ctcgttaaag cgcgcccaac gttcgtcatc caccaggccc agttcacgac cgatttcagt
    12481 caaacgcaga tccgcattat cttcgcgcag catcagacga tattctgcgc gcgaagtaaa
    12541 catacgatac ggttctttgg ttcctaaagt gcacaggtca tcaaccagta cgccgagata
    12601 cgcctgagaa cgtgccggag cccaaccttc tttgtcagca gacagacggg cagcgttaag
    12661 accggccagc aagccttgcg cagcggcttc ttcgtaaccg gtagtgccgt taatctgacc
    12721 agcaaagaac agcccctgga taaacttgct ctccagcgtc ggtttcaggt cgcgaggatc
    12781 gaagaagtca tactcaatgg cataacccgg acgcacgatc ttcgcgtttt ccatcccctg
    12841 catagagcgg acgatttgca tctgcacatc gaacggcagg ctggtggaga taccgttcgg
    12901 ataaatttca ttagaggtca gtccttccgg ttcaaggaag atctgatgct gatttctgtc
    12961 tgcgaagcgc atgactttgt cttcgatcga cgggcagtag cgtgggccga caccttcgat
    13021 cacccctgca tacattgggc tacgatcgag gttactgcgg atcacatcat gggttttctc
    13081 gttggtatga gtgatataac acggcacctg ctggggatgc agggacgcat tgcccataaa
    13141 cgagaatacc ggcattgggt tatcgccatg ctgttgcgcc agcacgctaa agtcgatggt
    13201 tcgagcatca atacgcggcg gagtaccggt tttcagacga ccaacgcgca gcggcagttc
    13261 acgcaaacgg cgagagagcg gaatggatgg cggatcgcca gcacggccac cgctgtaatt
    13321 atccagaccg atatgaattt tgccgtcgag gaacgtccca acggtgagca ccacagcttt
    13381 ggcacggaac ttcagtccca tttgggtaac agcaccgacc acgcgatcgt tttcgacaat
    13441 aagatcttca accgcctgct ggaagatcat caggttcggt tggttctcca gcgccgtacg
    13501 taccgcctga cggtagcgca cacgatccgc ctgagctcgg gtagcgcgaa ccgccggtcc
    13561 tttgcttgcg tttagtatcc taaactggat acccgcttga tcgatcgctt tcgccatcag
    13621 accgccgagt gcatccactt cttttaccag atgtcccttc ccaataccgc cgatcgccgg
    13681 gttgcagctc atctgcccca gagtgtcgat attgtgtgtc aaaagcagag tctgttgacc
    13741 catacgcgcc gcagccatcg cggcctcggt gcctgcatga cccccgccaa tgatgatgac
    13801 gtcaaaagga tccggataaa acatggtgat tgcctcgcat aacgcggtat gaaaatggat
    13861 tgaagcccgg gccgtggatt ctactcaact ttgtcggctt gagaaagacc tgggatcctg
    13921 ggtattaaaa agaagatcta tttatttaga gatctgttct attgtgatct cttattagga
    13981 tcgcactgcc ctgtggataa caaggatccg acttttaaga tcaacaacct ggaaaggatc
    14041 attaactgtg aatgatcggt gatcctggac cgtataagct gggatcagaa tgaggggtta
    14101 tacacaactc aaaaactgaa caacggttgt tctttggata actaccggtt gatccaagct
    14161 tcctgacaga gttatccaca gtagatcgca cgatctgtat acttatttga gtaaattaat
    14221 ccacgatccc agccattctt ctgccggatc ttccggaatg tcgtgatcaa gaatgttgat
    14281 cttcagtgtt tcgcctgtct gttttgcacc ggaatttttg agttctgcct cgagtttatc
    14341 gatagcccca caaaaggtgt catattcacg actgccaata ccgattgcgc caaagcggac
    14401 cgcagaaaga tcgggcttct gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg
    14461 aatatctccg gcaccgtggg tggagctgat aaccagccag atccccgagg cagataaatc
    14521 ttccaacagc ggaccgtgca gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc
    14581 caggtgttct gctacatatt cggcaccgcc gagggtgctg ccgctgataa gagtgatatc
    14641 tgccataaac cgccaccttt attaagagtg gcgtattgta cgctgtgaac tcgttgggat
    14701 ctacctgtgg aaaagtatgg gattaaaaaa gccgatcagg gcttgatggt acgcatgatt
    14761 gggttttgca ggacgatcaa tgtctcggtg gactgaattt catcaattgt ttggatcttg
    14821 ttgataagta catgctggag agcgtcgatc gaacggcaca taacttttat aaagatgctg
    14881 tagtggccgg ttgtgtaata ggcttcagtg acttcatcaa ggctttccag ctttgccagc
    14941 gcggaagggt agtctttggc gctctttaat ataatgccga taaagcagcc tacgtcataa
    15001 ccgagctgct tcgggctgac atcaatacgc gccccggtaa tgatccccgc ctgcttcatt
    15061 ttctctactc gaacgtgaat cgtccccgga ctgacgccaa attgtttcgc cagttcggcg
    15121 taagcggtgc gcgcattgcc cattaatgct tccaggatgc cacggtccag attgtcgatc
    15181 agataatttt ccataggatt ttcttatgca gattgatgat tcattctatt ttagccttct
    15241 tttttaatga atcaaaagtg tgttaggctt tttattgaat gattattgca tgtgtgtcgg
    15301 tttttgttgc ttaatcataa gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac
    15361 attgccaaac aacgccaaat tagcttcgtg aaatctcact tttctcgcca actggaagaa
    15421 cgtctggggc tgatcgaagt ccaggcgccg attcttagcc gtgtggggga tggcacgcag
    15481 gataacttgt cgggctgtga aaaagcggtg caggtaaaag tgaaagctct gcctgatgcc
    15541 cagttcgaag tggttcattc actggcgaag tggaaacgtc agaccttagg gcaacacgac
    15601 ttcagcgcgg gcgaagggct gtacacgcac atgaaagccc ttcgccccga tgaagaccgt
    15661 ctttctccgt tgcactcggt ctatgttgac cagtgggact gggaacgcgt aatgggcgac
    15721 ggtgagcgtc aattctcgac tctgaaaagc acggtagagg cgatctgggc gggaattaaa
    15781 gcaaccgaag ctgcggttaa cgaagagttt ggcctggcac cgttcctgcc ggatcagatt
    15841 cacttcgtac acagccagga gttactgtct cgttatccgg atctcgatgc caaagggcgt
    15901 gagcgggcga ttgcgaaaga tcttggcgcg gtattcctcg tcgggattgg cggcaagttg
    15961 agtgatggtc atcgtcacga cgtgcgcgca ccggattatg atgactggag caccccgtca
    16021 gagctgggcc atgcgggcct gaacggcgat attctggtgt ggaacccggt actggaagat
    16081 gcgtttgagc tttcttccat ggggatccgc gtggatgccg acacgctgaa gcatcagctg
    16141 gcgctgaccg gtgatgaaga tcgtctgcag ctggaatggc atcaggcgct gctgcgcggt
    16201 gaaatgccgc agaccatcgg cggcggtatc ggccagtctc gtttgactat gctgctgctg
    16261 caactgccgc atatcggcca ggttcagtgt ggagtatggc cagttgctgt tcgcgagagc
    16321 gtcccttctc tgctgtaata atttatcgcc gccagcgtct gagcaggcgg cttcgcatcc
    16381 cggtatcaaa gcgccagata tgatcgaaaa tgcgcatgat gccgggtttg ccgtgtgccg
    16441 acatcgccac ggcatgaaag cgatgctgat gtacccgctg cagctctttc actttactcg
    16501 tcacgtcgtc aggcaaccgc tgagcgataa aatcagaaat caccaccgca tcggcatcaa
    16561 accattccct gctttgcaag cgttccataa tggcgcgaaa acaactggca agatcggtgc
    16621 cgctacgaaa ctgctggctt aaaaaacgga ttgcttgttc gatgccttgt gggcctgaaa
    16681 gctcataacg gacgatctcg gtggaaaata gcataatata acagcgccgg ttttcagcga
    16741 gagcaatgcg catcaaagcc aggcagaacg ctttcgcaca ctgttcatta aagccgccca
    16801 ttgagccgga agtatccaca cagacaataa acggcccgcg cggctgttca tcgtaatctt
    16861 tatgtaccac cggacgttcg agcacttttt cacgccacga ctcaccgtgc aggcgatagg
    16921 tgagcaactg tttttccacc agccgacggt aaaactcata ctccagttcc gttatcccta
    16981 gtgtcgccag ttctggcggc aggagacgta aaatatcatc gctttgttgc agaccatcaa
    17041 cctgctcagg aaccgtcgcc ggttcgcgca ccatggtgcg gaaggtttcc atctacgcat
    17101 cgttgcgcgg tattgatttg gcttcccgag aacgccccag ctgctctgcc aggcgtttca
    17161 gttccggctg ttcgttaaga aattcaccgt atttcacaat aaactgatag tcgccacgtt
    17221 taagctgacc ggcgctcata tcccacagac gaccagctgc ggtattgtta tctgcgagaa
    17281 tcggttcaag ttgtccgctc agcgtcatgc gttcctgaac ttcacttaac agttgttcgc
    17341 gttcttcttc taatagctgt tgatttaacg tcgttgcttg cacgatcaga cttaaacgcc
    17401 aacgctggag aaaaagcgtg tgtaacgctg aagttattgt gctgttagca tcaaccaact
    17461 gccgggcttg ttctgcccat ggggaattca gacgatgcag taagtctagg atctgtggta
    17521 gctgcacaat aaactgcggc gtggagagga gctggctttg ctgatagcac atcacctctt
    17581 cggtgagttc cggcgggact cgggcatctt tcagccgact gcgcagcgcc tcacgccagc
    17641 ggggaacatc atcagtgatt gccgccttca gtcgtgggaa tttttcaaag aagaccgcca
    17701 gctgcggtga ggccagcagt gcgatgatca tctcttcgat caatccctct tcgctgacgg
    17761 ccagcatcac attaagcgta tccagcgtta gcattgttgt gcctggcgaa tctgtgcacc
    17821 gacatcctgt aggctggctt caatgcgacc taaccagtcg ccagggataa acagacattt
    17881 ttgctgttcg ctgaataaag cgtgttgctt acgccagtcg ctttcaagct cttccagttg
    17941 ttgttttatt tcacctggca gaccttcagc cgatgaaccg ggaagtgcca gcgtactgcc
    18001 ttgtaaactt acatcgcgta caacaagatg ttgggtgcta tcaacttcca gattcagttt
    18061 ctgggcaaag ccgataccgt tcagtttgcc gcgaatttca ccacctttac tcagccactg
    18121 ttccagcgcg ctacgctcaa aggagatatg aaccacttcc atatcatgca gttttaacgg
    18181 tttttgcagc agcagagtca gagtggaagc agtaacgtta acaggaagtt gatactgctg
    18241 acgacggctg aaaatgccgc ccagacgaat taacgttaag gctgttttat cgctttgttg
    18301 ctgctggagt tgcaggtgac gttgcacaat cgcgcccagg cgggtcaaca tcccttgctg
    18361 ttgccaggca tgaccggtca tcaatacatc aatttgttgt tgtatcaaat tcaggctttg
    18421 cgcgtcatac cacaggcaat ctttcagcaa aatgagatca accggggcaa cagcactgcg
    18481 accgctaaaa aaggcgctgg ccttcaataa gcggatcgct tttttccagc gacgatccga
    18541 gacataaggc gcatccggta atttatccag ttgctggcgc agcataaaaa tcagctcaaa
    18601 tacatgatcg ggcagcgtaa tttcaccaat ctctttctgc cagcgttcat attcttcatc
    18661 tgtgacctgc aaggcatcag gaaccggatt gtcgttttca tcctgttgac tggtcagcat
    18721 ggagcggaaa ttcgctttat cttgcacttt atctaaccat agacgaatca gcatgcggtc
    18781 atataacgct tccagactgc tgtctgcttc cggcagctcg ttggaggccg ccaccagtag
    18841 acgcatcggg attttttcta cgtgtgcacc gttgcggaac tggcgctcgt taatggcggt
    18901 gagcaaggta ttaagaattg ccgggcccgc tttccagatc tcatccagaa agacgatttc
    18961 tgcttccggc aggtaaccgc tggttaaacg ttcatagcgc ccttcatctt ttagcgcctg
    19021 aatagaaagg ggaccaaaaa cttcttccgg cgtggagaag cgggtcatca gatattcaaa
    19081 cgcgcgggca ttctgaaagg cgaattttaa gcgccgggcg atcaaacttt tggcaatacc
    19141 tggcgggcca aggaggaaca cactttcacc acttaatgcc gctaataaac acaagcggat
    19201 ggcgtggcta cgttcataaa gccccttttc caacgaactg ctcaggcggg aaattctttc
    19261 cgctaataaa tgagggtgag ccataatgaa gtggcgtcct ttcgtcaaaa gttctgcgta
    19321 aattgcgagt atagacgttt cttgctggtg gctaaaatag tctcaaaggg gggtaat
//
LOCUS       DAJTYG010000073        18175 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTYG010000073 DAJTYG010000000
VERSION     DAJTYG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18175
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.073"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..2083
                     /gene="pbpC"
                     /locus_tag="OO568_002745"
                     /pseudo
     CDS             1..2083
                     /gene="pbpC"
                     /locus_tag="OO568_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2233..2664
                     /gene="ndk"
                     /locus_tag="OO568_002746"
     CDS             2233..2664
                     /gene="ndk"
                     /locus_tag="OO568_002746"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6642039.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2814..3968
                     /gene="trmG/rlmN"
                     /locus_tag="OO568_002747"
     CDS             2814..3968
                     /gene="trmG/rlmN"
                     /locus_tag="OO568_002747"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6642040.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4253..5266
                     /gene="rodZ"
                     /locus_tag="OO568_002748"
     CDS             4253..5266
                     /gene="rodZ"
                     /locus_tag="OO568_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6642041.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5293..6411
                     /gene="ispG"
                     /locus_tag="OO568_002749"
                     /gene_synonym="gcpE"
     CDS             5293..6411
                     /gene="ispG"
                     /locus_tag="OO568_002749"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6642042.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            6522..7796
                     /gene="hisS"
                     /locus_tag="OO568_002750"
     CDS             6522..7796
                     /gene="hisS"
                     /locus_tag="OO568_002750"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6642043.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7814..8434
                     /gene="yfgM"
                     /locus_tag="OO568_002751"
     CDS             7814..8434
                     /gene="yfgM"
                     /locus_tag="OO568_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6642044.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8445..9623
                     /gene="bamB"
                     /locus_tag="OO568_002752"
     CDS             8445..9623
                     /gene="bamB"
                     /locus_tag="OO568_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6642045.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTTVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9741..11213
                     /gene="der"
                     /locus_tag="OO568_002753"
     CDS             9741..11213
                     /gene="der"
                     /locus_tag="OO568_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6642046.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11283..11498
                     /locus_tag="OO568_002754"
     CDS             11283..11498
                     /locus_tag="OO568_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6642047.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11495..12865)
                     /gene="xseA"
                     /locus_tag="OO568_002755"
     CDS             complement(11495..12865)
                     /gene="xseA"
                     /locus_tag="OO568_002755"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6642048.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWFFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13027..14493
                     /gene="guaB"
                     /locus_tag="OO568_002756"
     CDS             13027..14493
                     /gene="guaB"
                     /locus_tag="OO568_002756"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6642049.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGDIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14562..16139
                     /gene="guaA"
                     /locus_tag="OO568_002757"
     CDS             14562..16139
                     /gene="guaA"
                     /locus_tag="OO568_002757"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6642050.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEAQKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKTDLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16234..16773)
                     /locus_tag="OO568_002758"
     CDS             complement(16234..16773)
                     /locus_tag="OO568_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6642051.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16789..17307)
                     /locus_tag="OO568_002759"
     CDS             complement(16789..17307)
                     /locus_tag="OO568_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6642052.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17618..17809)
                     /locus_tag="OO568_002760"
     CDS             complement(17618..17809)
                     /locus_tag="OO568_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6642053.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWLHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17827..17979
                     /locus_tag="OO568_002761"
     CDS             17827..17979
                     /locus_tag="OO568_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642054.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
BASE COUNT         4300 a         4452 c         4994 g         4429 t
ORIGIN      
        1 gaagcgctga tcaattatga agatcgctgg ttctggaagc atccgggggt taatccattc
       61 tcggtggcgc gcgcagcatg gcaagatctc acttcgggac gggttatttc cggtggcagc
      121 acgctcacta tgcaggttgc tcgtctgctt gatcctcacc ccaaaacatt tggtggcaaa
      181 attctccagc tctggcgcgc gttgcaactg gagtggcatt tgtctaagcg tgaaattctg
      241 atcttgtatc ttaaccgcgc tccgtttggc ggtacgttgc aggggatcgg tacggcaagt
      301 tgggcttatc tcggaaaatc gcctgcgaat ttaagctatt ccgaggcggc aatgctggcg
      361 gtgttaccgc aagtgctcag ccgactgcgc ccggatcgtt ggccggagcg tgccgaagcc
      421 gcgcgtaata aagtgctcga acggatggct gcgcaaggtg tgtggtcccg tgagcaggta
      481 aaagagttaa gggaagaacc catctggctg gcaccccgac aaatgccgca actggcaccg
      541 ctgttttcgc gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac tacgctggat
      601 gccggtcttc aacgacgtct ggaagaactg gcgcaaaact ggaaagggcg gttgccaccg
      661 cgcagctcac tggcgatgat cgtggttaat cataccgata tgcgtgttcg cggctgggtg
      721 ggatcggttg atctcaacga tgattcacgc tttggtcatg ttgatatggt caatgcgatc
      781 cgatcgccag gatcggtgct caaaccgttt gtttatggtc tggcgctgga tgaaggcttg
      841 atccacccgg catcactgct gcaagacgtc ccccggcgca ccggtgatta tcgaccaggt
      901 aactttgata gcggttttca tggcccgatc aacatgagcg aggcgctggt gcgctcgctg
      961 aacttaccag ctgtgcaggt gctggaagcc tatggaccga aacggtttgc ggcaaagtta
     1021 cgcaatgttg gattgccgtt atatttgccc aacggcgctg cgccgaatct ttcactcatt
     1081 ctcggcggcg ctggtgcaaa actggaagat atggcggcag cgtataccgc gtttgctcgc
     1141 cacggcaagg caggcaaatt gcgcttacag cctgacgatc cgctgcttga acggccttta
     1201 atgtcgtcgg gggcggcgtg gatcattcgc cggattatgg ctgatgaagc gcaacccttg
     1261 ccggatagtg ccttgccgcg cgtcgcccca ctggcatgga aaacgggcac cagctatggc
     1321 tatcgtgacg cctgggcgat tggggttaac gctcgctatg tcattgggat ctggactggc
     1381 agacctgacg gcacgcccgt tgttggtcag tttggctttg ccagtgccgt accattagtt
     1441 gaatcaggtc aataatatct tactgtcgcg cagtgcgaat ctgccggaag acccgcgccc
     1501 gaactcagtc actcgtggcg ttatctgctg gccgggcgga cagtctttgc cggaaggtga
     1561 cggtaactgt cgccgccgcc tggcaacctg gctgctggat ggcagccagc cgccaacgct
     1621 gttactgtca gagcaggaag ggattaatgg cattcgcttc cccatctggc tggatgacaa
     1681 agggcagcgc gtcgccgctg acttcccgca ggcgaagcaa gaaatggtaa atgtctggcc
     1741 gctaccgctg gaaccctggc tgcccgcatc agaacgtcgc gctgtgcgct taccaccggc
     1801 ctcgacaatt tgcccgccat acggtcacga tgcccagctc cctctgcagc tgacaggcgt
     1861 tcgcgatggc gcgattatta aacgtttacc gggcgcagcg gaagcgactt tgccgttgca
     1921 atcaagtgga ggggcaggtg aacgctggtg gtttctgaat ggcgaaccgt taactgaacg
     1981 cgggcgcaac gtgaccctgc atttgacgga taaaggtgat tatcaattgc tggtgatgga
     2041 tgatgtgggg caaatcgcga cagtgaaatt tgtcatgcaa tagtctgttt tgtccctgat
     2101 tgttgctaaa actcatttta ttttaaaaaa aatgttacct tcatcaatag tcaacggccc
     2161 tgttgctcat tataatccgc gccatctcgt acgctggtac agacaacaac agaacaattt
     2221 acagaggtaa aaatggctat tgaacgtact ttttccatca tcaaaccgaa cgcggtagca
     2281 aaaaacgtca ttggtaatat ctttgcgcgc tttgaagctg cagggttcaa aattgtcggc
     2341 accaaaatgc tgcacctgac cgttgaacag gcacgtggct tttatgctga acacgatgga
     2401 aaaccgttct ttgatggtct ggttgaattc atgacctctg gcccgatcgt ggtttccgtg
     2461 ctggaaggtg aaaacgccgt tcagcgtcac cgcgatctgc tgggcgcgac caatccggca
     2521 aacgcactgg ctggtactct gcgcgctgat tacgctgaca gcctgaccga aaacggtacc
     2581 cacggttctg attccgtcga atctgccgct cgcgaaatcg cttatttctt cggcgaaggc
     2641 gaagtgtgcc cgcgcacccg ttaataattt cgtaaatgcc gcgtgcaaac gtggcatccg
     2701 tgcgccagaa tttgtacaat gcagcgcccc cggacgagca gccgctcacc ggggcgtttc
     2761 ttttttcaac cctccagggg ccataacgtg taataacgag gccggaatag catatgtctg
     2821 aacaattagt cacacctgaa aacgtcacca cgaaagatgg aaaaatcaac ctgctggatc
     2881 tcaaccgtca gcagatgcgg gagtttttta aagatttagg tgaaaaaacc ttccgcgccg
     2941 atcaggtgat gaagtggatg tatcactatt gctgcgacaa ctttgatgag atgaccgaca
     3001 tcaacaaagt gttgcgcggc aaactgaaag aggtggcgga aatccgcgca ccggaagtgg
     3061 ttgaagaaca gcgttcatcc gacggcacca ttaaatgggc gatcgccgtt ggcgatcagc
     3121 gcgtcgaaac ggtgtatatc ccggaagacg accgtgccac gctctgcgtc tcttcgcagg
     3181 tggggtgtgc gctggagtgt aaattctgtt ccaccgccca gcagggcttt aaccgcaacc
     3241 tgcgggtatc ggaaattatt ggccaggtgt ggcgtgcggc gaaaatcgtc ggcgcagcaa
     3301 aagtcaccgg tcagcgccca atcactaacg tcgtgatgat gggcatgggc gagccgctgc
     3361 tcaacctgaa caacgtcgtt ccggcgatgg aaatcatgct tgatgatttc ggttttggcc
     3421 tgtctaaacg tcgcgtgacg ctttccactt ccggtgtagt tccggcgctg gataaactgg
     3481 gcgatatgat cgacgttgca ctggcaattt ccctgcacgc gccgaacgac gaaattcgtg
     3541 acgaaattgt gccgatcaac aaaaagtaca atatcgaaac gttccttgct gcggtgcgcc
     3601 gttatctgga gaaatccaac gccaatcagg gccgagtcac tattgaatac gtgatgcttg
     3661 accacgttaa cgacggcact gaacacgcgc accaactggc ggaactgctg aaagatacgc
     3721 cgtgtaagat caacctgatc ccgtggaacc cgttcccggg cgcgccgtat ggacgcagtt
     3781 cgaacagccg tatcgaccgc ttctcaaaag tgctgatgag ctacggtttc accactattg
     3841 tgcgtaaaac gcgtggtgat gatatcgatg ctgcctgtgg tcagttggcg ggcgatgtta
     3901 tcgaccgtac gaaacgtact ctgcgtaaac ggatgcaggg tgaagccatc gatattaaag
     3961 cggtctgata gccattacgc cacggttact ttatgtaccg tggcgtaatc ttttatgaat
     4021 gaagtattaa gttgcaattg tctggtcaat attaacggtg cgtttttgct gactttaagg
     4081 cagtatgtaa cgatgcaaca gtaagttagc ctcagtgaat gtgggctttg tcacgagcac
     4141 acagacggtc ttatactgta tgataacggt taacttaacg gatgtttcgc ggtgtgggtg
     4201 agcatgatgg ttcaccggca tctcaattct catttaaacg tacctgcagc gaatgaatac
     4261 tgaagccacg cacgaccaaa atgaagcact tactaccggc gctcgcctgc gtaatgctcg
     4321 cgaacaacta ggacttagtc agcaggccgt tgccgagcga ctttgcctga aggtttccac
     4381 ggtacgcgac attgaagaag ataaggcacc cgccgatctt gcttcaacat tcctgcgcgg
     4441 atatatccgc tcttatgcgc gtctggtaca tattccagaa gaagaactgc tgccagggct
     4501 ggaaaagcag gctccacttc gggctgcaaa agttgcgcca atgcagagtt tttccctcgg
     4561 taaacgccgc aaaaaacgcg acggctggct gatgaccttc acctggctgg tgttgtttgt
     4621 ggttatcggc ctgagcggtg cctggtggtg gcaagaccac aaagctcagc aggaagagat
     4681 caccactatg gccgatcaat cttcggcgga actgagcagt aatagcgagc aggggcagag
     4741 tgttccgtta aatacgtcga caactacaga cccggctaca acgtccacgc cgccagcgtc
     4801 tgtggatact accgcaacca acacgcaaac acctgccgta actgcgccag caccagctgt
     4861 tgatccgcaa cagaatgcgg ttgtttcgcc ttcgcaggca aatgttgata ccgccgcgac
     4921 cccggtacca acggcagcaa caacgccaga tggtgctgcg ccgttgccaa ccgatcaggc
     4981 tggcgtgacc acgccggcgg ctgatccgaa tgcgctggtg atgaacttta ctgccgattg
     5041 ctggctggag gtcactgatg ctaccggtaa aaaattgttt agcgggatgc agcgtaaaga
     5101 cggtaatttg aatttaaccg gccaggcacc gtataaactg aaaattggtg cgccagccgc
     5161 agtacagatc cagtatcaag ggaaacctgt cgatctgagt cgttttatca gaactaacca
     5221 ggttgcgcgt ctgaccctca atgccgaaca atcaccggcg cagtaacaga cgggtaacgc
     5281 gggagatttt tcatgcataa ccaggctcca attcaacgta gaaaatcaac acgtatttac
     5341 gttgggaatg tgccgattgg cgatggtgct cccatcgccg tacagtccat gaccaatacg
     5401 cgtacgacag atgtcgaagc aacggtcaat caaatcaagg cgctggaacg cgttggcgct
     5461 gatatcgtcc gtgtctccgt accgacgatg gacgcggcag aagcgttcaa actcatcaaa
     5521 cagcgggtta acgtgccgct ggtggctgac atccacttcg actatcgcat tgcgctgaaa
     5581 gtagcggaat acggcgtcga ttgtctgcgt attaaccctg gtaatatcgg taatgaagag
     5641 cgtattcgca tggtggttga ctgtgcgcgc gataaaaaca ttccgatccg tattggcgtt
     5701 aacgccggat cgctggaaaa agatctgcaa gaaaagtatg gcgaaccgac gccgcaggcg
     5761 ttgctggaat ccgccatgcg tcatgttgat catctcgatc gcctgaactt cgatcagttc
     5821 aaagtcagcg tgaaagcgtc tgacgtcttc ctcgctgttg agtcttatcg tttgctggca
     5881 aaacagatcg atcagccgct gcatctgggg atcaccgaag ccggtggtgc acgcagcggg
     5941 gcagtaaaat ccgccattgg tttaggtctg ctgctgtcgg aaggcatcgg cgacacgctg
     6001 cgcgtatcgc tggcggccga tccggtcgaa gagatcaaag tcggtttcga tattttgaaa
     6061 tcgctgcgta tccgttcgcg agggattaac ttcatcgcct gcccgacctg ttcgcgtcag
     6121 gaatttgatg ttatcggtac ggttaacgcg ctggagcaac gcctggaaga tatcatcact
     6181 ccgatggacg tttcgattat cggctgcgtg gtgaatggcc caggtgaggc gctggtttct
     6241 acactcggcg tcaccggcgg caacaagaaa agcggcctct atgaagatgg cgtgcgcaaa
     6301 gaccgtctgg acaacaacga tatgatcgac cagctggaag cacgcattcg tgcgaaagcc
     6361 agtcagctgg acgaagcgcg tcgaattgac gttcagcagg ttgaaaaata ataacgtgat
     6421 gggaagcgcc tcgcttcccg tgtatgattg aacccgcatg gctcccgaaa cattgaggga
     6481 agcgttgagg gttcattttt atattcagaa agagaataaa cgtggcaaaa aacattcaag
     6541 ccattcgcgg catgaacgat tacctgcctg gcgaaacggc catctggcag cgcattgaag
     6601 gcacactgaa aaacgtgctc ggcagctacg gttacagtga aatccgcttg ccgattgtag
     6661 agcagacccc gctattcaaa cgtgcgattg gtgaagtcac cgacgtggtt gaaaaagaga
     6721 tgtacacctt tgaggatcgc aatggcgaca gcctgactct gcgccctgaa gggacggcgg
     6781 gctgtgtacg cgccggcatc gagcatggtc ttctgtacaa tcaggaacag cgtctgtggt
     6841 atatcgggcc gatgttccgt cacgagcgtc cgcagaaagg gcgttatcgt cagttccatc
     6901 agttgggctg cgaagttttc ggtctgcaag gtccggatat cgacgctgaa ctgattatgc
     6961 tcactgcccg ctggtggcgt gcgctgggta tcttcgaaca cgtaactctt gagctgaatt
     7021 ctatcggttc gctggaagca cgcgccaatt accgcgatgc gctggtggca ttccttgagc
     7081 agcataaaga aaagctggac gaagactgca aacgccgcat gtacactaac ccgctgcgcg
     7141 tgctggattc aaaaaatccg gaagtgcagg cgcttctcaa cgacgctccg gcattaggtg
     7201 attatctgga cgaggaatct cgtgagcatt ttgccggtct gtgcaaactg ctggagagcg
     7261 cggggatcgc ttacaccgta aaccagcgtc tggtgcgtgg tctggattac tataaccgta
     7321 ccgttttcga gtgggtgact aacagtctcg gctcccaggg caccgtgtgt gcaggcggtc
     7381 gttatgacgg tcttgtggaa caactgggcg gtcgtgcaac accggctgtc ggttttgcga
     7441 tgggcctcga acgtcttgta ttgttagtac aggccgttaa tccggaattt aaagccgatc
     7501 ctgttgtcga tatatacctg gtggcttcag gtgctgatac acaatctgcg gctatggcat
     7561 tagctgagcg tctgcgtgat gaattactgg gcgtgaaatt gatgaccaac cacggcggcg
     7621 gcaactttaa gaaacagttt gcccgtgctg ataaatgggg tgcccgcgtt gctgtggtgc
     7681 tgggtgagtc tgaagtggct aacggcacag cagtagtgaa ggatttgcgc tctggtgagc
     7741 aaacggcagt tgcgcaggat agcgtagccg cgcatttgcg cacgttactg ggttaaggaa
     7801 ggagaaggac agcgtggaaa tttacgagaa cgaaaacgac caggtagaag cggttaaacg
     7861 cttttttgct gaaaatggca aagcactggc tgttggggtg attttgggcg ttggcgcact
     7921 gattggctgg cgctactgga acagccatca ggttgattct gcacgctccg cttctcttgc
     7981 ctatcaaaat gcggttaccg cagtgagcga aggcaaaccg gatagcatcc cggcggcgga
     8041 aaaatttgct gctgaaaata aaaatactta tggtgcgctg gcttctttgg aacttgcgca
     8101 gcaatttgtt gacaaaaatg aactagagaa agctgccgcc cagttacaac aggggctggc
     8161 agacacgagc gatgaaaatc tcaaagccgt gataaatctg cgtcttgctc gcgttcaggt
     8221 acagctcaag caggctgatg ccgcgctgaa aacccttgat accatcaaag gtgaagggtg
     8281 ggctgccatt gttgccgacc tgcgtggtga agcattgctg agcaaaggtg ataagcaagg
     8341 tgcgcgtagt gcatgggaag caggcgtgaa aagcgatgtt actccggcac tgagcgaaat
     8401 gatgcagatg aaaattaata atttgtccat ctgagaggga cccgatgcaa ttgcgtaaat
     8461 tactgctgcc aggactgctt tccgttaccc ttttaagcgg ctgttcgctg tttaacagcg
     8521 aagaagatgt ggtaaagatg tccccattgc caaccgttga aaaccagttt acgccgacca
     8581 cggcgtggag cacttccgtt ggtagcggca ttggcaactt ctattccaat cttcatccgg
     8641 cactggcgga caacgttgtc tatgcagcgg accgcgctgg tttggtaaaa gcgctgaatg
     8701 cggatgatgg caaagaaatc tggtctgtca gcctggccga gaaagatggc tggttctcta
     8761 aagagcctgc attactttct ggcggtgtga ccgtgtctgg tgggcatgtc tacattggca
     8821 gcgaaaaggc gcaggtttac gcgctgaata ccagcgatgg tactgtggca tggcaaacta
     8881 cagtcgcggg tgaagcactt tcgcgcccgg tggtcagcga cggtctggtg ttaatccaca
     8941 ccagtaacgg tcagttacaa gcgctgaacg aagctgacgg cgctgtcaaa tggacagtta
     9001 acctcgatat gccttcgctc tctttgcgtg gcgagtctgc gccggcaacg gcttttggtg
     9061 cggccgtcgt gggtggcgat aatggtcgcg tcagcgcagt gctgatggaa cagggccaga
     9121 tgatttggca gcagcgtatt tcccaggcga ccggttctac cgaaattgac cgtctgagcg
     9181 atgttgacac gactcccgtc gttgttaacg gcgttgtttt cgcgctggcc tataatggta
     9241 acctgacggc gcttgatctg cgcagtggtc agattatgtg gaaacgcgaa ctgggttcgg
     9301 tgaatgattt catcgtcgat ggcaatcgca tctatctggt cgatcaaaat gaccgggtga
     9361 tggcgttgac cattgatggc ggcgttacgc tgtgggcaca aagcgatctg ctgcatcgcc
     9421 tgctgacttc tccggtgctg tataatggca acctggtggt cggtgacagt gaaggttatc
     9481 tgcactggat taacgtcgaa gatggtcgtt tcgttgccca gcaaaaagtt gatagttccg
     9541 gtttccagac tgaaccggtt gccgctgacg gcaaactgct gatccaggca aaagacggaa
     9601 ccgtgtactc tattacacgt taatcgtctc tgtcgttcac tttgaaaacg gctcctggac
     9661 aggggccgtt ttcctgtttt taacaacgac gcgaatatag cgtgcgttgt ctgatgattt
     9721 ataaaaatga ggctttaaac atggtacctg tggtcgcgct tgtcgggcgc cctaacgtag
     9781 gaaaatccac gttatttaac cgtctaactc gcacccgaga tgcgctggtt gcggatttcc
     9841 cgggtctgac tcgtgaccgt aagtacggtc gtgcggaaat tgaaggccgt gagtttatct
     9901 gtattgatac cggcgggatt gatggcacag aagacggtgt agaaacccgc atggcggaac
     9961 agtcgctgct ggcgattgaa gaagcggacg tcgtactgtt tatggtggat gcgcgcgcgg
    10021 gcctgatgcc ggcagatgaa gcgattgcca aacatctgcg ctcccgtgaa aaaccgacct
    10081 tcctggtggc aaacaaaact gacggtctgg atcccgatca ggcagtggtt gatttctact
    10141 cgcttggttt aggtgaaatc tacccgatcg ccgcgtctca cggtcgtggc gtattaagtc
    10201 tgctggagca tgtgctgctg ccgtggatgg aagatctcgc accgcaagag gaagtcgacg
    10261 aagacgctga atactgggcg caatttgaag cggaagagaa cggcgaagaa gaagaggaag
    10321 acgacttcga cccgcaaagt ctgccgatca aactggcgat tgtgggtcgt ccgaacgtag
    10381 gtaagtctac actcactaac cgtattcttg gagaagagcg cgttgttgtt tacgacatgc
    10441 ctggcacgac gcgtgacagc atctacatcc caatggaacg cgatggacgt gagtatgtgc
    10501 tcattgacac cgctggcgta cgtaaacgcg gcaaaatcac cgatgctgta gagaaattct
    10561 ccgtaatcaa aacgttgcag gccattgaag acgccaacgt ggtgatgtta gtgattgatg
    10621 cgcgcgaagg tatttccgat caggatctct cgctgctggg ctttattctc aatagtgggc
    10681 gctcacttgt cattgtggtg aataagtggg atggcctgag ccaggaagtg aaagagcagg
    10741 tgaaagaaac gctggacttc cgtctgggct ttatcgattt tgctcgtgtg cactttatct
    10801 ctgccttgca cggcagtggt gttggtaact tgtttgaatc agtacgtgaa gcgtatgaca
    10861 gctccacccg tcgtgtgggg acctctatgc tgacgcgcat catgacgatg gctgttgaag
    10921 atcaccaacc gccgctggta cgcggtcgtc gtgtgaagct gaaatatgcc cacgctggtg
    10981 gttataatcc gccgattgtg gtgattcacg gtaatcaggt gaaagacctg cctgattcct
    11041 acaagcgcta cttgatgaac tacttccgca aatcgctgga cgtaatggga tcgccgattc
    11101 gtattcagtt caaagaaggg gaaaacccgt atgcgaacaa gcgtaacacc ctgacgccaa
    11161 cccagatgcg taaacgtaag cgtctgatga agcacatcaa gaaaaataaa taattatccg
    11221 taagtatttt atctgccatt ccctctatga atgtagaggg aatggctacc ggaggaattc
    11281 atatggaact gcattgtcca aaatgtcagc atgtgcttga tcaggataac ggccatgcgc
    11341 gttgcccatc ctgcggggaa tttattgaaa tgaaagctct ttgtccagac tgccatcaac
    11401 cgttgcaggt gttaaaagcc tgcggtgcag tagattactt ctgtcagcac gggcatggct
    11461 tgatatcgaa aaaacgcgtt gaattcgtgc tggcttaatg aaccttttta cgcgattttt
    11521 ttaccggctg gatgtttttt acttcacttt ctatccagcc gtcttccaga cgtgtggtta
    11581 gcatttcacc cgctttaact tgcttcactt ttttcagtac attgccgtca gtagcagtag
    11641 taacgctgta tccacgcgcc agcgttgaca gtgggcttac ggcttcgagg tgcgttactg
    11701 cattaccgaa acgttcacgc gtggcgctaa gctgtgcgcg caggatttct gctaaacgat
    11761 attccagttg ctgaatgcgc gtttgcgtgc gatgaatctt cggctgtgga ttttgctgat
    11821 tcagccgctg tgttaatcgc tgctgctgtt gcccggcacg cttaagctga ttttccagcg
    11881 caaagctcat ccgtttttgc aggcgttcaa gcatggtttg ctggcgtgcc agccggagct
    11941 gcggatgctg ctgctgcaag cgatgatgaa tctgcgtaaa gcgacgcgta cggttggcga
    12001 gataataatc catcgccatc tccagccgtt gacgggccga ttgcacctgg cgcagtaact
    12061 cttgctgatt acggctcact acttcagcgg cggcagacgg cgttggcgca cgcagatcgg
    12121 caacaaaatc ggcaatggtc acatccgtct catgcccgac ggcgctgacg accggaatgc
    12181 ggctggcaaa aatcgcccgc gctacgcgtt cgtcgttaaa actccataaa tcttccagcg
    12241 aaccgccgcc gcgcccaacg atcaacacgt cgcactcatt gcgctgattc gccagttcaa
    12301 tggcgcgaac aatttgcccc ggcgcgtcat cgccctgaac ggaggtgggg tagatgatca
    12361 ccggtagaga aggatcgcga cgttttaaca catgcaaaat atcatgtagc gcagcaccgg
    12421 tttttgaggt gatcacacca acgcaatgcg caggggaggg aagtggtttt ttgtattgct
    12481 gatcgaacaa accttcagcc tgcaacttcg ctttgagctg ttcgtacttc tgttgcagca
    12541 gcccttcacc ggccggctgc atactctcaa cgattatctg gtagtcgccg cgcggctcgt
    12601 agagcgtaat attggcgcga actaaaactt gttgcccatg ctgcggacgg aaggtgaccc
    12661 ggcggttgct gttgcggaac atcgcgcagc gtacctgggc ggtgtcgtct ttgagtgtaa
    12721 agaaccagtg accggaagcc ggttgtgtga aattagagat ttcgccgctg atccaaacct
    12781 gtcccatctc atgctcaagc agcagacgaa ccgtttgatt caggcgacta acggtaaaaa
    12841 ttgcagggga ttgagaaggt aacatgtgag cgagatcaaa ttctaaatca gcaggttatt
    12901 cagtcgatag taacccgccc ttcggggata gcaagcattt tttgcaaaaa ggggtagatg
    12961 caatcggtta cgctctgtat aatgccgcgg caatatttat taaccactct ggtcgagata
    13021 ttgcccatgc tacgtatcgc taaagaagct ctgacgtttg acgacgttct cctcgttcct
    13081 gctcattcta ccgttctgcc gaatactgct gacctcagca cccagctgac gaaaactatt
    13141 cgtctgaata tccctatgct ttccgcagca atggataccg taacggaagc gcgcctggct
    13201 attgctctgg ctcaggaagg cggtatcggc tttatccaca aaaacatgtc cattgaacgc
    13261 caggcagaag aagttcgccg tgtgaaaaaa cacgaatctg gtgtggtgac tgatccgcag
    13321 actgttctgc caaccacgac gctgcgcgaa gtgaaagaac tgaccgagcg taacggtttt
    13381 gcgggctatc cggtcgttac cgaagaaaac gaactggtgg gtattatcac cggtcgtgac
    13441 gtgcgttttg ttaccgacct gaaccagccg gttagcgttt acatgacgcc gaaagagcgt
    13501 ctggtcaccg tgcgtgaagg tgaagcccgt gaagtggtgc tggcaaaaat gcacgaaaaa
    13561 cgcgttgaaa aagcgctggt ggttgatgac gaattccacc tgatcggcat gatcaccgtg
    13621 aaagacttcc agaaagcgga acgtaaaccg aacgcctgta aagacgagca aggccgtctg
    13681 cgtgttggtg ctgcagttgg cgcaggtgcg ggtaacgaag agcgtgttga cgcgctggtt
    13741 gccgctggcg ttgacgttct gctgatcgac tcctcccacg gtcactcaga aggcgttttg
    13801 cagcgtatcc gtgaaacccg tgctaaatat ccggatctgc aaatcatcgg cggcaacgtg
    13861 gcaacagctg caggtgcacg cgctttggca gaagctggtt gcagcgcggt taaagtcggt
    13921 atcggccctg gttctatctg tacgactcgt atcgttactg gcgtaggtgt tccgcagatc
    13981 actgccgttg ctgacgcagt tgaagccctg gaaggcaccg gtattccggt tatcgcagat
    14041 ggtggtattc gcttctccgg cgacatcgcc aaagctatcg ccgctggcgc aagcgcggtg
    14101 atggtaggtt ccatgctggc gggtactgaa gaatctccgg gtgacatcga actctaccag
    14161 ggccgttctt ataaatctta ccgtggtatg ggttccctgg gcgcgatgtc caaaggttcc
    14221 tctgaccgtt atttccagag cgataacgct gccgacaaac tggtgccgga aggtatcgaa
    14281 ggtcgcgtag cttataaagg tcgcctgaaa gagatcattc accagcagat gggcggcctg
    14341 cgctcctgta tgggtctgac cggctgtggt actatcgacg aactgcgtac taaagcggag
    14401 tttgtacgta tcagcggtgc gggcattcag gaaagccacg ttcacgacgt gaccattact
    14461 aaagagtccc cgaactaccg tctgggctcc tgattctctt cgcccgactt catgtcgggc
    14521 gatttatatt atctgtttca cttgcctcgg aataagcgtc aatgacggaa aacattcata
    14581 agcatcgcat cctcattctg gacttcggtt ctcagtacac tcaactggtt gcgcgccgcg
    14641 tgcgtgagct gggtgtttac tgcgaactgt gggcgtggga tgtgacagaa gcacaaattc
    14701 gtgacttcaa tccaagcggc attattcttt ccggcggccc ggaaagtact actgaagaaa
    14761 acagtccgcg tgcgccgcag tatgtctttg aagcaggcgt accgatattc ggcgtttgct
    14821 atggcatgca gaccatggca atgcagttgg gcggtcacgt tgaagcctct aacgaacgtg
    14881 aatttggcta cgcgcaggta gaagtcgtaa acgacagcgc actggttcgc ggtatcgaag
    14941 atgcgctgac cgcagacggt aaaccgctgc tcgatgtctg gatgagccac ggcgataaag
    15001 ttaccgctat cccgtccgac ttcgtcaccg tagccagcac cgaaagctgc ccgtttgcca
    15061 ttatggctaa cgaagaaaaa cgcttctatg gcgtacagtt ccacccggaa gtgactcaca
    15121 cccgccaggg tatgcgcatg ctggagcgtt ttgtgcgtga tatctgccag tgtgaagccc
    15181 tgtggacgcc agcgaaaatt atcgacgatg ctgtagctcg catccgcgag caggtaggcg
    15241 acgataaagt catcctcggc ctctctggtg gtgtggattc ctccgtaacc gcaatgctgc
    15301 tgcaccgtgc tatcggtaaa aacctgactt gcgtattcgt cgacaacggc ctgctgcgtc
    15361 tcaacgaagc agagcaggtt ctggatatgt ttggcgatca ctttggtctt aacattgttc
    15421 acgtaccggc agaagatcgc ttcctgtcag cgctggctgg cgaaaacgat ccggaagcaa
    15481 aacgtaaaat catcgggcgc gttttcgttg aagtattcga tgaagaagcg cagaaactgg
    15541 aagacgtgaa gtggctggcg cagggcacca tctaccctga cgttatcgaa tctgcggctt
    15601 ctgcaaccgg taaagcacac gtcatcaaat ctcaccacaa cgtgggcggc ctgccgaaag
    15661 agatgaagat gggcctggtt gaaccgctga aagagctgtt caaagacgaa gtgcgtaaga
    15721 ttggtctgga gctgggcctg ccgtacgaca tgctgtaccg tcatccgttc ccgggaccag
    15781 gccttggcgt tcgtgttctg ggtgaagtga agaaagagta ctgtgacctg ctgcgccgtg
    15841 ctgacgccat cttcattgaa gaactgcgta aaacggacct gtacgacaaa gtcagccagg
    15901 cgttcaccgt cttcctgcca gtacgttccg ttggcgtaat gggcgatggt cgtaagtatg
    15961 actgggttgt ctctctgcgt gctgtcgaaa ccatcgactt tatgaccgca cactgggcgc
    16021 atctgccgta cgatttcctc ggtcgcgttt ccaaccgcat tatcaatgaa gtgaacggta
    16081 tttcccgcgt ggtgtatgac atcagcggca agccgccagc taccattgag tgggaatgat
    16141 tttgatccct gcactatgaa tgaacaaaac cctccgttac tacagagggt tttttatctt
    16201 caagaatcat aggcttgaag ttactaacat cgattaatta aaccagctgt ccgatttgtt
    16261 ctcttctgct ttgcccacgc ttttcatcag atcgcgaccg ccttcagtca tatttctgtt
    16321 tgcgtcagct tcagattgca ccacatcggt ttgcgcagct ttgtgcttca gttcctgatc
    16381 gataaattcg ttttcgcgct taacgcgggc ttcttctttc gccagcgcca gtttttgttt
    16441 ctgaatctct aagctgcgta gctcatcttc ataactttga tcgcgttttt tgtccgcagt
    16501 ggcttcggcg tccagtttat cctgacgagc tttcttattt gccgctgccg ttgccgctct
    16561 tttgttcgcg gcggcctggg catttgcgcg acgttgcttc tcttgctgga tttccctgtt
    16621 gcgctccgcg acccattcgt catgctgcct ttgctcttca tttttacctt gctgttccgc
    16681 ttctgcgaca gcagagagtt gatcctgcaa tgatgaggct atagccggat agcttaagga
    16741 ggctaagatg gcgcaaagaa aaactttctt catgactcct cctgattatt agctcttttc
    16801 aggacatttg gtatttggct gaatacgcgt ttcgttatac gttgtggtaa taacaacggc
    16861 taaacctgtc gtaaactggc actctttacc ctcctgggtg gaggtataca ctttggtgcc
    16921 ttccttatat gttaaagaaa caccttccac taaggtttta tcattcacca tagaacccgc
    16981 tgccgcgcct acagctccgc cgccaactgc ccctgccgtc gttccggaat tgctgccaga
    17041 accgacgttg tggccgataa caccgccagc gactgcgcca ataagcgcgc cgaaggcttg
    17101 tgcgttccgt ttattttggg agttgtctac ggcaactttt gcgggaagaa tggaaataat
    17161 attaacggtt ttagtttctt gtttggtatt cagttgatcg gtttgataaa catcggcggc
    17221 atgatcgtca gcatttgact ggcatcctgc cagagtgaat gacgctaaca ttgccacagg
    17281 cagaagacat tttttaaatt tcatcactat tccttgttat acacattacg aaaaataaga
    17341 catttaagat catcaaaaga ggaaagtaaa tgtattgcgt ataaagggat agattaaatt
    17401 tattaaaatt cacgcaggga ataatttata aaaatatatc attacaacat atatattaat
    17461 tttatattta tttcgcagcc aaacaggata aacatcagtc aggacaaaga cgtaaaattt
    17521 aagatgaaag aatatctgcc gctttgataa tcccaaagag caaaagcatc cgacagcgaa
    17581 gtgccgttat gttctgtgaa agtggagaag gtaaccgcta acgttgtacc ggtgattcga
    17641 cggagagtgt ggattgctga gcttcttttt tgctctgatg gtgcagccag gcaccgacgg
    17701 acgagtagat aaaacggcca aagaagaaga taaagctgat gagcagtacg atacgggtca
    17761 tgcgactgtt aaatcggtgt cgttttcgca tactagttgc ctgactcaca aaaggttcct
    17821 tgaagtatgt cccacgctgt ggacggtact tacattaagg cacaacagga caaaatggtc
    17881 aattcttcgt tatgtaaaaa agcgtcagtg gatacatatt ttaatgttat ggaagttaat
    17941 ttaattattt acaatgatga tgtaataatg atgaattaac atgataaacg taataattca
    18001 ttaatcaggg ttattttgtt tgatatatat caattggaac tttcatatga cacttagaat
    18061 caatgctctc tatagtgatg aataatcatc attcgaagtc aggtgggatg cctgtctgaa
    18121 tacacctcct tcaggatgtg ggggattctg ctgagcatct atgaaactga atgca
//
LOCUS       DAJTYG010000074        18158 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTYG010000074 DAJTYG010000000
VERSION     DAJTYG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.074"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..719)
                     /gene="aroD"
                     /locus_tag="OO568_002762"
     CDS             complement(<1..719)
                     /gene="aroD"
                     /locus_tag="OO568_002762"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6642055.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISV"
     gene            complement(750..1616)
                     /gene="ydiB"
                     /locus_tag="OO568_002763"
     CDS             complement(750..1616)
                     /gene="ydiB"
                     /locus_tag="OO568_002763"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6642056.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1628..2899)
                     /locus_tag="OO568_002764"
     CDS             complement(1628..2899)
                     /locus_tag="OO568_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6642057.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3082..4286)
                     /locus_tag="OO568_002765"
                     /pseudo
     CDS             complement(3082..4286)
                     /locus_tag="OO568_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4386..4742)
                     /locus_tag="OO568_002766"
                     /pseudo
     CDS             complement(4386..4742)
                     /locus_tag="OO568_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(4878..4985)
                     /gene="rprA"
                     /locus_tag="OO568_002767"
     ncRNA           complement(4878..4985)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO568_002767"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5171..6283)
                     /gene="ydiK"
                     /locus_tag="OO568_002768"
     CDS             complement(5171..6283)
                     /gene="ydiK"
                     /locus_tag="OO568_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6642058.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6672..9728
                     /locus_tag="OO568_002769"
     CDS             6672..9728
                     /locus_tag="OO568_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6642059.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9725..10135
                     /gene="menI"
                     /locus_tag="OO568_002770"
     CDS             9725..10135
                     /gene="menI"
                     /locus_tag="OO568_002770"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6642060.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10235..10423
                     /locus_tag="OO568_002771"
     CDS             10235..10423
                     /locus_tag="OO568_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6642061.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            10971..11339
                     /gene="sufA"
                     /locus_tag="OO568_002772"
     CDS             10971..11339
                     /gene="sufA"
                     /locus_tag="OO568_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6642062.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEIDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11348..12835
                     /gene="sufB"
                     /locus_tag="OO568_002773"
     CDS             11348..12835
                     /gene="sufB"
                     /locus_tag="OO568_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6642063.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12845..13591
                     /gene="sufC"
                     /locus_tag="OO568_002774"
     CDS             12845..13591
                     /gene="sufC"
                     /locus_tag="OO568_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6642064.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETHDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13566..14837
                     /gene="sufD"
                     /locus_tag="OO568_002775"
     CDS             13566..14837
                     /gene="sufD"
                     /locus_tag="OO568_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6642065.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGFKQQVLARIGQRLPGGAR"
     gene            14834..16054
                     /gene="sufS"
                     /locus_tag="OO568_002776"
     CDS             14834..16054
                     /gene="sufS"
                     /locus_tag="OO568_002776"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6642066.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALGCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16067..16483
                     /gene="sufE"
                     /locus_tag="OO568_002777"
     CDS             16067..16483
                     /gene="sufE"
                     /locus_tag="OO568_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6642067.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16632..17636
                     /gene="ldtE"
                     /locus_tag="OO568_002778"
     CDS             16632..17636
                     /gene="ldtE"
                     /locus_tag="OO568_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6642068.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17700..17936)
                     /gene="lpp"
                     /locus_tag="OO568_002779"
     CDS             complement(17700..17936)
                     /gene="lpp"
                     /locus_tag="OO568_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6642069.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4773 a         4388 c         4669 g         4328 t
ORIGIN      
        1 tttaccgaga tttgccctgg cgcagaggct ttttttaccg caccaaaagt tgccgccgag
       61 ccaaatactt caccagccag acgagaaatt acgccagttt ttgccatcga catcgtgatg
      121 attggacgat cggcatactg ctcttgcatc tccagggtcg cggcaagcaa cgtcagcaca
      181 tcgctggtac tttgcggcat cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt
      241 ttgcgcagac gggcaatgat ttcttcggct tccggcgttt tatggaagtc atggttggac
      301 atgacaactt tcacatcatg cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg
      361 ccggtaaata actccagatc gatcatatca accaggccgc tgtcgatggc tgcacgattg
      421 agagcaatat aagcctcggt ggaaatcgcc tgctcgccgc cttctttggc actgcggaag
      481 gtaaacagca gcggtttttc tggcatggtt tcacggagaa tttttgccgc cgccatgaca
      541 gactccacat tggagaggtc ggcaaagtgg tccacacgcc attccagaat atcaaagtcc
      601 gcttcacgat aggcgagagc ttcggatttc acgcgggcga tatctttcgc catcagcgag
      661 acgatgattt taggtgcgcc cgcaccaatg acgagatctt ttacagttac ggttttcatt
      721 ttttaccctt tctgcacgcg gtcagcctgt caggcaccga accccatgac ctgtttaaca
      781 tattccagag ggaaatcttt gccagtccat aacgtgaact gttcagcccc ttgccacaac
      841 aacatgccgt atccatcaat cgttttgcaa ccagcttgtt gtgcctgctg caataacttc
      901 gtcatatgcg ggttatacac gcattcagtg accagcagtc ccggatgtaa cagactgata
      961 tcattaacca atgattcatt ctcaaggggt ttcataccta cttttgtgcc attggttaaa
     1021 atgtcggcgg aagccagggc ttcagcaaag gcttgctgat cggcgagatc ggtaaccgtg
     1081 acgacacaat cggtgttttc gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac
     1141 tcatcccgac ggttaaagag tttaatttct tttaaacctt caattgcccc ctgcgcgcca
     1201 atcgccgttg aggcacctcc ggcccctaac agcaccatcg ttttgccttt gatatcaaaa
     1261 ccgctctctt taatggcgcg aatatggccc gtgccgtcgg tgttatagcc acgcagatag
     1321 ccatcatcat taacgatggt gttgatggca cccaccagtt tggctgctgg cgttaattca
     1381 tcaacatatt cacacgccag ttgtttgttc ggcatcgaca caccagttcc gcgcattttg
     1441 agggctttta atccttcaat tgctccggga aagctatcgt tatccacttc gaaggccata
     1501 taggtaaatg gcaatcccgc tttttctaag gctttattct gcatttcagg cgataaactg
     1561 tggcggatag gataagccat caatccaatc aattcgtatt ttgcggtaac atccataatt
     1621 aactccttta acctctatgc ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa
     1681 accgcacatc attctcagga ataataaaaa cgcggtaata gcggctaaaa acaataattg
     1741 cggtaatcag ggccagcagc gcgaaagtaa aatcgagaac aatgatatat tgcaggccga
     1801 tgttcgacag ataaccggta atcagcggaa taacaaagtt agccagtcca cccatcatca
     1861 tataaatact ggtgactttg gctttgcttt tggggaaaaa ctctgacatg accgaaacac
     1921 cgagctgtaa aatgccgcca gctgctgaga aaccgataac aaaggcgccg gcattacaca
     1981 ccagtggaga agggtacagg tagataatgg cagctgttat tgttgccagc gcagagttaa
     2041 atacattagc ccagatgggc cggaccattt ttttcagtag tgcggcaaaa ataaagacac
     2101 agaccaacga gcccatactg taataagaga tggtttttaa tgcctcggct tctgacatac
     2161 cagcaaaagc catcgcatat ttgggcatcc acaccacaat cacataaaag gtcgagaatg
     2221 cggctacacc gaacagtacc gatgaaacac cttccagcca gactaacggt ttgctgttca
     2281 tttgcggtaa ttcattagct acgctggcgt ccaccaactg gctggggaat ttgcttttca
     2341 acagcatcag cgtgatcagt acaaacagaa tacccggaat aatcagccca tagccgtacc
     2401 agatattatt gagcaacata tagctcacca gcattgggta gaacatttgc ccaaatgaca
     2461 ccatcgcttt aaccagtatg accgccgaac cagaggcttt cggaaagcat tccatgagcg
     2521 cggggtagcc acccgtatcc agcgctgagt tagcgatacc tacgcacact gccagaccgt
     2581 aggcgagagt taaattcggg caagcgggaa taccaaagaa gaatagcaga tacattatta
     2641 ctgccattaa tatcaccgcc cgacgaccaa acttatcgga gatcacaccg aagaataaaa
     2701 tactgatcaa tcgccccaaa ccgataccgg aaattaaata ggcaatgccc gcgttgtcag
     2761 tggaaaactt ttctgccaga gatgtcatat tttgggcaag cgtaataaca ctaatgccgt
     2821 gcaggaaata gctgaagtaa atacaaagaa cagccaggat aaatggcgtg ctgaaagata
     2881 atttcgttac cgtacgtatt gttctccgac ggtttacata attactatac tgaagatgat
     2941 tttccctggg tacatggcat aaagtttatg tttccaggaa tattgcatgt catagtttgt
     3001 ttttgatcgc gattttcctt agttagcgca gaaagagtta tttatccgta agatcttgca
     3061 tgacctgaaa agaatattgc attacccacc cggagcgaca ttttccttta gcgagtgatg
     3121 ccgcgttttt ttgcggctgc gtaagccgat aaacagtgcc agtaaaaaac cgatggcagt
     3181 atcgaaccac ataatatcgg caatacttct ttgggacaga tgagccgtaa tcaacggaat
     3241 agtaaaggtc gcaatactgc ccgcactgta atagatccct gtagctttac ctttagcgta
     3301 agggaaacgt tcagctatta acgtcaggcc aatttgcaca acacctccag cagaggtaaa
     3361 accaatgaca aaagcaaata ttatcaccac ataaaatgtg ggatgcaggc agacggtaaa
     3421 cagagcaata aatgagataa aggtgtacag catcagtaat gttgttgggc gaacggtatt
     3481 acgaatgagt ggagcggtaa taaatacaca aagcagcgaa cccacggtgt agatactgag
     3541 tagtttgatc gacatagtgt atgacatgcc tgcaacaaat tgtccgtact gtgccagcca
     3601 ctggctaacc agataaaacg ttgccattga gatatagcca tataaggtat aactggctaa
     3661 atcgataatt gaacagcgat gttccgtaga gctggtggtt ttctttatga caggtaagcg
     3721 acgacccgga tggggtggga acgtacaacg gtataaaaac agagcgttaa taaacataat
     3781 gcctgcagca atcataaagg accaaccgaa ccacagttca gcccacacta acaggctaat
     3841 gattagcggt aataaaaatt gtccgctgga aacaaatgct ttaattaaaa tattggctgt
     3901 tccaggtgag cgtggaaaag cttccatcaa actgggataa gtgcctgcat cgagaaaact
     3961 gtttgccatt cccgccagaa agccaaaaac ataagcgata atgatgttat tggtctgcag
     4021 gatgccaaaa aagaaggcca tatagcagca cattccgagc atgataaaag ggcggcgacc
     4081 aaagcgatcg gataataatc ctgcaaaaag caagacactt aatcgaccaa tgcccagcga
     4141 tgagataact atcgagacac ccgcggcatt agtctgacaa agtgtctcca gcgaggccat
     4201 attcaggctc atcaaaatga cgcccatacc atgcaccagg taataaaata caacccaagt
     4261 gcggtagggt aatagggatt tttcataggt tctacttagc aaaggtgctt taaaaagtgt
     4321 agcgatgttg tgtcagtatc cattcattat tgttgtaaca acgacaattc ctttctgtgg
     4381 aaaaattaac aaagccgctc cagatcgtgt gcatataatt cagcggctac cgattgatag
     4441 atcttccagt caataaaatt gccatcaggg tagacttgtt gaaacgcggt taagtcgggg
     4501 aaaaaacgca tggtgttgtt gccgatgcgg ttatttattt tctctatgat ggcatgaaga
     4561 tgtttttttc gttactgacg catagccaac agttgctcga ctacacaatc aggaatggcg
     4621 catttgccat tttcccactg acgccagctt tcactgtcac cggtctgggc aatccaggtc
     4681 gcgcattcat caatagtcat ggcaaaaata tggcggagtg cctggagttc ataagcgttc
     4741 atcgtgtaat ggctgcatga aataaacaat aaaatataaa taacattatc agcctgctga
     4801 cggcttgaag agagtcacag tatcttgtgc aacattattg gaaagagtga ggggcgaggt
     4861 agcgaagcgg aaaaatgtta aaaaaaagcc catcgtggga gatgggcaaa gactacacac
     4921 agcaattcgt tgtttcactc aggggatttc catgcttata aatcaatatg ttgatttata
     4981 accgtgagct aatagtaggc atacggactt tttgcgtcga tcagattcgt ctcattagtt
     5041 taaaaactgt aaagaatttg cgacaggtac taccagctga atggcggtaa gcaggaaaat
     5101 atggggaaga gcaagcaaat atcaattagc ggtgattaac aatcaggcgg cagagccgcc
     5161 tgatgagaaa ttacttattc ggtttctcga tttcgccaag ttcttcgaga atttcttccg
     5221 gttggtccgt cggcggcggg acttcttcca cccacgcggc aaacagacgc caggaaacgg
     5281 ctaacagaac cggaccaata aacagaccga tcatcccgaa agcaatcaaa ccaccaataa
     5341 cgccagagag gatcaggatc agcggtaaat cggcacccat gcgaattaac attgggcgga
     5401 tgacgttatc cagcgtgcca accacaccgc tccacactaa caataccgtt ccccaggtgg
     5461 tatcgccggt ccagtagagc cagataatcg ccggaatcag taccggcaac gggccaagct
     5521 ggacaaggca ggagaggatc attaacaccg ttagcagcgt tgcataaggt acgccggata
     5581 cggcgaggcc gataccgcca agcactgcct gtactaacgc cgtcaccacc acacccagcg
     5641 ccaccgcgcg gatagcctgt gccgccagca gcacggcggc atctccgcga acgcctgcca
     5701 gacgggttgc aaaatggcga atgccttgtg ccacctgttc accgcgccag tacagcaggg
     5761 cactgaagag aagcatcagc gcacaatgca ccataaagcg cccgatatgc gccgcctgcc
     5821 caacgaacca ggtggtggtg gtgccaatat aagggcggac tttcgccatg atcgccgtac
     5881 cccccatatc cagcaagttg tgccagcctg catacagctt cgcgccaatc accggaatgg
     5941 tattaagcca cgccagatcg ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg
     6001 ggccgctgcc gtcgacgata ctgttaacca gcaaagcaat agggatgata aacaccatca
     6061 ctaataacag cgtcatcacc agaacggcga gagagcggcg gccaaacatg atcttttgca
     6121 aacgtaacaa taccggccag gtggcgataa ccaccgtacc ggcccatgca aagccgagaa
     6181 taaagggttg aacaatccac agacatgcca caatcatgat ggctaaaaac agcaccgaaa
     6241 gcagaatttg tgcgacatcc ctgggctgac gaacatttac cataactact tttcaccttt
     6301 tttgtgcgtc aaatcgtcga cgcgacgtgg acacgcgaaa ctcacctgca taatgatgag
     6361 caatttcagc ggtttttaac aggccgattc tgtccttaat tctgatgggc gtgttagaaa
     6421 aaaatgtggt acaacaactc aggcaacacg caaacgattt actcgtcgta tttcaagttg
     6481 catgtgctgc ggctacgttc gttcacccca gtcacttact attgtaagct cctggagttc
     6541 actcgcttgc cgccttcctg caactcgaaa tccatagagt aaataaacat cacctttcag
     6601 tgcgctattg cagccgcctg aaagaatagt gtatcgacct acatcacaga ccgcaggaaa
     6661 gggtcaatat aatgattcca cagatttccc aggcacccgg cgtcgttcaa ctggtgctta
     6721 attttttgca agagctggag caacaaggtt ttaccggcga tgcggcgaca agttatgccg
     6781 atcgtctgac aatgtcgacc gacaacagta tttaccaact tctccccgat gcggtggtat
     6841 ttccgcgttc aaccgcagat gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt
     6901 catcgctgat ctttaccccc cgcggcggcg gcaccggcac taacggtcag gcgctcaacc
     6961 aggggattat tgttgatatg tcccgccata tgaaccgcat catcgaaatt aaccctgaag
     7021 agggctgggt gcgcgttgag gccggggtga taaaagacca actcaatcag tacctgaaac
     7081 cgttcggcta cttttttgcg ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga
     7141 tgatcaatac cgatgcatcc ggtcagggat cgctggtcta tggcaaaacg tcagatcacg
     7201 tacttggcgt acgcgcggtg ttgttggggg gcgatattct cgatacgcaa cctttacccg
     7261 tcgagctggc ggaaacgctg ggtaaatcca ataccacaat cgggcgaatt tataacacgg
     7321 tttatcaacg ttgccgtcag caacgccagt taatcatcga caacttcccc aaacttaacc
     7381 gctttcttac cggttacgat ctgcgtcatg tctttaacga tgagatgacc gagttcgacc
     7441 tgacgcgcat tctgacgggt tcagaaggga cgctggcctt tattaccgaa gcgcggctgg
     7501 atattacgcg cttgcctaaa gtgcgccgtc tggtgaatgt caaatatgac tcttttgact
     7561 ctgcgctgcg taacgcgccg tttatggttg aggcgcgggc gctttcggta gagacggtgg
     7621 actcaaaagt gctgaatctg gcgcgggaag atattgtctg gcattccgtc agcgagttga
     7681 ttaccgatgt gcctgacaaa gagatgctcg gactgaacat tgtggaattt gctggtgatg
     7741 atgaggcgct gattgacgag cgagttaatg cactctgtgt gcggcttgat gagctgattg
     7801 ctagccaaca ggcaggtgtg attggctggc aggtgtgccg cgagctggcg ggggttgaac
     7861 gtatctatgc gatgcgcaaa aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta
     7921 agccaattcc gtttgctgag gatacctgcg taccgccgga acatctggcc gattatattg
     7981 ctgaatttcg cgcgctgctc gacagccacg gcttaagcta cggtatgttc ggtcacgtcg
     8041 acgcaggtgt cttgcacgtc cgtccggcac tggatatgtg cgatccgcaa caagagattt
     8101 tgatgaagca aatctctgat gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt
     8161 ggggcgagca cggcaaaggt tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac
     8221 tttttgcaga actgcgcaaa gtgaaagcgg catttgaccc gcataaccga ctcaacccag
     8281 ggaagatttg cccgccagaa ggcctcgatg cgccgatgat gaaagtggac gcggtgaagc
     8341 gcggtacatt cgatcggcag atccccattg cggtacgcca gcagtggcgc ggtgcgatgg
     8401 agtgtaacgg caacggttta tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga
     8461 tgaagatcac ccagaaccgg attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat
     8521 ggctgcgctt gttggcggat cgcggcgttg acccactcaa actggaacaa gaactgcctg
     8581 aatccggcgt cagtttgcgg acgttaattg cccgcacgcg caatagctgg catgcgaata
     8641 aaggcgaata tgacttctca cacgaagtca aagaggcgat gtcgggctgt ctggcctgta
     8701 aagcttgttc gacccagtgc cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc
     8761 tgcagctcta tcacacccgt tatttacgcc cgcttcgcga ccacctcgtt gctacggtcg
     8821 agagctacgc accgctgatg gcacgcgcgc cgaaaacctt taacttcttc attaaccagc
     8881 cgctggtgcg caaactctcg gaaaaacata tcggcatggt tgatttgcca ctgctgtcgg
     8941 tcccctcgct acaacaacaa atggtggggc atcgctcggc aaacatgacg ctggaacagc
     9001 ttgaagctct caatgcagag cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta
     9061 ccagctatta cgatgcgcaa gtggtggcgg attttgtccg tctggttgaa aaattaggtt
     9121 tccagcctgt gttactgcca ttttcgccaa atggcaaagc ccagcatatc aaaggctttc
     9181 ttaatcgttt tgcgaagacg gcgaaaaaga cggcggattt cctcaaccgt atggctaagc
     9241 ttgggatgcc aatggtgggc gtcgatccgg cgctggtact ttgttatcgc gatgaatata
     9301 aactggcgct gggcgaggaa cgtggcgagt ttaacgtctt acttgcgaat gaatggctgg
     9361 caagcgcact tgagtcacat ccagtggcta cagtcagcgg tgaatcatgg tatttctttg
     9421 gtcactgtac cgaggttacc gctttgccgg gcgcgccagc acaatgggcc gcgatatttg
     9481 cccgttttgg cgcgaaactg gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt
     9541 acggacatga agcgaaaaac catgaaaatt cgctcgggat ctatgagtta tcctggcatc
     9601 aggctatgca gcgactgccg cgtaaccgct gtctggcgac cggatattcc tgccgtagcc
     9661 aggtaaaacg ggttgaaggc acgggggtac gccatcctgt gcaggcttta ctggagatta
     9721 ttaaatgata tggaaacgga aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa
     9781 catggtgggg ttcctggata ttcgctttga acatattggt gatgacaccc ttgaagcgac
     9841 aatgccagta gactcgcgga caaagcagcc tttcgggttg ctgcatggag gagcatccgt
     9901 ggtactggcc gaaagtatcg gttccgttgc cggttattta tgtaccgaag gtgagcaaaa
     9961 agtggttggt ctggaaatca atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg
    10021 cggcatatgt aaaccgttgc atctcggttc gcgtcaccag gtctggcaga ttgaaatctt
    10081 cgatgagaaa gggcgtttgt gctgttcgtc acgattgacg accgccattt tgtgacgttg
    10141 tcaccagaaa agtgtgacgc atattgcttt tggtcattat ctgcacagta aacggtggta
    10201 tactgtgcag gtcttgtcta aaagcgagga tgctatgtct actcaacttg atcccaccca
    10261 gctggccatt gaatttttac gccgtgacca gagcaatctt tcacctgccc aatatctcaa
    10321 acgtctgaaa caactagaac ttgaatttgc tgacctgctt actctctctt ctgctgaatt
    10381 aaaagaagaa atctactttg cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc
    10441 aagttcgcca aatcgacctg gattaacctc cgtccaggtt cgatgacctt cttaatactg
    10501 cttatctacc gcctgatatt gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc
    10561 gggttagctt tatgagatta ttcttatcgc cccttcaaga gctaagccac tgagagtgcc
    10621 ggagataagc gccggatggg gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt
    10681 ttcttatttc tgaacgctgc acttttttac ctatctctta aatagtcatt ttcaatacaa
    10741 ctttttcttt ttcatcacag ccttaatgct ccttagataa taaccattat ctaacaatga
    10801 gatacctaat tcttagcgtg cctgttaacc cacacatcag gggctatgct tattaaaagc
    10861 aggcaaaaag catgttttac ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat
    10921 tatcactaac atgctgttat acgctgaaag cgatgaagtg aggtaaatag atggacatgc
    10981 attcaggaac ctttaaccca caggatttcg cctggcaagg cttaacgctg acacccgcag
    11041 cggcgataca catccgcgag ctggtggcaa agcagccagg tatggtcggc gtgcgcttag
    11101 gtgtgaagca aacgggctgc gcgggctttg gctatgtgct cgacagtgtt agcgagccgg
    11161 acaaagacga tctgctgttt gaacacgacg gcgcgaagct gtttgtcccg ctgcaatcga
    11221 tgccgtttat tgatggcacg gaaatcgatt tcgttcgtga aggacttaat cagatattca
    11281 aatttcacaa ccctaaagcc cagaatgaat gtggctgtgg cgaaagcttt agggtatagg
    11341 cggtactatg tctcgtaata ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc
    11401 gctgaattat aaagaaggat tcttcaccca gttagccacc gatgagctgg caaaggggat
    11461 aaacgaagag gtggtgcgcg caatttcggc gaagcgtaat gagccggagt ggatgctgga
    11521 gtttcgtcta aacgcctatc gcgcatggct ggagatggaa gaaccgcact ggttgaaagc
    11581 gcactacgac aagctgaatt atcaggatta cagctactac tcagcaccat cgtgcggtaa
    11641 ttgtgacgac acttgcgcgt ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt
    11701 tttaagtaaa gaggtggagg cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa
    11761 agaggtggcg gtggatgcca ttttcgactc tgtttcggtt gccaccactt atcgcgaaaa
    11821 actggcggag cagggaatta ttttctgttc ctttggtgag gcgatccacg atcacccgga
    11881 actggtgcgt aaatatctcg gcaccgtggt gccggggaat gacaacttct ttgcagcact
    11941 taatgcggcg gtagcctctg acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc
    12001 gatggaactt tccacctatt ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac
    12061 aattctggtg gccgacgaag acagctacgt cagctacatt gaaggctgtt ccgctccggt
    12121 gcgtgacagc tatcagttgc acgcggcagt ggtggaagtc atcatccata aaaacgccga
    12181 ggtgaaatat tccacggtac aaaactggtt ccctggcgat aacaacaccg gcggtattct
    12241 caacttcgtc accaagcgtg ctttgtgcga aggcgaaaac agcaaaatgt catggacgca
    12301 atcagaaacc gggtcagcga ttacgtggaa atatcccagc tgcattttgc gcggcgataa
    12361 ctccattggt gagttttact cagtggcgct gaccagcggt catcagcaag cggataccgg
    12421 caccaagatg atccacatcg gtaaaaacac caaatcgacc attatctcga aagggatctc
    12481 tgccggacat agtcagaaca gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa
    12541 tgcgcgcaat ttcactcagt gcgactcaat gctgattggc gctaattgtg gggcgcatac
    12601 cttcccgtat gtcgaatgtc gcaataacag cgcacaactg gagcacgagg caacgacatc
    12661 acgtattggt gaagatcaac tgttttactg cctgcaacgc gggatcagcg aagaagacgc
    12721 catctcgatg attgttaacg gtttctgcaa agacgtgttc tcagaactgc cgctggaatt
    12781 tgccgttgaa gcacaaaaac tcctcgccat cagtcttgaa cacagcgtcg gataaggaat
    12841 aaacatgtta agtattaaag atttacacgt cagcgtggaa gataaagcta tcctgcgtgg
    12901 attaagcctc gacgttcgtc ccggcgaagt tcacgccatt atggggccaa acggttcggg
    12961 caaaagtact ttatcggcaa cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac
    13021 ggttgagttc aaaggcaaag atttgcttgc actgtcgccg gaagatcgtg caggcgaagg
    13081 catctttatg gccttccagt atccggtgga gattccaggc gtcagtaacc agtttttcct
    13141 gcaaacggca cttaatgcag tgcgcagtta tcgcggccag gaaacgcacg accgctttga
    13201 ttttcaggat ttgatggaag agaaaatcgc tctcctgaag atgccggaag atttattaac
    13261 ccgttcggta aacgttggtt tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat
    13321 ggcggtgctg gaaccggagt tatgcattct tgatgagtcg gactccgggc tggatattga
    13381 cgcattaaaa gtggtcgccg atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat
    13441 cattgttacg cactaccaac gcattctcga ctacatcaag cctgattacg ttcatgtgct
    13501 atatcaggga cgaattgtga aatccggcga tttcacgttg gtcaaacaac tggaggagca
    13561 gggttatggc tggcttaccg aacagcagta atgcgctgca acagtggcat cacttgtttg
    13621 aagctgaagg gacaaaacgc tcaccgcaag cacagcagca tttacaacaa ttgctgcgta
    13681 ccggactgcc gacacgtaaa catgaaaact ggaaatatac gccgctggaa gggctgacca
    13741 atagtcagtt tgtcagcatt gcgggagata tatccccaca gcagcgtgat gccttagcgt
    13801 taacgttaga cgccgtgcgg ctggtgtttg tcgatggacg ttacgtgccc gcgctgagcg
    13861 atgcaattga aggcagcgga tatgaagtga gcattaacga cgaccgtcag gggttacccg
    13921 acgctattca ggcggaagtg tttctgcatt tgacggaaag cctggcacaa agcgtgacgc
    13981 atatcgccgt gaagcgcggt caacggccgg caaagccatt gctgttaatg catatcaccc
    14041 agggcgtggc aggtgaagag gtgaacactg cccattaccg gcatcatctg gatctggcgg
    14101 aaggtgccga agcaacggtg atcgaacatt ttgtcagcct taatgatgct cgtcatttta
    14161 ccggggcacg gttcactatc aatgtcgcag cgaacgccca cttgcagcat atcaagctgg
    14221 cgtttgaaaa cccggtcagt caccactttg cccataacga tttgttgctg gctgacgatg
    14281 ccaccgcatt tagccatagt ttcctgctgg gtggcgcagt gttacgacac aacaccagta
    14341 cgcaactcaa tggcgaaaac agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa
    14401 acgaggtgtg tgatacccgt acctggctgg aacacaataa aggtttttgt aacagccgac
    14461 agttgcacaa aactatcgtc agcgacaaag gccgcgcggt atttaacggt ttgatcaacg
    14521 tcgcgcagca cgccatcaaa acggatggtc agatgaccaa caataatctg ctgatgggca
    14581 aactggcgga agtggataca aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca
    14641 gccacggcgc gacggtgggg cgtattgatg atgaacagat gttctatctg cgctcgcgcg
    14701 ggatcaatca gcaggatgcc cagcagatga tcatttacgc ctttgctgcc gaactgacgg
    14761 aagcactgcg tgatgagggg tttaaacagc aggtgctggc ccgaatcggt caacggctgc
    14821 caggaggtgc aagatgactt tttccgtcga caaagtgcgg gccgactttc cggtgctttc
    14881 gcgtgaggta aacggtttgc cgctggctta tctcgacagc gccgccagtg cacagaaacc
    14941 gagccaggtg attgacgccg aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg
    15001 cggtattcat accttaagcg cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc
    15061 atcgctgttt attaatgccc gttcggcgga agagctggtg ttcgtccgcg gcacgacgga
    15121 agggatcaat ctggtcgcca atagctgggg caacagcaat gtgcgggcgg gcgataacat
    15181 catcatcagt cagatggagc accacgctaa cattgtcccc tggcagatgc tttgcgcacg
    15241 cgttggcgca gagctgcgtg tgatccctct caaccccgac ggtacgctgc aactggagac
    15301 gctgcctacg ctgtttgatg agaaaactcg cctgctggca attactcatg tctccaacgt
    15361 gcttggcaca gaaaatccac tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa
    15421 agtgctggtg gatggcgctc aggcggtgat gcatcatccg gtggatgttc aggcgctggg
    15481 ttgcgacttt tacgtgttct ccgggcataa actgtatggc cccaccggaa ttggcattct
    15541 ttatgtgaaa gaagccttgt tgcaggagat gccgccgtgg gaagggggcg gttctatgat
    15601 cgccaccgtc agcctgagtg aaggcactac ctggaccaaa gcaccatggc ggtttgaagc
    15661 cggtacaccc aataccgggg gcatcattgg tcttggcgcg gcgctggagt atgtttcggc
    15721 gctggggctt aatagcatag ccgagtatga acagaatctg atgcattatg cgctatcaca
    15781 gctggaatct gtaccggatc tcactctcta tggccaacaa aacaggcttg gcgttattgc
    15841 ttttaatctc ggtaaacacc acgcctatga tgttggcagt tttctcgata attacggcat
    15901 tgctgtgcgt accggacatc actgcgctat gccattaatg gcctattaca acgtccctgc
    15961 gatgtgtcgg gcgtcgctgg ccatgtataa cacccatgaa gaagtggatc gtctggtgac
    16021 cggcctgcag cgtattcacc gtctgctggg ataacaggga ggcactatgg ctttattgcc
    16081 ggataaagaa aaattgctgc gtaatttttt acgctgcgcc aactgggaag agaaatatct
    16141 ctacattatt gagctgggcc agcgtctgcc agaattacgc gccgaagacg gaagcccaca
    16201 aaatagcatt cagggctgcc agagtcaggt gtggattgtc atgcgccaga atgcgcaggg
    16261 aattattgaa ttgcagggcg acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt
    16321 ctttattctc tacgatcaga tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg
    16381 gtttgaaaaa atggcgctca cccaacatct caccccatct cgttcacaag gtctggaagc
    16441 gatgattcgc gcaattcgcg ccaaagccgc tgcacttagc taaactaaag ggacagcttt
    16501 catccggttg gctcacattg ccggatggtc aatgtttaca ctgacctgca caggagtgtt
    16561 ttggtttcag gtgaacataa gcaccatccg acgtttcagc gtgagtctcc ggcaaataca
    16621 ggaggtttac aatgaaacgc gcgtctttgc ttacactcac gcttatcggc gcttttagcg
    16681 ccatccaggc tgcctgggcg gttgattatc cgctaccacc aaccggaagc cgactggttg
    16741 ggcaaaatca aacgtatacg gtgcaagaag gggataaaaa ccttcaggct attgcccgac
    16801 gttttgatac tgcggcaatg ttgatccttg aagccaataa cactatcgcc ccggtgccaa
    16861 aacctggtac gacgataact attccttcac aactgttatt acctgatgca ccgcgtcagg
    16921 ggattatcgt taaccttgca gagctgcgcc tttattatta tccgccggga gaaaatattg
    16981 tgcaggttta tccaataggt attggattgc aggggctgga aacgccggtg atggaaacgc
    17041 gtgttgggca gaaaatccct aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt
    17101 cgctggagcg tggcattaaa ttaccgccag tcgttcctgc cggaccaaat aacccgctag
    17161 gacgttacgc actgcgcctc gcgcatggta atggcgaata cctcattcat ggcaccagtg
    17221 cgccggacag cgttggtttg cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata
    17281 ttaaagcctt gttctccagc gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg
    17341 tgaaatattc cgtggaacct aacgggatgc gttatgttga agtacatcga ccactatcag
    17401 cagaagaaca gcaaaacgtt cagacaatgc catacacact gccagcaggc tttacgcaat
    17461 ttaaagacaa taaggctgta gatcagaagt tagtcgataa agcgttgtat cgtcgggcag
    17521 ggtatccggt ttcggtgagc agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag
    17581 agtcagcgca gaatggtgaa ccagagcaag ggaatatgtt acgcgcgacg cagtagtggt
    17641 aaatagcaga caaaaaaaat ggcgcacaat gtgcgccatt tttcacttca caggtactat
    17701 tacttgcggt atttagtagc catgttgtcc agacgctggt tagcacgagc tgcgtcatct
    17761 ttagcagcct gaacgtcgga acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta
    17821 gcgttcagag tctgaacgtc agaagacagc tgatcgattt tagcgttgct ggagcaacct
    17881 gccagcagag tagaacccag gattaccgcg cccagtacca gtttagtagc tttcattatt
    17941 aataccctct agattgagtt aatctccatg tagcgttaca agtattacac aaagtttttt
    18001 atgttgagaa tatttttttg atgggaaggc acttattttt gatcgttcgc tcaaagaagc
    18061 atcgaacgct gggttttaga taaaaaaatt gagagaaaac aggatgcttc catcggattc
    18121 atcttaaata aagtgagatt atatagtgaa atccgatt
//
LOCUS       DAJTYG010000075        17460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTYG010000075 DAJTYG010000000
VERSION     DAJTYG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.075"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            564..1334
                     /locus_tag="OO568_002780"
     CDS             564..1334
                     /locus_tag="OO568_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6642070.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="OO568_002781"
     CDS             1325..1798
                     /locus_tag="OO568_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6642071.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGTLSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="OO568_002782"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="OO568_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6642072.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="OO568_002783"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="OO568_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6642073.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGVIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="OO568_002784"
     CDS             3233..3727
                     /locus_tag="OO568_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6642074.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="OO568_002785"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="OO568_002785"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6642075.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO568_002786"
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO568_002786"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR6642076.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFVISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7571..8484
                     /locus_tag="OO568_002787"
                     /pseudo
     CDS             7571..8484
                     /locus_tag="OO568_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            8699..10060
                     /locus_tag="OO568_002788"
     CDS             8699..10060
                     /locus_tag="OO568_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6642077.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="OO568_002789"
     CDS             complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="OO568_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR6642078.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="OO568_002790"
     CDS             complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="OO568_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6642079.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12610..13899
                     /locus_tag="OO568_002791"
     CDS             12610..13899
                     /locus_tag="OO568_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6642080.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13896..15362
                     /gene="hpaE"
                     /locus_tag="OO568_002792"
     CDS             13896..15362
                     /gene="hpaE"
                     /locus_tag="OO568_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6642081.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLTDVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATERNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKLSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15364..16215
                     /gene="hpaD"
                     /locus_tag="OO568_002793"
     CDS             15364..16215
                     /gene="hpaD"
                     /locus_tag="OO568_002793"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6642082.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16225..16605
                     /locus_tag="OO568_002794"
     CDS             16225..16605
                     /locus_tag="OO568_002794"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6642083.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16673..>17460
                     /gene="hpaH"
                     /locus_tag="OO568_002795"
     CDS             16673..>17460
                     /gene="hpaH"
                     /locus_tag="OO568_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6642084.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSI"
BASE COUNT         4297 a         4626 c         4375 g         4162 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgttt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tggggtctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtacgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggtg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctaggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaggcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact aaatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccagcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaataacga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg cttcagctgc cgacaatatc ttttttgatc aatcgcttga gatcatctca
     8281 gtcatctttc attttcacta tcaatgggac gaaagcgatc ttaaacagcg caatatcatt
     8341 gccgtcgatg aacatatgac cattctcagc gctctgattt gccgcagtga ccttgatgcc
     8401 acccttgcgc tccgtaacca tttgaattca gctaaacaat caatgattcg atccattaat
     8461 gagaacacgc gttatgctca ttaagtacgg attaaaaaca gcaaactatt agcgggtgca
     8521 cggttttatc aaaagataaa taccgggaaa ttcccgctta cctatgctca caatcagtcg
     8581 actaaaaatg tttttgtgag cggtaataaa gtcctaaaac tttaacctgc cgttaatgtc
     8641 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agtccggcgt
     8701 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8761 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8821 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8881 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8941 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     9001 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     9061 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     9121 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     9181 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgcggt
     9241 aagcccaccg attctggcgg caatgatgct ggtgatgggc tggcgcggga tgtttattac
     9301 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     9361 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     9421 ccgagatccg ctcaattttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     9481 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc
     9541 tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat
     9601 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     9661 caaaggggga atggctccga ttaaaagccg taagatctgc attattaccg ggatgttctg
     9721 ttctgccgcc tttacgctga tagtaccaca agcgacaaca tccatgatgg cggttctgct
     9781 gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9841 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9901 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9961 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
    10021 cctggttcgc cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
    10081 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
    10141 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
    10201 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
    10261 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
    10321 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10381 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
    10441 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10501 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctacgc tgggccagat
    10561 tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca cgcgctgctt
    10621 caaccgccgc gttcaaagcc agaatattgg tctggaaggt aatgccgtca attacgctga
    10681 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10741 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taatgccaga tggctggcct
    10801 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10861 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag
    10921 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10981 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11041 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11101 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11161 ccagtacgac gatcatcacg cccacaagaa tccacatcgc ctggctgtag gaggcattgt
    11221 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11281 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11341 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11401 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11461 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11521 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11581 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    11641 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaaaaac agaccgcctg
    11701 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11761 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11821 aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag
    11881 atactaaaaa gttattcatt atcgcccggg atgtcttctt gtcgggaatt gcccagagca
    11941 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12001 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12061 ttggtcagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12121 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12181 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12241 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12301 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    12361 aaatcattaa tatagaaaca attaatcatc acgttaccgg gactgagctg cccccgcaat
    12421 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12481 tctttaaatg taaatagttt gttaattaga tcacatttat atcacttact tttgccaaac
    12541 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tcgaatccga
    12601 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12661 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12721 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12781 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12841 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    12901 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12961 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    13021 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    13081 agcgccctga gcgaatttgc cacgctgaat ccaggcgatg ctattctgct cggcacgcca
    13141 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    13201 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    13261 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13321 gaactggaat ttaagccacc ggaagagccg ctggtgttcc tgaaagcgcc gaataccctc
    13381 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    13441 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    13501 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    13561 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    13621 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13681 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13741 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13801 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13861 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13921 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13981 tgctgacgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14041 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14101 gtctgggcga tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca
    14161 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    14221 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14281 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggtgtcg ccgtggaacg
    14341 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14401 tgctgaagat gtcggaactc tccccgctga ccgccaaccg cttgggtgag ctggcgctgg
    14461 aagctggtat tccggcgggc gtgctaaacg tggtacaggg ctacggcgca accgcaggcg
    14521 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    14581 agcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    14641 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    14701 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcaacaga
    14761 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg
    14821 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14881 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14941 ataaactgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    15001 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    15061 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    15121 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15181 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcgc cagccatttg
    15241 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15301 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15361 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
    15421 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    15481 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    15541 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15601 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    15661 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15841 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15901 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15961 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    16021 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16081 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16141 gtggagttta ttaccgaact gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16201 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    16261 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    16321 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    16381 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    16441 agtctggaaa gccgtcagga agtgggcgaa atgctgtttg atctgattaa aacgcacttc
    16501 gcctcactga tggagagccg ttacctagcg ctttcatttg agattgcaga gctgcacccg
    16561 acgttgaatt tcaaacagaa caacgtacac gcgttgttta aataaatttg caccagcttc
    16621 caggccggat gataaggcgc tgccgcatcc ggcaatgcca caggatatcg ctatgttcga
    16681 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
    16741 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtgca
    16801 gcgcgaatgg gttcgcctga aaatcgccga aggtcgcacg ctgaaaggcc acaaaatcgg
    16861 tctgacctcg aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggtgcact
    16921 gctggacgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
    16981 gcgcattgaa gtggagctgg cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac
    17041 gctgttcgac gtttacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
    17101 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtattcg acaccatttc
    17161 cgataacgcc gccaacgccg gagtgatcct cggaggtcgt ccgatcaaac cagacgaact
    17221 ggatctgcgt tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt
    17281 cgccgctggt gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc
    17341 accgtatgac gtacaactgg aagccgggca aatcattctc ggtggttcgt tcacccgccc
    17401 ggttccggcg cgtaagggcg acaccttcca cgtcgattac ggcaacatgg gctccattag
//
LOCUS       DAJTYG010000076        17300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTYG010000076 DAJTYG010000000
VERSION     DAJTYG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.076"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..599)
                     /locus_tag="OO568_002796"
     CDS             complement(<1..599)
                     /locus_tag="OO568_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6642085.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHKVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPRTKWTANKVARYLYISVSTLHRRLA"
     gene            complement(609..1160)
                     /gene="ybcL"
                     /locus_tag="OO568_002797"
     CDS             complement(609..1160)
                     /gene="ybcL"
                     /locus_tag="OO568_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6642086.1"
                     /translation="MKKLVVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1329..1661)
                     /gene="emrE"
                     /locus_tag="OO568_002798"
     CDS             complement(1329..1661)
                     /gene="emrE"
                     /locus_tag="OO568_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6642087.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2006..2320)
                     /gene="fliE"
                     /locus_tag="OO568_002799"
     CDS             complement(2006..2320)
                     /gene="fliE"
                     /locus_tag="OO568_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6642088.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAAIDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2534..4192
                     /gene="fliF"
                     /locus_tag="OO568_002800"
                     /pseudo
     CDS             2534..4192
                     /gene="fliF"
                     /locus_tag="OO568_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            4185..5180
                     /gene="fliG"
                     /locus_tag="OO568_002801"
     CDS             4185..5180
                     /gene="fliG"
                     /locus_tag="OO568_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6642089.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVNDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5173..5859
                     /gene="fliH"
                     /locus_tag="OO568_002802"
     CDS             5173..5859
                     /gene="fliH"
                     /locus_tag="OO568_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6642090.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQVHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAALGVV"
     gene            5859..7160
                     /locus_tag="OO568_002803"
     CDS             5859..7160
                     /locus_tag="OO568_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HCR6642091.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAAYATRIAEDFRDRGQHVLLIMDSLTRYAMAQREIALAIGEPPATKGYPPS
                     VFAKLPALVERAGNGISGGGSITAFYTVLTEGDDQQDPIADSARAILDGHIVLSRRLA
                     EAGHYPAIDIEASISRAMTALISEQHYARVRTFKQLLSSFQRNRDLVSVGAYAKGSDP
                     MLDKAIALWPQLEGYLQQGIFERADWEASLQGLERIFPTVS"
     gene            7179..7622
                     /gene="fliJ"
                     /locus_tag="OO568_002804"
     CDS             7179..7622
                     /gene="fliJ"
                     /locus_tag="OO568_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCR6642092.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7619..8746
                     /gene="fliK"
                     /locus_tag="OO568_002805"
     CDS             7619..8746
                     /gene="fliK"
                     /locus_tag="OO568_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6642093.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRMHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8851..9315
                     /gene="fliL"
                     /locus_tag="OO568_002806"
     CDS             8851..9315
                     /gene="fliL"
                     /locus_tag="OO568_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6642094.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9320..10324
                     /gene="fliM"
                     /locus_tag="OO568_002807"
     CDS             9320..10324
                     /gene="fliM"
                     /locus_tag="OO568_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6642095.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10321..10734
                     /gene="fliN"
                     /locus_tag="OO568_002808"
     CDS             10321..10734
                     /gene="fliN"
                     /locus_tag="OO568_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6642096.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILNN
                     GYLIAQGEVVVVADKYDVRITDIITPSERMRRLSR"
     gene            10737..11102
                     /gene="fliO"
                     /locus_tag="OO568_002809"
     CDS             10737..11102
                     /gene="fliO"
                     /locus_tag="OO568_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6642097.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            11102..11839
                     /gene="fliP"
                     /locus_tag="OO568_002810"
     CDS             11102..11839
                     /gene="fliP"
                     /locus_tag="OO568_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR6642098.1"
                     /translation="MRRLFSVAPVLLWLIMPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11849..12118
                     /gene="fliQ"
                     /locus_tag="OO568_002811"
     CDS             11849..12118
                     /gene="fliQ"
                     /locus_tag="OO568_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6642099.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12127..12912
                     /gene="fliR"
                     /locus_tag="OO568_002812"
     CDS             12127..12912
                     /gene="fliR"
                     /locus_tag="OO568_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR6642100.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFIDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            13197..13820
                     /gene="rcsA"
                     /locus_tag="OO568_002813"
     CDS             13197..13820
                     /gene="rcsA"
                     /locus_tag="OO568_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6642101.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13864..14052)
                     /gene="dsrB"
                     /locus_tag="OO568_002814"
     CDS             complement(13864..14052)
                     /gene="dsrB"
                     /locus_tag="OO568_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6642102.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14215..14442
                     /gene="yodD"
                     /locus_tag="OO568_002815"
     CDS             14215..14442
                     /gene="yodD"
                     /locus_tag="OO568_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6642103.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14740..15555
                     /locus_tag="OO568_002816"
                     /pseudo
     CDS             14740..15555
                     /locus_tag="OO568_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(15552..17246)
                     /gene="yedQ"
                     /locus_tag="OO568_002817"
     CDS             complement(15552..17246)
                     /gene="yedQ"
                     /locus_tag="OO568_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCR6642104.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLIFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLARVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
BASE COUNT         4362 a         4393 c         4446 g         4099 t
ORIGIN      
        1 gccagacggc gatgcaatgt ggaaacagaa atgtagagat atctcgctac cttgtttgct
       61 gtccattttg ttctaggttc ggatagcatt aggttataac aacgacttat taatgattgt
      121 ttactatctg ataaaagtaa acaattaaca tgatgtgctc ctaatgaaag tagaacgccc
      181 attgctaagt gttcctgaat ttgcgttgag aagcctcggg aaatagatgt ttttagttgc
      241 tcccaacaat atattaactc ggggttctga ggtaagcaga aacctgtcgt gttacgaatt
      301 tgatcagtta tcgcataaaa tttttgaaac ttttcaatta gatcaatggg ataataaagc
      361 atttctgcaa gataaagacc tgcttctgga taattcacaa tataaaattc atatccacag
      421 ggaaataata ttatctgatt attatcaaca gttagactat gcgtctccca attgataact
      481 ttttttccct gacggatacg acataaagct ggcataagag gcttaacctt gtgaatctca
      541 tgatgtttat gcatccgtat ttcttcaatc tttaggttag acttatcttt tgccagcata
      601 ctcttaccct actttatctc ataaactggt gttatctcag cggttgcaat tttattggca
      661 ttaagcatat aaccaactaa cgctccgctg gaattagaat ccacaggaat cttatcagtt
      721 tttagagccc atactttaaa ctggtaatga tggggtttat ctcctttggg aggacatgct
      781 ccaccaaatc ctgcataacc aaaatcattt cggccttgaa cagcaccagt cggaagtttt
      841 gtaccatcac gtcttcctgc atcagtgggc aaatatgtta cagttgctgg aatattagca
      901 acagtccagt gccaccatcc actgcctgta ggagcatctg gatcatacac agttacggca
      961 aagcttttgg tgccttcagg agcaccagac caggttaatg agggcgatgt attaccacct
     1021 tcacacccaa atccagaaaa tacatgagac gttgtaagtt gctctcctgt ttttatttca
     1081 ttactagtga cctgaaatgc tgcggcctgc gcagaaaatg ttatgaatgc caatacactt
     1141 gaaacgacaa gttttttcat aaaaacctca ttgttgtgac ctatcgttat tttatttgat
     1201 gtgccttgat ctcattatgc ataaaggcac aatgatcgcg caaatgcaat cataattata
     1261 aaggtgcccc gaacattagc cataatatgc acaaatcaga ttcctgttat ctcaaggaga
     1321 attatttttt aatgcggtgc acttcgtgac aacaagttaa tgactaacac accggcacaa
     1381 atcaacatca tgcctatgat agctggcagg tccagtcgtt gaccaaaaaa tccccaggaa
     1441 agcaaactaa tcagaacgat accgactcct gaccagatag cataagcaat ccctgtaggt
     1501 atataagcca gcgtctgagc taataaccag aatgatgcac aatagcaaat aattgtgcca
     1561 acagagggcc ataaccgtgt aaacccctct gaaaacttca ttaaggttgt accaatgacc
     1621 tctgcaagta ttgcgccacc aagataaata taagggttca tagcatattc tttcctgttc
     1681 atactggaga gaattgtact acagtttgaa cacaactcac cgttttcatc ctagtgtatg
     1741 tgtatccaag gggctctggc gtggcaagag ataaaggcag gaacaataat gatcagaagg
     1801 aaccccaggc taacgaatga gttcactctt cctgggagca aagcgaagac aaaacatgtt
     1861 atcgcgttta cggatcgtgg atggcagaaa acaaccagga tcaggtatcc attttaaacc
     1921 agaaattgag tgagtttgcc ccatccatgc cccacgctgt gggatcggat ataattaaac
     1981 aagcatagtc aacaggttac acaacctaca cctgcataga catcacttcc tgatacgccg
     2041 ccaccagctt attacgcacc tgaatcccca tttgcataga aactgaggct ttttgcatat
     2101 cggtcatcac atcgtttagc gccacgccgg gttcaccgag ggtgaatttt tctgcctgcg
     2161 tgcgggcgac tgtttgtgta tcgcttatgc gatcgatcgc ggcgtgcagc tgcccggcaa
     2221 aactaatggt cggttgcggc agtgattcct gcgcacgcgc actcatcgcc gtagcctgta
     2281 actggctgat aaccccttca atcccctgta tcgctgacat tttcgtctcc cggataattt
     2341 ctggtaacaa agcctaccag taagtcaata agacaaaggc gctaaatagc aacaaaaaaa
     2401 cgggtttatt ggcggataga aaaaacgaaa gcacaaataa tgggagcgtc aatttttcga
     2461 gtttgctgac ccgagagtga gtcttgttcc actttgccaa taacgccgtc cataatcagc
     2521 cacgaggtgc gcgatgaatg cgactgcagc ccagacaaaa tctcttgagt ggcttaatcg
     2581 cctgcgtgcg aatccgaaaa ttccattgat tgttaccggt tccgcggcag tggcggtcat
     2641 ggtcgcactg atcctgtggg cgaaagcccc cgactaccgc acattattca gcaatctttc
     2701 cgatcaggat ggtggcgcaa ttgtcagcca actgacgcaa atgaatattc cttaccgctt
     2761 cagcgaagcc agcggcgcta ttgaggttcc ggcagataaa gttcacgaac tgcgcctgcg
     2821 cctggcgcaa caaggtttgc ccaaaggcgg cgcggtcggt ttcgaactgc tcgatcagga
     2881 aaagtttggt atcagccagt tcagcgaaca ggtgaattat cagcgggcgc tggaaggcga
     2941 gctttctcgt accatcgaaa ctatcggccc ggtaaaaggg gcgcgcgtac atctggcaat
     3001 gccgaaaccg tctttattcg tccgtgaaca aaaatcccct tctgcatcgg tgacggtaaa
     3061 tctgttaccc ggccgcgcac tcgatgaagg gcaaattagc gctattgtac atctggtttc
     3121 cagcgcggtt gctggtctgc cgcctggaaa cgtcacgctg gtggatcagg gcgggcatct
     3181 gttaacccag tccaatacca gcgggcgcga tcttaatgac gctcagttga aatatgccag
     3241 cgatgtcgaa ggccgtattc agcggcgtat tgaagcgatc ctgtcgccta ttgttggtaa
     3301 cggtaatatt cacgcctagg tcacggcgca actggacttc gccagtaaag aacaaacgga
     3361 agaacagtat cgccctaacg gtgatgaatc tcatgcggcg cttcgttcac gccagcttaa
     3421 tgagagcgag caaagcggtt ccggttatcc gggcggcgta ccgggggcgt tgtcgaatca
     3481 accggcacct gcgaataacg cgccaatcag cacgcctccg acaaatcaaa ataaccgcca
     3541 gcagcaggcg agcaccacca gcaatagtgg gccgcgtagc acacagcgga atgaaaccag
     3601 taactacgaa gtcgatcgca ccattcgtca taccaaaatg aacgtgggcg atgtgcaacg
     3661 tctgtcagtc gcggtagtgg tgaattacaa aaccttgcca gacggtaaac cattgcctct
     3721 cagcaacgaa cagatgaagc aaattgaagc tctgacccgc gaggcgatgg gcttttctga
     3781 aaaacgcggt gattcgctca atgttgttaa ctcgccgttc aatagcagtg acgaaagcgg
     3841 cggagcactg ccgttttggc aacagcaagt gtttatcgat cagttgctgg ctgccggtcg
     3901 ctggttgctg gtactgctgg tggcgtggtt gctgtggcgg aaagcggtac gcccgcagct
     3961 aacacgtcgc gccgaggcgg tgaagactgt gcagcaacag gcgcaggccc gcgaggaagt
     4021 ggaagatgcg gtggaagtcc gtctgagcaa agacgaacaa ctccaacaac ggcgcgctaa
     4081 ccaacgactg ggggcagaag tcatgagcca gcgtatccgt gaaatgtctg ataacgatcc
     4141 gcgcgtggtg gcgctggtca ttcgccagtg gataaataac gatcatgagt aacctgacag
     4201 gcaccgataa aagcgtcatc ctgctgatga ccattggcga agaccgggcg gcagaggtgt
     4261 tcaagcacct ctcccagcgc gaagtgcaaa ccctgagcgc tgcaatggcg aacgtcacgc
     4321 agatctccaa caagcagcta accgatgtgc tggcggagtt tgagcaagaa gctgaacagt
     4381 ttgctgcact gaatatcaac gccaacgatt atctgcgttc ggtattggtc aaagctctgg
     4441 gtgaagaacg tgccgccagc ctgctggaag atattctcga aacccgcgat accgccagcg
     4501 gtattgaaac gctcaacttt atggagccgc agagcgccgc cgatctgatt cgcgatgagc
     4561 atccgcaaat tatcgccacc attctggtgc atctgaagcg cgcccaggcc gccgatattc
     4621 tggcgttgtt cgatgaacgt ctgcgccacg acgtgatgtt gcgtatcgcc acctttggcg
     4681 gcgtgcagcc agccgcgctg gccgaactga ccgaagtact gaatggcttg ctcgacggtc
     4741 agaatctcaa gcgcagcaaa atgggcggcg tgagaacggc agccgaaatt atcaacctga
     4801 tgaaaacgca gcaggaagaa gccgttatta ccgccgtgcg tgaattcgac ggcgagctgg
     4861 cgcagaaaat catcgacgag atgttcctgt tcgagaatct ggtggatgtc aatgatcgca
     4921 gcattcagcg tctgttgcag gaagtggatt ccgaatcgct gctgatcgcg ctgaaagggg
     4981 ccgagcagcc actgcgcgag aaattcctgc gcaatatgtc gcagcgtgcc gccgatattc
     5041 tgcgcgacga tctcgccaac cgtggtccgg tgcgtctgtc gcaggtcgaa aacgaacaga
     5101 aagcgattct gctgattgtg cgccgtcttg ccgaaactgg cgagatggtg attggcagcg
     5161 gcgaggatac ctatgtctga taatctgccg tggaaaacct ggacgccgga cgatctcgcg
     5221 ccaccaccgg cagagtttgt gccaatggtc gagtcggaag aaaccattat tgaagaggcc
     5281 gaacccagcc ttgagcagca actggcgcaa ctgcaaatgc aggtccacga gcagggttat
     5341 caggcgggga ttgccgaagg tcgccagcaa ggtcacgaac agggctatca ggaaggactg
     5401 gcccaggggc tggaacaagg tctggcagag gcgaaggctc aacaagcgcc aattcatgcc
     5461 cggatgcagc aactggtcag cgaatttcaa actacccttg atgcgcttga tagtgtgata
     5521 gcttcgcgcc tgatgcagat ggcgctggag gcagcacgtc aggtgatcgg tcagacacca
     5581 acggtggata actcggcact gatcaaacag atccaacagt tgttgcagca agaaccgtta
     5641 ttcagcggga aaccacagct gcgcgtacac ccggatgatc tgcaacgtgt ggatgatatg
     5701 ctcggcgcaa ctttaagttt gcatggctgg cgcttgcggg gcgatcccac cctccaccct
     5761 ggcggatgta aagtctccgc cgatgaaggc gatctcgacg ccagtgtcgc cactcgctgg
     5821 caagaactct gccgtctggc agcactagga gtggtgtaat gaccacgcgc ctgactcgat
     5881 ggctaaccac gctggataac tttgaagcca aaatggcgca gttgcctgcg gtacgtcgct
     5941 acgggcgatt aacccgcgct accgggctgg tgctggaagc caccggatta caattgccgc
     6001 tcggcgcaac ctgtgtcatt gagcgccaga acggcagcga aacgcacgaa gtagaaagcg
     6061 aagtcgttgg ctttaacggt caacggctgt ttttaatgcc gctggaggaa gtggaaggtg
     6121 tcctgcccgg cgcgcgtgta tatgccaaaa acatttcggc agaagggctg caaagcggca
     6181 agcagttgcc gctcggtccg gcgttattag gtcgtgttct ggacggtagc ggtaaaccgc
     6241 tcgatggcct gcccttcccc gatacgacgg aaaccggtgc gctgattacc ccgccattta
     6301 acccgttgca acgtacaccg attgaacatg tgctggacac cggcgtgcgc ccaatcaatg
     6361 ccctgcttac cgtcggacgc gggcagcgta tggggctgtt tgccgggtcg ggcgttggta
     6421 aaagtgtgct gctggggatg atggcacgtt acacccgcgc cgatgtcatt gtcgtgggtt
     6481 tgattggtga acgtgggcgc gaagtaaaag attttattga gaatatcctc ggtgccgcct
     6541 atgccacgcg cattgccgaa gattttcgcg atcgtggtca gcatgtgttg ctgattatgg
     6601 actccctcac ccgctacgcg atggcccagc gtgaaattgc gctggcgatt ggcgaaccac
     6661 ctgccactaa aggttatcca ccgtcggtgt ttgccaaatt accggcactg gtcgagcgtg
     6721 ccggtaatgg cattagcggc ggcggctcga ttaccgcgtt ttataccgtg ctcaccgaag
     6781 gcgatgacca gcaggacccc attgctgact ccgcgcgggc aatcctcgac ggtcacattg
     6841 tgctgtctcg ccgactggcg gaagccgggc actatccggc tatcgatatt gaagcgtcga
     6901 tcagtcgcgc aatgacggcg ttgatcagtg agcaacatta cgcgcgagtg cgcaccttca
     6961 aacagctgtt gtcgagtttt cagcgtaacc gcgatctggt tagcgtcggc gcgtatgcca
     7021 aaggcagcga tccgatgctc gataaagcca tcgccctgtg gccgcagctg gagggctatt
     7081 tgcaacaagg catttttgaa cgcgcggact gggaagcgtc tctccagggg ctggagcgta
     7141 ttttcccgac agtgtcataa cccaggagat aacggcagat ggcggaacat ggtgcgctgg
     7201 cgaccctgaa agatctggca gaaaaagagg tagaggatgc cgctcgcctg ctgggtgaaa
     7261 tgcgtcgcgg atgtcagcag gcggaagaac agctcaaaat gctgatcgat tatcagaatg
     7321 aatatcgcaa taacctcaat agcgatatga gtgccgggat gaccagcaac cgctggatca
     7381 actatcagca gtttatccag acgctggaaa aagccattac tcagcatcgc cagcaactta
     7441 atcagtggac gcagaaagtt gacattgccc tgaacagctg gcgagaaaag aaacaacgtt
     7501 tgcaggcctg gcagacactg caggaacggc aatccacggc ggcactgctt gcagaaaacc
     7561 gcctcgatca gaaaaagatg gatgagttcg cccagcgcgc cgccatgagg aaacctgaat
     7621 gattcgctta gcgcccttga ttaccgccga cgttgacacc accacattgc ctggcggcaa
     7681 agccagcgat gctgcacaag attttctcac gttgttgagc gaagcattag caggcgagac
     7741 aacaactgac aaagcggccc cccagttgct ggtggcaaca gataagccca cgacaaaagg
     7801 cgagccgctg gtcagcgata ttcttgccga tgcgcaacaa gcggatttac tgatccctgt
     7861 ggatgaaaca cttcctgtca tcaacgacga acaatccaca tcaacaccgt taaccaccgc
     7921 tcagacaatg acgttggctg cggtggctga caaaaatacg acaaaagacg aaaaagcgga
     7981 tgatcttaat gaagacgtca ccgcaagtct gagcgccctt tttgcgatgt tgccgggttt
     8041 tgacaatacg cccaaagtga ctgatgcacc gtcaaccgtg ttaccggcag agaaaccaac
     8101 gctcttcaca aaactgactt ctgcgcaact cactacagca cagcctgatg acgcccccgg
     8161 cacaccagct cagccattaa caccgctggt agcagaagcc cagagtaaag cggaagtcat
     8221 cagcacgcct tcgccggtga ccgctgacgc cagcccgcta atcactccac accagacaca
     8281 gccactgccc accgtcgccg cgcctgtttt gagtgcaccg ctgggttctc acgaatggca
     8341 acaatcattg agccagcata tttcgctgtt cacccgccag gggcaacaaa gtgcagagtt
     8401 gcgtatgcac ccacaggatt taggtgaagt gcaaatttcc ctcaaagtgg atgataacca
     8461 ggcgcagatc cagatgattt caccgcatca gcacgtacgc gccgccctgg aagcagcgct
     8521 gccggtactg cgtacgcagc tggccgaaag tggcattcag ttagggcaaa gcaacattag
     8581 tggcgaaagc tttagtggtc agcagcaggc cgcttcccag caacagcaaa gccaacgcac
     8641 agcaaaccat gaacctctgg cgggggaaga cgacgatacg cttccggttc ccgtctcttt
     8701 acaagggcgt gtaacaggta acagcggcgt tgatattttc gcctaacgtc agaggtagca
     8761 ccgtaatccg cgtcttttcc ccgctttgtt gcgctcaaga cgcaggataa ttagccgata
     8821 agcagtagcg acacaggaag accgcaacac atgactgatt acgcgataag caagaaaagc
     8881 aagcgatcgc tttggatccc gattctggta ttcattaccc tcgcggcctg tgccagcgca
     8941 ggttacagct actggcattc gcatcaggtt gccgctgacg acaaagcgca gcaacgcgtc
     9001 gtgccctccc cggtcttcta tgcgctggat acctttacgg tcaatttggg cgatgcggat
     9061 cgcgtgcttt atatcggcat aaccctgcga ctgaaagatg aagcaacccg ctcgcggctg
     9121 agtgagtatt tgccggaagt ccgtagtcgc ttgctgttac tgttttcacg tcaggatgct
     9181 gccgtactgg cgacagaaga aggcaagaaa aacctgattg ccgagattaa aaccacactt
     9241 tccaccccgc ttgttgccgg gcaaccgaaa caggatgtca ccgacgtgct gtataccgct
     9301 tttattctgc gataacgaca tgggcgatag tattctttct caagctgaaa ttgatgcgct
     9361 gttgaatggt gacagcgaag tcaaagacga accgacagcc agtgttagcg gcgaaagtga
     9421 cattcgtccg tacgatccga atacccaacg acgggttgtg cgcgaacgtt tgcaggcgct
     9481 ggaaatcatt aatgagcgct ttgcccgcca ttttcgtatg gggctgttca acctgctgcg
     9541 ccgtagcccg gacattaccg tcggggccat ccgcattcag ccataccatg aatttgcccg
     9601 caacctgccg gtgccgacca acctgaacct aatccacctg aaaccgctac gcggcacagg
     9661 gctggtagtg ttctcgccga gtctggtgtt tatcgccgtg gataacctgt ttggcggcga
     9721 tggacgcttc ccgaccaaag tggaaggtcg cgagtttacc catactgaac agcgcgtcat
     9781 caaccgtatg ttgaagctgg cgctggaagg ctatagcgac gcctggaaag cgattaatcc
     9841 gctggaagtt gagtacgtgc gttcggaaat gcaggtggaa tttaccaata tcaccacctc
     9901 gccgaacgac attgtggtta acacgccgtt ccatgtggag attggcaacc tgaccggcga
     9961 atttaatatc tgcctgccat tcagcatgat cgagccgcta cgggaattgt tggttaaccc
    10021 gccgctggaa aactcgcgta atgaagatca gaactggcgc gataacctgg tgcgccaggt
    10081 gcagcattca cagctggaac tggtggccaa ctttgccgat atctcgctac gcctgtcgca
    10141 gattttaaaa ctgaaacccg gcgacgtcct gccgatagaa aaacccgatc gcatcatcgc
    10201 ccatgttgac ggcgtcccgg ttctgaccag tcagtacggc actctcaacg gtcagtatgc
    10261 gttacggata gaacatttga ttaacccgat tttaaattct ctgaacgagg aacagcccaa
    10321 atgagtgaca tgaataatcc ggccgatgac aacaacggcg caatggacga tctgtgggct
    10381 gaagcgttga gcgagcaaaa atcaaccagc gaaaaaagcg ctgccgacgc ggtgttccag
    10441 caatttggcg gtggtgatgt cagcggaacg ttgcaggata tcgacctgat tatggatatt
    10501 ccggtcaagc tgaccgtcga gctgggccgt acgcggatga ccatcaaaga gctgttgcgt
    10561 ctgacgcaag ggtcagtcgt ggcgctggac ggtctggcgg gcgaaccgct ggatattctg
    10621 aacaacggtt atttaatcgc ccagggcgaa gtggtggttg ttgccgataa atatgacgtg
    10681 cggatcaccg atatcattac cccgtccgag cggatgcgcc gcctgagccg ttagtgatga
    10741 ataaccatgc tactgtgcaa tcttccgcgc cggtttctgc tgagccactg ctgcaggtga
    10801 gcggcgcact catcgccatt attgccctga tcctcgctgc tgcctggctg gtaaaacggc
    10861 tgggatttgc ccctaaacgc actggcgtta acggtctgaa aattagcgcc agtgcttcgc
    10921 tgggcgcgcg tgaacgggtc gtggtggtcg atgtggaaga tgcacggctg gtgctcggcg
    10981 ttaccgcagg gcaaatcaat ctgctgcata aacttccccc ttcagcacca acggaagaga
    11041 taccgcagac cgattttcag tcggtcatga aaaatttgct taagcgtggc gggagatcct
    11101 gatgcgtcgt ttgttttctg tcgcaccggt ccttctctgg ctgattatgc ccctcgcctt
    11161 cgcgcaactg ccgggtatca ccagccagcc gctacctggc ggtggacaaa gctggtcgct
    11221 cccggtgcag acgctggtgt tcatcacctc gttgacgttc attccagcga ttttactgat
    11281 gatgaccagt ttcacccgca tcattattgt ttttggttta ttgcgtaacg cgctgggaac
    11341 gccctccgcg ccacctaacc aggtattgct ggggctggca ctgtttttga ccttttttat
    11401 tatgtcgcca gtgatcgaca aaatttatgt cgatgcgtac cagccattca gcgaagagaa
    11461 aatctcaatg caggaggcgc tggaaaaagg ggcgcagccg ctgcgtgagt ttatgctgcg
    11521 tcagacccgc gaggcagatt tagggttgtt tgccagactg gcgaataccg gcccgttgca
    11581 ggggcctgaa gctgtaccga tgcgcatttt gctcccggcc tacgtgacca gcgagttgaa
    11641 aaccgcattt cagataggct tcacgatttt catccctttt ttgattatcg acctggtgat
    11701 tgccagcgtg ttgatggcat tggggatgat gatggttccc cccgccacca ttgctctgcc
    11761 ctttaaactg atgctgtttg tactggtgga tggctggcaa ttgctggtcg gttcgctggc
    11821 gcagagcttt tacagctaga gaggcaaaat gacacctgaa tcggtcatga tgatgggaac
    11881 tgaagcgatg aaagtcgcgc tggcactggc agccccgctg ttgctggtag cgctggtcac
    11941 gggccttatc atcagtattt tgcaggcagc cacgcagatt aacgaaatga cgctgtcgtt
    12001 tattccgaaa atcatcgccg tatttatcgc catcattatt gccggaccgt ggatgctcaa
    12061 tctgttgctg gattacgtcc gcaccttgtt cactaacctg ccgtatatca tcgggtagcc
    12121 cgtacaatga tgcaggagac aagcgatcaa tggctttcct ggttaagcct gtacttctgg
    12181 ccgttactgc gcgtgctggc gctcatttct accgcgccaa ttctgagcga acgcagcgtg
    12241 ccgaaacggg taaaactggg tctggcaatg atgatcacgt tcgccattgc cccatcatta
    12301 cctgccaacg atgttcctgt tttttcgttc tttgctctgt ggctggccgt gcagcagatc
    12361 ctgatcggca ttgcgcttgg ttttaccatg caatttgcct ttgccgctgt gcgaaccgct
    12421 ggcgaaatta tcggtctgca aatggggctg tcatttgcga catttatcga tcctggcagc
    12481 catctcaata tgcccgtttt agcgcgcatc atggatatgc tggcattact gctgttcctg
    12541 acatttaacg gtcatttatg gttgatttca ctgctggtcg atacctttca caccctgccg
    12601 attggtagcg aaccgttgaa cagcaatgcg tttctggcac ccaccaaagc agggagtttg
    12661 attttcctta acgggctgat gctggcgtta ccgctcatta ctctgttgct gaccctgaat
    12721 ctggcattag gtttactgaa tcgtatggcc ccgcaattat ccatttttgt tattggattt
    12781 ccattaactc tgactgtcgg catctcttta atggcggcat taatgccgtt aattgcgcct
    12841 ttttgcgaac atttattcag tgaaattttt aatttgctgg ctgatattat tagtgaatta
    12901 ccattaatat aattccgtaa cgtttatcat gctaccctaa ggattatccg aaaaataata
    12961 tctgcgaaca tcttccagga tactcctgta gcgaaatatt tgttttaagc tcactcacat
    13021 atcgcaacat ttactttaag acaattccag gcaaattata caacacttta cgggatagta
    13081 agtccgcctg aaaaattgcg agagtggcgc attaggtgac ccatgttgtt ccgtttagtc
    13141 atgatgaaat attcaggtaa ggggaattat cgttacgcat tgagtgaggg tatgccatgt
    13201 caacgattat tatggattta tgtagttaca cccgactagg tttaaccggg tatctgttga
    13261 gtagaggggt taaaaaaaga gaaatcaacg acattgaaac cgttgatgac cttgccatag
    13321 cttgtgattc acagcgtcct tcagtggtgt ttattaatga ggactgtttc atccacgatg
    13381 cttctaacag tcagcgcatc aagcacatca ttaatcaaca tcccaatacg ttatttatcg
    13441 tttttatggc aattgccaat gttcattttg atgaatatct attggtcaga aaaaatttat
    13501 tgatcagttc taaatcgatt aaaccggaat ctctcgacga tatccttggc gatattctga
    13561 aaaaagagac aacgataacc tcgtttttaa atatgccgac gttatcattg agccgaaccg
    13621 aatcgagtat gttgcgaatg tggatggcag gtcagggaac cattcaaatc tccgaccaaa
    13681 tgaatatcaa agccaagacc gtttcatcgc ataaaggtaa tattaaacgt aagatcaaaa
    13741 cgcataataa acaggttatc taccatgtcg tccgactgac ggataatgtg actaatggta
    13801 tttttgtcaa catgcgctaa cacattctga ctggtggttt cccaccagtc aggctgaata
    13861 agattactct gctttctcaa caaagatacc gtcctgatgc cctgcttcat taaagaacca
    13921 gatgccgagc gggtagtctt ccagcgcaac caggtacatt gtgccttcac taaactcctc
    13981 aactgccagt accactccag gacgacgcgg accgccatcc gttttgactg ttacccgatc
    14041 attcaccttc atcgttttcc tcctgtggct ttgtgccagt gtagaacaat ttcgtttttc
    14101 tggcagcgcc aagcgcgcgc gagtgctgat tttctcgact gtctatactt aagagatgcc
    14161 aggcggactt aacgactggc ggcaacaaca gagtaacggt tgcgaggaaa gatgatgaaa
    14221 accgcaaaag tgtacagcga taccgcaaaa cgtgaggtca gcgtcgatgt cgatgccctg
    14281 ctggcggcga tcaatgaaat tagcgaaagc gaagttcatc gcagccagaa cgattctgaa
    14341 cacgttagcg tcgatggacg tgaatatcat acatggcgtg aattggcgga tgccttcgaa
    14401 ctggatattc atgacttcag cgtctctgaa gtgaatcgtt gaatgcacaa taaaaaaatc
    14461 ccgaccctga gggtgtcggg atgaaacttg cttaagcaag aagcacttaa aaaattcgtt
    14521 acaccaggaa atctgatgtg ttcatcacct tatctgcaat ttttttcgct gacaagaaaa
    14581 tattcgcgct atgaatgatt aacgctactt atgaatagaa atgtgacgcg cgtcacccct
    14641 tggtggctct ggtgtcaacg ggatcgctcc cgaaaaaatc atctttgatt atttgtgctg
    14701 ttttgagaat catcctggta aggggagtac tgcgcgacca tgttttcaat tcaacaacca
    14761 ctactggttt ttagcgatct tgatggcacc ctgctggaca gtcatagtta tgactggcaa
    14821 ccggcagccc cctggctcag ccgtttacac gaagccaata ttcccgtcat tctctgtagc
    14881 agtaaaactt cagcggaaat gctgtacttg caaaaaatgt tggggctaca aggcttaccg
    14941 ctgattgcag agaatggcgc agtgatccag cttgctgaac aatggcagga tatagacggt
    15001 tttccacgca tcatctcagg tattagccat ggcgaaatct gccaggtttt aaatacgcta
    15061 cgcgagaaag aacattttaa attcacgact tttgatgatg tcgacgatgc aaccattgcc
    15121 gaatggacgg gattaagccg tagctaggcg gcgctgacgc agctgcatga ggcgtcggta
    15181 acgctaatct ggcgcgacag tgacgagcgt atggcacaat ttatcgctcg tctgaacgaa
    15241 ctgggcttgc agtttatgca gggtgcgcgc ttctggcacg tactggatgc ctctgccgga
    15301 aaagatcagg ctgccaactg gattatcgcg acctatcaac aattgtcagg cagacgccca
    15361 accacacttg gcctgggcga tgggccaaac gatgcgcctt tactggaggt aatggattac
    15421 gcggtgattg tgaaagggct aaaccgtgaa ggggtacatc tgcatgatga ggatccggcc
    15481 cgcgtctggc gaacgcagcg tgaaggaccg gaaggctggc gtgaagggct ggaccatttt
    15541 ttctccgccc gttaagcgtt atcgctcgcg aatacccgat tgcgcccggc ctgttttgcg
    15601 aggtaaagcc gacggtcggc caatgactgg agttgttcaa aatcataatc accggtttcc
    15661 tcgctgctac ttacccccag cgaggcactg atgcgtatcg ttgtactctt ggcgatcagc
    15721 atctcttttt catttaactt caggcgaata cgttctgcga cctccgcagc ctgcgtcaga
    15781 ttcgcgcctg gcagaatcac acaaaactcc tcaccaccga cccgcccggc aacgtcctgc
    15841 gcacgcaagg aactgctaat taatccggca gcatgagaaa gaacacggtc gcccgcctga
    15901 tgaccaaagc ggtcattaat cgctttaaaa tggtcaagat cgacctgaat gacagaaaaa
    15961 ggatgttggt gcgtctgaca caatttagcg agcggacggg ctttttcgaa cagtgcgcca
    16021 cggttatata aacgcgttaa ggtgtcgtgc cacgcctgcc actgcaacga gctttgcaga
    16081 acatacatgt tgctgaccat ccggcgaatc acataccagg aaaggagtaa catggtggta
    16141 aagagcgccc acagcagggt taatgcaatg ctgatactgc cgaaatcgcc gcgcacgcct
    16201 tcgcttagcg tatggacgcg cgccagcaca ccgtcgaaat ggtccagacg ttcccagcta
    16261 acatagcgac tgtccatacg aatgccgccc cgcgtgtcat gttccattgc ctgcgccagc
    16321 aaggccagtt cacgaggatc aaaaatattc cctgttggat ggtcaggatt ggaagaggtc
    16381 aaaaatatca gcttactgtc atagagctga tactcaccat cgaggttttt atcgatggcg
    16441 tttcttaaaa attgctgcat agtacgcacg ggaatactca tccccagcac gccataccag
    16501 tagttattac tgtctaccgg aacactgacg gtaacctgcg gttcagtatt gctggcgtgt
    16561 tccggttgcg aagtgaacca gcgtaccgcg cggtgacgat tttctcgttg cgaatggccg
    16621 ataaaccagg gttgggtgac atagccgtaa taacgcgttg gtacattgcg cataaacaag
    16681 gtcggctgcg tcgaaacgta aaatccagca cgcgagacat acatcgcctg ttcaaccatc
    16741 gacgaggagt tgtgcgccag tcgcagcaag taaccaactt ccagtgcagc ggtaatttca
    16801 ttgtcgaggc tttcattttc gcgagacagg aaattcccct cgctgactaa tgcatccgat
    16861 acgccattga ctggcagagt gcgccgtcgg ttgagttcga tttgccaggc gtgctcgtcg
    16921 cgatgctgtt cgaactcggt tacggcatta cgcagagagg tgaaatccag tggcgccacg
    16981 agagcttcgc gcatgccatt acgcaaaaag atcagtttgt cgacgttata ctgcaaatgc
    17041 ttatcgagcg cgttggctac gttttccaga tgattacgct ggctggagat ataggcatct
    17101 tccagcacca ccacttcacg ccaggtgagc aaggtggaaa aaagcaatac cacgataaag
    17161 cagagattaa cgacatgacc gggccccagg cggcgtgcga gttttttcaa ccagctctgg
    17221 ttttccattt ttgtctcgtg ctgcaccctg acgactccca acctgctttt ttatgattct
    17281 ggcataaccc ggcaatagcg
//
LOCUS       DAJTYG010000077        17239 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTYG010000077 DAJTYG010000000
VERSION     DAJTYG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.077"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..171
                     /locus_tag="OO568_002818"
     CDS             <1..171
                     /locus_tag="OO568_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR6642105.1"
                     /translation="CSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGA
                     VFGGELTLVQLQ"
     gene            complement(172..363)
                     /locus_tag="OO568_002819"
     CDS             complement(172..363)
                     /locus_tag="OO568_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR6642106.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(468..704)
                     /locus_tag="OO568_002820"
     CDS             complement(468..704)
                     /locus_tag="OO568_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR6642107.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(822..2261)
                     /gene="rsmF"
                     /locus_tag="OO568_002821"
     CDS             complement(822..2261)
                     /gene="rsmF"
                     /locus_tag="OO568_002821"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR6642108.1"
                     /translation="MAQHAVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFGGRVFGASVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPVPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2341..4974)
                     /gene="yebT"
                     /locus_tag="OO568_002822"
                     /pseudo
     CDS             complement(2341..4974)
                     /gene="yebT"
                     /locus_tag="OO568_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4943..6227)
                     /gene="yebS"
                     /locus_tag="OO568_002823"
                     /pseudo
     CDS             complement(4943..6227)
                     /gene="yebS"
                     /locus_tag="OO568_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6357..6854
                     /gene="msrC"
                     /locus_tag="OO568_002824"
     CDS             6357..6854
                     /gene="msrC"
                     /locus_tag="OO568_002824"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6642109.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6951..7649
                     /gene="proQ"
                     /locus_tag="OO568_002825"
     CDS             6951..7649
                     /gene="proQ"
                     /locus_tag="OO568_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6642110.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7669..9717
                     /gene="prc"
                     /locus_tag="OO568_002826"
     CDS             7669..9717
                     /gene="prc"
                     /locus_tag="OO568_002826"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6642111.1"
                     /translation="MSMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLERSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMMAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9909..10790
                     /gene="htpX"
                     /locus_tag="OO568_002827"
     CDS             9909..10790
                     /gene="htpX"
                     /locus_tag="OO568_002827"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6642112.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10836..12209)
                     /locus_tag="OO568_002828"
     CDS             complement(10836..12209)
                     /locus_tag="OO568_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6642113.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12386..13177
                     /gene="kdgR"
                     /locus_tag="OO568_002829"
     CDS             12386..13177
                     /gene="kdgR"
                     /locus_tag="OO568_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6642114.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13320..13559)
                     /locus_tag="OO568_002830"
     CDS             complement(13320..13559)
                     /locus_tag="OO568_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642115.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13718..13861
                     /gene="mgrB"
                     /locus_tag="OO568_002831"
     CDS             13718..13861
                     /gene="mgrB"
                     /locus_tag="OO568_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6642116.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13936..14223
                     /locus_tag="OO568_002832"
     CDS             13936..14223
                     /locus_tag="OO568_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6642117.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14893..15036
                     /locus_tag="OO568_002833"
     CDS             14893..15036
                     /locus_tag="OO568_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6642118.1"
                     /translation="MCGIFSKEVLGKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            15049..15258
                     /gene="cspE"
                     /locus_tag="OO568_002834"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             15049..15258
                     /gene="cspE"
                     /locus_tag="OO568_002834"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6642119.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15424..16233
                     /gene="rlmA"
                     /locus_tag="OO568_002835"
     CDS             15424..16233
                     /gene="rlmA"
                     /locus_tag="OO568_002835"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6642120.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16230..16796)
                     /gene="mntP"
                     /locus_tag="OO568_002836"
     CDS             complement(16230..16796)
                     /gene="mntP"
                     /locus_tag="OO568_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6642121.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGSDDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4555 a         4169 c         4346 g         4169 t
ORIGIN      
        1 tgtagccgct cgcgattagg tgaacgccca aaagggcagg gaattacagg tgctgatcat
       61 ttctggtttg gtcatacacc gttgcgacat cgcgtggata ttggcaacct gcattatatt
      121 gataccggtg ctgtctttgg gggcgaactg actcttgtgc aactgcaata attaaaaatc
      181 accatactcc tgtgctggtc gccagaaacc atctataaaa tcctcaatcg gaaaacaacc
      241 gccatggcgg atccgttgat cgctcataga ataaagacac tgctgttccg tgttgtagac
      301 atccacaaca atatcttcac aaccgccatc caggtagcaa acaaaaagta ccagcgcgaa
      361 catttcatcc ccgaagtgtg gtgccgtacc gttaagttta ggagagattt tacaacgggg
      421 gaataaccag gacaaataac ccgccatgcc gacgggttct ttttggatca ggcaagacgc
      481 ataatccagg catcagattg ttgatcgtaa cgggtacggt aaaggacaga gtgttcgcca
      541 tttagcaggg cagggatact ggtttcagca tcccaggcat ccagttgcat acataaatcg
      601 gggtcagatt tacaaggaat ggttaacgtt cgatcacccg gcgattcacc gtgtaattcg
      661 ccgtcgtcga tttcaaaagc gcctatgcgc acactggttt tcatcttcgt gctctccatt
      721 tagctgctga ttgtggtacg accagtccga tcgcacaatt tttcagccta gttgaggccg
      781 ccgaatgtgc cagtcaaaaa gtgcgctttt tttgtgcgct gtcatgcgtt actggtaaaa
      841 agtttcccgt cccgcaccag ttcacgcggg tagctgtttt tcagtcgcga accaatccgt
      901 ttggctaaac caatcggctg atgctggaaa gtaaccaaca cgtcatccgc cactggcgcg
      961 gcttgcgggt aaacatcgcg cccgcgatac cactcctccg cttcctgcgg tgtcagttca
     1021 aaagcgttta cattgtcggg ggaggcatgg gcaataaccg cttcatgctg ccagcgataa
     1081 cctttgttat gcgtttcggc aagtttaatc cctaagcgag aaaatcggac tttaccgatc
     1141 agggcttcaa tgcctaccgg gaacaaccac agttctttgt cgcgttgcca gagtcgcagg
     1201 tttccatccc agtttaagcc aacacttgca gccgcctgac gaatttgtcc ggcttcgcga
     1261 tctttcaccg ggctgaacgg gaaattgccc actttgtatt ttgggacggg taaggcgggg
     1321 atcgcctgag ttttacgcag acgagcaacg aagaagcctt cgcagtcgta aatttgtggg
     1381 aaaacatgca aaaaaccttc ttcggtcagc gccttgtttg caccagggaa gagatcgcca
     1441 agtggtaaaa actctacttc gtcggggtaa gtctctttca gccaccggca aacggcttcg
     1501 ttttcttccc ggtttaaggt acaggtcgag taaaccagcg taccgccagg acgtaatgca
     1561 tgaaaggcgc tgtcaatcag ttcccgttgg gtagctgcga tttcctgatt gctttcgggt
     1621 gaccagtttt ttagcgcatc gggatcttta cgcaccacgc cttcgccgga gcagggagcg
     1681 tccagcaaaa tggcatcaaa catttctggc actgacgcac caaacacgcg gccaccaaaa
     1741 tgtgtgagcg caacattact gatgccacag cggctgatat tggcatgtaa cacttttacc
     1801 cgactggcgg aaaactcatt ggcaaggatt gccccttcgt tattcatccg cgcggcaatt
     1861 tgcgtcgttt ttgagcctgg cgcagcagcg acatccatca cccgctgtgg tgcattaccg
     1921 tcagcaaaca aggcggcgac gggcaacatt gaactggctt cctgaatata aaacaggcca
     1981 cttaaatgct cggtggtact acccaatggc aatgcatctt cattgtcgcg ttcaatccag
     2041 aaaccttctt cacaccacgg aattggcgta agcgtccagc cataaggagc ggttaattgc
     2101 aggaaatcag caacggagat tttcagcgta ttaacgcgaa tgctgcggcg caacgggcgc
     2161 tgacaggcgg caagaaaatc atcaaatgag agcgtcgaag gcatagcttc gcgcatttgt
     2221 gtcagaaacg cgtccgggaa ataaacggcg tgttgggcca cgagcatgta ccactggcaa
     2281 aaaaacaggc gcgcagttta gcataaacgc tccggcgcag gcacgccgga gcagtgggca
     2341 ttatttggga agcgcagttc cccattcacg ccactctttc ggttcacttt cctgcaacag
     2401 gaagtgtttg ccttcctggg ctttcggtgc aagtggcgta cccggaggcg tggcgaaggc
     2461 gatgccgcca cggataaatt ggttaaaggt gccggttttc actacgccgc ccgtcagacc
     2521 aaagtccaga ctgtaacccg atgccaacca gaagacggaa ttgttacgca ccaggtgttg
     2581 atagcgttta ctgatgcgca tcgcaatcat cacgcgatct gacaatgtcc ccagcgtcat
     2641 tccggtaacc gtacctactt ccagaccacg gaacagcaca ggcgtaccga tgcctaacga
     2701 accggcttcc ggcgcttcaa caataatgct taaaccatct aagtaacgcg aatcagtaat
     2761 ggtggcctct tctaattcaa agtcgcgacg aggattgcca cggcctggtt cgacgttgat
     2821 atacggctgg aggatagtat ctagatgctc aacgccagct gccgagattt gcggtgtgac
     2881 cactgagaag cgcgtaccac cgcgagcaaa ggtctggaca tattccggat agagcaccgc
     2941 ctttgcctgt acttcattgc gtgcggtaat cagatccagc gtctggattt gcccgatatc
     3001 aataccgaga tagcgaattg gcatcccgac cgccagtttt ccggcatcga aagcgtgaag
     3061 cgtaatctgc ctgccaaccg cacgggccgc tgtttcggaa gcatacagaa ttcgcttgtc
     3121 gcctttacgc tgactggcgc tggcaccact gaggttatcg aagctaatgg ccccctttaa
     3181 tgctctggag agcggggatg cctgtacggt caggccacta ccattcagct gaactttcgc
     3241 cccgccttct gcctagaaca cgctgttgct ggtcaaaagg ttgcgatatt ccggtttaat
     3301 gtgcagatcg atatcgaacg cgttagctcg cggacgcacg gtaataactt caccaacttc
     3361 aaatttacgg tacagcacta ccgatcctgc ctgcacatcc ggcagcgtct ctgcactcaa
     3421 actcacggtt gtggtgggta aatcgctaag gctgttctcc agcgcttttt ccagattggc
     3481 atacagtgga tagctggctt tcatctcgcc tttatcgccc ggcagaatac gtatcccgcc
     3541 gttaatccat tctgaggcgc tggcaccgag aaactcaacg ccatccagcc ccaccttcac
     3601 gtcgacacgg ctgttgacga caaatttgct atcgcctttt accagttctc gatgctgagg
     3661 ctcgatggcg acggtaaagg tgacgccttt gctggtgagt ttacgatcga taacctggcc
     3721 tacctgcacg ccgtgaagaa tgagcggctg acccgcatca ataccgtaac tttccggtgc
     3781 tgtcagggtc agtgtcagaa cgtccggctc ttgcagcagg gctttttcgc ccggcacaac
     3841 aacgaactct ttgcgtggct cgccatcgcc gggcaccaac tcaaaggttt ttccggtcag
     3901 cagggcgctg agattagcat cgctaaggga taatttcggg ttgcgtaatt ctatgcgggt
     3961 attttcacga agcagggtaa cgacgctggg atcaacggtc atttccccag tgactttacc
     4021 accaggattt aaatccagtt tagtcagctg tccgacttcc agcccctgat acattaacgg
     4081 cgtcgagtcg gcggttaatc ctgctccact cggcagttcc agttttatta ttactccacg
     4141 ctggctgtgg gccagatctt catacagacc aaaggtatct tccgcctcgg caggtttcga
     4201 ctcttcaggt gaatcgaagg caatcgcacc gttaaccagt gccgccagac tttccagttt
     4261 caccttcgcg ccactgatac tgacgttggc atcaacgccg gaaacgttcc agaaacggct
     4321 accttttttc accagatcgg taaaacgccg ctcgatcagg acatcaatca ccacgccttg
     4381 cttgttggga ttgatggcat agtcgtagac ttttcccacc gggatcttgc ggaaatagac
     4441 caatgaaccg ctgttcagcg aaccgagatc gggggcttgc aggtggatca tcagatcgcc
     4501 attgtccagc cgatatttcg gttgggtatc gagcgcgaca aagtgatcct gctctttacc
     4561 tttacccggc atcatgccga tatagttccc accgacgagg gcgtccagcc cggagacacc
     4621 tgccaacgat gcttttggcg tcatcagcca gaactgagtc tcttcgcgca gcgcatcttt
     4681 catatcggac ttgatgctga ccttgacttc aatcttacga agatcgtcgc tgaggctgat
     4741 atcctgcact gttccgactt caacgccctg ataacgaaca ggcgtacggc ccggaacaat
     4801 accatccgcc gacataaagt cgatggtgac ggtattaccc cggtcctgat aactgtccca
     4861 aatcagccaa ccggcaatca ttagcgcgat gaaaggcagc agccagaaag gtgagatacg
     4921 gcgtttattt ttaatctgcg cttcagtcgt cgaagcgggc gtttcctgac tcatgtgcat
     4981 cccaaagtaa gcggctgtcc agccattcca cagcaagaat agtcaaaatt acggcagcgc
     5041 caaaataaaa cgcagccggt cccatagtaa aagcgaggat ctgatcgcga ttaatcagcg
     5101 acatggttaa agatatgaca aacaggtcga gcatcgacca gcgtccaatc caggtcacca
     5161 tccgcagtaa cagaatgcgt gtgcgtaaac cttgctggca tttaaaatga atgctcaaca
     5221 gtaaagtgaa catgacgatc actttagtaa acggtaccag aatactggcg ataaacacga
     5281 ttcccgcaac cgcaatgttg ctacttgcca gcgacataat tccggaaaga atcgtatctt
     5341 cctgccggcc accattcaga taaatgatag aaataggcaa taggttggct ggtaacaaca
     5401 aaacgattga cgctaacagc gccgcccagc atttttgcaa actatgacga cgacgcaggc
     5461 gtagcgggat atggcaacgc gggcagagac cacgctgatc aggatagccg gtaaaatggc
     5521 acccaagaca gacacgaagt ttctcgtccc tacgcgtagc ggggcgctgc ggataatatc
     5581 gctcccacag ctcctcaaca ttaagatgtg acaacgtcac cgtcgttaaa atcaccaacg
     5641 ccacaaaaga gaacaagccg acacccgcct ggatatgggc ataatcctgt accttatata
     5701 gaagcaacgc caatgccgac caggtagata tccagcatta cccactcttt aagtcgctca
     5761 agcatcagca gtaccggacg taaattcatt cccagtcggt taccaaacca taaatatgct
     5821 atggaggaca ccagaatgag gggggcacca ataacgcaga aaaagaccat tgatcccgtt
     5881 atcgcatcgc cctgtttggt catttgccag atgccttgca tgacgttggc gtcaatacga
     5941 atgcctaaca gccagatatg caacagcggt tcgccccagg caaacggcat aagcaacagc
     6001 atggtgaagg ccattgccgc caggcgcgtt agcgacaagt cgcgcccgtc gcgaattttt
     6061 gcctgacagc gcggacaata ggcactttga tgagaattta tctcgggcag gctaaacagc
     6121 atgtcacatt gcgggcaacg ttggtaatca ccacgcggca gttcctcgcc gattgccctc
     6181 actgttatct tttttgtcgg cgtaatttgt ggtgtgttaa gagccatgac gtgccaaatg
     6241 taataagaat taacattaat cttaacccat gatgggaatg atcttgagca ttaacgcatt
     6301 taatgcttat tttaataggc tggcagttcc aggtaagaca accaagtgat taaataatga
     6361 acaaaacaga attttacgcg gatttaaatc gcgactttaa cgcgctgatg gcgggagaaa
     6421 ccagttttct ggcaacgctt gcgaacacca gtgcgttgtt atacgagcgt ctcactgacg
     6481 ttaactgggc aggtttttat ttgcttgagg acgatacact ggtactcgga ccatttcagg
     6541 gcaaaattgc ctgtgtccgg atacccgtcg ggcgcggggt gtgcggcaca gcggttgccc
     6601 gcaatcaagt gcagcgtatc gaggatgttc acgcgtttga cgggcatatt gcctgtgatg
     6661 cggcgagtaa ttctgaaatt gttctgccgc tggtggtgaa aaatcagatt attggtgttc
     6721 tcgacatcga tagtaccgtc ttcgctcgct ttacagacga ggacgagcaa ggcttacgtc
     6781 agcttgtggc acagcttgaa aaagtgcttg caacgacgga ttacaaaaaa ttctttgcga
     6841 gcgtcgcagg ataatcaacg gataacgtag catttactgg tagcgtcatt ataatgacgc
     6901 ctgttcatgc ctgcgcttgt tggctacgtc cgttgtaatc aggaaatttc atggaaaatc
     6961 aacctaagtt gaatagcagt aaagaagtaa tcgcgtttct cgccgaacgt tttccccact
     7021 gtttcagtgc ggaaggtgaa gcgcgtccgc tgaaaattgg tatttttcag gatttggtcg
     7081 atcgtgttgc cggggaaatg aacctgagca aaacgcaatt acgatccgct ttacgtctct
     7141 acacttcgag ctggcgttat ctttacggtg ttaaacccgg cgcaacgcgt gtcgatcttg
     7201 acggcaaccc atgcggtgag ctggacgagc aacatgtaga gcatgctcgc aagcagcttg
     7261 aagaagcgaa agcgcgtgtt caggcacagc gtgctgaaca gcaagcgaaa aaacgcgaag
     7321 ctgccgcagc tgctggtgag aaagaagacg caccgcgccg cgaacgcaag ccacgtccga
     7381 ccacgccacg ccgcaaagaa ggcgctgaac gtaaacctcg tgcgcaaaag ccggtagaga
     7441 aagcgccaaa aacagtaaaa gcacctcgcg aagaacagca caccccggtt tctgacattt
     7501 cagctctgac tgtcggacaa gccctgaagg tgaaagcggg tcaaaacgcg atggatgcca
     7561 ccgtattaga aatcaccaaa gacggcgtcc gcgtccagct gaattcgggt atgtctttga
     7621 ttgtgcgcgc agaacacctg gtgttctgaa acggaggccg ggccaggcat gagcatgttt
     7681 tttaggctta ccgcgttagc tggcctgctt gcaatagcag gccagacctt cgctgtagaa
     7741 gatatcacgc gtgctgatca aattccggta ttaaaggaag agacgcagca tgcgacggta
     7801 agtgagcgcg taacgtcgcg cttcacccgt tctcattatc gccagttcga cctcgatcag
     7861 gcattttcgg ccaaaatctt tgaccgctac ctgaatctgc tcgattacag ccacaatgtg
     7921 ctgctggcaa gcgatgttga acagttcgcg aaaaagaaaa ccgagttagg cgatgaactg
     7981 cgttcaggca aactcgacgt tttctacgat ctctacaatc tggcgcaaaa gcgccgtttt
     8041 gaacgttacc agtacgcttt gtcggtactg gaaaagccga tggatttcac cggcaacgac
     8101 acttataacc ttgagcgcag caaagcgccc tggccgaaaa acgaggctga gttgaacgcg
     8161 cagtgggaca gtaaagtcaa attcgacgag ttaagcctga agctgacagg aaaaacggat
     8221 aaagaaattc gtgaaaccct gacacgccgc tacaaatttg ccattcgtcg tctggcgcaa
     8281 accaacagcg aagatgtttt ctcgctggca atgatggcgt ttgcgcgtga aatcgatccg
     8341 cataccaact atctttcccc gcgtaatacc gaacagttca acactgaaat gagtttgtca
     8401 ctggaaggta ttggcgcagt gctgcaaatg gatgatgact acaccgttat caattcgatg
     8461 gtggcaggtg gtccggcagc gaagagtaaa gctatcagcg ttggtgacaa aattgtcggt
     8521 gttggtcaaa caggcaagcc gatggttgac gtgattggct ggcgtcttga tgatgtggtt
     8581 gccttaatta aagggccgaa gggcagtaaa gttcgtctgg aaattttacc tgctggtaaa
     8641 gggaccaaga cccgtactgt aacgttgacc cgtgaacgta ttcgtctcga agaccgcgcg
     8701 gttaaaatgt cggtgaagac cgtcggtaaa gagaaagtcg gcgtgctgga tattccgggc
     8761 ttctatgtgg gtttgacaga cgatgtcaaa gttcaactgc agaaactgga aaaacagaat
     8821 gtcagcagtg tgatcatcga cctgcgtagc aatggcggtg gggcgttgac cgaagcggta
     8881 tcgctctcag gtctgtttat tcctgcgggt cccattgttc aggtccgcga taacaacggc
     8941 aaggttcgtg aagacagcga taccgacggg caggtgttct ataaaggccc actggtggta
     9001 ctggttgacc gtttcagtgc ttcggcttca gaaatctttg ccgcggcaat gcaggattac
     9061 ggtcgtgcgc tggttgtggg tgaaccgacg tttggtaaag gcaccgttca gcaataccgt
     9121 tcattgaacc gtatttacga tcagatgtta cgtcctgaat ggccagcgct gggttctgtg
     9181 cagtacacga tccagaaatt ctatcgcgtt aacggcggca gtacgcaacg taaaggcgta
     9241 acaccagaca tcatcatgcc gacgggtaat gaagaaacgg aaacgggtga gaaattcgaa
     9301 gataacgcgc tgccgtggga tagcatcgat gccgcgactt atgtgaaatc aggagattta
     9361 actgcctttg aaccggagct gctgaaggaa cataatgcgc gtatcgcgaa agatcctgag
     9421 ttccagaaca tcatgaagga tatcgcgcgc ttcaacgcta tgaaggacaa gcgcaatatc
     9481 gtttctctga actacgctgt gcgtgagaaa gagaataatg aagatgatgc gacgcgtctg
     9541 gcgcgtttga acgaacgctt taaacgcgaa ggtaaaccgg agttgaagaa gctggatgat
     9601 ctaccgaaag attaccagga gccggatcct tatctggatg agacggtgaa tatcgcactc
     9661 gatctggcga agcttgaaaa agccagaccc gcggaacaac ccgctcccgt caagtaatat
     9721 caatcaggca caagaaattg tgcctgattt tttaacagcg gcaagatgcc gtaaatcaga
     9781 tgctacaaaa tgtaaagttg tgtctttctg gtgacttacg cactatccag acttgaaaat
     9841 agtcgcgtaa cccatacgat gtgggtatcg catattgcgt tttgttaaac tgaggtaaaa
     9901 agaaaattat gatgcgaatc gcgctcttcc tgctaacgaa cctggccgta atggtcgttt
     9961 tcgggctggt actgagcctg acagggatac agtcgagcag cgttcagggg ctgatgatca
    10021 tggccttgct gttcggtttt ggtggttcct tcgtttcgct tctgatgtcc aaatggatgg
    10081 cattacgatc tgttggcggg gaagtgatcg agcaaccgcg taacgaaagg gaacgttggc
    10141 tggtcaatac tgtagcaacc caggctcgtc aggcggggat cgctatgccg caagtggcta
    10201 tctaccatgc gccggacatc aacgcttttg caaccggtgc gcgccgtgat gcctctctgg
    10261 ttgctgtcag caccggtttg ctgcagaaca tgagcccgga tgaagccgag gcggtaattg
    10321 ctcacgaaat cagccacatc gccaatggtg atatggtcac catgacgctg attcagggcg
    10381 tggtgaacac cttcgttatc tttatttccc gtattctggc gcagcttgcc gcgggtttta
    10441 tgggcggaaa tcgtgatgaa ggtgaagaga gcaacggcaa cccgctgatc tactttgcgg
    10501 ttgcaacggt tctggaactg gtgtttggta ttctggcgag cattatcacc atgtggttct
    10561 cgcgtcatcg tgaattccat gctgatgccg gttcggcaaa actggttggt cgcgagaaaa
    10621 tgattgccgc gctgcagcgc ctgaaaacca gctatgaacc gcaagaagca accagcatga
    10681 tggctttctg cattaacggt aagtcgaaat cgctcagtga gttgttcatg actcacccgc
    10741 cgctggataa acgtattgaa gctctgcgta cgggtgaata cctgaagtaa tatgaagtaa
    10801 atactaaaaa gcgcgtcctg atcgacgcgc tttttttatg ccctggctcg tggctgagtg
    10861 atacgtaaac cactgacaca ggcagcaatc actgccagaa tagccgcagc catcagcgag
    10921 acgtgcgtac cattatcacc aaactgattt agcatcagcg ccaccagcgc cgcgccacta
    10981 ctctgaccca gtagacgagc cgttcctaac atgccactgg ccccaccgct acgttcgcgc
    11041 ggagcggagg taataatggt gtggttattg ggtgactgga ataagccaaa tccagcgcca
    11101 cataagatca tcggccagat aatattgata tccgcaggtg acgcgggcag cagaaccagg
    11161 gaaaaaagcc ccgcagccat gatgaacaac cctaaagcac ccagcaatcc tgcatgtacg
    11221 cgttcaatca aatagcctgc cagcggagcc atcaccatcg ttgctaacgg ccacggtgtc
    11281 agaagtaaac ctgtttcgac ttcactacgc ccgagcacgg tttgcaggta aaagggcagg
    11341 gaaaccattg ccagcatttg tgcgcagaaa gagcaaacag atgtgcaaat agaaagtgaa
    11401 aacagcggga tacgcagtaa atccaccggt agcagcggta cgggaagaga aagctggcgg
    11461 cgaataaaga aaataccaac aacaaccatt accaccagtt ccgcagcaat taacgtcagc
    11521 gattgcccct gagcgaaacc actcaacgca gtgataagca ggccgaaggt taacgcgttc
    11581 atcacggcgc tgggcaggtc gaaacggggt ttactggcgc gagaaccatt gggtggcaga
    11641 aaacgcatcg ccagaagcag ggcgataata cctaacggta cgttgattaa aaataaccat
    11701 ttccaggatg cgatggagag gattgctgca gcaattgtcg gcccggcagc agaagagacg
    11761 gcaacaataa acgagtttat gcccatccct ctccccagaa aacgttgtgg atagatcagg
    11821 cggataagtg cggtattaac gctcatcaac gctgcaccgc cgaaaccttg tatgacacgc
    11881 gcaagggtga gcatttgcag cgaatcagaa agggcgcaga acagtgaaga caacagaaaa
    11941 acgaccagac cgcatttata aatacgtcga tagccaaaca tatcgcccag aaacgaaaac
    12001 gagagcaggg agatgacaat ggcgatttga taggcgttca ctacccagat ggaactggct
    12061 ggtgtggcat gaaggtccgt ggcgattgtt ggcagggcga cgttggcgat tgcgccgtca
    12121 aggacagcca tcgaaatacc aatcacaatg gttaatatcg caccgtatcg ctggggcaat
    12181 ggcaggccgt cggcctgaac ttttggcata ggaattttat atctttggtg aataatcaac
    12241 aggggtatga ttctagcact gatattcacg gtggatgtcg cagaattgta actaaccggc
    12301 aaaagatgga ttgcgaccac cctggtgcga aattataata aaaaacagtt ctgattttta
    12361 taaaacactc gcaatgaggt gataaatggc taacgcagat ctggataaac agcctgattc
    12421 tgtatcttcc gtgctaaaag tttttggcat tttgcaggcg ctgggtgaag agcgcgaaat
    12481 agggataacc gagctgtcgc agcgcgtcat gatgtcaaaa agcaccgttt atcgcttttt
    12541 acagaccatg aaaaccttag gttatgtggc gcaggaaggg gagtcggaga aatattccct
    12601 gaccctgaaa ttgtttgaac tgggcgctcg cgcgttacaa aacgtcgatt taattcgtag
    12661 cgcagatatc cagatgcgtg agatctcccg cctgaccaaa gaaactatcc acctcggcgc
    12721 actggacgaa gacagtattg tttacattca caaaattgac tctatgtaca atttgcgcat
    12781 gtattcacgg attgggcgtc gtaatccgct gtacagcacc gcgattggta aggtactgct
    12841 ggcatggcgc gatcgcgatg aagtgaagca aattcttgag ggcgtggagt ataaacgcag
    12901 taccgagcgg accatcacca gtacagaagc gttattaccc gttctggacc aggtgcgcga
    12961 gcaggggtat ggcgaagata acgaagagca ggaagaaggg ctgcgatgca ttgcggtacc
    13021 ggtatttgat cgctttggcg tggtcattgc cggtttgagc atctccttcc cgacgttgcg
    13081 tttctctgaa gagcgtttac aggaatatgt cgcaatgttg catactgcag cgcgcaaaat
    13141 ttctgcccaa atgggttatc acgactatcc gttctgatga gagtaagaac ctgtcggaat
    13201 atcaaacaga caggttcttt atttagcatg agaaaaataa agttgaaggt gcgttatatt
    13261 aaacgcgctt gctataagaa tattttactc atgagtgaga atctggttat ttattaccct
    13321 taaccattat cgaccacgat attgctttta cgtaacagtg ggcaatctgt tatccccaag
    13381 aaaccacttt tagtgtgcaa gtattgtacc gtgctggtgc ctctggcagt cagataggta
    13441 cattgcaaac ctaatcctgc ggcattctct ttgcttccaa tcaaaacgcc atatccgctg
    13501 agtaataatc ctatccatac cagtgctatc agcataactg tgcgaatgat gaatcgcatt
    13561 acaatctctt ctctttttat gttcgcttaa tcgtagcggc aatatgcgct gaagcaagct
    13621 actcattccg aaaaagcacg aatatcgaca tagttaggcg ctgtttaact aacgcatgct
    13681 agtttaatga cataaggtag gtgaagcgga gattggagtg aaaaagtttc gatgggtcgt
    13741 tctggttgtc gtggtgttgg cttgcttgct gctttgggcg caggtattca acatgatgtg
    13801 cgatcaggat gtacaatttt tcagcggaat ttgtgccatt aaccagttta tcccgtggtg
    13861 atatcatttt ttcagcacta gattttcttc ctctccagca gtggtagaat agcgcctcta
    13921 ctcagaggtg gtgaaatgaa cgaagttgta aattcaggcg tgatgaacat tgcgtctttg
    13981 gttgtatcgg tggtggttct tcttatcggg ctcatcttgt ggttttttat caatcgtgcc
    14041 agttcacgga ctaacgaaca gattgaactg cttgaggcgt tgctggatca gcaaaaacgt
    14101 caaaatgcac tgttacgtcg tttgtgcgaa gcaaacgaac cagagaaagc agataaaaag
    14161 accattgaga gtcaaaaatc ggttgatgac gaagatatta ttcgcctggt cgccgaacga
    14221 taatccttcc tcaatcttga gtaaccccgt cgttgaaggg ttactcaatt catcctgatt
    14281 atacgcactg tcatggtgca actgcttcaa attaaaactc tggtctgact tcaaataatt
    14341 atcatattat ttatttgcgt aacgggatat cagaaaaaga tattttcctc attaatttat
    14401 taggatgttt acatcagatt tgtgattaag cgtggtatta tttattagcg aaacgtttct
    14461 ctcttgattt ttttgcgcac tcatcaattt ttcttatttt aaatttacaa tccctttggg
    14521 gattgacttc tctttagggt aattgatagc cgttaactga ctgttttatg agaaaaagtg
    14581 atataacttt ttattcattg catagcagaa aatgtgatat tgcacgcact atgtaataac
    14641 ttctccctct ggcctggaac aactgaactt attgaactat gttagaaact acgccagttt
    14701 aagtatctgc ctgaactggc aaggttaagc acaatgatat atcggcgcgt attccgttgc
    14761 ataagtgtgc aaaaaaagtg gaagacgtat cgagatttgt gcgtctgatc gagacatgtt
    14821 taaaaatggc ttgccataat taacgttgta tgtgataaaa gatttcgggt taaacgaggt
    14881 acagttctgt ttatgtgtgg cattttcagt aaagaagtcc tgggtaaaca cgttgacgtt
    14941 gaataccgct tctctgccga gccttatatt ggtgcctcat gcagtaatgt gtcagtttta
    15001 tctatgttat gcctgcgggc gaagaaaaca atctaaggaa tttttcaaat ggcaaagatt
    15061 aaaggtcagg ttaagtggtt caacgagtct aaaggttttg gcttcattac tccggctgat
    15121 ggcagcaaag atgtgttcgt acacttctcc gctatccagg gtaatggctt caaaactctg
    15181 gctgaaggtc agaacgttga gttcgaaatt caggacggcc agaaaggtcc ggcagctgtt
    15241 aacgtaacag ctatctgatc gaatccactg atctgaagtg tgaatacgct tcaatctcgc
    15301 tataaagcct cgtcgaatgc gaggcttttt actatgcttt atcttcgctc ctggcgttcg
    15361 gatatttgcc agccacgtga ttcgcgttac acttgcggcc tttagtatcc tgccggagtt
    15421 gtcatgtcgt tttcctgtcc actttgccat cagcctcttt cgcgtgaaaa aaacagctat
    15481 atctgtcccc aacgacatca gtttgatatg gcgaaagaag ggtatgtcaa tctgctgccc
    15541 gttcagcata aacggtctcg tgatccgggc gacagcgcgg aaatgatgca agcacgccgc
    15601 gcattcttag atgccggaca ttatcagccg ctgcgtgatg caattgtcgc ccaactgcgg
    15661 gaacggcttg atgaaaaggc cacggcggtg ctggatattg gctgtggtga agggtattac
    15721 acacacgcat ttgccgatgc gttgcccgaa atcaccacgt ttggcctgga tgtctcgaag
    15781 gtagcgataa aagcggcggc gaaacgctat ccgcaggtca ctctttgtgt cgcttccagc
    15841 caccgtttgc cgttttccga taccagtatg gacgccataa tacgtattta cgcgccgtgt
    15901 aaagcagaag aattagcacg agtagtgaag cccggcggct gggtcattac tgccacgccg
    15961 ggaccgcgac atttgatgga gctgaagggg ctgatttaca atgaagtaca tcttcatgca
    16021 cctcatgcag aacaactgga aggttttaca ttacagcaga gtgcggagtt gtgttatccg
    16081 atgcgtcttc gcggtgatga agccgtcgca ttattgcaga tgacgccgtt tgcctggcgt
    16141 gcgaagccag aagtctggca aacactggca gcaaaagaag tgttcgactg ccagacggac
    16201 tttaatattc acctctggca gcgttcttat taaccgtgga agtgcgtcca gaggatctgg
    16261 acgccgatgc cgatcagcac cagcccgccg agaatttccg cttttttccc aataattgag
    16321 ccgataaagc gaccaaccat catccctaat gtcgacataa tcaaggttgc acaaccaatg
    16381 gccaatgcgg tcgcgataat gttgacctga aggaaagcaa gaccaacacc cacagccatg
    16441 gcatccaggc tggtggcaat cgcggtggtt accagtagcc agaaaccgtg ccgacggcgc
    16501 ggctcttcat cttcatcatc tgagccacga aaaccctcaa taatcattcg cccgccgagg
    16561 aatatcagca gcacaaacgc aatccagtga ttccattcaa ggacaaaccg gctggctaac
    16621 atgcccattc cccagccgat cagcggcgtc agggtttcga cggcaccaaa aataaggccg
    16681 gttcgcaatg cttcagaaaa tttcggttta tggagggggg cacctttacc gattgatgca
    16741 gcaaatgcat ccatcgacat accaaacgca agaagaacag tagcagtgat attcatgaca
    16801 acgtcctgac cggggtatcc ataaaacaca tcatcgcccc cagcaaacat gcatacatga
    16861 acaacaagcc gcgatgggtt ttcgcgttca tgcaggcata gcgattgatg tgcatatggt
    16921 ctcgcctgac tgaaagtctt cagtccgtac gtgccacgtt ttgcaacgag tatgttgaca
    16981 cgtacatttc cggaatctgg aaatcggcta ctccccaaat gagggcgcaa ccttaacata
    17041 ttttgagaat ataaaacaac tgttggaata tattatttca ttgctttatt gataacgatt
    17101 ttcatttatg tttatatatc aatgaaacgt taaaataaat aacaaataag ggaagagata
    17161 atatagccta agctatgtct gattattaaa aatacactaa acatagcttt ggctaaattc
    17221 ataagttatt gagttgata
//
LOCUS       DAJTYG010000078        17099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTYG010000078 DAJTYG010000000
VERSION     DAJTYG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.078"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(464..1660)
                     /gene="hemY"
                     /locus_tag="OO568_002837"
     CDS             complement(464..1660)
                     /gene="hemY"
                     /locus_tag="OO568_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6642122.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1663..2874)
                     /gene="hemX"
                     /locus_tag="OO568_002838"
     CDS             complement(1663..2874)
                     /gene="hemX"
                     /locus_tag="OO568_002838"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6642123.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(2896..3636)
                     /gene="hemD"
                     /locus_tag="OO568_002839"
     CDS             complement(2896..3636)
                     /gene="hemD"
                     /locus_tag="OO568_002839"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6642124.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFTIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3633..4574)
                     /gene="hemC"
                     /locus_tag="OO568_002840"
     CDS             complement(3633..4574)
                     /gene="hemC"
                     /locus_tag="OO568_002840"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6642125.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4961..7507
                     /gene="cyaA"
                     /locus_tag="OO568_002841"
     CDS             4961..7507
                     /gene="cyaA"
                     /locus_tag="OO568_002841"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6642126.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7547..7867)
                     /gene="cyaY"
                     /locus_tag="OO568_002842"
     CDS             complement(7547..7867)
                     /gene="cyaY"
                     /locus_tag="OO568_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6642127.1"
                     /translation="MNDSEFHHLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7915..9228)
                     /locus_tag="OO568_002843"
     CDS             complement(7915..9228)
                     /locus_tag="OO568_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6642128.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9225..10703)
                     /locus_tag="OO568_002844"
                     /pseudo
     CDS             complement(9225..10703)
                     /locus_tag="OO568_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            10892..11095
                     /locus_tag="OO568_002845"
     CDS             10892..11095
                     /locus_tag="OO568_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6642129.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11132..11956
                     /gene="dapF"
                     /locus_tag="OO568_002846"
     CDS             11132..11956
                     /gene="dapF"
                     /locus_tag="OO568_002846"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6642130.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            11953..12660
                     /locus_tag="OO568_002847"
     CDS             11953..12660
                     /locus_tag="OO568_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6642131.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12657..13553
                     /gene="xerC"
                     /locus_tag="OO568_002848"
     CDS             12657..13553
                     /gene="xerC"
                     /locus_tag="OO568_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6642132.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13553..14269
                     /gene="yigB"
                     /locus_tag="OO568_002849"
     CDS             13553..14269
                     /gene="yigB"
                     /locus_tag="OO568_002849"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6642133.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14353..16515
                     /gene="uvrD"
                     /locus_tag="OO568_002850"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14353..16515
                     /gene="uvrD"
                     /locus_tag="OO568_002850"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6642134.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACSELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16662..17054)
                     /locus_tag="OO568_002851"
     CDS             complement(16662..17054)
                     /locus_tag="OO568_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6642135.1"
                     /translation="MRPGGVFYVAGDKVGIVRLDAFKASKDIQFAVQSGPMLMENGVI
                     NPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVEQLLYLGGT
                     ISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         3958 a         4428 c         4542 g         4171 t
ORIGIN      
        1 ttgatgctgt ttttagtttt aacggcaatt aatatatgtg ttattaattg aatgaatttt
       61 atcattcata ataagtatgt gtaggatcaa gctcaggtta aatattcact caggaagtta
      121 ttactcagga agcaaagagg attacagaat tatctcataa caagtgttaa gggatgttat
      181 ttcccgattc tctgtggcat aataaacgag tagatgctca ttccatctct tatgttcgcc
      241 ttagtgcctc ataaactccg gaatgacgca gagccgttta cggtgcttat cgtccactga
      301 cagatgtcgc ttatgcctca tcagacacca tggacacaac gttgagtgaa gcacccactt
      361 gttgtcatac agacctgttt taacgcctgc tccgtaataa gagcaggcgt ttttttatgc
      421 atcagaaagg tcccggaggt gctttcctcc gggtgagaag gaactactgt ggcgggttat
      481 tctgcaacgt taacatcaaa ccgtcgcgac gcatcgctgc agcttcttcc ggcttgtgca
      541 gtctgtccag cgcgtcggca agccatgcgt aatcgtaggc gtccggacgt tgtttcagcg
      601 ccgcgcggaa ggcgagcgat gcttcctgcc attcaccgtg cttcatcagt gactgaccca
      661 atgtgctcca caacagcggg cgatcaccga cgtttttgat ttgctggcgt agcacttttt
      721 ccagctgttc cgggttattg gttttcagac gcgggatcgg cagcagtagg cgatcgtcat
      781 actggcgttt caggccgtcg atgataattt gctgggcagt atcatgatcg tcacattcaa
      841 taagatgttc cgccattgcc acctgcaacg ctacctgatg acgcgttttc cggctttggt
      901 ttttccacca gttacgcaaa ccttcactac cgttatcggc acgcgcctga tccatcaggc
      961 caatccatgc ctgttgttcc agcattgcac gatgttcttc atcaccaaca tgggctttcg
     1021 ccattgatgg gatgatatcc agcagagaac tccatgcccc tgtgcggata tacgcctgtt
     1081 ccgccagacg taatacttca ggatggcgtg gcgtaacttc cagcagctta tccacgccgt
     1141 ggcgtgcagc atggttttca ttacgggcca gttgcagacg tacacgggtg atttctaccg
     1201 gaatggtgtc attgccggcc agctccgctg cgcgttccag atgttggttg gcgcgtgctt
     1261 catcaccacg ttgttgcgcg gcttcggcag ccagtagata gttcaccacc ggttgttcag
     1321 cgtgatcggc atttttcgcc atcagctttt caacttgctg ataatcacct tccgccagtt
     1381 tcagcagcgc ctgttcggtc tgcttacgcg cacggcgacg cttacgtccg acaaaccatc
     1441 cacgggtgtg cgcgccagtg cggaagatcc gccgcagtag ccactcaatg gcaaacagca
     1501 ctaccatcgc cagaatcaat atgatcgcca ggcccgtgac gctggtttcg atattgtagt
     1561 tgtcggtctg gatcagcaca taaccctgat gaccggcaat catcgggcca accactatcc
     1621 ccgcaatcag caaaacaaag agtaataaca cttttagcat ggttattctc cttgcggcgc
     1681 ggctgccgga gtatcacctt gcggtgcagg tgcaggtgca ggtgcaggtg caggtgcagg
     1741 tgcaggttta gcttccgttg tccccgctgc cggttgtgcc agcaggttac gcacgcgagt
     1801 ctgcatcagt ttttccagca tcgcctggct ttgcagggtt tccggaagat ccatcgagat
     1861 attttgctgg cttaactggt ccacctcgtc gaggaacgct ttggtggtgg catcatcagt
     1921 atcgtagtaa gcacgtaccc aggtggagac gttctccagc gcctggcgat aagtctcttc
     1981 ctgatgacgc ggtacagctt gtgctgcgac cagcaggcga gagcgaatat tttcgcgcag
     2041 atagatatcc tgatttggcg ctaacagcgg tacggcggtg tcatcacgac ggcgaatcgt
     2101 aatgaagttg tccataaagt tctgccagct tttttgcaga ttgatacgcc attcgctgat
     2161 ggaactggaa agctcttcac cgtctgaatc catcggcgaa ccatcgctgt cattatcggc
     2221 cagacgcagg ttatctacct gatttgaaag ctgattaagc ttaaggatga tgccgtcata
     2281 atccacctgc gatactgcag aaaggctggc gatatcatcg gtaattgccc gacgaacggt
     2341 aatcagactc gggtcattca tatccgccag gctggcgtct gcacttttca gcaacgctgc
     2401 agaggtcgtg acgtcctgat cgctccacag cttccgtccg gcgagtttca ccagaaaatc
     2461 ggcctgagcc agcagccagg ttttagcatc gctgccggaa atggtggcga tcttttgttg
     2521 gacttcatcc aactgttttg ccagcgtttc ttgctgacga ttcgcctgct taagctgcgc
     2581 agcttgttgc ttaataatgc cttccagctc ggctttttgg ctctcctggg ctttttgcaa
     2641 tgccgtcagt tggttagcca gggcatcgct ggtggcggtc tgattgacgg cctgttgttt
     2701 accccagcca tacaaaccga cacccgccgc cagagcaata gcgatagcca ccgcgctgag
     2761 aatcaatgca gtattgttct tactcttttt ttctgttgcg acaggttgtg acgtggtgtc
     2821 cacggcctcc ctggtctctt caaccacggc ggaggttttt tcttgttccg tcattatggc
     2881 ttcctgttat gagagttatt gtaatgcccg taaaagcgca tcgttgtcag cgttatcggc
     2941 gaccttaatg tcttgccagc ccagttcccg ggcgagtttc gccaaacgct cactgacgac
     3001 caatagtcga cagtgtagta accagtgctc acgataccat tgtgggatca gcgaccagag
     3061 ttgctgcaac atttcaccgc tggtaacaac gaccatcgtc acctcgcggg attgccagcg
     3121 catcgcttct tctgcaccat cgtaatggat tgcgcatcgt tgataacatt cacaaaaagt
     3181 gacctcagca ccgcgcaccg tcagggtatc cccaattagc tcacgaccgc cattgccacg
     3241 taatatcagc gcacgtttgc ccgcaatatt ttgtaattca ggtaattgta gcaagacttc
     3301 gctgatttcc cgatcctgtg ggtagagaat cttctgtccg cttacggtat gtagcgccag
     3361 tgcggtggtg cgtccaatgg tgaaataagt aggtagttgg ggccatttac gatcttgctg
     3421 atgcagctgt gattgggcaa aagcaaccgc gtgttgcgag agggcaaaca acagatcgct
     3481 ctcccccagc gctgccagtt gatcagcaag ttgcggtaat tgtcgacccg gagaaaactc
     3541 aatcagcgga aaatgccagg ccacctgccc cagtgtgcgc agacggctca ctaactcttc
     3601 tccagcggga gacgggcggg tgaccaggat actcatgccg gggcctctcc gttatagact
     3661 tcagcgagga tctcgcgcgc gccgttattc agtagctctt ctgccagcga aatccccatt
     3721 tgttcggcat cttgcggcgc accgcggcgt tcaccgcgaa taatctgcga accgtccggc
     3781 gcgccgacca gcgcacatag ccagatttcg ccatcaataa gctcggcgta gctaccaatt
     3841 ggcacctgac atccgccttc gagacgggta ttcatggcgc gttctgcggt aacgcgcagt
     3901 gcagtttcgt ggtgattcag cgcggcaagc agctcgcgag tgcgtgtatc atcaaggcgg
     3961 cattcaatac cgactgcacc ttgtcctacc gccggaagag aaatctcggg tggtaacgcg
     4021 gcgcgaatgc gggactccag acctaaacgt tttagtccgg ctacggcaag aatgatggca
     4081 tcgtattcgc cgttatctag tttgctcagg cgagtgccga cgttgccgcg cagggagcgg
     4141 ataatcagat ccgggcggcg ttcagccagt tggcactggc gacgtaaact ggacgtcccg
     4201 acgatactgc ctgctggtaa cgcatccaga ctgtcatagt tattggacac aaaggcatcg
     4261 cgaggatctt cacgctcgca aatagtgacc agccccagac cttgcgggaa ttcaaccggc
     4321 acatctttca ttgaatgtac ggcgatatca gcgcgattct ccaggagcgc gacttccagc
     4381 tcttttacaa ataagccttt tccgcctact ttcgccagcg gcgtatcaag aatcacatcg
     4441 ccgcgcgtca ccatcggtac cagttcaacg accaggcccg gatggctcgc catcaacttg
     4501 tctttgacat agtgtgcctg ccagagtgca agtgggcttt ggcgtgtggc aattcttaaa
     4561 acattgtcta acatgcttgt taccgtcatt atcatccgtg gtccatccta acatccttgc
     4621 cagagtgatg tcagtgttgt ggtgaaacgt agacgcctgc gcaaaccgta aaatgaggtc
     4681 tggcagtgga tcctgacagg cgtttcacgc cgtcgtaata aggaatttac agagaataaa
     4741 cggtgctaca cttgtatgta gcgcatcttt ctttacggtc aatcagcaag gtgttaaatt
     4801 gatcacgttt tagaccattt tttcgtcgtg aaactaaaaa aaccaggcgc gaaaagtggt
     4861 aacggttacc tttgacatac gaaatatccc gaatgccgcg tgttaccgtt gatgttggcg
     4921 gaatcacagt catgacgggt agcaaatcag gcgatacgtc ttgtacctct atattgagac
     4981 tctgaaacag agactggatg ccataaatca attgcgtgtg gatcgcgcgc ttgctgctat
     5041 ggggcctgca ttccaacagg tctacagtct actgccgaca ttgttgcact atcaccatcc
     5101 gctaatgccg ggttaccttg atggtaacgt tcccaaaggc atttgccttt acacgcctga
     5161 tgaaactcaa cgccactacc tgaacgagct tgaactgtat cgtggaatgt cagtacagga
     5221 tccaccgaaa ggtgagcttc caattactgg tgtatacacc atgggcagca cctcgtccgt
     5281 agggcaaagt tgttcctctg acctggatat ctgggtctgt catcaatcct ggctcgatag
     5341 cgaagagcgc cagttactac aacgtaaatg cagcctgctg gaaagctggg ccgcctcgct
     5401 gggtgtggaa gtcagcttct tcctgattga tgaaaaccgc ttccgtcata atgaaagcgg
     5461 cagcctgggg ggcgaagatt gtggctccac ccagcatata ctgctgcttg acgaatttta
     5521 tcgtactgcc gtgcgtctcg ccggtaagcg tattctgtgg aatatggtgc cgtgcgacga
     5581 agaagagcat tacgacgact atgtgatgac gctttacgcg cagggcgtgc tgacgccaaa
     5641 tgaatggctg gatctcggtg gcttaagctc gctttctgct gaagagtact ttggtgccag
     5701 cctttggcag ctctacaaga gtatcgattc cccatacaaa gcggtgctga aaacactgct
     5761 gctggaagcc tattcctggg aatacccgaa cccacgtctg ctggcgaaag atatcaaaca
     5821 gcgtttgcac gacggcgaga ttgtatcgtt tggcctcgat ccatactgca tgatgctgga
     5881 gcgtgttact gaatacctga cggcgattga agatttcacc cgtctggatt tagtacgtcg
     5941 ctgcttctat ttaaaagtgt gcgaaaaact cagccgtgaa cgcgcctgtg taggctggcg
     6001 tcgcgcagtg ttgagccagt tagtgagcga gtggggttgg gacgaagctc gtctggcaat
     6061 gctcgataac cgtgctaact ggaagattga tcaggtgcgt gaggcgcaca acgagctgct
     6121 cgacgcgatg atgcagagct accgtaatct gatccgcttt gcgcgtcgca ataaccttag
     6181 cgtctccgcc agtccgcagg atatcggcgt actgacgcgt aaactgtatg ccgcgtttga
     6241 agcattgcca ggtaaagtga cgctggtaaa cccgcagatt tcacccgatc tctcggaacc
     6301 gaatctgacc tttatttatg tgccgccggg ccgggctaac cgttcaggtt ggtatctgta
     6361 taaccgcgcg ccaaatattg agtcgatcat cagccatcag ccgctggaat ataaccgtta
     6421 cctgaataaa ctggtggcgt gggcatggtt taacggcctg ctgacctcgc gcacccgttt
     6481 gtatattaaa ggtaacggca ttgtcgattt gcctaagttg caggagatgg tcgccgacgt
     6541 gtcgcaccat ttcccgctgc gcttacctgc accgacaccg aaggcgctct acagcccgtg
     6601 tgagattcgc catctggcga ttatcgttaa cctggaatat gacccgacag ctgcgttccg
     6661 caatcaggtg gtgcattttg atttccgtaa gctggatgtc ttcagctttg gcgagaatca
     6721 aaattgcctg gtaggtagcg ttgacctgct gtaccgcaac tcgtggaacg aagtgcgtac
     6781 gctgcacttc aacggcgagc aatcgatgat cgaagccctg aaaactattc tcggcaaaat
     6841 gcatcaggac gccgcaccgc cagatagcgt ggaagtcttc tgttatagcc agcatctgcg
     6901 cggcttaatc cgtactcgcg tgcagcaact ggtttctgag tgtattgaac tgcgcctttc
     6961 cagcacccgc caggaaaccg ggcgtttcaa ggcgctgcgc gtttctggtc aaacctgggg
     7021 cttgttcttc gaacgcctga atgtatcggt acagaagctg gagaacgcca tcgagtttta
     7081 tggcgctatt tcgcataaca aactgcacgg cttgtcagtg caggttgaaa ccaatcacgt
     7141 caaattaccg gcggtggtgg acggctttgc cagcgaaggg atcatccagt tctttttcga
     7201 agaaacgcaa gacgagaatg gctttaatat ctacattctc gacgaaagca accgggttga
     7261 ggtgtatcac cactgcgaag gcagcaaaga ggagctggta cgtgacgtca gtcgcttcta
     7321 ctcgtcatcg cacgaccgct tcacctacgg ctcaagcttc atcaacttca acctgccgca
     7381 gttctatcag attgtgaagg ttgatggtcg cgaacaggtg attccgttcc gaacaaaatc
     7441 gattggcaac ctgccgcccg ccaatcagga tcacgatacg ccgctattac agcagtattt
     7501 ttcgtgatga gcgtgccgga aagcgaggct tatccggcat gcaatcttag cggaaactga
     7561 ctgtttcacc cgcctgttgc gtcgccgcct gttccagcaa atcccagaag gtttcgccgc
     7621 tgcgatcgca aatccattca tcgcctttca ggtcaaaatg gtagccgccc tgtttggttg
     7681 ccagccacac ctggtgcagc ggctcctggc ggttaataat gattttgctg ccgttttcaa
     7741 aggtaatggt cagtacgccg ccgttgatct cgcagtcgat atcgctgtcg ccatccctgt
     7801 cgtccagacg ctcttcaatg gtcagccaca gttgatcagc caggtgatga aattcactgt
     7861 cgttcattgt tgtaccctgt ttttaagtga tggcggcagt atagcggcat ggggtcaggg
     7921 cttcaaagtt tgcacctctg ctgctgcgtt ccggcatgat tcatccgtca ccggaataat
     7981 gatgtctctg ttttgaactt cacgtacgtt ctcctctctg acacgagttt tcgccgggca
     8041 gggggagggc gtaatcctta cctctgtatc ggtccatgta taaacattgc gatcgtcata
     8101 agcgaagcga tcgtttttag ttagcggacc gttaactgga ataatttgca agctctttga
     8161 ccgtaattgg cggataaagt aagcatgctg tttgccgcta ttaaatgagc gatcaatgac
     8221 caagtctggc gtatccagcg ggattatctc aagctggtct tcaccaaatg tcgataattt
     8281 tgttacatac agccgatctt tatattgcgc aatattgcca gtaatcagat ggaattgttc
     8341 aggttcaata cctggtaggt ctatccactt gctccactga cggtcgggcg tgagctgttt
     8401 acgccagcga acgcgacttt cccctacttc aaaagagacg tcatcatcta cgggtatcgc
     8461 tttgcctgat aattgaccaa agggataacg atgcagaccc ttattgtcgc gaaaaaccag
     8521 cagtgaatga ggtatccagc gaattatctc aaatgagtct gcattcgccg taaggtgaac
     8581 gccatttaaa taaatattct gcccactgcg gataagcaga tcattggaat atgaatttct
     8641 gtttacactc ataagacgtg gattaatacc ccaccatctt ttttctaata cagtaacatc
     8701 actgctgctc ccctgacgtc gaccggacaa gtagagattt ttactatcca ccagcgtggt
     8761 agagttacct tcgatagctt ttagtgtcgc cagatcaaca cgtgacgcgc cgctattact
     8821 ttcggtgcgc tgtccgtcaa aatagaggct atatttatcg accgcgaagc ggcccatagc
     8881 ctgaaagctg gcaatatcga cagtgggtgt tcccggtgga ttgctaacga ttttgccacg
     8941 ccagagaata acgcggccgt ctgtcaccca gtggtattcc tcattttcta tgcttatttc
     9001 atcggaaggt atataacccg ttttcccaga gatatttttc acaaggcgca aaccggccct
     9061 gctcatctcc ggaaactggg caatgatttt cttatccccc caataacctt taccgtaaga
     9121 cataacaccg tgatcattgt aatagtcgaa ccctatggcc tgtacagcag gcgtcagaaa
     9181 tgtgtgtccc aacaggatga gtgcaactgt gattatttga cccttcattt tgtcacctca
     9241 ttgcgcgggg caggaataaa ttcgccgtca tcagtaattt ttccgtccag atatcccgtt
     9301 ttgtaattta cgccatagac atatcccgaa tgttgttcct tccagattaa gcgaacttgt
     9361 ttcggatcag cggtgcggta ttgccagagc tgcgcgccat caaagctgta aatgtagcgg
     9421 ttatcgcggg cataccatct gcgcaaaaag ggatggtcat taagatgggc ttcgcgttca
     9481 tccggagaac ggttatccag taagatcagc ggtccgtcag attcaaacac gtgtaaaccg
     9541 gaatggtatg gctacccttc ggcacgcgta agtaaatagc cgtagcgttt cccggcgttg
     9601 aaacgtttgt tgataataag attgggatca tcaagtttca cgatctccag cttacggtca
     9661 ccagactctg tttcgatgat ggtataaagc gagtcctgat actgggcgac agcatcactg
     9721 atgggttgaa actgctccgg atcgagtccg ggtaattctt cctgtttaca agcaggtcct
     9781 ttacgccaga acactttctt ttccagcaga gaaaaatcca gacagtgctt tgttaaatct
     9841 tcatcccacg ccagattttc cttgtcgagg actttacgct gcagcccgtt tttatcacgc
     9901 caggtcaaga gtgagccggg catccagcgc acgacggaaa aggtgtccgg gtcggcatca
     9961 agctgttcgc cgttgagcag gattttggtc cgtgtacgag ccagggtatc ccaggggcca
    10021 aatggtacgg caatgcaggg gttaaacgtg gtggaaaact ttcctcgctg atcccaggac
    10081 ttctgcgcca gcacacggaa actctcgggg tcagcgaggc gatgcccatt gatatacaag
    10141 aaattagcgt cacgtaatat cagccccagt aagtcaggat cccagggagg ccggaattcc
    10201 acctgatgca gcgttttgat atccaagcta tttccaccac cattgtcatc ggtgcgcttg
    10261 ccttcaaaat agagactgtg tttatctgcg gcaaaatcac cccatgcctt gaacgaggcg
    10321 acatcgacgg gaggtgtgcc gggagggttt tgcacaactt cacccgcgta gaggatattg
    10381 cgaccatcgc tatgccatga ataatgagga tactaggcct gtgcgccctc atagaggtag
    10441 tcggttatca aatcagtcag aggcggttct ttatcccatt cgtctagtgt tgtcgggtct
    10501 attaataaat tgggtaaccg acgcaaactc ttgtaattca cgtcaggtag agaaagattt
    10561 gtttctttat aagggcgcag gtacaccacg ccttttccct gtttctggta aatattatgt
    10621 tcaggtgcgg aaagccgaca tccccatgcc gacgataaaa acaatgaaca agccaacaaa
    10681 caaaccacat tgcgatagtg cataaagcca tcctggcgcg aggtgttgat cacgaaactg
    10741 ccagcagaac ataaatcctc acgagtaagc gttatactcg cagcatttcc tcacttttca
    10801 gacttcataa agagtcgcta aacgcttgct tttacgcctt ctcctgcgat gatagaaagc
    10861 agaaagcgat gaactttaca ggcaatccat aatgaaaaac gtgtttaagg cactcactgt
    10921 attacttact ctcttcagcc tgacgggctg cggtctgaaa ggtccgctct atttcccgcc
    10981 tgcagataaa aacgcaccgc cgccgaccaa accggtagag acgcaaacgc aatccacggt
    11041 gccggataaa aacgatcgcg ccactggcga tggtccatcc caggtgaatt actaaaagtc
    11101 agtttctgta cccgcgtgat tggagtaaat gatgcagttc tcgaaaatgc atggccttgg
    11161 caacgatttt atggtcgtcg acgcggtaac gcagaatgtc tttttttcac cggagctgat
    11221 tcgtcgcctg gcggatcggc acctgggggt agggtttgac cagctgctgg tggttgagcc
    11281 gccgtatgat cctgaactgg attttcacta tcgcattttc aatgctgatg gcagtgaagt
    11341 ggcgcagtgc ggtaacggtg cgcgctgctt tgcccgtttt gtgcgtctga aaggactgac
    11401 caataagcgt gatatccgcg tcagcaccgc caacgggcgg atggttctga ccgtcaccga
    11461 tgatgatctg gtccgcgtaa atatgggcga acccaacttc gaaccttccg ccgtgccgtt
    11521 tcgcgctaac aaagcggaaa agacctatat tatgcgcgcc gccgagcaga caatcttatg
    11581 cggcgtggtg tcgatgggaa atccgcattg cgtgattcag gtcgatgatg tcgataccgc
    11641 ggcggtagaa acgcttggtc ctgttctgga aagtcacgag cgttttccgg agcgcgccaa
    11701 tatcggtttt atgcaagtgg ttaagcgcga gcatattcgt ttacgcgttt atgagcgtgg
    11761 ggcaggagaa acccaggcct gcggcagcgg cgcgtgtgcg gcggtggcgg tagggattca
    11821 gcaaggtttg ctggacgaag aagtacgcgt ggaactcccc ggcggtcgtc ttgatatcgc
    11881 ctggaaaggt ccgggtcacc cgttatatat gactggcccg gcggtacatg tctacgacgg
    11941 atttattcat ctatgaagca accaggggaa gaactgcagg aaacactcac ggagcttgat
    12001 gaccgggcgg ttgtcgatta tctgattaaa aatcctgagt tttttatccg caatgcgcgc
    12061 gcagtagaag cgatacgtgt gccgcatccg gtacgcggca ccgtttcgtt ggtcgagtgg
    12121 cacatggccc gcgcacgtaa tcacattcat gttctggaag agaacatggc gttgttgatg
    12181 gagcaggcta tcgccaacga agggctgttt tatcgcctac tctacctgca gcgcagtctc
    12241 acggccgcca gcagtctcga cgatatgctg atgcgctttc accgctgggc gcgcgatctc
    12301 ggcctggctg gtgcgagtct gcgcctgttt ccagatcgct ggcgcttagg tgcgccgtcg
    12361 aaccacactc atctggcatt aagccgtcag tctttcgaac cgctgcgtat tcagcgtttg
    12421 gggcaggaac agcactatct tgggccgctt aacggaccgg agctgctggt ggtgctaccg
    12481 gaagcgaaag cggtgggatc ggtggcgatg tcgatgctgg gaagcgatgc tgatttgggt
    12541 gttgtgctgt ttaccagtcg cgatgccagt cactatcaac aagggcaggg aacgcagtta
    12601 cttcatgaaa ttgcgctgat gttgccggag cttctggagc gttggattga acgcgtatga
    12661 ccgatttaca caccgatgta gaacgctacc tacgttatct gagcgtggag cgtcagctta
    12721 gcccgataac cctacttaac taccagcgtc agcttgaggc gatcatcaat tttgccagcg
    12781 aaaacggcct gcaaagctgg caacaatgcg atgcagcgat ggtacgcaat tttgctgtac
    12841 gcagtcgccg taaagggctg ggagcagcaa gtctggcgtt acggctttct gcgctacgta
    12901 gcttttttga ctggctggtc agccagaacg aactcaaagc taacccggcg aaaggagttt
    12961 cggcaccgaa agcgccgcgt catctgccga aaaatatcga cgtcgacgat atgaatcggc
    13021 tgctggatat tgatatcaac gatcccctcg ctgtacgcga ccgtgcaatg ctggaagtga
    13081 tgtacggcgc gggtctgcgt ctttccgagc tggtggggct ggatatcaaa cacctcgacc
    13141 tggagtccgg cgaagtgtgg gtgatgggga aaggcagcaa agagcgccgc ctgccgattg
    13201 gtcgcaacgc tgtggcgtgg attgagcact ggcttgattt gcgcgacctg tttggtagcg
    13261 aagacgacgc gctttttctg tcgaaactgg gcaagcgtat ctccgcgcgt aatgtgcaga
    13321 aacgctttgc cgaatggggc ataaaacaag ggctgaataa tcacgttcac ccacataaat
    13381 tacgtcactc gttcgctacg catatgctgg agtcgagcgg cgatcttcgt ggtgtgcagg
    13441 agctactggg tcatgccaac ctctccacca cgcaaatcta tactcatctt gattttcaac
    13501 accttgcctc ggtgtacgat gcggcgcatc cacgcgccaa acgggggaaa taatgcgttt
    13561 ttaccggcct ttggggcgca tctcggcgct cacctttgac ctggatgata ccctttacga
    13621 taaccgtccg gtgattttgc gcaccgagcg agaggcgctt acctttgtgc aaaattatca
    13681 tccggcgctg cgcagcttcc agaatgaaga tctgcaacgc ctgcgccagg cggtacggga
    13741 agcggaaccc gagatttatc acgacgtgac gcgttggcgt tttcgttcga ttgaacaagc
    13801 gatgctcgac gccgggctga gtgccgaaga agccagtgca ggcgcacacg cagcaatgat
    13861 taactttgcc aaatggcgca gccgaatcga cgtcccgcag caaactcacg acaccttaaa
    13921 acagctggcg aagaaatggc cgctggtggc gatcaccaac ggtaacgccc agccggaact
    13981 gtttggtttg ggggattatt ttgagtttgt gctgcgcgct ggcccgcacg ggcgctcaaa
    14041 accgttcagc gatatgtact ttttggctgc ggaaaaactc aacgtgccga tcggcgagat
    14101 cttacatgtt ggggacgatc tcaccactga cgtgggtggg gcaattcgca gcggaatgca
    14161 ggcttgttgg atcagaccgg aaaatggcga tctgatgcaa acctgggaca gccgtttact
    14221 gccacatctg gaaatttccc ggttggcatc tctgacctcg ctgatataat cagcaaatct
    14281 gtatatatac ccagcttttt ggcggagggc gttgcgcttc tccgcccaac ctatttttac
    14341 gcggcggtgc caatggacgt ttcttacctg ctcgacagcc ttaatgacaa acaacgcgaa
    14401 gcggtggccg cgccacgcag caaccttctg gtgctggcgg gcgcgggcag tggtaagacg
    14461 cgcgtactgg tgcatcgtat cgcctggctg atgagcgtgg aaaactgctc gccatactcg
    14521 attatggctg tgacgtttac caacaaagcg gcggcggaga tgcgtcatcg tatcggacaa
    14581 ctgatgggca ccagccaggg cggcatgtgg gtaggcacct tccacgggct ggcgcaccgc
    14641 ctgctgcgtg cgcaccatat ggacgccaat ctgccgcagg atttccagat cctcgatagc
    14701 gaagaccagc tacgcctgct taagcgtctg atcaaagcca tgaacctcga cgagaagcag
    14761 tggccgccgc gccaggcaat gtggtacatc aacagccaga aagatgaagg cctgcgtccg
    14821 catcatattc aaagctacgg taatccggtg gagcagacct ggcagaaggt gtatcaggcg
    14881 tatcaggaag cgtgcgatcg cgcgggcctg gtggacttcg ccgagctgct gctgcgcgct
    14941 cacgaactgt ggcttaacaa gccgcatatc ctacaacact accgcgaacg ttttaccaat
    15001 atcctggtgg acgaattcca ggataccaac aacattcagt acgcgtggat ccgcctgctg
    15061 gcgggcgaca ccggcaaagt gatgatcgtc ggtgatgacg accagtcaat ctacggctgg
    15121 cgcggggcgc aggtggagaa tattcagcgt ttccttaatg atttccccgg tgccgaaatt
    15181 attcgtctgg agcaaaacta ccgctctacc agcaatattc tgagcgccgc taacgccctg
    15241 attgaaaaca ataacgggcg tctgggtaaa aaactgtgga ccgatggcgc ggacggtgag
    15301 cctatttccc tctattgcgc ttttaacgaa ctcgatgaag cgcgttttgt ggttaaccgc
    15361 atcaaaacct ggcaggacaa cggcggggcg cttgccgagt gcgccattct ctaccgcagt
    15421 aacgcccagt cgcgtgtgct ggaagaagca ctgttgcagg cgagtatgcc gtaccgtatt
    15481 tacggcggga tgcgcttctt cgaacgccag gaaatcaaag atgcgctctc gtatctgcgc
    15541 ctgattgcca accgtaacga cgacgcggcc tttgagcgtg tggtgaatac gccaacgcgg
    15601 ggtattggtg accggacgct ggacgtggta cgtcagacat cgcgcgatcg ccagttaaca
    15661 ctctggcagg catgtagtga actgttgcag gaaaaagccc tcgccggacg tgctgccagc
    15721 gccttacagc ggtttatgga actgatcgac gccttagcgc aggaaactgc cgatatgccg
    15781 ctgcatgtac agactgaccg ggtaattaaa gactccggcc tgcgtaccat gtacgagcag
    15841 gagaagggcg aaaaaggtca gacgcgtatc gaaaacttag aggaactggt gacggcaacg
    15901 cggcagttca gctacaacga agaagacgaa gatttaatgc cgctgcaggc gttcctatcc
    15961 catgcggcgc tggaagcagg cgaagggcag gcggatacct ggcaggatgc ggtacaactg
    16021 atgaccatgc actcggcgaa aggtctggag ttcccgcagg tgtttatcgt cggtatggaa
    16081 gagggcatgt tcccaagcca gatgtcgctg gatgaaggcg ggcgtctgga agaagaacgc
    16141 cgtctggcct acgttggcgt aacccgcgcg atgcagaaac tgacgctgac ctacgcggaa
    16201 actcgccgtc tgtatggcaa agaggtttac catcgcccgt cgcgctttat tggcgagttg
    16261 ccggaagagt gtgtggaaga ggtgcgcctg cgcgccacgg taagccgccc ggtcagccat
    16321 cagcggatgg gtacgccgat ggtcgagaac gacagcggct acaagctcgg ccagcgtgta
    16381 cgccacgcta agtttggtga aggcaccatc gtcaatatgg aaggcagcgg tgaacatagc
    16441 cgtttgcagg tggcattcca gggccaggga atcaaatggc tggtggcagc ttacgcccgg
    16501 ctggagtcgg tgtaacgttg ccggatgcgg tgctgcgcac cttatttggc ctaaaaaatc
    16561 attcagattc aataaattgc aacgtcatgt aggcaggata aggcgtttac gccgcatccg
    16621 gcatcagcag catatttagt atctgacaca aagctatcat tctaaccttt ccgctcgacg
    16681 gaaatcatgg tgacaaaagg atagcgttgc cacgggattg ccccgccttt catatacata
    16741 tgtgaaatcg tgccaccaag ataaagcagc tgctcaacgt tcagtttcgc tttggcataa
    16801 caggcaaaat cataaaaatt tgtcgcctgc tggctcaaca aaaacacggc attcccatgt
    16861 ttattaatcc caacaccgtt acgaatttta cgtgaggcga cgttgggatg aatacgcgga
    16921 ttaattacac cgttttccat caacattggt cctgactgca ccgcaaactg aatatcttta
    16981 ctggctttaa aagcatccag acgaacgatg ccgactttat ctcccgcgac ataaaacacg
    17041 ccgccaggac ggataaagaa attcccttca cctgaagcga gatttaacgc taccttctg
//
LOCUS       DAJTYG010000079        16616 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTYG010000079 DAJTYG010000000
VERSION     DAJTYG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.079"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OO568_002852"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OO568_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6642136.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTMSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVHNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OO568_002853"
     CDS             2219..2767
                     /locus_tag="OO568_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6642137.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OO568_002854"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OO568_002854"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6642138.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QSLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO568_002855"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO568_002855"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6642139.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFSAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO568_002856"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO568_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6642140.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OO568_002857"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OO568_002857"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6642141.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8298..9500)
                     /gene="pncB"
                     /locus_tag="OO568_002858"
     CDS             complement(8298..9500)
                     /gene="pncB"
                     /locus_tag="OO568_002858"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6642142.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9766..12378
                     /gene="pepN"
                     /locus_tag="OO568_002859"
     CDS             9766..12378
                     /gene="pepN"
                     /locus_tag="OO568_002859"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6642143.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12585..13352)
                     /gene="ssuB"
                     /locus_tag="OO568_002860"
     CDS             complement(12585..13352)
                     /gene="ssuB"
                     /locus_tag="OO568_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6642144.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLFDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13349..13996)
                     /locus_tag="OO568_002861"
     CDS             complement(13349..13996)
                     /locus_tag="OO568_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6642145.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNI
                     DRGLVEMARSYGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGI
                     GYLAMNAREFLQTDVVVVAIILYALLGKLADVSAQLLERLWLSWNPAYHLKEATI"
     gene            complement(14008..15153)
                     /gene="ssuD"
                     /locus_tag="OO568_002862"
     CDS             complement(14008..15153)
                     /gene="ssuD"
                     /locus_tag="OO568_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR6642146.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSEHYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15150..16109)
                     /gene="ssuA"
                     /locus_tag="OO568_002863"
     CDS             complement(15150..16109)
                     /gene="ssuA"
                     /locus_tag="OO568_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6642147.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPAGARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16102..>16616)
                     /gene="ssuE"
                     /locus_tag="OO568_002864"
     CDS             complement(16102..>16616)
                     /gene="ssuE"
                     /locus_tag="OO568_002864"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR6642148.1"
                     /translation="YAREKLNGLDVEVYHWNLQNFAPEDLLYARFDSPALKTFTEQLQ
                     QADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVVLPLATGGTVAHLLAVDYALKP
                     VLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQPHLDTALETFWQALHRRDVQVPD
                     LLSLRGNAHA"
BASE COUNT         4144 a         4291 c         4254 g         3927 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga tgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cacaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgctctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agtcgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgcctctgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg agaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcag ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaattatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgtaaccaa aagtaaaaat taaggagctt tgtgaacacc
     6361 gtcatatttc catagaaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aacgaatccg caataagaat gcgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcgggggt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgtgcgaaat cggcttcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atggggggga tacctatgtt acctggcatc tgcaatcaga
     8221 caagcagaat tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa agggagccga
     8281 ttagctccct gatgatatta actggctttt ttaatatgcg gaaggtcgaa cgctttgcgc
     8341 agcgcccgaa caaacgcttt atcatggcag atagttttgc cagggctgtc agaaagtttc
     8401 gccaccggtt taccgttaca ctctaccaac ttaatcacaa tattcagggg ttttacctgg
     8461 gggatatcgc aggtcaggcg agtcccaata ccaaaactta attgcacgcg ggaagagaag
     8521 tggcggtata gctcaaccgc tttgcgtaaa tccagattgt cagagaaaac cagcgtttta
     8581 ctctgtggat caattcccag cttttcataa tgtgcaatgg ctttttcacc ccattcaacc
     8641 gggtcgccag agtcatgacg caggccctga taccgactag cgaactcgac accgaaatca
     8701 cgcaggaaag catccatagt gatgcagtcg gttaatgcaa tgccaagttg gtcgggatac
     8761 tcttccagcc aggcagcaag tgcagctcgc tggctgttgg ctagatccgg gctgatttgc
     8821 tgatgtgcct ggaaccattc gtgtgcctgt gttcccatcg gcgtgaggga aagccgacgc
     8881 gccagatcgt agttgctggt gcccacgaac caggattcct gttgcagacg cttaacgatg
     8941 gtttcttgta cttcgcgaga aaaacggcga cgggtgccaa aatccatcag atggaagcgc
     9001 gacatatcaa gaccggcggt taacgccgag aagtcgacta atttgctttc cagcgtgtcg
     9061 agggcttgtg caacgtcggc ctgcggtgag cgatagcgat gtaccatttc actgataacc
     9121 gccagcaaag gaacttccca gaggatgact tcacgccacg gaccgcttaa gcgaatatcc
     9181 agcttgccat tatcgttgga cacggtgact tgttccgggt taaagcggaa ctcgcgtaac
     9241 cagttaagat agtcggcctt aaagaaaggc agggcagaga gccactgata ttcatcatcc
     9301 tgcaggcgca ggtgctgcat cgcctgaatc tgttcacgaa tagcatcggc ataaataccc
     9361 agcagatcgt cacctcggca acgaaactcc gccgcgacat gcacatcgta atagtgatga
     9421 aacacggctt gctgcatatg caacttataa gcatctgtat ccagcaacga gtgcagaaca
     9481 ggagaagcga attgtgtcat aggtgcgcag tagcgtcctc gtacaggagc gtttagtaca
     9541 ataaacatct tcaggaactg ctggagtata ccttgtttcg caatttattg aaccccgatc
     9601 acaccatatg ccacctttct ggtcgatggc attacgcggt gtatgttata aaaatgtagc
     9661 aataaaggcg tttgtacctg aaaagatgaa gattctgcat agcgcgattt acgcaacagg
     9721 aatagactga acaccagact ctataaaaga tgctaaaggt tatttatgac tcaacagcca
     9781 caagccaaat accgtcacga ttatcgtgcg ccggattacc agattactga tattgacttg
     9841 acctttgacc tcgacgcgca aaagacggtc gttaccgcgg tcagccaggc tgtccgtcat
     9901 ggtgcatcag atgctccgct tcgtctcaac ggcgaagacc tcaaactggt ttctgttcat
     9961 attaatgatg agccgtggac cgcctggaaa gaagaagagg gcgcactggt catcagtaat
    10021 ttgccggagc gttttacgct taagatcatt aatgaaataa gcccggcggc gaatacggcg
    10081 ctggaagggc tttatcagtc aggcgatgcg ctttgcaccc agtgtgaagc cgaaggtttc
    10141 cgccatatta cgtattatct cgaccgcccg gacgtgctgg cgcgttttac caccaaaatt
    10201 attgccgata aaatcaaata tcccttcctg ctttccaatg gtaaccgcgt tgcgcaaggc
    10261 gaactcgaaa acggacgcca ttgggtacag tggcaggacc cgttcccgaa accgtgctac
    10321 ctgtttgcgc tggtggcagg cgactttgat gtactgcgcg atacctttac cacgcgttct
    10381 ggtcgcgaag tagcactgga gctgtacgtc gatcgcggca accttgatcg cgcgccgtgg
    10441 gcgatgacct cgctgaaaaa ctccatgaaa tgggatgaag aacgctttgg cctggagtat
    10501 gacctcgaca tctatatgat cgtcgcggtg gatttcttca atatgggcgc aatggagaat
    10561 aagggtctga atatctttaa ctccaaatat gtgctggccc gcaccgacac cgccaccgac
    10621 aaagattacc tcgatattga acgcgttatc ggccatgaat atttccataa ctggaccggt
    10681 aaccgagtga cctgccgcga ctggttccag ctcagcctga aagaaggttt aaccgtcttc
    10741 cgcgatcagg aattcagctc tgaccttggt tcccgcgcag ttaaccgcat caataatgtg
    10801 cgcaccatgc gcggattgca gtttgcggaa gacgccagcc cgatggcgca cccaatccgc
    10861 ccggacatgg tcattgagat gaacaacttc tacaccctga ccgtttacga gaagggcgcg
    10921 gaagtgatcc gtatgatcca caccctgctg ggcgaagaaa acttccagaa agggatgcaa
    10981 ctctatttcg agcgtcatga tggtagcgca gcgacctgtg acgactttgt gcaggcgatg
    11041 gaagatgcgt cgaatgttga tctctcccat ttccgccgtt ggtacagcca gtccggtacg
    11101 ccggttgtga ccgtcaaaga cgactacaat ccggaaactg agcagtacac cctgaccatc
    11161 agccagcgca cgccagccac gccggatcag gcagaaaaac agccgctgca tattccgttt
    11221 gccatcgaat tgtatgacaa cgaaggcaaa gtgatcccgt tgcagaaagg cggtcatccg
    11281 gtgaattccg tgctgaacgt cacccaggcg gaacagacct ttgtttttga taatgtctac
    11341 ttccagccgg tgcctgcgct gctgtgcgaa ttctctgcgc cagtgaaact ggaatataag
    11401 tggagcgatc agcaactgac cttcctgatg cgtcatgcgc gtaatgattt ctcccgttgg
    11461 gatgcggcgc aaagtttgct ggcaacctac atcaagctga acgtcgcccg tcatcagcaa
    11521 gggcagccgc tgtctctgcc ggtacatgtg gctgacgctt tccgcgcggt gctgctcgat
    11581 gagaagattg atccggcgct ggcggcagaa atcctgacgc tgccttctgt caatgaaatg
    11641 gctgaactgt tcgatatcat agacccgatt gctattgccg aagtacgcga agcactcact
    11701 cgtactctgg cgactgaact ggcggatgag ctgctggcta tttacaacgc gaattaccag
    11761 agcgagtacc gtgttgagca tgaagatatt gcaaaacgca ctctgcgtaa tgcctgcctg
    11821 cgcttccttg cttttggtga aacgcatctg gctgacgtgc tggtgagcaa gcaataccat
    11881 gaagcaaaca atatgaccga tgcgctggca gcgctttctg cggcggttgc tgcacagctg
    11941 ccttgccgtg acgcgctgat gcaggagtac gacgataagt ggcatcagga cggtctggtg
    12001 atggataaat ggtttatcct gcaagccacc agcccggcgg cgaatgtgct ggagacggtg
    12061 cgtggtctgt tgcagcatcg ctcatttacc atgagcaacc cgaaccgtat tcgttcgttg
    12121 attggtgcgt ttgcgggcag caacccggca gcgttccatg ccgaagatgg cagcggttac
    12181 cagttcctgg tggaaatgct taccgacctc aacagccgta acccgcaggt agcttcacgt
    12241 ctgattgaac cgctgattcg cctgaaacgt tatgatgcca aacgtcagga gaaaatgcgc
    12301 gcggcgctgg aacagttgaa agggctggaa aatctctctg gcgatcttta cgagaagata
    12361 actaaagcac tggcttgata cttttgagcc aaaatgcctg atgcgacgct tgacgcgtct
    12421 tatcaggcct acaactccgt tgaaattgct ggttttgtag gctggataag gcgttcacgc
    12481 cgcatccggc agccgtgccc aactgcctga tgcgatgctt gacgtgtctt atcaggccta
    12541 caagtccgtt gaaaatgctg gttttgtgcg ccacatccgg catattaacc atgacgccga
    12601 atcagttgtt cagaatgccc acgccgcatt acccgttgca atacttccgc ttccagctcc
    12661 gctaatcgca ccgaccctaa tcgacgcgga cgggggatat ccactgtcaa atccagacta
    12721 atttttccct cttcaattaa caacacccgg tcagccattg ctaccgcttc gctcacatca
    12781 tgcgtcacca acagtacggt aaagccgtgc tgctgccaaa gtgacacaat caaatcctgc
    12841 atctcgagtc gcgttaaggc gtccagtgcc ccgagcggtt catcaaacaa caataaaccc
    12901 ggtcgatgaa tcaacgctct tgccagcgcc acacgctgtt tctgcccgcc agaaagtgcg
    12961 gcaggccatt ccccggcgcg attctccagt cctaccgcag ccaacgcccg acgcgcggca
    13021 tcgcgccact gacctttaag gcctaaccca acgttatcaa tcaccgattt ccacggcagc
    13081 agacgcgcat cctgaaacat cattcgcgta tcatcctgaa tttcagccag cggtgtggtg
    13141 cccgctaaca catcgcctgc ggttggcgtt tccagaccgg ccagcaggcg cagcagggta
    13201 cttttaccac caccgctgcg gcccaccacc gccacaaact gacctgccgg aatatgtaaa
    13261 tccagttggt tcaggacgat attttccgcg taatgtttgc ttactgcatt gagcaacaat
    13321 ggtgtgccct ggttcagacg agcagtattc atatcgtggc ctccttcaaa tgataagccg
    13381 ggttccagct caaccagagg cgctctaaca gctgcgcgct gacgtcagca agtttgccga
    13441 gcagggcgta aagaataata gcgactacca ccacgtctgt ttgcaaaaac tctcgcgcat
    13501 tcatcgccag ataaccaatg ccggaattgg cagaaatggt ttcggcaaca atcagcgtca
    13561 gccacatcag gcccaacgca aaacgcacgc cgaccataat tgagggcagg gcatcgggca
    13621 ggatcacatg gataaacagc ggtatgccgg ataatccata gctacgcgcc atctccacca
    13681 gcccgcgatc gatattacgg atgccatgcc aggtgttgat ataaatgggg aacagcgtac
    13741 cgagcgccac cagaaagatt ttcgcggatt catcaatgcc aaaccacaaa atcaccagcg
    13801 ggatcagcgc cagatgctgc cacagttcgc cgctggcgga gagcgtccag aacgccgtca
    13861 ccactccttc cggtgaaggc aaaatacgcg tcgacagcca gccaaccgag gaggccagtt
    13921 gccacaccgc cacgatgccc accggtaaaa accagggggc aacgcgcaat aaccacttct
    13981 tcactggcgt tgccatcatc gtgttcctta gctttgcgcg actttacggg ggataaaatc
    14041 attcgccacc gcttcgcctt gcggattcag cggctgcggc tggggaattt ccggaatggc
    14101 gacatccaga tgtgggaaca gcaactcgcc aacccgatac gcttcttcca gatgcgggta
    14161 gcccgaaagc acaaagctgt cgatgccaag cgcggcgtat tcgttgattc gcgcggcgac
    14221 cgtaggacca tcgcccacca gcgccgttcc ggcaccgccg cgcactaagc caacgcccgc
    14281 ccataaattg gggctgatct ccagattgtc gcgcttgccg ttatgtagcg ccgccattcg
    14341 ctgttgtcct acggaatccg tccgggcgaa tgcggcttgt gctttggcga tagtctcatc
    14401 atcaagatgc gagattaacc gctcggcggc ctgccacgct tcgtcgttag tttcacgaac
    14461 aatcacatgc agacgaatac cgaaacgaat tttgcgccca tacgcggcag ctttcgcccg
    14521 cacttgttcg attttctctt taacaagttc cggcggttcg ccccaggtga ggtagagatc
    14581 aacttgttct gccgccagct cctgggcgac atctgacgat ccgccaaagt aaagcggagg
    14641 ataaggctgt tgaatcggcg ggaagagcag ttttgctccg cgcacatgaa tatgtttacc
    14701 gttgaaattg acggtttccc ccagcaataa gcgccgccag acctgggtaa attccgccga
    14761 ggcttcgtag tgctcgctat gatcaaggaa cacgccgtcg cctgccagct cttgtggatc
    14821 gctgcctgtg accagattaa acaacgcacg accatttgag agacggtcaa gcgtggcggc
    14881 ctggcgggcg gcaacagtag gtgaggttac gctgggacgc agggcgacaa gaaacttcag
    14941 ccgctgcgtc accgggatca tcgacgctgc aaccagccac gcatcttcac acgatcgccc
    15001 cgttgggatc agcacaccgg tatagcccag acgatccgcc gcttgcgcaa tttgttgcag
    15061 ataaccgtga tcaaccgggc gtgaaccttc ttccgttccc agatagtgcc cgtcaccgtg
    15121 ggtcggtaaa aaccagaaca tattcagact cataattgtt ttccttccag ttgagtgggc
    15181 tgccagatgc gctggcgaat atcgactttt ttcggcacca gacggttttc ataaaacaga
    15241 tctgccgttt gctgctgtaa ggcggcaacc tcggcgttaa ccggtttgat ggtggtagga
    15301 gggcgatgat ctaagtaaga ggcaatcacc ggtgccggta agcccatcgt ttttgccagt
    15361 aaagcgatgc tttgttcgcg ctggctgcgg gttaacgcat cggcctcact aaaggttgcc
    15421 agtacgccct gaataaaagc gccgtttttt tctgcatagg gacgagctgc cagataaaac
    15481 gatccagttt gattgagatc ggtgccgtct ttcagcaccc gcacgccgcc ctgtaataac
    15541 gcagcggagt agtagggatc ccagatagcc caggcgtcaa cgttaccttg ctggaacgcg
    15601 gcgcgggcac cggcaggcgt caggtaagtg ggctggatat cggtgaattt aagtccggcc
    15661 tgacgcagtg cacgcagtaa aaggttgtgt gaactggaac ctttctgaaa ggcaactttg
    15721 tgacctttaa gatcggctac ggttttgatc gggctgtttt ctgccaccag aatcacttcg
    15781 gctttgggct tcggtggctc gacgcccaca tataccaaat cagccccggc agcctgggca
    15841 aagattggcg gaatatcccc ggtactgccg agatcaatac tgccaacgtt taacgcttcc
    15901 aacatttgcg gacccgcggg gaactccacc caggagattt ttgattgcgg atagcgtttt
    15961 tccagtaact ggtggctttt tgccagcacc ataccaatac tgcctttctg atagcctata
    16021 cgtaacgctt caggcgagga ttctgcagca accgcaaaag tagagacgct aagcaatccc
    16081 gccagcgcca gtttaatgat gttacgcatg ggcattacct cgcagagaca gaaggtcagg
    16141 aacctgaaca tcgcggcggt gcaatgcctg ccagaaagtt tctagcgcgg tatcaagatg
    16201 gggttgcaga tttggcgtga actggggtct gtgatggtaa tcaattaatt gtgagtcatc
    16261 ggcaaacacg ccgtgcagga tctcctgagc tttcagtgcg cttaaaactg gtttaagggc
    16321 ataatcgacc gccagcagat gggccacggt accgcccgtc gccagcggta gcaccacttt
    16381 gccttgcaaa gcgcgctctg gcagcaggtc gagcagggtt ttcagcgcgc cggaataggc
    16441 ggctttatac acaggcgtgg cgacaatcag cccatcagcc tgttgcagct gttcggtgaa
    16501 ggtcttgagt gccggactat cgaaacgagc ataaagcaga tcttccgggg cgaagttttg
    16561 cagattccag tgataaacct ctacatctag gccatttagt ttttcccgcg catatt
//
LOCUS       DAJTYG010000080        16325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTYG010000080 DAJTYG010000000
VERSION     DAJTYG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.080"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(88..2064)
                     /gene="speA"
                     /locus_tag="OO568_002865"
     CDS             complement(88..2064)
                     /gene="speA"
                     /locus_tag="OO568_002865"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6642149.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENALPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2073..2204)
                     /gene="yqgB"
                     /locus_tag="OO568_002866"
     CDS             complement(2073..2204)
                     /gene="yqgB"
                     /locus_tag="OO568_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6642150.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2340..2555
                     /locus_tag="OO568_002867"
     CDS             2340..2555
                     /locus_tag="OO568_002867"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642151.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2859..4013
                     /gene="metK"
                     /locus_tag="OO568_002868"
     CDS             2859..4013
                     /gene="metK"
                     /locus_tag="OO568_002868"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6642152.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4450..5844
                     /gene="galP"
                     /locus_tag="OO568_002869"
     CDS             4450..5844
                     /gene="galP"
                     /locus_tag="OO568_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6642153.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5921..6418
                     /locus_tag="OO568_002870"
     CDS             5921..6418
                     /locus_tag="OO568_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6642154.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLAWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6513..7220
                     /gene="endA"
                     /locus_tag="OO568_002871"
     CDS             6513..7220
                     /gene="endA"
                     /locus_tag="OO568_002871"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6642155.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7300..8031
                     /gene="rsmE"
                     /locus_tag="OO568_002872"
     CDS             7300..8031
                     /gene="rsmE"
                     /locus_tag="OO568_002872"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6642156.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8044..8994
                     /gene="gshB"
                     /locus_tag="OO568_002873"
     CDS             8044..8994
                     /gene="gshB"
                     /locus_tag="OO568_002873"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6642157.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9103..9666
                     /locus_tag="OO568_002874"
     CDS             9103..9666
                     /locus_tag="OO568_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6642158.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9666..10082
                     /gene="ruvX"
                     /locus_tag="OO568_002875"
     CDS             9666..10082
                     /gene="ruvX"
                     /locus_tag="OO568_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6642159.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10258..11238)
                     /locus_tag="OO568_002876"
     CDS             complement(10258..11238)
                     /locus_tag="OO568_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6642160.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11256..11960
                     /gene="yggS"
                     /locus_tag="OO568_002877"
     CDS             11256..11960
                     /gene="yggS"
                     /locus_tag="OO568_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6642161.1"
                     /translation="MNDIAHNRAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYIRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11978..12544
                     /gene="yggT"
                     /locus_tag="OO568_002878"
     CDS             11978..12544
                     /gene="yggT"
                     /locus_tag="OO568_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6642162.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12541..12831
                     /locus_tag="OO568_002879"
     CDS             12541..12831
                     /locus_tag="OO568_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6642163.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12839..13432
                     /locus_tag="OO568_002880"
     CDS             12839..13432
                     /locus_tag="OO568_002880"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6642164.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAEMTREEKSAISHRGQALKLLLDALRNG"
     gene            13425..14561
                     /gene="hemW"
                     /locus_tag="OO568_002881"
     CDS             13425..14561
                     /gene="hemW"
                     /locus_tag="OO568_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6642165.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRSRLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGMQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFIAE"
     gene            14875..15838
                     /locus_tag="OO568_002882"
                     /pseudo
     CDS             14875..15838
                     /locus_tag="OO568_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15883..>16325
                     /locus_tag="OO568_002883"
     CDS             15883..>16325
                     /locus_tag="OO568_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR6642166.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYA"
BASE COUNT         4042 a         4206 c         4111 g         3966 t
ORIGIN      
        1 aagcagctag attattttta agaatatata aaaagaaagc gggccgattt acgtaaataa
       61 atcgacccgc cagcggaata atcttaatta ttcatcttca agataagtat aaccgtacaa
      121 acctgcctcg aactcttcaa ggaactgttg ttgcagttca gcatcaagat cggttttctt
      181 cacttgatcg cggaactggg ttaacagcgt tttcggatcg agctgtacat attgcagcat
      241 gtccgccacg gtatcgcctt cgtcagacag ttctacttct acgctaccgt cagggaacac
      301 gaacacgtca actgcttcgg tatcaccgaa caggttgtgc atattgccaa ggatctcctg
      361 atatgcaccg accataaaga aaccgagcat cggcggattc tctggatcgt actccggcat
      421 tggcattgtc gtggcaatac catcaccatc aatatagtgg tcgatagcac cgtcagagtc
      481 acaggtaata tccagcagca cagcgcgacg ttccggcact tgatccagcc cttccagcgg
      541 tagaaccggg aacaactggt cgatccccca tgcgtccggc attgactgga acagcgagaa
      601 attgacgtac attttgtccg ccatacgttc ctgcagttcg tcgattatcg gacgatgcgc
      661 acggttttgc ggatccagtt gcttctgcac ttcatggcac atgctcagat agagttgttc
      721 tgcccatgca cgttcttgca ggctaaaggt gccggaagag tagccgatat gaatgtcgtg
      781 cagatccatc tggctgtcgt gtaaccattc acgcaaagaa cggcgagttc ccggttcgtg
      841 catttcctgc caggtttccc acatgctttg cagcgcacgc ggcgcatctt ctgcgggcgc
      901 ggtcggcacc gtatattcgt tacgttccac gccgatgata ttcgacacca gcacggtatg
      961 gtgcgcagtc accgcacggc ccgattcggt gattaccgtc ggatgcggca gagcattttc
     1021 ttcacacgca tcgccaatcg cccagatgat gttgttggcg tattcattga ggccatagtt
     1081 tacggagcag tcagactgcg aacgggtgcc ttcataatcc acgcccagac cgccgccgac
     1141 gtcgaagcac tgaatattga cgcccagctt gtgcagttct acatagaaac gcgcggattc
     1201 acgaacgcct gtcgcgatat cgcgaatatt cgccatctgc gaaccgaggt ggaagtgcag
     1261 tagttgcagg ctgtcgagac gcccggcttc acgcagggtt tcaaccagtt gcagtacctg
     1321 agtcgcagcc aggccgaact tcgatttttc cccgccggag gactgccatt tacccgagcc
     1381 ctgcgaagcc agacgtgcac gcacgcccag acgaggaacg acattcagac gttctgcttc
     1441 atccagcaca atggcgattt ctgacatctt ctcaatgacc agatagacct tgtgccccat
     1501 cttctcgcca attaatgcca ggcggatata ttcgcggtct ttataaccgt tgcagacgat
     1561 gacgctacga gtcatgccag catgtgccag cactgccatc aactcggctt tggaaccggc
     1621 ttccagaccc agcggttcac ccgaatgaat cagggactcg atcacgcggc ggtgctggtt
     1681 aactttgatc ggataaacaa ggaagtaatc gccgttatag ccgtaggatt ccctcgcacg
     1741 tttgaacgcg gcgttaatgg aacgcaaacg gtgctgcagg atctgtggga aacagaacag
     1801 tgcaggcaga cgctgaccct gtgcttcacg agttttcact aactgcgcga gatcgacgcg
     1861 agcttccggg acgtccgggt ccgggcacac gctgatgtgg cccagctcgt taacgtcata
     1921 gtagttattg ccccaccagg caatattgta agtacgcagc atcttgctgg cttcctggga
     1981 gctcattgca acctcctgca tggagcgtag tacaccgtgt tcgcccgctg acgaaggcaa
     2041 acccatagac atgtcgtcag acatagcgaa cctcaaatta ttttattaag tgtaaaacag
     2101 ttaacgactg tcgcagcctg gaactgcgat aacaacccat aagcacatgg attttccagc
     2161 agtgtatgct gacgctccga ctgcgcaacc ggtttctttt tcataacatt attaagcaca
     2221 taaccgaacg taagtgtgac agttcggcga aaccacgaga aaactcttgt ttttacaaga
     2281 gcgcccttgt tcagtcctca gtaactgtaa ccagctcttg aatcctgaga agcgccgaga
     2341 tgggtataac atcggcaggt atgcaaagca gagatgcaga gtgcggggaa cgaatcttca
     2401 ccagaacggt gagacaggtt aagcagcaga caacggttca ttatttcgta tcacctccac
     2461 ggccgcctgt taagacgaac ccacaagcca aaactctgat ttcaacccgg ctggaagtgg
     2521 caacacgaaa aaaacgtcgt gtgcttttta tttaagccgc gcgccgcgtt ttatacccca
     2581 caatggcaga aaattgcaaa agataaatac gcagaatgcc ggcattgtca ggaaaaattt
     2641 ccagccacgt ttttaacaga atgagacacg attcaaaaaa aagtggaaat cgggtgaaga
     2701 attgacctaa aatagccatc cagatgttaa tccatccata ccgattaaca ctcagactgc
     2761 cagtgttttt aacctgcaga gtcgtggtag gatccgctac cacagaaaat ccacacaaca
     2821 gtttgagcta accaaattct ctttaggtga tattaaatat ggcaaaacac ctttttacgt
     2881 ccgagtccgt ctctgaaggg catcctgaca aaattgctga ccaaatctct gatgccgttt
     2941 tagacgcgat cctcgaacag gatccgaaag cacgcgttgc ttgcgaaacc tacgtaaaaa
     3001 ccggcatggt tttagttggc ggcgaaatca ccaccagcgc ctgggtagac atcgaagaga
     3061 tcacccgtaa caccgttcgc gaaattggct atgtgcattc cgacatgggc tttgacgcta
     3121 actcctgtgc ggttctgagc gctatcggca aacagtctcc tgacatcaac cagggcgttg
     3181 accgtgccga tccgctggaa cagggcgcgg gtgaccaggg tctgatgttt ggctacgcaa
     3241 ctaatgaaac cgacgtgctg atgccagcac ctatcaccta tgcacaccgt ctggtacagc
     3301 gtcaggctga agtgcgtaaa aacggcactc tgccgtggct gcgcccggac gcgaaaagcc
     3361 aggtgacttt ccagtatgac gacggcaaaa tcgttggtat tgatgctgtc gtgctttcca
     3421 ctcagcactc tgaagagatc gaccagaaat cgctgcaaga agcggtaatg gaagagatca
     3481 tcaagccaat tctgcccgct gaatggctga cttctgccac caaattcttc atcaacccga
     3541 ccggtcgttt cgttatcggt ggcccaatgg gtgactgcgg tctgactggt cgtaaaatta
     3601 tcgttgatac ctacggcggc atggcgcgtc acggtggcgg tgcattctct ggtaaagatc
     3661 catcaaaagt ggaccgttcc gcagcctacg cagcacgtta tgtcgcgaaa aacatcgttg
     3721 ctgctggcct ggccgatcgt tgtgaaattc aggtttccta cgcaatcggc gtggctgaac
     3781 cgacctccat catggtagaa actttcggta ctgagaaagt gccttctgaa caactgactc
     3841 tgctggtacg tgagttcttc gacctgcgcc catacggtct gattcagatg ctggatctgc
     3901 tgcacccgat ctacaaagaa accgcagcat acggtcactt tggtcgtgaa catttcccgt
     3961 gggaaaaaac cgacaaagcg cagctgctgc gcgatgctgc cggtctgaag taatctttct
     4021 tcacctgcgt tcaaaggcca gcctcgcgct ggcctttttc ttttacctca ccgcggtgga
     4081 taggcgttca cgccgcatcc agcaaaaaaa ctgcccgcac aataacatca ttcttcctga
     4141 tcacgtttca cggcaaatta ccatcacaat tgaaaccgat tacaccaacc acaccagcaa
     4201 aatatttgta atattttcat attattattc agctttcaca gttgttacat ttcttttcag
     4261 taaagtctta attgcagatg acagcgttta atctatgatg atataactca attattttca
     4321 tgcacttaaa tcataactaa gataaatgtt agtgtaagcg attacactga tgtgatttgc
     4381 ttcacatctt tttacgtcgt actcacctat cttaattcac aataaaaaat aaccatattg
     4441 gagggcatca tgcctgacgc taaaaaacag gggcggtcaa acaaggcaat gacgtttttc
     4501 gtctgcttcc ttgccgctct ggcgggatta ctctttggcc tggatatcgg tgtaattgct
     4561 ggcgcactgc cgtttattgc agatgaattc cagataactt cgcacacgca agaatgggtc
     4621 gtaagctcca tgatgttcgg tgcggcagtc ggtgcggtgg gcatcggctg gctctccttt
     4681 aaactcgggc gcaaaaagag cctgatgatc ggcgcaatcc tgtttgttgc cggttcactg
     4741 ttctctgcgg ctgcgccaaa cgttgaagta ctgattcttt cgcgcgttct gctggggctg
     4801 gcggtgggtg tggcctctta taccgcacca ctgtacctgt cagaaattgc gccggaaaaa
     4861 attcgcggca gtatgatctc gatgtatcag ttgatgatca ctatcgggat cctcggtgct
     4921 tatctttctg ataccgcctt cagctacacc ggtgcatggc gctggatgct gggtgtgatt
     4981 atcatcccgg caattttgct gctgattggt gtcttcttcc tgccagacag cccacgttgg
     5041 tttgccgcca aacgccgttt tgttgatgcc gaacgcgtgc tgctacgcct gcgtgacacc
     5101 agcgcggaag cgaaacgcga actggatgaa atccgtgaaa gtttgcaggt taaacagagt
     5161 ggctgggcgc tgtttaaaga gaatagcaac ttccgccgcg cggtgttcct tggcgtactg
     5221 ttacaggtaa tgcagcaatt caccgggatg aacgtcatca tgtattacgc gccgaaaatc
     5281 ttcgaactgg cgggttatac caacaccacc gagcaaatgt gggggacagt gattgtcggc
     5341 ctgaccaacg tacttgccac ctttatcgca atcggccttg ttgaccgctg gggacgtaaa
     5401 ccaacgctaa cgctgggctt cctggtgatg gctgctggca tgggcgtact cggtacaatg
     5461 atgcatatcg gtatccactc tccgtcggcg cagtatttcg ccatcgccat gctgctgatg
     5521 tttattgtcg gttttgccat gagtgccggt ccgctgattt gggtactgtg ctccgaaatt
     5581 cagccgctga aaggccgcga ttttggcatc acctgctcca ccgccaccaa ctggattgcc
     5641 aacatgatcg ttggcgcaac gttcctgacc atgctcaaca cgctgggtaa cgccaacacc
     5701 ttctgggtgt atgcggctct gaacgtactg tttatcctgc tgacattgtg gctggtaccg
     5761 gaaaccaaac acgtttcgct ggaacatatt gaacgtaatc tgatgaaagg tcgtaaactg
     5821 cgcgaaatcg gcgctcacga ttaatctcct caggcttcct cccgtcgcgg aggaagccgc
     5881 ctcttgcagt catcttttct tcgctctatc ctctgccgct atgaaaacat cccgtctccc
     5941 tatagccatc caacaggccg ttatgcgccg cctgcgggaa aaactcgccc aggccaacct
     6001 gaagctaggg cgtaactacc cggagccaaa actctcttac acccagcgcg gaacctccgc
     6061 cggaacagcc tggctggaaa gctatgaaat ccgcctcaat cccgttttgc tgttggaaaa
     6121 cagtgaagct tttattgaag aagtggtacc gcacgaactg gcacatttgc tggcatggaa
     6181 acatttcggc cgcgtagcgc cacatggcaa agagtggaag tggatgatgg aaagcgtgct
     6241 gggcgttccc gcccgtcgta cgcatcagtt cgaactgcaa tccgtgcgtc gcaacacctt
     6301 cccctaccgc tgtaagtgcc aggaacatca gcttaccgta cgccgccata atcgcgtagt
     6361 tcgtggcgag gccatctatc gctgtgttca ctgcggtgaa cagctggttg cgaaataacc
     6421 acctgaacta tcaggaactt tcctgatctg gctgattgca taccaaaaca actttcgcta
     6481 cgttgctggc tcgttttaac acggagtaag tgatgtaccg ttatttgtct attgctgcgg
     6541 tggtactgag cgcagcattt tccggcccga cgttggccga aggtatcaat agtttttctc
     6601 aggcgaaagc cgcggcggta aaagtccacg ctgatgcgcc cggtacgttt tattgcggat
     6661 gtaaaattaa ctggcagggc aaaaaaggcg ttgttgatct gcaatcgtgc ggctatcagg
     6721 tgcgcaaaaa tgaaaaccgc gccagccgcg tagagtggga acacgtcgtt cccgcctggc
     6781 aattcggtca ccagcgccag tgctggcagg acggtggacg taaaaactgc gctaaagatc
     6841 cggtctatcg caagatggaa agcgatatgc ataacctgca gccgtcagtc ggcgaggtga
     6901 atggcgatcg cggcaatttt atgtacagcc agtggaatgg cggcgaaggc cagtacggtc
     6961 aatgcgccat gaaggtcgat ttcaaagaaa aagctgccga accaccagcg cgtgcacgcg
     7021 gtgccattgc gcgcacctac ttctatatgc gcgaccaata caacctgaca ctctctcgcc
     7081 agcaaacgca gctgttcaac gcatggaaca agatgtatcc ggttaccgac tgggagtgcg
     7141 agcgcgatga acgcatcgcg aaggtgcagg gcaatcataa cccgtatgtg caacgcgctt
     7201 gccaggcgcg aaagagctaa cctacactag cgggattctt tttgttaacc cctaccccac
     7261 gcgtacaacc gcgtggggag acgacgcgga tttttaacta tgcgtatccc ccgcatttat
     7321 catcctgaac cactgaccag ccattctcac atcgcgcttt gcgaagatgc cgccaaccat
     7381 atcgggcgcg tactgcgcat ggggccgggg caggcgttgc aattgtttga cggtagcaac
     7441 caggtctttg acgccgaaat taccagcgcc agcaaaaaaa gcgtggaagt gaaggtgctg
     7501 gaaggccaga tcgacgatcg cgaatctccg ctgcatattc acctcggtca ggtgatgtcg
     7561 cgtggtgaaa aaatggaatt tactatccag aaatcgatcg aactcggtgt aagcctcatt
     7621 acgccacttt tttctgagcg ctgcggcgtt aaactggata gtgaacgtct gaacaagaag
     7681 cttcagcagt ggcagaagat tgcaattgct gcctgtgagc agtgtggtcg taaccgggtg
     7741 ccggaaatcc gtccagcgat ggatctggaa gcctggtgtg cagagcagga tgaaggactg
     7801 aaactgaatc ttcacccgcg cgccagtaac agcatcaata cgttgccttt accggttgaa
     7861 cgcgtccgcc tgctgattgg cccggaaggc ggtttatcgg cagatgaaat tgccatgact
     7921 gcccgctatc aatttactga tatcctgttg ggacctcgcg ttttgcgtac agagacaact
     7981 gcgctcaccg ccattaccgc gctacaagta cgatttggcg atttgggcta acggagaaga
     8041 ataatgatca agctcggcat cgtgatggac cccatcgcaa acatcaacat caagaaagat
     8101 tccagtttcg ctatgttgct ggaagcacag cgtcgtggtt acgaacttca ctatatggag
     8161 atgggcgatc tgtatctgat caatggcgaa gcccgcgccc atacccgcac gctgaacgtg
     8221 aagcagaact acgaagagtg gttttcgttc gtcggtgaac aggatctgcc gctggccgat
     8281 ctcgatgtga tcctgatgcg taaagacccg ccgtttgata ccgagtttat ctacgcgacc
     8341 tatattctgg aacgtgccga agagaaaggg acgctgatcg ttaacaagcc gcagagcctg
     8401 cgcgactgta acgagaaact gtttaccgcc tggttctctg acttaacgcc agaaacgctg
     8461 gttacgcgca ataaagcgca gctaaaagcg ttctgggaga aacacagcga catcattctt
     8521 aagccgctgg acggtatggg cggcgcgtcg attttccgcg tgaaagaagg cgatccaaac
     8581 ctcggcgtga ttgccgaaac cctgactgag catggcactc gctactgcat ggcgcaaaat
     8641 tacctgccag ccattaaaga tggcgacaaa cgcgtgctgg tggtggatgg cgagccggta
     8701 ccgtactgcc tggcgcgtat tccgcagggg ggcgaaaccc gtggcaatct ggctgccggt
     8761 ggtcgcggtg aacctcgtcc gctgacggaa agtgactgga aaatcgcccg tcagatcggg
     8821 ccgacgctga aagaaaaagg gctgattttt gttggtctgg atatcatcgg cgaccgtctg
     8881 actgaaatta acgtcaccag cccaacctgt attcgtgaga ttgaagcaga gtttccggtg
     8941 tcgatcaccg gaatgttaat ggatgccatc gaagcacgtt tacagcagca gtaacccacc
     9001 ttagcgagaa ggatctcgtt gagactctga gtgacagcgc ccttctttcc acgcatactg
     9061 gccgctgttg cttttttgaa ccaggaaaca gaacctctga caatgaattt acagcatcac
     9121 tttcttattg ccatgcctgc tctccaggat ccgattttcc gtcgttccgt ggtctacatt
     9181 tgcgaacata ataccaatgg tgcaatgggg atcatcgtca acaagccgct ggaaaatctc
     9241 aaaattgaag ggattctgga aaagctgaag atcacgccgg agccgcgtga tgaatcaatc
     9301 cgtctggata aaccggttat gctcggcggc ccactggctg aagatcgcgg gtttattttg
     9361 catactccgc cctccaattt tgcttccagc attcgcattt cagacaacac ggtgatgacc
     9421 acttcccgcg atgtgctgga aacgctcggc accgataaac aaccgtctga cgtattggtg
     9481 gctctgggct atgcctcctg ggaaaaaggt caactggaac aagaaattct cgataacgcg
     9541 tggctaacgg ccccggcgga tctgaatatt ctgttcaaaa cgccgattgc cgaccgctgg
     9601 cgcgaggcgg caaaactgat tggtgtggat attctcacca tgcctggtgt ggcaggacac
     9661 gcctgatgag tggaacctta ctcgccttcg acttcggcac caaaagcatt ggcgtagcgg
     9721 tcggccaacg cattaccggc accgctcgcc ctttgcctgc aattaaagcg caggacggta
     9781 cgccggactg gaacctcatc gaacgtttac tgaaagagtg gcagcctgac gaaatcatcg
     9841 tcggtttgcc gctgaatatg gacggcaccg aacagccgtt aaccgccaga gcgcgcaaat
     9901 ttgccaaccg tattcatggt cgtttcggtg ttgaagtaaa gctccatgac gagcgtctta
     9961 gcactgtgga agcccgttcc ggtctttttg aacagggcgg ctatcgggcg ctcaacaaag
    10021 gcaaagttga ctctgcctct gcggttatta ttctcgaaag ctatttcgag cagggatatt
    10081 aaggcgatct aaaagcctgg cggatagtcg ttaccgccat cttgccggat gcggcgtgaa
    10141 cgccttatcc agcacacatc tggcagcggc tgtaggtctg ataagacgcg ttagcgtcgc
    10201 atcagacatt tattgccttt ataggcccta taaacttgcg catcgggcat ggcaacgtca
    10261 caaacgccct tcccccaccc gctgctgata actctgctga aacgttaaca tccccacctg
    10321 ctgcccggtt tgaataacat gcggtaactg gtgggttttc ccttcgcgaa tcaaattccc
    10381 caccgcggga gtgttaatca gtaattcaaa cagcgccacg cgtccttcct gtttatccac
    10441 ttccagcttt tgtgacagca ctgcccgtaa actacccgca agttgattac gtacgggatc
    10501 tttttcctgc gccggaaatg aatccaccag tcgctcaact gcctgcgcgg caccacgtgt
    10561 atgtaaggtt gccagcacca gatgcccggt ttctgccgcc gtcagcgcca gacggattgt
    10621 ctcactgtca cgtagctctc cgagcaaaat cacatcagga tcttcccgca atgcagcccg
    10681 caaccccgat gcgaacgtca tacagtgcaa accaatttcc cgctgctgga tcaaacatcg
    10741 ctggctggca tagagatatt ccacaggatc ttccagcgtc agaatatgcg catcggcatg
    10801 ttgattaaga tagccaacca tcgccgccag cgtggtagat ttgccactcc ccgtagcccc
    10861 cgtcaccaga atcaggccat tctcgctctt gagtaattcc ggcaataccg gtggcgcacc
    10921 aagctgttcg agctgtggac agtgcgaagg taacaaccgt aatgccagag aaataccttg
    10981 ccgttgcgcg aacgcactgc cacgcaaccg ctggttttcc gccagcgaca cggcaaaatc
    11041 cagctggcca ttttccagca atattgcccg ctgatcgtca tccagccact cccgcagtag
    11101 ctcttcgacg tccggcgcat caaacggcgc agcttccatt ctgccacgaa tgcgccatcg
    11161 tgcgggccag gcgctgcaca ggtgtagatc cgagacgtta tgctttacac taagggccac
    11221 aatttcttcc atattcatac taagatcctc ggaaaatgaa cgatattgcg cataaccggg
    11281 cacaggtccg ggacaaaatc tcagcggctg caacgcgttg cggccgttct ccagaagaaa
    11341 ttacgctgct tgcagtcagt aaaacaaaac ctgcgagcgc catcgcagaa gccattgatg
    11401 ccgggcagcg tcaatttggt gaaaactacg ttcaggaagg ggtagataaa attcgccact
    11461 ttcaggaact gggcgtaaca ggattagaat ggcattttat tggcccgttg cagtctaata
    11521 aaagccgcct agtggcagag catttcgact ggtgtcatac catcgaccgt ttgcgcattg
    11581 ctacccgcct caacgatcag cgcccggcag aacttccccc tcttaacgta ctgattcaaa
    11641 ttaatattag tgatgaaaac agtaagtccg ggattcaact ggcagaactg gacgagctgg
    11701 cagcggcggt cgctgaacta ccgcgtttac gtctgcgcgg gctgatggca atccctgcgc
    11761 ctgagtcaga atatataagg cagtttgaag ttgcacgcca aatggctgta gcatttgccg
    11821 gactgaaaac gcgctacccg catatcgaca cgctctctct gggaatgtcg gacgatatgg
    11881 aagccgccat tgcggcaggt agcacgatgg ttcgtatcgg cactgcaatt tttggtgcgc
    11941 gtgattactc taaaaaataa ggaattaaag gaacgccatg aatacgttga ctttcctgct
    12001 ttcaacggtc attgagctgt ataccatggt gctgttatta cgcatctgga tgcagtgggc
    12061 tcattgtgat ttttacaacc ccttctcaca gtttgtagtg aaggtagcgc agccaattat
    12121 cgggccactg cgccgcgtta ttccggcaat gggtcccatt gacagcgcct cgctgctggt
    12181 tgcctatatt ctcagtttta tcaaagccat tgtgctgttt gaagtggtga ccttcctgcc
    12241 aatcatctgg attgccggtt tactgattct gctgaaaacc atcggcttgc tgattttctg
    12301 ggtcctgctg gtgatggcga ttatgagctg ggtaagccag ggtcgtagcc cgattgaata
    12361 cgtgctgatt cagctggccg atccgctgct gcgcccgatt cgccgcttgc taccggcaat
    12421 gggtgggatt gatttctcgc cgatgatcct cgttctgctg ctgtatgtca tcaatatggg
    12481 tgtcgcagaa gtattacagg caaccggaaa catgctgctg ccggggctgt ggatggcgtt
    12541 atgagtgccg taacagttaa tgatgacggt ctggttttac ggctctatat tcagccgaaa
    12601 gccagccgtg attctattgt cggtttacat ggcgacgaag ttaaagtcgc cattaccgcg
    12661 ccgccggttg acggccaggc caacagtcat ctggtgaagt ttctcggtaa gcaattccgg
    12721 gttgccaaaa gccaggtggt gattgaaaaa ggcgaacttg gccgccacaa acaaattaaa
    12781 atcattaatc cgcaacaaat cccgccagaa atcgcggcgt taattaatta ggtatcctat
    12841 gcaaaaagtt gtcctcgcaa ccggcaatgc cggtaaagtg cgtgagctgg cgtcgctgct
    12901 tagcgacttt ggtcttgata tcgtggccca aacagacctc ggcgttgatt ccgctgaaga
    12961 aaccggccta acctttatcg aaaacgcgat tctgaaagcg cgccatgcgg caaaagtgac
    13021 cggtttaccg gcaattgccg acgactctgg tctggcggta gatgcgctgg gtggcgcgcc
    13081 gggtatttac tccgcgcgtt attccggtga agacgcgacc gatcaaaaga atctgcaaaa
    13141 actgttggaa acactgaaag acgtaccgga cgaccaacgt caggcgcgtt tccactgcgt
    13201 gctggtatat ctgcgtcacg cggaagatcc gactccgctg gtatgccacg gcagctggcc
    13261 gggcgtgatt actcgcgaac cagcgggcac tggtggcttt ggttatgatc caatcttctt
    13321 cgtaccttcc gaagggaaaa ccgccgccga aatgacccgc gaagaaaaga gcgccatttc
    13381 ccaccgtggt caggcgttga aactgctgct ggacgcttta cgtaatggtt aaattaccgc
    13441 cgctgagtct ctacattcac atcccgtggt gcgtgcagaa atgcccgtac tgcgatttca
    13501 actctcacgc gttgaaagga gaagtgccgc acgacgatta tgttcagcat ctgctttgcg
    13561 atctggacaa tgatgtggct tacgctcagg gccgtgaagt aaagacaatc tttattggcg
    13621 gtggtacgcc gagcctgctt tccggcccgg cgatgcaaac gctgctagac ggcgtgcgtt
    13681 cgcgtttgcc gctggcagcg gatgcagaaa ttactatgga agcgaaccca ggcacggtag
    13741 aagcggatcg ctttgtcgat tatcagcgtg ctggtgtaaa ccgcatctct attggtatgc
    13801 agagttttag cgaagaaaag ctgaaacgac ttgggcgtat tcatggcccg caagaagcga
    13861 aacgagcggc aaagctggcg agcggtttag ggttacgtag ctttaacctt gatttgatgc
    13921 atggactacc ggatcaatca ctggaagagg cgcttggcga tctacgccag gccattgaac
    13981 tgaatccgcc gcatctttcc tggtatcaac tgaccatcga acctaatacg ctgtttggct
    14041 cgcgaccgcc ggtgctgccg gacgatgacg cgctgtggga tattttcgaa caggggcatc
    14101 agttattaac cgcagcgggt tatcagcaat atgaaacttc cgcttacgcc aaacccggtt
    14161 atcagtgcca gcacaatctc aactactggc gctttggtga ctacatcggt attggctgcg
    14221 gcgcgcacgg caaagtgacc ttcccggatg ggcgcattct gcgtaccact aaaacgcgtc
    14281 atcctcgtgg ttttatgcaa ggaaggtatc tggaaagcca gcgtgatgtc gaagccgcag
    14341 ataagccgtt tgagttcttt atgaatcgct tccgtctgct ggaggccgcg ccgcgcgtgg
    14401 agtttattgc gtataccggg ctttgcgaag atgtgattcg cccacagtta gacgaggcga
    14461 ttgcccaggg ttatctcacc gaatgtgcgg attactggca gataacggaa catggtaaat
    14521 tattcttaaa ctcacttctt gaattattca tcgctgagta atcattaatg cgcctgcggg
    14581 cgcattaatc acttctttta tccccccgtt aattaacctc ggcagaaata tatttcttac
    14641 tgaataaata tcgacgacat ttattatata tatttagtga atatttacta atcattcatt
    14701 gattataaat atgtacaacc tggcgcacaa atttaatatt taatttctga ctttatttca
    14761 agttgtgata aacatagaca agttgtcaga caggttaacc aatttgtcgc acaaagcact
    14821 gataagcaca attagatatc aaccaatttc catccttcaa cagggagttt ttttatgaaa
    14881 atgaggtcat ttacccgctc attagtctgc gcatccttgc tggctctggt tagcactggc
    14941 gtaaacgccg cagaaaaagt gacgttaaag ttagcacaca acctggaaag aagtcatgtt
    15001 gtacatcaga gttttgaaga gctggcgaaa gaggtaaaac agctgtcaaa aggtaatatg
    15061 gtgatccgta tttatcccag cagccagatg ggtaatgcgc gagaaactat ggaattgttg
    15121 caaaatggcg cactggatat gaccaaaggt tccgccagtg atctggagtc ttttgataat
    15181 atttatgcta tctataattt gccgttctta ttaagttgtt tttggtgaag ttggtaaaga
    15241 aattatggac tcaacaaaag gcaaaggctt ttttgcttta tcagcttatg tagcgggtac
    15301 tcgcagtttc tatgcgaaaa aaccgattac aaagccagaa gatcttaaag gactgaaaat
    15361 tcgtgttcag ccaagtccga ccaccatcaa aatgattgaa ctgatgggtg gttcacccac
    15421 tccaatttca tttggcgaag tttatactgc gatgcagcaa ggtgtagtag atggcgcaga
    15481 aaataacgtg ccttcctgga tgcagactcg acatattgaa attgccaaag ttttttctga
    15541 agatgaacac gcctcaattc ctgacttttt ggttatctcc accaaaacat ggaataaatt
    15601 acccccagaa cagcaacaaa tcctcgaaac agcggcgaag aaatccgaag cttatcaaca
    15661 aaaattgtgg gaaaaaattg acgctgacac acgtgctcag gcgaaagcta tggggggtga
    15721 aattgtaaaa gtggataaag cgccattccg taaagccgtg caacctctgt ttgacgactt
    15781 caaaaaagat ccaaaacaag ctgctttgtt agagaaattc gacaacgctg ctcaataata
    15841 ttccgggcca gcctctggcc ctgattttcc aggatgctta caatgatatt taaccgcctg
    15901 aaacttgcgg tagaccgcgt cattgccgcg ttttctgtcg ccgtaatgct tgctctggtt
    15961 gtatgcgtag tctggcaagt atttagccgc tacgtactta accagccaag tacattaacc
    16021 gatgagctgg cacgcttttt gatgatctgg gtaggattat taggtgctgc atataccgtc
    16081 ggcgcgcagc gacatctctc tattgattta tttgctctcg ccctgaataa gcgcaaacaa
    16141 ttacttctta gtatagtcat taacgttctc attttaggtt ttgccggttc ggtcattgtg
    16201 actggtggcc tgaaattaat tgataaaaca ctggcaacat cacaggtatc tgccgctatg
    16261 caaataccga tgggatacgt atatatcatt ctgccattaa gtggagtggt aatgatgttt
    16321 tatgc
//
LOCUS       DAJTYG010000081        16032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTYG010000081 DAJTYG010000000
VERSION     DAJTYG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.081"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO568_002884"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO568_002884"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR6642167.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="OO568_002885"
     CDS             1020..1703
                     /locus_tag="OO568_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR6642168.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="OO568_002886"
     CDS             1700..2161
                     /locus_tag="OO568_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR6642169.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="OO568_002887"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="OO568_002887"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6642170.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHLESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIL
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="OO568_002888"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="OO568_002888"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR6642171.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSRAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSAFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO568_002889"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO568_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR6642172.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO568_002890"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO568_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR6642173.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            5380..6210
                     /locus_tag="OO568_002891"
     CDS             5380..6210
                     /locus_tag="OO568_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6642174.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="OO568_002892"
     CDS             complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="OO568_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6642175.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(7339..8799)
                     /locus_tag="OO568_002893"
     CDS             complement(7339..8799)
                     /locus_tag="OO568_002893"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6642176.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGSAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="OO568_002894"
     CDS             complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="OO568_002894"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6642177.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            10404..11747
                     /gene="gntP"
                     /locus_tag="OO568_002895"
     CDS             10404..11747
                     /gene="gntP"
                     /locus_tag="OO568_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6642178.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="OO568_002896"
                     /pseudo
     CDS             complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="OO568_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
     gene            complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="OO568_002897"
     CDS             complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="OO568_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR6642179.1"
                     /translation="MKWCKRGYVLAAMLALASATIQPADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(13436..13966)
                     /gene="fimF"
                     /locus_tag="OO568_002898"
     CDS             complement(13436..13966)
                     /gene="fimF"
                     /locus_tag="OO568_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR6642180.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(13976..>16032)
                     /gene="fimD"
                     /locus_tag="OO568_002899"
     CDS             complement(13976..>16032)
                     /gene="fimD"
                     /locus_tag="OO568_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR6642181.1"
                     /translation="SVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSG
                     SKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRG
                     FAPVIHGIAHGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGS
                     TQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYG
                     GTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNES
                     GTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGK
                     LQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAW
                     QKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLED
                     NNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVL
                     AHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVAL
                     DTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSE
                     SSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAE
                     CR"
BASE COUNT         3866 a         4130 c         3923 g         4113 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagcc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tgactgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct ttttttccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc tggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattct
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccgg gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgaatccg tgcactggaa caggcgattg gagttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggg gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagcg
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtaag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaac cgctagtata ttctgaatat tccgaatagt tacgtagatg
     4981 aaaatacttg tcaccgctgc tacttaattc cggtgggaaa ttatttattt ccgcataatc
     5041 agctatgtat tatttgaaac aattagtttg taaataatat aaattgataa catatcaaat
     5101 acatcaaaaa taccattcac aggcattatt aatatagacc tcaatatagc aacatcaaat
     5161 tccaattagt aataagatat gcttttaaat tgcatgcaaa taattttaaa tgagcgtttt
     5221 ttgatacttt agagtgtttc ctacccttta atatcactgt gtagagttga tatcactaca
     5281 acagaaacat gcatgtcatt tgtttacttc tctttttact acagaaaaat ttatctattt
     5341 ttctaaacat cagttaaata ctcataaaag gtaactatta tgtctatgcc attaagcaat
     5401 gccgctaata accaattcaa tacaaataat cacttccttc atcatccaaa agtcgacagt
     5461 gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta tcttctgcct
     5521 cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt
     5581 aatattggaa aacagtcctg gccatttaat tatgtgacag atacatgcag agaattcaat
     5641 ggtattacaa caacaggacg tatgctctat cgaaacttga aaatcacctc agctttagac
     5701 gaaatatatg gtggtatatg taaaaaggcg catgctgcta cagagttagc agaaggttta
     5761 cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac agttcatgaa
     5821 ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat aggatatatt
     5881 tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag aattgatatc
     5941 tatattgatc agataataac tgtctcaggg gtagcaaatt cttccggatt cgcccttgcc
     6001 gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat agaagcctat
     6061 cctggtaccg cggagatcca ttcaaaaatg ggatataacg ttatccccgg tgatgaagat
     6121 gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa
     6181 aatggtgaat ggaattatat tggtaaatag ccactgagaa gaatgagtac taatattcag
     6241 gttatacaga tatttaaaca aaaccctgca atttacaaag taatactcat tgcgacttct
     6301 taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag tcaggcgtcg
     6361 actttatcca gcggccatga atcaaagaga tagccgtcaa aataaatatc gtcaacgttc
     6421 gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc
     6481 gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct
     6541 ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg attgttttcc
     6601 cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt tgcttcagca
     6661 atggcgagat ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct ggaagccagt
     6721 tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc
     6781 agtgcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa cagctcaaaa
     6841 ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct gcctgagcta
     6901 tcaagcacat agataccggc accccggcgc acttccacca gccctttgat ctccagcatg
     6961 atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc
     7021 ggcggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgcg gatcatcgcc
     7081 ccgacttcct ggtaaggtct ttgcgcagag gtgacagatt tcatggtgtt gtgtccgtta
     7141 ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg
     7201 gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag ttggcacgta
     7261 caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga ccagtaagtc
     7321 tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcgcacag
     7381 ctgctgatat gccgccgtca ccgcgccaac aaagtcggca ttctgcggca gatcatcggc
     7441 aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt
     7501 aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcactac cctgctcatc
     7561 cacgccctgg gtgtaacgca tccagccagc cacgcccagc gccaggtgac gccagctgcc
     7621 gccgttttgc aggtgcagac gcaccgggtc cagtagacgc tgcggtaact tctggctgcc
     7681 gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag
     7741 cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc
     7801 ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat
     7861 ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc cgttcagcat
     7921 acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgcgc ccactttatc
     7981 ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga acggttcgca
     8041 ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta aggtttctgg
     8101 cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat
     8161 ccatgccgct agctgcgggt cacgcgcctg agccagcccc agtaccgcga ccttcgccac
     8221 atgaccgttt tcacgcacgt tatcgcagga catcaccgta aatgctttca gccctttttc
     8281 acgacgcaga cgcaaggctt cgacgatgta accaatcgcg gacttcggcg cagtcgggtt
     8341 ttccagatcg tgcttgatca gcgggttatt gagatccagt tgaccgcttg ccgcatcagc
     8401 gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac gcgccatcgc
     8461 gttgagaata ccttcgcagc cgtcgatttc cggatgcagc gcttctttca tcgaaccgat
     8521 aattttcagc tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca gttgctgttt
     8581 tttcaggttt tcgatcagca tgcggtcgtt gcctggcatc aggttaactt cgcagatgcc
     8641 ccagtcgctg tcggtgcttt ccagcagatg atgggtatac agcgcctggt gcgcgcggtg
     8701 aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat cccatgacgg
     8761 gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca gaatccgtct
     8821 tcagagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc cggtgggtat
     8881 taacggctaa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc ggccaggcct
     8941 ttcagacgac caattgcgga gtaaccaggg ttggttttct tcttcaggtc gtccagcatc
     9001 tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc tttacgacgg
     9061 tgttcttctt caacaatcgc tttcaccact tcgtacatat caacgtcacc gttcaggtgc
     9121 gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag atgggtgaag
     9181 taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc acgcacgccg
     9241 taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc aaccatccac
     9301 tgcatatctt caatggtgga aacaatgcgc ggcaagccga ggatcgggcg cggcggatcg
     9361 tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat cgctttcagg
     9421 aagacggcaa agttttcgcg cagtttggct ttgtcgatat ctttgtacag ctccaggtgc
     9481 ttacggaact ggtcgagggt atacccttct tccgcgcccg gcagaccggc aatgatgtta
     9541 cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc tgcctgagca
     9601 atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg catttcgaat
     9661 gcagcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag cacgtattcg
     9721 aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt gcgaatgcca
     9781 cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgttc atagttgcca
     9841 gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca cactaggcct
     9901 gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac ttcgccgttc
     9961 gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac atcagctaaa
    10021 gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt tcctctttca
    10081 catagtgggt tccacagcaa agagacgcgg aaccttggag ttgtttaggt atccggatga
    10141 acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt tctacctttt
    10201 ggtaagacat gtcaacccga actctgaaat tggttaacca catcacaaga atttcactct
    10261 catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc agaggatgtg
    10321 acattcatcg caacaatggt tgaccaattt gcataacatg tcgcccaaaa taacaacggt
    10381 tcaaccaagt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg gtattcggca
    10441 ttggcctgat gctgatactg aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg
    10501 tggcggcgct gtccgtcggg atgctggcag gcatggattt gatgtcgctg ctgcacacca
    10561 tgaaagcggg cttcggcaac acgctgggag aactggctat catcgtggtg ttcggtgcgg
    10621 tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcacat acgctgctgg
    10681 cgcgtctagg tctgcgctat gttcagctgt cggtgattat catcggcctt attttcggtc
    10741 tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt gttattgccg
    10801 ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct gccactaccg
    10861 cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct tatggcgcgg
    10921 atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta atctgcgcag
    10981 gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag
    11041 cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt ctggtgccgc
    11101 tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg gtaaaagata
    11161 cccctgcctg ggaagtggtt aactttattg gttcctcgcc gattgcgatg tttattgcga
    11221 tggtggttgc attcgtactc tttggcaccg cgcgtggtca tgacatgcag tgggtgatga
    11281 acgcttttga aagcgcggtg aagagtattg caatggtgat tctgatcatc ggtgcgggtg
    11341 gcgtgctgaa gcagaccatc atcgacaccg gcattggcga caccatcggc atgttgatgt
    11401 cccacggcaa tatctcgccc tacattatgg catggctgat cactgtgcta attcgtctgg
    11461 cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt gctgcaatcc
    11521 ttgatccagc aaccggtcag ctggttggcg tgaatccggc gctgctggta ctggcgacgg
    11581 ctgcgggttc caacaccctc acccacatta atgatgcatc tttctggttg ttcaaaggtt
    11641 actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag ctggtcaact
    11701 ccgtggttgg gctgattatt gtgctgatta ttagcatggt agcgtaaaag aacaccagag
    11761 cctgccaatg gcaggctcag actgatgact aaagtctaaa aatgtgcccg gacagtcccc
    11821 tcgcccctcc ggggagaggg ttagggtgag gggaacagac cagtaaagtg cgctttgttt
    11881 atgccagatg cgacgctgac gcgtcttatc tggcctacaa agggctaacg tgcaggtttt
    11941 gtaggtctga tagcgtagcg cctcaggtac cagcattagc aatgcgctgt aattttttta
    12001 ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca cctgccctcc
    12061 ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagttccta ctgctcctaa
    12121 cgataccgtg ttattcgttg gaataatcgt accgttgcgc gtcaactgta cgccgacgcc
    12181 ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat ctgcggttgt
    12241 gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa gaggaattgg
    12301 cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa catcacaacc
    12361 gccagtgggc accaccacat cattattggc gtaaatattc cacacaaact gaaaatcatc
    12421 gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgaac cagctttaat
    12481 cgccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggct acggcttatc
    12541 cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg gatagctact
    12601 gccactatat tttacggtcc cggaaaaatt agataacacg ccgccgtaag ccgagcctcg
    12661 ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa agatttgcgt
    12721 cgaaagatct acgaccaggt tttgccccac attcacgaca ggcgcaaggt ttacataaac
    12781 attagcgctg ccaccgccaa tagggatagc ggtaccattg gcggttttac aggcgaatga
    12841 ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaatag ctcgtttcat
    12901 tacaattatc tcttcgggtt cagctgtagg tataggtgat gctaatcact gcctgaatgg
    12961 ttccctgagt ggctccgcca tttaccgtca atgctctgac ctgtaacggg aagtgcgctg
    13021 attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat gtgttgccac
    13081 tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta taatatccgg
    13141 tgctgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc ggacaattag
    13201 tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacata agactgaaag
    13261 aataaagatc gccgagatct accgtggcat tggtggtgga aaccgtacac ggtttggcga
    13321 cgaccttacc gttcaccgtg atggtgacat cggctggctg tatcgtcgca cttgcgagcg
    13381 ccaacattgc cgccaataca tacccacgtt tgcaccattt catgagcatc tccagttact
    13441 gatattcaag agtgaaggta gccgtagcat tgatatgccc cgcagtgaca ggcacctgtg
    13501 tcgccattag ccgggcgtaa aaattcagcg tatttggttt acccggcgtc agggtcgtcc
    13561 acgaaatcgc agacgatgga gcattaaggg gtatttgatt ttgctgctca ttcagaagct
    13621 gtattcccag tcccgcagcc gctgacaccg tattttcaag tgcaagcagg ttggcattgt
    13681 ggctatctgc aacgccggta aacccaactt ttacggcaga aacggcatta ccacagggtg
    13741 acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc aatgttgtta aattgcttcg
    13801 ccgcgttttc catcagatca acagtaaaat tggttgattc agcggccaca ctacagccgt
    13861 tatctctgac atagccgcgg atagtaatcg tgctatccgc agccaaaacg cgacttaccg
    13921 ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg cccccttaac
    13981 gacattcagc tgatagctgg gttaataact gctgctgact ctctggtggc agttgataat
    14041 tggcgacaca gtgagcattt tcctcttctc cccatttcac ctgaactttt cccgctaaag
    14101 gcattccgct gaggtaaacc tgaccattat ccgcaacaat gccgctactc tggctactct
    14161 ctgatgtcac catcgcccca aacggcagcg gcttattatt gtgagttagc gtcatgagca
    14221 gttttatccc aacgcgcgct ttaaactctg ctcgcacgat cgccccacga gtgggaacaa
    14281 cgttagcgac cgcgttatct aaatcgacgt tatcagccaa ggtattggta tccagcgcca
    14341 ctctattttc ccgatattca gtggcataag gcagcacggc ataaccacgc cagtcggtac
    14401 gcacccccgt ctggttttcg acttttgcat cttttgcgcc aggcgcttta acaagcacca
    14461 ccgtatcgtt taacggctgc cccagcgtta cgccattggc atgagccaat accccaccgc
    14521 tgactccgta atagagctgc ttaatatcat cgctatggct gtaaccgata ttggcattgc
    14581 cgtaaccacc gcgataattc agcgtggcgt agcctgtact tccgctatta ccatcgcctc
    14641 ccccggcata gccggtttgc acgctataac tgaggttgtt gtcttccagc aacgtaccgt
    14701 atacaccagc cagattggtc atccgaccgt tgagatcgtg tgacatgctg tagctggcac
    14761 tggcatgtcg ccactgagat ttactgtcag aacgcagcca gtggctgaaa ggaatattga
    14821 cgttaagcgc taacatctga tcccgtcctt tttgccaggc gtttttcgtc aggctatagc
    14881 tgagggtcca gttgatatct tcgaacgcag tatttaatcc agcctggaat tgctcatcga
    14941 cattactcgt tccccaataa gtttgatggc taccactcaa atacagtgtt gatgtgcgcc
    15001 cgagttgctg agtaacggtg agctgtaatt tcccgcgttt gttataagcg aggttgtaat
    15061 agtcggtgaa tttcggctta acctgaataa ctccatcctg tgtttcgatg ttgtagccat
    15121 tcattcgact gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa taacggtaac
    15181 ccactaactg aatattcgtg cctgattcat tgagcgattt gttatagaga aaacgcaccg
    15241 attgtccgtc atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc atatccacag
    15301 acagagcgcc cagtgcctcc atgtttttcc cgataccgaa attaaaagca cgataacgat
    15361 ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg agtaatgtac
    15421 tctggaaaaa gcggggtttt tcctgctgcg catttccact acggtattct cctgccgtaa
    15481 tggaataacg agtatgccct tcacgttgca aaagcgggac tgacgaatag ggtacggtaa
    15541 aaatctgcgt gctgccgtca gcctctttga tcgttacctg caagtcacca ctattacctg
    15601 cggcatagat atcattgatg gtaaaaggcc ccggtggcac cgtactatta taaatgccat
    15661 atccattttg tttaatagtg acctgtgcag taccatgagc aataccgtgg atcaccgggg
    15721 caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt gcgccgcgaa
    15781 agttaatacc atcgaaaata tcgccctggg tataaccatc acccaacgtc agccgggaac
    15841 gtaaaggaat tatgtctcgc tcaatccagg taatgatatg ctgccattta tttttgctac
    15901 ctgatgaact gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa cgccacgcac
    15961 caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc ccaatccgat
    16021 tctgtacact at
//
LOCUS       DAJTYG010000082        16025 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTYG010000082 DAJTYG010000000
VERSION     DAJTYG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.082"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..521
                     /gene="tuf"
                     /locus_tag="OO568_002900"
     CDS             <1..521
                     /gene="tuf"
                     /locus_tag="OO568_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6642182.1"
                     /translation="GRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLL
                     DEGRAGENVGILLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFF
                     KGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGR
                     TVGAGVVAKVLG"
     gene            704..898
                     /gene="bfd"
                     /locus_tag="OO568_002901"
     CDS             704..898
                     /gene="bfd"
                     /locus_tag="OO568_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR6642183.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            971..1447
                     /gene="bfr"
                     /locus_tag="OO568_002902"
     CDS             971..1447
                     /gene="bfr"
                     /locus_tag="OO568_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR6642184.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1444..1911)
                     /locus_tag="OO568_002903"
     CDS             complement(1444..1911)
                     /locus_tag="OO568_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6642185.1"
                     /translation="MDTTLPFLILYACLSDLLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            2290..2601
                     /gene="rpsJ"
                     /locus_tag="OO568_002904"
     CDS             2290..2601
                     /gene="rpsJ"
                     /locus_tag="OO568_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6642186.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2634..3263
                     /gene="rplC"
                     /locus_tag="OO568_002905"
     CDS             2634..3263
                     /gene="rplC"
                     /locus_tag="OO568_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6642187.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3274..3879
                     /gene="rplD"
                     /locus_tag="OO568_002906"
     CDS             3274..3879
                     /gene="rplD"
                     /locus_tag="OO568_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6642188.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3876..4178
                     /gene="rplW"
                     /locus_tag="OO568_002907"
     CDS             3876..4178
                     /gene="rplW"
                     /locus_tag="OO568_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6642189.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4196..5017
                     /gene="rplB"
                     /locus_tag="OO568_002908"
     CDS             4196..5017
                     /gene="rplB"
                     /locus_tag="OO568_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6642190.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5034..5312
                     /gene="rpsS"
                     /locus_tag="OO568_002909"
     CDS             5034..5312
                     /gene="rpsS"
                     /locus_tag="OO568_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6642191.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5327..5659
                     /gene="rplV"
                     /locus_tag="OO568_002910"
     CDS             5327..5659
                     /gene="rplV"
                     /locus_tag="OO568_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6642192.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5677..6378
                     /gene="rpsC"
                     /locus_tag="OO568_002911"
     CDS             5677..6378
                     /gene="rpsC"
                     /locus_tag="OO568_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6642193.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6391..6801
                     /gene="rplP"
                     /locus_tag="OO568_002912"
     CDS             6391..6801
                     /gene="rplP"
                     /locus_tag="OO568_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6642194.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6801..6992
                     /gene="rpmC"
                     /locus_tag="OO568_002913"
     CDS             6801..6992
                     /gene="rpmC"
                     /locus_tag="OO568_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6642195.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6992..7246
                     /gene="rpsQ"
                     /locus_tag="OO568_002914"
     CDS             6992..7246
                     /gene="rpsQ"
                     /locus_tag="OO568_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6642196.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7411..7782
                     /gene="rplN"
                     /locus_tag="OO568_002915"
     CDS             7411..7782
                     /gene="rplN"
                     /locus_tag="OO568_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6642197.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7793..8107
                     /gene="rplX"
                     /locus_tag="OO568_002916"
     CDS             7793..8107
                     /gene="rplX"
                     /locus_tag="OO568_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6642198.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8122..8661
                     /gene="rplE"
                     /locus_tag="OO568_002917"
     CDS             8122..8661
                     /gene="rplE"
                     /locus_tag="OO568_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6642199.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8676..8981
                     /gene="rpsN"
                     /locus_tag="OO568_002918"
     CDS             8676..8981
                     /gene="rpsN"
                     /locus_tag="OO568_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6642200.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9015..9407
                     /gene="rpsH"
                     /locus_tag="OO568_002919"
     CDS             9015..9407
                     /gene="rpsH"
                     /locus_tag="OO568_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6642201.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9421..9954
                     /gene="rplF"
                     /locus_tag="OO568_002920"
     CDS             9421..9954
                     /gene="rplF"
                     /locus_tag="OO568_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6642202.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9964..10317
                     /gene="rplR"
                     /locus_tag="OO568_002921"
     CDS             9964..10317
                     /gene="rplR"
                     /locus_tag="OO568_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6642203.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10332..10835
                     /gene="rpsE"
                     /locus_tag="OO568_002922"
     CDS             10332..10835
                     /gene="rpsE"
                     /locus_tag="OO568_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6642204.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10839..11018
                     /gene="rpmD"
                     /locus_tag="OO568_002923"
     CDS             10839..11018
                     /gene="rpmD"
                     /locus_tag="OO568_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6642205.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11022..11456
                     /gene="rplO"
                     /locus_tag="OO568_002924"
     CDS             11022..11456
                     /gene="rplO"
                     /locus_tag="OO568_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6642206.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11464..12795
                     /gene="secY"
                     /locus_tag="OO568_002925"
     CDS             11464..12795
                     /gene="secY"
                     /locus_tag="OO568_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6642207.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12827..12943
                     /gene="rpmJ"
                     /locus_tag="OO568_002926"
     CDS             12827..12943
                     /gene="rpmJ"
                     /locus_tag="OO568_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6642208.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13090..13446
                     /gene="rpsM"
                     /locus_tag="OO568_002927"
     CDS             13090..13446
                     /gene="rpsM"
                     /locus_tag="OO568_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6642209.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13463..13852
                     /gene="rpsK"
                     /locus_tag="OO568_002928"
     CDS             13463..13852
                     /gene="rpsK"
                     /locus_tag="OO568_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6642210.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13886..14506
                     /gene="rpsD"
                     /locus_tag="OO568_002929"
     CDS             13886..14506
                     /gene="rpsD"
                     /locus_tag="OO568_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6642211.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14532..15521
                     /gene="rpoA"
                     /locus_tag="OO568_002930"
     CDS             14532..15521
                     /gene="rpoA"
                     /locus_tag="OO568_002930"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6642212.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15562..15945
                     /gene="rplQ"
                     /locus_tag="OO568_002931"
     CDS             15562..15945
                     /gene="rplQ"
                     /locus_tag="OO568_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6642213.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4123 a         3768 c         4276 g         3858 t
ORIGIN      
        1 ccggtcgtgg taccgttgtt accggtcgtg tagaacgcgg tatcatcaaa gttggtgaag
       61 aagttgaaat cgttggtatc aaagagactc agaagtctac ctgtactggc gttgaaatgt
      121 tccgcaaact gctggacgaa ggccgtgctg gtgagaacgt aggtattctg ctgcgtggta
      181 tcaaacgtga agaaatcgaa cgtggtcagg tactggctaa gccgggcacc atcaagccgc
      241 acaccaagtt cgaatctgaa gtgtacattc tgtccaaaga tgaaggcggc cgtcatactc
      301 cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg actggtacca
      361 tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa atggttgtta
      421 ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt gaaggcggcc
      481 gtaccgttgg cgcgggcgtt gttgctaaag ttctgggcta attacacgtt aattagtttt
      541 gaattgaaaa gggcgcttcg gcgccctttt tgctgcccgt aatttgagct cgaaatattt
      601 agtcgtaatt ttgctcgctg aactatggcg caaataaaac tgtgctattg taatcataac
      661 cattctcatt tacactttgt gcagaatttg aacgggagca atcatgtacg tttgtttgtg
      721 taatggtgta agtgacaaaa aaattcgcca ggcagtccgt cagtttcatc cgcaatcttt
      781 ccaacaattg cgtaagttta ttcctgtggg aaatcaatgc ggtaagtgca ttcgcgccgc
      841 gcgcgaagtg atgcaggacg agttgatgca gatgccagaa tttaaagaga ttgcctgaag
      901 cccacgctta ttttttgaca tcccgctagc cccatctacg ctctaatagt ggaagcggag
      961 ggactataaa atgaaaggtg atgttaaaat cataaattat ctcaataaac tattgggaaa
     1021 tgagcttgtc gcaatcaatc agtattttct ccatgcccgg atgtttaaaa actggggcct
     1081 gatgcgtctc aatgacgtcg aataccatga atccatcgat gagatgaaac acgctgataa
     1141 atatatcgag cgtattttgt ttttagaagg aatccccaac ttacaggatt taggcaaact
     1201 ggggattggt gaagatgtcg aggagatgct gcaatctgac ctgcgattag aactggaagg
     1261 cgcgaaggat ctgcgtgagg ctatcgcata tgccgacagc gttcatgact atgttagccg
     1321 cgatatgatg atcgagatcc ttgctgatga agaaggtcat atcgactggc tcgaaactga
     1381 actggatctc atcggtaaaa tcggcctaca aaattacctg caatcacaaa ttaaagttaa
     1441 agattaacgg cctatcagca aactatccca gcctataacg aaacccgcag ccgccagaaa
     1501 tggcccaaaa ggcagcgggt tttttaatga ttcttttcct ctcatgagca gaccaataac
     1561 tacggctccg caagcgaatg aggcagcaag gaaaaccagt cgtggtaaga aggcccatga
     1621 atgccaggcg ccaagggcgg cgagaaactt cacatcacca tagcctaatc cttctttatg
     1681 tcgcagtatg cgataccccc agtaaatgac agcgaatgtg ccgtaaccaa caattgcccc
     1741 ccataacgca tcggctaaac catcaggatg acaaacttga tagaacaaaa gacctgacca
     1801 gagtaacggg cacgtaaatc tgtcaggtaa caagccatgc tttgcatccc agaaaaaaag
     1861 taggtctgat aaacaagcgt acaaaattag aaagggaagg gtagtgtcca taaaacatcc
     1921 tctgtaattg gagtatcacc atcgaataac tccggatgct cagcagcaga gagagcgctg
     1981 atttgttaac ctgattccgg tgaaaaacgc tatcttcaca accttacaaa tttcgtggaa
     2041 tgcagctcgc aatctgcgcg ttgcgattaa tttctacccg atgcttgcgt caggctcaga
     2101 tttgcgtata atgcgcgggc ttgtcgtagt tgacagcagg ttcaatctga acctcagtta
     2161 gccgaatttg gctacctaac aatgctccca atcggggagc tacgtaagaa cggttacact
     2221 ctcccatcaa tcgtaatggg tctgaggagt aatcattttc gtttataaaa taattggagc
     2281 tctggtctca tgcagaacca aagaatccgt atccgcctga aagcgtttga tcatcgtctg
     2341 atcgatcaag caaccgcgga aatcgtcgag actgccaagc gcactggtgc gcaggtccgt
     2401 ggtccgatcc cgctgccgac acgcaaagag cgcttcactg ttctgatctc cccgcacgtc
     2461 aacaaagacg cgcgcgatca gtacgaaatc cgtactcact tgcgtctggt tgacatcgtt
     2521 gagccaaccg agaaaaccgt tgatgctctg atgcgtctgg atctggctgc cggtgtagac
     2581 gtgcagatca gcctgggtta atcaggtcat tgagcgattg agaggttgaa acaatgattg
     2641 gtttagtcgg taaaaaagtg ggtatgaccc gtatcttcac agaagacggc gtttctatcc
     2701 cagtaaccgt aatcgaagtt gaagcaaacc gcgttactca ggttaaagac ctggctaacg
     2761 atggctaccg tgctattcag gtgaccaccg gtgctaaaaa agctaaccgt gtgaccaagc
     2821 ctgaagctgg ccacttcgct aaagctggcg tagaagctgg ccgtggtctg tgggaattcc
     2881 gcctggctga aggcgaagag ttcactgtag gtcagagcat tagcgttgaa ctgtttgctg
     2941 acgttaaaaa agttgacgta actggcacct ctaaaggtaa aggtttcgca ggtaccgtta
     3001 agcgctggaa cttccgtacc caggacgcta ctcacggtaa ctccttgtct caccgcgttc
     3061 cgggttctat cggtcagaac cagactccgg gcaaagtgtt caaaggcaag aaaatggcag
     3121 gtcagatggg taacgaacgt gtaaccgttc agagccttga cgtagtacgc gttgacgctg
     3181 agcgcaacct gctgctggtt aaaggtgctg tcccgggtgc aaccggtagc gacctgatcg
     3241 ttaaaccagc tgtgaaggcg taaggagata gcaatggaat tagtattgaa agacgcgcag
     3301 agcgcgctga ctgtttccga aactaccttc ggtcgtgatt tcaacgaagc gctggttcac
     3361 caggttgttg ttgcttatgc agctggtgct cgtcagggta ctcgtgctca gaagactcgt
     3421 gctgaagtaa ctggttccgg taaaaaaccg tggcgccaga aaggcaccgg ccgtgcgcgt
     3481 tctggttcta tcaagagccc gatctggcgt tctggtggcg tgacctttgc tgctcgtccg
     3541 caggaccaca gtcaaaaagt taacaagaag atgtaccgcg gcgcgctgaa aagcatcctg
     3601 tccgaactgg tacgtcagga tcgtctgatc gttgtcgaga agttctctgt agaagcgccg
     3661 aaaactaagc tgctggcaca gaaactgaaa gacatggctc tggaagatgt gctgatcatc
     3721 accggtgagc tggacgaaaa cctgttcctg gctgcgcgca acctgcacaa ggttgacgta
     3781 cgcgatgcaa ctggtatcga cccggttagc ctgatcgcct tcgacaaagt cgtaatgact
     3841 gctgatgctg ttaagcaagt tgaggagatg ctggcatgat tcgtgaagaa cgtctgctga
     3901 aggtgctgcg tgcaccgcac gtttctgaaa aagcgtctac tgcgatggaa aaatccaaca
     3961 ccatcgtact caaagttgct aaagacgcga ccaaagcaga aatcaaagct gctgtgcaga
     4021 aactgtttga agtcgaagtc gaagtcgtta acaccctggt agttaaaggg aaagttaaac
     4081 gtcacggaca gcgtatcggt cgtcgtagcg actggaaaaa agcttacgtc accctgaaag
     4141 aaggccagaa tctggacttc gttggcggcg ctgagtaagt cggaggagta atacaatggc
     4201 agttgttaaa tgtaaaccga catctccggg tcgtcgccac gtagttaaag tggttaaccc
     4261 tgagctgcac aagggcaaac cttttgctcc gttgctggaa aaaaacagca aatccggtgg
     4321 tcgtaacaac aatggccgta tcaccactcg tcatatcggt ggtggccaca agcaggctta
     4381 ccgtattgtt gacttcaaac gcaacaaaga cggtatcccg gcagttgttg aacgtcttga
     4441 gtacgatccg aaccgttccg cgaacatcgc gctggttctg tacaaagacg gtgaacgccg
     4501 ttacatcctg gcccctaaag gcctgaaagc tggcgaccag attcagtctg gcgttgatgc
     4561 tgcaatcaaa ccaggtaaca ccctgccgat gcgcaacatc ccggttggtt ctactgttca
     4621 taacgtagaa atgaaaccag gtaaaggcgg tcagctggca cgttccgctg gtacttacgt
     4681 tcagatcgtt gctcgtgatg gtgcttatgt caccctgcgt ctgcgttctg gtgaaatgcg
     4741 taaagtagaa gcagactgcc gtgcaactct gggcgaagtt ggcaatgctg agcatatgct
     4801 gcgcgttctg ggtaaagcag gtgctgcacg ctggcgtggt gttcgtccga ccgttcgcgg
     4861 tactgcgatg aacccggtag accacccaca tggtggtggt gaaggtcgta actttggtaa
     4921 gcacccggta actccgtggg gcgttcagac caaaggtaag aagacccgca gcaacaagcg
     4981 tactgataaa ttcatcgtac gtcgccgtag caaataattt tagaggataa gccatgccac
     5041 gttctctcaa gaaaggtcct tttattgacc tgcacttgct gaagaaggta gagaaagcgg
     5101 tggaaagcgg agacaagaag cccctgcgca cttggtcccg tcgttcaacg atctttccta
     5161 acatgatcgg tttgaccatc gctgtccata atggtcgtca gcacgttccg gtatttgtaa
     5221 ccgacgaaat ggtcggtcac aaactgggtg aattcgcacc gactcgtact tatcgcggcc
     5281 acgctgctga taaaaaagcg aagaagaaat aaggtaggag gaagagatgg aaactattgc
     5341 taaacatcgc catgctcgtt cttctgctca gaaggttcgc cttgttgctg acctgattcg
     5401 cggtaagaaa gtgtcgcagg ctctggatat tttgacctac accaacaaga aagcggctgt
     5461 acaggtcaag aaagttctgg aatctgccat tgctaacgct gaacacaacg atggcgctga
     5521 cattgacgat ctgaaagtta cgaaaatttt cgtagacgaa ggcccgagca tgaagcgcat
     5581 tatgccgcgt gcaaaaggtc gtgcagatcg tatcctgaag cgcaccagcc acatcactgt
     5641 ggttgtgtcc gatcgctgag actctggaga ctagcaatgg gtcagaaagt acatcctaat
     5701 ggtattcgcc tgggtattgt aaaaccatgg aactctacct ggtttgcgaa caccaaagaa
     5761 ttcgctgaca acctggacag cgattttaaa gtacgtcagt acctgactaa ggaactggct
     5821 aaagcgtccg tatctcgtat cgttatcgag cgtccggcta agagcatccg tgtaaccatt
     5881 cacactgctc gcccgggtat cgttatcggt aagaaaggtg aagacgtaga aaaactgcgt
     5941 aaggtcgtag cggacatcgc tggcgttcct gcacagatca acatcgccga agttcgtaag
     6001 cctgaactgg acgcaaaact ggttgctgac agcatcactt ctcagctgga acgtcgcgtt
     6061 atgttccgtc gtgctatgaa gcgtgctgta cagaacgcaa tgcgtctggg cgctaaaggt
     6121 attaaagttg aagttagcgg ccgtctgggc ggcgcggaaa tcgcacgtac cgaatggtac
     6181 cgcgaaggtc gcgtaccgct gcacactctg cgtgctgaca tcgactacaa cacctctgaa
     6241 gcgcacacca cttacggtgt aatcggcgtt aaagtgtgga tcttcaaagg cgagatcctg
     6301 ggtggtatgg ctgctgttga acaaccggaa aaaccggctg ctcagcctaa aaagcagcag
     6361 cgtaaaggcc gtaaataagg agcgtcgctg atgttacaac caaagcgtac aaaattccgt
     6421 aaaatgcaca aaggccgtaa ccgcggtctg gcgcagggta cggatgttag cttcggcagc
     6481 ttcggtctga aagctgttgg ccgtggtcgt ctgactgccc gtcagatcga agcagcacgt
     6541 cgtgctatga cccgtgcagt taagcgtcaa ggtaagatct ggatccgtgt gttcccggac
     6601 aaaccgatca ctgaaaagcc gctggcagtg cgtatgggta aaggtaaagg taacgtggag
     6661 tattgggttg ccttgattca gccgggtaaa gtcctgtatg aaatggacgg tgttccggaa
     6721 gagctggccc gtgaagcatt caagctggca gcagcgaaac tgccgattaa aaccaccttt
     6781 gtaactaaga cggtgatgta atgaaagcaa aagagctgcg tgagaagagc attgaagagc
     6841 tgaacaccga gctgctgaac ctgctgcgtg agcagttcaa cctgcgtatg caggctgcaa
     6901 gtggccagct gcaacagtct cacctgttga agcaagtgcg tcgcgatgtc gcacgcgtta
     6961 agactttact gaacgagaag gcgggtgcgt aatgaccgat aaaatccgta ctctgcaagg
     7021 tcgcgttgtt agcgacaaaa tggagaaatc cattgttgtt gctatcgaac gttttgtgaa
     7081 acacccgatc tacggtaaat tcatcaagcg tacgaccaaa ctgcacgtac atgacgagaa
     7141 caacgaatgc ggtatcggtg acgtggttga aatccgcgaa tgccgtccgc tgtccaagac
     7201 taaatcctgg acgctggttc gcgttgtaga gaaagcggtt ctgtaataca gtacactctc
     7261 tcgatacgaa taaacggctc agaaatgagc cgtttatttt ttctacccat atccttgaag
     7321 cggtgttata atgccgcgcc ctcgatatgg ggatttttaa cgacctgatt ttcgggtctc
     7381 agtagtagtt gacattagcg gagcactaaa atgatccaag aacagactat gctgaacgtc
     7441 gccgacaact ccggtgcacg tcgcgtaatg tgtatcaagg ttctgggtgg ctcgcaccgt
     7501 cgctacgcag gcgtaggcga catcatcaag atcaccatca aagaagcaat tccgcgtggt
     7561 aaggtcaaaa aaggtgatgt gctgaaggcg gtagtggtgc gcaccaagaa gggtgttcgt
     7621 cgcccggacg gttctgtcat tcgcttcgat ggtaatgctt gtgttcttct gaacaacaac
     7681 agcgagcagc ctatcggtac gcgtattttt gggccggtaa ctcgtgagct tcgtagtgag
     7741 aagttcatga aaattatctc tctggcacca gaagtactct aaggagcgaa tcatggcagc
     7801 gaaaatccgt cgtgatgacg aagttatcgt gttaaccggt aaagataaag gtaaacgcgg
     7861 taaagttaag aatgtcctgt cttccggcaa ggtcattgtt gaaggtatca acctggttaa
     7921 gaaacatcag aagccggttc cggccctgaa ccaaccgggt ggcatcgttg aaaaagaagc
     7981 cgctattcag gtttccaacg tagcaatctt caatgcggca accggcaagg ctgaccgtgt
     8041 aggctttaga ttcgaagacg gcaaaaaagt ccgtttcttc aagtctaaca gcgaaactat
     8101 caagtaattt ggagtagtac gatggcgaaa ctgcatgatt actacaaaga cgaagtagtt
     8161 aaaaaactca tgactgagtt taactacaat tctgtcatgc aagtccctcg ggtcgagaag
     8221 atcaccctga acatgggtgt tggtgaagcg atcgctgaca aaaaactgct ggataacgca
     8281 gcagctgacc tggcagcaat ctccggtcaa aaaccgctga tcaccaaagc acgcaaatct
     8341 gttgcaggct tcaaaatccg tcagggctat ccgatcggct gtaaagtaac tctgcgtggc
     8401 gaacgcatgt gggagttctt tgagcgcctg atcactattg ctgtacctcg tatccgtgac
     8461 ttccgtggcc tgtccgctaa gtctttcgac ggtcgtggta actacagcat gggtgtccgt
     8521 gagcagatca tcttcccaga aatcgactac gataaagtcg accgcgttcg tggtttggat
     8581 attaccatta ccactactgc gaaatctgac gaagaaggcc gcgctctgct ggctgccttt
     8641 gacttcccgt tccgcaagta aggtagggtt actaaatggc taagcaatca atgaaagcac
     8701 gcgaagtaaa acgcgtagct ttagctgata aatacttcgc gaaacgcgct gaactgaaag
     8761 cgatcatctc tgatgtgaac gcttccgacg aagatcgttg gaacgctgtt ctcaagctgc
     8821 agactctgcc gcgtgattcc agcccgtctc gtcagcgtaa ccgctgccgt caaacaggtc
     8881 gtccgcatgg tttcctgcgg aagttcgggt tgagccgtat taaggtccgt gaagccgcta
     8941 tgcgcggtga aatcccgggt ctgaaaaagg ctagctggta attgtcacca attgaatcac
     9001 gggagtaaag acagatgagc atgcaagatc cgatcgcgga tatgctgacc cgtatccgta
     9061 acggtcaggc cgcgaacaaa gctgcggtca ccatgccttc ctccaagctg aaagtggcaa
     9121 tcgccaacgt gctgaaggaa gaaggtttta ttgaagattt taaagttgaa ggcgacacca
     9181 agcctgaact ggaacttact ctgaagtatt tccagggcaa agctgttgta gaaagcattc
     9241 agcgtgtcag ccgcccaggt ctgcgcatct ataaacgtaa agatgagctg ccgaaagtta
     9301 tggcgggtct gggtatcgca gttgtttcta cctctaaagg tgttatgact gatcgtgcag
     9361 cgcgccaggc tggtcttggt ggcgaaatta tctgctacgt agcctaaatc ggaggaaaaa
     9421 atgtctcgtg ttgctaaagc accggtcgtt gttcctgccg gcgttgacgt aaaaatcaac
     9481 ggtcaggtta ttacgatcaa aggtaaaaac ggcgagctga ctcgtactct caacgatgct
     9541 gttgaagtta aacatgcaga taataccctg accttcggtc cgcgtgatgg ttacgcagac
     9601 ggttgggcac aggctggtac cgcgcgtgcc ctgctgaact caatggttat cggtgttacc
     9661 gaaggcttca ctaagaagct gcagctggtt ggtgtaggtt accgtgcagc ggttaaaggc
     9721 aatgtgatta acctgtctct gggtttctct catcctgttg accatcagct gcctgcgggt
     9781 atcactgctg aatgtccgac tcagactgaa atcgtgctga aaggcgctga taagcaggtg
     9841 atcggccagg ttgcagcgga tctgcgcgcc taccgtcgtc ctgagcctta caaaggcaag
     9901 ggtgttcgtt acgccgacga agtcgtgcgt accaaagagg ctaagaagaa gtaaggtaac
     9961 actatggata agaaatctgc tcgtatccgt cgtgcgaccc gcgcacgccg caagctccag
    10021 gagctgggcg caactcgcct ggtggtacat cgtaccccgc gtcacattta cgcacaggta
    10081 attgcaccga acggttctga agttctggta gctgcttcta ctgtagaaaa agctatcgct
    10141 gaacaactga agtacaccgg taacaaagac gcggctgcag ctgtgggtaa agctgtcgct
    10201 gaacgcgctc tggaaaaagg catcaaagat gtatcctttg accgttccgg gttccaatat
    10261 catggtcgtg tccaggcact ggcagatgct gcccgtgaag ctggccttca gttctaaggt
    10321 agaggtgtaa gatggctcac atcgaaaaac aagctggcga actgcaggaa aagctgatcg
    10381 cggtaaaccg cgtatctaaa accgttaaag gtggtcgtat tttctccttc acagctctga
    10441 ctgtagttgg cgatggtaac ggtcgcgttg gttttggtta cggtaaagcg cgtgaagttc
    10501 cagcagcgat ccagaaagcg atggaaaaag cccgtcgcaa tatgattaac gtcgcgctga
    10561 ataacggcac tctacaacac cctgttaaag gtgttcacac gggttctcgc gtattcatgc
    10621 agccggcttc cgaaggtacc ggtatcatcg ccggtggtgc aatgcgcgcc gttctggaag
    10681 tcgctggggt tcataacgtt ctggctaaag catatggttc caccaacccg atcaacgtgg
    10741 ttcgtgcaac tattgatggc ctagaaaata tgaattctcc agaaatggtc gctgccaagc
    10801 gtggtaaatc cgttgaagaa attctgggga aataaaccat ggcaaagact attaaaatta
    10861 ctcaaacccg cagtgcaatc ggtcgtctgc cgaaacacaa ggcaacgctg cttggcttgg
    10921 gtctgcgtcg tattggtcac accgtagagc gcgaggatac tcctgctatt cgcggtatga
    10981 tcaacgcggt ttccttcatg gttaaagttg aggagtaaga gatgcgttta aatactctgt
    11041 ctccggccga aggctccaaa aaggcgggta aacgcctggg gcgtggtatc ggttctggcc
    11101 tcggtaaaac cggtggtcgt ggtcacaaag gtcagaagtc tcgttctggc ggtggcgtac
    11161 gtcgcggttt cgagggtggt cagatgcctc tgtaccgtcg tctgccgaaa ttcggcttca
    11221 cttctcgtaa agcagcgatt acagccgaag ttcgtctatc tgacctggct aaagtagaag
    11281 gcggtgttgt agacctgaac acgctgaaag cggctaacat tatcggtatc cagatcgagt
    11341 tcgcgaaagt gatcctggct ggcgaagtaa cgactccggt aactgttcgt ggcctgcgtg
    11401 ttactaaagg cgctcgtgct gctatcgaag ctgctggcgg taaaatcgag gaataagtag
    11461 cagatggcta aacaaccggg attagatttt caaagtgcca aaggtggctt aggcgagctg
    11521 aaacgcagac tgctgtttgt tatcggtgcg ctgattgtgt tccgtattgg ctcttttatt
    11581 ccgatccctg gtattgatgc cgctgtactt gccaaactgc ttgagcaaca gcgaggcacc
    11641 atcattgaga tgtttaacat gttctctggt ggtgctctca gccgtgcttc tatctttgct
    11701 ctggggatca tgccgtatat ttcggcgtcg atcattatcc agctgctgac ggtggttcac
    11761 ccaacgttgg cagaaattaa gaaagaaggg gagtctggtc gtcgtaagat cagccagtac
    11821 acccgttacg gtactctggt gctggcaata ttccagtcga tcggtattgc taccggtctg
    11881 ccgaatatgc ctggtatgca aggcctggtg attaacccgg gctttgcatt ctacttcacc
    11941 gctgttgtaa gtctggtcac aggaacgatg ttcctgatgt ggttgggcga acagatcact
    12001 gaacgaggta tcggcaacgg tatttcaatc attatcttcg ccggtattgt cgcgggactc
    12061 ccgccagcca ttgcccatac tatcgagcaa gcgcgtcaag gcgacctgca cttcctcgtg
    12121 ttgctgttgg ttgcagtatt agtatttgca gtgacgttct ttgttgtgtt tgttgagcgt
    12181 ggtcaacgcc gcattgtggt aaactacgcg aaacgtcagc aaggtcgtcg tgtctatgct
    12241 gcacagagca cacatttacc gctgaaagtg aatatggcgg gggtaatccc ggcaatcttc
    12301 gcttccagta ttattctgtt cccggcgacc atcgcgtcat ggttcggggg cggtactggt
    12361 tggaactggc tgacaacaat ttcgctgtat ttgcagcctg ggcaaccgct ttatgtgtta
    12421 ctctatgcgt ctgcaatcat cttcttctgt ttcttctaca cggcgttggt tttcaacccg
    12481 cgtgaaacag cagataacct gaagaagtcc ggtgcatttg taccaggaat tcgtccggga
    12541 gagcaaacgg cgaagtatat cgataaagta atgacccgcc tgaccttggt tggtgcgctg
    12601 tatattacct ttatctgcct gatcccggag ttcatgcgtg atgcaatgaa agtaccgttc
    12661 tacttcggtg ggacctcact gcttatcgtt gttgtcgtga ttatggactt tatggctcaa
    12721 gtgcaaactc tgatgatgtc cagtcagtat gagtctgcat tgaagaaggc gaacctgaaa
    12781 ggctacggcc gataattggt cgcccgagaa gttacggaga gtaaaaatga aagttcgtgc
    12841 ttccgtcaag aaattatgcc gtaactgcaa aatcgttaag cgtgatggtg tcatccgtgt
    12901 gatttgcagt gccgagccga agcataaaca gcgccaaggc tgattttttc gcatattttt
    12961 cttgcaaagt tgggttgagc tggctagatt agccagccaa tcttttgtat gtctgtgcgt
    13021 ttccatttga gtatcctgaa aacgggcttt tcagcatgga acgtacatat taaatagtag
    13081 gagtgcatag tggcccgtat agcaggcatt aacattcctg atcataagca tgccgtaatc
    13141 gcattaactt cgatttatgg cgtcggcaag acccgttcta aagccatcct ggctgcagcg
    13201 ggtatcgctg aagatgttaa gatcagtgag ctgtctgaag gacaaatcga cacgctgcgt
    13261 gacgaagttg ccaaatttgt cgttgaaggt gatctgcgcc gtgaaatcag catgagcatc
    13321 aagcgcctga tggatcttgg ttgctatcgc ggtttgcgtc atcgtcgtgg tctcccggtt
    13381 cgcggtcagc gtaccaagac caacgcacgt acccgtaagg gtccgcgcaa accgatcaag
    13441 aaataatcgg ggtgattgaa taatggcaaa ggcaccaatt cgtgcacgta aacgtgtaag
    13501 aaaacaagtc tctgacggcg tggctcatat ccatgcttct ttcaacaaca ccatcgtgac
    13561 tatcactgat cgtcagggta acgcgttggg ttgggcaaca gccggtggtt ccggtttccg
    13621 tggttctcgc aaatccactc cgtttgcagc tcaggttgca gcagagcgtt gcgctgacgc
    13681 cgtgaaagaa tacggcatca agaatctgga agttatggtt aaaggtccgg gtccaggccg
    13741 cgaatctact attcgtgctc tgaacgccgc aggtttccgc atcactaaca ttactgatgt
    13801 gactccgatc cctcataacg gttgtcgtcc gccgaaaaaa cgtcgcgtat aacgcctcgt
    13861 tttccaggtt tgttggagaa agaaaatggc aagatatttg ggtcctaagc tcaagctgag
    13921 ccgtcgtgag ggcaccgact tattccttaa gtctggcgtt cgcgcgatcg ataccaagtg
    13981 taaaattgaa caagctcctg gccagcacgg tgcgcgtaaa ccgcgtctgt ctgactatgg
    14041 tgtgcagttg cgtgaaaagc aaaaagttcg ccgtatctat ggtgtgctgg agcgtcagtt
    14101 ccgtaactac tacaaagaag cagcacgtct gaaaggcaac accggtgaaa acctgttggc
    14161 tctgctggaa ggtcgtctgg acaacgttgt ataccgtatg ggcttcggtg ccactcgtgc
    14221 agaagcacgt cagctggtta gccataaagc aattatggta aacggtcgtg ttgttaacat
    14281 cgcttcttat caggttagtc cgaatgacgt tgtaagcatt cgtgagaaag cgaagaagca
    14341 gtctcgcgtg aaagccgctc tggagctggc tgagcagcgt gaaaagccaa cttggctgga
    14401 agttgatgct ggcaagatgg aaggtacgtt taagcgtaag ccggagcgtt ctgatctgtc
    14461 tgcggacatt aacgaacacc tgatcgtcga gctttactcc aagtaaagct tagtaccaaa
    14521 gagaggacac aatgcagggt tctgtgacag agtttctaaa accgcgcctg gttgatatcg
    14581 agcaagtgag ttcgacgcac gccaaggtga cccttgagcc tttagagcgt ggctttggcc
    14641 atactctggg taacgcactg cgccgtattc tgctctcatc gatgccgggt tgcgcggtga
    14701 ccgaggttga gattgatggt gtactacatg agtacagcac caaagaaggc gttcaggaag
    14761 atatcctgga aatcctgctc aacctgaaag ggctggcggt gagagttcag ggcaaagatg
    14821 aagttattct taccttgaat aaatctggca ttggccctgt gactgcagcc gatatcaccc
    14881 acgacggtga tgtcgaaatc gtcaagccgc agcacgtgat ctgccacctg accgatgaga
    14941 acgcgtctat tagcatgcgt atcaaagttc agcgcggtcg tggttatgtg ccggcttcta
    15001 cccgaattca ttcggaagaa gatgagcgcc caatcggccg tctgctggtc gacgcatgct
    15061 acagccctgt ggagcgtatt gcctacaatg ttgaagcagc gcgtgtagaa cagcgtaccg
    15121 acctggacaa gctggtcatc gaaatggaaa ccaacggcac aatcgatcct gaagaggcga
    15181 ttcgtcgtgc ggcaaccatt ctggctgaac aactggaagc tttcgttgac ttacgtgatg
    15241 tacgtcagcc tgaagtgaaa gaagagaaac cagagttcga tccgatcctg ctgcgccctg
    15301 ttgacgatct ggaattgact gtccgctctg ctaactgcct taaagcagaa gctatccact
    15361 atatcggtga tctggtacag cgtaccgagg ttgagctcct taaaacgcct aaccttggta
    15421 aaaaatctct tactgagatt aaagacgtgc tggcttcccg tggactgtct ctgggcatgc
    15481 gcttggaaaa ctggccaccg gcaagcatcg ctgacgagta accggatcac aggttaaggt
    15541 tttactgaga aggataaggt catgcgccat cgtaagagtg gtcgtcaact gaaccgcaac
    15601 agcagccatc gccaggctat gttccgcaat atggcaggtt cactggttcg tcatgaaatc
    15661 atcaagacga ctctgcctaa agcgaaagag ctgcgccgcg tagttgagcc gctgattact
    15721 cttgccaaga ctgatagcgt tgctaatcgt cgtctggcat tcgcccgtac tcgtgataac
    15781 gagatcgtgg caaaactgtt taacgaactg ggcccgcgtt tcgcgagccg tgccggtggt
    15841 tacactcgta ttctgaagtg tggcttccgt gcaggcgaca acgcgccgat ggcttacatc
    15901 gagctggttg atcgttcaga gaaagcagaa gctgctgcag agtaatctga agcaacgtaa
    15961 aaaaacccgc cccggcgggt ttttttatac ccgtagtatc cccacttatc tacaatagct
    16021 gtact
//
LOCUS       DAJTYG010000083        15929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTYG010000083 DAJTYG010000000
VERSION     DAJTYG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.083"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..882)
                     /locus_tag="OO568_002932"
     CDS             complement(<1..882)
                     /locus_tag="OO568_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6642214.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGA"
     gene            989..1285
                     /locus_tag="OO568_002933"
     CDS             989..1285
                     /locus_tag="OO568_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6642215.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="OO568_002934"
     CDS             complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="OO568_002934"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6642216.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="OO568_002935"
     CDS             complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="OO568_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6642217.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="OO568_002936"
     CDS             complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="OO568_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6642218.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            4297..4380
                     /locus_tag="OO568_002937"
     CDS             4297..4380
                     /locus_tag="OO568_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6642219.1"
                     /translation="MIDKAIIVLGALIVLLELIRFLLQLLN"
     gene            complement(4434..5786)
                     /gene="gorA"
                     /locus_tag="OO568_002938"
     CDS             complement(4434..5786)
                     /gene="gorA"
                     /locus_tag="OO568_002938"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6642220.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5858..6700)
                     /gene="rlmJ"
                     /locus_tag="OO568_002939"
     CDS             complement(5858..6700)
                     /gene="rlmJ"
                     /locus_tag="OO568_002939"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6642221.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6903..8945
                     /gene="prlC"
                     /locus_tag="OO568_002940"
     CDS             6903..8945
                     /gene="prlC"
                     /locus_tag="OO568_002940"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6642222.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8953..9705
                     /gene="rsmJ"
                     /locus_tag="OO568_002941"
     CDS             8953..9705
                     /gene="rsmJ"
                     /locus_tag="OO568_002941"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6642223.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9754..11223)
                     /gene="dtpB"
                     /locus_tag="OO568_002942"
                     /pseudo
     CDS             complement(9754..11223)
                     /gene="dtpB"
                     /locus_tag="OO568_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(11529..11963)
                     /gene="uspA"
                     /locus_tag="OO568_002943"
     CDS             complement(11529..11963)
                     /gene="uspA"
                     /locus_tag="OO568_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6642224.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12354..12689
                     /gene="uspB"
                     /locus_tag="OO568_002944"
     CDS             12354..12689
                     /gene="uspB"
                     /locus_tag="OO568_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6642225.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12760..14259)
                     /gene="pitA"
                     /locus_tag="OO568_002945"
     CDS             complement(12760..14259)
                     /gene="pitA"
                     /locus_tag="OO568_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6642226.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            14491..15690
                     /locus_tag="OO568_002946"
     CDS             14491..15690
                     /locus_tag="OO568_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6642227.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         4027 a         4057 c         4169 g         3676 t
ORIGIN      
        1 tgcgccggca ctgccgatga ttaatgccat taatgctccc agacccattc cttttgtcat
       61 cagaacagat gccagaggga taaccgcctc agcccggata tacagcggaa taccgacaac
      121 ggcgcttaat gggatggcga agggattatc tgctcctgca tgagcggcaa tccacgctga
      181 gggaataaag ccataaataa aagatcctat taatactcct aacaaaagat atggcagtac
      241 atctttaaac tgttgcaacg catctttggt ggctagtttt atgggattaa tacttactac
      301 agctttggta ctgcaacagg taactacggg gttctcaatg gctttagccg ctggactaca
      361 acaactcact tttatgggcg tatatgtcgt tcccggcgaa gtttttgctg gagcacaaca
      421 atttgctgat gagcttttac tggcaatgat atgacgttca aaccccagag aatccaggat
      481 aatactggca agaacggaga cgccggctgc gataatcgcg tacaacaagg tgactttcca
      541 accaaaggtc atccacatta gtccgacgat aatgggatta agtaatggtg aaacaaataa
      601 aaaagtgaga gtcggaccaa acccggcttt cgctgacaac aatccacgta acatgggtat
      661 tgtcgagcaa ctacagaacg gggtaacggc tcccaacaga gcagccagga gatagcctct
      721 cccttttctg gcccccatca tctgctggat tttatggtct ggcacctttt gtcttatcag
      781 gctgacaccg gcactaatca ctataaacag caacgaaagc tcgacggcga gaaacacaaa
      841 catctctgcg gcatcttgca gcatcgcaag ccaactactc atacgtaacc tcattggata
      901 aatctatatt tccagaataa tcgaaatgaa attcagatca agatatttct ggtattatcg
      961 aaatatcaac cagacagagg ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa
     1021 agagctaggt cacccgaccc gtttgttcat atttaaacat cttgtcaaag ccggggaaca
     1081 agggttgccg gtcggtgagt tgcaaaagca gcttgggata cctggctcca cactcagcca
     1141 ccatatatca gcactggtat cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct
     1201 gatgtgtgtt tcgcaatacg caatgctgga agcgataatt gaattcttac gcgaggagtg
     1261 ctgcgtaaat agcaaaattg cctgatgcgc tacgctctac atatttcatg ccatatattg
     1321 aatttgcacg gttttgtagg tcggataaga cgcgttagcg tcgcatccga cataaacaaa
     1381 gcgcacttta ccaactatcg gcagtggata tcgccgcttt atttcaggcg cttacccgct
     1441 tcatcaacca ctttctcgcc atcttccttg gagaatgcgc ctttttgcgc atctggcaga
     1501 atttccagca ccacttctga agggcggcac aggcgagttc ccagcggcgt caccacaatc
     1561 gggcgattaa tcagaatcgg gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat
     1621 ttatcttccg caaggcccag ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt
     1681 acggaaatcc ccatatcggc aatgagtttg accagttcat cgcgcgttgg cggagtttcc
     1741 agataatgga taatggtcgg ttctgtgccg ctgttgcgga tcatctccag cgtattacgc
     1801 gacgtgccgc aggccgggtt gtgataaatg gtaatgttgc tcatatcagt atctcattac
     1861 aaagtgaaag agagacgtag cgccagcgca gccagcgtca caaacagcac aggcagggtc
     1921 atgataatcc ctgtacggaa gtaatatccc cagctgatag tcatattctt ctgcgaaagt
     1981 acgtgcagcc agagcagcgt tgccaggcta ccaatcgggg taattttcgg ccccaaatcg
     2041 cagccaatca cgttggcata aaccatcgct tctttgataa cgccagatgc cgtgctgcca
     2101 tcaatggaca acgcgccaac cagtaccgtc ggcatattgt tcataataga agagaggaag
     2161 gcggtgagga atccggtgcc gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg
     2221 agtacgccag aaagatattc cgttaatccg gcattgcgca ggccataaac caccagatac
     2281 atgccgagcg agaagatgac aatctgccag ggggcaccgc gcaggacttt acccgtatta
     2341 atcgcatgac cgcgtttagc gacgacaaat aatatcagtg cgcccacagc tgcaatggcg
     2401 ctcaccggaa tgccgagcgg ttccaggacg aaaaatccca ccagcagaag cagtaaaaca
     2461 acccagccag ttttgaacgt agcaggatct ttgatcgctt ctgcgggaga tttcagcagc
     2521 gccatatcgt agttctgcgg aatatctttg cgaaaataga gatgtagcat caccagcgtg
     2581 gcaacaatcg cggcgatatc caccggcacc atcaccgagg cgtattcgcg aaagccgagg
     2641 ccaaagaaat cagcggaaac gatattcacc aggttggaga caataagcgg caggctggcg
     2701 gtatcggcaa tgaatccggc cgccatcacg aacgccagcg tcgtgccttt actgaatcct
     2761 aaagccagca gcatggcgat gacaatcggc gtcaaaataa gcgccgcgcc atcattggca
     2821 aacagggccg caacggcggc accgagcagg acaatccagg taaacagcaa gcgaccgcgt
     2881 ccattgcccc agcgtgaaac gtgcagcgct gcccattcaa aaaagccgga ctcatccagc
     2941 agcaggctga tgataatgac ggcaataaac gccgccgtcg cgttccagac gatattccac
     3001 accaccggaa tatcacccgg atgaaccacg cccgtaacca acgccagtac cgcgccgagc
     3061 gttgcactcc agccgatgcc taaacctttc ggctgccaga taaccaatac gatggtcagg
     3121 acaaagatag cgcctgccag taacataatg cctcccggat aaaacacatg tgcaaatcca
     3181 tatgtgttta gctaaatttt taactgcaaa tgttcttact gtctccggaa cagttttgtc
     3241 tggccaggtt gcggacaatt gcctgaatct tttcctgttc acatcgccag gcttcatcaa
     3301 taattttcgc cgcccatgct ggaatatgcg gcgataagcg gtaatgaacc cacttacctt
     3361 gcttgcggtc cagcaatagc ccgctttcac gcagcaatgc cagatggcgg gatatcttgg
     3421 gctgcgactg atcgagggca gtgcagagat cgcagacgca taactctccc agttcgctaa
     3481 gcagtaaaac gatgcccaga cgggtttcat cagcaagaat tttgaacaat tggatgggta
     3541 acagaaatga catattgcgc tcctgattgt tgcaggtagt gtctctcttc gaagcggata
     3601 agtcaaaaac atatatgact taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa
     3661 ttttgagttg gctattcatt tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt
     3721 gcgtcggttt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     3781 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     3841 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     3901 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     3961 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     4021 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     4081 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     4141 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     4201 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     4261 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     4321 cttggggcgt taattgtgct gctggaactg atccgctttc tgcttcagct tctgaactga
     4381 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     4441 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     4501 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     4561 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     4621 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     4681 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     4741 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     4801 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     4861 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     4921 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     4981 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     5041 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     5101 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     5161 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     5221 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     5281 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     5341 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     5401 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     5461 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     5521 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     5581 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     5641 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     5701 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     5761 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     5821 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     5881 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     5941 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     6001 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     6061 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     6121 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     6181 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     6241 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     6301 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     6361 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     6421 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     6481 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     6541 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     6601 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     6661 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     6721 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     6781 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     6841 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     6901 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     6961 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     7021 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     7081 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     7141 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     7201 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     7261 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     7321 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     7381 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     7441 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     7501 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     7561 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     7621 gcacccgcgc ctccgatcaa ggcccgaacg ccggtaagtg ggataacagc aaggtgatgg
     7681 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     7741 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     7801 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     7861 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     7921 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     7981 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     8041 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     8101 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     8161 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     8221 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     8281 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     8341 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     8401 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     8461 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     8521 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     8581 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     8641 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     8701 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     8761 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     8821 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     8881 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     8941 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     9001 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     9061 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     9121 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     9181 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     9241 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     9301 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     9361 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     9421 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     9481 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     9541 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     9601 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     9661 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     9721 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     9781 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     9841 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     9901 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     9961 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
    10021 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
    10081 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagtcgt
    10141 caaaaagccc agtgagcaca taaacatgcc gagagtaaat ttcatcggca tcgagaggtc
    10201 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
    10261 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
    10321 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
    10381 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
    10441 atccagcttg aatgcctgac gaaagaagat gatggtgacg acgatggaga gaacaatcag
    10501 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
    10561 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
    10621 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
    10681 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
    10741 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
    10801 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
    10861 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
    10921 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
    10981 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
    11041 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
    11101 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
    11161 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
    11221 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
    11281 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
    11341 attacaacag aaaataacca gctgatcaca ctaatgatgg tgttaaattt tgtattggca
    11401 ggagattttg attgcgtacg cagatcgtag gccagataag gggtttacgc tgcatcaggt
    11461 aacaaggcgc gccaaaaagc aaaacgcccg catatgcggg cgttcaggaa cacgtcgtgg
    11521 agggatgatt attcttcttc gtcgcgcagc ggaacaatca gcatatcaac gtgaacggtg
    11581 ttgatcagct gacgtgcgga agacatcagt ttgctccaga agtcctggtg gtgaccacaa
    11641 accaccagat ccatatcgta tttcttgatt gcatcgacca gaacctggcc caggtcgccg
    11701 ctgccgctca gggtttcagt gattggatag cctgcattag tggaaagctc ggtcagcgca
    11761 tgatgtgtct cttcagagat gcgtttctgc atatcaccca gattcacatc aataagcccg
    11821 gtgtataggt cagagtagtt tacatctacg tggatcagag aaactttcgc attgtagggg
    11881 cgagccatag agactgcttt ctctaccaga actttgcttt ccggggagag atcgactgcg
    11941 atgagaatgt gtttataagc catagtgtta ctccttccat aaagttgtcg atgactggcc
    12001 agctagcgtt tcttgtgccg ttacgtcacc cgcgtcctgc gaactacgtg cgcggggcta
    12061 acccagccgt aaagattcag tgttaagacc atccttacct tatagcgacc cggatgatcc
    12121 gtcaatccgc cttgcttacc cgttagataa acaaattttc gacatgaata tattgatagt
    12181 ggttaacctt ctggaaaaaa aacaacctga tctcctacac tatctataga gccgctcgga
    12241 tgttggtcac cacaaaagcg gtttctccag gacctggtgt taactgattg gcagcgtatc
    12301 gggagcggta gccccggggt ggaactccgc gggcaggtcg ccggggagga ggtatgataa
    12361 gcaccgtcgc attattttgg gctttatgtg tcgtttgcat tgttaacatg gcgcgctatt
    12421 tctcatcact acgcgcgttg ttagtggtac tgcgtaactg cgatccattg ctctatcaat
    12481 atgttgatgg agggggcttt tttacctcac atggccaacc caacaaacag gtgcgtctcg
    12541 tttggtatat ctatgcccaa cgttatcgcg atcatcacga tgatgagttt attcgccgct
    12601 gtgagcgggt gcgtcggcag tttattctga ctagcgcatt gtgtggtctg gtggtggtca
    12661 gcctgattgc attgatgatt tggcattaag cacttcagca ataataacgc ataaaaaaag
    12721 cgggccagta aactgacccg ctcgttttgg tgcgtacgat tacaggaact gcaaggagaa
    12781 ccagtacagc ccgccggaaa gtagtaccgc agccggaagg gtaaacaccc aagccatcag
    12841 aatactggtt acggttttac gctgtaagcc gccgccatct accaccatcg tccccgcgac
    12901 agaagaggag agtacgtgag tggtggaaac cggcatcccg gtataactcg ccaggccgat
    12961 tgacactgcc gccgtcatct gggcagacat cccctgagcg taggtcatgc ctttcttacc
    13021 gattttctca ccgatagtcg ttgccacacg acgccagcca atcatcgtac cgatacctaa
    13081 cgccagcgcg accgccatga tgatccacac cggcgcatac tcgatggtgc taagcatgtc
    13141 ggacttcagt ttcttcaaca ggcgctgatc gtcagcactc acaccaggca tcttcaccac
    13201 tttgtcgata gtgtcagaaa cgcacagcat aatgcggcgc atctggctac gttgatcaag
    13261 cgacagcttg tcgtagcttt ccacatcggt ggtcaacata cctttcaggc ggttgagcgc
    13321 gttaatggta ttcgacggat ggcagtggaa ctccgcaggt tgcgttgcgc cagcttccgg
    13381 agccggtact aactgatcag caccggtagc ctgtttgagc agcgcaggat gctgctcaaa
    13441 gtaagcttcg acgttgttga tggcatcacg ggtacgggtg atttcgtagc cagtggcatt
    13501 catgttcacc acgaagcctg ctggcgcgac gccaatcaat accaacataa ccagaccaat
    13561 gcctttctga ccatcgttcg cgccgtgcga aaacgccacg ccgatagcgg aaaggatcag
    13621 cgcgatacgc gtccagaacg gcggcttttt cttgccgtct ttcttttcac gctccgctgg
    13681 ggtcaggtgg atacgggcgc gtttcttggt gccgctccag tagcgacgca gcaagaaaat
    13741 cagaccgcca gcaaacacca ggccgacaat aggggaaacg atcagagaac cgaaaatact
    13801 taatactttc gggatattga gtgcatccac cactgacgtc ccggtcatca acgcattggt
    13861 taaaccaatc ccgatgatcg cgccaatcag cgtatgagag ctggatgcag gtaaaccaaa
    13921 gtaccaggta cccaggttcc agataatcgc cgccagcaac atagagaaca ccatggcaag
    13981 gccatgagac gatcccatat taagcagcag atccgtcggc agcatatgca caatggcata
    14041 ggcaacactc agaccaccca gcaaaacacc caaaaagttg aacaccgccg ccataaccac
    14101 ggcgagctga gaacgcatcg cgcgggtata gataacggtt gccacggcgt tggctgtgtc
    14161 atggaaacca ttgatggctt cgtagaacag cacaaaagcc agtgcaagca ataataacag
    14221 cccggtatgc aaatccaggc cagcaaacaa atgtagcata ggacgttacg ccattttgag
    14281 gacatgaacg cggcgcatta tcagtgactt tcgcggcgcg ggcaaagtga aatatagact
    14341 ttttttgatt tgcctcctgt atggatttca ctcaaaaaat aattatctta tataattcag
    14401 gcaaatactt ccttttagta atattgatgc tggtgcgacc actgaggaat ctttacaatt
    14461 cacgcccgtt ttttctaaga ggagcgcaac gtggaaaggt ttgatgccat tattataggc
    14521 gctggtgcgg cgggtatgtt ctgttctgcg ctggcaggtc aggcaggacg ccgggttctg
    14581 ctgatcgata atggtaaaat actagggcgc aaaatcctta tgtctggcgg tgggcgctgc
    14641 aactttacca acctttatgt cgaaccaggc gcttatctga gccagaatcc gcatttttgt
    14701 aagtctgcac tcgcacgttt tacccagtgg gatttcattg atctggtcaa taaacacggt
    14761 atcgcctggc acgagaaaac gttagggcaa ctcttctgcg atgactccgc gcagcagatt
    14821 gtcgacatgc tggtggatga gtgcgagaag ggcaatgtaa ccttcagatt gcgtagcgaa
    14881 gtgctgagtg tggcgaagga tgaaacaggc ttcacgcttg atctgaacgg catgactgtc
    14941 ggttgcgaaa agctggtcat cgcgaccggt gggctgtcaa tgccggggct gggcgcatcg
    15001 ccgtttggtt ataagattgc cgaacaattt ggcctcaacg tgctgccgac ccgcgcgggt
    15061 ctggtgccat tcactctgca taaaccgttg ctcgaagagt tacaggtgct ggcgggcgtg
    15121 gcggtgcctt ccgtgattac cgctgaaaac ggcaccgttt tccgtgagaa cttactcttc
    15181 acccaccgcg gcttatctgg accggcggtg ttgcagattt caagctactg gcaaccgggt
    15241 gaatttgtca gtatcaatct gctaccggat gtggacctcg aaaccttcct gaatgagcag
    15301 cgtaacgcac atccgaatca aagcctgaaa aacacactgg cggttcatct accgaagcgg
    15361 ttggttgaac gcttacagca actcgggcaa atcccggatg tttcgctaaa acagctcaac
    15421 gtgtgtgacc aacaggcact gattagcaca ttgaccgact ggcgcgtaca acccaacggc
    15481 actgaaggct atcgcactgc cgaagtgacg ctcggcggcg tggacaccaa cgaactttct
    15541 tcacggacga tggaagcgcg caaagtgcct gggctgtact tcatcggcga agtgatggac
    15601 gttaccggct ggctgggggg ctataacttc cagtgggcgt ggtcgagtgc atgggcttgt
    15661 gcgcaggatt tggcgtcaat tgtgaaataa ccatgacgtt ttcccgtcag ccgtttgtat
    15721 atgtcgttat taagtcattt tggtacggtt ctgcatccga cattctggtg ttaactcgct
    15781 gttttgttag cgaattaacg ctattgccgt tgtggttatt ttatagtgta tttattctca
    15841 gagaatatat attactgaaa taaaaaacac aaagaataac caaaggaaat tatttgaaac
    15901 gtaaaaggta tatattttta atctgtaaa
//
LOCUS       DAJTYG010000084        15883 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTYG010000084 DAJTYG010000000
VERSION     DAJTYG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15883
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.084"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..236
                     /locus_tag="OO568_002947"
     CDS             <1..236
                     /locus_tag="OO568_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR6642228.1"
                     /translation="LGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPA
                     LSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            246..884
                     /locus_tag="OO568_002948"
     CDS             246..884
                     /locus_tag="OO568_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6642229.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            887..2050
                     /locus_tag="OO568_002949"
     CDS             887..2050
                     /locus_tag="OO568_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6642230.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2134..3758
                     /locus_tag="OO568_002950"
                     /pseudo
     CDS             2134..3758
                     /locus_tag="OO568_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
     gene            complement(3875..4150)
                     /gene="yjfN"
                     /locus_tag="OO568_002951"
     CDS             complement(3875..4150)
                     /gene="yjfN"
                     /locus_tag="OO568_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6642231.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4299..4628)
                     /gene="bsmA"
                     /locus_tag="OO568_002952"
     CDS             complement(4299..4628)
                     /gene="bsmA"
                     /locus_tag="OO568_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6642232.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4810..5559
                     /gene="yjfP"
                     /locus_tag="OO568_002953"
     CDS             4810..5559
                     /gene="yjfP"
                     /locus_tag="OO568_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6642233.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5556..6311)
                     /gene="ulaR"
                     /locus_tag="OO568_002954"
     CDS             complement(5556..6311)
                     /gene="ulaR"
                     /locus_tag="OO568_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6642234.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6419..7483)
                     /gene="ulaG"
                     /locus_tag="OO568_002955"
     CDS             complement(6419..7483)
                     /gene="ulaG"
                     /locus_tag="OO568_002955"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6642235.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCANDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7838..9235
                     /gene="ulaA"
                     /locus_tag="OO568_002956"
     CDS             7838..9235
                     /gene="ulaA"
                     /locus_tag="OO568_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR6642236.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9251..9556
                     /gene="ulaB"
                     /locus_tag="OO568_002957"
     CDS             9251..9556
                     /gene="ulaB"
                     /locus_tag="OO568_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6642237.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9566..10030
                     /gene="ulaC"
                     /locus_tag="OO568_002958"
     CDS             9566..10030
                     /gene="ulaC"
                     /locus_tag="OO568_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6642238.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAVVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10044..10694
                     /gene="ulaD"
                     /locus_tag="OO568_002959"
     CDS             10044..10694
                     /gene="ulaD"
                     /locus_tag="OO568_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6642239.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10704..11539
                     /gene="ulaE"
                     /locus_tag="OO568_002960"
                     /pseudo
     CDS             10704..11539
                     /gene="ulaE"
                     /locus_tag="OO568_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
     gene            11539..12225
                     /gene="ulaF"
                     /locus_tag="OO568_002961"
     CDS             11539..12225
                     /gene="ulaF"
                     /locus_tag="OO568_002961"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6642240.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTL
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12354..12629)
                     /locus_tag="OO568_002962"
     CDS             complement(12354..12629)
                     /locus_tag="OO568_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6642241.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12956..13351
                     /gene="rpsF"
                     /locus_tag="OO568_002963"
     CDS             12956..13351
                     /gene="rpsF"
                     /locus_tag="OO568_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6642242.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNIEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13358..13672
                     /gene="priB"
                     /locus_tag="OO568_002964"
     CDS             13358..13672
                     /gene="priB"
                     /locus_tag="OO568_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6642243.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13677..13904
                     /gene="rpsR"
                     /locus_tag="OO568_002965"
     CDS             13677..13904
                     /gene="rpsR"
                     /locus_tag="OO568_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6642244.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13946..14395
                     /gene="rplI"
                     /locus_tag="OO568_002966"
     CDS             13946..14395
                     /gene="rplI"
                     /locus_tag="OO568_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6642245.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14466..15029)
                     /locus_tag="OO568_002967"
                     /pseudo
     CDS             complement(14466..15029)
                     /locus_tag="OO568_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            15093..15883
                     /locus_tag="OO568_002968"
                     /pseudo
     CDS             15093..15883
                     /locus_tag="OO568_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3730 a         3877 c         4277 g         3999 t
ORIGIN      
        1 tgttaggtta cgttatccca ttatccagtg cggcaatcaa tgcggtgagt attccagact
       61 atttcgcctg gggcgggatt gcactggtga ttcagttact cgtttttgct ggtgtcagac
      121 tttatatgcc tgcattaagc gaaaaaatta ttaatcacaa taccgcagca ggaatgttta
      181 tgggaaccgc cgcgctggct ggcggtattt ttaacgcagc ttgtatgaca tggtaatgga
      241 cgatcatggc cagaaaacgc aaatcaagaa ataacagtaa aattggtcac ggagcgatca
      301 gtcgcattgg tagaccgaat aatccttttg aaccgcgtcg caatcgctac gcacaaaaat
      361 acttaacgtt ggcgctaatg ggcggtgccg cttttttcgt attgaaaggt tgtagcgata
      421 gtagcgatgt cgataacgac ggcgacggaa cgttttacgc gacagtgcag gattgtattg
      481 atgacggtaa taatgccgac atttgcgcgc gtggctggaa caacgccaaa gcggcatttt
      541 atgccgatgt tccaaagaat atgactcagc agaactgtca gtctaagtac gaaaattgct
      601 actacgacaa tgttgaacag agttggatcc cggtcgtttc tggatttttg ttaagccggg
      661 ttattcgtaa agatcgcgat gagccgttcg tctataacag cggcggatcc tcatttgctt
      721 cccgccccgt ctggcgcaac acttccggtg attactcctg gcgcttcggt tctggcaaaa
      781 aagagtctta ctcttcaggc ggctttacca ccagaaaagc gtcgaccgtt tcgcgcggcg
      841 gctatggtcg ttcttccagc gcccgtgggc attggggagg ctaatcatgc tgagacacaa
      901 cgttcctgtg cggcgggatc tggaccagat cgccgctaac aacggtttcg actttcatat
      961 catcgacaat gaaatctatt gggacgagag tcgggcttac cgttttactc tgcgccagat
     1021 tgaagagcag atcgaaaaac cgactgcgga actgcatcag atgtgccttg aggtggtgga
     1081 tcgcgcggtt aaagacgaag agatcctgac gcaactggcg atcccgccgt tgtactggga
     1141 tgtgatcgcc gaaagctggc gcgcacgcga tccttcgctg tatggccgga tggattttgc
     1201 ctggtgtggc aatgcgccgg tgaagctgct ggagtacaac gccgatacgc cgacctcgtt
     1261 gtatgagtcg gcttatttcc agtggttgtg gctggaggat gcccggcgca gcggcattat
     1321 tccgcgtgat gccgatcagt acaacgccat tcaggaacgc ctgatttcgc gctttagtga
     1381 gctttacagt cgggaaccgt tttatttttg ctgctgtcag gacaccgatg aagacaggag
     1441 taccgtgctg tacttgcagg actgcgccca gcaggcaggg caggagtcgc ggtttatcta
     1501 cattgaagat ctcggtttgg gcgtcggcgg cgtactgacc gatcttgatg ataatgtcat
     1561 ccagcgtgca tttaagctgt atccgctgga gtggatgatg cgtgacgata acggtccgct
     1621 gctgcgcaag cgtcgcgagc aatgggtgga gccgttatgg aaaagtatct tgagtaataa
     1681 agggctaatg ccgctgcttt ggcgcttctt ccctggtcat cctaaccttc ttgcatcctg
     1741 gtttgagggt gaaaaatcgc agattgccgc tggcgaaagc tatgtgcgta aaccgatcta
     1801 ctcgcgcgaa ggcggaaacg tcaccatttt cgacggtcag aataacgtcg ttgaccacgc
     1861 tgatggtgat tacgccgatg aaccgatgat ctaccaggcg tttcaacctc tgccgcgatt
     1921 tggcgatagc tacacactca tcggtagctg gattgtcgat gatgaagcgt gcggaatggg
     1981 gatccgtgaa gataacacac tgatcaccaa agacacctca cgtttcgttc cgcattacat
     2041 tgctggataa gaatgtttta gcaatctctt tctgtcatga atccatggca gtgaccatac
     2101 taatggtgac tgccattgat ggagggagac acagtgcact ggcaaactca caccattttt
     2161 aatcaaccta taccattaaa taacagcaac ttatacctgt ctgatggcgc gctttgcgaa
     2221 gcggtaacgc gtgaaggtgc tggctgggat agcgattttc tagccagtat tggtcagcag
     2281 ttaggaacgg ctgaatccct tgaactgggg cggctggcga atgtaaaccc gcctgaactg
     2341 ttgcgctacg atgcccaggg gcggcgtctg gacgatgttc gtttccatcc cgcatggcac
     2401 ctgctgatgc aggcgttatg taccaatcgg gtgcacaatc ttgcctggga agaagacgct
     2461 cgctccggcg catttgtggc gcgcgcggcg cgttttatgt tacatggcag gttgaggctg
     2521 ggtcgttatg tccaataacg atgacctttg ccgccacgcc attgttgtta cagatgttac
     2581 ccacgccgtt tcaggactgg accacgccgc tgctgagcga tcgctacgat tctcacttat
     2641 tgccaggtgg gcaaaaacgc ggtttgttga ttggcatggg aatgacggaa aagcagggcg
     2701 gttccgatgt catgagcaac accatccgtg cagagcgttt ggaagatggc tcttatcggc
     2761 tggtggggca taaatggttt ttctcggtgc cgcaaagcga tgcgcatctg gtgctggcgc
     2821 agaccgcggg cggtctgtcc tgcttttttg tgccgcgctt tttgcctgac gggcaacgca
     2881 acgcgattcg cctcgagcgg ctgaaagata agctgggtaa tcgctctaac gccagttgcg
     2941 aagtggagtt tcaggatgcc attggctggt tattggggca ggaaggggaa ggaattcgtc
     3001 tgatcctgaa aatggggggg atgacgcgtt ttgattgcgc cctgggtagc catgccatga
     3061 tgcgccgtgc attttcgctg gcgatttatc atgcacatca acgccatgtt tttggtaatc
     3121 cattgatcca acagcccctt atgcgtcatg tcttaagtcg catggcactt cagcttgaag
     3181 ggcaaacggc gttgctgttt cgtcttgcgc gagcgtggga ccggcgtgcc gatgccaaag
     3241 aagccctgtg ggcgcgttta tttacgcctg cggcaaaatt tgtgatctgc aaacgaggta
     3301 tgccgtttgt ggccgaagcg atggaggtgc tgggcggcat tggttattgc gaggagagcg
     3361 agctgccgcg gctttaccgg gagatgccgg taaacagtat ttgggaaggt tccggcaata
     3421 ttatgtgcct ggatgtgttg cgcgttctca ataagcaagc gggcgtatac gacttattgt
     3481 cggaagcatt tgtggaagtg aaagggcagg atcgctattt tgatcgcgcg gttcgtcgtt
     3541 tacagcagca gctgcgtaag ccagctgaag aactggggcg agagattact catcagctat
     3601 tcctgctggg ctgcggtgcg caaatgttga aatatgcttc tccgccaatg gcgcaggcgt
     3661 ggtgtcaggt gatgttagat acgcgcggcg gcgtacggtt gtcagagcag atccagaatg
     3721 atttattgct gcgggcgacg gggggagtgt gtgtgtaagc gtatacgact gatgcgacgc
     3781 tggtttcgat taactaaatg aaatatgtga aaattgtagg ccggacaagg cgctcgcgcc
     3841 gcatccggca ttgttcattt gctgtagatg acactcatgc atacagtatc gcctgtacgc
     3901 gccagttatc aggctgctgg tcttcgtaca tggtaataat gcggtaccat gacgcacctt
     3961 gttcatcggc ctttaacgcc acgatacgtt ccacgtcctg cggatcccct gaaatattgc
     4021 taactgagat ctgacctatt tcattcaggc ccgtcacgca gtcagcactg gcgaattctg
     4081 cagactgtgc cgtggcgctc gcaggataag tcactaattg cagcgagggt gaggcaagta
     4141 attgtttcat cgtcagctcc atttttcgtt tccccacatg ttgcagggca ttccatcgcg
     4201 gtaataaacg cccaagcttc cagccggtaa tttattgtgc acaggtaaac gcatagggac
     4261 gcaaagcaat gtaaagctga ctatgtaagt tgtctgattt atttacggta caaaatggct
     4321 tgtgaatacc actgtcctgt cacgatggtt tcgtccacca tgacgacaac gtaataatca
     4381 gcttttgcag cgacagcttt tgctctgatt tctgctaatg cgtcatccgg agaaccccga
     4441 accatcgtgc ttacgctacc tattcgctgt aacccttgcg tctggtcgcg gcgaatctct
     4501 tgcggatggt ccgttactgg cggtgctggc tgtggcgtac cttgcagtgc gctacaggca
     4561 cttaacatca acaccaataa taaactggca aaccggtaaa taacgctatt acgtttcctg
     4621 ctaaccatag tgtagtgcct tattaattta actttggggg aatgttcccg aattgttacc
     4681 tggtacgcga ttttcgaatg aaaacgtgac gaaagcgtaa cccacatctc aacattatga
     4741 actaataccg ccagcagaaa cttgtgctaa ggatttcttc atgtcatacc agacaaaaag
     4801 gagagacaga tgattgaaat agaatcacgc gagctggcag atattcccgt tcttcatgct
     4861 tatcctgtcg ggcaaaaaga taccccgtta ccgtgcgtaa ttttttatca cggctttact
     4921 tcatccagcc tggtgtatag ctattttgcc gttgcgctgg cgcaggctgg tttgcgggtg
     4981 atcatgccgg atgcgcccga tcacggtagc cgttttagtg gcgacgaagc gcggcggtta
     5041 aatcaattct ggcaaatctt gctacaaagt atgcaggaat tcactacttt acgtgcggca
     5101 atagccgaag aaaactggct gcttgatgac cgtctggcag tcggtggcgc gtcgatgggc
     5161 gcgatgacgg cactgggaat taccgctcgc caccccacgg tgaaatgtac cgccagcatg
     5221 atgggatcgg gctattttac atcgctcgcc cgttcactgt ttccaccgct gatacctgaa
     5281 acggcagcac agcagaatga attcaataaa attgtcgcgc cgctggcaga gtgggaagcg
     5341 acaaaccacc tggaacaact tggggacaga cctctactgc tgtggcatgg cctcgacgat
     5401 gacgttgtgc ctgccgacga atcactacgt ttgcagcagg ccttaagcga gacgggacgg
     5461 gataaactgc taacctgttc atggcagcca ggcgtgcgtc accgcattac gcctgaggcg
     5521 ttagatgctg ccgtgacatt tttccgccag catctttaaa cacgcagaat gctgacccct
     5581 tgcgcttcca gttgttgcag gatttccggg ttagcatttt tgccggtgat cagcatatcg
     5641 atttgatcgg cacggctaaa aagcattccc gcgcgttcgc caatcttact gctatcaacc
     5701 agtaccacca gtttccctac cacgctcagc atcttctgct ctgccattgc tgtcagcata
     5761 tcggttttat acaacccttc tgcggtcagc ccttttccgc tggtaaacat ccagtgcccg
     5821 gcatagagac tgttttcgct gccctgcggg cttaaagtga tggactgact tttgttgtac
     5881 tgtccaccca taatgatcac gctgtcatgt tcttgatcga tcaggtaatt tgccagcggt
     5941 agataattag tgatgatttg cactggcttg ccacacattt cccgcccaag cagaaacgcg
     6001 gtggagccgc agttgatgac tacgctttcg cccggattaa ccagctgcga ggccgcttta
     6061 gcaatacgta ctttttcatc gtgattctgc gcctgatgca gattcatcgg cgtccagcgc
     6121 gggcgctgtt gggtaatagc ttctgcgcca ttgcgcacct ttttcagttt gccgctttcg
     6181 ccaagtttat tgatatcgcg tcgcgcagtg gcaggcgaaa ttcccagacg ctcaacgact
     6241 ttctcaacgg tcacaaagcc caattgtgcg agcatttcca ggaggatttg atgtctttgt
     6301 gcttcagtca tgagctattc cgataaaatt tgattttttt agatgatatt tgaaatagcc
     6361 aggaaatact acgccggata gcgcgaactt ctccacgcta tccggtataa acgacagatt
     6421 acaggaatga cttgaacggc agatccggtt caatagtgaa gcaatcatcg aaaccgcgcg
     6481 gatagtggta ctcgaagttg tctttatcca gcggccaggt aaatttgccg ccaacctgcc
     6541 agataaacgg cttgaagcca tacttcagac gatctttttt catctcccac agcacgcgga
     6601 tctcttgcgg atcggcctgg aagtttgacc agatgtcgtg gtggaacggg ataaccactt
     6661 tcgcattcag ggcttcaccc atacgcagca tatcggcgct ggtcattttg tcggtaatac
     6721 cgcgcgggtt ctcaccatac gatcctaacg ccacatcgat ctgatgttcg ttaccgtgct
     6781 tcgcataata gttagagtag tgggaatcgc cactgtgata tagggaaccg ccaggggttt
     6841 tgaacaggta gtttactgcg cgatcgtcca tgccatctgg cagtacgcca gccgcttttt
     6901 gatcggcagg cagggtgatc agtgcagtac ggtcgaaagc gtcaagcgca tgaatttcaa
     6961 tgtctttcac ttttacaaca tcgcccggtt tgaccacgat gcaacgctct ttcggtacgc
     7021 cccagccaat ccacaaatca acgcaggttt tcggtccaat gaatggtacg tcattagcac
     7081 agttctgcat cacggcagca gcgacgttaa cgtcgatatg atcgttgtga tcgtgagtcg
     7141 ccagtaccgc gtcgatctgg cgaatcgcaa acggatcaag aacaaacggg gtggtgcgta
     7201 ggtttggctg cagttttttc acgccagcca tgcgctgcat ctggtgaccc tgtttcatta
     7261 acgggttacc gtgactttgt ttgccagtgc cgcaccagaa atcaacgcaa acgttggtgc
     7321 caccttctga tttcaaccag atcccggtgc agcctagcca ccacatcgca aatgtgccag
     7381 gggcgacctg ctcttgttca atttcttcat tcagccagct accccactcc gggaaagtgc
     7441 tcaggatcca ggattctcgg gtgatacttt tcactttact catcgccatt taccttcatg
     7501 atagttcaat tcgaatcaat atgtgattgg ttttgattaa tcctgacact attttttcag
     7561 gaaggcaatg accatttttt gactttttgc cagggaagtt gttgttgatt ttgagtgtag
     7621 aaagatttaa tggaatgtat agataaatta actaaatgaa tttaaataaa taattttttc
     7681 gttgtgtgaa tcccactcta tccgtgtgga attatttgcg ggtcgcgcca catttaatca
     7741 taaataatct tgttgtgatt acttttgaaa attagagtgg gtgcacaaca ttccgggtgt
     7801 gcggaatacc cggttacctc ttcttcagga gatcgttatg gagatcctct acaacatctt
     7861 taccgtgttt tttaaccagg tcatgaccaa tgccccgttg ttgctgggta ttgtgacctg
     7921 tctgggctac atcctactgc gcaaaagtgt cagcgttatt attaaaggca cgattaaaac
     7981 cataattggt ttcatgttgt tgcaggcagg gtccggcatc ctcaccagca ccttcaaacc
     8041 ggtggtggcg aaaatgtccg aagtctacgg cattaacggc gcaatttccg atacttacgc
     8101 gtcgatgatg gcaaccatcg accgcatggg cgatgcctat agctgggtag gttacgcggt
     8161 gttgttagcg ctggcgctga acatctgtta cgtgctgctg cgtcgcatta ccggtattcg
     8221 taccatcatg ctgactggtc atatcatgtt ccagcaggcg gggctgattg ccgttacgtt
     8281 gtttattttc ggctactcca tgtggacaac cattatctgc acggcgattc tggtttcgct
     8341 ctactggggc attacctcca acatgatgta caagccgact caggaagtga cggacggctg
     8401 cggtttctcc atcggtcacc agcaacagtt tgcatcatta attgcctata aagttgcgcc
     8461 gttcctcggc aaaaaagagg aaagcgttga agacctcaaa ctgccaggct ggctgaatat
     8521 tttccacgac aacatcgtct ccacggcgat tgtcatgacc atcttctttg gtgccattct
     8581 gctctccttc ggtatcgaca ccgtgcaggc gatggcaggc aaagtgcact ggacggtcta
     8641 tatcctgcaa accggttttt ccttcgcggt ggcgatcttc atcatcactc agggtgtgcg
     8701 catgtttgtg gcggaactct ctgaagcgtt taacggtatc tcccagcgcc tgatccctgg
     8761 tgcggttctg gcgattgact gtgcggctat ttatagcttc gctccgaacg ccgtggtctg
     8821 gggctttatg tggggcacca tcggtcagct gattgcggtt ggcatcctgg tcgcctgcgg
     8881 ttcctcgatc ctgattatcc caggctttat cccgatgttc ttctccaacg ccaccatcgg
     8941 cgtgttcgct aaccacttcg gcggctggcg tgcggcgctg aagatttgtc tggtgatggg
     9001 gatgatcgaa atctttggtt gcgtctgggt ggtgaaactt accggtatga gtgcctggat
     9061 ggggatggcg gactggtcga ttctggcacc gccgatgatg cagggcttct tctccatcgg
     9121 tatcgccttt atggccgtca tcattgtaat tgcactggct tatatgttct tcgctggccg
     9181 cgcactgcgc gcagaagaag atgcagaaaa acaattggca gaacagtctg cttaataagg
     9241 agttttgatt atgaccgtac gtattctggc tgtgtgtggc aacggacaag gcagttccat
     9301 gatcatgaag atgaaagtgg accagttttt aacccaatca aacattgacc acacggtaaa
     9361 tagctgcgcg gttggcgaat acaaaagcga gttgagcggc gcggatatca tcatcgcttc
     9421 tacgcacatt gcgggcgaaa tcaccgtgac cggcaacaaa tacgtggttg gcgtgcgcaa
     9481 catgctctct cctgccgact ttggcccgaa actgctggaa gtgatcaaag cgcatttccc
     9541 gcaggatgtg aagtaaggac gcgccatgaa attacatgat tcgctggcgg agaataaatc
     9601 catccgcctg caggctgaag ctgagacatg gcaggacgca gtaaaaatcg gcgttgacct
     9661 gctggtggcg gcggatgttg tcgagccgcg ttactaccag gcgattctgg atgcggttga
     9721 acagcacggt ccgtactttg tgctcgcacc aggcctggca atgccgcacg gtcgcccgga
     9781 agagggcgtt aagaaaaccg gttttgcgct ggtgacgctg aaaaagccgc tggagttcaa
     9841 ccacgaagat aacgacccgg tggatatcct catcaccatg gcggtggtcg atgccaacac
     9901 gcaccaggaa gtgggcatca tgcagatcgt caacctgttt gaagatgaag aaaatttcga
     9961 ccgtttacgt gcctgccgta ccgagcagga agtactggat ctcattgacc gcaccaacgc
    10021 ggcagcttaa gaaggaattg aagatgtcat taccgatgtt gcaagtcgcg ctggacaacc
    10081 agactatgga tagcgcctac gaaaccactc gcctgattgc cgaagaagtc gacattatcg
    10141 aagtgggtac cattctgtgc gtgggcgaag gcgtgcgtgc ggttcgcgat ctgaaagcgc
    10201 tctacccgca caaaatcgtg ctggcagacg ccaaaattgc cgatgcgggc aaaattcttt
    10261 ctcgtatgtg cttcgaagcc aacgctgact gggtgaccgt gatttgttgt gcggatatca
    10321 acaccgccaa aggtgcgctg gacgtagcaa aagagtttaa cggcgacgtg cagatcgaac
    10381 tgaccggtta ctggacctgg gaacaggcgc aacagtggcg cgacgcaggt attcagcagg
    10441 tggtttatca ccgcagccgt gacgcgcagg ccgctggcgt ggcgtggggt gaagccgata
    10501 tcactgcgat caaacgtctt tccgatatgg gcttcaaagt caccgtcacc ggtggcctgg
    10561 cgctggaaga tctgccgctg ttcaaaggca ttccaattca tgtgtttatc gccggtcgca
    10621 gcatccgtga tgccgcttct ccggttgaag ccgcgcgtca gttcaaacgt tccatcgccg
    10681 aactgtgggg ctaaggagcg gatatgttgt ccaaacaaat cccgcttggc atctatgaaa
    10741 aagcgctccc cgccggggag tgctggctgg aacgcctgcg actggcaaaa acgttgggct
    10801 tcgattttgt cgagatgtcg gtagatgaaa ctgacgagcg cctgtcgcgc ctcgactgga
    10861 gccgcgagca gcgtctggcg ctggtgaatg cgattgttga aaccggcgtg cgtgtgccgt
    10921 ccatgtgcct ttctgcccat cgtcgtttcc cgttgggcag cgaagatgac gcggtgcggg
    10981 cgcaggggct ggagattatg cgtaaagcca tccagttcgc ccaggacgtt ggcattcgcg
    11041 tgatccagct gcgggctatg acgtttacta tcaggaagcc aataacgaaa cgcgtcgtcg
    11101 tttccgtgac ggcctgaaag agagcgttga gatggcgagc cgcgcacagg tgacgctggc
    11161 gatggagatc atggattatc cgttgatgaa ctccatcagc aaggcgctgg gttacgcgca
    11221 ctatctcaac aacccgtggt tccagctcta cccggatatc ggcaacctgt cggcgtggga
    11281 caacgatgtg cagatggagt tgcaggccgg aatcgggcat atcgtcgcgg tgcatgtgaa
    11341 agacaccaaa ccgggcgtct tcaaaaacgt gccatttggc gaaggtgtgg tggatttcga
    11401 acgttgcttc gaaacgctca aacagagtgg ctattgcggg ccgtacctga ttgagatgtg
    11461 gagcgaaacg gcggaagacc cggcggcaga agtggtgaaa gcgcgcatgg ccaaagcagg
    11521 catggtggag gcggcataat gcaaaagcta aaacagcagg tgtttgaagc caacatggat
    11581 ctgccgcgct atgggctggt gacctttact tggggcaacg tcagcgctat cgaccgcgaa
    11641 cgcgggctgg tggtgatcaa gcccagcggc gttgcctacg aaaccatgaa agcagacgat
    11701 atggtggtgg tcgatatgag cggcaaggtg gtggaagggg agtatcgccc atcttccgac
    11761 actgcgacgc atctcgaact ctaccgtcgt tacccgtcgc ttggcggcat tgtccatacc
    11821 cactccactc atgccaccgc atgggcgcag gcggggctgg cgatcccggc attaggcacc
    11881 acgcacgctg actacttctt tggtgatatc ccgtgtacgc gtggattaag cgaagaagag
    11941 gtgcagggtg agtatgaact gaacactggc aaagtgatta tcgaaacgct gggcaacgcc
    12001 gagccgctgc atacgctggg aattgtggtg tatcagcacg gaccgttcgc ctgggggaaa
    12061 gatgcccacg acgcggtgca taacgcggta gtgatggaag aagtggcgaa aatggcgtgg
    12121 attgcgcgca gtattaatcc acaactcaat catatcgaca gctacctgat gaataaacac
    12181 ttcatgcgta agcacggtcc taacgcttat tacgggcaga agtaaaacac gcgctgcgga
    12241 aatttccttc ctcgggagat aactggtcta attccgcagc cgtttttcaa aaaaagcccc
    12301 ctgcgaaggg ggcaaagcaa actatggcaa tgtttcgttg gttatacctg gtgctagcga
    12361 taaatatccg cgctggcgtg catattgccg ttactccccg gttcccgcat cagaattacg
    12421 tggtagtacg ttgcgccttg cgcatctgtt tcttcattta atgcctgacg tgcttcgctt
    12481 tcagtggcga aattatgatt gatataaatc acgcctaagc tttgcacatc gtccatattt
    12541 ctggcctggt ggttattaat ttcaatggct gcccatgtat ttgcacttag caaaagcaca
    12601 gccagaaggg ctaaaactcg actgaacata gatacctcct cgacggctga ctttgtgtgc
    12661 tctcctctgt gatgatcttc tgatttaatt ttaatcaatg ataaagaagt tgatggtgac
    12721 catttctgat gcagttgttc aaaaaaacac tgtgacggaa tgtgatgaac ttcaaatcag
    12781 cggtttagag gttatttgcg aaaggggaga tttatttctg ctccgccctt gagtttagcg
    12841 gggtatgtca gtactataac gcgtcatttt tcagccgacc tttaacacgt tccttgcctc
    12901 cccgggattc ggctgaccca gacaggaggc tgaataatcc gtaaggagca attcgatgcg
    12961 tcattacgaa atcgttttta tggtccatcc tgaccagagc gaacaggttc cgggcatgat
    13021 cgagcgctac actgctgcca tcactggtgc agaaggcaag atccaccgtc tggaagactg
    13081 gggccgccgt cagctggctt acccgatcaa caaactgcac aaagcacact acgttctgat
    13141 gaacattgaa gctccgcagg aagtgatcga tgagctggaa actaccttcc gcttcaacga
    13201 tgccgttatc cgcagcatgg ttatgcgtac caagcacgct gttaccgaag catctccgat
    13261 ggttaaagcg aaagacgagc gccgtgagcg tcgcgatgat ttcgcaaacg aaaccgctga
    13321 tgatgctgaa gctggggatt ctgaagagta atttctgatg accaaccgtc tggtgttgtc
    13381 cggcaccgtg tgcaggactc cccttcgaaa ggtcagtcca tcaggaattc ctcactgcca
    13441 gttcgtgctt gagcatcgtt ctgtgcagga ggaagccggc tttcaccggc aggcatggtg
    13501 tcaaatgccc gttattgtta gcggacacga aaaccaggcc attactcaca gtataacggt
    13561 cggcagtcgc ataaccgttc aggggttcat ttcatgccac aaggcaaaga acggactgag
    13621 caaaatggtt ttgcacgccg agcagattga attgatagat tctggagact agccatatgg
    13681 cacgttattt ccgtcgtcgc aagttctgcc gtttcaccgc ggaaggcgtt caagagatcg
    13741 actataaaga tatcgctacg ctgaaaaact acatcaccga aagcggtaag attgtcccaa
    13801 gccgtatcac cggtacccgt gcaaaatacc agcgtcagct ggctcgcgct atcaaacgcg
    13861 ctcgctacct gtccctgctg ccgtacactg atcgccatca gtaatcggtc acggtccatt
    13921 aatacgactt tgagaggata aggtaatgca agttattctg cttgataaag tagcaaacct
    13981 gggtagcctg ggtgatcagg taaacgttaa agcgggctat gctcgtaact tcctggtacc
    14041 gcagggtaaa gctgttccag ctaccaagaa aaacattgaa ttcttcgaag cacgtcgcgc
    14101 tgaactggaa gctaaactgg ctgaagttct ggcagctgct aatgctcgcg ctgagaaaat
    14161 caatgcactg gaaactgtta ccatcgcgtc taaagctggc gacgaaggta aactgttcgg
    14221 ttccatcggt actcgcgaca tcgctgacgc tgtaactgca gctggcgttg aagtggctaa
    14281 gagcgaagtt cgtctgccga acggcgttct gcgtaccact ggcgaacacg aagtgagctt
    14341 ccaggttcac agcgaagtat tcgcgaaagt gatcgtaaac gtagtagctg aataattcgt
    14401 tattcaacga gacgtaaaaa cgccgaccat tggtcggcgt tttgctttct atttttcgtc
    14461 aggcattaat ttcgcaagta gatccattca ccattctctt gtgtccataa gtcaggatgg
    14521 tttgctggcg ttagatgcat acgatcactc cttatgtctc ggtcctgcgg cactcgctcg
    14581 aaacctaaac ccgagtagat tgcggctgaa tttgataagg aacgtagacc aattttcaaa
    14641 tcatggggta aatcagaata cgcgtttagc aatgcagcaa tgatgaatcc gagggcatta
    14701 gaaacgcctg taacagtgcg aagttggtcg atataaagat cgattgttgg tagttcagat
    14761 gaccagcaat taggcatttg ggcaggaagg gttacaacgt atcctatcgt ttggccggta
    14821 tagtctggag tactataacc aggtcccaaa acaacctcgt gtattgtgta tttactgttg
    14881 ggttcgaagg attcgacatc ttgcaataaa tatttcagtt ctgcggttaa cctcgcaccc
    14941 gcgtaagctt tattgtattt ctgggcgtga atattatcta atgtagcttc atggttccat
    15001 ccaaaagaat tatcaccatt cattgttact gaatcgccac gggtttaaca gacacctcag
    15061 agtcatttaa gatggcttaa agagaggtgc ccatgagcgg taagcgttat cccgaagagt
    15121 ttaaaactga agcagtcaaa caggttgttg atcgcggtta ttctgttgcc agcgttgcaa
    15181 cacgtctcga tatcaccacc cacagccttt atgcctggat aaagaagtac ggtccggatt
    15241 cttccactaa taaagaacag tcagatgctc aggccgagat ccgccgtctc cagaaagagc
    15301 tgaaacgggt taccgacgaa cgggacatat taaaaaaagc cgcggcgtac ttcgcaaagc
    15361 tgtccgactg aggtacgcct ttatccgtga caactcctgt tgctggcctg ttcgcctgct
    15421 ctgtcgggtg ctggatgttc atcccagtgg tttttacgcc tggcttcagc agccgcattc
    15481 acaacgccat caggcagacc tgagactgac aggacagatt aaacagttct ggctggaatc
    15541 gggatgcgtc tatggttatc gcaaaatcca tctggatctg cgtgacagcg ggcaacagtg
    15601 cggagtaaac agagtctgga gactgatgaa acgtgtcgga ataaaggctc aggtcggata
    15661 ccgaagcccg cgggcacgta aaggcgaggc cagtatcgtg tcacccaaca ggctccagcg
    15721 acagttcaat ccggatgctc ctgatgagcg ttgggtaacg gacataacct acatcaggac
    15781 ccacgaaggc tggctgtatc ttgccgttgt tgttgatctg ttctcacgca aaattatcgg
    15841 ctggtccatg caatcccgga tgacaaagga cattgtcctg aac
//
LOCUS       DAJTYG010000085        15833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTYG010000085 DAJTYG010000000
VERSION     DAJTYG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.085"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..457)
                     /locus_tag="OO568_002969"
     CDS             complement(<1..457)
                     /locus_tag="OO568_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642246.1"
                     /translation="MKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVG
                     PDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKP
                     KTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLA"
     gene            complement(627..965)
                     /locus_tag="OO568_002970"
     CDS             complement(627..965)
                     /locus_tag="OO568_002970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642247.1"
                     /translation="MSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            complement(1555..2496)
                     /gene="fabY"
                     /locus_tag="OO568_002971"
                     /gene_synonym="yiiD"
     CDS             complement(1555..2496)
                     /gene="fabY"
                     /locus_tag="OO568_002971"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6642248.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(2541..2978)
                     /gene="dtd"
                     /locus_tag="OO568_002972"
     CDS             complement(2541..2978)
                     /gene="dtd"
                     /locus_tag="OO568_002972"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6642249.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2975..3847)
                     /locus_tag="OO568_002973"
     CDS             complement(2975..3847)
                     /locus_tag="OO568_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6642250.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(3841..4440)
                     /gene="yihX"
                     /locus_tag="OO568_002974"
     CDS             complement(3841..4440)
                     /gene="yihX"
                     /locus_tag="OO568_002974"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6642251.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(4539..5324)
                     /locus_tag="OO568_002975"
     CDS             complement(4539..5324)
                     /locus_tag="OO568_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6642252.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(5358..6254)
                     /gene="yihV"
                     /locus_tag="OO568_002976"
     CDS             complement(5358..6254)
                     /gene="yihV"
                     /locus_tag="OO568_002976"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR6642253.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            6422..7318
                     /gene="yihU"
                     /locus_tag="OO568_002977"
     CDS             6422..7318
                     /gene="yihU"
                     /locus_tag="OO568_002977"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6642254.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPVQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            7346..8224
                     /gene="yihT"
                     /locus_tag="OO568_002978"
     CDS             7346..8224
                     /gene="yihT"
                     /locus_tag="OO568_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR6642255.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNGLCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAVIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            8241..9482
                     /gene="yihS"
                     /locus_tag="OO568_002979"
     CDS             8241..9482
                     /gene="yihS"
                     /locus_tag="OO568_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR6642256.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLFDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWDPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            9620..10522
                     /locus_tag="OO568_002980"
     CDS             9620..10522
                     /locus_tag="OO568_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR6642257.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            10721..12757
                     /gene="yihQ"
                     /locus_tag="OO568_002981"
                     /pseudo
     CDS             10721..12757
                     /gene="yihQ"
                     /locus_tag="OO568_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
     gene            12803..14188
                     /locus_tag="OO568_002982"
     CDS             12803..14188
                     /locus_tag="OO568_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6642258.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTLYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            14231..15634
                     /locus_tag="OO568_002983"
     CDS             14231..15634
                     /locus_tag="OO568_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6642259.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIAKLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            15702..>15833
                     /gene="ompL"
                     /locus_tag="OO568_002984"
     CDS             15702..>15833
                     /gene="ompL"
                     /locus_tag="OO568_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR6642260.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     "
BASE COUNT         3905 a         4039 c         3866 g         4023 t
ORIGIN      
        1 aggctaaagg aacatattcc actgactgcg gtaattcctt acatgcctcc ctcatcattg
       61 cggtatgctt tacaaacaac ggtttttcac tgcccaaaaa aactttctgc ttaattgcac
      121 taagaaaacg caaatcaaaa ataattgttt ttggtttatt attttttggc acatttccta
      181 ttatcccgct aagaaccttc cttgctgcat ggagattttt ttgcacagca cataacacaa
      241 ttgatgaagc cttctccact gcattattga ttttaatttt aaaatccgca tgatatacgt
      301 tgacgggcaa tgcaagtata tctggaccta catcttttgc tgagtattta ttggtgatcg
      361 acggacgtga acccacgaga aaacttagct gcaaattatg ctctatatta tcagctgttt
      421 ttgtggtgtg gttaaacata gagatgccac ctttcattct ttcacacata tcctcaactt
      481 tttcacttcg ttcttgtggc aatttggctt taacattaat ttgatgattt ttacccgtgc
      541 attccttaac tacaagggga atgttatcta aagtgccata caggctaata acgtcaccag
      601 gatttttatc atgtaagtaa gtttgattaa tacttgaagc attcacaaag agggtcaata
      661 ttataactgt ttataacttc acctacatca agtgtgtagt gcttactctg gagactgcca
      721 tccttattta attgtatatc aatcaatata tccccggact ttgtcaccat atctttgcca
      781 taaaccattt gcatagccat ttcgaggaga tttttatcta actgattgcg ggcgttgagt
      841 aagcgattac caaataattt atatactgtt cccgtcattg ccagcgctgc gctttctggt
      901 actttgcgtt tccacagtga ggtttctcta tttttaaaca ggtcatattc attattactc
      961 gacaaaccat gcttcagttc gttaacacaa tcgcacagtt gctcctcacc ttccatttta
     1021 aggcaaagtg aaaattgatt taatggcgtt tacttcggtg atttattact cattagttgt
     1081 ttttctaaat taacaacaga ataactttct tgtttaatga gattaatatt ggcaaccatt
     1141 atagcctcct tcccacggtt taagagcaaa catacaacat aatgattcat aacatacaga
     1201 gcagtaaatg aatgatactg acaatgtatg ccagcccatt atctttgcat aatgtttgtg
     1261 agctatagat tttaaaaata tttatgaaaa aagtatggca ccagaggggg ttttgactat
     1321 caaaaatcgc tcaaacctga actaagatga tgagttaagc attatctgca ataattgcca
     1381 ctcagctcta caaataatat ttaaatgctc ctgaacagtg atgtagcaag acacgtatgg
     1441 tgccaacgaa gaataaaaat ttataaagtt cttattcaat ataaccggaa tagtcaggga
     1501 ctgatgtaaa tgaccggaat gaatgagaga ggcagcaccg cccctctcca taagctactc
     1561 ttcttcgttc ccgccctctt catacgggcc aaatggcttc gcgggcagaa cgatatacgt
     1621 gccttcaaac actgcacccg gcgtctcgtc gccaaagatt tcgacctgca tctgcacccg
     1681 tgcttttcgt ccgcgcgcca gacggtcgag atcgccgctt aaggcaccaa ggtcggctac
     1741 cgcatggggt ttaccgctaa tcggtttgct gtagcggata tgcgcatccg ccagaataat
     1801 cgttccgccg aggtggcgtt cacgcagcat cagccagata agcccccaac cggtgagcgt
     1861 cgccagcgag aataaactcc cggcaaacag cgtatggtgc ggattctgat tgccggtttc
     1921 cggcatggtg gtgataaatt tttgcccggt atattgttga atacgcacgc ccattttttc
     1981 actaagcggg atatgttcgt accacgcctg ttgcagctgc gcgcaccagt cgccgcgatg
     2041 caaaatatca tccagagtgg cgacgggctt aatcatcaaa aaatggcgaa tcggcgtggt
     2101 ggttggcgtg gtgatttctc cctgattaac aaaccccagc ttggcgaaaa actccaccgc
     2161 gtcttcacgg gcgctacagg tcacgcgctt aacgccttcc tgacgcgcca ccgactccag
     2221 ggtcatcgcc atcagcgttc ctaacccttt gtcctgcacg tccgggtgaa cggccataaa
     2281 gcgaatggac gcttcattgt cggcattaat atacaatcgg cctaccgcca ccagattacc
     2341 ctgctcgtcg acgaccatct ggtgatgcgc catcgcatcc cacgcgtcgc gttccgaacc
     2401 ttttggttga tgcaggggct tacgcaacat ttcccagcga aactgatagt aacgctctaa
     2461 ctcttcttct gtttgtggaa cctgaaggtg atacatagcg atactctctc ttgtttcccg
     2521 tgacaaccct ggaagctggc tcatacctgc aaccagaatg tcacggggcc atcattgacc
     2581 agcgatacct gcatatcggc agcgaagcgt cctgtttgcg tgttcatctc ttgctggcgg
     2641 cagcgttcga cgaaatagtc atataacgcc tctgcgcgat ccggtgatgc acctttggag
     2701 aaacttgggc gcatcccccg ttcggtatct gcggcgaggg taaactggga aaccaccagt
     2761 acactgccgc ccgcctgttg cacgttgaga ttcatcttgc cttcggcatc gctaaagatg
     2821 cggtagccga gcacacgctc gcacagacgg tttgctttct gttcgtcgtc atccttttcg
     2881 acacccaata acaccaaaag tcccgcgcca atttcgcccg tcacttctcc ctccacggtg
     2941 acgctggcac gggttacgcg ttgaattaat gcaatcatgg ttcgtcgtct tcttcttgtt
     3001 cagctgcttg ttttagtttg cggtattccc cgagagtgac agtaatttcc gcgccaagca
     3061 agacgataca ccatgtccag tagacccaaa caaagagaat ggggatcacc gccagcacac
     3121 cgtaaatgag ctgatatgac gggaacatgg tgatataaag cgcgaaacct ttctttcctg
     3181 cttcgaacag gagtgcggcg acaaacgcgc cgacaatcgc gtcgcggtta ggtacgcgga
     3241 tggtaggaac aatgctgtac agcaaccaga aggagatcca cgacaacagc aacggaaaaa
     3301 tacgcagcac gttgtcgatg acagtattga gatcgctcgc ccagcgcagg gagagcaaat
     3361 aggaactgat cgccagactg gcccctgcca gcagcggccc cagcgttaaa atcatccagt
     3421 acacggcgaa cgagtaaatt tttggtcgcg ctcgtttact gcgccagatg gtattcaacg
     3481 cgctatcgat ggagtacatc aacaataacg cggtgacgat cagcccgcac gccccaacgg
     3541 cggtcatctt gttggaatta gcaacaaatt gttcgatata ccgctgaata acatcgccag
     3601 tagcaggcag aaagttggca aaaataaagt gacgtaactg aatgctgacg tcggaaaaca
     3661 tgggaaaagc ggcgaaaagc gcaaaaacaa cggcaaccag cggcactaat gagagcaacg
     3721 acacataggc aaggttacct gccagggttg tcatgttgtc ctcatcaata cgttgccaga
     3781 gtagttttag ccaggcccat agtggacggg tacggtgcct ggctttgtcc tgaatggttt
     3841 ttagcataac accttcgcga aatagtccgg gatggtggtt ttatctttca ccagaatact
     3901 ggtaatgcct agctgattag ctccttctat attatcggcg ttatcgtcga aaaagaccgt
     3961 atcgctgggt gaaaaacctt ccgcctgcaa aacatgctgg taaattcgtg cttcaggttt
     4021 gcgcatcccc agatcttgcg acagatagat atggtcagca gcatcacgaa tttccgggta
     4081 ttcttccggc cagaaggtgg tatgcaggcg gttggtattg gaaagcacca ccacgcgatg
     4141 cccctgctca cgcagtttat gcatgatggc gatcacttcc gggcgcagcg caacaaacac
     4201 cgcctgccag ccgtgagaga actgctcgta gcttagcggt agagccatct catgacacag
     4261 cgcctctgcg aacgcttcgt cgctaatttc cccacgctca tgctgatgaa acgcctcccc
     4321 catatgaaaa ctcttcttaa gcgatgccag cggaatacgc gttaaatcgc tccaggctcc
     4381 cagcacacgg ttaaagtcga tatcgacaat cacattacct aaatcaaaga tatagagcat
     4441 ttttgcctcc tttcgcgcca caagaaatta actgtagcgg gaaaggatgg gtttggctat
     4501 gtggcaaggt atgaagccag gttaacggtc acaaaaatct acgcgtcttc ctggggaaga
     4561 atgatttcaa tttggctgtc ttgtaagcgt gattttagcg cagcgggcgg cagctcgtcg
     4621 gtaaagagcg cagtgacctg tgccacgtta ccaatttcaa ccgccgccga agcatgatac
     4681 ttagtgtgat cggcgaccag cagaatattt ctcgcgtgcg ccatcatcgt tttcaccacg
     4741 ttagcttcgt ttacatcaaa ctccatcaac gcgccatcgc tctcaatcgc cccaacgctt
     4801 gttaccagat aatcagcacg aaaatcagcc acaaaggacg ccgctgaagg gccaatgatc
     4861 ccgctattat gagagcgcaa cgtaccgccg ggcaccatca cttcaaagcg cgggttgtgg
     4921 taaagaatat gcgccacacg caggctgttg gtgattatcc gcaaatgatt atggttaagt
     4981 aacgcccggg caacatgctc aacagtcgta ccaatggtga taaatattgt tgaaccatca
     5041 ggaatatagt ctgccaccgc ttcggcaatc gctttttttt tctcggtttg cgaaacctca
     5101 cgctgctcga acgccgtatt aacgacgctg gaagcccgac ccgcgccacc gtgatggcgc
     5161 gtaatcaggc cttgctcgct taatttacga atatcccggc ggaccgtctg cgtggagaca
     5221 tccagcagat ttgccagctc atcaatattc atatacccac gctcggcgat cagcatgagg
     5281 agttggtcgt gccgcgggtt accggttagt tcggtaaggc tcatgaaaat tcctcgaaaa
     5341 accatcaccc tggcatttta tacaaaaagt gacaaaaaag atcgggtttg atcacagtca
     5401 gggatcccgg ctcgtccacc gggacgtgtg cattttaacg ccgctacacc gctggcgaag
     5461 cggactgact ccgctaaatc cccacttgtt gccagcgcca ccgccaaagc gccgtgaaaa
     5521 acatcacccg cacctgtggt atctaccaca tcaactttga aggccggttg atgctgacgc
     5581 ccaccatttt ccagccagtc gcagcctgcg ctaccctggg tcacatagac atgaccattt
     5641 gtgagcgttt gtgcctgttt tagcgcactg gccatctctt tcacgcccgt taagcgcgcc
     5701 agacccggtt ctgaaaaggc cgcgtgatcg cttaatgcca ccagctcact gatatcctgc
     5761 ggcgtaatgt ccccgtccag aacggtcatc acacccgcct gacgggccag ggtgaaggct
     5821 tttttagcgc cgtcgtgcca gcgtacatct gccagcacaa catcccactg agagaaatca
     5881 atttcctcca accactctgc gtcaggcagc agatccgggc tggggtagtt aatgattatc
     5941 cgctcgcctt tggtatccac catgatggcg gattgcgaag atttcgcctg gttataccgt
     6001 ttggtgtaac gggtgttaac gccccaggat tccagctctg ctagcaggct gttgcccgcg
     6061 tcgtcatcac ctacgcgacc aataaaatcg acctgcgccc ccagccttgc cgccgcaacc
     6121 gctgccgtcg ccgctggccc gccaccaact tccgtataat ttctcgccac gtatttaccg
     6181 ctctccgtcg gtaacccttc cacgtaatag atgcgatcca tcacggttat acctacacaa
     6241 gcaacacgaa tcatggtcat tccttaaaca ttttgggatg cgttcatttt catttcacca
     6301 attgtttaaa aagtgacggc tgtcaatttt ttgacgataa attacaaatt cgatcaaaaa
     6361 cagacaagca aagcatgtca taaatgacaa aaagtgacat cactgtattc aggagagggt
     6421 tatggcagca atcgcgttta tcggtttagg acaaatgggt tcgccaatgg cgagcaattt
     6481 attgcagcaa gggcatcaac ttcgcgtctt cgatgtgaac gccgaagctg tgcggcatct
     6541 ggtagacaaa ggtgcgactc ccgccgccaa cccggtgcag gccgctaaag atgccgaatt
     6601 tatcattacc atgttgccga atggcgatct ggtgcgcaac gtgttgttcg gtgaaaacgg
     6661 cgtttgcgaa ggcttatcta ccgatgcgct ggtcattgat atgtccacca tccatccgct
     6721 gcaaaccgat aaattgattg ccgatatgca agccaaaggc ttcagcatga tggatgttcc
     6781 ggtaggccgt acttctgcaa atgccattac cggtactctg ttactgctgg ctggcggcac
     6841 cgctgaacaa gttgaacgtg ccacaccgat cctgatggcg atgggcagtg agttgatcaa
     6901 cgctggcggt ccgggcatgg ggatccgcgt taagctcatc aacaactaca tgagcatcgc
     6961 gctcaatgcg ctttcggcag aagccgccgt tttgtgcgaa gccctgaatc ttcccttcga
     7021 tgttgccgtc aaagtgatga gcggtaccgc cgccggtaaa ggtcacttca ccacttcctg
     7081 gccaaacaaa gtcctcagcg gagatctttc tcccgccttc atgatcgatc ttgcccataa
     7141 ggatcttggc atcgcccttg atgtcgccaa ccagctgcat gtgccaatgc cgctgggggc
     7201 cgcctcacgg gaggtttata gccaggcgcg cgcagcgggt cgcggtcgcc aggactggtc
     7261 cgccattctg gaacaggtcc gtgtcagtgc cgggatgact gccaaagtaa aaatgtaacg
     7321 actggataaa ggaataaatg aatgaatgaa taagtacacc atcaacgaca ttacgcgcgc
     7381 atcgggcggt tttgccatgc tggcggtcga tcagcgcgaa gccatgcgca tgatgtttgc
     7441 cgcggctggg gcacccgctc cggtagccga tagcgtttta actgatttca aagttaacgc
     7501 tgcaaaggcc ctctcgcctt atgcctcggc gattctggta gatcaacaat tctgctaccg
     7561 ccaggtggtt gagcaaaacg cgattgccaa aagttgcgcc atgattgtcg ccgccgatga
     7621 gttcattcct ggcaacggta ttccggtcga tagcgtggtt attgaccgca aaatcaatcc
     7681 gctacagatc aaacaagacg gtggcaaagc cttaaaactg ctggtgctgt ggcgtagtga
     7741 tgaagatgcg cagcaacgtc tggatatggt gaaagagttc aacggactgt gccactcaca
     7801 cggtctggta agcatcattg agccagtcgt ccgcccaccg cgtcgtggcg ataaattcga
     7861 tcgcgaacaa gcggtcatcg atgccgccaa agagctgggc gacagtggcg ctgacctcta
     7921 caaagttgaa atgccgcttt atggcaaagg tccgcaacaa gagcttctct gtgcttcaca
     7981 acgtctgaat gaccatatca atatgccatg ggtgatcctc tcttccagtg tcgacgaaaa
     8041 actgttcccg cgtgccgtac gcgtggcaat gacggcgggc gcatcgggat tcctggcagg
     8101 ccgtgcagtc tgggcatcgg tcgtcggttt accagacaac gagctgatgc tccgtgacgt
     8161 ttgcgcaccg aaattacaac aacttggcga tatcgtcgac gaaatgatgg ctaaacgccg
     8221 ctaagatgag gataaaagga atgaaatggt ttaacaccct aagccacaac cgttggctgg
     8281 aacaggaaac cgaccgcatc tttgattttg gtaaaaattc cgtagtgccg actggttttg
     8341 gctggttagg caataaaggg caaatcaaag aagagatggg cacccatctg tggatcacag
     8401 ctcgtatgtt gcacgtttat tccgttgctg cggcgatggg tcgacctggc gcttacgcgt
     8461 tggttgatca cggtattaaa gccatgaacg gcgcactgcg tgataaaaaa tatggcggct
     8521 ggtatgcctg cgtgaatgac gagggcgtgg tggatgcctc caaacagggc tatcaacact
     8581 tctttgctct gctgggtgcc gccagcgccg tcacaacggg tcacccggaa gcgcgcaagc
     8641 tgttcgatta caccattgag attatcgaga aatatttctg gagcgaagaa gagcagatgt
     8701 gcctggaatc ctgggacgaa gccttcagca aaaccgaaga gtaccgcggc ggcaatgcca
     8761 atatgcacgc ggtggaagct ttcttgattg tttatgacgt cactcacgac aaaaaatggc
     8821 tggatcgcgc gattcgcgtg gcttccgtga ttatccacga cgtcgccaga aataatcatt
     8881 atcgcgttaa cgaacacttc gatacccagt gggatccgct gccggattac aacaaagata
     8941 acccggcgca tcgcttccgc gcgttcggag gtacaccagg ccactggatc gaatggggcc
     9001 gtttaatgct gcacatccat gctgccctgg aagcccgttg cgaacaacca ccagcatggc
     9061 tgctagaaga tgccaaaggt ctgtttaacg ccaccgtgcg cgatgcctgg gcacccgatg
     9121 gcgcggacgg gattgtttat accgttgact gggaaggaaa accggtggtc cgcgaacgtg
     9181 tacgttggcc tatcgtcgaa gcaatgggta cggcctacgc gctctacacc gtcactggcg
     9241 atcgccagta tgaaacctgg tatcaaacat ggtgggagta ctgcattaag tacctgatgg
     9301 actatgaaaa tggttcctgg tggcaggagc tggatgcgga caataaggtc ataaccaaag
     9361 tctgggacgg caaacaggat atttatcacc tgctgcattg cctggtgatc ccgcgtatcc
     9421 cgttagcgcc cggcatggct ccagcggttg cagcgggtct gctggatatt aatgcgaaat
     9481 aactgatctg aaaatagggt gcgggttttc ccctcaccct aaccctctcc ccaaaggggc
     9541 gaggggaccg tccggtgcag tttttgggtt tcacatcagc ctcacgggag gctgtatgtc
     9601 gttaattaag gtgccttgta tgcaaatcac caatatgcat tgcagtggac agaccgtttc
     9661 acttgccgcg ggggattatc acgcgaccat tgtgaccgtc ggtgctggtc tggctgaatt
     9721 aacctttcag ggttgtcatt tagtcatccc gcacaaaccc gaagagatgc cgctggcgca
     9781 tctgggtaaa gtgcttattc cgtggccgaa ccggatcgcc aacggctgtt accgttatca
     9841 ggggcaggaa tatcagttac ccattaatga acatagttca aaagccgcta ttcacgggct
     9901 gcttgcctgg cgagactggc aaattagtga attaaccacc accagcgtta ccctgacagc
     9961 ttttctgccg cccagttacg gctatccgtt tatgctggct tcacaagttg tttattcgct
    10021 caacgcacac acgggtcttt ccgttgagat cgcaagccag aatatcggca ctgtcgcagc
    10081 gccttatggc gtgggtattc atccttattt aacctgcaac ttaacatctg ttgatgaata
    10141 tctgttccag cttccggcaa accaggttta tgccgtcgat gaacacgtaa atccgacaac
    10201 gcttcaccac gttgatgagc tggatttaaa tttcacgcag gcgaaaaaaa tagccgcgac
    10261 aaaaatcgac cacaccttta aaaccgctaa tgatttgtgg gaaatgacca ttacccatcc
    10321 acagcaggcg ctttcggtaa gcctttgttc cgatcaaccc tgggtgcagg tttatagcgg
    10381 tgaaaaatta cagcgtcagg gactggcagt tgaacctatg agttgcccgc ctaacgcctt
    10441 taattcaggt atcgacttat tattactgga accaggaaaa ccgcaccgcc tgttttttaa
    10501 tatttacgga cagagaaaat aacttgtatt gattagcagt caaaatcatt gaagaacatt
    10561 tcactcgcta aaggcaacat tatgtatctt caatttatta agttaaaaac agctcagctt
    10621 ctgtaaagtt tttatatatc agttttatga gaatcatttt acataagcct gtaataacag
    10681 gaaggcaaca atctcgttct caactctatg gagattagtt atggatacgc cacgtccaca
    10741 gttattagat tttcaatttc atcagaataa cgacagtttt accctacatt ttcaacaacg
    10801 tcttatttta acccatagca aagataatcc ttgtttatgg attggctcag gtatagcgga
    10861 tatcgatatg ttccgcggta atttcagcat taaagataaa ctacaggaga aaattgcgct
    10921 taccgacgcc atcgtcagcc agtcaccgga tggttggtta attcatttca gccgtggttc
    10981 tgacattagc gccacgctga atatctctgc cgacgatcag ggccgtttat tgctggaact
    11041 acaaaacgac aaccttaacc acaaccgtat ctggctgcgc cttgccgctc aaccagagga
    11101 ccatatctac ggctgcggcg aacagttttc ctacttcgat ctgcgcggca aaccgttccc
    11161 gctatggacc agtgaacaag gcgttggtcg caacaaacaa acctatgtca cctggcaggc
    11221 cgactgcaaa gaaaatgcgg gcggcgacta ttactggact ttcttcccac agcctacgtt
    11281 tgtcagcacg cagaagtatt actgccatgt tgataacagt tgctacatga acttcgactt
    11341 tagcgccccg gaataccatg aactggcgct gtgggaagac aaagctacgc tacgttttga
    11401 atgtgctgac acatacatct ccctgctgga aaaattaact gccctgctgg gacgccagcc
    11461 agaactgccc gactggattt atgacggggt aacgctcggc attcagggcg ggacggaagt
    11521 gtgccagaag aaactggaca ccatgcgtaa cgcgggcgtg aaggttaacg gcatctgggc
    11581 gcaggactgg tctgggatcc gtataacctc ttttggcaag cgcgtgatgt ggaactggaa
    11641 gtggaacagc gaaaactacc cgcaactgga ttcacgcatt aagcagtgga atcaggaggg
    11701 cgtgcagttc ctggcctata tcaacccgta tgttgccagc gataaagatc tctgcgaaga
    11761 agcggcacaa cacggctatc tagcaaaaga tgcctctggc ggtgactatc tggtggagtt
    11821 tggcgagttt tacggcggcg ttgtcgatct cactaatcca gaagcctacg cctggttcaa
    11881 ggaagtgatc aaaaagaaca tgattgaact cggctgcggc ggctggatgg ctgacttcgg
    11941 cgagtatctg cccaccgaca cgtacctgca taacggcgtc agtgccgaaa ttatgcataa
    12001 cgcctagcct gcgctgtggg cgaagtgtaa ctacgaagcc cttgaagaaa caggcaagct
    12061 cggcgagatc ctcttcttta tgcgcgccgg ttctaccggt agccagaaat actccaccat
    12121 gatgtgggcg ggcgaccaga acgtggactg gagtctcgac gatggcctgg cgtccgtagt
    12181 cccggcggcg ctgtcgctgg caatgaccgg acatggtctg catcacagcg acattggcgg
    12241 ttacaccacc ctgtttgaga tgaagcgcag caaagagctg ctgctgcgct ggtgcgattt
    12301 cagcgccttc acgccgatga tgcgcaccca cgaaggtaac cgtcctggcg acaactggca
    12361 gtttgacggc gatgcggaaa ctatcgccca ttttgcccgt atgactaccg ttttcaccac
    12421 cctgaaacct tatctgaaag aggccgtcgc gctgaatgcg aagtccggtc tgccggtcat
    12481 gcgcccgctg ttcctgcatt acgaagacga tgcacatacc tattccctga aatatcagta
    12541 cctgttaggc cgcgacattc tggtcgctcc ggtacacgaa gaaggtcgca gcgactggac
    12601 gctctatctg ccggaggata actgggtaca tgcttggacg ggtgaagcgt tccggggcgg
    12661 ggaagttacc gttaatgcgc ccatcggcaa gccgccggtc ttttatcgcg ccgatagcga
    12721 atgggcggca ctgttcgcgt cgttaaacag catctaatca catccgcccg tggggaaact
    12781 catgggcaaa gggagaacat cgatgagtca catcacaacg gaagatccag caactttacg
    12841 cctgcccttt aaagagaaac tctcttacgg tattggcgac ctggcctcta acatcctgct
    12901 ggatatcggt acgctttatc ttttgaagtt ttataccgac gttctggggc tgccaggcac
    12961 ctatggcggc attatctttt tgatttcaaa attctttact gcgtttaccg atatgggtac
    13021 cggcattatg ttggattccc gacgcaagat cggtccaaaa ggtaagttcc gtccttttat
    13081 tctgtatgcg tcattcccgg tcaccttact ggcgatcgcc aactttgtcg gcacaccgtt
    13141 tgatgtcacc ggtaaaacgg tgatggccac tattctgttt atgctttacg gactgttttt
    13201 cagcatgatg aactgctcct acggcgcaat ggtcccggca atcaccaaaa accccaacga
    13261 acgcgcctca ctggcggcat ggcgtcaggg cggcgctacg ctgggcctgc tgctgtgcac
    13321 ggtgggattc gtgccagtta tgaatcttat cgaaggtaat cagaaacttg gctatatctt
    13381 cgccgccacg ctgttttcac tgttcggcct gctgtttatg tggatctgct actcaggcgt
    13441 gaaagagcgt tatgtcgaaa cccaaccaac caatccggcg caaaagcctg gcctgttgca
    13501 atctttccgc gcaattgccg gtaaccgccc actgttcatt ctgtgtattg ccaacctctg
    13561 caccttaggg gcgtttaacg tcaagctcgc cattcaggtc tattacaccc agtacgtact
    13621 taacgatccc atcctgttgt cgtatatggg atttttcagc atgggctgta ttttcatcgg
    13681 cgtgttcctg atgcctggcg cagtcagacg ttttggtaag aagaaggtct atatcggcgg
    13741 cctgctgatt tgggtgctgg gcgatctgct caactatttc ttcggcggcg gctcggtcag
    13801 ctttgtggcg ttctcctgcc tggcgttctt cggctcagcg tttgttaaca gcctgaactg
    13861 ggcgctggtt tccgacaccg tcgagtacgg cgagtggcgc accggcgtgc gttcggaagg
    13921 cacgctctac accggcttca ccttctttcg caaagtgtct caggcgctgg ctggtttctt
    13981 ccccggctgg atgctgacgc aaattggcta tgtgccgaac gtcgcacagg ctgaccacac
    14041 cattgaaggg ttacgccagt tgatcttcat ctacccaagc gcactggcgg tagtcaccat
    14101 tgtggcgatg ggttgcttct acagcctgaa cgagaagatg tatgtccgca ttgtggaaga
    14161 gatagaagcc cgtaaacgca cggcgtaata ataataaacg acgccctgcg gggcgttata
    14221 aggagtgatt atgtctgacc ataatccact gacattaaaa ctgaatctgc gggaaaaaat
    14281 cgcctatggt atgggcgacg tcggttcgaa tttaatgctc tgcatcggta ctctgtatct
    14341 cctcaaactt tataccgatg aattagggat gcctgcttac tatggcggga ttatctttct
    14401 ggtcgcgaag ttttttaccg cgtttaccga tatgctcacc ggatttttac tcgactcgcg
    14461 gaaaaatatt gggcctaaag gcaaatttcg tccctttatt ttatatgcag cagttccggc
    14521 ggcgttaatt gcaacgctcc agtttatcgc caccaccttt tgtttgccgg ttaaaacgac
    14581 gattgccacc gcgctgttta tgatgtttgg actttcatac agtctgatga actgctcgta
    14641 tggtgcgatg atcccggcaa ttaccaaaaa cccgaatgag cgcgcgcagc tcgcggctta
    14701 ccgccagggt ggtgcaacca tagggctatt gatttgtacc gtggcgttta ttccgttgca
    14761 gtcgcttttt tctgactcaa ccgtcggtta tgcctgtgcg gcacttatgt tctccattgg
    14821 cggctttatt tttatgatgc tgtgctacag aggcgtcaaa gagcattatg tggacacagc
    14881 gccaaccgga cataaagcca gtattctcaa atctttttgc gcgatatttc gtaatccacc
    14941 attgctggtt ttatgcattg ctaaactgtg tactctggcg gcatttaata tcaaactggc
    15001 gattcaggtc tattacaccc agtatgtgct gaatgatatt aatttattgt cgtggatggg
    15061 attcttcagt atgggatgca tcctcgtcgg cgtattactg gtgccagtaa ctgtaaaatg
    15121 ttttggtaaa aaacaggttt atttagctgg catggtgcta tgggcggtgg gtgatatact
    15181 gaattatttc tggggaagta actctttcac tttcgtcatg ttctcttgtg tcgccttttt
    15241 tggcacggcc tttgtaaaca gcctgaactg ggcactggta ccagataccg tagattacgg
    15301 tgaatggaaa accggtattc gtgccgaagg ttctgtttat accggttata ccttctttcg
    15361 taaaatttct gccgcacttg ctggcttctt gccaggcatt atgctgacgc aaattggtta
    15421 tgttcccaac atcgcgcaaa gcgatgcgac attacagggt ttgcgtcagc tcatttttat
    15481 ctggccttgt gcactggcaa ttattgctgc attgacgatg ggattctttt acacactcaa
    15541 tgaaaaacgg tttgcattga ttattgagga aatcaaccaa cgcaaaaata aagagatggc
    15601 gacagaaaaa aaaacggctt ccgtaacgtt ataaatgtca atagccgcta tttccatcct
    15661 ggtgggtggc ggcctcccta cgtttaaaaa atggacttat tatgaaaaag attaatgcaa
    15721 taattttatt atcttctctg acctctgcct cggtatttgc tggcgcatac gtcgaaaaca
    15781 gggaggcata taatcttgcc tccgatcagg gcgaggttat gctacgtgtg ggt
//
LOCUS       DAJTYG010000086        15714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTYG010000086 DAJTYG010000000
VERSION     DAJTYG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.086"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(14..1009)
                     /gene="yjfF"
                     /locus_tag="OO568_002985"
     CDS             complement(14..1009)
                     /gene="yjfF"
                     /locus_tag="OO568_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR6642261.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(996..2018)
                     /locus_tag="OO568_002986"
     CDS             complement(996..2018)
                     /locus_tag="OO568_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6642262.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADICGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2032..3534)
                     /locus_tag="OO568_002987"
     CDS             complement(2032..3534)
                     /locus_tag="OO568_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6642263.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLGGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3674..4631)
                     /gene="ytfQ"
                     /locus_tag="OO568_002988"
                     /pseudo
     CDS             complement(3674..4631)
                     /gene="ytfQ"
                     /locus_tag="OO568_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4941..5471
                     /gene="ppa"
                     /locus_tag="OO568_002989"
     CDS             4941..5471
                     /gene="ppa"
                     /locus_tag="OO568_002989"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6642264.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5851..6192)
                     /locus_tag="OO568_002990"
     CDS             complement(5851..6192)
                     /locus_tag="OO568_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6642265.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="OO568_002991"
     CDS             complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="OO568_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6642266.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGIYTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="OO568_002992"
     CDS             complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="OO568_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6642267.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGGGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11910..12548
                     /gene="msrA"
                     /locus_tag="OO568_002993"
     CDS             11910..12548
                     /gene="msrA"
                     /locus_tag="OO568_002993"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6642268.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGLYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12871..14214
                     /locus_tag="OO568_002994"
     CDS             12871..14214
                     /locus_tag="OO568_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6642269.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14276..14482)
                     /locus_tag="OO568_002995"
     CDS             complement(14276..14482)
                     /locus_tag="OO568_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6642270.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14807..15361
                     /locus_tag="OO568_002996"
     CDS             14807..15361
                     /locus_tag="OO568_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6642271.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15424..>15714)
                     /locus_tag="OO568_002997"
     CDS             complement(15424..>15714)
                     /locus_tag="OO568_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642272.1"
                     /translation="LGFALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVEL
                     AEQVDAIVSANLAADSNITKEKICEKYKSIIEAFKKEQLALPVLVRCKNSET"
BASE COUNT         3726 a         4318 c         3891 g         3779 t
ORIGIN      
        1 cggagaggca tttttatcgc tgcgcaatgt tgactcttgt cactggcgaa ctctgacgat
       61 tctcccacag caccgttaac ccacgctgta atgctataaa aataaacaac aaaataccga
      121 tggcgatttt cgtccaccag gaactcagcg tgccgtcaaa gttgatgtaa gtctggatca
      181 gtccctgaat tgccacgcca aaaagcgtcc caagcaccgt cccgacgcca ccacttaaca
      241 acgtaccgcc aatcaccacc gacgcgatgg cgtccagttc cacacctacg cccgccagtg
      301 catatccggc ttgggtataa atcgagaaga caatccccgc cagtgttgcc agtccggtgg
      361 agagcatata aatgcgaata gtggtgctgc gagtagaaat ccccatcagg ttcgccgacg
      421 tcgcgttgcc gccaatagcg tacacctgat tgccaaaacg ggtacgatgc gcaaggaaaa
      481 taccgataac caccaccgcc agcatcaaca gccccatcgc actaaggcga ccgccgccgg
      541 ggattttcca cgcaaggctt gagagcgtgt cataaatggg atggtttatc ggaatcgact
      601 cttccgaaac gagatagctg acgccgcgca aaaagaacat ccccgcaagc gtaatgataa
      661 atgccgggat cttcagggcg tcgatcagaa gccccataaa tgcgccgaag gcacagccca
      721 tcaccagcac tagcggaaac gccagcagcg gcgagaggcc gaaatcgcca accacttttg
      781 ccagaaacac gccagtaaag gcgatcaccg aaccgacgga gagatcgata ccgccagaga
      841 ggatcacaaa ggtcatgcca acggcgatta tcccaagaaa ggcgttatcg gtcaggatat
      901 tgcagatcac ccttgtggaa gcaaaaccgg gaaactgggt caggcagtag agatagccca
      961 gcacaaagac gccgatggtg atcatcagcg gcaagttacg ttttatcatg gctacgtact
     1021 cctttaatca gactgataaa gcgttgcgac tgaacaatca gcacacaaag caccaccacc
     1081 gctttcacca cctggttcat ctccggcgga aagcccgaaa gcaaaattcc ggtgttcatc
     1141 ccctgaataa tcagcgcccc cactaccgag agcagcaggt taaagcgccc gcccatcagc
     1201 gatccgccgc caatcaccac cgcgagaatg gcgtccagct ccagccataa tccggcgttg
     1261 ttggcatcgg caccgcaaat atccgccgcc acgataatgc ccgcaatcgc cgcacatagc
     1321 ccgctcaaca cgtaggtgag catgacgatg attcgcgtgt ttaccccggc attttttgcc
     1381 gcccgaatgt tgataccaac gacttcgata aacatcccca gcgccgtttt gcgggtcaac
     1441 agccagaaca ggagaagcgt cagcaccgcg ataatgaccg gcgttggcag gaacaacagc
     1501 gatccgctgc caaaccatga gagatccggc gagttaaacg tgacgatctg cccggcggtg
     1561 atcagttgcg ccacgccgcg cccggcgacc atcaggatca gggtggcgac aaacggctga
     1621 attttgagga tcgctaccag tatgccgttc cacaatcccg ccaggatgcc ggtgcccagg
     1681 gcgcttaaca aaacaatcgg caggctgaat cccgcgaccg tcatcgcagc cgttgtggct
     1741 ccggcgatag ccatcaccgc ccccacagag agatcgatac caccggtggc gatcaccagc
     1801 gtcataccaa tcgccagtaa cgcaacggga gctgcacggt taagaatgtc gatagggcta
     1861 ccgaacaaac gcccatcctg aagcaccacc tgccagaaat gcggggctac caggctatcg
     1921 accagcagca ccagcaacag tgccgccagc tgcggcatcc ccgtcggcca gtgaaaacgc
     1981 ctttttggcg gcgtagtgtc agggagagat tgaggcatca cactgttctc cttacgccgc
     2041 aatggcgttc atgatcgccg gaacggaaag ctgtgccagc gggatctccg ccacctgttt
     2101 gcgatcgcgc atgatgatca cccggtcggc atagcccacc agctcttcca gttcggagga
     2161 gatcaccagc agcgccagac cgtcagcaca cagcgtttca atcaggcgga tgatctcggc
     2221 gtgggcacca acatcaatgc cacgggttag ctcatcaagg atcagaaatt gcggtcgggt
     2281 cagtagccaa cgtgaaagca gcactttttg ctgattgcca ccggagagaa attcaatcgg
     2341 ttgttcagtt gaaggtgtgc gaatgccaag ctggcggata aagcgttcgg caatctcttg
     2401 ctgctctttg cgggaaatgg gtcgtagcca gccgcgctgg gcctggagag caaggatgat
     2461 attttcccgc accgaggcgg cggcgatgat gccatcggtt ttcctgtctt ccgggcagaa
     2521 tccgatgccc agtactgaag cctgatgtgg cgatcgcagg gtttgcggtt tgcctttgat
     2581 caacgccgtg ccgctgtcag caggtttgat accgaagatc acttcggcgg tttcggtacg
     2641 tccggatccc agcaagccag ccagaccgac gatctcgccg gggcgcactt cgagatcaaa
     2701 cggtgctatc gttccttttt tgccgtaatt tttgaacgcg gcaacgggtt tgtcgctcaa
     2761 caatgttcgc ccggcacgct gtagcgcgtg ggtatccagc tcgcgcccca gcatcatttt
     2821 taccagttcg atctgcggta gctcgcgcgt ttcccgacag cctacgaaac tgccgttgcg
     2881 taagacggtg atccgatcgc tgacctgata gacctgatcg agaaagtgag tgacaaagat
     2941 caggctgacg ccgcgatcgc gcaactgacg catcaggcca aataatagct ccacttcctg
     3001 ggtgtcgagg ctggcggtgg gttcatcgag gatcagcact ttggcggaga gatcgatagc
     3061 ccggcaaatg gcgacgattt gctgcatcgc caccgaaaaa cggttaagcg gttcgcgcac
     3121 gtcgagggag aaaccgtaag atgccatcag ttcggtggcg cgcttttcca tctctttacg
     3181 gcgtagaagg ccgaaacgtt tgggttcgcg acctatgaat agattatcag caaccgacat
     3241 attgggtagc aggttgactt cctgatagac ggtgccgata ccgagttgtt gtgcatgggc
     3301 ggtatttttc ggtgagatag tctggcctcc cagccagatg gtgccgcgat cggcgtggta
     3361 tacaccagtt aatgctttga ttagcgttga ttttcccgcc ccgttttcac caagcagcgc
     3421 catgatttcg ccacggcgca ggctgaaatc aacgttgtct aacgctttga cgccggggaa
     3481 aaatttactt aatccttcgg tgcggaggat ctcctggtgt tggtcggtcg tcatggtttc
     3541 ccccggtgta aagcgtgctc ccagtaggca tgataagacg cggtaagcgt cgcatcaggc
     3601 ttcagtgcac catcgccgga tgcggcgcga gcgccttatc cggcctacga atcggcatca
     3661 ggcatagcaa ccctcaatac cccatatttt tcttcttctc taactcttct tttgcggtat
     3721 caggcaggta aagggtggat ttggttaacg tcagcttttc aggcatggtg ccgtcttttt
     3781 tgtatttctc cagcgcatcg aaagcgggtc ccgccatgtt cggcgtcagt tcaacactgg
     3841 cgttcgcttc gccatccatc atcgctttgt agatgtccgg tacaccgtca atggaacccg
     3901 tgaggatatc tttgcccggt ttcaggcccg cttctttaat tgcctgaatt gcaccaatca
     3961 ccatgtcgtc gttatgggcg taaaccatgc agatgttttt gccgttgttt tccgctttga
     4021 taaagctctc catgacttct ttgcctttac tgcgggtgaa gtcacctgac tgcgagcgga
     4081 taattttgat atttggcgca ttcttaatgg cttcggcaaa gcctttctta acggtcaatg
     4141 gcgacgctgg ccccaacggt gccctgcagc tccaccacgt tgcatggttt gccattcact
     4201 tcttttacca gccagtcacc gatcaactta ccttcgagga tgttgtcggc agtaacggtg
     4261 gtcatataga gagatttgtc tttcacatcg atggaacggt cgagcaagaa gaccgggatt
     4321 tcggcatctt tcgcctcttt taataccggt tcccaacccg tcgcaaccac tggagcgata
     4381 aagatcgcat ccaccccttg tgcaacgaag gaacgtaccg ctttaatctg gttttcctgc
     4441 ttttgctgac catcggcaat tttcaacgta attccgcgtt tttcggcctc acttttcgcc
     4501 acattggttt ctgcggcgcg ccagccggat tccgatctga cctgcgaaaa tcctacggtt
     4561 aatggagcgg ccaacgccat agacgacatg gctgccgaga ctgcagagac tacaagtaag
     4621 cgtttccaca tagggttgtc ctcgtcgggg taattattat tggtgaaaag atgttcgcga
     4681 aaaaactata gacaattcgt tatgtaacgg attgcgttac atcacacttc aaaaaagtaa
     4741 ataaaacgtt aatcacaagt ttgtaatcgc tttcatctca ctatgaaaaa tgcggcaacg
     4801 gttatggatt ttcctgctct gtataccgtc ttaaaactgg cgaaaaagga aaatgaagac
     4861 gaaaacaagc aaagacattc ggcgcgagtt ggctataata cttggcactt gtttgccaca
     4921 tatttttaaa ggaaacagac atgagcttac tcaacgtccc tgcgggtaaa gatctgccgg
     4981 aagacatcta cgttgttatt gagatcccgg ctaacgcaga tccgatcaaa tacgaaatcg
     5041 acaaagagag cggcgcactg ttcgttgacc gcttcatgtc caccgcgatg ttctatccgt
     5101 gcaactacgg ttacatcaac cacaccctgt ctctggacgg tgacccggtt gacgtactgg
     5161 tcccgactcc gtacccgctg cagcctggtt ctgtgatccg ttgccgtccg gttggcgttc
     5221 tgaaaatgac cgacgaagcc ggtgaagatg cgaaactggt tgcggttccg cacagcaagc
     5281 tgagcaaaga atacgatcac attaaagacg taaacgatct gcctgaactg ctgaaagcgc
     5341 aaatcgctca cttcttcgag cactacaaag acctcgaaaa aggcaagtgg gtgaaagttg
     5401 aaggttggga aaacgcagaa gccgctaaag ctgaaatcgt tgcttccttc gagcgcgcaa
     5461 agaataaata agttcttcta gcgcaataac cctgaacgcc gggcttcggt tagtaagggt
     5521 ttttttatgc ccgcgataaa taaactctct attccaccat cattattctc agcggttgca
     5581 aggcttgaac ggtaagaaca agcaaacccg accaccattt tgctgttcat agccacttgc
     5641 tggaagttag cagacctcac tcatactcac cgttcaaagc gtgcccccgt tttacccctg
     5701 cacatttgtg aacgccagca gagtttttta tgcgattgca gatatggaaa gcggctcgga
     5761 gagaaaagca aaagatggaa aagttacaaa aaaaaatcgc gagatacatc gcataaaaca
     5821 acgccacccg aaggaggcgt gcttttatta ttacttatcc ctgtctaacc agtcgccgct
     5881 ttcaattagc tttaatccat cgacgggtcg ttgataaacg tacatccatg cactcccata
     5941 cggcgtctga atcaactggc gcgcgtattc accgcccctg gtgcgcaagg catcaagttc
     6001 ggccagcgtg gcgttgtcaa tacgataaac ttcaccgtgt accgttccgt tccccggaac
     6061 tgcgcctgga tagtggccca ggctatacaa ctggtagtta tcgatactga aatcgcccag
     6121 taactgggca ttggtcatcc agtgactgtt gccttgtttg tggcgtaaac tgccgtagac
     6181 aaatattcgc attgctaaaa ctcgaactga tagagcaaat ccagtgcctg gtctacacca
     6241 gacacggctt ccagatatag cttaggcatc aggcgataac gtaacgtgag tgttgctata
     6301 gagtcaaata tacccacgcc gtatttcact tgcagacctg gcaatacata gccgctgacc
     6361 actacctggg aggagtcgcc tactcccagg gtgtcgagcg ctaaattgct tacgccaaac
     6421 gtctcgccga ttttacccac aatctggcca ctttgcgcaa cccccagacc aatcagcatc
     6481 gaggtcattg ccgcactgtc gctctgatcg ctctccagcc cttgtccacg tagcaaataa
     6541 gacaatgcag cttgttgcga catcgccggg tcagagaaga tctccgcttt cggttcgtcc
     6601 gccagaccag tgacgcgaac tccggcgatt acatcgtctt ctgtagcatc cgggttacga
     6661 atagcttcaa tattaagata cggttgatcc ggcggaccag agaacagtaa ctcaccctta
     6721 cgcacaatca gatcctgacc ataggcatgg aagcgccctt cagggatgtt gatctgcccg
     6781 ttcaggccca gcccttgttt gtcctgtacg acgttaagat cgcccgtcag ccgcgctttc
     6841 aggccaaagg cgtcaatgcg cacattgttg ccaacgtgga caatcaggtt actgttaatc
     6901 ggaatcgacg ccgttttcgg ctcttccggt tgcaggttat cgttaagcat caccacatcg
     6961 ctggagacgc ctactgcgct ttccggcaga tcgtgcacca cgatgcgcgc ccacgggaca
     7021 tccacgcgac catcgagggt aaacaggttt ggtgtagcct cgaatacaac atctggcgat
     7081 acatccattc gtaccatcgg cggcacggtg atccgcactt tactgccttt cgccgttact
     7141 cgtgcccgcc agttttcaat ttggctccag tcggcatcac cgttcaggta gatttcaccc
     7201 tgctgggtcc gtactgtacc ggcaagcgtc gagcgcatac cgttaaagtt gaccgcaagc
     7261 tggctcggct gcatatcaaa cggcataaag ttgccgtcga tatccacacc cgtaacctga
     7321 agctgaccaa acaactgcgg gctttgcaca tcaccaccca gacgcaagtt ggcactcacc
     7381 atccccgctg ctttttcccc acgggtaaag atggggttta tcatcgcaag gttgaagtta
     7441 cggatattga cgttgccacc aagattacgg cggccttgcg gatcggtcac ctgcacctgt
     7501 ccatcaaact ggccgttatt ggtcaggcgg atggtccagc ccaattcggc acggttgtta
     7561 cgcaattccg ccgtcagatt cagtgtctga aacgccaccg gcagcgccgc atcgttgacg
     7621 gtttgcgtta cctgcacgtt acgccccgaa agggtgatac tgccctgcgg cagcccctct
     7681 ttcgtggtgt cccaggcaac atccgcttta cccgtgaaga taccgctggc ctgagtggtt
     7741 tctggcataa acggtttcag catggcgagg tcgaagcggt tgagattcac caccgcacgc
     7801 ccttcggccc ccgcatcgat agtttgcggc acgcacagtt ccgcattcgg gttaagccag
     7861 cagtgtggcc cgatgctgat tttttgctcc ttattgcggt aatccagcgc aatatcgcgg
     7921 gtcagcgacc acgggccgac cggcgtctgg aagcgggtat tgctaagagt tcccttccag
     7981 cgttcttctt tgcgatcaaa acttcctgcc agattaagct gcccggagac aggctcgccc
     8041 tgaatccgca actgtagctc gtgctgcttt tcgctgcctt tggcattcag ggtgacgagg
     8101 ttgatattta catccggctg cgaaatttgc tcaacgcgta cgtcgagttt cccggcgatc
     8161 tgatcggtgg atttaatgtc gccttccacg cgaacctgcg ccacggaaag ttcctgccag
     8221 cgcaggccgc gcgcggtgat atctgccagt agttgtggcg cttccaccgt gccgcgtact
     8281 ttcaccagcc ctttcgctgt accgccaagc cccggcagcg cgttatccag ccccggcgcg
     8341 ttgatggtgg catcaagatt gagatctttt acccccagct cgcctttcac ttcggcactg
     8401 tttggcccga gttccagatg aagcccaggg atcatccact gcatataact gttgcctttc
     8461 agcgtgccgt caacgttcac tttgttctgt ttgacgttac cggtcagctt cagttctggc
     8521 acctccatct gccaggtgcc gccgtacagg ctaccgcggg ttttaatcaa gccattgagt
     8581 ttcgacggcc actccgggaa ctctttggcg gtgtaaatgc cattaagcgt tagctcaccg
     8641 cgccaactaa tggcctgctg ccagtcgagc aacgccttga gttcagtttt cccttccagc
     8701 gccgcgacgg tgagtttgtc gagattgacc tgctgttcat tacctttggc atcaagggta
     8761 atggtggctg gcgggatctc ctgtcctttc actgccgtac gcatagagag cgtgtaatcg
     8821 gtcattttgc cggtaagttt cagtttcaga tcatccgcct gatactgctt ctcaccagtg
     8881 aacggccagt aaagctgttt gctgttcact tccacgttga gtggcaatcc ggcttccgcc
     8941 agtcgcgtct gggcgcgtaa atccatatcc accggaccgg aaaggttaac gcctatctcc
     9001 agctgttcgc gcagcgcgcc gcccactttc agcttcacct tttcaccttt caacggctcc
     9061 acgttcagtg tgctgttgag ggtgatatcc accggccagt tgtctgacag ctgcgccgtg
     9121 ccgctggcgt tgacgatccc ctggttggaa tcgatatcca aggcgtccag tttagtattg
     9181 ccgtcaatgc tgctcacttt cagcagcatg gtgcgcacgg tgatgtccgt gtcgcccgtc
     9241 acgcgcagct gctcgccctt aaactcttca atgttcaggt taagcggcaa atgcacgtcg
     9301 gtcatttccg gcaataccgg gcgagaaaag agatctttca gcgtttcgcc gagcggcttt
     9361 tcctccggct gcggattttc aattttcggt tcgaccactt cttcctgcgc cacttccacc
     9421 actttcggca gagcaatcag caggcctttc agcgacgtcg gtttcagggt cagggttttc
     9481 tcctgccagt tcaggccgga ggtgaagtcc atcaccgaca ccgtggtgtc atcaatcttg
     9541 atgttgacgt tgtccagcgc cacccgtgtc agggtgatgg gatacggcgt ggagagatcc
     9601 agcggaccgc tatcttcctc ttcttcaacc tgttcagaag gaggcatttt tttactgtcg
     9661 atgttgacct gaatgtcttt cagcgccagg tcattaatac aaacgctact gttccacagg
     9721 cactcaagac cgaccgccag atgcagatta cccgctttta ccgccacgcc tagctgctca
     9781 taacgaacgt cagacaaggt gagatcgcgc cagccgccgg tgaccttgcc aatatccagt
     9841 cctggcaccc agcgatccgc cgctttaaat acaagatgca ggccgctggt ggtgcccacc
     9901 agaaacgcca ccgatcccag caacagtaag ataacgataa ccacgccgag gctgattttt
     9961 ttccataaac tcataattct ggccccagac cgatgtaaaa ctgtaacccg tgttcgtctt
    10021 tatccgcgac cggtacggca aaatcgagtt tgattggccc gaccggcgat tcccagcgta
    10081 cgccgacccc ggtaccggtt ttaaagtcgc tgcggcgaat atcgcttacc gcttcgccac
    10141 tatcgacaaa caccgcgccc caccattttc cggtcacgtt gtactggtat tccagcgatc
    10201 cggttatcaa cttcgaggcc cctttcaggt caccgttggc gtatttcggg gcgatagatt
    10261 tgtatttgta gccgcgaata ctgcgatcgc ccccgacgaa gaaacgcaga tccggcggta
    10321 ctttgtcgaa atcaccggtt tcaatccagc ccagcgtgcc gcgtgtcaca aagcgatggc
    10381 gatcgtacag tgtgcggatc cagacgttct gcgcctggaa aacggagaaa tcgacatctg
    10441 aaccccaggc cgtgttggag tagtcgatag agtagcgttg cgagtctccc caggttggca
    10501 tcaggccacc acgagaacgc gtgcggctaa tcatcacccc aggataaaac agcatcgtgg
    10561 tgttggtaat ttcaccctgg gtaaagtggt cgaggctcca gcgcaggtta atggcacgct
    10621 gccagccgct ggagagatcc cagtagcgag aagccaccag cgtagtggag tcagattcgg
    10681 tatcgttcag gtcagtgcgc ttaaaaccgc cctgcaccaa ataatattgt tccagtggat
    10741 tcttcagcag cggcatttta tagctgaagt cgagggtctg ttccggcgcg gaaatactgg
    10801 tactggtggt caaactgtga ccgtatgagt tcatccacgg ctttttccac gtcgctttca
    10861 cgcgcggtcc cacgtccgta gagtaaccgc ccccggtttc gatggtgttt tctgttcgcg
    10921 gcgaaaccac gcccgtcaat ggtaatactt tcgtttcgcg cgctttatca aattgtggag
    10981 ctaccaccac cgagttaaac cagccggtag cagaaagtcg gcggttcagt tccgccaaat
    11041 ctttcgattc atactcatcg ccctctttaa acggcaccag attttgcagg tattcatcgc
    11101 ggatttgtga tccttcaaag gtcacatgcc caaagcggta acgttcgcca ctgttataat
    11161 caatatccca gaaggcttta tgcaggccga gcgcaatgcc cagctgcgct ttggtaaatt
    11221 cgctatcgaa ataaccttta cgcaacgcaa tactggttaa ggactttttg aaattttcat
    11281 aatcgccctg gttcagtacc gtaccaatag ccgggcgagt atcgagcaat ttcaaatagt
    11341 ctttatcggt ccgcgcgccg ccgcgcaata ccacatcggt gccgccaatt aacaccggca
    11401 cacctggcgt gactttggcg atcaatacct gccgcccttt ctttggcggt ggacggagat
    11461 caaattcaat ggtcggctgg taataaccca gcgctttcag accttcacgg atggcatcat
    11521 cgacgcgtgc gcgaaagcga cggtctggcg tcacttcatc actttcaatc gtagaaagct
    11581 gcgcacgaac gttcttttcc agctgtcccg ataacccctc gacctgtaga cggacgttcg
    11641 cggcgacggc agatccgctt aagcagagta agcttacaca gcataactgt cggatatagc
    11701 gcacattttc tcctgaatat ccttttttcc tgcccctgga acgccgttaa acggcttaac
    11761 aaaaatccag taatatggat taaaaagcag actaaacccc aaatatttct tatgttttac
    11821 tttagaccta ttcacggtgg ttattgtgtg caaatacgcc tcttgttaca accttaaccc
    11881 caatgaccga ttttcgggag agcgacacca tgagtttatt tgataaaaag catctggttt
    11941 cccccgccga tgccctgcct ggacgtaaca ccccgatgcc cgtagccacg ctgcatgcgg
    12001 tcaacggtca ctcaatgacc aatgtacctg acggaatgga gattgccatt tttgcgatgg
    12061 gttgtttctg gggtgtggag cgtctgttct ggcagttacc cggcctttac agcaccgccg
    12121 caggctatac cggcggctat acgccaaatc cgacttatcg ggaagtgtgc tccggtgata
    12181 cgggtcatgc cgaagcggta cgcattgttt acgatccttc cgtcatcagc tatgagcagt
    12241 tgctacaggt attttgggag aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc
    12301 acggcacgca gtatcgttca gcgatttatc cgctgacccc agaacaggat gccgcagctc
    12361 gcgccagtct ggaacgtttt caggcggcga tgcttgccgc cgatgacgat cgtcacatca
    12421 ccacggaaat cgctaacgcc acaccgtttt attatgccga agatgaccac cagcaatatc
    12481 tgcataaaaa cccatatggt tactgtggaa ttggcggaat tggcgtctgt ctgccgccgg
    12541 aagcatagcg ttacgggtac aaatgtagtt tgttgataaa gtgctctttg tctatgcctg
    12601 atgcggctta aacgacttac caggcctaca aaatcgtcca aattcaatat attgcaagga
    12661 ctgcgtaggc ctgatcggca tagcgcatca ggcagtattg catttatcag cagtctgaat
    12721 tttaaccctc tggcgacttt acagtacctt acgctatact agcgactgaa aatgccggtt
    12781 cactttcttc gaatcggctt tcaatgtgta tttcacacaa attaatcaac tttcccttcc
    12841 gaggatctgg cctgaaaggt cggataagat atgttaaaca gtattttagt catactctgc
    12901 ttgatcgctg taagtgcgtt cttctcgatg tccgagatct cgcttgccgc ctcacgcaaa
    12961 atcaaactta aactgctggc tgatgaaggc aatataaatg cccaacgcgt tctgaatatg
    13021 caggaaaatc ccggcatgtt ctttaccgtg gtccaaatcg gtctgaacgc agtggcgatt
    13081 ctcggcggta tcgtcggtga tgcggcattt tctccagctt ttcacagcct gttctcccgc
    13141 tatatgtcgg cagagctctc tgagcaactg agctttattc tctctttctc gttagtgact
    13201 ggcatgttta tcctgtttgc ggatttaacc ccgaaacgca tcggtatgat tgcgccagaa
    13261 gcggtggctt tgcgtatcat caatccgatg cgcttctgcc tgtacgtttg caccccgctg
    13321 gtgtggttct tcaacggcct ggcgaacatg atcttccgta ttttcaaact gccaatggta
    13381 cgtaaagatg acatcacttc tgatgacatc tacgcggtag tggaagccgg tgcgctggcg
    13441 ggcgtgttac gtaaacagga acacgagctg attgaaaacg tctttgagct ggaatcccgt
    13501 accgttccgt cttcaatgac accgcgtgaa aacgtgattt ggtttgatct ccacgaagat
    13561 gagcaaagcc tgaagaataa ggtggcggaa catccgcact ctaagttcct cgtctgtaat
    13621 gaagatattg accacatcat cggttatgtc gattctaaag acctgctgaa ccgcgtgctg
    13681 gctaaccaaa gcctggcact gaacagcggc gtacaaattc gcaacacgct gattgtgccg
    13741 gatacgttaa ccctttcaga ggcgttggaa agttttaaaa ccgcaggtga agacttcgcg
    13801 gtgatcatga acgagtacgc gctggtggtg gggatcatca ccctcaacga cgtgatgacc
    13861 acgctgatgg gcgatctggt cggtcagggg ctggaagagc agattgtcgc ccgtgatgag
    13921 aactcatggc tgattgacgg cggcacccca attgacgacg tcatgcgcgt gctggatatt
    13981 gacgagttcc cgcagtcggg caactacgaa accatcggcg gctttatgat gtttatgctg
    14041 cgtaagatcc cgaaacgcac cgattcggtg aaattcgccg gctacaaatt tgaagtggtg
    14101 gatatcgata actaccgcat cgaccagctg ctggtgaccc ggatcgacag caaggccacc
    14161 gccctttcgc caaaactgcc tgacgctaaa gataaagaag aaagcgtcgc gtaaccccca
    14221 gaaacatcaa cggctcctga atcaggagcc gtcttattac tgcatagcac tttggttaag
    14281 ccatctctgt ttgcagacgc ataacctgac ggttaacttc ggacatcact gacaaatgca
    14341 gcttatcctt cacttttggg ataagaatct tacccttatc aaattcaaaa gcgccaacgt
    14401 ccttgatgta taaccgtcca cggaacagga tctttacgta cttcgccacc tgaagtgggt
    14461 tgtaccgttg gaaaattttc attgttatct cctgctgagt attacgcctg tgcggtgcca
    14521 caatcggccc aactattatg aggcgcaaat tttaatgctt agtgactata gactatccgg
    14581 gcaatgtttc caccgcgtat aacttttttt acctaaaggt tacaattatt cagaattatc
    14641 tttttaccga agcgcggtct tcagtataag cattcatttt tcatatgatt tgtgcgcttg
    14701 accgcaaact ggcatcacac ttgcgggaaa ttcgataaat agcacatatg attaaaactc
    14761 agacccaagt ggtcggatca cctgcatatc ataagaagga aacaccatga ccctacgcaa
    14821 gattctggca ctcacctgcc tgctgttgcc gatgatggct tccgcacatc agttcgaaat
    14881 cggtcagcga gtgccgccga ttggcattac cgatcggggc gagttggtgc ttgataaaga
    14941 tcagtttagc tacaaaacct ggaacagcgc gcagttagtg ggaaaagtgc gagtactgca
    15001 acatattgct ggtcgcacct ctgcaaaaga gaaaaacgcg acgctgattg aagcgattaa
    15061 atcagcgaag ttaccgcacg atcgttacca gaccaccacc attgttaaca ccgacgacgc
    15121 gattccgggt tccggcatgt ttgtgcgcag cagtctggag agtaataaaa agctttatcc
    15181 ctggtcgcag tttattgtcg atagcaatgg cgtcgcgcgc ggtgcctggc agctggatga
    15241 agaaagttcc gctgtagtgg tgctggataa agacggtcgc gtgcaatggg ccaaagacgg
    15301 ggcgctcaca ccggaagagg tgcagcaagt gatggacctg ctgcaaaagt tgctcaaata
    15361 atggcaataa cataaattct ggaaaaataa ttttccccta tcgttatcta tgggataggg
    15421 gaattaagtc tctgagttct tacaccgaac taatactggt aacgctagtt gctctttttt
    15481 aaacgcctca atgatcgatt tatatttctc gcaaatcttt tctttggtaa tattactatc
    15541 tgcggccaga ttcgcactta ctatcgcatc cacttgttcg gctaattcga cttgttggca
    15601 acctgcatta accagaaaat tataaataag agagtaatct tcatcctcca aaacaacaga
    15661 ggaaccatta cgaaaacgtt catcccctcc atagatttta agcgcaaagc ccaa
//
LOCUS       DAJTYG010000087        15465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTYG010000087 DAJTYG010000000
VERSION     DAJTYG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.087"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..78)
                     /locus_tag="OO568_002998"
     CDS             complement(<1..78)
                     /locus_tag="OO568_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HCR6642273.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLI"
     gene            complement(283..987)
                     /locus_tag="OO568_002999"
     CDS             complement(283..987)
                     /locus_tag="OO568_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6642274.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGLGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="OO568_003000"
     CDS             complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="OO568_003000"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6642275.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="OO568_003001"
     CDS             complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="OO568_003001"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6642276.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFLPPVTGG"
     gene            complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="OO568_003002"
     CDS             complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="OO568_003002"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6642277.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="OO568_003003"
     CDS             complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="OO568_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6642278.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="OO568_003004"
     CDS             complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="OO568_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6642279.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3815..3959)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4224..5132
                     /gene="yvcK"
                     /locus_tag="OO568_003005"
     CDS             4224..5132
                     /gene="yvcK"
                     /locus_tag="OO568_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6642280.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5324..7345)
                     /gene="uvrB"
                     /locus_tag="OO568_003006"
     CDS             complement(5324..7345)
                     /gene="uvrB"
                     /locus_tag="OO568_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6642281.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7924..8601)
                     /gene="bioD"
                     /locus_tag="OO568_003007"
     CDS             complement(7924..8601)
                     /gene="bioD"
                     /locus_tag="OO568_003007"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6642282.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPSSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8594..9349)
                     /gene="bioC"
                     /locus_tag="OO568_003008"
     CDS             complement(8594..9349)
                     /gene="bioC"
                     /locus_tag="OO568_003008"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6642283.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9336..10490)
                     /gene="bioF"
                     /locus_tag="OO568_003009"
     CDS             complement(9336..10490)
                     /gene="bioF"
                     /locus_tag="OO568_003009"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6642284.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10487..11527)
                     /gene="bioB"
                     /locus_tag="OO568_003010"
     CDS             complement(10487..11527)
                     /gene="bioB"
                     /locus_tag="OO568_003010"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6642285.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            11614..12903
                     /gene="bioA"
                     /locus_tag="OO568_003011"
     CDS             11614..12903
                     /gene="bioA"
                     /locus_tag="OO568_003011"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6642286.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12881..13351
                     /locus_tag="OO568_003012"
     CDS             12881..13351
                     /locus_tag="OO568_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6642287.1"
                     /translation="MKHFFANNGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTKSF
                     VVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTGYD
                     GAAPPKGETHRYIFTVHALDVERIDVDEGGSGAMVGFNVHFHSLASASITAMFS"
     gene            13905..14906
                     /locus_tag="OO568_003013"
     CDS             13905..14906
                     /locus_tag="OO568_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6642288.1"
                     /translation="MHKSNCFDTARLVAAMMVLVSHHYALSGQPEPYLFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSILTYFLFGWILNDFSAE
                     YFSHDIVRKTISSIFMSQAPDADITSHLIHAGINGSLWTLPLEFLCYIITGVAVALLK
                     NGKAFIVILLVFVSLSLIGSVSENRDVMFSIPLWLYPLRGLAFFFGATMAMYEKSWNV
                     SNVKITVVSLLAMYAYASYGKGIDYTMTCYILVSFSTIAICTSVGDPLVKGRFDYSYG
                     VYIYAFPVQQVVINTLHMGFYPSMLLSAVTVLFLSHLSWNLVEKRFLTRSSPKLSLD"
     gene            complement(14940..>15465)
                     /locus_tag="OO568_003014"
     CDS             complement(14940..>15465)
                     /locus_tag="OO568_003014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCR6642289.1"
                     /translation="FSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTGDEWAVN
                     IVDINECVSFDKCIFNKPLCYLRSGGVSVTDCYLAGITEVQKPEGRYNTYRGCSGVPS
                     VNGIINVPVAVGATSGSAAIPNPGNLTYRVRSLFGDPASSGDKVSVSVVTINVTRPSP
                     VGVALPSMVEYLAI"
BASE COUNT         3645 a         4092 c         3886 g         3842 t
ORIGIN      
        1 aatgagccat ataccgttta atatcctggc ctcgtgctga aaatctaatt tattgctgtt
       61 ttgcgagtat gattccattc ttttagttgt ccttagaaaa aatattaata ttcaccatca
      121 ataaatttaa aaccacgcat acaaaatact tttgtattta tggatacgga aaacgattcc
      181 gctcgctcat aaggcatatt ttatacctgg tacttgttaa gtgtcgtttt tagtaagaaa
      241 aaaatcccga cacatggccg ggatagtgat ggtgaaaaag aattaacggc ggttgccgaa
      301 gatccgtaac aacatcagga acaggttgat gaagtccaga tacaaggtta acgcgccaag
      361 aatggaatat ttgcgcaggt tcgacgtgtc gcgggtatca atctgctcac ccatattttt
      421 caatttctga gtgtcatacg ccgtcaatcc gacaaagaca atcacgccga tgtaggtaac
      481 tgcccacatc aatgcttcgc ttttcagcca gaagttgacc agcgatgcca gcacaatgcc
      541 gattaacgcc ataaacagca tattgcccaa gccacttaaa tcgcgcttcg tggtgtaacc
      601 gtacaggctc attgcgccga acatcccggc agtaacgacg aaagtactgg cgatagaagc
      661 agcggtatag acaatgaata tactggaaag cgtaagaccc gtcagcgccg aataaagcat
      721 aaagagcatc gtcgttacac ctgcgctcag cttttgaatc atcgctgata acacaataac
      781 caatgctaat tgcgcgatga tcagaccgat taaaaagaca cggttagtga acaacagctc
      841 catcacggcc gcggaattag ccgcatacca ggcaacaaat gcggtcagca acaagccaac
      901 ggtcatccag ccatagactt gcgccatata agtttgcaag ccagcccggg gttgtacgat
      961 tgaatcagaa cgtgggaatc tgtccatgac gatctcctga agatataagg aatatcttaa
     1021 ggatactgca aaatgatgag gctgtgcatc gacgcagcgt aaacgcatgt actgagcggt
     1081 gaaattgccg gacgcagcgg tgccttatcc ggctaacaaa aaactaccag cgttttgccg
     1141 cctgcagatc gctctcccgg gcttcaaccc agcggtcgcc ttccggcgtg gcttcgcgct
     1201 tccagaacgg tgcgcgggtt ttgagataat ccataataaa ctgcccggct tcaaacgcac
     1261 tgctgcgatg cgcactggtg acaccgacaa aaacgatttc atcgcccggc cataattccc
     1321 cgatgcggtg aatcaccgtg acgcgcccca gcggccagcg gttacgcgct tcatcaacaa
     1381 tttctgccag tgctttttca gtcatccccg gatagtgttc gagggttaat gctttgacgc
     1441 tgtcgcccag gttatggttg cgcacctttc cggtgaaggt gactaccgca ccgtcttcgt
     1501 cacgctccgc cagccacggg tactcttctc ctacgctgaa cggctgcggg ccgacaacaa
     1561 ttttggtttc tgccatctta acctccggtt accggcggga ggaaagctac ttcgtcgccg
     1621 tcagtcagcg gatggtcaaa actcaccagc gtctggttga cggcagccag caatttgcca
     1681 tcttccagcg ccagcgccca gcgatcgctc tgcgcagcca ggtgctggcg taacgcttca
     1741 acggttgggt aatccgcagc cacttcggtt gcatctgttc ccaccaactc gcgcacctgg
     1801 gcgaaaaaaa gaactttaat catccgcttc caccttaaag tcacccgact tgccgccgct
     1861 cttcgccagc aaacgtaccg gaccaatcac catatctttt tgcaccgctt tgcacatgtc
     1921 ataaatggtc agcgccgcca cggaggccgc ggttaacgct tccatttcga caccggtttt
     1981 cccggtcagg cggcataagg tttctatacg cacccgattg tgctccggct cggcctgtaa
     2041 attgacttca actttgctga gcatcagcgg gtgacacagc gggatcagat cccaagtgcg
     2101 ttttgccgcc tgaataccgg caatacgcgc agtggcaaat acgtcgcctt tgtggtggcg
     2161 accatcaata atcatcgcca gcgtctcgct gcgcatggtg acaaaggctt ccgcccgcgc
     2221 ttcacgcacg gtttccgctt tggcggagac atccaccatg tgcgcttcgc cagcggcgtt
     2281 gatatgggtc agttgcgaca tacttatttc ttcaaatgtg gatggaaatt acacggacgc
     2341 gtacgggcat ccagctgcgg cgcgatgata ttttcccatg cggtacggca cgctttggtc
     2401 gaacccggca tggcgaaaat cagcgttttg ttagcgacgc ccgctaccgc acgagattgc
     2461 aacgtggaag tgccaatctc ttcaaacgac aacatacgga acacttcacc aaaaccttca
     2521 acctcacggt cgaacaacgg cagcagcgct tcgggagcct gatcaccttc cgtcaggcca
     2581 gtaccacccg taatcaatac cacttgtaca tcgtcgctgg cgatccacgc agatacctga
     2641 gcgcgaatag cgtagcggtt ttctttcaca atggctttat cgacaacgtg atggcccgct
     2701 tcttgcgccg aatcgcgcag atagtgaccg gaggtatcgt cttcttcacc gcgacgatta
     2761 gaaaccgtaa gaatagcaat acgggtcggg ataaattcag tgcttacctg actcatctga
     2821 tctctccttt tgacgtttta gccgccaatg tacgataagt tttgcgtaat accggtgttg
     2881 ttttgatgca ggaaatgggt ctgtttcttc tcccgcagcg ccgctgaaat acgcgcttcc
     2941 agcgcctgtt gctgggtatc gtcttccagc agatcgcgca ggttaacgcc gccttcacca
     3001 aacaggcaga gatggagttt accaatggag gaaacgcgca ggcggttgca agtggcgcag
     3061 aagtcttttt catacggcat gataaggcca atctctccgg cgtaatctgg atggcaaaag
     3121 acttgcgcgg gaccgtcgct gcgttgacgt aattggtgga tccagccgcg acgcagtagc
     3181 tcgtcacgca gaacctgacc agagatgtga tgcttacgga agagctcgct gccctcgccc
     3241 gtttccatca gttcgatgaa acgcagctgg atagggcgat gctggatcca gttcagaaag
     3301 gtgtcgagct ggtgatgatt aacatcacgc atcagcacgg tattgacttt gaccttctca
     3361 aaaccggcct caaatgcagc atcaatccct gccatgacct ggttgaattt atcctgcccg
     3421 gtaatagcgt gaaactggcg ggcgtccaaa ctgtcgacac tgacgttaat gccagtaagt
     3481 cccgcatcgc gccagttcgc cacatcgcgt tccagacggt aaccattggt ggtgaccgca
     3541 atctggcgga tagcgtcgtt ttcccgcaca gcggcgatga tatcggtaaa gtcgcggcgt
     3601 aaagacggct ctcctcctgt caggcgcact ttttccgtgc ccaggctggc gaaggcgcgc
     3661 gtaacccggc gaatttcatc gacggtaaga aagcctttat tggtgacgcc gctcggtttg
     3721 tagccatccg gcaggcagta ggtgcaacga aagttacaca catcggtaat cgacaggcgc
     3781 aagtagtaaa acttacgcgc aaatgcatca gtcagttgtg aagccatgta cacctttcca
     3841 gatacgggag gcgaagtcat ttcttccttc gccctggtgg caatctttac gagcattgtc
     3901 acggccaaag caccgtatca gttgacccag gtgcagaggc tagagtgttt agtggttaag
     3961 ccgatactag cgtgtaaatg agatttttac catccacact ttcgctatgc aatcatgtat
     4021 atagcgtcat gatcgtgcta tttcgctaca gaataaggga aaatggcggt tttactttga
     4081 tatacatcat cagggaggca tagcgtgcat cgtcctttag agatagaaga aaatagtttg
     4141 tatcctcaat attggcaggt taattgctgt ttccccgcaa tttgcgctac tgtagcgcgg
     4201 ataatctgac tccaggaaac tatatgcgca atcgtacgct ggctgatctt gatcgtgtcg
     4261 ttgctctcgg cggagggcat ggactgggac gcgttctctc atcactttcg tctttgggtt
     4321 ctcgtttaac gggtatcgtc accaccacgg ataatggtgg ctcgacgggg cgtattcgcc
     4381 gttcagaagg cggcattgct tggggcgata tgcgcaactg cctcaaccag ctgataacgg
     4441 aaccgagcgt cgcctccgcg atgtttgaat accgttttgg cggcaatggc gaactttccg
     4501 gtcataatct cggaaacttg atgttaaagg cgctggatca ccttagcgtg cggcctctgg
     4561 aagccatcaa tttaattcgt aatctgctga aagtggatac gcatttgatt ccaatgtcag
     4621 agcatcctgt tgatctgatg gcgattgacg atcaggggca tgaagtttac ggcgaggtca
     4681 atatcgacca gttaactacg ccgattcaag agttattgtt aacgcctaat gtacccgcaa
     4741 cgcgtgaggc ggttcacgct atcaatgaag cggatctcat cattattggg cctggcagtt
     4801 tttataccag cctgatgcca attctgctgc tgaaggaaat cgcccaggca ttacgccgca
     4861 cgccagcgcc gatggtttat atcggcaatc tggggcgtga gttgagttta cctgcggcta
     4921 atttgaagct ggaaagcaag ctggcaatta tggagcagta tgttggtaaa aaggtcattg
     4981 atgcggtcat cgtcgggcca aaagtggatg tctcggcggt gaaagagcgg attgtgatcc
     5041 aggaggtact ggaggccagc gatattccgt atcgtcatga ccgccagttg ttacataacg
     5101 cgctggaaaa ggcgttacag gctttaggtt aacggaaatc cgaaggaaaa ttccggcttc
     5161 ctattgaaga caaagtgcgc gttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc
     5221 tacaaaccgc gcaaattcaa tatattgcgg agaaaatgta ggcctgataa gcgtagcgca
     5281 tcaggctgtt ttccgtttgt catcagtctt cttcgctatc ctgttacgat gcggcaataa
     5341 acaactcacg caactgatgc aactggtcac gaatttgtgc cgcttcttcg aactccagat
     5401 tctgcgcgtg ttgcatcatc agcccttcca gttcatggat tttctgctgc aacgctttcg
     5461 gcgacatatc catcggcaca ttatccggct caacaatcgg gcgcgatttt cctctgccct
     5521 tcgctttggt tttggcaatg ttctgcccca gcgccaggat atctaccact ttcttgttca
     5581 agccttgcgg cgtaatgccg tgttcctcgt tgtacttctg ctgtttctca cggcgacgtt
     5641 cggtttcgcc aatcgctttc gccattgatg gagtgatctt atcgccgtag agaatcgctt
     5701 taccgttaac gttacgtgcc gcacgaccaa tggtctggat caacgaacgt tcggaacgca
     5761 ggaagccttc tttgtcagcg tcgaggatcg ccaccagcga cacttccggc atatccagac
     5821 cttcacgcag taagttgatc cctaccaata cgtcgaactc acccagacgc aagtcgcgga
     5881 taatctccat acgctcgacg gtgtcgatat ctgagtgaag ataacgcacg cgctcaccgt
     5941 gttcttcgag atattcggta agatcttccg ccattcgctt ggtcagtgtg gtgaccagca
     6001 cgcgttcgtt aattgccgct cgctgacgaa tctccgaaag aagatcatca acctgtgtcg
     6061 ccaccggccg cacttcgata atcgggtcaa gcaatccggt tggacgcacc acctgatcca
     6121 ccacatcgcc gccggatttt tccagctcgt aattacccgg cgtcgccgaa acatagatgg
     6181 tttgcggcgc taatgcttcg aactcttcaa acttaagcgg acggttatcc agcgctgatg
     6241 gcaggcggaa gccgtactcc accagtgtct ctttacgcgc ccggtcaccg cgatacatgc
     6301 cgccaatttg tggaatggtg acgtgagatt catcgacgac cagcagccca tcggcaggca
     6361 ggtaatcaaa cagcgtcggc ggtggctcac ccggtccacg accggagagg aagcgcgagt
     6421 agttttcaat ccccgaacag tagcccagct cgttcatcat ctccagatca aactgggtac
     6481 gctgggtcag ccgctgctct tccagcagtt tgttgttttc caacagcact ttgcgtctgg
     6541 cggccagttc ttctttgatc tcctccatcg cctgtacgat gcgctcgcgc ggtgtgacgt
     6601 agtgcgtttt cgggtagatg gtaaaacgtg gaatagtgga aacaatctgc ccggtcagcg
     6661 ggtcaaataa cgacaatcgt tccacttcct cgtcaaacag ttccacgcga agtgcaatgt
     6721 catccgattc tgccgggaag atatctatca cctcgccacg aacgcggaaa gtaccacgct
     6781 ggaatgcttg atcattacga gcgtattgca gctccgccag tcggcgcaga atcgcgcgct
     6841 gatcgataat cataccgacc gtgagatgga gcatcatctt gagatataaa tcaggatcgc
     6901 ccagaccata aatcgcggaa acagacgcca ctacaatcac atcacgccgc tccagcatcg
     6961 ctttggtggc ggacaaacgc atctgctcaa tatgttcgtt aaccgaggca tctttctcaa
     7021 tgaaggtgtc ggaactcggt acataggctt ccggctgata gtagtcgtag taggagacga
     7081 aatactccac cgcgttttcc gggaagaact ctttcatttc gccatacagc tgggccgcca
     7141 gcgttttgtt gggcgcaagt accatggttg ggcgctgaag gtcagcaatg acattggcaa
     7201 tggtgaaggt tttcccggag ccagtcacgc caagtaacgt ctggtgcgcc aggccatctt
     7261 ccagcccctc ttcgagacgt cgaatcgcct ctggctgatc gccagaaggt ttaaaagcgg
     7321 aattcagttt gaacggttta ctcatgagtc gctacctgaa ggagttgggc gggcaggtat
     7381 gtaattttac tcgtcgtact taattatgcc aacaaattat actggataaa aaaacagttc
     7441 atcaccataa tatttctgat acagcgtaaa ctccgctgtc aatcatgagc aaaatttact
     7501 ctgtggcgag ataaaactcc ggccttaccg ggttatcccc aaagcaacgg cttttttaac
     7561 atttgtcaag atgagtgatg acagttttat ggcaagagat gcctgtacag tgactgccat
     7621 tgcatttatc taaccagtta aaaaataaaa gatatttctt tgagccgtct ttaacgccaa
     7681 ttcgcgtgca agccgcgtat tctctcgctt gcctcgtgtt ttctaactct aatacacatg
     7741 gttatccaca ggaatagtgg ataactgtct ccagccccta tcccccgccg cctgggcaat
     7801 tcccacattc gccgcatgag gcggaagtaa aatttttttc gtgattattt tagaatttaa
     7861 ttggttaaat tgcagtcaat cgaagacgcg atctcgctcg caatttaacc aaatacagaa
     7921 tggctacaac aaggcaaggt ttatgtactt tccggttgcc gcattttctg gattttctgc
     7981 aagccagggg atctctccca gcagcggcgc gggaatcatg cgggtgagcg tggtcatata
     8041 ttcagcgtga cgttttcccg gaggcgtaac atcgttcgcc acccaacccg ccagagtcag
     8101 tccggcgtgt tgtattacct gcgcagtcaa catcgcgtga ttaatacagc cgagtttcac
     8161 accaactacc agtatcaccg gcagttgttc ctgtgttacc caatctgcaa aagtgaaagt
     8221 gtcagaaagc ggcgtaaacc agccgccagc accttccact aacacccagt cagcctgttg
     8281 ttcaagcgcg cgtaatccgg cgctcattac caatgattct atcggcctgc cctcttgggc
     8341 gctgatgatg tgcggcgagg agggttctgc gaaggtgtaa ggatttaccg ttgcgtaatc
     8401 cagctgcagg ctgctgttgt gctgtaacgc cagcgcgtca ctattacgta agccttccgg
     8461 ggtcttttcg ctgccagagg cgaccggttt ataacctgcc gtccgataac ctactgcctt
     8521 cgcggcttgt aaaagtgcac aactggcgac ggttttcccc acttcggtat ccgttccggt
     8581 gacaaaataa cgtttactca cgagcaatca ctcccaaaaa aagatgatac gtcagaggat
     8641 atcgcccctg ttgttgcggc caggccagtt gcaatcgctg caactgcgaa cgcgttaata
     8701 ttcgcgggtc gcgcccttca tgaagatgcg tagcaccgat gcctttcagc gaacgcatgg
     8761 cactgagcgc atcatcaaac catagtgtga tgagctgaat atgatgttga taacgcacgc
     8821 cgttcagcga ctcttcgatt tcatctggcg gtaaaaagcg attagcatgc ggacgctcgt
     8881 ccaccgcctg ccgcgcctga tgcagttcgg gtaacgatcc ctgcaccagc gtggtaaacg
     8941 cgaccacgcc tccggggcgc accacccgat acagctcgcg gagtgccgtg gataaattac
     9001 cgcaccactg caccgcgaga ttgctccatg caagatcgaa cgtcgcagtc gctaacggca
     9061 gggattcgat atctcccgcc agataatgat ctgcggcatc tttatggcgg gcttgcgcaa
     9121 gcattggcgg cgagagatct aacgccgtca cctgcgcgtg acgttcccgc cagtggcggc
     9181 tcatccagcc aggtccacaa cccgcgtcca gtacgtgggt gtatttacgc tgtggaagca
     9241 ttgccagtaa ggcgtcagca ctctggcgct gtagatctgc atgttgctca tagtgtgcgg
     9301 ctgcccgacc aaatgccgct gcaatggctt gtttattaac cgttgccatg cagcacctcc
     9361 agcagacgat cgatatcctg catttcatgc gcagcggtta gcgttaagcg cagtcgcgca
     9421 gtaccagcgg gtacggttgg cgggcgaatc gccgtgaccc agcagccttg ctggcgcagt
     9481 ttttctgcca gttgtaacgc acggctgtta tcaccgacga tcaatggctg gatggcgctg
     9541 catgaatcag ccagcgtaaa cggcaaatcc tgtactccgg cacgaaaacg cgcaatgagt
     9601 gacaccagtt tttcgcgccg tgcatccccc tcatcacggc gaatgaccgc cagcgacgca
     9661 cgtaatgcct gcgcctgagc gggcggcata ctggtgctgt agataaggtg gcgggcgaat
     9721 tgcagcagat aatccgccac cgtacttgag caaagcaccg cagccccact gacgccaaat
     9781 cctttgccaa aagtcactac cagcaattct ggttttacct tttgcagcca gcagctgccg
     9841 cgcccctgct ccccgataac gcccgtgccg tgggcatcat cgaccatcaa ccagccattg
     9901 tgctgttgcg ttacctgctg gatttccgcc agtggcgcgc tatcgccgtc catgctgaac
     9961 acgccttctg tcaccaccag ttgctgcccc ggacaggggg aagcaagcag tcgcgccaga
    10021 tgagcgacat cgttatgagt aaaacggcga agctgcgacg ggcttaaact ggcagcctcc
    10081 agcaatgagg catggctaag ccggtcggca acaatacggt cctctttcgc catcatcgcg
    10141 gcaatgactg cctgattcgc ggcaaaacca gagataaaca gcagcgcccg cgaatagcca
    10201 agccactcgg ccagttcttc ttccagcgcc tgatgcacca cgctataacc gctgacgtga
    10261 ccggagccgc cgctaccgat gccaaattgc tccgccccct gcttccaggc acggataatt
    10321 tgcggatgat ggcttaaacc taaataatcg ttactggaaa agttcagata ctggcgatca
    10381 tccgccacca gccagcgtcc ggctccttgc gccaccggat aacggcgacg cagggcatcg
    10441 gcagcacgcc gcgcatcgag cgccgcgttg attttctcct gccagctcat aatgctgccg
    10501 cgttgtaata ttcgtcggtg tccggggtca tcagcgcctg tccaagacgt tgctgttgtt
    10561 cgttatcccc tgccagcacg gcagtttgct gcggatttag ccccagtttg cggaacagtt
    10621 gcaggtcttt atcttcttcc ggattcggcg tggtcagcag tttgcaaccg tagaaaatcg
    10681 agtttgcgcc tgccataaag cacatcgcct gagtctgttc gttcatctgc tcgcgtccgg
    10741 cagaaaggcg cacgtaagag gtcggcatca tgatccgcgc tacggcaatg gtgcgaataa
    10801 aatcaaaggc atcaacgtca tcgttatcgg caagcggcgt gcctttcacc ttcaccagca
    10861 tgttgattgg tacgctttcc ggcggcgtcg gcaggtttgc cagttgcagc aataatccgg
    10921 cgcgatcttt taccgtttcg cctaagccca caatgccacc tgagcagact ttgatcccgg
    10981 catcgcgcac tttttccagc gtatcgaggc gttcctgata agtgcgtgtg gtgatgatat
    11041 tgccgtaaaa ctccggcgag gtgtccaggt tgtggttgta gtaatccagc ccggcgttcg
    11101 cgaggcgctg cgcctgagat tcactcaacg tgcccagcgt catacacgcc tccagcccca
    11161 tcgcttttac cccctgcacc atttgttcca ggtacggcat atcgcgttcg tggggattct
    11221 tccatgccgc gcccatacag aagcgcgtcg atcctgccgc tttcgctttg cgcgccgact
    11281 ccagcacctg ttcaacttcc atcaaccgct cggcttccag cccggttttg tagcgcgagc
    11341 tttgcgggca gtatttgcaa tcttccggac aagctccggt cttaatcgac agcaacgtgc
    11401 tgacctgcac ctgacgagga tcgaaatgct ggcgatgcac ctgctgcgct tcaaacagca
    11461 gatccagcaa cggtttttca aataattctg tgacttgcga caatgtccag cgtgggcggt
    11521 gagccatggg gcttctccaa aacgtgtttt ttgttgttaa ttcggtgtag gcttgtaaac
    11581 ctaaatcttt tcaatttggt ttacaagtcg attatgacaa cggacgatct tgcctttgac
    11641 caacgccata tctggcaccc atacacatcc atgacctccc ctctgccggt ttatccggtg
    11701 gcgagcgccg aaggttgcga gctaattttg tctgacggca gacgcctggt tgacggtatg
    11761 tcgtcctggt gggcggcgat ccacggctac aatcacccgc agcttaatgc ggcgatgaag
    11821 acgcaaattg atgccatgtc gcatgtgatg tttggcggta tcacccatgc gcccgccatc
    11881 gagctatgcc gcaaactggt ggcaatgacg ccgcaaccgc tggagtgcgt ttttctcgcg
    11941 gactccggtt ccgtagcggt ggaagtggcg atgaaaatgg cgttgcagta ctggcaagcc
    12001 aaaggcgaag cgcgccagcg ttttctgacc ttccgcaatg gttatcatgg cgataccttt
    12061 ggcgcgatgt cggtgtgcga tccggacaac tcaatgcaca gcctgtggaa aggctatctg
    12121 ccagaaaact tgtttgctcc cgccccgcaa agccgcatgg atggtgaatg ggatgaacgc
    12181 gatatggttg gttttgcccg cctgatggcg gcgcatcgtc atgaaatcgc agcggtgatc
    12241 attgagccga ttgtccaggg cgcaggcggg atgcgcatgt accatccgga atggttaaaa
    12301 cgaatccgca aaatgtgcga tcgcgaaggt atcttgctga ttgccgacga gatcgccacc
    12361 ggatttggtc gtaccggcaa actgtttgcc tgtgaatatg cagaaatcgc gccggacatt
    12421 ttgtgcctcg gtaaagcctt aaccggcggc acaatgaccc tttccgccac acttaccacg
    12481 cgcgaggttg cagaaaccat cagtaacggc gaagccggct gctttatgca tgggccaact
    12541 tttatgggca atccgctggc ctgcgcggca gcaaacgcca gcctggcgat tatcgaatcc
    12601 ggcgaatggc agcagcaggt ggcggctatt gaagtgcagc tgcgcgagca actggcacca
    12661 gcccgtgatg ccgaaatggt tgccgatgtg cgcgtactgg gggcaatcgg tgtggtcgaa
    12721 accactcgtc cggtgaatat ggcggcgctg caaaaattct ttgtcgaaca gggggtatgg
    12781 atccggcctt ttggcaaact gatttacctg atgccgccct atattattct cccgcaacag
    12841 ttgcagcgtc tgaccgcagc ggttaaccgc gcggtacagg atgaaacatt tttttgccaa
    12901 taatggcgat aagttgccgc atcgtcatgt ctttaacggc atgggttacg atggcgataa
    12961 tatttcaccg catctggcgt gggatgatgt tcctgcggga acgaaaagtt ttgttgtcac
    13021 ctgctacgac ccggatgcgc caaccggctc cggctggtgg cactgggtag ttgttaattt
    13081 acccgctgat acccgcgtat taccgcaagg gtttggctct ggtctggtag caatgccaga
    13141 cggcgttttg cagacgcgta ccgattttgg taaaaccgga tacgatggcg cagcgccgcc
    13201 gaaaggcgaa acccatcgct acatttttac cgttcacgcg ctggatgtag agcgtattga
    13261 tgtcgatgaa ggaggcagcg gcgcgatggt cgggtttaac gttcatttcc actctctggc
    13321 aagcgcatcg attaccgcga tgtttagtta atcactctgc cagatggcgc aatgccatct
    13381 ggtatcactt aaaggtatta aaaacaactt tttgtctttt taccttcccg tttcgctcaa
    13441 gttagtataa aaaagctgaa cgagaaacat aaaacgctgt aaatatcaat atgttatgaa
    13501 agatttagtc taaataatag actgcataat actacaaaac acaacatatc cagtcactat
    13561 gaatcaacta cttagatagt attagtgacc tgaggcagag cattagcgca aggtgatttt
    13621 ttgtcctctt gcgctaattt tttgtcaacg gactgggtta gttgaattta caaccatgct
    13681 aagtatctcg ataagcgcag agaaatgatg cagtggtggg cggattggct tgatgagaag
    13741 gttaggtagg caaatccatg cctgagcaga gccttgccct ctacctgagc ccatgctcat
    13801 tctttggcct caacgcgagc atgacctcac tggctggccc caaaatggac tatgacagaa
    13861 agatctgata tttttcctcg caaaaactta gaggctttat tttaatgcat aagagcaact
    13921 gctttgacac ggcaaggctt gtggcagcta tgatggttct ggtcagtcac cactacgcgc
    13981 tctctgggca gccggagccg tacctgttcg gctttgaaag tgctggcggc attgcagtta
    14041 tcatattctt ttcaatatca ggatatctga tttcaaaatc agctatcagg agtgattcct
    14101 ttattgattt catggctaaa agagcgagaa gaatattccc ggcgctggtc ccttgctcta
    14161 tactgacata ttttttattt ggatggatac ttaacgactt cagcgcagaa tattttagcc
    14221 atgacattgt caggaagacc ataagctcta tttttatgag tcaggctcca gatgctgaca
    14281 ttacatcaca tttaattcat gccggaatta acggcagttt gtggacccta ccgcttgagt
    14341 tcctgtgtta tataataacg ggagtagcag ttgccctact aaagaacgga aaagcattca
    14401 tagttatact gttggttttt gtttctcttt cattaattgg aagtgtgtca gaaaacaggg
    14461 atgtaatgtt ctctattccg ttatggctgt atcctttgcg tggactggcg ttcttttttg
    14521 gtgccactat ggcaatgtat gagaagtcat ggaatgtaag taatgttaaa attactgtag
    14581 tttctttgct tgctatgtac gcctacgcta gttatggcaa aggcattgat tatactatga
    14641 cttgctatat cctggtttca ttctcgacaa tagccatatg cacatccgtt ggcgacccac
    14701 tggttaaggg caggtttgat tactcgtatg gtgtgtacat ttatgcattc ccggttcagc
    14761 aggttgttat aaacacattg cacatgggtt tttacccgtc aatgttgtta agtgccgtga
    14821 ctgttctttt cttgtcccat ctttcatgga atctggttga aaaaagattc ctgacacgaa
    14881 gttcgcctaa attatccctg gattgaacaa taaaaggggc catatggccc ctaaaataat
    14941 cagatggcca gatactcaac cattgaaggc agcgcgactc ctaccgggct tgggcgagta
    15001 acattaattg tcaccacgga aacactaacc ttgtcaccac ttgatgcagg gtcaccaaaa
    15061 aggcttctta ctctgtatgt caggttcccc gggttcggta tagctgcaga tccgcttgtg
    15121 gcacctaccg caactgggac attaatgatc ccatttacag acggtacgcc tgaacagcca
    15181 cgatacgtat tgtatctgcc ttccggtttc tgcacctcgg taatgccagc caaataacag
    15241 tcagtaactg acacgccacc gctacgaaga tagcatagag gcttattgaa tatgcactta
    15301 tcgaaagaaa cacattcatt gatgtcgaca atatttacag cccattcatc accagttaac
    15361 gtacaaccac ttacaaagac gaagatcgcc gactgttcta tcgctatact ttgattgttt
    15421 gcagccgtgt ttttgtttgc agtgttgata tgaacaccac tgaac
//
LOCUS       DAJTYG010000088        14926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTYG010000088 DAJTYG010000000
VERSION     DAJTYG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.088"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            82..363
                     /locus_tag="OO568_003015"
     CDS             82..363
                     /locus_tag="OO568_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642290.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(1160..2620)
                     /gene="phoQ"
                     /locus_tag="OO568_003016"
     CDS             complement(1160..2620)
                     /gene="phoQ"
                     /locus_tag="OO568_003016"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6642291.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2620..3291)
                     /gene="phoP"
                     /locus_tag="OO568_003017"
     CDS             complement(2620..3291)
                     /gene="phoP"
                     /locus_tag="OO568_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6642292.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(3460..4830)
                     /gene="purB"
                     /locus_tag="OO568_003018"
     CDS             complement(3460..4830)
                     /gene="purB"
                     /locus_tag="OO568_003018"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6642293.1"
                     /translation="MELSSLTAVSPVDGRYGDKLSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4834..5475)
                     /gene="hflD"
                     /locus_tag="OO568_003019"
     CDS             complement(4834..5475)
                     /gene="hflD"
                     /locus_tag="OO568_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6642294.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5511..6617)
                     /gene="mnmA"
                     /locus_tag="OO568_003020"
     CDS             complement(5511..6617)
                     /gene="mnmA"
                     /locus_tag="OO568_003020"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6642295.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQRGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6671..7132)
                     /gene="nudJ"
                     /locus_tag="OO568_003021"
     CDS             complement(6671..7132)
                     /gene="nudJ"
                     /locus_tag="OO568_003021"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6642296.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQSQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDIG
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(7142..7795)
                     /gene="rluE"
                     /locus_tag="OO568_003022"
     CDS             complement(7142..7795)
                     /gene="rluE"
                     /locus_tag="OO568_003022"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6642297.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7967..9217
                     /gene="icd"
                     /locus_tag="OO568_003023"
     CDS             7967..9217
                     /gene="icd"
                     /locus_tag="OO568_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6642298.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(9320..9643)
                     /gene="iraM"
                     /locus_tag="OO568_003024"
     CDS             complement(9320..9643)
                     /gene="iraM"
                     /locus_tag="OO568_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6642299.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
     gene            complement(9896..9940)
                     /locus_tag="OO568_003025"
     CDS             complement(9896..9940)
                     /locus_tag="OO568_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642300.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            complement(10176..10286)
                     /locus_tag="OO568_003026"
     CDS             complement(10176..10286)
                     /locus_tag="OO568_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642301.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQAHTIKLIFEM"
     gene            complement(10339..10743)
                     /locus_tag="OO568_003027"
     CDS             complement(10339..10743)
                     /locus_tag="OO568_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCR6642302.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNQLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(10964..11695)
                     /gene="bluR"
                     /locus_tag="OO568_003028"
     CDS             complement(10964..11695)
                     /gene="bluR"
                     /locus_tag="OO568_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCR6642303.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRHYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFETRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            complement(11900..13111)
                     /gene="bluF"
                     /locus_tag="OO568_003029"
     CDS             complement(11900..13111)
                     /gene="bluF"
                     /locus_tag="OO568_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCR6642304.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMTHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            13425..13661
                     /locus_tag="OO568_003030"
     CDS             13425..13661
                     /locus_tag="OO568_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HCR6642305.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSHCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            13704..13976
                     /locus_tag="OO568_003031"
     CDS             13704..13976
                     /locus_tag="OO568_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HCR6642306.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKLAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            14005..14271
                     /gene="ariR"
                     /locus_tag="OO568_003032"
     CDS             14005..14271
                     /gene="ariR"
                     /locus_tag="OO568_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HCR6642307.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            14384..14632
                     /locus_tag="OO568_003033"
     CDS             14384..14632
                     /locus_tag="OO568_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642308.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         4005 a         3403 c         3475 g         4043 t
ORIGIN      
        1 atgaggattt tgcttttgat ataagcgaca gccaccgcca gaaactggaa gaatttagcg
       61 gggtgtaatg aggtcagcag catgacagaa gccgaaataa cgggattaat ccgccgcgtt
      121 gccggaatca gccagcaggc tgacgaacag gccacgcagc cggacagcgt gacagccgaa
      181 aactatgcgc gtgttgttgc tgaggtgatg cgccgtgatg gtatagaact taatggcgtg
      241 gatatgcgca acatacgaac cagagttctt gagttgctgg cctaccgtcg ccgcgtggag
      301 atgtatcggg agaaggagaa aatcacgtac cactggaaga agccggagcg gttacggcgg
      361 taactcactg agattaaagc aaagcgcaaa attgcgctgg ctacatccct cacatctgag
      421 gactgttaac cagcaggccg gaatatattc agaaactacg aaattttcgc agtttaatta
      481 ctcgtcagct taatcgctaa cccgctctga tattttcgga aacctcaatt tgaggaagtc
      541 ggcgaggtaa ctgtttgata ttcccgatag cgcaaaaatg cgcggtcggg cagaatgacc
      601 accacctcaa tcgtgacgga gagtgacaac cagtgacaca ccgtgacaaa aagacggttt
      661 aaccgttcat taaaaatcga cgtgattttg tgctttccgg atggcgtggc caacgtcatt
      721 ttttagcgaa aaattctgat taaggtcact ggctgaacgg tcataatgac cacgcagacc
      781 atcgaggcaa ccatagcgat catcgttacg gttggagctt ttccgccatt ggtggaaaag
      841 gtgcacatct ggatcatcat tacggtgatc aagggtatgt aaaacccacc agcctgatta
      901 aaggttaacc ggaaaaaaaa tccggtagcc ccgttatggg gatccccata tcgacattaa
      961 cgcccctcat gaattggtgc gcttccccct ggaaagatta cccgcctgta tatttcttgt
     1021 gtctatttgt tccacgttgt ttcatacagt gcaccgaacg gtgtagttaa tggtgtagtc
     1081 aatttgcgat ttttggcact ttttcaatga aaattgttta atcgaatcaa tgacttgatg
     1141 tagtggtaaa aggacatatt tattcatctt tcggcgcaga atgctggcga ccaaaaatca
     1201 cctccatccg cgcaccgccc agcatgctct ctccggcgac gattttaccc tcatattgct
     1261 cggtgatttc gcgggctacc gccagcccta caccttgccc agggcgtaaa gtatcaaccc
     1321 gttgaccacg gtcgaaaatg acctctcgct tgcttaatgg aataccgggg ccatcatcct
     1381 cgaccacaat atagagatgc tcgtcggttt gccttgcaga aatttcgaca aactcgaggc
     1441 aatatttaca ggcattatcc agcacgttgc ccatcacctc gacaaaatcg ttctgctcac
     1501 cgacaaagct gatctctggc gaaatatcga gagagatatt gacccctttg cgttgataca
     1561 ctttgttcag cgctgaggtg agattgtcca gcagtggggc gaccggatgc agctcgcggc
     1621 tgagcaatgt cccgccgcgc atactggcac gatgcaggta gtagccaatt tgctgtgaaa
     1681 tgcggctgat ttgctccagc attaccggct cagcatcact gacgctcatc ttttcactac
     1741 gcagagaacg cagcgtactt tgcagcaccg ccagtggcgt tttcagacta tgggtcaggt
     1801 cggtgagcgt cgtacggtat ttgtcgtaac gttcgcgttc actttttaac aatcggttca
     1861 ggtttcgtac cagactggtc agttctcgcg ttgtggctgg attgagcaat tcgcggttat
     1921 gttcttccag ttcgcggact tcttttgcca gggcttcgat ggggcgtaaa ctccaccagg
     1981 cggcgaccca cagcagcggg atcactaaca gcagattggc tgagagcaca tagataaacc
     2041 agctccagac catataggaa ctttttagct ccaccggaat ggtatccacc accacaatgg
     2101 ttaattttgg catccgcgat gttgccgggt agacgtttac cgccaccgag tgggtcatct
     2161 ctgcgtcgtc atcatcttcc cgcacttcct gcaactgttg ctgtatcgaa tgatctccac
     2221 tcagcaagag gctggtatcg ttaacatccg cttcaatttc atgaaaacca ttcgatttca
     2281 gccagtcagg ctggatcatt ttcatcagcc agggcacgtc acgttgcgcc cataaaagct
     2341 gcccgttctc atcataaatt agcgtcatgg tggggctttg cttgtcgata ttttcgggta
     2401 actcgacatg caatttattg ttttcccact gcgcaagggt atagaacaga ttgctctcgc
     2461 cacgtaacag ccgaaacgta gttttatcga aactgacgct ataaccgatc agcgcgacca
     2521 ttccgtaggc aagcgaaagc accagtacta ccgctgccgt tgccaacaga aaacgtaccc
     2581 gcagcgagag cgggaaaaaa agacgcagta attttttcat cagcgcaatt cgaacaggta
     2641 gccctggccg cgaacggtgg taatgacttc ttgcggatat tgtgcctgaa tttttttgcg
     2701 cagacgtccc atcagtacat caatggtatg gctttcccgc agctccgcat ccggatagag
     2761 ttgtagcatt aacgaatctt tgctgaccac tttgccatta ttgcgtatca acgtttccat
     2821 aatggtgtat tcgaacgcgg tcagtttgat cacttcgtca ttaatagata attcacggcg
     2881 agagagatca acctgaaacg gggggagcga aatgacctgt gaagccagac cgctattacg
     2941 ccgcattaat gcctgcattc gcgccatcac ctcttcaata tgaaacggtt tagtcacgta
     3001 atcatcagca ccggcactta atacttcgac tttgtcctgc cagctttcac gggcggttaa
     3061 taccagaatc ggcagtgaaa catcgttgct acgccagcgg cgaatcagtg acagaccgtc
     3121 ctcgtcaggc aatccgagat cgacaatcgc aatatccggt aaatgttcat tgagataata
     3181 atcggcttct ttggcatctt ctgcgtcatc gacctgatga ccagcatcct gaatctgaac
     3241 tttaaggtgg tgacgtaaca acgcattgtc ttcaacaacc agtacgcgca tttttatttc
     3301 tccctgtctt aattattaaa atagtgtaac gcgattatgt gtttatgggg gtaaacatta
     3361 aataaaccag cggggagggg aggtaaagtg aaaaaagaaa aagtggataa tcttaataag
     3421 caggccggac agcatcgcca tccggcactg atttgaggct tatttcagct catcaaccat
     3481 cgtgatagct cgaccaatat agttagccgg cgtcatcgct ttcaggcggg ctttctcttc
     3541 ttctggcaac gccagaccat cgataaactg cttcatgcct tcggcgtcaa cgcgcttacc
     3601 gcgagtaagc tctttcagct tctcgtacgg tttttcgatg ccatagcgac gcataactgt
     3661 ctggattggt tctgccagca cttcccagtt gtgatccagc tcatccagca gatggtcacg
     3721 gttcacttcc agtttgctca cgcctttcag ggtggattga tacgcaatca gcgcataacc
     3781 gatacccacg ccgaggttac gcagcacggt ggagtcggtc aggtcacgct gccagcggga
     3841 aaccggcagt ttgcttgcca gatgctgcaa taccgcgttg gaaaggccca gattcccttc
     3901 ggagttttcg aagtcgatcg ggttaacttt atgcggcatg gtggaagaac caatctcacc
     3961 agcaatggtt ttctgtttga agtggttaag ggcgatataa ccccagacgt cacggtcaaa
     4021 gtcgatcaga atggtgttga agcgcgcaac gcaatcaaac agttcggcaa tgtagtcgtg
     4081 cggttcaatc tgggtagtgt acgggttcca ttgaataccc agcgaggtga cgaactcttc
     4141 gctgaactga tgccagtcaa cttccgggta agcggcgatg tgggcgttat agttaccgac
     4201 cgcgccgttg attttgccga ggatctccac ctggttaagc tggcggtgct ggcgctccat
     4261 acggtaggcg acgtttgcca tctctttacc gatggttgac ggcgtggctg gctgaccgtg
     4321 ggtacgagac agcagcggga tatcgcggta ctgaacggcg agatctttaa tgccatcaat
     4381 cagttgacgc cagtacggca ggatcacttc atcacgcgcg gttttcagca ttaatgcgtg
     4441 ggagaggtta ttgatatctt ccgaagtaca ggcaaagtgg atgaattcag aaaccgcgtg
     4501 cagttccggg atctccgcca ctttttcttt caggaaatac tcaaccgctt taacgtcgtg
     4561 gttagtggta cgctcgatgg ttttgatgcg tgcggcatct tcttcgctga aattggcgac
     4621 aattgcatca aggtaaccga ttgcgtcggc agcaaaagca ggaacttcct tgatcgctgc
     4681 gtgcgcggcc agtttttgca gccaacgtac ttcaacttgt acacggaatt tcagcaaacc
     4741 atattcgctg aaaatcccgc gcagcgcgct gagtttatcg ccgtagcgtc catcgacagg
     4801 ggaaacggcg gtcagtgagg ataattccat agatcacaac tccggggtta aatgagcaag
     4861 aatttgtttt gcctgagtgg tcaggcgatt acgagaaaac atcagttgta gacgtccgcc
     4921 gccgacctgg tgccagagca cggcggcgcg aatgcctgcc agcagggttg cgcgaacttt
     4981 cgcctgcact tgtgggcttt gcagtacagc aggggaaccg gtgacctgaa tgcgcgggcc
     5041 aagcgggctg atcacatcaa catagatagc agccatcgcg ctcatcagcg tttcggactg
     5101 taaatcgaag tgttcgagct ggcgttgcag gccgttgatc cggttgccca gagtgtcgag
     5161 cgcgcctttc gctgaggaga gtttgcgctc aagcaccatc aagctgagtg tgtagcgggt
     5221 taattcggcg tttaagccct ggcaactgct ggcattgagc acgccgagca gggtttccag
     5281 cccgacgcgc aggttggctt cgctaccgcc aaaaaccgcc agcgtcgagc tggggttcat
     5341 atcaataata ctgttgagtg agacgtgtag cgcatcggca tcacaatgcc cctggtgagc
     5401 gagttgttgc accaggcgtg ccgactgaca aataccggcc agggcgaggg tgatgtcata
     5461 gtaattcttt gccacgttca ctgcttcctt gttttaagta aagataataa tcagaccggc
     5521 agcggcagac gctgctcaat aataccgcca ccgaggcaca cttcaccgtt atagaagacg
     5581 gcagactggc ccggcgtcac ggcggcaacc ggctcatcga aaatcacttt aatgcgatcg
     5641 tcgtccagcg ccttgacggt gcacggaatg tcggtctggc gatagcgggt ttttaccgtg
     5701 caacgcatag tgccggtgaa tggttcgcga tcgacccagt gcagctgctg ggcaatcaac
     5761 ccgacagaca tcagccgcgg gtgttcatgg ccctgagcga caaccagaat gttgttttcg
     5821 acgtctttgt ccaccacata ccacggttct tcggtgcctt ctttggtgcc accgataccc
     5881 agacctttac gctgaccgag agtgtgatac atcagcccct ggtgctcacc aatttcatcg
     5941 ccatcgacgg taatgatttt gcccggttgc gccgggagat aacggcccag gaactcgcgg
     6001 aatttacgtt cgccaatgaa gcaaatgccg gtagagtctt ttttcttcgc ggtgaccaga
     6061 ccaagatctt cagcaatctt acgcacctgc ggtttttcca gttcgccgac cgggaacagg
     6121 ctttgcgcaa tctgctcatg gctgagcgta taaaggaagt agctctggtc tttattgctg
     6181 tccagaccac gcagcaggcg gctcttgcca tcgacatcgg cccgacgtac gtaatgaccg
     6241 gtagcgatat aatcggcacc taaatcttcg gcggcaaatt cgaggaaggc tttaaatttg
     6301 atctccttgt tgcacagaat atccggattc ggcgtgcgac cggctttata ttcggcaagg
     6361 aacagttcga agacgttgtc ccagtactcg gcagcaaagt taacggtgtg cagttcaatg
     6421 ccgagcttgt cgcagaccgc ctgggcatca gccagatccg ctgccgctgt gcaatattcc
     6481 tcaccgtcgt cttcttccca gttcttcata aacaggcctt cgacctgata tccccgttgt
     6541 tgcaacagcc aagcagaaac ggaggaatcg acaccgccgg acatgccgac gattactttt
     6601 tttgcggttt cagacattgg atcactcacg acattgaact tcaaggcggc gtattctatc
     6661 acgcagcctt ttagatgaca ccctttgtaa aaggccagtt aaaatcgcca atcatctcca
     6721 gcggataacg ttgcccgctt tgataacaac gaatactttc cgccaccagc ggcgaacgaa
     6781 gatttgacgc ctgtaaaatt tcttcggcgc tgacccaacg gcagcagccg atatcgctgt
     6841 catgaggctg ggtcgggcat atttgctcaa gctcaatggc aaagaggaaa cgcaaaaacg
     6901 gcgttttatc tggcgcaatc cactgatgca tacgaataaa gtgttgcgac tgcgcgctga
     6961 tgccggtttc ttcccacagt tcacgggcgg cggcttccac taaggtttca tcggcttcca
     7021 gatgcccggc aggttggttc cataacgctt taccattaat cgtctcttca acgactaaaa
     7081 atttgccttc tgcgtgcacc acgcaagcaa cggtaacgtg cggtttaaac attttgcatc
     7141 ctcaatctgt cacttctcgc cattcgccat tggcaagatt atccaaagag taatctccca
     7201 tcgcatagcg aatcagtcgc agcgtgggga aaccaacatg ggcggtcatg cggcgcacct
     7261 ggcgattacg tccttcatat aaggtgatct tcagccagct ggtgggaata cttttgcgtt
     7321 cacgaattgg tggattccgc ggccataacc acgcgggttc gtcaaccagc tccgcgcctg
     7381 cgggcagggt agggccatca tttaaggtta cgccattgcg caaggcttca agtgcgtctt
     7441 gtgtgggaat accttccacc tgcacataat agatttttcc ggtgcgttta cccggctggg
     7501 ttaaacgcgc ctgcaacgcg ccgttattgg tcagcaccag caacccttcg ctatcgcggt
     7561 caaggcgacc tgctgcataa acgccctgaa ccgggatgaa ttcttttaat gttttgcgtc
     7621 cggcttcatc ggtgaactgc ggaagaacat cgtagggttt attgaagagg atcacacgcg
     7681 tgggctggtt ttcatgttta cgcctggtag aacgttgcga gctgaatcgc ttaacctggt
     7741 gatttctaaa agaagttttt tgcatggtat tttcagagat tatgaattgc cgcattatag
     7801 cctaataacg cgcatctttc atgacggcaa acaatagggt agtattgaca agtcaattac
     7861 aaatcattaa caaaaaattg ctctaaagca tccgtatcgc aggacgcaaa cgcatatgca
     7921 acgtggtggc agacgagcaa accagtagcg ctcgaaggag aggtgaatgg aaagtaaagt
     7981 agttgttccg gcacaaggca agaagatcac cctgcaaaac ggcaaactca acgttcctga
     8041 aaatccgatt atcccttaca ttgaaggtga tggaatcggt gtagatgtaa ccccagccat
     8101 gctgaaagtg gtcgacgctg cagtcgagaa agcctataaa ggcgagcgta aaatctcctg
     8161 gatggaaatt tacaccggtg aaaaatccac acaggtttat ggtcaggatg tctggctgcc
     8221 tgctgaaacc cttgatctga ttcgtgaata tcgcgttgcc attaaaggcc cgctgaccac
     8281 tccggttggt ggcggtattc gttctctcaa cgttgccctg cgtcaggaac tggatctcta
     8341 catctgcctg cgtccggtac gttactatca gggcactcca agcccggtta aacaccctga
     8401 actgaccgat atggttatct tccgtgaaaa ctcggaagac atttatgcgg gtatcgaatg
     8461 gaaagctgac tctgccgacg ccgagaaagt gattaaattc ctgcgtgaag agatgggcgt
     8521 gaagaaaatt cgcttcccgg aacattgcgg tatcggtatt aagccgtgtt ctgaagaagg
     8581 caccaaacgt ctggttcgtg cagcgatcga atacgcaatt gctaacgatc gtgactctgt
     8641 gaccctggtg cacaaaggca acatcatgaa gttcaccgaa ggcgcgttta aagactgggg
     8701 ctaccagctg gcgcgtgaag aatttggcgg tgaactgatc gacggcggcc cgtggctgaa
     8761 agttaaaaac ccgaataccg gcaaagagat cgtcataaaa gacgtgattg ctgatgcatt
     8821 cctgcaacag atcctgctgc gtccggctga atatgatgtt atcgcctgta tgaacctgaa
     8881 cggtgactac atttctgacg ctctggcagc gcaggttggc ggtatcggta tcgcccctgg
     8941 agcaaacatc ggtgacgaat gcgccctgtt tgaagccacc cacggtactg cgccgaaata
     9001 cgccggtcag gacaaagtaa accctggctc tattattctc tccgctgaga tgatgttacg
     9061 ccatatgggc tggactgaag ccgcagacct gattgttaaa ggtatggaag gcgcgatcaa
     9121 cgccaagacc gtaacctatg acttcgagcg tctgatggaa ggcgctaaac tgctgaaatg
     9181 ttcagagttt ggtgatgcga tcatcaagaa tatgtaatca ctacatgtgt taaatattta
     9241 aacgggcgta taacacgccc gttgttttat ttatgtggat attattaata gcatatcgag
     9301 catatttata taaaacctat tacttgagcc catatgggca tatttttata atacaactct
     9361 tatgcaaaca tttatttgtt atttcgcttt ctcctggagg acactcttga ctgcttttga
     9421 gtaaactcca taaattcttg ttgaatggtg caatgttata aatagtaata ggatattctt
     9481 tttccttaag gataatacca agcttaaccg gtgtaaatat ccttccagga ggaagaaata
     9541 tagtagattg ataccagatg atcattttca tatcacccca tatggctgaa aaagatatgc
     9601 cgcatgtagg ttgaattacc gtgtcaatta ctacccactt catttgttat gtcttatccc
     9661 acggtattta atatagttca tttggatgtt catttcttta ttttgcatat gagtatatta
     9721 ccccttcaaa aaataaatta attaaaacga ttgcttatat aaaacaaaat ttaaatcaag
     9781 gaatctcaat ggatgttaaa caaaatgaga tttagtgaaa acaataaatt attcacctcg
     9841 ttttagattt gtttagctat aatgttatac attcaaatga atgaacatcc tgtaattaaa
     9901 acatagcctt tatgctactt ggtgccaatt tgctaaacat tatggttgcc tttttatata
     9961 acgataataa tgaatataaa catgacatga gaataaggtt tcaatttttg agttatatag
    10021 gaaaggttta acctgttcct ggctaaaata catataactg gatgatgact aaaccaaaac
    10081 acatatgcgt taagtattga aacgggcgta tggcacaccc gttgttttta taaatatatt
    10141 aaccgttata aaataacgta taaaaagtca agtgatcaca tttcaaatat caatttgata
    10201 gtatgggctt ggtgattatt tatgggtagc aataaaagga cagtatttat catccatagg
    10261 gatagtctct gtacttttat tcccattatg ctaatgcctt actgaattat gaagcatttc
    10321 ttaagcatcc aactttagct agattaatgg tttattattt tctacatctt caatatataa
    10381 aagcgtatta tcaatggcgt agtaactgcg tttgttatga ttaacatcag taacccaacg
    10441 gaaaacgccc gcgcctgcta gtgttgaaca gtattcccga aatgtagatt ttccgcaaat
    10501 atgaagcaat gcggcctctt ttattttagc aggattcttg gtcgtactaa cttttaactg
    10561 gttcctggtt cctcttaata acaaaaccgt gtcatcgtga gtaataattc tgatgttatc
    10621 cgtagccaga taataaatgt aatgtgcaat acggtgatgt tttaattctg aataaaacca
    10681 ggagaagttt tgctcttttc tcacttgctc aaacatcttt tgaaaaacaa cgacctgatc
    10741 catcaggata ataacctctt gttagttgtg agactgcgta gtgtgcacga tcggttttac
    10801 cacttcaatc tggtctgtcc tttggctgtg atatgtacag agtgtgatag agggattacc
    10861 tgaattctcc cggtgagcat tttgcaacgg accagctccg gtacaaatgc tgttgtgggt
    10921 tcagattata acattctgtc taaggggcgg gataaaggtg aaattagggg gcatgaaaga
    10981 tgactttata accttgctca ccccaatgtt gtaaaagttc gttttgcctt ctcgttggtg
    11041 ccatgcctgt ccagacaatc aatgtttgcg tcgggaacag ttccgggcgc ggcgattcaa
    11101 tgggatcagc aagaacagaa atgtgccatc ctgacaatga taaacgccat gcttccagcc
    11161 acaatcgtgc tctctcttca acattccacg ccatcagaat ggcttcttta ccgggcttac
    11221 ggcgcgtttc gaaaagcgag gttgctgcgt attcaattaa tgcgccgtca aacatactgc
    11281 tcataatgcg ggaggtgttg tgatcaagca cgagacgctg gcgaacagga aggtaaacat
    11341 gattaatcaa ttgatctact gggtactctc gacccagtga aataattctc gcgcgtagtt
    11401 tggcaggatt agccatacga agaattgaca tcatctcttc ttgcaggcgg ctccagtcat
    11461 cttccgtatc ctggctggtg gtttccagta atgctttaac tttgcctaca gggacgccat
    11521 tactgatcca acgcttgatc tcttcgatgc gttgtatgtc ttcttcatca aagagtcggt
    11581 gtccgccttc actgcgctgt ggttttaaca aaccgtagtg gcgttgccag gcccggagag
    11641 tgacaggatt aatcccgcaa cgttcagcaa catcaccaat gctgtaataa gccacaattc
    11701 ctccttgcgg tcacaaatct ccgtcgcctg tacacgaccc aataatactt tgtacaatat
    11761 acgctaaaat tgtacaaagt ataaataaga tttcagctaa attggatgaa acattatttt
    11821 taatgtagat taaatttaaa cataacgtat tcattttcac gatgatttac tgaaatcatg
    11881 tgaaagaatg tgctgaaaat tattttttct ctggccacgc tatggaaggg ataccattca
    11941 atttagcttt agcaaacaga tctccctgaa acatctcaat tcctgcggat tcaagccaca
    12001 tccactcttc tggtgttgcc acgcccatag cactgacttg aatttcaagt gatgtacagc
    12061 attttatgat cgcttgaata attgcctgcc gtggcccgct tttatgaaca ttggtaatca
    12121 attcctgact gattttaatt ctgtcaggct ggaagcgtga caggagtaac aaaccggcaa
    12181 aacctgcgcc aaaatgatca attgctacac tgataccggc agccttaagc gatttaatcg
    12241 cttctgcaaa ctcatcaaac cgagatataa cttcactttc agtaaattca acgatgattt
    12301 gttcaggcac cagagcattg gcctttattt catcaagtaa aaaagagact gcgtcaggtt
    12361 cgttaaccag ggtcataggt aatagattga ttgaaatcat tttatcaccg agctcaagtg
    12421 cgtgtgtcat cgcgaatgca agcgccttac ttttgagatc cgctgtgtag atttccccgt
    12481 ctttacgctg cccaaccgct atggctgatg ggctatcttc atttttttgc acaatggctt
    12541 caaaagcgat tatccgccgc gataagggat cgacaatagg atgaaaggca aagtggtcgt
    12601 ttatagttgg tgaaagggta caggaatcaa gttctttatc agatccgtca gcgataaaaa
    12661 gccaggagtc ttcggcaggg atctcgaaat aggttgattg ttcggttgca aggacaaaag
    12721 tacgaaaaaa ttgtagcgct ctgtcatcat aagttagctg aaattttgat gtgcctttgt
    12781 cgaatacggc ctgtaaaacg tcatctcgct cgtgcaggcg caaatcaaat aattccattc
    12841 ccgctttgcc aaaacggcga gcaggcgcgt aatcgcacag cagctcaaca atattatagt
    12901 gccgtggatc ttggcatata gcccgatata tcattttaac ctgttcttcc ggaccttcca
    12961 gaagctggaa aaaatgagaa ccattaaaca gtaagatccc tgttacgtca gactgcatgt
    13021 tcctgcgatt tgctatcgaa accatttctt cgattttttt gacaggttcg tcgtcacgta
    13081 tatggctacg ataaataagg gtggtaagca ttaacaatcc agggtaatgg gtgaggcgag
    13141 agtaagacgg taacagacat atcttcttgt gtctttcttt taataccaaa acataaccgt
    13201 ttctttacat tgataaaaaa tggaaaaagt tgaacacgag ttggcgaaaa atcttgtata
    13261 gattgtcagt taaatgatgc aatatgtttt atcataacac attgttttat atacattagt
    13321 actaattgca aaaaattaat ttatcattct gtacacatat ttcgtacaag tttgctattg
    13381 ttacctcact tcacattgat taacattttt aacagaggcg tagcatgcat caaaattcag
    13441 tgacttcaga ttctgcggga gcaatcactc gttactttgc aaaagctaac ttgcctactc
    13501 agcaggaaac tctcggggaa attgtgactg agattttgaa agatggacgt aatctgagtc
    13561 gaaagtcgca ttgtgccaaa cttctttgcc gactggaaca agcgacaggg gaagaggaac
    13621 agaaacatta taatgcacta attgggttgc tttttgaata atgcaacagc ctgattattt
    13681 aacaggttag ctatgaagtc gttatgaaga catctgataa tgaacgtata aaatatgaaa
    13741 ttactggcca ggcggtgctc cagatactgc gcatgaagat aaatttttca ttgcagactc
    13801 ttattaagca attactcgta atgaaattgg ctgaggaaga tgctttccga cgcgatttaa
    13861 ttgacagcat aattcgtgat tttagcaata gtgattcagg agggccaaac cgaagaacag
    13921 cgacagctga caataaaagt atgttcaatg gtaagaaaat aaacagaata cattaaaatt
    13981 tcataagtaa gatgagaggt taccatgctt gaagatacta caattcataa tgcaataact
    14041 gataaagcgt tagcaagtta ctttcgcagt tcgggtaatt tgttagaaga agagtcagca
    14101 gtgttagggc aggctgtcac caatttaatg ctttcaggcg ataatgttaa taataaaaat
    14161 attatcttaa gtctgataca ctccctggaa acaacaagtg atattctcaa agctgatgtg
    14221 attagaaaaa cactggaaat cgtgttgcga tacacagctg atgatatgta acttctaatt
    14281 attaagtata agtttataga aaactcattc atcttatttt tgtctgtcgc tttagactat
    14341 acacagcata attttattgg gttaatattt ctacgaggct gacatgaata attcaatccc
    14401 agagagattt atttttcaat gtgctttgtt taaaaatctc gagagggagg tgttcatgac
    14461 gcatgggtat gttgatagtc atattattga tcaggcttta cgcttgcgtt taaaagatga
    14521 aaccagtgtg attctttctg atctctatct gcaaatattg cagtacattg aaatgcataa
    14581 gactacacta acggatatca ttattaatga cagggaatcc gtgctctctt agaacttgag
    14641 taaggtttta gaataaacat cagctgtatc accatgctga tgcaaagtga gcatttcagg
    14701 cgttatgctt tcttattatg tccgcaatat caggtgtcaa gaatggagag ttctcgctct
    14761 ccattcttga cgcctgatat cccgcctaac ttattttgta ctgcctacat tatgctcaat
    14821 aattaatggg ttagatcaag atggagaaaa ttttatcttc ggcgtggata tttcttatgc
    14881 tctctaccat gcatctctgt agatgccagc gcgagcgaat gaatgg
//
LOCUS       DAJTYG010000089        14924 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTYG010000089 DAJTYG010000000
VERSION     DAJTYG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.089"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            596..793
                     /locus_tag="OO568_003034"
     CDS             596..793
                     /locus_tag="OO568_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642309.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(743..1936)
                     /locus_tag="OO568_003035"
     CDS             complement(743..1936)
                     /locus_tag="OO568_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6642310.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2072..3796
                     /gene="iucA"
                     /locus_tag="OO568_003036"
     CDS             2072..3796
                     /gene="iucA"
                     /locus_tag="OO568_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6642311.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3797..4744
                     /gene="iucB"
                     /locus_tag="OO568_003037"
     CDS             3797..4744
                     /gene="iucB"
                     /locus_tag="OO568_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6642312.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4744..6486
                     /gene="iucC"
                     /locus_tag="OO568_003038"
     CDS             4744..6486
                     /gene="iucC"
                     /locus_tag="OO568_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6642313.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAEVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6483..7799
                     /gene="iucD"
                     /locus_tag="OO568_003039"
     CDS             6483..7799
                     /gene="iucD"
                     /locus_tag="OO568_003039"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6642314.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSPASLTHYTANIQE"
     gene            7805..10000
                     /gene="iutA"
                     /locus_tag="OO568_003040"
     CDS             7805..10000
                     /gene="iutA"
                     /locus_tag="OO568_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6642315.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10551..10694)
                     /locus_tag="OO568_003041"
     CDS             complement(10551..10694)
                     /locus_tag="OO568_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642316.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10944..14831
                     /gene="sat"
                     /locus_tag="OO568_003042"
     CDS             10944..14831
                     /gene="sat"
                     /locus_tag="OO568_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCR6642317.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPEETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSRTSHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         4020 a         3553 c         3707 g         3644 t
ORIGIN      
        1 gagtgaaaca aaggcagctt atcgttgttt ttcaaacgat aaagtctctg ctaataaaat
       61 catggctcct cacaaagaaa atatcactga aagagctcag cagtttaaaa gagttttagt
      121 tttacaggat acaacagaac ttaattattc tggtcagaaa gaaaaacaag gagttggtcc
      181 caaaaaacat aaagatgaaa gaaacttatt tcttcatcct caactcgtca tcagtgagtc
      241 aggtgtttgc ctcggtgttt atgatgacta tcagtggttc agggatgaat taaaaaccag
      301 taaaaatacc cgacaagaaa tctgcaatga tttacttcat aaaaaacatg tcagtgaaaa
      361 agaaacctgg cgttgggttg aaggatataa taaagcaaca gaactggctc gccaatgccc
      421 tgatacccat gtattgtcaa tttctgatcg tgaaggcgat ttttatgacc tgtttgaacg
      481 cgcagcgcag actcccggaa taaaagctga ctggcttgtc agaatgaaat ttaataacag
      541 agcaacgctg aatattaatg gtaaacggga tcataggttg ttgcacgaaa gaatcatgga
      601 gattacaccg cagcaactgg ttgaattcac tatacctgat gggcgggggc agcaagccag
      661 aacactgcct gacatgcttt catcagacgc aggtcctctc tcatatcctc tgatttactc
      721 cagtaaggtg atgatcatat ctttatttga tagtacggta atgccagata aatacagaca
      781 caatggtaaa taatatagtt gcaaataatg ctgccataat cagcgcgatt gcgacacctg
      841 ttttcgatac cagcggcacc agcaaagcgc acagtccata gccaagtgtg tgactggtag
      901 cgagccagcc cgcgcctgcg ccatcagcca gcttatcatt cagtagaagt tgataagcgg
      961 gtgttgccag agctgctcca aatgacagca ctgcacagcc cgggtaaaac aaccatagct
     1021 gttcggagag catgatagcc agaccaccac tcatcaacac tccggcactc aggagcaggg
     1081 ccaccggagt caggcgctgc ggacggagta ccccgaactg cgcgataagc gcagctactg
     1141 cggacagacc caacagccag gccacctgtt ggctaatggc ggtggtatcc gtagcgaact
     1201 ggcgagtaag ggcaggcgaa agtccaagct gcatcatgct caccgccgca gccagtaata
     1261 ttgcgcaaag cagataaggc agacaatccc gcttcagact gacgctcttg cactccggtg
     1321 tgggctgtgg tggtgtgccg ggcaaccgca gcagcatcag cagcgccagc actggggctg
     1381 ccatcagcag ccccagtggc gccagaggat gaatggccag cattgctgcc gcgcacagcg
     1441 gcccgaatag cctgccgcaa ctcaggccgg agctgatggt tgccagagcg gccatgcgat
     1501 tcccttctcc agctctctgc aatgcccaga cctgacaggc tggcaccatt gccgacacgg
     1561 tcagcccgta ggcgatccgg gcgatgatta atattcccag tcccaccgct gttgttatgg
     1621 cgctggtagc catcagcacg cttcccagcc ctagcagggt aaagcttatg ccatatcctc
     1681 ccagagccca caacaccaca ggtttactgc cagtacggga aatttgcttt ccccaccacg
     1741 gggacgatgg cagaaacagc attgagccaa gcatcagcag ggcagcccat acagacagac
     1801 tcagatttgt ctgtatgacc agaacaggga gcacaaccag cagaccgttc tgtccgatac
     1861 cgagaagccc ggcactgaac gcaagtggcc agcaggacag tggtttttgg ggcgtatctt
     1921 cgatccctgg tgacattata atgtttcaac tccatgtatt aattgtgttt atttgtaaaa
     1981 ttaatttatc tgacaataac atttattgtt gataatgaga atcattattg acataattgt
     2041 tattatttta ctgtgtggga gctgtttgac tatgaccctg ccctctgaaa aaccagccac
     2101 agatgtggct gcgcagtgct tcctgaatgc actgattcgt gaaaccacag actggaaact
     2161 gacagaatac ccgccagacg aattgctcat cccgctggat gagcagaaat cgctccattt
     2221 cagagtggct tatttctccc caacccagca tcaccgcttt gcatttcctg cacgtctggt
     2281 cacggcatca ggcagttatc ctgtcgactt taccactctc tcccggctta ttattgataa
     2341 gctacgccat caactgtttc tgcccgttcc cctctgcgaa actttccacc agcgcgtgct
     2401 ggaaagccat gtccatacgc aacaggcaat tgatgcccgt catgactggg ccgctctgcg
     2461 tgaaaaagcg ttgaattttg gcgaggctga gcaggcgctg ctgacaggac acgctttcca
     2521 ccctgcgcct aagtctcatg aaccgtttaa ccggcgggag gctgaacgct acctgcctga
     2581 tatggcaccc cacttcccac tgcggtggtt ttcggtggat aaaacgcaaa tcgccggtga
     2641 aagtttgcat ctcaaccttc aacagcggct gacgcgattt gccgcagaga atgcgcctca
     2701 gttactcaac gaattaagtg acaaccaatg gctgttcccg ttgcacccgt ggcaggggga
     2761 atatcttttg cagcaggggt ggtgccaggc acttgttgct aaagggctga ttaaagactt
     2821 aggtgaggcc ggcacgtcgt ggctgccgac cacctcttcc cgttccctct actgtgccac
     2881 cagccgcgat atgatcaagt tctccctgag cgtacggctg accaactcca tccgtactct
     2941 gtccgtgaaa gaagtgaagc gtgggatgcg cctggcacgc ctggctcaaa ccgacggctg
     3001 gcagatgcta caggtccgct tcccgacttt ccgggtaatg caggaggatg gctgggccgg
     3061 gctgctcgat cttaacggca acatcatgca ggaaagtctg tttgccctgc gtgaaaatct
     3121 gctggtggat cagccgaaaa gccagactaa cgtactggtc tccctgactc aggccgcacc
     3181 tgatggcggt gattcgctgc tggtttcggc ggtaaaacgc ctgagcgatc gcctcggtat
     3241 cactgtgcaa caggccgccc atgcatgggt cgatgcatac tgtcagcaag tgctaaagcc
     3301 gctgtttacg gctgaagcgg attacggcct ggtgctgctg gcgcatcagc aaaatattct
     3361 tgtccagatg cttggggatc tgccggtcgg atttatttac cgtgactgtc agggcagcgc
     3421 ttttatgcct cacgcgacag actggctcga ttctattggc gaggcgcagg cggaaaatat
     3481 tttcacccat gagcagttgc tgcgctattt cccttattac ctgctggtta actccacttt
     3541 tgctgtgacc gctgcgctgg gggctgccgg gctggacagc gaatcgaatc tgatggctcg
     3601 tgtacgagca tcgctggctg aagtgcgtga tcaggtgact cataaaacct gtctcaacta
     3661 cgtgctggaa agtccgtact ggaacgtaaa aggtaacttt ttctgttatc tgaacgatca
     3721 taacgagaac accattgttg acccttcggt gatctatttc gatttcgcta acccgctgca
     3781 ggctcaggag gtctgaatgt ctgaggcaaa cattattcac agcagatatg gactgcgctg
     3841 tgaaaaactc gacaagcccc tgaatcttgg ctggggactg gacaatagcg cggtgttgca
     3901 ctgtcccggg gagctgccga cagggtggct gtgcgatgcg ctggatcaga tatttatcgc
     3961 cgcaccacaa ctgtcagcag tggctctccc ctgggccgaa tggcgtgagg agccacaggc
     4021 gctgacgctt tttgggcagg taaaaagcga cattatccat cgtactgcct tctggcagtt
     4081 accgttatgg ttgagttcac cggcaaaccg ggcctccggt gaaatggttt ttgatgcaga
     4141 gcgtgagatt tatttcccgc agcgcccacc ccgtccacag ggtgaggttt accgccgtta
     4201 cgatccacgt attcgcagga tgctgagttt ccgcattgcc gatcccgttt ctgatgcaga
     4261 acgtttcacc cgctggatga atgatccgcg cgttgagtat ttctgggagc aaagtggctc
     4321 actggaggta cagaccgcct atctggagcg ccagctcacc ggtaaacatg cattcccgct
     4381 gattgggtgc ttcgacgatc ggccattcag ctatttcgaa atctactggg cggcggaaga
     4441 ccgcattggt cgtcactatt cgtggcagcc ctttgaccgt ggcctgcatc tgctggttgg
     4501 tgaacagcaa tggcgcgggg cccactatgt gcaaagctgg ctgcgcgggc tgacacatta
     4561 cctgctgctg gatgagcccc gtacgcagcg cacagtactg gagccacgca ccgataacca
     4621 gcgcctgttc cgccatcttg agcctgcggg ataccggaca attaaagagt tcgacttccc
     4681 acataagcgc tcgcgcatgg tgatggcgga tcgtcatcac ttcttcacgg aggtcggtct
     4741 gtaatgaatc acaaggactg ggattttgtt aaccgtcggc tggtggcaaa aatgttgtct
     4801 gagatggagt atgagcaggt tttccacgcg gaatctcagg gcgatgacca ctactgcatt
     4861 aacctgccgg gagcacaatg gcgcttcatc gctgaacgtg gtatctgggg ctggctgtgg
     4921 attgatgccc aaacgttgcg ctgcacggac gaaccagtac tggcgcagac gctgctgatg
     4981 cagctaaagc cggtgctgtc aatgagtgat gccactgttg cagagcatat gcaggatttg
     5041 tatgccacgc tactgggcga cctgcaactg ctgaaggccc gtcgcggact gagtgccagt
     5101 gacctgatta atcttgatgc cgatcgtctg caatgcctgc tgagcggtca tcctaaattc
     5161 gtttttaata aaggtcgccg tggctggggc aaagaggcgc tggaacgata tgcgccagag
     5221 tataccaaca ccttcagact gcactggctg gcggtaaaac gtgaacatat gatctggcgc
     5281 tgcgataacg atctggatat tcagcagttg ttgactgccg caatggatcc gcaggagttt
     5341 acacgcttca gtcaggtctg gcaggaaaac gggctggatc acaactggct gccgctgccg
     5401 gtacatccgt ggcagtggca gcaaaaaatc gccacagact tcatcgctga ttttgccgaa
     5461 ggcaggatgg tgtctctcgg cgagtttggc gaccagtggc tcgcccagca gtcgctgcgt
     5521 accctgacca acgccagccg ccggggaggg ctggatatca agctgccgct gaccatctac
     5581 aatacctcat gctaccgggg gatccctggc agatatatcg ctgccggacc actggcttca
     5641 cgctggctgc aacaggtttt tgcgaccgac gccaccctgg tgcaaagcgg cgcagtgatc
     5701 cttggtgaac cggctgcagg ctatgtgtcc catgaaggct atgccgcgct tgcccgggct
     5761 ccctatcgct accaggaaat gcttggtgtt atctggcggg agaatccgtg ccgctggctg
     5821 aaaccggatg aaagtcctgt tctgatggca acactgatgg agtgcgacga aaacaatcag
     5881 ccgctggcag gcgcatatat cgaccgctcc ggactggacg ctgaaacctg gcttacgcaa
     5941 ttgttccggg tggttgtggt tccgctgtat cacctgcttt gccgctacgg tgtcgcgctt
     6001 attgcacacg gacaaaatat aacgctcgcc atgaaagagg gggtaccaca gcgtgttctg
     6061 ctgaaagact tccagggcga tatgcggctg gtgaaagaag cgtttcccga aatggactct
     6121 ttgcctcagg aggtgcgtga tgtcacatcc cgtctgagtg cggactactt aatccatgat
     6181 ttgcagacgg gtcacttcgt gacagtactg cgttttattt cgccactgat ggttcgtctt
     6241 ggcgtacctg aaaggcgatt ttatcaactg ctggcagaag tgttgagtga ttacatgaac
     6301 aaacatccac aaatggcaga gcgttttgcg cttttctcac tcttcaggcc acaaatcatt
     6361 cgcgtggtgc tgaacccggt aaaactgacc tggccggacc tggatggcgg cagtcgcatg
     6421 ctgccgaatt accttgagaa tctgcaaaat ccgctgtggc tggtaactca ggaatatgaa
     6481 tcatgaaaaa aagtgtcgat tttattggtg tcgggacagg gccatttaat ctcagcattg
     6541 ctgcattgtc acatcagatc gaagaactga actgtctctt ctttgacgaa catcctcatt
     6601 tttcctggca tccgggtatg ctggtaccgg attgtcatat gcagaccgtc tttctgaaag
     6661 atctggtcag tgcagttgca cctacaaatc cctacagctt tgttaactat ctggtgaagc
     6721 acaaaaagtt ctatcgcttc cttacaagca ggctacgtac agtatcccgt gaagagtttt
     6781 ctgactatct ccgctgggct gctgaagata tgaataacct gtatttcagt cataccgttg
     6841 aaaatattga tttcgacaaa aaaagtcgat tgtttctggt tcagaccagt cggggagaat
     6901 attttgccca caatatctgc cttggtacag gaaaacaacc ttatttacca ccctgtgtga
     6961 agcatgtgac acaatcctgt ttccatgcca gtgaaatgaa tcttcgtcgg ccggacctga
     7021 gtggaaaacg gataaccgtg gttggtggag gacagagtgg tgcagacctg ttccttaatg
     7081 cattacgcgg ggaatgggga gaagcggcgg aaataaactg ggtctcacgg cgtaataatt
     7141 ttaacgcact ggatgaggct gcttttgctg atgagtattt tacacctgaa tatatttcag
     7201 gcttctccgg actgaaggaa gatattcgcc atcagttact ggatgagcag aaaatgacat
     7261 cggatggcat cactgccgat tctttactga ccatttatcg tgagttgtac caccgttttg
     7321 aagttctgag aaaaccaaga aatatccgtc tgctacccag tcgctcggta acaactctgg
     7381 aaagtagtgg tccgggctgg aggttattga tggaacatca tctggatcag ggcagagaga
     7441 gcctggaaag tgatgtggtg attttcgcca caggttaccg ttctgcattg ccacaaatac
     7501 ttccctcact gatgcccttg atcaccatgc acgataagaa cacctttaaa gtgcgtgatg
     7561 acttcactct ggaatggagt ggcccgaaag aaaataacat ctttgcggtc aacgccagca
     7621 tgcaaactca tggcatcgcc gaaccccagc tcagcctgat ggcctggcga tctgcacgta
     7681 ttcttaatcg cgtactgggg cgtgatttat tcgatctcag tatgccgccc gcactgattc
     7741 agtggcgcag cggcagccct gcttctttaa ctcactatac agcaaatatt caggaataac
     7801 gatgatgcgc aaaaagtata tgccccgggc tcttggtccg ctgcttcttg tcgtgctgtc
     7861 accagctgtc gcccagcaaa acgatgataa tgagatcata gtgtctgcca gccgcagcaa
     7921 tcgaactgta gcggagatgg cgcaaaccac ctgggttatc gaaaatgccg aactggagca
     7981 gcagattcag ggcggtaaag agctgaaaga cgcactggct cagttaatcc ccggccttga
     8041 tgtcagcagc caaagtcgaa ccaactacgg tatgaacatg cgtggccgcc cgctggttgt
     8101 cctgattgac ggtgtgcgcc tcaactcttc acgttccgac agccgacaac tggactctgt
     8161 cgatcctttt aatatcgacc atattgaagt gatctccggc gcgacggccc tgtacggtgg
     8221 cgggagtacc ggagggttga tcaacatcgt gaccaaaaaa ggtcagccgg aaaccatgat
     8281 ggagtttgag gctggcacaa aaagtggctt taacagcagt aaagatcacg atgagcgcat
     8341 tgccggtgct gtctccggcg gaaatgacca tatctccgga cgtctttccg tggcatatca
     8401 gaaatttggc ggctggtttg acggtaacgg cgatgccacc ctgcttgata acacccagac
     8461 cggcctgcag cactctaatc ggctggacat catgggaacc ggtacgctga acatcgatga
     8521 atcccggcag cttcaactga taacgcagta ctataaaagt cagggagacg acaattacgg
     8581 gcttaatctc gggaaaggct tttccgccat cagcgggagc agcacaccat acgtcagtaa
     8641 ggggctgaat tctgaccgca ttcccggcac tgagcggcat ttgatcagcc tgcagtactc
     8701 tgacagtgat ttcctgggac aggaactggt cggtcaggtt tactaccgcg atgagtcgtt
     8761 gcggtactac ccgttcccga cggtaaatgc gaataaacag gcgacggctt tctcctcgtc
     8821 acagcaggat accgaccagt acggcatgaa actgactctg aacagccaac tgatggacgg
     8881 ctggcaaatc acctgggggc tggatgctga gcatgagcgc tttacctcca accagatgtt
     8941 cttcgatctg gctcaggcaa gtgcttccgg agggctgaac aaccataaga tttacaccac
     9001 cgggcgctat ccgtcatatg acatcaccaa tctggcggcc ttcctgcaat ccagctatga
     9061 cattaatgat atttttaccg ttagcggtgg cgtacgctat cagtatactg agaacagggt
     9121 agatgatttc atcgactaca cgcagcaaca gaagattgct gccgggaagg cgatatctgc
     9181 cgacgccatt cctggtggtt cggtagatta cgataacttt ctgttcaatg ctggtctgct
     9241 gatgcacatc accgaacgtc agcaggcatg gttcaatttt tcccaggggg tggcattgcc
     9301 ggatccgggg aaatattatg gtcgcggcat ctatggtgca gcagtgaacg gccatcttcc
     9361 cctgacaaag agcgtgaacg tcagcgacag taagctggaa ggcgtgaaag tcgattctta
     9421 tgaactgggc tggcgcttta tcggtgacaa cctgcggact caaatcgcgg catattactc
     9481 gctttccaat aagagcgtgg aaaggaataa agatctgacc atcagtgtga aggacgacag
     9541 gcgccgtatt tacggcgtgg aaggtgcggt ggactacctg atcccggata ctgactggag
     9601 taccggtgtg aacttcaatg tgctgaaaac cgagtcgaaa gtgaacggtc aatggcaaaa
     9661 atatgacgtg aaggaatcaa gtccatcgaa agcgacagct tacattaact gggcgccgga
     9721 accgtggagt ctgcgtgtac agagcaccac ttctttcgac gtaagcgatg cagagggtaa
     9781 cgatattaat ggttacacta ccgtcgattt tatcagtagt tggcagcttc cggtgggaac
     9841 actcagcttc agcgttgaga acctcttcga ccgtgactat accactgtct ggggacagcg
     9901 tgcacctctg tactacagcc cgggttacgg ccctgcttca ctgtacgact acaaaggccg
     9961 gggccgaacc tttggtctga actactcagt gctgttctga cggggatgct tttgcaacaa
    10021 gggtatgctg taaacaacat ggtccttggt ggccatgttg tttacagcca gtataatata
    10081 cagccttaac aagatattcc gtctccggaa tgggctgttc accactgaat aacattatgt
    10141 ctgttttatg gatctttgac tgaaatttat cgcatatcct catttcattg tccgtctgaa
    10201 aactaacctt acaagtaagc gtcgtctcag caccgtctgg cagatcctga aattcctgag
    10261 aggatgatct gcgcccaata tcctgagccg gaactttgct gcaatgaagg ccaacgaaaa
    10321 atgggggagt atgttataga gtacttgcta cagggtaaaa agaggtacct gtcgctggtt
    10381 ctccgttcag tttaaccggg agattttctc ctgcgcctgt cataaagtcc ggtaatggag
    10441 attgttaata cgatggtgca cgatgcatcg tcagtgcccg gcccggagga tgccccgtag
    10501 ctacatacgg atcagggttg gcaatatcga atggcaggct ataggcaaag ttaaagcccc
    10561 atggagtagc acaaaatatg ccgcacaaag gaaacggtct gaataacgca gtgatggaga
    10621 acttcttcgg cacactgcaa aatcgcatcg tggtcgatcg ctaattcgtc gtgaatcact
    10681 gaacgatgtt ccatgcgaac agatattcga tcatatcgaa atattaaaca ttacccctcc
    10741 ccacattggg caaaaaaaaa caaaaaacga tcaattaacg atcaattagt attgattcac
    10801 ataatctctc atcattaatg agaacattgt cacatattta caatatagaa ctgttacttt
    10861 tagcaagctg ttagtaacca accgttacta ataattttaa ttcttaatac tttgttcatt
    10921 caataaatga gttgagagaa tatatgaata aaatatactc ccttaaatat agtgctgcca
    10981 ctggcggact cattgctgtt tctgaattag cgaaaagagt ttctggtaaa acaaaccgaa
    11041 aacttgtagc aacaatgttg tctctggctg ttgcaggtac agtaaatgca gcaaatattg
    11101 atatatcaaa tgtatgggcg agagactatc ttgatcttgc acaaaataaa ggtattttcc
    11161 agcccggagc aacagacgta acaatcactt taaaaaacgg agataaattc tctttccata
    11221 atctctcaat tccggatttt tctggtgcag cagcgagtgg cgcagctacc gcaataggag
    11281 gttcttatag tgttactgtt gcacataaca aaaagaaccc tcaggccgca gaaactcagg
    11341 tttacgctca gtcttcttac aaggttgttg acagaagaaa ttccaatgat tttgagattc
    11401 agaggttaaa taaatttgtt gtggaaacag taggtgccac cccggaagag accaacccta
    11461 caacatattc tgatgcatta gaacgctacg gtatagtcac ttctgacggt tcaaaaaaaa
    11521 tcataggttt tcgtgctggc tctggaggaa catcatttat taatggtgaa tccaaaatct
    11581 caacaaattc agcatatagc catgacctgt taagtgctag tctatttgag gtcacccaat
    11641 gggactcata cggcatgatg atttataaaa atgataaaac atttcgtaat cttgaaatat
    11701 tcggagacag cggctctgga gcatacttat atgataacaa actagaaaaa tgggtattag
    11761 tcggaacaac ccatggtatt gccagcgtta atggtgacca actgacatgg ataacaaaat
    11821 acaatgataa actggttagt gagttaaaag atacctatag tcataaaata aatctgaatg
    11881 gcaataatgt aaccattaaa aacacagata taacattaca ccaaaacaat gcagatacca
    11941 ctggtactca agaaaaaata actaaagaca aagatattgt gttcacaaat gggggaaatg
    12001 ttctgtttaa ggataatttg gattttggta gcggtggtat tatctttgac gaaggccatg
    12061 aatataacat aaacggtcag ggatttacat ttaaaggagc aggaattgat atcggaaaag
    12121 aaagcattgt aaactggaat gcattgtatt ccagtgatga tgttttacac aaaataggcc
    12181 ccggtactct gaatgttcaa aaaaaacagg gggcaaatat aaagataggt gaaggaaatg
    12241 ttattcttaa tgaagaagga acatttaaca atatatacct tgcaagcgga aatggtaagg
    12301 taatactaaa taaagataat tcccttggca atgatcaata tgcggggata ttttttacta
    12361 aacgtggtgg cacgctagat ttaaatggac acaatcagac ttttactaga attgccgcca
    12421 ctgacgatgg aacaacaata actaactcag atacaacgaa agaagccgtt ctggcaatca
    12481 ataacgaaga ctcctacata tatcatggga acataaatgg caatataaaa ctaacgcaca
    12541 atattaattc tcaggataag aaaactaatg caaaattaat tctggatggt agtgtcaaca
    12601 caaaaaatga tgttgaagtc agtaatgcca gtcttaccat gcaaggccat gcaacagagc
    12661 atgcaatatt cagaagcaga acgagtcatt gctccctggt atttctttgt ggaacggact
    12721 gggtcaccgt tttgaaagaa acagagagtt catataataa aaagttcaat tctgattaca
    12781 aaagtaataa tcagcagacc tcatttgatc agcctgactg gaaaaccggg gtgtttaaat
    12841 ttgatacatt acacctgaac aatgctgact tttcaatatc acgcaatgcc aatgttgaag
    12901 gaaatatatc agcaaataaa tcagctatca caatcggcga taaaaatgct tacattgata
    12961 atcttgcagg gaaaaatatt actaataatg gttttgactt caaacaaact atcagtacta
    13021 atctatccat aggagaaact aaatttacag gtggcatcac tgcacataac agccaaatag
    13081 ccataggtga tcaagctgta gttacactta atggtgcaac ctttctgaat aatactccta
    13141 taagtataga taaaggagca aaagttatag cacaaaattc catgttcaca acaaaaggta
    13201 ttgatatctc cggtgaactg actatgatgg gaatccctga acagaatagt aaaactgtaa
    13261 cgccgggtct ccactacgct gctgatggat tcaggctgag tggtggaaat gcaaatttca
    13321 ttgccagaaa tatggcatct gtcaccggaa atatttatgc tgatgatgca gcaaccatta
    13381 ctctgggaca gcctgaaact gaaacaccga ctatatcgtc tgcttatcag gcatgggcag
    13441 agactctttt gtatggcttt gataccgcct atcgaggcgc aataacagcc cccaaagcta
    13501 cagttagcat gaataatgcg atctggcatc taaatagcca gtcatcaatt aatcgtctag
    13561 aaacaaaaga cagtatggtg cgttttactg gtgacaatgg gaagtttaca acccttacag
    13621 tggacaacct tactatagat gacagtgcat ttgtgctgcg tgcaaatctg gcccaagcag
    13681 atcagcttgt tgtcaataaa tcgttgtctg gtaaaaacaa cctcctgtta gtcgacttca
    13741 ttgagaaaaa tggaaacagc aacggactga atatcgatct ggtcagcgca ccaaaaggaa
    13801 ctgcagtaga tgtctttaaa gctacgactc ggagtattgg cttcagtgat gtaacaccgg
    13861 ttatcgagca aaagaacgat acagacaaag caacatggac tctgatcggc tataaatctg
    13921 tggccaacgc cgatgcggct aaaaaggcaa cattactgat gtcaggcggc tataaagcct
    13981 tccttgctga ggtcaacaac cttaacaaac gtatgggtga tctgcgtgac attaacggtg
    14041 agtccggtgc atgggcccga atcatgagcg gaaccgggtc tgccggcggt ggattcagtg
    14101 acaactacac ccacgttcag gtcggtgcgg ataacaaaca tgaactcgat ggccttgacc
    14161 tcttcaccgg ggtgaccatg acctataccg acagccatgc aggcagtgat gccttcagtg
    14221 gtgaaacgaa gtctgtgggt gccggtctct atgcctctgc catgtttgag tccggagcat
    14281 atatcgacct catcggtaag tacgttcacc atgacaacga gtataccgca actttcgccg
    14341 gccttggcac cagagactac agctcccact cctggtatgc cggtgcggaa gtcggttacc
    14401 gttaccatgt aactgactct gcatggattg agccgcaggc ggaacttgtt tacggtgctg
    14461 tatccgggaa acagttctcc tggaaggacc agggaatgaa cctcaccatg aaggataagg
    14521 actttaatcc gctgattggg cgtaccggtg ttgatgtggg taaatccttc tccggtaagg
    14581 actggaaagt cacagcccgc gccggccttg gctaccagtt tgacctgttt gccaacggtg
    14641 aaaccgtact gcgtgatgcg tccggtgaga aacgtatcaa aggtgaaaaa gacggtcgta
    14701 tgctcatgaa tgttggtctc aatgccgaaa ttcgcgataa tcttcgcttc ggtcttgagt
    14761 ttgagaaatc ggcatttggt aaatacaacg tggataacgc gatcaacgcc aacttccgtt
    14821 actctttctg ataacagccc gggccgcgtt tgcggccctt cttctaccgg agagaatatg
    14881 tattaccctg tgacagacta tatcgctctt gctctcatta ttag
//
LOCUS       DAJTYG010000090        14664 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTYG010000090 DAJTYG010000000
VERSION     DAJTYG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.090"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            116..1417
                     /gene="rstB"
                     /locus_tag="OO568_003043"
     CDS             116..1417
                     /gene="rstB"
                     /locus_tag="OO568_003043"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6642318.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            1493..2422
                     /gene="tus"
                     /locus_tag="OO568_003044"
     CDS             1493..2422
                     /gene="tus"
                     /locus_tag="OO568_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6642319.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHEPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="OO568_003045"
     CDS             complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="OO568_003045"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6642320.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSIKAGR"
     gene            complement(3965..5611)
                     /gene="fumB"
                     /locus_tag="OO568_003046"
     CDS             complement(3965..5611)
                     /gene="fumB"
                     /locus_tag="OO568_003046"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6642321.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5810..6985
                     /gene="manA"
                     /locus_tag="OO568_003047"
     CDS             5810..6985
                     /gene="manA"
                     /locus_tag="OO568_003047"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6642322.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELGFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            7086..8594
                     /locus_tag="OO568_003048"
     CDS             7086..8594
                     /locus_tag="OO568_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6642323.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTQGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="OO568_003049"
     CDS             complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="OO568_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6642324.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYVAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(10124..10291)
                     /locus_tag="OO568_003050"
     CDS             complement(10124..10291)
                     /locus_tag="OO568_003050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR6642325.1"
                     /translation="MGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKK
                     VQQQLISDITN"
     gene            complement(10307..11497)
                     /gene="uidB"
                     /locus_tag="OO568_003051"
     CDS             complement(10307..11497)
                     /gene="uidB"
                     /locus_tag="OO568_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR6642326.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQV"
     gene            complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="OO568_003052"
     CDS             complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="OO568_003052"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6642327.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHGFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13693..14283)
                     /gene="uidR"
                     /locus_tag="OO568_003053"
     CDS             complement(13693..14283)
                     /gene="uidR"
                     /locus_tag="OO568_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6642328.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(14512..>14664)
                     /locus_tag="OO568_003054"
     CDS             complement(14512..>14664)
                     /locus_tag="OO568_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6642329.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
BASE COUNT         3678 a         3816 c         3493 g         3677 t
ORIGIN      
        1 atacatccgt ctgttccgga cattatcttc caaacaaggt cttatccagt tggcggttta
       61 cattgctcca cagtcgtgaa ttaatacgaa tagtattctg ttgagggcga tgccaatgaa
      121 aaaattgttt atccagtttt acctgttatt gtttgtctgc ttccttgtga tgtctctgct
      181 ggttgggctg gtgtacaaat ttaccgccga acgcgcgggc aaacagtcgc tggatgattt
      241 gatgaacagt tcgctgtatc tgatgcgcag cgaattgcga gagatccccc cacacgactg
      301 gggtaagacg ctgaaagaga tggatttaaa tctctctttc gatctgcgtg tcgagccact
      361 gagtaaatac catcttgatg atatttccat gcaccgactg cgtggcggcg aaattgtcgc
      421 cctggacgat cagtacacgt ttttgcagcg tatcccgcgc agccactacg tgctggcagt
      481 tggtcctgtt ccttatcttt attacctcca tcaaatgcga ttgctggata tcgccctgat
      541 cgcttttatt gctatttccc tcgcctttcc ggtgtttatc tggatgcgtc cgcactggca
      601 ggatatgtta aaactggaag cagcggcgca acgatttggc gatgggcatc tcaatgaacg
      661 tatccacttt gatgagggtt cgagctttga acgacttggt atcgcattta accagatggc
      721 ggacaatatc aacgccttaa ttgccagcaa aaaacagctt attgacggta tcgctcacga
      781 actgcgaaca ccgttagtgc gcctgcgtta tcgactggag atgagcgata acctgagcgc
      841 cgccgaatcc caggcgttga atcgtgatat cagtcaactt gaagctttaa ttgaagagct
      901 gctgacttat gcccgactcg atcgcccaca aaacgagctt catcttagcg aaccagacct
      961 gccgttgtgg ctgtcaacgc atctggcaga tattcaggca gtaacgcccg ataaaacggt
     1021 acggataaaa acgctcatgc aaggccatta tgcggcgttg gatatgcgct taatggagcg
     1081 cgtgctggat aatttgctca ataacgccct gcgctactgc cattcaacgg ttgaaaccag
     1141 cctgctactg tcggggaata gagcgacatt aattgttgag gatgatggcc cagggattgc
     1201 cccagaaaac cgcgaacata tctttgaacc ttttgttcgc ctcgatccca gccgggatcg
     1261 ctcaaccggc ggctgcgggc tggggctggc aattgtccac tctatagcac tggctatggg
     1321 cggtacggtt aattgtgaca tcagcgaact gggtggtgct cgcttctcgt ttagctggcc
     1381 gttatggcat aacatcccgc aatttacctc tgcctgacac tacgcgcacg atggtcaagt
     1441 cacaacgact gtgctataaa ataagtatgt tgtaactaaa gtggttaata ttatggcgcg
     1501 ttacgatctc gtagaccgac tcaacactac ctttcgccag atggaacaag agctggctac
     1561 atttgccgct catcttgagc aacacaagct attggttgcc cgcgtgttct ctttgccgga
     1621 ggtaaaaaaa gaggatgagc ataatccgct taatcgtatt gaggtaaaac aacatctcgg
     1681 caacgacgcg cagtcgctgg cgttgcgtca tttccgccat ttatttattc aacaacagtc
     1741 cgaaaatcgc agcagcaagg ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga
     1801 taacctttcg caagcagcgt tggtcagtca tattcagcac atcaataaac tcaagaccac
     1861 gttcgagcat atcgtcacgg ttgaatcaga actccccacc gcggcacgtt ttgaatgggt
     1921 gcatcgtcat ttgccggggc tgatcaccct taatgcttac cgcacgctca ccgttctgca
     1981 cgaacccgcc actttacgct ttggttgggc taataaacat atcattaaga atttgcatcg
     2041 tgatgaagtc ctggcacagc tggaaaaaag cctgaaatca ccacgcagtg tcgcaccgtg
     2101 gacgcgcgag gagtggcaaa gaaaactgga gcgagagtat caggatatcg ctgccctgcc
     2161 acagaacgcg aagttaaaaa tcaaacgtcc agtgaaggtg cagccgattg cccgcgtctg
     2221 gtacaaagga gatcaaaaac aagtccaaca cgcctgccct acaccactga ttgcactgat
     2281 taatcgggat aatggcgcgg gcgtgccgga cgttggtgag ttgttaaatt acgatgccga
     2341 caatgtgcag caccgttata aacctcaagc gcagccgctt cgtttgatca ttccacggct
     2401 gcacctgtat gttgcagatt aacgcccggc ttttatactg ccgaccatct gttctggccg
     2461 tacccagctg tcaaactcgg cttcgctaag ataccccagc gcaagggccg cagcttttaa
     2521 ggtcagccct tctttatgcg cttttttggc gatctccgcg gctttgtcat aaccaatgtg
     2581 ggtgttaagc gcagtcacca gcatcagcga ttcattgagt aattgattga ttcgctcacg
     2641 attcggttca atacccactg cgcagtgttt gttaaaactt tccatgccat ctgccagcaa
     2701 gcgcaccgat tgcaggaaat tatggatcac catcggacgg aagacgttca gttcaaagtt
     2761 accggaagcg ccacccatgt tgatcgccac gtcgttcccc attacctgac agcagagcat
     2821 ggttaatgct tcacactggg ttgggttcac tttccccggc atgattgagc tgcccggctc
     2881 attttccggg attgagattt caccaattcc gcagcgcggg ccagaggcca gccagcggac
     2941 atcattggcg attttcatca gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac
     3001 cagggcatca caggtcgcaa gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca
     3061 ggtaatgact gccagttcat ctgctacgcg ccgcgcatac tccggatggg tatttagtcc
     3121 agtacccacc gctgtaccgc caagagccag ttccgctacg tgaggcaggc tgtattcgat
     3181 atgtttgaga ttatgctcga gcatcgctac ccagccggaa atctcctgcc ctagtgttag
     3241 cggcgtggcg tcctgcaagt gggttcgacc gattttgacg atatcggcaa atgcacgcga
     3301 tttttcactc agcgtctgtg tcagggtttt aagctgcgga atgagttgct tgcgcagtgc
     3361 cagcagcgcc gcaacgtgca tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt
     3421 cacgtcgtcg ttagggtgaa ctttacgttc catcccgcgc acaccgccga gtaattcact
     3481 ggcccggtta gccagcactt cgttcatgtt catgttactt tgcgtgccgg agccggtctg
     3541 ccagatagcc agcgggaatt cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg
     3601 ccgaatggcg ctcgctttct cttcagacaa caagtctaaa tcttcattaa cttttgccgc
     3661 tgcgcgcttg gttagcgcca gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat
     3721 gcggaaatgc tccagcgagc gttgagtttg tgcgccccac agcttatctg ccgggacatc
     3781 aatcgccccc atcgaatctt tttcgctgcg tactgtattc atggcctgct cctcacctga
     3841 ttaatttttt ctttctgttt tgctttcgtt aagcaacttt tagctcactc attatttact
     3901 atttgataac aaatgtttgg tctttcgtgc catgtaaaaa aaccgccccg aagggcggct
     3961 ctgtttattt cacacagcga gtgcactgtg tgagttgtat ctgctggaag aagtcatttc
     4021 ctttatcatc cacaaggata aacgccggga aatcttccac ttcaattttc cagatggctt
     4081 ccattcccag ttccggatat tcaacacatt ccaggctctt aatacttccc tgcgccaata
     4141 cagcggccgg accaccgata ctgccaaggt agaagccgcc gtgtttttta caggcatccg
     4201 tcacctgctg gctgcggttg cctttcgcca gcatgatcat acttccgccc tgcgcttgca
     4261 gttgatcgac ataagaatcc atccgtccgg cggtcgttgg gccaagagaa ccggaggcat
     4321 aaccttccgg cgttttggcc ggacccgcgt agtaaatcgg atgatcttta atgtattgcg
     4381 gcagcccttc accgttatcc atccgctctt tcagtttggc gtgagcaata tcacgaccaa
     4441 cgataatcgt gccgttaagc gataagcgtg tagaaacggg atactgcgac aactgtgcga
     4501 ggatctcttt catcggacgg ttaaggtcaa cgcgcaccgc ttcgccttct cccgctttac
     4561 gcagctcttc agggatatat ttacctggat tatgttccag tttttcgatc cagatcccct
     4621 gacggttgat cttcgctttg atattacggt cagcagagca ggaaacaccc ataccaaccg
     4681 gacaggatgc gccgtgacgt ggcaggcgaa tcacgcggat gtcgtgagcg aagtatttac
     4741 caccaaactg cgcacccaga ccgagatttt gcgcttcgat cagtaattct ttttccagtt
     4801 ccacatcgcg gaacgcctga ccgtgctcat tcccttccgt tggcagttca tcatagtatt
     4861 tcgcggaagc cagtttcacc gttttaaggt tcgtttctgc agaagttcca ccaataacga
     4921 acgcaatatg atacggagga caggccgcgg tacccagcgt gcgcatcttc tcaaccaggt
     4981 aatttttcag tttccccggc gtcagtaacg ctttggtttc ctgatagaga tacgtcttgt
     5041 ttgccgaacc accacctttt gcgatacaga ggaatttgta ttcgtcgcca tcaacggcat
     5101 aaagatcgat ctgcgctggc agattggtgc cggtattcac ttctttatac atatccagcg
     5161 gcgcgttttg cgagtagcgc agattatctt cgatataagt gttatagaca ccgcgcgcca
     5221 gcgccgcttc atcaccacca ccggtccata cacgctgccc ttttttacca acaataatcg
     5281 cggtgccggt atcctgacag gttggcagaa cgcctttcgc cgcgatgtcg gagttacgca
     5341 ggaattgcag cgccacatat ttatcatttt cgctggcctc cgggtcacgc agaatgtcgg
     5401 ccacctgttg ttggtgagcc ggacgcagca taaacgacgc atcatgaaac gcctgacgcg
     5461 ccaacagagt taacgcttcg ggtgcgactt tcaaaatctc ctgcccttca aattcagata
     5521 cgctaacgtg ttcactggtt agcaggtaat actcagtatc atcttttttg agtggaaaag
     5581 gagactgata atgaaagggt ttgtttgaca ttgttctctc acttactgcc tggtttggtt
     5641 atgctctggg cgggtgttcc gttgccctgt taaaagcgag taacaatatc ctacacagtt
     5701 ttttaacaaa aactgagact agtacgactt tttgcggctc caggttactt cccgtaggat
     5761 tcttgcttta atagcgggat taatttccac attaaaacag ggattgatca tgcaaaaact
     5821 cattaactca gtgcaaaact atgcctgggg tagcaaaacg gcgttgactg aactttatgg
     5881 tatggaaaat ccgtccagcc agccgatggc cgagctgtgg atgggcgcac atccgaaaag
     5941 cagttcacga gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattgagag
     6001 tgataaatcg actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct
     6061 gttcaaagta ttatgcgcag cacagccact ctccattcag gttcatccaa acaaacacaa
     6121 ttctgaaatc ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg ccgccgagcg
     6181 taactataaa gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc
     6241 gatgaacgcg tttcgtgaat tttccgagat tgtctcccta ctccagccgg tcgccggtgc
     6301 acatccggcg attgctcact ttttacaaca gccacatgcc gaacgtttaa gcgaactgtt
     6361 cgccaacctg ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga ttttaaaatc
     6421 ggcgctcgac agtcagcagg gtgaaccgtg gcaaacgatt cgtttaattt ctgaatttta
     6481 cccggaagac agcggcctgt tctccccgct gttactgaat gtggtgaaat tgaatcctgg
     6541 cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga
     6601 agtgatggcg aactccgata acgtgctgcg tgcaggtctg acgcctaaat acattgatat
     6661 tccggaactg gttgccaatg tgaaattcga agccaaaccg gctaaccagt tgttgaccca
     6721 gccggtgaaa caaggtgcag aactgggctt cccgattcct gtggatgatt ttgccttctc
     6781 gctgcatgac cttagtgata aagaaaccac catcagccag cagagtgccg ccattttgtt
     6841 ctgcgtcgaa ggcgatgcaa cgttgtggaa aggttctcag cagttacagc ttaaaccggg
     6901 tgaatcagcg tttattgccg ccaacgaatc accggtgact gtcaaaggcc acggccgttt
     6961 agcgcgtgtt tacaacaagc tgtaagagct tactgaaaaa attaacatct cttgctaagc
     7021 ttattaaagg cttataacac cttcaggcgg ccagtccgcc tgatttcatt ttatggataa
     7081 tcattatgaa taaatcgctg gtagcggtag gcgtcattgt tgcgctaggc gtagtctgga
     7141 caggcggcgc atggtataca ggcaagaaga ttgaaaccca tctcgaagac atggtcgcgc
     7201 aggcgaacgc gcaactcaaa ctgacagctc ctgaatccaa cctggaagtg agttatcaaa
     7261 actatcatcg cggcgtattc agcagccagt tgcaactgtt ggtgaaaccc attgccggga
     7321 aagaaaatcc gtggattaaa agcggtcaga gcgtcatctt caacgaatcg gttgatcatg
     7381 gtcccttccc gcttgcccag cttaaaaaac tgaacctgat cccgtcgatg gcatcaattc
     7441 aaaccacgct ggttaataac gaagtaagca aaccactgtt tgatatggca aaaggtgaaa
     7501 cgccttttga gattaactcg cgcattggtt acagcggtga ttccagttcc gatatttcgc
     7561 tcaatccact gaattacgag caaaaggatg aaaaagtcgc ctttagcggc ggcgagttcc
     7621 agttaaatgc tgacagagac ggcaaagcca tctccctttc cggggaggcg caaagtggtc
     7681 ggatagacgc agttaacgaa tacaaccaga aagtgcagtt gacctttaat aatctgaaaa
     7741 ccgacggttc cagcacgcag ggaagttttg gtgagcgcgt aggaaaccaa aaactgtcac
     7801 tggaaaaaat gaccatttca gtggaaggca aagaactggc actgctggaa ggcatggaga
     7861 tcagcggtaa atcggatctg gtcaatgacg gtaaaacgat caatagccaa ctggattact
     7921 cgctaaacag cctgaaggta cagaatcagg atctgggcag cggcaagctg actttaaaag
     7981 tcggccagat tgatggtgaa gcctggcatc agtttagcca gcaatataac gcgcaaactc
     8041 aggcgctgct ggcacagcca gaaattgcca acaatcccga actttatcag gagaaagtga
     8101 cggaagcctt ctttagcgcc ctgccgctga tgttgaaagg cgatccggtg attactatcg
     8161 cgccgctaag ctggaaaaac agtcagggtg aaagtgcgct gaatctgtcg ctgttcctga
     8221 aagatccggc aacgactaaa gaagcgccgc aaacgctggc gcaggaagta gatcgttcgg
     8281 ttaaatctct ggatgcgaaa ctgaccattc cggtggatat ggcaactgag ttgatgactc
     8341 aggtagcgaa gctggaaggt tatcaggaag atcaagcgaa aaaactggcg aaacagcaag
     8401 ttgaaggtgc atcagcaatg gggcagatgt tccgtctgac caccttgcag gacaatacca
     8461 tcaccaccag cctgcaatat gctaacggtc agataacgtt aaatgggcag aaaatgtcgt
     8521 tagaagattt tgttggcatg tttgcgatgc cagctcttaa cgttccggct gtaccggcaa
     8581 ttccgcagca gtaattcacg gccaaaacgc cggagaattt tcactccggc gttttcatct
     8641 gaaaacaaac cagagattgc ctgatgcgct acgcttatca ggcctacctt ttcctgcaac
     8701 actttgaatt tatgagtttt tgtaggctgg ataaggcgtt tacgccgcat ccggcataaa
     8761 aaacgcgcac tttgtcaaca atctgaaacg ccggaggttt ttctctccgg cgttatttct
     8821 tacttcagca taaagtcata ctgaatactc attttcacat cccagtaatt caggcgatcg
     8881 cccagcgtac tgccatcgcg cttttcccgc cctttatcgg cagcaagaaa gaaattcatc
     8941 ccttgtaacg gcccttcgct gacccgatag ttcataccac ctaagatacc cgcgccttca
     9001 atccgcacat ctcgtttact ggattcatag ccgcgagtca caatcggtgc aaatgtcagc
     9061 gtaaattgcg gagttacacg gtagttgaca cctaattgcc aggactgcat tccttcacgg
     9121 ttacgatcaa gacttaatga aaagggatag ccaatgtcgg tatccaccac ggcaccattc
     9181 attgcatggc gcaaataacc accactgcca aatacgcttc cccacggcgc attccaggtc
     9241 aattgtccac taaccaacgc cgtttcattg ggctggctgg tattacgact taaaccttcg
     9301 agctgcgcgt aaaagccgag caactcccct ttcaacgtat aattcgggtc gagcggtatg
     9361 cttttctcga gaatactttg tatctgattg gaaacatctt tctgtaccga tgcccccaga
     9421 gaatagcgac tggtatcatc atgccaactg gcggctaaaa aagaacgtgc gcgatgttta
     9481 tcgtattttc ccgtctggct gttatacacc ccttcatcac gaataccggt tgttgaatca
     9541 gtccataaaa atactcgtga acgccacatt ccccacagat caatatttgg tgttaaagcc
     9601 caataaccta atgctccttc agaaactgtc ggtaatgtac gttgcgaact gccagccatt
     9661 aacggaatac gataaggtaa gctaccataa ctataatccg tcccgaagcg acctaattta
     9721 agcaggctat tttcactggg ttttattttt actgccccca gggcaaagcc aaaactttta
     9781 tcaccatcaa gataccaccg ggtactcata tcagcacgag cacctaattt ataaacataa
     9841 tacgctccgc cttcaacgcc aataatattg gaataataac cactattgaa atcgagcaat
     9901 cctccctgta cccatgcgta aatatctctt cccgcgccag cactgggctt atcagccctg
     9961 cgcaattcgt ttttcagtct gatgcgtagt cccgcttcat cgtcagccag ttgcgccacg
    10021 taagcagagg taaggccaga ggcagccgtc aggcaaataa cggccatggc cactattttt
    10081 ctcatagttg cacctttgat gttctgatta tttttattga atattaatta gtgatatcgc
    10141 tgattaattg ctgctgcact tttttacgat tatcaatttc aaccacgatt tctttgaatt
    10201 ttttatccgt gagcggataa aaccagataa taacgaatgc cagtagcata aatccgcaag
    10261 gtactaaggc aattgatgtg cggatgccca taataacttc cggcgtttac acctgattgg
    10321 cgatatatcc gcttaatccc aaaataaagg caggaattga acctccgatt gcctgaccgc
    10381 atttacgggt aaatgagaat agtgaatagg tgagcccttc aattcgcacg ccggtcaggt
    10441 attcaccgta ttctacggta tcagcttcca gtgcccacat cacagtcata gtaacgccct
    10501 gaccaattga agcgatggcc aacgcaacca acgccaccgg cagtgaccag acggaaaccc
    10561 agaagaatag cagataaccg caggttccca gcaaagcgcc aatcaggaag gtattctttt
    10621 taccgatcct cgcgaccatc cccggcacaa gcggtgccga tgccacagta ccaaccaggt
    10681 tttgcaccag taccagcaca gtgaacagcc cggtatcatt taacacatag cgcacgtaga
    10741 acaacgacga ggcgctgacc gcgaaggtcg aaatcagcac acacagcgca ccgatgcaca
    10801 acataaacag cgggcgattc cgtttcaggg tttgcaaact gatcttcaat gacggctgcg
    10861 ccacgatacg taccacattt tcacgcgtcg atttgaagca gatgaagtaa agcaccattc
    10921 cggcaatcgc cagcacaatc gtccagaaat ggtataccga caccatctct tccgggctgg
    10981 agttcttaat gctcggtcct atcagaaatg ccaggcatac aaaggtcaat gaagcggcaa
    11041 tcccacgagc cgcgcccaga cgggcgcgag attgtggttg ttgggtcatc gcggtagcaa
    11101 gtgaaccata aggaatattc accaggctgt agcaaagccc gaggcccatg taggtcaaat
    11161 atgcatacac cactttgcta ccatggctcc agtcggtcgg tacccagaat accagcacgc
    11221 tgaagatcat taacggcgca gtaccgaaga gtaaaaacgg gcggaatttt ccccagcggg
    11281 tattcacact gtccaccact cgtccggcaa agacgtcggc gaaggcatcg aatacccgca
    11341 ccagtaacag catggtgcct gccgcagcgg caccgacgcc agcgacgtcg gtgtagtaac
    11401 tcaataggaa gagcgccccc attgcgaagg cgaagttatt ggcgacgtca ccgaggctgt
    11461 agccgacgat ggtgcgccag gagagttgtt gattcattgt ttgcctccct gctgcggttt
    11521 ttcaccgaag ttcatgccag tccagcgttt ttgcagcaga aaagccgccg acttcggttt
    11581 gcggtcgcga gtgaagatcc ctttcttgtt accgccaacg cgcaatatgc cttgcgaggt
    11641 cgcaaaatcg gcgaaattcc atacctgttc accgacgacg gcgctgacgc gatcaaagac
    11701 gcggtgatac atatccagcc atgcacactg atactcttca ctccacatgt cggtgtacat
    11761 tgagtgcagg ccggctaacg tatccacgcc gtattcggtg atgataatcg gctgatgcag
    11821 tttctcctgc caggccagca gttctttttc cagtaccttc tctgccgttt ccaaatcgcc
    11881 gctttggaca taccaaccgt aataacggtt caggcacagc acatcaaaga gatcgctgat
    11941 ggtatcggtg tgagcgtcgc agaacattac attgacgcag gtgatcggac gcgtcgggtc
    12001 gagtttacgc gttgcttccg ccagtggcgc gaaatattcc cgtgcacctt gcggacgggt
    12061 atccggttcg ttggcaatac tccacatcac cacgcttggg tggtttttgt cacgcgctat
    12121 cagctcttta atcgcctgta agtgcgcctg ctgagtttcc ccgttgacag cctcttcgct
    12181 gtacagttct ttcggcttgt tgcccgcttc gaaaccaatg cctaaagaga ggttaaagcc
    12241 gacagctgca gtttcatcaa tcaccacgat gccatgttca tctgcccagt cgagcatctc
    12301 ttcagcgtaa gggtaatgcg aggtacggta ggagttggcc ccaatccagt ccattaatgc
    12361 gtgatcgtgc accatcagca cgttatcgaa tcctttgcct cgcaaatccg catcttcatg
    12421 acgaccaaag ccagtaaagt agaacggttt gtggttaatc aggaactgtt cgcccttcac
    12481 tgccactgac cggatgccga cgcgcagcgg gtagatatca cactctgtct ggcttttggc
    12541 tgtgacgcac agttcataga gataaccttc acccggttgc cagaggtgcg gattcaccac
    12601 ttgcaaagtc ccgctggtgc cttgtccagt tgcaaccacc tgttgatccg catcacgcag
    12661 ttcaacgctg atatcaccat tggccaccac ctgccagtca acagacgcgt ggttacagtc
    12721 ttgcgcgaca tgcgtcacca cggtgatatc gtccacccag gtgttcggcg tggtgtagag
    12781 cattacgctg cgatggatcc cggcatagtt aaagaaacca tggaagtaag actgcttttt
    12841 cttgccgttt tcgtcggtaa tcaccattcc cggcgggata gtctgccagt tcagttcgtt
    12901 gttcacacaa acggtgatac gtacactttt cccggcaata acatacggcg tgacatcggc
    12961 ttcaaatggc gtatagccgc cctgatgctc catcacttcc tgattattga cccacacttt
    13021 gccgtaatga gtgaccgcat cgaaacgcag cacgatacgc tggcctgccc aacctttcgg
    13081 tataaagact tcgcgctgat accagacgtt gcccacataa ttacgaatat ctgcatcggc
    13141 gaactgatcg ttaaaactgc ctggcactgc aattgcccgg ctttcttgta acgcgctttc
    13201 ccaccaacgc tgatcaattc cacagttttc gcgatccaga ctgaatgccc acaggccgtc
    13261 gagttttttg atttcacggg ttggggtttc tacaggacgt aacataaggg actcctcatt
    13321 aagataaaaa tactggtcaa cctttaatct gattagttaa ccaattctgt gttgcacaca
    13381 gttatctgtg agaaacccat ggaagagaaa tggaaataaa aagaactgac accgcgatca
    13441 aaaaaccaaa aactgcaata cccacagcgt gatatggatc gcattaatct ttaaaacagc
    13501 gttgacagaa gccataaaac aagcaatcga gcgttcgttc gagagtattg ggaacgataa
    13561 actctcatga catatggcta cggtgaatat tttggggggg aattctccga acacggggag
    13621 gataattttt gaacagagaa attacgatat gaaacgcggg cacagttatc accagcccgc
    13681 gaaatgagag gatcaggatg cggttaatat accgccaatc atcgcgcgta atccctgagc
    13741 aaacgacgct tcacgcgcca ggtcttccgc ttcgatatcg gccagtacac cgtaggtcag
    13801 atccagcagt aaacgtgaag tcattgccgt gttgatgtct gggcttatct cgcctttttg
    13861 ctgggcatca cgcatccgct gggcaacaaa ttcagtgatc gtcatatgct tatttttaag
    13921 catggcggcg acctgtgggt tacgcatccc ttccgccata atttcgacca ccagcgcccg
    13981 ttgcccaaag gcttcatggg tgagagagac aatagactcg accatatagt caacaaaatg
    14041 aatcccttca atcggttccc ggaaacgagc cagcgccctc tcctggtcct gtaagataat
    14101 cgcctgaatc aaggcttctt tggagatgaa atggtgatag agcgtcccag gactaatggc
    14161 gcaagattta cagatggctt tcatcgaggc actgtgaaat ccattttctg aaaaaatctc
    14221 tctggcagca ttgaggatcc gggtccgtgt cggttgtgct tcagtctgca tgttatccat
    14281 cattaaacca ttatagttag cgtgaataac tagtttaccg cgttgtcagg tgagcgccta
    14341 tcattgctgt tgaatagtgc gaaggtacac tctattcgta tacttttcac ccagcaattg
    14401 gttaaccaat tgccgtgatt tggctgccag ttgtttagca gagacgttga tgaattgcac
    14461 acgctatccc ggtaaacaac acatacgacc gtttaccggt tcgttagtgt attaattgag
    14521 ctcctgtacc ccaccaccgg agacggtgag aatttgtccg cttacccagc tggctgcagg
    14581 cgagcaaagg aacagcgccg cgttagcaat atcttgcggt tggcccagac gtctgatcgg
    14641 cgtgtgctgt aacatttttt gttc
//
LOCUS       DAJTYG010000091        13961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTYG010000091 DAJTYG010000000
VERSION     DAJTYG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.091"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..454
                     /locus_tag="OO568_003055"
                     /pseudo
     CDS             1..454
                     /locus_tag="OO568_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            514..621
                     /locus_tag="OO568_003056"
     CDS             514..621
                     /locus_tag="OO568_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6642330.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(602..1333)
                     /gene="yehW"
                     /locus_tag="OO568_003057"
     CDS             complement(602..1333)
                     /gene="yehW"
                     /locus_tag="OO568_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6642331.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="OO568_003058"
                     /pseudo
     CDS             complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="OO568_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
     gene            complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="OO568_003059"
                     /pseudo
     CDS             complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="OO568_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="OO568_003060"
     CDS             complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="OO568_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6642332.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDAPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="OO568_003061"
     CDS             complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="OO568_003061"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6642333.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MMVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7084..8799
                     /gene="dld"
                     /locus_tag="OO568_003062"
     CDS             7084..8799
                     /gene="dld"
                     /locus_tag="OO568_003062"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6642334.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="OO568_003063"
     CDS             complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="OO568_003063"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6642335.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9943..10530)
                     /locus_tag="OO568_003064"
     CDS             complement(9943..10530)
                     /locus_tag="OO568_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6642336.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10700..11278
                     /locus_tag="OO568_003065"
     CDS             10700..11278
                     /locus_tag="OO568_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6642337.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11395..12156)
                     /locus_tag="OO568_003066"
                     /pseudo
     CDS             complement(11395..12156)
                     /locus_tag="OO568_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12209..13645)
                     /gene="mdtQ"
                     /locus_tag="OO568_003067"
     CDS             complement(12209..13645)
                     /gene="mdtQ"
                     /locus_tag="OO568_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR6642338.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGMETDINASKTRQQLNDVVGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            13869..13952
                     /gene="yohP"
                     /locus_tag="OO568_003068"
     CDS             13869..13952
                     /gene="yohP"
                     /locus_tag="OO568_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6642339.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3321 a         3900 c         3522 g         3218 t
ORIGIN      
        1 ggcaatctac atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
       61 acactcacca cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact
      121 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
      181 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
      241 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
      301 ctcgctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct
      361 ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca gttggcaaga
      421 acaaggccat gatattttcc cactcggtat ttaatgattc gttaacaaat gcgctttact
      481 gtacaatcct ttcgttaaca taaggagtgc attatgcgca tagctaaaat tggggtcatc
      541 gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc aggttatacc
      601 tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg tctgcgatga
      661 tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta aatccgctaa
      721 gcccgatgat gatgggcgta cccagcgtac tggcccctac cgttgaggcg atcgtcgccg
      781 taccaatgtt gataatcacc gaagttcgca cgcccgccag aatcaccgga gccgccagcg
      841 gtagctcgac cttacgcagt cgctgacctc gactcattcc catacctttc gcaacttctg
      901 tcacgctggg atcaatcgct cccagcccgg caagtgtcac ctgcaggatg ggcagcacac
      961 cgtaaaggat caaggcgata atcgctggtt gcagaccaaa gccgatcacc ggaacggcaa
     1021 tcgccagcac tgcgacaggc ggaaaagtct gtccaacggc ggcaatagtt tccaccagtg
     1081 ggcgaaattc cgcgccccac gggcgagtaa cagcaattcc ggcacctgtg ccaatgatca
     1141 ccgcaaacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaga gctgcaaaac
     1201 tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc
     1261 tgtaaggcag ccagaaaatc agcgccacaa aaagagcaat gagccagaac agcggatcgc
     1321 gcaacatctt catacgctta cgcctccacc agcagatcct gaaaatgcag cgtgccgcaa
     1381 ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac aaacagggag
     1441 agcgcatcgc gtagcgtcat ctcttctgcc agtgcctcac cttctgcccg ttcttcgcga
     1501 cgcacgtaat ccgccacgct acgtaacgaa agcaggtgcc cccccagttc actacgtcca
     1561 aaaaactggc ggacaaaatc attcgccgga cgagtcagca tcgtcagcgg attcccctgc
     1621 tgcaccactt caccgtgatc catcaatacc agatgttctg ccagccgtag cgcctcatca
     1681 atatcatgag tgaccagcac aatagtgcgc cccagcaaac ggtgaatgcg cgtcatctct
     1741 tgttgcaacg cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc catcagtaag
     1801 acttgcggat cggcagccag tgcacgcgcc actcccacac gttgctactg accaccggaa
     1861 agctgatgcg gataacgctc acgcaaattt gactccagcc ccagtagcgc cattaattcg
     1921 tcgatacgat cgtcaatccg cgcccgcgac catttttgta attgcggcac ggtagcgatg
     1981 ttttgcgcca cgctccagtg ggggaacagg ccaatagatt gaatggcata gcccatccgg
     2041 cggcgcaact ccagtactgg cagcgagcga atttcttctc cggcaaagcg gatcactccg
     2101 ctgtcatgct ccaccaggcg gttaatcatt ttcagggtgg tggatttgcc ggagccagat
     2161 gtgccaatca gcaccgaaaa actcccttcc tgaaaattga gattgagatc gttaacggct
     2221 ttttgtgcgc cgaacagttt gctgacatgg ctaaattcaa tcattacgtt tcaccttcag
     2281 cagtgcgata agcaaatcga acagcgcgtc ggtaagcacc gccagaacaa ttaccgggat
     2341 cacccccagc aacactaaat caatggcgct gcttagcagc ccctggaaaa ccagcgcacc
     2401 aaaaccgcct gcgccgatta acgccgcaat caccgccata cctacagttt gcaccatcac
     2461 cacccgcagg ctgcgcagaa ataccggtaa cgccagcggt aactgaacat gcaggaaacg
     2521 ctgcgccccg ctcatcccca tcgctctggc gctctccagc acatcgcgcg ggatctggtt
     2581 caaaccgact accacgccgc gcaccagcgg cagcaaggca tagagcacca gcgcaatcag
     2641 tgcgggtgtc attccggttc ctgctatgcc gagcttcccc agccacggaa aggccgtaac
     2701 cagcgcggca agcggcgcaa tcaacaggcc aaagagcgcc accgaaggca cggtctgaat
     2761 gacattgagc agagaaaaaa ttgccccctg ccgcgcagtg gaaaagtagc accagatgcc
     2821 caacggcaca ccaatcacta acgcaggcag caccgcacca aacagcaacg tcagatgttg
     2881 tgccagcgcg tcgtcaaaca catcctgacg gttggcgtat tctttcattt agtgagagat
     2941 cgttaagcgt gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca
     3001 accagcgcca cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg
     3061 ccagcgccgc cgccagccag aaaccactgc cgaggctggt acgcgccagc gcactgccat
     3121 tttgcgccag ttgggtcgcc gcctttccag ctccccacac caacaatacg aagacgaatt
     3181 gcgccagaat gagtgcaaaa atgctgcctt ttttagcggg gacaaaacag gctgtcagcc
     3241 aggcgcaacc aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgaa
     3301 gcccttcacc cgagactaaa cgattaggcg cgtaactgat aaacggcagc gccgctgcga
     3361 ttgccgtcag caacaacagc agcgccagaa caggattaat acgtagataa gtcacgggaa
     3421 attacttcgc ccaccctttt tgtttcaggt agtcggcagc cacttttttg gcatccagtc
     3481 cttccactgc aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga
     3541 ctggctgtag ccactgtgcc atttgcggat actccttcaa caccgattca cgcaccactg
     3601 gtgcaggcgc gtagataggt tgcacgcctt gcggatcgct taaggtttgc agccccagcg
     3661 ccgcgaccgg agcgtcagtg ccgtaagcca ttgcggcatt aacgccagag gtttgctggg
     3721 cagcggcttt aatcgtcacc gccgtgtcgc caccagccag tgacagcaac tgatcctgac
     3781 cgagcttaaa gccataggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact
     3841 ctgccgaggc tgccagtttg aaggtgccgc cctcttgcag ataacgactc aggtcggcaa
     3901 gcgaagtgag tttgtttttc tctgccacat cctgacgcac ggcgatagtc caggtgttat
     3961 ttgcaggcgc gggcgtcagc cagattaact tgttgtgctc cgcatcgagc tttttgactt
     4021 tctcgtagcc ctgcccggca tttttccacg ctgcatcgtt ttcatcttta aagaagaaag
     4081 cgccattgcc ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca
     4141 ccacaggagt cgttccgagt tgcactttat tcaccgtcgg tacgccgtgg ctttcgagta
     4201 cctgcaagat gatgttgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa
     4261 cgggggaagc cgcctgtagc ggcaggctca cggctgccaa cataaccagt gaacctgccc
     4321 agagctttaa gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta
     4381 agagaaaagc atagttgata atgacagagt tagcctgagc ggaaggattc agttgcagaa
     4441 tcagataaat acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc
     4501 gccttatccg gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa
     4561 gggcgcaaac gtcgcgccct tactaaagca ttacagcaac tcaaactcgc ctttcttaac
     4621 gcgtgcggaa tcagtgccga taaatacatt gaacttgcca ggctcggcgt catatttcat
     4681 ctgttgattc cagaacttca gcgcctcaat atcgatcggg aagctgacag tctgagtttc
     4741 gcccggtttc agggtgattt tctcaaagcc tttcagctgt ttcacagggc gactcatgga
     4801 agccgtcaca tcctgcaagt acatctgcac taccgtggcc ccctcgcgct taccggtgtt
     4861 cgtcacctgc acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt
     4921 cacatcagag acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgcccc
     4981 gttagcttca tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg
     5041 accggtgttc agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat
     5101 tggcagcttg ccggacgggt tgtaatcgcc aaacaataca tcggcaattg cattaccgcc
     5161 ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag
     5221 cgccagcgga cgcccgttca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc
     5281 cgcaatcaag tcacgttggc cttgcggaat agtgatatcg gtccggctgg aggcttcgtg
     5341 cgccatcccc tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg
     5401 caccgcttca tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata
     5461 ctgattcagg aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag
     5521 cactttaccg ttttcaccga cggaattttt aatcccggtc agtacggtca cggattgatc
     5581 ggcaacaccg gctgcggacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc
     5641 aaccaccgca atggtggccg attttttcag cggtaacgtt tcgagacggt ttttcagcaa
     5701 cactaggctt tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt
     5761 ggtatccacc gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaaccc
     5821 catatcatat ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatggtcac
     5881 tttgccggat ttaatcaacc caggcagata cttcgagtag tactcgtcgc tcatactcat
     5941 gttgattccg gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg
     6001 tttaatcagc tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg
     6061 gtcgcgcaga acatctttca gcagccagga atcggaggtg gctggcgtgc cgttcagcga
     6121 gttcagcgcc accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat
     6181 ataatcatta aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc
     6241 taccgcgccg tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg
     6301 gcttttaccc tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc
     6361 ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg
     6421 tgcccaggtc atattcaggc catcatctgc cgcttcataa gcagagacac gcccgaccgt
     6481 tttcaccgca tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg
     6541 ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat
     6601 cacctgatcc tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac
     6661 ctgaccgtct ttgatcatct cgcggatcgc ctctttcgga ttatccgggc ctacgctgat
     6721 taagcgcagc tgaccaattt tctcatcaac catcattttc ttgagcagat cggtgacgaa
     6781 cgcatcccgc gcttcaggcg ttaatggatg gttaccaaat aaatcatccg ccagtgctgg
     6841 ctgcaggacc agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct
     6901 tcctttgttg cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat
     6961 gatggagaga gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct
     7021 gaattgtcat acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg
     7081 aaaatgtctt ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg
     7141 gtcggtcatt cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc
     7201 cgttctggtc agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg
     7261 cgggtgctga aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca
     7321 ggcctgaccg aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc
     7381 agcaccctgc gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat
     7441 ccgggcacca cgctctattc gctggaaaaa gccctcaaac cgctgggacg cgaaccgcat
     7501 tcggtgattg gatcatcgtg tataggcgca tcggtcatcg gcggtatttg taacaactcc
     7561 ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata
     7621 gatgaagatg gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg
     7681 gagcagatcc ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat
     7741 ggtcgtcacg cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg
     7801 cccgcacgtt ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag
     7861 ctggccgtct ttgcggtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt
     7921 tatatcggca ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac
     7981 ttcgaaaatc tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa
     8041 aaatacggca aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc
     8101 ttctttaatc tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg
     8161 cattttactg accgtgcaat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg
     8221 cgcatgaaaa actggcgcga taaatacgag catcatctgc tgttaaaaat ggcaggcgat
     8281 ggcgtcggtg aagccaaatc gtggctggtg gattatttca aacaggccga gggcgatttc
     8341 tttgtctgta cgccggagga aggtagcaaa gcgtttttac accgtttcgc cgctgcgggc
     8401 gcagcaattc gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcactggat
     8461 atcgctctgc ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc
     8521 cagctggtgc acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac
     8581 atagtgaaaa aaggcgtgga tgtgcatgcg ttaaaagaac agatgctgga actgctacag
     8641 cagcgcggcg cgcagtaccc tgccgagcat aacgtcggtc atttgtataa agcaccggag
     8701 acgttgcaga agttctatcg cgagaacgat ccgaccaata gcatgaatcc ggggatcggt
     8761 aaaaccagta aacggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat
     8821 cgccatccga attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg
     8881 ctttttgttt tttatagctc aacgctgctg ccggcacagg catcacttta ccggtttcaa
     8941 tccaggtacg caggcggctg gcatcggcaa aatgggtata tttgccaaac gcgtccatca
     9001 ctaccagcgc caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg
     9061 cattggtaaa gccggttttg gttaactgaa tattccagtt atcgcgatac accagatgat
     9121 tagtattgcg gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg
     9181 tagtacttaa ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt
     9241 cacgggcagt tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt
     9301 tcattccgag cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg
     9361 gataatggtg cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca
     9421 gcatatcttt acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct
     9481 ccggcgtctg gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca
     9541 caaccatcgc ggtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt
     9601 tcgaatagat cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt
     9661 ccggttgtga agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gcaaaaggca
     9721 cagccagcat cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc
     9781 agatagtggt tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc
     9841 gacgccatca taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag taaatgctgc
     9901 attgctgaca tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc
     9961 gtagccccat agtataacgg ttagggtgag cagcacttcc agtaccagca cgccaatcgc
    10021 cagcgtcgaa ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat
    10081 accaaggtaa agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa
    10141 ccagaccagt ggataaagcg ccaccagacc gcttaaaaac agcggagttg caacgtagcc
    10201 cgcgaagacc atgcagtgcg caagagacgg acgctgcggg taattgcgcg ccatccacca
    10261 gatgacccgc cccatcaccg caaccccagc cagcataacg ccgtaaaaca agacagccag
    10321 cgccagtccg gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat
    10381 ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg
    10441 ggtgtaatgg tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg
    10501 atgggaaaac agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct
    10561 gacagttcaa gtataagcca gcttgtgatt attttttgtt catccatgta atttgcaccg
    10621 tctgatgtat gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca
    10681 aagggagaca gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc
    10741 gctggttatc ggtagcctag cggaaggtga aaccgtgact ttgctgggag gcgttgcggc
    10801 acatcagggg ctattaaagt tcccactggt ggtactttct gtggcgcttg gtggcatgat
    10861 tggcgaccag gtgctctatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt
    10921 ctcgaaacat caggataaaa ttgagcgggc gcagaaactt atccaacgcc atccgtatct
    10981 gtttgtcatt ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg
    11041 tgccagccag ctgccgccga aaatctttct gccgctgaat attctcggcg catttgcctg
    11101 ggcgttgatt tttaccacta ttggttacgc tggtggtcag gtgattgcgc cgtggttgca
    11161 caatctcgac cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt
    11221 gggcgtgcgc tggtggctga aacggcgcgg gaagaaaaag ccggataatc aggcgtaaag
    11281 cagttgccac agataagcat tcactccaca tccggcaccg ggtgttccat gcctgatgcg
    11341 acgctggtgt cttatctggc ctaacccatt gaaatatcca cgcacctcac tgcgctattc
    11401 tgggttgaac tgcggattcg ccaacataaa gccgccatcc actatcaacg actgccctgt
    11461 cgtgtaattt gcgccctccg aacaaagcca caccaccagg ctggcaatct catgcgttgc
    11521 gccaaaacgc cgtagaggaa tcgaaggctc cgcgtcgggc ttcacgtcgc tatcatccat
    11581 gctgttcatt ggcgtggcta tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct
    11641 gaccaactcc agcgccatcg ttttggttaa cccgccgagc gcatgtttag cggctgtgta
    11701 ggcgctggca tccggcagcg gcgtatgttc atgtaccgac gtaatgttga tgatgcgacc
    11761 gccctgccct tatttcacca tctgacgagc cgcaatttgc gagcataaaa atgcaccatc
    11821 aacatcaacg gtaaaaatct tgcgccactc atcaaaagcc atatcaagaa acggcgcttt
    11881 ggtcattgca cccgcattat tcaccagcac atcaatacgc ccaaatcgct ggatgagtgt
    11941 ctccagcgcc tgcgcacctt ctggcagatt gccgagatcc agttgcacga tctccgcacg
    12001 tacgccgtgg gtaaccacct cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca
    12061 ggtaatgcca atatcaaacc cctgctgcgc cagtaataac gcgcactctt tgccgatccc
    12121 cgaatcggaa gcggtaatta tcgcgaccta tgccatcgag ttctccactt aacgctgaat
    12181 aaacgttaag tatagaaggc gcatatcatc agcgtttgta acccccgccc agcgcaccgg
    12241 tgagtcgaat ggaggcatcc agccactgcc cttgcaataa caggccattg gcccgctcac
    12301 gcagcgccgg gattctggct tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc
    12361 gggcctgcgc aagacctacc acacgcaagg catcgcgctc aatttgcgcc tgatgctggt
    12421 ttttctccgc cagtgtctga acctgactgg ctgcccgcgc cacgtcattc actgcttcaa
    12481 ccaccgcttt gttgtagctg gcgatagaca agttgctttc ggcttttgcg atatcgagat
    12541 tggcgttaag acgaccacta tcgaaaatag gtagcgtcag gcctgccgta acgcccattt
    12601 gctgcgcgga atgacggaac agatcgctta agtgcaacgc atcctgttgc aggaaggcca
    12661 tcaggttgat gtcaggataa aatgccgctt ttgccgcatc aatagtgctt aacgatgact
    12721 caacgtacca gtgcgccgcc tgcaaatctg cccgccgggc cagtaaggag taccccagtt
    12781 catcaggaag ctggcttgcc actttcggca acgcgaccgg tttaagcttc aatgacttag
    12841 tctggtgatt tgtgagtgca tttaaccgtg cctcaataat tttcattttc cccacgacat
    12901 cgttgagctg ctgccgggtt ttgctggcat taatatcggt ttccataccg tcaactgaag
    12961 aagtaatccc gttctgatat agctggcgat cggtcgcgat aatggtgttc tgctcttttt
    13021 ctatttgctg caagaccgtg tttaacgccg cctgggtttg ccactcccag tacaggcggg
    13081 ctacgctgcc agccagcaat tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg
    13141 tacccaggcg ggcagtaacc tccgcccgat tctttcccca gatatcgaga tgccagcccg
    13201 ccgttaagcc aaaagttccg ttggtgtacc acggaccggt cgtacctgcg gccggatcgt
    13261 tcagagcaaa cggccccatt agcccttctg ccgacatttt ttgccgctcc atatccgccg
    13321 aaaagtcgat ctgcggacca tcctgagtgg caactgcctt cgcctgggct tcagctagct
    13381 gaatgcgctg ttcagccacc tgcatatccg gtgcgttctg tagtgcattg ttaattaatg
    13441 aagtgagctg attatcgtga tactccagcc accattggct gtctggccaa ccatttttca
    13501 gcgccgtggg taatgcggtg tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc
    13561 gggtttcatg cataggcgca cacccggcca gcatcagtaa cagcggaaaa caggcgatgg
    13621 ctggataaaa ggaatcacga ttcatggggg aataatcagg taagaaacgg tgcgcggaga
    13681 ttaccgtgtg ttgcgatata ttttttagtt tcgcgtggca atacatcagt agcaataaaa
    13741 cgacataccc agaaaaatat agactaagta aatgatatct tccgatttat cttaatcgtt
    13801 tatggataac ggcaaagggc ttcgtttttt cctatactta ttcagcactc acaaataaag
    13861 gaacgccaat gaaaattata ctctgggctg tattgattat tttcctgatt gggctactgg
    13921 tggtgactgg cgtatttaag atgatatttt aaaattaatt a
//
LOCUS       DAJTYG010000092        13890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTYG010000092 DAJTYG010000000
VERSION     DAJTYG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.092"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..915)
                     /gene="wcaD"
                     /locus_tag="OO568_003069"
     CDS             complement(<1..915)
                     /gene="wcaD"
                     /locus_tag="OO568_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6642340.1"
                     /translation="MSTSIRICSYLLLLLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSL"
     gene            complement(890..2107)
                     /gene="wcaC"
                     /locus_tag="OO568_003070"
     CDS             complement(890..2107)
                     /gene="wcaC"
                     /locus_tag="OO568_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6642341.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKHQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2104..2592)
                     /gene="wcaB"
                     /locus_tag="OO568_003071"
     CDS             complement(2104..2592)
                     /gene="wcaB"
                     /locus_tag="OO568_003071"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR6642342.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2600..3433)
                     /gene="wcaA"
                     /locus_tag="OO568_003072"
                     /pseudo
     CDS             complement(2600..3433)
                     /gene="wcaA"
                     /locus_tag="OO568_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3526..5688)
                     /gene="wzc"
                     /locus_tag="OO568_003073"
     CDS             complement(3526..5688)
                     /gene="wzc"
                     /locus_tag="OO568_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR6642343.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPSVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQVQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5691..6134)
                     /gene="wzb"
                     /locus_tag="OO568_003074"
     CDS             complement(5691..6134)
                     /gene="wzb"
                     /locus_tag="OO568_003074"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6642344.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6140..7279)
                     /gene="wza"
                     /locus_tag="OO568_003075"
     CDS             complement(6140..7279)
                     /gene="wza"
                     /locus_tag="OO568_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6642345.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLIPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7588..7737
                     /locus_tag="OO568_003076"
     CDS             7588..7737
                     /locus_tag="OO568_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642346.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            7938..9521
                     /locus_tag="OO568_003077"
     CDS             7938..9521
                     /locus_tag="OO568_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6642347.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9973..11826)
                     /gene="asmA"
                     /locus_tag="OO568_003078"
     CDS             complement(9973..11826)
                     /gene="asmA"
                     /locus_tag="OO568_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6642348.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11848..12429)
                     /gene="dcd"
                     /locus_tag="OO568_003079"
     CDS             complement(11848..12429)
                     /gene="dcd"
                     /locus_tag="OO568_003079"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6642349.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12521..13162)
                     /gene="udk"
                     /locus_tag="OO568_003080"
     CDS             complement(12521..13162)
                     /gene="udk"
                     /locus_tag="OO568_003080"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6642350.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVKVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13480..>13890
                     /locus_tag="OO568_003081"
     CDS             13480..>13890
                     /locus_tag="OO568_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCR6642351.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPL"
BASE COUNT         3226 a         3667 c         3508 g         3489 t
ORIGIN      
        1 aagtgagcca aatctgacta caccgtcaat atgggtcaaa gaataaccga ccatcaccag
       61 cggaccgaca atacgataat acgatgacga tccttccgta cctaaatcgc ccagacgggt
      121 ggaaatataa ggaaacgcga ttaccacacc aaccaggaat acagccagag aaatcaatgc
      181 taacggtaat tttttcttaa tcgcctcttt attcagatat tgaaatgccc actccagcaa
      241 ataaaacagg ataaaggtca taacccctga aaacgatccg gataatatta tccctgcgag
      301 aatcatagca tctgttttag gcgttttgat accaaactgt ttgatgctga gccaaattga
      361 gattaatgcc agagcgaaaa atgccggttc gaaataaagt gctgtcgtgc gcttgccgcc
      421 gaatttaatg aaattcagca catagctgtt gctgtaaatc agatatttcg aaatcgactc
      481 cataatactg ctgccaccgg tgaggataat ttgcgccatc tccaccgccg ccagcgccac
      541 caccagtccg accaccagat aaaagaaacg taatatctta cgatggttgt gtggcgaaat
      601 tgttttaaaa cgaatactcc acaccatgcc aataatgatc acaatataga caaacagcat
      661 agttgaagtg acgtatttac cggcatccaa cgactgacca aacaggtagt taaacgccgt
      721 gagtcccgcg ccaatcccta aggcaatcat caatttttta atgctgatgc gttctaaaaa
      781 cagcagtaac aagacaggta aaaaagtgac gatggtgatg gggaaacttt cgccaagctg
      841 ggcgattttg acgttaacca gcaggtagat cagtagcagc agcaggtagc tacagattct
      901 gatagaagtt gacatactcc tccagcatct gttgtccact gtaggcggcg cgactgcgtt
      961 ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc gatttccggt ttgcttaact
     1021 gtaccagttg cagcacctct tcttcactga cggttttacc accggatttt tgcagcactt
     1081 cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc aatcgatagc gcctcacaca
     1141 aaatcagcgg gtagttatcg acacgagaac tgaacaccag cgcatccatc tgattgagcg
     1201 cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt aaccacgttg ccagcggtga
     1261 acggcgagaa cttaccaaag gtatgcagtt cgattttgtc gcccagcgcc atcatctcac
     1321 gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc atgcgccacc accgcgattt
     1381 tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc cagaatcgct tcggttgcca
     1441 tatcaatgcc attattgata atccggcaac gccctggccc gtacagacta ttgaaagcgt
     1501 cagccacatg ctggctgggg gaaataaact gacagcccag cgccagcatc tcgcggaata
     1561 actggtgttt gcccgccacc agctggtgcg cgcgatcaat cttcaccggc ggatagttat
     1621 ttaaggtcgg gcatttctgg cagcccgttt tccagccttc gcaaccgtcg gtaaaggcgc
     1681 agcgtccggt tacactccag tggtcgtgca gcgtccagac cagggtgaca tccggtttat
     1741 ggtttttcac tttttcgcaa aagcgcacca cgctgttaag attcaaccag tagctgtgca
     1801 gcacatgaaa atgcaggacc accggacccg gtgtgcgagt aatggtgcga tataactcat
     1861 tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa cgcaatgttc gccatcgcgg
     1921 tcatccgcgg cgtatgtttg atgacctgcg gatagttctg atggctgaca ctctctttgc
     1981 cgcctttgcc gtagccgtag acaaaatgtg acgccagccc ctgttgcagc gcacgctggt
     2041 ggagatctaa cgccacacct gctgccccgc cttccgccag tcgcacatta aattgcagaa
     2101 tattcattta attaccttca ctcgcgcttt ttctcccacc accagcgcgt tgtccgggac
     2161 agagtcgagc accacgctgc ccgcacccac ggtgacgttg ttaccaagcg tgatatcacc
     2221 aagaataatg acgttggcac cgagttcgac gccgttgcca atgtgtggac atgccatgtt
     2281 atcggcaccc cgattgccga tagtgacgcc gtggcgaatg gtgaaatcat cccccgccac
     2341 cacgttttta ttgatcacta cggcgtaacc gtgatggata gtaaagcggc ggccaatggt
     2401 cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg ataatgcgat acagcaccag
     2461 cagcggggcc gcccacagat tgttgaggac gttctttttg cgccacaccg aacaaaaatg
     2521 agcgacgcga taggcaagta ccatgcagca cgggcgtaaa ctccagctgt tggcgcgcag
     2581 atcttccagc atctttagcg ccccgaatac catcagccag acgtttgccg ttacgcaccg
     2641 acagcagcgt caacaaggtg cgccaggtca tacgtttatt gcggatctgg tagggggtaa
     2701 acagctgata ttttttactg gcgcggtcga atttgtcttt gtgcttgcgg taaaagtgga
     2761 agtatccgga gaattttttc ggtgacgagg tgatctgcat ttcgccgtga ttgatatgca
     2821 ggatctgcgt cgcctcttcc actttccacg gttcgccgta ctccaccacc atccgcagga
     2881 atatgtcgta atcctgtgct gctttcagtt cggtatcgaa caaacactct ttgaaacgcc
     2941 atgcccaggt aaagacctga ttgccaatga tattacgttt atagaacagg cggcgtgagt
     3001 acggcgattt gggatacagt ggtaagctcg ccggttggga ataaacttct ccctggcaaa
     3061 cgtagtcgtt agcgtacaaa aaggcgtgtg tgaccagttg ctgtttatgg gcgaggaaga
     3121 cgctcaggcg gttgggcgtc cattcatcat catcatcgat cccggtgatg tattcccctt
     3181 gcgccagcat aatcgcctgg ttacgcaccg cgcacgcccc gctgttaatg tcgttgtgga
     3241 tgtaagtgat acgcggatcg ttgagggcag tgacgtactg ttgcagttgt tcccaggagg
     3301 tggagcaatc atccacgatg atcatctccc agttgctgta gtcctggcgc aggaccgatt
     3361 ttatcgcccg aatcgccagt tgttggcggt tccaggttgg catatagatt gagatcagcg
     3421 gattgtcttt cataggttgt tctcccggct tatgagctgg tttgcttgcc ggatgcggcg
     3481 tgagcgcctt atccggccta cggggcggtg cgaatgcagg cgtcgttatt tcgcatccga
     3541 cttatattcg tattcgtaat agccataatc ctgatacgcg ctggcgcggc ggaagatgga
     3601 gttcagaatc acccctttca ccggaatacc gttttgctca aagcggctca gactggtttc
     3661 cacttctttc aatgtgttga ccgcataacg cgccaccatt aacgtggttc cgacatgacg
     3721 accaacaatt gccgcatcgg tcactgccag aatcggcggc gtatcaatca acaccaggtc
     3781 atagttttta ctcgcccagt tcaccagttc ggcaaagcgt tcgctcatta acagttcaga
     3841 aggatttggc ggcacctgac cgcgcgggat caggtcaaat ttggcaatag aggtcggttt
     3901 agcagcggta gtaatatcgc cctgaccaat cagaatctcc gacagcccat tgacattatt
     3961 ggtgcccaac agctcgtggg tgtagccttt gcgcatatcg cagtcgatca acaacacgcg
     4021 tttattggtc tggctgatca ccgccgccag gttggcgcag acaaaggttt taccgattga
     4081 cgggctaacc ccggtcatca tcaacacatt gttctgcacc tgcatcatcg cgaagtgcag
     4141 actggtacgc agactacgga tggcttcaat cgccagatcg gttggattcc ccactgccag
     4201 tagctggctc tgtttatagc gtttaacccc tttgatggtt ttgacgctat cgcgcgcttt
     4261 ctgccactcc gacagcggga tgctggcata gacgctgatt ccgtgttctt ccagcacttg
     4321 cgggctttcg atgccgcgat taaacaacga gcgcagcagc acacccacga tagagagcat
     4381 caggccaagg ataatcgcgc cgaggataat cagccctttc ttcggtttta gcacgctagg
     4441 ctgggtgatt gccgggtcaa taatgcgcac atcgccgacg gtgctggctt cggtgatttt
     4501 cagctcctgc tctttattca gcagttgcat atagacctgc tgaccagact cgacatcgcg
     4561 ggtcagacgg acaatttcct gctgggtttt cggcatcgcc gttacgcgac cgttaagttt
     4621 ggctttctct tcttccagcg cctgacgttt ctccagcagc gtgcggtacg ccgggtgaac
     4681 tttggtgtac agcttggaga tttccgcctc tttaaaggtc agttcgttca actgcgcgtc
     4741 gatgttcacc atcgaatcga gcaccgcttt cgcttcaagc ggcagatcaa cagaatcttt
     4801 atcctgacgg aaggcattca gtttgttttc ggcaacatcg aggcggctac gtacttccgg
     4861 taactgttgc gcgaggaaag cgaggctttt cgacgcttcc gccgatttgc gctcaatatt
     4921 ttgttcctga tagttacgag cgatgctgtt aagaatgtcg cggatctgtt cgcgatcttc
     4981 accggtataa gtcaggctca gtacgcctgc gtctttgccg ttctccgtta ccgtcaggct
     5041 gttttgcagt tggttgatca tccccaacgt ggagtatttg gtgacggtaa actcactgcc
     5101 cgggcgggcg tgaatggctt caaccatcag cgtgacgcct tcttttttca gcatctggcc
     5161 cgcttgccca cgggcgctaa agccgccatc actgctcagg gtgtagtttt tgtcatccag
     5221 cacattaagc gtaaacacct gatccgccat ctctttcggg cggttaaagg tagtcacttt
     5281 caccgtctcg ttctgacgcc ccatcaggcg atcccagccc gccccgaaaa tcgggaacgt
     5341 gtttttgctc accgcaatat cgaggtcgag atcgtccacc gttttaccaa gcaccagacg
     5401 cgaacgaatc aactggatct cggcgtccga tgcaggcggt ttgttggcta atgccgagcc
     5461 gatatcctgc accagcgaat tgccgctgtt ttgctcgatt tgtaccagtg cgtcggcact
     5521 ataaatcggc gtggcgaaga aagtgtaaac cacggcacag agggcaaata cgacggtgat
     5581 gccaatcacc caccagcgcg cttcaatgac ggtgccgacc aggcgaccaa tatcgatttc
     5641 atcactgccc gttaccggag cggcatgttg ttttactttt tctgtcattc ttatacctgc
     5701 tctgcgttca atgcctgcgc ccactggcgg gcagaccgtt caagtaatgt gtacaccgct
     5761 gcaaacgttt cccggctttt gcgatacgga tcggggattt cacattcgtt atcccagtga
     5821 ccaaacagca tcactttgcc gcgcatctcc ggtgccatct cgcataagcg ttcgatatgg
     5881 cgcttatcca tggtcaaaat caggtcatag ttgcgacaca ggcggcggct gatttgtcgt
     5941 gcacagtggc cttccagaga cagttgatgt tctgcggcga cgctgatagc ggtaggatca
     6001 gcgcccttac cgaccagcgc gccgagtcca gcggactcca ctttcagttc cgggtgataa
     6061 cgttgcagta agcgttccgc catcggggaa cggcaaatat tgcctacaca gacaactaag
     6121 atgttgttaa acatgacgat taccagttat gaatgtcgct ggctgtatcc gtcatgtaac
     6181 ggacaccgct aatagttggc agcaactgat tgatcagacg gttccagcgg gaaaccgggg
     6241 cggtggtgac atacaccacg tcataaggtt gcaggcggaa ttctgtcgcc atcaccagcg
     6301 acgtcgcatc ggacatatcc agctggtaga tattggcaat cttgccgtta cgcccgccct
     6361 cgcctttcag cggacgaatg acaaagatgc cgctggcgtt ggaggtggtc atgtcgatac
     6421 cttcggcatt gcccagagct tcagtcaggg tcatgccgct aaagtccatt ttcagggtgc
     6481 tctgtttctt cacttcaccc atcacaaata ctttcagatc atcattacgc ggcacgtaga
     6541 gaatatcgcc gggataaagc agtcggttct gattgaggtc gccgttttgc atcagcgcct
     6601 gtaaagaaat gcgttcttca cgaccattgt gtgttagcac cacgttgcgc cagtcagcgg
     6661 tgtcggtcag gccacctgcg gcgttgatgg cgtcgagaat ggtcagtggt acgttggtga
     6721 tcgcctgttg accggattta ttcacctgac ctgagatata ggccttttgt gagcggaagg
     6781 cggcgatatt aacgtccacc tgcgggtcag cgatgtatgt cgctaagcgc ccggtaatat
     6841 cactgcggat ttcagcgagc gttttcccga ctacgtgaac cttgccgata tacgggtaaa
     6901 acatagtgcc gtcaggctgt acccagttgc cggtgtcgct ggagctgcgg tactgaccag
     6961 ctggcgtggt gagttccggg tgatcccaga cggtgacatt aagaacgtcc cccggcccga
     7021 cgcgatactg gtaattcgcg atctcgcttt ccagcgtcat attggggcgc gctacattcg
     7081 ggcgtgggcg taattggtca atcaggcgcg ggatcagcgg ataaacattc accattttgt
     7141 cgagatcgaa atcagcgtcc tgctgtttga tgacgtcttt gcccatcgtc gacatattgc
     7201 tgcccggaag tactgtgcaa ccgcttatca aggttactga caccaataat ggcatcaatt
     7261 tcattttgga tttcatcatt gtttatttat cactttggca gagtaattat cctgtgcact
     7321 attaatagca atgtcgccat gcacatttac cttgcagtta attgaataaa aatttaactg
     7381 gcatcagtcc taaaaaaatt gatttcatcc gcaggctatt gacagaataa ttcagactgg
     7441 tctttcaggc atccagacac gctaccgccc ctggcttttt agctaccaat acactgattg
     7501 agcttaattt ttcaaaccct ctctgcatac aatcgttgat gagaaagggt tattgcggaa
     7561 attaacttcc gaatataagg tgacattatg gtaattgaat attggctcgc caataatgca
     7621 ggaggaagtg ttacagctaa cggaatagca ggcaaggtaa caattcggca attggctatt
     7681 tttaagaatt atattaagtg tcattcaata tggtttttag gagtttcttt aggttgacaa
     7741 tatttaatat tgcgtctcca catgcgatat ttcttaaata atgttttatt attaccgctt
     7801 ttaattcagg gataatgtga ggttattatc ctcaaataat atgagataaa tagtgctgca
     7861 acattgcatt tttgccccga tttatccatg atcgaattgt gacatttgtc atacaacgaa
     7921 taggttttgt acttactatg gaatggattg ccgatccgtc tatctgggcc gggttaatca
     7981 cgctgattgt gatcgaactg gtcctcggca ttgataacct ggtctttatt gccatcctcg
     8041 ccgaaaaact accgccgaag cagcgcgacc gcgcacgggt taccgggctg ctgctggcga
     8101 tgttaatgcg cctgttactg ctagcgtcaa tctcctggct ggtcaccctg actcaaccgc
     8161 tgttcagctt ccgctcgttt acctttagcg cccgcgactt aatcatgctg tttggtggtt
     8221 tcttcctgtt gttcaaagcc acgatggagc tgaacgaacg gctggaaggg aaagacagca
     8281 ataatcccac acaacgcaaa ggtgcgaagt tctggggcgt ggtgacgcaa attgtggtac
     8341 tggacgccat tttctcactc gactcagtga ttaccgccgt cgggatggtc gaccatttac
     8401 tggtcatgat ggccgccgtg gttatcgcta tcagcctgat gttgatggcc agcaagccgt
     8461 taacgcaatt tgttaacagt cacccgacca tcgttattct ctgcttaagc ttcctgttga
     8521 tgattggctt tagcctggtg gcagaaggtt tcggcttcgt cattccgaaa ggctacctgt
     8581 acgctgccat tggtttctcg gtgatgatcg aggcactcaa tcagttggct atctttaacc
     8641 ggcgacgttt tctttccgct aatcagacgc tacgccagcg gactaccgaa gcggtaatgc
     8701 gcctgcttag cgggcaaaaa gaagatgcgg aactggacgc cgaaaccgcg tccatgctgg
     8761 tggatcatgg aaaccagcag atctttaatc cgcaggaacg gcggatgatt gagcgggtac
     8821 ttaatcttaa ccagcgtacc gtcagcagca ttatgacgtc gcgccacgat attgagcata
     8881 tcgatctcaa cgcgccggaa gaagaaatcc gccagttgct ggagcgaaat cagcatacgc
     8941 ggctggtggt taccgacggt gatgacgcag aagatttgct cggtgttgtt cacgttatcg
     9001 acctgctaca acagtcactg cgcggcgaac cgctcaacct gcgggtgttg attcgccagc
     9061 cgctggtgtt cccggaaacc ttgccgttgt taccagccct ggagcagttc cgtaatgccc
     9121 gcacgcactt tgcttttgtg gtggatgagt ttggctcggt ggaagggatt gtgacattaa
     9181 gtgacgtcac tgaaaccatt gccggtaact taccgaacga agtggaagag atcgacgccc
     9241 gccatgatat tcagaagaat gcggacggtt cctggacggc gaatggtcat atgccgctgg
     9301 aggatctggt gcaatatgtg ccgctgccgc tggatgaaaa acgtgaatat cacaccattg
     9361 ccgggctgtt gatggagtat ttgcagcgta ttccaaaacc tggcgaagaa gttcaggtgg
     9421 gggattattt gcttaaaacg ttgcaggtgg aaagccatcg cgtgcagaag gtgcagatta
     9481 taccgctgcg taaggatggc gagatggagt acgaggtgtg atggtatcgg ccattttacc
     9541 caatgctgcg taacgcctta taacttcagc aatgaagcgt tgtctgtggt aaacggacat
     9601 cacagcgggg gtgtgggtcc ggctgtaaat cgccgaatcg tttcctgtag gctggggcag
     9661 cccacatgga tgtgggctga gggcgcgttt tacagggatg ttacctcgcg cccgtacccg
     9721 atagccgaaa ggaataagat gagggaaccg cgccagcggc gatttaccgc cgggagcccg
     9781 ggttgccagg gtggtggcga ttgagccacc ctggcacgtt caccggcttg agcgttccat
     9841 agtagcgagg aacatcaagt gaacggaaca gccttcgatc ttacatattc agaatataat
     9901 agtgccgccg cgaacgcctg atgcgacgct ttacgcgtct tatcaggcct acaggacagg
     9961 tcacctggta acctacatct tctccagcaa cttcttcaca tctttgccat tgcgggaatc
    10021 tttattccgc tcggcccagt cattcaagcg acgtttcgct tcgtcctgta gatgtttgcg
    10081 cagtaactga tccacttgca ggctgtaatt gagttgctgc cagttgccat aaacccgcag
    10141 cggtactggc gtttctttga ggaaatcaat cagtttgctt tccccgttcc agccacccac
    10201 gacccgaata tcaaactggg tgtcgcaggt ttgatctgcc agattcaaca tgccttcccc
    10261 tgtcagcgcc agcacaggcg attgaccttg catgtcgttt aacgtcacga cgccatcctt
    10321 caacgtcaaa tcggtggtaa agcggtcaag ccgcgtcacg ttatcgaagt tttcagcggc
    10381 cttcacatca ccgccattac gctctaccgc ttgctgaatc atctgctgga agttcatccc
    10441 ttccatacgt gtgtcggtca tctcaacatg cgcctgccct tgccagttgt ggcggaatgc
    10501 gtcggcatct atgtcagcac cggagaaatc accagccagt gacatttttc cggtcaacga
    10561 aatcggatag ttaaacgcct tcagaatggt accaatctca acgttctcca gccgtggctg
    10621 gaagtttatc cgcggattta ttgatgtcgc gtccagcgtg cccggcagtg aaacctttcc
    10681 accgttaagc ttgccctgca gttgagtaat ttccagcaaa cccgacttgt tggtcatttg
    10741 cgtggcaaca tctgtaaaat tcattccgcg ccagcgcacg ttactggcct gcaacaaaat
    10801 atcagctgta aagccttgca gtccctgata ggccggttca tcaatacgcg aagaaatgac
    10861 cgggcgcggc aacgttgatt ggctctgccc ctgctgcgcg gcaccgtttt caccattcgc
    10921 ggtttcatta agcgggatga ggttgtcaag attcagttgc gggaattgca gtctcagctg
    10981 ccattccggt ttctctgtca gcgtgacctg tgcttgcccg ctcagcgtac tgtcattggc
    11041 ggtcaaacta atttggttaa atgaaaggcg tttatgtgac tcctgccact gggactggaa
    11101 actcccctgc ccctgaatac cttgttttgg taaatcggca ccctgcaact gccagttaat
    11161 ttgttcaata gccgctgtta aatcatgcgg ataatcagaa gcatctaccg taccgttaag
    11221 ggatatcgtg agatcgcgct gatcgcgatt aacccgcccg gagaactcaa atgagccacg
    11281 atgttgggga tcttgttcca tttgcaggcg gatattgcgg attgtcacct gctcgtcatc
    11341 ttcatgctgg aacaccagaa cgctgtccgc caccttaaga ctggatatat caaacgacca
    11401 tccgcgatcg tctgacagat ccggcaaggt attgtcgcgc ggtgcaaccg gagcgtcttc
    11461 actgcgcacc gcttccgtct gcggcgtcag ttggatcact gcccctttta gcatcacctg
    11521 cttaacgctc agttgatgac tcagtagtgg taaaagcgcc acgtccagac gcatgttgtc
    11581 ggcgcgaacc agtggctggc ttgcgccctg ggcggtgaga gacattcgcc cggagaggat
    11641 actaagctgc ggccagacgt gccaacgcag tggcccgtcg agctgcaatt gataaccgct
    11701 acgtgcagca acttgcttga ccatatagtc gcggaaatca ttcggattca ccagcaacac
    11761 taacgcagat aacccggcca ccagcacgac caggagtatc atcagcgtcg tcagaaatcg
    11821 tctcatggta tcctcaatgg gcctgaatta gtctttatcg attcggctgg ctaccgcgcc
    11881 ctgctggttg cgatatttcg catcttcacg gcggttgtaa ggtcgcgccg ccgggccgga
    11941 aagcggctca aagctcagcg caccaattaa catgcccgga cgcagcgcca gcggcagctt
    12001 accggagttg tagaactcca gcacaatgca accagaccag cccggatcga tacggtgcgc
    12061 ggtgacgtgc accatcagcc ccagacgcgc caaagaggaa cgcccgtcca gccagcccac
    12121 cagatcggct ggcagcgtca ccgactccag cgtcaccgcc agcgccagct ctcctgggtg
    12181 aagatagaac gcctcgccct cgtcgagaac gatctcatcg ctcatcacgc ggtcaagcgc
    12241 ggcgctcact tcatctttgg gaccgctcag atcgataaac gctgccgtgt gaccacggaa
    12301 ggtacgaaat ttattgccca ggcgtacatc caccgtcgcg ccgttaatac gctccactgg
    12361 cggacgtggg ttgatcgaca aacggccttc atcaagccag gcttcaatat ctcggtcaca
    12421 cagacgcatg gcattttctc ctttcgcgca tcactccctt aacgccaatt acgtcaaggg
    12481 cataccagct tatcactgaa cggtacacaa tttatcaagc ttattcaaag aactgactta
    12541 ttttcgcttt caatatatcg atcgcgatgc ggtttttccc gccgcgcggc acgataatgt
    12601 ccgcgtattg tttagaaggc tcaatgaatt gcaggaacat cgggcgcacg gttttttgat
    12661 attgcgccat cacggaatcc attgaacgcc cacgctcgtt aacgtcacgc ttgatgcggc
    12721 gcatcaggca gatatccagc ggggtatcaa cgaaaatgga gaagttaagt tcgtcacgca
    12781 aacgcgcatc cgtcagcaac aaaatgcctt cgagaatgat gaccttcttc ggctcaacct
    12841 tcaccgtttc tttcatacgc gtatgttcaa catagctgta aaccggcagg tcaattgccg
    12901 agccgcgttt caacgcttgt aaatgctcaa gcaacaggct gtgatccatc gcgctgggat
    12961 ggtcgtagtt ggtcttaacg cgttcttcca tcgacagatg gctttgatct ttgtaatagc
    13021 agtcttcggg aattacgccg atgtgttcat caccgacttg ctcacgtaat tcacgataaa
    13081 gggtactggc aataagactc ttgccggaag ccgatgcgcc agcgataccg ataatgacgc
    13141 actgatgaga ctgatcagtc atatatttag cgacctgatt aacctggatg ttaggaaggg
    13201 ggcgacgaag cgccaaacgc ggcaattata gggatttcat ccgtctgata ccagtcgaat
    13261 agcgttgccg cgcgctcaga gttaattgtt gacaaagaat tcccggtggc aaattacgtt
    13321 gatcagtttt atgtaaggta aaaaatgtta tacgcagttg cgcaaattat ccgcctttac
    13381 gtcactttat gagcaattcg catataaaat gtaaaacttt tgtactagca taaacacaga
    13441 aacgaatact ggcgacctgg ccttgcggat aaagcggtaa taagcaaaca atcacagcat
    13501 gtattaattg ccctgcccca cccgctgctt cacctggtca gtttaggttt agtctcgttt
    13561 atctttaccc ttgtctcgct tgagctttcg cagtttggca cccaactcgc cccactgtgg
    13621 ttcccgacgt ccatcatgat ggtggcgttt tatcgccatg ccgggcgcat gtggccggga
    13681 attgcgctga gctgttcgct gggaaatatc gccgcatcca tcctgctttt ttccaccagc
    13741 tcgctgaaca tgacctggac gaccatcaat attgttgaag ccgtggtcgg ggcagtgctg
    13801 ctacgtaaat tgctgccgtg gtataacccc ttgcaaaatc tggctgactg gctgcgtctg
    13861 gcattcggca gcgccattgt tccgcctctg
//
LOCUS       DAJTYG010000093        13829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTYG010000093 DAJTYG010000000
VERSION     DAJTYG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.093"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            166..1419
                     /gene="kch"
                     /locus_tag="OO568_003082"
     CDS             166..1419
                     /gene="kch"
                     /locus_tag="OO568_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6642352.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1474..1647)
                     /locus_tag="OO568_003083"
     CDS             complement(1474..1647)
                     /locus_tag="OO568_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6642353.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1790..3250
                     /gene="cls"
                     /locus_tag="OO568_003084"
     CDS             1790..3250
                     /gene="cls"
                     /locus_tag="OO568_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6642354.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3285..3614
                     /locus_tag="OO568_003085"
     CDS             3285..3614
                     /locus_tag="OO568_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6642355.1"
                     /translation="MDMDLNNRLTEDETLKQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3667..4671)
                     /gene="oppF"
                     /locus_tag="OO568_003086"
     CDS             complement(3667..4671)
                     /gene="oppF"
                     /locus_tag="OO568_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6642356.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4668..5681)
                     /gene="oppD"
                     /locus_tag="OO568_003087"
     CDS             complement(4668..5681)
                     /gene="oppD"
                     /locus_tag="OO568_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6642357.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLRACFKPVE
                     ELL"
     gene            complement(5693..6601)
                     /gene="oppC"
                     /locus_tag="OO568_003088"
     CDS             complement(5693..6601)
                     /gene="oppC"
                     /locus_tag="OO568_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6642358.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6616..7536)
                     /gene="oppB"
                     /locus_tag="OO568_003089"
     CDS             complement(6616..7536)
                     /gene="oppB"
                     /locus_tag="OO568_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6642359.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7622..9253)
                     /gene="oppA"
                     /locus_tag="OO568_003090"
     CDS             complement(7622..9253)
                     /gene="oppA"
                     /locus_tag="OO568_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6642360.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(9991..10638)
                     /gene="ychE"
                     /locus_tag="OO568_003091"
     CDS             complement(9991..10638)
                     /gene="ychE"
                     /locus_tag="OO568_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6642361.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11115..13790
                     /gene="adhE"
                     /locus_tag="OO568_003092"
                     /gene_synonym="adhC"
     CDS             11115..13790
                     /gene="adhE"
                     /locus_tag="OO568_003092"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6642362.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3628 a         3426 c         3223 g         3552 t
ORIGIN      
        1 gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt tgattactgc
       61 ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt tcagaaaatg
      121 tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag tcactgggct
      181 acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat tctcgcgctg
      241 gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc ggtcaatttg
      301 cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct ggcaaacgcc
      361 ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact gttccgggca
      421 aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta caccctgcat
      481 ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt gtttttgctg
      541 atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt tgcatttatt
      601 agtttcacga cattactgtt ttactcaacc tacggtgcac tttatttaag cgaaggtttt
      661 aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac catgtcaacc
      721 gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac tatttcggtc
      781 attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg tccgcttatc
      841 cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg taaagatcat
      901 tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact gaatcaacgc
      961 ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca acttgagcaa
     1021 cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc agtattaaag
     1081 aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga tgcagataac
     1141 gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt tctcgccgtc
     1201 agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat tctctcaccg
     1261 caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat taataatgat
     1321 atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa cgatgaacaa
     1381 gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaacgac ggaagagtaa
     1441 acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg caaacaataa
     1501 cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca tatttcggcg
     1561 cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca tgcgccagcg
     1621 ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca gaatggtcat
     1681 tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg ccagatgaat
     1741 ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta tgacaaccgt
     1801 ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg caggcgtaac
     1861 tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc tgttgattat
     1921 ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc tccatttagg
     1981 caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat ggcttaacga
     2041 ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg cgccattatt
     2101 caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc tacaactgat
     2161 gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg cgcgccataa
     2221 tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg tggctgaatc
     2281 attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact ccgccgggag
     2341 tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta ttgaagtggt
     2401 cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc tgcgccaaca
     2461 tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga atatggtcga
     2521 tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga tggcgcgtat
     2581 ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg agattgaaac
     2641 cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg aacaggccag
     2701 tggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc tcattcacca
     2761 ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca cgccctactt
     2821 tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg gggtggatgt
     2881 cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca gtcgcgcatt
     2941 ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg ggttactgca
     3001 taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta accttgatat
     3061 gcgtagtctg tggctgaatt tcgagattac cctggcaatc gacgataaag gttttggtgc
     3121 agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg atgcccgttt
     3181 atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact tcttcagccc
     3241 gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat atggatctaa
     3301 acaatcgcct gactgaagat gaaacgctta agcaggccta cgatattttt ctcgaactgg
     3361 ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt gaagagcgcg
     3421 gcggcgcgga gttattcgat ccggcggaag actggcagga acatgttgat ttcgatttga
     3481 acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat ggtgaaatca
     3541 acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt tgccatatca
     3601 tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg tcagccctta
     3661 ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac tcccctccag
     3721 aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac ggaaaacaca
     3781 accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga tggttttgtt
     3841 cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg tgtaaggatg
     3901 tagtggattg tggtagacct catcataggt ccccagttct actgcatggc cgagatacat
     3961 caccaacaca cgatcggaaa tgtgttttac cacggctagg tcatgagcaa taaaaattaa
     4021 tgacaatccc atctcacgtt gcagctgctg gagcaggttg accacctgcg cctgaattga
     4081 cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa gaataagagc
     4141 acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat agcggttaat
     4201 caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc gaacttcctg
     4261 gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc cgatggtcat
     4321 acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac tgcgaacggc
     4381 acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa catgaccgtc
     4441 ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc atcccgattc
     4501 ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat cgacggcttt
     4561 gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa agtgcacttt
     4621 aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca taacagttcc
     4681 tccaccggtt taaagcaagc acgcagacgg ccaagcgtaa actcttccag cggcggtgcg
     4741 ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc tttcggtaat
     4801 cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc cgcatcaaga
     4861 cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata aaagacatcg
     4921 cgcgcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt gtcacagatc
     4981 cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa ttcccgcttc
     5041 agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc tgtagtgggt
     5101 tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat gactcgctga
     5161 cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc ttccggcatt
     5221 tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct catgttctta
     5281 tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt caacgaagtc
     5341 attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag ttcacgctct
     5401 ggcaaattga ggatttcacg cccattgaag gttgccgatc cgccaatgcg tccgttggca
     5461 gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga ctcacctaca
     5521 atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc cgtgacgtcg
     5581 ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc gtcagcctgt
     5641 tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact ccttaacgat
     5701 ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa aacagtgtca
     5761 ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc gagttcgcgc
     5821 catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac cccaggaagc
     5881 taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag accaccacca
     5941 caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat acaccgccaa
     6001 cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca cgcacaatac
     6061 gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg atgttttggc
     6121 cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg aggatttcca
     6181 gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc gaaccataaa
     6241 gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt gagatacgcc
     6301 cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca aagtagtgac
     6361 cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca taggcaaact
     6421 gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact atcagactgg
     6481 caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag ctgcgccctt
     6541 cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta cttaacatca
     6601 tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata gcacatcgac
     6661 aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta acactaagga
     6721 atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca aaccataaat
     6781 ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca tataggagag
     6841 cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca taggtaaccc
     6901 tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag agccacgggt
     6961 aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca atatcatgaa
     7021 tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga tcgcaaatat
     7081 catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca ttgccagccc
     7141 catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc cagcactaac
     7201 acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa cgggaaaact
     7261 ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac cgaaatcgcc
     7321 atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat taagatgata
     7381 tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg taaaagggct
     7441 tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata gcgtcggaat
     7501 cgcttccaga cagcgacgta gaataaattt taacattgcc ctaccttctg gcctgtgcct
     7561 tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc acgtattgcc
     7621 attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga tctttgccgg
     7681 tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa acaggaacaa
     7741 tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg cgctgcgcct
     7801 cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc ttataatgcg
     7861 cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt tcgttgtagt
     7921 ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg tcgaggaacg
     7981 ttttccactc ctggttaacc agtttgacgt ttacaccaat gtttttcttc cacaatgaag
     8041 aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg ttgatggtca
     8101 acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct tcgttacgtt
     8161 tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca gtatacggtg
     8221 gagtgtaacc ataggcgggc atgtcgccct gcgctttcac tttattaaca atgatatcgc
     8281 ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc ggtttctggt
     8341 tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg tccgggatct
     8401 cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg tcgatttcac
     8461 cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag gttacctggt
     8521 taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg attcgttcgt
     8581 ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca ggctgggtcc
     8641 atttttcgcc gaatttctcg atagcggctt ttggcaccgg tgaagttgat gggtgaacaa
     8701 gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg tgatcatcaa
     8761 tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca tcaataccgg
     8821 cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga tcaacagaac
     8881 gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac catttcgcat
     8941 ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat tcagcgacgc
     9001 caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg tctcggctga
     9061 tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact tctgaaccat
     9121 tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca tcagctgcca
     9181 gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa cttctcttgg
     9241 tgatgttggt cattgttttt tggactccct cattataatt actggtatta accagcatgt
     9301 gtaatcccgg attgggaccc tgcacaggct cggagacttt ctgctgtcag gttactttat
     9361 cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat caccttatgt
     9421 cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc tctttaattg
     9481 ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag taccaaatgc
     9541 ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt attgaccgta
     9601 tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa tcaaatagag
     9661 attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg cacaataatt
     9721 taacaattga ttattattta gcactttcct ctaccaaagg tagtggaatt actaatatca
     9781 tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca tgatggtgag
     9841 gagaagcagt taaggaaatc cttataacta actgtaattt gagaagaata tttttaccta
     9901 ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga agtaaacgga
     9961 gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga aaatcccctt
    10021 aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca taatacgcgt
    10081 aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca tgcggaacaa
    10141 tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca gatagctaat
    10201 gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc ccgccatcaa
    10261 cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg atttttcttg
    10321 cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta tcgtcaccac
    10381 caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt gtagaatcgt
    10441 gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg ctgtaaggtt
    10501 agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga cgggaataat
    10561 ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa cgggaaaatc
    10621 aaaaagggtc tgaatcacgg ttagctccga agcaaaagcc ggataatgtt agccataaat
    10681 aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc tcctaactac
    10741 ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta atgtagccac
    10801 caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat gctgaaaggt
    10861 gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt gagtaatctt
    10921 gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta aactctttta
    10981 gtaaatcaca gtgagtgtga gcgcgaataa acttttgatt ttcattggtt aagcaaatca
    11041 tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa gtttaacatt
    11101 atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc gtagagcgtg
    11161 taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac aaaatcttcc
    11221 gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg gccgttgccg
    11281 aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct tctgaatata
    11341 tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac gacacttttg
    11401 gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg accactaacc
    11461 cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac gccattatct
    11521 tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc gttctgcagg
    11581 ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa ccttctgttg
    11641 aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg actggtggtc
    11701 cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta ggcgcgggta
    11761 acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca tctgtactga
    11821 tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt gttgttgttg
    11881 actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat ctgttgcagg
    11941 gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg aacgcggcta
    12001 tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta ccagaaaaca
    12061 ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc gcacatgaaa
    12121 aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg gtagaaaaag
    12181 cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac actgaccagg
    12241 ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct cgtatcctga
    12301 ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa ctcgcacctt
    12361 ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac gttggtccga
    12421 aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg tggcacaaac
    12481 ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat gaagtgatta
    12541 ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac aatggttatg
    12601 ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc ttcttcgaag
    12661 tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc agaactggca aactccttca
    12721 aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg aagatcatgt
    12781 gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc tttatggata
    12841 tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg atcgctgtca
    12901 ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact gacgacgcta
    12961 ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg attgtcgacg
    13021 ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg gacgcagtaa
    13081 ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat ggtcaggctc
    13141 tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa gggtctaaaa
    13201 atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc gcatttgcga
    13261 acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag ttccatattc
    13321 cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat gcgaacgaca
    13381 acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc cgtcgttatg
    13441 ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct gctaagatcg
    13501 agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg aaatctatcc
    13561 gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg tctgaagatg
    13621 cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc gagctgaaac
    13681 agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact gcagcgaaaa
    13741 aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa tcagtagcgc
    13801 tgtctggcaa tataaacggc cccttctgg
//
LOCUS       DAJTYG010000094        13604 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTYG010000094 DAJTYG010000000
VERSION     DAJTYG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.094"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(99..1178)
                     /locus_tag="OO568_003093"
                     /pseudo
     CDS             complement(99..1178)
                     /locus_tag="OO568_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
     gene            complement(1388..1831)
                     /locus_tag="OO568_003094"
     CDS             complement(1388..1831)
                     /locus_tag="OO568_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642363.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLPRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFFLTR"
     gene            2346..3188
                     /locus_tag="OO568_003095"
     CDS             2346..3188
                     /locus_tag="OO568_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642364.1"
                     /translation="MKKELFGRCPFDLDEDVKKIAAINKYIHAKFEEHKNKIDNLKGV
                     EQKLMVLQYFNVLNELSNQAISSLNIGAFSASEVLARVIIEQAANQFYIAIDDGENAQ
                     ALLKASKKLVHSNGKRWLECLKSKGMTNPAANERIRMGKELTDLFNRLWPNTPEYPGT
                     RKLFELIGWETHYHAHYVPLCDSIHTFSDDMVNIVSLYNAIQSDETTAIEFILAVKQE
                     NKRLAIYNFVIATALRCEALVNVFNSLGYQDIITEMTPTIDTVNQTIIRYDDFDHSRI
                     DDCQ"
     gene            3295..3558
                     /locus_tag="OO568_003096"
                     /pseudo
     CDS             3295..3558
                     /locus_tag="OO568_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            4185..4730
                     /gene="cobU"
                     /locus_tag="OO568_003097"
     CDS             4185..4730
                     /gene="cobU"
                     /locus_tag="OO568_003097"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6642365.1"
                     /translation="MMILVTGGAWSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            4727..5470
                     /gene="cobS"
                     /locus_tag="OO568_003098"
     CDS             4727..5470
                     /gene="cobS"
                     /locus_tag="OO568_003098"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6642366.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSCDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            5482..6561
                     /gene="cobT"
                     /locus_tag="OO568_003099"
     CDS             5482..6561
                     /gene="cobT"
                     /locus_tag="OO568_003099"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6642367.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            6623..7558
                     /gene="ldtA"
                     /locus_tag="OO568_003100"
     CDS             6623..7558
                     /gene="ldtA"
                     /locus_tag="OO568_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6642368.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFASQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(7614..7689)
                     /locus_tag="OO568_003101"
     tRNA            complement(7614..7689)
                     /locus_tag="OO568_003101"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7654..7656),aa:Asn,seq:gtt)
     gene            8016..8933
                     /gene="nac"
                     /locus_tag="OO568_003102"
     CDS             8016..8933
                     /gene="nac"
                     /locus_tag="OO568_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6642369.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            9035..9985
                     /gene="cbl"
                     /locus_tag="OO568_003103"
     CDS             9035..9985
                     /gene="cbl"
                     /locus_tag="OO568_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6642370.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(10023..10098)
                     /locus_tag="OO568_003104"
     tRNA            complement(10023..10098)
                     /locus_tag="OO568_003104"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10063..10065),aa:Asn,seq:gtt)
     gene            10103..11730
                     /gene="yeeO"
                     /locus_tag="OO568_003105"
                     /pseudo
     CDS             10103..11730
                     /gene="yeeO"
                     /locus_tag="OO568_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            11831..11906
                     /locus_tag="OO568_003106"
     tRNA            11831..11906
                     /locus_tag="OO568_003106"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11864..11866,aa:Asn,seq:gtt)
     gene            complement(12362..13078)
                     /locus_tag="OO568_003107"
     CDS             complement(12362..13078)
                     /locus_tag="OO568_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6642371.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(13421..>13604)
                     /locus_tag="OO568_003108"
     CDS             complement(13421..>13604)
                     /locus_tag="OO568_003108"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6642372.1"
                     /translation="CVSDKPLHGEIKLPGQANRFYEGAISEHLQIGIRAIDLLRAEGD
                     RLHSRKLRTFNEPPFR"
BASE COUNT         3479 a         2943 c         3369 g         3813 t
ORIGIN      
        1 cacaggtctg tcgccccttc agttgccagt tcgctgtctt tacagacgta atcttttttc
       61 atgcgaccga aggcttccct gacaaaatca tcgacctagg cttcatcaac aggaagggtt
      121 ccgtttttcc gtaactcatg aagcgcacgg ttagccaggt atgcctggcg tgaggatccg
      181 ggataaacgc gggaggcggg cagcacatcg ctgttcagca ttgccggtac cttctgacag
      241 actgcatcag ccccatcgcc attacggtct ttttcatcaa atttattgac cagcacaaac
      301 aggcggccga ctgacacatc agcaatggca ttaagctctt tacgaacctc ctcatcggct
      361 ttcgagttca tctgggtgta gtccatcacc gccagcaccg cagaggcttt ctgcagttga
      421 tcccgcatca tcacttccat ctgcggttgt ccggcttcat tagggccggg ggtatccacc
      481 agtgtgagtg ttccctggca ttcatccatc cccaccagat ggctgaattc cacgtcgatc
      541 accggcagtt tctgcacttc tgcgtattca tcaaaaggaa attcactccc cattgccgca
      601 gcaagacgaa ccagatcgtt taacgatgcc agtccggtaa agattccctc ctccccgtgg
      661 tattcatttt tgagccagcc atcatccgtc agaatatcca gcagttcgcg ggtatccccg
      721 gtttgctgca gggtctgtgc gacctgctgt cctgccgggg tggcgacttt ttcctgcagt
      781 gtgattaata aattgcggac aggctgaata tgttccagat ggagaaccgg ctcagttttt
      841 ccgggaacgt ggcggatgag cgtcggtaca gaggtcatgg ggcggttacg gttaggcaga
      901 atttcctgcc ccacaatggc gttgatggtg gttgatttgc ctgctttcat cgtgccgaca
      961 acagccagca ccatctccct gcgggcggtt ttgatttgtt cgccctccag ctccttaatt
     1021 cttttcagtg ctttctgttt atcaaaaagc tgcccgtttt cgttcttacc gtcagataac
     1081 acatccggct catcaaccat ttgccggagc agattgatgc tcagttgcaa aagccggtcg
     1141 gcttcatcac aaagcagggt gatgttcttt tcgtgcatta tttaaccttc ctgagacgca
     1201 ttttcatgac aaccacggag gaggccattt actacaagcg atcgtttata acgatcaata
     1261 taatagaaac gatagtcata ttctattaat atgaaaaaca caacgcaagg gggacaaccc
     1321 gggtatggct gcgtttgcat tgtgttttca tagagttaaa aatctgtaac gacaacccgg
     1381 cacatcacta ccgggttaag aagaaaatcc agttccatac cgcgcgggcc acagagacca
     1441 cggtgtcacg aacggcgcgc agggccgtac ggacaacaga ggcaacacag acactctccg
     1501 ccgtgtcaaa tatccggtcc accgcaccgg taaactgttc acgggtctga gtgcggacag
     1561 actccgtgcg cagttcgtcc tgcaggcgtg tcatcagagg gatggcggca tgtccgggaa
     1621 gcactgtcat gagtgcactg accggggtga cctccccctg aaacagtgca ttacagagtg
     1681 aggatttacc ggccccgctt ttacccataa tgccaggcac gggttcgtga cgggggagtt
     1741 tgcgcagatg ttccaggatg tgacgggaaa tcgggtaagg cagggaggag agcggttttt
     1801 caattgcctc aatggcatca gacggattca tacatgtatt cccagtgaaa aaagacaaaa
     1861 ccccgtgcac cggaaaggtg gcgggggatt cacggagata atatctttct gaaattattt
     1921 tgaatgcagg tatatgtggc tgatttgtat cagctaatga gtacaggtct gaatctaatc
     1981 tgacagtccg ctttgtgcca ggagcggact gacctgctcc ccgatgatta atacacagct
     2041 atgctagtaa tgtctgtaga tcgctgtgtg ttgcatctat cggttgaact cacagtccaa
     2101 accagactgt cagctttgat tagattctac ctactcaacc tgtcagataa ggtctgagct
     2161 aatacatcta atatatggct ttgtgcaggg gacggctatc gcgaacgtta ggctaggtat
     2221 ctgaatacgc acagttcact tggttgtact tcttgcactg atctgctatc ccgcacagct
     2281 atttataggt aatcttcagt gtataatctg ccgacttatg gaagcttgta caacaggaga
     2341 aatatatgaa aaaagaattg tttggacgtt gcccgtttga tctcgacgaa gatgtaaaaa
     2401 aaatagccgc gataaataaa tatatccatg caaaatttga ggagcataaa aataaaatag
     2461 acaacttaaa gggtgtggaa caaaaattga tggtcttaca gtactttaat gtactgaatg
     2521 aacttagtaa tcaggctatc tcttctttga atataggagc cttttcagca tcggaagtat
     2581 tagctagggt tataatagaa caggctgcca atcaatttta tatagcaata gatgatggcg
     2641 aaaatgcaca agcattactc aaggctagta agaaattagt tcatagtaat ggaaaacggt
     2701 ggctggagtg tttaaaatcc aaaggaatga ctaatccagc agcaaatgaa aggattcgca
     2761 tgggaaaaga actaactgat ctatttaata gattgtggcc aaatacacct gaatatccag
     2821 gaaccagaaa actttttgaa ttaattggct gggaaacaca ctatcatgcc cattacgttc
     2881 ccctttgtga ttcaatacat acattctctg atgatatggt aaatatagta tctctatata
     2941 atgcaatcca aagtgatgaa actactgcta tcgaattcat tcttgcagtt aaacaagaaa
     3001 ataaacggct ggcaatttat aattttgtaa ttgctacggc ccttcggtgt gaggccttag
     3061 taaatgtatt taactcttta ggttatcagg atattataac agaaatgaca ccaacaatag
     3121 atacagtcaa ccagactata attcgatatg atgattttga tcattccaga attgatgatt
     3181 gtcaataatt aattgacaga atacgtacat gcgcaacaac tagtgatagt tgacttattt
     3241 tcgctcttga gagacaacca tactcaaatc tcccacattg caggagattt gaatatgaaa
     3301 acgtcaccgt ggaataaaga ccgtaccatc ggtcaaaaaa ggccacttca gatatctcat
     3361 atctggggga tccggattag acttgagttg gaaggtaaaa cacgcgatct agctctgttc
     3421 aacatggctt tggacagtaa gctacggggc tgtgatttgg tcaaactcaa agtatcagat
     3481 gctgcatatg gcagttctgt ctcaagcaga gcaacggtgt tgcaacagaa aaccggcagc
     3541 cctgtccaat ttgagatgcc tttaataaca catcttttaa atgttgaaga attatcccgc
     3601 ctgctgaaga acgttgctct gagtgtgata aaccttttta ttcttgatga tgtcagcgag
     3661 aagatggaat atatccccga ctgggtcgtg gttacccggt taaacatctg gtggcgctaa
     3721 taaatctggc aagtcacatt ctgttgaatg ctgtgacagt gccttaccga gagagcgaaa
     3781 ttgtggtgtt ctagtgcata gtcgccacca ttccggataa ttctattgcg ttgttttaca
     3841 agttctttta cagcgcagat ctgtcgctga cgttgaatca atagcctgga tttcagaaca
     3901 caatgcccgt ttgctggttg gtccggcagt agcattaatt cattatctga atgaaatgtg
     3961 tcaatccaca gaaaccgctg tcaatgacgc attttgtcgg acgttaacac gagaaagtct
     4021 ggctattccg taatgccatc ttatttcgta atggattgag aatgctggat ttttaattat
     4081 attacgcgca gtattaatcg cgtaaatgtt ttgcggtcag caacggtggg ggatccattg
     4141 ctctgttcaa tgaataccat catacatttg aatcaggagt cggtatgatg attttggtga
     4201 cgggaggcgc atggagcggg aagagtcgcc atgcagaggt gcttattggg gactcttcac
     4261 aggttctgta tatcgctacc tcgcaaatcc ttgatgatga gatggctgca cggatagaac
     4321 atcatcggca aagccgcccg gagcactggc gcacagtgga gcgctggcaa catcttgatg
     4381 aattaattca tgcagacatt aacccgaatg aggctgtgtt gctggaatgc gttaccacaa
     4441 tggtgactaa tctgttgttt gattatggcg gcgataaaga ccctgatgaa tgggattatc
     4501 aggcgatgga acaggcgatt aatgctgaga ttcagtcgtt gattgctgcc tgccaacgtt
     4561 gccccgcaaa ggttgtatta gtgactaacg aagtgggaat ggggattgtg ccggagagtc
     4621 gtctggcacg acattttcgt gatattgccg ggcgggtaaa tcagcagttg gccgctgcgg
     4681 caaatgaagt atggctggtg gtttcgggta ttggagtaaa aatcaaatga gcaaattatt
     4741 ttgggcgatg ctctcattta ttacgcgtct gcctgtaccg cgtcgctggt cccagggact
     4801 ggatttcgag cattattctc gcggtattat tacttttcct ttgattggat tattgcttgg
     4861 cgcgattagc gggctggtct tcatggtgct gcaggcatgg tgtggcgcac cactggcggc
     4921 actgtttagc gtacttgtgc tggcgctgat gaccggggaa ttccatctgg acggcctcgc
     4981 tgatacctgt gatggcgtat tttctgcacg tagctgcgat cgcatgttgg aaatcatgcg
     5041 tgatagccgt ttaggcaccc acggcggtct ggcattgatt tttgtggtac tggcaaagat
     5101 tctggtgtta agcgagttgg ctctgcgtgg cgaaccgatc cttgcatcgc tggcggcagc
     5161 atgtgcggtc agccgcggga ctgccgcatt attgatgtat cgtcatcgtt atgcgcggga
     5221 agaagggctt ggcaatgtgt tcattggcaa aattgatggg cgacaaacct gcgtcacact
     5281 cggcttagcc gctatttttg ctgccgtatt attgctgggt atgcatggtg tggctgctat
     5341 ggtggtgacg atggtggcga ttttcatcct cggtcagctt ctcaaacgta cgctgggcgg
     5401 gcaaacgggc gatacgctgg gagcagccat agagcttggt gaactggtct tcctgctggc
     5461 actgctataa agagacatga catgcaaata attgccgatt tactgaatac gatcccagcc
     5521 atcaattctg ccgctatgtc gcgtgcacaa cggcatgttg acgggttact caaacctgtt
     5581 ggtagcctag gaaagctgga ggcgcttgcc atacaactgg caggaatgcc gggggtgaat
     5641 ggcataccgc atgtgggcaa aaaagcggta ctggttatgt gtgccgatca cggcgtctgg
     5701 gaggaagggg tcgctatttc tccaaaagaa gtgacagcca tacaggctga aaatatgacc
     5761 cgtggaacaa ccggcgtgtg tgtgctggca gcacaagcgg gcgctaacgt ccacgtaatt
     5821 gatgttggta ttgatactgc tgagcctatc cccgggctta tcaacatgcg tgtcgcacga
     5881 ggtagcggca atattgcttc agctccggca atgagtcgcc gtcaggctga aaagttgctt
     5941 ttggacgtca tatgttatac gcgggagctg gcgaaaaacg gtgtcacgct gtttggtgta
     6001 ggtgaactgg ggatggcaaa cacgacaccg gcagcggcaa tagtcagcac aatcactggc
     6061 cgggatcctg aagaagtggt tgggattggc gcaaacctgc cgacagataa actggctaat
     6121 aaaattgatg ttgtgcgtcg ggcgattacg ttgaatcaac caaatcctca ggatggtgtt
     6181 gatgtcctgg caaaagtggg tggatttgat ttggtcggaa tagctggagt gatgttaggt
     6241 gctgcttcct gcggtttacc cgtgttgctg gatggatttc tttcttatgc tgctgcgctc
     6301 gcagcctgcc agatgtctcc tgcaatcaaa ccgtatctca ttccttctca cttgtcggca
     6361 gaaaaaggcg cgcgtatagc gctctcgcat ttggggctgg agccttatct caatatggat
     6421 atgcgtttag gtgaggggag tggtgcagct ctggcgatgt ccatcatcga agctgcttgt
     6481 gcgatataca acaacatggg cgaacttgct gccagtaata ttgttctacc ggggaatacg
     6541 acttctgatt tgaacagcta aaaacgaaaa gtctgataaa agctatactt atcggatagt
     6601 attcgcgact taggaaaggg acatgatgcg tcgtgtaaat attctttgct catttgctct
     6661 gctttttgcc agccaaaata gcctggcggt aacttatcca ttacctccag agggtagccg
     6721 tttagtgggg cagtcgctta ctgtaactgt tcctgatcac aatactcagc cgctggagac
     6781 ttttgcctca caatacgggc aagggttaag taatatgctg gaagcgaatc cgggcgctga
     6841 tgttttttta ccgaagcctg gctcacaact caccattccg cagcaactga ttttgcccgc
     6901 cactgttcgt aaagggattg tggttaacgt cgctgaaatg cgtctttatt actatccgcc
     6961 agacagtaat acagtggaag tctttcctat tggtatcggc caggctgggc gagaaacccc
     7021 gcgtaactgg gtgactaccg ttgaacgtaa acaagaagca ccaacctgga cgccaacgcc
     7081 gaacacccgg cgcgaatatg cgaaacgagg ggagagtttg cccgcatttg ttcctgcggg
     7141 gcccgataac cccatggggc tgtacgcgat ttatattggc aggttgtatg ccatccacgg
     7201 taccaatgcc aattttggta ttgggctccg tgtaagtcag ggctgtattc gtctgcgtaa
     7261 tgacgatatc aaatatctgt ttgataatgt tcctgtaggc actcgtgtac aaattattga
     7321 tcagccagtg aaatacacaa cggaaccgga tggttcaaag tggctggaag ttcatgagcc
     7381 gctgtcgcgc aatcgtgcag aatatgagtc tgaccgaaaa gtgccattgc cggtaacccc
     7441 atctttgcgg gcgtttatca acgggcaaga agttgatgta aatcgcgcaa atgctgcgtt
     7501 gcaacatcga tcgggaatgc ctgtgcaaat tagttctggt tcaagacaga tgttttaaga
     7561 gcgttggtaa ttagaaagta aaaagcctgc tcgaaagcag gcttttttga atttggctcc
     7621 tctgactgga ctcgaaccag tgacatacgg attaacagtc cgccgttcta ccgactgaac
     7681 tacagaggaa tcgtgtgaac ggggcgcata ttaatggcag tgcatggtga tgtcaaaggg
     7741 tgaaatgcat ttttttgttt gtttgctgcc aatttcaaca aagtggtgag ctttcaatct
     7801 tattgggtaa tgaaccatcg tggtgcatac cctcctttta tagggcaggt gaatgcgact
     7861 gctgataaaa agggccaatg acgaatctat cataatctct tatttgacag ttggttagct
     7921 tgcgcatcaa cgccaaaata gaactggcag gcatcttgca tactggttgt aagtaatggc
     7981 ggcacttggg ccgattctta aaaaccggag gcaacatgaa cttcagacgc ctgaaatact
     8041 tcgtaaaaat tgtagatatt ggtagcctga cccaggctgc tgaagtattg catatcgcac
     8101 aaccagcgct cagccagcag gttgccatac tggaaggtga gttaaatcaa cagcttttga
     8161 ttcgcacaaa acggggcgtt acgccaacag acgccggaaa aattctctat acccatgcac
     8221 gggccatttt acgtcagtgt gagcaggccc aactggcggt gcataacgtt ggtcagtcat
     8281 tatcggggca agtctcgatt ggctttgcgc caggaaccgc tgcgtcatcc atcaccatgc
     8341 ccttattaca ggcggttcgc gctgaattcc cggaggtcgt tatctatctt catgaaaata
     8401 gtggcgcagt gcttaacgaa aaactgataa atcaccaact cgatatggcg gtgatttatg
     8461 agcattcccc tgtggctggg gtatccagtc aggctttgct gaaagaagat cttttcctgg
     8521 taggaactca agattgcccg gggcaaaacg ttgatgttaa tgctattgcg caaatgaacc
     8581 tttttctccc cagtgattac agcgctgtta gacttcgtgt tgatgaggct ttttccctac
     8641 ggcggctcac ggcaaaagtc attggtgaaa ttgagtctat tgccacgctt accgcagcga
     8701 ttgccagcgg catgggcgtt gcagtattac ctgaatcggc tgcgcgttcg ttatgtggcg
     8761 cagtaaatgg gtggatgtca cgcattacca ctccttcaat gagtctctct ttgtccttaa
     8821 atttacccgc cagagcaaac ttgtcaccac aggcacaggc agtgaaagag ttgttaatgt
     8881 cagtgattag ttctccagtg atggaaaaaa ggcagtggca attggtgagc taagcgttat
     8941 atcgggatgg aataagatgc gggtttttat tatttgttat gccgggcatt agactttaac
     9001 aataacggga tatctgaact gcccggagtt taccgtgaat ttccaacaac taaagataat
     9061 ccgcgaggct gcacgtcagg attacaacct gacagaggtt gcgaatatgc tttttacctc
     9121 acagtcaggc gttagccgtc atattcggga actggaggat gaacttggca tcgaaatatt
     9181 tgttcgacga ggtaagcgac tgctggggat gactgaaccg ggcaaagcat tactggtcat
     9241 tgcagaacgt attctgaatg aagccagtaa tgttcgtcgg cttgcagacc tgtttaccaa
     9301 cgatacgtct ggtgttctca ctattgcaac gacgcatact caggcacgtt atagcttgcc
     9361 agaggtcatt aaagcttttc gcgaactttt cccggaggtt cggctcgagc taatccaggg
     9421 ttcgccacag gaaattgcga cgttgttgca aaatggcgaa gctgatattg gtatcgccag
     9481 cgagcgtttg agtaatgacc cgcagctcgt cgccttcccg tggttttgtt ggcaccatag
     9541 tttgcttgtt ccactcgatc atcccttaac gcaaattaca ccattgacgc tggaatcaat
     9601 agcgaagtgg ccgttaatta cttaccgaca gggaattacg gggcgctcac gcactgatga
     9661 cgcatttacc cgcaaaggtt tgccggcaga tattgtatta agtgcacagg attctgatgt
     9721 cattaaaacc tatgttgctc ttgggcttgg gatcggatta gttgccgaac aatccagcgg
     9781 cgaacaagag gaagagaatt taatccgcct ggatacgcgg catcttttcg atgctaatac
     9841 tgtctggttg ggactgaagc gaggacaact tcagcgtaac tatgtttggc gctttctgga
     9901 actttgtaat gcaggactgt cagttgagga tatcaagcgc caggtgatgg aaaacagtga
     9961 agaggaaatt gattatcaga tatagcaaaa agcccgctat aaaagcgggc ttttcagaaa
    10021 tttggctcct ctgactggac tcgaaccagt gacatacgga ttaacagtcc gccgttctac
    10081 cgactgaact acagaggaat cgctgttgag gcacatctta acggcgaaaa atattttgtc
    10141 aaacccaatt tttaaatttc ccaactgttt gccgtttcta tcaacagttt gttgcatttg
    10201 cagacaattt gttggcgaaa atctttgcag ctttgctgat tctccctcat catttgaaat
    10261 gtggtttcac tttctgcaat taaggtcggc tttgaatatc tcctctgctt tacgccaggt
    10321 tgttcacggc actcgctggc actctaaacg caagagctac aaagtgttgt tctggcgcga
    10381 gataaccccg cttgctgttc ctatcttcat ggagaacgcc tgtgtcctgt tgatgggggt
    10441 cctgagcact tttctggtca gctggctggg aaaagatgcg atggccggcg tggggttggc
    10501 ggacagcttc aatatggtca ttatggcttt ttttgcagct atcgatcttg gtactactgt
    10561 cgttgtagca tttagtctcg gtaagcggga tcgacgacga gcgagggtgg cgacgcggca
    10621 gtcattggtg atcatgacgt tgtttgccgt actgttggca acgctaattc atcattttgg
    10681 cgaacaaatt attgatttcg tcgcgggtga tgccacgaca gaagttaaag cactggcgtt
    10741 gacttatctg gagctgaccg tactcagtta tccagccgct gccatcactc ttattggtag
    10801 cggggcactt cgtggtgcag ggaatacgaa aataccgcta ctgattaacg gtagcctgaa
    10861 tattcttaat attattatta gcggcatatt gatttacggc cttttctcct ggtcggggct
    10921 gggttgggtt taaccatttc tcgttatatt ggcgcagttg caattttgtg ggtgctggcg
    10981 attggtttta atcctgcgct aaggatttcg ttaaagagct attttaaacc gctgaatttt
    11041 agcattatct gggaagtcat ggggattggt attccagcga gtgtcgaatc agtgttattt
    11101 accagtggtc ggttattaac ccaaatgttc gttgccggga tggggaccag cgttattgcc
    11161 ggaaatttta tcgcgttctc aattgcggct cttatcaact tacccggaag tgcgctcggt
    11221 tctgcttcta cgatcattac aggccgaagg ttgggggtag ggcagatagc gcaagcagag
    11281 attcagttgc ggcatgtgtt ctggctgtcc actcttggat taacggccat cgcctggcta
    11341 acggctccct ttgccggggt tatggcatcg ttttacaccc aggatccaca ggttaaacac
    11401 gtcgttgtga ttctgattta gctaaatgct ttatttatgc ctatttggtc cgcctcatgg
    11461 gtgctacccg ctggatttaa gggtgctcgt gatgcccgtt acgccatgtg ggtttctatg
    11521 ttgagcatgt ggggttgccg ggttgtagtc ggttatgtgc agggaatcat gcttggctgg
    11581 ggtgtggttg gtgtctggat gggaatgttt gcctactggg ctgtgcgggc cgtgctgttt
    11641 tactggcgaa tggttactgg acgttggctg tggaaatacc ctcgatccga gccgcaaaag
    11701 tgtgaaaaaa agccagctgt gtcggaataa acgacaaaat gcagattatt tcagcaaacg
    11761 atttcaaatt taaaaaacag gctttgacat tgtgggtggg catcgctaat attcgcctcg
    11821 ttctcacgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc cgtatgtcac
    11881 tggttcgagt ccagtcagag gagccaaatt ttagttttga gacatctcta tgagactcag
    11941 gacagttaaa aaataaaaat tatttttacc cggccacttt gtaaaaaagt ggccgggttt
    12001 tttgatttct gtgtttcgtg cgcttaatcg agtctcacgc ttcagtgagc aaattgcccc
    12061 tccccaacag acatcacgat tcacaaggtc aaaaattctg ttccatgaag taccgtacaa
    12121 agataaagag aaaaatgcct tatgcgggat gtagctgtcg cgtctttatc gggaaactat
    12181 acgtaattgc cactttgtct tcggtaatca gaaaaagaag acctgccatt ttaataaata
    12241 ttatttctct gggaacgggg attttttata agcaatatga cgagttaaac attatatttt
    12301 ttataagaat tatatgagat taaagaaatc tgccataaag acagatttct ttaaaatata
    12361 attagagatt tgcgacgtta tgataaactt tttgtacatc gtcgtcatct tcaagggcat
    12421 caacaagccc ttcaaagatt tctaaatctt ctggggaaag ctcaacttca gattgagcaa
    12481 tcatttctaa ttctgttgtt gagaactcag tgattccagc tgcttttagc gccgcgattc
    12541 ctttatgaag gtcagcaggt tcagtataaa taacaatatt accttcttct tcagttacat
    12601 cacgaacatc aacttcagct tcaagcaaaa tttcaaaaat atggtcaggg tctgttcctt
    12661 taaatacaat cacacccgta ttgtcaaaca tatagctgac agaacctgcc gctccgatat
    12721 tgccgccttt tttattgaaa attgtgcgaa cgttagcaat cgtacggtta acatttgaag
    12781 tcaatgtctc agcaataatc attgagccat taggaccaaa gccttcataa cgtccctgta
    12841 cgaacgtttc atctccgccg cctttggctt tatcaattgc tttatcaata acgtgctttg
    12901 gaacttgtgc ctgctttgca cgttcaataa cgaattttaa agatgtgttt aattctggat
    12961 cgggttcacc ttgtttggca gcagcataga tttctacacc gaattttgca taaattttag
    13021 acgttgcacc gtctttagcc gtttttttag caacaatatt ggcccattta cgtcccactg
    13081 gaatgatctc ctcgaaaatt tatttacatt actggtaaat cgttaacatg tgttaattcg
    13141 gcatattata tcataacagg aaaaatctcg tctattagaa ataaaaatat tctaaagcca
    13201 taatcttata tttaatttta taaaaactaa tgttgttggc gggatttttt ataattgtgt
    13261 tttgaaagca tacttgcttt taatatgtgg aaatcattaa gacgataata aaactcgcat
    13321 ctgcccaagg aagcaagcag cccgcgttta ttgcaggcta tttgccagat gtcttactta
    13381 atccttcaca tctatttctg ataagaaggt tcataactta ttatcggaac ggcggctcat
    13441 taaaggttcg taatttacgt gaatgcagtc ggtcgccttc tgcgcgcagc aaatcgatcg
    13501 cccgaatgcc aatttgcagg tgttcggaaa tagcgccttc ataaaaacgg ttagcctggc
    13561 cgggaagctt aatctcacca tgtaacggtt tatctgaaac acac
//
LOCUS       DAJTYG010000095        13537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTYG010000095 DAJTYG010000000
VERSION     DAJTYG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.095"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(1..892)
                     /locus_tag="OO568_003109"
                     /pseudo
     CDS             complement(1..892)
                     /locus_tag="OO568_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1133..1933
                     /locus_tag="OO568_003110"
     CDS             1133..1933
                     /locus_tag="OO568_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6642373.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2011..2781
                     /locus_tag="OO568_003111"
     CDS             2011..2781
                     /locus_tag="OO568_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6642374.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2829..4187)
                     /gene="mltD"
                     /locus_tag="OO568_003112"
     CDS             complement(2829..4187)
                     /gene="mltD"
                     /locus_tag="OO568_003112"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6642375.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4259..5014)
                     /gene="gloB"
                     /locus_tag="OO568_003113"
     CDS             complement(4259..5014)
                     /gene="gloB"
                     /locus_tag="OO568_003113"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6642376.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5048..5770
                     /locus_tag="OO568_003114"
     CDS             5048..5770
                     /locus_tag="OO568_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6642377.1"
                     /translation="MKPARVPQTVVAPDCWGDLPRGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5767..6234)
                     /gene="rnhA"
                     /locus_tag="OO568_003115"
     CDS             complement(5767..6234)
                     /gene="rnhA"
                     /locus_tag="OO568_003115"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6642378.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6299..7030
                     /gene="dnaQ"
                     /locus_tag="OO568_003116"
     CDS             6299..7030
                     /gene="dnaQ"
                     /locus_tag="OO568_003116"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6642379.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7163..7239
                     /locus_tag="OO568_003117"
     tRNA            7163..7239
                     /locus_tag="OO568_003117"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7197..7199,aa:Asp,seq:gtc)
     gene            complement(7370..8416)
                     /locus_tag="OO568_003118"
     CDS             complement(7370..8416)
                     /locus_tag="OO568_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR6642380.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8427..9422)
                     /gene="tssG"
                     /locus_tag="OO568_003119"
                     /pseudo
     CDS             complement(8427..9422)
                     /gene="tssG"
                     /locus_tag="OO568_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9419..11302)
                     /gene="tssF"
                     /locus_tag="OO568_003120"
                     /pseudo
     CDS             complement(9419..11302)
                     /gene="tssF"
                     /locus_tag="OO568_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
     gene            complement(11318..11812)
                     /gene="tssE"
                     /locus_tag="OO568_003121"
                     /pseudo
     CDS             complement(11318..11812)
                     /gene="tssE"
                     /locus_tag="OO568_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
     gene            complement(11809..12639)
                     /locus_tag="OO568_003122"
     CDS             complement(11809..12639)
                     /locus_tag="OO568_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HCR6642381.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTTLACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12626..13003)
                     /gene="tagK"
                     /locus_tag="OO568_003123"
     CDS             complement(12626..13003)
                     /gene="tagK"
                     /locus_tag="OO568_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
                     /protein_id="HCR6642382.1"
                     /translation="MLSPEQDLNRHADPFPFNEEPSSQDLDSLRDLVTEAETLQDMVT
                     GGLSIDAILNVLDATGEDETIWPVEKTPPDILHLLSPEYAPETAHNTVLPDLTRKEHR
                     IIGIDNHYRINPAQHGEIYHDKQ"
     gene            complement(13240..13410)
                     /locus_tag="OO568_003124"
     CDS             complement(13240..13410)
                     /locus_tag="OO568_003124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HCR6642383.1"
                     /translation="MNDDVKIALTLTRHEEAWWIINQSTEYCCTVNDQIVEPHHRMRL
                     NEGDLIEWGLSS"
BASE COUNT         3100 a         3523 c         3614 g         3300 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattatcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aaaattgttc cgctgcccgg ctaaagctgc
      841 cgctttcgac gaccgaaaca aaaatggcga gttcttccga cgtggctttc atttttgctc
      901 ctgttgcaaa atagatgaga tattttgaat ttatttgtca ttaaaccatc aggatgcgtg
      961 atatgtcatc cgatttaatg ttctcaataa tgagcaaatt tctgaccggt gtaagcactt
     1021 gcttacataa caatatacaa ttgctcgttg aaagagtgag ctaaaatccc tataacagta
     1081 gaaccctccc gagtgcggaa gggttgacgt aatagaggtt tcaaagtcaa aagtgcgaaa
     1141 aaacacctat gccatgcgct atgttgccgg acaacctgcg gaaaggatct taccgccggg
     1201 gtcttttgcg agcatcggcc aggcattacc acctggagaa ccgttaagta ccgaagagcg
     1261 tattcggatc ctggtgtgga acatatacaa acagcaacgc gctgaatggt tgtcggtatt
     1321 aaagaactac ggcaaagatg cacatctggt gttattgcag gaagcgcaga caacgccaga
     1381 gttagtacag tttgcgaccg ctaactatct tgccgccgat caggtgcccg ctttcgtgct
     1441 gccacaacat ccttccggtg taatgaccct ttcggcggcg catccagtgt attgctgccc
     1501 gttacgtgaa cgagaaccca ttttgcgtct ggcgaagtcg gcactggtga cggtctatcc
     1561 attacctgac acccgcctgt tgatggtggt taatatacac gccgtcaact tcagtctggg
     1621 cgtggatgtc tatagtaagc agttacttcc tattggtgat cagatagctc accacagcgg
     1681 cccggtcatt atggcgggag atttcaatgc ctggagccgt agaaggatga acgcgttata
     1741 tcgctttgcg cgggaaatgt cgctgcgcca ggtgcgtttt accgatgatc agcgccgtcg
     1801 ggcgtttggc cgcccgctcg attttgtttt ctaccgtggt ctgaacgtca gcgaagcttc
     1861 tgtattggtt acgcgcgctt ccgatcacaa tccgctactc gttgaattca gtcccggcaa
     1921 gcctgataaa taaggtatgt caggtctgcc acagggcaga ccaacgtttg gcgctgtcct
     1981 tttatactca aacaacaaaa ggacagcgca atgacaacac aatcccacca tgaccacgta
     2041 gaaaagcagt ttagctctca agcctgcgaa tatctgacca gtaccgtaca tgcatccgga
     2101 cgagatttac agcgcctggc ggtgcgtctg gctgattatc ctggtgcaag tgtacttgat
     2161 atgggctgcg gagcagggca tgccagcttt gttgctgcgc aaaacgtgag cgcggtggtg
     2221 gcgtatgact tatctgccca aatgctggat gtcgtggcac aagctgccga agcccggcaa
     2281 ctgaaaaata tcaccacccg ccagggatat gccgaaagtc tgccatttgc cgataacgca
     2341 tttgatattg ttatcagccg ttattctgcc catcactggc atgatgttgg tgcagcactg
     2401 cgtgaagtga accggatatt gaaacctggc ggtaggatga ttgtgatgga cgtaatgtct
     2461 ccgggtcatc cagtgcgcga catctggtta cagacggtag aagcattacg cgatacctct
     2521 cacgtacgaa actacgccag cggtgagtgg ttgaggttaa tcaatgaagc caatctgata
     2581 gttgataatt taattacaga taagttaccg ctggaatttt gttcatgggt cgcgagaatg
     2641 cgtacgccag aagcgttagt agacgctatt cgcatttacc aacagagcgc atcgacagag
     2701 gtgagaacgt attttgcctt gcagaatgac ggctctttca ccagtgatat catcatggta
     2761 gaagcacata aagcggcata aataaaaaag gcaccggggg aatcggtacc tttttattat
     2821 ctggtttgtc aggaatctgg catgttgttg tttttcacaa acaacgtcag cttgtcgcct
     2881 ggttgcagat tcgcagtgtc gctgttccag cgcatcacat ctttgatgtt cacgccgtgg
     2941 cgtttagcaa tgcttgaaag cgaatcgcct ttgcgcacac gatacgtaat gctatcgctg
     3001 ttgtttgcca gtcgctgtgc gctactgcct gcaccaatcg tcaaactttg gcctggcttc
     3061 agcttagatc cgcgcagttt gttccactgc tgcaaatctt tggtgcttac gccgagacgt
     3121 gaagcgatgc ttgaaagcgt gtcgccagag cgtacggtgt aaacacggct gttaagcggc
     3181 gtattgtcgg caaccagcgt cgactgtaca gcagcaattt cgcctgaagc gagagattca
     3241 cgcagttgat ctgcatgctt ctttggcacc atcacgtact gcggcccact tgcgcccagc
     3301 gtggagcctt tcacgccagc gttgaatgtc ttcagcttgc tgacggaaat ccccgccata
     3361 tctgcaacct tcgccatttc aaccgggcta ctcaggtgca cacgcgccag agcacggctt
     3421 tcatcggtcg ttggcagacg tacgccataa cgcttgctgt ttttgagaat atcactcaat
     3481 gccagcattt taggcacgta ctgcttcgtt tcctgcggca acggtaacga ccagaagtcc
     3541 gtggatttcc cacgcgcttt gttcgtttta attgccttca tgacccgacc ttcgccgctg
     3601 ttataagccg ctacggtcag aagccagtcg ccgtcaaaca ttttgttcag acgctgcatc
     3661 atattcagcg cggcagttgt tgaagcaaca acatcgcgac gcgcgtcata attgcgggtc
     3721 tgtttcaaac cataattgcg ccccgtgctc ggaatgatct gccagatgcc tgcggcattg
     3781 gcgccagacg ttgcgtgagg atcaaaagcg ctctccacta tgggtagtag taccagttcc
     3841 ataggcatgt tacgtttttt aacttgccct gctatccagt acatatacgg ctctgcccgt
     3901 aaagttacat cgtggagata gctcttattg cgtaaatatt tctgtttctg ttcgcgaatc
     3961 cggtcatttt ccggaattcc catctttagc tcgtcgccaa tgaaagccca caagtcacca
     4021 tctggcgcga tagacgtccc atcgtccatc catcgtgcct gacttgtaaa ctttgctgct
     4081 tccccttgac cagctgcaga aaggctctgt gcgtgctgtt gaacgttgcc ggtactctgg
     4141 caacccacga gcaggacaga ggcgagtaat atcgcttttg ccttcatgtg tgtgtcaata
     4201 gttgcttaaa agacgaccga tcataacggc gaacggagcc gatgacaaga aagttttatc
     4261 agaacctatc tttctttgac cttaaccatg caaaacgctc ttcaggttgt tgcaataatg
     4321 tttcttcatt aattacatta attaaatcaa tatcttccgt tcttaaaaaa acattaattt
     4381 gccgctcatt tttcagaatt acgggtagtg ttatttgatt ttttgcccgt aactccttaa
     4441 ctttacgata ataatcattt atggacaaat cgtgcggaaa aatactcaaa gcaaacttca
     4501 tatttgataa cgtatattca tgagcacaac ataccaatgt atcgtcaggt aacgcactta
     4561 gctttttaag tgattgatac atttgtaaag ctgtcccttc aaacaaccga ccacacccac
     4621 cagaaaacag cgtgtcgccg caaaatagat aaggtttact gaagtaacag atatgtccta
     4681 aagtgtgacc cggtgtggta attacactaa attcatgccc taaaacgaag gcagtttcgc
     4741 catctttgac tacctgtgtt gttcccttat cttgtgtctc ttgtggacca tacaccacaa
     4801 tttgcggaaa cttttccacc agttctttta cgccgccaac gtgatcgtgg tgatggtggg
     4861 ttagaaatat ggcctccggt tgccagttat tggcggtaat ggcgtttaat actggctctg
     4921 cgtctccggg atcgacaatc aggcagcgac ctgcctcatc attcaaaacc cagatgtaat
     4981 tgtcatcaaa ggcgggaata ctgttaagat tcatagatta cctctcagtg taaaacggaa
     5041 ggttgtgatg aaaccggcaa gagtccctca aactgtcgtg gctcctgatt gctggggcga
     5101 tttgcccagg ggagagcttt atcgcaaggc gctggagcgc cagctcaacc cgtggttcac
     5161 taaaatgtat ggttttcatc tgcttaagat tggcaattta agcgcagaaa tcaattgcga
     5221 agcgtgcgcg gtttctcatc aagtgaatgt ttctgcgcaa ggaatgcccg tccaggtgca
     5281 ggcggatcca cttcatcttc cttttgccga taaatccgtt gatatttgtc tactggcaca
     5341 taccttgccg tggtgcaccg atccgcatcg tttattacgt gaagccgatc gggtactgat
     5401 tgatgatggc tggctggtga ttagtggctt caaccccatc agtttaatgg gattacgcaa
     5461 acttgtgccg gtattgcgca aaacctcgcc ctataatagc cggatgttta ctctgatgcg
     5521 gcaactggac tggctctctt tgttgaattt tgaagtgctg cacgccagcc gtttccacgt
     5581 tctcccgtgg aacaaacacg gaggaaaact attgaatgcg catattcctg cgcttggttg
     5641 cttacaactt attgttgccc ggaaacggac tattccttta acgctaaatc cgatgaaaca
     5701 gagtaaaaac aagccacgaa ttcgccaggc ggttggagcc acccggcaat gtcgtaaacc
     5761 acaggcttaa acttcgactt ggtagcccgt atcttccagt gtgggattca tcgccgcggc
     5821 acgagccagt tcatcacagc gttcgttttc cgggtgtccg gcatggcctt taacccattc
     5881 ccatttgatt tgatgctgcc ccaatgcagc atcaagacgt tgccagagat cgacattttt
     5941 tactggtttt ttgtctgctg ttttccagcc acgttttttc cagttatgga tccactgggt
     6001 gataccctgg cggacatact ggctgtcggt actcaaaatg acttcgcaat gttcttttaa
     6061 cgcctccagc gcgacaatag cggccatcaa ctccatacgg ttgttggtgg tgcgggtgta
     6121 gcctgcgcta aaggttttct cgcgtccgcg atagcgtaaa atagcgccgt aacccccagg
     6181 tccaggatta cccagacacg aaccatcggt gaaaatttct acctgtttaa gcatctctgg
     6241 tagacttcct gtaattgaat cgaactgtaa aacgacaagt ctgacataaa tgaccgctat
     6301 gagcactgca attacacgcc agatcgttct cgataccgaa accaccggta tgaaccagat
     6361 tggtgcacac tatgaaggcc acaagatcat tgagattggt gctgttgaag tagtgaaccg
     6421 tcgcctgacg ggcaataact tccatgttta tctcaaaccc gatcggctgg tggatccgga
     6481 agcctttggc gtacatggta ttgctgatga atttttgctc gataagccca cgtttgccga
     6541 agtagccgat gagtttatgg actatatccg cggcgcggag ttggtgatcc ataacgcagc
     6601 gttcgatata ggctttatgg actacgagtt ttcgttgctt aagcgcgata ttccgaagac
     6661 caataccttc tgtaaggtca ccgatagcct tgcggtggcg aggaaaatgt ttcccggtaa
     6721 acgcaacagc ctcgatgcgc tatgtgcccg ctacgaaata gataacagta aacgaacgct
     6781 gcacggggca ttactcgatg cccagatcct tgcggaagtt tatctggcga tgaccggtgg
     6841 tcaaacgtcg atggcttttg cgatggaagg agagacacag cagcaacaag gtgaagcaac
     6901 aattcagcgc attgtacgtc aggcaagtaa gttacgcgtt gtttttgcga cagatgaaga
     6961 gctggcagca catgaagccc gtctcgatct ggtgcagaag aaaggcggaa gctgcctctg
     7021 gcgggcataa atacatgtaa aaggcgctaa aaatagcgac ttgggcgatt tttgcagcaa
     7081 acgattcaaa agatgagaaa aatcgttgac gaaggtcgag gcaatccgta atattcgcct
     7141 cgttcccaac ggaacacaac gcggagcggt agttcagtcg gttagaatac ctgcctgtca
     7201 cgcagggggg cgcgggttcg agtcccgtcc gttccgccaa tattcagaaa gcccgaatca
     7261 gtaatgattc gggctttcgt cgttttggtg aagtgagaat ttttcactcc gaagtaaaac
     7321 ggatatgatt taaaaaatgg ccagacatta taaattctgg ccacttctgt tactccagcg
     7381 catcccattt ctccagcagt tctactggaa tttcatttat tacctccgag acgcgttttc
     7441 cgaccaggcg ttctgcctgg cgcagcaaag gaatggtcgg gctgctcggt tcgctgtgct
     7501 caaaccagcg acggatgaca cgcaggcgtt ccagcgcgtc gtttcggtcg cggatctgcc
     7561 ggggttctgc gccggtcatc ggtgatattt cctccgcgat atgcaccggt agcgcaatct
     7621 gctctgcctg catctgttca gtatgaacta tggtggcgtt ttccggctgc tcagcctgtg
     7681 gttgtggcag gatctccggc gtggtgctct gcactgctcc cggtaacagg gccagcagtt
     7741 gcgtcagacg ggagaagtcc ggtgcctggt cattcagcgt ttcacgcgca tggcgttgca
     7801 gccgctcagt ggtctctgct gcctggcgaa aggcatccag cggcagtgtg ccgcgcgctt
     7861 ccaggtctgc cagttgctgg cgtaccgatt ctggtgccag cgcatccgcc gggcgtaatg
     7921 cagatagcga acgctccaca tcccgcacct gaaggcgcat ggcagcatta tttgacagtg
     7981 taatgccacg aatatccgcc attacacctt cgtggtccag caacgccgcc agcgcgttac
     8041 tgcgtgccac cgcagcatct tcggcggtga tatcttcagt cgcgagcagt tgaggatgga
     8101 tggcgtccgg gtacgtggag cacaacgttt ccagcagcgt gagcgcctcc gacagtcccc
     8161 tggcaccggc ctgctggata cggcagcgca gcaggacaac cagaatgcgg atatctttgc
     8221 tgcgcatcag cagacgatgg gcgtcccgct caagctccgc ccagttaatg ttttccggcg
     8281 tactgacaaa atcgccatat tgcgcttctt cacgcggtgc ggcgcgggtg aacagcagca
     8341 ggtattccgg atcgtactcc agatccgggc cgcaggggca ttccgccgaa attgggtcag
     8401 acaggaatat ttccatttaa ttatcctcag tgcgccaggt agtgttctgg ttcgaaaatc
     8461 atgcccgtca ccgggttgtc gtgcatggct ttacccgccc atgcagtcca acccagttgg
     8521 tacggaccac cggtaacagc cggaggggcg gcgtggggct ttagctgtag ctcaatttcc
     8581 cagcagtatt cataaccact aaaggcgcgg accagttctg tcaggacggg caggttattg
     8641 ctgccaggca gaaaacgtcg gtaatcctcc agcgtcagcg ggccgataac cagccggaac
     8701 ttatactgca tgtccggcac ggcctggccg atgagtgcac cattgccaat aacggaagag
     8761 ggggcaggca tccccagccg ggtgatctca tcgtccgcca cggctatcca gtgcaggaca
     8821 tactcctgta tccggaaagg tacaccgaaa tagtgcgcca gcgtggtggc cagaccgtcc
     8881 ggattgcggg actcacgcac cagatgggcc gatgccgcca gacgagcgtg atctggcaac
     8941 gggctgtcag cgctctcccg caaatcctgc ccgctgaggc tggcgacata aaaggcaaag
     9001 cggtcatgtt gcggtttatc cagaccgccc ccagcggact gggcgctgcg ccatgcctgg
     9061 taaaactgcg tcagccagcg gtgatgaaaa agatcagaaa aacgggtcag cgtcggatcg
     9121 cgtcggcttt cagtccggtt acgcgccagt tcggtgtagt gcagcggcag tggaccgttc
     9181 ggcccccaca gcccgaggct gtacagactg atgtgcaggc gcccttcctg ccagcccaca
     9241 ctggcgattt ctcgtggcgc aaaggtcagc gcgggcgttt gtccgaggcg aaatttttcc
     9301 atccgtggtt gctaggtgtt gcccgccgga acggtacaca actgcacatc cacgcggcgc
     9361 atcaggttca gaaaaccgta atgccagggg ggttcctgcg cctgagtgaa atcgttgctc
     9421 atacgcttcc ccgctgtccc gccctgacag gccaggtcat aattttgccg cgctgcatgc
     9481 tgtgcagcgt catctgcgag aaggtattaa tggaaacatg gcgggcgata tagtgttcca
     9541 gcaccaggcc gaacagatac ggactgatgc cggaaaaacc ttcttcatcc accgtcagtt
     9601 cacaactgac gccacgccca tagaccagta gtccggaacc gggcagacgg cgggtgaccg
     9661 gggtcgtttt acaaccaatc aggctgcgca cctggcgcga ttgcggactg tcatgagcag
     9721 gaataaacag gttcagcaga tctcgcaacg cctggccgcc ggtacggtga tccagatcgg
     9781 ccagcggcag ataattaaaa gataactggc ggatcaaccg ccatgccatc tcacgttcag
     9841 ccagcggggg ctgcggcgag cgtggcgggc ggataagccc tacgcccgcc accgggatgg
     9901 ctgcatccac ggttagatcg tcccggccat tgcgggcaat aagacagggc agatcgcggt
     9961 tagtgaccat ggccgtgatg gtgatgtggc gcagattttc cgggtacggc gcttcatact
    10021 ggtcaaccag cgacaggaac acttccgagc ccgtataggg ggtacgggtg ccgtaacggc
    10081 gggcgttttc tgataagcgg cggggctccc ggcgcaggga aaaataacgt ccgtgattgc
    10141 cttcatcatt attgcgggtg tggtagagcg ggcgaaaggc catcttccgt gtggtgtcgg
    10201 tttccagccc ttccacttcc tggaccgaga acacctcata atccagcgga tgggttctgt
    10261 ccaccaccag atgctgttcc gtcgtgctgt gcgtgacatc gatccgggcg gtgacgcggg
    10321 gaaacaggtt gataaccggg gtacagaaca ggctgaactg tgccgcattt gtctggtgga
    10381 tcagtcagtc cggcggcagg cggttcagca ggataacaat ctccgccacg ccaccgtcaa
    10441 ttttctgtaa tccggcagac aggcctgtcg gggtgaaaaa atagaagcgt tccggacagg
    10501 caaaatattc atgcagcagg ttatgaccgt gaaacacgtt ccacgccagc ggcagcagcc
    10561 cctgatccgg ctccaggcct tcgtacataa cgggctgttg cagattcaca tccagcgcgc
    10621 cgtcaaaatg ccccggaatg ccagccaggg tcgcgatggc gctggtatgt agaagttcta
    10681 acaggtgtga ggccgtacgc tcctcgccgc agagataaaa cggcagcctg tccagtcctg
    10741 caagctgact gaacgttagt tcaccaaagg tgcgcagggt aatacgcagc gccccggcga
    10801 cgtgaatgtt ggccggcagg tagcggtgca gggcaggcat atccggtggc gcggcggtca
    10861 gtcgcgcctc ttcgatggcg agcggccaca gcgttacatc ctggctgctg cgaaactggc
    10921 aggcagtgct ttcgccttcc ggaatggcgg agaaaaaggc tgtgtcgcgc gggacggtaa
    10981 atcctttcgc cagatcgcct tcttccgtat cgggatgcaa ctgcgccacg ctcattgagg
    11041 gcgtgggtgt gacgtaattg gggctgacca cttccagcgg gcgctgggtg aaacggggga
    11101 attcagcgtc aatttttagc tgcgtgcggg cggtcaggaa gctgaaggcc tcgaccattc
    11161 gctctgcata cggatcggca atatccgttc cctgcatgcc cagccgggcg gcgactttgg
    11221 gatgacgggc ggcaaattcg gctcccgttt cccgcagata gctcagttcg cggttgtagt
    11281 atcccagtag ccgtggatcc atcggtctct ctctgaatta aaaaaacgta atgcgactct
    11341 gctccatatc cagcgcgcta cgtacccgga atgcagtcgg atacggctgc gtgaggatct
    11401 cgccgcgaat ttcaaactgt aatgtgttat ggccactctg gcggcgctca tcctgaagtg
    11461 ggatgatttg cagggtggac gctttcagcc ggggttcaaa ccgggtgatg gttcgcaaaa
    11521 tggccctgcg gatatcgtcc cacttgtgct catacagaaa gctgcccgcc agtggcggca
    11581 ggccgtagtt aagtacggac gctgccgcgt acggataacg tgcagcatca atatcatctt
    11641 catggctgac ggtgttgagc agaaatgact aatcccggcg gatgatctcc ttaatctgaa
    11701 ccgggctgac gctgataccg tggtcgcgtt tctggtttgg ggcgtcgtca cacaggcggt
    11761 caaacagcgt gggcagcagg tgattgcctg gacgctggcg ggaagcgctc acgcgtcgct
    11821 tccttcagcg gtgttaaagg tacaggtgga caaatccagc aggctgatat cgccgtgacc
    11881 ggtgagccac accctctgac ccagcgcctg taccagtgtc tctcccggac cgtcctgcca
    11941 gaccgtttcc cggcacagac gcagggagtc ggcagccttt tcagagccgc tgtagcgggg
    12001 aaacagccag gcactgtgtg tatcgccatt gttcagggtg acgttgacgg gtttccagat
    12061 caaatcgatc agatgcgcag gccgcggcga tttcagcgaa cagatttgtg aaaacggcag
    12121 ccagatatat gctccgcccg cgatcagttc gagtaccgga ccggtacggg agtcgctgtc
    12181 gctaatccag tcaaatgcgc cgccattcca ctgcccggaa gtgtctgtta tggcctcaag
    12241 cgcgcggctg cggtgagcat cagcttcctg cgcttcacca cgggcgttac aggcgagggt
    12301 ggtcagcaga tcctccatcc aggcgggcgg cgggagaata acaccgggcc gctgttcgcc
    12361 ctgaaagcag gcgtggcggt atatctcaca gcggatcagc tcgccaaata cctgcgcttc
    12421 gcgagtataa tttgcgtcca tccgggcgca tagctggatc tgttgcagtg cccgcgacca
    12481 gtcgcctgcc acgcacaata actgaaacag gctgtggcgg cgggaggcct gcgctgggtg
    12541 catcctgacc tgttgctctg ccatctgaat gccctccacc acggaataat cccgcagcag
    12601 cgccgacaag gtaatcggta gctcgttatt gtttgtcatg atatatctct ccatgttgtg
    12661 ccgggtttat ccggtaatgg ttgtcaatgc caatgatgcg atgctctttc cgtgtcagat
    12721 ctggcagtac ggtgttatgc gctgtttccg gggcgtattc cggggaaagt aaatgcagga
    12781 tatcaggggg agtcttttcg acaggccaga tagtttcatc ttcgccagtg gcatccagta
    12841 cattgagaat agcgtcaatg cttaacccac cagtgaccat atcctgcaat gtctcggctt
    12901 cggtgaccag gtcacgcaat gaatccagat cctgcgagga gggctcttcg ttaaaaggaa
    12961 aaggatcagc atggcgatta agatcttgtt cgggagataa caacgcctcc ggtattcctg
    13021 ataaagtcgg gtcagggcat ttcctttttc cgcaccttct ccggtggagg aggctccatg
    13081 aacaggaatg atatcgaatg gattttgtga tgtcagatgc tgctgcctga accaatccag
    13141 atccagatat tcggttattg cttccgacgt gatggcagtc tgcgatatcg tttttactgt
    13201 gctcaggtcc ggagatgatg gattgccatg tacaaggcac tatgaagata acccccattc
    13261 tatcaggtcg ccttcattca gacgcatacg gtgatgaggt tccacaatct ggtcgtttac
    13321 cgtacagcaa tattcggtag actgattgat aatccaccat gcttcttcgt ggcgggtcag
    13381 agtgagggca atctttacat catcattcac cttaaaaaca ggaatccagg gagaggtaac
    13441 ggtgaagata atttgtgttc cttcagtcgc gtaaattgtg ttatcggcga tcccgtgcgc
    13501 ctgtattttt tggagtcccc acgtaatatt tggtttc
//
LOCUS       DAJTYG010000096        13492 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTYG010000096 DAJTYG010000000
VERSION     DAJTYG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.096"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..548
                     /locus_tag="OO568_003125"
     CDS             <1..548
                     /locus_tag="OO568_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6642384.1"
                     /translation="RIKAVVSITGVNIGRLFREGFSNYDPIGVLNAMASQRTKEARGG
                     ELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGAAKMLFSHAQKTLAWD
                     AFAFTEVLLTQPVMVVVGEKVGAFGVYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYD
                     KPEAVQEALAKVIPFFNTHLG"
     gene            566..1204
                     /locus_tag="OO568_003126"
                     /pseudo
     CDS             566..1204
                     /locus_tag="OO568_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1495..2223
                     /gene="mpaA"
                     /locus_tag="OO568_003127"
     CDS             1495..2223
                     /gene="mpaA"
                     /locus_tag="OO568_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6642385.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2198..3163)
                     /gene="ycjG"
                     /locus_tag="OO568_003128"
     CDS             complement(2198..3163)
                     /gene="ycjG"
                     /locus_tag="OO568_003128"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6642386.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3282..3788
                     /gene="tpx"
                     /locus_tag="OO568_003129"
     CDS             3282..3788
                     /gene="tpx"
                     /locus_tag="OO568_003129"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6642387.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGLLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3832..5373)
                     /gene="tyrR"
                     /locus_tag="OO568_003130"
     CDS             complement(3832..5373)
                     /gene="tyrR"
                     /locus_tag="OO568_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6642388.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5521..6582)
                     /locus_tag="OO568_003131"
     CDS             complement(5521..6582)
                     /locus_tag="OO568_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6642389.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6579..7976)
                     /locus_tag="OO568_003132"
     CDS             complement(6579..7976)
                     /locus_tag="OO568_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6642390.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQTTTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8131..9129
                     /locus_tag="OO568_003133"
     CDS             8131..9129
                     /locus_tag="OO568_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6642391.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKKSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9240..10145)
                     /gene="ompG"
                     /locus_tag="OO568_003134"
     CDS             complement(9240..10145)
                     /gene="ompG"
                     /locus_tag="OO568_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCR6642392.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(10190..11272)
                     /locus_tag="OO568_003135"
     CDS             complement(10190..11272)
                     /locus_tag="OO568_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6642393.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTILEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(11286..11945)
                     /gene="pgmB"
                     /locus_tag="OO568_003136"
     CDS             complement(11286..11945)
                     /gene="pgmB"
                     /locus_tag="OO568_003136"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6642394.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11942..>13492)
                     /gene="ycjT"
                     /locus_tag="OO568_003137"
     CDS             complement(11942..>13492)
                     /gene="ycjT"
                     /locus_tag="OO568_003137"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6642395.1"
                     /translation="ITVIADHDKPQPSGCLLEMCAQQSYDQHLAASTENWRQWWQKRR
                     ITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWDTEVFLLP
                     FHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETPEFAAINI
                     RTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAV
                     RVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDAFIHRAEM
                     FLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEVNEMQILK
                     QADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLTQSYQF
                     WREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPALPEQWQQ
                     LSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDFILPFNGT
                     ATTHQEDE"
BASE COUNT         3337 a         3655 c         3224 g         3276 t
ORIGIN      
        1 aacgaattaa agctgtagtg agtattacgg gtgttaatat cgggcgtctt tttcgggaag
       61 gtttcagcaa ttatgatccg attggcgtat tgaatgcaat ggcgtcacaa cgaacaaaag
      121 aggctcgtgg tggagaatta cagatcaacg aattacttcc cgccagcctg gatgcagcaa
      181 aagcccatgg actgaccgaa cgcgatgttt atgaagcaac tgactactat aaaacaccac
      241 gcgggcaaca accgggaggt gcggcaaaaa tgttgttctc acatgcacag aaaacgctgg
      301 cctgggatgc cttcgcattt actgaagtgt tactgaccca gccggtgatg gtagtagttg
      361 gtgaaaaagt cggtgccttc ggtgtttatc gtgatggact ggaagtctat ggccgggcga
      421 tggtttcgca ggatcgtcag ttagtttcgt tacccgattt ctcacattat gaactatatg
      481 acaaaccgga agcagttcag gaggcgctgg caaaagttat tccttttttc aacacccatc
      541 tgggataagt ttcatgagaa atattatgaa aaatgtactg attcttggtg ctggcggtca
      601 gatagcccgc catgtgatta atcaactggc agataaacaa actattaaac aaactttatt
      661 tgcccgtcag ccagcaaaaa tacacaaacc ctatccaact aatagccaga ttattatggg
      721 ggatgtactg aatcatgcag cactgaagca ggccatgcag ggacaggata ttgtgtatgc
      781 caaccttacg ggggaagatt tagatattca ggcaaattca gtgatcgctg cgatgaaagc
      841 ctgtgatgtt aaacgtctga tctttgttct ttccctcggt atttatgatg aagttccagg
      901 aaaatttgtc gaatggaata atgcagttat tggtgaaccc ctaaaaccat tccgcagggc
      961 tgcggatgca attgaggctt ctggactgga atatacaatt cttcgccctg cctagctgac
     1021 tgatgaagat attattgatt atgagcttac ctctcgcaat gaaccattca aaggtacgat
     1081 cgtatcacgt aagagtgtcg cagcattaat tacagatatc atcgataaac ctgagaaaca
     1141 cattggtgag aatataggca tcaatcaacc cgggactgat ggcgataagc cattcttcat
     1201 gtaaatcaat tatatagata ccatcgtact gtactatgtg cagtacgatt tattaaactt
     1261 attaaggtga actgatcaat cagatgcgat taacatgtgt agaaccagta agaataaaat
     1321 acgccatgta tataacatta cgacaattct aatatctgca catcaatgaa aatgcaaaca
     1381 acttccggca aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata
     1441 atccgcaaaa tttgcacaac actgcctgta atacttcctg aaccagagac gatcatgacc
     1501 gtaacccgcc cacgcgccga acgcggcgca tttccgcccg gaacagaaca ttacggacgt
     1561 tcattattgg gcgcgccgtt aatctggttt cctgcccctg cagccagccg cgaaagtggt
     1621 ttgattctgg ctggcactca cggtgatgaa aactcttcgg tcgtcacgct ctcctgtgct
     1681 ctgcggactt tgacaccttc tttgcgccgc catcatgtgg tgctgtgtgt gaatcctgac
     1741 ggctgccagc tggggttacg ggccaatgct aatggtgtgg atttaaacag aaactttccg
     1801 gcggcgaact ggaaggaagg tgaaacggtt tatcgctgga acagcgccgc tgaagaacgt
     1861 gatgtcgtat tactgacagg tgacaaacct ggctccgaac ctgaaaccca ggcgctgtgc
     1921 cagcttatac accgcattca acctgcctgg gtggtctcat tccacgatcc actggcttgt
     1981 attgaagatc ccagacacag cgaattaggc gaatggctgg cccagtcgtt tgaattgcct
     2041 ctggtaacca gcgtcggtta tgaaacgcct ggctctttcg gcagttggtg tgcagatctc
     2101 aacttgcatt gcatcaccgc agagtttccg cccatctcct ccgacgaagc cagcgaaaaa
     2161 tatctctttg cgatggcaaa cttgctgcgc tggcatccta aagatgcaat tcgcccgtcg
     2221 tgaactgaag cgccggttcc acatctaccg ccagccaggt cggtccgtca agatcggcga
     2281 aactgacctg cggcaccagc ggtaaagcgg cgctaatggc acgagaggta cacaacatgc
     2341 agcccagcat cagactgaaa ccttgtgcac gcgcttcagt cgccagcgcc agcgcttccg
     2401 tcagaccacc ggttttatcg agcttaatgt taaccatctc atagcgccct ttcagcgcct
     2461 tcagattgct acgagtatga caactttcat cagcacaaat cggcaacgga tgaataaaat
     2521 tctccagcgc cgcatcgtcc tgcgcaggaa gcggttgttc aagcatcgca acgcctaaat
     2581 ccgccaatag ctggcaacgc gccgccaacc cttctgcacg ccaggattca tttgcatcaa
     2641 cgatcagcgt ggcatcgggc acagcggtgc gaattgccac catccgctca ctgataagat
     2701 ggttatccag cttcactttc agtaatttcg cgcctgcctg ccagagtgtt gatgcactat
     2761 tggccatctg atcaggcgta ccgataacaa ccgtctgtgc agtgataact gtctcgggaa
     2821 gcgttatgcc gatcaaatca gccagcgatt gctgctgttt tcgcgccgcg agatcccaca
     2881 atgcacaatc cagcgcatta cgtgctgcgc cggcagggag aattttttgc aactcctccc
     2941 gtgtcagccc tttctctaat tgcggcacga cgctcataat ttgcgccatt accgaggcat
     3001 cactttctcc ataacgcgga tacggcgtgc attcgccggt gcctttaata ccctcttctt
     3061 ccagttcaac caccaccacg cgcgcttcac tgcggcttcc ccgggcaatc acaaacgggg
     3121 tatgtaaggg ccaggcttcc tcaaatacct taacgattct catctttact ccttgttgtc
     3181 gcggtggtct gcggattaag cgcaaatagc gtttgctgtg ttattgacag ttagcataaa
     3241 ctaggtgtga cgttaactat atgtaaacag gaaagataat catgtcacaa accgttcatt
     3301 tccagggcaa cccggttaca gtcgccaatt ccatcccgca ggcgggtagc aaagcgcaga
     3361 cttttactct cgtggcaaaa gatctgtctg acgtcaccct cggtcagttt gcgggtaaac
     3421 gcaaagtgct gaacattttc ccgagtattg ataccggtgt ttgcgccgca tcagtacgta
     3481 agtttaacca actggcaact gagatcgaca acaccgttgt gctgtgtatc tctgccgatc
     3541 tgccgttcgc ccagtctcgt ttctgcggcg cagaaggtct gaacaacgtt atcaccctct
     3601 ccactttccg taacgctgaa tttctgcaag cctacggtgt ggcaattgct gatggcctac
     3661 tgaaaggtct ggcagcgcgt gccgttgtgg ttattgacga aaatgacaat gtgattttca
     3721 gccagctggt ggatgaaatc accaccgagc cggattacga agcagctctg gctgtactga
     3781 aagcataagt taatatgcct gatggtgttg caccatcagg catattcgcg cttactcttc
     3841 gttcttcttc tgactcagac catattcccg caacttattg gcaatcgcgg tatgtgaaac
     3901 gccgagacgt tttgccagtt tgcgcgtgct gggataattg cgataaagct gggttaatac
     3961 cgagcgttca aaacggctgg tgatttcgtc cagcgaacct tccatcgcat cttcgcccac
     4021 ggctaccgtt gctgcgtcat aatccggcaa caaaatatcc tgtggacgca gctcataacc
     4081 gtccagttgt gtcagtgcgc gatagatagc gttctttaac tgccgcacat ttcccggcca
     4141 cgcataacgt gtaagtacag tattcaggtc agcggccagt ttcggacgcg gcacgccctg
     4201 ctcgtcggca aagcgggcga cgaacagctc agttaacggc ataatgtcct gcggacagtc
     4261 acgtagcggc ggcagattga gcgtcaacac gttcagacga taatagagat cttcacggaa
     4321 cacgcctttt tgcaccagtt cgaccagatt cttctgcgta gcgcaaatca cccgcacatc
     4381 gacatgcacc tcatggtctt cgccaacccg acggaaagtg ccatcattaa gaaaacgcag
     4441 taatttcgcc tgcatccgtg gtgacatttc ccctatttca tccaacagca ccgaaccacc
     4501 gttcgcctgc tcaaagaacc ctttcttccc ttccggagca tgaccaaaca gttcactctc
     4561 gaccgcatct tctggtatag acgcgcaatt cagcgccagg taaggtttgc ccgctctggg
     4621 gcttgcctga tggcaggcgt aggcaaaaag atctttacct gtacctgtgt cacccgtaat
     4681 cagcagcggc gcgcttagca tcgccagttt ctgcgcctgt tcgacaacgt gcttcatttt
     4741 cgggctgacg gcgacaattt ggctgaaggc gctgacgtcc tgggcggcga cattttgcaa
     4801 ctggcggccc atacgaatcg ttgatcgcaa catcaccacc gcaccggtca ggacgtgttg
     4861 atcattttca tcctgaagat aaacaggcgt aatctccatc aggaaattct gcccattaat
     4921 aacgacatgc tcgttatgcg aatcttgcgg ttcgctttcc agccaacgta aaaaattaaa
     4981 gccgttaatc aattgtgcgg cggtatggtt gcgcaggcga tccaattttt gcccaaaaag
     5041 ctgacagctc gccgggttcg ccatatccac tttgcttttc atatcgacag agagcacagg
     5101 ttcaggcaac gcctccagta acgcgctcaa cgccagatgc tcacgttcgg aaggcatcca
     5161 cgggacagta cgcacatcgg taacacctgc aatacggcgt atttcggcca tcaaactgct
     5221 gaaactctca aactccagtt cagcaaaatt gaggtagatt cgcccaatgg gatcaatctc
     5281 aataccgcgt aaatcaatgc ctcttagcac gagtagatcg agtaattcgc gggtcagacc
     5341 gagtcggtct tcacaaaaga cttccagacg catgggaacc ttcacctgaa aaaagaacaa
     5401 ttaatatgat gatatgacac tatttggata gcaggaaggt ttctgtcaac aatcattgac
     5461 acaaagacgg aaaaacgggc acttcggcgt aaagatatcc ggtcagcacc cgatattgca
     5521 ttatttttcg ctgggcgttt tgcctttttg cagagtttct ttcacctgac cgataagctg
     5581 acgacggaaa tccccaaggc gaggtttgtc atcgtcaatc cacggcagcg ggcggcaaag
     5641 ctccatagct ttaatcccga gtcgtgccgt cagaagtcca gcaccaattc cctgagctgc
     5701 gcgggtagac aaacgagcag cgagatcttg cgacatccag tccatcccca cttcgcgcac
     5761 cagttcgctg gctccggcaa aagcgatatt cagcaatacc agcttaaaca gacgcaaacg
     5821 gctgtaatac cccagttcaa tgccatacag cgtggcaatg cgattaatta aacgcagatt
     5881 gcgccaggcg ataaacgcca tatcgaccaa cgccagcggg ctgaccgcaa tcatcaacgt
     5941 tgattccgcc gccgaacggc tgatttcgcg ccgcgcctgg gcatctaaaa ctggctggac
     6001 caaatgcgca tacaggctga ccacttcacg atcgttctgc gtttcatgga ttgaggcata
     6061 ccagcgttgc agcgccggat gcgactgatc aatacccgcc tgctgcgcca gtttttcgca
     6121 aaatgcgcga cctttgcccg tgccgtggct atgcaataaa tcacgcgctt cgtcgcgttc
     6181 atgggcgcgc tgtcgcaagc gccataagcg ccgccactct gttaccacag aacctacgcc
     6241 agcgccgata atcaatgccc cagcggcaca tccacccagc gctacccagt cctgggtttg
     6301 ccaggcattc attgtccact gtataccctg cccgacaacg cttgcgccaa acagagccag
     6361 cccgcccatc accattttgc gccacaggct gcgtttcgga cgtaacgctg cgtccattac
     6421 cgcttcgact tgcccctctt cttcctgcgc ttcgtcgagc gtggccgggg caaaattttg
     6481 cgcctgattt tcgtcaaagg tctgctgcgc cctgaatttt ggattctgtt cgacctccag
     6541 cggaccgtcg aaatcaatac gtggttttaa cggttcggtc atcgcaattt atctcctatt
     6601 aaaaattcca gcgcggcatc aagacgaata tgcggcagcg gtttgtcgac atccatcacc
     6661 tgcgggcgaa acgcttcaaa ctggaaccct tgcttgtccc agaacgcctg accgggcaaa
     6721 cgcgcaggaa cttcaccggg gtaaacggtg agaggcgcac catcgctgag ccggttaccg
     6781 cgcaatgccg gaattttctc gccgttgacg tcaataatgc cgctggtggt cgtctgcacc
     6841 gaggccagcc ccaggcaatc catactaatc ctttcaaacg ccgcattttg ccaggcatcc
     6901 tgaatcagtt gttgcagtaa tgaaaccata ttggcgtgct gatcgaccgt tacatggtcc
     6961 gctttcgtcg cggcaaacag cagtttgtcg ataaccggcg agaataagcg gcgaaacagc
     7021 gtgcgctgcc cgtagtggaa actttgcatc agctgcgtca gtgccagacg catatcatta
     7081 aatgcctgtg gcccactgtt gagaggttgc aggcaatcca ccagcacaat ctggcggtca
     7141 aagcgcagaa aatgattctt atagaacccc ttcaccactt tctcgcagta ataattaaac
     7201 cgctcgcgca gcattccggc attggtgtgc ttatcggcct gcgccagttt ggactcgccc
     7261 caggcatcga catccggcca cgggaagaat tgcagcgcgg gcgcacctgc catatctccc
     7321 ggcaagacaa agcgcccagg ctgaataaag tgcagcccct gctgtttaca gtggtggaga
     7381 taatcggtcc acacggcggc aatgtctgcc agccggtttt cgtcggcagg cgctagcggg
     7441 tcaagtcctt cgcacatcat tcgccatttc gccgaccatt cgccgcgctg accattgagt
     7501 aagcccgtca tctggcgcga ccagcttaag taatcctgcg ccagcatcgg caggtcgagc
     7561 aaccattcgc cgggataatc cacaatttcc agatacagcg tggaggtatc cttaaagtgg
     7621 cgcagcagcg aatcgttcga tttaaaacgt aacgccaggc gaatttcact gacgccgcgc
     7681 gtaggcgtcg gccaggcggg aggatcacca tataactgcg ccagtccttc atcataggtg
     7741 aagcgcggaa tgccaaagtc acgctgggga atgcgtttta cgcccagcaa gcgctcttca
     7801 cgcaccgcgc ttaacagcgg caaacgtgct ccggcatgaa tattgagcaa ctgattaacc
     7861 atcgcggtga taaacgctgt tttgccgctg cggctaagtc cggttacagc gaggcgcaga
     7921 tgtctgtcga caccccgatt caccagcgca ttgagttcat ttttaagtcg cttcattgct
     7981 gtcctgtgct gctctgaaga ccagaaaaaa atcattcgta aggatatgaa tgtgggggcc
     8041 aagccattga ttatcaactg cccgtgttgt catcgacggg cgaactccat aagatgtgca
     8101 gcattcacgt tcggacggag ttcctgacct atgtccccta ctatttatga tattgccagg
     8161 gttgcaggcg tatcaaaatc caccgtatca cgcgtgctga ataagcaaac taatatctcc
     8221 ccggaagcgc gcgaaaaagt gttacgggcc attgaagaat tacagtatca gccaaacaag
     8281 ctggcccgcg cgctgacctc ttcgggtttt gatgccatta tggtgatttc tacccgttcg
     8341 accaaaacta cggcgggtaa tccgtttttc tctgaagttt tgcatgccat caccgccaaa
     8401 gcggaagaag aaggtttcga cgtgatatta caaacgtcgc acaatccggc agaagatctg
     8461 caaaaatgcg aaagcaaaat taaacagaaa atgattaaag gcattattat gctcagttcg
     8521 ccagcggatg agtcattttt tgcccaactc gataaatacg atattccggt ggtggtaatt
     8581 ggcaaagttg aaggtcaata tgcccatgtt tattctgtcg ataccgataa ttttggtgac
     8641 agcattgcgt tgaccgatgc attaattgaa agtgggcatc aaaatattgc ctgcctgcat
     8701 gctccgcttg atgtccatgt ttcggttgat cgcgttaatg gctataagaa aagtctgtcc
     8761 gcgcataata ttgcagtgcg tgataaatgg attgttgacg gcggttatac ccatgaaaca
     8821 gcactgcaag cagcacggca attattaagt cagtcaccgt tgcctgaggc agtgtttgcc
     8881 actgacagcc tgaaattaat gagcatttat cgtgcggcgg cagagaaaaa tattgctatt
     8941 ccgcagcagt tagcggtggt gggttatagt aatgaaacgc tgtcgtttat tttaacgcct
     9001 gcaccaggcg gcatcgatgt tccgacgcag gagttaggtc aacaaagctg cgagttatta
     9061 ttccgcttaa tttcgggaaa accgtcacca cagaatatta ccgttgccac gcatatgacg
     9121 ctgaaataaa taccagctac cttgcctgaa tgccggatgc gatgctggcg catcttatcc
     9181 ggcctacatt tatagatcgg ataaagtact cgcgccgcat ccggcaattt agcccattat
     9241 cagaacgagt aatttacgcc gacacctgca taatggaatt tatcgctgtc gccttcgtcg
     9301 tgatcctgcc actcaaacgc gtattccagc gaaacggaaa gtccgttctg gaaatcataa
     9361 ccgtaaaata aacccacacg gttaaaatca tggccttcac gttcaatatc atcctgccag
     9421 tcccagttac tccagcgatc cagcccaatg cgcgtatacg gcgtcaccga gtggttgccg
     9481 agcgtcagcg gcaaataggc gcgaatttct tgtgtggaaa actcaccgtt attgcggctg
     9541 tcgtccatat tgaagccgcg ctcgagataa tagttcactc gcaaagcaac cgtttcgttg
     9601 aaggtatatt gcagacctgt ttccgtttcg acacgggtat cagcgtaacc ggtcgtgttc
     9661 aaatcgttgg caaatttata catcgacaac caaccgttga aacgtaaatc atcagtcagt
     9721 ttcacatccc agtctggcgc gattttccag cgctgcatat tcgccgtgtc ttttcccggt
     9781 tcatcaacgt agtgataacc ataattacgg aaaccgccgg tcaggccgaa actgaaatca
     9841 tcgctttcga ggaactgata atgcacctcc agctccgggc gatcaaacca cgttccacgt
     9901 ttacccgcgc tataatctac cggcccttcc tgataatagg ccagagcaat tctccacggc
     9961 ccgttggcgg cattaaaata gactgaaggc tccgccagcc catccatatc ttcgccataa
    10021 ccctcgacgt tttctatttc gtacatcgca ccgatattaa agtgccagtc gttcttttcc
    10081 tcggcctgtg cgcaggccat tcccgcacac atcaccagtg cggtacaagg taataacttt
    10141 ttcattattg ttccttgtat tatcctaggg gaatgccttc cccctgtatt tagcgaattg
    10201 ctatttccgt ttctgcatca aagaaatgac atttcgtcat atcaaaatga atagtgatat
    10261 tttctcctgc atgataatca tttaacgcac cagcacggac cactaactcg tgccccccaa
    10321 ccgtggtgta gagcataaat tccgcaccgg ttaattctgc cacgctaatt ttggcggaaa
    10381 tgttattttc ctcttgcgcg tccggatgaa tatcttccgg gcgtattccc atcacgatgg
    10441 gcttatgcaa actttcctgt gttttcagaa ccgctaattt ctcttcgaga atggttaatt
    10501 taagcgtttc cgtaacgaat ttatcgccat cgatcgtgcc gcgaataaaa ttcatcgctg
    10561 gtgatccaat aaatccggca acaaacatat tcgcgggttg gttataaacg gttttcggcg
    10621 cacctacttg ctgaacaatc ccgtctttca taatcacaat ccgcgtcgcc atggtcatcg
    10681 cttcggtctg atcgtgggtc acgtagatca tggtggtgtt cagtttctga tgcagcttgc
    10741 tgatctctgc acgcatttgt acgcgcagct tggcatcaag gttagagagc ggttcatcca
    10801 ttaaaaacac gcccgcttcg cgcacaatcg cccgcccgag cgccacacgc tggcgttgcc
    10861 cgccggaaag cgcgcccggc ttgcgtttca ggtactcacg caggccgaga atttgcgccg
    10921 cccagttcac ccgctcatca atcacctctt tggcgatttt ttgcatcttc aggccaaacg
    10981 ccatgttgtc gtaaaccgtc atatgcggat acaacgcgta gttctggaac accatcgcga
    11041 tattgcgtgc tttggctgga acgtcattca ttcgtttgcc gtcgatcaac agatcgccgc
    11101 cgctgatctc ctcaagcccg gcaatcatgc gcagggtggt cgatttaccg cagcccgacg
    11161 ggccgacaaa tacgatgaac tctttatcgg caatttccag gttgaagtcc ttcaccacat
    11221 gcacctggtt atcgtagatt ttttgaatat gttgtaacga aagctgagcc atgttgattc
    11281 ctttgctata cgttttgcca gaaggccgat aaccgcgacc aggtcaggga gtccgttgag
    11341 ggcaacagta attgcgcccc ggttaagccc gcaccgatcc ccaccgagcg cataccgctg
    11401 gcgttaatgg cgtcaatgcc cgcctgcgca tcttcaatgc cgatacatgc ctgcggcggc
    11461 acgcccagcc cggcacaggc agcgagaaag atttccggat ccggtttcga gtttttaagt
    11521 tgggaagcat ccgcgcagaa ggtgaaaaac tcgcgcagct ccagcgccgc taaaatcgtc
    11581 ggcgcattca gggagacgga agccagccct accggaatct gctgtgcacg gagctctgcc
    11641 agcaaattgc gaataccggg cagcacagca ttaaccgtca gctcgcgcag tgagtggacg
    11701 taaagcagat ttttgcgata cgccagttgc gcccgctcct gcgggttaaa gtcgccctct
    11761 ttgcccccgt gttgcagaat gcgccgcaga gactcatcgc ggctgatccc ttttagagat
    11821 tcgttaaact gcgcatcaat actgatgcca atttcagcgg caatctgctg ccacgcctgg
    11881 aaatgcagat gcgcggtatc ggtgattaca ccatccagat cgaaaattac cccttgcagt
    11941 ttcattcatc ctcctgatgc gtggtagcgg tcccattgaa gggcaaaata aaatcaccca
    12001 aacagaaaac agattcttca gccacggata taagctgacc gttcaaacgc agtgaaacgg
    12061 gcgcagaagt tcgaatcgca atacgctgcg cgtcgagagt gacctgtaat tcgcagccct
    12121 gccagaacag agggaaagac aactgttgcc actgctcagg taacgccgga tttagatgca
    12181 attcaccgtc acgcacgctc accccggcaa aaccctgaat cgcccccagc cagatagcgc
    12241 cagttgcggc agcatggata ccatcatcac aactatgcgg atcagcacca agatcgattt
    12301 cagtcccctc gcgccagaac tgataacttt gggtcagcag gccacagcgt gcagcaacaa
    12361 tgccgtggat tgctttactt aatgacgagt cgtgaatagt gcgcggttca taaaattgca
    12421 gattggcaag acacgatgcc gatgagaact gctctggcag catgtaattg agcatcacca
    12481 catcagcttg tttgaggatc tgcatctcgt tcacttctgc gcgtgaataa tccagcagaa
    12541 tggtttgctt ccccgccgcc gctttgtatt tcgccagatt aatcgccggc ttagtcataa
    12601 acgaatcatc ctgcggcaaa acaccgtcgg gctgaatttc tggcagccgc agttctttaa
    12661 ggaacatttc ggcacgatga ataaacgcgt cgtcactaca gccaaactgg cgggcaatac
    12721 tcagcgcctg ctggacgttg taatacacca tatagctggt gaaggcgtta ttattgacat
    12781 gttcggtata ttcgtctggc ccaataacat catgaatttc cagacggtcg ttaaccctca
    12841 ccgcgcggct aatccagaac tttgcagttt ccagaagtag cgccatgcct tcatgcgcaa
    12901 tgaaactttc atcccccgtg gtctgccagt attgaataac cgcccaggcg atatcggcca
    12961 ccagatgatg ttccgcctgc gccgaggcca ctttttgccg cagcccggtg cgaatgttaa
    13021 tggcggcaaa ttccggcgtc tcttcttcgc cgctgcgcgc gctttcccac ggaaatagcg
    13081 cgccctgcca gccgttgcgt cgcgctttct cctgcgcgcc tggcaagttg tgccagcgat
    13141 aacgcagtaa acttcgggca accgtcggat cgctaaacag atggaacggc agcaaaaata
    13201 cttctgtatc ccagaaaacg tggcctttgt agccttcgcc ggttaagcct tttgccgcaa
    13261 tactgctgcg ctcgccgtga gccggcgtca tgatgcgcag atgataaagc gcataatcta
    13321 acgcttgctg atcgtgcgca tcgccgccat ttaccgtgat acgacgtttc tgccaccatt
    13381 gacgccagtt ttctgttgat gctgcaagat gttggtcgta actctgttgc gcgcacattt
    13441 cgagtaggca gcctgacggc tgcggcttgt catggtctgc aattaccgtt at
//
LOCUS       DAJTYG010000097        13409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTYG010000097 DAJTYG010000000
VERSION     DAJTYG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.097"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            127..1479
                     /gene="celF"
                     /locus_tag="OO568_003138"
                     /gene_synonym="chbF"
     CDS             127..1479
                     /gene="celF"
                     /locus_tag="OO568_003138"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6642396.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RYTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKNELVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1492..2250
                     /gene="chbG"
                     /locus_tag="OO568_003139"
     CDS             1492..2250
                     /gene="chbG"
                     /locus_tag="OO568_003139"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6642397.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2297..4558)
                     /gene="katE"
                     /locus_tag="OO568_003140"
     CDS             complement(2297..4558)
                     /gene="katE"
                     /locus_tag="OO568_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6642398.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGHVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4741..5004
                     /gene="cedA"
                     /locus_tag="OO568_003141"
     CDS             4741..5004
                     /gene="cedA"
                     /locus_tag="OO568_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6642399.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5281..6096
                     /locus_tag="OO568_003142"
     CDS             5281..6096
                     /locus_tag="OO568_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642400.1"
                     /translation="MRREMFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKLGAPALSFDTYLQDNVKYRVIINNLYGS
                     EFRIRDINEDFVGMDFTCEFRPEKMTNLINIKNRLVIHYLRTIYYEKNYIHPAGSIFA
                     AIQKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLS
                     FSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(6100..7490)
                     /gene="tcyP"
                     /locus_tag="OO568_003143"
                     /pseudo
     CDS             complement(6100..7490)
                     /gene="tcyP"
                     /locus_tag="OO568_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(7623..8213)
                     /locus_tag="OO568_003144"
     CDS             complement(7623..8213)
                     /locus_tag="OO568_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6642401.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(8376..9043)
                     /gene="hxpB"
                     /locus_tag="OO568_003145"
                     /pseudo
     CDS             complement(8376..9043)
                     /gene="hxpB"
                     /locus_tag="OO568_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
     gene            9190..9726
                     /locus_tag="OO568_003146"
     CDS             9190..9726
                     /locus_tag="OO568_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6642402.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(9767..10627)
                     /locus_tag="OO568_003147"
     CDS             complement(9767..10627)
                     /locus_tag="OO568_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6642403.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(10733..11023)
                     /gene="ghoS"
                     /locus_tag="OO568_003148"
     CDS             complement(10733..11023)
                     /gene="ghoS"
                     /locus_tag="OO568_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6642404.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(11124..12053)
                     /gene="pfkB"
                     /locus_tag="OO568_003149"
     CDS             complement(11124..12053)
                     /gene="pfkB"
                     /locus_tag="OO568_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6642405.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVSRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            12339..13093
                     /locus_tag="OO568_003150"
                     /pseudo
     CDS             12339..13093
                     /locus_tag="OO568_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
     gene            complement(13146..13253)
                     /locus_tag="OO568_003151"
     CDS             complement(13146..13253)
                     /locus_tag="OO568_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642406.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT         3437 a         3114 c         3337 g         3521 t
ORIGIN      
        1 attgacggaa tttaatcagt aagtcgttat acctgacaat tcacatatct gtcctgttgc
       61 tggattattt atgtccgggg gggcagatat accagatatc agtattctgt actgaaggga
      121 gaaattatga gccagaaatt aaaagtcgtc actattggtg gcgggagcag ctataccccg
      181 gagttactgg aaggatttat taagcgttat cacgaattgc cggtcagcga attatggctg
      241 gtggatgtcg agggtggcaa agctaaactg gatattattt tcgatctctg ccaacggatg
      301 attgataacg ctggcgtccc gatgaagctt tataaaacgc tggattgccg cgaagcattg
      361 aaagatgctg atttcgttac tacccaactg cgcgttggcc aattaccggc gcgtgaactg
      421 gatgaacgta ttccattaag tcatggttat cttggtcagg aaaccaacgg cgcgggcggt
      481 ttgtttaaag gtctgcgtac cattccggtg atttttgaca tcgtaaaaga tgtcgaagaa
      541 ctttgtccga atgcatgggt gattaacttc actaacccgg cgggaatggt tactgaagcc
      601 gtttatcgtt ataccggatt taaacgcttt atcggcgtgt gtaatattcc gatcggcatg
      661 aagatgttta ttcacgatgt tctgatgctg aaagacagcg atgatttatc tatcgatttg
      721 tttggcctca accatatggt gttcattaag aatgagctgg taaatggcaa gtcgcgcttt
      781 gccgaattgc ttgatggtgt ggcatccggg cagttaaaag catctggcgt taaaaatatt
      841 ttcgatctgc catttagcga aggcttaatt cgttctctga atctgttgcc gtgttcttat
      901 ttgctttatt acttcaagca aaaagagatg ctggctattg aaatgggcga atactacaaa
      961 ggcggcgcac gagcgcaggt agtacagaaa gtcgagaaac aactttttga gctgtataaa
     1021 aacccggagt tgaaagttaa gccgaaagaa ctggaacagc gcggtggggc ttattactct
     1081 gatgcagcgt gcgaagtgat caacgctatc tacaacgaca agcaagctga acattacgtt
     1141 aatatcccgc atcatgggca tattgataat attccggcag actgggcggt agaaatgacc
     1201 tgtaagctgg ggcgcgatgg cgcgacgcca catccgcgca ttacgcattt cgatgataaa
     1261 gtgatggggc tgattcacac cattaaaggc ttcgagattg ctgccagcaa cgccgcactt
     1321 agcggagaat ttaacgatgt gttactggcg ctaaacctta gtccgttggt gcattccgat
     1381 cgcgatgctg agctgctggc acgcgagatg attctggcgc acgagaaatg gctgccaaat
     1441 tttgccgact gcatcgcaga gcttaaaaaa gcacattaac cgaggctgat tatggaacgc
     1501 ttactgattg ttaatgccga tgattttggc ttaagcaaag gtcagaacta cggcattatc
     1561 gaggcctgtc gcaatgggat tgtcacatcg acgacggcgc tggtgaatgg gcaggctatt
     1621 gaccatgcgg tgcagttgag tcgtgatgaa ccgagtctgg ccatagggat gcactttgtc
     1681 cttactatgg gcaagccact gacagctatg ccggggttaa cccgcgatgg tgtgctggga
     1741 aaatggatct ggcagttggc agaagaagat gctttaccgc tggaagaaat tactcaggag
     1801 cttgccagtc agtatttgcg tttcattgag ctatttggac gcaaacctac gcatcttgat
     1861 agccatcatc atgtgcatat gttcccgcag attttcccga ttgttgccag gtttgcggct
     1921 gaagagggga ttgcgttgcg catcgaccgt cagccgctat ctaacgctgg tgatttaccg
     1981 gctaatctgc gcagttcgca gggattcagt agcgcgtttt atggtgaaga gattagcgag
     2041 gcactgttcc tgcaagtgct ggatgacgca agccatcggg gtgatccttc gctggaggtg
     2101 atgtgtcatc cggcgtttat cgataatact atccgtcaga gtgcttactg tttcccacgg
     2161 ttaacggagc tggaagtgct gacatcagcg tcgttgaaat atgcaattgc tgagcgtggt
     2221 tatcggttgg gtagttatct tgatgtgtaa gtaaggtggt aaggtcactc cccaggggac
     2281 gtgaccttat ccatatttac gccggaattt tgtcaatctt aggaatgcgt gaccacacgc
     2341 ggtgttctgc catcagcgtt aacagttcat ccataaaact accgtcggtg ctgtcagctt
     2401 ccacaatccc ttcttcaccc tggtcagcta ccttgattgt tgctttaaac ttgcgcgcgt
     2461 ctcccgccag cgcaatcggt ttaaggtgtt tgtaggcttc catcagatag tagttggcat
     2521 cgccgttgtc agcgatatcc gcgatattgc cgcaagggac aatgaccgca tcgaccgtca
     2581 gcgaaggtgc accggcaaag gtagcggcta taggcaacac cgtaccgtca tccgcagtca
     2641 cttcacccat tcgggagtag agcagtttgg catgaacgcc tttggccttc agggccttga
     2701 gaatggcaag aaggtctgcc gatctcactt catcattaag taaaatcgct accacgtgac
     2761 ctttcacatc accgtcagga atggcgtaca aacttaagga tggatccttt ttcagaccgt
     2821 tgacgtccgg aggtggggtg atattcagct ggtcgtcagt cagttcgata ccgagatttt
     2881 tcgccaccgc ctgggccaga gtgagatcaa tatgcgccag ctggtcaaca acgcgctcac
     2941 gaatatacgg acgaacgact ttgcttaact caaaactgaa accatcgaca atatggcgct
     3001 gctcgaatgg cgtctgactt agccagaaca gacgcggatg ggaataatat tcgccaaacg
     3061 atgggctgcg ctcgcgaact ttattgcctt ccacgcgctc ctggtatgat tcaaaaccgc
     3121 cgcgtttcgg ccccggtggt gtttcgcgcg gccagttatc gttgatcgag ttcggttcat
     3181 aattcgccgg gttagtgtcg atccccatac gatgcatgcc gtcacgctgg aaattatggt
     3241 aagggcaggt cgggcggtta atcggaatct catggaaatt cggcccacca agacgactga
     3301 tttgtgtatc ggtataggag aacaaacgtc cctgcaacag gggatcgttg gtgaagtcca
     3361 gaccaggaac aatatgacct ggatggaatg ctgcttgttc gttttcggca aagaagttat
     3421 ccggattgcg attgagcacc attttgccga cacgctgaac gggcaccagt tcttccggga
     3481 tcagtttggt tggatcgaga agatcgaagt cgaacttgaa ttcatcttct tcaggaatca
     3541 actggaagcc cagttcgtat tccggaaaat cgcctgcttc aatggcttcc cacaactcgc
     3601 ggcggtggaa gtccgggtca cgtccggtga gtttttgtgc ttcatcccaa acgagtgagg
     3661 ctttacctgc cagtggtttc cagtggaaac gtacaaacgt tgccttccct tcggcattaa
     3721 tcaggcggaa ggtgtgaata ccgaagcctt ccatggtgcg gtaactgcgg gggatgccgc
     3781 gatccgacat cgcccacatc acgttgtgca gagtttcagg ttgcagagaa acataatccc
     3841 agaaagtatc gtgggcactt tgcccttgtg gaattgccca gtgcggttct ggttttaccg
     3901 catgaacaaa atcggggaat ttatgcgcat cctggataaa gaagattggc gtgttattgc
     3961 caacgaggtc aaaaataccc tcttcagtat agaacttggt ggcaaaacca cggatatcac
     4021 gcacggtatc ggcagagcca gcgccaccct gaacggtaga gaacctaaca aatactgggg
     4081 tgattttgtt cggatctgag aggaaatccg ctttggtgat atcgcttaag cttttatatg
     4141 gctggaaata accgtgagct gctgatccgc gtgcatgaac aatacgttcc gggatgcgct
     4201 catggtcaaa gtgggtgatt ttctcgcgca gaataaaatc ttccagcagc gttggaccac
     4261 ggttaccggc acgcagtgag ttttgatcat cggcgatgcg cacgccctga ttagtggtca
     4321 gcgcataatt ttcactgcct ttgcgtacgt cttccagaga attaagtttt tcgttacgtg
     4381 tatcaggggc tttcaggctc ccaggggcgg taggttgtgc acctggcggt gttggttcag
     4441 ccgctggacg atgagatccg tcctcaggtg ccagtgagtc catccccggt ttcgcttcgc
     4501 tggaatcgtg tagtggtgac tggtgctgat gtgggttctt ttcgttatgt tgcgacattg
     4561 aactcgtctc cttaatttat tactggaagg gccgcttttt tggtcaaaaa actctctaac
     4621 tatagaccac cagccagatc ccgcttcgga gactattctg gaaacggtgt tatgaaatac
     4681 acgcaaagcg ggacgtacag gggctaaatc ggctaaacta cagtttttct aatttttaat
     4741 atgcgtttag tgaagccagt aatgaagaaa ccgctccgcc agcaaaaccg ccagattatt
     4801 agctatgtcc cacgcacgga acccgcgccg ccagaacatg cgataaagat ggattcgttt
     4861 cgtgatgtct ggatgctgcg tggcaaatat gttgcgtttg tactgatggg agagtcattt
     4921 ctgcgctcac cggcgtttac tgtgcctgaa tctgcccaac gttgggcaaa tcagatccgc
     4981 caggaagggg aagtgactga gtaaggatta catacctcct ggttttgccg ggagttacag
     5041 gacctgaaat tctgcatttc gttgctacat tcattcaaac cacctgtaac aaaaagctgc
     5101 ctttgacgag ctattgctat aaataagtga tcttttttct ttaattgttt atttatttta
     5161 aaatggttaa gttttgctaa tcatgatgag ttatttttga tagttcatcc tgtggcgtcg
     5221 ctcttaaact tatattgtaa atggcttaga tttaaataag ataataattt tgttaacaat
     5281 atgaggcggg agatgttttc tacaaactta atacagagta attatggtga tttaaatatt
     5341 aaaagcctgg ctttcgactc ttttaaagaa aggcttgagt ctaccatggc ggcactcact
     5401 ttctttatat caacgggtca atgtgactgc gatgaaatag cagagtcgaa cttcaatcgt
     5461 atgattgcct atatgtctaa cattaactat gatgcctcaa agctaggagc gccagcactt
     5521 agttttgata cgtatctgca ggacaatgtt aaatatagag tgataattaa taatctttat
     5581 ggcagtgagt ttcggataag agatattaat gaggattttg ttgggatgga ttttacctgt
     5641 gaattccgac ccgaaaaaat gacgaatttg atcaatataa aaaataggtt ggttattcat
     5701 tatttaagga ctatatacta tgaaaaaaat tatatacatc ctgccgggag catttttgct
     5761 gcgattcaaa aaaatgagag tctcctaaag tttccttcga taatatcaat gcttaatatc
     5821 aatcttcttt ttaatccact aaatcttccg ggaatgggaa gtggtgtgct tgaagatatt
     5881 atgagtattc cagatagttc acttagaaaa cgtctcgggt atgaggtttt aagcttctca
     5941 ctacaggcac attcattgag ccaggaatgt attgataagt tggacatttt tttcgctgac
     6001 gatctgttta aatatgagtc agtatgcatc gccgcgatgg aacatttaaa aagtaaagca
     6061 acggccccta tacaaaacgg accgttgcca gcataagaat taacggtgtg ccagttcggc
     6121 gtcgtcttcg ctatccagaa tggctttatc ggtttgcttc agccactggc tggtcagcgt
     6181 gccagctgtc atcgagccac taacgtttag cgccgtacgg cccatgtcga taagcggttc
     6241 aacggagatt aacagcgcca ccagggttac tggcaggccc atcgcaggca gcacaatcag
     6301 tgcggcgaaa gttgcaccac caccgacacc ggcaacgcct gcggaactaa cggtaacaat
     6361 accgaccagc gtcgcaatcc acatcgggtc cagcgggtta atgccaaccg taggcgcaac
     6421 catcaccgcc agcattgccg gatacaaacc ggcgcagccg ttctgaccaa tggttgcacc
     6481 gaaagaggcg gcgaaactgg cgatggattc aggaacgccc agacgacgcg tttgtgcttc
     6541 cacattcagt gggatagacg cagcactgga acggctggta aaggcaaacg tcagcacagg
     6601 ccatacctta cggaagtact tcagcggact cacgccatta atgcccagca gaatgccatg
     6661 cacagcaaac ataatcagca gaccgaggta ggacgcgaca acgaaacttc ccagtttgat
     6721 gatgtcttgc aagttagaac ctgcaaccac tttggtcatt agagccagaa cgccgtaagg
     6781 ggtcaactgc atgacaaggc gaaccagttt catcactcag ctttgtaggg tatcgatagc
     6841 ggttaagacg cgttcacctt tcggcgcatc atccttcagc agtttcaatg cagctacgcc
     6901 gaggaatgcg gcaaaaatta ccacgctgat aattgacgtc ggattggctc cggtaagatc
     6961 ggcaaacggg tttttcggga taaaggacaa gaccagctgc ggaacgctca gatcagagac
     7021 tttaccaaca tagttacttt caatggcgtt cagacgtgca gtttctgcac caccctgaac
     7081 cagaccttca gccgtcaaac caaacaggtg gtgaccagca caccgaccag cgccgcaatc
     7141 agcgtggtaa acaaaagcgt accgatggtc agaaaactga ttttgcctaa ctgagatgcg
     7201 ttatgcagac gggcaaccgc gctcagaata gaggcgaaga ctaacggcat aacgatcatt
     7261 tgcagcagtt gaacattgcc gttaccaacg atgttaaacc actgtacaga atctttaagt
     7321 acctggctgt cagaaccata aatggtatgc agggcaaggc caaaaaccac acccatcacc
     7381 agacccacca gcactttttt cgccagactc cactgtttat ggcgggtctg agccagcgca
     7441 aacagcagta caacgaacac cacgatgttc gcaattaatg gaaagttcat ccccgttctc
     7501 ctgatcttat tatttctatc actgcgtagc agtcatagtg ttggcgcaag attaacagaa
     7561 ctgtgagctg gctcttatat tcaaatggaa ttgcttattc cagaaaggtg atttttgcct
     7621 ttttagtatt caacctgatg cttaataagc cgatgaaact ggatctgcaa ggtattgatc
     7681 atttgcgatg accaacgaac agcgctgttc tcgggtaatg aatggggcat ccataccgac
     7741 cagacaaata atgccatgca gataaaacgt tccagttggt tgcccttttg gggaaccagg
     7801 ataggcaagc ggaaacgcca gcggcatggc cagagcaggg gaacaccggc gggtgtcagc
     7861 atatcggcaa gtatgtggct caaataaccc agcaccattc cttgtagcgc atcagccgga
     7921 atgaaccagc cttccggaac cttaaggtaa aaggttgcca gcagcgcaaa taccgccagc
     7981 agactgtggg taaaaccacg atgcccaaaa gcgcgggcga tcggttttga tatccatttt
     8041 aatcgctgcc caagaaacga ctttgggtga tcgatgtccg gtaacaaaca cgtcaggatt
     8101 gcggaaggga caatatgcca ccagtcaccc tgtgccagca cgggcgtcag ctcggcattt
     8161 ttggcaaata ccgcacaagc aatagaaaag agaaggtgac cttccgccgt catgataaaa
     8221 ctcactaaac tgtcgatacg tacagtatag gatttttata cagtgcacgg aagtggtgac
     8281 ggtgtaactc tttctcttaa attgacgatt aatccgtcag ttgaatgcaa attcagataa
     8341 agaaccaggg gcagcgtgat cactgcccct ggtcattaac cgagaaggtc ttttgcggtg
     8401 agttctgtca gcgatgagag tttgacgttt gctaatacaa aacgtggatc attttgcgct
     8461 tctggcgcag gaacgacgat ggaacgcatg caggctgctt tagaagcgat catgccattt
     8521 accgagtctt ccagcgccac gcaggtcagt gggtcaacgc ccagttttgc tgcgcagtcg
     8581 agatatactt gcggatgcgg cttgctgtaa ggcagttttt caaccgaggc gagggcatcg
     8641 aaactgtcgc gtaagtcaaa catggtcaac actttttcca gcatatgtag tggtgacgcg
     8701 gaggccagtc ccaccaataa accttgttct ttgcataacg caacagcttc gcgcacgcct
     8761 ggtaataatg gacgtgtctc ttcaaccagt gaaatggcac gggcaataac ccgttctact
     8821 acttcctgac ggcttggccc attccacggt tgccgggcgt acaaagatcg accaccatat
     8881 cgatgcgtaa acctaaggtg tccggcagct cgttacgacg ggagatatct acccccaggc
     8941 ttgccatcac atccagttcg gctcgatccc ataaaggttc tgagtcgata agtaatccat
     9001 ccatatcaaa aattgcagca agaatctgac gcggggttga cattacaacc tctcctttag
     9061 agcattatgt gcaaggtatt aagccgtgct tatgattaag cgcctatcat atcgccttta
     9121 aagaatgggc gaaaacagat atctacaggt aaactaagtg aaacgacgcg tcttaatcaa
     9181 ggagaactca tgacgtatca acaagctgga cgcattgctg ttttgaaaag gattttgggt
     9241 tgggtgattt ttatacctgc attgatctct acattgattt ctttgctgaa atttatgaat
     9301 acccggcagg aaaaccagga aggcattaat gcggtcatgc tcgactttgc tcatgtcatg
     9361 atcgatatga tgcaggcgaa tacgcctttt ttgaatctgt tctggtataa ctccccgacc
     9421 cctaatttta atggtggtgt gaacgttatg ttctgggtga ttttcatcct gatttttgtc
     9481 ggactggcgt tgcaggattc cggtgcccgg atgagtcgcc aggcgcgctt cttgcgggaa
     9541 ggtgttgaag atcaactcat tctggaaaaa gccaaagggg aagaggggct aacgcgtgaa
     9601 caaattgagt cccgtattgt tgttccgcac catactattt tcctgcagtt tttctcacta
     9661 tatatcctgc cggttatttg tattgctgca ggttatgtgt tcttttctct gcttgggttt
     9721 atttaatcaa aaggccgcac ggagcggcca ttatcctcaa cccatatcat gctgctaata
     9781 atctatccaa tgactgctga gcaataacca aatgctgacc gccaaataat cttgcacgat
     9841 ttaacagcgt gtagagttgg taaaccggtt gacgttcaag gaaatcggca ggtagcgggg
     9901 atactgactg atagccgtca tagatttgtg gcggctgttc agtatgcagc ggtaacatcg
     9961 ccaggtcgca ctctcggtca ccccagtagc aggcagggtc gaaaatgtac gggccatccg
    10021 gacccagcgc acagttgccg gaccataaat cgccgtgcaa cagagaaggc tgcggttgat
    10081 gcgaggccag acgctgctga atatgctcga cgatggcgtc gatattgccg aaagcgatcc
    10141 ctttctctgc tgccagttcc aactgccagc caatccgttg ttcagcaaaa aacgttgacc
    10201 agcgacgttg ccaggtgttg ggctgtggag ttgtggagag cgagttatca aaatcgaggc
    10261 caaattgtgg ttggtcactc cattgatgta aacgcgcaat ttgctgacca agaataaatg
    10321 cgctatgcgc atccagcgga cgaggtggga gataatccat caccagaaaa ctgtagtcac
    10381 ggtcagcgcc tactgcccaa accttaggca cggtgacggt tttactacgc gacagtaact
    10441 ccagttggtc ggcttcggcg gtaaaaccgg gaagcagttc cctttcatca catttgacga
    10501 aaaagtcatg tcctgcatag cgcaaatgcc atgcggcatg gacttctccg ccaggcagtt
    10561 cattacgcag ttcgatttcg ccttcaccta actgctcgct caaaagacga ctgattgcct
    10621 gccacatgat gtctctccca tgttgtctgc cagatcataa ggttagcgca agaatgcggt
    10681 gtaaaaacac gagctaacgc acaccacaga ctgattattg cccaaaaacc atttattttc
    10741 tgttgctatt ccattcctcc caggtcgtga tggtgatttc aggatctttg ccggttgcac
    10801 tttgggtaag ccccgaaacc agttctctaa tttcttcttc actttgggta ctgataagtc
    10861 caaaagtgtt agtccccagc tcatggatat ttccctcatc atccgtcatg gtgagcaaaa
    10921 aaccgtcgcg agtcaggtaa ttattcaact cgttaatttc agtcaatgta tcctcatgca
    10981 acatgacggt tatgacataa cggacaagat cgccactggc catagttcac ccctttgtta
    11041 tcacatgact tttgagcata gtcggagaaa cgcgttgccg acaggttggt ggtgattccc
    11101 ccaatgctgg gggaatgttt ttgttagcgg gaaaggtaag cgtaaatttt ttgcgtatcg
    11161 tcatgggagc acagacgtgt tccctgattg agtgtggctg cactccccgc agctacgcca
    11221 aaacggacca tctcttcaag agaggcattt tctgccagtt tcagtgtcat cgcgccgacc
    11281 atgctgtcac cagcgccaac ggtactctgg cttttcaccg gtggtggcac cacctgaatg
    11341 cagttttcac tatcaacacc cagcgctcct tgtggaccaa gggaaacgac aacccgtttg
    11401 gccttgccgc tattaacgat ttcctgcgcg gctttgcgga catcgtctgg ctgggtgagt
    11461 tcgcgactca ccagcgcact gagttctttt tggttaggct taaccaactc gatgttacca
    11521 atcgccagtg ctgcacttaa cgcttcgcca gaactgtcga tgatgcagcg gatcccttgg
    11581 ttttgcgcag cggaaatcag ttgggttaat ttttccagct tcacacctgg cggcaggctt
    11641 ccgcttatga ccaggatggc cccggattca atttccagaa cttgctcttc aagctggcga
    11701 aactcatctt catttaatgc cgcgcctggc ataacaaaac gatactgttc accgcttgct
    11761 tccacatgta cgtgtaaatt ctgccgggtc cagtctttgg cttctacagt agcgacgggg
    11821 acattttcat ccgccaacag tgaaaccagg tgttcgccgg tcgcgccacc cgccgggaag
    11881 atcgctgtgg cactgcctcc aagatgggca atggcgcggg cgacgttgat gccgccgccc
    11941 ccgggttcga acaccggtgc ggtacagcgc agttttcctt cgggataaat ttgcggggta
    12001 attgttgcgc tatcgagaga gggcgcaagt gtcaacgtat agatacgtac catcatttcc
    12061 tcctataggc tgatttcagt ctggcaccga atcagcggaa agtgaagaaa ttaacaatat
    12121 gatttattag agctttattt aaaattttgc agataaatat atataaataa aaatctgttt
    12181 tcgccacatg cttattcaaa aaatgaaata agaatacatt gctaagttat tcgcgccttt
    12241 ttataattcg ttacctttcc agggacgcta atctttgatc cttaagaaag tttccgggac
    12301 cgtgatggtc tcttttttgt tgtacggact cttgataaat gaagcttttg aagacagttc
    12361 ctgccatcgt tatgttggcg gggggcatgt ttgcgtcact gaatgcagct gccgatgatt
    12421 ccgtttttac tgtcatggac gatcctgcca gcgctaaaaa acctttcgaa ggtaatctga
    12481 acgcaggata tcttgcacaa tcaggcaata cgaaaagctc ctcacttacg gctgacacca
    12541 ccatgacctg gtatggacaa accactgcct ggtcgctgtg ggggaacgcc agtaatactt
    12601 cttctaatga tgagcgttct tcagaaaaat atgcggcggg cgtagccgct ttaatttaac
    12661 tgattatgac tatttatttg gtcaggcaag ctggctgaca gaccgttata acggctatcg
    12721 agagcgcgat gtgttgaccg cgggttatgg tcgtcagttt ttgaatggcc cggtacacag
    12781 tttccgcttt gaattcggtc caggtgtgcg ttacgacaaa tataccgaca atgccagtga
    12841 aacccagccg ctgggctatg cctccggggc ttatgcatgg cagttgactg acaacgctaa
    12901 atttacgcag ggtgtttcgg tctttggcgc ggaagacaca acgctgaact ccgaaagcgc
    12961 attaaatgtt gctattaatg aacattttgg actgaaggtg gcttacaacg tcacatggaa
    13021 ctctgaacct ccagagtctg caccggaaca taccgaccgt cggacaacct tatcgctggg
    13081 ctacagcatg taaaattatc tgccggaccg tgttatgcgg tctggctgtc ttcgtttgtc
    13141 actgctcagc cccaaatcat tgctgcccaa cgtagcaaca acatagcgcc acagattgcc
    13201 agtagcacca ccaccatctt ccagtgtttt ttatcaaagc gtttagtcgg cataccagtg
    13261 tcctgcatat ctcttgtgcg tgataacgga gtgtaacaaa agggacaggc atcaagggat
    13321 tttttgttat tacagccgtc gggtggcgtt gtgcacatgg tcactgtttc tgcgttttat
    13381 gaaaaaaata aaattatgta tcgatatat
//
LOCUS       DAJTYG010000098        13302 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTYG010000098 DAJTYG010000000
VERSION     DAJTYG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.098"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            63..383
                     /locus_tag="OO568_003152"
     CDS             63..383
                     /locus_tag="OO568_003152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642407.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(449..681)
                     /locus_tag="OO568_003153"
                     /pseudo
     CDS             complement(449..681)
                     /locus_tag="OO568_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(791..1561)
                     /gene="yafV"
                     /locus_tag="OO568_003154"
     CDS             complement(791..1561)
                     /gene="yafV"
                     /locus_tag="OO568_003154"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6642408.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            1742..2188
                     /gene="ivy"
                     /locus_tag="OO568_003155"
     CDS             1742..2188
                     /gene="ivy"
                     /locus_tag="OO568_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6642409.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2231..4675)
                     /gene="fadE"
                     /locus_tag="OO568_003156"
     CDS             complement(2231..4675)
                     /gene="fadE"
                     /locus_tag="OO568_003156"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6642410.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADSIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4914..5492
                     /gene="lpcA"
                     /locus_tag="OO568_003157"
     CDS             4914..5492
                     /gene="lpcA"
                     /locus_tag="OO568_003157"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6642411.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5698..6465
                     /locus_tag="OO568_003158"
     CDS             5698..6465
                     /locus_tag="OO568_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6642412.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6436..7176)
                     /locus_tag="OO568_003159"
     CDS             complement(6436..7176)
                     /locus_tag="OO568_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6642413.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            7560..8081
                     /locus_tag="OO568_003160"
                     /pseudo
     CDS             7560..8081
                     /locus_tag="OO568_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            8257..8754
                     /gene="rayT"
                     /locus_tag="OO568_003161"
     CDS             8257..8754
                     /gene="rayT"
                     /locus_tag="OO568_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6642414.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(8971..10683)
                     /locus_tag="OO568_003162"
                     /pseudo
     CDS             complement(8971..10683)
                     /locus_tag="OO568_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            10655..11440
                     /gene="lafU"
                     /locus_tag="OO568_003163"
     CDS             10655..11440
                     /gene="lafU"
                     /locus_tag="OO568_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6642415.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            11511..12566
                     /gene="dinB"
                     /locus_tag="OO568_003164"
     CDS             11511..12566
                     /gene="dinB"
                     /locus_tag="OO568_003164"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6642416.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            12618..12911
                     /gene="yafN"
                     /locus_tag="OO568_003165"
     CDS             12618..12911
                     /gene="yafN"
                     /locus_tag="OO568_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR6642417.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            12914..>13302
                     /gene="yafO"
                     /locus_tag="OO568_003166"
     CDS             12914..>13302
                     /gene="yafO"
                     /locus_tag="OO568_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR6642418.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFR"
BASE COUNT         3227 a         3468 c         3430 g         3177 t
ORIGIN      
        1 gttcatgggc aaattcttgg aaataaagtt agccgatgta attagttagt ggagaggaag
       61 ttgtggacga aattagaata agaattgctg gtaatacggc tgcgccttgt ttccatgtgc
      121 taaaaaataa gggatataaa gtaacaatgg aaactcatgc attatctgac gattgtttag
      181 attgtgtata tgattggtat gcggaaaaaa atggacaatt ttttagtgca acgagtccag
      241 aagagttact tggattagtt gcaatgtggg aagttcgtgg taatgactgg agagctaatt
      301 tgaatgagat aaatacttat caaaaagcca tagaagaatc cccgtttttt gataaaaacg
      361 gcaatgaaat agagtctgac taaattttat tgatatagca acgataataa ccggaaattt
      421 cttccagatc tttccggctt tttattattt aactttcgtt cagcacacta aacatcccgt
      481 ctcgcatccc cttaactgac tgtttaacct gataaagttc cctgcgcagt tcctgtactt
      541 cgttgttatc ccaagcagga tcaaacatcc ctccgagatc ttcacggtaa cgcccgtgtc
      601 gatctcaaat cccgcttttc tcagccattt ggcgctgatt ttgatcaccg gcgtgtcgct
      661 gcggttgggc agataaccca cggtacagcg acgctgtgtt ttggaaatgg tgacttctgg
      721 cgtaatattg tgtttaagtt aggggctttc gtaatctagc ccagcccccg gccctaaaca
      781 caacccccac tcaccacaac ctaaactcat ccgcatcccg ccatgccgga aacttttctc
      841 tatattcccg caatgccacc atcgacagct ccgcatcaat acgcgttgcc ttatgcgcgt
      901 cggcggtagc gataatctcg ccttgcggat taatcacccg gctgtcaccg cgataatggc
      961 agccgttgcc atcgctgccg acgcgattgc atcccgccac atacgcctga ttctcaatcg
     1021 cgcgggccgt cagcaatgcc tgccagtgca gagagcgcgg agctggccag ttggcgacgt
     1081 acagggcgag gtcataatcg ttgagattgc gcgaccacac tggaaaacgt aagtcgtagc
     1141 acaccagcgg caaaatacgc cagccgcgcc attccacaat cactcgcgca ttgcccgctt
     1201 tataatgcag atgctcatct gccatgcgga acagatgacg cttatcataa aaatgtaccg
     1261 tgccgcccgg ctcaaccagc aaaaagcggt taaccgaacc agactccgtt tgtaatgcaa
     1321 cactgcctgc aatcagcgca ttgcactgct gcgccttagc tgtcatccag ttcactacgt
     1381 catcttgtgc aagcgacgaa gctgccgctt ccatggcaaa gccgctggta aacatctccg
     1441 gtagaacgat cacatcgcgc ccggtaatac cttccagttg acgatcaaaa tggcgcaggt
     1501 tggcaggacc atccacccac accaacggtt gctgcaaaag cgtaatcttc aaactaggca
     1561 cggtgtacaa ctcctttatg cgaagggttt tataacttta acaccttatc aggcagttgc
     1621 cttagcgcag aataaattga taacaaatgc tgatattgga aatatctgat ttgcaaatta
     1681 tcgtgttatc gccaggcttt aggaggttaa taacatgggc aggataagct cgggaggaat
     1741 gatgtttaag gcaataacga cagtcgccgc actggtcatc gccaccagtg caatggcgca
     1801 ggatgattta accattagca gccttgcaaa gggcgaaacc accaaagctg cgtttaatca
     1861 gatggtgcaa gggcataagc tacctgcatg ggtgatgaaa ggcggtactt atactcccgc
     1921 acaaaccgta acgttgggag atgagacgta tcaggtgatg agcgcgtgca aaccgcatga
     1981 ctgtggctcg caacgtatcg ctgtgatgtg gtccgagaaa tctaatcaga tgacggggct
     2041 gttctcggct attgatgaga aaacgtcgca agagaaactc acctggctga atgtgaacga
     2101 tgcgctttcg attgatggta aaacggtgct gttcgcggcg ttgaccggca gcctggaaaa
     2161 ccatccggat ggctttaatt ttaaataatt agcggataaa gaaacggagc ctttcggctc
     2221 cgttattcaa ttacgcggct tcaactttcc gcactttctc cggcaacttt accggcttcg
     2281 tcgccagctc ttccggatca aagtcatcaa cgttaatact gcgcagacgg ctttcttccg
     2341 ctttcaccag aatagcggct tcatctttat caatcagccc cttcgtcagc gcgttgtgcg
     2401 ccagttcatc cagacgggta aatggcagat ttttacccag ctctttacag atccgctgat
     2461 gaatcgagtc ggcggcaatc acatccacca acgcctcttc cagtaaacca accgggttat
     2521 gctcgcttgg cgtcaggtac tgaccgcgac caatacgtga acgagtggcg ttcggcactt
     2581 gtaaaatctt cgccacttta tgatccagct tgtcagacgg tgccagataa tgacgtccgg
     2641 tcgggaagat caccacattc agcagcccgg caaccacgcg gttcgggaag ttttgcagta
     2701 aatcgtccat cgcctgttct gcctgataca gcgcatcctg tacgccccag tgcaccagcg
     2761 gcagatcggc ttcattacgg ccttcgtcgt cataacgctt cagaacggca gaggcgaggt
     2821 aaagctggct taaaatatcc cccagacgtg ccgagatacg ctcacgacgt ttcaggctgc
     2881 cgcccagcag tgccatcgag acatcagaaa gcagggcgag gttggcgctc aggcggttca
     2941 ggtgctgata gtagcgttta gtggcatcgc cggttggcgt gctgctggtt aaaccgcgcg
     3001 tcaggcccag ccagaagctg cgaactttgt tgctaccgac gtgaccgata tgtttgaaca
     3061 acagtttatc gaacgcgttg acgtcattgt tcttcgccgc ttccatctct tccagcacgt
     3121 acggatggca acgaatcgct ccttgtccga agatcatcat gctgcgggtc agaatgttag
     3181 ccccttcaac ggtgatggca atcggtgcgc cctggtaagc acgcgccagg aagttgcttt
     3241 gcccgagcat aatgccttta ccgccggtaa tatccatcgc atcaataatc gactgctgcc
     3301 cgcggtgggt acagtgatac ttaacgatag ccgacagcac ggcaggtttt tcgccgagca
     3361 taatgccgta ggtaatcagc gatgccgcag catccattac ataagcgttc ccggcaatac
     3421 gcgccagcgg ctcttcaatc ccttccatct taccgataga gattttgaac tgacggcgaa
     3481 tgtgagaata cgcgcccgtt gccagcgcta ccgatttcac gccgccggtt gagttggaag
     3541 gcagggtgat gccgcggcct accgagaggc actccaccag catccgccag ccttgcccgg
     3601 ccattttcgg cccgccgatt atgtaatcga tcggcacgaa gacatcttta ccgcgcgtcg
     3661 gtccgttctg gaacggtacg ttcagcggga agtggcgacg accaatttcc acgcccggcg
     3721 tggtggttgg gatcagcgca caggtaatgc ctaaatcttc tgcaccgccg agtaattttt
     3781 ccgggtcgga gagtttaaac gccagcccaa gcacggtcgc aatcggtgcc agcgtaatgt
     3841 agcgtttgtt ccaggtcaga cgcatcccca gcacctgctg gccctgccat tcgcccatgc
     3901 agacaatccc ggtgtccgga atcgcgcccg catcggaacc cgcttccggg ctggtcagtg
     3961 caaagcaggg gatctcctga ccacgcgcca gacgcggcag atagtgatct ttctgctcgt
     4021 cagtgccgta atgttgcaac agttcgcccg ggcctaatga gtttggcacg ccgacggtaa
     4081 tcgccaggat cccgctcacg ccagagagtt tttgcagcac acgagactgg gcataagctg
     4141 agaactccag tccgccgtac tcttttttga taatcatcgc gaagaagcga tgctctttaa
     4201 ggtacgccca caactccggc ggcagatccg ccagctcatg agtaatctgg aaatcattcg
     4261 ccatccggca ggcttcttct accgggccgt cgagaaacgc ttgctcttca gcggtcaggc
     4321 gcggctgcgg atagttatgc agctttttcc agtccggctt gccctggaac aagtcgccct
     4381 cccaccaggt ggtgcccgca tcaatcgctt ctttctcagt gcgcgacatc ggcggcatca
     4441 ccttacggaa accgcgaaat accggcgcgg aaatcatcga cttacgcata ggcgcaaagt
     4501 taaatggcac gaggataatg gtcagaggca ccagtaccca cgccgaccac agaccagcaa
     4561 cgccgagggc ggctgtccag gcgagcaaaa tcagactgct gataaataag ctcacgcggt
     4621 gatagaacaa cgcgccgagc aggacaaccg tagcgagaat actcaaaatc atcataacga
     4681 aaagcccctt acttgtagga ggtctgacca ctgtgatgat atggttgtag tggatgtaaa
     4741 aacatttagc aatgtgttta caatataatt acaacaaagc tcacattgtt gctgttttta
     4801 tccgcacttc aggtcaaaaa gtcctggtca tagcacctgc ccgtacttct cgcttttggc
     4861 ggtatccggt acactgcatt ttgtctatta catttatgct gaaggatatc ctcatgtacc
     4921 aggatcttat tcgtaacgaa ctgaacgaag cggcggaaac gctggctaac tttttaaaag
     4981 atgacgccaa tattcacgcc attcagcgcg cggcggtcct gttagcagac agctttaaag
     5041 ccggtggcaa agtgctttcc tgcggcaacg gcggttccca ttgcgacgct atgcactttg
     5101 ccgaagagtt gaccggtcgc taccgtgaaa accgtccggg ctacccggcg attgctattt
     5161 ctgacgttag tcatatttcc tgcgtcggta atgatttcgg tttcaatgat attttctccc
     5221 gctacgttga agcggtaggt cgcgaaggcg atgtactgct ggggatctcc acctccggta
     5281 actctgcaaa cgtgatcaaa gcgatcgcag cggcgcgtga gaaggggatg aaagtgatca
     5341 ccctgaccgg taaagacggc ggtaaaatgg ctggcacggc ggatatcgaa attcgcgttc
     5401 cgcactttgg ttatgccgac cgcattcagg agattcacat taaagtgatc catatcctga
     5461 tccagttaat tgaaaaagag atggttaagt aagtctggcg taggccggat aaggcgttta
     5521 cgccgcatcc ggcacttgtg cgctgatgcc tgatgcgatg ctgacgcgtc ttatcatgcc
     5581 tacaaatctg tacccgaacc gtaggccgaa taattcgttt acgccgcatc cgacctgaaa
     5641 attcttaaat caatcttcgc cggggtccat gcgctcccgc tgttgtggag gttacccatg
     5701 tgcgaattgc tcgggatgag cgccaacgtc cctaccgata tctgctttag tttcaccgga
     5761 cttgtacagc gtggtggtgg aaccgggcca cataaagatg gctggggcat taccttttac
     5821 gaaggtaaag gctgtcgcac atttaaagat ccacaaccca gctttaattc ccccatcgcc
     5881 aaacttgtcc aggactaccc gataaaatcc tgttcggtgg tggctcatat tcgccaggct
     5941 aatcggggcg aggtggcgct ggaaaatact cacccattta cccgcgagtt atgggggcgt
     6001 aactggactt atgcccataa cggacaactg acgggctaca aatcactgga aaccggcaac
     6061 ttccgcccgg tcggtgaaac cgacagcgaa aaagcctttt gctggctgct gcataaatta
     6121 acgcagcgtt acccgcgcac gccgggcaac atggcggcag tgtttaaata tatcgcctca
     6181 ctggcggatg aactgcgggc gaagggcgtt ttcaacatgc tgctttcgga cgggcgctat
     6241 gtaatggcgt attgctcgac taatttacac tggatcaccc gccgcgcgcc gtttggcgtg
     6301 gcaacgttgc tggatcagga tgtggaaatc gacttcagct cgcagaccac accgaatgat
     6361 gtggtcacgg tgattgcgac acagccgctg acgggcaatg aaacctggca aaaaattatg
     6421 ccaggcgaat ggcgcttatt ttgcctcggg gagcgtgtag tttgatgcca gttgtggctg
     6481 caccacttcg tggcttaacg gcttgctgac cacgtaacgg ccattggcca cagaaacggt
     6541 cggtggctta cgggtttgct caaagtagtc gtagcccggc ttcagctgct cccagaagtc
     6601 cttaaagttg gaatatttat ggcgcttcat attggcgtcg gtcatgcgga atgggtaaat
     6661 actcacctgc acgctcggct gaccaaacac cagcgcacca gtaacgaact ggaagatctc
     6721 atcaataccc tgattggtca ttgcgtagca gccgatggat acacaatcgc cgtggatcat
     6781 caggtatttc ccttcataac catgcgcacg gtcataagca ttggggaatc caatattaat
     6841 cgctttgtag taacggctgt ctggttttaa ctgattacgc tggacgctat aaaatccttc
     6901 cgggcttttg aaatcgccct gacgctgttt tggccctaag ccgccggaat atttacagat
     6961 tttataactg tcgagcagtt gatattgctc gcccattttg acgtaaagat cgagcgtacg
     7021 ttcttccttg aagatctgga tgtagacagg ggatcccatc aactgctgct tatactcttt
     7081 gctcactggc gtggtcgagc tactgctgcc cagcaaaccg gcaaacgaaa cgcacgggat
     7141 caacaacatc gcaagaatta atgcgatttt acgcatactg cttattcctt gataaaacgg
     7201 ttacacacgc caggacggca aaatggatcc caaatcggaa tagtctggat ttggaaggct
     7261 cacattatca ccaaaagagt tttacgcaag cctgtcggcg ctgggtttac aaatttagca
     7321 caggggcagt tttcaatagt ccgaaaagcg catgtatcat ttatttccta atattatgaa
     7381 atctcaatag taatatgcaa ataagcaatt aataatcccg tttactcctc cggtctttta
     7441 aaattctcac cttcattcag ccggtttatg tctttcatgt cttcgttctt gctcggcaga
     7501 tttttgcatc caggcgtgtt ttcgttatgc gttttgctcc ctttactcgc cagcgccacc
     7561 acaaccggga gcagataaaa cagcatccag aatggtttcc tgcgccgctc aaggcgagtg
     7621 acagacgctg gcaggagctg gcggaaaaca accacttttt aagcagcgat cacctgcata
     7681 acatcaccga agtggcgatt caccgcctgg agcagcagct tggcaagcct tacgtctggg
     7741 gcggcacgcg gcccgatcaa ggcttcgatt gtagtgggtt agttttttat gcctacaaca
     7801 agatccttga ggcgaaactc ccgcgcacgg ccaatgagat gtaccactac cgtcgggcaa
     7861 cgattgtggc gaataacgac ctgcgccggg gagatttgct atttttccat atccacagcc
     7921 gcgagattgc cgaccatatg ggggtgtatc tgggcgacgg tcagtttatt gagtcactgc
     7981 gcaccgggga aaccattcgg gtaagccggt tagccgaacc tttctggcag gaccattttt
     8041 taggcgcacg caggattttg acggaagaga tgattttgta ggccggataa ggcgtttacg
     8101 ccgcatccgg cggttgtgca cggatgcctg atgcgacgct gacgcgtctt atcatgccta
     8161 caaatctgtg cgtttttgcg agccgctttc ccgatataac acagcgtcat tgtcggcctt
     8221 tccttttcct cttaccgttg agaaaaagga gtcgctatgt cggaatatcg tcgttattac
     8281 atcaaggggg gaacatggtt tttcacggta aatttacgca atcgtcgaag ccatcttctg
     8341 accacccagt tccagacgct ccgtaacgcc attattaacg ttaagcgaga cagacctttt
     8401 gaaattaacg cctgggtcgt tttgccggag catatgcatt gcatatggac attacccgaa
     8461 agcgatgatg atttttcctc tcgctggcgg gagattaaaa agcagttcac ccatgcttgc
     8521 ggattgaaaa atatctggca accgcgtttt tgggagcatg ccattcgcaa tacaaaagat
     8581 tatcgccatc atgttgatta tatttatata aatccagaga aacatggctg ggtaaagcag
     8641 gtgagtgatt ggccattctc aacgttccat cgcgatgtcg cgcgagggtt atatcccatc
     8701 gattgggcgg gagacataac ggatttaagt gccggggagc gtatcatttt ataactgtgc
     8761 gctggtgccg gatgcgctta cgcttatccg gcctacaaat cgtgctttaa cggtaggccg
     8821 gataaggcgt tcacgccgca tccggcagtt atgcgcagat gcctgatgcg acgctggcgc
     8881 gtcttatcat gcctaaaaac ttgtgcctga acggtaggcc ggataaggcg tttacgccgc
     8941 atccggcaat tgtgcaccga tgccggattc ttacatcaac gcccccataa tcttcaactc
     9001 cagctcatcc ggcacttcgt tatacgacag cacatgcagc cccggcgcaa acaaccttgc
     9061 ataacgcgcc agcaaagggc gtagctgcgg cggcaccagc agcaccggat ctttccccgc
     9121 cgctttcatc tgctctttca cctgtggcat cgtgctctgg aactggttga gcatattcgg
     9181 gtcaaccggc acgctgtcga gcatcacttt gccggcctgt tgcgcctgat tcactacgtt
     9241 ggtcagcaga ttttccagct cattattcag cgtatacacc gtcagctcct gcttgcgaac
     9301 gaacggatga gtaatgctgc gccgcagcgc caggcgcaca tcggccgcca gcagaatatg
     9361 atctttagtc accgtgctac tggcgaccag cacggtggcg atggtgacga tatcgcgcag
     9421 ggaaacgcct tcggtcagca gcgcccggta cactttcagc aattggctgt aattgagcgc
     9481 cgcgcttaaa tcctccgcca ggcgcggtgc catcgacgac aaacggttat gcaactgcgt
     9541 gatgtcatca tagttaaaca aatcaggaat atagctgcgc acaatcttgt tcacatgggt
     9601 ggcaatcaca ctggcgctgt cgatcacctg ataccccata ttcagagcct tcgctttctg
     9661 cgcaggctgg atccaggtga ccggtatccc gtacgccggg tcgttcccca gcacgccgtc
     9721 aatctcgccg taggtttcgc tggagggcag cgccatcagt ttatccgccg gaatatccgc
     9781 ttcatcagcc ttaatgccgt tgatgaaaat agcgtactgg ctgggcttga ggcggaagtt
     9841 ttcccgaatg cggatctccg gcagcagcac gccgttaccg tcggaaatca cctgccgaac
     9901 gccgcgaatc cgctgggtga gcgggttgcc ctgcgctttg tccaccagcg ccaccagctt
     9961 gtagccgagg cttaagctga tgggctcgat cagcggaata gtttcccaac tgacctgttg
    10021 ttcgctggtt tcagtgatgg tgcgggtcag cgtttcgagg cttttctctt ccgcctccgc
    10081 cgcctgcggg cgtttgctca tccgccagcc ggtaaagcca agcagggcgc tgaacagcaa
    10141 aaacggcaga tgcggcatcc ccggcaccac cgccagcacg aacatgatcc cggtggcggt
    10201 gtagagcacc gacgggcttg ccagcaactg gtggcgcacg tcatgggcga tatcgccgtt
    10261 gtcgctgacg cgggtgacga taatcgccgc cgcggtggag agcagcaggg aagggatctg
    10321 cgccaccagg ccgtcgccga tggtcatcag cacgtattgc tggaaggcgg cgtcggcgct
    10381 caggttgtat ttgaagatgc cgatacagac gccgccgctc aggttgatcg ccagaatcat
    10441 catcccggcg atggcgtctc cgcgtacaaa cttcgatgcc ccgtccatcg cgccgtagaa
    10501 atcggcctcg ctggcaacat ctttacgccg ggtttgcgcc tgcgcctggt tgatcaatcc
    10561 ggcgttaaga tcggcgtcaa tcgccatctg tttgccgggc atcgcgtcca gggtaaagcg
    10621 ggcagaaacc tcggaaatac gctcggcctc tttggtgacg acgataaagt tgattgtcaa
    10681 cagcgtcagt aaatccgagc gtgaaagcat tatcgccgcg ctgcacgggc agtcgatttt
    10741 taacggtggt gggctgtcgc cgttaaataa aatcagcccg tctcatccgc caaaacctgc
    10801 caccgtggcg gtgccggaag agacggagaa aaaagcgcgt gatgtgaatg agaaaacggc
    10861 gctgctgaag aagaaatccg ccaccgagct tggcgagttg gcaaccagta tcaacaccat
    10921 tgcccgtgat gcgcatatgg aagcgaatct ggagatggag attgttcccc agggattacg
    10981 cgtgctgatt aaagacgacc agaatcgcaa tatgttcgaa cgcggcagcg cgcagattat
    11041 gccgttcttt aaaactttgc tggtggagct cgcgccagtg ttcgactcgc tctataacaa
    11101 aattattatt accgggcata ccgatgcgat ggcctacaaa aacaatatct acaacaactg
    11161 gaacctttcg ggcgatcgcg cgctttcagc tcgtcgggtg ctggaagagg ccggaatgcc
    11221 ggaagataaa gtgatgcagg taagcgcaat ggcggaccag atgctgctgg acgcgaagaa
    11281 tccgcaaagc gcgggcaatc ggcgtattga gattatggta ctgaccaaaa gtgcgtccga
    11341 tacgctgtat caatactttg gtcagcatgg ggataaagtg gtgcagccgc tggtgcaaaa
    11401 gctggataag cagcaggtgc tttcgcagcg aatgcgctaa atgctgaatc tttacgcatt
    11461 tctcaaaacc tgaaatcact gtatacttta ccagtgttga gaggtgagca atgcgtaaaa
    11521 tcattcatgt ggatatggac tgctttttcg cagcggtgga gatgcgcgac aatcccgctc
    11581 tgcgcgatat ccctattgct attggcggca gccgcgaacg tcggggggtg atcagcaccg
    11641 ccaattatcc cgcgcgtaaa tttggcgtac gtagcgctat gcctactggg atggcgctca
    11701 aattatgccc acatctcacc ttgcttccgg ggcgctttga cgcctacaaa gaagcctcaa
    11761 atcatatccg tgaaatcttc tcgcgctaca cctcgcgcat tgaaccgttg tcactggatg
    11821 aggcttatct cgatgtcacc gatagcgtcc attgccacgg ttctgcgacc ctcatcgccc
    11881 aggaaatccg ccagacgatc ttcaacgagc tgcaactgac ggcatctgcg ggcgtggcac
    11941 cagtaaagtt tctcgccaaa atcgcctccg acatgaataa acccaacggt cagtttgtga
    12001 ttacgccggc agaagttccg gcatttttac aaaccttacc actggaaaaa atccccggcg
    12061 tcggcaaagt ctcggcggca aaactggaag cgatggggct gcggacctgc ggtgatgtac
    12121 aaaagtgtga tctggtgatt ctgcttaaac gctttggcaa atttggccgc attttgtggg
    12181 agcgtagtca ggggattgac gagcgcgacg ttaacagcga acggttacga aaatccgtcg
    12241 gcgtggaacg cacgatggcg gaagatattc atcactggtc tgaatgtgaa gcgattatcg
    12301 agcggctgta tccggaactt gaacgccgtc tggcaaaggt aaaacctgat ttactgattg
    12361 ctcgccaggg ggtgaaatta aagttcgacg attttcagca gaccacccag gagcacgtct
    12421 ggccgcggct gaataaagct gacttaatcg ccaccgcgcg taaaacctgg gatgaacgcc
    12481 gcggcgggcg cggtgtgcgt ctggtggggc tgcatgtgac gttgcttgac ccgcaaatgg
    12541 aaagacaact ggtgctggga ttatgatgta tactattatg tatattctgg tgtgcattat
    12601 tatgagggta tcactgtatg catcggattc tcgctgaaaa atcggtcaat atcactgagt
    12661 tacgtaaaaa cccagctaaa tactttattg atcaaccagt tgcggttctt tctaataatc
    12721 gccccgcagg atatctctta agtgccagcg cattcgaagc gttaatggac atgcttgctg
    12781 aacaagagga gaaaaagccc ataaaggcgc gctttcgtcc aagtgctgca agattagagg
    12841 aaattacacg ccgcgctaaa caatatctta atgatatgac ggatgatgat ttcaatgact
    12901 ttaaggaata aggatgcggg tattcaaaac aaaacttatt cgcctgcaac ttacagcaga
    12961 ggaacttgat gcgttaacgg cggattttat ttcctataag cgtgacggtg ttttgccaga
    13021 tatatttggt cgcgatgcac tctacgacga ctcgtttacc tggccattaa tcaaatttga
    13081 gcgagttgct catattcatc tggcaaatgt gaataatcca tttccgccac agttgcgcca
    13141 attcagcaga acgaatgacg aagcgcattt ggtatattgt cagggtgcgt ttgatgagca
    13201 agcatggttg ctcattgcca ttctgaagcc tgaacctcat aaactggctc gagataacaa
    13261 ccaaatgcat aaaattggaa aaatggcaga agcgtttcgc at
//
LOCUS       DAJTYG010000099        13204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTYG010000099 DAJTYG010000000
VERSION     DAJTYG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.099"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..1145
                     /locus_tag="OO568_003167"
     CDS             <1..1145
                     /locus_tag="OO568_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642419.1"
                     /translation="NDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQTSIQMVTL
                     LNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSDNTCELTIN
                     EPLKNQEISTGNINVNCPLKDIYNEIRRLNVIFSCGTGDIVDLSSLDLRNVDLDYYDF
                     TDKHMANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKITPVLISISDMY
                     SEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELTKEIYKSSSKI
                     NNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYTMDLNIEDSSS
                     EELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(1653..2029)
                     /gene="rnpB"
                     /locus_tag="OO568_003168"
     ncRNA           complement(1653..2029)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO568_003168"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2062..3213)
                     /gene="garK"
                     /locus_tag="OO568_003169"
     CDS             complement(2062..3213)
                     /gene="garK"
                     /locus_tag="OO568_003169"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6642420.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGAGSLTDDVGV
                     VHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(3310..4200)
                     /gene="garR"
                     /locus_tag="OO568_003170"
     CDS             complement(3310..4200)
                     /gene="garR"
                     /locus_tag="OO568_003170"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6642421.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(4230..5000)
                     /gene="garL"
                     /locus_tag="OO568_003171"
     CDS             complement(4230..5000)
                     /gene="garL"
                     /locus_tag="OO568_003171"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR6642422.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(5016..6350)
                     /gene="garP"
                     /locus_tag="OO568_003172"
     CDS             complement(5016..6350)
                     /gene="garP"
                     /locus_tag="OO568_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6642423.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDSAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            6725..8296
                     /gene="garD"
                     /locus_tag="OO568_003173"
     CDS             6725..8296
                     /gene="garD"
                     /locus_tag="OO568_003173"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6642424.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPTSELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(8348..9172)
                     /gene="agaR"
                     /locus_tag="OO568_003174"
     CDS             complement(8348..9172)
                     /gene="agaR"
                     /locus_tag="OO568_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR6642425.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            9421..10221
                     /locus_tag="OO568_003175"
                     /pseudo
     CDS             9421..10221
                     /locus_tag="OO568_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
     gene            10221..10685
                     /gene="agaV"
                     /locus_tag="OO568_003176"
     CDS             10221..10685
                     /gene="agaV"
                     /locus_tag="OO568_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR6642426.1"
                     /translation="MVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNL
                     MEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVG
                     NMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10696..11475
                     /locus_tag="OO568_003177"
     CDS             10696..11475
                     /locus_tag="OO568_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR6642427.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGTEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11465..12274
                     /locus_tag="OO568_003178"
     CDS             11465..12274
                     /locus_tag="OO568_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR6642428.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            12361..12795
                     /locus_tag="OO568_003179"
     CDS             12361..12795
                     /locus_tag="OO568_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6642429.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEEYPVEEGI"
     gene            12792..>13204
                     /locus_tag="OO568_003180"
     CDS             12792..>13204
                     /locus_tag="OO568_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6642430.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         3339 a         3412 c         3231 g         3222 t
ORIGIN      
        1 gtaatgatat tagtagtgat ttagtccgtc gaaaaatgaa tgaccttcct gaaagcccca
       61 taggaaataa cctcgaagca ctagcacccg gtatagaaaa actaaaacag acctctattc
      121 aaatggtcac tttacttaac gcgttacaac caagtggaaa atgcattatc actggtgatt
      181 ttcaaaaaga attagcgtac ttacaaaatg taattcttta taatgactcg tctcttcgtc
      241 tggatttttt aggttataac gcccaaatta ttcaacgatc ggacaatacc tgcgaactta
      301 ccattaatga accgttaaaa aaccaggaaa tatctacagg caatatcaat gttaattgcc
      361 cattaaaaga tatttacaat gaaatcagga ggttgaacgt aatctttagt tgtgggactg
      421 gagatatagt tgatctatcc tctctggact tacgtaatgt cgatttagat tattatgatt
      481 tcacagataa acatatggct aatactattc taaatcattt taaattggat tcaacaaatt
      541 ttactaatgc caacatgttt caggttaatt ttgttagttc aacacaaaac gccacaatct
      601 cctgggatta tttactaaaa ataacgcctg ttttaataag cattagcgat atgtattctg
      661 aagaaaaaat caagtttgtc gaaagttgtt taaatgagct tggagacatt accgaagaac
      721 aattaaaaat tatgagattt gcaattataa aatctatacc aagggcaact cttacagata
      781 aattagaaaa tgaattaaca aaagaaatat ataaaagctc atcgaaaatc aacaattgct
      841 tgaacagaat taaattacca gagatgaaag aattctcatc aaaaaaaata tatgattaca
      901 tcgatacaat cattgaagat tatgaaaata tcaaagaaaa tgcttatctg gtcgtccccc
      961 aaattaatta tactatggat ttaaacatag aagactctag ctcagaagag ttaataccct
     1021 cgagaaagac gaaaattctc cggacaatgg ctttgaggtc ggggaatata acacatatga
     1081 agcatataac tcagagaatc aatattttac cagagaggac tatacgtata attacgacct
     1141 tttaaatgca atatagactt aataacttct tacaaagtct attattaata ccgtagatat
     1201 tcatcttacc ctgtgcacca aaagcaaaaa cttatcgacg cgggtttgaa actgatggca
     1261 accgcaaaaa tgcctgatgc gctacgctta tcaggcctac gccatctctg caatatattg
     1321 aatttgcgtg cttttgtagg caggataagg cgttcacgca gcatccggca tgaataaagc
     1381 gcactcaaca atctgaaacc cgccggaacg gtttattacg tacatcaggt gaaactgacc
     1441 gataagccgc tttcttttgg gtatagtgtc gtggacagtc attcatcttt ctgcccctcc
     1501 aaaagcaaaa acccgccgaa gcgggttttt acgtatatca ggtgaaactg accgataagc
     1561 cgctttcttt tgggtatagc gtcgtggaca gtcattcatc tttctgcccc tccaaaagca
     1621 aaaacccgcc gaagcgggtt tttacgtaaa acaggtgaaa ctgaccgata agccgggttc
     1681 tgtcgtggac agtcattcat ctaggccagc aatcgctcac tggctcaagc agcctacccg
     1741 ggttcagtac gggccgtacc ttatgaaccc ctatttggcc ttgctccggg tggagtttac
     1801 cgtgccacgg actgttacca gccgcgcggt gcgctcttac cgcacccttt cacccttacc
     1861 tgatcccgct tgcacgggcc atcggcggtt tgctctctgt tgcactggtc gtgggtttcc
     1921 cccccaggcg ttacctggca ccctgcccta tggagcccgg actttcctcc cctccgcccg
     1981 tctcccccga agaggacgac gacgaagcgg cgactgtctg gtcagcttcg gcgcgcagta
     2041 tagagggttt gcgcgccctt gtcaccccgt gttgcgcatt ccaatcgcca gtgtcgcggc
     2101 aatattacgt gaagcacggc agatattgtc ataagccccg cggaatgctt cgtccaacgt
     2161 acctatgctg gtcaatacgc tgaagaccgc atcaatgcca tgctgatgta caacgccaac
     2221 atcatcggtc aggctacccg cacccgcaat gccaatcacc ggtttatggt acttcttcgc
     2281 cacgtttgcg acgccaatcg gtaccttgcc gtgaatactc tggctgtcaa tacgcccttc
     2341 accggtgatc accagcgtac aatcgtgaat atgttcctcc agattcagcg ccgtagtgac
     2401 gatttcaata ccacttttca gttccgcacc aagaaacgcc atcagcgccg cacccatacc
     2461 gcccgcagct cctgcgccgg ggacatcttt tacatcaaca tgcagcgctt ttttaatgac
     2521 atcggcatag tgagagaggt tattgtccag ctcaacaatc atcgcttcac tggctccctt
     2581 ttgtgggcca aagatgcgcg atgcgccgtt atcgcccacc agcggattgg tgacatcaca
     2641 agcgacgcga atgacgcaat cttttaagcg cggatcgagg ccggaaatat caatatcatt
     2701 cagagtatta agactaccgc cgccaaaacc aatttcattg ccgttggcgt cgcataattt
     2761 cgcccccagc gcctgtacca tgcctgcgcc gccatcattt gtagcgctac cgccaatgcc
     2821 gataataatg tttgtcgcgc cgctctccag cgcctgcagg attaactcgc ctgtgccgcg
     2881 tgaagtggtc acgagtggat cgcgtttttc cgcaggtacc agctccagcc cactggccgc
     2941 cgccatttca ataaacgcgg ttttgccatc gccggagatc ccccaactgg cattcacttt
     3001 ctcgcccagc ggccctgtaa cccaggcgtg acgttcagaa ccctgggtgg ctgcaatcat
     3061 cgcttccacc gttccttcgc caccgtcggc aaccggaata gaaacgtact gtgcatcagg
     3121 aaaaatttcc cgaaatcctt tttctatcgc ctgcgcaacc tcgctggcag ataaactttc
     3181 tttataagag tctggggcga ttacgatttt catacctatg cctgttacca catgacgccg
     3241 gagggcgttt ctcttattcg gcctggattc caggcccgga ttgcaatacg ccatccgggc
     3301 acgacgtcat taacgagtaa cttcgacttt cgccagtttt tcgtagtagc acgccagggc
     3361 gctatgatcc gccgttccta aaccatctgc tcgcagtgcc tgcatcatct ccataaccgc
     3421 agctgtgagc ggcagttgcg cgccgacgcc gtgggaagta tccagcgcat ttgccagatc
     3481 cttaatatgc agatcaatac ggaagcccgg cttgaagttg cggtccatca ccatcggcgc
     3541 tttggcatcc agcacggtac tgcccgccag tccaccgcga attgcctgat acaccaggtc
     3601 cgggttaacg cccgctttag ttgccagcgt taacgcttct gacatcgcgg caatattcag
     3661 cgccacaatg acctgattcg ccagtttggt gacgttacct gcaccaattg ccccggtatg
     3721 caccacggaa cccgccattg ctttcatcaa atcatagtat ttgtcgaaaa tagccttgtc
     3781 gccgcccacc atcactgaca gcgtaccgtc gatggctttc ggttcaccgc cgctcaccgg
     3841 agcatccagc atatcaatgc ctttcgcttt cagcgcttcg ctgatttcac ggcttgccag
     3901 cggtgcgata gaactcatat cgatcaatac cgtacctggc ttcgcgcctt caataatgcc
     3961 attctcaccc agcgccacct ctttcacatg aggggagttt ggcagcatgg ttatgatgac
     4021 gtcgcactgt tcagcgatcg ctttagccgt agaggctgtt tctgcacctg cagcaatcac
     4081 gtcagcaata gcttctgggt tacggtcagc aaccaccagc gagtaacctg ctttcagaag
     4141 gtttttactc attggtttac ccataatccc caggccaata aaaccaactt tcatagtcat
     4201 atcaatcatc tctcttgttg cggtggtggt tattttttaa aggtatcagc cagtttatga
     4261 gtggcagagc ggaagacgcc gagatcgctg ccgacagcca caaacgtcgc gccccattcc
     4321 agataacgac gcgcatcggc ttcgaccggc gcgaggatac cgctgggttt gccgtgcgcg
     4381 ctggcacggt taaaaatgtg ctgaattgct ttttgtacat ccgggtgtga tgcattgccg
     4441 agatggccta atgccgcggc cagatcgctg gggccgacga agatgccgtc tacgccttcg
     4501 gtagcggcaa tggcatcgac gttatctacg ccctgctgac tttctatctg gaccagaata
     4561 gtgatgttct tgttcgactg agcgaaataa tccgccacgg tgccaaacat attggcgcgg
     4621 tgagaaacgg agacgccgcg aatgccttcc ggtgggtaac gggttgatgc caccgcctgc
     4681 tctgcttcct cttttgtttc tacaaaagga atcaggaagt tatagaaacc gatatccaga
     4741 agacgcttaa taattaccgg ctcgttggtc ggcactcgca ctactggcgc gctggcgctg
     4801 cctttcaagg ccattaactg cggaataaac gtggagatat cgtttggcgc atgttcgcca
     4861 tccagcacca gccagtcaaa cccagccaaa ccaagaactt cagtgctaat cgggttagag
     4921 agtgctgacc agcaaccaat ttgtacctgt ttcgcagcca gtgcggcttt gaatttattc
     4981 gggaaaacat cgttattcat cgcttatacc tttgcttatt tctgcaattc catacgttta
     5041 atgtcgccaa ctacgaagag gtagcagacc atcgccatca gcgctgaaca tcccacgaaa
     5101 accagtgctg cattgaagga gtgcagttca cttaccaggt agccaatcac cagtggagtg
     5161 acaatggagg caacattgcc aaagacgtta aagacgccgc cacagaggcc aacaatctct
     5221 ttcggcgcgc tgtcagaaat caccggccag cccagagcac caaatccttt gccaaaaaaa
     5281 gccagcgcca tcagcatgac caccagcgtg gtgttgttgg tgtagttaca taagatgatg
     5341 gtggaagcca gcaacattcc cagcacaatc ggtagcttac gtgccagggt cagggataaa
     5401 ccgcgtttga tcagataatc cgagaagaca cctcccagca cgccgcccgc aaaaccacac
     5461 agtgccggaa tcgaggcgac cagacccact ttcagaatcg acatgccttt ttcctgcacc
     5521 agataaatcg ggaaccaggt gaggaagaac caggtgatgg tgttgataaa atattgtccg
     5581 aaaaatacgc ccaacatcat gcggttagag agcaattgct tgatgtaatg cagtttggga
     5641 ccgcttgctg ccgcgctgcc cggctttttg tggtccatat cgaccaccgc gccattttca
     5701 gagataaact tcagctcttc ctcagacata cgtgggtgat ctgtcgggtt atgaatcaac
     5761 ttgatccaca gcgccgtcag cacaaaacca atcaccccca taacggtaaa gacgtgctcc
     5821 catccccagg cgaaagtcag ccagccaagc agaggcgaaa agagcgccag cgagaaatat
     5881 tgtgccgagt taaagatggc ggaggcagta ccacgttctt tcgtcgggaa ccaggcggcg
     5941 acaatacggg cgttcgccgg gaatgatgga gcttccgaga atcccagcat aaagcgcata
     6001 aagaacatgg agatccctgc ccaggccagc gggaacatat caacaaagcc ttgcaggaag
     6061 gtgaacagcg accagaaaaa gaggctgtag gtgtaaactt ttttcgagcc aaacttatca
     6121 agcaaccagc cgccggggat ttgcatcagc aagtaggccc agccaaaagc ggagaagatg
     6181 taacccatcg aaaccgcact taactgcaac tcttttgcca cttcggtacc agcaatagac
     6241 agcgttgcac gatcggcgta gttaacggcg gtaacaataa aaataatcag taatattaaa
     6301 tagcgggtat gcacaccttt ccttttttcg tcaacggtgt ccagaatcat tttatttacc
     6361 tcgggtactt atgctgattt ttattattat ggggaaggtg ttatttataa gtttcattta
     6421 tgccgtaacg acaatgaact cgggaattag tataagcagc gcgagaataa taatcattgt
     6481 gcaaatgcta atttaattaa tactttttaa atattatttt gagcatatgc acataaggtt
     6541 gcgcgctaaa gcacagattt gcgctttacc ttgccgggcg gcactgcaat ccctgaaatg
     6601 attgacattg atcacatttc tgcgtttaaa ctcctgacat tcttatttca cccaatgaag
     6661 tcatttattt ttaaatgaga ccaggtcctc attttaataa cccctggctg gagaatattg
     6721 cacaatggcc aacatcgaaa tcagacaaga aacgccaact gcgttttata taaaagttca
     6781 cgacacagat aatgtggcaa ttattgttaa tgataatggc ctgaaagcag gaacgcgttt
     6841 tccggatggg ctggaattaa ttgaacatat tccccagggg cataaagtcg cattgctgga
     6901 cattccggct aatggtgaaa ttattcgtta tggcgaagtg attggttacg ccgtgcgtgc
     6961 aatcccacgc ggaagctgga tcgacgaatc aatggttgta ctaccggaag cgccgccgtt
     7021 acacacgctg ccactggcaa ccaaagtccc ggaaccctta ccgccgctgg aaggatacac
     7081 ctttgagggc tatcgcaatg ccgatggcag cgtgggcacc aaaaacctgc tcggcatcac
     7141 caccagcgtc cactgtgtgg caggcgtggt ggactatgta gtaaaaatca ttgaacgcga
     7201 tctgctaccg aaatacccga acgtcgatgg cgtggtgggg ctgaatcatt tgtacggttg
     7261 tggcgtggcg attaacgcac cggcggcagt tgtacctatt cgtaccattc acaatatttc
     7321 gctgaaccct aactttggcg gcgaagtaat ggtgattggc ctgggttgtg aaaagttgca
     7381 gcctgagcgc ctgctgactg gaacggatga tgtgcaagct attccagtag aaagcgccag
     7441 cattgtcagt ttgcaggatg aaaagcatgt cggttttcag tccatggtcg aggatatttt
     7501 gcaagtcgcc gaacgccatc tacaaaaact gaatcaacgt cagcgagaaa cctgtccgac
     7561 ttcagaactg gttgtcggca tgcagtgcgg tggcagcgat gcgttttctg gcgtaacggc
     7621 aaacccggcg gttggctatg cgtctgatct actggtgcgc tgcggcgcaa cggtgatgtt
     7681 ttcagaagtc acggaagtgc gtgacgcgat ccatctgctg acaccacgcg cagtgaacga
     7741 ggaggtcggc aaacggctgc tggaggagat ggagtggtac gataactatc tcaatatggg
     7801 aaaaaccgac cgcagcgcca acccttcgcc gggcaacaag aaaggcggtc tggcaaacgt
     7861 ggtagagaaa gcgctcggct ccattgctaa atcgggcaaa agtgcaattg ttgaagtgct
     7921 gtcgcccggt caacgcccga ctaaacgcgg attaatttac gccgcgacgc cagccagcga
     7981 ttttgtctgt ggcacgcaac aggtggcttc gggtatcacc gtgcaagtgt ttacgaccgg
     8041 tcgtggtacg ccgtacggcc tgatggcggt acccgtcatt aaaatggcaa cccgcaccga
     8101 gctggcgaac cgctggtttg atttaatgga tattaatgcg ggcaccatcg ctaccggcga
     8161 agaaactatt gaagaggtgg gctggaagtt gttccacttt attctcgacg tcgccagcgg
     8221 gaagaagaaa accttctcgg atcaatgggg actgcataac cagctggcgg tgtttaaccc
     8281 agcaccggtg acctgataat tcaattcgta ttatgcccga tagcacacgt ttatcgggca
     8341 tattgagtca cagcgaagac gcgtgatccc cgaccagaat cacttcaacc ccagcctttc
     8401 gcagtccttc cagactatcc gcaggaatgc cttcatcaac aatgatcatg tcgatacgtt
     8461 gagtatcaat gatcttatgt aaactcgaac gattgaattt actggaatcg gtgaccacga
     8521 tgatccgttc cgcaacttcg cacatccgac ggtttaaacg ggcttcatct tcattatgtg
     8581 tgctgacgcc gcgctccaga tcgatcgcat ctacaccaag aaacagcata tcgaagtggt
     8641 aattttgcag cgattgctca gcctgatcgc cgtaaaaaga ttgcgactga cggcgcaaat
     8701 gcccgccggt catcagcagc tcaacgcctt ccgcttccag caacgcatta gccacgttca
     8761 taccgttggt catcgcaatt acgtcagtgt acttgcgcat cagacgagca atctcaaaag
     8821 tggtggtccc ggaatcgagg atcacccgat gacctggctg aatcaactca acggcagctt
     8881 tcgcaacgct gcgtttcatc gcggtgttca gtgcgctttt atcttccact gatggctcga
     8941 ctgacggcgt cgtgctatcg cagatcaacg cgccaccata ggcacgcaca gcgatcccct
     9001 gcttttccag aaaagccaga tcattgcgga tcgtcacagt agatacgcca tacaatgccg
     9061 acagatcgtt aacctgcaca ctcccttgct gtcgcagacg ctgaatgatc tgttctcgtc
     9121 gctcgctggt gcctgtcact cgcttctcac ctgaagcgtc ggtattactc atagtaagtc
     9181 ctttcgtaaa actttcgttt catttcgttt tgcctattaa cgcctttcta ttaagcaaat
     9241 gcaagcccat cttgcccatt ggcgcaagct actctcgttt cagtgacttt cattatgttt
     9301 cttttgtgaa tcagatcaga aaactattat atttcgtttt atttttatct caccatgatg
     9361 cagtatcaat tgaaacaaaa cgaaagatta atatcgcagt aatctgaact ggagaggaaa
     9421 gtgaaacatc tgacagaaat ggtgagacag cacaaagcgg gcaaaacaaa tgcaatttat
     9481 gccgtttgtt ccgcacatcc gctggtgctg gaggctgcaa tccgctacgc cagtgcaaac
     9541 caaacgccgt tactgattga agcaacctcc aatcaggtag accagttcgg cggttatacc
     9601 ggaatgacgc ccgccgattt tcgcggcttt gtttgtcagc tcgccgactc gttgaatttc
     9661 ccgcaggatg cgctgattct gggtggtgac catctggggc caaaccgctg gcaaaacctg
     9721 ccagccgctc aggcaatggc caatgccgat gatttgatta aaagctacgt tgcggcagga
     9781 ttcaaaaaaa tccaccttga ttgcagcatg tcctgtcagg acgatccaat tcccttaact
     9841 gatgacatcg tggctgaacg cgccgcccgt ctggcgaaag tggcggaaga aacctgtcgc
     9901 gaacactttg gcgaagccga tctggagtat gtcattggta ccgaagtgcc ggtacctggc
     9961 ggcgcgcatg aaaccttaag cgagctggcg gtcaccacgc cggatgccgc ccgcgccacg
    10021 ctggaagccc atcgtcacgc ctttgaaaag caaggtttga atgccatctg gccacgcatc
    10081 attgccctgg tggttcaacc cggcgtcgaa ttcgatcaca ccaacgttat tgattatcag
    10141 cccgccaaag cgagcgcctt aagccagatg gtcgaaaact acgaaacgct gattttcgaa
    10201 gcgcactcta ccgattatca attgttttaa gccggattga tgaacgcttg attcacggtc
    10261 aggtcggcgt tcagtgggtc ggatttgcgg gggcaaatct ggtgctggta gccaacgatg
    10321 aggttgccga agatccggta caacaaaacc tgatggaaat ggtactggca gaagggatcg
    10381 ccgtacgttt ctggacgctg caaaaagtta tcgacaacat tcatcgcgcc gccgatcgac
    10441 agaaaatcct gctggtttgt aaaacacccg ccgatttcct gacgctggtg aaaggtggcg
    10501 ttccggtgaa tcgcattaac gttggcaata tgcactacgc caatggcaaa caacaaatcg
    10561 ccaaaacggt ttctgtggat gcgggcgata tcgcagcatt taacgacctg aaagccgctg
    10621 gggtggaatg cttcgttcag ggcgtcccga cagagcctgc tgtggacctc tttaaattac
    10681 tttgagggat tcatcatgga aatcagcctg ttgcaggcat ttgcgttggg cattatcgcc
    10741 tttatcgctg gcctagatat gtttaacggc ttaacccata tgcaccgccc ggtggtcctc
    10801 ggcccgttgg tcgggctggt acttggcgat ctgcataccg gaattttaac cggcggtacg
    10861 ctggaactag tgtggatggg gctggccccg ctggcgggcg cacagccgcc taacgtgatt
    10921 atcggtacta tcgtcggcac ggcgtttgcc attactactg gcgtgaaacc cgatgtcgcg
    10981 gtcggtgtcg ccgtaccttt cgctgtcgca gtacagatgg ggattacctt cctgttctcg
    11041 gtgatgtctg gcgtgatgtc tcgctgcgac cggatggcgg agaacgccga tactcgcggc
    11101 attgaacgcg tgaactatct ggcactgctg gcactcggca ctttctattt tctctgcgct
    11161 ttcctgccta tctacttcgg cacggaacat gcaaaaacca tcattgatgt cctgccgcaa
    11221 cgattaatcg acggcctcgg cgtcgcgggc ggcatcatgc cagcaatcgg ttttgccgtg
    11281 ctgctgaaaa tcatgatgaa aaacgtctac ataccctact tcatcctggg ctttgttgcc
    11341 gcagcctggc tcaagttacc ggtgctggct attgctgccg ccgcgctggc gatggcgctg
    11401 atcgatctgc tgcgtaaatc tcctgaaccg actcaacctg cggcacagaa agaggaattt
    11461 gaagatggca tctaatcaaa ccaccctgcc gaacgtcagc gaaaatgaag aaacactgct
    11521 gactggcgtc aatgaaaacg tgtatgaaga tcggagcatt gccgcggagc tgacgaaaaa
    11581 agatatcaac cgcgtcgcct ggcgttccat gctgttacaa gcgtctttta actacgaacg
    11641 tatgcaggct tctggctggt tgtacggtct gctgcctgct ctgaaaaaga tccacactaa
    11701 taaacgcgac ctggcgcgcg ccatgaaggg gcatatgggt ttcttcaata cccatccgtt
    11761 tctggtgacg tttgttatcg gcattatcct tgcgatggag cgttctaagc aggacgttaa
    11821 cagtattcag agcaccaaaa ttgccgtcgg tgcgccgctt ggcgggattg gcgatgcgat
    11881 gttctggcta acgctactgc cgatttgtgg cgggataggc gcaagcctcg cgctacaagg
    11941 ctctattctt ggcgcagtcg tctttattgt gctgttcaac gtggtacact tggggctgcg
    12001 ttttggtctg gcgcattatg cttaccgcat gggcgtggcg gcgattcccc ttattaaagc
    12061 gaataccaaa aaagtcggcc atgctgcgtc aatcgttgga atgacggtaa tcggcgcact
    12121 ggtggcaacc tatgttcgtt taagcaccac gctggaaatc acggcgggcg acgcagtggt
    12181 taagttacag gctgatgtta tcgacaaact gatgcctgcc ttcttaccgc tggtctacac
    12241 cctgaccatg ttctggctgg tacgccgcgg ctagagtccg ctgcgcctga ttgcggttac
    12301 cgtggttcta gggatcgtcg gtaaattctg ccatttcctt taaatacaat gaggtattag
    12361 atgttaagta ttattttgac agggcatggc gggtttgcca gtggcatgga aaaagcaatg
    12421 aagcaaatcc tcggcgatca atcgcagttt attgccatcg attttcccga aacctcaagc
    12481 gccgcgctgc ttacatcgca gcttgaagag gccatcgccc aactggattg tgaagacggc
    12541 attgtttttc tgacggattt gctgggcggc acgccgtttc gcgtagcttc aacgctggcg
    12601 atgcaaaaac cgggctgtga agtgattacc ggcaccaatt tgcaactgct actggagatg
    12661 gtgctggagc gcgaagggtt aagcggtgaa gaattccgtg tacaggcgct ggagtgtgga
    12721 caccgagggc tgaccagtct ggtggacgag ttaggccgct gccatgaaga atatccggtc
    12781 gaggaaggaa tatgacacac gttctgcgcg ccagaaggct gctgactgaa gagggatggt
    12841 tcgatgacca tcagttgcgt gttgctgacg gtgtcatcgc agcaatcgaa ccgattccag
    12901 tgggcgtgac tgaacgcgat gcggaactgc tctgccccgc ttacatcgac acccatgtac
    12961 acggtggtgc gggcgttgat gtaatggatg acgcgccgga tgtactcgac aagctggcaa
    13021 tgcacaaggc acgcgaaggt gtcggcagtt ggttaccgac caccgtaacc gcgccgctta
    13081 ataccattca tgcggcgctg aaacgtattg ctcaacgttg ccaacgcggc ggacctggtg
    13141 cgcaagtgct ggggagttat ctcgaaggac cgtacttcac gccgcagaat aaaggcgcgc
    13201 atcc
//
LOCUS       DAJTYG010000100        12775 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTYG010000100 DAJTYG010000000
VERSION     DAJTYG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.100"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(68..2017)
                     /locus_tag="OO568_003181"
     CDS             complement(68..2017)
                     /locus_tag="OO568_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6642431.1"
                     /translation="MTDSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDATAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2102..2438)
                     /locus_tag="OO568_003182"
                     /pseudo
     CDS             complement(2102..2438)
                     /locus_tag="OO568_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
     gene            complement(2438..2794)
                     /locus_tag="OO568_003183"
     CDS             complement(2438..2794)
                     /locus_tag="OO568_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6642432.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2794..4290)
                     /locus_tag="OO568_003184"
     CDS             complement(2794..4290)
                     /locus_tag="OO568_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6642433.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4287..4448)
                     /locus_tag="OO568_003185"
     CDS             complement(4287..4448)
                     /locus_tag="OO568_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6642434.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4458..5020)
                     /locus_tag="OO568_003186"
                     /pseudo
     CDS             complement(4458..5020)
                     /locus_tag="OO568_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5017..5541)
                     /locus_tag="OO568_003187"
     CDS             complement(5017..5541)
                     /locus_tag="OO568_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642435.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRYARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERQRHWTQELLSS
                     ELQRSLRPVKRKHK"
     gene            complement(5507..5923)
                     /locus_tag="OO568_003188"
     CDS             complement(5507..5923)
                     /locus_tag="OO568_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6642436.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            complement(5920..6243)
                     /locus_tag="OO568_003189"
     CDS             complement(5920..6243)
                     /locus_tag="OO568_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR6642437.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            complement(6288..8018)
                     /locus_tag="OO568_003190"
     CDS             complement(6288..8018)
                     /locus_tag="OO568_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR6642438.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGHGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNGEVETLNGK
                     LTELENLKSDLEAELAEVKRPAGGTQNKVAGEHKEAFIGFMRKGREDGLRELERKALQ
                     VGNDEDGGYAIPEELDRTILTLLKDEVVMRQEATVITLGGSDYKKLVNLGGTTSGWVG
                     ETEARQETATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEDWINSELALEFAEQEE
                     IAFTSGDGSKKPKGFLAYESTDEDDKTRAFGKLQHIASGAASGVTADAIIKLIYTLRK
                     AHRSGAKFMMNNSSLFAIRLLKDNDGNYLWRPGIELGQPSSLAGYGIVENEQMPDIAA
                     DAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQAIKLMKI
                     GAATRQKAAA"
     gene            complement(7996..9237)
                     /locus_tag="OO568_003191"
     CDS             complement(7996..9237)
                     /locus_tag="OO568_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6642439.1"
                     /translation="MFFQGLFQRKNNTPVTTPGMLAEELGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERMKRDFEERHLGLGNAHRPMILEMGLDWKPVALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(9237..9419)
                     /locus_tag="OO568_003192"
     CDS             complement(9237..9419)
                     /locus_tag="OO568_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642440.1"
                     /translation="MFLIILTPLAGVLGALLLSYGTWLIYPPAGFVVAGALCLCWSWL
                     VARYLDRGHRVASGGE"
     gene            complement(9431..11188)
                     /locus_tag="OO568_003193"
     CDS             complement(9431..11188)
                     /locus_tag="OO568_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6642441.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEEWGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(11188..11670)
                     /locus_tag="OO568_003194"
     CDS             complement(11188..11670)
                     /locus_tag="OO568_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6642442.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDAKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRVKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(11818..12168)
                     /locus_tag="OO568_003195"
     CDS             complement(11818..12168)
                     /locus_tag="OO568_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6642443.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(12211..12675)
                     /locus_tag="OO568_003196"
     CDS             complement(12211..12675)
                     /locus_tag="OO568_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6642444.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(12672..>12775)
                     /locus_tag="OO568_003197"
     CDS             complement(12672..>12775)
                     /locus_tag="OO568_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6642445.1"
                     /translation="DQLRRWTYAGGKQWKGLMTRREIEREVCLWGQQ"
BASE COUNT         2883 a         3789 c         2958 g         3145 t
ORIGIN      
        1 aaaacaaact ccattgttag catgttacgc gataattttg cctcatcttt caccacaaaa
       61 aaagaactca ccttttaaat ggagaatacc caacatcatg ggtgattttc ataaagggat
      121 cgcctgtttt cggcaaatcg ataacgcgca ttccaggcgg cgcgttgtca aacgtcacct
      181 taagttcgct gcgggttgcc ggtgaagatg caaggcttaa cagctgattc ctttccagtg
      241 atacattcgg ggtgtagcgt ttatcgggag ataaataccc atcaccacgt ggcatctgcc
      301 atccggtcat gtcatagaca aaatcatgga actgtttgcc ccactcgtcg aacttatcat
      361 tcagaccaaa tccactgttc agtgcatcag ccacaaaatt tttaacaagc cagggatgtt
      421 ctttttcaaa ctcctgcgcc atcatgccca gttcaagcaa tccaccaatc agactgatct
      481 ttccgaggcc ctttaatttc agccctgttt ttcctgccgc cgttgtccat tgattctggg
      541 cgactgttgc cgcacgaata cttgccaccg ccttcacacc aatatacagg gcagaaaccg
      601 ttgctattgt ttttactgcc tgttcccatc cccccattgc tctgacaacc tgatcaacct
      661 cctgccagac ttttttcaca accggaccga ccgtttccca gttatcaatt atgagatatg
      721 caccaccgac gagaagcgca attaatccct ttgccggcgt catattcatc acgctaccca
      781 tgatttttgt tatgcgtgtc aatgtgccaa tcgccacgcc catcgtcagt aatgccgcac
      841 cagttttcgc gatggttttt acgacctcag gattttctct gacaaacgtg cgcacttctt
      901 ccagaaaagg cttcatctct tttatgcctt cattgagtga aggcaggaag gtttccccca
      961 gcgtggaaga aatcgcattg atctggttct gcagcagcaa caactgattt tctgtcgtcg
     1021 cggcacgggc ggcatattct ttctgcattg agccgccata ctgctgcgca tccgccacac
     1081 gattaaagtt ggttcgcaac aaatccagat tcgtgagcag cggcgcaatg gcccccaggg
     1141 attccttccc gaacagcgct ttcagcacag cagcctgttt ttctttcggc acttttgcca
     1201 gagaatccag tacgtgcagc atggccccgc gggcgtcttt ctgcatatcc gccgccagtt
     1261 ttttcgggct aatgcgcaac gagcggagca cttttttctg cgatttggtg gcagaatccc
     1321 tcgctgtcag cgacaacata aaattcttta tccccgtcgc agcaatttct gactccaccc
     1381 ccattccggc aatggttgcc cccattgcgg caatctctcc ggaggccaca cccgcaacac
     1441 tgcctaaagg gccaatacgg gtcacaacat cagaaatctt tttcgcactt gcaggaccgg
     1501 tattgccaag atagttaatc ttatccgcaa gccctgccac ctctccctgt gtcagtttaa
     1561 aggcagtgcg ccactgcgcc atcatctgac cggattcttc tgccgtggcg tcaaaggcca
     1621 cgcccatctt cacggcatcg tcagtaaact gcatcagctc gtcacgggcg ataccagcct
     1681 gaccacccgc cgccacaatt tcggcgatac cttccgccga catgggcagt tctgttgaca
     1741 ggtcgcgcac ctgctccgtc attgccttaa acgcttccgg cgtatccaga ccatccacca
     1801 ctttccggac atccgccatt tttgattcaa gggcaatggc agatttgacc gggagcgcca
     1861 gcgcccccag tactgcggtt ccggcacccg ctgcgcccag tgacaggctg gcaaactcct
     1921 ttttaaaacc tttcagctgg cgctgcattc ccttcagcgg tgccgatacc ttatccacgg
     1981 cagtgatgat cgccttcagc tgaaaactgt cagtcatgct tcatctcctc gttaatgcgg
     2041 acggcctcgg cttccagttc tgtaaactgc gaaacagcca cccgtcgcag ctccagtgga
     2101 ttcagtttcc agaaccaggc aacattgtag agtcgtttcc cggaggtgct tcccgtctcc
     2161 gactgggtaa aaaaacgcag gatctgcatg ctggtcttaa aaatatccag ttttgccatc
     2221 tgcgccgccg atgaacgcgg gatccccgcc agcaacggga tatatttcag tgccacctgg
     2281 ctgtccagtt taatactgcc ttcaccggaa ataatgaaag gaaaacccag cgcctcgatt
     2341 tcgtcatacg tgggctcgcg caactccagc acatgcagca tttcgttatg cgctgtcacc
     2401 ggttttttta aaactatttc tgtaacgttc attgccatta ctgataccct ccctcttcgc
     2461 cgtggaattc aagatctgcc gtgccttctt cggcattatg attagcctct ccgtgcagcc
     2521 atgccgccga aagcacatac accatgccgt ttgccagctc agcggtaatc gtcatctggt
     2581 ctgaggtggt aattttgtcg accggaaagt ttttgggcac tttaaacgtg cccttaacgt
     2641 aaggtgaccg ccaggtctcc ttgtaatcca catcccctgc catgccgacg atgtcatccc
     2701 ggacattggt gttcatcggc acttcaatgc cacctgtcag cgataactgc tgaccatcca
     2761 ctttaaaaaa acaggttccg gcgatctttg ccattatgct gactcctctg aatactgaag
     2821 acggaactgg ttaaccaccg caaagacacg taactgatta acataatccg gcgggaacag
     2881 cgtattcagt cggttcgggt tatctgcatc acgctcaact atcagatact gtttaaacag
     2941 atcgtaattt tccacaatac cggcacgctc catctgacga tatgtcgcca gaagttcccc
     3001 tttgataacg gcaggagtga caatcgcctg ccccggacca aaacgggtac catcatttgc
     3061 cagcttgtga cgtccgtact tgcttgtgat gaccgatttc agtttgcgaa gaacatatgc
     3121 actggtatgc agagtttcac tgtccagata gctgttgtct gccacgccat acgcattctt
     3181 tttgtaggtg gttacggaac gctggatctg caacgtgccg ccttccacat aagccgtcgc
     3241 cacgccgtga gataaaaggg tctgctgctc tgtcatgatg aatcgcttac ctttcggtgc
     3301 cggaagcatc cccaccagct cccccgtctg tgtcggacgg gcaggatcat tccggataaa
     3361 taccgcttca cgggcaaggc gactggcaac cagttcatcg acaggcgact gggtttcttt
     3421 ttcgtaaccg gcaagcgtga tatgttgctg attatgcata tctccggcac caaccagctc
     3481 tgacagcgtt cccagttttg cggtatagac atgcccgtat aactggcgcg cataactcca
     3541 gcgaccgctg ctgtcattca tttcggtcat catcatattg atggaggcgg catcgttgaa
     3601 cggcagaccg ataaagtcga atgcctcatc gcccatagca gcaacagcgg cggtaagatc
     3661 aggcgctccg ctaccggcag caccgacttc cgtcaccacc cgaagccctg ctggcagaat
     3721 ctcaccacca ccagaaccat aataattcag acagaccggt aactcattac catacagccc
     3781 cttatgacgg gcagtcagcg tcaccacgcc tgcatcagat gacgccgtaa acggcagggt
     3841 gataaccccg tttaccgctt ccttaatcgc ggtggcaacc gcagtggcat catcgccatt
     3901 caccacaggc acctgtacac gggagcgccc gacatacaga ctcagggtgc cgctttcctc
     3961 tgcttctccg gcaaccgtca ccctgaccgt cgccgccgcc cctctggctt ccggtactgc
     4021 aataacatac agttcaccga aaggatctgt ctgacggtag acttccacca tacgcgccag
     4081 ctggctcccc gccccacaaa tctggcgggc ataatctgcc gatggcatca gcaccaggct
     4141 gttaacctca atggctgcat cgttgctggc atgcccgatc agtaatgcag gcgcgctggt
     4201 caccgctgta tttgccgcag aattgttcat ctcggcgtag aacaacggca ccagcgtatt
     4261 cgacggaaca gtattaaaac ttatcgtcat gattttttag ccttatcctg tttaacacgt
     4321 accacatcac ccgccgctat acggcggaac cagtagctgc tttcttccac atttcgccct
     4381 tcggaaggca aaaggtctcc acgggcaggg tcaggaaccg accgcccttt caggggtttc
     4441 acaaacatga gggactctta attctgaggg aaacgcattt ccagatggtg ctcaatgtca
     4501 ccatccgggc cagtaccagg atcgatgtaa tccacatcaa tactcagcag ggataaatcc
     4561 ggcaggcatt cacgtcctct gcctgtcgcg tgtcttcttc cgtgatttca tacttcgccg
     4621 taaaatcaaa ctggtaatac agttcgtaac ggttcagatc cagcagggtg ccaccgacat
     4681 aaacaatctc gcccccttgc gggtccggca tccatccaag cagggctttc cagagttctc
     4741 tccggacatc atgaacggcg tcataggctg cccactgccc tttttcatca cgctcattgc
     4801 tgagcaccac aatcacggag aagccttcag tcaaatcctg ccagtaatcg gtctgtgatt
     4861 tttgttcccc cggcgcatcg tcagagggaa ccacataggc agcaggcagg cgaagttttc
     4921 cggcatcagg gatcgcctta aactgcgccg cgccaccaac acgctcctca aaacgcgggc
     4981 atcgctcacg cagcgccgca attatcgttg tcagtttcat ttatgcttcc tttttaccgg
     5041 acgtaacgaa cgctgcagct cactggacaa cagttcctgc gtccagtgac gctgacgctc
     5101 aataacgtca gccataaagt tattacgcgg ggccagccgg aaagtcgaag aatggtgctt
     5161 cttctgccgc ttatcctttt tatccattcc atacgctgaa tggcgaacgc cgtaatacag
     5221 aaacgccgga taataaggag caccttcagg aaaacggcga ttcccctgcc cgtttttctg
     5281 gtcaggggaa attttcacca tcagtccggg gcgacgcgtc gtttttttgg gaacgtaata
     5341 accaatggag cgggccagac gcccggtctg atatcccggg ttctctcccg gaccggagcg
     5401 tccgcgttta atcaccagac gtctggcata acgcatgtaa acgcgcccga tttgcacaaa
     5461 cgcccgacgc agacgggcgc gattaaactc cagctcctct ggttgtttaa agtcgacgtg
     5521 taaaaatgct gtctgattca tggcattcac cccgtcgtcg cgctgtacgc agttcttcac
     5581 attccagtaa taaaaaacgc cgctgaccgt tcaggtcacg tattcgccgg atccggtact
     5641 cttggccgta ataaaccact tcgtgatctg ccgtgatgtc gtgccggaaa cggatcgtaa
     5701 aataatgcgt aacgatattt tctgtctgca ctgagccctg ataagcggca gcgcctggct
     5761 gagccacctt tgcccagaca tcaaacgact ccggatacgt tggctccgta ccaaaatcag
     5821 cggtgggttc atccacacga aggcggatct ttatccggcg attcagctct ccgggatccg
     5881 gtaaaaggta agtggcactg gtctgacttt gcctgatttt catagcggga caatcctgta
     5941 agggccagca agccatctga aactcattgg cgtttccagt ttctccacat cggtgatcgt
     6001 tgagcgattt tcataaaaat gactgaccag catcagcatt gccagacgaa cgtcatcagt
     6061 cagatgcatc ccgtcaggat catcttccgg aatcgtctct tccggtgcat acaacttcct
     6121 gttcaggtat gtctctgttt ttttctgtac cgcctgtgcc agcagtttca gaaaatcgcc
     6181 gtcactgtac aaaccatcat cgagccggag atgagattta atctcctctt ctttcaggag
     6241 catattttcc tcctgtgccc gcgattacgc gggcacaaaa aaccgcatta cgcagcggct
     6301 ttctggcggg ttgcagcccc aattttcatc agcttaatcg cctgagaatc caccagcata
     6361 ccgccggttc gcttggtggt ataaaaaccc acgaacggtt tgttggtgta cgggtcacgc
     6421 aggatacggg taccgatgcg atcaacgatg gtatagccgc gtttgaagtt cccaaacgca
     6481 atggcttttg catcggcggc aatatccggc atctgctcat tctcaacgat gccataccct
     6541 gccagagaag aaggctgacc cagctcaata cccggacgcc acaaataatt tccgtcgtta
     6601 tccttcagca gacgaatggc aaacaggctg ctgttgttca tcatgaactt cgcgccgctg
     6661 cggtgcgcct tacgcaaggt gtaaatcagt ttaatgatcg catcagcggt cacgccggaa
     6721 gccgcgccgg aagcaatgtg ctgaagtttg ccaaacgcac gggtcttgtc atcttcatcg
     6781 gtggactcat aagccagaaa accttttggt tttttgctac cgtcgccact ggtaaaggca
     6841 atttcttcct gttcggcaaa ttccagcgcc agctcactgt tgatccagtc ttccacatta
     6901 aagaaagcat catcgagcat tttctgtgtg gcctgcgggt tgccgtagat ttcccccata
     6961 aagggctcaa tcagtcccag ttttgaggtg gcggtttcct gacgcgcttc cgtttccccc
     7021 acccatccgg acgtcgtgcc gcccagattc accagttttt tataatccga gccacccaga
     7081 gtgattacgg tggcctcctg gcgcatcacc acttcatctt tcagcaacgt cagaatggtg
     7141 cgatccagtt cttccggaat ggcataacca ccatcttcat cattacccac ctgcagtgcc
     7201 tttcgctcaa gctcacgcag gccatcttca cgccccttgc gcataaaacc gataaacgct
     7261 tctttatgtt ccccggcaac tttattttgc gtaccacctg ccggacgctt aacttcagcc
     7321 agctcagcct caagatcgct tttgagattt tccagttcag tcagttttcc gttgagggtt
     7381 tccacttctc cgttcagtgc agagcccaca tcctcagcgt cgcgcaggga cagtgcgcca
     7441 tagcccccgg ccatgaatgc tttggcctgg gtacgggaga gtccgacatc acgcaggact
     7501 ctttcgattt ttttctgttc ggggatttcc ccgcgggcca gtgcgttctt gacgtcgctg
     7561 atccgcgcct cgtcgttaga cgggaacgtc accagactga cttcccagag gtcgatttct
     7621 ttcagcagaa aggcttcttt gctccggtcg tattcccagt ctttcaggac gtacccaata
     7681 gaaaggccgg ttaacgaacc ggccttcatg tgtgcatgtg cacgttttgc gaggggatca
     7741 tcatcaataa gcaaccgtcc cctgacgtaa agcccgacat cgtcttcctt catttcggtg
     7801 taaacaccga tgggttcatc catgcggtgc tgccagagca gcgcaggtaa cgcttttctg
     7861 tcactccacg cccgcaggga agcagcaaat gccccggaca tcaccacatc atcgtggctg
     7921 tcctttacac caaagacgga gccatgccct tcaaactcac cggagtcact gacagatttc
     7981 agactcagcg gtacatcaag acgttgtttc gtctgcattg gcgttatcct tctgcttacc
     8041 ggctttactg ccatcggagg gtttcgtggt catgttcatc ggtgtgagat agacatcccc
     8101 accgggacgc ggattcatat cttccaggtc gcggcagtca ttgggagagt aaatccccca
     8161 gttaatcccg gtggcgtagg cttcaaaacg ggatttcata tccccgcgca gtaacgcccc
     8221 ggcgttaaat ttggcgtaaa acttcccctg tttgctctcc ctgaccagcc ctgtattgat
     8281 ccgttgttca atacgggtca gatacggcac aagggaatag ttgataaatc cgagccccag
     8341 ctcttcaata ttgttgaaag tggcgcgatc ggtgttctgc accatgtgca gcggcacgcg
     8401 gaaaagacga cagatttctt ccaactgaaa cttgcgggtt tccaggaact ggctgtcctc
     8461 ggcattcagt gccaccggct tccagtccag ccccatttcc agaatcatcg gacggtgcgc
     8521 attacccagc ccaagatgac gctcctcaaa atccctcttc atgcgctcat aagcatccgg
     8581 cgtgagcttt tgttccgtgc gcaacacacc ggatgtcacc gcaccattac caaacaacct
     8641 ggcaccgtgc tcctcggttg ccgctgccag tgaaatggcc tcgcgcgcat atgcaatggg
     8701 attcagcccg accagtccgt ccagcgtcag ggtgcgcaca tgccagattt catcctgggt
     8761 caacacatcc acggaaccat ccggaaacgt cacctgataa accggctgcc actggctgtt
     8821 cagcttcggt tccacacagc ccggatctat cggaagaagc tccacaactt cccccagcgc
     8881 cttcaccttg taagcgtaaa aattcccccg cagacacagg cagacgataa ccagctccca
     8941 gaattcctgc ggcgtcatgt agccattggg ttttgctgaa atcagcttat gcagccgttc
     9001 atccaccgcc cgtgttttaa gggtgccgct gattttgtag agactgcagg gcagcatacc
     9061 aacagactca gccagcaccc tgacgcagga atagaccgcc gtcagccgca tggcccgctg
     9121 gctgctgatc cgctttccgg tataggtgtc gtatgacaac cccagctctt ccgcaagcat
     9181 cccgggcgtt gtgacggggg tgttattttt gcgttgaaaa agcccctgga aaaacattac
     9241 tcacctccgg aggcgacccg gtgaccgcga tcgagataac gcgcaaccag ccacgaccag
     9301 cacagacaca gcgccccggc aacaacaaaa cctgccgggg gataaatcag ccatgtgcca
     9361 tacgacagca aaagcgcccc cagcacgccc gccagtggcg tgagaattat cagaaacata
     9421 atgacctcgg ttaaagcgag cgaataccaa cgctgaccag atgctcagac agatccggct
     9481 ccggttcacc gccattgacc agcatccggc tcattgctgt aaacatcgca acagggccgt
     9541 cgattttggc ttccggcgtg gatttgttcg ggaagatatt gtcgtttttg tccggtttca
     9601 ccgtaacgtt agacatcatc cagttcatga ccgggtgatt actgtgatgg aaacgcccgg
     9661 catagaccag tgattccgtt tccttcatgg cctctgacag attgcggacc gtctgcggaa
     9721 cttccaccag cggtatacct tcttcagcca gcgccagact gaactgcatt gcgctccacg
     9781 ggtcaaatcc cagttccctc aggttttcac cgccaatcca ttccagtaag tcacttttta
     9841 tctgagcatg atcgataaca tcaccatccg tcagaatcag cttatccatc tccgcccact
     9901 tccggtaaag ttctgcctgc tgccgcgagc atcgttccag ccgtccttcc ggaagccaga
     9961 atttaaaatc ggcatgaaca tgcccgttat ccgttcgcca gagttttgcc gccgcacaga
    10021 tatcaatctt atgagcaagg tcaacgccga cccacatggg atacgttttc agctcatgtc
    10081 gtggggcaat gtattcgcac ttctcccact taatcatgtc catccaggca gactctgctg
    10141 ttacccacac attcatgtgt ttggtaaaaa aattcacccg cgcagagacc tgttctttcg
    10201 cttttttcgc caggcgacgc agatcatccc agcgtttaca gatgcccagg ccgggattcg
    10261 ctttctgcca gaccgtttca tcaaacggat catctccctc atcgagggtg taaataatcg
    10321 caaagtagga gtcgtctttt accgcgccct ccacatcgct gttatagcca cgcaatacct
    10381 tgatggcgta atcacgctgc tcgtaacaaa tcccttcctt gttaaaccct gccgtggtga
    10441 taccaaataa aagggactgc agacgggcac cggttgccgt ttccagaacg tcccacacgt
    10501 cacgggtttt atgtgcatgc agctcatcaa taatggcgca gtggatgttc agaccatcca
    10561 ggttgtttgc atctgaagaa agcggttcaa attttgatgc gctctgctcc tgataaatcg
    10621 ccagcttgtt gaaatcaaac aaccgcccga gtgtcgaccg ggcttttctg accatatttt
    10681 tggcgtcttc aaacacgatt ctggcctggt cacgcgtggt tgcggctgaa tacacctcag
    10741 ccccgccttc accatctgcc cccgtcatat acagaccgat acctgatgac agggttgatt
    10801 ttgcgttttt acgggcgact tcgttgtacg ctgtccggaa acggcgcacc atcaccgggc
    10861 gtccgctgcc atcgctgcgc atgacaactt ccccggtctc ttcattcacc agcggaatga
    10921 caaaaccaaa aatattaatg aggataaaca catgccagtc catcaactca atgggctgac
    10981 ctgccagcgc cccttttaca tggggcacaa atttgtagaa attcaggatg tgctgcgcac
    11041 ggggttcact gaaataaatc ccccactctt cgccgtactt cagatcatca agaaaacgct
    11101 ggcaggccag acggacaaat tcaccagcga caatttctcc tgcaacaaca cgttcggcgt
    11161 agcggatccc gtcagccact tttgccatca gtctctcgct tttaaaagtt ctgccagcgg
    11221 atcaacatca tccggtccgg cgatatttac tttcacccgg cttgccggtg acataccaaa
    11281 ttctgcaagc atcgcccgga tccgcttcca ggcatccgct ttcatcgcag cagccgggtg
    11341 tgccttaatc agcacatcac cgttctgcgt ttccgtgcgg taggtataac cctcaacatc
    11401 gagggtttcg cagtgatgcc ggtattcggt gtaggcttcc accagcaact cgagtgcacg
    11461 cgcatcaagc tgagaaatga tcccttccgc attcagctct tccgccattc gcctgaacca
    11521 gtactttccc tgcgccccta aatgctgcgg aattttaggg agaccttttt cgtccttttt
    11581 agcggttttt ttggcgtctt taacgggacg ctttgaggga ttgcctcgta tcaaatgcag
    11641 gcgtggcggg gttttcgggg gtcctgacat aatcgatttt acctatcaat cgcttgatcg
    11701 cattcccaaa aaaaagtttt cgaacctgcg gcgatgcgag gaagggttgg cgggcggtcc
    11761 cggacagcca gggctgcagg gatttgaccc gcccctccct acaagtgaga ataattatca
    11821 cctgattcgt tcgcgcgctg ttttcgcttt atggcagggc cagcacagac tctgcaggtt
    11881 gctgtctgcg tctgttccgc catgcgcttt cgggatgatg tggtcgacgg ttttcgcctc
    11941 gctcaccaca ccgacacgca gacacaactg acacagacct ttatcgcgct tcagaatacg
    12001 ggcacgaatc accgtccatt ttgagccata gccacgctgg tggcggctca gtccgcgctg
    12061 gtgctgcacc cagccttcac cgcgatgctt atcgcagtaa cccgaactgt ctgtggttgt
    12121 acctgcacat ccacgcttac ggcaggcacg tgggattagt gatggcataa atacctcata
    12181 ccctgcgaaa tgtttaccac gataaaaagg ctacttaatg cactgagtgc ggatatactc
    12241 ctgtgcccct tccagttgca tctgcatcgt catcagccgc tctctgaggg tgaaataatc
    12301 ccgttcagcg gtgtctgcca gtcgggggct ggttgcatta tccacgccgg aggcggtggt
    12361 ggcttcacgc actgactgac agactgcttt gatgtgcaac cgacgacgac cagcggcaac
    12421 atcagcgcgc agagtttcat tttcagcttt cgcatcagct aactccttcg tgtatttagc
    12481 atcgagtgca ttaacatcac gctgtctctg ctgcatgtca gtaatggttg cgttcgccag
    12541 cttcagttct cttgcgtttt tgtcgcgctg ctctttgtag gtaatggcgt tatcacggta
    12601 atgattaaca gcccatgaca ggcagacgat gatgcagata accagagcgg agataatcgc
    12661 ggtgactctg ctcattgctg cccccacaaa cagacttcac gctcaatctc acgacgggtc
    12721 atcagccctt tccattgctt accgccagca tatgtccagc gacgtagctg gtcac
//
LOCUS       DAJTYG010000101        12212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTYG010000101 DAJTYG010000000
VERSION     DAJTYG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.101"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            8..1822
                     /gene="bglF"
                     /locus_tag="OO568_003198"
     CDS             8..1822
                     /gene="bglF"
                     /locus_tag="OO568_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6642446.1"
                     /translation="MHCATRLRFKLKDESKAQAEVLKKTPGIIMVVESGGQFQVVIGN
                     HVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVYVISGIFTPLIGLMAATGILKG
                     MLALALTFQWTTEQSGTYLILFSASDALFGFFPIILGYTAGKRFGGNPFTAMVIGGAL
                     VHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVIPIIFSAWLCSILERRLNAWLP
                     SAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAAGYLWLYQAVPAFAGAVMGGFW
                     QIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIMAQVGAALGVFLCERDAQKKVV
                     AGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGALGATIIGYAQTKVYSFGLPSIF
                     TFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLHFITSKRQPAQGAPQEKTPEVI
                     TPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIAILPSVGEVRSPVAGRIASLFA
                     TLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVGDKVNTGDRLISFDIPAIREA
                     GFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSIIR"
     gene            1838..3235
                     /gene="bglB"
                     /locus_tag="OO568_003199"
     CDS             1838..3235
                     /gene="bglB"
                     /locus_tag="OO568_003199"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR6642447.1"
                     /translation="MTKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGK
                     MEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA
                     GLAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTR
                     YQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLI
                     PEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHN
                     ITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSER
                     GWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQ
                     VNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFG
                     WYAEVIKTRGLSLKK"
     gene            3321..4937
                     /gene="bglH"
                     /locus_tag="OO568_003200"
     CDS             3321..4937
                     /gene="bglH"
                     /locus_tag="OO568_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR6642448.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            4964..6138
                     /locus_tag="OO568_003201"
                     /pseudo
     CDS             4964..6138
                     /locus_tag="OO568_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            6140..6862
                     /locus_tag="OO568_003202"
     CDS             6140..6862
                     /locus_tag="OO568_003202"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6642449.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(6924..7511)
                     /locus_tag="OO568_003203"
     CDS             complement(6924..7511)
                     /locus_tag="OO568_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR6642450.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGMKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(7560..8033)
                     /locus_tag="OO568_003204"
     CDS             complement(7560..8033)
                     /locus_tag="OO568_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR6642451.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            complement(8100..8765)
                     /gene="yieH"
                     /locus_tag="OO568_003205"
     CDS             complement(8100..8765)
                     /gene="yieH"
                     /locus_tag="OO568_003205"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR6642452.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            8929..10266
                     /gene="adeP"
                     /locus_tag="OO568_003206"
     CDS             8929..10266
                     /gene="adeP"
                     /locus_tag="OO568_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6642453.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            complement(10320..10886)
                     /gene="yieF"
                     /locus_tag="OO568_003207"
                     /gene_synonym="chrR"
     CDS             complement(10320..10886)
                     /gene="yieF"
                     /locus_tag="OO568_003207"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR6642454.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(10908..11657)
                     /locus_tag="OO568_003208"
     CDS             complement(10908..11657)
                     /locus_tag="OO568_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6642455.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            complement(11814..12125)
                     /locus_tag="OO568_003209"
                     /pseudo
     CDS             complement(11814..12125)
                     /locus_tag="OO568_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
BASE COUNT         3052 a         2861 c         3098 g         3201 t
ORIGIN      
        1 gagtctgatg cattgcgcaa cgcgattacg ttttaagtta aaggatgaaa gcaaagcgca
       61 agctgaggta ctgaaaaaga cccccggtat tattatggtg gtggaaagtg gtggtcagtt
      121 tcaggtggtc ataggtaacc atgtggccga tgtcttcctg gcggttaaca gcgtggcagg
      181 ccttggcgaa aaagcgcaac aggcaccgga aaatgatgag aaaggtaatc tgctaaaccg
      241 ctttgtttat gttatttcag gtatttttac gcctctgatc ggtctgatgg cggcaaccgg
      301 gatcctgaaa ggtatgctgg ctctggcgct cacttttcag tggacgaccg aacaaagtgg
      361 tacttattta attttattca gcgccagtga tgccttgttt gggttcttcc cgataatcct
      421 gggatacacc gcgggcaaac gcttcggcgg taatccattt actgccatgg tgattggtgg
      481 agcgttagtg catccattaa ttctgactgc cttcgagaac gggcaaaaag cggatgcgcc
      541 ggggctggat ttcctgggta ttccggtcac attgttgaat tactcgtcat cggttattcc
      601 cattattttt tctgcctggt tgtgcagcat tctggaacgc cgacttaatg cgtggttacc
      661 gtcggcaatc aaaaatttct tcacaccatt gctatgtctg atggttatca cacccgtcac
      721 ctttctgctg gtggggccgc tatcaacctg gataagcgaa atgattgccg ccggttatct
      781 ctggctttat caggcggttc ctgcatttgc gggcgcggta atgggcggct tctggcaaat
      841 cttcgtcatg ttcggactgc actggggcct ggtgccgctg tgtatcaata acttcaccgt
      901 gctgggctac gacaccatga tcccgctgtt aatgcccgcc attatggcgc aggtcggggc
      961 ggcgctcggc gtcttcctct gcgaacgcga tgcgcagaaa aaagtggtgg cgggatcagc
     1021 ggcgttgacg ggtctgtttg gtatcaccga accagcggta tatggcgtca acctgccgcg
     1081 taagtacccc tttgttatcg cctgtatcag tggagcattg ggggccacca tcattggcta
     1141 cgcgcaaacg aaagtttact cctttggttt gccaagtatt ttcaccttca tgcagaccat
     1201 cccgtcaacg gggattgatt tcaccgtctg ggccagcgtt attggcggtg tcattgccat
     1261 aggttgcgca tttgttggta cgttgatgct tcatttcatc accagtaaac gtcagccagc
     1321 gcagggtgcc ccgcaagaga aaacaccaga ggttattaca ccacctgagc agggcggtat
     1381 ctgttcaccg atgacgggag agattgtgcc gctcattcac gtcgctgata ccacgtttgc
     1441 cagtggcctg ctgggtaaag gtatcgccat tctgccctcg gttggtgaag tgcgttctcc
     1501 ggttgcgggt cgaattgctt cgttgttcgc cacattacac gccattggca ttgagtcaga
     1561 tgatggtgtg gagatcctga ttcatgtcgg tatcgacacc gtaaaactgg acggcaaatt
     1621 cttttccgct cacgtcaacg tgggtgacaa ggtcaataca ggcgatcggc tgatttcttt
     1681 tgatatccct gctattcgcg aggccggatt tgatctgacg acgccggtat taatcagtaa
     1741 tagcgatgat tttacagacg tattacccca cggcacggcg cagataagcg caggtgaacc
     1801 gctgttatcc atcattcgct aacgataaaa ggagttaatt acgaaagcat ttccagaaac
     1861 atttctttgg ggtggcgcaa cagctgccaa tcaggtggaa ggtgcctggc tggaagatgg
     1921 caaagggatc acgacctcag atttacagcc tcatggcgta atgggaaaaa tggaaccgcg
     1981 catcctgggg aaagagaata tcaaagatgt cgccatcgat ttttatcacc gttacccgga
     2041 agatatcgcg ttatttgccg agatgggctt cacctgtctg cgtatttcca ttgcctgggc
     2101 gcgaattttc cctcagggcg acgaagccga accgaatgaa gcggggttag cgttttacga
     2161 tcggctgttt gatgaaatgg cgcaggcggg gatcaagccg ctggtaacgt tatcccatta
     2221 cgaaatgcta tatgggctgg tgaaaaacta cggcggttgg gctaatcggg cggtcatcga
     2281 tcacttcgag cattacgccc gcacggtctt tacccgctac caacataaag tagcgttatg
     2341 gctgacgttt aatgaaatca atatgtcgtt acacgcgcca ttcacgggcg tggggctggc
     2401 agaagagagt ggcgaggcgg aagtttatca ggcgatccat catcaactgg ttgccagtgc
     2461 gcgggcagtt aaagcctatc atagcctgat ccccgaagcg aaaatcggca atatgcttct
     2521 cggtgggctg gtttaccccc tcacctgcca gccacaggat atgttgcagg ccatggaaga
     2581 gaaccggcgc tggatgttct ttggtgatgt tcaggcgcgt ggccagtatc ccggctatat
     2641 gcagcgtttc ttccgcgacc acaatatcac cattgagatg actgaaagtg acgcagaaga
     2701 tttaaaacat actgtagatt tcatctcttt tagttattac atgactggtt gtgtttccca
     2761 cgacgaaagc attaataaaa atgcgcaggg caacatactg aatatgatcc ccaatccgca
     2821 tctgaaaagt tcagagaggg ggtggcaaat tgatccggtt ggattacggg ttctgttaaa
     2881 tacgctttgg gatcgttatc aaaaaccgtt atttattgtc gagaatggat taggcgtaaa
     2941 agacagcgtt gaagcggatg gttcgataca ggatgattat cgaattgcct atttaaacga
     3001 tcacctggta caggtaaatg aagcgattgc cgatggtgtg gatattatgg ggtacaccag
     3061 ttgggggcca attgatttag tcagtgcatc tcattcacaa atgtctaagc gctacggctt
     3121 tatttatgtg gatcgtgatg ataatggcga aggaagcctc acaagaacgc gtaagaaaag
     3181 cttcggatgg tatgcagaag tgatcaaaac gcgggggctg tcattaaaaa aataaccaat
     3241 aaagcacctt aattatcgtc gcattcagaa cagtctggat gcgatacgtt aattctttct
     3301 ttgcaccata aagggatatt atgtttagac gaaatcttat tacctctgcc atcttattaa
     3361 tggcaccgtt agccttttct gcacaatcat tggctgaatc attaacggtg gaacaacgcc
     3421 ttgagttatt agaaaaagcg ttaagagaaa cgcaaagcga actcaaaaag tataaagatg
     3481 aagagaagaa aaaatatacg ccagcgacgg tgaatcgtag cgtaagtacg aatgatcaag
     3541 ggtatgccgc caatccgttc ccgaccagta gtgccgcaaa acctgatgct gtactggtca
     3601 aaaatgaaga gaaaaatgcc agtgagacag gctcgattta ttcttccatg actctgaaag
     3661 atttcagtaa gtttgtgaaa gatgaaattg gctttagtta caacggctat taccgttctg
     3721 gttgggggac cgcctctcat ggttcaccta aatcatgggc gattggttct ctgggccgct
     3781 ttggtaacga atactccggc tggtttgatt tgcagttaaa acaacgtgtc tacaacgaaa
     3841 acggcaaacg ggttgatgcc gttgtgatga tggatggtaa cgttggtcag cagtactcta
     3901 ccggctggtt tggcgataac gccggtggcg agaactttat gcagttctcc gatatgtacg
     3961 ttaccaccaa aggtttcctg ccctttgcgc cagaggctga tttctgggtg ggtaaacacg
     4021 gtgcgccgaa aattgaaatc cagatgctgg actggaaaac gcagcgtact gatgccgcag
     4081 cgggtgtagg tctggaaaac tggaaagtcg gtccgggtaa aattgatatc gcgctggttc
     4141 gcgaagatgt cgatgattac gatcgcagcc tgcaaaacaa acagcagatt aatacccata
     4201 ctattgattt acgctataaa gatatcccgt tatgggataa agcgacctta atggtgagtg
     4261 gtcgttatgt cacggcaaac gaaagcgcat cggaaaaaga taatcaggat aataacgggt
     4321 attatgactg gaaagatacc tggatgttcg gcacatcttt aacgcagaaa tttgataaag
     4381 gtggcttcaa cgaattctcc ttcctggttg cgaataactc tatcgccagg aactttggcc
     4441 gttatgctgg cgcaagtcca tttaccacct ttaatggtcg ttattatggt gatcacaccg
     4501 gcggaacagc agttcgtctg acctcgcagg gcgaagccta tatcggcgat cattttattg
     4561 tggctaacgc gattgtttac tccttcggta acgatattta tagctacgaa acaggcgcac
     4621 actctgattt cgaatctatt cgtgcggttg ttcgcccggc ctatatttgg gaccaatata
     4681 accagacagg tgttgaactg ggctatttca cccagcaaaa caaagatgcg aatagtaata
     4741 aatttaatga gtctggttat aaaaccacgc tcttccatac ctttaaagtc aataccagta
     4801 tgttgacctc gcgtctggaa attcgtttct acgccacgta tatcaaagcc ctggaaaacg
     4861 aactggatgg cttcaccttc gaagacaata aagacgacca gtttgctgtc ggtgcccagg
     4921 ctgaaatctg gtggtaatgg aacttaatga aaggaatatt acgatgaata taaaaattgc
     4981 tgctttaacg ctggctattg ccagcggtat ttctgctcag tgggccatag cagcggatat
     5041 gccagccagc ccggcaccca ctatcccggt gaaacagtat gtgactcagg tcaatgccga
     5101 taacagcgtg acctttcgct actttgcccc tggggcaaaa aatgtctctg tagtggtggg
     5161 tgttccggtt ccggacaata ttcacccgat gaccaaagac gaagcaggag tctggtcgtg
     5221 gcgcacacct atcctgaaag gcaacctgta cgagtatttt ttcaatgttg atggtgtacg
     5281 cagcattgat acaggcaccg caatgactaa gcctcagcgc caggttaact ccagtatgat
     5341 tctggtgcta ggcagttatc tggatacgcg ttctgttgcg catggtgatt tgatcgccat
     5401 aacttaccac tccaacgcat tgcaatctga acgtcagatg tatgtctgga ccccgccagg
     5461 atacaccggc atgggcgagc ctttgccagt gctctatttc tatcacggtt ttggtgatac
     5521 tggacgttcc gctatcgatc aggggcgtat cccgcaaatc atggatacct gctggctgaa
     5581 gggaaaatta aaccgatgct ggtggtgatc ccggataccg aaaccgatgc gaagggcatt
     5641 attcccgaag atttcgtgcc tcaggaaaga cgtaaagtct tttatccgct gaatgctaaa
     5701 gcggcagatc gcgaactgat gaacgatatt atcccgctga ttagcaagcg ttttagtgtc
     5761 cgtaaagatg ccgatggtcg cgcgctggca gggctttcac aaggcgggta ccaggcgctg
     5821 gtttccggaa tgaatcatct ggaaagcttt ggctggctgg ccacattcag tggtgttacc
     5881 acgacaaccg taccggatga aggtgtcgcg gcccggctga acgatccggc agctattaac
     5941 cagcaactac gtaattttac tgtggtcgtt ggtgataaag atgtcgtaac cggcaaggat
     6001 atcgccgggc tgaaaactga gcttgagcag aaaaaaatta agtttgatta ccaggaatat
     6061 cccggcctga accatgaaat ggatgtctgg cggcctgcct atgcagcctt cgtacagaaa
     6121 tttaaaataa ggcattaaga tgaaattaat cattaccgaa gattaccagg aaatgagccg
     6181 tgtcgcggca caccatctgc ttggttatat gtcgaagacg cgtcgtgtta acctggcaat
     6241 taccgccggt agcacgccca aaggcatgta tgaatacctc accaccctgg ttaaaggtaa
     6301 gccttggtac gataactgct atttctataa tttcgatgaa attccctttc gcggcaaaga
     6361 gggagaaggc gtaacgatta ccaatctgcg taatctgttt ttcacccctg cggggatcaa
     6421 agaagagaat atccagaagc tcactattga taactaccgc gagcatgatc agaaactggc
     6481 gcgtgaaggc ggcctggatt tggtggtgct gggattaggt gcagatggtc atttttgtgg
     6541 caatttgccg aatacgaccc atttccatga gcaaaccgtg gagttcccga tccagggaga
     6601 gatggttgat attgtggcgc atggtgagtt gggcggcgac ttttcgttag tgcctgacag
     6661 ctacgtgacg atggggccga aaagcatcat ggcggcgaaa aacttgctga taattgtcag
     6721 tggcgcaggt aaagcgcagg cactgaaaaa tgtgctgcaa ggtccggtta ccgaagatgt
     6781 tcctgcttcc gtgttgcagc tgcatccttc gctgatggta atcgctgata aagccgctgc
     6841 cgcagaatta gcgttcggtt aagctgaaga aataaggccg gatgtatgaa ttacgctccg
     6901 gcctgttctc attatttaaa taactacgaa aaatcacccc acagtctcag cttgccgcag
     6961 tggagacagc ggaagagata accctgacaa tcaccaccct tttgcaggca tttagctaga
     7021 tcagaatttc taataccgaa attctcacaa tcttcttcaa ggttggcaaa ttcatcgagg
     7081 cgatctttta tatcattcca gcccacatag ccgataaaag cacagaaatc gccacaatgc
     7141 gcaagccaga attcctgttg ccatccatga taacctggcg tgcgttcaac caactctttc
     7201 agcatttcat caggatatgt gttcttcata ccggcaaatt cgtcctcttc atcatactca
     7261 aattcaacac cttctatgct ggcatcatcc tgaaaactac ctgcaaactt ttcagcagca
     7321 gaaccgtccg caatacacaa cggacagaga tgttcaactt catcaacgca ataaaaggga
     7381 cccgagtaat aaactgacgt ctgttgttca cagcaatcgc actctacggt tttatcctgt
     7441 tcaaatgcgc ctgtttccag tggcttggga tgatatttga attggggtaa cggcctgata
     7501 ttttgagtca tagtaacatc ctgtctgctt ttgagtagcc caaagataaa gtatcccgct
     7561 taacttaaag agtctgtttc aggacatgaa atatccaatc cgggcagacg ggtaatgatt
     7621 ctgctcatca ccacacctgc cagcaatgca ggaatgatgc ggacaacgag actgtcacca
     7681 gaaagaatgt tttcaaacaa ctccgaggaa gccatgccct taatatgtac aataactgca
     7741 caatagatct cggtgataac gacgccacct atgccaccag ccagacaacg ataatttttc
     7801 tgtgacccga tcttttccgg cagacaggcg accatcacgc cggttaacaa tgcaggaata
     7861 gctcccgtag taatcgacaa taagagaaac aatggaagta cctgtattat taccaaaaga
     7921 accagcggtt cttttgcgaa gaatatgtag aggacaagaa acagaacacc aattaacggt
     7981 cctaaaaccg caaaataaag tccgtgatgt attacccgac gtgaaacaga catatcaaac
     8041 tccctttact tacctttgag cgccaattta agcggggtaa gtgaagggag tgtgaagaac
     8101 tatgccgtaa tatcccaacc acgcgctttc cacagttccg gtaactgcga aagatgggta
     8161 aaggtggtga ctttcgggtg aacgatcggc ttattgtgcg ggtcggcgca gaagtagaac
     8221 acttccatac ctgcgtcgat accagattgc gcaccggcaa ctgagtcatc aaccagaatg
     8281 cagttttcta catttacatt catcgctttt gccgcatgga acattaacgc cgggtctggc
     8341 ttccagcgct gaatatcgta gccgctgaac agtttatccg ggaagtagtg caacatattc
     8401 agcttgccca tagaatgctg cattttgtta tttgggccgt tagataccac acacattggc
     8461 gcagtgatcg ctgacaggag cgctccagcc ccttcgatgg cttccagttc tgaatcgaac
     8521 agccgagcga cttctgcacg gtaaacgtgt tcagcttctg ttttcgctaa cgtaacacca
     8581 tgttcaaggg aaacaatatc gataatttcg tacagtttta cacctttgaa acgtttgaat
     8641 acctcttcag gatcgagcgt aataccaaat tcctgaaaca tcgttacata tgcgcgggag
     8701 caaatgactt cactgtcgac cagcgtaccg tcgcagtcga aaaataccgc ttctatccgg
     8761 gacatgcctt tcctttttaa tcagtttaac gttttcgtag agccgatttg cgcaacgcaa
     8821 tcgttgccgt ataagtaaat ccaataaaaa gtctctcaca aacgcgccta ttgtcgcatt
     8881 ttggtatacg atagcgacgg attttccctc cttgttcgga aatagataat gagtcatcaa
     8941 cataccaccc agacttctgg tcagaggatg ctggaacgcg tttttaaact gcgcgaacat
     9001 ggtactacgg cacgaactga agtgattgcc gggttcacca ccttcctgac gatggtctac
     9061 atcgtatttg ttaacccaca aattctgggc gttgctggca tggacaccag tgccgtcttc
     9121 gtaacaacct gtctgattgc tgctttcggt agcattatga tgggattgtt tgcaaacctt
     9181 ccggtggcgc tggctcctgc gatggggctt aacgcctttt tcgcttttgt ggttgtccag
     9241 gcaatggggc tgccgtggca gatcggtatg ggcgcaatct tctggggcgc tgtcggactg
     9301 cttcttttga ccattttccg cgttcgttac tggatgattg ctaacattcc ggtaagtctg
     9361 cgtgtgggta ttaccagcgg tatcggcctg tttatcggca tgatggggct gaaaaacgca
     9421 ggtgtgattg tcgctaactc ggaaacgctg gtgagcatcg gtaatctgac ttctcacagc
     9481 gtacttctgg gtatcctcgg ctttttcatc attgctattc tggcctcgcg caacattcac
     9541 gcagcggtgc tggtttctat cgtggtgacg acgctgctgg gctggatgct gggtgatgtg
     9601 cactacaatg gcatcgtttc tgcgccgccg agcgtaatga cagttgtggg tcatgtagat
     9661 ttagccgggt cgtttaacct cgggctggca ggggtgattt tctctttcat gttggtcaac
     9721 ttgtttgact cctccggtac gctgattggc gtgaccgata aagcaggtct ggcggatgag
     9781 aaggggaaat tcccgcgcat gaagcaggcg ctgtatgtcg acagtatctc ttccgtgacc
     9841 ggttcgttta tcggtacttc ttccgttacg gcttatattg agtcctcttc cggcgtatcg
     9901 gttggcggtc gtaccggtct gacggcagtg gttgttggtc tgctgttcct gctggttatc
     9961 tttctgtcgc cgctggcggg aatggtgcca ggctacgctg cagctggcgc gttgatctac
    10021 gttggcgtgt tgatgacctc aagtcttgct cgcgtgaact ggcaggatct tactgaatct
    10081 gttccggcgt ttattaccgc cgtgatgatg ccgttcagct tttcgattac cgaaggtatt
    10141 gcgctgggct ttatctccta ctgcgtgatg aagattggta ccgggcgtct gcgtgacctt
    10201 agcccgtgcg taatcatcgt tgcgctgctg tttatcctga agattgtatt tatcgacgct
    10261 cattaaaatc gatagcgcat taaaaacccg ccatgcatga ttactggcgg gttttttatt
    10321 tagatcttaa ctcgctgaat aaactcacca aatgcggtca attgcccggt caggtggtcc
    10381 agcgtaccct gatcaatcac ttctccggtt tgcggatcaa ctttgttctg aatcacgccg
    10441 cccataaatt ccggcttgtt catcaccatt gcatcgagga aaaccagaat ctggcgcagg
    10501 tgatactgac agcgcgcgcc gccaatcacg cccattgagc tggtctgaat caacaccggt
    10561 ttaccggcca gcggttgatc cggcaggcgg gaaagccagt cgatggcatt tttcagccca
    10621 cccggtaccg agtagttata ttccggcgtg acgatcacca caccgtcagc ctgacggatc
    10681 tgttccgcca gagcttcaac cgttgctgga aaaccttctt cctgctgtac gtcagcgtca
    10741 tacaggggaa tgtcggcaat ggaaggtaac gcattgactt ccatgctcgc cggagcaatt
    10801 ttcggcaggg tacgtgcaac catgccatta aatgagcctt tgcgcaggct ccccagtaac
    10861 gtaaccacct gcaatttttc agacataatg actcctgtgg ttgtctatca atttggcgaa
    10921 aaagacttca cggtagagag ctgggcaaaa cgcatcatcc ggctggtagg attgttggcg
    10981 cggctgtgaa tatccggcag gcttttccag ctgtgtttgc catctaactc ccagacactg
    11041 agcttttcaa tcgcgggcgt gaccgccacg gaccacacca gcacgtcctc cgcgtcgcat
    11101 aacgcttcta catgatttac atgagcacat ttcaggcgtc cggcaatggg cagcagctgc
    11161 agatcgcgcg gatcgtcatt caaaacatca ccggttagtt ttagcacgct tcggcgcagc
    11221 gtaatgagct gcgcccgcgc ttcgttagga tcgttttgtt tactgatcca tagcgattca
    11281 ttgctggaaa aggtgtggtt atcgggcgcg tgtgggctat gaaacccgcg cgtcgcacgc
    11341 tgtagttcca tatcgaggcc acattcacct tcggttgtta acgccacgcc aaccatattc
    11401 ccggcatagg aaatagaaaa cgaaggcaaa tttttatcac tgaaaaccgg tttacctttc
    11461 ggcagggtga cgatttccgg caattcgcca atgccataca gcatgaacat cagttctgcg
    11521 agtaaacctc tggacgccag aaaacggctt tgacgatgag ggggaatgtt tcgggcttct
    11581 tgatgacact gggaggggag acgaacagaa atcagatgtc cttccgttaa aatccctcgg
    11641 gcaaagtgag tcgccatttt tcgctccatg cttaatggtc cattatgaat acaagagtaa
    11701 tcatttactg gaatgatgga cagactaaag cggctaaaag ttcgattcag acaagcttat
    11761 tacaactttt ttacatttta ttgtgaaatt gggtcgcgtt tcatacgatt cggttatgcc
    11821 atcgacgcgt aaaggttttt aatggtttcc cgtaaccaga cgatcttcgg attatggctg
    11881 ttccgtttat gccacagcag ggtaaaagga acttccagct ttttttgctg gctttcgtca
    11941 aacgggagag gaagagcaac caacggcagt tgatggagtt gattgtagta ctgacagtag
    12001 cgcggcgcgg tcgccagtag cagattgtcg ggttgcgctg ccataaacag tgactgctcg
    12061 aattccggca ggctcatagc aatcgtgcgt tcgcgcccca gctcctgtaa cacattgtcc
    12121 agcgccctga ggtagcctga gtttaacgga cactccttcc tgaaataggt aagcgcccca
    12181 tcagcgacgt cttgtgaaaa ttgtcctgtc tg
//
LOCUS       DAJTYG010000102        12177 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTYG010000102 DAJTYG010000000
VERSION     DAJTYG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.102"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..441)
                     /locus_tag="OO568_003210"
     CDS             complement(<1..441)
                     /locus_tag="OO568_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6642456.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFA"
     gene            complement(472..1164)
                     /gene="fimC"
                     /locus_tag="OO568_003211"
     CDS             complement(472..1164)
                     /gene="fimC"
                     /locus_tag="OO568_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6642457.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1365..1907)
                     /gene="fimA"
                     /locus_tag="OO568_003212"
     CDS             complement(1365..1907)
                     /gene="fimA"
                     /locus_tag="OO568_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6642458.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2388..3254
                     /gene="folD"
                     /locus_tag="OO568_003213"
     CDS             2388..3254
                     /gene="folD"
                     /locus_tag="OO568_003213"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6642459.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3256..3468
                     /gene="ybcJ"
                     /locus_tag="OO568_003214"
     CDS             3256..3468
                     /gene="ybcJ"
                     /locus_tag="OO568_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6642460.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3576..4097
                     /locus_tag="OO568_003215"
     CDS             3576..4097
                     /locus_tag="OO568_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6642461.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4133..5518)
                     /gene="cysS"
                     /locus_tag="OO568_003216"
     CDS             complement(4133..5518)
                     /gene="cysS"
                     /locus_tag="OO568_003216"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6642462.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5692..6186
                     /gene="ppiB"
                     /locus_tag="OO568_003217"
     CDS             5692..6186
                     /gene="ppiB"
                     /locus_tag="OO568_003217"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6642463.1"
                     /translation="MVTFHTNHGDIVIKTFDNKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6189..6911
                     /gene="lpxH"
                     /locus_tag="OO568_003218"
     CDS             6189..6911
                     /gene="lpxH"
                     /locus_tag="OO568_003218"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6642464.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7029..7538
                     /gene="purE"
                     /locus_tag="OO568_003219"
     CDS             7029..7538
                     /gene="purE"
                     /locus_tag="OO568_003219"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6642465.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7535..8602
                     /gene="purK"
                     /locus_tag="OO568_003220"
     CDS             7535..8602
                     /gene="purK"
                     /locus_tag="OO568_003220"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6642466.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8739..9632)
                     /locus_tag="OO568_003221"
     CDS             complement(8739..9632)
                     /locus_tag="OO568_003221"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6642467.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9629..10276)
                     /locus_tag="OO568_003222"
                     /pseudo
     CDS             complement(9629..10276)
                     /locus_tag="OO568_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10279..11511
                     /gene="allC"
                     /locus_tag="OO568_003223"
     CDS             10279..11511
                     /gene="allC"
                     /locus_tag="OO568_003223"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR6642468.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11522..>12177
                     /gene="allE"
                     /locus_tag="OO568_003224"
     CDS             11522..>12177
                     /gene="allE"
                     /locus_tag="OO568_003224"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR6642469.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPAEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     "
BASE COUNT         2953 a         3157 c         3104 g         2963 t
ORIGIN      
        1 tgcaaaatca aagttaattg tggcatcagg tacagctttc agtaaattga tgcatttatt
       61 gttttgctgc tgggctaact cgggaaatgc actgctatta aggccaattc gttcaaggag
      121 tacctggtta aaacagggca ttaacccacc tgatttatca cctgtatttt ctgttgtcga
      181 ttcaaatacg atatcctgtg aaccaatgaa ctcatcatta cgccagagat ccacccgata
      241 aacgcccgca ggttgatgat tacctctctc aaagcgcgat aaatcagcaa cggatgtgcc
      301 attctctaat aaaaatgccg ggttgaaata actttccgca tagcttaaaa aggagggtaa
      361 aatggcaaga gatgaataac aaattccggc cagacaccag gtatacctct gcggaatatc
      421 cgtagtagtg ggtattttca taattagctc tgatattgtc agatgattcg gttagtttaa
      481 gttcacttct ctgaccgggg ttactgaacc ataatcatta acggtctgaa atttcagctt
      541 tccctgcact cctgagggta agggaatttg tgaactaact ctgggtgccg ccatagcatt
      601 ccccaacttt tggctgccca cttgcaggtt aacgagggtg acataaaaag gtgatggatt
      661 atgaacattg atatagttac cggatcgcga aaacttgagt gtgtccggcg cttctgcggg
      721 taattcttgt aattgaattg gacggagaaa taatttcatg cgcgataaaa tcgccagttg
      781 caaaacattc ctgccgttca atgcattttt atctaccgaa gggatcgtct taacattcat
      841 ccagaacaga gactcacgat ctgccgccag cggtggaccg gtgtaaataa tacgcaaagt
      901 attttcgctt ttgggttcac taacaaatag tggcggtgta atgatgaatg acttttcttt
      961 tacaccgctg ctgttttcaa tccacgaatt gaccagaaag agctcattgg tatggctatt
     1021 tttaatccat accgcagtct gtttagcatc agcgggataa ataatacgcg tggcacctaa
     1081 tgcgatccct ccggcagcat gggcgctggc cgaaatgatt aaataaaata tcatgagcat
     1141 caataacttt attttagtca tcatagttaa tacgccttgt gtattcctga cggcaaaaga
     1201 ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg catgatacaa
     1261 agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg gtacattact
     1321 ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc gtatctcata
     1381 ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt gcctttataa
     1441 cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt tgagaagcta
     1501 ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc gacacccgtt
     1561 gctgttgttg tatttgtact ggatgcaatg gccaataaat tatcattgat tgcatcagcc
     1621 tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc gttcaactga
     1681 atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc ggtacgatat
     1741 tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc agcattcacc
     1801 agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact acccgtagcg
     1861 gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata tattccttca
     1921 atctcattta tcgactccac atccgtatat aaccgattac tttatttaag acactgatag
     1981 tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca ctctattcta
     2041 aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcaa cacaaatata
     2101 gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg tatttcttaa
     2161 attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg ccatcatgta
     2221 cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac cttcgcaaga
     2281 ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct tctgacaaaa
     2341 tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg gcagcaaaga
     2401 ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa aaagttcagg
     2461 ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg ggtagtaacc
     2521 ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc gggttcgtct
     2581 cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag cttatcgatg
     2641 cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta ccggcgggta
     2701 ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac ggtttccatc
     2761 cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc accccgcgcg
     2821 gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac gccgtggtga
     2881 ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc gcaggttgca
     2941 ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa aatgccgatc
     3001 tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc aaagaaggcg
     3061 caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg ggcgacgtcg
     3121 tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc ggcgttggcc
     3181 cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa tatcatgatc
     3241 cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt tgagctgtgc
     3301 gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat cgcgattgcc
     3361 gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa aatcgtcgcc
     3421 ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc cctttcccgg
     3481 cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc gttcatccat
     3541 gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt acgcacgccg
     3601 ccgttcccct ttgcattggt ttagggttgg gttcgaaagt catccctccg cgtctgttat
     3661 ttgccggaat catcctagcg atgctgcccg atgccgacgt attgtcgttt aaatttggcg
     3721 ttgcttacgg caatgttttt ggtcatcgcg ggtttaccca ttcgctggtg tttgcgtttg
     3781 ttgtcccgct cttatgtgtg ctgattggac gtcgatggtt cagggctggg ctgattcgct
     3841 gctggctgtt tttaaccgtc tcgttgctat cgcacagctt actggattcg gtaaccactg
     3901 gcggtaaggg cgttggctgg ctgtggccgt ggtcagatga acgctttttc gctccctggc
     3961 aggtgattaa agtcgcgccg tttgcattgt ctcgttacac cacagcgtac gggcatcagg
     4021 tgattatttc cgaattgatg tgggtgtggt tgccggggat gctactgatg ggaatgttgt
     4081 ggtggcgcag acgataaccg gatgcgaaaa ctcgcatccg gcaattgcgc aattacttac
     4141 gacgccaggt ggtcccttgc gggccatctt ccagcacgat ccccatctcg ttaagacggt
     4201 cacgtgccgc atctgccgcc gcccagtctt tcgctttacg ggcatccaga cgctgttgaa
     4261 ttaacgcttc aatctcagcc acttcgctgt cgtctgcctg cgcgccgctt tgcaggaacg
     4321 cttccggttc ttgctccagc aggcccaata cagcggaaag tttacgcagg tgagatgcca
     4381 ttgcattcgc cgctgccata tcttctgctt tcagacggtt cacttcacgc gccatatcaa
     4441 acagcacgga ataggcttcc ggggtgttga aatcgtcgtc catcgcttca ataaagcgcg
     4501 cttcaaacgc ttcgccaccg gcaggcgcaa cggttttatc tgtgccgcgc agcgcagtgt
     4561 agaggcgctc cagcgccgca cgcgcctgct tcaggttctc ttcgctgtag ttcagctggc
     4621 tgcgatagtg gcccgacatc aggaagtaac gcacggtttc cgcgtcgtag tatttcagca
     4681 catcgcgcac ggtaaagaag ttacccagcg atttggacat cttctcgcgg tcaaccatca
     4741 ccattccgga gtgcatccag tagttcacat actgaccatc atgggcacag gtggactgcg
     4801 cgatttcgtt ctcgtggtgc gggaacatca ggtctgaacc gccgccgtgg atatcaaagt
     4861 ggttacccag ctgcttgcag ttcatggccg aacattcaat gtgccagcca ggacgacccg
     4921 cgccccacgg agacggccag ctcggttcgc cctctttcga catcttccac agaacgaagt
     4981 ccatcgggtt gcgtttgtca tcaaccacgt caacgcgcgc accagcctgc agctggtcga
     5041 gatcctgacg cgacagcacg ccataagttg gatcggtcgg gacgtcgaac atcacgtcgc
     5101 cgttgtccgc cacataagcg tgacctttgg cgatcagttg ttcagtgagt tcaataattt
     5161 ctgcgatatg gtgcgtcgcg cgcggctcca tatccgggcg cagaatgttc aaagcatcaa
     5221 aatctttgtg catttcggcg atcatgcgat ccaccagcgc cacaaagctt tcgccatttt
     5281 cattggcgcg tttgatgatt ttgtcgtcga tatcggtaat gttgcgcaca tacttcagtt
     5341 tatagccgag gaaacgcaga tagcgcgcaa ccacgtcaaa agcaacaaag gtacgcccgt
     5401 gaccgatatg acagagatcg taaacggtga ttccacacac gtacatgccg acttccccgg
     5461 cgtgaatagg cttaaattcc tctttttggc gtgtcagagt attgaagatt tttagcatcg
     5521 aagattccgt ttagacatgt gtgggtaatt gagttgcgta taatacccat atttcccgcg
     5581 cgaatcagca tacattgcgt gatgatccga tcgtgcggtt atgctataac accaccctat
     5641 atatgacccg aactgggttg aagcaccaat caaacggaac aggatgcaaa aatggttact
     5701 ttccacacca atcacggcga tattgtcatc aaaacttttg acaataaagc acctgaaaca
     5761 gttaaaaact tcctggacta ctgccgcgaa ggtttttaca acaacaccat tttccaccgt
     5821 gttatcaacg gctttatgat tcagggcggc ggttttgaac cgggcatgaa acaaaaagcc
     5881 accaaagaac cgatcaaaaa cgaagccaac aacggcctga aaaatacccg tggtacgctg
     5941 gcaatggcac gtactcaggc tccgcactct gcaactgcac agttcttcat caacgtggtt
     6001 gataacgact tcctgaactt ctctggcgaa agcctgcaag gttggggcta ctgcgtgttt
     6061 gctgaagtgg ttgacggcat ggacgtggta gacaaaatca aaggtgttgc aaccggtcgt
     6121 aacggtatgc accaagacgt gccaaaagaa gacgttatca ttgaaagcgt gaccgttagc
     6181 gagtaatcgt ggcgacactc tttattgcag atcttcatct ctgcgtggaa gaaccggcga
     6241 tcaccgccgg ttttctgcgt tttttagcgg gggaagcccg caaggccgac gcgctgtata
     6301 ttcttggcga tctgtttgaa gcatggattg gcgacgacga tcccaaccca ctccatcgcc
     6361 agatggcggc agcgatcaaa gcggtgtccg attccggcgt tccctgttat ttcattcatg
     6421 gcaaccgtga ttttctgctc ggcaaacgct ttgcccgtga aagcggcatg acattattgc
     6481 cggaagaaaa ggtgctcgaa ctttatggtc gccgggtgtt gattatgcat ggcgacacgc
     6541 tgtgcaccga tgacgcgggt tatcaggctt ttcgcgccaa agtccacaaa ccctggctgc
     6601 aaatgctatt cctcgccctg ccgttgtttg tgcgcaaacg cattgccgcg cgaatgcgcg
     6661 cgaacagcaa agaagccaac agcagtaaat cgctggcgat catggacgtt aaccaaaacg
     6721 ctgtggtcag tgcgatggaa aaacatcagg tgcaatggct aatccacggg catacccatc
     6781 gcccggcggt gcatgaactt atcgccaacc agcaacctgc ttttcgcgtg gtactgggtg
     6841 cctggcatac ggaaggttcg atggtgaaag tcacggcgga tgacgttgag ctgattcatt
     6901 ttccgtttta aaaaacccac aactttgctg atttcacagc cacgcaaccg ttttccttgc
     6961 tctctttccg tgctattctc tgtgccctct aaagccgaga gttgtgcacc acaggagttt
     7021 taagacgcat gtcttcccgc aataatccgg cgcgtgtcgc catcgtgatg gggtccaaaa
     7081 gcgactgggc taccatgcag ttcgccgtcg aaatcttcga aatcctgaat gtcccgcacc
     7141 acgttgaagt ggtttctgct caccgcaccc ccgataaact gttcagcttc gccgaaagcg
     7201 ccgaagagaa cggttatcag gtgattattg cgggcgcagg cggcgcagcg cacctgccag
     7261 gcatgattgc cgccaaaacg ctggtgccgg tgctgggcgt gccagtacag agcgccgcac
     7321 tgagcggtgt cgatagcctc tactccatcg tacaaatgcc gcgcggcatt ccggtgggta
     7381 cgctggcgat tggtaaagct ggcgcggcaa acgcggcatt actggcagca caaattctcg
     7441 cgactcacga taaagagcta caccagcgtc tgaatggctg gcgcaaagcc cagaccgacg
     7501 aagtgctgga aaacccggac ccgcgaggtg cggcatgaaa caggtttgcg tcctcggtaa
     7561 cgggcagtta ggccgtatgc tgcgtcaggc aggcgaaccg ttaggcattg ctgtctggcc
     7621 ggtcgggctg gacgctgaac cggcggcggt gccttttcaa caaagcgtga ttaccgctga
     7681 gatcgaacgc tggccggaaa ccgcattaac ccgcgagctg gcgcgtcatc cggcctttgt
     7741 gaaccgcgat gtgttcccga ttattgctga ccgtctgact cagaaacagc ttttcgataa
     7801 gctccacctg ccgaccgcgc cgtggcagtt acttgccgat cgcagcgagt ggcctgcggt
     7861 gtttgatcgt ttaggtgaac tggcgattgt taagcgtcgc actggtggct atgacggtcg
     7921 cggtcaatgg agtttacgcg ccaatgaaac cgaacagtta ccggcagagt gttacggtga
     7981 atgtattgtc gagcagggca ttaacttctc tggtgaagta tcgctggttg gcgcgcgcgg
     8041 ctttgatggc agcaccgtgt tttatccgct gacgcataac ctgcatcagg acggtatttt
     8101 gcgcaccagc gtcgcttttc cgcaggccaa cgcacagcag caggcgcaag ccgaagagat
     8161 gctgtcggcg attatgcagg agctgggcta tgtgggcgtg atggcgatgg agtgttttgt
     8221 caccccgcaa ggtctgctga tcaacgaact ggctccgcgt gtgcataaca gcggtcactg
     8281 gacacaaaac ggtgccagca tcagccagtt tgagctgcat ctgcgggcga ttaccgatct
     8341 gccgttaccg caaccagtag tgaataatcc gtcggtgatg atcaatctga ttggtagcga
     8401 tgtgaattat gactggctga aattgccgct ggtgcatctg cactggtacg acaaagaagt
     8461 ccgtccgggg cgtaaagtgg ggcatctgaa tttgaccgac agcgacacat cgcgtctgac
     8521 tgcgacgctg gaagccttaa tcccgctgct gccgccagag tatgccagcg gcgtgatttg
     8581 ggcgcagagt aagttcagtt aagcaggtgc tcatttgatt gccggatgcg gcgtaaacgc
     8641 cttatccggc ttaccgctcc ggtacccatt gtaggcctga taagacgcgt caagcgtcgc
     8701 atcaggcatg gtgcaccgat tgccggatgc tctcatcact acagcgaaat acaggtcccc
     8761 gcttcgcccg ccagcgtctc ttcaattcgc gataacgccc caatccacgc gggtttacta
     8821 cggcttctga cataaccact caccgccgtt accttcggcc ccatcgaacc atcggctttg
     8881 gcaaatggcg ctaactcatc cggtgtggca tggcgaatgg cacgttgctg cggcgttccc
     8941 cagttttcat ataccgcatc agcatcggtg aggatcacca gtccatcggc attaatctgc
     9001 tcggcgagca acgcagcggc gagatcttta tcaatcaccg cttcactccc tgccccatct
     9061 tccgtcacag gcacaccgcc gccgccactg caaatcacca catgcccctc tttgagcaac
     9121 aactcgatgg cttcgctgtc gagaatttta cgcggttgcg gagacgccac cacccggcgc
     9181 aaatatttac catcacgttt catctgccag ccgtaagccg cttccagtgc ctcttgttct
     9241 tctggctgat aaaccggacc aataaatttc tctggctgca aaaacgccgg atcatcaggc
     9301 gaaacctcaa tgcgcgtcag caccgtcgta acaggcggca tctgcggctg tgcgctcaag
     9361 ctctgcgcca gcatatagcc aatcatcccc tggctttccg ccaccagcac atccagcgga
     9421 tacggttcta cctctttcca cgccagattc tgtaacgcca gcagccccac ctgcggcccg
     9481 ttgccgtgaa caatcgccag ccgataagaa cgggccaggc gtgccagcgc gggtacagca
     9541 ctggcgatat tgcgatattg attttccgcc gtcagcgcct caccgcgctg gagtaaggcg
     9601 ttgcccccaa gagctacaac cagtgttttc atggttttcc ttgtaataat tgttggccaa
     9661 gccagaaccc cagcagcgta tccgcccctg acgtatgccc cagcgccagc agcgaatcaa
     9721 tcgcaacagc ggtacgtttc gggcaactca gagcatggac aaagtgcagg agtggcgagg
     9781 cgaaatatcc ttgcgcggca taacgtaaat aactgacgct caccgctgtg gtaacgagtt
     9841 gaagattgtc ggaacaggca aaaaacgggc gaccggcgcg cgcatctaaa gcgccataat
     9901 accaggccgc cagcagcatt ccgctcaacg tgtcatcatg acttggcgtt aatccggggc
     9961 ctttacccag ccagtgccgc cagtcggtct taacgccatt gagcgcggcc tgaaaacagt
    10021 gacgaaactg gcgtaattca gcgggcagcg gatcgcttgc cgccatcgcc agtggcccga
    10081 aaagtccggt ttcctccgcg cgttgcatcc atgcagctgc aagcggttga ggatgcgaag
    10141 gcggcgtaat acgcagcaaa caataacgct gtggctgttt aaccgtgaaa cgcccgaggc
    10201 gaatcccttg agccacaacc tgtggtcgtt cattgccgca taatccaccg cataacgcat
    10261 cgaattgcgt gcggcgaaat tacacattcc cgtcaagcta tagaagaaac gctgccctgg
    10321 ctttcctctt ttggcgctga cccaacgggt gggatgaccc gtttacttta ttcgccggaa
    10381 tggctggaaa cccagcagca atttaaaaaa agaatggcag caagtgggct ggaaacacgt
    10441 ttcgatgaag tggggaattt atacggtcgc ctgagtggca ccgaatatcc acaggaagtg
    10501 gttctgagcg gttcgcatat cgataccgtg gttaacggcg gtaacctcga cgggcaattc
    10561 ggcgcgctgg cggcgtggct ggcaattgac tggttgaaaa cgcaatacgg cgcaccgtta
    10621 cgtacggtcg aagtggtggc gatggcagaa gaagaaggca gccgcttccc gtatgtcttc
    10681 tggggcagta aaaatatctt tgggctggcg aatcctgacg acgtgcggaa tatctgtgat
    10741 gccaaaggaa atagttttgc cgatgcgatg aaggcttgcg gatttactct gcctaacgcc
    10801 ccactaactc cgcgtcagga tataaaagcc tttgttgaac tgcatattga acagggctgt
    10861 gtgctggaaa gtaatgggca atcaattggc gtggtgaatg caattgtcgg gcagcgtcgc
    10921 tatacggtga cgctgaacgg cgaatcaaac catgcaggca ccacgccgat gggttatcgt
    10981 cgtgatacgg tttacgcttt cagtcgcatt tgccatcagt cgatcgaaaa agcgaaaaag
    11041 atgggcgatc cgctggttct gacctttggc aaagtagagc cgcgcccgaa tacggtaaat
    11101 gtggtgccgg gtaaaaccac gttcaccatt gattgtcgtc ataccgacgc tgccgtgctg
    11161 cgcgatttca cccaacagtt agaaaacgac atgcgggcga tttgcgatga aatggacatt
    11221 ggtattgata tcgatttatg gatggacgaa gaacccgtgc cgatgaataa ggagctggtc
    11281 gccaccctga cagaattgtg tgaaagagaa aaactgaatt accgggtgat gcacagtggt
    11341 gccgggcacg acgcgcaaat tttcgcgcct cgcgtaccaa cctgcatgat ttttatcccc
    11401 agcatcaacg ggatcagcca taacccggcg gaacgcacca atattactga ccttgccgaa
    11461 ggggtcaaaa cgttggcact catgctttat caacttgcct ggcagaaata aggagtcata
    11521 aatgggatat ttaaataacg tcaccggtta ccgcgaagat ttactggcta accgtgcgat
    11581 tgttaaacac ggtaatttcg cactgttaac cccggatggt ctggtaaaaa atattattcc
    11641 gggctttgaa aattgtgacg cgacaatcct ctccacgcca aagctgggtg cctcttttgt
    11701 tgattatctg gtcacactgc atcaaaacgg tggcaaccaa cagggcttcg gtggcgaagg
    11761 cattgaaacg ttcctgtatg tgatctctgg aaatatcact gccaaagccg aaggcaaaac
    11821 atttgcctta agcgaaggtg gctatcttta ttgcccacca ggctccttaa tgacgtttgt
    11881 taacgcccag gccgaagaca gccaaatctt tttatataag cgccgctatg ttccggcaga
    11941 aggccatgca ccgtggctgg tttctggcaa tgccagcgaa ctggaacgca ttcactatga
    12001 aggtatggat gatgtgatcc tgctggattt tctgcccaaa gagttaggtt ttgatatgaa
    12061 catgcatatc ctctcttttg caccaggtgc cagccacggt tatatcgaaa cacacgttca
    12121 ggaacatggt gcctatattc tttccggtca gggggtttat aacctcgaca ataactg
//
LOCUS       DAJTYG010000103        12154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTYG010000103 DAJTYG010000000
VERSION     DAJTYG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.103"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            128..997
                     /locus_tag="OO568_003225"
     CDS             128..997
                     /locus_tag="OO568_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR6642470.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1200..1553)
                     /locus_tag="OO568_003226"
     CDS             complement(1200..1553)
                     /locus_tag="OO568_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6642471.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1691..3337)
                     /gene="groL"
                     /locus_tag="OO568_003227"
     CDS             complement(1691..3337)
                     /gene="groL"
                     /locus_tag="OO568_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6642472.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3381..3674)
                     /locus_tag="OO568_003228"
     CDS             complement(3381..3674)
                     /locus_tag="OO568_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6642473.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3950..5206
                     /gene="yjeH"
                     /locus_tag="OO568_003229"
     CDS             3950..5206
                     /gene="yjeH"
                     /locus_tag="OO568_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6642474.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLEALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="OO568_003230"
     CDS             complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="OO568_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6642475.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6035..7471
                     /gene="aspA"
                     /locus_tag="OO568_003231"
     CDS             6035..7471
                     /gene="aspA"
                     /locus_tag="OO568_003231"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6642476.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7589..8890
                     /gene="dcuA"
                     /locus_tag="OO568_003232"
     CDS             7589..8890
                     /gene="dcuA"
                     /locus_tag="OO568_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6642477.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9006..9344
                     /gene="cutA"
                     /locus_tag="OO568_003233"
     CDS             9006..9344
                     /gene="cutA"
                     /locus_tag="OO568_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6642478.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9320..11017
                     /gene="dsbD"
                     /locus_tag="OO568_003234"
     CDS             9320..11017
                     /gene="dsbD"
                     /locus_tag="OO568_003234"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6642479.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTIDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11054..11629
                     /locus_tag="OO568_003235"
     CDS             11054..11629
                     /locus_tag="OO568_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6642480.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11736..11811
                     /locus_tag="OO568_003236"
     tRNA            11736..11811
                     /locus_tag="OO568_003236"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11769..11771,aa:Phe,seq:gaa)
BASE COUNT         2927 a         3133 c         2987 g         3107 t
ORIGIN      
        1 ttttctgttt atatagagtg agcacggcta tttatgttat tttaacaact attactacca
       61 agcccattta tctggcttat aaattaaaat taattattta attgagaaat ggttagggag
      121 aacctacatg gccataagca tcaagggtgt taataccggt gttattcgca agagtaataa
      181 tttcattgca ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat
      241 gtctgtcatg gaactgcgcg acctgctgat tgcgctggaa agtcgtctgc atcaaaagca
      301 taaactggat gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa
      361 aatggcagaa aacatcccag aaattctggt tgatgagctt aaaaatgccg atattaatcg
      421 tcgggttaac acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac
      481 gctacatgac ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctggc
      541 gcgagcaatc attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac
      601 ctcactgtta gatttcctgc cgttatacga cgttgactgc caggacaatg gcaatctgga
      661 atatgacacc tattcccaac cggaatggaa acataacttg ttcgaccatt atctggcggt
      721 gctttaccgc tttaaagatg aaagtggcaa agaacaattt agcggcgcag tggtgaaaac
      781 gcgtgaagca acgccgggta aagaaataga ggcgatcact cgccgaatgc ttgatttcag
      841 cccacgattg aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca ccgtagcggc
      901 gaataacgcc caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt
      961 acaatccacc agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc
     1021 tggagccatc cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcggcgt
     1081 aaacgcctta tccggcctac aaattctcaa gcattcaatc agttgcaatg cctgctgtag
     1141 gcctgataag cgaagcgcat caggcaataa tacgtttgtc acccagcctc tggatcacat
     1201 cagttcgggc acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac
     1261 aacattccct gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc
     1321 tggttgcgca gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg
     1381 gaaagccagt tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc
     1441 tgctcgtcca caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa
     1501 cccgccatca gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc
     1561 cccataatca atgagctgga cgccactcgc gtcgtccgtg tctgaatctt atactagaaa
     1621 gatgaccaaa agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgaag
     1681 tgcagggcaa ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc
     1741 ctaagtcagc tgcatcgttt ttcggcaggt cggtaaccat gcattcggtg gtgatcatca
     1801 ggccagccac agaagctgcg tactgcagag cagaacgggt tactttggtt gggtccagga
     1861 tacccatgtc gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt
     1921 cgccgccttt aacggtgtta gcaacaacag acggttcttc gccgcagttc aggacgatct
     1981 gacgcagcgg agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt
     2041 tctgaccacg caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag
     2101 caaccacgcc ttcttctacc gcagcacggg tcgcgtgcag ggcatcttca acgcgtgctt
     2161 ttttctcttt catttcaact tcggtagcag cacctacttt gataactgca acgccgcctg
     2221 ccagtttcgc tacgcgctcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa
     2281 tctgctgacg gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcga
     2341 tgatagtggt ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg
     2401 ttgctttttc cagctccata ccgatctctt cagagattac ggtaccgcca gtcagggttg
     2461 cgatatcctg cagcatagct ttacgacgat cgccgaagcc cggagcttta accgcagcaa
     2521 ctttcacgat gccacgcatg gtgttaacaa ccagagttgc tagcgcttcg ccttcaacat
     2581 cttcagcgat gatcagcagc ggtttgcctg ctttggcaac agcttccaga accggcagca
     2641 tttcgcggat gttggagatt ttcttgtcag ccagcaggat gaacgggctt tccagttcta
     2701 ctgcgccagt ttccggcttg ttgatgaagt aaggagacag gtagccacgg tcgaactgca
     2761 taccttcaac cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc
     2821 cttctttacc gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt
     2881 tagcggagat ggtaccaacc tgagcaatcg ctttagaatc agagcacggt acggacagcg
     2941 ctttcagttc ttcaactgca gcggtaaccg ctttgtcgat accacgtttc aggtccatcg
     3001 ggttcatgcc cgcagcaaca gctttcagac cttcagtgat gatagcctga gccagtacgg
     3061 ttgcagtggt ggtaccgtcg cctgcagcgt cgttcgcttt ggaggcaact tctttcacca
     3121 tctgcgcacc catattttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac
     3181 catctttggt gatggtcggt gcaccgaaag atttatccag aactacgtta cggccttttg
     3241 gaccgagggt aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac
     3301 gagcgtcgtt accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta
     3361 tgttcagtgt cgtgcgcgga ttacgcttca acaattgcca gaatgtcgct ttcggacatg
     3421 atcaacactt cttcattgtc gatcttctcg gatttcacac cgtagccatc gttgaaaata
     3481 acgatgtcgc caactttcac gtccagcggc ttcacttcgc cattttcaag gatacggcca
     3541 ttgccgacag ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg
     3601 atgccgccag cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat
     3661 ggacgaatat tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc
     3721 ggagcttacg tggtttcccg gctggtgacc agagaaatgg ggatgaggct tcgccccttc
     3781 aagggggaaa aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac cctgattcac
     3841 ctcacatttc atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc
     3901 gaaaatctac gcagttgccc aaaatttggg cgcaatcgga tatcaaccca tgagtggact
     3961 caaacaagaa ctggggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac
     4021 tggcgtgttt gccgttcctg cgttagctgc actggtagca ggcaataaca gcctgtgggc
     4081 gtggcccgtt ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg
     4141 ccactatccc agcgcaggcg gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct
     4201 tgagcgagtc accggctggc tgtttttatc ggtcattccc gtgggtttgc ctgccgcact
     4261 acaaattgcc gccggattcg gccaggcaat gtttggctgg catagctggc aactgttgtt
     4321 ggcagaactc ggtacgctgg cgttggtgtg gtatatcggt actctcggtg ccagttccag
     4381 tgctaatcta caaaccgtta ttgccggact tatcgtcgca ctgattgtcg ctatctggtg
     4441 ggcgggcgat atcaaacctg cgagtatccc cttccccgcg ccaggaaata tcgaacttac
     4501 cgggttattt gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc
     4561 ccatcttgcc tcggaattta aaaatccaga gcgtgatttt cctcgtgctt tgatgattgg
     4621 tctgctgctg gcaggattag tctactgggg ctgtacggta gtcgtcttac acttcgacgc
     4681 ctatggtgaa caaatggcgg cggcagcatc gcttccaaaa attgtagtgc agttgttcgg
     4741 tgtaggagcg ttatggattg cctgcgtaat tggctatctg gcctgctttg ccagtcttaa
     4801 catttatata cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca
     4861 ctacctggca cgcctctctt ctcgccatat cccgaataat gccctcaatg cggtgcttgg
     4921 ctgctgcgtg gtgagcacgt tggtaattca tgccttagag atcaatctgg aggctcttat
     4981 tatttatgcc aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa
     5041 attattgcaa ggtcgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt
     5101 actggcaatg gtcggctgga aaagtctcta tgcgctgatc atgctggtgg ggttatggct
     5161 gtttctgcca aaacgaaaaa caccggaaaa tggcataacc acataatccg gcgtttcgac
     5221 attaatcctg gcgatcgtct ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctgat
     5281 actcaccatc aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc
     5341 gagaaaagcg caaatgtggc atcaacttca ctgtcagatg cttttgcacc ggcggcaata
     5401 aaagcagaag accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa
     5461 taatcagcga aacactttta atcatctccg ccgcagggtt ttcacccgcc gccatttttt
     5521 gctgcatcag cacgaaattc ttaaagccct ggttacgtac cagtgacata ccgataactg
     5581 acgtgaatat aaccagcacg agggtcagca atacccccaa tacatgggca acctgaataa
     5641 agattgaaat ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag
     5701 gtttctcctg taatagcagc cggttaaacc cggctacctg aatgggttgc gaatcgcgtt
     5761 cagcttatat tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt
     5821 aatcccaaag cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta
     5881 aagtgatcca gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga
     5941 tgcaggggat aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa
     6001 gcaaatcatt ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga
     6061 agatctgttg ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag
     6121 agcgattgaa aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg
     6181 cggtatggta atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc
     6241 taaaagtgta gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg
     6301 catggatcag ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa
     6361 caccaacgaa gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata
     6421 tcagtacctg aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc
     6481 gaccggtttc cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca
     6541 actgcgtgaa ggctttgaac gtaaggctgt cgaattccag gacatcctga aaatgggtcg
     6601 tacccagctg caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat
     6661 cctgctgaaa gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa
     6721 ccttggcgca acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc
     6781 agtgaaaaaa ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga
     6841 agcgacctct gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt
     6901 gaagatgtcc aaaatctgta acgacctgcg cttgctctct tctggtccac gtgccggcct
     6961 gaacgagatc aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa
     7021 cccggttgtt ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac
     7081 tgttaccatg gcagcagaag caggtcagct gcagttgaac gttatggaac cggtcattgg
     7141 ccaggccatg ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa
     7201 atgcattaac ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat
     7261 cggtatcgtt acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa
     7321 aatctgtgcc gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac
     7381 tgaagcggaa cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc
     7441 aaaacgctat actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaaaaaggc
     7501 acgttagatg acgtgccttt tttcttgtga gcagtaactt aaaaataaca atataatatc
     7561 aacttgttaa aaaacaagga aggctaatat gctagttgta gaactcatca tagttttgct
     7621 ggcgatcttc ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg
     7681 ggtgctggtt cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc
     7741 cattatcatg gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta
     7801 tctggttcat cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc
     7861 accgatcgtg acctatttcc tgactatctt tgctggtact ggcaacatct ctctggcgac
     7921 actgccagtt atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctctc
     7981 tactgcagtg gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt
     8041 ttacatgtct tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt
     8101 catcccgtcc accctgctgg cggttctggt gatgtccttc ctggtcacta tgctgttcaa
     8161 ctccaaactc tctgacgatc cgatttatcg caagcgtctg gaagagggct tggttgaact
     8221 gcgcggtgaa aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct
     8281 gctgggcgta gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt
     8341 tgaaaaaccg ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac
     8401 tctgaccacc gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa
     8461 agcaggtatg agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt
     8521 ttccaacaac atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg
     8581 gctgctggcc gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc
     8641 aaaagcactg atgccgatgg ctctggcact gaacgtttca ccgctgaccg ctgttgcttc
     8701 tttcgctgcg gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt
     8761 acagatggat gacacgggta ctacccgtat cggtaaattc gtcttcaacc atccgttctt
     8821 catcccgggt actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt
     8881 catgctgtaa tgatcaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca
     8941 tccttccccc gtccgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg
     9001 tgtttatgct tgatgaaaaa agttcgaata ccgcgtctgt cgtggtgcta tgtaccgcac
     9061 cggatgaagc gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct
     9121 gcgcgacctt gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag
     9181 aatacgaagt gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat
     9241 gcctgaagtc tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg
     9301 gagacacaga ttacctctca tggctcaacg catctttacg ctgattctgc tactttgcag
     9361 cacttccgtt tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga
     9421 tcaagccttt gcttttgatt ttcagcaaaa ccaacatgac cttaatctga cctggcagat
     9481 caaagacggt tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat
     9541 tgccgacgtg cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga
     9601 gatttaccgc gatcggctga cgcttcccgt aactatcaac caggcgagtg cgggagcgac
     9661 gttaactgtc acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa
     9721 aaccgttccg ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgtttc
     9781 gcagcaagag cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg
     9841 tattggtatc gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt
     9901 gctgggtggt aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt
     9961 gcaggggatg gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca
    10021 gttccaggcg gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt
    10081 gctggcgatg tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacacg
    10141 tctcacgttg atgagcaatc gccaacaggg cggctcacct ggcggtgtgt ttgttatggg
    10201 ggcgattgcc ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgct
    10261 gtatatcgcc caaagcggga acatgtggct gggcggcggc acgctttatc tctatgcgtt
    10321 gggcatgggc ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag
    10381 cggcccgtgg atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt
    10441 cttcctgctg gagcgagtga ttggtgatgt atggggatta cgcttgtggt cggcgctggg
    10501 tgtcgcattc tttggctggg cctttatcac cagcctacag gctaaacgcg gctggatgcg
    10561 cgtggtgcaa ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg
    10621 ggcatttggt gcgacgcata ccgcgcaaac tcagacgcat ctcaacttta cacaaatcaa
    10681 aacgatagat gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga
    10741 tctttatgcc gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc
    10801 gcaggtgcaa aaagcgttag cagacacggt cttacttcag gccaacgtca cggccaacga
    10861 cgcacaagat gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt
    10921 ttttgacgga caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga
    10981 aaccttcagc gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac
    11041 ggaggaaaat agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt
    11101 acaagggatt gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga
    11161 cgagctacgc cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct
    11221 tagccaacag atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga
    11281 acaaaagctg ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc
    11341 gggctgtcta tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctattcatca
    11401 actggccgat cagcaaaaaa gcgcggccta cgatttcacc cacgaactgt taaccacgct
    11461 ggaagttgac gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt
    11521 aagccgaatg ctggtgaatc gtagccaggc ggatgtcgac actgcacatc ggctggcgga
    11581 agatatcctg cgcttcgccc gctgccgcca gggtggtgca ttgacctgac agaaacacag
    11641 aaaagaagcg atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga
    11701 cgagatgtgc agattacggt ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc
    11761 aggggattga aaatccccgt gtccttggtt cgattccgag tccgggcacc aaattcatat
    11821 caacggacct ccacggaggt ccgtttttcg tttcaggacg ccacgattta agcgttctgc
    11881 ctccaaatca attctaccga actcaaccag attctcccca catcaccagc aatttgcgag
    11941 catatcccaa tttgggaaaa tttgttcctg agctatagca ctaactgacg tgaaatgtcg
    12001 tgcggccccg tgatgctgtt gaacgtcaaa tgacgtcatc aggagcgtaa cgtatccata
    12061 aagcacaaca ccggtctgaa cgccgactga tgagattttc tgaatgagaa caaagagaaa
    12121 tgtgtcagtc cgcttgctca tgcaaagact aaca
//
LOCUS       DAJTYG010000104        11789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTYG010000104 DAJTYG010000000
VERSION     DAJTYG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.104"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            203..538
                     /locus_tag="OO568_003237"
     CDS             203..538
                     /locus_tag="OO568_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6642481.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            631..714
                     /locus_tag="OO568_003238"
     CDS             631..714
                     /locus_tag="OO568_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642482.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            814..1635
                     /locus_tag="OO568_003239"
     CDS             814..1635
                     /locus_tag="OO568_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6642483.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1674..2003)
                     /gene="mdtI"
                     /locus_tag="OO568_003240"
     CDS             complement(1674..2003)
                     /gene="mdtI"
                     /locus_tag="OO568_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6642484.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1990..2355)
                     /gene="mdtJ"
                     /locus_tag="OO568_003241"
     CDS             complement(1990..2355)
                     /gene="mdtJ"
                     /locus_tag="OO568_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6642485.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2766..3800
                     /gene="tqsA"
                     /locus_tag="OO568_003242"
     CDS             2766..3800
                     /gene="tqsA"
                     /locus_tag="OO568_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6642486.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMEMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVHFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3825..5213)
                     /gene="pntB"
                     /locus_tag="OO568_003243"
     CDS             complement(3825..5213)
                     /gene="pntB"
                     /locus_tag="OO568_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6642487.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGGVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARSINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5224..6756)
                     /gene="pntA"
                     /locus_tag="OO568_003244"
     CDS             complement(5224..6756)
                     /gene="pntA"
                     /locus_tag="OO568_003244"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6642488.1"
                     /translation="MQIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFNE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7280..8224
                     /locus_tag="OO568_003245"
     CDS             7280..8224
                     /locus_tag="OO568_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6642489.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            8410..9792
                     /locus_tag="OO568_003246"
     CDS             8410..9792
                     /locus_tag="OO568_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6642490.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQDAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9829..10551
                     /gene="folM"
                     /locus_tag="OO568_003247"
     CDS             9829..10551
                     /gene="folM"
                     /locus_tag="OO568_003247"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6642491.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10548..10883)
                     /locus_tag="OO568_003248"
     CDS             complement(10548..10883)
                     /locus_tag="OO568_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6642492.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            11012..11755
                     /gene="rstA"
                     /locus_tag="OO568_003249"
     CDS             11012..11755
                     /gene="rstA"
                     /locus_tag="OO568_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6642493.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
BASE COUNT         2996 a         3013 c         2835 g         2945 t
ORIGIN      
        1 cgctttgtaa gtagtataat agttaacccg atcaagacta ctattattgg tagctaaatt
       61 ttccttaagt cacaatacgt tattatcaac gctgtaattt attcagcgtt tgtacatatc
      121 gttacacgct gaaaccaacc actcacggaa gtctgccatt cccagggata tagttatttc
      181 aacggccccg cagtggggtt aaatgaaaaa acaaattgag ggtatgacaa tgaaaaaagt
      241 attagctctg gttgttgccg ctgctatggg tctgtcttct gccgcctttg ctgcagagac
      301 tgcgaccaca cctgctccga ctgcgacgac caccaaagca gcgccagcga aaactacaca
      361 tcataaaaaa cagcataaag cagcacctgc ccagaaagcg caggcggcta aaaagcatca
      421 taaaaatacg aaagctgaac agaaagcccc tgaacaaaaa gcgcaggcag cgaagaaaca
      481 cgccgggaaa cacggccatc agcaaccggc aaaacctgct gcacaacccg cagcgtaagt
      541 tttcaacagt aatgctggcg cgccccctcg cgcctgaaaa ttacggtgct aagcgggtaa
      601 cgtttagcac cgcctttagc ggagggtagt atggtgggcc gttatcgctt tgagttcatt
      661 cttatcatcc ttattttatg cgcactgatt gccgcccgtt tttatctttc ctgactgtag
      721 ttatctgatt ttactcccac tttcatcccg tcccgtctat agtatttatg agggtttgct
      781 tttaataatc ataattaccc accagagtgt gatatgcgta caaccattgc tgtagtgttg
      841 ggtgcaatta gtttgacgtc tgcttttgtg tttgcagata aaccagacgt tgccaaatcg
      901 gcaaacgatg aggtcagcac cctgtttttt ggtcatgatg atcgtgtgcc agtgaatgac
      961 acgacccaat caccgtggga tgcggttggg caactggaaa cggccagcgg caatttatgt
     1021 acggcgacgc tgattgcacc caatctggca ttaacggcag gacactgttt attgacgccg
     1081 cctaagggta aagcggataa ggcggtagcg ctgcgttttg tgtcaaataa aggtctttgg
     1141 cgctatgaga tccacgacat agaaggccgc gttgatccga cactgggaaa gcggttaaaa
     1201 gcagatgggg atggttggat tgttcctccc gcagctgcac cgtgggactt cggattgatt
     1261 gtgctacgta atcccccttc tggcattaca ccgttgccgt tatttgaggg agataaagcc
     1321 gcgcttactg ccgcattaaa atcggctggt cgtaaagtga ctcaggcggg ataccctgaa
     1381 gatcatctcg atacgttata cagtcatcaa aactgtgaag tgactggctg ggcgcaaacg
     1441 tcggtgatgt cacatcagtg cgataccttg ccgggtgaca gcggttcgcc tctgatgttg
     1501 cataccgatg acggctggca attaattggg gtgcaaagtt cggctcctgc cgcgaaagat
     1561 cgctggcgcg ccgataaccg ggccatttct gttaccggtt ttcgcgacaa gctggatcaa
     1621 ctgtcgcaaa aataatgttc aaacgctgcc cgacagcgcg ggcagcgtct tcatcaggca
     1681 agtttcacca tgatcattcc agccaacagc aagaccaggc caatccagcc tttacgattt
     1741 aaccgttgac caaacaagat ccaacctgcg gctaacgtgg cggcaatacc aaacccgccc
     1801 cacaatgcat aagcgacaga caagtcgatc cctttaacgg cctgagaaag cgcactaaag
     1861 gccgccagca ccgccgccag ggagagcaag ccaaatattt tgcgacgaaa gccgtcagaa
     1921 aatttcaaaa agacgttagc aacgatttcc agcacgattg ccaatgccag ccaggcggcg
     1981 tgaacccatt caaactgcgc catggttcac ctccagttca ggtttacgcg ctttacgggt
     2041 acctgatttg atcaacacta tcccggcgac cagggtggtt aacccggcaa ttttcatcag
     2101 cgataaactt tcgtcgaata acaaaacgct aaacaaggta ataaataaaa taccgatacc
     2161 ttcccacagc gcataagcta cgcctaaggc gattttttta acggcgaaag agagaaatat
     2221 atacgacaga gaaatcatca ccagcattaa aataaagccg ccatttccct cactgacgct
     2281 cgcccatttc attgacagcg taccggtaat ttctgtagca atagccagac ctaataaaat
     2341 ccaataaata tacattgtcc ttctcctgca agagaactat tttaattttc gcttaattca
     2401 gcgaaaccaa agtaaagtgg tcaagccctg cgcatagggc aagcttaggc agaagaaagg
     2461 actaaagcgc gttgcgccag tgctgctcac ctacgagcaa gatagtggat gaggtacgaa
     2521 gatgggtaaa tgtagaaaat aacgtcctga acaaattgtc cataatatta caattatccg
     2581 cagtgttgct tctcgtcatc gcagatgata attgtcctcg gtagttgaac acgcctgatt
     2641 tgtatcatag cttaagaatt aactcaaaat attttcactt ctttacctga gcggtttgat
     2701 tttcgttatg atgacggagc gaaaaagaca ttattattag caaaggaaga aaaacgggga
     2761 caagcatggc aaagccgatc atcacgctca atggcctaaa aatcgtcatt atgttgggaa
     2821 tgctggtcat tattctctgc ggtatccgtt ttgccgccga gatcatcgtg ccgtttattc
     2881 tcgcattatt tattgctgtt attcttaacc cgctggtgca acatatggtc cgctggcgtg
     2941 tgccgcgtgt actggcagtg tcgattttga tgacaatcat cgtgatggag atggtgttgc
     3001 tattagctta tctgggttcc gcgctcaacg agttgacgcg gacgttaccg caatatcgca
     3061 actctattat gacgccgctg caagctcttg agccgttgtt gcaacgcgta gggattgacg
     3121 tctcagttga ccaactggcg cattacattg atccgaacgc ggcgatgacg ttgctcacca
     3181 acttattgac gcagttatct aatgccatgt catcaatatt tttattgctg ctgacggtgc
     3241 tgtttatgct gctcgaagtg ccacaattgc ccggaaaatt tcagcaaatg atggcgcgtc
     3301 cggttgaagg gatggcggcg attcaacgtg cgattgacag tgtttctcat tatctggtgc
     3361 tgaaaacagc catcagcatc atcaccggct tggtcgcctg ggcgatgctc gccgcactcg
     3421 atgttcactt cgcttttgtc tggggattgc tggcctttgc gcttaattac atccctaata
     3481 ttggttcagt tctcgcggca atccccccta tcgctcaggt actggtgttt aatggcttct
     3541 acgaagcgtt gctggtgctg gcgggatatc tgctgattaa tctggtcttc ggcaatattc
     3601 tggagccgcg catcatggga cgtgggctgg ggctttcgac attggtggta tttttgtcgt
     3661 tgattttttg gggatggttg ttaggaccgg tgggtatgct gctttccgtg ccgttgacaa
     3721 ttattgtcaa aattgcgctt gaacaaacag cgggaggtca aagcatcgcc gttctgttaa
     3781 gcgatctcaa taaagagtga cggcctccgc agaggccgtc agggttacag agctttcagg
     3841 attgcatcca cgctggcttt ggcgtcacca aacagcatgt gggtgttttc cttgaagaac
     3901 agcgggtttt gcacaccagc atagccagtg ttcatcgaac gtttaaagac aatcacgttc
     3961 tgcgctttcc acacttccag cacaggcata ccagcaatcg gactcttcgg atcatcctgc
     4021 gccgccgggt taaccgtatc gttagcacca atcaccagta cggtatcggt atcagcaaag
     4081 tcatcgttga tctcgtccat ttccagcacg atgtcatacg gtacttttgc ttcagccagc
     4141 aatacgttca tatgtccagg caaacgcccc gcaaccgggt ggataccgaa acgcacatta
     4201 atactacgag cgcggagttt ctcggtaatt tcagcgacag gatattgcgc ctgcgcgact
     4261 gccatgccgt accccggagt aatgatcact gaatgggagt ttttcagcag ttccgctgtc
     4321 tcttctgcgg tgatttcgcg gtgctcaccc acttcctgat catcgccagt agaagagccg
     4381 tcggtgccga aaccacccgc aataacgctg ataaaggaac ggttcatcgc cttacacata
     4441 atgtaagaaa ggatagcccc cgaagaaccg accagcgcac cggtcacaat cagcaggtcg
     4501 ttgctgagca taaagcccgc agccgcagcc gcccagccgg agtacgagtt cagcatcgac
     4561 accaccaccg gcatatctgc accaccgatg gaggcgacta aatgccagcc gaataccagc
     4621 gcaatggcgg tcattatcag caatgccagc acttgcaggc cgacgctgtc cgtgcgaaca
     4681 aatacaatca gcagcaagaa ggaaacgacc agagccgcca ggttcatttt gtgacggttt
     4741 ggcagcatca acggtttaga cgaaatcttg ccacacagtt tgccgaacgc caccaccgag
     4801 cccgtgaacg ttaccccccc gatgaagata ccgaggaaca cttccgtcag gtgaatattg
     4861 accagaatcg gtgccattcc cgcgtcatga tgcagataac tgttaaagcc aaccagcact
     4921 gccgccagac ccacgaagct gtgcaggatc gccaccagtt ctggcatttc ggtcatttca
     4981 actttcttcg ccagccggat accaattgcc ccaccaatga ccatcgccag caagatccag
     5041 ccaacattac ccgtatccgg tccaaaaatg gttgcgatta acgcaatcgc catcccggcg
     5101 ataccgaagt tgttaccctg gcgagacgtt tcatgtttcg aaagaccggc cagactgaag
     5161 ataaacagga tcgcggcaac aatgtatgca gctgtaacta atcctccaga catatgttac
     5221 cccttaattt ttgcggaaca ttttcagcat gcgctgagtc acggtgaagc caccgaaaat
     5281 attaatgctg gctataagca ccgcgataaa actaaggaag ctaacccagc ctccctggcc
     5341 aatctgcaac agtgctccga caacaataat ccctgaaatc gcgttggtaa ccgacatcaa
     5401 cggtgtatgc agcgcgtgcg atacattcca caccacgtaa taaccgacaa cgcaggccag
     5461 cgcgaaaacg gtgaagtgcc caagaaactc tttcggcgca acgcttgaca gccaaccgaa
     5521 caggatgatt gccagcgcca tcaacgcgta tttacgccac ggtgagcagg cacatttttc
     5581 ctcagttttc acttccggtg ccgctttttg tgccgcctgc ggctgagctg atacctgaat
     5641 cggcggtgcc ggccaggtaa tttcgcccgc acggatcacg gtcacgccgc gaatcaccac
     5701 atcatcaaaa tcaacagtga tattgccgtc tttctctttg cacaacagtt tcagcagatt
     5761 aacgaggtta gtaccgtaaa gctgtgagga ttgcgtcggc agacggcccg gaagatcggt
     5821 ataaccaatc actttgacac cattttccgt actgaagatt tcacccggca cgatgtattc
     5881 acagttgcca ccgttttggg ctgccaggtc gacaatcaca ctgcccgcct tcatggagtc
     5941 aaccatttca cgggtaatta gcttcggcgc tggtttgcct ggaataagcg cggtggtgac
     6001 aatgatatcg acctcttttg cctgggcggc aaagagttcc atttccgctt tgatgaacgc
     6061 gtccgacatc actttggcat agccatcgcc gctgcccgct tcctcgttaa aatccagctc
     6121 gaggaattcc gcgcccatac tttgaacttg ttctttcact tccgggcggg tgtcgaatgc
     6181 acgcacaatc gcgccgagac tgtttgctgc gccaatggcg gccaaacctg caacaccggc
     6241 accaatcacc atcacttttg ccggtggcac tttcccggcc gcagtgattt gcccggtaaa
     6301 gaagcgccca aattcatgtg ccgcttcaac aatggcgcga taaccggcga tattcgccat
     6361 cgagcttagt gcgtccagcg attgtgcgcg tgagatacgc ggcacagagt ccatcgccat
     6421 cacggtcacg ttacgttccg caagtttttg cattaattcc ggattctgcg caggccagat
     6481 aaaactcacc agcgttgtcc cgggattcag taacgcaatt tcatcatcta acggcgcatt
     6541 gaccttcaga atgatctctg actgccagac gctattccct tctacaattt cagcgcccgc
     6601 ttgcacaaac gctttatcgt caaaacttgc cagttgaccc gcgccgctct ctaccgcgac
     6661 ggtaaaaccc agtttcagca gctgttccac tgtttttggc gttgctgcaa cacgggtttc
     6721 attggttaac cgttctcttg gtatgccaat ttgcatgata ttcccttcca tcggttttat
     6781 tgatgatggt ttgcctgtgt caggagccac acaagccgct catgtacgag ctaaatgtta
     6841 ctccgttaaa ataaattagt aacaaacgcc ctataacgta ctgaaaatta tgcctgtgat
     6901 ctagcgccaa aaatcagtat ttcggcgtga actcgcaaaa tattaacgat tcagccgtga
     6961 tagtgggata aacaccttag aacgccggat aaagactgat aattgtcttc gacggtcggg
     7021 taaaacgaga catcgccccg gcacgaatca ctacttaaca ttaaattaac ttatacaatt
     7081 cagttgcttc agtagtaatg atgctgatac ggctgttttt taagcataga cggtcatttg
     7141 agcaggatta aaattggttt aaggaatgtg atatgaaaaa tgacgcagac agttacaccg
     7201 tttaaatgca ataatcagcc acgtttctcg ttaataacaa taccagtacc tggtttgcgc
     7261 aaggcgaagg attattttta tgaagcttaa gaacaccctc ctggcgtcgg cactgctttc
     7321 tgctacggca ttctccgtta acgcagcaac agaactgaca ccggagcaag cggcagcggt
     7381 taaacctttt gaccgtgtag tggttaccgg tcgttttaat gctattggcg aagcggtgaa
     7441 agccgtttct cgtcgcgcag ataaagaagg tgccgcctct ttttatgttg tcgacacttc
     7501 tgattttggt aacagcggta actggcgtgt ggtcgctgac ctctataaag ccgatgctga
     7561 aaaagcagaa gaaacaagta atcgcgtaat taacggtgtt gtcgaactgc cgaaagatca
     7621 ggctgttctg attgaaccgt ttgacacggt caccgtccag ggcttctatc gtagccagcc
     7681 agaagtcaat gatgccatca ccaaagcggc aaaagcgaaa ggtgcctact ctttctacat
     7741 cgttcgtcaa atcgatgcca accagggcgg caaccagcgt attactgcat tcatctataa
     7801 aaaagatgct aagaaacgta tcgtccagag cccggatgtg atcccggcag attccgaagc
     7861 aggacgtgca gctctggctg ccggtggcga agccgcgaag aaagttgaga tcccgggtgt
     7921 tgcgactacc gcatcaccaa gttctgaagt cggtcgcttc tttgaaaccc agtcatcaaa
     7981 aggcgggcgt tacaccgtca cgctcccgga tggcactaaa gtcgaagaac tgaacaaagc
     8041 gacagcagcg atgatggtcc cgttcgacag catcaaattc tctggcaact acggcaacat
     8101 gaccgaagtc tcctatcagg ttgcgaaacg tgccgcgaag aaaggtgcta agtattacca
     8161 catcacccgc cagtggcaga aacgtggtaa taacctgacc gtcagcgcag atctgtataa
     8221 ataactattc agttactcct ataaggcggc ttgatgccgc ctttcgcatt tttagtcaca
     8281 aatttccccg cgaccattgc atgcagacac atctcttcgt aaaatcccgc gcttcagtgg
     8341 caaactccat ttttatgcgt tttgccctaa tatttattct tcttatcacg ttttaatcac
     8401 tggatatcga tggaaaagaa actgggactg agcgcactca ccgcgctggt attaagctca
     8461 atgctgggcg cgggtgtttt cagtctgccg caaaatatgg cggcagttgc cagcccggca
     8521 gcactgctca tcggctgggg tattactggc gctggcattt tattgctggc ctttgccatg
     8581 ctgatcctca cgcgcattcg tcccgaactt gatggcggta tctttaccta tgcccgcgaa
     8641 gggtttggcg agctaatcgg cttttgttcc gcatggggat actggctgtg cgcggtcatc
     8701 gccaacgtct cctatctggt gatcgttttt tccgcgttaa gcttttttac ggacacgccg
     8761 gaattgcgcc tgtttggcga tggtaatacc tggcaatcga tagtcggtgc atcggcttta
     8821 ttgtggattg ttcacttttt gatcctgcgc ggggtgcaaa ccgctgccag cattaacctg
     8881 gtggcgacac tggcaaaatt gttgccgctg ggtctgtttg ttgtgctggc gatgatgatg
     8941 ttcaaactgg ataccttcaa gctcgacttc accggacttg cacttggcgt acctgtttgg
     9001 gaacaggtga aaaacaccat gctgatcacc ctgtgggttt tcattggtgt ggaaggtgcc
     9061 gtggttgttt ctgcgcgtgc gcgtaataaa cgtgatgttg gcaaagcgac actgctggcg
     9121 gttctctccg ctctgggcgt ttacttgctg gtaacgctgc tttcgctggg tgtggtggca
     9181 cgtcctgaac tggctgaaat tcgtaacccg tcaatggccg gtctgatggt ggaaatgatg
     9241 ggaccatggg gcgaaatcat catcgctgcc ggtttgatcg tttccgtttg cggcgcgtac
     9301 ttaagctgga ccatcatggc ggcagaagtt ccgttcctgg ctgccactca taaagcattc
     9361 ccacgcattt ttgcgcgcca ggacgcacaa gctgcgccat ccgcctcact gtggttgacc
     9421 aacatctgcg tgcaaatttg tctggtactc atctggctga ccggttccga ttacaacacg
     9481 ttgctgacca tcgcctcaga aatgattctg gtgccctatt tcctggtcgg cgcgttcttg
     9541 ctgaaaatcg caacccgtcc actgcataaa gcggtaggtg tcggtgcctg catttatggc
     9601 ttatggttat tgtacgcctc tggtccaatg cacttgctgt tatccgttgt tctgtatgca
     9661 ccggggctgc tggttttcct gtatgcgcgc aaaacgcata cacatgataa tgtactgaac
     9721 cgtcaggaaa tggtgctgat tggcatgctg ttaattgcct cagttccggc gacctggatg
     9781 ctggtgggat aacgttcatc ccatcgttgg atagcaaagg agataacgat gggtaaaacc
     9841 cagcccttgc caatattaat tactggcgga ggtcgtcgca tcggcctcgc cctcgcatgg
     9901 catttcatta atcaaaagca accggtgatt gtcagctatc ggacacacta tccagccatt
     9961 gatggactga ttaatgcagg tgcgcagtgt attcaggctg atttttcgac caacgacggt
    10021 gtgatggcgt ttgccgatga agtactaaaa accacccatg gtctgcgtgc tattttgcat
    10081 aacgccagtg cgtggattgc ggaaaaaccg ggtgcgccac tggccgacgt actggcttgc
    10141 atgatgcaga tccacgttaa taccccatac ctgctcaacc atgcgctgga aagattactg
    10201 cgtgggcacg gacacgccgc cagcgatatc attcacttta ccgattatgt ggtggagcgc
    10261 ggtagcgaca aacatgttgc gtatgctgca agcaaagcgg cactggataa tatgacccgc
    10321 tcgtttgccc gcaagctggc accggaagtg aaagtgaatt ctattgcgcc atcgctgatc
    10381 ctgtttaatg aacatgatga tgccgaatat cgacaacagg cgctgaataa atcactgatg
    10441 aaaaccgcgc ctggcgagaa agaagtgatc gacctggtcg attacttact taccagttgc
    10501 tttgtcaccg gacgcagttt cccacttgat ggcggtcgtc atctgcgtta atgcagcttt
    10561 atccagcata taatcaacac ccatgcgctt atcccccaac acgcaacaga accaacaaaa
    10621 gcggcgggca agcgcatcat tccggtgaaa taccacagcg acaccaggta aacaaaatag
    10681 ggaataatcg accacatact aaaaatgatg gttgcgcgta aggcttcaat gccgcgttcg
    10741 ctggcaacaa tataatgcgc gataagcgca aaggtcggga aaagtggaat cagcccggcg
    10801 atataataat ttttcgtttt tgctaaaaca ccaatcaaca gcactaccag cgcaccgagc
    10861 gcggctttga ttaccagccc catcttttta ccttaacact tccataacaa gtcatcagta
    10921 gaatacctga tgaaaacttg tttagaaacg attgatagta agtaaaaaca gcgcggtgta
    10981 ttgtgacgtt tttatatcta ccgtgaatgt tatgaacact atcgtatttg tggaagatga
    11041 tgcggaagtc ggttcactga ttgccgcgta cctggcaaaa catgatatgc aggttaccgt
    11101 agaaccgcgc ggcgaccagg ccgaagaaac cattttgcga gaaaatccgg atttggtgtt
    11161 actcgacatc atgctaccag gcaaggacgg catgaccatt tgtcgtgatt tacgcgcaaa
    11221 gtggtctgga ccgattgttc ttctaacctc tctcgatagc gatatgaacc acatcctggc
    11281 actggaaatg ggtgcctgcg actatattct caaaacgacg ccccctgctg ttttgctagc
    11341 gcgtttacgt ttgcatttgc gtcagaatga gcaagccaca ctgaccaaag gtcttcagga
    11401 aacgtctctg actccctaca aagccctgca tttcggcacg ttaactatcg accccatcaa
    11461 ccgcgtagtc accctggcta acactgaaat ctcgctctcg acagctgatt tcgaattatt
    11521 gtgggaacta gctacgcatg ccgggcaaat catggaccgc gatgcattgc tgaaaaattt
    11581 acgcggcgtc agttatgacg gactggatcg tagcgtggac gtggctattt cgcggttaag
    11641 aaaaaaactg ctcgataacg ccgcagaacc ttatcgcatt aaaactgtgc gtaacaaagg
    11701 ctatcttttt gcgcctcatg catgggatag tgacaataaa caatcagatg actaatattt
    11761 gttcatctga aaaagagaac aattaacga
//
LOCUS       DAJTYG010000105        11739 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTYG010000105 DAJTYG010000000
VERSION     DAJTYG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.105"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..1176)
                     /locus_tag="OO568_003250"
     CDS             complement(<1..1176)
                     /locus_tag="OO568_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6642494.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRKLIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1375..1459)
                     /locus_tag="OO568_003251"
     tRNA            complement(1375..1459)
                     /locus_tag="OO568_003251"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1423..1425),aa:Leu,seq:caa)
     gene            1655..2674
                     /gene="ahr"
                     /locus_tag="OO568_003252"
     CDS             1655..2674
                     /gene="ahr"
                     /locus_tag="OO568_003252"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6642495.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2803..4317
                     /locus_tag="OO568_003253"
     CDS             2803..4317
                     /locus_tag="OO568_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6642496.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGEKKISQT"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="OO568_003254"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="OO568_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6642497.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFILVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKDDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="OO568_003255"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="OO568_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6642498.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="OO568_003256"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="OO568_003256"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6642499.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="OO568_003257"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="OO568_003257"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6642500.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..>11739
                     /gene="valS"
                     /locus_tag="OO568_003258"
     CDS             8962..>11739
                     /gene="valS"
                     /locus_tag="OO568_003258"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6642501.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAK"
BASE COUNT         2857 a         3089 c         3188 g         2605 t
ORIGIN      
        1 catcaaccta cgctccccaa gatgttccgc cttatggatg taagctgctc gcacagagct
       61 acgctcctgg tgactcatct gccgctctac cgcatccctc gaccataatc ccgactcaat
      121 caacgaacta caagccatcg tcctgaagcc atgcccacaa acctcgacct tcgtgtcata
      181 ccccattacg cgcaaagcct tgttcaccgt attctcactc atcggcttac gtggatcgtg
      241 atcgcccaca aaaatcagct ttcgatttcc gctcatgctt ttgatctttt ccaaaatact
      301 tagggcctgc cgcgacaaag gtacaaggtg tggtgtccgc atcttcgagc cacgctgaga
      361 atgcttgact ccttccagtt gctcacgctc acctgggatt gtccacatag ccgtttcaaa
      421 atctacttct gaccagcggg caaaacgcag ctcgctcgaa cggatgaaaa ccaataaagt
      481 gagttcaacc gccagtcgag ttaacggtct gccagaatag tgatctatgc gatgaagtaa
      541 ttcaggtata cggttcagtt caagagctgc acggtgctgt cttttcgccg tagcaaccgc
      601 acctgcaatc tcttgcgcgg ggttgtagtc gattaagccg ctctgaacgg caaagcgcat
      661 aatcgcggta gtacgctgct gtaaacgggc ggcaacttcg agccgcccgg atgactcgac
      721 tgctttaatg ggtacaagaa gatcccgcgt cttcagttcc gcaatgttcc gcttaccgat
      781 agcagtaaag agattatcct ccagactttt caaaacacga gcgctatgcg atgccgacca
      841 cttctgattg ctggcgtgcc agtctctggc gactacttca aacgttatcg cctcttgttc
      901 ctgttctacc ttaacagctt tcttgttttc actgggatcg acaccattag ctaaaagctt
      961 acgagcctca tcccggcgtg ccctggcatc cgccaacgac acttcggggt atttccccaa
     1021 tgcaagcatc ttctctttgc cgccaaagcg ataacgtagc cgccagtatt tggagccgtt
     1081 agggtgaacc agcaaaacca tgccttcacc gtcggtcagc ttataggctt ttgcttcagg
     1141 cttagccgaa cgaaccttca catcactcag agccatgatg agtatccttt caagggttct
     1201 gtgtgggtac aaacattatc gaaccgggaa atacccgcag ttgtacccgc atcagtaagt
     1261 tgatgtagat tgaatcaggt tgacttaggt tgagtgaaaa agcgaggaaa gccttgcgga
     1321 tactggattt taggcacaaa aaaagacgtc cgttgacgtc tattgatgtt ccgatggtgc
     1381 cgaaggccgg actcgaaccg gcacgtattt ctacggttga ttttgaatca actgcgtcta
     1441 ccgatttcgc cacttcggca ctgaagaggt atgcggaaaa cgttgtggat tatacctgtc
     1501 gcacgccacc atgcaagcgc cagcttgcac ccacctcgct aagtgctgaa attttcagca
     1561 ttacactcag cgatatgtaa cctttgtcac actccaggcc ccccgccctg ccatgctcta
     1621 cacttcccaa acaacaccag agaaggaccc aaaaatgtcg atgataaaaa gctatgccgc
     1681 aaaagaagcg ggcggcgaac tggaagttta tgagtacgat cccggtgagc tgaagccaca
     1741 agatgttgaa gtgcaggtgg attactgcgg gatttgtcat tccgatctgt cgatgatcga
     1801 taacgaatgg ggattttcac aatatccgct ggttgccggg catgaggtga ttgggcgcgt
     1861 ggtggcactc gggagcgccg cgcaggataa aggtttgcag gtcggtcagc gtgtcgggat
     1921 tggctggacg gcgcgtagct gtggtcactg cgacgcctgt attagcggta atcagatcaa
     1981 ctgcgagcaa ggtgcggtgc cgacaattat gaatcgcggt ggctttgccg agaagttgcg
     2041 tgcggactgg caatgggtga ttccactgcc agaaaatatt gatatcgagt ccgccgggcc
     2101 gctgttgtgc ggcggtatca cggtctttaa accactgttg atgcaccata tcactgctac
     2161 cagccgcgtt ggggtaattg gtattggcgg gctggggcat atcgctataa aacttctgca
     2221 cgcaatggga tgcgaggtga cggcctttag ttctaatccg gcgaaagagc aggaagtact
     2281 ggcgatgggt gccgataaag tggtgaatag ccgcgatccg caggcactga aagccctgtc
     2341 ggggcagttt gatctcatta tcaatactgt gaacgtcagc ctcgactggc agccttattt
     2401 tgaggcgctg acctatggcg gtaatttcca tacggtcggt gcggttctca cgccgctgtc
     2461 tgttccggcc tttacgttaa ttgcgggcga ccgcagcatc tctggttctg ctaccggcac
     2521 gccttatgag ctgcgaaagc tgatgcgctt tgccgcccgc agcaaggttg cgccgacaac
     2581 cgaactgttc ccgatgtcga aaattaacga cgccattcag catgtacgcg atggtaaagc
     2641 gcgctaccga gtagtcctga aagccgactt ctgatccctt tgccgggtgc tgtcgcccgg
     2701 cacttaaatc tctttggaag gcgtctcgca atctgctgaa tttagcggtg aagagtgcac
     2761 agccgttgcc tatactccag gcaggaaact ggaggaaatc tcatgagcga acccctgtta
     2821 attgcccgca cgccggacac agaactgttt ttactgccgg gaatggctaa ccgtcacggg
     2881 ctgattactg gcgcaacggg gacgggtaaa accgttacgc tgcaaaaact ggcagagtca
     2941 ttgtcggaaa tcggcgtgcc ggtgtttatg gctgatgtga aaggcgatct gaccggtatc
     3001 gcgcaggcag gaacggcgtc ggaaaaactg ctcacaaggc ttaaaaatat cggcgtcaat
     3061 gactggcaac cgcatgccaa tccggtggtg gtgtgggata tctttggcga gaaaggccac
     3121 acggtgcggg cgacggtttc ggatctgggg ccgctgttgc tggcgcgact gttgaatctc
     3181 aacgatgtgc aatctggcgt gctgaatatt atcttccgca ttgcggacga tcagggattg
     3241 ttgctgctcg actttaaaga tttgcgggcg attacccagt acatcggcga taacgccaaa
     3301 tccttccaga atcagtacgg aaatatcagt agcgcatcgg ttggtgccat ccagcgcgga
     3361 ttactgtcgc tggaacagca aggcgcagca cacttctttg gtgagccgat gctggatatc
     3421 aaagactgga tgcgcaccga tgccaacggt aaaggcgtta tcaatatcct cagcgccgag
     3481 aaactttatc agatgccgaa actgtacgcc gccagcctgc tgtggatgct ttcagagttg
     3541 tatgaacaat tgccggaagc gggcgatctg gagaaaccaa aactggtgtt tttcttcgac
     3601 gaggcacatc tgctgtttaa cgatgcaccg caggtactgc tggataagat tgagcaggtg
     3661 ataaggctta ttcgctcaaa aggcgtgggc gtctggttcg tttcgcaaaa cccgtctgat
     3721 attccggata atgtgctcgg ccagctcggt aatcgtgttc agcacgcttt gcgggctttt
     3781 acgccaaaag atcagaaagc agtgaagaca gcggcgcaaa ccatgcgggc caatccggca
     3841 tttgataccg aaaaggcaat ccaggaactg gggaccggcg aggcgttaat ctcgtttctc
     3901 gatgcaaaag gaagtccttc tgtggtggaa cgggcgatgg tgatcgcacc ttgttcgcga
     3961 atggggccgg tgacggaaga tgagcgtaat ggcctgatta atcactcccc ggtgtatggc
     4021 aaatatgaag atgaggtgga ccgcgagtcc gcctatgaga tgctgcaaaa aggctttcag
     4081 gccagtaccg agcagcaaaa taatcccccc gtgaaaggga aagaggtggc ggtggatgac
     4141 ggtattcttg gtggattgaa ggatattttg tttggcacta ccggaccacg cggcgggaag
     4201 aaagatggtg tggtgcaaac aatggcgaaa agcgccgctc gccaggtgac gaatcagatt
     4261 gtacgcggga tgttggggag tttgctgggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcatcttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agaatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcactgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtctggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggcg gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat ggcgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcggtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacccc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatc gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaa
//
LOCUS       DAJTYG010000106        11656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTYG010000106 DAJTYG010000000
VERSION     DAJTYG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.106"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            139..1824
                     /gene="btsS"
                     /locus_tag="OO568_003259"
     CDS             139..1824
                     /gene="btsS"
                     /locus_tag="OO568_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6642502.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            1821..2540
                     /gene="yehT"
                     /locus_tag="OO568_003260"
     CDS             1821..2540
                     /gene="yehT"
                     /locus_tag="OO568_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6642503.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            2587..3057
                     /locus_tag="OO568_003261"
     CDS             2587..3057
                     /locus_tag="OO568_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6642504.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(3098..3559)
                     /locus_tag="OO568_003262"
     CDS             complement(3098..3559)
                     /locus_tag="OO568_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR6642505.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(3684..5687)
                     /locus_tag="OO568_003263"
     CDS             complement(3684..5687)
                     /locus_tag="OO568_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCR6642506.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQELVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLNAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(5684..6820)
                     /locus_tag="OO568_003264"
     CDS             complement(5684..6820)
                     /locus_tag="OO568_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6642507.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(6813..7871)
                     /locus_tag="OO568_003265"
                     /pseudo
     CDS             complement(6813..7871)
                     /locus_tag="OO568_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136808.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8438..9154)
                     /locus_tag="OO568_003266"
     CDS             complement(8438..9154)
                     /locus_tag="OO568_003266"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642508.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPLQ"
     gene            complement(9165..10253)
                     /locus_tag="OO568_003267"
                     /pseudo
     CDS             complement(9165..10253)
                     /locus_tag="OO568_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
     gene            complement(10795..11202)
                     /locus_tag="OO568_003268"
     CDS             complement(10795..11202)
                     /locus_tag="OO568_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642509.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            complement(11263..>11656)
                     /locus_tag="OO568_003269"
     CDS             complement(11263..>11656)
                     /locus_tag="OO568_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HCR6642510.1"
                     /translation="RSEFAQLFADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKS
                     ATVGQLMGMRYKGWESGYEDAFVYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVY
                     RDNKSVTFAELDVFDLSEALSAPDVIFH"
BASE COUNT         2816 a         2958 c         3035 g         2847 t
ORIGIN      
        1 ctatagggtg attttatttt tgccaggcga ttttgagtga tcgtactcac gaattctcat
       61 ttttctgcaa gagttcaaag aaagttaaac gcaggcaatg tatgttacgc gttttaaagg
      121 gaagtgtggt ttgcgggtat gtacgatttt aatctggtgt tgctgctgct tcagcagatg
      181 tgcgtttttt tagtcattgc gtggttaatg agtaaaacgc cattattcat accgttaatg
      241 caggtcacgg ttcgtctgcc gcataaattt ctctgctaca tcgtcttttc catcttctgc
      301 atcatgggca cctggtttgg gttgcacatt gacgattcta ttgccaatac ccgtgcgata
      361 ggcgcggtaa tgggcggctt actcggcggt ccggtcgtcg gtgggctggt tggtctgacc
      421 ggcggcttac atcgatattc gatggggggc atgaccgcgc taagttgcat gatctcaacc
      481 atcgttgaag gattgctcgg cggcctggta cacagcatcc tgatccgtcg cgggcgcact
      541 gataaagtct ttaaccccat taccgccggt gccgtcacgt tcgtcgctga aatggtgcaa
      601 atgctgatca tccttgcgat cgcccgacct tatgaagatg cggtgcgtct ggtgagtaat
      661 attgctgcgc caatgatggt caccaatacc gtcggcgcgg cgctgtttat gcgtatattg
      721 ctcgataaac gcgcgatgtt tgaaaaatac acttcggctt tttctgccac tgcgctgaaa
      781 gtggcagcct cgacggaagg cattttgcga caggggttta acgaagtgaa cagcatgaaa
      841 gtggctcagg tgctgtatca ggagctggat attggtgcag tcgcgattac cgatcgagag
      901 aaattgctgg cctttaccgg aattggtgac gaccaccatt tacccggcaa accgatttct
      961 tcgacttaca ccttaaaagc gattgaaacc ggtgaagtgg tctacgctga tggcaacgaa
     1021 gtaccttatc gttgctcttt gcatccgcaa tgcaaactgg ggtcgactct ggtaattccg
     1081 ttgcgtggcg aaaatcagcg cgtgatgggc accatcaaat tgtatgaagc caaaaaccgt
     1141 ttattcagtt caataaaccg cacgctgggc gaggggattg cgcaactgct ttcggcgcag
     1201 attcttgccg ggcaatatga gcggcaaaaa gcgatgctca cccagtcaga aatcaaactg
     1261 cttcacgccc aggtgaatcc tcattttttg tttaatgcgc ttaacaccat taaagcggtg
     1321 atccgccgcg acagcgaaca ggccagccag ctggtgcagt atctttccac ttttttccgc
     1381 aaaaacttaa agcggccttc ggagtttgtt actctcgccg acgaaattga acatgtgaac
     1441 gcttatctgc aaattgaaaa ggcgcgcttc cagtcgcggt tgcaggtcaa cattgctatt
     1501 ccgcaagaat tatcccagca gcaattgccc gcgtttaccc tgcaaccgat agtggaaaac
     1561 gccattaaac atgggacatc acaactgctg gatacagggc gagtggcaat cagcgcccga
     1621 cgtgaggggc aacatttgat gctggagatc gaagacaatg ccggtttgta tcaaccggta
     1681 accaatgcca gtgggctggg gatgaatctg gtggataagc gtttacgtga acggtttggc
     1741 gatgactatg gaataagcgt cgcctgtgag cctgatagtt acacccgaat aacgttacga
     1801 ctaccatgga gggacgaggc atgattaaag tcttaattgt cgatgatgaa ccgttagcac
     1861 gggagaacct gcgtatattt ttgcaggagc agagcgatat tgaaatcgtt ggagagtgtt
     1921 caaacgccgt ggaagggatc ggcgcggtgc ataaactgcg cccggatgtg ctgtttctcg
     1981 atatccagat gccgcgcatc agtggtctgg aaatggtggg gatgcttgac ccggaacatc
     2041 gcccgtatat tgtttttctc actgcgtttg acgaatacgc tattaaagcc tttgaagaac
     2101 atgcctttga ttatctgctg aagccaattg atgaagcgcg actggagaaa acgctggcgc
     2161 gattgcgtca ggagcgcagc aagcaggatg tttcgctgtt accggaaaat caacaggcgc
     2221 tgaaatttat cccttgtacg gggcatagtc ggatttattt gctgcaaatg aaagatgtgg
     2281 catttgtcag cagtcggatg agcggtgtct acgttaccag ccacgaaggg aaagagggct
     2341 ttaccgaatt gacattacgt accctggaaa gtcgtacacc actactgcgc tgccatcgtc
     2401 agtatctggt taacctcgcg catttacagg agattcgtct ggaagataac ggccaggccg
     2461 agttgatttt gcgtaatggc ttaaccgtgc cggtcagccg ccgttatctg aaaagcttaa
     2521 aagaggcgat tggcctgtaa aagactgcta aaatggcttt ttgcctcatc aacacctgaa
     2581 ggcctcatga taagtaacga tattctgcgc agcgtgcgct acattttgaa agccaataat
     2641 aatgacctgg tgcgtattct ggcgctggat aatgtcgaag ccaccgcgga acagatcgcc
     2701 gtctggctac gtaaagaaga cgaagagggt tttcagcgtt gtccggacat tgttttgtcg
     2761 tcattcctca atggcctgat ttatgaaaaa cgcggcaagg atgagtctgc tccggcactg
     2821 gagccggaac gtcgcattaa taacaacatc gtgctgaaaa aattacgcat cgcgttttcg
     2881 ctgaaaaccg atgacattct ggctatcctc accgaacagc agttccgcgt ttcgatgccg
     2941 gaaattacag cgatgatgcg cgcaccggat cataaaaact tccgcgaatg cggcgatcaa
     3001 tttttacgtt attttctgcg tggactggca gcgcgccagc atgtgaagaa aagctaagac
     3061 gggtatggcg gccatgcgaa acatggccgc cgacagatta tttcacttct ttaaaaccag
     3121 cggctttcat caccagttcc atttgcgcca tagtgatacc ttttttggca tcttcagcag
     3181 aaacgttgat tcctgaaata ccctgcaggg ctttaaaatc cactttttcc atatcgatag
     3241 tcacgttttc ctgcgcgtag gtatcggtat aggttgattt ttcttcaaca cccgcgatgt
     3301 ttttgtattt ggcgcttaac ggctcaagtg tcttggcagc atcttctttg gtggttgcac
     3361 caatggaggc aaattgaatt ttggtttcag aagattgctt aagcaccttg tcacctttgt
     3421 agacataggt aatggcaatt tcagtgccgt tcagattggc gctgaatttc ttcgattctt
     3481 ctttgtcacc gcagccagca agagagaaaa ccagaacaga tgcaacaacg agggaaaaca
     3541 gcttattgaa agccttcatg taaaactcca ttttatttaa tcaagaaact ggtgactctc
     3601 accaggggct atataggata tgcctaatac cgtggcgtga gcagtccgga actggagtag
     3661 aactcttagt aaaaagcact atttcatcct tgttgctgaa gcatggggaa taattgttcg
     3721 caaagcaaaa caccgttatt cattgcttct acccgtgcct cgctttctgt attacgaaat
     3781 tgtgccaaca catgtgccag ccgataaaaa cccaccgcgg tgaggtcatt cgccagcaac
     3841 tctgcctgac taatagcgct ctgttcctga tagcgccagc cgttatggag cagttgaata
     3901 agtaacgcct ggcagcgcat cagcaactga tgagcagtag acggcacagg cagaacgctg
     3961 gcagaaggta gtgataccac cacaggcgca gtttctgcgt ccagcgccca ggcacgagtc
     4021 tttgtcatca ttacctgtgg ttccagtgtc aattgcccat caacaaaact gacaaagcca
     4081 gaaaccagac acacggggtc gtctgtttgt tgcaaaagcg ccgccatgcg ttcaatggca
     4141 taaggcgcgc tggctgaggc tggcagtgat aacgtcagca gattatcttc accttcgccg
     4201 ctgattacct gcgcatccag cgtctggcgg ctgctatccc aaccgagcga aatacactca
     4261 gcgaccggca gaataaataa gttatcgacc tgattaagag gccgtatgca ggcgggggga
     4321 cgctggcgta aatattcccg caaagccaca atgcccggct ggcgtaacgg cgcgttcaac
     4381 atttgccagg catcaggcga cagcggtaca acgctgctta agcggttgcg ggtagctaac
     4441 agcagctcgc catcggcact gcgttttgct gcttgtgaaa caatttgccc acccgccagt
     4501 gcgccagcct gaaaactaaa cagccgacgc gtagctgccg gtgagtcttc ctgttcactt
     4561 cgcggccaac tgcgcgaaag gtgcaaaata ctgccggtgt cgggatcggt aaaccagatg
     4621 cgtaaaccat aatgctcaat atcctgccag caacgcatac ctaaagacac cagccgcaga
     4681 tgatcaagct ttgcttctcc ggcaatgcca gagccaacga ccgtgcgcca cggcacagga
     4741 ggaacttcac caacactgtc gctccgggcc atctcttgtg cgcaatttaa tcgactgttt
     4801 aatgccgcaa gctgacgtaa gcattctccg gcatgatagt ggctggcgcg ggcgtggaag
     4861 gcatcaacgc tggcgcgcag ttgccgtagc gattcactca cccatcgcca gctgcagcgt
     4921 tccgctgctt gctgcgcgcg gctgaaagcg gcctcgtagt gaataagcgg ctggctgatg
     4981 ccgctcagcc ataatgcctg gcttagttgt tgaacatatt gacgacacgt tttgccttct
     5041 tcgctggcaa acggatcgtc agatgatgtg acgtgttcgc tgcgcatctg ccagattaaa
     5101 tgagtcaatt ccgcttgctg cgcttttgcc tggacaaagg cctgtactgc cagtacgaca
     5161 tgttcgcaaa gtgtgccttc aatacaatca caacgggcga aacgaatact gctgcgggaa
     5221 taaaaacgca catcgctcat cggtaagcgg gcagagggaa tttcacccgg cgcacagaac
     5281 aactcaatgg tgatgctttt agcgaccagc tcctgtgcgc gtttgcgggt ggcatcggga
     5341 agggtagcca gttcttccag ccagattgcc ggatcccatt cctcttcttt tcccgtaggc
     5401 tgggcggtag cacaaagtcg ttgataactt aacaccagca tcacgcgatg acggcacatg
     5461 ccgctggccc cacaggtgca ctgagcctct ttcagtgcct ggccgttcgc cagctgggta
     5521 cggacaccgt cactgaaggt ggcgattaaa gcgctgttct catggctgat ttccgggacg
     5581 ttgccatttt ccagttcctt aaggctgcgc ttaacaaaac cggcattgct taacgccgtc
     5641 agtgcctgcg gtgtcagttc taataattcc ggacgtagtg aattcatgac tgaagattct
     5701 ccgcaagcca tgatgccagc tcgcccggtg tcatggcggc tatttgtgcg ccgacattaa
     5761 ccagcgcctg ggccgtatcg cggtcatagc aaggtgttgc tgtgctatcg agcgctgcca
     5821 gtcccagcac tttgatgccg ctctggacac actttttcac ttggtgcgtc agcaatgatg
     5881 atgaaccccc ttcataaaaa tcgctcacga ggataatgac gctttttgca ggttgttcaa
     5941 taagttgccg accatactcc atggcactgg cgatattggt tccgccgcct aattgcactt
     6001 tcattaataa ctctaccgga tcggcaacgt ctgccgtgag atcaacgacg cttgtgtcaa
     6061 acgccaccag atgggtacga atgccgggta actgccataa acaggccgcc atcaccgcag
     6121 agtggatcac cgaatcgacc atcgatccgc tttgatcaac cagtaagacc agttgccatt
     6181 gctcgctttg gcgtttaatg cggctgttaa agcgggggaa ttcgatatac aacttgccgt
     6241 gttgcgggtg ccagtgttgc aggttggcgc gcagagtact tttgaaatca aagtttcgcg
     6301 ccagtggaat aaatgagcgg cgacgacgat cgcggacacc agaaaaagcc tgacgaactt
     6361 cctttgccag tcgcgccata atttcttcca caacctggcg cacaatcctg cgggcggcag
     6421 ccagtacttc ggggttcatc agatgtttgg tgtgcaaaac ggcgcgtagc aggctttcgg
     6481 aaggctgcat acgttccagc acgtcgagat ttgtcaccac atcttcaatg ccgtagcgca
     6541 gtacggcatc gctttccagc cgctcaatca cctgttgcgg aaacagcgtg tgaatactgt
     6601 tgatccactc aggagtggtg agatttgagc cacctaatcc actagagcgt tcaccacgct
     6661 ggagccgttc aggatcgcgc ccatacagcc actccagcgc gtgatctatc tgccgggcgt
     6721 tgtcatccag cccacaaagc gtcgtttctg ccgcttcgcc aagaattaat cgccagcgtt
     6781 gtagctcacg ggtggtcaga agatcgttca gttcagacat ggaaaactcc ccagtgttgc
     6841 agcgatgcca gtgcctgctg ttcgagttgt tgatgatgtg caatggcttg cggtggacaa
     6901 tgcaacggca tttgcagtgc cgaaacggga agttgcgcca gttgataatg ctcaagcacc
     6961 tgatgcgcca gcgtcccgcg ttctcgtggc ggtagccagg ccattgccgc gcgtaaatcg
     7021 ggcagggcat tggtaaaatc ggcatcactt agttgattta aatgactgct gaaaccggcg
     7081 ataaatgccg gctgacaggc cagttgatga cgggctaatg ccagcaaacc gtgcagcgcc
     7141 tcgccggatt gtgccgggga taactgcgcc agcatcgtca gcgccgcttc ggcactagcg
     7201 ttgggatgtt ccagacgcat cagcgcacca agagtcgcgc cgcgatccag tgccaggaca
     7261 tgaatcgcct gactgtgtcg ctccagcaag gctaccgccg cagaaagcga aacgccgggg
     7321 agttgcacac cgctatgtag atcgcgcagg atatgacaca acgcttgcca gctatgcaag
     7381 tgagcgtgaa actccttttc atccggtctg ccgttggatt cacacagcca cagcgtgcga
     7441 tcgatagccg cgcataacgt cgtttgtaat atctgcgcgc cttgcatacc gctaatttca
     7501 tccagccgcc ataaggcata tagcacttcc agcgcctggc ccatttcggc aaattgattt
     7561 tcctgggcga ttaataatgt gagttgctcc agtaattgtt gactgaagga cgccagaccc
     7621 gctgacgccg cctggctaag acatgtggta atgctgccga ttccacccgc gtccagcata
     7681 tcggcttcta atttatggcg tgcggcttcc tagagtgtgg caccaaagca ggcggcctca
     7741 atcaatgccg catgctggct aagcgggcgg gttaatttcc agcgttcttc accgttgcct
     7801 gcaagtgtca gtgtgcttcc ctgctggcgt acaatccctg ggatctccag tattgccagc
     7861 cgatgtaaca cctgactttg cgctagtcca ttggggttaa agcgatttaa ggtaagctcc
     7921 gccggaagag agattgcggt acgttccagt tcgcaagtga catcttttgg cagaggcggt
     7981 tgtggcgtag aaggggcaag tttaccgaat ccgtcacccg ctaatgtgtc tatcaacgtt
     8041 agcagaatcg gatcggtatc gggatgaata acgccgcgat agctccacgg caacggcgca
     8101 ttcagggctt ctttaatcag cgatcctgct atggcatcca gccagtcagt gcgtaacggt
     8161 agtgtatgac cgcgcaactg tgccagtgcc atcgcatgca gatgagcggc agccatatcc
     8221 gcggtcgaag caggcaactt gtgctggcgc aaacgggtga gaaccgtttt tagcagttgt
     8281 tctcccgcct gctgtaagcc ccactgccag caccagtttt gccagaccgg ggcaggcatt
     8341 cctgaaaggt atcctgccag cacatcaagg cgcttttcac tgtagggggg tgagataaca
     8401 acctgtaacg gcatctgcca gcacatcaag gcgcttttca ctgtaggggg gtgagataac
     8461 aacctgtaac ggcatctgcc agcgacggca attctggcgt gttaattacc tgcggacatt
     8521 cgcgccacat ttttgccagt gccggagcgt gccagccacc gcagacaact aacacgtcgc
     8581 cattattttg ctgcatcgcc catccaatcc agcgggccat aaaggcttcg cgctgacgat
     8641 tgagagcatc gccgggggag tcgccccgca gttgggcaaa atagtgcgcc agtgcagagg
     8701 gtaatgcagt ttgctggctt tcatcttcga acaagtgatc ccacagggtg tcgctgttat
     8761 ccatgcgggt ggcacgcagc agcaaggtct ggctttcctc ttgcgtatca ggcgaatcgt
     8821 cctcttcttc actctgcgcc cagcaaggca aatcgatgaa gtaagtttgt gcctgaatac
     8881 gacgatcggc ttgtagcgcc tgccactccg gcgaaaattc agcaaatggc gtccaggcac
     8941 cacgcccggg ggctgcaccg tcctgatact ggcaataact gtaaatagct accggaagct
     9001 ggtgggctaa aaacagttcg tctacccggt cattaaaatc agccgggcct tcaatcaaca
     9061 cgtatcgtgg ccgctggctt tcgattaacg atttcaccag ccgggcgcag gccggactat
     9121 gatgccggat gccgacaatt aacggctcgc tcatgcatct ttcctcacgg caggcggtgg
     9181 cgggcttgat aataagcctg ccagtgagct tctttatgtg tggcgacacg ctgttcgaag
     9241 taacggcgca gacgagcgcg atcttcttcg ttatctttga cgatggttcc ggcaatgcag
     9301 tccaccaggt cggctggctc acctgcgcga ttcgctaaaa accaggcgcg gacgcctacg
     9361 gcgtgggcca cgttcacggc ttcagcggtg gacataatcg ctgttagtgt atccattgac
     9421 gttttctttt cgccgttggc acgcagatcg cggaaggtgc ggaccagtaa ctccagtacc
     9481 gcgtcaggca ctttatgcgg aataccgcta tgggccagca aacgcgccga agcactggcg
     9541 acaagctcca gctcctgggc gaaatccata atcggaaaga cagtttcaaa atcgaagcga
     9601 cgctttagcg cagcactcat ttcattgacg ccgcgatcgc gggtattggc agtggcaata
     9661 atattaaagc cttctcgcgc atagagctga ctggcttcac cagtgagttc cggcaccgtc
     9721 atcaccctgt cagaaagcat acctaacaga cagtcttgca cttccagcgg tgtacgcgtt
     9781 atctcttcaa aacggacgat tttgccgtcg cgcattccct ggtaaagcgg ggcagggacc
     9841 agcgcttccg ttgatggacc atgattgata agcagggcgt agttccagcc gtacttgatt
     9901 tgatcttcgg tagtagatgc gccgccctgg atggttaacc cggcatcgcc gctaatagag
     9961 gttgccagta attcagacag gagagatttt gcggtgctag gttcccccac caacatcaaa
    10021 ccgcgtccgg tggcaagagt gactaacata cgttcgacag cggagggact aatcaccact
    10081 ttacggctaa tattttgcgc gctatcgcca aggataaaag cacgggccgc aggcaaactt
    10141 aactaccaac cgggcgggca aggtgcgtta tcattttgtt ttaattttgc cagttcatcg
    10201 gcgtataaca cagcagcagg cggacgctgc agatgattat tctgcggtga catatattca
    10261 tccctgaaat gtattaatga aataaacgat attttacgcg caataaatgt ttggcgcatt
    10321 agttgtttga gcgaaaaata tctggaaagc agatcacagt aaataaaaaa acctgctggt
    10381 gaggcaggtt cttttattta ctattttcag gcctaacacc actgcctgtg caacatcgtg
    10441 ttccaacgaa cgaattataa ccacctctgg acagtgttgc aattaagata atgagattta
    10501 taatcctgac tgttgggaag ttgcgccgga tcgaacccat ttcaagtcct ggctaccgga
    10561 cgggtggtgc cgcaggtagt gctctgatcc ggagtgcaac atggcgactc aacaaacgta
    10621 aaccagtatc gacaggcagt gtaagggtaa ggtgctggtg ttgtgctcct taataaggag
    10681 agcggatgga cacgctgaca caaaagttaa ccgtgctcat tgctgtactg gagctattag
    10741 tggctctgtt acggttgatt gatttgttga agtaacgggc aaattgaaac acatctaagg
    10801 gggaataccg ttcccccctt taataaagct atactcgtca tacttcaagt tgcatgtgct
    10861 gcgacttgaa ttatctagag tatataattc tatttgttgg ttgaatatat tctgtagttt
    10921 acaattaaaa attactatta caggttcagg tgattcgcct cgtaattttt gctcatcaat
    10981 aaactcatct cttatataaa tcaacgcatc atttattagc gggtcaacat cctctcgatc
    11041 acgttgatga aatgttggac gtagccggaa ccgttccagt cggtagacgg aacctgtaaa
    11101 taattcagag catttatgga tctcaagtct gaaggaaaac ggatcttcag gtacagggac
    11161 ctcataatcg taaatagcaa ccagctcttt ttgcacgatc atcgctttcc ccgctggtaa
    11221 gtcgtgtaga taacgggcag tcgcatcact gcccgctttg ttttaatgga aaatgacgtc
    11281 aggtgcgctt aacgcctcac tcaaatcaaa cacatcaagt tcggcaaaag tgacggattt
    11341 attgtcacgg tacactcgaa gagaacggaa gctcattagc gctttgctat caacattgta
    11401 gtggttaaaa ccgggtgaaa acttaaggac cagccggtac tcacccaggt catagacaaa
    11461 tgcgtcctca taacctgact cccagccttt gtatcgcatt cccataagtt gcccaacggt
    11521 agcggattta ccttcccagc gagtcaggct gttactggtt gactcgtcag gtgtgagcag
    11581 caccgtgcgg cgcgacaact ggcgaaaagg cggcataatt tcgtaatcgg caaaaagctg
    11641 tgcaaattca ctgcgc
//
LOCUS       DAJTYG010000107        11545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTYG010000107 DAJTYG010000000
VERSION     DAJTYG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.107"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            9..461
                     /locus_tag="OO568_003270"
     CDS             9..461
                     /locus_tag="OO568_003270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6642511.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            707..1751
                     /locus_tag="OO568_003271"
                     /pseudo
     CDS             707..1751
                     /locus_tag="OO568_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1813..2427
                     /locus_tag="OO568_003272"
                     /pseudo
     CDS             1813..2427
                     /locus_tag="OO568_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="OO568_003273"
     CDS             complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="OO568_003273"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6642512.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4203..4661
                     /gene="gpt"
                     /locus_tag="OO568_003274"
     CDS             4203..4661
                     /gene="gpt"
                     /locus_tag="OO568_003274"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6642513.1"
                     /translation="MSEKYIVTWDRLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4753..5997
                     /gene="frsA"
                     /locus_tag="OO568_003275"
     CDS             4753..5997
                     /gene="frsA"
                     /locus_tag="OO568_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6642514.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6055..6456
                     /gene="crl"
                     /locus_tag="OO568_003276"
     CDS             6055..6456
                     /gene="crl"
                     /locus_tag="OO568_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6642515.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="OO568_003277"
     CDS             complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="OO568_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6642516.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7838..8941
                     /gene="proB"
                     /locus_tag="OO568_003278"
     CDS             7838..8941
                     /gene="proB"
                     /locus_tag="OO568_003278"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6642517.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8953..10206
                     /gene="proA"
                     /locus_tag="OO568_003279"
     CDS             8953..10206
                     /gene="proA"
                     /locus_tag="OO568_003279"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6642518.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            10321..10396
                     /locus_tag="OO568_003280"
     tRNA            10321..10396
                     /locus_tag="OO568_003280"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10354..10356,aa:Thr,seq:cgt)
     gene            complement(10411..11439)
                     /locus_tag="OO568_003281"
                     /pseudo
     CDS             complement(10411..11439)
                     /locus_tag="OO568_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2769 a         2859 c         2973 g         2944 t
ORIGIN      
        1 tttatattat gaataacata caaataagag attatcagcc tggcgatttt cagcaactat
       61 gcgctatttt cattagagcg gttacgatga ccgtcagtca gcattattca ccacaacaaa
      121 ttgccgcctg ggcgcagatt gacgaatctc gctggaagga gaaactcgcg aaatcacaag
      181 tgcgggttgc agtcattaat gcaaaactgg tcggctttat tacctgcgtt gaacactata
      241 tcgatatgtt atttgttgac cctgaataca cccgccgtgg ggttgccagc gctttgttaa
      301 aaccttttat taagtctgaa tccgaactta cggtggacgc aagcataacc gcaaaaccct
      361 tttttgaacg ttatggtttt cagacagtta agcagcagcg cgttgaatgc cggggagcgt
      421 ggtttattaa tttttatatg cgttataaac cgcaacatta aatccagctt gcaatgaaaa
      481 taacgcccgc ctagtatgtg cgcgttttct tattccacaa actgcaagga ggtaaatcat
      541 gacaaatcct ttatcaatga ctctttgcag acctttccag gattaattct ttttttcttg
      601 ccctggattc gtctgccatt tcctgatttt ttaattgtta ctctcaataa ttcaagtcgc
      661 agcacatgca acttgaagta tgacgagtct atttacggaa tggtatatgg gcaattatat
      721 tcgtccctta tccgatgcgg tatttaccat cgcatctgat gacctgtgga tcgagagttt
      781 agcgatccaa caattacaca ccacggcaaa tttacccaac atgcagcgcg tagtggggat
      841 gccagattta caccccggac gcggctaccc gattggcgca gcgttcttct ccgcaggccg
      901 tttttaccca gcactggtcg gcaatgatat cggctgtggc atggcgctat ggcaaacaga
      961 tattctcgct cgcaaataca acgccgataa gtttgaaaag cgattatcag ctttggatga
     1021 cgttgctgaa gaaagctggc tggaggaaaa cctgccgtca gcatttgcac agcatccgtg
     1081 gcgcagctcg cttggctcta tcggtggtgg caatcatttc gctgaactgc aacaagttga
     1141 tcaaatcatc aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc atctgcaact
     1201 gctggtccat agcggctcga gggagctagg gcagtctatt ttacagcggc atattgcctc
     1261 gttttcgcat catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg cgcgttttaa
     1321 tcgccacctg atcgctttgc gcataatgca acaggttaag gccaccggaa gtccggttct
     1381 ggatgtggcg cataactttg ttagcgcgtg tcgaatcggt gatcaacaag gggtcttaca
     1441 tcgtaaaggt gccacgcctg atgactgtgg tctggtggtt attcccggtt cacgcggtga
     1501 ttactccagg ctggttcagg cccgtcagga gtgaggaaac attgcattcg ctggcgcatg
     1561 gggctgggcg taaatggggg cgcaccgagt gtaaagggcg tctggcagcg aaatacacag
     1621 cgacgcaact ctcgcgtact gaacttggca gtcgggtaat ttgtcgcgat aaacaactca
     1681 tcttcgaaga agcgccacaa gcctataaat cggctgaaag tgtggtgcaa tgtctgggca
     1741 ggccggatta attattcctg tcgcgcggct gcgtccggtg ctaacgctca aaaacagtgg
     1801 agggaaaaaa ggatgatctt gctataactc tcctcagcgc aggggccgga agaatgttgt
     1861 ctcgcagtga aaaaggcgct ggacaggcta attaaagaag ctgcccgaca agacgtcgcg
     1921 gtaacggtgc tggaaacaga aacaggccgc tactctgaca cgctgcgttc ggcgctgatt
     1981 tctctggatg gcgacaacgc atgggcgtta agcgaaagct ggtgtggcac tattcagtag
     2041 atttgcccga gcccgtatcg cccacatcac gggcgtaaaa actggtttct gggcattggg
     2101 cgttttaccg ctgatgagca ggagcaatcc gatgcgatcc ggtatgagac gctgcgttcg
     2161 tcggggccgg gcggtcaaca tgtcaataaa accgactcgg cggtacgcgc cacgcatttg
     2221 gcatccggta ttagcgtgaa ggttcagtca gagcgcagcc agcatgctaa caaacggctg
     2281 gcgcgattgc tgattgcctg gaagctggat cagcagcaat aggaaaatag cgcgtagctg
     2341 aaatcacagc ggcgaatgtt ccatcaccag attgaacgtg gcaacccgcg acgaacgttt
     2401 acagggatgg cttttatcga aggataatga aggatgaaac tgccggaagg cgattaaacg
     2461 ccatccggca gcaaatcaaa taattacttc gccggaattt ctttcagcag ttcagtcagc
     2521 agtgtccagt aatgacctac gctttcgatg tgaacttgct catccggaga gtgtggaccg
     2581 gtgatagttg gcccgataga aaccatgtcc atttccggat acggtttttt gaacagacca
     2641 cattccaggc ccgcgtggat aatctggatg ttcggcgtct tgttgaacag gcgctgatag
     2701 gtttcacgta ccagatgcat caccggagaa ttagcgtccg gctgccagcc aggatatgcg
     2761 cctttcgctt cggttttcgc gccagccagt ttacccagcg aatccagcat gctcaccacg
     2821 tagtctttac cgctgtcgat cagtgaacgg atcaggcagt gaatttctac gttattgtca
     2881 gtcatggtca ccacaccgac gttcagggag gtttcgacca caccttttgc cacgtcggaa
     2941 ttgcggatca caccgttcgg ggtggcgttc agcagacgaa taaaggtatc gcgagatttc
     3001 gcaatcaggg cagctttatc gttcgctaca gagtccagca acaaggccag atttttctct
     3061 ttctctgcca gctcgttttt caggatctcc tgataggtat tcaccagaga tttcagtgcg
     3121 tcgactttat cagctgtgac agcaatggtc gcaaaggctt cacgcgggat ggcgttacgc
     3181 agtgtgccgc cgttgaaatc gataaggcgc aggtccagtt cttctgcatg acccgccagg
     3241 aagcgcacca gcagtttgtt ggcattaccc aggccaacgt ggatttcacc gccggagtga
     3301 ccgcctttca gaccttttaa ggttaacttg aaggtttcaa aaccagctgg aaccgcttca
     3361 cgatctaaat gcaggttgga ggtgaagtcg ataccccccg cacaacccat gtagatttca
     3421 ccttcttctt cggagtcggt gttaatcaga atatcagcct gcaaccagtt gctctgtaag
     3481 ccgaacgcac cgtccatacc ggcttcttcg gtcatggtca gcagcacttc cagcgggccg
     3541 tgaaccacgt tttcgtcagc cagaaccgcc agtgcagagg ccataccaat gccgttatcc
     3601 gcacccagcg tggtgccgcg cgctttaacc cattcgccat caatataagg ctggatagga
     3661 tctttcgtga agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc gaggtgggcc
     3721 tgtaagacga ccggtttacg attttccata cctgcggtag caggtttacg aatcaggata
     3781 ttacctacct gatcgcgttc gacatggaaa cctttctctt ttgcccaacc aacaatgtat
     3841 tcagcgagtt gctcttcatg ataggacggg tgaggaatag aacagatttt ggcaaaaata
     3901 tcccacagcg gctgtggaga taattgagac agttcagaca cgttaagtct ccttgtcgat
     3961 cacccgcaaa acagtattgc aggtcacagg gttagcagaa aatgttgtca acacaagaca
     4021 ggcttgcgag atatgtattg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     4081 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatatc tataatctcg
     4141 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     4201 acatgagcga aaaatacatc gtcacctggg acaggttgca gatccatgca cgtaaactcg
     4261 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     4321 tggtaccggg ggcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
     4381 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
     4441 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gctgttgcga
     4501 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
     4561 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggatcgaa cagccgtggg
     4621 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcat
     4681 tgccgggcgt tgttcttttt aacttcaggc aggttacaat agtttccagt aagtattctg
     4741 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
     4801 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gtgcacaacc gcctgtgcag
     4861 tcggctcttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
     4921 atctggcgcg gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
     4981 gatgctgaac gtaccgacga tgatttatac gataccgtga ttggctaccg tggtggcaac
     5041 tggatttatg agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg tacggaagaa
     5101 gatccgcaac tcagtggtcg acactggctg catgcggcta cgttgtacaa cattgccgcc
     5161 tatcctcatc tgaaaggaga tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc
     5221 tatgaagagg ccgcccagcg tctgccgggc acgatgcggc agatggagtt taccgtaccc
     5281 ggcggtgcgc ccatcaccgg ctttttgcat atgccgaaag gcgatggccc gttcccgaca
     5341 gtattaatgt gtggtggtct ggatgcgatg cagacggact attacagcct gtatgaacgt
     5401 tattttgcgc cgcgcggcat tgcgatgctg actattgata tgccgtcggt gggcttttct
     5461 tcaaaatgga agctcaccca ggactccagc ctgttgcatc agcacgtctt aaaggcgctg
     5521 cctaacgtac cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg tttcggcgct
     5581 aacgttgccg tgcgtttggc ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt
     5641 ggtccggtag ttcataccct gttgagtgat tttaagtgcc agcaacaggt gccggaaatg
     5701 tatcttgacg ttctggcgag tcgtttgggg atgcatgatg cttccgacga agcgttacgc
     5761 gtggagctga atcgctattc attaaaagtg caaggattgc tggggcgtcg ctgcccaacg
     5821 ccaatgttat caggctactg gaagaacgat ccgttcagcc cggaagagga ctcacgctta
     5881 atcacctcat catctgctga cggtaaatta ttagagatcc catttaaccc ggtgtatcgg
     5941 aattttgaca aaggtcttca ggaaatcacc ggctggatcg aaaaacgctt gtgttaaaaa
     6001 tttgctaaat tttgccaatt tggtaaaaca gttgcatcac aacaggagat agcaatgacg
     6061 ttaccgagtg gacacccgaa gagcagattg atcaaaaaat ttaccgcact aggcccgtat
     6121 attcgtgaag gtaagtgcga agataatcga ttctttttcg attgtctggc tgtatgcgtc
     6181 aacgtgaaac cggcaccgga agtgcgtgaa ttctggggct ggtggatgga gctggaagcg
     6241 caggaatccc gttttaccta cagctaccag tttggtctgt tcgataaagc aggcgactgg
     6301 aagagtgttc cggtaaaaga tactgaagtg gttgaacgac tggagcacac cctgcgtgag
     6361 tttcacgaga agctgcgtga actgctgacg acgctgaatc tgaagctgga accggcggat
     6421 gattttcgtg atgaaccggt gaagttaacg gcgtgagtga aatgtgccgg atgcatcaca
     6481 tccggcaata ttcattaaaa ctgatacgtc atcccaaccg cgacagtatc atcattatta
     6541 atattcagct tattatcgct atccagtttg ttgattttat aatcaacaaa cgctgacata
     6601 tttttgttga aataatacgt agcaccgacg tcgatataat tgaccagatc ttcatcaccg
     6661 atgccctcaa tatctttccc tttcgataag acataaccca gcgatggacg cagaccaaag
     6721 tcaaactggt attgagcgac cgcttcaaag ttctgtgtct tattggcaaa gccgccagtt
     6781 attggcgtca ttttgcgtgt ttcagaatag aaagttgcca gataaatatt attggcatcg
     6841 tatttcagac ctgttgccca tgcttctgca cgcttgcctg tgccacggct ttgcaggttc
     6901 tgctcgttgg tgcgatctga gttggtatag gccccactaa tggcgaaatc gctgccgcca
     6961 aagtcatatg tcaatgacgt gccgaagcca tcgccgtttt gctttttaac gtcgcggttt
     7021 tcgtttttcc cttgatattg cagggttaag ttcaggccat cgataacgcc gaagaagtcg
     7081 gtgttccgat acgtcgccag accgctggcg cgtttggtca taaagttgtc ggtctgcgcc
     7141 gaggaatcgc caccaaattc cgggaacata tcggtccagg cttccacgtc atacaacgcg
     7201 cccaggttac gaccataatc gaaagaaccc aaatctttat atttcaaccc ggcaaaagcg
     7261 agacgcgttt tttgctgtgc agtatcactc tccgctttat ttccggcaaa ctccgcttcc
     7321 caccgaccat aaccagtcag ttgatcgtta atttgagttt cacctttaaa accaaaacgg
     7381 atataactct ggtcgccatc tttactggcg ttatcactca tataatgcat ggctttaact
     7441 ttgccataga catccagttt attaccgtct ttattatata tttctgcagc ctgtacagat
     7501 gcagatgcca caatgcccat taccactaat gccagagtgc tctttttcat tttcattcct
     7561 gattttaatt aacgcgcgaa tattcagcgg gagagccccg ttgaaaacag gaaagttttt
     7621 aacctgagat tgttaaagat atattacaga ttaatgatat tctgaaaatg tggtaattta
     7681 ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc gcgcaacaaa
     7741 acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa aacgttcgtt
     7801 tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc agacgctggt
     7861 ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc gtgcccatat
     7921 cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga ttgttattgt
     7981 gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaat tgccagcgac
     8041 tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc aactgtggga
     8101 acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc gtgctgatat
     8161 ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc tcgatcacaa
     8221 tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta aggtcggcga
     8281 taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac tgttgctgct
     8341 gaccgatcaa aaaggtttgt ataccgctga tccgcgcagc aatccgcagg cagaactgat
     8401 taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca gcgtttcagg
     8461 cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt gccgtgcggg
     8521 tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg tgatggaagg
     8581 catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc gtaaacgctg
     8641 gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa ctgccgccat
     8701 tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg gcaatttctc
     8761 gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc acggtgtcag
     8821 tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag aaattgatgc
     8881 aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga ttacccgtta
     8941 aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc gtataaatta
     9001 gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga tgaactggaa
     9061 gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc gcgagccaat
     9121 ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct gaaaggcatt
     9181 gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt aatcgatggc
     9241 ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg ggttattggc
     9301 gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg cctgaaaacc
     9361 ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc ggcaacggtg
     9421 gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt gcaggcgatt
     9481 gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata catcgacatg
     9541 ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc gacaatcccg
     9601 gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagcgc agagatcgct
     9661 gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg taatacggtt
     9721 gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt aagcaaacaa
     9781 atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca gttgcaggca
     9841 ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt tctgtcatta
     9901 gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat tcgtgaacac
     9961 ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca gcgttttgtt
    10021 aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac cgacggcggc
    10081 cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc gcgtggccca
    10141 atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta caccattcgt
    10201 gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc attgacgcat
    10261 cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg tcctttcagg
    10321 gccgatatag ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt aggttcgact
    10381 cctattatcg gcaccattta aatcaataag ttacctcgca tttaagtaaa ccacgttctc
    10441 ctcttgtgcc gtatttgtgc cattgcgact tataatcgca tcgattttgc ttgcgtgctc
    10501 ggtgagatgc ccggctgaaa ggtgggcgta tctttgaacc atttcgagag tttcccatcc
    10561 tcccatctct ttaagtgcaa gaagagagac accggactga accagccagc ttgcccaggt
    10621 atgcctcaga tcatggaagc ggaagttgct aatgcctgcc cgctttaacg ctcccttcca
    10681 tgccttgttg ctgtcggttc tcatcttcct taccgctgct gtttttgttc cgtcgcttcg
    10741 gtaggcaggt ttggtgtgga caaataccca tctcttatgg agcccctgct gttttcttaa
    10801 tatctggcat gcggtttcgt taagaggaac tccgatcgca ttgccagctt ttgtttcatc
    10861 agggtgcatc catgccattt tcttatccag atcgacctgt gaccactcaa ggtctgtaac
    10921 gttggaacgg cgaaggcctg tcgtgattgc aaacatgacc acagggaaga agtgaggagc
    10981 aatttctgca aacaggcgct tcgattcctc ctctgtaagc catctgatgc gtccattctt
    11041 aacgcgtggt gttgatattt tgggtgccct gtcaagccat ccccattcaa cagccatatt
    11101 gagaatggcg cgaagtattg ccagatgcct cgtcttcgtt cctttgcttg caagctttgg
    11161 tttatactcc ggcaccggat tgccaagtcg caaacacctg tcccggctca tctcccagtt
    11221 caggcgatgg cggcggtttt ccatcccgtc taccgcctcc attatttttt ctgttgttat
    11281 gtcagagaga atggcttctc tgaagtgcaa catccagaac gatataatgc tcttgtcatc
    11341 atcaatggac ttcttatccg atttctcacg cagccaccgt atgcaggctt ccttgaatag
    11401 ctttttcggt gattccccga gattttttac tctccacgct ttgaagtggt caacaaaaac
    11461 tggccaccga gttagagttt ttccagtatc gattttccga ttcgtttggg ggtaacccac
    11521 cgttatattc gtgcggtctt agtgc
//
LOCUS       DAJTYG010000108        11318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTYG010000108 DAJTYG010000000
VERSION     DAJTYG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.108"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OO568_003282"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OO568_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR6642519.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="OO568_003283"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="OO568_003283"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6642520.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="OO568_003284"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="OO568_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6642521.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYDFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="OO568_003285"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="OO568_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6642522.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQL
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="OO568_003286"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="OO568_003286"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6642523.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="OO568_003287"
     CDS             6426..7055
                     /locus_tag="OO568_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6642524.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OO568_003288"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OO568_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR6642525.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTCILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OO568_003289"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OO568_003289"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6642526.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="OO568_003290"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="OO568_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6642527.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         2957 a         2916 c         2747 g         2698 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg acttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcta ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tccgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaccaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aattgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcac ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac cggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataaccg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgcag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcctggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgacg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctggct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggttat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtg
//
LOCUS       DAJTYG010000109        11272 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTYG010000109 DAJTYG010000000
VERSION     DAJTYG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.109"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="OO568_003291"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="OO568_003291"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR6642528.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /gene="malI"
                     /locus_tag="OO568_003292"
     CDS             complement(721..1749)
                     /gene="malI"
                     /locus_tag="OO568_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6642529.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /gene="malX"
                     /locus_tag="OO568_003293"
     CDS             1924..3516
                     /gene="malX"
                     /locus_tag="OO568_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6642530.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="OO568_003294"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="OO568_003294"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6642531.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4801..5802
                     /gene="add"
                     /locus_tag="OO568_003295"
     CDS             4801..5802
                     /gene="add"
                     /locus_tag="OO568_003295"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6642532.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5838..6878)
                     /locus_tag="OO568_003296"
     CDS             complement(5838..6878)
                     /locus_tag="OO568_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6642533.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7121..7246
                     /gene="blr"
                     /locus_tag="OO568_003297"
     CDS             7121..7246
                     /gene="blr"
                     /locus_tag="OO568_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6642534.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7519..7734
                     /gene="ydgT"
                     /locus_tag="OO568_003298"
     CDS             7519..7734
                     /gene="ydgT"
                     /locus_tag="OO568_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6642535.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7820..8260
                     /locus_tag="OO568_003299"
     CDS             7820..8260
                     /locus_tag="OO568_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6642536.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8337..8918
                     /gene="rsxA"
                     /locus_tag="OO568_003300"
     CDS             8337..8918
                     /gene="rsxA"
                     /locus_tag="OO568_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6642537.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8918..9496
                     /gene="rsxB"
                     /locus_tag="OO568_003301"
     CDS             8918..9496
                     /gene="rsxB"
                     /locus_tag="OO568_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6642538.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9489..>11272
                     /gene="rsxC"
                     /locus_tag="OO568_003302"
     CDS             9489..>11272
                     /gene="rsxC"
                     /locus_tag="OO568_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6642539.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAA"
BASE COUNT         2622 a         2911 c         2842 g         2897 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgccattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccaccgc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac ccagtttact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc
      421 aaatgcctga ccacccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttacc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctgc caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga cgtcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa tcgttgcgca atcgtggatt
     4741 tttaccctgc tttgttttta taatggtgcg cacttttata tccagaaaaa gagtgcgacc
     4801 atgattgata ccaccctgcc attaactgat atccatcgcc accttgatgg caacattcgt
     4861 ccccagacca ttcttgaact tggccgccag tataatatct cgcttcctgc acaatccctt
     4921 gaaacactga ttccccacgt tcaggtcatt gccaacgaac ccgatctggt gagctttctg
     4981 accaaacttg actggggcgt taaagttctc gcctctcttg atgcctgccg ccgcgtggca
     5041 tttgaaaaca ttgaagatgc agcccgtaac ggcctgcgct atgtcgagct gcgtttttca
     5101 ccaggctaca tggcaatggc acataagctg cctgtagcgg gtgttgtcga agcggtgatc
     5161 gatggcgtac gtgaaggttg ccgcaccttt ggtgtgcagg cgaagcttat cggcattatg
     5221 agccgaacct tcggcgaagc cgcctgtcag caagagctgg aggccttttt agcccaccgt
     5281 gaccagatta ccgcacttga tttagctggt gatgaacttg gattcccggg aagtctgttc
     5341 ctttctcact tcaaccgcgc gcgtgatgcg ggctggcata ttaccgtcca tgcaggcgaa
     5401 gctgccgggc cggaaagcat ctggcaggcg attcgtgaac tgggtgcgga gcgtattgga
     5461 catggcgtaa aagccattga agatcgggcg ctgatggatt ttctcgccga gcaacaaatt
     5521 ggtattgaat cctgtctgac ctccaatatt cagaccagca ccgtagcaga gctggctgca
     5581 catccgctga aaacgttcct tgagcatggc attcgtgcca gcattaacac tgacgatccc
     5641 ggcgtacagg gagtggatat cattcacgaa tataccgttg ccgcgccagc tgctgggtta
     5701 tcccgtgagc aaatccgcca ggcacagatt aatggtctgg aaatggcttt cctcagcgca
     5761 gaggaaaaac gcgcactgcg agaaaaagtc gccgcgaagt aacaaaaatg gatggtgcaa
     5821 attgcaccat ccatttttca cgcaaggcac aacgtcgcgc gatgtttggc ggattcgatg
     5881 cccagctcaa tcaactccat tacctggatt gcctggcttg ccggaaccgg attttcacca
     5941 tcgccattta acgcatcacg aatcgccgca tagtaagccg gataattccc aggcaccgtc
     6001 aacagcgttt cttcgacacg ttcctcacct tccacgcggg taagtacgcc atcacgcata
     6061 tcgtagcccc agtcttcctg cggcagacgc tcgccatttt tcagacgttc ttcctgtgga
     6121 tcgaggccat atttcacata actgcctcgg gatccatgca cgatataccg tgctgactca
     6181 gcggctgcca gcatggtacc gtgtaaaatg actcgccgct gtggatagga caagatggcg
     6241 tggaaataat cggtcgactg cgctccgggc cgtaactgtg ccaaatctac cgtcatgctg
     6301 accggtaaac caaatagcgt aatggcctga tcaagaagat gcggtgctaa atcgtaccag
     6361 ataccgctgc ctgggccgcc ctgttcacgc caacgatcgc gcacctgcgg acggaagcgg
     6421 tcaaaatgag actcaaagta agcaacttca cccagcacgc cttccgcgag caaacctttt
     6481 agcgtcaaga aatcgctatc ccaacgacgg ttatggaata cagacagcac acgccccagg
     6541 ctttttgcca gcgcatccag ctctcgcgct tgtgacagtg tcacggtaaa gggtttatca
     6601 acgaccacat gtttacccgc ctcaagcgcc gctttggcta acgggaaatg ggtatcgttg
     6661 ggtgtaggaa tgacaatcag gtctatgttg ggatcgttaa acagatgctt cggctcagag
     6721 acaaccgtaa ccgttggcca gtcggctttt acttttgttt catcactgct ggagattacc
     6781 gccagttcca gcccgggcgt gcccgcaatc aggggcgcat ggaaggtttt gctcgcataa
     6841 ccatacccaa tcaacccaac acggatgttg tcgctcatgt cattgcctct cattgaagta
     6901 tgatggctat ttgacactat cctttaccca cgctcaacag tttaataacc tgccagcaat
     6961 aagggatgtt gtttaactta agtcaaaaaa atagcgaatt ttccaacgac aaaagctaaa
     7021 tatcgcaaaa acctcagtaa aaatcttgct ggagctatta ttgctaagta acatttaccc
     7081 cctgaagtta atggatcaat caagagagat gtgggctgta atgaatcgtc ttattgaatt
     7141 aacaggttgg atcgttcttg tcgtttcagt cattcttctt ggcgtggcga gtcacattga
     7201 caactatcag ccacctgaac agagtgcttc ggtacaacac aagtaagctc tgcacttgtg
     7261 gagcgacatg ctgcccgtcc gggtgcatgt tttcacttgt cggatattaa accaggaatt
     7321 tattatcttg ttcgatgttg ttggtgattg tcagggatag taaagcgcgt tactcttttc
     7381 agagcagttt ttcatccctg aatatttgcc atcagcgcat tcttattatt gtaataacac
     7441 aagcgttcac ttttggtgaa attttatcat cactaataat acctacccct ttggtattaa
     7501 ttattttatg gaccttttat gactgttcag gactacttat taaaatttcg caaaatcagt
     7561 tcactcgaaa gtctggaaaa actctacgac catcttaatt acaccctgac ggacgatcag
     7621 gaactgatca atatgtatcg tgctgccgat caccgtcgcg cagagctggt ttctggcggg
     7681 cgtttgtttg acctcggcca ggtaccgaag tccgtctggc actatgtcca ataaagaaag
     7741 tagcgatcgg ttataaaagc cttataataa cgcccctaaa attagatttg cctgtttgcg
     7801 tcgctattct ggagaaccta tgaccaccac aacgccacaa cgtattggag gctggttgct
     7861 tggcccgttg gcctggcttt tagtcgctct gttgagtacg acgctggcgt tgttgctgta
     7921 taccgctgcg ttatcttctc cgcaaacatt tcaaacgctc ggcggacaag cactgacgac
     7981 acaaatctta tggggcgttt cttttattac cgctatcgcc atgtggtatt acacactgtg
     8041 gttgaccatc gctttcttta aacgtcggcg ttgtgttcct aaacactata ttatctggct
     8101 gcttatttcc gtattgctgg cagtaaaagc attcgctttt tcacccgtgg aagttggcat
     8161 agccgttcgc cagttgctgt ttactttgtt ggcaaccgca ttgatcgtcc cctatttcaa
     8221 acgctcgtcg cgggtaaaag cgacgtttgt gaatccgtaa taaccttaca gttaacctgt
     8281 tgtcgcctgc tctggattaa cggataatag gcggcttttt tatttcaggc cgaaaaatga
     8341 ctgactacct gttactgttt gtcggaactg tactggtcaa taactttgta ctggtcaagt
     8401 ttctcggtct ctgtccgttt atgggggttt ccaaaaaact ggaaaccgcg atgggcatgg
     8461 ggctggcaac aacgtttgtg atgacgctga cgtctatttg cgcctggctt atcgatacgt
     8521 ggattttgat cccacttaat ctgatttacc tacgcaccct ggcatttatt ctggtgattg
     8581 ctgtggtcgt gcagttcacc gagatggtgg tgcgcaaaac cagcccggtg ctttaccgtc
     8641 tgctggggat ttttttgccg cttatcacca ccaactgtgc agtgctcggc gtggcgttgc
     8701 tgaatatcaa tctcgggcac aatttcttgc agtcggcgct gtacggtttt tccgccgctg
     8761 tcggtttctc gctggtgatg gtgctcttcg ccgccatccg cgaacgcctt gctgtggctg
     8821 atgtcccggc accttttcgc ggtaatgcca ttgcgttaat taccgcaggt cttatgtctc
     8881 tggcctttat gggctttagt ggtttggtga agttgtaatg aatgctatct ggattgccgt
     8941 tgccgccgtg agcctgctgg gcctggcgtt tggcgccatt ctgggttatg cctcccgccg
     9001 ttttgcggtg gaagacgatc cggtcgttga gaaaattgac gaaatcttac cgcagagcca
     9061 gtgtggtcag tgcggttatc ccggctgtcg cccctacgcg gaagccatca gctgtaacgg
     9121 tgaaaaaatc aaccgttgcg ccccaggtgg cgaagctgtg atgctaaaaa ttgccgagtt
     9181 gcttaatgtc gagccgcagc cgctggatgg cgaagcgcaa gagctaacgc ctgcgcggat
     9241 ggtggcggtt attgatgaaa ataactgtat tggctgcact aaatgtattc aggcttgtcc
     9301 ggtagacgcc atcgttggcg ctacccgtgc catgcatacg gtaatgagtg atctctgtac
     9361 gggctgcaat ttatgtgttg atccgtgccc gacgcactgc atctcgttgc aaccggtcgc
     9421 agaaacacct gactcctgga aatgggatct gaacaccatt cccgtgcgta tcattcccgt
     9481 ggaacaccat gcttaagtta ttctctgcat tcagaaaaaa taaaatctgg gatttcaacg
     9541 gcggcatcca tccaccggag atgaaaaccc agtccaacgg tacccccctg cgccaggtac
     9601 cactggcgca gcgttttgtt attccactga aacagcatat tggcgctgaa ggtgagttgt
     9661 gcgttagcgt cggcgataaa gtattgcgcg gccagccgct tacccgtggt cgcggcaaaa
     9721 tgctgcctgt tcacgcgccc acctcgggta ccgttacggc tattgcgccc cactctacgg
     9781 ctcatccttc agctttagct gaattaagcg tgattattga tgccgatggt gaagactgct
     9841 ggatcccgcg cgacggctgg gtcgattatc gctctcgcag tcgcgaagag ttaatcgagc
     9901 gcatacatca gtttggtgtt gccgggctgg gcggtgccgg attcccgaca ggcgttaaat
     9961 tgcagggtgg tggagataag attgaaacgt tgattatcaa cgctgctgag tgcgagccgt
    10021 acattaccgc cgatgaccgt ttgatgcagg attgcgcggc tcaggtcgta gagggtattc
    10081 gcattcttgc gcatattctg cagccacgcg aaattcttat cggcattgaa gataacaaac
    10141 cgcaggcgat ttccatgctg cgcgcggtgc tggcggactc taacgatatt tctctgcggg
    10201 tgattccaac caaatatcct tctggcggtg ctaaacaatt aacctacatt ctgaccggga
    10261 agcaggttcc acatggcggg cgttcatccg atatcggcgt attaatgcaa aacgtcggca
    10321 ctgcttatgc agtgaaacgt gccgttattg atggcgagcc gattaccgag cgtgttgtaa
    10381 ccctgactgg cgaagcaatc gctcgcccgg gcaacgtctg ggcacggctg ggaacgccag
    10441 tgcgtcattt attgaatgat gccggattct gcccctctgc cgatcaaatg gtgattatgg
    10501 gtggcccgct aatgggcttt accttgccat ggctggatgt cccggtcgta aagattacca
    10561 actgtctgtt ggctccctct gccaatgaac ttggcgaacc acaggaagaa caaagctgca
    10621 tccggtgtag cgcctgtgct gacgcctgcc ctgtggatct tttgccgcaa cagttgtact
    10681 ggttcagcaa aggtcagcaa cacgataaag ctaccacgca taacattgcc gactgcattg
    10741 aatgtggggc ttgcgcgtgg gtttgcccga gcaatattcc cttggtgcaa tatttccgtc
    10801 aggaaaaagc tgaaattgcg gctattcgtc aggaagaaaa gcgcgccgca gaagccaaag
    10861 cgcgtttcga agcgcgccag gctcgtctgg agcgcgaaaa agctgctcgc cttgaacgac
    10921 ataagagcgc agccgttcaa cctgcagcca aagataaaga tgcgattgct gccgctctgg
    10981 cgcgggtgaa agagaaacag gcccaggcta cacagcctat tgtgattaaa gcgggcgaac
    11041 gcccggataa cagtgcaatt attgcagcac gggaagcccg taaagcgcaa gccagagcga
    11101 aacaggcaga actggagcaa actaacgacg cagcaaccgt tgctgaacca cgtaaaactg
    11161 ccgttgaagc agctatcgcc cgctccaaag cgcgcaagct ggaacagcaa caggctaatg
    11221 cggaaccaga agaacaggtc gatccgcgca aagccgccgt cgaagccgct at
//
LOCUS       DAJTYG010000110        11135 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTYG010000110 DAJTYG010000000
VERSION     DAJTYG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.110"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            373..2013
                     /gene="trg"
                     /locus_tag="OO568_003303"
     CDS             373..2013
                     /gene="trg"
                     /locus_tag="OO568_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6642540.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIGKIASGDLTMNDEPAGRNEIGRLSRHLQQIQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2051..2974)
                     /locus_tag="OO568_003304"
     CDS             complement(2051..2974)
                     /locus_tag="OO568_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6642541.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKESLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3191..4534
                     /locus_tag="OO568_003305"
                     /pseudo
     CDS             3191..4534
                     /locus_tag="OO568_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4759..6414
                     /gene="opgD"
                     /locus_tag="OO568_003306"
                     /gene_synonym="mdoD"
     CDS             4759..6414
                     /gene="opgD"
                     /locus_tag="OO568_003306"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6642542.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6554..6778
                     /locus_tag="OO568_003307"
     CDS             6554..6778
                     /locus_tag="OO568_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6642543.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6841..7377
                     /gene="rimL"
                     /locus_tag="OO568_003308"
     CDS             6841..7377
                     /gene="rimL"
                     /locus_tag="OO568_003308"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6642544.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7372..8352)
                     /locus_tag="OO568_003309"
     CDS             complement(7372..8352)
                     /locus_tag="OO568_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCR6642545.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8476..9468
                     /gene="tehA"
                     /locus_tag="OO568_003310"
     CDS             8476..9468
                     /gene="tehA"
                     /locus_tag="OO568_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6642546.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9465..10058
                     /gene="tehB"
                     /locus_tag="OO568_003311"
     CDS             9465..10058
                     /gene="tehB"
                     /locus_tag="OO568_003311"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6642547.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10361..11029
                     /locus_tag="OO568_003312"
     CDS             10361..11029
                     /locus_tag="OO568_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR6642548.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11047..>11135)
                     /locus_tag="OO568_003313"
     CDS             complement(11047..>11135)
                     /locus_tag="OO568_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6642549.1"
                     /translation="TPGQVENRALYPRPEGWGFTAHRIKSAI"
BASE COUNT         2871 a         2601 c         2802 g         2861 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagttgat agaaatatcg
       61 gtcggggcct tcgtctttct gattcccggt tagcctgaaa acagaaagtc tcaggcaccc
      121 gcaggcatcc tatgaggttt ccttagggac gaaaataatc acttcacgaa attgcgtgct
      181 gttttccaga atttttcgtc attcgggtta gccagtttag ccattcgtta ctctcttcat
      241 tccaatagca ttaattttct ctgcaataat tgttgtaaaa atgtgacgca aagaggtttt
      301 tggtcataag taattaccgt caagtgccga tgactttcta tcaggagtaa acctggacga
      361 gagacaacgg taatgaatac aactccctca cagcgattag gttttttgca tcacatcagg
      421 ctggttccgt tatttgcctg cattcttggc ggtatcttag ttctattcgc attaagttct
      481 gctctggctg gctatttcct ctggcaggcc gatcgcgatc agcgtgatgt tactgcggag
      541 attgagattc ggaccgggtt agcgaacagt tcagattttt tgcgttcagc ccggatcaat
      601 atgattcagg ccggggctgc gagtcgtatt gcggaaatgg aagcaatgaa gcgaaatatt
      661 gcgcaagccg aatcggagat taaacagtcg cagcaaggtt atcgtgctta tcagaatcga
      721 ccggtgaaaa cacctgctga tgaagccctc gacactgaat taaatcaacg ctttcaggct
      781 tatatcacgg gtatgcaacc tatgttgaaa tatgccaaaa atggcatgtt tgaagcgatt
      841 atcaatcatg aaagtgagca gatccgaccg ctggataatg cttataccga tattttgaac
      901 aaagccgtta agatacgtag caccagagcc aaccaactgg cggaactggc ccatcagcgc
      961 acccgcctgg gtgggatgtt catgattggc gcgtttgtgc ttgccctggt catgacgctg
     1021 ataacattta tggtgctacg tcggatcgtc attcgtccac tgcaacatgc cgcacaacgg
     1081 attggaaaaa tcgccagtgg cgatctgacg atgaatgatg aaccggcggg ccgtaatgaa
     1141 atcggtcgct taagtcgtca tttacagcaa atacagcatt cactggggat gacagtaggg
     1201 actgttcgac agggcgcgga agagatttat cgtggcacca gcgaaatttc agctggcaat
     1261 gcggacctgt catctcgcac cgaagaacaa gcggcggcta tcgaacaaac tgccgccagc
     1321 atggagcaac tcactgcgac ggtgaaacag aatgcggata acgcgcatca tgccagcaaa
     1381 ctggcgcaag aggcttctat taaagccagc gatggcgggc agacggtttc cggtgtagta
     1441 aaaacgatgg gcgctatctc cacgagttcg aagaaaattt ctgagatcac cgccgtcatc
     1501 aacagtattg ctttccagac gaatattctg gcactgaatg ctgccgttga agccgcgcga
     1561 gcgggtgagc aagggcgtgg atttgccgtt gtcgccagcg aagtacggac actcgcaagc
     1621 cgcagcgctc aggcagcgaa agagattgaa ggcttgatca gtgaatcagt caggttaatt
     1681 gacctggggt cggatgaggt ggcaacggca gggaaaacca tgagcactat tgttgatgcc
     1741 gtcgcgagtg tcacacatat catgcaggaa atcgccgccg cctcggatga acaaagtaga
     1801 ggcataactc aggttagcca ggcgatttct gaaatggata aggtgacgca acagaatgct
     1861 tctctggtag aagaggcctc agcagcggcg gtgtcccttg aagaacaggc tgcacgatta
     1921 actgaggcgg tgaacgtatt ccgtctgaac aaacattctg tgtcggcaga acctcgcgga
     1981 gcgggtgaac cagttagttt cgctacggtg tgaaaatgtt caaggaggga tcgacagatc
     2041 ccttcacctt tcagaacggc attgattttc gaatagcgtt aatcatcaac tggcaaccag
     2101 aagagaacgt cgcatctacg cgggtcagta ttccaatcgg ttcgcctgca ccatgtcccg
     2161 gaacaggcag ggccaccagc gtggcatgac gcaggtcgtc ttttacagcg ccagaaggga
     2221 caaaccacac gtaatcgtat tcaacggtaa gttgacgaga tagcgaagca gacagcgttt
     2281 cgatacaacc cgaaggaatt ttacagccct ggctctgcac taatgcatct gaatgctggc
     2341 gtggcgcagt gccttctggt gatacaacga ccggccattc cagcacccgg cttagcgtta
     2401 cgttctcctg aagtagcggg tgattagggc ggacaaccag cttcaacgat tcaagaaaca
     2461 gcagttcgta attaagcccg gtcatcagtt caggatctga catccgacca atgccgatat
     2521 cgatttcccc ggtttttaaa cccgccagaa tcatagggtt actcattgtc gcaacttgca
     2581 aggtcgtctc tttttgttgc tgatgaaact gacctataac cgaaggtaat atccccagtg
     2641 ccgcagtagg tagtgcacca accctgacga catcattatt aagactttct ttacgatgaa
     2701 gcgactgtcc ggcagtgttg atggcgtcaa gaactctgac tgcatgcgtt aaaaattgtt
     2761 cgccgggtaa ggtaagttgc gccccctgac gaccacgctc aaacaagcga gcgcccgtca
     2821 gctgctccag ttcattcaat gtattagaga gcgcaggttg actcaaatta agggtttcag
     2881 ctgcgcgccc caaagttcct tgttgtgcga cagctacgaa tgtatgaagg tggcgcaaac
     2941 ggatgcgctg actaaacaga ctattttttt ccataagcga tgttaaaaac gaagcggtgt
     3001 cgctgacaag tgaagttgtt tgattatgat aacttgattg taaaatatta ttaacaatta
     3061 aagcaattat gttacagcaa aatggataat attgatgttt tcgcggcgag atcacagttt
     3121 gtaaattctt cccgccagag tgaatgctgt tacctacact ccagattact gaccactgga
     3181 ggcagacact atggcgaaca gcatcacggc ggatgagatt cgggaacagt tttcgcaggc
     3241 aatgtcagcc atgtaccagc aagaagttcc gcaatatggc acgctgctgg aactggtagc
     3301 tgatgtgaat ctggctgtgc tggaaaacaa tcctcaactg cacgaaaaaa tggtaaatgc
     3361 agacgagctg gcgcgactga atgttgaacg tcatggggcg attcgcgttg ggactgcaca
     3421 agagcttgct actcttcggc ggatgtttgc cattatgggg atgtacccgg tgagctatta
     3481 cgatctctcg caggcagggg tgccggtaca ttcgacagca tttcggccca ttgatgatgc
     3541 ttctctggcg cgtaatccct tccgcgtttt tacatcctta ctccgccttg agcttatcga
     3601 gaacgaaatt ttgcgccaga aagcggcgga gattctgcgt cagcgcgata tcttcacccc
     3661 acgttgtcga caactgttag aggaatatga gcagcagggc ggttttaacg aaacacaggc
     3721 acaggagttt gtgcaggaag ccctggaaac gtttcgctgg cactagttag caacggtaga
     3781 tgaagaaacc tatcgcgcct tgcacaacga acatcggttg attgctgatg tggtctgttt
     3841 tcctggatgc catatcaacc acctgacgcc acgtacgctg gatattgacc gggtgcagtc
     3901 gatgatgcct gaatgcggaa ttgaacccaa aattctcatc gaggggccgc cgcgccgcga
     3961 ggtaccgatt ttactacgcc agaccagctt taaagcactg gaagagacgg tgttgtttgc
     4021 ggggcagaaa cagggcacgc ataccgcgcg ctttggtgaa attgagcagc gtggcgtggc
     4081 attaacgccg aaagggcgac aactgtatga tgatcttctg cgtaacgctg gaaccgggca
     4141 ggataatctc actcaccaaa tgcatttaca ggaaaccttc cgcacttttc ctgacagtga
     4201 gtttttaatg cgtcagcaag ggttggcatg gttccggtac cgtctgacgc cttcgggtga
     4261 ggcgcatcgt caggcgattc atcctggaga cgatccacag cccttaattg aacgtggttg
     4321 ggtagtggcg caacccatca cctatgaaga tttcttgccc gttagcgcgg cggggatctt
     4381 ccagtcaaat ctgggtaatg aaacgcagac acgcagtcac ggtaatgcca gtcgcgaagc
     4441 atttgagcag gcgttgggtt gtccggtttt ggatgagttc cagctttacc aagaagcgga
     4501 agaacgcagt aaacgtcgct gtggtttgct ttaaattctg accatccgcc tttgcaaaaa
     4561 tttgcctgat tttacaaacg aatcaggctc atcccatcga cataaaaaaa atgccgattt
     4621 atgcatattc tctcagttca acaattggat tattaataaa tattgtctag agtgagcggt
     4681 cataaataag cactttcttg ccgctgaaaa cgaccagcgc gggaccattc acaacaccag
     4741 aaggactcac tttcaggtat ggatcgtaga cgatttatta aaggttcaat ggctatggcc
     4801 gccgtgtgcg gtaccagcgg cattgcttct cttttttctc aggcagcatt cgcggcagat
     4861 tctgatattg ccgacgggca aacccagcgt tttgacttct ccattctaca gtcaatggcg
     4921 cacgacttag cgcaaacagc gtggcgtggt gcgcctcgtc cgttacctga cacgctggcg
     4981 acaatgacgc cgcaggctta taacagtatt caatacgacg ccgaaaaatc gctctggcat
     5041 aacgttgaga accgtcaact ggacgctcag ttcttccata tgggaatggg attccgtcgc
     5101 cgcgttcgta tgttttctgt agatccagca acacatctgg cgcgtgaaat tcactttcgc
     5161 ccggagttgt tcaaatacaa cgatgcaggt gttgatacca aacaattaga agggcaaagc
     5221 gatctcggct ttgccggttt tcgcgtgttt aaagcccccg aactggcgcg ccgtgatgta
     5281 gtatcatttc tcggcgcgag ttatttccgc gccgttgatg atacatatca atacggtttg
     5341 tcggcccgcg gcctggcgat cgacacttac accgacagta aagaagagtt ccccgacttt
     5401 accgccttct ggtttgatac ggtaaaaccg ggggcaacta cctttaccgt ttatgcgttg
     5461 ctcgatagcg ccagcattac tggtgcctat aagttcacta tccattgtga gaaaagtcag
     5521 gtgattatgg atgtggaaaa tcacctgtat gcgcgcaaag acattaaaca gctgggcatt
     5581 tcgccgatga ccagtatgtt cagctgcggt actaatgaac gtcggatgtg cgatacaatt
     5641 catccgcaaa ttcatgactc tgatcgtctg tccatgtggc ggggcaacgg cgagtggatt
     5701 tgccgtccgc tgaataatcc gcaaaaattg cagttcaatg cttacaccga caacaacccg
     5761 aaagggtttg gtttattgca actggatcgt gacttctccc attatcagga cattatgggc
     5821 tggtataaca aacgtccaag cctgtgggtg gaaccgcgta acaagtgggg taagggcacc
     5881 atcggcctga tggaaatccc aacaacgggc gaaacgctgg ataacattgt ctgcttctgg
     5941 cagccagaaa aagctgtaaa agcaggtgat gagtttgcat tccagtatcg tctgtactgg
     6001 agtgcgcaac cgcctgttca ttgcccatta gcgcgcgtta tggcgacgcg taccggcatg
     6061 ggcggtttcc cggaaggttg ggcgccaggt gaacactatc ccgaaaaatg ggcgcgtcgt
     6121 tttgccgtcg atttcgttgg tggtgatctg aaagctgccg cgccaaaagg cattgagccg
     6181 gtgattacgc tttccagtgg ggaagcgaag caaatcgaaa ttctctatat tgaacccatc
     6241 gatggttatc gtattcagtt tgactggtat ccgacttcgg actccactga tccggtcgat
     6301 atgcggatgt atctacgttg tcagggggac gctatcagtg aaacatggct gtatcagtat
     6361 ttcccgccag cgccggataa acgtcagtat gttgacgacc gcgtgatgag ttaatcgttt
     6421 tttcttcggc accttcttcg ggaggtgccg tctggttaaa cacgatcccg ctcgcatttt
     6481 tccctaagtt aaatgagtaa tctgatggtg tgtatttcag atacaccgtg tcagccacta
     6541 acagggagtg cgtatgtttc cagaataccg agatttaata tcccgtctga aaaacgaaaa
     6601 tcctcgcttt atgtccttgt tcgataaaca caataaactt gatcatgaaa ttgccagaaa
     6661 ggaaggttcc gacggtcgag ggtacaatgc ggaagtggtc cgcatgaaaa aacaaaagct
     6721 acagttaaaa gatgagatgc tcaaaatcct gcagcaggag agcgtcaaag aggtgtaaac
     6781 tttcctaagc cgccagccag gcggcttttt taacaacagc atggattgac tggagataag
     6841 atgactgaaa cgataaaagt aagcgaatca cttgaattac atgctgttgc ggaaagtcac
     6901 gtcacacctc tttatcagtt aatctgtaaa aataaaacct ggttacagca gtcgctaaac
     6961 tggccgcagt tcgttcagag tgaagaggac acgcgaaaaa cggtgcaggg taatgtgatg
     7021 ttgcatcaac gcggctatgc caaaatgttc atgattttca aagaagatga acttatcggc
     7081 gttatctcgt ttaatcgtat tgaaccactg aataaaactg ctgaaatcgg ctactggctg
     7141 gacgaatctc atcaggggca ggggatcatt tctcaggcgc tgcaggcatt gattcatcat
     7201 tacgctcagt ctggtgaact tagacgcttc gtgatcaaat gtcgggtgga caatccgcaa
     7261 agcaaccagg tcgctttgcg caatggtttt atccttgaag gttgcctgaa acaggctgag
     7321 ttcctgaatg atgcctatga tgatgtgaac ttatacgcgc gtattatcga ttcataataa
     7381 ccctgccagc ggcgttcgcg taatgcgctc ctcgccgtta atgacttttg gaccacggac
     7441 atgaaccgtg tcgccatcaa aggcttcaac gacggcgtgg tcggttaact ccacatggtt
     7501 ttcaattatc accgcacctg taatacggct ctcaccttgt attacaacgt gctcgtcgag
     7561 cagaatcggt ccaccacgta ccacggcatt tccgccaatc aagacatgat gttttaacac
     7621 acaattacct tccacaatgg catattccgc cacctgcgaa ctgtaatgaa tcgtcggaat
     7681 ggcatcttct tctatgccag ctttcacctg cgcgtgaccg tagactttag cgcaatcgca
     7741 tagccagacg ttgttctctt cattaccttc aacactggca aaatcaaaaa cttcggcgcg
     7801 atgttcaata aaagcatacc ggacgacggc atcgccataa atttgtgcct ggtggacaat
     7861 acgcgaggcg ctaacccttg cgcgatcata aatttggagc aggagttgat ggtcgggcgt
     7921 taagccttgt gcggcgacga tcatagaatg ttgatcaatt aaggcgtgac caaatattct
     7981 acattgcccg taaactaatg agtcatgaat ggtgacactg tcacttatat atgcaccctg
     8041 gctgatttca ctattatcga tccagacgtt atccgttgcg taaacctctc cccacagcac
     8101 gctggtgccg gtgatacgcg tattaccgga aatcaccgtt ccgccgaacg caatagcatt
     8161 ctggtcgtaa atccagcaat ttccttcctg tgctaacacc gtttcgcgat cgatccagcc
     8221 gccagcggtt cctgctatca catcgttaaa atcgctaatg gcgataatct gccgtaataa
     8281 cacacttttt ttagtgccat cttcctgata actaaaggct cgctgttctt cacttaggcg
     8341 atatttgcgc atagcgtttt cccacaggtg acttactata accgtagcaa attctgcggc
     8401 tctggctatg ctcgagaaaa ttccataaaa tgcatttcaa atatacttta taaattaaac
     8461 aaaatgagta agaagatgca gagcgataaa gtgctcaatt tgccggcagg ctactttggt
     8521 attgtgttgg ggacgatagg gatgggattt gcctggcgct atgccagcca ggtttggcag
     8581 gtcagccact ggttagggga tgggctggtg attctggcga tgatcatctg gggattattg
     8641 actagcgcat ttattacccg actcatacgc tttccgcata gcgtgctggc ggaagttcgc
     8701 catccagtgc tgagcagttt tgtgagtttg tttccggcaa cgacgatgct ggtggcgatt
     8761 ggttttgttc cgtggtttcg cccactggcg gtgtgcatgt tcagttttgg tgtcgtggtt
     8821 cagttggctt atgccgcctg gcaaactgcg ggattatggc gcggatctca ccctgaggaa
     8881 gctaccacgc ctggactgta tctgccgaca gttgccaaca actttatcag cgcaatggcc
     8941 tgtggtgcgt tgggctacac cgacgccggt ctggtgtttt taggcgcagg cgttttctca
     9001 tggctaagcc ttgaaccggt gatcttgcag cgtctgcgca gttcgggaga attacccacg
     9061 gcactgcgga catcactcgg cattcagctc gctcctgcgc tggtggcctg tagtgcctgg
     9121 ctgagcgtca acggcggcga gggtgacacg ctggcgaaaa tgcttttcgg ttatggactg
     9181 ctgcaactgc tgtttatgct acgtctgatg ccatggtatc tctcccagcc atttaatgct
     9241 tcattctgga gtttctcgtt cggcgtatct gcactggcaa ccaccggttt gcatctgggg
     9301 agtggcagcg ataatggatt tttccatacg ctggcggtgc cgctgtttat ctttaccaat
     9361 tttattattg caatactgct catccgtact tttgcgcttc tgatgcaggg aaaattgtta
     9421 gtcagaaccg agcgcgccgt tttaatgaaa gcagaggaca aagaatgatc attcgtgacg
     9481 aaaactattt tactgataaa tatgaattaa cccgcacaca ctctgaagta ctggaagcgg
     9541 tgaaagtggt taaaccgggt aaaacgctgg atctgggctg tggcaatggt cgtaacagtc
     9601 tttacctggc agccaatggt tatgatgttg acgcatggga taaaaatgcc atgagtatcg
     9661 ccaacgtcga gcgcattaaa tccattgaaa atctggataa tttacacacc cgggtcgttg
     9721 atctgaataa cctcacattt gatggacagt acgattttat tctttcgact gtggtgctga
     9781 tgttccttga ggctaaaacc atcccagggt tgattgccaa tatgcaacgt tgcactaaac
     9841 ctggtggtta caacttgatt gtggcggcga tggataccgc tgattatcca tgtaccgtcg
     9901 gcttcacgtt tgccttcaaa gagggagaat tacgtcgata ttacgaaggc tgggagaggg
     9961 tgaaatacaa tgaagacgtc ggcgagctgc accgcaccga cgccaacggt aatcgtatta
    10021 aactgcgttt cgccacgatg ctggcacgta aaaaatgacc cggtaagcac aaaacgcgtg
    10081 aaaattcccc acgctgagat gatttactgt tcttcttttc ggtaagcata ttttttatcg
    10141 aagggatgtg aaattaatca cagtagtcga agttttttag cagcttaact tactgaaatt
    10201 taactactga tgattgactt agcccctttt tcggcattga ctatgtcgtc tgaaaagggg
    10261 ctgaaaaatt tattttcacc aacacttttt ttgccacaac acgaagcggc gctttttgct
    10321 ataacttaga aagtaatata atcatctcag gaaactattc atgcgtacca catcatttgc
    10381 gaaagttgca gctttatgcg gcttgttggc tctgtctggt tgtgcctcta aaatcaccca
    10441 gccagataaa tattctggtt ttttaaacaa ttactctgat ttaaaagaaa caacctcagc
    10501 aacaggtaaa cctgttttac gttgggtaga cccgagtttt gatcaaagca aatatgacag
    10561 catcgtctgg aacccaatca cttattatcc ggtaccaaaa ccttctaccc aggtagggca
    10621 gaaagttctg gataaaattt tgaactatac caacaccgaa atgaaagaag cgatagcgca
    10681 gcgtaaacca gtggttacca ccgctgggcc gcgtagtctg attttccgtg gggccattac
    10741 cggtgtagat accagcaaag aagggctgca attctatgaa gtggttcctg ttgcattagt
    10801 ggttgcgggg acgcaaatgg ctacaggcca ccgtaccatg gatactcgcc tctattttga
    10861 aggtgagctg attgatgcag cgactaataa accggttatc aaagtcgttc gtcagggcga
    10921 aggtaaagac ctgaataacg aaagtacgcc aatggctttc gaaaatatta aacaagttat
    10981 tgatgacatg gcgaccgatg ccaccatgtt tgacgttaac aaaaagtagt tccagacgcg
    11041 ccatcgttag atggcgcttt ttatccggtg cgccgtaaaa ccccatcctt cagggcgggg
    11101 atataaggcg cggttttcca cctgaccagg tgttt
//
LOCUS       DAJTYG010000111        11091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTYG010000111 DAJTYG010000000
VERSION     DAJTYG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.111"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            213..2918
                     /gene="malT"
                     /locus_tag="OO568_003314"
     CDS             213..2918
                     /gene="malT"
                     /locus_tag="OO568_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCR6642550.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWNLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALVQQTEQIARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(2961..3838)
                     /gene="rtcA"
                     /locus_tag="OO568_003315"
                     /pseudo
     CDS             complement(2961..3838)
                     /gene="rtcA"
                     /locus_tag="OO568_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
     gene            complement(3842..5068)
                     /gene="rtcB"
                     /locus_tag="OO568_003316"
     CDS             complement(3842..5068)
                     /gene="rtcB"
                     /locus_tag="OO568_003316"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6642551.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5257..6855
                     /gene="rtcR"
                     /locus_tag="OO568_003317"
     CDS             5257..6855
                     /gene="rtcR"
                     /locus_tag="OO568_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR6642552.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGAQESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(6837..7595)
                     /locus_tag="OO568_003318"
     CDS             complement(6837..7595)
                     /locus_tag="OO568_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6642553.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7612..8442)
                     /gene="glpG"
                     /locus_tag="OO568_003319"
     CDS             complement(7612..8442)
                     /gene="glpG"
                     /locus_tag="OO568_003319"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6642554.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8487..8813)
                     /gene="glpE"
                     /locus_tag="OO568_003320"
     CDS             complement(8487..8813)
                     /gene="glpE"
                     /locus_tag="OO568_003320"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6642555.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9003..10508
                     /gene="glpD"
                     /locus_tag="OO568_003321"
     CDS             9003..10508
                     /gene="glpD"
                     /locus_tag="OO568_003321"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6642556.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSIGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(10777..11007)
                     /locus_tag="OO568_003322"
                     /pseudo
     CDS             complement(10777..11007)
                     /locus_tag="OO568_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         2679 a         3035 c         2840 g         2537 t
ORIGIN      
        1 tgaacataag ggaaaccagt attcacgctg gatcagcgtc gttttaggtg agttgttaat
       61 aaagatttgg aattgtgaca cagtgcaaat tcagacacat aaaaaaacgt catcgcttgc
      121 attagaaagg tttctggccg accttataac cattaattac gaagcgcaaa aaaaataata
      181 tttcctcatt ttccacagtg aagtgattaa ctatgctgat tccgtcaaaa ctaagtcgtc
      241 cggttcgact cgaccatacc gtggttcgtg agcgcctgct ggctaaactt tccggcgcga
      301 acaacttccg gctggcgctg atcacgagtc ctgcgggcta cggaaagacc accctcattt
      361 cccagtgggc ggcaggcaaa aacgatatcg gctggtactc gctggatgaa ggtgataacc
      421 agcaagagcg tttcgccagc tatctcattg ccgccgtgca gcaggcaacc aacggtcact
      481 gtgcgatatg tgagacgatg gcgcaaaaac ggcaatatgc cagcctgacg tcactcttcg
      541 cccagctttt cattgagctg gcggaatggc atagcccact ttatctggtc atcgatgact
      601 atcatctgat cactaatcca gtgatccacg agtcaatgcg cttctttatt cgccatcaac
      661 cagaaaatct caccctggtg gtgttgtcac gcaaccttcc gcaactgggc attgccaatc
      721 tgcgtgttcg tgaccaactg ctggaaattg gtagtcagca actggcattt acccatcagg
      781 aagcgaagca gttttttgat tgccgtctgt catcgccgat tgaagccgca gaaagcagtc
      841 ggatttgcga tgacgtttcc ggttgggcga cggcactaca gctaatcgcc ctctccgccc
      901 ggcagaatac ccactcagcc cataagtcgg cacgccgcct ggcgggaatc aatgccagcc
      961 atctttcgga ttatctggtc gatgaggttt tggataacgt cgatctcgca acgcgccatt
     1021 ttctgttgaa aagcgccatt ttgcgctcaa tgaacgatgc cctcatcacc cgtgtgaccg
     1081 gcgaagaaaa cgggcaaatg cgtctcgaag agattgagcg tcaggggctg tttttacagc
     1141 ggatggatga taccggcgag tggttctgct atcacccgct gtttggcaac ttcctgcgcc
     1201 agcgctgcca gtgggaactg gcggcggagc tgccggaaat ccaccgtgcc gccgcagaaa
     1261 gctggatggc ccagggattt cccagcgaag cgattcatca tgcgctggcg gcaggcgatg
     1321 cgctgatgct gcgcgatatt ctgcttaatc acgcctggaa tttgttcaac catagcgaac
     1381 tgtcgctgct ggaagagtcg cttaaggccc tgccgtggga cagtttgctg gaaaatccgc
     1441 agttagtgtt attgcaggcg tggctaatgc aaagccaaca tcgctatggc gaagttaaca
     1501 ccctgctagc ccgtgctgaa catgaaatca aggacatcag agaaggcacc atgcacgcag
     1561 aatttaacgc tctgcgcgcc caggtggcga ttaacgatgg taatccggat gaagcggaac
     1621 ggctggcaaa actggcactg gaagagctgc cgccgggctg gttctatagc cgcattgtgg
     1681 caacctcggt gctgggtgaa gtgctgcact gcaaaggcga attgacccgc tcactggcgc
     1741 tagtgcagca aaccgaacag atagcacgcc agcacgacgt ctggcactac gccttatgga
     1801 gtttaattca gcaaagtgaa attctgtttg cccaagggtt cctgcaaacc gcgtgggaaa
     1861 cgcaggaaaa agcgttccag ctgatcaacg agcagcatct ggaacagctg ccaatgcatg
     1921 agtttctggt gcgcatccga gcacaactgt tatgggcctg ggcacggctg gatgaagccg
     1981 aagcgtcggc gcgtagcggg attgaagtct tgtcgtctta tcagccacag caacagcttc
     2041 agtgcctggc aatgttgatt caatgctcgc tggcccgtgg tgatttagat aacgcccgta
     2101 gccagttgaa ccgtctggaa aacctgctgg ggaatggcaa atatcacagc gactggatct
     2161 ctaacgccaa caaagtccgg gtgatttact ggcaaatgac cggcgataaa gccgccgctg
     2221 ccaactggtt gcgtcatacg gctaaaccgg agtttgcgaa caaccacttc ctgcaaggtc
     2281 aatggcgcaa cattgcccgc gcacaaatct tgctgggcga gtttgaacct gcggaaattg
     2341 ttctcgaaga actcaatgaa aatgcccgca gtctgcggtt gatgagcgat ctcaaccgta
     2401 acctgttgct gcttaatcaa ctgtactggc aggccggacg taaaagtgac gcccagcgcg
     2461 tgttgctgga cgcattaaaa ctggcgaatc gcaccggatt tatcagtcat tttgtcatcg
     2521 aaggcgaagc gatggcgcaa caactgcgcc agctgattca acttaatacg ctgccggaac
     2581 tggaacagca tcgcgcgcag cgtattctgc gagaaatcaa tcaacatcat cggcataaat
     2641 tcgcccattt cgatgagaat ttcgttgaac gtctgctaaa tcatccggaa gtgccagaac
     2701 taatccgcac cagcccgttg acccaacgtg aatggcaggt cctggggctg atctactccg
     2761 gttacagcaa tgaacaaatt gcgggcgaac tggaagtggc ggcaaccacc atcaagacgc
     2821 atatccgcaa tctgtaccaa aaactcggcg tcgcccaccg tcaggctgcc gtgcagcacg
     2881 cgcagaaact gctgaagatg atgggttatg gggtgtgagt ttagccggat aatgcattgg
     2941 atccggttta catctttaca tcattcaata ctcacccgcg ttacgccatc tgtttctatc
     3001 aaaccaaacc gcaccggcaa gaaacgctcc accaccgcga tattggtcag cagatggcat
     3061 gaaggatggg cgaccgtaaa ttctcccgcg cccgccagcg ccatcggcag taccagttgg
     3121 tcggcgagat attcccccac cgccgctggg cttgccaggt agcgtttcac ctctttcact
     3181 aactgcgcgg ctaccacctc ggcactgacg cgcttttcac cgacgacgaa aaagcgttca
     3241 gtgatatttt cactttcgac ttccagcgag acggtattac ccggcccctg gtcgcgcggc
     3301 aggttatgaa tattctgttc atgcagggaa aaaatccccg ccagtgtagc gatttcacgc
     3361 tcagcaacat ggcgcggcac accagctaat agaacttctc cacgcatctg cacaatgttc
     3421 ccgcgctcgc caagttgcaa ggtgctaaac gatgccacgg gcgagacttc cgttgccacc
     3481 acaccgcctc cggcaggata aaaaccgtga cgtaacagcg tggtttgctg atgaattcct
     3541 attttcgcca gcagcggctc cagcacccgg cggataaaat ccgcaggcgg cgccgacggg
     3601 ttatcggtgc cgccgctcac ttcaacacgc gaaggtccat cggcaaacca cagcgcgggc
     3661 agcaccgttt gcagcaccag cgtacaactt ccggcgctac cgatagcaaa gcggtaatcg
     3721 ccgccgcgca cggtgccagg ccggaagacc agacgctgcg atcccagctc agcgccgagc
     3781 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
     3841 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
     3901 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
     3961 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
     4021 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
     4081 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
     4141 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
     4201 agtgctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
     4261 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
     4321 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
     4381 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
     4441 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
     4501 cacgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
     4561 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
     4621 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
     4681 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
     4741 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
     4801 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
     4861 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
     4921 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
     4981 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
     5041 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
     5101 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttttt aatttattgt
     5161 ttctaaaaag attatatctt tatgtccgta aacggagatt tcccgcaaag ccgatttacc
     5221 gataatgaaa tatcgccttt tataaggata tctaagatgc gtaaaacagt ggcttttggc
     5281 tttgtcggta ccgtgctgga ttatgccgga cgcggcagtc agcgctggtc aaaatggcgt
     5341 ccgtcactct gtatatgcca gcaagaatcg ttggtcatcg atcgactgga attattgcac
     5401 gatgcccgct cgcgctcgct atttgaaacg cttaaacgcg atatcgccag cgtttcgcca
     5461 gaaacagaag tggtgggcgt tgagattgaa ctgcataacc cgtgggattt cgaagaggtt
     5521 tacgcctgcc tgcatgattt cgcccgtggt tacgagtttc agccagaaaa agaagactat
     5581 ttaattcaca tcaccaccgg cacccacgtc gcgcagattt gctggtttct gctggcggaa
     5641 gcgcgttatc tacccgcccg gttgatacaa tcttcaactc cgcgcaaaaa agaacagccg
     5701 cgcggcccag gtgaagtgac gattatcgat ctcgatttaa gccgttataa cgccatcgcc
     5761 agccgctttg ccgaggaacg gcagcaaacg ctcgatttcc tcaaatccgg tattgccacg
     5821 cgtaaccccc acttcaaccg catgattgag cagatcgaaa aagtggcgat taaatcccgc
     5881 gcgccgattc tgcttaacgg cccgaccggc gcaggtaagt catttctggc gcgacgcatc
     5941 ttcgagttaa aacaggcacg gcatcagttt agcggcgcgt ttgtggaagt gaactgcgcc
     6001 accctgcgcg gcgataccgc gatgtcgacg ctgtttggtc atgtgaaagg cgcgtttacc
     6061 ggggcgcagg aatctcgtga aggattatta cgcagcgcca acggcggaat gttgtttctt
     6121 gatgagattg gcgaactggg cgcagacgaa caggcaatgc tgctgaaagc tattgaagag
     6181 aaaacctttt acccgtttgg cagcgatcgc caggtgagca gtgattttca gcttatcgcc
     6241 ggaacggtgc gcgatttgcg ccagctggtt gccgaaggca aattccgcga agatctgtac
     6301 gcgcggatca atctctggac cttcaccctg ccgggtctac gccagcgcca ggaagatatt
     6361 gaaccgaacc tggattatga agtggagcgc cacgccacac tcactggtga cagcgtgcgt
     6421 tttaacaccg aagcgcggcg tgcctggctg gcctttgcaa catctcccca ggctacatgg
     6481 cgcggtaact ttcgcgaact ttctgccagc gtcacgcgga tggccacctt tgccactagc
     6541 ggacgcatca ctctggacgt ggtggaagat gagataaacc gtctgcgcta taactggcag
     6601 gagagccgcc cctccgcgct tacggcgttg ctgggcgcag aggcagaaaa catcgatctc
     6661 ttcgaccgta tgcaactgga acacgttatc gctatctgcc gccaggcaaa gtcgctttct
     6721 gctgccggac gacagctttt tgatgtttcg cgccagggca aagccagcgt caatgacgcg
     6781 gatcggctac gcaaatacct ggcgcgtttt ggtctgacgt gggaggccgt gcaggatcag
     6841 cacagctcca gttgaatatg gtggtccgtc agcacctgca tcacgcttac tggcggcggg
     6901 gcgtcggtgt agacggcatc aaccatgctg atgctcccca tattgaccat cgcattacgg
     6961 ccaaattttg aatggtcaac caccagcata acgtggcgcg agttctcaat aatggcgcgc
     7021 ttggtacgaa cttcgtggta atcgaactcc agcagcgagc cgtcgctgtc gatgccgctg
     7081 atccccagaa tgccgaaatc aaggcggaac tgggagataa aatcgagcgt cgcttcgcca
     7141 atgatcccgc catcgcggct acgtaattcg ccaccggcga gaataatgcg gaaatcttct
     7201 tttaccatca acgtgttagc aacgttgaga ttgttggtga caatgcgcaa attgctgtga
     7261 ttgagcagcg cgtgcgccac cgcttccggc gtggtgccga tatcgataaa cagcgtcgag
     7321 ccattgggga tttgctccgc cactttgcgg gcgatgcgct ctttttcttc ggtctgggtg
     7381 gctttacgat cgtgccacgg cgtgttaacc gaactggaag gcagcgccgc gccgccatga
     7441 tggcgcagga tcaggttttg ctctgccagc tcgttgaggt cgcggcgaat agtctgcggg
     7501 ctgacggaga agtgctccac cagttcctcg gtactgacat aaccctgctg tttaaccagt
     7561 tcgataatac cgttgtgacg ttgtgtttgt ttcatttata aatccctgga attattttcg
     7621 ttttcgcgca ttgagcgaat caacaaaagc catcgctaaa cccacggcta atccggcgat
     7681 gtgtgctccg ttcgccatcg acatcccaaa caaatcaaac catccggcga caatccagat
     7741 cagcgcaaag ataattaacc cacgttgcag gtaaatgcca ctttgcggat cgcgttcgcc
     7801 acgtagccag acgtagccca tcagcgcata caccacgcca gaaagcccgc caaaccacgg
     7861 cccgctgaat ttttgctgca catagccgct taacagggcg ctgataagcg taatgacaat
     7921 tagcttaccg ctaccgaggc gtttttccac cgcgccgccg agataccacc accaaagcag
     7981 gttaaagagg atatgcatca gcgagaagtg cattaacgcg tgggtgaagt aacgccagaa
     8041 ctcaaatttc agtgttggat cgaatggcca ggccagccat aacatcactt cctgatcgcc
     8101 gagaatttgc atggcaataa acaccaccac gcaggcgatc atcatcaccc aggtcaccgg
     8161 acctgcgcgt tcacgcaagg cagcaaagaa aggataacgg cgataatgca ggccactgcc
     8221 ggtatggcct gccagccagc tggccgccag ataacgcgga tctgccgggt tttcgagaaa
     8281 acgcgccagc tccgcccgca cgcgctcggc ctgggactca tccgccagcc agacatcgct
     8341 ttggttatgt tgttgaatcg tgaggataac accctgcgtc gccatgtaat caacaaacgc
     8401 ctgcgccacg cgggggttag caaaagaggt aatcatcaac atcgttgctg tcgcttattc
     8461 cacacaaaag gggacagtat aaagcgttac gcgccgtacg ccacctctgc gggaaactgt
     8521 cgctgccagg cttcaaagcc gccgtcaatg ctatagacca catcgtagcc ctgttgcagc
     8581 agatactgcg ccgccccttt gctgctattg ccgtgataac acatcaccat caccggagtg
     8641 tcaaagtcgt tatcacgcat aaacgcgccc agcgtgtcgt tggttaaatg gaaagcctgc
     8701 accgcatgcc ccatagcgaa actctgtgga tcgcgaatat cgaccagcac cgcctctttt
     8761 tcctgcaact tctggtgcgc gtcggcaacg ttaatacatt cgaactgatc catgcgtctc
     8821 tctttcttta caaacaagtg ggcaaattta ccgcacagtt tacgtcgaag cggcagataa
     8881 acgccataat gttatacata tcactctaaa atgttttttc aatgttaccc aaagcgcgat
     8941 tctttgctaa tatgttcgat aacgaacatt tatgagcttt aacgaaagtg aatgagggca
     9001 gcatggaaac caaagatctg attgtgatag gtggcggcat caatggtgct ggtatcgcgg
     9061 cagacgccgc tggacgcggt ttatccgtgc tgatgctgga ggcgcaggat ctcgcttgcg
     9121 cgacctcttc cgccagttca aaactcattc acggtggcct gcgctacctt gagcactatg
     9181 aattccgcct ggtcagcgag gcgctggctg aacgtgaagt gctgctgaaa atggctccgc
     9241 atatcgcctt cccgatgcgt tttcgcctgc cacatcgtcc gcatctgcgc ccggcgtgga
     9301 tgattcgcat tggtctgttt atgtacgatc atctgggtaa acgcaccagc ctgccgggat
     9361 caattggttt gcgttttggc gcaaattcag tgttaaaacc ggaaattaag cgcggattcg
     9421 aatattctga ctgttgggta gacgacgccc gtctggtact cgccaacgcc cagatggtgg
     9481 tgcgtaaagg cggcgaagtg cttactcgga ctcgcgccac ctctgctcgc cgcgaaaacg
     9541 gcctgtggat tgtggaagcg gaagatatcg ataccggcaa aaaatatagc tggcaagcgc
     9601 gcggcctggt taacgccacc ggcccgtggg tgaaacagtt cttcgacgag gggatgcatc
     9661 tgccttcgcc ttatggcatt cgcctgatca aaggcagcca tattgtggtg ccgcgcgtgc
     9721 atacccaaaa gcaggcctac attctgcaaa acgaagataa acgcattgtg ttcgtgatcc
     9781 cgtggatgga cgagttttcc atcatcggca ccaccgatgt ggaatacaaa ggtgatccga
     9841 aagcagtgaa gattgaagag agtgaaatca attacctgct gaaagtgtat aacgcgcact
     9901 ttaaaaagca gctaagccgt gacgatatcg tctggaccta ctccggtgtg cgtccgctgt
     9961 gtgatgatga gtccgactcg ccgcaagcca ttacccgtga ttacaccctt gatatccatg
    10021 atgaaaacgg caaagcaccg ctgctgtcgg tattcggcgg taagctgacc acctaccgaa
    10081 aactggcaga acatgcgctg gaaaaactga cgccgtatta tcagggtatt ggcccggcgt
    10141 ggacgaaaga gagcgtgctg ccgggtggtg ccattgaagg cgaccgcgac gattacgccg
    10201 ctcgcctgcg ccgccgttac ccgttcctga ctgaatcgct ggcacgtcac tacgcgcgca
    10261 cttacggcag caacagcgag ctgctgctcg gcaatgcggg aacggtaagc gatctcgggg
    10321 aagatttcgg tcatgagttc tacgaagcgg aactgaaata cctggtggat cacgaatggg
    10381 tccgccgcac cgacgatgcc ctgtggcgtc gcacaaaaca aggcatgtgg ctgaatgcgg
    10441 atcaacaatc tcgtgtgagt cagtggctgg tggaatatac gcagcagaag ttgtcgctgg
    10501 cgtcgtaaat taacgtaagg tgatcaggtc agatttcaat ctggcctgag actgatgaca
    10561 aacacaaaac tgcctgatgc gctacgctta tcaggcctac gtggtttatg caatatattg
    10621 aatttgcatg gtcttgtagg ccagataaga cgttcacgtc gcatccggca tgaacaaagc
    10681 gcactttgtc aaaaatctaa cctacttttt aattcaggga attaccgcaa agcccacgcc
    10741 atcataccca acgtaacaaa actcaggcac gttccccact cgccccgaga aaaatagcat
    10801 taatgcgccc agcgtcagca taaaaatatt gagcagaagg taaattgttg gcgtaaagag
    10861 taaattgcaa acctcaggaa tagcggcaag cccagccccc agcatggcaa taaccgctgc
    10921 aaaaaattgc gtcagtattg ggatgccgaa aaaagtcatt accagcgagg tcaaaatcca
    10981 ttttctattt tgcattattc tttccattct ttctgaatgg cgaaattata ctccccttgc
    11041 cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg g
//
LOCUS       DAJTYG010000112        10800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTYG010000112 DAJTYG010000000
VERSION     DAJTYG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.112"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            84..1169
                     /locus_tag="OO568_003323"
                     /pseudo
     CDS             84..1169
                     /locus_tag="OO568_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            1246..1854
                     /locus_tag="OO568_003324"
     CDS             1246..1854
                     /locus_tag="OO568_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6642557.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1952..3343
                     /gene="selA"
                     /locus_tag="OO568_003325"
     CDS             1952..3343
                     /gene="selA"
                     /locus_tag="OO568_003325"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6642558.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3340..5184
                     /gene="selB"
                     /locus_tag="OO568_003326"
     CDS             3340..5184
                     /gene="selB"
                     /locus_tag="OO568_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6642559.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPER"
     gene            5374..6525
                     /gene="yiaY"
                     /locus_tag="OO568_003327"
     CDS             5374..6525
                     /gene="yiaY"
                     /locus_tag="OO568_003327"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR6642560.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            6690..8228
                     /gene="aldB"
                     /locus_tag="OO568_003328"
     CDS             6690..8228
                     /gene="aldB"
                     /locus_tag="OO568_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR6642561.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRCIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(8447..8590)
                     /locus_tag="OO568_003329"
     CDS             complement(8447..8590)
                     /locus_tag="OO568_003329"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642562.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            8773..9096
                     /locus_tag="OO568_003330"
     CDS             8773..9096
                     /locus_tag="OO568_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6642563.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLISKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            9102..10238
                     /locus_tag="OO568_003331"
     CDS             9102..10238
                     /locus_tag="OO568_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6642564.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQVR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(10235..>10800)
                     /locus_tag="OO568_003332"
     CDS             complement(10235..>10800)
                     /locus_tag="OO568_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6642565.1"
                     /translation="FYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYASQRYL
                     EKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNTQSAL
                     ELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRVQYVL
                     DEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         2571 a         2651 c         2976 g         2602 t
ORIGIN      
        1 ctttctgacc gaatttcccg gctggagcat caactcgccg ccgagaaaaa gactgactat
       61 tccacgttcc cggagatcta attatggatc tattgattgt tttaacttac gtggcgctgg
      121 cgacggcggc gctgggaggc gtgtttctgg tgagtggttt gattttgttg atgaactaca
      181 accaccctta cacttttacc gcgcaaaagg cagtgatagc gatccctatc acgccacagg
      241 tgacgggaat tgttactgaa gtcactgaca agaataatca gcttattcaa aagggcgagg
      301 tgctttttaa gctcgacccg gttcgttacc aggcgcgagt tgacagactt caggctgacc
      361 tgatgacggc gacgcataat ataaagacgc tgcgtgcgca gctcactgaa gcgcaggcca
      421 acaccaccca ggtttcagcg gagcgcgacc gtctgtttaa aaattatcaa cgttacctga
      481 aaggcagcca ggcggcggtg aatccgttct cggaacgtga catcgacgat gcgcggcaaa
      541 atttcctcgc gcaggatgcg ttagtgaaag gctcggtggc ggagcaggcg cagatccaga
      601 gtcagctcga cagtatggtc aacggcgagc aatcgcagat tgtgagctta agagcgcaac
      661 ttactgaagc aaaatataat cttgagcaga ctgtcattcg cgcaccaagc aatggctacg
      721 tcacttaggt actgatccgc ccaggtacat acgcagctgc cttgccgctg cgtccggtga
      781 tggtcttcat ccccgagcaa aaacggcaaa ttgtcgccca atttcggcaa aactcgctgt
      841 tacgtctgaa acctggcgat gatgcggaag tggtgtttaa cgcgctacct gggcaggtgt
      901 tccacggcaa attgactagt attttacctg tcgtgccagg cggttcttat caggcgcagg
      961 gggtattgca atcattaacg gtcgttcccg gcacggacgg tgtgctggga accattgaac
     1021 tggaccctaa cgatgatatc gatgccttac ccgacggcat ctgcgcccag gtggcggttt
     1081 actccgacca tttcagccat gtttcggtga tgcggaaagt gctgctaaga atgaccagtt
     1141 ggatgcatta tctttatttg gatcattgag acttatatca cctgtgtttt gcgtgagcct
     1201 gagcctgagc catactgata tttttcctcg cacaaaggac tcggcatgaa actcgtcggt
     1261 agctacacca gcccgtttgt acgcaaactt tctattctgt tgttagaaaa gggcataact
     1321 ttcgaattta ttaatgaact gccctataac gcggataacg gcgtggcgca atttaacccg
     1381 ttagggaaag tgccggtgct ggtgaccgaa gagggcgaat gctggtttga ttcgccgatc
     1441 atcgctgaat atattgagtt aatgaatgtc gctccggcga tgttgccgcg cgatccgctg
     1501 gagtcgttgc gggtgcgcaa aattgaggcg ctggcggatg gcattatgga tgccgggctg
     1561 gtatcggtgc gtgaacaggc gcgtccggcg gcgcagcagt ctgaagatga attgttacgc
     1621 cagcgggaga aaatcaaccg cagtctggat gtgctggaag gatatctggt cgatggcaca
     1681 ctcaaaaccg atacggtcaa tctggcgact atcgccattg cctgtgctgt cggatatctc
     1741 aatttccgcc gcgttgcgcc aggttggtgt gtcgaccgcc cacacttagt caaactggtc
     1801 gaaaacctgt ttagccgcga aagttttgct cgcaccgaac cgccaaaggc ttgatgcgcg
     1861 atatgtcctc ctgacccatc tcacgttaca atccgtggtt atgttaaacg cccttctccg
     1921 tgtgagaggg ccttgatcag ccaggtttcc tatgacaacc gaaacgcgtt ccctctatag
     1981 tcaacttccg gctattgatc gcttattacg cgatagctcc ttcctttctt tgcgtgatac
     2041 ttatggtcac acccgcgtgg tggaattgtt gcgtcagatg ctcgacgaag cgcgagaagt
     2101 gattcgtggc agccagacgc tgcctgcgtg gtgtgaaaac tgggcgcaag aagtcgatgc
     2161 ccggttgacg aaagaggcgc agagcgcgct gcgtccggtg atcaacctga cgggaaccgt
     2221 gctgcatacc aaccttgggc gagctttaca ggcggaagcc gcggtggaag ccgttgcgca
     2281 ggctatgcgt tcgccagtga ccctcgagta cgatctggac gacgccggac gcggacatcg
     2341 cgatcgggcg ctggcgcagc tgctgtgccg tattacgggg gcggaagatg cctgtatcgt
     2401 caataacaat gcggcggcgg tgttattgat gttggcggcc actgccagcg ggaaagaggt
     2461 ggtggtatct cgcggcgaac tggtggagat tggcggcgcg tttcgtattc ccgatgttat
     2521 gcgtcaggca ggctgcaccc tacacgaagt agggaccacc aaccgcacgc acgcgaatga
     2581 ttatcgtcag gcggtgaatg aaaataccgc actgttgatg aaagtacata ccagtaacta
     2641 cagcattcag gggttcacca aagcgataga cgaagctgaa ctggtggcgc tcggcaaaga
     2701 gctggatgtt ccagtggtga ctgatttagg cagtggctcg ctggtcgatc ttagccagta
     2761 cggtttgccg aaagagccaa tgccgcagga gttgattgcg gcgggcgtca gtctggtgag
     2821 cttctccggc gacaagttgt taggcgggcc gcaggcaggg attatcgttg gtaaaaaaga
     2881 gatgatcgcc cgcctgcaaa gccacccgct gaagcgtgca ttacgcgcgg ataaaatgac
     2941 cctcgcggcg ctggaagcca cgttgcgtct ttatttacac cctgaagctc tgagtgaaaa
     3001 attaccgacc ctgcgcctgc ttacccgcag cgcagaggtc attcaaatcc aggcacaacg
     3061 tttacaggct ccccttgtcg cacattacgg cgcggagttt gcggtacagg ttatgccatg
     3121 tctttcgcag attggcagtg gttcgctgcc ggttgatcgc ctgccgagcg cggcattaac
     3181 gtttacaccc catgatggac gcggtagcca ccttgagtca ttagccgccc gctggcgtga
     3241 attgccagtg ccggtgattg gtcgtattta tgacggacga ttgtggctgg atttacgctg
     3301 ccttgaagat gagcaacggt ttttggagat gttgttgaaa tgattattgc gactgccgga
     3361 cacgttgacc acggcaaaac aaccttattg caggcgatta ctggcgtaaa tgctgaccgt
     3421 ctgccggaag aaaaaaagcg cggcatgacc atcgatctcg gctatgccta ctggccgcag
     3481 ccggatggtc gcgtgcctgg ttttatcgac gttcccggtc atgaaaagtt tctttccaac
     3541 atgctggcgg gcgttggtgg tatcgatcac gcgctgttgg tggtggcgtg cgatgacggc
     3601 gtgatggcac agacccgtga gcatctggcg attttgcagc tgaccggtaa cccgatgctg
     3661 acagtggcgc tgaccaaagc cgatcgtgtg gacgaagcgc gtgttgatga ggttgaacgc
     3721 caggtaaagg aggttctgcg ggaatacggt tttgctgagg caaaactgtt tatcaccgca
     3781 gcaaccgaag gtcggggaat tgatgccctg cgcgagcatc tgcttcagtt gccggaacgc
     3841 gagcacgcca gccaacatag tttccgcctc gcgattgacc gcgcatttac cgtaaaaggt
     3901 gccgggctgg tcgtcaccgg tacggcgtta agcggggaag tgaaggtagg cgattcactc
     3961 tggctgactg gtgtaaataa accgatgcgt gtacgtgcgc tgcatgcgca aaaccagcca
     4021 acagaaaccg ccaatgccgg gcagcgtatc gcgcttgaca tcgcgggtga tgcggaaaaa
     4081 gagcagatta accgtggcga ctggctgctt gccgatgtgc cgccagagcc gttcacacgg
     4141 gtgattgtcg agcttcaaac ccatgcaccg ctgacccagt ggcagccgct gcatattcac
     4201 cacgccgcca gccacgtcac gggacgtgtt tcactgctgg aagataacct tgctgaactg
     4261 gtcttcgaca ccccgttatg gctggcagat aacgaccgcc tggtattgcg cgatatctct
     4321 gcccgcaaca cgctggccgg agcgcgcgtc gtgatgctta acccgccgcg tcgcggtaaa
     4381 cgtaaaccag agtatctgca atggctggct tccctcgcca gggcacagaa tgatagcgaa
     4441 gcgttagccg ttcacctgca acgcggcgcg gttaatcttc ctgatttcgc ctgggcgcgc
     4501 cagctcaacg gcgaagggat gcgcgaactg ctgcaacagc ctggctatat tcaggctggt
     4561 tatagcttgt tgaatgcgcc ggttgccgct cgctggcaac ggaaaattct cgacacatta
     4621 gcgacttatc atgagcaaca tcgcgatgaa cctgggcctg ggcgcgaacg tctgcgacgt
     4681 atggcgttgc caatggaaga tgaagcgcta gtactgttgc tgattgaaaa gatgcgcgaa
     4741 agcggcgaca tcctcagtca tcacggttgg ctgcatctgc cggatcacaa agcgggcttc
     4801 agcgaagagc agcaggccat ctggcaaaaa gcagagccgc tgtttggtga cgagccgtgg
     4861 tgggtgcgtg atctggcaaa agagacgggt accgacgagc aggcaatgcg cctgacgcta
     4921 cgccaggcgg cgcagcaagg aataattacc gcgatcgtca aagatcgtta ttaccgtaac
     4981 gatcggattg tcgaatttgc caatatgatc cgcgatctcg atcaggagtg tggttcaacc
     5041 tgcgcggcgg atttccgcga tcgcttaggc gtaggccgaa agctggcaat tcagattctg
     5101 gaatattttg accgcattgg ctttacgcgt cgtcgtggaa atgatcattt attgcgcgac
     5161 gcattattat ttccggaaag ataaggaaat gattaattaa tttttaaaat aaattaatac
     5221 aaatttctta tgattttaaa taaagcacat tgtttaatga atacaatgtg ctttttatta
     5281 gattaatttt gataattaat aataatgatc atgttttcga atgaaaatcc tcagtaagct
     5341 gcccaccctt ttttacactt tcaggagtgt gttatggcat cttcaacttt ctttattcct
     5401 tctgtgaatg tcatcggcgc tgattcattg actgatgcaa tgaatatgat ggcagattat
     5461 ggatttaccc gtaccttaat tgtcactgac aatatgttaa cgaaattagg tatggcgggt
     5521 gatgtgcaaa aagcactgga agaacgcaat atttttagcg ttatttatga tggcacccaa
     5581 cctaacccca ccacggaaaa cgtcgccgca ggtttgaaat tacttaaaga aaataattgc
     5641 gatagcgtga tctccttagg cggtggttct ccacacgact gcgcaaaagg tattgcgctg
     5701 gtggcagcca atggcggcga tattcgcgat tacgaaggcg ttgaccgctc tgcaaaaccg
     5761 cagctgccga tgatcgccat caataccacg gcgggtacgg cctctgaaat gacccgtttc
     5821 tgcatcatca ctgacgaagc gcgtcataac aaaatggcga ttgttgataa gcacgtgact
     5881 ccgctgcttt ctgtcaatga ctcctctctg atgattggta tgccgaagtc actgaccgcc
     5941 gcaacgggta tggatgcctt aacgcacgct atcgaagcgt atgtttctat tgccgccacg
     6001 ccgatcactg acgcttgtgc actgaaagcc gtgaccatga ttgccgaaaa cctgccgtta
     6061 gccgttgaag atggcagtaa tgcgaaagcg cgtgaagcaa tggcttatgc ccagttcctt
     6121 gccggtatgg cgttcaataa tgcttctctg ggttatgttc atgcgatggc gcaccagctg
     6181 ggcggtttct acaacctgcc acacggtgta tgtaacgccg ttctgctgcc gcatgttcag
     6241 gtattcaaca gcaaagtcgc cgctgcacgt ctgcgtgact gtgccgctgc aatgggcgtg
     6301 aacgtgacag gtaaaaacga cgcggaaggt gctgaagcct gcattaacgc catccgtgaa
     6361 ctggcgaaga aagtggatat cccggcaggc ctacgcgacc tgaacgtgaa agaagaagat
     6421 ttcgcggttc tggcgactaa tgccctgaaa gatgcctgtg gctttactaa tccgatccag
     6481 gcaactcacg aagaaattgt ggtgatttat cgcgccgcaa tgtaatcatc attccaacaa
     6541 cggctggcaa attgtcagcc gctttttcaa ctatctctgt aatccttgcc cgtaaattcg
     6601 tgatagctgt cgtaaagctg ttaccgactg gcgaagattt cgccagtcac gtctaccctt
     6661 gttatacctc acaccgcaag gagacgatca tgaccaataa tcccccttca gcacagatta
     6721 agcccggcga gtatggtttc cccatcaagt taaaagcccg ctacgacaac tttattggcg
     6781 gtgaatgggt agcccctgcc gatggtgagt attaccagaa cctgacgccg gtgaccgggc
     6841 agctgctgtg cgaagtggcg tcttcgggca aacgagacat cgatctggcg ctggatgctg
     6901 cgcacaaagt gaaagataaa tgggcgcaca cctcggtgca ggatcgcgcg gcgattctgt
     6961 ttaagattgc cgatcgaatg gaacaaaacc tcgagctgtt agcgacagct gaaacctggg
     7021 ataacggcaa acccattcgc gaaaccagtg ctgcggatgt accgctggcg attgaccatt
     7081 tccgctattt cgcctcgtgt attcgggcgc aggaaggtgg gatcagtgaa gttgatagcg
     7141 aaaccgtggc ctatcatttc catgaaccgt taggcgtggt ggggcagatt atcccgtgga
     7201 acttcccgct gctgatggcg agctggaaaa tggctcccgc gctggcggcg ggcaactgtg
     7261 tggtgctgaa acccgcacgt cttaccccgc tttctgtact gctgctaatg gaaattgtcg
     7321 gtgatttact gccgccgggc gtggtgaacg tggtcaatgg cgcaggtggg gaaattggcg
     7381 aatatctggc gacctcgaaa cgcatcgcca aagtggcgtt taccggctca acggaagtgg
     7441 gccaacaaat tatgcaatac gcaacgcaaa acattattcc ggtgacgctg gagttgggcg
     7501 gtaagtcgcc aaatatcttc tttgctgatg tgatggatga agaagatgcc tttttcgata
     7561 aagcgctgga aggctttgca ctgtttgcct ttaaccaggg cgaagtttgc acctgtccga
     7621 gtcgtgcctt agtgcaggag tcaatctacg aacgctttat ggaacgcgcc atccgccgtg
     7681 tcgaaagcat tcgcagcggt aacccgctcg acagcgtgac gcaaatgggc gcgcaggttt
     7741 ctcacgggca actggaaacc atcctcaact acattgatat cggtaaaaaa gagggcgctg
     7801 acgtgctcac aggcgggcgg cgcaagctgc tggaaggtga actgaaagac ggctactacc
     7861 tcgaaccgac gattctgttt ggtcagaaca atatgcgggt gttccaggag gagatttttg
     7921 gcccggtgct ggcggtgacc accttcaaaa cgatggaaga agcgctggag ctggcgaacg
     7981 atacgcaata tggcctgggc gcgggcgtct ggagccgcaa cggtaatctg gcctataaga
     8041 tggggcgctg catacaggct gggcgcgtgt ggaccaactg ttatcacgct tacccggcac
     8101 atgctgcgtt tggtggctac aaacaatcag gtatcggtcg cgaaacccac aagatgatgc
     8161 tggagcatta ccagcaaacc aagtgcctgc tggtgagcta ctcggataaa ccgttggggc
     8221 tgttctgata taagaagctg gtcgcattgg gtattcattg cctgatgcga cgcttacgcg
     8281 tcttatcatg cctacgggaa cctgaccgta ggccggataa ggcgtacacg ccgcatccgg
     8341 cataaacaaa gcgcacgttg ttacaattta cgttttaaac ctacgtgcgc aataacccag
     8401 actacgccaa cagaagtgcg tttctggggc gtgttttgtc aggagtttaa tctcgcgttt
     8461 cgatggtgag cgggcgaacc aggccgtaaa tcaccattgg tgatccgagg ccaatcagca
     8521 ataaacggta gataattgaa ggtttaatac gcttaatctg tactgtggat gttttactgg
     8581 agttattcat tagtttggcc aatagttcca ctatttcaat atgataaacc tcgtcctgtt
     8641 gaaaaaccag acagcacatt tcagaaagta tataaaaaac aatatgtcgt tatataaata
     8701 aggaaattgc actgcatcat ggcatttcat aatatgcgtt aattgttctt ttttatattc
     8761 tttgagggaa atatgttcct ggactatttt gcactgggag tgcttatttt tgtatttctg
     8821 gtgatattct atgggattat tattttacat gatattccct acctgatttc taaaaaacgt
     8881 aatcatcctc atgccgacgc cattcatgtt gctggttggg tgagtctttt tacgctgcat
     8941 gttatctggc catttttgtg gatttgggcc acgctttacc gcccggagcg gggatgggga
     9001 atgcaaatcc atgactcctc cgttgtgcaa ctacaacagc gtattgccgg gctggagaaa
     9061 cagctcgccg acatcaaatc ctcttctgcc gagtaatact tatggattta ctgattattt
     9121 tgacctatgt ggcttttgca tgggcaatgt ttaagatctt caaaattcct gtaaataaat
     9181 ggaccatttc cacagcggcc ctgggtggca tatttattgt cagtggttta attctgttaa
     9241 tgaactataa ccatccgtat accttcaagg cgcaaaaagc ggttatttct attcctgttg
     9301 tcccacaggt gacaggcgtg gtgatcgaag tgacggataa gaaaaatacg ctgattaaaa
     9361 aaggtgaggt gctatttcga ctggacccga cgcgttatca ggtgcgggtg gatcggctga
     9421 tggcggatat cgttaccgca gaacataaac agcgggcgtt gggcgcagag ttagatgaga
     9481 tggcggcgaa tactcagcag gcaaaggcca cgcgggataa attcgctaaa gagtatcagc
     9541 gttacgcacg cggcagccag gcgaaagtaa acccgttttc agaacgcgat attgatgtgg
     9601 cgcggcaaaa ttatctggcg caggaagcct ccgtaaagtc atcggcggcg gaacaaaaac
     9661 agatccagag ccagctggat agcctggtgt tgggtgaaca ttctcaaatc gccagcctga
     9721 aagcgcagct cgcggaagca aaatataacc ttgagcagac gatagtgcgt gcgccgagcg
     9781 atggttatgt gacccaggtg ctgattcgtc cgggtaccta tgccgcgtcg ctgccgctac
     9841 gtccggtgat ggtgtttata cccgatcaga aacgacaaat tgtggcgcag ttccgtcaga
     9901 actccttgct gcgactggcc cctggcgacg atgcggaagt ggtgtttaat gctctgccag
     9961 gtaaggtatt cagcggcaag ctggcagcca ttagtccagc tgttcccggc ggagcttatc
    10021 agtcgaccgg caccttacag acgttaaaca cagcgccggg ttcagatggc gttatcgcga
    10081 ccattgaact ggatgagcac actgatttga gcgctttacc agacggtatt tacgcccagg
    10141 tggcggtcta ctctgatcat ttcagccatg tctcggtgat gcgcaaagtg ctattacgca
    10201 tgaccagctg ggtgcattac ctttatctcg atcattaatc ttcgctttcc gccgcttgtt
    10261 caggccgcag ttgctgcgac tgagcaagct ggtggcgaat ttcacgcagc atttcatcca
    10321 gcacatactg gacccgccag ggcaggtatt cccgtttacg gaagatagcc accagatcca
    10381 gccaccattc atacgggtgc tgaaagcagg gcaccagcgt gccgtcttgc agatcgctct
    10441 gcacactttt gtctggcgca aacacaatcc ccaggtgatt tcgtgccagc tccagcgccg
    10501 attgcgtgtt atcgcaaacg taatttccct tcacgcgata atcccgcacc tctttactcc
    10561 cggcgacgtt gaagcgccag atattcgcgt cgtcgatcat catcgagtcg atcaaaatac
    10621 aggagtgatg ttcgagttcg tcaggacggc taataggatg tttctcaaga tagcgctggc
    10681 tggcataagc ggttacggca tattgtgtaa taaaactggc aaccagcgat tcatcttttg
    10741 gtggagcgta gctgattaaa acatcacaat catcaggaaa ttcgacgcct tcataaaagg
//
LOCUS       DAJTYG010000113        10706 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTYG010000113 DAJTYG010000000
VERSION     DAJTYG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.113"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            142..1839
                     /gene="maeA"
                     /locus_tag="OO568_003333"
     CDS             142..1839
                     /gene="maeA"
                     /locus_tag="OO568_003333"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6642566.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1973..2983
                     /gene="adhP"
                     /locus_tag="OO568_003334"
     CDS             1973..2983
                     /gene="adhP"
                     /locus_tag="OO568_003334"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR6642567.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVTDYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3129..3413
                     /locus_tag="OO568_003335"
     CDS             3129..3413
                     /locus_tag="OO568_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6642568.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIVPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3463..4116)
                     /gene="fdnI"
                     /locus_tag="OO568_003336"
     CDS             complement(3463..4116)
                     /gene="fdnI"
                     /locus_tag="OO568_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6642569.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4109..4993)
                     /gene="fdnH"
                     /locus_tag="OO568_003337"
     CDS             complement(4109..4993)
                     /gene="fdnH"
                     /locus_tag="OO568_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6642570.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYENAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5006..8053)
                     /gene="fdnG"
                     /locus_tag="OO568_003338"
     CDS             complement(5006..8053)
                     /gene="fdnG"
                     /locus_tag="OO568_003338"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7466..7468),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6642571.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8285..9166
                     /gene="yddG"
                     /locus_tag="OO568_003339"
     CDS             8285..9166
                     /gene="yddG"
                     /locus_tag="OO568_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6642572.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9425..10525
                     /gene="ompC"
                     /locus_tag="OO568_003340"
     CDS             9425..10525
                     /gene="ompC"
                     /locus_tag="OO568_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6642573.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         2624 a         2745 c         2654 g         2683 t
ORIGIN      
        1 agcattaccc aatgacgatt aaacaccatt gcctgcgcaa tggtgttttt atctgcttta
       61 tacttgggga cgacgccctg gcggtaaagc aaagacgata aaagcgtgcc agggatggat
      121 attcaaaaaa gagtgagtga catggaacca aaaacaaaaa aacagcgttc gctttatatc
      181 ccttacgctg gccctgtact gctggaattt ccgttgttga ataaaggcag tgccttcagc
      241 atggaagaac gccgtaactt caacctgctg gggttactgc cggaagtggt cgaaaccatc
      301 gaagaacaag cggaacgagc atggatccag tatcagggat tcaaaaccga aatcgacaaa
      361 cacatctacc tgcgtaacat ccaggacacc aacgaaaccc tcttctaccg tctggtaaac
      421 aatcatcttg atgagatgat gcctgttatt tataccccaa ccgtcggcgc agcctgtgag
      481 cgtttttctg agatctaccg ccgttcacgc ggcgtgttta tctcttacca gaaccgccac
      541 aatatggacg atattctgca aaacgtgccg aaccataata ttaaagtgat tgtggtgact
      601 gacggtgaac gtattctggg gcttggtgac cagggcatcg gcgggatggg cattccgatc
      661 ggtaaactgt cgctctatac cgcctgtggc ggcatcagcc cggcgtatac ccttccggtg
      721 gtgctggatg tcggaacgaa caaccaacag ctgcttaacg atccgctgta tatgggctgg
      781 cgtaatccgc gtatcactga cgatgaatac tatgaattcg ttgatgaatt tatccaggct
      841 gtgaaacaac gctggccgga cgtgctgttg cagtttgaag actttgctca aaaaaatgcg
      901 atgccgttac ttaaccgcta tcgcaatgaa atttgttctt ttaacgatga cattcagggc
      961 actgcggcgg taacagtcgg cacactgatc gcagccagcc gcgcggcagg tggtcagtta
     1021 agcgagaaaa aaatcgtctt ccttggcgca ggttcagcgg gatgcggcat tgccgaaatg
     1081 atcatcgccc agacccagcg cgaaggatta agcgaggaag cagcgcggca gaaagtcttt
     1141 atggtcgatc gctttggcct gctgacggac aagatgccga acctgctgcc tttccagacc
     1201 aaactggtgc agaagcgcga aaacctcagt gactgggata ccgacagcga tgtgttgtca
     1261 ctgctggatg tggtgcgcaa tgtaaaacca gatattctga ttggcgtctc aggacagacc
     1321 gggctgttta cggaagagat catccgtgag atgcataaac actgtccgcg tccgatcgtg
     1381 atgccgctgt ctaacccgac gtctcgcgtg gaagccacac cgcaggacat tatcgcctgg
     1441 accgaaggta acgcgctggt cgccactggc agcccgttta atcctgtggt atggaaagat
     1501 aaaatctacc ctatcgccca gtgtaacaac gcctttattt tcccgggcat cggcctgggt
     1561 gttattgctt ccggcgcgtc acgtatcacc gatgagatgc tgatgtcggc aagtgaaacg
     1621 cttgctcagt attcgccgct ggtcctgaac ggcgaaggtc tggtactacc ggaactgaaa
     1681 gatattcaga aagtctcccg cgcaattgcg tttgcggttg gcaaaatggc gcagcagcaa
     1741 ggcgtggcgg tcaaaacgtc tgctgaagct ttgcaacaag ccattgacga taatttctgg
     1801 caagccgaat accgcgacta ccgccgtacc tccatctaag cctgcacccg gtagtgaagg
     1861 ctaccgggct atttccctct ccctttttca gatctcatcc atactgggta gtggcgaata
     1921 aatctcattt gcctcacctg ctatgcagaa catcatccga aaaggaggaa ctatgaagac
     1981 tgcagttgtt acgaaggatc atcatgttga cgttacggat aaaacactgc gctcactgaa
     2041 acatggcgaa gccctgctga aaatggagtg ttgtggtgta tgtcataccg atcttcatgt
     2101 taagaatggc gattttggtg acaaaaccgg cgtaattctg ggccatgaag gcatcggtgt
     2161 ggtggcagaa gttggcccag gtgtcacctc attaaaacca ggcgatcgtg ccagcgtggc
     2221 gtggttttac gaaggatgcg gtcattgcga atactgtaac agtggtaacg aaacgctctg
     2281 ccgttcagtt aaaaatgccg gatacagcgt tgatggcggg atggcggaag agtgcatcgt
     2341 ggtcaccgat tacgcggtaa aagtgccaga tggtctggac tcggcggcgc ccagcagcat
     2401 tacctgtgcg ggggtcacca cctacaaagc cgttaaactg tcaaaaattc gtcccggtca
     2461 gtggattgct atctacggtc ttggcggtct gggtaacctc gccctgcaat acgcaaagaa
     2521 tgtctttaac gccaaagtga tcgccattga tgtcaatgat gagcagttaa aactggcaac
     2581 cgaaatgggt gcagatttag cgattaactc acgcaccgaa gacgccgcca aaattgtgca
     2641 ggagaaaacc ggtggcgctc acgctgcggt ggtaacagca gtagctaaag ctgcgtttaa
     2701 ctcggcagtt gatgctgtcc gtgcaggcgg tcgtgttgtg gctgtcggtc tgccgccgga
     2761 gtctatgagc ctggatatcc cacgtcttgt gctggatggc attgaagtgg tcggttcgct
     2821 ggtcggcacg cgccaggatc taaccgaagc cttccagttt gccgccgaag gcaaagtggt
     2881 gccgaaagtc gccctgcgtc cgttagcgga catcaacacc atctttaccg agatggaaga
     2941 aggcaaaatc cgtggccgta tggtgattga tttccgtcac taagaggtct ttgctgcgac
     3001 tcccatgttc gggtcgcagc atcgcacact ctccaacatg aaatggctaa atggattatt
     3061 gttgcgtagg aaggaacaat aatgatagtg ttcccagaga agtaacgcta ctgagagtaa
     3121 agacactaat gaaaatggcc aatcatcccc gcccggggga cattattcag gaatcactgg
     3181 acgaacttaa tgtcagcctg cgcgagtttg ccagagcaat ggaaattgtg ccctcaacgg
     3241 caagccgatt gctgaccgga aaagcagctt tgacgccaga aatggcaatt aaactttccg
     3301 tggtgatcgg cagttcgccg caaatgtggc tgaatctgca aaacgcctgg agtctggcag
     3361 aggcagaaaa aacggtggtt gtgtcgcgat tacgccgtct ggttacgcaa taaaaaatgg
     3421 caccttataa agcgccattt tcagcttaaa gttcaaaaga gattatatcc cttcttcact
     3481 ctcttttttc gcttctgcct tctcgatttc acgataccag cgcggatggt gtttcttcgc
     3541 ccagcgacgg ctcaccttcc cttcgatcat ccctttaatc gaccctttca cccagaatgc
     3601 catatacata tggatcagga tggcgtggat caggatgata cccgcagccg cgtggatcag
     3661 caggctgtag cgaacaacct gcatcgggaa gtactgcgca aagtacggac gccagataat
     3721 caccccggtc accagcagca cgaaaatcat gctcatgatc gaccagaaca tcatcttttg
     3781 cccggcgttg tacttaccaa catccgccac tttgtgctca ttgcctttta atacttcgac
     3841 aatgttcaac agccacggaa tatctttctt atccgggatg ttgtgatgca caaaacgcac
     3901 aaacataaac atcagtgcga cgaaaatcgc aatgccgaag aacgggtgca aaatgcgtcc
     3961 catctgcggc gtaccgaagg tttgcgtcag ccattgcagc gtcgggaaga agaacgaaat
     4021 cccggacagc gccaccagga agaagcaaat caccacggtc cagtgacagg cgcgatcaat
     4081 aaatttggtg cgcacaatca ttttcgactt actcatgatg atcctcctcg tcatcgtcca
     4141 cttccttatt cgggccaata ccgatgtagt ggaaaatcaa cccggcaaaa gtggcaataa
     4201 agccagccgc tgccagcggt ttcaacgcgc ctttccacag gcttaccgag gtgtcgatct
     4261 tcggatcttt cggcagaccg tgatacagct ccggctgatc ggcgtgatgc agcacgtaca
     4321 taacgtgcgt accaccgacc ccttccgggt tgtagacgcc agcattttcg taaccacgcg
     4381 ctttcagttt cgccacgcgc tgttccgcca gctccagcat ctccttcttg gtgccgaagt
     4441 ggatagcccc ggtcggacag gttttcacac aagccggttc ctggccgacg ctgacgcgat
     4501 cgacgcagag cgtgcattta tatacccggt tatcctcttt gttgaggcgc ggaatattaa
     4561 acggacaccc ggcaatgcag taaccacagc cgatgcagtt ttccgactgg aaatcgacaa
     4621 tcccgttagc gtactgaatg attgcaccag cagacgggca cgccttcagg cagcccggat
     4681 cttcacagtg catacagccg tctttacgga tcagccactc cagcttgcca ttctgttcgg
     4741 tttcgctaaa gcgcatcacc gtccaggact tggcgctcag atcggcgggg ttatcgtaaa
     4801 caccgacgca gtgccccact tcatcacgga tgtcgttcca ctccgaacac gccacctgac
     4861 aggctttaca gccgatacag gtggaaacgt cgataagttt cgcgacttct gctttgtaat
     4921 cacgcacctg agaaggcggc gtgatggagt tagttgcgga ccttttgata atatcctgcg
     4981 tttccatagc catctgttcg cccccttacg ccttctcgat gttgactaag aacgctttat
     5041 attccggcgt ttgcgagttt gcatcaccga cattcggcgt cagagtgtta gcgatataac
     5101 ctttacgcgc gacaccctca aagccccagt ggattggaat acccaccgtt tcaacctgct
     5161 gaccgtttac attcagcggt ttcagacggc gcgttaccac tgccaccgcg cggataaagc
     5221 ctcgcttgct ggagacagtg acacgatcgc cattattaat gcctttcgcc gccgcctgcg
     5281 tttcgctgat ttccacaaac tgttccggct gagcaattgc gttgagcaac gcgtgcttgg
     5341 tccaggtgtg gaagtgctcg gtcagacgat aggtcgtacc cacatacggg aactgctctt
     5401 ttttacccat ccgcagcgcg tcttgttcat acagacgaac aaccgggtta gacaccacgt
     5461 tcggatgcag cgggttagtg cccagcggcg tttcaatcgg ctcgtagtgt tccgggaacg
     5521 gaccttccgc cattttgttg atggcaaaca ggcgtcccat cccttccggc tgcataataa
     5581 acggcccggt tggcgtaccc ggtgcggcat tgccgaagtc cggaatatcg ttacccgtcc
     5641 acttgctgcc gttccactgg atcagcatcc gtttcggatc ccacggttta ccgttgatat
     5701 ccgccgaagc acggttgtag agtacgcgac ggttgagcgg ccacgcccag gcccatccca
     5761 gcgtattccc cagaccggac gggtcggagt tatcgcggtt tgccatctgg ttaccctgct
     5821 ctgtccagct accggtgtag atccagcaag aagatgcggt tgtaccgtca tcacgcagat
     5881 gcgcaaagct actcagcaac tgacctttct tcgcaatcag cacgccatta gcatcataga
     5941 gatcttccaa cgcatagccg ttgttctctt tagctacttc gtcagattgc ggttcgtgcg
     6001 gctgcttgta gttccagctc atcttcatca gcggttctac gcctttacca ccttcggcct
     6061 ggtacaactc gcgcagatga tggtagatac ccgccagaat ttcgccgtcg ttacgcgctt
     6121 cgcccggcgc gtcctgacct ttccagtgcc actgcaacca gcgaccggag ttagcgatag
     6181 aaccatcctc ttcagcaaag caggtcgaag gcagacggaa tacttcagtc tgaatagacg
     6241 ccggatcgac atcgttcgac tcgccgtggt tctgccagaa ggtagaggtt tcagtcacca
     6301 gcggatcgat aaccaccatg tacttcagtt tgctcaggca gctcaccact ttgtttttat
     6361 ccgggaagga cgcaaccggg ttaaagccct ggcagaaata accggtgact ttgccttcat
     6421 ccatcatgtt gaaatacttg atgacgtcgt aggtctggtc ccacttcggc agccagtcat
     6481 agccccagtt gttctctttc tgcgcggcat cgccatagaa agatttcatc aggctaacga
     6541 agaacttcgg atagttgctc cagtagttca cctgatcagc cagcgtcgct ttcggcgtgt
     6601 tcgcttccag atacgactgc aaatcaacct gtttttctga cggcagcgtc agataacctg
     6661 gcaggctggt agagagcaga cctaagtcag tcagcccctg aatgttggag tgaccacgca
     6721 atgcgttcac gccgccaccg gccataccca tgttaccgag cagcaactgg atcatcgcca
     6781 tagtacggat gttctgcgca cccacggtgt gctgcgtcca gcccagcgcg tacaggaagg
     6841 tggttgtgcg atccggtgcg ctggtggagg ccagcacttc acacactttc aggaagtcgg
     6901 cttttggcgt accacagatg ttttcaacaa cgtccggcgt gtagcgggaa acgtgcgctt
     6961 tcagcaggtt ccacacacag cgcggatgag tcagtgtttc atcgcgtttc gcatagccgt
     7021 tttcatcgaa ctgatagttc caggacgatt tgtcgtactg gcgtttttca gcgtcataac
     7081 cgctgaacag gccatcttcg aaagcaaaat catcacgcac cagtaggctg gcgttggtgt
     7141 aatgtttaac gtattcggcg ttgattttgt tgttttcgat caggtagcgc aaaacgccag
     7201 acaggaacgt aatgtccgta ccggaacgaa taggtgcgta aatatccgcc acagaagcgg
     7261 tacgcgtaaa acggggatcg acaacgatca aggttgcatc gttgttgttt ttcgcttcca
     7321 tcgcccagcg gaaaccgacg ggatgcgctt cagcagcgtt accgcccatt accattacga
     7381 cgttagcgtt tttgatatcc acccagtggt tggtcatagc accgcgacca aatgttggag
     7441 caagacttgc taccgttggt ccgtgtcaga cgcgcgcctg gttgtctacc gccagcatcc
     7501 cgagggagcg ggcaaatttc tgggtcagca tcccggtttc gttgctggca ccggaggcac
     7561 acagcatacc ggtagaaagc caacggttta ccgttacgcc ctgctcgttc ttttcaataa
     7621 agttagcgtc acggtcagct ttcatcagct tcgcaatacg ggagaatgct tcttcccagc
     7681 taatgcgctg ccatttgtca gaacctggcg cacgatattc cgggtagcgc agacggtttt
     7741 cactgttgac gtaatccagc aaaccggccc ctttcgggca cagcgcacca cggcttaccg
     7801 gatgatccgg gtcaccttca atgtgataaa tcgcttctct ggcgtttttt gcgccatcac
     7861 ccaggctata catcaatagc ccgcaaccta cggaacagta tgtgcaggtg ttacggatct
     7921 ctttagcgcg taataatttg tagtttcgcg cctgagccag tgcttgcttc ggggcaaagc
     7981 ccaatgccgc taccgttgtt ccagccatac ctcccgcgca gattttaaaa aattgtctgc
     8041 gactgacgtc cattgctttc ctcttttttc aggggtatta ctgcgcgagg aaactaacag
     8101 taaaattctg aatctttttg cgcgaaatca aaaagaacac gtgtttgcca caaaataatc
     8161 cgcctgcgca aattgaccta cctcaatagc ggtagaaaaa cgcaccactg cctgacaggc
     8221 cagttaaaaa aatgctataa aattcagctt aatttttaac ggcaagagag acaaaacagc
     8281 gagcatgaca cgacaaaaag caacgctcat agggctaata gcgatcgtcc tgtggagcac
     8341 gatggtagga ttgattcgcg gtgtcagcga ggggctcggc ccggtcggcg gcgcagctgc
     8401 tatctattca ttaagcgggc tgctgttaat cttcacggtt ggatttccgc gtattcggca
     8461 gattccgaaa ggctatttac tcgccgggag tctgttattc gtcagctatg aaatctgtct
     8521 ggcgctttcc ttagggtatg cggcgacccg tcatcaggcg attgaagtgg gtatggtgaa
     8581 ctatctgtgg cccagcctga caattctctt tgccattctg tttaatggtc agaaaaccaa
     8641 ctggctgatt gtaccaggat tattattagc cctggtcggc gtctgctggg tgttaggcgg
     8701 tgagaatggg ttacattatg atgaaatcat caataatatc accaccagcc cattgagtta
     8761 tttcctggcg tttattggtg cgtttatctg ggcagcctat tgcacagtaa cgaataaata
     8821 cgcacgcgga tttaacggaa ttaccgtttt tgtcctgcta acgggagcat gtctgtggat
     8881 ttactatttt ctcacgccac aaccagaaat ggtatttagc acgcccgtca tgattaaact
     8941 catctctgcg gcatttacct taggatttgc ttatgctgca tggaatgtcg gtatattgca
     9001 tggcaatgtc accataatgg cggtaggctc gtattttacg cctgtacttt cctcagcgct
     9061 tgcagccgtg ctgctcagcg ccccgctgtc gttctcgttc tggcaaggcg cgctaatggt
     9121 ctgcggcggt tccctgctct gctggctggc gacacgtcgt ggttaaaata attgccgggt
     9181 ctacggcccg gcagttaatc agataaattc cgacaatatt aaaaaataaa tttcaaatac
     9241 cagtaattca ccttatatct catttcgact tcgatcacat atcgataaat aatattattt
     9301 catctatttt tctcattgtt tttaatcacc attctatttt aaaaacagta tgtcatttat
     9361 aaatgacgcc gtttaaaatt cgtttagaaa atacgtttaa cgataattaa aaggattctg
     9421 ttaaatgaaa ttaaaaatag ttgcggtggt tgtaactggt ttgttagctg cgaacgtagc
     9481 acacgctgcc gaagtctata acaaggatgg taataaactc gacctttatg gcaaggttac
     9541 cgctctacgt tattttactg atgataagcg tgacgatggt gataaaactt atgcccgtct
     9601 cggctttaaa ggagaaacgc aaatcaatga tcaaatgatt ggttttggtc actgggaata
     9661 tgattttaaa ggctataacg atgaagccaa cggctcgcgc ggcaacaaga cccgtctggc
     9721 ctatgctggt ttaaaaatta gtgaatttgg ctctctggac tatggtcgta actacggtgt
     9781 cggctacgac attggttcat ggaccgatat gttgccagaa tttggtggcg atacctggag
     9841 tcagaaagat gtcttcatga cataccgtac caccggtcta gcaacctatc gcaactacga
     9901 tttctttggc ttaattgaag ggctgaactt tgccgcgcaa tatcaaggca aaaatgaacg
     9961 tactgacaac agtcatcttt atggtgctga ctacacgcgt gccaacggtg acggtttcgg
    10021 tatctcctca acttatgttt atgatggctt tggtatcggt gcggtatata ccaaatccga
    10081 tcggacaaat gcgcaggaaa gagccgctgc taatcctctc aatgcctccg gtaagaatgc
    10141 agaactatgg gccacaggta taaagtatga tgccaacaac atctactttg cagctaatta
    10201 cgctgaaaca ttaaacatga ccacctatgg ggatggttat atctctaaca aagcacaaag
    10261 ttttgaagtg gtggcgcaat atcaattcga cttcggcttg cgcccatcac tcgcttacct
    10321 gaaatcgaaa ggcagagatc tgggccgcta cgccgaccag gacatgattg agtatatcga
    10381 cgttggtgcg acgtatttct tcaacaaaaa tatgtcgacc tatgttgatt ataaaatcaa
    10441 cctgattgat gaaagcgact ttacccgtgc cgtagatatt cgcaccgata acatcgtcgc
    10501 tacgggcatt acctatcagt tctaacgtcc tctcctgacc agcccttctc tgggctggtt
    10561 tttgatttaa gtgctgctgt atgactcctt aagtaaaaat gagcaatttt tgatatttct
    10621 ggtcattcac ctcaattaat attattccat atcctccggg gacatcttac tataatattt
    10681 attgatgagt gattatgacc aatatt
//
LOCUS       DAJTYG010000114        10385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTYG010000114 DAJTYG010000000
VERSION     DAJTYG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.114"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            338..700
                     /locus_tag="OO568_003341"
     CDS             338..700
                     /locus_tag="OO568_003341"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HCR6642574.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            697..1614
                     /locus_tag="OO568_003342"
     CDS             697..1614
                     /locus_tag="OO568_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6642575.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            1627..2892
                     /locus_tag="OO568_003343"
     CDS             1627..2892
                     /locus_tag="OO568_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HCR6642576.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(2959..3582)
                     /locus_tag="OO568_003344"
     CDS             complement(2959..3582)
                     /locus_tag="OO568_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6642577.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSERY
                     THTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGNNCELIDIGCTFMEIT
                     SKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVTFSHPLAAATNGYVVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(4333..4671)
                     /locus_tag="OO568_003345"
                     /pseudo
     CDS             complement(4333..4671)
                     /locus_tag="OO568_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
     gene            4793..5053
                     /locus_tag="OO568_003346"
     CDS             4793..5053
                     /locus_tag="OO568_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCR6642578.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQCAKMNNSSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(5071..7806)
                     /locus_tag="OO568_003347"
                     /pseudo
     CDS             complement(5071..7806)
                     /locus_tag="OO568_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(7807..9138)
                     /locus_tag="OO568_003348"
     CDS             complement(7807..9138)
                     /locus_tag="OO568_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6642579.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPNVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPQDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            complement(9148..9840)
                     /locus_tag="OO568_003349"
     CDS             complement(9148..9840)
                     /locus_tag="OO568_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6642580.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            complement(9843..10298)
                     /locus_tag="OO568_003350"
     CDS             complement(9843..10298)
                     /locus_tag="OO568_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR6642581.1"
                     /translation="MIQFKPTRNIDLIEAVGNHTDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            complement(10298..>10385)
                     /locus_tag="OO568_003351"
     CDS             complement(10298..>10385)
                     /locus_tag="OO568_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642582.1"
                     /translation="LASNGSTLTIQSNGAAFTATTIKIIAEQ"
BASE COUNT         2644 a         2444 c         2262 g         3035 t
ORIGIN      
        1 tccgctattg ccagtacacc tcaaacctac gaccttcgca ttacgaatct gtagcaccaa
       61 tcataactat ctgttttagc aagcattaac cgcattcact aagatatagt tgatggcaca
      121 aacagaaagt tgatgaatga tgttgccata tatatgtcac aaatacggca caacgatctt
      181 caaacatata gccacacatt cacagaagag cacaaagcct tgcaatccag tgcaaagatt
      241 tgtgtgccag agtttttctc atcaactacc gcaagtatcg atcgattgag acttggatga
      301 tagacttcat gcctttgatt attagctgat agaagaaatg ttaaagctat ttgcaaagta
      361 cacatctata ggcgtgctga acacacttat acactgggtg gtttttggtg tatgtatcta
      421 tgccgcgcat accaatcagg ctatggcaaa cttcgcaggt ttcgttgtgg ctgtgagttt
      481 tagcttcttt gcgaatgcaa aattcacatt caaagcatca actacaacga tgcgctacat
      541 gttatatgtc gggttcatgg gaacactgag tgctactgtt ggatgggttg ctgatagatg
      601 ttcacttcct ccaattgtca ctcttgttac cttctccgcc atcagcttgg tgtgcggttt
      661 cgtctattca aagttcattg tctttaggga tgcgaaatga agatatctct tgtcgttcct
      721 gtcttcaatg aagaagaagc gataccaatt ttttataaaa cggtacgtga attcgaagag
      781 ttgaagccat atgaagtaga aattgttttc ataaatgacg gaagcaaaga cgctacggag
      841 tcaatcatta acgctctggc tgtttctgat ccgctagttg ttccgctgtc atttacacgc
      901 aactttggta aagaaccagc attgtttgca gggttagacc atacaaccgg cgatgccgtg
      961 attccaattg atgttgacct gcaagacccg attgaggtta ttcctcatct tattgaaaag
     1021 tggcaggcag gtgctgacat ggtgcttgct aaacgttcag accgctcaac tgatggacga
     1081 ctgaaacgta agacagctga gtggttctat aaattacaca acaaaataag caccccaaaa
     1141 atagaggaaa atgtcggaga ttttcgactc atgtctcgtg aggttgtgga gaacattaaa
     1201 ctattgcctg agcgcaatct tttcatgaaa ggcatactga gctgggttgg tggtcagaca
     1261 gatgtcgttg aatatgtacg cacagagcgt gttgctggca tctcaaaatt taatggctgg
     1321 aaattatgga atctggcact ggaaggtatc acaagttttt caacctttcc tcttcgcgta
     1381 tggacttata taggcttgtt tgttgcaagc atttcatttt tatatggtgc atggatgatt
     1441 atagacacca ttgtctttgg taacccagta cgcgggtatc cctccatgct tgtatcaata
     1501 cttttcctgg gtggagtgca actgatcggg attggtgttc tcggtgaata tataggtaga
     1561 atttatttag aaacaaaatc aagaccacgg tacttaatta aaagcagaaa ataacaggat
     1621 gatcttatga agatattcat aatgcgcaat tggcacaaga tatcaatttt tatacttgca
     1681 tttactttga tttggcttag gcgcattgac attttgacaa atgcccagtt ttgggctgag
     1741 gatgcagttt tttggtataa agatgcatac gaacaaggtt ttttcagttc actcataacc
     1801 ccaagaaatg gttattttca aacagtatca acactcattg ttggtgcgac gacatttatt
     1861 aatccaattt acgcaccatt attatcaaac ttttttggaa taataattag agctataatt
     1921 atatggttct tatttaccga tagatttaaa ttcctaagca ccacatcaaa aatcttcatc
     1981 tctgtatatt taatttgcat gcccgggctt gatgaagtcc aagccaatat aacaaatgct
     2041 cactggtatt tatcattgta tgttgcaatg ataataatat ctgaagaatc caagtcaaaa
     2101 ttatggaaag cacatgattt attttttata gtattatctg gattgagcgg tccatttatt
     2161 atattcataa ttgcatcatc tgtttttaaa tatttacact tatcaaaagg agtaataagc
     2221 ataagaggtt tatacttatt ttatactcga ctgccatatt tggcaatgat cttgtgtggg
     2281 ctgatacagg caacatcaat aattcttaca tttaatggaa caagatcaca tgctccatta
     2341 ggattcagtt ttgatgttat gtcatctata atatcatcta atgtatttct tttttcattt
     2401 gtcccttgga atatcgctaa tgctggatgg gataatcacc ttttatcgta cacactatca
     2461 actttagtaa ttacattatg tgttttcatt tatattaaag gaaattggca aatgaaagtg
     2521 tttgccaccc ttccaatact tattgtcgca ttttctatgg caaaacctca actggcagat
     2581 tctgttcctc aactgccaac acttgcaacc ggaaacgggt caaggtattt tgtaaatatt
     2641 catattgcaa ttttttcatt aatatgcgtt tgtttgtttc aatgcataaa gaataaaacc
     2701 ttaaaaatat tctttaaaat ctatgtttca gtcatcctta ttgcaatgat aaaattaaac
     2761 ttttttataa cccctcttcc tgacatgaac tggtctcaag gagcagaatt aataaataaa
     2821 gcaaaacatg gagaagccgt ctcatttaca gtgctccctc catggttaac tcttgattta
     2881 ataaaaaaat aaaacttcac attaataaat aacaatgaat ccgcctccct acgtattccc
     2941 gtagggaggc tgtcgtgttt atagtagcct ggtaatgcta aatgcaaacg aactggagat
     3001 tcctgctccg ctaagcgtat tgcgcagggt caccctaaat gtatctacac caatataagt
     3061 aacatcacaa ctaactgaat caggcgcaga tataggtgtt atctgaacca cataaccatt
     3121 agtagctgct gcgagcggat gagaaaacgt tacatcatac acaccagtag catgcgcgtt
     3181 aacggatgaa acacccggcg caatgtctac actaccattg gcgttaattc tccctgagcg
     3241 cgtaactgac tctgcagaaa ccgatgtggc cttactggtt atttccataa aggtgcaacc
     3301 aatatcgatc aattcgcaat tgttccctgt tatccagtat ggtcgatctg cacttggctg
     3361 ataatcccct gcgctctgga acgttgtgcc attgagtaag actataactt ttcccccacc
     3421 tgagcttgtt acttgtatat tcgtgtgcgt gtagcgttca ctgctcacgc gatgaaaatt
     3481 tccaccgttt ataactaccg ttacaggtcg cgtcccagta ttatctatag caaggtctgc
     3541 ccccccggca ttagcctcga agtatgggtt gtttaacacc aaagcacacg aaccgttaag
     3601 gccatttatg gctaattgcg caccacctgt accggaatcc ccctgagtac catttccttc
     3661 acaagtcagg ctgttgatag tagttcctgc cccccatcta tcaccaagta taccccatcg
     3721 tgtattctgg cttgcagtta atcggttaac tgtaatggca ttaagctctg acccttcact
     3781 ggagttaagt gcgcgccagc caatacggtt accctgtaag tacacattat ctaactctga
     3841 cgttaacacc gagtctgtac gcaacccttc gccggtagtg tgcccataag acacaatgtt
     3901 ctctaattta gtgtatgctt tattggttac gagaagaccg taagacgatc ccgaaccttt
     3961 aagtgtaatg tcccccagat aatcataggc gtgtacacgt tgggttgccc cgactgtatc
     4021 accgagcatc tttaagttga aatccgaggc atggcagtta aatatcgttt ctgccatatt
     4081 ttctccggaa taatgagcac ggactcctgt cgaaccaata aatttagcgt catagttgcc
     4141 gtaatcaaga acgagtggtg acgcgatatc gtacacccct tgctttattt ttaatgttcc
     4201 accgaaaggc cacgatgtaa gcgggtcttg atatatttca ttttttattg caaatagctc
     4261 ctcttggaga tttttgccgc tattagtgcc aatgtgagca gctccttcat ctgattgaag
     4321 taactgcttt aactgatcag gatcgtactt cagcacattc tgaaaataga actgctgcga
     4381 accatacgca tcatatacag ccattgaatg gccttgctca gttacaaatt tggcaatctg
     4441 cccgttgtac acaggatatc cggcagcatt gataacaatt ggctgagaaa cagggacgtg
     4501 agatccgtct tcattctcta cataaacctg aatctggttt tcaggattta ccgggtcagt
     4561 gtcaattttt ccgatataaa ttttgccatt ggctacggct ttaaaagaac gcgccatagt
     4621 gaagagttgc gaaggcatgc tgaccacaac atttgcggcg atatctgaca tttcattgct
     4681 ccagacgaat gatatgatgc aatcatgatg tgattgcata ccgaaatggc actattgaat
     4741 atttatccag taggttacga tgccattcca cccaactggt gaggcatcaa ggatgtacag
     4801 caaatacgac gaggcacagt ttcacttgag acttccgcat gaactccacg cgaaaattaa
     4861 acagtgtgcg aagatgaata acagttcgct gaactcagag ataattgcag cgattgaaga
     4921 atcattggct aaacaaagct ctgcatcagt ttacattgac gatgcagagc gtatggcaga
     4981 acaacaatct gatatggtta agaaaattgt ctttgatacg ctcaaggagc tatataaaaa
     5041 agacagcaac taaccatccg tggctttccg ttacttctgg ttatcatcac tgttcatcca
     5101 gtacatgatg cctagccttg atatggtttt cttatctgat tctggaagtg agttgtaaaa
     5161 cgagcgccaa gcagaagagc gtcttacagt tgtatcaacg atgctttctg gtgcttgttt
     5221 tactgccagc cttgctgctt tctggaattc tggtgagcta attagctttt ctgcagattc
     5281 aattgattca gcgccgccca tcgccctgat tttgctggcg acttttgccc caatttctga
     5341 cccagcaaca gcacctactg cactgaacgg tcctgacaca gttgaaccaa ccgcagtgcc
     5401 aatgcgttgg gcatggttag caacaaattc atttgccgca gtgacgcgat taaaccgttt
     5461 gacgaactcg tttagtcttc ctgtagttat ctcgtaagat tttgcatctt tgatagcctt
     5521 tgcgacctta tatacatcgt tcagacctga catagtctct tttggtaaat gtctggcaag
     5581 attgcgcatc tgcccgtttg ctaacatgtt ttgataccag tcagcaaacc ctgcaggatt
     5641 aaaatcagcg ccacgttttc cgttcgaaag catatcccga agacctgttc ccagtagctc
     5701 ggttcttagc ttcctggacg gcgtgttttg catcaattca cgaaatcctt tggcatcgcc
     5761 tttcgacatt gcctgtagtg ctgtagttgc tttgcgagaa acatcaccgt tcagcgttct
     5821 gccagttaga ttaatcattt gctcttccat gctttttcgc atctgaacaa gcctctgagc
     5881 aacttcaaaa tcacgtaatg catttgtctc tgacagcgcc gccttttgat catcggcgag
     5941 cgaaccgtaa agcctcgata aagcagcgcg atcagcatct ttatatggtc cagaattctt
     6001 atgaagtgca tcaccaacaa gtcgtcgttg cttattgaga tttgcataag ttaatgcgcc
     6061 gttcttgcct ggattaactg cccgaaagac tctcttttca atagggtcaa ggtttttcat
     6121 cccgccaaga tcttctgcca cctgtttgag cattgacctt gttgatggcg catcaatttt
     6181 tgcacctgca ggcatcgcct tttcaacatt gcgataaagc tggtcgcttc gtttcatcag
     6241 cgcagacatt cttccattga ccgtatcaat aaacttgtcg ctcataccaa gagcatcttt
     6301 tgcaccggaa acgcgatcaa ttatctcacc cgcgccctgt gctagctgcc tgattgcttc
     6361 attttcctgc acctgtagag cagaacccgc acgggacttg attgcttgct caactgcctt
     6421 gtattgctcg ttcccggaaa agtgtgaagg gagtaatgaa tcaacattaa gcctttcagc
     6481 agactccaga acttcagcct gaggattgat atctagttca tcaagcgatg aagcgagatt
     6541 tggccttttt tgtgctgcta ctgtgcgaac gacttcctct gggttcttcg tcgcaggcgt
     6601 cattggtaca ccttgtgcta ctcttgcctc tgcggcatga accgcaggat ttgttgctgc
     6661 agcggcatca ccggagaatg gtgatgtttc tgttggcgca gtggtcgctc tctgtgcaga
     6721 tgttttattg ccactaatta tatttgatgc accacgaaca ccacgggtaa cgccatttat
     6781 caggccatgc gtagcaatgc taagtccggc attaatagcg gcattttcgg caaaatcacc
     6841 ccgttgattc gccgcgtcag cgagagaacc aatgaccatg tttccagcta cacctgctcc
     6901 aggaacaaga taaccgccta ttgactctcc agcttgcgcg taggggtctg tcggtctgtc
     6961 tactggacga taaacatcat ccaatacttt tggcccccca agcccctgac tgattgcatt
     7021 aatcagactt gcgccaccct gcaatacgtc aaatggtatg tttaccagac cacgaccagc
     7081 ctgttctgca atttaccctg cactttgacc accagtgagc caatcgccag cttgttgcat
     7141 caatgatggt tcttcccgtg ttggtgcatt attggcctga ttaactgttt gttgctgaac
     7201 aggtttcggt gcgacagaac ttacgggttg aggtggctgc tgactggctg cctgctgctc
     7261 aatctgagca aatggattat ttgggtctga ctgcacacct gatgccgata cttgttcgga
     7321 tgactgtact tccagttgtg caaacgggtt gttagggtct tgctgaggat gtacctttgc
     7381 agaggtggcg cgctgttcga ctgttgagtc tgtcaccggg tcaccagccc attgagcaaa
     7441 gcgatcatca acgtaaccgc ggccttcagg tcctggcgta tattcaccac gctttgcctt
     7501 cataacgttg ccgggaccgt cgtgataagc ctgaagagcg tcacgccagt taccaaactg
     7561 ctggtacatc tttgccagat agcgcgcgcc agcgtcagcc tgatattcgg ggttttgcat
     7621 ttgctcatcg gtataaccca tatcacgcca tgtcccaggc atgacctgag tcaatcctac
     7681 agccccggcg gagcttactg cggcagggtt gtaagaagac tccttggcac ccagtgcagt
     7741 catcaaccct tctggcacac cgtaacgtgc gccagcctgc tctaacaaat cacggtaatt
     7801 agccatttac tgccccaaag atggaagata tccgtagcga ttaatgaagt cgattgacag
     7861 ctcggggtgc tgcttcaggt aatctataga agcctgaggc gcttccactc gcttgatacc
     7921 gttttgctga acgtacttac caaccgcctc attacgcttc tggttgagcg tgttcaggat
     7981 gacgccagcg ttgcgacgaa aggactcctc gctctgcgag ttctgcagcg aaccaacagc
     8041 ctggtcgagc tttttgccct cggcatcaga aagtgcgccc atgcctcgca tggcctgaac
     8101 cgctgtcagg tatgcttggg atttaaaggt atcaagtcgt gcctgagtgt ctgcagcctg
     8161 tgagcctgga acgttgggga ttactccacg taagcctgta atgctcttaa gtgaaggaga
     8221 actaacgata tcgttcagag tgaacatgct ggttgtgagg gtgttgatgc cgtctttgta
     8281 gccatcattt agcgcttgct gcttctgctg caactgctgg ttgttggctg ctatgcggct
     8341 ctgtatttcc tggcgcttca ggtcgttagt ttctgctgat agcatccggt caaggcgctt
     8401 attttcgttg ttaatgcggt ttgtttctgc gtccagatta atgcgctgct gacctaaatt
     8461 cgcctggata tcttgcccgc gcattgtgat tgcctgattc cgagcggcgg tttgcgaatc
     8521 caggtcctga ccgcgcatgg taacctggcg accctgcatt ttatcctgta ggtcaaagta
     8581 tttttcgggt ccgagactgt tcatccccag gtgatcgaca aattcgccga actgccgcgg
     8641 gttctgttgg tacatctgag cgacgtcctg aggattaacg ccaacacgag ctaactcacc
     8701 ggcgttgttt tgcagccatg attgcattgc ttctggagac gatgacgcaa ggcgtgcgcc
     8761 agccgctaag gtgccgatag aattacgctg gtcttcatca atgaatccca tgcctttacg
     8821 aacggattca atctggtctg gatattgagt agccaactga cgcaaagcac cgcgatcacc
     8881 agacgcataa gcattagcgt acgcctgctg aaattctttc tgccgctgag cctgcttttc
     8941 ctgctgaaac acccccgcaa tacctgaaag gccttgcaaa gcagtcagcc caacattgtt
     9001 agcgcctgaa cgctcaatat cattgttctg cctgataagc tgaagcgtat tgccgatgtc
     9061 atttacgttc ggagcgtttg agttgacgcc gccgatacca gccaataatc cgccatttga
     9121 tccttgccaa gtagccatga ttacccctta aaacaacgag ccaagcaatc cgataccagc
     9181 accaatgcca gcgccccaag gtgttgatgt tcccaaaagg ctggcaagac ctgcaccggc
     9241 aatcgcacca gacgttccgc cactaattgc tgtctgaaga cttgatggtt tgttggcatt
     9301 agcagcggca agagctgcgc tttgctgtgc aatgctgctc atgttgttgg cgtatgtctg
     9361 cccggcgttt gcctgacctt gcagcgcacc aagcccaacg tttgccagat tgttgtaatt
     9421 gctcatctgg tttgataacc aagactgacc gagtgtcggc gcgatcgtag ccagttgatt
     9481 gcttgtggct gtcgaaccaa gtccacccgt cgcctccgca gcagcaagac tctggtaacg
     9541 agcctgacct gcaaggtctt tatactgctg agagttgtaa tactgattaa gtgcctgccc
     9601 ctgaccttct aaactggaaa ggttctgaag ctggttaaca tactgctccg caagaggcgt
     9661 gaacggagca aggtttttca tgatcgtctg ccactgctga ttttgcaggt ctgcggcata
     9721 cttctgagct tctgctgcat actttgcgct tttatcagag ctgccacctt tcccgccttt
     9781 ttcagggcaa taaggttcct cgccgcgcag ttttctgccc agcttaaatg catataacat
     9841 ggctatctcc cgtgattcag gaagtcgatt agttcttcgc gtgtggcgct gtaaaacgtc
     9901 acgtcatcca cgcctttgaa gtatttcttg atggttccta cacgcttaag gccaatcatt
     9961 gcgcagtaca tctgaccgtg gcggaatttg cgcgcagcga acgatgtgac gcactgaacg
    10021 gtggtgttag tcagaatgta tcgccagaac gccagcccga tttccttgct gaatccacga
    10081 atctctggca ggtacatggc gtggcaatca aaggttagcg gctgaatctc ctgatagtaa
    10141 acaattccgc cgaactgccc gtgcacgtta acctcaaagt aacggcattc aggcttgtag
    10201 tcgtatccat cgccgttgtt gctcccggcg ataatgtcag tgtgatttcc tactgcttcg
    10261 atcaggtcga tgtttcgcgt tggtttgaac tgaatcatca ctgctccgcg attatcttga
    10321 tggttgtggc agtaaacgcc gcaccatttg actgaatagt taacgtactg ccatttgagg
    10381 caaga
//
LOCUS       DAJTYG010000115        10270 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTYG010000115 DAJTYG010000000
VERSION     DAJTYG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.115"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(329..673)
                     /locus_tag="OO568_003352"
     CDS             complement(329..673)
                     /locus_tag="OO568_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6642583.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            805..2715
                     /locus_tag="OO568_003353"
     CDS             805..2715
                     /locus_tag="OO568_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6642584.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSLDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2773..3468
                     /gene="tsaB"
                     /locus_tag="OO568_003354"
     CDS             2773..3468
                     /gene="tsaB"
                     /locus_tag="OO568_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6642585.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3508..4089
                     /gene="yeaY"
                     /locus_tag="OO568_003355"
     CDS             3508..4089
                     /gene="yeaY"
                     /locus_tag="OO568_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6642586.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4294..5979
                     /gene="fadD"
                     /locus_tag="OO568_003356"
     CDS             4294..5979
                     /gene="fadD"
                     /locus_tag="OO568_003356"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6642587.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            6061..7176
                     /gene="rnd"
                     /locus_tag="OO568_003357"
     CDS             6061..7176
                     /gene="rnd"
                     /locus_tag="OO568_003357"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6642588.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRADWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMFNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHRKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="OO568_003358"
     CDS             complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="OO568_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6642589.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8251..8868)
                     /gene="yeaW"
                     /locus_tag="OO568_003359"
                     /pseudo
     CDS             complement(8251..8868)
                     /gene="yeaW"
                     /locus_tag="OO568_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(9210..9761)
                     /locus_tag="OO568_003360"
     CDS             complement(9210..9761)
                     /locus_tag="OO568_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6642590.1"
                     /translation="MRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTYPSLDR
                     LAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKEL
                     HSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSK
                     YGIYEQEGLVAPSQEPQDVKKAV"
     gene            9987..10214
                     /locus_tag="OO568_003361"
     CDS             9987..10214
                     /locus_tag="OO568_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6642591.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
BASE COUNT         2531 a         2488 c         2678 g         2573 t
ORIGIN      
        1 agttaacctg ttgacattca ggtgttttaa aggtaacagc tcatggtgtg ttatccagta
       61 agatggagaa aattaacggt cagcccgcac gctataacca gattgttcag ctaagaaaga
      121 aaaccagagt tgctaatcaa agggcgaaca caacggcatg gttatgttta tttatatagt
      181 tagttttatg gataaaattg aaattataaa tataaataat gaattagcag aagcatcaac
      241 agtgatatct tattctctgg tcgatttatg taaaaccgac cagtattatc gataaattat
      301 cgtctgactt attttgcttc gataaagctt acaccgcagc cacaatctta atttcaactt
      361 tatacttcgg gttcatcaaa cccgcttgta ccgtgcagcg caccggcgca tgacccgcga
      421 caacccaggc atcccacgct ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa
      481 tggtggcatc cagaatgctc gatttattgc tgccctgttt ttccagcacg gcgtcaatct
      541 gtgccagcgt gttggcggtt tgctcaaagg catcggcgtc gaggttttcc ggtacaccag
      601 tgtagtagag cgtgttgttg tggattacta catcagacca gcgggcttca gcatcgatac
      661 gaacgatagt catcattttg tcctcattat acttccattt tcgtgctgca aagactgcca
      721 caatgtgagg ttgccgtcac cacttcaact ggcgaaagcg ccctcatcct gacataatcc
      781 ctgttcaaat caacaggggg tagtgtgacg gacgattttg caccagacgg tcagctggcg
      841 aaagcgatac caggctttaa gccgcgagaa ccacagcgac agatggcggt agccgtcacc
      901 caggcgatag aaaaaggcca gccgctggtg gtggaagcag gaaccggtac gggcaaaacc
      961 tacgcttacc tggctcctgc gctgcgggcg aaaaagaaag tcattatctc gaccggctca
     1021 aaagcgttgc aggatcagct ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa
     1081 tatacgggca acgtggcgct gctgaaaggg cgctcaaact acctctgcct cgaacgtctc
     1141 gaacagcagg cgctggcggg gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg
     1201 ctgcgctcct ggtctaatca aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg
     1261 gaagattcac aggcgtggcc gctggtcacc agcaccaacg acaactgtct tggcagcgac
     1321 tgcccgatgt ataaagattg ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat
     1381 gtggtggtgg taaaccatca tctctttctg gcggatatgg tggttaaaga gagtggattt
     1441 ggcgagttga tccctgaagc ggacgtcatg atcttcgacg aagcccacca gctaccggac
     1501 attgccagcc agtattttgg tcagtcactc tccagtcgac aactgctcga cctggcaaaa
     1561 gacatcacca tcgcctaccg caccgaatta aaagacaccc agcagttaca aaagtgcgct
     1621 gatcgtcttg cccagagtgc gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc
     1681 ggtaacctgc gtgagctgtt agctaatccg caaattcagc gggcattttt actgctcgat
     1741 gacaccttgg aactttgtta tgacgtggcg aaactgtcac tggggcgttc cgccttgctg
     1801 gatgcggcat ttgagcgcgc cacgttgtat cgcacacggc tgaagcggct aaaagagatc
     1861 aatcagccgg gctacagcta ctggtacgaa tgcacttcgc gccattttac tctggctctc
     1921 acgccgctca gcgtggcgga taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg
     1981 atcttcacct cagcaacgct gtcggtgaac gacgatctgc atcatttcac ctcgcggctt
     2041 gggatagaac aggcggagtc gttgctattg ccaagcccgt ttgattacag ccgccaggcg
     2101 ttactctgtg tgccgcgcaa tctgccgcaa accaaccagc caggttctgc tcgccagtta
     2161 gcggcaatgc tgcgaccgat catcgaagct aacaacggtc gttgttttat gctttgtacc
     2221 tcgcacgcca tgatgcgcga tctggccgag cagttccgcg ctaccatgac gcttcccgtt
     2281 ttgttgcagg gggaaaccag taaaggccaa ctgttgcagc agtttgtcag tgccggtaat
     2341 gcgcttcttg tggcaaccag tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg
     2401 tcattggtaa ttatcgacaa attgccgttt acctcgctgg atgatccact gttaaaagcg
     2461 cgcatggaag attgtcgttt gcgcggtggc gacccgttcg atgaagtgca actaccagat
     2521 gccgtcatta ctctcaaaca gggggtaggt cgactgattc gcgacgccga cgatcgtggc
     2581 gtgctggtga tttgtgacta tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc
     2641 agtctgccgc ccgcgccacg cacccgtgac attgcccgag cggttcgttt ccttgcgata
     2701 ccatcctcca ggtaatttgt tacggagtat ggtatgatgc gcgccttttt attgatctaa
     2761 gcacgaagac ccatgcgaat tctggctatc gataccgcga cagaggcctg ctctgtcgcc
     2821 ctgtggaacg acggtactgt caacgctcat tttgagcttt gccctcgtga acatactcaa
     2881 cgaatcttac cgatggtgca ggatatcctg accaccagcg gaacttccct gactgatatt
     2941 aacgctctgg cttacgggcg cggccccggt agctttaccg gcgtgcgcat tggtattggc
     3001 atcgcgcaag ggctggcgct tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg
     3061 acgatggcgc aaggggcgtg gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgac
     3121 gcgcgaatgg gcgaagttta ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac
     3181 ggtgaagaaa ccgaagccgt actcaaaccc gaactcgttc atgaacgaat gcaacagctt
     3241 tccggcgaat gggtgacggt agggacgggc tggcaagcct ggccggatct cggtaaagag
     3301 agcgggctgg ttttgcgcga tggcgaagtg ttactccccg ctgcagaaga tatgctgccg
     3361 attgcttgtc agatgtttgc cgagggtaaa acggtggcgg tggaacatgc cgagccggtt
     3421 tatttgcgta acaacgtcgc atggaagaaa cttccgggca aagaatgaat cttaagagta
     3481 tgccctgaga aaaaaaggag tcgcatcatg gcggttcaaa agaatgttat caaaggcata
     3541 ctggcaggta cgtttgcgct aatgctgagt ggttgtgtca ctgtgccgga cgccattaaa
     3601 ggcagcagtc ccacgccgca acaagattta gttcgggtga tgagtgcgcc gcagctgtac
     3661 gttggtcagg aggcacgctt tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa
     3721 acccgcctgg aaattgctac cgtaccgctg gacagcggag ctaggccgac gctgggagaa
     3781 ccttctcgcg ggcgcattta tgccgatgtg aacggttttc tggacccggt ggatttccgt
     3841 ggacaactgg ttacggtagt cgggccaatc accggtgcgg ttgacggcaa aatcggcaat
     3901 acgccctata aatttatggt gatgcaagta acgggttaca aacgttggca tttaacccag
     3961 caggtgatta tgccgcctca gccgattgat ccatggtttt atggcggtcg tggctggccc
     4021 tatggctacg gcggatgggg ctggtataat cccggccccg cgagagtaca aacagttgta
     4081 accgaataat tgcttgtttt taaagaaaaa gaaacagcgg ctggtccgct gtttctgcat
     4141 tcttacggta aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt
     4201 atcaagctgg tatgattagt taatattatg ttaacagcgt gtatatcatt tggggttgcg
     4261 atgacgacga acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat
     4321 cccgcggacg ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt
     4381 gagcagtcgg tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg
     4441 accttccgca agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg
     4501 gggctgaaga aaggcgatcg cgttgcgttg atgatgccta acttattgca atatccggtg
     4561 gcgctatttg gcattttgcg tgccgggata atcgtcgtaa acgttaaccc gttgtatacc
     4621 ccgcgtgagc ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct
     4681 aactttgccc acactctgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg
     4741 acccgtatgg gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa
     4801 tacatcaagc gcttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca
     4861 ctgcataacg gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc
     4921 ttcctgcaat acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc
     4981 aatatgctgg cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt
     5041 aaagaactgg tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc
     5101 ctgctgttta tcgaactggg tgggcagaac ctgcttatca ctaacccgcg tgatattcca
     5161 gggttggtaa aagagttagc gaaatatccg tttaccgcta tcactggcgt taacaccttg
     5221 ttcaatgcgt tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt
     5281 tccgcaggcg gtgggatgcc tgttcagcaa gtggtggcag aacgctgggt gaaactgacc
     5341 ggacagtatc tgctggaagg ttatggcctg accgagtgcg cgccgctggt gagcgttaac
     5401 ccgtatgata ttgattatca tagcggtagc atcggtttgc cggtgccatc gacggaagcc
     5461 aaactggtgg atgacgacga caacgaagta ccaccgggtc aaccgggtga gctttgtgtc
     5521 aaaggaccgc aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc
     5581 aaaaatggct ggttacacac cggcgacatc gcggtgatgg atgaagaagg attcctgcgc
     5641 attgtcgatc gtaaaaaaga catgattctg gtttccggtt ttaacgtcta tcccaacgag
     5701 attgaagatg tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct
     5761 tccggctcca gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc
     5821 gaagagtcac tggtgacttt ttgccgccgt cagctcacgg gatacaaagt accgaagctg
     5881 gtggagtttc gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta
     5941 cgtgacgaag cgcgcggcaa agtggacaat aaagcctgag cgttaagtca gtcgtcagac
     6001 gccggttaat ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa
     6061 atgattacta cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgc ctttccggcg
     6121 atagccctgg atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt
     6181 caacttttcg atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca
     6241 ccgctgaaag cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa
     6301 gatctggaag tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa
     6361 atccttgctg ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag
     6421 tattccggcg ttacgctgga caagagtgaa tcgcgcgccg actggctggc cagaccgctg
     6481 accgaacgtc agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcactgcc
     6541 aagcttatgg tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc
     6601 ctgatgcaaa tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc
     6661 aatgcctggc agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga
     6721 ctgcgcaagg cgcgagagcg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg
     6781 tggtcggtcg cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc
     6841 ggtagcgaaa tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca
     6901 ttgccggaag atgccttacc gcagccgatg tttaacctga tggacatgcc tggttatcgt
     6961 aaagcgttta aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc
     7021 gccgaattgc tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ccggaaactg
     7081 aaaccgcaga acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa
     7141 gcattacaca atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg
     7201 cgctcagcgc cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc
     7261 gcacgcgaac aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac
     7321 tgatcccgat ggtcagcttc accttccagt attgccgttt cgcaggttcc gcatacccct
     7381 tcacgacata aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc
     7441 atctcttccg gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg
     7501 ccggttttgt cttcgatagc aaattgctca aagtgcagcg tatcggcggc gatgtccaga
     7561 cgcgcagctt cacttcttac cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt
     7621 gtgccaggtt cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgaa
     7681 tgatgcaaat ggactttctc agcctgcgga tgctggacta gctcatcacg aaatgcgcaa
     7741 ctttctggat ttcgcgagca gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct
     7801 gccatgtgcg acaggaaagg ggtgatacca ataccgcccg cgataaacag atgctttctg
     7861 gctgagggaa ttagcgcaaa caggttatta ggcgttgaga tcgttaaccg atcgcccact
     7921 tttacctgct gatgcaaaaa gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca
     7981 atctgataac tagaggtgtt atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg
     8041 ttatcaccat cgctcatctg cactaggatg tgacttcctc cggtgaacgc aggcagcggt
     8101 ttgccatcgg ttgccaccag cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg
     8161 ctcacctgta cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc
     8221 cagcggcgga aaataccgcc gccgatgtca ttagtcctta aacacctgcg ccagcagatt
     8281 atggaaatgg gcgataccat gttcggaaat gccactgccg ctactgtcgg ccatgatgcg
     8341 cccctgacca cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa
     8401 atcttccgga tggaacacat cgcgatacca ctcaatcagc gatttttgct cgttggttaa
     8461 ctcttcattg gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa
     8521 ttcataaatg accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca
     8581 cagccagaaa ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga
     8641 ctgtttggcg aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc
     8701 aacctgtacg gagtcggaga aacctagatg cgccggacca cagtgatagc actcgagata
     8761 gttatcgaca atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt
     8821 cagattgtgg acttccgggc aggcttccag cactttcgcg cccagaccgg tgtattgatt
     8881 cacttgaagt acgaaaaaaa accgggagga cattggatta ttcgggttct gatgggatta
     8941 gatttggtgg ggcttgcaag cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga
     9001 aagatagtaa aaaattgctt ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat
     9061 catttcaata acagcttcaa aaaacgttca aaaccgataa caaccaggct gtaaccaaat
     9121 gactcatatc acaaatcagc tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg
     9181 tgaagtatgt tttagctggt tcatggttgt tatacggctt tttttacatc ttgtggttct
     9241 tgtgaaggag ctacaagacc ttcctgttca taaataccat actttgacaa aatcttttta
     9301 cgagtatctg ttggtaatga ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata
     9361 ccttgcaaat cagcctggat ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca
     9421 aatcctttct ttctgaacaa ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg
     9481 acaattgcga tcagtttagt tttcaatgtg ctgcggtcag gttgagaatt tttattctct
     9541 tgattatttt tatgctcaac cattggcatg ctctgacttt ggataaacca ctctattgga
     9601 cgccctgttg cgtaggctat ttgtgctaat cgatctaaag acgggtatgt cttgccagac
     9661 aggtagtttc tgataacccc atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat
     9721 tctccattca ttgcctcttc aaggcgttcc ttgaaacgca ttatttccgg ggcataaaaa
     9781 tgcgctctgc atttttgctt tctttttcac ctgtcatgct caatctctct gttatataaa
     9841 gaattttaca gaattctttg atttgcattg gttaaaataa aaatgtgcat agctcttgaa
     9901 atgcgcattt ttctgcgata tcgtttttcc cgatagcggt agccggtatc agttaccgaa
     9961 aacgttaaga gagtaaggat cgcagaatgt cagcaaaaaa ccaagactgg cacgcagagc
    10021 agataaaggc tgctgttcgt atgaagggga agacactatc tcaattatcc agagaggcag
    10081 gcttaaagcc tgacactatg cgtaacgtat ttcgctgcca tataccacga tacgaagcaa
    10141 tcattgctag atatttggat atggaaccag cccagatttg gccttctcgg tatatggaga
    10201 aaacatcatg ttaacccatt gggtaacagc gcaagaatgc gtaggaatgt catgctttcc
    10261 taaacatgag
//
LOCUS       DAJTYG010000116        10189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTYG010000116 DAJTYG010000000
VERSION     DAJTYG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.116"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            80..883
                     /gene="hpaI"
                     /locus_tag="OO568_003362"
     CDS             80..883
                     /gene="hpaI"
                     /locus_tag="OO568_003362"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6642592.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALTARFGNSSSISMAKQNNNSVY"
     gene            905..2260
                     /gene="hpaX"
                     /locus_tag="OO568_003363"
     CDS             905..2260
                     /gene="hpaX"
                     /locus_tag="OO568_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6642593.1"
                     /translation="MTTSSLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMTIFWTTQDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            2270..3160
                     /gene="hpaA"
                     /locus_tag="OO568_003364"
     CDS             2270..3160
                     /gene="hpaA"
                     /locus_tag="OO568_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6642594.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFTRLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSPEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            3187..4124
                     /locus_tag="OO568_003365"
                     /pseudo
     CDS             3187..4124
                     /locus_tag="OO568_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4351..5909
                     /gene="hpaB"
                     /locus_tag="OO568_003366"
                     /pseudo
     CDS             4351..5909
                     /gene="hpaB"
                     /locus_tag="OO568_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            5927..6439
                     /locus_tag="OO568_003367"
     CDS             5927..6439
                     /locus_tag="OO568_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6642595.1"
                     /translation="MQLDEQRLRFHDAMVSLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGESRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            6832..8982
                     /gene="btsT"
                     /locus_tag="OO568_003368"
     CDS             6832..8982
                     /gene="btsT"
                     /locus_tag="OO568_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6642596.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            9032..9235
                     /locus_tag="OO568_003369"
     CDS             9032..9235
                     /locus_tag="OO568_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6642597.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            9246..>10189
                     /gene="yjiA"
                     /locus_tag="OO568_003370"
     CDS             9246..>10189
                     /gene="yjiA"
                     /locus_tag="OO568_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6642598.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAG"
BASE COUNT         2422 a         2659 c         2637 g         2471 t
ORIGIN      
        1 agccccgaaa aagagccagt cagttgctta actgactggc attactccat tagctgccgc
       61 tttgtttaag gagagaacga tggaaaacag ttttaaagcg gcgctgaaag caggccgtcc
      121 gcagattgga ttatggctgg ggctgagcag cagctacagc gcggagttac tggccggacc
      181 aggattcgac tggttattga tcgacggtga gcacgcgccg aataacgtgc aaaccgtgct
      241 cacccaacta caggcgattg cgccctaccc cagccagcca gtggtacgcc catcgtggaa
      301 cgatccggtg caaatcaaac aactgctgga cgtaggcaca caaaccttac tggtgccgat
      361 ggtacaaaac gccgacgaag cccgtaaagc ggtacgcgcc acccgttatc cccccgccgg
      421 tattcgcggt gtgggcagtg cgctggctcg cgcctcgcgc tggaatcgca ttcctgatta
      481 cctgcaaaaa gccaacgatc aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat
      541 gaagaactta ccgcagattc tggacgtgga aggggtcgac ggcgtgttta tcggcccggc
      601 ggatctgagc gccgatatgg gttatgccgg taatccgcag cacccggaag tacaggccgc
      661 cattgagcag gcgatcgtgc agatccgcga agcgggcaaa gcgccgggga tcctgatcgc
      721 caatgagcaa ctggcaaaac gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt
      781 tgacaccacc ctgctcgccc gcgccgccga agcgctgaca gcacggtttg gtaatagttc
      841 ttctatttcg atggcgaagc aaaacaacaa ctctgtctac taaacgtacg atgtggagcg
      901 catcatgacg acctctagcc tgcaatataa agataataaa actgttgaaa ttgaacatcg
      961 tgtcattaat aaattatttc gtcgcctaat catttttctt tttattctgt ttgttttttc
     1021 gtttcttgat cgcatcaata taggatttgc cggactgaca atgggcaagg atcttggcct
     1081 gacgtccaca atgtttggcc tcgccgcgac actcttttat gtcacgtacg tcatgtgcgg
     1141 gatccccagt aatatgatgt tagcgaaaat tggcgctcgt cgatggatcg ctgggatcat
     1201 ggtggtctgg ggaattgctt ccacctgcac aatgtttgca acaagccccg aaacactcta
     1261 tctactgcgt atgctggttg gtattgctga ggcgggtttt ttacctggta ttttggttta
     1321 tctgacctgg tggttcccgg catatcatcg ggccagagcc aatgccttat ttatgatagc
     1381 gatgccagtc actatgatgc tgggattgat cctttcaggc tacattctgg ccttggatgg
     1441 actgtggaat ctaaaaggct ggcaatggct ctttttgctg gaaggtctgc catcggtcat
     1501 tcttggtgtc gtcacctggt tttatctcaa cgacacacca gaccaggcga cctggcttga
     1561 tgaaaacgaa aaacaggctt taaaaacgat gattctccgt gaacaagaga gtgcatttgc
     1621 ccatgcatct gttccacgct caaccttacg tgaagtcctg accccagcca tcatgctata
     1681 tacacttgct tatttttgcc tgactaatac tctgagtgcg attaatatct ggacgccgca
     1741 aatcctacag agtttcaata ctggcagtag caacatcatg attggtttgc tggcggccat
     1801 cccccaattc tgcacaattt ttggcatgat ctggtggagt cgtcgttccg accgtcttaa
     1861 agaacgcaaa aagcatacga ttcttcccta cctgtttgcc gctgccggat ggttactcgc
     1921 ttctgcgaca gatcacagcc tcattcaact gagcggcatc attatggcct caatgggatc
     1981 atttaccgcg atgactatct tttggacaac acaggatcaa gttataagct tgcaatcgcg
     2041 tgccgttgcg atggctgtca tcaatgctgt cggcaatgta ggttcagcag tcagtccatt
     2101 acttatcggt attttgaaag atgcaaccgg cagttttagc tcaggtttgt ggtttgtagc
     2161 aggattgctg gtactgggcg cgttagtcat gacccgaatc cctatgggga gtcgggaaca
     2221 ctctctttca tctgaactta tcgcaccaaa accacaataa ggagtcatga tgtccagtga
     2281 agctatcttt gaacatggtt atgaaaatat tgatttggtc cgggaatatg atgcccgcta
     2341 cgctggtgaa gatgtgcatt atgaaacatt tacccggtta gcgaatttct ttggacgcga
     2401 tatgcgccct cactggcacg atagatattt tcaactccat tatctggtca ctggacgcat
     2461 tactttacaa ctggatgagc acttctattc tgtgcgcgca ccgctttttg tactaacacc
     2521 accatcggtg ccacatactt tctttactga tcctgatagc gatgggcatg ttctgaccgt
     2581 acgtcaggag ttaatttggc ccctggtaga aaagctctgg cctggtagtg gagatgccgc
     2641 aatgctacaa ggtatctgtc tgtcaccgga gggaatggaa catacactgg cggcattaaa
     2701 tcattattgg ccgataatat ccctggagtt tcaacaaaat aagccaggac gggaaattct
     2761 attgcaatca ctggcgcaaa gtatttttac aatcctactg cgtgaagcac caccaaatga
     2821 tatttcaaca tgcagcgtgc gcggcgagat gtgtttattc cagaagttta atcggctgat
     2881 tgacgaaaat tatcgccaac atttaatggt ccctgaatat gcaacaatgc tcggtatgag
     2941 tgaatcacgg cttaccgagc tgtgccgacg ttttgccaat caatcgccga agagattgat
     3001 tttcgaacga gtgagccggg aagccagacg gcttctgctc tatagcgagc aaagcatcaa
     3061 ccagatagcc cttgatcttg gctacaaaga ccccgcctat ttcgcccgtt ttttcaaccg
     3121 aatgatgggt tgctcaccaa cacaattccg tggtcgctga ccatttatca acctgagaga
     3181 ctcgacatga aaatatcttt acgctcacta tttgtggcgt gcctgattgc gccagtggca
     3241 ttcacctacg cctccgaacc gcaataaatg aaaattgtac tacttggaac gaaaggtggc
     3301 ccttctttat taaaagctga ccgtttaccg atggcaacgg cacttattat tggcaataaa
     3361 acctggttaa ttgatgctgg ttacggggcc agcttacaac tggtaaaaaa taatattcca
     3421 ctgcgccaga ttgataatat tttactcacc catttacaca gtgatcatat tctggactat
     3481 ccatcagtct taatgaatgc atgggctagc ggtctgcagg acaaaactat tcatgtattc
     3541 ggtccgccag gcacccgagc catgaccgct gccagttggc aggtcttcga tcgggacatc
     3601 acactgcgta tggaagaaga aggaaaaccc gatcctcgca aactcgttac agctcaagat
     3661 gttggtgaag gcgtagttta taaagatgat accgtgaccg tgactgcatt aaaggtccct
     3721 cattctcctt tccctgacgg tgaggctttt gcttatcgct tcgacacaca aggcaagcga
     3781 attgtcttct caggagatac ctcgtacttt cccccactgg caactttctc agaaggcgct
     3841 gatatacttg tgcatgaggc cgtacatgag ccctccgtcg ctaagctggc agacagtatt
     3901 ggcaatggta aaacactggc aaaagccatt gcttctcacc atacgactat tgaggatgta
     3961 ggcaaaattg ccaatgaagc aaaagtgaaa aaactcatcc ttagccacct ggtaccaatc
     4021 accgttccgg atgaaacatg gaagcaagaa gcacagaaaa ctattcaggc cctatcgtcg
     4081 ttggtcatga aaatatgacg attaccattc cattcgcccc ataaaaaatt ccgtccccgt
     4141 tagcccttgt tagcggggaa tattatgatt tgccttactg taagttgatc acgatcgcag
     4201 tttactattt aactccacct tgctgtcgaa aagtaccaac gttcgcctga accgtctctt
     4261 cccgacaacc gcgttcaacg cttcaattaa ataacaaaaa caatacacaa aattaacaat
     4321 cgtattccga ttaatactgt agaggtcgac atgaaaccag aagatttccg cgccagtacc
     4381 caacgtcctt tcaccgggga agagtatctg aaaagcctgc aggatggtcg cgagatctat
     4441 atctatggcg agcgagtgaa agacgtcacc actcatccgg catttcgtaa tgcggcagcg
     4501 tctgttgccc aactgtacga cgcgctgcac aaaccggaga tgcaggactc tctgtgttgg
     4561 aacaccgaca ccggcagcgg cggctatacc cataaattct tccgcgtggc gaaaagtgcc
     4621 gacgacctgc gccagcaacg cgacgccatc gctgaatggt cacgcctgag ctatggctgg
     4681 atgggccgta ccccagacta caaagccgct ttcggttgcg cactgggcgc gaatccgggc
     4741 ttttacggtc agttcgagca gaacgcccgt aactggtata cccgtattca ggaaactggc
     4801 ctctacttta accacgcgat tgttaaccca ccgatcgtca tttgccgacc gataaagtga
     4861 aagacgttta catcaagctg gaaaaagaga ctgacgccgg gattatcgtc agcggtgcga
     4921 aagtggttgc caccaactcg gcgctgactc actacaacat gattggcttc ggctcggcac
     4981 aagtgatggg cgaaaacccg gacttcgcac tgatgttcgt tgcgccaatg gatgccgatg
     5041 gcgtgaaatt aatctcccgc gcctcttatg agatggtcgc gggtgctacc ggctcgccat
     5101 acgactaccc gctctccagc cgcttcgatg agaacgatgc gattctggtg atggataacg
     5161 tactgatccc atgggaaaac gtgctgatct accgcgattt tgatcgctgc cgtcgctgga
     5221 cgatggaagg cggtttcgcc cgtatgtatc cgctgcaagc ctgtgtgcgc ctggcagtga
     5281 aactcgactt cattacggca ctgctgaaaa aatcactcga atgtaccggc accctggagt
     5341 tccgtggtgt gcaggccgat ctcggtgaag tggtggcgtg gcgcaacacc ttctgggcat
     5401 tgagtgactc gatgtgttca gaagcgacgc cgtgggtcaa cggggcttat ttaccggatc
     5461 atgccgcact gcaaacctat cgcgtactgg caccaatggc ctacgcgaag atcaaaaaca
     5521 ttatagaacg caacgttacc agtggcctga tctacctccc ttccagtgcc cgtgacctga
     5581 acaatccgca gatcgaccag tatctggcga agtatgtgcg cggttcgaac ggtatggatc
     5641 acgtccagcg catcaagatc ctcaaactga tgtgggacgc cattggcagc gaatttggtg
     5701 gtcgtcacga gctgtatgaa atcaactact ccggtagcca ggatgagatt cgcctgcaat
     5761 gtctgcgcca ggcacaaagc tccggcaata tggacaagat gatggcgatg gttgatcgct
     5821 gcctgtcgga atacgaccag aacggctgga ctgtgccgca cctgcacaac aacgacgata
     5881 tcaacatgct ggataagctg ctgaaataac gcagcaggag gttaagatgc aattagatga
     5941 acaacgcctg cgctttcatg acgcgatggt cagcctgtcg gcagcggtaa atattatcac
     6001 caccgagggc gacgccggac aatgcgggat tacggcaacg gctgtctgct cggtcacgga
     6061 tacaccaccg tcgctgatgg tgtgcattaa cgccaacagt gcgatgaacc cggtttttca
     6121 gggcaacggc aagttgtgcg tcaacgtcct caaccatgag caggaactga tggcgcgcca
     6181 cttcgcgggc atgacaggca tggcgatgga agagcgtttt agcctctcat gctggcaaaa
     6241 aggtccgctg gcgcagccgg tgctaaaagg ttcgctggcc agtcttgaag gagagagccg
     6301 cgatgtgcag gcgattggca cacatctggt gtatctggtg gagattaaaa acatcatcct
     6361 cagtgcagaa ggtcacggac ttatttactt taaacgccgt ttccatccgg tgatgctgga
     6421 aatggaagct gcgatttaag ctccagcccc ttcacgcatt cgtcgaaggg gcatttttat
     6481 tgtgatctca atcacatatc tacccgatga ataattcttg cccaaccgct caccgccatt
     6541 cctgaccgct tacatcccta aaataaccac tcagttattt accttacttt acgcgcacgt
     6601 atctctggca acatcactgc aggatagcgg tcaatttacc tcctcaaacg caacgcaaac
     6661 ctagaacggc ttcggcctat gattaacaaa ttactctatg gattttggtt tgcaggaagg
     6721 cagcaagtga gtgaatcccc gggagcttac aaaagtaagt gtcagaggtg aacgaacgca
     6781 gctgccgcac ctgtaagcca aaagacgacg agtaaaatgc caggttttac tatggatacg
     6841 aaaaaactat tcaagcacat accctgggtg attctcggaa tcatcggtgc attctgcctc
     6901 gcggtagttg cattacgtcg gggggagcac gtcagcgccc tgtggatcgt ggtcgcctct
     6961 gtgtcggtgt atctggtggc gtatcgctac tacagtctgt acatcgccca gaaggtgatg
     7021 aaactcgacc ccacgcgcgc gacgcctgcg gttattaaca acgacggtct gaactacgtt
     7081 ccgaccaacc gttacgtgtt gtttggtcac cacttcgccg ctatcgccgg tgctggtccg
     7141 ctggtgggac cggttctcgc cgcgcagatg ggctacctgc ctggcacgct gtggctgctg
     7201 gcgggggtag tgctggccgg tgcggtacag gactttatgg tgctgtttat ctcctctcgc
     7261 cgtaacggcg catctctcgg tgagatgatc aaagaagaga tggggccagt accggggact
     7321 atcgcgctgt ttggctgttt cttaatcatg atcatcatcc tcgccgttct ggcgctgatt
     7381 gtggttaaag ccctggccga aagtccgtgg ggtgtcttca ccgtttgctc aaccgtaccg
     7441 attgcgctgt ttatgggtat ctacatgcgc tttatccgtc cggggcgtgt gggtgaagtc
     7501 tctgttattg gtatcgtgct gctggttgcc tctatctact tcggtggcgt gattgctcac
     7561 gatccgtact ggggtccggc actgaccttt aaagacacca ccattacctt cgcgctgatt
     7621 ggctatgcgt ttgtttccgc actgctgccg gtgtggctga tcctcgcacc gcgcgactat
     7681 ctggcaacct tcctgaaaat cggcgttatc gtcggcctgg cgctgggtat cgtggtgctg
     7741 aacccggaac tgaaaatgcc tgcgatgacc cagtacattg acggtactgg cccgctgtgg
     7801 aaaggcgctc tgttcccgtt cctgttcatc accatcgcct gtggtgcggt atctggcttc
     7861 cacgcgctga tctcttccgg tacgacgccg aaactgctgg ctaacgaaac cgacgcgcgt
     7921 ttcattggct acggcgcaat gctgatggag tccttcgtgg cgattatggc gctggttgca
     7981 gcgtccatca tcgaaccggg tctttacttc gcgatgaaca ccccgcctgc tggccttggc
     8041 atcaccatgc ctaacctgca tgaaatgggt ggcgagaacg cgccgatcat catggcgcag
     8101 ctgaaagacg ttaccgcaca cgcggcagcg accgtcagct cctggggctt cgtgatttcg
     8161 ccagagcaga tcctgcaaac cgcgaaagac attggtgagc cttctgtcct gaaccgtgca
     8221 ggtggtgcgc cgacgctggc ggtaggtatc gctcacgtgt tccacaaagt gctgccgatg
     8281 gctgacatgg gcttctggta tcacttcggt attctgttcg aagccctgtt catcctgacc
     8341 gcgctggatg cgggtacccg ttctggccgc tttatgctgc aagacctgct gggtaacttc
     8401 atcccgttcc tgaaaaaaac cgactctctg gttgccggta tcatcggtac tgcgggctgt
     8461 gtgggtctgt ggggctacct gctgtatcag ggcgtggtcg atccgctggg cggcgttaag
     8521 agcctgtggc cgctgttcgg tatctccaac cagatgctgg cagccgtagc gctggtactg
     8581 ggcaccgttg tgctgattaa gatgaagcgc acccaataca tctgggtaac tgttgttccg
     8641 gctgtatggc tgcttatctg caccacctgg gcgctgggtc tgaaactgtt cagcaccaac
     8701 ccgcagatgg aaggcttctt ctacatggca agccagtaca aagagaagat tgctaacggt
     8761 actgacctga cggcgcagca gattgccaac atgaaccaca tcgttgtgaa caactacacc
     8821 aacgcaggcc tgagtattct gttcctgatt gtggtgtaca gcatcatctt ctacggtttc
     8881 aaaacctggc ttgcggtgcg taacagcgac aaacgtactg acaaagaaac accgtacgtt
     8941 ccaatcccgg aaggcggcgt gaagatctct tcgcaccact aaccgtgttt agccccgctt
     9001 cggcggggct ttgttcttat cagagtgaac tatgtttggt aacttaggac aggcaaaaaa
     9061 atatctcggc caggcggcga agatgttgat tggtattcca gactacgaca actacgtcga
     9121 gcatatgaag accaaccatc ccgacaagcc gtacatgagc tatgaagaat tcttccgcga
     9181 acgccagaac gcgcgctacg gcggcgatgg taaaggcggt atgcgctgtt gttaatggag
     9241 agagcatgaa cccgattgca gttaccctac tcaccggttt tttaggcgcg ggcaaaacta
     9301 ccctgctgcg ccatattctt aacgaacaac acggctacaa gattgccgtg attgaaaacg
     9361 aattcggcga agtctccgtt gatgatcaac tgattggcga ccgcgccacg cagatcaaaa
     9421 cgctgaccaa cggctgcatc tgctgttcgc gctccaacga gctggaggac gcactactcg
     9481 acctgctgga taatctcgac aagggcaata ttcagttcga tcgtctggtg attgaatgca
     9541 ccggcatggc cgatcccggc ccgattattc agaccttttt ctcccatgag attttatgcc
     9601 agcgctacct gctggacggc gtgattgcgc tggtggatgc ggtacatgcc gatgagcaga
     9661 tgaaccagtt caccatcgcc cagtcccagg tgggctacgc cgaccgtatt ctgctgacta
     9721 aaaccgacgt cgcaggcgaa gcagagaaac tgcgtgaacg cctggcgcgc atcaacgccc
     9781 gcgcaccggt ctacaccgtc acccacggcg acatcgacct gggcctgctg ttcaacacca
     9841 acggttttat gctggaagag aacgtcgtca gcaccaaacc gcgtttccac tttatcgcgg
     9901 ataaacaaaa cgatatttcg tcaattgtgg tggaactgga ttacccggta gatatcagcg
     9961 aagtttcccg cgtgatggaa aacctgctgc tggaatcggc ggataaactg ctgcgttaca
    10021 aagggatgct gtggattgac ggcgaaccta accgcctgct gttccagggc gtccagcgcc
    10081 tctacagcgc cgactgggac aggccgtggg gcgatgaaaa accgcatagc acaatggtgt
    10141 ttatcgggat tcagttgccg gaggaagaga ttcgggcggc gtttgcggg
//
LOCUS       DAJTYG010000117        10086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTYG010000117 DAJTYG010000000
VERSION     DAJTYG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.117"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(296..1156)
                     /locus_tag="OO568_003371"
     CDS             complement(296..1156)
                     /locus_tag="OO568_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR6642599.1"
                     /translation="MALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFD
                     LNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNEGTPIWACANVDARA
                     AREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSDIYRQPSTITMISDW
                     LAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADILSPVKETGTLLGVVS
                     SQAAELCGLKAGTPVVVGGGDMQLGCLGLGVVRPAQTAVLGGTFTDNKQCTLSEARHQ
                     LPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO568_003372"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO568_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR6642600.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="OO568_003373"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="OO568_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR6642601.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAGTERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSSKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAEDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="OO568_003374"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="OO568_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR6642602.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="OO568_003375"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="OO568_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR6642603.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWFWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="OO568_003376"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="OO568_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..7965
                     /gene="lsrF"
                     /locus_tag="OO568_003377"
     CDS             7090..7965
                     /gene="lsrF"
                     /locus_tag="OO568_003377"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR6642604.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            7989..8279
                     /gene="lsrG"
                     /locus_tag="OO568_003378"
     CDS             7989..8279
                     /gene="lsrG"
                     /locus_tag="OO568_003378"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR6642605.1"
                     /translation="MHVTLVEINVHEDKVEEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8318..9076
                     /gene="tam"
                     /locus_tag="OO568_003379"
     CDS             8318..9076
                     /gene="tam"
                     /locus_tag="OO568_003379"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR6642606.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9080..9994)
                     /locus_tag="OO568_003380"
     CDS             complement(9080..9994)
                     /locus_tag="OO568_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR6642607.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTYISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         2437 a         2484 c         2655 g         2510 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgcagtaaca ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcatg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatggctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt caagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgttctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 ggaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cagcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcgg aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tagaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgc ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atccccaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggt tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gcattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcta gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgctggattg gggaatgcag
     7201 tcacgcttat cgtggatatt taatccgaaa acgggtaaaa ctgtgatgct ggcctttgac
     7261 catggttatt ttcaggggcc gactaccgga cttgaacgca ttgatataaa tatcgccccg
     7321 ctgtttgaac atgccgatgt attaatgtgt acgcgcggca ttttgcgcag cgtagttccc
     7381 ccggcgacca ataagccggt ggtactgcgg gcgtcaggtg cgaactctat tctggcggaa
     7441 ttaagtaatg aagccgtggc gttatcgatg gatgatgccg tgcgcctgaa cagttgcgcg
     7501 gtggcggcgc aggtttatat cggcagcgaa tatgaacatc agtcgatcaa aaatattatt
     7561 cagctggtcg atgccggaat gaaagtggga atgccgacca tggccgtgac tggcgtgggc
     7621 aaagatatgg tgcgcgatca gcgttatttc tcgctcgcga ctcgaatcgc cgctgaaatg
     7681 ggggcgcaaa ttatcaaaac ctattatgtc gaaaaaggtt ttgaacggat tgttgccgga
     7741 tgtccggtac ccattgttat tgctggcggt aaaaaattac cggagcgcga ggcgctggaa
     7801 atgtgctggc aggctatcga tcagggcgct tctggtgtgg atatggggcg taatattttc
     7861 cagtctgacc atccggtggc gatgatgaaa gccgtacagg cggtggttca ccataacgaa
     7921 acggctgatc gggcatatga actctatctg agtgaaaaac agtaactgcg gatttaagga
     7981 gaagaattat gcacgtcaca ctggttgaaa ttaacgttca tgaagacaag gttgaagagt
     8041 ttatcgaagt ttttcgccag aaccacctgg gctctgtaca ggaagaaggt aatttgcgct
     8101 tcgatgtctt acaggacccg gaagtgaatt cgcgctttta tatttacgaa gcctataaag
     8161 atgaagacac agtggcattc cataaaacca cgcctcacta caaaacctgt gtcgcgaaac
     8221 tggaatcttt aatgaccggg ccgcgtaaaa aacgtctgtt caatgggttg atgccgtgag
     8281 gctacaattg gggtaacgcg ctgacgggag taaaaaaatg tctgactgga acccctcttt
     8341 atatctgcac ttttccgctg aacgatcgcg tccggcggtg gagctgcttg ccagagtgcc
     8401 gctggagaat gtcgaatatg ttgccgatct tggctgtggc ccaggtaaca gcaccgccct
     8461 gctgcatcaa cgttggcctg cggccaggat aacaggcatc gactcgtctc cggcgatgat
     8521 tgctgaagcg cgcagtgctt tgccagactg ccagttcgtg gaagcagaca tccgcaactg
     8581 gcaaccggaa caggcactcg atctgatttt tgctaatgcc tcactgcaat ggctgcccga
     8641 ccactacgaa ttgtttcctc acttggtttc tttacttaat ccgcagggcg tgctggcagt
     8701 acagatgcca gataactggc tggagccgac ccatgtgctc atgcgcgaag ttgcttggga
     8761 acaaaactac ccggatcgcg ggcgtgagcc gttggctggc gttcatgctt actacgatat
     8821 tttgagcgaa gccggatgtg aggtcgatat ctggcgaacc acttactatc accagatgcc
     8881 gtcgcaccag gcgattatcg attgggtgac cgccactgga ttacgtccgt ggttacagga
     8941 tctgaccgag agcgaacagc agctttttct tacgcgctac catcagatgc tggaagagca
     9001 gtatccactg caagagaacg gacagatact gctggcattt ccgcgtctgt ttattgttgc
     9061 ccgccgtacg gagtaattat cacgtcagct ggtaatgacg atcgggaaga atttttgctg
     9121 gaatttctgc ttcatcgttc atctgtaaca ggtcaatttc aatagcgttg cagatggcat
     9181 ccagcggtaa atcattgttt tcagtaccga acggatcttc cagttcttcc gccagacaat
     9241 ccagcgaaat ataagtgtag gaaatcagca cagagataaa aggcgtcatg taatgcaggt
     9301 ccacgaccag cgcgaacggc agcatgatac aaaacagata aacggtacga tgcaaaatca
     9361 gggtgtaggc aaagggaatt ggcgtgtagg caatgcgctc acagcctgcc agaaccgctg
     9421 aaatatcatt aagccgatcg ttgaggctaa taaacaggat atctgaaagc tgtccattgc
     9481 ggcgctgaac cgccaaccat tctcccatta ttaacaagat acggttagct ggagagttcg
     9541 aagccagtac acgctgaaga tctccagtct tgagataatg agccagcact tccgcctgtg
     9601 gctgtttgcg taatgtcatg cgtaaacagt gggcgaaggc gatttgcagc ctggcaaact
     9661 cccttacact tgccgaatcc ggcaatgtcg tttttacctc tcgcagtaac gaccgtgagg
     9721 caatcatcaa ctgcccccaa agttttcgcg cttcaacgta acgggcgtac ccggcattat
     9781 tacgaaaacc aagaaaaatg gcgatggcga caccgagaat gctgaacggt gcgagggtga
     9841 atttgatgcc cagatgcgtg taccagggca gcatgaatat aacagcaata gaaaagagaa
     9901 aattgagtag taagcgcgag gatatcttgg ataatactga gccgtgccag acaaaaatac
     9961 ggcgcagcca gtgttgttgt ggacgaacaa tcatggttat cttcaggcgt ggaaaagtcg
    10021 ccctattaaa cgtgattacg atcacattct caagacgcta cttacaaatt acctagaacc
    10081 acggca
//
LOCUS       DAJTYG010000118        10042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTYG010000118 DAJTYG010000000
VERSION     DAJTYG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.118"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..807
                     /locus_tag="OO568_003381"
     CDS             <1..807
                     /locus_tag="OO568_003381"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6642608.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            963..2498
                     /gene="gadC"
                     /locus_tag="OO568_003382"
     CDS             963..2498
                     /gene="gadC"
                     /locus_tag="OO568_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6642609.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2629..3948
                     /locus_tag="OO568_003383"
     CDS             2629..3948
                     /locus_tag="OO568_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6642610.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4234..4275
                     /locus_tag="OO568_003384"
     CDS             4234..4275
                     /locus_tag="OO568_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642611.1"
                     /translation="MVQLVDLARCVSF"
     gene            4313..5458
                     /gene="dosC"
                     /locus_tag="OO568_003385"
     CDS             4313..5458
                     /gene="dosC"
                     /locus_tag="OO568_003385"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6642612.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            5383..6000
                     /locus_tag="OO568_003386"
                     /pseudo
     CDS             5383..6000
                     /locus_tag="OO568_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            6258..6590
                     /gene="ddpX"
                     /locus_tag="OO568_003387"
                     /pseudo
     CDS             6258..6590
                     /gene="ddpX"
                     /locus_tag="OO568_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            6604..7500
                     /locus_tag="OO568_003388"
     CDS             6604..7500
                     /locus_tag="OO568_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6642613.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            7497..8483
                     /locus_tag="OO568_003389"
     CDS             7497..8483
                     /locus_tag="OO568_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6642614.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            8476..9402
                     /locus_tag="OO568_003390"
                     /pseudo
     CDS             8476..9402
                     /locus_tag="OO568_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(9458..9889)
                     /gene="osmC"
                     /locus_tag="OO568_003391"
     CDS             complement(9458..9889)
                     /gene="osmC"
                     /locus_tag="OO568_003391"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6642615.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2421 a         2483 c         2545 g         2593 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc tttctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtacc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca tacctgataa cgtttaacgg taacggtgtc ccgaaacgaa
      841 cccgtttcgg gataatttcc aaagtctgtt cactggcatt agcaacggaa aatattgttc
      901 tgaatacgct tcagaacaaa acaggtgcgg ttccgacagg aataccgttt tagggggata
      961 atatggctac atcagtacag acaggtaaag ctaagcagct cacattactc ggattctttg
     1021 ccataacggc atcgatggta atggctgttt atgaataccc taccttcgca acatcgggct
     1081 tttcattagt cttcttcctg ctattaggcg ggattttatg gtttattccc gtgggacttt
     1141 gtgctgcgga aatggccacc gtcgacggct gggaagaagg tggtgtcttc gcctgggtat
     1201 caaatactct gggtccgaga tggggatttg cagcgatctc atttggctat ctgcaaatcg
     1261 ccattggttt tattccgatg ctctatttcg tgttaggggc actctcctac atcctgaaat
     1321 ggccagcgct gaatgaagac cccattacca aaactattgc agcactcatc attctttggg
     1381 cgctggcatt aacgcagttt ggtggcacga aatacacggc gcgaattgct aaagttggct
     1441 tcttcgccgg tatcctgtta cctgcattta ttttgatcgc attagcggct atttacctgc
     1501 actccggtgc ccccgttgct atcgaaatgg attcgaagac cttcttccct gacttctcta
     1561 aagtgggcac actggttgta tttgttgcct tcattttgag ttatatgggc gtagaagctt
     1621 ccgcaactca cgtcaatgaa atgagcaacc ccgggcgcga ctatccactg gctatgttac
     1681 tgctgatggt ggcggcaatc tgcttaagct ctgttggcgg tttgtctatt gcgatggtca
     1741 ttccgggtaa tgaaatcaac ctctccgcag gggtaatgca aacctttacc gttctgatgt
     1801 cccatgtggc accggaaatt gagtggacgg ttcgcgtgat ctccgcactg ctgttgctgg
     1861 gtgttctggc ggaaatcgcc tcctggattg ttggtccttc tcgcgggatg tatgtcacag
     1921 cgcagaaaaa cctgctgcca gcggcattcg ctaaaatgaa caaaaatggc gtaccggtaa
     1981 cgctggtcat ttcgcagctg gtgattactt ctatcgcgtt gatcatcctc accaataccg
     2041 gtggcggtaa caacatgtcc ttcctgatcg cactggcgct gacggtggtg atttatctgt
     2101 gtgcttattt catgctgttt attggctaca ttgtgttggt tcttaaacat cctgacttaa
     2161 aacgcacatt taatatccct ggtggtaaag gggtgaaact ggtcgtggca attgtcggtc
     2221 tgctgacttc aattatggcg tttattgttt ccttcctgcc gccggataac atccagggtg
     2281 attctaccga tatgtatgtt gaattactgg ttgttagttt cctggtggta cttgccctgc
     2341 cctttattct ctatgctgtt catgatcgta aaggcaaagc gaataccggc gtcactctgg
     2401 agccaatcaa cagtcagaac gcaccaaaag gtcacttctt cctgcacccg cgtgcacgtt
     2461 caccacacta tattgtgatg aatgacaaga aacactaacc atgtctgaat ggttataaga
     2521 caagggagcg ataattcatc gctccctttt tcgtgcttgc tgtcgtattg acttaaccgg
     2581 gataagtacg agaatgagcg cacatctgtt taccggaaac cagcacatat ggatatctgc
     2641 tcccgaaaca agaaattaac gattagaaga ccagcgatac tggttgcatt ggcactttta
     2701 ctgtgtagtt gtaaaagcac gcctccagag tccatggtga caccaccagc aggttcaaag
     2761 ccaccagcca cgacgcaaca atcgtcacaa ccgatgcgtg gcatctggct ggccacggtt
     2821 tctcgcctcg actggccacc ggtttcctcg gttaacatta gtaatcccac cagccgagcc
     2881 cgtgtacaac aacaggcgat gatcgacaaa ctggatcatc tccaacgtct cggcataaac
     2941 acggtctttt tccaggtcaa gccggacggt accgccctgt ggccatcgaa aattttgccg
     3001 tggtccgatc ttatgaccgg taagattggt gaaaatccgg gttacgatcc gctgcaattc
     3061 atgctcgacg aagcccacaa gcgtgggatg aaagtacacg cctggtttaa cccctatcgc
     3121 gtatcggtta atacgaagcc cggtactatc agggaactga atagcactct gtctcaacaa
     3181 ccggcgagcg tctatgtgca acaccgtgac tggatcagaa cgtctggcga tcgctttgtc
     3241 ctcgacccgg gcatccctga ggttcaggac tggatcacat caatagtcgc tgaagtggtt
     3301 tcccgctatc cggtagatgg cgtgcagttt gacgactatt tctatactga atcaccgggt
     3361 tcacggctaa atgataacga aacgtaccgt aaatacggag gcgcatttgc gtcaaaagca
     3421 gactggcggc gcaacaatac tcagcagtta attgcaaagg tatcacacac cattaaaagc
     3481 attaagccgg gagtcgaatt tggcgttagc ccggcaggcg tgtggcgtaa ccgatcacac
     3541 gatccgctcg gttccgatac ccgaggcgcg gcagcctatg acgaatccta cgctgacacc
     3601 cgtcgatggg tggaacaagg attgctggat tacattgctc cccaaattta ctggccgttc
     3661 tcacggagtg ccgcgcgtta tgacgtgttg gcaaaatggt gggcggatgt cgttaaaccg
     3721 accaggaccc gcctgtatat cggtatcgcc ttctataaag tgggtgaacc ttcaaagata
     3781 gagccagact ggatgattaa cggcggcgta ccggaactga aaaagcagct cgatcttaac
     3841 gatgcggtac cagaaattag cggcaccatc ttgttccgtg aggactatct gaataaaccg
     3901 cagactcaac aagcggtcag ctatctgcaa agtcgttggg gcagttaaac taccatgctt
     3961 actttgtaac aagccggtgc tttacccacc ggcttgttac accttgtgaa atatcccact
     4021 agtaatcatg cttacataag tcaaattaac cacacttagt taaacagcta taaatcccta
     4081 ataaaaatgt atgaatatga aaaaccatac gatattcgct gtcaccgaaa aataattacg
     4141 atgggctata ctcttctgaa gaacagtagc gtagattgaa actgtcttaa gtcgcttatt
     4201 tcttttcagt atatcttcat atttcaggag aatatggtgc agttagtcga tctcgcacgt
     4261 tgtgtttcgt tttgattccg tctgatttcc cagccttata agggtgtgaa ttatggagat
     4321 gtattttaaa agaatgaaag atgagtggac cggacttgtc gaacaggcag atcctctcat
     4381 tcgtgctaaa gccgcagaaa ttgcccttgc gcatgctcat tatctgagta tcgagtttta
     4441 tcgaattgtc cgcatcgacc cgcatgccga agaattcttg agtaatgaac aagttgagcg
     4501 gcagttgaaa agtgcgatgg aacgctggat tattaacgtg ctttctgccc aggttgacga
     4561 tgtcgaaagg ctaatacaaa tccagcatac cgtcgcggaa gtgcatgccc gcataggaat
     4621 tccggtagaa attgtcgaga tggggtttcg ggtgctgaaa aaaatcctct atccggtaat
     4681 cttctcttcg gattattccg ccgcagaaaa acttcaggtc taccatttct cgattaacag
     4741 tattgatatc gcgatggaag tgatgacccg cgcgtttacc tttagtgaca gtagtgcctc
     4801 aaaggaagat gaaaactatc gtatcttctc gttactggaa aacgccgaag aagaaaaaga
     4861 acggcaaata gcctcaatac tttcatggga aatagatatt atctataaag tcctgctgga
     4921 ttctgattta ggcagcagtt tgcctttaag ccaggctgat tttggcctgt ggtttaacca
     4981 taaaggtcga cattatttta gcggcattgc cgaagtgggc catatctccc gtctgattca
     5041 ggatttcgac ggtattttca atcaaaccat gcgtaacacc aggattttga ataacagaag
     5101 tttgcgggtg aaatttctat tacagataag aaataccgta tcgcagatta ttaccttgct
     5161 gcgtgaattg tttgaagaag tatcgcgcca cgaagtcggt atggatgtac tgacgaaatt
     5221 acttaaccgc cgtttcctgc cgactatctt caaacgcgaa attgcccatg ccaaccggac
     5281 cggtacaccg ctgtcagtgc tgattattga cgttgataaa ttcaaagaga tcaacgatac
     5341 gtggggccat aacactggcg atgaaattct gcgtaaagtc tcattcctct cgcagaagag
     5401 attggtgaaa tcgaaaatat tgggcgctgg gtcatcgcgg aagcttgccg tcagttagca
     5461 gaatggcgta gccagaatat tcatatcccg gcgttatccg tgaacttgtc ggcgctgcac
     5521 tttcgcagta atcagctgcc taatcaggtg tctgatgcaa tgcaagcctg gggtattgac
     5581 ggccaccagc tgacggtgga aatcacggaa agcatgatga tggaacacga taccgaaatc
     5641 tttaagcgca ttcagatcct gcgtgatatg ggcgtgggtt tatcggtaga tgattttggc
     5701 acgggctttt ccggattatc ccgcttagtc agtcttccgg taacggaaat caaaattgac
     5761 aaaagctttg tcgatcgttg tctgacggaa aaacgcatcc tggccttact tgaagccatt
     5821 accagcattg ggcaaagcct caatttaacc gtcgtggcgg aaggggtcga aaccaaagag
     5881 caatttgaga tgctacgtaa gatccactgt cgcgttattc agggatattt cttttcccgc
     5941 ccccttcccg ccgaagaaat tccaggctgg atgagcagcg tgttaccgct gaaaatctga
     6001 caaattcctc tcgcccgcac tcgcgggttt tcctttaagg tgacactgtc acttaaatac
     6061 tgtgatttca gctacgattc cgggagattc cttccttaac tttccctctt gcaccaaaac
     6121 tgcactacgc tgcacaaatg ttgatcaaaa gttaaaaaat ataactttaa gccagcattc
     6181 tggcgcttat cccggcatgg catgagatct gcataagcgg aaagcgcagc aatttttgtc
     6241 ttatacagga accgtttatg tcggatacca ccgaactggt tgatttagcc gtgatcttcc
     6301 ctgatctgga gatcgaattg aaatatgcct gcgttgataa catcacaggt aaagctattt
     6361 atcagcaagc gcgttgtctg ctacataaag atgcgattac cgcgctggcg aaaagtatca
     6421 gtatcgccca gctgtcaggc ttacaactgg tgatttacga tgcgtatcgc ccacaacaag
     6481 cacaggcgat gttgtggcaa gcctgcccag acccgcaata tgttgttgat gtgacggtcg
     6541 gttctaatca cagccgtggc acggcgctcg acctgacgct tcgtgatgac gtggaggtgg
     6601 tgaatgatgc taagcgagga aacgtccgcc gtacgcccac aaaaacaaac gcgatttaac
     6661 ggtgcaaaac tggtatggat gctgaaaggc agtccgctca ccgtgaccgg cgcagtcatc
     6721 attgtattaa tgcgactgat gatgattttt tcaccgtggc tggcgacgca tgatcccaac
     6781 gccattgatt taaccgcccg ccttttgccg ccttctgcgg cgcactggtt tggcaccgat
     6841 gaagtgggac gcgatctgtt tagccgcgta ctggtcggca gtcagcaatc aattctcgcc
     6901 ggattagtgg tggtcgccat tgcgggtatg attggttcgc tactcggatg tctatccggt
     6961 gtgcttggcg gacgcgcaga cgccatcatc atgcgcatca tggacattat gctgtcgatt
     7021 ccttcgttgg tactgacaat ggcactggct gccgctctcg ggccgagttt atttaacgcc
     7081 atgctggcga ttgctattgt gcgaattccc ttttatgtgc gcctggcgcg ggggcaagca
     7141 ttagttgtac gccagtatac ctatgttcag gcggcgaaaa cctttggcgc gtctcgttgg
     7201 catctgatca actggcatat tttacgtaac tccctaccgc cgctgatcgt gcaggcatcg
     7261 ctggatatcg gtagcgcgat tttaatggcc gccacgttgg gatttattgg cctgggtgct
     7321 caacaaccga gtgctgaatg gggcgcgatg gtagcgaatg gtcgcaacta tgtactcgat
     7381 cagtggtggt attgcgcatt tccgggggca gcgattttgc ttaccgccgt cgggtttaat
     7441 ctctttggtg atggtattcg cgatctgctt gacccgaaag caggaggaaa gcagtcatga
     7501 cccaacccgt tctggacatt caacagctgc atttgagttt ccccggtttt aacggtgatg
     7561 ttcacgcgct caacaatgtg tccttgcaga ttaaccgcgg cgaaattgtc ggtctggtgg
     7621 gagaatccgg ctcaggtaaa tcagtcaccg caatgctgat tatgcgtctg ctaccgacgg
     7681 gcagttattg cgtacatcgg gggcagattt cactgctggg agaagatgtt cttaacgctc
     7741 gggaaaaaca gcttcgtcag tggcgcggcg cacgagtggc gatgatcttt caggaaccga
     7801 tgaccgccct taatccgaca cgtcgaatag gtcttcagat gatggacgta atccgccatc
     7861 atcaaccaat aagtcgtcgg gaagccagag ctaaagcgat tgccctgctg gaagagatgc
     7921 aaatcccgga tgccgtggaa gttatgtcgc gctatccgtt tgagctttca ggtggtatgc
     7981 gccagcgggt aatgattgcg ctggcattct cctgcgagcc gcaattgatt attgccgacg
     8041 aaccgacgac ggcgctggac gtcacagtac agttgcaggt actgcgtttg cttaaacata
     8101 aagcacgcgc cagtggaact gcggtactgt tcatcagcca tgatatggcc gtggtgtcgc
     8161 aactgtgcga tagcgtttac gtgatgtatg ccggaagcgt aattgaaagc ggcgggacgg
     8221 cagacgttat ccatcatccc cggcatccgt ataccattgg tttgctgcaa tgcgcaccag
     8281 aacatggagt accacgccag ccattacccg ccattccagg gacggtacca aacctcaccc
     8341 atttgcctga cggctgcgct tttcgcgatc gttgctatgc ggcaggtgca cagtgtgaaa
     8401 acgtcccggc gctgacagcg tgtggtgaca acaaccagca ctgcgcctgt tggtatccgc
     8461 agcaggaggt cataagtgtc tgacacgtta ttaacgttac gcgacgtcca tatcaatttt
     8521 ccggcccgta aaaactggct tggtaaatct acggaacatg ttcatgccat taatggtatt
     8581 gatttacaga tccgccgtgg tgaaacctta gggatcgtcg gcgagtcagg ctgcggcaaa
     8641 agcaccctcg cacagctttt aatgggcatg ctgcaaccga gccacgggca gtacatccgt
     8701 tcaggctcac aacgcattat gcagatggtg tttcaggacc cgctctcttc gcttaatccg
     8761 cgcttaccgg tgtggcgcat catcacagaa tcgctctgga tagctaagcg tagtagtgaa
     8821 caacagcggc gagcgttggc agaggagttg gctgtgcagg tgggtattcg tccggagtat
     8881 ctcgaccgcc tgcctcatgc gttctccggc gggcagcggc aacgcatcgc cattgccagg
     8941 gcactctctt cgcagcctga cgtgattgtg cttgatgagc caacctcggc gctggatatc
     9001 tccgtgcagg cgcagatcct caatttactg gtaacgctac aggaaaatca cgggctgact
     9061 tatgtgctga tttcacacaa tgtctcggtg atacgtcata tgagcgatcg ggtggcggtg
     9121 atgtatctcg ggcagattgt agaactgggc gacgcgcaac aggtgttgac tgcttcggca
     9181 catccataca cccgattatt gctggattcc ctccccgcca ttgataaacc gctggaggaa
     9241 gaatgagcat tacgtaaaac ggagctgccg ggaaaccgca cgttgccgca aggctgtttt
     9301 ttctacgaac gttgccctct ggcaacccac ggatgtgaag tccggcaatc attagcaata
     9361 agggaggacg gacgtgagct ccggtgctgg cgggcgctgt agagatgcta acaacgcatc
     9421 aggcattcac gcgcctgacg cgtcatccgg caatgcttta cgatttcaac tggtaatcca
     9481 gcgtaatttc cgctttcagt acctgagaga ccgggcatcc tgcttttgct ttctggatga
     9541 tgccgtcaaa ggtagaggca tcaatacccg gcaccgcaac ttcactcttc agtgcgattt
     9601 tcgtaatcgc aaaaccggca tccactttat ccagcgacac atcggcggtg gtatcaattg
     9661 atgttggcgt gaatcccgct tcccccagca ttaatgaaag cgccattgag aaacatgcgg
     9721 catgcgctgc gccaatcagt tcttcagggt tggttccttt ttcgccttca aaacgcgtgt
     9781 taaatccata cggctgttgg ttcagcacgc cgctctcggt ggatactgtt cccttcccgc
     9841 gtttgatatc gccttcccag tgtgcctgac ctttcttatg gattgtcatt gttgctctcc
     9901 tgtgggctta aaaaggaaat tatagactac gtgagaaaca gcacgaggat aagcaggata
     9961 ttccgaataa aatacggcga aaatattact tctgcactta aattaaattc ccacaataga
    10021 taaccgggaa tatatatctc aa
//
LOCUS       DAJTYG010000119         9947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTYG010000119 DAJTYG010000000
VERSION     DAJTYG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.119"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OO568_003392"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OO568_003392"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6642616.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OO568_003393"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OO568_003393"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6642617.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3326)
                     /locus_tag="OO568_003394"
                     /pseudo
     CDS             complement(2698..3326)
                     /locus_tag="OO568_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3456..5216
                     /locus_tag="OO568_003395"
     CDS             3456..5216
                     /locus_tag="OO568_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6642618.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5622..6479
                     /gene="focA"
                     /locus_tag="OO568_003396"
     CDS             5622..6479
                     /gene="focA"
                     /locus_tag="OO568_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6642619.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6534..8816
                     /gene="pflB"
                     /locus_tag="OO568_003397"
     CDS             6534..8816
                     /gene="pflB"
                     /locus_tag="OO568_003397"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6642620.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9008..9748
                     /gene="pflA"
                     /locus_tag="OO568_003398"
     CDS             9008..9748
                     /gene="pflA"
                     /locus_tag="OO568_003398"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6642621.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2548 a         2493 c         2375 g         2531 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcttggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aatgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccatta aagaactcaa
     3001 attccgtcat gttggagtta ttgagtttcg accaggccag ctcgccatca cattaccaag
     3061 cggtataaca acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca
     3121 taaaacgcca catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc
     3181 gacatctgtc gcacaccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag
     3241 gtataaagac tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc
     3301 atacggtgga gatcgaacgc cttcattttt atgctcttaa cgtggtttat ccgctaaaag
     3361 catagtcaac agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat
     3421 tattgcgcgc tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca
     3481 aagatgccgc tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct
     3541 ttcagattga agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc
     3601 gcgacaaaga gtgcgcactg tgttttacca acggtaaagg cgcaaccaag aaagcggcgc
     3661 tggcttctgc actcggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt
     3721 tctggctggg cgaaaccatc gccaacggtc catttgtgca ttatcccaac gaaaaatggt
     3781 tcccactgag cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcat
     3841 tttacgatcc ggagaatgaa ttgaccggca gtatgctgat tgacctacaa tccggtaacg
     3901 aagatcgtgg tatttgcggt ctgccgttta cgcgccagtc cgacaatcag accgtttata
     3961 ttccgatgaa tatcattggt aacctgtacg tttctaacgg tatgtccgct ggcaataccc
     4021 gcaacgaagc acgcgttcag gggttgtccg aagttttcga acgctacgtg aaaaaccgca
     4081 ttattgctga aagcatcagc ttgccggaaa tcccggcaga cgtgctggcg cgttacccag
     4141 cggtggttga agcgatcgaa acactggaaa cagaaggttt cccgatcttc gcatatgatg
     4201 gttcccttgg cggccagtat ccggtgattt gcgtggtact gtttaatcct gcaaacggca
     4261 cctgctttgc ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga
     4321 ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg
     4381 atgatgaaga agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt
     4441 taatctcctg ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct
     4501 ccggcaccac ggaagaagag tttgctacgc tgatggctat cttcaacaaa gaagataaag
     4561 aagtttatat tgccgattac gagcatctgg gcgtttatgc ttgccgtatt atcgtgcctg
     4621 gcatgtccga tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc
     4681 atttacgtga aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga
     4741 acctcatcga gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt
     4801 tgggtctggc gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag
     4861 ccatgctggc gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga
     4921 tggagtttaa ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa
     4981 cgttgttatt actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg
     5041 ttcgcatgta cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg
     5101 cgttttacgg cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt
     5161 tgttgaaggc ctacgaaaag ctgcagcgcg ccaaagcagc attctgggca aaataaaatc
     5221 aaatagccta cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgtttttt
     5281 taatgccccg ctttacatat atttgcatta ataaaataat tgtaattata aggttaaata
     5341 tcggtaattt gtatttaata aatacgatcg atattgttac tttattcgcc tgatgctccc
     5401 ttttaattaa ctgttttagc ggaggatgcg gaaaaaattc aactcatttg ttaattttta
     5461 aaatttattt ttatttggat aatcaaatag ttactccgta tttgcataaa aaccatgcga
     5521 gttacgggcc tataagccag gcgagatatg atctatatca atttctcatc tataatgctt
     5581 tgttagtatc tcgtcgccga cttaataaag agagagttag tgtgaaagct gacaaccctt
     5641 ttgatctttt acttcctgct gcaatggcca aagtggccga agaggcgggt gtctataaag
     5701 caacgaaaca tccgcttaag actttctatc tggcgattac cgccggtgtt ttcatctcaa
     5761 tcgcattcgt cttctatatc acagcaacca ctggcacagg cacaatgccc ttcggcatgg
     5821 caaaactggt tggcggcatt tgcttctctc tggggctgat tctttgtgtt gtctgcggag
     5881 ccgatctctt tacttccaca gtgttgattg ttgttgctaa ggcgagtggg cgcatcacct
     5941 ggggtcagtt ggctaaaaac tggctaaatg tctattttgg caacctggtc ggcgcactgc
     6001 tgtttgtact tttaatgtgg ctttccggcg agtatatgac cgcaaatggt caatggggac
     6061 taaacgtcct acaaaccgcc gaccacaaag tgcaccatac ttttattgag gccgtctgtc
     6121 ttggtatcct ggcaaacctg atggtatgtc tggcagtatg gatgagttat tctggccgca
     6181 gcctgatgga caaagcgttc attatggtgc tgccggtcgc gatgtttgtt gccagcggtt
     6241 ttgagcacag tatcgcaaac atgtttatga tcccgatggg tattgtaatc cgcgacttcg
     6301 catctccgga attctggacc gctgtcggtt ctgcaccgga aaatttttct cacctgaccg
     6361 tgatgaactt catcactgat aacctgatcc cggttacgat cggtaatatt atcggcggtg
     6421 gtttgttggt tgggttgaca tactgggtca tttacctgcg tgaaaacgat caccattaat
     6481 ggttgtcgaa gtacgcagta aataaaaaat ccacttaaga aggtaggtgt tacatgtccg
     6541 agcttaatga aaagttagcc acagcctggg aaggttttac caaaggtgac tggcagaatg
     6601 aagtaaacgt ccgtgacttc attcagaaaa actacactcc gtacgagggt gacgagtcct
     6661 tcctggctgg cgctactgaa gcgaccacca ccctgtggga caaagtaatg gaaggcgtta
     6721 aactggaaaa ccgcactcac gcgccagttg attttgacac cgctgttgct tccaccatca
     6781 cctctcacga cgctggctac atcaataaag cgttggaaaa agttgttggt ctgcagactg
     6841 aagctccgct gaaacgtgct cttatcccgt tcggtggtat caaaatgatc gaaggttcct
     6901 gcaaagcgta caaccgcgaa ctggacccga tgatcaaaaa aatcttcact gaataccgta
     6961 aaactcacaa ccagggcgtg ttcgacgttt acactccgga catcctgcgt tgccgtaaat
     7021 ccggtgttct gaccggtctg ccagatgctt atggccgtgg ccgtatcatc ggtgactacc
     7081 gtcgcgttgc gctgtacggt atcgactacc tgatgaaaga caaatacgct cagttcacct
     7141 ctctgcaggc tgatctggaa aacggcgtaa acctggaaca gactatccgt ctgcgcgaag
     7201 aaatcgctga acagcaccgc gctctgggtc agatgaaaga aatggctgcg aaatacggct
     7261 acgacatctc tggtccggct accaacgctc aggaagctat ccagtggact tacttcggct
     7321 acctggctgc tgttaagtct cagaacggtg ctgcaatgtc cttcggtcgt acctccacct
     7381 tcctggatgt gtacatcgaa cgtgacctga aagctggcaa gatcaccgaa caagaagcgc
     7441 aggaaatggt tgaccacctg gtcatgaaac tgcgtatggt tcgcttcctg cgtactccgg
     7501 aatacgatga actgttctct ggcgacccaa tctgggcaac cgaatctatc ggtggtatgg
     7561 gcctcgatgg tcgtaccctg gttaccaaaa acagcttccg tttcctgaac accctgtaca
     7621 ctatgggtcc gtctccggaa ccgaacatga ccattctgtg gtctgaaaaa ctgccgctga
     7681 acttcaagaa attcgccgct aaagtgtcca tcgacacctc ttctctgcag tatgagaacg
     7741 atgacctgat gcgtccggac ttcaacaacg atgactacgc tatcgcttgc tgcgtaagcc
     7801 cgatgatcgt tggtaaacaa atgcagttct tcggtgcgcg tgcaaacctg gcgaaaacca
     7861 tgctgtacgc aatcaacggc ggcgttgacg aaaaactgaa aatgcaggtt ggtccgaagt
     7921 ctgaaccgat caaaggcgat ctcctgagct acgatgaagt gatggagcgc atggatcact
     7981 tcatggactg gctggctaaa cagtacatca ctgcactgaa catcatccac tacatgcacg
     8041 acaagtacag ctacgaagcc tctctgatgg cgctgcacga ccgtgacgtt atccgcacca
     8101 tggcgtgtgg tatcgctggt ctgtccgttg ctgctgactc cctgtctgca atcaaatatg
     8161 cgaaagttaa accgattcgt gacgaagacg gtctggctat cgacttcgaa atcgaaggcg
     8221 aatacccgca gtttggtaac aatgatccgc gtgtagatga cctggctgtt gacctggtag
     8281 aacgtttcat gaagaaaatt cagaaactgc acacctaccg tgacgctatc ccgactcagt
     8341 ctgttctgac catcacttct aacgttgtgt atggtaagaa aactggtaac accccagacg
     8401 gtcgtcgtgc tggcgcgccg ttcggaccgg gtgctaaccc gatgcacggt cgtgaccaga
     8461 aaggtgctgt agcgtctctg acttccgttg ctaaactgcc gtttgcttac gctaaagatg
     8521 gtatctccta caccttctct atcgttccga acgcactggg taaagacgac gaagttcgta
     8581 agaccaacct ggctggtctg atggatggtt acttccacca cgaagcatcc atcgaaggtg
     8641 gtcagcacct gaacgttaac gtgatgaacc gtgaaatgct gctcgacgcg atggaaaacc
     8701 cggaaaaata tccgcagctg accatccgtg tatctggcta cgcagtacgt ttcaactcgc
     8761 tgactaaaga acagcagcag gacgttatta ctcgtacctt cactcaatct atgtaattag
     8821 atttgactga aatcgtacag taaaaagcgt acaataaagg ctccacgaaa gtggggcctt
     8881 ttttagcacg agagcctttt ttgtcagcta tctatacttt aaggtgactg ccaaaacaga
     8941 ctcgacgtag ccttcgagct gcgcaccaac acggcctcag atgggccaca tctggagaaa
     9001 caccgcaatg tcagttattg gtcgcattca ctcctttgaa tcctgtggaa ccgtagacgg
     9061 cccaggtatt cgctttatca cctttttcca gggctgcctg atgcgctgcc tgtattgtca
     9121 taaccgcgac acctgggaca cgcatggcgg taaagaagtt accgttgaag atttgatgaa
     9181 ggaagtggtg acctatcgcc actttatgaa cgcttccggc ggcggcgtta ccgcatccgg
     9241 cggtgaagca atcctgcaag ctgagtttgt tcgtgactgg ttccgcgcct gtaaaaaaga
     9301 aggcattcat acctgtctgg ataccaacgg ttttgttcgt cgttacgatc cggtgattga
     9361 tgaactgctg gaagtaaccg acctggtaat gctcgatctc aaacagatga acgacgagat
     9421 ccaccaaaat ctggttggag tttccaacca ccgcacgctg gagttcgcta aatatctggc
     9481 gaacaaaaat gtgaaggtgt ggatccgcta cgttgttgtc ccaggctggt ctgacgatga
     9541 cgattcggcg catcgcctcg gtgaatttac ccgtgatatg ggtaacgttg agaaaatcga
     9601 acttctcccc taccacgaac tgggcaaaca caaatgggtg gcgatgggtg aagaatacaa
     9661 actcgatggt gttaaaccac cgaagaaaga gaccatggaa cgcgtgaaag gcattcttga
     9721 gcagtacggt cataaggtaa tgttctaatt tccccgtaaa gcggcaactt tattgagttg
     9781 ccgctttttt attccgcgct tcattctgct acagcgtttt taacacatct tatagtgcat
     9841 ctaccatatc ccgtacctga gataataacg actgataata gctcgcgtga agttcttccc
     9901 catcgaatac cactgtatta cacaagaaag tgacatcggc atcttca
//
LOCUS       DAJTYG010000120         9659 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTYG010000120 DAJTYG010000000
VERSION     DAJTYG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.120"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            373..1491
                     /locus_tag="OO568_003399"
     CDS             373..1491
                     /locus_tag="OO568_003399"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6642622.1"
                     /translation="MPLPDFHFSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1557..1805
                     /locus_tag="OO568_003400"
     CDS             1557..1805
                     /locus_tag="OO568_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR6642623.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1870..2238
                     /locus_tag="OO568_003401"
     CDS             1870..2238
                     /locus_tag="OO568_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6642624.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2332..2985
                     /gene="nfsB"
                     /locus_tag="OO568_003402"
     CDS             2332..2985
                     /gene="nfsB"
                     /locus_tag="OO568_003402"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6642625.1"
                     /translation="MNIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3093..4340
                     /gene="ybdG"
                     /locus_tag="OO568_003403"
     CDS             3093..4340
                     /gene="ybdG"
                     /locus_tag="OO568_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6642626.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTKAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4408..5784)
                     /gene="pheP"
                     /locus_tag="OO568_003404"
     CDS             complement(4408..5784)
                     /gene="pheP"
                     /locus_tag="OO568_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6642627.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGDAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGRNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMTLVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5885..9020)
                     /gene="cusA"
                     /locus_tag="OO568_003405"
                     /pseudo
     CDS             complement(5885..9020)
                     /gene="cusA"
                     /locus_tag="OO568_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(9032..9573)
                     /locus_tag="OO568_003406"
                     /pseudo
     CDS             complement(9032..9573)
                     /locus_tag="OO568_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
BASE COUNT         2369 a         2680 c         2333 g         2277 t
ORIGIN      
        1 atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg gaaagcttca
       61 ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat acagcaaatc
      121 ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt tttttctgca
      181 tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg ccacattgaa
      241 aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa agaaacattc
      301 cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa aacgtcaact
      361 taagagaaac ctatgccatt acccgatttt catttttctg aaccttttac cctcggtatt
      421 gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga ctcttcaatg
      481 ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga tatcaccgaa
      541 agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc cgggcagttt
      601 tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga aatttgcggc
      661 ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa cgaacgctat
      721 caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt tggtcagcat
      781 gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg cttgtcacgg
      841 tttgtgccgc attttattgc cctttccgcc gcgtcgccat atatgcaggg aacggatacg
      901 cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg cccgatgccg
      961 tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta caccacgatg
     1021 atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt tggcacggtg
     1081 gaggttcggg tgatggatac cccgttaacc cttagccacg cggtgaatat ggcgggatta
     1141 attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca ggagaaagat
     1201 tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga aggcgtcatt
     1261 accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg attgctggaa
     1321 aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc actgcatcgc
     1381 caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc cgatggcggc
     1441 tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta agccgggaat
     1501 tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac aatgttatga
     1561 aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct ttcctctctt
     1621 gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg gtgaccatgt
     1681 tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg tttttagtgc
     1741 tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc tcgctggcgg
     1801 attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc ttcgcctaaa
     1861 ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc tcttgagtta
     1921 ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa aattggcggc
     1981 aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct gaagtccgat
     2041 ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg gtatcacatg
     2101 aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc gttacttcgc
     2161 gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg cgatcaaaaa
     2221 agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta ccatttctcg
     2281 ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt tatgaatatc
     2341 atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa aaaacttacc
     2401 ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag caccaactcc
     2461 cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt tgccaaatcc
     2521 gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca cgtcgtggtg
     2581 ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga ccaggaagat
     2641 gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg tcgcaagttc
     2701 ttcgccgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc aaaacaggtt
     2761 tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga cgcggtgccc
     2821 atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga gaaaggctac
     2881 accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa cgctacgctg
     2941 ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc tcttgccggg
     3001 catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt ttcagccgtc
     3061 atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc caggttgaag
     3121 atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc ggtattattt
     3181 ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg accttcgaaa
     3241 aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat aaactcttcc
     3301 accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta ttctggctgc
     3361 aaaaaggcac caaagcggca gatattctga ctacctgcgc gcagttgtgg atcatgatgt
     3421 atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg cagaaattcc
     3481 cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg atcggcgcaa
     3541 tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg attctgatca
     3601 gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg attcttggtc
     3661 tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac tggctggaaa
     3721 tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc gtcaaagtgc
     3781 gtaactggga caataccatt accactattc ccacctggtc tctggtttct gactccttta
     3841 aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt atcagtattg
     3901 atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat aaagcgcatt
     3961 tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat cgccagcaag
     4021 gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc tttcgtgcct
     4081 atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc ttaatggtac
     4141 gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt actaacaccg
     4201 tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata tttgcgattg
     4261 tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt cgctctctgg
     4321 cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga gagcggacat
     4381 cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa atgccacaaa
     4441 caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat ccatcgtgca
     4501 catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga acggatagag
     4561 cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa aacgcagatg
     4621 cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg tcatcagcag
     4681 accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag tgatcgctcc
     4741 ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca aaaacttcgg
     4801 cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa ccccgctgtt
     4861 atacaccgac agcgatgcca ccagaatgac gaagttcagc gcagaagcta ccacattgct
     4921 gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt ccacccacgg
     4981 atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggattc gatacaccac
     5041 ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg ctgtaatccc
     5101 aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa tcagcccatt
     5161 ccggccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg ctttctcgcc
     5221 gccgtgacca gaaaacagca gccacaggcc aaagccgatc ataccgatga tcgccagcac
     5281 tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca ccaggttaac
     5341 ggcgttgatg ataataaaga aggcagcagc ccaaatccac gttggaacat ccgggaacca
     5401 gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca cgaacattac
     5461 ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat aggcaaagtg
     5521 ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc gcataatcag
     5581 gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg ccatctgaat
     5641 cgccgggcca atgccaagaa acagaccagt accaattgcg tcacccagcg caatcagttg
     5701 aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg cagtatcttc
     5761 cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt tgaggggcac
     5821 cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa cgaggtatcc
     5881 tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc gccgggataa
     5941 taaacagcga cagtaaaggt gcggtgatca tgccgccaat catcggtgcg gcaatccggc
     6001 tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg gcgataatca
     6061 ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac aacgcctcat
     6121 ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc tctatggcgt
     6181 gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca agggcaataa
     6241 agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac cagatgccgc
     6301 ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc cgacggaatg
     6361 ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt ttcagcttat
     6421 ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg cccggtttta
     6481 actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc atgtcacgat
     6541 cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc agcatcgacg
     6601 gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc ttcatcggcg
     6661 tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc ggataacgca
     6721 gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc ccgcccaccg
     6781 cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg gctttttcac
     6841 ggttaatctc aacgttgata tagcgcccac cttccagccg ctcggcaagc gcagaagcta
     6901 cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg atatccgcca
     6961 gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag agcatatcga
     7021 tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc acggtgttat
     7081 ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc tgtggcttaa
     7141 gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt tccgctttcc
     7201 cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg tcggtttttt
     7261 gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc atatacaaca
     7321 agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc ggccagagaa
     7381 ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc agtactttca
     7441 gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg ctttccggcg
     7501 gaattttgcc acggatccag taacccatca ggatagggat cactacgatc gccagcagcg
     7561 ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga cgcccttcct
     7621 gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga ctgataaaca
     7681 gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt ttattatcca
     7741 gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgcgca ttctcgatca
     7801 tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc agcgacataa
     7861 tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac cccagcggca
     7921 acgaaataat cgccaccagc gccgagcgca catgccagag aaacagcgcg cagactaccg
     7981 ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc cggtcaatga
     8041 gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta cttttcagcg
     8101 tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg gatcgcagga
     8161 tcaccacccc gcccgccact tcgccttcgc cgtttaatgc cccggcgcat ctccgggcca
     8221 atctggtcct tcgcaacatc gcgcagataa acgggcacgc cattttcact ggcttttaaa
     8281 acgatgtgat taaagtcgtc gagcgtttgt agatagccgc tggcgcgcac catatattct
     8341 gcttccgcca gttcgattga cgaaccgccc gcttcctggt ttgaagcatc cagcgcgctt
     8401 tttacttcgg cgagactgat gccatactgc gccaggcgct gaggatcgat aaccacctga
     8461 tactctttca ccacaccgcc taccgacgcc acttccgcaa cgtcaggaat ggttttcagc
     8521 tcatatttga gaaaccagtc ctgcaatgag cgtaaatcgg caagatcgtg cttaccgctg
     8581 cgatccacca gtgcatattc atagatccag ccaacacccg tggcatctgg tcccagctcg
     8641 gcgctgactc ccgcaggcag cttaccctgt acctggttga ggtactccag cacccgcgaa
     8701 cgcgcccagt acggatcggt gccatcttcg aaaatgacat acacataaga gtcgccaaac
     8761 tgcgagaaac cacgcacagt cttcgcgcca ggcaccgaca acatggtggt ggttagcgga
     8821 taagtcacct gattttcaac gatttgcggt gcctggccgg gatagctggt tttaataatc
     8881 acctgcacat cggagagatc cggcaacgca tccactggcg tattaatgat ggtccaggtg
     8941 ccccagatgc tcagaaacaa cgcgcccatc agcaccaaaa aacggttcgc caccgagcga
     9001 cgaataatcc attcaatcat tggttattcc ctcaatgcgc atgggtagca ctttcagagc
     9061 gcatccgctc cagtgcgcca gaaatattgg cttcggaatc aatcaggaac aggccgccgg
     9121 aaaccacctt ttcaccttcc gccagaccag agcgtaatgc ggtgacgcct tgcgatgcct
     9181 ggaaaacagc aacgcgtttc ggtacaaagc gcccgtcggc atcaacggta atcacccgct
     9241 gttcgttgcc ggtatcaatc agcgcctcgt cggcgttgtc gacttccaga cgcagctgca
     9301 gcgtgcgggt cgcggcatcc acgccaggta gcagcgtcca tttgcggatg gtgagtgttt
     9361 tatccggtcg cgccggaacg gtgagggtaa actgcgaggc atctttcacc agccaggcga
     9421 tggactccgg gatcgcagca gtgacccaca ccgggtccat accctgaatt cgcgattacg
     9481 cgcaataccc ctgagaaccg ccatgaacaa gggtttcatg atttgtgatt ttagtttagt
     9541 acgcaatttg gtacacaaca ctattttcac ttcagcgggt agtcatcaaa ctcaccataa
     9601 cgagcatcgt tgatgatgta tgtgatcacc acaaatatca catctggact agttccgtc
//
LOCUS       DAJTYG010000121         9525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTYG010000121 DAJTYG010000000
VERSION     DAJTYG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.121"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(437..643)
                     /locus_tag="OO568_003407"
     CDS             complement(437..643)
                     /locus_tag="OO568_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6642628.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMFFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            956..1045
                     /gene="kdpF"
                     /locus_tag="OO568_003408"
     CDS             956..1045
                     /gene="kdpF"
                     /locus_tag="OO568_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6642629.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1045..2717
                     /gene="kdpA"
                     /locus_tag="OO568_003409"
                     /pseudo
     CDS             1045..2717
                     /gene="kdpA"
                     /locus_tag="OO568_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
     gene            2740..4787
                     /gene="kdpB"
                     /locus_tag="OO568_003410"
                     /pseudo
     CDS             2740..4787
                     /gene="kdpB"
                     /locus_tag="OO568_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4796..5368
                     /gene="kdpC"
                     /locus_tag="OO568_003411"
     CDS             4796..5368
                     /gene="kdpC"
                     /locus_tag="OO568_003411"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6642630.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5361..6344
                     /locus_tag="OO568_003412"
                     /pseudo
     CDS             5361..6344
                     /locus_tag="OO568_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            6344..8043
                     /locus_tag="OO568_003413"
                     /pseudo
     CDS             6344..8043
                     /locus_tag="OO568_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141249.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
     gene            8040..8717
                     /gene="kdpE"
                     /locus_tag="OO568_003414"
                     /pseudo
     CDS             8040..8717
                     /gene="kdpE"
                     /locus_tag="OO568_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8992..9096
                     /locus_tag="OO568_003415"
     CDS             8992..9096
                     /locus_tag="OO568_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6642631.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9407..>9525
                     /locus_tag="OO568_003416"
     CDS             9407..>9525
                     /locus_tag="OO568_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6642632.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSV"
BASE COUNT         2120 a         2394 c         2637 g         2374 t
ORIGIN      
        1 aacataacat cttattggat tagatatgtt aacaattaat tattcttaat atatcaataa
       61 agtatatatt actctcttta ctatctgttt ttattgcggt agcgatacta ataacaaaat
      121 tcggaattta tttagatgct ggaattagaa aaagggatga cggctttctc accttaccct
      181 gaagtttcac gaaagtgtgt aaggaagtat gcaatctaac tggagatttc tgatgatcta
      241 gtcgcttaga gcgacaatga aggtgaccgc atcctgggta gccctatgaa aggatactat
      301 tgaaacgtga tgctaaaaca gtgatgaaat gaataatacg atggaaacgt cttccatgtt
      361 aattatcatg gaagacgttg atgttgaaca cctaaaccag ccccctgata cgaggctggc
      421 ttaagcgcgc aggcaatcag taaaaatcac cggttgcctt ttcggcctga tccatccaca
      481 ccggtttatc gctggttttc gaccagacgc ggtgcaggta gctgtaaaag cgggcgcggt
      541 ctttccagaa aaacatgaaa ggcagcgcca gaacgcccgc tgcaaccgca tatgtacggc
      601 gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc tccccctttt
      661 gtatctaccc ggtgaatagc accggaaaaa tgaatttgtt tatctgatga aaatagtacc
      721 gccttttgtg taattttacc actcatccga ccacttattt ttgcttattg atggtttatt
      781 tacattcaca ctgtaattaa attacacaaa agttaaatta atactaacca tcagttaaat
      841 catggccttt gccattttta tacttttttt acaccccgtc cgcagatttt tgcgaaatct
      901 ttgcagccag aattctactc ttccggtatc acttttaggc tactggaggt gcactgtgag
      961 tgcaggcgtg atagccggcg tgttgctggt gtttttatta ctgggttatt tggtttatgc
     1021 cctgatcaat gcggaggcgt tctgatggct gcgcaagggt tcttactgat tgccacgttt
     1081 tactggtatt aatggtgctg gcgcgtcctt taggcagcgg gctggcgcgg ctgattaatg
     1141 acattcctct tccaggtaca gcgggcgttg aacgtatact ctttcgttta cctggcgtct
     1201 ctgaccatga gatgaactgg aagcaatatc tttgtgccat tctcggcctg aacatgctgg
     1261 ggctggcggt gctgtttttt atgttgctcg gtcagcacta tctgccgctt aatccacagc
     1321 agttgccggg gctgtcgtgg gatctggcgc tgaataccgc cgtcagcttt gtcacgaata
     1381 ccaactggca atcttatagc ggtgaaacca cgttgagcta tttcagccag atggcaggct
     1441 taacggtaca aaactttctt tctgccgcca gcgggattgc ggtgattttt gccttcatcc
     1501 gtgcgtttac tcgccagagc atgagcacac tcgggaatgc ctgggtcgat ctgctacgca
     1561 tcacgttatg ggtgctagtc cctgtggcgt tgttaatcgc actgtttttt attcaacaag
     1621 gtgcgctgca aaactttctg ccctatcagg cagtgaatac cgttgaagga gcgcaacagc
     1681 tgttacccat gggaccagta gcttctcagg aagcgatcaa gatgctcggt actaacggcg
     1741 gtggcttctt taatgccaac tcgtcgcatc cgtttgaaaa cccaaccgca ctgaccaact
     1801 tcgtgcagat gctggcgatc ttcttgatcc caacagcgct gtgctttgcc tttggtgaag
     1861 tgacgggcga tcgccgccag gggcgcatgt tgctgtgggc gatgtcagtg atttttgtca
     1921 tctgcgtagg cgtggttatg tgggcagaag ttcagggtaa tcctcatctg ctggcgctcg
     1981 gcgcagacag cagcatcaat atggaaggta aagagagccg tttcggcgtg ctggtcagta
     2041 gcctgtttgc ggtcgtgact acggcggcgt cctgtggcgc ggtgattgcg atgcatgatt
     2101 cgtttaccgc tctcggcggc atggtgccga tgtggctaat gcaaattggt gaagtggtgt
     2161 tcggcggtgt cggttctggt ctttacggca tgatgctgtt tgtcctgctg gcggtgttta
     2221 ttgccgggct gatgattggt cgtacaccgg aatatctggg taaaaaaatc gacgtacgcg
     2281 agatgaaact gactgcactg gcgattctgg ttactccaac actggtactg atgggcgcgg
     2341 cgttggcgat gatgaccgac gccggacgta gcgccatact taaccctagc ccgcatggtt
     2401 ttagcgaagt gctgtacgcc gtgtcgtccg ccgctaacaa caacggcagc gcctttgccg
     2461 gattaagcgc caactctccg ttctggaact gtttactggc gttctgcatg tttgtcggtc
     2521 gcttcggggt gattatcccg gtgatggcaa ttgccggttc gctggtgagt aaaaagagcc
     2581 aacccgccag ctccggcacg ctgccaacgc acggcccgct gtttgttggc ctgttaatcg
     2641 gcaccgtgtt gctggttggc gcactgacct ttatccctgc cctggcgctt ggtccggtgg
     2701 cggaatatct ctcctgatga tattgagtga gcactgaata tgagtcgtaa acaactggcg
     2761 ctattcgaac caacacttgt cgttcaggcg ctgaaagaag cggtgaaaaa attaaacccg
     2821 caggcgcaat ggcgcaatcc ggtgatgttt atcgtctgga tcggcagtct gctgaccacc
     2881 tgtattagca tcgcgatggc aagcggtgcg atgcctggca atgcgctgtt tagcgcggca
     2941 attagcggct ggctgtgggt caccgtactg ttcgctaatt tcgccgaggc gctggcagaa
     3001 ggccgcagta aagcgcaggc caacagtctt aaaggggtga aaaaaactgc ctttgaccgc
     3061 aagctgcgtg agccgaaata tggcgctgcg gcggacaaag ttcctgccga tcaactgcgt
     3121 aaaggtgaca tcgtgctggt agaagctggc gatattatcc cctgcgatgg tgaagtcatt
     3181 gaaggcggcg catcggtcga tgaaagcgcc atcaccgggg aatcggcacc ggtgatccgt
     3241 gaatccggcg gcgattttgc ctccgtcacc ggcggcacgc gtattctttc tgactggctg
     3301 gtgattgagt gtagcgttaa ccccggtgag acatttctgg atcggatgat cgcgatggtg
     3361 gaaggcgcac agcgacgcaa aacaccgaac gagattgccc tgactatcct gctgattgct
     3421 ctgaccatcg tctttttacc ggcaaccgcc acgctgtggc cgttttccgc gtggggcggt
     3481 aatgccgtga gtgtaacggt actggtggcg ctgctggtct gtctgatccc aaccactatt
     3541 ggtggcctgt tgtcagcgat cggcgtcgcc gggatgagtc ggatgctggg cgcgaatgtg
     3601 attgccacca gcggacgtgc agttgaagcg gcaggtgacg ttgacgttct gctactggat
     3661 aaaaccggca ccatcacact cggtaaccgt caggcgtcgg agtttatccc cgcgcagggc
     3721 gtggatgaaa aaacgctggc tgacgccgca caactggctt cgctggctga tgaaacgccg
     3781 gaaggtcgca gtattgtgat cctcgccaag caacgtttta acctgcgcga gcgcgatgtg
     3841 cagtcgctcc atgccacctt tgtaccgttt actgcgcaaa gccggatgag cgggatcaac
     3901 atcgacaacc gcatgatccg taaaggttct gtcgatgcca ttcgccgcca tattgaggct
     3961 aacggcggtc acttccctgc cgatgttgat caaaaagtcg atcaggttgc gcgtcaagga
     4021 gccacgccgc tggtggtggt ggaaggttct cgtgtgctgg gcgttattgc gctgaaagat
     4081 atcgtcaaag gcggtattaa agagcgcttc gcccagctgc gcaaatgggc attaaaacgg
     4141 tgatgattac cggcgataac cgtctgactg ccgccgcgat agctgcggaa gcgggtgtcg
     4201 atgattttct cgccgaagcg acaccggagg ccaagctggc attgatccgt cagtatcagg
     4261 cggaaggtcg tttggtagcg atgaccggcg acagcaccaa cgatgctccg gcgctggcgc
     4321 aggcagatgt cgcggtggcg atgaactccg gcacccaggc ggcgaaagag gcgggcaata
     4381 tggtggatct cgactctaac ccgaccaagt tgatcgaggt ggtgcacatt ggcaaacaga
     4441 tgctgatgac ccgtggctcg ctgaccacct tcagcattgc caacgatgtg gcgaaatact
     4501 tcgccattat tccggcggcg ttcgcggcaa catatccgca gttaaatgcg ctgaacatca
     4561 tgcgcctgca ttcgcccgac tccgccattc tcagtgcggt cattttcaac gccttgatta
     4621 tcgtcttttt gattcctctg gcgttaaaag gcgtgagtta taaaccgctt accgcttctg
     4681 ccatgttgcg ccgtaactta tggatttacg gtctgggtgg gctgctggtg ccgtttattg
     4741 gtatcaaagt cattgattta ctgctaaccg tttgtagtct ggtgtgaggt ttaccatgag
     4801 tggattacgt ccggcattat cgacatttct ctttctgtta ttgattactg gcggcgttta
     4861 cccgctgctg accaccgcac tggggcaatg gtggtatccc tggcaggcca acggttcgtt
     4921 gattcgtgaa ggtgatacgg tgcgcggttc agcattaatc gggcaaaatt ttaccggcaa
     4981 cggctatttt catggtcgcc cgtcggcaac agcagaaatg ccctataatc cacaggcttc
     5041 tggcgggagc aatctggcag tcagtaaccc ggagctggat aaacagatag ccgcacgcgt
     5101 tgccgcatta cgggccgcta acccggacgc cagcacgaac gttccggttg aactggtgac
     5161 ggcgtcggca agcggactgg acaataacat caccccgcaa gcggcggcct ggcagatccc
     5221 acgcatcgca aaagcgcgta atctcagcgt tgaacagctc acgcagctga tcgcaaaata
     5281 cagccaacaa ccgctggtga aatatatcgg ccagccggtc gtcaacattg tcgaactcaa
     5341 tctggcactg gataagcttg atgaataacg aacccttacg tcccgacccc gaccgtctgc
     5401 tggaacaaac tgctgcgccg catcggggga agctgaaagt tttcttcagt gcctgtgcag
     5461 gcgtcgggaa gacctgggcg atgctggcag aagcccagcg actgcgggcg caagggctgg
     5521 atattgtggt tggtgtggta gaaacccatg ggcggaaaga taccgccgcc atgcttgaag
     5581 ggctggctat tctgcctctg aaacgccagg cgtaccgtgg gcggcatatc agcgagtttg
     5641 atctcgatgc cgccctcgcc cgccgtccgg cgctgatctt aatggacgaa ctggcgcaca
     5701 gtaatgcgcc aggttctcgt catcctaaac gctggcagga tatcgaagaa ctgctggaag
     5761 ctggcattga tgttttcaca accgtcaacg ttcagcatct ggaaagtctg aatgatgtgg
     5821 tcagcggcgt caccggaatt caggtacggg aaaccgtgcc cgatcctttt ttcgatgccg
     5881 ccgacgacgt ggtgctggtg gacttgcccc cggacgatct gcgccagcgg ctgaaagaag
     5941 gcaaagtcta tattgccggg caggcggagc gcgccattga acattttttc cgcaaaggca
     6001 atctgatcgc cctgcgcgaa ctggcactgc gccgtactgc cgatcgcgtt gatgagcaaa
     6061 tgcgcgcctg gcgggggcat cctggcgaag agaaagtctg gcacacgcgc gacgcgatcc
     6121 ttttatgcat cggccataac accggcagcg aaaactggtc cgcgcggcgg cgcggctggc
     6181 gtcacggctg gggagcgtct ggcacgcggt gtatgttgaa ccccctgccc tgcatcgctt
     6241 accggagaaa aaacgtcggg caattctcag cgccttacgt ctggcgcagg agctgggcgc
     6301 ggagacggca acactttctg atccagcaga agagaaagcg gtagtgcgtt atgcccgtga
     6361 acataatctc ggcaagatta ttctcggtcg cccagcctcg cgccgctggt ggcgtcggga
     6421 aacatttgct gaccgactgg cgcgcatcgc ccccgatctc gatcaggtgc tggtcgcgct
     6481 tgatgaacca cccgcccgca cgattaacaa cgcgccggat agccgctcat ttaaagacaa
     6541 gtggcgggta cagattcagg gatgcgtggt tgccgccgcg ttatgcgccg ttatcacctt
     6601 aattgccatg cagtggctga tggcgtttga tgccgccaac ctggtgatgc tgtatctgct
     6661 tggcgtggtg gtggtggcgc tattttatgg acgctggcct tcggtggttg ccaccgtcat
     6721 taatgtggtg agtttcgatc tcttttttat cgccccacgc ggcacgctcg ccgtctctga
     6781 tgtgcaatat ctgctgacct tcgcggtgat gttaaccgtc gggctggtga tcgggaacct
     6841 tactgctggc gtgcgttatc aggcgcgggt agcccgttac cgcgagcaac gcacacggca
     6901 cttatatgaa atgtcgaaag ctctggcggt gggccgcagt ccgcaggaca tcgccgccac
     6961 cagcgaacaa tttattgcct ctacgtttca tgcccgcagt caggtgttgt tgcccgatga
     7021 caacggtaaa ttgcagccgt taacacatcc gcaaggaatg acgccatggg acgatgccat
     7081 cgcgcagtgg agttttgata aaggcctgcc tgcgggcgcg ggcaccgaca cgttacccgg
     7141 tgtaccgtac cagattttgc cgctaaaaag cggcgagaaa acctacggct ggtggtggtg
     7201 gagccgggga atctgcgcca gttgatgatc ccggaacagc agcgcctgct ggagactttt
     7261 acgctgttag tcgccaatgc ccttgagcgg ctgacgctaa ccgccagcga agaacaggcg
     7321 cggatggcaa gcgaacgtga acagatccgc aacgccctgc tggcagcgct ttcgcatgat
     7381 ttacgcacgc cgcttacggt gctgtttggt caggcagaaa tcttaacgct cgatctggca
     7441 agcgaaggat caccccacgc ccgccaggcc agcgagattc gtcagcatgt gctgaacact
     7501 acccgactgg tgaataatct actggatatg gcgcgaattc agtccggcgg ctttaatttg
     7561 aagaaagagt ggttaacgct ggaagaagta gtcggcagcg cgctgcaaat gctggaaccg
     7621 ggtttatcgt cgcccatcaa tctttctctg ccagaaccgc tgaccttaat ccacgttgac
     7681 gggccactct ttgaacgggt gctgattaat ctgctggaga acgcggtgaa atatgcgggt
     7741 gcgcaggccg aaattggtat cgatgcccac gttgagggcg aaaatctaca actggatgtc
     7801 tgggataacg gccccggtct tccgccaggc caggagcaga cgatatttga taagtttgct
     7861 cgcgggaata aagagtcggc agtgccgggt gtcgggcttg ggttggcaat ttgtcgggcg
     7921 attgtggatg tacacggcgg caccattacc gcgttcaacc gaccggaagg tggtgcctgt
     7981 tttcgtgtta cacttcccca gcaaaccgct cctgaacttg aagactttca tgaggatatg
     8041 tgacaaacgt tctgattgtt gaagatgaac aggctattcg tagctttctg cgcacggcgc
     8101 tggagggcga cgggatgcgc gtctttgagg ccgaaactct gcaacgcggc ttgctggaag
     8161 cggcaacccg taagccagat ttgattattc tcgatctcgg cctgcccgat ggtgatggga
     8221 ttgagtttat ccgcgacctg cgccagtgga gcccggtgcc ggtgattgtg ctttccgcac
     8281 gcagctaaga gagcgacaag atcgccgcgc tggatgccgg agcggatgat tatctgagta
     8341 agccgtttgg cattggcgaa ttgcaggccc gtctgcgcgt cgctttacgc cgccactccg
     8401 ccaccgccgc gcccgatccg ctggtgaaat tttccgatgt taccgtcgat ttagccgccc
     8461 gcgtgattca ccggggtgag gaagaggtgc atctcacacc aattgagttc cgcctgctag
     8521 cggtgctgct caacaatgcc ggaaaagtac tcacccagcg ccagctcctt aaccaggtgt
     8581 gggggccaaa cgcggtcgaa cacagtcact atttgcgtat ttatatggga catctgcgac
     8641 aaaaactgga acaggatccg gcccggcctc gccacttcat tactgaaacc ggtattgggt
     8701 atcggtttat gctttgaatt gttattcaat aaattcgctt aaatagtgat taatttatta
     8761 ttaaacctgc gccgaatatt attatttggc aggtttaatt tcttaacgca aatataaaac
     8821 acgaaattac aacatttaag taacaaaaca gcatctaata tggcgatatt cataattaat
     8881 gatttattat ctggggtatg atcgtttttt gttgatcttc ttcacagatt atagccattt
     8941 catggataga ataactctac cttcaactga cacagcaagc ggtaaaggta aatggaaaat
     9001 aataaccgct taatgcctca tataaggcgg acaacccata tcatgaagtt tgcccatcgt
     9061 aatagttttg actttcattt ctttaatgcc cgctagtctt ctgactaaag ggcaccccaa
     9121 cgtacaggtc tccctgactt taagcattac aggttaatac ctgtattcct cggtgctcat
     9181 atactgctaa cccttttaaa ctctaaataa ttcgagtcgc agcacttgca acttgaggta
     9241 tgacgagtat agccagttac cgggctggtc tgggttattg catctgcaaa aagcaaacta
     9301 ctgatttatt tatcagcggt ggagctttgc tttttttccg gcgtgatcga tttctccttt
     9361 gagaaattga ggacctgcta ttacctgaaa taaagagatg aataaaatgt cagaattaaa
     9421 aattgccgtt agccgttctt gcccggattg tttttccact catcgtgcat gcgtgaatat
     9481 tgacgaaagt aattatattg acgttgccgc cattatttta tcagt
//
LOCUS       DAJTYG010000122         9339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTYG010000122 DAJTYG010000000
VERSION     DAJTYG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.122"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..1598
                     /locus_tag="OO568_003417"
     CDS             <1..1598
                     /locus_tag="OO568_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HCR6642633.1"
                     /translation="NNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGGAI
                     FAKENSTLNLTDVIFSGNVAGGYSGAIYSSGTNDTGAVDLRVTNAMFRNNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSVRAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
     gene            1611..3542
                     /locus_tag="OO568_003418"
     CDS             1611..3542
                     /locus_tag="OO568_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6642634.1"
                     /translation="MEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMAD
                     SSGQHQDKGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDG
                     ATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDG
                     VTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTL
                     LFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQN
                     NAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPT
                     PDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYT
                     AAGQLAQQEDTSTVLLSGGLFSGCWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADNQ
                     NHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLEA
                     GYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAV
                     GVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAWQ
                     KGSDDFAQTAGFLSMTVKW"
     gene            complement(3670..4392)
                     /locus_tag="OO568_003419"
     CDS             complement(3670..4392)
                     /locus_tag="OO568_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6642635.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            4539..7166
                     /gene="gyrA"
                     /locus_tag="OO568_003420"
     CDS             4539..7166
                     /gene="gyrA"
                     /locus_tag="OO568_003420"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6642636.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7315..9003
                     /locus_tag="OO568_003421"
     CDS             7315..9003
                     /locus_tag="OO568_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCR6642637.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            9000..>9339
                     /locus_tag="OO568_003422"
     CDS             9000..>9339
                     /locus_tag="OO568_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HCR6642638.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYV"
BASE COUNT         2298 a         2275 c         2614 g         2152 t
ORIGIN      
        1 ccaataatgc cagcggtggg gccgtatttt tgcaacaagg agcggaattt tcactattac
       61 cagaaaatga aactggaatg actctgtttg ccaataacac cgttacagga gaatataata
      121 acggcggggc catatttgct aaagaaaact caacgctgaa tcttactgat gttatttttt
      181 ccggtaacgt cgcaggcggc tatagtggcg caatctattc ttctggtact aatgatactg
      241 gtgccgtcga tttacgtgtc actaacgcca tgtttcgcaa taatatcgct aatgatggca
      301 aaggtggcgc aatttatacc attaataatg acgtctattt aagtgatgat gtttttaaca
      361 ataaccaggc atatacatca acaagttaca gtgatggcga tggcggggca atcgatgtta
      421 ccgataataa tagcgacagc aagcatcctt caggttatac gataataaat aacaccgcct
      481 ttacaaataa cacagccgaa ggttatggcg gggcgatata taccaatagc gtgagggctc
      541 cctatcttat tgatatttct gttgatgaca gctacagcca gaacggcggc gtgttagtcg
      601 atgagaacaa tagcgcagca ggctatggag atggtccttc cactgcggcg ggtggcttta
      661 tgtatctcgg cttaagtgaa gttacctttg atattgccga cggaaaaacg ctggttatcg
      721 gcaatacaga gaatgacgga gctgttgact ctattgctgg taccgggtta atcaccaaaa
      781 caggttccgg cgatctggta cttaatgcag ataacaatga ctttactggt gagatgcaga
      841 ttgaaaacgg tgaagttacc ctgggtcgca gcaactccct gatgaatgtc ggcgatacgc
      901 attgccagga cgatccgcaa gactgctacg gtctgacgat agggagtatt gatcagtatc
      961 agaatcaggc tgagctaaac gttggctcga cccaacaaac ttttgtgcac gcattgacgg
     1021 gctttcagaa tggcacttta aatatcgatg ctggtggcaa cgttactgtt aatcagggca
     1081 gttttgctgg catcatcgaa ggtgctggtc agctcaccat tgcgcaaaac ggcagctacg
     1141 tgctggcagg ggcgcagtcg atggcgctaa ccggcgatat agtcgttgat gatggtgcgg
     1201 tgctttcgct ggaaggcgac gcggcagatc ttaccgctct ccaggacgat ccgcagtcga
     1261 tcgtgttaaa cggcggtgtg ctcgatctct ctgatttctc cacttggcag agcggcacat
     1321 catacaatga tggccttgaa gtcagtggca gcagcggaac ggttatcggc agtcaggatg
     1381 tggtagatct tgcaggtggc gacaatttgc atatcggcgg tgacgggaaa gatggcgtct
     1441 acgtggtggt cgatgcgagc gacgggcagg taagtctggc aaacaataat agttatttgg
     1501 gcacaacaca aatcgcctcc ggtacgctga tggtgagcga caactcgcag cttggagata
     1561 cccactataa ccgccaggtt atctttaccg ataagtaaca agaaagcgtg atggagatta
     1621 ctgccaatgt cgatactcgc tctacgacga ctgagcatgg gcgtgatatt gaaatgcgcg
     1681 ccgacggtga agtggcagtt gatgcggggg tagacacgca gtggggcgca ctgatggctg
     1741 acagcagcgg gcagcatcag gataagggta gcacatttac taaaacgggg gcgggtacgc
     1801 tggagctgac cgccagcggt acaacgcagt cggcggtacg tgtcgaagaa ggcaccctga
     1861 aaggcgatgt tgcggatatc tttccttatg cttcgtcact gtgggttggt gatggggcaa
     1921 cgttcgttac tggcgcggat caggatattc agtcaattga tgctacttcc agcggcacta
     1981 tcgacatcag cgatggtacg gttttgcgtc tgaccgggca ggatacttcc gtcgccctta
     2041 atgcctcact atttaacggc gatgggacgc tggtaaatgc taccgatggc gtgacgttga
     2101 caggcgagct taataccaac cttgaaactg acagcctgac ctatcttgct gacgtgacgg
     2161 ttaatggtga tctgaccaat acgtccggtg cggttagcct gcaaaatggc gtcgctggcg
     2221 atacgctgac ggtaaacggt gattataccg gcggcggtac gctactattc gacagcgaat
     2281 taaacggcga tgactcggta agcgatcaat tggtgatgaa cggtaatact gctggcaaca
     2341 caactgtggt ggttaactcc attacaggga ttggtgagcc gacatcgaca ggcattaaag
     2401 tggttgattt cgcagctgat cccacgcagt ttcaaaacaa tgcacagttc agtctggctg
     2461 gcagtggcta cgtcaacatg ggagcgtatg actacacgct agtggaagat aacaacgact
     2521 ggtatctgcg atcgcaagaa gtaacgccgc catcgccacc tgagccagac ccgactcccg
     2581 atcctgatcc cacgccggat cctgacccaa cccccgaccc ggcccctacg cctgcttacc
     2641 agccggtgct gaatgccaaa gttggcggtt atctcaataa cctgcgggcg gcaaatcagg
     2701 cgtttatgat ggagcgacgc gatcacgctg gtggcgatgg tcagacgctg aatttacgtg
     2761 ttatcggcgg acgttatcat tacacggcgg cagggcaact ggctcagcag gaagacactt
     2821 ctacggtgct gcttagcggc ggcttgttta gcgggtgctg gggcacggat ggcgagtgga
     2881 tgcttggggc ggttggtggc tacagtgata atcagggcga cagccgctcg aatatgaccg
     2941 gaactcgcgc cgataaccag aaccacggtt atgccgttgg gctgacctca agctggtatc
     3001 agcacggtaa tcagaagcag ggggcctggc tggatagctg gctgcaatac gcgtggttta
     3061 acaatgatgt ttccgaacat gaagatggcg tggatcgtta ccattcgtcg gggattatcg
     3121 cctcgctgga agccgggtat cagtggttac cggggcgtgg tgtggtgatt gaaccgcagg
     3181 cgcaggtgat ttatcagggc gtgcagcagg atgattttac cgccgctaac catgcgcgcg
     3241 tgtcacaatc gcagggggat gatattcaga cgcggctggg tttacacagc gaatggcgta
     3301 ccgcggttgg tgtcacacca acattagatc tgaattatta tcacgatccc catgcgacgg
     3361 aaattgaaga agatggcagc actatcagtg acgatgcggt gaagctacgg ggtgaaataa
     3421 aagtgggagt cacgggcaat atcagtcagc gagtgtctct gcgcggcagc gtggcgtggc
     3481 agaaagggag tgatgatttt gcccagacgg cagggttttt gtcgatgacg gtgaaatggt
     3541 aatcgctgtc tgaccatcac aattgccgga tgcggcgtaa acgccttatc tggcctacgg
     3601 agagcatttg taggcctgat aagacgctgc aagcgtcgca tcaggcattg gcgcggccaa
     3661 acatcaacct cacttattct gcgtgtgcag catatagttc acatccacgc cggggccgag
     3721 cttaaaggaa ttagtgatcg ggttgtaatg tagcccggtt atatgtcgct cttttaaact
     3781 ggtctgatcc acccagccaa gcagctctgc cggtttaata aacttcttca catcatgcgt
     3841 acctttgggc accatgcgca aaatatattc cgcgccaacc accgccatca gccatgactt
     3901 gccgttgcgg ttaagcgtcg agaaaaagac atcgccgcct ggtttcacca gttgcgcaca
     3961 ggctctgacc actgactgcg gatcgggtac gtgctccagc atctccatgc aggtcaccac
     4021 atcatactgc ccggcatgtt ttgccgcgtg ctcttccacg gtttcctgca cgtaatccac
     4081 ctgaatgccg ctttccagcg cgtgcagctt tgccacctgc aatggctcaa agcccatatc
     4141 cagaccggtc accgtcgcgc cttcgcgcgc catactctcg gccagaatgc cgccgccaca
     4201 accgacatcg agcacctttt tgccaaataa accgccagca cgctcggcaa tatagcccag
     4261 acgcagcggg ttaatgcggt gcaacggttt gaactcacct tcaagatccc accagcggga
     4321 ggcgacggct tcaaatttag cgatctcttc gtggtctacg ttatggtttt ccggcgattt
     4381 ttcggcattc attggcactt ctactccgta attggcaaga caaacgagta tatcaggcat
     4441 tggatgtgaa taaagcgtat aggtttacct caaactgcgc ggctgtgtta taatttgcga
     4501 cctttgaatc cgggatacag tagagggata gcggttagat gagcgacctt gcgagagaaa
     4561 ttacaccggt caacattgag gaagagctaa agagctccta tctggattat gcgatgtcgg
     4621 tcattgttgg ccgtgcgctg ccagatgtcc gagatggcct gaagccggta caccgtcgcg
     4681 tactttacgc catgaacgta ctaggcaatg actggaacaa agcctataaa aaatctgccc
     4741 gtgtcgttgg tgacgtaatc ggtaaatacc atccccatgg tgactcggcg gtttatgaca
     4801 cgatcgtccg tatggcgcag ccattctcgc tgcgttacat gctggtagac ggtcagggta
     4861 acttcggttc catcgacggc gactctgcgg cggcaatgcg ttatacggaa atccgtctgg
     4921 cgaaaattgc ccatgaactg atggccgatc tcgaaaaaga gacggtcgat ttcgttgata
     4981 actatgacgg cacggaaaaa attcccgacg tcatgccaac caaaattcct aacctgctgg
     5041 tgaacggttc ttccggtatc gccgtaggta tggcaaccaa catcccgccg cacaacctga
     5101 cggaagtcat caacggttgt ctggcgtata tcgatgatga agacatcagc attgaagggc
     5161 tgatggaaca catcccgggg ccggacttcc cgacggcggc aatcattaac ggtcgtcgcg
     5221 gtattgaaga agcttaccgt accggtcgcg gcaaggtata tatccgcgcc cgtgctgaag
     5281 tggaagttga cgccaaaacc ggacgtgaaa ccattatcgt ccacgaaatt ccgtatcagg
     5341 taaacaaagc gcgcctgatc gagaagattg cggaactggt aaaagaaaaa cgcgtggaag
     5401 gcatcagcgc gctgcgtgac gagtctgaca aagacggtat gcgcatcgtg attgaagtga
     5461 aacgcgatgc ggtcggtgaa gttgtgctca acaacctcta ctcccagacc cagttgcagg
     5521 tttctttcgg tatcaacatg gtggcattgc accatggtca gccgaagatc atgaacctga
     5581 aagacatcat cgcggcgttt gttcgtcacc gccgtgaagt ggtgacccgt cgtactattt
     5641 tcgaactgcg taaagctcgc gatcgtgctc atatccttga agcattagcc gtggcgctgg
     5701 cgaacattga cccgatcatc gaactgatcc gtcatgcgcc gacgcctgca gaagcgaaaa
     5761 ctgcgctggt tgctaatccg tggcagctgg gcaacgttgc cgccatgttg gaacgtgctg
     5821 gcgacgatgc tgcgcgtccg gaatggctgg agccagagtt cggcgtgcgt gatggtctgt
     5881 actacctgac cgaacagcaa gctcaggcga ttctggatct gcgtttgcag aaactgaccg
     5941 gccttgagca cgaaaaactg ctcgacgaat acaaagagct gctggatcag atcgcggaac
     6001 tgttgcgtat tcttggtagc gccgatcgtc tgatggaagt gatccgcgaa gagctggagc
     6061 tggttcgtga acagttcggt gacaaacgtc gtactgaaat caccgccaac agcgcagaca
     6121 tcaacctgga agatctgatc acccaggaag atgtggtcgt gacgctctct caccagggct
     6181 acgttaaata tcagccgctt tctgaatacg aagcgcagcg tcgtggcggg aaaggtaaat
     6241 ctgccgcacg tattaaagaa gaagacttta tcgaccgact gctggtggcg aacacgcacg
     6301 accatattct gtgcttctcc agccgtggtc gcgtctattc gatgaaagtt taccagttgc
     6361 cggaagccac tcgtggcgcg cgcggtcgtc cgatcgtcaa cctgctaccg ctggaacagg
     6421 acgaacgtat caccgcgatc ctgccggtga ccgagtttga agaaggcgtg aaagtcttca
     6481 tggcgaccgc taacggtact gtgaagaaaa ccgtcctcac cgaattcaac cgtctgcgta
     6541 ccgccggtaa agtggcgatc aaactggttg acggcgatga gctgatcggc gttgatctga
     6601 ccagtggcga agatgaagta atgctgttct ccgccgaagg taaagtggtg cgctttaaag
     6661 agtcttctgt ccgtgcgatg ggctgcaata ccactggtgt gcgcggtatt cgcttaggtg
     6721 aaggcgataa agtcgtctct ctgatcgtgc ctcgtggcga tggcgcaatc ctcaccgcaa
     6781 cgcaaaacgg ttacggtaaa cgtactgcag tggcggaata cccaaccaag tcgcgtgcga
     6841 cgaaaggggt tatctccatc aaggttaccg aacgtaacgg tttagtggtt ggcgcggtgc
     6901 aggtagatga ctgcgaccag atcatgatga tcaccgatgc cggtacgctg gtacgtactc
     6961 gcgtttcaga aattagcatc gtgggccgta acacccaggg cgtgatcctc atccgtactg
     7021 cggaagatga aaacgtagtg ggtctgcagc gtgttgctga accggttgac gaggaagatc
     7081 tggataccat cgacggcagt gccgcggaag gggatgatga aattgctccg gaagtggacg
     7141 ttgacgacga gccagaagaa gaataatttt acttcttcat gccaaaaggg agctatctcc
     7201 cttgtttgaa ttgaaaagtc caggctgcaa agtctgggct tttgtcgtat tagggcgcgg
     7261 taaagtttgg ctgtgcccgt aaaaaatggc tggctataca caaggaatgt ggcaatgagt
     7321 ggtgaaaaaa aggcgaaagg ctggcggttc tatggtcttg taggttttgg cgcaatagca
     7381 ctgctttccg ctggcgtctg ggcgttgcaa tatgctggca gtgggccaga aaaaacgttg
     7441 tcgccgctgg tggtgcacaa caatctgcaa atcgatctca atgagccgga cctctttcta
     7501 gacagcgact ctctgagcca gcttcccaaa gatctcctca ccattccgtt tctccacgat
     7561 gttctgagcg aggatttcgt tttctattat cagaatcatg ccgatcgtct gggcattgaa
     7621 ggcagcattc gccgcattgt ctatgaacac gatctcacgc tgaaagataa gctcttttcg
     7681 tcactcttag atcagcccgc gcaggcggcg ctatggcacg ataaacaagg ccatctttca
     7741 cattacatgg tgctgatcca gcgcagtggt ttaagcaaac tgctggagcc attgttgttt
     7801 gccgctacca gcgacagcca gttaagcaaa acggaaatca gtagcatcaa gataaatagt
     7861 gaaactattc ctgtttatca gttgcgctat aacggcaata acgccctgat gttcgcgaca
     7921 tatcaggaca agatgctggt gttttccagt acggatatgt tgtttaaaga tgatcagcag
     7981 gataccgaag ccacggcgat cgcaagtgat ttgttgagcg gtaaaaaacg ctggcaagcc
     8041 agctttggcc tggaagagcg tacagccgaa aaaacgccag tacgcctgcg catcgtggcc
     8101 agcgccaggt tgctgggttt tggctaccag cggttaatgc cttcttttgc tggcgtacgc
     8161 ttcgaaatgg gtaacgacgg ctggcacagt tttgtggcgt taaatgatga atccgccagc
     8221 gtagatgcca gtttcgattt tacgccggtc tggaacagta tgcctgccgg agccagcttc
     8281 tgtgtggcgg tgccgtattc acacggtatt gccgaagaga tgctttcgca catcagccag
     8341 gaaaacgaca agctgaatgg ggcgttagac ggtgccgcgg ggctgtgctg gtatgaagac
     8401 tcaaaattgc aaaccccgct gtttgtcggt cagtttgatg gcactgccga acaggcgcaa
     8461 ttgccaggga aactgtttac gcaaaatatt ggtgcgcacg aaagcaaagc gccagaaggt
     8521 gttttgccgg taagccagac tcagcagggc gaagcgcaaa tctggtgtcg cgaagtgagt
     8581 tcccgatacg gtcagtatcc gaaagcgcag gcggcgcaac ccgatcaatt aatgtcggat
     8641 tattttttcc gtgtgtcgct ggcgatgcaa aacaaaacgc tgcttttctc cctcgatgac
     8701 acgctggtta ataacgccct gcaaacactg aataaaaccc gcccggcaat ggtggatgta
     8761 atacccactg atggcatcgt tccgctctat atcaatccac aaggcgtggc gaaactgctg
     8821 cgtaacgaaa cgttgaccag tctgccgaag aatctcgaac cggtttttta taacgccgca
     8881 caaactttat taatgccgaa gctggacgct ttatctcaac aaccgcgtta tgtcatgaag
     8941 ctggcccaga tggaacccgg tgtcgcctgg cagtggctac ccataacctg gcagccgcta
     9001 tgagacacgg gctgctggcg ctgatttgct ggctgtgttg tgttgttgcc catagcgaaa
     9061 tgctgaatgt cgaacaatcc aggctgtttc gcgcctggtt tgtacgcatt gcacaagaac
     9121 agctccgcca ggggccaagt ccacgctggt atcagcagga ttgtgcgggc ctggtgcgat
     9181 ttgcggcgaa cgagacgctg aaagttcacg acagtaaatg gctaaaaagt aacggtttat
     9241 ctagccagta tttgccgcca gagatgacgc taacacctga acagcgtcaa ctggcgcaaa
     9301 actggaatca ggggagcggg aaaaacggcc cctacgtga
//
LOCUS       DAJTYG010000123         9319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTYG010000123 DAJTYG010000000
VERSION     DAJTYG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.123"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            27..878
                     /gene="hchA"
                     /locus_tag="OO568_003423"
     CDS             27..878
                     /gene="hchA"
                     /locus_tag="OO568_003423"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6642639.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(986..2344)
                     /gene="hprS"
                     /locus_tag="OO568_003424"
     CDS             complement(986..2344)
                     /gene="hprS"
                     /locus_tag="OO568_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6642640.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLRELKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLESLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="OO568_003425"
     CDS             complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="OO568_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6642641.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3148..3561
                     /gene="hiuH"
                     /locus_tag="OO568_003426"
     CDS             3148..3561
                     /gene="hiuH"
                     /locus_tag="OO568_003426"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6642642.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3670..4674
                     /gene="msrP"
                     /locus_tag="OO568_003427"
                     /pseudo
     CDS             3670..4674
                     /gene="msrP"
                     /locus_tag="OO568_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4675..5309
                     /gene="msrQ"
                     /locus_tag="OO568_003428"
                     /pseudo
     CDS             4675..5309
                     /gene="msrQ"
                     /locus_tag="OO568_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            5567..6217
                     /gene="zinT"
                     /locus_tag="OO568_003429"
     CDS             5567..6217
                     /gene="zinT"
                     /locus_tag="OO568_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6642643.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7295..7564)
                     /locus_tag="OO568_003430"
     CDS             complement(7295..7564)
                     /locus_tag="OO568_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6642644.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            7621..8289
                     /locus_tag="OO568_003431"
     CDS             7621..8289
                     /locus_tag="OO568_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6642645.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            8605..>9319
                     /locus_tag="OO568_003432"
     CDS             8605..>9319
                     /locus_tag="OO568_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6642646.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPEFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGP"
BASE COUNT         2680 a         2102 c         1905 g         2632 t
ORIGIN      
        1 aactaagcaa caataaggaa tacactatga ctgttcaaac aagtaaaaac ccgcaggtcg
       61 atattgctga agataatgcg ttcttccctt cagaatattc gcttagccaa tatacgagtc
      121 ccgtctctga tcttgatggc gtggactatc caaaaccgta tcgcggtaaa cataaaattc
      181 tggtgatcgc cgcggacgaa cgttatttgc cgaccgataa cggaaaactg ttctcgacag
      241 gtaaccatcc gattgaaacg ttgctgccgt tgtatcatct ccatgctgca ggtttcgaat
      301 tcgaagtggc gaccatttcc ggtctgatga ccaagtttga atactgggct atgccgcaca
      361 aagatgaaaa agtaatgcca ttctttgaac agcataaatc gttattccgt aatccgaaga
      421 aactcgcgga tgttgtcgcc agcctcaacg ctgatagcga atatgcagca atctttgttc
      481 ctggtgggca tggcgcactt attggtttac cagaaagcca ggacgtggct gccgctttac
      541 agtgggcaat caaaaatgac cgttttgtta tctccctttg ccacggtccg gcggcttttc
      601 tggcacttcg ccacagcgat aacccactga atggttattc tatttgcgca ttcccagacg
      661 ccgcagacaa acaaacgcca gatattggct atatgccggg tcatctcacc tggtacttcg
      721 gcgaagagtt gaagaaaatg ggcatgaaca tcattaatga cgacatcacc gggcgagtac
      781 ataaggaccg taaacgtctc accggcgaca gtccttttgc agcgaatgcg ttgggtaaac
      841 tggcggcgca ggaaatgctg gcagcttacg cgagttaatc cctgttacat tgtgatgtaa
      901 gcgcataatc aatcgcagcc acgttaatac tcacgacgtg gctgcgtttt ttcacggtta
      961 acttatggcg tactgaagcc cgatgttaat ttctttgcgg taacgtaatt cggaacacgt
     1021 tatgcttact gagatagtga tacgtagcac ttcctccatg taattcggca atcgctttga
     1081 ctaaagaaag gccaagtccc tgacctacgg aatgacgcga attatctccc cgccaaaatc
     1141 tacggaagag tttttcaggc tcattaattt tcgttccagg actggcgata tcaatattaa
     1201 gagagccgtt ggcatcaaga aaactggtta tatgaatacg cgatttttct ggtgaatatc
     1261 gaatggcgtt aacaataaga ttcgataaca ttcgttgcag taaaatttta tccgcaaaga
     1321 tttgttgatt gcacttgacc ttaaagcaaa tctctttctc gtctgaaaga gattcaagat
     1381 agtccaaaag attttcaact tccttattga gagaaagcga atccagtttc accaaaacat
     1441 tgtttttgtc tgccctggca agaaacagta tattctctgt taaccgcgaa atattttcca
     1501 gctcttcaat gtttccggca attgtttttt gatattcagc gatacttctg atttgactga
     1561 gcgtaacctg attctgaccc agtaatgcat taattggcgt tctaagttca tgagcgagat
     1621 cgtcagcaaa ttgactaagg cgctcaaaat ctttgactaa agcatgatgc attttattca
     1681 acgcctgccc aagaggtttt agttctctcg gtaatgcgtt aacctcaacc ggcactcggc
     1741 tatcgttata attcagcgct tccgttacgc cactcaactt tttgagctct cgcaaccccg
     1801 tcctgattaa caacggactt aatactgagc acagtataat ggcgacaatg caaattataa
     1861 tgctattaat tttatactgt tcaagcatat tatgtctggc tgaagccaat ttagccacag
     1921 taaccgttaa tgatggcgaa acttcatcaa tgttaattcg taaagcatct atctctgtat
     1981 cattaataat gcttctgtaa atgcctgctg cgctgattgt ctcactagca ggtatgttat
     2041 ttaacatgtc atcactgaca tttgtccggt taacaatttt attgatgcca tcactatgaa
     2101 tgatcaaaat atcctgacta acatccatca tccggttaaa gtacacaggt aacgtatctg
     2161 gatttacccc atcaattaac aactgcttga tctgcgctgt ccggttaatg agtgttgtat
     2221 catcgcgcca tttcaactca cttgccaggc cattatagag agtccataca attccagcgc
     2281 cagcaacaga ctgcagaaat ataaaaagca aggttaaacg gacggttata gataatcttt
     2341 tcattatttt tttaccgcta cgaatgaata gcccatcccc cggattgtgg cgattagctt
     2401 ttcaggaaaa ggatcatcaa cttttgcgcg gagcctgcga atggcgacat ccaccgtatt
     2461 ggtatcacta tcaaagttga ttccccaaat ttcactcgca ataaccgttc tgggtataat
     2521 ctcgccagct ctggaggcca gtagccaaag taactgaaac tccttgcgcg tcagtgtaat
     2581 actgatattg tccctgctga cactttgact gacagagtcc atttttaagc cactgatttc
     2641 taatgttgaa ttcagagcgt gatgttgcct taattgtgcc cgaacccttg ctaacaactc
     2701 agaaaatgaa aaaggtttta ccagataatc atttgcccca ctgtccaacc ctctgactct
     2761 gtcatcgaca gaatcccttg cagtaaggca aataacaggg gtttgctttg ccgttcttaa
     2821 cgtttgtaag atctgccagc catccatacc cggaagcata atatccagaa tgatcaatgc
     2881 ataatcatcc ttcagcgcaa gataaagccc atctctgcca tcagaaacgg catcaatgac
     2941 ataacccgct tcggaaagcc cctgcgttac ccattcctgg gtcctttgat tatcttcaat
     3001 aagtagaatc ttcatgaaaa taatatgcca taaatcatat gttatagaaa caacctggtt
     3061 cattacaaaa ttgtaatgct gctgtaaggt taccctggac gttttttcgc tatcctcaaa
     3121 actcattcac atgacaagga tataaacatg ttaaagcgtt atttagtact ctcggtagca
     3181 acggcagcat tttcattacc ttctttggtt tatgccgcac aacaaaacat tcttagcgtg
     3241 cacattttga accagcaaac aggaaaaccc gctgccgacg tgacagtcac tcttgaaaag
     3301 aaagcggata acggctggtt acaacttaat accgccaaaa cagataagga tggacgaatt
     3361 aaggcactgt ggcccgagca aactgcgact actggcgatt accgtgtcgt atttaaaacc
     3421 ggggactatt tcaagaaaca aaatcttgaa agtttcttcc ctgagattcc cgttgaattt
     3481 catattaata aagtgaacga gcattatcat gtgcctttgt tactcagcca atatgggtat
     3541 tcaacctatc gtggcagtta atgcgtaaat atgtaaaagc tgatctctgc tactgttagg
     3601 gaggtgactc cctgacagat taacagtaaa cagctcttgt tggttaacga caaaaaagtg
     3661 tgatggctta tgaaaaagaa tcaattttta aaagaatcag atgttacagc cgagtcggta
     3721 ttctttatga agcgtcggca ggtgttaaaa gcactgggca tcagcgcagc tgcattttct
     3781 ttgcctcacg ctgcgcatgc cgatctgctt agctggttta aagggaacga tcgtccgccc
     3841 gcccccgccg gaaaaccgct ggagttcagc aagcctgccg cctagcaaaa taacctgcca
     3901 ctgacgccag cagataaagt ctccggttat aacaacttct atgaattcgg gctggataaa
     3961 gacgatcccg cggctaatgc tggtagcctg aaaaccgatc catggacact gaaaatcagc
     4021 ggcgaagtgg caaaaccatt gaccctcgat catgatgatt taacccgtcg cttcccgctg
     4081 gaagagcgta tttatcgtat gcgctgcgtg gaagcgtggt cgatggtggt gccgtggatt
     4141 ggttttccgc tacacaaatt gctggcgctt gccgaaccca ccagcaatgc gaagtatgtc
     4201 gctttcgaaa cgatttatgc gccggaacag atgcctggcc agcaggaccg ctttatcggc
     4261 ggcgggctga aatatcctta tgtcgaagga ttgcgtctcg acgaagcaat gcatccgctc
     4321 acactgatga ccgtaggtgt ttatggcaag gcgttaccac cgcaaaatgg cgcgccggtg
     4381 cgactgattg tgccgtggaa atatggcttt aaagggatta aatcgatcgt cagtattaag
     4441 ctgacccgcg agcgtccgcc aaccacctgg aatctggcag cgcctgacga atacggtttt
     4501 tacgccaacg ttaatccgca tgttgatcac ccgcgctggt cacaggctac cgaacgattt
     4561 attggttcag gcggcattct cgatgttcag cgccagccaa cactactgtt taatggttac
     4621 gccgaccagg tggcatcgct gtatcgtggc ctggatttgc gggagaattt ctaaatgcgt
     4681 ctgacagcaa aacaggtgat atggctgaaa gtttgcctgc atcttgccgg attgttgccg
     4741 tttctctggc tggtctgggc gatcaatcac ggtggactgg gtgccgatcc ggtgaaagat
     4801 attcagcatt ttaccggtcg cactgcactg aaatttttgc ttgcggcctt gttaatcaca
     4861 cctctggcac gctacgcaaa acagccgtta ttgatacgca ctcgccgcct gttaggctta
     4921 tggtgctttg cctaggcgac actgcattta accagttacg cattgctgga gttgggcgtg
     4981 aataatctgg cgttattagg taaggagtta attacccggc cttatttaac gttaggaatt
     5041 atcagctggg ttattttgct tgctttagcg ttcacttcaa cccagtcgat gcagcgaaaa
     5101 ctcggcaaac actggcaaca gttgcataac ttcgtctatc ttgtcgcgat cctggcccca
     5161 atacattatc tgtggtcagt gaagattatt tcaccgcagc cactcatcta cgctgggctg
     5221 gctgtactgc tttagcctta cggtataaga agttgctttc ccttttcaac cggttacgca
     5281 agcaggccca caacaaatta tctctataac ttcatattgc cgacaaactt cgcgttatcc
     5341 cggccggatg cggcgcgagc gccctatccg gcccacacag atcgcatcaa gttatgtttc
     5401 gtttgtcatc aacctttcat caccagaata atttatttta tttcaataag atagttcaat
     5461 acacctgaga ataccatgct ctcatttcct aatgagttgt tgcatttttt catatgttac
     5521 aatataacat tacacataat gtacattaac tctggaggaa attgttttgg cgattcgtct
     5581 tcataaactg gctgttgctt taggtgtctt tattgttagc gctcctgcct tttcgcatgg
     5641 tcatcattct catggcaaac ccttaacaga ggtcgaacaa aaagctgcta atggtgtttt
     5701 tgatgatacc aatgtacaaa accgaacgct cagtgactgg gatggagtct ggcaatccgt
     5761 ttacccttta ctgcaaagtg gcaaacttga tcccgtcttt cagaagaaag cggatgcaga
     5821 taaaaccaaa acatttgctg aaattaaaga ttattatcgc aaaggttatc cgacagatat
     5881 cgaaatgatt ggcattgagg acggcattgt cgaattccat agaaataatg aaacaacatc
     5941 ctgtaaatat gattacgatg gatacaaaat actcacctat aaatcaggca agaaaggcgt
     6001 tcgttactta ttcgaatgta aagatcctga aagcaaagcc cctaaatata tacaatttag
     6061 cgatcatata attgcaccac gaaaatcatc tcattttcac atttttatgg gtaatgattc
     6121 ccagcaatct ttattgaatg aaatggaaaa ctggccaacg tattatccct atcagttgag
     6181 tagcgaagaa gtggtcgagg agatgatgtc tcattgattt ttttgcgctg acttcagtta
     6241 tttaacagaa cagtgcctgg tagtaacaac aataatttac caagacaaat aagtaagaca
     6301 aactcagaca cgctcatact tgagtcgtgt cttttttatt atgtctgtag aaaaaaactg
     6361 agaaaaaggc gaacaataaa tacacaactc agccatacag actggcagcg caaatgtttc
     6421 caggagcaag tcatctgtca tggtaaagct aaccagaaat ataggttaat tttggtgttt
     6481 aaaccttttt tacttgttag tatgatacaa aggctttcaa aaaagctggg aaattcaaca
     6541 actcttggaa gttatggact ttgtcccgcg gctttaatct gacggccgcg ttcctttttt
     6601 ggctcaatta ttgacccctt ctctacgcaa cttcagttcc cgccagcaac tttgcggcag
     6661 tttcatagga tcaatgtcta aaagaataat ggtgaccgat aagaaaacga ctgaataact
     6721 gtagattttc gctcgaaacc ttcctgtcag ctccatagcg aatcaagcgc tgaatgtcac
     6781 agtatcgaac agaaaacagt gacgatctaa cccttcaaga atattctacg attgttctgt
     6841 ttaggaaaag caaggcggga agtcgggaga taagtcattg ataaagtggc ggagagaggg
     6901 ggatttgaac ccccggtaga gttgccccta ctccggtttt cgagaccgat gatacagagt
     6961 cgaaaaatca aagagttaaa aatttttctt ggaaaaaatg tcttaaaatc gtacacgaaa
     7021 aatcaatgaa ttacataaca ttaaagaaga atattccaag ccttcttgag tccaccacct
     7081 gttttcaggt gtcattcaga cgtgatttgc cccagattgg gaaagcacgc ttttttctca
     7141 gcttattccg ttttgcctgt gtgttgaata attccactgc agttatataa aatcggtaac
     7201 ggctggaaat cattcaatac tcgcactatc gaaagttcac cagccaaccg cagcacgtcc
     7261 tgcatacgtc gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggcc
     7321 actcaatatc aggtgcagtt gatgtatcaa cacggttcag caacacccga tacttcttcc
     7381 aggcttccag caacgagttt tcttcctccg ttgcgatctc cagatctaca gcatcctgaa
     7441 gtggcgcaat atgctcactg aatccctgga tgtagaactg tgtggtgacg gtcttccagc
     7501 cattcggctc ctgctgtatc gaagcatacc aggctatttc aatatcgcta tgctgcggca
     7561 gcatttaacc ccttgtaatt catcgccata attgatttaa ttcacaaata aaactataat
     7621 atggtgaaat taatgaaaaa aaacacagat gatggggcta aaatttacac accacttacc
     7681 ctaaagcttt atgactggtg ggttttggga gtatcaaatc ggcttgcatg gggatgtcct
     7741 acaaaggaac accttcttcc acactttctg gaacatttag gtaacaacca tctggatatt
     7801 ggtgttggaa ctgggtttta ccttactcac gtacctgaga gtagtctgat atctttaatg
     7861 gatttgaacg aagctagcct gaacgcggca tctacaaggg ctggggaatc aaaaattaaa
     7921 cataaaatta gccatgatgt ttttgaacct tatcctgcgg cgttacatgg tcaatttgat
     7981 tccatttcca tgttttacct tcttcactgc ctgcctggaa atatatctac aaaaagctgt
     8041 gtaatacgca atgcggcgca ggccttaact gatgatggaa ctctatacgg agccacaatt
     8101 cttggcgatg gagttgtgca caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag
     8161 aaaggcatct tttcaaacac aaaagattcc gaagaaggct taacacatat actctcagag
     8221 catttcgaga atgttaaaac caaggttcaa ggtactgtag taatgttttc cgcttcaggg
     8281 aaaaaatagc atccaaccgc agcacgttct tgcttaagac gtgctacggt ttcctttacg
     8341 atttggggtt gcgactttac ctctattgat aatgcattcc ggcagaacgt tcaaatatga
     8401 acgtacgata tttaactaac cgaaaaacaa aatatagcac aggcgagaca tttaccatct
     8461 aagaaaatag tatcgttttt ttacagttaa atcaacatca cttccttaaa atgaaaacaa
     8521 taaatttcaa tcctgaaaca ttttatttca gcttattatt ccccagtatc acattttatc
     8581 aggatatcct tctgcgaggt tattatgctt cctgtaaata atccccccct atccactgga
     8641 aacgtctctt tttacagaac tacatcaatc gacaatgttc acaataatta tctctccgaa
     8701 tgggttgaat ggactaaaaa cagcatttcc ggagaaaaca gggaaactgc ttttacccgg
     8761 ctccaattat gtctggagaa cagtgaaaca tcgttggact tatcttgttt aggtctcaga
     8821 tctctaccac gattgcctga caatcttgat gaaattaatg taagcaataa ccaactatca
     8881 atgctccccg agctaccaag ggcattgaaa gagctgaatg caagcagtaa tcaattatct
     8941 gcacttcctg aattaccagt gtcgctggaa tatataaatg tgagtgataa ccatttgttc
     9001 gcacttcctg aattacctgc gtcactagaa tatattaatg taagtgacaa tcacctgtct
     9061 gtacttccga ggttaccaat gtcattggaa ttacttgatg cagccagaaa tgctttggaa
     9121 gtaataccag agtttccaga aagagatgat catattataa gaatattctg gcttaatcag
     9181 aaccggatca cggcaattcc ggaaagcata cttggcctca gttctgatag cgttgtcaat
     9241 cttagagaaa atcaactatc tcccagaata atgcaaactt tgttacaaca aaccgcccaa
     9301 ccggactacc acggcccac
//
LOCUS       DAJTYG010000124         9161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTYG010000124 DAJTYG010000000
VERSION     DAJTYG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.124"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..924
                     /locus_tag="OO568_003433"
                     /pseudo
     CDS             1..924
                     /locus_tag="OO568_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1176..2093
                     /gene="oxyR"
                     /locus_tag="OO568_003434"
     CDS             1176..2093
                     /gene="oxyR"
                     /locus_tag="OO568_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6642647.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="OO568_003435"
     CDS             complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="OO568_003435"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6642648.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCKILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3797..4447
                     /gene="fabR"
                     /locus_tag="OO568_003436"
     CDS             3797..4447
                     /gene="fabR"
                     /locus_tag="OO568_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6642649.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4447..4806
                     /locus_tag="OO568_003437"
     CDS             4447..4806
                     /locus_tag="OO568_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6642650.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="OO568_003438"
     CDS             complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="OO568_003438"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6642651.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6060..6250
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6315..8159
                     /gene="btuB"
                     /locus_tag="OO568_003439"
     CDS             6315..8159
                     /gene="btuB"
                     /locus_tag="OO568_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6642652.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNCTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8104..8961
                     /gene="murI"
                     /locus_tag="OO568_003440"
     CDS             8104..8961
                     /gene="murI"
                     /locus_tag="OO568_003440"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6642653.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2096 a         2219 c         2469 g         2377 t
ORIGIN      
        1 ctgggtattg gcgaagcgcc atttatgcct gcgggagtaa agtcgatcac cgactgatat
       61 gcacaaaaag aacgcggcac ggcgctgggg atttttaact catctaccgt tatcggtcag
      121 gctatcgcgc ctcctgctct tgtattgatg cagctggcat ggggctggcg gacgatgttc
      181 gttatcatcg gcgtggcagg gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac
      241 cgggcgcagt ttgtcctgac tgacgaagaa cggacgtatc tctccgcgcc ggttaaaccg
      301 cgtccacagc tgcaatttag cgagtggctg gcgctgttta agcatcggac aacctggggg
      361 atgattttag gtttctctgg cgtcaattat accggttggc tctacattgc gtggttaccc
      421 ggttatttgc aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt
      481 cctttcctcg cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg
      541 aaaaaaggct acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg
      601 tcggcattag gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt
      661 atctcaatgg cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag
      721 gtgatggtgt cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt
      781 gtttttgctt cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt
      841 aatctggcgc tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt
      901 attgtcaaag atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag
      961 aataaaaaaa agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag
     1021 ttgagaaact ctcgaaacgg gcagtgactt caagggttaa aagaggtgcc gctccgtttc
     1081 tgtgagcaat tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta
     1141 tcgccatgaa ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc
     1201 tggtggcatt ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc
     1261 agccgacgct tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg
     1321 agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc
     1381 gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggtgagacga
     1441 tgtccggacc gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata
     1501 ttatcccgat gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga
     1561 cccaccagtt actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg
     1621 tgaaagagag cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta
     1681 tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtact gatggccgat ctggcagggg
     1741 aaaaactgct gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct
     1801 ttgaagccgg ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca
     1861 acatggtggc ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc
     1921 gcaaacgcga tggggttgtt tatctgccat gcattaagcc ggaaccacgc cgcactattg
     1981 gcctggttta tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca
     2041 tccgcgcaag aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt
     2101 aacgcagcta cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag
     2161 tactcaatag tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt
     2221 tcggcagcgc gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac
     2281 aaaattttca gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac
     2341 tgggcgcggc ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc
     2401 acagagctga tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg
     2461 gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat
     2521 gccaggctcg gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga
     2581 tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac
     2641 gccagcgaat cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt
     2701 tttttacccg acttcagatg catgatcaca ccgtcgtcac agctttcgat cttctcgtac
     2761 tcttcgttgt gacgaatcac tacgccactg ttccagaagt gataggagag agaatctgac
     2821 atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca
     2881 tccataccgc ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt
     2941 acatggcgcg gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg
     3001 gtgaaatcaa catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca
     3061 gcggttagtg tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca
     3121 aagcgagcgt tcccctgcaa tattttacag tgattacgtt cgtaaaatcc ctgacgcatg
     3181 cgcgtttgtt gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg
     3241 agcagtcggg aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg
     3301 gcgtgacgga gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt
     3361 tgataacgct cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct
     3421 tcgccgccgg ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta
     3481 gggcttacct gttcttatac ataaaatcaa cataatggta acattctatc gcgggtaagc
     3541 caattgatcc ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct
     3601 tacttttgcc ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag
     3661 gtttccgctg tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt
     3721 cacaatactg gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg
     3781 caaggattca gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc
     3841 gctggtggaa gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt
     3901 gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga
     3961 cgtagacgaa ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat
     4021 gcgccaggca cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac
     4081 atttatggag ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc
     4141 cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga
     4201 acttgcggac tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc
     4261 cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga
     4321 acaacgtcgg caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc
     4381 ttattactgg tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga
     4441 cgagtaatga aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggc
     4501 ttatcgatta atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc
     4561 ccgattattt ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc
     4621 atgtcggtag gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac
     4681 agcacggtag ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc
     4741 tccgtcatta tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct
     4801 gagtaatcgg tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag
     4861 taatacgccg cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc
     4921 gaccttgtgc gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga
     4981 gatatacaga atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct
     5041 gcgcggtggg tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag
     5101 gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat
     5161 aatttgtacg ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg
     5221 tttggcgatt tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt
     5281 accgttgccg cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc
     5341 ccattccagc atttgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg
     5401 acgatagatc atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag
     5461 cgcgattttg gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag
     5521 tgcatcgcgc agtgcttccg cttcctgacg ccactcatca tccagctttt tatggtacag
     5581 cagggaaacc accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg
     5641 gcgcagaacg ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgatgag
     5701 ttcactggcg gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat
     5761 gatgtgatac aggtcatcgc catcgtgcca gatgcggaac tccgcgcgca tccggtaatg
     5821 actgaccggc gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg
     5881 caaacgtacc actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg
     5941 ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat
     6001 gtccagatct tgatgaattc ctatttgtga gctacgtctg gacagtaact tgttacaacc
     6061 tgtagcatcc acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctagagctg
     6121 acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg
     6181 aagtcatcat ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggccaacg
     6241 tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt
     6301 gtggatgctt tacaatgatt aaaaaagctt cgttgctgac ggcgtgttcc gtcacggcat
     6361 tttccgcttg ggcacaggat accagcccgg atactctcgt cgttactgct atccgttttg
     6421 aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt gacccgtcag gatatcgacc
     6481 gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct tccgggcgtc gatatcaccc
     6541 aaaacggcgg ttcaggtcag ctctcatcta tttttattcg cggtacaaat gccagtcatg
     6601 tgctggtgtt aattgatggc gtccgcctga atctggcggg ggtgagtggt tctgccgacc
     6661 ttagccagtt ccctattgcg cttgtccagc gtattgaata tatccgtgga ccgcgctccg
     6721 ctgtttatgg ttccgatgca ataggcgggg tggtgaatat catcacgacg cgcgatgaac
     6781 ccggaacgga aatttcagca gggtggggaa gcaatagtta tcagaactat gatgtctcta
     6841 cgcagcaaca actgggggat aaaacacggg taacgctgtt gggcgattat gcccatactc
     6901 atggttatga tgttgttgcc tatggtaata ccggaacgca agcgcagaca gataacgatg
     6961 gttttttaag taaaacgctt tatggggcgc tggagcataa ctgtactgat gcctggagcg
     7021 gctttgttcg cggctatggc tatgataacc gtaccaatta tgacgcgtat tattctcccg
     7081 gttcaccgtt gctcgatacc cgtaaactct atagccaaag ttgggacgcc gggctgcgct
     7141 ataacggcga actgattaaa tcacaactca ttaccagcta tagccatagc aaagattaca
     7201 actacgatcc ccattatggt cgttatgatt cgtcggcgac gctcgatgag atgaagcaat
     7261 acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg tagtattggt gcgggtgtcg
     7321 actggcagaa acagactacg acgccgggta caggttatgt tgaggatgga tatgatcaac
     7381 gtaataccgg catctatctg accgggctgc aacaagtcgg cgattttacc tttgaaggcg
     7441 cagcacgcag cgacgataac tcacagtttg gtcgtcatgg aacctggcaa accagcgccg
     7501 gttgggaatt catcgaaggt tatcgcttca ttgcttccta cgggacatct tataaggcac
     7561 caaatctggg gcaactgtat ggcttctacg gaaatccgaa tctggacccg gagaaaagca
     7621 aacagtggga aggcgcgttt gaaggcttaa ccgctggggt gaactggcgt atttccggat
     7681 atcgtaacga tgtcagtgac ttgatcgatt atgatgatca caccctgaaa tattacaacg
     7741 aagggaaagc gcggattaag ggcgtcgagg cgacagccaa ttttgatacc ggaccactga
     7801 cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc gattaccgac acgccgttgt
     7861 tacgccgtgc taaacagcag gtgaaatacc agctcgactg gcagttgtat gacttcgact
     7921 ggggtattac ttatcagtat ttaggcactc gctatgataa ggattactca tcttatcctt
     7981 atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct tgcggttgcg tatccggtca
     8041 cctctcacct gacagttcgt ggtaaaatag ccaacctgtt cgacaaagat tatgagacag
     8101 tctatggcta ccaaactgca ggacgggaat acaccttgtc tggcagctac accttctgaa
     8161 ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg ggttgtcggt ctatgacgag
     8221 atccggcatc tcttaccgga tctccattac atttatgctt tcgataacgt cgctttcccg
     8281 tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg tggcaattgt caccgcggtg
     8341 caagaacgtt atccccttgc gctggctgtg gtcgcttgca acaccgccag taccgtttca
     8401 cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg gtgtcgtgcc ggcgattaaa
     8461 cctgctgcac gtctgacggc aaatggcatt gtcggattac tggcaacccg cggaacagtt
     8521 aaacgttctt atactcatga gctgatcgcg cgtttcgcta atgaatgcca gatagaaatg
     8581 ctgggctcgg cagagatggt tgagttggct gaagcgaagc tgcatggcga agatgtttct
     8641 ctggatgcac taaaacgtat cctacgcccg tggttaagaa tgaaagagcc gccagatacc
     8701 gttgtattgg gttgcaccca tttccctcta ctacaagaag aactgttaca agtgctgcca
     8761 gagggaaccc ggctggtgga ttctggcgca gcgattgctc gccgaacggc ctggttgtta
     8821 gaacatgaag ccccggatgc aaaatctgcc gatgcgaata ttgccttttg tatggcaatg
     8881 acgccagaag ctgaacaatt attgcccgtt ttacagcgtt acggcttcga aacgctcgaa
     8941 aaactggcag ttttaggctg atttggttga atgttgcgcg gtcggaaaat tattttaaat
     9001 ttcctcttgt caggccggaa taactcccta taatgcgcca ccactgacac ggaacaacgg
     9061 caaacacgcc gacgggtcag cggggttctc ctgagaattc cggcagagaa agcaaaaata
     9121 aatgcttgac tctgtagcgg gaaagcgtat tatgcacacc t
//
LOCUS       DAJTYG010000125         9029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTYG010000125 DAJTYG010000000
VERSION     DAJTYG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.125"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(1..100)
                     /locus_tag="OO568_003441"
                     /pseudo
     CDS             complement(1..100)
                     /locus_tag="OO568_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            98..422
                     /locus_tag="OO568_003442"
                     /pseudo
     CDS             98..422
                     /locus_tag="OO568_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(452..3364)
                     /locus_tag="OO568_003443"
     CDS             complement(452..3364)
                     /locus_tag="OO568_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642654.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGSIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3367..3483
                     /locus_tag="OO568_003444"
     CDS             3367..3483
                     /locus_tag="OO568_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6642655.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3847..4689
                     /locus_tag="OO568_003445"
     CDS             3847..4689
                     /locus_tag="OO568_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6642656.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSLGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGRECLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4692..5780
                     /gene="capU"
                     /locus_tag="OO568_003446"
     CDS             4692..5780
                     /gene="capU"
                     /locus_tag="OO568_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6642657.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5785..6735
                     /gene="virK"
                     /locus_tag="OO568_003447"
     CDS             5785..6735
                     /gene="virK"
                     /locus_tag="OO568_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6642658.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNQIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6800..7744
                     /gene="lpxM"
                     /locus_tag="OO568_003448"
                     /gene_synonym="msbB"
     CDS             6800..7744
                     /gene="lpxM"
                     /locus_tag="OO568_003448"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6642659.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(7898..8929)
                     /locus_tag="OO568_003449"
     CDS             complement(7898..8929)
                     /locus_tag="OO568_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR6642660.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGGNSKSSALFISLPKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRGIYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT         2354 a         2077 c         2163 g         2435 t
ORIGIN      
        1 gaagggcatc aagctcctgc tccgacatac ctgcaggcaa aggccatgaa agccctgatt
       61 tccggaagcg agcgaacagt ccacaaactg ttgaacgtgg tgtcagcggc ggacagggat
      121 aagccctcgt cagcagttcc gtcagcactg cgacagcgtt ctttacccgg tcaaaacagc
      181 gttgcggtgc accacgcctg acggatgaac tgcgtgctca gggttaccac tttaacgtaa
      241 aaaccgtggc ggcaagcctg cgccgtcagg ggctgagggc aaaggcctcc cggaagttca
      301 gcccggtcag ttaccgcgca catggcctgc ccgtgtcaga aaatctgctg gagcaggatt
      361 tttacgccag tggtccgaac cagaagtggg cgggagacat cacgtactta cgtacagatg
      421 aagttcggtt acatcctgtc agcacggaac ctcacgcttt ttgagcggca cgacggatcc
      481 cccccaccca gtatccgaca gacttttcac tgcgttcgta aaaacccttc ttccctccca
      541 gcatatccac taattccggt gacatatcct gcagataagt ctgcaaatca tgccccagat
      601 cttccggtga ctgcaactca taaaccgcgc gccacacatc accctgcctt attttaagcc
      661 ccgggttaag aatatactgt ccgtggttca gcctcagaaa aagctttttt ccataaggat
      721 ttttgctgtt aacttcatgt tgagagagct ggctgctgat ataactcctt cgtttgcggc
      781 gttccggcag caatgcttca ggaagatgaa ccacacactc tgccagtcgt gccgcactga
      841 aggcttccga aaaaaacaga gcacttccca gatgcgtgtg gaacagcgcc tgcatcagga
      901 tcaccagaaa cattgccatc tgccggttgt ccagtttgat gaggcgtcct tccacctgca
      961 gtgacagact ttcaggtttc agcagggtat acaactgcgc acttttctgt tgcgcataac
     1021 ggggcgtact gaccgccgct gacagaactt gatgataagc cgccagcccg tcattaccgg
     1081 tcagcaaggg atctgcacca cgctccagca gtaactgaac cagtgcaata ttacctgccc
     1141 gggctgctga catcagcgga gtaagattaa acggcgaacg gtgatcaaca ccataacgtt
     1201 caatatcccg caacacggca accgggttat tcattgagta tggccggaaa tacttcagct
     1261 ccagtaactg cagagtcttc gttctgggct gccttgtggg cttaaaatct tcccggtaca
     1321 gtgcattata cagcgggaac agggaataaa tcagactgta ctccagcgcc tgcagttgta
     1381 tggttttatc ggctgtacct gcgaggatat gctgtttcca ctgtcgtgca tcctctgcgg
     1441 tgataatctg ccatggcatc tcagatgtct gcaggatccg gctacggatg gtgtccgcct
     1501 gctcctgctt cccctgtttt tcaagcagat gtgcggtttt ctgccactca tcacggctgg
     1561 aaatctcttc ctgcagattc agaggctctt cctgatgcgt gagcgcaagc agactccaca
     1621 gaggatgctc cacctgctgt tcaacaagat acacattgtg tgtagcacgg gtgagcgcaa
     1681 catacagcgc gttggtaaaa aatttataga tttccgctga acgatcctgc ctgtcccgcg
     1741 gacgggaata acgggcctcg ccctcaagat cagccggtgt aagaccctcg caaatatcat
     1801 caaatgcctg ccgggcagcg cttactatat tgtaaaggat caccgtttcg tattccagcc
     1861 ccttaacctc ctgaaccgaa aagaccagcg gcgtactgaa ccagcatcgc gcctgtgctt
     1921 tctgttccgg atgcatcaca atgaccgcta cacgattcga aagtgaggtt ttggcattca
     1981 gttcctgctt tgtctcttcc ctgtcgtcca gcaaccggat gctcccttct gcctgaccgc
     2041 agcttctgac aaaatggtga ctttcccgat caatagcact aaagcggacc tgtttgagtc
     2101 tcagcaaccg gttagccagt gcagtgacgt ggtgcccgtt acggtaattc gcctgcagga
     2161 tattaacggt tgtgtcgttt ccctgttgct gccggaaaaa gagacttttg agcgaactcc
     2221 atgagaaaaa gctcggatga actatctgat tggcatcacc acagagtaaa aactgaccgg
     2281 gatgacgtag cgttttcagt accagttgca gttgaacagg cgtcatatcc tgaacttcat
     2341 caacaaaaat gacatcataa cggggagttg ccaacgaaag atactgatga ctgagcagat
     2401 tactgtcata ctgatgcgac tgtttcagcc atgcgatata acgttcaaac aggacataga
     2461 ccgtcgggcg gtcctccatg ccatagagcg actgccgtat ccccagtgac agatacgcct
     2521 cccgggacag cggcccattc ccggaggcaa ccgcaccgat cacgcccctg aactcctcat
     2581 acagggtatg ggcagccccc agtgcggcga tagccctgtt gcggggcagc cagtcgctga
     2641 acgcactgaa agtcacctcc ctgccttccg gaatgcgcag agtttccaga aattccgtca
     2701 gcgaaagaaa gtcaatattc tgtacctcac tcccttcccc tgaagcgtca tagagagtac
     2761 gcgctttttc caccagaaat gaggaaaggc tgagatacag tatatcacca gcagcctgct
     2821 tcattttttc cagtaccagc gccgtcttac cacttcccgc cgagccaaca atcaccagcg
     2881 gtagcgggtg ttcgtacagt gcctgctgat catcatcaaa ggacagcatt ttatccaggc
     2941 ggtggaaacg cccatgagac ggattgatat acgtcagcgc ttcagtcgcg atatcaacag
     3001 gatccggcac tggttgttgt tgtaagcgcc cttcatcaat caccacgttt ctgcgaagaa
     3061 aacgtgaagt gttataggca tgattgcgaa tatattccag aacaaggcag atagtttcac
     3121 cgtggtagcg atacagcgaa aacagtaatc ggtcccggat actgagccgg gcgcggtaaa
     3181 gattggtgtc aattttacga acatcagcct gaacaaagtt atcggtggca atagcctgtg
     3241 caaatttttt ataacccggg atcgtgtcag gattcagagt atgataaaga cagattttca
     3301 tggctgccgg tttccgtcgt gcaaaaaaca tgctgatttt acgaaggaat gccttactga
     3361 acaaccttgc ccggaatacc ccccgaaatc agatggaaca atcagaaaaa tgctctgcag
     3421 ttatggaata aagaaggtgg ggtggtacgc cgggagggcg atccgctgga aatggcgcag
     3481 tagctgcagg cctgtcatga tgccggaata gaagttcgtg tacaggttaa tgaaagcgtc
     3541 accccgtaaa taaactctgc gctgaaattc ctttttttca ttggagcacc tgtgttgttc
     3601 tgaggtgaac atatcacctc tgttcaggta gccaaattca gtgtgccacc tcaaggttct
     3661 tagtgagtcg tttttaaaag atatgataat atatcattgt ttatgaattg ttgatgttta
     3721 atttagtaaa ttatatgaaa tatgccatgg gtttgctatg ttattcatcc agtatctgga
     3781 aataaaatgc accagcttta ttgggttttc cttttgtctc tgcttactag gtgccagaaa
     3841 ctttaaatgt taaacgaagg gggcattttg ttcaaagcca atcatgttcc ggtattaatg
     3901 tatcatcatg tcagtcattg ccccggactg gttacacttt ctcccgtgac attccggaag
     3961 caaataaaat ggttggctga aaacaactgg aaaacactgt catcagatga actggagttt
     4021 ttttatcggg gcggaaaact cccccggaaa agcgttatgt tgacatttga tgatggatac
     4081 cttgacaact ggtttcaggt atatccttta ttaaaagagt ttaatctcaa agcacatatt
     4141 tttttaatta ccggttttat tgggaatggt ccggttcgtc actctcttgg aaaggaatat
     4201 tctcatcgcg attgcgaaca tcaaatagcg acagggaatg ctgataatgt tatgcttcgc
     4261 tggtctgaag taaacgaaat gttgcaatct ggtctggtcg aatttcatgt tcatacgcat
     4321 actcatacac gctgggataa aaagttcagt tcacgagaag aacagtgtaa gcatcttcgg
     4381 caggacttgt tgagcgggag agaatgtttg aaagaaatga caggaaaatg cagtaaacat
     4441 ttgtgttggc cggaaggtta ttataacaag gattatattc aggtcgctga agaacttgga
     4501 ttctattatt tatatacaac agaaagaaga atgaatgctc ctgctaaagg aactacacgg
     4561 ataggaagaa taagcaccaa agaacgggaa agttgcgcat ggctgaaaag acgacttttt
     4621 tattatacaa caccgttttt ttcatcgttg ctggcctttc ataaggggcc aagattaccg
     4681 gatgattaat aatgaatata ctatttacgg aatcatcacc gaatataggt ggtcaggaat
     4741 tacaggctgt tgctcaaatg aaggccctga agaaaatggg gcattcagtt ctgcttgtct
     4801 gcagggaaaa cagcaaaatt gcttttgaag ccagtaaatt gggaattgat atcacattcg
     4861 cgttatttcg aaacagtctt cacatcccta ctgcatggag attactcgga atagttcatg
     4921 gttttcagcc caatgcaatc gtttgtcaca gtgggcatga tagcaatatt gttggtttag
     4981 tacggttatt cactaggaaa catccattca gaattatcag gcaaaagaca tatttgacac
     5041 gaaaaacaaa agttttttca ataaatcatt tttgcgatga ggtgattgtt cccggaacaa
     5101 gtatgaagac acatctggag caggaaggat gtcgaacccg ggttactgtt gtgcctccag
     5161 gctttgattt ccagaaatta tacgttgatt cccgaaacag tttgcctcca aatgttcttt
     5221 cttggctggc gtcccgaagg ggatgccccg ttattgctca ggtaggaatg ttgcgcccgg
     5281 aaaaagggca cgaatttatg ttgaatttac tgttccattt aaaaatgaat ggacgacagt
     5341 tctgttggtt gattgtgggg tctggttcgc ctgaactgcg ggagcattta cagtatcaga
     5401 ttgacagtat gggcatgcat gatgatgttt ttattgctga caatgttttt cctgccgccc
     5461 ccgtatatcg ggttgccagt ctggtggttc tgccttcaga aaacgaatcg tttggtatgg
     5521 tactggcaga agcatcggca ttttctgtgc ctgtactggc cagtcagatt ggtggaatcc
     5581 ctgatgttat tcagaacaac cagaccggga cattgttacc agcaggtaat aagcacgcat
     5641 ggatgtgcgc cctgaatgat ttttttaatg accctgggcg tttttatcag atggctcgcc
     5701 aggcaaaaca ggatatagaa gagcggtttg atattaataa aactgcgtta aaaatactca
     5761 cattagcgaa gcacaagtaa caatatgttt tctgtaagta acttatcatt tatcggtttc
     5821 cttaaaagga ttgttttttc ctcagattca ctcccgggga agtgggaaca cagaaaattc
     5881 cggttcatgt acattttgcg atgtgctata aatccggttg ccagtattcg atattattac
     5941 gaactgcgtt ccttgcagtg cattgaggat attctggcaa tacaacccac gttgccagca
     6001 agaattcatc gaccttattt gcataaaggg gggcgagcct ggtcccgggg gcaatatatt
     6061 cttgagcatt atcgctttgt gcaaaatttg ccagaaaaat attctgaatt tttgtttcca
     6121 caaaaatcgg tatcattagt ccaattcatc gggaaagatg gagaggattt tgatatccag
     6181 tgttctccca gtggttttga ccgggaagga gagttgatgt tgtcattgtt tttcaatcaa
     6241 atagtgattg cccggttaac attttcagtc attctgactc aaaatggaca caccgctttt
     6301 attggcggat tacagggagc ccccaaaaat accggacctg atgttattcg gtgcgcaacc
     6361 cgggcctgtt atgggctttt cccaaaacgg attatttttg aagcattttg tgcgttgatg
     6421 aaagcttgta atgtctctga gtgtctggca gtcagtgagc acagtcatgt tttccggcaa
     6481 ttgagatact ggtatcagaa acgcaaaacc tttgttgctg tctacagtga tttctgggag
     6541 tctgtagcag ggaaaacctg tggtgactgg tacaaattac caacacaggt ggttcgaaag
     6601 ccgctaagca atatagctag taaaaaacgc tctgagtatc gaaaacgata cgctttgctg
     6661 gattatatcc atgaaactgc tatccgctct ttggatgcat atcctgtaaa ctcagaacat
     6721 caggacttaa attaaggttt gatgtattct ctgaataaaa tattaatgat gattttggta
     6781 ggggcattcg cactaaataa tgaaaaaata caaatccgag tttattcctg aatttaagaa
     6841 aaattatctt tcccctgttt actggtttac atggttcgtt ttgggaatga ttgcaggtat
     6901 ttcaatgttt cccccttcat tcagagatcc tgtcttggcc aaaatagggc gttgggtggg
     6961 cagattgagc agaaaagctc gtcgcagggc gacgattaat ttatcgcttt gtttcccgga
     7021 aaagagtgat acagaacggg aaataattgt cgacaatatg tttgccacag cattgcaatc
     7081 catagtgatg atggcagaac tggcaattcg tggtccggaa aagttccaga aacgagtgtt
     7141 ctggaaaggc ctcgaaattc ttgaggaaat ccggcacaat aacagaaatg tgatttttct
     7201 ggttccccat ggctggagcg tggatattcc tgcaatgttg ctggcagctc agggggaaaa
     7261 aatggccgcc atgtttcatc agcaacgaaa tccagtgatt gattatgtct ggaattctgt
     7321 acggcgtaaa ttcggggggc gcttacattc ccgggaggat gggataaaac catttattca
     7381 gtcagtacgc cagggatact gggggtatta ccttccagat caggatcatg gtcctgaata
     7441 cagtgaattt gctgattttt ttgcgaccta taaagcgaca ttaccaatta ttggacgtct
     7501 gatgaacatc agtcaggcta tgattatacc gcttttcccg gtttatgatg aaaaaaaaca
     7561 tttcctgact attgaagttc ggccaccaat ggatgcatgc attgccagcg cggacaataa
     7621 aatgattgcc cgacaaatga acaaaacagt ggaaattttg gtggggtcac atccggaaca
     7681 gtatatctgg gttttaaaat tgttaaaaac gcgcaaatca aacgaagcgg acccgtaccc
     7741 ttgaaattat aaagtacagg tatttccact agttgtttct tacaggttac caatcgaaac
     7801 acatccccct tccgtgtgtt gcccgtttgg cacggaggac tgtgtagatg cagttcgtta
     7861 aatttaattt atgcctaaat agctgcgctt aataccgcta cacttttgcc agcccatgtt
     7921 tgcctccggg gaatgggctg acggtttcca taaaatggcg aacttttttc aacagttgcc
     7981 acattgagcg gcactgatga ttacgcgtta ttgtgtcgtg aagtgcctgc catagccgtt
     8041 caacatgatt cacccatggc gagtaaaccg gctgataaat gcccctgaac ttcgggttct
     8101 ccttcagcca gctctgtgtt tcccggcttt tgtggataat gtagttgtcc acgatcagcg
     8161 tgatggtttt tgcccgacgg tatgtcgctt taagccgctt cggcaggctg atgaacagcg
     8221 ccgaactttt gctgttgccg cccacatagc tgactttacc tgtcccgctg tgcagcgctc
     8281 cggccagata atatttttca ttctgtcccg gcgtgaccac ccgtttttgc tgtccgcgca
     8341 gttgccagtc cgcaccgatt ttgggatgaa gatggatatc cacttcatct tcataaaaga
     8401 ccggatgctc tgcgctgcat tcgtccagtg ctttatggat tgctgccatc ttttcatctt
     8461 tatgcgggtc acggatacgc agagttggcg cagcccttcg ccacacaatc cccgcagacg
     8521 gcaaccagcg gcgaacggtt ccggcattta actggcaacc ggttatctca ttgattttta
     8581 ttgccagcag ttctgtactc cagcgtgaac gctggtagcc aaagtcgccg ggagaatgtt
     8641 ttaccagctc acgtaacagt gtgcagatat gctcaaacgg ccagcgacgg gcacgcccgg
     8701 caggtaatga tttcagtccc tcaacacccg actgcgtgaa ccagttaatc cagcgtccaa
     8761 ccgaggaacg ggcgcagcag agcgttctgg caacgtcgct gacacggtcg ccccggtgca
     8821 gcatcagcat ggcagtcagt ctgcgggcat aatttttatc gtgtgtttta tggatggctt
     8881 tctgcatcag gcgtcgttcg tcacgggaaa ttggtgctat gatcggcatt gctcagtccg
     8941 gttggtgatt tgttttgatt tggcgattga tcagatcgca caatccgggc taagttccct
     9001 caaagggatc tactattccg cgcagctat
//
LOCUS       DAJTYG010000126         9028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTYG010000126 DAJTYG010000000
VERSION     DAJTYG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.126"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO568_003450"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO568_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6642661.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1860)
                     /gene="phoA"
                     /locus_tag="OO568_003451"
                     /pseudo
     CDS             complement(446..1860)
                     /gene="phoA"
                     /locus_tag="OO568_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO568_003452"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO568_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6642662.1"
                     /translation="MKNLITELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(2403..2606)
                     /locus_tag="OO568_003453"
     CDS             complement(2403..2606)
                     /locus_tag="OO568_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642663.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /gene="ddlA"
                     /locus_tag="OO568_003454"
     CDS             2684..3778
                     /gene="ddlA"
                     /locus_tag="OO568_003454"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6642664.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGRPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASATCMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGELVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITHLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="OO568_003455"
     CDS             complement(3802..4014)
                     /locus_tag="OO568_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6642665.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..4582
                     /locus_tag="OO568_003456"
     CDS             4274..4582
                     /locus_tag="OO568_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6642666.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO568_003457"
     CDS             complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO568_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6642667.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGSYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO568_003458"
     CDS             complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO568_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6642668.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQGVTHTLS"
     gene            7320..8477
                     /gene="ampH"
                     /locus_tag="OO568_003459"
     CDS             7320..8477
                     /gene="ampH"
                     /locus_tag="OO568_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6642669.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8478..8981)
                     /locus_tag="OO568_003460"
     CDS             complement(8478..8981)
                     /locus_tag="OO568_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6642670.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
BASE COUNT         2233 a         2315 c         2150 g         2330 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cggttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca aggattcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctattgaaaa gattaacttt ttactgacga ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagtaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttaccagcga aacgtttctc ttatttaaat aattacccgc acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcggccgtt gccgacggtg gatgtcattt tcccgattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc aacctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gctcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacattt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca
     4561 ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct
     4621 ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     4681 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     4741 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     4801 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     4861 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     4921 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     4981 ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca
     5041 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagaacca
     5101 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     5161 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     5221 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     5281 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     5341 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     5401 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     5461 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc
     5521 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     5581 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     5641 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     5701 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     5761 tgggttactc cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     5821 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     5881 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     5941 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     6001 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     6061 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     6121 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     6181 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     6241 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     6301 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     6361 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     6421 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     6481 acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     6541 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     6601 agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact
     6661 tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc
     6721 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     6781 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     6841 ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg
     6901 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     6961 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     7021 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     7081 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     7141 acataagcgg attaacacgc gcaggatcac aaatgcaggc agtggtgagc aggaaaaaga
     7201 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     7261 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     7321 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     7381 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     7441 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     7501 gcgtctttcg tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     7561 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     7621 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     7681 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     7741 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     7801 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     7861 cggcgtactc taaccttgcg ttcgacctac tggcagatgc actggcgaat gcgtccggca
     7921 agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca
     7981 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     8041 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     8101 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     8161 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     8221 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     8281 gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     8341 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     8401 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     8461 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     8521 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     8581 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     8641 tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat
     8701 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     8761 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     8821 agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     8881 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     8941 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     9001 atgcccgtca aatccttgac gggcatta
//
LOCUS       DAJTYG010000127         9012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTYG010000127 DAJTYG010000000
VERSION     DAJTYG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.127"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO568_003461"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO568_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6642671.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO568_003462"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO568_003462"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6642672.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO568_003463"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO568_003463"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6642673.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO568_003464"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO568_003464"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6642674.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="OO568_003465"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="OO568_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6642675.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNIIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /locus_tag="OO568_003466"
     CDS             5545..6018
                     /locus_tag="OO568_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6642676.1"
                     /translation="MFDVLMYLFETYIHTEAKLRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="OO568_003467"
     CDS             6047..6589
                     /locus_tag="OO568_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR6642677.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="OO568_003468"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="OO568_003468"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6642678.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="OO568_003469"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="OO568_003469"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6642679.1"
                     /translation="METYAVFGNPIAHSKSPLIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="OO568_003470"
     CDS             7986..8243
                     /locus_tag="OO568_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6642680.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="OO568_003471"
     CDS             complement(8219..8773)
                     /locus_tag="OO568_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6642681.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2258 a         2189 c         2246 g         2319 t
ORIGIN      
        1 ctaacaaata ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaacg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaac gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gtcgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc taggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatgacaggg
     4441 agataaggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taatatcatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctaagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatggta aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc actcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgctttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg
     8941 ggaaccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag ag
//
LOCUS       DAJTYG010000128         8987 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTYG010000128 DAJTYG010000000
VERSION     DAJTYG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.128"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="OO568_003472"
     CDS             complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="OO568_003472"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6642682.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKLWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="OO568_003473"
     CDS             complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="OO568_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6642683.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4606..5763
                     /gene="mdoC"
                     /locus_tag="OO568_003474"
     CDS             4606..5763
                     /gene="mdoC"
                     /locus_tag="OO568_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6642684.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5771..7252)
                     /gene="clsC"
                     /locus_tag="OO568_003475"
                     /pseudo
     CDS             complement(5771..7252)
                     /gene="clsC"
                     /locus_tag="OO568_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
     gene            complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="OO568_003476"
     CDS             complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="OO568_003476"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6642685.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7822..8133)
                     /locus_tag="OO568_003477"
                     /pseudo
     CDS             complement(7822..8133)
                     /locus_tag="OO568_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="OO568_003478"
     CDS             complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="OO568_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR6642686.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQFCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8645..>8987)
                     /gene="csgA"
                     /locus_tag="OO568_003479"
     CDS             complement(8645..>8987)
                     /gene="csgA"
                     /locus_tag="OO568_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR6642687.1"
                     /translation="SGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVG
                     QGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGATVDQTASNSSVNVTQV
                     GFGNNATAHQY"
BASE COUNT         2250 a         2326 c         2133 g         2278 t
ORIGIN      
        1 aacgccggta tttttattgg ctcgttgttt ttgccggatg tggcgtgaac gccttatccg
       61 acctacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg ataagcgtag
      121 cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga
      181 ttgagcttta tcccttcgta ataactcacc catgaagaat atctttcagg ggaattccag
      241 acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg
      301 cgattcagct tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc acgggcgatt
      361 tccagcacct tactggcgcg gtgacgcgct gtcgccattg cggttgccag agcgttaaat
      421 gacgggttaa acactgcgtg cataaagcca tcgtcaaggg agcgttggcg attcatctca
      481 aggaaccgat cggtatcaac cagcacctgc ggcggtgaat actcttccgg gatcaggaac
      541 agtttccaga gtttggtacg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac
      601 ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccagc
      661 cacgccatcc caacagccca cactaacccc aacagcagct gtgagccgtg gcgtttgaac
      721 gcttcgcccc atgaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag
      781 ccaaggaacg cgctgacaac gaagaccgta tggaacagca tgcgtaccgg agccagcagc
      841 acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccgtattct
      901 ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc
      961 atggtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac
     1021 aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgcactac ctgcaatgca
     1081 gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc
     1141 aggaacaccg cacggtgaac cgggtgcata cccttaacca ggaacagacg gaagttcatc
     1201 aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggcaat
     1261 tcttcataag aacccgggag atcgtaagca atccagactc cccaacctgc acggcgcatc
     1321 aacgccgctt ctacgaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc
     1381 agcggagcca gtgcgcagtg ctcgataaat ggtttcacgc ggataatcgc gttatgtccc
     1441 cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac
     1501 acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga cgctttcggc
     1561 gacgactgaa tgatcccggc gttcgggttg gcttccatca agcgcaccag gccgcataaa
     1621 caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc
     1681 cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg
     1741 cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc
     1801 tctgcgacgc agatatccgg gttataactg tcactcagaa tgtagacatc aaagtgtttg
     1861 gcattcccgg tggcttttac tgattcccac gttgcacgca agccagcaaa aacacggttc
     1921 acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca
     1981 tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag ttgcaggaag
     2041 cccatcaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc aaaaaggatc
     2101 aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga
     2161 ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata
     2221 taccaggtcg cgacaacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg
     2281 gtaccaacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga
     2341 tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta
     2401 cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt aagctgatcg
     2461 cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc
     2521 agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt gcggtgttcg
     2581 gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgctgc tttctcgctt
     2641 gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta
     2701 gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtggt
     2761 tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt
     2821 atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac
     2881 cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat
     2941 agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt
     3001 ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat
     3061 ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc
     3121 gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa cgctgccttt
     3181 gccccactcc cctttcggag tcacccatgc gcttggacgg agatcgtaac gatcatcgag
     3241 atcttcaaag cgggagaaat cacgacctcg ctgcaacaga ccaaagcctc gcgggttttc
     3301 catggagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc
     3361 gccattacca gcatggatag acagaccgtt agagtcgtgc aactccggac gatagttatt
     3421 tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag
     3481 tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc
     3541 tggcataacc acgaatttgt aagcacctgt cgcacgcggc gagtcaagca atgcataaat
     3601 ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa
     3661 ttcttcaccc gatggcaaag cggtatcaat tgctaggccg cgggcagaaa ggccataaac
     3721 ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt
     3781 tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac
     3841 cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg
     3901 tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg
     3961 atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt
     4021 aaactggatc tgctgatagt ccgcgtattt catgtcgcgg aaaacggagg gcaagttgct
     4081 tttaggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat
     4141 actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg
     4201 cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct
     4261 tatatcgatc cgatctattt taatggataa ccagccattc cgacaactga atcccggtat
     4321 cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc
     4381 ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa
     4441 ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaacct
     4501 tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata
     4561 acccgacagg gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc
     4621 gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct
     4681 tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca
     4741 ttgtggctga ccctttttaa tgacttcatc cactcgttcc gcatgcaggt atttttcgtt
     4801 atatccggat acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa
     4861 gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccctact gacattaccg
     4921 caatttatta tgctgcaata tgtcaaaggg aaagcggaaa gttggcctgg gctatcattg
     4981 tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt tttactggtg
     5041 ttagtagtca tgacgacgct gtgtgtatgg atatttaagc gcatcagaaa taatttagaa
     5101 aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttatgc
     5161 ctcggcatcg gctatgcggt aataagaaga acgattttta ttgtgtatcc acccattctg
     5221 agtaatggca tgttcaattt tattgtcatg caaacgctat tttatttacc gttctttatc
     5281 ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt
     5341 ggctgtaccc tcgctgcagc attggcgttt gtcgcttatt tactcaacca gcgctatggc
     5401 agtggcgatg cctggatgta cgaaaccgag tcggtgatca ccatggtcct cggcctgtgg
     5461 atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg
     5521 acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt aacgctgttt
     5581 ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg
     5641 atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat cccgttactg
     5701 aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt
     5761 taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg accataaccc
     5821 gcttccagaa actggtggcg gggtcttttt tgagaataac ctctttcttt gcatgacgat
     5881 caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca tcgtactggc
     5941 tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca
     6001 tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata aacaccgttt
     6061 taccatcgat gctaaaagtt ttagcatgca ggctggcacc ggaattaccg gttatgccgc
     6121 gatcgtgtaa cgtactgctt tgttcacgcg tcggcttgag ttcatataat tccacgccat
     6181 agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca acatcgttag
     6241 cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt
     6301 gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg
     6361 gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct
     6421 cccctttcgc cggatcatca cttaataaac gtgtcttcgc ccagataagc ggcaacgttc
     6481 catcaatcag atgatttata aatggactgg attccatttt gcgtaaataa cgatgcgtca
     6541 tggcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca ccctccggga
     6601 catccagcac ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat
     6661 cggcaacgtc ctctaccacg gggcctattg ccatgacatc taaatccgaa aaaagtcgct
     6721 cttcccctgc accaaaatag gcatcaccaa tatttcgtcc tcccactagg gtaaccacgc
     6781 catcgacagt gaaactttta ttgtgcatcc ggcgattgag acgggaaaag tcggtgatat
     6841 atccaagcgg acgtagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcgtg
     6901 gatgactgtc aagcaagcgt aaaatgtcgt caagtccggg cgtattgttg tcgtccagca
     6961 acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgcc
     7021 ccgacatatc gtcctaccag atgtaatact gaacatccag cgtatgctct gccatttcgg
     7081 ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa agaccacact
     7141 gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca
     7201 tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca
     7261 aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt tttaactgcg
     7321 atttcagccg ctgccgcacg agggtaacca taaaccccag tactgattgc aggaaaagcc
     7381 actgacgtat agctgtttgc cgccaccagt cgtaggctat tcagataggc atcctgcaaa
     7441 agctggtctt cgttttgttc accaccacgc cagactggcc cgacggtgtg tactacggct
     7501 ttagcgggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc
     7561 tgttgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc
     7621 ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg
     7681 gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac
     7741 accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag
     7801 caacgtgagt tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg
     7861 tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttgacat tgccgctcat
     7921 taacgctttg ctggagtagg ttttgcccgg aatagaacca ttctatgggc agttcatttc
     7981 tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaaatg
     8041 gatcacgccc ccttgcgcgg tgcttcctgc aatggcaata aaaggaaaaa acaaactgcc
     8101 gagcataaga gagtctaaaa aaggtctgaa catttattct atttcctcaa tgattagtca
     8161 tccttgaggg ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct
     8221 ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag agggcgtcca
     8281 ttgttgtgat aaatgaagtg cctgtccatc agaaacagta acaactattt tcacccgatc
     8341 ttccggggaa atatttaaac tcaacttcgt caaagcaatg ggttgattag caggcaatga
     8401 gagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca gggacaatat
     8461 ttgtactctg cacagacaaa attgagtaag agtaacttca ggaataatgg tgtacatatc
     8521 cccttgctgg gtcgtattaa aggttatctg gctggaaagt gccgcaagga gtaataacgc
     8581 attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta atacaaatga
     8641 tgtattaata ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc acgttgacgg
     8701 aggagttcga tgcagtctgg tcaactgtag caccgttgcc accaccgaac tgtttaacag
     8761 tcatttcaga atttttgccg ttccactgat caagagtggc gctgttaccg aagccacgtt
     8821 gggtcagatc gattgagctg tcatctgagc cctgaccaac atctgcacca ttaccaccgc
     8881 catgctgggt aatagtcaag tcagagttac gggcatcagt ttgcagagca agtgcagagt
     8941 taccgccacc gtactggtaa atgttcagct ctgaatttgg gccgcta
//
LOCUS       DAJTYG010000129         8896 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTYG010000129 DAJTYG010000000
VERSION     DAJTYG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.129"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..2887
                     /locus_tag="OO568_003480"
                     /pseudo
     CDS             1..2887
                     /locus_tag="OO568_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="OO568_003481"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="OO568_003481"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR6642688.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="OO568_003482"
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="OO568_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6642689.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="OO568_003483"
                     /pseudo
     CDS             complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="OO568_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7280..8042)
                     /locus_tag="OO568_003484"
                     /pseudo
     CDS             complement(7280..8042)
                     /locus_tag="OO568_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
     gene            complement(8039..8698)
                     /locus_tag="OO568_003485"
     CDS             complement(8039..8698)
                     /locus_tag="OO568_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6642690.1"
                     /translation="MSEQITFATSDFASNPELRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2168 a         2357 c         2324 g         2047 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgtgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtggtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc gggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggatg
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccagcaggc gcaggcgcag ctgccagaag caattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt gtcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtggaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gtttccttgc agtgtcatga agtgctgcca gagcgagttt ctcttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacaggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagcgccg ctaccctcgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcgccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtacagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aataaagtcg ctgacgaacg gtcagcacaa tatccgtggc gaggatatca
     7801 ttaagcccca attccccacc ctgcactttt tgcgacatat aagccatcgc ttcattgacg
     7861 gcgagcatcg cgccttcgcc cccctgcgaa acgctacctg cgccatcagc aaggaacatc
     7921 accagtaatg gctgctggtc atttagccag gcaacctgca tctggcaggc atcctggcaa
     7981 ggcaagtccg cgctgatgtg cgacgttcca acagcggatg cataaaccag acgccagctc
     8041 acactgacgt ccagcctttt ggcgcttcca gcacaacttc cgttcccggc gtggagcggg
     8101 aaaccgaacg cagcgaactg gataaccagc taaacagttc acggaattgt aatccttgca
     8161 gaggcaaagg ctgacgaacg ctgatttgtg ccagagtctt catatccgcc ccctgaacac
     8221 cgatggaaaa gaaagcaaac cttttatctt cttccccccg aaacactttg ttggctgcag
     8281 cctgccactc atcggttggt gcaccatcgg taatcaggaa aatccacggg cgataatacg
     8341 aaataccatt tgcacgatac tcacgtttcc gctcctctac catatccagg gctttggtaa
     8401 tggccgcacc cattggtgta tcgccctggg caaacaggat gggcggaaaa aaattagcgg
     8461 cgctggtaaa gggttgttcc acatgcaccg ggccgaacgt cacaatgccg agttccactc
     8521 tttttaaagc cagcgaatcg gcaagcagct catcgcgaaa ggtaaccaat ccggtgttaa
     8581 gttcattaat aggtctgcca ctcatcgagc cagaaacatc cagcagcaga atgcaggggc
     8641 aacgtagttc cgggttactg gcaaaatcgc tggtggcaaa cgtgatttgt tcgctcataa
     8701 gaaatccttt cttagagaat tattgaggtc cggtatcata ccgactgcaa taatgaaata
     8761 agagcaaacg aagaaacttt cagaattagt cccggcggcg cagaaaattt tgctattcgc
     8821 ctcgcaagaa cggagagccg tcgattgaca ctaagggcgg agtgacataa tttcaggagt
     8881 gagggttagg gagagg
//
LOCUS       DAJTYG010000130         8667 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTYG010000130 DAJTYG010000000
VERSION     DAJTYG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.130"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..316)
                     /locus_tag="OO568_003486"
     CDS             complement(<1..316)
                     /locus_tag="OO568_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6642691.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVALLWVNGE
                     NFR"
     gene            complement(326..874)
                     /locus_tag="OO568_003487"
     CDS             complement(326..874)
                     /locus_tag="OO568_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6642692.1"
                     /translation="MGLPPLSKTPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYTEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(874..1581)
                     /locus_tag="OO568_003488"
     CDS             complement(874..1581)
                     /locus_tag="OO568_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6642693.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1596..2273)
                     /gene="ydjY"
                     /locus_tag="OO568_003489"
     CDS             complement(1596..2273)
                     /gene="ydjY"
                     /locus_tag="OO568_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6642694.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2278..2988)
                     /locus_tag="OO568_003490"
     CDS             complement(2278..2988)
                     /locus_tag="OO568_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6642695.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYNLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3155..3961)
                     /gene="xthA"
                     /locus_tag="OO568_003491"
     CDS             complement(3155..3961)
                     /gene="xthA"
                     /locus_tag="OO568_003491"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6642696.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGY
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4407..4985
                     /locus_tag="OO568_003492"
     CDS             4407..4985
                     /locus_tag="OO568_003492"
                     /inference="COORDINATES: protein motif:HMM:NF012428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6642697.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGSIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDATCAVIVEPI"
     gene            5001..5627
                     /locus_tag="OO568_003493"
     CDS             5001..5627
                     /locus_tag="OO568_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6642698.1"
                     /translation="MPASNAFLQGLRELCDRHNALLIFDEVQTGVGRTGELYAYMHYG
                     VTPDLLTTAKALGGGFPVGALLATEECARVMTVGTHGTTYGGNPLASAVAGKVLELIN
                     TPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRGLGLLIGCVLNADYAGQAKQISLEA
                     AKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDRFAAACEHFVSRGSS"
     gene            5624..6457
                     /gene="astA"
                     /locus_tag="OO568_003494"
                     /pseudo
     CDS             5624..6457
                     /gene="astA"
                     /locus_tag="OO568_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
     gene            6453..7382
                     /locus_tag="OO568_003495"
                     /pseudo
     CDS             6453..7382
                     /locus_tag="OO568_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            7379..>8667
                     /gene="astB"
                     /locus_tag="OO568_003496"
     CDS             7379..>8667
                     /gene="astB"
                     /locus_tag="OO568_003496"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR6642699.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQSVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQ"
BASE COUNT         2020 a         2226 c         2382 g         2039 t
ORIGIN      
        1 tgcggaagtt ttcgccgttc acccagagca gagccaccga gccgcccgtt ttacgaccgg
       61 cagcagcttc ggtctgaatg cgcttcaccg cgtctgcggc atccgcgaga cgaacaatct
      121 tcaggtttat agcgtaatgg gttttcatct cgccgctaac ccagtcgaga tagcggttaa
      181 ttgcggtatc gccgccccag gcgttaaacc agacggtttg ccctttggcc tcatttttaa
      241 tttcctgcca gtcactggca tgtgttgcca aagaaaacag cgataacaat cccagcaacc
      301 acccacaatg gcgcatcttc cctccttaag aacgtttttc cggaatacgg cacagcccct
      361 gagcgggaat gtccatggca gaattaaaac gagccgacag gttttgcagg ccgatcaatg
      421 ccgtcagttc ggtgagcgcc tgagcgtcaa aatgcgcagc cagtcgggta cgcagggcat
      481 catcgactgt tggcggtgtg acgcttgcgg cttcggtgta ctccagcgcc agccgttctt
      541 catcgctaaa gagcgggctt tgccgccagt cagccactgc cagcagttta tcggtgctgc
      601 cggtacgctc ggcgactttc atactggtga tatccacgca aaactcacac aggcacattt
      661 gcgcaatgcg cgcgctgaca agcgatcgca ctaccggatc gagcggtgag cgtttgcgct
      721 ccagccaccc aacaaacatt gacaccaaat aaaagataaa cgggatccgc ccccaccagc
      781 gaatcgggct tagcacctcg ccgtaatggc gacgatgcag ccacgcctgc ggacgtaaaa
      841 tgaaaggagt tttgctaagc ggcggtaaac ccatcagtca ttcctcttct ggcgctcaag
      901 ccatattttc ttcgccataa aaatcaccac ggtcagggca aacagaataa acagttcggt
      961 gacaaaccag aaagtaccgc ctgttaacat gctgcccgcc caggaataaa caatagtcgc
     1021 cggtaattga ccaagcccgg tggcgataaa aaacgagcga aaacgtattg aagtcagacc
     1081 ggcagcatag ctgattggat cgaaagggac aaaaggcaat aaccgacaga ccaggatggt
     1141 gtgtttgccg tagcgagtga aaaagccgtc catactgtca agcacggttt tgccggttaa
     1201 tttttccacc acttcgcggc ccatcactct ggcgataaaa aagcacagcg ccgcgccggc
     1261 catcgaactg gtccacgaca gcaagccgcc ccagaacgcg ccaaacagcg acgcattggc
     1321 aaaggtgatc aaaaacgcag gtagcggtgc agcaatggcc tgtaaaatca tcaacaagaa
     1381 cgagacaact gccgccagtg cgccgtaaga ctgaataaag cgttctatac cctgttggtc
     1441 cacggcggca aacgccgcaa cgctgcgatt gataaactca tggacgccgg gaagcagcgc
     1501 ccatgcgagc agcatagcga acaacaggat gatgagggtt atacggtagt accagatttt
     1561 acgcgattgt atcttcatca tcctttatcc tggctttatt cggtgatttt aaaggtaacc
     1621 gttgccagcg tgttatccgc cgggagaact gaggcattgc ctttgaattt cacttcgccg
     1681 cgtttttcca ccgcaccata agtgtatgtt gcattgctga cgatgccgac cgggcagcta
     1741 tccagacaca ccaggcaacc cgttttcttc tcttctgctg ccgttaaatt accgccaaag
     1801 cgcatgtcca gtttcttgcc attactgtca acaatcactt catcgaagga atacgctttt
     1861 gccgcccctt gccagttgac cgaaatatcc agtttgctgc ctgtgacatg agtcgtttct
     1921 ttattatcca tcgtcatgtt ttcgcccggc gtaccacctg cctctttcag ggcttcataa
     1981 aatgctttcg gggtcgcata agccataaac agcgatttat gtccgttgga gccatcttta
     2041 aacacaatac cgtgacgagt gtcgtcggtg aggaaacgac cattaatttg caccagcatg
     2101 gttaccgtat ggttattggc atcgacgcgc agcggttggc tgttcgaaag tccgtcgtat
     2161 tccacttttg ccgccgcatt ctctttttga tcgcagccgc taagcccggc aacagccagt
     2221 aagcagagtg cagccagtcc ttttttccat gacactgaat aatgttgcga catcgttcta
     2281 tccttcattt tttgggtgag taagtgggct gcggcgcgaa gcagacagat ccacatgttt
     2341 gtgtcgggcg tagagttttg cgagctggat gagaataaac agcgccaggc ccgccagaca
     2401 gagttgtaaa ataaagcgca gcgtaatgcc ttcattggcg agatcgcttg ccatcacggt
     2461 ataaataaca ataccgggca gggtcgtgag tgccgaaata agggtataag gccagaaggc
     2521 gatggtggtt aatccgtaag cgtaattttg aatattgtaa ggaaacaacg ggattaagcg
     2581 ggtcagaata agaaaatcaa taccgttacg cgcaatgcct ttttcaatgg cctggaaggt
     2641 atggctatgg cccacgtatt tcagcagtaa atcacgtccc agccagcgcg ccagcaggaa
     2701 tgagcacgag gaggccagcg ttgcggcaat taatgagagt agtgtcccta agagcgggcc
     2761 aaaaactatt cctccagcga tcaccagtat gcttcctggt aacagaaaga ggggggcatt
     2821 gatgaataac agaatataga ggttataacc gaacaatccg ctctggcgaa tgagtgtctg
     2881 taagtggggt aaatcggtga gcagatcgaa taaaccgaaa gcgtggatag cgtaaatgac
     2941 cagcgcaaaa ataagacagg caaacagaaa gttacgctca gcgttcatcg tggagtttcc
     3001 tatatcaaac ttgataatag agaagtggtc agacaactaa aaacgcagtg acggtataac
     3061 aaaggacggc aggcaacaaa tcattactga cagtttgttg atttttctca aggttaattc
     3121 tcctgaccca gtttgagcca ggagagctgc taaattagcg gcggaaggtc gcccagacgg
     3181 gggcgtgatc ggacggtttt tccatgctgc gaatttcata gtcgatgccg gtttctacgc
     3241 aacattctgc cagcggttgg ctggcgagca gcaggtcgat gcgcagacca cggttatcgt
     3301 caaaaccttt tgagcggtaa tcaaaccatg agaaacgatc tgctgtttgc ggattagcat
     3361 ggcggaaggt atcgaccacc ccccagctca tcagcctgtc catccattcg cgctcttccg
     3421 gcaggaaaga gcatttaccg gtacgcagcc agcgcttacg gttttcttcg ccaatgccga
     3481 tatccagatc tgtagggctg atattcatat cgcccataat cagtaccgga ttatcacgtt
     3541 tgagttcggt ttccaggtag ttttgcagat tctgataaaa ctgcgctttt gccgggaatt
     3601 ttatcggatg gtcgcggctt tcaccctgcg ggaagtaacc gttgatcacg gtgacataac
     3661 ccagcagtga ggggatttcc gccataataa tccgccgctg cgcctcttcg tcgtcaccgg
     3721 gaaagccgcg acgcacggca atcggcgtct ctttggtcag cagcgccacg ccataatggc
     3781 ctttctgccc gtgataaaac acgttgtagc cgagcttcgc cacctcttcg agcggaaaca
     3841 tatcgtcatg aacttttgtc tcctgcaggc caatcacatc cggttggtgc ttttcgacga
     3901 tggcttcaag ctggtgaggt ctggcgcgca ggccgttgat attaaaagag acaaatttca
     3961 tagtcgctgc catttattca gataaagagt gcgtggatgg tagcagaatt tcgcgttgct
     4021 taccgcctgt tgttaagtga tttgatcgat ggttgttaat gatgtcaacg atggcgcaaa
     4081 aaatgcccgc ttttggtgcg cgctgcgtca gaatggcgca gtaatttcca gtaaatttcg
     4141 tcaatgatca caattccaga attatatttt acttctgcat aacattgcgt tttttattct
     4201 ttttattcac ccacatcgca aacgtattca ctttatatgc actttaaatg catatggttt
     4261 ggttataact ccttgatttc cagttagcct ccgccgttta tgcactttta tcactggctg
     4321 acacgaaccc tgcaatctac atttacagcg caaacattac ttattgttaa catacaaata
     4381 acgaattatt tactgtagag gtcgctatgt ctcagccaat tacgcgtgaa aactttgatg
     4441 aatggatgat acctgtttac gctccggcac cctttatacc ggtacgtggc gaaggttcgc
     4501 gcttgtggga tcagcagggg aaagagtata tcgacttcgc gggtagcatt gcggtgaacg
     4561 cgctgggcca tgcgcatccg gaactgcgtg aagcgctgaa cgaacaggcg agtaagttct
     4621 ggcataccgg caacggttac accaacgagc cggtactgcg actggcgaaa aaattgatcg
     4681 acgccacgtt tgccgatcgc gtcttctttt gtaactccgg tgcggaagcc aacgaagcgg
     4741 cgctaaaact ggcgcgtaaa ttcgctcacg accgctacgg cagccataag agtggcatcg
     4801 tggcgttcaa aaatgcgttt catggtcgca cgctgtttac tgtcagtgcg ggtgggcagc
     4861 cagcctattc acaggatttt gcgccactgc cggcggatat tcgtcatgct gcatataacg
     4921 atattaactc tgccagcgcg ctgattgacg acgccacctg tgcggtgatt gtcgagccca
     4981 tctagggcga aggcggtgtg gtgccagcca gcaacgcgtt tttacaaggt ctgcgtgaat
     5041 tgtgtgaccg ccacaatgcc ttgctgattt ttgatgaggt acaaaccggc gtcgggcgca
     5101 ctggtgagct gtatgcctat atgcactacg gcgtgacgcc cgatctgtta accaccgcca
     5161 aagcgctggg cggcggtttc ccggtcggtg cgttgttggc aaccgaagag tgcgcccgcg
     5221 tgatgaccgt tggcactcat ggcaccacct atggcggtaa tccgctggcc tcggcggtag
     5281 caggcaaagt gctggagctc atcaacacac cagagatgct taatggcgtt aaacagcgtc
     5341 acgactggtt tgttgagcgt cttaatacta ttaatcaccg ctatggtttg ttcagtgaag
     5401 ttcgcggctt aggtttgttg attggctgtg tactgaatgc cgattacgcc gggcaagcga
     5461 aacagatctc tctggaagcg gcgaaagcag gcgtgatggt actgattgcg ggtggcaacg
     5521 tggtgcgttt tgcgcctgcg ctcaatgtca gcgaagaaga ggtgacgacc ggactggatc
     5581 gctttgcagc tgcttgcgaa cactttgtta gccgaggttc atcatgatgg tcatccgtcc
     5641 cgttgagcgc tcagatgtct cggcgctgat gcagcttgct agcaaaacgg gcggcggcct
     5701 gacgtcgctt cccgccaatg aagccacgct ttcggcgcgt atcgaaaggg caatcaaaac
     5761 ctggcaaggc gaactgccta aaagcgagca gggctatgtg ttcgtgctgg aagatagcga
     5821 gacaggcacc gtggcgggga tttgcgctat tgaggtggcg gttgggctga acgatccctg
     5881 gtacaactat cgcgtcggca cgttggttca cgcctcaaaa gagctgaatg tctataacgc
     5941 attgccgacg ctgtttctta gtaacgatca caccggcagc agcgagctgt gcacgctgtt
     6001 tctcgacccg gactggcgca aagagggcaa cggctatttg ctgtcgaaat cgcgctttat
     6061 gtttatggcg gcttttcgcg acaagtttaa tgacaaagtg gttgctgaaa tgcgcggggt
     6121 gattgacgaa cacggctatt caccgttctg gcaaagcctc ggtaaacgct tcttttcgat
     6181 ggattttagc cgcgccgatt ttctctgcgg caccgggcaa aaggcattta ttgcagaact
     6241 gatgccgaaa catccgatct atacccactt tttatcccag gaagcccagg acgtcatcgg
     6301 tcaggtacat ccgcaaaccg cgcctgcccg cgcggtgctg gagaaagaag gttttcgtta
     6361 ccgcaactat atcgacatct tcgacggcgg gccaacgctt gagtgtgaca tcgaccgcgt
     6421 gcgcgccatc cgtaaaagtc ggctggtgga agtggcagca ggctggcttg ccgccgggcg
     6481 tgctgaacct ggtgcagggc gggcgtgaaa cgggtcaggc gctgagtgcg ctggaggatc
     6541 tcgacggttt gctgtttacc ggtagcgcca ataccggcta ccagctgcat cgccagctct
     6601 ccggtcagcc ggagaaaatt ctcgcccttg agatgggcgg taataatccg ctaattatcg
     6661 atgaggtggc ggatatcgac gcggctgtcc atctgaccat tcagtcggcg tttgtcacag
     6721 ccgggcaacg ctgcacctgc gcccgccgtt tattgctcaa aagcggagcg cagggcgatg
     6781 cgtttcttgc tcgtctggtt gccgtcagcc agcgattaac gccgggcaac tgggatgacg
     6841 aaccgcagcc gtttattggc gggctgattt ctgaacaggc cgcacagcag gtggttactg
     6901 cctggcagca actggaagcg atgggcggac gaaccctgct tgcgccgcgc ttattacaag
     6961 cagggacatc gttgctgaca ccagggatca ttgaaatgac gggcgttggc ggtgtgccgg
     7021 atgaagaggt gttcgggccg cttttgcgcg tctggcgtta tgatactttt gatgaggcga
     7081 ttcggatggc gaacaacact cgcttcggac tcttttgcgg tctggtttcc cccgagcggg
     7141 aaaaattcga tcaactgttg ctggaggcgc gggcggggat tgttaactgg aacaaaccgc
     7201 ttactggtgc tgccagtacc gcgccattcg gcggcattgg tgcttccggt aaccatcgcc
     7261 ccagcgcctg gtatgccgca gattactgcg catggcctat ggcgagcctg gagtcggact
     7321 cgttaacatt gcccgccacg cttaaccccg ggctggattt ttccgatgag gtggtgcgat
     7381 gaacgcctgg gaagttaatt tcgacgggct ggtagggctg acgcatcatt acgcgggcct
     7441 gtcgtttggt aatgaagcct ctacccgtca ccgttttcag gtgtctaacc cgcgactggc
     7501 ggcaaaacag ggcttactga aaatgaaagc ccttgccgat gcgggattcc cccaggccgt
     7561 gatcccgccg cacgagcgtc cgtttattcc ggtgctgcgt cagttgggat tcagtggtag
     7621 cgatgagcag gtactggaaa aagttgcacg ccaggcaccg cactggcttt ccagcgtcag
     7681 ttccgcttcg ccaatgtggg tagccaatgc ggcaacgatc gcgccatctg ccgatacgct
     7741 ggatggcaaa gtgcatctca ccgttgccaa cctgaacaat aaatttcacc gttcgctgga
     7801 agcgcccgtc actgaatcgc tgttaaaagc gatttttaag gacgaagaga aatttagcgt
     7861 ccattcggcg ttgccgcagg tagcgttgct cggtgatgag ggggcggcaa accacaatcg
     7921 tctcggcggt cattacggtg aaccgggtat gctacttttt gtctacgggc gagaagaagg
     7981 caatgatacc cggccttccc gttatccggc gcgacagact cgcgaagcca gcgaggcggt
     8041 ggcaaggctt aatcaggtga atccccaaca ggtgattttc gcccagcaaa acccggacgt
     8101 tatcgaccag agcgtttttc ataatgacgt gattgccgtg agtaaccgcc aggtgctgtt
     8161 ttgccaccaa caggcgttcg ctcgccagtc acagttactg gcaaacctgc gggcgcgggt
     8221 gaacggtttt atggcgatag aagttccggc aactcaggtt tccgtgtcgg atgcggtgtc
     8281 tacctatctg tttaacagcc aactgttgag ccgcgatgat ggttccatga tgttggtgct
     8341 gcctcaggag tgtcgggaac acgccggagt atggggttat ctcaatgaac tccttgccgc
     8401 tgacaacccg attagcgaac taaaagtctt tgacttacgt gaaagcatgg cgaatggcgg
     8461 cggcccggcg tgcctgcggt tgcgggtggt attgacagaa gaagaacgcc gggcggtgaa
     8521 tccggcggtg atgatgaacg ataccttgtt taatgcgctc aatgactggg tggatcgtta
     8581 ctaccgcgat cgtcttactg ctgccgatct ggccgacccg caattgctgc gcgaagggcg
     8641 ggaagcactg gatgtattga gccaatt
//
LOCUS       DAJTYG010000131         8610 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTYG010000131 DAJTYG010000000
VERSION     DAJTYG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.131"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(623..2332)
                     /locus_tag="OO568_003497"
     CDS             complement(623..2332)
                     /locus_tag="OO568_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR6642700.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVANSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2764..3483)
                     /locus_tag="OO568_003498"
     CDS             complement(2764..3483)
                     /locus_tag="OO568_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR6642701.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQCKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3608..4164)
                     /locus_tag="OO568_003499"
                     /pseudo
     CDS             complement(3608..4164)
                     /locus_tag="OO568_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4148..4406
                     /locus_tag="OO568_003500"
                     /pseudo
     CDS             4148..4406
                     /locus_tag="OO568_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4515..5093)
                     /locus_tag="OO568_003501"
     CDS             complement(4515..5093)
                     /locus_tag="OO568_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642702.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(5514..5751)
                     /locus_tag="OO568_003502"
                     /pseudo
     CDS             complement(5514..5751)
                     /locus_tag="OO568_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(5938..6367)
                     /locus_tag="OO568_003503"
                     /pseudo
     CDS             complement(5938..6367)
                     /locus_tag="OO568_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6415..6780)
                     /locus_tag="OO568_003504"
     CDS             complement(6415..6780)
                     /locus_tag="OO568_003504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642703.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDARNKKLVFWFVRVDDEGYPEIARCMEREFATIPAGINADG
                     MYCPECGTVHWPDGVIPPF"
     gene            complement(7533..8006)
                     /locus_tag="OO568_003505"
                     /pseudo
     CDS             complement(7533..8006)
                     /locus_tag="OO568_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(7934..8300)
                     /locus_tag="OO568_003506"
                     /pseudo
     CDS             complement(7934..8300)
                     /locus_tag="OO568_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8302..8610)
                     /locus_tag="OO568_003507"
                     /pseudo
     CDS             complement(8302..8610)
                     /locus_tag="OO568_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2262 a         2077 c         1686 g         2585 t
ORIGIN      
        1 aagcttgata caactggtct ggtatataca gtttctttga gtgcggttac gtccacagag
       61 gtaacggtaa aagtaacccc ttgtcgggac cactccgacc gcgcacagag gcattttcat
      121 cgctgacagg ccgcattgcc tcgcagggct ggaggaggat cggtgatatc cggacgaccg
      181 taccggctta cgccgggctt cggcggtttt actcctctgc catgtgcaac aacggagtgc
      241 cgccttacat gccgctggcg cggcatatcc tggtaacatt tttgttgata ccaccttccg
      301 gccttatcct gaccagtggg ctttcctggc atcgatgata ccgatgaacg cgcatgatat
      361 tgagccgacg atattacggg caactggaaa tactcactct ctggatgtga cctttatcca
      421 tgaagaagat ttggccacac catggaaacc tgaacaatca tcagtttacg cccatgtaaa
      481 cccctacggg atatttgaac tcgatatgga aacaaggttg ccaatcgagg tggttgcctg
      541 aaagttggca gttacccggt cactacacct tgttggagtg aacatatcaa aaacaggtaa
      601 ttacacaatt taaattacag tctcataaaa aatgattaaa tctatttatt atttcaataa
      661 acgttttttt catcttcttt gaaaattgtt ctgctatagt atccggttcc agattgttct
      721 cttttattat tctacaatac tctaatattg tttctttgtc tctatttact acagatacaa
      781 atacaccagg aataccatct ttattagttg cggacagcat ttcgaccaat cgtcttgtgg
      841 taaaactata gttttttaga atattacaaa aggcacgtat tgtttcggta tggccattat
      901 aaaatgccat aaaaagtcct gatgtaccgt tgggattttt tatccataat aattcatcta
      961 tatattcctg gtgagggggg agtattttta gtatatctcc atacgccata atggcctttg
     1021 tgtgcccatt ttgaagtgcc ataaacaacc catgctttac tttcaataat tctttaattt
     1081 gttcactact aagcattttc ttattaagca gacttccgaa catacgaata ctatctgcat
     1141 gtccattctg caatgcaaaa aataacccag gtaaatcatg gctgtctttc gcagctaata
     1201 gatataatct cttatcggaa gttaacgggg ttttaaagac tatttcccca taggctttta
     1261 ttgtgtctac atgtccgttc ataaaagaca tgcataaacc cggcacattg tttgccccta
     1321 ccgcagcaag caatgtaagt attgtttctt gactaagatt gcacatattt aatatcttac
     1381 catatacttg aataatgtct gaatatccgt tttgaagtgc tatataaaat gcaggagtac
     1441 catcagagct tctcgtggcc agaaggttca ccagtttaga ttgagaaatt tcaccatttt
     1501 tgtatttttc catcagtata aataatactt ctgtcagatt ctttgttatc gcatgatata
     1561 taactctgga attgatcaat tcatctggta aaagtttcac tgtgtttgtt agaggggaat
     1621 tatgcctatc ttcaaatatt acctgcccgg gttgctccag accataaatt ttccaataaa
     1681 aatcatcaaa aaaatcctta gcggataatt ggctcagttc gaaatggcta tcacaagtaa
     1741 atacagtacg tttatgagta acagttcgat taggatcgaa aaactgaaca acccattttt
     1801 cactttcttg tacgcgcttt attttcaacc ctaacgccat ttggtggttt aatgtgcatg
     1861 ctgataatgt tctgacaaag tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa
     1921 ctgttcccca ctttttattt tccacaaaat aaatataagc tggtgatttt gaaaaatctt
     1981 ccgttttttc caaacataca ttttttgcaa tagcatcagg ggtgctaaga agctgatagt
     2041 ttaccttccc atttgtttcc ataaaattca tttcccaata ggaagctagg tgacggcaaa
     2101 ctatttgtct tccatatata tcttttgcca ctccatttat atttaccttc acatttctct
     2161 tttctgaaaa ataaggtgct gttttattct taaactgaat tagttcactg gagtttgcga
     2221 ccctcataat agaacttgtt attgttctat tatctctgtt aacagacata tctgttgata
     2281 acgaattttt tgttgaaaat atactagatg aaaatgtttt atttaacggc atagaagaca
     2341 accatagaga tgttataata ctattagatt gcagttacta atagtaaaag cctccttaca
     2401 aggaggtagc ttattcagaa tgctcctata agatagcgag ctcgtcaacg atacagatga
     2461 tctgattata ttgtgcaaaa ggagaacagg aaaataggca ctggaaaaga tgaaggcctg
     2521 atgggagcat taactaatgc aaaatccgac aggttaatat cccgaaaagc aatcaaacga
     2581 ctcaaacagg agatgcacgc tgaagtggtg gacaactgtt cggatttcag atgagtagct
     2641 cagcagttta tgagccgaat gctctgtcac gggcaggaat caggctgcat acgcagtgcc
     2701 aaatctaatc gtagttattc taaccagttt tcaagctaca aggtggtgta gctggcatct
     2761 tctctactct atcatcaaac gataaaatgg ttcctctcgt aacatttgct cttgcctttc
     2821 acttccatct cgatcgcttc gtagttcatt acgatagctg acatatttcc agtcttcagg
     2881 tctaacatct gacgccggat actccccggg agcgattttg cttcttaata gattagactc
     2941 gagacacatt ataaattgtc gtattttatg aagtaataaa gcactatatt gcgagcattc
     3001 ctgatctgat tttacatata gcgtaaactg agctcctatc cccacacgag attgctgaga
     3061 aactcgattc atatcagtta tcttccattt atctatagga ctgtcctctg aaaataataa
     3121 accagacaaa atttggaatg ccagaggaac ttgctcccta gctatactaa tatgaaactt
     3181 gtccccaaca aaatccccct tacactgagg attttcacga catgcatgaa taaacacatc
     3241 tccactatgg ttcacaatct ggaatccttg gtacatgaca gaaaaaaatt gacatatatc
     3301 agagtaagtt ggcacgttcg atgctacttt gaaatagtac gctgggtatt gccgtatttg
     3361 gttgtacaat atattgaagt tatttttcaa tctttcattc gcagaaagca tctgcggtat
     3421 ttcagacctc acaacattca aagaatctaa atttagtttt agacagggct tttttatggg
     3481 catagaaaac gtcctctata aaatagatat attatcttat attgattgtt tatcatcttt
     3541 tataaaacga tacattatat gtaatgtatg taagcgtaag ggtcttctcc gatcatggtg
     3601 ggaaggctta gaacgccata ttcagtttcc catagtggaa catcactccc agcttaaagc
     3661 gctcccggtt gcggtagccc ttggctttta ttctcagcag cctgatcttg ctgttcagtg
     3721 cttccgcatt tccgtttgat acgccatggc gcatggcgtt caggataccg tacaaccttt
     3781 tccctatcgt tttagccaca ttcttcatca tggggacgtc actgttggcc gcagccatct
     3841 ctgccagtcg ttgcgtcttt cctcgctcca tggtctgctc cagatatcct ttgccagttc
     3901 tttcagcgcc cagcacaggc tcgtcagctt catctgcgca cgcagccata tcagcttttc
     3961 ctgcctggat tcggtcatcc atttatcgct gtgctgccac aggaagcgag ttccctttgc
     4021 ctgacgacga ctttcaacag gaagatgcgg atgttcattc tggcgggttt tatccaccac
     4081 ctcacccggt tgtttcgcca catgaaagcg atcgaaggct attttctcaa ccgcattggg
     4141 taagtggatg cgcgctgctc ttagtttgct gagccagttt agcattgcct tctacatgac
     4201 cgatgcctga gccggtatac cgaactttat tgtcctcaac cagtcatgtt ataagcaaga
     4261 aatgcactca gcggatagag cgacataatc tgaacctgcg gacacatctt aagcggctta
     4321 cccgcaaaac catatgcttc tcaaagtctg atgatatgca ctacaagatc atcggctggt
     4381 atctgacaat caaccaccac cactaaatct gcgtcacgac ctgcaactga gtctggttga
     4441 acaggctgag gccctgctga agcaacgcga acctctcgac tcttgttgga ttttaccaaa
     4501 tagtttttct aaaattaaac acatttatat aagttctttg ctgaacaaaa cgcagcccgc
     4561 catccttttt cattcataaa ttcagcaatg gcagataaaa ctttttcatc aagcatataa
     4621 aaaataatgc cagtagactc aatatgcctc atacaaataa ggccgcacat atccgtaata
     4681 tatttgtcaa tgctgctacc atttgcatgg taatatgcca tgctattatc tgatgatttt
     4741 acatatgcag tatgacatat ttttccttca cgattaacaa aactgacaaa accactttca
     4801 ccaggagtaa tatataaaga tgaatttaac gtgcgctcac taatattcat taattgttga
     4861 aattcaatgg aatagttttc agttaatgta tctattatct tggctttgtc tttctttgtt
     4921 atctcattgt ctttttcggc aagaaaaatt gccactttat gacatgcatt cagctcgtta
     4981 attttacctg ataaaactgc ttttcttacc acatcgttga actcatcgat agtgtgtttt
     5041 ttattatata tatcatgaaa aatatagcct aaagacttaa acgagacttt cattttatat
     5101 tctccttaaa tattaacagg atatatatat cacattttcc tctatatttt cagaaaccag
     5161 ctatcatttt ccccattact ggtacgtcgc ttcagatatt tccgtagcaa gcgataaccc
     5221 tgcgcactat gaggatgcca ttcattataa tgtttgaaca ccttcgcgag actactttac
     5281 tgctgttaac acgtcaggtt cgaacataac actgatttaa tcatatttca tcgttttgac
     5341 gaagttttct gccattccat tttgcaccgc cgtgtgtttg ggggcaaggc cgaccatctg
     5401 tgtttcattc gccagatata agtgatgaga tggcattctc cctccttcac ggcagaagca
     5461 ttttgaccgt ttaggggatg cggacgattt tcatgcctgc taagatactt tcgttactga
     5521 tgctgtccgt tttccaccat ttcatcatca ctgattggcg ttgtgttttt ggtcagcact
     5581 ccagctttac gtttctcttt taaccggtaa ctctctcctt tgatattcag cgtgtagcag
     5641 ccgatctagg atcgctgttg ccagcacgtg atctccgaac atttctcccc agtctgcgac
     5701 ccctttgttt gacgtcagta tgatgctcgc tttttcatat cgacagttca tcgataggaa
     5761 tttaaatccc caaaagatta aaaaagcacc gaagaatgcg cgtttattca acacaacctg
     5821 atcatcaagt aatcggtata gacacttaat attgcttaca ttgattgatt caatgtatct
     5881 ctgatggttt tttgctccgt tcccctcaac atggacgcaa cttcccctat gcgactctca
     5941 gccgcaccac cgtattcgag ccagcggctc atgcatcata acctgctctg ccagacggta
     6001 acccctcttc agccccgtaa accgcatctg acctccgcgc agctcgcact tcagcgggtc
     6061 aaccttcagt cactgctgat acatccctct ccttgtgatt tgcctcgccg tttttctcac
     6121 tgtctccgtt atggcgtaca ccacatcttc cagtaatcgc cgcttcaccg gactcaggac
     6181 cccgtatcac catccggaac cccttatccg ccacatgcca ggagaacctt tccataaact
     6241 cgtcgccact catcacaagg ttttcttctc gttttgtccg gtggctgttg taacgcaggc
     6301 caatttcatc ctgaccggca taatgttcca ggcggctcat ctgcaccggc tgcttctcca
     6361 gccagttaag ccatcggttt ccctgctgaa aaatatcggc aaaacgggga agcatcagaa
     6421 aggcggaata acgccatccg gccagtgaac cgtaccgcat tcagggcaat acatgccgtc
     6481 ggcgttgata ccggcaggaa ttgtcgcaaa ttcccgctcc atgcagcggg caatttccgg
     6541 atacccttcg tcatcaacac gtacaaacca gaagaccagt tttttgtttc tggcatccac
     6601 aaagaacgga atattcaggt ctgcgcaaca ctcaacggca tcgtcaaaat tatcaaagcg
     6661 cagaacctca gcttcttctt cgtcaaaaaa atcatcttcg tgaagtttta cgacatagcg
     6721 gaggagtttg cttctttgag gtgcaggttt tcgtttgcgt ggtttagctg aacgggccat
     6781 ataactacct gaaagacaat gatattgtct gtttttataa cggtaatcac aggccatgac
     6841 aagccgcagc cgtcaggccg cctacttggg gcggtttaga acgcttattt agtaccagat
     6901 cttacttata ataaaggggc ttggcagttt ggataaacta ttgtccttat tccactaata
     6961 caaacttttt gtaaagtata ttaaatatac ttcatttcat actgaaatcc ccggaagtat
     7021 tcaatcaaac aattacacca taaaaggaag agggggcccc aattaagttg tcaagcatgt
     7081 ttactgcgca atcgcttggt gctccgtccc tgtaccccgc attggtactc tggcgctcat
     7141 ggggcttcca cttgcgcttc tcccttcaca tcggggtgac aggtccacgt tccccacaag
     7201 agcctgaatc gacttcacgc cgcttttatg ccggatgccg cgcaggcagt aaacatatcc
     7261 cacaggaacg actccaccat tgttttgaca tcatctatat gctttcgaca catcatcagc
     7321 ggttcacttt cgttcgtctc ttcgaaccat acttgatcgg ctcacgtccg accttttccc
     7381 atctcgctca ccaccacatc tcttaaatgc agcagcagtg gatggtttgg tgcctgcgcc
     7441 tgcacgccga caccgagggg ccttccctca tctcttgtgc tgcatggctg cacagggact
     7501 cacgtcccca ggacgcgcct tcgtggcaca cagtcatcgg catagcaaca gtaagcaacg
     7561 gcaggtttcc attgccagtt ttctttgaca gcagtacttc ggcctcgctg gatactgtga
     7621 ttccagtacc agcgatcctt tctggctttt ccactcaggt agcgtttatt cagatactgg
     7681 tcgaactcat tcagtatgat gttcgacagc aacggcgaga tcacaccgcc ctgtggcata
     7741 ctttcactga ccgcccggaa gagaccaaca tcaacacggc gttttccaca gcagtctcat
     7801 aaaacgtgca tcactgatcc tgcggcagac ggctttcatc agcagccggt gatgtactgt
     7861 gtcgaagtag ctggacaggt caccttcaat aacccggcgc cccgtgtttc cccgcagtcg
     7921 gtgagctgta atagtacgtg atgtctcccg cccacttctg gttcgggcca ctggcgtaaa
     7981 aatcctgcgt cagcagattt tctgatacag gcaggccgtg tgcacggtaa ctgatcgggc
     8041 tgaacttctg ggaggctttt gcgctcagtt cctgacggtg cagactggct gccacggttt
     8101 ttacgttaaa gtggtaacca gagcacacag ttcatccgtc aggcgtgggg caccgtaacg
     8161 ctggactggg taaaagcctt gcggacaacg ctgtcgcagt gctgacggaa ctgctgacgt
     8221 gtgtttatcc ttgtccgccg ctgacaccac gtataccagc cgctgcgggc cacccggagt
     8281 acgcggcaca ttgccttgat gacggtttac gcggtcgcct gcggcaacgg ctttccggct
     8341 gacggtagtg tggacctttt taccccggag aacaccggca agccccataa ccgccatgag
     8401 acgtgccaca gtgcatctgg ccactctgat accttcccgt aacaactgat gccagacttt
     8461 acgcacagcg tatacctgat gattctcatc gtatacgcgc agtgtctctt tcttcagcca
     8521 gtcatcgcgc tgcgcacggg cactgcgttt atcatgatga tgtcgctgtt gctgacagtg
     8581 gtaatacgtt gacggggcaa tatgcagttc
//
LOCUS       DAJTYG010000132         8351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTYG010000132 DAJTYG010000000
VERSION     DAJTYG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.132"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..158
                     /locus_tag="OO568_003508"
     CDS             <1..158
                     /locus_tag="OO568_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642704.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(167..565)
                     /gene="vapC"
                     /locus_tag="OO568_003509"
     CDS             complement(167..565)
                     /gene="vapC"
                     /locus_tag="OO568_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR6642705.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(565..792)
                     /gene="vapB"
                     /locus_tag="OO568_003510"
     CDS             complement(565..792)
                     /gene="vapB"
                     /locus_tag="OO568_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR6642706.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            874..1293
                     /locus_tag="OO568_003511"
     CDS             874..1293
                     /locus_tag="OO568_003511"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR6642707.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTGDDGRTVRNGADR"
     gene            1280..6145
                     /gene="traI"
                     /locus_tag="OO568_003512"
     CDS             1280..6145
                     /gene="traI"
                     /locus_tag="OO568_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCR6642708.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQYNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIA
                     PGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQVPR
                     LEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEA
                     REQTLIVTHLNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGELRRLSTWE
                     NNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRV
                     GTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPA
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQA
                     VREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVR
                     DLQKEKTPGGD"
     gene            6165..6911
                     /gene="traX"
                     /locus_tag="OO568_003513"
     CDS             6165..6911
                     /gene="traX"
                     /locus_tag="OO568_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR6642709.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6966..7526
                     /locus_tag="OO568_003514"
     CDS             6966..7526
                     /locus_tag="OO568_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6642710.1"
                     /translation="MIEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7657..7869
                     /locus_tag="OO568_003515"
     CDS             7657..7869
                     /locus_tag="OO568_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6642711.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8113..>8351
                     /locus_tag="OO568_003516"
     CDS             8113..>8351
                     /locus_tag="OO568_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6642712.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1917 a         2096 c         2673 g         1665 t
ORIGIN      
        1 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc
       61 atccggacac ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg
      121 aacgcgggga ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt
      181 cagttctcag gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc
      241 cccgactgcg ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac
      301 gggcaagttc tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa
      361 catcaatgcg ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt
      421 tttctgcacc atatatcagc tccatcaggg tgaccgaact gatacacatt ttcccctggt
      481 tcaggttaaa acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt
      541 tggtatcgag cataaacttc agcatcagaa tgactccctt tcctgcatgc cgggctgttc
      601 cctgttatcc ataaaatcgg tgctgacgct gtgtccgtcg aaccattcgt cccatgtctc
      661 tccggctggc gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt
      721 ttccggcaat gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac
      781 ggtggtttcc attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat
      841 tgataccggg atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg
      901 gccggaagtg ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg
      961 ggagaacgct gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag
     1021 gatgtcttta cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg
     1081 caggatggca gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt
     1141 atctccatga tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc
     1201 gtggattttg ctgtccgtca tcacccgtgt ggaggcactg gcctccacac gggtgatgac
     1261 ggacggacag tcagaaacgg tgctgaccgg taatctggtg atggcactgt ttaaccacga
     1321 caccagtcgc gatcaggaac cacagttaca cacgcatgcg gtggtgacta atgttacgca
     1381 gtataacggc gaatggaaga cactgagcag tgacaaagtg gggaaaacgg ggttcattga
     1441 gaatgtgtac gctaatcaga ttgcctttgg caggctctac cgggaaaaac tgaaagagca
     1501 ggttgaggcg ctgggctatg aaactgaagt ggtcggtaag cacggtatgt gggaaatgcc
     1561 gggtgtaccg gtggaggcct tttccggacg ctcacagact atccgggagg ccgtcgggga
     1621 agacgcctcg ctgaaatccc gggatgtggc ggcgctggat acgcgtaaat ccaaacagca
     1681 cgtcgatcct gaagtcagaa tggccgagtg gatgcagacg ctgaaggaaa ccgggttcga
     1741 catccgggca tatcgtgacg cagcggaaca acgggcatat acccgcacgc agacacccgg
     1801 acccgcttca caggacgggc cggatgtgca gcaggcggtg acacaggcca ttgccggatt
     1861 aagtgaacgc aaagtgcagt ttatgtatac ggacctgctg gccaggacgg tcggcatact
     1921 gccgccggaa aatggtgtga ttgaacgggc acgcgccggt atcgatgagg ccatcagccg
     1981 tgagcagctt atccccctcg atcgtgagaa ggggctgttc acgtccggga ttcatatgct
     2041 cgatgagctg tcagtccggg cactcagtcg tgacatcatg aaacagaacc gggtgaccgt
     2101 acatccggag aaaagtgtcc cccggacggc cggttacagc gatgccgtca gtgttctggc
     2161 acaggaccgt ccgtcgctgg ccattgtgtc cgggcagggc ggtgcagccg ggcagcgtga
     2221 gcgggtggct gaactggtca tgatggcccg ggagcagggg cgggaggtgc agattatcgc
     2281 tgctgaccgt cgctcgcaga tgaacctgaa gcaggatgaa cggctgtccg gtgaactgat
     2341 aaccggacgt cgtcagttgc tggaaggcat ggccttcacg ccgggcagta ccgttatcgt
     2401 cgaccagggc gaaaaactct ccctgaaaga gacgttaacc ctgctggatg gtgccgcacg
     2461 tcataacgta caggtcctga taaccgacag cgggcagcga accggtacag gcagtgcact
     2521 gatggccatg aaggatgccg gggtgaacac atatcgctgg caggggggag aacagcgacc
     2581 ggccaccatc atcagtgaac cggaccgtaa tgtccgctat gcccggctgg ccggagattt
     2641 tgcggccagc gtgaaagccg gagaagagag cgtggcacag gtcagcggag tacgggaaca
     2701 ggccatactg acacaggcca ttcgcagtga gctgaaaaca cagggcgtgc tcggacaccc
     2761 ggaggtaacc atgaccgccc tgtcaccggt ctggctggac agccggagcc gttatctgcg
     2821 ggatatgtac cgcccgggaa tggtgatgga gcagtggaac ccggagacac gcagtcatga
     2881 ccgctatgtg acagaacgtg tgacggcgca gagtcacagc ctgaccctgc ggaatgcgca
     2941 gggcgaaacg caagtggtgc gtatttcttc cctggacagc agctggtcgc tgttccggcc
     3001 ggaaaaaatg ccggtggcag acggtgagcg tctgagggtg acagggaaaa ttcccggact
     3061 ccgcgtctcc ggcggtgacc gcctgcaggt ggcatccgtc agtgaagatg cgatgacggt
     3121 tgttgtgccg ggacgggctg aaccggccac cctgcctgtg agcgattcac cgttcacggc
     3181 actgaagctg gagaacggct gggtggaaac gcccgggcat tccgtcagcg acagtgcgac
     3241 ggtttttgcc tccgtcacac agatggcaat ggacaatgcc accctgaatg gtctggcccg
     3301 cagtggtcgt gatgtccggc tgtattcctc actggatgaa acccgtactg cggaaaaact
     3361 tgcccgccat ccctccttta cggtggtttc tgagcagata aaggcgcgtg ccggtgagac
     3421 attgctggaa accgctatca gtctgcagaa agccgggctt cacacgccgg cacagcaggc
     3481 cattcatctg gcccttcctg tgctggaaag taaaaacctg gccttcagca tggtggacct
     3541 gctgacagag gcgaagtcgt ttgctgcaga aggaaccggt tttgctgacc tgggagggga
     3601 aatcaatgcg cagataaaac ggggtgattt actgtatgtg gatgtggcaa aaggctatgg
     3661 cacaggcctg ctggtttccc gtgcgtcgta tgaggcagaa aagagcattc ttcgccatat
     3721 tctcgaaggt aaggaggcgg tcacgccgct gatggagaga gtacctggcg aactcatgga
     3781 gaaactgaca tcaggacagc gcgccgccac ccgtatgata ctggaaacgt ctgaccgttt
     3841 cacggtggtg cagggctatg ccggtgtggg taagaccaca cagttccggg cggtgatgtc
     3901 agccgtgaac atgctgccgg agagtgaacg tccccgtgtc gtggggctgg ggcccacaca
     3961 ccgtgcggtc ggggagatgc gcagcgccgg cgtggatgca cagacactgg cgtcctttct
     4021 gcatgacacg cagcagcgca gcggagaaac gccggatttc agcaacacgc tgttccttct
     4081 cgatgagagc tctatggtgg gcaataccga catggcacgg gcatacgccc tgattgcggc
     4141 cggtggcggt cgggctgtgg ccagcggtga cacggaccag ctgcaggcca tcgcgcccgg
     4201 tcagcctttc cgtctccagc agacgcgcag tgctgccgat gtggtcatca tgaaggagat
     4261 tgtgcgtcag acgccggaac tgcgggaggc ggtatacagc ctgattaacc gggatgtgga
     4321 aagggcgctg tccgggcttg agcgtgtgaa accgtctcag gtgccacgtc tggagggcgc
     4381 atgggcaccg gagcactccg tgacggagtt cagtcacagc caggaagcga aactggcaga
     4441 agcgcagcag aaggcgatgc tgaaaggcga ggcttttccg gatgtcccca tgacactgta
     4501 tgaagccatt gtccgcgact ataccggcag gacaccggaa gcacgggagc agacgctgat
     4561 tgtcacgcac ctgaatgagg accggcgcgt actgaacagc atgattcatg atgcacggga
     4621 aaaggccgga gaactgggga aggtgcaggt catggtgcct gtcctgaaca cagcgaatat
     4681 acgcgacggg gagctgcgtc gtctctccac ctgggagaat aacccggatg cccttgccct
     4741 ggtggataat gtgtatcacc ggattgccgg tatcagcaag gatgacgggc tgataaccct
     4801 gcaggatgcg gaaggtaaca cgcggctgat ttcgccccgg gaggcggtgg ctgaaggcgt
     4861 cacgctgtac accccggaca ccatccgggt ggggaccggt gacaggatac gcttcacgaa
     4921 gagtgaccgg gagcgcggtt atgtggccaa cagcgtctgg acggtgacag cggtttccgg
     4981 tgacagtgtc acactttctg acggtcagca gacccgtgtg attcgccccg gtcaggagcg
     5041 ggcagagcaa catattgacc tggcctatgc catcaccgcc cacggtgcac agggggcgag
     5101 tgaaaccttt gccatcgcgc ttgaaggcac ggaaggtaac cggaaactga tggccggctt
     5161 tgagtcagcc tacgtggccc tgtcgcgtat gaagcagcat gtgcaggtgt acaccgataa
     5221 ccgtcagggc tggacggatg ccatcaacaa tgccgtacag aaaggaactg cccacgatgt
     5281 gtttgagccg aaaccggacc gggaggtcat gaatgcagag cggctgttca gtacggcgcg
     5341 ggagctgcgg gacgtggcgg caggccgtgc cgttctccgt caggcggggc tggccggggg
     5401 agacagtcct gcacggttta ttgctccggg acgtaaatat ccgcaaccgt atgtggcact
     5461 gccggcgttt gaccgtaacg gcaagtccgc cggtatctgg ctgaacccgc tgaccacgga
     5521 tgacggaaac gggctgcggg gattcagcgg tgaaggtcgg gtaaaaggca gcggggatgc
     5581 gcagttcgtg gccctgcagg gcagccgtaa cggagaaagc ctgctggctg ataatatgca
     5641 ggatggcgtc cggattgccc gtgataatcc tgacagtggt gtggtggtaa gaatcgccgg
     5701 tgaaggccgt ccgtggaatc ccggtgccat caccggtggc cgcgtgtggg gggatatccc
     5761 ggacaacagc gtccagccgg gagccggaaa tggcgaaccg gtcacggcag aggtactggc
     5821 acagcggcag gctgaagagg ccatccgccg tgaaacggaa cgtcgcgcag atgaaattgt
     5881 ccgtaaaatg gcagagaaca aacctgacct gccggacggc aaaacagagc aggctgtcag
     5941 ggaaattgcc ggtcaggagc gtgaccgggc tgccataact gaacgggaag ccgcgctgcc
     6001 ggagagtgta ctgcgtgaac cacaacgggt gcgggaagcg gtccgggaag ttgcccggga
     6061 aaatttgctg caggagcgtc tgcagcagat ggagcgggat atggttcgtg acctgcagaa
     6121 agagaaaacc ccgggcggag actgatacag gaagacaaac actgatgaca acggataaca
     6181 cgaacacgac acgtaacgat tcactggctg cacggaccga tacctggttg cagtcatttc
     6241 tggtctggtc acccggacag cgggatatca tcaaaacggt ggcactggtg ctgatggtac
     6301 tggaccacat caatctgata ttccagctga agcaggaatg gatgtttctg gccgggcgag
     6361 gagcctttcc gctttttgcc ctggtgtggg ggctgaatct gtcccgtcat gcgcatatcc
     6421 ggcaaccggc cattaaccgg ctgtggggat ggggaattat tgcccagttc gcatattatc
     6481 tggcaggctt tccttggtat gaggggaata tcctgtttgc ctttgcagtg gcggcacagg
     6541 tgctgacgtg gtgtgaaacg cgcagtgggt ggcgtacagc agcggccata ctcctgatgg
     6601 ccctgtgggg gcctttgtcc ggcaccagtt acggtattgc cgggttgctg atgctggcag
     6661 tcagcaaccg gctgtaccgg gcggaagaca gagcggaacg tctggcgctg gtcgcctgcc
     6721 tgctggctgt tattccggcg cttaaccttg ccaccagtga tgcagcggcg gtggccggtc
     6781 ttgtgatgac ggtgctgacc gttggtctgg tgttgtgtgc cggaaaatca ttaccccgtt
     6841 tctggcccgg agactttttc ccgacgttct atgcctgtca tctggctgtg ctgggcgttc
     6901 tggcgctgtg acgggtgtgg tatctttggc cgcaagagga tgattcgtca gaggcagaac
     6961 acagcatgat agagcagaag cgaccggtac tgacactgaa gcggaagaca gagggaacag
     7021 cgcctgtccg cagccggaaa accatcatca atgtcaccac gccaccaaag tggaaggtga
     7081 aaaagcagaa gctggccgag aaagcggccc gtgaagcaga gctggcggca aaaaaagcgc
     7141 aggccagaca ggcgctgtcc atttatctga acctgcccac gctggatgag gctgtgaata
     7201 ccctgaagcc ctggtggccg ggattatttg acggtgacac gccccggctt ctggcctgcg
     7261 gtatccggga cgtgttactg gaagacgtgg cgcagcggaa tatcccgctc tcgcataaaa
     7321 aactgcgcag ggcgctgaag gccatcaccc gttcagaaag ctatctgtgt gccatgaaag
     7381 ccggtgcctg ccggtatgac acggaagggt atgtgacgga gcatatttct caggaggagg
     7441 aagcgtatgc gggagcgcgt ctggcgaaaa ttcgtcgcca gaaccggata aaggcagaac
     7501 ttcaggccgt gcttgatgag aaataaaaaa agcctccccc agagagagga ggcagggaaa
     7561 taaggcttaa aaggaaataa cttccgcttc cgaaaggaag ccagtccact gaattattta
     7621 tcacaataat actgacaact aaagggggac aagaaaatga atggattcag aaacagttca
     7681 cggaacggcc aggtctggcg ttaccagcgt gccggcgggc gggccgttat tctggaagtc
     7741 agtggacgct ggatggaagc ggcagaagca tggcgacggg ctgcctgtgt tgcgcctagg
     7801 acagactggc agcagtttgc ccggaaaagg gctgaacact gccaccggcg ctgccgggga
     7861 agggtgtaac gaaaaaagca gccttcatta ctctaggagt cagaaggctg aaaggagttt
     7921 atgagatttg cttcttaata tccggtcggg cagtatgacc ggacagttct gagtagcaca
     7981 gagtcttttc cgcattaagt cgatttttta tattttgtga ttaatttcac aaaataaggt
     8041 gttattcaat gtgtgctgca ctattcaggg ggtctgaaat acaggtggca tttatctgat
     8101 atggagaata acatgaggaa atatattcca ctggtattat ttatcttttc atggccggta
     8161 ttgtctgcag atattcatgg acgggttgtt cgtgtactgg acggtgatac catcgaggtc
     8221 atggactcac tgaaggcggt gcggatcagg ctggtaaata ttgatgcacc ggaaaagaaa
     8281 caggattatg gccggtggtc aacagacatg atgaaatcac tggtcgcagg aaaaacggtt
     8341 accgtcacat a
//
LOCUS       DAJTYG010000133         8322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTYG010000133 DAJTYG010000000
VERSION     DAJTYG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.133"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            335..3184
                     /locus_tag="OO568_003517"
                     /pseudo
     CDS             335..3184
                     /locus_tag="OO568_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3284..5203)
                     /gene="dhaR"
                     /locus_tag="OO568_003518"
     CDS             complement(3284..5203)
                     /gene="dhaR"
                     /locus_tag="OO568_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR6642713.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATMTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5431..6501
                     /gene="dhaK"
                     /locus_tag="OO568_003519"
     CDS             5431..6501
                     /gene="dhaK"
                     /locus_tag="OO568_003519"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR6642714.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHHTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGR"
     gene            6512..7144
                     /gene="dhaL"
                     /locus_tag="OO568_003520"
     CDS             6512..7144
                     /gene="dhaL"
                     /locus_tag="OO568_003520"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR6642715.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7155..>8322
                     /gene="dhaM"
                     /locus_tag="OO568_003521"
     CDS             7155..>8322
                     /gene="dhaM"
                     /locus_tag="OO568_003521"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR6642716.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEE"
BASE COUNT         2174 a         2071 c         2074 g         2003 t
ORIGIN      
        1 tcccataact aattaggatt attacaaaaa atcttcacca aattttttaa ccgctgtttg
       61 ttgtaatatt tttgccatat tcatagtaac gaattggaat aagtaacgac aacttaggga
      121 ataactcgat ataaactcac ctttgtaagg ggggtgttaa atatatatta attcctgtgt
      181 tcctgatttt gcccggtaat agagaattat tctcagagaa gctcatcatg attgcaccat
      241 acagatatca tgatgataag aggatattat tcattgactt aaacaaaatg acaaaatcca
      301 tagacccata gcctgggtac tatataggag tggtatgggc atcaaacaac acaatgggaa
      361 taccaaagcc gatcgtctcg ctgaattaaa aatccgttcg ccctcaattc aactgataaa
      421 atttggcgct attggtttga atgcaattat cttttccccc ctgctgatag ctgctgatac
      481 aggaagtcaa tatggcacca atattactat taatgatggt gacagaatta caggagatac
      541 cgccgatcca tcaggaaacc tctatggtgt aatgacccca gcaggaaaca cgcctggcaa
      601 tatcaacctg ggtaatgatg tcaccgtcaa tgtcaacgac gcctctggat atgcaaaagg
      661 aatcattatt cagggcaaaa acagctccct gacagctaac cgactcacag tagatgttgt
      721 tggtcaaacc tctgccatcg gcattaactt aattggtgac tatacccatg ctgacttagg
      781 cacaggcagc accattaaga gtaacgatga cggcatcatt attgggcata gctcaacact
      841 aacagccact caattcacca ttgaaaactc gaacggtata ggcctaacca tcaatgacta
      901 tggcaccagt gtcgatcttg gaagcggaag taaaatcaag accgatggaa gtacaggtgt
      961 ttatatcggt ggtctcaacg gcaataacgc caatggtgct gcgcgtttta cggcgacaga
     1021 cctgacaatc gatgttcagg gctacagcgc catggggata aacgtacaga aaaactctgt
     1081 tgtcgatctc ggaacaaaca gtaccattaa aaccaatggc gataatgctc acggcctctg
     1141 gagctttggc caggttagcg cgaatgcact cactgttgat gtaactggag ccgcggccaa
     1201 tggcgtcgaa gttcgtggtg gtacaaccac tatcggtgca gatagccata tttcttccgc
     1261 gcagggcggt ggcctcgtca ccagtggttc agacgcgaca atcaattttt ctggcacggc
     1321 agcgcaacga aacagcatct tttccggcgg ttcttatggt gcctcggccc agacggcaac
     1381 ggctgttatc aacatgcaaa ataccgatat tacggttgat cgtaatggca gtctggcgct
     1441 gggtttgtgg gcgctcagcg gcggtagaat aaccggagac agtttggcta tcaccggcgc
     1501 ggcaggagcc agagggattt atgccatgac caacagccag atcgacctca cgagcgatct
     1561 ggtcattgat atgagtacac ccgaccagat ggccatcgca acgcaacatg acgatggtta
     1621 tgccaccagc cgcatcaacg cctcgggtcg tatgcttatc aacggtagcg ttctttccaa
     1681 aggtgggcta atcaatctgg atatgcaccc tgggtcggtt tggacaggtt cctccctcag
     1741 cgataatgtc aatggcggga aactggacgt tgcaatgaat aacagcgtct ggaacgtaac
     1801 aagtaattct aatctcgaca cgctggcgct gagccattca actgtcgatt ttgccagcca
     1861 cgggtcaact gccggcacat ttaccacatt aaacgtagag aacctgagcg gtaacagtac
     1921 ctttattatg cgtgctgatg ttgttggcga gggtaatggc gttaataata gaggggattt
     1981 attgaatatc agcgggagta gtgctggtaa tcacgtattg gctatccgca accagggcag
     2041 cgaggccaca acgggaaatg aagttctgac agtggtaaaa accactgacg gcgcggcctc
     2101 gttcagcgcg tcttctcagg ttgagttggg gggatatctg tacgatgtgc gtaaaaatgg
     2161 cactaactgg gagctttacg cttccgggac agttccggaa ccgactccta atcctgaacc
     2221 cacaccagct cccgctcagc ctcccatagt caaccccgat cctacgccta aacccaccac
     2281 gaccgcagat gctggcggca attatctcaa tgtcggttac ttattgaact atgttgaaaa
     2341 ccgtacgctg atgcaacgga tgggtgactt gcgaaatcag agtaaagacg gtaatatctg
     2401 gttgcgcagt tatgggggaa gcctggactc ctttgccagt ggcaaactga gcggctttga
     2461 catgggttac agcggtatcc agtttggtgg ggataaacgt ctctctgatg taatgccgtt
     2521 gtatgtcggt ctgtatattg actcaacaca tgcatcgccg gactatagcg gaggcgacgg
     2581 taccgcacgt tcagactaca tgggaatgta cgccagttac atggcacaaa acggttttta
     2641 cagcgatctc gttataaaag catcgcgcca gaaaaatagt ttccacgtac tggacagtca
     2701 gaacaactgc gttaacgcca acggcactgc gaatggaatg agcatctccc tggaagccgg
     2761 gcagaggttc aacctgtccc ctactggtta tgggttctat atagagccgc aaacccagct
     2821 tacatacagc caccagaatg agatggctat gaaggcgagt aatggcctca atatacatct
     2881 gaatcactac gaatcgctgc tggggcgtgc cagcatgata ctggggtatg acatcaccgc
     2941 aggcaacagc cagctgaatg tctatgtgaa gactggcgct atccgcgagt tttcagggga
     3001 taccgaatat ctgttgaacg actcccggga gaagtacagt ttcaaaggta atggctggaa
     3061 taacggcgtg ggagtcagtg cacagtataa caaacagcac acattctatc tctaagcgga
     3121 ttacacgcag ggtaacctct ttgatcagaa gcaagtcaac ggaggatatc gcttcagctt
     3181 ttaagtgcgg gatagacaag cccttaaacg atgctgaaaa ttgttagcgt gcgccggaat
     3241 gaaatacaga tcactctgat gtatcagaaa tcgtttctgt ctttcatacc cggcgcttaa
     3301 actgccctgc atcaatgcca tgttgcttca ttttccgcca cagcgtagtg cggccgatcc
     3361 caagtaaagc cgacatttcc tgaatgcgac cgcctgtgac ctgggctgcg ttaataattg
     3421 cctctttttc aacttccgca aatgacagac tggtggaaag gcgggtggcg ctgacatcat
     3481 ctgtcgcctg ctcggtaaac agatgttcag gtaaatcact gacgcgaatg cgcccgttat
     3541 cactactcag agcaagattc tcgatgacgc tgtaaagttc aaaatcgttg cctggccatg
     3601 cacaagaaac taggcgagcg agggcatcgt catcaatttt cagccgcgta gagaagcgtt
     3661 tttcaagact gcgtaatttg ttattcacca gcgccggaat gctgccacgc cgcatacgca
     3721 gaggcgggat ggtaatttca aatgcatgca gcgcgtaata cagctggcga ctaaaacgat
     3781 tttgttccac cagcattgcg aggtccgcgg tcattgttgc aatcactttg acatcaattg
     3841 gtattaaacg ccgcgcatcc agtcgcgtga taaccccctg cttgataacc tgcagcaaag
     3901 cagactgtaa ctccaccgcc agatattcaa tcttttcaag aaacagcgtg ccgccgtgtg
     3961 ccagttccag ccgactcaga cggccatttt cattgtccgt gcgatcgcca ccaataaatt
     4021 cttccgccag cgcagcatca ccatataact cacaattgac ggcgatataa ggacctgcag
     4081 cacgctcgct ttcattatga attgcctgac ttagcagtgc cttgcccacg ccctcttctc
     4141 cacaaagcag gacaggaaag ctactgcgcg ccgcctggcg accaaaatga atcaagcggc
     4201 gggtttgcgg atcgtcctgt ggcatatgag cgaaggtatg gctgactttt cctaattgac
     4261 tggtcattaa ctgccgcatc tgttccacag gatggagcaa caaaataaag ctggttccct
     4321 gcgtttctat tatcggttta agggttatca ccgcatcaat aaactggtgc tggctttcaa
     4381 aggttgcttc tacgtgtttg agcggatgtg cctgttttat tgcttgttgc aatacggcgg
     4441 gtaacgttaa gagttcagtg attgcccgtc cctgacttgc cgtcgcgtca aggcgcaaga
     4501 cccgcgccgc ctgggcatta ataaattgca aattaccctg ctcgtcccag ctaatcacgc
     4561 catcatccat actttctaac agggcattaa gttgatttaa atgacggtta gtttcagcga
     4621 gcaaactgtc cgtcagcagt aaattaccga cctcgcgggc gattgccaac gtcaacggca
     4681 aatcagctgc ggtagtttgc tcaaccggac acgccagcgc tattgttccc gtcaatcggc
     4741 ccttgctgtc aaacaacggc gttgcacaaa aggcccagtt ccagagtgcc tgtttgaaat
     4801 gttgatcggc catcgttttc acggcctgac cagagatagc cgctaacgat agcgcacaag
     4861 taccaataat tccctcggcg caatacgtgc cgtcattgaa tcccagtgca cttagctgct
     4921 gcaaggtttg cggatcgcca ttacggctga gaatgcaggc ggtttcatcg aggataaaca
     4981 gcgcacactc tcgcggtgcc atatattccc aggcatcttc cagcgcagcc tgcccgagcg
     5041 tcagcatcgc tttcttacgc cgataaatag aagcaaatgt cacgccctgg gcctgatgtg
     5101 gtacgttcca tgtctcccgt ttcatcagct tattgcatcg ctcccaggag gttgcaatta
     5161 agggagatat gccccgacca tcgttgttaa aagcgccact catatcccgc atcccttatg
     5221 acaaaaggtg ttccggctcg caattttaag tattaataca cgtaaaagtt tcaatatgga
     5281 acattcatta aagcatatgt ttcactatga aacataattt gggctaattt tcttttccct
     5341 tgcctgatgc acaatggatt catcggcagt cagtggtcgc cgtgtcgttg aacatcatcc
     5401 atgccctacc gtaattgctg gcgcaaaata atgaaaaaat tgatcaatga tgtgcaagac
     5461 gtactggacg aacaactggc aggactggcg aaagcgcatc catcgctgac actgcatcag
     5521 gatccggtgt atgtcacccg agctgatgcc cctgttgcag gaaaagtcgc cctgctgtcg
     5581 ggtggcggca gcggacacga gccgatgcac tgtggttata tcggtcaggg gatgctttcg
     5641 ggggcctgtc cgggcgaaat tttcacctca ccgacgcccg ataaaatctt tgaatgctcc
     5701 atgcaaattg atggcggcga aggtgtactg ttgattatca aaaattacac cggcgatatt
     5761 cttaactttg aaacagcgac cgagttactg cacgatagcg gcgtaaaagt gaccactgtg
     5821 gtcattgatg acgacgttgc ggtaaaagac agtctttata ccgccgggcg acgcggcgtt
     5881 gccaacaccg tattaattga aaaactcgta ggcgcagcgg cggagcgtgg cgactcactg
     5941 gacgcctgtg cggaactggg gcgtaagttg aataatcaag gccactcaat aggtatcgct
     6001 ctcggtgcct gtaccgttcc tgccgcgggc aaaccttctt ttacccttgc ggataatgag
     6061 atggagtttg gcgtcggcat tcatggtgag ccaggtattg accgccgccc tttctcttcc
     6121 cttgatcaaa ccgtcgatga aatgttcgac accctgctgg aacatggctc ataccatcac
     6181 actttgcgtt tctgggatta tcaacaaggc agctggcagg aagaaccaca aaccaaacaa
     6241 ccgctccagt ctggcgatcg ggtgattgca ctggttaaca atcttggcgc aactccgctt
     6301 tctgagctgt acggcgtcta taaccgcctg accacacgtt gccagcaagc gggattgact
     6361 atcgaacgta atttaattgg cgcgtactgc acctcactgg atatgaccgg tttctcaatc
     6421 accttactga aagttgatga cgaaacgctg gctctctggg acgccccggt ccacaccccg
     6481 gcccttaact ggggtagata aggagaaagc aatgtcactg agcagaactc aaattgttaa
     6541 ctggctcact cgttgtggcg atattttcaa caccgagagc gagtatctta ccgggctgga
     6601 tcgcgagatt ggcgacgctg accacgggct aaatatgaac cgaggcttta gcaaagtggt
     6661 ggaaaaactc cctgctatcg cagataaaga tatcggtttc attctcaaaa ataccggtat
     6721 gacgctgctt tccagcgtcg gtggtgccag tggtccgctg ttcggtacct tctttatccg
     6781 cgccgcacag gcgacccagg cacggcaaag cctgacactg gaagagcttt atcagatgtt
     6841 ccgcgatggc gcggacggcg taatcagtcg cgggaaagcc gaacctggcg ataaaaccat
     6901 gtgtgatgtg tgggtgccgg tggtggaatc gttacgtcag tccagcgagc aaaatctctc
     6961 tgttccggtg gcgctcgaag ctgccagtag catcgccgaa tccgcggcac aaagtacgat
     7021 tacgatgcaa gcccgcaaag gccgcgccag ttatctcggt gaacgcagta ttggtcacca
     7081 ggatcccggc gcgacctcgg tgatgtttat gatgcaaatg ttggcgttag ccgcaaaaga
     7141 gtaaggaatt ggtgatggta aacctggtca tagtttcaca tagcagccga ctgggagaag
     7201 gtgtcggtga attagcccgt cagatgttaa tgagtgatag ttgtaaaatc gccattgccg
     7261 cgggaattga cgatccacaa aatcccattg gtaccgatgc cgtcaaagtg atggaggcca
     7321 tcgaatctgt tgctgatgcc gaccatgtgc tggtcatgat ggatatgggt agcgcattat
     7381 tgagtgctga aactgcgctg gaattgctgg ctcccgagat cgccgcaaaa gtacgtttgt
     7441 gtgctgcgcc gttggtcgaa ggtacactgg cagcaacggt cagcgcggcc tcgggggcgg
     7501 atatcgacaa agttatcttt gacgctatgc atgcgctgga agccaaacgt gaacaactgg
     7561 gtttaccgtc ctccgacact gaaatctctg acacatgtcc tccgtacgat gaagaagccc
     7621 gttctctggc ggtggtcata aaaaaccgta acggcctgca tgtacgtccg gcctcccggc
     7681 tggtttatac cttatcgaca tttaatgccg atatgttgct ggaaaaaaac ggcaaatgcg
     7741 tcacaccaga gagtattaac cagattgcgt tactacaagt tcgctataac gatacgctgc
     7801 gcctgattgc gaaagggcca gaagctgaag aggcactgat cgctttccgt cagctggctg
     7861 aagataactt tggtgaaacg gaggaagtcg ctccacctac tctgcgtccc gttctgcctg
     7921 tttcgggtaa agccttttat tatcaaccag ttttatgtac ggtacaggca aaatcaaccc
     7981 tgaccgcgga agaagaacaa gatcgattac gccaggctat tgacttcacg ttattagatc
     8041 tgatgacgtt aacagcgaaa gcagaagcca gcgggcttga cgatattgcc gcaatctttt
     8101 ctggtcacca tacactgtta ggtgatccgg aactgctggc ggcggcaagc gaactccttc
     8161 agcatgaaca ttgcacggca gaatatgcct ggcagcaagt tcttaaagaa cttagccagc
     8221 aataccagca actggatgat gaatatctac aagctcgcta tattgatgtg gacgatcttc
     8281 tgcatcgcac cctggtccac ctgacccaaa cgaaagaaga ac
//
LOCUS       DAJTYG010000134         8271 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTYG010000134 DAJTYG010000000
VERSION     DAJTYG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.134"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..484)
                     /locus_tag="OO568_003522"
     CDS             complement(<1..484)
                     /locus_tag="OO568_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6642717.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     Y"
     gene            complement(571..932)
                     /locus_tag="OO568_003523"
                     /pseudo
     CDS             complement(571..932)
                     /locus_tag="OO568_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1272..1826)
                     /locus_tag="OO568_003524"
                     /pseudo
     CDS             complement(1272..1826)
                     /locus_tag="OO568_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1969..2091
                     /locus_tag="OO568_003525"
                     /pseudo
     CDS             1969..2091
                     /locus_tag="OO568_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2353..2919)
                     /gene="ipgB2"
                     /locus_tag="OO568_003526"
     CDS             complement(2353..2919)
                     /gene="ipgB2"
                     /locus_tag="OO568_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR6642718.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(3201..3515)
                     /locus_tag="OO568_003527"
     CDS             complement(3201..3515)
                     /locus_tag="OO568_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642719.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3593..3703)
                     /locus_tag="OO568_003528"
                     /pseudo
     CDS             complement(3593..3703)
                     /locus_tag="OO568_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4027..4704)
                     /gene="ospD1"
                     /locus_tag="OO568_003529"
     CDS             complement(4027..4704)
                     /gene="ospD1"
                     /locus_tag="OO568_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR6642720.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNSHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(5075..5212)
                     /locus_tag="OO568_003530"
                     /pseudo
     CDS             complement(5075..5212)
                     /locus_tag="OO568_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5294..5592)
                     /locus_tag="OO568_003531"
                     /pseudo
     CDS             complement(5294..5592)
                     /locus_tag="OO568_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6181..6340)
                     /locus_tag="OO568_003532"
                     /pseudo
     CDS             complement(6181..6340)
                     /locus_tag="OO568_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6344..7573)
                     /locus_tag="OO568_003533"
                     /pseudo
     CDS             complement(6344..7573)
                     /locus_tag="OO568_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7593..7940)
                     /gene="tnpB"
                     /locus_tag="OO568_003534"
     CDS             complement(7593..7940)
                     /gene="tnpB"
                     /locus_tag="OO568_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6642721.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7940..>8271)
                     /locus_tag="OO568_003535"
     CDS             complement(7940..>8271)
                     /locus_tag="OO568_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6642722.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQILIR
                     EIKGSSH"
BASE COUNT         2120 a         1940 c         1824 g         2387 t
ORIGIN      
        1 cataacgcat attgtcagtg aattcgatgt acaacattcg gctgtatccg ggaacagcaa
       61 cgaacacgtg aagcggtgag cgaccattac gcatagtgcc ccagtcaacc tgcatctgtc
      121 gtccgggttc agtttcgaac cgaacggcag gctcctgctc ctgaggaacc gagagagaac
      181 gaatgaatgc cctgagaatg gtcattccgc cacgatatcc ctggtctctg atctcgcgag
      241 cgattaccgt tgccgggatt ttgtaaggat gagcatcggc gatgcgttga cgaatataat
      301 cccggtattc atccaggagt gaagcaacag caggtcgcgg cgtatatttt ggcggctcag
      361 attttgcctg caaataacgt ttaacggtat tgcgggagag ccccagttct ctggcaatcg
      421 cccggctact cattccctgc ttgtgcagga ttttaatttc cataactgtc tcaaaagtga
      481 ccataagctc tcctgaatca ggagagcaga ttaccccctg gatctgattt caggcgttgg
      541 gtgtggatca ctattgcacc gttcatgaca gtgttgttgc gccttgctgc caacgaagct
      601 ccactgccca tcaagttcac agataagggc aacatcagcg tgagcgactg gcgcagatgt
      661 tatccgccgg ggcgggagct ttttaatgtc cgtatgacgg tattgatgcc gattttcagc
      721 gtacgggcag tatcgcgaac tccggcaccg ttgaaagcca tttcagtgat ttggttttta
      781 actcccggtt tacgggcctc gtaacagtaa gtgagctgaa acacccggtg acagtcacgg
      841 caacggaatc gatcatgtcc ttttgggttc tgcccgtgac ggtaaacctg aacggactgg
      901 caacgggggc aatgaaagtt aactgtgacc atgaaagaac cttaaaagcg ggtattataa
      961 cccctaattc aactaattgg aggcattgtc atttttaatc tatggataca cttaaaaatc
     1021 ctctcacctt acgttaaaaa ccttctcatg tttagagttt tttattatgt ttatcataat
     1081 gatatgacga tctgagtttt taaaatcaag aagaaaagat aagtttttta aatgcacagt
     1141 gcctgtggat aactttgcgt aaatccggag gacagatcac tctgaagcaa gacaacagca
     1201 tctgaaaaca atatgctcac gtttcaacca cagggcgtaa gtgatcctac ccacgtaata
     1261 tggacacagg cctaagcgag gttctggttt tcaaattgtt ccggactgag gccgccacac
     1321 caactgtgcc gtcgccagcg attgtaatca cattcgatat aattaaacac tgttgcccgc
     1381 attatttccc ggctgataaa gtgttctccg tggagacatt ccactttcag cgaatgaaag
     1441 aagctttcca cgcaggcatt atcgtagcag cagccctttg cgctcatact gccacgcaga
     1501 ttatgccact tcagcagcgc ctgataatct gctgaacagt actgacttcc tcggtccgag
     1561 tgaacgataa cgttccgggg tctcttacgc cgccacagcg ccatctgcag ggcatcgcag
     1621 gccagttgcg ccgtcaggcg tggtgacatg gaccagccaa taacggcacg tgaccacagg
     1681 tcaatgacca cagccagata cagccagcct tcatctgtac gtaagtacgt gatgtctccc
     1741 gcccacttct ggttcggacc actggcgtaa aaatcctgct ccagcagatt ttctgacaca
     1801 ggcaggccat gtgcgcggta actgaccgta ttgctgggta agatcaattt gctcactggt
     1861 atttactacg tgcatcgaat gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg
     1921 gttccgtaag cagccggaga gtgaaactct ctccggctga ctcgactcgc aggttccgac
     1981 agcggtgacg aacatgcagc ggtgttgttc tcgctgatcg gcacatgccg tctgaacaat
     2041 gtggagccag aaaaatggct gcgttacgtt attgaacata tccaggactg accggcaaac
     2101 cgggtacgcg atctgttgcc ttggaaagtt gatctgactt ctcagtaaat atcaatatga
     2161 ttctgacgag ccgcttacct ttgagcagag aaaggggaga gggatcccac atccatgagc
     2221 taggaaaata caaaaactgg ctatacaaca attcaatata ttaggactta cttgcgagga
     2281 gaaatatagc accgctcagg caattcctgg gtatttactt ttcttagttt ccttactcgg
     2341 gatgttcttt catcagaaag gcgattctaa atttgtaata tagtcactca tattcccacg
     2401 taatttcttt agatcaatat attgcacatt tttctcaaaa aactgatcgg aaataagatt
     2461 tgcaacattg cttgttgtat catttttaac gcttctattc atcccttcac aaagttcgtc
     2521 cattttcttt ttgaaataac tgcttcctga tacaagatga tttatggaac tttgtgcagc
     2581 attgatgtct agagaaatgc gataatcttt ctcaacctgt ttaaatatct gtttacggag
     2641 attatcgccg atttttcggc tgtgttctgc acaaaactta tcaatacatt ggttcaccac
     2701 ccgggatacg tagacttttt tttcatcatt tttccaatct gtaatatttt caccgatttg
     2761 ttctgaaaca gagctcaaaa tactagtatt tattttggtt gatgatattt ttactatcct
     2821 tttccccatt gtacagcgga taatctcatc cacctttcct gaaaaatatc ttttgtgaga
     2881 taaactgatt ccaaaattat taaaagatgt tccaagcatt ataatcactt tgtcatgtaa
     2941 aatagtaaca ttgtcattat tttagctgat tcaaacaatt ctgtaaatgc tttgagttct
     3001 ttactccctt ggctcaatga tggaccgtct ggaatcccag agactaaact ctctgagaaa
     3061 gagagtatga aaaaacttgg ggcagtccct aaatgaaggg accaacagcg gtaaaataac
     3121 cggcatcatt cataatgatg ccctgttagt tgtagttatg aagatttacg tcattaaaat
     3181 tgctgttcgt agtgtcagtc ctatggtctg gcatcagctg aggatcgtag ccgatacatc
     3241 actggcttca cgacattcgt gttgaggcta ttcatgaaaa ctccacgctg aaaacgccgt
     3301 tttgtataaa cggtcacggt atgcccggag ccactgttgc tgatgaattc gataaaacct
     3361 tggcgtttct tgaagctatc gttaatgcgg ataatgaaac aacgattggt gagattcgct
     3421 cttttgccga cgccctggac gctgtccggt tcaatcgcaa caaaatcaac cggcagctga
     3481 gcaaacccaa ccttgcctct ctgccgctgg agcatgaagt gatttggctg ggacgtagcc
     3541 gctgacagct tctataaaag tatgatccta cccacgtaat atgaacacag gcttaagtga
     3601 ggttctggtt ttcaaattgt tcaggactga gacctccaca ccaactgtgc cgtcgtcagc
     3661 gattgtaatc gcacttgata taattaaaca ctgtatattt catttgtgga gtctccagtc
     3721 tgagggggct ttatgcattc cgtattgggc actatgatgc cagaaatctg caaggctcca
     3781 caccttcctg tatttccctt cctctatcac agtgcacttt acttaatagg gtgggatgtg
     3841 ttcattacat ttattgtgtt ttttatgatt gtttttgcgt ccagtgtcgt tcgtcatggg
     3901 aaactggttc tatgggtagt atcgctcagt ctagttaact gtttgtttct ttttagaaat
     3961 taagcagatc acacaattcg gggagagatc cttctaagtg caattctttg cagctattta
     4021 gcacacttaa aacaactctt gtattttgtc agaaataata ttaaacatgt cttgttcatt
     4081 attgttaagt ctgtctgcta tttctgtaag ttttatccca ctatgatggc atatatttat
     4141 atatgccatc atagcctcga tattcccttt ccctgcggcc agaaataatc ctggtaaccc
     4201 ctcattatct cttacatcca atagtttaat cgtttctttt ggtgataaat caaaagtttt
     4261 aataaaatct ccataagctt gaatcgcagc ggaatgacct tcttgaagag ctatgaacaa
     4321 agcagattcc ccagcattat ttcgtgctgc aagaacttct agtttttcct ctttgggtat
     4381 ttcggctgcc aacagcgatt caccgtattt acgcgctgca tcggcatttc catgccaaat
     4441 caatgaaaac aaattatggc tgttatactc cttattattc tttgtcggaa tggatatgct
     4501 ctgtattttg gccaattttc tgaaagaaac accactatat cccccccctg atttttcttc
     4561 attgatggcg tgggatatag cggtatttgt cacgttaatg atattatttt tcattccttt
     4621 attttcatta gcagtattgc tgcctattat taggtgcata tttttgttgt ttgctggatg
     4681 taatccatag ttatttattg acatttttgt tcatcctttt tgctaaaaaa aacaataacc
     4741 tatttttgta atgtaataga tattagtcta ttgctaatct aaaaaaacca ttgccacatt
     4801 gaatggctat attcaccaat aaattctttc gatttcgtat attgacatat cgtagatata
     4861 tcttatccct aagattcaat aatcgagtcc gtttaccatt ctgtgcaagc gccttcactc
     4921 agaagtgacc gtcaaggcga tcaccaaact tgataataaa gcagctcatt gccatacgcc
     4981 agtccttaag gggcgttgtc catttctgtg atgctgccta gatttaactg ccagctccgg
     5041 cttatctaca tctaaatatg tgagcctaat accgctacac tttgtccaga ctatgttttt
     5101 ctccgggaag tgggctaacc gtctccataa agtgacgaac ttttttcaac agtggccata
     5161 ttgaacggca ctgatgatta cgggttattg tctcatgaag agccggccac atcactgaca
     5221 gcagtgactt caggccttca gcaccagaca gcctgaacca gttaatccat cgcccgacag
     5281 atttagtaat ctagggctgg tttttcttca gctgttgcgg ttcgaaagtg ccattacgat
     5341 cacgcagagt acggatttcc agaggttcat ctccagtagt gataatcttc gttaagcaac
     5401 cattgcggga attgctgcca gatctaagct gatttttctc gcacccaaga tgatgagtca
     5461 tattagcatt gagtacggtt tcattttcag catccaatca aactgactga gatcttcagg
     5521 atttttgaga cttttggcta gttcattagc taaagcccgc aacttattag atgtcaactg
     5581 tttttcattc ataaattcac cttcatttga tatcaaatta aatagttcaa catcagagaa
     5641 ttatacaaac ttatgtacag gctcctatga tcttcgaatc ctgaagatta tagtgaaaat
     5701 aagttttcac gttgaaatcc cacatagtta gtatatgata aataatcatg gatgacctga
     5761 ttaacctctt ggggtagtgc ttttatagaa gaataaatat tctactaatt taatacatca
     5821 cctaaaatag tattgttttt tatggttggt tgaaggtcgt ggtgatgatg ttagtccttt
     5881 tgagaatatt ccagtgccag atcttactta taataaaggg gcttggcagt ttggataaac
     5941 tattgttctt attccactaa tacaaacttt ttgtaaagta tattaaatat acttcatttc
     6001 atactgaaat ccccggaagt attcaatcaa acaattacac cataaaagga agagggggcc
     6061 ctaattaagt tgtcaagcat gtttatgata cctgcagggt ataaattttc tggctccaag
     6121 cagacacata taaaacgtct ttattgatcc tacccacata atatggacac agtcgtaagt
     6181 gaggctctgg ttttcaaatt gttccggact gagaccacca caccaacggt gccgtcgcca
     6241 gcgattgtaa tcgcactcga tataactaaa cactgttgcc cgcactattt cccgatggat
     6301 aatagtgttc tccgtggaga cattccattt tcagcgaatg aattttcatc tgttgctgta
     6361 tccagtcata cagtgactgc atcagtggcg cggctctggc ttttcttgcc gccagacgct
     6421 gttctgctga acatccccgg acttctgcct cgatggcata cagttcaccg atacgctgca
     6481 gggcttccgt ggtgatgtcg gttggcactc ttgcatgcac atcgtggatt tttctccgga
     6541 catgagccat acatgcggct tccgttattc tgccggattc gtacaacgcc cggtaaccac
     6601 cgtaagcatc ggcctgaagc actccgctgt aaccggccag atgattctgt ggatggatac
     6661 ctttccggtc cggtgagtac gcgaaccaga ccgccggagg catttccgaa ccggcgttac
     6721 ggtcatcacg aacgtagacc cacagccggg cagtccttgt tttaccgctg cccggctccc
     6781 ggaccggaac ggggatatca tcggcatgga ctttaccggg catcagcaca tactggcgca
     6841 ggatgtcata cagcggttcc agcagttcag caacagcacc tgtccagcgc cccagtgtgg
     6901 cacggctcag ctccacgccc tgacgacggt atatttctga ctggcggtat aacggcagat
     6961 agtctgcata ttttccagcg acaacatggg ccagaagccc cgctccggca taactgcgtg
     7021 caatgggttt tgaaggcacc ggtgcctgca cgatatggtc acaccggcaa caggccagtt
     7081 tcggacgttg tgtttcgata accttaaagg cgctgctgat aagctccagc tgctctgaca
     7141 catcacatcc cagagaactg agttcgccac cacaggccgg gcagcattcc tcttccggac
     7201 ggatgacccg ggtttcacgg ggaagtgagg caggtaacgg tttacgggct gaagactggc
     7261 gcaggggaga tggcagtacc gggtcatatt gctcacccag cgtttccgcc atttcttcct
     7321 gaagtgcgct gattcgctcc tgtgcttcca gtatctgccg ttcggttttt gcgcgaagtt
     7381 tttctgagct tttaccgaac tgcatgcgct gcagtttcgc aaccagcgcc ttcagccggt
     7441 tgatttcgga agcataagcc gccacccgct gtgagagcag gcggttgtat ttcgccatct
     7501 ggcggatggt gtcctgttgc gtctgcaaca gtgcccgcag gcgggcattc tcatgagaaa
     7561 gtgaggtgtc catatcctca ctttacaacg ggttatatgc ggatgccagc gcgttccgtt
     7621 cgtttcgggt gtttccagtc gatgccctca agaagcatgg ataactgggc cggagtaagg
     7681 tgcaccttgc cgtcacgggt gaccggccag acgaagcggc cccgctccag gcgtctggta
     7741 aagaggcaca gtccgtcact gtcagcccac aacactttta tctggtcacc ccggcgtccg
     7801 cggaagatga acagatgccc ggagaacggg tcatccttca ggacgttctg aacttttgag
     7861 gccaggccgt taaagccatt tcgcatatcg gtgatacctg caaccagcca gatacgcgaa
     7921 cctgcaggga aagatatcat cagtggctgc tcccttttat ttcgcggata agtatctgta
     7981 ataacgccgg cgtcagttta cctttaagcc tgagagttcc ggccggcaga accagctcac
     8041 agcacagact gtcggacggt gtatttatct gctccggcgt taacgtcacg ggaagcagtg
     8101 caggcatatt ttttccggaa ggcagcaggc cacctttccg gtattgatgg cgccagttga
     8161 agagcaggtt atcgttgatt ccgttttcac gggcgatctg cgccacacag gctccgggct
     8221 gcagtgactg ctccactaaa gcgattttaa attcataagg aaaattaggc c
//
LOCUS       DAJTYG010000135         8200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTYG010000135 DAJTYG010000000
VERSION     DAJTYG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.135"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            59..460
                     /locus_tag="OO568_003536"
     CDS             59..460
                     /locus_tag="OO568_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6642723.1"
                     /translation="MSSNFCERLEQWGNKSVNNGEERAIAVERIKEAYNSNMASLDLS
                     YLDLSELPPIPSTVNTLNLENNCLTCLDLADIASLVNINLSVNKINTITFPKEPKLEN
                     IYIDHNNLESLDLKNQHSLVNLEAQNNSLKK"
     gene            570..1901
                     /locus_tag="OO568_003537"
     CDS             570..1901
                     /locus_tag="OO568_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6642724.1"
                     /translation="MITDLILHNHPKMKTITLNDNHIAHLNAKNTTELEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYTPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            2225..3613
                     /gene="narU"
                     /locus_tag="OO568_003538"
                     /pseudo
     CDS             2225..3613
                     /gene="narU"
                     /locus_tag="OO568_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
     gene            3695..7328
                     /gene="narZ"
                     /locus_tag="OO568_003539"
                     /pseudo
     CDS             3695..7328
                     /gene="narZ"
                     /locus_tag="OO568_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            7325..>8200
                     /gene="narH"
                     /locus_tag="OO568_003540"
     CDS             7325..>8200
                     /gene="narH"
                     /locus_tag="OO568_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6642725.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKYIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTEREVDL"
BASE COUNT         2192 a         2030 c         1910 g         2068 t
ORIGIN      
        1 ttgtgtaaaa aataatgcca gttatcaact taataacgca ttacctaaca aagaaaccat
       61 ctccagcaat ttttgtgaac gactagaaca atggggtaat aaatcggtta ataatggtga
      121 agaaagagca attgccgtgg agcgaattaa agaagcttac aattcgaaca tggcatctct
      181 tgatttgtca tatcttgatt tgagtgaatt accgcctatc ccctcgacag ttaatacgtt
      241 aaatctggaa aacaattgtc taacttgtct tgacttagct gacattgcca gtctcgttaa
      301 tatcaacctc agcgttaata aaattaacac gataaccttt ccaaaagaac caaaactgga
      361 aaacatttat attgatcata acaatctgga aagtttagat ttaaaaaatc agcattcatt
      421 ggttaatctg gaagcgcaaa ataacagcct aaaaaaatga tatttctgat agttataaac
      481 tgaaatttct taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat
      541 ctctgattga gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc
      601 ccaagatgaa aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca
      661 ctacagaact ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg
      721 atcaactaat atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc
      781 cacttgcaca aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag
      841 tgaccattga tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca
      901 cctctatcaa tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac
      961 tcgtaaagct aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa
     1021 atgtaagtat tacacatata tctatgaata ataatgctct cagaaatatt gatatagata
     1081 ggctttcatc agttacttat tttagtgcgg cacataatca actagagttt gtgcaattag
     1141 aatcttgcga atggctgcaa tacctgaatc tcagccataa tcaattaact gatattgttg
     1201 caggtaataa aaatgaactc ttactgctgg atctatccca taataaacta acaagtttac
     1261 acaatgtctt atttcccaac ttgaatacgt tacttattaa caacaatttg ctttctgaaa
     1321 ttaaaatatt ctatagcaac ttccgcaatg ttcagacatt aaacgctgct aacaaccagt
     1381 tgaaatatat aaatcttgat ttcctgactt atcttccatc tatcaaaagt ttaagactgg
     1441 acaataataa aataacccac attgatacta ataatacatc cgatattgga actttattcc
     1501 ccataataaa acagagcaaa aacttaaatt ttttaaatgt ttctgggaag aacaattgcc
     1561 ctactatgca gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc
     1621 tggcaattct ttcgcctggt gcatttgaag ttcactctga cggaatagat gtggataacg
     1681 aattgtttca ctatactatt aaaaaagcat ataccccata taatatacac acttacaaga
     1741 cagaggaagt tgtaaaccag aggaatataa aagttaaaaa catgacctta gatgaaataa
     1801 acaatactta ctataataac gattattaca atcaggcaat aagagaggaa ccgatagact
     1861 ttctggacag atcgttttcc tccaattcat ggccttttta gtgaataaat catcactaat
     1921 tctataagtg tctggtcttg ttgagaatat agttaatatg taacccttta gattcacgat
     1981 ctgaagggtt gaaacatgac atacgttcaa agattaaccc ccgtttgaag gttagcttgt
     2041 tagttacatt tagtaacaca tatctggcta cttaatcccc attctgcccc cgcggttacg
     2101 ctttcttatc tattctttca atcagcactt cacccaattc atccttaatt ctaataactg
     2161 gctattgcgc cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa
     2221 caatatggca ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga
     2281 aaatccggcc ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc
     2341 agtcagttgt ctacttcttg ccttctgtat ctagatgcta tttagcgcag ttaccgttaa
     2401 tctcaataaa atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc
     2461 ctccgtttct ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg
     2521 acgccaatgg ccggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat
     2581 tgccgtgcaa aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg
     2641 ttttgcaggt gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc
     2701 caaacaaggg agcgctcttg ggattaatgg cggattagga aacttaggtg taagtgtaat
     2761 gcagctggtt gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg
     2821 cgtaccgcag gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc
     2881 gctactggcg attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg
     2941 cgcctcaatt gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct
     3001 gctttacctt gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc
     3061 aaaaacccag ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttattgg
     3121 tgccatcgcg cggtcggttg gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac
     3181 gttgatcaac tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg
     3241 cacaggctcc ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgaccgc
     3301 gggtctggga agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat
     3361 ttatcgggtg aagatgaaag gcggtagtga tgagcaagct cagagagaag ccgtcaccga
     3421 aacggcggca gcgctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc
     3481 gcaggcattt ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt
     3541 tttaatcttc tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt
     3601 cagccaaaaa taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca
     3661 gatcccaatt cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg
     3721 ctacttcaaa caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa
     3781 ccgcgactgg gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac
     3841 ccacggcgtt aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt
     3901 gacctgggaa atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga
     3961 acctcgcggc tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct
     4021 gaaatacccg ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaaacaaca
     4081 cagcgatccg gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta
     4141 caaacaagtg cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct
     4201 gattgccgcc gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt
     4261 ctcgccgatc ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcgct
     4321 gcttggcggc acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc
     4381 gatgacctgg ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta
     4441 catcatcgct tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac
     4501 cgaagtacgc tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc
     4561 caaattgtgc gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc
     4621 aatggggcat gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa
     4681 ctactgccgc cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg
     4741 tagctacgtt cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag
     4801 caacaatccg cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa
     4861 cggttcgatt ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc
     4921 cggtacggaa accgaattgt cgttaaccct gctcggtcaa catgacgctg ttgcaggcgt
     4981 ggccttcccc tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc
     5041 ggtgctggtg cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc
     5101 agtggtcagc gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga
     5161 tgaaaacagc gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca
     5221 aattaccggc gtaccgcgcc agtatattga aaccatcgcc cgcgaatttg ccgataccgc
     5281 ccataaaacg catgggcgct caatgattat cctcggcgca ggtgttaacc actggtatca
     5341 catggacatg aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca
     5401 aagcggtggc ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg
     5461 gttgccactg gcctttgcgc tcgactggaa ccgaccaccg cgccagatga acagcacctc
     5521 gtttttctac aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact
     5581 ttcaccgctc gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc
     5641 cgaacgtatg ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa
     5701 agctgaagcc gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc
     5761 gggcgattta cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt
     5821 gtttgtctgg cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa
     5881 gtatctgctg gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat
     5941 caaaccggaa gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt
     6001 gacgctcgac ttccgcatgt ccagtacctg cctgttctcc gatatcgtcc tgcccaccgc
     6061 cacctggtat gaaaaagacg atatgaacac ctcggatatg catccattta ttcatccgct
     6121 ttctgcggcg gtcgatccgg cctgggaatc acgcagcgac tgggaaatct acaaaggtat
     6181 tgccaaagca ttttcgcaag tgtgcgtggg ccatcttggc aaagaaaccg acgtggtatt
     6241 acaaccactg ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg
     6301 gcgcaaaggc gaatgcgatc tgatcccggg taaaaccgcg ccgaatattg tggcggtgga
     6361 gcgcgactac cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact
     6421 tggcaatggc ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa
     6481 actcaattac accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat
     6541 tgacgcttca gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa
     6601 agcgtggcag gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa
     6661 agaggacgag aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag
     6721 ccccacatgg tccggtctgg aaagcgatca cgtatcctat aacgcgggat acaccaacgt
     6781 tcatgagtta attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc
     6841 gtggatgcgt gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag
     6901 cgtcagtgag atgcgccaga taccgccaaa cggcttccag gaaaaagcac ttaacttcct
     6961 gacgccgcac cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac
     7021 gctctctcgc ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc
     7081 aggaacgcat tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata
     7141 acttggtcac ccgcgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg
     7201 cctggggctt taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc
     7261 gcaagatgaa gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga
     7321 aaaaatgaaa atacgttcgc aagtcggcat ggtgcttaac ctcgataaat gtatcggctg
     7381 ccatacctgt tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggca tggagtacgc
     7441 atggtttaac aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca
     7501 ggaagagtgg caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg
     7561 cagcaagatg ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agcagattga
     7621 tgattactac gaacctttca ccttcgacta cgaacattta catagcacac cggaaggcaa
     7681 acatatccct actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat
     7741 ctggggccca aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg
     7801 caacttcgag gccatgcaaa aggagatgta cgggcagttt gaaaacacct tcatgatgta
     7861 cctgccgcgc ctgtgcgaac actgcctcaa tcccagctgc gtggcgacct gcccaagcgg
     7921 cgctatctac aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg
     7981 ctggcgtttg tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg
     8041 caagtcagaa aaatatatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg
     8101 ctcagaaacc tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg
     8161 catcgaggaa gcggcgagca ccgagcgcga agttgacctc
//
LOCUS       DAJTYG010000136         8161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTYG010000136 DAJTYG010000000
VERSION     DAJTYG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.136"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(61..600)
                     /locus_tag="OO568_003541"
     CDS             complement(61..600)
                     /locus_tag="OO568_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6642726.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQVWFSLTYQ"
     gene            complement(665..1213)
                     /gene="fimA"
                     /locus_tag="OO568_003542"
     CDS             complement(665..1213)
                     /gene="fimA"
                     /locus_tag="OO568_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6642727.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTINFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="OO568_003543"
     CDS             complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="OO568_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR6642728.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(2768..3370)
                     /gene="fimB"
                     /locus_tag="OO568_003544"
                     /pseudo
     CDS             complement(2768..3370)
                     /gene="fimB"
                     /locus_tag="OO568_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            4824..5540
                     /gene="nanC"
                     /locus_tag="OO568_003545"
     CDS             4824..5540
                     /gene="nanC"
                     /locus_tag="OO568_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6642729.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            5560..6666
                     /gene="nanM"
                     /locus_tag="OO568_003546"
     CDS             5560..6666
                     /gene="nanM"
                     /locus_tag="OO568_003546"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6642730.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            6731..7711
                     /locus_tag="OO568_003547"
     CDS             6731..7711
                     /locus_tag="OO568_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6642731.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTTLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2428 a         1743 c         1697 g         2293 t
ORIGIN      
        1 tacattgacg tttttattac tcactgtcct gttcctgtta tcacattatc tactgaacaa
       61 ttactgatag gttaaagaga accagacctg ggcattggcg atgccgccag taacccgacg
      121 cccggtagca cgatatttgg cgatgaaatg tagcgaagta gagcctgagt aaagccgttt
      181 ccagtttgct ggaggacgat taatcggtac gaggtttcct tcatcatcaa acaacgctac
      241 accaatattg gaggctatcc ctggcccctc tcccacggag agcacatccg gatttttacc
      301 atccgcgaca ccgtgaaacg ccacacccac acgttcactc accaccgtgc tacattcccg
      361 taaatgaata acaaaaggca ccggtgcgct atcttcccct gccgcatgaa accggttact
      421 gctgatttgc cccatattga ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc
      481 cgcaataatg acgccctgaa attgcatatt tccgccgggc agcgtggtat tccatttatt
      541 tccggccaga gcaaacattg gcagcaacga cgctaacaga aataatctct tccttttcat
      601 cgttattttt atcgcacaag ggtgggcatc cctgcccgta atgacgtcct tgaacctggg
      661 taggttattg atactgaacc ttgaaggttg catccgcatt agctgcaccc ggggttgcct
      721 cgccgattgc ataataacgc gcctggaacg gaatggtgtt ggtaccgtta ttcagggttg
      781 tttgtgcact aaatgtcgca ccgtccagcg tcagagcatt gcctgttctg tccaggatct
      841 gcacgccaac gtttgttgca ctacctgcag cagaactctg cagagctagt acatcagtac
      901 gcgtagcatc aattgccgta cctaagaagg caacagcggc ttttgtggca acagtggtat
      961 cgcaatcatt cagctgaatg ttaaaaccaa cggcagagct ggttgctcca gcctgcttca
     1021 ggctagcggt acgaacctgt cccaactgaa cggtttgatc aacagagcct gcatcaactg
     1081 cgcaagcggc gttaacaact tcccctttaa agttaatggt cccaccattt accgtcgtag
     1141 tatcggccag agccgctgcg gaactgaggg acagagccga cagaacaacg attgccagag
     1201 ttttaatttt catgctgctt tcctttaaaa aaactatttc taaatcgaca tgggcagtcg
     1261 ttctgtacac tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa
     1321 aagatgaaac atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt
     1381 cagtttattt gttggcttaa tattctattg ttatctttat ttatagattt ttatattgca
     1441 tgaggtggtt tttggagaga agaatgagga agatgcgtcg agccacagaa acgttagctt
     1501 tacatatagc ggaggtgatg tgaaattaat ttacaagaga aataatttac atatcaaata
     1561 gttagatgct ttttgtcgtt ttttaatatt tttatgcttg agaaaaaata agtaacttat
     1621 ttatgatatg gacagtttgg ccccaattgt cttgtgttta tttgtttttt aactttatta
     1681 tcaataagtt aaatcaaacc tcttctcttt ttaatttttc gtttatgaga ttatttcttt
     1741 cccataatcc ggcaaaacga gcagcattac tggcggtata acgcacagta tggcgaatat
     1801 ttcgatgccc gagataatcc tgaattaaac gggtatctgc accacgctcc gccagttcat
     1861 aaccgcaagc atgccttaac atatgaggat gagtctgcgt tacggttcca gcttcaatac
     1921 cggcatcgcg aataatgcga taggcctgct ggcgagaaag ccgactcccg cggcgagaaa
     1981 taaatatagc gtcggtccgg tcagcgcctt tccagttagc acgttcctgg gtccagcgtt
     2041 ccacggcttc acgctcatca aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc
     2101 ggcgaatatt tattcgacct tcattaaggt caaggtcctg ataatgcaga tcaagcagtt
     2161 cactaatacg catcccatgc cgatatgcca acagaataag acaataatct ctggctcccg
     2221 ttgccccgta acaaaccgcc tgcatcatgg cctgaacttc tttaccggta agataacgac
     2281 gtttactcac aatagtagta ctcctaactg agatatattt gaatgaatac ctataggaaa
     2341 cctcaatcgg tcaaaattag cccaatagat aaaaagataa ccacacacga agttatgtgg
     2401 ctatcagtca attacattaa acatcataca aaaataaaat atcactgatg tgacaacatc
     2461 tattttcatt gatttgattc aatataacaa tagattacag aggattaact tccagttact
     2521 taaaaaataa agaaaaaagt gtggaaacag gagaatttaa gtgatgtgta aacttatgaa
     2581 tgattttgtt tatttatttt gaagaacata aaccattaga ttttgtatgg gtatggttaa
     2641 cttaatgttt attatgataa ttaagaatca cacttacaat aacatcaatt aataaatggt
     2701 taatcatttt tgatatatcg taagaataat gtagttttta acaccatccc tggtatctca
     2761 actatctcta taaaacagca tgacgctgtc gtcctctggc tctatcccag atgccgtaaa
     2821 aacgccctgc attgctggcg gtataccaga cagtatgacg aatattgcga tgcccaagat
     2881 aatcctagat aagtcgcgta tctattccta tattcgccaa agcaaaacca cacgaatagc
     2941 gtaacatgtg cggatgaatc tccagtgaca acccggcatt accaccagaa gtcgagataa
     3001 tatggtaaaa ctgttgccga gaaagcggat tccccttacg tgataaaaat acccactcgc
     3061 tctcagcatg cggataagaa gtacggatac tcaaccagtt ttttaaagcc tgaacttctt
     3121 tgttcaatag cgggtgcgtt gttgaaaagc ctttttttaa tcgatgtata tatatacact
     3181 ttgctttaag atcaatatcc gaaatcctca atcgacaaat ttcactcgcc cggaaaccat
     3241 gaataaaaca aagcaaaatc agacaataat tacgtgttgc atgaggcccg gtatttgctg
     3301 ctttaaggag tgactcgatt tcactatggg taaggaagtt cctttttttg ttatcagcct
     3361 tattcctcat cgttttccct tataattaca gacgcgcact agctgtgctg ggttagcaat
     3421 aatccaacat ttttatcctg attatgttta taaaagcgaa cgtttgctta ataacttacg
     3481 taagtggacc agttcttctg agcgaactta aatggagtag cgatagttac ttataataac
     3541 tttgccatac aaacgccatg ctcttgcatg ctatgtacct ttatatattt atcaatctga
     3601 tacgaatcta ataataaccc tcatcattaa tgcaactaat cttatctata tatcatgtga
     3661 tatgttctgt aacaagtaaa atcaacataa aaaattcagc aaaatttaat atttgttcaa
     3721 ctagccgtct caaatatgac attattagtt aactgcattt gccacaaaat ggctatttca
     3781 tggaacgcag taatttctta gtggctatta tcatgctaat tcttagatcc ctagcgatta
     3841 ttcctgcctg gttcgtcagc ttaggccatg agattgttac attccttttt atcctggaga
     3901 tatgggggaa cgcaggcgac gctttttcat ctacatccgt tggtaaatat agctttggtc
     3961 actacgactt aagccttcct caatcacagc tattcgccgg gcgatacggc aaacaaacta
     4021 agtgaaacat catcctgaaa acaccaacat caacaagcct ctccagatcg acatcaggag
     4081 tgaccagtta caagccacaa accaagaaac atatttcatg atgagaatta tgctcagcct
     4141 gatggcgagt aaacccttat tttcaccact ctcactgagt tagcttgcaa atctttcttt
     4201 gtttagattc taaataaatt atttataaaa catatagtta aaagaaaatc tttagttttt
     4261 gaagaccacc gcaagtgttc gtctggcttc acatgacatc ttcttcttag aaaaagatcg
     4321 acatattttg tgacgcgaat tgcaaatctg gttttgttgt atggattgcg tgatttttga
     4381 tctggtataa caggtataaa gatgcaccac gatagtcaat gagactgggc atctcgtaat
     4441 ctatggcaaa catcacttca gttctttcac atcgggtgat gaaaatgcac ttcagtctga
     4501 aaggaatatg aaaatgagat caacagacac tctattttat gactctgggt aaaatgggtt
     4561 gagtaagtga tataacttac gaatattcaa atcaattaaa aatcagaaga gattttatac
     4621 tcaggtattt aatctggatc tctgtttatt taaataatgt taaaagagaa ttttcacagg
     4681 agaccttata caaaaaatat aaaatacagt tacccgttgc caaagacact ataagcctgg
     4741 caaaaaaata ttacacaaca taaatgctaa ttgtttatgc gggctttgta ttgctttctg
     4801 tatcctacaa atgagtgaaa tttatgaaaa aggctaaaat actttctggc gtattattac
     4861 tgtgcttttc gtccccatta atttctcagg ctgcgacact ggacgtacgt ggtggatatc
     4921 gtagtggaag ccacgcctat gagactcgac tcaaagtcag tgagggatgg caaaatggat
     4981 ggtgggcaag catggaaagt aatacctgga ataccattca tgataataaa aaggaaaatg
     5041 ccgcactcaa tgatgttcag gttgaagtta attacgcaat taaacttgat gatcaatgga
     5101 cggtgcgccc gggaatgtta acgcatttta gcagcaacgg cacacgctac ggaccctacg
     5161 taaaactgtc ctgggacgcg acaaaagatc ttaattttgg cattcgctat cgttacgact
     5221 ggaaagctta ccgacaacaa gacttatccg gtgatatgtc tcgtgataac gttcatcgtt
     5281 gggatggata tgtcacttac catattaata gtgatttcac cttcgcatgg caaacgacgc
     5341 tatacagcaa acagaatgat tatcgctatg caaaccacaa gaaatgggcg acggaaaatg
     5401 cctttgttct acaataccat atgacgcccg atattacgcc atacatagaa tatgactacc
     5461 ttgaccgcca gggtgtttac aacggcagag ataatttatc ggaaaacagt tatcgcattg
     5521 gtgtgtcatt taaactgtag tagacaggag acagtcacaa tgaataaaac tataatggcg
     5581 cttgctatca tgatggcttc atttgccgca aacgcgtctg tattaccgga aactcctgtg
     5641 ccatttaaaa gtggtaccgg agcaattgat aacgacactg tctacattgg tttaggtagc
     5701 gcaggtacag catggtacaa gctagataca caggccaaag ataaaaaatg gactgcgtta
     5761 gctgcattcc ctggtggacc gagagagcaa gcaacctctg catttattga tggcaatctg
     5821 tatgtgtttg gcggcattgg caaaaacagc gagggattga ctcaggtatt taatgacgta
     5881 cacaaataca accccaaaac caatagctgg gttaaattga tgtcgcacgc gccgatgggc
     5941 atggcgggtc atgtgacttt tgtacacaac ggcaaggctt atgttactgg cggtgttaac
     6001 cagaatatct tcaatggcta ttttgaagat ctcaacgagg ctggaaaaga ttcaaccgct
     6061 atagataaaa tcaacgccca ctattttgac aaaaaagcag aagattattt cttcaataag
     6121 tttctgttgt cttttgatcc ctcaacacag caatggagtt acgctggcga atcgccctgg
     6181 tacggaacgg ctggtgcggc ggttgtgaat aaaggtgata aaacctggct tattaatggc
     6241 gaagccaaac caggattgcg aacggatgcc gtatttgaac ttgatttcac cggtaataat
     6301 ttaaaatgga ataagcttga tcccgtctca tcaccagatg gcgtcgctgg cggttttgcg
     6361 gggataagca atgattctct tatatttgcc ggaggggccg gattcaaagg ttcacgagaa
     6421 aattaccaga acggtaagaa ctatgcgcat gaaggcctga aaaaatcata tagcactgat
     6481 attcatcttt ggcataacgg gaaatgggat aaatcgggtg aattatcgca aggtcgggcc
     6541 tacggagtat cattgccctg gaataatagt ttattaatta ttggcggtga aactgcaggc
     6601 ggcaaagcgg tgacggattc agttttaatt tctgtgaagg ataataaagt cacagtacaa
     6661 aactaacgct tcagggcccc ggtaaagggg ccctgctatc aatttgtcat ttgtattacg
     6721 cgaaaattat atgaacgcaa taatatcgcc cgattattac tatgttctta ccgttgctgg
     6781 tcagtctaat gccatggcgt atggcgaagg actgccatta ccggacaggg aagatgcgcc
     6841 tcattccaga attaaacaat tagcgagatt tgcgcatacg catcccggag gcccgccatg
     6901 tcactttaac gacattattc cactgactca ctgcccacac gatgttcagg atatgcaggg
     6961 ttatcaccat cctctggcaa cgaatcatca aacacagtac ggcaccgttg gccaggcact
     7021 gcatattgca cggaaattac tgcccttcat tcctgataat gcagggattc ttatcgttcc
     7081 gtgttgccgt ggcggatcgg cttttaccgc gggcagcgaa gggacatatt cagaacggca
     7141 cggagccagc catgatgctt gtcgttgggg aacggatact acgctgtacc aggatttagt
     7201 cagcagaacg cgagccgcac tggcaaaaaa tccgcagaac aaattcctcg gcgtatgctg
     7261 gatgcaaggc gaatttgact taatgaccag tgactacgcg tcacaccctc aacactttaa
     7321 tcatatggtt gaagcctttc gtagggatct aaaacaatac cattctcagc ttaataatat
     7381 tactgacgcg ccgtggtttt gcggcgatac cacctggtac tggaaagaaa atttcccgca
     7441 ttcgtatgaa gctatttatg gcaattatca aaataatgtt ttagccaata ttattttcgt
     7501 cgacttccag caacaaggtg aaagaggact gacgaacgcg cctgatgaag acccggacga
     7561 tttaagcacg ggatattacg gttcagcgta ccggtcaccg gagaactgga cgacggcact
     7621 gcgaagcagt catttcagca cggcagcccg tcgggggatt atttctgaca gatttgtaga
     7681 agcaattttg cagttttggc gcgaaaggtg agcggggagt ttattcatgg gggatgtcct
     7741 tgttcgttct cagagtagac aacaaaaaac aaatgagatc aaggtaatat ctcttcactt
     7801 gcgggctcga acagcaggca attgccaata taataatctg gcacaagggt gaaaaacgcg
     7861 caccgcataa accgttgtta ttgcttacag attagccgga tgccttaata gacatccgcg
     7921 ccttttcgat tatggctcgg aaatctacga acatctacac agtttactgg aacgttttgg
     7981 gccacagcgt tcgcagcacc gtcctgatat gccgttctgg cgattataag tcgacggatt
     8041 ctggcaaata gacgatgatg atatgcttaa tgatgttcaa agaaattagg ccattattat
     8101 attattgctt gcattttctt tcattcctaa aagcacccgc cattaatccc aggcgcatta
     8161 t
//
LOCUS       DAJTYG010000137         8093 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTYG010000137 DAJTYG010000000
VERSION     DAJTYG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.137"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..893)
                     /locus_tag="OO568_003548"
     CDS             complement(<1..893)
                     /locus_tag="OO568_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR6642732.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPS
                     SVNVLSELPGRTVPYEAAEIRPQVGGIIIKRNFIEGDKVNQSDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSR"
     gene            953..1231
                     /locus_tag="OO568_003549"
     CDS             953..1231
                     /locus_tag="OO568_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642733.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1232..1759)
                     /gene="gadE"
                     /locus_tag="OO568_003550"
     CDS             complement(1232..1759)
                     /gene="gadE"
                     /locus_tag="OO568_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6642734.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2558..3130)
                     /gene="hdeD"
                     /locus_tag="OO568_003551"
     CDS             complement(2558..3130)
                     /gene="hdeD"
                     /locus_tag="OO568_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6642735.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3385..3717
                     /gene="hdeA"
                     /locus_tag="OO568_003552"
     CDS             3385..3717
                     /gene="hdeA"
                     /locus_tag="OO568_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6642736.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3833..4159
                     /gene="hdeB"
                     /locus_tag="OO568_003553"
     CDS             3833..4159
                     /gene="hdeB"
                     /locus_tag="OO568_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6642737.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4223..4869
                     /locus_tag="OO568_003554"
                     /pseudo
     CDS             4223..4869
                     /locus_tag="OO568_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4911..5441)
                     /locus_tag="OO568_003555"
     CDS             complement(4911..5441)
                     /locus_tag="OO568_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6642738.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5597..6163)
                     /gene="slp"
                     /locus_tag="OO568_003556"
     CDS             complement(5597..6163)
                     /gene="slp"
                     /locus_tag="OO568_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6642739.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGLGAPYYTNAVSQVTPELVK"
     gene            complement(6410..7633)
                     /locus_tag="OO568_003557"
     CDS             complement(6410..7633)
                     /locus_tag="OO568_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642740.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGKDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
BASE COUNT         2351 a         1571 c         1696 g         2475 t
ORIGIN      
        1 cggctacctt catccactaa tgccgtgaca tacatgccag gcagcaagtc accatttggg
       61 ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catcaaccgt cgggtcggag
      121 aatttcagcg tgccggtctg gctgtaacgt ttaccatttt ccagattgag ctgtactggc
      181 gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat gcgtaagaaa
      241 tcttgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac ggtaaccagc
      301 gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt cccgctgacg
      361 cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg ttcaacagcc
      421 gctttggcga cggtgacatt ggcttctgct tcattcagct gggtgcgcgc ggtgtcgtaa
      481 tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa ggtgatgcgg
      541 gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt tagctcggcc
      601 tgtaaaggtg caggatcaat ctgatacagc gaatcgctct ggttcacttt atcgccttcg
      661 ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc agcttcataa
      721 ggaacggttc taccgggcaa ttcgctcaac acattgaccg aactagggga gagtgtgacg
      781 acaccgacct caggcgtcat ggcggcggcg ttttccgccg atttgtcatc gcaggcggtg
      841 agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt cattttagtc
      901 cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag atatgaggga
      961 tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca ggcacagtga
     1021 tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag ttcctgccag
     1081 cattcgggca acgaagggtt ctactggtgg atacacatac caggggaatc aagtcgtttt
     1141 agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc ctcgtcatgc
     1201 cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata cccagggtga
     1261 cgatgtcgct catacgtctt agtttcagct tgtacataat gttgaatttc tgaatcttta
     1321 atgtttgttg cgaaatattg agcgtcgacg tgatattgct ttcattttta tttttcagaa
     1381 tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc ttatggggca
     1441 agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg ctaacaaaat
     1501 aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca ataattctct
     1561 cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga ttgtgatagg
     1621 tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac agggaattga
     1681 tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga aaagaatctt
     1741 tcgtcatgag aaaaatcata acttgctcct tagccgttat tgtttcgaac atgtcatcct
     1801 tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc tataagaact
     1861 ttgcccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat ttatccatca
     1921 cgcactccat atattgatcc taagtaagat tcctggttgt tatcagcttg taaatagtaa
     1981 acaaaaagtt aacgtttgcg gattactgcc caagaataag ttatcaataa ccagtttgtg
     2041 atctctgaag aatattacta aagttaaaat gtaatccgat ttaaatatcg agtctccttg
     2101 tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga aattacctga
     2161 gtaaataacg catttatgtc ttcaggtttt tatatagcgt ctggagacac ggatatttat
     2221 gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt aaccgcccat
     2281 agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa aacattaaac
     2341 ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc catggagtga
     2401 aaaaaattag atgaaattca gtaggttgaa ataatcacta gcaggtaatt atttcaatga
     2461 tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg atcatagtca
     2521 catcaagtga ctatgatccg ggtgacaacc ggggtaatta ttgctgctta acgaacaaac
     2581 tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc agtgtggaaa
     2641 ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg acgatatcca
     2701 gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga taccaactca
     2761 tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca atggcaaaaa
     2821 tgcccagctc cggtgcacgg atgaagaaat agccgatcaa taaataggcg actgcgacga
     2881 ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataac ccgacaataa
     2941 gcgcaatacc cgagcagatt aataatgcac ccactactgt gcttaaaata tcgccagaga
     3001 cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca ataaactgga
     3061 ttgctctgcg atgctttttc agcatctcca gatcaaactt caaaattgtt gccttatcta
     3121 tatataacat agaaccaccc tataaaatta agaagaaaat cccctgctat caatctatgc
     3181 caaaaacgcg tctaagaatg cagtcgattt aataaaaatt tcctaattgc agtatctgat
     3241 gcatctgtaa ctcattgtat tgaaataaaa atatctgatt ttgatatttt ccatcaacat
     3301 gacatataca gaaaaccagg ttataacctc agtgtcgaaa ttgattcgtg acggctcttt
     3361 cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct tggtggtctg
     3421 cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga taacaaaaaa
     3481 ccggtcaact catggacctg tgaagatttc ctggctgtgg acgaatcctt ccagccaact
     3541 gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc ggttttagat
     3601 gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca ggataaacaa
     3661 gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga tatgtaattc
     3721 cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat cttctttaaa
     3781 agcagctatt cctcctgttc atatataatc tctatattga atgggttaca aaatgaatat
     3841 ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt cactggtgaa
     3901 cgcacaatct gcgctggcag ccaatgaatc cgctaaagat atgacctgcc aggaatttat
     3961 tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg aagaaacagt
     4021 atataaaggt ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa ttcctaaagt
     4081 gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa atcaagcatc
     4141 taatgacttg ccgaattaat gaggtacaag taaaaaggag tagcaagttg agccatcttg
     4201 ctgctccttt ttgcattttt atatgacagc agaatttatt atacgtctta tactcgcggc
     4261 aattgcctgt ggcgctattg gcatggaaag gcaaatgcgc ggcaaaggag cagggttacg
     4321 cacacatgta ttaattggca tgggaagcgc cctgtttatg attgtttcga aatatggttt
     4381 tgctgacgtg ctgtctttag atcacgtcgg actcgacccc agccgtattg ctgctcaggt
     4441 ggtgacgggc gtcgggttta tcggtgcagg taacattctc gttcgtaacc aaaatattgt
     4501 aggtctgacg acggcagcgg atatctgggt gaccgccgca taggtatggt tattggcagc
     4561 ggtatgtacg aactgggtat ttatggttca gtgatgacct tgttggtgct ggaagtcttc
     4621 catcaattaa ccttccgcct gatgaacaaa aattaccatt tacagctgac attagtgaac
     4681 ggtaatacgg tatcaatgct cgactggttt aagcagcaaa aaataaaaac tgatttagtt
     4741 tcgttgcagg aaaatgaaga tcatgaagtg gtcgccattg atatccagct tcatgccaca
     4801 acgttggtag aagacctgtt gcgattatta aaaggtatgg caggggtgaa aggcgtttca
     4861 attagctaat ctgtgtggca gcgtagccag actcaccgta agcctgaaat tcacaccaga
     4921 taatcaatat gctgatggcg taccagctca ttgatccgct taagattgag gatcatcatg
     4981 atattgtgtt tatggcagta gaacgttttt agcgatattt tcagtttctc cagaatatct
     5041 tcagttgtca tttgattcgc aatgcagctc agaacctgat gttgcttgcg cgaaagtgac
     5101 acattcgctt caggcacaga gcagtaactg ccattgatga gtgcgacaaa atcaatgata
     5161 gtgatagtcc gcggaacaaa ggtcaccggt actttcccca gacagcgttt aatattaaag
     5221 ggggagaaaa caatgacatg gacaggtttt aatcgttcaa tttgagtgag aaaattagaa
     5281 tgaaatacat tattcattaa tgtatcaata atgacgaagg cattctgatg caacattacg
     5341 tcgatctctt cttcgttctg tatatgaacg acattacctt tactcagaat gtttttcatc
     5401 gcggtgaaga acatcgtatc cctggtaatt ataagaaaca taaccgactc ctggtgcgga
     5461 cattaacttt ataatcagtt tgccaaaggt aattaacagg acagggctta taataataat
     5521 agttagctta ctgtatatta attttatgta atacatgatg cagatgctga gaaagcatct
     5581 gcatctttcg gtggtgttat ttgaccagct caggtgttac ctgactcacc gcattggtgt
     5641 agtaaggcgc acccaagccc ggttccggcc agaatgcacc atagccgtaa tcccacaggt
     5701 tataggtggt attaaccact tctctcaaat gccacacctg gatgccctgc atattcactt
     5761 ccaggaagtt atacgggact ttattgataa agcctggttg ttcaccctga atggtaccga
     5821 ggatggtaac aaagtgatta cgatagttca ctggatcaag gaagccgctt tgtctggcga
     5881 gcagtcggcc ctgatagttg gcttcaatat caggcttcgc atagctatcc aacggtaata
     5941 cagcgatttc taacaacgta tccgttttgc cattgataac gttgataacc ttcccaccaa
     6001 agcgcgcttg ttgaccaaca tataaccccg gctggttatg aacagcaaca aaactttttt
     6061 gaatatcagg ttggttattg cctttgatat tttgcggaat tgaactacat gcggcaagca
     6121 aaaatgaaag gctgaggatg agtgcacctt ttgtcatgtt catgttacta tccttatcaa
     6181 caaatattaa aaccataata gatatttctt tatagttttc atctgattct gaggtgatat
     6241 gcctgatagt aacgtaaacc tgtctgatgt ttatatccct tctgtttagt tcaattccgt
     6301 tattttgggg cgtggtatga gtatccatcg cccaataata taaccctttg ttttaaaatt
     6361 acataaaatc tatttttatt caataattat aaagtgctaa taatcaatat tatggtatta
     6421 tattctgata atgatttgat aagcgaattc ttttgccaga tggtatttct atttccaccc
     6481 cataatttat atctttttca ttaggcagat ctctctttaa ataaaagggg tatgaaatct
     6541 tttcgggttg aagtgtctcg taatgaaatg ctatgaattc atttttagac attgttgccc
     6601 atttttgaat tttattgtat agagagggtg ttgtaaactc ttgaagttgt ttataacata
     6661 tttttttgaa ctcttgctta tattttaata tgtcatcaaa tgaaagattc actacctttg
     6721 cctctaagaa atcaattatt ctcgaagcaa tttccgcttt cgcgcgtaaa gagttgtcat
     6781 taatagttaa caatgtagaa aacaagccat tgatttgttt gtcaagatgt tgttcatatt
     6841 tatgtagttt ggggatggga ttttccttgt gtgaaaaaat agtgttaaat aaatttgcgt
     6901 cagtgaaatt tatattaagt agatcttcaa aataaaattt tgataaggtt aggactagct
     6961 tggacatatc tttccctgtc atgttgaaat caataaagtc gcatccagca agggcttcca
     7021 tcctattttc atttttactg tgctcaatat acagcttcgc catttttaag tacaagcttt
     7081 gaatgtctct tatatcggtt ggtaagtcag ctcgttgccc agcaaaattt ttattatcgg
     7141 aagaaacggt ttggacactg aatctttcgg gtattccttc ttctacgaga attttagtta
     7201 tgattggatt gttgggttct ggattaacga caacaatgat ttgacataca gaatccttat
     7261 ccttttcgta tctaatgtta tgaatctgat taagtattga ttgtgtctgt tgcgtaagtg
     7321 gtgtcgaact tatcgtttca atgttattaa taagacttac aagttgaacc acaacttttg
     7381 agtagtctgc atttgtatat gtggtgttcg tgtggcatgc attaatttgt tggagttgtt
     7441 gttgcgctaa atcaaattgt tcagggttgt atccttctcc agagaaggtg gaattaatgg
     7501 gttgttgttc ttcccaatta tcacaaggcc acttcggtgt gtctggcgtt ttaacattat
     7561 cggtatttgt tgtgcagtta taaatatcgc cgtgatatgt atttattata cctggggtaa
     7621 agtcaattga cataacattc cttattcatc atctgtttta tgctaatgtt tttaaattgg
     7681 ataattagaa caggaatgga ttttcttcta cacacaataa atataatgcg ttatatcttc
     7741 ctgatataat tacctttaaa ttccctttca tttccacccg gtaatatttg tgtataaaaa
     7801 aagggcattg atagtaacgt aaaggaaaac gttaacaact atcaaaaagc gctcatttta
     7861 aaaagttatt atgttctccg atagtgtgtg aagtaatatt tttgtgatgt tttagatgtg
     7921 taataatgaa ccaggttaaa gtgtatttaa aaatgtgttg tattagcgac tgaaatctgc
     7981 cttttaaaaa ggaggagggt actgagacaa agagtcattg cttctccgat aaaatgtcag
     8041 agaagcatga ttacgtggtt attgtctgtt gatgacttgt tgaccgagcc gtc
//
LOCUS       DAJTYG010000138         7799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTYG010000138 DAJTYG010000000
VERSION     DAJTYG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.138"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            93..2273
                     /locus_tag="OO568_003558"
     CDS             93..2273
                     /locus_tag="OO568_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6642741.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNHQM"
     gene            complement(2414..3697)
                     /locus_tag="OO568_003559"
     CDS             complement(2414..3697)
                     /locus_tag="OO568_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6642742.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3832..4902)
                     /locus_tag="OO568_003560"
     CDS             complement(3832..4902)
                     /locus_tag="OO568_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6642743.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKIFASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(4880..5098)
                     /locus_tag="OO568_003561"
     CDS             complement(4880..5098)
                     /locus_tag="OO568_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR6642744.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(5183..5806)
                     /locus_tag="OO568_003562"
     CDS             complement(5183..5806)
                     /locus_tag="OO568_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCR6642745.1"
                     /translation="MTSQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            complement(6128..6835)
                     /locus_tag="OO568_003563"
     CDS             complement(6128..6835)
                     /locus_tag="OO568_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6642746.1"
                     /translation="MAQLYGTDVVRIRQNHHENKSRFVEGVHFYKVVGDELRALKHKV
                     ALNYFVKSAPNARHLILWTERGAARHAKMLETDQAWDVFEKLEDGYFNQREKVANHPQ
                     PVPQPESSLDRARAMEIALNVADRLVNMFLNLSSESKQAAVAGVFNPIVGRDVVPLPA
                     ISEHYYSATEVGQRYGISANKVGRIANTYMLKTEKYGKWFIDKSAHNDKQVETFRYNE
                     AGVHKIEELIEGERKAA"
     gene            complement(6783..7217)
                     /locus_tag="OO568_003564"
     CDS             complement(6783..7217)
                     /locus_tag="OO568_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6642747.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIGAVVRNGCCAHPAKPS"
     gene            complement(7290..7616)
                     /locus_tag="OO568_003565"
     CDS             complement(7290..7616)
                     /locus_tag="OO568_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR6642748.1"
                     /translation="MPLHVQAYANAEFIAAFNPNVALALLDERERNLQYIKSRDQENE
                     EIALTVGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRIAELEAQTVTV
                     KEVGDA"
     gene            7678..>7799
                     /locus_tag="OO568_003566"
     CDS             7678..>7799
                     /locus_tag="OO568_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6642749.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMT"
BASE COUNT         1828 a         1940 c         1903 g         2128 t
ORIGIN      
        1 cttgtcgcct tcctgcaact cgaattattt agagtatatc catttattat ctttctgcgc
       61 acttcacggt gcgcagatat ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt
      121 ttctcgccag taataacgaa ataattgccg aagaacattt caccggcgaa attaaaaaag
      181 aagaagcaaa aaaaggcact attgcctggt ctattctctc ttctcataat acgtccggaa
      241 atatggataa acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg
      301 gtattgtaca gaccgctaaa gcgtccggaa tggaacgttt cccgctgccg tatgtgctga
      361 ccaactgcca taactcactc tgcgccgtcg gcggcaccat taacggtgat gaccatgttt
      421 ttggtttatc ggcagctcag cgttatggcg gtatttttgt gcctccgcat attgcggtca
      481 tccatcaata tatgcgtgag atgatggctg gcgggggcaa aatgatcctc gggtcagaca
      541 gccacacccg ttacggtgca ttagggacaa tggcagtcgg tgagggtggc ggtgagttgg
      601 taaaacagct gcttaatgac acctgggata tcgactatcc gggcgtggtt gcggtgcatc
      661 tgaccggaaa acctgcgccg tatgtggggc cacaggatgt ggcgctggct atcattggcg
      721 cggtgttcaa aaacggttac gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta
      781 gcgcgctctc taccgatttc cgtaacagcg ttgacgtgat gaccacggaa accacctgtt
      841 taagctccgt ctggcaaact gatgaagaag tgcataactg gctggcgctg cacggtcgcg
      901 gccaggatta ctgccagctt aaccctcaac cgatggcgta ctacgatggc tgcatcagcg
      961 ttgatttaag cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg
     1021 aaatcgacac actgaaccag aacctgaccg acattctgcg tgagattgaa attgagtccg
     1081 aacgcgtggc gcacggtaaa gccaaactct cgctgctgga taaagtggaa aatggtcgcc
     1141 tgaaagtgca gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg
     1201 cggcggcgaa tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcggttt
     1261 acccgtcatc acagccggtg tttatggatc tagccaaaaa aggtgtggta gcagatttga
     1321 ttggcgcagg cgcaatcatc agaaccgcat tctgtggccc atgctttggc gcgggcgata
     1381 cgccaatcaa caacggtttg agtattcgcc acaccacgcg taacttcccg aaccgcgaag
     1441 gctctaagcc agccaatggg cagatgtcag cggtggcgct gatggacgct cgttctatcg
     1501 ctgcgactgc ggcaaacggt ggctatttaa cctctgccag cgaacttgat tgctgggaca
     1561 acgtgccgga gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg
     1621 tgaaaggggc aactcagcaa ccgctgattt acggaccgaa cattaaagac tggccggaat
     1681 tgggtgcgct gactgacaat atcgtcctga aagtgtgctc gaagatcctc gacgaagtca
     1741 ccaccaccga cgaactgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg
     1801 gtctggcggg gaatgtcagc gagctgacag aggtgtttgc gcgcattaag cagattgctg
     1861 gtcaggagca tattgatccg ctgcaaactg aaattggcag catggtctat gcggtgaaac
     1921 caggcgatgg ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt ggcggtctgg
     1981 cgaatattgc cgaggagtac gcgactaaac gctaccgttc taacgtcatc aactggggga
     2041 tgttaccgct gcagatggcg gaagtgccaa cctttgaagt gggggattac atttacatcc
     2101 ctggcattaa agcggcgctg gataatccgg gtacgacgtt taaaggttat gtgatccatg
     2161 aagatgcgcc ggtaacggaa attacgcttt acatggaaag cctgacggct gaagagcgcg
     2221 agattatcaa ggcgggtagt ttgattaact tcaataaaaa ccatcagatg taaaaagcgc
     2281 catgtgaatg taggtcgcat tcggcactta ttgtcggatg cgatgctggc gcatcttatc
     2341 cgacctacaa atcgcatcga accgtaggcc ggataaggcg tttacgccgc atccggcaaa
     2401 tagttaatag ctcttacttc ttcgcctctg caaccacttt gctgcccacg ccgcggttat
     2461 tgtattccca catgcggttg tagttagtgt cattcagatt gcgctgtatt tcgtcgttat
     2521 catctacgct gccggtatta cccgcaaacg gacgattaga gatcaccgca tcggcccacg
     2581 gtttagccgt gttaaaacct tcgttgatgg cgctatcacg gatcaccacc tgaccgttgg
     2641 tattagcatc aacatccagc gagcggccca gttgcgccac gccatcaccg gaagcattga
     2701 aacggctgtt tacggcgagg aaaccgtaat agatgttaga cagcgtagcc ggtgcaaaca
     2761 catatgcttc ttgctgggta cgtgagttca ccacgcggaa ttcggtgtta tcgaacacca
     2821 ctgcgccgcg accagaaacg atatccacat ccccttcaat atagctgttg gtcaccagcg
     2881 tacgcggctg acggttggtt tccagacggt tctgcacacc gctgttggtg acaaagaagg
     2941 tgttctgacg accgagaatg ttaacgttgt taatctgtac ctggtcaccg tcagtacgca
     3001 gtgctaccgc cggatggtta cctgcatcta cactatcgcc cagcgtgttt tcgatggtca
     3061 gattttgcag ttgcagtcca ttgttttgtg accagaagac cgcagaacac agaacaccga
     3121 tactgtcgct gcgtttgctc tggcagctat cgtacatata ccacgctggt ttacctggca
     3181 tatatttgcc gcgcgggttg acgtcgtgac gccagtcggc agggctcatg cccccatcaa
     3241 gggaaagccc aatcttcaca tcaatcggtt tttcacctgt accgtacaga gtgattccac
     3301 ctggagcagc agggacatac accgttccct gatactcacc aggcatcacg gcaatatact
     3361 ggcgcttgtt ggtgcgcttg ataattgccg catctaccgc cgcctgaatc gtggtatgcg
     3421 ttacaccttg agtacccgcc gggccgacaa caaagtcagg ttgcgcaggc agggttatcg
     3481 gggacggatt ccacgctgca gcacctggtg tcagggatgc aaaatagtgt tgcgcatcga
     3541 aattcagcgc ttcttttgcc gacagaatcg ggcgagaaga ggtaccaggc gcagtttgat
     3601 cagaaggacg ttgatcgggt ggtgttgagc tacaggcggt cagcgtcacg ccaaaagcca
     3661 atgccagcgc cagacgggaa actgaaaatg tgttcacagg ttgctccggg ctatgaaata
     3721 gaaaaatgaa tccgttgaag cctgcttttt tatactaagt tggcattata aaaaagcatt
     3781 gcttatcaat ttgttgcaac gaacaggtca ttatcagtca aaataaaatc attatttgat
     3841 ttcaattttg tcccactccc tgcctctgtc atcacgatac tgtgatgcca tggtgtccga
     3901 cttgtgcccg agaagatgtt gagcaaactt atcgcttatc tgcttctcat agagtcttgc
     3961 agacaaactg cgcaactcgt gaaaggtagg tggatcccct tcgaaggaaa gacctgatgc
     4021 ttttcgtgcg cgcataaaat accttgatac tgtgccggat gaaagcggtt cacgacgagt
     4081 agatgcaatt atggtttctc cgccaagaat ctctttgcat ttatcaagtg tttccttcat
     4141 tgatatcccg agagcatcaa catgcaatgt tgtagggatg gcaattttta cgcctgtttt
     4201 gctttgctcg acataaagat atccatctac gatatcagac cacttcattt cgcataaatc
     4261 accaactcgt tgcccggtaa caacagccag ttccattgca agtctgagcc aacatggtga
     4321 tgattctgct gcttgataaa ttttcaggta ttcgtcagcc gtaagtcttg atctccttac
     4381 ctctgatttt gctgcgcgag tggcagcgac cgggtttgtt gttatatggc cttcagctat
     4441 tgcctctcgg aatgcatcgc tcagtgttga tctgattaac ttggctgatg ccgccttgcc
     4501 ctcgtctatg tatccattga gcattgccgc aatttctttt gtggtgatgt cttcaagtgg
     4561 agcatcaggc agccccctcc ttattgcttt aattttgctc atgtaattta tgagtgtctt
     4621 ctgcttgatt cctctgctgg cgaagatttt ttcgtagcga tcaagccatg aatgtaacgt
     4681 aacagaatta tcactgttga ttctcgctgt cagaggcttg tgtttgtgtc ctgaaaataa
     4741 ctcaatgttg gcctgtatag cttcagtgat tgcgattcgc ctgtctcggc ctaatccaaa
     4801 ctctttaccc gtccttgggt ccctgtagca gtaatatcca ttgtttctta tataaaggtt
     4861 agggggtaaa tcccggcgct catgacttcg ccttcttccc atttctgatc ctcttcaaaa
     4921 ggctacctgt tactggtcga tttaagtcaa cctttaccgc tgattcgtgg aacaaatact
     4981 ctcttccatc cttaaccgga ggagggaata tcctgcactc gcgtacccat cgacgaactg
     5041 tttcaaggct ccttgggcgt cgctggcgtg cgttccactc ctgaagtgtc aagtacatcg
     5101 caaagtctcc gcaattactc gcaagggagc gagtattggt ttattttctc caacaaaaaa
     5161 ggagccgaag ctcctttgat gattaaaatt cgaattgtct cgcccgaagg cttttcagca
     5221 ttggttttgc ccgttcgaaa agggcgcttg cttggtcaag tcgtgtcgcc tcccttagca
     5281 gtacatctct gtttttcgtc accgtgtaaa aagtttcaaa cgcgatgtca tacagcttgc
     5341 tcgtgtatga tgagttcagt tccttcaata tcgggtacag acgtttgcag atgtcctgcg
     5401 cattctccat ctgtacctgc atatagcaaa ggaggatgat ttcttcgtct gtgaattgct
     5461 gcgctggttg catgctgcga agtttctttt cgcactcgat gaagtatcgg cgtatctggc
     5521 ggcctttttc gttacgctcg accatcgccg tttctttggc tgtatcgagg gtgaggtgat
     5581 agtctttgct acggcgatcg cctccacgtc ctttgatccc cctgttttgg tgaaccaaaa
     5641 tgtaatcgac atcaacggca aagccgtatt ctgttatgcg atttttaatc catgtagaga
     5701 agtcacgacg tacttccaaa aattcgtgta aatcacgggc attaacgaga agagttgttt
     5761 cgttggatat agtgccgttg aatacgggga tgagttgact ggtcattttt atgtcctttg
     5821 catttgttca gatagccctg tgttcagcag ggcggtcagg tacttgaaca ccgtgcaaag
     5881 tcggcccgta tccttaacct ttcggctgtt tttcggtata cgcgctaccc gaccatatct
     5941 gaaaaatgga cataaaaaat ccgcatgact gacgggtgcg gtttccgctt tgcaaggtgt
     6001 gttcagcacc atgaagcgga atatagcccc gttaatgcgg tgttgtcaaa tcatgtaggc
     6061 cttatcttgc tgtaagccgc gccattcgtg cttttcccca catttgggga aaactatctt
     6121 gtcaaaatca tgcagccttt cgctctcctt cgatgagttc ctctattttg tgcactccag
     6181 cctcgttgta ccgaaatgtt tcaacctgct tgtcattgtg cgccgatttg tcgatgaacc
     6241 acttcccgta cttttctgtt ttgagcatgt aggtgttggc tatgcgtcct accttgttgg
     6301 ctgaaatacc gtagcgctgg ccgacttccg tagcagagta gtagtgctct gagatagctg
     6361 gcagaggtac aacatcgcga ccaacaatcg ggttgaacac tccagctact gctgcctgct
     6421 ttgattctga ggacagattc aggaacatat tcaccagtcg atccgccacg ttcaaagcta
     6481 tttccatggc tcgcgcacgg tctaggctac tttctggttg cggtactggt tgaggatggt
     6541 tggcaacctt ttcgcgctga ttgaagtagc cgtcctccag tttttcgaac acgtcccatg
     6601 cctgatccgt ttcgagcatc ttcgcgtgac gggcggctcc gcgctctgtc cagaggatga
     6661 ggtggcgtgc atttggggca cttttcacaa agtagtttaa agctactttg tgttttagcg
     6721 cgcgcagctc gtcaccaacg accttgtaga agtgtacccc ctcaacaaag cgcgacttgt
     6781 tttcatgatg gttttgccgg atgcgcacaa catccgttcc gtacaactgc gccaatgttt
     6841 ggtgggtagt tgtttttaaa gtcagctgct aacacatagc attgtcgctc tcccatttct
     6901 ggcattcgct cactacagct tatccaggca tccggagtta ccggcactgg ctgggcggta
     6961 taaagcggtg ttatatctgc ccgaaaatta catgctttat gcagccgcac ccaccgttcg
     7021 acttctgctt tgtcagaata cataccagtg aacgtgttat attcacggtc aatttgcgtg
     7081 aaggttacct tccacgccac cggctctgct gtcagcgatg ccagagcaat ttcataagca
     7141 cggcgttcaa cattgtctcg cacatccaaa ctgctgatac gctctttgat ttctttaatc
     7201 agttctttgt cgttcactta tgcctccgat tcccggatgt cacccaaatc aacgctacaa
     7261 tgccaaccag tgcaacgagc gcaaaactat caagcatctc caacctcttt aacggtaact
     7321 gtctgtgcct ccagttctgc tatgcgcttt tctgaggctt caagtaacgc ctgcttatcg
     7381 cgtagcgctt cttccagttc agcaacatgg cattcactat caataaggtt gttctctgct
     7441 gcttccagct caacacgcaa cttccctacc gttagcgcaa tttcctcgtt ctcctgatcg
     7501 cggcttttga tgtattgcag gtttctttcc cgttcatcca gtagtgccag cgcaacattt
     7561 ggattaaaag cagcaataaa ttcagcgttt gcataagcct gaacatgaag tggcacactg
     7621 aatttggcca cctgaacaga ggtgatatgc tcacctcaga acaacacagg tgctccaatg
     7681 aaaaaaagaa attttagcgc agagtttaaa cgcgaatccg ctcaactggt tgttgaccag
     7741 aaatacacgg tggcagatgc cgccaaagct atggatgttg gcctttccac aatgacaag
//
LOCUS       DAJTYG010000139         7753 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTYG010000139 DAJTYG010000000
VERSION     DAJTYG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.139"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..1153
                     /gene="rbsA"
                     /locus_tag="OO568_003567"
     CDS             <1..1153
                     /gene="rbsA"
                     /locus_tag="OO568_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCR6642750.1"
                     /translation="YVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKV
                     IIMDEPTDALTDTETESLFRVIRELKSQGHGIVYISHRMKEIFEICDDVTVFRDGQFI
                     AEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLRKG
                     EILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISEDR
                     KRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIG
                     LLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVS
                     SEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAVVGKLNRVNQE"
     gene            1158..2123
                     /gene="rbsC"
                     /locus_tag="OO568_003568"
     CDS             1158..2123
                     /gene="rbsC"
                     /locus_tag="OO568_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6642751.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2148..3025
                     /gene="rbsB"
                     /locus_tag="OO568_003569"
                     /pseudo
     CDS             2148..3025
                     /gene="rbsB"
                     /locus_tag="OO568_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3151..4080
                     /gene="rbsK"
                     /locus_tag="OO568_003570"
     CDS             3151..4080
                     /gene="rbsK"
                     /locus_tag="OO568_003570"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6642752.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4084..5076
                     /gene="rbsR"
                     /locus_tag="OO568_003571"
     CDS             4084..5076
                     /gene="rbsR"
                     /locus_tag="OO568_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6642753.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5042..6469)
                     /gene="mdtD"
                     /locus_tag="OO568_003572"
     CDS             complement(5042..6469)
                     /gene="mdtD"
                     /locus_tag="OO568_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6642754.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     NVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6492..7184)
                     /locus_tag="OO568_003573"
     CDS             complement(6492..7184)
                     /locus_tag="OO568_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6642755.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDIAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            7665..>7753
                     /locus_tag="OO568_003574"
     rRNA            7665..>7753
                     /locus_tag="OO568_003574"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1989 a         1866 c         2036 g         1862 t
ORIGIN      
        1 gtatgtcgaa gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagtt
       61 agtgggcgat ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt
      121 tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga
      181 atccctgttc cgcgtcatcc gcgagctgaa atcgcaaggc cacggtattg tctatatctc
      241 ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgatgggca
      301 atttattgct gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt
      361 gggccgcaag ctggaagatc aatatccaca cctggacaaa gcgccaggag atatccgcct
      421 gaaagtcgat aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg
      481 cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct
      541 ctacggcgca ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac
      601 ccgttcaccg caggatggcc tggcaaacgg cattgtgtat atctccgaag accgtaaacg
      661 tgacggttta gtgctgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta
      721 cttcagccgc gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt
      781 cattcgtctg tttaatgtga aaacgccgtc gatggaacag gcaattggtc tgctttccgg
      841 tggcaatcag caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtattgat
      901 ccttgatgag cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat
      961 taaccagttc aaagccgatg gcttgagtat cattctggtg tcatcggaga tgccagaagt
     1021 attaggcatg agcgatcgca tcatcgtcat gcatgaaggg catctcagcg gggaatttac
     1081 tcgtgagcag gccacccagg aagtgttaat ggctgccgtt gtgggcaagc ttaatcgcgt
     1141 gaatcaggag taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag
     1201 cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga
     1261 cgttaagccc gaactttttc accatcaata acttattcaa tattctccag caaacttcgg
     1321 tgaacgccat tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atagacttgt
     1381 cggtaggttc tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag
     1441 tcaatgcgct ggtggctgtc gctgctgctc tcacgttagg tgccgcaatt ggtgcggtaa
     1501 ccggggtgat tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc
     1561 ttttactgcg cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta
     1621 ctgagaacgc cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc
     1681 cagtctggat catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc
     1741 tggggcgtta catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca
     1801 acgtcaataa aatcaaaatc atcgtctatt ctctttgtgg tctgctggca tcgctggccg
     1861 ggatcattga agtggcgcgt ctctcctctg cacaacccac ggcggggact ggctatgagc
     1921 tggatgctat tgctgcggtg gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca
     1981 ttgttgggac gttgatcggc gcattaattc ttggcttcct taataatgga ttgaatttgt
     2041 taggtgtttc ctcctattac cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc
     2101 tggtagacaa caaaaagcag taataacgac tacaggacat cttgaatatg aacatgaaaa
     2161 aactggctac cctggtttcc gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg
     2221 caaaagacac catcgcgctg gtggtctcca cgcttaacaa cccgttcttt gtatcgctga
     2281 aagatggcgc gcagaaagag gcggataaac ttggctataa cctggtggtg ctggactccc
     2341 agaacaaccc ggcgaaagag ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa
     2401 ttctgctgat taacccgacc gactccgacg cagtgggtaa tgctgtgaag atggctaacc
     2461 aggcgaacat tccggttatc actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc
     2521 acattgcatc tgataacgta ctgggcggca aaatcgccgg tgattacatc gcgaagaaag
     2581 cgggtgaagg tgccaaagtt atcgagctgc aaggcattgc cggtacatcc gcagcccgtg
     2641 aacgtggcga aggcttccag caggccgttg ctgctcacaa gtttaatgtt cttgccagcc
     2701 agcagatttt gatcgcacca aaggtttgaa cgtaatgcag aacctgttga ccgctcatcc
     2761 ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc tgcaaactgc
     2821 cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca ccggatggcg aaaaagcggt
     2881 gaatgatggc aaactagcag cgactatcgc tcagctaccc gatcagattg gcgcgaaagg
     2941 cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt caggctaagt atccggttga
     3001 tctgaaactg gttgttaagc agtagtttta atcaggttgt atgacctgat ggtgacataa
     3061 atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc agggcacgcg ccaccctaac
     3121 acggtggcgc attttatgga catcccgaat atgcaaaacg caggcagcct cgttgttctt
     3181 ggcagcatta atgctgacca cattcttaat cttcaatctt ttcctactcc aggcgaaacc
     3241 gtaaccggta accactatca ggttgcattt ggcggcaaag gcgcgaatca ggctgtggct
     3301 gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta ctggcgatga cagcattggc
     3361 gagagcgttc gccagcagct cgccactgat aacatcgata ttactccggt cagcgtgatc
     3421 aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg gcgaaggtga gaatgtcatc
     3481 ggtattcatg ccggcgctaa tgctgctctt tccccatcgc tggtggaagc gcaacgtgag
     3541 cgtattgcca acgcgtcagc gttattaatg cagctggaat caccactcga aagtgtgatg
     3601 gcagcggcga aaatcgccca tcaaaataag actatcgttg cgcttaaccc ggctccggct
     3661 cgcgaacttc ctgacgaact gctggcgctg gtggacatta ttacgccaaa cgaaacggaa
     3721 gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag atgcagcgaa ggcggcgcag
     3781 gtactgcatg aaaagggtat ccgtactgta ctgattactt taggaagtcg tggtgtatgg
     3841 gctagcgtga atggtgaagg tcagcgcgtt cctggattcc gggtgcaggc tgtcgatacc
     3901 attgctgccg gagatacctt taacggcgcg ttaatcacgg cgttgctgga agaaaaacca
     3961 ttgccagagg cgattcggtt tgcccatgct gccgctgcga ttgccgtaac acgtaaaggc
     4021 gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat ttttagacag gcagaggtga
     4081 cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg tttctacctc aacagtttct
     4141 cacgttatca ataaagatcg cttcgtcagt gaagcgatta ccgccaaagt tgaagcggcg
     4201 attaaagaac tcaattacgc gccatcagct ctggcgcgta gcctcaaact caatcaaaca
     4261 cataccattg gcatgttgat cactgccagt accaatcctt tctattcaga actggtgcgt
     4321 ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg tcctttgcaa taccgaaggc
     4381 gatgaacagc ggatgaatcg caatctggaa acgctgatgc aaaaacgcgt tgatggctta
     4441 ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa tcatgcaacg ttatccgaca
     4501 gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg acagcgatct tattcaggat
     4561 aactcgttgc tgggcggaga cttagcaacg caatatctga tcgataaagg tcatacccgt
     4621 atcgcctgta ttaccggtcc gctggataaa actccggcgc gcctgcggtt ggaaggttat
     4681 cgggcggcga tgaaacgtgc gggtctcaac attcctgatg gctatgaagt cactggtgat
     4741 tttgaattta acggcgggtt tgacgccatg cgccaactgt tatcacatcc gctgcgtcct
     4801 caggccgtct ttaccggaaa tgacgctatg gctgttggcg tttaccaggc gttatatcag
     4861 gcagagttac aggttccgca ggatatcgcg gtgattggct atgacgatat cgaactggca
     4921 agctttatga cgccaccatt aaccactatc caccaaccga aagatgaact gggggagctg
     4981 gcgattgatg tactcatcca tcggataacc cagccgaccc ttcagcaaca acgattacaa
     5041 cttactccga ttctgatgga acgcggttcg gcatagattt acgctgtctt ttgatcaaat
     5101 tattaccatc ggttgttttc agaagcatga acattgctgc tgaagcaaca gtaataatgc
     5161 ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt tgtgccttcc atcccttcat
     5221 aaatgcgaag gacagccgca cttacagcaa cgcctaaact aatcgacagt tgctgcgtga
     5281 ccgccagaac attgttaccg ctgctggcgt tgtcatcggt cagatcggca agtgtgatgg
     5341 tattcatcgc ggtaaactgc gtcgacatag ccatccctaa tataaacaac ggcaagatca
     5401 gcatccatat cgccattgcc ggtgattgca aagagaactg agcgatcatt agcccaataa
     5461 tcaccgtgat ccccactaat gtatggcgat agcccagacg acgtaagact tgggtaacca
     5521 tcgattttgc aataatggaa cctaacgctg tcggtgccat catacagccg gcaataaacg
     5581 cctgataacc aaatcctacc tgtaacatca atggcataag gaacggtaca cagccggtcc
     5641 ccagacgggt tgcaatattg cctacgatac cgatcgagaa agtgcgggtt ttaaataaat
     5701 ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag aatatagaga agcagtaacc
     5761 cgatgctggt gacaattacc gtcaaggcaa tccagctggc gacaatcttt tccccgaata
     5821 gctctattcc gcttgaaaag agaacaaggc tgaggccaaa cagcaaaaag ccagtgatat
     5881 cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt gcgcgcgtaa agaaggcccg
     5941 caatacctat ggggatatta attaaaaata tccagtgcca ggttgcccag gtgaccagca
     6001 cgccgccaag aacggggcct aaaattggcc ccaccagacc cggcatggcg acaaaattca
     6061 ataccggaag aagttcatta cgaggataag cacgcagtaa ggccagccga gcaacaggca
     6121 tcatcattgc gtcgcctatc ccttgaataa cccggaagac aaccagctgt ggtagcgaat
     6181 tagaaagtgc gcaggccaga gaacccaacg tgaacagact cacggcaagg gtaaaaatgc
     6241 gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg aataagcatc gccaccgtca
     6301 gcgtataact gatgatggct gattgcatcg cgagaggaga acgattaagg ctatgagcga
     6361 ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc ctgcatgaaa aaggccatcg
     6421 ccgcgatcca cggcaaaccc gacatactac gcttcttttt atcgctcatt caatgtcctg
     6481 ttatcgggtt atcacttatc aggtgagcgt agcagcgcct gacaagcttt aaatgccgcg
     6541 tcgccatcgc tttggataat cgcatcgaca atcgcctggt gcagatccag ctttatcact
     6601 gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg aatggaatag cgaggcgaat
     6661 gaggtcaaaa acggattggc gctcatttca tagatatgct catgccaggc tatatcgact
     6721 tcgatccagc gttcacggcg aaagttctct tttaatgccg ccatttcggc cattaacgta
     6781 ttgagatgcg ccttctgttc cgcggtgcca accgttgctg ccagtaggca ggcttgcggc
     6841 tccagacaga tacgcataac cagaaagtga tcgatgacct gatgaaagtt ctcttctgtc
     6901 atccaccaag taagcaattc ctgatcaaga aaattccagt tcgattgtgg catgacccga
     6961 gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg ttaacgtttt gaccgcttcg
     7021 cgtaccgctg tacgactcac tccaaattgc tcgcccagct caatctcacc gggcaaaatg
     7081 gtgccgggtt catattcacc ttttaagatc cgttgcgcca gcttctcagc cagaacatac
     7141 gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag gcattactta tcttcctttt
     7201 tctttttatt cctccttagt atgccaccag gaagtgtgat tacggttgca aaaacggcaa
     7261 attgattgtt ttatggcaca ttaacggggc ttttgctgaa aaaatgcgcg gtcagaaaat
     7321 tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca ccactgacac
     7381 ggaacaacgg caaacacgcc gccgggtcag cggggttctc ctgagaattc cggcagagaa
     7441 agcaaaaata aatgcttgac tctgtagcgg gaaagcgtat tatgcacacc tcagcgccgc
     7501 tgagaaaaag cgaagcgcac tgctctttaa caatttatca ggcaatctgt gtgggcactc
     7561 gaagatacgg attcttaacg tcgcaagacg aaaagtgaat accaagtctc aagagtgaac
     7621 acgtaattca ttacgaagtt taattctttg agcatcaaac ttttaaattg aagagtttga
     7681 tcatggctca gattgaacgc tggcggcagg cctaacacat gcaagtcgaa cggtaacagg
     7741 aagcagcttg ctg
//
LOCUS       DAJTYG010000140         7732 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTYG010000140 DAJTYG010000000
VERSION     DAJTYG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.140"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..638
                     /locus_tag="OO568_003575"
                     /pseudo
     CDS             1..638
                     /locus_tag="OO568_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            635..1021
                     /locus_tag="OO568_003576"
     CDS             635..1021
                     /locus_tag="OO568_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642756.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGDYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LACWFHYKDGQWRVITYIFWPPGARF"
     gene            1046..3258
                     /gene="tssI"
                     /locus_tag="OO568_003577"
                     /pseudo
     CDS             1046..3258
                     /gene="tssI"
                     /locus_tag="OO568_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
     gene            3281..3725
                     /locus_tag="OO568_003578"
                     /pseudo
     CDS             3281..3725
                     /locus_tag="OO568_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3748..4155
                     /locus_tag="OO568_003579"
     CDS             3748..4155
                     /locus_tag="OO568_003579"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR6642757.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4152..6630
                     /locus_tag="OO568_003580"
                     /pseudo
     CDS             4152..6630
                     /locus_tag="OO568_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            7009..7365
                     /locus_tag="OO568_003581"
                     /pseudo
     CDS             7009..7365
                     /locus_tag="OO568_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010380972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
BASE COUNT         1968 a         1806 c         2259 g         1699 t
ORIGIN      
        1 atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat caaaatactg
       61 gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat accgttacag
      121 atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta tctgttcagc
      181 aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc gctaaaaaat
      241 ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag aatgaagact
      301 ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat gttgatttta
      361 aaaacagtac ggttcatgtt gattcagcgg acctggaaaa agtagcgaaa tgtattaact
      421 gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt ggttacttag
      481 atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg atgaatgtgg
      541 gaatggagct tcgggaacga taccttaaaa acgcaactat aacgttgaat gtcataatgc
      601 ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta ttatagtgat
      661 tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg cagttgctca
      721 atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca tcttttttat
      781 gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat ttgttggtga
      841 ttattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc aagatgttcc
      901 tattaaggaa atgttctgga atttgcaggg ggatttgaat ctggcatgtt ggtttcatta
      961 taaagacggg caatggagag tgattactta tattttttgg ccaccagggg ctagatttta
     1021 atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata aaccagttgg
     1081 aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg gtatgagcac
     1141 aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac aggcagcggc
     1201 ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag tagctgctgc
     1261 gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc aggatgcggc
     1321 ggcgtcgctt gcagccggtg gtccgggggc gcataacgtc acagtaagtg gtagtgcggt
     1381 gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg ggaatctgtt
     1441 ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg tttcccccgc
     1501 tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta tcgaactgga
     1561 cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg tcggccatga
     1621 gggccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc tgacccatac
     1681 cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg aagtactgga
     1741 tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc gcgcctggca
     1801 ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg aatgggggat
     1861 ttactggtgg tttgagcaca gcgagaacag ccacacgctg gtgctggtgg atgccatcaa
     1921 tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag ggctgaaact
     1981 ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg gaaagtgggt
     2041 gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg tggccagtcc
     2101 gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt acttcactac
     2161 gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc caggcagccg
     2221 gcacatgggg ccggtcatat ccgtaccctc atgaccgggt acacctttac gttgatgaat
     2281 cacccgacgg cagaaattaa tcaggaatat ctgctggtac agaccactct gtttttacgg
     2341 gataatgcgc agcacagcgg tcaggatcag cattttacct atgtcaccac gtttgaactg
     2401 ccccccacca gcgaagttta ccgcccacaa cgaaccctga gcaaacccca caccaaaggc
     2461 ccgcagagcg ccatcgtcac cggcccggcg gggcaggaaa tctggacgga taaatatggc
     2521 cgggtgaagg tgcagtttgg ctgggaccgt tacggaaaga atgacgaaaa cagttcctgc
     2581 tggatccgcg tcagctatcc gtgggcgggt aaaggattcg gcatgatcca gatcccgcgt
     2641 attggccagg aagtactggt ggatttcaaa aacggcgatc cggatttgcc catcatcgtg
     2701 gggcgcacct ataaccagga caccatgccg ccgtgggggc tgccgggcat ggcgtcgcag
     2761 agtgggatat tcagccactc attgcaggga gggccgacta acggaaatat gctgcgcttc
     2821 gatgataaaa cgggtgcgga agaggtgaag ttccatgcgg aaaaagacct caataccacg
     2881 gtaaaaaaca atgaaaccca cacggtcatg gtcgatcgca taaaaaccat tgtcaaaaat
     2941 gaaaccatca gcgtgggaga agaccgtaat gccaccatca caaaaaatga tggtctttcc
     3001 gtcaaactgg ctcaaaccat caatgtcgga acgacatatc gtctggatgt tggcgatcaa
     3061 tttacccttc gttgcggcaa tgcagcgctt gtattacaca aggatggctc gattgagttt
     3121 tgcggaaaac aactgttact gcacaccagc gatgttatgc aactcattgg cagtggcatt
     3181 gatatgaatc cggatggcgg cacagccatg actgcggatg atattgcccc gttacctacc
     3241 cccgacgttt ctgaataaaa aatataaaag gaatttttct atgaccagcc tttaccgtat
     3301 tcaggagggc tgctttgccc tgcctgaaac ttttcttgat cgcaccgtca atatcttcgt
     3361 cccatcgggc aatgaaaggg caactcccag tctgaatatt tcccgcgata cgctcaggcc
     3421 agatgaaagt ctgaccacct atattgaccg ccagatagcc ttgatgaaaa aaaaacctgg
     3481 gccagcaccg ggtactttcg cggggaacgg cactggcggg aactggcgaa gctgccatga
     3541 caggtgaaca gattctcgca actcatcgtt ctggaaaaac agaagtgtac cagcgtcagg
     3601 ccgggtttat caccgggcca gcaaaggtac taatgttgac gctgaccact cagcgtcctt
     3661 ttgatgatca caccgaccag ctatggactg catggttgac tagttttcag cctgctaaaa
     3721 gctaaaaaaa aacagggagg ttttgtcatg tatgaagccg cacgggtgga tgaccccatc
     3781 taccacacca gcgcccttgc cgggtttttg ataggggcca tcataggtat cgccattatt
     3841 gccgtcgcca gctttgcttt ttttacctgc ggctttttgg ccgggctgat tttgagtttt
     3901 ctggctgatc agatagcttc cggagtcctg caactgggtg aggctattgg gcgctcgata
     3961 cattccacag tgggaaaaat tctgaccggt tctgataatg tcagcaccaa cagtcgcccg
     4021 gccgcccggg ccgtactgag tacggtgata tgcgaagacc attccccgga aatccgcatt
     4081 gcccagggtt ccggcaacat ctacatcaac agccagctgc ccgtaaggat gaccacactg
     4141 agtgtgatgc agtgattgag gacggctccc ccaatgtctt tctcggcggc ggtacgcaga
     4201 cggtactgga aatcagcccg gaaatccctg actggctgcg tcaggtggtg gatgtgctgt
     4261 atgtggtcgc cggaatgctt ggcgggctgg ctggcgcctg gcgtcaggcg gcaaagatgg
     4321 ggtcgaaatt tggtacaaaa tgtgcggcaa aatttattgg tggcgaaatg gtcgggatgg
     4381 ccatctctga cacagtcatg ggattgttca gcaatccggt agatgtcacc actgggcaaa
     4441 aaattttgct gccggagacg gattttaccc tgccaggacg cctgcctgtc acctgttcgc
     4501 gcttttacgc cagccacctg gaaactgaag gattgctggg cagaggatgg cggctgaact
     4561 gggagataaa cctgcgtgag gatgaaacat atatcacctt catcggtgta cagggtcggg
     4621 aactgagtta tccgaaagag atgctgatac cgggccacca gatattcgac ccggaagagc
     4681 agttttacct gagccgtctg catgacggat gctatgtgct gcattacacc gaccgcagct
     4741 attacgtgtt tgatgaattt gatgaccacg gcgtcgcgcc gttacttttt atggagacgc
     4801 cgtaccggca gcgtattgct ttcgggcgtg agaacggcag gctggtgcgg gtggcctcca
     4861 gcagcggaca ccatctgtta ttgcaccgca cgatgacgca agcaggggaa cgcctgtcgc
     4921 atattgaact tgtgaagggc gggcgtcccg gtaacctggt ggaatatcgt tacgacgata
     4981 acgggcaact gactggcgtg gtgaaccggg ctggtgtgac ggtgcgccag tttgcctatg
     5041 aaaatggcct gatgacagag catcgcaacg cgacgggctt tacctgtacc taccactggg
     5101 aagagattga aggtttcccg cgcgtggtgg aacacacgac cagcgacggc gaggattacc
     5161 ggttccacta tgattttgct ggcgggcaga cggtggtaac cggcaggccg gagcagaagt
     5221 ggcagtggtg gtttgatgaa gagacgtatg tgactgcgca ccggacgccg ggcggcggaa
     5281 tgtaccgctt cacgtacaac gaaaaccatt ttccggtagc ggtggaactc cccggcgaac
     5341 gcagggtaac gctggaatac gacaccctga gccgggtggt gaaagagacg gacccggcag
     5401 gccgggtgac acagacgcag tggaacggca gttttgctga aatcacgcgc cgggcgctgg
     5461 atgacgacca cgtatggaag gcggattaca acgaacacgg ccaggtgata cgggagacgg
     5521 acccggaagg gcgcgtcacg cggtacgggt atgatgaaca ggggctggcg gtcagccgca
     5581 cggatgcgcg gggcggcgag gttgtgctgg cgcacgatgc gcgcggtcaa ctgaggcgtt
     5641 acaccgactg ttcagggtgc gccacgggtt atgaatacga tgaagacggc aacctgacgg
     5701 cggtgacgga cgcggagggg aaaacggtac gcatcagcta taaccgtcag gggctgccgg
     5761 agacggtctg ccaccccggt aaacagcagg accgttacac ctggaacgcg ctggggttac
     5821 tgagcagcca ccgtcgcatc acgggcagtg tgcagtcctg gcagtatacg ccgcgcggga
     5881 tgctggcgcg ccatacggat gaagagaaac gggagacgcg ctggcagtac accccggaag
     5941 ggctggttgc cagtctgagc aacggcaacg gggctcagta ccggttcagc tacgacggcg
     6001 acggacggct gacgggagaa cagcgcccgg acggcctgat aaggatgttt gcgctgaatg
     6061 cggacgggtt tccggtcatc atccagacgc agggcacaga gggcggggtg cgcaatgaac
     6121 agcaggagcg tgatgcgctg ggccgcctgc tgcgcagtga cacgcagcac agtacacgga
     6181 ccttcagcta caaccgtctt gaccagataa cggaagtgac gctcaccccc acggaagaag
     6241 gggaacggct gcaccacatg caggcggaca cggtgcgttt tgcgtatgac cgcagcgggt
     6301 ggctgacggc agagcacagc gtgcacggca gtatcaaata ccggcgcgat gcgctgggca
     6361 atcccaccga tatcacgttg ccggacgggc agcatctcag tcatctttac tacggcagcg
     6421 ggcatctgct ccagacggca ctggacggta tcacggtcag tgaatatgag cgtgacagcc
     6481 tgcaccgtca ggtgatacgc acgcagggga aactggcgac gttcagcggc tacaacgccg
     6541 acaaccggtt aagctggcag cgcagcctgc caggtggcag ccagaatcca cagcaggccg
     6601 gtactccccc ggcgcagaac gacagcgtga cggcgcggga ctatatctgg aacgcagacg
     6661 gtgaagtcgg cggcatcaat gacaaactgc gcggttgcct ggtgttcagc tatgaccgca
     6721 gcggctggct gaccagccgc acggggcaga tgtatgacca tgaccattat tattacgaca
     6781 aagcgggcaa cctgctgacg gatgagtatc agggagcggt gatggataac cgcctgccgg
     6841 gctacgggcg tgaccgttac cgctataacg aatggggcga actgacagag cgcagggacc
     6901 agcaactgga gtggaacgcg cagggccagt taacgcgggt catcagcagc aacagcgata
     6961 cgctgtatca gtatgatgcg ctgggcaggc gcatcagcaa ggtgacatat ggccgccatc
     7021 acggggcgcg gagagagcca gaaaatctgg tattaccaca cggacctgac gggcacggta
     7081 cacgaggtaa ccgcgccgga cgggacgctg gtgtgggcgg gctaccaggc gggatttggt
     7141 gaaaaccggg gcgacatcag caacagcggg gcgtactttg agcagccgca gcggctgccg
     7201 gggcagtact ttgacgaaga aacagggctg cattacaatc tgttcaggta ttatgcgccg
     7261 gagtgcggga ggttcgtaag ccaggacccg ataggattaa acggtgggtt gaacttatat
     7321 gcttatgcgc ctaatccgtt aggttggatc gatccgctag gactgaattc tttaggaata
     7381 gattcaaata atatatttag aggagatggt aactataaag gtggcggaat tggacagcca
     7441 aagagtggca caatatcagc acaggacatt attgatcatc tctaagaaaa aggtgatggg
     7501 cgtttaacat cttttagtac taaaaaaaca tctagttcca actctcgtgg tgcagatttt
     7561 ttctcaaaaa accatgtctt caaagtggca gtttctgatc tcgatgattt agttaaaaat
     7621 aatcagatca ctgttttaac acaagaaaaa gtaaaagcaa tatttggttt agaatcaagc
     7681 aagaaaatta gaaaagaact tggtaatatt cttcagaata tggctaggaa ta
//
LOCUS       DAJTYG010000141         7620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTYG010000141 DAJTYG010000000
VERSION     DAJTYG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.141"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            12..230
                     /locus_tag="OO568_003582"
     CDS             12..230
                     /locus_tag="OO568_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR6642758.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            448..690
                     /locus_tag="OO568_003583"
     CDS             448..690
                     /locus_tag="OO568_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642759.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1020..1949)
                     /gene="fdhE"
                     /locus_tag="OO568_003584"
     CDS             complement(1020..1949)
                     /gene="fdhE"
                     /locus_tag="OO568_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6642760.1"
                     /translation="MSIRIIPQDELGSSEKRTANMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1946..2581)
                     /gene="fdoI"
                     /locus_tag="OO568_003585"
     CDS             complement(1946..2581)
                     /gene="fdoI"
                     /locus_tag="OO568_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6642761.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2578..3480)
                     /gene="fdoH"
                     /locus_tag="OO568_003586"
     CDS             complement(2578..3480)
                     /gene="fdoH"
                     /locus_tag="OO568_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6642762.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3493..6543)
                     /gene="fdnG"
                     /locus_tag="OO568_003587"
     CDS             complement(3493..6543)
                     /gene="fdnG"
                     /locus_tag="OO568_003587"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5956..5958),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6642763.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLSDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6737..7570
                     /gene="fdhD"
                     /locus_tag="OO568_003588"
     CDS             6737..7570
                     /gene="fdhD"
                     /locus_tag="OO568_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6642764.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1740 a         1974 c         2051 g         1855 t
ORIGIN      
        1 aggtggtggt aatggcgatg aatacggttt ttcttcattt atcagaagag gcaattaaac
       61 ggctgaacaa gcttcggggc tggcgtaagg tttcacgatc tgcaatttta cgcgaagcgg
      121 ttgagcaata tctggagcgg cagcaatttc cggtgcgtaa agcaaaggga ggcaggcaaa
      181 agggggaggt agttggtgtt gatgatcagt gtaaggagca taaggaatga aaaaaatttt
      241 tcattcttga atataaaaaa cagatgccct tattctggta ttaatacaag gctgttttac
      301 ttgaacttat aataactgca actgttacat cgtatctgga aaacgcctcg caaaaataaa
      361 aaatgatgcg taaatgagct ttctttaaag attcggcaag ggtacttttc tctccataga
      421 ttcaaaaatg atattggcga gatatttatg aactctctgg caggtataga tatgggcaga
      481 attttactcg atttatcgaa tgaggtgatt aagcaacttg atgatctcga ggtgcagcgt
      541 aatcttcctc gtgcggacct attaagggaa gcggtagatc aatacctgat aaatcaatcg
      601 caaacagcaa gaaccagtgt tcctggcatc tggcaagggt gtgaggaaga tggtgtcgaa
      661 tatcagcgta agctgcgcga ggaatggtaa tgggaaaaaa tgatgtcaat cagattgctg
      721 acaacgtgcg cgttgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaagtc
      781 atgcaaattc aatatattga agtttattgg taggcctgat aagcgcagcg catcaggcaa
      841 tcatacgctt gtcatcagtg acatacccat aaccttatcg atgtgttgga gggaagaacg
      901 aggcaaacag acaattgcgg catgatcttg aatgggtaac gcggaatacg agagatggtg
      961 tcgtcacacc ctatgagctg taatccgggc gacctgcgcc acccggaaaa cgtcagagat
     1021 tactctcctt cacccggaaa caggaacggg ttaatggaac tacgggcgta gccttcttgc
     1081 tccattcgcg cgtccagtac cagagaggcg aggtcatctg ccacggcttc aactttcggt
     1141 tctttttcct gataaagaat cttcaggtaa gtgccgcagt catcgcagct ttcggcttta
     1201 atcgcggcct gttcgtcatc cagcgaccag taatgcagtt tgccgctctg ctcgcagttg
     1261 ctgcatttta cgcgcactac gtgccattcg gtttcacaca ggttgcagtg caggtaacgc
     1321 agaccctgag tggtgccaat ttgcaccatg ctggacaccg gcatagagcc acataccggg
     1381 caatattgac gttgttcgcc gtattcagcg cgggctttgc cggggatcag attggccatc
     1441 tgcgcccagt agagcgacag cgcagcccag ataaacggcg ctttatcgct gctgacggac
     1501 gagaaatcag aggcaaacag cgcgctggcc atatcttcca gctcctgagt cgatgccttc
     1561 tccagattct caatcactgc cagcgccggg ccgctcattt caggtttcag ctcagcaatt
     1621 agcgccatca gcagcttttg ccagtgctta tcacgcggca gaacgtgaat atccagcggg
     1681 ggcttgccct gtgcgctggc ttctttaatg cgcgcggtca gatccatctc cagcggatgg
     1741 tcgtacagca ccacttcctg ggcgtgggcg ataagcgcag caaagcgcag gtaatcaccc
     1801 agcggattat tttctgccag ctcgcgcaga cgctcggcgc ggcggttgta taaattcttg
     1861 agccgaggga acaataacgg cggaatcata ttcgccgtac gtttctcgct cgaacccagc
     1921 tcatcttgcg ggattatgcg aatactcatt cagctttctt ttccgttgtc ttgcggacct
     1981 cacggtacca gcgcgggtga tgtttcttcg cccaggcgct ggtaacccat ccttccacca
     2041 tggcggtaat cgtgcctttc acccaaaggg cggcgtagat atgcaccatg ataaccacaa
     2101 ttaacgccac tgcggcaaat gaatgcagca ttaacgcgaa tcggatcacc gggattgaga
     2161 aagcaggcgc aaaataagga cgccagataa tcacgccgct caccagcagc agaaccagga
     2221 aaataatcgc cgcccagaaa acgcatttct gaccgaagtt ataacgcccg gtgtcaccta
     2281 cttcctcgtt gacgacgatc ttacgaatat tcttcgccca aaagatatca tcccgattga
     2341 ttaggttttg atgccagtaa cggaaaaaca tgatgatgaa cgaggcaaac ataaccacgc
     2401 cgacaaacgg gtgcagaatt cgcgccagct gcggtgtgcc catgatttgc atcaaccagt
     2461 tgaaggacgg gaacaaaaag cccagcccgc tcaccgccgc caggatgaag cagaaggcgg
     2521 tgatccagtg gttgatacgt tccggcgcgg tgtagcgcac gatggtgtca cgtcgtttca
     2581 tttgcgctcc tcgtctttct cttcgtgcag attattctct tcctcatccg cacggttcgg
     2641 accgacaccc acgtagtgga agatactggc cgcaaaggta gccgcaaagc caacagccgc
     2701 gagcggtttc cagatgcctt tccagaattt cacggtttcg ctgatttccg ggttctccgg
     2761 caagccatga tacagatttg gcttgtcagc atggtgcagc acgtacatga cgtgtgtacc
     2821 accgacgcct gccggatcgt acagacccgc attgtcgtaa ccgcgggttt tcagctcagc
     2881 aacgcgctcg ctcgccagcg ttttcatcga ctctttcgta ccgaagtgaa tcgcgcccgt
     2941 tgggcaggtc ttcacgcagg ctggttcttg cccaaccacc acgcggtcaa cgcacagcgt
     3001 acatttgtag acgcggttgt cttccgggtt gaggcgcgga atgtcgaacg gacagcccgc
     3061 aatgcaataa ccgcagccaa tgcactgctc ggactggaag tcgacgatac cgttggcata
     3121 ctgaataatt gcaccttccg ccgggcacgc tttcaggcag cctggatcgg aacagtgcat
     3181 acagccgtct ttgcggatca gccattccag tttgtcgttc tgctccactt ccgagaagcg
     3241 cattaccgtc cacgatttgg cgcttaaatc attggggttg tcgtacaccc caatgttatt
     3301 gccgacggta tcgcgaatgt cgttccactc tgaacacgcc acctgacagg ctttacagcc
     3361 gatacaggtg gtaacgtcga tgagtttcgc cacttcttcc tggaagtccc gcgcctgagg
     3421 cgcgggggtc agaccgttag tcgcggaacg acgaatgata tcttgagatt gataagccat
     3481 atgtcgtctc cgttacacct tttccacatt cacaaggaag gacttaaact ccggcgtctg
     3541 cgtgttcgca tcaccaacga atggcgttaa agtattggca ataaagcctt ttttagctac
     3601 gccttcatag ccccagtgaa tcggaatacc gatggtatcg atatctttgc cgttcgcttt
     3661 cagcgtgcga atacgtttgg tcaccaccgc tttggctttg atatagccac ggttggagga
     3721 gactttcacg gtatcgcctt gggcaatgcc aagtttattc gccagcgact ccccgatttc
     3781 cacaaactgc tctggctgca aaatcgcgtt caacagcgcg tgtttggtcc agtagtggaa
     3841 gtgctcggtc agacgatagg tggttccgac atacgggaac ttatcggctt tacccaatgc
     3901 ttcggcgtcg tctttaaaga tacgtgcagc cgggttcgag ataacgtttg gatgcagcgg
     3961 gttagttccc agcggcgttt caaacggctc gtagtgttcc gggaacggac cttccgccat
     4021 cttatcgagg gcaaacagac gccccatgcc ttcctgctgc atgataaacg gcccgacgcc
     4081 gctacccgga ggcgctgcgc tgtagtccgg aatatcccag ccggtccact tagtgccgtc
     4141 ccatttcagc aactgacgct tcggatccca cgggttaccc tgcggatctg cggaggcgcg
     4201 gttatacaga atgcggcggt taagcggcca tgcccatgcc cagcccagcg tgttaccgag
     4261 gccagacgga tcggcgttat cacgacgcgc catctggttg ccttccggcg tccagctacc
     4321 ggcgaaaatc cagcagccac aggaggttgt accgtcatcg cgcaattggg cgaacgagct
     4381 aagttgttgg cctttcttga cgataaccgc accggttgcc ggatcggtaa tatcggccag
     4441 cgccttaccg ttgctctcca tcgctacttc ttccgacttc ggctcatgcg gaatggcgta
     4501 gttccaggtc atgttcagca cctgatccgg gttcgcgcca ccctgttcgg catacatctt
     4561 gcgcaagcgc aggaagatac cggagaggat ctcaccgtca gtcagcgcaa tccccggggc
     4621 gtccgcacct ttccagtgcc actgcaacca gcgacctgag ttaacgattg aaccgttctc
     4681 ttctgcgaag caggtcgatg gcagacggaa cacttcggtc tggatcttcg acgagtcaac
     4741 ttcgttcagc tcaccgtggt tctgccagaa gttagaggtt tcagtgttca gtgggtcgat
     4801 ggtgacgagg aacttcagtt tcgacagaca accgatcact ttgtttttgt tcgggaatga
     4861 ggcaacaggg ttaaagccct ggcagatata gccattgacc ttgccctctt tcatcatctc
     4921 gaagtattgc aggacgtcgt agcctttatc ccacttcggc aaccagtcaa agccccagct
     4981 attttccgcc gtcgctttat caccaaagaa ggccttcatc atagagacga agaatttcgg
     5041 gtagttgccc cagtagttta cctggccttc cagcagcggt tttggcgtgt tggcggtaag
     5101 gtaggtttgc agatcggtct gcttctcgct tggcagcgtc atgtaacctg gcagactctg
     5161 cgacagtagc cccaggtccg tcagcccctg aatattggag tgaccgcgca gggcgttaac
     5221 gccgccgccg gccatcccca tattgccgag cagcagctgg atcatcgcca tcgtacgaat
     5281 gttttgcgca ccaacggagt gttgcgtcca gccgagggca tacaggaacg aggcagtttt
     5341 atcgtgagca ctggtttctg cgatgtattc gcagactttc aggaacgcgt cttttggcgt
     5401 accacagatg ttttcaacca catctggcgt gtagcgggaa acgtgctgtt tcagcaagtt
     5461 ccacacgcag cgcggatgtt gcagcgtggt atcgcgtttg gcgaagccgt tttcgtccag
     5521 ttcataagtc caggaggatt tatcgtactt gcgtttttcc gcgtcgtagc cggtgaacag
     5581 gccatcttca aagccgtaat cctcacgcac gatcaggctg gcgttggtat aggcttcggt
     5641 gtattcgtgg ttgaattttt cattgttcag caggtacagc aatacgcctg acaggaaagc
     5701 aatgtcagta ccggaacgaa taggggcata gtagtcagct accgccgccg tacgcgtaaa
     5761 gcgaggatcg atcacaatca gcttcgcgcc gttgtgaatt ttggcttcca tcgcccagcg
     5821 gaacccgacc gggtgagctt cagcggcgtt accgcccatc accacgacga ggttggcgtt
     5881 cttgatgtcg acccagtggt tggtcatcgc accgcgacca aatgttggag caagacttgc
     5941 taccgttggt ccgtgtcaga cacgcgcctg gttgtcgacc gcgagcatac ccagcgcgcg
     6001 ggagaatttt tgcgttaaat agccggtttc gttactcgac gcggaagcac acagcatccc
     6061 ggtggagagc cagcggttaa cagtcacgcc ttcggcgttt tgcgcaatgt agttagcatc
     6121 gcggtcttct ttcatcagtt tggcgatgcg atcaaacgcc tcttcccaac tgatttgctg
     6181 ccatttatca gaacctggcg cacggtattc cggaaacttc agacggcttt cggagtggat
     6241 gaaatccacc aggccagcgc ctttcggaca aagtgcaccg cggttgaccg ggtgatccgg
     6301 atcgccttcg atatggaaga tagatgcttt ggcgtttttt gctccgtcac tgaggctgta
     6361 catcaacagc ccacagccta cggaacaata ggtgcaggta ttacgggttt cgcgggtgcg
     6421 cagcagttta tactgccggg tttccgcgag tgctacgctg ggtgcaaaac ccagtgccgc
     6481 tgccgtggtg cctgccatac cgccagcgca gatcttaaag aactgccttc tgctgacctg
     6541 catggattgc tccttgtttc gacattgtca cgtcccattt acattcgctt gctgcgtgtg
     6601 cagggagtgg gagttatttt tctttgcgga aggggccgca aaggtccaga attggctcaa
     6661 tttccctcca tctaggaagg gtttgtaaca gaataccata atgttggtgt gtgtgttctt
     6721 atctggttaa gagaaagtga aaaaaacaca gcgaaaagaa atcgaaaatg tgacaaatat
     6781 cacaggtgtt cgtcaaattg agttatggcg gcgtgatgat ttacaacacc cacggttaga
     6841 tgaggtcgcg gaagaagttc ccgttgcgct ggtctacaac ggcatttcgc atgtggtgat
     6901 gatggcgtcg cccaaagacc ttgagtactt tgcgctcggt ttttcgcttt ccgaggggat
     6961 aatagaaagt ccgcgcgata tcttcggcat ggatgtcgtt ccttcctgta atggtcttga
     7021 agtacaaatt gagctttcca gccgccgctt tatggggttg aaggagcgcc gccgggcgct
     7081 ggcgggacgt acgggctgtg gcgtatgcgg cgtggagcaa cttaatgaca tcggaaaacc
     7141 ggtgcagccg ctaccgttca cccagacgtt tgatctcaac aaactggatg atgcattacg
     7201 tcatctcaac gattttcagc cagtggggca actgactggt tgtactcacg ccgctgcctg
     7261 gatgttgcca tctggcgaac tggtcggcgg gcatgaagac gtgggtcgcc atgtggcgct
     7321 ggacaaactg ttaggccgcc ggtcacaaga aggggaaagc tggcagcaag gtgcggtact
     7381 ggtttccagt cgtgccagtt atgaaatggt gcaaaagtcg gcgatgtgcg gcgtagagat
     7441 tttgtttgcg gtgtctgccg cgaccacgct tgctgtagaa gtggctgagc gctgtaatct
     7501 gacactggta ggtttttgta aaccgggtcg ggcaacggtt tatacccatc cgcagcgttt
     7561 gagcaattaa ttaaaataag tcctgcgata tatattaaat tgcaggactt attcatttcg
//
LOCUS       DAJTYG010000142         7507 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTYG010000142 DAJTYG010000000
VERSION     DAJTYG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.142"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            119..1230
                     /gene="yneK"
                     /locus_tag="OO568_003589"
                     /pseudo
     CDS             119..1230
                     /gene="yneK"
                     /locus_tag="OO568_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            1380..2570
                     /gene="ydeA"
                     /locus_tag="OO568_003590"
     CDS             1380..2570
                     /gene="ydeA"
                     /locus_tag="OO568_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6642765.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2595..3260)
                     /gene="marC"
                     /locus_tag="OO568_003591"
     CDS             complement(2595..3260)
                     /gene="marC"
                     /locus_tag="OO568_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6642766.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3472..3906
                     /gene="marR"
                     /locus_tag="OO568_003592"
     CDS             3472..3906
                     /gene="marR"
                     /locus_tag="OO568_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6642767.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3926..4309
                     /gene="marA"
                     /locus_tag="OO568_003593"
     CDS             3926..4309
                     /gene="marA"
                     /locus_tag="OO568_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR6642768.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            4341..4559
                     /gene="marB"
                     /locus_tag="OO568_003594"
     CDS             4341..4559
                     /gene="marB"
                     /locus_tag="OO568_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6642769.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4590..5489)
                     /gene="eamA"
                     /locus_tag="OO568_003595"
     CDS             complement(4590..5489)
                     /gene="eamA"
                     /locus_tag="OO568_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR6642770.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5753..6814
                     /gene="ydeE"
                     /locus_tag="OO568_003596"
     CDS             5753..6814
                     /gene="ydeE"
                     /locus_tag="OO568_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR6642771.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAA"
     gene            6998..7093
                     /gene="mgtS"
                     /locus_tag="OO568_003597"
     CDS             6998..7093
                     /gene="mgtS"
                     /locus_tag="OO568_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR6642772.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(7342..>7507)
                     /locus_tag="OO568_003598"
     CDS             complement(7342..>7507)
                     /locus_tag="OO568_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6642773.1"
                     /translation="KNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAA
                     FREKYHQMAA"
BASE COUNT         1957 a         1823 c         1706 g         2021 t
ORIGIN      
        1 gtccgttaaa ctcaggctac ctcaaacagc gccacgcatc acctgccttt gatagttctt
       61 gcagctcaaa tgccctatat agtgatgttc cgatgactta tgactatatg gggcaaatat
      121 ggttacgcca gtaagcatca gcaattacat atctcttcct gatgattttc ccgctcgtaa
      181 tattgcacct caagtaaaag aagttttaaa ggattttatt gatgcactca gtaccataat
      241 atgtgatgaa gagtggcgca cgtctttaaa caccaactca gccacaaaaa agatatttaa
      301 caaccttgat aacttatcat atattcagag gacttctttt cggggtaacg acacactata
      361 caatgaaaag gttcagttta aactcactta tcccgtcaag aatggaagac acaaagaaaa
      421 tattgaattt caggtagtaa taaatttaag ccctatttag ataattttcg ccatgatgga
      481 gaaattaata ttttttgcgc tcccaaccca aagcctgtca ctatggggcg cgtatttcag
      541 accggcgtcg agcgtgtgct ctttctgttt atgaatgatt ttattgaaca atttccaatg
      601 atcaaccttg gcgcccccat caaaagagcg catacaccac atattgaacc cctgcctccg
      661 gatcaccata ccgctgcaga ttacttacgc cagtttgatt tgcttgtcct gaattttatc
      721 tctcgcggta attttgtcat actcccccga ttatggaata actctgaggt tcacagatgg
      781 ttcgtcaata aagatcctaa cttgatcacc gccattctcg acataacgga cagcgaatta
      841 aaagaggatt tattgcaaag cctgatggat tcattaggtt ctaacaaaca tgtacaaccc
      901 gaagtctgca tctgcttctt atccctttta gcagaacaag aatctcctca ttttcaggac
      961 ttgtttttat ttttcgccaa tatgttattg cactatcacc aatttatgaa tcccaatgaa
     1021 agtgatctga atgacgtgtt gatgccagca tcattaagtg atgataaaat tatcaaacat
     1081 atggcgcgca ggaccctcaa actgtttgta aaaaatgaaa cgccgccaaa agttactcac
     1141 gaagacctgg tgaaaaacag gcctcgctcc cctgtcagac cacctatacc cgcaaccgcc
     1201 aaaacgccag acctccctga acgtcattaa accgtgatgt taccgactct ctgacgcgga
     1261 acagaatcag cgtcagagaa acggaaaacg cgatccagat cacaaatgca ttgtattcac
     1321 atcattaacc gttttaagat catttcatca ctttttcgca actcacccga taatctgtta
     1381 tgacaacaaa cactgtttcc cgcaaagtgg cgtggctacg ggtcgttacg ctggcagtcg
     1441 ccgccttcat cttcaacacc accgaatttg tccctgttgg cctgctctct gacattgcgc
     1501 aaagttttca catgcaaacc gctcaggtcg gcatcatgtt gaccatttac gcatgggtag
     1561 tagcgctaat gtcattgcct tttatgttaa tgaccagtca ggttgaacgg cgcaaattac
     1621 tgatctgcct gtttgtggtg tttattgcca gccacgtact gtcgttttta tcgtggagct
     1681 ttaccgttct ggtgatcagt cgcattggtg tggcttttgc acatgcgatt ttctggtcga
     1741 ttacggcgtc tctggcgatc cgtatggctc cggccgggaa gcgggcacag gccttgagtt
     1801 taattgccac cggtacagca ctggcgatgg tcttaggttt accgctcggg cgcattgtgg
     1861 ggcagtattt cggctggcga atgaccttct tcgcgattgg tattggggcg cttgttaccc
     1921 ttttgtgcct gattaagtta cttcccttac tgcctagtga gcattccggt tcattgaaaa
     1981 gcctcccgct attgttccgc cgcccggcat tgatgagcat ttatttgtta actgtggtgg
     2041 ttgtcaccgc ccattacacg gcatacagct atatcgagcc ttttgtgcaa aacattgcgg
     2101 gattcagcgc caactttgcc acggcattac tgttattact cggtggtgcg ggcattattg
     2161 gcagcgtgat tttcggtaaa ctgggtaatc aatatgcgtc tgcgttggta agtacggcga
     2221 ttgcgctgtt gctggtgtgt ctggcactgc tgctacctgc ggcgaacagt gaaatacacc
     2281 tcggggtgct gagtattttc tgggggatcg cgatgatgat catcgggctt ggtatgcagg
     2341 ttaaagtgct ggcgctggca ccagatgcta ccgacgtcgc gatggcgcta ttctccggca
     2401 tatttaatat tggaatcggg gcgggtgcgt tggtaggtaa tcaggtgagt ttgcactggt
     2461 caatgtcgat gattggttat gtgggcgcgg tgcctgcttt tgccgcgtta atatggtcaa
     2521 tcattatatt tcgccgctgg ccagtgacac tcgaagaaca gacgcaatag ttgaaaggcc
     2581 cattcgggcc ttttttaatg gtacgtttta atgatttcca ggatgccgtt aataataaac
     2641 tgcacaccca tacataccag caggaacccc atcaggcggg agatcgcttc aatgccaccc
     2701 ttgcccacca gccgcataat tgcgccggag ctgcgtaggc ttccccacaa aataaccgcc
     2761 accaggaaaa agatcagcgg cggcgcaacc atcagtaccc aatcagcgaa ggttgaactc
     2821 tgacgcactg tggacgccga gctaataatc atcgctatgg ttcccggacc ggcggtactt
     2881 ggcattgcca gtggtacaaa ggcaatattg gcactgggtt catcttccag ctcttccgac
     2941 ttgcttttcg cctccggtga atcaatcgct ttctgttgcg gaaaaagcat ccgaaaaccg
     3001 ataaacgcga cgattaagcc gcctgcaatt cgcagaccgg gaatcgaaat gccaaatgta
     3061 tccatcacca gttgcccagc gtaatacgcc accatcatga tggcaaatac gtacaccgag
     3121 gccatcaacg actgacgatt acgttcggca ctgttcatgt tgcctgccag gccaagaaat
     3181 agcgcgactg ttgttaatgg gttagctaac ggcagcaaca ccaccagccc caggccaatt
     3241 gctttaaaca aatctaacat tggtggttgt tatcctgtgt atctgggtta tcagcgaaaa
     3301 gtataagggg taaacaagga taaagtgtca ctctttagct aaccttgcat cgcattgacc
     3361 aaaacttgaa ccgatttagc aaaacgtggc atcggtcaat tcattcattt gacttatact
     3421 tgcctaggca atattatccc ctgcaactaa ttacttgcca gggcaactaa tgtgaaaagt
     3481 accagcgatc tgttcaatga aattattcca ttgggtcgct taatccatat ggttaatcag
     3541 aagaaagatc gtctgcttaa cgagtatctg tctccgctgg atattaccgc ggcacagttt
     3601 aaggtgctct gctctatccg ctgcgcggcg tgtattactc cggttgaact gaaaaaagtg
     3661 ttgtcggtcg acctgggagc actgacccgt atgctggatc gcctggtctg taaaggctgg
     3721 gttgaaaggt tgccgaaccc gaatgataag cgcggcgtac tggtaaaact taccaccagc
     3781 ggcgcggcaa tatgcgaaca atgccatcaa ttagttggcc aggacctgca tcaagaatta
     3841 acaaaaaacc tgacggcgga cgaagttgca acacttgagc atttgcttaa gaaagtcctg
     3901 ccgtaaacaa aaaagaggta tgacgatgtc cagacgcaat actgacgcta ttaccattca
     3961 tagcattttg gactggatcg aggacaacct ggaatcgcca ctgtcactgg agaaagtgtc
     4021 agagcgttcg ggttactcca aatggcacct gcaacggatg tttaaaaaag aaaccggtca
     4081 ttcattaggc caatacatcc gcagccgtaa gatgacggaa atcgcgcaaa agctgaagga
     4141 aagtaacgag ccgatactct atctggcaga acgatatggc ttcgagtcgc aacaaactct
     4201 gacccgaacc ttcaaaaatt actttgatgt tccgccgcat aaataccgga tgaccaatat
     4261 gcagggcgaa tcgcgctttt tacatccatt aaatcattac aacagctagt tgataacgtg
     4321 acaacgtcac tgaggcaatc atgaaaccac tttcatccgc aatagcagct gcgcttattc
     4381 tcttttccgc gcagggcgtt gcggaacaaa ccacgcagcc agttgttact tcttgtgcca
     4441 atgtcgtggt tgttccccca tcgcaggaac aaccaccgtt tgatttaaat cacatgggta
     4501 ctggcagtga taagtcggat gcgctcggcg tgccctatta taatcaacac gctatgtagt
     4561 ttgttctggc cccgacatct cggggcttat taacttccca cctttaccgc tttacgccac
     4621 cgcaagccaa atacattgat atacagcccg gtcataatga gcaccgcacc taaaaattgc
     4681 agacccgtta agcgttcatc caacaatagt gccgcacttg ccagtccgac tacgggcacc
     4741 agtaacgata acggtgcaac ccgccaggtt tcatagcgtc ccagtaacgt cccccagatc
     4801 ccataaccaa caattgtcgc cacaaacgcc agatacatca gcgacaagat ggtggtcata
     4861 tcaatagtaa ccagactgtg aatcatggat gcggaaccat cgagaatcag cgaggcaaca
     4921 aagaagggaa tgattgggat taaagcgctc cagattacca gcgacatcac cgccggacgc
     4981 gttgagtgcg acatgatctt tttattgaag atgttgccac acgcccaact aaatgctgca
     5041 gccagggtca acataaagcc gagcatcgcc acatgctgac cgttcagact atcttcgatt
     5101 aacaccagta cgccaaaaat cgctaaggcg atccccgcca attgtttgcc atgcagtcgc
     5161 tccccgaaag taaacgcgcc aagcacgata gtaaaaaacg cctgtgcctg taacaccagc
     5221 gaagccagtc cagcaggcat accaaagtta atggcgcaga acagaaaggc aaactgcgca
     5281 aaactgatgg ttaatccata ccccaagagc aaattcagtg gtactttcgg tcgtgcaaca
     5341 aaaaagatgg ccggaaaagc gaccagcata aagcgcaaac cggccagcat cagcggtggc
     5401 atgttatgaa gccccacttt gatgaccaca aaatttagcc cccatacgac cactaccagt
     5461 agcgccaaca ccccatcttt tcgcgacatt ctaccgcctc tgaatttcat cttttgtaag
     5521 caatcaactt agctgaattt acttttcttt aacagttgat tcgttagtcg ccggttacga
     5581 cggcattaat gcgcaaataa gtcgctatac ttcggatttt tgccatgcgt ccgatggtta
     5641 ataacaacga cgaggcaaga agggcgctgg tagagcgtcg tagggataag ttcatttgtc
     5701 tgtccggcag tgcgtttcgt tatgttttat tttagagatg taaagaaata gcatggctac
     5761 gctgccattt atgaccattt acttgagtcg ccagtacagc ctgagtgtcg atctaatcgg
     5821 ttatgcgatg acaattgcgc tcactattgg cgtcgttttt agcctcggtt ttggtatcct
     5881 ggcggataag ttcgacaaga aacgctatat gttactggca attaccgcct tcgccagcgg
     5941 ttttattgcc attcctttag tgaataacgt gacgctggtt gtgctctttt ttgccctcat
     6001 taactgcgcc tattctgttt ttgctaccgt gctgaaagcc tggtttgccg acaatctttc
     6061 gtccaccagc aaaacgaaaa tcttctcaat caactacacc atgctaaaca ttggctggac
     6121 catcggtccg ccgctcggca cgctgttggt gatgcagagc atcaatctgc ccttttggct
     6181 ggcagctatc tgttccgcgt ttcccatgct tttcattcaa atatgggtaa agcgcagcga
     6241 gaaaatcatc gccacggaaa caggcagtgt ctggtcgccg aaagttttat tacaagataa
     6301 agcactgttg tggtttacct gctctggttt tctggcttct tttgtaagcg gcgcatttgc
     6361 ttcatgcatt tcacaatatg tgatggtgat tgctgatggg gattttgccg aaaaggtggt
     6421 cgcggttgtt cttccggtga atgctgccat ggtggttacg ttgcaatatt ccgtgggccg
     6481 ccgacttaac ccagctaaca tccgcgcgct gatgacagca ggcaccctct gtttcgtcat
     6541 cggactggtc ggttttattt tttccggcaa caacctgcta ttgtggggta tgtcagctgc
     6601 ggtatttact gtcggtgaaa tcatttatgc gccgggcgag tatatgttga ttgaccatat
     6661 tgcgccgcca ggaatgaaag ccagctattt ttccgcccag tctttaggct ggcttggtgc
     6721 cgcgattaac ccattagtga gtggcgtagt gctaaccagc ctgccgcctt cctcgctgtt
     6781 tgtcatctta gcgttggtga tcattgctgc gtaggtgctg atgttaaaag ggattcgtgc
     6841 aagaccgtgg gggcagcccg cgctttgttg atttaagtcg aacacaataa aaatttaatt
     6901 cagccttcgt ttaggttatc tctgctaata tctttctcat tgagatgaaa attaaggtaa
     6961 gcgaggaaac acaccacacc ataaacggag gcaaataatg ctgggtaata tgaatgtttt
     7021 tatggccgta ctgggaataa ttttattttc tggttttctg gccgcgtatt tcagccacaa
     7081 atgggatgac taatgaacgg agataatccc tcacctaacc ggccccttgt tacagttgtg
     7141 tacaaggggc ctgattttta tgacggcgaa aaaaaaccgc cagtaaaccg gcggtgaatg
     7201 cttgcatgga tagatttgtg ttttgctttt acgctaacag gcattttcct gcactgataa
     7261 cggatcgttg acacagtagc atcagttttc tcaatgaatg aggtgtactg gcaatagcgg
     7321 acactaccat ttgttctttt tttaagcagc catctgatga tatttttccc tgaaggctgc
     7381 cggggagata ttccccagac gagagtgacg acgctgacga ttgtagaaaa tctcaatgta
     7441 ttcccgtatt actgagatgg cttcatcccg gttattaaaa cgatagtggc tcaggctctc
     7501 atttttc
//
LOCUS       DAJTYG010000143         7492 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTYG010000143 DAJTYG010000000
VERSION     DAJTYG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.143"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..1677
                     /gene="cpdB"
                     /locus_tag="OO568_003599"
     CDS             <1..1677
                     /gene="cpdB"
                     /locus_tag="OO568_003599"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6642774.1"
                     /translation="KGLKAGDVHPVYKALNTLDYTVGTLGNHEFNYGLDYLKNALAGA
                     KFPYVNANVIDARTKQPMFTPYLIKDTEVVDKDGKKQTLKIGYIGVVPPQIMGWDKAN
                     LSGKVTMNDITETVRKYVPEMREKGADVIVVLAHSGLSADPYKVMAENSVYYLSEIPG
                     VNAIMFGHAHAVFPGKDFADIEGADITKGTLNGVPAVMPGMWGDHLGVVDLQLSNDSG
                     KWQVTQAKAEARPIYDIANKKSLAAEDSKLVETLKADHDATRQFVSKPIGKSADNMYS
                     YLALVQDDPTVQVVNNAQKAYVEHYIQGDPDLAKLPVLSAAAPFKVGGRKNDPASYVE
                     VEKGQLTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIAPNSTKPQSLINW
                     DGFRTYNFDVIDGVNYQIDVTQPARYDGECQMINVNAERIKNLTFNGKPIDPNAMFLV
                     ATNNYRAYGGKFAGTGDSHIAFASPDENRSVLAAWIADESKRAGEIHPAADNNWRLAP
                     IAADKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(1803..2183)
                     /locus_tag="OO568_003600"
     CDS             complement(1803..2183)
                     /locus_tag="OO568_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6642775.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            2272..3132
                     /gene="qorB"
                     /locus_tag="OO568_003601"
     CDS             2272..3132
                     /gene="qorB"
                     /locus_tag="OO568_003601"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6642776.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAALVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIARPTTTLAE
                     SVSHLFNVNN"
     gene            3241..4206
                     /locus_tag="OO568_003602"
     CDS             3241..4206
                     /locus_tag="OO568_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6642777.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLSQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            4314..4976
                     /gene="ytfE"
                     /locus_tag="OO568_003603"
     CDS             4314..4976
                     /gene="ytfE"
                     /locus_tag="OO568_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6642778.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(5021..6433)
                     /gene="cycA"
                     /locus_tag="OO568_003604"
                     /gene_synonym="dagA"
     CDS             complement(5021..6433)
                     /gene="cycA"
                     /locus_tag="OO568_003604"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6642779.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDFSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(6741..7361)
                     /gene="fklB"
                     /locus_tag="OO568_003605"
     CDS             complement(6741..7361)
                     /gene="fklB"
                     /locus_tag="OO568_003605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6642780.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         1927 a         1965 c         1907 g         1693 t
ORIGIN      
        1 aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac gctggattat
       61 acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct gaaaaatgcg
      121 ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag aaccaaacag
      181 ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga cggaaaaaaa
      241 cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg ctgggataaa
      301 gctaatttat ccggaaaagt gacgatgaat gatattaccg aaaccgtgcg caaatacgtg
      361 cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc tggcgcattc cgggctgtct
      421 gccgatccgt ataaagtaat ggcggaaaac tcagtttatt acctcagtga aattccgggc
      481 gttaacgcca ttatgtttgg ccatgctcac gccgttttcc cgggtaaaga ttttgctgat
      541 atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt aatgccgggc
      601 atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag cggtaaatgg
      661 caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg acatcgccaa taaaaaatcc
      721 ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga tgccacacgc
      781 cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta tctggcgctg
      841 gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta tgtcgaacat
      901 tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc cgcaccgttt
      961 aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa aggccagttg
     1021 accttccgta atgccgccga tctttatctc taccccaata cgctgattgt agtgaaagcc
     1081 agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa ccagattgct
     1141 cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac ttataacttt
     1201 gatgttattg atggtgtgaa ttatcagatt gatgttaccc aacccgcccg ctatgacggc
     1261 gagtgccaga tgattaatgt caatgcagag cggattaaga acctgacctt taatggcaag
     1321 ccgattgatc cgaacgctat gttcctggtt gccaccaata actatcgcgc ttacggcggc
     1381 aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga gaaccgctcg
     1441 gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca tccggcggca
     1501 gataacaact ggcgtttagc accgatagct gccgataaga aactggatat ccgtttcgaa
     1561 acttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta tccgatgaat
     1621 aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag taagtaaaac
     1681 acttcttttt cggcctataa atcaaccgca tccggcattt attggcggat gcgatgctgc
     1741 cgtatcttat ccaccctaca agccatgaac cgtagaccgg ataagcgcag cgcatccggc
     1801 agttatgccg cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa ctcaatccag
     1861 tccgccagtg cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag gctatattcc
     1921 acatgcggcg gcaccaccgg atacgcgata cggttaagaa aaccatcctg ttctaacgcc
     1981 tgtaacgact gcgcaagcat cttttcactc accccaccga ttttgcgccg caggtcgcta
     2041 aagcgatgag taccttcgcg tagcgccacc agaatcaaca ccccccaacg gctggtgacg
     2101 tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga gattaccctc tttcagttgt
     2161 tgtgacaggc taacctgact catttcatac ttaccttttt gcatgtactt actaaaagta
     2221 agtttaggtg ttagcgtatt taaacacaag acaaaacgat ggagacttcc catgatcgct
     2281 attactggtg ccactggcca acttggtcac tatattattg aatccttgat gaaaacggtt
     2341 cctgccagcc aaatagtggc tatcgttcgt aatccggcaa aagcccaggc cctggcagca
     2401 caaggcatta ccgtgcgtca ggctgactac agcgatgaag ccgcactgac atctgcactt
     2461 cagggagtgg aaaaactact gctgatctct tccagcgaag tgggtcaacg tgccccgcag
     2521 catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc ttataccagc
     2581 ctgctacatg cagatacctc cccgctcggc ctcgccgatg agcacatcga gacggagaaa
     2641 atgctggctg attctggcat cgtttacacc ctgctgcgca acggctggta cagcgaaaac
     2701 tacctcgcca gcgccccggc agcactggaa cacggcgtat ttatcggtgc ggcgggcgat
     2761 ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag ctgcggcact cgtgattagc
     2821 gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg gacgttgaca
     2881 cagttagcgg cagagctgac caaacagagt ggcaaacagg ttacctatca aaatctgagc
     2941 gaagccgatt tcgccgcggc actgaaaagc gtcggactgc ccgacggact ggcggatatg
     3001 ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag caaaacgctt
     3061 agcaaattga ttgcccgccc aacgacaacg ttagctgaaa gcgtaagcca tctttttaat
     3121 gttaataact agttaattaa agtggcatcc tcccgcatcc tctctgataa tgacgggatg
     3181 ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga cagaccaatg gatgccagta
     3241 atgattagcg gcgtgctgta cgccctgtta gcagggttga tgtgggggct tatttttgtc
     3301 gggccgttga tcgtgccgga atacccggcg atgttacagt cgatggggcg ttatctggcg
     3361 ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg tcagttggcg
     3421 cgtcgggact ggcttaccgc cttgatgctc actatgatgg gcaacctcat ttattacttc
     3481 tgccttgcca gtgccattca acgtactggc gcgcctgttt ccacgatgat tatcggcacc
     3541 ctgccggtgg tgattcctgt ctttgccaat ctgctttata gccagcgcga cggcaaactc
     3601 gcgtggggaa aactcgcccc ggcactgatt tgtattggca tcggcctggc gagtgtgaat
     3661 attgctgagt taaaccacgg actccccgat tttgactggg cacgttatac ctctggcatc
     3721 gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa cgcccgctgg
     3781 ctgcgggaaa atcccgacaa acatccgatg atgtgggcga cggcgcaggc gctggtcacg
     3841 ctgccggttt ctctcatcgg ctatctcgtc gcctgttact ggctgaatat acaaacgccg
     3901 gacttctcct taccttttgg cccccgtccg ctggtgttta ttagtctgat ggttgcgata
     3961 gccgtgcttt gctcatgggt tggcgcactc tgctggaacg tcgccagcca gcgattaccg
     4021 acagtgattc tcgggccgct gattgttttc gaaacgctgg caggtttgct gtacaccttt
     4081 ttactcagcc agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct gttagtgatt
     4141 ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt taactgaatc tgtctcagaa
     4201 agttgacatg ctggcagtga gttaaataag cctctgctac gtaagggtta tagcttttgc
     4261 cttaaaggtg tatttaaaat acatcttatc ttattaagaa tgaggtatca gctatggctt
     4321 atcgcgacca acctttaggt gaactggcgc tctctatccc tcgcgcttcg gctctgtttc
     4381 gtaaatatga tatggattac tgctgtggcg gtaagcagac gctggcgcgc gcggcggcac
     4441 gtaaagaact ggatgttgag gtcattgaag ctgaactggc aaagcttgct gaacaaccga
     4501 ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat tgaccatatc atcgtgcgct
     4561 accacgatcg tcaccgcgag cagctgccgg agctgatcct gcaagcgact aaagtcgagc
     4621 gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac aaaatacctg accatgctgc
     4681 atgaagagct ttccagccac atgatgaaag aagagcagat cctcttcccg atgatcaaac
     4741 aaggcatggg cagccaggca atggggccaa tcagcgtaat ggaaagcgag cacgatgaag
     4801 cgggcgaact gctggaagtg atcaaacaca ctaccaataa cgtcacaccg ccgccagaag
     4861 cctgcaccac ctggaaagcg atgtataacg gcattaatga actgattgat gacctgatgg
     4921 atcacatcag tctggaaaac aatgtactgt tcccacgcgc cctagcgggt gagtgatcta
     4981 aaagctggat ggcattgcgc catccagcat gataatgcgg ttatttccgc agttcagcag
     5041 cccgcttctt accaataaac agccagccca accccagcgc gataaaccac agcggggtaa
     5101 ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg aacacaaaga
     5161 acgccataca tacccagcac atcagcttgc cgagcggcat cttgtagatc gacttttcat
     5221 gcagatgagg acgctgtttg cggtacacca ggtacgagca aaggataatc gtccagacga
     5281 acataaacag aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc acgctaggat
     5341 tcacatacaa catcaccacg ccgccgagca gacagataca cgagaaggtc aaccctttcg
     5401 cgggtactgc gcgcttagaa agtttagcga acgctttcgg cgcaacacct tcctgcgcca
     5461 gaccaaacag catacggctg gtagagaaga caccgctgtt agcggaagac gccgcagagg
     5521 tcagcaccac aaagttgatc acgctggcag ccgcaggcaa acctaccaac acaaacagct
     5581 caacgaacgg gcttttctcc gggactaccg aactccacgg tgtcacggac ataatcacaa
     5641 tcagcgcgaa gacgtagaac ataatgatac ggatcggaat ggagttaatc gcgcgtggca
     5701 gtgatttctc cggatctttg gtttccgcag ctgttgtacc taccagctca atccccacga
     5761 aagcgaaaac cgctatctgg aatccggcaa agaagccact taaacctttc gggaaccagc
     5821 cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc ggtcggtgac tgaaagtgca
     5881 tcgccaccat gaccaggccg acgacaatca gcgacacgat ggcgacgatt ttgatcatcg
     5941 caaaccagaa ctccatctca ccgaacattt tcacggtggc aaggttgagc gtcagcagca
     6001 gcactatcac cgccagcgag gcgacccagt cggagaaatc ggggaaccag aactgagcat
     6061 aagccgtgat cgccaccacg tctgccatac cggttacaac ccagcagaac cagtaagtcc
     6121 agccggtgaa atatcccgcc cacggcccca gtaaatcgga agcgaagtca ctaaaagatt
     6181 tgtattccag attcgaaagc agcaattccc ccattgcccg catcacgaaa aagagcataa
     6241 aaccaatgat catataaacg aaaatgatcg acggcccggc aaggctaatc gttttgccgg
     6301 accccataaa caacccagta ccaatggcac cgccaatggc aataagctga atatgtcggt
     6361 ttgtgagatt gcgccgtagc gactgttcag ccggagcctg atcatcggca acgactttta
     6421 cctgatctac catgtttttt cttcctgtac ctgtctgtgt tgttcaggct ctacggcctt
     6481 tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg taacaatacg
     6541 cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg agtgtcatat
     6601 cacggtgata acgcgaaaca gctcacaaaa atacacgctt acggggtctt tgcaacacaa
     6661 aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc ctctggttcg
     6721 aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa caccagggtg
     6781 ctgaacggag ggatggatgc acctgcgccg cgctcgccat atgccagttc ctgcgggata
     6841 gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca gccagggatc
     6901 acgccattaa ccgggaattc agcgggttca ccacgagcaa cggagctgtc aaacacggtg
     6961 ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc cggaattgcg
     7021 ccttcaccct ggttgatcac gcggaattgc aggccagatt cggtgctatt cacaccttct
     7081 tttttggcgt tttcttccag gtatttcaca ccttcagcag ccatcgcctg gaaacgctga
     7141 cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac cacatcaacc
     7201 ggcacagccg gatgtttgcc ttccagcgca tcggcaatac ctgcaaccag tgcttctggc
     7261 agcagccctt ccaggccaga ttcactcagt tgttgcccta cctgcaaacc aatgccgtag
     7321 cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc tttcttaggg
     7381 tataaaagta gcgggcagca tatcagccac acttctttgg gtaaaacttt gtcgcaggaa
     7441 tgatgacaaa tcccggggaa aaagaaacaa tagcaatatc ccgtatgaat at
//
LOCUS       DAJTYG010000144         7447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTYG010000144 DAJTYG010000000
VERSION     DAJTYG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.144"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            215..291
                     /locus_tag="OO568_003606"
     tRNA            215..291
                     /locus_tag="OO568_003606"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:249..251,aa:Arg,seq:tct)
     gene            complement(337..1098)
                     /gene="envY"
                     /locus_tag="OO568_003607"
     CDS             complement(337..1098)
                     /gene="envY"
                     /locus_tag="OO568_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6642781.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1281..2171)
                     /locus_tag="OO568_003608"
     CDS             complement(1281..2171)
                     /locus_tag="OO568_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6642782.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2172..5144)
                     /gene="nfrA"
                     /locus_tag="OO568_003609"
     CDS             complement(2172..5144)
                     /gene="nfrA"
                     /locus_tag="OO568_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCR6642783.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKAGDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTGAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPTNLPRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRELGNNAQYWWLHAQRYILAQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5131..7368)
                     /gene="nrfB"
                     /locus_tag="OO568_003610"
     CDS             complement(5131..7368)
                     /gene="nrfB"
                     /locus_tag="OO568_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR6642784.1"
                     /translation="MDWLLDVFATWLYSLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQAQAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
BASE COUNT         1734 a         1934 c         1948 g         1831 t
ORIGIN      
        1 tttccgttag ggcggtaaat tgtactttga tacatgaaaa tacgggtttt cttgattcag
       61 acgcgcagcg gtgtgcgttt gtttgccgct atagcgaaat aaatcagaaa atcagacgcg
      121 gtcgttcact tgttcagcaa ccagaccaaa agccattgac tcagcaaggg ttgaccgtat
      181 aattcacgcg attacaccgc attgcggtat caacgcgccc ttagctcagt tggatagagc
      241 aacgaccttc taagtcgtgg gccgcaggtt cgaatcctgc agggcgcgcc atttcttctt
      301 catttatgcc cgtcttatcc gtttccgctt tgcccttcac cacatcactt tttgtcgctg
      361 tttggcgaga taattcaacg gtgtcaggct gtaaaacgcc ttaaaaacag agataaagta
      421 cgacgtgctg ctatagccac ataattgcgc cacctgagtg atatttttgt tatccatcaa
      481 taacatctgt acggcgtaac gcatacgaca ctctgtgaca atctggctat agctggtatt
      541 ttcgtttttt aatttctttt tgagcaggct ggggcttaaa catagcgaac tggcgacaat
      601 tcgcagattc cagtaatgct gaatatcgct ttgaataatg cggcagacgc tgtcgcggac
      661 gctgctgcgt aagatataca tcagtagggc aataaaccgc gattgctcaa gaaagttaga
      721 caatacggta aaaagtaatg cgcgcgtcaa ctccgcttcg cagggtttgc tgggttgctg
      781 cttgctgtgg ttggcggcta gccggaacac ttccggtgta cggctatgac aagcgataac
      841 cggggttgcc agccgcgacc agggctttac acaggttaat ttcttattta aaaacaacag
      901 ataatcgttg atgatgttac ggttgatgtg ggtgattaat acgtgattta acgttgagag
      961 ttcaatgacg ttattgttgc acgcgaaggc ggccaggtag tttttcggaa gggtaaacgt
     1021 agcatggtta ttgacgctta cctctacctc tttttcggtc aggatcacca cgcaaggttc
     1081 actgctgctc aattgcattt cgcactcctc agatatcaga aactccgctc aaaggatcta
     1141 tgcttcctgc atgagtgatc ggcccgttcg ccgataacga tcttctttct ttagcatgct
     1201 ttttagcaat taatcttgat ggaattctga cgagagcgaa agaggtaagc caggtcgtac
     1261 ccgacttacc tggaggggat ttaatactcg agaatgccgt gcgcgacggg caaatagcgc
     1321 agagagaaat agagcatgtc tttaccgcaa gaggaacgtt tcgctaacag cgaggcaatt
     1381 tctgcgtccg gtttcggttt cgctgtaaag gttttgcctt tgccggcgac aaacagttca
     1441 taaacaatgc cgcttgctgg ggcggagctt tggcaatcgg cgctggcctg tagataacgt
     1501 tcacgctgtt cagcggtaag tttatccacg ccgctgccat cctgtaccca gacattaact
     1561 ccggttgctt taacttgttc cagcagttgg cgatagccat cgggcgacat gtttccggcg
     1621 aaaaaactac tgatataaac cggttttgct gaaacatcgc taatcagccg ctgcgcgttg
     1681 tttaaccatg ttagcaaagg ttgacgggcg gcttcgctgc gccagttcag gtcgtcaatt
     1741 tccgcgctga tgtaccagcc atccggcgtt atgccaggcg cggcgctcca taatctggct
     1801 tgctggagat cggcagccag caggcgatta agatagcttt ccagcgctgc agacgactgt
     1861 ttctggtgca taaaaaattc cggatcggcg ttcagcccga caatcagctt caggccagtc
     1921 tgttgcgcag ctgcggcacg cttaaacaat aacgtgcgct gttctggctg ggtaaatgca
     1981 tcgccgtaac gggtccattg caaaaccagg gtatcgaagc cttgaaaacg taactgactc
     2041 atcagcccct gccactgggt atcggtaacc tgactatctc ggttttgtgg ttgccagata
     2101 atacctttca tcgcaaagga aaaagggctg accaaaagca gtgtcagcaa tacgaaaatg
     2161 aacttacgca tttaccagtg cactccaatg gtgagaaacg cgttgttgcg ctctccgtta
     2221 cgttgattaa tcgccttaaa ggtatgttga tactcgacgc cgagactgac tttgtgcggc
     2281 caggcgtcgt agtgcgtctc gccggtccaa atattccagc ggaccccgac accgccaagc
     2341 tgcgcgccct gagtgccttt atcacgatag ccgttgtcct gaacgtgagc gtaaggctca
     2401 atagtctgtc cgttagctac cttctgatgc cagctgacgc gataatctgc cgtccacgcc
     2461 tgaatatcct ggcggatata ttgcgccgca tcgaggtaca ggttttgggc aaaccagcct
     2521 gaaccgttcg ggtgccattc gtcgctgtat ttgccgccat taaagaatga ggcgctggcg
     2581 cgcagcatgg tatcggatgc gccattttgc ccgttcagcg gcaactgctg ttcgacggcg
     2641 aggaaaaaga tctgatcgcg cagcggcttc cagcgcagac cggtgccgga catcggattt
     2701 ttcaccggca tcatcacccc gttttctccg gtatcggcaa agacgcggct ataaactgag
     2761 agcaggtcgc cttccagcag catattgcgt ccgatgcggt actcggcttc cagttgtccg
     2821 tagctacgat agcttttccc aggcgctgca ccgccgacat tattgttagc ggtactcatt
     2881 gcgccggaac gcaagccgat ggaagaatcg aaactgaacg tccagcggcg accgacctcc
     2941 tcatgcaaac ggcggaaatt gaagcgttgc tgattttgct ctggggtcag tggggttatc
     3001 agcgcctgat tatcaatgtc atcaatcacc agccgggcgt agtgctgcgt cgcaggcatg
     3061 tcatccagac gctggttcac gtaggccagt tgtcggatca gtgccggatc gtccggaagc
     3121 cctttatgcg cctgttcgag catttcccgc gactgtgcga tatcaccgct atcccacaag
     3181 gcgtaaccaa gcgctgcctg ggtgttgcta ttattcggtt ccagttccag cgcggcgcgc
     3241 aaatcactca ccgcggccgg aacattatga cgttggcgat aaattgtcgc ccgcgcaacg
     3301 taagcgttgg cagaaggggc aatattgatt gagcgcgtga gatcgttcag tgcgagttct
     3361 ggctgagcta ggatataacg ttgcgcatgc agccaccagt attgagcatt atttcccagc
     3421 tcacgttgtt ctgcctgttg aagccagcga tcgcgagccg cgccatttcc cgctgcctga
     3481 gcggtgttgg cggcggcgag cagatcctca ttgctcatgt cgtgaagact gattttctgc
     3541 caggccgcca gtgcggtggc gtagtcctca acctgatacg cctgataggc taccgcacga
     3601 tgttgccagg cgctcggttg tcgttgttcg gcctgaagcc atgcatacaa cgccacaccg
     3661 ggtagcgtgt cccgataaca ctttgccaga cggttccagg cggcagcatc gtaggaaggc
     3721 gacatatcgc ccagcaagcg aactattgcc gggcaattat ctgcaatacc cggcaactga
     3781 ctttgccact gacgttgctc cgccagcggt aagggtttcg ataaaatcgc caccttcgcc
     3841 ggcgttgcta ggtaaggatg actttccagc agagacgcca gtcgcgccat taaagtctgg
     3901 ctgacacgcg catcgccctg gaaaggatag cgttgcagca ataaatcggc agcttcgcgt
     3961 gactgctcgt tctgcatcag ttgccaggtc agttgatcca ggcggggaag atttgtcggt
     4021 tcttgctgat acagcaatcg tgccagacgc agagattcag ccttgttacg ggtcgccacg
     4081 ctgacagcat aacgctcctc aagcatttca ttggcgggga gggtggcgag cagtttttgc
     4141 gctgcgtcgt actgaccttc ttttaacagc accggtagcg tcgcgccaac aacatactgg
     4201 cggttatcgg caaactgcac cgtataattc gccaatgcct gaacggggtt ggcgctgtat
     4261 ttagataaca gatagagcca acttttctct tgtgcgcccg tggtaaacag cggcttattt
     4321 tcaatgagat aatgctggag gcgtgctttt tcgccacgat aagccagcgc ggtcgcgtaa
     4381 gtaatatatg actgaggatc ggtgaagatc ccctgtgatt gcagtgccag gatccgatcg
     4441 tccagctgcc cggcaagaag cacgtcaaac cactgacggc gttctgccgc gcttaatgtg
     4501 ttctgctggc gtgcttcatt gtatagcgta tctgcctggg accattgttt caggtagatt
     4561 gcccgttgca gcagatcggt tcgcagcgtt tttccttccg gcgatgcagc aaacgtcgca
     4621 tcgttcagtt gcgctctggc gacaggtaat tgtgccagcc gcagggcatt ctgcccgact
     4681 tcactgcgac aacgcagggt cggcgcagcg tcgcccgctt tttgctgggc aagcagttct
     4741 tcaacagtcg taacgctttt cacttcaact ggaatagccg ccagactgcg ctcaagtcgg
     4801 gcatctcctg ggtgacgttt cagttgatcc tcaagcaaca gccgcgcccg gtcatcatga
     4861 ccaaaatgac gataggcttc cgcaaggtat aaagtcagcg gaatattatc cggcacctgt
     4921 tggtgtatat attcaaattc gcggatggcg gttgcttcgt cgttattttt ctgtgccttc
     4981 agcgccttat cgagacgggg ataaataaca aaatggcgat aatcgctcag ccccagctct
     5041 tctgcgctgg tgccgatatt gtctgcgagt gcgctggtac tcaataaaga cgtcagcagt
     5101 aaaccagacc atccgatgac gcgattaagg ttattctcct tcattttcgg actccagttg
     5161 cgcaacctgt tctgtgttta aacctgcttt gagtaatagt gattgcatcg aaacttgtaa
     5221 ttcgcgttga attgtcagga cgcgatccaa cgtttcctgg ctgataacgc cttcggtgac
     5281 caaaaacttg ccgagcggca gagaactgcg ttcatggcgc aataacaaca cgttaattgc
     5341 tgaacgatta atatgaccga gcgtggtcag tatttcggcg aacaggaact gatgcggcac
     5401 atattgccgc cagatttcac cggtctgctg ttccgtgagc cactgatgct gaaccgcatt
     5461 gtacaacatt gcccgcggat cgtgaccgcg tcggcgtgca taccagtggc gtaaccctgt
     5521 gacaatttgt ccccgcagaa caatgacgta acgcactttg cgtccgactt tacgcgtcag
     5581 ggccgccagc gaaaccgggt caataccatc ttcactgccg acaattaact catcattttc
     5641 cagacgcagc ggcagtactg cataatgcag cgctacggag gctggcattt cggcaatcag
     5701 cgaggacggg atctgccagg catcgatgga ttcccacgcc acgccgtttt gctctgcctg
     5761 cgcctgtgcc agctgctcgg cgctaatcag cccctgcatc agcattgaac cgcccaggcg
     5821 tagaccttcg acgcgattac gcagtgctgt atcgagttgt tcttcagtga tgacctgatt
     5881 ttccagcaga atttgaccta acgggcgcaa cgagcgggta tcgccagtga cgctggggaa
     5941 gtcatgcgtt gttttatccc acgccacgcg acgcggatcg ccgtgctgaa gaacctgttt
     6001 cagcacgcgc cagttagcca taaagttaat caggttgccc cagaaaagac gcaggacgga
     6061 aagcaggccc tgcgtgaggc cgtagtagcc agtgacgaaa atcactcgct gcacgatgcg
     6121 gttaaccatc aagccaaagt ttagccacag cagggtcatt aaccatgcgc tgccgctaaa
     6181 aatagaaagg aaatgccagg catcgggcca caaactttca tacgccagca acagcaaaag
     6241 ctggatcatc accagcatcg cgaggaagct gacaaagtta ctgattgccc ctttgcggtc
     6301 gcgccagaga aagtagttca gcgtcaggct ggaggtccat ttatgggttt taaagccctg
     6361 gaaaacaatg ccgatgatcc agcgagattt ttgtcgaacc gcagtcgaaa aggtatcggg
     6421 gaaatattcg cgcacgcaga tcatgtttga cgtccgtgcg tgctgtaaaa atttacgctg
     6481 ctcgcgttct ttggcttcgt ccaccaccgg aaaacggaca aaaatttccg tcataccttt
     6541 ttctttcagg cggaagccaa tgtcgtaatc ttcagtaaga ctctgcacgt cgaaagcaat
     6601 accgttaccg tcagctaaca gtgcggtcac ggcgcggcgg ctgaaacagg tgccgacgcc
     6661 tgcgctgggc acttgtccgg cgagggcttc acgcaccgga acatctttgc catgcagttc
     6721 tgaaaactca tcaatgtaag tcatgctggt gaagtgcgtc cattcgcgtt caaacggata
     6781 cactgggatc tgaatcagat ctttacgctc gaccagatag ttgaacagac gcaattccat
     6841 cggtgaaatc acatcttcgg cgtcatgcag aataaaacca gcaaaagcga aattggcgct
     6901 acgttcaaac tgggtgatgg cgtccagcac gttgttcaga cagtcggctt tgctggtggg
     6961 accaggacgc gcgcagacta ccttatgcac gttcggaaag cgagcgcaca cttcgtcaac
     7021 atcacgttga gtatcggggt cgttggggta ggtgccaaca aagatatgat agttttcgta
     7081 gtcgagcgtg gtcgccgcca gctcggccat attgccgatg acgcccgttt cattccacgc
     7141 cggaaccata atcgctaacg gtttttcatc tggtttatac agttcgcggt aactcattcg
     7201 cgggtagcgg cgataaacac tcaacttgcg tttaatgcgg cgtacccagt agacgacatc
     7261 aataaaaaaa tcgtccagcc cgctgatgaa catgatgacc gctaacgtta tcgcgattac
     7321 ttttaagctg tagagccagg tagcaaaaac atcaagaagc cagtccacac aaaaacctta
     7381 cattaacgct ggttatgttt agggtggcgt atattaaggt tttttatgaa ttgtgacagc
     7441 tttttac
//
LOCUS       DAJTYG010000145         7369 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTYG010000145 DAJTYG010000000
VERSION     DAJTYG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.145"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..207)
                     /locus_tag="OO568_003611"
     CDS             complement(<1..207)
                     /locus_tag="OO568_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR6642785.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQS"
     gene            727..1422
                     /gene="minC"
                     /locus_tag="OO568_003612"
     CDS             727..1422
                     /gene="minC"
                     /locus_tag="OO568_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6642786.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAGRL
                     QLVENALTVQPLN"
     gene            1446..2258
                     /gene="minD"
                     /locus_tag="OO568_003613"
     CDS             1446..2258
                     /gene="minD"
                     /locus_tag="OO568_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6642787.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2262..2528
                     /gene="minE"
                     /locus_tag="OO568_003614"
     CDS             2262..2528
                     /gene="minE"
                     /locus_tag="OO568_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6642788.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2900..3244)
                     /locus_tag="OO568_003615"
                     /pseudo
     CDS             complement(2900..3244)
                     /locus_tag="OO568_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3279..3398)
                     /locus_tag="OO568_003616"
     CDS             complement(3279..3398)
                     /locus_tag="OO568_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6642789.1"
                     /translation="MPLSYAGYHHFIMMSLVSAEHNTPALSLQDESPGLVPAG"
     gene            complement(3359..3544)
                     /locus_tag="OO568_003617"
     CDS             complement(3359..3544)
                     /locus_tag="OO568_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642790.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3645..3818
                     /locus_tag="OO568_003618"
     CDS             3645..3818
                     /locus_tag="OO568_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642791.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3820..4164
                     /locus_tag="OO568_003619"
     CDS             3820..4164
                     /locus_tag="OO568_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6642792.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4168..4503
                     /locus_tag="OO568_003620"
     CDS             4168..4503
                     /locus_tag="OO568_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6642793.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4560..6881)
                     /locus_tag="OO568_003621"
     CDS             complement(4560..6881)
                     /locus_tag="OO568_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6642794.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINSQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LYLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTETTNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6998..7207)
                     /locus_tag="OO568_003622"
     CDS             complement(6998..7207)
                     /locus_tag="OO568_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642795.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         2020 a         1735 c         1641 g         1973 t
ORIGIN      
        1 tgattgtaag ttttcagcgg ctttttcgcc gagatatttc cttgttaact ctggggaaat
       61 gccatctgag ttcacacaaa tatacgggtc gcaaacaacg ccgcacgccg gagattgaag
      121 tccgccagcg tcatgtccag acgccattga tgagaacatc atccccacac ctgacaatcc
      181 gatataaata atacgcatca tattcatagc acatcctgat aatccaaccg tacaacactt
      241 atgctcattt cgtcatcaat ccgtaatcaa aaccgctgac ctggacgagt tacgatgacc
      301 tgacagaagt atcagacgtc ttctgcggtt tctatgatag agcaattctg tggcggatat
      361 taagataacg ggtatctgtt ctttaatgat gctgcaaaaa atggaaagtt aagaaagcct
      421 ggcgtggtga aaaaatacac ttgcctcaat actggatatt taaacaggtc tggagatgct
      481 gtcacgatta gggtgcggaa aaaggaagcg taaataagaa gaatcaaaag tacgacggca
      541 atgggttgat tgacaagggt attttttaag ctatgaatca gcgccattta tcacagaata
      601 gacttttact ctgaataaat gggagggtga cttgcctcaa tataatccag actataacat
      661 gccttatagt cttcggaaca tcatcgcgcg ctggcgatga ttaatagcta attgagtaag
      721 gccaggatgt caaacacgcc aatcgagctt aaaggcagta gcttcacttt atctgtggtt
      781 catctgcatg aggcagaacc taaggttatc catcaggcgc tgaaagacaa aatcgctcag
      841 gcccccacat ttttaaaaca tgcccccgtt gtactcaacg tcagtgcact ggaagacccg
      901 gtaaactggt cagcgatgca taaggcggtt tcggcaaccg gtttgcgggt tattggcgta
      961 agtggctgca aagatgcgca acttaaagcc gaaattgaaa aaatggggct gcctatcctg
     1021 acggaaggaa aagaaaaagc gccacgtcca gctcccgcac cgcaggctcc agcgcaaaat
     1081 acaatgccgg tcacaaaaac gcgtttaata gataccccgg tgcgttccgg tcagcgtatt
     1141 tatgctccac aatgtgatct gattgttaca agccacgtta gcgctggggc cgaattgatt
     1201 gccgatggga acattcatgt ctatggcatg atgcgcggtc gtgcgctggc aggggcaagt
     1261 ggtgaccggg aaacgcaaat attttgtacg aacctgatgg cggaattggt gtccatcgca
     1321 ggtgaatact ggctgagtga tcaaatccca gcagaatttt atggcaaagc ggggcgactg
     1381 cagttagtcg aaaacgcttt gaccgttcaa ccgttaaatt gatccctttt taacaaggaa
     1441 tttctatggc acgcattatt gttgttactt cgggtaaagg gggtgttggt aagacaacct
     1501 ccagcgcggc catcgccact ggtttggccc agaagggaaa gaaaactgtc gtgatagatt
     1561 ttgatatcgg cctgcgtaat ctcgacctga ttatgggttg cgaacgccgg gtcgtttacg
     1621 atttcgtcaa cgtcattcag ggcgatgcaa cgctaaatca ggcgttaatt aaagataagc
     1681 gtactgaaaa tctctatatt cttccggcat cgcaaacacg cgataaagat gccctcaccc
     1741 gtgaaggggt cgccaaagtt cttgatgatc tgaaagcgat ggattttgaa tttatcgttt
     1801 gtgactcccc ggcagggatt gaaaccggtg cgttaatggc actctatttt gcagacgaag
     1861 ccattattac caccaacccg gaagtctcct cagtacgcga ctctgaccgt attttaggca
     1921 ttctggcgtc aaaatcacgc cgcgcagaaa atggcgaaga gcctattaaa gagcacctgc
     1981 tgttaacgcg ctataaccca ggccgcgtaa gcagaggtga catgctgagc atggaagatg
     2041 tgctggagat cctgcgcatc aaactcgtcg gcgtgatccc agaggatcaa tcagtattgc
     2101 gcgcctctaa ccagggtgaa ccggtcattc tcgacattaa cgccgatgcg ggtaaagcct
     2161 acgcagatac cgtagaacgt ctgttgggag aagaacgtcc tttccgcttc attgaagaag
     2221 agaagaaagg cttcctcaaa cgcttgttcg gaggataagt tatggcatta ctcgatttct
     2281 ttctctcgcg gaagaagaac acagccaaca ttgcaaaaga acggctgcag attattgttg
     2341 ctgaacgccg tcgcagcgat gcagaaccgc attatctgcc gcagttgcgt aaagatattc
     2401 ttgaggtcat ttgtaaatac gtacaaattg atcctgagat ggtaaccgta cagcttgagc
     2461 aaaaagatgg cgatatttct attcttgagc tgaacgtgac cttaccggaa gcagaagagc
     2521 tgaaataagc ccgctgtaaa aacgcattta tcttcaaggc agagtcattt ctgccttgag
     2581 tttttcatcc ctctcatcca cgttgtggta aagcggcgag tattcttgct gatactcctc
     2641 attgctattt ccacctcccc ctttacacct taaggctgtt cccacgcaca gaacaaaatg
     2701 tttatgcctg gttgagtaaa taaccatgtt gttagtgtgg atatactggg agatgataac
     2761 cccgcaattt cattattcat tgttctccca taggatgagg tttcccgccg taactggcgg
     2821 gctttttttg ccccagaatt ttctctttca acatcctgta aacgaaaact gcgccgaagc
     2881 gcagtttaat cgttgatggt cagaacgacc agttcacacc agccaccgcg ttccacgggg
     2941 actccacacc ggcaccatgg ctatacccca ccccgaaatg cccgcttaac gtactgctga
     3001 atgaggcttt aatacctgcc tggtatattc cacgtctgcc cgacaaatca ttgacaaaat
     3061 taccgtcact attcactttc acccgattat catcgacaaa ttctttgcgc acagccgcct
     3121 tcaggcaggg ttcaatttcc ataccgttcc ccagacgcat gttgtaactc agcgttgcac
     3181 ccagttcacg atatatactg cgggtatcga ctgatttcga ttccatgcca ttggataaat
     3241 gatagtagcg tctaagtgac ctaagctctg ctgcaacatc agcccgcagg caccagacca
     3301 ggggattcat cctgaagaga cagcgcaggt gtattgtgtt cagcgctcac caaagacatc
     3361 atgatgaaat gatgatatcc cgcataagaa agaggcattt tttacacgca gtgcgctgaa
     3421 gtgtagttgg ataaaaaatc actcttttca gataatactg tcgtacgctt atctttagac
     3481 aatgaggccg tcggcaaaat aaaatgacta atgtaatcgt tgctgatgaa tatcttctgc
     3541 tcacataaaa atcacacaat aatattgata aatcagatta tcttaccttt acaacattgc
     3601 gttaacagtc aatgacactt cttgaataaa aatattgtca atcaatgagt tattcctcat
     3661 ttaagattat tctcattcac gtaaaaaaca taattcctat catcacagcc acgctcattt
     3721 tgaattatct gaataatagc gagcgttctt tagttaagca gatcttaatt gaagacgaaa
     3781 ttattgtgtg tgcaacttac ttaataccgg acatctaagt tgaagaaaaa aatacttgcc
     3841 ttcgggctta tctcagcatt gttctgctcc acgccagcga tggcggatat gaaccgcacc
     3901 acgaaaggtg cattgctagg cgcaggcgtt ggtttactta ctggcaatgg tgttaatggc
     3961 gtactgaaag gtgcagctgt tggcgctggt gttggtgcag taacagaaaa aggccgcgac
     4021 ggtaaaaatg cgcgtaaagg tgcaaaagtg ggtgccgccg ttggtgctgt cactggcgtt
     4081 ctgaccggca atggcctcga aggggcgatt aaaggtgccg tgattggcgg tacgggtggt
     4141 gcaattctgg gtaaaatgaa ataaggaatg atgatgaaaa aatttgcttt actggcgggc
     4201 ctgtttgttt tcgcaccaat gacctgggcg caggactaca atattaaaaa cggtttgccg
     4261 tcagaaacct atattacttg cgcagaagcc aacgaaatgg caaaaactga cagcgcacag
     4321 gttgcagaaa ttgttgcggt aatgggtaat gccagcgttg ccagccgtga tttaaaaatt
     4381 gagcaatctc cggaactgag cgccaaagtg gtagaaaaac tgaatcaggt ttgcgctaaa
     4441 gacccgcaaa tgctgttgat cacggcaatt gatgacacca tgcgtgctat cggcaaaaaa
     4501 taatatttca tatgtaccgc gtcgatgaat acgacgcggt tttttagcta acggcgggat
     4561 cagaacttat atttcaggcc caccatcact gcggtgtcat tgtagccatt atcgcccagc
     4621 tgcacgccca cattccccca gacgctggcc gctggattca ggttgccatt tacgccaagt
     4681 ttaatttccc ccaaattact caccccatct tgttcgactt tcacaccgtt cattgagaca
     4741 gcaaaatctt tactgttatg gatccagttc atttcaacaa aaggttcaaa gtcgtcaccg
     4801 tgcggaccgc tgtttttctc ctgagtacga ataaaggtgc ggaagccgag tcgggtctgg
     4861 atgttgttat ttgcgtcatt ctcaatacgt gagccattgg cttccgtgtg cgcattatga
     4921 tcgacgccca tccagatagc ctgtgcctgt ggctggaaga tccacgtata tttcgccgct
     4981 tccagaccaa aggatttatt cagggcaaaa ccgtaacccg cttccagcga gacggttgca
     5041 cctttcgcgc tataggattc ataggctaat tcatcccctt tcacggagtt tttaaaccag
     5101 ctatattgcg cccaggagtc aatgtatgcg ccttttttac tgatgtcatc ggcaaaccaa
     5161 gtggcataca gccctgcgct atagcctctg acactccctt tcgaacgata atccgacacg
     5221 ctggaatgag ttaagttgta gtcgcgggca taaccagcca tcactcccaa acgccaactg
     5281 tcactatcgg taaaaccacc ggttaacaga tcgcccccta actgcgagac gtagcgatgc
     5341 gaggtcgttc tcaactgtcc gttgctgtca cgccaggcat tatgcccgcc aatttgacgt
     5401 agccaaagtc ggcttgaacg ctcctgttca gttacaggat cgatgtagcg cgtttcaccc
     5461 gcacggtcgt tcagacgcat aacaaaaaga gtgttagcgg ctgccaggtt ggcgacgtaa
     5521 cttccagctt cggggcgcag gacgggggta ggcgcaacag taggcgctga acttcctccg
     5581 gagtttgttt caccgccgct cgttgcctgg aagttagtca gataccagtt tttattggtc
     5641 gtttcggtat tacctcgggt taatgaatat tcgtaggcac cggcgaaaat cctcgaatcc
     5701 ttaataaatt ccccatttga ttccccctca acgctgataa tctctatccc atttaacgtg
     5761 taagctccgc tgcctcctgc attggtaact ttaactcggg ttgttccaga ggtatttcca
     5821 ttaataacga ctttatccgt agctgaatta tcatcatcca attcagttct gagatacaat
     5881 acgccattat taccaacgta attttcagtt attgttaatc ttgttggttc gacatcccgt
     5941 ccatctgccc gggaaatata gaccgtacta ttatcaaccg tgaggttgct caccgttgag
     6001 tcagctttaa tatcccaggt actattatct gaaagcgaca gataggtatg agtgttatca
     6061 tctgttgaaa tattagctga acccgttatt tttgaattag ttgcatcgac ggtcaaatga
     6121 cctttaatat tattgataaa aacatctgca gccgatgata cctcactatc cgtaatggtc
     6181 acgttactcg tctcattttt atggtagagg atttcatttg ctgaccgtag tgagctacca
     6241 gataaaatat ctaccgttgc ttcctgacta tttatggaag actccagcac aattccagac
     6301 ccctgatgcg cgactgcaga tgtatttttc agcgtcaccg tgccgccatt ataaatttta
     6361 gcaatcgcgg cgtcaggacc cgttgtataa atttctccct ggttaaatga aactgtcccc
     6421 tggcgggcag cgatggcatg agcattgtcc ccatgcgtgg tgatatcaac atcggttaat
     6481 gtaacgctgg cattggtata atcaagcgtg tcaccagcct tgacgacagt gccattagcg
     6541 tacgcggcat aactttctgt tccgtgggtt gtgatctccc cttttgccat atccagtttt
     6601 gctatggcat tattttcatt acttcctgta tcggcagtac tttcttcatc aagagaagat
     6661 acacacgcac gcgcgcttcc gggagatgca cacgcaagaa cgccgactgc gtgattaccc
     6721 gcagtaacaa taaatgtatc atctccgtca gaaaaagagg gggtaaccga accgtcagag
     6781 ttgactctta ttatggtgtc agtattcagg tttccactcg tttgacttag caaaccaatt
     6841 gagtaatccc ccgttgtgac aatcgtgttt ttaccctgca aaataacagc accgccaaat
     6901 tcacttacga ttgcgctagc tgttaatgct gggcttagag gactattgtc agaaggatat
     6961 aaaccgcccg ttgttacaat gacgccattg ttaatggtta acttactacc ctctcctgca
     7021 acggataccc gtaacttcca ttacccgttg ttataactag cgcattatca atatctttta
     7081 cttttccatc ttttattgcc acagcatgtt tatattcatc gtgctgaata aactgttcag
     7141 catatgcatt acccaccgcc agagcaataa gtcccaaact aaacccgggg agttttttca
     7201 gtttcataga ttaaccatcc attcattgga ttaacaaaca ttcctggtca aataacctat
     7261 gagctcagat taccaaaacg gagggtaaag acaaaaacaa aagcacaaaa aatttaaata
     7321 gataaacagc gacaatatat aaatgataaa taagtcggca ttatcattt
//
LOCUS       DAJTYG010000146         7253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTYG010000146 DAJTYG010000000
VERSION     DAJTYG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.146"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            34..435
                     /locus_tag="OO568_003623"
     CDS             34..435
                     /locus_tag="OO568_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6642796.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVSAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OO568_003624"
     CDS             447..818
                     /locus_tag="OO568_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6642797.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..1395
                     /locus_tag="OO568_003625"
     CDS             805..1395
                     /locus_tag="OO568_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6642798.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            1392..1791
                     /locus_tag="OO568_003626"
                     /pseudo
     CDS             1392..1791
                     /locus_tag="OO568_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1802..2542
                     /locus_tag="OO568_003627"
     CDS             1802..2542
                     /locus_tag="OO568_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR6642799.1"
                     /translation="MTTPNPLAKTKGAGTTLWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYLNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVPLSGLVATVKG
                     VQQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            2601..2987
                     /locus_tag="OO568_003628"
     CDS             2601..2987
                     /locus_tag="OO568_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6642800.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRKAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2996..3325
                     /locus_tag="OO568_003629"
     CDS             2996..3325
                     /locus_tag="OO568_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6642801.1"
                     /translation="MQFVMRLAREFRRADWRQMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            3297..6338
                     /locus_tag="OO568_003630"
                     /pseudo
     CDS             3297..6338
                     /locus_tag="OO568_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            6338..6667
                     /locus_tag="OO568_003631"
     CDS             6338..6667
                     /locus_tag="OO568_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6642802.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIREVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            6667..>7253
                     /locus_tag="OO568_003632"
     CDS             6667..>7253
                     /locus_tag="OO568_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6642803.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGAGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKP"
BASE COUNT         1752 a         1662 c         2332 g         1507 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgctca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctctgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccgctgtc cggaccgctg acacaggcat ttgaagatgc ccgcgatcgc
     1321 atcattgctg cggaaatgcc gaaacagctg gggtatgccc tgaaacaaca actgaggtta
     1381 catctgagta aatgaaccgg cacacacaaa tccgtcagag cgtgctggca cgccttcggg
     1441 aacagtgtgg agacagcgcc acgttttttg acgggcttcc ggcatttatt gatgcgcaaa
     1501 ctgcctgccg tggcggtgtg gctgagtgat gctcagtaca ccggaaaaat gacggatgga
     1561 gatgactggc aggctgttct gtatattgcc gtcttcatcc gggcacaggc accggattca
     1621 gagctggata tgtggatgga gagcaccatt ttcccggccc tgaatgatgt accggcactt
     1681 tccggactca tcgacaccct gaacccactc ggttttaact atcaacgtga taatgagatg
     1741 gctacctggg cgatggcgga aatcacgtac cagatcacgt acacgaatta aggaggtggt
     1801 aatgaccaca ccaaatccac tggcaaaaac gaaaggtgcg ggaacgacgt tatggatgta
     1861 taccggcaac ggcgacgcat ttgcgaaccc tttgtcggac actgactggc tgcgtcttgc
     1921 gatggtgaag gatctgcaac ctggcgagat gaccgctgat gcagaagatg acacttatct
     1981 cgatgatgaa gatgcagact ggaaaacgac aacccagggg cagaaatccg tcggtgatac
     2041 ttcggcgacg ctggcctggc gtccgggtga cagcgggcag aaaaaactgg ttcagttgtt
     2101 cgattccggt gaagtctgcg cgtttcgtat caaatacctc aacggtacgg ttgatgtttt
     2161 ccgtggctgg ctgagttcac tgggtaaaac cattacctca aaagaggtga tgacccgtac
     2221 tgtaaaaatc agcggtgtgg ggcgtccgta tctggcagag gagggcgctg aaaccgtggg
     2281 tgttaccgga ctgacggtgg caccggcatc tgtcagtgtc aaagcgggag caaccaccac
     2341 actgaccttt acagtaaaac ctgacggggc cagtgacaaa gcgatcagtg tgcattcgtc
     2401 agatcctcag actgcttcgg tgcccctgag cgggcttgtg gccacggtga aaggcgtgca
     2461 gcagggcagt gtcagcatcg tgggcatgac ctctgacgga gagtttgtgg cagtggctgc
     2521 ggtgaccgtc agcgcaccat aacaggacga tactcatcat tgccccggtt atccggggct
     2581 ttttgcatcc ggagaacatg atgtttctga aacaggatac gtttaattat gaaaaacagt
     2641 ccgtggtgct cagtgagctg tccgggctgc agagaattga atatctgacg tttgttcagc
     2701 agcgaaaggc aaagtttgat gcacaggagg gagaactgcc ggaggctgaa cgacagattg
     2761 cttttctgcg tatgggaatg gatatcaatg cctggctggt ttcccgctca ctgtggaatg
     2821 ctgagcagtc tcaggatgtt gagacgcttt gcgcatccat tatgacaaca tggtcgtatg
     2881 atgcgctggg cgcgggggcg gagagggttc tgtcgctgag cggtatgggg accattgaga
     2941 atgccgggga tgatgatcat gaggcgctga cgccggaaaa gtcctgacgc gggaaatgca
     3001 gtttgtcatg cggcttgccc gggagttccg gcgggcagac tggcggcaga tgctgtcgga
     3061 tatgtcggcc actgagcttg gtgagtgggg cgattatttc cggatgcaga gcttcagtga
     3121 tgtgtggatg gatgcgcagt ttgcctcgct gaaggcattg atcgtgagaa tggtgtccgg
     3181 cagcagcgat gctgcggtgg ctgatttcag ccttttaccg gaagagaacg ggataccgga
     3241 gcgaacggac gaagaactga tgcatcttgg ggaaggtatt tccggaggtg tgcgttatgg
     3301 accagatagc caacctggtc attgatttgg ggattgatgc ggcagagttt aaaaatgaaa
     3361 ttccccgtat caaaaacctt ctgaatggtg cagccagcga tgcagaacgg tcttctgccc
     3421 gtatgcagcg ttttatggag cgtcagactc aggcggcccg gcagacaatg caggcggctt
     3481 cttcggctgc aacagcagca tcagcccatg cgcagacggt ggagaagaat gcacgggctc
     3541 atgaacgcat ggcccgtgag gtggaacaaa cccgtctgcg cgtggatgcc ctgaatcaga
     3601 aaatgcgcga ggaacaggcg caggccaggg cactggcgga ggcgcaggat aaagcggctg
     3661 ccgcctttta tcgccagatt gacagtgtga aacaggccgg tgcggggctt caggaattac
     3721 agcgtattca gcagcagatc cgacaggcca gaaacagtgg cggggttggt cagcaggatt
     3781 atctggcgct gatttcggag atcacggcga aaacccgtgc cctgacgcag gcagaggaac
     3841 aggccacccg gcagaaagcg gcgtttatcc gccagcttaa agagcaggca acccgccaga
     3901 atctgtcgtc ttctgagttg cttcgcgcca gggcggctca gctgggggta agcagtgctg
     3961 cagaagtgta tatccgcaaa atggagcggg caggaaaagc cacacattcg ctggggctga
     4021 aaagtgcggc agcccggcgg gagctggggg tgttaatcag tcagatggcg cgcggcaatt
     4081 ttggtgcgct gaggggatcc gggataacgc tggctaaccg tgccggatgg acaggcgcac
     4141 tgatgtcgcc gaaaggcatg atgactggcg gcgttattgg cggacttgtc gcggcggtcc
     4201 tgggtctggg taaagcctgg catgacggcg gaaggagggc gaggaattta accgtcagct
     4261 ggcgctgacg ggacattatg ccggtgtcac tgtcgggcag ttgtggaaac tcagccgggc
     4321 catatccggg aatggtatca cgcaacatgc ggcagccggt gcgctggcac aggtggtggg
     4381 cagtggggca tttcatggaa acgatattgg tatggtggcg aaagctgccg cacagatgga
     4441 gcgatcggtt ggccagtcgg tcagcgatac cataaatcag tttaagcggc tgaaggatga
     4501 tcctgtaaat gccgcgaagg cgctggacaa tgcgctgcat tttttgactg ccactcagct
     4561 tgagcagata cgcgtccttg gggagcaggg gcggtccagt gatgcggccc ggatcgccat
     4621 gtctgcgctg gcagaggaaa ccggtaaacg cacatctgat attgataata atctcaatgc
     4681 gctgggtagt acgctgcaaa ccttgtctga ctggtggaag cagttctggg atgcggccat
     4741 gaatattggt cgtgaagact cgctggatgc gcagattgat gcgttacagg aaaaaattca
     4801 gcgcgcgaaa aaatatccgt ggacaaacgc ctccacacag gtggagtacg atcagcagcg
     4861 tcttaacgat cttcaggaga aaaaacgccg gaaggatttg caggatgcaa aagcgcaggc
     4921 agaacggaat taccaggagc aacagaaacg ccggaatgct gaaaatgccg cgctgaaccg
     4981 gatgaatgaa acggaagctg cacgacatca gcgggaaatt gcgcgtatta atgccatgca
     5041 gtacgccgac caggctgtca gggatgcggc gatacagcgt gaaaatgaac gttacgaaaa
     5101 agccattaag aaaaatacac gggcaacccg taatgatgag gccacccggt tattgctgca
     5161 gtacagtcag cagcaggcac aggtggaagg gcagattgct gctgccagac agtcagcagg
     5221 cattgccact gaaaggatga ctgaagcgca taaacagctt ctggccctgc agcagcgcat
     5281 cagcgacctg gacgggaaaa agctgacggc agatgaaaag agtgtgctgg cccgtaagaa
     5341 tgagctgatt caggcgctga cgctgctgga tgtgaaacag caggagctgc agaaacagac
     5401 agcgcttaac gacctgagaa aaaaaacggt tcagctgacc agccagctgg cagacaaaga
     5461 acgtgcactg cgtgagcagc acaatctgga tattgccact gcaggtatgg gggataagca
     5521 gcggcagcgc taccaggcac agttgcgcat ccggcaggaa taccggcaac agttgcaaca
     5581 gcttgagaat gacagtcgcc agaaaggcac ttacgggacg gaggactacc ggagggctga
     5641 ggaggtgctg aaggggagcc tgaagcgaca actgaatgaa aacaaacgct actggcagga
     5701 actggaagtg gcgcagggcg actggaaaaa cggtgccatg cgggcgtttc agaattttac
     5761 ggcggatgcg gataatgcgg cgggaacggc agaacagatg tttacagtgg cattcagcag
     5821 tgccggtaat gcactggcga cattctgtac caccggtaag ctgaatttta aatccttcac
     5881 ctcttccctg ttgtcagata tggccagaat tatggcacag atggccatga tgcaggcggt
     5941 aaagggcgtc ggttctttat tcggcttcac gactaatgct gatggcggtg tttaccagtc
     6001 tgctgatttg agtcgctaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc
     6061 aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgcgg
     6121 tgctgatggt aagctggggg gttgtggcgg atactggtgg ttcaggcatg gtgatgtttg
     6181 ccccgcagta caacattgag atcaataacg acggcacgaa cgggcagata ggtccggctg
     6241 ccctgaaggt ggtttatgac ctcgggaaaa aagcggccgc ggactttatg caacagcagg
     6301 cccgtgatgg tggccggtta agtggagcat accggtaatg gagacgtttc actggaaagt
     6361 gcgcccggat atgaatgtgg tatcagagcc gaaagtggtg acagtgaagc tgggcgatgg
     6421 ttatgaacag cgtcgtgcgg cgggactgaa taaccagttg tcgacttaca gcgtgacgat
     6481 acgtgttcgt aaaggtgaac acccatcttt aaaagccttt ctggaacggc acggtggcgt
     6541 ccgcgcattt cagtggacgc caccttatga ctggaaactg atcagggagg tttgtcgtaa
     6601 atggtcggca agcgtggggg cgttatgggt gactgtaacg gccgattttg aacaggtggt
     6661 gaactgatgc aggatatccg gcaggaaaca ctgaatgaat gcacccgtgc ggagcagtca
     6721 gcccgggtgg agctctggga aatcgatctg acagaggtcg gtggtgagcg ttattttttc
     6781 tgtaatgagc agaacgaaaa aggtgagccg gtcacctggc aggggcggca gtatcaggca
     6841 taccccattc agggggcggg gtttgaactg aacggcaggg gctgtgctgc ccgtccgaca
     6901 ctgacggtct ctaacctgca cggcatggtc accgggatgg cggaagatct gcagagtctg
     6961 gtcggcggaa cggtggtccg gcgtaaggtt tacgcccgtt ttctggatgc ggtgaacttc
     7021 gtcaacggaa acagcgacgc cgatccggaa caggaggtga tcagccgctg gcgcatcgag
     7081 cagtgcagcg aactgagcgc ggtcagtgcc tcttttgtgc tggccacacc aacggagacg
     7141 gatggcgcgg ttttcccggg gcgtatcatg ctggcgaata cctgtatgtg gacttaccgt
     7201 tctgatgagt gtggttacac gggcagggca gtggctgacg agttcgacaa acc
//
LOCUS       DAJTYG010000147         7211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTYG010000147 DAJTYG010000000
VERSION     DAJTYG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.147"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..329)
                     /locus_tag="OO568_003633"
     CDS             complement(<1..329)
                     /locus_tag="OO568_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642804.1"
                     /translation="MAYRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQ"
     gene            699..1649
                     /gene="corA"
                     /locus_tag="OO568_003634"
     CDS             699..1649
                     /gene="corA"
                     /locus_tag="OO568_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6642805.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1690..2070)
                     /locus_tag="OO568_003635"
     CDS             complement(1690..2070)
                     /locus_tag="OO568_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR6642806.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2084..2500)
                     /locus_tag="OO568_003636"
     CDS             complement(2084..2500)
                     /locus_tag="OO568_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642807.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2559..3449)
                     /gene="rarD"
                     /locus_tag="OO568_003637"
     CDS             complement(2559..3449)
                     /gene="rarD"
                     /locus_tag="OO568_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6642808.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3501..3968)
                     /locus_tag="OO568_003638"
     CDS             complement(3501..3968)
                     /locus_tag="OO568_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6642809.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4133..5002
                     /gene="pldA"
                     /locus_tag="OO568_003639"
     CDS             4133..5002
                     /gene="pldA"
                     /locus_tag="OO568_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6642810.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5135..6964
                     /gene="recQ"
                     /locus_tag="OO568_003640"
     CDS             5135..6964
                     /gene="recQ"
                     /locus_tag="OO568_003640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6642811.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7028..>7211
                     /locus_tag="OO568_003641"
     CDS             7028..>7211
                     /locus_tag="OO568_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCR6642812.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLH"
BASE COUNT         1852 a         1766 c         1805 g         1788 t
ORIGIN      
        1 tgctgaccgt tttcgatgta caaaccgagc ggcgcatagc tttcatcata gatgccgccg
       61 ttcatcgcca tctgcacctg accctgacta ttaatatccg ccagcagagc atgtaacgtt
      121 ccccacgctt cgccattggc tttttgccag tacattttca cccgctctgt ttgaggatta
      181 acggtatacg cctgtacggt cagcgtcgga tctgagagtg cgcaatcatc agcggcaaca
      241 gcaaacaagg gaagtaaggt gagggtgaga aaaatccgtt tgagattcaa ggtgatcatt
      301 cctttaccaa tgagtagccg atacgccatt ataggtcctg gatgtgggat ttttttatcc
      361 tgttagcgac cttgacgagt accaaaaagc gcgaagttca actattgttc tgtggtgttc
      421 tgttgcgtgt tgacggcaaa attttgctgg cgtaacatgc gcgcacgatc actctaagag
      481 gacattcgcc ttggacacac ccagtagata ctggctcact atcctgtcat ccaggatcaa
      541 ctcctaaggc tatccctttt tgctgatagc cttagcggtt gtcagcgacc ttaatttttc
      601 ccgtcgcgct gagtcaggct gtttaatggt ctgaaaccca atttgtttct gtgtgcccac
      661 cgaactgtcc gatattttaa gcattgggag tcccggtcat gctgagcgca tttcaactgg
      721 aaaataaccg actgacccgg ctggaagtcg aagagtcaca accccttgta aatgcagtat
      781 ggattgatct tgtcgaaccg gacgacgacg agcgactgcg cgtacaatct gaacttggcc
      841 agagcctggc aacccgcccg gaactggaag acatcgaagc atcggcacgt ttctttgaag
      901 acgacgacgg cctgcatatt cactccttct tcttctttga agatgcggaa gatcacgccg
      961 gtaactccac tgtggcattt accatccgtg atggtcgtct gtttactctg cgtgagcgtg
     1021 aactgcccgc ttttcgtctg tatcgtatgc gtgcccgtag ccagtcgatg gtagacggta
     1081 acgcctacga gttgctgctg gatctgttcg aaaccaaaat cgaacagttg gcagatgaaa
     1141 ttgaaaatat ctatagcgac ctggagcagt tgagccgggt gattatggaa gggcatcagg
     1201 gcgatgagta cgacgaggcg ctctccacgc tggcggaact ggaagatatc ggctggaaag
     1261 ttcgcctgtg tctgatggat acccagcgcg ctctcaactt cctggtgcgt aaagcgcgtt
     1321 taccgggtgg gcaactggag caggcgcgtg aaatcctgcg cgatatcgaa tccctgctgc
     1381 cgcataacga atccctgttc cagaaggtga acttcctgat gcaggcggca atgggtttta
     1441 tcaacatcga gcagaaccgc atcatcaaaa ttttctcggt ggtatccgtc gtgttcctgc
     1501 cgccaacgct ggttgcttcc agctacggga tgaactttga gtttatgcca gaactgaagt
     1561 ggagtttcgg ctaccctggc gcgattatct ttatgatcct cgcgggcctg gcaccgtatc
     1621 tgtactttaa gcggaagaac tggttgtaaa aagaaggcgg tggcttagcc tggctaagcc
     1681 acctgttatt caaaggctcc aggtatttaa cccttttacc tctttctcat aaaaccattt
     1741 gttcgtgtta acagcaacat aggctgctac ggcaattccc agaatgttaa cgccaattaa
     1801 tgcaccaaca aatagagata gaatgccaag taatagaact ataattgctt ttttccataa
     1861 gcccagaaca aataaataga tccaggaaca gaagaaagca atgaaattca tttgaatagt
     1921 taatcgttgt cttactttta atgctttaaa tgctgcttta tattctggtg ttgctcccca
     1981 gaatccagga aagccatgtt gatcataaaa attaaatcgg tatttccatt tttcacttaa
     2041 tgaatcatcg ttcatatatt ccttactcat aaatactcca taactattgt tttgatgaat
     2101 cagtaggtgc aagcattagc atactgaaag tggaaaaata acaaatcaaa aaaatcatcg
     2161 aaccattgcc tgaacaggca aaatcttcgg ctatcattgt gatgatagag atgatatata
     2221 ctgctaatgt accaaaaaca taaattttta tatatatgaa accactatca cggagtcgtt
     2281 ggcaattcat gttgatgacg agataatgga gtgcgatggt agagactata acaagaaaac
     2341 ctgcttcccc atcgttaaaa aagataataa gaaaggcaaa aatgaaattt attaaaataa
     2401 atgaaaatat atatcggcgt ctggaaatct taccgttaga tgttgggata aacatacgta
     2461 acataaattt tacatccttg tgtgagtctc ctgtcagcat ggcaatatgc ccactctcat
     2521 acaagagtcg gcatattttt tcagaatata tttatttttt atttggacgt tctacgctgc
     2581 gtataaatcg cgtccatcac aaaaattgcc agcgccaccc aaataaaggt gaaagtcacc
     2641 atcttatcgg cacccggttt ttcaccataa aacgtcacag ccagcaggaa catcagcgtc
     2701 gggccaatgt actgaaaaaa gcctaacgtt gagagacgca ggcgcgtggc agcggcagta
     2761 aaacacaaca gcggtacggt agtgacaata ccggcggcga tcagcagtaa attcagcgac
     2821 atcgggtttt gccccatatg gctggttgaa ctgtcggcaa tagcaaacag gtaaatcgcc
     2881 gccacgggca gcagccacat ggtttcaatt aacatgccgg tttgtgcttc taccgcaatc
     2941 ttcttgcgca ccaggccgta gaaggcaaaa ctaaatgcca gccccagcgc gataataggt
     3001 agcgaaccaa aagtccacag ctggaccaac acgccacata tcgccagaat caccgccagc
     3061 cattgcatcc ggcggaatcg ctcgccgagg aaaatcatcc ccagcacaat gttcaccagc
     3121 gggttaataa agtaaccaag gctcgcttcc agcatatggt gattgttcac cgcccagata
     3181 aacagtagcc agttgccacc aatcagcacg gcagagactg ccagcataaa aattttctgt
     3241 ggcgtctgaa tcagcgtttt taaataggac cactggcggc aaatgctcat cagcaccacc
     3301 ataaagaaaa acgaccagat cacgcgatgc gtcaggatct catcggcggg cacgtagtaa
     3361 atcagcttga agtatgctgg cgcaataccc caaataaaat aagcggcaag agcgagtaat
     3421 acgccctgcc gcgtttgttt tgcatccatc gggaatactc atttttaatt ggtaacagca
     3481 gtttacctgc ttttatgtct tcaacctacc atataggtgg cggtggcact ggcaatataa
     3541 agctgttctt cattgtgtaa ttcaacgcgg gcgacggcga ctttattgcc tgcacgcaac
     3601 aggctactgg tagcagtaaa acgctcgccc ctgcctgggc gcagataatc aacgcgaaga
     3661 tcaatggtcc ccatccgcga tagccgctgg cgtagttcat cttcactgat ggtttcgtgg
     3721 cgggttaagg tacttcccac gcacaccaga ccggcggcga catccagcgc cgacgcaatg
     3781 actccgccgt gcaaaatgct ttgcgcccag ttacccacca tcatcggctg atttttaaag
     3841 gccagctgtg cgaactcttt ttcgtaacgc tccagttcca tccccaatgc gcggttaaat
     3901 ggcatgtgat aaacaaacat ctcacccact aatttcaggg cttgttcagc ggtcagtacg
     3961 gcagacatat catccttaca cttcattggt taatgaaatg ttgattttat gcttctttgt
     4021 tgttggtttc tactttagga agggataact aacggcaatg gagttaagta tgtaaaatag
     4081 cccgcagaaa aacattcacc ttatcaataa ttcgttacgg agaacacgac cgatgcggac
     4141 tctgcagggc tggttgttgc cggtgtttat gttgcctatg gcagtatatg cacaagaggc
     4201 aacggtgaaa gaggtgcatg acgcgccagc ggtgcgtggc agtattatcg ccaatatgct
     4261 gcaggagcat gacaatccgt tcacgctcta tccttatgac accaactacc tcatctacac
     4321 ccaaaccagc gatctgaata aagaagcgat tgccagttac gactgggcgg aaaatgcgcg
     4381 taaggatgaa gtaaagtttc agttgagcct ggcatttccg ctgtggcgtg ggattttagg
     4441 cccaaactcg gtgttgggtg cgtcttatac gcaaaaatcc tggtggcaac tgtccaatag
     4501 cgaagagtct tcaccgtttc gtgaaaccaa ctacgaaccg caattgttcc tcggttttgc
     4561 caccgattac cgttttgcag gttggacgct gcgcgatgtg gagatggggt ataaccacga
     4621 ctctaacggg cgttcagacc cgacctcccg cagctggaac cgcctttata ctcgcctgat
     4681 ggcagaaaac ggtaactggc tggtagaagt gaagccgtgg tatgtggtgg gtaatactga
     4741 cgataacccg gatatcacca aatatatggg ttactaccag cttaaaatcg gctatcacct
     4801 cggtgatgcg gtgctcagtg cgaaaggaca gtacaactgg aacaccggct acggcggcgc
     4861 ggagttaggc ttaagttacc cgatcaccaa acatgtgcgc ctttatactc aggtttacag
     4921 cggctatgga gaatcgctca tcgactataa cttcaaccag acccgtgtcg gtgtaggggt
     4981 tatgctaaac gacttgtttt gatgaacggt tgagtggttg gcaaatctgg aatccagcat
     5041 ccaggattac cctctcagag actaaaagca ttgcagtttc tcgcgcaggc gctgaaaata
     5101 gcgcctgttt ttatttcagg caatcggggt gaatgtggcg caggcggaag tgttgaatct
     5161 ggagtccgga gctaaacagg ttttacaaga aacctttggc taccaacagt ttcgccccgg
     5221 ccaggaagaa attatcgaca ctgtgctttc cggtcgcgat tgcctggttg ttatgcccac
     5281 tggtggcgga aaatcccttt gctatcaaat tcctgcctta ttgctaaacg gccttaccgt
     5341 ggttgtttca ccgctgattt cgttgatgaa agatcaggtg gatcaactac aggccaacgg
     5401 cgtggcggcg gcgtgcctta actcgacgca aacccgcgaa cagcaacttg aagtgatgac
     5461 aggctgccgc accgggcaaa ttcgcttact gtatatcgcc ccggaacgcc tgatgctgga
     5521 taactttctt gagcatctgg cgcactggaa tccggtgtta ttagccgtcg atgaagcgca
     5581 ctgtatctcc caatggggcc acgatttccg cccggaatat gccgcgctcg gtcagttgcg
     5641 ccagcggttc ccgacgctgc cgtttatggc gctgaccgcc acagccgacg acaccacgcg
     5701 ccaggatatc gtgcgcctgc tggggctgaa cgatccgctg attcaaatca gcagttttga
     5761 ccgtccgaat attcgctaca tgctgatgga gaaattcaaa ccgctcgatc agttgatgcg
     5821 ctacgtgcag gaacagcgcg gtaagtctgg cattatctac tgcaacagcc gggcgaaagt
     5881 agaagacacc gctgcgcgcc tgcaaagcaa gggtattagc gcggcggcct atcatgccgg
     5941 gctggaaaat aatgtccgcg ccgacgtgca ggagaaattc cagcgcgatg atctgcaaat
     6001 tgtggtggcg acggtggcgt tcggcatggg catcaataaa ccaaacgttc gcttcgtggt
     6061 ccactttgat attccgcgca atatcgaatc ctattatcag gaaaccggtc gcgccgggcg
     6121 tgatggcctg cctgcggaag cgatgctgtt ttacgatccg gctgatatgg cgtggctgcg
     6181 ccgttgtctg gaagagaagc cgcaggggca gttgcaggat atcgagcgcc acaaactcaa
     6241 tgcgatgggc gcgtttgccg aagcgcaaac ttgccgtcgt ctggtattgc tgaactattt
     6301 tggcgaaggg cgtcaggagc cgtgcgggaa ctgcgatatc tgcctcgatc cgccgaaaca
     6361 gtacgacggt tcaaccgatg ctcagattgc cctttccacc attggtcgtg tgaatcagcg
     6421 gtttgggatg ggttatgtgg tggaagtgat tcgtggtgct aataaccagc gtatccgcga
     6481 ctatggtcat gacaaactga aagtctatgg tatgggccgt gataaaagcc atgaacattg
     6541 ggtgagcgtg atccgccagc tgattcatct cggcctggtg acgcaaaata ttgcccagca
     6601 ttctgcccta caactgacag aggccgcgcg cccggtgctg cgcggcgaat cctctttgca
     6661 acttgccgtg ccgcgtatcg tggcgctcaa accgaaagcg atgcagaaat cgttcggcgg
     6721 caactatgat cgcaaactgt tcgccaaatt acgcaaactg cgtaaatcga ttgccgatga
     6781 aagtaatgtc ccgccgtacg tggtgtttaa cgacgcaacc ttgattgaga tggctgaaca
     6841 gatgccgatc accgccagcg aaatgctcag cgttaacggc gttgggatgc gcaagctgga
     6901 acgctttggt aaaccgttta tggcgctgat tcgtgcgcat gtcgatggcg acgacgaaga
     6961 gtagtcagca gcataaaaaa gtgccagtat gaagactccg taaacgtttc ccccgcgagt
     7021 caaatgtatg ttgatgttat ttctcaccgt cgccatggtg cacattgtgg cgcttatgag
     7081 ccccggtccc gatttctttt ttgtctctca gaccgctgtc agtcgttccc gtaaagaagc
     7141 gatgatgggc gtgctgggca ttacctgcgg cgtaatggtt tgggctggga ttgcgttgct
     7201 tggcctgcat t
//
LOCUS       DAJTYG010000148         6999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTYG010000148 DAJTYG010000000
VERSION     DAJTYG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.148"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..78
                     /locus_tag="OO568_003642"
     CDS             <1..78
                     /locus_tag="OO568_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6642813.1"
                     /translation="SLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(347..1117)
                     /locus_tag="OO568_003643"
     CDS             complement(347..1117)
                     /locus_tag="OO568_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642814.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPLLPADGTQVSF
                     PYACEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVRDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1162..1596)
                     /locus_tag="OO568_003644"
                     /pseudo
     CDS             complement(1162..1596)
                     /locus_tag="OO568_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(1608..1829)
                     /locus_tag="OO568_003645"
     CDS             complement(1608..1829)
                     /locus_tag="OO568_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642815.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(1830..2513)
                     /locus_tag="OO568_003646"
     CDS             complement(1830..2513)
                     /locus_tag="OO568_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6642816.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2577..2888)
                     /locus_tag="OO568_003647"
     CDS             complement(2577..2888)
                     /locus_tag="OO568_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642817.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(2885..3787)
                     /locus_tag="OO568_003648"
     CDS             complement(2885..3787)
                     /locus_tag="OO568_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR6642818.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4227..5186
                     /locus_tag="OO568_003649"
     CDS             4227..5186
                     /locus_tag="OO568_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR6642819.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5186..5581
                     /locus_tag="OO568_003650"
     CDS             5186..5581
                     /locus_tag="OO568_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6642820.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            5764..6144
                     /locus_tag="OO568_003651"
     CDS             5764..6144
                     /locus_tag="OO568_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR6642821.1"
                     /translation="MTMATINARIDDDINNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6190..>6999
                     /locus_tag="OO568_003652"
     CDS             join(6190..6535,6535..>6999)
                     /locus_tag="OO568_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
                     /protein_id="HCR6642822.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCSGAPWRDLPERYGAWKTVYNRFNRWSKSGVINIIFNRLLSLLDANGFIDWSATALD
                     GSNIRALKCAAGTPKKHPDIDGDNGPGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAH
                     ESQFAQRLLDGIGVQRQNGSMKRRGHAVLADKAYSGRALRNELKNNGIKAVIPRKSNE
                     KMASDGRAQLDRDAYCNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLG"
BASE COUNT         1891 a         1815 c         1723 g         1570 t
ORIGIN      
        1 tcactgtcgt tctcaaaatc ggtggagctg catgacaagg tcatcgggca ttatctgaac
       61 ataaaacact atcagtaagt tggagtcatt acccaagtta ccgtctggtt ttgcgaagat
      121 tgattattga tgacatgaac ggcaaatcga cctgattaaa aacctaacag gggaaaatgg
      181 ctcactgaag ccgaggggat ttttaggatt atcaggtgcg gaactcatcg aggcgcgggc
      241 atcccttgcc cacagagacg ccggatagat ttaagcaagc ccaccattaa tcaaaaatca
      301 gtgttgcaaa aacgggggtg accatagatt ttccccgcca tcgtcatcat ttccggattt
      361 ttgccagggc atccgccatg ctgcggattt cggcccgggc ttcccggaca acttcccgca
      421 gaacaccacg gtcaaaccac cggcggggcg gttcatccgg tatacgcagc acatcgcagg
      481 ccagcccact ggacaggata tgctcaatac aatctctgac cagatacatt tccaccgccg
      541 aaaaacgtgc gccccggttc aggatggcat cgagcaccac gggcaggttt tcatcgtctt
      601 catcgagcca gcaggggtga tcgctttcct tcacgaccag gcgaaagcgg cgcgtctggc
      661 gggtcagcaa cgtctgaccg cttgtggtca gcgccgcccg gatacgccgc gcatcttctg
      721 ccccccggca actgacactg catactgcat cgcgcaccgc atcaagaact gccctgattt
      781 cgtcttcggt cagccattca caggcatacg ggaaagacac ctgcgttccg tccgccggaa
      841 gtagcggcag cgactgccac tgactgccgg acagaacaag ttccgctccg gttccggtca
      901 gtttctgaat ggcggaggcc aggtgcggcg gcagcacgcc atcgtggcgg ttaacgagta
      961 acgtcgccag ccggggcgca tccagccgca cgtaaatgct gatccagccc ggatagttcg
     1021 ggttacattg gtcaacatcc gtctcttccg gccagccctg cgccagcagc aggttacggg
     1081 cgtgacgaca aatcagagca ttcagcgttt cattcataat tcatgaactc cctgtatcgc
     1141 cgcccgcagg cggcgataac atcagtcgtt ttcgtgtgtg ttgtttttca gcaggctgcg
     1201 ggcctgatca accatcgcct gtcggcgggt aacgtattcc gggaggggga aaccgtgatc
     1261 ccgggcagcc cattcacgtt tccacagtgc ctgttcagcg atctgagtca gggtctccag
     1321 cagttcagca gggacggtca cctggcggtt ttcgcgcacg ttgctgataa tctccatcgc
     1381 cgcggcggaa gaaataccgg actcaatccg ctagtcttcc gggatgtcct ccaggtgcgc
     1441 caggacggtc ctgatttcat gatcggacag ggaaatatcc attccgtcag cgagggcctg
     1501 aatctcttcc ggggaccaga taaccagcat cagcggcgtg tctcccgaat actttgctgc
     1561 cagcgcccgg catagcgttt cgcatgttcc gtacatactc tctcccctca atctttgaag
     1621 caggaatcca gttccagaat ttcggtatat gcctccatca agaggcggtt tgcgcgaaac
     1681 aacgtctgac gcaccagacg ttccggcagg gtgtcagcat taaagctgta ccgccgctcc
     1741 atctcattca tggttgtacg caggtcgcac atggcattgg ccacctccgc cacttcggcg
     1801 cgaagttttt cgtgtccggc atagttcatt tacgcagcct ccggcataaa ccagtcatca
     1861 ttcgcggccc cctgctgcat ggcccgctgt acggcagcca gtcgttgctg tccggcacgg
     1921 tgatactgct caagcagctc gataccgata taccggcgtc cggactggag ggcggcgacg
     1981 caggttgaac cgctgcctgc aaacgggtcc agcactatcg cattcgggtg tgtgaagctc
     2041 tcaatcagcg gttgcaggct ggtaacgggc ttttccgtcg ggtgatggcg gttgcccgaa
     2101 tatttccagc ccaacacgtc cggcagcggg ttttgtggca gacgtggacg gccttttgcc
     2161 aggatgtagg cgcattcgtg gcgatagccc acatatgcgg ccttcgatgt gtagtttttg
     2221 gtgaagacca ggtgaccaac aacgctgaat cccgcatttt tccaggcaga cataaagcga
     2281 tcgacgcggt tccagccata gaagctcacc atcagcgcgt cttttttgag tacgcggtac
     2341 atttcattac aggccggttg cagccattca tcagttttat caccagcaat ggtacgcccc
     2401 tgacggtcac gaaaaccgac gagatatggc ggatcggtga ggatgaaatc aatggcgtta
     2461 tcaggaatac gggccataac atcgatgcag ttaccgagga caaaacggga cataacagat
     2521 ccttctctta cagagggcac ccctgtggtg cccgttcccc gctgcctgtg ggctgttcat
     2581 cgcgtccggt aacgggtcag gccgaccgtg gaataccgca gccgtcgtct ttttgacagc
     2641 aaggttatgc cgcgaaagcg gcttgaaggg cgctcatcag aaaagggcga cgggctggcg
     2701 tacactgtca ccgacgccag ccgggagcgg taaaagcgcc cgtcccgtgc ccgccggacg
     2761 cgggacactg accacaggca gcgtttatgc cgcgccgcgt cagccggcgc agtaaaaaga
     2821 ggagcacggc gacatccttt gtcggaaaag aaacgggcgg ttgcccgccc gtctccgtca
     2881 tacttcatcc gccataatgc gccacattgt cgactatcca cgcaggtccg gtgacttccg
     2941 gctgctcatc gtcatctgtc ggggaagacc attcaagttc cccccacagc acatcgacga
     3001 gctctccacg ctcatacaac tgccagccac agaaatcaca gccctgttcg gcataccagt
     3061 gctccagcgt gcagctgaaa cggcggctta attctgcaat aacgtcgctt tccggctgac
     3121 accacggcgt gtcgaaatcc acctgaatga agttttctcc ctgactgctg atattcaggt
     3181 catatccaca aggccacttc acgccatacc gttccgtata ccagtgatat gcagaaggaa
     3241 caccgttcag aacaccacca ttaaagccgt tcacttcaac atccaggcgg gtcggcagaa
     3301 ccgtcagcag gtcaaacggc atggtttttt ccggcactac gttgtcacac agacggttcc
     3361 accacacaga tacatcctcg ttaccccata tgtcacacca gtcacctctt tttgcggtga
     3421 aatgcacagt gatggtttcc ctggcatcat ccggtaatcc ttcccagcga cgctggccag
     3481 taccgctctg taaccagagt tcatgcagca tcaggcagtt ttgttcatcc agcaacacac
     3541 cgttctgcag gtgtgtcagc cagcgggtga acgcgatatt ttccgtcgat acagcaccac
     3601 gtccggcagc cgtcactccg gggcactgtt cagagcggat attttcagtg atctgcagaa
     3661 gtccggcact tccggccaga aacagctgaa taccttcatt tgtggcgcgg cgataaagcg
     3721 gtgtgactgc accgctggcc agtcgtttaa tctcagcgat ctgtgccggt tcaccaggaa
     3781 aatacatacg attcgagcac cagtttggca tgggaattct cctctcacat gcggcctcca
     3841 tcaggccgtg ccgcacctgt aaccgggcga ccgcatacga atgcaggtgc ggccccgaag
     3901 ccgcagcgcg tggaggagga atgacgaaac gtgaccgggt gaggaggccg gcgacagcga
     3961 cacacttgca tcggatgcag cccggttaac gtgccggcac ggcctggtta agtagtatcc
     4021 gtctcagttc gtggcgcaaa atgagaaacc aataagaact caaaatgaac cacaacaaga
     4081 acataaagag actctaaaag agcaacaccg cttgacatag actcaaaatg agtcacaata
     4141 agaaacaata aaaaactcaa aaagatgcac cgatataaag gatgctcaat ttgagataaa
     4201 cacaaactca aaacgaggca aaaagaatgc taaaagtatc atgcgatgat ggttcaacta
     4261 acgtaaaact ggcctggctt gaagatggcg aggtaagaac cagtctgtca ggaaacagct
     4321 ttaaagaagg ctggaacccg ggactgttta atgccggcaa agtttacaat tacgtcgtgg
     4381 atgaaaaaaa atacacctat gatttgggaa gcactgctgt cataggtaca acacatgtca
     4441 gttatcaata cagcaccacc aatctgcttg ccatacatca tgcactgctg acatccggcc
     4501 ttcagccaca ggatgtagaa ctgacagtaa cattacctgt gaccgaattc tttgataatg
     4561 acaatcaacc aaatgaagaa agaatagaaa gaaaaaaggc aaatgttctc cgtgagatat
     4621 ctctgaataa aggggaaaca tttaaaatta aaaaagttaa tgtaatgcct gaatctttgc
     4681 cagctgcatt tgaatcacta aaaaaagata aagtaaacaa acttgaacgc agcctgatta
     4741 tcgatcttgg aggaaccact ctggattgtg gtttaattct cggtgcattt gaaggcattt
     4801 ctgaaatccg tggctattca gaaatcggga catcccgcat cactcatacc gtcatgaatg
     4861 cgctgacgaa agcctccaca ccatgtaatt attttattgc tgatgagtta ataaaaaaca
     4921 gacacgataa tgaatatctc cagacattaa taaatgatgt agctgaaata aaaaacattt
     4981 ctcatgtaat agacagagaa gtgaaatctc tggcagaaag catcaggcag gaaatttcaa
     5041 cattctcagg aatgaaccga atctatctca ctggcggagg agccgagctt atttaccctc
     5101 acataaagca atatttcccg aatctgaaag tgaacaaggt ggatgaacct cagtttgcat
     5161 tggtgaaagc aatggtgcat gcgtaatgga aagcagtgat ccaaaaaaaa gaaagaaagt
     5221 tgtggcatac ctgcacccgg cactttatcc gcaggacaac ctgacacaac aaacaataga
     5281 ctctctccca gtacaaatgc ggggagattt ctacagacaa tcccttattt gcggagcagc
     5341 tctttattct gttgaccccc gactgttaac gctaatcagc ggttttttca gtgagaaaat
     5401 cactgcagaa aatctggtga aacttataga acaaacaaca ggatacacat ccacatcaat
     5461 tgatataagt gtgttaaaaa acatcataga ggcatcttca gaaaacaaat cagagagcat
     5521 cacttcaaaa gatgatttcg aagaacaaac cagacgcaat ctttctatgt taaaaaaata
     5581 ataaaagaag agcaggttca gtcaattacc tgctcttctt ttatacatac taaaaaatca
     5641 gatttttacg aatattcaga ttatcataaa aaatgcaaca gacaggaatg caataatttt
     5701 gcattatctc gttgcttgta atgcaaatat gcattattgt cagtgtgtga caataacaga
     5761 gagatgacga tggcgaccat taacgcacgt attgatgatg atataaataa ccaagcagat
     5821 gaagtgctga agctgatgaa tatcagtcaa actcaggcta ttgcagcttt ttatcagtat
     5881 attactgaac aaaagaaact gccatttgtg atcacctcaa ttgtaaaaac gccgcatgat
     5941 ttattacgtg aaagtacaga catgttagct gaagctcttg cagtgatttc caatctacag
     6001 gtgtggactg aacagcagga tgggattgga aaagcaaagc taatggagta ttaccgccgc
     6061 ctcgacgcac tgtactgttg tgcaaaagag aaaataggtc ttctttcaga taaccgggat
     6121 gctgaactag gctgtgtccc ttaatcatct gagttatagt aactgtctgt ttacacaaca
     6181 tattgaacta tggctcgcta cgaccttccc gatgaggcat ggaccatcat caagcctctg
     6241 ttacctcctg aacccgccac accacgggcc ggacgcccat gggctgagca ccgtaaaatc
     6301 atcaatggca tgttctgggt gttatgttcc ggtgcaccat ggcgcgattt acccgaacga
     6361 tatggggcat ggaaaactgt ttataaccgc tttaaccgat ggtcaaagtc aggcgtgatt
     6421 aatattattt tcaacaggtt gctttcgcta cttgatgcaa acggctttat tgactggtct
     6481 gccaccgcgc tggatggcag taatatccgg gcgctgaaat gtgccgccgg tactcaaaaa
     6541 aacatcccga tatcgacgga gataatgggc cgggtcgctc tcgcggcggt tttggcacca
     6601 aaatccatct ggcaacagac ggaagtggcc tcccgttaaa tatcgtgcta agccccggac
     6661 aagctcatga aagccagttc gcgcaacgac ttctggacgg gattggcgtt cagcgtcaga
     6721 acggcagcat gaaacgccgt ggacatgcgg tactggctga taaagcgtat tcagggcgcg
     6781 cattgcgcaa tgagctgaaa aataacggta taaaggcagt aatcccccga aagtcaaatg
     6841 agaaaatggc atcggatgga cgtgcacagc ttgatcgtga tgcgtactgc aatcgtaatg
     6901 tcgttgaacg gtgctttggg cggctgaaag aatatcgccg catcgccacg cgttacgaca
     6961 aaacggcgag aaattacctg gcgatggtga aactgggct
//
LOCUS       DAJTYG010000149         6894 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTYG010000149 DAJTYG010000000
VERSION     DAJTYG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.149"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..90)
                     /locus_tag="OO568_003653"
     CDS             complement(<1..90)
                     /locus_tag="OO568_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HCR6642823.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRV"
     gene            146..298
                     /locus_tag="OO568_003654"
                     /pseudo
     CDS             146..298
                     /locus_tag="OO568_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1679..2626
                     /gene="icsP"
                     /locus_tag="OO568_003655"
     CDS             1679..2626
                     /gene="icsP"
                     /locus_tag="OO568_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR6642824.1"
                     /translation="MKSKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3188..3560)
                     /locus_tag="OO568_003656"
                     /pseudo
     CDS             complement(3188..3560)
                     /locus_tag="OO568_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3804..3923
                     /locus_tag="OO568_003657"
                     /pseudo
     CDS             3804..3923
                     /locus_tag="OO568_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4173..5039
                     /gene="ospB"
                     /locus_tag="OO568_003658"
     CDS             4173..5039
                     /gene="ospB"
                     /locus_tag="OO568_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR6642825.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5368..6108
                     /gene="phoN2"
                     /locus_tag="OO568_003659"
     CDS             5368..6108
                     /gene="phoN2"
                     /locus_tag="OO568_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR6642826.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(6461..>6894)
                     /gene="ospC4"
                     /locus_tag="OO568_003660"
     CDS             complement(6461..>6894)
                     /gene="ospC4"
                     /locus_tag="OO568_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR6642827.1"
                     /translation="KLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGDKKADI
                     ALYLLSKFNFTKQDIAEMEKMNNNIYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVN
                     KKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAVSGKRFER"
BASE COUNT         2163 a         1289 c         1335 g         2107 t
ORIGIN      
        1 aactctagga taaatatttc tcactatata ttcagtattt gcagtattta ttggaaatac
       61 gttcttaata ttcttgatgg gactaatcat tttaatctct atactcaaat ataaacatta
      121 ccatgaacaa tataattgtt cctaagtgga atgtctccac ggagaacact ttatctatcg
      181 ggaaatagtg cgggcaacag tgtttaatta tatcgagtgc gattacaatc gctggcgacg
      241 gcaccgttgg tgtggcggcc tcagtccgga acaatttgaa aaccagaacc tcgcttaggc
      301 ctgtgtccat attacgtggg taggctcaca gagcctcact tacgactgcg tccatattat
      361 gtgggtagga tcaataaaga cgttttatat gtgtctgctt gcagccagaa aatttatacc
      421 ctgcaggtat cataaacatg cttgataact taattggggc tccctcttcc ttttatggtg
      481 taattgtttg attgaatact tccggggatt tcagtatgaa atgaagtata tttaatatac
      541 tttacaaaaa gtttgtatta gtggaataag gacaatagct tatccaaact gccaagcctc
      601 tttattataa gtaagatctg gcactggaat attctcaaaa ggactaacat catcaccacg
      661 accttcaacc aaccatttta ttgcatttaa atcccagaat agttcagtag tggtcaattt
      721 gggaaacgca caatattgta cggttacgtt atcgcaatag agtgaactcc cgccctatac
      781 ggcgttttca aggattagtc cttaatcgga caaccacatt aaagcataat aaataattaa
      841 gctttaataa tatactatag atacagttct attataaaaa atactatttt aggtgatgca
      901 ttaaattagt agaatattta ttcttttata aaagcaccaa tgaattgtcc aagtaaacat
      961 gaggtaaaat gtgtagagga taactgagtt tggtcacttt aactgtatta gtcgcagtct
     1021 gactctcgac tttaaaagga tgggaattac ctgtactgat agtgagaaag ctttccattg
     1081 aaataagtca aataaaagtt gatggcttca gtgctaaagt gaggtgcccg ctagccgtat
     1141 tcactcgaat cggaattcaa tgtattgctt ctgccatttc cataatattt cacgggaaaa
     1201 acaaaatctg aaaaactata cttaagtgtt gtattgagtc agttgagata caaatacata
     1261 tttatcagca cacgttacgt caaatacagg aggttgtcat gttggtactg aaaggcacgt
     1321 tggctattgc agccgtgatg acaatttatt gtcttatcgt tgttcttatg gaacgctttt
     1381 ctgattgatt tcatattggc gagagaacaa gaaggtgagt agaattgtcg cggaatcctg
     1441 aaactatcag cctcaggtat gaacaccaaa aggtaggtag agaaatctat ggcccccgtt
     1501 actttattcg cacaatgagg tattttttta tttttacaaa agttcaataa atattataat
     1561 aaaagatatg ttcttggtaa cataaacaaa tatttaatta aaataatgct tggcattgac
     1621 gtttatctta taaagtaaga agatcatgga catttcaacc aaaaaagtag agttctcgat
     1681 gaaatcaaaa ttccttgtac ttgcactttg tgtacctgcg atctttacta cacatgctac
     1741 cactaactat ccacttttca taccggacaa catcagtacg gatattagtc taggatctct
     1801 gagtggcaaa acaaaagaac gcgtttatca tcccaaggaa ggggggagaa aaattagtca
     1861 actggactgg aaatacagta atgcgactat tgttagaggt ggcatcgatt ggaagctaat
     1921 tccaaaagtg tctttcggag tttccggttg gactacttta ggtaaccaga aagcaagcat
     1981 ggttgataaa gactggaaca attccaatac ccctcaggta tggaccgacc agagctggca
     2041 tcccaatacg catctccgtg atgctaacga attcgagctg aatcttaaag gttggttatt
     2101 aaataatttg gattatcgac ttggactaat agcaggttac caagagagtc gttacagttt
     2161 taatgcaatg ggagggagtt atatttatag tgaaaacggg gggaacagga ataaaaaagg
     2221 ggcacatcct agtggtgaaa gaacaatagg ttacaaacag ctcttcaaaa taccttatat
     2281 tggattaact gctaattacc gccatgagaa ttttgagttt ggagcagaac tgaaatatag
     2341 tggttgggtt ctttcatctg atacagataa acactatcag actgagacaa tttttaaaga
     2401 tgaaataaaa aaccaaaatt actgctctgt tgctgcgaat attggatact atgtcacccc
     2461 cagtgcaaaa ttttatatag aaggctccag aaattacatt tctaataaaa aaggtgatac
     2521 atctctttat gagcaaagta ccaatatatc tggcaccatt aaaaatagtg caagtattga
     2581 atatattggt tttctcactt ccgcaggtat aaagtatatt ttttgaattg gttcaccgag
     2641 ttgattacgt tttcatggct aaaaattgcc gcccattgtg agtcatattc ggcctgactt
     2701 tccagaacca tacgaactgc ccgttgacgg acttcggggg aaaaacgtgt atttttagtc
     2761 attctgttta cctctttctc agggcgttta gtctccagga tttccggggc ggttcaaaaa
     2821 actcttcgtt gattactgtg gacttactgt tggcattacc gaccctgaga ccggagaaat
     2881 aagaaccgct tagatcatcg tggctgttct cggggtatca agttacacat gggcagaggc
     2941 tacccaaggt ctactattat cgcctgaaag cgctgatgga acagtgctat cagggacatg
     3001 ctgatgaaaa tacctaaata atcagccagc actctatctt tccaaatcca cagcatagca
     3061 aagagagcaa aagagcctgt aaattcagaa attaaggaaa cgcctcttgg tgcacataat
     3121 ctatcttatg ttaaatgaaa tctaatttct tgattatatg ctggatcgtt cgtgggtggc
     3181 tttgccccta tatttccaga cacctgttat cattggcccg ctgccgcaga tattcccgtg
     3241 gcgagcgata atccagcgca ctatgcggat gccattcgtt ataatgctcg aatgcctctg
     3301 caaggttctt tgctgccgtt aacccgtcag gtttgggcat gatactgatg tagtcacact
     3361 ttattgtttt cacgaagctc tttgctattc tgttactctc cggactccgc accgccgtgt
     3421 tcttcggttc aacaaagtcc caacatccgg gtgaactggc gtgtttcatt agcccggtag
     3481 catgaaccat tatccgtcag tcactccact ggagacgacg gaagatcgtt gccgaagcgg
     3541 tgttccaccg ctcccagcat gtcgccaatg ggagagcatc tacggtctga ttgaactgtt
     3601 tattcaaatt gtccaccgtc tgtcagtacg ctctgaacgt cggctggtta aaacactgct
     3661 cgctgatttt cagagggttc acggtaaaac agcactttta ttccggattg cggaagcggc
     3721 tctgaataat ccggatgggc tggttaaaga ggttgtgtac catctgcata ttgttgaacc
     3781 agatcgcagc ggtaagagaa gttcatcata cttggcgcag ttacgcgatg tttctgctag
     3841 aggtgatgcc gtaaagaacg gcagaacatt accggaacaa gatagcggtc tacctgcact
     3901 ggtatcagac ccggggcttc cccggatgat ctccacggta ctttaggtac tgtgtctata
     3961 ttatgtaggt aggatcaatt aactttgtag ttgaaattct ttcattgaaa taatgaataa
     4021 aaaagacaaa ccgcattaaa caatgccaga atataattgc atttttgttc cgttttttta
     4081 aatcatagta tacaaagaag cttgcatact atatgaaaat ataacaccct ttactatgat
     4141 gtttataaaa caatatatgg agtcatgtag gtatgaattt agatggtgtt agaccatact
     4201 gtagaatagt caataaaaag aatgaaagca tatcagatat tgcatttgca catataataa
     4261 aaagggtaaa aaattcatca tgtactcacc caaaagcagc attggttttt ttaggagaga
     4321 aaggtttttg tgatagcaat gatgttctat ctattatggg acaacaaata ccaagagtat
     4381 ttaagaacaa gatgttatat gattatgttt ttaaaaatga aaaaagtaaa aatgattttc
     4441 taaaaatggc tgaatcatgg ctaccacaga gtgaaccaat agtaataaat aatgatgatg
     4501 acgcattgaa tgctgctgct tatttttctg taaaaaaagc gaaaataaaa acagtaaacg
     4561 atactgattt taaagagtat aataaggttt atattcttgg gcacggtagt cctggttctc
     4621 atcaattagg ccttggttcg gaacttattg atgtacaaac aatcatttca agaatgaaag
     4681 actgtggtat tctaaatgtg aaagatatcc gttttacttc atgcggctcc gctgataaag
     4741 tggctcctaa aaattttaac aatgcccctg ctgaaagtct ttcttgtatc cttaactctc
     4801 tgcctttttt taaggaaaaa gaatctttgc tagagcagat aaaaaaacac cttgaaaacg
     4861 atgagtcatt gagtgatggt ctaaaaatat ccggctatca tggatatgga gttcactatg
     4921 gtcaagagct ttttccctac tcacattatc gttcaacttc aattcctgct gatccggagc
     4981 atacagtaaa aagaagctct cagaaaaaga cttttattat taataaagaa ctggattagt
     5041 ataaaatttt taacctatag ttaattgttt gcatttaaga tgaagttatt ttatctttag
     5101 tagaaaagtg cttccctatg agggaaaatc acaaatcatc ataatacaga gacaaaacga
     5161 tacgaacaaa tagataaaaa acatcattct tttaccacat tatttttcgt gaatatgaaa
     5221 aataatgtta ttactttaat ataagactat tttttgtttt tccatcactc tgttcaaatt
     5281 tttccgcatg acttgtgttt tttgtaacac agctcgtttt ttacagctga ccaaaatcat
     5341 caattaatta tgctaaggaa ataaattatg aaaaccaaaa actttcttct tttttgtatt
     5401 gctacaaata tgatttttat cccctcagca aatgctctga aggcagaagg ttttctcact
     5461 caacaaactt caccagacag tttgtcaata cttccgccgc ctccggcaga ggattcagta
     5521 gtatttcagg ctgacaaagc tcattatgaa ttcggccgct cgctccggga tgctaatcgt
     5581 gtacgtctcg ctagcgaaga tgcatactac gagaattttg gtcttgcatt ttcagatgct
     5641 tatggcatgg atatttcaag ggaaaatacc ccaatcttat atcagttgtt aacacaagta
     5701 ctacaggata gccatgatta cgccgtgcgt aacgccaaag aatattataa aagagttcgt
     5761 ccattcgtta tttataaaga cgcaacctgt acacctgata aagatgagaa aatggctatc
     5821 actggctctt atccctctgg tcatgcatcc tttggttggg cagtagcact gatacttgcg
     5881 gagattaatc ctcaacgtaa agcggaaata cttcgacgtg gatatgagtt tggagaaagt
     5941 cgggtcatct gcggtgcgca ttggcaaagc gatgtagagg ctgggcgttt aatgggagca
     6001 tcggttgttg cagtacttca taatacacct gaatttacca aaagccttag cgaagccaaa
     6061 aaagagtttg aagaattaaa tactcctacc aatgaactga ccccataaag ctggacagcc
     6121 tgtatcaggc tatggagggc ccatagacaa atctacccta tatgagcata ggaggagtct
     6181 atgggcacac cacgttttac ccctgaattt aagggattac tggaaaggct gggacatatc
     6241 cctccggcag aagcagaaaa agcttattat gctgccatcg gaaacgatga tctggcaacc
     6301 tgagttcaca gataaaacat tctctaggaa actcggggcg gttccgttca ccacatgcaa
     6361 tgtggtgttg caggggaacg gtctgcacca tcccctatat cgatcaatat aacaaaaatg
     6421 acaacttcag attcagggca caacctaaat atattttagg tcacctctca aatcgtttgc
     6481 ctgataccgc tccatttttt aataaaaaat caatcatttt tgaatctttg cttttcattg
     6541 cattgtcaag catagtgtcc ccactatttg ctttttgaaa ttttttgttt acatcaacca
     6601 atccattatt gataaaatat tctagcactt tatagttggc accatcttta ctaagtaagt
     6661 attcaacatc atacagatta caatatatat tgttgttcat tttctccatc tctgcgatat
     6721 cttgttttgt aaaattaaat ttagatagta aataaagtgc tatatcagct tttttatcgc
     6781 ctaaagaata agcaagggcc tgatgagcca tttttttatc agtcacttta ttgttaatct
     6841 cttcataatt agatgcttta atcgcatcct ccaaagatat gtctctgagt ttta
//
LOCUS       DAJTYG010000150         6884 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTYG010000150 DAJTYG010000000
VERSION     DAJTYG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.150"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            57..1328
                     /locus_tag="OO568_003661"
                     /pseudo
     CDS             57..1328
                     /locus_tag="OO568_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804911.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            1341..1787
                     /locus_tag="OO568_003662"
     CDS             1341..1787
                     /locus_tag="OO568_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR6642828.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIFIRHV"
     gene            1889..3412
                     /locus_tag="OO568_003663"
     CDS             1889..3412
                     /locus_tag="OO568_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6642829.1"
                     /translation="MKRVISYIRFSSERQAKGDSLRRQSKMVNDWLKAHPEYYLDQDL
                     SFRDLGVSGFTGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDVDQASELLR
                     NILRSGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRSHEESLRKSERIQAAWNRKK
                     ELISEGIKISKRCPAWLKLNDDRKSFSVIPEKVETVKRCFQLRLEGKSIWEIVRTLND
                     EGHQTLNQYKAGSFGQTSVQDLLKNRSVIGYFTPKGCEEIADYYPAIIDETTFYKVQQ
                     LSYARYGRKPASDKPMAVNLFKGVIRCSECGHALIITGFSDTRNGIYRCPLRNENRCS
                     AKGLSRKQTDGTLLTLLSRLDRFDSDTTNTIDTLKLHREHISSNIEKLVQLSLEIEDL
                     SAITSKLKQLKNELSQVDADIEKETQRLKSIDVGCLSDCDLTTKAGRIEAQLIIKSAV
                     KEVVLNTAKRRCKVTFHNGKVIDLPITENPSDNVTEAIQSLSEVTERGLIDVDEVII"
     gene            complement(3427..4200)
                     /locus_tag="OO568_003664"
     CDS             complement(3427..4200)
                     /locus_tag="OO568_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642830.1"
                     /translation="MIYTSQLRYTGKDGVYFHLQGQPDNFLTLSATEQMEYDSPMMVT
                     TQNMQSGQTVTDNVMRSPRTISISGVVVVGESGSILISRDNKLVENFIDTLENWRDQK
                     QIISVICKDGIKIDDSIITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDISAATG
                     KTATTNDGGATSKKNTGNTTTSLGNGMLMCQELYNRSTSELSNEQLQARINCGRNVRV
                     KNGKSTFSDAANEQASKVLNNAGTGSTLKKFSVNPNKRY"
     gene            complement(4364..6454)
                     /locus_tag="OO568_003665"
     CDS             complement(4364..6454)
                     /locus_tag="OO568_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6642831.1"
                     /translation="MSKFTVDSFIVELGFNENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQ
                     PLISGNRAITAAYSANMSKLKGFDPILQKYIKSQFYGLSAKAGSMDSSKFNEKLAQLN
                     ASVREAIAKARGHTSTSINADRAANSLDNLSSAAVKTAGAFISMQAVLASYQKIMEIG
                     LQRASAERSIDFVFDKDAGQVKEFTKSLAQTTGLDIAELQSQFAGFGASAKESMGIQG
                     SEELFKNMIGYARLMGRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVF
                     AKATGKSEQELFDAMKKGALKSADTLQKVTQELNRQITAKGGWKAISESTQAQLGNLK
                     NSWNTTLDSIFRGSENGLQDFTRSLTTLLNALGGSGKSLGESLGSLMSDMSHGVDSLT
                     DISYRVRAFFDEVTLAYRGLTDEQKKVVDALGSGLINSLKVLAGVIAVNKVVGVTRSI
                     YGLMTAITRLGQVADTTADNVNRKNGKPSRLNPMNMLLAGSAIYAGYERLASTPERME
                     AQAKLPELAKESGLKPVFTMDDVKGWFSSAADKIANSQEPMNNFVNNTQSTPPAITGD
                     MVNSNAQLAMTISELSRKVDALTVEAKKPLSVNITGEAIVRPDEPAFFTFNQSANDQF
                     TQDMLLSSSYPEEE"
     gene            complement(6663..6866)
                     /locus_tag="OO568_003666"
     CDS             complement(6663..6866)
                     /locus_tag="OO568_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642832.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSSTSKQKNRIRRIAK
                     WCLDKLPSDCQIVGEDGNYYLVR"
BASE COUNT         1956 a         1484 c         1415 g         2029 t
ORIGIN      
        1 atttaatatc atgaaatatg taagctcgta tcgctcttgg gcgtctggag gtatcgatgg
       61 ataaagtcaa atatccaaca ggcgtcgaaa accacggcgg cacattacgc atctggttta
      121 attttaaagg taaacgtgtc agggaaaatc ttggtgtccc tgacactgcc aagaacagga
      181 agatcgccgg ggaactgcgg acatcggtat gttttgccat ccgcacagga agctttgatt
      241 atgctgcaca gttccctgac tcccctaacc ttcaggcttt tggggtaagt aaaaaagaaa
      301 ttacggtgaa ggaacttgaa gaaaagtggc tggatctgaa acgaatggaa atctctgcaa
      361 atgcattcaa tcgctatgaa tccgttgcaa gaacgatggt tccgaaaatt ggaggcggca
      421 gactggtgtc atcggtaacc aaagaggaat tgctgtatat caggaaagat ttgctgaccg
      481 ggtatcagaa ttcaacgaaa aacaaagcag cagcaaaagg acggagcgtc gttactgtaa
      541 attattacat gacgacaatc gctggaatgt ttcagtttgc tgcagatcac ggttacttag
      601 aagcaaatcc cttccaggga attaagcctc ttaaaagagc cagggcagag ccagatccgt
      661 taactcgtga cgaatttatt cgcctgatag atgcttgccg acatcagcag acgaaaaacc
      721 tgtggtcatt ggctgtgtac acaggaatgc gtcacggtga actggtctcc ctggcctggg
      781 aagatatcga tatgaaagca ggaactatta ctatcaggcg caattatacg aaactcggtg
      841 agttcactct acctaaaact gaagcaagta caaacagggt tgtgcatctt atccagcccg
      901 ctatcagtgt cctgaaaaat caggctgaaa tgacaagact gggtaagcag caccacatca
      961 aggttcaact acgtgaatat ggacgttcag tgaatcatga atgtactttc gtatttaacc
     1021 cccaggtggt tagaaaaagc aaacaggtag gttttatcta caaggtagat tccattggcg
     1081 actcatggga aacagccatt aagcgtgcgg gcatcaggca ccggaaagca taccagtcac
     1141 gacacactta tgcgtgctgg tcattatctg ccggagcaaa cccaagcttc attgccagct
     1201 agatgggcca tgcaagtgcc cagatggtgt tcaatgtata cggagcatgg atgactgaca
     1261 gtaatgcaga acagatcgca atgctgaatc agaagctggc agattatgtc ccatggtcac
     1321 caaagtgaca caagagactt attaaaatca gtgggttagc cttcaatacc cgtcatgtta
     1381 actgcgtgga gggcaacacc acgctttacg ccctgccgaa acccgaggtt gtcctgcgct
     1441 ggcgtgaaca gaccacagat gacttccgct tctgttttaa gtttccggcg accatttcgc
     1501 atcaggcagc attacgacat tgcgatgatt tagtgactga atttttgacc cgcatgtcac
     1561 cgttggctcc gcgcattggg caatactggc tgcaactgcc tgccacattc ggcccacggg
     1621 agctgcctgc gctttggcat tttctcgatt ctctccctgg cgaatttaat tatggtgtgg
     1681 aagtccgcca tccacagttt ttcgccaaag gggaagagga acaaacgctt aatcgcggtt
     1741 tacatcagcg cggcgttaat cgggtgattt tcatcagaca cgtttaaata caccacctaa
     1801 agcgatacaa tctaggggtt ggtagatgat tcggatgaag catcctcata acaagcccaa
     1861 ctgctattag tttttatggt ggtaaagaat gaaacgtgta atcagctaca tcagattcag
     1921 ttctgagcga caagccaaag gcgactcact acgcagacag tctaagatgg ttaatgactg
     1981 gcttaaggct catcctgagt actaccttga ccaagattta tccttccgtg atttgggggt
     2041 gagtgggttc actggtaagc acttgaaagg tggcttaggt gactttttaa ccgctatcga
     2101 gaaagggctt gttaaagctg gtgatacgct cttgatagaa tctcttgacc gtctgtcacg
     2161 tcaagatgta gaccaagcaa gtgagctact cagaaacatt cttcgctctg gtgtagatgt
     2221 ggtcaccttg tccgatggtg aacattacac acgagaatct cttaaagacc cgttagctct
     2281 gattaaaagc attctgataa tgcagcggtc gcatgaggag agtttgagga agtctgagcg
     2341 tatacaagct gcatggaacc gtaaaaaaga actcatctct gaaggtatca agatttctaa
     2401 acgttgtcct gcatggctta agcttaatga tgaccgtaaa agcttctctg taatccctga
     2461 gaaagtggag actgtgaaga ggtgctttca gctacggtta gagggtaagt ctatctggga
     2521 gattgtcagg actcttaatg atgaaggtca ccagacgctt aaccaatata aagcgggttc
     2581 atttggtcaa acctcagtac aagaccttct gaaaaatcgc tctgtaattg gttacttcac
     2641 acctaaaggc tgtgaagaga tagctgatta ttatcctgca attatagatg aaacaacatt
     2701 ctacaaagtc cagcaactat catatgctcg ttacggtaga aaacctgctt cagataagcc
     2761 aatggctgtg aatcttttca aaggtgttat ccgttgctct gaatgtggtc atgctctgat
     2821 tatcacaggc tttagcgata caagaaacgg gatttatcgc tgtccccttc gtaatgaaaa
     2881 ccgttgtagt gctaaagggc tgtcacgtaa gcaaaccgat ggtacattgc ttaccctgct
     2941 atctcgtctt gaccgatttg atagcgatac gacgaatacc atcgatacac tgaagttaca
     3001 ccgtgaacac atctcaagca atattgagaa gctggtacag ctatcacttg aaattgaaga
     3061 tttgtcagct attaccagca agctaaaaca actaaagaat gagctatcac aggttgatgc
     3121 ggatatcgag aaggagacac aacgccttaa atctattgat gtggggtgtt tgtctgattg
     3181 tgacttgact acaaaggcgg gaaggattga agcacaactg ataatcaaaa gtgctgttaa
     3241 agaggtcgtt ttaaatactg ctaaacgtcg atgtaaagtg acttttcaca acggtaaggt
     3301 aattgaccta ccaataacag aaaacccctc agacaacgtt acagaggcta tacagtcgct
     3361 ttctgaagtc actgagaggg gtttaataga tgttgatgag gttatcatat agttgagatt
     3421 ctatcgtcag tagcgcttat tcggattcac cgaaaacttc ttcaaagtac tacctgtacc
     3481 agcattatta agcacctttg aagcttgctc attagcggca tctgagaagg tgctttttcc
     3541 gttcttaact ctgacattcc taccacaatt gatacgggct tgaagttgct cattagaaag
     3601 ttcactcgta gagcgattat atagctcctg acacatcaac ataccgttac ctaagcttgt
     3661 cgtagtgttc cctgtattct ttttgctggt agcaccacca tcatttgtag tagctgtctt
     3721 tccagtagca gccgaaatat cagtttgacc cacaatagct ttaaagttaa tttcctgaaa
     3781 agttagctgg attcttagac catttgaaat accaacatct ttagaggctt taaagcttgt
     3841 aataatggag tcatcaattt taataccatc tttacagatg accgaaataa tctgcttctg
     3901 gtcacgccag ttttcaagag tgtcgatgaa gttctccacc agcttattat ctcttgagat
     3961 aaggatacta ccggattcac caaccacaac cacaccactg attgaaatag ttcttggtga
     4021 acgcattaca ttatctgtta cagtctgccc tgattgcata ttttgagtgg tcaccatcat
     4081 agggctgtca tattccatct gttcagttgc tgataaggtt aagaaattat ctggctgtcc
     4141 ttgtagatgg aagtaaacac catctttacc agtgtaacga agctgactag tgtaaatcat
     4201 taaattctcc tttgatttca gagacttagt gtattttaat catttttaac caagcctttg
     4261 atatataagt gttttctctg ttaagcgtga gctacgtaaa aaataatttt tggtgggtat
     4321 atctgttcag aagatttcac gtagcacacg gatatataag aaatcattct tcctctggat
     4381 agcttgaact taagagcata tcctgcgtga actggtcatt agctgattga ttgaaagtaa
     4441 agaaagctgg ttcatccggt ctaacaattg cttcaccagt gatattaact gacaagggct
     4501 tcttagcttc tactgttaaa gcatcaacct tgcgtgatag ctcactaata gtcattgcaa
     4561 gttgagcatt actattaacc atgtcacccg taatagctgg tggagttgat tgtgtgttat
     4621 ttacgaagtt attcatcggt tcttgggagt tagcaatttt atcagctgca ctactaaacc
     4681 aacctttaac atcgtccata gtgaacactg gtttaagtcc agactcttta gctagctctg
     4741 gtaatttagc ttgtgcttcc attcgttcag gtgtagaagc taatctttca taacctgcat
     4801 agatagcact accagctaat agcatattca tagggtttag tcttgatggt ttcccattct
     4861 ttctattcac gttatccgct gtggtatcag cgacctgacc taatcttgtg atagcagtca
     4921 taagcccgta aatacttctg gttacaccaa caactttatt cacagcaata acaccagcca
     4981 agacttttaa actattaatc aagccagaac ctaaagcatc aactactttc ttttgctcat
     5041 cggttaaacc acgataagct aatgtcacct catcgaagaa tgctcttacc ctgtagctga
     5101 tatcagtcaa tgaatcgaca ccatgcgaca tatcgctcat caagctacca agagattcac
     5161 caagactttt accacttcca ccaagagcgt ttaaaagcgt tgttaggctt cttgtgaaat
     5221 cttgaagacc attttcagaa cctctgaaga tactatcaag cgtggtattc caagagtttt
     5281 tcaagttccc tagttgtgcc tgtgtacttt ctgaaatggc tttccagcca cctttagcgg
     5341 tgatttgtct attcagttcc tgagtgacct tctgaagagt atcggcagat tttaaagccc
     5401 ccttcttcat ggcatcaaat aattcctgtt cactctttcc ggttgcttta gcgaatacct
     5461 gaaccatacc aggaacagct tcagccagtt gccccttaag ctcttcagcc ataatctgac
     5521 ccttaccagc catctgtgat aatgctgtca gtgctcgctt aatctcttct tcacttctcc
     5581 ccatcagacg agcatagcca atcatatttt taaaaagttc ttcagaacct tgaataccca
     5641 tagactcttt agctgaagca ccaaaaccag caaactgtga ttgcagttca gcgatatcta
     5701 aacccgttgt ttgagctaat gactttgtga actcttttac ctgacctgca tctttatcaa
     5761 atacgaaatc aattgaacgc tcagcacttg cacgctgtaa accaatttcc ataatcttct
     5821 gataactggc taatacagcc tgcattgaga taaatgcacc tgctgtttta actgctgctg
     5881 aagacaggtt atcaagacta ttagctgctc tgtcagcatt aatgctggtg gatgtatgac
     5941 ctctggcttt agcaattgct tctctgactg aagcgtttaa ctgagctaac ttctcattga
     6001 attttgaaga atccattgac ccggctttag cagatagccc gtaaaactga gatttgatgt
     6061 atttctgcaa aatagggtca aagcctttta gcttagacat attagctgag taagccgctg
     6121 tgattgctct gttgccgcta attagtggct gtcttggctg tctaagtggc tttaaagtat
     6181 cctcaatttc cgttttaatc ttaaacgctc tagtgttttt aagacgctgc tcaatcttgt
     6241 caaaggtctt attaaacttc ctttcaagtt gccctaaaga acttgtcaga ttgctgtcaa
     6301 gttgcttggt atcgacctta aacgctctat tcaaattgcg ttcaattctt tgtgctgact
     6361 gcaaagctgc cttttcaact ctttgcagtc ccttaataac gttttcattg aagcctagct
     6421 caacgataaa actatcaact gtgaatttgc tcattcatat ttttcacctc tttaagcgtg
     6481 ttgtacgtaa attctatttt tcagtgtggg tattagcttt acagatttca tgaggataat
     6541 tcttatccga atcatcacgt agcacacagg attcgataga agactatctc atatacaaaa
     6601 aagggagagc taccgatgtc actcgataat ctctcccgtt gatttttacc caaccaatct
     6661 ttttacctaa ccagataata attaccatct tcaccgacaa tctgacaatc acttggtaat
     6721 ttatcaagac accatttagc gattcttctg attctattct tttgcttaga ggtggatgat
     6781 ttgattcgtt ttggtttgtt gggttgttct ttcaatcgtt cactaattgc tttaataagc
     6841 tcatagcgtt ctgttaaatc aatcattcac cacctccttt taat
//
LOCUS       DAJTYG010000151         6814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTYG010000151 DAJTYG010000000
VERSION     DAJTYG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.151"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..980
                     /locus_tag="OO568_003667"
     CDS             <1..980
                     /locus_tag="OO568_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6642833.1"
                     /translation="KYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGS
                     GLGIAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGR
                     TVLSSIYQDQRTKQHVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFL
                     NVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFW
                     ILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVM
                     FIAIDMDKLKQINDNLGHQEGDLAITLHDAYKASDERLYVNKQNKNSRS"
     gene            1023..1526
                     /gene="yeaK"
                     /locus_tag="OO568_003668"
     CDS             1023..1526
                     /gene="yeaK"
                     /locus_tag="OO568_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR6642834.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNTGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1527..1631)
                     /locus_tag="OO568_003669"
     CDS             complement(1527..1631)
                     /locus_tag="OO568_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642835.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1801..2247
                     /locus_tag="OO568_003670"
     CDS             1801..2247
                     /locus_tag="OO568_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR6642836.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2204..3025)
                     /gene="nimR"
                     /locus_tag="OO568_003671"
     CDS             complement(2204..3025)
                     /gene="nimR"
                     /locus_tag="OO568_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR6642837.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3122..4303
                     /gene="nimT"
                     /locus_tag="OO568_003672"
                     /pseudo
     CDS             3122..4303
                     /gene="nimT"
                     /locus_tag="OO568_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4358..4705
                     /locus_tag="OO568_003673"
     CDS             4358..4705
                     /locus_tag="OO568_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR6642838.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4727..4981)
                     /gene="yoaF"
                     /locus_tag="OO568_003674"
     CDS             complement(4727..4981)
                     /gene="yoaF"
                     /locus_tag="OO568_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR6642839.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5164..6189
                     /gene="dgcP"
                     /locus_tag="OO568_003675"
     CDS             5164..6189
                     /gene="dgcP"
                     /locus_tag="OO568_003675"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR6642840.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6222..6320
                     /locus_tag="OO568_003676"
     CDS             6222..6320
                     /locus_tag="OO568_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR6642841.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6323..6397
                     /gene="yoaJ"
                     /locus_tag="OO568_003677"
     CDS             6323..6397
                     /gene="yoaJ"
                     /locus_tag="OO568_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR6642842.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6449..6811)
                     /locus_tag="OO568_003678"
     CDS             complement(6449..6811)
                     /locus_tag="OO568_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642843.1"
                     /translation="MRRNLEKLAANHPELRRKRTGSKVLEFHISVLPAAARVELLLKS
                     EQIETSQGYFEIARPTLEAHDYDREALWSKWDKASDSQRRLAEKWLPAVQTADEMLQP
                     LLVRLSCYLSTGRLKVNA"
BASE COUNT         1649 a         1649 c         1661 g         1855 t
ORIGIN      
        1 ataaataccc gggcttacgc ggcacactac aaaccgcatc cactgactgc gacacaattg
       61 tggaagctgc agcactatta cccgcttttg atcaggcagt ggaaggcaac cgccaccagg
      121 atgattacgg ttcaggtctt gggatagccg aagagaaatt tcactattat ctcgatctca
      181 atgaccgcta tgtctatttt tatgagccgg ttaatgttga atactttgcg atgaataact
      241 ggtccttcct gcagtcagga agtattggca tcgatcgcaa agatattgaa aaggtattta
      301 ccgggcgtac cgtattgtcg agcatttacc aggatcagcg tactaaacag cacgtgatga
      361 gtttgctgac gccggtatat gtcgcagggc agctaaaagg gattgtgctg ctggatatta
      421 acaaaaacaa tttgcggaat atcttttaca ctcatgaccg ccctctcctc tggcgttttc
      481 tcaatgtcac gctaaccgat accgattcgg ggcgcgacat tatcatcaac cagagcgaag
      541 ataatctgtt ccagtatgtc agttacgtcc atgacttacc gggcggcatt cgtgtctcgt
      601 tatccattga tattctttac tttatcacgt cttcgtggaa aagcgttctg ttctggattt
      661 tgacggcgtt aattttgctg aatatggtcc ggatgcactt ccgtttatac caaaatgtgt
      721 cgcgagaaaa tattagtgat gcgatgactg gactgtataa tcgcaaaatt ttaacccctg
      781 aactggagca gcggttgcag aaactggtgc aatccggttc ttcggtgatg tttattgcaa
      841 ttgacatgga caagttaaag caaataaatg acaacctcgg tcatcaggag ggggatttag
      901 cgattacgtt acatgatgcg tataaagctt ccgatgagcg tttatatgtc aataagcaga
      961 acaaaaacag ccgttcatga taaccttctg tggttgtttg cttgtaatct caggagcgtg
     1021 aaatgactga aatggctaaa ggaagcgtga cgcatcagca gttaatcgct ttattatcac
     1081 aagaaggtgc taacttccgc gtcgttaccc atgaagcggt aggtaaatgt gaagcggtgt
     1141 cagaaattcg tggcaccgct ctaggccagg gtgctaaggc actggtatgt aaagtcaaag
     1201 gcaatggcgt aaatcaacat gttctggcga tcctcgcagc cgatcaacag gccgatctga
     1261 gccaactcgc cagccatata ggcggattac gcgcttcgtt ggccagcccg gcggaagttg
     1321 atgaactcac aggctgtgtc ttcggcgcga tccccccttt cagcttccat ccaaaactca
     1381 aactggttgc cgaccctcta ctcttcgaac gatttgatga aatcgccttc aatacaggga
     1441 tgctggataa atccgttatt ttgaaaaccg cggattattt gcgcattgcg caaccagaac
     1501 tggtcaattt ccgccgcact gcgtagctag cctatccgtt caataagcag aacggacaaa
     1561 accagcacaa ccgcaatagc aaaaaacgat gatgtgataa tcagtgtctc gacgaacatt
     1621 tgatcgttca tagcatgccc ccgggagata gctgttttca cgttattgtt agcgtgcaca
     1681 aatggcagtt tgatgacagt tcgcctgatt ttttatgcaa aaaacgtaaa gattttctac
     1741 ttccttcctg cagcaagcgt aaagtaagca ggcttattat tttttggcaa ggaaaccacg
     1801 atgtttgatg tcactttgct gatcctgctc gggttagctg cgctgggctt tatcagtcat
     1861 aacaccactg tcgccgtttc aattctggtg ttgatcattg tccgcgtcac accgttaagc
     1921 acctttttcc cgtggattga aaaacagggg ctaagtatcg ggataatcat tctgacgatt
     1981 ggcgttatgg cccccatcgc cagcgggacg ctaccgccct caaccttgat tcactcgttt
     2041 ctgaactgga aatcgctggt ggcaattgca gtaggcgtta ttgtctcctg gctgggtggg
     2101 cgcggcgtga cgttaatggg cagccagccg caactggttg ctgggctgct ggtgggcact
     2161 gttttaggcg tagcgctgtt tcgcggcgta ccggttggcc cgcttattgc cgcgggtctg
     2221 gtttcgctga ttgtggggaa acagtagtta atcccgcaat atatcggcct ggcgtttgcc
     2281 ctaggccttt cttaaacata gtaataaagg ccgttgttga atcatacccc agcgtatgag
     2341 caactttctg tacagtatcg cctttcacta atccctgaag cgccataatc agctgtaact
     2401 gctgacgcca ctggcgaaaa ctcaaccccg tctctttcac tatcagtcgg gcaagattgc
     2461 gttcactcat cgcaaaaaag ccagcccatt gccccaatga cccccactcg acgggccctt
     2521 tcgccatcat ctccaccatc gtgcggattt taggatgaga agaaacgggc aggtgcagct
     2581 gttgttgtgg ctgctgaggg agttcatcaa acaggacctg aattaatctg cgggtcatcg
     2641 gctcagcacg ctgagtcgtc gtcctgtttg ccagggttaa aattaattcc cggcataagg
     2701 gcgatatttt caatgtgcaa catgttgtcg gcattgtcac tgcgctgggt tcgataaaca
     2761 gaaaacagag ttcagcgtta gctgtcactt gattgctatg cttcaccccg cccggtatcc
     2821 aaaccgcata ttgtggcggc accatccaca aagcattttc caccgtacag gtaattgcac
     2881 catgtaatgc tagtatcagc tgtcctttgc ggtgctgatg tacaggactg gttaactcat
     2941 ccgttccggc gtgaatacaa aatgcgaccg cggcttcatg gtgtcggtcg ggttcatagc
     3001 cattgagatt caacctgtgc atcattttgt ccgaacttag cgataatttg tcattttagc
     3061 ttgattcaac ataacaataa aaacggtaag gtacagcctc gtttgtaaca atgagaagca
     3121 tatgacctgt tcaacttcat taagcggcaa aaacaggatt gtccttatcg ctggcattct
     3181 gatgattgcc acaacattac gcgtcacctt taccggcgca gcgccgttac tggatacgat
     3241 tcgttccgct tactcgctga cgacagcgca aaccggctta ttaaccaccc tgccattatt
     3301 ggcctttgcg ctaatctcac ctctggctgc cccggtggcg cgacgttttg gtatggaacg
     3361 tagcctgttt gccgcgttac ttttgatctg tgcaggtatc gcaattcgtt ctctcccttc
     3421 gccttactta ttatttggcg gtacagcggt cattggcggt gggattgcat taggcaatgt
     3481 cttactgcca ggattaatta aacgcgattt ccctcattcc gtcgccagac ttaccggcgc
     3541 atattccctg acaatgggag ctgcagcggc actgggatcg gctatggtcg tgccgctggc
     3601 tttgaacggt tttggctggc aaggcgcgtt gttcatgctg atgtgttttc ctctgctggc
     3661 tcttttttta tggctgccac agtggcgaag tcagcaacat gcaaatttga gtacctcacg
     3721 cgccttacat actcggggta tctggcgttc gccacttgcc tagcaggtaa cattgtttct
     3781 tgggatcaac tcactggtct attacgtgat tattggctgg cttccggcga tcctcatcag
     3841 tcacggctat agcgaagcac aggcgggttc actgcatggt ttgctgcaac tagccacagc
     3901 agctcccggt ttgctgatcc cacttttctt acatcatgtg aaagaccagc gtggtattgc
     3961 agcgttcgtt gccttgatgt gcgcagtggg cgcggttggg ctctgcttta tgccagcgca
     4021 cgcgatcacc tggactctgc ttttcggttt tggttccggc gcaacaatga tactggggtt
     4081 gacgttcatt ggtcttcggg caagttctgc gcatcaggcg gcggcactct cggggatggc
     4141 acaatccgtc gggtatttgc tggcagcctg tgggccgccg ctaatgggta aaatacacga
     4201 tgctaacggt aactggtccg taccactgat gggtgttgca atactttcac tactgatggc
     4261 gattttcgga ctttgcgccg ggagagacaa agaaattcgc taatatccgg tgctatagtg
     4321 acgtgacaaa tcttgcgtga aagggagaac aaacacgatg aatattcagt gcaaacgcgt
     4381 ttatgatccg gctgaacaga gcgatggtta tcgcgtactg gtcgaccgcc tctggccgcg
     4441 cggtatcaaa aaaaccgatt tagcccttga tgagtgggat aaagaaatca cgccgtcaac
     4501 ggaactgcgc aaagcctttc acggcgaagt cgtcgatttt gcaacctttc gcgagcaata
     4561 tcgcgcagaa ctggcgcaac acgagcaaga aggaaagcgg ctggcggaca tcgccaaaaa
     4621 acagccgctg accctgctct acgcagcaaa aaacaccacg cagaaccatg cgctggtgct
     4681 ggccgactgg ctacgtagct tgtgatttta gtacggcatc cggcggttat ttttcaccag
     4741 ccggatggtc ccgtcgccac aatgcccatt catcaatcgt ttcaccgccc ggtaatttgc
     4801 aattgttgct gaccccttgc gctgtctgca ctggaatgag cgtcccgccc ttctgctggc
     4861 aatagaccga cgccggattt gccataccaa tctgcggcgg tttaggtgct tctggctgag
     4921 aaggggttga acaaccagcc aggaccagca agcaaggcag aacaaaactg ataattttca
     4981 tttattgatc tcacatattt atccaagctt agagtatcgc ggtatcgttt tgttttgcag
     5041 cactattttt attacattca ctcaaaacat attacgtctt gtttcatctt tgttgatgat
     5101 gttttatcat gcctgcaaag attaaataat cagcacttac ccgccgtatc ctggagttgt
     5161 tccgtgtcag atcagattat cgcccgcgtc tcgcaatccc ttgccaaaga acagtcactg
     5221 gaaagtctgg tccgacagct tctggagatg ctggaaatgg tcactgatat ggaatcaacc
     5281 tacctgacca aagtggatgt cgaagcgcgc ctgcagcata ttatgtttgc ccgtaacagc
     5341 cagaaaatgc acatcccgga gaattttacc gtctcgtggg attactcgtt atgcaaacgc
     5401 gccattgatg aaaactgctt tttcagcgat gaagtccctg accgttgggg cgactgtatt
     5461 gcggcacgca atcttggcat caccacattt ctgagcacgc caattcactt accggatgga
     5521 tcattctatg gcacgctttg cgccgccagc agtgagaagc gccagtggag tgaacgcgcg
     5581 gaacaggttt tacagttatt cgccggactg attgcacaat atattcaaaa agaggcactg
     5641 gttgaacagc tgcacgaagc caatgctgcg ctgattgcgc aatcgtatac cgactcgtta
     5701 accgggctac cgaatcggcg ggcgattttt gaaaatctga cgacgctgtt ttccctcgcc
     5761 cggcatctta accataagat aatgatcgcg tttatcgatc tggataactt caaattaatc
     5821 aatgatcgtt ttggtcataa tagtggcgat ctgtttctca ttcaggttgg cgagcgcctt
     5881 aatacgctcc agcaaaatgg cgaagttatt ggtcgtctcg gtggtgatga gtttttggtt
     5941 gtttcactaa acaacgagaa tgcggatatt tcgtcgctgc gagaacgtat tcaacagcaa
     6001 atacgtggag aatatcactt aggtgatgtt gatttgtatt atcccggtgc cagtcttggc
     6061 atagtagaag tcgatcctga aacaaccgat gcagacagtg ccctgcatgc tgccgatatt
     6121 gcgatgtatc aggagaaaaa acacaaacag aaaacacctt ttgtcgcgca tccagcgcta
     6181 cattcctgag gcgtattcac atccttttga ttggtgataa catgcgaatc ggtattattt
     6241 ttccggttgt aatcttcatt acagcggtcg tatttttagc atggtttttt attggcggct
     6301 atgctgcccc gggagcataa agatgaaaaa aacaacgatt attatgatgg gtgtggcgat
     6361 tattgtcgta ctcggcactg agctgggatg gtggtaacgt cacctctaaa aaatagcaaa
     6421 ggctgcctgt gtgcagcctt tgtgcaattt aagcgttaac ttttaatctt cctgtagata
     6481 aatagcacga caatcgcacc aataacggct gaagcatttc gtctgcagtc tgaaccgcag
     6541 gcaaccattt ttcagcaagt ctgcgctggg aatcgctggc tttatcccat ttgctccaca
     6601 gtgcctcacg atcgtaatcg tgcgcttcca gcgtggggcg ggcgatttca aaatacccct
     6661 ggcttgtttc tatttgctca gattttaata acaactcaac acgtgctgcc gctggtagaa
     6721 ctgatatatg aaattccaaa acctttgaac ccgtacgctt gcgacgtagt tctggatgat
     6781 tggctgcaag cttttcaaga ttgcggcgaa tact
//
LOCUS       DAJTYG010000152         6802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTYG010000152 DAJTYG010000000
VERSION     DAJTYG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.152"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            342..584
                     /locus_tag="OO568_003679"
     CDS             342..584
                     /locus_tag="OO568_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642844.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAVTGGGDGSNTGTTTTTTTSTQ"
     gene            698..1336
                     /locus_tag="OO568_003680"
     CDS             698..1336
                     /locus_tag="OO568_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6642845.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQSTLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTIIKAAKQ"
     gene            1333..2086
                     /locus_tag="OO568_003681"
                     /pseudo
     CDS             1333..2086
                     /locus_tag="OO568_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
     gene            2086..4182
                     /locus_tag="OO568_003682"
     CDS             2086..4182
                     /locus_tag="OO568_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6642846.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ERMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4229..4507)
                     /locus_tag="OO568_003683"
     CDS             complement(4229..4507)
                     /locus_tag="OO568_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642847.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4508..4651
                     /locus_tag="OO568_003684"
     CDS             4508..4651
                     /locus_tag="OO568_003684"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642848.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4777..5187
                     /gene="psiE"
                     /locus_tag="OO568_003685"
     CDS             4777..5187
                     /gene="psiE"
                     /locus_tag="OO568_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6642849.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5231..6705)
                     /gene="xylE"
                     /locus_tag="OO568_003686"
                     /pseudo
     CDS             complement(5231..6705)
                     /gene="xylE"
                     /locus_tag="OO568_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
BASE COUNT         1707 a         1704 c         1788 g         1603 t
ORIGIN      
        1 aaaacaatgc cgacaataag tcggcattgt tttatcagat aaatcccctt gtctgtgatt
       61 taacggaaat cataccgtga ggttaatcct aaaatagatt tttaatcgtt gtttatttcg
      121 gagaatacgc agattaatta cttttgtttt tattttaagt ttatgatttt tattgttatt
      181 taaatataag ttgaaactta tatttgatat tcattccaat tatcctaaaa ctccatcgct
      241 aattcctcgc gccgtaattc gcatgcttta gttgtgtata ctcgatcccg cccgaaatgt
      301 ttttgggtaa atctccattc attcaatgaa gggaaattgt tatgaaaaaa gttctgtatg
      361 gcatttttgc catatctgcg cttgcggcga cttctgcgtg ggctgcacct gtacaggtgg
      421 gcgaagcggc agagtcggca gcaacgtcgg tttcggcggg gagttcctcc gcgaccagcg
      481 tcagcaccgt aagctcggcg gtgggtgtcg cgctcgcggt aaccggtggc ggtgatggtt
      541 ctaataccgg gaccaccaca accacgacca ccagtaccca gtaataaagt atgtatcccc
      601 aaaataattt gagtcattgc atctgtggct tgaagtatga agggatttac cataaccaca
      661 ctccggtgtg gttattctgc ccctctggag aagagtcgtg aagcgacctg cactcattct
      721 tatctgcctg ctattacagg cctgttcagc cacgactaaa gagctgggca attcactgtg
      781 ggacagtctg ttcggcacgc caggcgtaca gctgacggac gatgatattc aaaatatgcc
      841 ctacgccagc cagtacatgc agcttaatgg cgggccgcaa ttgtttgtgg tgctggcctt
      901 cgctgaagac ggacaacaaa aatgggtcac tcaggatcaa tccactcttg tcacacaaca
      961 tggtcgtctg gtgaagactt tgcttggcgg cgacaacctg attgaagtga ataaccttgc
     1021 cgccgacccg ctgattaaac ccgcacaaat tgttgatggt gcaacatgga cgcgcacgat
     1081 gggctggacc gagtaccagc aggtacgcta cgccaccgca cgctcagtct tcaaatggga
     1141 tggcaccgat accgtcaagg tcggcagcga tgaaaccctg gttcgcgtgc tggacgaaga
     1201 agtctccacc gaccaggcgc gctggcataa ccgctactgg atcgacagcg aagggcaaat
     1261 tcgccagtcg gaacagtatc tcggcgcgga ttatttcccg gtgaaaacca ctatcatcaa
     1321 ggcggcaaaa caatgattaa acaaactatt gtcgcgttgc ttttgagtgt gggagcgtca
     1381 tcggtcttcg cggcaggaac cgtcaaggtg ttcagcaatg gcagcagtga ggccaaaacg
     1441 ctaacgggcg cagagcattt aatcgatctg gtagggcaac ctcgactggc aaacagctgg
     1501 tggcctggtg cggtgattag cgaagagctg gcaacggcgg cagcattgcg tcagcagcag
     1561 gcgttgctga cacggctggc agagcagggc gcagattcca gcaccgacga tgccgccgcc
     1621 attaacgcct tacgccagca aatccaggca ttgaaagtga caggcaggca aaaaatcaat
     1681 cttgatcccg atatcgtccg cgttgccgaa cgcggtaacc cgccgttgca gggcaactac
     1741 acgctgtggg tcggaccacc gccgtccacg gtcacgttgt tcgggcttat cagccatcct
     1801 ggcaatcagc catcctggca atcagccatt cactccaggt cgcgacgtgg cgagctatct
     1861 ctctgaccag agcctgctca gcggtgcgga tcgcagctac gcgtgggtcg tttacccgga
     1921 cggacgcacg caaaaagcgc cggtggctta ctggaacaag cgtcacgtag agccgatgcc
     1981 cggcagcatt atttatgttg gcctcgcaga ttccgtctgg agtgagacgc ctgatgccct
     2041 taacgccgac attcttcaga ctctgacgca gcggataccc caataatgaa aaaaagacat
     2101 ctgcttagct tactggcgct gggcattagc acagcttgct acggcgaaac atatcctgcg
     2161 cccattggtc cgtcgcagtc ggatttcggt ggcgtaggat tattacaaac tcccaccgag
     2221 cgtatggcac gggaagggga gttgagtctg aactatcgcg ataacgatca gtaccgttat
     2281 tactcagctt cagtgcaact cttcccgtgg ctggaaacaa cgctgcgcta caccgacgtg
     2341 cgcacccggc agtacagcag cgtcgaagcg ttctctggcg atcaaacgta taaagataaa
     2401 gccttcgatc tcaaactgcg tttgtgggaa gagagttact ggctgccgca agtggcggtt
     2461 ggtgcgcggg atatcggcgg tacggggctg tttgatgcgg aatatcttgt tgccagcaaa
     2521 gcctgggggc cgttcgattt tacgctcggc ctgggctggg gatatctggg caccagcggt
     2581 aatgtaaaaa atccgctctg ttcagccagt gataaatatt gctatcgcga taacagctac
     2641 aaacaggcgg gatctatcga cggtagccag atgttccacg gtcctgcctc actgtttggc
     2701 ggcgtggaat accagacgcc ctggcaaccg ctgcgtctga aactggagta tgaaggtaat
     2761 aattatcagc aggattttgc cgggaagctg gagcaaaaaa gtaagtttaa cgtcggggcg
     2821 atttatcgcg ttaccgattg ggccgacgtt aaccttagct atgaacgtgg caacaccttt
     2881 atgtttggcg tcacgctgcg caccaacttt aacgatctgc gcccgtccta catcgataac
     2941 gcccgcccgc aatatcaacc gcagccgcag gatgccattt tgcagcattc ggtggtggcg
     3001 aatcagttaa cgttgctgaa atacaacgcc ggacttgccg atccgcagat ccaggcgaaa
     3061 ggcgatacgc tgtatgttac cggcgagcag gtgaaatatc gtgattcgcg cgaagggatc
     3121 atccgtgcta atcggatcgt gatgaacgat ctgccggatg ggatcaaaac gatccgcatt
     3181 acggaaaatc gccttaacat gccgcaagtg acgacggaaa ccgatgtcgc cagcctgaaa
     3241 aatcatctcg ccggagagcc gttgggccac gaaacgacgc tggcgcacaa acgcgtcgag
     3301 ccagtggttc cgaagtccac cgagcagggc tggtatatcg acaaatcacg ctttgatttc
     3361 catatcgatc cggtgctgaa ccagtcggtc ggtggcccgg aaaactttta catgtatcag
     3421 ctgggcgtga tgggaacggc agatttgtgg ctgacggacc atctactgac cactggcagc
     3481 ctgtttgcaa atcttgccaa caactacgac aagtttaact acaccaatcc accgcaggac
     3541 tcgcacttac cgcgcgttcg tacccatgtg cgcgagtatg tgcagaacga tgtctatgtg
     3601 aataacctgc aagccaacta cttccagcat ctgggcaacg gcttctacgg tcaggtctat
     3661 ggaggttatc tcgaaaccat gtttggcggt gcgggggccg aagtgttgta tcgtccgctg
     3721 gacagtaact gggcgtttgg tctggatgcc aactacgtta aacagcgcga ctggcgtagt
     3781 gcaaaagata tgatgaaatt caccgactac agcgtgaaaa ccggacatct gaccgcctac
     3841 tggacgccat ctttcgccca ggacgtgttg gtgaaagcca gcgtcgggca gtatctggca
     3901 ggggataaag gcggcacgct ggagatcgct aaacgctttg acagcggcgt ggtggtgggt
     3961 ggctatgcca cgatcaccaa cgtttcgaaa gaggagtacg gcgaagggga cttcaccaaa
     4021 ggcgtgtatg tctcggtacc gttggatctc ttctcgtctg gcccgacacg cagccgtgcg
     4081 gcgattggct ggacgccgct gacgcgtgac ggtggtcagc aacttgggcg taagttccag
     4141 ttgtatgaca tgaccagcga ccgtagcgta aatttccgct aagtcatggg aaaggtgcca
     4201 gttttcgcac tcactggcac cctcattctt aatgacctgt tgagccatca ggaacgcagt
     4261 tcacccccgg tttacgatct ggtgtgtaaa ggcgaaaaca ttctgtcttc ctgtccgtcg
     4321 atatcggttg caggtctggt tgcctgagcg actgggccat cattgggctg gagacattgc
     4381 tttgcggtag gtcggcttta tcagcagtac cgagcggacc agcataagca ggaagaacag
     4441 agactgataa catcaaagca gcaaaataag gcttcatttt taccaccttt atcaggttac
     4501 gtttcatttg ttccagagga acattgtcga tttttcgcgc attgctggtg gctgggaatc
     4561 acctgaatgg gtgatttttg aattaccggc tttggtgcgg tttgtcttgc cggatgcgcc
     4621 gccaggcgcg gcttatccgg cctacgggta ggtatatccg gctttggtgg aggcgcgctc
     4681 caaatccagg ttgaacaaaa catacacaaa aaatatagat ctccgtcaca tttttgcgtt
     4741 atacaggaag ctcgccactg tgaaggaggt actgctatga cgtcactctc tcgtccgcgc
     4801 gtggagttta tctccaccat tttgcagacc gtactcaatc ttggcctgct gtgcctcggc
     4861 ctgattttgg ttgtcttcct cggcaaagaa acggtgcatc tggctgatgt gctgttcgcg
     4921 ccagaacaaa ccagcaaata tgagctggta gaaggactgg tggtttactt tctctatttc
     4981 gaatttatcg cgctgattgt gaagtacttt cagtccggtt ttcacttccc gctacgttac
     5041 tttgtctata ttgggatcac cgccattgtg cgcttgatca tcgtcgatca taaatcgcca
     5101 ctggatgtgt tgatctactc ggctgcgatc ctgttactgg tgatcaccct gtggctgtgt
     5161 aattcgaaac ggctgaagcg ggagtaaaaa gacagcacgc cgaaatggcg cggcgtgctg
     5221 gacaggaaga ttacagcgta gcagtttgtt gtgttttctt cgtttccggt tcccagagcg
     5281 cttccagctc ctcaagggtt ttacctttgg tttccgggac aaatttccac ataaacagtg
     5341 ctgccagaac gcccatacaa ccgtaaatcc agtaggagaa accgttgtgg aaatgggcca
     5401 ccagccagga gtttttgtcc atcatcggga aggtccagga gacgaagtag ttcgccagcc
     5461 actgggccgc caccgcgatt gccagcgctt taccacgaat agcattcggg aagatttccg
     5521 acagcagtac ccagcatacc ggaccccagg acatggcaaa ggcggcaaca tagaacagca
     5581 tcgacagtag cgccacaata cccgagcctg agtgtaaaac gcggtactga ggctaaacat
     5641 accgattgcc attccgagtg cgccgataat ttgcagtggc ttacgaccaa atttatccac
     5701 agtcataatt gccagtacgg taaaggtgag gttgataact ccgacaataa tggtctgcaa
     5761 cagcgcgata tccgtgctgg cccccagcgt tttgaacact tccggcgcgt agtacagcac
     5821 cacattgatg ccgacaaatt gctggaagat ggagagcata acgccgatta caatcacgcc
     5881 cacgccaaac atcagcagac gaccaccggt tttgcggcca tgatccaggg agtgtttaat
     5941 ttcctgtact gcctgagttg caagcgtgtt gcccataatt ttgcgcagga tactttccgt
     6001 ctgttcttgc ttgccgcgtg acatcagcca gcgaggactt tctggcacgg tatacagcag
     6061 cattaagaac agcagtgcag ggatacattc cgaggcaaac atataacgcc tgccgtcagt
     6121 attcagccag ctggcatcac cggaacgggc aataaaatag tttacgcagt aaactaaaag
     6181 ttgcccgaaa ataatcgcaa actggttaaa tgaaaccagt tttccgcgaa tatgagctgg
     6241 agccagttcc gcaatataca ttggtgaaag cattgaggct aaaccaacgc caataccgcc
     6301 aataatacga taaataacaa attccgggac ataacctgcc agataaatag gtactgtgtt
     6361 gtccgggttt atagaggtaa aaccaagttc tggccaggca gaacctacac cagaaataaa
     6421 aaacaggaca gcagaaatct taagtgaatc acgacggccg aagcggttac tgcaataacc
     6481 accgagggca ccgccgatga tgcaaccaat cagagcgctg gctacgcaaa atcctaacag
     6541 ggagttggca gcggattcac ttaagttttg tggagcaaca aagacggtat tgagtgactc
     6601 aacagtaccg gaaataacgg cggtgtcgta gccaaataat aaaccaccta atgtagcgac
     6661 taaggtaatc gaaaatatat aactggaatt atactgggta ttcattcaga cctgccttag
     6721 accattctga tcttttatca gtgcattgat gttggaattg tcaggcagtt aaggcaatta
     6781 tcatttttta caatgcgttt ac
//
LOCUS       DAJTYG010000153         6769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTYG010000153 DAJTYG010000000
VERSION     DAJTYG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.153"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..3512
                     /locus_tag="OO568_003687"
                     /pseudo
     CDS             1..3512
                     /locus_tag="OO568_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3522..6769
                     /locus_tag="OO568_003688"
                     /pseudo
     CDS             3522..6769
                     /locus_tag="OO568_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1794 a         1779 c         1629 g         1567 t
ORIGIN      
        1 gaaagataat tctttgcaac cttcccaaac ggtaacggac tctctcaagg tagcggactt
       61 atccaccatt attcaggaac aaccctcttt cgtagcagaa acccgtgcgg ctgacaaaaa
      121 tacagatgct gttttacctt ggctggcgaa agatattgct gtcgtttttc cgcccgaagt
      181 tgtacacacc acgttaagtc atcgccgctt tcccggagtt cctgttcagc aagcagacaa
      241 attgacccaa ttacgtcgct tagcctgtag cgtgtcgcaa cgggataata aaacagccac
      301 atttgacttc agcacctgtt ctttagaatg gcaaaacacc gtcgcccaag cgatcagtca
      361 gatcgacggc ctgaaaacaa cacagctacc atcaccagta atggctgtac tcacggcact
      421 tgaaatgaaa tgcacaagat ataaagtgcg tgaggatgtt atggatcaga tcgtccagga
      481 aggcggtctg gaatatgcta ctgatgtaat aatacacctt caacagattg atattaaatg
      541 ggattatgcg aataatgtca ttattattct gccgtctggc attgcacctg actacttgga
      601 gcaatattcc agatttgaat tacgcctacg taaacattta tcactggcgg aagagtctct
      661 ctggcaaaaa tgtgcacaaa aacttattgc cgcaattcca catattccag aatggcggca
      721 accattaatt gctttgttat tacccgaaaa accagaaatt gcacatgaaa ttgcccagcg
      781 tctactgggg caaaaaaaat taccctcgct tgagtggtta aaaatagtgg cgactgatga
      841 gcacattctt gcctcattag aaaaatatca cgagccatat gccatttttg atgattacta
      901 ttgtggtgcg atatggtcag ccaccgtatt acaggagcaa ggtgttgcag ccctgccccg
      961 atttgctccc tatgccgcaa gtgactactg cgccgatgtg ttgcgtcata tcaatcatcc
     1021 gttcgcatta acactgctta tacgtgtagc cgggcatact aaacgctgtc acgatcgaat
     1081 gacgaaagcc tgcgctgcgt tcccacacgc agcactggca gaacaggtca ttccctggct
     1141 ttcgacaccc gcagttgcag tgctgaaatc atgccagcac caactgacac agccctcaaa
     1201 ccatgccagc gccgatctac tgccagccat agtagtctcc ccaccatgga tttcgaaaaa
     1261 gaaaaaatcg ccgattccgg tgctggattt agcgccatta ggcatcgagc caatctgtta
     1321 tctgacagaa gaaatcagta atcaactttt ggcgaaatat atctggtatt caaaacacat
     1381 cacggttagc catgaagaaa gtactaccaa cctgttggca aggatgggtt ttcaacgacg
     1441 gatcgctggt acatatatta aagctcccga agcggtagtt gaggcatggc taaatgaaga
     1501 ttattcaacc ttactaagtg aatttaaggt gtttcattca cctaccgggc attattggca
     1561 gttggggatt ttgacaacat tgccgctgga ggctggagaa agcagtaaaa gcatggaatg
     1621 cccttactct atctccacat accgataccg aatacgccat gttacatttt ggactcaaag
     1681 gtttacctgg tttagttaac tcaattacac gctatccgca ggaagccttg cccatcacga
     1741 attacttcgc agcgagtgag ctggctcctg ccgtcgcccg tgccttcaac aaactcaaaa
     1801 cgctacgcga aaacgcccgc acctggctgt tgaaataccc ggaacatgcc cttaccggcc
     1861 tgctgcctgc ggcgctcggc aaagccggtg aagcacaaga taacgcccgc gctgccttgc
     1921 gtatgcttac cgaaaacggt catcagccat tactgcaaga aatcgcccgg cgttataacc
     1981 agtcggaagt aacagacgcg gtgaacgctc tgcttgcgct cgatccctta gataatcacc
     2041 cgacaaaaat ccccactctt ccgacctttt atcagccatc gatctggacg cgcccggtat
     2101 taaaagcaaa cgcccaatca ctgccagata acgccctcct ccacctcggc gaaatgctcc
     2161 gcttccctca ggaagaggct ctgtatccgg gattattgca ggtgaaagac gcctgtaccg
     2221 ctgactctct ggctgaattt gcctgggatc tgtttaccgc ctggcagacc gctggcgcgc
     2281 cgtcgaaaga gagttgggcg ttcactgcgt taggcgttct cggtaacgat gacaccgccc
     2341 gcaaactgac gccattaata cgcgcctggc ctggtgaatc ccagcataaa cgcgccaccg
     2401 ttgggttgga tattctcgcc gctatcggta gtgatatcgc cctgatgcag cttaacggca
     2461 ttgctcagaa actgaaattc aaagcattac aggagcgggc aaaagaaaag attgccgaca
     2521 ttgccgagag ccgcgaactc acggtagcgg agcttgaaga tcggttagca ccggatctcg
     2581 gtctggatga taacggttcg ttgctgctgg attttggccc acgccagttc accgtcagct
     2641 ttgatgaaac cttaaaaccg tttgtgcgtg atgtttccgg cagccgcctg aaagacctgc
     2701 caaaaccaaa caaaagcgat gatgagtcac aggcgaacga tgctgttaac cgctacaaat
     2761 tgctgaaaaa agatgcgcgt accgtcgccg cccagcaggt agcaaggctt gaatccgcca
     2821 tgtgcctgcg tcgccgctgg tcgccggaaa acttccaact cttcctggtt gtgcatccgc
     2881 tggttcgcca cttaacccgc cgtctgattt ggggcgttta tagcgccgac aaccagctac
     2941 tggcctgttt ccgggtagcg gaagataaca gctacagcac cgctgacgat gatcttttca
     3001 ccctgccgga aggcgatatc tctatcggca ttcctcacgt tctggaaata tcaccgacgg
     3061 atgctgtcgc ctttggtcag ctttttgccg actacgaact gctaccaccg ttccgtcagc
     3121 ttgaccgtaa cagttacgcc ctgacagaag ccgagcgcaa cgccagcgaa ctgacccgct
     3181 gggcaggcag aaaatgcccg agcggccggg tcatggggct ggcaaataaa ggctggataa
     3241 agggcgaacc acaggatgga ggctggatcg gctggatgat caaacctctc ggtcgctggt
     3301 cgttaatcat ggaaatcgac gaaggttttg cggtgggtat gtcaccagcc gaacttagcg
     3361 ctgagcagct cttaagcaag ctgtggctat gggaaggcaa agcggaaagc tatggctggg
     3421 ggagtaattc aacacaggaa gcgcagttct ccgtactcga tgccatcacc gccagcgagc
     3481 taattaacga tattgaagcc ctgtttgaat aaggaaagag catggacaag gaattaccgt
     3541 ggctggcgga taacgcccaa ctggaactga aatataaaaa aggcaaaaca ccactcagtc
     3601 atcgcaattg gccgggcgaa ccagtgcctg ttatcactga aagcatcatt cagacactgg
     3661 gtgatgaatt gctacaaaaa gctgagaaga aaaaaaacat tgtctggcgt tatgaaaatt
     3721 tttcaccgga gtggcagtcc gccattacgc aggccatcaa cttgatcggc gaacacaaac
     3781 cctcagtccc ggcccgcaca atggcggcac tagcctttat cgcgcaaaat gacaaccaac
     3841 agttgctcga cgaaatcgtc caacaagagg ggctggaata tgcgactgag gtggtgattg
     3901 cacgccagtt tattgcacgg tgttatgaga gtgatcctct ggtagtcaca ttgcagtatc
     3961 agaacgagga ctatggctat ggttatcgct cagaaaccta taacgaattc gatctccgac
     4021 tgcgtaaaca cctctctctg gcagaggaaa gctgctggca gcgttgcgcc gacaaactca
     4081 ttgccgcact gccaggaata accaaagttc gccgcccttt tattgcgctg atcctccccg
     4141 aaaaaccaga aatcgccaat gagttggtaa gccttgaatg cccgcggact cattttcatt
     4201 ctaaggagtg gttaaaagtt gttgctactg accccaaagc ggtgagaaaa ctcgaacgct
     4261 actggagcca ggatatattt agcgatcgag aagccagcta catgtcgcat gaaaaccact
     4321 tcggctacgc ggcctgcgct gcccttttgc gcgaacaagg actggcagtc gttccgcgcc
     4381 tcgcgatata tgcccataaa gaagattgcg gcagtctgct ggtacaaatt aaccatccgc
     4441 aagtcatccg caccttgctg ttagttgctg ataaaaacaa acccagcctg caacgtgtgg
     4501 ctaaatacag taaaaacttc ccccatgcga cactcgccgc actggcagaa ctgctggcgt
     4561 taaaagaacc accagcccgc ccaagttatc caatcatcga agacaaaaag ctgcctgcac
     4621 agcaaaaagc tcgagatgaa tactggcgta cgctgttaca aacgctgatg gcatcgcagc
     4681 cacaactggc agcagaagtg atgccgtggt taagtactca agcccgggca gtgctgaata
     4741 gttatttatc ggcaccgccc aaaccagtta ttgatagcac cgataacagc caaatgcctg
     4801 aaatcctcgt ttcaccacca tggcgtggga gaaagaaaaa gacttccata cgtcactttt
     4861 cgctgcctga tcttacgctt gcaccgcaaa cgtacaggtc gccgaataaa cactggacgc
     4921 cggaacagca aactgcaatt gaccatttta gcgccatgtc ttttgacgaa cgtcttgcta
     4981 cgagaggagc ggatatcttc ttgcgggaat taggttttga cgttcatagc ctggaattta
     5041 ataagcgtct tcttaatgga cagttggatg cctttgatgc tcagcaactg caagaataca
     5101 catcaatagg actattgcgt acttatcacg cgcatcaggc caaatctaaa cagtaccggg
     5161 aagacgccac caatgcatta ctggcacaag atagcattgc gttgatggga gggtggcgaa
     5221 cttttcagca cggattttac tatggtaatg aacagcgttg ggggatttgg aatctgtacc
     5281 ttatcgcgca gatgccgcgc gaaatggcgg tatcgtgctg gcaacaaatt gtcgaggctg
     5341 attttcatta caccggggtg gaatatttgt taagtgtgct gggtaccgat gctttgcctg
     5401 gattaaacac cgcctttgcc cgacatccaa aagagatatt tccgctgctg attcatttcg
     5461 gcgcaacaga actggccctg cccattgccc gcgtctggca cctttttgcg ggccagcgta
     5521 atctggctcg ccagtggatt ttacaatggc cggaacatac ggctactgca cttatccctc
     5581 ttgtcttcac caaatctagc gataaaagcg aagctgcatt acttgccctg cgtttacttt
     5641 acgaacatgg gcatggcgaa ttgctacaaa ctgtagccaa tcgttggcag cgtacagatc
     5701 tatggcctgc cctggaacaa ttacttaaac agagtccaat agagatttac ccggcacgca
     5761 ttccaaaagc ccctgatttc tggcatccgc aaatgtggtc caggccgcgc cttatcacta
     5821 ataatcaacc tgttaccgat gacgctctgg aaattatcgg cgaaatgctg cgctttaccc
     5881 aggggggacg tttttatagc gggctggaac aactgaaaac gttctgccag ccacaaacgc
     5941 tggcagcttt tgcctgggat ctcttcactg cgtggcaaca agctggtgcc cccgcaaaag
     6001 acaactgggc atttctggcg ttaagtctct ttggtgacga aagcacggca cgggatctaa
     6061 cgacacatat cctcgcctgg ccacaagaag gcaaatctgc ccgtgcggtc agtggcctga
     6121 acatccttac cctgatgaat aatgatatgg cgctgatgca gctgcatcat atatcgcaac
     6181 gggcgaaatc ccgcccctta cgtgataacg cggcggaatt tcttcaggta gtcgcagaaa
     6241 atcgcgggct aagccaggaa gagttagcgg acagattagt cccaacactg ggccttaatg
     6301 atccgcaggc gttgagtttt gattttggtc cccggcagtt taccgttcgc ttcgatgaaa
     6361 acctcaaccc ggttatcttt gatcagcaaa acgttcgcca gaaaagtgtt ccccggttgc
     6421 gcgccgatga cgatcaactg aaagcgcccg aggcactggc ccgactaaaa gggttaaaaa
     6481 aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct tgaagctgcc ctacgtacca
     6541 cccgacgctg gtcgctggca gattttcatt ctctgtttgt taatcatccc tttacccgtc
     6601 tggttaccca gcgattaata tggggggtta tccggcaaat gaaccgcgtc gtttactcaa
     6661 cgcctttcgt gtggccgcag agggggagtt ctgcaatgcg caagatgagc caattgacct
     6721 gcctgcggac gctctgattg gcattgccca cccgttagaa atgacagca
//
LOCUS       DAJTYG010000154         6768 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTYG010000154 DAJTYG010000000
VERSION     DAJTYG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.154"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO568_003689"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO568_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6642850.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO568_003690"
     CDS             2089..2238
                     /locus_tag="OO568_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642851.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="OO568_003691"
     CDS             complement(2983..3423)
                     /locus_tag="OO568_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6642852.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="OO568_003692"
     CDS             3641..4030
                     /locus_tag="OO568_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642853.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4478
                     /locus_tag="OO568_003693"
                     /pseudo
     CDS             4074..4478
                     /locus_tag="OO568_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4585..5421)
                     /locus_tag="OO568_003694"
     CDS             complement(4585..5421)
                     /locus_tag="OO568_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6642854.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5421..5696)
                     /locus_tag="OO568_003695"
                     /pseudo
     CDS             complement(5421..5696)
                     /locus_tag="OO568_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5771..6244)
                     /locus_tag="OO568_003696"
     CDS             complement(5771..6244)
                     /locus_tag="OO568_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR6642855.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(6265..6768)
                     /locus_tag="OO568_003697"
                     /pseudo
     CDS             complement(6265..6768)
                     /locus_tag="OO568_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1578 a         1752 c         1771 g         1667 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcttg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct tgatcgcggc
     4621 cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg cggaaaggca
     4681 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
     4741 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
     4801 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga cgggatctat
     4861 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
     4921 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
     4981 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
     5041 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
     5101 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     5161 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     5221 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     5281 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     5341 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     5401 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     5461 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     5521 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     5581 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     5641 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcgtcg
     5701 ctatcaatcg cggaaaaagg cgtagtgcgc gaagcgaacg aagctttttg ccgtcgattg
     5761 catagctttg ttaacccttt ttccaaattt gatagcaata gttaatgttt gaagtaaagt
     5821 gttgctcaaa aacaacttcg aaggtatttg gaatactcgg gaagaaaaca tccccctctg
     5881 gctcgatgtc gatcgtcgat aagtggagcg tagaggccat gggtaatgtt tctcggtaaa
     5941 tttctccgcc accagacact ataacgtgac cggtgaattc agctagcctg tccatggcct
     6001 cttcgattga ctgaaataca actacattgt catcatttga tgtccaacct gagcgggtaa
     6061 cgaccgcgta tttcctattg gggagtgcgc ccatagattc aaacgtcttg cgacccacca
     6121 gaagccactg attgtaggtc aatgctttaa aaagtagctg ctcccctttc gcggaccagg
     6181 gtatgtctgg accgcaacca atcacgccgt ttttcgcttt cgcagctatc aatgatactt
     6241 tcaaggttct catcctgggt taacggtgcg ctcttggtcg agccgcaccg gtagctctcg
     6301 ttcgacacga gccaaaagat cgagctgcgg cgtactcttg tcctcgtccg gtaagaagtc
     6361 gggattgacg gcattgcggg acaccacatc aacggcgcgt ccgaacattc gcgacagcct
     6421 gcgctcaaac ggacattgat caaccgatag gctgtgaaca gcgccaagtt gctgccccat
     6481 tgacggccac gctttgagca aatccgctcc agacagatca gccgccggta ctcccggaat
     6541 tgccgttatc accaagcatg caccctcctg ttcctcctgc cagttgatga cctcggggca
     6601 agccacacct cgacctttga gccaaatgag gcggtcacgc tctccagcga gctcaccgcg
     6661 gcgggaagca ggtgcgattt tcgcgaaggc atgcccgtca ccacgtcgaa aaacaaaatc
     6721 accagattct ccgcctctga caggcaacca gtcagaatgc gattcacc
//
LOCUS       DAJTYG010000155         6765 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTYG010000155 DAJTYG010000000
VERSION     DAJTYG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.155"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            239..2671
                     /locus_tag="OO568_003698"
     CDS             239..2671
                     /locus_tag="OO568_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR6642856.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGLFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEALI
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2738..4207
                     /locus_tag="OO568_003699"
     CDS             2738..4207
                     /locus_tag="OO568_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6642857.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4207..5868
                     /locus_tag="OO568_003700"
     CDS             4207..5868
                     /locus_tag="OO568_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR6642858.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6089..6430
                     /gene="symE"
                     /locus_tag="OO568_003701"
     CDS             6089..6430
                     /gene="symE"
                     /locus_tag="OO568_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR6642859.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6477..>6765)
                     /locus_tag="OO568_003702"
     CDS             complement(6477..>6765)
                     /locus_tag="OO568_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642860.1"
                     /translation="RLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFHEL
                     VDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
BASE COUNT         1993 a         1606 c         1546 g         1620 t
ORIGIN      
        1 ttcttgttcg aaagaaacgc aactataaga tttttatata ttttgttaga gttgatatgg
       61 ttcctgtaac ttctgtttat ctgtctctga tatgcccaga ttaattcaag caagatataa
      121 cagagatatc aatgtgatct ctgctacgta ctttacgttg aaacaccgcc ctaaatcata
      181 gccaggctat tgattttatg tacaatcaga atcgtcctga taaaagcgag aacaccccat
      241 ggcggaactg aacctaagta acctgacgga agcagatatc attaccaaat gcgttatgcc
      301 agccattctc aatgcaggct gggacaacac aacgcaaatc agacaggagg tcaaactccg
      361 ggacggtaaa gtcatcgtac gtggtaaagt tgcggcacgc agaacggtaa aatctgctga
      421 catcgtgctg taccacaagc caggcattcc cttagccgtg attgaagcaa aagccaacaa
      481 acatgaaatt ggcaaaggga tgcaacaggg cattgaatat gcgcgcctgc tggacgttcc
      541 ttttgttttt gccaccaacg gtgatggctt tatcttccgc gatgccaccg cagccgaagg
      601 tgaatgcctt gaaaagcaaa taacgctgga tgacttcccc tcccccgctg aactctggca
      661 aaaattctgc ctctggaaag gatacacaca agctcagctt ccggtgatta ctcaggacta
      721 ttacgacgat ggcagcggca aatcgcctcg ttattatcaa cttcaggcaa tcaacaaaac
      781 cattgaagcc gtctccaacg ggcaaaaccg cgttctgctg gtcatggcga ccggaacagg
      841 gaaaacctat accgcattcc agatcatctg gcgcctgtgg aaatcaaaaa ataaaaaacg
      901 cattttgttc cttgccgatc gcaacattct ggtcgaccaa accaaaaata atgatttcca
      961 gccatttggt acggcaatga ccaaagtcag cggacgcacc attgatcccg cttatgaaat
     1021 tcacctcgcg ctctatcagg ctataactgg cccggaggaa gaccaaaaag cgtttaaaca
     1081 agtcgcacca gatttcttcg atctgatcgt gatcgacgaa tgccatcgcg gcagcgcatc
     1141 cgaagacagc gcctggcgag agatccttga ttatttcagt tccgccaccc aaattggctt
     1201 aaccgccacg ccaaaagaga cgcatgaagt ctccagcacg gattacttcg gcgatccggt
     1261 ttacgtctac tcactaaaag aagggatcga agacggcttc ctcgctcctt ataaagttgt
     1321 ccgtgttgat attgatgtcg acctgcaagg ctggcgccca accaaagggc aaactgactt
     1381 aaacggcgaa gtgattgacg atcgtatcta taaccagaaa gatttcgatc gcacgatggt
     1441 gatcgacgaa cgcacagaac tggttgccag aaccattacc gactacctca agcgcaccaa
     1501 tccgatggat aaaaccatcg tcttctgtaa cgacatcgat catgcagaac gtatgcgccg
     1561 cgccctggtt aatctcaacc cgcagcaggt gaaaaagaac gacaaatacg tcatgaaaat
     1621 caccggcgat gatgaaattg gcaaagctca gttggataac ttcatcaacc cgaaaaaacc
     1681 gtacccggtt atcgcgacca cttcagagct gatgaccact ggtgtggatg ctaaaacctg
     1741 caaactggta gtactagacc agaacatcca gtcgatgacc aaattcaagc aaattatcgg
     1801 tcgtggtaca cgcatcgacg aacgttacgg caaactctgg tttaccatcc tcgactttaa
     1861 aaaagccacc gaactgtttg ccgatgagcg tttcgatggc attcccgaaa aagtcatgga
     1921 taccacacca gaggatatcg ccgatccaga atctgatttt gaagagaaac tcgaagaaat
     1981 cagcgaacat gacgacgaac aggtaacagg cgttgatgaa ccgcctgcgc caccatacca
     2041 ggttaccgat accgatgatg tcggcctatt tccggaagaa gacgagaaga aaatccgcaa
     2101 gtttcacgtc aacggtgtag cggtgggcgt tattgcacag cgcgttcagt attacgacgc
     2161 cgacggtaaa ctggttaccg aatcctttaa agattacacc cgcaaaacac tgctcaaaga
     2221 atatgcctcg ctggatgact ttacccgcaa gtggcaggac gccgatcgca aagaagcgct
     2281 cattcacgag ctggagcaac aggggatcat ctgggaagta ctggcagaag aagtcggtaa
     2341 agatctcgac ccgttcgaca tgctttgcca cgtagtgtat ggtcagccgc cgttaacccg
     2401 caaagagcgc gccgagaacg tgcacaagcg gaactacttc acaaaatact ctgaagcagc
     2461 gcaagccgtg ctcgataatc tgctggataa atacgccgat gcgggcgtac aggagatcga
     2521 aagtattcag gtgctgaaac ttaaaccatt cgacagcatg ggcaccttac cggagattat
     2581 taaaaccgga tttggcgacc gtaacgggta taatcaggcg ctcagcgagc tggaaaacga
     2641 aatctaccaa ttaccgcccc gctctgctta acaactctct tttgcgcctt ctgcaaccat
     2701 catgttgcag aaggccttgt ttttcatgga atacaacatg tctatcagct cagtaatcaa
     2761 atcattacaa gatattatgc gcaaagatgc cggtgtggac ggcgatgcgc agcgtctcgg
     2821 tcagctctcc tggctgctgt ttttgaaaat cttcgatgcc caggaagagg cgctggaact
     2881 ggagcaggat aactatcaat atccgatccc acagcgttat ttgtggcgca gttgggccgc
     2941 caacgcgcag ggcattaccg gtgattccct gctggaattc gttaatgatg atctgtttcc
     3001 ggcgttgaaa aacctcactg cgcctatcga taaaaaccca cgcggctacg tggtgaagca
     3061 ggcgttcagc gatgcctata actatatgaa aaacggtacg ctactgcgcc aggtgatcaa
     3121 caagctgaac gaaattgact ttaccagcgc cagcgaacgc catctgtttg gtgatattta
     3181 cgaacagatc cttaaagatc tgcaatctgc gggcaatgcg ggcgaattct atactccacg
     3241 cgccgtcact cgctttatgg tggatcgcgt tgatccgaaa ctcggcgaat ccattatgga
     3301 cccggcctgc ggtacgggcg gttttcttgc ctgcgcgttc gatcatgtaa agaacaaata
     3361 cgtgaagagc gtcgccgatc atcagacact gcaacaacag atccacggtg ttgagaaaaa
     3421 acagcttccg cacctgctgg cgaccaccaa tatgctgctg cacggcattg aagtaccggt
     3481 acaaattcgt cacgacaaca ccctgaataa accgctttcc tcctgggatg agcaactgga
     3541 tgtcattgtt accaacccgc cgtttggtgg cacggaagaa gacggtattg agaagaactt
     3601 tccggcagag atgcaaaccc gcgaaacggc ggatctgttc ctgcaactga ttgtggaagt
     3661 acttgcgaaa aacggtcgtg cggcggtggt attgccggat ggcacactat ttggcgaagg
     3721 tgttaaaacc aaaatcaaaa agctacttac cgaagagtgc aacctgcata ccatcgtgcg
     3781 tttaccgaat ggtgtgttta atccctatac cggcattaaa accaacctgt tgttctttac
     3841 caaaggtcag ccaaccaaag agatttggtt ctatgaacat ccgtatccgg cgggcgtgaa
     3901 aaactacagc aaaaccaagc cgatgaagtt tgaagagttt caggcggaga tcgactggtg
     3961 gggtaacgag gccgatggtt ttgccagccg cgtcgagaat gagcaagcgt ggaaagtcag
     4021 cattgatgac gtgattgcac gcaacttcaa tctggatatt aaaaacccac atcaggcgga
     4081 aaccgtcagc catgatccgg acgaactgtt agcgcagtat gcaaaacagc aggcggagat
     4141 ccagacgctg cgtaatcaac tgcgcgatat tcttggcgct gcgctgtctg gcaaggaggt
     4201 taactaatga gtgtggaaaa gctgatcgtt gatcatatgg aaacctggac ctcggcgttg
     4261 caaacccgtt ccaccgccgg gcgcggcagc tccggtaaga ttgatttgta tggtattaag
     4321 aaattgcgtg agctgattct ggaactggcg gtgcgcggta aactggtgcc gcaggatccg
     4381 aatgatgaac cggcgtcgga cctgctgaag cgtattgcgg cggaaaaagc agagctggtg
     4441 aagcaaggca aaattaaaaa gcaaaaacca ctgccagaaa ttagtgagga agagaagccg
     4501 tttgaattgc cggagggatg ggagtgggtg cacctccctg atatttattg ctcaatttct
     4561 gagtcatcta gaaaaataaa atcctccgaa atactacctg agggaaaata tcctgttatc
     4621 gagcaaagcc aagaatttat ttctggttat tgcaacaatg aatgtctgtt aataaagtta
     4681 aataatccag ttatagtttt tggtgatcac acaaggaata ttaaatttat tgattttgat
     4741 tttgttgtcg gcgctgacgg tgtgaaaatt ttatcaccta tattaatttg tgagcgtttc
     4801 tttttttggc agttacgatc atttaaatta gatgtaagag gatacgcgag acattttaaa
     4861 gtattaaata gctgcttatt tgcattaccc cccattgcag aacaagagcg aatagttgaa
     4921 aaagttagct ctttgatgtc cctctgcgac caactagaac agcaatccct gaccagtctg
     4981 gacgcacatc agcaactggt tgaaaccctg ttgggaacgc ttacagacag ccaaaacacc
     5041 gcggaactgg ctgaaaactg ggcgcgtatt agcgagcatt tcgacaccct atttaccacc
     5101 gaagccagcg tggatgcgtt aaaacagacc attctgcaac tggccgtaat gggcaaactt
     5161 gtgccgcagg atccgaatga cgaacccgcc tctgaactgc tcaaacgtat tgcgcaggaa
     5221 aaagctcaat tggtgaaaga agggaaaata aaaaaacaaa aaccactgcc gccaattagc
     5281 gatgaggaaa aaccgtttga acttccggaa gggtgggaat ggtgtcgtat tggtaatata
     5341 gttaatatta aatctgaatt agtatcacca aaagattatt taaatttata tcaagtagca
     5401 cctgatatta ttgaaaaggg cactgggaga gtgatatcaa agagaacagt gaaagaatca
     5461 ggagtcaaag ggccaaatag tcgcttttat aaaggacaaa ttgtttattc taaaatccgc
     5521 ccgtcattat ccaaagtttt tttagcagag tataatggtc tttgcagtgc tgatatgtat
     5581 ccattagact gttacattaa tccaaattac ttattgaaat atatcctttc cattccattt
     5641 cttatgcaag tcaaaaaagc agagaacaga ataaaaatgc cgaaacttaa ttctgacagt
     5701 ttttacaata ttattgttgc gattcccccc tataatgaac aacaagcaat atttgataag
     5761 ataaattcta ttgaagcagt atgcaatggt cttatttctt atattgggat ttatcataaa
     5821 acccaactgc atcttgcaga cgcactcact gacgcagcga taaactaatt ccctcgccct
     5881 cacaaaacca ataaagcggc taaatagccg ctttattcac atcaccaaaa tttatatttc
     5941 acgctaattt tcatcttatt ttcattaatt tttagctgga ctttccctgt atttactgat
     6001 gatatatcca ggtatttagc acggtgcgga tgtgcgccaa cacactcgca ccgctaatca
     6061 caatcactat ttatagagaa tagtaatcat gactgacacg cattctattg cacaaccgtt
     6121 agaagcagaa gtctccccgg caaataatcg tcaattaacc gtgagttatg cgagtcgtta
     6181 cccggattac agccgtattc ccgccatcac cctgaaaggt cagtggctgg aagccgccgg
     6241 ttttgccacc ggcacggcgg tagatgtcaa agtgatggaa ggctgtattg tcctcaccgc
     6301 ccaacccgcc gccgccgaag agagcgaact gatgcagtca ctacgcaagg tgtgtaagct
     6361 gtcggcgcgt aaacaaaggc aggtacagga atttattggg gtaattacag gtaaacagaa
     6421 agtcgcctga tacattgacc cggtagcgcc agtgctaccg ggtattattc gccgcgttat
     6481 accaacgcgc tctccatctg tttttcaaat gcctgacgaa ttaatcccat aatgtaagga
     6541 agttgtgcga ggtcgctgaa accaatttct acatcgccat taccccagcg cccaacattt
     6601 gtcacatctt tggcaatacc tttcggatcg accagttcat gaaactgcat attaagcgac
     6661 aatcgcagtc ggcttttttg cggcacaaca tcaacaaaat tcgtttccgc tttaaacgca
     6721 atatatagct tcaatacttc ctgcgtgatc cctgcgtcca ggcgc
//
LOCUS       DAJTYG010000156         6508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTYG010000156 DAJTYG010000000
VERSION     DAJTYG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.156"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..173
                     /locus_tag="OO568_003703"
                     /pseudo
     CDS             1..173
                     /locus_tag="OO568_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            750..1878
                     /gene="ltrA"
                     /locus_tag="OO568_003704"
                     /pseudo
     CDS             750..1878
                     /gene="ltrA"
                     /locus_tag="OO568_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            1961..2140
                     /locus_tag="OO568_003705"
                     /pseudo
     CDS             1961..2140
                     /locus_tag="OO568_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2170..2652)
                     /locus_tag="OO568_003706"
                     /pseudo
     CDS             complement(2170..2652)
                     /locus_tag="OO568_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2908..3888)
                     /locus_tag="OO568_003707"
     CDS             complement(2908..3888)
                     /locus_tag="OO568_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6642861.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3888..5087)
                     /locus_tag="OO568_003708"
     CDS             complement(3888..5087)
                     /locus_tag="OO568_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6642862.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5409..5747)
                     /locus_tag="OO568_003709"
                     /pseudo
     CDS             complement(5409..5747)
                     /locus_tag="OO568_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5814..6508)
                     /gene="istB"
                     /locus_tag="OO568_003710"
                     /pseudo
     CDS             complement(5814..6508)
                     /gene="istB"
                     /locus_tag="OO568_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
BASE COUNT         1736 a         1506 c         1555 g         1711 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtggagg tgggccctcc ggtcagggtt tacaggagca
      241 ctgaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtggggttc gggaagctga acgaaagtaa actgtccgat
      361 gaagcatcga aatcttagat gatgccaaaa tggggccgta cagccgtccc ctgataagcg
      421 cagaagacac ctgcttactg actgcgcggc atccggtgtg aaggcagcag gatcccgggc
      481 cagaccatct cagggaacgt gagaacctgt ttccggcacg tatcgggaaa caggggcgga
      541 cccattcgta gtagtgatga aagatctgtc atgggtctgg agcgaaggga tggggatcac
      601 attcattgca gaagacacaa cccaaaaggg gatgacgtaa ctgatgcaac gaaaatcatt
      661 tgaaatacca aaagcactgg tctgggcatc atatctggat gtgcgtcgca acaagggagc
      721 cccccggatg cgacgggcag actttgaaaa tgttcgacca gcaacgtgat ggcaatctat
      781 acaagatctg gaaccggcta tgttcgggaa catggtttcc gccaccagtg ctggaaaaac
      841 ggatcccgaa gtcgaatggc aaggaacgca ttctggggat cccaacagta tccgatcgaa
      901 ttgctcaggg agcgataaaa cttttcatgg aagaaaagct tgacccgatt ttccatgctg
      961 attcatatgg ttaccgacca ggcaaatcag ctcatgacgc tctgaaacaa tgtgccatcc
     1021 ggtgctggcg ttatagctgg atactggaag tggacatcag tgcgtttttc gaccatgtga
     1081 gacatgatct ggtgctcaaa gctctggaac accacgggat gcctaaatgg gtcatcctgt
     1141 actgtcgacg ctggatggaa gcgccaatgc agagttgtga aaatggagaa ttaataaccc
     1201 gaacacgagg cactccgcag ggcggggtca ttagtccgct acttgctaac ttgttccttc
     1261 actatgcatt tgatttgtgg atggaaagag aatatcgggg ggtaccgttt gagaggtacg
     1321 ctgacgatat tgtagtacat tgttcacgaa tgagtgatgt aacaaggcta aagaacaggt
     1381 tatcggagcg cttctcggaa gtcgggctgg tcctgaatgc agggaaaacg aatatcgcct
     1441 acattgacac gtttaaaagg agaaacgtcg caacgagttt cacctttctc ggatatgact
     1501 tcaaagtgcg tacgctgaag aatttcaaag gcgaacggta ccgaaaatgc atgccgggtg
     1561 cgtcaaatgc agcaatgctg caaaataaca gaaacaatca agaagtggcg tatacatcgc
     1621 tcaacagctg agagtttgct ggattttgcg aggcgctaca atgcgatagt gagaggctgg
     1681 atcgagtact acggaaagtt ctggtccaga aatttcaact atcgactgtg gagtgcaatg
     1741 cagtcacgtc tgctcaagtg gatgcagtct aaatacagac tttcgaaccg gaaggctcag
     1801 cgaaagctga cgctggtaag gaaggagtat ccgaagctat ttgttcactg gtatttacta
     1861 cgtgcatcaa atgagtgatc aagagccgta tgacgcgaga gtgtcacgta cggttccgtg
     1921 agcagccggg gagtgaaatt ctctccggct gactcgactc gcgggttccg acagcgatga
     1981 cgaacatgcg gcggtgtttt actcgctgat cggcacatgc cgtctgaaca atgtggaacc
     2041 agaaaaatgg ctgcgttacg tcattgagca tatccaggac tggccggcaa accgggtacg
     2101 tgatctgttg ccctggaaag ttgatctgag ctctcagtaa atatcaatac ggttctgacg
     2161 agccgcttac ttttggcgta attctgctgc gcctttactg aacacaatgc cgtattcagc
     2221 aagaagtcca cgaatgtgat ttatcagttg tgtcctttcc attgaaacca accgacgcat
     2281 attcaaaaaa gtctggacgt cctgttgctc cagagttttt attggtacgg gtcgtatagt
     2341 tccatgttgg caggcctcag ctattgcctg tgcgtcgtta taatcatttt tctggcaccg
     2401 ctgataagct ttaacatgtt gtgccggaag caatattaca tcatggtcga gtttcctgat
     2461 ttcccgggcc cagtaatgag atgccccgcc agcctccatc gcaacagtca ttttaggctg
     2521 ttgcacctgc cagttaagca gttgattgcg agaaagtttg gtctttcctg cgggtttgcc
     2581 atgagcattg atactgaaaa gataaaagtt cgttttagcc agatcaatgc cggtgatttt
     2641 ctgttcagac atggtatccc gcttactttt gtggttaaag tttatccatc atggcgcaat
     2701 tgaaggctgc cgtgaaagag ggggaagact atctcatcgg ttactgttaa acggctaaga
     2761 gaaactatct ttttcaagca aagaagatac ggtgaaacag gcgatttcac cgtgaaatac
     2821 tgttcaaaaa aaacgctaat aaaagcaaaa tttcttttaa aacagccaat taaaaactgg
     2881 tgagttgctg gcgaaaaact tgaattttca cagcgaattc agattatctt ttagtacaag
     2941 agcaatcata cgatctaact tatcctgaac ttccaacggt aatctgccga attcatatga
     3001 gaacgtacgt ccctttactc gttttcgagc aaatttatct ttactatcaa atttccataa
     3061 caaagttaca accgctttat cctttactcc tgaatcaagc aatgaagaca tttccttggt
     3121 tataagccgc ataatactgt ttttaacctc gtcttcagaa agattgtcat cagcagtaat
     3181 aagctcaata ctcggcgaga tattcttcac aacttcatca atagatatat cacccagcct
     3241 cagtcgttca cttaattcag ccagttgcct gtaatcggcg tatgtcagct cagactgaac
     3301 aggaaaaaga gacacaaaat ctttaggtac ggacgctgct tggagggcac gagtcacctt
     3361 agctgccgac aatccctctc tttccgcaat ttgcttctgg ttcataccgg cctctttgag
     3421 acgtactaaa cgcaacccaa tttcacgaat attatgttca agtgacgtct gcaaatcttt
     3481 cgccagatgc tgagcttcag aaacagtaag ctcatcatcc gttacaagta cacgtaatcc
     3541 aaccttgcat agcaaagctg ctgcccgacg acgggaacca tccaaaatct caataagatc
     3601 gccagtccga atcccaatac atggatagaa ttgttgtaaa cgtatagttc tggtgatgtc
     3661 ttttagtgag tccggcgtca gggctgtctg ttcgcggcca ttcacctctt caaccacaaa
     3721 agtttgagtg tccactttgt catgatcaac cttgatctca gtaaattttg cctttcggcc
     3781 agtattgagg gtaaatacat gaactggagc cgatatttct tcagtattat tcaggatatt
     3841 tgtattcagc gtacggccaa tagtcggtcg gtgctttctg ttttccatca gtagttggcc
     3901 cttataaatt caatacgatc aaaaactgct ttagcgaaat cttctgctgc catacgtgca
     3961 tttttcaaag cttcaccact gccaacataa gtaacaggat tcgcagatat cacggtatca
     4021 aacgactcgc cgcttctctc aaaaccatca agacgtggca ttgaaacatc aagcatgtca
     4081 ccaccgaaaa tctcttttgt caggctatgg cagtatttat gatctgactt attcgccagt
     4141 ttcgacatga agccaatatt tgcctgcaaa cggcatgaac atccatcctg ttcaataatc
     4201 tgtacaagtt caggtaaacg agccaaatat ttaagcgtgg aatggaaatc aacttgtgct
     4261 ggcggcaccg gtgtaaacat gatatctgcg gcagcaatgg catttttcaa aaatgcgtcc
     4321 agatgcgggc ctgtatcaat aaggatgaag tcgaaatcat gcttcagctt atcaataata
     4381 ttctcacgca atatcgcgtg tttgttttgt cctaatagat gctcctcgca cagagtatcc
     4441 cagtttgatg caataaaggc gtcgtcaata gaagccggca taacataaac gccagggata
     4501 actgacggaa cgataaaatc ttccagtaat tcctcacgac tcacgttctg caacatagcc
     4561 tgcggtgcgg ttgtatcaac cagccccacc gcatgcaagt agttcagaaa catagtggct
     4621 gatgactggg gatctaaatc aaggagcaga atacgtaaat cttctgacag caggtgagga
     4681 tgagcacgca gggcgtgagc tacggaaaca ctactcacgg ttttagacac accgcctttt
     4741 agactaccga tgaatatgga atatgcctca ctataacgat cgcgatattt aggaatgcca
     4801 cgatgtgcat agatatcgat aatgttctgg atggttaatg catattttgt cgcggttcct
     4861 gctgggcgtt tttcaaactg atagccctga gcctccatct caccgactgc accatccacg
     4921 cttgcgcgcg ttaattttgg aagcttagct acagcagact ttgaatagac ttgataaaaa
     4981 acatcagcct gaaactcctg cttttgctcc tgaatctgtt ttgttagggc gagcaacatt
     5041 ttatctgcac gctgcgcaac cttacttaac tgctcaaaac tagtcattcc ccctccgtgc
     5101 tatgtaggat tttaacttat tacaaaaaaa actgcattag cagggtaatg catgcaagta
     5161 catattgtac atgcttatat taaaaatgcg cgcattgctg atttattaat catcggaatg
     5221 aatattactg taagatagta tgcctttccg ggagaaagac gctgatttcc cccgaataat
     5281 ccagttcacc accacactcc ggacaacagc tttcttcagg ctccagatgg tgtatttcgc
     5341 gggaaacatg ttccggtaac ggacggcgat gacgtgactg tcgcaacagg cgggggtagt
     5401 gcttccaatc agtagaacat gtgcttttca atgaaggagc cgatgacttt ttcgtggatt
     5461 tccacggaac gcgagaagca gattgtttta cgtgccagcc gcttgatgcg ggtgcggagg
     5521 gtccggttgt tacgttcaat acgttgcgtg aagattttgc cggtcaggtg tttctccttc
     5581 ggcacttctc tggcgtagct gccccagtca tcgctggtca ccatgccgat acagaacgga
     5641 gtcaacaggg ctaacagttc ccgacaggtc tcatcattcc gtggcccgaa agtgtaagcc
     5701 aacacaccgc cagttttagt gttgtacgca taccagagcc agtgttgtgt caacgacgga
     5761 tgaaaagtgc tccacttata tctccaccaa cggcccaata ttgatccacc gttttactca
     5821 ggattagctt ctgctataac cccggccttt cgtttctgtc tgagtcgata gctttctcct
     5881 ttgatttgaa cgacatgtga gtggtgtaag atacggccca gcatcgctga ggtcagtgct
     5941 gcatcaccgg cgaacgtttg atcccactgc ccgaacggta gattggatgt caggatcatt
     6001 gcgctctttt cgtaacgttt agcgatgacc tggaagaaca gttttgcttc ttcctgactg
     6061 aacggcagat agcctatttc atcaatgatg agcaggcggg ggccattact ccacgctgaa
     6121 gcgtcgtttt ataacggccc tgacgttgtg ccgtagataa ctgaagtaac agatctgctg
     6181 ctgttgtgaa gcgaactttg atacctgcac ggactgcttc atagcccatc gctattgcca
     6241 gatgggtttt ccccacacct gatggcccca gtaatacgat attttcatta cgttctatga
     6301 cgctgagtga gcgtaacgac tggagttgct tctgcggtgc tccggtggcg aatgtgaagt
     6361 catactcttc gaacgttttc accgccggga aggctgccat tcgggtatac atcgcctgtt
     6421 tacgttgatg acgtgccagt ttttcttcat gaagcagatg ctccaggaag tccatataac
     6481 tccattcctg gtctactgcc tgttgtga
//
LOCUS       DAJTYG010000157         6490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTYG010000157 DAJTYG010000000
VERSION     DAJTYG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.157"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..920)
                     /gene="rspA"
                     /locus_tag="OO568_003711"
     CDS             complement(<1..920)
                     /gene="rspA"
                     /locus_tag="OO568_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR6642863.1"
                     /translation="MHCERKTYQAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRE
                     LSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKA
                     KAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMK
                     TTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMH
                     HRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDC
                     KQLIEEQLIDYIRTTLTHAGGITGMRRIAD"
     gene            962..1303
                     /locus_tag="OO568_003712"
     CDS             962..1303
                     /locus_tag="OO568_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR6642864.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1369..1898
                     /gene="speG"
                     /locus_tag="OO568_003713"
                     /pseudo
     CDS             1369..1898
                     /gene="speG"
                     /locus_tag="OO568_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460462.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(1901..2611)
                     /locus_tag="OO568_003714"
     CDS             complement(1901..2611)
                     /locus_tag="OO568_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR6642865.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2719..3024
                     /locus_tag="OO568_003715"
     CDS             2719..3024
                     /locus_tag="OO568_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR6642866.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3223..3546
                     /locus_tag="OO568_003716"
                     /pseudo
     CDS             3223..3546
                     /locus_tag="OO568_003716"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4102..4701
                     /locus_tag="OO568_003717"
                     /pseudo
     CDS             4102..4701
                     /locus_tag="OO568_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(5279..6268)
                     /locus_tag="OO568_003718"
     CDS             complement(5279..6268)
                     /locus_tag="OO568_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642867.1"
                     /translation="MTMIKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGERPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTVKLDTGAATAVLPVVKRLKFTKTEA"
     gene            complement(6343..>6490)
                     /locus_tag="OO568_003719"
     CDS             complement(6343..>6490)
                     /locus_tag="OO568_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642868.1"
                     /translation="KELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADIESGGNW
                     MDSL"
BASE COUNT         1665 a         1643 c         1598 g         1584 t
ORIGIN      
        1 aaatcggcaa tccggcgcat accggtaatt ccgcctgcat gggtcagcgt ggtgcggata
       61 taatcgatga gttgctcttc aatcagttgt ttgcagtccc agatgctgtt gaagacttca
      121 cccactgcga tgggtgtgac ggtatgttgg cgaatgagac ggaagcattc ctggttttcc
      181 gctggcgtcg ggtcttccat ccagaacatg cgataatctt caatgctttt accaaagcgc
      241 gccgcttcaa taggcgttaa gcgatggtgc atgtcatgca gcaaatgttc attaaaacca
      301 aacttgttac gtaccgcgtc aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac
      361 cacagctgct cttccggcca ctgtcctttg gttgcgggtt cataagccag acctttacct
      421 ttcgacatgc cgtaggtggt tttcatacca gggattccgc actgcacgcg gatggctttg
      481 aatcccagct cctgatgacg ggcataatca tccagagctt catcaatact gtgaccggtg
      541 gtatggcaat aaaccatcac cccttcacga gacgcgccgc cgagtaactg gtaaagcggc
      601 atgttggcag ctttggcttt aatatcccac agcgccatat caaccgctga aatggccgac
      661 atcgtaaccg gaccgcgacg ccagtaagca cctttataga aaaactgcca gatatcttcg
      721 atacggtgcg catcgcgacc aataagccgc ggacaaagat gatcctgcaa ataagaggcc
      781 acggaaagct cacgtccatt gagggtggca tccccaaggc ccgtaatacc gtcctcagtg
      841 gtgattttta atgtgacgaa attacgcccc ggacaggtaa caaaaacttc agcctggtac
      901 gttttgcgtt cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat
      961 tatgaaaatc actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact
     1021 ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag
     1081 tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct
     1141 gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga
     1201 taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt
     1261 gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag
     1321 gaatgtaagg acacgttatg ccaagcgccc acagttaagc tacgcccgct ggagcgtgaa
     1381 gatttacgct atgtacatca actcgacaat aacgcaagtg tgatgcgtta ctggtttgag
     1441 gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcgcat attcacgatc
     1501 agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg
     1561 tggaaattaa ccatgttcat cgccgggcag aatttcagat aattatctcc ccggagtatc
     1621 aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc
     1681 tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt
     1741 accgcaagct tggctttacg gttgaaggtg aattgatgca tgagtttttt attaatggtc
     1801 aatatcgtaa tgccattcgc atgtgtatat tctagcatca gtatctggca gagcacaaaa
     1861 caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa tcgatcgtat
     1921 ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgatcagct
     1981 gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaactc
     2041 gttgattatt tagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg
     2101 gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc
     2161 cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta atcacgaagc
     2221 cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc
     2281 cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg gcgtccagta
     2341 ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt
     2401 cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct
     2461 gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg
     2521 ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg
     2581 cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag agatgattct
     2641 caatcatagc agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct
     2701 tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc
     2761 gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca
     2821 cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat
     2881 gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa
     2941 attctctaca accgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat
     3001 ggcgcgcctg ccgaaccgca gtaacacatt atcgactgaa cgccggatat gacacatccg
     3061 gcgatttgaa catacaacat aatctcacct tattactcat acccttctat tgatgtggat
     3121 taataattct taacccaaaa tgggtagact ccctctattg ttagcgcgct aaatattcaa
     3181 tatataaaca tttatataac gataaagaac agggagtgag ttatgtccaa aaatgaccga
     3241 atggtgggca tcagccgcag aacactcgtt aaatcaaccg cgataggttc tctggcgctg
     3301 gctgcaggcg gtttttcttt accgttcacc ctgcgcagtg cagcggcagc ggtacaacag
     3361 gccagcgaaa aagtgatctg gggttcctgt tccgtcaact gtggtagccg ctgtgcactt
     3421 cgtctacatg ttaaagataa tgaagtgacc tgggtggaaa ctgacaatac cggcagcgat
     3481 gagtacggca accatcaggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt
     3541 aatcatcccg atcgcttgaa ttacccaatg aaacgcgtgg gtaaacgcgg tgaaggcaaa
     3601 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
     3661 gtcgaacagt atggcaatga agttgtatat attcagtact cttcggggat tgttggcggc
     3721 aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc
     3781 ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac
     3841 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
     3901 atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt
     3961 gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat
     4021 acggctgccg gtcgtgaaga cgaatggctc cctattcgcc cgggcaccga tgccgcgctg
     4081 gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat
     4141 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
     4201 tataaagcct atattcttgg tgaaggtgac gataaaacag caaaaacgcc gcagtgggct
     4261 tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
     4321 acaaaacccg cttatatctg ctagggctgg gggccgcaac gccaggcaaa cggcgagctg
     4381 actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga
     4441 aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat
     4501 aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg
     4561 caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag
     4621 ttcatctgga actacaagga cttgaagcgg ttactcgtta gagtcacgag agaccttagt
     4681 aggcctgagg tcctgcattg agtcaagacc ttgtgtcatt ctttgtgcca gacttgtgtc
     4741 attagacggg atgtctagac ggctggtcag gacccccggt ggcctctttg tcaggactca
     4801 gggtggcacc ggggaacatt ggtgtcttaa ttgtattgaa tgaggtctct aacttttggt
     4861 ccaaaactca tcgttagctg ctcgccagct cacagccgaa cccgaactgt aagcacgacc
     4921 accagcacca gcttcagcag gaagacgaca agatagactt tatgcaggtc catgcgccct
     4981 cctgtgtcta ttgtaccact gcactagata actagtacaa aataaagtcc agaccgaagg
     5041 cccagaccat agttaattaa tcacattgat aatccaccac ctgaagtcct gccaacattc
     5101 agtccagcct tacccatgct aggagtctta accggggtgt cgttctgtgt ctccaccact
     5161 ggtgccgcat caggctctac aggaagctcc acagcaggcg cagggaccgg aggtgatacg
     5221 gatgagaagc ccttagcgat ggaccggagg cctttactaa tgctcttacc cattgctgtt
     5281 acgcttcggt cttggtgaac ttcaggcgtt tgactacagg caggactgcg gttgcagcgc
     5341 cagtgtccag cttaactgtc gtagcagctt cagcgacaac gaagacctta ccgaggattt
     5401 ccaagtcgtg ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt
     5461 cgtgacggaa gcagccgaag ctcagggccg tgaagcgttt agccagcatg aacacacctt
     5521 tgtcctcacc gttgtaatac gcgatgtcgg tacccagcat atccacgata gcgtcttcac
     5581 cagagaagcc ctgtttagca gccagacggg acagcgcttt cagttgggac tcatgggcga
     5641 tgatgccgta atcagacact tcagagccga aagaggactc ggtgttctca aacagagcgt
     5701 ccatgatagc gtctgcctta gtggtcaggc ttccagtcag gactggcagc tctacggtct
     5761 tcagcagctt ggaggactcc agaacgtcaa ccatgcgagc gttgatggta cggaccacat
     5821 tctcacagat gttcatagcg tcctgctgga gtgtgccatg agtgaaacca gtggaggact
     5881 tggcgtccca cagtttagtt tgctccgaga tgaggattgc ctgttcgata ggttcgacga
     5941 caatctcctg accagacgga acggtcacga cttcaggtcg ctcaccaccg cgtacaggga
     6001 cgaccacaga gcggtcagac agggtgtggg cgatgattgg ggtaggcagg ctattgcggg
     6061 taggcaggac ttctacaggg aacggaatgt tatcctgagc gtctttaagt acaccgttca
     6121 gcatagcctt aaccgtctta gctaactgga gggcatagac ctgtgggtca gcgttagttg
     6181 gcagaccttc gttaacctga atgttgtcgg tgtcggtgat acgtactggc tcaatgccga
     6241 actgatagct atctttaatc atggtcataa taatcaatct cctaaattag ttttagatac
     6301 tgtgggtccc gacatcaggt caggaccact tgtgtgtctg tcttagaggc tgtccatcca
     6361 gttaccgcct gactcaatgt cagcgacaat agcggcacgg gagcgaccag cggaatgggt
     6421 agcactgcgg aggttctcac cataggtccg gcgctcagct ttgacctgtt cgcggcgctg
     6481 tagctctttc
//
LOCUS       DAJTYG010000158         6241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTYG010000158 DAJTYG010000000
VERSION     DAJTYG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.159"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..312
                     /locus_tag="OO568_003720"
     CDS             <1..312
                     /locus_tag="OO568_003720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6642869.1"
                     /translation="VAMIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKT
                     HDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNV
                     R"
     gene            complement(388..852)
                     /locus_tag="OO568_003721"
     CDS             complement(388..852)
                     /locus_tag="OO568_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6642870.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(849..1724)
                     /gene="nanK"
                     /locus_tag="OO568_003722"
     CDS             complement(849..1724)
                     /gene="nanK"
                     /locus_tag="OO568_003722"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6642871.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEATASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1721..2410)
                     /locus_tag="OO568_003723"
     CDS             complement(1721..2410)
                     /locus_tag="OO568_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6642872.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="OO568_003724"
     CDS             complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="OO568_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6642873.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTHKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="OO568_003725"
     CDS             complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="OO568_003725"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6642874.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="OO568_003726"
     CDS             complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="OO568_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6642875.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1579 a         1690 c         1550 g         1422 t
ORIGIN      
        1 gttgccatga tttacattga acagcttgaa ttaatccata aaagtggtga tgtgttgtat
       61 ccagtaaaaa taaccagaaa atcatcagga aaaactgcct tccaccttgt tccattcggc
      121 ttaaataaga ctcacgacct tctagaggtg gaagatgctt cagaagcaat tcggctggtg
      181 attgatgaac gtcattccat tcgctgcagt acacttacag caactattac gaataaaaag
      241 ggcaagcgaa ttaagcggac aggtatatac agtatcaagg gtgttaacat aaaagaatac
      301 aacgtcaggt aatggcacag agcggtccgt cagttcaggt ttggctctgt gccaaagatg
      361 ggtcaggtca cacctgatct aataaattta agccattaaa tcagccttaa ccttcaccac
      421 caccttttta atctcacccg gctcgccaac aacgcatccc ggcttatgcg gctctcctgg
      481 cataaacacg gcgaacattc ccggtttcag gatgatggtt tgctcgtttt caatggcgct
      541 acaaagctgg taatcatctt catggtggaa ctcttcacat tggcgcgcag tgcctgccat
      601 gccaaacaga atccgttcct caccgtttaa taacagctgg atatcaatgt attgctcgtg
      661 caattccgct tttttctcga cgggcgattg agtgttaaac gtcatgacat tcataaaaat
      721 attgtcgccc tgtaattcgt aacgacccgg cgctttttct tgcggtctgg cagctaaggc
      781 cagcgttaac gcgtcctgta acgcaggatg taacccagca gacggtaatg actgtacttc
      841 acccatcatc ataatttttc tccctgggcc aacagcgcag ccccaagtaa acctgcatca
      901 tggcggtaat gcgccgccag taaatcaaca tgaaatgccg ctggctcctg cgccagatac
      961 atttccacca acgccagata cccttctgcc agaccaacgc tgccaccaac caccacgcac
     1021 tggcaatcag ttgtggcttt aatatcagcg atcagccttg caagcgtacg tgcggagcga
     1081 tgaatcagtt gctgcgcctg ctcatcgccc tgcccggcgc gtgtgaaaat agttctcgca
     1141 tccgcgccag ccagctcgcc ctgcgctgcc gccgcaatgc cgcgaccaga agcagttgct
     1201 tccacgcaac ctgtgcgtcc acagccgcag actgggccgt gtggatcggc aagcgtatgc
     1261 ccgatgtgcc ccgccagacc gccagggccg gtaagcagtt tgccgccgct cactacaccg
     1321 ccgccgacgc cagtggaaac ggtgataaag accatatcgg ttatatcgcc ttccagcgcc
     1381 tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg caatggtcgg caaattggta
     1441 agttgttcca gcgttttgac taacggaaag tgtagcaatc caccgagatt atgcggattc
     1501 agcgccagca aactgccgtc gcgaataatt ccggttgaag cgatggcaac ccgctgcgca
     1561 tgagcttgca acggagagac taatgcggat aaggcatcac gcaaggcttc tggtgtctgg
     1621 ctggctggcg taggaagttc acgacgatcg cggatctgcc cgtcagcgcc aatcagcgcg
     1681 gcggcaagtt tagtaccgcc gatatcaatc gccagtatgg tcatagcacc gcctttttca
     1741 tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt aatggcagaa ccgaccgtca
     1801 ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc aggcgtgttg taacgccctt
     1861 cggcaatcac ccgacatccg gcttcgctca acgttttcac cagcgccaga tccggctctt
     1921 ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc aataatttcg gctcccagct
     1981 tttggcatgc caggccgtct tccggcgttg agcagtcggt catcgccagt aaaccgtgat
     2041 gatggatacg tgccagcagc gtttcaacag gcaccggacg cgggcggtcg gtgccgtcaa
     2101 tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac atcttcaata taggccgtga
     2161 tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc aataatcggc acgctcacca
     2221 ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg aatggcaacc gcgcccgcct
     2281 gctctgccgc taatgccatg gctgcgacga tttcgggttt atcgagcggg ctgtcaggaa
     2341 ccggctggca ggagacaatc aggccaccgt tagcagcgat tttttgatcc agttgtgcaa
     2401 gtaacgacat acatcttccc ttagcgaaag gcccggtaca tagaccgggc aacaggatta
     2461 acttttggtt ttgactaaat cgtttttggc gctgccaaat ggcacggcac cgctaaatgg
     2521 tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg cgcagccaac gctgaacgcg
     2581 ggaaggcata tccagaccaa tcagcaggat caccacaaat gtcaggctga acgagagcga
     2641 tgccagcgca gtacccagat ccagacgttg agcgatcaac gcgccgatga ttggggccag
     2701 tgcgccaccc aatgcgccaa cgttgtaggt aaagcccagg cccgctgcac gctggtcggt
     2761 atcgaaataa ccgccaatca gtttcggtaa gatcccggcg atcccttgtc caagcatttg
     2821 ctggaagaac agtaacagac cgagcaccca gacgtttgcg ccgccaatcg caaataccgg
     2881 aataatcagc agctgcgagg ccagcaggct acaaacgtac gctttgtggg ttcccagcca
     2941 gtcaccgagg aagccaccta cgcagcatcc cacggccgcg ccaaagccac taaagaacag
     3001 cacattggcg acagtatgcg ggttataagc cagatcggtt ttcagatacg ttggcagcag
     3061 cgcctgaatc ggccatgagt agaggaaagc aaacaacacg accaccatca gcattacgcc
     3121 cgttggccag cgtttacccg tactttgcac cataaagctg ataaagattg cggcgcataa
     3181 cagcccgaga acagcgacga ttgcagcatt ttgcaggtta ccggcgaagc agaaccacag
     3241 cgcagtagcc gctgccagtg tcattacgat attggcaatg cgatgttcac cacggtagag
     3301 aatatccacc attgtgcgta ctggtgcttt acctgcgtgt ttctctttcc agtcttccgc
     3361 ttccgggatg tttttacgca gccagagagc aaagatgatt ggcaaaatgc cgataaagaa
     3421 cagcgcacgc caaccccaga ccggaaccac caggctatag acctgagcgg caacgacggc
     3481 ccccacagag aagcctgaaa tcaaaaaacc actggctttg ttacgcagat gttttggcca
     3541 gctttcaatg acataggtgg cgctggaacc gtattcaccc gccatcccca tgccgatgac
     3601 cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag ccgcaggcca gcgtcccggc
     3661 cgagaagaga acgatgctgg tgaccattgc cagacgacgc ccgtagcggt cacccatagc
     3721 gccgagcatc aggccgccga accagcgaga gataaaggct gcggagatca gacttgccgc
     3781 ctgcaccgtc gtcagcccga attcaccctg tacttcggtg agtaccaggg cgattaaaac
     3841 gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg gaaaatgcgc gccattgtgc
     3901 acggttgaga tggcgatacc acgggatatt ctgggttgta gtactcattg tgagtctccc
     3961 gcggtgggcg atgcccacac gctttggtat gaaaattgta gggtacagat gcgtttattt
     4021 cccctcaccc ggtaggggcg agcgagggga aaacaactca cccgcgctct tgcatcaact
     4081 gctgggccag cgccttcagt tctggcagat atttttcatc taccggtcca aacggtttgc
     4141 ggcacagcgg cacagaaacg acatccatat aatgcaggac agttttcagg ccgcggaata
     4201 cgcccgtttt gatcagtaaa tcaatgactt tgttgcactc agtttgcagt ttctgcgcgg
     4261 tctggatatc gccttctttc agcgccttca cgatcccctg atatcgccag cccatgatgt
     4321 tgtaagtact gccgatacca ccatcagcgc ctgccagcaa gccggaagcg aagatttcgt
     4381 cgtaaccgtt atagagcacc agatcaggat gttcacgacg gatctgttcc atctgataga
     4441 gatcgccaga agtctgtttc agcgcaccga cgccaggcaa cgtaaccagg gtgttgatct
     4501 gatccagggt cagttttacc ccactcaggg ctggaatgtt gtacaccacc atcggcaaac
     4561 catccgctga atcaataatt gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat
     4621 agtagaacgg cgtgacggcg gagacggcat cgaagccata acgtttagcc gatgccgcaa
     4681 gttgttggct ttcagcggtg ctgacgcaac cgacgtgggc gatgagttta atcttacctt
     4741 tcgcctcttc ggcgacgatt tccagtacct gttcacgctc ggaaaggctt tgtacaaagg
     4801 cttcgccggt cgaaccaccc acgtataaac cgtcgatgcc ctgctgaata ttgaactgaa
     4861 ccaggcggcg cagactcgct ttatccagtg cttgttgttg atcaaaagga gtcaggagtg
     4921 cagccattac gccacgtaaa ttcgttgcca taaatacctc tgaagtgatg cttgtctgat
     4981 aaacgatata cctttatacc tgttatacca gatcaattaa gcaacacccc atacagaaag
     5041 cttataatgc gatctgcttc actaaagtgg cattatttct ttttgttggt ggtctgaccg
     5101 aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt gcaacgcacg atcggcttcg
     5161 tcaggatcat gacggcggat cgcatcaacg atcgcaatat gctgttgata actaacgttg
     5221 ttatgttcgt gcagtgcctg atcggtaacc gttgggcgtg cggcaataag ccagtcgagc
     5281 agggcaacgt ggatcgccat gaagattggg ttaccgggga tctccgccag cacgcggtgg
     5341 aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca gcgactgact gttgatttcc
     5401 agtgcttttg ccagcaaatc gatttgctca tcggtggcgt gttcagccgc atagcgcacc
     5461 aggctggatt caaagaacag acgtaattgt tcgaaatggg caatcccacc aggatgagaa
     5521 aggaaatctt tcgccatgcc ggaaagctcg ccgatgatgg tgtccgcaga aggtcgagag
     5581 acacgagcgc gttcgccgtt gtttatttgc accagacctt tgcgttttaa cgctgccagc
     5641 gcttcacgca cagaaggacg cccgacgtta aagaacgcca tcagttcgcg ttcagacggt
     5701 aattgttcac cttcgccaaa ttcacgacgg cggatcatct gttccagctc ttcttccacc
     5761 atttcggaga gttttttacg cgccagcggg cggctacgca agttgcgacc aattgcaggt
     5821 gaagaatctt cggtttgcga atcaaatgcg ttcataaggc ccattctgta aggtcagtgt
     5881 gattaacatc atcagtgaca tcctatcaca ggattgaaag taggggaaaa tggcaggttt
     5941 ttctctttgt gcctcatcat taccataatt aacggaataa ttaactattg cgaaaaatta
     6001 atgtaacgca gataaacaca tcccgattga attattcata agactattca caagcattat
     6061 gaatattatg aatgtgttct tacaaaataa tcataatcgc atatttttta atgaaaaatt
     6121 acctcaccta caattaaaaa cacgacatcc gcaccataaa tagacttgca aaaaatataa
     6181 catcattgtt ttcaatctgc catttatggg attgaccgtt ttcttttgac acggagttca
     6241 a
//
LOCUS       DAJTYG010000159         6001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTYG010000159 DAJTYG010000000
VERSION     DAJTYG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.160"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..1433)
                     /locus_tag="OO568_003727"
     CDS             complement(<1..1433)
                     /locus_tag="OO568_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6642876.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYL"
     gene            complement(1471..3156)
                     /locus_tag="OO568_003728"
                     /pseudo
     CDS             complement(1471..3156)
                     /locus_tag="OO568_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            3359..3481
                     /locus_tag="OO568_003729"
     CDS             3359..3481
                     /locus_tag="OO568_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642877.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            complement(3447..4602)
                     /locus_tag="OO568_003730"
                     /pseudo
     CDS             complement(3447..4602)
                     /locus_tag="OO568_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(4654..6001)
                     /locus_tag="OO568_003731"
                     /pseudo
     CDS             complement(4654..6001)
                     /locus_tag="OO568_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
BASE COUNT         1686 a         1265 c         1310 g         1740 t
ORIGIN      
        1 agatagttgt catagtcata acgcacaccg ggcattaacg acacattccg ccagctaatg
       61 tgatccgcca tatacagcgt gtagttgtca attcccaggc tgcctttacc tttatggtaa
      121 atggtatggt tagttttctt tcctgcagca ttaatcacat aggattcaga ctggttatgg
      181 cgttcagtcc atgcatcgga atagatgtat tccgcgccga agtagggttg atgcgaaaca
      241 tcacccacgg cgaatttttg ccagtccagg cgtgttttga aggtgtaatt atctacagcc
      301 tgggaaatgt gtcctaatcc gccacgggta caacgccctg taatatcacc atatgtacat
      361 gaaagttcgg tgtaccagat gtcatgatcg tgacgggtat aatcactaat atgatcccaa
      421 ccaacggtgg tacgtagttt ggcccatgcg agttgcgtat ccatatccca tgccagacca
      481 taggatttat tacccatttc gcgatcagat tgcgggaagg tgctggtatt ataatcacgg
      541 ctggagccgg tatattttaa ggttaaatcg tgggtaaagc ggtcgctggc aaaccaggta
      601 aatttgctca atgcagtatc gataacgttt ttatactgtg cccgaccggc gacaatgccg
      661 tcattcgaaa cataatccgc gcgggtgata tcagactggc ggcgcgataa accggcggta
      721 acgccaaagt tatcagccag ctcctgatta aacgacaagg tataaaagtt ctttttaaaa
      781 tctggagaga aataggtact tcctgaagaa ccttgattaa atgcgctctt gttattctca
      841 tcgatatgcg atgttaacca gtccaaacgc gtagtgcgat aacccaattt caccttgcta
      901 tcatcagcgt tgaagcgttt gatctttgca tcaattaccc cgccattgaa gcgaccaaat
      961 tcaaccggca caaaactgtc ataaagcgtc acattgtcca gtaagctgac atcaagataa
     1021 tacccctgtg acatcccgct aatattggtt gcactactgg catcggactc attcgctggg
     1081 ttcaggttat tagttgcact aataccgtca atcaaatagg cattctggta gggcgacgca
     1141 ccgtgaatag agattttctc agggcgaata tctccctggt tcaacgaggt actttgcgtt
     1201 gaatccatgc gtaccgcagg gttggttctc agcagatcgc tgatattgcc attcccggtc
     1261 ggtaaacgct cgatgctttc actggtgtaa tgcgtgttgc cgtttacggg tacagggaca
     1321 ggagcataga ccgtcatctc ttcaaaaaaa tgcatgtaca tgtttttgtc atcgacggcc
     1381 tgcgccacat cagcgccaca taaaatgacg ccaggaataa gaactcgctt catttattta
     1441 tatctccgta cagcagggct tttattgttg ttataaaacc gtgctaatgt cacaaatatc
     1501 atcagccagg ttccagacac cgggttgatg tgtaaccata atgacgccgc ttgtgggtag
     1561 tttttcacgc actaaacgca gtaagcgtat agcctcttgt tcctcaagat gagaggtagt
     1621 ttcgtcaaga aatatccatt tcggacgtcg taaaattaat cgtgccaggg cgatacgttg
     1681 tttttcgccg ctggaaagaa tatctcccca gcgatcgtgg tcatgaatac gcgcagccaa
     1741 tttccccaga ccaacctgat gcagtacttc gctcaacgat ttatcgtcta cgggcagggg
     1801 aagtgcttta caaataatct ctttcagtaa gccgcttttg attaacggtg tttgtgacac
     1861 ataccaactg tcagcaggag aagaaatatc acctttaaat cacggccagc agtgggataa
     1921 tgttttaagc agtgtggttt ttcccgcacc agagtagcct ttcagtaata gccatttgcc
     1981 tggcgaaaca tgaaagttca agttctctaa tatgatctta ttatcaggcg tacgaatact
     2041 cgcattagcc acttgtaccg catgttggca atttttaggc ttattcgtag ggcgctattc
     2101 agtgagttga tggaactcat acaagcgatc gataaccgca gccagttcag caagttcgtc
     2161 atatttataa ataaaccagc ttaaattgtt cgatactagc ataaatgcct ggcgcgattt
     2221 catcagtccg cccagattga tctgcccgct aataaactgc ggcaataaca gaaagtaggg
     2281 aagaacgcta agcgagcgcg aataaatatt ttgccagtaa tcaagccacc gctgacgatt
     2341 cattaaacga tgccagttct ctttaatggt atgaaaattg tcgcgcaact cctggcgttg
     2401 tagagactca gcattgctta gcgcaatcag ttctgcctgc ttattatgct gcacaagatt
     2461 agttcgaaat gtcgcttcgc tacgttgttt ttccacatta agcggacgaa tacgtttacc
     2521 caccttatgg gtaaataaag ttccaccgat cacaatgagc acgacggtat agaccatata
     2581 cccctggata ttccattctg ttccaccaac agtgaatgag agcgtacccg cgctttgcca
     2641 aagaataacg gtaaaggtga tcagcatgct aagtgactgg atgaagccaa atgaaagact
     2701 gagcgttttg ttgatcagta agagaatgtc ttcagcgata cgttggtcag ggttatctgt
     2761 atttttatgc tcgccgtaga tctgcgtgaa gtagtagttt ttatctgcaa accagcgatt
     2821 aaggtagtaa tctgttaacc attcgcgcca gcgaatagtc agtagtttaa ttaaccatgt
     2881 tttgtttacg gatattaata caaagatccc cagcagggca gggaaccaaa gaacaagctg
     2941 ccaaagctta tcggtttctt tctggcttag cgcattgaaa aaatcattat tccagtcgtt
     3001 taaccagacc tgaattttaa ccacgccaag gatcattgcg aggataataa tgattaacaa
     3061 cactgaagtt ttattattct tacgcagcca aaagggcttc agcagacaga gatactttgc
     3121 tatcaacata caaaacgtaa tgggaatggt tatcattagc gaaaattgat tgtgccatcc
     3181 aatgattatc tatggcaatg atttactgat gaaatttatt taagtatcat ttaacaatat
     3241 gaaatatata gaaattttta attttcagtg gttatggtta tgctattgat aatcaagggt
     3301 attgctaatg ccctgacgca taccacgtaa gccaggattt tcgcaaggga agatgatgat
     3361 gacgaaacac ccgacaggaa tttatgtcgg gtgccttgtt aaggtcataa gaaggaggct
     3421 aagaatggag ttaaaagaga gcgttattaa ttattctcca tttgttttgc aacatccata
     3481 atgtggtaaa gcggtactct gttcttagct aactcaacca tggcgttcat ataaggtacc
     3541 atggttgaaa aaaggatttt ataaccttcg caaaaataag aaacagtctc attgtttact
     3601 ttagtaatac gatgcttagg acaaccgcca ttgcagatag gtttatatgc acattgctga
     3661 catttcgctg aaatccgttt tttgcgctgt cagttgtaca ctgttcatcg ttttgagttc
     3721 agatttatta atgtttccaa ttttgtactg tggatagaca aaatggtcgc attcgtaaat
     3781 gtctccatta ctttcaacaa ccagattatc cttgcaggac tcctggaaaa tacaacttgt
     3841 atgcccattc cccaaaaaac ggctgacaaa gctttcaaac tgacgaatga aaatttcacc
     3901 cacatcgttt ttaacccatt gcataaaaat ggttgacata aacttgccat aagccgtggg
     3961 aggcacagaa aaatcaatga tacggaatgt gttctcacta tgaccactga aatcaatatt
     4021 cggcgtcccg gtttctagca attcgataaa ttgcatatgt ttactgccga tagattttaa
     4081 aaaatgataa acctcaagag ggtaatggac attaacgtta ttaatgacgg ttaacgtatt
     4141 aaactctact tgatatgatt tcagacgctc gatggctgct atcacttttg caaaagtacc
     4201 gttacctgaa ttactgcgtc tgtaacggtc atgtaactcc tgggggccat cgatcgagat
     4261 accaaccaga aaatcatgtt ctttgagaaa ggcacaccat tcattattca ataaaatgcc
     4321 attcgtttgt aatgcattaa aaatacgttt ttggcctgca tagcgttgtt gatagtgaat
     4381 aactttacgg aaaaaatcca ggccagccag agtgggttca ccgccttgcc aggtaaaata
     4441 gacctgattg ccagatgctt cgatatattg tttgatgaac tctttcagag tgctgtcatc
     4501 catccatttt tcatgagtaa actgcgactc tttttcaagg taaaaacagt aatcacattt
     4561 gagattacat tgaaaactgg agggcttggc tgtaacgtgc attgctatct cgctcaataa
     4621 ggcggcggaa aaatccgccg catgaaggtt tagttatttc gcttcgctta gtgctttctt
     4681 gatattgtta aacttctcct gatttacctc gctaagcggt ggctggctgc tgtcgataaa
     4741 ctctcttacc acgccttgca tctctttaac gacctgcgga ttggcggcgg caaggttatc
     4801 tttttgctgt agatccgtca gtttgtagag acctaactga ttgttttcta ctgtatagac
     4861 aagcgaataa tcgttatttc tcaccgtata agagaattgg cttaagtcct cagtgttggg
     4921 gttatgcggg taatcgtctg actgatggcg aacaaatttg tggtaattat cccagaatgg
     4981 aatattttcc tcatcaaacc agtgagaata agaggttatc caggtcagat ttttatgtgg
     5041 ctcgccttgt ttcttatctt gcaaccaggg cagcaaggaa acaccatcca gcttaaggtc
     5101 ttttggaatg ctgatatcgg ctgcatcaag agctgttggg tagaaatcca ttgcggaaat
     5161 cagcttgtca taattaccgg gttgaagttt tcctttccac cacataaaca ttggggtggt
     5221 gagtaccgcc aggataggtc tgactcttat agcctttttg cgccccgttc agcggcagag
     5281 gaccatcgat aaccgcacca ttatcggagg taaagagaat aattgtattg tcatactgtc
     5341 cgtttttctt cagttgttcg agaatgcgtt ttacaccctg atcaacagaa taaacggaag
     5401 cgtagtagtt atctgctgtt tgactaccgg tattaaattg cttctgatat tgatccggtg
     5461 caggattatc atttggtagg tgcggagcat tataagccag gtaaagcata aaaggctggt
     5521 caagtgtttt ggcacgatca acaacgccaa ttgcctcatc ggttaactga tcgctgatat
     5581 aaccttttgc ggggacacgt tcacgatttt tgaacagtga aggggagttg taatatgccg
     5641 ttcctgcagc gtggaatccc ataaagtaat caaagccacg gttttgaggt tgccattctt
     5701 ccgcagaaaa tgtggtgaag ttgtcatgat agtcacgcgt ttgtttatct tccggtaccg
     5761 gcacattact gatttttgac aagtgccatt tacctactgc tgcagtgtaa taaccatgat
     5821 tctggaataa ttcaggcaag aaagtttctg ttagcggaat accatcctga gcatcggtat
     5881 tggaatagac accaacgcgg gcgggagctc gaccggtcat tattgcggca cgggaggggc
     5941 cggaaacacc gtgtgccaca tagccgttag taaaacgtac gccttcatcc attaatgaaa
     6001 g
//
LOCUS       DAJTYG010000160         5988 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTYG010000160 DAJTYG010000000
VERSION     DAJTYG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.161"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..258)
                     /locus_tag="OO568_003732"
     CDS             complement(<1..258)
                     /locus_tag="OO568_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6642878.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEK"
     gene            795..2696
                     /gene="mtlA"
                     /locus_tag="OO568_003733"
     CDS             795..2696
                     /gene="mtlA"
                     /locus_tag="OO568_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6642879.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGNVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            2791..3939
                     /gene="mtlD"
                     /locus_tag="OO568_003734"
     CDS             2791..3939
                     /gene="mtlD"
                     /locus_tag="OO568_003734"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6642880.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            3939..4526
                     /gene="mtlR"
                     /locus_tag="OO568_003735"
     CDS             3939..4526
                     /gene="mtlR"
                     /locus_tag="OO568_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6642881.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(4538..4747)
                     /locus_tag="OO568_003736"
     CDS             complement(4538..4747)
                     /locus_tag="OO568_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642882.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5031..5393
                     /locus_tag="OO568_003737"
     CDS             5031..5393
                     /locus_tag="OO568_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6642883.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1563 a         1453 c         1487 g         1485 t
ORIGIN      
        1 tttctcctgc gacgcaacat gtgactgcat accccagcca cgctccggtt gatagagcgt
       61 cgcccagatc cacagaaacg gccagataac atgcagagta aacaggctca cccagcccgc
      121 cgtatgaata gcgtcggcat gggggtgatt gcgctttttg gcaatcagat aagggatgtc
      181 atgtatcgcg atgattccat aaaaaatcac cagaaagacg aagatcagca ctcccaacgc
      241 gaaatagttt agaaacatat ctgcctcgga ttcacgttta tcagtgttgt tttttggctg
      301 gcagccagaa gggagtcagg ctgatatttt gacaataatc cgggttcgcg attctcgcca
      361 taacaccaaa gaataatttt tagaggtgat gagttgctta gttacataac gattgtatga
      421 cgaaggcata acatgctgta gatcacatca ggtgaacgcc gtaagaaaat atcttgtgat
      481 tcagatcaca aagcttcaac aagccatcaa aacaaaaatg tgacattact cacatttaaa
      541 tgccattttt agcgaaaatc accgccttgt ttctttttta cacaggcgtt ttgtgatgag
      601 cgtcacgtca attacctctc taccccctat atttatgtga ctgatatcac acaaaagccc
      661 gtcgactgga cagttaaccg attcagtgcc agatttcgca gtatctacaa ggtccggcta
      721 cctctgccgc cacattaaca aaaaacctcg ggcttccagc ctgcgcgaca gcaaacataa
      781 gaaggggtgt ttttatgtca tccgatatta agatcaaagt gcaaagcttt ggtcgtttcc
      841 tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc gtggggtatc atcaccgcgt
      901 tatttattcc aacagggtgg ttaccgaacg agacgctggc gaagctggtc gggccgatga
      961 tcacttatct cctaccgctg ctgatcggtt ataccggtgg taagctggta ggcggcaaac
     1021 gtggcggcgt agtcggtgcc attaccacca tgggcgttat cgtcggcgca gacatgccga
     1081 tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg gtgcattaag cacttcgacc
     1141 gctgggtaga cggtaagatc aaatccggtt ttgagatgct ggtgaataac ttctccgcag
     1201 gcatcatcgg gatgatcctc gctattctgg cattcctcgg cattggcccg attgttgaag
     1261 ccctgtccaa aatgctggct gcgggcgtta acttcatggt tgtccatgac atgctgccgc
     1321 tggcgtctat ctttgttgaa ccggcgaaaa tcctgttcct caacaacgcc attaaccacg
     1381 gtatcttctc gccgctgggt attcagcagt cccatgaact gggtaaatca atcttcttcc
     1441 tgattgaagc taacccaggt ccaggtatgg gcgtgctgct ggcgtacatg ttctttggtc
     1501 gtggtagcgc taaacagtct gcgggcggtg cggcaatcat ccacttcctg ggtggtatcc
     1561 acgaaatcta cttcccgtat gtgctgatga atccgcgtct gatcctcgca gtcatcctcg
     1621 gcggtatgac tggcgtgttc acgctgacta tcctgggcgg tggtctggtt tctccggcat
     1681 ctccgggttc tatccttgct gtactggcga tgacaccaaa aggtgcttat ttcgctaaca
     1741 tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt ctctgctatt ttgctgaaaa
     1801 ccagcaaagt gaaagaagaa gatgatattg aagcagcaac tcgtcgtatg caggacatga
     1861 aagctgagtc taaaggcaca tctccgctat ctgcgggcaa tgtgactaac gacctgagcc
     1921 acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg ttcaagtgcg atgggcgcag
     1981 gcgtgctgcg taagaaaatt caggatgcag gtctgtcgca gatttcggtc actaacagcg
     2041 cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac tcaccgtgac ctgaccgaac
     2101 gcgctatgcg ccaggttccg caggcacagc atatttcgct gaccaacttc ctcgacagcg
     2161 gcctgtacac cagcctaacc gaacgtctcg ttgctgccca gcgccatact gaaaacgaag
     2221 tgaaagtgaa agacagcttt gacgattcca gtgctaacct gttcaaactg ggcgcggaga
     2281 acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt gctggcgagc
     2341 agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg gatcgtgaaa
     2401 aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg gttgaagcga
     2461 aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc gtgcgcttcg
     2521 gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgcagcccgt aacaacgagc
     2581 acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc atcgagcgtc
     2641 tggcacacac caccagcgtg gatgaagtgc tggaactgct ggcaggtcgt aagtaatcca
     2701 atcctaccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc ccagccctct
     2761 cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat
     2821 atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact gacgtttgcc
     2881 gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca ggttcatgtg
     2941 gtcggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag cagcattggt
     3001 gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt tggcccggtt
     3061 gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt
     3121 aatgaatccc cgctgaacat catcgcctgt gaaaacatgg tacgcggcac cacgcagctg
     3181 aaaggtcatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga agagcacgtt
     3241 ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc aactaacgat
     3301 ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac gcagttcaaa
     3361 ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc atttgtcgaa
     3421 cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg aaaactggcc
     3481 ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt
     3541 gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc agacaaacat
     3601 gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa agatgatgta
     3661 gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca
     3721 ctgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg gattgctggt
     3781 gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc actgatcgct
     3841 gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa cagcgaggtt
     3901 gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggaca
     3961 ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggcct
     4021 ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca
     4081 ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag
     4141 acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt ccgctgggcg
     4201 atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg
     4261 aagatgcgga actgctgatg gcattgcgtg aagagctaaa tcacgacggc aacgagtacg
     4321 cctttaccga cgacgaaatc cttggaccct ttggtgaact gcactgcgtg gcggcgttac
     4381 caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa attcagcgct
     4441 atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt tccaaaatca
     4501 gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct acctgcttcg
     4561 gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg ctttcatcgg
     4621 gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc cggaacgcct
     4681 ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt
     4741 tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt
     4801 ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat aatcatccgc
     4861 tgctgtttat tatctttttc tatagatttc ttaattaatc aacgaattgc gtagtttttg
     4921 agcatgttaa tgattacgtt gcagaactat tacaatgtca tcagttgtga acaaagcacc
     4981 tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag
     5041 tcgaaaaaaa cgaaatcaaa cgtctcagcg atcgcctgga cgccatccgc caccagcagg
     5101 ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca
     5161 cgctggaagc agaaattgct cgcctgcgtg aggttcatag ccaaaaactg agtaaagaag
     5221 cacagaagct gatgaagatg ccgttccagc gcgcaattac caaaaaagag caggctgata
     5281 tgggcaagct gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg
     5341 ggcgcgaaat gggcctggaa gagatgactg gattttctaa gactacgttt taagcccctc
     5401 agacgactgg ttcgatcgta tcaacccgca tctggcttgc cttagctggt caatgcgggg
     5461 actctcctgt ctactcgcct aattaccaaa caattcactc cctttacttc gatttaccgc
     5521 tggttgatat tctgaaaatg catttttacc gaaatagcca tctataaaca ataaaaatat
     5581 atagaagata tcatttgtta attaacaagc atttacgtct ctttttttcg gatttaattg
     5641 tgaaatttta atcatcatat tcacaaaatc aattatctgt aaaatttttc ccagacatgg
     5701 tttctttcat atttgacatt tattaataat catcattaga atccacgaaa aataatatat
     5761 tcatacaaac acagacatct tttgaaacga atgatacatt tttatgatga atgcttaaaa
     5821 caatatttaa tcgacacaca tcactgtcat taatgtaaag caacatagct caccaataat
     5881 acaatggaca actctaccgg cattgatcgc tggtcggagg aaataaggat ttttacatga
     5941 acttgaagaa gatttttttc agtgctgtca ctgtttcagt tctctgcg
//
LOCUS       DAJTYG010000161         5981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTYG010000161 DAJTYG010000000
VERSION     DAJTYG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.162"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            40..1113
                     /locus_tag="OO568_003738"
     CDS             40..1113
                     /locus_tag="OO568_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6642884.1"
                     /translation="MNKYIKQWCFSVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            1361..2401
                     /gene="pstS"
                     /locus_tag="OO568_003739"
     CDS             1361..2401
                     /gene="pstS"
                     /locus_tag="OO568_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6642885.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            2488..3447
                     /gene="pstC"
                     /locus_tag="OO568_003740"
     CDS             2488..3447
                     /gene="pstC"
                     /locus_tag="OO568_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6642886.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            3447..4337
                     /gene="pstA"
                     /locus_tag="OO568_003741"
     CDS             3447..4337
                     /gene="pstA"
                     /locus_tag="OO568_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6642887.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            4429..5202
                     /gene="pstB"
                     /locus_tag="OO568_003742"
     CDS             4429..5202
                     /gene="pstB"
                     /locus_tag="OO568_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6642888.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            5217..5942
                     /gene="phoU"
                     /locus_tag="OO568_003743"
     CDS             5217..5942
                     /gene="phoU"
                     /locus_tag="OO568_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6642889.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGSDELDKLLAGKDSDK"
BASE COUNT         1486 a         1426 c         1554 g         1515 t
ORIGIN      
        1 taattgaaca gggtgtgaca tgtcattaaa aggattttta tgaacaagta tatcaaacag
       61 tggtgctttt ctgtgtttat gctctcgtta agtagtgtag cacttgcagc tcctaaaggt
      121 atctgtaccc ctgataatgg cgtatttcac agtacgcttg atttctcggg atatcttatt
      181 accgcgaacg aaaataaggt tggaacgaca tttaacacca ctgtaactaa tggatcctca
      241 tatcttggac gctgtcattg tgatactggt aacgtaggag aattccccta tatctattat
      301 acgtcaaaaa taaaccaggc attaacttat gctggggttc actctaatat taattattat
      361 gatcttaatc cgaaccttga tgttgggatt gctatagata ttcttggggt tgggtatgtg
      421 aatgcacctt ttgaatatca tgctaacaac ccgtctggta atacaaagta caattgcaac
      481 cgcattgaac ctttaagtat atccagtggg gctaaagcga tagtatattt ttatatcaag
      541 aagacatttg caggaaaatt gattattcct gaaacaaaga tagtgacatt gtatggaaca
      601 attagccgtg acaccccggt ggattactca cagccgatgg ctgatgttta tattcggggc
      661 gatattacag ctccgcaaag ttgcgaaata aataatttaa agccagtttg ttttgatttt
      721 aaagaaatac cagctgcaga tttttcatct gttgttggaa gtgcggtaac aacacataaa
      781 attactaaaa cagtcactat tgagtgtgaa aacctaggga tactgaatac cgatgatatc
      841 agtacgtctt tttatgctac cgaacccaat acggacaatt caatggtcgt aacttcaaac
      901 tctaacgtgg ggataaaaat ttacgacaaa aataataagg aaatcaaagt gaacggtggt
      961 gagttgccaa cagacatgga taaatcaaca gtgtatggtg aaaaatcagg tagtgtaact
     1021 ttttcagctg ctcctgcgag ccttactgga gctcgcccag ccccggggca atttactgca
     1081 acagcgacga taactgttga gattgtgcgc taataggtct gacaacgatt gttcatcaca
     1141 tcgttgtcat ctcacccatt tctttatcac ttttaaatgc atcatgacaa aaagatggca
     1201 tgtgggattt tcatcttcac atttaaaatc agtaagttat ttggtaattc ctttctgtca
     1261 taaaaatgtc atattcctta tatataactg tcacctgttt gtcctatttt gcttctcgta
     1321 gccaacaaac aatgctttat gaatcctccc aggagacatt atgaaagtta tgcgtaccac
     1381 cgtcgcaact gttgtcgccg cgaccttatc gatgagtgct ttctctgtgt ttgcagaagc
     1441 aagcctgaca ggtgcaggtg caaccttccc tgcgccggtg tatgccaaat gggctgacac
     1501 ttaccagaaa gaaaccggta ataaagttaa ctaccagggt atcggttctt ccggtggcgt
     1561 aaaacagatt atcgctaata ccgttgattt tggtgcctct gacgcgccgc tgtctgacga
     1621 aaaactggct caggaaggtc tgttccagtt cccgaccgtg attggcggcg tggtgctggc
     1681 ggttaacatt ccagggctga agtctggcga actggtgctg gatggtaaaa ccctcggcga
     1741 catctacctg ggcaaaatca agaagtggga tgatgaagcc atcgccaaac tgaatccggg
     1801 tctgaaactg ccttcacaaa acattgctgt agtacgccgc gcagatggct ccgggacttc
     1861 cttcgtcttc accagctacc tggcgaaagt gaacgaagag tggaaaaaca acgttggtac
     1921 tggctctacc gtaaaatggc cgatcggtct gggcggtaaa ggtaacgacg gtatcgccgc
     1981 gttcgttcag cgtctgccgg gtgcaattgg ttatgttgaa tatgcttacg cgaagcagaa
     2041 caacctggcg tacaccaaac tgatctccgc tgatggtaaa ccggttagtc cgaccgaaga
     2101 aaacttcgct aatgcagcaa aaggtgcaga ctggagcaaa accttcgctc aggatctgac
     2161 caaccagaaa ggcgaagatg catggcctat tacctctacc acgttcattc tgatccacaa
     2221 agatcagaag aaaccagaac aaggcacaga ggtgctgaaa ttcttcgact gggcgtacaa
     2281 aaccggggct aaacaggcga acgacctgga ttacgctagc ctgccggata gtgtagttga
     2341 acaggttcgc gctgcgtgga agaccaatat taaagacagt agcggtaagc cgctgtacta
     2401 ataaaactcc aggccgggta cggtgtttta cgccgcatcc ggcattacaa aatgactttg
     2461 taaacgcgtt taactgaaga gtaacttatg gctgcaacca agcccgcttt taacccaccg
     2521 ggtaaaaagg gcgacataat tttcagcgtg ctggtaaaac tggcggcgct gattgtgcta
     2581 ttgatgttgg gtggcattat tgtctctctg atcatctcct cctggccgag cattcagaaa
     2641 tttggtctgg ctttcctatg gaccaaagag tgggatgcac cgaacgatat ctacggggcg
     2701 ctggtgccga tctacggtac gttggtgact tcgtttatcg cgctgctgat cgccgtcccg
     2761 gtgagtttcg gtatcgccct gttcctgact gagcttgcgc ctggctggct gaaacgcccg
     2821 ctgggtatcg ccattgagct gctggcagcc attccaagta tcgtttacgg catgtggggc
     2881 ctgtttatct ttgcgccgct gttcgccgtt tactttcagg agccggtcgg caatatcatg
     2941 tcgaatatcc cgattgttgg cgcgctgttc tctggccccg catttggtat cggtatcctc
     3001 gcggcaggcg tgatcctcgc catcatgatt attccgtaca ttgcggcggt aatgcgtgat
     3061 gtgttcgaac aaaccccggt gatgatgaaa gagtcggcct acggtattgg ctgcaccacc
     3121 tgggaagtta tctggcgtat cgttcttccg ttcaccaaaa atggtgttat cggcggcatc
     3181 atgctggggc tgggccgcgc gctcggtgaa accatggcgg tgacctttat catcggtaac
     3241 acctaccagc tcgacagcgc ctcgctgtac atgccgggca atagtatcac ctctgcgctg
     3301 gcgaacgaat ttgcggaagc ggaatccggt ctgcacgttg ccgcactgat ggaactgggc
     3361 ctgatcctgt ttgtgattac cttcatcgtc ctcgccgcat cgaagtttat gattatgcgc
     3421 ctggctaaga atgagggggc acgctaatgg ctatggttga aatgcaaacc actgcggcgc
     3481 tggctgaatc tcgccgcaaa atgcaggcgc gtcgccgcct caaaaaccgt attgcgctga
     3541 cgctctcgat ggcgacgatg gccttcggcc tgttctggct gatctggatt ttaatgtcca
     3601 ccatcactcg cggtatcgac ggtatgtcgc tggcgctgtt cactgaaatg acgccgccgc
     3661 ccaatacgga aggtggtggt ctggcgaacg ccctggcggg tagcgggctg ttaattttgt
     3721 gggccacggt attcggtacg ccgctgggca ttatggcggg gatttatctg gcggaatatg
     3781 gtcgtaaatc ctggctggca gaagtgattc gcttcattaa cgacattctg ctctccgcgc
     3841 cgtcgattgt ggttggtctg tttgtctaca ccatcgtggt ggcacagatg gagcacttct
     3901 ccggctgggc gggcgtaatt gcgctggcgc tgttgcaggt gccgatcgtt atccgcacca
     3961 ccgagaacat gctgaaactg gtgccggaca gcctgcgtga agcggcttat gcgctgggta
     4021 caccgaagtg gaagatgatc tccgcgatta cgctgaaagc gtcggtgtcc gggattatga
     4081 ccggtatcct gctggcgatt gcccgtattg ccggtgaaac cgcgccgctg ctgtttaccg
     4141 cgctctccaa ccagttctgg agcacggaca tgatgcagcc gatcgccaac ctgccggtga
     4201 cgatctttaa gtttgcgatg agcccgtttg cggaatggca acaactggcc tgggccgggg
     4261 tattgatcat taccctgtgc gtcctgctgc tgaacattct ggcgcgcgtt gtttttgcga
     4321 agaataaaca cggttaacac tttgccggat gcggcgtgaa cgccttaccc ggccctacgg
     4381 taagcctgat tagcgaaaag catcaggtac gatgaggaaa agattgcaat gagtatggtt
     4441 gaaactgccc cgagtaaaat tcaggttcgt aatttgaact tctactacgg caaattccat
     4501 gccctgaaaa acatcaacct ggatatcgcc aaaaaccagg ttacggcgtt tatcgggccg
     4561 tccggctgcg gtaaatcgac gctgctgcgt accttcaaca aaatgtttga actgtacccg
     4621 gagcagcgtg cggaaggtga aattctgctt gatggcgaca acatcctgac caactctcag
     4681 gatatcgccc tgctgcgtgc gaaagtgggc atggtgttcc agaaaccgac gccgttcccg
     4741 atgtccatct acgacaacat cgcctttggc gttcgtctgt ttgagaagct ctcccgtgcc
     4801 gacatggacg agcgcgtgca gtgggcattg accaaagccg cattgtggaa cgaaaccaaa
     4861 gataaattgc accagagcgg ttactctctc tctggtggtc agcaacagcg tctgtgtatt
     4921 gcgcgtggta tcgccattcg cccggaagtg ctgctgctcg acgaaccgtg ttcggcgctc
     4981 gaccccatct ctaccgggcg tattgaagag ctgatcaccg aactgaagca ggattacacc
     5041 gtggtgatcg tcacccacaa catgcagcag gctgcgcgtt gttccgacca cacggcattt
     5101 atgtacctgg gcgaattgat tgagttcagc aacacggacg atctgttcac caagccagcg
     5161 aagaaacaaa cagaagacta catcaccggt cgttacggtt gattcaggag tgcgttatgg
     5221 acagtctcaa tcttaataaa catatttccg gccagttcaa cgccgaactg gaaagtatcc
     5281 gcacgcaggt gatgaccatg ggcggcatgg tggagcagca gctttctgat gcaatcaccg
     5341 cgatgcataa ccaggacagc gatctggcga agcgcgtgat cgaaggcgac aagaacgtca
     5401 acatgatgga agtggcgatc gatgaagcct gcgtgcgcat tatcgccaaa cgtcagccga
     5461 cggcgagcga cctgcgactg gttatggtga tcagtaaaac cattgccgag ctggagcgta
     5521 ttggcgacgt ggcggacaaa atctgccgta ctgcgctgga gaaattctcc cagcagcatc
     5581 agccgttgct ggtaagtctg gagtcgctgg gccgtcatac catccagatg ctgcacgacg
     5641 tgctggacgc gttcgcgcgg atggacattg acgaagcggt acgtatttat cgtgaagata
     5701 aaaaagtcga tcaggaatac gaaggtattg ttcgtcaact gatgacctac atgatggaag
     5761 attcgcgcac tattccgagc gtacttaccg cgctgttctg tgcgcgttca atcgaacgta
     5821 tcggcgaccg ctgccagaat atttgtgagt ttatcttcta ctacgtgaag ggtcaggatt
     5881 tccgtcatgt aggcagtgat gagctggata aactgctggc gggaaaagat agcgacaaat
     5941 aattcaccag acaaatccca ataacttaat tattgggatt t
//
LOCUS       DAJTYG010000162         5980 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTYG010000162 DAJTYG010000000
VERSION     DAJTYG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.163"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            241..513
                     /locus_tag="OO568_003744"
     CDS             241..513
                     /locus_tag="OO568_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6642890.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(479..886)
                     /gene="nudG"
                     /locus_tag="OO568_003745"
     CDS             complement(479..886)
                     /gene="nudG"
                     /locus_tag="OO568_003745"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6642891.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            973..1602
                     /locus_tag="OO568_003746"
     CDS             973..1602
                     /locus_tag="OO568_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6642892.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKSGITPDGLTLVGFAIGVLALP
                     FLALGWYLAAPVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1611..2918)
                     /gene="ynjE"
                     /locus_tag="OO568_003747"
     CDS             complement(1611..2918)
                     /gene="ynjE"
                     /locus_tag="OO568_003747"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR6642893.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDTLSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPVGGWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPGSSK"
     gene            complement(2985..3638)
                     /locus_tag="OO568_003748"
     CDS             complement(2985..3638)
                     /locus_tag="OO568_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6642894.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3638..5172)
                     /locus_tag="OO568_003749"
                     /pseudo
     CDS             complement(3638..5172)
                     /locus_tag="OO568_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(5145..>5980)
                     /locus_tag="OO568_003750"
     CDS             complement(5145..>5980)
                     /locus_tag="OO568_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6642895.1"
                     /translation="LLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTF
                     IARRDVTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKE
                     APDDATFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAH
                     AQQKIASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRK
                     ADPVVWGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGT
                     H"
BASE COUNT         1413 a         1610 c         1583 g         1374 t
ORIGIN      
        1 tccatagata taaaaacctt atatattaat acgattgtgc ttatgttgtt tatgtcatgt
       61 gcttttgcag ttttcgacta taccatcaag aatgtgacgg taagcaagaa aaaaatgacc
      121 caggaaagca aattaataac gagagtaatc tcataaccaa atgcgataac tcagacgcta
      181 aagttttgcc acccggctac cgttatcagt agcagagaca ataacgttaa ggagttccct
      241 gtgagtcgag cattgttcgc cgtagtatta gcatttccat taattgcact ggctaacccg
      301 cattatcgtc cggacgtgga ggtaaacgta ccgccagaag tgttcagttc aggcgggcag
      361 tcagcgcagc cctgtactca gtgctgcgtt tatcaggatc aaaattattc tgaaggggcg
      421 gtaattaaag ccgaaggaat actgctgcaa tgccagcgtg atgacaaaac ccttagcact
      481 aatccgctgc tctggcggcg cgtaaagcca taaacgcctc taataatgga atgtcagcag
      541 gggccagcgg atattgcagc gcctcttcag gtgagcacca gaccagcgcc tgatgttcat
      601 gtgcctgtaa cgtcccgtgg aagtcgggta cgtgccaggc atgaagatgg ataatccggc
      661 ccgaaacttc tcgctgatgg ctggcaacat actcacccac agttgcttcg atgtccaatt
      721 cttcgtttaa ctcacgcacc agcgcctgct gctggctttc atccagctcg actttacctc
      781 cggcaaactc ccataatccc gcctgatcgc tctgggcggg gcgttgcgcg agtaaaattt
      841 tgccatcacg ttcaatgatg gcggcaacaa cttcaatcat tttcataaat tacacacggg
      901 cagataagcg aaaaatgcca taatatccgc actgttgctc aatcagaaag gctttatatt
      961 taaggagtgc ctgtgctaga ccgccatctt catccccgga ttaaaccgtt gttgcatcag
     1021 tgcgtgcggg ttcttgataa atcgggcatt acgccagatg gtttaacatt agtgggattt
     1081 gccatcggcg tgctggctct gccgtttctg gcgctgggct ggtatctggc ggcgccagtc
     1141 gtcattttgt tgaacaggct gcttgatggt ctggacggtg cgctggcgcg gcgcagaggg
     1201 cttaccgacg cgggcggctt tcttgatatt tctctcgatt ttctctttta cgcgctggtg
     1261 ccgtttggct ttattcttgc tgcaccggag caaaatgcgc tggcaggcgg ctggttgttg
     1321 tttgcgttta tcggcacggg cagcagtttt ctcgcctttg ctgcactggc agcaaaacat
     1381 cagattgata accccggtta tgcacataag tcgttttact atctgggggg attaaccgaa
     1441 ggcacagaaa cgatcttact gtttgtgctg ggatgtttat tccctgcatg gtttgcgtgg
     1501 tttgcgtggt ttgcgtggat cttcggtgcg ttgtgctgga tgacgacctt tacgcgggtg
     1561 tggagtgggt atctgacgct gaagtcgctc cagcgtcagt aattgcaatg ttatttgcta
     1621 ctgcccgggc cgcgttcacc ggttgcaacc ggattttttg gatcgctgct ccattcgtac
     1681 cagccgccgt catacacgga aacattcttc caacccatgg cgcgtgcgta cataaaggtt
     1741 tcggacgcgc gccagccggt gccgcagtag aatgaaactt gctgatctgg tttgatattc
     1801 catgctttcc acatagcggt aatatcatcg gcgctacgca tggtgccatc cgggttatgg
     1861 aaatcttcca tatgcgtcga gtcgctacca gcgtgtcccc aacgtgctcc ggctatttca
     1921 ccttttggtt taatatagct gtaaccgctg gtcgtaccga taaattctgg ccacgaacga
     1981 atgctcacca gcgatgcatc ctggcgatgc agcagtccac gggcctgttc catatcgagc
     2041 atcaactgcg gttgtgccgg gatcttcacg ccgaaatctg gttccgcttt cactttcggt
     2101 ggcgttcctc gctcaacagg cagtcccgcg tcggaccagg tttgccagcc gccatccagc
     2161 agacgcacat ctttcacgcc tgcgtagagc ataatttgcg ccacacgcgc tccagcgtat
     2221 acgtcacgcc cgtacagaat gaccgtggtg tcatggcgaa tgccgtgttt tgccagcatc
     2281 gcttttagtt gttcatcaga aactttgttc cacagcggtt cgctttccac ttcgttggta
     2341 tcgatgtagt cagcgccagg aatatggctg ataaggtaaa gcttaggagc gccccaggcc
     2401 gcttcaatga ctttccagcc accgacaggt ttcgccgtaa cctctttacc ttgttgcagg
     2461 tcatgcagcc attgcggata aaccagctgc tcaaagtgcg gcagtttttg tagacgggaa
     2521 ggttcgctta gcgtgtcact cagaatggag atatgcgtaa aacctgcttt ttgcagtcgc
     2581 gttttgacgg cgtcgacatc tttgccatta ccgtacagcg ccaccggggc atcggctttc
     2641 aggttatgtt gcttgatcca cgcgttgagc tgttcggtgc tcattttgtc gagccagcta
     2701 gcagagaggt ttaaggcggc aggttcatga ccagaagggc catttaaggt ttgtggccag
     2761 ccgttataaa acgcgctggg gcgagtatct atcgctttgc cattttgttg ctgaagttgg
     2821 tcaagtgtaa gaggcttcgc cagttcagcg gcccacgaag aagcgcaagc cagccctaaa
     2881 gccattgcca gcgcggtcat ttgagaaaca cgtttcatcg aaaatcccga cattatagtt
     2941 ttgagtgcgc cattctgaac tgacgcattg agaaaaactt tgcgttatcg cagtttgttg
     3001 taattttctg accactgcgc catatccaga acagaactat cagcaggaac atcctggaga
     3061 tcgtgcgtta cctgaacgac ggggatcgcc agggcgcgaa cttcgctgaa cacccactgg
     3121 cgaaaattat cgcgcagagc cacatcaaga cggctgaatg gctcatccag gagtaacgct
     3181 tttggctggg cgagaagggc gcgtagtaga gcaacgcgcg ctcgctgacc gccagacaaa
     3241 gtggcaggat cctgatggaa agctccgtca aggcctgaac gctcaagtgc atcattcacg
     3301 gcattacgtc gggcattccc cttaagtgtc gccggtagcg ccagcagtaa attttgcccg
     3361 acgctgaact ggtcaaataa cagtgcatcc tgaaaaagaa tgccaatctg acgctgtgcg
     3421 gtgggtagca tgtcaatccg ttgctcattg agccatagct cacctgtaca agaaaactgt
     3481 ccggccagtg caccaatcat ccatgaaaac agagtggatt ttccacagcc agatggcccc
     3541 attaacgtga cgatgtcacc tttatcaacc gtaaagttaa cgtttgtcag caagcggctt
     3601 tctggtaaac gtagcgaaac atttttcacg cagagcatta gcggattcct tgtctgacat
     3661 aacctaccca ttttgcgact aacgcggtca gggcaaaaat aataagcggt aacaacagtt
     3721 gccataaagc ctgggcggcg agaataccgt tgctgccgcc gctgcttaat gccaccgcct
     3781 cagtggtgag cgtcggaaaa cgccccgcgc ccagccacag cgtcggcata tactgcgcaa
     3841 tactgactga aaatcccacc gcgaaggcaa tcagcacagg gcgcaacatg agtgggcatt
     3901 tcacgtaaaa gaagattttg gcccgcgacc agcccagtgt ttgcgcaatc aaaattaacc
     3961 gtgaatcaat gcgctgccag gcgggttgca ggataaacag catccacggc attacccaca
     4021 gcagatgccc ccagaccacc gccgtccagc ttccatccag gttcagccat agcgccagcg
     4081 tgtactggcc cgccaccagt ggcagagcgg gcaatagaat gggcaaccat agccacaact
     4141 ggcgacgctg tgccccccat tccagccaca gcagtaacag gagcaaagcg atgaatgttg
     4201 ctaccagccc cattgtcaga ctgttgatga gtgcttcact attgatcgtc gactgatccg
     4261 cgaggatcgc cagcagaacc acacacagca caccggttaa gggtaaaaaa ctcgccagcg
     4321 tagtgcctgg taataaaggt gtggcaggct tgcgaatgcc atctacgcgg ggaatagtgc
     4381 gccgccagct gcgccatagc agatagctca gcaaaacgta ggcggcgagt aacagcatca
     4441 gcagcagact ggcgagtgcg cctttcgttt gttgatccgc gtcgccctga gttaaccact
     4501 gccagctaat tactgccagc gtcggtggat tacctggccc aagaataatc gccacatcca
     4561 cgaccgaaag cgaccaggca acaatcgcca gcatcgccat tgccagcgaa ggcgcgacgg
     4621 agggcaacag cagccagttc aggcattgcc agcggctgta gcccagcgaa tccagcacaa
     4681 tgacctgctg caacagccgt ttttcgctca atactgccac taagatccac agcagaaatg
     4741 cgctttcttt cactgcaagg gtgaggccca gaccgatcca aatcggtcca ttggcggagt
     4801 gaaatacggg aaatagtcat atagcagccc tccgtcagca aagagcagca gggcgctggt
     4861 ggcaaaagcc acatggggaa tggcgagcag ccacggcaga cgggcgcaca tacgctgcca
     4921 tttcggccca ggccatagcg ccacaatcac cagcagggca atcaacaatg ccccgaccgc
     4981 cacgatggtt gtcgacacca gcgttgccag taatgcctgc ggtaactgtg gatcggcaaa
     5041 taacgcctgc cagcgtgtca acgacaacgc aggcgatatc agcgtgagag ctgccgggat
     5101 cagcggtgca taaatcaccg ccaccatcgc ccacagcaaa aagattaatg cgtaccgtaa
     5161 cggcgtaacc attcttgttc cagtgcattc acccatcctg cgtgcggttc agccagtacc
     5221 ggcggcagat cctgcggcat tcttgattgc aatatttcgc gctgcccgtc aggcagtttt
     5281 tgcggatcga gaacagaagg atcgccccag acaacgggat ctgctttacg cagttgcgca
     5341 tcgggtgaga gcaggaaatt ggcaactacc ttcgccgcag cactggcatt cgcgttggct
     5401 ggaatggtga cgaaatgcac gttgccaatc atcccctcgc gaaagccaaa actgtaactg
     5461 cttgccggca aatcgccgct ggcgattttt tgctgcgcat gcgcggggtt aaaggtcagc
     5521 gacaggcgca atgtgccggc tttcagcaga gcatccatcc gcgcgggcga aggcgggaaa
     5581 tcctttcctt cgcgccacaa atacggatgc agtgcatcaa gatattgcca caagggcgca
     5641 gtgacacggg cgaaagtcgc atcgtccggc gcttctttta atgcggcggg atctggcgtc
     5701 agcataatca gcaactgttc aagaaacgcc gtgccggtaa agtccggtgg gcgcggataa
     5761 gtaaccgtgc cgggattggc tttagcaaac tccagtaagg cttgcggcgt ttgtggtggc
     5821 tgtggcgtaa catcgcggcg ggcgataaac gtcagttgtg cgccgcccca gggcgattcc
     5881 gcaccttgtg tcggcactga aaaatcttcc cgcaccggca gctgtgtgtc gacatagcgc
     5941 cagttgggca gagtctccgc ccagcccgtt tgtaataaat
//
LOCUS       DAJTYG010000163         5910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTYG010000163 DAJTYG010000000
VERSION     DAJTYG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.164"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..410
                     /locus_tag="OO568_003751"
     CDS             <1..410
                     /locus_tag="OO568_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6642896.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /gene="istB"
                     /locus_tag="OO568_003752"
                     /pseudo
     CDS             442..1056
                     /gene="istB"
                     /locus_tag="OO568_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="OO568_003753"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="OO568_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR6642897.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTSGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKTFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYTGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
     gene            5721..5909
                     /locus_tag="OO568_003754"
     CDS             5721..5909
                     /locus_tag="OO568_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642898.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
BASE COUNT         1448 a         1492 c         1410 g         1560 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggccagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tcaacagcgc aggagagctg
      721 ttgcaggaac tacataaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag gcctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggtata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggtc ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagta
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actgtacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gcccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc gacgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaagaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tgctcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aaggggcagt ttggatatcg agaaccacgg cagcctgacg gctgcaaatt gaaaaccgcc
     5641 cctgttcgtc ctgttgttgc cgcacgctcc gcagaatatg tatgctctga ggacctgttt
     5701 tattaaggac tcatcctctt atgttgaccc gcaaaagtat agataccgtt ctgctctctg
     5761 ttggtgctga gaagctctca caacgtgaat gggactggat gaagatgctc aaacccatgg
     5821 acccaccacc tgcgatggtc gcggcctcca tcctggagcg ccgtggcgac acagcagccc
     5881 ttacccggct acaggatacc gggggttaat
//
LOCUS       DAJTYG010000164         5809 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTYG010000164 DAJTYG010000000
VERSION     DAJTYG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.165"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            67..585
                     /gene="mddA"
                     /locus_tag="OO568_003755"
     CDS             67..585
                     /gene="mddA"
                     /locus_tag="OO568_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6642899.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            582..1031
                     /locus_tag="OO568_003756"
     CDS             582..1031
                     /locus_tag="OO568_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6642900.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1032..1264)
                     /locus_tag="OO568_003757"
                     /pseudo
     CDS             complement(1032..1264)
                     /locus_tag="OO568_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
     gene            complement(1350..1523)
                     /gene="ortT"
                     /locus_tag="OO568_003758"
     CDS             complement(1350..1523)
                     /gene="ortT"
                     /locus_tag="OO568_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6642901.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1718..1813
                     /gene="yncL"
                     /locus_tag="OO568_003759"
     CDS             1718..1813
                     /gene="yncL"
                     /locus_tag="OO568_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR6642902.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2215..3024)
                     /locus_tag="OO568_003760"
     CDS             complement(2215..3024)
                     /locus_tag="OO568_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR6642903.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3234..4659)
                     /gene="patD"
                     /locus_tag="OO568_003761"
                     /pseudo
     CDS             complement(3234..4659)
                     /gene="patD"
                     /locus_tag="OO568_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            complement(4681..5475)
                     /locus_tag="OO568_003762"
     CDS             complement(4681..5475)
                     /locus_tag="OO568_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6642904.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5465..>5809)
                     /locus_tag="OO568_003763"
     CDS             complement(5465..>5809)
                     /locus_tag="OO568_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6642905.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1522 a         1473 c         1290 g         1524 t
ORIGIN      
        1 ccgttgcttc gggctgtttt cgatagtatt gataacatca tcatcattat tttattgagg
       61 ccagacatgt ccatccgttt tgcccgcaaa gccgactgtg ctgccattgc ggaaatttat
      121 aaccacgccg tgttgtatac ggcggctatc tggaatgacc aaacggtgga tgcagataac
      181 cgcattgcct ggtttgaagc gcggaccata gcaggttatc cggtgctggt aagtgaagaa
      241 gatggcgtag ttacgggata tgcctcgttt ggcgactggc gtagtttcga tggttttcgc
      301 cataccgtgg aacattcggt ttatgtccat cccgatcatc agggcaaagg tctggggcgt
      361 aaattgttaa gccgattgat tgatgaagcg cgggattgcg ggaagcatgt catggtcgcc
      421 gggatcgaat cgcaaaatca ggcttcgctg catctgcacc agtcgctggg atttgtcgtc
      481 accgcacaaa tgccgcaggt aggcactaaa tttggtcgtt ggctggatct gacatttatg
      541 cagttgcaac tcgacgagcg cactgaaccg gacgcgattg gatgaatcag tcgctcaccc
      601 ttgctttttt gattgccgcc ggaattggtc tggtagtcca gaacacgctg atggtgcgca
      661 tcacccagac ctcctctacc attctcatcg ccatgttgct gaactcactg gtggggattg
      721 tgctctttgt ttctatttta tggtttaaac aaggcatggc ggggtttggc gaactggtgt
      781 ccagcgtgcg ctggtggaca cttatacctg gcttgctggg ttcgtttttt gtcttcgcca
      841 gtatcagcgg atatcagaat gtgggagcgg cgacaactat tgcggtactg gtcgccagcc
      901 agctgattgg cggtctggtg ctggatatct tccgcagtca tggcgtacca ctacgggcgt
      961 tgttcggccc aatatgtggc gcaattttac ttgtggttgg cgcctggttg gtggccagac
     1021 gctcatttta atcagaggat ggtgccgcct ttggttaact gttcgtggcc gcttccatat
     1081 cttctttata cttgcgaccg tgatgagcaa tagcggtgcg caaacgctgc tgctgggtgt
     1141 agcgatcttc ccgacttaac tccgcgtcat cgctcagggc gattaataat tcgttcatat
     1201 gggcaatgac gctctcttca atcgcggcat ccacgcgcgc gatgacttca tccagatgcg
     1261 acatactttc tccttagcca cttgtgaaat ttatcgatta atccaaatga tataagtctg
     1321 cgtggtgccg tcaagccagt gagtaagatt taaaacagtg aaaacaacag cgccaccggg
     1381 aaactcatcg gccatgtggc ccccaccaga aaggcgctta agaaacggat gcgtttctta
     1441 tcgtgagaaa gaaaccaggt gataagaaat gcgattgctg ccataaccgc ataaaaaaca
     1501 agcatatgtt gatagagaga catgtacggg cgtccagcca tatgaataaa acgcgcgcaa
     1561 atatgcgcca ttttttgacg tgcatcaagt tttttcacca gtttgagaaa aaatcatcaa
     1621 acagcaactt gcccttatcc atcttcagaa tgagtattat tgcggatttt cttaacccgt
     1681 actatacaca taggggcaat gataaaaggt ggcaaaaatg aatgtttcca gtagaactgt
     1741 agtactgata aatgtctttg ctgctgttgg tttgtttact cttatctcta tgagatttgg
     1801 ctggtttatt tgatgtagtg attttgcacg cagcacgtac tgtcagagaa atcaactctc
     1861 tgacagtacg caagatcttc tcatactctc caagtttgca acattatcct taatatttac
     1921 ttctttttgc tggcatctac attgaaatga gtagaaaaca tccccctact gtcgtctata
     1981 caaacagctt actgattcaa acggaatctt gttttcacat ttttctatca cgaatacttt
     2041 atacgcgcgg gagtctataa atgctttata ctttcaagga ataacgtaaa tataaaaaga
     2101 tggcgtaaag tatatgcatg gcaggatgtt tgcaacctcc ttgatgacgc tgccccattg
     2161 aaattgcaaa ccccgccctg gttatcttgc ccatgtatca tgcaaaacag ataattatag
     2221 gcctctgaat acctcagaaa gattgaattt tttatagtcc ctctcttgaa tatttccatt
     2281 tgatatcaat cgattcatct cttcaacaaa ataatttata tcatcacact tgttattatc
     2341 cggaagatct gagtagttat aattccacca ttttattctc aacagttcat caataacatt
     2401 tgaatcaaac ctaaatctga ttatttttgc aggaactcct gcaactatgg catatggggg
     2461 cacatctttg gtaacaacgg aattggcagc aataacagca ccatcaccta ttgcgatgcc
     2521 acctttcaat acaacatcat ttcctatcca gacatcattt cctataatcg gattttcagg
     2581 tttggtaatg aatggtttca atgtccagct acgttcaaat tcactcatag caaacttatc
     2641 aaattcaccg ttataagtca tcatgtgagt agtaaaacga ttcaaaggat gatttccacc
     2701 cattattttt acatttgatg caatactaca aaatcgacct atacgtatat tccctgaaaa
     2761 atggcaggtc ggaaatgaaa aagcgccgac actggagaaa tttttcacag gcattgttga
     2821 atattcttca gcaataacat tatcttcaat tgttatcttg tcaccttttg agaaaagaaa
     2881 taaatttctt tctttatacg acttcttccc cttatgattt aaaaagattt tattatccat
     2941 acaaaactca aagtgttttt tcgtccatct gaaggtaagg gttttatttt tatcttccgt
     3001 gaatttgtac aattgaactt tcattgttaa tacttctact tataaattat tagtttaata
     3061 acgatactct aaagccttat gctaatcaac tgtgacagga tgaaaaatcg ccatcaaaca
     3121 tttagcctaa tttttacgcc agttattaat gtaatcattc taatacttaa ccgccattaa
     3181 actcagaacc taaaacgata gtggccgtcc tataacagcc actaacatct aacttaatgt
     3241 ttaaccatga cgtggcggac gacggtgtaa tcctccagcc cataaagtga catatccttg
     3301 ccgtaaccag aaagtttctg cccaccgtgc ggcatttcac ttaccagcat gaaatgggta
     3361 ttgacccagg tacaaccata ttgcagccgt gcgctgacgc gatgcgccct gcccacatct
     3421 ttcgtccata ccgaagatgc aagtccgtac tggctgtcat tcgcccagtt caccacctgt
     3481 tcttcgttgt cgaagggcgt aacactcact actggaccaa atacctcttt ttgcacgatg
     3541 gcatcgtcct gtaatgcgcc agccagcagc gtcggcgcat agtaatagcc attacccttg
     3601 cgcttttcac cgccagtgat cactttgatg tgccctgtcg ctttcgcctc ttctactgcc
     3661 ttgctgacgc gttcgagatg cgccagcgag cttaaaggtc caagctccgt agactcgtca
     3721 tccggcgcac cagattttaa cgttgccacc gcaggcaccc agtttttcca ccagcgtatc
     3781 gtaaatgcct ttttgcgcgt agatccgaca agccgcagta caatcctgtc cagcattgta
     3841 atagccaaat gtacgtacac cttcgaccac tgcttcaata tccgcatcat caaaaacaat
     3901 cactggcgct ttgccaccaa gttccatatg agtacgctta atggacgacg cggtatggct
     3961 gatgatgtgc tcgccggtgg cgatagagcc cgtcagcgac accatccgca ctttgggatg
     4021 accggtcagc ggatcaccca ccgttttgcc tctgccaaac agtatgttaa tcacgcctgc
     4081 cgggaagata tctttcgcca gctctgccaa cttcaacgcg gtcagcgggg taatttctga
     4141 tggtttaagc actacgcagt tccctgccgc cagcgccgga gcaagtttcc acgcggccat
     4201 catcagcgga taattccacg gtgcgataga agccacgacc cccaacggat cgcgacggat
     4261 catcgaagta tgaccttcaa aatattcacc tgccgccaga ccattcagac agcgcgccgc
     4321 acccgcgaaa aagcgaaaaa catcgacaat cgccgggatt tcatcattga acgcactatg
     4381 cagcggtttg ccacaattac gggattccag ttcggcaaaa acctgaccat tttcttcgat
     4441 aacatccgcc agtttcagca ggcattccgc acgcgctttc ggtgtggttt gcccccattc
     4501 agcaaatgct gcatctgccg cacgcacagc agcatcgacc tgctctgcgg atgcctcggc
     4561 aatctccagt aaaacgtccc ccgttgccgg attatagaca ggctgttttt ccccttcgcc
     4621 gctaaccagt tctccgttaa tcagtaactt atgttgcata gattattcct gtatcagtgg
     4681 ttattgacca ttgtcgcctt cgcgggttag ccaccaggcc cccaggatcg gcaaggttgt
     4741 taccaacata accagcagtg ccaccacgtt ggttaccggt acatcacgcg gtcgcccaag
     4801 ctgattgagc aaccacaacg gtaacgttcg ttcatgacct gccgtaaagg tcgtaacgat
     4861 gatttcatcg aacgacaagg caaacgccag cattcctcct gccagtaacg ccgaactgag
     4921 attcggcaac actacgtagc ggaaggtttg ccagccattg gccccaagat ccattgacgc
     4981 ctcaaccaga ctccacgagg tgcggcgaaa acgggcgatg acattgttaa acaccaccac
     5041 tacgcaaaaa gtcgcatgac cgaccacgat ggtgaaaaat cccggctcca gattgatggt
     5101 tttaaaggcg gttaatagcg ccagaccagt gacaatgccc ggcagcgcaa tgggcagcag
     5161 caataacagc gaaatggcgt ttttgccgaa aaagtctcgt ctccacagcg cggcagctgc
     5221 cagcgtccct aacaccagcg caattaatgt cgccagcgcc gccactttaa gtgacagcgt
     5281 cacggcatca agaatatcac tacgctgtgc tgccacgcta aaccagcgca gcgtcaggcc
     5341 ctgcggtgga aaactaaacg ccgcatcttc agtgttaaac gcataggcgg cgattatcag
     5401 gatgggaaaa tgtaggaaaa caacgccgcc ccaggccgcc agtttgagga aaaacggtgc
     5461 gcgttcagag tgcatcgaac gctcccagac gtttcacgaa cgccaggtac agtgcgatga
     5521 gaataatcgg caccagggtg aatgccgccg ccatcggcat attgccaatc gccccctgct
     5581 gggaataaac catattgccg ataaaatatc ctggaggacc aaccagctgc gggacgataa
     5641 aatcgcccag tgtgagtgag aaggtaaaga tagagccagc ggcaataccc gggattgcca
     5701 gcggcagcac cacatagcga aaggtttgtc gtggacgtgc gccgagatca gccgacgcct
     5761 gcaacaatga cggcggcaaa cgctcaagcg ccgcctgaac gggcaggat
//
LOCUS       DAJTYG010000165         5809 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTYG010000165 DAJTYG010000000
VERSION     DAJTYG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.166"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            34..393
                     /locus_tag="OO568_003764"
     CDS             34..393
                     /locus_tag="OO568_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6642906.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(513..692)
                     /locus_tag="OO568_003765"
     CDS             complement(513..692)
                     /locus_tag="OO568_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6642907.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            766..2127
                     /gene="pabB"
                     /locus_tag="OO568_003766"
     CDS             766..2127
                     /gene="pabB"
                     /locus_tag="OO568_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6642908.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2131..2709
                     /locus_tag="OO568_003767"
     CDS             2131..2709
                     /locus_tag="OO568_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6642909.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            2893..4257
                     /gene="sdaA"
                     /locus_tag="OO568_003768"
     CDS             2893..4257
                     /gene="sdaA"
                     /locus_tag="OO568_003768"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6642910.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4388..>5809
                     /locus_tag="OO568_003769"
     CDS             4388..>5809
                     /locus_tag="OO568_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6642911.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPEATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAE"
BASE COUNT         1404 a         1454 c         1466 g         1485 t
ORIGIN      
        1 cgtgccatat cgttataatc cctttgatat tagatgcaaa ttaaggtcat atatagcctt
       61 atcgataaca tggttaattt taaggataag aatatgcctg ctgtaataga taaagccctg
      121 gatttcattg gtgccatgga tgtatcagcg ccaacaccaa gttcgatgaa tgaaagcacg
      181 gcgaagggaa tctttaaata tttaaaagaa ctgggagtac ccgcgagtgc cgctgatatt
      241 acggcgcgag ctgaccagga aggctggaac cctgggttca cggaaaaaat ggttggatgg
      301 gcaaaaaaaa tggagacagg tgaacgctct gtgattaaaa atcctgaata cttttcaaca
      361 tatatgcagg aagaactaaa agcactggtc tgagttaaat ttatatcagc ataaatgggt
      421 caggacgctt ttaatcacat ataaaaagcg tcctttttct cacaatcaaa tgatcatcgg
      481 cttctataac gaaatcgata cggattagtg tgttactcgt cttcatcctc aaaacgcgcc
      541 acgattagct cgccggagtg gttggcacgc agctcttctg ccaccagcgc gattgcctgg
      601 ccgctactca tcccttgtgc catcagttcc tggatccgct cgacagcttt ttgctgctgt
      661 tcatgggtga gtgaaggtaa acctgcaaac attgttaact cctgctaaat tgttggcgct
      721 aattatttca tgctacccgg cacatagcca gtagagtcag gactgatgaa gacgttatct
      781 cccgctgtga ttactttacc ctggcgtcag gacgccgctg aattttattt ctcccgctta
      841 agccacctgc cgtgggcgat gcttttacac tccggctatg ccgatcatcc gtatagccgc
      901 tttgatattg tggtcgccga tccgatttgc actttaacca ctttaggtaa agaaaccgtt
      961 gttagtgaaa gcgaaaaacg cacaacgacc actgatgacc cgctacaggt gctccagcag
     1021 gtgctggatc gcgcagacat tcgcccaacg cataacgaag atttgccatt tcagggcggc
     1081 gcactggggt tgtttggcta cgatctgggc cgccgttttg agtcactgcc agaaattgcg
     1141 gaacaagata tcgttctgcc ggatatggca gtgggtatct acgattgggc gctcattgtc
     1201 gaccaccagc gtcatacagt ttctttgctg agtcataatg atgtcaatgc ccgtcgggcc
     1261 tggctggaaa gccagcaatt ctcgccgcag gaagatttca cgctcacttc cgactggcaa
     1321 tccaatatga cccacgagca gtacggcgaa aaatttcgcc aggtacagga atatctgcac
     1381 agcggtgatt gctatcaggt gaatctcgcc cagcgttttc atgcgaccta ttctggcgat
     1441 gaatggcagg cattccttca gcttaatcag gccaaccgcg cgccatttag cgctttttta
     1501 cgtcttgaac agggtgcaat tttaagcctt tcgccagagc ggtttattct ttgtgataat
     1561 agtgaaatcc agacccgccc gattaaaggc acgctaccac gcctgcccga tcctcaggaa
     1621 gatagcaaac aagcagaaaa actggcgaac tcagcgaaag atcgtgccga aaatctgatg
     1681 attgtcgatt taatgcgtaa tgatatcggt cgtgttgccg tagccggttc ggtaaaagta
     1741 ccagagctct tcgtggtgga acccttccct gccgtgcatc atctggtcag cactataacg
     1801 gcgcgactac cagaacagtt acacgccagc gatctgctgc gcgcagcttt tcctggtggc
     1861 tcaataaccg gggctccgaa agtacgggct atggaaatta tcgacgaact ggaaccgcag
     1921 cgacgtaatg cctggtgcgg cagcattggc tatttgagct tttgcggcaa catggatacc
     1981 agcattacta tccgcacgct gactgccatt aacggacaaa tatactgctc tgcgggcggt
     2041 ggaattgtcg ccgatagcca ggaagaagcg gaatatcagg aaacttttga taaagttaat
     2101 aagatattac gccaactgga gaagtaaaac gtggaatacc gtagcctgac gcttgatgat
     2161 tttttatctc gctttcaact tttgcgcccg caaattaacc gggaaaccct aaatcatcgt
     2221 caggctgccg tgttaatccc catcgtccgt cgaccacaac cggggttgtt gctgactcag
     2281 cgttcaattc atctgcgtaa acacgctgga caagtggcat tccctggagg tgcagtcgat
     2341 gacacggacg catcagttat cgccgccgcg ctgcgcgaag ctgaagaaga ggtcgctata
     2401 ccgccttccg ccgttgaagt tatcggcgtg ctgccgcccg tcgatagcgt aactggctat
     2461 caggtaaccc cagtggtcgg cattatcccg cccgatctgc cgtatcgcgc cagtgaagat
     2521 gaagtctcgg cggtgtttga aatgccgctc gcccaggcat tacatctggg tcgttatcac
     2581 cctttagata tctaccgccg tggcgattca catcgggtat ggctgtcctg gtacgaacag
     2641 tattttgtat ggggaatgac cgcaggcata attcgtgagc tggcgctgca aattggtgtg
     2701 aacccctgac tatacttatc tttacatcta caaaacacta cttgagacaa tcatcgcaat
     2761 attagttaaa tcgcggtttt tgattagttt aattcatgtg aatagttaag ccagtcgccg
     2821 cgttccctct tacactatgc gctgttatta gttcgttact ggaagtccag tcaccttgtc
     2881 aggagtatta tcgtgattag tctattcgac atgtttaagg tggggattgg tccctcatct
     2941 tcccataccg tagggcctat gaaggcgggt aaacagttcg tcgatgatct ggtcgaaaaa
     3001 ggcttactgg atagcgttac tcgcgttgcc gtggacgttt atggttcact gtcgctgacg
     3061 ggtaaaggcc accacaccga tatcgccatt attatgggtc ttgcaggtaa cgaacctgcc
     3121 accgtggata tcgacagtat tcccggtttt attcgcgacg taaaagagcg cgaacgtctg
     3181 ctgctggcac agggacggca tgaagtggat ttcccgcgcg acaacgggat gcgttttcat
     3241 aacggcaacc tgccgctgca tgaaaacggt atgcaaatcc acgcctataa cggcgatgaa
     3301 gtcgtctaca gcaaaactta ttattccatc ggcggcggtt ttatcgtcga tgaagaacac
     3361 tttggtcagg atgctgccaa cgaagtgagc gtgccgtatc cgttcaaatc tgccaccgaa
     3421 ctgctcgcgt actgtaatga aaccggctat tcgctgtctg gtctcgctat gcagaacgaa
     3481 ctggcgctgc acagcaagaa agagatcgac gagtatttcg cgcatgtctg gcaaaccatg
     3541 caggcatgta tcgatcgcgg gatgaacacc gaaggtgtac tgccaggccc gctgcgcgtg
     3601 ccacgtcgtg cgtctgccct gcgccggatg ttagtttcca gcgataaact gtctaacgat
     3661 ccgatgaatg tcattgactg ggtaaacatg tttgcgctgg cagttaacga agaaaacgcc
     3721 gccggtggtc gtgtggtaac tgcgccaacc aacggtgcct gcggtatcgt tccggcagtg
     3781 ctggcttact atgaccactt tattgaatcg gtcagcccag acatctatac ccgttacttt
     3841 atggcagcgg gcgcgattgg tgcattgtat aaaatgaacg cctctatttc cggtgcggaa
     3901 gttggttgcc agggcgaagt gggtgttgcc tgttcaatgg ctgctgcggg tcttgcagaa
     3961 ctgctgggcg gtagcccgga acaggtttgc gtggcggcgg aaattggcat ggaacacaac
     4021 cttggtttaa cctgcgaccc ggttgcaggg caggttcagg tgccgtgcat tgagcgtaat
     4081 gccattgcct ctgtgaaggc gattaacgcc gcacggatgg ctctgcgccg caccagtgca
     4141 ccgcgcgtct cgctggataa ggtcatcgaa acgatgtacg aaaccggtaa ggacatgaac
     4201 gccaaatacc gcgaaacctc acgcggtggt ctggcaatca aagtccagtg tgactaatac
     4261 ttcttactcg cccatctgca acggatgggc gaatttatac ccgctttctc gtctgctgta
     4321 atattcccca ctacacttcc actgttgcgt caggcgtttg tcgccatacg cttacagggt
     4381 ggcccgcatg caaaaagcac aacggatcat taaaacctat cgccgtaatc gaatgattgt
     4441 ttgtacgatt tgcgcactcg ttacgctcgc ttcgaccctg agcgtgcgat ttatttcaca
     4501 gcgtaactta aatcaacaac gggtagtaca attcgccaat cacgctgtag aggaattaga
     4561 taaagtactg cttcctctac aggcaggtag cgaagtcttg cttccgctga ttggtctgcc
     4621 ctgctctgtc gcccatttgc cattacgtaa acaggcggca aaactccaaa ctgtgcgatc
     4681 cattggcctg gtgcaagacg gcacacttta ttgctccagc atttttggtt atcgcaatgt
     4741 gcccgtcgtg gacattctgg ctgaacttcc tgcaccgcaa ccacttttac gcctgacgat
     4801 cgaccgtgcc ctgattaaag gcagtccggt tttgattcaa tggacgcctg cagcaggcag
     4861 tagcaatgct ggggtcatag agatgattaa catcgactta ctgacggcaa tgctccttga
     4921 gccacaactg ccgcaaatca gtagcgccag cctgacggtg gacaaacggc atttgctcta
     4981 tggtaatggg ctggtagatt cccttccgca acctgaagac aatgaaaact atcaggtttc
     5041 ttcgcaaagc tttcctttta ccattaacgt aaatggtccg gaggctacgg cgctggcatg
     5101 gcactatctt ccaacacaat taccgctggc ggtgctgcta agtttactgg tgggctatat
     5161 cgcctggctg gcgaccgctt accggatgag cttttcccgc gaaattaatc tgggcctggc
     5221 gcaacatgag ttcgaattgt tctgtcagcc tttgcttaat gcgcgcagcc agcaatgtat
     5281 tggtgtagag attttgctgc gctggaataa tccgcgtcag ggctggattt caccggatgt
     5341 gtttattcct atcgcggaag aacatcattt aattgtgcca ctgacccgct atgtgatggc
     5401 agaaaccatt cgtcagcgcc atgttttccc gatgagtagt cagtttcatg ttggcattaa
     5461 cgtcgcaccc agccattttc gccgtggtgt gctgataaaa gatctcaatc agtactggtt
     5521 tagcgctcac ccgattcagc aactgatcct cgaaatcacc gaacgcgatg ccttactgga
     5581 tgttgattat cggattgccc gcgagctgca tcgtaaaaac gtcaaactgg cgattgatga
     5641 cttcggcacc ggcaacagct cgttttcctg gcttgaaaca ttacgtcctg acgtgctgaa
     5701 aattgataag tcatttaccg cagctatagg ttctgacgcg gttaactcga cggtgaccga
     5761 tatcatcatc gcgctggggc aaagactgaa tattgaactg gtggcggag
//
LOCUS       DAJTYG010000166         5698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTYG010000166 DAJTYG010000000
VERSION     DAJTYG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.167"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            55..1515
                     /locus_tag="OO568_003770"
     CDS             55..1515
                     /locus_tag="OO568_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6642912.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVAEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1551..1754)
                     /gene="ydfZ"
                     /locus_tag="OO568_003771"
     CDS             complement(1551..1754)
                     /gene="ydfZ"
                     /locus_tag="OO568_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6642913.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1932..2618)
                     /gene="ydfH"
                     /locus_tag="OO568_003772"
     CDS             complement(1932..2618)
                     /gene="ydfH"
                     /locus_tag="OO568_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6642914.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSNWFSEE"
     gene            complement(2707..3453)
                     /gene="ydfG"
                     /locus_tag="OO568_003773"
     CDS             complement(2707..3453)
                     /gene="ydfG"
                     /locus_tag="OO568_003773"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6642915.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3590..5635
                     /gene="dcp"
                     /locus_tag="OO568_003774"
     CDS             3590..5635
                     /gene="dcp"
                     /locus_tag="OO568_003774"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6642916.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1426 a         1353 c         1475 g         1444 t
ORIGIN      
        1 tttattcata taacaaaaca tatagcttgc catatttaaa tttaaggaat tgttatggga
       61 aataatttgt tatcagcaaa agcgacactc cctgtttatg atcgtaataa cctggcccca
      121 agaattgttc atttaggctt tggtgcattt caccgtgcgc atcagggcgt gtatgccgat
      181 attcttgcta cagaacattt cagtgactgg ggatattatg aggtcaactt aatcggcggc
      241 gaacagcaaa ttgccgattt acaacagcaa gataatcttt ataccgttgc ggaaatgtcg
      301 gccgatgcgt ggacggctcg cgtcgttggc gtcgttaaaa aagccttgca cgtacagatg
      361 gatggcttag aaaccgtgtt ggctgcgatg tgtgaaccgc aaatcgcgat tgtctctctg
      421 acaatcaccg aaaaagggta tttccactct ccggcgaccg gacagttaat gctcgatcac
      481 ccgatggtcg ctgccgacgt gcaaaatccc caccagccga aaacagcaac aggggtgatt
      541 gttgaggcgc tggctcgccg taaagcggca ggacttcccg catttaccgt catgtcatgt
      601 gacaacatgc cagaaaacgg tcatgttatg cgtgacgttg tcacttccta cgcgcaagcc
      661 gttgatgtaa aactggcaca atggatcgaa gataacgtga ctttcccatc aacaatggtg
      721 gaccgtattg tgcccgcagt ggcagaggat acgctggcga aaatcgaaca acttaccggt
      781 gtgcgcgatc ctgctggcgt tgcctgtgaa cctttccgcc agtgggttat cgaagataac
      841 tttgtagccg gacgtccgga atgggaaaaa gcgggagccg aactggttag tgatgtgctg
      901 ccttatgaag agatgaagtt gcgcatgctc aacggcagtc attcattcct ggcatatctg
      961 ggttatcttg ctggatatca gcacattaat gactgtatgg aagatgaaca ttatcgtcat
     1021 gcggcgtatg gcatgatgtt gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat
     1081 ttgcaagatt acgctaaccg attaattgca cgctatagca acccggcgtt acgtcatcga
     1141 acctggcaga ttgcgatgga tggcagccag aaattgccac agcggatgtt ggattctgtt
     1201 cgctggcatc tggcgcatga cagcaagttc gatctgctgg cgctgggcgt cgcgggttgg
     1261 atgcgttatg tcggtggtgt tgatgaacag ggaaatccga tagaaatcag tgacccactg
     1321 ttacctgtta ttcagaaggc tgtacaaagt agtgccgaag ggaaagcgcg cgtccagtca
     1381 ttgctggcga ttaaggcaat ctttggtgat gatttgccag acaatagctt gtttactgca
     1441 aaagtgacgg aagcgtactt gtctttatta gcgcatggcg cgaaagcgac cgtggcgaaa
     1501 tattccgtga agtaaaaaaa gcaatgccgc catcggcggc attcacacac ttagttcatg
     1561 ccgagacgaa tcaggtccag aggtgccagt ttctcttcac aaccttcaac aactacggtt
     1621 tttccgcggc ggatttgctc agcggtcaga ccttcagtat caatcgacag gatcttgcca
     1681 gtgtgaccgg tgccgctaac cataacacgg ctgccagtgg tgattgcgtt acggttacga
     1741 tcgtaggtcg tcataggtgt tttctccttt ctgatttaca gtcgaagccc acgtaacggg
     1801 cgcaatataa atactcctgt cgagtgaaat attttttgtt tttgatcaag ctcacatctt
     1861 ttttgatgca tgcatgagcc gtaaggcggg gggaaaagcc tctcatcccg gatgggatga
     1921 gagtaaatga attactcttc gctaaaccag ttgctgtttt cctggcggat ctggcgcacg
     1981 gattcgctga tttcctgcaa atgttgcgtc attgcacgtt ctaccgcatc gccatcacgt
     2041 ttttgcaggg cagagaaaat atcaagatgc tggcgtaaca gcatttctgg tggagaaaca
     2101 tggtcgaaac tcatatagcg cacgcgatca acggtcgctt tcaggttctc aatggtatcc
     2161 cacgccagtt gacagtcggc aatctgcgtc aggagttgat ggaagttgtc atcaagttca
     2221 aaaaaatcat ccagttgctt gcgctcaatg gcaatgcgtt gctggtgaag attttgttcc
     2281 agttggtagc actggctttc ggtaatcatg ctcgccgccc gacgcgccac cgcgcactca
     2341 atggcctgac ggataaaact gccgttgcgc acctgggcca tggaaatttt gttgacgtag
     2401 ctgccacgct gcggacgaat ttgaatcagg ccgttttccg ccagtttaat aaaggcttca
     2461 cgaaccggct ggcgtgacac attgaaacga acagaaactt ctttttccga caacggtgtg
     2521 cctggtgcaa tcaggcaatg gacaatgtcg cgacgaagaa tacgataaat ctgctgattg
     2581 acaggctgtg tgggattaag ttgcgtttcg acggtcattc gttcttgctt gtgagtgagt
     2641 taactgcatg agtctaccac ttttttgtga caagcaataa cccggcagtt acgccgggta
     2701 taaaaattac tgacggtgga cattcagtcc ggcatagctt tgggtaaccg gcatcatttc
     2761 cagggtattg atattgacgt gagcaggcag cgttgacacc caccagacgg cttcgctgac
     2821 atcttctggc gtcaatgcaa cggtattttg ataggttttt tccgctttac cgtcatcgcc
     2881 tttaaagcgg acattggaaa actcggtgcc acccaccaga cccggttcga tgtcggtgac
     2941 gcgcaccgcc gtaccatgca gatccgtacg cagattcagg ctaaactgac gaacaaacgc
     3001 tttcgtcgca ccgtaaacgt taccaccggc atacggccag ctgcctgccg ttgagccaat
     3061 gttaataata tgaccatgat tacgttcaac catacccggt aagacggcgc gcgtcatata
     3121 tactaggcct ttgttgttgg tatcaatcat cgtttcccag tcttcaacgc tggctttatg
     3181 cgcaggctcc atgcccaacg ccaggccggc attatttacc aggatatcaa tattgctcca
     3241 ctcggcaggg agcgatgcca gcatctcttc aatagcggcg cggttgcgaa cgtccagttg
     3301 ggcgatatac agattatctc ccagttcgtc ttttaactcc tgcaaccgct cctggcgacg
     3361 gccagtggcg ataactttat gcccttgttg aataaaacga cgagtaatgc attcaccaaa
     3421 acctgccgtt gctccagtta ctaaaacgat catctcactg ttcctcaacg cttttgtgtg
     3481 tcaataacat agcacgtgat gcttaagagc gggtaatact ggtttgcaag attgcactcc
     3541 gttatcagta agcctacgct atggaacacc tcgcgttcag gagaagaaaa tgacaacaat
     3601 gaatcctttc cttgtgcaaa gcacactgcc gtatctggct cctcattttg atcaaattgc
     3661 caatcatcac tatcgcccgg cattcgatga gggaatgcaa caaaagcggg cagaaattgc
     3721 tgccatcgcg cttaacccgc aaacgcctga tttcaacaat actattctgg cactggaaca
     3781 aagcggagaa ttaattaccc gcgttaccag cgtctttttt gcgatgactg cggcgcatac
     3841 caatgatgaa ttacagcgtc ttgatgaaca gttttccgct gaactggcgg aactggctaa
     3901 tgatatctat ctgaacggtg aattattcgc gcgggtagat gctgtctggc agcgccgtga
     3961 atccctgggg cttgatagtg aatccatccg cctggtggag gttattcatc aacgttttgt
     4021 ccttgccgga gccaaacttg cacaagctga taaagcaaaa ttaaaagtac tgaatacaga
     4081 agctgcgacc ctgaccagcc agtttaacca gcggttactt gcagcaaata aatccggcgg
     4141 tctggttgtg aacgatatcg cgcagctggc aggaatgagt gagcaagaga ttgcgctggc
     4201 ggcagaggcg gctcgcgaga aaggtctgga taacaaatgg ctgattccgc tgctgaatac
     4261 cacccaacaa ccggcgcttg ccgaaatgcg cgatcgtgcg acgcgtgaaa aactgtttat
     4321 tgcgggctgg acgcgagcgg aaaaaaatga tgccaatgat acccgcgcta tcattcaacg
     4381 tctggtagag attcgcgcac agcaggcgaa actgcttggt tttcctcatt atgccgcatg
     4441 gaaaatcgcc gatcagatgg caaaaacgcc agaagcagca ctcaacttta tgcgggaaat
     4501 tgttccagcg gcgcgtcaac gtgcgagcga tgaattagcc tccatacagg cggttatcga
     4561 taagcagcag ggcgggttta gcgcgcagcc gtgggactgg gcattttatg cggaacaggt
     4621 acggcgggag aaatttgatc ttgatgaggc gcagctcaag ccatattttg aattaaacac
     4681 ggtgttaaat gaaggtgtat tctggaccgc gaatcagctc ttcggtatta agtttgtcga
     4741 acgttttgat attccggtct accatcctga cgttcgtgtg tgggaaattt ttgatcataa
     4801 tggcgtggga ctggcgttat tttacggtga tttcttcgcc cgtgattcaa aaagcggcgg
     4861 tgcatggatg ggcaattttg ttgagcaatc aacgcttaat gaaacgcatc cggtaattta
     4921 taacgtctgc aattatcaga aacccgctgc cggtgagcct gcgttgttac tctgggatga
     4981 tgtaataacc ttattccatg aatttggtca tacgctgcac ggcctttttg cccgccagcg
     5041 ttatgccacg ctttccggca ccaacacgcc gcgtgatttt gtcgaatttc cgtcgcaaat
     5101 caacgaacac tgggcaacgc atccgcaggt attcgctcgc tacgcccggc attatcagag
     5161 cggggcagca atgcctgacg aactgcaaca gaaaatgcgt aatgccagcc tgttcaacaa
     5221 agggtatgag atgagcgaac tgcttagcgc cgcacttctc gatatgcgct ggcattgcct
     5281 ggaagaaaac gaagcaatgc aggatgtcga tgattttgaa ttgcgggcgc tggtggcgga
     5341 aaatatggat cttcctgcta taccgccacg ctatcgcagc agttatttcg cccatatttt
     5401 tggtggcgga tatgctgcag gttattacgc ttatctgtgg acgcaaatgt tggccgatga
     5461 tggttaccag tggtttgttg agcagggcgg attaacgcgt gaaaatgggc agcgttttcg
     5521 cgaggcgatc ctttccagag gtaacagcga agatctggaa cgcctgcatc gacaatggcg
     5581 cggtaaggct cctcagatta tgccgatgct gcaacatcgt ggcttgaaca tataaacacc
     5641 accaatggct ggcctggaga tatcgccagg ccagcctggt tatagcagta attgcagc
//
LOCUS       DAJTYG010000167         5649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTYG010000167 DAJTYG010000000
VERSION     DAJTYG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.168"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..1243)
                     /locus_tag="OO568_003775"
     CDS             complement(<1..1243)
                     /locus_tag="OO568_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6642917.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSL"
     gene            complement(1240..2118)
                     /locus_tag="OO568_003776"
     CDS             complement(1240..2118)
                     /locus_tag="OO568_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6642918.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(2129..3036)
                     /locus_tag="OO568_003777"
                     /pseudo
     CDS             complement(2129..3036)
                     /locus_tag="OO568_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(3023..3256)
                     /locus_tag="OO568_003778"
     CDS             complement(3023..3256)
                     /locus_tag="OO568_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642919.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(3253..4110)
                     /locus_tag="OO568_003779"
     CDS             complement(3253..4110)
                     /locus_tag="OO568_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6642920.1"
                     /translation="MSAQYIDSAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSC
                     HEHCYAQIMVGRAGASQDAPGSMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTL
                     PTLSQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILV
                     RQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADM
                     SSVAQNARGVYLHLREIHQIWTSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRL
                     NGSDKEVLP"
     gene            4135..4464
                     /locus_tag="OO568_003780"
     CDS             4135..4464
                     /locus_tag="OO568_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6642921.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4454..4762
                     /locus_tag="OO568_003781"
     CDS             4454..4762
                     /locus_tag="OO568_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6642922.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4804..4980)
                     /locus_tag="OO568_003782"
     CDS             complement(4804..4980)
                     /locus_tag="OO568_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCR6642923.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4977..5387)
                     /locus_tag="OO568_003783"
     CDS             complement(4977..5387)
                     /locus_tag="OO568_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR6642924.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(5384..>5649)
                     /locus_tag="OO568_003784"
     CDS             complement(5384..>5649)
                     /locus_tag="OO568_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642925.1"
                     /translation="RQFENWWSKDESQFTGDDELKEFAWVIWQAPRAAIELDIDWPES
                     NDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
BASE COUNT         1348 a         1515 c         1335 g         1451 t
ORIGIN      
        1 ccagagaacc gttacgttgt ttcgtgatat tgatttctgc gatgcccctg gcctgcgtat
       61 ccgggttgta cacttcatcc ctgtaaagca tcagaatgat gtctgcatcc gcctctattt
      121 ctccggaatt tttcaggtct gagttcatgg gacgtttatt gggtctggac tccacaccgc
      181 gggagagctg gctcagcgca atcaacggaa aaccaccgga ttttgccagg cctttaagcc
      241 cctttgagat ttcacccacg gcaaggtcat gacgccccgt ggttcgggtt tttatcagcc
      301 cgagataatc aaccaccacc agcgccgttt ccggatattt aatcaggtgg tgtttcgttg
      361 ttgcgcatat ctcatcaatg gccaggttcg cctggtccac catccagata ttgcgcccgg
      421 tcatccgccc caccccttgt gagaaacgcg tccagtcttc atcttcaaag tgagccacag
      481 atttcaggcg tgatactggc atccctccag ccgcagacac catacgttca ccaatctgga
      541 tgttcgccat ctccatggtg aacagaagca caccatgccc ctgctcagtc accttgtcga
      601 tgatgtccag cgcaagttcg gttttcccca tcgaaggacg ggcggcaatg aataccaggt
      661 ctccgggctc cataccgcct gtttttgcgt ccagttcatc aataccggtc atcaacgtcc
      721 tggatttctc cagcccctga ttccggcatt caacacgctc aaccacttcc ggaagcacat
      781 catcaatatg taccggctga atgacgccct ttcctgtcga cagtgtgacc atcatgttct
      841 gcgcatcctt cagggcatct tcagctgctt cacaggtatg cgcatcacgt aatttctgca
      901 gcgcctcatt cagtgttttt tctgcatcgc gcagtgcggc attgcgccgc aacgctgcaa
      961 catagtgctc cagtgaagac ttcacccagg ttttacgccc ggtatcagta atcaccgggg
     1021 caagttccgg catctcatta cacaacagca cggggtcaat cactcctgaa acacgggcct
     1081 gtctgcagat gcctgtatag atatcctgat acgctcgtac agaaaaaacg tccgctggca
     1141 gtgtggccag aatatccatc acttcaggat ctgctccgcg cagaaagaac gcgccgatga
     1201 cagcgccttc aaggtcatcg ttacgccata ctggagttgt catgcagcca cacctctgat
     1261 acgagaacgg taactgggcc agttaaacga caaccagttg cgtcccccgt ctgtgatcct
     1321 gtcggcaatt cggggactga tgaacgccca caactcttcc ggtgagaggt tactgatcag
     1381 aatggtgggc aagatacttt cgtacctggc gttgataatt tcctgcaaaa tagccatttc
     1441 agccgcgctg ccaaactgaa cgccaacttc gtcgatgatc agcaaatcca ttgacgcata
     1501 acgctcaata acttcatccg ctgttttttc gctgtcattc cgccagcaat ttttcacagc
     1561 acgggtaagg cgcatcacgt cggtgatctc cacactggcc agatagttac ggatgatgtg
     1621 ttttgccata gccacagcca gatgattttt tccggtacca caactgccgg tcataacaag
     1681 actggtaccg ttctccagca tatctggcca attctccgca tagcggcgac aggccgcaag
     1741 atttctggct gcgccaggat taacctccag ataattatca aactcgcagt cccgaaaacg
     1801 cagggcaatt ccggcgttat cagtcagctc ttccgccttg atggacgaca gctccatggt
     1861 caaatcgttg gcctcagcga ttaagcagtc agggcagcat gaaatttttt ctctgtcctc
     1921 gccattacga tcgctccaca ccagtatatg cgtgtgatat tcgccatgtt tttcgcaata
     1981 tccgcgacct tcacgcatca ggcaggaacg ataaggccat ggcttttcgc ccttctgagc
     2041 aaatgcaatc tctgcccgta actcatccat tcgcgcctgt agtctttttt gttgttcacg
     2101 caggttaaac gtcatcatcg ctgtcacctc agaatgtcag tttgtcactg gatttaccga
     2161 atttgtcaga catggctccc aggccagcca ggacatcgac ctgtcgctgt cgcccacctc
     2221 cgggagcggc tggctgttgc cagaagtctt cgaagtgacg atcgggtcca aagaacgtcg
     2281 cagcctgctt cacgaactgt gtgccggtat ttcctgtggc acgtacccag gcagcatagc
     2341 gtttcacgcc atcaagcatg gcttcaggtt ttattccctc cctgagccgg gctttccagg
     2401 ctttgaaggc tgcggatttt gagttaccac cagcacgttt tggatattcc tgccaggcct
     2461 gttcaaattc cggtgaatat tcctgtcggg aagaacgcgc tggtgcagac gcgtcagcgg
     2521 atgcgccaat agtgttttta ctctctgtag tattctctga agtaatctct gttgtattct
     2581 ctgtaagatc gaaattggtt ttcccttctc cgcggcgagg ggtttcccgt gtccgcggtg
     2641 aaggctttcc ctcctccgcg aaattgggtt ttacagtttc ccgaaaacgg gtttccccat
     2701 ttcgggaaac tgattgtttt cattgataat ttcattaagg cgctcacaat ctatacggta
     2761 gaacattttg tgctcaagac gcttgttggt ttcaaccaaa atgcctctgg acacaagatg
     2821 cttacgcgct acagcctgtt gttcaaatgt aagtccggtt tcgtgttgta tctcttcacg
     2881 cgttttatgt acgccttccg ctgcatgtgc tttatcctgc cagtaaaaaa tctgaccaaa
     2941 gaaaataaca gcgtgcggac ttcccatgta tttaacgagc ccagggtaat aagcaaccgg
     3001 atgtccaaaa tcgagcagaa gatcagacgg acgcatagcc acctcccagg cgtttaaaca
     3061 tttttccggg ctgaaacgcc accagcggat aactcagggt atgagtacgt ccctgaacct
     3121 ggcagacaac cttctggctt tcggtactga ccaggcaaac ccgcagaacg tagccgttgc
     3181 tggtggtgaa ccactgcccc acacgggggc aatggttgta tcggtgatac aggaaattaa
     3241 cgatgtggcg gatcatggga gcacctcctt gtcagaacca ttcagcctgg aatcaacaag
     3301 tgcagcacca aaaacagcat cacctacacg gtcgtacagt ttgccagcca gcggagattc
     3361 aacggcctta agcattgggt aaagctggct tgtccagatt tgatggattt cacgcaaatg
     3421 caggtatacg cctctggcgt tttgtgcgac agatgacatg tcagccgcac cagctcctga
     3481 taagctcctc tccatctggt taaaggcatt gatgtatgcc tctttgaacc gggcggcacg
     3541 tttaccagtg aagcccatag caaggaaggc gaagccgtcg cgggtaattt ggtagcaggg
     3601 aagtttgcga gtaccgccgt tgggctggcg taccaaaatt gatgtctccg caaaattgcg
     3661 ggcacaaaac tctggagaac aatccaaaat gcggatcttt ttcagaacat cgtcatgacg
     3721 tttagagaag aagttggcaa cagccaggga tgaagtaaca gcctgaccat caacgatggc
     3781 aatttcaggt tgagagaggg ttgggagagt agtcatggtg acagccccta tgttgaattc
     3841 aaagaactca ccacatggga cgccaatcac agaggtggtg agacgtacag ggttggcgta
     3901 accggtcaac atagaacccg gcgcatcttg cgatgcccct gcacgcccca ccataatttg
     3961 ggcgtagcaa tgctcatgac acgaaaaaac cgcatgagcg cggttatgct ctatattgaa
     4021 tttcaggacg ccaatcccgg cacccgcttt ataaggtgca gagacagtgt aacgtcccga
     4081 aattgcagaa tcaatatatt gtgcagatat aacgattttg ttatgttttg ctgtatgaac
     4141 tacactatcg aatactacag tgaagaggtc cgactggagg tcgatcagct tccattgagt
     4201 atgcgtgcca gataccagca tcttgttgaa cgcatgaagg tatacggcag caatctcgga
     4261 gaacctcaca ccagcgcctt tggtgacggg cttttcgaac tcagaattaa aggcaacgat
     4321 gggatcgcgc gtgtttttta ctgcactctg acagggaaac gcatcatcat gctgcatagt
     4381 tttgtaaaga aaacgcagaa aacaccgcca gccgaacgca agaaagctga aaccagaatg
     4441 aaggaggtta agcatgactg gtaaacgcac tctccccacc atgacacacg acgaaatggc
     4501 agccaaatgg atggaagacc cggcttttaa agcagaatac gacgctatcg ctgacgaatt
     4561 cgcactgctt gatgaaatgc tggcagcacg caaagaagct ggcttaactc aggctgaagt
     4621 tgccgagcga atgggaacaa aagcgaccgc gatcaccaga atggaaagta atctcgcatc
     4681 aggtatcagc ggcccatcat ttgccacact aaaaaaattc gcccgcgcta ctggaaaaaa
     4741 actccagatc cgcttcgttt aaccacaccg cgccgtcatt ctggcggcgc accggataaa
     4801 tagtcatttc ctcgcacgat gtcttagcca ccggatatcc cacaggtgag ccgtgtagtt
     4861 gaaggttttt acgtcagatt cttttgggat tggcttgcgt ttatttctgg agcgtttcgt
     4921 tggaaggtat ttgcagtttt cgcagatgat gtcggtgaaa cttcgtcgct gtcgcctcat
     4981 tcatacctcc tgtcggtaaa tctgacaccc tgaccaatag cccaggctgt tgtgtactcg
     5041 atcagacttg ccatgcgctt cacgctcatc tgcgcgctgc tttcgcgaat gttgacgcat
     5101 tctccttcaa ggccgggcaa aacatcagct tcctgttttg ttgccactgc atgaccgctg
     5161 atcaacaaaa ccttccattg ttctggtttt aaccatttgc cgcaccattg aacctgacga
     5221 gcgatatccg ccagcatcgc gtgaaatttt gcgttctggt caagatttcg cttgtagtca
     5281 gtaatgcgga tggtgactgg cttgtcttta tcgagaggag ttgagaggat ggcatttatt
     5341 gcggcttgct gttgttgctt agttcggagg aagattgttt gcttcatcga aaattcttct
     5401 ctttaattcc agcggctctg atagctttca ttactgcaat taccgttttg tccttcccat
     5461 cctcatgccc catcgcataa gcaccttctt caccatcttt ccaaaagtcg tcattcgatt
     5521 cgggccagtc gatatccagt tcaatagctg ctcgcggtgc ctgccatatc acccaggcaa
     5581 actcttttaa ttcatcgtct cctgtgaact ggctttcgtc ttttgaccac cagttttcaa
     5641 actgtcggt
//
LOCUS       DAJTYG010000168         5523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTYG010000168 DAJTYG010000000
VERSION     DAJTYG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.169"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            92..1000
                     /locus_tag="OO568_003785"
     CDS             92..1000
                     /locus_tag="OO568_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR6642926.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1046..3037)
                     /gene="tkt"
                     /locus_tag="OO568_003786"
     CDS             complement(1046..3037)
                     /gene="tkt"
                     /locus_tag="OO568_003786"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6642927.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3315..4073
                     /gene="loiP"
                     /locus_tag="OO568_003787"
                     /gene_synonym="yggG"
     CDS             3315..4073
                     /gene="loiP"
                     /locus_tag="OO568_003787"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6642928.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4279..5199)
                     /gene="speB"
                     /locus_tag="OO568_003788"
     CDS             complement(4279..5199)
                     /gene="speB"
                     /locus_tag="OO568_003788"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6642929.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IMGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5335..>5523)
                     /locus_tag="OO568_003789"
     CDS             complement(5335..>5523)
                     /locus_tag="OO568_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642930.1"
                     /translation="FQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCS
                     GQAFPADNNGYAPPPCRK"
BASE COUNT         1322 a         1481 c         1477 g         1243 t
ORIGIN      
        1 acccctttat agattctgaa ttctcaccta tcggctgttt tctcctcatc tcatgtgaca
       61 tattcccata ctttactcaa ggaagattgt catgaaagca cctgtttcat taacctcatt
      121 tcgcccacaa aaatctcttg ctatcgccat tggcgtgctt gccgtggttg tgctgccatt
      181 tctctcgtat tacaccgtta atgaaggtga acgcggcatt ttgctgcgct acggaaaaat
      241 cgtaaaagtt gccgagccgg ggctgggttt taagatccct tttatggaaa gcgtggagaa
      301 aatttccacg cgtaatcagg ctgtggtcta tcagggatta caggcctaca gccgcgacca
      361 gcaaccggca caaatgacag tatcagtcag cttccacatt aaaccttcag aagcaggcgc
      421 ggtttatacc acctacaaca ctattgaagc attaaaagat cgtctaattg tccgtcagtt
      481 gccaactcag ctggagaaca ttttcggcca atataccgcc atcagcgccg tacaggatcg
      541 tacaaaattg gttcaggatc tgcaaaatgc catgcgcaaa gccgttgttg ggccagtggt
      601 aattgacggg gtccagattg aaaatattga tttttctgac gcttatgaaa agtcgattga
      661 aaaccgaatg aaagcagaag tcgccattgc cacgcgtaag caaaatctgg aaacagagaa
      721 aatccaggcg caaatagcgg taactcaggc ccaggcagaa gcagacagta aactggcggc
      781 agccaaagca gaagcggaaa ctattcgcgt aagaggcgcg gcagaggctg aaactattcg
      841 tttaaaaagt gcggctgaag cagaagcaat ccgcctgcgc ggtgaagctc tgcgggataa
      901 tcccggactt gtagccctca ccactgccga acgttgggac ggtaaactgc ccgataccat
      961 gatccccggc agcacagttc cgtttatctc tactaagtaa aaaaagggcc gctttcgcgg
     1021 cccttctcac aaaacgtctt actgattaca gcagcgcttt tgctttcgca acaacgttat
     1081 caacagtgaa gccgaactct tcaaacagca gctctgccgg agcagattca ccgaaggtgg
     1141 tcatgccgac gatagcgccg ttcaggccaa catacttgta ccagtagtca gcaatacccg
     1201 cttctaccgc aacgcgtgca gtaaccgctt tcggcagtac ggattcacgg taagccgcat
     1261 cctgcttgtc aaatgcgtcg gtagacggca tggacaccac gcgcgctttc acgccttcgg
     1321 cagtcagttt ttcgtaggca gcaaccgcca gttcaacttc agaaccggtg gcgatgaaga
     1381 tcagttccgg ctgaccagca cagtctttca gcacataacc accgcgcgcg atgtttgcca
     1441 gctgctcttc agtacgttcc tgctgcgcca ggttctgacg ggagaggata agcgcagtcg
     1501 ggccgtcctg acgctcaacg ccgtatttcc acgcgaccgc ggattcaacc tggtcacacg
     1561 gacgccatgt agacatgttc ggggtcacgc gcagagaagc gacctgctct accggctggt
     1621 gagtcgggcc atcttcgccc agaccgatgg agtcgtgggt gtaaaccatc acctggcgct
     1681 gtttcatcag cgcagccata cgtacggcgt tacgtgcgta ttccacgaac atcaggaagg
     1741 tggaggtgta cggcaggaag ccaccgtgca gggagatacc gttagcaatc gcggtcatac
     1801 cgaactcgcg aacaccgtag tggatgtagt tacctgcagc atcttcgttg attgctttag
     1861 aaccagacca cagggtcagg ttagacggtg ccaggtcagc agagccgccg aggaattcag
     1921 gcaacagcgg gccgaacgct tcgatagcat tctgcgacgc tttacggctg gcgattttcg
     1981 ccggattagc ctgcagttta gcgataaact ctttcgcttt ggcgtcgaag tcagacggca
     2041 tttcgccttt catacggcgg gtaaattcag ccgcttcctg cggataagct ttcgcgtaag
     2101 ccgcaaactt ctcattccat gcagattctt tcgcctggcc tgcttctttc gcatcccact
     2161 gagcatagat ttcagacggg atttcgaacg gtgcatattt ccagcccagc tgttcgcggg
     2221 tcagggcaat ttcagcgtcg cccagcggcg caccgtggga gtcgtgagta ccggctttgt
     2281 tcggggaacc gaaaccgatg atggttttgc acatcagcag ggacggtttg tcagtcactg
     2341 cgcgcgcttc ttcaactgcg cgtttgatgg atgccgcgtc atgaccgtcg atgtcgcgaa
     2401 taacgtgcca gccgtaagct tcgaaacgca ttgctgtgtc gtcggtgaac cagccttcaa
     2461 cgtgaccatc gatagaaata ccgttgtcat cgtagaacgc aatcagttta cccagcttca
     2521 gcgtacccgc cagagagcaa acttcgtggg agatgccttc catcatgcag ccgtcgccca
     2581 tgaaggcgta ggtgtagtgg tcgacaatgt cgtggcccgg acggttaaac tgcgccgcca
     2641 gcgttttttc tgcaatcgcc ataccgactg cgttggcaat accctgaccc agcggaccgg
     2701 tggtggtttc cacaccagcg gtataaccta cttccgggtg gcccggagtt ttagagtgca
     2761 gctgacggaa gtttttcagt tcttccatcg gcagatcgta accggtgagg tgcagcaggc
     2821 tgtagatcag catggagccg tggccgttgg acagcacgaa gcggtcacgg tcagcccagg
     2881 acggattctg cgggttgtgt ttcaggaaat cacgccacag gacttcggca atgtcagcca
     2941 tacccatagg ggcacccggg tgaccggatt tggctttctg tactgcgtcc atgctcagcg
     3001 cacgaatagc attggcaagc tctttacgtg aggacatttt gactccagat cggatgatga
     3061 agggcacgcc cttaacgact tgacgacagc gcgttttggg ctacgccgga aaatttgcca
     3121 acaatttacc gcaagccgcg cgtcatgtac atggaacatc cttttgccgc ttcagaaatc
     3181 tctggatcat gctcgcatgt tgcgcaatct gctcgcccgt ccgctgcgct tttccttata
     3241 ctgagactga gcgtcgattc acctgcaaac ggcgcatttt tagaataatc ctgaccttgt
     3301 gcggaagaga aaacatgaaa attcgcgcct tattggtagc aatgagcgtg gcaacggtac
     3361 tgaccggttg ccagaatatg gactccaacg gactgctctc atcaggagcg gaagcttttc
     3421 aggcttacag tttgagtgat gcgcaggtga aaaccctgag cgatcaggca tgtcaggaga
     3481 tggacagcaa ggcgacgatt gcgccagcca atagcgaata cgctaaacgt ctgacaacta
     3541 ttgccaatgc gctaggcaac aatatcaacg gtcagccggt gaattacaaa gtgtatatgg
     3601 cgaaggatgt gaacgccttt gcaatggcta acggctgtat ccgcgtctat agcgggctga
     3661 tggatatgat gacagataac gaagtcgaag cggtgatcgg tcacgagatg gggcacgtgg
     3721 cgttaggcca tgtgaagaaa gggatgcagg tggcgcttgg tacaaatgcc gtgcgagtag
     3781 ctgcggcctc tgcgggcggg attgtcggca gtttatctca atcacaactt ggtgatctgg
     3841 gcgagaaatt agtcaattcg caattctccc agcgccagga agcagaagcc gatgattatt
     3901 cttacgatct tctgcaccaa cgcggcatca gcccggcagg tcttgccacc agctttgaaa
     3961 aactggcgaa gctggaagaa ggccgtcaaa gctcaatgtt tgacgaccat cctgcatccg
     4021 ccgaacgtgc ccagcatatt cgcgatcgca tcagcgcgga tgggattaag taaagcctgg
     4081 tggtgtcgaa cgtaggtcag ataaggcgtt cacgccgcat ctgacattaa tggcacattt
     4141 gttaccttgt gcgcattgcc ggatgcgatg ctggcgcatc ttatccggcc tacgggtgcc
     4201 gaacgtaggt cggataaggc gttcacgccg catccgacat taatggcacg ttttacccgt
     4261 gcgcatcgca tctggtgctt actcgccctt tttcgccgcc tgaatataca gcatctccag
     4321 cgccagcgtc gccgcagcca gagcggtgat ttccgactga tcgtatgccg gagccacttc
     4381 cactacgtcc atcccaacga tgttgagatc tttcaggccg cgtaccagtt taatggcgcg
     4441 atcggaggtc aggccgccaa tcactggcgt accggttccc ggcgcgaaag caggatccag
     4501 gcagtcgata tcaaaagtca ggtaaaccgg catatcaccc ataatctgtt tcacctgggc
     4561 gataacgtca tccacgctgc gatcgttcac ctggcaggcg tccagcacgg taaagccgtt
     4621 gtctttatca aactcggtac gaataccaat ctgcacggaa tgattcgggt cgatcagacc
     4681 ttctttcggc gcggtataga acatagtgcc gtggtcaaat tcacaaccgt tcgcataggt
     4741 atcggtgtgg gcgtcaaagt gtaccagcgc cattttgccg aaatgcttcg catgagcacg
     4801 cagcagcggc agcgtaacaa agtggtcacc accgaaggag agcatacgct taccggcagc
     4861 cagcagcttc tcggcgtgcg cctgcagctt ttcgctcatc tcacgggcat cgccaaaggc
     4921 atataccaga tcgccgcagt ccacgacgtt cagacgctca cgcatgtcga aattccacgg
     4981 gaagcggttg tgttcccagg ccagattcgt cgaaacctga cggatcgctg ccggaccgtg
     5041 gcgaccaccc gcacgaccag aagtggccat atcgaacggc acgccagtaa tcacccagtc
     5101 tgcatcgctg tcatacggct ggaagttcat cggcaggcgt aaaaaaccaa aggcattgga
     5161 aaccagtgag ttatcgtatt gatgacctaa ggtgctcatg gatggactcc tattacaaag
     5221 tcgatataaa aaaacccttc cgcgtcgtta ggcccgacga ggaagggttg gatttgcgaa
     5281 acacaagttt gtgctaatta tcgccttaaa tgggcgaggc atcaagcgag gttgctattt
     5341 ccgacacggt ggcggcgcat aaccattgtt atctgccgga aaggcttgtc cagagcacgg
     5401 cggcagcgcg ttttgcgccg ggccttgtaa ttcaaaggcg gcaaagccga ttaccccggt
     5461 attacgcaca tcgccttgcg ccgaattggc tgcgtaggaa tcagcaacgt tggcgaactg
     5521 aaa
//
LOCUS       DAJTYG010000169         5486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTYG010000169 DAJTYG010000000
VERSION     DAJTYG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.170"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            2..109
                     /locus_tag="OO568_003790"
     CDS             2..109
                     /locus_tag="OO568_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport component
                     CsgF"
                     /protein_id="HCR6642931.1"
                     /translation="MANRNGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            136..911
                     /locus_tag="OO568_003791"
                     /pseudo
     CDS             136..911
                     /locus_tag="OO568_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(975..1466)
                     /locus_tag="OO568_003792"
     CDS             complement(975..1466)
                     /locus_tag="OO568_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6642932.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1568..2122)
                     /gene="ycdY"
                     /locus_tag="OO568_003793"
     CDS             complement(1568..2122)
                     /gene="ycdY"
                     /locus_tag="OO568_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6642933.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(2146..2883)
                     /gene="ycdX"
                     /locus_tag="OO568_003794"
     CDS             complement(2146..2883)
                     /gene="ycdX"
                     /locus_tag="OO568_003794"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6642934.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(2938..3876)
                     /gene="ghrA"
                     /locus_tag="OO568_003795"
                     /pseudo
     CDS             complement(2938..3876)
                     /gene="ghrA"
                     /locus_tag="OO568_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            4110..4197
                     /locus_tag="OO568_003796"
     tRNA            4110..4197
                     /locus_tag="OO568_003796"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4144..4146,aa:Ser,seq:gga)
     gene            complement(4390..5376)
                     /locus_tag="OO568_003797"
     CDS             complement(4390..5376)
                     /locus_tag="OO568_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642935.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFLWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(5373..>5486)
                     /locus_tag="OO568_003798"
     CDS             complement(5373..>5486)
                     /locus_tag="OO568_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6642936.1"
                     /translation="EISNKPYLGNMLNDFAGVDQQRVMHCRKAFSDAKVFK"
BASE COUNT         1564 a         1394 c         1217 g         1311 t
ORIGIN      
        1 tattgctaac cgcaatggtc aattgcagtt gaacgtgaca gatcgtaaaa ccggacaaac
       61 ctcgaccatc caggtttcgg gtttacaaaa taactcaacc gatttttaag ccccagcttc
      121 ataaggaaaa taatcatgca gcgcttattt cttttggttg ccgtcatgtt actgagcgga
      181 tgcttaaccg ccccgcctaa agaagccgcc agaccgacat taatgcctcg tgctcagagc
      241 tacaaagatt tgacccatct gccagcgccg acgggtaaga tctttgtttc ggtatacaac
      301 attcaggacg aaaccgggca atttaaaccc tacccggcaa gtaacttctc cactgctgtt
      361 ccgcaaagcg ccagggctta caaaacctgc ttaacgagcg caaggttatt cgtgcggcac
      421 aagaaaacgg cacggttgcc attaataacc gaatcccgct gcaatcttta acggcggcaa
      481 atattatggt tgaaggttcg attattggtt atgaaagcaa cgttaaatcg ggcggtgttg
      541 gggcaagata ttttgggatc ggtgcagaca cgcaatacca gctcgatcag attgccgtga
      601 acctgcgtgt cgtcaatgtg agtaccggtg agatcctctc ctcggtgaac accagtaaga
      661 cgatactttc ctatgaagtt caggccgggg ttttccgctt tattgactac cagcgcttgc
      721 tggaagggga agtgggttac acctcaaacg aacccgttat gctgtgtctg atgtcggcta
      781 tcgaaacagg ggtcattttc ctgattaatg atggtatcga ccgtggtctg tgggatttgc
      841 aaaataaagc agaacggcag aatgacattc tggtgaaata ccgccatatg tcggttccac
      901 cggaatcctg acagataaaa atgccggaat taataatccg gcatttactg tttaagcgaa
      961 agcgtgagtg cccctcacgc tttatttttg atttcctgct cacggtgagc cgtctttgca
     1021 ctacgcgccg ccagccacag gccgctgttt ttcattgcgt aaccaaatac cagcccaagc
     1081 gccagcgaag gtaacaccag tttccagtca ccctgcccgg caaacgtcgc gcatgcgcct
     1141 ataaacgtcc ccggaacaaa tgaaagcagc agctgtttgg cctgaataca catcagaaac
     1201 gcgacaatac cggttatgac ataaccgaga atttccagat gtggtgccag tgcactaccg
     1261 taaataatga ccatcgccca caccacgcca ctaagcaggg ttgcagcgga gatcgccagc
     1321 cctttcagcc caccttgcgg gcaagcaaag taggccgtac agcccaggaa gcccgcccag
     1381 cttagtaagc ccagggaaac agccacccat ccccagatac cggagagaat gcctgttgtg
     1441 attgcaatag agagaagtat gttcatgccg cgcatgatag cagaaaacgc ggccatgaat
     1501 gagatcgagc acacatttta tgcaacaaca aattatctgt tgcagttatg agtgacgatg
     1561 atcacattta ctcttcagaa tcttcttcca gctcgtccca cattgcacta atggcatcac
     1621 gggttagcgg tgccatggtg cgccagaaag gcgtggttgc atgggcctcc actttgccga
     1681 ggaacgcacc acaccagggt aacagatact cactgaacag tgtttccagt gcttcgctct
     1741 catcttccgt tgactgatct tccagccagg aagccgcaag cagcaatgtg ccgatgtgat
     1801 cggctggcgt atccgccagt ggcatccctc gttcggaaag aaaagcgcgc acttccgctt
     1861 ccgttgcgtc ctcaacccat gcgctacgat atggcggcac ggcacattca tcgccgataa
     1921 acaacgcatt gtaatcggca gagacttgcg tcatatcaca gcttttctgt aaacgcgtca
     1981 gtaactcatc ctgctccagt ggccagttcg cagccagttt cccctcacga atcagagtaa
     2041 acagcggcac cagtaaagga tcttgcggtt ggcggtaata gagcgaaccc agcacacgac
     2101 agaggataga aaactcgttc attaatatat ttccattact agtaattaaa ggtctgcaaa
     2161 ttccgcaatc ggtgccatac cgcgagattc aaggaagttc agtaagcggc gcggagaaac
     2221 attcaaaatg cgctctggcg gaaaatctac cgcgtcgagg attttaagac actcttcaaa
     2281 ttcccccatg gtaaacgcgg tgtgagaatc cgagcctaat gccacccaac ctccagcatc
     2341 acgtaccgct gcagctaccg cacgacagtt gtcttcactg cccttacgtg agtgtaaaaa
     2401 tgaggaatta ttgatttcca gcgccacctg atgtttcgcg gctgcttcag caacggcttt
     2461 aacatctatt tcatatttcg gatttccggg atggcttatt atatgcacat tgccgctggc
     2521 gatagtggcg atcatcgctt gtgtattggt cgctttgtca tgtggcgcaa aaaccggctc
     2581 atgaaaaccg gcaataatca gatccagcga gtcaaacatt ttaccgctgc agtcaatttc
     2641 accatcaaca tttttaatgt tagcttcaat gccgcgcagg atccctaccc catcaaccac
     2701 tcgcggccag atacgcatgt taatgaagtg ccagtgatgc ggcgcatctt ccatatccgg
     2761 gccatgatcg gtgatcgcaa aaagtttaat gcccttttgt ttggcctggg caatgtaatc
     2821 actcaatgtg ctatatgcat gtgtgctggc aacggtatgc atatgaaggt cgacgggata
     2881 cataactctc tcctgtattc aattcccgcc aaggatagca ggaatcctga tgctttatta
     2941 gtagccgcgt gcgcggtcga cttgcccgca gaccctctcc cctttttcga gctgggcaat
     3001 agtgcgagaa atgtactcca cagcttcagc gggacgggta atcgcggcga catgtggtgt
     3061 tatcgtcacg cgtggatgtt gccagagcgg actttcaggc ggtaagggtt cacgattaaa
     3121 aacatccagc attgcgcctt taactttccc gctatccagc gccgcgagca ggtcatcttc
     3181 cacaacatga acaccacgcg ccaggttgag gagatacgcg ccatcaggta atttttcgag
     3241 taattgttga ttaataatgc cgacggtttc aggggtattc ggtaacaaat taatcaatac
     3301 ccgacattgg ctcagaaatg cagacagttc ttcccgtccg gcaaagcttt gcacgccagg
     3361 ccacgattta cgggttcgac tccagcaacg cagcggaaag cgctaggttt gcagactctg
     3421 agcaacttta cagcccagta cgcctgcgcc caaaatgccg atggtaaaat cttcccgatg
     3481 atattcaggc agcggttgcc aatgcgaact attttgctgg atgcgataat catcaaaacg
     3541 tcgaaaccaa tgcagcacct gactgacagc atattcctgc atttgctcgc ccataccggt
     3601 atcttccagg cgaaaaagtg gaacagaagg gttcagcatt tcagggtgtg cctgtagctt
     3661 gctcaaaata gaatcaacac cggccccgag tgcgaacacc gctttaagat cgcgccctgc
     3721 cagcatttca acaggaggat gccagactaa aacataatca gcagagtcat tatctccgct
     3781 tttccatgcc ctgactcttg cctgaggaat agctttgcgc agtgcctcaa tccaccattg
     3841 ggtatcgaac gttgggtgat aaaagatgat atccatactg actcccgaaa agcgttgtgc
     3901 gaaatttatt cgcacttatc gttatgatct acaaaggcca ccagcataac aaatccgtgg
     3961 tcggtggcaa aaaaagcaga tttcgcttat taaaaccaca cattgattga agtttgaata
     4021 aacgcgcgat tctttcaaaa agtttgttga cctcaggtca tgatttccct aaattagcgc
     4081 ccgttccagc aagacaggaa cgacaatttg gtgaggtgtc cgagtggctg aaggagcacg
     4141 cctggaaagt gtgtatacgg caacgtatcg ggggttcgaa tccccccctc accgccatat
     4201 ttaaagaaga gcgcgtacga aagtacgggc ttttttttcg tatattgtac acaccgggtg
     4261 cccccgccca cgtctgttag ggcgagggga aattgtgcac atcaactgtc tggttactca
     4321 gtcaacaatc aaaatgcgca agatagtaca gaacatatat gcgttatctg gtcacttcct
     4381 gtaacttcct cacttattaa aatgccccca acggaaatac atgtcacatg ccatcataaa
     4441 atcgttatgc cagaggaatc gacaatggaa aaaatagtaa attaagcacg gtacatagca
     4501 gagtaaaaaa taggcttcaa accaaatccg ccattccaca agcgttgcca ataccgacac
     4561 cagcgtaatc cacatcattt caataaccag caatatgaat aaccgaagcc aaagcggctt
     4621 ggcaaaaaag gtcgacaacg tattttcact ggagaaaaac agatcgattt cctgcaaaaa
     4681 aaacctgccc agaaaagcgt accgcttgga gaagcgcaga aaaaacagat taatcgccaa
     4741 cactacgcca caaagaatga gcaatgcaat tatcgattta aacgttaacc actgcccctc
     4801 tgtcacaaaa gaatcactgt tacgcaacgg gctgatagta agcaggttaa caaccgttga
     4861 gatgcacacc gaaagcagca catagcgagt cagactttta tattttactt ttttcgctac
     4921 tgaaatagcg acctgcttga cataactggt gcggatcatc cgaaacagca gaaagatgac
     4981 caacggccca agtaacgaaa tcaggaacag cgcatataac gagggtagaa gctggtatag
     5041 cccccaactc attgcactca acatgattaa cgacagaaat cctgccacca gaggttgtgt
     5101 aagaaataat tctattttgt ggttctgctt tttcttaaac cacaaaaaat cgatattctt
     5161 cgtgacgcat ttataagccc agtacgcctt gagatcaaaa ataaaattat acagcaccag
     5221 ggccagaatc ccactaacta caccaatatc ctctgatgga aaagtaaacc ctgcattttt
     5281 ccaataaaag cctatcagaa taagtccaat agcataaatg tatatcagat ttcgtttatc
     5341 ctgaaagcga ataaaagtta aataatcagg aatcatttga atacctttgc atcactaaat
     5401 gctttacgac aatgcataac tcgttgctga tccacgccag caaaatcatt taacatattc
     5461 cccaaatagg gcttattact tatttc
//
LOCUS       DAJTYG010000170         5407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTYG010000170 DAJTYG010000000
VERSION     DAJTYG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.171"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(80..3388)
                     /gene="icsA"
                     /locus_tag="OO568_003799"
                     /gene_synonym="virG"
     CDS             complement(80..3388)
                     /gene="icsA"
                     /locus_tag="OO568_003799"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR6642937.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            3917..5119
                     /gene="virA"
                     /locus_tag="OO568_003800"
     CDS             3917..5119
                     /gene="virA"
                     /locus_tag="OO568_003800"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR6642938.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMSEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1686 a         1212 c          899 g         1610 t
ORIGIN      
        1 gtgcacaaaa acccaccgag cttttacacg gtgggtccca gagaaatgca ggacatcaac
       61 acgccctgca tttttattat cagaaggtat atttcacacc caaaatacct tgggtatctc
      121 tgtaactgtt attacccaat tgctgagata cgtttcccca gatgcttagg ttatctgcaa
      181 gacgcccccc cacaccagtt ttgagttcaa tcacattacg gttgcctatc tggtgatttg
      241 acatgccatt cataattaca ccatattgct ttgagctcca tttccaattc acttccaaaa
      301 atggttcgaa cttacgaaga gtctccttat cgatacaact ctgactaatt aaggatgtac
      361 gcaatcccaa ttttgcttga atattattga ccccagagga tgtgaccata gtgccattgt
      421 gttcaatata gtcgtttgct tttaccccat gcctagttat ctggacttgt ggattgagat
      481 acaatgcgtt gtcaatatta ttatgagcag tccagcgtat ggttgggtag atatagccag
      541 cttccaaagc tcctgttatt cctttgctgg aatatttctc accagttaac ccatctcctt
      601 tgactgtatt attaaaccaa ctgtactgca accaagcatc catatataga cctgttcgtt
      661 ctttttcatc ctgaaaccaa gaactgtaca aaccggcagt ataaccgctc acagttcctc
      721 gtgaactata actagtatac ttattatgag ttttgctatc ctgatatcca taagctccca
      781 taatacccac atgcaagcca tcatgatcag tgaagtttgt cttcaaaata tctccaccaa
      841 tctgataaac catcctgtta gctgtggttt ttaattgtct atcagctaaa ttatgctcat
      901 tatgtccacc aacagtacga atccacatgg tggtttcgct gagctgacct gtagtaggat
      961 caacaatagt gctaccgtgt cggtcataga gagaaggact aaacatcgtg ttcattgcta
     1021 tcaggttagc aatataactg ccagcttcag ggcgccatgt gtgaatacta ccttcaccca
     1081 aattaagtga agatacaagc tgtgaactag ctcggcgact actcatttga gtagactctt
     1141 gattgctcat ctgttttgat tcttgattat ccatctgact agttagatac cacttattgg
     1201 tattcagtcc agatacagtg ccctgtttca ggcgatagtc ataactacca gcaacaatac
     1261 ggcctttctg aataaaagca ttcttatcag aagaatctgt cgaaataatt tgaacacctt
     1321 caagagtttt atctccagag cctccctcat ttgtcactgc aacataggta ataccagacg
     1381 catggcctgc aatttccagc cggtcagttt tactgttgtc attgccgaga accgcgccca
     1441 aagataatat tccgcccttt ccatgccaat tacctttctg aacataggtc tgaccgacat
     1501 ttgacgaact attattgaga ataacatgac cgtttttctc cagagtcatg ttacctgtga
     1561 caatgacggg gtcaggcaaa aaaggggcat taatattatt gattagcaca gtcccactat
     1621 ttaataaagt gttaacagtt tggttcatgc ctgaaaaatt caaggtagca cctttattaa
     1681 caataacacc agcggtacgt gtcatagctt caactgtccc catttttaga atgcctgcat
     1741 tgatgttggt gaaaccagag taggtatttt tttccgccaa aatgagagtt ccctcccctg
     1801 attttgttag ggattttccg tcccagctat ttatattatg atggctgaga ttatctgcca
     1861 gaatagtact aacagtaagt tcagcgtttt ctttcagatt gaactctcca tgactgtcgc
     1921 catctgtatc attccagctc aaaccgtaga tcaatttatt ctggtcactg atatagcttt
     1981 ttgtatagtc aatagcacca gtagtgagta acggattgat gatagatata ttagaaacac
     2041 tgaaacctgt tatcccttta tctgcactta gcagtttata gtccttctgc gcccagttat
     2101 ttataatagc ttccactttt acattgttgc caaatgacat atttccagaa gcttccagac
     2161 tactgattcc agctaaattt aaagaactgt tctcaccaaa agaaacgtca cctgaaacag
     2221 tgaatttgtt acctgatagt gaaacagttg tacaatcacc agcacataaa tcaccttcaa
     2281 tagttggtgt ggtcccagtg taatcattgt tgataatttt cagaatggat gaattgttaa
     2341 cctgtacatc accagtaatg acagaacctt catggagtat taatgaattg tttccaccag
     2401 tgatgtggat agcattacct gctacatttt gtgttccatc atcttcttta cctgaaatta
     2461 tatatccgct attagcaatg gtcatattgc taccatttat agcatccccc caatgtccac
     2521 cattaccgcc taaaatagct ccattgttga ttacagacag gttatctcct gtgatagcat
     2581 taccaccgta gccatcataa ccataaccgt aagcagcacc tccgttgcct ccagcgaacg
     2641 tgcccttatt gattacagag aggctgcttc ctgtgatggc atcacctcca ttaccgccat
     2701 caccctcacc ataattgtta cctccattac caccggaaat agtccctttg ttgattatgg
     2761 gtagattgac tcctgtgacg gcatcacctc cattcccccc atcactatca ctataactat
     2821 cgccaccatg tccacctgaa ataatttctc catttattat aaacagattg tctcctgtaa
     2881 cagcctcacc accatcaccg ttatggtcag caccgctacc gccgctacca ccaagaatca
     2941 tgccttgatt gattatagac aagtcagatc cggtaataga gtcaccacca ttaccgccac
     3001 aactattacc atcattatta tcaccagcac caccatgacc tgcgctaatc ataatatcag
     3061 agttaatccg tagcttagtt ctagatgcat gagaggggtt ggaagaagtt atataccacg
     3121 catccattcc atcttcacca gctcccaagg gagaaagtcc atcaacaggt gttaataatt
     3181 tttcataatt gtcctctgaa aaatgaagtt cttgagtacc cgaaagagga gtagcaaaag
     3241 ctattggccc cccgagcaac agggatgcac caacaacaaa aggagaaaaa gacaatttgc
     3301 atgttttttc ctttaccgta ggtaattctc cggccccccc ctgtgaacat tgggtcatat
     3361 tacaaaaaaa tttgtgaatt tgattcatgc actatattat cagtaagtgg ttgataaacc
     3421 cctgaaaaga ttccgtttta tccggaataa agggacgata tatgcaaaaa catattaaac
     3481 aaagcctcaa ccaaaacatg agagctgtgt tatatatcaa tgcaacggga gaaatttacc
     3541 tgtgtcttgc tttgaaaaaa agagggggaa agctatatgt tcgataataa attaataatt
     3601 ttactatgat agtctgcttc agaccgcaac ttttcagtcc tttctgtacg cttgcccaaa
     3661 gtttaaccac tttgtttttc tatacaccca atggttctat ctgccccttg atgttaatcc
     3721 ccaaagccaa taacatgaat gtattcatat cacaactttc ctaccagact ctccaatgca
     3781 ataactgcat aattgttttt agtggaaatg tatcgttttc ttaaagagaa gaataacatt
     3841 ccattttatt atgttcccaa tttaattata ttaatatata ttggcattaa taggaaaata
     3901 catcaggaga aatcaaatgc agacatcaaa cataactaac catgaaagaa atgactcatc
     3961 atggatgtca actgtcaaat caactacaga agtaagttgg aataaattga gtttttgtga
     4021 cgttttactg aaaataataa ctttcggcat atattctcca cacgaaactc tagccgaaaa
     4081 atatagcgaa aaaaaactaa tggatagttt ctcaccatca ctatcacaag ataaaatgga
     4141 cggagagttt gcccacgcaa acatagatgg catttcaata cggctatgcc ttaacaaagg
     4201 catatgttcg gttttttatc ttgatggaga taaaatacaa tccactcaac tttcatcaaa
     4261 agaatataat aacttgttaa gttcactccc ccccaaacaa ttcaacttag gaaaagtaca
     4321 tactattaca gctccggtat cagggaattt taaaactcac aagcctgcac cagaagttat
     4381 tgaaacagcc attaattgct gtacatcgat aataccaaac gatgattact tccacgttaa
     4441 agacactgat tttaatagtg tttggcatga tatttacaga gatattcgag catctgacag
     4501 caatagtaca aaaatatatt tcaacaatat tgaaatacca ctaaaattaa ttgcagatct
     4561 aattaatgag ttaggaatta atgaattcat tgatagcaaa aaagagcttc aaatgctaag
     4621 ttacaaccaa gtaaataaaa taatcaattc aaacttcccc caacaagacc tatgttttca
     4681 aacagaaaaa cttttgttta cttcactctt tcaagaccct gcatttatat ctgcactaac
     4741 atctgcattc tggcaatctc ttcacatcac gtcttcctct gtggaacaca tatatgccca
     4801 aattatgtcc gaaaacatag aaaacaggct taattttatg tctgaacaac gagttattaa
     4861 caattgtgga catattataa aaatcaacgc agttgtccct aaaaacgaca ctgcaatctc
     4921 tgcctctggg gggagggctt acgaagttag ctcatcaata ttaccatcgc acataacatg
     4981 taatggtgtt ggtattaata aaatagaaac aagctatctc gttcatgctg gaacacttcc
     5041 aagcagtgaa gggcttcgca atgctatccc tccagaaagt cgtcaagtat catttgccat
     5101 catatctcct gatgtttaag ccaatattat attggctgcc agactgtaaa aaaacgttct
     5161 tcagaacagc tctataaact attaaaccaa agctaaaagg atggggacca atagaatctc
     5221 agcccatcct ttttatatca ttatgaaccg ccccggaaat cctggagact aaactccctg
     5281 agaaagaggt aaacaggatg actaaaaata cacgtttttc ccctgaagtc cgtcaacggg
     5341 cagttcgtat ggttctggaa agtcagggcg aatataactc acaatgggca gcgatttgtt
     5401 ccattgc
//
LOCUS       DAJTYG010000171         5405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTYG010000171 DAJTYG010000000
VERSION     DAJTYG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.172"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            244..1068
                     /gene="gadX"
                     /locus_tag="OO568_003801"
     CDS             244..1068
                     /gene="gadX"
                     /locus_tag="OO568_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6642939.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1436..2164
                     /gene="gadW"
                     /locus_tag="OO568_003802"
     CDS             1436..2164
                     /gene="gadW"
                     /locus_tag="OO568_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6642940.1"
                     /translation="MTYVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2527..>5405)
                     /gene="mdtF"
                     /locus_tag="OO568_003803"
     CDS             complement(2527..>5405)
                     /gene="mdtF"
                     /locus_tag="OO568_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HCR6642941.1"
                     /translation="STSDAAGNASITLTFETGTSPDIAQVQVQNKLQLAMPSLPEAVQ
                     QQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVASNIKDPLSRTAGVGSVQLFGSE
                     YAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQLGGMPQVADQQLNASIIVQTR
                     LQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYSTVARYNGKPAAGIAIKLAAGA
                     NALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIEISIQEVFKTLVEAIILVFLVM
                     YLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINTLTMFGMVLAIGLLVDDAIVVV
                     ENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLSAVFMPMAFMSGATGEIYRQFS
                     ITLISSMRLSVFVAMSLTPALCATILKAAPEGGHKPNALFARFNTLFEKSTQHYTDST
                     RSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEEDQGVFMTTAQLPSGATMVNTTK
                     VLQQVTDYYLTKEKDNVQSVFTVGSFGFSGQGQNNGLAFISLKPWSERVGEENSVTAI
                     IQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMELLDNGNLGHEKLTQARNELLSL
                     AAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVALSDINQTISTAFGISYVNDFL
                     NQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAPLSAYSSTEWTYGSPRLERYNG
                     IPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYSWTGLSYQEALSSNQAPALYAI
                     SLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLATDLRGLSNDVYFQVGLLTTIGL
                     SAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRPILMTSLAFILGVLPLVISHGA
                     GSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHLFARFKKA"
BASE COUNT         1418 a         1265 c         1279 g         1443 t
ORIGIN      
        1 tgtttattaa atgtagcgat tacctttaat gtcacgaaaa gggattattt gcttgctatt
       61 aatttcactg cgttcttaaa cacgtttttg tattataaat ccagtcatcc tgcccgtaga
      121 acatcacaca ttatcatcct gttctcccgc ttcgtttaaa tttatttatc aatcaatttg
      181 acttaagagg gcggcgtgct acattaatta acagtaatat gtttatgtaa tattaagtca
      241 actatgcaat cattacatgg gaattgtcta attgcgtacg caagacataa atatattctc
      301 accatggtta atggtgaata tcgctatttt aatggcggtg acctggtttt tgcggatgca
      361 agccaaattc gagtagataa gtgtgttgaa aattttgtat tagtatcaag ggatacgctt
      421 tcattatttc tgccgatgct caaggaggag gcattaaatc ttcatgcaca taaaaaaatt
      481 tcttcattac tcgttcatca ctgtagcaga gatattcctg tttttcagga agttgcgcaa
      541 ctatcgcaga ataagaatct tcgctatgca gaaatgctac gtaaaagagc attaatcttt
      601 gcgttgttat ctgtttttct tgaggatgag cactttatac cgctgctttt gaacgttttg
      661 caaccgaaca tgcgaacacg agtttgtacg gttatcaata ataatatcgc ccatgagtgg
      721 acactagccc gaatcgccag cgagctgttg atgagtccaa gcctgttaaa gaaaaaattg
      781 cgcgaagaag agacatcata ttcacagttg cttactgagt gtagaatgca acgtgctttg
      841 caacttattg ttatacatgg cttttcaatt aagcgagtcg cagtgtcctg tggatatcac
      901 agcgtgtcgt atttcattta cgtctttcga aattattatg ggatgacgcc cacagagtat
      961 caggagcgat cggcgcaggg attgccgaac cgtgactcgg cggcaagtat tgttgcgcaa
     1021 gggaattttt acggcactaa ccgttctgcg gaaggaataa gattatagag ttttactcag
     1081 acataaaaaa aacccggcat aggggaccgg gaagaggata gtctgccgtc tccagactaa
     1141 taaaccgtta taacactccc tgttggcacg ggaaactttg tgctctcagt aagttaaata
     1201 taacttttac tggaaataag atcagccatt tttttataaa cataagctat acgctgtgcg
     1261 aaaatatatt cgtgctgcat ttacttatta tcaattaact gttatgcaaa actactttgt
     1321 ggataaattt tggtcctacc aaatctggca gtttttgcgc taagaaacag tctgtcatca
     1381 tttcattagt atactgaaat tgaaataatc gcagtatgaa atataaggga taatcatgac
     1441 ttatgtctgc tcggtgatcc tcattcgtcg ttcattcgat atttatcatg aacagcaaaa
     1501 aatatcgctg cataacgaga gtatcctgct gctggagaaa aatttggcag acgattttgc
     1561 gttttgttca ccggatacgc gacgactgga tatcgatgag ctgacagttt gccattactt
     1621 acaaaatatt cgtcagctac cacgcaattt agggttacat agcaaagacc gtctgttaat
     1681 taaccagtca ccccccatgc cgctggtgac ggcgattttt gatagcttca atgaatccgg
     1741 ggtaaattca ccgatactga gcaatatgct ctacctttcc tgtttatcga tgttttctca
     1801 taagaaagaa ctgatcccct tacttttcaa tagcatcagc actgtttcag gaaaagttga
     1861 acgccttatt agctttgata tcgccaaacg ttggtatctg cgcgatatcg cggaaagaat
     1921 gtataccagc gagagtctca tcaaaaaaaa gttgcaggat gaaaatacct gtttcagtaa
     1981 aatattactc gcttccagga tgtcgatggc cagacgatta ctcgagttac gtcaaattcc
     2041 tctgcatact attgcggaaa aatgtggcta tagcagtacg tcgtacttta taaacacatt
     2101 tcgacaatat tatggtgtaa cgccacatca gtttgcgcaa cattcgccag gtaccttttc
     2161 ctgacatatt ttgcatttga atattggtca ggatctcaca cctgcttcat gtgaaactct
     2221 tccctgatga tttctgccgg gctaccggct agttctcttt cgcagtttgc caggctgtat
     2281 aggtattttc aggaggaaaa gactcggcat gtttgggatt attaagctga caattcatac
     2341 gattacggga atgtgggtca gtattgtgtt gttcaaattg atgacgaatg gctggagtgg
     2401 tttctacttt cagtgctgtg tactaagcct ggtattcctg acggttagct ggttgttgag
     2461 tggtgaatgg ttagcaggaa agagtaaggc tgaaccttca cgttcaacct tactctcatt
     2521 tacacgttac gcttttttaa agcgggcaaa gagatgttcc actacaacga aaaagaccgg
     2581 aacgaagtaa attgccagca ctgttgcggc aaacatcccg cccatcacgc cggtacctac
     2641 cgcgttttgc gcgccagaac cggcaccatg actgataacc agcggcagca cgccgagaat
     2701 aaaggccaga gaggtcatca ggattgggcg taaacgcatc cgcgccgctt cgatgattgc
     2761 ctctatcggc gttttccctt ctttctgcat catctcaacg gcaaattcga caatcaggat
     2821 ggcgttcttg gcggaaagtc cgatggtggt cagcaaacca acctggaagt agacgtcatt
     2881 acttaagccg cgcagatcgg tggccagtaa tgcgccaacg acgcctaacg gaacaaccaa
     2941 catcaccgag aacggaattg accaactctc atagagtgcg gcgagggcga ggaacaccac
     3001 gaccagtgaa atggcataca gtgcaggagc ctggtttgag gataacgctt cctgatacga
     3061 cagtccggtc catgagtagc cgacgcctgc cggaagttta gcgaccaggt ctgccataaa
     3121 tttcatagcg tcaccggtac ttttcccggc cgccgcttca cctaaaatct ccattgacgg
     3181 gatgccgttg tagcgttcca atcgcggtga accataggtc cattcggtag acgagtaggc
     3241 agaaagcggt gccatcgtgc cagaggcgtt gcgtacatac cattggttga tgttatccgg
     3301 caacatacgg aacggcgtgc ctgcctggac atacactttt ttcacccgcc cctggttgag
     3361 gaagtcgttc acgtagctga tgccgaaggc ggtggaaatt gtctggttga tatcagacag
     3421 cgccacaccc attgcctcag ctttcgcggc gttgacgttc actttgaaca tcggcgtatc
     3481 ttccaggccg ttcgggcgta ccccggtgac ctggtcaggc gattgcgctg ccagtgataa
     3541 cagctcgttt cgtgcctggg ttagtttttc gtgccccagg ttaccgttgt ccagcagttc
     3601 catatcaaaa cctgacgcgg tacccagttc agccaccgcg ggtaagttga acgggaagac
     3661 gacggcttta ttgatactgc ttaacgcaat cattgcccgc tgaatgatcg cggtaaccga
     3721 gttttcctca ccgacacgtt cagaccacgg cttgagactg ataaacgcca ggccgttgtt
     3781 ttgcccctgg ccgctgaagc caaagctgcc aacggtaaac accgactgaa cattatcttt
     3841 ctcttttgta agataataat ccgtcacctg ttgcagtact ttcgtggtgt taaccatggt
     3901 ggcaccggaa ggtaactgcg cggtggtcat aaataccccc tgatcctctt ctggtaagaa
     3961 agaggtcggt gtacgcagga acagcaccgc catcccggcg caaatcagca ggtagaccac
     4021 catgtagcga ccggtacaac gcaacagcga gcgggtgcta tcggtatagt gctgagttga
     4081 tttttcaaac agcgtgttga agcgtgcgaa cagggcgtta ggtttgtgac cgccttccgg
     4141 cgcggctttc agaatggtgg cgcacagggc aggggtcagg ctcattgcca caaatactga
     4201 aagccgcatg gaggagatca gcgtgatgga gaactggcgg tagatctccc cggttgcacc
     4261 gctcataaag gccatcggca taaacactgc agaaagaaca acggcaatcc cgaccagcgc
     4321 acgttggatc tgccccatcg atttatgcgt cgcttccttc ggcggtagct tatcttccgc
     4381 aatgacacgc tcgacgttct ccaccaccac gatggcgtca tccaccagta accctatcgc
     4441 cagcaccatc ccgaacatag tcaacgtgtt gatggtgaaa ccgaccgccg acaagatcgc
     4501 aaacgtcccg agaataacca ccggtacggc aattgtcggg atgatggtgg cacggaaatt
     4561 ctgcaaaaac agatacatga ccaggaagac gaggatgata gcctcaacca gtgttttgaa
     4621 aacttcctga atagaaattt cgataaacgg cgtggtgtcg taaggataaa ccgtcttcag
     4681 acttgcgggg aaataggctg ataagcggtt cagctcctct ttgactgccc gcgaggtatc
     4741 cagggcgttt gctccggcag ccagtttgat ggcgatcccg gcagcaggtt tgccgttata
     4801 gcgcgccacg gtggaataat cttccgcccc aagttcgacg cgggcgacat cacgcagcag
     4861 cacttgcgaa ccatcttgct gaactttcaa caggattttg ccaaattctt ccggcgtttg
     4921 cagacgcgtc tgcacaatga tcgaggcgtt tagttgctgg tctgccacct gtggcatgcc
     4981 acccagttga ccaccagaaa tctggttgtt ttgcacctta atctgggaaa taacatcgga
     5041 aggtaccagg ttatatttat tgagtttttg cgggtccagc cagatacgca tagcgtactc
     5101 ggaaccaaag agctgtacgc taccaacgcc cgcggtacgg cttagcgggt cttttatatt
     5161 agacgctaca tagtccgcga tatcgtactg gttgaggctg ccgttatcag aaataaacgc
     5221 cgctaccatc aggatattac tgctcgactt atcgacgcta atcccctgtt gctgcaccgc
     5281 ttcaggtaat gaaggcatag cgagttgcag tttattttgc acctgaacct gtgcgatatc
     5341 aggagatgtc ccagtctcga aggtcagagt gatagaggca ttacccgccg catcactggt
     5401 tgaag
//
LOCUS       DAJTYG010000172         5337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTYG010000172 DAJTYG010000000
VERSION     DAJTYG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.173"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO568_003804"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO568_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6642942.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OO568_003805"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OO568_003805"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6642943.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINSEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNSLLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OO568_003806"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OO568_003806"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6642944.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OO568_003807"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OO568_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6642945.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEINEDRIVAV
                     Q"
BASE COUNT         1309 a         1289 c         1455 g         1284 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaactc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaattcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggt
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaaattaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DAJTYG010000173         5315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTYG010000173 DAJTYG010000000
VERSION     DAJTYG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.174"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..746
                     /locus_tag="OO568_003808"
     CDS             <1..746
                     /locus_tag="OO568_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6642946.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="OO568_003809"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="OO568_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6642947.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3804
                     /gene="safA"
                     /locus_tag="OO568_003810"
     CDS             3607..3804
                     /gene="safA"
                     /locus_tag="OO568_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6642948.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="OO568_003811"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="OO568_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6642949.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTEPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5045..>5315
                     /locus_tag="OO568_003812"
     CDS             5045..>5315
                     /locus_tag="OO568_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6642950.1"
                     /translation="MSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAFS
                     DFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVV"
BASE COUNT         1531 a         1131 c         1178 g         1475 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgaa aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ctactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagcacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ctgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg cattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tactcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaaca ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg caattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctgaaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctctgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gagcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 aatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttgtttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atgaatgtct gcattaaaga aaagtgtcgt aagtacctcg atatctttga tactggcatc
     5101 tggtatggct gcatttgctg ctcatgcggc agatgatgta aagctgaaag caaccaaaac
     5161 aaacgttgct ttctcagact ttacgccgac tgaatacagt accaaaggaa agccaaatat
     5221 tatcgtacta accatggatg atcttggtta tggacaactt ccttttgata agggatcttt
     5281 tgacccaaaa acaatggaaa atcgtgaagt tgtcg
//
LOCUS       DAJTYG010000174         5295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTYG010000174 DAJTYG010000000
VERSION     DAJTYG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.175"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..584
                     /locus_tag="OO568_003813"
                     /pseudo
     CDS             1..584
                     /locus_tag="OO568_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            648..1574
                     /gene="glsB"
                     /locus_tag="OO568_003814"
     CDS             648..1574
                     /gene="glsB"
                     /locus_tag="OO568_003814"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6642951.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEVAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            1574..1933
                     /locus_tag="OO568_003815"
     CDS             1574..1933
                     /locus_tag="OO568_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR6642952.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQPPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            2072..3490
                     /locus_tag="OO568_003816"
     CDS             2072..3490
                     /locus_tag="OO568_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6642953.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIELIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            3717..5168
                     /gene="uxaB"
                     /locus_tag="OO568_003817"
     CDS             3717..5168
                     /gene="uxaB"
                     /locus_tag="OO568_003817"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6642954.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1225 a         1233 c         1423 g         1414 t
ORIGIN      
        1 tcccatcatt cttgcaggta acggctactt acttaaacat gcgccgaatg tgatgggctg
       61 tgctcagctc attgcccagg tgtttaaaga tgcgggaatc ccgcaaggcg tatatggctg
      121 gctgaatgcc gacaacgacg gtgtcagtca gatgattaaa gactcgcgca ttgctgctgt
      181 cacggtgacc ggaagtgttc gtgctggagc ggcactgaaa aaatgcgtac tactatccgc
      241 caacggttct ggcgaatgtt accccagaaa tgaccgcgtt tcgggaagaa atgtttggcc
      301 ccgttgctgc aatcaccatt gcgaaagatg cagaacatgc gctggaactg gctaatgata
      361 gtgagttcgg cctttcagcg accattttta ccaccgacga aacacaggcc agacagatgg
      421 cggcacgtct ggaatgcggt ggggtgttta tcaatggtta ttgtgccagc gacgcgcgag
      481 tggcctttgg tggcgtgaaa aagagtggct ttggtcgtga gctttcccat ttcggcttac
      541 acgaattctg taatatccag acagtgtgga aagaccggat ctgacgcgac atattcagct
      601 ctgatatact cgcaggtctt ttcagacctg cggtccagga gtagaaagtg gcagtcgcca
      661 tggataatgc aattttagaa aacatcttgc ggcaagtgcg gccgctcatt ggtcagggta
      721 aagtcgcgga ttatattccg gcgctggcta cagtagacgg ttcccgattg gggattgcta
      781 tctgtaccgt tgacggacag ctttttcagg ccggagacgc gcaagaacgt ttttccattc
      841 agtctatttc caaagtgctg agtctcgttg tcgccatgcg tcattactcc gaagaggaaa
      901 tctggcaacg cgtcggcaaa gatccgtctg gatcaccgtt caattcttta gtgcaactgg
      961 aaatggaaca gggtattccg cgtaatccgt tcattaatgc cggtgcgctg gtggtctgcg
     1021 atatgttgca agggcgacta agcgcaccac ggcaacgtat gctggaagtc gtgcgcggct
     1081 taagcggtgt gtctgatatt tcatacgata cggtggtagc gcgttccgaa tttgaacatt
     1141 ccgcgcgaaa tgcggctatc gcttggctga tgaagtcgtt tggcaatttc catcatgacg
     1201 tgacaaccgt tctgcaaaac tactttcatt actgcgctct gaaaatgagc tgtgtagagc
     1261 tggcccggac gtttgtcttt ctggctaatc aggggaaagc tattcatatt gatgaacctg
     1321 tggtgacgcc aatgcaggcg cggcaaatta acgcgctgat ggcgaccagt ggtatgtacc
     1381 agaacgcggg ggaggttgcc tggcgggtgg gactaccggc gaaatctggc gttggtggcg
     1441 gtattgtggc gattgttccg catgaaatgg ccatcgcagt ctggagtccg gaactggatg
     1501 atgcaggtaa ctcgcttgcg ggtatcgccg tccttgaaca attgacgaaa cagttagggc
     1561 gttcggttta ttaatgcagt ctctcgatcc actcttcgcg cgtttatccc gttcaaaatt
     1621 tcgctctcgc tttcgtctgg gcatgaaaga gcgtcagtat tgcctggaga aaggcgcgcc
     1681 agtcatcgaa caacatgcgg cggattttgt cgctaaacgt cttgctccgg cattaccggc
     1741 taatgacggt aagcaacccc ccatgcgcgg gcatccggtg tttatcgctc agcacgccac
     1801 cgcgacctgc tgtcgcggtt gtctggctaa gtggcataac attccgcagg gtgtttcgtt
     1861 aagcgaagaa caacaacgtt atatcgtggc ggtaatctat cactggctgg ttgtacagat
     1921 gaatcagcca tgagaattgc ggaaaacgat aaaatagcat tcccagggta cctttatttg
     1981 ccgccgctgt tagcgaacat gctatccttt atgagaattt acttatcacg ttgagataat
     2041 tgccacccgc aattgtatgg acagataatt aatgcatgta caccccattt caactttccg
     2101 tttgttccag gaaggtcatc tgctacgtaa tagcatcgct atttttgcgc taaccacgct
     2161 gttctatttt attggtgcag agttacggct ggttcacgaa ctttctcttt tctggccgct
     2221 gaatggcgta atggcggggg tgtttgcccg ctatgtctgg cttaatcgac tgcattacta
     2281 tgcgatcagt tatgtggcga tgctggttta tgatgccata accaccgaat ggggactggt
     2341 ttcactggtt atcaatttct ccaatatgat gtttattgtt accgtcgcct tactggtcgt
     2401 gcgggataag cgtctgggga aaaataagta tgagccagta agtgccttac ggctatttaa
     2461 ttactgtctg attgccgcat tattatgcgc tattgtcggg gcgattggtt cggtcagtat
     2521 tgatagtctg gatttctggc ctttgcttgc ggactggttc agtgagcaat tctcaacggg
     2581 cgtgttgatc gtgccttgta tgctgacgtt ggctattcct ggagtgctgc cgcgctttaa
     2641 agcagaacag atgatgcccg ccatcgagct tattgtgtcg gttattgcct cggtagtcat
     2701 tggcggagcg gggagtctgg cgttcccgct gcctgcatta atctggtgtg cagtgcgcta
     2761 tacgccgcag gtaacatgtc tgttgacctt tgtcaccggt gcggtggaaa tcgtactggt
     2821 ggcaaattcg gtgattgata tctcggtcgg ttcgccgttc tccattccgg aaatgttctc
     2881 cgcacgtctc ggtattgcca cgatggcgat atgcccaatt atggtttctt ttagcgtggc
     2941 agcgatcaat ttgctaatga agcaagttgc gctgcgagcc gactttgatt ttctgactca
     3001 ggtttactca cggtccggtc tttatgaggc gctgaaaagt ccatcgctga aacagacaca
     3061 acatctgacg gtcatgctgc ttgatatcga ctatttcaaa agcattaacg ataactatgg
     3121 acatgaatgt ggcgataaag tgttaagcgt gtttgcccgg catattcaga agattgtcgg
     3181 tgataagggg ctggtggcgc gcatgggcgg cgaagaattt gctgttgcag tgccgtcggt
     3241 gaatcctgtc gatggtctgc taatggcgga aaaaatccgt aaaggcgttg aactgcaacc
     3301 attcacctgg caacaaaaaa cgctttatct cacggtaagt attggcgtcg gtagtggtcg
     3361 cgcatcgtac cgaacgctga ccgatgattt taataaattg atggtcgaag ccgatacgtg
     3421 tctgtatcgc tcgaagaaag atgggcgcaa ccgtaccagc actatgcgtt acggtgagga
     3481 agttgtctga catctttatt cattccgcgc gatttgaatg caatgtgttc taccacttta
     3541 tgatccagga aaaggcgtat ttcctcgatt aaaccatgat ccgcgccaca cttttctcat
     3601 aaaactgctc attaatcatc aggagttgat caaaacaggg gttcggcgta ttaaagtttg
     3661 ctaacctgtc gcaccaattc aaacaggttg tatgactaat cagaagggaa cccattgtga
     3721 aaacactaaa tcgtcgcgat tttcccggtg cacagtatcc agaacgtatc attcagtttg
     3781 gtgaaggtaa cttcctgcgc gcctttgttg actggcaaat cgatctcctg aatgagcaca
     3841 ccgatctgaa ttctggcgtg gtcgttgttc gtccgattga aacttcattc ccgccgtcac
     3901 tgagcacgca ggatggtctg tacaccacca ttatccgtgg cctgaatgag aagggggaag
     3961 cggtcagcga cgcgcgtctg attcgctcgg ttaatcgtga aatcagcgtc tacagtgaat
     4021 acgatgaatt cctgaaactg gcgcacaacc cggaaatgcg ctttgttttc tctaacacca
     4081 ccgaagcggg tatcagctac catgcgggcg ataaattcga tgacgcgcca gcggtaagct
     4141 atccggcaaa actgacccgt ctgctgttcg aacgctttag ccatttcaac ggtgcgctgg
     4201 ataaaggttg gatcatcatt ccgtgtgagt tgattgacta caatggcgac gcgttgcgtg
     4261 aactggtgct gcgctatgcg caagagtggg cactgccaga agcatttatt caatggctgg
     4321 atcaggctaa cagcttctgt tctacgctgg tggaccgtat cgttaccggt tatccgcgcg
     4381 atgaagtggc taaactggaa gaagaactgg gttatcacga tggtttcctc gacaccgctg
     4441 aacactttta cctgtttgtg attcaggggc cgaaatcctt agcgactgag ctgcgcctgg
     4501 acaaatatcc gctcaacgtg ctgattgttg acgatattaa accgtataaa gaacgcaaag
     4561 tggcaatcct caacggtgcg cacaccgcgc tggtgccggt ggcgttccag gcagggctgg
     4621 ataccgtagg tgaagcgatg aacgatgctg aaatttgcgc attcgttgaa aaagccatct
     4681 acgaagaaat tattccggta ctggatttgc ctcgtgatga actggaatct ttcgccagtg
     4741 cagtcaccgg gcgtttccgt aacccgtaca ttaagcatca gctgctgtct atcgcgctca
     4801 acggtatgac caagttccgc acccgcatcc tgccgcagct gctggcaggg cagaaggcaa
     4861 acggcacact tccggcgcgc cttactttcg cattagcggc attgattgcg ttctatcgtg
     4921 gtgagcgtaa cggagaaaca tatccggtac aggacgatgc acactggctg gaacgttatc
     4981 agcaactctg gagccagcat cgtgaccgtg ttatcggtac ccaggaactg gtagcgattg
     5041 tgttggcaga aaaagaccac tgggagcagg acctgacgca agtgcctggt ctggtcgagc
     5101 aggttgctaa tgacctggat gcgattcttg aaaaagggat gcgcgaagct gtacgtccgt
     5161 tgtgctaata acaccgttac ttctgaaccc ggtttcgacc gggtttctta tttcactata
     5221 ttagttacaa catcctatat gcctttatca ttaacaggtg tcacatgcag cgcctgttaa
     5281 tgttgtaagc ttgac
//
LOCUS       DAJTYG010000175         5251 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTYG010000175 DAJTYG010000000
VERSION     DAJTYG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.176"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..446)
                     /locus_tag="OO568_003818"
     CDS             complement(<1..446)
                     /locus_tag="OO568_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR6642955.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /gene="manZ"
                     /locus_tag="OO568_003819"
     CDS             complement(501..1352)
                     /gene="manZ"
                     /locus_tag="OO568_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6642956.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALSAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /gene="manY"
                     /locus_tag="OO568_003820"
     CDS             complement(1365..2165)
                     /gene="manY"
                     /locus_tag="OO568_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6642957.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="OO568_003821"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="OO568_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6642958.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAANRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="OO568_003822"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="OO568_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6642959.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFFFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLIKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
BASE COUNT         1284 a         1318 c         1332 g         1317 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 atgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacaacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcact cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggcc ccattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcaac cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggttgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtattttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttcttcttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt atcgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tgataaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcctt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat t
//
LOCUS       DAJTYG010000176         5226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTYG010000176 DAJTYG010000000
VERSION     DAJTYG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.177"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..213
                     /locus_tag="OO568_003823"
     CDS             <1..213
                     /locus_tag="OO568_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6642960.1"
                     /translation="PQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEEL
                     SNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            356..577
                     /locus_tag="OO568_003824"
                     /pseudo
     CDS             356..577
                     /locus_tag="OO568_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(598..660)
                     /locus_tag="OO568_003825"
                     /pseudo
     CDS             complement(598..660)
                     /locus_tag="OO568_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1110..2522
                     /gene="ospC1"
                     /locus_tag="OO568_003826"
     CDS             1110..2522
                     /gene="ospC1"
                     /locus_tag="OO568_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR6642961.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHGSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2626..2775)
                     /locus_tag="OO568_003827"
                     /pseudo
     CDS             complement(2626..2775)
                     /locus_tag="OO568_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2851..4548
                     /gene="ospD3"
                     /locus_tag="OO568_003828"
     CDS             2851..4548
                     /gene="ospD3"
                     /locus_tag="OO568_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR6642962.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLNPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            4665..4784
                     /locus_tag="OO568_003829"
                     /pseudo
     CDS             4665..4784
                     /locus_tag="OO568_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
     gene            4951..5223
                     /locus_tag="OO568_003830"
     CDS             4951..5223
                     /locus_tag="OO568_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642963.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT         1764 a          985 c         1010 g         1467 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatggggggc
      121 ggtgaagaat tatctaacct tgtcttgtta catccaaatt gccatcgaca gttacacagt
      181 tgtgaagccg gctctctcaa aaagggctta taaaggcttg agcggtgtac cgggaaaccg
      241 gtatgcaccg ttcttagggg agggcagtcc agcaatgaac tgtccttacc cgacaaacat
      301 ccaaaacgaa cggaacgtgc tggaatccgc atataacccg ttgtaaagtg aggaaatgga
      361 cacctcactt gctcatgaga acgcccgcct gcgggcactg ttgcagacgc aacaggacac
      421 tatccaccag atggctgaat acaaccgcct gctctcacag cggatggcgg cttatgcttc
      481 cgaaatcaac cgactgaagg cgctggttgc gaaactgcag cgtatgcagt tcggtaaaag
      541 ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca ttttcacggg cgatctacgc
      601 cacacaggct ctgggttgca gtgactgctc cattaaggcg attttgaact cataagggaa
      661 gtaacaaaaa aactgcaaaa gtacaataat tatatatggc caaactaacc tctattaaaa
      721 aacatcagtt tttcacactt agttgagctt catgaaaaaa tcatcgagga tttcattgca
      781 gaatacacat tccactaact ggaagcactc cataagagga atcagagaaa gtatcgtttt
      841 tttatagtaa aattcattgc tgttcaataa aattgatata gttattaaat tatgaaaata
      901 atttcacaaa aaagattaac ttgagttctt aagcatcgga ttttagatat tttgaaatat
      961 ctggcatcac ataacatcaa aagtatataa tacccttatc taattacatg cagtatattg
     1021 tatatatact caaaaattgt aattcatacc ctgctatata ttgtagggaa agatattaat
     1081 taaaactgtt ttcatataag gttcatttta tgaatatatc agaaacactg aactcagcaa
     1141 atacccaatg caatatagat tctatggata acagattaca tacattgttt ccaaaagtga
     1201 catcagtgcg aaacgctgca caacaaacta tgccagatga aaaaaattta aaagatagtg
     1261 caaatattat taaagatttc tttaggaaaa ctatagcagc acagagttat agtagaatgt
     1321 tctctcaagg ctctaacttt aaatctttaa atatagcaat tgatgcacca tcagacgcta
     1381 aagcctcatt taaggctatt gagcaccttg acagattatc gaagcattat atatctgaaa
     1441 taagggaaaa acttcatcct ctttctgcag aggaactcaa tttgctttcg ctaattatta
     1501 attctgattt aatcttcaga catcaaagta attctgattt gtctgataaa attttaaaca
     1561 ttaagtcatt caataaaatt cagtctgaag gaatatgcac aaaacgaaac acatacgctg
     1621 atgatataaa aaaaatagct aatcatgact ttgtgttttt tggcgttgaa atctctaacc
     1681 atcagaaaaa acaccccctg aatacaaaac atcacactgt tgattttggt gcaaatgcgt
     1741 atatcattga tcatggctct ccatatggat atatgacatt aaccgatcac tttgataatg
     1801 ctattccacc tgttttttac catgagcacc aatcattttt agataaattt tcagaggtta
     1861 ataaagaagt tagtcgatac gtacatggaa gtaaaggaat tatagatgta ccaatattca
     1921 atactaaaga tatgaagtta gggctcggat tatacctgat tgactttatt agaaaaagtg
     1981 aagaccaaag cttcaaggag ttttgctatg gaaaaaatct tgcccctgtg gatctggata
     2041 gaatcataaa ctttgttttt cagccagagt accatatacc taggatggta agtacagaaa
     2101 acttcaaaaa agttaagatt agagaaatat ctttagagga ggctgttaca gcatctaatt
     2161 acgaagaaat taacaagcag gtcactaaca aaaaaattgc tctccaggct ctttttcttt
     2221 cgattactaa tcaaaaagag gatgtcgcct tatatatatt atctaatttt gagataacta
     2281 gacaagatgt tatttccata aagcatgagt tgtatgatat tgagtatcta cttagcgctc
     2341 ataattcaag ctgtaaagta cttgagtatt ttatcaataa gggattggtt gatgtaaaca
     2401 caaagttcaa aaaaactaat agtggggatt gtatgttgga taacgcaata aaatatgaga
     2461 atgcagaaat gataaaacta ttattgaaat atggtgcaac atctgacaat aaatatattt
     2521 aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc acaaaatatc gatatattgt
     2581 ttattgtcag tatggctcaa tgtgataata gttggaaagt ttgatgggtt tcgccccgtt
     2641 gtagcggtag tcgaactgga aggtcttcag gcactgctta cagcgataga gcagcccccc
     2701 tgaactggaa tggccgttcc ggataccccc ctgagtttca gagtaacggg gacaaaccac
     2761 atcaatcttt gccatcaatc atccaaaggg caaagagtac aacaacacta agtctgcgtc
     2821 acaacccatc aatgaaagga atatatacat atgccatcag taaatttaat cccatcaagg
     2881 aaaatatgtt tgcaaaatat gataaataaa gacaacgtct ctgttgagac aatccagtct
     2941 ctattgcact caaaacaatt gccatatttt tctgacaaga ggagtttttt attaaatcta
     3001 aattgccaag ttaccgatca ctctggaaga cttattgtct gtcgacattt agcttcctac
     3061 tggatagcac agtttaacaa aagtagtggt cacgtggatt atcatcactt tgcttttccg
     3121 gatgaaatta aaaattatgt ttcagtgagt gaagaagaaa aggctattaa tgtgcctgct
     3181 attatttatt ttgttgaaaa cggttcatgg ggagatatta ttttttatat tttcaatgaa
     3241 atgatttttc attccgaaaa aagcagagca ctagaaataa gtacatcaaa tcacaatatg
     3301 gcattaggct tgaagattaa agaaactaaa aatggggggg attttgtcat tcagctttat
     3361 gatcccaacc atacagcaac tcatttacga gcagagttta acaaatttaa cttagctaaa
     3421 ataaaaaaac tgactgtaga taattttctt gatgaaaaac atcagaaatg ttatggtctt
     3481 atatccgacg gtatgtctat atttgtggac agacatactc caacaagcat gtcctccata
     3541 atcagatggc ctaataattt acttaacccc aaagttattt atcacgcgat gcgtatggga
     3601 ttgactgagc taatccaaaa agtaacaaga gtcgtacaac tatctgacct ttcagacaat
     3661 acgttagaat tacttttggc agccaaaaat gacgatggtt tttcaggatt gcttttagct
     3721 ttacaaaatg ggcattcaga tacaatctta gcatacggag aactcttgga aacttctgga
     3781 cttaaccttg ataaaacggt agaactacta actgcggaag gaatgggagg acgaatatcg
     3841 ggtttatccc aagcacttca aaatgggcat gcagaaacta tcaaaacata cggaaggctt
     3901 ctcaagaaga gggcaataaa tatcgaatac aataagctga aaaatttgct gaccgcttat
     3961 tattatgatg aagtacacag acagataccc ggactaatgt ttgctcttca aaatggacat
     4021 gcagatgcta tacgcgcata cggtgagctc attcttagcc cccctctcct caactcagag
     4081 gatattgtaa atttgctggc ctcaaggaga tatgacaatg ttcccggact tctgttagca
     4141 ttgaataatg gacaggctga tgcaatctta gcttatggtg atatcttgaa tgaggcaaaa
     4201 cttaacttgg ataaaaaagc agagctgtta gaagcgaaag attctaatgg tttatctgga
     4261 ttgtttgtag ccttgcataa tggatgtgta gaaacaatta ttgcttatgg gaaaatactt
     4321 cacactgcag accttactcc acatcaggca tcaaaattac tggcagcaga aggcccaaat
     4381 ggggtatctg gattaattat agcttttcaa aataggaatt ttgaggcaat aaaaacttat
     4441 atgggaataa taaaaaatga aaatattaca cctgaagaaa tagcagaaca cttggacaaa
     4501 aaaaatggaa gtgattttct agaaattatg aagaatataa aaagctgaat attatctcgt
     4561 ggtgatacac tgaatttgag gtaaccggct catttaaacc gtctggtctg ttacctccgg
     4621 ttccacaaaa ataatgtcca tcattttgaa gtggacacta tcgtatggaa taccggacct
     4681 ggatcactga agctttacgc cttcatttcg aagaacattt accacgggtt gtggccggac
     4741 gtcgcttggg cgtaccaaaa tcaactgtct gtggtatgtt cgtgtgaacc gcccccggta
     4801 tcccgctcat gctggcgaac ccagacacgc agtgaaccgc cccgggtttc ctggagagtg
     4861 ttttatctgt gaaccacggc agcctggcgg ctgcaaatcg gggtgtggca gaatatgagc
     4921 tttcagagta accatccaga atgagttccc atgtcacaca acctggagca ccagaaagtc
     4981 cacaccagaa tggtgaaaga ggttctgaaa gccgtcgcca gagccaataa tcacccttat
     5041 aagtccgtat ttgcagactt tatcaccggt catccctcct gcacggtctg tttctgggaa
     5101 accttccaca aaatgtaccc ggattcaccg tacgaatacg tcacgttctg ccatacctgc
     5161 cgtcgttttg atttatacga aacagaagcg gagatgaagg cggatgaccc gaagtggtgg
     5221 tgattt
//
LOCUS       DAJTYG010000177         5213 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTYG010000177 DAJTYG010000000
VERSION     DAJTYG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.178"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(238..576)
                     /gene="osmE"
                     /locus_tag="OO568_003831"
     CDS             complement(238..576)
                     /gene="osmE"
                     /locus_tag="OO568_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6642964.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            778..1605
                     /gene="nadE"
                     /locus_tag="OO568_003832"
     CDS             778..1605
                     /gene="nadE"
                     /locus_tag="OO568_003832"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6642965.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            1835..2722
                     /gene="cho"
                     /locus_tag="OO568_003833"
     CDS             1835..2722
                     /gene="cho"
                     /locus_tag="OO568_003833"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6642966.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(2682..3257)
                     /gene="ves"
                     /locus_tag="OO568_003834"
     CDS             complement(2682..3257)
                     /gene="ves"
                     /locus_tag="OO568_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6642967.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLWLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3460..3945)
                     /gene="spy"
                     /locus_tag="OO568_003835"
     CDS             complement(3460..3945)
                     /gene="spy"
                     /locus_tag="OO568_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6642968.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4275..5198)
                     /gene="astE"
                     /locus_tag="OO568_003836"
     CDS             complement(4275..5198)
                     /gene="astE"
                     /locus_tag="OO568_003836"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003840897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6642969.1"
                     /translation="MTEREINGVRWRWLGDGVLELTPLTPPQGALVISAGIHGNETAP
                     VEMLDALLGAISHSEIPLRWRLLVILGNPPALKQGKRYCHSDMNRMFGGRWQLFAESG
                     ETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGVLPQRDIPWDEKFLTW
                     LGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFGQNDLRQFAVTASAIA
                     ALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFMPFEKGTLLAQDGEER
                     FTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         1306 a         1297 c         1264 g         1346 t
ORIGIN      
        1 catgctctgg gtaacttgcg aaaccaacat gacttttttt gtggctgaaa ggagtatacg
       61 gtatagacct gagcaatagt atttctttga ccttataaat tgtttctcct tgacctgtgg
      121 ttatgaccct cttcacattt cgggcaaata attcgcgaag ataattaaga actcaggcca
      181 aaaaaaaccg gcgcaatggc cggtttccgt tgttactcaa gctttcagac gaattgatta
      241 cttcgcagcc tgtggatcag tgtcgtattc agcacaggtc tgataaccgg agttgatgac
      301 atgtccggta tcatctaacg cgacaaagta ggtttctgct ttaccatcac gttgacccag
      361 gatgtaggtc tggcaagtac cgcgagcatg gatcatgctc acttcagacg aaggtttacc
      421 cgcaatttgt gcaacctgcg cccggctcat gccttttttg acgtctttca ccacaggctg
      481 tacaaactgg tctttggtac gatcataagc tgtacaaccc gccagcatgg ttaataccgc
      541 cgctgcactc agaattcctg ccatattctt gttcatattc cgtcctcttg tttatcagcg
      601 tgttagataa gcctggaata cattgggcgc tttttcaagc ccgtgaacga aacggctccg
      661 ctttcagagg attcctgtat gacgttttaa ccaccattca gcccgctgtc gcttgtcgtt
      721 tcagtagcaa cgggttagct ttaaggaagt tttgtctttt ctgtctggag gggttcaatg
      781 acattgcaac aacaaataat aaaggcgctg ggcgcaaaac cgcagattaa tgctgaagag
      841 gaaattcgtc gtagtgtcga ttttctgaaa agctacctgc aaacttatcc gttcattaaa
      901 tcactggtgc tcgggatcag cggcggtcag gactccacgc ttgccggaaa gctgtgccag
      961 atggcgatta atgagctgcg ccaggaaact ggcaacgaat cactgcaatt tattgccgta
     1021 cgcctgccct atggtgttca ggctgacgaa caagattgcc aggatgccat tgcctttatt
     1081 caaccggatc gcgtattaac cgttaatatc aagggcgcgg tattggctag cgagcaggca
     1141 ttgcgggaag caggcattga actgagcgat tttgtccgtg gcaatgaaaa agcgcgtgag
     1201 cggatgaaag cacaatatag cattgcgggt atgaccagcg gtgtcgtggt gggcaccgat
     1261 catgcagcag aagccattac cggattcttc actaaatatg gtgacggcgg tacggatatt
     1321 aatccgctgt atcgtctcaa caaacgccag ggtaaacagt tactgacggc attaggttgc
     1381 ccggaacacc tttataagaa agccccaact gccgatcttg aagatgatcg cccttctctg
     1441 ccagatgaag tggcactcgg cgtgacctat gacaatatcg acgactatct ggaagggaaa
     1501 aacctacctg aacaggtcgc cagaacaata gagaactggt atctgaaaac cgaacataaa
     1561 cgccgtccgc caattaacgt tttcgatgat ttctggaaaa agtaataatt tgcacctatt
     1621 ggattgtgcg aaaaagagta atttgttcac gccggatgcg gcgtgaacgc cttattcgac
     1681 ctacaaaact atgcaaattc aatacattgc aggagtcgaa taggcctgac aggcgtagca
     1741 cgtcagacgg tgtaaccttt gtcatcaacc cgcctctttt ttaatcgcct cccgcctgtt
     1801 acactggata gataaccagc attcggagtc aacagtggta cggcgtttaa cttctccgcg
     1861 gctcgaattt gaagctgcgg caatttatga atatcccgaa catttacatt cattccttaa
     1921 tgacttaccc acccgaccag gggtgtatct gtttcatggc gaaagtgaca ccatgccgct
     1981 ctatatcggc aaaagcgtta atatccgcag tcgggtgctt tctcatttac gcaccccgga
     2041 tgaagccgcc atgctacggc aatcccggcg aattagctgg atctgcaccg caggcgaaat
     2101 cggcgctctg ctccttgaag cgcgattaat caaagaacaa cagccgctgt ttaataaacg
     2161 gttgcgccgc aatcgccagc tctgtgccct gcaattaaat gaaaagcgcg tcgatgtggt
     2221 gtatgccaaa gaggtggatt tttcacgagc ccccaacctg tttggcctgt ttgccaatag
     2281 gcgcgcagct ttgcaagcat tgcagagcat cgctgatgaa caaaaacttt gttatggcct
     2341 gttgggactg gaaccgttaa gtcgcggtcg tgcatgtttt cgttcagcgc taaaacgttg
     2401 cgccggagca tgctgcggta aagagagcca tgaggaacat gcgctacgct tgcgccagtc
     2461 tctggagcgt ttgcgggtgg tgtgttggcc ttggcaaggt gcggtggcac tgaaagaaca
     2521 gcacccggaa atgactcaat atcatattat tcaaaactgg ctgtggctgg gggcggttaa
     2581 ttcgctggaa gaagcgacaa cgttaattcg gacacccgcc gggtttgatc acgacggtta
     2641 taaaattctt tgtaagccgc tgctttccgg taactatgaa attactgaac ttgatccggc
     2701 gaatgaccag cgagccagtt gatttcactg aacaacagtt taccttgcgg ttgtaaaagc
     2761 cacagggtgt gcctgccatc gaaccagcac gcgccctggt cggtagtgag cagtttgtcg
     2821 cccagttgcc acgccccgtt aatcacaaac accaccccgc cgcgtgagcc aaaggtagta
     2881 aacgtacgtt cggcaattct gactttcgcc ttacagacat ccaggcgcgt cataatattg
     2941 aaatccatcg acatctgccc tgccgtcagt ttcgctttta ccacctggtc cgccgtaaag
     3001 gcaaaaggct gtaacggttt taaggtatgg ttaaagcggt ctgcgctttc aaggagcatc
     3061 tcgccgcctt ccagcaacgt cactatcctt tccatgccgg gaaacaaaga aaactcccca
     3121 ttcgccgcga tggatgcaat gctggcacgc cagtaaaaat cacgttttgc cggtgggaaa
     3181 gtgcaaattt cgcgcgtttc accggcagcg tttcgccaca gattcaccga cattttacgc
     3241 atatcaaagt attccatact cgctcccggc ccgttctttt cattgttatc gttcaggtaa
     3301 ccgtggatgg ttatcggcaa ctcgggtgat ttacgttagt ggtgatcagg ctttttttac
     3361 aactgtttga gaagagaaaa gaaaaccgcc gatcctgtcc accgcattac tgcaaggtag
     3421 tggacaagac cggcggtctt aagttttttg gctgaaagat tattcagcag ttgcaggcat
     3481 tttacctttt gccgctggac gttctgtcag acgcttctca aaattagcat taaattgctt
     3541 tttctgttcc ggcgtcagga tgttgtaaat tttgttctgg gtttccatgt gcgccagcat
     3601 gttagctttg cgctgttctt ccatttttgc gatctgcgct tcagcttttg ctttatcgaa
     3661 ggtatcgctg gcaatgatgt catgcattgc gcggcgttct tccagcggcg gacgtttcat
     3721 ctggtcacgc tggcctttca tgatttcgtg gatctgctgt ttctgcgcgt cggtcaggtt
     3781 caggtctttg aacatcatgt cctgatgcgg accgaacttg cctttgtggt gcatcatcgg
     3841 cttcgcgtca gccggtgctg cggtagtggt gtctgcggca tgggccaggt tagccgcgcc
     3901 aagagccagg gtagaggcaa caaacagtgc agttaattta cgcatattct atatccttcc
     3961 tttcagttat ttattacggc tttcttaagt agcgtgccgt gttgacgaga ttaactttac
     4021 tgactttagc gtcaattaat cagagcaacg gtaaaacaat gaaagtgtaa aaaacacttt
     4081 tgcgccaatt atggagaaaa aaagaaaatt tgatggagag tgatgagaga atattacaac
     4141 acgatgattt tgcagagatt atgaagaact ataccggatg actggtgata aataaagcaa
     4201 ataaccagga ttaatctgta ttaatttata agaaagcaac ttaatacccg cagaatgatt
     4261 tctgcgggta agtattagct tattttttcg agcattaatc ccgcgcgtaa tcccaacgct
     4321 accaacggat tagggaataa cacatactct acatcatggg ttacggtaaa acgttcctct
     4381 ccgtcctgcg ccagcaatgt tcctttctca aacggcataa aattcagcgt gtcacttgcc
     4441 atatgcattt cgaaggacgg cgagtggcga gtaatttgcg aaaccacccg ataacggagc
     4501 ggcggtgttc tcacgatacc gacactctca ccagatagca gcgcagcaat tgcgctggca
     4561 gttactgcaa actggcgaag atcgttttgc ccaaagggca acgctttgcc aagttccaac
     4621 gtacaggcca gcgcgccaaa atgtctgacg ctgaaatggg taaacgtacc accaggctcc
     4681 tgatggaaca ccagcgcctc cagccccgcc gcacccagcc acgtcagaaa tttctcgtcc
     4741 cagggaatgt cgcgttgcgg taatacaccg aactgcggat gcaaggagcc acgaattgcg
     4801 gtatgtagat caaggtgcca acgcacagat tctttgccct ggtcataaaa atcttccagg
     4861 cactgttcca gttcgcgcgc ccgacaggtt tctccgcttt cagcaaatag ctgccaacga
     4921 ccgccaaaca ttcggttcat atcgctatgg caataacgtt tcccttgctt cagcgcagga
     4981 ggattcccga ggatcaccag caaccgccaa cgtaacggga tctcgctgtg agatatcgcg
     5041 ccaagcaacg cgtccagcat ttccacaggt gccgtctcat taccgtggat ccccgcagaa
     5101 atcaccagcg cgccttgcgg tggcgttaat ggcgtcagtt ccagcacacc atcgcccagc
     5161 cagcgccagc gaacgccgtt gatttctcgc tcggtgataa ccggtttttt acc
//
LOCUS       DAJTYG010000178         5152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTYG010000178 DAJTYG010000000
VERSION     DAJTYG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.179"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            250..1365
                     /gene="adrA"
                     /locus_tag="OO568_003837"
                     /gene_synonym="dgcC"
     CDS             250..1365
                     /gene="adrA"
                     /locus_tag="OO568_003837"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6642970.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1382..2191)
                     /gene="proC"
                     /locus_tag="OO568_003838"
     CDS             complement(1382..2191)
                     /gene="proC"
                     /locus_tag="OO568_003838"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6642971.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRVQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2311..2769
                     /locus_tag="OO568_003839"
     CDS             2311..2769
                     /locus_tag="OO568_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6642972.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2952..3476
                     /gene="aroL"
                     /locus_tag="OO568_003840"
     CDS             2952..3476
                     /gene="aroL"
                     /locus_tag="OO568_003840"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6642973.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPNQV
                     ISEIRSALAQTINC"
     gene            3526..3717
                     /gene="yaiA"
                     /locus_tag="OO568_003841"
     CDS             3526..3717
                     /gene="yaiA"
                     /locus_tag="OO568_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6642974.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3975..4652
                     /locus_tag="OO568_003842"
     CDS             3975..4652
                     /locus_tag="OO568_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6642975.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4724..5008
                     /gene="ppnP"
                     /locus_tag="OO568_003843"
     CDS             4724..5008
                     /gene="ppnP"
                     /locus_tag="OO568_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6642976.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT         1247 a         1225 c         1353 g         1327 t
ORIGIN      
        1 aattgtcgcc cattgtgagt catattcgcc ctgactttcc agaaccatac ggactgcccg
       61 ttgacggact tcgggggaaa aacgagtatt tttagtcatc ctgtttacct ctttctcagg
      121 aagtttagtc tccaggattc ccggggcggt tcagatagtg ctaacgggtg agctacgaaa
      181 gtggctcacc ctaaatttca tacttctgcc tttagccccg tctctataat ttgggaaaat
      241 tgtttctgaa tgttcccaaa aataatgaat gatgaaaact ttttcaaaaa agcggcggcg
      301 catggggagg aacctccttt aactcctcaa aacgaacatc agcggtccgg gctgcgcttc
      361 gcccgtcgcg tcagactacc ccgtgcggtt ggcctggctg gcatgttctt accgattgct
      421 tcaacgctgg tttcgcaccc gccgccgggc tggtggtggc tggtgttggt cggctgggcg
      481 ttcgtctggc cgcatttagc ctggcagata gcgagcaggg ccgtcgatcc gcttagccgg
      541 gaaatttaca acttaaaaac cgatgcagta ttagcgggaa tgtgggtagg cgtaatgggc
      601 gtaaacgtgc tgccttccac cgctatgttg atgattctgt gtctgaattt gatgggggca
      661 ggcggccccc gtctgtttgt cgcgggtctg gtgttgatgg tggtttcctg ccttgtcacc
      721 ctcgagctta cgggcattac cgtgtcgttc aatagtgcgc cgctggaatg gtggctctcc
      781 cttcccatta ttgtcgttta tcctctgctg tttggctggg tcagctacca gacggcaacc
      841 aaactggcgg aacataaacg caggttgcag gtcatgagta ctcgcgacgg catgacgggc
      901 gtgtataacc gacgtcattg ggaaactatg ttacgcaatg aatttgataa ctgtcggcgg
      961 cataatcgcg atgcaacgtt actgattatc gatatcgacc atttcaagag catcaacgat
     1021 acctggggtc atgatgtggg cgatgaagcg attgtggcgc ttacccgaca gttacaaatt
     1081 accctgcgcg gtagcgatgt gattggtcgg tttggcggcg atgagtttgc agtaatcatg
     1141 tccggtacgc cagctgagag cgccattacc gccatgttac gggtgcgtga agggctaaac
     1201 acgttacgct tgccgaatac gccacaggta actttacgga ttagtgtggg ggttgcgccg
     1261 ctgaacccac aaatgagtca ctatcgtgag tggttgaaat cggcagattt ggcgctttac
     1321 aaagcaaaga aagccggacg taaccgcacc gaagtggcgg cctgacgtcc ggcgaaagtc
     1381 atcaggattt gctgagtttt tctgattttt ccatacactt cgtcatcgct tcgatcactg
     1441 cggcacggaa gcctttctct tccagtacgc gtaccgcttc aatggtggta cctcccggtg
     1501 agcagaccat atctttcagt gcccccggat gttctcccgt ttccagcacc atttttgcgg
     1561 aacccattac cgcctgagcg gcaaatttat acgcctggac gcgtggcatc ccgcccagca
     1621 cggcggcgtc ggccatcgct tcgataaaca taaatacgta ggctggcgaa gaaccgctca
     1681 caccgaccac cgggtggatc atcggctcag caattacttc cgcttcgcca aagcagcgga
     1741 aaatattcag cacatcagcg gtatcttctg gggttaccag cgcgtttggc gttacggagg
     1801 tcatcccggc attaaccagc gcgggagtgt tcggcatggc gcggataatt ttccggtcat
     1861 ggcccagcgc gcgggcaagc tggtcgagcg tgacacctgc agcaatagaa acgaccagag
     1921 agtctttatt caggctggag gtgatttcgc taagcacttt aatcatgatg ccaggtttaa
     1981 cggcagcaaa aatgatgtcg gcgatttgcg ccacttcttg cgccgattct gcggcgttga
     2041 tgccgaactg gtcatgcagg gcggcgactt tatccgggga gggggtgtat acccagattt
     2101 gccctggaag cacctgaccg ctggcaatca gaccgccgag aatggctttt cccatattgc
     2161 cgcagccaat aaaaccgatt ttcttttcca ttgcctcact cctgccgtga aattcattgt
     2221 tttgataatc gctggcagaa gcataaacag aactatgccg gaaggcaaaa gcgcgacaca
     2281 atagaggatt acccaacaaa ggatgacttt atgacaattt gggtggatgc cgacgcgtgt
     2341 cccaatgtaa ttaaagagat tttgtatcgc gcggcggaac gtatgcagat gccgctggta
     2401 ctggtagcaa accagagttt acgcgtgccg ccatcgcgat ttattcgtac gctgcgcgtc
     2461 gcggcaggtt tcgacgttgc cgataacgaa attgtccggc agtgtgaagc gggcgatttg
     2521 gtgatcaccg cagatatacc tttggctgct gaagccatcg agaaaggcgc agcggcgctt
     2581 aatccgcgcg gcgaacgtta cacgccagcg accattcgtg agcgcctgac gatgcgcgat
     2641 tttatggata ccttacgtgc cagtgggatc cagaccggcg gaccagatag cctttcacaa
     2701 cgtgaccgcc aggcctttgc cgcggagctg gagaagtggt ggctggaagt gcaacgtagt
     2761 cgtggctaaa tgtaatttat tatttacact tcattcttga atatttattg gtatagtaag
     2821 ggatgtattg agattttcac tttaagtgga attttttctt tacaatcgaa attgtactag
     2881 tttgatggta tgatcgctat tctcatgaca ccggctttcg ccgcattgcg acctattggg
     2941 gaaaacccac gatgacacaa cctctttttc tgatcgggcc tcggggctgt ggtaaaacaa
     3001 cggtcggaat ggcccttgcc gattcgctta accgtcggtt tgtcgatacc gatcagtggt
     3061 tgcaatcaca gctcaatatg acggtcgcgg agatcgtcga aagggaagag tgggcgggat
     3121 ttcgcgccag agaaacggcg gcgctggaag cggtaactgc gccatccacc gttatcgcta
     3181 caggcggcgg cattattctg acggaattta atcgtcactt catgcaaaat aacgggatcg
     3241 tggtttattt gtgtgcgcca gtatcagtcc tggttaaccg actgcaagct gcaccggaag
     3301 aagatttacg gccaacctta acgggaaaac cgctgagcga agaagttcag gaagtgctgg
     3361 aagaacgcga tgcgctatat cgcgaagttg cgcatattat catcgacgca acaaacgaac
     3421 ccaaccaggt gatttctgaa attcgtagcg ccctggcaca gacgatcaat tgttgatttt
     3481 agagcgccta tacttaacgt tcatcccgtg aaataaggaa gaacgatgcc aacgaaaccg
     3541 ccttatcctc gtgaagcata tatagtgacg attgaaaaag gaaagccagg acagacggta
     3601 acctggtacc aactcagagc cgatcatcct aaaccagact cgttgatcag tgaacatccg
     3661 acggctcagg aagcgatgga tgcgaaaaaa cgctatgagg accctgacaa agagtgaccg
     3721 catcagactg ctcggaaggg attctgagtg ccactacaag ggatctgcgt cacatttttc
     3781 ataattcatg tttttctaat aattagaata ttaaacaata acattccatt actggaatca
     3841 tttggaatct ttacattatg ccgtgcacgt ctgctgctac gctttttgtc atttgtagca
     3901 caagtaagtg tcagcagtgg tgcttcacac ttgcccggta attaacgacg aaagaaaagt
     3961 aaggtggatg aacaatgagt gcgtcgttgg cgatcctgac catcggcatt gtacctatgc
     4021 aggaggtttt gccgctcctg acggaataca ttgacgaaga taatatttcc catcatagcc
     4081 tgctggggaa gttaagtcgt gaagaagtga tggcggagta cgcgccagaa gcaggcgaag
     4141 acaccattct cacattatta aatgacaacc agctggccca tgtttcgcgt cgtaaagtgg
     4201 agcgagatct gcaaggtgtg gttgaagtgc tcgataatca gggttatgac gtcattttat
     4261 taatgagtac agcaaacatt agtagtatga ctgcgcgtaa tacgatcttt cttgagccgt
     4321 cgcgaatatt gcctccactg gtttcctcta ttgttgaaga tcatcaggtg ggggttatcg
     4381 ttccggttga ggagatgctg cccgttcagg cgcaaaaatg gcaaattttg cagaaatcgc
     4441 cggtattttc attgggtaac cccattcatg attcagaaca aaaaatcatt gatgctggga
     4501 aagaattact ggcaaaaggg gctgatgtca tcatgctgga ttgtttagga tttcaccaac
     4561 gtcatcgcga tttactgcaa aaacagctcg atgttcctgt cttgctctct aacgtattga
     4621 ttgcacggct ggctgcggaa ttactggtgt aattttgcgt gacagccagc gcctctggcc
     4681 cctatagtga agtagatgtt caactaccag acagggccag tttatgcttc aaagtaatga
     4741 gtacttttcc ggcaaagtga aatcgatcgg cttttccagc agcagcactg gtcgcgccag
     4801 cgtgggtgtt atggttgaag gcgaatacac cttcagcacc gctgagccgg aagagatgac
     4861 ggtaatcagt ggcgcgctga atgtgttact gcctgacgcg accgactggc aagtgtatga
     4921 agccggttcg gtgtttaatg ttcccggtca cagtgagttt catcttcaag ttgccgaacc
     4981 tacctcttat ctgtgccgct atctgtaatt cctcgccttc cccttgaacg ggagggcatt
     5041 tttctgaaat atcctctctt taacccataa taatatttcc tttgctgcga ttttttcaat
     5101 ttccgatata ttcataattc atcaaggttg atataaatat cagtgaagat ct
//
LOCUS       DAJTYG010000179         5126 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTYG010000179 DAJTYG010000000
VERSION     DAJTYG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.180"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..236
                     /locus_tag="OO568_003844"
     CDS             <1..236
                     /locus_tag="OO568_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642977.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            276..816
                     /locus_tag="OO568_003845"
                     /pseudo
     CDS             276..816
                     /locus_tag="OO568_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            883..2301
                     /gene="dcm"
                     /locus_tag="OO568_003846"
     CDS             883..2301
                     /gene="dcm"
                     /locus_tag="OO568_003846"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6642978.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2282..2752
                     /gene="vsr"
                     /locus_tag="OO568_003847"
     CDS             2282..2752
                     /gene="vsr"
                     /locus_tag="OO568_003847"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6642979.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="OO568_003848"
     CDS             complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="OO568_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR6642980.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGEIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            3834..4751
                     /locus_tag="OO568_003849"
     CDS             3834..4751
                     /locus_tag="OO568_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6642981.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4830..5012
                     /locus_tag="OO568_003850"
     CDS             4830..5012
                     /locus_tag="OO568_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6642982.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
BASE COUNT         1253 a         1227 c         1462 g         1184 t
ORIGIN      
        1 gggctttcta tacatggtgt ggctatttcg tctgggcaat ggcgcgatac atctgggtga
       61 tgagccggat ccccgatgcc cccgtgtcgg gatttgaaag tgatttagga tctaccgcgg
      121 ggaagtggtt aggagcattg gtcggatttc tgtttatggc cctggttggt gccttgttag
      181 gcggcattgc atggtacacc cgacctcgtc ccgcacgttg cagacgctat gaataaaaac
      241 tgctaaggtt gcgactcttt ggcaacggag cgcgtatgga gttacaacac tggcaggcac
      301 agttcgagaa ctggttgaaa aaccatcatc aacatcagga cgcggcgcat gacctgtgtc
      361 attttcgccg tgtctgggct acggcacaga aactggcggc agatgacgat gtcgatatgc
      421 tggtgatttt aaccgcctgt tattttcacg atatcgtaag cctggcgaaa aatcatccgc
      481 aacggcagcg ttcttcaatc ctggcggcag aagagacgcg tcgcctgctc cgtgaagagt
      541 tcgtgcaatt tccggcggag aaaattgagg ccgtttgtca tgccattgcc gctcacagtt
      601 tcagcgcgca aatcgccccc ttaacaacgg aagctaaaat cgtccaggat gcggatcggc
      661 tggaagccct gggggcgatt ggcctggcgc gcgtatttgc cgtttcaggg gcattgagct
      721 tggcgctgtt tgatggcgaa gctccgtgcc gaactggcgg gggagaatga aggtatcgat
      781 cacaaggtga tagatgcgtt ttcacccgct ggctgagcgc gtggccctaa acggcagtaa
      841 ttatgttaac ctgtcggcca tctcagatgg ccggttaaat ctatgcagga aaatatatca
      901 gtaaccgatt catacagcac cgggaatgcc gcacaggcaa tgctggagaa actgctgcaa
      961 atttatgatg ttaaaatgct ggtggcgcag cttaatggtg tgggtgagaa tcactggagc
     1021 gcggcaattt taaaacgtgc gctggcgaat gactcggcat ggcaccgttt aagtgagaaa
     1081 gagtttgccc atctgcaaac gttgttaccc aaaccaccgg aacatcatcc gcattatgcg
     1141 tttcgcttta tcgatctatt tgccggaatt ggcggcatcc gtcgtggttt tgaatcgatt
     1201 ggtggacagt gcgtgtttac cagcgaatgg aacaaacatg cggtacgcac ttataaagcc
     1261 aaccattatt gcgacccggc gacgcatcat tttaatgaag atatccgcga catcaccctc
     1321 agtcatcaag aaggcgtgag tgatgaggcg gcggcggaac atattcgtca acacattcct
     1381 gaacacgatg ttttactggc cggtttccct tgtcagccat tttcactggc tggcgtatcg
     1441 aaaaagaact cgctcgggcg ggcgcacggt tttgcctgcg atacccaggg cacgctgttc
     1501 tttgatgtgg tgcgcattat cgacgcgcgt cgtccggcga tgtttgtgct cgaaaacgtc
     1561 aaaaacctga agagtcacga taagggaaaa acgttccgca tcatcatgca gacgctggac
     1621 gaactgggct atgacgttgc cgatgcagaa gataacgggc cggacgatcc gaaaatcatc
     1681 gatggtaaac atttcctgcc gcagcaccgt gaacgcatcg tgctggtggg ttttcgtcgc
     1741 gatctgaatc tgaaagccga ttttactctg cgtgatatca gcgaatgttt ccctgcacag
     1801 cgagtgacgc tggcgcagct gctggacccg atggttgagg cgaaatatat cctgacgccg
     1861 gtgctgtgga agtacctcta tcgatatgcg aaaaaacatc aggcgcgcgg taacggcttc
     1921 ggttatggaa tggtttatcc gaacaatccg caaagcgtca cccgtacgct gtctgctcgt
     1981 tattacaaag atggcgcgga aattttaatc gatcgcggtt gggatatggc cacgggtgag
     2041 aaagactttg acgatccgct gaatcagcaa catcgtccac gtcggttaac gcctcgtgaa
     2101 tgcgcgcgct taatgggttt tgaagcgccg ggagaagcga aattccgcat tccggtttcg
     2161 gacactcagg cctatcgcca gttcggcaac tcggtggtcg tgccggtctt tgccgcggtg
     2221 gcaaaactgc ttgagccaaa aatcaaacag gcggtggcgt tgcgtcagca agaggcacaa
     2281 catggtcgac gttcacgata aggccacccg cagcaaaaat atgcgcgcga ttgccacgcg
     2341 tgatacggcg attgagaagc gcctcgccag tttgttaacc gggcagggcc ttgcgtttcg
     2401 cgttcaggac gccagtctgc ccggacgccc ggattttgtc gttgatgaat atcgctgcgt
     2461 gatatttacc cacggctgct tctggcatca tcatcactgc tatctgttta aagtgcctgc
     2521 gactcgaact gagttctggc tggagaagat aggtaaaaat gttgaacgcg atcgccgcga
     2581 tatcagtcgc ttgcaggagc tcggctggcg cgtactgatt gtctgggagt gtgcgttacg
     2641 taggcgcgag aagctgacgg atgcggcgct taccgagcgt ctggaagagt ggatctgcgg
     2701 cgaaggtgcc agcgcgcaga tcgacacgca ggggattcat ttactcgctt gatgcatcct
     2761 gaataactgg cgcgacaacg ggtttggccg ggaagagata ttttcccaac gtgaccagta
     2821 ccaccgcgaa aacaattacg ccgagcgcca gccattcaat cttcgacagc gtttcgccac
     2881 ccagtcccgt acccagcaag accgcgacca ccgggttaac gtaggcgtag ctggtggcga
     2941 gagccggact gacattacgg attaaataca tataagcgtt aatggcgata atcgaaccaa
     3001 acagcgccag atagccgacc gcaaggaagc ctgacaggga agggagcgcc gtcagttttt
     3061 cacccgcaat catcgatgcg atcattaaca ccacgcctgc cgccagcatc tcaatctcac
     3121 ccgccatcat ccctacaggt aaggtaatgc gcgagccata aactgagcca aacgcccagc
     3181 taatcgagcc gattaaaatc agaatcgcgc cccacggatt gccgcttaaa tttccaccgc
     3241 tattgagcat gatgattccg gcaagcccaa tggcaatacc cacccattcc agtttgcgcg
     3301 ttttaatgcc aaacaggcga ctgaagcaca gggtaaagag gggcacggtt gcaatcacta
     3361 cggcggcgat gccggaagga acattttgat gttcggcaac cgtcaccatg ccattaccga
     3421 cagccagcaa taacaggcca atcagcgcgg cattgagcag cggacgtagc gggggaagtt
     3481 tgtgtccgcg cagtagcaaa aatgccagca ataaaatacc ggctgccagg aatcgaacgc
     3541 ccgccatcat taacggaggc cagcttccca cgccaatccg aatgacaaaa taggttgagc
     3601 cccaaatgat atacaacgca aacagagcgc caaaaagcgg taacaactgg cggaaacgca
     3661 taatccctca cggcggaaat aaaaaggtgg ttcatagtaa acgtgaaaat cattctgctg
     3721 gcgagagata taattgcact tgattgttaa ataaatgttg acctgtgaag caagtatcag
     3781 agctgccgtt tttgcttcat acttacaccc ttcagcaata aaaatgagag ggaatgcttt
     3841 tggccgggag tagtttactg acgttgctcg atgatatcgc cacactgctg gacgatatct
     3901 ctgtgatggg caaactggcg gcgaagaaaa ccgccggtgt attaggggat gatttatcgc
     3961 tcaatgcgca acaagtttca ggcgtgcggg ccaaccggga acttcccgtg gtctggggcg
     4021 tggcgaaagg atcgctgatc aataaagtga ttctggtgcc gctggcgctg atcatcagtg
     4081 cgtttatccc gtgggcgatt acgccactgt tgatgattgg tggcgcgttt ctctgctttg
     4141 aaggggtaga gaaagtgctg catatgctgg aggcgcgtaa acataaagaa gatccagcgc
     4201 agagccagca gcgtctggag aagctggcgg cgcaggatcc gctgaagttt gaaaaggaca
     4261 aaattaaagg ggcgattcgt accgatttta ttttgtctgc ggaaatcgtc gccatcacgc
     4321 tggggattgt ggcagaggcg ccgttgctta atcaggtgct ggtgctttca ggcatcgcgc
     4381 tggtagtgac cgtgggcgtc tacggtctgg taggggttat cgttaagatt gatgacctag
     4441 ggtattggct ggcggaaaaa tccagcgcgc tgatgcaggc attaggtaaa gggttattga
     4501 ttatcgcgcc ttggctgatg aaagcattat cgattgtcgg cacgctggcg atgttcctcg
     4561 tcggcggcgg gattgtggta catggtattg cgccgctgca tcacgctatt gaacatatcg
     4621 ccgggcagca aagtgcagtg gtggcgatga tattaccgac tgttttaaat ctgattcttg
     4681 gatttatcat cggcggcatc gtggtgctgg gagtgaaagc agtagcgaaa atgcgcggtc
     4741 aggcacatta atactcgcac tggctattat gcaaaattcg ccatcttcaa ctaaggtgaa
     4801 caagtttcac tcgaaaaaag gaggcgagta tgagctttat ggttagtgag gaagttacgg
     4861 ttaaagaggg cggcccgcga atgattgtca ctggatactc cagcggtatg gttgagtgtc
     4921 gttggtatga cggttacggg gtcaagcggg aagcttttca tgaaaccgat cttgttccgg
     4981 gggaggggag tcgttctgcg gaagaagttt gaaagggtat cattaacacc aggccggatc
     5041 acatccggcc tggtgtccca atgcctgcaa tgaatgatag tcgcgtctta tcatgccacc
     5101 attataaaat tatcgctgtt ttgcgg
//
LOCUS       DAJTYG010000180         5116 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTYG010000180 DAJTYG010000000
VERSION     DAJTYG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.181"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..366)
                     /locus_tag="OO568_003851"
     CDS             complement(<1..366)
                     /locus_tag="OO568_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6642983.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLIIKNSTIHGMIYSECMTT"
     gene            892..1866
                     /gene="hemB"
                     /locus_tag="OO568_003852"
     CDS             892..1866
                     /gene="hemB"
                     /locus_tag="OO568_003852"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6642984.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1972..2823)
                     /gene="tauD"
                     /locus_tag="OO568_003853"
                     /pseudo
     CDS             complement(1972..2823)
                     /gene="tauD"
                     /locus_tag="OO568_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(2820..3647)
                     /gene="tauC"
                     /locus_tag="OO568_003854"
     CDS             complement(2820..3647)
                     /gene="tauC"
                     /locus_tag="OO568_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6642985.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVQALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3644..4411)
                     /gene="tauB"
                     /locus_tag="OO568_003855"
     CDS             complement(3644..4411)
                     /gene="tauB"
                     /locus_tag="OO568_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6642986.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVQERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4424..>5116)
                     /gene="tauA"
                     /locus_tag="OO568_003856"
     CDS             complement(4424..>5116)
                     /gene="tauA"
                     /locus_tag="OO568_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6642987.1"
                     /translation="VPIEVFLLASKLGNSEALVVKKTISKPEDLIGKRIAVPFISTTH
                     YSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDGAYVWAPAVNALEKDGKVLTD
                     SEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAIDAQQPYIANPDAWLKQPENIS
                     KLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNKAIIDTAQFLKEQGKVPAVAN
                     DYSQYVTSRFVQ"
BASE COUNT         1241 a         1409 c         1273 g         1193 t
ORIGIN      
        1 agttgtcatg cactcggaat aaatcatacc gtgaatagtg ctgtttttga ttatcagact
       61 attggcgttg gtattggtag aaataccgtc gtccagatag taggtagaaa tcacgccgtt
      121 aacggtagcg ttattaatga ccgggtagat atcgccgttg taatagctat cagcggcata
      181 gttgttccag cctacataac catcatagta aacgtcatca gcataggtgg cgtcgttgta
      241 gtgatggaaa gtattgtaag tttgaccaga aatatcgttg gtcgctgcat tagcctgaga
      301 ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga
      361 gtgcatttgt catccctcct cagggacgta ataagttgat ccatcaatta tcaatgcata
      421 gaaaacatat tatgtttcgc gctaacgatt atgctgcgtt acagacgaat ggttcaatta
      481 ttctttcccc taagtccgaa tgtggacata tataatttat ggagaaaaac acgcatcaaa
      541 ttgataaaag gacgatattt cagcatgttg cagataatgt tatttttgat gcatacgcgt
      601 tataaatatc aaaaacacaa accagataaa tatcttatca atatacaaca aagtttgaat
      661 tattcaattt atagcagcac aaccaaacgc caaaaaaaat aataaccaag agctataaat
      721 aaagattaaa aatattttca gtgaatatga aattattgct atttctatta caaagatgtc
      781 ctggtctctc tttcattctc ttcttgtcaa catcgcgaca actttcgtaa aacactcctg
      841 ccccgcttca ggtatactgt gcaccttaat tccacaaaca tcaggcagac catgacagac
      901 ttaatccaac gccctcgtcg cctgcgcaaa tctcctgcgc tgcgcgctat gtttgaagag
      961 acaacactta gccttaacga cctggtgttg ccgatctttg ttgaagaaga aattgacgac
     1021 tacaaagccg ttgaagccat gccaggcgtg atgcgcattc cagagaaaca tctggcacgc
     1081 gaaattgaac gcatcgccaa cgccggtatc cgttccgtaa tgacttttgg catctctcac
     1141 cataccgatg aaaccggcag cgacgcctgg cgggaagatg gactggtggc gcgtatgtcg
     1201 cgcatctgca agcagactgt gccagaaatg atcgtcatgt cagacacttg tttctgtgaa
     1261 tacacttctc acggtcactg cggtgtgctg tgcgagcatg gcgtcgacaa cgacgcgact
     1321 ctggaaaatt taggcaagca agccgtggtt gcagctgctg caggcgcaga cttcatcgcc
     1381 ccttccgccg cgatggacgg ccaggtacag gcgattcgtc aggcgctgga cgctgcaggc
     1441 tttaaagata cggcgattat gtcgtattcg accaagttcg cctcttcctt ttatggcccg
     1501 ttccgtgaag ctgccggaag cgcattaaaa ggcgaccgca aaagctatca gatgaaccca
     1561 atgaaccgtc gcgaggcgat tcgtgagtca ctgctggatg aagcccaggg cgcagactgc
     1621 ctgatggtta aacctgctgg agcgtacctc gacatcgtgc gtgagctgcg tgaacgtact
     1681 gaattgccga ttggcgcgta tcaggtgagc ggtgagtacg cgatgattaa gttcgccgcg
     1741 ctggcgggtg ctatagatga agagaaagtc gtgctcgaaa gcttaggttc aattaagcgt
     1801 gcgggtgcgg atctgatttt cagctacttt gcgctggatt tggctgagaa gaagattctg
     1861 cgttaatttt tttcctctca gaaaggggga gttaacctgt tgaaagtacg gtgaacgccc
     1921 cctcacccta accctctccc caagggggcg agggaaccgt ccacactcgt atcaccccgc
     1981 ccgataaaac ggtttatccc caaggatcgt cgcccgatgc ataatccgcc gctgtggcag
     2041 gtaatcggca ttggcatagt gctgagtcac gcggttatcc caaatcgcaa tatcattcgg
     2101 ttgccagcgc caacgcacct gaaactccgg tttagtgata tgggcgaata aaaagcctaa
     2161 caaggcttcg ctctctttct cgctgacatc gacaattcgc gtagtaaagc cttcattcac
     2221 aaacagcgcc tgtttaccgc tcaccggatg cgttcgcacc accggatgta gcaacggagg
     2281 atttttcgcg accgcctcgc gccagcgttg atgttcctcc tcggttttgc ggtatttgta
     2341 ttccgggaat gatttacgga aatcatgctc cgcacgcagc cagctcagca gctggcggaa
     2401 gggaacagag agcgcctcat aggccgcaat accgctggtt cagagcgtat caccgccggt
     2461 cgaaggtaac tctttagctg ccagaatcgc ccctgcgggt ggcgtttcaa taaatgtcac
     2521 atcggtatgc cagttgtcgt tatccggcgg attatcgtta tgggtatcca gcacgatgat
     2581 ctcgtcaacc ccttcggcat gagggtaaac agggtgaata tgcagttcgc caaaacgctg
     2641 ggccagcgcg cgttgctgct gcggcgtaat agcttgatcg cgcagaaata ccacctgatg
     2701 gcgcagcacc gcatggtaaa gctgttcaaa ctgattatcg cttaacgggc gcgtcaggtc
     2761 agcacccgaa atttgtgcgc cgatatgcgg ccccagcggg gtaatgctca gacgttcact
     2821 cattgtactt ctccatgcca gggcgtcaga cggcgctgta acgcgcgcag acccagttct
     2881 aaaagaaagg cgataatcgc aatcaccgcg atccccgcca gtaccacgtc agtcgcgaga
     2941 aattcacccg ctgactgaac cataaatcct aaaccgcgtg tcgcggcaat cagctccgcc
     3001 gctaccagcg tagaccagcc cacacccaga ccaatgcgta atccggtgag gatttccggc
     3061 agcgcgccgg gcaaaatgac aaaccacagc acctgcgcac ggctggcacc cagcgactgg
     3121 gcggcacgaa tgcgaacctg ctgcgcgctt ttcacccctg ccagcgccga catcgccacc
     3181 ggcgcaaaaa tcgctaaata gatcagtaag atcttcgagg tttcaccaat accaaaccag
     3241 atcaccatca gcggcaaata agccagcggt ggcaccggac gataaagttc gattaccgga
     3301 tccagaatgc cgcgcaccgt cgggcttagc cccatggcga tcccgaccgg aataccgatc
     3361 accaccgccg cgagtagcgc ctgcacaatg cgcgtcaggc tggctgccag atgctgccac
     3421 agcgtggcgt ccataaatcc ctgcggtccg gcaatggtga gcagttttgc cagtacttgt
     3481 tgcggcggcg gcaaaaacag cgggctaatc agttgcagcg ccgccaccgt ccaccataca
     3541 gtgagtaaaa ccgctaacgt gccaatgctt aaggtcacct gacgcgagag cggccagcgc
     3601 catttcagcc gccgcgaatg cagtttttca ttaatgagca cactcatgag aacgcctccc
     3661 gttgctcaaa tacgcggctt aaaacatatt cgcgcatggc gatgaattgt ggatcggact
     3721 tgatgctgcg gctcgactct cccgcaacaa agcggcgagc aaagttgagc ggcagccgct
     3781 cctgcacacg gccagggccg gatgaaagca gaaccagttc agtcgccata aacaccgctt
     3841 cttctatatc gtgggtaatc aacagcacct gcttgcccgt ctcctgccag agtttcagca
     3901 gcagagtttg catctggtcg cgggtgaagg cgtccagcgc accaaacggt tcatcgagta
     3961 ataacagctg gggattcgcc gccagcgcgc gggcaatccc cacccgctga cgttgaccat
     4021 cggaaagctg ccagatatag cgtttttctg cgccttccag ccccactttt ttcagcatct
     4081 ggtgcgcgat ttccagtcgc tgcattttct ctatacctgc caattgcagg ccgaacgcca
     4141 cgttgtcctg tacattgcgc cacggtagca gcccttcatt ctgaaaaact acgccacgct
     4201 ctgctcccgg tccctcaata cgcttacccg ccagttgaat gctgccatgc tgataaggca
     4261 caaaaccggc aatcagattc agcagggtgg ttttaccgca gccggacggc cccagcacca
     4321 ccagtagctc gccgctttcc agcgtcaggt tgatatcttc cagtgccggt ttgccgccat
     4381 aatcggcata gagatgagag atttgcagca tctgcgcctc cttttattgc acgaagcgcg
     4441 aggtaacgta ctggctgtaa tcattcgcta cagccgggac cttgccctgc tctttcaaaa
     4501 actgcgcggt gtcgatgatt gctttgttca ccggtccggt cagttctgcc gtttgttgct
     4561 gcggcgtcag ataggtattt cccttcacca gccccggaac gtcaccttca ggcacgccgc
     4621 ttaaacgcgc cagtttgctg atgttttccg gctgtttcag ccacgcgtca gggttagcaa
     4681 tgtacggttg ctgtgcatcg atggcgcttt tggcgaacgc tttcacgacc tcaggatgtt
     4741 tctcggcaaa atctttgcgc accacccaga cgtccagcgt tggcgcaccc cactgcccga
     4801 cctgttcaga atcggtcagc accttgccgt ctttttccag ggcgttaacc gccggtgccc
     4861 agacataagc accatcaata tcaccccgct gccatgcggc gataatcgcg ggcggctgca
     4921 ggttcacaat ctccactagc cctggtttaa tcccccagtg tttcagcgcc gccagcaggc
     4981 tgtagtgggt ggtggagata aacggtacgg caatgcgttt accaatcaaa tcttcgggtt
     5041 tgctgatagt tttctttacc accagcgctt cggagttacc cagttttgac gccagcaaga
     5101 agacttcaat cggcac
//
LOCUS       DAJTYG010000181         5105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTYG010000181 DAJTYG010000000
VERSION     DAJTYG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.183"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..390
                     /locus_tag="OO568_003857"
     CDS             <1..390
                     /locus_tag="OO568_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR6642988.1"
                     /translation="MTAGVYLRAFPRHIPAQNAVLDVTFEFPRGRATKDRLYFCVPML
                     DLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLLWYGHCKTVDEA
                     ISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(444..1049)
                     /gene="azoR"
                     /locus_tag="OO568_003858"
     CDS             complement(444..1049)
                     /gene="azoR"
                     /locus_tag="OO568_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6642989.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1250..>5105
                     /gene="hrpA"
                     /locus_tag="OO568_003859"
     CDS             1250..>5105
                     /gene="hrpA"
                     /locus_tag="OO568_003859"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6642990.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRCIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANDGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYP"
BASE COUNT         1263 a         1274 c         1422 g         1146 t
ORIGIN      
        1 atgactgctg gtgtttattt aagggcgttt ccacgacata ttccggcaca gaatgcggtt
       61 ctggacgtca cctttgaatt ccctcgtgga cgagcgacaa aagatcgact ctatttctgt
      121 gtaccgatgt tggatctggt ggttccggaa gagggggagc tccgacaggc cgtggcgatg
      181 ctggaaacat tacgcgaaga gcaaggcagc gttctggtcc attgcgcatt gggattatcg
      241 cgcagtgcgc tggtagtggc ggcatggctg ttatggtacg gacactgtaa aacagttgat
      301 gaagcgatta gctttattcg agccagacgc tcgcatattg tgcttaagga agagcacaaa
      361 gcgatgttga aattatggga aaacaggtaa gtggattgag atgtggactg aatatctaca
      421 gtccacatca agaccgtgtc cggttatgca gcaacaatgc tgtcgatggc tgcttttgcg
      481 tcagactgtg ctttcgctgc catttccgga ccgtatgcga tcccttcggc gaagacaaat
      541 ttcacatcgg taatgccgat aaagccgagg aacgtggaca gatacggcgt caccaggtcc
      601 gttggtccat ctttgtggat cccaccgcgg ctggtaataa cgatggcttt tttacccgtt
      661 accagacctt ccggaccgtt ctcggtatag cggaaagtaa cgcctgcgcg tgcaaccagg
      721 tcaaaatagt ttttcaactg agttgagatg ttgaagttat acatcggtgc cgcaataacg
      781 ataacgtcgt gggctttcag ctcggcaatc agctcatcgg aaagcgccag agcttcctgc
      841 tgacgcggag tcagcggcgc atcgctcgga cgcagagcgc caaccagttc gccatccagt
      901 accggaatcg gatttgcagc taggtcgcga acggtgattt catcagcgga gtgcttttcg
      961 cgccattgtt caacaaaata atcggacaac tgattagact gagagtaccc tgccaggatg
     1021 ctggatttaa gaactaatac cttgctcatg gtgtttcctt atagatgttt gaatggacga
     1081 tgccccgttg cttgctgaca ctttattcac aatcctgcca cagagatagc gcaataaatc
     1141 gaagtctatg ttcgaattta ttgaacaacg catagaaagc cgcgatgtgg tactctatat
     1201 ctatcattta aaagaaaatt aatcaggcag actgctgccc actaacgtta tgacagaaca
     1261 acaaaaattg acctttactg ccttgcaaca gcggctggat tcgctgatgc tgcgcgacag
     1321 actgcgtttt tctcgccgtc tgcattgtgt taagaaggtt aaaaatcctg atgcacaaca
     1381 ggccattttc caggagatgg cgaaagagat tgaccaggcg gcagggaaag tcctgctgcg
     1441 tgaagcggca cgaccggaaa tcacttatcc tgacaattta ccggttagtc agaaaaaaca
     1501 ggacatactc gaagcgattc gtgatcacca ggtggtgatc gtcgccgggg aaaccggctc
     1561 cggtaaaacg actcagctac cgaaaatctg tatggagctg gggcgcggta ttaaagggct
     1621 gatcggtcat acccagccgc gtcgcctggc ggcacgaacg gtggccaacc gtattgcgga
     1681 agagctgaaa acggagccgg gcggttgcat cggttacaaa gtgcgtttca gcgatcacgt
     1741 aagtgataac acgatggtca agctgatgac cgacgggatc ctgctggcgg agatccagca
     1801 agaccgcctg ctgatgcagt acgacaccat cattattgac gaagcgcacg aacgcagcct
     1861 gaatatcgat tttctgctcg gctatttgaa agagttgctg ccgcggcgtc ctgacctaaa
     1921 aatcattatc acttccgcga ctatcgaccc ggaacgcttt tcgcgccact ttaataatgc
     1981 gccgattatt gaagtctccg gtcggaccta tccggtggaa gtgcgctatc gcccgattgt
     2041 tgaagaagcc gatgacaccg agcgcgatca gttgcaggcg atttttgacg ccgtagacga
     2101 actaagccag gaaagccccg gcgacattct gatctttatg agcggcgagc gggaaattcg
     2161 cgataccgcc gatgcgctga acaagctgaa cttacgccat accgaaatct tgccgcttta
     2221 tgcgcggctt tcgaacagcg aacagaaccg cgtgttccag tcgcacagcg gacggtgcat
     2281 tgtgctggcg accaacgtcg cggaaacctc gctgaccgta ccggggatta aatacgtcat
     2341 cgaccccggt acagcgcgta tcagccgcta cagctatcgc accaaagtgc agcgtttgcc
     2401 gattgaaccc atttcgcagg cgtctgccaa tcagcgtaaa ggccgctgtg gtcgtgtgtc
     2461 cgaagggatc tgtattcgtc tctattccga agacgatttc ctctcgcgcc cggagtttac
     2521 cgatccggag attctgcgta ccaacctggc ctcggttatt ttgcagatga ccgcgctggg
     2581 gctgggcgat atcgctgcgt tcccgtttgt cgaagcaccg gataaacgca atatccagga
     2641 tggcgtgcgt ctgctcgaag aactgggcgc gatcaccacg gatgaacagg ccagcgccta
     2701 taaactgacg ccgctcggtc gccagctctc gcagttgcct gtcgacccac gtctggcgcg
     2761 tatggtgctg gaagcgcaaa aacatggctg cgtgcgtgag gcgatgatta tcacgtccgc
     2821 gctctccatc caggatccgc gcgaacgtcc gatggacaaa cagcaggcat cggacgaaaa
     2881 acatcgtcgc ttccacgaca aagaatccga cttcctcgcg tttgtaaatc tgtggaatta
     2941 tcttggcgag cagcaaaagg cgctttcttc caacgccttc cgtcgcctgt gtcgtaccga
     3001 ttacctcaac tatctgcgcg tgcgcgaatg gcaggatatc tacacccagt tgcgccaggt
     3061 ggtgaaagaa cttggcattc cggtcaacag cgaaccggcg gagtatcgtg aaattcacat
     3121 cgccttactg accggtttgc tttcccatat cggcatgaaa gatgccgata aacaggaata
     3181 taccggtgca cgtaacgcgc gtttctccat cttccccggt tctggtttat tcaaaaagcc
     3241 gccgaaatgg gtaatggtgg cggaactggt agaaaccagc cgcctgtggg ggcgcattgc
     3301 tgcgcgtatc gacccggaat gggtagaacc cgttgctcag catttgatta aacgcaccta
     3361 cagcgaaccg cactgggaac gggcgcaggg cgcggtgatg gcaacggaaa aagtcactgt
     3421 ttatggtttg ccgattgttg ctgcgcgcaa ggtcaactac agccagatcg attcggcgtt
     3481 atgtcgtgaa ctctttattc gccacgctct ggtggaaggt gactggcaga cgcgtcacgc
     3541 attcttccgt gaaaacctga aactgcgggc cgaagtggaa gagctggaac acaaatcacg
     3601 tcgccgcgat attctggttg atgacgaaac gctgtttgag ttctacgacc agcgcatcag
     3661 ccacgatgta atctccgctc gtcacttcga tagctggtgg aaaaaagtca gccgcgaaac
     3721 gcctgatttg ctcaactttg aaaagagcat gttgatcaaa gaaggggcgg aaaaaatcag
     3781 caagctggat tacccgaact tctggcatca gggcaatctc aagctgcgtt tgagctatca
     3841 gtttgagcca ggcgcggacg cggacggtgt gaccgtacat attccgctgc cgttacttaa
     3901 ccaggttgag gaaagcgggt ttgaatggca gatccccggt ctgcgccgcg aattggtgat
     3961 tgctctgatt aaatcgttgc cgaaaccggt acgccgtaat tttgtgcctg caccaaacta
     4021 cgccgaagcg tttttaggcc gcgtcacacc gctggagtta ccgttgctcg acagccttga
     4081 gcgcgagtta cggcggatga ccggcgttac cgttgaccgc gaagactggc actgggatca
     4141 ggtgcccgat cacctgaaaa tcaccttccg cgtggtggat gacaaaaaca agaagctaaa
     4201 agaagggcgc tcgctacaag atctgaaaga tgcgctgaaa ggcaaagtgc aggaaacgct
     4261 gtccgcggtg gcggatgacg gtatcgagca gagcggctta catatctgga gttttggtca
     4321 gctgccggaa agttacgaac agaagcgtgg caactataaa gtgaaggcgt ggccggcgct
     4381 ggtggatgaa cgcgacagcg tggcgatcaa actgtttgat aacccgctgg agcaaaaaca
     4441 ggcaatgtgg aatggtcttc gccgtctact gctgctaaat atttcctcgc cgatcaagta
     4501 tttacatgag aagttaccaa acaaagccaa gctggggctg tactttaacc cgtatggcaa
     4561 agtgctggag ctgatcgacg actgtatctc ctgcggtgtg gatcaactga tcgacgccaa
     4621 tgatggcccg gtctggacgg aagaaggctt tgctgcgctg catgaaaaag tgcgcgccga
     4681 actgaatgac acggtggtgg atattgcgaa gcaggtcgag caaatcctca ctgcggtgtt
     4741 caatatcaac aaacgtctga aagggcgggt ggatatgacc atggcactgg ggctttctga
     4801 cattaaagcg cagatgggcg ggttggtata tcgcggtttt gtcactggta acggcttcaa
     4861 acggctgggc gacacgctgc gatatttgca ggcgattgaa aaacgactgg aaaaactggc
     4921 ggttgatcca catcgcgatc gtgcacagat gctgaaagtc gaaaacgtcc agcaggcgtg
     4981 gcagcaatgg atcaacaaac tgccgcctgc acgtcgcgag gatgaagacg tgaaagagat
     5041 ccgttggatg atagaagagc tgcgcgttag ttacttcgct caacaacttg gtacgcctta
     5101 tccga
//
LOCUS       DAJTYG010000182         5009 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTYG010000182 DAJTYG010000000
VERSION     DAJTYG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.184"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..107
                     /locus_tag="OO568_003860"
     CDS             <1..107
                     /locus_tag="OO568_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="with Hns involved in transcriptional regulation of
                     hemolysin; non-specific DNA-binding protein which affects
                     the production of multiple proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6642991.1"
                     /translation="ERESFNSAADHRLAELITGKLYDRIPKEIWKYVR"
     gene            146..736
                     /locus_tag="OO568_003861"
     CDS             146..736
                     /locus_tag="OO568_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6642992.1"
                     /translation="MKLIIFILIVLIIATLLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            976..1236
                     /gene="repA"
                     /locus_tag="OO568_003862"
     CDS             976..1236
                     /gene="repA"
                     /locus_tag="OO568_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR6642993.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1460..1534
                     /gene="tap"
                     /locus_tag="OO568_003863"
     CDS             1460..1534
                     /gene="tap"
                     /locus_tag="OO568_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR6642994.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1527..2384
                     /gene="repA"
                     /locus_tag="OO568_003864"
     CDS             1527..2384
                     /gene="repA"
                     /locus_tag="OO568_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6642995.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3084..3275
                     /locus_tag="OO568_003865"
     CDS             3084..3275
                     /locus_tag="OO568_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642996.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
     gene            complement(3505..4692)
                     /locus_tag="OO568_003866"
                     /pseudo
     CDS             complement(3505..4692)
                     /locus_tag="OO568_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4692..>5009)
                     /locus_tag="OO568_003867"
     CDS             complement(4692..>5009)
                     /locus_tag="OO568_003867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6642997.1"
                     /translation="SKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCADLNIPFF
                     VDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMYCPECGTVHWPDGVA
                     PPF"
BASE COUNT         1280 a         1335 c         1231 g         1163 t
ORIGIN      
        1 gtgaacgtga atcatttaat tcagcagcag accaccggct agcggaatta atcaccggaa
       61 agttatatga caggatacca aaagagatat ggaaatatgt aaggtaataa ttcagcaata
      121 attaattgcc aggagcagat aaataatgaa gttgattatt tttattctca ttgttctgat
      181 tattgcaacg ctacttatcc ggattatcct cagaagtgta aatcagcatt ctcctttgct
      241 gatgcaattg catgcggcag gtatacggac tggtgatgca gagcgaatac tgtccagcgg
      301 tgaatactgg caacgtcaga agacgctgct gacagaaagg gaagtcagtt ttatgaaagg
      361 actgttcaga attgtggata tgaagcggtg gtatctgtgt ccgcaggtac gggtcgcgga
      421 tatcgtccag ctgaacggga atatccggcc acgatcacgc cagtggtggc agttattcag
      481 gatggtgtct cagtggcatg ttgatgtggt tatcgttgag ctgcgttcgt tcagtattgt
      541 tgcagcagta gagctggatg atgccagcca tttacgaccg gaacgcagac gccgggatat
      601 tcttctggaa gaggttctga ggcaggctgg tattccgttg ctcagaagcc acgatgccag
      661 aaaactgctg cagatgaccg gagaatggct gaatacaaca ggggctgatc agcagtcacc
      721 ggaacatcgt agctgacgcc ttcgcgttgc tcagttgtcc aaccccggaa acgggaaaaa
      781 gcaagttttc ctcgctcccg gcgtttccat aactgaaaac catactattt cacagtttaa
      841 atcacattaa acgacagtaa tccccgttga tttgtgcgcc aacacagatc ttcgccacaa
      901 ttctcaagtc gctgatttca aaaaactgta gtatcctctg cgaaacgatc cctgtttgag
      961 tattgaggag gcgagatgtc gcagacagaa aatgcagtga cttcctcatt gagtcaaaag
     1021 cggtttgtgc gcagaggtaa gcctatgact gactctgaga aacaaatggc cgctgttgca
     1081 agaaaacgtc ttacacacaa agagataaaa gtttttgtca aaaatcctct gaaagatctc
     1141 atggttgagt actgcgagag agaggggata acacaggctc agttcgttga gaaaatcatc
     1201 aaagatgaac tgcagagact ggatatacta aagtaaagac tttactttgt ggcgtagcat
     1261 gctagattac tgatcgttta aggaatttta tggctggcca cgccataagg tggcagggaa
     1321 ctggttctga tgtggatgta caggagccag aaaagcaaaa accccgataa tcttctttaa
     1381 ctttggcgag tcagaaagat taccggggcc cacttaaacc gtatagccaa caatcaagct
     1441 atgcggggag tatagttata tgcccggaaa agttcaagac ttctttctgt gctcgctcct
     1501 tctgcgcatt gtaagtgcag gatggtgtga ctgatcttca acaaacttat taccgccagg
     1561 taaagaaccc gaatccggtg ttcacacccc gtgaaggtgc ccggacgctt ccgttctgcg
     1621 ggaaactgat ggaaaaggcg gtgggcttca cctcccgttt tgatttcgcc attcatgtgg
     1681 cacatgcccg ttcgcttggt ctgcgtcggc gcatgccacc ggtactacgt cgccgggcca
     1741 ttgatgcgct gctgcagggg ctgtgttttc actatgaccc actggccaac cgcgtccagt
     1801 gttccatcac cacgctggcc attgagtgcg gactggcgac ggagtctgct gccggaaaac
     1861 tctccatcac ccgggccacc cgggccctga cgttccttgc agagctggga ctgattacct
     1921 accagacgga atatgacccg cttatcgggt gctacattcc gaccgatatc acgttcacat
     1981 ctgcactgtt tgctgccctc gatgtatcag aggaggcagt ggccgccgcg cgccgcagtc
     2041 gtgtggaatg ggaaaacaga cagcgcaaaa agcaggggct ggataccctg ggtatggatg
     2101 aactgatggc gaaagcctgg cgttttgttc gtgagcgttt ccgcagttac cagacagagc
     2161 ttaagtcccg tggaatgaag cgtgcccgtg cgcgtcgtga tgcggacagg cagcgtcagg
     2221 atattgtcac cctggtgaaa cggcaactga cgcgtgaaat ctcggaaggg cgcttcactg
     2281 ccagtcgtga ggcggtaaaa cgtgaagtgg agcgtcgtgt gaaggagcgc atgattctgt
     2341 cacgtaaccg taattacagc cggctggcca cagcttcccc ctgaaagtga cctcctcaga
     2401 ataatccggc acgcgccgga ggcattcgca cgcctgaagc ccgccagtgc acaaaaaatc
     2461 agcgctacat acaaaaaaca acctcatcat ccaccttctg gtgcatccgg tcccccctgt
     2521 ttttgataca aaacacgccc cacagacggg gaattttgct tatccacatt aaactgcaag
     2581 ggacttcacc gtaaggttac aaccgttcac gtcataaagc gccagccgtc aaccctacag
     2641 ggtgcaatgt atcttttaaa cacctgttta tatctccttt aaactactta attacattca
     2701 tttaaaaaga aaacctgttc actgcctgtc ctgtggacag acagatatgc acctcccacc
     2761 gcaagcggca ggccccaacc gaagccggtt tagttacaac atgcaaacat aaacccctaa
     2821 aaaacccaag tccaacgcag aactgaaacc acaacgcccc tcctcataac tgaaaagcgc
     2881 cgccgccccg ccctaagggc gggaacagag tcgcttttaa ttatgaatgt tgtaactaca
     2941 tcatcatcgc tgtcagtctt ctgcctggac gtatcgagta cacgctcgta agcggccctc
     3001 acggcccgct aacgcggaga tacgccccga cttcgggtaa aaccctcgtc gggaccactc
     3061 cgaccgcgca cagaagctct atcatggctg aaaacgggta tggcttagca gggctgggga
     3121 tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc gggcttcggc ggttttactc
     3181 cggtatcaca tgtaaccaca gcgtgtcgcc ttccatgccg ctggcgcggc atcagaataa
     3241 tacaagcagc atttaatgca tttgcaaaag tgtgagtaat attaagtggt cgtttcaggg
     3301 atatcgttaa gacttgagcg ataggaatta aaaccccaaa aagattaaaa aaacaccaca
     3361 aaacggatgt ttcttcaaca ccacttttgc tacatatgaa cggaaccgac gattaaactg
     3421 gatggctctg attgattcag ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg
     3481 gacgcaactt cccctctgcg gctctcagcc gcaccaccgc atccgggcca gcggctcatg
     3541 catcaggacc tgctctgcca gacggtagcc ccgcttcagc cccgtaaaac gcatctgact
     3601 cccgcacagc acgcacttca gcgggtcaac cttcagtaac ctctgataca tccctctcca
     3661 ggtgatttgc atcgccgttt ttctcactgt ctccgttatg atgtacacca cttcttccag
     3721 taaccgccgt ttcgccggac tcaaaaaacc gtagtacctc accatacgga accccttatc
     3781 cgccacatgc caggagaacc tttccatgaa ctcatctcca ctcatcaaca ggtattcttc
     3841 ccgttttgtt cggtgactgt tgtaacgcag accgatttca tcctgaccgg cataatgctc
     3901 cagacgactc atcggcaccg gtggcttttt caggtaagag ccaaagtaca ccgccacatg
     3961 ggtggcatta tccatcaccc gggatacgtt gacatttcag ccacggcggt aatgcgtgtc
     4021 caggaagcga ttccattccc gtttactgct tccttctgct gccagcgcat ccggcatcac
     4081 caggtcaggg tatttccgtg acagcaaccg tgttatccgg tagcgccaca tgctcatcac
     4141 cttacgggcg taaaaatgaa gatttttcca ggtgtggccc gacgtcacac caccggcagt
     4201 tgtcgataaa tggatatgcg gatgccactg ctggtcacgc ccccatgtgt ggctcaccgt
     4261 gaatatcccc ggctccacat ctgcctgatg gcagatttcc agtatcacat ccgctgcaat
     4321 gcggctcatc tctgccagta accaccggtt gtggaacacc agggaccagt actggcaggg
     4381 aagtgtgaac acaatatgct gccacgggca gtcggggacc aggctcagca gatactgtat
     4441 ccactgtgcg ccagccttca ccccgcagtg cgggcaggag cggcttttac accggaagca
     4501 gacctttttt gtatggcaac agtccggtga tgaacaacac cactgcgtgt agcccatcag
     4561 tgtggtcccg caggccatga tttttgtcac tgattcaatt accaccggac ggactgcccc
     4621 ttccggttgt ttctccagcc agttaagcca gcggtttccc tgctgaaaaa tatcggcaaa
     4681 acggggaagc atcagaaggg cggggcgact ccgtccggcc agtgaaccgt gccacactcc
     4741 gggcagtaca taccgccggc gctgataccg gcaagaatgg tcgcaaattc ccgctccgtg
     4801 cagcgggcga tttccggata cccttcgtca tcaacacgta caaaccagaa gaccagcttt
     4861 ttgtttcccg catccacaaa gaacggaata ttcaggtctg cgcagcattc aacggcatcg
     4921 tcaaaactat caaagcgcag aacttctgcg tcttcttcgt caaaaaaatc atcttcgtga
     4981 agcttcacga catagcgggg aagtttgct
//
LOCUS       DAJTYG010000183         5001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTYG010000183 DAJTYG010000000
VERSION     DAJTYG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.185"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            190..447
                     /locus_tag="OO568_003868"
                     /pseudo
     CDS             190..447
                     /locus_tag="OO568_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO568_003869"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO568_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6642998.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO568_003870"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO568_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6642999.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO568_003871"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO568_003871"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6643000.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNLISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="OO568_003872"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="OO568_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3605..4420)
                     /locus_tag="OO568_003873"
     CDS             complement(3605..4420)
                     /locus_tag="OO568_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6643001.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            4517..>5001
                     /locus_tag="OO568_003874"
     CDS             4517..>5001
                     /locus_tag="OO568_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6643002.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     KV"
BASE COUNT         1332 a         1140 c         1182 g         1347 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagatc atcaatagtc agggaaggaa gttgcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt tcaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgagat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aatcatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagaagg t
//
LOCUS       DAJTYG010000184         4951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTYG010000184 DAJTYG010000000
VERSION     DAJTYG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.186"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..610
                     /locus_tag="OO568_003875"
                     /pseudo
     CDS             1..610
                     /locus_tag="OO568_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
     gene            631..1291
                     /gene="csiR"
                     /locus_tag="OO568_003876"
                     /pseudo
     CDS             631..1291
                     /gene="csiR"
                     /locus_tag="OO568_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
     gene            complement(1292..1741)
                     /gene="kbp"
                     /locus_tag="OO568_003877"
     CDS             complement(1292..1741)
                     /gene="kbp"
                     /locus_tag="OO568_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6643003.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(1825..1983)
                     /locus_tag="OO568_003878"
     CDS             complement(1825..1983)
                     /locus_tag="OO568_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6643004.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            2166..2465
                     /locus_tag="OO568_003879"
     CDS             2166..2465
                     /locus_tag="OO568_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6643005.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            2475..2999
                     /gene="ygaP"
                     /locus_tag="OO568_003880"
     CDS             2475..2999
                     /gene="ygaP"
                     /locus_tag="OO568_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6643006.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKIPWNQRA"
     gene            complement(3046..3450)
                     /gene="stpA"
                     /locus_tag="OO568_003881"
     CDS             complement(3046..3450)
                     /gene="stpA"
                     /locus_tag="OO568_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6643007.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            4118..4555
                     /gene="alaE"
                     /locus_tag="OO568_003882"
     CDS             4118..4555
                     /gene="alaE"
                     /locus_tag="OO568_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6643008.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKGWIKNLADILAYVTFQSPVYVAILLVVGAD
                     WHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(4592..4942)
                     /locus_tag="OO568_003883"
     CDS             complement(4592..4942)
                     /locus_tag="OO568_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6643009.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERQRHEHYGIPHGFSSRV
                     ALERYLNGLFGEAS"
BASE COUNT         1259 a         1170 c         1166 g         1356 t
ORIGIN      
        1 gccggggctg aaagccgttg gatcttatcg ctcggttctg gaattgctca atattcccca
       61 tgcgaaatta atcatggact gtgtgatatt actttccgta accagttgcc tgaactcggc
      121 cctgtatacc gcctcaagga tgctctactc cttaagccgt cgcggtgatg ctcccgcggt
      181 aatgggtaaa atcaaccgca gtaaaacccc gtatgtggcg gtgttactct ccaccggcgc
      241 agcattctta acggtggtgg tgaactatta cgcgcctgcg aaggtattta aatttctgat
      301 cgacagctcc ggcgctatcg ccctgctggt ttatttagtc atcgccgttt cacagttgcg
      361 gatgcgcaaa attctgcgag cagaaggaag cgaaattcgc ttgcgaatgt ggctttatcc
      421 ctggctcacc tagctggtca tcggctttat tacctttgtg ttggtagtga tgctattccg
      481 tccggcgcaa cagttagaag tgatctccac cggcttatta gcgataggga ttatctgtac
      541 cgtgccaatt atggctcgct ggaaaaagct ggtattgtgg caaaaaacac ccgttcataa
      601 tacgcgctga taatgatcag gagtcacacc atgaccatta cgtctctgga tggctatcgc
      661 tggctgaaga acgatattat tcgcggtaat tttcaaccgg atgaaaaatt acggatgagt
      721 ttactgacat cgcgttatgc acttggcgtt gggccgttgc gggaagtact ttctcaactg
      781 gtggcggaac ggctggtcac ggtggtgaat caaaaagggt atcgggtggc gtctatgtca
      841 gagcaggagc tgctctgaca tagacgcccg cgccaatatg gaagcgatgc tggtcagtct
      901 ggcgattgcc cgcggtggcg atgaatggga ggcagacgtt ctcgcaaaag cgcatctgct
      961 caataagctg gaagcctgtg acgccagcga gaaaatgctt gatgagtggg atctgcgtca
     1021 tcaggcgttt catacagcaa ttgtggcggg ctgtggttct cactatttgc tgcaaatgcg
     1081 tgaacggttg tttgatctgg cggcgcgtta tcgatttatc tggctgcggc gaacggtgct
     1141 ttcggtggaa atgctggagg ataaacacga tcagcaccag accctgactg cggcggtact
     1201 ggcgcgagat acagcgcgcg ccagtgagtt aatgcgccag catttactga cgccaattcc
     1261 cattatccag caggcgatgg ctggcaatta actactcttc cggaatacgc aacacctgcc
     1321 ccggataaat tttatccggg ctttttagca tcggtttatt cgcttcgaag attttattgt
     1381 acagattagc gttaccgtag acctgtttgg aaatggcact cagagtgtcg ccagacttaa
     1441 cggtataaaa ctggctggca gtggctggtg tcgccgtttt cacctgatca tcaacactgg
     1501 caataccgga aatattcccc accgcaacaa ggattttctc cttcgcctcc tgactcaggc
     1561 cgtcaccagt gaccgtcgct ttgccgtcgg caatttgaat gttcacttta tcggcatcag
     1621 gtataccggt tttgctcaga tgctcctgca ccttcttcgc ctgatcgtct ttatcgtgct
     1681 gacctgtaac cgcatcccag agtttttctc cggcatcttt cacaaaattg aacagaccca
     1741 tagctacctc attcgttaac ggaaacactc agaaagtgta gcagaggaac gcggcgcgtt
     1801 aacgccgcgg gggtgagcac aggtttaatc gcgggtttgc acccagaacg cgtgaatcaa
     1861 accggggata tacccaagca gtgtcagcag aatattaata atgaacgccc aaccgaaccc
     1921 tttaccgagc agcacgccga gcggcggcag aatgatggtg atgacgattc tccagaaacc
     1981 catatgtact ccctataaga aaattactca ttgtttaaaa agagacttta tctcttaagc
     2041 gtagtaattt tagcggagcc tgccagtttc agcttacgct tttactctca tttacgctgc
     2101 tgaggctggt atatgttgca tttttagttt agagtttact taatttagaa aatacttaaa
     2161 taaatatgac tgaactcgcg caattacagg ccagtaccga acaggcagcg gccttattga
     2221 aagcaatgag ccaccctaaa cggttgctga ttctgtgcat gcttagcggt tcccccggca
     2281 ccagcgcggg agagctgacg cgcattaccg gactgagcgc ctctgcgaca tcacagcatc
     2341 tcgctcgtat gcgggacgaa gggcttatca acagccaacg ggatgcccaa cgcattctat
     2401 attccattaa aaatgaggcg gtaaatgcca ttatcgccac cctgaaaaat gtctattgtc
     2461 cgtaaggagt cactatggct ttgacaacca tttcgccgca tgatgcacaa gaattaatcg
     2521 cacgcggcgc aaagttaatc gatattcgtg acgctgatga atatcttcgt gaacatattc
     2581 ctgaagcaga tctggctcca ttatccgtgc tggaacagtc aggtcttcca cctaaattac
     2641 gtcgcgagca aattatattc cactgccagg caggtaaacg caccagtaat aacgccgata
     2701 aattagcagc gattgccgcc cccgcagaaa tatttttact cgaagatggg attgatggct
     2761 ggaaaagagc tggattgcca gtagcggtaa ataaatctca acccttgccg ttaatgcgcc
     2821 aggtgcagat cgctgcgggt ggtttaatat taatcggcgt tgtactgggt tataccgtaa
     2881 atagcggttt cttcttatta agtggctttg ttggtgccgg gttactgttt gcaggaatca
     2941 gcggtttttg tggaatggca aggttgttag ataagatacc gtggaaccaa cgagcttgag
     3001 aagcgacgcc ggacgcgccc tagcagcgac atccggcctc agtaattaga tcaggaaatc
     3061 gtcgagagat ttaccttctg ccagcgcctg agcaattggc ttcggtgtac ggccctgacc
     3121 ggtccaggtt ttagtttcac cgttaacatc agtgaattta tatttcgccg gacgcggctg
     3181 gcgtttttta ccagcacgtg gtgcagcagc agagctatta cccaataact cttccgggtt
     3241 aattccgtca gctttcatca gctccagcca ggtgctaatt ttttcctgac gctcagccag
     3301 ttcacgctgc tgctgttctt cttcttcacg tctttcttta gtgacaaccc tgaatttttc
     3361 gagcatttct tcaagaacgt caatggagaa ttcgcgagcc atcgcacgga gggtgcgaat
     3421 gttatttaaa ctttgtaaca ttacggacat aataaagaaa accttttaac gccaaaacaa
     3481 aaaagtattt cgtcgcgtag tgtaagcgat cacttattat atttccacaa aatttaatct
     3541 atttacagtc ctgcgcgaga ttatttcagc tgttattttc tgaaactctc agaataattt
     3601 caagattata tattagccat tttgatgagt aatttaggtt attggtgaac agagtggcgc
     3661 tctctgctcg ctaactccgt aagcctgaca aaaccgaagt gaataaaaat gcaatataac
     3721 agattaaaag aaaacacccc tcactggtgc tttctgctgt taatgtacat tttctggcat
     3781 caaaaatgct gtaaatgacc cggtcacgac aaacaccgaa ttctctacgc ttatagcgtt
     3841 acctcccccc caaactttca ttagtgaaat taaaggcaag gccatatcca attgatttaa
     3901 ttgaaaataa atcatatcga catgtaaaaa tccacagtgt acaaaaaacg agctgttacg
     3961 gaatatttta tctacaaaaa ctgactaaat aaaaattttt cactaattga ttagtcatag
     4021 ccagcgatat acgctatgcg aaaatacaga tggcaatgag atccactgct ttcatctcca
     4081 ttaacatccc attacgcttt tattaaggag cattagcatg ttctcaccgc agtcacgctt
     4141 gcgtcatgca gttgcagata cgttcgcgat ggttgtttac tgttctgtcg tgaacatgtg
     4201 tattgaagtt ttcctctccg gaatgagctt cgaacagtct ttttattcca gattggtagc
     4261 gattccggtg aacatcttaa ttgcatggcc atacggtatg taccgcgatc tgtttatgcg
     4321 cgcggcacgc aaaggctgga taaaaaatct ggctgatatc ctggcttatg tgacgttcca
     4381 gtcaccggtg tatgtggcga tcttgttagt ggtgggcgca gactggcatc agattatggc
     4441 ggcggtcagt tcaaacatcg ttgtttcgat gttgatgggg gcggtttatg gctacttcct
     4501 cgattattgc cgccgactgt ttaaagtcag ccgttaccag caggtaaaag cctgactctt
     4561 cttttcgcga ctggcatcgc cagtcgcgtc tttaactggc ttcgccaaat aaaccattca
     4621 aataacgttc aagcgcaaca cgcgaactaa agccatgcgg gataccgtaa tgctcgtgtc
     4681 gctgtcgctc gcctgctaaa tagagcggaa attgctggta atgatcgcaa gttttaatat
     4741 cggaagcggg ttcagccagt gttgaactgc gttcttttaa tgttggatga attaccagag
     4801 cggtacgtcc cattcgcgct tcgcgattaa catagacata attttcccca cggcggtaac
     4861 catacgcttt ctgggttacc acatcgacag taaaaccgac tttttcaagt acgcgcgcca
     4921 cctcgtctgg tcgtaaatac atatttgatc c
//
LOCUS       DAJTYG010000185         4823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTYG010000185 DAJTYG010000000
VERSION     DAJTYG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.187"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            83..198
                     /gene="rrf"
                     /locus_tag="OO568_003884"
     rRNA            83..198
                     /gene="rrf"
                     /locus_tag="OO568_003884"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            213..288
                     /locus_tag="OO568_003885"
     tRNA            213..288
                     /locus_tag="OO568_003885"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:246..248,aa:Thr,seq:ggt)
     gene            328..443
                     /gene="rrf"
                     /locus_tag="OO568_003886"
     rRNA            328..443
                     /gene="rrf"
                     /locus_tag="OO568_003886"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(506..1264)
                     /locus_tag="OO568_003887"
     CDS             complement(506..1264)
                     /locus_tag="OO568_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6643010.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQYFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1272..2375)
                     /locus_tag="OO568_003888"
     CDS             complement(1272..2375)
                     /locus_tag="OO568_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6643011.1"
                     /translation="MTKVFLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLILASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2385..3555)
                     /locus_tag="OO568_003889"
                     /pseudo
     CDS             complement(2385..3555)
                     /locus_tag="OO568_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3623..4648)
                     /locus_tag="OO568_003890"
     CDS             complement(3623..4648)
                     /locus_tag="OO568_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6643012.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1324 a         1251 c         1158 g         1090 t
ORIGIN      
        1 tgttttggcg gattgagaga agattttcag cctgatacag attaaatcag aacgcagaag
       61 cggtctgata aaacagaatt agcctggcgg ccgtagcgcg gtggtcccac ctgaccccat
      121 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
      181 agtagggaac tgccaggcat caaatttagc gtgctgatat ggctcagttg gtagagcgca
      241 cccttggtaa gggtgaggtc cccagttcga ctctgggtat cagcaccact ttttaggtta
      301 aagttcggca gattagaaaa gaatttgtct ggcggcagta gcgcggtggt cccacctgac
      361 cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat
      421 gcgagagtag ggaactgcca gacatcaaat caagcgaaag gccatccgaa aggatggcct
      481 ttttgctttt cgaactaaca ttcaattaat ggattacctg cgataaaaat gccctcgtac
      541 gctctgattt aggatgcgca aaaaattcat caggtgtagc ttgttccact atctccccac
      601 gatccataaa aattacccgg tcagcgacgg tacgtgcaaa ccccatctca tgtgttacac
      661 acaacattgt catacccgac tgcgccagcc caatcatcgt atccagcacc tctttcacca
      721 tctccggatc gagcgccgac gttggttcat caaacaacat aattttcggc ttcatacaca
      781 gcgaacgcgc aatggcaacg cgttgctgct gaccacctga aatctgtccg ggaaacttat
      841 gcgcatgttc ggcaattctc acccgctcaa ggtaatgcat cgccagagct tcagcctctt
      901 tcttaggcat cttgcgtacc caaatcggtg ccagggtaca gttctgtaaa acggtcagat
      961 gagggaagag attgaaatac tgaaatacca ttcccacttc ctgcctgacg cgctcaatat
     1021 tgcggatatc ttcattaagt tcgatgccat ctacgacaat ccgtccctgt tgatgttctt
     1081 ccagatgatt aatacaacga atggttgtcg atttaccgga accagaaggg ccacacagaa
     1141 cgatccgttc tcccggttgc acggttaaat ttatattttt caaaacatgg aattgtccat
     1201 accatttatg gacgttttcc agcgtaatca tcgcgttagc aggttgcagt aaaatttggc
     1261 tcatagtgtc ctcaatgcgg tgtacgcccg gtgttaaaac gtttttccag atgttggcta
     1321 taacgtgaca tgctgaaaca aaagatccag tagatcagcg cggcgaaaac atacccttcc
     1381 gtcgacatac ccaaccaggc gggatcaacg gttgcctgct gaacgctact gaaaagatcg
     1441 aacaagccga tgatgatcac taggctggta tctttgaaga gtgcgatgat ggtatttacc
     1501 agcccaggaa ttaccagctt caacgcctgt ggcagaataa ccagcccctg agttttccag
     1561 taacccaacg ccagcgactc tgccgcttca tattgccctt taggcagcgc ctgtaatcca
     1621 cctcgcacga cttccgcaac atatgctgac tgaaacagga tcacgccaac cagcgcgcgg
     1681 atcagtttgt cgatactggt gccttctgcc ataaacaacg gcagcatgac cgaagacata
     1741 aacaatacgg taatcagcgg tacgccgcgc cagaactcga taaaaatgac cgataagata
     1801 cgcacaatcg gcatatggga gcggcgacct aatgccagta agatccccca cggtagcgcc
     1861 cccgcaatcc caacggatgc aagaattaaa gttagcgtca gcccgcccca ttgccgggtt
     1921 tcaacccgct caagaccaaa aaaaccgcca tacatcagcc accagacaat cagtgggtaa
     1981 atcaccgccc aggccgcaat atagcgaccg cgatgcggga gtattttcca gaacattggt
     2041 gcgatcgata caagcccaat cagtaatgcg aggttaattc gccagcgttg gtcgtgtggg
     2101 taaagcccat acataaactg accaaatcgt tcgtgaatga agacccaaca ggcaccggct
     2161 tttgtgcagt cagcacgcgt tgagccaacc caattagcct gcaaaaatgc ccagttcagc
     2221 aacggtggga tcagttccca catcaaccat atgcagccaa tagtcagcag gctattgctc
     2281 cagctggaga acagattttt tcgcacccac accatcgcgc ggcttgagtt atggctcgcc
     2341 gggcgcgagg gctgagacag gaatactttt gtcatcatga ctccttagcg ttcaacgatc
     2401 gcgatgcggc ggttatagat attcatcagc agcgagatag tcaggctgat aatcagatag
     2461 accgacatgg tcatggctat cgtctcgatg gcttgccccg tctggttcag cacggtgcca
     2521 gcaaacagcg aaaccatatc gggatagcca atagcggcgg caagagagga gtttttgacg
     2581 atgttgagat actggctggt taacggcgga ataattactc gcagtgcctg gggaataatg
     2641 acctggcgta gcgtaaccgg gttgggtaac cccagtgacc gagccgcttc atgttgacca
     2701 taaggcactg cctggatccc agcgcggata atctcggcga taaatgcaga ggtataaacc
     2761 gaaagtgcca gcgttaaggc tgccagttca ggaattaaaa ccattccgcc gcggaagtta
     2821 aagccttgta gggctgggac atcccagtgt agtgctgcgc caaaaagcca ttgcgccagt
     2881 aaaggcaaac caatgatcaa cactgcggcg atcggccagg agcggcgcag ttgtccggtc
     2941 tttatctggt atgttttatt aaagcggaat agcccaacag aaaggactat agccataaca
     3001 acagccagga taaacgcaat aaatccgtct cccagctgcg gtgacggaat ataaagccca
     3061 cgattgctca aaaaggccag atcaaacgcg ctcacagctt ggcgtggtcc gggcaaattg
     3121 cgcaacacgg caaagtacca gaagaagatt tgcaatatta cggaagatct caatataaat
     3181 tgtggaaagc tttcgtagca gccagttatc cgaaagtctc gccagaccga taaagaagcc
     3241 caaaacagaa gcgaacacaa tacacaatgc agaaaccagt agcgtattga gtaagccgac
     3301 aataaaaacg cgtccgtagg tgtcgccctg ctggtaatcg atcaaatgct ggacaatacc
     3361 gaagccagcg ccgcgatcca gaaaggcaaa acctgaagta atgccacgat tattgagatt
     3421 cgttacagtg ttttgaaata accaaccaac aatgccgaca acagcaacaa cggcaaggat
     3481 ctgaaataac caggcacgaa ccgtagggtt ggcaaaggag agtgagcctt taacggttga
     3541 gcggcgatga gacatagaaa cctcggaaag cataacgctg aagagagcgc cgccgtagcg
     3601 acgctcgtta cctcacaccg acttaacgca cgggcggcgc gtactgaata ccgccgttat
     3661 tccagagatt attttgccca cgtttaattt tcagcgggct ttctgaacct acgttacgct
     3721 caaaaatttc cgagtagtta cccacctgtt tgatgatgtt ataggcccat ttattatcca
     3781 acttcagatc cttgccgtaa tcgccttctt tacccaacag atgtgccata tcaggcgttg
     3841 ctggattagc cgctttttca tcgacgttct gggaattgat gcccatctct tcagcattta
     3901 gcatggcgaa aagcgtccag cgtacaatcg agaaccattc atcatcgcca cgacgaacta
     3961 ccggcccaag cggttcttta gagataacct ccggtaagac aatccattca gccgggttgc
     4021 ttaatttgat gcgcaaggca tacagttgcg actgatccga agccagcgta tcgcagcgac
     4081 cagattccag tgccttcgct gattcgtcag agcgatcgaa agtcactggt gtgtacttca
     4141 tattgtttgc cttgaaatag tcggcgacgt tgagttcggt atcagtaccc gcctgaatac
     4201 agacggtagc gccatccagt tctttcgcgc tttttagccc cgctttatcg tgcgtcagga
     4261 agccaatgcc gtcgtaataa gtgacgccgg taaatgccat tcccatcccg gcatcgcgag
     4321 atgaagtcca ggtcgtatta cgggagagca aatccacttc ccctgactgt aaagcggtga
     4381 agcgttcttt tgctgtgagt ggggtatatt tcactttcgt gtcgtcacca aatacagcag
     4441 cggcaacacc acgacaaaca tcaacatcaa tacctgaaaa cttaccgtct gcatcggcat
     4501 aagagaacct aggtaatcca tcactgatcc cgcattgcac aaaacctttt ttctgaacgg
     4561 catcaagcgt cgcgccagca tgcgcctgat ttgcaacggc aagcagcacg ctggcggcag
     4621 ccagtgtggc tatcatcatc tttttcataa tgcttcctgt gaggcgaaaa ttatcgttat
     4681 aggacgtttg ggaaacgtct tttacctgtc tgtggccatc ctcttataag caaaagtagt
     4741 gccagctttt gcgcacgatt tcgcgtcgca ataagctgag tgtagacagg aaataataaa
     4801 aacagcgccc cgcaatggtg cga
//
LOCUS       DAJTYG010000186         4541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTYG010000186 DAJTYG010000000
VERSION     DAJTYG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.188"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            124..567
                     /locus_tag="OO568_003891"
     CDS             124..567
                     /locus_tag="OO568_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6643013.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQAHLGHRSSSMTDKYRDGRGREWKDI"
     gene            803..1622
                     /gene="ybhA"
                     /locus_tag="OO568_003892"
                     /pseudo
     CDS             803..1622
                     /gene="ybhA"
                     /locus_tag="OO568_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
     gene            complement(1623..2681)
                     /gene="modC"
                     /locus_tag="OO568_003893"
     CDS             complement(1623..2681)
                     /gene="modC"
                     /locus_tag="OO568_003893"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6643014.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2684..3373)
                     /gene="modB"
                     /locus_tag="OO568_003894"
     CDS             complement(2684..3373)
                     /gene="modB"
                     /locus_tag="OO568_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6643015.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3373..4146)
                     /gene="modA"
                     /locus_tag="OO568_003895"
     CDS             complement(3373..4146)
                     /gene="modA"
                     /locus_tag="OO568_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6643016.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKANEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4313..4462)
                     /gene="acrZ"
                     /locus_tag="OO568_003896"
     CDS             complement(4313..4462)
                     /gene="acrZ"
                     /locus_tag="OO568_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6643017.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
BASE COUNT         1231 a         1186 c         1081 g         1043 t
ORIGIN      
        1 gaagatccag cttctgcaac caggccattc agaactgaag tgaagcgcag tcgactgtta
       61 attgacgagt atctgttaat caggaaaata gcagacgtcc agaacgaatg gttcggcctt
      121 tgcatggatc ttgcactggt aacaggacag cgcgaaggag atctggcggc tatgagatgg
      181 gaggatataa gagatagcag gctatacgtg gaacaacaaa aaacaggggc aaaaattcgc
      241 atttctctcc ccaccacgat ttccagattg aatttaacac ttgccgatgt actggataac
      301 ttgaaaaaaa taaacggtaa aaacgaaaag ttactcggag ggaagacagc caggacaatc
      361 gcagcacagt tcaggattgc ccgagacaca tccggattga aatgggaagg tgacccaccg
      421 ccatttcatg aaatccgaag tttatccggt cgcctgcact cagcagaaaa aggcagtgat
      481 ttcactcagg cgcaccttgg acatcgttca tccagcatga cggataaata ccgtgacgga
      541 cgagggaggg aatggaaaga catttagaat taatttcgaa tgatttcgaa atactacttg
      601 taatcaattg attataaagg attaattcct aaagagcatt tgcttcatga atgctccttt
      661 gcatttagca actgactgta acagctaaaa ttagtcgctt ttggcggtga atgctgaatt
      721 aaaacgcaga attttgtccg atagtggtat cggtgtacca ttcgcgcaga cgaaaattct
      781 taaccttaat ctggaatacg ccatgaccac acgcgtgatt gctctcgact tagacggcac
      841 cttattgacc ccgaaaaaga ccttgcttcc atcatcgata gaagccctgg cccgcgctcg
      901 cgaagcaggc tatcaattaa tcatcgtcac aggtcgccat cacgtcgcta ttcatccttt
      961 tttatcaggc gctggcgctg gatacacctg ctatttgctg taatggcacc tatttgtatg
     1021 attatcatgc aaaaaccgtg ctggaagcgg acccaatgcc cgttaataaa gccctgcaac
     1081 tcattgagat gctgaatgaa caccacattc acggtctgat gtatgtcgat gatgcaatgg
     1141 tctatgagca cccgaccggg catgtcattc gcacgtctaa ctgggcgcaa accctgccgc
     1201 cggaacagcg tccgactttc acacaagtcg cttctctggc tgaaacgacg caacaagtta
     1261 acgccgtatg gaagttcacc ctgacgcacg atgacctgcc gcaattgcag cattttggta
     1321 agcatgtcga acatgaactg ggactggagt gtgaatggtc ctggcacgat caggttgata
     1381 ttgcacacgg cggcaacagc aaaggtaaac gtttgacgaa atgggttgag gcgcaaggtt
     1441 ggtcgatgga aaacgtcgtg gcattcggcg ataactttaa tgatatcagt atgctggaag
     1501 ccgctggtac aggcgtggcg atgggcaacg ccgatgacgc ggtaaaagcg cgcgccaaca
     1561 ttgtgattgg tgataacacc accgacagca ttgcccagtt catttatagc cacctgattt
     1621 aatcaggcgg ttatcgacac acttttaatt tgcgcgtaca gccacaggcc aggtttgatc
     1681 gccagttcat ccctggccca cgggctgata cgcgcccaca gcgttttacc gccgacttcc
     1741 agctccactt ccacctggcc gttgtcgtca taactattaa caacttttgc ccgcaatacg
     1801 ttacgaatgc tggtttgctg cggcggttgt aaaaccagag aaacatcgga agcctgaatg
     1861 cggatacgta gcgcagcttg cagcggttcg cccagcttat tgacccacaa atgctgatcg
     1921 cccagcgcca gcgcggtcat cgcgtaatgc ggatgatgct caagcaccgt cactttcaga
     1981 atgctacttt gttgctcttt cggcagccac ggattcatca cgctactgcc ccacacttcc
     2041 tccagcgcgc caaaggcttt cacctgaccg ttttccagta ccatcactct gtctgccaga
     2101 tggaggatct catccagcga atggctgaca tacaacatcg gaatgttgat ttcccgtgtc
     2161 agccgttgca gataaggcaa cagttcgcgt ttacgcggaa tatccagtga cgccagcggt
     2221 tcatccagca acagcaattc cggtgctgtc agcaaagccc gaccaatcgc cacgcgctgt
     2281 ttttcgcctc cggacaggct gcctggtaaa cggtcaagca acggttcaat gcctaaaagc
     2341 gccaccagct tatcgaactg atcgaccata cttttcgaca tgccgtagcg cagattgcca
     2401 cgcactttgt aatgcgggaa caaccgcgca tcctgaaaaa catagccaac acgacgcttt
     2461 tccggcgtca ggcagatacc tttttcggca tcatttagta cccgcccatt gaggacaatc
     2521 cgcccttttt gcgggcgcgt cagtccactg atggcgttaa tcagcgaagt ttttccggca
     2581 ccggagacgc caaagatagc agtgatgcca ttggcgggca gcgtttcatt aatggtcagg
     2641 caatggttgc ccaacgtctg ggaaaaattc agctccagca tgattagcgc cccgcccgtt
     2701 cacggctgat tctggccagc cattctgaaa tcaacaggga gatcatcgcc agcgcaatag
     2761 aaataatgca cagtctcgcc gcgccacttt ccccgccggg ggtctggatc agggtataca
     2821 tggcagaagg aatggttcgc gtttcgctag gaatgttcga cacaaaggtg atggttgcac
     2881 caaactcacc gagagaacga gcaaaagcca gtaccgtacc aacaataatt cccggtaagg
     2941 tcagcggtaa cgtgatagta aagaaaacgc gccagcgccc ggcacctagt gttcttgcgg
     3001 cctgttccag tttgacatca accccttcca gcgccagacg aattgcccgc accatcagcg
     3061 gaaacgacat gacggcggca gcgagtaccg cgccgcgcca gctaaaggcg aaggtaatac
     3121 caaaccagtc atacagacgt tcaccgataa atccgcgccg tcccatcgaa actaataata
     3181 agtaaccgac gaccacgggc ggtaacacca gcggtagatg cagtacgctg tcgagcagag
     3241 ctttgcccgg aaacgtgcaa cgcaccagta accaggcaaa aaagatccca aacggcaggc
     3301 taaacagcac agccagggaa gaaactttca ggcttaataa aactgcctgc cattctggat
     3361 cggtcagtat cattacttgg ttgtaaatcc gtaacgttta aagatttccg ctgcctgcgg
     3421 tcccttcaga taatcataaa atgctttcac tgtcgcattg ttatgccctt ccacaacagc
     3481 aaccggatat tccacttttt tatgtgaatc ttccgggaag gtggcaacca cttttacccc
     3541 tttgctggca actgcgtcag aaccgtagac aatgcccaga ggcgcttcgt tacgttcgac
     3601 cagcgccagc gccccacgaa catcttccgc tggggccagt ttcggagaga gcgtatccca
     3661 tgcgcccagt ttttgcagtg cttcttttgc ataaatgcca gcgggaacat gttccggatc
     3721 gccaaccgcc aggcgaccgc cattcagcag cgaagtccag ttagttttgc tgtcgatggt
     3781 gaaatctttc tgctcgttgg ctttcggtgc tacaacgacc aggctattgc cgagcagtgt
     3841 ctgacgcgta gcagtatcga tcgctttttt atcaaccgca taatccatcc atttctgatc
     3901 ggcagaaata aacagatccg caggggcacc cgcttcaatc tgacgggcga gagtagatga
     3961 cgaagcgaaa gaagaaacca catccacgcc tttctctttt ttatactgcg tagcaatgtc
     4021 ctgcattgcg ttagtcagtg atgctgcggc gaacaccgtg attttccctt catctgccag
     4081 tgcattacca gcaactgcga aagagagtgc cgccccggca aacaagttca accatttacg
     4141 agccattggt aaccccttaa tgtaacgtta tgtaggcgac aatataacga ctaactcgga
     4201 attttcccag ctgttattgc taacctttgt gaggtagata agaaaaaata tcggcaggaa
     4261 aagcaggaag ttgagaaaaa gaaaatgccc gactaagcgg gcattcaggg aatcaatgat
     4321 tttgtccggg ctggtctttt ttaccaacac cagaaaagat gttgaatact tcaccaagac
     4381 cgtaaatcag acccaggatg atggccatca cgacaggtac catgattacg gcgaatacca
     4441 gactttttaa taactctaac atggtcaact ccagatatag tcatgagact attctaaccg
     4501 ctaagcacag aaaagggtaa tgctgccaac ttactgattt a
//
LOCUS       DAJTYG010000187         4524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTYG010000187 DAJTYG010000000
VERSION     DAJTYG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.189"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..253
                     /locus_tag="OO568_003897"
                     /pseudo
     CDS             1..253
                     /locus_tag="OO568_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..1492
                     /locus_tag="OO568_003898"
     CDS             746..1492
                     /locus_tag="OO568_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6643018.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1507..1929
                     /locus_tag="OO568_003899"
     CDS             1507..1929
                     /locus_tag="OO568_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6643019.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGKFQEAVL"
     gene            1987..2343
                     /locus_tag="OO568_003900"
     CDS             1987..2343
                     /locus_tag="OO568_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643020.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2436..2654
                     /locus_tag="OO568_003901"
     CDS             2436..2654
                     /locus_tag="OO568_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6643021.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2656..3018
                     /locus_tag="OO568_003902"
                     /pseudo
     CDS             2656..3018
                     /locus_tag="OO568_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3018..3254
                     /locus_tag="OO568_003903"
                     /pseudo
     CDS             3018..3254
                     /locus_tag="OO568_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3447..3683
                     /locus_tag="OO568_003904"
                     /pseudo
     CDS             3447..3683
                     /locus_tag="OO568_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3683..4048
                     /locus_tag="OO568_003905"
     CDS             3683..4048
                     /locus_tag="OO568_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6643022.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT         1212 a          961 c         1286 g         1065 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagatac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggtgaattt
     1441 taactgggag agctggcgtc cgaatgttag ccattcgagg gttgttaagt agtttcagga
     1501 ggatttatgg cgaaaccttt tactcccgaa cagcgggaag aactgaagac gcgaattgtg
     1561 gaactcgtgc atcaggacgg tcgggtcacg attcggcagt tgtcagatga aacaggtatc
     1621 agtcgtgcgt ctgtcggtcg cttatgcatg gaactggtcg caagtggtga tgtatataat
     1681 tctggctacg gggtatttcc ctctgaacag gctcataaag actggcaaaa cgcccgcaaa
     1741 aagctatcga gggtaaaggt gaagaaaccg gttgtggttg atcctgacct tatctggtca
     1801 ttaccagacg gagaaatacg ccgctacgac aggcgcctga atataatctg tcgcgagtgc
     1861 cggaagagcg aagctatgca gcgcgtgctg gcgttttatc aggggaaatt tcaggaggcg
     1921 gtactgtgag taaaattagc tatcaggctt caattaccgc tggtattcgc atcaaaggag
     1981 aggagcatgg aaataaaacc agaagatgag ttaagcaata tcgttttatt tccggtaaaa
     2041 gaggatgacc cacgtaatca ggttaatttt ctttatgaac catcggaaag accatattgc
     2101 catcacgcct ctgtccgggt tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt
     2161 gcggttgtgg agccgtttga ctggatgctc tctgtggcaa aaagagaaac cagactggca
     2221 gatgatgtaa ggctattgcg ccaggaggaa caggaaaggc ggagaaatat agaaaagctg
     2281 atacagattg agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa
     2341 taattaaatt tagcactgtt aaaaatttaa tccttaaccg gagggatttc tgcaccctca
     2401 aatcatcagg agaccgcccg aaagggcggt aatgaatggt tacattattt agaaaaaaat
     2461 atccgcgaaa gagtagaaca acagaattcc tgtttctcat tctgtttatc gtgttgatga
     2521 caccgatatc cccgctaatt tttgtctgga taatcggaaa aataattgag ccagttattg
     2581 aattgtataa cgacgtggta tgggcgtcat tcaacacact gcacaataaa attaatccgt
     2641 ataaggaaag ctgatatggc actgacgaaa aaacaacgtg cagaactgcg catgaagttc
     2701 ggtggtcgct gcgcttattg cggctgcgaa cttggcgaaa agtggcatgc agaccatgta
     2761 aaaccggtca ttcgttttga tggaaatatg cttcaccagg aacgtgacga tatatccaac
     2821 atggtaccag aatgccaccc atgcaatctg cacaagcatt gcagtagcct agaagattat
     2881 cgacgaatta tcagtgatgg tcgtcgtgaa ttccttgcgt ccgggaaagg caaagcgctg
     2941 gttcgtatgg gattggttga aatgaaatct gacccggttg tgttctggtt tgaaaaatat
     3001 caagaagggg ctacggcatg gccactatta ccaaagagcg actgcaatgg ctggctaaca
     3061 tttctggccg cgatgatatt gacgatatag acggcggtga aattcgtgaa ctggcgctta
     3121 tcgctctggc atcactggaa gcagagcctg tagcggaatg cattgttgaa gatgggggca
     3181 tgtgtattga cgggttcggt gagtatgtgg gtcactcgct gcctgatgga atgcatgagc
     3241 tttatgctgc cccgcgaatg cgacaacctg atggatggaa agccgtagct gtagcgtgga
     3301 aggtgacgtt tactcaagtt gaccaggaat ctaatacgtt cactgctata tattttgaca
     3361 aagcggaagt tgagcgttgg gtacgactgc ataaagcatg tgattttcgg gcagatataa
     3421 caccgcttta cgcagcgccg gcagtgccgg ttgcagtaaa cgacgacatg gcttacgcat
     3481 tccatcacgc actgtcagat tcatcgcttg gctctggtga aatcgaagaa attaaaaccg
     3541 gtttgcgtgc tgcctttgcc aacgtcacta tccaaccaga gccgatagtg ccggatgaaa
     3601 tcgggccaaa cgatagtaat acgtttgatt atgttgatgg ctggaacgcc tgccgcgctg
     3661 ccatgcttaa gggagataaa taatgattaa tcgaaccaaa ctggagcaca ttctcgaata
     3721 tgccaggcag cagaaatgta ttgggcaact ttgtaaaatt ccaccaggag atatggttga
     3781 aatcgtggaa atggccatgc gtaaggctgg caactctccg gtaactctgg ctggctggat
     3841 aagctgtagt gatgcagttc ctgctgaata ttgcgatgtg attcttctcg atgatctcgg
     3901 gaatgtattc cccggttcct gggataaggt tttttgcccc actcgtggcg ggaataagat
     3961 ggcttttgtg gacaaagacg gcgttgaagt agagagctca actcactgga tgccgctacc
     4021 ggaaccaccg caggaggtga atcaatgacc tggcctgagg cattcaccac ggtaggaatt
     4081 gcgatggcgg tggcgctggt ggtgtattcg atttgccgtt ggggttaaca aacaaaaacc
     4141 ccggattgat ggtccggggt ttttgaagga aacaaaacag aaacagcaat tgccgttacc
     4201 tgttgttacc atggcaagta aacgtatctc aggcgagcgc attgcgccgt tctgacgcag
     4261 ataaattagc ctggatagaa ggtgctggca ataaaaaata gcgttttctt atcggtatcg
     4321 gtaagattgc tgcgggtgct tgaggctgtc tgcctcgggc atgccactgt aaggcagaca
     4381 gagaaaagcc ccagttaaca ttacgcgtcc tgcaagacgc ttaacattaa tctgaggccc
     4441 aatctatgtc tcacaaatgt aggttagcct cttacgtgcc gaaaggcaag gagaagcagg
     4501 ctatgaagca gcaaaaggcg atgt
//
LOCUS       DAJTYG010000188         4508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTYG010000188 DAJTYG010000000
VERSION     DAJTYG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.190"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            368..2020
                     /locus_tag="OO568_003906"
     CDS             368..2020
                     /locus_tag="OO568_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6643023.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="OO568_003907"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="OO568_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2334..2453)
                     /locus_tag="OO568_003908"
                     /pseudo
     CDS             complement(2334..2453)
                     /locus_tag="OO568_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2527..2961)
                     /locus_tag="OO568_003909"
     CDS             complement(2527..2961)
                     /locus_tag="OO568_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HCR6643024.1"
                     /translation="MDTFKRRNVATSFSFLGYDFKVRTLKNFKGELYRKCMPGASNAA
                     MCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFSYRLWSAMQSR
                     LLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYLLRASNE"
     gene            complement(3138..4447)
                     /gene="ltrA"
                     /locus_tag="OO568_003910"
                     /pseudo
     CDS             complement(3138..4447)
                     /gene="ltrA"
                     /locus_tag="OO568_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1197 a         1090 c         1039 g         1182 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagagcaacg gaagccttgc aaacggggat gcgttcatac
      361 aggtacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc gtcatacggc
     2521 tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct tcggatactg
     2581 tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt atttagactg
     2641 catccactta agcaaacgtg actgcattgc actccacagt cgatagctga agtttctgga
     2701 ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc gcctcgcaaa
     2761 atccagcaaa ctttcagctg ttgagcgatg tatacgccac ttcttgattg tttctgttat
     2821 tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt cgcctttgaa
     2881 attcttcaga gtacgcactt tgaagtcata tccgagaaag gagaaactcg tagcgacgtt
     2941 tctcctttta aacgtgtcaa tgtaggcgat attcgtcttc cctgcgttca ggaccaaccc
     3001 gacttccgag aagcgcaccg ataacctgtt cttcagcctt gttgcatcac tcattcgtga
     3061 acaatgtact acaagtcgag tcacccgagg gaacctcccc ctctggtgct cacggaaccg
     3121 gacgtgaacc tctcagctca tccggctctt ctcattgagg cctgtcccca tagtaacggc
     3181 cagtgtgcaa ataacctcgt ttcactccgc gcaacacggg ccagccagtg gctggcccgt
     3241 cgtcggtggc ccctaagtcg cttgtgttta cggggcagcc ataacacgag tttgcgatcg
     3301 atttgcctca gcgtcagata cagtgctgat ttataaaaag caccgtagta ctttatccag
     3361 cctctgattt tcgcattgaa catatgcgcc agctcgtaca aggacttgtc actgcgattc
     3421 tcctcacctc ctgacggatt gcttttcctg ctctggcact catcgccgga ctgaagttaa
     3481 cgaaggtctt accccagcga ttcatcgaca gacgcggccg gaatgtatag cccagaaagt
     3541 tgaagctcgt ttccgggtag tccccccgtc ggtcagcatc cttacagtaa actattcgtg
     3601 ttttttgcgg atgcagctgc agcccgcatt gcgcaagccg ctgcctcagt ccacttatca
     3661 gagcgtcagc ctgccactgg ctgtgacagt ggcatacaac atcatcggcg tacctttcga
     3721 agggaacgcc gggaaactga cgttgcatcc acatatcaaa ggcatagtgc aggaacagat
     3781 ttgagagcag gggggagatt acgccgccct gcggcgttcc tcgaccccgt gccagcattg
     3841 ctccatccgg catttgcacc ggtgccttca gcctgcgctc aatgtacatc accacccagc
     3901 gtgcctgagt atgatgctgc actgtcttca gtagcaggtc atgatcgata tcatcaaaga
     3961 agcctttaat atcgacttcc accacccagt caaacttcca gcagcgtttc ctcgcctgcg
     4021 caatcgcctg gtgtgaggat ttacccggtc gataaccata cgagtctgca tggaacaggg
     4081 gttccagtac cggctcgagt atctgcttaa cgaccatctg ggcgatccgg tccgacacgg
     4141 ccggaatacc gagcggacgt acaccaccgt cagctttggg aatgtccaca cgccttaccg
     4201 cctgcggcat ataactgccg gaagccattc tgttccacag tttgtagaga ttgtctgtaa
     4261 cattctcgtc aaacccggcc agtgtctgcc cgtcaatgcc tgccgcccca cggttggctt
     4321 ttactcgttt ataagcttcc catacctgtc gtttacttac aggaaatggc tttgccgatt
     4381 ttacagcagt cctcccatcg gcattgtcgc ccgtggttgc cgccgcacca tcagcacaat
     4441 ggggcaagcc ctttgcttcg gacccattac ggtccttcac cgctactacg gtttactccg
     4501 tccctgta
//
LOCUS       DAJTYG010000189         4471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTYG010000189 DAJTYG010000000
VERSION     DAJTYG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.191"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(77..754)
                     /gene="ycjM"
                     /locus_tag="OO568_003911"
                     /pseudo
     CDS             complement(77..754)
                     /gene="ycjM"
                     /locus_tag="OO568_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810499.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(966..1280)
                     /gene="pspE"
                     /locus_tag="OO568_003912"
     CDS             complement(966..1280)
                     /gene="pspE"
                     /locus_tag="OO568_003912"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6643025.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(1355..1576)
                     /gene="pspD"
                     /locus_tag="OO568_003913"
     CDS             complement(1355..1576)
                     /gene="pspD"
                     /locus_tag="OO568_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6643026.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1585..1944)
                     /gene="pspC"
                     /locus_tag="OO568_003914"
     CDS             complement(1585..1944)
                     /gene="pspC"
                     /locus_tag="OO568_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6643027.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1944..2168)
                     /gene="pspB"
                     /locus_tag="OO568_003915"
     CDS             complement(1944..2168)
                     /gene="pspB"
                     /locus_tag="OO568_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6643028.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2222..2890)
                     /gene="pspA"
                     /locus_tag="OO568_003916"
     CDS             complement(2222..2890)
                     /gene="pspA"
                     /locus_tag="OO568_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6643029.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3057..4034
                     /gene="pspF"
                     /locus_tag="OO568_003917"
     CDS             3057..4034
                     /gene="pspF"
                     /locus_tag="OO568_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6643030.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLQDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4154..>4471)
                     /locus_tag="OO568_003918"
     CDS             complement(4154..>4471)
                     /locus_tag="OO568_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6643031.1"
                     /translation="ESLCERANQLGQRLKNTLIDAKESVPAIAAVRGLGSMIAAEFND
                     PQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDAQFDAAMKILQDA
                     LSD"
BASE COUNT         1113 a         1111 c         1060 g         1187 t
ORIGIN      
        1 agcaaaagtg gtgttgaaga aacatccgtt ttgtggtgtt tttttaatct ttttggggtt
       61 ttaattccta tcgatggaca tactcagcac ctttctcaag gtaacagagt aaaacatcca
      121 ccatcgccag caacacttca gggctacggt aattcaggtc aatttgatcg tcactaaagg
      181 tggtccataa atggcgcgtg gaattatcat ccatttgaaa cggcgttaat agcggtaacg
      241 cacgcggacg ggtgacggca ctgagatcgg ttttcggatc aaccgcaata aagaaatctt
      301 caaaacccgg aagctgttgt aaatagtttt taaaccattc actttttgca gacatatggt
      361 tgcagacaaa atcaaacatt aaatgactgc attcaccgag ttgctgaata tcctgccact
      421 cccccgcttc actggcgacc tgatgataat caattaccga aaatccatca tcagatgacc
      481 atgggtaaaa aggtaataaa tgaacatgtg aaaaaatgct ttgcagccat tgatggtaga
      541 actgattaaa tatgggtagt ggttttaaat cattactgtg aaattgatcg gcataggtaa
      601 ttaacacgac atcactttca tcccagtgtt ttttacgtcg ctgtgtaatt aatcgtttcg
      661 cactctcaag acgcgttaca agtttctgta aatgagttgc ggtaaatgtt ccaccgtaga
      721 tttcgtccag gtaatccgta attttctgtt tcatgttgcc tttccgtggt agcggtccca
      781 tgaggcgaga gactactccg cagaaaataa actgtcaatt gagcacaagg gttttagtca
      841 cgcaggaaat cattttttac cagggaaaaa gcgtatttgt gagctgacgc aaataatcta
      901 tagggatgta aataaggtgt gacagaaaaa aaacggcgca taagcgccgc tcatggtgaa
      961 ttcttttaac ctttgacctt cggcattgcg atgtctttca ggccaccggc gttctcaacg
     1021 tgggtatatc ccatctcgct aaggatctct tttgcctgcc cggactggcg tccggcattg
     1081 caatacactt tcacggtgtc gtttttatcc ggaacggcgg tggcaatgcg ctctttcact
     1141 tccttcaggg gaatattgat ggccccctga acgtgctctt gctgatactg ctctggaaca
     1201 cgaacatcga tccagtgttc agcggcgaaa acgggcagtg aaaacaccag tgccagagca
     1261 agtaagcctt ttttaaacat gtaagcgtcc ttctatggat gaaaattgcc atccattata
     1321 cgcctgagat ttaacaattt ttgcgaaact cccctcacct tttataacgc tgtgccagtt
     1381 tattagcagc ccgacttaac agcggttcca gcgccacagc cagcaacatt ttcagcggtc
     1441 ggcgagcaac tgattttatc gcccagcccg ccacacccgc cgggccatag cgcagtgcgg
     1501 taagaagtac cagcttgcct gctaatttga aaccaagctt caccttttgc ccggcctgtt
     1561 gccagcgagt attcataact ttcctcacag ttgacggaaa cggctacgta acgtgaaagt
     1621 atcggaagtg acataacgtt ccatctcgcg taaacgcgtt tcacttgccg ccagttcacg
     1681 gtcgacttca tccagcaatt cgctgctgga aggtagctgc tcaccaaagg ccatgttgtc
     1741 cggcattgga tcaagcgcaa atgacaaaat gatgtaagca accagggtaa acagcgccag
     1801 accgaagaaa atcgacagca ccaccaggat acgcaccagt tttaccggta catcaaaata
     1861 gttggcaatc ccggcgcaga cgccgcggac catgccctgc tgtggaatac gccataattt
     1921 tttattgaga ttaatgcccg ccattagcga tccctccagt tcggatgttc tgcatcaaga
     1981 atagattcca gcgcctgaat acgttcgcgc atccgttttg cttcatcaac cagttgcgct
     2041 aatcgctgct gctcactttg cgacaattca ctgcgaccag aacgattgct gtaatgcagc
     2101 cataaccaga tcggtaaaac aaacagcaca aaaatggtta acggaatagc cagaaatagc
     2161 gcgctcataa gtactcctta gacgatgagc ggcggcgcgt tcggacgccg cctggatgtt
     2221 attattgatt gtcttgcttc attttggctt ttaattgtgc cagttgttcg ctgattgcat
     2281 catcggcttt cagttcggca aactgatcgt ccagcgattt ttgtttaccg aagctgtggc
     2341 tttctgcttc cgcttccatc tggtcaatac gacgttcgaa agattcgaaa cgagccattg
     2401 cttcatccag tttgccactg tccagctgac gacgcacatc gcgcgacgag tttgccgcct
     2461 gatgacgtaa catcaatgcc tgctggcgag cgcgtgtttc gctcaatttg ttttccagct
     2521 cgccaatctc tttcttcatg cgtgccagcg tatcgtccac cagcgtcact tcatgttcca
     2581 gggacttaat cagatcggtc agtttctgtt tttcaattaa cgctgcacgt gccagatcct
     2641 ctctctcttt cagcagcgcc agttcggctt tttcctgcca ttcaacttca cgcgccgacg
     2701 cttgttcaat acggcgagtc agctgtttct tttctgccaa cgcacgcgcc gaagtagaac
     2761 gtacttcaac cagtgtatct tccatctcct ggatcatcag acgcaccagt ttctgtggat
     2821 cttccgcttt ctctaacaga gcgttgatgt tggcattcac gatgtcggca aagcgagaaa
     2881 aaatacccat aatgttgtcc tcttgatttc tgcgttcagg attgtcctgc tgaactgtta
     2941 atacaatttg cgtgccaatt ttttatcttt ttgatttata aagatctgat tgaagaatca
     3001 acagcaacat gccaggatga attagcgaat tacactaaca agtggcgaat ttcatcatgg
     3061 ccgaatacaa agataattta cttggtgagg cgaacagctt tctcgaagtg ctggaacagg
     3121 tttcgcatct cgcaccgctg gacaaaccgg tgctcatcat cggcgaacgc ggcaccggta
     3181 aagagttgat tgccagccgc ctgcattatc tctcctcccg ttggcaaggg ccgtttattt
     3241 cccttaactg cgcggcgtta aatgaaaatc tgcaggattc cgaactgttt ggtcacgaag
     3301 cgggggcgtt taccggtgcg caaaaacgtc atcctgggag atttgaacgt gccgacggcg
     3361 gtacgctatt tcttgatgaa ctcgctacgg caccgatgat ggtgcaggaa aaattattgc
     3421 gtgtgattga gtacggtgaa ctggagcgcg ttggcggtag ccagccgttg caggtgaatg
     3481 tgcggttggt atgcgcgacg aatgccgatc tcccggcgat ggtcaatgaa ggtacttttc
     3541 gcgctgacct gctcgaccgg ctggctttcg atgtagtgca actgccgccc ctgcgcgagc
     3601 gcgaaagcga cattatgctg atggcagaac actttgccat tcagatgtgt cgggaaatca
     3661 agctgcctct gttcccgggg tttacggagc gcgccagaga aacattgctg aattatcgtt
     3721 ggccgggaaa tattcgtgaa ttgaaaaacg tggtggaacg ttcagtgtat cgccacggca
     3781 ccagcgatta tccgcttgat gacatcatta ttgatccctt taaacggcgt ccacctgaag
     3841 aagccatcgc cgtttcagaa aatacctcgc ttccgacgct gccgctggat ttacgcgagt
     3901 ttcagatgca gcaggaaaaa gagttgctgc aactcagttt gcaacagggg aaatataacc
     3961 agaagcgcgc ggctgaatta ctggggttaa cttatcatca gttccgcgcg ttgttgaaaa
     4021 agcaccagat ttagcgaagt tgagcaatac aacttgccgg atgcggcgtg aatgctttat
     4081 tcggcataca taatcaatac gtcgtttgca ggcctgataa gcgcagcgca tcagacatta
     4141 ttgcgttggg ctattaatcg ctcagcgcat cctgcaaaat tttcattgcc gcatcgaatt
     4201 gcgcatccgg gatggtcagc ggatacagga agcgaatcac gttgccgtat gcgccacagg
     4261 tcagcaggag cagcccctgc gccagcgcgc gttgctggat tttctgtgca atcgccgctg
     4321 acggctcgcc cgtttgcgga tcgttaaact ctgccgcaat catcgacccc aggccgcgta
     4381 ccgcagcaat ggccggaacg ctttctttgg catcaatcaa cgtgtttttg agacgctggc
     4441 ccagttgatt cgcgcgttcg cagagtgatt c
//
LOCUS       DAJTYG010000190         4439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTYG010000190 DAJTYG010000000
VERSION     DAJTYG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.192"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..933
                     /locus_tag="OO568_003919"
     CDS             <1..933
                     /locus_tag="OO568_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6643032.1"
                     /translation="HNISPRFMTEWDIFADQTSMITAGYNRYYGGNILDMGLRDIRNS
                     WTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARANYVYREAHDQISKSSRT
                     DSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFSYIKSKGNLSLNN
                     GYEENNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPSGLVWANTLAWQE
                     ARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQFLKQQNLTISADI
                     LNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            990..3773
                     /locus_tag="OO568_003920"
     CDS             990..3773
                     /locus_tag="OO568_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6643033.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALQQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGAKTWPGNKVIETFESMG
                     LRFGLDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     SEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKFPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTIENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            4135..>4439
                     /locus_tag="OO568_003921"
     CDS             4135..>4439
                     /locus_tag="OO568_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6643034.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT         1341 a          993 c          992 g         1113 t
ORIGIN      
        1 cacaatatct ccccgcgctt tatgacggaa tgggatattt ttgctgatca aacctcaatg
       61 attaccgccg gttataaccg ttactatggc gggaatattc ttgatatggg attacgtgat
      121 atccgcaata gctggacgga atcggtatca ggtaataaaa ccctgacgcg ttatcagaat
      181 ttgaaaacgc cttataacga tgaactggca atgggattgc agcaaaaaat cgataagaac
      241 gttattgcac gcgcaaacta tgtttaccgt gaagcgcatg atcaaatcag caaaagcagt
      301 cgtaccgaca gcgcgactaa aaccaccatt actgaatata acaacgatgg caaaaccaaa
      361 acgcattcgt ttaacctcag ttttgaactg gccgaacccc tgcatatccg ccaggtagat
      421 attaacccgc aaattgtctt tagctatatc aagagcaagg gcaacttgtc gttaaacaat
      481 ggttatgagg agaacaatac cggtgataac caggtggttt ataacggtaa tctggtctct
      541 tacgatagcg ttccagtggc agattttaat aacccattaa agatctcctt aaacatggat
      601 ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc tggcctggca agaagcgcgt
      661 aaagctcgca ttatccttgg taagacaaat gcgcaataca tcagcgaata ttcagattac
      721 aagcagtatg ttgacgaaaa actggatagc agcctgacct gggacacccg cttgtcctgg
      781 acgccacaat ttctgaaaca acaaaacctg acgatcagtg ccgatattct caatgtactg
      841 gatagcaaaa ccgctgttga tacaacgaac accggtgtgg cgacctacgc cagtggccgt
      901 actttctggc ttgatgtcag catgaaattt taatagttcg gattttactt aaccgggtaa
      961 attcgcccgg ttttcgcatg gagataatta tgagaaacct ctgtttctta ctgacgttag
     1021 tggcaactct gttgctcccc gggcggctga ttgccgccgc cttacagcag gatgaaaagt
     1081 taattaccgg gcaactggac aatggcttgc gatatatgat ttatccgcat gctcatccaa
     1141 aggatcaggt aaatttatgg ctgcaaattc ataccggttc attgcaggaa gaagacaatg
     1201 agcgcggcgt ggctcatttt gtagaacata tgatgtttaa cggcgcaaaa acatggccgg
     1261 gtaataaagt catcgaaaca tttgagtcaa tgggcctgcg ttttggtctc gatgttaatg
     1321 cctataccag ctatgacgaa acggtgtatc aggtgagttt gccgactacg cagaaacaaa
     1381 atctgcaaca agtgatggca atcttcagtg aatggagtaa tgccgcaacc tttgaaaaac
     1441 tcgaagtaga cgctgaacgt ggcgtaattt ctgaggaatg gcgtgcccat caggatgcga
     1501 aatggcgcac ctctcaggcg cgccgccctt tcctgctggc taatacccgt aatttagacc
     1561 gtgaacctat cggcctgatg gatactgtcg ccacggtcac accggcacaa ttgcgccaat
     1621 tttatcaacg ctggtatcaa ccaaataata tgacctttat cgtggtcggc gatatcgaca
     1681 gtaaagaagc gctggcgctg ataaaggata atttaagtaa gtttccggct aacaaagcag
     1741 ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct gcgctttaat atcattaatg
     1801 ataaagaaaa ccgggtgaac ggcatcgcac tctattatcg cctgccaatg gtacaagtga
     1861 acgatgagca aagctttgtc gaacaagctg aatggagcat gttagttcag ctgttcaatc
     1921 aacgtctgca ggaacgcata cagtcgggcg agttgaagac tatttctggc ggcactgcgc
     1981 gcagcgttaa aattgcaccc gattatcagt cgctgttttt ccgtgtaaat gcacgagacg
     2041 ataatatgca ggatgctgcg aatgcattaa tggcagagtt ggcaaccatt gatcagcatg
     2101 gtttttctgc tgaagaactc gatgatgtca aatctacccg cctcacctgg ctgaaaaatg
     2161 cggttgatca gcaagctgaa cgtgatttac gtatgctgac cagtcgcctg gcatccagct
     2221 cattaaataa tacgccgttc ttgtcgccgg aagagacata tcaactttcg aaacgtctgt
     2281 ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca gcagttaaga aagaaccagg
     2341 acgcattttg ggagcaaatg gtaaacaatg agcttgccgc caaaaaagca ttgtctcctg
     2401 cggctatcct ggcgctggaa aaagagtacg ccaacaaaaa gctggcggct tacatcttcc
     2461 caggcagaaa tttatcgtta acagtagacg ctgacccaca ggcggaaatt agcagcaaag
     2521 aaacgctggc tgagaatctg acatcattaa cactttccaa tggtgccaag gttattctgg
     2581 caaaatccgc gggtgaagag caaaagctac aaattattgc cgtatcgaat aaaggcgatt
     2641 taagtttccc tgcgcagcaa aaatcactta tcgcgctggc aaataaagca gttagcggaa
     2701 gcggcgttgg cgaactctcc tcttccagcc tgaaacgctg gagtgcggaa aactcggtaa
     2761 ccatgagcag taaagtcagt ggcatgaata cgttgctctc cgttagcgcg cggactaata
     2821 accctgaacc tggtttccag ttgattaacc agcgaatcac ccacagcacg attaacgaca
     2881 atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt gaaaacgctc gaccagcgtc
     2941 cagcggagaa attcgcccag ctgatgtatg agacgcgcta tgctgatgac cgcacgaaat
     3001 tactgcaaga aaatcagatt gcacagttta ctgccgcaga tgcgctggct gccgatcgcc
     3061 agttgttttc atctccagcg gatatcacgt ttgtcattgt cggtaatgtc gcagaagaca
     3121 aactcgtggc gttaattacg cgttacttag gctcaatcaa acactctgat tcgccattag
     3181 ccgcaggtaa accattaact cgcgcgacgg acaacgcatc ggttactgta aaagaacaaa
     3241 atgaacctgt ggcacaggtt tcacagtgga agcgttatga ttcccggaca cctgttaatc
     3301 tggcgacgcg tatggcgctc gatgctttta acgtcgcact ggcaaaagat ctacgcgtta
     3361 atattcgtga acaggcatct ggggcataca gcgtttcttc tcgcctctcg gttgatcctc
     3421 aggctaaaga tatcagtcat ttgctggctt ttacttgtca accagaacga catgatgaac
     3481 agttaacgtt agcgaatgaa gtgatggtta agcgtttgac taaggggatc agtgagcaag
     3541 aactgaacga ataccagcaa aacgttcagc gcagcctcga tatccaacag cgtagcgttc
     3601 aacaattagc gaacactatt ataaatagtc ttattcaata tgacgatcct gcagcatgga
     3661 ctgagcagga gcaattgttg aaacaaatga cgatagagaa tgttaacact gccgttaaac
     3721 aatatctttc tcatccggta aatacttata ccggagtatt attgccaaaa taataacagc
     3781 cccgtcaaca catcgttggc ggggatttta gcaatattcg ctatttttat gtaataattt
     3841 tataaatgcg ttcaaaataa taaccaagta ctaatagtga tattttaatg tctgattttt
     3901 acgtgataat tcaggaggca cagaaagcgc ataaaaataa caacataaaa caccttgcca
     3961 ccacccaaga attctctatt gcattgtttt taatgaaaaa aatatcattc caacaatatc
     4021 tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca cttgcttact
     4081 ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt taaaatggat
     4141 aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg tgcgaagtct
     4201 atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga cgatgtcgca
     4261 tttcagatta tcaatgatga attatatctt gatggcaacg ctcgtcagaa cctggccact
     4321 ttctgccaga cctgggacga cgaaaacgtc cacaagttga tggatttatc cattaacaaa
     4381 aactggatcg acaaagaaga atatccgcaa tccgcagcca tcgacctgcg ttgcgtaaa
//
LOCUS       DAJTYG010000191         4429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTYG010000191 DAJTYG010000000
VERSION     DAJTYG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.193"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..213)
                     /locus_tag="OO568_003922"
     CDS             complement(<1..213)
                     /locus_tag="OO568_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR6643035.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="OO568_003923"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="OO568_003923"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6643036.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLGGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="OO568_003924"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="OO568_003924"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6643037.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="OO568_003925"
     CDS             2894..3634
                     /locus_tag="OO568_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6643038.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="OO568_003926"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="OO568_003926"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6643039.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4192..>4429)
                     /locus_tag="OO568_003927"
     CDS             complement(4192..>4429)
                     /locus_tag="OO568_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643040.1"
                     /translation="FDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFLGSIPWE
                     RKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1095 a         1105 c         1232 g          997 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctgggagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtctt acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctgg cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tctggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaag
//
LOCUS       DAJTYG010000192         4340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTYG010000192 DAJTYG010000000
VERSION     DAJTYG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.194"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..324
                     /locus_tag="OO568_003928"
     CDS             <1..324
                     /locus_tag="OO568_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR6643041.1"
                     /translation="DDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIP
                     CPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIP
                     SQADF"
     gene            383..586
                     /locus_tag="OO568_003929"
     CDS             383..586
                     /locus_tag="OO568_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCR6643042.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRELFTCNKTLPPHEVEPNFGAI"
     gene            586..1611
                     /locus_tag="OO568_003930"
     CDS             586..1611
                     /locus_tag="OO568_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6643043.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(1664..1753)
                     /locus_tag="OO568_003931"
     tRNA            complement(1664..1753)
                     /locus_tag="OO568_003931"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1717..1719),aa:Ser,seq:cga)
     gene            1847..2644
                     /gene="mtfA"
                     /locus_tag="OO568_003932"
     CDS             1847..2644
                     /gene="mtfA"
                     /locus_tag="OO568_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6643044.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2745..2820
                     /locus_tag="OO568_003933"
     tRNA            2745..2820
                     /locus_tag="OO568_003933"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2778..2780,aa:Asn,seq:gtt)
     gene            2982..4244
                     /locus_tag="OO568_003934"
     CDS             2982..4244
                     /locus_tag="OO568_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6643045.1"
                     /translation="MFLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHLELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGSELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT         1214 a          997 c         1036 g         1093 t
ORIGIN      
        1 gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt
       61 caggtctggg gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg
      121 caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag
      181 ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat
      241 aatgcacttg atgacgctcg ttaccaggca aaatacgttt cagctatctg gcaaaaactg
      301 atcccgagtc aggctgattt ttaatgttca accctaattg ccgctaaccg tatatagtta
      361 gcggcggtta tgagatatag ctatgagcag cttattttta accgaagatg aattgctaat
      421 attaacgggc tgcaaatatg caagccacca gcgaaaatgg ttaatggaaa acgggcttcc
      481 gttctatacc aatcgtagtg gcaaaccgat tgtcagccgg gagctattta cctgcaataa
      541 aactttacca ccacacgagg tagagccgaa ttttggtgcg atctgatggg aagacgaagg
      601 aaaaatcctg aacacgaaaa attacctcca aatgtatacc caaataaata tagttatgta
      661 tggaaaccaa catccagaga atctgtaaca ctaaccgcca tcaaggatgg tttagctgct
      721 ttatggaaaa agtatgagga aactgtaaat aatcgcgatc gtgcaatgac attcggtcgc
      781 ttgtgggaaa aattcctcgc cagcgcctat tacagtgacc tcagtccaag aacacaaaaa
      841 gattatctgc aacatcaaaa aaagttgctt gccgtattcg gtaaggtgcc ggcagattcc
      901 ataaaaccag aacacatccg tcgatacatg gacaagagag gggaacagag taaaacgcaa
      961 gccaaccatg aaaaaagcag tatgtcccgc gtttacagtt gggggtatga gcgagggtac
     1021 gtgaaggcta acccatgtgc aggtgtaagt aaattcaagg ccaaaaaccg cgaacgatat
     1081 gtaaccgaca aagaatacca ggcagtatta agcgttgcac ctcttcctgt ttttatcgca
     1141 atggaaattg cctatctgtg tgcagcgagg gtttccgatg tgttatcgct gaaatgggag
     1201 cagattggaa acgacgggat ctttatccag caagggaaaa caggaaaaaa acagataaaa
     1261 gcatggagtc cacgattaca ggcggcgatc gaaaaagcaa aacagttacc aacatccgcc
     1321 tatgtaatca gcaatcaata cggcaaccga tatatgtaca aaggctttaa cgaaatgtgg
     1381 gtagaagcaa gaaatcgcgc aggcaaaatt tcaggtattt taaccgactt cacctttcat
     1441 gatctgaagg cgaaaggaat ttcagactat gaaggaagca gtcgggataa gcaacttttc
     1501 tctggtcaca aaaccgaggg gcaagtgcta atctatgaca ggaaggttaa agtttcaccg
     1561 acacttgatg tcccgttacc tgaaaatatt ccaagaaaat attccaagta attccaagtg
     1621 tgatttttgt cactgactta atgatgtata agtgattgaa ttttggcgga gagaggggga
     1681 tttgaacccc cggtggagtt gcccccactc cggttttcga gaccggtccg ttcagccgct
     1741 ccggcatctc tccgctcaga tggttgccat gatgccagga aatttggcat tttaacagtc
     1801 cctgtccgtg caattttgtt caagtgacga gtttgcgagc aaaacgatga ttaagtggcc
     1861 ctggaaagta caagaatcag cacatcaaac tgcccttccc tggcaggaag cactatcgat
     1921 ccccctttta acgtgtctga cagaacagga acaaagcaaa ttggtcgctc ttgccgaacg
     1981 ttttttacag caaaaacggc ttgttccttt acagggcttt gagctgaatt cattaagaag
     2041 ctgccggata gcacttctat tttgcctgcc cgttctggag ttaggactgg aatggctgga
     2101 tggttttcat gaagtcttaa tttatcctgc gccatttgtg gtcgatgatg aatgggaaga
     2161 cgatatcggt ctggtgcata accaacgtat tgttcagtca ggtcagagct ggcagcaagg
     2221 gcctatcgtt ttgaactggt tggatataca agattctttt gatgcttctg gttttaacct
     2281 gattattcat gaagtcgctc ataagctgga cacccgtaac ggcgatcgcg ccagcggagt
     2341 tccctttatt tcgttgcgtg aggttgctgg ctgggaacac gatcttcatg ctgcaatgaa
     2401 caacattcag gaagaaatcg aattagttgg tgagaatgcg gcgagcattg atgcttatgc
     2461 tgccagtgat cctgctgaat gttttgccgt actttctgaa tatttcttta gcgccccaga
     2521 actttttgct cctcgtttcc cttcattgtg gcaacgtttc tgtcaatttt atcaacaaga
     2581 tcctttgcag agactgcatc acgctaatga tacagactcg ttttcggcga cgaatgttca
     2641 ttaattaaca actttgcaga ttaattaacc aattgaaatg gcttatgaaa tttagtgttg
     2701 acagacaagg taccgctaag taatatgcgc cccgttctca cgattcctct gtagttcagt
     2761 cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca
     2821 attttctgtt ttcatgcatc cttgcgaatc tttatctgat gttgattcaa caggttagtg
     2881 tgaaaactct tctcggtgca ttttgatttt accctacgca tcgggaaaaa ttggtggtca
     2941 aatctgggat caggttagtt cgataatgga gtgaccccca tatattcctt accgacgcaa
     3001 aaatccgcac cctcaagcct tctgataaac cctttaaagt ctccgattct cacggtctgt
     3061 atctgctggt caagccgggt ggctcccgcc actggtatct caaataccgt attagcggta
     3121 aagaatcccg cattgcgctg ggtgcctatc cagccatctc cctgtctgat gcgcgacagc
     3181 aacgtgaagg tatccgtaaa atgctggcgc tgaatatcaa cccggtacag cagcgggctg
     3241 ctgaacgtgg ctcacgaaca ccggagaaag tttttaaaaa cgtggcgctg gcgtggcata
     3301 aaagtaacag gaaatggtcg cagaacaccg ccgaccgtct gcttgccagc ctgaacaatc
     3361 acatctttcc ggtcatcggg aacctacctg tatcagaact taaaccccgt catttcattg
     3421 acctgctgaa agggatcgag gaaaaaggtc tgctggaggt tgcgtcccgc acacggcagc
     3481 acctgagtaa cataatgcgc catgcggtcc atctggagtt aatcgatacg aaccctgcag
     3541 caaaccttgg cggcgtgacc acacctcctg tcagacggca ctatcctgcc ctgccgctgg
     3601 agcggctgcc tgaactgctt gaacgtattg gggcatatca tcagggcagt gaactgaccc
     3661 ggcatgccgt tctgctgatg ctgcatgtgt tcattcgctc cagtgaactg cgtttcgccc
     3721 gctggtcaga gattgatttc acaaaccgag tctggacgat acccgcgacg cgagaaccca
     3781 ttattggcgt gcgttattcc ggccgcgggg caaaaatgcg aatgccgcat atcgtccccc
     3841 tctcagaaca gtccatcgcc attctgaaac agattaagga tatcaccggt aataatgaac
     3901 tgatcttccc cggcgaccat aacccgtata agccaatgtg tgaaaacacg gtcaataagg
     3961 cactgcgggt gatgggttac gacacgaaaa aggatatctg cggtcacggc ttccgggcaa
     4021 tggcatgcag tgcgctgatg gaatcgggtt tatgggcaaa ggacgcagta gaacgccaga
     4081 tgagtcatca ggagcgcaat accgtgcgca tggcttatat tcataaggca gagcacctgg
     4141 aagcccgcaa agcgatgatg cagtggtggt cggattatct ggaagcatgc cgagaatctt
     4201 atgcaccgcc ttatacaatt ggtaaaaata agtttatccc atagtatact aacgaatccc
     4261 acactctggc aaattaccac cacctctaca ggaggtggtt ctatatagac ttttatcaac
     4321 tttatgacga aaaacaccta
//
LOCUS       DAJTYG010000193         4332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTYG010000193 DAJTYG010000000
VERSION     DAJTYG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.195"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..358)
                     /locus_tag="OO568_003935"
     CDS             complement(<1..358)
                     /locus_tag="OO568_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCR6643046.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREH"
     gene            complement(473..548)
                     /locus_tag="OO568_003936"
     tRNA            complement(473..548)
                     /locus_tag="OO568_003936"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(513..515),aa:Thr,seq:ggt)
     gene            complement(555..629)
                     /locus_tag="OO568_003937"
     tRNA            complement(555..629)
                     /locus_tag="OO568_003937"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(594..596),aa:Gly,seq:tcc)
     gene            complement(746..830)
                     /locus_tag="OO568_003938"
     tRNA            complement(746..830)
                     /locus_tag="OO568_003938"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(794..796),aa:Tyr,seq:gta)
     gene            complement(839..914)
                     /locus_tag="OO568_003939"
     tRNA            complement(839..914)
                     /locus_tag="OO568_003939"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(879..881),aa:Thr,seq:tgt)
     gene            1276..2226
                     /gene="coaA"
                     /locus_tag="OO568_003940"
                     /gene_synonym="panK"
     CDS             1276..2226
                     /gene="coaA"
                     /locus_tag="OO568_003940"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6643047.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2255..3220)
                     /gene="birA"
                     /locus_tag="OO568_003941"
     CDS             complement(2255..3220)
                     /gene="birA"
                     /locus_tag="OO568_003941"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6643048.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3217..4245)
                     /gene="murB"
                     /locus_tag="OO568_003942"
     CDS             complement(3217..4245)
                     /gene="murB"
                     /locus_tag="OO568_003942"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6643049.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1106 a         1080 c          979 g         1167 t
ORIGIN      
        1 tgtgctcacg agtctgcggc atcgggccgt cagtcgcagc aactaccagg atcgcgccgt
       61 ccatctgagc agcaccggtg atcatgtttt taacatagtc ggcgtgcccc gggcagtcta
      121 cgtgtgcgta gtgacgggtc ggggtgtcgt attcaacgtg agaagtgttg atggtgatac
      181 cacgagcttt ttcttccggc gcgttatcga tctggtcgaa tgcgcgagca gcaccgccgt
      241 aggttttagc cagtacggta gtgattgcag cggtcagcgt tgttttacca tggtcaacgt
      301 ggccgatagt accgacgtta acgtgcggtt ttgtacgttc aaacttttct ttagacatcg
      361 attgtccctc taagacacgg ataaatcggt gatatcacca catcaaccag gcaacatgcc
      421 cgacttgttg aatgcaataa acagaaggaa aaaacaggga ggagaaaaga agtggtgctg
      481 ataggcagat tcgaactgcc gacctcaccc ttaccaaggg tacgctctac caactgagct
      541 atatcagcac atcttggagc gggcagcggg aatcgaaccc gcatcatcag cttggaaggc
      601 tgaggtaata gccattatac gatgcccgca tcctagaact cggctacctg attttcattc
      661 tgcactgaat atcgagagaa gctctcttta ttcgagccgg taagtgaact tatcgtctcg
      721 ggctacgcca tcgcgtggcc gaaattggtg gtgggggaag gattcgaacc ttcgaagtct
      781 gtgacggcag atttacagtc tgctcccttt ggccgctcgg gaaccccacc ggacttgatg
      841 gtgccgacta ccggaatcga actggtgacc tactgattac aagtcagttg ctctacctac
      901 tgagctaagt cggcatcaag tagcgcgcat tctatggaga catgcgagtt catgcaacta
      961 aaaaattgca taatttgttt tattggtcac attttatgct acacgatgaa gaaacagccg
     1021 aacggagggc agattagcac actttttcaa catcattgtg ctcaacaatg cgctcctgct
     1081 aaaccataat tctttttatc agatggaata tctgtcacat tgcttttcaa cgatagcttc
     1141 ctggcagaga ttttttctta ttattcctcc ccatctggtg ttaccctcct gcccattaac
     1201 ccattcaaca gaactgtgac gcgccatggc aaatatcgct ttgccgatag agctatgacc
     1261 gccagaaaca tgcttatgag tataaaagag caaacgttaa tgacgcctta cctacagttt
     1321 gaccgcaacc agtgggcagc tctgcgtgat tccgtaccta tgacgttatc ggaagatgag
     1381 atcgcccgtc tcaaaggtat taatgaagat ctctcgttag aagaagttgc cgagatctat
     1441 ttacctttgt cacgtttgct gaacttctat ataagctcga atctgcgccg tcaggcagtt
     1501 ctggaacagt ttcttggtac caacgggcaa cgcattcctt acattatcag tattgctggc
     1561 agtgtcgcgg tggggaaaag tacaaccgcc cgtgtattgc aggcgctatt aagccgttgg
     1621 ccggaacatc gtcgtgttga actgatcact acagatggct tccttcaccc taatcaggtt
     1681 ctgaaagaac gtggtctgat gaagaagaaa ggcttcccgg aatcgtatga tatgcatcgc
     1741 ctggtgaagt ttgtttccga tctcaaatcc ggcgtgccaa acgttacagc ccctgtttac
     1801 tcgcatctta tttatgatgt gatcccggat ggagataaaa ctgttgttca gcctgatatt
     1861 ttaattcttg aagggttaaa tgtcttacag agcgggatgg attatccaca cgatccacat
     1921 catgtatttg tttccgattt tgtcgatttt tcgatatatg ttgatgcacc ggaagactta
     1981 cttcagacgt ggtatatcaa ccgttttctg aaattccgcg aaggggcttt tacagacccg
     2041 gattcctatt ttcataacta cgcgaaatta actaaagaag aagcgattaa gactgccatg
     2101 acattgtgga aagagatcaa ctggctgaac ttaaagcaaa atattctacc tactcgtgag
     2161 cgcgccagtt taatcctgac gaaaagtgct aatcatgtgg tagaagaggt cagactacgc
     2221 aaataatttg caggggagcg aatactcccc tttcttattt ttctgcacta cgcagggata
     2281 tttcaccgcc catccagggt tttattattc catcctgctc aagtaataaa gccccctgtt
     2341 tgtctattcc gcgtgaaatg ccaaatattt ctttatcacc aatgataagt ttcactgggc
     2401 gattaataaa attatccagc ttttcccagc gcgacagata aggtgccaat ccttcttgtt
     2461 cgaagagttc caacgcagca cgtaattcac gtattagcat ggccgccaac gtattacgat
     2521 caagattgat ccccgcttcc tgcagcgtga tccacccctg attaacgaca ctctcttcaa
     2581 cacggcgcat tgccatgttg atcccggatc caatgactat ttgcgccgca tcgccagttt
     2641 tgccagtcag ctccacaaga atgcctgcca gcttgcgatc ctgcagatag aggtcattag
     2701 gccatttgac acgaacttta tctgctccca gctttcgtaa tacttccgcc atcacgatac
     2761 cgataaccag acttaaacca atcgccgccg ccgggccttg ttccagacgc cagaacatcg
     2821 acaaatataa gtttgcgcca aaaggcgaaa accatttccg cccccggcga ccacggccag
     2881 cctgctggta ttctgcaaca caggcatcgc ccgatttaag ctctccgata cgatcaagaa
     2941 ggaactgatt cgtggagtca ataactggca gcacggctac actaccgcca tccagctgac
     3001 ccaatatctg ttcagcatta agtaactgga tgggctcagg caggctgtat cctttacccg
     3061 gaacggtaaa gacatcaacg ccccagtcac gcagtgtctg aatgtgttta ttaatagccg
     3121 cccggctcat tcccagcgtt tcacccaact gctcgccaga gtgaaattca ccgttcgcta
     3181 acagggcaat caatttcagt ggcacggtgt tatccttcat gaaattgtct ccactgcgct
     3241 cacttcacct gatgcaccaa taaagcggac ttcaggctca agccagacat taaatttttc
     3301 acccactttt tgtcttacat ggtgtgccag ttgcaccaca tcttcgcttt ttgcattgtc
     3361 ttcattaatg agaactaacg cctgttgacg gtgcaccgca gccccaccca tttgcatccc
     3421 ttttagctgg cactgatcaa taagccaacc tgctgccagt tttactgaac cacccgcctg
     3481 ggggtaattt ggtgctgttg gaaattgcgc cagtaatgct ttagccgttt cggcagatac
     3541 aacagggttt ttgaagaaac taccggcatt gccattcact tttggatcag ggagtttggt
     3601 ggtgcgcata tgacacaccg cattaaatac ttgctgtggc gttactgttg taggatccag
     3661 acgagttaag tcaccatacg ttagtacagg ttgccactct tttggcagac gcagacctac
     3721 ggctacaatg gcgaagcgat cctggtattc atgtttaaaa atactgtcgc gatagccaaa
     3781 acggcactct ttggcagtta agcgcacttg cttgcctgtc gccagttcaa cacaatcaac
     3841 ataagcgcaa actcgctgta attctacgcc ataagcacca atattctgga taggtgatga
     3901 gccgacacaa ccaggaatta atgccagatt ttccagacca ggcatacctt cctgcaaagt
     3961 gtattttacc aggcgatgcc agttttctcc ggctcctaca tgtaaatacc acgcatcagg
     4021 ttcatcatga atttcgatac ctttgatccg gttgatgatc accgtgccgc gatagtcttc
     4081 cagaaaaagt acattacttc cttcacccag aataagaacg ggttgtcctt ctgcggttgc
     4141 atgctgccag gcattgagta attgttgttc gtcttcggca catacaatgt gctgagcatt
     4201 atgatcaatg ccaaatgtgt tccagggttt taaggagtgg ttcatagctg ctttcctgat
     4261 gcaaaaacga ggctagttta ccgtatctgt ggggggatgg cttgtagata tgacgacagg
     4321 aagagtttgt ag
//
LOCUS       DAJTYG010000194         4305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTYG010000194 DAJTYG010000000
VERSION     DAJTYG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.196"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..157)
                     /locus_tag="OO568_003943"
     CDS             complement(<1..157)
                     /locus_tag="OO568_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643050.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASK"
     gene            complement(402..932)
                     /gene="cybB"
                     /locus_tag="OO568_003944"
     CDS             complement(402..932)
                     /gene="cybB"
                     /locus_tag="OO568_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6643051.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1121..2122
                     /gene="gap"
                     /locus_tag="OO568_003945"
     CDS             1121..2122
                     /gene="gap"
                     /locus_tag="OO568_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6643052.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATKTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="OO568_003946"
     CDS             complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="OO568_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6643053.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3800..>4305)
                     /locus_tag="OO568_003947"
     CDS             complement(3800..>4305)
                     /locus_tag="OO568_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6643054.1"
                     /translation="TIQVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITLLNQAVER
                     VHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQG
                     LWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEA
                     MESRSLR"
BASE COUNT         1142 a         1076 c          987 g         1100 t
ORIGIN      
        1 ttttcgatgc actaatagaa ccatcattac agacaaattt gccgcctgcc gtacagtgtg
       61 agatcccccc tttcgaacca gaacagggtt tgcgcccggc atcggcataa aaggaaacaa
      121 gcgttcccat aaacaaaata agtgccagtt ttttcatata acctccatgg tttatcgaat
      181 cttatgtgcg atttgaacgt tatacgctgg tttattttga taccgttgat gagatcaaag
      241 atcgggtgaa ttgattaaaa ataagccaga tggacgtatg ggcaaggatt atggttttat
      301 ttgtcataca aataaatatg ataggcgctt ccgatgtaga cccgtattct tcgcctgtac
      361 cacgggtcgg tttttgtaca ggcgttttct ttaaatatcc ttcaggaacg tttacgcggc
      421 atcatgcgta gaagtgtgtt gtccttccag aaatagtggt gtgccagtgc agccgcagcg
      481 tgcaacccga tgacaaaata tcccagattc gccagcgtct catgccacga ctttaagcta
      541 tcaacccgtt cgaaattggc ctctgaagcg taaggcatcg tcaaaccaaa cgcaaaccac
      601 gggttgcccc ggttatacat catcaccaaa ccaatcactg gcagcgcaat aaacaggaga
      661 taaatcacca aatgtcccaa atgcgccagt cccgtcatca tcggtttcgg cttaggtata
      721 atcggcgggg ttgggtattt cagtcttaac aaaagacgaa ccaccatcag cacgagaatt
      781 gagatgccac aggaaacatg aatcatgttg atgagcggcc gatcgctacg tgggaagaaa
      841 ccacgaaact ccattgcgca atacgctgcg ataaccagca aaaagaccag ccagtgaatg
      901 ctgatttgta accttgagta tttattttcc ataacatttc ctgctttaac ataattttcc
      961 gttaacataa cgggcttttc tcaaaatttc attaaatatt gttcacccgt tttcagtacg
     1021 ttacgtttca ctgtaagaat ggagattgcc catcccacaa tcctggtcta agcctggaaa
     1081 ggatcaattt tcatccgaac gttcctgaca ggagaaaacc atgagtaaag ttggtattaa
     1141 cggttttggt cgtatcggtc gactggtgtt gcgtcgttta cttgaagtca aaagcaacat
     1201 agacgttgtc gctattaatg atcttacttc cccaaaaatt ctcgcctatc tgctgaaaca
     1261 tgattcaaac tatggtccgt tcccctggag cgttgatttt acggaagatt cacttatcgt
     1321 tgatgggaaa agtatcgcgg tttacgccga aaaagaggct aaaaatattc cgtggaaagc
     1381 gaaaggggca gaaatcattg tcgaatgtac tggcttttat acctccgccg ataaatcgca
     1441 ggcgcatctt gatgctggtg cgaagaaggt gttgatttcc gctcctgccg gtgaaatgaa
     1501 aaccatcgtt tataacgtta atgacgacac tctggatggc aacgacacca ttgtttccgt
     1561 ggcgtcatgc accactaact gccttgcgcc gatggccaaa gccttgcatg acagtttcgg
     1621 gatagaagtc ggcacgatga cgaccattca tgcctatact ggcacccagt cactggtgga
     1681 tggcccgcgt ggtaaagatt tacgtgcttc acgcgcagcg gcagaaaata tcattcccca
     1741 cactacgggg gcggcaaaag ccattggtct ggtgatcccg gaactgagcg gcaaactgaa
     1801 aggtcatgcg caacgcgtgc cggtgaaaac aggttcggtc actgagctgg tgtccattct
     1861 cggaaaaaaa gtgactgccg aagaggtgaa taacgcactt aaacaagcaa ccaccaataa
     1921 cgagtcattt ggttataccg atgaagaaat agtctcttcc gatatcattg gcagccattt
     1981 cggttcagtg tttgatgcca cgaaaacgga aattaccgcc gtgggcgatt tacaactggt
     2041 gaaaacggtc gcctggtacg ataacgaata tggcttcgtc acgcagctta ttcgcaccct
     2101 cgaaaaattc gctaaactct gacgcgcaca ggcggaggaa aaaacctccg cctctttcac
     2161 tcattaagac tgtaaataaa ccacctgggt ctgcagatat tcatgcaggc catgtttacc
     2221 atctgcgccg ccaataccgg atttacgcca tccggcgtgg aaaccttgca tagcttcgaa
     2281 gttttcacgg ttgatgtaag tttcaccaaa cttcagccct ttaatggctt tcatcgcgac
     2341 gttcagattt tgggtataga ttgatgaggt caggccgtaa tcactgtcat tagccattga
     2401 gatagcatct tccagcgtgt caaatgcgac aactggcagc accgggccaa aggtttcctc
     2461 atgcataatc gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc
     2521 tttcccctct accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc
     2581 cactttttgc tcaacccttt ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc
     2641 gttgcgttca gcggggttac caaattgaac cgcctgcaac gcttcaccca gccgattgac
     2701 gaactgatca taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc
     2761 actattaatg acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc
     2821 cattacgata gccggtgctt taccacccaa ttccagacac actttggtga tgtttttcgc
     2881 cgcagtcgcc atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac
     2941 ctttgggtta cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa
     3001 cacgccgcgc ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg
     3061 cgtaaattca ctgggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt
     3121 gcgggcaatg aggaagaacg gaaagttcca cggcagaatg ccggtagtca caccaagcgc
     3181 acgtttaaac agaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta
     3241 acgccgtgcc cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc
     3301 agctagctgc tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg
     3361 ttcgcggatc ccggcggaga ttttgcacaa ccaactggcg cgttcaatag caggcaacgc
     3421 ttcccattct ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg
     3481 accatcgggt atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc
     3541 gtctccacgc caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga
     3601 catgggcgac tcctgtgatt tatatgtttg ttttcggtga atatattgtt aattaacatc
     3661 aatcatcaga gagacggcat cgggaaagga caattctgtg gagggcttca taaaaacaag
     3721 tgtaaaagga ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg
     3781 gttcacaaac gggcaatttt taacgtaacg agcgactttc catcgcctcg atgagcaccg
     3841 catcatgttt cagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga
     3901 taaaatctcg cccgcgggga ccgtagccat cgctatcatc gtgtaaacgc ggcagctcgc
     3961 cagtgagtag tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga
     4021 ttacactgtc tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg
     4081 gtgcatcggg attgtccccg gtcatacggc ggaacgttgc catcgtgcgc cgctgcatgg
     4141 tggggtcctg tacaacgata gccgtatgaa ctcgttctac ggcctgattc aatagcgtga
     4201 tgctaaagcg tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat
     4261 gcggaatatg ccagaactga tgagcgatat ccacctggat ggtcg
//
LOCUS       DAJTYG010000195         4187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTYG010000195 DAJTYG010000000
VERSION     DAJTYG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.197"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..148)
                     /locus_tag="OO568_003948"
     CDS             complement(<1..148)
                     /locus_tag="OO568_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCR6643055.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGL"
     gene            complement(298..459)
                     /locus_tag="OO568_003949"
     CDS             complement(298..459)
                     /locus_tag="OO568_003949"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643056.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(456..644)
                     /locus_tag="OO568_003950"
     CDS             complement(456..644)
                     /locus_tag="OO568_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6643057.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            904..1239
                     /gene="iraM"
                     /locus_tag="OO568_003951"
     CDS             904..1239
                     /gene="iraM"
                     /locus_tag="OO568_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6643058.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1520..1651)
                     /locus_tag="OO568_003952"
     CDS             complement(1520..1651)
                     /locus_tag="OO568_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCR6643059.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2151..2227)
                     /locus_tag="OO568_003953"
     tRNA            complement(2151..2227)
                     /locus_tag="OO568_003953"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2191..2193),aa:Arg,seq:tct)
     gene            complement(2329..2404)
                     /locus_tag="OO568_003954"
     tRNA            complement(2329..2404)
                     /locus_tag="OO568_003954"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2369..2371),aa:Ile,seq:cat)
     gene            complement(2548..3369)
                     /locus_tag="OO568_003955"
     CDS             complement(2548..3369)
                     /locus_tag="OO568_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6643060.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3384..3740)
                     /locus_tag="OO568_003956"
     CDS             complement(3384..3740)
                     /locus_tag="OO568_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6643061.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3753..>4187)
                     /locus_tag="OO568_003957"
     CDS             complement(3753..>4187)
                     /locus_tag="OO568_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6643062.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPES
                     PESFMKRPKRRSWVNEKYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTL
                     PLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1154 a         1134 c          824 g         1075 t
ORIGIN      
        1 gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac ggggaaaacc
       61 tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg tatgacacac
      121 ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga agctgacaac
      181 aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag cgtctcagac
      241 gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc tcacttttta
      301 tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc catacgttgc
      361 atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac tgtcttttat
      421 ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt tgcaccactg
      481 accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt tatggccacc
      541 ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc cgcaagatct
      601 gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct ttccaccatc
      661 tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa aaccatacct
      721 taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac gagatataaa
      781 ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgc tctatggttt
      841 aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa acaaggaaac
      901 taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat atctttttca
      961 gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt cttacctcct
     1021 gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt gctacctgta
     1081 accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa aaacagccag
     1141 gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg ttgcactctg
     1201 caaatatgtc cttatggact aaaacaacaa agtccataag gagtttactc acatctgaca
     1261 aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg tgccattgca
     1321 tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga ttatcccata
     1381 tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt tcttttaaca
     1441 aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt aaaaatttct
     1501 cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac gggaaagcaa
     1561 caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc cggctgctgc
     1621 aatgccattc acaaaaacag taagcaatca ctttttaccg taacaggtga taatccagat
     1681 atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc tggctattct
     1741 gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact catcgcaaac
     1801 cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac gatcaggagt
     1861 acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga ctatatcaat
     1921 acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac ccacaccata
     1981 tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat actcaatcaa
     2041 tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata tcctgagcca
     2101 ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata tggcgcgctg
     2161 tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc ggctgagcta
     2221 acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca ccaacaaccc
     2281 tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg gtggcccttg
     2341 ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac tgagctaaag
     2401 ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaac ccgctcagcg
     2461 gcgggttgta gaaactcttc taacgtcagg cataaaacgc ccatcattat gacgaattta
     2521 ccacagattc cggaaaaatc aaccttgtta cctagttacc ttttttaact gccgctcagc
     2581 ccatgcttct tcaatatcaa accgggtcac cagcgcatca taaaatttct taactgtttt
     2641 ttcccatgac gcgcgtgtta tctggtttgt cacctcgcat atagcattaa atgcctccgt
     2701 tgatggtagt ctttcatagc cacgaccacc acaacgctgg cagtctctga taacaggcat
     2761 accacgtttt accgactctt cacgatgaat ggcgacacca cgcccacggc aatccttaca
     2821 ggcggtggaa acctcaccct ttccgccaca ctccggacag gcaactttta ccacctccct
     2881 gacttttttc cattcttccc agtaagacgg atacacacct ttcgtgcact ttgcccatac
     2941 cggcggctta ccatccggat actggatctt gtttgtaaaa actatgcttt caataaattt
     3001 ttccccatag caacaagggc actgcttttt actcgctgcg ctgcgggcat aatcctcaaa
     3061 agcgtacgaa gccataatgc gcatcactac cggttttatt tctgccggaa gttttctcaa
     3121 cgccgccaca cgatcgcacc gactgagtgc ataatctgcc agtaattctg ttgcccgcgc
     3181 cctgtcattc atactgatgc ccattttccc caggaacgca gaaaaaccca tctcagcccg
     3241 attctgtgtc atgccctgcg cggccatcac atcagtgata ctcagcgcat cttttgacgt
     3301 tgaggccgat gcatcggtca ggccagggga ttttggggag tagtatttcg gtaaatcttc
     3361 cagtttcatt ttttgacctg ctcttaatgc attatggggt aaatcttcac ccccagacgt
     3421 ccaccagata ctggctgacc acgaacgata ttgatttcat caaactgctc atcgtccatt
     3481 aacactcccg catgcgtcag cgcatccagc ggtgctttca ggatattgtc caggtcgcga
     3541 cgacgcttat ccggtggctc tgcaatcacc tttatcgcca gccttccgga caggcttaat
     3601 ttcagccgct gctggcgaac aataagcgcc acagcccggc gataacgctt tccctcctcc
     3661 gagataaaat atgtgctgcc acggcgtcgc cagtaagtgt tcaccgtcgg cgggtaaggt
     3721 aaaaccaaat ctatgagcat cagtcacctc ttttacccaa gcacgccagt tgcaaaggcg
     3781 tgatcaagaa aacgaaaaat taaatcaacc tgagaaccat gcttttcttc gaacgccaga
     3841 ggatccgcat gaagctcgtt gtgatgctcc cgacacagcg gtagcgtgaa aatatcgtga
     3901 gattttgtcc ccattccgcc ctgaccatga ccaatcaggt gatggggatc gtcggctggc
     3961 ttaccacaac acgcacacgg ctgtgtcttc acccagcgtt tgtatttctc gttaacccag
     4021 ctgcgacgtt taggtcgttt catgaaagat tccggagact caggatcaac ggcaatgctg
     4081 accaccgtct tttcctgttg tgggttctgt tgctggtggg cgtgaggcag cggcgcaaga
     4141 ttttttgtgc gctgtttcag tatgctggtg gcggtctgct ctcccgg
//
LOCUS       DAJTYG010000196         4111 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTYG010000196 DAJTYG010000000
VERSION     DAJTYG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.198"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            223..1196
                     /locus_tag="OO568_003958"
                     /pseudo
     CDS             223..1196
                     /locus_tag="OO568_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1193..2098
                     /locus_tag="OO568_003959"
     CDS             1193..2098
                     /locus_tag="OO568_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCR6643063.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            2095..3165
                     /locus_tag="OO568_003960"
     CDS             2095..3165
                     /locus_tag="OO568_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR6643064.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECDTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVL
                     QKSLEITEEADLALRNAMARIK"
     gene            3301..3978
                     /locus_tag="OO568_003961"
     CDS             3301..3978
                     /locus_tag="OO568_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643065.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGK
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
BASE COUNT         1069 a          968 c         1139 g          935 t
ORIGIN      
        1 cttatcgatc aactgataac ggaagtcata ctgagttcac actccagagc tgcggcactg
       61 gcggttgatt ctgccgccgc aaaactgatg cagaatgcag acaatatcag tgaatacctg
      121 tcgttacacc atcaggggct gatgcaaagt attcagtcac tgcaggcgca tatcaccagc
      181 ctgcttgagg atatccggga aatagcagac tgtcaggagc aggtgaccgc tgatgtcaga
      241 atggccatgg aggagatcga tgccagaacc cgggaattac tgacggggtc tgcacctcac
      301 tggaagagga gctgaatgac tatttcagaa gcggtaaacg caaagaacag caaatgctgg
      361 aggaagaaaa ttcagcgcag ccccgggaga gaaacgcatt cgctttcttt cacgatattt
      421 tcggaaccgg aaatcagcat gacaggatgc gggactttga tccggacagc cccgaaataa
      481 aattcagtga tcgcagggaa gcccttgaac tgatgacgca aatcgaatcg accgtgacca
      541 gcctgcaccg agaggctgaa gcacagttcc ggcctgagct ggagaaaatc gtcagcggga
      601 ttgaaacagg ttttcgtggc acggccctgt acgccacaga aaacattgcc ggtcgcatca
      661 atacccgcct ggaggatgag ggctttaccg taaaaatcag ttttccggca gtcagtcagt
      721 tacaaacccg gctcgcggta aaaacaaatc tgagtgcgct tatggaggaa agaacggaga
      781 ccgtcacccg tcgccgtcgg cagcgtggtt tatggggaaa gatttgtgga gcgtttggca
      841 ccagtgactg gggctgggaa acctataaag aggatgtgag ccgcagtgtt atcaatatca
      901 acaccgtcag gaaggaagtt atgtcactga cccgggcata tttcggggag ctgcaggcat
      961 ccattgagca ggatattaac cagcccgtcc gccaggaaat cgatgccttt ttctgcgcat
     1021 tcagggagaa agttgaacaa ctgcgtaaca cgctgattca gagctctgaa gatcataaac
     1081 gcgatcagca ggcacaggaa cggcttacca ggcgacttca ggcattaaat gaaagggttc
     1141 ctgagctcat tactgacagt aaggcgctga gggaagagct ggagacaatg ctgtgacttc
     1201 accatttatt caacaaattg ctgataaccg ggtatgtcag gtactcacct gtcttcctga
     1261 aaaatttgtg gttgatttcg caaacggaat cgatgttgca caggagcata tccgcacggc
     1321 cggagagcgc acgttcttcc ggcgtttaaa agagggactg accggcgaag gcgctgcacg
     1381 gcagaatgcc atcaatgctt cggttgcaca gggcgttgag gcgtccctgc gctggctgac
     1441 ggagctgaca acgtcacttg ccaccacaaa ctacgcgatt acccgggtaa acgacagggt
     1501 cagttcactg gtcagtgata ctgccaggtt ggcgcattat tccgcagata cacgggagca
     1561 gttactcatc ctggccgatc aggttcacca taaactgaat catcttgaag aaaaactcca
     1621 ccgtgttgac caggttcagc gggcgcagtt acatcttgag cagatattct catggtggag
     1681 cgccgggcga tacgcgtcat tttcccctgc cggacgttgt tatgtggcac ttgaagagct
     1741 tcgctggggc gcgtttggtg atgtgatacg tcagggcgaa acaggccagg ttaaccagct
     1801 actggatatt ctcagacata aagcattaac gcagatggca caggagagtg gcggtagtgc
     1861 aaccgtgcgt ctgaacactc tggactggct tggtggtcag ggccgggagc aggcagataa
     1921 cgagtggcat gacgcgatta actggctggg agactggtgc agtgaagagc agcatccggt
     1981 gatctggtca accacgcagg ctgcagaaca cttaccggtt cgcatgcctc gcctctgctc
     2041 tgcagaacgc ctctctgaaa gtatggtcga tgaaatattt cagaaggggg ctgcatgagt
     2101 acagaaatga aaacggggct ggtgctgtcc ggtggcgggg cggtgggcgc ttatcaggcg
     2161 ggagtggtta aggcactggc agagtgtgat acacagatca gcatggtttc aggggccagc
     2221 attggcgcat tcaatggtgc cattatcgcg gcctctcccg atctgtcaga agctgccgta
     2281 cgcctggaag cgctctggga tcatctgggg aataatcagg tgctgtcggt aaaccgattg
     2341 gtttactttt cattgctgaa aaaattgttc caggcaatga acctctgcca gatccccgga
     2401 cgtgcaggag cactgcttac gacgcttctt cgccatatat cgatactcaa cgggtttgac
     2461 aatctgatgg ctcagccgtt gttgtcagat gagcccctga cagcgctgtt ggatcattat
     2521 cttgatactg atgctctggc agacgggcta ccgctgtatg tgtcgctgta ccccacagaa
     2581 gggggcatgc aggatattat tgactgcatt cgtgctgaac tgggtgccgg aaccacgaaa
     2641 aacgccgttt ttcagcatat ccagagcctg ccccgcggac agcagaaaga ggctctgctt
     2701 gcgtcagccg cgctgcccct gctgttccgt ccccgtgagg ttcaggggac aatgttcggt
     2761 gatggtggta tgggaggatg gcgaaatatg cagggaaata cccctgtgac gcctctggtc
     2821 gatgccggat gcaatatggt gattgtgacg catctgagtg acggttcttt atgggatcgc
     2881 caggcttttc cggacaccac aatccttgag atccgtcccc ggaaaaggct gaaatatgca
     2941 ggtgatggcg acaacagcgg cggtctgctc agtttcacgt tggcacatac cgacgcctgg
     3001 cgtcagcagg gctatgaaga cacgatgctg acgatggagc atatccggaa accgctggca
     3061 gcacgtcagg tactgtcccg gtcagaaacg gtgttgcaga aaagtctgga gataacggaa
     3121 gaggcagatt tggcactgag aaacgcgatg gcccggatta aataaagacg ctccaaagaa
     3181 acgccaccgc ctgtggtggt gtttttccta gctggtgtta ttgaaaatat tttcagatat
     3241 atattacctg agagaaagtt ttacccctca cctgtcaatt cactcactgg aaatttaatt
     3301 atgcaattac ctgtaaagtt attaatgagc cttatatctc tggtcagcgt tattgcacgt
     3361 gccgggaaat ataaaaatta tatccgggat gaaataaaat actggcgata tacatcatac
     3421 aagggtggga aatttccgga aggtttcact gatgagaaat tttccagcgc catttacaac
     3481 ggaagaatat ttacaatgaa acgtttacat accctgatgt tatttctggc ggttctgttt
     3541 actggcttta acgtggaagc agcgagcgtg aaacaagcgc tcagctgcga cccaaacgcc
     3601 cgggctgaac aacctggagc gtgtccaaca acgtacgagt tgtacgaagg tgacgctgcc
     3661 tacaaagctg cgcttgacaa agcattaaaa ccggtcggac tgagcggcat gttcggtaaa
     3721 ggcgggtata tggatggccc tggcggaaac gtaacgccag taaccattaa cggtacagtc
     3781 tggctccagg gcgacggttg caaagccaat acctgcggct gggactttat cgtaacactc
     3841 tataacccaa aaacccatga agtcgttggc taccgctact ttggtttaga tgacccggcc
     3901 tacctggttt ggttcggcga aattggcgtg catgaattcg cgtatctggt gaaaaactac
     3961 gtagctgcgg ttaactaatt taaggagtct gtcatgaaaa cttttatcaa aactttactc
     4021 gttgctgtaa ctattctgtt ctctgtcttc gctacggcga aacaagtaaa actgccaaac
     4081 aacatcaaat acgttaatac tacagaggcg t
//
LOCUS       DAJTYG010000197         4078 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTYG010000197 DAJTYG010000000
VERSION     DAJTYG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.199"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..273)
                     /locus_tag="OO568_003962"
     CDS             complement(<1..273)
                     /locus_tag="OO568_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR6643066.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
     gene            complement(286..2067)
                     /gene="gcl"
                     /locus_tag="OO568_003963"
     CDS             complement(286..2067)
                     /gene="gcl"
                     /locus_tag="OO568_003963"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR6643067.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGIINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2157..2972)
                     /gene="allR"
                     /locus_tag="OO568_003964"
     CDS             complement(2157..2972)
                     /gene="allR"
                     /locus_tag="OO568_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR6643068.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3050..3532)
                     /gene="allA"
                     /locus_tag="OO568_003965"
     CDS             complement(3050..3532)
                     /gene="allA"
                     /locus_tag="OO568_003965"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR6643069.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3762..>4078
                     /locus_tag="OO568_003966"
     CDS             3762..>4078
                     /locus_tag="OO568_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6643070.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNP"
BASE COUNT         1064 a         1119 c          942 g          953 t
ORIGIN      
        1 aatcgctgct gctacgccat cccgaaactc ttcttcacgg ccaggaatac aggcaatacc
       61 gcgctcgccc gccgcccagt cacctgccgg taaattgtgc agcgtatgtt cgagtttatt
      121 actcgccagt acctgtttta attcttcaat gtcgtagtca taaggaaaca taaattcaac
      181 gccgcgaaaa ccacactgcg cagctttctc aaaacgggcg agaaaatcat attcaccaaa
      241 taacatcgat aaattagcag agaaacgtaa catcatttat ctcccttatt catagtgcat
      301 gaagcaggtt tcagtcggtg catccgctgc gttatcggcg atatcttcaa attccatgac
      361 gttatccagt tcgctgccca tcgaaatatt ggtcacacgc tcgagaataa cttccacgac
      421 taccggtacc cgatattgcg ccattaaggc tttcgcctgt tcaaaggctg gcgcaatatc
      481 ttccggtttg aagacccgaa tagctttaca acctaaacct tccgctactt ttacgtggtc
      541 aacaccgtag ccattcactt cactggagtt gatattctcg aaagcgagtt gcacgcagta
      601 gtccatgtca aaagcgcgtt gtgactgacg aatcaggccg agataggcgt tattcaccag
      661 cacatggatg tacggaatgt tgaactgcgc gccaacggct aactcttcaa tcaggaactg
      721 gaagtcaaag tcgccagaaa tcgccaccac attgcgtttc ggatcagcgg cacaaacccc
      781 cagcgccgcc ggaatcgtcc agcctaacgg accagcctga ccacagttga tccagtggcg
      841 gtctttaaag acatgcagca tttgtgccgc agcgatttgt gacagaccaa tagtggtgac
      901 ataacaaaca tcgcgaccaa aggctttgtt catctcttca tacacgcgct gcggtttcac
      961 cggcacgttg tcgaagtggg ttttgcgcag caaagtgcgt ttacgctgct ggcagtcggc
     1021 gacccattct ttacgacacg gcagacgacc cgctttttgc atctcctgcg ccacttcaac
     1081 cagcagtgtc agcgccgctt tagcatcaga gacaatgccg agatccggac acagcacgcg
     1141 accaatttgc gtcggctcaa tatcaatatg aacgattttg cgcccttcgg tgtatttctc
     1201 taccgaaccg gtatgacggt tagcaaaacg gttaccgata ccaaacacca tgtcggacgc
     1261 cagcaacgtt gcgttaccgt aacgatgcgc ggtttgcaga ccgaccatcc cggccatcag
     1321 ttcatgatcg tccgggatac agccccagcc catcagcgtt gggatcaccg gaacgctggt
     1381 cagttcagca aactgttgta acagtgcagc tgcatcagca ttgattatcc cgcccccggc
     1441 aacaatcacc ggacgttcgg cctggattaa catttctaca gctttttcga tctgcatacg
     1501 gctggcagca ggtttgtaga ccggcagcgg ttcgtacatg tcaggatcaa actcgatttc
     1561 cgcaacctga acgtcgaacg gtaaatccac cagtaccgga cccggacgac cagaacgcat
     1621 caggtgaaat gcctgttgca gcacgcgagg caccagcgcc gcttcacgaa ctgtaaccgc
     1681 cattttgctg accggtttag caattgcttc aatatctacg gcctgaaaat cttctttatg
     1741 cagacgggcg cgcggtgcct ggccggtaat gcacagaata ggaatggaat cagcagaagc
     1801 ggaatagagc gcggtgatca tgtccgtgcc cgcaggaccg gaagtcccca gacatacgcc
     1861 gatattccct gccgttgcgc gggtataacc ttccgccatg tgcgaaacac cttccacatg
     1921 acgcgccaga atgtgacgaa taccgccgtg cttacgcatc gctgagtaga acggattaat
     1981 tgcagctccc ggaacgccga aggcggtggt gataccttct ttctccagca catacattgc
     2041 cgcgtcaacg gctctcattt ttgccatttt attcctacct ctattaattg gaaaaatttt
     2101 ccaactttta aattttgtca aaaaaacccg ctccgcagag cgggatgcga cagacactat
     2161 ggatgtgctt tcagtcccaa cgccgtgctg atatcgcggg cggtgtctct gaccagctca
     2221 ccctgactga caaaacgatc ttctgtcagt cttgatgaag gcccggagat ggagatagcg
     2281 gcaacaacgc taccgacatc atcgtaaatt gctgaagcta tgcaattcag acctacaaca
     2341 tgctcttctt tatctacggt atagcccagt tcacgcgctt gttccaggtc cttcagcaag
     2401 gtgggcatat ccacaagcgt agttggcgta aactgctgca aaccggtttg cagaatgatg
     2461 ctcatcaact cctcttccgc cagcggataa agcagcgctt tgcccgcacc ggaagcatgc
     2521 agcggcagac gactgcccag tggcgcacac atcctgacca tcgatttaca ctctaactga
     2581 ccaattaata ccgcttcatt gccgttacgg atcgcgacat tgaccgtttc gccggaaagt
     2641 aacatcaggc ggcgcataaa cggcccggcg acggagagga catcgcggtt atggatgtac
     2701 gccgcaccga cgttaaagac acctaatcct atatgccacc agcctaattg actgtcctga
     2761 tagacaaaat ccgctgcttg taaaaccttc agcaagcgaa aggtcgtgga gagcggcaaa
     2821 tccagattga gagaaatatc gctaaccgac gaacttcccc cacttttttc cagatattgc
     2881 agaatcgcaa ttccccgctc taacgcctgt gcgcccttct gtgccacagg ctccgcctgt
     2941 cctggcctac cgcgccgtct aacttccgtc atacctacgt taactccgac ctacaagtga
     3001 gcataaacaa tgaacaatta gtttatctta tgcaagtcgg ttgcaggcgt cacgcaaaac
     3061 agagttcctg ttcaggaata ctttcaacat cacagttgtc actgccgccg cgatcgatgg
     3121 tcagaaaatc ggtgacgcgc tgccaggcga aaagtgggtg atgccagacg ttacgatggt
     3181 aattcacccc ctgttcaccg ttggtgataa acgcccgcag cgttgacagg tctggtttgt
     3241 cgtcgcctaa cgccacgacc accacaaaca cttcaccttt cattgggata aaggcctgag
     3301 tgcccagcgg atgacgttcg agttcgtgaa tggtcagcgg cagattcgcc ggttgcgcgc
     3361 ggttaatgct gataagcgta cggtcttgct caagaatctc aaccagcgcc aaatcgtggt
     3421 aacgctccac caggccattg ttaatatgga aaaaatcccg ttgctgcgtt tcgattacgt
     3481 cgccataagc actaaaggct tcctgactta acggtaatac ctgaagtttc atctttttgt
     3541 ccttgtaaca taattccgtt caaccaccgc cgaccggtca gcttggtttc aataacaggc
     3601 aatctaaaac cttaagagaa caacatcaat acaaaagttg gaatgttttt ccaaaactag
     3661 atttagtcca caaattgaac aggattatca tgtaaaaata ctgataaatc agaggataag
     3721 aaaatcacgc aacttgcgga tttaaaaggg gatacctgaa gatgttcgat ccagaaacct
     3781 tgcggacttt cattgcggtt gctgaaacag gaagtttttc aaaagcggca gaacgattat
     3841 gtaaaaccac ggcgacgatc agttatcgca ttaaacttct ggaagagaat accggagtag
     3901 cgctgttttt ccgtacgact cgtagcgtaa cgttgacagc ggctggcgag catctacttt
     3961 cccaggccag agactggctg agctggctgg aaagtatgcc cagcgagctg caacaggtga
     4021 atgatggcgt ggaacgccag gtgaatattg tcatcaacaa cctgctctat aaccccca
//
LOCUS       DAJTYG010000198         4025 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTYG010000198 DAJTYG010000000
VERSION     DAJTYG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.200"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..305)
                     /locus_tag="OO568_003967"
     CDS             complement(<1..305)
                     /locus_tag="OO568_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6643071.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
     gene            complement(516..1913)
                     /gene="ccp"
                     /locus_tag="OO568_003968"
     CDS             complement(516..1913)
                     /gene="ccp"
                     /locus_tag="OO568_003968"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6643072.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLVLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2318..3967
                     /locus_tag="OO568_003969"
     CDS             2318..3967
                     /locus_tag="OO568_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6643073.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1042 a         1013 c          940 g         1030 t
ORIGIN      
        1 tttacgcaac gcaggtcgat ggctgcggat tgcggatatt cttctttgtc gatccagttt
       61 ttattgatcg acaaatccat caatttatgg acgttttcgt cgtcccaggt ctggcagaaa
      121 gtggctaggt tctgacgagc gttgccatca agatataatt catcattgat aatctggaat
      181 gcgacatcat cgcgcatttc gtgcagcgga aatcgttttg actccgcgat agtagaaatg
      241 gcctttgcgc caaaacgtga atcgagtagt tctgagcgga aatccgttaa cagcttctgg
      301 tccatttcga actccttaaa tttatttgaa ggcaataaaa aagtaggatt tatccgcaat
      361 ggaagcaagg cattacaggc ttaatttaaa taacaaaatc ctaagcagaa aaacgacttt
      421 ttataatata aacatttaac atgataatat taaaaacaga ctttattatc gtaataaaaa
      481 tcgcccaaac attcctgttc gggcgatcaa attaattatt gtttatcctg catatacggc
      541 gtgtacaccc cgttcagact gtgcaggaaa gctacgatat catccacatc ctcctgcggc
      601 agctctttgc ctacctgata gcgcagcatc agtttcaccg ccccgtccag cgtcggcacg
      661 tcaccgcgat ggaagtacgg tgccgttaaa gcaacgttac gtaaaccggg tactttctga
      721 cgcaatttat cacgctcttc tttagtcaca ttcatacgac caatatccgc cgccgtaatt
      781 tccccaaagt taaagtcttt tttcagcacc aacggttcaa aggaacgtcc gccgagaata
      841 ataccaccat gacaagttgc acatttatta tctttaaata attgatagcc ttttttctgt
      901 tgcgccgtca gcgcattttc atctccacgc aaccatttat caaatgggga atccggcgta
      961 attaatgttt tctcaaattc agcaatggca tcagtaatat tttcgccact gaaaccttgc
     1021 ggataaactt cgaggaactg cgctttaagc tgcggatctt tttccagctt agcaataatt
     1081 tcgtcccagg atttcgacgc catttcaatc gggttcaacg gcggtccacc tgcctgatcc
     1141 tgcaatgttg ccgcacgacc atcccagaac tgctcaacgt taaatactga gttaaatacc
     1201 gtcggcgcat taatcggccc aactgcgcca ccaacaccaa tcgatgtttt tctgccatcg
     1261 acgccccccg cattcaacgt atggcaatga gcgcatgaaa tggtgctatc agccgataaa
     1321 cggggatcgt gatacagcgc aaagcccaac gccacttttt gcgcatcggt aggcagtttt
     1381 tgcgggatgg gctgcaccgg ttcattgcga tgctccggag cagtatcatt gctggcgtaa
     1441 tattccgcgc gctgttttgc aatccaggcc agtatttccg cccgctcttc atcactcacc
     1501 ttacccgccc agtgtagcgc ggtataacgc gttggtggca tagtttcata ctgcatcacc
     1561 cattcaatct tattcagatc gctttgcgaa acgggtttat cagccagcag tgccgcacgc
     1621 acggcctcga ggttaaaaga tttatatcca agcttaatgt cgtaatccat caactgtttc
     1681 gcgccaggaa tataataata ggcgggtaat tctgccgaag gcgtgtggca atagtcgcat
     1741 cctttttcgc ggagaaagcc taaaacctta ttattttcac tgacagcaga tgcctgaaca
     1801 tcggcctgtt tactgcgttt attatcgtgg taccacacat aaccagataa ccctaaatag
     1861 caaatcgcga cgccagccag gccgatcgcg gtaatacgtg agaccatttt cattattctt
     1921 ccctcacggt ggtgagttat tgtgacccag aaaattttcc agaatgctgc aaacgtctct
     1981 gcgagacaaa gccatcataa aaaacaggga gatacgcttt ttgataggaa tcaatcaatt
     2041 acatgaatat aaactatcaa taagatagcc tgtgcctatt acccagatca ggaatggcat
     2101 atcatgattg cggcgagagc aggattgaat gttctgaaaa tgaaaaacaa caccaaatcc
     2161 gtatttttat aaattccatt gatattagtg cgttatattt tttgacaagt taataaacgt
     2221 gatccaccgc acgctttgtc gcccaccagg cggagcgaat gactaccctt aaagaaaagc
     2281 ccgataatta gcgacgaatt tcggaggttg gatccttatg ctcaatcaga aaattcaaaa
     2341 ccctaatcca gacgaactga tgatcgaggt cgatctctgc tatgaactgg acccgtatga
     2401 attaaaactg gatgagatga tcgaggcaga accggaaccc gagatgattg aagggctgcc
     2461 cgcctctgat gcgctgacgc ctgccgatcg ctatctcgaa ctgttcgagc atgttcagtc
     2521 ggcgaaaatt ttccccgaca gtaaaacctt tcccgactgc gcacccaaaa tggacccgct
     2581 ggatatttta atccgctacc gtaaagtgcg ccgtcatcgt gattttgact tgcgcaagtt
     2641 tgttgaaaat cacttctggc tgccggaggt ctactccagc gagtatgtat cggacccgca
     2701 aaattccctg aaagagcata tcgaccagct gtggccggtg ctaacccgcg aaccacagga
     2761 tcacattccg tggtcttctc tgctggcgct accgcagtca tatattgtcc cgggcggccg
     2821 ttttagcgaa acctactatt gggattccta tttcaccatg ctgggactgg cggaaagtgg
     2881 tcgggaagat ttgctgaaat gcatggccga taacttcgcc tggatgatcg aaaactacgg
     2941 tcacatcccc aacggcaacc gcacctatta tttgagccgc tcgcaaccac cggtttttgc
     3001 gctgatggtg gagttgtttg aagaagatgg tgtacgcggt gcgcgccgct atctcgacca
     3061 ccttaaaatg gaatatgcct tctggatgga cggtgcagaa tcgttgatcc ctaatcaggc
     3121 ctatcgccat gttgtgcgga tgccggacgg atcgctgctc aaccgttact gggacgatcg
     3181 cgacacgccg cgtgacgaat cctggcttga ggacgttgaa accgcgaaac attctggtcg
     3241 cccgcccaac gaggtgtacc gcgatttacg cgcgggagcg gcctccggtt gggattactc
     3301 ttcccgttgg ctgcgtgata ctggtcgtct ggcgagcatt cgtaccaccc agttcatccc
     3361 catcgatctg aatgccttcc tgtttaaact ggagagcgcc atcgccaaca tctcggcgct
     3421 gaaaggcgag aaagagacag aagcgctgtt ccgccagaag gccagtgccc gtcgcgatgc
     3481 ggtaaaccgt tacctctggg atgatgaaaa cggcatctac cgcgattacg actggcgacg
     3541 cgaacaactg gcgctgtttt ccgctgccgc cattgtgccg ctctatgtcg gcatggcgaa
     3601 ccatgaacag gccgatcgtc tggcaaacgc cgtacgcagc cggttactga cacctggcgg
     3661 gattttggca agcgagtacg aaaccggtga acagtgggat aaacccaacg gctgggcacc
     3721 gttacaatgg atggcaattc agggatttaa aatgtatggc gatgaccttc tgggtgatga
     3781 aatcgcgcgc agctggctga aaacggtgaa tcagttctat ctggaacagc acaaaatgat
     3841 cgagaaatac catattgccg atggtgttcc ccgcgaaggc ggcggtggcg agtatccgtt
     3901 gcaggatggg tttggctgga ctaacggtgt ggtacgccgt ttaattggtt tgtacggcga
     3961 accataatat ttttacagcc agccgctaac ttcctgctgg ctgtaaaatt atcctcttca
     4021 ggagg
//
LOCUS       DAJTYG010000199         3969 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTYG010000199 DAJTYG010000000
VERSION     DAJTYG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.201"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..94)
                     /gene="yjbD"
                     /locus_tag="OO568_003970"
     CDS             complement(<1..94)
                     /gene="yjbD"
                     /locus_tag="OO568_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR6643074.1"
                     /translation="MRAPRITQKEMTEREQRELKTLLDRARIAHG"
     gene            complement(333..1682)
                     /gene="lysC"
                     /locus_tag="OO568_003971"
     CDS             complement(333..1682)
                     /gene="lysC"
                     /locus_tag="OO568_003971"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6643075.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2207..3856
                     /gene="pgi"
                     /locus_tag="OO568_003972"
     CDS             2207..3856
                     /gene="pgi"
                     /locus_tag="OO568_003972"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6643076.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1003 a         1048 c         1005 g          913 t
ORIGIN      
        1 gaccatgcgc aatccgcgcc cgatccagca gtgttttaag ttcacgctgc tcgcgttcag
       61 tcatctcttt ttgggtaata cgtggtgccc tcagtgagcc agagagttca gtgtacggga
      121 attcgtttgc gagcagaact gataatattc attttgcctt ctcttaacta tttatctctt
      181 tcgataatta attagagatg cagataaaaa aattaaaggc aattattctc cgataaagac
      241 gttggttaac agagtacagg ctcgtttatt gatgagcata gtgacaagaa aatcaatacg
      301 gcccgaaata tagcttccag gccatacagt atttactcaa acaaattact atgcagtttt
      361 tgcaccacct gctcggcatc ttcgccgggc accaggaagc acaggttatg gctggatgcg
      421 ccgtaacaaa tcatgcgaat gttgaacggt tccagtacgc cgaatacctc tttgccaacg
      481 ccgcaggctt ttgacaggtc attgccaatc aacgcgacca gcgccagacc ttcttccacc
      541 tccacccggc acagtgcgga aagctccatc agcagagatt gcgtcagcaa cgtatcgcca
      601 gtggaggttg aaccggtggt atcaagggtt aatgccacgc tcacttctga cgtggtgatt
      661 aagtctaccg aaatattatg ccgcgcgagg atgccgaaaa cttccgcgag gaagccgcga
      721 gaatgcagca tattcaggct gtgcaaagtg agcagagtct gattgcgacg aagcgccagc
      781 gcgcggaaca gcggcggatt ttcagtttta ttgcacacca gcgtaccacc tgcgcgtggg
      841 tctttgctgg agccgacaaa gaccgggata tcgctgcgta ctgcgggtag caacgttgcc
      901 ggatgcagta cttttgcacc aaaagttgcc atctctgccg cttctgcaaa cgcgatttca
      961 tcaatgcgtt ttgctgcgga aactacgcgt ggatcggtgg tgtagatgcc cgggacgtcg
     1021 gtccagatat caacacgaga tgcgtgtaaa gcctccgcca gcaaggctgc cgtataatcg
     1081 ctgcctccac ggccaagcgt cgttgtacga cctttatttt cgctaccgat aaatccctgg
     1141 gtgatcacta agccttcatt gaggcgtggg agcagctgca gcgcggccag ttccgccagc
     1201 gcggctacat ctggctctgc acgaccaaat cggtcgttgg tacgcatcac tttacgtaca
     1261 tcaaaccact gtgcctgaac atcgcgttcg cgcaggattt cgacaaacag cagggtcgac
     1321 atcagctcgc cgtggctgac cagctcatct gtcagcgccg gagacgttgc cagcgccgcc
     1381 gcttctgcca gaacagtaat gttctccagc agacgttcaa tctcttcacg gataacgttc
     1441 gggtaacgca gacgttccag aatggcaaac tggatgttgc gaatagcgtc gagtttttcg
     1501 aatcgctcgc caggttccag cccttccgct aaagcgacca gcagattagt gataccagca
     1561 gaagccgaga ggactactaa acgcacgttg gcatcagaaa gcacaatatc agcgctgcgg
     1621 ttcatggcgt caaaatcagc tacgctggta ccgccaaatt tggagacaac aatttcagac
     1681 ataactacct cgtgtcaggg gatccatttt cagccttggc acaagggaag agcggaagac
     1741 gggtgggcgc agagcgatac ttcgctacta ttttcaccca gaagtgctcc accacttgcg
     1801 aaacgcccga ctgcgaacgc ttctggtgac aacccagggg attcagcccc tgtagccgat
     1861 gatgaacgtg gccagccgtt caatcacctc ggcgatgcac cccctcaggt gttatcacag
     1921 gactggctcc tccaacaccg ttacttgggc aacgcgcctc ttctggcctg cgctagcgca
     1981 ggtagtacat ttataaataa agggtgagcg gggcggttgt caacgacggg gtcatgcgga
     2041 tttttcatcc actcctggcg gtcagtagtt cagctaataa atgcttcact gcgctaaggg
     2101 tttacactca acattacact aacggcacta aaaccatcac atttttctgt gactggcgct
     2161 acaatcttcc aaagtcacaa ttctcaaaat cagaagagta ttgctaatga aaaacatcaa
     2221 tccaacgcag accgctgcct ggcaggcact acagaaacac ttcgatgaaa tgaaagacgt
     2281 tacgatcgcc gatctttttg ctaaagacgg cgatcgtttt tctaagttct ccgcaacctt
     2341 cgacgatcag atgctggtgg attactccaa aaaccgcatc actgaagaga cgctggcgaa
     2401 attacaggat ctggcgaaag agtgcgatct ggcgggcgcg attaagtcga tgttctctgg
     2461 cgagaagatc aaccgcactg aaaaccgcgc cgtgctgcac gtagcgctgc gtaatcgtag
     2521 caataccccg attctggttg atggcaaaga tgttatgcca gaagtcaacg cggtgctgga
     2581 gaagatgaaa accttctcgg aagcgattat ctccggtgag tggaagggtt ataccggcaa
     2641 agcaatcact gacgtagtga acatcgggat cggcggttct gacctcggcc cgtacatggt
     2701 gaccgaagca ctgcgtccgt acaaaaacca cctgaacatg cactttgttt ctaacgtcga
     2761 tgggactcac atcgcggaag tgctgaaaaa agtaaacccg gaaaccacgc tgttcttggt
     2821 agcatctaaa accttcacca ctcaggaaac catgaccaac gcccatagcg cgcgtgactg
     2881 gttcctgaaa gcggcaggtg atgaaaaaca cgttgcgaaa cattttgcgg cgctctcaac
     2941 caatgccaaa gccgttggcg agtttggtat tgatactgcc aacatgttcg agttctggga
     3001 ctgggtcggc ggccgttact ctttgtggtc agcgattggc ctgtcgattg ttctctccat
     3061 cggctttgat aacttcgttg aactgctttc tggcgcacac gcaatggaca agcatttctc
     3121 caccacgcct gccgagaaaa acctgcctgt cctgctggcg ctgattggca tctggtacaa
     3181 caatttcttt ggtgcggaaa ctgaagcgat tctgccgtat gaccagtata tgcaccgttt
     3241 cgcggcgtac ttccagcagg gcaatatgga gtccaacggt aagtatgttg accgtaacgg
     3301 taacgttgtg gattaccaga ctggcccgat tatctggggt gaaccaggca ctaacggtca
     3361 gcacgcgttc taccagctga tccaccaggg aaccaaaatg gtaccgtgcg atttcatcgc
     3421 cccggctatc acccataacc cgctctctga tcaccaccag aaactgctgt ctaacttctt
     3481 cgcccagacc gaagcgctgg cgtttggtaa atcccgcgaa gtggttgagc aggaatatcg
     3541 cgatcagggt aaagatccgg caacgcttga ctacgtggtg ccgttcaaag tgttcgaagg
     3601 taaccgcccg accaactcca tcctgctgcg cgaaatcact ccgttcagcc tgggtgcgtt
     3661 gattgcgctg tatgagcaca aaatctttac tcagggcgtg atcctgaaca tcttcacctt
     3721 cgaccagtgg ggcgtggagc tgggtaaaca actggcaaac cgtattctgc cagagctgaa
     3781 agatgataaa gaaatcagca gccacgatag ctcgaccaat ggtctgatta accgctataa
     3841 agcgtggcgc ggttaatcat catgcgtgcc tgatgccgat atgtaggccg gataaggcgt
     3901 ttacgccgca tccggcaacc ggagcctgat gccaatatcg acattaatgc atagtatgag
     3961 gagttaaat
//
LOCUS       DAJTYG010000200         3876 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTYG010000200 DAJTYG010000000
VERSION     DAJTYG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.202"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(223..1581)
                     /gene="plaP"
                     /locus_tag="OO568_003973"
     CDS             complement(223..1581)
                     /gene="plaP"
                     /locus_tag="OO568_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6643077.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGSIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1571..1651)
                     /gene="yoeI"
                     /locus_tag="OO568_003974"
     CDS             complement(1571..1651)
                     /gene="yoeI"
                     /locus_tag="OO568_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6643078.1"
                     /translation="MRNPKKWGKFFAYATVITVKENNHVA"
     gene            complement(1849..2778)
                     /locus_tag="OO568_003975"
     CDS             complement(1849..2778)
                     /locus_tag="OO568_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6643079.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2824..3648)
                     /locus_tag="OO568_003976"
     CDS             complement(2824..3648)
                     /locus_tag="OO568_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6643080.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3731..>3876)
                     /gene="yoeB"
                     /locus_tag="OO568_003977"
     CDS             complement(3731..>3876)
                     /gene="yoeB"
                     /locus_tag="OO568_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR6643081.1"
                     /translation="PFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACR
                     YHY"
BASE COUNT         1059 a         1003 c          915 g          899 t
ORIGIN      
        1 agcagagcac cacaaattag cccgcttaat atcattgaaa acataaatta attaaccaga
       61 tgaatgttaa tggggaaatt tttcatgact ggtggaatgc agaccaataa ccaaattctc
      121 taaattagaa caaatggtta ttaatgagga tttcgataaa aaagaataaa aaaaccggag
      181 ccaaatgttc attcgactcc ggtttattat tagaaaagat gattatgcta cgtcttcgta
      241 ctgcggaacc ggattacgga agctcttggt gacgcaagcc aggtaaatca gaccgatagc
      301 agcccagatc agacccagaa ccattgagct ttcttccagg ttaacccaca gcgcaccaac
      361 ggtcagcgca ccacacatcg gcaggaacag atactggaag tgatctttca gcgtcttgtt
      421 acgcttctca cggatccaga actgcgagat gaccgacagg ttaacaaagg tgaacgccac
      481 cagcgcacca aagttaatca gcgccgttgc cattaccagg tcgaagttga ttgccagcag
      541 ggcaatcgag ccaaccagga tgatgttcat cgccggagta cgccatttcg ggtgtacata
      601 accgaagaag cttttcggga acacgccgtc acgacccatt acgtacatca gacgcgctac
      661 gcctgcgtgc gccgccatac cggacgccag tacggtaatg gtggagaaga tcagcgcgcc
      721 aacctggaac gctttacccg caacgtacag catgatttca ggctgtgacg catccggatc
      781 tttaaagcga gagatatccg ggaagtacag ctgcaggaag taagttgcaa agatgaagat
      841 catgccgcca atcagcgcgg tcaggaaaat cgcacgcggg atcacgcgct ctgcatcttt
      901 ggtttcttcc gacagattgc tgatgccgtc aaagccggta aaggagaagc acaggattgt
      961 cgccccggta atcatcggga taacatgcgc atcgccagac cagaacggac gcgtgctcgc
     1021 cagcgtacca gcgccttcac cttcaaatac gccataaaca accatgccca gaatcaccgc
     1081 gatcagcact acctgcaaca cgacgattac agtgttgaag ttcgctacgg atttcagact
     1141 acgcaggtta aaggcggtca taaaggccac cagcgccacc acaaacatcc acgatggaat
     1201 ggaaggcacc agagcttcaa aatagatttt cgccagcaga atgttgatca tcggcgcgaa
     1261 cagatagtcg agcagagaag accaacccac cataaagccg acagtcgggc taatggattt
     1321 ctgggcgtaa gtgtatgcag agccagcaga aggatagcgg cgaaccagct tcccgtagct
     1381 cagagccgta aacaggatcg caatcaatgc gaacgcatag gctgtcggca catgaccatc
     1441 cgtgaggcct gaaacgatac caaatgtatc aaacagcgtc atcggctgca tataggctag
     1501 acccatcatt acaaccggaa ctaacgtaag cgttttacgc aattccacgc gagaggtgtt
     1561 tggagtaacg ttatgcgaca tggttattct cctttacggt gataaccgtc gcgtaagcaa
     1621 aaaatttgcc ccattttttt ggattcctca gcgacaacaa ctgtcgattt ttagtaaata
     1681 tctatccggt acgaagcccg gcctcttggt atgaatgatt ggtttgaagc aaaaaataac
     1741 cgacgctaat taaacgtcgg tttttagtca ttttttgaca gcggcgcatt gtgcctaaat
     1801 gggggggaaa tgacaagaga atgagaggct tgtcataata atttttcttt aaatggctga
     1861 ttttccgtca tcagattttg cataaacacc ggcgatcgca ctatttgcta aaatttcatc
     1921 tcgccaccag ccgctggcaa gccctgcggt ctgttcattc cagccaatcc agacaggttc
     1981 aaagagagtt tgggcgacca ctttcttctc gattaacgcg ccactatcga gaaaacgttg
     2041 cgccagatac cgtggtaaat agccgcagcc aaggccgcta atttgcagct ccagtttggt
     2101 tttaaaatcg aaaacggtaa tggcttcttg ttcgtcgaga agttgtgagg ccgtagaagc
     2161 gcctgcctgc gcagtgtccc ccaccacaat cgcccgatat cgtttaatga tacgacggtt
     2221 taacggttct tcttcctggg ctaacggatg atgaggcgcg accgcaaaga cttgttcaag
     2281 atcgcccaac cgcgagaaac caaactcact ggaagatggt ggctcatgca tcgcgccaac
     2341 gataatatcc gctcgcccct gagtcagggc atcccaggaa ccaccgagta cgccattgat
     2401 aaacttcagg cgcgtcacgc tatgatgttg ataaaaagct tcaatgagag gcgcaagcag
     2461 agaaaaagga aacgtgtcgt ccacgccaat caccagctcg ttttcccagc cttcatgcag
     2521 tttgatcgcc tgcttttcca gttctcgcac ggtatgcaga acttcccgcc ctttctctaa
     2581 taacattttt ccggtgcggg tgaatttagc gcggtgtccg ctacgatcaa gcaattgaat
     2641 atttaggtcg ctttccagct tgtgaacggt gtaactcaga gcggacggcg tcttgtagag
     2701 tttggctgac gccgccgcaa aactgccctc tttttctatg gcatcgagga tcatcaacac
     2761 atccagcagt ggtttcatac acgccccctt gcagtgcatg cggcgtcctg ctggcgtgat
     2821 cgtttactcc agcggcatta ccagcggatc gggatactgg tattcaaatc ccagttcatt
     2881 acaaatccga ctgccatcaa taatcttacc tttgccgctg tccagactat ttctgaactg
     2941 cggaggttgc agccccagta aacgggccat ctgcggatag aaaacattac gcgcagggtg
     3001 agctggcgca catatattat agatgtgtcc gcctttaggt gcctgtaaca acagagtgat
     3061 agcgccaata acatcttcta aatggactaa attaacacca tgttcaccat caggggcggt
     3121 ttttccggca aagaagcgtc ccggatgacg tcccggtccg accaggcccg caagacgcag
     3181 aatatcgacc gaagtaccgg gtaaattgtg cagccagtct tcgagttctt ctaatactcg
     3241 tccactgttg gttaccggat tacgcggggt agtttcttta accgtgcctt gcgcgtcgcc
     3301 atagacagat gttgagctgg taaaaataat tcggggaata cgatgggcca gcgcgctatc
     3361 cactaactct tgtaccgctt gtaaatagaa ctcatcgccg gggccgctac gacgtgccgg
     3421 aagcgtaatg accagcgcat cggcatccat aagggcatcc agatcgtcag aatcgcaaac
     3481 taactcaggc tccatgcgaa gcagatagct atcaatgcca ctcattcggg ccgcttcgac
     3541 accatcttgt gtggttttac tcccggtgac ttgccagcct cgcgctgaaa gtgacatcgc
     3601 cagcggcata cctaaccacc ctaacccgac aattgcgacc tttttcatcc gttatctcct
     3661 gatacacctt agatctataa ggctacgcta gcgtatcaaa actgacaatt cattctatga
     3721 atgaatctgt tcaataatga taacgacatg ctgcaatgag cagtgaatca tcagtaaccg
     3781 cgtataccag acggtgctcc tctgtaatgc gtcgggacca gaaacctgac aaattatgtt
     3841 tcaggggttc tggtttcccc ttaccttcaa atggtg
//
LOCUS       DAJTYG010000201         3860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTYG010000201 DAJTYG010000000
VERSION     DAJTYG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.203"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(109..966)
                     /gene="sitD"
                     /locus_tag="OO568_003978"
     CDS             complement(109..966)
                     /gene="sitD"
                     /locus_tag="OO568_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6643082.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(963..1820)
                     /gene="sitC"
                     /locus_tag="OO568_003979"
     CDS             complement(963..1820)
                     /gene="sitC"
                     /locus_tag="OO568_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6643083.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     THKALEDRS"
     gene            complement(1817..2644)
                     /gene="sitB"
                     /locus_tag="OO568_003980"
     CDS             complement(1817..2644)
                     /gene="sitB"
                     /locus_tag="OO568_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6643084.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GNVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2644..3558)
                     /gene="sitA"
                     /locus_tag="OO568_003981"
     CDS             complement(2644..3558)
                     /gene="sitA"
                     /locus_tag="OO568_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6643085.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1053 a          960 c          925 g          922 t
ORIGIN      
        1 gattttacgt attttttgca tgatagaatt cgtacctcct cccaacgacc ttccatggct
       61 ttccggactt ctgtaggctg gcggacgatg ctgtcctcgg atgttgtgct aattgggtga
      121 taacagatcc tgtgtatggg cgtttccctt tgtgcgtgcg ttgatgctgg taacggtaaa
      181 cgtcatgatg aacacgaccg cgaataagac gacaatcgtc ggagctggtg cgctgtcgat
      241 aaaaaaggac agccagacac cagtcattga cactattacc gatatgccag tcgccagtag
      301 gagcgcaata tgaaagcgtt gtgtcaggag tactgcaatg gcgccaggcg caatgagcag
      361 agaaatagag agaatgatac ctactgcttt cagcgtcgcc acaatggtca gggaaaccat
      421 gcacagcagc ccataatgca gccatctcgt atgcaaaccg cttacctgcg cctgttgata
      481 atcaaagctg aacagcaaaa aatcccgcca ttttacgcta ataacaagtg taaccagccc
      541 ggcaataatc accgtctgga ttatatcgcc gatggttatc ccgagcatgt cgccgaaaag
      601 aatatggtca agatgtacgt ctggtttaat tgcaatatac aagatcagac ctgcggcaaa
      661 catgccagaa aaaacaatcc ccataactgt atcttgcttg attcggctgt tgtctttcag
      721 gtatcccgtc gccaccgcgc agaatactcc ggcgacgaat gcccctgtag ccagaggtaa
      781 ccccagaatc caggtaagca cgatgccagg aaaaaccgca tgactcatcg cgtcacccat
      841 cagtgcccaa cctttcagga ccagaaagac cgacagcagc gcacagggta tggaaactac
      901 cagtgaaatc agcagtgcgt gactcataaa ggtgaactgc agtggctcca gtaataatgc
      961 catcatgagc gatcctccag cgctttatgc gtacgccgac gatttgccaa aagtccatgg
     1021 gtgggcgcaa atacaaatgc taaaagaaac agcagtgtct gcgccaccac gatgattcca
     1081 cctgtcgcgc cgtccagaaa gtaactgacc cacgcgccga ggaaactggt tacactgcca
     1141 atagttacag caattatgag caggcgggga aaacgatcgg ttaacaacca tgctgttgca
     1201 ccgggagtca ccaccagaca gatcaccaga aaggcaccaa ccgtttgcag cgccgcaacg
     1261 gtcgaaaccg acagcagggt gaagaagagg atttttaatc tgcccggatg taatccaata
     1321 gcacgggcgt gattctcgtc gaaaaaggtc accataagat ctttccattt gaaaaaaagc
     1381 acgatgatcg agagaatgcc aatgatagtg agttgcagga tatccgccgg atcgattgcc
     1441 agaatattgc ccaggacgat ggtctgaatg ttcacggatg tcgggttcaa tgacaccata
     1501 aacagcccca ggccaaaaaa ggacgagaag atcaggccga tgatggcatc ttcttttaag
     1561 cgggtgcgtt ggttaagaaa cagcatgctg cccgctgcca gtccgccgga aaagaaggct
     1621 cccagagaaa acggaagccc cagcatatat gcccccgcaa cacccgggac aatagagtgc
     1681 gacagcgcat cgccaatcag cgaccagcct tttagcatca gatagcatga caggaaagcg
     1741 cagagaccgc cgaccattgc cgagacccac atcgcattga gcatatattc atagctgaag
     1801 ggttccagta gcacgttcat cttgcttccc tctgcaccgc tgacggtcga tgtgccacaa
     1861 aagggcgttc atcgtcagta ataatgcttt cttccgagcc gttaagtgtg acatggcgca
     1921 gtacgccgct aaaagccagc tccaggttct cggctgtaaa agtggtgtct gtcggcccgc
     1981 ttgccaggac tgtgcctttg accatgaccg tataatcgca aaatgtcgtg actgagccga
     2041 gattgtgcgt tgagacaagc atcgttttgc cttccgcgcg taactccctc aacaggctga
     2101 tgattttcgc ttcggttttc acatcaacgc cggtaaaagg ttcatccagc aggatcacat
     2161 taccctgctg tgcaatcgct ctcgccagaa agacgcgttt cttttgtcca ccggaaagct
     2221 cgccgatttg acgatggcga aaatccacca tatcgacacg ttccagcgca tcggtgacaa
     2281 tctgacgatc cctctttttg gctatacgca acatacccat atgcccatag cggcccatca
     2341 tcaccacatc ttccaccagt acaggaaatg accagtcaac ctcttctgac tgaggaacgt
     2401 aggcaaccag gtttttctgt agcgcctgtc gcgtgggaat acccagaaca gatatctttc
     2461 cgctggtcag acgcacaaat cccataatcg ctttaaacaa cgtcgatttt ccggaaccgt
     2521 ttaccccaac cagagcggca atagatccac ttggtactgt gaaggatgca tcgcgcagtg
     2581 ctgtgtgccc gttacgccag gtgacagtaa catcattaac gacaatgcct gcagattgca
     2641 tcattatttc tcccttttcc cggctttaat tccctgtacc agcgtactgg tagtaacctt
     2701 aagaaggtcg atatacgtag gtaccgggcc gttttctgtg ctcagggaat cgacatagag
     2761 cacaccgccg tagtgtgcgc cggtttcacg cgcaacctga cgcgctggtt tatcggaaat
     2821 cgtactctcg ctaaagactg ccgggatatg atttttctta actatatcaa caaccttacg
     2881 tacctgctgc ggtgttcctt gttgatcggc attaatcggc cacagataaa gctcttttag
     2941 ccccaaatcg cgtgcgagat aagaaaaagc cccttcactg gtgaccatcc atcgctgatt
     3001 ctcagggagt tccgtaatct gcttacgcag gggggcaagg gtttgggtaa tcttggcttt
     3061 ataagtatcg gcattacgtt ggtaggtttg tgcatttgcc ggatcgtatt ttatcaacgc
     3121 atcacgaata ttatcgacgt aaatcagagc attatctggc gacatccatg catggggatt
     3181 aggtttgccc tcatagggtc cttcggtgat ccctactggc gtcacacccg aagagacaat
     3241 tacttctgga accccattga gatgctggta aaagcgttgg aaccacaatt ccagattcat
     3301 accattggcg agaatcagtt gtgccccctg cgcacgttta atatcgccag gggtaggctg
     3361 atactcatga atttctgcac caggcttggt tatggatgag acttctgcag catctccagc
     3421 cacgtttttg gccatatccg cgatgatggt aaatgttgta atgaccttga atttttcagt
     3481 tgcacttgcc tgaaatgcga tcgagcaggt gagggcgagg caccccaaga gcatggttac
     3541 tttttttatt gagtgcataa tttagtacct atagtgagca aaattagaac aaacatagca
     3601 acggctatgt tttatagcat aacctataca aaatgaaaat aattatcatt aacagctttg
     3661 aggcctaaaa cagggattag gttgtttcct gctggcgaca tgatattgag gaaaaaagta
     3721 gagcagctat taggctttga aatgacttta tgaggaaaat tgttgatggc aaaaatgggg
     3781 caaaacgctg tataaggggc aaaaaagagt gggttatcgt agcttattgt tgtcgctgat
     3841 aatgtttaac atattgaaaa
//
LOCUS       DAJTYG010000202         3854 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTYG010000202 DAJTYG010000000
VERSION     DAJTYG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.204"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..536)
                     /locus_tag="OO568_003982"
     CDS             complement(<1..536)
                     /locus_tag="OO568_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643086.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRL"
     gene            complement(1198..2184)
                     /locus_tag="OO568_003983"
     CDS             complement(1198..2184)
                     /locus_tag="OO568_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643087.1"
                     /translation="MMTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSSTGFTRGTLQQDALNICDNVVRTINSKMV
                     DVLESSKLLKTVELPVLTGSLTAKADTIMDALYENTESSFGSEVTDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDIAYYNGADRGVFMMAKRFTALSFGCFRHDGENITV
                     VLSRDGNTQSHDLEILGKVFVVAEAATTVKMGTGSAAAVLPVVKRLSFTKTS"
     gene            complement(2266..2757)
                     /locus_tag="OO568_003984"
     CDS             complement(2266..2757)
                     /locus_tag="OO568_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643088.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSRESMDLLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(3052..3810)
                     /locus_tag="OO568_003985"
     CDS             complement(3052..3810)
                     /locus_tag="OO568_003985"
                     /inference="COORDINATES: protein motif:HMM:NF016283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR6643089.1"
                     /translation="MSTQPLMKHEVVLPVIVDGYHWACNQDRMWNLNELHKELELPSA
                     KEPGQWRSSVRTLLDQDANLHVAHGGSTPGTWATEEAAIAYAMWVSPDFYLKVIRAFI
                     ALRNDKVSEAKALAQDSKELKGLKPIARNWLEKLDNPKQGHTLTECLKNLDFRLGTKL
                     VSAKALNAVLKSGRIANPFYSRKLDGRGQPVFDISSGGWMTSFNPKGLENGYYRLQNN
                     HYNGQEGLKVLTKGYEWLKSNKVELVRLCAKGGL"
BASE COUNT          980 a          921 c          954 g          999 t
ORIGIN      
        1 agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg cgtagacata
       61 taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg gtctgatact
      121 aatgccatat agccccataa actatccctc agccagacag catcatagtg ggtttctgcc
      181 gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg tttctccgtc
      241 aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgaatttc gtctttggta
      301 tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg ataattagga
      361 tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct ggaattaaga
      421 atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt gccgcacgaa
      481 aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata atccatcaat
      541 cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg tattttacct
      601 gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa aatggggaag
      661 tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag aaaatcagat
      721 acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa gggagccgag
      781 gacaactagc taacttcttg tttcttaaag actgatgaat ccagtggtta cagtgatggt
      841 tacacatgga gaggctaaag gtcaagactt gaagtacgta acattcccac aaaaggaata
      901 gaacacagtc acggactacc cggccctttg gtctgactga gggtcgggct acggggtaaa
      961 tcgtcaggct tattgtatta gatggtctct cacatttttc agtcaaaaac tcatcgtact
     1021 ttaggccaga ccatcagcat gactatcaga gccagcttta gcaggaagac cacgagatag
     1081 actttgtgta ggtccattag gtcctcctct ttccgcaccc acgaactaga ttactagtac
     1141 aaaataaagg ccagacatcc agttacggaa gtctgaccaa taagtcgtta ttgttagtta
     1201 gcttgtctta gtaaagctca ggcgtttgac tacaggcagg acagcagcag cgctgccagt
     1261 ccccatctta acagtcgttg cagcctcagc gaccacgaag accttaccga gaatctcaag
     1321 gtcgtgagac tgggtgttgc catctcggga taagaccaca gtaatattct cgccatcgtg
     1381 acggaagcaa ccgaagctca gagccgtaaa gcgcttagcc atcatgaaga cgccacggtc
     1441 tgcaccgttg tagtaggcga tgtctgtacc cagcatatcg acaatagcgt cttcgccacc
     1501 gaagccctgc ttagcagcca gacgggaaag agccttgagg tgtgactcat gggcgatgat
     1561 accgtaatca gtaacctcag aaccgaaaga agactcggtg ttctcataca gagcgtccat
     1621 gatagtgtct gccttagcgg tcaggctacc agtcaggacc ggaagctcta cggtcttcag
     1681 cagcttagag gactccagaa cgtcaaccat cttagagtta atggtacgga ccacgttatc
     1741 gcagatgttc agagcatcct gctggagcgt cccccgagtg aaaccagtag aggacttctg
     1801 gtcccagagc tttgtctgat gagacacgag gatagcctgt tcaatcggct caacggtgat
     1861 ttcagtacca gacggagcgg tcacgacttc aggtcgctta ccaccacgga caggaacgac
     1921 cacagagcgg tcagccagag tgtgagcaat gattggcgtc ggtaagctgt tacgggtcgg
     1981 caggacttct acagggaacg gaatgttttc ctgagcgtct ttaagtacac cgttcagcat
     2041 agccttaacc gtcttagcta actggagggc atagacctgt gggtcagcgt tagtaggcag
     2101 accttcattg acctgaatgt tgtcggtgtc ggtgatacgt actggctcaa taccgaactg
     2161 gaaagcgtct ttaatcgtca taataatcaa tctcctaaat tagtttaaat actgtgagtc
     2221 ctcaaaactg aggagtccct agcatcgggt cagggactgt gtgttttaca ggctgtccat
     2281 ccagttaccg cctgactcaa ggtcagcgac gatagcggca cgggagcggc cagcggaatg
     2341 ggtagcactg cggaggtttt caccataggt ccggcgttca gctttgacct gttcgcggcg
     2401 ctgtagctct ttttgccatt cgtcttcggt catgaacatt tcgcgggtac gctctttctg
     2461 agacgctagc tctttagcga tacgctcagc ttcagctcgt ttgttcatct ggcggcgttg
     2521 gtaggcggtc atcaggtcat tactgaagaa gttaccaatg gtcacgccag cttcagccag
     2581 agcttccagt aaatccatag actcacggct ggtagacggc aggccggaca ggtagcccgg
     2641 aatgagggtc ttgatagcga gcgacaacgg ataggactga ccgtccagac tcacagtgtt
     2701 ttcataaagg ttgatgtcgg tgactgacag cttccagttg ttgttgttga acatcatagg
     2761 taattctcct cttgagagtt gagactatcg gtatcgcagg cacagtaagg cctactggtc
     2821 tccccgcatc ggtcatctca aaaggtgttt aaattaggta ttgagtaggt gagctactaa
     2881 cggttaagcg gcagtttcat agcgaccagt tgggctatta gctcgtctcg ggattggtgc
     2941 tggcgggtct catcttccgg gtctagacca agctctctga ggaggacttc agtccggtag
     3001 ccacggtaac ggagagtcat gcggccctca ggtgtgcgcg ggtcaatcat cttagagtcc
     3061 tcccttagcg cacagacgaa ccagctcgac cttgttggac ttaagccact cgtaaccctt
     3121 ggtgaggacc ttaagcccct cctgaccgtt gtagtggtta ttctgaagac ggtaatagcc
     3181 gttctccaga cccttcggat tgaagctggt catccagcca cctgacgaga tgtcaaatac
     3241 aggctgtcct cgaccgtcca gcttgcgact atagaaggga ttagctatac gccctgactt
     3301 caatacggca ttaagagcct tggctgatac cagctttgta cctaacctga agtcgagatt
     3361 cttaagacac tctgtcaggg tgtggccctg cttcgggtta tccagctttt ccagccagtt
     3421 acgggctata ggcttgagac ccttaagttc cttggagtcc tgcgccagcg ccttagcttc
     3481 cgacactttg tcgttacgta aagcgatgaa agcacggatg accttaaggt agaaatcagg
     3541 tgagacccac atcgcatagg caatagccgc ctcttcagta gcccatgtcc ccggggtcga
     3601 accaccatga gcaacatgca aatttgcatc ttggtcaaga agcgtcctga cgctagaacg
     3661 ccactgaccc ggctccttag cagatggaag ctcaagctct ttatgtagct cgttcaggtt
     3721 ccacatacgg tcctgattac aggcccagtg atagccgtca acgatgacag gaagaactac
     3781 ttcatgcttc attaagggtt gagttgacat atcgttgtac tccttttgtt atgcctcctg
     3841 aagtcacacc ctga
//
LOCUS       DAJTYG010000203         3670 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTYG010000203 DAJTYG010000000
VERSION     DAJTYG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.205"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            107..418
                     /locus_tag="OO568_003986"
     CDS             107..418
                     /locus_tag="OO568_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HCR6643090.1"
                     /translation="MDAHWKTVLKMSVKRWLWLIIVSVLMFATTGSLLWCQGMKINAN
                     MNILREQKESLEKLNAKTWGVRYNEDSNGRFLVLPEGMKAETNWTMENGKQNAVRLVQ
                     E"
     gene            425..658
                     /locus_tag="OO568_003987"
     CDS             425..658
                     /locus_tag="OO568_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6643091.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(1237..2355)
                     /locus_tag="OO568_003988"
     CDS             complement(1237..2355)
                     /locus_tag="OO568_003988"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6643092.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFIL
                     LMVFFLTFYLSKKILIPTSKIESILIKKMNIINAKKAN"
     gene            complement(2473..2562)
                     /locus_tag="OO568_003989"
     CDS             complement(2473..2562)
                     /locus_tag="OO568_003989"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643093.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(2998..3159)
                     /locus_tag="OO568_003990"
     CDS             complement(2998..3159)
                     /locus_tag="OO568_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6643094.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
BASE COUNT         1075 a          722 c          859 g         1014 t
ORIGIN      
        1 cagggaactg acctcaaacg aatctttaat caggaacgct atcaacgacc atacatcagc
       61 cctgaaagaa cttctggaga ggtatcagaa aacgaccgtc gatacgatgg acgcacactg
      121 gaaaaccgta ctgaaaatga gcgtgaaacg ctggctgtgg ctgattatcg tctcagtact
      181 gatgtttgcc acgaccggga gcctgctgtg gtgccagggc atgaagataa acgcgaacat
      241 gaatattctc cgcgagcaga aagagagtct ggagaagctg aacgcgaaga catggggcgt
      301 gcgttataac gaggacagca acggacgttt tctggtgttg cccgagggga tgaaagccga
      361 aacgaactgg acgatggaaa acgggaaaca gaatgccgta aggctggtac aggagtaaca
      421 gagcatgacg gagctggaaa gacatttgct gaacgcatta gagcaactgc aacaggacta
      481 tatgcagcgg ctgaacgaat gggagagcgc cttcgtggaa ttgcagaaaa tgttttcgct
      541 tacgcaacgg gacaacgcga tgctgaacga gcgggtcatg cagttgagtc agcaggtgca
      601 gcacttgagc gagcagacag aacgcttgag ccagttatac agcgagaaca ggagataagg
      661 gacgaacggc tgatacagga atgcgaacat gcgttatccc tggagcgtga gcgacagccg
      721 gaaatacagg aacggacact ggatggccct tcgctgggat ggtaatcaga gacaggctaa
      781 aatgttgtca gcgcgtttcc ggtgcatttt ttgggataat ggttggtttg ggggcaccgg
      841 gaacgcgctt cagtcagacc ttgctgaaaa cataggaacg cgttaaaacg catacgggcg
      901 cgattctggt gtgtcttttg ttgctgtgtt ttgcctctat tgccagatcg cgctcattcc
      961 cctgaacttc tgttttcccg ctcaggtccc cgtcttcttc gcttccgaaa gcatgaaaaa
     1021 aatccgtttc agctgtctct ggctggcaat tcctgcgtga ttcacatggc tgcatagcta
     1081 tgcatgtggt taaaatttac cgggtgcgat cgcggcagtt tttcgggtgg tttgttgcca
     1141 tttttacctg tctgctgccg tgatcgcgcc gaacgcgttt cagcggtgcg tacaatgaga
     1201 tgttatggta aattcaagtc ttagatttta tgatgtttaa ttagcttttt ttgcgtttat
     1261 aatgttcatc ttttttatta aaattgattc aatctttgat gtgggtatta atattttctt
     1321 tgacaaatag aaagttaaaa aaaacaccat taatagtata aataaatacc caaaaacaga
     1381 agcgttatta atttcaatta ttgatgttaa tctgtatatt actacatgct gaatcaacat
     1441 ggatatgtag atgcatttag catacgagtt gaaaaaatag aggttaaatt tcttattaat
     1501 tattaatgct gctttgtaaa tgataatgct tatgcacatt aaaggtatta ttgataactg
     1561 gtgattgact atgtttgttt tgaactcatt aggccctatt aataatactg tcaatataca
     1621 tataacaacc gatagaataa ctgtggtgtt tttttgtaat ttatttttgt atttaaatgc
     1681 aagcatccca accataaact caggaagtcg gcaagcaaaa aaccaaaatg ggaagtttaa
     1741 aaatgatgat gagatatttg atatcgggaa tgttgtaatt gttatcacaa ataaaaaaaa
     1801 tatgttacgt ttaaatgtaa aggtattcat aacgtgaaaa actatcggtg atatcacgta
     1861 cagcataata acgcacccaa taaaccactc gcctgtaagg aaaagaaaat cataagagaa
     1921 atgggcgttt aatataccat caagggcaaa aaatactgga attattctag tgtactggcc
     1981 tcctgttgtc attattgata cgagagggac tgtattaatt atctctttat ttaaataaga
     2041 caataaaatt aagagtaaag agataaaaaa gtaagtgata atataaggag taaggatcct
     2101 tactcctttg tcaaggaaat aatcaatggt gttctttcta tctagtgaaa taaatgccaa
     2161 atatccagat ataaaaaaaa acaagctcac acctacctgg ccaagaaccc ctccataaaa
     2221 tgatggtatg aaagatagcc tggtttggct aaatatgtag ccataatgag atataataac
     2281 aaaaatgcag gctgttactc gcaatgtatc aattgacgga tctctattag acataacctt
     2341 tatttcgctg ttcaaattat cgcctttagt tgatgatgta aaattaacgc tataaataag
     2401 ctacacattc tagagcagcg aaaggatgat tcaagtattt ttaataataa aacgcattga
     2461 taatgtttat gttcagatgg tgctgatagc tttgtcatgc tgttccactg atgttgataa
     2521 cgacagagac ttttgtcctt ttcttgttag atgctgcctc agattcggat tatggcgtta
     2581 aaggggtttg acgctcaacc gaacggtttc acacgttaag agctttttgt ctgataaatc
     2641 aaaatataat ggacattgaa aaacgccctc aaaacggtgt ctttttaatc gaagattggc
     2701 acaaaacgga caccaccgtc cattagtcga ttttttagac ggcttttctc gcctaacgtg
     2761 agttttcgtt cggttgagca tcagacccct tttatattgt agatttctat ttttagacag
     2821 ttggaacagg acaattaaca acagatctac ttgagatcat ttgcttgtcg tcgtagtctc
     2881 tttcccagaa acgaaaaaac cgccctgcaa ggcggtttct cgaaggttct cagagcgacc
     2941 aactctttga accaaggtaa ctggcttatg aggagcgcag taaccaaatc tgtcctttca
     3001 gtttagcctt aacaggcgca taacttcaag actaactcct ctaaatccgt taccaatggc
     3061 taccgccact ggcgatttgt cgtgtctttc cgggttggac tcaagttgat agttaccgga
     3121 acgggcgtgg tagtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     3181 tgcctacccg gaactgagtg tcaggcgtgg aatgagataa acgcggccat aacagcggaa
     3241 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcca ccagggggaa
     3301 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcggtttt
     3361 tgtgatgctc gtcagggggg gcggagccta tggaaaaacg ccatcggcgc ggccttcttc
     3421 ctgttcctgg tcttttgctc acatgttctt tccggcgtta tcccctgatt ctgtggataa
     3481 ccgcatttcg tttttgagtg agtagatacc gctcgccgca gtcgaacgac cgagcgtagc
     3541 gagtcagtga gcgaggaagc ggaaaagcgc ctggacgtgc attttctcct tacgcatctg
     3601 tgccgcattt cacacccggt atggcgcact tttcatacaa tctgcactga tgccgcatgg
     3661 ttaagcccgt
//
LOCUS       DAJTYG010000204         3545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTYG010000204 DAJTYG010000000
VERSION     DAJTYG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.206"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..222
                     /locus_tag="OO568_003991"
     CDS             <1..222
                     /locus_tag="OO568_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643095.1"
                     /translation="IANFIALQINGSKTGFAASITGFTQTISAGTISFLSVTVSDNGV
                     GPGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            939..1127
                     /locus_tag="OO568_003992"
     CDS             939..1127
                     /locus_tag="OO568_003992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643096.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            1224..1403
                     /locus_tag="OO568_003993"
     CDS             1224..1403
                     /locus_tag="OO568_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643097.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            1455..1649
                     /locus_tag="OO568_003994"
     CDS             1455..1649
                     /locus_tag="OO568_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643098.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(2448..2784)
                     /locus_tag="OO568_003995"
                     /pseudo
     CDS             complement(2448..2784)
                     /locus_tag="OO568_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1133 a          761 c          693 g          958 t
ORIGIN      
        1 atagccaatt ttatcgcatt acaaataaat ggttcaaaaa caggatttgc cgcatctatt
       61 acaggtttta ctcaaacaat aagcgctggt acaatcagtt tcctgtcagt aaccgtaagc
      121 gacaacggtg ttggtcccgg gactacttta ggtcttggtt cactaataac ttcattactg
      181 tcaatagccg taatcgccat tgcaggaaat aataaaaagt aagtgtactc tgactgcgta
      241 tgtatctact ttgtgtaatg caggtgtcta ttgaaaataa ttcttttcgt gggcatctgt
      301 tgtataaaat aaaaaattat cgtaagtact ctatctgcga tgtcttgtga aaattatcct
      361 gtctggcaac aatcgcgtcc atctgtatta atggacgcga acgatgaact ctcaaacaac
      421 actagatatc accggctttc gcacttattt gccagaatca ttacgtctca gattcgacga
      481 gcaattatct gtccgggcta tcgcacagca gttaagactc agttattccc ccattcacaa
      541 tctctttcaa cgaattaacg cctcaggtat tgtctgacca tgacccgagt ccctttcatt
      601 cgcccaaccg ataccatact ttatgccaac agaaagaatg aatgaaccgc gcctaaaatc
      661 agcaaggaca catggcacaa agtgcgacat gtctcacatt taaccccagt gggggcaaca
      721 agtataaaac cgttttttga ttaagtggtc gaatatgaaa aggggaaggt ttatgtgaaa
      781 aatacctgtg ttttctttga aaacacacaa cagtatcagg ttatttatgt ttatacagtg
      841 cgttccaaac tgacctgaat aattacgcaa gttgcaacaa taaaacattt ttgccgtagt
      901 gtgaggtact ccggtaaatt gctttccagg tgcagacaat gactaacgaa gaactacagg
      961 caatcgcatt atccatgctt gagcgccagc gcgaaaaagt acaccagcaa cgagagtctt
     1021 tgcttaaccc atctccattc accaggccaa aacgattgga gcttgaagca ttcttggaca
     1081 aatacccacg gcggcttaaa tcaggcaaaa ccagaccacc aggctgatag ctcacagttt
     1141 gttctccgat tcaataaaac ggataagaac catcagcgcg gtagcgtaag gcactacagc
     1201 gcattgcttt acgagtaata acaatggatt tatcaccaga agacgcaaag tttttacgag
     1261 aactgcaaca ccgtatgctc agagaagcca ttatcgaaca gcaaacgggc atgagcgcca
     1321 tagacccatt gcgatacaaa cgggcctcat ctcctttagt tgacgacaat tccaatgcgg
     1381 ataaaacacg tccaccacac tgattacatt tttcattagc acagtggtgt gatgcagcat
     1441 acgaggaaaa ccggatgaat gagaacaaaa taaaaaggct ggagcaactg ttacaggcac
     1501 gccaacggga gtttgccact aaatggggac gtgccgcgct gccacatgaa cgcctaacct
     1561 attttccact tgaagacctc attaaaaata atggcaaaaa aatcaggtct gataagaaac
     1621 acttcttaga atgcaatgaa gacaaataac aacaatctgc atattgccac caaccacaga
     1681 tgtggcatcg ttatcagcgc acttgatttt ctttatatcc actgacgagc ttgccggggc
     1741 acctttgttt gccggtcatt ctggtatcat gcagatattt acggcgtaca agcccactag
     1801 catgacattg taataaggcc tgagcctaac gctttaatgt gtgtcataca ctcagtggcg
     1861 taatcgttga caatatggtg catgtaatcg taagcgtcca gcgaaatcca tctggtcata
     1921 atctgaagca tccgacaaag tggtgtccac caaataagtg atctgcgccc aatatcctga
     1981 gccggaactt tgacaaaggc caacgaaaaa tgggagcgga tgttacagag ttctcggtac
     2041 aggggaaaag tggtacctgt cgccagttct ccgatctgtt taaccgggaa attatctcct
     2101 acagcttgtc agaaaggtcg gtgatggagc atcgttaata cgatgctata cgatgcattc
     2161 acagtgcccg gccagaggat gccccgtcgc tgcatatgga tcagggttgg caatatcgaa
     2221 tcgcaggcta taggcaaagt taaagcccca tggagtagca caaaatatgc cgcacaaagg
     2281 aaacggtctg aataacgcag tgatgaagaa cttcttcagc acactgctaa aacgcatagt
     2341 gatcgagcgc tgattctggc gaacaactga actaatacat cagaatctgc attatgttaa
     2401 ataaatatga aaagatggtt taaatacccc gttacttttg acttactcta tacggtatcg
     2461 catcgtttaa tattcgcacc ggccagattt ttatttctat tagttgtcac aatactgaat
     2521 gcgtacgacc acagtattct ggctcctgtg tggttatgct ttaattctgc gttccgggca
     2581 gataagcagt tgcttgcagg aatccttctt gtgttaatgt cagttcccct ttttaccagt
     2641 gctgatttcc acattccgtc caacagagct tatagccttt ccctggatta tagcattgtc
     2701 cggctgaagt tctttttgaa taataataga agcactgctg gcagatccag tccgtttttc
     2761 ataacccact gtactgataa ccataatcta atcagtagaa attgagtcga aaataagcac
     2821 tactccatac aggataatta gaggtcagtt tgattattca caattcatca tcagcatttt
     2881 ctatttctga cgaaatcaac atgaaaataa ccatatatga taattattat aataacggct
     2941 ttaattggaa tacatatatt acaacgtatt atatataatt ggtattctgg gaactatatt
     3001 ctcaaaatac agtagaaacg aggtatgttt ctggtggaaa ggacagtggg attaaaaagt
     3061 aagaattgat aaaaaaaacg ccagcaacac atagtgccgg cggagggaat accccatgga
     3121 gaaaatgtga tgcctagagc atcatgaata tataattaaa aatagttagc gttgtcaata
     3181 ctttcacaaa aataattttc gtagtataga aagatatttt tatgcatgac ctacctgaat
     3241 ttgctccggg tagaggttat aaataaaaat tgaatcacga caaacacaat attcatagta
     3301 tggcgatgcc tacgccagca agaatagcgc caataatatt gggaatataa tagacaccag
     3361 acgcacaggc atctcactcc ttaacaaaca ataatcagga tatttacttt tacccagcta
     3421 actgtttaca cccaaagtgc acacaattaa ccattcaata acaaatggca atatccatag
     3481 ccatacgact ttaccttgta atgttcggta tttctttata attattctgg gaaatctatc
     3541 attta
//
LOCUS       DAJTYG010000205         3522 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTYG010000205 DAJTYG010000000
VERSION     DAJTYG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.207"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(66..1508)
                     /gene="cusS"
                     /locus_tag="OO568_003996"
     CDS             complement(66..1508)
                     /gene="cusS"
                     /locus_tag="OO568_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6643099.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            complement(1498..2181)
                     /gene="cusR"
                     /locus_tag="OO568_003997"
     CDS             complement(1498..2181)
                     /gene="cusR"
                     /locus_tag="OO568_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6643100.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2338..>3522
                     /gene="cusC"
                     /locus_tag="OO568_003998"
     CDS             2338..>3522
                     /gene="cusC"
                     /locus_tag="OO568_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6643101.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEKAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQ"
BASE COUNT          866 a          911 c          922 g          823 t
ORIGIN      
        1 ataggtatga ctattgcggc acgttatttt tacactggtt ataaaagttg ccgtttgctg
       61 aataattaag cgggtaatgt gataacaaac cttgtccccc gcgcatctga cgttaccgca
      121 accgtgcctt tatgcgcgac aacaatcgat ttcactatcg ccagcccaat accgctacct
      181 tcaccttttc gctggcggga ggggtcaacg cgatagaaac ggtcaaacaa tcgcggtaag
      241 tgctcgggcg caatgggcgt accggggttt tcgacggaaa cttgcacctg gtgatcgacc
      301 gtctgacagc gcactacaat tgtctctctg gttggcgtat aacgcagggc attagaaagc
      361 aggttgctta gcgcccgacg cagcatcaac ggatcgcccg caacctgacg tttgtcgcca
      421 acaaaccgca actccacgcc gcgatcttcc gctagcgcct cgaaaaaatc gaacactttg
      481 ccgacttcat ccgccaggtt gagcattttc ttttcgggga ttagctggtt gttatcggcc
      541 tgagcgagaa acagcatatc gctgaccatt ttcgccattc gcgtcagctc ttcgagatta
      601 gagtagagca catcttccag ctccttctgg ctgcgcgact ggctgagggc gatttccgtt
      661 tgcgtaatga gattagtaat tggtgtgcga atttcgtggg cgatatccgc tgagaaattg
      721 gactggcgag taaagacatc ctcaatacgc tcgatcatat ggttgaacga cagtaccagc
      781 tgttccagct caatcggcac ggtctgcggg tcaaggcgaa cgtcgagatc tttcgaggtg
      841 atattctgga tttgacggct gacgctgcgg atcggcgcgt gacctttatg taccgccaac
      901 agcacgataa agacgatcag gatgctgatt accgatgcgg tcataataag tttattcatc
      961 aaatcattta tgtaatgaag atgaaaatcg atcgacagcg cgatgtagag cgtataaatc
     1021 ggtttgccgt ccaccaacgg gccaaccggc aagttaatca tccgccagtt gctgtgttcc
     1081 atatgcccgt gaccgtggcc tggcatcata atcgtcgggc cggaaaggag atacacctcg
     1141 ccaccccgcg cgtctttatc gggtatggca tcacgtgcaa actcgcggat atcaggcgca
     1201 ccgggggagt gatacaccgt tttgccgtgg ctatctgcca gggaaatcaa cacgttggaa
     1261 taaccactga cgatatcttc cagcgtcatt aagcgtcggg cttgcgtttc gtcagggtga
     1321 tttagtaccc gttcaagggt ggcgctaatc tcttttaaat cattaatgtc ctgctcggca
     1381 aaatgcactt ttactgagtg gatcatgatc catgcaaagg cgaaaaacgc cgcgatggtg
     1441 gccaggctga taaaaaaggt caggcgggtt gccagcgaaa acgggcgctg aaatggctta
     1501 ctgaccatcc ggcacctcaa gcatgtaacc cacgccgcgc acggtttgaa tcagcttcgg
     1561 ctcaaagtcg ttgtcgattt tgccgcgtag ccgcttcacc gccacatcaa tggcgttagt
     1621 gtcgctgtcg aaattcatgt cccatacctg cgaggcgata agcgagcggg gcagcacttc
     1681 gccctgatgg cggagaaaaa actccagcag agtaaattct ttactggtca gagtgatgcg
     1741 cgtgccgctg cgggtgactt tgcggctgac gagatcgacc atcagatcgg caacctgaaa
     1801 ctgactttcg ataatcaccg ccgccccgcg ccgcaggaga gtgcgcaccc gcgccagcag
     1861 ttcagcaaaa gcgaacggtt tcaccaggta gtcatctgcc cccaactcca gccccttgac
     1921 gcgatgttca atggtgccaa gcgcggtaag caacagaatc ggcatccctt tattggcgga
     1981 gcgcaacatg cgcacgatat cccagccgtt cacgtctggc agcataatat cgaggattat
     2041 caggtcataa tcaccggtca tcgccagatg gtagccattc agcccgttgt cggccaaatc
     2101 gaccacaaaa ccggcttcgg ttaacccttt ggtcaagtat tctccggttt tcttttcatc
     2161 ttcgacaatc aacagtttca tatttcctcc gcatgttgcc cgggcaattc tagagtagcg
     2221 ggatcagatg gcaatcgctt attggcaaaa tgacaatttt gtcattttcc tgtcaccgga
     2281 aaatcagagc ctggcgagta aagttggcga cataaaatca ccagaaatta tgagcctatg
     2341 tctccttgta aacttctgcc attttgtgtg gcccttgcgc taactggttg ttcactggca
     2401 ccggattatc agcgtccggt aatgcccgta ccgcagcagt tctcactcag ccagaacggc
     2461 ctggttaacg cagcggataa ctaccagaac gcgggctggc gcaccttttt tgttgataat
     2521 caggtgaaga cgctgattag cgaggcgctg gtgaataacc gggatttgcg catggcgacg
     2581 ctgaaagtgc aggaggcacg ggcgcaatat cgtctgaccg atgccgaccg ctacccacag
     2641 ctcaatggcg agggcagcgg cagctggagc ggcaatctta aaggcgattc agccacgacg
     2701 cgggagttct cgactggcct taacgccagc tttgatctcg attttttcgg tcgcttaaag
     2761 aacatgagcg aagccgagcg ccaaaattat ttagccactg aggaagccca gcgcgcggtg
     2821 catattttgc tggtttctaa tgtcgcgcaa agctatttca atcagcagct ggcgtatgcg
     2881 caattgcaaa tagccgaaga aacgctgcgt aattatcagc agtcatatgc gtttgtcgaa
     2941 aaacaactgt tgaccggtag cagcaatgtt ctggcgctgg aaaaggctcg cggggtgata
     3001 gaaagtaccc gcagtgacat cgctaaacgt cagggggaac tggcgcaggc gaataatgca
     3061 ttgcaactgt tattgggaag ctacggcaag ctaccgcaag cgcagacagt aaacagcggc
     3121 agcctgcaaa gcgttaaatt accggcgggc ttgtcgtcgc aaatcttatt gcagcgcccg
     3181 gatattatgg aagctgaaca cgcgttaatg gcggctaatg ctaatattgg tgctgcgcgt
     3241 gcggcatttt tcccgtctat cagcctgacc agcggaatat caaccgccag cagcgatctg
     3301 tcgtcattat ttaacgctag cagcgggatg tggaatttta ttcctaaaat tgagatcccc
     3361 atttttaatg ccggacgcaa ccaggccaat ctggatatcg ccgaaattcg ccagcagcag
     3421 tcggtggtga attatgaaca gaaaatccag aacgccttta aagaggtggc agatgcgctg
     3481 gcattacgtc aaagcctgga cgatcaaatc agcgcccagc ag
//
LOCUS       DAJTYG010000206         3225 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTYG010000206 DAJTYG010000000
VERSION     DAJTYG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.208"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..258)
                     /locus_tag="OO568_003999"
     CDS             complement(<1..258)
                     /locus_tag="OO568_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR6643102.1"
                     /translation="MNPSQHAEQFQSQLENYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVL"
     gene            complement(255..923)
                     /locus_tag="OO568_004000"
     CDS             complement(255..923)
                     /locus_tag="OO568_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6643103.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(987..1625)
                     /locus_tag="OO568_004001"
     CDS             complement(987..1625)
                     /locus_tag="OO568_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR6643104.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     ATAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(1638..>3225)
                     /locus_tag="OO568_004002"
     CDS             complement(1638..>3225)
                     /locus_tag="OO568_004002"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCR6643105.1"
                     /translation="LSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEM
                     KRLNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEI
                     PPGNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTG
                     GNTCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYC
                     YSKGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLAYGSYAIAER
                     WNLGKEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGL
                     PLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPM
                     TVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKPMQTKDM
                     RNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETL
                     QRLGLEDIAADLAAHNLLPA"
BASE COUNT          803 a          877 c          731 g          814 t
ORIGIN      
        1 caatacaaag agtaacgcat tcgaccagtg ccataatctg accgctttgc tgtaaagata
       61 aaccttctca ccatgatcgg tggctgattt cttcacgcca cgagcacgaa gcaaggcgtg
      121 cagccccagc accaaccaca tcccaaccag caatactccg gcaatgatca accacaccgg
      181 ccaaaattcg ggagtgaact gtggcacata gttctctaac tggctctgaa actgttcagc
      241 atgttgcgac gggttcatac attttccttt ttaatcaaat gttgaccgaa ccggcgatac
      301 agatgcggtt ttccggcacc aggtagctga tattgatagt atttattatc ctgcaaccac
      361 gcctgaattt ctgggctatc ttcgcgacca aagatcaatg cgtgttccgg acaggcgctg
      421 acgcaaatgg gcggaaagcc ttttgccagt cgggactcag cgcagaaatc acatttatct
      481 gccactttag tgacagggtt aaggtagcga acctggtacg gacaagcgcc aatgcaataa
      541 ctgcagccga tgcactgcga tttttctacc cgcacgatcc cctgctcatc acgccacgat
      601 gcccccgtcg ggcatacgtc gatgcacgga gcatcttcgc agtgctgaca tgactggcga
      661 aaaaagtgat actgcgtttc gttgtcgtta tcggtgactg gaatgtgtgc tatcgataaa
      721 cggcttccct gagcggggac gtggttagtt ttacggcatg cgcgggcgca aatattacag
      781 ccattgcacc gtgactcgtc atgaatcatg gcataccgaa cttccttttc ttgcctcgtg
      841 ttcgctaaca gagaagacgc agaaccagta aaaaagatta ctgttcccat ccccagaaca
      901 aatttgcgtc gagtgaagga catctgatta tcccctaata cagtggctgc aactgtccac
      961 cctatccatg acatctccct gaaatttcag gggttctgaa actgctctac ctcgaaccat
     1021 tcgcataagc cataagcctt gccataggtc ataaatcgct cgctaaacaa gtacaacacc
     1081 gccgtcgatg actccacctg ccgtatatcg gcatattcag gtgcaacatc catcgcggcg
     1141 gtcgcggctt caatctgcgc ttcctggaag taatatggcg cttgcatcag catggcgact
     1201 ttgtaaggac gaggatacgt ctggcactca aaacgaacgg cgtgtgcaat ggcgcgacaa
     1261 atgtcctgct ccacaacttg caaagacatc gccgcgtagt tttcactcat ggcctgcgtt
     1321 gagtaatagt aatcatcctg acttcccgtc atacaagcga tatccgcgta gtctccgttc
     1381 tgcttaatgc tttccagcaa aactgacagt tcctcttctg caaccgaata gggtggcgag
     1441 agaaacacgg tgcgcgccac cagctgccca gccgcagaat gctggcgaat gaactcagcc
     1501 aggatttcag caggttctgg ttcggttacc gtatcaacgg ggtgacgcat cccctgcaac
     1561 cattgccgct tcgcctcgtc aacttcgctg actttcgcta acggcaattc atcctgatgg
     1621 ttcatcttac ccattcctta cgcaggcagt agattgtgtg ctgccagatc ggcggcaata
     1681 tcttccagcc ccagacgctg caatgtttcg cgggttggac aaccaagctg tgggtcccag
     1741 cccatttctt tgtagaacat cgtgagcgaa gcatgcatat cgtcacgatc cattttgtca
     1801 gtaccttcag taaagaccgg gatctgcgga tccttgtcga aaacccagga acagataaga
     1861 tcgtgttcat tacgcatatc tttggtttgc atcggtttta ccgtgtaggc acgatgcaac
     1921 gtaaagatac gctctgcagc taaatctaat ttttcctgag tcatctcttc gccggtgatc
     1981 gctttgaaga atttggcttc aagcgccaga tcgccccggt aattacggct tttcagcggc
     2041 gaaacggtca ttggccagac ccagttgcac agggtgacgg cgttatgcaa acagacccgc
     2101 aacagcgacc actttgcata tttaattttt gcgtcattga ttggcgtgta gtttttggtt
     2161 tcatcgtaag catcttcaga accaaaaagt tctttcgcca cttcacgttg cagtttcaat
     2221 ggcaagccgg aaccaataaa gttgatatgg gtatgcgtca tgcagtcacg gttgaacata
     2281 cagttaacaa tggaaccgac ctgcgctgac gcttcattgg catggtgaac cggatagcca
     2341 aacggcgacc agagtttatt tttcgcgtag ccccagtact ctttacccaa attccagcgt
     2401 tctgcgatgg catatgaacc ataagccagg tgactcagct cacccacacg atgcgccaga
     2461 cggtagtaaa aatctttaat gaagttaacg tcacccgctt ccagttgatc ccagcgaatt
     2521 tctgcatact cttcagctgg cagaacacgc ttgaacacac ctttgctgta acaataagta
     2581 aagtcgcgat gcaactgccc gtagttacac cataagccgt agtcatcgaa caaattcaga
     2641 ccaaccaggt taccaatcac acgaccatcg tctttatctt caaaatcttt cggcccgttc
     2701 gggaagatgg tggtatggac aaagtttgct acacaagtgt taccacctgt gctgggcacg
     2761 ccaaactctt tcacccgagg aatattcatt tgggtcatac aacggatcgg gcaagagtgg
     2821 cagccgctca tttttactgt gtatttctct gccgccggtc ctaagtcaaa aacggattta
     2881 taggtacgaa agccgaccgt attctgattg cctggcggaa tttcacccgt ttcaatcgga
     2941 ccaccttcag ccgcgcccca aaacagccct ttacgtgctg tccagcgtga cttgggatct
     3001 gaatactctg cccacgattg tggcgtactt ggcacgacat ggttgttatt cgcaccaata
     3061 agttcagtca tcatgtaatc attgagacgc ttcatctcct gacgatcggc aatgttgacc
     3121 cctttcgtcc cttcaaccgc aatcgctttc aggtttttcg aacccattat tgcgccagtt
     3181 cccgcaccgc cgctgtggtt acggctattc aacatgccag agaga
//
LOCUS       DAJTYG010000207         3213 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTYG010000207 DAJTYG010000000
VERSION     DAJTYG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.209"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..410
                     /locus_tag="OO568_004003"
                     /pseudo
     CDS             1..410
                     /locus_tag="OO568_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            994..1710
                     /locus_tag="OO568_004004"
     CDS             994..1710
                     /locus_tag="OO568_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643106.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGVLFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVRKHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2262..2666
                     /locus_tag="OO568_004005"
     CDS             2262..2666
                     /locus_tag="OO568_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643107.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2729..3213
                     /locus_tag="OO568_004006"
                     /pseudo
     CDS             2729..3213
                     /locus_tag="OO568_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          908 a          651 c          735 g          919 t
ORIGIN      
        1 gaagacccag atatgaacag gcacattcag agttacttta aagagcttac tgaacgtctg
       61 aggtgttaga gccatgactg ccgcagcatt gctggcggaa atgcctgaat cgagcagcct
      121 ctcgagacgt gaaatcagcg cacttgtcgg tgttgctcag gtaaaccggg actcgggaac
      181 tctgcgcggt cgacgaacca ttttcggtga ttgtgctggg gaagaacagc tctgtacatg
      241 gcggcgcttg aggcccccgg tttaatctgg tgataaaggc gttttacatg cgcctacttg
      301 cagccggaaa cgccaaaaaa gtggcactgg ttgcctgtat gcgtaagttg ctgaccatta
      361 tgaacgcaat gctcagaaaa aacgaagaat ggaatgaatc gtatttatga aaactggatt
      421 gtgggccata aatattccaa aaagatagcc cgctccgttt attaccggaa cgggcgagag
      481 catgacattg tctgttatta taatgagact gctgattcta cttattttta atttcaagaa
      541 aagcgttttc tacaaagaat tatgagattt aatgttttgt ttagacaata ttatcattct
      601 gttcaataat gaaagtcttc atcaacaaca agtaaaaaca acatccctct catcgaaccg
      661 gacttgcaga tttcccgcat ccggctctct tgcaacacat cagaccttca cgttcgggcg
      721 gttatgggga aggtccagca agctgtatga gccctaactg tttgaatacc agtagattac
      781 cagaaacttc tatcgattga agacattacc aactagcgtt actaaatgtt gaccaagttt
      841 tgttcacttg tctggttttg atagtagcat tctgcagagt attttgatat gataaatatg
      901 cattaacaat gatattattg ttaatttcct agcataaaaa ttaaaagatc aacttttatc
      961 tcgaatattt tttacattat cagttggttt cttatgaatg acaattcatt gcttagaaat
     1021 tcttcattat tcatcgccta tatgggatgt gtagggtggg tcagtgctta ttcttatggc
     1081 tggggaacct ctttttacta tggatttcca tggtgggttg ttggtgcagg tttggatgat
     1141 gttgccagaa gtttactcta tgctatcata gtaatgggta tactttttac cggttggggg
     1201 attggtgttt tattttttct tcttataaag aaaagaagta aaatacaaga cttgagtttt
     1261 tttagactat tttttgctat taccctactg tttttccctg taatattcga acttttaatt
     1321 ctcaaacaat atttcatact tccattatca ctttcatgca ttattagttc attggttatt
     1381 tcaataataa ttcgtatata tggacggatc ttttctgtat catgtttttc agatatccct
     1441 tttgttagaa aacaccgcat taagttaatt atggctggtt ttttggttta tttttggctt
     1501 ttttcctttc ttgttggctg gtataaacct caattaaaaa aagaatatca gatgctttgt
     1561 tataataata gttggtatta tgttcttgct cgttatgatt cacggctcgt tttgtctagt
     1621 tcatttaaag atgacagtaa cagattctta atatttaata cagaacagtc gggattttat
     1681 gaaatcaatg atgtttatgt cagaaagtaa taatcctctc ccacaaagtg aattaaatga
     1741 atatataatg cgttcgaagt atatgtattg agctgtcata ctgagaaaca aaccttaggg
     1801 cagcacaatt aaagccagaa acaactatgc aagaaagtta ctaaataagt gctcggaaca
     1861 gtaaaacact caggggggac tcagtccgga ttatgtaatc agataaattg ccaaacatca
     1921 caaatcacca gccggactga gtgatgccaa gcattgcacc aataccccgt gatgaacgat
     1981 gcctgatgca gaaagctccc tataaaacaa agaacaaaaa ctacgaccgg cgattcaccg
     2041 tcatactgat gctgcatcgt ggtgacactg tctgtcactt tcatgttgac tgaacaactc
     2101 tgctgcgaag aaggtgtacc gccggcggga tttgattgtc cggcgtctag cttccacttc
     2161 cctgatggaa ctgaaagttg ttatggccgg tcagcttgcc ctgtccgcag gacatgaggt
     2221 gctgtgacgg acagcaccag taacggaaag tgacaatgaa aatgaatact gcgctgatag
     2281 ttgcactaat gtgtatgtgg tatgcggtac ccgctgctgc aaaagagact ttactggcta
     2341 tgccacgtaa ttcaacagaa cattgctatg cggagattaa tgtgcacgga ccatatgggg
     2401 tgtatttccg agtggtcccg catccccccg ggggaaagag ctgggtcgaa tgtaacagtg
     2461 actactatta ctctgataaa ccaccgggag tacagatact cggaacccgt gcagggtgtc
     2521 gggtatacgg aatctgcggg accacctcga cactgcatgt ggctggaaga ggggttgtgt
     2581 gtattaaaaa tatatgcagt ccacgtggga tgataattca caggatacgg aaacgtcctg
     2641 tagtggctgt aagcgatgag atgtgatctt acccagcaat agtggacacg cggctaagtg
     2701 agtaaactct cagtcagagg tgactcacat gacaaaaaca gtatcaacca gcaaaaaaac
     2761 acgtaaacag cattcgcctg aattttgcag tgaagccctg aagcttgctg aacgcatcgg
     2821 tgttgctgcc gcagcccgtg aactcagcct gtatgaatct cagctctatg cgtggcgcag
     2881 caaactgcag caacagatga cctcgtctga gcgtgaaagt gaactggccg ctgaaaatgc
     2941 ccgcctgaag cggcaactgg cggagcaggc agaggaactg gctatcctcc aaaaggccgc
     3001 gacatacttt gcgaagcgcc tgaaatgaag tatgtcttta ttgaaaaaca tcaggctgag
     3061 ttcagcatca aggcaatgtg ccgcgtgctc cgggtggccc gcagcggctg gtatacgtgg
     3121 tgtcagcggc ggacaggaat aagccctcgt cagcagttcc gtcagcactg cgacagcgtt
     3181 gtcctcgctg cggcctttac ccggtcaaaa cag
//
LOCUS       DAJTYG010000208         3212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTYG010000208 DAJTYG010000000
VERSION     DAJTYG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.210"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..2126
                     /locus_tag="OO568_004007"
     CDS             <1..2126
                     /locus_tag="OO568_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6643108.1"
                     /translation="KTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVL
                     AVNSQTRTLTLDREITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVA
                     EYSVWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGT
                     VNGVTPPAVQHLTAEVTADSGEYQVLERWDTPKVVKGVSFLLRLTVAADDGSERLVST
                     ARTTETTYRFRQLALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQ
                     ITVVPKLAVYDPTVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYY
                     FYVRSVNTVGKSAFVEAVGRASDYAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDL
                     TEIRTSITNVSNEITQTVNKTLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGR
                     LYIAGIGAGIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQSDQIFMNDVFLKR
                     LTAPTITSGGNPPAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSA
                     NQIEGDLVKTVGKPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHER
                     KNNNIYSSCRLIVKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNG
                     WYPTASISDLLVVVMKKATAGITIS"
     gene            2194..2793
                     /locus_tag="OO568_004008"
     CDS             2194..2793
                     /locus_tag="OO568_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6643109.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            2815..>3212
                     /locus_tag="OO568_004009"
     CDS             2815..>3212
                     /locus_tag="OO568_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6643110.1"
                     /translation="MTARWFRRAFCGVNMAVKISGVLKDGAGKPVVNCAIELRARRTS
                     PTVVAHVVATCVTDNNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNA
                     FLNAPKDGDLRPEVMKRFEEMVAQAQQSAGA"
BASE COUNT          759 a          759 c          993 g          701 t
ORIGIN      
        1 ttaaaacgga actgctggaa acgcagaccg tggacttcag cgtcggcgca gaagggcttc
       61 gccatgtacc gggtgatgtc attgaaatct gcgatgatga ttatgccggt atcagcaccg
      121 gtggtcgcgt actggcggtg aacagccaga cccggacgct gacgctcgac cgtgaaatca
      181 cgctgccgtc ctccggtacc acgctgataa gcctggttga cggaaatggc aatccggtca
      241 gcgtggaggt tcagtccgtc accgacggcg tgaaggtgaa agtgagccgg gttcctgacg
      301 gcgttgcaga atacagcgtg tgggggctga agctgccgac gctgcgccag cgcctgttcc
      361 gctgcgtgag tatccgtgag aacgacgacg gcacgtatgc catcaccgcc gtgcagcatg
      421 tgccggaaaa agaggccatc gtggataacg gggcacactt tgacggtgac cggcgcggca
      481 cggtgaatgg tgtcacgccg ccagcggtgc agcatctgac cgccgaagtc accgcagaca
      541 gcggggaata tcaggtgctg gagcgatggg acacgccgaa ggtggtgaag ggtgtgagct
      601 tcctgcttcg cctgaccgtg gcagcggacg acggcagtga gcggctggtc agcacagcca
      661 ggacgacgga aaccacatac cgcttcaggc agctggcgct ggggaattac agtctgacag
      721 tccgggcggt aaatgcccgg gggcagcagg gcgatccggc gtcggtatcg ttccggattg
      781 cggcaccggc agcgcctgtc actattgaac tgataccggg gtattttcag ataacggtgg
      841 tcccgaaact ggctgtatat gacccgacgg tgcagtttga gttctggttt tcggaaaagc
      901 ggattgcgga tatcaggcag gttgaaacca gcgcccgcta tcttggcacg gcgctgtact
      961 ggatagccgc cagtatcaat atcaggccgg gccatgatta ttatttttac gttcgcagtg
     1021 tgaacaccgt tggcaaatcg gcatttgtgg aggccgtcgg tcgggcgagc gattatgcgg
     1081 aaggttatct gagtttttat aaagggttga tcaataaaac gcatctcggc aaggagttgt
     1141 ggacgcagat tgataacggt cagcttgcgc cggacctgac tgaaatcagg acgtccatta
     1201 cgaatgtcag caatgaaatc acgcaaaccg tcaataaaac actggaaaat cagagtgctg
     1261 caatccagca gatacagaaa gttcaggttg atacaaataa taacctgaac agcatgtggg
     1321 ctgtgaagct gcaacagatg aaggacggac gcctttatat tgcgggtatc ggtgccggta
     1381 ttgagaatac gccagcagga atgcagagtc aggtgctgct ggcggcagac aggattgcga
     1441 tgattaatcc tgcgaatggc aacacaaagc cgatgtttgt tggtcagagc gatcagatat
     1501 tcatgaacga cgtgttcctg aaacgcctga cggctccgac cattaccagc ggcggtaatc
     1561 ctccggcatt ttccctgaca cctggcggac ggctgacggc gaaaaatgcc gatatcagcg
     1621 gtaacgtgaa cgcgaactcc gggacgctca acaacgtcac gattaataag aactgtcggg
     1681 ttctgggaaa attgtccgcg aaccagattg aaggcgatct cgttaaaaca gtgggcaaac
     1741 ctttcccacg ggactcccgg gcaccggaga ggtggccatc agggaccatt accgtcaggg
     1801 tttatgacga tcagccgttt gatcggcaaa ttgttattcc cgcggtggcg tttcgcggtg
     1861 ctaaacatga gcggaagaat aacaatattt attcgtcatg ccgcctgata gtgaagaaaa
     1921 acggtgctga aatttataac cgaacgaccc tggataatac gctgatatat acgggtgtta
     1981 ttgatatgcc tgccggtcac ggtcacatga cgctggagtt ttctgtatcg gcatggctgg
     2041 taaatggctg gtatcccaca gcaagtatca gcgatttgct ggttgttgtg atgaagaaag
     2101 ccactgcagg catcacgatt agctgaattt tataacccag atacgggcac cagaaatggt
     2161 gcctttttta ttgcagaaaa gcgagaggta attatgcgta aagtttgtgc agccattttg
     2221 tccgcagcca tctgtctggc tgtatccggt gcgcctgcat gggcgtctga acatcagtcc
     2281 acgctgagcg ccgggtatct tcagccccat actgatatgc caggcagcga tgacctgaag
     2341 ggcattaacg tgaaataccg ttatgagttt acggacacgc tggggctggt gacgtcattc
     2401 agctatgcca acgctgaaga tgagcaaaaa acgcattaca gcgatacccg ctggcatgag
     2461 gattccgtgc gtaaccgctg gttcagcgtg atggcggggc cgtctgtgcg cgtgaatgaa
     2521 tggttcagcg cgtatgcgat ggcgggcgtg gcttacagcc gtgtgtcgac cttctccggg
     2581 gattatctcc gcgtaactga taacaagggg aaaacgcacg atgtgctgac cgggagtgat
     2641 gacaaccgcc acagcaacac gtctctggcg tggggggctg gcgtgcagtt taacccgacc
     2701 gaatccgtgg ccattgacct tgcttatgaa ggttccggca gtggcgactg gcgcactgac
     2761 ggtttcattg tgggtgtcgg ttataagttc tgattagcca ggtaacacag tgttatgaca
     2821 gcccgctggt tcaggcgggc tttttgtggg gtgaatatgg cagtaaagat ttcaggtgta
     2881 ctgaaagatg gggcgggtaa acctgtcgta aattgtgcga ttgaactgcg ggccagaaga
     2941 accagtccga ccgttgtggc acacgttgtt gccacttgcg tgacggacaa taacggtgct
     3001 tatgtgattg aggctgagcc ggggtattac gaggttgcgc ttcactgtaa cggctggcag
     3061 ccaacccgtg tcggggatat tgatgtggca ccgactgatg caccggggac actgaacgcg
     3121 tttctgaatg caccaaagga tggtgattta cgtccggagg tgatgaagcg ctttgaggaa
     3181 atggtggcgc aggcgcagca gagtgccggg gc
//
LOCUS       DAJTYG010000209         3175 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTYG010000209 DAJTYG010000000
VERSION     DAJTYG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.211"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="OO568_004010"
     CDS             complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="OO568_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6643111.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQ"
     gene            complement(1002..1097)
                     /locus_tag="OO568_004011"
                     /pseudo
     CDS             complement(1002..1097)
                     /locus_tag="OO568_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="OO568_004012"
     CDS             complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="OO568_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR6643112.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="OO568_004013"
     CDS             complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="OO568_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR6643113.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            1952..2092
                     /locus_tag="OO568_004014"
     CDS             1952..2092
                     /locus_tag="OO568_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643114.1"
                     /translation="MVPEAGSEVLSRVIASGREVLTRVLVTGDEVLTRVLVTGDEVLP
                     RV"
     gene            complement(2227..3175)
                     /locus_tag="OO568_004015"
                     /pseudo
     CDS             complement(2227..3175)
                     /locus_tag="OO568_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          788 a          766 c          774 g          847 t
ORIGIN      
        1 tgttgtccgt cactcatgga gaagtaaatc cgtgggccgt ggtagtccgg cgaagaggtt
       61 aatctttgca gggcttgcag agcatgggat gataatgggt tatcactaat atgtattgaa
      121 cattcattcc tcagattaag aatgctttcc gggatatgac ttatctgatt tctatcaaaa
      181 aaatactctc tgacaacggg attatttccc tcagaaaatg ctggtagctc agtgaggaaa
      241 ttacgggtcg ctctgagatt tttaagagcc tgtgggagcg atggtaagga gactatttcg
      301 ttatagctga tattcatagt caataacgaa tcgggtaaag ctggcaggtt ttccagctca
      361 ttgtgttgta cctgaagtga ctccagcgca ggaggtagca cgggcaattc tgataattta
      421 ttgctggcgg aatataattt ttttagcgta acaggcagtt caggtaggtt agttaattga
      481 ttatatgata cattgagcag cgttatctga gctggtaagt tgtcaggtag cgaggacaga
      541 tttaaacggt ccagtcgaag ttcatcggaa ttattgataa gacattcttt aagtcgggag
      601 acagcctcat cacgctcttc accggggagc gcttgttttt cccatttatc ccatgctgaa
      661 aagtaatcag catatgtacc ggaaatagtg ttataaaaag aattttgggg caatgaaaag
      721 ttattattta tcggtaacat gatgggcatc cagttaaagc cggtggacaa atggctttgt
      781 taaaagaaat aagtaggagt ttttatgaag ttcattccat gaaatgaact tcagcacttt
      841 tttattataa taaggataaa caaaattggc tgtaaaaaaa cgatactgtt tcagttaacc
      901 gtgaatggcc tgtttgcacc gtattgttct tgtttttcgg ttagttaaac atcgtacttc
      961 atatttgaac ggtaagcggc tcgtcagaac cgtattgata tttactgaga ggtcagatca
     1021 actttccagg gcaacagatc gcgtacccgg tttgccggcc agtcctggat atgctcaatg
     1081 acgtaacgca gccattttgt caacgccacg atttttgacc ctagcctgac atttatattc
     1141 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     1201 cttcttcccc gataacgaag atcggcacac tggccatatc agtggtcatc atgcgccagc
     1261 tttcatcccc gacatgcacc accgggtaaa gctcacggga gactttatct gacagcaggc
     1321 gggcactggc cagggggatc accatccgtc gtccgggcgt gtcaataatg tcactctgta
     1381 catccacaaa cagacgataa cgactctctc ttttataggc gtaaacctta aactgcattt
     1441 caccagtccc tattctcatc ggcaaaagag ccgttcattt caataaaacg ggcgatctca
     1501 gccatcccct gctgattttc agcctgccag cgttccgcac gcagacggcg ggcctctttt
     1561 tgcatggccg tatttaccag gcctgagata ttcacgtttg cggatttaag taactgatag
     1621 ctgtcactgt ctatagtgac agtaatacgc tgcttcatga taagccctca aaaacatact
     1681 tatgatatgt atatgagtat atattaatgg tacaaaccat caatatccat aaaaagtaca
     1741 ccattaattc tctataagtt tttaatacaa ttttttctgc tgcgattttt ttatactata
     1801 attagcatac ttataacacc gtaagtaaat tacatgaaag caggcacagc gtgatccgat
     1861 ttttctgcgt ataacgattt tccggaagtg taatacggtt gcgccccggg ccgggcaccc
     1921 ttttctgttg ttgtgtacgg gcccacagag aatggtaccg gaggcgggct cagaagtact
     1981 gagtcgggtg atcgcgtcag gccgcgaagt actgacacgg gtattggtga ctggtgatga
     2041 agtactgaca cgggtattgg tgactggtga tgaagtactg ccccgggtat gatttcaggc
     2101 tgattcaggt tctgattctc tgtcagtccg cgtggttgct ggcctggctg atttcctgtt
     2161 gtcaggcgtt caacaaccga ctgcaggtta tagcccggtt cacgaacacc tgacggcagg
     2221 tatcactcac ccggtgaagg taaatccctc aagaggcagc aactctgcca gtcgctcctc
     2281 cggccattcc ggcagacgcg tcaggacgtc cgtcagccag gcatgtggct ccagaccatt
     2341 acgttttgct gtttccagaa ggctcattat ctgtgccgcc cggtttccgg ccatcagtga
     2401 accggcgaac agccacgctt tccgtcccat aacaaccggg cgaatggcac gctcgcagat
     2461 attgttgtcc aggggcaccg ctccgtcacc caggaagcag ctcagttcat cgcgccggtt
     2521 cagaatatag ttaatcgcct tatttaatgg cccgtcgggc ggacagcacg gctcctgctc
     2581 ttcaagccag gcaaacaggt cattcactat cggccgggag taccgttatc gccactgccg
     2641 tattttttcc acagggcgct cacggatgag tttctcaatg cggtaaagac ccgcgatttt
     2701 tctcagctcc acgcctgcac ggggctccct gctggcctta tacagatttg cgaagccgcg
     2761 ccctgcgtga ctccagcaac aggctgatgt gatgccgggg tgattatccg ccagagattt
     2821 gtacacgctg tatccgtcac tcaccagcgt accgccacac cagcactgca gccaggtctc
     2881 agggtatctg agggcccgtc cggtctgcga gtcaaaacag acgaccggcg gaccgcttct
     2941 ttcaccgctg acgtaagcac acagccagcc ggagcaggat ttaccgcctt tccgggtatc
     3001 aaggagcctc agggacgttt catctgcatg aataacatcc cgtgtcagaa gctcatggcg
     3061 tagcagtttc gccagcgggg ccagtgcagc tccggcaaca ccgaccatat ccgccattgt
     3121 actgacgggg agctccacac ccattcggga gaaaatgtgc tgctggcgat acagg
//
LOCUS       DAJTYG010000210         3145 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTYG010000210 DAJTYG010000000
VERSION     DAJTYG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.212"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            845..1009
                     /locus_tag="OO568_004016"
     CDS             845..1009
                     /locus_tag="OO568_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6643115.1"
                     /translation="MARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSIQI
                     RFEAESETGT"
     gene            complement(2034..3143)
                     /gene="fepE"
                     /locus_tag="OO568_004017"
     CDS             complement(2034..3143)
                     /gene="fepE"
                     /locus_tag="OO568_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6643116.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          933 a          780 c          652 g          780 t
ORIGIN      
        1 aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta ggaaaaaacc
       61 taaaaaccat tcagcagaca tttaccataa catctcattg cgcgcaccgc taaaacgcgc
      121 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
      181 aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg aatgacgatc
      241 ggtttgggcg catacagtgc ccatgagaca cattttcgga cattttcagt tcgtgaatcc
      301 ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg ggcgtcctgt
      361 aaaaaaacac accgcgccgc tagcagcccc cccctaaaaa gcggtgttgg cgcagctaca
      421 acaccgcgcc gacaccgctt ttttaaatat cacaaagaga gcaagaagaa ctaatttgtc
      481 ataactctct atttataaag aaaaatcagc aaaaacttgt ttttgcgtgg ggtgtgggca
      541 tttcaggcat gagaacagac tcgcgttttt ccggagtatc agcggaatta ttgattaaga
      601 aacactcgac gcagagcgaa attttatgca acagcggctg cactgcacag ggattattta
      661 tcagttgata tgctgtgcca tgatacgtcc taaagaaaca ggacaggatg tgcaattgta
      721 ataccgtcgc ccgcaacgtc ttacaattac cacctgctca gggccccccc cccgacaccc
      781 ggaactgacg gagaacaacg gaaggtgaac aaacaacaac aaacaacaac aaactgcgct
      841 gaatatggcg cgatgtatca aaagccagag cctgacactg cttgaaaaac tggatgctct
      901 ggatgccgac gaacaggcgg ccatgtgcaa acgactgcac gaactcgcag aagaactcca
      961 gaacagcatc cagattcgct ttgaggccga aagtgaaaca ggaacataac gaagctgacg
     1021 gaggcggtca cggctttccc gtgggcgttt tcgtcagcag acaagcccgt cagggcgcgt
     1081 cagggggttt tagcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     1141 tatatactgg cttaatcatg cggcggcaga gcagatggtg agagaacagc gacatatgag
     1201 gtgtgaaatg ctgcacagat acgtaaggag aaaatgcagc ttccggcgct cttccgcttc
     1261 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtgt ctgctcacgc
     1321 aaagacggat atacggttat ccacagaatc aggggataac accagaaaaa acatgtgagc
     1381 aaaaaatagg ccccgaaaaa aggccgcgcc ggtggcgttt ttccataggc tccgcccccc
     1441 tgacaagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggacttaa
     1501 agataccagg cgtttccccc tggtggctcc ctcgtgcgct ctcctgttcc tgcctttcgg
     1561 tttaccggtg tcattccgct gttatggccg cgttcgtctc attccacgcc tgacactcag
     1621 ttccgggtag gcagttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1681 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttaac
     1741 gccattggca gcggccactg gtaaacagga tgtgcgagag atgtattgag agttcttgaa
     1801 gtggcagcct tactgaggct acactagaag gacagatttg gtgacatgtc tcgcacaaga
     1861 ctgttaccac ggttcagaag ttccccaact tactgaacct tcgaaaaacc acctccccag
     1921 gtggtttttt cgttttctag agtagaagac tacggctgta agggaatgat ctcaagaaga
     1981 tcctttgaac tttttataaa agttgcttat aaaatgatgc atcagcacaa cttttactca
     2041 atcacactca tctttctggc ctccatggca tgacgtaaaa gtatatagcc acatgcaagc
     2101 atccctccaa tgacagaaga aagaattaca attatggcat tacttggccc atcctttctg
     2161 accggtaacg ttgggctcaa ttgatactga aatggcataa aatcaacatc ccccactttc
     2221 gccacaacca attgattcac aacatattga cgatttcgca actccccact caattccgaa
     2281 acatccttga ttgatttttt gatgttcaat ttagttgcta taccatcaga accgagagcc
     2341 acaggaaaat ctgggtcatc cttcataatc tgaccattgc tgtatacagg tttttttatt
     2401 ccagccgcat tagcaacctc cagtgaataa ttgaggcgtc gaatatctgc attcagctgg
     2461 tttctaattt ttatctcatc cagtgcaaga atttcttttt caacattagt tttaacttct
     2521 agtttatttc ttatatcttc catcaaatcc cttacaacaa gggaagaaac ataattaata
     2581 tacccagaca acacatcgtg agcttcttta cttgttggag cagtaaaact cagagtccat
     2641 gatacataca atgatgactt gtcgtttttc ttactggcat tatcatcaat cgacttcata
     2701 tttttactca actcaacaat tgcacgatgc agttcgagag cgctaatgtt attcttttta
     2761 ataatctcca taacataagg agatgatctc atatactcat tcaacgaatt aacggactga
     2821 aatttcttaa taaacagagc aaatgcgcta tcacgattga tatcaaaatc cactcccaga
     2881 acatgaagct tggaaagctc cttatttaat tcactccatt gaacagactc agcaggagta
     2941 accacagcag aactagtcca tttttgtggc aaaacaaaag aaacaagaaa gccaatacaa
     3001 gcaaaaaata gaatcacccc aacaattttt atttttgctg cccataaaac gctaatcaag
     3061 ccaaaaaggt caatttcttc attaccattc ggcaacatgt tgtaattaga aaaatgttcg
     3121 tcagagacct ttgagatttt cattg
//
LOCUS       DAJTYG010000211         3060 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTYG010000211 DAJTYG010000000
VERSION     DAJTYG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.213"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            238..2106
                     /gene="kup"
                     /locus_tag="OO568_004018"
     CDS             238..2106
                     /gene="kup"
                     /locus_tag="OO568_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6643117.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2273..2692
                     /gene="rbsD"
                     /locus_tag="OO568_004019"
     CDS             2273..2692
                     /gene="rbsD"
                     /locus_tag="OO568_004019"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6643118.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2700..>3060
                     /locus_tag="OO568_004020"
     CDS             2700..>3060
                     /locus_tag="OO568_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6643119.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYV"
BASE COUNT          689 a          730 c          807 g          834 t
ORIGIN      
        1 gaagggcctt cccgagagtg gtaaaggaga ggccctggaa ataccccaca gccccaaaaa
       61 agagccagtc agttgcttaa ctgactggca ttactcaaag ggggggcttt ttctttgagc
      121 caggttaatg tggccgcatt taggagtacg attttgccgt taatcgtgca tactgtgcgc
      181 ttttttgtgg gccaagggac taagcacaca tttcatattt caacgaaaga ctagtctatg
      241 agcactgata ataagcaatc attgcccgcg attaccctcg cggcgattgg agttgtctac
      301 ggcgatattg gtaccagccc gttatataca cttcgtgaat gtttgtccgg ccagtttggt
      361 tttggcgttg aacgcgatgc cgtgtttggc tttttatcgc tgatcttctg gctgctaatc
      421 tttgtggttt ccattaaata tctcaccttc gtgatgcggg cagataacgc cggtgagggg
      481 gggatcctga cgttgatgtc gcttgccggg cgtaatacgt cggcgcgaac cacatcaatg
      541 ctggtgatta tggggctaat cggcggcagc tttttctatg gtgaagtcgt cataacaccc
      601 gctatttcgg tgatgtcagc cattgaaggt ctggaaatcg tcgccccgca gctggatacc
      661 tggatagttc ccctctcaat tatcgttctc actttattat ttatgattca aaagcatggc
      721 acggctatgg tcggtaagct gtttgcgccg atcatgctga cctggttttt gattctggca
      781 ggactggggt tacgtagcat tattgctaac ccggaagtgc tgcatgcgct gaatccgatg
      841 tgggcggtgc atttcttcct tgaatacaaa acggtttctt ttattgcatt aggggcagtg
      901 gtgctgtcga ttacgggggt cgaggcgctg tatgctgata tggggcactt tggtaagttc
      961 cctattcgcc tggcgtggtt caccgtcgta ttaccttcct tgacccttaa ttacttcggt
     1021 cagggagcgt tgttgttaaa gaacccggaa gcgattaaga acccgttctt cctgttggca
     1081 ccggactggg cgctgatccc actgctgatc atcgccgcac tggcgacggt aattgcctcg
     1141 caggcggtta tctctggcgt cttctcattg acgcgtcagg cggtacgtct gggatatttg
     1201 tcgccgatgc gcattattca cacctccgaa atggagtcag ggcaaatcta tattccgttt
     1261 gtgaactgga tgctctatgt cgcggtcgtg attgtgattg tcagctttga gcactccagc
     1321 aacctggcgg cggcgtacgg gattgcggtg accggaacca tggtactgac gtctattctc
     1381 tcgactaccg tggcacgtca gaactggcac tggaataagt attttgttgc gctgatcctg
     1441 attgctttcc tttgtgtcga tattccattg ttcaccgcta acctcgataa actgctctcc
     1501 ggcggctggt tgccattgag cctcggtact gtgatgttta tcgtgatgac cacctggaag
     1561 agcgagcgtt tccgcttgct gcggcggatg catgaacatg gtaactctct ggaagcgatg
     1621 attgcttcgc tggagaaatc accgcctgtt cgcgtgcccg ggaccgcggt gtatatgtcg
     1681 cgtgcaatca acgtcattcc ctttgcgctg atgcataacc ttaaacataa caaggtattg
     1741 catgagcggg tgattttgtt aactctgcgc accgaagacg cgccatatgt ccataacgtc
     1801 cgtcgggtac agattgaaca actgtcgccc actttctggc gcgtggtggc aagttatggt
     1861 tggcgagaaa cgccaaacgt ggaagaagtt ttccaccgct gcggtctgga aggattaagt
     1921 tgccggatga tggaaacctc cttctttatg tcgcatgagt cgttgatcct cggcaaacgc
     1981 ccgtggtatt tgcgtctgcg cggcaagctg tacttgctgc tgcaacgtaa tgcgctgcgt
     2041 gcaccagatc aatttgaaat cccgccaaac agggttatcg aactgggtac tcaggtcgaa
     2101 atctaacgcc agacgcctcc tttcttcata agggggcgtt tttgttttca tggttaatca
     2161 ccatgtaaaa cgtttcgagg ttgatcacat ttccgtaacg tcacgatggt tttcccaact
     2221 cagtcaggat taagctgcgg atcagcgaaa cgtttcgctg atggagaaaa aaatgaaaaa
     2281 aggcaccgtt cttaattctg atatttcatc ggtgatctcc cgtctgggac ataccgatac
     2341 gctggtggtg tgtgatgctg gtttacccat ccccaaaagt acaacgcgta tcgatatggc
     2401 attaacccaa ggtgtacctt cttttatgca ggtgctgggc gtcgtcacaa atgaaatgca
     2461 ggtcgaggct gccattatcg cggaagagat caaacaacat aatccgcaac tccacgaaac
     2521 gttgctcact caccttgagc agctgcaaaa acaccaggga aataccattg aaattcgtta
     2581 caccacgcat gaacaattca aacaacaaac cgcagaaagt caggcggtaa ttcgcagcgg
     2641 agaatgttct ccgtatgcga atatcattct ctgtgctggc gtgacgttct gaggccgtca
     2701 tggaagcatt acttcagctt aaaggcatcg ataaagcctt cccgggtgta aaagccctct
     2761 cgggcgcagc gttaaatgtc tatccgggcc gtgtgatggc gctggtgggc gaaaacggcg
     2821 cgggtaaatc caccatgatg aaagtgctta ctggtatcta tactcgcgat gccggcacgc
     2881 ttttatggct ggggaaagaa acgacattta ccgggccgaa atcttcccag gaagccggga
     2941 ttgggattat ccatcaggag ctgaacctga tcccgcagtt gaccattgcc gaaaacattt
     3001 tcctcggtcg tgagtttgtt aatcgctttg gcaaaattga ctggaaaacc atgtatgtcg
//
LOCUS       DAJTYG010000212         3050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTYG010000212 DAJTYG010000000
VERSION     DAJTYG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.214"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO568_004021"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO568_004021"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6643120.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLLGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO568_004022"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO568_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6643121.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..>3050)
                     /gene="bcsE"
                     /locus_tag="OO568_004023"
     CDS             complement(1963..>3050)
                     /gene="bcsE"
                     /locus_tag="OO568_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6643122.1"
                     /translation="EEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQN
                     GIWTLVQSEEAEIQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAA
                     TVVFSLQQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVI
                     PWNAPLSRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMM
                     NNPLLPAHGKGVLVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRIND
                     LDTALNHIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVI
                     NAEHDGRHWRRIPEPMRLLDDAVERSS"
BASE COUNT          763 a          786 c          806 g          695 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aagcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa
//
LOCUS       DAJTYG010000213         3046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTYG010000213 DAJTYG010000000
VERSION     DAJTYG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.215"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..292)
                     /locus_tag="OO568_004024"
     CDS             complement(<1..292)
                     /locus_tag="OO568_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR6643123.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKV"
     gene            1044..1592
                     /locus_tag="OO568_004025"
     CDS             1044..1592
                     /locus_tag="OO568_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6643124.1"
                     /translation="MAVIGYCRVSTQDQTVDNQKLQIEKVHKVDKWFIDEAVSGATKA
                     TSRDGFRNMMNYVRCDDTLVVVAIDRLGRNTVDVLSNVEALQTKGVRVVSLREGFDLL
                     TPIGRAMLTMMAGLADLEKDLISERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQG
                     KTRLQIQEELGISRATYYRLAK"
BASE COUNT          934 a          551 c          687 g          874 t
ORIGIN      
        1 acactttctt aaatggtttc actgaaacgt gttcatagac tcctgccgct acgtacgggt
       61 cagcatcggc ccaggcctga gctgcttcca gcgattcaaa ttcagcaata acggttgagc
      121 cagtaaatcc cgcagcccct ggatcgttac tgtctaccgc tggcattgga ccagctgtca
      181 acaaacgacc ttcatcatgc agtaactgta aacgtgctaa atgtgccgga cgaacggaaa
      241 ggcgtttttc gaggctatca gctttgtctt gagcgtaaat aacatacaac acgggcacaa
      301 ctccttgttc gggaaagttg taaagtacgt tatttgaaag ggcgaagatc tgcaacggaa
      361 agatgatgtc tttgttaagg tcatgcataa agtaagggta attacgccaa aaatgacatt
      421 ttcactgatc ctgatcgtct tgccttattg aatatgattg ctatttgcat ttaaaatcga
      481 ggcctggttt ttctactgaa atgattatga cttcaataac acttgaatga agtctattcg
      541 gttttctagg agacctgtta aaaacaggac aatatcaggt gaatgactat gcaatttaat
      601 agtagatttc caagtatcta agcagtttta cacaaatttt atgcagtatc ggtatagtct
      661 ctttttagca taacttattg aaatatatag agaatctgtg aagtctcttt tcagggaact
      721 atgcagggtg ttagtcgttc ctctctcgac tcctagaaaa gtgctatata cagggctata
      781 taacgatata tagatgtttg gtcacatggt gtaactatca agaaacttaa cgatttccta
      841 cgtgaaagtt agaaagtgat ggttatagtt accctcctaa aatcttcata gctccttcat
      901 aaccagtctt aaaaggactt ccttttgaga cactttcaac gtaacggtag aatgcaccct
      961 caagtagtct caaaaggttg tttttggatt atgagacagc taaacttatg agactaactt
     1021 ttgagacttc taaggggaac attatggcgg tgattggtta ttgcagggta tcaacacaag
     1081 accagactgt agacaaccag aaactacaga tagagaaggt tcacaaggtc gataaatggt
     1141 ttattgatga ggctgtctca ggagctacaa aggccaccag cagagatggt tttagaaaca
     1201 tgatgaacta tgtaaggtgt gacgatacgt tagttgtggt agctattgac cgtttagggc
     1261 gtaataccgt tgatgtgctc tctaacgtgg aggcactaca gactaaaggt gtgagggttg
     1321 tttctctacg tgaaggtttt gacttattaa caccaatcgg tagagcaatg cttactatga
     1381 tggctggttt agctgacctt gaaaaagacc ttatcagtga acgtagaaag gctggtattg
     1441 aacgtgctaa agctgaaggg gtacatatgg ggaggcctgt aaaagcttct tcagaggctg
     1501 tacagacgct tatttcacaa ggtaagacga ggttgcagat tcaggaagaa ttaggaatct
     1561 ctagagcaac ttattacaga cttgctaaat aagttactga atagttacat ggatgtagct
     1621 gatatttggc ttttttgcaa agggggtcac atagctggtg taacaggtac ttagcagcta
     1681 cagacatctg tcagatattt ttgaagattc tcagagaggg aatttgaaga ggggctacta
     1741 tgattttata aaaattttca gagtctgaaa agttactgta cagattttat acaaactaaa
     1801 aaggactccc gtaggtgatt cagtaatgaa ttacttcatg aagagtccct ttacaagtcg
     1861 atttttaatc gtggctacga caatccaccc ataccagacc taaccgtacc ccaccttacc
     1921 aagccatacc ctaccccgcc agactataag gaagcgtaga gaaccgatga aggtgtctgc
     1981 acagttgcat cttaaacaac catcacagtg atgtcaaata gttcttttaa gtaggagggg
     2041 ttcccctatg gtcacatatc ccctctctcc cgcttagtgt cccttgatag tatctagaga
     2101 gtatgcctgt aatgttagag agtatgcctg taatgtttct gtatcaggtc agctataaga
     2161 ggtctggata ttaccttgta agattcttgg tagtgattat gcagagggct tgataagagt
     2221 gtttagcggg aggaagagaa ccatgaaacc gaaggtaggt tctatcttaa cagtgactgt
     2281 aagagatacc tgaaaaaata ctttaacaga actaccaaga agagagggaa cgataagacc
     2341 agaggtagtg acctgttaaa caggctgttt caaagtctct taaaagtctg ttaagagggc
     2401 taggtgaaca atcctaccat tttgtcaagt gttttgtctt taacaaaaag tgctaaactt
     2461 aacaaaaagc actaaatgct aaagagacga ttaagaaata tactaagagg ggcgcttcgc
     2521 ttggggtcac tatgttcttt ctctcccgtt aagaggattt acagggacat ttcaaagccc
     2581 tgataaggta agcccgatga taacagaaac agagggcaaa accacttatc aacactgtga
     2641 acgaataacg taaagggctg gttaagaaag tcggtttagg gattggtaac aataccaatt
     2701 agaagggagc ttcccttcct ggggtgtcgc tctatgggtg aagttaacct ttgaagttct
     2761 ctgtgaagaa actgaacagc tattatattg gctaattaca accctttgaa agataagcat
     2821 ttttagaagt tactgttaag gttgcagagt tgtctgtgaa gagacttgtc agttctctat
     2881 attgggcggt tacaactcat tgaaaaataa gaacttttag aggttactgt cagagaggtg
     2941 aatacttgat agatacagtc ttccggttct gtcaggctat tttttaacag atagcacaaa
     3001 atgctaaaga tgcccctatg ccatctcgta caggggggtt ttgaag
//
LOCUS       DAJTYG010000214         3037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTYG010000214 DAJTYG010000000
VERSION     DAJTYG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.216"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            52..1083
                     /gene="ynaI"
                     /locus_tag="OO568_004026"
     CDS             52..1083
                     /gene="ynaI"
                     /locus_tag="OO568_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6643125.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRERKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(1134..2747)
                     /gene="mppA"
                     /locus_tag="OO568_004027"
     CDS             complement(1134..2747)
                     /gene="mppA"
                     /locus_tag="OO568_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6643126.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT          790 a          705 c          672 g          870 t
ORIGIN      
        1 gccaggggtt tccggcatga cgacaattat ctggaggagc ctgataaaac aatgatcgct
       61 gaactgttta caaataatgc gcttaatctg gtcattattt tcggtagctg cgcagcattg
      121 attctgatga gcttttggtt tcgccgtgga aatcgtgaaa gaaaaggatt tttattccat
      181 gcggtgcaat ttttaatcta caccataatt atcagtgctg ttggtagcat cattaattat
      241 gttatagaaa actataaact caaatttatc accccaggcg ttatcgattt tatctgtacg
      301 tccctgattg cggttattct gacgattaag ctatttctgc tgattaatca gtttgaaaaa
      361 cagcagatta aaaaaggtcg cgatatcacc agtgcgcgga ttatgtcgcg cattatcaaa
      421 atcaccatta ttgtagtgct tgttctgctt tatggcgaac atttcggcat gagcctttct
      481 ggcttgctga cctttggtgg tattggtggt ctggctgtcg gtatggccgg taaagatatt
      541 ctgagtaatt tcttttccgg gatcatgctc tatttcgacc gtcctttcag tattggcgac
      601 tggatccgtt caccggacag aaatatcgaa ggtacagtag cggaaattgg ctggcgaatt
      661 accaaaatta cgacctttga taatcgtcca ttgtacgtac cgaactcgct gttttcgtcg
      721 atcagcgtag aaaacccagg acgaatgacc aaccgccgca ttaccacgac cattggttta
      781 cgttatgagg atgcggcaaa agtgggcgtt attgtcgaag ccgtacgtga gatgctgaaa
      841 aatcacccgg ccatcgacca gcgacaaacc ttactggttt atttcaacca gtttgctgac
      901 tcgtcattga atattatggt ttattgcttt accaaaacca cggtatgggc tgagtggctt
      961 gctgcacagc aagacgttta tttgaagatt atcgatattg tacagtcaca cggcgcggac
     1021 tttgccttcc ccagccagac gctgtatatg gataacatta caccgccgga acagggtcgc
     1081 taaatcagac atcactggcg ttatccacaa gggtaacgcc agttcccatc acatcaatgc
     1141 ttcacaatat acatagtccg actgtacgct acatcttcag ggttattaat gggataacct
     1201 ttcagccacg gtttgattaa tcgtccattg gtatattgat aaattggtgc aatcggtgct
     1261 tgctccatga ggattttttc tgccgcgttg taatcggcat tacgcgcttt aacggtattt
     1321 tccgtcgatg cctgggccag aactttgtcg tatgccggat tgttaaagcg tgaaatattt
     1381 cctgaatgca ttgacgttaa taatgtcagg aaagtggagg gttcattata atcccccacc
     1441 catgaggcgc ggataacatc aaaattgccg gtgttacggc tatcgatata ggttttccat
     1501 tcctgatttt gcaatttaac atctacgcca aggttctttt tccacatcga tgctacagca
     1561 atcgcaattt tttgatggtt ttctgaagtg ttatacaaaa gcgtcagctt cagcggtttt
     1621 tgcggaccat aaccagctgc gctcaacaaa gtttttgcct gcgcattcag ttcttcctga
     1681 ctcatttgtt caaacggcga aggttccggc gtaaatccgg cggtaacatc tggtataaaa
     1741 tgccacgctg gcttttcgcc cgtccccaac actttttcgg tcatcaggcg gcgatctatc
     1801 gtcatactta atgccaggcg aacgcgctga tctgccgtcg gccctttttg cgtgttaaag
     1861 gcataataat aggtcccgag ctgcggtggc gtataaacct gccccggaat atccttcaac
     1921 agcttctgat acatattttt cgggaaggat tcggtgatat caatatcccc cgcgaggtaa
     1981 cgcttagtgg ctgcggattc ctgattaatt ggcaggaagg tcactttttg cagcaccgtt
     2041 ttggcgttat cccaataatg ggtattcggt accacgacca gtttttcatt gactacgcgc
     2101 tctttaagaa cataagcgcc attgccgatc agatttccgg gtttcgtcca ctctttaccg
     2161 ctttctacgt tggctttttg caccgggaag aaggcaaagt tagcggttaa attcacaaac
     2221 cacggcaacg gtttatcaag ctgaattttc aaagtatggg catcaactgc ggtgacgcca
     2281 agctgatcag gcgtagcttt accatcaata atcgcctgtg cgttgttgat tcccgccagc
     2341 gcggcaaacc atgcaaatgg cgacaatgtt tttgggtcca ccagacgttg ccagctgtag
     2401 acaaaatctt gcgccgttac cggtgtgcca tccgcccatt ttgcgttatc gcgcagggta
     2461 aaagtccaga tacggttgtc attacttttc cattgagtcg cgacgccggg gacaatctcc
     2521 cctttttcgt tctgattcac cagaccttca aacagatcgc gaatgacctg aatctctggc
     2581 aggcccacgg ctttagcggg atccagcgac gcaggctcat ctttaatgtg gcgcaccagc
     2641 tcctgcttct ctgccagtac tgtgccgctc ggaacttctg cagcatacga aagagaaatg
     2701 ctgctgacca acagcgcaca acacgtaact gaaacagagt gcttcataag tttccctcta
     2761 accgacatta aaattgatgc gtaattattt gtttcgcctt aaagaaatgc aaacaacttc
     2821 catcaaaaaa gtgacaaagg aagcatcaaa tgttttaagt atcaatatat caaatcagat
     2881 ttagtgtaaa taatagacgc caaaatgcgg caaagcacat agaacaccga acactacgtt
     2941 cgggcttcgc acgttcaaac cagttttcgg tatcataatc agcatctgtc gccattgtac
     3001 gaagaataaa aaataaccaa aacgttgaaa ggaactg
//
LOCUS       DAJTYG010000215         2946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTYG010000215 DAJTYG010000000
VERSION     DAJTYG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.217"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            161..439
                     /locus_tag="OO568_004028"
     CDS             161..439
                     /locus_tag="OO568_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643127.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            441..1499
                     /locus_tag="OO568_004029"
     CDS             441..1499
                     /locus_tag="OO568_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6643128.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNAQVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTSTSILKQR
                     TKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            1500..1865
                     /locus_tag="OO568_004030"
     CDS             1500..1865
                     /locus_tag="OO568_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6643129.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            1862..2572
                     /locus_tag="OO568_004031"
     CDS             1862..2572
                     /locus_tag="OO568_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6643130.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQVGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFV
                     MRTRSKKRRHFQSKVLQK"
     gene            2771..2846
                     /locus_tag="OO568_004032"
     tRNA            2771..2846
                     /locus_tag="OO568_004032"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2804..2806,aa:Ile,seq:cat)
     gene            2848..2924
                     /locus_tag="OO568_004033"
     tRNA            2848..2924
                     /locus_tag="OO568_004033"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2882..2884,aa:Lys,seq:ttt)
BASE COUNT          756 a          700 c          806 g          684 t
ORIGIN      
        1 ggcgggggag aaatccctcg ccaccgctga tgtgtcaggc atcctcaacg cacccgcact
       61 taacccgctt cggcgggttt tgttttttct ggtcgttctg gtttacaatc catccgtcag
      121 cctgaacaac tggcacctgc tgcgccagca gagaaaacag atggcgcacg ataccaaatt
      181 ttacaattcg gataactctg ccgcccctgc cagcaggcac gggcggcgtt ctcatgcatt
      241 caaatctgac tggtatcagc acgacccctg caccgaagaa caggctgaat ggctgattca
      301 gcgctaccgc agacacggat acgagattaa gaaagccctc agtctcgatt atcgtcactg
      361 gataatcttc gtcaggctcc cttattccga acggccaccg cgtccgtcct gcacattcca
      421 gcaacggatc tggaggtaat gtgcgggtat tacttcgacc tgttctggtt ccggaactcg
      481 ggctggtggt ccttaagcca ggccgtgaat ccatgccggt attccacaat acccgggtac
      541 tggtggagcc ggaaccgaaa agcatgcgta atctgccgtc cggggtcgtt cctgccgttc
      601 gccagccgct ggtggaagac aaaacattgc tgccgttttt cagtaacgca caggtgattc
      661 gtgctgctgg tggtgctggt gcattgtctg actggctgtt gcgccatatt aaatcctgcc
      721 agtggccaca cggcgattat catcacagcg aaaccgtcat tcaccgttat ggtaccggcg
      781 caatggtgtt gtgctggcac tgcgacaacc agttgcgtga ccagacatcc gaatcactcg
      841 agcaacttgc tcatcaaaac ctgtcagcat ggatgattga cgtcatcggt cacgcaataa
      901 gcggtacgca ggagcgtgaa ttatctctgg ctgaattatc ctggtgggcg gtccgcaatc
      961 aggtggcgga cgcgctaccg gaagcggtat tacgtcgttc gctggggttg cgtgcggaaa
     1021 aaatccgctc aatgtaccgt gaaagcgaca tcgtgccggg agagcagacc tccaccagca
     1081 tactgaagca gcgcacaaaa aatcttgcgc cgctgcctca cgcccaccag caacagaatc
     1141 caccacagga agagacggtg gtcagcattg ccgttgatcc ggaatctccg gctcagtatc
     1201 tccagcgcca gaaaccacaa cgggaagaga tgcctgtata cacgcgctgg gtaaaaacgc
     1261 agaaatgcat gacgtgtggc aatcaggcag atgatccgca ccacatcatt ggtcatggac
     1321 tgggagggat gggaacaaag gctgacgatt tgtttgttat tccgctgtgt cgtaaatgcc
     1381 ataacgaact acacgccggg gtaaaagatt ttgaagaaaa acacggtagt cagttgttgt
     1441 tgctgattcg ttttttaatg cacgcgagaa attcgggtgt tctgaagtgg aaagcataaa
     1501 tgaccgaacg catagaattt gttttgcctt acccgccaac ggtgaacacc tactggcgac
     1561 gtcgtggcag cacatatttt gtatcaaaag ccggtgagcg ttatcgccgt gatgtggcgc
     1621 taattgttcg ccagcagcga ctgaaattaa acctgtccgg aaggctggcg ataaagatta
     1681 ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaag gcaccgctgg
     1741 atgcgctgac gcatgctgga ctacttatag acgacgagca gtttgatgaa atcaatattg
     1801 tgcgcggtca gctcgttcct ggtgggcggc tggggataaa aatcacagaa ctggggtgcg
     1861 catgaataac cagtatttac agtttgtgcg tgagcagctc attatcgcca ccgctgattt
     1921 gagtggggca acaaaaggtc agcttgaagc ctggcaagag aatgccatgt tcgatacagg
     1981 gcgttacagg cgtaaaaaaa tccggtaccg cgatgaagtg actggaaaaa tgataacgcg
     2041 ggataatcca ccaatcccgg gaaagcaatc gctggcgaag gggacgtcaa ttcctctggt
     2101 cagtccggtt gagttttcga catcatcgtg gcggcgggct gttctgtctc ttgaagaaca
     2161 tcataaagcc tggttgttgt ggtgttacag cgggagtatt tgttgggaat atcagatcgc
     2221 gataacacag tgggcgtgga atgaatttaa tgctcaatcc ggtaccagaa aaattgcagg
     2281 gaaaacgcag gaacgcctga aaaaattaat ctggctggcg gcgcaggcag taaaagcaga
     2341 actttttggt ggggaaggtt atgaatacca ggttggtggg gaaggttatg aatatcagga
     2401 gctggcatta ctggcgggag taacaaccaa aaactggtcc aaaacattta ctggtcactg
     2461 ggttgtaatg aaacacattt ttcaccgact ggatagtgag gctttattgt ttgtaatgag
     2521 aacacgttca aaaaaaagac ggcattttca aagcaaagtg ttgcaaaagt agattgaaag
     2581 gcatatattc atgcaaatct gatattttgc cgattttgta cgtgatggca aaagcaaaca
     2641 aaacccgccc acaagcgggt tttttgtgcc acttatctcg gatagacatg gtgaatgcgc
     2701 tggtggagga gataagggtg atttttgaat gcttgcaaca ttgatttcgt aacgttatta
     2761 tcctgcgctc ggccctttag ctcagtggtg agagcgagcg actcataatc gccaggtcgc
     2821 tggttcaaat ccagcaaggg ccaccaaccg ccactagctc atcaggaaag agcgtcaacc
     2881 ctttaagttg agtgtgcgag gttcgagtcc ccggtggcgg tccagtgccg acttggctca
     2941 gtaggt
//
LOCUS       DAJTYG010000216         2602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTYG010000216 DAJTYG010000000
VERSION     DAJTYG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.218"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..722
                     /gene="yeeE"
                     /locus_tag="OO568_004034"
     CDS             <1..722
                     /gene="yeeE"
                     /locus_tag="OO568_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6643131.1"
                     /translation="FTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLV
                     TVLLVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAW
                     PLSEATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFR
                     VRAADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWT
                     ASWLVYVRPQRKARLATAAAN"
     gene            736..963
                     /locus_tag="OO568_004035"
     CDS             736..963
                     /locus_tag="OO568_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6643132.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1006..2430)
                     /gene="sbcB"
                     /locus_tag="OO568_004036"
     CDS             complement(1006..2430)
                     /gene="sbcB"
                     /locus_tag="OO568_004036"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6643133.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWQEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
BASE COUNT          616 a          631 c          664 g          691 t
ORIGIN      
        1 ttttcactta tatggtgatg agtgcggtga tgcgttctcc acacgccagt ggtttaaatc
       61 aaaccttgca gcactacact actgaacata actctattgc tgagactttt aatttgtctg
      121 tgtggccgtt ggttaccgtt ttgctggtga taacgctctg ggtggtgatg aaagagttga
      181 agaagccaaa actcaaagtc gcgaccttac cgccgcgccg aaccgggatc gctcatattc
      241 tgtttgaaaa acgctggcat ccctttgtca ctgctgtact catcggtttg attgcgcttt
      301 tagcctggcc cctgagtgaa gcaaccggac gcatgtttgg gttaggagtc acttccccaa
      361 cggccaatat tctgcaattt ctggtcgcgg gtgacgtgaa atacattaac tggggcgttt
      421 tcctggtgtt agggatcttc gtgggggcat ttattgcagc caaagcgagc cgtgagttcc
      481 gcgttcgcgc agctgatgca caaacaacat tacgtagcgg gttaggtggt gtactgatgg
      541 gcttcggtgc cagtattgca ggtggttgct ctatcggtaa tggactggtt atgactgcaa
      601 tgatgacctg gcagggctgg attggccttg tatttatgat tctcggagtc tggactgcgt
      661 cctggcttgt gtatgttcga ccgcagcgta aggcgcgact ggcaactgct gcggcaaatt
      721 aagtaaggtg taaagatggc gattaaaaag ctggatgtgg tgacgcaggt ctgtccgttt
      781 ccactcatag aagcaaaagc agcactggca gaaatggcca gcggcgatga gttggtaatt
      841 gagtttgatt gtacccaggc aactgaagca atcccacagt gggcggcaga agaaggacat
      901 gccatcaccg attatcagca gattggcgat gcagcgtgga gcatcaccgt tcaaaaagcc
      961 tgactcaaca ttgtcctccg ccgtaccagc ggcggcggct tcaaattaga cgatctcttc
     1021 cgcgtactgc caaagtgctt ttaacagcgc cactttctct ttgtcatcgg catattgttg
     1081 taccagcatc tgcaattcat cagcataacc ctgcaaaaac tctggcgtga agacctggtg
     1141 gcggtgctcc tgccagcgtt gctgctcggc ataatccagc gtccccggga agttgcgtgc
     1201 ccgataattg aacaacaact tttcaattcg tttatcaaca aaagtgatat ccagtgccgg
     1261 taaattacgc ggctcggttt ccagcacaat tttcattgct gcacgatcgg cgtcactgaa
     1321 aaagccatta taaagctgtg catccacatt atctgaaggt gtaaacggtt cggcttccgc
     1381 gaatatcgct accacttttt cacgcacttg cggattttca cgcagaattt ttaggttatc
     1441 gaggcaatgc tgacgattaa ttcccagtcg gtcggcatct tccgggcgta gcgtattcgc
     1501 ctgggccagt accggacatt tattgatatg caccagctta accggaacgg cggcgttatc
     1561 gccaagatcg gcttttgcgg tatataaacg ctcgcgcaat gtgtcgatat ccagctccag
     1621 taatggcgaa atgtctcctg ccaaatccgc cataattacg gcattgcgat tttcaggatg
     1681 ccacgccagc ggtgccaccc agctggtatt gccacgccat gccccaaaca ttccggaaac
     1741 gtgagccagg ggtttcatct gcggaacatc aatcaacgcc atcagtttgt gtttattacg
     1801 atgggtaaag agataatcaa acaggcgtgg ctgacgcgtt tttaccagtt tcgccatcgc
     1861 aatagtggcg tacacatcag ccatcgcatc gtgggcgttg ctatgttcaa taccattcgc
     1921 tttggttaaa tgctcaaggc gaaagctcgg tagaccgtca tcattttcag gccagtttat
     1981 tccttccggg cgcagggcat aacaggcacg cataacatcc agtaaatccc agcgcgagtt
     2041 atcatgctgc cagctccagg cgtaaggatc gtagaaatta cgataaaaaa cgttgcgtgt
     2101 gacttcgtcg tcgaaacgca cattgttgta gcccagaata caggtcttcg gtacggtaaa
     2161 aagcgagtga atacgggcgg caaacgcggc ttcgttttct cctttcgccc gtgcctcctg
     2221 cggggtaata ccggtaatta atactgctcc aggccggggt aaatagtcat ccgcgggctt
     2281 gcagtaaaag acttcgggtt cgccgatgac attgaattcg tcatcggtgc gaatggctgc
     2341 gaactgtgca gggcgatcta acgcggggtg cgtgccaaag gtttcgtaat cgtgaaagag
     2401 gaaggtaggt tgtttatctg tgtctgtcat tgtctagcat tgtccactgt agttaattaa
     2461 ctgtctgtta taactcgaaa agcaaccctt ttttcacctg agtatgtcta cgaacgtcaa
     2521 atatagttgg atttgcccct atatttccag acatctgtta tcacttaacc cattacaagc
     2581 ccgctgccgc agatattccc gt
//
LOCUS       DAJTYG010000217         2504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTYG010000217 DAJTYG010000000
VERSION     DAJTYG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.219"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            155..1108
                     /locus_tag="OO568_004037"
                     /pseudo
     CDS             155..1108
                     /locus_tag="OO568_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="OO568_004038"
     CDS             complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="OO568_004038"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6643134.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          691 a          615 c          561 g          637 t
ORIGIN      
        1 tattgcgata tggttacatt aaggacaaag catctctaat gactttagct caacgattaa
       61 taacattttt tcattaattt aagttggcta aatgcagtta aaatttttaa cgaccagccc
      121 cccaaaatcg tcgcttccat tttccggagt aataatgaag catgaattat caagtatgaa
      181 ggcatttgtc atactggcag agtccagttc atttaacaat gccgctaaat tactcaatat
      241 tacgcaacct gcattaacgc gaagaataaa aaagatggag gaagatttac atattcaact
      301 atttgagcgt acaactcgca aggttacgtt aacaaaagca ggaaaaaggt tgctcccgga
      361 ggcgcgggaa ttaataaaga aattcgatga aacgcttttt aatattcgtg atatgaatgc
      421 ttatcatcgt ggtatggtga cattagcgtg tattccgacc gcagtatttt attttttacc
      481 gctggcaatc ggtaaattta atgagctgta tcccaatatt aaagtgcgga ttctggaaca
      541 aggcacgaat aattgcatgg aatcggtgct gtgtaacgaa tcagactttg gcataaacat
      601 gaacaacgtc acaaattcat ccattgattt taccccactg gtcaacgagc cattcgtgct
      661 ggcctgtcgg cgtgaccatc cattggccaa aaagtagctt gtagaatggc aggagttagt
      721 gggttacaaa atgattggcg tacgttcctc cagcggcaac cgattgttga tagaacaaca
      781 gctagccgac aagccctgga agctggactg gttttacgaa gtgcgtcatc tttcgacgtc
      841 attaggacta gtggaggcag gactggggat ttcggcgctt cctggtctgg caatgcccca
      901 tgcgccctat tcttcgatta ttggtatccc actggtggaa cccgttatac gtcggacatt
      961 ggggattatt cgccgcaaag atgccgtgct ttctccggca gctgaacgct tttttgcctt
     1021 gctgattaat ttgtggactg acgataaaga caatttgtgg accaatatcg tcgaacgcca
     1081 gagacatgcg ttacaggaga taagttaata caccggcgca ggagagactg cgccggttaa
     1141 atcagcggtt agtgtgcgtt aaccaccacc cacattggtc cctgcccgac cgcatagcgg
     1201 cctttttcat gcagtagccc ctgctcgcca acaatttcgt ataccgagat gtggtgagat
     1261 ttttgcccag cggcaatcag atacttgccg ctgtaatcaa cattgaagcc gcgcggctgg
     1321 gtttccgttg gctggaagcc ttctttactc aacacgctgc catcttccga aacgctgaaa
     1381 acggtaatca gactggcggt acggtcgcag gcgtataaat ggcgaccatc cggggtgata
     1441 tgaatatcag ccgcccaacg ggtgtcggag aagttttccg gcatcatatc cagcgtctgg
     1501 acacattcga tattgccgtg cggatctttc agttcccaga catccactga gctgtttaac
     1561 tcattgacgc aataagcata ttgttcgttt ggatggaata ccatatgacg cgggccggcc
     1621 ccttcaacgg tagtcacttc cgcagggtcc tgcgccacca gatgaccatc atcgctgacc
     1681 gtaaacaggc aaatgcgatc ttgctttaat gccggaaccc acagcgtacg gttgtccggt
     1741 gagatattgg cggaatggca accgtccagc ccctcgacta catcgacgac gcccactggc
     1801 aggccatctt ccagacgcgt tacgctcacg ttacccgcat tgtaagaacc tacaaagaca
     1861 aactgcccct ggtgatcggt ggaaatatgc gtcggactac ccggcagcac agactctgcg
     1921 gcaaaggtca gtgcgccatc gtccggggca atacgatacg ccaggacgcg aaactcaggg
     1981 cgaacaccaa catagagata acgtttgtcc gggctgacca ccatcggctg cacctgcccc
     2041 ggcacatcga caacctgtgt cagcgtcagt gcgccttcat gattcaggtt ccagacgtga
     2101 atttgctggc tctcagggct ggcgatataa actgtttgct tcatgaacac acctcaaaaa
     2161 tgataaatcc cttatctttc atacatataa caacacttgc tggataaaaa acagacaaca
     2221 aaacacgata aaatacaata tcgcacatca attaaatcaa taagttagca ctccatttcg
     2281 aatggcatat tactaaaata ttagtcaccg acacgaattt gacaatgcca cagatatttt
     2341 cgcgactatt cagggagtat gactctcccc gcttttgttt acctgtatca acgaccgtaa
     2401 acactatgcc ataacaactt ctggccaact tcccattcgc tcaacaatca actcacttcc
     2461 ggcacaaata aacgcattac gttgtagatc ggtattctgg tcat
//
LOCUS       DAJTYG010000218         2436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTYG010000218 DAJTYG010000000
VERSION     DAJTYG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.220"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            23..1075
                     /locus_tag="OO568_004039"
     CDS             23..1075
                     /locus_tag="OO568_004039"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR6643135.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNSLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMCLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1151..2436
                     /locus_tag="OO568_004040"
                     /pseudo
     CDS             1151..2436
                     /locus_tag="OO568_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
BASE COUNT          581 a          547 c          625 g          683 t
ORIGIN      
        1 tttttaaatt gagggcatta ttatgaaaaa aataccctgc gtgatgatgc gaggtggaac
       61 ctcgaggggc gcgttcctgt tagcggaaca tttacccgaa gatcaaacgc agcgcgataa
      121 aatattgatg gcaattatgg gttccggtaa cgatctggaa attgacggta ttggcggcgg
      181 taattcactg accagtaaag tcgccattat tagccgttcc agcgatccgc gtgctgatgt
      241 cgattatctg tttgctcaag ttatcgtcca tgagcaacgt gtcgatacca cgcctaactg
      301 cggcaatatg ctgtctggcg taggggcatt cgccattgaa aatggtttga ttgcagcgac
      361 ttcgccagtt acccgtgtac gtatccgcaa cgtcaatacg ggtacgttca tcgaagctga
      421 tgtgcaaacg ccaaatggtg ttgtcgagta cgagggtagc gccagaattg acggcgtacc
      481 gggtactgcc gcaccggttg cgctcacttt cctgaatgcc gctggaacga aaaccggaaa
      541 agttttcccg actgataatc agattgatta ttttgacgat gtcccggtga cctgtatcga
      601 tatggcgatg ccagtcgtca ttattccggc tgaatatctg ggaaaaacag gttatgaatt
      661 accggcggaa ctggatgccg acaaagcatt attagcccgc attgaatcta tccggctaca
      721 agcgggtaaa gcaatgtgct taggtgatgt cagtaatatg gttatcccta aacctgtgct
      781 tatttctcca gcgcagaaag gcggggcaat taatgtgcgt tattttatgc cgcattcttg
      841 ccatcgcgcg ctggcgataa ccggtgctat tgctatttcc agtagttgtg cattggaagg
      901 caccgtcacc cgacaaatcg tcccttctgt aggatatggc aatatcaata ttgaacaccc
      961 cagtggtgcg ctcgacgttc atttaagtaa tgaaggtcag gatgccacga cgttacgcgc
     1021 atctgttatt cggacaacca gaaaaatatt ttccggtgaa gtttatcttc cctgaaaaaa
     1081 ttcgttgtca ggataaggac aatcaataaa ggacttctgt atgagtcata cagaaagaac
     1141 aggattttaa atgaataaga aatcgttatg gaagctaatt ctgatattag cgatcccatg
     1201 tattattggt tttatgccag ctccggcagg attaagcgaa ctggcgtggg tgctttttgg
     1261 tatttacctg gcggccattg tgggtctggt tatcaagcct ttcccggaac ctgtcgtact
     1321 gttaattgcc gttgctgcct caatggtggt ggtcggtaac ttatccgacg gtgcgtttaa
     1381 gaccaccgcc gtattaagcg gttactcttc aggtactacc tggctggtgt tctcggcgtt
     1441 taccttaagc accgcatttg tgaccaccgg tttaggtaaa cgtattgcct atctgctgat
     1501 tggtaaaatc ggtaacacca tgctgggtct gggttacgtt acggtattcc tcgatctggt
     1561 actggctccg gcaacaccgt ctaacaccgc gcgtgcgggc ggcattgtgt taccgatcat
     1621 caacagcgtg gcggtggctt tggggtccga accggaaaaa agtccgcgtc gtgtcggaca
     1681 ttacctgatg atgtccattt acatggtcac caaaaccacc agctatatgt tctttaccgc
     1741 aatggcgggg aacattctgg cgctgaaaat gatcaacgac attctgcacc tgcaaattag
     1801 ctggggtgga tgggcgctgg ccgccggatt gccggattgc cggattgccg ggcatcatta
     1861 tgctgctggt caccccgctg gtaatttaca ccatgtatcc accagaaatt aaaaaggtgg
     1921 ataacaaaac catcgctaaa gcgggccttg ccgaactggg accgatgaaa atccgcgaaa
     1981 aaatgctgct cggtgtcttt gtgctggcgc tactgggctg gattttcagt aagtctctgg
     2041 gggttgatga atccactgtg gcaatcgttg ttatggcgac catgctgctg ctgggtatcg
     2101 ttacctggga agacgtggtt aaaaataaag gcggctggaa taccttaatc tggtacggcg
     2161 gtattatcgg cttaagctcc ttattatcga aagttaaatt tttcgaatgg ttagctgaag
     2221 tctttaaaaa taacctggca tttgatggtc acggtaacgt tgctttcttc gttattattt
     2281 tcctcagcat catcgtgcgt tatttcttcg cttccggtag tgcctatatc gttgctatgt
     2341 tacctgtatt tgccatgctg gcgaacgtct ccggcgcacc gttaatgtta accgcgctgg
     2401 cactgttgtt ctccaactcc tatggcggca tggtta
//
LOCUS       DAJTYG010000219         2405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTYG010000219 DAJTYG010000000
VERSION     DAJTYG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.221"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(339..605)
                     /gene="ospE2"
                     /locus_tag="OO568_004041"
     CDS             complement(339..605)
                     /gene="ospE2"
                     /locus_tag="OO568_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HCR6643136.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(787..1188)
                     /locus_tag="OO568_004042"
                     /pseudo
     CDS             complement(787..1188)
                     /locus_tag="OO568_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1617..2405
                     /gene="virF"
                     /locus_tag="OO568_004043"
     CDS             1617..2405
                     /gene="virF"
                     /locus_tag="OO568_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR6643137.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          741 a          426 c          447 g          791 t
ORIGIN      
        1 aagccgtcta tgggaaggag tctgctgtta ttccagagaa tggtaattca gtcatatatt
       61 tctccatcta tgacttgtca ataccatcct gatatttgtg ggtcagcaat atattctact
      121 aaaaggtatt taaggtcatt acgtccctgc actcgaataa tggtgaagaa agcatgataa
      181 atatatcacg ataacgttca gtatgtatcg aggatagatg gagttactcc tctctgaaat
      241 agaattactg ctctactttt tctgtttcca gaaattatta gtaacaaacc ctgatatatt
      301 attttgaaaa tggagcttaa accagtctga aattaacttc agaaatcaga ataccgttgg
      361 ctttgttttt catcaattcc ggcttcattt atctttgtca accattgcat ttctgtagca
      421 gtgaatttca acgttttcgt ccggttggca gcaggtattt gtaattcgta tatagctgct
      481 tttttattga aaacagtata tccatctgta gtaacagtag aagataataa tacgggattt
      541 gatacttcaa ggatgatatt ttcttgtttt tgtggaaggc aagggaatat agtttgtgta
      601 agcatattgc ttccttatgt tatattttag taaagaattt attttttaca ttatagtcgg
      661 ctaaatgaag tttactgtaa aaaaacgata cttttttcgt aagcgatgag atgtggagcc
      721 agaaaaatgg ctgcgttacg tcattgaact gtccttgacc cacgatattc agacagtcgt
      781 tgtatcttaa gttgacatta cccgctgacg tcgatattcc cttggtgaga gataacccaa
      841 cgcactatgc ggatggtttt cattgtaatg ctcgatcgcc actgcaaggt tatgcaatgc
      901 cgttcttggt ttcggcatga acgcgatgca gtcttccttc atcgttttca cgaaccgttc
      961 tgctattcca ttactctgag ggctactgac tgccgtcgtg cagggctcca gattcaactc
     1021 tctggcgaac tgccgtgttt catgagccgt ataagccgaa ccgttatcgg ccactggatt
     1081 ggccgttccg gtcctttatc gccatatcgc tcctgacatg acatcctgca ccgttgcttt
     1141 gtcataatct cccgtgcctg ctgcccagtc tatggcttca cgatcgcaat atggaacgta
     1201 acgctgtcat cgggagaatc gtcaatgcgg cattcggtta acgcttacca atatcaacac
     1261 caaccggatt tgtggagcct ccagtctgaa ggggctttat gcgttccgta taggatatta
     1321 tgatgctgga gttttgcgaa gctccactcc tttccgaaca tttacacaga tattgcctaa
     1381 gaaaagtgaa accataatat ggttatagtc cctttcagtg caaatactta gcttgttgca
     1441 cagagaaata gaagctgcat aagctctttc ttcaaaaaat gtaaataaag ttaaatatag
     1501 gaaaaattac ttaatctatc ttaataacgg aaagattttg tatacaatca ctgttacaga
     1561 aatttcttag ttactctgta aacactaaat atagtttggt atattctgtt gaatttatga
     1621 tggatatggg acataaaaac aaaatagata taaaggttcg cttgcataac tatattattt
     1681 tatatgcaaa aaggtgttca atgacggtta gctcaggcaa tgaaactttg actatcgatg
     1741 aagggcaaat tgcttttata gagcgaaata tacaaataaa cgtctccata aaaaaatctg
     1801 atagcattaa tccatttgag attataagcc ttgacagaaa tttattatta agcattatta
     1861 gaataatgga accaatttat tcatttcaac actcctattc tgaggagaaa agggggttaa
     1921 acaaaaaaat attcctcctc tctgaggagg aggtttctat cgatttgttc aaatctataa
     1981 aagagatgcc tttcggcaaa agaaagatct atagtttagc ttgcctttta tcagctgttt
     2041 ctgatgagga agctttatat acttcgatat cgatagcttc ttctcttagt ttttctgatc
     2101 agataaggaa gattgttgaa aaaaacatcg agaagagatg gcgtctttct gatatttcaa
     2161 ataacttgaa tttatcagaa atagctgtta gaaaacgatt ggagagtgaa aaattaacat
     2221 ttcaacaaat ccttcttgat attcgcatgc atcatgcagc aaagctttta ttgaatagtc
     2281 aaagctatat taatgatgta tcaagactta tcggaatatc aagcccatct tattttataa
     2341 ggaaatttaa tgaatattat ggtataactc caaagaaatt ttacttatat cataaaaaat
     2401 tttaa
//
LOCUS       DAJTYG010000220         2382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTYG010000220 DAJTYG010000000
VERSION     DAJTYG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.222"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..108
                     /locus_tag="OO568_004044"
     CDS             <1..108
                     /locus_tag="OO568_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HCR6643138.1"
                     /translation="AVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            132..2382
                     /locus_tag="OO568_004045"
                     /pseudo
     CDS             132..2382
                     /locus_tag="OO568_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
BASE COUNT          562 a          617 c          640 g          563 t
ORIGIN      
        1 gcagtcagtc tgcaacaact tatgacatgg aaggacaccc gatggatacc caacgattcc
       61 aatcccaatt tcattggcat ttatcgttta aattttctgg cgcgatagca gcgtgcttgt
      121 ccctgtctct tgtgggaaca gggcttgcta aagctgatga ttcgcttcct tccagtaact
      181 atgcgccgcc cgccggggga acattctttt tgcttgctga cagcagtttt agcagcagtg
      241 aagaggcgaa agtgcgactg gaagcgccgg ggcgtgatta tcggcgctat cagatggaag
      301 agtacggcgg cgtggacgtt cgcctgtatc gtattcctga cccgatggca tttttgcgcc
      361 agcagaaaaa cctgcatcgc attgtggtgc aaccgcaata tctgggcgac gggctgaaca
      421 atacgctgac ctagctgtgg gataactggt acggcaaatc tcgccgcgtg atgcagcgta
      481 ctttctcttc tcagtcacgg cagaatgtga ctcaggcatt acccgaatta catctcggca
      541 atgccattat taaaccttcc cgttatgtac agaacaacca gttttccccg ctgaaaaaat
      601 atcccttggt ggaacagttc cgttatccac tatggcaggc taaaccggtc gagccgcagc
      661 aagggataaa actggaaggc gcatccagca atttcatctc gccgcagccg ggtaatattt
      721 atattcctct cggccaacaa gagccggggc tgtacctcgt cgaggcgatg gtaggtgggt
      781 atcgggcgac gacggtggtg tttgtttccg ataccgtggc gcttagcaaa gtgtcaggta
      841 acgagcttct ggtatggacc gcgggtaaaa aacagggtga agcgaagccc ggctcagaga
      901 tcttgtggac tgacggtctt ggcgtgatga cccgcggggt gaccgatgac agcggtactt
      961 tgcagttaca acatatatcg ccagaacgtt catacattct gggtaaggat gctgaaggcg
     1021 gcgtttttgt ctccgagaac ttcttctacg aaagcgaaat ctacaacacc cgcttgtata
     1081 tttttaccga tcgcccgcta tatcgcgcag gcgatcgtgt cgatgttaaa gtgatgggcc
     1141 gcgagttccg cgatccgttg cattcatccc ccatcgtcag cgccccggcg aagctttcgg
     1201 tgctggacgc taacggcagt ctgttgcaaa ccgtcgatgt cacgctggat gcgcgcaatg
     1261 gcgggcaggg aagtttccgc ctgccagaaa atgccgttgc cggaggttat gagttacgtc
     1321 ttgcttaccg caatcaggtc tatagcagca gttttcgcgt ggcaaactgc atcaagccac
     1381 atttcgagat tggtttagct ctcgacaaaa aagagttcaa aactggcgaa gcggtcagcg
     1441 gcaaactgca actgctctac ccggatggcg agccggtaaa aaatgcccgc gtgcagttaa
     1501 gtttgcgcgc tcagcaatta tcaatggtcg gtaacgattt gcgttatgcc ggacgtttcc
     1561 ccgtgtcgct ggaaggcagc gaaacggtgt ccgacgccag cggtcatgtg gcgttaaatc
     1621 tccccgccgc cgataaaccg agccgctatt tgttaaccgt ctccgccagt gacggcgcgg
     1681 cgtatcgcgt caccaccacc aaagagatcc tcattgaacg cggcctggcg cattactctt
     1741 taagtaccgc cgcacaatac agtaatagcg gcgagtcggt tgtgttccgt tatgccgcgc
     1801 tggaatcttc aaaacaggtt cctgttacgt atgaatggtt gcgtctcgaa gaccgcacga
     1861 gccatagcgg agagctaccg tcaggcggca aatcctttac cgtcaatttc gctaaacctg
     1921 gcaactacaa tctgacatta cgcgataaag acggcttaat tctcgctggg ttaagtcatg
     1981 ccgtcagcgg taagggcagc acggcgcata ctggtacggt agatatcgtg gcggataaaa
     2041 cgctgtacca gccaggcgaa accgcgaaga tgctgattac tttcccggag ccaattgatg
     2101 aagcgttatt gacgctggaa cgcgatcgcg tggaacagca gtcgctgctt tcgcatccgg
     2161 caaactggct aacgctacaa cgtttaaacg ataccctgta tgaagcccgg gttccagtga
     2221 gcaattcctt tgcgcctaac atcacttttt cggtgctgta tacccgtaat ggtcagtaca
     2281 gttttcagaa cgccgggatc aaagttgccg ttcctcagct tgatatccgg gtgaaaacgg
     2341 acaaaaccca ttaccagcct ggtgaactgg tcaatgtcga at
//
LOCUS       DAJTYG010000221         2356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTYG010000221 DAJTYG010000000
VERSION     DAJTYG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.223"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..94)
                     /locus_tag="OO568_004046"
     CDS             complement(<1..94)
                     /locus_tag="OO568_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR6643139.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGN"
     gene            complement(135..590)
                     /gene="csgB"
                     /locus_tag="OO568_004047"
     CDS             complement(135..590)
                     /gene="csgB"
                     /locus_tag="OO568_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6643140.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1344..1994
                     /gene="csgD"
                     /locus_tag="OO568_004048"
     CDS             1344..1994
                     /gene="csgD"
                     /locus_tag="OO568_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6643141.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            1999..>2356
                     /gene="csgE"
                     /locus_tag="OO568_004049"
     CDS             1999..>2356
                     /gene="csgE"
                     /locus_tag="OO568_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6643142.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALL"
BASE COUNT          692 a          492 c          443 g          729 t
ORIGIN      
        1 ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg gagaatacga
       61 ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg attgatttaa
      121 aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg tctctgcact
      181 acaattgccg ttttttgagt accatactgt gtaatatttg ctttattacc agaacctttc
      241 tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc gttggcactg
      301 cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt tgcccgattg
      361 ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg taactgagca
      421 ctattattag tcccagcttg accaattatg gctgcctgat taaatgaaga cttactcaat
      481 tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc ggctgcaatc
      541 ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat gttgtcaccc
      601 tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta acgcaacctg
      661 tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt ttaataactc
      721 acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc cgcgtaaata
      781 cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt ccgctatcaa
      841 aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg tcttgcgcac
      901 aagccgtgac aaacctcatc tttttgcagt aaaaaaattg tccacggagg tgtggagaaa
      961 aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tattttttat atgtattatt
     1021 agtaagttag caccatttgt atgatttttt aaaattgtgc aataaaaacc aaatgtacaa
     1081 cttttctatc atttctaaac ttaataaaac cttaatgtta acattttaat ataatgagtt
     1141 acattttgtt acatgtttaa cacttgattt aagatttgta atgcctagat tgaaatcaga
     1201 tgtaatccat tagttttata ttttacccat ttagggctga tttattacta cacacagcag
     1261 tgcaacatct gtcagtactt ctggtgcttc tattttagag gcagctgtca ggtgtgcgat
     1321 caataaaaaa gcggggtttc atcatgttta atgaagtcca tagtattcat ggtcatacat
     1381 tattgttgat cactaaacct tctttgcagg cgacagctct cttgcagcac cttaaacaat
     1441 cgctagcaat tacaggaaaa ttacataata ttcaacgttc tctggacgat atctcttcag
     1501 gctctattat tcttctggat atgatggaag cggataaaaa gcttatccat tattggcagg
     1561 atactttgag caggaaaaac aacaatatca aaatattgtt gctaaatacg cctgaagatt
     1621 acccgtaccg cgacattgaa aactggcctc atatcaacgg cgttttttat gccatggagg
     1681 atcaagaacg tgttgtcaat gggttgcaag gcgtcctgcg tggcgaatgc tactttacgc
     1741 aaaagcttgc cagttacctg attacacatt ctggtaacta tcgttataac agcacggaat
     1801 cagccctcct tactcatcgg gaaaaagaga tcctgaataa gctgcgtatc ggcgcgtcta
     1861 ataacgagat cgctcgttcg ttgttcatca gcgaaaatac ggttaaaacg catctttata
     1921 atcttttcaa gaagatagcc gtaaaaaacc ggacacaagc ggtttcctgg gcaaacgata
     1981 acctcaggcg ataaagccat gaaacgttat ttacgctgga ttgtggcggc agaatttctg
     2041 ttcgccgcag ggaatcttca cgccgttgag gtagaagtcc cgggattgtt aactgaccat
     2101 actgtttcat ctattggcca tgatttttac cgagccttta gtgataaatg ggaaagtgac
     2161 tatacgggta acttaacgat taatgaaagg cccagtgcac gatggggaag ctggatcaca
     2221 ataacggtca atcaggatgt tattttccag acttttttat tcccgttgaa aagagacttc
     2281 gagaaaactg tcgtctttgc actgattcaa actgaagaag cactaaatcg tcgccagata
     2341 aatcaggcgt tattaa
//
LOCUS       DAJTYG010000222         2343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTYG010000222 DAJTYG010000000
VERSION     DAJTYG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.224"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            269..406
                     /locus_tag="OO568_004050"
     CDS             269..406
                     /locus_tag="OO568_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase mbeA domain protein"
                     /protein_id="HCR6643143.1"
                     /translation="MEKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVISFQGKEWVHLK
                     E"
     gene            413..589
                     /locus_tag="OO568_004051"
     CDS             413..589
                     /locus_tag="OO568_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6643144.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQV"
     gene            complement(948..1415)
                     /locus_tag="OO568_004052"
     CDS             complement(948..1415)
                     /locus_tag="OO568_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643145.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQQLQIQEVCNSTLWETAKQQLLQESDGRYFAINSKHL
                     DFPGSGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFSHNLT"
BASE COUNT          585 a          493 c          665 g          600 t
ORIGIN      
        1 caaggctgtg acatcaaacg agacgaaaat caaagacgct atagccctgt tcacagcttc
       61 gacagaggaa tcactgaaaa aacaccggga aggggtgaaa gaggcgatga tgcagcacag
      121 gaaggacgtg ttaaagctgg cagggaatac gggcatgatg ttactgggga tggtcatttt
      181 cctgtttacc gtgagtggcg ggacgctctg gtatcttggc gggatgatac aggcgaacct
      241 ggaagaaatc aggaagcaga acgagagtct ggagaagctg aacgcgaaga catggggcgt
      301 ggagtttgtg caggacggga acaggaaatt tctggtcctt ccttacggga agtcagcaga
      361 ggtgatttcc tttcagggga aggaatgggt acatctgaag gagtgacaca gagtgacaga
      421 gctggaaaca catttgctga acgccttaga gcagctgcaa caggactata tgcagcggct
      481 gagcgaatgg gagagcgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga
      541 caacgcgatg ctgaacgagc gggtcatgca gttgagtcag caggtgtagc acttgagcga
      601 gcagacagaa cgcttgagcc agttatacag cgagaactgg agataagaga ggaacggctg
      661 atacaggagc gcgaacatgc gttatccctg gaacgggagc gtcagccgga aatacaggaa
      721 cgcacgctgg atggtccttc gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg
      781 attaggaggg gttaatgtgg cctgtaccat ctggttgcat aggtattcat gcggttaaaa
      841 tttatcaggc gcgatcgcgg cagtttttcg ggtggtttgt tgtcattttt atctgtctgc
      901 tgccgtgatc gcaccaaacg cgtttcagtg gtgcgggcaa taagatatta cgttaaattg
      961 tgagagaatg gtccccatat actgacttta tcatacggat agggttggta gtgggtcgat
     1021 tgggtttcgg cattgtttcc gttaatgcca attgcatttt tgtatttgat gcaacagaag
     1081 gcgtgagatt ctcctgagcc tggaaagtct aagtgtttag aattgatggc aaaatatctt
     1141 ccgtccgatt cttgaagtag ttgttgtttg gctgtctccc aaagggtgct gttacaaact
     1201 tcttgaattt gtagttgttg caggactctt attactgcgt tgtcatgttc caattcttta
     1261 ggggagtgta ttattctctc ttttaataag ctgtttacca caggatttag ttgttgtcta
     1321 aagcacgcag ataatgccat tgctacacaa ttttgttccc gaggaagcat gtttggcggg
     1381 gtatgccgtg tttgatgtga tatgtctatt ctcatagaag gtctctggct tatagcatca
     1441 acaaatacgg ctataaaaca aaattttcat agagtgattc aagatcttct tgagatcttt
     1501 tttggcggtg cgtaatctgc tgcctgcaaa caaaaaaacc accgttatca acggtggttt
     1561 gtttgccgga atctagagct accaactctt tcttccgaag gtaactggct tggtggagca
     1621 cagataccaa atgctgtcct tctagtgtag ccgcagttag gccaccactt caagaactct
     1681 taatatctca atccacctta tccagttacc aatggatgct gccagtggcg ttaagtcgtg
     1741 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1801 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacca
     1861 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1921 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1981 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2041 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcggc gcagcctttc tttgcccttt
     2101 gcctttttgc tcacatgttc tttccggcct tatcccctga ttctgtggat agccgtatta
     2161 ccgtctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgt agcgagtcag
     2221 tgagcgagga agcggaagag cgccggaagc tgcattttct ccttacgcat ctgtgcggca
     2281 tttcacatcg gctatggtgc acttttcata caatccgcac tgatgccgca taagtaagcc
     2341 tgt
//
LOCUS       DAJTYG010000223         2313 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTYG010000223 DAJTYG010000000
VERSION     DAJTYG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.225"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..94
                     /locus_tag="OO568_004053"
     CDS             <1..94
                     /locus_tag="OO568_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HCR6643146.1"
                     /translation="WAMMPPSNSLQLLDETFSVSGTESSATRQH"
     gene            complement(227..739)
                     /locus_tag="OO568_004054"
     CDS             complement(227..739)
                     /locus_tag="OO568_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR6643147.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            934..1698
                     /locus_tag="OO568_004055"
     CDS             934..1698
                     /locus_tag="OO568_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643148.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGECDLNDFYSDIQTDAESGFT"
     gene            1649..>2313
                     /locus_tag="OO568_004056"
     CDS             1649..>2313
                     /locus_tag="OO568_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR6643149.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          657 a          508 c          586 g          562 t
ORIGIN      
        1 atgggcaatg atgccaccgt cgaactctct ccagttgctg gacgaaacgt tctcggtatc
       61 cgggacggaa tcatcagcga ccagacagca ttgagaatgc ttcaggagta catcaggatc
      121 caatgccttg gggggtagcg gtaattttac tcattatcct tcaaatcaga ttctgttgtc
      181 agaggaatgg gggaagctgg attcgcaaga gctttttatg ggcagtttag ctaaaaaaag
      241 gcaacacgaa gctaaaacta ccaacataaa gtgctgacca gctagcagct gaaatcagac
      301 tcactgtata cactttcact ggatgaagtt gcaacattcc tgccaccaaa ggagcaatgt
      361 aacgcagcac ggcgataaag cgcgaagtga aaagtattga cacagagtta ttttgcaact
      421 gaaggcgaac ccgttctatt gtattggaac ggctcgatat tatacatgca agttgaggga
      481 tatgcccaat tattatacca agatggtaat taacgatagt accgcaccat gcgccactca
      541 tgaccgtgat tccagcttcc catggtgaaa gagtcgtctt gctgactgta attacagtgg
      601 ccatcataat tgatgctggt ggcaatatcg ccgaaatgag taaagtggat tttgtgaacg
      661 ctattataaa aagaagcccc cataggcgat atggatgaag tacaaaatag ttcatcaaag
      721 catttatcca ctccatcaat gtctttcctt ctgtgaattt ttctttgcta tttaaccgtg
      781 atacgactta atttaggctt aaacacctta catttacaag gtactcctgt ggggggccct
      841 gccacggagc gtcgcaggag tgagtgatga ggaagaatta aagcaaccta ttgtgacagg
      901 caacgataac agtaaatcac aggaagcata atcatggcaa aaccggactg ggaggccatc
      961 gaatcggcat accgggccgg agtccttagc ctccgtgata taggcgagaa atacggcgtt
     1021 acagaagggg ctatcaggaa gagggccaaa aagcttggtt gggcacgcag tggcggtacg
     1081 caggtttgca aaaatggtac gcaaaaaagg aaagtgcgta ccagcagaaa gcctgccatt
     1141 actggcctta cacaaaaaag tacgcaacta aaaacagaat ctacaccgga tacgaaaccg
     1201 atacgcggaa tgcgtaccga tcccccgact aacccattcc aacccggtaa ccagcaggca
     1261 ttaaaacacg gtggttatgc ccgtcgcctt ctgctcaaag atgaggtgat agaggacgct
     1321 aaagcgttga cgctcgagga cgaattattt cgccttcgtg ctaacaacct tgtcgctgca
     1381 gagaatattg gtcggtggtt ggtgtcgctg gaagatgcta atggggacca ggaaaggaag
     1441 atgctgattg aaaatatcag cgccgccgag aaagcaatga tgcgcaatac agttcgtatt
     1501 gagtccatcg ttggcacgct tgcgacggta ggaaaaatat ttgctgatac agcctaccgc
     1561 aaggccgcca ctgataaggt gtctctggag gctgatcgtc ttcgccgtga tgcaggtatt
     1621 gatgatggca atggagagtg tgacctcaat gacttctact ctgacatcca aaccgacgct
     1681 gaatccggct ttacgtagtt tctggactat gcgggcacgt aacaaagtgc tttatggtgg
     1741 tcggtcatcg tcaaaatcat gggatgccgc tggcattgcc atatttctgt cgaataaata
     1801 caccctgcgt ttttgttgtg cccgtcagat ccagaataaa atcgaagagt cggtgtatac
     1861 cctgctcaaa attcagatag acaggtttgg tctgcggcac cgtttccgta ttctgaacaa
     1921 caaaatcatt aaccgggtta ctggctcgga atttgttttt tatggattat ggcgcaacat
     1981 cgaagaaatt aagtcactgg aggggatcga tgtgttgtgg ctggaagaag cccacgcact
     2041 gacggaatac cagtggaaaa ttctggagcc aacgatccgt aaagagggtt cggaatgctg
     2101 gttcatattc aaccccggac ttgttactga tttcgtctgg cgcaacttcg ttgttgatcc
     2161 gcccgaaggc actctcatcc gcaaaattaa ctatgacgaa aatccgtttc tgtctgacac
     2221 catgcttaag gttatcgacg cggcgcgacg ccgtgatccg gatggtttta aacatgtgta
     2281 tgagggcgtt ccggagtctg atgatgatgc ggc
//
LOCUS       DAJTYG010000224         2301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTYG010000224 DAJTYG010000000
VERSION     DAJTYG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.226"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..784)
                     /locus_tag="OO568_004057"
     CDS             complement(<1..784)
                     /locus_tag="OO568_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR6643150.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMS"
     gene            1132..2004
                     /gene="rluF"
                     /locus_tag="OO568_004058"
     CDS             1132..2004
                     /gene="rluF"
                     /locus_tag="OO568_004058"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6643151.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(2137..2301)
                     /gene="yjbD"
                     /locus_tag="OO568_004059"
                     /pseudo
     CDS             complement(2137..2301)
                     /gene="yjbD"
                     /locus_tag="OO568_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
BASE COUNT          562 a          589 c          576 g          574 t
ORIGIN      
        1 cgctcatatt cgtttcaacc attgcgccgt gaatatcaaa aaagcgcgag caaatatcgc
       61 cagcaacctg gcgggcattc aggtcgtcac tctcttcacc accataaaac gcatgccagt
      121 tagcgccgtc gcgaatggcc ggtgagccaa ttcccaccag ggcgacatcc agattatccc
      181 agtaggcaga gatggtttta aagtgctgag actgcatgat cccattacga attaatgggt
      241 tatccagcag agccggaaaa tccgcgagat gcgattcccc tttcagcttc gctgccgcgc
      301 tgtaggttaa tgtgtttaca tgatagcggc tttcgagttt accggacggt ccgccaataa
      361 ttggtacgca gattaactgc cgcgattgcc ccgcctgcgg caagttttca accagtgcgc
      421 tcaccgcgcg accccaggaa aaaccgacaa tatcgccagg ctccagcaag cgatccagca
      481 gttgcgcgcc gtgtaacccc atcatcgcca gttgagtctc ttcatcctca tcatttcccg
      541 acaccaccac aacgtctttc aggccaaact tttgcttcag ttgctgctcc agccagagat
      601 tttcgttgta gtcatagttg atggcgatgg tgaccattcc ctgatcgcgg cctcgtttaa
      661 gcaagcggct gatggtggtg cggtaaatac ccagttcgcg cgcgatttgc gcctgcgtca
      721 tatcctgttc gtaatagagt tgggcaatct tcacaatcaa acggatatcg tcactgtttt
      781 ccattgccgc ctcctggttc tgcacatttg tgcaaatacg ctttctcacc gcttgatctg
      841 atataacccc atcatgaggg cctttcaaag cgatttttca ctctttgcat ggggagaaat
      901 agcgctttct cttgcacatt tgtttctctg cgtagtagtc atcagcacag gtgtgcagcg
      961 attcgtaata tgttgattat ccttattttg ccaggcaaat ttgcatcgta cttccggaga
     1021 cacttttgtg ataaaaggcg tggcagatca catttaattt cgacgttgcc gcataaccct
     1081 gtttttcccc gctaagaggt acggtatatt ccgcgtttta caggagaatt tatgctgccc
     1141 gactcatcag tccgtttaaa taaatacatc agcgaaagcg gaatttgctc acgccgcgaa
     1201 gcggatcgct atatcgagca aggcaatgtg ttccttaatg gcaagcgagc caccattggc
     1261 gatcaggtga aacccggcga cattgtgaaa gtaaacggtc agttgattga gcctcgggaa
     1321 gccgaagatt tggtacttat cgccctgaac aagcccgtcg gtattgtcag caccaccgaa
     1381 gatggcgagc gcgataacat tgtcgatttc gttaaccaca gcaaacgcgt gttcccgatt
     1441 ggccgcctgg ataaagactc ccaggggctg attttcctca ccaatcacgg cgatctggtg
     1501 aataagatcc tccgtgcggg taacgatcat gagaaagagt atctggtgac ggtcgataaa
     1561 ccgattaccg atgaatttat tcgtggtatg ggggcggggg tgccgatcct cgggacggtg
     1621 acgaaaaagt gcaaagtcaa aaaagaagcg ccgtttgtct tccgcattac cctagtgcag
     1681 gggctgaacc gtcagatccg gcgcatgtgc gagcatttcg gctatgaagt gaaaaagctg
     1741 gaacgtacgc gcattatgaa cgtcagctta agcggcattc cgctggggga gtggcgcgat
     1801 ttaaccgacg atgagttaat cgacctcttt aagctcatcg aaaattcctc ttccgaggca
     1861 aaacctaaag cgaaggccaa accgaaaaca gcgggcatca aacgtccagt cgttaagatg
     1921 gaaaaaacgg cggaaaaagg cggtcgcccg gcgtccaacg gtaagcgttt tacctcgccg
     1981 gggcgtaaaa agaagggacg ctgattaacg cctcagactg atgaaaacgt ccaaaaagag
     2041 ctcggacagt cccctcgccc cttcggggag agggttaggg tgaggggaag attgtgtcca
     2101 cacagacgag tgaaaatctc aggcgttgat tgttcattag cgcctgccgc gcgtcgacgt
     2161 atttgctgac cagctaaatg acgcttcagg atccggttta taagcctgct ttttcttcaa
     2221 ttggcgggct tttttcgctt cagcctcacg ctcgaccatt aatttatcga tgtactctct
     2281 ttcttaatgc tgttggtttc g
//
LOCUS       DAJTYG010000225         2300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTYG010000225 DAJTYG010000000
VERSION     DAJTYG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.227"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..156
                     /locus_tag="OO568_004060"
     CDS             <1..156
                     /locus_tag="OO568_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6643152.1"
                     /translation="SLSHYRFKSRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFRI
                     KYYQMTA"
     gene            200..1000
                     /locus_tag="OO568_004061"
                     /pseudo
     CDS             200..1000
                     /locus_tag="OO568_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1115..1456
                     /locus_tag="OO568_004062"
                     /pseudo
     CDS             1115..1456
                     /locus_tag="OO568_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1439..2276
                     /locus_tag="OO568_004063"
                     /pseudo
     CDS             1439..2276
                     /locus_tag="OO568_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          573 a          554 c          685 g          488 t
ORIGIN      
        1 agcctgagcc actatcgttt taagagccgg gatgaggcca tctcagtaat acgggaatac
       61 attgagattt tctacaatcg tcagcgtcgt cactctcgtc tggggaatat ctccccggca
      121 gccttcagga taaaatatta tcagatgact gcttagaaaa agaacaaatg gtggtgtccg
      181 ctattgccag tacacctcag ttctgggcct tgcccgtagc agttattttt atcaccgggc
      241 tgcactgaaa gccggtgata aatacgccac gatacgtaag atgctgacag atatctttaa
      301 cagtaattac cggtgttatg gctatcgtcg cctgcatgcg atgctcaggc atgagggggg
      361 gcggctatca gaaaaggttg tacgcagact tatggtggag gaacagcttg tcgtcagtcg
      421 taaccgtcgt cgtcgctaca gctcatattg cggagaaatc ggaccggctc cggataacct
      481 tatcgccaga gattttaagg cggagcaacc taatcagaaa tagctgacag atatcacgga
      541 gttccagctc cctgcaggta aagtctggct gtcaccagtg gtggactgct tcgatggaaa
      601 agttgtgagc tggtctctca gtacacgccc tgatgctgaa ctggtcaaca ctatgctgga
      661 tagcgctgtc gaaacgttaa atgctggcga acgaccggtg atacacagtg acagaggtgg
      721 gcattatcgc tggccaggct ggctggaaag agtgaatgca gcaggtctta ttcgctcaat
      781 gtcccgtaaa ggatgttcac ctgataatgc tgcatgcgaa ggctttttcg gcagactgaa
      841 aacggaaatg tattatgggc gtaaatggtc gggcatcacg ccagaaaagt tcatgcagca
      901 agtggatgct tacatcagat ggtataacga gcggcgtata aaattatcgc tgggtgccgt
      961 cagccccaaa atgtaccgcc aacaatgcgg gctggaatga taaagcagtc caggaaatcg
     1021 tccgcatccc cccttcacca tttggattgt ccgctccacc caacatgttg tttccttaag
     1081 gttcctacac cacaaaggaa actctcatgc tgcgcagtgg atggctgacg ttgccgacag
     1141 gcgggaactt cgccagttca ggcagacacc ggaacagcgc ttcacgcagg aacaggagca
     1201 tctgcagccg ctgctgggta cggacttcga tatccgtcat gtctcctggg atggctatat
     1261 cgaggttggc gggaaccgtt acagtgtgcc ggaaagcctg tgtggtcagc tggtatcaat
     1321 acggatctcg ctggatgatg aactgcggat ctacagtaat gagcaacagg tagcatcgca
     1381 ccgtctctgt tcggctgcat acggctggca gaccgtgccg gagcatcacg ctccactctg
     1441 gcagcaggct tcggagcgca tggccgaacg gctgggggag atacagaaac gggtaatcac
     1501 tgtctgtgac cgggaagcgg atatctggca ttacctgtat tataaagtca gccacggaca
     1561 gcgtggtgcg tgctgcacag aatcgccggc tggaagaggc acccggcaag ctcttcgaac
     1621 tgccggaagt cctggcaacc gcaggaagcc acacgctgaa tgtgatgcaa aaaggcgggc
     1681 gggcagcccg tcaggcccgg atgttcatca gttacagcga agtcagcata aaaaatcccg
     1741 acaacagcgg ccaggcgctc ccgctcacgt atgtctgttg ccgggagcag gcagaggacg
     1801 gtgcctgctg gcatctgctg acgtcaggaa aagcggcgag tgccgccgat gcacgacgta
     1861 ttgtcagcca ttacgagcga cgctggctga cagaggaata ccacaaggcg tggaaaagtg
     1921 gtggtacatg gaatcgctgc gaatgcagac ccgggataac cttgagcgca tggtggttat
     1981 ccaggcgttt atagcggtac gggtgctggg cctgcggcag ggaggtgtca gtgaagaaac
     2041 acaaaatgac agctgtgaga aaatactgac gccgacagag tggaaactac tgtgggttaa
     2101 gctggaagga aagccgcttc cggtgcaggc accgaccctg aaatgggcct ggggcgatgg
     2161 catgacagca aacgcacagg tcgtcccggt tggggcgtca tgtgggatgg ctggtcagac
     2221 ttcaggatat ggttgagggt tacctggtga tgaagtctct tgatcaggag ctctgatcaa
     2281 gagacagcag cttagggggg
//
LOCUS       DAJTYG010000226         2261 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTYG010000226 DAJTYG010000000
VERSION     DAJTYG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.228"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(87..>2261)
                     /locus_tag="OO568_004064"
     CDS             complement(87..>2261)
                     /locus_tag="OO568_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HCR6643153.1"
                     /translation="FEYHNLDRFEEIFISMDVDDVGREAAREIASRLGEHRCRLVTLP
                     YKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPW
                     ESLADKFQFREAELTLVNGVNGHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGN
                     PSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRL
                     SETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKFSNVEPYMIERMK
                     AELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRVFFSANKSTR
                     IGMLCGLLETDGYVEKDGTLVFSSASEELRNGVVQLVNSLGGSCRTRVKTGVTYTYKD
                     DKRHGMDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRNVEKIGNAECLCI
                     MVDHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLELKPGILLKRLTRQAT
                     CCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRYGIQLFIIDSLMK
                     CGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTGKMDVKGSGAITD
                     LTDNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEKQRNGEGWEGGVP
                     LFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT          528 a          627 c          486 g          620 t
ORIGIN      
        1 gctatcgttc acttccctct cccccaaata aaaaggcctg cgattaccag caggcctgct
       61 attagctcag tgatgtagat ggtcatttaa tactccgtca cgttttcctg tcgccacgcc
      121 tcgtcatatt ccgatttcgg catattggcg atgtagctat atggcgatcc tgattcaagt
      181 tgcaggaact ggtgcgattg ctcgtcaagg aacaacggga caccaccttc ccaaccttcg
      241 ccgttacgtt gtttttcaag catcaaaaca gatgccggag atgccagtag ctgttcgtcc
      301 ttctctgaca tcttttcacc actctgaact ctctgtaacg ctctctcgcg agccttgtta
      361 cgccagatga tgaaaaggtt gtctgtcagg tctgttatcg ctccagagcc ttttacgtcc
      421 attttcccgg ttggtttttc ttcgctgtct ccttttcgcg agtgagtaac gagaatgacg
      481 tgggagtttg ttttgttttt gaagtcgcaa atcgagtcaa caaacgcctt ctgcccgtta
      541 tagtcatcgt cgcctatgcc acatttcatc aggctgtcga tgatgaataa ctggatcccg
      601 tatcggcggc gagcgtagtc gaatatttcg atcagcctgt cggctttcgc cgttccagtc
      661 aggccaaaca cccaaagtct ttcgtcataa aatttaaatg cagagtcaat ttccagcact
      721 ggcggcatct tgcagcacgt tgcctgacgg gtaagtcgct taaggagaat accaggcttc
      781 agctcaagtg acgcgatgca cgtcttcaca ccctgacgca ttgcctcaag tgccatatgc
      841 ccgacaacct cggtgttatg cgtcgccaca tatcccctgg ttacatacag gtggcgaggg
      901 tgatcgacca taatacacaa gcattctgca ttgccgattt tttcaacatt cctgacgaat
      961 accccacagc cctcgaatcg atgcgcagtt aatctgccat tgagtcgtgg tgaacggatg
     1021 gcctccctga tttctcttgt cagtctgatt cttgcctcgt atgaatccat cccatgccgc
     1081 ttatcgtcct tgtatgtgta tgtcacgcca gttttaactc gcgtccggca tgacccgccg
     1141 agtgagttaa ccagttgaac aaccccattg cgcagttctt cacttgctga ggaaaaaaca
     1201 agcgttccat ccttctcgac atacccatcc gtttcaagca gaccacacag catgcctata
     1261 cgcgttgatt tatttgcgga aaaaaacaca cgagggatga atttgttttt tgctgtgcac
     1321 cccattagtc cataacctcg cagggtctcc atgagtggat ttacctgacc acgcgccgtt
     1381 gatatcagcc agtccttacc atctccagag aagttgtaat caggcagttc agccttcata
     1441 cgctcgatca tgtatggctc gacgtttgaa aacttcacgc tcccattgct aagactacca
     1501 tccccgagaa gggagccgat aacccatgct aatggctctg agtggtcgcc aaagtcacca
     1561 gttatttcag gaatcctaac gccatttttg tgcctcttcg tttcactcaa ccgcttcaac
     1621 ccgaaggtat ctatcacgcg gcgtttctcg cctttcgtga atccacgact agtgacctcc
     1681 cataggtgat cgcctgcgca atcaacataa cgaccatctt caaatgtgac tcggtaaaca
     1741 tctctaacac cctgcgggaa tatcccagtg acagttgacg gattgccgtc tactgacgcc
     1801 acctgatcgc caatttttac attcccgtga gtagtccatg tcccatcagc taaaagaata
     1861 ggctcattca gtgggcatgc ctttccgtga ccgttcacac cattgaccag cgtcaactct
     1921 gcctcacgga actggaattt atctgccaga gattcccacg gtggattaaa cagatactgc
     1981 tgcttgccgt agaaagcgtt gatagtgtcc tggtaaaact ctcgcgcact gtagagttct
     2041 tcaggatcga agtaggatgc cgtgccgatg tactgccaga tttcatcctc ggtaacaccg
     2101 ttcatcaggc attcgttgat gtctttgtac ggcagagtaa caagacggca acgatgttca
     2161 ccgagtcggc ttgcgatttc ccttgcggct tcacgaccaa catcatcaac gtccatcgag
     2221 atgaatattt cctcaaacct gtcgaggtta tgatactcaa a
//
LOCUS       DAJTYG010000227         2222 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTYG010000227 DAJTYG010000000
VERSION     DAJTYG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.229"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..672)
                     /locus_tag="OO568_004065"
     CDS             complement(<1..672)
                     /locus_tag="OO568_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6643154.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNG"
     gene            complement(1273..1521)
                     /locus_tag="OO568_004066"
     CDS             complement(1273..1521)
                     /locus_tag="OO568_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6643155.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1876..>2222)
                     /locus_tag="OO568_004067"
     CDS             complement(1876..>2222)
                     /locus_tag="OO568_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6643156.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          569 a          414 c          538 g          701 t
ORIGIN      
        1 accgttcctt aaattttccg gcaggtatgg aatatcggtt aacatattgt cgattgccca
       61 tatggagtga atgttgtgag gtagaggggg gagttcacta agaagattcc ctattgcgct
      121 aatctcttgt aaagaaaatg gtaaaggtgg taaaactgcc agcccattac atgatacatc
      181 taatgtttcc agtagttctg gtaaaacagg aagagaacat aattgattat ttgagacatg
      241 aagctccttc aaagatatgg ggagtgtggg tagtgtgatt agttgattgt gggacgcatt
      301 caataattta agtccttgag gcaaagcagg cagttcaata agtctgttat agctgacatt
      361 aagttgtgta agggacgcag gcaatgggga gattaagctt aaattatttt tacttatatt
      421 aattgattta attcccgggg ggatttcagg taatgttgtc aggcctaatt cagacaagtc
      481 caggttcgtc tcttggtttt gtagacatga tactagtcgc tgaaaagcga tgtctcgttg
      541 ttcttcttgt atgcggttgt ttttccattc agtccaatgg gttagataat tttcatatgc
      601 gcggctagtg tcgattgaat aagtggagaa cgaatttgaa attaatctgt gattgttatt
      661 tgtcgggagc ataaatatca gggcgtattc tttatgttga gaggtgttgg attctttttt
      721 atctgctttg caatgttatt ggttcccttc agcagtagac aggaacttct ggaggaggcg
      781 gatatatagt ttgtgtaagc atattaactc catatgttat atattgaaga acttctgctt
      841 tacactatcc taaactggat cggtttaatg taaaaaaacg atactttttg ggggaggggg
      901 caatttttcc ttaaggtatg aaaaaaggag ctgaagctat ataatagcta ttaatgccac
      961 tggtatcagt gaatcatgta tataaaaaac tgtgttaagg aggctgtcca ttagcggttt
     1021 tttattcttc tggaaccgcg gcatctttgc gtatcttttt gtcttctcac cgtctggtcg
     1081 gtgttttgct gagactgcta atttcctgtc tttgctagtg ctgtactggt actgcccaat
     1141 catgattggc gggggcgggc ggagttgaga ccgcagacac gtcgtatgca agaacgtgct
     1201 gcggctggct ggtgaacttc cgatagtgcg agtattgaat gatttccagc cgttactgat
     1261 tttacgtgct aattagtgaa caaaccactc gtcagcagac tcccaggtat ctttcagagt
     1321 ttcctgaaca aaagttttag ctgaatcttt atcggcggtg cgcgtaacag aaaggccatc
     1381 gttgctggtg gcttttacga tcacctctac atcgtcataa cgtttactga tgcgtcgggt
     1441 taattcttcc tttaacgcat ccacagcacc ggttggcatt ttagtcattt tctctttggc
     1501 tatgcagatt tcaatacgca taaaaatccc tctacactgt gtttttatac agtattattt
     1561 ttaactgtat ggataaacag tgtcaagagt gagggaaagt gattttttgg ggcatgcatg
     1621 gggcaaaaat atttgttgtg gggcattttg gggcatgatt gggacacttt gttacatatg
     1681 aactttgccg attttcatat gacatggcaa tccaacgctt tgattattct aaagaaaaca
     1741 catgctcttg ggcgttcttt agtgattttt aaaaatcccg tatcacgcag ttaaagtggc
     1801 gggcatactc ttcaaggctg gtgatcccca accgcaccca tttaggatgc gaccattgcg
     1861 gtagaccgat gtaaatcata acgcgtgaag gatctcttcc acgctacgca cacgggcgat
     1921 gcgcgggtag atatgattaa tgctgttatt gtgttgctcg gcgctggcgg cactacaggc
     1981 gtcttcggca atcaccagat taaagccaag ttcccaggca ttgcgggcgg tggattcaac
     2041 accgatattg gtcgagatcc cacataacac tattgtatcg atacctcggc ggcgcagttg
     2101 caactccaga tccgtaccgt aaaacgcacc ccactgacgt ttgatgattt cgatatcact
     2161 gtcagttgca cctaatgcag ctggatgttg ccaccagttt tcgggcaaca ctttagcggg
     2221 gg
//
LOCUS       DAJTYG010000228         2187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTYG010000228 DAJTYG010000000
VERSION     DAJTYG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.230"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(221..385)
                     /locus_tag="OO568_004068"
     CDS             complement(221..385)
                     /locus_tag="OO568_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643157.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTQPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="OO568_004069"
     CDS             1286..1570
                     /locus_tag="OO568_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6643158.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="OO568_004070"
     CDS             1558..2043
                     /locus_tag="OO568_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6643159.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          566 a          460 c          619 g          542 t
ORIGIN      
        1 ttcaaaatga tggacattat ttttgtggaa ccggaggtaa cagaccagac ggtttaaatg
       61 agccggttac tttaaacggg tgatggacag cacctcaaaa gttcagatgg attatatttg
      121 tcagcaatac cccgggtatt ttcgatttgc gaaatggatg gaacgacttg ctcaggggat
      181 agctgatgga gttactgaag taccgaagga ccactaaaaa tcacaggccg ggaatttcgg
      241 cgtttccggc gtaggtgtgc cacgaatgca tccagtggcg tgatgttgga tgcaatgctg
      301 tatgagagat gaggggctgc gtccagacgc agccttcaaa ccgccttgag ctgctgccgt
      361 taagagcggt ggtggtgagc gtcaaggtta aaaggcgcag aacatgtgtc aggtaggtgt
      421 tgcggagatg aacgaaagga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaagctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggtg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggttccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 ggtgaagctg tacttgatga ctggttaaag cagaaggggc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtatcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataa
//
LOCUS       DAJTYG010000229         2153 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTYG010000229 DAJTYG010000000
VERSION     DAJTYG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.231"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..769)
                     /gene="ipaH7.8"
                     /locus_tag="OO568_004071"
     CDS             complement(<1..769)
                     /gene="ipaH7.8"
                     /locus_tag="OO568_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR6643160.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1098..1277
                     /locus_tag="OO568_004072"
                     /pseudo
     CDS             1098..1277
                     /locus_tag="OO568_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1294..1984)
                     /locus_tag="OO568_004073"
                     /pseudo
     CDS             complement(1294..1984)
                     /locus_tag="OO568_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          633 a          394 c          492 g          634 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgcgagtcg
       61 acaatggatt ccctgaaata ttaatcctgt aacttgaatc taattgtgaa aaactctccg
      121 gaagagtggt tatctgatta aaatgaaaat aatattcctt ttgtctgata ttgtttctat
      181 cagaaaattg aggaaaagta accagattat tgcgctgagc acagagaatt tccagtttat
      241 ccggtaagtc gggtaatgtt gtcagcctgt tataataaac atgtaaaaat ttcaattttt
      301 cgggaagagc cggtaagtta tatagcctgt tattttcaac aaataatgat gttaaatggt
      361 ttggcaaaac aggaaggttt tccagttgat tagaacgaac acttaaagtt gttaatgact
      421 caggaagttc tggtatcaca gaaagcctgt tattatcagc attaagttct ttgagaaatg
      481 caggcagctt tggcaatgat gttaatttat tattttctac aatcaaagca gaaatatgct
      541 ttggtaattc gggaagagac gtcaaattta ggtctgataa atttaatact gcttcttgat
      601 tctgaaagca ctgggagagt ctgttaaaag caatgcctcg ctcttccccg ggagaagagt
      661 tcttttccca ttcagtcagg attctcagat aatgttcatt actggttgag tttgagttaa
      721 tagaggggga gcaagaaact gatgagtgtg tattatttac agagaacata atgctaggtc
      781 aaccttatat ttgtgagaat aaatatttaa aattgtatat ttatacaaat ttcacttacc
      841 atttccgata ttaagtttta atagtttcca ttttacacat aacagaagtt atgttgtaat
      901 ttatttatgg aggttttcta tggaattctt ttttattgtg actgcagtca ataataacac
      961 tgaattcaac attccagtga gcaaggaaat gtaatccgag taagtatatt tgttttacat
     1021 catattttag tcaagctcat ttattcctat tatagaatga taaattaaaa ttactgtaaa
     1081 aaaacgatac acatttaata cgagggaggc cggcagaaaa acggcttgcg gtaaggcagg
     1141 agaaatgtgt gccgcgaatg aagtccttgt atgactggat aaagctccag aggaaaactt
     1201 tgtcaaacca tgctgaaatg gtaaaggcgt tcaactatat cctgaatcac tggaatgcac
     1261 tgaatgagtt ttgccgggta gtacttccaa tcattagaat atgtgctttt caatgaagga
     1321 accgatgact ttttcgtgga tttccacgga acgcgagaag cagattgttt tacgtgccag
     1381 ccgcttgatg cgggggcgga gggtcaggtt gttacgttca tacgttgcgt gaagattttg
     1441 ccggtcaggt gtttctcctc cggcacttct ctggcgtagc tgccccagtc atcgctggtc
     1501 accatgccga tacagaacgg agtcaacagg gctaacagtt cccgacaggt ctcatcattc
     1561 cgtggcccgc aagtgtaagc caacacaccg ccagttttag tgttgtacgc ataccagggc
     1621 cagtgttgtt gcgccttgct gccaacgaag cgccactgct catcaagttc acagataagg
     1681 gcaacatcag cgtgagcgac tggcgcagat gttatccgcc ggggcgagag ctttttaatg
     1741 tccgtatgac ggtattgatg ccgattttca gtgttctggc ggtataacag ccccctgcgc
     1801 cgttatgtgc catttcaacg atctgctcct taacgccgag ttttctggct tcataagagt
     1861 aagtgagctg gaaaacacgt ttacatgagc gacaacggaa acgctcatgc cgggaacaac
     1921 tacggccatg tcgataaact tcatcggaat tacagcgcgg acaatgaaca gtaacggtag
     1981 tcatggaaaa cctcaaatac cagtagatta ccagaaactt ctatcgattg aagacattac
     2041 cttttgccgc aataggtggg gtaacattgg tgaaaacgca ttacgccggg tggcagttgg
     2101 aaaaaaatta tcttttttcg gctctgatcg tggtggagaa aatgcggcaa taa
//
LOCUS       DAJTYG010000230         2140 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTYG010000230 DAJTYG010000000
VERSION     DAJTYG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.232"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..646)
                     /gene="yidZ"
                     /locus_tag="OO568_004074"
     CDS             complement(<1..646)
                     /gene="yidZ"
                     /locus_tag="OO568_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6643161.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
     gene            complement(621..1796)
                     /gene="mdtL"
                     /locus_tag="OO568_004075"
     CDS             complement(621..1796)
                     /gene="mdtL"
                     /locus_tag="OO568_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6643162.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1928..>2140)
                     /locus_tag="OO568_004076"
     CDS             complement(1928..>2140)
                     /locus_tag="OO568_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6643163.1"
                     /translation="IYGIGGARLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIP
                     AIVILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          599 a          571 c          550 g          420 t
ORIGIN      
        1 aggtgtcgct ctgttcccag caaatgctga tatgcggata acgtaaaaag gtgtccagat
       61 tccacgtttc atgcagtgcc ggatgatctt tgcgtaacca gacgcagggt acatcactaa
      121 acagcacttc ataatcaatg gctaacggta gcgagcttaa cagctcccgc gagcgaggat
      181 ggctttcgcg accggaaaaa ccaatatcca cttcaccacg agtaatggca tctaaggaat
      241 cgtaatccca gttacgtagt ttgatggtcg cctgcgggta acgttggtag atccgtttcg
      301 acagcgcatt aagcatgatc atcatcagcg gcgattccgc cgccaactca aacttcaggc
      361 cgcgcggtgt ctggtggtgc ggtttatcca gcagcagatt gctcatttgc atccattccg
      421 ccagattttg ctccatgctg accattagct gtgtgggcga cagccccagc ggtgagttca
      481 caaagagcgg gtcgtcaaac cacgctctta actttgccag cgacttactc accgctgaag
      541 gggtcacgtt catccgcttc gccgctttgg tcacgctgcg ctcctgcatc agcagttgca
      601 gacagagcag cagattgaga tcaagcgtgg tgatggattt cttcatgagc ggcaacgggg
      661 cgtccaggcg cgacgaacat aatcagcaac aggctcacta tgctacaggc aatcagaatc
      721 ccgatcagca tattccatgc gccgatacca accaccgcgg ccagccaaat ccacagtgac
      781 gaaccgcaaa cctgcgcaat acctaaggtc gagctggcta cgcccgcgcg taatgaaaat
      841 ggtcctaacg cctgactcat cgctacgcca aaacctaccg agaaaccggc gcaaatcagc
      901 gtgataccaa acagagaaac cgcatgggaa ggtgaaacgg caagggtgat ccccgccgca
      961 aggaataaca cctgcgaggt gatcatcaac gtacgtggct taaaaattcc cagcgcaaat
     1021 ggcgtcgaga atgaaaaggt catgctgacg ccagcggtca gcgccataat ggtggcgtat
     1081 tctccgcgct caaaccccat gatttccatc agcaataccg gcgatgtatt gacgaaagtg
     1141 aggatcaccg aaacgctgag ggtggtgata acaacacggc tgaggaaaaa acgattaagc
     1201 agcgactcgc tattttctcg tggtttgtcc gaagcagcgg gggacgctgg gcgcgtttct
     1261 tttaaaataa acaaagacaa catcagtacc gcgatgccca tcattgccat cgcccagaac
     1321 agactctgcc acgggaattt aagcataatc agatgtccga gcactggcgc taacaccgga
     1381 atgatgcagg taataccgtt gagtaatgac agcactttag cccgacgtcg atcatccagc
     1441 gtgtcgcgca aaatggcgaa cgccaccacg taacaacagc ctgcgcccaa cccctgtaga
     1501 aatcggcctg caagaaataa cgtgctggtt tcagccagtg aacagaacac cgaggcaata
     1561 ataaatagcg ccgcgccggg tatggcgacc ggctttctcc ctgaacgatc ggccacttta
     1621 ccggcaaata gcatcgcagc tgccatcccc gccagatata cggagaacgc aatatgcaac
     1681 tgcgcttcgc tggcattgag atcggcggcg atgcgcggta aaccaacgag gtacatatca
     1741 atcccggcgg gataaagtaa aaccagggca aaactacaaa tcaaaaagcg ggacatgggc
     1801 taaagcgtcc tgatagtaag gtgacgcaaa gcatacgtgg tgaaggagga taaggcgagt
     1861 tgccatttgg acataagtga tttcctgaga ggcaagaagc cagcgaatgg ctggcttctt
     1921 gaaggattta gccaaattta ggtaacacgt taaagacgtt gccgaaccag cacaaaatca
     1981 cggttatacc aaagagaatg acaatcgccg gaataagatt gccgccccag accgtgaaca
     2041 tctgattggg aaacttcttg cgagctttga ttgcaagcac tgcgggaata atgaccgccc
     2101 agatggtggc gcacagcctg gcaccgccga tcccgtaaat
//
LOCUS       DAJTYG010000231         2135 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTYG010000231 DAJTYG010000000
VERSION     DAJTYG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.233"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..>2135
                     /locus_tag="OO568_004077"
     rRNA            <1..>2135
                     /locus_tag="OO568_004077"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          586 a          467 c          655 g          427 t
ORIGIN      
        1 gacgtgctaa tctgcgataa gcgtcggtaa ggtgatatga accgttataa ccggcgattt
       61 ccgaatgggg aaacccagtg tgattcgtca cactatcatt aactgaatcc ataggttaat
      121 gaggcgaacc gggggaactg aaacatctaa gtaccccgag gaaaagaaat caaccgagat
      181 tcccccagta gcggcgagcg aacggggagc agcccagagc ctgaatcagt gtgtgtgtta
      241 gtggaagcgt ctggaaaggc gtgcgataca gggtgacagc cccgtacaca aaaatgcaca
      301 tactgtgagc tcgatgagta gggcgggaca cgtggtatcc tgtctgaata tggggggacc
      361 atcctccaag gctaaatact cctgactgac cgatagtgaa ccagtaccgt gagggaaagg
      421 cgaaaagaac cccggcgagg ggagtgaaaa agaacctgaa accgtgtacg tacaagcagt
      481 gggagcacgc ttaggcgtgt gactgcgtac cttttgtata atgggtcagc gacttatatt
      541 ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa ctgggcgtta
      601 agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg aaggttgggt
      661 aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga cttgtggctg
      721 ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct atttaggtag
      781 cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt catcccgact
      841 taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac acacggcggg
      901 tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt cccaaagtca
      961 tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc ttagaagcag
     1021 ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa
     1081 cggggctaaa ccatgcaccg aagctgcggc agcgacgctt atgcgttgtt gggtagggga
     1141 gcgttctgta agcctgtgaa ggtgtgctgt gaggtatgct ggaggtatca gaagtgcgaa
     1201 tgctgacata agtaacgata aagcgggtga aaagcccgct cgccggaaga ccaagggttc
     1261 ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa ggcgaggccg aaaggcgtag
     1321 tcgatgggaa acaggttaat attcctgtac ttggtgttac tgcgaagggg ggacggagaa
     1381 ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt gtaggctggt tttccaggca
     1441 aatccggaaa atcaaggccg aggcgtgatg acgaggcact acggtgctga agcaacaaat
     1501 gccctgcttc caggaaaagc ctctaagcat caggtaacat caaatcgtac cccaaaccga
     1561 cacaggtggt caggtagaga ataccaaggc gcttgagaga actcgggtga aggaactagg
     1621 caaaatggtg ccgtaacttc gggagaaggc acgctgatac gtaggtgaag cgacttgctc
     1681 gtggagctga aatcagtcga agataccagc tggctgcaac tgtttattaa aaacacagca
     1741 ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct gcccggtgcc ggaaggttaa
     1801 ttgatggggt tagcgcaagc gaagctcttg atcgaagccc cggtaaacgg cggccgtaac
     1861 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacctg cacgaatggc
     1921 gtaatgatgg ccaggctgtc tccacccgag actcagtgaa attgaactcg ctgtgaagat
     1981 gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct ttactatagc ttgacactga
     2041 acattgagcc ttgatgtgta ggataggtgg gaggctttga agtgtggacg ccagtctgca
     2101 tggagccgac cttgaaatac caccctttaa tgttt
//
LOCUS       DAJTYG010000232         2108 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTYG010000232 DAJTYG010000000
VERSION     DAJTYG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.234"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            129..1166
                     /gene="curA"
                     /locus_tag="OO568_004078"
     CDS             129..1166
                     /gene="curA"
                     /locus_tag="OO568_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6643164.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVMGVAGGAEKCRHATEVLGFDVCLDYHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1364..2029
                     /gene="mcbR"
                     /locus_tag="OO568_004079"
     CDS             1364..2029
                     /gene="mcbR"
                     /locus_tag="OO568_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6643165.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          582 a          449 c          563 g          514 t
ORIGIN      
        1 ggcaaatcca acggattcct cgatgaaatt attgcgtgtt gcagagggga tgtgacggct
       61 gcaaacaaaa ccaatcccgt gagcctgcta ctattgatgg attcatgcca aaagcacagg
      121 agagcattat ggggcaacaa aagcagcgta atcgacgttg ggttctggcc tcgcgtccac
      181 atggcgcacc ggttccggag aatttccgtc ttgaagaaga tgatgtcgcc acaccgggtg
      241 aaggacaggt gttactgcgc acagtttatt tgtccctgga cccgtatatg cgtggacgta
      301 tgagcgatga gccatcttat tcaccgccgg ttgatattgg cggcgtgatg gtcggcggta
      361 cggtgagccg tgtcgtggag tcgaatcatc ctgattatca gcctggcgac tgggtgctgg
      421 gctacagtgg atggcaggac tatgacatat ccagtggtga tgacctggtg aaacttggcg
      481 atcatccgca aaatccatcg tggtcgctgg gtgtgctggg gatgccaggc tttaccgctt
      541 atatggggct gctggatatc ggtcagccta aagagggcga aacgttggtg gtagctgcgg
      601 cgacaggacc agtgggggcg acggtggggc aaatcggcaa acttaaaggt tgcagagtga
      661 tgggggtagc cggtggcgcg gaaaaatgcc gccatgctac cgaggtgtta ggcttcgatg
      721 tttgccttga ttaccatgcg gacgattttg ccgaacaact ggcgaaagcg tgcccaaaag
      781 gtattgatat ctattatgaa aacgtgggcg gcaaggtatt tgatgcagtg ctaccattac
      841 ttaatacatc tgcgcgcatt cccgtttgtg gcttagtgag cagctataac gctacagagc
      901 taccacccgg tccggatcgt ttacccctgt tgatggcaac ggtgctgaaa aaacgtattc
      961 gcttgcaagg gtttattatc gctcaggatt atggtcaccg catccatgag tttcagaagg
     1021 agatggggca atgggtgaaa gaggataaaa tccactaccg cgaagatatt actgatggtt
     1081 tagagaatgc gccacagacg tttatcggcc tgctgaaggg taaaaacttc ggcaaagtgg
     1141 tgatccgcgt cgcgggtgat gattaaagtt tcaacggcgg cgtaagccgc cataaaaatc
     1201 caggcaaaaa gcttattttc agctttaatt aactaaccac gtcattgttc tgctgctaat
     1261 aacaacaaag gtgggtggtt tatgattatt cactttaata cactaggtga actccttctg
     1321 ccatgcaggc agggttggac agaaaacacg cgaaggaatt aaaatgcctg gaacgggaaa
     1381 aatgaaacat gtcagtttga ctctgcaggt tgagaacgac ctgaaacatc agcttagtat
     1441 tggcgcatta aaacctggcg cacgcctgat tactaaaaat ctggcggagc aattaggtat
     1501 gagtattaca cctgtgcgtg aagcattatt acgtctggtt tcggtgaatg cgctttctgt
     1561 cgcacctgca caagcattta cagtaccgga agtgaggcaa agtcagttag acgaaatcaa
     1621 tcggatccgc tacgaactgg aattaatggc agttgccctg gctgttgaaa acctctcctc
     1681 gcaagacata gcagaacttc aggaattgct cgggaaatta cagcaggcgc aagaaaaggg
     1741 cgacatggaa caaatcatta atgcgaacag gctatttcgc ttagcgattt atcatcgctc
     1801 aaatatgccc atcctgtgtg agatgattga gcaactgtgg atcaggatgg gacctgcttt
     1861 acattatctt tatgaagcga ttaacccagc ggaattacgg gagcgcatag aaaactatca
     1921 tctattactc gctgcgttaa aagcaaaaga caaagaggga tgcaaacatt gtcttgctga
     1981 aattatgcaa caaaatattg ctattttata ccagcaatac aatcgttaaa ttaaatgccc
     2041 ggcaaatacc gggcatcaag taattactca aatcgccacg caatattcat gccgacacca
     2101 taatttcg
//
LOCUS       DAJTYG010000233         2099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTYG010000233 DAJTYG010000000
VERSION     DAJTYG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.235"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(60..1025)
                     /locus_tag="OO568_004080"
     CDS             complement(60..1025)
                     /locus_tag="OO568_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCR6643166.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVGLWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1889..>2099)
                     /locus_tag="OO568_004081"
     CDS             complement(1889..>2099)
                     /locus_tag="OO568_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643167.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          580 a          550 c          440 g          529 t
ORIGIN      
        1 aaaaaaaata aactaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cgacgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagccct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataaccg caattttaac ccccaaaaca agcacccccg aacgactacc ccaacctttt
     1381 ccacgcaccc gatcaccaaa acagggaata aaaacaaaac cctgtcttac aggtggatgg
     1441 cctcacttcg tgaaaccatc caccccgcat aagttttttc atcggtggtg tcgtcaatca
     1501 ctaaatcgga accccaaagg ggacccccac gatttagcat ttgacgagat taactctcgg
     1561 agatggcatc atgcttttaa cggactttaa aaaacggtga acggatgcgg attttaatcc
     1621 gtgtctgtga ggcgttgagc aacgagcaaa gcgaggcggt agaaagagag ccagcggcag
     1681 gctcaggacg ataatccggg gaaaatgtac cactcgcaga gtgttaacat ttgacatcgg
     1741 attgtctatc ctgcattggt gtcgagttgg caaggctgag gataatgctg aggataatgc
     1801 tgaggataat gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc
     1861 tcagcctgcc gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga
     1921 taccgtttaa atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat
     1981 ataaaattaa ttcatcttca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta
     2041 atccatcaat aagcaaaata aggtttttaa acctgtcagg aatcactgct tcacttctt
//
LOCUS       DAJTYG010000234         2068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTYG010000234 DAJTYG010000000
VERSION     DAJTYG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.236"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..692
                     /locus_tag="OO568_004082"
     CDS             <1..692
                     /locus_tag="OO568_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6643168.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLLGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     EMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAIYLLAGCLISAM
                     TALLMKDNQRS"
     gene            794..>2068
                     /gene="amn"
                     /locus_tag="OO568_004083"
     CDS             794..>2068
                     /gene="amn"
                     /locus_tag="OO568_004083"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6643169.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLC"
BASE COUNT          485 a          511 c          496 g          576 t
ORIGIN      
        1 tactgggagc attgtgggtg cgcaatggca tggaggagtc cgcggaattt gaacaacagc
       61 aacataatca agcggccgcg aaaaaacgca tcccggttat cgaagctctg ttacgacatc
      121 ccggtgcttt cctgaagatt attgcactac gactgtgcga gttgctgacg atgtacattg
      181 ttactgcctt tgcacttaat tattcaaccc agaatatggg gttaccgcgc gaacttttcc
      241 ttaatattgg tttgctgtta ggtggattaa gctgcctgac aattccctgt tttgcctggc
      301 ttgccgatcg ttttggtcgg cgcagggttt atatcacagg cgcgttgatc ggaacgttga
      361 gcgcatttcc tttctttatg gcgcttgaag cacaatctat tttctggata gttttcttct
      421 ccataatgct ggcaaacatt gcgcatgaca tggtggtgtg tgtgcaacaa ccgatgttta
      481 ccgaaatgtt tggtgccagt tatcgctaca gtggtgctgg agtcggttat caggttgcca
      541 gtgtggttgg cggtggattt acacctttta ttgccgctgc actcatcact tactttgccg
      601 ggaactggca tagcatcgcc atttatttgc tggctggatg cctgatttct gcaatgaccg
      661 ctttattgat gaaagacaat caacgctcgt gatagcctgc cgaagatcat ccgatcttcg
      721 ccttatactt ttgtttcaca ttcctgtgac atactatcgg atgtgcggta attgtatgga
      781 acaggagaca cacatgaata ataagggctc cggtctgacc ccagctcagg cactggataa
      841 actcgacgcg ctgtatgagc aatctgtagt tgctttacgc aacgccattg gcaaatatat
      901 tacaattggc gaattacctg atgaaaacgc ccgtaaacaa ggtctttttg tctatccatc
      961 actgaccgta acctgggacg gtagcacaac aaatcccccc aaaacgcgcg catttggtcg
     1021 ttttactcac gcaggcagct acaccaccac gattactcgc cctactctct ttcgttcgta
     1081 tctgaatgag caactaacgt tgctttatca ggattatggt gcgcatatct ctgtgcaacc
     1141 ctcgcaacat gaaatccctt atccttatgt catcgatggc tctgaattga cacttgatcg
     1201 ctcaatgagc gctgatttaa ctcgctactt cccgacaaca gaactggcgc aaatcggcga
     1261 tgaaactgca gacggcattt atcatccaac ggaattctcc ccgctatcgc attttgatgc
     1321 gcgccgcgtc gatttttccc tcgcacggtt gcgccattat accggtacgc cagtagaaca
     1381 ttttcaaccg ttcgtcttgt ttaccaacta cacacgttat gtggatgaat tcgttcgttg
     1441 gggatgcagc cagatcctcg atcctgatag tccctacatt gccctttctt gtgctggcgg
     1501 gaactggatc actgccgaaa ccgaagcgcc agaagaagcc atttccgacc ttgcatggaa
     1561 aaaacaccag atgccagcat ggcatttaat taccgctgat ggtcagggta ttactctggt
     1621 gaatattggc gtgggaccgt ccaatgctaa aaccatctgc gatcatctgg cagtgctacg
     1681 cccggatgtc tggttgatga ttggtcactg tggcggatta cgtgaaagcc aggccattgg
     1741 agattatgtc cttgcacacg cttatttacg cgatgaccac gttcttgatg cggttctgcc
     1801 gcccgatata cctattccga gcattgctga agtgcaacgt gcgctttatg acgcaaccaa
     1861 gttagtcagc ggcaggcctg gtgaggaagt caaacagcgg ctgcgtacag gtactgtggt
     1921 aaccacagat gacaggaact gggagttacg ttactcagct tctgcacttc gttttaactt
     1981 aagccgggcc gtagcaattg atatggaaag tgcaaccatt gccgcgcaag gatatcgttt
     2041 ccgcgtgcca tacgggacac tactgtgt
//
LOCUS       DAJTYG010000235         2042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTYG010000235 DAJTYG010000000
VERSION     DAJTYG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.237"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            14..487
                     /locus_tag="OO568_004084"
     CDS             14..487
                     /locus_tag="OO568_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6643170.1"
                     /translation="MRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVADPNLDELYQV
                     YSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGKGLAKKLALMA
                     MEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCEVRMLREL"
     gene            complement(533..949)
                     /gene="rraB"
                     /locus_tag="OO568_004085"
     CDS             complement(533..949)
                     /gene="rraB"
                     /locus_tag="OO568_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6643171.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1111..>2042
                     /gene="argF"
                     /locus_tag="OO568_004086"
     CDS             1111..>2042
                     /gene="argF"
                     /locus_tag="OO568_004086"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6643172.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAA"
BASE COUNT          511 a          502 c          514 g          515 t
ORIGIN      
        1 gcaatcacct gtaatgcgtc gcctgacgct gcaggataat cctgctatcg ccagcgtcat
       61 ccgtcaggtt tacgcagagt atggtcttac cgccgataaa ggctacaccg ttgccgatcc
      121 gaatctcgac gaactgtatc aagtgtacag ccagcctggc catgcttatt gggtggttga
      181 atacaatggc gaagtggtcg gcggcggcgg gattgcgcca ttaacaggta gtgaagcgga
      241 catttgcgaa ctgcagaaaa tgtactttct tcccgctatt cgcggtaaag ggctagcgaa
      301 aaaactggcc ttaatggcga tggagcaggc tcgagagatg ggcttcaaac gctgctatct
      361 ggaaacgacc gcttttttaa aggaagccat tgcgctttat gagcatttgg gctttgagca
      421 tatcgactat gcgcttggct gcacgggcca tgtcgattgt gaagtgcgga tgctacgcga
      481 actctaaaaa aatgcccgct agtatgtgct atcgggcatt ttaatcaatt aattagtggc
      541 gaaccccgtc atcgtcttca tcgacaaaat cttcatcgtc gccatcttcg ccgttaggat
      601 cttcaaagta agtgccccaa ccgtcgtact caacgtcaaa tttctccgcc agcgtcatca
      661 gttgttcaac ctgggcatcg atcagatcgg cattcaacgc gcactcgctg aggatgtcgc
      721 agcaaatcac gatatcacca tcttcaactt ccagctcttc tggatcggtc acttcgtaac
      781 cgagtttaaa cgcttcaact gccgcttttt ccagagtttc cagatcgtct gcggaaagat
      841 ggtgttcgat ggtgtacagc gcgtccggat cgctgccatc ttccagtaat tcttcaataa
      901 tcaaacgtgt ttcttcacgc tgttcttcca gttgttccgg gtttgccatg gctcattcct
      961 cttaatgtcc tgccgatact cttattgtca cacaccactg acattgcctc cacctttgtg
     1021 acaaagattt atgctttaga cttgcaaatg aataatcata cacataaatt taattttaat
     1081 tcattgaggc gttagccaca ggagggatct atgtccgggt tttatcataa gcatttcctg
     1141 aaattactcg atttcacgcc agctgaactc aacagcctgc tgcagttagc cgcgaagctg
     1201 aaagctgata agaaaagcgg taaagaagaa gccaaactca ctggtaaaaa catcgcgctc
     1261 atcttcgaaa aagactcgac tcgtacccga tgctctttcg aagttgccgc atatgaccag
     1321 ggtgctcgcg ttacttatct tggcccaagc ggtagtcaga ttggtcataa agagtcgatt
     1381 aaagacactg cccgcgtgct tggtcgcatg tatgacggta ttcagtatcg cggctatggt
     1441 caggagattg tcgaaacact ggcggaatac gctggcgtgc cggtatggaa cggcctgacc
     1501 aatgagtttc accccacgca gttgctggcg gatctcctca ccatgcagga gcatttaccc
     1561 ggtaaagcat tcaacgaaat gacgctggtc tatgcaggtg acgcacgtaa caacatgggc
     1621 aattcgatgc tcgaagctgc ggcgcttacc ggtctggatt tgcgtctggt cgcgccacag
     1681 gcatgctggc cagaagctgc gctggttacg gaatgccgcg ccctggcaca acaaaatggc
     1741 gggaatatta cgctgacgga agatgtagct aaaggagttg aaggtgctga ctttatctat
     1801 accgatgtat gggtgtcgat gggtgaagca aaagaaaaat gggctgaacg cattgcatta
     1861 ctgcgtgatt atcaggtgaa cagcaagatg atgcagttga ccggtaaccc ggaggtcaaa
     1921 ttcctccact gcctgcccgc gtttcatgac gaccaaacga cgcttggcaa aaaaatggcg
     1981 gaagagtttg gcctacatgg cggaatggaa gtgactgatg aggtcttcga gtctgccgcc
     2041 ag
//
LOCUS       DAJTYG010000236         2032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTYG010000236 DAJTYG010000000
VERSION     DAJTYG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.238"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..362)
                     /locus_tag="OO568_004087"
     CDS             complement(<1..362)
                     /locus_tag="OO568_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6643173.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /locus_tag="OO568_004088"
     CDS             complement(429..1643)
                     /locus_tag="OO568_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR6643174.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVMMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          634 a          492 c          412 g          494 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt gcgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcat gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgactactac catacaagag agaaaaagcg attattcaac cgtgttcaca
     1741 ttaaaaacaa aaatttccac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttaaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aa
//
LOCUS       DAJTYG010000237         1998 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTYG010000237 DAJTYG010000000
VERSION     DAJTYG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.239"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(89..1501)
                     /gene="pykF"
                     /locus_tag="OO568_004089"
     CDS             complement(89..1501)
                     /gene="pykF"
                     /locus_tag="OO568_004089"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6643175.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIQII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          501 a          456 c          483 g          558 t
ORIGIN      
        1 tgatttttta ttgcttctgg ttatcgatta aataaaaaaa gcgcccatca gggcgcttcg
       61 atatacaaat taattcacaa aagcaatatt acaggacgtg aacagatgcg gtgttagtag
      121 tgccgctcgg taccagtgca ccagaaacca taactacgac gtcacctttg tgtgccagac
      181 cgctctgcag agccagttct ttacccagac ggtagaaatc atcagtagaa gtgatctctt
      241 taaccagctg cggcacaacg cctttgctca gtaccaactg atgagccgtt ttttcgttgg
      301 tggtcagtgc caggatggtg gcatccggga agtatttacg tactgcgcga gcagatttac
      361 cgccctgggt agcaaccacg atcagcggag catccagttt ttcagcagtt tcaacggcac
      421 cacggcatac cgcttcggtg atgcgcagtt tacggttgtc attgttgaac tcgagacggc
      481 tgttcatcac gcggtcggta cgttcgcaga tggtcgccat gatagaaacc gcttccagcg
      541 ggtatttacc ttttgcggat tcaccagaca gcatcactgc gtcagtaccg tcgaggatgg
      601 cgtttgcaac gtcaccggct tctgcgcgag tcgggcgtgg gtttttgatc atggaatcca
      661 gcatctgggt cgcagtgata acgactttac gtgcacggat acatttttcg atcatcatct
      721 tctgggcgaa gataacttct tctaccggga tttctacacc caggtcgcca cgcgcaacca
      781 tgatgccgtc agaggcttcg aggatttcgt cgaagttgtt gaggccttcc tggttttcga
      841 ttttggagat gatctggatg ttttcgccgc cgtgcgcttt caggtgctca cggatttcga
      901 taacgtcaga acgcttacga ataaaggaag cagcaacaaa gtctacgcct ttttcgcaac
      961 caaagatcag gtcctgtttg tctttttcag ccagtgctgg cagagcaatg gaaacgccag
     1021 gcaggttcac acctttgttt tcgcccaggt caccgttgtt cagcacttta cagataactt
     1081 tgttaccttc aatggcggta acttccatac cgatcagacc atcgtcaacc agtacggtgt
     1141 tgccaacaga caggtcagta gtgaaacctt catacgttac cgcaaccatt tcgctgttgc
     1201 cgataacaga tttatcagtg gtgaaagtaa aggtctgacc agctttcaga gaaacatcgt
     1261 taccgccttc cagtttcatg gtgcggattt ccggaccttt ggtatcaagc aggatagcgg
     1321 cggttttacc agttttgctc atcacgttgc gcagattctg aatgcgctga ccgtgttctg
     1381 catagtcacc atgagagaag ttcagacgca taacgttcat gccggcgtcc agcattttag
     1441 ctaacatctc ttcagattcg gttttcggtc cgatggtgca aacaattttg gtctttttca
     1501 tgacagtctt agtctttaag ttgagaagga tgggagaaac ttgctttctg ggcgcactgc
     1561 cggaaagcga atccggtatt acgaaagtgg tggtgcctcg ctctaaggat aggtgacatc
     1621 gaaaaagcgt gcagaggaat gtgtgcttgt gtctcagccc aacgtgagtg cctgtttttg
     1681 agtcgttgag ttctacagtc caaagtgctg aaaccattca agagtcaatt ggcgcgcatt
     1741 atacgctgaa attaatccaa aaggaaaaca aaaacagcgt ttcaaaaaat atacaacttg
     1801 ttagacaata aattgttatc aaagatttaa cgttccaggg aaaaggttac gcaaaaactt
     1861 cgctgtttgc gcaacgtctg gtgccgaagt tgtcagtgtg aggataagtg aaagttcatc
     1921 cggtgtataa atatatcatc gctcaattgc tacagttaaa ataatttatc gcgttatttg
     1981 tctgattttg tttgattt
//
LOCUS       DAJTYG010000238         1946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTYG010000238 DAJTYG010000000
VERSION     DAJTYG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.240"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..258)
                     /locus_tag="OO568_004090"
     CDS             complement(<1..258)
                     /locus_tag="OO568_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643176.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            283..522
                     /gene="relB"
                     /locus_tag="OO568_004091"
     CDS             283..522
                     /gene="relB"
                     /locus_tag="OO568_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR6643177.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            522..809
                     /gene="relE"
                     /locus_tag="OO568_004092"
     CDS             522..809
                     /gene="relE"
                     /locus_tag="OO568_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR6643178.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            881..1036
                     /gene="hokD"
                     /locus_tag="OO568_004093"
     CDS             881..1036
                     /gene="hokD"
                     /locus_tag="OO568_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6643179.1"
                     /translation="MKQQKAMLIALIVIYLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1254..1504
                     /locus_tag="OO568_004094"
                     /pseudo
     CDS             1254..1504
                     /locus_tag="OO568_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1571..1849
                     /locus_tag="OO568_004095"
     CDS             1571..1849
                     /locus_tag="OO568_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643180.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
BASE COUNT          521 a          439 c          496 g          490 t
ORIGIN      
        1 tgacatgcta aagcaaaaaa tagatgaata agttgagttg tgcatatgta gcctgaccgt
       61 cacaaagtat atggtgtctg taccagtaag atgatggccg gactctttaa aaaagagctg
      121 acctgcacaa tacaggatgg acttagcaat ggctgctcct ggcacaaagc ggacagtgat
      181 caccgttctt acgactactt tctgacttcc ttcgtgactt gccctaagca tgttgtagtg
      241 cgatacttgt aatgacattt gtaattacaa gaggtgtaag acatgggtag cattaacctg
      301 cgtattgacg atgaacttaa agcgcgttct tacgccgcgc ttgaaaaaat gggtgtaact
      361 ccttctgaag cgcttcgtct catgctcgag tatatcgctg acaatgaacg cttgccgttc
      421 aaacagacac tcctgagtga tgaagatgct gaacttgtgg agatagtgaa agaacggctt
      481 cgtaatccta agccagtacg tgtgacgctg gatgaactct gatggcgtat tttctggatt
      541 ttgacgagcg ggcactaaag gaatggcgaa agctgggctc gacggtacgt gaacagttga
      601 aaaagaagct ggttgaagta cttgagtcac cccggattga agcaaacaag ctccgtggta
      661 tgcctgattg ttacaagatt aagctccggt cttcaggcta tcgccttgta taccaggtta
      721 tagacgagaa agttgtcgtt ttcgtgattt ctgttgggaa aagagaacgc tcggaagtat
      781 atagcgaggc ggtcaaacgc attctctgaa ccaaagcatg acatctctgt ttcgcaccga
      841 aggtgacact tctgctttgc gttgacagga gaagcaggct atgaagcagc aaaaggcgat
      901 gttaatcgcc ctgatcgtca tctatttaac cgtcatagtg acggcactgg taacgaggaa
      961 agacctctgc gaggtacgaa tccgaaccgg ccagacggag gtcgctgtct tcacagctta
     1021 cgaacctgag gagtaagaga cccggcgagt gagaaatccc tcgccacctc tgatgtggca
     1081 ggcatcctca acgcacctgc acttaacccg cttcggcggg tttttgtttt tattttcaac
     1141 gcatttgaag ttctggatgg tgccggaata gaatcaaaaa tacttaagta gcgcgcaggg
     1201 ataaaaggga tggaccccga acaggggagt gctacttatc tggaaggatt ctgatgatga
     1261 acatcgaaga actgcgtaaa attttttgtg aagatggcct ctatgctgtg tgcgttgaaa
     1321 atggaaatat tgttagtcat taccgcattg tgtgtttgca aaaaatgggg ctgcgttaat
     1381 taattttgtg gatgcccgag tgacggacgg atttatcttg cgcgacggtg agtttgtcac
     1441 ttcattacag gcattgaaag agaccggaat aaaagctggc ttttctgctt tttcagaaga
     1501 ataaactcat ctacaatctt gcgcggggct gaactcccgc tgagtaacac cgtgccaccg
     1561 gagaaaaccg atggcacgca acgcaaaata ttacaatcat gataattcga ccgttcttgc
     1621 ccacacgcac gagcggtatt ctcacgcatt taagtcagac tggtaccagc atccctcatg
     1681 cactgaagaa caggccgaat ggctcattca gtgttaccgc aggcgcggat gcgaggttaa
     1741 aaaagccctt agcctcgact accgtcactg gataatctcc gtcaggctcc cttactccga
     1801 acagccaccg cgtccgtccc gcacattcca gcaacggatc tggaggtaac gtgcgggtat
     1861 tacttcgacc tgttctggtt ccggaactcg ggctggtggt ccttaagccc ggtcgtgaat
     1921 ccatgccggt attccacaat acccgg
//
LOCUS       DAJTYG010000239         1922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTYG010000239 DAJTYG010000000
VERSION     DAJTYG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.241"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(13..363)
                     /gene="chbA"
                     /locus_tag="OO568_004096"
     CDS             complement(13..363)
                     /gene="chbA"
                     /locus_tag="OO568_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR6643181.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(422..1772)
                     /gene="chbC"
                     /locus_tag="OO568_004097"
                     /pseudo
     CDS             complement(422..1772)
                     /gene="chbC"
                     /locus_tag="OO568_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(1857..>1922)
                     /locus_tag="OO568_004098"
     CDS             complement(1857..>1922)
                     /locus_tag="OO568_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; involved in N,N'-diacetylchitobiose
                     transport; protein IIA transfers a phosphoryl group to IIB
                     which then transfers the phosphoryl group to the sugar;
                     IIC forms the translocation channel for the sugar uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6643182.1"
                     /translation="KVDGLGVLKAAVAAIKKAAAN"
BASE COUNT          535 a          496 c          425 g          466 t
ORIGIN      
        1 atcatcgact ccttatgcct tcagtttttc atgaagctca attaattcag taatcagttc
       61 acgcgcaagc atggacgtca ttaaatgatc ctgagcgtgg accagcacca gactcacttt
      121 catcttacct tcgcccgcat cgccttcaat cagtttcgtc tgtaccagat gcgcttcatt
      181 caatgccatc cgtgactgat ccatcatggc tttcgctgcg gcaaaatcgc cctgcttcgc
      241 ctgtttcaat gcggcatacg ccaggctacg cgcttgtcca gagttgatga taagccccat
      301 caccacttct tccagctctt cagcttctgt ttgcgtatca ggaatattat cgagatccat
      361 catacatcgt tcctctctct tcttaccggc aagattaccc gtaccggcat cgattaaaat
      421 ttcaggggtt agcgatatct tcttcgctct cttctttatc aatcgcattt tgtgctttgt
      481 tagccaccac aacaaagggc agataaatta acgttgcgat gccaaggttg aagagtgcga
      541 ccagcaatgc ggcgacgcta ccgttggtgt taaagaaggc tcccagaccg gttggcatgg
      601 tccacggtgc aatattggtc accggaggaa tgatgcccat gtagtacgct gcgagggtga
      661 ttgccgccag aatcggttgt accagtacaa acgggataaa catcaccggg ttcatgataa
      721 ttggcagacc aaacagaatc ggttcgttaa tctggaagat gccggacggc agcgtcagtt
      781 ttgccacctg acgataatca gcacggcgag aggcgataaa gatagccagg atcaggccta
      841 aagtcgcacc actgccccca aggaaaataa aggagtccag catcggcttg gcccagatat
      901 ggaaggtctt accggctgcc agcgccgctt cgacggaacc atattgctga taggtcgcaa
      961 tattttccag cgcccacggc gtcataatgc cgttgtccag tgcggtcagc gccagcgcgc
     1021 catgaatacc gaagaaccag agcagtggaa caaagatcac ataggcccag cccaccacgc
     1081 tacccaacga tgccagtggg gttgagatgg tatccataat gatctgatgg aagttggtgc
     1141 cccaggtatt caacgcccag gcaataatcc ccatcacgga aagaataata aagtcgggaa
     1201 ttaatgccga gaaagaacgc gatactgaag caggtacgct gtcgggcagt ttaatgaccc
     1261 aattgcggcg gacaataaag gtaaacattt ctgccaccac caggccaata ataatcccgg
     1321 agatgatatt cgccccacct aaccagtttg cgccaaccgc ataggcctca ccgacactat
     1381 atggggtgac ggtcataaat gctgcaacgg ataacaaccc agccgccagc gcatcgcgct
     1441 ttacgctctt ctgccagcgc catgccaata aagaacggtg ccattaaaga cattattcct
     1501 aatgttccgt tatatacgtt gccgccaata cctttcagac cattaagtgt ttcaatggtt
     1561 gaggcgtcga ggcgaatacc taaggaataa aaaaacgacc cctccccaaa gcttagaaaa
     1621 acgttgttaa ttaatacaaa catggccccc gcaagggtta acggcattaa gcgaataaag
     1681 ccatttttga ttgcattaac gtgtggctgc tttcctattt taactgcaaa agggaggagt
     1741 accttttcaa gcgatgcaat aacattactc atagaaaaat acccttaaaa accgcaattt
     1801 aaatattgcg gttttgattt atgaaataac tctttgacgg gaaaatttaa aataaattaa
     1861 tttgctgcgg cttttttaat cgctgcaacc gcagccttaa gcacgcctaa accatcgact
     1921 tt
//
LOCUS       DAJTYG010000240         1882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTYG010000240 DAJTYG010000000
VERSION     DAJTYG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.242"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            21..1544
                     /locus_tag="OO568_004099"
     CDS             21..1544
                     /locus_tag="OO568_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6643183.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          497 a          411 c          418 g          556 t
ORIGIN      
        1 cctttggccg ggacaagcaa atgcgcaata cactcatacc catcctcgtt gcgatatgct
       61 tgtttattac cggtgtcgct attttgaaca tccagctctg gtattccgca aaagcagagt
      121 acctggcggg agcaagatat gccgccaaca atatcaatca tatacttgaa gaagcgtcac
      181 aggcgactca aacagcggtt aacattgccg ggaaggaatg cgacctcgag gagcaatatc
      241 agcttggcac tgaagcagct ctgaaacctc acctgcgcac aatcatcatt ctcaaacagg
      301 gaatagtctg gtgtacatcc ctgcctggga atcgggtcct gctgtctcgt attcctgttg
      361 tcccggacag taatttactg ttggctccgg caatcgacac cgttaataga ttacctatcc
      421 tgctctatca gagccaattt gcagatacgc gcattttggt tacgataagt gatcaacata
      481 ttcgcggggc gcttaatgta cccttgaaag gggtaaggta tgtattacgc gtggcggatg
      541 acattattgg accaacgggt gatgtgatga cgcttaatgg acattatccc tataccgaga
      601 aggttcactc cacaaaatat catttcacta ttatctttaa cccgccgcca ctctttagct
      661 tctacagact tatcgataaa ggctttggga ttttaatatt tattctgtta atcgcctgcg
      721 ccgctgcctt cctgcttgat agatatttca ataaaagcgc aacgcctgaa gagatcctgc
      781 gacgggcaat aaataatggg gagatcgtcc ctttttacca acctgtggta aatggtcggg
      841 aagggacatt gcggggagtt gaggtgttag cccgctggaa acaacctcac ggtggatata
      901 tttcacccgc ggcatttatt ccacttgctg aaaaatccgg attaatcgtt ccgcttacac
      961 aaagcctgat gaatcaggtt gccagacaga tgaacgctat cgcgagtaaa ctgccggaag
     1021 gttttcatat tggaattaat tttagcgctt cgcatattat ttcgccgacg tttgtcgacg
     1081 agtgtttaaa tttccgtgac agttttaccc gccgcgattt aaaccttgtt ctggaagtca
     1141 ccgagcgtga gccattgaat gttgatgaaa gtctggttca gcggttgaac attctgcatg
     1201 aaaatggttt tgtcatcgcg ctggatgatt tcggtactgg ctactcaggg ctttcttatc
     1261 ttcatgacct gcatattgat tatatcaaaa ttgatcacag tttcgttggc cgcgtaaacg
     1321 cagacccaga atcaacccga attctggatt gtgtattgga tctggcgcgt aaactttcga
     1381 tcagtatcgt cgctgaaggt gtcgaaacga aagaacaact tggctatctg aaccaaaata
     1441 atatcacatt tcagcagggt tattatttct ataaacctgt tacatacatc gacctggtca
     1501 aaattatcct ttctaaaccg aaggtgaagg ttgtggttga gtgaaaattg atcagtaagg
     1561 ccatagtgcg gtgtaattat agacagctaa ttagctcgtt gcctcttgtt actattgttc
     1621 attattttgt ttgctataat tgtttgaaag ttttgacagg attgccatta gtagcatgaa
     1681 caatagtaat aatctggatt atttcactct ctatatcatc ttttccattg catttatgct
     1741 gatcaccctc ctggtcatcc ttattgcaaa acccagtacc gggctgggag aagtgcttgt
     1801 gacgataaat ttgcttaatg cccttgtttg gctggcgatc aatctggtta atcgatttaa
     1861 gagaaagact cgtcagccac ag
//
LOCUS       DAJTYG010000241         1861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTYG010000241 DAJTYG010000000
VERSION     DAJTYG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.243"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..642)
                     /gene="ipaH3"
                     /locus_tag="OO568_004100"
     CDS             complement(<1..642)
                     /gene="ipaH3"
                     /locus_tag="OO568_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR6643184.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRC"
     gene            1384..>1861
                     /locus_tag="OO568_004101"
     CDS             1384..>1861
                     /locus_tag="OO568_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR6643185.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLH"
BASE COUNT          536 a          320 c          440 g          565 t
ORIGIN      
        1 gcaccggaaa aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag
       61 gctttccaga agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag
      121 aaatgtcagc tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag
      181 catggttagc tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag
      241 catggttagt tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag
      301 tgttgacagg tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa
      361 tgatattagg gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg
      421 tagcgaggac agatttaaac gattcagttg aagctcactg aactgattga tgagacattc
      481 tttaagtagg gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt
      541 atcccatgct gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg
      601 ggacaatgaa aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga
      661 caaatggctt tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga
      721 acttcagcac ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg
      781 tttcagttaa ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa
      841 acatcgtact tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc
      901 aaccccaaat cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct
      961 gatggatgtt cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt
     1021 gctacggaat tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc
     1081 ccgaaaattt ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca
     1141 atggtacgtg tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag
     1201 ggttttattg tttgttgacg aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa
     1261 gccagaagca tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga
     1321 gtccagcgtt ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata
     1381 ttgatgaaac agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc
     1441 tggctttctg gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt
     1501 cgttttgatc cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg
     1561 caaagtttac gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat
     1621 catgaacaga tggcgatgga tgcgctggca ttccagcaga tgtctttgtg gttgatgaat
     1681 ggcggcgact ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg
     1741 gaaaaatgtc agcatttgcc ctttattctt gaagtacaca gtcgcaccgg caagcatgtt
     1801 attgctcatg ccgattatcc agatgatgtt tatgaatggc aaaaggacgt tgatttgcat
     1861 c
//
LOCUS       DAJTYG010000242         1793 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTYG010000242 DAJTYG010000000
VERSION     DAJTYG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.245"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(1..275)
                     /locus_tag="OO568_004102"
                     /pseudo
     CDS             complement(1..275)
                     /locus_tag="OO568_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(390..1028)
                     /gene="ospI"
                     /locus_tag="OO568_004103"
     CDS             complement(390..1028)
                     /gene="ospI"
                     /locus_tag="OO568_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR6643186.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1187..1657)
                     /locus_tag="OO568_004104"
                     /pseudo
     CDS             complement(1187..1657)
                     /locus_tag="OO568_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          507 a          449 c          326 g          511 t
ORIGIN      
        1 gccagtctac ccagcaagcg ctgtaactct tttatttgtt ttatagcggc agccagttct
       61 gaggcaggaa cgacatcctc tccggcagca acagcagtga gacttccttc cagatattat
      121 tttttccact taagcaacag gctgggctga ataccatgca aacgcgccac atgggacacc
      181 gtcatgccag gctccattgt ctgctgaata atggcaattt tttcctgagg agttttacgt
      241 ttacgaagct ccagccccag caaagttcca gtcatctcaa aattagaact agtgttagac
      301 atatatccaa gcctatctct tattttaaga tacatccact gtctggcgtt tcaaggggcc
      361 ggctcaaatc tatgtacagg ctctcagcat cagcaaagcc tcttactttt ccaactacta
      421 ctgttagcat agtgatcaaa ttcctgatca gatacatgaa ttacctgaac cgaactattt
      481 tcatctacaa atgcatctgg cgatttttct acaggatcat aaagattaaa tccctttcct
      541 gtttgaacat ccaaaaacat aattttttca ccatcgttaa atccagctat ccaatgagtg
      601 gcctgttcaa tagaaatcat tactacactt ccttttgaag tattttgtaa aatattttct
      661 gcaatacgct cacgaaattt actataagtt tcattaggat gtgacactat gccttcacta
      721 tcaatttgct ctaaatgcct atgaaaaaat ccggtcatat actctgatat ttcattaggt
      781 acgggagcct ctcttactcc ataccctaac atagccatac aagcatgaag cgcacaccct
      841 gaacagttac catccccacc agacggattt atcattttct gtaaagtcgc tgccaattca
      901 gaatctttat ccatatcacc aagactttta gagtttaaag attccagcaa ttttttaaca
      961 tttacatttc cctcctctgt atgtgcctca tatccagcag ttccctgtaa cgacacccca
     1021 ttaatcatat tgtatcctga cagtatatat actcactata ttttcttcat aacaaatatc
     1081 cctctgatat gacaacaaaa aacggagccg gattcctgtt tttgtgaact gcatctattc
     1141 gtgtctaaaa agtgatccac ttaccaccaa cggcctaata tatacagaac ggagtaaaca
     1201 gggctaacag ttcccgacag gtctcatcat tccgtggccc gaaagtgtaa gccaacacac
     1261 cgccagtttt agtgttgtac gcataccaga gccagtgttg ttgcagcctt gctgccaacg
     1321 aagcgccact gctcatcaag ttcacagata agggcaacat cagcgtgagc gactggcgca
     1381 gatgttatcc gccggggcgg gagcttttta atgtccgtat gacggtattg atgccgattt
     1441 tcagtgtcct ggcggtatca cggccccctg cgccgttatg tgccatttca acgatctgct
     1501 ccttaacgcc gggttttctg gcttcataag agtaagtgag ctggaaaaca cgtttacatg
     1561 agcgacaacg gaaacgctca tgccgggaac aactacggcc atgtcgataa acttcatcgg
     1621 aatgacagcg cggacaatga acagtaacgg tagccatgga aaaccttaaa taccagtaga
     1681 ttaccagaaa cttctgtcga ttgaagacat cacccgaagt ttgaattcat tcgaatactt
     1741 tttacgaggt tcttttcgcc agtcctgtaa ttcgtaagcg ccccatcagc gac
//
LOCUS       DAJTYG010000243         1773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTYG010000243 DAJTYG010000000
VERSION     DAJTYG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.246"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(782..1771)
                     /locus_tag="OO568_004105"
     CDS             complement(782..1771)
                     /locus_tag="OO568_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643187.1"
                     /translation="MNMTKNAFQFGIEPVHITDNDNLQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
BASE COUNT          489 a          427 c          439 g          418 t
ORIGIN      
        1 cggcggtgcc gtaggcaatg cccgttgaaa ttttgtccat ggatttcata gcctcacctc
       61 cgcaaataac ggatggcgta gttttacact gagaaatgaa agggatttga aaagaaaaaa
      121 acgcaaaagc gggcgaaacg atatatacag taaggaaagc actctatcca acaaaccacc
      181 cacagttaat cggaataaaa gcagagtgct tatgaatgat cgcacgcccg aaggttagta
      241 tttctgcaca gcaattttgc aaaaaaaggc gatcattcat aacttaaacg tctttcagtc
      301 actccgggat ttcccatcat cgcagactga aagactctga ctggagcggg cagcgggagc
      361 ctacgacaaa tcgaagacaa ccagctaacc tattgtttct taaagacagg tgaatccagt
      421 tggttgtagt gatagtcaca catttagaga tagaaggtca agacttgaag cacgtaatat
      481 tccctctaaa ggaatagaac tcacggtctg actacccggc ccttttggtc tggctcaggt
      541 caggctatgg gggcttttgg ctcgctcttt atattagatg aggtctcaca tttttcagtc
      601 aaaactcatc gtctaatagc ccatagccag accaccagtc ccagctttag caggaagacc
      661 acgagataga ctttgtgtag gtccatttgg ccctcctact ttctgtattc acgaactaga
      721 ttactagtac aaaataaagg ccagacatcc agttcggaag tctgaccaat agtcgttatt
      781 gttagttagc ttctttagtg aagctcaggc gtttgactac aggcaggaca gcagtagccg
      841 aaccagtacc catcttgatg gtcgtagcag cttcagctac aacaaacacc ttgccgagaa
      901 tctcaaggtc gtgagactgg gagtctccat cacgtgaaag gaccacagta atgctctcgc
      961 catcgtgacg gaagcaaccg aagctcagag ccgtaaaacg cttagccatc atgaacacgc
     1021 cacggtcttc accgttgtaa taggcgacgt cagtccccag catatcgacg atagcgtctt
     1081 caccaccgaa gccctgctta gcagccagac gggagagagc cttgaggtgt gactcatggg
     1141 cgataatgcc gtagtcagaa acctcagaac cgaaagaaga ctccgtgttc tcgtacaggg
     1201 catccatgat agcgtcagcc ttagcggtca ggctaccagt cagggccgga agttctacgg
     1261 tcttcagcag ctttgaggac tccagcacgt caaccatctt agagttaatg gtacgaatca
     1321 cgttatcgca gatgttcagt gcgtcctgct ggagcgtccc ctgagtgaaa ccagtggtcg
     1381 acttctggtc ccaaagcttt gtttggtgag acacgaggat cgcctgttca atcggctcaa
     1441 cggtgatttc agtgccagac ggagcggtca caacttcagg tcgcttacca ccacggacag
     1501 ggaccagcac agagcggtca gccagagtgt gagcaatgat tggagtcggt aagctgttac
     1561 gggtcggcag gacttctaca gggaacggga tgttatcttg agcgtcttta agtacaccgt
     1621 tcagcatagc cttaacagtc ttagctaact ggagggcata gacctgaggg tcggcgttag
     1681 tcggaaggcc ctcattaacc tgaaggttgt cgttgtcggt gatatgaaca ggctcaatgc
     1741 caaactggaa agcgttttta gtcatattca tta
//
LOCUS       DAJTYG010000244         1753 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTYG010000244 DAJTYG010000000
VERSION     DAJTYG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.247"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..264
                     /locus_tag="OO568_004106"
     CDS             <1..264
                     /locus_tag="OO568_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643188.1"
                     /translation="SDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYKTDP
                     EHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            266..870
                     /locus_tag="OO568_004107"
                     /pseudo
     CDS             266..870
                     /locus_tag="OO568_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
     gene            857..>1753
                     /locus_tag="OO568_004108"
     CDS             857..>1753
                     /locus_tag="OO568_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6643189.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLPDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRVF"
BASE COUNT          418 a          398 c          493 g          444 t
ORIGIN      
        1 tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg tacagatggt
       61 ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc cggcatttac
      121 aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga cggttcgtca
      181 tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt gtcggatatc
      241 agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc ttgcgcagta
      301 tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc tttccccgac
      361 agttttatca gtgattttta tggtcttatc tggaacatcg ataccgcaga aaagtatggt
      421 cttgatgtct ggggaaagat tgtgggtgtc agtcgccggc tgacggtaaa ggacgatttt
      481 aattacctgg gcttcagcga gtccaggatg gacaccccgg taatggatga tccctgtccg
      541 tttaatcagg caccgtttta caacggaaaa tcggataccc ggactgttga cctgtctgat
      601 gctgtatacc ggcggctgat actgatgaaa gccatgtcga acattactga ctgttccgtt
      661 ccggatatta acctgatgct gagatttatg ttcggaaaaa aacgccgggc ttatgttctg
      721 aataatggcg ggctgaggat gagttacgtc tttgagtccg cgctctcgtt ggcagaactg
      781 gcgattatcc agtcgtcggg tgcactgcca tccccgccgg gtgtttatgt ttcagtagtt
      841 ttaaaggagt cccgtaatga aggccagtga taaaccccgc cagctggcgg tcccctttgc
      901 gagtaccgga gataaaaacc gtatcccgga caaggcgaca cagcagacca gagagagcgg
      961 taatgctgcg tatgattcag gttttcctcc ggtgaccatg acagcggtct cagcgggagg
     1021 tataccgcca cacggcaagg attttaacgg tctgatgtac gatattaccg cagcaatacg
     1081 gttcgcccag gctggcggtt tgtacacgta taatgccggt tttgcggggg ccattggtgg
     1141 atatgcaaaa ggagccattc tcgccggagt cgcaacaaca gcggtctggc tgaataccac
     1201 ggacgataac ctgaccgatc ctgaaggctc cgacagtgcg ggctgggtaa atcttcttga
     1261 ggatccgaaa aggatattcc tgcggcagaa gaacaatctg ccagaccttc agaataaagg
     1321 gacggcacgg gataatcttc aggtttacag taaagagcag tcagatcaac gctatgttca
     1381 tcgggaaggc gataaaataa ccggagagct gaaaatccgt ggtgttaatg cgctgaggat
     1441 tttcaacgaa gcttttggcc tgatttttcg tcgttcggaa gagtgcctgc accttattcc
     1501 caccagtgaa ggtcaggggg aaaatggcga tattggtccc ctgcgcccgt tcaccattaa
     1561 tttgcggacg ggtgaaatat ccatgtcgca taaagtgtct gttggcggtg gttcgcaggt
     1621 caatggtgcg ctgggtatcg gcgttcagaa cgccctgggg gggaattcaa ttgttcttgg
     1681 tgataatgac accggattta aacagaatgg agacggtatt ctggatgttt atgctaatag
     1741 tcagcgtgta ttc
//
LOCUS       DAJTYG010000245         1745 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTYG010000245 DAJTYG010000000
VERSION     DAJTYG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.248"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(243..797)
                     /locus_tag="OO568_004109"
     CDS             complement(243..797)
                     /locus_tag="OO568_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6643190.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(794..1084)
                     /locus_tag="OO568_004110"
     CDS             complement(794..1084)
                     /locus_tag="OO568_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6643191.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1084..1683)
                     /locus_tag="OO568_004111"
     CDS             complement(1084..1683)
                     /locus_tag="OO568_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6643192.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          418 a          478 c          396 g          453 t
ORIGIN      
        1 ggttttaatt cctatcgatg aactggtggc gaactgtgca gggttggcgt aaccgggtac
       61 ggaaaccggc gagcctttcg gctcccccac acagcccgcc ataaatcgcg aatgtgactg
      121 tgcaaacgat atgaaaaaag acgcgggcgc gtctcatatc gctccgtaaa catccgggac
      181 gccaatcccg acgccagatt ttgctggcgc gtgaggaata tagccccgga taacagattg
      241 agtcaacaaa cggtttttag atccccggaa gagaatgcat cacgcatcgg tagatagagc
      301 atatattcag caagatttag ccatacatct acccgactgc agcacgtccg gaaacaccac
      361 tcagggtgtg cgtcattcag caattcagcc attttacgct tagtcatccc ccgcccttca
      421 taccgttgcc ggaggacact aatcaatcca ggatgctctg ccagcacctc acttatgact
      481 ctatcaatac ataacgcctc tgcatcagta caatgcgcca gccaggtctt ttgcttgcca
      541 ttgatcatct ctcgcaaaaa cgcttccagc tcaggtttct ctattcccgc ttttttcatc
      601 ctgcgcaggg cttcattgac ggctgttttc gtcaattttt tggatgccag caactggtta
      661 aacatatttc ccgtcttacc gccgccaata tacgaccagc gcccccacat gcgcagtttt
      721 ccctgaatcc agacactttc cagcgtggtg agacgaaggt gttccccgct tttgcctgta
      781 tttgttgggt aaatcataaa taaccttcct ttctccagat ttcttgtgtg cgaaaaacac
      841 cttctgcatg catcaggcgt aattcttctt tggtgtaatc gctggttttt acccgcccgt
      901 cgattaaatc gtggcatgag ctacaggcaa tcgctgcctg catatcatgt ggttttgtcg
      961 ctgttccgca cgtccccgcc agcctgtaat gcgccagcac agaagtttcg ggattgtgat
     1021 tgcagtagcc aggaattctg acggtgcaca tctgcccccg cgccgcttta cgtaaatcca
     1081 ccattacgca aactccagta actgcgcggc cacattttcg acttcctccg gagaggaaaa
     1141 tttacggaac aggatccagt tccacagcac attcagtaca gatttataaa cctgctgaaa
     1201 ctcggtttcg tccatgttcg caaatgcgat agattttgcc ctgcgcccac gactaccatc
     1261 aggataaata tgctcggtgt aaaatccggc ctgaatggtt acccactcgc ggaaagcgtc
     1321 aaacgacttt agcaatgccg tatcccgggt tctgcatgtc gcaactgtat taaggtattg
     1381 ctctgcggca tcactcaggg ctggagtgtg ttcccgacct actgattcgc acaggtaatc
     1441 aacgaagcct gataccagtt ttcgttcgcg aggcgtgatc gccccaccga ccggagtcca
     1501 gtaatcaaat cccagttgca ggagtttgaa aaaacgcttg tggaatgcgt agttacgcac
     1561 acgcttaaag tctgcgtgta tccactcacc tattttgatt tgatgcaaaa aatcgcaact
     1621 ctccggcgtc gccgggagaa gtaatccgga agaggtttgt ttgaccagtt gtatatgcgc
     1681 catcgtagtt ctccgctggc gcagtagaat gggtgttcag cccgttatgt agtataccag
     1741 aatta
//
LOCUS       DAJTYG010000246         1726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTYG010000246 DAJTYG010000000
VERSION     DAJTYG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.249"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            278..799
                     /locus_tag="OO568_004112"
     CDS             278..799
                     /locus_tag="OO568_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6643193.1"
                     /translation="MDVYANGQHVFRFQNGVAIAFKNIQAGTARKFTLSSANNSTKNA
                     AFYLWGNPSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRT
                     EGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQ
                     KLINGTWYNVASI"
     gene            802..1347
                     /locus_tag="OO568_004113"
     CDS             802..1347
                     /locus_tag="OO568_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6643194.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            1371..>1726
                     /locus_tag="OO568_004114"
     CDS             1371..>1726
                     /locus_tag="OO568_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6643195.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGA"
BASE COUNT          482 a          361 c          463 g          420 t
ORIGIN      
        1 ggcgaagatt ggatttaaaa atgattcgga tgcagacacc gattcctaca tgtggtttga
       61 aacaggtgac aacggcaatg agtatttcaa atggagacac cgcctcgctg gcggccagct
      121 caaagagctg atgaatctta aatgggattc actaaatatt ctggttaatg ccgtcattaa
      181 tggttgcctt ggcattggta cgacgaatgc gttaggtgga aattcaattg ctttcgggga
      241 taacgatacc ggcctgaaac agaacggcga tggtctgctg gatgtttatg cgaatggaca
      301 gcatgtattt cgttttcaga atggcgtggc tattgctttt aagaatattc aggccggaac
      361 tgccagaaaa ttcacgttat ccagcgccaa caactccacg aaaaatgcag cgttttattt
      421 gtggggtaat ccatccaggc ctgttgttgc agagcttggt gatgattcag gctggcattt
      481 tttcagccag agaaacccgg ataacagcat agtgttcact gttaacggac aggtaattcc
      541 gttaaattac ggaaacttcg atgcccgcta taaatatcga acagaggggg tacaggatgt
      601 acggtatggc catgaaatgt attacagccc cggcagtaac accgtttcgt ggagattttg
      661 cgcaccttcg ggacacgggc tgtcagggat ggcgatatcg gataccggcc gtaactcagc
      721 ggataacgtt gacggtgtgt attaccgacc actgcaaaaa ctgattaatg gcacctggta
      781 taacgtagcg agtatttaac aatgttgcat ttaaaaaata ttactgcggg taatccgaaa
      841 acagcagaac aatatcagat gacaaaacga tattcggtca cctggctttt ttcagaagac
      901 ggaaaaaact ggtatgaaga gctgaagaat ttcgccagcg acacaataaa aatagcttac
      961 accggagatg gtcgcgtggt gtgggtcggt aaggatgtga caggcatcga gccacgcaat
     1021 gccagcgtta tcgaagttcc ggatattacc gctaaccgac ggattaccgc gccgggttac
     1081 tggttttacc gcaatgatga atttgtcttt gactacagac tcaaagcgga agatgagcgt
     1141 gatgcccttc tggctcaggt cagtgcccgg acaggggaat gggaagaaga cctgctgctg
     1201 gggttaatca gcgacgaaga taaagaaaag ctgaaagcct gtcgtattta cgcgaaatcg
     1261 ctgcaggcga tggatttcag caccatcact gataaatcct catacaacgc cattgaatgg
     1321 cctgcctctc cggaaggttc ttcctgattt aatttatcgc gagaaaaaca atgtctgtag
     1381 tgatatcagg tgcgctgact gatggcgcag gtatccccat gtccggatac catattattc
     1441 tgaaatcccg ggtaaacacc ccggaagtgg tgatgaacac tgttgctgat gtgatgacag
     1501 gaaacgatgg tgaatactgt ttccatgcgc ggactggaaa atatggtgtg tatctgaaac
     1561 aggactggcg caacgagtac aacgttggcg acattgctgt atatgaggac tcaaagcccg
     1621 gcacgctgaa tgactttctg attgctcctg atgagggcga cctgaaaccg gatgtcgtca
     1681 aacgctttga ggaaatggtg gcgcaggcgc agcagagcgc cggggc
//
LOCUS       DAJTYG010000247         1717 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTYG010000247 DAJTYG010000000
VERSION     DAJTYG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.250"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..219)
                     /locus_tag="OO568_004115"
     CDS             complement(<1..219)
                     /locus_tag="OO568_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6643196.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNL"
     gene            525..1307
                     /locus_tag="OO568_004116"
     CDS             525..1307
                     /locus_tag="OO568_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR6643197.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(1309..1535)
                     /locus_tag="OO568_004117"
                     /pseudo
     CDS             complement(1309..1535)
                     /locus_tag="OO568_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          481 a          408 c          343 g          485 t
ORIGIN      
        1 aagattacga atagtattat ccgcaaaaat atcatcacct actttagtca gcatcaggac
       61 ttttgaattc aatttagccg ccgccaccgc ttgattagca cctttcccac cacatccgat
      121 tttgaaggca ggtgcttcca gagtttctcc ttctttaggc atctgattag tgtaagtaat
      181 gagatccacc atattggaac caataactgc aatgtccatt tcactacctc ttataaactt
      241 tcgcataaca atggtattta aataacatta gtatgttact tttgcatcat ttgtgactga
      301 gatcgcgatt agcacatcaa cccgatgttt atttaataga cttccagtct catcactcag
      361 gccaacacta tctaatcata agcaacctaa caagattagt gcccaaaatt cagcagccta
      421 tacccttttc atttcaaagg ggcggtcgta tagtatggta atgaaaacaa tgtttactaa
      481 cgccaaaatg ttatttttat aacattctta cggagagaga gttgatggaa acgaagcaaa
      541 aagagcgtat ccgacgtttg atggaactgc ttaagaaaac cgacagaatc catttgaaag
      601 acgcagcgcg aatgctggaa gtttctgtaa tgactattcg tcgcgatctc catcaggaag
      661 atgaacctct gccactgacc ctactgggtg gctatattgt aatggtgaat aaacccgcgc
      721 catccatgcc agtaatccat gacgttccaa aaaatcatcg tgatgactta cctattgcaa
      781 ttctggctgc cggaatggtt aatgaaaatg atctgatctt ctttgataat ggccaggaga
      841 taccactcgt tataagcatg atcccggatg caatcacctt caccggcatc tgttactcac
      901 atcgcgtctt tgttgcgttg aatgaaaagc ctaatgtaac agcaatactt tgtggtggta
      961 cgtatcgtgc cagaagtgat gctttttacg atgccagtaa ctcttcgcca ttagactctc
     1021 tcaatccgcg aaaaatattt atttccgcca gcggtgtgca taatcacttt ggcgtcagct
     1081 ggtttaaccc tgaagatctt gccactaagc gtaaagcgat gaaccgtgga ctacggaaaa
     1141 ttttgctcgc ccgccacgcg ttgttcgatg aagtggcctc tgccagcctc gcaccgatct
     1201 ctgcatttga cgttctgatt agcgatcgtc cgttaccggc agattatgtt acgcactgcc
     1261 agaatggttc tgtaaagatc attacacctg attcagaaga cgaatgactt actgaaaaaa
     1321 caccacaatc ttgttaaaca tcgtcgggtt ggactgatta cgttgcactt tcaccacata
     1381 ttccagctta tctatttggc ttatcaccta ctccagacgc tggtcatcct tgaccagtag
     1441 ccagatatgg cttttgtcgc agtcctgaat cggcaggcag agaatgtctt caacgttaag
     1501 agcgcgacgg gcaaaagccc acaaaggtga gtcattacac ccggatggta aacaggcact
     1561 tccttgttgt agaagccgtt atattgtcca taatgtctgg gtttttaatg catactgggt
     1621 aacaaccgga cgaatcttcg aatatcactg gcgctgccag ccggtttgaa ttcatctcac
     1681 aaccctgcat agagcgaatc tcctgcctgc acgtcac
//
LOCUS       DAJTYG010000248         1669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTYG010000248 DAJTYG010000000
VERSION     DAJTYG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.251"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(72..539)
                     /locus_tag="OO568_004118"
     CDS             complement(72..539)
                     /locus_tag="OO568_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6643198.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(605..844)
                     /locus_tag="OO568_004119"
     CDS             complement(605..844)
                     /locus_tag="OO568_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR6643199.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(979..1584)
                     /locus_tag="OO568_004120"
     CDS             complement(979..1584)
                     /locus_tag="OO568_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6643200.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          537 a          369 c          292 g          471 t
ORIGIN      
        1 agacctactg ctatcacaat tactgagaga aaaaaaagca tccgatgatt catttatgct
       61 gtcctaaaaa attaacaaaa caatgggaaa acaggatatg taagcagaaa agccaatgaa
      121 attggtacag cataaggcac accgttttca cgaattgatt ttcgaaaaaa aagaagacct
      181 gccagaacaa ccagagcgcc gcaaaaggtc attgcgacga gaaaattagc cagttcatgt
      241 tcatcaatag ctaacgaaag aacagttaac agcttgacat caccgccgcc aattatccct
      301 gcggaaaaaa gaaagaagcc cacgagcaaa acgctcccgg ctgcaaccag attgggtttt
      361 tgatgctgta aaatgagtgc caaaaaaata agcacggtta acaccagtaa ataacgatgg
      421 ctaataattc tgagtaatat atctccccat gaaataaaaa taagaaagag cgcaacaaat
      481 aaaagatcga ctcttagtaa ccatattgct gccattgaaa gactccatgt accatacaag
      541 gttgctgcca gacatattcc tttacgtctg acagcctcta gagagttttt ttatttttta
      601 taagttacgg attactcgtt ccggaaatcg ttacctgttg aattgctgca gcgatagcgt
      661 cgaatgcatc tttaattgcg cccaggaagc ctgagttctg atcgccaaag atgaaagcaa
      721 gcagagttgc catagcaaca ccaatcaatg catattcgat tgcagtaaca ccacgttcat
      781 ccttcgcgaa acgcgcaaag cactgtttag ttttaccata gtacttagta ataaaagtat
      841 tcatgttttc ctcgtatatt attatttaaa ttcaaaccac cagcttcatc tgcgtgtcac
      901 cggtatccca tatttttggg atgtggctat aattatccat tcccgctatt tagtcttcaa
      961 aaaaaattac atttaatatc atttatcttg attaagaaat agtaagacta aaaaaacaag
     1021 cgtcgtcagc cagcacactg ctaacacggt aagaacgcta ataaaaacac tccactgctt
     1081 cgttctatac cattcaatat ttttgtcagt atgttttatt gggagcagct cttcagtaga
     1141 gaattcctgg ctgttatcat tctcaggaat gtttgcatca gtgtttactt ccctggcctc
     1201 gctatcgttg gtatagctac tactgtcttc atcactgtca cgctcattct cacagacatg
     1261 tttctcgacc tttcccagat atctgacgcc ttttcgcgga atggttttaa ttagagaact
     1321 cgacaaacct gcgacgtcta atgcctttct aagcatgtaa atctgcccat aataactgct
     1381 gtcgcttacc gaacctttct gctcccgcca aacctcattg ataatatgtt gcttatcgga
     1441 ttctccctgt agtaaaagga gtaaaaatcg atagccgttt tcagacagat tgattacatt
     1501 gcctgtactg gtggtgatac gtcgagacgc tgggtaaaac ttaactccct gccctaaaga
     1561 aaaataaaaa cggtcatcgc tcataagtac cgaaccctgc tttcttcttc acatgactac
     1621 tcattacaac tttgtatgaa gtaagatgca aaatcatatt gtttacatt
//
LOCUS       DAJTYG010000249         1662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTYG010000249 DAJTYG010000000
VERSION     DAJTYG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.252"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(1..933)
                     /locus_tag="OO568_004121"
                     /pseudo
     CDS             complement(1..933)
                     /locus_tag="OO568_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
     gene            complement(961..>1662)
                     /locus_tag="OO568_004122"
     CDS             complement(961..>1662)
                     /locus_tag="OO568_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6643201.1"
                     /translation="ESWGINTTYVEKVPCTSSGVAPIFVNANSSNSILIIKGANKFLS
                     PEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVLLNPAPALRELDMSYA
                     CKGDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNIIVTMSEKGALWMTRDQ
                     EVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAALFAAFSVTGKGMQSS
                     YPSIEQFNEFLTLNE"
BASE COUNT          519 a          366 c          328 g          449 t
ORIGIN      
        1 tcataaggat gttggctata aacttgccaa taaagaagca ggcaaagctg tagaccatga
       61 agtttgaagc atcacgttcg ttgatatcgc ccaactccag tgccagacgg atggtaaatg
      121 accatactgc gacctgcata cccacataaa ggaattgagc cacaatacca cgacgaaagc
      181 gcgaattttt agccagatag cgcagcgtat ccattgctga cgggcgttta tggtgacttg
      241 tctgtgccac tttacaggtt ggaaagcggg ttaaaaggaa caacaccatg accacaacca
      301 gaatcataat catatactta tacggttcaa gggtgttctc taacatcagc accttaaagt
      361 tgtgaatttg ctcggcgttc attccggaca tctgcttctc aaggctttcc ccctcggaga
      421 aaaccagata tttgcccaat aaaatacctg acgcagcacc aatcggataa aaggtctggc
      481 tgatattgag ccgcaatgtg gcataggctt ttggaccgat cattgaactg tatgtgttcg
      541 ctgcagtttc aaggaaactc aggccaatcg caatcgcaaa aatagctgca aggaacatag
      601 tgtaggttgc catatgcgag gcagggaaaa aagtgtacaa ccaccaatat acagcgtcag
      661 gccaattaaa attgccacct tataactggt ctttttaatc acaagggatg ctggtattgc
      721 aattaaaaaa taacctccat aaaatgcgct ctgcaccaat gctgaagcaa agttacttag
      781 cgaaaataca cttttgaatt gagtgattaa tatgtcattt aatgcagctg cgcatcccca
      841 tagcgggaat aaacacgata acaaaataaa ctggaacaag ggagtcttat tcagataccc
      901 atcaggcatc tgaatgatgt ttttatcgtt catagtgcta cctttaactg tgcaggatga
      961 ttattcgttt aaggttaaaa attcattaaa ttgttcaata ctcggataag atgattgcat
     1021 acctttccct gtgacgctga aagcggcaaa gagagcggct tttttcaaag cggcttcaac
     1081 atcaccgctt tgaacataat aatgggaaaa gcaaccaata aatgcgtcac cagcgccact
     1141 agtatcaaca gcatttactt tgaatgcagg aacatggact tcctgatcgc gggtcatcca
     1201 taatgcgcct ttttcgctca tggtaacaat aatattgttc agccctttat caactaacga
     1261 acgtgcggcc aaacgaatat gatcataagt atcaaccgac ataccggtta atatttccag
     1321 ttctgtttca ttcgggataa agaaatcacc tttgcaggca taagacatat ctaactcacg
     1381 caatgccgga gccggattta ataacacttc aataccattt ttcttaccaa actcaatcgc
     1441 gtggtaaact gtttccagtt gaacttccag ttgtaaaacg atcaatttgc attttttcag
     1501 atcttctgca gctcgatcga tatcttccgg ggaaagaaat ttattcgctc ccttaattat
     1561 taatatacta ttgctcgagt tggcattaac aaagatcggt gcaacaccac tgctggtaca
     1621 ggggaccttc tcaacataag tggtattaat tccccatgat tc
//
LOCUS       DAJTYG010000250         1656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTYG010000250 DAJTYG010000000
VERSION     DAJTYG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.253"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..1656
                     /gene="cyoB"
                     /locus_tag="OO568_004123"
                     /pseudo
     CDS             1..1656
                     /gene="cyoB"
                     /locus_tag="OO568_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
BASE COUNT          308 a          440 c          443 g          465 t
ORIGIN      
        1 tatcatgtat atcatcgtgg cgattgtgat gttgctgcgt ggttttgctg acgccattat
       61 gatgcgtagc cagcaggctc ttgcctcggc gggtgaagcg ggcttcctgc cacctcacca
      121 ctacgatcag atcttcaccg cgcacggcgt gattatgatc ttcttcgtgg cgatgccttt
      181 cgttatcggt ctgatgaacc tggtggttcc gctgcagatc ggcgcgcgtg acgttgcgtt
      241 cccgttcctc aacaacttaa gcttctggtt tactgttgtt ggcgtgattc tggttaacgt
      301 ttctctcggc gtgggcgaat ttgcgcagac cggctggctg gcctatccac cgctatcggg
      361 aatagagtac agtccgggag tcggtgttga ttactggata tggagtctcc agctatccgg
      421 tataggtacg acgcttaccg gtatcaactt cttcgttacc attctgaaga tgcgcgcacc
      481 gggcatgacc atgttcaaga tgccagtatt tacctaggca tcactgtgca cgaacgtact
      541 gattattgct tccttcccaa ttctgacggt taccgtcgcg ttgttgaccc tggatcgcta
      601 tctgggcacc catttcttta ccaacgatat gggtggcaac atgatgatgt acatcaacct
      661 gatttgggcc tggggccacc cggaagttta catcctgatc ctgcctgttt tcggtgtgtt
      721 ctccgaaatt gcggcaacct tctcgcgtaa acgtctgttt ggttatacct cgctggtatg
      781 ggcaaccgtc tgcatcaccg tgctgtcgtt catcgtttgg ctgcaccact tctttacgat
      841 gggggcgggc gcgaacgtaa acgccttctt tggtatcacc acaatgatta tcgccatccc
      901 gaccggggtg aagatcttca actggctgtt caccatgtat cagggccgca tcgtgttcca
      961 ttctgcgatg ctgtggacta tcggttttat cgtcaccttc tcggtgggcg gtatgactgg
     1021 cgtgctgctg gcagtaccgg gcgcggactt cgttctgcat aacagcctgt tcctgattgc
     1081 gcacttccat aacgtgatca tcggcggcgt ggtcttcggc tgcttcgcag ggatgaccta
     1141 ctggtggcct aaagcgttcg gtttcaaact gaacgaaacc tggggtaaac gcgcgttctg
     1201 gttctggatc atcggcttct tcgttgcctt tatgccgctg tatgcgctgg gcttcatggg
     1261 catgacccgt cgtttgagcc agcagattga cccgcagttc cacaccatgc tgatgattgc
     1321 agccagcggt gcagtactga ttgcgctggg tattctctgc ctcgttattc agatgtacgt
     1381 ttctattcgc gaccgcgacc agaaccgtga cctgactggc gacccgtggg gtggccgtac
     1441 gctggagtgg gcaacctctt ccccgcctcc gttctataac tttgccgtag tgccgcacgt
     1501 tcacgaacgt gatgcattct gggaaatgaa agagaaaggc gaagcgtata aaaagcctga
     1561 ccactatgaa gaaattcata tgccgaaaaa cagcggtgca ggtatcgtca ttgcagcttt
     1621 ctccaccatc ttcggtttcg ccatgatctg gcatat
//
LOCUS       DAJTYG010000251         1646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTYG010000251 DAJTYG010000000
VERSION     DAJTYG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.254"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..326)
                     /locus_tag="OO568_004124"
     CDS             complement(<1..326)
                     /locus_tag="OO568_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6643202.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPLSLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPD"
     gene            complement(715..981)
                     /gene="ospE1"
                     /locus_tag="OO568_004125"
     CDS             complement(715..981)
                     /gene="ospE1"
                     /locus_tag="OO568_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HCR6643203.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(1090..1220)
                     /locus_tag="OO568_004126"
                     /pseudo
     CDS             complement(1090..1220)
                     /locus_tag="OO568_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1218..1313)
                     /locus_tag="OO568_004127"
                     /pseudo
     CDS             complement(1218..1313)
                     /locus_tag="OO568_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          473 a          277 c          345 g          551 t
ORIGIN      
        1 aaatcaggga gatgtgttaa taggtttttt cttatttcca gtgttgttat ctgaggcggt
       61 aaatctggta atgtttttaa atctaactca gataaattta attctgcctc ttgattttgt
      121 aaacatattt tcagcctatt gaaggcgata ttgcgttgct ctcctggggt accgttcttt
      181 tcccattcgc tccaggcttt taaataaaat tcattacaag atggagtcag ttccataggt
      241 aaagataatg gagttaacga gtatatattg tttatttgat gcataatgcc agaaccgatt
      301 gcctgtatat tggttgattt aatcattata accagaattc ttatcaacga taagaattct
      361 agtgaaatag aatatggctt attaatttta aaacagtttg ctttgtaccc tttctccggg
      421 tattgctttc cccctaagcc gtctatggga aggagtctgc tgttattcct gagaatggta
      481 attcagccat aaaaaggtat ttaaggtcat tgcgtctctc cctatactcg aataatggtg
      541 aagaaagcat gatgaatata tcacgataac gttcagtacg tatcgaggat agatggagtt
      601 actcctctct gaaatagaat tactgctcta tttttttctg ttttcagaag ttattagtaa
      661 caaaccctga tatatttttt tgaaaatgga gcttaaacga gtctgaaatt aacttcagaa
      721 atcagaatac cgttggcttt gtttttcatc aattccggct tcatttatct ttgtcaacca
      781 ttgcatttct gtagcagtga atttcaacgt tttcgtccgg ctggcagcag gtatttgtaa
      841 ttcgtatata gctgcttttt tattgaaaac agtatatcca tctgtagtaa cagtagaaga
      901 taataataca ggatttgata cttcaaggat gatattttct tgtttttgtg gaaggcaagg
      961 gaatatagtt tgtgtaagca tattacttcc ttatgttata ttttggtaaa aaatttattt
     1021 ttttacatta tagtcggcta aatgaagttt actgtaaaaa aacgatactt tttcgggtgg
     1081 cgcttccaat cagtagaata tgtgtttttc aatgaaggag ccgatgactt ttcgtggatt
     1141 tccacggaac gcgagaagca gattgtttta cgtgccagcc gcttgatgcg ggtgcggagg
     1201 gtcaggttgt tacgttcaag atatagcatg tgctggatga agatcccttt tctggtcacc
     1261 tgtttatctt tcgtggccga cggggtgaca ctgttaaaat gtaatgaacg catcccacac
     1321 aattaaacac ggagtagtat gatggttaaa agggtgaaac tggcaggtgt ggagcctcgc
     1381 agctttccgg catcatactg cccaatacgg gatgcataaa gcgtcctcag actggagact
     1441 ccacaaatga aatatacacc ggttggcgtt gatatggcaa aacatgtcat tcagattcac
     1501 ttcatcaatg agcacatgtc agcgccagtg atataagacg gtaatgggga tgcggacgat
     1561 ttcctggact ctcagaagat gaaacatgtt tactgaagaa caaaaaattc gcgctattga
     1621 gctttatttc aaatatggta agaagc
//
LOCUS       DAJTYG010000252         1490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTYG010000252 DAJTYG010000000
VERSION     DAJTYG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.255"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..127
                     /locus_tag="OO568_004128"
     CDS             <1..127
                     /locus_tag="OO568_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6643204.1"
                     /translation="VAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            188..1490
                     /locus_tag="OO568_004129"
                     /pseudo
     CDS             188..1490
                     /locus_tag="OO568_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
BASE COUNT          338 a          368 c          488 g          296 t
ORIGIN      
        1 ggttgcccag ggcaatgttc tgcctgttct gtacggtgaa atgcgcgtgg ggtcgcgggt
       61 ggtatctcag gagatcagca cggcagacga aggggatggt ggtcaggttg tggtgattgg
      121 tcgctgatgc aaaatgtttc atgtgaaacc gcctgcgggc ggttttgtcg tttatggagc
      181 gtgaggaatg ggtaaaggca gcagtaaggg gcataccccg cgcgaagcga aggacaacct
      241 gaaatccacg cagttgctga gtgtgattga tgccatcagc gaagggccgg ttgacggtcc
      301 ggtggatgga ttaaaaagcg tgctgctgaa cggtacgccg gtcctggaca gcgaggggaa
      361 gacaaacttt tccggtgtta cggtggtgtt ccgcgccggc gagcaggagc agacaccgcc
      421 ggaggggttt gaatcttccg gctcagagac tgtgctgggt acggaagtga aatacgacac
      481 gccgatcacc cggaccatca cgtcggcaaa cattgatcgt ctgcgcctga ccttcggtgt
      541 gcaggcactg gtggaaacca cctcaaaggg ggaccggaat ccgtcggaag tccgcctgct
      601 ggttcagata cagcgtaacg gtggctgggt gacggaaaaa gacatcacca ttaagggcaa
      661 aaccacttcg cagtatctgg cctcggtggt ggtgggtaac ctgccgccgc gcccgtttaa
      721 tatccggatg cgtaggatga caccggacag caccacagac cagctgcaga acaaaacgct
      781 ctggtcgtcg tacaccgaaa tcatcgatgt gaaacagtgc tacccgaaca cggcgctggt
      841 cggcgtgcag gtggattcag agcagttcgg taaccagcag gtgagtcgca attatcatct
      901 tcgcgggcgc attctgcagg tgccgtcgaa ctataacccg cagacgcggc aatacagcgg
      961 tatctgggac ggaacgctta agccagcata cagcaacaac atggcctggt gtctgtggga
     1021 catgctcact catccgagct aaggcatggg gaaacgtcgt ggtgcggcag atgtggacaa
     1081 atgggcgctg tatgtcatcg gccagtactg cgaccagtca gtgccggacg gatttggcgg
     1141 cacggagccg cgcatcacct gtaacgctta cctgaccaca cagcgtaagg cgtgggatgt
     1201 tctcagtgat ttctgctcgg cgatgcgctg tatgccggta tggaacgggc agacgctgac
     1261 gttcgtgcag gaccgaccgt cggataaagt gtggacctat aaccgcagta atgtggtgat
     1321 gccggatgat ggcgcgccgt tccgctacag cttcagcgcc ctgaaggacc gccataatgt
     1381 cgttgaggtg aactggattg atccggacaa cggtcatgag acggcgacag agcttgtgga
     1441 ggacacgcag gccattgtcc gttacggtcg taacgtcacg aagatggatg
//
LOCUS       DAJTYG010000253         1488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTYG010000253 DAJTYG010000000
VERSION     DAJTYG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.256"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..452
                     /locus_tag="OO568_004130"
     CDS             <1..452
                     /locus_tag="OO568_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6643205.1"
                     /translation="VGWNWNSGVYDANISGASTLILHFNKNTGSCPAVQFRVNYKNGG
                     IFYRSARDGYGFEAGWSEFYTTTRKPSARDVGAYTQAECNSRFITGIRLGGLSSVRTW
                     NGPGWSDRSGYVVTGSVNSNRDELIDTTQARPIQYCINGRWYNAGSI"
     gene            455..988
                     /locus_tag="OO568_004131"
     CDS             455..988
                     /locus_tag="OO568_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6643206.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQVENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1017..>1488)
                     /locus_tag="OO568_004132"
     CDS             complement(1017..>1488)
                     /locus_tag="OO568_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6643207.1"
                     /translation="LTKNFDVIWLWTEDGKNWYGEVKNFQPDTIKIVYDENNIIVAIT
                     KDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVVKRIYTADEQQQQAESQKAA
                     LLSEAESVIQPLERSVRLNMATDEECARLESWERYSVLVSRVDTAKPEWPQKPE"
BASE COUNT          412 a          325 c          352 g          399 t
ORIGIN      
        1 ctgttggctg gaactggaat agcggtgttt atgatgcaaa tatcagcggt gcatcaacat
       61 taatcctcca cttcaataag aatacgggga gctgccctgc tgtacagttc cgtgtgaatt
      121 ataagaacgg cggtattttt tatcgttcag cgcgtgatgg ttatggattt gaggctggct
      181 ggtcagagtt ttacaccaca acacgcaagc catcagcgag agatgttggc gcatatacgc
      241 aggcagaatg taactcaagg tttattacag gtattcgcct tggtggtctg tcatctgttc
      301 ggacatggaa tggtcccggc tggtctgaca ggtcaggtta tgttgttacc ggttccgtta
      361 actcaaacag agatgaacta attgatacaa ctcaggcaag gccaattcag tattgcatta
      421 atgggaggtg gtataacgcg gggagtattt aacgatgatg cacttaaaaa acatttctgc
      481 tggtaatcct gaaacaaaag agcaatacca gctaacgaaa caatttaaca tcaaatggct
      541 ttatacagag gatgggaaaa actggtatga ggaacaaaag aactttcagc ctgatacgtt
      601 gaaaatggtc tatgaccaca acggcgttat tatttgtatt gaaaaggatg tttcagcaat
      661 taatccggaa ggcgcaagcg tcgttgaggt tcctgatatt acagcaaatc gccgggctga
      721 tatttcaggt aaatggatgt tcaaagatgg catagtgata aagcgaactt ataccgggga
      781 agagcagagg caacaagtgg aaaatgaaaa gcaaagcctg ttgcaacttg tcagggataa
      841 aacccagcta tgggactcac agctacgact gggcatcatt tccgccggga ataagcagaa
      901 attaaccgag tggatgctct acgcgcagaa ggtcgaatcc acagacacct ccagtctgcc
      961 agtaacgttt cccgaacaac cagaatgaga gaaggcccgc tatcgggcct taatttttac
     1021 tctggttttt gtggccattc tggctttgcc gtatccacac ggctgaccag aacactgtag
     1081 cgttcccatg actccagtcg tgcgcattcc tcatctgttg ccatattcag cctgacagag
     1141 cgctccagcg gctggatgac tgattcagct tcggaaagca atgcggcttt ttgtgattcg
     1201 gcctgttgct gctgttcgtc tgccgtataa atccgcttaa ccacagctcc gtccttaaac
     1261 atccacttcc cggaatcatc ggcacgacga tttgctgtta tatcggggac ctcaacgacg
     1321 ctaaagcctt caggattaag cgtggaggca tctttagtga tggcgacaat aatattattt
     1381 tcatcgtaaa caatctttat tgtatctggc tgaaagttct tcacttcccc ataccagttt
     1441 tttccgtctt ctgtccacaa ccagattacg tcaaaattct ttgttaat
//
LOCUS       DAJTYG010000254         1486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTYG010000254 DAJTYG010000000
VERSION     DAJTYG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.257"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..716
                     /locus_tag="OO568_004133"
     CDS             <1..716
                     /locus_tag="OO568_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6643208.1"
                     /translation="LLAKGIFHVELEGVPGSFLLENLGYTFGFIIVIMARQQLFTENT
                     VTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEYMPIFNEETRDAFVKI
                     GMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIILMTWLIALGDTTHIVV
                     GSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMSHAQIRNDMSNKRKAE
                     ARQKAERAENIKKNDKNPA"
     gene            792..866
                     /locus_tag="OO568_004134"
     tRNA            792..866
                     /locus_tag="OO568_004134"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:825..827,aa:Arg,seq:cct)
     gene            1028..>1486
                     /gene="intS"
                     /locus_tag="OO568_004135"
     CDS             1028..>1486
                     /gene="intS"
                     /locus_tag="OO568_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR6643209.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
BASE COUNT          406 a          340 c          367 g          373 t
ORIGIN      
        1 cgttactggc aaaagggata tttcatgtcg aactggaagg tgtgccgggc agcttcttgc
       61 tggagaatct cggttatacc tttggtttta ttatcgtcat aatggcccgc cagcaattat
      121 ttaccgaaaa caccgtgact gcggtactac ccgtcatgca aaaaccgaca atgagcaacg
      181 ttggcttact tatgcggtta tggggagtcg tgctgctggg taatattctc gggactggta
      241 ttgctgcgtg ggcatttgaa tatatgccta tcttcaatga agaaactcgc gatgcatttg
      301 tcaaaatcgg catggatgtg atgaagaaca cccccagcga gatgtttgcc aacgcgatca
      361 tttccggctg gctgatcgcc actatggtat ggatgtttcc tgcagcaggt gcggcaaaga
      421 ttgtggtgat tatattgatg acctggctta ttgccctggg tgacaccacc catatcgtgg
      481 tcggttctgt tgaaatcctc tatctggtgt ttaacggtac gctgcactgg agcgatttca
      541 tctggccctt cgcactacct actttagcgg ggaacatctg cggcggcacc tttatcttcg
      601 cgttaatgag tcatgcacag attcgtaacg acatgagcaa caagcgtaaa gcagaagcac
      661 gccaaaaagc agaacgtgcg gaaaacatta agaaaaatga taaaaaccca gcataaatgg
      721 cgagggttta agcaatcgag cggcagcgta cttaccccgc agtccattag cgggtatact
      781 catgccgcat tgtcctctta gttaaatgga tataacgagc ccctcctaag ggctaattgc
      841 aggttcgatt cctgcagggg acaccattta tcagttcgct cccatccgta ccagtccgca
      901 aaatcccctg aatatcaagc cttccgtata ttcacagttc gtcatggttc gcgtcagatc
      961 gttgacagcc gcactccatg acgggtaaaa agtggataaa ataattttac ccaccggatt
     1021 tttacccatg ctcaccgtta agcagattga agcagcaaag ccgaaagaaa aaccataccg
     1081 ccttctcgat ggtaatggcc tgtaccttta tgtccctgta tccgggaaaa aggtatggca
     1141 gcttcgctac aagattgacg gtaaggagaa aatcctgacc gtcggaaaat atccgcttat
     1201 gactttgcag gaggcaaggg ataaagcatg gactgcgagg aaagacatct cggctggcat
     1261 cgatccggta aaggcgaaaa aggcttcgtc taacaacaat tcatttagtg cgatttacaa
     1321 ggaatggtac gaacacaaga agcaagtctg gtcagtaggg tatgcgactg aacttgcaaa
     1381 aatgtttgat gacgacattt tacccatcat cggcggcctt gagattcagg atattgagcc
     1441 gatgcaactg ctggaagtaa tccgcagatt tgaagatcgc ggtgca
//
LOCUS       DAJTYG010000255         1442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTYG010000255 DAJTYG010000000
VERSION     DAJTYG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.258"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..>1442)
                     /locus_tag="OO568_004136"
     CDS             complement(<1..>1442)
                     /locus_tag="OO568_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6643210.1"
                     /translation="EGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKELGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPES"
BASE COUNT          374 a          379 c          307 g          382 t
ORIGIN      
        1 actttctggt gtcgaaagtg aataattgaa gatgcatttt gttgaggcgt tttctcccca
       61 ggataacgta acctgtcctt tttctggtag accagaaaga taaagttcgc cacggtcacc
      121 gacaattcct gaatctgcca aacttgcatc ttgtatcgca gcaatggctc caaaaggtac
      181 cggtttacca tttgcattta acactctgaa gagaacgcga taacccacat gagtcacaaa
      241 ctccgcttta accactgcac cacgcgtggg aaccatcgta acgacggtat tatcaagttc
      301 tatattcgac ttcgagaaat agttatcgct gattgctacc cgattttcat cataagggtt
      361 tagctgtgtc ttgaccgtat agccgcgcca gtccgtcgca gcaccgcgca tattatctat
      421 ttctaacccg gcggccccag gtgctttaac aagagcaata gtttcaccta actcctgact
      481 taacgttacc ccttcgctgt gtgccagaat tccccctctg ataccataat tcagcctctg
      541 ggaattttgg ctgtagttat agctgccgtt caccgtgcca taagagccat cataggtcag
      601 ggacatatta ccgccaacct catcatcatg actgttatag gactgcatta aattccatga
      661 aaggttattt ctttccagcg ccgtaccgct gagtccgagt tgatgtgagc taccggaatt
      721 atcgttttgc gaaacagaat aggtggcgta actattaccc caccagcccg ataaaggaac
      781 agataaagag agcatgtaga tctgctcact ggtgttatca ctggcatggt tgttccccgt
      841 cccctgtctg gcagtattgg aataactcca ggagacgttc cacgagagac gcttccagct
      901 actgctgtag ccaaattgca ttaatcgttc gacatcatcg gtatgccagt attgttgccg
      961 gcttaatgtc aggtataaat tgccataatc tctccccaac gactgcgtta gcgttccctc
     1021 cacgcgacta cgtcggttac ctgtttccca aaagtcttca gggctgttgc gccgcgatgc
     1081 ccactcattg agggtatagt agccctcggt ggagtaacga tagccaacca ggcgcaagct
     1141 agtgtctgtg tcgtcaaata gttttgaata gagaaagcga tatgactgac ccgtttctgt
     1201 atcatcatgg ctaaaattcg cacgagcatg tgtcacatcg aaagaaattg cgcccaaagc
     1261 gccaagctct ttaccgacac ccagagctgc agaaccataa ttttcagcag cctgcatacc
     1321 tccatataaa gtgatcccgt gggaaaaacc atgaagtatt tgcgcctgaa ggacgtccgg
     1381 tgtaaatccg tcttcatcgc gcaattcacc cacgctgaca tcaacatctg tcagaccttc
     1441 ac
//
LOCUS       DAJTYG010000256         1433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTYG010000256 DAJTYG010000000
VERSION     DAJTYG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.259"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..395
                     /locus_tag="OO568_004137"
     CDS             <1..395
                     /locus_tag="OO568_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR6643211.1"
                     /translation="EKDGFTQPVVVSEEKNHYLVVDGFHRQLLGREADTGKRLRGWLP
                     VACINPERKGQAARIAATIRHNRARGKHQITLMSDIVRDLSRLGWTNERIGTELGMDQ
                     DEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            623..1023
                     /locus_tag="OO568_004138"
                     /pseudo
     CDS             623..1023
                     /locus_tag="OO568_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1034..1405
                     /locus_tag="OO568_004139"
                     /pseudo
     CDS             1034..1405
                     /locus_tag="OO568_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          435 a          235 c          331 g          432 t
ORIGIN      
        1 tggagaaaga tggtttcaca caaccggtgg tggtgtcaga ggagaagaat cattatctgg
       61 tggtggatgg ttttcatcgt cagttactgg ggcgggaagc agatacagga aaacgcctga
      121 ggggctggtt gcctgtagcc tgtattaatc cggagagaaa gggacaggcc gcacgtattg
      181 ccgcgaccat tcgacataac cgggccaggg ggaaacacca gatcacttta atgtctgata
      241 ttgtcaggga tctttcacgt ctgggctgga cgaatgaacg tattggtaca gagctgggaa
      301 tggaccagga tgaagtgctg aggctgaaac agattagcgg gctgacagag ttgtttcagg
      361 aagaggattt cagctcggct tggacggttc gttgatgttg ttaaatatgg gcggtatttc
      421 tgaaagttga tactttgggt agaaaaaatg ataccgaaaa ctatggagga tgtgcttgtc
      481 aggtgactgg acaccatagg tcttaggctt tgataacggg ataaaatgag cggcttttct
      541 cataccatca ggtgtgatga tggcgcagat ttacaaaatc tgattgattc taaagatgta
      601 taagtaaaaa tcaagctttg atatgaaaaa acagatattt attaataata aacctcctgt
      661 agtcccttac tcagggacgc acgcgaaaat atttaaatac attgaaatcc ctctgccgtt
      721 tttctacttt atctatacat caggcgaacc attccacata tctgtccaaa atactgttat
      781 ttatgtatca aaatacaatg gtatatttat taataaatta gttccttttt ctcttttatt
      841 tgacagggat atatctgttc ttcaaagaag agatatttgt gttgtgcgat ttacgtcaga
      901 ggaaataagt gaacataacg ttttatttga tcatgacatt gaacgattaa aaaaatttcc
      961 aaagctcagc ttatatctcc cgattatgtt ttgattgatt tttctagtgg gggggggaga
     1021 tgaaccctat gcaatgtcct ggttaatatc tcagtgtgct catcagtgca ctgataataa
     1081 aaagacggag acagatgcca tttacgataa agttcggagt tcttatttgc tttcatgtat
     1141 tctgaaaaaa aacaaaaatg ttgggcttat tcttcatgca ccatcatttg tttctgtgtc
     1201 tgaaaaaatc gccagaattg tgatggcaaa ttactcccgt aactggagta attccgagtt
     1261 ggccagtgcg gttttaatga gtgaatcttc tttaaaaaga agaatgtaca aagaggtcgg
     1321 ttctataagt acatttgtac ataaaataaa gcttacagaa gctatcagga aattacgaag
     1381 gacaaatact cccatttcgg taatgctgcc aacttactga tttagtgtat gat
//
LOCUS       DAJTYG010000257         1390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTYG010000257 DAJTYG010000000
VERSION     DAJTYG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.260"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..823)
                     /gene="ipaH4.5"
                     /locus_tag="OO568_004140"
     CDS             complement(<1..823)
                     /gene="ipaH4.5"
                     /locus_tag="OO568_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR6643212.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPD"
BASE COUNT          383 a          272 c          304 g          431 t
ORIGIN      
        1 agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac aatggattcc
       61 ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt aatgaagtta
      121 gtaggttatt ggaaacatcc agtgccaata gttcttgcgg taatctggat aatctgcgta
      181 gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt agttcctgta
      241 gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc aagacagtaa
      301 ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt aacgtctcca
      361 gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc agacatgaca
      421 aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc gggagtatat
      481 tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca ggtagggaga
      541 ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt ggtaaagaac
      601 gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat attttcaata
      661 atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag tcatcccaga
      721 tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata catgataagc
      781 cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa cccctcacta
      841 ttcatatctt agtctattaa tgcaaataaa acgagaaagt ctttaaaaaa acaatcctag
      901 agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc cagtaaccgt
      961 cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat ccagtttgga
     1021 ttgatcttta tcccctatat tttcaaccaa tttttatcat ttaactcatt actgtctcga
     1081 tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt gcacgccgta
     1141 aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt aatcgggtca
     1201 cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga ttatgaatgg
     1261 tgcagtcgtg agccgttttc agacaatcgc agggccagta cctcgtcagt caactgacgg
     1321 taaatctgct gttcagtctc acgcatcacc tgtgcaccgg cttccctctc cgcatccgca
     1381 tcaccgctca
//
LOCUS       DAJTYG010000258         1390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTYG010000258 DAJTYG010000000
VERSION     DAJTYG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.261"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(1..573)
                     /locus_tag="OO568_004141"
                     /pseudo
     CDS             complement(1..573)
                     /locus_tag="OO568_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(621..1115)
                     /locus_tag="OO568_004142"
     CDS             complement(621..1115)
                     /locus_tag="OO568_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6643213.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTGQAIAATGV
                     GIRIFNQSDNSQVKLYNDSDYTAIDAEGKAEMKFIARYVATNATVTAGTANADSQFTV
                     EYKK"
BASE COUNT          423 a          281 c          263 g          423 t
ORIGIN      
        1 catcacactg atatgaattg ggttgggatt ctctgctatc agatttcgcc cttcccggcg
       61 ccatttcact ttcggcgcag catcttcggg aataaggcgg atatttgctg gtcgccagaa
      121 cagcttgata cgcgttttta ctgctatttc cagcgagttc gtcgctttgt tgctcgctga
      181 tggcggaata tttttaatat tcagccagaa gactgactcg cgatcatcag gcaatgacac
      241 acctttggtg tgaataatgc gtaatatttt ttcttgcgaa ggttctaatc gtgatactgg
      301 cggaataacg gtaaacggcg ctttgctttt atcctcttta ttaaatggat ctacccaagc
      361 ctggatgaga taaggtattt tttcttcttt gctaaataca gaaatagatt tatcagcatt
      421 attacttaag taaaccaccc gcgtactacc aatgacaacg cctgcataag ttaatgctgg
      481 ttggcctaaa aaaacgctga gtataatcgc ggtaaataac ttacattaaa ctgttgtttt
      541 aaagagcctg attaaacaac tttttctgga cataacgact ccctggccaa agccaattga
      601 ataaaaaggc ccttgataaa ttatttctta tattcgacag taaattgtga atcagcgttc
      661 gccgtaccag ccgttacggt cgcattggtt gccacatagc gtgcaataaa cttcatttca
      721 gccttgcctt cagcatcgat agcggtataa tcagagtcgt tataaagttt aacctgcgaa
      781 ttatcggact ggttaaaaat acgaatacca acgccggttg ccgcaatcgc ttgtcctgta
      841 ccatcaaaac gaacggctgc ttgagtgtac gttgccgggc aattttcaag tttaatactg
      901 aacttctgtg gcgacgccag actacctaca cctgaaaatg catttttact gactttaccc
      961 agtgggacaa tttgatcggc tgtatcggag gtaacttcac aagtagtgtc gacaatttca
     1021 ccgacgaagt taacaacacc atcttctgcc agtgcatgcc cggccagcat tagagatgcc
     1081 agagtgaatg caccgcctat aatattacgc ttcataaaaa ctccattgat ataatatatc
     1141 ctttaccatg aatcatcact tatattaata agtgtatttt tgattcaaag gaatagtaca
     1201 ccaaagagac ggctttatct aaaaataata ttaattacgc aaaaataaaa tatagattta
     1261 ttgtttaaat attatttaaa cagtaaaaaa tcaagtttgt tatcagacat taatgataat
     1321 tcagaatata tttatataga aagatagatg aatttattga tgtcagttat aaaaaagggc
     1381 caccggtcgg
//
LOCUS       DAJTYG010000259         1345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTYG010000259 DAJTYG010000000
VERSION     DAJTYG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.262"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            193..462
                     /locus_tag="OO568_004143"
     CDS             193..462
                     /locus_tag="OO568_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643214.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            459..685
                     /locus_tag="OO568_004144"
                     /pseudo
     CDS             459..685
                     /locus_tag="OO568_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            714..1304
                     /gene="ospG"
                     /locus_tag="OO568_004145"
     CDS             714..1304
                     /gene="ospG"
                     /locus_tag="OO568_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR6643215.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          384 a          262 c          330 g          369 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgaccaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac gggttggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaatctct tgtgattttt aaatatcgta acaaaggtat gacatgaaaa
      721 taacatctac cattattcaa acaccttttc catttgagaa taataattct catgctggca
      781 tagtaacgga gcccattctc ggtaagttaa taggtcaggg gtcgacagca gaaatctttg
      841 aagatgtgaa tgattcatct gctttgtata aaaagtatga tcttattggc aaccagtaca
      901 atgagattct ggaaatggct tggcaagaat ctgagctttt taatgctttt tatggcgatg
      961 aagcatccgt tgttatacag tatggcggag atgtgtacct ccgaatgctg cgcgtgcctg
     1021 ggactcccct tagtgacatt gatacagctg atatccctga taatatagag agcctttatc
     1081 tacagttgat atgtaaattg aatgagttga gtataatcca ttacgatctt aatacaggta
     1141 atatgctgta tgataaagaa agtgaaagtt tattcccaat agattttcgc aatatttatg
     1201 ctgaatatta cgctgcaacc aaaaaagata aagagattat cgaccgacga ttacaaatgc
     1261 gtacaaatga tttttattcg ttattaaaca ggaaatattt atagccgtat ttgttgatgc
     1321 tataagccag aggctcttct atgag
//
LOCUS       DAJTYG010000260         1344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTYG010000260 DAJTYG010000000
VERSION     DAJTYG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.263"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            442..1191
                     /locus_tag="OO568_004146"
     CDS             442..1191
                     /locus_tag="OO568_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR6643216.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          376 a          274 c          278 g          416 t
ORIGIN      
        1 agtggtggat tagcccggca ggtgggcgct ggctcctgag caccataaac agggctttac
       61 ccgtaaatat tggctccttc tgatgccatt ctatttcagg aaggagtgtc cgttaaactc
      121 aggctacctc aaaaagttat cggtgcgttt atcgaaaaac atatgttcta ctaattggag
      181 tcattgtctg taaaagcaac tcaaaaatac aagttgctta ttccccttgt tggtaagaac
      241 ttaattctaa ctgtttgttc tggatgtttt aaacgtcctc ctttttatgc ttgttcataa
      301 cggatattta ataggtaacc tttgttttag catcttctga tttatttaaa ttatgtgata
      361 gcgcacacaa ttttattatc cagtacttta tactcttaat aaaataatta taaatattta
      421 tcctctataa ggagatgtta tatgaaaaga cagcttttta ctcttagtat tgtcggggta
      481 ttttctttaa atacctttgc atcattccct ccgggaaatg atgtgacaac aaaacctgac
      541 ctttactacc tgacaaacga taatgctatt gacagtctgg cattattacc gcctccacca
      601 caaatcggaa gtattgcctt cctgaatgac caggccatgt atgaaaaggg gcgtttattg
      661 cgaaatactg aaagagggaa gctggcagct gaggatgcca atctcagtag tggtggcgtg
      721 gcaaatgtat tttctgctgc ttttggttct ccgataacgg ctaaagattc accagaattg
      781 cataaattac tgacaaatat gattgaggat gccggagacc tggcaacgcg ttcagcaaaa
      841 gaatattata tgcgtattcg accttttgct ttttatggtg tttctacgtg taatacaaaa
      901 gaacaggata cattatccag aaatggctct tatccatcag gtcatacatc gattggttgg
      961 gcaacagcac ttgtattgtc cgaaataaat ccggcacgcc aggataccat tctgaaacgg
     1021 ggttatgaac tgggggacag cagggttatt tgcggttatc actggcaaag tgatgttgac
     1081 gcagcacgta ttgttggctc tgctattgta gcaacactgc actcaaatcc tgtgttccag
     1141 gcacaattac aaaaagcgaa agatgaattc gctaacaatc agaaaaaata atgcttttag
     1201 ccagtacgcc ttataacccc gaccttcagg gctaatgcca gtcagttaag caactgactg
     1261 gctctttttc agggctgtgg ggtatttcca gggcctctcc tttaccactc tcgggaaggc
     1321 cctttccctt cttgtcggta attt
//
LOCUS       DAJTYG010000261         1307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTYG010000261 DAJTYG010000000
VERSION     DAJTYG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.264"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..372)
                     /locus_tag="OO568_004147"
     CDS             complement(<1..372)
                     /locus_tag="OO568_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6643217.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLVTVTRQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKIN"
     gene            complement(372..587)
                     /locus_tag="OO568_004148"
     CDS             complement(372..587)
                     /locus_tag="OO568_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6643218.1"
                     /translation="MYQMEKISTGIAYGTSGGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          367 a          335 c          275 g          330 t
ORIGIN      
        1 gtttattttg cgaagaagcg tcgatgttct gaaattgcca gcgcccacgt tgtaaacgaa
       61 cgagtaaaga gcgccgcgcg ttgtttccgg tatatcgact ttgatgtacg ggttaatttg
      121 tctggtgaca gtgacaaggt ctttattcag gagagctttg cattctgctt cggtatacgt
      181 tttaccaggc atgatgtctt ttccggtgtg tccgtgacat acagtccata caccaatgat
      241 atctttgtat ggtatgtagc tgacaccttc cagaccatcg ttaccacctg ggccagtgat
      301 taacacagat gctatagcaa cagccccgcc accaatagca actgcaacag ccttgcgtaa
      361 tgatggcgac attattcacc tctcgcagcc ttacgcttat cttctcttat tttgaaatac
      421 agattcgtca gataagtcag aagccccaga agcagacttc ccagcacacc aatcgcagcc
      481 cactgtgatg gactgacctg atccaaccac tgcaaaaacc agtagccggc actgccgccg
      541 gaggtgccgt aggcaatgcc tgttgatatt ttttccatct gatacatatc ccgccccccc
      601 ccgacagacc tgtgctatcg gaaagaaaaa aggccatcag caaaactctg atggcctgaa
      661 tcacctttac caatattgta tgaaaaaaca cgcacgacta attgacaata attttcattt
      721 ccattaaaaa agatcgcgta gcattcttaa ttcatgagga actttacccg ccagcaatct
      781 gagtagcgaa agctgttcat ccccaatgtt tttgctggcg ggtccttttt tctacggtcc
      841 tcctccccgg aggggggcat ttcattattt ttctaatcgt tactggtgag aaggcggaag
      901 aaaaagccag ttcttcgccc ctaaataaca gcatgattcc agtccatgca aaatctgatt
      961 tccagctcac acataccgag cgctgtacaa aaaaccgcca gccaaggcag ttaagagtgt
     1021 gtccccgggg tttgcttaga atattttgta agttgtccga agagatattt acaacaccag
     1081 aatgatgaat catcagtccc ctgccagagg ttcactacgt aacctgaccg acaatgcacc
     1141 attctggtgc tgaaaaaaga gcactggaac tgcaacaaaa caacatcaca gaacaggaga
     1201 tggtaaggag tcagacattc ccacacaata ttgtgtcagt gcaaataaca accttcgtct
     1261 caaatctcgc tggagcgggc agcgggaatc gaacccgcat catcagc
//
LOCUS       DAJTYG010000262         1299 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTYG010000262 DAJTYG010000000
VERSION     DAJTYG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.265"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            82..1275
                     /locus_tag="OO568_004149"
     CDS             82..1275
                     /locus_tag="OO568_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR6643219.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
BASE COUNT          387 a          273 c          298 g          341 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta attttgtggg gatccctca
//
LOCUS       DAJTYG010000263         1256 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTYG010000263 DAJTYG010000000
VERSION     DAJTYG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.266"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            102..620
                     /locus_tag="OO568_004150"
                     /pseudo
     CDS             102..620
                     /locus_tag="OO568_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            987..1256
                     /locus_tag="OO568_004151"
                     /pseudo
     CDS             987..1256
                     /locus_tag="OO568_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          390 a          279 c          253 g          334 t
ORIGIN      
        1 ctctgaccac aatctgccgg ttacgccgaa tctgctgaat cagaacttta cgcccacagc
       61 acctaatcag gtctgggtcg cagattcagt tgtccaggcc ttccgaaatc aaccgactga
      121 aggcgctggt cgcgaaactg cagcgtatgc agttcggtaa aagctcagaa aaacttcgcg
      181 caaaaaccga acggcggata caggaagcac aggagcgaat cagcgcactt caggaggaaa
      241 tggcggaaac actgggtgag caatatgacc cggtactgcc atcttccctg cgccagtctt
      301 cagcccgtaa accgttacca gcctcacttc cccgtgcccc ccgggtcatc cggccggaag
      361 aggaatgctg tccggcctgt ggtggtgaac tcagtccact gggatgtgat gtgtcagagc
      421 agctggagct tatcagcagc gcctttaagg ttatcgaaaa acaacgccct aaactggcct
      481 gtcgccggtg cgaccatatc gtgcaggcac cggtgccttc aaaacccatt gcacgcagtt
      541 atgccggagc ggggcttctg gcccatgttg ttaccggaaa gtatgcagac catctgccat
      601 tataccgcca aagtgatcta ctattccaca cagctattta gctctctttt tgttgagctc
      661 ccatgattct ttcatttatt gttactaatt attttatgtt atcatgatgt tagaacacgc
      721 tctgctctgt gtctctattt tatctatcat attctgagga ttaactacat aacaaggcgc
      781 aagatatata tccaaaaaca aataataccg ttgcaataat tcctttcagt aaaggaggtg
      841 tgattaattt caggggattt cagagtaaaa attgtacaac atattaaaat atgttcattt
      901 cgttataata cacacaagaa aatattaagt tagataatat attctaaata accatagata
      961 aaaacgcaca taatggggaa tattttatga aaatacctga agcagtaaat catattaatg
     1021 ttcagaataa tattgatctt gttgatggaa aaacgaatcc gaataaagca acaaaagcat
     1081 tacaaaaaat atattacgcg taacaaactc atcttcttct ggcataagtg aaaaacattt
     1141 agatcactgt gctaatactg tgaaaaactt tttaaggaag tctatagcag cgcaaagtta
     1201 tagcaaaatg ttttctcaag ggaccagttt caaatcacta aatctttcta ttgaag
//
LOCUS       DAJTYG010000264         1227 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTYG010000264 DAJTYG010000000
VERSION     DAJTYG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.267"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..884
                     /locus_tag="OO568_004152"
     CDS             <1..884
                     /locus_tag="OO568_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6643220.1"
                     /translation="PTISLSAVIYFPPKFDETRQYPAIVVSHPGGGVKEQTAGTYAEK
                     LAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTTLSYVDNTRIGAMG
                     ICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSIDALPHIEAGSNART
                     SDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPGYATLRSLNQIITY
                     DAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGANHMDLYDGKA
                     YVAEAISVLAPFFEETL"
     gene            884..>1227
                     /locus_tag="OO568_004153"
     CDS             884..>1227
                     /locus_tag="OO568_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6643221.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGV"
BASE COUNT          325 a          280 c          317 g          305 t
ORIGIN      
        1 atccaaccat ctctttgtct gcagtgatct atttccctcc aaaatttgat gaaacccgtc
       61 agtatccggc aattgtggta tctcacccag gtgggggcgt taaagagcaa acggccggaa
      121 cctatgccga aaagctggcg gaaaagggat ttgtcactat tgcttatgac gcatcttatc
      181 aaggtgaaag tggcggcgag ccgcgtcagt tagaaaaccc atacattcgt actgaagacg
      241 tcagtgccgt gattgattac ctgacaacgc tttcttatgt cgacaatacc cgaattggtg
      301 cgatgggaat ttgtgccggt gcaggatata cagccaatgc tgcaattcag gatcgccgca
      361 tcaaggccat cggtaccgtt agtgcggtca atattggttc gatgttccgc aatggttggg
      421 aaaacaatgt gaaatctatt gatgctctgc cacacattga agctggctca aacgcccgaa
      481 ccagcgatat tagcagcgaa gaatacgcca ttatgccact ggctccaatg aaggaggccg
      541 atgcgccgaa cgaagaactg cgtcaggcct gggagtatta ccacacacct cgtgcgcagt
      601 atccaacagc accgggttac gctactttgc gcagccttaa ccagattatt acctatgatg
      661 cttaccatat ggcggaagtg tacctgactc agccgatgca gattgtggcg ggcagccagg
      721 cagggagcaa atggatgagt gacgacctgt acgatcgggc atcaagccag gataaacgct
      781 atcacattgt tgaaggcgca aaccatatgg atctttatga cggcaaagcc tatgtggctg
      841 aagcaatttc agtattagct ccgttctttg aggaaactct gtaatgaaac atattcaaat
      901 ccgcaatagt gatatggcct ggcatattgc tgccaatatt cagtttccac caaattttga
      961 tgagtcaaaa cagtatccgg cgattatcag cgtccatcct tttggcagtt gtaaggagca
     1021 gacgtctggc aatatctatg gcaaggcatt ggctgaaaaa ggctatgtcg ttctcgctta
     1081 tgacgccagt ttccagggag aatctggtgg ggagcctcgt tggatagaag atccgacaca
     1141 gcgcgtagag gatattagcc gtgttatcga ttatgcagta actctgccat atgttgatgc
     1201 tgaacgcatt ggcgtgctgg gtgtgtg
//
LOCUS       DAJTYG010000265         1200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTYG010000265 DAJTYG010000000
VERSION     DAJTYG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.268"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(3..743)
                     /gene="cysQ"
                     /locus_tag="OO568_004154"
     CDS             complement(3..743)
                     /gene="cysQ"
                     /locus_tag="OO568_004154"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6643222.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            933..>1200
                     /locus_tag="OO568_004155"
     CDS             933..>1200
                     /locus_tag="OO568_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6643223.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSA"
BASE COUNT          278 a          324 c          295 g          303 t
ORIGIN      
        1 atttagtaaa tggacactct gaaccccgga ttcaggaacg actcgcgcgg agtgtaatcc
       61 agcggtttac cctgccagtc gtgaacgtgc gctccggcag ctgcagccac agcatgtcca
      121 gcggcggtgt cccaaatatt cgttggtccg aagcgcgggt acagctgcgc ctgtccttcc
      181 gccaccaggc agaatttcag cgaagagccg atggacgtgg tctgatgttc gccaagctgt
      241 tgcagatact ctttcagctc cgcatctgca tgggaacggc tgataaccac cagcggcggg
      301 cgcgcatcgc ggacctgaat cagcttgcgc acaccgcact cttctttcca ggccttgcct
      361 tctgccgcgc tgtacattac gttcattacc ggcgcataca ccacacctaa aatcggtttg
      421 ccatggtcaa tgagcgcaat gttaacggtg aattcgccat tacgtttaat aaactcttta
      481 gtaccatcca gcgggtctac cagccagtaa cgctgccagt gctgacggac ttcccaaccg
      541 ggaggatctt cttcagaaag gaccggaata tccggtgcca gcgtacgtaa accgtccatg
      601 ataacggtgt gagcggcaat atccgctgcc gttaccggag aattgtccgc tttgctgacg
      661 acgtccatcg gtttcgtccc gtcgtagacc tgcataatgg catcgcctgc attccgtgca
      721 agctggcata cttgatctaa catttctcca cctcgtctct gtgagcggtg ttaacttatt
      781 gttttactta taccctatcg ttaatgaatg cgccaactgt gatagtgtca tcattttcaa
      841 agcgtaaaat tgtggcattc ttcactgttc tataagtaag gcgtttattc tcccttttct
      901 ttcgtattcc cgatgataaa aggatgtccc tgatgattaa gtttagcgca acgctcctgg
      961 ccacgctgat tgccgccagt gtgaatgcag cgacggtcga tctgcgtatc atggaaacca
     1021 ctgatctgca tagcaacatg atggatttcg attattacaa agacaccgcc acggaaaaat
     1081 tcggactggt acgtacggca agcctgatta acgatgcccg caatgaagtg aaaaacagcg
     1141 tactggtcga taacggcgat ttgattcagg ggagtccgct ggccgattac atgtcggcga
//
LOCUS       DAJTYG010000266         1197 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTYG010000266 DAJTYG010000000
VERSION     DAJTYG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.269"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            14..925
                     /locus_tag="OO568_004156"
     CDS             14..925
                     /locus_tag="OO568_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6643224.1"
                     /translation="MINEGDKSQEDIADEAGMSPATLSALRKGEYNGNVEGMHDKLQK
                     WYQVWQKGQNLSGTPGVVKTQIFCDLHELFKTVRSLGIISVVVGVPGVGKTFAAREYC
                     RRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAIRRKLGGGKETSGR
                     VNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADATRRGTDELARLFSR
                     FAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRVLTHTLNHAWLAAK
                     GAGTSLNKSHIKAAFKEAYSNPRLLNW"
     gene            941..1168
                     /locus_tag="OO568_004157"
     CDS             941..1168
                     /locus_tag="OO568_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643225.1"
                     /translation="MMSRNIKMATDAQNWLQAHGSHVNESYLGVARPVLEITCPPPEL
                     VRNAVRIMEHKSGVARSVWTARLNGCQIIWR"
BASE COUNT          334 a          255 c          334 g          274 t
ORIGIN      
        1 cactattaac gctttgatta acgagggcga caagtctcag gaagatattg ctgatgaagc
       61 cggaatgtct ccggcaacac tctcggctct gcgtaaagga gagtacaacg gtaatgtcga
      121 aggtatgcat gacaaattac agaaatggta tcaggtctgg cagaagggac agaacttgtc
      181 aggtacccct ggtgttgtga agacccagat attttgtgat ttgcatgagc tgtttaaaac
      241 agtgcgctca ctcgggatta tcagtgtcgt tgttggcgta ccgggtgtcg gaaaaacctt
      301 tgctgcccgg gaatattgtc gcaggacagc taatgcctgg atggtgactc ttgctccggc
      361 acattcgagc gtaacggaat gcctccttga aatagcatat gcgctggaaa ttaataatcc
      421 gggaagaaat aaggggaata taacccgcgc cattcgcaga aaactgggtg gtggtaaaga
      481 aaccagtggc cgtgttaacc cccttctgat tattgatgaa gctgatcact taagtgtgga
      541 tggtctggag caattacgcg ccattcagga tgccactggc gtggggatgg ttttaattgg
      601 caacccgaaa cagatggcgg atgccacccg tcgtgggact gatgaactgg cccgtctgtt
      661 cagccgtttt gccatgacaa aacaactgcg caaagtaaaa aagaccgatg tgatggctgt
      721 agccaaagca tggggagtaa cacaggaaga tgagctggac ctgctgatga gggtagccga
      781 aaagcccggg ggattgcgtg tattaactca cacactgaat catgcgtggc tggcggcaaa
      841 aggtgcaggc acatccctga ataaaagtca cattaaagcg gcttttaagg aggcttacag
      901 caaccccagg ttactgaact ggtagtaaag gacataaacc atgatgtcac gaaatataaa
      961 aatggcaacg gatgcgcaga actggttaca ggcgcacggg agtcacgtaa atgaatcata
     1021 tctcggcgtg gcgcgtccgg tacttgaaat cacctgccca ccaccggagc tggtcagaaa
     1081 cgctgtcagg attatggagc ataaatcagg ggttgcccgt tcagtatgga cggcccgcct
     1141 taatggctgc cagattatct ggcgttaata acggcttatt tatttgtgag gaataac
//
LOCUS       DAJTYG010000267         1185 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTYG010000267 DAJTYG010000000
VERSION     DAJTYG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.270"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(1..432)
                     /locus_tag="OO568_004158"
                     /pseudo
     CDS             complement(1..432)
                     /locus_tag="OO568_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(369..1112)
                     /locus_tag="OO568_004159"
                     /pseudo
     CDS             complement(369..1112)
                     /locus_tag="OO568_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          266 a          390 c          296 g          233 t
ORIGIN      
        1 ccgttgtctg tgtacgggga gttctggctt tcggtgccag catctgtgca acaccgccaa
       61 gcgtcatact ggcaccgaga gaaaacagca gattactcgc cataattcct accccggcat
      121 ccatatagca accgccataa cagctgcccc cagcaccgcc tgaaacacac cgccactttt
      181 agctcccacc agacgcggta ctatgtggat cactgcacca tttgccagcg gctcattaag
      241 acgggcagac aattcggttt cacctgcatc acgcccggca atgcgcacct gataccagcc
      301 gtcactcagt ttctgacgaa acgccgggag ctgtgtggcc agtgcgcgga tggcttcagc
      361 ccccgttttc acacgaaggt cgatgcggcg gccaaatcgt tgcaaatccc cgtaaaggca
      421 gatgcgtgcc attcccggtg acgccagagg gagtgtgtgc gtcgctgcca tttgtcggta
      481 tacctctctc gtttactcag ttgttcagga atatggtgca gcagctcacc atcgccgcag
      541 taaatggcgg catgattcgg caccgatgaa ccaaaacagc acagcagcac atcgcccggt
      601 tgtgctgatg acaacggcac ctgatacagc cctgtggcct ccagattatc cagctagaga
      661 ttctgaccgt gacgccacca gtcatccccg cggtgaaaat ccggcatctc aatccccgcc
      721 agatgataag catcccggaa cagcgtgtaa cagtccgtca ccccgtgctc aaagcgccgc
      781 ccggtgagat gcggcacaca gcggaacttg tgaatcgccc cccggcagac cagccaccac
      841 ggcaaatcac tctgcacctg cagccgccgg tcagcctcac tcagccaggg cagaccaccg
      901 ggatgactgt ggaccagtgc cacaatctca ccctgcattt gtgcccgcag ccagtcctcc
      961 ggagccatcc ggaaccgttc ctccggctca ccggagatat tctcgctggg aagatacctg
     1021 tctccctccg gcgttctcac cacgaatcca cacgactccg ctggcgcaca tcgccgggcg
     1081 tgcgccagaa tcgctgattc tgtctgtgtc atggatttac tgagaaagtt tattgatgga
     1141 aaggaaaccg ccgaaattac cggtattgtt gcgcaactca caccc
//
LOCUS       DAJTYG010000268         1124 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTYG010000268 DAJTYG010000000
VERSION     DAJTYG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.271"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(695..1111)
                     /locus_tag="OO568_004160"
     CDS             complement(695..1111)
                     /locus_tag="OO568_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643226.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
BASE COUNT          296 a          281 c          199 g          348 t
ORIGIN      
        1 agatgacgat aagggcgatt aacatcgcct tttgctgctt catagcctgc ttctccttgc
       61 ctttcggcac gtaagaggct aacctacatg tgttcagcat ggattgagcc tcagattaat
      121 gttaagcgtc ttgcaggacg cgtaatgtta actggggctt ttctctatct gccttttggt
      181 gttcatgcct gagacagata gcctcaagca cccgcagtca ttctacttaa ctaagatttc
      241 cctgcaaacc gtttttgtcc ggcacagtaa atatccaact aaaccaatag cgttcgctgt
      301 atttaccgcc agtattcaat gcacatgacc gccatgaaca cccctaaaaa aagggcattt
      361 atatgtccaa acattaatat caaaacatca attttttcca tataccttgc tgtgaagatg
      421 atgggcatac atgatgcgaa caaccagaac gcaacaaaca aaaactgcaa tgcgtttttc
      481 attattcccc ctacaatcaa tgtgcaataa catttaaaca cacctcaatt tggccggaca
      541 tataaatatc taaaccagaa aaaatcactt acatagcgtt acaaactctt tagtctaaat
      601 actcatcgta aaacattccc catacttatc agtccgttcc gcgccaggta gcttattgcc
      661 ttatctggca acctataatc aggtttccgc tttttcagtt ggctggtcgt ttaaccgaca
      721 tagttaaccc attaatctgg ttgccggatg ctggtggatt ttcgcgtttt agttgttcat
      781 aaaagtgcac agctttaacc agttcttctg atgtaaccgg gactggtggg gcagtgaata
      841 aggcctgaat ttcatagttc ggcctgtcgt tacaatcctc ttttttcggt acatatttcc
      901 agtcaccaac ccacaactcc ccctgagagt ccataacacc ttttttcacg tagcgatatc
      961 gccacgctat cggctctgct tccagcgatg cccgtgcaat tttgaataac tcgccctcta
     1021 ctcgcgccat ccctgaattg gggtggcatt tcgtaatcgc tatttttaat ttggcttctt
     1081 cgattaattg ttcttttgtt aattcagtca ttttcattac cgcc
//
LOCUS       DAJTYG010000269         1120 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTYG010000269 DAJTYG010000000
VERSION     DAJTYG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.272"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..>1120
                     /gene="pdeF"
                     /locus_tag="OO568_004161"
     CDS             <1..>1120
                     /gene="pdeF"
                     /locus_tag="OO568_004161"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6643227.1"
                     /translation="TYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVFLFYLP
                     LALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGGYRVFT
                     PRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNLGT
                     LINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFALWLLAL
                     GALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMISIHSY
                     QNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDPVVHLP
                     NVRALNRALRDALWSALCYLRIPGMEMLVKNYGIMLRI"
BASE COUNT          244 a          254 c          251 g          371 t
ORIGIN      
        1 caacttatat aaaaatacgt gataaatggt gggggcttcc gctgttcctg ccttctttaa
       61 tcttgcccat tttcgcccac attaatactt tcgcgcatat ttcttccggt gaggtttttc
      121 tcttttatct gcctttggca ctgatgatca gcatgatgat gtttttcagc tgggcggcat
      181 tgccagggat caccttaggg atttttgtcc gcaaatatgc agagctgggt ttttacgaaa
      241 cgctctcatt aacggctaat tttattatca ttatcattct ctgttggggc ggttacaggg
      301 tctttactcc ccggcgtaac aacgtttcac atggtgatac ccgtttaatt tcccagcgta
      361 tattctggca gatagtgttt cctgcaacgc tgtttctgat acttttccag tttgctgcat
      421 ttgtaggatt actggcgagc agagaaaatc tggtcggtgt catgcctttt aacctcggga
      481 ctttaatcaa ttatcaggcc ttactggtgg gtaatctgat cggtgtcccg ctgtgctact
      541 tcatcattcg ggtagtgcga aatccatttt atttacgtag ctattattcg caattaaaac
      601 agcaggttga tgccaaagtc accaaaaaag agttcgcgct ctggctactg gcattaggtg
      661 ctttattgtt gctgttatgc atgccgttaa atgaaaaaag cacaattttt agcaccaatt
      721 ataccttgtc attattgctg cccctgatga tgtggggagc aatgcgctat ggttataagc
      781 tgatttcgct gctctgggcg gtcgtgttga tgatcagcat ccacagctat caaaattaca
      841 ttcccattta tcctggctat accacgcagc tgaccataac ctcctccagt tatctggtat
      901 tctcttttat tgtcaattat atggctgtac tggcaactcg tcagcgagcg gtagtcagac
      961 gcattcagcg gcttgcgtat gtggacccgg tggttcatct gccaaatgtt cgcgccctga
     1021 atcgcgcgtt gcgtgatgcc ctctggtctg cgctttgtta tttacgcatc cctggcatgg
     1081 aaatgctggt taagaactat ggcatcatgc tgcggattca
//
LOCUS       DAJTYG010000270         1100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTYG010000270 DAJTYG010000000
VERSION     DAJTYG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.273"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..1019
                     /locus_tag="OO568_004162"
                     /pseudo
     CDS             1..1019
                     /locus_tag="OO568_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          312 a          269 c          253 g          266 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgagaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat attccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcatt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc
//
LOCUS       DAJTYG010000271         1087 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTYG010000271 DAJTYG010000000
VERSION     DAJTYG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.274"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..111)
                     /locus_tag="OO568_004163"
     CDS             complement(<1..111)
                     /locus_tag="OO568_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6643228.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWL"
     gene            complement(183..1052)
                     /locus_tag="OO568_004164"
     CDS             complement(183..1052)
                     /locus_tag="OO568_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCR6643229.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLWQDEHLLRLCLR
                     QALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQV
                     HHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGALAVTAK
                     GAMTALPCRQELE"
BASE COUNT          290 a          315 c          229 g          253 t
ORIGIN      
        1 cagccaaata gcgtataacg accaccacag cgaccaggaa ataaaaaaga gaaatgagta
       61 actggatgca aaacgatagt acgcatttct gaatggaata ttcagtgcca taattacctg
      121 cctgtcgtta aaaaattcac gtcctattta gagataagag cgacttcgcc gtttacttct
      181 cactattcca gttcttgtcg acatggcagc gctgtcattg cccctttcgc cgttactgca
      241 agcgctccgc aacgttgagc gagatcgata attcgtcgca tttctctctc atctgtagat
      301 aatcccgtag aggacagacc tgtgagtaac ccggcaacga acgcatctcc cgcccccgtg
      361 ctatcgacac aattcacaga cattccagca aaatggtgaa cttgtcctcg ataacagacc
      421 accacccctt ctgcaccttt agtcaccaac agcatggcga tctcatactc ttttgccagg
      481 gcgcatatat cctgatcgtt ctgtgttttt ccactgataa gtcgccattc ttcttccgag
      541 agcttgacga catccgccag ttgtagcgcc tgccgcaaac acaagcggag caaatgctcg
      601 tcttgccata gatcttcacg aatattagga tcgaagctga caaaacctcc ggcatgccgg
      661 atcgccgtca tcgcagtaaa tgcgctggta cgcgaaggct cggcagacaa cgcaattgaa
      721 cagagatgta accattcgcc atgtcgccag cagggcaagt ctgtcgtctc taaaaaaaga
      781 tcggcactgg ggcggaccat aaacgtaaat gaacgttccc cttgatcgtt cagatcgaca
      841 agcaccgtgg atgtccggtg ccattcatct tgcttcagat acgtgatatc gactccctca
      901 gttagcagcg ttctttgcat taacgcccca aaaggatcat caccgacccg acctataaac
      961 ccacttattc cgcctaatct ggcgattccc accgcaacgt tagctggcgc gccgccagga
     1021 caaggcagta ggcgcccgtc tgattctggc aagagatcta cgaccgcatc ccctaaaacc
     1081 catactt
//
LOCUS       DAJTYG010000272         1079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTYG010000272 DAJTYG010000000
VERSION     DAJTYG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.275"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..724
                     /locus_tag="OO568_004165"
     CDS             <1..724
                     /locus_tag="OO568_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6643230.1"
                     /translation="YLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIIST
                     FAIDRREKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPSSYIEAAKEEGVTI
                     LSALSMLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSR
                     AFNHALSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPW
                     RSIGNLITLIVGILCVSVMFFS"
BASE COUNT          306 a          230 c          198 g          345 t
ORIGIN      
        1 ttaccttgtc ggtagttggc aacctgatct attaacaacc caggtagagt tcaatcagaa
       61 tacccttcac cagatatgga tatcgattcc cgtgatggtt ttcgccttta gccatacgcc
      121 cattatttct acgtttgcca tagacagacg tgaaaaatat ggcgaacacg ctatggataa
      181 atgcaaaaaa attatgaaag tcgcttatct catcatctgc ataagtgtac tgttctttgt
      241 ctttagctgc ctgctttcta ttccatcttc gtatattgaa gcggctaaag aagaaggggt
      301 caccatttta tcggcgcttt ctatgctgcc gaacgcccca gcatggttgt caatttccgg
      361 gattattgtc gcagtagttg cgatgtcgaa atcattcctg ggtacgtact ttggcgttat
      421 tgaaggtgcc acagaggtcg tcaaaacaac actacagcag gttggtgtaa agaaaagtcg
      481 tgcatttaac cacgcactat caattatgtt ggtatcgctg attaccttca ttgtttgttg
      541 cattaacccg aacgcgattt cgatgattta cgcgatcagc ggcccgctca ttgccatgat
      601 acttttcatc atgcctacgc tgtcaacgta tctcatcccg gcgcttaaac cctggcgttc
      661 catcggaaat ctgattacgc tgatcgtggg tatcctgtgc gtatcggtaa tgttctttag
      721 ctaatatccg ttgtggatgg ttcaaaattg ctacaatttt gaaccattcc atttttcata
      781 atattcattt gttattattc cgccctatca cttctctcgt catatcctag aattatcagt
      841 tagttaaaca cacaaagaga tctgaacatc ttaaataaga ctatttaaga tgcataactt
      901 agattcgcaa gatatcttct ggtcattcaa aaacaatttg cgatatagat attctcatac
      961 catgagatag ttatctggac cacgttcgac catgcaagat taaatcagtg aaacataatc
     1021 atatttaata acgagaaatg catttttaaa tatcttaatc agaattatag caaatacag
//
LOCUS       DAJTYG010000273         1018 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTYG010000273 DAJTYG010000000
VERSION     DAJTYG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.276"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO568_004166"
     CDS             complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO568_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6643231.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLT"
BASE COUNT          327 a          149 c          176 g          366 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt tttttgtaat
      601 gcttttgtat ctttgttcgg atttattttt ccatcaacaa gatcaatatt gttctgaaca
      661 ttaatatgat ttactgcttc aggtattttc ataaaacatc ccccattatg tgcgttttta
      721 tctgcagtta tttagaataa tattatctaa cttaatattt tcttgtatgt attataacga
      781 acatatttta atatgttgta cgattttcac tctgaaatcc cctgagttta atcacacttc
      841 ctttactgaa aggaattatt gcaacagtat tatttgtttt tggatatata tcttgcgcct
      901 tgttatgtag ttaatgctca gaatatgata gataaaatag agacgcaaaa cagggcatgc
      961 tcataacatg atgataacat aaaaaattag taacaataaa tgaaagaatc atgggcgc
//
LOCUS       DAJTYG010000274          994 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTYG010000274 DAJTYG010000000
VERSION     DAJTYG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.277"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..783)
                     /locus_tag="OO568_004167"
     CDS             complement(<1..783)
                     /locus_tag="OO568_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6643232.1"
                     /translation="MKPVHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTR"
     gene            875..994
                     /locus_tag="OO568_004168"
                     /pseudo
     CDS             875..994
                     /locus_tag="OO568_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          278 a          165 c          275 g          276 t
ORIGIN      
        1 gcgagtcgac aatggattgt tataaatatt aacatctgca taaatgtgca gattcatgat
       61 actgtctggc aatgtgttca gctgattgtt agggatatat agtgattgca gacgttgtgg
      121 aagttcgggc agtctgtgta gttggttttc cccaacgttt aaaagcctca gattttgagg
      181 aaggggcggt agctcttgta atctgttatc aaaagcactt agttcctgta gttcacatgg
      241 taattctggt aaggcagtaa gctggttgtt gccaacagaa agtttttgta gagccggggg
      301 aagatcaggt agcgtctcca ggctattgag aagggctgag agtgactgca aggaagatgg
      361 aagagaaggc aaacatgtta aaactctgtt aagtgaaaca tcaagagcga ccaggtgagg
      421 cggaagagcc gggagcctgc tcagtctgtt atcgctggct ttaagtacag ttaaggacgg
      481 agggagttct ggcagagagc gtagctcatt gctagagatg ttaagttctt gtatgtgcgg
      541 gggcaggtat gggagagagc gtaatctgag taaacttaaa ttgagggctg gctcttgaaa
      601 agccagacat attttcagta atcgaactgc ctgtgttcgg tcttctgttg cagcaccttc
      661 cttggcccag ttatcccaga tgcggtgata atccgtgata ttttgctctt ctacggataa
      721 acgggatata catcctaaac cacaaaagga gcggaaaaaa gaaggattgt ggacaggttt
      781 catttattat tgatcctcat tgtttatata ttaatatatt cgtgcaaatg gagtgaggat
      841 tctttaaaaa aacgcgcctg taaatagtta agaacaggaa gaactgttgg ggatgtggct
      901 ggccgaaaat gaaggggtga agttctggct caatgtgctg actgaactga aaaaacgcgg
      961 ttggtcgccc taccttttca tcaagccagt agtc
//
LOCUS       DAJTYG010000275          983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTYG010000275 DAJTYG010000000
VERSION     DAJTYG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.278"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO568_004169"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO568_004169"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6643233.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMKALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDHLVSPEGLAPEWD"
BASE COUNT          259 a          219 c          221 g          284 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggt
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctt cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DAJTYG010000276          967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTYG010000276 DAJTYG010000000
VERSION     DAJTYG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.279"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(13..495)
                     /locus_tag="OO568_004170"
     CDS             complement(13..495)
                     /locus_tag="OO568_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6643234.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDNARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(486..788)
                     /locus_tag="OO568_004171"
     CDS             complement(486..788)
                     /locus_tag="OO568_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6643235.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          292 a          237 c          195 g          243 t
ORIGIN      
        1 tcagactcag actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa
       61 accaaaacgc tcataaaatg ctctggcgtt atcatcaatt gcatgtacca taacagcctt
      121 aatgccaacc tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt
      181 accgaaacca tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg
      241 gacaggattg ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc
      301 agtagctaat gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt
      361 gcgagaagca ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac
      421 ctcatttcca cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt
      481 gacatttatt tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc
      541 acaggagctt ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa
      601 cgttgatcca ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta
      661 cggttcttat tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc
      721 cggatgttaa tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa
      781 acctccattg gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct
      841 atacagaatc acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac
      901 attgtgacga tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga
      961 ttttgat
//
LOCUS       DAJTYG010000277          966 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTYG010000277 DAJTYG010000000
VERSION     DAJTYG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.280"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(41..116)
                     /locus_tag="OO568_004172"
     tRNA            complement(41..116)
                     /locus_tag="OO568_004172"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(81..83),aa:Thr,seq:tgt)
     gene            complement(122..195)
                     /locus_tag="OO568_004173"
     tRNA            complement(122..195)
                     /locus_tag="OO568_004173"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(159..161),aa:Arg,seq:tct)
     gene            complement(197..272)
                     /locus_tag="OO568_004174"
     tRNA            complement(197..272)
                     /locus_tag="OO568_004174"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(237..239),aa:Ile,seq:cat)
     gene            complement(449..>966)
                     /locus_tag="OO568_004175"
     CDS             complement(449..>966)
                     /locus_tag="OO568_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6643236.1"
                     /translation="TRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRLEQKA
                     AFSKQSIAKVD"
BASE COUNT          241 a          256 c          207 g          262 t
ORIGIN      
        1 tggaaggctg aggtaatagc cattatacga tgcccgcata tggtgccgac taccggaatc
       61 gaactggtga cctactgatt acaagtcagt tgctctgcct gctgagctaa gtcggcgcag
      121 gcccaccacc gaggacttga acctcggacc gtcaacttag aaggttgatg ctctatccgg
      181 atgagctagt ggtggttggt ggcccttgct ggacttgaac cagcgacctg gcgattatga
      241 gtcgctcgct ctcaccactg agctaaaggg ccggaggcag aataataacc atatgtcatc
      301 acatctgcaa actcatctga ccaccagcac gtttaacgtc ctgtgccatt tttcaggcat
      361 aaaaaaaccg ctcggcggcg ggttttgctt actttaccat cgcgtacaaa atcggcaaag
      421 tatcagattt gcatgaaata tatgcctttt aatctacttt tgcaatactt tgctttgaaa
      481 atgccgcttt ttgttctaaa cgtgttctca tcacaaacaa taaagcctca ctatccagtc
      541 ggtgaaaaat gtgtttcatt acaacccagt gaccagtaaa tgttttggac cagtttttgg
      601 ttgttactcc cgccagtaat gccagctcct gatattcata accttcccca ccaaaaagtt
      661 ctgcttttac cgcctgcgcc gccagccaga ttaatttttt caggcgttcc tgtgttttct
      721 ctgcaatttt cctagtgcca gaatgagcct taaactcttc ccacgcccag tgggttatgg
      781 tgatctgata ctcccagcaa atacttccac tgtaacacca caacaaccag gctttatggt
      841 gctcttcaag agacagaaca gcccgccgcc acgatgatgt cgaaaacgca acctgactga
      901 ccagaggaat tgacgccccc ttcgccagtg attgttttcc cgggattggt ggattatccc
      961 gcgtta
//
LOCUS       DAJTYG010000278          957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTYG010000278 DAJTYG010000000
VERSION     DAJTYG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.281"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..>957
                     /locus_tag="OO568_004176"
     CDS             <1..>957
                     /locus_tag="OO568_004176"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCR6643237.1"
                     /translation="LRNRVENRLWHSLAACIDDSQTQQLLDLLSVPAGSRYSLLDQLR
                     AGPTKVNATSLVQAIGRLQTIRSLGVTLPAITPVSDIRIAAMARYASTAKITALQRLP
                     EKRKLATLVAFSCCMEATAQDDALELLEALLRDLFNEAVQADKRNRQRTLKDLDRAAE
                     ILAKACRMLLDDKLSDTDVRDSIFNIIPEDVLTHAVNNVTSIIRPDNNVYFNELDSKF
                     KTVRRFLPDLLSRIHFEGNASAKTLIEALCWIEVNLKKKKTDNDAPREIINKPWQQHV
                     IRKDGSIDFHAYTFCALKELQLALKKRDIYVNPSWRYADPRA"
BASE COUNT          286 a          212 c          203 g          256 t
ORIGIN      
        1 gattacgcaa cagagttgaa aaccgtctct ggcattcact ggcagcttgc attgatgact
       61 ctcagactca acagcttctg gatcttcttt cggttcctgc cggaagccgc tattcgttac
      121 ttgatcaatt acgcgccgga cctactaaag tgaatgccac atcactggta caggctatag
      181 gtcgccttca gacgatacgt agcctaggag tgacattacc tgcaattacg ccagtttcgg
      241 atatccgtat tgcggccatg gcacgttatg cctcaacggc taagataaca gcgcttcagc
      301 gcttaccaga aaaaagaaaa ctggctacgc ttgttgcatt ctcttgctgc atggaggcga
      361 cagctcagga tgatgctctt gagttacttg aagcgctact ccgggatctg tttaatgaag
      421 cggttcaagc agataaacgt aaccgccaac gaacacttaa agatctggat cgtgcagcag
      481 aaatactggc aaaagcatgt cggatgcttc ttgacgataa attatctgat actgatgttc
      541 gtgatagtat cttcaatatc attcccgaag atgtactgac tcatgcagta aataatgtga
      601 catcaattat tcgaccagat aataacgttt acttcaatga acttgattct aaattcaaaa
      661 cggtgcggcg tttcttacct gacttacttt ccagaataca ttttgagggc aatgcctcag
      721 ccaaaacatt aattgaagcg ctttgctgga ttgaagttaa tttaaaaaag aaaaaaacag
      781 ataacgatgc accacgcgaa ataatcaata aaccgtggca acagcatgtg ataagaaaag
      841 atggtagtat tgatttccac gcctatacat tttgtgcact taaggaactt cagcttgcac
      901 tgaaaaaacg ggatatttat gtcaatccca gctggcgtta tgcagatccc cgggcag
//
LOCUS       DAJTYG010000279          954 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTYG010000279 DAJTYG010000000
VERSION     DAJTYG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.282"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            77..616
                     /locus_tag="OO568_004177"
     CDS             77..616
                     /locus_tag="OO568_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR6643238.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(842..>954)
                     /locus_tag="OO568_004178"
     CDS             complement(842..>954)
                     /locus_tag="OO568_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR6643239.1"
                     /translation="KKLFIDGKRIGDREYAEVLIAITEYHRALLSRLMAD"
BASE COUNT          351 a          200 c          180 g          223 t
ORIGIN      
        1 aacaagtcat cgtcgtttat tccagaaaga cggaatagga ttgagccaaa caggaaaagc
       61 aacacaactt gtcattatgt cgcatggtgg atggaaagag atctcccatc cacaaacact
      121 attcacgcgt caaaaaggcg atggatggac tgtagtcccc aaaaacctaa gaattgattt
      181 ttatacaaaa gacaacgact tcacaaaggg actaagcgtt ctttcagagg taaacaaacg
      241 ccatactgaa gctcaaaaag ggttaacacc atcactaaac ataagcaaag atgacctaaa
      301 agttctagcg aacgcgcgca atatacctca atcaaaatta gaagaagaaa tgatgtcaca
      361 agccatatat agaaaagaat acgcaacaag taatgagaag ataaaaaact atgctctata
      421 ttaccatgaa caagcccaaa atattataca aagacatcaa gatggcttag gaaacaaaaa
      481 tattgatata gcatttataa ctgataaaaa tcataagaag cacctttctg acatatttaa
      541 agtaataaat gaatcaggag taaaatatga tgtaattcac tttggcgctt gccgagtaaa
      601 tagagaggag aaataatcat catttaccat aatcccttaa ttgtacgcac cactgaaacg
      661 cgctgaacgc gattctgacg cagacaccac aaaaatagat gttttacccc ggaaaaacag
      721 gaaggatgcg tcaggatcgt tttcagctcg ctgcatagct atgcatgaaa gcgaatggag
      781 atcactttgg gagcttaccg tgttcatacc gtctgttttc aacagttttc tccccgggaa
      841 gctaatctgc cataagcctg gataacaggg cacggtgata ctccgtaata gcgatcagca
      901 cctccgcata ctccctgtcc ccgatacgct tgccgtcgat aaagagtttt ttct
//
LOCUS       DAJTYG010000280          922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTYG010000280 DAJTYG010000000
VERSION     DAJTYG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.283"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..>922
                     /locus_tag="OO568_004179"
     CDS             <1..>922
                     /locus_tag="OO568_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR6643240.1"
                     /translation="IELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQLDRLRRMLF
                     GQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSPPSENPIAS
                     KPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQLDIINTAFK
                     VIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIPLYRQSEIY
                     ARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTK
                     TGRLWVYVRDDRNAGSSLPAAVWFAYSADRK"
BASE COUNT          252 a          233 c          248 g          189 t
ORIGIN      
        1 attgagctgc ttaaagccat gttgcgtaag caacagagtc ggcttcgaca gtatgcctgt
       61 caggtcgcgg gctatgagca ggaaattgaa cggctgaaag cgcaactcga caggttgcgt
      121 cgtatgttgt tcggccagag ttcagagaaa aagcgtcata agcttgaaaa tcagatccga
      181 caggcagaaa aacgactgtc ggaactggaa aaccggctga acacagccag aaatcttctg
      241 gaagatgcat cgtcagtcac agattcacct gacaccagtc ccccgtcaga aaacccgatc
      301 gccagtaagc ctgaatcccc gggacgaaaa tcttcacgaa aaccgctgcc ggcagaactt
      361 ccccgggaga cacatcgcct tctgcctgct gaaaccagtt gcccggcctg tggaggtgtt
      421 ctgaaagaaa tgggggaaac aatctcagag caactggata tcattaatac cgcctttaaa
      481 gttatcgaaa ccatacgtcc caaactggcc tgtagccggt gtgatgtcat cgttcaggca
      541 ccacttcccc ctaaaccgat cgaacgcggt tatgccagtg cagggttact tgcacggatc
      601 ctggtcagca aatatatgga acatatccct ttatatcgcc agtcagaaat atacgcgcga
      661 cagggcgtgg agctgagccg taataccatg gtgcgctggg tatcagaaat ggcagacaaa
      721 ctccgtcctc tgtatatagc gctgaatgac tatgttctgg aggcaggaaa ggtgcacgca
      781 gatgacactc cggtgaaagt actggccccg gggaacggaa agacgaaaac gggtcgtctg
      841 tgggtatacg tcagggatga tcgtaatgcg ggttcatccc tgccggcagc cgtctggttc
      901 gcgtattcgg cagatcgcaa ag
//
LOCUS       DAJTYG010000281          910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTYG010000281 DAJTYG010000000
VERSION     DAJTYG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.284"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..648
                     /gene="ospC2"
                     /locus_tag="OO568_004180"
     CDS             <1..648
                     /gene="ospC2"
                     /locus_tag="OO568_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6643241.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNAKSDMALYLLSKFNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYK
                     VLEYFINNGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
     gene            complement(797..>910)
                     /locus_tag="OO568_004181"
     CDS             complement(797..>910)
                     /locus_tag="OO568_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR6643242.1"
                     /translation="RLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
BASE COUNT          309 a          138 c          191 g          272 t
ORIGIN      
        1 aaaactgatg tgccaatatt taatacaaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa agattccgag agttttgtta taataaaaac
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagcacaga taacttcaaa aagataagac tcagagatat atctttggag
      241 gatgccatta aagcatccaa ttatgaagaa attaacaata aggtgactga taaaaaaatg
      301 gctcatcagg cccttgctta ttctctaggc aatgcaaaat ctgatatggc actttattta
      361 ctatctaaat ttaattttac aaaacaagat gtcgcagaga tggagaaaat gaacaacaat
      421 atgtattgtg agctgtatga tgttgagtat ttacttagtg aagatagtgc caactataaa
      481 gtgctagaat attttattaa taatggattg gttgatgtaa acaaaagatt tcaaaaagca
      541 aatagtgggg acactatgct tgacaatgca atgaaaagca aagattcaaa aacaattgat
      601 tttttattaa aaaatggagc ggtatcaggc aaacgatttg ggaggtgacc taaaatatat
      661 ttaggttgtg ccctgaatct gacgttgtca tttttgttat attgatcgat ataggggatg
      721 gtgcagaccg ttcccctgca acaacacatt gcatgtggtg aacgccccgg gaatcctgga
      781 gactaggctg tgtcccttaa ttacgtaagc gttgataaaa gagtcggatg cagcccagtt
      841 tcaccatcgc caggtaattt ctcgccgttt tgtcgtaacg cgtggcgatg cggcgatatt
      901 ctttcagccg
//
LOCUS       DAJTYG010000282          893 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTYG010000282 DAJTYG010000000
VERSION     DAJTYG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.285"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(190..369)
                     /locus_tag="OO568_004182"
                     /pseudo
     CDS             complement(190..369)
                     /locus_tag="OO568_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          250 a          234 c          196 g          213 t
ORIGIN      
        1 tcataatttt tctgccggaa atgaagctga aaagcgcgga caagcgcctt atatccgtca
       61 tccgtctcca caccagaaat actgtatcca taggtcttca gccgtgacac gatatcaacc
      121 ttatccggaa tgccctgcga ttcaaaaaat aaatatttag tcagtaattt tatgtttttc
      181 ctgagaaaat tactcttttt cgaactccag tgagcgttca cgactcatca gtgtgaaaat
      241 gatggcaaaa acagcagaca gtatcagtac gataaatgtg gccccccatc ccagataatc
      301 gacaacaagt cccattccca gtgtcgcaac aattgagccg aagatatagg aaaatgtgcc
      361 tgacagccct aacagaataa gtttacgcgt aaatcgcccg gaatccagaa gagcattaag
      421 tatcacataa acaggttcca taccagtcct gaccgcatca ggacgccagt aaataaaatc
      481 tcgtagtcca ccggcatcag agacaagagt aatcgtcgtt accggtattt cgactggttt
      541 gtactggtgt gagcggacaa aactactgta cgtctctcat acacgctcgt aagcggccct
      601 cacggcccgc taacgcggag atacgccccg actgcgggta aacccttgtc gggaccactc
      661 cgaccgcgca cagaggcatt ttcatcgctg acaggccgca tggcctcgca gagccggagg
      721 gggactggtg atattcggac gaccgtaccg gcttgcgccg ggcttcggcg gttttacacc
      781 tgtgctacat gtaaccaccg tatgccgcct tatatgcgct gactcgcatt atttgccttg
      841 ttccgtctga aagtaatcac tatgattaaa tatgattaac aactaatcgg gta
//
LOCUS       DAJTYG010000283          881 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTYG010000283 DAJTYG010000000
VERSION     DAJTYG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.286"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..810
                     /locus_tag="OO568_004183"
     CDS             <1..810
                     /locus_tag="OO568_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6643243.1"
                     /translation="NLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKH
                     GQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGK
                     YWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKTGETATE
                     TLTQRELVARLKQHIPEKFFKMVRYFGFLASRVCGEKLPQVYRALGMDKPEPVAKVCY
                     AQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDISLLRYMPA"
BASE COUNT          215 a          203 c          292 g          171 t
ORIGIN      
        1 aatctgctgt atgccgcccg gaaacggggg caggaacccg gtatcttctg cgccatccac
       61 acgtatggcc gtcgtctcaa ctggcatccg catgtacatg tgtctgtaac ctgtggaggt
      121 ctgaataagc atggtcagtg gaaaaagctg agcttcctga aagacgcgat gcgttcgcgg
      181 tggatgtgga atatgcggca acggcttctg aaagcgtggt cagaggggct ggcgatgccg
      241 gagtcgttgt cacatatcac gacggaatca cagaggagaa gcctggtgct gaaagccgga
      301 ggaaaatact ggcatgtgta catgtcgaaa aaaacggccg gcgggaggaa tacggcgcgc
      361 tacctgggtc gttatctgaa gaagccgccg atagcggcct cccggctggc acattacaac
      421 ggaggggcga gcctgagctt ccgttacctg gaccacaaaa cgggagaaac ggcgacggaa
      481 acgctgacac agcgtgagct ggtcgcgagg ctgaaacagc acatcccgga gaagtttttt
      541 aagatggtga ggtacttcgg gttccttgcc agccgggtgt gtggagagaa gctgccgcag
      601 gtgtaccgtg ctctggggat ggataaaccg gaaccagtgg cgaaagtgtg ctatgcacaa
      661 atggtgaaac agttcctgag tcgtgacccg ttcgaatgcg tgctgtgtgg cggccggatg
      721 gtataccgcc gggccattgc gggactgaat gtggaggggc tgaagaaaaa cgcgcgggat
      781 atcagtctgc tgaggtatat gccggcctga cacaggctaa gtgtgcccgg gaatcggtgc
      841 aagtataaaa aacagcctgt tgcggtataa gaatcgacca t
//
LOCUS       DAJTYG010000284          860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTYG010000284 DAJTYG010000000
VERSION     DAJTYG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.287"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..92
                     /locus_tag="OO568_004184"
     CDS             <1..92
                     /locus_tag="OO568_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HCR6643244.1"
                     /translation="QVVAALMAIGDELRRLRLAVREQGTRDDS"
     gene            82..>860
                     /locus_tag="OO568_004185"
     CDS             82..>860
                     /locus_tag="OO568_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR6643245.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELTPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGR
                     LELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLP
                     DNLPETKQALAESVTRGIDALYHVGEIKGRQDVIQALTEAGIDVVRVTRSSISIADPN
                     GGKNVRLKGAFYEQSFTDGCGVREKAERESRIYRENAERRV"
BASE COUNT          224 a          197 c          284 g          155 t
ORIGIN      
        1 ttcaggtggt ggccgcactg atggccatcg gggacgagct gcgccggctg cgtctggccg
       61 tcagggaaca ggggacgcgg gatgatagtt aagtttcatg cccggggaaa aggtggtggc
      121 agtggtccgg ttgattacct gctgggaagg gagcgtaacc gggaaggggc aacggtgctt
      181 cagggtaatc cggaagaagt ccgggaactc atcgatgcca cgccatttgc gaagaaatac
      241 acgtccggtg ttctgtcgtt cgcggagaag gagctgacgc cgggaggacg tgaaaaagtg
      301 atggcgagct ttgagcgtgt actgatgccc ggtctcgaaa aaaatcagta cagcatcctg
      361 tgggtggagc accaggacaa gggacggctt gagctgaatt ttgtcattcc gaacatggag
      421 ctacagaccg gaaaacgcct ccagccgtac tacgaccgcg cagacaggcc tagaattgat
      481 gcctggcaga cgctggttaa tcaccattac gggctgcatg acccgaacgc cccggagaac
      541 cgcaggacgc tgacactccc tgataatctg ccggaaacga aacaggcgct tgcagagagc
      601 gtcacacgcg ggatagacgc tctttatcat gtcggggaga taaaaggccg tcaggatgtg
      661 attcaggcgc tcacagaggc agggattgac gtggtcaggg tgacgcgaag cagtatcagc
      721 attgcagacc cgaacggcgg gaagaatgtc agactgaaag gagcgtttta tgagcaatct
      781 tttacagacg ggtgcggagt tcgagaaaaa gctgaaagag agagccgaat ctacagagaa
      841 aatgctgaac gacgagttca
//
LOCUS       DAJTYG010000285          853 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTYG010000285 DAJTYG010000000
VERSION     DAJTYG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.288"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..761
                     /locus_tag="OO568_004186"
                     /pseudo
     CDS             1..761
                     /locus_tag="OO568_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          231 a          184 c          203 g          235 t
ORIGIN      
        1 agcgcattct gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa gatacctctt
       61 atgcccgtgt aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact ggttacggtc
      121 agtttgaact agacctggaa gcatcaaaca gacacaaccc tgaccaaact cgtttggcat
      181 atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat gtcggtgtcg
      241 cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcggtgac tcatgggctg
      301 gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt aacacagatt
      361 tctttggcat ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa aatgaaggta
      421 caggcaacta caaagcaaat ggcgatggcc atggtttatc tgcaacttac actattgacg
      481 gtttctcatt tgctggtgct tatgcaaact ctgaccgtac tgattggcag tctggtgatg
      541 gtaaaggcga aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct aacaatgttt
      601 atgctgctgt gatgtacggt gaatctcaca acatgaattc cgacgacggt gatgttgtga
      661 acaaaacaca aaacttcgaa gctgttctgc aatatcagtt cgactttggt ctgcgtccat
      721 ccatcggtta ctcctattct aaagcactgg acgtagcagg ttggatttgc ccctatattt
      781 ccagacacct gttatcactt aacccattac tggcccgctg ccgtagatat tcccgtggcg
      841 agcgataacc cag
//
LOCUS       DAJTYG010000286          782 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTYG010000286 DAJTYG010000000
VERSION     DAJTYG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.289"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..730
                     /locus_tag="OO568_004187"
     CDS             <1..730
                     /locus_tag="OO568_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6643246.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIAWAITTQQKEYQA"
BASE COUNT          225 a          182 c          190 g          185 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctgggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aa
//
LOCUS       DAJTYG010000287          774 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTYG010000287 DAJTYG010000000
VERSION     DAJTYG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.290"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..377)
                     /locus_tag="OO568_004188"
     CDS             complement(<1..377)
                     /locus_tag="OO568_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCR6643247.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKR"
BASE COUNT          239 a          170 c          160 g          205 t
ORIGIN      
        1 tcacgcttat aatcggccgg tgcaatcgta tagccgttta caggcgctat cagcgatgag
       61 cataaaaaat ggttatcacg ggcatagatc aattcattaa tataaagata gccacgaata
      121 atattcaaca ttcgcttttg atgggctgga gtgcaaactt gcccctgata gcgttcagct
      181 gcatcgctca ctaaatctgc ctggcgaatg accagctcgg atttatccag tgccagttga
      241 gcaaaggttc ggagctgatt attcacttct gattttgccc aaaggaaggc aatccatagc
      301 gaaagaatga tgggaaagag aactaaaaag ataatgcccg gcaacgccag taattggtgt
      361 cgtgcacgat gactcattgc tatcaatttc ctgcctgttt atacccggcc attgcaaggt
      421 gaaaccctgg ctaccgacgt ctttagataa atcttacggt ataaatagca cttttgcctg
      481 atttgaaatt gatacctttt taatcgtcac ctggatgaaa ataattcatt gatattcaaa
      541 attaattttc tacaggaata cagaggcggc gggaagatat gcccttaatg ccactgaaaa
      601 accacataat ctgtacagca ataaaagtca cggcctaata tggctaacaa acaatcaatt
      661 gtgatatggt tcacaaaatt aatgaaacaa acagagtgtt tcatttttgt gttctgcaag
      721 ccccgcgata gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg tcca
//
LOCUS       DAJTYG010000288          746 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTYG010000288 DAJTYG010000000
VERSION     DAJTYG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.291"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            104..>746
                     /locus_tag="OO568_004189"
     CDS             104..>746
                     /locus_tag="OO568_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6643248.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMN"
BASE COUNT          263 a          142 c          124 g          217 t
ORIGIN      
        1 aagcaatacc tggagaaaga gtacaaagca aactgtatta aacttaataa gttatattgt
       61 atttcactag agttcttatc gttgataaga aattctggtt ataatgatta aatcaacaaa
      121 tatacaggta atcggttctg gtattatgca tcaaataaac aacatacact cgttaactct
      181 attttctttg cctgtgagtt tgagtccatc ttgtaatgaa tattatttaa aagtctggag
      241 tgaatgggaa aagaacggta ccccaggcga gcaacgcaat atcgccttca ataggctaaa
      301 aatatgtttg caaaatcaag gggcggaatt aaatctatct gagttagatt taaaaacatt
      361 acctgattta ccgcctcaga taacaacact ggaaataaga aaaaacctat taacatatct
      421 ccctgatttc ccaccaatgc ttaaggtaat acatgctcaa tttaatcaac tggaaagctt
      481 acctgcctta cccgagacgt tagaagagct taatgtgggt gataacaaga taaaagaatt
      541 accatatctt cctgaaactc taactcattt acggatccat aataaccgat tgcatattct
      601 gccactattg ccaccggagc taaaattact gatagtttct ggaaacagat tagacagcat
      661 cccccccttt ccagataagc ttgaagggct ggctctggct aataatttta tagaacaact
      721 accggaatta ccttttagta tgaaca
//
LOCUS       DAJTYG010000289          722 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTYG010000289 DAJTYG010000000
VERSION     DAJTYG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.292"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..>722
                     /locus_tag="OO568_004190"
     CDS             1..>722
                     /locus_tag="OO568_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643249.1"
                     /translation="MIFIIGFSALPVWAMDCYAEHEEGNTVVIGYVPRISIPSNGKKG
                     DKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSYIFGVTYN
                     GVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPARVQLYIKL
                     AKNANRVNKNLVLPDEYIAPEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLDCFVNVAL
                     EPADGVVDFGKINSRTIKNTTV"
BASE COUNT          230 a          133 c          145 g          214 t
ORIGIN      
        1 attatattta ttataggttt tagtgcatta cctgtatggg caatggactg ctatgctgaa
       61 catgaagagg gaaataccgt agtcattggt tacgtaccaa gaatttctat ccccagtaat
      121 ggtaaaaaag gtgataagat ctggcaaagc agtgagtatt ttatgaatgt cttctgtaat
      181 aatgcattac ctgccccatc acctggagaa gaatacccat cagcctggac taatataatg
      241 atgtttttac caggcggtca ggacttttat aatcagaact cctatatttt tggcgtaaca
      301 tacaatggag ttgattatga ttcgactgcc cctaatgcta tcgcagcacc agaatgcatt
      361 gatataaaag gagcgggtac ctttaataat cactataaaa accccgctgt ctgtagtgga
      421 ggtcctgagc ctcaattatc agtaaccttt ccagctcgcg tacaactcta tattaaatta
      481 gcaaaaaatg ccaatagagt taataaaaac ctggtgttac ctgatgaata tatcgctcca
      541 gagtttaaag gtatgagtgg cgcaggagct atagaagtag acaaaaattt gaccttcagg
      601 attcgtggat taaataatat tcatgtcctt gactgctttg ttaatgtcgc actggaacca
      661 gctgatggcg ttgttgactt tggtaaaata aattcccgaa caattaaaaa taccaccgtt
      721 ag
//
LOCUS       DAJTYG010000290          709 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTYG010000290 DAJTYG010000000
VERSION     DAJTYG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.293"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..444
                     /locus_tag="OO568_004191"
     CDS             <1..444
                     /locus_tag="OO568_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6643250.1"
                     /translation="KKELTEPQIREGSWRKERRTSYSREFKVRLAKQALQPGAVVARI
                     AREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHD
                     ERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            441..>709
                     /gene="tnpB"
                     /locus_tag="OO568_004192"
     CDS             441..>709
                     /gene="tnpB"
                     /locus_tag="OO568_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6643251.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTP"
BASE COUNT          181 a          180 c          206 g          142 t
ORIGIN      
        1 aagaaggaat taacagagcc tcaaatcaga gaaggctcat ggcgaaaaga acggcgaacc
       61 agctatagcc gtgaatttaa ggtccgtctg gctaagcagg cgttacagcc tggggctgtt
      121 gttgcccgga tcgccagaga acacgatatc aataataacc tgctgtttaa atggaaaagc
      181 cagtacgagg acggcttact gagcgatgat gacatacagg aatgcatgcc tgtcccggtg
      241 gcactgactg atacgccgga gccgaccaga ccagttacaa atcccttctg gcgtaacaag
      301 catgatgagc gccctgaggg ggctcccgga aacgtcccac ggtgcgagct gcatcttaaa
      361 tcaggtgtgg taaaactgtt tgaccctctc actccggaac tgttacgggc gctaatccgc
      421 gaaatgaaag ggggtatccg atgataacgc tgccgaccgg taccaaaatc tggatcatcg
      481 ctggcatcac agatatgcgt tgtggcttca atggcctggc ttcgaaggtg cagaacacgc
      541 tgaaagatga cccgttctcc gggcatatct tcgtcttccg gggccgcagt ggcaaaatgg
      601 tgaaaatact gtgggccgat cgtgacgggt tatgcctgtt cgccaaacgc ctggaacggg
      661 gccgcttcgt ctggccggtg acccgggaag ggaaagtgca cctgacgcc
//
LOCUS       DAJTYG010000291          707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTYG010000291 DAJTYG010000000
VERSION     DAJTYG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.294"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            163..>707
                     /locus_tag="OO568_004193"
     CDS             163..>707
                     /locus_tag="OO568_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HCR6643252.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSV"
BASE COUNT          151 a          173 c          196 g          187 t
ORIGIN      
        1 tacagggcgg cgattaccag gctatgatca aatcagcaaa tcagggcgtc tggacatcag
       61 ttgacgtgct gttacaatcg cccacaccta aacaggcgga tacggtatcg ttccgtcatg
      121 gatggcaaac tgcataagcc ataaaaaaac agggagacat ttatgaagct tcgcctgtcg
      181 gcgcttgctc tgggaactac gcttctggtg gggtgtgcga gttccggtac agatcagcaa
      241 gggcgttctg acccgttaga agggttcaac cgcaccatgt acaacttcaa cttcaacgta
      301 ttagacccgt atattgttcg accggtcgct gtcgcctggc gtgattatgt tccgcaaccg
      361 gcgcgtaatg gtttgagcaa ctttactggc aaccttgaag aacctgcggt gatggttaac
      421 tacttcttgc agggcgaccc ttatcagggg atggtccact ttacccgctt tttcctgaac
      481 accattttgg ggatgggcgg ctttattgat gttgcaggga tggcgaaccc gaaactgcaa
      541 cggactgaac ctcaccgctt cggtagtacg cttggtcatt atggcgtggg ttatgggcct
      601 tacgttcagt taccgttcta cggtagcttc acgctgcgtg atgacggtgg tgatatggcg
      661 gatggtcttt acccggttct ttcctggctg acctggccga tgtctgt
//
LOCUS       DAJTYG010000292          664 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTYG010000292 DAJTYG010000000
VERSION     DAJTYG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.295"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..608)
                     /locus_tag="OO568_004194"
     CDS             complement(<1..608)
                     /locus_tag="OO568_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6643253.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RTEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRA"
BASE COUNT          135 a          201 c          158 g          170 t
ORIGIN      
        1 gcccgaattg aagccaaatc atccagaccg ctcagctcct ctttcatttc acgctgacgg
       61 cgataaatct catcgttgcg atcgacctgc gcctgcacca ttgctgccgc cagttcttcc
      121 agttccggca tcgacagttt cacctgctga ttatccgcat cgctccacgc catatgtgtt
      181 tgtgctgtga cagattttgc cagcatgact accggggaca ggcggcccag tgagtcggga
      241 ccagcattcc agatacgacc gttccattca aacgtgaacg gcttcgcctc ctgttctgtg
      301 cgccatgctt caatttcctg acgtctggcc tctctggccg cttccagcat ttctggtgtc
      361 acagtgaatg gggctatctc accccatttg ccactttgca gttcctgcca gatttgctga
      421 cccgtcggtg cgacatcatc agcggtggct gtgtagggga ctgcctggtc cctgtcgtca
      481 aaaaaaacgt cacagtctac tgcgccactt tcggtataac ggggattaat gattttttta
      541 atttccacgg tgcattcctc acgatgtgcg aataaaaagc ccgggcattg cgccagagac
      601 atgtgcatcc ggcaccccgg acagggcgca atatgacccc ggtaatgaat gctctgaaca
      661 tccc
//
LOCUS       DAJTYG010000293          662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTYG010000293 DAJTYG010000000
VERSION     DAJTYG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.296"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..662
                     /locus_tag="OO568_004195"
     rRNA            <1..662
                     /locus_tag="OO568_004195"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          165 a          158 c          199 g          140 t
ORIGIN      
        1 cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg cccgcacaag
       61 cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt cttgacatcc
      121 acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt gctgcatggc
      181 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
      241 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      301 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      361 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      421 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      481 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      541 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      601 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcacctcc
      661 tt
//
LOCUS       DAJTYG010000294          639 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTYG010000294 DAJTYG010000000
VERSION     DAJTYG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.297"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..>639
                     /locus_tag="OO568_004196"
     CDS             <1..>639
                     /locus_tag="OO568_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6643254.1"
                     /translation="DARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAA
                     QTTQASNRIRGLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQL
                     CKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVE
                     QRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTR"
BASE COUNT          124 a          221 c          168 g          126 t
ORIGIN      
        1 ctgatgctcg tgacgctgcc atcattgccg aagctgcccg taccctgcct cacgcgctac
       61 gcacgctgaa actggctgac gagcaaatcg ccgaactctc catgctctgc ggcttcgatg
      121 atgatcttgc cgcacagaca acgcaggcca gcaaccgtat ccgcggcctt ctgacccaga
      181 tacatccggc actggagcgc gttctcggtc cgagacttga gcacccggcg gtactcgatc
      241 ttctccagcg atatccctca ccagaaaaac tcgcttcgct gggtgagaag aagctggcag
      301 cccagctctg caaacttgcg cctcgtctgg gtaaacgcct tgcagcagac atagctcagg
      361 cactggccga acaaaccgtc gtcgttcccg gcacgaatgc cgctgccgta gtactgccac
      421 gtctggcact ccagctcatc acgctgcgta agcaaagaga cgaggtggcg cttgaggtag
      481 aacagcgagt tcttgctcac cctctttacc cggtcctgac cagtatgccc ggagtcggtg
      541 tcaggaccgc agccagactc ctcaccgagg tcgcctgccg cgccttcgcc tctgccgcac
      601 atctcgctgc ttatgctggc cttgcgccgg taactcggc
//
LOCUS       DAJTYG010000295          635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTYG010000295 DAJTYG010000000
VERSION     DAJTYG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.298"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            118..>635
                     /locus_tag="OO568_004197"
     CDS             118..>635
                     /locus_tag="OO568_004197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643255.1"
                     /translation="MKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRRARQVLSQLR
                     SAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIERSRASTSNK
                     LLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIFEQIKPFLEK
                     AMTMYNDVLFPG"
BASE COUNT          174 a          146 c          163 g          152 t
ORIGIN      
        1 tttgaagaga aggtggagaa ctccatgacg gtcgcccagt gtctcgacta ctggttcgac
       61 aactacgtct ctacaactct cagagaaaag gcctaggcac tttatcgatc aacggttatg
      121 aagcgcatgc atgacgcctt ccctaatcgt ccggcatctt ctatcacggt taagcaatgg
      181 gttgacctgc ttaccgaaga agaaaaagat aatccacgcc gagcaaggca ggtgctaagt
      241 caactaagat cagcaattag ttggtgcatg cggcgtcagt tgatagatag ttgcgcaatt
      301 atgagcatcc agccaaggga cttcggctcc cgcgctgagg taggggatcg ggtactgtcg
      361 tatcacgaac tggctaagat ttggcttgct attgaaagaa gccgtgcgtc tacgtcaaat
      421 aagctccttc atcagatgct tatgctgtgg ggggcgaggc tctcagagct taggctggca
      481 acaaagacag aatttgacct gctggacaac gtatggaccg taccgaaaga gcatagcaag
      541 atgggtaatg ttatccgccg tccaatcttc gaacaaatta agccttttct cgaaaaggcc
      601 atgacaatgt acaatgatgt tcttttccct ggaga
//
LOCUS       DAJTYG010000296          632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTYG010000296 DAJTYG010000000
VERSION     DAJTYG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.299"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..224
                     /locus_tag="OO568_004198"
     CDS             <1..224
                     /locus_tag="OO568_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCR6643256.1"
                     /translation="PQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTVL
                     DGKVIDERTTSHINRMQEDTLISSAYPEE"
BASE COUNT          218 a          121 c          115 g          178 t
ORIGIN      
        1 ttccacagtt ttcacctgaa gcattaaata atctgaaagg tcgagtggaa acattcacac
       61 aagatttaaa ggttatcaaa ccgtcagtaa atattcagcc tcagacaatc aatatcacaa
      121 ctcagacggt acttgatgga aaggttattg atgaaagaac cacatctcat attaatcgta
      181 tgcaggaaga taccttgatt agctcagctt acccagagga ataaacaatg ttagaactac
      241 taatatttat tgccacacat ggcaatctat taatctttat ctagtatgac gattcaacta
      301 tgaatagtcg aatgctacgt gatttcttct gagcagatac caacatcaaa aattagattt
      361 cacgtagctc acgcttaaca gaataggaat aacaacaatg tttgacacaa agcaaattag
      421 atactcaggt aaagatggta tctattttca ccttagagat aatgtagaat cttttcttgc
      481 cttaacagct actgaacaga tggactatga ctcaccgatg atggtgacaa ctcaggctat
      541 gcaatcgggg caaactatca ctgataatgt tcagagagca cctaagacca tctctattag
      601 cggtgttgta gtggttgatt cgacgggttc ag
//
LOCUS       DAJTYG010000297          602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTYG010000297 DAJTYG010000000
VERSION     DAJTYG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.300"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
BASE COUNT          213 a          107 c          128 g          154 t
ORIGIN      
        1 gctgatgggg cgcttacatt aatcccgtac tctctggcca gttgggccac gcaggcccct
       61 agctggtaag atttttcaat aagatcaagc ttaaactcca tagagtcata gagtaagagg
      121 cacgaatcct gcgggggcga agagaatctg acattgatag agtccattaa cgttataaga
      181 ggactctatc atcgccaatc atcagacatt gaaatagacg acactgagat gacgcttaca
      241 attcttttgg tttctgtaat aagtaatctg acattccccg tgcccagaag aggagaggga
      301 tatcaagccc aagggttaat aattgatact acggttaaaa ttgccttcag taaaggaaac
      361 gtgattaaat tcaggggatt tcagagtgaa aaccatacaa catattaaaa tctgttcatt
      421 tagctataat acatacaaga aagtgtccag ttagataatg ttatctaaat aaccacagat
      481 aaaaacgcac ataattgggg gggggggatg aaaatacctg aagcagtaaa tcatattaat
      541 gttcagaata atattgatct tgttgatgga aaaacgaatc cgaataaagc aacaaaagca
      601 tt
//
LOCUS       DAJTYG010000298          600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTYG010000298 DAJTYG010000000
VERSION     DAJTYG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.301"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..600
                     /locus_tag="OO568_004199"
                     /pseudo
     CDS             1..600
                     /locus_tag="OO568_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          137 a          160 c          175 g          128 t
ORIGIN      
        1 gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcact gccggacatc
       61 agcgctatct ctgctctcac tgccgtaaaa catggcaact gcagttcact tacaccgctt
      121 ctcaacccgg tacgcaccag aaaatcattg atatggccat gaatggcgtt ggatgccggg
      181 caactgcacg cattatgggc gttggcctca acacgatttt acgtcactta aaaaactcag
      241 gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat
      301 ggacgaacag tggggctatg tcggggctaa atcgcgccag cgctggctgt tttacgcgta
      361 tgacagtctc cggaagacgg ttgttgcgca cgtattcggt gaacgcacta tggcgacgct
      421 ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga cggatggctg
      481 gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc gatatacgca
      541 gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg gacggaagtc
//
LOCUS       DAJTYG010000299          594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTYG010000299 DAJTYG010000000
VERSION     DAJTYG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.302"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..>594)
                     /gene="chbR"
                     /locus_tag="OO568_004200"
     CDS             complement(<1..>594)
                     /gene="chbR"
                     /locus_tag="OO568_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR6643257.1"
                     /translation="TAREQQLFNGKNFHVFIYNKTDSISVLHQHDYYEFTLVLTGRYF
                     QEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFEQHYLPLLPYCFV
                     ASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHYREEQVIDDVPQ
                     WLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRAT"
BASE COUNT          182 a          143 c          110 g          159 t
ORIGIN      
        1 agtcgctcgc gtcaaatatt cctgtgattt ggctgacaac gtcaccatat tctccagcgc
       61 cgattcacta aactgctctt tatcatgcat cttttctacc gtacttttca gccactgcgg
      121 tacatcatca atcacctgtt cttcgcggta atgacgtaaa cggttaatga cataaaaagt
      181 aaccatctca acaaactctt ccagccctgt ttcgcggaaa tttaatgaag aaatcactgt
      241 ttccacatag gtgagaaaag cgttattggt ccggtatacc tgcgaagcaa caaagcaata
      301 aggcaacaac ggcaggtaat gctgctcaaa aaagcgttta ctgatcccaa cgttcaatat
      361 gcgcgtggca ccaaactcat aaaaactttg atggtgcgaa cctaacggaa taaaaacaaa
      421 atcgccccgt tccagtaaca cgcgcttacc gttaatctcc tggaaataac gcccggttaa
      481 taccagagta aattcataat agtcgtgctg atgcagtacg ctgatactat cagttttgtt
      541 atagataaac acatggaagt ttttgccatt aaacaactgc tgttctcggg cagt
//
LOCUS       DAJTYG010000300          585 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTYG010000300 DAJTYG010000000
VERSION     DAJTYG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.303"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..433
                     /gene="ospC3"
                     /locus_tag="OO568_004201"
     CDS             <1..433
                     /gene="ospC3"
                     /locus_tag="OO568_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC3"
                     /protein_id="HCR6643258.1"
                     /translation="KLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADI
                     ALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVN
                     KKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          219 a           82 c          113 g          171 t
ORIGIN      
        1 aaaactcaga gacatatctt tggaggatgc gattaaagca tctaattatg aagagattaa
       61 caataaagtg actgataaaa aaatggctca tcaggccctt gcttattctt taggcaataa
      121 aaaagctgat atagcacttt atttactatc taaatttaat tttacaaaac aagatgtcgc
      181 agagatggag aaaatgaaca acaatagata ttgtaatctg tatgatgttg aatacttact
      241 tagtaaagat ggtgccaact ataaagtgct agaatatttt atcaataatg gattggttga
      301 tgtaaacaaa aaatttcaaa aagcaaatag tggggacact atgcttgata atgcaatgaa
      361 aagcaaagat tcaaaaatga ttgatttttt attaaaaaat ggagcaatat taggcaaacg
      421 atttgagata tgacctaaaa gctcggccaa ataataatgt cgtcgattat cgaagtcatc
      481 atgagtgatt ctgtccgcac agtgcagcat cagcatgaca gtgaattgtc tggcgtggtc
      541 catttaatga gatggagatg gtccctcttt cctaaatagc tgtgc
//
LOCUS       DAJTYG010000301          554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTYG010000301 DAJTYG010000000
VERSION     DAJTYG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.304"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(28..>554)
                     /locus_tag="OO568_004202"
     CDS             complement(28..>554)
                     /locus_tag="OO568_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6643259.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGAAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT          144 a          140 c          134 g          136 t
ORIGIN      
        1 accagtattt cttcggagtg aagaatatta ccaagtatat ttaacaccca cgttcgcgga
       61 ccagtcttga tctacgtcac caccaccgag gtagttagca tcggtatagg cgctgaagtt
      121 cttggtgaag ctgaactgag tacccagacc aacacgtacc gcagcccctt cagtaccgtt
      181 atcgatggaa tcaccgttca catcgttatc gttgttagag tcgtcgtaga cgtaagccag
      241 tttgaagtac ggagtcagag cctgatcttc gctgtaggtg aaggtataac ctgcatctac
      301 acccagttca taacgcatgc tgtcgtaaga ctgaccgtca actttcatgt cgttgctcag
      361 ctggtagtca tcaccagact ggaacagacc agaaatgctg ccgtaaggag tcacgtaacc
      421 agcatcaccc agtttgaagt cgtaaccggc tttcaaaccg aagccccaag cgtcggagtt
      481 ggtgctaccg tcaacgtaag taccgttgct catggttgca gacaggtcgt tgttgaagtg
      541 agagtagctc aagc
//
LOCUS       DAJTYG010000302          552 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTYG010000302 DAJTYG010000000
VERSION     DAJTYG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.305"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..>552
                     /locus_tag="OO568_004203"
     CDS             <1..>552
                     /locus_tag="OO568_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643260.1"
                     /translation="CEAHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSG
                     SPDIEMIKNRMAELFNQSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILAT
                     AINETLEGDTNVAVVMGCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGY
                     FSCDSLTPEELMMLKNLFDIRVL"
BASE COUNT          161 a          110 c          128 g          153 t
ORIGIN      
        1 ctgtgaagcg catagccatc gcagcgtcgc ccttgccaaa accgtggctg cgcccatcat
       61 ggcgacaggt gtgggcttgg cattaatgta tgcaacatca tcaggaccgt attatgcgca
      121 aagtctttct ggttcgccgg atatcgagat gataaaaaat cgcatggctg aattgtttaa
      181 ccagtcggat atttctgtgc gcaacaaata tagcccggct tttgataaat ggaatgaact
      241 ggtagataaa ttaaatcacg aaagaaatac agttccgttt gcgtgtttga ccaccatttt
      301 agcgacggcg ataaatgaaa ctcttgaggg ggatacgaat gttgccgttg tgatggggtg
      361 taaatctgcg aaagatcgca cgatatccat tgtccttggc aactcgatgt tgcaaacact
      421 ccttgaaaaa cgcctcgccg atagtggaga aatagaaaat ctttttgatc aacagggata
      481 ttttagttgc gattcattaa cacccgaaga attaatgatg ttaaaaaacc tttttgatat
      541 tcgagttctt ca
//
LOCUS       DAJTYG010000303          546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTYG010000303 DAJTYG010000000
VERSION     DAJTYG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.306"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..535
                     /locus_tag="OO568_004204"
     CDS             <1..535
                     /locus_tag="OO568_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6643261.1"
                     /translation="RRKIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAV
                     RKARSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNI
                     GENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDW
                     PSNQVHELLPWNFSSVK"
BASE COUNT          158 a          119 c          163 g          106 t
ORIGIN      
        1 ccgccgaaaa atccatgacg aggatgtgcg ccgtccgaca gaaatgactc aggaagcgct
       61 cagacggata gcagagttat acgacataga agcggagata cgtggcagtc cggcagagga
      121 acggcttgca gtcagaaaag ccagaagcgt ccagttgatg cagtcgttgt acgactggat
      181 acagttgcag aggaaaacgc tgtcgaaaca tgcggagatg gcgaaggcgt tcgactatat
      241 cctgaatcac tggaatgcgc tgaacgagtt ctgtcgtgac ggctgggtgg aaatagacaa
      301 caacatcggt gaaaacgcgt tacgatcggt ggcggttgga agaaaaaatt atctcttttt
      361 cggctcagac aagggaggag aaagtgcggc gatcatctac agtctgctgg tcacctgcaa
      421 acagaacgaa gtggagccgg aggactggtt gcgcgaagtg atcgagaagc tcaatgactg
      481 gccgtcgaac caagtgcatg aactgctgcc ctggaacttc tcgtctgtaa aataatcctt
      541 acgcta
//
LOCUS       DAJTYG010000304          544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTYG010000304 DAJTYG010000000
VERSION     DAJTYG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.307"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            90..155
                     /locus_tag="OO568_004205"
                     /pseudo
     CDS             90..155
                     /locus_tag="OO568_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            383..>544
                     /locus_tag="OO568_004206"
     CDS             383..>544
                     /locus_tag="OO568_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6643262.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTR"
BASE COUNT          172 a          114 c          125 g          133 t
ORIGIN      
        1 ctatagatgc acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa
       61 actgggacct cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt
      121 tgctttgtga tattaatcct gtcacgttac gcgcgtgacg caacattttt aaaaatcgac
      181 gaagatcgtc caggagcgcc gtttttcaag ggttggatag atgattcgtt taccatgtcg
      241 acgaacttcg acgaatttcg ccgataaaaa gtgtcccata catgccccat tgatgcccca
      301 taaacaatat caggaattga cactggttat ccatacagat aaaaataaca cctgtacata
      361 actacagtta caggagggtg ttatgcgtgt tgagatctgt atcgctaaag aaaaaatcac
      421 taaaatgcca aacggtgctg tggatgcgtt aaaggaagaa ttaacccgac gcatcagtaa
      481 acgttatgac gatgtagagg tgatcgtaaa agccaccagc aacgatggcc tttctgttac
      541 acgc
//
LOCUS       DAJTYG010000305          527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTYG010000305 DAJTYG010000000
VERSION     DAJTYG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.308"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..142
                     /locus_tag="OO568_004207"
     CDS             <1..142
                     /locus_tag="OO568_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6643263.1"
                     /translation="LEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHR
                     PQ"
     gene            complement(143..526)
                     /locus_tag="OO568_004208"
     CDS             complement(143..526)
                     /locus_tag="OO568_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6643264.1"
                     /translation="MLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFI
                     AVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLAL
                     NVATPFVLVGLGLIFPLLARKANSK"
BASE COUNT          158 a          142 c          112 g          115 t
ORIGIN      
        1 actggaagac tggatcacag agcgtccagg cttccaagtc tattacccaa accgccagta
       61 tatgtcctgc ggcttgcgcg catttctcga ttatgtaaaa acgggtcaga tctgccagag
      121 tcagcgtcac cgaccacaat aattatttgc tattcgcttt acgcgccagc agcgggaata
      181 tcagtcctaa tcctaccagc acaaacggcg ttgcaacatt cagcgccaac tggaaggtcc
      241 actctgcagt gaaggcttcc attttcggga agatccccgt caaacaggca caggcggtaa
      301 aggcaaaaca ccaaatcccg acggtcattg ccagaggctt attacgaata aagacatatt
      361 ctggtttata tttctgcgcc aggcgaacga ctgcaataaa tgcaacaaat acccataaat
      421 aacgcagcgg catcactacc gagttaagat tcaacagcca tttatagaga ttgttcatat
      481 caccgatgcc gagagtcggc agcataatca gaattgccac cagcacc
//
LOCUS       DAJTYG010000306          527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTYG010000306 DAJTYG010000000
VERSION     DAJTYG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.309"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(66..266)
                     /gene="glgS"
                     /locus_tag="OO568_004209"
     CDS             complement(66..266)
                     /gene="glgS"
                     /locus_tag="OO568_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR6643265.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRLVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          173 a          123 c           82 g          149 t
ORIGIN      
        1 tcctcaaaat cacctataac tagcatctaa tacaaacact aaaacgggcc atcaggcccg
       61 ttatatcagt gctctaactc cagctctctt gcctgcatca tctgttggca ataccaggca
      121 taacgtgcgc aaaaccaggt tctatgttct tcatccatgt taccagtaac ggccagaata
      181 tcgaccaggc gaccttctgc atgcattctg gcaaaactac gcgccaggaa atcgaaatta
      241 ttcaaagaat taagactatg atccataagc acatcctccg gttatcgcat accttttaag
      301 gccatatgct ttttctgatt tcagtattcc ccaatcaggg tagtgccttt aaacataacg
      361 tgcgtaaata tattgtcccc gatcacactt tttagtgaaa aataaaaaac catattaaaa
      421 tactttctct tccagaactt ctctaacatg tgttattatt aacaaagaaa aatgtaagaa
      481 gggaaattat tccaaggtaa atattgcgtc actaaatgga cattgga
//
LOCUS       DAJTYG010000307          525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTYG010000307 DAJTYG010000000
VERSION     DAJTYG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.310"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..189)
                     /locus_tag="OO568_004210"
     CDS             complement(<1..189)
                     /locus_tag="OO568_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HCR6643266.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQT"
     gene            254..445
                     /locus_tag="OO568_004211"
     CDS             254..445
                     /locus_tag="OO568_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6643267.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
BASE COUNT          128 a          159 c          136 g          102 t
ORIGIN      
        1 tgtctgattc aggttattcc cgatggcagc cagctgacgc agtaacggcg gtgccagtgt
       61 cggcagtttc ccggaacggg caaccggctc acccaggcag acccgccgca tccataccgc
      121 cagctgttta ccctcacagc gttccagtaa ccgggcatgt tcatcatcag taacccgtat
      181 ggtgagcatc ctctcgcgat tcatcggtat cattacccca taaaacagaa atccccctta
      241 cacggaggca tcagtgacta aacaggaaaa aaccgccctt aacatggccc gctttatcag
      301 aagccagaca ttaacgctgc tggagaagct caacgaactg gacgcggatg aacaggctga
      361 tatctgtgaa tcgcttcacg accacgccga tgagctttac cgcagctgcc tcgcacgttt
      421 cggggatgac ggtgaaaacc tctgacatat gcagctcccg gagacggtca cagcttgtct
      481 gtgagcggat gccgggagca gacaagcccg tcagggcgcg tcagc
//
LOCUS       DAJTYG010000308          516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTYG010000308 DAJTYG010000000
VERSION     DAJTYG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.311"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..>516)
                     /locus_tag="OO568_004212"
     CDS             complement(<1..>516)
                     /locus_tag="OO568_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6643268.1"
                     /translation="DCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNNAADTHV
                     VDVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSSEVFNNT
                     ITVKDSTVTSGSWTDEGTTGWFGNTGNASDYSGKSNFVTVDTDGDGVADSTIASWDDV
                     ALAVVAHPNADN"
BASE COUNT          147 a          122 c          103 g          144 t
ORIGIN      
        1 attatcagca ttcggatgtg caactacagc taatgcaaca tcatcccagc ttgcaatagt
       61 gctatcagca acaccatctc cgtcagtatc tacggtgaca aagttagatt taccactata
      121 atcgctggca ttacctgtat taccgaacca accagtagta ccttcatcag tccatgaacc
      181 agaggttacg gtagaatctt taacagtgat ggtgttatta aacacttcgc tgctgtttac
      241 gccagtgctg acagtgttgt cgtcaatatc ttcccactca taaccctgag tcaacgtaat
      301 acccgcaacg tgagagttat tagtgatgga cagatcaact tcctgatcca gagtaatagc
      361 cgtaccaatg ttgtaaacgt ctacaacatg agtatcagcg gcattattgt aagtgccgtt
      421 atatgtgtag tgttcgtagt tgtcgtcgat cgttgaatta tcaacagtca gcgccagacg
      481 gtcatggtag taatcatccg cacgatcagc acaatc
//
LOCUS       DAJTYG010000309          509 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTYG010000309 DAJTYG010000000
VERSION     DAJTYG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.313"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..276
                     /locus_tag="OO568_004213"
     CDS             <1..276
                     /locus_tag="OO568_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6643269.1"
                     /translation="LNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGFDNGGESAA
                     IIYSLLFTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          124 a          113 c          135 g          137 t
ORIGIN      
        1 ctgaacgtgt actgtcgtaa tggccaggtg gaaatcgaca acaacatcgg tgaaaacgcg
       61 ctgcgatcgg tggcggttgg aagaaaaaat tatctcttct tcggttttga caacggtgga
      121 gaaagcgcgg cgattatcta cagtctgctg ttcacctgca aactgaacgg agtggagccg
      181 gaggactggt tgcgcgaagt gatcgtgaag ctcaatgact ggccgtcgaa ccgagtgcat
      241 gaactgctgc cctggaactt ctcagctgta aaataatcct tccgctacgt acttctctgg
      301 gcgcttacca ctcaacggca tcgtcaaaat tatcaaagcg cagaacatag atttatcatg
      361 tgttttatgg atggctttct ccatcaggca tcgttcgtca cgggaaattg gtgctatgat
      421 cggcattgat cagtccggtt ggtgatttgt tttgatttgg cgattgatca gatcgcacaa
      481 tccgggctga gttccctcta agtgatcta
//
LOCUS       DAJTYG010000310          497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTYG010000310 DAJTYG010000000
VERSION     DAJTYG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.314"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..>497)
                     /locus_tag="OO568_004214"
     CDS             complement(<1..>497)
                     /locus_tag="OO568_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6643270.1"
                     /translation="YTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAG
                     TGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQ
                     FSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTN
                     AQMVS"
BASE COUNT          113 a          116 c          111 g          157 t
ORIGIN      
        1 ggataccatt tgtgcgtttg tgtggcccat ctggcttgcg ataaaactag gattagctcc
       61 tgcggcaagt gaccagcatg cataggtatg cctggattga tacgattttc tgtgtctcag
      121 acctgcgcgt tttaagatac ttgtccatga ctccctgatg gagtcaacct tatagtgcgg
      181 tccggaaaac tgccgctgtt ttattacctg aggactaaaa acaaaagtgc atttatgcac
      241 agtacttctc ccatattccc tctgctttac ctctacagaa tgctgttttc caagcatggt
      301 catttccgct tggcttttaa gagcatcaat agctggttga actagatgaa tcgttcttcc
      361 ggtaccagca tcagtttttg gtggagtgaa ttcgcctagt tttgtataat tccggcggat
      421 ggttatagtc ctcgctttaa ggtctatatc ttcccaggcc agtgatacca gctccccgtg
      481 acgaataccc gtgtata
//
LOCUS       DAJTYG010000311          495 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTYG010000311 DAJTYG010000000
VERSION     DAJTYG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.316"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..>495)
                     /locus_tag="OO568_004215"
     rRNA            complement(<1..>495)
                     /locus_tag="OO568_004215"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           92 a          165 c          109 g          129 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg
      301 ggtaacgtca atgagcaaag gtattaactt tactcccttc ctccccgctg aaagtacttt
      361 acaacccgaa ggccttcttc atacacgcgg catggctgca tcaggcttgc gcccattgtg
      421 caatattccc cactgctgcc tcccgtagga gtctggaccg tgtctcagtt ccagtgtggc
      481 tggtcatcct ctcag
//
LOCUS       DAJTYG010000312          469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTYG010000312 DAJTYG010000000
VERSION     DAJTYG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.317"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..>469
                     /locus_tag="OO568_004216"
     CDS             <1..>469
                     /locus_tag="OO568_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR6643271.1"
                     /translation="RDAGTSASEAATNATDAAGSARAASTSAGSAASSASSASASKDE
                     ATKQASAAKGSATTASSKATESAGSATAAAQSKSAAESAAMRAETAAKRAEDIADVVS
                     LEDASTTKKGIVQLSSATNSASESLAATAKAVKVVMDETNKKAPLNSPALTGTP"
BASE COUNT          127 a          128 c          151 g           63 t
ORIGIN      
        1 cagggatgcc ggcacatctg ccagtgaggc ggcaaccaat gcgactgatg ctgcaggctc
       61 agcacgcgca gccagcacgt cagccggttc ggcagcgtca tcggcatcat ctgcgtctgc
      121 ttcaaaagat gaggcgacta aacaggcgtc agcagcgaag ggcagtgcca cgacggcatc
      181 ctctaaggcg acagagtctg caggcagtgc gacggcggca gcgcagagca aaagcgcggc
      241 agaatccgcg gcaatgcgcg ctgagacagc agcaaaacgg gcagaggata ttgctgatgt
      301 cgtatctctg gaagatgcga gcacgacgaa aaaggggata gtgcagctca gcagtgcgac
      361 caacagtgca tccgagtcac tggccgcgac agcgaaagcg gttaaggtgg taatggatga
      421 aacaaacaag aaagcgcccc tgaacagtcc ggctctgacc ggaacgcca
//
LOCUS       DAJTYG010000313          469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTYG010000313 DAJTYG010000000
VERSION     DAJTYG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.318"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
BASE COUNT          132 a           95 c           86 g          156 t
ORIGIN      
        1 tctgactcgc aattcatcca gcagtttatc atttcccata atgtcactta ccctcatcgg
       61 taaaaaaatt gcctttcgac cattacgata cattattgat tttggggttt cagggaagta
      121 atccatttcg actataactg acaggtcatc acgacgtatg actgcgtatt tgctactaaa
      181 tagtttcttt attttttcca cgatgcctcc aggtttataa gtacaaacgg ttatatccac
      241 atagagacaa aaatattaat ctgaaaaata tttatttcac gccgtatatt tgattgttta
      301 atgtgcaagt acaatgactt ttattttttg ttgtgtatat aatcaaatat atggttattt
      361 ttcaccctgc gtattcagca cgcaacaaaa aacccgccga agcgggttta gtgcgggtgc
      421 gttgaggatg cctgacacat gagaggtggc gagggatttc tcccccgcc
//
LOCUS       DAJTYG010000314          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTYG010000314 DAJTYG010000000
VERSION     DAJTYG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.319"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..>464
                     /locus_tag="OO568_004217"
     CDS             <1..>464
                     /locus_tag="OO568_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6643272.1"
                     /translation="QHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQ
                     RWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKL
                     HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY"
BASE COUNT          113 a          111 c          133 g          107 t
ORIGIN      
        1 ctcaacacgg ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
       61 aaccgggcag tgatgtgatt gtctgcgctg aaatggacga acattggggc tacgtcggtg
      121 ctaaatcacg tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg
      181 cgcacgtctt cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg
      241 cctttgaggt cgtggtatgg atgacggatg gctggccgct gtatgaatca cgcctgaagg
      301 gaaagctgca cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc
      361 tgagacaaca tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc
      421 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctat
//
LOCUS       DAJTYG010000315          459 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTYG010000315 DAJTYG010000000
VERSION     DAJTYG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.320"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..>459)
                     /locus_tag="OO568_004218"
     rRNA            complement(<1..>459)
                     /locus_tag="OO568_004218"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          100 a          149 c          106 g          104 t
ORIGIN      
        1 cctcacggtt cattagtacc ggttagctca acgcatcgct gcgcttacac acccggccta
       61 tcaacgtcgt cgtcttcaac gttccttcag gacccttaaa gggtcaggga gaactcatct
      121 cggggcaagt ttcgtgctta gatgctttca gcacttatct cttccgcatt tagctaccgg
      181 gcagtgccat tggcatgaca acccgaacac cagtgatgcg tccactccgg tcctctcgta
      241 ctaggagcag cccccctcag ttctccagcg cccacggcag atagggaccg aactgtctca
      301 cgacgttcta aacccagctc gcgtaccact ttaaatggcg aacagccata cccttgggac
      361 ctacttcagc cccaggatgt gatgagccga catcgaggtg ccaaacaccg ccgtcgatat
      421 gaactcttgg gcggtatcag cctgttatcc ccggagtac
//
LOCUS       DAJTYG010000316          454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTYG010000316 DAJTYG010000000
VERSION     DAJTYG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.321"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            28..454
                     /locus_tag="OO568_004219"
                     /pseudo
     CDS             28..454
                     /locus_tag="OO568_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          117 a          109 c          131 g           97 t
ORIGIN      
        1 gtttttagac tagtcattgg agaacagatg attgatgtct tagggccgga gaaacgcaga
       61 cggcgtacca cacaggaaaa gatcgcaatt gttcagcaga gctttgaacc ggggatgacg
      121 gtctccctcg ttgcccggca acatggtgta gcagccagcc agttatttct ctggcgtaag
      181 caataccagg aaggaagtct tactgctgtg gccgccggag aacaggttgt tcctgcctct
      241 gaacttgctg ccgccatgaa gcagattaaa gaactccagc gcctgctcgg caagaaaacg
      301 atggaaaatg aactcctcaa agaagccgtt gaatatggac gggcaaaaaa gtggatagcg
      361 cacgcgccct tattgcccgg ggatggggag taagcttagt cagccgttgt ctccgggtgt
      421 cgcgtgcgca gttgcacgtc attctcagac gaac
//
LOCUS       DAJTYG010000317          453 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTYG010000317 DAJTYG010000000
VERSION     DAJTYG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.322"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            2..>453
                     /locus_tag="OO568_004220"
     CDS             2..>453
                     /locus_tag="OO568_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6643273.1"
                     /translation="MPVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGND
                     LKELRVALNYSQNPVSPKARSLILWTERGAARHAKMLETDQAWDVFEKLEDCYFSQRD
                     PSTPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARA"
BASE COUNT          123 a          110 c          107 g          113 t
ORIGIN      
        1 gattcctgtt attaccaccg aacttttggc gcagctttac ggcactgagc cggtgcgtat
       61 tcgccagaat catcatgaga acaaagtacg cttcgttgaa gggaaacact ttttcaaagt
      121 tgttggtaat gaccttaaag aattgcgggt cgctttaaac tactcacaaa atccagtttc
      181 acccaaagcc cgctccctca tcctctggac agaacgaggc gcagcacgcc acgccaaaat
      241 gctcgaaacc gatcaggcgt gggatgtgtt cgaaaaactg gaagactgct atttcagtca
      301 aagggatccg tcaacgccag tttcatgcca gaaaagttac gacacgcgag ttctctgtta
      361 tcagagaggc ggtgtcactg tttccacaat tcagttacgg gatgatgata ttgttatttc
      421 ccttgagtca tggctggaac tggcgagagc caa
//
LOCUS       DAJTYG010000318          439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTYG010000318 DAJTYG010000000
VERSION     DAJTYG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.323"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..359
                     /locus_tag="OO568_004221"
     CDS             <1..359
                     /locus_tag="OO568_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR6643274.1"
                     /translation="IAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTY
                     HGMLYRMCALVVISEITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKG
                     FLASISRGVMPSLPFL"
BASE COUNT          109 a           85 c          105 g          140 t
ORIGIN      
        1 taatcgcgca gttaatttat tattttggga ttgctgttag caccagttat ttaacggtgt
       61 cgttgcgaaa tcattttatg agcaacctgt cactgaatga tgggcgtatt cgttttcgct
      121 caactttaac gtaccacggt atgctttatc gcatgtgtgc gttggtggtg atatccgaga
      181 ttacgggtgg tctggcttac ccactgctga aaatatggat gattgactgg caggcaaaaa
      241 atacgtattt gctgggcgat ttggatgacc ttcctttaat caataaagaa gaacaaccag
      301 ataaaggctt cttagccagt atttctcggg gagttatgcc ttctttacca tttctgtaag
      361 agataaaaaa ggccggagca tgctccggcc ttcgttttca tcactgtgtt gattacagtg
      421 cggcgataac agcctgctg
//
LOCUS       DAJTYG010000319          430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTYG010000319 DAJTYG010000000
VERSION     DAJTYG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.324"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            210..>430
                     /locus_tag="OO568_004222"
     CDS             210..>430
                     /locus_tag="OO568_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6643275.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNA"
BASE COUNT          136 a          104 c           59 g          131 t
ORIGIN      
        1 cgaacctcgc ttaccaacgc gttgtcagtc atctcccccc tccttggtgc atcgttatat
       61 ccgtgatgag cgatttttga tattaatatc tttcaccatc cgctattttc accacttcag
      121 aaaacaaact tatttttatt aaaatcaatt ctttttcttt gaacattcaa ctgcgatatt
      181 tgtaaaaaca ttaaacaggc aacaataaaa tgacattacc aacaactatt tattcattcc
      241 ccgcatattt accccgtttt tcgtccaccg acaaaccagc aaaattaaaa tttcatcaat
      301 acgcacgcgc tacattactg tcaaatcgtg gaagagatca taattgcgat ggcagacgta
      361 ctgttgagat ccacaaacta gatctctctg actggcaagc attcaataag ttagcaacga
      421 agtgtaatgc
//
LOCUS       DAJTYG010000320          422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTYG010000320 DAJTYG010000000
VERSION     DAJTYG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.325"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(284..>422)
                     /locus_tag="OO568_004223"
     CDS             complement(284..>422)
                     /locus_tag="OO568_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6643276.1"
                     /translation="TWYYFNKNMNIYTAYQINLLDKSDYVLAHGLNTDDQLAVGIVYQ
                     F"
BASE COUNT          115 a           66 c          113 g          128 t
ORIGIN      
        1 aaagcttttt tcatgcacta tttttgcaaa tatttctata tgggaagcgg ttttgttaca
       61 gattaaattt ataaagaaat gcatttgggg tgttgggttg aagagtagct ggtagatgtt
      121 attgtgccag caggggcagg ggaatgtgcc gtatcgatat gctttggcaa ttgcgtgtgc
      181 cgcagatgga gatgaaatgc caataaaaaa gcctgagttc gatgagggac gaactcaggc
      241 tggtgttatg ttttacagta acacgcctcg gcgcgttggc tgattagaac tggtagacga
      301 taccaactgc cagttggtcg tcggtgttca gtccatgagc gagaacataa tcactcttat
      361 caagcaggtt gatctgataa gcggtatata tgttcatatt cttattgaag taataccagg
      421 ta
//
LOCUS       DAJTYG010000321          414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTYG010000321 DAJTYG010000000
VERSION     DAJTYG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.326"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..>414
                     /locus_tag="OO568_004224"
     CDS             <1..>414
                     /locus_tag="OO568_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6643277.1"
                     /translation="AFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACTLVAATKD
                     SVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADGVQPTAFA
                     NQATTDVATNVALQMYLPDGSTSVTPGTETSNIQLA"
BASE COUNT          111 a           93 c           98 g          112 t
ORIGIN      
        1 ggcgtttata actgtggtat gtgcaacgtc cagcgttatg gctgctgatg ataatgctat
       61 cacggatggc tcagtaacat ttaatggtaa agttattgct ccagcttgta ccctggtagc
      121 tgcgacgaaa gattcagtag tcacattgcc aaatgtcagc gccaccaagt tgcaaactaa
      181 tggagcagtc tctggcgtta aaactgatgt accaattgcc ttagaaggct gtgatgttac
      241 tgtaactaaa aatgcgacgt ttacctttag tggtacggct gacggcgtgc aacctacagc
      301 atttgccaac caggctacca ccgatgtagc gacgaatgtt gctctacaaa tgtatctgcc
      361 agatggttct acatcggtaa ctcccggtac cgaaacgtcg aatattcagt tagc
//
LOCUS       DAJTYG010000322          402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTYG010000322 DAJTYG010000000
VERSION     DAJTYG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.327"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..91
                     /gene="yjdI"
                     /locus_tag="OO568_004225"
     CDS             <1..91
                     /gene="yjdI"
                     /locus_tag="OO568_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR6643278.1"
                     /translation="WVMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            103..375
                     /locus_tag="OO568_004226"
     CDS             103..375
                     /locus_tag="OO568_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6643279.1"
                     /translation="MEICEGHNNFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT          132 a           68 c          109 g           93 t
ORIGIN      
        1 gtgggtcatg ccggatgaag tcgacgtcgc cactgtggtt aaagtgattg atacgtgccc
       61 gagcggcgcg ctgaaatacc gtcataaata agcgagggta aaatggaaat atgcgaaggc
      121 cacaataatt tttacattaa tgacgaacaa ggcaagcaaa tcgctgaaat tgtctttgtg
      181 ccgaccggag agaatttagc gattatcgaa cataccgatg tcgatgaaag cctgaaaggg
      241 caagggattg gtaaacagct ggttgcgaaa gtcgtggaaa aaatgcgtcg ggaaaaacga
      301 aaaattatcc cattatgtcc atttgcgaaa cacgaatttg ataaaacgcg ggagtatgat
      361 gatattcgca gttgatggga gagtacagag tcacgatatt tt
//
LOCUS       DAJTYG010000323          394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTYG010000323 DAJTYG010000000
VERSION     DAJTYG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.328"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..333
                     /locus_tag="OO568_004227"
     CDS             <1..333
                     /locus_tag="OO568_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6643280.1"
                     /translation="GKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETASRHGLRWLHD
                     VANQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVSFDNPPQHHPLSIY
                     DSFCRGVA"
     gene            330..>394
                     /locus_tag="OO568_004228"
     CDS             330..>394
                     /locus_tag="OO568_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6643281.1"
                     /translation="MMMELQHQRLMALAGQLQLESL"
BASE COUNT           98 a          114 c          106 g           76 t
ORIGIN      
        1 ggtaaggtgg aacggatggt gcagtacacc cgtaacagtt tttacatccc actaatgact
       61 cgcctgcgcc cgatggggag cactgtcgat gttgaaacag ccagccgcca cggtctgcgc
      121 tggctgcacg atgtcgctaa ccaacgaaag catgaaacaa tccaggcccg tccctgtgat
      181 cgctggctcg aagagcagca gtccatgctg gcactgcctc cggagaaaaa agagtatgac
      241 gtgcatcctg gtgaaaatct ggtgagcttc gacaaccccc cccagcatca tccactctcc
      301 atctacgact cattctgcag aggagtggcg tgatgatgga actgcaacat caacgactga
      361 tggcgctcgc cgggcagttg caactggaaa gcct
//
LOCUS       DAJTYG010000324          370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTYG010000324 DAJTYG010000000
VERSION     DAJTYG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.329"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..>370
                     /locus_tag="OO568_004229"
     CDS             <1..>370
                     /locus_tag="OO568_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6643282.1"
                     /translation="PLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKT
                     AQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQ
                     MELTSHLLTAAAFGTMKNSEN"
BASE COUNT           76 a          127 c          102 g           65 t
ORIGIN      
        1 cctcttcacc agattgtgaa tcgcctggac atcatgctgc cgggcaatcg ccccttcgtt
       61 gcccccagtg ccgttattca ggcccgccag cgcctgggaa gtgaggctgt ccgccgcgtg
      121 ttcacgaaaa cagcgcagct ctggcataac gccacgccgc atccgcactg gtgcggcctg
      181 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
      241 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      301 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      361 agcgaaaatg
//
LOCUS       DAJTYG010000325          364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTYG010000325 DAJTYG010000000
VERSION     DAJTYG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.331"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
BASE COUNT           79 a          102 c          103 g           80 t
ORIGIN      
        1 cacctgtaga aattcgaatt agtgacacga caacacaggt gatatggcaa tgataacacg
       61 tcagatgagc catcgcccac aatcgggtgg tgcgcatgaa aaaccctaaa ctccagtacc
      121 tcactgatta ttccccctct gacgcgccct gggacgcaca cagaagcgtt tctgacgacg
      181 tgggagggat ttacctgctt gcggccgaat atgagcgcta cggggctcgt atggcctcct
      241 gtggcggttt actgcgtttc gggtggtcta ccctcaagga aaccggagaa acgcgtctga
      301 ggctgcgtga ggcgcatttc tgccgtgtcc ggcactgtcc tgtctgccag tggaggcgct
      361 ccct
//
LOCUS       DAJTYG010000326          360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTYG010000326 DAJTYG010000000
VERSION     DAJTYG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.332"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
BASE COUNT          134 a           65 c           79 g           82 t
ORIGIN      
        1 gatggggcgc ttacttcctg aacattccca gccatagaca aatccttcgc caaaattaaa
       61 ggggcatctg taaaacgagg gattatgcgc ggcaaataag aagtgcggaa atagcgaaat
      121 gcaaaattat aacctgctgc acattttatt tttaaacgaa taaaatttgg aaaaaagcaa
      181 gtcagataga agaaacaaaa aagcagagtg tgaaacagta agttcacact ttgcttgaat
      241 tcatttacac gtgagcaacg ggtttcggcg tatgaccggc gttgcgaagc agcatcagca
      301 aatagataaa cgatacgctg gcgatcatga taaacaataa attatcggag aaattctgca
//
LOCUS       DAJTYG010000327          358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTYG010000327 DAJTYG010000000
VERSION     DAJTYG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.333"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..>358)
                     /locus_tag="OO568_004230"
     CDS             complement(<1..>358)
                     /locus_tag="OO568_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6643283.1"
                     /translation="IAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYI
                     TTISMLVFAVGGAEKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDS
                     RNIPDDLMTNGQYYAFQ"
BASE COUNT          103 a           91 c           80 g           84 t
ORIGIN      
        1 ctgaaaggcg taatattgac cgttcgtcat taagtcatcc gggatattac gcgagtcaaa
       61 catcatcccc atcgccagtg agcccagaat ggcacaaacc gcaaccatca ccgccaggca
      121 tagcatccct tttggaaatt cttttcctgg gttgcgcgtt tgattaacgt aaggagaaat
      181 cttctctgct ccgccaaccg cgaaaaccag cattgaaata gtggtaatgt aggtaaagtc
      241 gatatgagga atgaacgttt cccaggtaat gtttgtggtc gcaatatgca cttcagtaat
      301 cgcaggcgcg gttaccgcca tcgccacata caggagcgac ataacaaaca tcgcaatc
//
LOCUS       DAJTYG010000328          353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTYG010000328 DAJTYG010000000
VERSION     DAJTYG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.335"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..208)
                     /locus_tag="OO568_004231"
     CDS             complement(<1..208)
                     /locus_tag="OO568_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR6643284.1"
                     /translation="MSKIDYQALRAKAEKATKGSYIVGHTSVNRHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVEVVNK"
     gene            complement(205..>353)
                     /locus_tag="OO568_004232"
     CDS             complement(205..>353)
                     /locus_tag="OO568_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HCR6643285.1"
                     /translation="PGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKYLNAMWRK
                     GGNQ"
BASE COUNT           81 a           94 c           64 g          114 t
ORIGIN      
        1 ttttgttgac cacttcaaca tctgtttcaa ccaggcagtt aacatgacat tccgcaatca
       61 caccaccggg ttctcctttc catttttgac aaacaaaaac tcctgttaaa ttgccgtgcc
      121 gattaacaga tgtatgccct acgatgtagc ttcctttagt tgctttttct gccttggcac
      181 gcagtgcctg atagtcaatc ttgctcactg gttgcctcct ttgcgccaca tcgcattcag
      241 atatttgttg tcattaacag aaccgaaact ctttctctta agcaattcct ctctcgatgg
      301 cattggcttt acgcgctggc gaataatcat ttctgccgga agaatgccgg gat
//
LOCUS       DAJTYG010000329          351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTYG010000329 DAJTYG010000000
VERSION     DAJTYG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.336"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..>351)
                     /locus_tag="OO568_004233"
     CDS             complement(<1..>351)
                     /locus_tag="OO568_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643286.1"
                     /translation="STAYVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNAL
                     AGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLG
                     LQETVNKADNAVQK"
BASE COUNT           73 a          102 c           95 g           81 t
ORIGIN      
        1 tcttttggac agcgttatca gccttgttta ccgtttcctg taaaccgagg tattcgataa
       61 cggcggcaac ggtcgatttc gcaagaatat cccgcccgac ttttgtcagg gttgccaggc
      121 tggcgacatc attccccgta aaatacggaa acctgtctgc cgcagtagca agccccgcca
      181 gcgccgtcag agtggcatct ttcggttgct tacccgcaag cgcgttagtc atggtggtcg
      241 caaaattcgg gtcgttgccc agcgccgccg ccagttcatt cagcgtgttc agtgcatcag
      301 gcgacgagtc cacgagggca gcaatcgcgg ccataacata agccgtactg g
//
LOCUS       DAJTYG010000330          349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTYG010000330 DAJTYG010000000
VERSION     DAJTYG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.337"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            <1..>349
                     /locus_tag="OO568_004234"
     CDS             <1..>349
                     /locus_tag="OO568_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6643287.1"
                     /translation="YREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
                     AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGR
                     AFPRVVKERPWKYP"
BASE COUNT          104 a           63 c          110 g           72 t
ORIGIN      
        1 gatacaggga gataaaacag acgatgcaac tgagcaggct gacgctgaga agtaaaaagc
       61 cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg gtgagatatc
      121 agatgattaa aatggcggaa catctgaaag gttactggcc gaatcaactg agtttctcag
      181 aatcatgcgg aatggtgatg agaatgctga tgacattgca gggcgcttca ccgggacgta
      241 taccggagct gatgcgcgat cttgcaagta tgggacaact tgtgaaatta ccgacgagaa
      301 ggggaagggc cttcccgaga gtggtaaagg agaggccctg gaaataccc
//
LOCUS       DAJTYG010000331          343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTYG010000331 DAJTYG010000000
VERSION     DAJTYG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.338"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            1..343
                     /locus_tag="OO568_004235"
                     /pseudo
     CDS             1..343
                     /locus_tag="OO568_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          102 a           78 c           85 g           78 t
ORIGIN      
        1 ccaacgttac tctacggagt ttaaagccga agccgtcaaa acggttcctg aaaatcagct
       61 ttcgatcagt gaaggcgctt cccgattatc tcttcctgaa ggcactttag gacaatgggt
      121 taccgccgca agaaaagggc tcaatacacc aggctcccgc acagtggctg agctggaatc
      181 tgaagttatg caactgcgta aggctttaaa tgaagcacga cttgagcgag atatattaaa
      241 aaaagcaaca gcgtattttg cacaggagtc gctgaaaaat acgcgttagt cgaacaatgg
      301 cgacaacaat tccctgttga agcgatgtgt cgggtacctg gtg
//
LOCUS       DAJTYG010000332          338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTYG010000332 DAJTYG010000000
VERSION     DAJTYG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.339"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(12..>338)
                     /gene="ospZ"
                     /locus_tag="OO568_004236"
     CDS             complement(12..>338)
                     /gene="ospZ"
                     /locus_tag="OO568_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCR6643288.1"
                     /translation="HALCELPEISCKIFSKENSYFLYIIVVFRNDSPQGELRANRFIE
                     LYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMNDRLSQIK
                     ARMPVT"
BASE COUNT          108 a           66 c           44 g          120 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aactctggcg actcatctct taacacttgc
      181 ataatctctc tttttatatc atatagttct ataaaccgat ttgcccgtag ttctccttgg
      241 ggagaatcat ttctgaatac aacaattata tacaaaaaat aactattttc tttagagaag
      301 atcttacaac taatctctgg gagctcacat aatgcatg
//
LOCUS       DAJTYG010000333          323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTYG010000333 DAJTYG010000000
VERSION     DAJTYG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.340"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(40..>323)
                     /locus_tag="OO568_004237"
     CDS             complement(40..>323)
                     /locus_tag="OO568_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643289.1"
                     /translation="FVLLNAQKLRDTQHMMQQILLSLVHALYEGNPQPVFANTEKLND
                     AVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT           87 a           76 c           74 g           86 t
ORIGIN      
        1 gaatcgaagc agcagcaaga tgattctgaa gttcaggaat tatttgcgca aggcccggca
       61 aatcagattc gatagcaact caagctgatg tgccgtttcc atgttcagcc acacataacc
      121 atagattggt gtttccacga ctttcaggtc atggtggtta tcgagcaact gacgcagctc
      181 ttccacagca tcgttcaatt tttccgtatt ggcaaaaacc ggctgcggat taccttcgta
      241 cagcgcatga acaaggctca gcagtatttg ctgcatcatg tgctgggtat cacgaagttt
      301 ttgcgcgttc aataacacga aat
//
LOCUS       DAJTYG010000334          318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTYG010000334 DAJTYG010000000
VERSION     DAJTYG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.denovo.341"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            complement(<1..>318)
                     /locus_tag="OO568_004238"
     CDS             complement(<1..>318)
                     /locus_tag="OO568_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR6643290.1"
                     /translation="RFMIITGVRPINVHNLRWDYVYEEAGEIVYPEGVIGMRGAMKTQ
                     KAFRLPITPEIRRIIDEQKAWRDSVPECNRDYVFLQPRDPMQPFSKRSLDKLVKTYSP
                     DGAV"
BASE COUNT           77 a           88 c           72 g           81 t
ORIGIN      
        1 tacagccccg tccgggctgt atgttttcac cagcttatcc agtgatcgtt ttgaaaatgg
       61 ctgcattgga tcacgtggct gcaaaaatac ataatccctg ttgcactcag gaactgaatc
      121 acgccaggct ttctgctcgt cgataatccg ccggatctca ggcgttatcg gcaggcggaa
      181 agccttttgt gttttcatag cccctcgcat gccgataacc ccttccggat aaacaatttc
      241 accagcctcc tcgtaaacgt agtcccagcg caggttatgg acattaatcg gacgaacacc
      301 agtgatgatc atgaagcg
//
LOCUS       DAJTYG010000335         6385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.guided.158, whole genome shotgun sequence.
ACCESSION   DAJTYG010000335 DAJTYG010000000
VERSION     DAJTYG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.guided.158"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            575..1390
                     /gene="sul2"
                     /locus_tag="OO568_004239"
     CDS             575..1390
                     /gene="sul2"
                     /locus_tag="OO568_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6643291.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1451..1978
                     /locus_tag="OO568_004240"
                     /pseudo
     CDS             1451..1978
                     /locus_tag="OO568_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            1999..2472
                     /locus_tag="OO568_004241"
     CDS             1999..2472
                     /locus_tag="OO568_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR6643292.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            2547..2822
                     /locus_tag="OO568_004242"
                     /pseudo
     CDS             2547..2822
                     /locus_tag="OO568_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            2822..3658
                     /locus_tag="OO568_004243"
     CDS             2822..3658
                     /locus_tag="OO568_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6643293.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3765..4169)
                     /locus_tag="OO568_004244"
                     /pseudo
     CDS             complement(3765..4169)
                     /locus_tag="OO568_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4213..4602)
                     /locus_tag="OO568_004245"
     CDS             complement(4213..4602)
                     /locus_tag="OO568_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643294.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4820..5260
                     /locus_tag="OO568_004246"
     CDS             4820..5260
                     /locus_tag="OO568_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6643295.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(6005..6154)
                     /locus_tag="OO568_004247"
     CDS             complement(6005..6154)
                     /locus_tag="OO568_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6643296.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1588 a         1650 c         1652 g         1495 t
ORIGIN      
        1 taactatcaa cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat
       61 tagtaacagg agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg
      121 gctacactga aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag
      181 cagttcccca actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta
      241 cagagcaaga gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta
      301 tcaattaatc ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa
      361 caggtttccc tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct
      421 acttattgag agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg
      481 atcaccgcaa acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga
      541 ctcatgctcg attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg
      601 catcgtcaac ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc
      661 agccattgcg caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc
      721 ggcatccagc aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc
      781 gccggtgctg gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc
      841 cgcgacgcaa gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt
      901 tccagacgct gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca
      961 ttcggtgcaa gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca
     1021 cattgcggcg ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa
     1081 ccgccttgtc cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct
     1141 ctcggtgctg gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc
     1201 tgtttcgcgc aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc
     1261 cgcgacactc gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca
     1321 cgagccgcgc cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag
     1381 aattcgttaa ctgcacattc gggatatttc tctatattcg cgcttcatca gaaaactgaa
     1441 ggaacctcca ttgaatcgaa ctaatatttt ttttggtgaa tcgcattctg actggttgcc
     1501 tgtcagaggc ggagaatctg gtgattttgt ttttcgacgt ggtgacgggc atgccttcgc
     1561 gaaaatcgca cctgcttccc gccgcggtga gctcgctgga gagcgtgacc gcctcatttg
     1621 gctcaaaggt cgaggtgtgg cttgccccga ggtcatcaac tggcaggagg aacaggaggg
     1681 tgcatgcttg gtgataacgg caattccggg agtaccggcg gctgatctgt ctggagcgga
     1741 tttgctcaaa gcgtggccgt caatggggca gcaacttggc gctgttcaca gcctatcggt
     1801 tgatcaatgt ccgtttgagc gcaggctgtc gcgaatgttc ggacgcgccg ttgatgtggt
     1861 gtcccgcaat gccgtcaatc ccgacttctt accggacgag gacaagagta cgccgcagct
     1921 cgatcttttg gctcgtgtcg aacgagagct accggtgcgg ctcgaccaag agcgcaccgt
     1981 taacccagga tgagaacctt gaaagtatca ttgatagctg cgaaagcgaa aaacggcgtg
     2041 attggttgcg gtccagacat accctggtcc gcgaaagggg agcagctact ttttaaagca
     2101 ttgacctaca atcagtggct tctggtgggt cgcaagacgt ttgaatctat gggcgcactc
     2161 cccaatagga aatacgcggt cgttacccgc tcaggttgga catcaaatga tgacaatgta
     2221 gttgtatttc agtcaatcga agaggccatg gacaggctag ctgaattcac cggtcacgtt
     2281 atagtgtctg gtggcggaga aatttaccga gaaacattac ccatggcctc tacgctccac
     2341 ttatcgacga tcgacatcga gccagagggg gatgttttct tcccgagtat tccaaatacc
     2401 ttcgaagttg tttttgagca acactttact tcaaacatta actattgcta tcaaatttgg
     2461 aaaaagggtt aacaaagcta tgcaatcgac ggcaaaaagc ttcgttcgct tcgcgcacta
     2521 cgcctttttc cgcgattgat agcgacgata tggttgtttg ccatggtgat ccctgcatgc
     2581 cgaacttcat ggtggaccct aaaactcttc aatgcacggg tctgatcgac cttgggcggc
     2641 tcggaacagc agatcgctat gccgatttgg cactcatgat tgctaacgcc gaagagaact
     2701 gggcagcgcc agatgaagca gagcgcgcct tcgctgtcct attcaatgta ttggggatcg
     2761 aagcccccga ccgcgaacgc cttgccttct atctgcgatt ggaccctctg acttggggtt
     2821 gatgttcatg ccgcctgttt ttcctgctca ttggcacgtt tcgcaacctg ttctcattgc
     2881 ggacaccttt tccagcctcg tttggaaagt ttcattgcca gacgggactc ctgcaatcgt
     2941 caagggattg aaacctatag aagacattgc tgatgaactg cgcggggccg actatctggt
     3001 atggcgcaat gggaggggag cagtccggtt gctcggtcgt gagaacaatc tgatgttgct
     3061 cgaatatgcc ggggagcgaa tgctctctca catcgttgcc gagcacggcg actaccaggc
     3121 gaccgaaatt gcagcggaac taatggcgaa gctgtatgcc gcatctgagg aacccctgcc
     3181 ttctgccctt ctcccgatcc gggatcgctt tgcagctttg tttcagcggg cgcgcgatga
     3241 tcaaaacgca ggttgtcaaa ctgactacgt ccacgcggcg attatagccg atcaaatgat
     3301 gagcaatgcc tcggaactgc gtgggctaca tggcgatctg catcatgaaa acatcatgtt
     3361 ctccagtcgc ggctggctgg tgatagatcc cgtcggtctg gtcggtgaag tgggctttgg
     3421 cgccgccaat atgttctacg atccggctga cagagacgac ctttgtctcg atcctagacg
     3481 cattgcacag atggcggacg cattctctcg tgcgctggac gtcgatccgc gtcgcctgct
     3541 cgaccaggcg tacgcttatg ggtgcctttc cgcagcttgg aacgcggatg gagaagagga
     3601 gcaacgcgat ctagctatcg cggccgcgat caagcaggtg cgacagacgt catactagat
     3661 atcaagcgac ttctcctatc ccctgggaac acatcaatct caccggagaa tatcgctggc
     3721 caaagcctta gcgtaggatt ccgccccttc ccgcaaacga ccccaaacag gaaacgcagc
     3781 tgaaacggga agctcaacac ccactgacgc atgggttgtt caggcagtac ttcatcaacc
     3841 agcaaggcgg cactttcggc catccgccgc gccccacagc tcgggcagaa accgcgacgc
     3901 ttacagctga aagcgaccag gtgctcggcg tggcaagact cgcagcgaac ccgtagaaag
     3961 ccatgctcca gccgcccgca ttggagaaat tcttcaaatt cccgttgcac atagcccggc
     4021 aattcctttc cctgctctgc cataagcgca gcgaatgccg ggtaatactc gtcaacgatc
     4081 tgatagagaa gggtttgctc gggtcggtgg ctctggtaac gaccagtatc ccgatcccgg
     4141 ctggccgtcc tggccgccac atgaggcatg ttccgcgtcc ttgcaatact gtgtttacat
     4201 acagtctatc gcttagcgga aagttctttt accctcagcc gaaatgcctg ccgttgctag
     4261 acattgccag ccagtgcccg tcactcccac tcttacattg ttcgcagtat ctaggtacat
     4321 gttattgcgt gcaccttcat gatgtataat gttattaatg acagcagaaa gatcgacaga
     4381 attaatattc ttcgtacctg ggacagttat cgggtgtcgg ccagtgatga gaccaaagga
     4441 tgcagtataa gatgaaccac tactattatc atggccacca ttaccaccac cgttaaaact
     4501 acctcccgaa cttccagagc cgctatgacc atcattgtag gggtcatttg gtcccatgtg
     4561 accaaaacca ccgacagtac cgctatttaa ctctgtactc ataaaattat cctctttgac
     4621 ttttaaaaac agcaaaaact gtatttataa caatgtgttt atatacagta ttcgaaatac
     4681 tacgcccgtc aaaaccacac cgtcaacagg ttttttgtgt atttttcacg cctcacgggc
     4741 agcctcagac cttttgtgtt aaaacagtaa tatgttctgc tgttacaata cggtacacac
     4801 aggagacact gtcactttta tggcatggca cacagttcaa gagacactga aattaacagg
     4861 aaagtcgcgc tcgcaattat atcgtgacat ggctaaaggc cttgtcagct atcgcacagg
     4921 aaaaaatgat cgccgggagt tcgagaccag tgaactgatt cgggcctatg gcgagctgcg
     4981 cacttctgag acaccaaaaa ggcacaagga gggacactcc aaatctgtcg ttagccaggc
     5041 ggaaaatgct cagctagacg tcattcagca gaaactggac gctctgcagc agactgttac
     5101 cctaatgctt gaagacaaga tggcgcggga agcagaaaac cgcctgcgaa atgaggagag
     5161 ggaacaacta caggccgaaa ttagccgtct ggctgacgcg ctggcacagg aaaaaaagcg
     5221 gggattctgg tcgcggctgt ttcgctccgg ttcagagtaa cagtgcaggt gactctgatt
     5281 taccataaca tctgttatca gcaccgcctg agaggccctc agggcgtttc tgacgggaaa
     5341 ccggtaaaac aggcaacaaa ccacccgaaa acccgccaga aacgcgcagg ctctgttttt
     5401 acggcatgca tgagtatgcg cacaagtgca tagcgcggaa attcacacca ttcagccggg
     5461 gtaagtcctg tttccggcgt tcttcccatc cgaaaacggt gaacggggac gcaatttatt
     5521 gcgtggccgt gaagcgttgc cggcgcagcc ggaaaacccg ctgtcagcac gttcaggaag
     5581 ggggtgatgt acaaatggca cagaggatga aagtgacgta tgagctgaag gaatgatgta
     5641 aaagtgatgt ataagtgatg taaaagtgat gtatgaactg aaggagcgat gtaattctgt
     5701 agtcaggaag aataacccgt tcatgcaggc atgaacgata cagtgaggaa cgcttttttt
     5761 gtcacgatct ccccttaacc tgcgtaaatc acccgccatg gttgattatt tttcaacctc
     5821 aaaatggtca tacacctaac gcagccgcga ttttcatcgc aactgcatga attaaaagaa
     5881 aaaaaaggaa tatttcgttt tcacgagtaa tacccgggta ttaaaaatcc actgtaatta
     5941 catgaaaata aagaaataaa aggtaatacc ggagtattac cacgtacgcg ggcaagcgaa
     6001 aaagctataa cgcttcccgg ccgacaggcg acccaaaatc cacaggggca tgcaggttct
     6061 cttcagtaac ctgcgccatt aaatcaaaaa ggtcatattc gccgggaagc tgcgtatcag
     6121 tcgaatccag ttgttcatca tattcagatg gcatggggtg atatccagta gctgagaggt
     6181 tgttagcggg tttcggggcg cagccctgaa ccagtcatgt agcgctagcg gagtgtatac
     6241 tggcttacta tgttgccacg attgcgaaaa tcagtgaagt gcttcatgtg gcaggagaaa
     6301 aaaggcagca ccggcgcgtc agcagaatat gcgatacagg agatattccg cttcctcgct
     6361 cactgactcg ctacgctcgg tcgtt
//
LOCUS       DAJTYG010000336         5106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.guided.182, whole genome shotgun sequence.
ACCESSION   DAJTYG010000336 DAJTYG010000000
VERSION     DAJTYG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.guided.182"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            68..802
                     /gene="frdB"
                     /locus_tag="OO568_004248"
     CDS             68..802
                     /gene="frdB"
                     /locus_tag="OO568_004248"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6643297.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            813..1208
                     /gene="frdC"
                     /locus_tag="OO568_004249"
     CDS             813..1208
                     /gene="frdC"
                     /locus_tag="OO568_004249"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6643298.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1219..1578
                     /gene="frdD"
                     /locus_tag="OO568_004250"
     CDS             1219..1578
                     /gene="frdD"
                     /locus_tag="OO568_004250"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6643299.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            1641..2774
                     /locus_tag="OO568_004251"
     CDS             1641..2774
                     /locus_tag="OO568_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6643300.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWIHVPPPEKKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            2863..3396
                     /gene="blc"
                     /locus_tag="OO568_004252"
     CDS             2863..3396
                     /gene="blc"
                     /locus_tag="OO568_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6643301.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3393..3622)
                     /locus_tag="OO568_004253"
                     /pseudo
     CDS             complement(3393..3622)
                     /locus_tag="OO568_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(3798..3944)
                     /gene="ecnB"
                     /locus_tag="OO568_004254"
     CDS             complement(3798..3944)
                     /gene="ecnB"
                     /locus_tag="OO568_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6643302.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(4055..4180)
                     /gene="ecnA"
                     /locus_tag="OO568_004255"
     CDS             complement(4055..4180)
                     /gene="ecnA"
                     /locus_tag="OO568_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6643303.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(4232..4798)
                     /gene="efp"
                     /locus_tag="OO568_004256"
     CDS             complement(4232..4798)
                     /gene="efp"
                     /locus_tag="OO568_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6643304.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            4840..>5106
                     /locus_tag="OO568_004257"
     CDS             4840..>5106
                     /locus_tag="OO568_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6643305.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGF"
BASE COUNT         1279 a         1300 c         1307 g         1220 t
ORIGIN      
        1 cgcgtttacg gtggcgaagc ggatgcagcc gataaggcgg aagcagccaa taataaggag
       61 aaggcgaatg gctgagatga aaaacctgaa aattgaggtg gtgcgctata acccggaagt
      121 cgataccgca ccgcatagcg cattctatga agtgccttat gacgcaacta cctcattact
      181 ggatgcgctg ggctacatca aagacaacct ggcaccggac ctgagttacc gctggtcctg
      241 ccgtatggcg atttgtggtt cctgcggcat gatggttaac aacgtgccaa aactggcatg
      301 taaaaccttc ctgcgtgatt acaccgacgg tatgaaggtt gaagcgttag ctaacttccc
      361 gattgaacgc gatctggtgg tcgatatgac ccacttcatc gaaagtctgg aagcgatcaa
      421 accgtacatc atcggcaact cccgcaccgc ggatcagggt actaacatcc agaccccggc
      481 gcagatggca aagtatcacc agttctccgg ttgcatcaac tgtggtctgt gctacgccgc
      541 gtgcccgcag tttggcctga acccagagtt catcggtccg gctgccatta cgctggcgca
      601 tcgttataac gaagatagcc gcgaccacgg taagaaggag cgtatggcgc agttgaacag
      661 ccagaacggt gtatggagct gtactttcgt gggctattgc tccgaagtct gcccgaaaca
      721 cgtcgatccg gctgcggcca ttcagcaggg caaagtagaa agttcgaaag actttcttat
      781 cgcgaccctg aaaccacgct aaggagtgca acatgacgac taaacgtaaa ccgtatgtac
      841 ggccaatgac gtccacctgg tggaaaaaat tgccgtttta tcgcttttac atgctgcgcg
      901 aaggcacggc ggttccggct gtgtggttca gcattgaact gattttcggg ctgtttgccc
      961 tgaaaaatgg cccggaagcc tgggcgggat tcgtcgactt tttacaaaac ccggttatcg
     1021 tgatcattaa cctgatcact ctgacggcag ctctgctgca caccaaaacc tggtttgaac
     1081 tggcaccgaa agcggccaat atcattgtaa aagacgaaaa aatgggacca gagccaatta
     1141 tcaaaagtct ctgggcggta actgtggttg ccaccatcgt aatcctgttt gttgccctgt
     1201 actggtaagg agcctgagat gattaatcca aatccaaagc gttctgacga accggtattc
     1261 tggggcctct tcggggccgg tggtatgtgg agcgcaatta ttgcgccggt gatgatcctg
     1321 ctggtgggta ttctgctgcc actggggctg tttccgggtg atgcgctgag ctacgagcgc
     1381 gttctggcgt tcgcgcagag cttcattggt cgcgtattcc tgttcctgat gatcgttctg
     1441 ccgctgtggt gtggtttaca ccgtatgcac cacgcgatgc acgatctgaa aatccacgtg
     1501 cctgcgggca aatgggtttt ctacggtctg gctgctatcc tgacagttgt cacgctgatt
     1561 ggtgtcgtta caatctaacg catcgccaat gtaaatccgg cccgcctatg gcgggccgtt
     1621 ttgtatggaa accagacctt atgttcaaaa cgacgctctg cgccttatta attaccgcct
     1681 cttgctccac atttgctgcc cctcaacaaa tcaacgatat tgtgcatcgc acaattaccc
     1741 cgcttataga gcaacaaaag atccccggta tggcggtggc ggtaatttat cagggtaaac
     1801 cttattactt tacctggggc tatgcggaca tcgccaaaaa gcagcccgtc acacagcaaa
     1861 cgttgtttga gttaggttcg gtcagcaaaa catttacggg cgtgcttggt ggcgacgcta
     1921 ttgctcgagg ggaaatcaag ttaagcgatc ccgcaacaaa atactggcct gaacttaccg
     1981 ctaaacagtg gaatgggatc acactattac atcttgcgac ctacaccgct ggcggcctgc
     2041 cattgcaggt gccggatgaa gtgaaatcct caagcgactt gctgcgcttc tatcaaaact
     2101 ggcagcctgc atgggctcca ggaacacaac gtctgtatgc caactccagt atcggtttgt
     2161 tcggcgcact ggctgtgaag ccgtctggtt tgagttttga gcaggcgatg caaactcgtg
     2221 tcttccagcc actcaaactc aaccatacgt ggattcatgt acctccccca gaaaaaaaga
     2281 attacgcctg gggatatcgc gaaggtaagg cagtgcatgt ttcgccaggg gcgttagatg
     2341 ctgaagctta tggtgtgaag tcgaccattg aagatatggc ccgctgggtg caaagcaatt
     2401 taccccccct tgatatcaat gagaaaacgc ttcaacaagg gatacaactg gcacaatctc
     2461 gctactggca aaccggcgat atgtatcagg gcctgggctg ggaaatgctg gactggccgg
     2521 tcaatcctga cagcatcatt aacggcagtg acaataaaat tgcactggca gcacgcccgg
     2581 taaaagcgat taagccccca actcctgcag tacgcgcatc atgggtacat aaaacggggg
     2641 cgaccggcgg atttggtagc tatgtcgcgt ttattccaga aaaagagctg ggtatcgtga
     2701 tgctggctaa caaaaactat cccaatccag agagagtcgc cgccgcctgt cagattctca
     2761 acgctctaca gtaaaatttc atcgggtccg aattttcgga ccttttctgc gcttttcctt
     2821 gctgtcatct acacttagaa aaaaaccagt aaggaaacat ttatgcgcct gctccctctc
     2881 gttgccgcag cgacagctgc atttctggtc gttgcctgta gttctcctac gccgccgcgt
     2941 ggcgtgaccg tagtgaataa tttcgacgcc aaacgttatc tcggtacctg gtatgagatt
     3001 gcccgttttg atcaccgttt tgaacgtgga ctggaaaaag tcaccgcaac atacagcctg
     3061 cgtgatgacg gcggcctgaa tgtcattaat aaaggctata accctgacag aggaatgtgg
     3121 cagcagagtg aagggaaagc gtactttacc ggcgcaccaa ctcgcgctgc gctgaaagtg
     3181 tcattctttg gtcctttcta tggcggttat aacgttattg cactcgatcg ggaataccgc
     3241 catgcgctgg tttgcggccc ggaccgcgac tacctgtgga tactctcccg cacgccaacc
     3301 atttctgacg aagtgaaaca ggagatgctg gcagtcgcga cccgggaagg gtttgatgtc
     3361 agtaagttta tttgggtaca gcagcccggt agttagtgag tgctgagttt cagaccaata
     3421 atccccaata cgattaacgc cagactcgcc agacgcatcg ggttagcgga ctcaccgagc
     3481 agcacaatgc cggtgatggc cgcgccgact gcgccaatac ccgtccacac ggcatcaaac
     3541 ggctaaagcc gtgggtatat ttcaggccaa cggcccatac cacttccagc agaccagcaa
     3601 taactaagat aatccaggac atatcaggct ccggaaattt ggggccgtcc ccggtgaaaa
     3661 gaggcgtttg caggtcgtcc tgcaaagctg atggttgaac ggatattttg ccaggtggca
     3721 ggggaatttc aactattacc tttatgacga tcgcagaaaa aacaaacggc acgacactgc
     3781 tgtcgtaccg ttattgctta ttgctgcgct ttcgttgctg cgccagaaat cgcgttaccg
     3841 ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa tactgttgaa
     3901 agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc ttccttttag
     3961 gtaatgttat ttatgtttgc ctatagcaaa ttaataatag acaacatcgc caaaaatgat
     4021 ggaaatctga tgatttttcg gaagagaaga aaaattagct ggcagcgcga gagatggagt
     4081 tgccgagatg tttgatgtct tcgccgaaac cgcaagcggt gttacagccc gtgagcagcg
     4141 tgctggcaag caaaacaaga acgataaggc gtttcatcat ctctgacctg aataagtgat
     4201 ggtgcagcct gcaggccgca ccacaaccgc attacttcac gcgagagacg tattcaccag
     4261 agcgggtatc cactttgatg acttcgccga tttgtacgaa cagcggaact ttaaccacag
     4321 cgccagtaga cagggtagcc ggtttgccgc cagtacctgc ggtatcacct ttcaggcccg
     4381 gatcggtatc aacgatttcc agttcaacga agttcggcgg agtaacggag atcggctgac
     4441 cattccacag agttacgata cactctgcct gatccagcag ccatttagcg ttgtcgccaa
     4501 ttgcttttgc atcagcagac agctgctcga aagtttcgtt gttcatgaag tgccagaact
     4561 caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac atcagcgcct tcagcggaat
     4621 cagtagattt gaaggttttt tctacgcgag taccggtcag cagacgacgc agtttaacgc
     4681 gagcaaatgc ctggccttta cccggtttta cgaattcact cgcttcaacc gcgtaaggtt
     4741 cgccgtctaa catgatttta agaccagcac gaaaatcgtt gctatagtac gttgccataa
     4801 ggccctctga aatttgttaa ttggtagcta agccacaaaa tggcgcatat tgtaacccta
     4861 aataccccat ccagagaaga ttggttaacg caacttgccg atgttgtgac cgatcctgat
     4921 gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac tgttggccgg acgcagcgcc
     4981 aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg atcgcatgga gaaaggcaat
     5041 ccggacgatc ctcttttgcg tcaggtactt acctcgcaag atgagtttgt cgtcgcgtcc
     5101 ggattc
//
LOCUS       DAJTYG010000337         1803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 300209
            SAMEA8899960-rid15816193.guided.244, whole genome shotgun sequence.
ACCESSION   DAJTYG010000337 DAJTYG010000000
VERSION     DAJTYG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899960
            Sequence Read Archive: ERR6004933
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477847.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,174
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 85
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 188 of 390
            Pseudo Genes (incomplete)         :: 99 of 390
            Pseudo Genes (internal stop)      :: 141 of 390
            Pseudo Genes (multiple problems)  :: 36 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899960-rid15816193.guided.244"
                     /serovar="S. flexneri 3a"
                     /isolate="300209"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mozambique"
                     /collection_date="2008-01-25"
     gene            122..580
                     /gene="gpt"
                     /locus_tag="OO568_004258"
     CDS             122..580
                     /gene="gpt"
                     /locus_tag="OO568_004258"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6643306.1"
                     /translation="MSEKYIVTWDRLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            672..>1803
                     /gene="frsA"
                     /locus_tag="OO568_004259"
     CDS             672..>1803
                     /gene="frsA"
                     /locus_tag="OO568_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6643307.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLI"
BASE COUNT          407 a          461 c          487 g          448 t
ORIGIN      
        1 aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatatct ataatctcgc
       61 gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca
      121 catgagcgaa aaatacatcg tcacctggga caggttgcag atccatgcac gtaaactcgc
      181 aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct
      241 ggtaccgggg gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
      301 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
      361 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg ctgttgcgat
      421 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc
      481 gctggttgat gactatgttg ttgatatccc gcaagatacc tggatcgaac agccgtggga
      541 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcatt
      601 gccgggcgtt gttcttttta acttcaggca ggttacaata gtttccagta agtattctgg
      661 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
      721 atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg tgcacaaccg cctgtgcagt
      781 cggctcttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
      841 tctggcgcgg tattgaccca cgtgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg
      901 atgctgaacg taccgacgat gatttatacg ataccgtgat tggctaccgt ggtggcaact
      961 ggatttatga gtgggccacc caggcgatgg tgtggcaaca aaaagcctgt acggaagaag
     1021 atccgcaact cagtggtcga cactggctgc atgcggctac gttgtacaac attgccgcct
     1081 atcctcatct gaaaggagat gacctggccg agcaagcgca ggctttatca aaccgcgcct
     1141 atgaagaggc cgcccagcgt ctgccgggca cgatgcggca gatggagttt accgtacccg
     1201 gcggtgcgcc catcaccggc tttttgcata tgccgaaagg cgatggcccg ttcccgacag
     1261 tattaatgtg tggtggtctg gatgcgatgc agacggacta ttacagcctg tatgaacgtt
     1321 attttgcgcc gcgcggcatt gcgatgctga ctattgatat gccgtcggtg ggcttttctt
     1381 caaaatggaa gctcacccag gactccagcc tgttgcatca gcacgtctta aaggcgctgc
     1441 ctaacgtacc gtgggtggat cacactcgcg tcgcggcctt tggtttccgt ttcggcgcta
     1501 acgttgccgt gcgtttggca taccttgaat cgccgcgtct gaaagcggtt gcctgtcttg
     1561 gtccggtagt tcataccctg ttgagtgatt ttaagtgcca gcaacaggtg ccggaaatgt
     1621 atcttgacgt tctggcgagt cgtttgggga tgcatgatgc ttccgacgaa gcgttacgcg
     1681 tggagctgaa tcgctattca ttaaaagtgc aaggattgct ggggcgtcgc tgcccaacgc
     1741 caatgttatc aggctactgg aagaacgatc cgttcagccc ggaagaggac tcacgcttaa
     1801 tca
//
